LOCUS       DACGLE010000001       122330 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0534
            SAMEA3304449-rid9560453.denovo.001, whole genome shotgun sequence.
ACCESSION   DACGLE010000001 DACGLE010000000
VERSION     DACGLE010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304449
            Sequence Read Archive: ERR985064
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 122330)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 122330)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702225.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702225.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 20:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,045
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 27 of 67
            Pseudo Genes (incomplete)         :: 25 of 67
            Pseudo Genes (internal stop)      :: 19 of 67
            Pseudo Genes (multiple problems)  :: 4 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..122330
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304449-rid9560453.denovo.001"
                     /strain="VREN0534"
                     /db_xref="taxon:1352"
     gene            complement(<1..561)
                     /locus_tag="IXT20_000001"
     CDS             complement(<1..561)
                     /locus_tag="IXT20_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF443 family protein"
                     /protein_id="HAQ4858484.1"
                     /translation="METVRLWIFMKNFRSALRYVILESKSGYYLIDTDHYTFWGGYLF
                     PIFNWIIPHKVMKINLPAEEINRLILNKDLQRKREKEHSNVAWIGPAFAVVTSSIVGP
                     MLESIYSDFSIGIRYLFVTLSIAVLLCIKGYVSKKNKDILTLIGVNNLSSTKIILFPE
                     SIKEGSRLLIIFIMLILFSVLFGVMFI"
     gene            complement(748..1398)
                     /locus_tag="IXT20_000002"
     CDS             complement(748..1398)
                     /locus_tag="IXT20_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4858485.1"
                     /translation="MFIKGGQKMCFFTTFTIIMIAVLFIFLDIAKRNTAFLLYRVLLR
                     AGLITFISIVGFFLFTVIVFIWRTTAPPLPEITYGEFPFSLEYEIDEERILIEDTVIA
                     EFIGSFRGNVTTQPRRSWSIKLLNNSGELKAFHYEFLIIESEDVTITLSSGSAGYFMN
                     DGGSKPQLTVRDRIAGFMHFSHYDLEEARKILAEYGITLINWEYAPPIRNFFNDYY"
     gene            complement(1941..2876)
                     /gene="whiA"
                     /locus_tag="IXT20_000003"
     CDS             complement(1941..2876)
                     /gene="whiA"
                     /locus_tag="IXT20_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HAQ4858486.1"
                     /translation="MSFAADVKKELTGLEVHREHAKAELAALLRMNGSLGIVNQQFIL
                     NVQTENAAIARRIYSLLKDHYQVRSELLVRKKMKLKKNNVYIVRLKQGTKDVLMDLDI
                     MDGMMFNGHVSDEIMGNSQKMRSYLRGAFMASGSVNNPETSRYHLEIFSIYEQHNQDI
                     CDMLNYYGLHARTLERRNGYISYLKGAEKIADFLTLIGATNSMLRFEDVRIVRDMRNS
                     VNRLVNCETANLNKTIDAASKQIENIELIEAKVGLHALPEKLQEIAELRLQHPEVSLK
                     ELGEMIPSGAISKSGINHRIRKINEFAEKLREEAV"
     gene            complement(2910..3908)
                     /locus_tag="IXT20_000004"
     CDS             complement(2910..3908)
                     /locus_tag="IXT20_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HAQ4858487.1"
                     /translation="MKMKTYRIRKPKIVVVGGGTGLPVILKSLRNQGADITAVVTVAD
                     DGGSSGAIRESIAMAPPGDLRNVLVALSDMPQFYEDIFQYRFKKEDQFLANHTIGNLI
                     IAAVSEMRGSTYEAIQLLAKMMHVDGHVYPSSETPLTLHAVFKDGTTAVGESKIAINR
                     KTIDRVYVCNTNDKSEAKAARKVVSAIMDADMVVLGPGSLFTSILPNLVIPEIGEAML
                     KTTAETVYICNIMTQKGETEHFTDADHIRVLHKHLNQPFIDTVLVNTEKVPEDYMDPE
                     IYDEYLVQVAHDFNGLRNEGCRVISTDFLELKDGGVFHDGDKVVEELFRLVFGSKY"
     gene            complement(3905..4789)
                     /gene="rapZ"
                     /locus_tag="IXT20_000005"
     CDS             complement(3905..4789)
                     /gene="rapZ"
                     /locus_tag="IXT20_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HAQ4858488.1"
                     /translation="MTDNLQLVIITGMSGAGKTVAIQSFEDMGYFCIDNMPPRLIPKF
                     WELIKESGKVTKMALGVDLRSRSFFEEIQNMLIEIENTNFIDTSILFLDASDEELVAR
                     YKETRRAHPMAMDGLITEGIRKERAILDDLKAQASVIIDTTTLTPRQLREKINESFKS
                     KGDQGFRIEFVSFGFKYGLPIDSDIVMDVRFLPNPHYIPELRPLTGKDQPVYDYVMSF
                     DETQSFYHKFLDLLETVLPGYIKEGKSSLTVAIGCTGGQHRSVALTERLANALGKNYK
                     VNVTHRDKDKRKETVNRS"
     gene            complement(4924..5655)
                     /locus_tag="IXT20_000006"
     CDS             complement(4924..5655)
                     /locus_tag="IXT20_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate racemase"
                     /protein_id="HAQ4858489.1"
                     /translation="MENFFSILGGMGTMATESFVRLINHRTKATKDQEYLNYVLFNHA
                     TVPDRTAYILDRSEENPMPFLLDDIEKQNLLRPNFIVLTCNTAHYFFEELQAATDIPI
                     LHMPREAANELVRQHTTGRVAILGTEGSMKAGIYEREVKNLGFETVIPDTALQEKINY
                     LIYHEIKESDYLNQELYYEILEEAVERLNCEKVILGCTELSLMHEFAEDNHYPVIDAQ
                     SILADRTIERALAERSEALDTASEK"
     gene            complement(5659..6924)
                     /locus_tag="IXT20_000007"
     CDS             complement(5659..6924)
                     /locus_tag="IXT20_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aspartate ligase"
                     /protein_id="HAQ4858490.1"
                     /translation="MMNSIENEEFIPILLGSDMNVYGMARSFNEAYGKICQAYASDQL
                     APTRYSKIVNVEVIPGFDKDPVFIETMLRLAKERYSDKSKKYLLIACGDGYAELISQH
                     KQELSEYFICPYIDYSLFERLINKVSFYEVCEEYDLPYPKTLIVREEMLVNGHLEQEL
                     PFEFPVALKPANSVEYLSVQFEGRKKAFILETREEFDLILGRIYEAGYKSEMIVQDFI
                     PGDDSNMRVLNAYVDEDHQVRMMCLGHPLLEDPTPASIGNYVVIMPDYNEKIYQTIKA
                     FLEKIEYTGFANFDMKYDPRDGEYKLFEINLRQGRSSFFVTLNGLNLARFVTEDRVFN
                     KPFVETTYGTNQSDKARLWMGVPKKIFLEYARENEDKKLAEQMIKENRYGTTVFYEKD
                     RSIKRWLLMKYMFHNYIPRFKKYFHVKEG"
     gene            complement(7187..10003)
                     /gene="uvrA"
                     /locus_tag="IXT20_000008"
     CDS             complement(7187..10003)
                     /gene="uvrA"
                     /locus_tag="IXT20_000008"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HAQ4858491.1"
                     /translation="MANDKITIHGARAHNLKNIDVTIPRDKFVVVTGLSGSGKSSLAF
                     DTLYAEGQRRYVESLSAYARQFLGQMDKPDVDSIDGLSPAISIDQKTTSKNPRSTVGT
                     VTEINDYLRLLFARVGHPICPNDHIEITSQSVDQMADQVMALPERTKIQVLAPIVVKK
                     KGQHKKIFERIQKEGYVRVRVDGEIYDLSEVPELEKNKKHDIAIVIDRIIVKEGIRSR
                     LFDSLEAALRLADGYALVDVIGEEEMLFSEHYACPYCGFTVGELEPRLFSFNAPFGAC
                     PDCDGLGVKLEVDQDLVIPDRTKTLSQGAIVPWNPISSQYYPQMLAQACESFGIDLDT
                     PFEELPEEHQEIILNGTDDLFHFHYENDFGGVRDVETTFEGVLKNIERRYHDTNSDFT
                     REQMRLYMTELTCQTCKGYRLNPQALSVQINGTNIGQTNELSIQKAVDFFSNLTLSEQ
                     EKVIAKPILKEVNDRLSFLENVGLDYLTLSRASGTLSGGEAQRIRLATQIGSNLSGVL
                     YILDEPSIGLHQRDNDRLIGSLKKMRDLGNTLVVVEHDEDTMWAADYLIDVGPGAGEQ
                     GGQIVAAGTPEEVANDENSLTGKYLSGKKSIPVPKERRKGNGKAIKITGASENNLKNI
                     SVEFPLGEFVAVTGVSGSGKSTLVNSILKKSLAQKLNKNSAKPGKFKTISGYESIEKI
                     IDIDQSPIGRTPRSNPATYTSVFDDIRGLFAQTNEAKMRGYKKGRFSFNVKGGRCEAC
                     RGDGIIKIEMHFLPDVYVPCEVCHGKRYNSETLEVHYKGKSIADILEMTVEDAVEFFK
                     HIPKIHRKLQTIVDVGLGYVTMGQPATTLSGGEAQRMKLASELHKISNGKNFYILDEP
                     TTGLHSDDIARLLHVLQRLVDAGNTVLVIEHNLDVIKTADYIIDLGPEGGEGGGTILA
                     TGTPEEIINVKESYTGHYLKKIMV"
     gene            complement(10013..12007)
                     /gene="uvrB"
                     /locus_tag="IXT20_000009"
     CDS             complement(10013..12007)
                     /gene="uvrB"
                     /locus_tag="IXT20_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HAQ4858492.1"
                     /translation="MIERDTSRHFELVSKYQPAGDQPEAINQLVDGVVGGKKAQILLG
                     ATGTGKTYTISNLIEKVNKPTLIIAHNKTLAGQLYGEFKEFFPNNAVEYFVSYYDYYQ
                     PEAYVPSSDTYIEKDSSVNDEIDKLRHSATSSLLERNDVIVIASVSCIFGLGSPFEYQ
                     KQVVSIRQGAELDRNQLIRDLVSIQFERNDIDFQRGRFRVRGDVVEIFPASRDERALR
                     VEFFGDEVERIREVNALTGEVLGETEHVAIFPATHFVTNDEHMEHAVANIKEELEQRL
                     TVLRNENKLLEAQRLEQRTNYDIEMMLEMGYTSGIENYSRHMDGRKEGEPPYTLLDFF
                     PEDFLIVADESHVTMPQIRGMYNGDRARKQMLVDYGFRLPSALDNRPLRLEEFEKHVN
                     QIIYVSATPGPYEHEQTDTVIQQIIRPTGLLDPVIEVRPIMGQIDDLVGEINERVEKD
                     QRVFVTTLTKKMAEDLTDYFKELGIKVKYLHSDIKTLERTEIIRDLRLGEFDVLVGIN
                     LLREGLDVPEVSLVAILDADKEGFLRSERSLVQTIGRAARNEEGKVIMYADKVTDSMR
                     LAMDETSRRRTIQQKYNEEHGIVPKTIIKEIRDLISITKESEDDTKEAVQVSYEEMTK
                     EEKDTLLMKLEKEMKDAAKALDFETAANVRDMILELKASK"
     gene            12266..13768
                     /locus_tag="IXT20_000010"
     CDS             12266..13768
                     /locus_tag="IXT20_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294446.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAQ4858493.1"
                     /translation="MKRKHLFHSFVFLMTGLVTLAFGHVAQADNTTTAESSTSETMQK
                     IEPKKDVYVIASDSAFAPFEFQNAKGEYEGIDVKLMENIAKLQGFNIRMDYRGFSAAL
                     QALESGQADGMIAGMTVTDERKKSYDFSDSYFDSGIQLAVKKGDTSIKSYEDLKGKTV
                     GAKVGTESAEFLSDHESEYGYSIKYFDAADQLYDGLKVGAIDAMMDDYPVIGYAIKQG
                     QELETPIKKESGGKYAFAVKKGQNPELLNMFNEGLKELKRTGEYDKIIKEYVADGSET
                     SSESSTADESTFLGLIQNNYQALLKGLWRTILLTLISFALAIVIGVIFGLFSVAPIRA
                     LRTIASIYVDIIRGIPMMVLAFFIFFGLPGIVGFTIPDFLAGVITLTLNASAYIAEIV
                     RGGINAVPVGQMEASRSLGLSYNRTMQKIILPQAIRIMIPSFVNQFVISLKDTTIISA
                     IGVVELLQTGKIIVARTTQSTYVYLIIAIMYLILITALTKLAKVLEKKVK"
     gene            13770..14507
                     /locus_tag="IXT20_000011"
     CDS             13770..14507
                     /locus_tag="IXT20_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HAQ4858494.1"
                     /translation="MAEKILVEHLVKKYGDNTVLNDINVSINEGDVVCIIGPSGSGKS
                     TFLRCLNRLEEPSDGEIIIDGAHLMDKSTDINLVRQHIGMVFQHFNLFPHLSILENII
                     LAPMDLKKESREEAEKKAIKLLQTVGLEDKKDMYPEMLSGGQKQRVAIARALAMNPDI
                     MLFDEPTSALDPEMVGDVLNVMKKLAEQGMTMVIVTHEMGFAKEVANRVMFIDDGNFL
                     EDGTPQQIFENPQNERTKDFLNKVLNI"
     gene            complement(14552..14725)
                     /locus_tag="IXT20_000012"
     CDS             complement(14552..14725)
                     /locus_tag="IXT20_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HAQ4858495.1"
                     /translation="MEKKNQAQLSEGGIDYVLDIINEPMVLSYAMEQVERLLVCAGKQ
                     AMRLIRLGEKIRR"
     gene            14701..15363
                     /locus_tag="IXT20_000013"
     CDS             14701..15363
                     /locus_tag="IXT20_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar diacid utilization regulator"
                     /protein_id="HAQ4858496.1"
                     /translation="MELDFSSPYYVVVGQLSDNQQLCQERINPLLELLERVFSYYGQA
                     LLTVHRQQIIILVNRISRASLLSLLDKIQTKFNKMYQLQLNFGIGSLCYTEQETPQSF
                     LHAKQVCEWIAFHQSVNEIRFFEDLDLGIVLPAIPSDQRTLYVKRILKSLTEEVHLFK
                     KTLACFSKNNGSIKNCSEELFIHKNTLQYRLNKFHSLTGYTPRNYDDYHILKLAFLLV
                     QT"
     gene            complement(15445..16611)
                     /gene="dnaJ"
                     /locus_tag="IXT20_000014"
     CDS             complement(15445..16611)
                     /gene="dnaJ"
                     /locus_tag="IXT20_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010767443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HAQ4858497.1"
                     /translation="MATKRDYYEVLGLSKGASEDEIKKAYRKLSKKYHPDINKEPDAE
                     EKFKEVSEAYEILSDPQKRAAYDQYGHAGTDPNYGAGGAGGGFGGFGGFSGGGFGGFE
                     DIFDSFFGGGGRSVDPNAPRQGADLQYTVDLSFEEAIFGVEKEIKYNREEICHTCGGN
                     GAKPGTQPTTCHKCHGAGTINVERQTPLGRVMSRQTCDVCHGTGKEIKEPCPTCHGSG
                     HEKKAHSVKVNVPAGVEEGQQMRLAGQGEAGENGGPFGDLYVVFRVEESDIFDREGSE
                     IYYELPLSFVQAALGDEVQVPTVHGDVKLKIPAGTQTGTNFRLRGKGAPKLRGGATGD
                     QHVKVKLITPKNLNEQQREALRAFAEAGGIKVEEQQEDGFFDKFKDAFGGKKRK"
     gene            complement(16762..18591)
                     /gene="dnaK"
                     /locus_tag="IXT20_000015"
     CDS             complement(16762..18591)
                     /gene="dnaK"
                     /locus_tag="IXT20_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016635026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HAQ4858498.1"
                     /translation="MSKIIGIDLGTTNSAVAVLEGNEAKIIANPEGNRTTPSVVSFKN
                     GEIQVGEVAKRQAVTNPNTISSIKRHIGEAGYKVDVEGKSYTPQEISAMILQYIKGFA
                     EDYLGEKVEKAVITVPAYFNDAQRQATKDAGKIAGLEVERIVNEPTAAALAYGLDKTD
                     RDEKILVFDLGGGTFDVSILELGDGVFDVLSTAGDNHLGGDDFDNKIIDYMVAEFKKE
                     NSIDLSQDKMALQRLKDAAEKAKKDLSGVSSTQISLPFITAGESGPLHLEMTLTRAKF
                     DELTADLVERTKVPVRQALKDAGLSQSEIDEVILVGGSTRIPAVVEAVRKETGKEPNK
                     SVNPDEVVAMGAAIQGGVITGDVKDVVLLDVTPLSLGIETMGGVFTKLIDRNTTIPTS
                     KSQVFSTAADNQPAVDIHVLQGERPMAADNKTLGRFQLTDIPAAPRGIPQIEVTFDID
                     KNGIVNVSAKDLGTQKEQKITIKSSSGLTDEEIERMVKDAEANAEADKARKEEVDLRN
                     DVDALLFSVDKTLKELEGKVDEEEVKKAETARDELKAAVEANNIEEMKTKRDALNEIV
                     QNLTVKLYEQAAQQQAQENPEAAQGGADDVVDADFEEVDGDDK"
     gene            complement(18642..19205)
                     /gene="grpE"
                     /locus_tag="IXT20_000016"
     CDS             complement(18642..19205)
                     /gene="grpE"
                     /locus_tag="IXT20_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HAQ4858499.1"
                     /translation="MKENQEELDKEMTDAQPEPEIDVEAAEDSGISEAEAEEFETAKL
                     KAELEEMEDKYLRARAEIANMANRGKNEREQLQKYRSQDLAKKLLPSIDNLERALATE
                     VSDDQGAGLKKGVEMVLESLRNALEEEGIEKIPAKGEAFDPNLHQAVQTVPATEDTPA
                     DTIVEVLQEGYKLHDRVLRPTMVIVAQ"
     gene            complement(19299..20342)
                     /gene="hrcA"
                     /locus_tag="IXT20_000017"
     CDS             complement(19299..20342)
                     /gene="hrcA"
                     /locus_tag="IXT20_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HAQ4858500.1"
                     /translation="MLTKRQNDILRLIIQHYTSSGVPVGSKTLMSEGVKASSATIRND
                     MKALEDEGLLLKTHSSSGRIPSALGYRYYVDHLLKPARVAEDDLLLIRQSLGREFHEI
                     NEIIKQSAEILSELTSYTTFSLGPEIKDRRLTGFRIVPLNNRQIIAIVVTDMGNVESQ
                     VFTLPEYMGSQELEKMVQIVNDRLVGDPLMTVYHKLRTEIPMILHKYFQTTEGMSQLF
                     DTVLGHVFEDKVYVSGQMNLLDYEPRQDLDQFKSMFSFMKDSDGLTQMITPMDSNIHI
                     KIGAELGNDLLQNMSMIQASYEIDGHGRGTIALLGPTSMPYSRMFGLVDVYRRELAAK
                     LADYYRSLDLSNS"
     gene            complement(20576..21742)
                     /locus_tag="IXT20_000018"
     CDS             complement(20576..21742)
                     /locus_tag="IXT20_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HAQ4858501.1"
                     /translation="MIETINPAENKVSAYIHIPFCEHICYYCDFNKVFLEGQPVDEYV
                     EMLLNEMKIMLESHPTANLETLYVGGGTPTSLSAKQLDRLLSGAREILPFQNGNEFTV
                     EANPGDLTREKLQVMKNYGVNRLSMGVQTFDNRLLKKIGRKHTAEDVYQTMQFLEAEN
                     FTNVSIDLIYALPGQTLEGYLDTLEKALALDLPHYSLYSLILENKTMFMNWVRQGRLE
                     LPDQETETRMFEETIEAMEKHGRHQYEISNFGLSGHESQHNLMYWNNDHYFGFGAGAS
                     GYLGDIRYRNKGPIQHYLRPLRANERPVLEEEKLTRKNQIEEEMFLGLRKKTGITKKH
                     FYDRYQKTIESVYGDVLRELEQEGLLVNEEDRVYLTPRGTFLGNNVFERFLFEK"
     gene            complement(21758..21985)
                     /locus_tag="IXT20_000019"
     CDS             complement(21758..21985)
                     /locus_tag="IXT20_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HAQ4858502.1"
                     /translation="MKIAFHIFADFDLMPKVSFAITVYREAVKAPCSSDDNKQAFLEL
                     GFLDSKYAAYFNELVLERDFKKVENRHDFST"
     gene            22469..23059
                     /locus_tag="IXT20_000020"
     CDS             22469..23059
                     /locus_tag="IXT20_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HAQ4858503.1"
                     /translation="MKTAITFLLEEYADWEGAYITSTLNQNEKWQNKTASIQSEVTSI
                     GGLKTKIDYLIEQIPEKVDLFIMIGGNSWDIKNHHLYQIIDKLLKTDVPVAAICGAVD
                     YLAQSGLLQGYKHTGNAQFPWLESENYQNKEDFIEQQAVKDRNLITANGTAPIEFTDL
                     ILQAVHFETEEIREKTIRLYKLGFYDYYKKYGDPFS"
     gene            23249..23962
                     /locus_tag="IXT20_000021"
     CDS             23249..23962
                     /locus_tag="IXT20_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4858504.1"
                     /translation="MKKLGFILLLVSLFLVGCTSPSKENSDPSATTDKSSIRQTSSAE
                     KSSTQKSASTETNTSSTSSKVSEPNTSSTSSSTVETNISSSTIDTTSSQSSQSIDPLA
                     GYSDLQIEYARVWLAIRGNTFRDTSGDFELHVIHYPAGTPIAPYDAGSAVYPKDVVML
                     TGKYSYQGVLVYSSNHDGTITSYPVPSHWQMPADQTSYPAFIRETTQEIIDNASVVAI
                     PTGNPNDVKQLIDVTVIDN"
     gene            complement(24077..25087)
                     /locus_tag="IXT20_000022"
     CDS             complement(24077..25087)
                     /locus_tag="IXT20_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HAQ4858505.1"
                     /translation="MISYPLLERNATIGVTAPSSGVSEEHHSLLESALKRMEEKSFQV
                     VTGKTLWTQDKEKSGPARKRADEFNQMMQDENIQMIFPTWGGELLIEILDFVDFDALQ
                     PKWIVGYSDISTLLLAVTLKTGIATAHGTNLIDLRGEYLDETTAMWESVLKTREGESI
                     LQQSSEKYQKVWPHEHSSSSVFHLTEPTNWQSVSGDAVRMKGRLLGGCIDTIRHLIGT
                     PYGDVQSFKRKYNDGKPILWFLENCELSSVDLRRSLVQMKLAGWFDGSSGIMFGRSAA
                     EDLLDYKMKDVYEDLSKELKLPILYDIDCGHVPPQLTLINGAYAEVAVEDRKATVLQT
                     FI"
     gene            complement(25124..25606)
                     /locus_tag="IXT20_000023"
     CDS             complement(25124..25606)
                     /locus_tag="IXT20_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA editing protein"
                     /protein_id="HAQ4858506.1"
                     /translation="MIDLAKKFLHENIYFELYHHRAIYTNEDALVIKKEQGFTGTETK
                     SLFLKDKKDNNYVYLTFTTKQTDFKALGKIVGKRLSIVSSERMEEQTGQKQGAVSPFG
                     YEKQIPIIIDEELLSNEKLVFAPGRPDQTMVIQTVDLNKIIQLLEMPIFLMPANEKNN
                     "
     gene            25756..26124
                     /locus_tag="IXT20_000024"
     CDS             25756..26124
                     /locus_tag="IXT20_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chaperone"
                     /protein_id="HAQ4858507.1"
                     /translation="MEKFQPIINQIKEKEHQQKIKDLFAKITETYPQLDTTVKWNQPM
                     FTDHGTFIIAFSFAKNHFSIAPEKAAIRALEKNIQEAGYVYTDNVIKVPWKSVINWEL
                     IEQLISFNIEDKKGHDKFWR"
     gene            26589..27041
                     /locus_tag="IXT20_000025"
     CDS             26589..27041
                     /locus_tag="IXT20_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ4858508.1"
                     /translation="MKDFYSVNELAEQLGVTTRSIRNYLHEGKLKGTKVGGQWKFSER
                     NLFEFLYGDQAEEAAKEMQRFMLNAPITMRFNLQYRDFTAINQFREQLVQYHNDVYAN
                     KKDRLLQYDLYKDNHAEILIGGNFNYVVNFSQWINEKLLIQTDISLVS"
     gene            complement(27165..28016)
                     /locus_tag="IXT20_000026"
     CDS             complement(27165..28016)
                     /locus_tag="IXT20_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transporter"
                     /protein_id="HAQ4858509.1"
                     /translation="MGLLIALIPAVGWGIQPLILKKIGGRPTNEILGTGIGAVLVGLL
                     VQLFASPAGISVTTFLISLLSGAFWVIGQTGQYTSFNLIGVSKTMPISTALQLVGTSL
                     IGVFAFGEWSGSMGKIIGAAAIILLVFGSALTAISDDPGKKGGITKGISLLASTSIGY
                     WVYSALPKLVDASGLSIFFPQMIGVFLGAVIYVIFRKPTAFSEDKSWKATIVGAIFSV
                     SALAYIFSANENGVATAYIITQLNVVISTLGGMYILHENKTPKEKKLTIIGLLLIVVG
                     SMVTVFI"
     gene            complement(28029..28814)
                     /locus_tag="IXT20_000027"
     CDS             complement(28029..28814)
                     /locus_tag="IXT20_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAQ4858510.1"
                     /translation="MYKELENKVAVVTGGSKGIGTAIAQRFGKEKMNVVVNYNSDKEG
                     AEKAVKAIEEAGGKAVSVQADVSSEEGIQRLLDTALENFGTLDVWVNNAGMENQHPTH
                     ELSLEDWERVLKVNLTGVFIGTKAALNYFTENNKKGNIINMSSVHEQIPWPTFAHYAA
                     SKGGVKLFTETVAMEYAPKGIRINSIGPGAIRTPINAAKFDDPEQKELLESMIPLGRV
                     GEPKEIAATAAWLASDESSYVTGITLFADGGMTLYPSFQGGKG"
     gene            complement(29166..30107)
                     /gene="ribF"
                     /locus_tag="IXT20_000028"
     CDS             complement(29166..30107)
                     /gene="ribF"
                     /locus_tag="IXT20_000028"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin biosynthesis protein RibF"
                     /protein_id="HAQ4858511.1"
                     /translation="MNIIKIRHPYQSEQIPEDDVVLVLGFFDGVHIGHQKVIKTGMEI
                     AQKEGLKLALMTFNQHPSIVFKKIDPSSVKYLTSLKQKEEKMAALGIDYLYEVDFTSS
                     FAHLAPQEFVDQYIVGLHAKYAVSGFDYTYGPKDIADVAHFPEYAKGRFKIVTVSKEE
                     EQGEKISSTRIRGLLDSGQVKEAAKLLGCTYEVEGVVVHGDARGRLLGFPTANVKVKS
                     TVHLPKEGVYVSEIKIGETWYPAMGSIGHNDTFGEGRELTVELYILDFNQDIYGEHVA
                     VRWNQFLRDQVKFSSVEELIEQLKEDEQATAEYFSQQ"
     gene            complement(30111..31034)
                     /gene="truB"
                     /locus_tag="IXT20_000029"
     CDS             complement(30111..31034)
                     /gene="truB"
                     /locus_tag="IXT20_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HAQ4858512.1"
                     /translation="MDGLLPLWKERGMTSHDCVFKLRKILHTKKIGHGGTLDPDVDGV
                     LPICIGKATKVIEYLTDSGKIYVGEITLGFSTTTEDRSGERVETKPVDMSLSEKKIDE
                     VMASFIGEITQIPPMYSAVKVNGKRLYEYARNNETVERPVRKAQIYRFIRTSELTRDE
                     VTGTISWRFEVECGKGTYVRTLAVDTGKKLGYPAHMSDLTRTASAGMSQKDALTLEEV
                     AQAVKNGTIDDCLLPIEKGVERFKRVDITDEVWQKVKNGMRLDYTVFALPEMPSEEIA
                     LFYKGKVVSIYQPNPKEKNKLKPSKVLRNEE"
     gene            complement(31402..33764)
                     /locus_tag="IXT20_000030"
                     /pseudo
     CDS             complement(31402..33764)
                     /locus_tag="IXT20_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625383.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase family protein"
     gene            34212..34433
                     /locus_tag="IXT20_000031"
     CDS             34212..34433
                     /locus_tag="IXT20_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ4858513.1"
                     /translation="MIFLLTVNGPTNSNFYGAGSTDLLVTWLYLTVTKIDYNLASVIG
                     ILIFIYMQKFIYYRKKKVEFFTFLFLFFG"
     gene            complement(34541..34888)
                     /gene="rbfA"
                     /locus_tag="IXT20_000032"
     CDS             complement(34541..34888)
                     /gene="rbfA"
                     /locus_tag="IXT20_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HAQ4858514.1"
                     /translation="MANYRDRRVGQEILKEVNDILRKKVRDPRVENVTITDVKVTGDL
                     QQATIYYSILSDLASDKKTAQDGLNKASGLIRRELGHNLSIYKTPELFFELDESVVYG
                     NHIDELLRDLNKD"
     gene            complement(34915..37221)
                     /gene="infB"
                     /locus_tag="IXT20_000033"
     CDS             complement(34915..37221)
                     /gene="infB"
                     /locus_tag="IXT20_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HAQ4858515.1"
                     /translation="MGNKRIYELAKELKKSSKDVVEKAQQLGIDVKNHMGAITSNDEK
                     KLQQAFKNPSTKQQAAQQASRKPANQQPNDQRKKETTNNQQKNKNNRSYQDRGQGSGQ
                     VNQGKKPTTNQNNTGNTSSQNRQGTSQGGNQNRTNNNNKPNNNNRGKFNNNNRNRYNK
                     KGKKGKQQQSTKPAVPPRKFRELPDVLEYTEGMNVADIAKKIHREPAEIIKKLFMLGV
                     MVNQNQALDKDTIELLATDYGMEPQEKVQVDIADIDKFFEAEEVDPDKLVSRPPVVTI
                     MGHVDHGKTTLLDTLRHSRVTSGEAGGITQHIGAYQIDIDGKPITFLDTPGHAAFTSM
                     RARGASITDITILVVAADDGVMPQTVEAINHAKAAGVPIIVAVNKIDKPGANPQHVMQ
                     ELSEYELIPEAWGGETIFVEISAKFGQNIEELLEMILLVAEVEDLKADPTQRAIGTVI
                     EARLDKGKGPVSTLLVQEGTLHVGDPIVVGNTYGRVRVMTNDLGRRDKEAGPATPVEI
                     TGLNDVPQAGDRFVVFEDEKTARAAGEERAKRALLENRAATSRVTLDNLFESLKEGEL
                     KEVNVIIKADVQGSAEALAASLKKIDVEGVRVKIVHSAVGAINESDVTLAAASNAIII
                     GFNVRPTPQAKVQADTEEVDIRLHRIIYKAIEEIETAMKGMLDPEFEEKITGQMIVRE
                     TFKVSKVGTIAGAYVTEGYIRRDSGVRVIRDGIVIYEGQLASLKRFKDDVKEVKMGYE
                     CGAMIEKFNDIKVDDVIEGFIMEEVKND"
     gene            complement(37234..37545)
                     /locus_tag="IXT20_000034"
     CDS             complement(37234..37545)
                     /locus_tag="IXT20_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxQ-related RNA-binding protein"
                     /protein_id="HAQ4858516.1"
                     /translation="MNTVNRQKAMNLIGLAMRAGKLITGEELTIAEIRKQKAKLVFVA
                     TDASENTRKKIKDKSSYYEVPCFELFSEAEITQMIGKPRKVFGIMDAGFAKKTKELIE
                     G"
     gene            complement(37542..37835)
                     /locus_tag="IXT20_000035"
     CDS             complement(37542..37835)
                     /locus_tag="IXT20_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HAQ4858517.1"
                     /translation="MKQRKIPLRKSVVSGEMKPKKEMVRITRSKEGEVSIDPTGKMPG
                     RGAYVSLEPEEVQQAWDKKILDRVLETTLTDEFYQELLDYVTHQKARKELFGK"
     gene            complement(37857..39032)
                     /gene="nusA"
                     /locus_tag="IXT20_000036"
     CDS             complement(37857..39032)
                     /gene="nusA"
                     /locus_tag="IXT20_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HAQ4858518.1"
                     /translation="MSKEMLNALDALEAEKGISKEIVIDALEAALVSAYKRHYGQAQN
                     VEVEFDQKKGKIHVYAVKEVTEEVMDSQLEVSLKDALLINPAYEIGDTIRFEVTPKDF
                     GRIAAQTAKQVILQRVREAERTIIYNEFSAYEKDIMQGIVERQDKRYIYVNLGKIEAV
                     LSKQDQMPNEFYQPHDRIKVYVSRVENTSKGPQVFVSRSHPDLLRRLFEQEVPEVYDG
                     IVEIVSIAREAGDRSKVAVRSNDPNIDAVGTCVGPKGQRVQAIVNELKGENMDIVEWN
                     EDPAVFIANALNPAQVMDVIFDETNPKACTVVVPDYQLSLAIGKRGQNARLAAKLTNH
                     KIDIKSESDMTEFYENQEQQKETEELHDEAIVQSDLTDDEYETIAFNNETVEEKPEA"
     gene            complement(39056..39529)
                     /gene="rimP"
                     /locus_tag="IXT20_000037"
     CDS             complement(39056..39529)
                     /gene="rimP"
                     /locus_tag="IXT20_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HAQ4858519.1"
                     /translation="MSSVVETVTEMVTPILDEQNFELVEVEFVKEGKSWFLRVFIDKE
                     GGIDIEECAFVSEKLSEKLDAADPDPIPQAYFLEVSSPGAERPLKKESDYEKALGDYV
                     HVSLYQPVDGEKQYEGFLQSFDAEQLTLKIRIKTREKELTFDRKNIAKARLAIQF"
     gene            complement(39673..44025)
                     /locus_tag="IXT20_000038"
     CDS             complement(39673..44025)
                     /locus_tag="IXT20_000038"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HAQ4858520.1"
                     /translation="MSEKRDLFEKLLEQIQLEEAEKNHPLLTAGEMDSVVVHRKSRLW
                     EFTLHFSKILPIMLYRSLTQHLTIAFKDIAQVKLNILADDQTFDEQLLQDYWAQALEN
                     QQCDTPLAQQVLKTQVPVIKDRKVILPIDSTGAISYLKQQYLPLVEELFVSYGFPKFR
                     IEPEVDEQQAERVLKLFEERKQEQAEAFMKQAAESLVVHEQKKKERKEQSPALEGPIQ
                     LGRNIPADEPTTPMISIVEEERRVTLEGYVFDKEVRELRSKRKILTLKITDYTSSFIV
                     KKFSNGEKDEQVFDAISVGSWLKVRGSIQEDTFVRDLVMNAQDIIEVKHTPRKDYAPE
                     GEKRVELHVHSNMSTMDATNSISDLVAQAGKWGHRAIAITDHGGAQAFPEAHSAGKKA
                     GVKILYGVEANVVDDGVPIAYNDAHEALSEATYVVFDVETTGLSAVYDTIIELAAVKM
                     YKGNVIESFDEFIDPGHPLSRTTVDLTGITDEMVRGSKSEEEVLRMFLEFSKDTILVA
                     HNAAFDMGFLNTSYARYGIPEAANPVIDTLELARYLYPQFKRFGLGVLSKKFGVSLEQ
                     HHRAIYDAEATGHLAWIFVKEAMDNHNMLYHDQLNEHIGEGDSYKRARPFHVTILAKN
                     QAGLKDLFKLISMSNVEYFERVPRIPRSQLKKMRENLLIGSACDKGEIFEAMMQKGVE
                     EARNRAKFYDYIEVMPKAVYAPLIEQELVKNEHDLEEIIQNLVEIGKSLDKIVVATGN
                     VHYLNEEDAIYRKILINSMGGANPLNRHSLPDVHFRTTDEMLTAFHFLGEETAKEIVV
                     ENTNKIADICEEVIPVKDELYTPKIPGSEDEISELSYTKAKQMYGDPLPEIIQKRLEK
                     ELNSINGNGFSVIYLIAQKLVHKSNEDGYLVGSRGSVGSSFVATMTGITEVNPLAPHY
                     YCPECQYSEFFEDGTYGSGFDMPEKQCPKCGARLNKDGHDIPFETFLGFHGDKVPDID
                     LNFSGDYQAEAHNYTKVLFGEDYVYRAGTIGTVADKTAYGYVKGYERDNNLQFRSAEV
                     DRLAKGATGVKRTTGQHPGGIIVIPDYMDVYDFTPIQYPADDQNSEWKTTHFDFHSIH
                     DNVLKLDILGHDDPTVIRMLQDLSGIDPQTIPTDDPEVMRIFAGPEVLGVSQEQIYSK
                     TGTLGIPEFGTRFVRGMLEETHPTTFAELLQISGLSHGTDVWLGNAEELIRRGDATLA
                     EVIGCRDDIMVYLIHAGLDSGMAFKIMETVRKGQWNKIPDELRETYLSAMKENNVPDW
                     YIDSCSKIKYMFPKAHAAAYVLMALRVAYFKVYFPILYYCAYFSVRADDFDLVSMCKG
                     KDAVKQAMKEITDKGLDASVKEKNQLTVLELANEMLERGFKFGMIDLYKSDAVNFVIE
                     GDTLIAPFRAVPSLGTNVAKQIVEARKDGPFLSKEDLATRGKVSKTLIEYMNDNGVLK
                     DLPDENQLSLFDML"
     gene            complement(44237..45946)
                     /locus_tag="IXT20_000039"
     CDS             complement(44237..45946)
                     /locus_tag="IXT20_000039"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HAQ4858521.1"
                     /translation="MRQSKMLIPTLREVPNDAEVLSHQILLRAGYIRQVSAGIYSYLP
                     LANRVLEKLKRIMREEFEKIGAVEMLMPAILPAELWEESGRYETYGPNLYRFKDRNDR
                     KMILGPTHEETFTDLMRNEINSYKKLPLNLYQIQPKYRDEKRPRFGLLRGREFIMKDA
                     YSFHATEESLDETYKDYEKAYTEVFKRCGLEFRSIIGDGGAMGGKDSKEFMAISEIGE
                     DTICYSTESDYAANLEMATSYYVSKKSHETQLELEKVATPNVKSIDEVADFFSVEPQK
                     IIKSVLFIADEQPVLVLVRGDHEVNDVKLKNFLSADFLEEATEEEAQKYLGAEFGSVG
                     PVGVSDDVKVYADRYVQDLANAITGANETGFHYKNVNPKRDFTVLSYEDLRFVQEGDP
                     SPDNNGVLAFTRGIEIGHIFKIGTRYSESMGATVLDENGREKFVIMGCYGIGVSRLLS
                     AIVEQHSDEQGINWPKGIAPFDLHIVQMNLKDEYQTSLTNELEEAMTKAGYEVLVDDR
                     NERAGVKFADSDLIGCPIRITVGKKAVDGIVEVKIKKTGEMVEVRKEELANTLPILLN
                     QGE"
     gene            complement(46014..47282)
                     /gene="rseP"
                     /locus_tag="IXT20_000040"
     CDS             complement(46014..47282)
                     /gene="rseP"
                     /locus_tag="IXT20_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HAQ4858522.1"
                     /translation="MKTILTFIIVFGILVIVHEFGHFFFAKRSGILVREFAIGMGPKI
                     YGHQAKDGTTYTLRLLPIGGYVRMAGNGDDETEMAPGMPLSLLLNSDGIVEKINLSRK
                     IQLTNAIPMELSRYDLEDELTITGYVNGDETEVVTYPVDHDATIIENDGTEIRIAPKD
                     VQFQSAKLWQRMLTNFAGPMNNFILAIVLFIILAFMQGGVQVTNTNRVGEIMPNGAAA
                     EAGLKENDEVVSVDGKEIHSWNDLTTVITKNPGKTLDFKIEREGQVQSVDVTPKSVES
                     NGEKVGQLGIKAPMNTGFMDKIIGGTRQAFSGSLEIFKALGSLFTGFSLDKLGGPVMM
                     YQLSSEAANQGVTTVISLMALLSMNLGIVNLLPIPALDGGKLVLNIFEGIRGKPLSQE
                     KEGILTLAGFGFLMLLMVLVTWNDIQRFFF"
     gene            complement(47443..48243)
                     /locus_tag="IXT20_000041"
     CDS             complement(47443..48243)
                     /locus_tag="IXT20_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HAQ4858523.1"
                     /translation="MKQRVITAAVALVLFIPIIWIGGIAVELTAAILAVVGVYELFRM
                     KGLTLLSFEGVLSAVGAVLLVLPKERWFFFLPENVSTFMLFYLIVMILLGTSVISKNT
                     YTIDEAGFPVLVSLYVGIGFQNFVNARTESLAVLIFGLFIVWATDIGAYMVGRRYGQH
                     KLSPDISPNKTIEGALGGIGSAVVVAFLYLLLYPAKELFNHGMFVMLLMTIIFSAVGQ
                     FGDLVESAIKRHYDVKDSGNILPGHGGILDRFDSLLFVFPIMHLFGII"
     gene            complement(48240..49052)
                     /locus_tag="IXT20_000042"
     CDS             complement(48240..49052)
                     /locus_tag="IXT20_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HAQ4858524.1"
                     /translation="MLRFFPQKNKYIQEKSEFQFNTDGPIPQHIAIIMDGNGRWAQNR
                     RLPRVAGHKEGMETVKKVTKKASRLGVKVLTLYAFSTENWKRPKDEVSFLMQLPVDFF
                     DTFVPELIKENVKVHVMGYENVLPEHTQDAVRRAIEQTKNNTGMVLNFALNYGSRAEI
                     VTAVQEIAEEVAKGEIHAEEIDDELIAKHLMTGFLPKELQDPELMIRTSGEERISNFL
                     LWQIAYSELYFTKALWPDFDGAHLEEAIASYQNRDRRFGGVKKTEDEKGDQS"
     gene            complement(49247..49804)
                     /gene="frr"
                     /locus_tag="IXT20_000043"
     CDS             complement(49247..49804)
                     /gene="frr"
                     /locus_tag="IXT20_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HAQ4858525.1"
                     /translation="MADAIMTEAKEKMKKAAQNLQRELGQIRAGRANASLLDRITVNY
                     YGAPTPLNQMASIQIPEARVLMITPFDKSILQDVEKAIMASDIGISPTNDGNVIRLVI
                     PQLTEERRKELAKDVKKEAENAKIAIRNIRRDAIDEYKKQQKNGDITEDDLRGLEKDV
                     QTLTDNSIKEVDKIAADKEKELLEV"
     gene            complement(49807..50529)
                     /locus_tag="IXT20_000044"
     CDS             complement(49807..50529)
                     /locus_tag="IXT20_000044"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010761216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HAQ4858526.1"
                     /translation="MVTPKYQRVVLKLSGEALAGDAGFGIKPPVIKEIIQEIKEVHEL
                     GVEMAIVVGGGNIWRGQIGAQMGMERAQADYMGMLATVMNALALQDTLENVGVPTRVQ
                     TSIEMRQIAEPYIRRKAERHLEKGRIVIFAGGTGNPYFSTDTTAALRAAEIGADVILM
                     AKNNVDGVYSADPKVDANAVKFEELTHLEVIAKGLQVMDSTASSLSMDNDIPLLVFNL
                     NEHGNIRRAILGENIGTTVRGK"
     gene            complement(50665..51546)
                     /locus_tag="IXT20_000045"
     CDS             complement(50665..51546)
                     /locus_tag="IXT20_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HAQ4858527.1"
                     /translation="MADVTAKMVKELREMTGVGMMDAKKALVEVEGDMEKAVDLLREK
                     GMAKAAKKNDRIAAEGLASVAVKGNTAAIVEVNSETDFVSKNEMFQDLVKEIAELVAE
                     NKPADMEAAMKIKTDKGTIESDLIEATQVIGEKISFRRFEVVEKDDNAAFGGYLHMGG
                     RIAVLTVLDGTTDETVARDVAMHVAAINPRYVNESQIPEAELEHEKTVLTEQALNEGK
                     PANIVEKMVEGRLKKFKAEIALVDQPFVKDPDMTVEKYVASKGATVKTFVRFEVGEGI
                     EKREDNFVEEVMSQVKK"
     gene            complement(51645..52427)
                     /gene="rpsB"
                     /locus_tag="IXT20_000046"
     CDS             complement(51645..52427)
                     /gene="rpsB"
                     /locus_tag="IXT20_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HAQ4858528.1"
                     /translation="MAVISMKQLLEAGVHFGHQTRRWNPKMKKYIFTERNGIYIIDLQ
                     KTVKLVDAAYDYMKSVAEEGGVALFVGTKKQAQEAIKEEATRAGQYFVNHRWLGGTLT
                     NWDTIQKRISRLKQINAMEEDGTFEVLPKKEVAGLNKERERLEKFLGGIADMPRIPDV
                     MYIVDPRKERIAVQEAHKLNIPIVAMVDTNCDPDEIDVVIPSNDDAIRAVKLITSKMA
                     DAFIEGNQGEDQVVEEDFTEENTATSIEEIVDVVEGDNSSAE"
     gene            52786..53265
                     /locus_tag="IXT20_000047"
     CDS             52786..53265
                     /locus_tag="IXT20_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgR family transcriptional regulator"
                     /protein_id="HAQ4858529.1"
                     /translation="MRKAQRHLLIKQVIEEFPIRTQEELLNKLGHYGVTATQATISRD
                     IRDLKIVKAPDENGVSRFVLFREKGTPETRTEEEDRLVQMIEDIVLKVERVHFLTIVN
                     TLPDNAHLFASVLDEIKPPSIVSTIAGFDTIMVISENEEDAKRVEEFLHHPTEVSLF"
     gene            53482..54573
                     /gene="phnW"
                     /locus_tag="IXT20_000048"
     CDS             53482..54573
                     /gene="phnW"
                     /locus_tag="IXT20_000048"
                     /EC_number="2.6.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-aminoethylphosphonate--pyruvate transaminase"
                     /protein_id="HAQ4858530.1"
                     /translation="MYKLLTPGPLTTSETVKNAMLIDHCTWDQEYKEITQWIRKKLLD
                     LAQVSEGIYTTVLMQGSGSFGVESVLSSVIRPEDTVLICSNGAYGQRMINMCECLKLN
                     YTVYEVSEHQILQAHVITQLIEENPSISALAMVHSETTSGILNNLEEIAAVTQEKDLL
                     FIVDAMSSFGGIPIRVAELGIDFLISSSNKCIQGVPGFSFVLANKAELEKRKNVSRSL
                     SLDLYDQYRVMENENGKWRFTSPTHTVLAFQQALKELEAEGGIETRYSRYLANNHVLR
                     QKMTEMGFQPFIQEYQGPFITSFLYPKDWRFDFNHFYQYLKQNGFVIYPGKISDHECF
                     RIGNIGEIYPEDILELTAIIKDYKENVND"
     gene            54566..55351
                     /locus_tag="IXT20_000049"
     CDS             54566..55351
                     /locus_tag="IXT20_000049"
                     /EC_number="3.11.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HAQ4858531.1"
                     /translation="MIKTVIFDWAGTTVDFGCMAPVHAFRNAFLEKGIQLTDKEIREP
                     MGKLKWDHIQQLLSLPSVKKQWIAIYGQLPQEKEVEELYQQFEHYLFKDLVKHSTLKP
                     DTLETIERLRNAGINIGSTTGFTAPMMTIVAETAKKEGYSPDFMATSEDVEGYGRPFP
                     YMIHKNMQYFKNKSVDEVIKVGDTVSDIQEGKNAGVLTVGVIEGSSLMGISYDEYQKL
                     EMGKKDRLKQSVALRFQQAGADICVNNLKELTEYILYADKNPE"
     gene            complement(55697..57388)
                     /locus_tag="IXT20_000050"
     CDS             complement(55697..57388)
                     /locus_tag="IXT20_000050"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HAQ4858532.1"
                     /translation="MNNKDLVAKAVYDAVKDDLTLEQVEQLLENPKSAEHGDVAFPAF
                     SLAKVYRKAPQQIAADLAEKIDSANFEKIEVVGPYLNFFMNKELISKKVLQTVVKEKE
                     HYGDSNIGNQGTVPIDMSSPNIAKPISMGHLRSTVIGNSIGFIMEKIGYQPIRINHLG
                     DWGTQFGKLIVAYKKWGTEEAVKAEPINELLRLYVQFHEVAETEPELNEEARAWFKRL
                     EEGDEEAIQLWQWFRDESMKEFNKIYDLLEVRFDSLNGEAFYNDKMDEIVKLLEEKHL
                     LNEDKGAEIVDLSAYDLNPALIKKSDGATLYITRDLAAALYRKRTYDFKQSLYVVGNE
                     QSYHFKQLKAVLKEMGFDWSDDMRHIPFGLITQGGKKLSTRKGKIVLLEEVLNEAIQS
                     AKEQISEKNPDLENKDAVAKQVGVGAVIFHDLKNDRLNTFDFNLEEVVRFEGETGPYV
                     QYTHARAVSLLEKAGFVPSETADYALNDDTSWEVVKLVQKYPETVLSAGEKYEPSVIA
                     KHAIKLAQAFNKYYAHTKILADDEQKEARLALVYAVTVLLKEDLRLLGLHAPDKM"
     gene            complement(57810..58757)
                     /gene="arcC"
                     /locus_tag="IXT20_000051"
     CDS             complement(57810..58757)
                     /gene="arcC"
                     /locus_tag="IXT20_000051"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HAQ4858533.1"
                     /translation="MAKRKVVVALGGNAILSTDASAKAQQEALMETAKYLVKFIEQGD
                     ELIISHGNGPQVGNLLIQQQAADSEKTPAMPLDTCVAMTEGSIGYWLQNAMGEVLKEK
                     GIDKDVVSLVTQVIVDENDPSFKNPSKPVGPFYTEEEAKEQMNADSTVTFKEDAGRGW
                     RKVVASPKPISIKEARVIETLVDQGVITVSVGGGGIPVVETATGLEGREAVIDKDFAS
                     EKLAEIIDADLLIVLTGVDNVYVNYQKPDQKKLETVTVSEMKQYIDEKQFAPGSMLPK
                     VEAAIAFVEAKPNAKAIITSLENIENLLASEEGTIIVAD"
     gene            complement(58872..59891)
                     /gene="argF"
                     /locus_tag="IXT20_000052"
     CDS             complement(58872..59891)
                     /gene="argF"
                     /locus_tag="IXT20_000052"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003073325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HAQ4858534.1"
                     /translation="MKESVFQGRSLLAEKDFTKEELQYLIDFSEHLKDLKKRGIPHHY
                     LEGKNIALLFEKTSTRTRSAFTTAAIDLGAHPEYLGANDIQLGKKESTEDTAKVLGRM
                     FDGIEFRGFSQKMVEELAEFSGVPVWNGLTDEWHPTQMIADFLTIQENFGTVEGITVA
                     YCGDGRNNMANSLLVTGAILGANMRIVAPKELQPEEEIVKMAEGFAEKSGAQLMITDD
                     VDKGVDGADVLYSDVWVSMGEEDKFEERIKLLKPYQINMEMVEKTHNTDRLIFLHCLP
                     AFHDTNTVYGEQMKERFGITEMEVTDEVFRSKYARQFDQAENRMHSIKAIMAATLGNL
                     FIPRV"
     gene            complement(59982..61211)
                     /gene="arcA"
                     /locus_tag="IXT20_000053"
     CDS             complement(59982..61211)
                     /gene="arcA"
                     /locus_tag="IXT20_000053"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HAQ4858535.1"
                     /translation="MDKPIHVFSEIGKLKTVLLKRPGQEVENLTPDIMDRLLFDDIPY
                     LPIAQEEHDNFAKTLQNEGVETLYLEKLAAEAIDAGNVKEQFLNKMLDESHIASNAVR
                     EGLHEFLLSMETQEMVDKIMAGVRTKDIEVRSSSLYDLSADDDYPFYMDPMPNLYFTR
                     DPSASMGNGMTVNKMTFEARRRESMFTEYILKHHPRFANKGIEVWLDRENPDHIEGGD
                     ELILSDKVVAVGISQRTNAKALEKLARHLFAKNSGFEKVLAIKIPNNRAMMHLDTVFT
                     MVDHDKFTIHPAIQSKDGKMDVFTIEPDGDDIKITHSDNLHETMKAALGLDDLVLIPT
                     GNGDEIVAPREQWNDGSNTLAIAPGVVVTYNRNYVSNELLRSYGIKVLEINSSELSRG
                     RGGPRCMSQPLVREDLK"
     gene            61672..62373
                     /locus_tag="IXT20_000054"
     CDS             61672..62373
                     /locus_tag="IXT20_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HAQ4858536.1"
                     /translation="MRVNKIDFHFSKLRYQPDFEHFSDEEFQQIKEHTVLRSYKKGQI
                     LFDDGDPRQRLYILTKGLVRLERYDESATYYYYDYVNTNNLFPMSGMFGEETYTYTAQ
                     AMTDIETFYLPVSYYEKLARKNPEQLIYMIQKISKVVEIHELRIQIGLTSSAFDRVKQ
                     NLFILKEELGVPTKDGHITIPYPITLKELAVNSGTTRETAGQVIKQLRDSGLLDYDKK
                     RFTFCKTATTPAMRE"
     gene            complement(62945..63961)
                     /gene="tsaD"
                     /locus_tag="IXT20_000055"
     CDS             complement(62945..63961)
                     /gene="tsaD"
                     /locus_tag="IXT20_000055"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HAQ4858537.1"
                     /translation="MNEELILAIESSCDETSVAVVRNGTEILSNIVASQINSHKRFGG
                     VVPEVASRHHVEQITLCLEDALVEAGVSAEDLSAVAVTYGPGLVGSLLIGISAAKAFA
                     WAHQLPLIPVNHMAGHIYAARLVKPFQFPLMALLVSGGHTELVYMQEDGSYEIIGETR
                     DDAAGEAYDKVGRVLGLSYPSGKEIDQLAHQGKDNYHFPRAMIHEDNYDFSFSGLKSA
                     FINLVHNAQQRGEDLDKNDLAASFQASVIDVLITKTLRACQNYPVKQLIVAGGVAANQ
                     GLREGLQDALATKLPEVELVIPPLRLCGDNAAMIGAAAHVEMQKRNFASYQLNADPSL
                     VLSE"
     gene            complement(63958..64422)
                     /gene="rimI"
                     /locus_tag="IXT20_000056"
     CDS             complement(63958..64422)
                     /gene="rimI"
                     /locus_tag="IXT20_000056"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HAQ4858538.1"
                     /translation="MICRKKDFDEAYLAECVWMISENAYPHGSPWTKMQFLADIQQPH
                     SDYLLLVEDNQICGFIGYSKVIDEVEITNIAVAVSEQGKGHARHLLQLLITEQIKDSL
                     HVFLEVRLSNEAARKLYESEKFRILGKRKSYYRNPTEDAIIMSRKLKTETMK"
     gene            complement(64429..64971)
                     /gene="rimI"
                     /locus_tag="IXT20_000057"
     CDS             complement(64429..64971)
                     /gene="rimI"
                     /locus_tag="IXT20_000057"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HAQ4858539.1"
                     /translation="MLKKFRAYARSFFQDKMIYTEKTIEQIDRRYVLRELTTEDIKEL
                     LDVEREVYNGELPWTKSAFISELKSPLPHLYLGIFDNGRMIGFIGSRAFGLDCHITNI
                     AVRPAYQRKRIGSLLIDEIENFAIMNRCETMSLEVRMSNQDAQRLYRKLGFQATTIKK
                     RYYTETKEDALDMIKLLENE"
     gene            complement(64955..65779)
                     /gene="tsaB"
                     /locus_tag="IXT20_000058"
     CDS             complement(64955..65779)
                     /gene="tsaB"
                     /locus_tag="IXT20_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HAQ4858540.1"
                     /translation="MYHCTSFAFGIFILRIKKYATMEGENVIAERIKYMITLGIDTAN
                     QTLAIGVVEDEQILGQIQTNIKRNHSVTLMPAIDQLFADLQISPKDIDRIAVSDGPGS
                     YTGLRIGVTTAKTIAYTLDKELVGVSSLKTIAANCIGVDKIIVPMFDARRKNVYAGAY
                     RWKENRLEIVLEDVHISASDLFTQLQKLNEAIYFVGSDCHKFFYEIKEILPSAMINTV
                     PAWDIPSGVTVAQLGALAEPVAEKQAFLPRYLKRVEAEEKWLETHTPGDESYVEKI"
     gene            complement(65868..66848)
                     /locus_tag="IXT20_000059"
     CDS             complement(65868..66848)
                     /locus_tag="IXT20_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAQ4858541.1"
                     /translation="MATIKDIAQLAGVSPATVSRVLNYDPDLSVGLATKQKIFEAAER
                     LNYTKHRKNAANTKQTLRLIQWYDDTEELEDLYYLSIRLGIEKKAEEANVTLLKETWD
                     SISDLPTDGTIALGKFDEAQLLEMKGSQNALLLVDSDGTSQGIDSLVVDFKSSVQKVI
                     EHFVSHKAQHIAMLAGTEYTKKNHQRLKDPRIMAFQQVLAEKGYDEGPIIEADFSVES
                     GYQAVKQYLKTNPHVPDALFAANDALAIGALKAIQEKGLVVPEDISVMGFNDVSVAKY
                     VTPSLSTVKVYTEWMGELAVETILQLSNSKAPVSRKIIVETELIIRESTK"
     gene            complement(66872..68356)
                     /gene="galT"
                     /locus_tag="IXT20_000060"
     CDS             complement(66872..68356)
                     /gene="galT"
                     /locus_tag="IXT20_000060"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HAQ4858542.1"
                     /translation="MSISQTICDFATLAIEAGGWMEMDRLYLQNRLLGMVGEDALESI
                     EVRPVKEESVDLLDQLVAKARENGVLSELPAEQEIFEAQLMDFLTPPPSVVNAFFAQH
                     YSKSPEEATEYFFNLSKKNDYIKTRAIAKNIKFPAESEYGELEITINLSKPEKDPKQI
                     AAEREAVKVDYPTCMLCMENEGYRGRLNYPARTNHRIIRMNLDGESWGFQYSPYAYYN
                     EHSIILSEQHRPMKITKETFARLLRITEVLPHYFVGSNADLPIVGGSILSHDHYQAGR
                     HEFPMAKAPIEKLIKLADYPQVIAGVVKWPMSVIRLQSTEKKLLIEAANSVLEKWKAY
                     SDETVSIRAFSADGTPHHTITPIARRRDNYFELDLVLRDNNVSEEHPDGIFHPHKDVQ
                     HIKKENIGLIEVMGLAVLPPRLKPELVEVEHFLLGEENQMAEYHRPWAEQLKKEHPDL
                     TKDTVTDVVQKSVGQVFARVLEDAGVFKRDEKGQAAFERFTAIL"
     gene            complement(68368..69357)
                     /gene="galE"
                     /locus_tag="IXT20_000061"
     CDS             complement(68368..69357)
                     /gene="galE"
                     /locus_tag="IXT20_000061"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010767298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HAQ4858543.1"
                     /translation="MTILVLGGAGYIGSHAVDQLVQKGYQVAVVDNLLTGHKQAVHPD
                     AHFYEGDIRDKEFLRSVFEKEPIEGVIHFAASSLVGESVEKPLMYFNNNVYGMQILLE
                     VMHEFNVNKIVFSSTAATYGEPKESPITEDTPANPKNPYGESKLMMEKMMKWCDQAYG
                     MRYVALRYFNVAGAKADASIGEDHTPETHLVPIILQVALGQRKALAVYGDDYDTPDGT
                     CIRDYVQVEDLIAAHILALEYLKEGNESNFFNLGSSKGYSVKEMLEAAREVTGKEIPA
                     EIAPRRAGDPSRLVASSEKAREVLGWKPEYTDIKAIIKTAWDWHVSHPNGYEE"
     gene            69606..69773
                     /locus_tag="IXT20_000062"
     CDS             69606..69773
                     /locus_tag="IXT20_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3042 family protein"
                     /protein_id="HAQ4858544.1"
                     /translation="MKKFVSGILVGSLATAAAVAGLVASVKKTVIDPIDEKEAMIEEN
                     RKKAMRKRVSR"
     gene            complement(69835..71646)
                     /locus_tag="IXT20_000063"
     CDS             complement(69835..71646)
                     /locus_tag="IXT20_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HAQ4858545.1"
                     /translation="MKIGIIGAGPRGLSIAERILHNNQDRHSLELFLFDPNGPGGRVW
                     RLNQPPELLMNSVSQQVTLFTDETLSSGGLVTQGPNLFQWSKGPATDYIEQTVTKNKQ
                     LIIEEINRLEKDQQSTRCLYGLYQRWFFSKLQEEFPASIQFVYSLVKKVSKRKDGYLL
                     QTDQQSYSIDQLVLASGHWENDETEEERVWVDYAKKNDLLYQLPSNPADVDLSKIPPK
                     ENVFLRGLGLSFFDYVGLFTVSREGRFENEKGKLVYHPSGKEPIVYSGSRRGLPYYPR
                     GRNQKQGGSMAWPRLLTKENLEQWHQSGFLTGETFFDYLKKDVELFYYKKIIEEYHLP
                     IPKKNFEHDFLSLSSEECLGKHPALIPYRWDWSYLEIPLTYQDESFAEASRAFIEGQI
                     KEAEKGNCTGAFASTFDALKDWRDPIRQAIEWHVFSAKEYKEILWGWFTHLNSFLTIG
                     PPVIRTKELAALIDAGIFHLFEPPFDIQQKHNRYVISLQDKEISSRYLIEARLPQTSF
                     SRSSNPVIKNLKENKLVRPFSFSDNTGSYHSEAIDVERGTNRVIDASGEIQSDFYCYG
                     IPVEGVDWLTAAVARPYTDPWNLRQADKIAQLILTDK"
     gene            complement(71643..72008)
                     /locus_tag="IXT20_000064"
     CDS             complement(71643..72008)
                     /locus_tag="IXT20_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 family protein"
                     /protein_id="HAQ4858546.1"
                     /translation="MIKLKRVYLDAEKEDGYRILVDRFWPRGISKEKAQLDLWLKEIA
                     PSNEARKTFDHVPERFASFAKKYQQELIFEEEKKQAIFKLSQLMKEHPVITFVYGAKN
                     EHQNHAVLLKKFIEMEACK"
     gene            complement(72171..72566)
                     /locus_tag="IXT20_000065"
     CDS             complement(72171..72566)
                     /locus_tag="IXT20_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HAQ4858547.1"
                     /translation="MVLWVINIILLLIVLAIIFWEVYLRVMAKRSATTLTEEEFREGM
                     RKAQVIDVREKDVFDAGHILGARNIPYTVLKDSLGSIRKDQPVYIYDQKKSLSIRTAN
                     KLRKAGYQEIYLLKGGYDGWSGKIKSKKV"
     gene            complement(72584..73546)
                     /locus_tag="IXT20_000066"
     CDS             complement(72584..73546)
                     /locus_tag="IXT20_000066"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HAQ4858548.1"
                     /translation="MDKKIIGIDLGGTTAKFAILTPEGEIQQKWSIDTNILDDGKHIV
                     PEIIESINHRLNLYNMKAEDFIGIGMGTPGSVDSEAGTVIGAYNLNWTEVQFVKKLIE
                     AGTGIKFAIDNDANVAALGERWKGAGENDPDVVFVTLGTGVGGGIVAGGNLIHGVSGA
                     GGEIGHITVDPEGFECTCGKRGCLETVSSATGVVRLGRYLAEEYAGDSKLKAMLDNGE
                     EVTSKDIFEMAQEDDPFALMVVDRVCFYLGLACANLGNTLNPSSIVLGGGVSAAGEFL
                     RSRVEKYFKEFTFPQVRESTKIKLAELGNEAGVIGAASLALKFA"
     gene            complement(73546..73758)
                     /locus_tag="IXT20_000067"
     CDS             complement(73546..73758)
                     /locus_tag="IXT20_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgQ family protein"
                     /protein_id="HAQ4858549.1"
                     /translation="MESLYDVQQLFKKFGIFIYVGARIYDIELMTIELRNLYDSRLID
                     HDTYMTAWRILKREHRIEESRKKGNL"
     gene            complement(73779..74477)
                     /locus_tag="IXT20_000068"
     CDS             complement(73779..74477)
                     /locus_tag="IXT20_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HAQ4858550.1"
                     /translation="MNYQQQIKLRAWLRRPLWTYAFLGIQTAVFLMMELFPRLEIPYY
                     AGMYGPYLVHFNEWWRLVTPIFIHFGLMHFVMNSLILYFMGQQIEAIYGHWRFFLIYM
                     FSGIMGNTASFAFNEANVLSGGASTSIFGLFGALFILGFHFKHNPAIQQLVRHFLLFI
                     VLTFVFGLLDTSVDVWGHVGGLIGGLVLGNVLGLPKQQTSYSIHQRILSTLVFVFLFV
                     ICILLGLKKYGLLV"
     gene            complement(74497..75039)
                     /locus_tag="IXT20_000069"
     CDS             complement(74497..75039)
                     /locus_tag="IXT20_000069"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HAQ4858551.1"
                     /translation="MDQLKVRLRRKGIEKLKNLAKSGKKKEKEQRIYKQLFASEYWKS
                     AKVIGTTLSNEFELDTQPVIQRAEKEQKKIVVPKTLPKRQMAFYEMDEQSVLVRSSFG
                     VLEPQSEKLYLPEMIDLLIVPGIIYNRAGYRIGFGGGYYDRYLNNFHKKTCSLVFREQ
                     LNEQWQPESFDQKIQQLFID"
     gene            complement(75171..76175)
                     /locus_tag="IXT20_000070"
     CDS             complement(75171..76175)
                     /locus_tag="IXT20_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HAQ4858552.1"
                     /translation="MKKIYWTFYFLLFVLVILSIGSLMIGTPFIYLDQLFATFIGKES
                     SSLALIVREFRVPRLLISLLAGACLGVSGYILQGVTRNELADSSILGINAGAGFLVML
                     YLGFFSHYSLPFLLLVVAFLGGVLAACCVYLAAYDRNGFLGMNRILLSGIAVNAGLSA
                     LTLLCTIQLSKENYGFVNAWLAGSIWGASWPYVWALLPWAVILIPLGGLYAQRIGLLS
                     FGQERAQGLGLNVAKSQKILLLLAVALASGSVAVTGSLSFVGLLAPHAAKLVVRKENN
                     WTFVLSGLFGSIFLLSADMVARVILPSGEVPTGILIALFGAPYFLYALFIRQKHALNS
                     "
     gene            complement(76172..77161)
                     /locus_tag="IXT20_000071"
     CDS             complement(76172..77161)
                     /locus_tag="IXT20_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HAQ4858553.1"
                     /translation="MRTMKKNRLLLLVCFVLLIIFFVALSNGAATVGKADFWQALFDF
                     DRSKQSHQIIRNIRLPRVAGAFLVGSCFALSGALMQGVTRNPLADSGLLGINAGASLA
                     MALSFAFWPKASDFSVFLMSLAGSFAVTLLVFGFLQFSKMGMNPLQLILAGVAISSFF
                     TAISQALTQFFNLQQDLAFWFVGGAANITWKQLAVLFPLYILAVLVSFSLGKAISLIH
                     LSETHALALGSHPKRTRLIVFCLVAVLAAIAVSLVGPVSFIGLLVPPVVRGMVGTDYR
                     YVLPVSFLVGGGLVMLADFTARMINPPFETPFGLVIALIGVPFLLIQVRREQR"
     gene            complement(77158..77964)
                     /locus_tag="IXT20_000072"
     CDS             complement(77158..77964)
                     /locus_tag="IXT20_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ4858554.1"
                     /translation="MTRLRAADIAAGYENKKIIENMSVTIPEGKITSLIGPNGSGKST
                     LLKSFTRILPVKSGKIIVNNSDISTYSSKELAKKIALLAQTSEHPTGMTVEEVVSYGR
                     YPYQKLFSGMDTQDHEAIEWALDATGLAGFRMRDIATLSGGQTQRVWIAMALAQETDI
                     LILDEPTTFLDPAHQLEILQLLTEINQMKQTTILMSIHDINHASRFSDYLLAMKNGKL
                     ITEGTPEEVITKECMKKIFEIDATIVTLPRTNKPVFLTYDLLPKEQEDQL"
     gene            78130..79086
                     /locus_tag="IXT20_000073"
     CDS             78130..79086
                     /locus_tag="IXT20_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAQ4858555.1"
                     /translation="MKLKTKVFTLSSILLLAGVLSACSPSSDKETATTANSTTSSNST
                     HTVTDTLGHKVEIPNAPKRIIGSYLEDYLIALGEKPIAQWTVGSGSIQDYLQDDLKDV
                     PTISYDLPYEKVLSFEPDLLLISSSATVEGGKYEQYSKIAPTYVVKNGTDVTWEEQLK
                     DVGKALAKEKEAEKIISDYQKSIKKTREELADKIENKTAAVLWVTNNSAFMVSKNRSS
                     GRIVYDDLEFGVPDLVEEVSKKATSDWSAVSSEKLAELDADYIILVNSDKDAAMFNEP
                     SWKNLKAVTDNHVLQFGPESSWLYNGPIASQEMVNDIKEKLN"
     gene            complement(79163..79681)
                     /locus_tag="IXT20_000074"
     CDS             complement(79163..79681)
                     /locus_tag="IXT20_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAQ4858556.1"
                     /translation="MFISMWAQDKNGLIGKNGLLPWRLPNDMRFFREHTMDRILVMGR
                     KTYEGMGDLSLPYRHIIVLTTQPNFRTKENAEVMHSIDELLSYSQTISEDIYVSGGSR
                     IFQELLPYTGRIWRTLIDSTFEGDTYIGNIDFSDFALIEEYEGITDQENLYAHRFQKW
                     ERVKNRVASKEE"
     gene            complement(79769..79918)
                     /gene="rpmG"
                     /locus_tag="IXT20_000075"
     CDS             complement(79769..79918)
                     /gene="rpmG"
                     /locus_tag="IXT20_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HAQ4858557.1"
                     /translation="MRVNITLECTSCKERNYLTSKNKRNNPDRLEKQKYCPRERKVTL
                     HRETK"
     gene            80146..80592
                     /locus_tag="IXT20_000076"
     CDS             80146..80592
                     /locus_tag="IXT20_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HAQ4858558.1"
                     /translation="MPWNMKDFPASMKNLDKLTRKKAIDIANALLDEGYPDNRAIPIA
                     IDQAKKWYEDASESEKRTFEKEKNPSKTDEHDTNPRAGKLLDSDVIVEYEEEQWIVKS
                     KGAKKASNHFDTKKEAIEKGKQVAQNKESTLFIYKKDGTKEKEIDY"
     gene            complement(80787..82682)
                     /gene="asnB"
                     /locus_tag="IXT20_000077"
     CDS             complement(80787..82682)
                     /gene="asnB"
                     /locus_tag="IXT20_000077"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HAQ4858559.1"
                     /translation="MCGIVGFVNDKDNKKTIINTMMDRIVHRGPNSSGEYIDKHVALG
                     FRRLSIIDLEGGTQPIYNEDRTKIIIFNGEIYNYQPLREELIAAGHVFQTHADTEVLL
                     HGYEEWGTELLQKIRGMFAFAIWDNEKNELFGARDHFGIKPYYYAEMNGTFMFGSEIK
                     SFLPHPDFNKELNKEALKPYMTFQYSPLNGETFFKDVYRLPEGHYYTYKDGKIDIQQY
                     WDADFETKETHSRQEWIEKIDETVQASIEAHTVSDVEVGSFLSSGVDSSYVTSVLKPD
                     HSFSIGFDDKTYNEAIEARKLTELLDLDNTAAVIDGDMSFKAFPLIQYHLDEPDSNPS
                     CVPLYFLANLASQSVRVVQSGEGADELFAGYQTYGFHTNSKFIRVIAQGLKKLPKGTR
                     YNLGRKIGKMKNFHGRIHLYESLAPAKEYFIGHARVFEESEASEVLTPKYQTAPSVDE
                     IMTVHYEKTEGIKDEVNKMQYVDLHQWMPKDILLKADKLSMASSLEVRVPLLDIEVMK
                     LAQQIPSKFLLNQNNTKDIFRQAANKHLPEEWSNRVKLGFPVPIKAWLKEEHGYEQVK
                     ALFEADFAKEFFDQEKIMKLLDDHHEGRKEEQRKIWTIFSFLTWYKVYFVDETIPQAE
                     AIDYVTV"
     gene            complement(83007..83981)
                     /locus_tag="IXT20_000078"
     CDS             complement(83007..83981)
                     /locus_tag="IXT20_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase family protein"
                     /protein_id="HAQ4858560.1"
                     /translation="MCTAITYATKDHYFGRNFDYEMSYNEVVTITPRNYRFDFRKVKN
                     LDKHYAMIGIAAGVSNYPLYYEATNEKGLSMAGLNFPGNADYKELQEGKDNVAPFEFI
                     PWILGQCSTIDEAKELLATINLVNIDFSEKLPLSPLHWLLADKEKSIVIESMKDGLHL
                     YDNPVGVLTNNPPFDYQLFNLNNYRSLSNGTPENHFSNQISLNVYSRGMGGLGLPGDL
                     SSVSRFVKATFTKMNAASGDSESESISQFFHILGSVEQQKGVCDTGERKYEYTIYSSC
                     CNVDKGIYYYRTYEDSQITAVNMNKEDLDRHELVSYPVIKEQQINYVN"
     gene            complement(84493..85269)
                     /locus_tag="IXT20_000079"
     CDS             complement(84493..85269)
                     /locus_tag="IXT20_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008377772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HAQ4858561.1"
                     /translation="MSKESMIKWMQDRLGKVTYSMTHRLGPNSYDCSSAVFFSMIAGG
                     FLPSGSMGNTETLFGMVGTKLKKISRSEVQRGDIFISGTPGGSNGSAGHTGIFLSNSS
                     FIHCSYTHNGIAVDTNDAYMSTKLQHNFYRILDGGTANTDNKPQMIQLALDGQFGNAT
                     ARRLQEYFDTAGKDGVISHQFKQTFNQNIYAAQFDNTLTGSNVIRALQGFLGIAQDGL
                     CGQNTIKALQRYLGTTQDGVISPVSNAVKELQRRLNANKL"
     gene            complement(85272..85676)
                     /locus_tag="IXT20_000080"
     CDS             complement(85272..85676)
                     /locus_tag="IXT20_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HAQ4858562.1"
                     /translation="MVIIDNLVLLSEFKNLVSNVYIQIFVWIVIADIITGVCKGLAGK
                     ETNSTKGLMGVVKHLLVVALVLIAYPYLKIMNFEGVATAFVLSYIAVYGISVIENLGQ
                     LGIPIPDFVKDRFSKLKDSTEKQGKNKKEGGQ"
     gene            complement(85977..86111)
                     /locus_tag="IXT20_000081"
     CDS             complement(85977..86111)
                     /locus_tag="IXT20_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HAQ4858563.1"
                     /translation="MYSYDDIKMMYDWNCFTADQVRQFVPLCITDEEAQKIINKTKVL
                     "
     gene            complement(86113..86520)
                     /locus_tag="IXT20_000082"
     CDS             complement(86113..86520)
                     /locus_tag="IXT20_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4858564.1"
                     /translation="MKTIYKVLYPMGYEEHQVEDNFPTSLPFVEVPPILFEKKEDETD
                     EGLGRRQQSQFFNFTENKWEEAVTQDYSKKLELLENLSIGLQVDNAALKKANEELTTK
                     AESLAQINSKTMLTSLQNTKDIATIKEQLDGGK"
     gene            complement(86548..87072)
                     /locus_tag="IXT20_000083"
     CDS             complement(86548..87072)
                     /locus_tag="IXT20_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4858565.1"
                     /translation="MADIVQLKEDGVAKYLKTHAKGIDGVEGVLVKATGNETVLGTKN
                     FKDGLQFNGLPVQAGMIERAITLADRSDTTNVTDVNGKIIRIGNIVFLTLNFKCGTWP
                     EGSETRWILKIPDGFKRDQGYPAQTALSLVRNASQPADARAFIDQSSIIQAKSGSGSS
                     YISGMWITQDPWPA"
     gene            complement(87072..87440)
                     /locus_tag="IXT20_000084"
     CDS             complement(87072..87440)
                     /locus_tag="IXT20_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4858566.1"
                     /translation="MTDIVKVKQNDVQVYPQTHWDAVEGKPETIKGDKGDPGQAATIT
                     VGTVTSGTTASVTNAGTASAAKFNFVLPKGDKGDKGDPGANATTTAVATTTANGLMSK
                     EDKVKLDGLANITFEKVGTV"
     gene            complement(87445..88233)
                     /locus_tag="IXT20_000085"
     CDS             complement(87445..88233)
                     /locus_tag="IXT20_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4858567.1"
                     /translation="MAKTKRMMETDEKTGVQRQFFPITHASAVLGLEEIIAGEATVLS
                     VNGKIGAVVITKEDLGLENVLTELPYASEEDDGIITSEMYQKILNSGEGDYVLPVATI
                     DRLGGIKIGELLTIDETGKVSAVRQSDVNFSLELKEKLDSLKNYTAGENITIDEDGKI
                     NAEVTGFYVLPTASEFVKGGIRVGEGLTMTDDVLSADKQFNYTAGANISISNTGVISA
                     TGGGEGGGVSQEYVDQKMSEAYQNAQAYTDSKIPNVSFEKVGEV"
     gene            complement(88233..89141)
                     /locus_tag="IXT20_000086"
     CDS             complement(88233..89141)
                     /locus_tag="IXT20_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate upper protein"
                     /protein_id="HAQ4858568.1"
                     /translation="MAKWNVVLSTTEPYNYVGMIQVRQGNKNSEVMEATITENGMPYD
                     LTGCKVYFESVVGDKYPVQLGTKVIDAKKGKIQYTFDQYSMQCLHRQTADFIIYKDDE
                     LIATTQDFSYFVIKAVSKTEGEMGSYWQTVEDLIADMSAFINENKGDFTDWMNARKKE
                     FEQWRQDQQNTFEAWREGQETDYLKWFESIKDILKSIDPGGVMLAELMDARVDIQGVR
                     HASISERLLADMDYLYQKLRATLFTIEYGEIEVTDILQDDLFSDNHEVEKVGTVEFPI
                     EEGALIIATVDDPKQNVFTLEKVGVI"
     gene            complement(89107..89625)
                     /locus_tag="IXT20_000087"
     CDS             complement(89107..89625)
                     /locus_tag="IXT20_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HAQ4858569.1"
                     /translation="MIEEKGLNHLKSLLNQPIGNHQCYALSAEYAGVMIGPDMGAGTQ
                     YEIKVRHGNVFSAAEIGRAYPWALYLWTVIVHPEYEQLVVGSIINWERNAKISDTFKS
                     HEYYGHTGVIKGLENGRIQTYEQNAESGGIVAEYDREFFGSGQIASICIPPDFEKGVV
                     INGKVERSTQHN"
     gene            complement(89636..92458)
                     /locus_tag="IXT20_000088"
     CDS             complement(89636..92458)
                     /locus_tag="IXT20_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4858570.1"
                     /translation="MTQEFIYAYKKMPDDLSVNGASLVDWEDLPEINRVLNGQYRFYG
                     NYSRSGQYRSYLKKGNFIKAKVPDGSWQYFEIYNIKKNLTSVSVTARHIGFMANKNFI
                     VKSFTDNGNGSQIMTNLKNSLAFDQKFNYLSNVGTTHQFTARQVAPVEAIIGSNNGNQ
                     NLTGVTSAELDMDNYDLKLVKQIGSDNGFRIDFGINLEAIEEEIDEESIVNSLYLVGG
                     VPDNDYDEDKEPIEYGYLEIDGVTNENRRIAKRENGDCKTVDELIKWGKTLFDNDRIH
                     EPKATHTVSMVALEHTLEYGEMYQELASLHFGDVAHVRAKELDIEIKERMVEYTYFPT
                     LGKYKDIVLGNDLSLYTSAVNTQAQELKKKIDNRTETLVQNVLNATAWITGNSGGHVV
                     FRPEKAPSEILIMDTPNVANAKRVWRWNLNGLGYSDNGVNGPFGIAMTSKGEIVADFI
                     KVGIINAEVLESSINDLGDVLKIVKGTLQIWNDNKKIMELTKKGMEFWNTKESIGTIG
                     TTDSAGNPFPGASTPTPLEDNSLVIRTNGDGKYILISPKEGKGLVMLGNGTSILYGSL
                     NLQEKLNAFGDVEFMKNVNIRGKLTINGQEVFPGQGGSGNNGGGSWNGMYPPEVTSQA
                     DKFAWELWVMLLSRGYSKASIAGILGNVQGEAGAAMNPDIAQVGGPAYGIVQWDGSAY
                     PLVPPATWDGRTYVQNLMRAAGITEDYRTMSAQGKLLDWTMYNGQWLGIVQPASVSGF
                     KAMTDPAAAAYTFERNYERPATTHPERQGWAVNWYNKFKDLQVPSAGSILSTAKSLMG
                     YFHYSQPLRWNFGSVENPDRNGYADCSSFVWLALTKAGYKTATRGTLWYTGSMAADAR
                     GARQYLTEISPNEAKAGDIIIVNLGAGAGNDGHTAILAEDWKGYSTSIVEMGGMNSNG
                     VGIGRVDWSFGYLLNGGDVCLARAKK"
     gene            complement(92455..93159)
                     /locus_tag="IXT20_000089"
     CDS             complement(92455..93159)
                     /locus_tag="IXT20_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAQ4858571.1"
                     /translation="MKPDEPNFIWKNRNAVIDMDCIIEGELPDIMPNKRYETYTIQGR
                     SGELTETFDDYEPFDLEIERITIPHSKLREVKRWLTGKSQLITHNDPDKYLDAICSMD
                     KEVPFENEWGFFYTFDVTFRCQPLKRKIGEAPKIFHSSVMDIFDHGDEIAHPYLEIES
                     NGGDITITVGNKSLTILNTLAATIVVDTQLGKAIQEGLNLFTRGDWPVLQPEWNQVKI
                     SGKFKEVRFWNRSVYL"
     gene            complement(93171..95480)
                     /locus_tag="IXT20_000090"
     CDS             complement(93171..95480)
                     /locus_tag="IXT20_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HAQ4858572.1"
                     /translation="MAGKESDVVLNFKTNGEVSYSKTIKEINKEMNLAAAEYKNQVSA
                     MDKDATQTEKLRAAKQKLEKQLGLAEQRSQMLREEYEKSVKETGEYSAESEKLYKQLL
                     NSETGENKLRTALEQTNDALKEQGDVSVDTAKKLQKIEETGEKIKGVGEKMSVGVTAP
                     IVAAGAAGLAAFGEVDEALDTIITKTGATGEQADRLSQSFKNVGSNTHLPLQTVGEAI
                     GEVNTQFGFMDKKLEDSTNYLLQYAEINDTDVSQSAISARQAIDAYGLEYEDLNSVLD
                     VTTKTSQNTGQSVDDLMKKAIDGAPQIKQLGLSFGEGVTLLGQFEQSGVDSSAALSSL
                     SKATVAYAKDGKSLSQGLGELQDKVKNAGSETEAINAAAEVFGTKGGPRMADAIRRGT
                     LNLEDLAKTAGESGGAVGDTFDATLDPIDQADQAMNNAKLAMADVGEAVQVSLLPFFE
                     KATSLLQEFSKWWGSLDQDTKNWIITIAGIAAAVGPVLIVLGSLMGSVTKIVGGVKSF
                     IGVWQGLAGLFGMSGGWFALAVIAIGALVTGLIWAYNNVKWFHDGVNSFFKGVSDVAV
                     EIFNFLSGYFGGFFDGVIANFNNFYNAGKRIFSGFLDFITGVFTGNWSKAWQGLVDIF
                     GGIFDGIVAVGKAPINAMIGLINGFIGGLNNIKIPKWVPGVGGKSFSISKLPYLAQGG
                     HLINGQAIVGEAGPELLTAKNGKTTVTPLSDEEKRRGIGGKVSGGNIEQHIHIGKVDA
                     NNPSELDRMNRKFAKANQQAIYDLGGVPI"
     gene            complement(95675..96139)
                     /locus_tag="IXT20_000091"
     CDS             complement(95675..96139)
                     /locus_tag="IXT20_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4858573.1"
                     /translation="MARLADYGIHVDDLKNSAVVTIQGAEFPISFTMQTMEFIADVYG
                     GDYSQFESDMNAMLSKKEGQISSANLSPSDLKIMRALIYAMLRTGGLDEDPETIFKFL
                     GMSGEVLSAYSTCMEIFASQTFQVEDLKKSKKPQDFQKTQAKRKVNKKNRKR"
     gene            complement(96139..96783)
                     /locus_tag="IXT20_000092"
     CDS             complement(96139..96783)
                     /locus_tag="IXT20_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015212246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAQ4858574.1"
                     /translation="MGKQDTFYFEGLDDILIAMMATPDSVGTAPTYSEVVRLPIATKI
                     GVKGNGTALEKWASSKMFRRVSRETKHEIALDHVGIPIAVMDEIKGMIAKSGVTFSKN
                     TAREFPYLAFGFIGNIEGGGKKAVWYPNTQLSNVIDEEYATAEDETKIDDVTANFVST
                     GLKYNNVMYASFDSNREEATMDLFEKFIAQPVYDEAQWKTLIAETTPPETGGSK"
     gene            complement(96790..97155)
                     /locus_tag="IXT20_000093"
     CDS             complement(96790..97155)
                     /locus_tag="IXT20_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HAQ4858575.1"
                     /translation="MPSNKNGFSEISDYLGNLSRVDPKKLSLESLEEAAKFYLEQLLP
                     NIPKSLLKKKHMSEQIKVVVEEDRVKVQFEETAFYWRFTENGTTKQKAQHFASGTYEQ
                     NKEKIEEIMTKKILDLWEG"
     gene            complement(97145..97483)
                     /locus_tag="IXT20_000094"
     CDS             complement(97145..97483)
                     /locus_tag="IXT20_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4858576.1"
                     /translation="MSISFDELKTALKSTKLPVFRDKARLGTMYPYIVYSNVSNSKKM
                     ASGKVYKKLPYYQISFFTLGTEQDLAVIEEALQNAGIPYSDFTGIQGDENDDTVTNYY
                     TYVRCMENAK"
     gene            complement(97473..97799)
                     /locus_tag="IXT20_000095"
     CDS             complement(97473..97799)
                     /locus_tag="IXT20_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HAQ4858577.1"
                     /translation="MPKRQTNNLRWKAELLEIKTGTDENDRPTTVYESKRLIFYEELG
                     VTSQEKYLSQQAKTDVVRRIKVRWDKSITEKLSALKIDSVTYNITRIYTNPDTREMEL
                     SLAYVD"
     gene            complement(97777..98058)
                     /locus_tag="IXT20_000096"
     CDS             complement(97777..98058)
                     /locus_tag="IXT20_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage gp6-like head-tail connector protein"
                     /protein_id="HAQ4858578.1"
                     /translation="MEEQTKELSLEEKFKSHIHFEEGMDDSLLSFYLNMAKDYVKTAT
                     GGQQEYLILMVAGIAYEYRVSEDELDKALSAITPFIVQGVIQNAETTDQ"
     gene            complement(98060..99424)
                     /locus_tag="IXT20_000097"
     CDS             complement(98060..99424)
                     /locus_tag="IXT20_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HAQ4858579.1"
                     /translation="MDTELLKKMKARREQRLTELREKVESGELREADLEAVKEEIDSV
                     IDELNGIKDELGADSGTDETDDNTDDQSNSTDSDESRSGEDNDQEEDSDSEDSSENRS
                     GMITQQQRDGLLGSIKNGLEARAKMTNKQKDQQLRKAFANFVVGNISEAEARALGIEA
                     GNGSVTVPEVIASEVITYAQEENLLRKYGTVVRTSGDVKYPILVKKADANVNKKERST
                     DIAETAIQFDEILLDPAEFDALATVTKKLLKMSGVPVEDIVVEELKKAYVRKEINYMF
                     NGDDAGNENPGALAKKAVAFEKPLDLTAAGAGQKLYDALIEFKNTPVTEVMKKGRFII
                     NRAALTAIEKMKTDDGFPLLRPFTQAEGGIGYQLVGYPVDWTDAADKKGKPDTPVLYF
                     GDFSAFKIQEVIGALEIQKLVEKFSGKNQIGFQIYNLLDGQLVYSPFEPAVYRYEITK
                     PVGG"
     gene            complement(99437..100012)
                     /locus_tag="IXT20_000098"
     CDS             complement(99437..100012)
                     /locus_tag="IXT20_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HAQ4858580.1"
                     /translation="MEMKKKEIRTIDITNLSTRSDEETHTRTISGYAAVFNSPTLLWD
                     DLSEVIAPGAFARTISNSDVRCLFNHDWSNVLGRTKSGTLRLSEDDHGLKFEVDLPDT
                     TVARDLVKSMERGDINQCSFGFVPTEETWDYNSEPMLRTISEVELYEVSIVPLPAYED
                     TEAALRSRDELEKNVEERKKLIKKINQALEA"
     gene            complement(99978..101165)
                     /locus_tag="IXT20_000099"
     CDS             complement(99978..101165)
                     /locus_tag="IXT20_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HAQ4858581.1"
                     /translation="MEKRGYIEDMFGHYTRYGQRYVTDSSIMESSDVYELVQDISNQV
                     ALSSPVVIGPDGNEVKDHHLLKILKNPNDYLTGFEFTKLETNTLLINGETFPLTDRDQ
                     LHLAYGVTTKINERLQEEFEMNGQKIPGQMIRHIKNIGTDSLKGAGIIDLARNTLEGV
                     LSAEKVLTDKYTKGGLLAFMLKLDAHINPNNSAQTKIVKAILDQLEGTQNDSDHSVKM
                     IPLGKGYSIETLKSPVDDAAILNYLGVYKKDLGKFLGINVDTYQSLMKTDIEKAMMYL
                     HNKAIKPILKNKSEHYTALFFMPNSGYRVEWKINILDFVPYSTKTNIGYNIVRTGITS
                     PDNVAEILGFPKQNTPETQAIYISNDLSRIGQKNATDDSLPTNDQNLKGGDGNEEEGN
                     SHD"
     gene            complement(101229..102956)
                     /locus_tag="IXT20_000100"
     CDS             complement(101229..102956)
                     /locus_tag="IXT20_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HAQ4858582.1"
                     /translation="MIEPGVNYADLFAKEVRKKPGKYPKTVRLAVDRWYRWKKRKDIW
                     FDVDRANEMMDWVESFIVHTKGEMVGKPFILEPWEKFIYSWMYGWVKENEKGQIVRVT
                     REAYVQIPKKNGKTLIAVGSLGYAMYGEGALSVDCYACASDFAQAQYAAKPFAATILN
                     NPILLEGTKIFKGPKGTVSSITYDYIHEDMAYSNKFIVQTKNIDNIEGSNPYFVLNDE
                     LHKQEKMEQYDNFKSAQISLPQPLMFNISTAGKGSSSVGMRVYREAKEVLKRDDNDSN
                     FVLIYEPNKNYDWTDRKVWEMCNPNWGISVDLSALESAFKTAQRSAHSKAEFLTKHLD
                     VFVNGADNFFEQDQVEPCLVTTQELGNLSGEPCYIGLDLSRTRDLTCVSLNFPTWDED
                     GKAVLKVKQLYFIPNEDLEFREKEDNVPYSDLAEQGFVEFCDGKMIDQDQILQYIEDC
                     MDLYDIQQVNYDPAMSDKLVEKLENLGLECVEVAQYPKVLNAPFDDVERLFYEKRIQF
                     DNPLFLYCTLNVVAITNINGQKAPSKRQSKKKIDGFVAFLCGHKETMNQMTDIDSDEL
                     DDYLSSIYR"
     gene            complement(102953..103405)
                     /locus_tag="IXT20_000101"
     CDS             complement(102953..103405)
                     /locus_tag="IXT20_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P27 family phage terminase small subunit"
                     /protein_id="HAQ4858583.1"
                     /translation="MTTKAQRKAIVDEKVSAEKARILKIMNLSDLYTITLDPLIESYL
                     DIFEIYQHKYLLWKEKGFPETQKFTNKSGATNQSKHPLAQQVETWSDKKMKALDLLGL
                     TNKAKTGRQITGGSTARKDEEITRPEEKPVDELAAHRNKWRKKAGTEK"
     gene            complement(103518..103898)
                     /locus_tag="IXT20_000102"
     CDS             complement(103518..103898)
                     /locus_tag="IXT20_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HAQ4858584.1"
                     /translation="MKYCQFDGCTNKIAKGIYCTEHKRSSRSRKKKQQAKSVYHHENK
                     PFYRTQAWKDMRQFIYEREGGHCQRCGQFIFGKRAHVHHIVPIKDNELLKLDPNNLML
                     LCSKCHPIVENETEDKKVFPSYFN"
     gene            complement(103895..104281)
                     /locus_tag="IXT20_000103"
     CDS             complement(103895..104281)
                     /locus_tag="IXT20_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4858585.1"
                     /translation="MAIIKIRTSITGTEYWDSEKKKTVVVPKGQEPDFEVTEEKGILS
                     DGKTFVAVNGELITNSSEILDSDGNTAADFDGDEATNDQSTEDTDELDNMTAKELRAY
                     AKKHGIDIPGAIRAKGDIINLIREAE"
     gene            complement(104262..104648)
                     /locus_tag="IXT20_000104"
     CDS             complement(104262..104648)
                     /locus_tag="IXT20_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4858586.1"
                     /translation="MNDFHEAVLSIEVEPSLATAYKKAIEDDNSRHWIKNEIKNADEN
                     IVISDIKPSWNGNHCSVSIDNSLTIEKYSGAIIDRTERKTHLTIALISRTLPNLKEQV
                     EWYERMGAKVISTNYKGENQNGNNQN"
     gene            complement(105761..106237)
                     /locus_tag="IXT20_000105"
     CDS             complement(105761..106237)
                     /locus_tag="IXT20_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1492 domain-containing protein"
                     /protein_id="HAQ4858587.1"
                     /translation="MRFQWLKDYQELDEQILYLKWNLNKSKLELNRWVNGDLADVRIE
                     KNSRSASLEENIQKIENELRLLIDQKEEMLLLIDSFSGIDNQIVKMKYVNQMSLEDIA
                     EDIGYSSSYVRQRHAEIRKTLNFLDEYENRQADRLKKENEIDFYNSKKYKEQLSLF"
     gene            106583..107010
                     /locus_tag="IXT20_000106"
                     /pseudo
     CDS             106583..107010
                     /locus_tag="IXT20_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301747.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(107117..107308)
                     /locus_tag="IXT20_000107"
     CDS             complement(107117..107308)
                     /locus_tag="IXT20_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4858588.1"
                     /translation="MMIQLAGIQTGKIYFSGESKSEASQWLLKTYTNNKKLRKKYPDA
                     LLKDDQIMPEPMIFTKWDK"
     gene            complement(107316..107504)
                     /locus_tag="IXT20_000108"
     CDS             complement(107316..107504)
                     /locus_tag="IXT20_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4858589.1"
                     /translation="MRSTDPVIILEEAKFIWTHEEIEQARLLFSQGVKPSKVAEIMDQ
                     KILDVGLLLLHLAEKNLI"
     gene            complement(107501..108076)
                     /locus_tag="IXT20_000109"
     CDS             complement(107501..108076)
                     /locus_tag="IXT20_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4858590.1"
                     /translation="MTKQVNFRPELKKVTSKSNGNTEVLLVVSNGSLRGSTENLTEFL
                     GSTVTVVIQPETIEYTVPVNKQTKKPNIEYVVNSDGTIEMLKEEQTSLDVGDGVEEVE
                     NVTILVSKETIDKFIKTATTLQLPENVTANIRDVLIRLDEGDSMSEIAADHELSETAL
                     IDQIELARQYFAPYADSWSKHKDDIIFPEEQ"
     gene            complement(108078..108533)
                     /locus_tag="IXT20_000110"
     CDS             complement(108078..108533)
                     /locus_tag="IXT20_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1064 domain-containing protein"
                     /protein_id="HAQ4858591.1"
                     /translation="MATRSKYGNKKHEVDGITFDSKAEARYYMKLKRNGMSFMPLSET
                     YCAMQENVLLQEGYLCNDRKIAPIYYRADFVIYENGQVKKVIDVKGYQDAISMLKMKM
                     FAHRYGFPVTFAKFDSKINKFIEMDCFESARQQRKRQTERRKKKLMEGK"
     gene            complement(108676..108918)
                     /locus_tag="IXT20_000111"
     CDS             complement(108676..108918)
                     /locus_tag="IXT20_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4858592.1"
                     /translation="MTFVVKKMCYLDSRGRGEASVEFAKHHTTKEEADLVASVCGGEV
                     VEVIKPERRFSKPKTKPVKKECCCPKSNQAWMRGAK"
     gene            complement(108915..109763)
                     /locus_tag="IXT20_000112"
     CDS             complement(108915..109763)
                     /locus_tag="IXT20_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAQ4858593.1"
                     /translation="MNKKLSAMAAPYGGLKTADHNCPKCGDPLYIWKTKNKDGTDRCG
                     PTCINKICGYREMVTKNQKEAIQKANEAMKKDAINRMLNSSMITDDAIWTFDFDGYKV
                     VDQETAQIKAMAQEWAKKIVSGSTIHAVITGRTGAGKTHLGAAVIKEVMMASNYKVAC
                     SFISYRELLEQLKFAMNDPEARKSVTGSLMAEIKKTDFVVIDDLGAELGRMEENNQAT
                     PYDVDVLTSLTEARLNKATIFTTNLSSKQLKHAYGERVFSRVMNGTKGNIAVFKTTTD
                     KRRSPV"
     gene            complement(109763..110542)
                     /locus_tag="IXT20_000113"
     CDS             complement(109763..110542)
                     /locus_tag="IXT20_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ4858594.1"
                     /translation="MAEGWVKLHRSITENWIWDNPQYLKWWLDLILMANHKEKKILFN
                     GSFKKVDVGQRITSEQKLAERWGVSRNTVRKFLSLLVEDDMISIEKSRKNGTTYKVNN
                     YAIYQGFSEEKKQQTEQRAEHQKDNELNINKNEKNEKNDKNNNNLRNSRKTREYADDD
                     PNKKLAILLLKLIRKNQNIKEPDLDKWANTIRLTIESDKRTGREVQDMIVWATSNDFW
                     SGVILSPTSLRKHFDKMAVQKNKRKQQNISNDELPETGEDW"
     gene            complement(110558..111373)
                     /locus_tag="IXT20_000114"
     CDS             complement(110558..111373)
                     /locus_tag="IXT20_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4858595.1"
                     /translation="MSQNLKTTSTLSDENYYSNEADWQYMSTSQYKSFLKCEAAALAK
                     LKGDWSPTSDPKALLVGNYVHSYFESKEVHEAFKEENKSKMFSSRKPYGLLKDFQIAE
                     QMIERLKQEEAFMNIYQGEKETIVTGELFGTTWKGKIDCLNVEEEYFVDIKTTKDMHE
                     RKWNANYGSRETFIVNFGYVLQMAIYQELLFQQYGKSFMPIIAAVSKQTPSEARLITI
                     DQDNMDYELVMLKEKIERIVRVKNGEEKPNHCGLCEYCRGNLPITGFTSMDDL"
     gene            complement(111336..112358)
                     /locus_tag="IXT20_000115"
     CDS             complement(111336..112358)
                     /locus_tag="IXT20_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecT"
                     /protein_id="HAQ4858596.1"
                     /translation="MANDLTQTTQRSLDEQVISNLGRLQEQGLEMPPGYSPQNALKSA
                     FFELTNNTGGNLLQMAANNQEMKTSISNALLDMVIQGLSPAKKQCYFIKYGNKVQLMR
                     SYFGTMAVLDRVTGGADITPVVVRQGDEFEVAMDGPNMVVKKHETKFENLDNEIIAAY
                     VVIKLANGKETTTVMTKKQIDQSWAKSKMKDSGPQKEFPEEMAKRTVINRAAKALINT
                     SNDNDLLVQAAKDTLENEFDNDRKDVTPQTEKVATLEQKFFSNKKITEPIQKEPDPIV
                     IPDDIQDEVTRVADVPGHSEIEQAHLIENEDTDPIQEELLDIPDFGREEGVDDVSEFE
                     DDEYPF"
     gene            complement(112361..112555)
                     /locus_tag="IXT20_000116"
     CDS             complement(112361..112555)
                     /locus_tag="IXT20_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4858597.1"
                     /translation="MKDFDSLGARQQPPNEASPVGVDWQGNSLYQGDSCYLTEEGYVP
                     VDAILEYIQQHYPKIELGGI"
     gene            complement(112565..112789)
                     /locus_tag="IXT20_000117"
     CDS             complement(112565..112789)
                     /locus_tag="IXT20_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4858598.1"
                     /translation="MISVKGLGDEIFEAMMHKAQQDVQEKILTAASYGQTSCTIRSKG
                     LTPSFLAALETEGVSSIEQDNDTLKLFWEF"
     gene            complement(112789..113118)
                     /locus_tag="IXT20_000118"
     CDS             complement(112789..113118)
                     /locus_tag="IXT20_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4858599.1"
                     /translation="MNEKIQNLIKELAAECANDDLGMSVSVVDEKGEVALGQAGNDSL
                     VAWSVYQQYEKTKRDLQNNKCNCETHGTLKQLFGIEYEDPEFEEWYQDFLRFAEKIDR
                     KKGGIKF"
     gene            complement(113150..113818)
                     /locus_tag="IXT20_000119"
     CDS             complement(113150..113818)
                     /locus_tag="IXT20_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002418651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4858600.1"
                     /translation="MSYNKWTREEEQLLIQNVRYDHRGFVCNCNELAELLGIDKKRIW
                     PKIHQMRKKDLIDEVYWDDPIEPPNKRYSQQEDKKIISMYQSGCPVTIIAQELNRTEN
                     AIRCRLNIMKKNGQLESNRKRRYTKREVELLISEIKFDKNGYVLNIDYLTRLLHRPKH
                     ELFRKICLLRKQGEITIKPDRTKASQNWYDVMKKQIDNHHKLIAARYSYQKKTRPPAS
                     KIVS"
     gene            complement(113837..114109)
                     /locus_tag="IXT20_000120"
     CDS             complement(113837..114109)
                     /locus_tag="IXT20_000120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4858601.1"
                     /translation="MTEITITDGHMSHTVKTKEEAQRIIDRYFPEGESTMQIRKPIVE
                     RSSSKMLLATLAEHKKPVYKARQLQALCYISLVLNVILLSVVFAVL"
     gene            complement(114123..114389)
                     /locus_tag="IXT20_000121"
     CDS             complement(114123..114389)
                     /locus_tag="IXT20_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4858602.1"
                     /translation="MNILSEEFLTRLRIAIVEVVKDALNQLSKKNLSETRYLKKIEAR
                     KYVGGVNDQGFEKLVDHGLKEIRIDGFLRYDKKDIDELMAKYKI"
     gene            complement(114410..115165)
                     /locus_tag="IXT20_000122"
     CDS             complement(114410..115165)
                     /locus_tag="IXT20_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor Ant"
                     /protein_id="HAQ4858603.1"
                     /translation="MKELIKVTTNEENEQLVNGRELHEFLEVATEYKKWFSRMAEYGF
                     VENIDFVRVTQKCPTPGGIQNITDHAMKLDMAKEISMIQRTEKGKQARQYFIEVEKEY
                     KQQLLDTSKLSPELQMFQGIFNAVAKQELETKRLATQMENITEIVALNTTDWRRECRK
                     LVNKMAETQGGYGAYQEIQTAIYEEVDRRAGSSLKTRLTNLRNRMAGEGVPKSKRDKT
                     NKLDVIESDKRLKEIYLSVVKDFAIKYGVWKEK"
     gene            complement(115208..115456)
                     /locus_tag="IXT20_000123"
     CDS             complement(115208..115456)
                     /locus_tag="IXT20_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ4858604.1"
                     /translation="MELQITLEAARVMMGYSLKEAAKLFDVHHQTLANWEQDPNKMKQ
                     KYVQLIPEIYHFPTANIFFGSKDEFIRYKLHNDSFLIK"
     gene            115621..116460
                     /locus_tag="IXT20_000124"
     CDS             115621..116460
                     /locus_tag="IXT20_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ4858605.1"
                     /translation="MPRKPLSEEDKKARSIIANNLKKITHGYTRAQLSDMTGIPVTTL
                     SGYFTEKSTISMENAELIAKAFNVDKSDIDPRYSREYNYYDLKKEVLDELQENNKSFV
                     ADKFVELAEKANAAGMTLNRTKTDRGNIMTVLSSDIQEYNFFDTSVAAGIPSSVEAFD
                     QDHIEQIAIPDYAMGKYAGYQDVFFTKINGDSMNNVIPDGSLIAVKKIESCDELKDGD
                     IVVFSNDNEFSVKRYINDQQNNRIIFRPDSSNISFTDIVVNYDDTKYVQIYGKVVVYI
                     VQL"
     gene            116481..116681
                     /locus_tag="IXT20_000125"
     CDS             116481..116681
                     /locus_tag="IXT20_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4858606.1"
                     /translation="MKIGMRKPSIKKSIGARTTGKAKRAVKKAVIPGYGKKGSGWIKD
                     PKKAAYNKVYKKTTFSFWDLFK"
     gene            116767..117207
                     /locus_tag="IXT20_000126"
     CDS             116767..117207
                     /locus_tag="IXT20_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4858607.1"
                     /translation="MKFIKFGIIGLLVVTVMGGCGTSTAETKESKNYSETNSTIKEVD
                     SDQTAEFATIAEKHIKEIYSIDNLKMDLKSIKVTQFPDDKNAETGEEYKNVYNGTGDF
                     TWQDKKYNFSLIYSKKDATNYTVLYLYSNLDPNKGVDTPLESDQ"
     gene            117346..118551
                     /locus_tag="IXT20_000127"
     CDS             117346..118551
                     /locus_tag="IXT20_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAQ4858608.1"
                     /translation="MAKKEIDKRIKKHLTKKGEEKYQFSLYLGVDPLTGKKRKTTRRG
                     FSTPLAAERALKRLEADIQEKGLQASTAQKSKKFEYVYGLWFENYKKTVKESTWSSTK
                     QIFDTHILKVFGDKFIDKIDVFFCQEAVNTWSDSHPKIFKKIKNYTSNVFDYAASLQI
                     ITSNPMKLVSVPRGEALDIEDKNIEFYTKEELIEFLEAIRNDDDERYLFFSLLAFTGI
                     RKGEAFALTWSDIDFKSKTLNINKTVTRGYQGRLIVNTPKSKSGKRKIYLDNDLISLL
                     RKYYTKNKTIVTIQSENLIFQHDGLLYNPTVSRSWLNVIYKHHPELTKRITTHGFRHT
                     HASLLFESGASLKDVQERLGHADIQTTSNIYTHVTETQNKKVINNFVAFMKNSTQGES
                     KGESKNILG"
     gene            complement(118603..118690)
                     /locus_tag="IXT20_000128"
     tRNA            complement(118603..118690)
                     /locus_tag="IXT20_000128"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(118656..118658),aa:Ser,seq:cga)
     gene            complement(118835..119401)
                     /locus_tag="IXT20_000129"
     CDS             complement(118835..119401)
                     /locus_tag="IXT20_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAQ4858609.1"
                     /translation="MRRKVYTKDQILKAAYDVVAKEGFKGFTARNIAKKMGISTQPIY
                     LEFKNMEDLKNTLLDSIFEQLKEDVFPIERTGDKIIDLGLNYIYFAKKHHSLYMALYI
                     DEHGDGKKMHDFSYNYFREIVKEDERYNQLSDEYVDALHTGTWVAVTGLASLMSSNIM
                     HPTEEQIIALIKRTIDSILALDNPEIIR"
     gene            119657..120988
                     /locus_tag="IXT20_000130"
     CDS             119657..120988
                     /locus_tag="IXT20_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1576 domain-containing protein"
                     /protein_id="HAQ4858610.1"
                     /translation="MENETDVIYIHPQKRIVSQKRKYFYLGFTGVFFLFIGLLSNTPT
                     DNWSGLLTILTSPSNLLTDYFALGGFGSAFINVGILTLLSVLLAYRHKVILNGPLFAS
                     ILTVTGFSFFGKNLYNSISIILGVYLYAVFVNKPFSQYIMIGLFGSALSPVVSYITFG
                     MRFPLLIGILLGNLAGIAIGLLLPPLAAQTLVFHRGFTLYNIGFTSGLIAMTFTAVLR
                     LFSYSIVENTLVFNEYHFPLIWIIFGFFSLTVGIGFYYNSFRLLGIREIFDSSGKLTT
                     DFIANSGIGATLINIGLVGLMLSSYVLLVGGQLNGPVIGAILSAVGFSAFGCHLKNSF
                     PILVGIFIASLFGTFHEITSTGMLVAAVFGTGLAPISGFYGSFYGVIAGVLHIALVHN
                     VSTLHGGLNLYNSGFSTGFVAGILVPILDNFTAVRKEKKTLGKRIIKKNHR"
     gene            120954..121304
                     /locus_tag="IXT20_000131"
     CDS             120954..121304
                     /locus_tag="IXT20_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4858611.1"
                     /translation="MEKELSKRIIDELLTYLYSHEITEIRIGVEFAEEGLFIEIQGTA
                     ETQPEDLDVLADLLNNPRDPSMEIYYQELLGNPRHDREDYHLLGSLIDDVEIMYAQPI
                     INIKIFRRASHSYY"
     gene            complement(121396..121815)
                     /locus_tag="IXT20_000132"
     CDS             complement(121396..121815)
                     /locus_tag="IXT20_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HAQ4858612.1"
                     /translation="MTTKHEEIHNGVIFPAGEKNEAYAQVFTGQSYLNMLVNDPEVSV
                     GVGNVTFEPGCRNHWHVHKDGYQILLVTGGEGWYQEEGKPARRLMAGDVVVTHKGIKH
                     WHGATKDSWFEHLAITAGNSEFLEPVTDEVYEGLENQ"
     gene            complement(121816..122160)
                     /locus_tag="IXT20_000133"
     CDS             complement(121816..122160)
                     /locus_tag="IXT20_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HAQ4858613.1"
                     /translation="MAEKQTAGRDQLGDFAPKFAELNDDVLFGQVWSREELSAHQRSI
                     ITISALISGGNFEQLPAHLKIGKQNGITSEEIAEIITHLAFYVGWPKAWSAFSLAKEI
                     YLEENSEDKKEA"
BASE COUNT        34656 a        25825 c        20643 g        41206 t
ORIGIN      
        1 tataaacatc accccaaata aaacagaaaa cagtataagc attataaaaa ttatcaacaa
       61 ccttgatcct tcttttatag attcaggaaa cagtataatt ttagtactag ataaattgtt
      121 tacaccgatt agtgttaaaa tatctttatt cttcttgcta acataaccct ttatgcacaa
      181 aagaacagct atacttaaag taacaaataa ataacgtatt ccaatagaaa aatcactata
      241 aatactctct agcataggac cgacaattga agaagtcact actgcaaatg ctggacctat
      301 ccaagcaaca ttagaatgtt ctttctctct tttcctttgt aaatctttgt tgagtattaa
      361 tctatttatt tcttctgcag gcaagttaat tttcataacc ttatggggga ttatccaatt
      421 aaaaatcgga aaaaggtagc ctccccaaaa agtatagtga tctgtgtcaa tcagataata
      481 acctgatttt gattccagaa taacatatct caaagccgaa cgaaaatttt tcatgaatat
      541 ccataatctc actgtttcca cacctaattc ctccaattcc aattaaatgg atttatagca
      601 tcaccaatta tgttaactgc ctgaccaacc tcattgataa aattggcacc actatcccat
      661 gcttcaacaa tgctatatat ctatcaaaca aaaaatggaa aactaaatac tcgcgcacat
      721 aaattttaca ttctgttttt tataagacta ataataatca ttgaaaaaat ttcttatcgg
      781 tggcgcatat tcccaattga ttaatgtgat cccatattct gcaagtattt tacgagcttc
      841 ttctaagtca tagtgagaaa aatgcataaa acctgcaata cgatcacgga ctgtgagctg
      901 tggcttgctt ccaccatcat tcataaaata tccagcagag ccactactta aagtgattgt
      961 tacatcttca ctttctatta ttaagaattc ataatgaaat gctttcaact ctccactatt
     1021 atttaaaagt tttatactcc aacttcgtct aggttgagtt gtcacattcc ctctaaaact
     1081 tcctataaat tctgcaatca ccgtatcttc aatcaaaatt ctttcctcat ctatttcata
     1141 ttcaagacta aacggaaatt ctccatatgt aatctctggc aatggtggtg ctgtagttct
     1201 ccaaataaat acaataactg tgaatagaaa aaatccaacg atcgatataa atgttatgag
     1261 acctgctcga agtaaaaccc gatataataa aaatgctgta tttcgctttg ctatatctag
     1321 aaaaataaaa agtactgcaa tcataatgat agtaaaagtt gtaaaaaagc acattttttg
     1381 ccctcccttt ataaacatcg attctcaaat tctaaaccaa atcttgatag ttcaagttca
     1441 ggtataacgt ataccaacca tacatcccaa ttactattta tgaacttatg attatcactg
     1501 aataaatcac tccatcccct tgttaaaact tctacattaa ttacacctgg ttgttttcga
     1561 ctagctaatc caatgaactt atcaaccatc cttatccacc aaccacgaga atcttgacca
     1621 gaagtaagaa aaacagttct caaggttgat cgagcagttc cttccagttt ttaattaatc
     1681 tccagttata gataaatact tgatcctatc tatatcagga cttctatatt aagcaaaagt
     1741 ttatgtataa aactaaaatc ttcattcagt tgtctccccg ctttttatac gattgtatta
     1801 aaaaacaata gaagcctttc aacattatgt tactccagta aagttggaat tatagtttga
     1861 cggaagaaaa gcaagtatag cttttaaaaa aaactgatac attcgcatct cctgcttcat
     1921 gtatcagttt tttctttgga ttaaactgct tcttcacgca gtttttccgc aaattcattt
     1981 attttgcgaa ttcggtgatt aattcccgat tttgatattg cacctgaggg aatcatttcc
     2041 cctaattctt tcaagctgac ctctggatgt tgaagccgaa gttctgcgat ttcctgaagt
     2101 ttttcaggaa gggcatgtag accgaccttt gcttcgatca attcgatgtt ttcgatttgt
     2161 ttagaagccg catcaatcgt cttgttcaga tttgctgtct cacaattgac tagacgattg
     2221 accgaatttc tcatatcccg tacgattcga acatcttcaa atcggagcat tgagttggtt
     2281 gcaccaatca atgttaagaa atcagcaatt ttttctgctc cttttaaata cgaaatatag
     2341 ccattgcgcc gttcaagcgt tcgagcatgt agaccgtaat aatttaacat atcgcaaatg
     2401 tcttggttat gctgttcata aatagaaaat atttccaaat gataacgact cgtttccgga
     2461 ttattgactg aacctgaggc cataaaagca ccacgtaagt aagaacgcat tttttgcgaa
     2521 tttcccataa tctcgtccga aacgtgtcca ttgaacatca taccatccat gatatcaaga
     2581 tccatcaaca catcttttgt gccttgcttc aaccggacga tatagacatt gttctttttt
     2641 aatttcattt ttttacgaac gagtagctca cttcgtacct gataatgatc tttcaaaaga
     2701 gaatagatac gtctggcaat tgcagcattt tctgtttgga cgttcaaaat aaactgctgg
     2761 ttcacaatac ctaaagagcc gttcattcgc agcaaagcag ctaattccgc tttcgcatgt
     2821 tctcgatgaa cttccaaacc agttagttct ttcttcacat ctgcagcaaa tgacatccta
     2881 ttctcctcct ttctccaaca ataaattgct taatattttg aaccgaaaac taaacggaac
     2941 aattcttcta ctactttgtc tccatcgtgg aagacaccac catctttcaa ttctaaaaag
     3001 tcggtggaaa tcactcgaca tccttcattt ctcaatccgt tgaagtcgtg tgcgacttgt
     3061 actagatact catcatagat ctctgggtcc atataatctt caggcacttt ttccgtattg
     3121 accaatactg tatcgataaa aggctgattc aaatgcttgt gtaaaacacg tatatgatcg
     3181 gcatctgtga aatgttccgt ttcccctttt tgggtcatga tattacagat atagacggtt
     3241 tcagcagttg tcttgagcat tgcttcgccg atttctggta tcactaaatt tggaaggata
     3301 ctggtgaata aacttccggg tcccaaaacg accatgtcgg cgtccataat tgctgaaacg
     3361 acttttcgag ccgctttggc ttcactttta tcatttgtgt tgcagacata cacacggtca
     3421 atggttttac ggttgatcgc gattttagat tctcctactg cagtcgttcc gtctttaaaa
     3481 acagcatgca aggtcaaagg agtttctgaa gaaggataaa catgtccatc tacatgcatc
     3541 atttttgcta ataattgaat cgcctcataa gtgcttcccc gcatctcgga aactgcggca
     3601 atgatcagat taccgatcgt atgattggct aaaaactgat cttccttttt gaaacggtat
     3661 tggaaaatat cttcatagaa ttgtggcata tccgataacg ccactaagac atttctcaag
     3721 tcaccaggag gagccatagc gatcgattct ctgattgcac cgctgcttcc accatcatct
     3781 gcaacagtca ctactgctgt gatatctgct ccttgatttc gtaaactttt caggataact
     3841 ggcagaccag tgccaccgcc gaccactacg atctttggtt ttcttatccg gtaggttttc
     3901 attttcacga ccggttcacc gtctctttcc ttttatcctt gtcccgatgt gtcacattga
     3961 ctttatagtt tttccctaaa gcatttgcca aacgttcagt caatgccaca gaacggtgtt
     4021 gaccgcctgt acagccgatt gcaactgtca agctgctttt tccttcttta atgtatccag
     4081 gcaatacagt ttccagaaga tccaaaaatt tatggtagaa tgattgcgtt tcatcaaagg
     4141 acatcacata atcatagact ggctgatctt ttccggtcaa tggtcttagt tccgggatat
     4201 aatgagggtt gggcaaaaaa cgcacatcca tcacaatatc cgaatcgatc ggcaacccgt
     4261 atttaaaacc aaatgataca aattcgatcc gaaatccttg gtctcccttt gatttgaatg
     4321 actcattgat tttttcacgt aattgtcgtg gtgtcaatgt tgtcgtatca atgatcacgg
     4381 aagcttgggc tttcaagtcg tctagtatcg cccgttcttt acgtatccct tcagtgatca
     4441 aaccgtccat cgccattgga tgtgctcgtc ttgtttcttt gtagcgagcc actagttctt
     4501 cgtctgaagc atctaaaaat aagatagaag tgtcgatgaa gttcgtattt tcgatttcga
     4561 tcagcatgtt ttggatttct tcaaagaagg aacgagaacg caaatccact cctaaagcca
     4621 tttttgtgac tttacctgat tctttgatca attcccagaa ttttggaatc aatcgaggcg
     4681 gcatattatc gatacaaaaa tagcccatat cttcaaaact ttggatagct actgttttcc
     4741 ctgcaccact catacctgtt atgatgacta attgcaaatt atctgtcata tttacgaacc
     4801 cctctcacat tgtgcataat aaaattatat catcccgggc gtatcattac cagttttctt
     4861 tttcttttac aaaaagattt ggaccaaata tcttttagga tatttgcccc aaatccaatt
     4921 tttttacttt tccgatgctg tatccaatgc ctcgcttctt tcagctaatg cacgttcgat
     4981 cgttcgatcc gctaaaatag attgagcatc gatcactggg taatgattat cttcagcaaa
     5041 ttcatgcatc aaagataatt ctgtgcaacc taggatgact ttttcacagt ttaggcgctc
     5101 taccgcttcc tctaagattt cgtaatagag ctcttgattc aaataatccg attccttgat
     5161 ctcatgatag atcaggtaat tgattttttc ttgaagcgca gtatcaggaa tcactgtttc
     5221 gaaaccaaga tttttgactt cgcgttcata gattccagct ttcatacttc cttctgttcc
     5281 tagaatcgct actctccctg tcgtatgctg acggacaagt tcatttgctg cttctcttgg
     5341 catatgcaag ataggaatat ccgttgccgc ttgtaactct tcaaaaaagt agtgtgcggt
     5401 gttacaagtc aacacaatga agtttggtcg caataaattt tgtttctcaa tatcatccag
     5461 cagaaacggc attggatttt cttctgatcg atctaaaata taagctgtac gatcaggaac
     5521 tgttgcatga ttgaataaga cataattcaa atattcttga tcttttgtag ctttcgttcg
     5581 gtggttgatc aagcgaacaa aactttctgt cgccatcgtg cccattccgc ctaaaatact
     5641 gaaaaaattc tccatgaact agccttcttt cacatgaaaa tactttttga atcttggaat
     5701 atagttatgg aacatatatt tcatcaacaa ccagcgcttg attgatcggt ctttttcata
     5761 aaatacagta gtaccgtaac gattttcttt gatcatttgc tcagctagtt ttttgtcttc
     5821 attttcccga gcatattcta aaaagatttt tttgggcaca cccatccata atcttgcttt
     5881 atctgattga ttcgttccat aggtcgtttc aacaaagggt ttgttgaaca ctcgatcttc
     5941 tgtgacaaaa cgagccagat tcaagccgtt caatgtcaca aagaaactgc tacgaccttg
     6001 tctcaaattg atttcaaata atttatattc tccatctcta ggatcatact tcatatcgaa
     6061 attggcaaag cctgtatatt cgattttttc caaaaatgcc ttaatggttt gatagatttt
     6121 ttcgttataa tctggcatga tcacaacgta attaccaatc gatgctggtg taggatcttc
     6181 taatagtgga tgaccaaggc acatcatccg aacttgatga tcttcatcta catacgcatt
     6241 tagcacacgc atattactat catcacccgg gataaaatct tgtacaatca tttcactttt
     6301 ataaccggct tcgtatatcc ggcctaaaat taaatcgaac tcttctctag tctctaaaat
     6361 aaaagctttt tttcttcctt caaattggac agaaagatac tccacactgt ttgcaggttt
     6421 caaagcaact gggaattcaa aaggcaattc ctgttccaaa tgtccattta ctaacatttc
     6481 ttctctgacg atcaatgttt taggatacgg aagatcatat tcttcacaaa cttcataaaa
     6541 gcttacttta ttgatcagcc tttcaaataa agaataatca atatatggac agataaaata
     6601 ttctgacagc tcttgtttat gttgggagat caattctgca tatccatcac cacaagcaat
     6661 caaaagatat tttttactct tatcagaata tctttctttt gccaaacgaa gcatcgtctc
     6721 gataaataca gggtctttat caaatcctgg aataacttct acattgacaa ttttactgta
     6781 gcgggtaggg gctaactgat ccgaagcata tgcttggcag attttgccgt atgcttcatt
     6841 aaaagaacgt gccataccat aaacgttcat gtcgcttcct aataatatag ggataaattc
     6901 ttcattttca atactgttca tcattatctc tcacttactc ataatctctt gtttttcttt
     6961 tccaactaca caaggaatat cataccataa agaaaaaagc aatcaaagga aagtcctccg
     7021 attgctcaat tcttttttga acggattgaa cttataggaa gtttttatct ataccaatca
     7081 gtgtagaaag aactaccatg taatgattct agaacactga tattaataaa acaagcaaaa
     7141 attcattttt ttatgttttt ttaactatct cagctatcta tcctctttat accattattt
     7201 ttttcaaata gtgacctgta taactttctt ttacgttaat gatttcttct ggtgttccag
     7261 tcgcaaggat cgttcctcca ccttctccac cttctggtcc taaatcaatg atataatctg
     7321 ctgttttgat tacatctaga ttgtgttcaa tcactaaaac tgtgttacca gcatctacta
     7381 atctttgtaa tacatgcaac aagcgggcga tgtcatcgct atgaagtcct gtcgttggtt
     7441 catctagtat atagaaattc tttccattag agattttgtg caattcactg gcaagtttca
     7501 tccgttgtgc ctcaccaccg gacaatgtcg ttgctggttg ccccatcgtc acataaccta
     7561 agccaacatc aacaatcgtt tgcagtttgc gatgaatctt tggaatgtgc ttgaagaatt
     7621 ctacagcatc ctctactgtc atttccaaaa tatcagcaat gctttttcct ttgtaatgca
     7681 cttctaatgt ttcagaatta tatcgtttac catgacatac ttcacaagga acatagacat
     7741 caggcaaaaa gtgcatttcg atcttaataa ttccatcccc gcgacaagct tcacaacgac
     7801 cgccttttac gttgaaacta aaacgccctt tcttataacc ccgcattttt gcctcgttcg
     7861 tttgagcaaa taacccacgg atatcatcaa atacacttgt ataagtcgct ggattacttc
     7921 tcggcgtccg gccgattggg ctttgatcga tatcgatgat cttttcgata ctttcgtagc
     7981 cggaaattgt cttgaattta cctggcttag cagaattctt atttaatttt tgcgctaacg
     8041 atttctttaa gatactattg accaatgtac tctttccaga accagacaca cctgtgactg
     8101 ctacaaattc tcctaatgga aattcaacac taatattttt cagattgttt tctgaagcac
     8161 cagtgatctt gattgctttc ccattacctt tacggcgttc ttttggtaca gggattgatt
     8221 ttttccctga aagatatttc cctgtcaaag aattttcatc attagctact tcttcaggag
     8281 tgcctgcagc cacgatttgt ccgccttgtt ctccggcccc aggtccgacg tcaatcaaat
     8341 aatctgctgc ccacatcgta tcttcatcat gttcgactac aacgagggtg ttgccaagat
     8401 cacgcatttt ctttaatgag ccaatcagtc gatcgttgtc gcgttgatgc aatccgatcg
     8461 atggttcatc taaaatatac aaaactccag aaagattcga tccaatttgt gtagccaatc
     8521 tgattcgctg cgcttctccc ccagataatg ttccggaagc gcgactcaaa gttagatagt
     8581 ctagtcccac attttccaag aatgaaagtc tgtcatttac ttcttttaaa atcggtttgg
     8641 caattacttt ctcttgttca gataacgtta agtttgagaa gaaatctact gctttttgaa
     8701 tagacaactc atttgtttgc ccgatattcg ttccattgat ttgaacagat agagcttggg
     8761 gattcaatcg ataaccttta catgtctgac aagttaattc tgtcatataa agacgcattt
     8821 gctcacgcgt gaaatcactg ttcgtatcat gataacgacg ctcgatattc ttcaataccc
     8881 cttcgaaagt cgtttctaca tcgcgaacgc caccaaaatc attttcataa tggaaatgga
     8941 aaagatcatc tgtcccattc aagatgatct cctgatgctc ttccggtaat tcttcgaatg
     9001 gtgtatctaa atcgataccg aaactttcac aagcctgcgc taacatttga ggataatact
     9061 gagagctgat tggattccat ggaacgatag caccttgact aagtgtcttc gttctatctg
     9121 gaatcactaa atcttgatcg acttctaatt tgacacctaa accatcacag tctggacaag
     9181 ctccaaaagg cgcattgaat gagaacagtc ttggttctag ttcgccaaca gtaaatccgc
     9241 aataaggaca ggcatagtgt tcgctaaaca acatttcttc ttcaccgatc acgtcaacca
     9301 gagcatagcc gtctgctaat cgcagggccg cctctaaaga atcaaatagg cgagaacgaa
     9361 tcccttcttt cacaatgatc cggtcgatga cgatcgcgat atcatgtttt ttgtttttct
     9421 ccagctcagg cacttcactt aaatcataga tctcaccatc gactcgaacg cggacgtatc
     9481 cctctttttg gattcgttcg aatatttttt tatgttgtcc ttttttcttt accacgatcg
     9541 gtgccaacac ttggatcttt gtgcgctcag gcaaagccat tacttgatct gccatttgat
     9601 ctactgattg actggtaatc tcgatatgat cgttgggaca aatcggatgt cctacacgtg
     9661 caaacaacaa gcgtaaatag tcattgattt ccgtcacagt acctactgtc gaccgaggat
     9721 ttttacttgt tgttttctga tcaatagaaa tcgccggact caatccgtcg atgctatcta
     9781 cgtccggctt atccatttgt cctaaaaact gacgagcata agcagaaagg ctctctacgt
     9841 atctccgctg cccttcagcg tacagcgtat caaatgctag ggaacttttt ccagaaccgg
     9901 acaaacctgt aacgaccacg aatttatctc gtggaatcgt tacatctata tttttcaagt
     9961 tatgggcacg tgcaccatga atcgtaattt tatcgttagc catgttgtcc tcctactttg
    10021 aagcttttaa ttctaaaatc atatctcgca cattcgcggc tgtttcaaaa tccaaagctt
    10081 ttgccgcatc tttcatttct ttttctaatt tcatcaataa cgtgtctttt tcttccttcg
    10141 tcatttcttc gtacgagact tgtaccgctt cttttgtatc atcttccgat tctttcgtaa
    10201 tagaaatcaa atctcggatt tctttaatga tcgtttttgg aacgatccca tgttcttcat
    10261 tgtacttctg ctgaatcgta cgacgtcttg acgtttcatc catcgctaga cgcatagaat
    10321 ctgttacttt gtctgcgtac atgattactt taccttcttc attccgagca gctcgtccaa
    10381 tcgtttggac taaggaacgt tcactacgta aaaagccttc tttatccgca tccaagatag
    10441 caacaaggga aacttcaggt acgtctaatc cttcacgaag caaattgatt ccaactaaca
    10501 catcaaattc acctaatcga agatctcgaa tgatttctgt tcgttccagc gttttgatat
    10561 cactatgcaa atatttgact ttgatcccta attctttgaa ataatctgtg aggtcttctg
    10621 ccattttttt cgtcaatgtc gttacaaaca cccgctggtc tttttcgact cgttcattta
    10681 tttcgcctac tagatcatcg atttgtccca tgattggacg aacttcgatc actgggtcaa
    10741 gcagccctgt cggacggatg atttgttgga taacggtatc tgtttgttca tgttcatatg
    10801 gtcctggcgt cgcagagaca taaatgatct gattgacatg tttttcaaac tcttctaacc
    10861 gaagtggccg attgtctaaa gcagatggca aacggaaacc gtaatctact agcatctgtt
    10921 ttctcgctcg gtcaccatta tacattcctc tgatttgagg catggttacg tgcgattcgt
    10981 ccgccacgat caaaaaatct tcaggaaaga aatccaatag cgtataaggc ggttcacctt
    11041 ctttacgtcc gtccatatgc cgagaataat tttcaattcc cgacgtgtag cccatttcta
    11101 acatcatttc aatatcgtaa ttggttcgct gttctaaacg ttgagcttcc agaagtttgt
    11161 tttcatttcg caagacagtt aaacgctgtt ctaattcctc tttgatattc gctacggcat
    11221 gctccatatg ttcatcattc gtcacaaagt gagtcgccgg gaagattgct acgtgttccg
    11281 tttctcctaa aacttcacct gtcagtgcat tgacttctcg gatacgttcg acctcatctc
    11341 caaaaaactc cacacgtaag gcacgttcat ctcttgaagc cggaaaaatc tccacgacat
    11401 caccgcgcac tcgaaaacga ccgcgctgaa aatctatatc atttctttcg aattggatac
    11461 tgactaagtc acgaatcaac tgattgcgat ctagttctgc tccctgtcga atcgaaacta
    11521 cttgtttttg gtactcaaaa ggcgagccta atccaaaaat acatgaaacg gatgcaatga
    11581 cgatcacatc atttctttct aataatgaac tagtagccga atgtcgtaat ttgtcgatct
    11641 catcatttac acttgaatct ttttcgatat acgtatcgct tgaaggcaca taagcttcag
    11701 gctgataata atcataataa gaaacaaaat attcaacagc attgttaggg aaaaattcct
    11761 tgaattcccc gtacagctga ccagccaatg ttttgttatg ggcaataatc agtgttggtt
    11821 tattcacttt ctcaataaga ttggaaatcg tatatgtttt ccctgttcca gtcgcaccta
    11881 ataagatttg tgcttttttc ccgccgacta cgccatccac taattggtta atcgcttcag
    11941 gctgatcacc agccggttgg tatttcgaga caagctcaaa gtgtcgggag gtatctctct
    12001 caatcattct tcgtcctcct tttcattcat tccttttcat tttacgacca ataaaaagaa
    12061 aaggaaagca aaggcattcc ttactaaaaa attataccac accgaacata ctttcgccac
    12121 ttttttatct tattatacat gttatgtttt ataacataaa atgttagaaa ctcgcatttt
    12181 tttcattttt tcgagcttac agtcttgtct gattcagcac ttttcgccta taataataaa
    12241 atattggaaa tttaggaggt tgattatgaa aagaaagcat ttatttcact catttgtttt
    12301 cttaatgaca ggacttgtga cacttgcgtt tggtcatgtt gcacaagctg acaacactac
    12361 aacggcagag tcaagcacaa gcgagacaat gcaaaaaata gagcccaaaa aagacgtata
    12421 cgtaattgcc agtgactctg cttttgctcc atttgaattt caaaacgcaa agggcgagta
    12481 cgaaggcatc gatgtaaaat tgatggaaaa catcgccaaa ctgcaaggat ttaacatccg
    12541 aatggactac cgtggtttct ctgctgcttt gcaagctttg gaatcaggac aagcagacgg
    12601 aatgattgcc ggaatgactg taacagatga acgaaagaaa agctatgatt tttctgattc
    12661 ttactttgat agcgggatcc aattagctgt caaaaaagga gatacttcga tcaagtctta
    12721 tgaagatttg aaaggaaaaa cagtcggagc aaaagttggt accgaaagtg ctgaattttt
    12781 aagcgaccat gaatctgaat atggctattc tatcaaatat tttgatgctg ctgatcaatt
    12841 gtacgacggg ttaaaagttg gcgcaatcga tgcaatgatg gatgattatc cggtaatcgg
    12901 ttatgcgatc aaacaaggac aagaattgga aacaccgatc aaaaaagaat ctggcggaaa
    12961 atatgcattt gccgttaaaa aaggacaaaa tcctgagctt cttaatatgt tcaatgaagg
    13021 attgaaagaa ttaaaaagaa caggcgaata cgataagatt attaaagaat atgtagcgga
    13081 tggttcagaa acttccagtg agtcttccac tgctgatgaa tcaacatttc ttggacttat
    13141 ccaaaacaac tatcaagcat tgttgaaagg actttggaga acaattctcc taactttgat
    13201 ctcctttgct ttggctatag taatcggtgt gatttttgga ttattcagtg tagcaccaat
    13261 acgcgctctt cgcacaattg ctagtattta tgtcgatatc atccgaggaa ttccaatgat
    13321 ggttcttgcg ttctttattt tctttgggtt gcctggtatc gttggattta cgattcctga
    13381 cttccttgct ggtgttatca ccttgactct taacgcaagt gcttatatcg cagagattgt
    13441 tcgaggcggg atcaacgccg ttcctgtcgg acaaatggaa gcttccagaa gtttaggtct
    13501 ttcttataat cggacaatgc aaaaaatcat tttgccgcaa gcgatacgca tcatgatccc
    13561 atcattcgtc aatcagttcg ttatctcatt aaaagacaca acgatcattt ccgcaatcgg
    13621 tgtagtcgaa ttgctgcaaa ctggtaagat cattgttgca cgtacaacac aaagtacgta
    13681 tgtttacttg atcattgcga tcatgtactt gattttgatc acagcattga caaaattagc
    13741 aaaagtttta gaaaagaagg tgaagtaaga tggcagaaaa aatcttagtt gagcatttag
    13801 tcaaaaaata cggcgataac acagtattaa acgacatcaa tgtatccatc aacgaaggag
    13861 atgtcgtctg tatcattggc ccttcaggtt caggaaaaag tacattcttg cgttgcttga
    13921 atcgtttaga agaaccttct gacggtgaaa ttattattga cggcgcccac ttgatggata
    13981 aaagcacaga tatcaacttg gtgagacaac atatcggaat ggtattccaa cactttaacc
    14041 tattccctca tctttctatt ttggaaaaca tcattttagc accgatggac ctgaagaaag
    14101 aatctagaga agaagcagag aaaaaagcaa tcaaattact gcagacagtt ggtctagaag
    14161 ataaaaaaga tatgtatcct gaaatgcttt ctggtggaca aaaacagcgt gtggctattg
    14221 ctcgtgctct agcgatgaac cctgacatca tgctttttga tgagcctact tctgcactcg
    14281 atcctgaaat ggtcggtgat gtcttgaacg tcatgaaaaa gttagcagaa caaggtatga
    14341 cgatggttat cgttacgcac gaaatggggt tcgctaaaga agtggccaat cgcgtcatgt
    14401 tcattgacga cggaaacttt ttagaagatg gaacgcctca acaaatcttt gaaaatccgc
    14461 aaaatgaacg gacaaaagat ttcttgaata aagtattaaa tatttaataa aaaccaaaaa
    14521 tgcacggtcg ccatgaaggg accgtgtatt tttatcttct tattttctca cctaatcgta
    14581 tcagccgcat cgcttgtttt cctgcacaaa caagcaatcg ctctacctgt tccatcgcat
    14641 aagaaagtac catcggttca ttgatgatat caagtacata gtcgatacca ccctcactta
    14701 attgagcttg atttttcttc tccatattat gtagttgtgg gacaactttc tgacaatcag
    14761 caattgtgtc aagaacggat caatccttta ttggagttgc tcgaaagggt cttctcttat
    14821 tacggacagg ctttgctcac tgtacataga caacaaatca ttatcctagt caatcggatt
    14881 tccagagcct cattgctatc attgcttgat aaaatccaga caaagtttaa taaaatgtat
    14941 cagttacagc taaactttgg aattgggtcg ctttgttata cagagcaaga gacaccgcaa
    15001 tctttccttc atgccaaaca agtttgcgaa tggatagctt tccatcaatc tgtgaacgag
    15061 atccgctttt ttgaagattt agacctcggg atcgttttac cagcaatccc gtcagatcaa
    15121 cgaacattat atgtaaaacg tattttgaaa agtctgacag aagaagtaca tttgttcaag
    15181 aagactttgg catgcttcag taaaaataac ggaagcatca aaaattgctc agaagaatta
    15241 tttattcata aaaacacctt gcaatatcga ttgaacaagt tccattctct cactggttat
    15301 acccctagaa actatgacga ttatcatatt ctaaagctgg cttttttgtt ggtacaaaca
    15361 tgacaactac aaaaaaacca ggcgaacata tgattgttcg tctggttttt aattattaat
    15421 gacttagcgt ttttactaga aaaactactt ccgctttttc ccgccaaaag catctttaaa
    15481 cttatcgaag aaaccgtctt cttgctgttc ttcaactttg atgccgccag cttcagcgaa
    15541 tgcacgtaat gcttctcttt gttgttcatt tagatttttc ggtgtgatca gttttacttt
    15601 gacatgttgg tcgcctgttg caccaccacg taacttcgga gcgccttttc cacgtaaacg
    15661 gaagttcgta cctgtctgtg ttccagcagg gatcttcaat ttcacatctc catggacagt
    15721 tggtacttgg acttcatccc ccaaagctgc ttgtacaaaa cttaaaggca attcatagta
    15781 aatttcagaa ccttcacgat caaagatatc actttcttct acacggaaga ccacatacaa
    15841 atcaccaaat ggtccgccgt tttctccagc ttctccttga ccagctaaac gcatttgttg
    15901 tccttcttct acaccagctg gtacattcac tttaactgaa tgtgctttct tctcatgtcc
    15961 tgagccatga caggttggac aaggttcttt gatttctttc cctgtcccat gacaaacatc
    16021 acaagtttga cggctcatca ctcgtccaag tggtgtttgg cgttctacat taatcgtacc
    16081 tgcaccatga catttatgac aagtagttgg ttgtgtgcct ggttttgcac cgttcccacc
    16141 acaagtatga cagatttctt cccgattata cttgatttct ttttctacac caaaaatcgc
    16201 ttcttcaaaa gacaagtcga ccgtatattg caaatcagca ccttgacgag gcgcatttgg
    16261 atcgactgac cgaccgcctc ctccaaagaa agagtcgaag atatcttcaa aaccaccgaa
    16321 gccgccacca gagaagccgc cgaatccacc aaatccgcca ccagctccac cagcaccgta
    16381 atttggatct gtgcctgcat gcccgtattg gtcatatgca gcgcgttttt gcgggtcact
    16441 taaaatctca tatgcttctg atacttcttt gaatttttct tccgcatcag gttctttatt
    16501 gatgtcagga tggtattttt tcgaaagctt acgataagct ttctttattt catcttctga
    16561 tgctcctttt gagagcccta agacttcata ataatcacgt tttgttgcca taggctttcc
    16621 tcctattggt tctcatcatt ttcacgtcgc ttgttatcat atcatatttt gtttgaaaca
    16681 aaaaccgata cttcaaacta tttttcaaaa gaaaaagcca aagtagcaac tttggccttc
    16741 tctcgaaatt cagtgaactg attatttatc gtcgccatcg acttcttcaa aatcagcatc
    16801 aacgacatca tctgctccgc cttgagctgc ttctgggttt tcttgagctt gttgttgtgc
    16861 cgcttgttcg tatagtttaa cagtcaagtt ttgaacgatt tcgttcaatg catcgcgttt
    16921 agttttcatt tcttcgatgt tgtttgcttc aacagcggct ttcaattcat cgcgagctgt
    16981 ttctgctttt ttcacttctt cttcgtccac tttgccttct aattctttca atgttttgtc
    17041 cacagagaat agcaatgcat ctacatcatt acgtaggtca acttcctctt tacgagcttt
    17101 atctgcttct gcatttgctt ctgcatcttt taccatacgt tcgatttctt cgtctgttag
    17161 acctgaagaa gatttgatcg tgattttttg ttctttttgt gtacctagat ctttcgcaga
    17221 tacatttaca atcccgtttt tatcaatatc gaatgttact tcgatttgtg gaataccacg
    17281 aggagctgct ggaatatctg tcaattggaa tcttcctaga gttttgttgt cagcagccat
    17341 tgggcgttca ccttgtaata catggatgtc tactgctggt tgattatctg ccgcagttga
    17401 gaatacttgt gatttacttg ttggaatcgt tgtattgcgg tcaatcaatt tagtgaacac
    17461 tccacccatt gtttcgatac ctaatgacaa tggtgtaacg tcaagtaaaa caacatcttt
    17521 cacatcacca gtgatcacgc caccttggat agcagcaccc atagcaacta cttcatcagg
    17581 gttaactgat ttgtttggtt cttttcctgt ttctttacgt actgcttcta caactgcagg
    17641 gatacgagta gatccaccga ctaaaataac ttcgtcgatt tctgattgtg ataaacctgc
    17701 atcttttagc gcttgacgta ctggtacttt tgtacgttct actaagtcag ctgttaattc
    17761 atcaaatttt gcacgtgtca atgtcatttc caaatgcaat ggtccagatt ctcctgctgt
    17821 gataaatggc aagctgattt gtgtgcttga tacgccagat aaatcttttt tcgctttttc
    17881 agcagcgtct ttcaagcgtt gtaaagccat tttgtcttga gacaagtcaa tgctgttttc
    17941 ttttttgaat tctgctacca tgtagtcgat gattttgtta tcaaagtcgt ccccacctag
    18001 atggttgtcg ccagctgttg acaatacatc aaagacaccg tctcctagtt caaggataga
    18061 tacgtcaaaa gtaccgccac caaggtcgaa tactaagatt ttttcgtcgc gatctgtttt
    18121 atctaaacca taggcaagag cagctgcagt tggttcattg acgatacgtt ctacttctag
    18181 accagcgatc ttaccagcat cttttgttgc ttgacgttgt gcatcattaa agtaagctgg
    18241 cacagtgata actgcttttt cgactttttc acctaaatag tcttctgcga atcctttgat
    18301 atattgaagg atcattgctg agatttcttg tggtgtatat gatttacctt ctacatctac
    18361 tttgtaacct gcttcaccga tatggcgttt gattgatgaa atcgtatttg ggtttgtcac
    18421 agcttgacgt tttgcaactt cccctacttg gatttctcca tttttgaatg atacaacaga
    18481 aggtgttgta cggtttcctt ctgggttagc gatgattttt gcttcgtttc cttccaatac
    18541 tgctactgca gagttagtcg tacctaagtc aataccgata attttactca taattaacgt
    18601 ctccttcttt aattgaaagt gtaagtttta tatccgcaca attattgtgc aacaatgacc
    18661 atggttggtc ttaacacgcg atcatgtaat ttgtagcctt cttgtagtac ttctacaatc
    18721 gtatcggctg gtgtatcttc agtcgctgga acagtttgta ctgcttgatg aagattagga
    18781 tcaaaggctt ctcctttagc agggattttt tcaattcctt cttcttccag tgcgttacgc
    18841 aaactttcca agaccatttc cacgcctttt ttcaaacctg ccccttgatc atctgacact
    18901 tctgtggcta atgcacgttc caggttgtca attgatggaa gtaatttttt ggctaaatct
    18961 tgtgaacggt atttttgtaa ttgctcacgc tcattttttc ctcgatttgc catatttgcg
    19021 atttcagcac gagcccgcaa gtatttatct tccatttctt ctaactcagc ttttaatttc
    19081 gctgtttcga attcttccgc ttcagcttct gaaatacccg aatcttctgc agcttcgaca
    19141 tcgatttccg gttctggctg agcatctgtc atttctttat ctagttcttc ttgattttct
    19201 ttcacgatac agtcttcctt tcattattca gtcagctgtt tgattgctct tactaagaaa
    19261 gagagaacga tcagctgcaa atagcttcgt tattcacatt acgagtttga caagtcaagc
    19321 gaacggtagt aatcagcaag tttagctgct agttctcttc gataaacatc gactagaccg
    19381 aacatccgag aataaggcat actagtaggc cctaacaagg ctattgtacc tcttccatga
    19441 ccatctatct cgtaacttgc ttgaatcata ctcatattct gcaataaatc attgcctaat
    19501 tcagcaccaa tcttgatatg aatattgcta tccatcggag tgatcatttg tgtaagtccg
    19561 tctgagtctt tcataaaaga aaacatcgat ttgaactgat caagatcttg acggggctca
    19621 taatccaaga gattcatttg tccactgaca taaaccttat cttcaaaaac atgaccaaga
    19681 acggtgtcaa ataactgaga catcccttct gtcgtttgga aatatttatg aagaatcatc
    19741 ggaatctccg tacgaagttt atggtacacc gtcatcagag gatctccgac tagacggtcg
    19801 tttacaatct gtaccatttt ttctagttcc tgacttccca tatattcggg aagagtgaac
    19861 acttggcttt ctacattccc catatctgtt acgacgatag caataatctg tcgattattc
    19921 aagggtacga tacgaaagcc agtcagtcgg cggtctttta tttcaggtcc caatgaaaaa
    19981 gtcgtatagc ttgtcagctc agataatatc tcagcagatt gcttaataat ctcattgatt
    20041 tcatgaaatt ctctgcctag agactgacga atcagcagca aatcatcttc tgctacgcga
    20101 gcaggtttta acaaatgatc cacgtaataa cgatagccta aagcagatgg aatccgccca
    20161 gaagaagagt gagtcttcaa cagcaatcct tcatcttcta atgctttcat atcattgcga
    20221 atcgttgctg agctggcctt gactccctca gacatcaatg ttttggaacc aacgggcacg
    20281 ccactactcg tatagtgttg gatgatcaga cgtaaaatgt cgttttgtcg ctttgttaac
    20341 atcttcctca cctctcatta gcactcatat attctaagtg ctaatccact tataaatata
    20401 gcaagaagcc cttctcctgt caagagaaag actccatttt ttagcactct aaacacaaga
    20461 gtgctaattc taaataattg atgtaaatcc agttatatca acgtttcaat agtcctttat
    20521 tgtatttttg aacttttttt atttaaattc agataaaaaa caaatattct ttaaactatt
    20581 tttcaaacaa aaagcgctca aacacattgt tccccaaaaa ggtccctctt ggtgtcaaat
    20641 aaacacggtc ttcttcatta acaagtaacc cttcttgctc taattccctt agcacatcac
    20701 cgtacaccga ttcaattgtt ttttgatagc gatcatagaa atgtttttta gtaatccctg
    20761 ttttttttct tagtcctaag aacatttctt cttctatttg attttttcgt gtcaattttt
    20821 cttcttccaa taccgggcgt tcatttgctc gtaatgggcg taaatagtgt tggattggtc
    20881 ctttgttgcg gtaacgaata tctccaagat aaccgctagc accagctcca aacccaaaat
    20941 agtggtcatt gttccagtac atgaggttat gttgagattc atgtccggag agcccaaagt
    21001 tacttatttc atattggtga cgtccatgct tttccattgc ttcaatcgtc tcttcaaaca
    21061 ttctggtttc cgtttcctga tcaggcagtt cgagtcttcc ttgtcgtacc cagttcatga
    21121 acattgtttt gttttcgagg atcaatgagt acaaagaata gtgaggcaga tccaaagcca
    21181 aagctttttc caaagtatcc agatatcctt ctagtgtttg tcccggtaat gcataaatca
    21241 aatcgatgct tacattggta aaattttctg cttctaaaaa ttgcatagtt tgatacacat
    21301 cttctgcggt gtgtttacgt ccaatttttt tcaataagcg attatcaaat gtctgcactc
    21361 ccatggataa acgattgacg ccatagtttt tcattacttg aagtttttca cgggtcaaat
    21421 cacctggatt cgcttctaca gtaaactcat taccattttg aaaaggcagg atctctctag
    21481 cacctgataa cagtcgatca agttgtttag cagataaaga agttggtgtc ccgcccccta
    21541 cgtaaagtgt ttctaaattc gctgtcggat gactttctag cattattttc atttcattca
    21601 gcagcatttc cacatactca tctactggct gcccttctaa aaaaacttta ttaaaatcac
    21661 agtaatagca gatatgctcg caaaatggga tatgaatgta cgcagaaacc ttgttttctg
    21721 cgggatttat tgtttctatc attgaaattc tttctcctta cgtcgaaaag tcgtgcctgt
    21781 tttcgacctt cttaaaatcg cgttcaagca ccaactcatt gaaataagcc gcatatttag
    21841 aatcaaggaa acctagctct aaaaatgctt gcttattgtc atcggagcta cacggtgctt
    21901 ttacagcctc tcgataaacg gtaatcgcaa agctgacctt cggcattaag tcaaaatctg
    21961 caaaaatatg aaaagctatt ttcacagatt cctccttaag tgctcagacc acaacgcttt
    22021 gcttacaacc tctcatatac gcatactaaa cgtatttggc gaacaaatcc actaaaaaat
    22081 ccaagaatcg acttcttgga tgattttttc tttcattaaa ctggttacct agttatatat
    22141 ctctagggtt gttttcatgt ctgagtgccc gatatgattt atttttcgtt tctataatta
    22201 ctttttgcct tccagtttct tttaatattg tactcaagca agtgggaatt ggttcttttg
    22261 tcatccatgt atgcaaaatg ttataatgct ggtaaacaaa aatgattacc tgtcattttt
    22321 atttagttcg tatttaatta agttgactat ctgtatactc tgtctaaaac ttaagaattg
    22381 ttgttttcgc tgtcgctacc caattttaat gaatgtctgt aggttaaaac tgttgtcctt
    22441 gttatagata gtaaaggaga gcttgaaaat gaaaactgcg attacgtttc tattagagga
    22501 atatgccgat tgggaaggtg cttatattac tagcacacta aaccaaaatg agaaatggca
    22561 aaacaaaact gcttctatcc aatcagaggt cacttccatt ggaggattaa aaacaaaaat
    22621 cgattactta atcgaacaaa tacctgaaaa agttgacctt tttattatga tcggtggcaa
    22681 ttcatgggac ataaaaaatc atcatttata tcaaataatc gataaattgt taaaaacaga
    22741 cgtaccagta gctgctattt gtggggcagt tgattattta gctcagagcg gattattgca
    22801 aggttacaaa cacaccggaa atgcgcaatt tccttggtta gaatcggaaa attatcagaa
    22861 taaagaagat tttatagaac aacaagctgt gaaagatcgt aatttgatta ctgcgaatgg
    22921 aacagctcct atagaattca cagacttgat tcttcaagca gtccattttg aaacagaaga
    22981 aataagagaa aaaacgattc gtctatacaa acttggattc tatgattact ataaaaagta
    23041 cggagatcct ttctcctaat cattctcttc aattaaatac tccttatcaa tagtgtgaca
    23101 agtaatttta ctgatgatta tttgtcacac tttttctgac atttatcaaa gaagatacac
    23161 tctatttata ttcatagaca aggttttacg gattcaaaaa ttcaggatta tgctatactg
    23221 gtattaaaaa aataatgagg tattttatat gaaaaagtta ggttttattt tattgttggt
    23281 ctctttattc cttgtaggat gtacgtctcc tagtaaagaa aatagtgatc catctgcgac
    23341 cacagacaaa agttcaatca gacaaaccag ttcagctgaa aaatcaagta ctcagaaatc
    23401 agcttctact gaaacgaata catcttcaac aagcagtaaa gtctcagagc ccaatacttc
    23461 ttctacatct agtagtacag ttgagacaaa tatttcttct tcaacaatag acacaacctc
    23521 ctcacagtct tctcagtcaa tagatccgtt agcaggatat tctgatctac aaattgagta
    23581 cgcacgtgtt tggctagcta ttagaggtaa tacttttaga gatacatcgg gtgatttcga
    23641 attacatgtt attcattatc ctgcaggaac acctatcgcc ccttatgatg cagggagtgc
    23701 agtctatccg aaagatgtgg ttatgctcac tgggaaatac agttaccagg gagttttagt
    23761 gtattcaagt aaccatgacg ggacaatcac tagttatcca gttccttcac attggcaaat
    23821 gcctgcagat caaacatctt atccagcgtt cattagagaa acaactcaag aaatcattga
    23881 taatgcatct gtagtagcta ttcctacagg aaatcccaat gacgtgaaac agttaatcga
    23941 tgtgacagtt atagataatt agactttcta atatgttata caaaaagaag ctgacagcat
    24001 tttgtcagct tctttcactt tcttttctat ttagatttat tggtctttga tagactccaa
    24061 ggcaggaaaa aaatttttaa ataaacgttt gcaaaacagt cgcttttcta tcttctaccg
    24121 ctacttctgc ataggcacca ttaatcaatg tcagttgtgg cggtacatga ccacaatcaa
    24181 tatcatacaa gatcggtagt tttaattcct ttgacagatc ttcgtataca tccttcattt
    24241 tataatctaa caaatcctct gccgcacttc taccgaacat gatccccgag ctgccatcaa
    24301 accagcctgc cagtttcatc tgaacaagtg atcttcttaa atcaactgac gatagctcac
    24361 agttttctag aaaccacaag atcggctttc catcattata ctttctttta aaagattgga
    24421 catctccata aggagtaccg attaaatgcc tgatcgtatc aatacaacca cctaataatc
    24481 gtcccttcat ccttactgca tctccgctga ctgattgcca atttgttggt tcagtgagat
    24541 ggaaaacaga agatgatgaa tgctcatggg gccacacttt ttgatacttt tcagaagatt
    24601 gctgtagaat cgattctcct tctcttgttt tcagtacact ttcccacatt gctgtcgttt
    24661 catccaagta ctctcctctt aaatcaataa ggttagttcc atgtgcagtg gctatccctg
    24721 tttttaatgt gacagcaagt agcaacgtac taatatctga ataacctact atccattttg
    24781 gttgtagagc atcaaaatcg acaaaatcga gaatctcaat caataattct cctccccaag
    24841 tcggaaagat catttggata ttttcatctt gcatcatttg attgaattca tctgctcgtt
    24901 ttcttgctgg acctgatttt tccttatctt gcgtccaaag cgtctttcca gtaactactt
    24961 ggaaagattt ttcttccatt ctctttaaag ctgattcgag taacgaatgg tgttcctctg
    25021 atacgcctga ggatggtgca gtaaccccaa tcgttgcatt tctttctaat aatggataac
    25081 ttatcatgtt gcttcacctc caagtatgca ctattttact tggttaattg tttttttcgt
    25141 ttgccggcat caaaaatatc ggcatctcaa gtagctggat gattttgttt aagtcaaccg
    25201 tttggatgac catcgtttga tctggtctac ctggtgcaaa aacaagtttt tcatttgata
    25261 agagctcttc atcaataata atcggaatct gtttttcata accaaacgga gaaacagcgc
    25321 cctgcttttg tcccgtttgt tcttccatgc gttctgatga aacaatagaa agccttttac
    25381 cgacgatctt ccctaatgct ttgaaatccg tttgtttcgt agtaaaagtc agatatacgt
    25441 agttattatc ttttttatct tttaagaata agcttttggt ctctgtacct gtaaatcctt
    25501 gttctttctt tattaccaaa gcatcttcat ttgtataaat ggctcgatga tgatacaact
    25561 caaaatatat attttcgtgt aagaatttct ttgccaagtc aatcattttt atgtctcttt
    25621 tctattttat tatttgagac atcttatcat ataaatatag cggttacaac attacttgtg
    25681 ttttgttcgt tttttataca taactattgt ttttctggta aaataaatca aaccacttaa
    25741 tggaggcgtt ttagaatgga aaaatttcag ccgatcatca atcaaatcaa agaaaaagaa
    25801 catcaacaaa agatcaaaga cctttttgct aagataacag aaacgtatcc ccagctagac
    25861 acaactgtaa aatggaacca acctatgttt actgaccatg gtacctttat tattgcattt
    25921 agttttgcta aaaatcattt ttctatcgca ccggaaaaag cagcgattcg tgcccttgaa
    25981 aaaaatatcc aagaagcagg ctacgtatat acggataacg tcatcaaagt tccttggaaa
    26041 agtgtaatca actgggaatt gattgaacaa ttgatttcat ttaatattga agataaaaaa
    26101 ggtcatgata aattttggcg ataaagcttt atacgaataa accttttact ggcgaggaaa
    26161 ctattcatca ccttatacag gttttgttca atcctatcct ttttatcatc cctggttaaa
    26221 cacttaattg acaaagtatc tgctattttt ataaggagtc tttcttgtgc tgaaaaaaat
    26281 cactgattga atggctttga attttctact tagtaaaatt attttttcag caattttcct
    26341 gcaaaacttt tgaataagga aaaaatacga gaaaatatca gtgatctttc ataatactgc
    26401 ccattatttt caggtatatt taaatataat atcactttag gagagaacga atttctttag
    26461 agaaataaaa gttagaattt tctgacttga tttttacaat gaccttaatt aaaatcgttc
    26521 tttctacatt tcgtgattaa gcaaatatca actaagtcag tagaaccatt cacataggag
    26581 ggtgaacatt gaaagatttt tactcagtaa atgaattagc cgaacaatta ggtgttacta
    26641 cacgttctat cagaaattat cttcatgaag gaaaactaaa aggaacaaag gttggcgggc
    26701 aatggaaatt ttctgaacgt aatctttttg agttcctata cggagatcaa gcagaggaag
    26761 cagcaaaaga aatgcagcga ttcatgctaa atgcaccaat cacgatgcgt ttcaaccttc
    26821 aatatcgtga ttttacagca atcaatcaat tccgagaaca attagtccag taccataacg
    26881 atgtttacgc aaataaaaaa gaccgtcttt tacaatacga tctttataaa gacaatcatg
    26941 cggagatact aatcggtggt aacttcaatt atgtcgtaaa ctttagtcaa tggatcaatg
    27001 aaaaattatt gatccagaca gatatttccc ttgtatctta aacaattaaa aagcgctaga
    27061 aattccagag atgttggaaa ttctagcgct atttttttat aagtacttac tgtttggttc
    27121 aaacaaatat ttttttaata aatgaaattt taccatcttt atcctcaaat aaagaccgtg
    27181 accatgcttc ctactacgat cagcaataat ccgataatcg ttaatttttt ttcttttggt
    27241 gtcttatttt catgcaggat gtacataccg cctaatgtag agataactac gtttagctgt
    27301 gtaatgatat atgcagtagc aaccccattt tcatttgctg aaaaaatata agccaatgct
    27361 gacacactaa agatggctcc aacgatcgtt gctttccaac tcttgtcttc agaaaatgcg
    27421 gttggtttac ggaaaatcac ataaatcact gcccctaaaa agacaccgat catttgcgga
    27481 aagaaaatgg aaagtccgct tgcatcaact agtttaggca atgcactgta tacccaataa
    27541 ccaatacttg ttgatgctaa tagactaatt ccttttgtga taccgccttt ttttccagga
    27601 tcatcactga ttgccgttaa agcagaacca aatacaagaa gaatgattgc tgctgctcca
    27661 atgattttcc ccatacttcc agaccattct ccaaaagcga agacaccaat caacgaggtt
    27721 ccgactaact gtaatgcagt cgaaattggc atcgttttag aaacaccaat caagttgaaa
    27781 gatgtatact gtcctgtttg tccaataacc caaaatgcac cagaaagtaa actaatcaaa
    27841 aatgtagtca ctgaaatacc agctggagaa gcgaataatt gaacgagtaa gccgacaagc
    27901 accgctccaa taccagttcc taatatctca tttgttggtc tgccaccaat ctttttcaaa
    27961 atcagtggct ggatccccca gccaactgcc gggatcaatg caatcaataa acccattttt
    28021 attcaccctt atccttttcc accttggaaa gaaggatata aagtcatccc accatctgca
    28081 aatagtgtaa tacctgtaac ataactggat tcgtctgaag ccaaccatgc agctgtcgcc
    28141 gctatttctt ttggttcacc tacgcgtcct agtgggatca tactttccaa gagttctttt
    28201 tgttcaggat catcaaattt tgccgcatta ataggtgtac ggatagcacc agggcctata
    28261 ctattgattc tgattccctt cggtgcatac tccattgcta ccgtttcggt aaataatttt
    28321 accccgcctt ttgaagcagc gtaatgagca aacgttggcc atgggatctg ttcatgaaca
    28381 gaagacatat tgatgatatt tcctttttta ttattctctg taaagtaatt taaagccgct
    28441 ttcgttccga taaaaacgcc agtcaaattg acttttaaca ctctttccca atcttcaagg
    28501 ctaagttcat gggttggatg ctgattttcc atcccagcat tattaaccca aacgtccagt
    28561 gtcccaaaat tttctaaagc cgtatcaagt aaacgctgta ttccttcttc agaactgaca
    28621 tctgcttgta cagagactgc ttttccgcct gcctcttcga ttgctttgac tgctttttct
    28681 gctccttctt tatccgagtt atagttgaca actacattca ttttttcttt cccgaatcgt
    28741 tgagcaatcg ctgttccaat cccctttgag ccaccagtta ctactgcgac tttgttttct
    28801 aattccttgt acatagtacc cactcctttt tcatacctaa acctgttatt acggataact
    28861 tttatttccg ccttaaagtg agtgtacacc aagatatata gtctaaagca aatgatacgc
    28921 tctagcaatc gttttcatga attgctagtg tccttttatt tctataaaga aagaggttgt
    28981 gacagaagtg gtcagcttca agaaatttcg gcttatttcc gaagaagcta cttctgaaaa
    29041 accatttatt tggaaaaaaa ggctgtgaca agacttttgt cacagcccct ttcctgggct
    29101 tatttatcac ccattttatt gtaattgcta gttatacata aacaagagat tttatgcata
    29161 atgttttatt gctgagaaaa atattcagct gttgcttgtt catcctcctt gagttgttca
    29221 atcaattcct ctacactaga aaatttcact tgatctctta aaaattgatt ccagcgtacc
    29281 gcgacatgtt caccatagat atcttgatta aagtccaaaa tatacaactc tacagtcagc
    29341 tcccgtcctt caccgaatgt atcattatgt ccaattgaac ccatggctgg ataccatgtt
    29401 tccccaattt ttatttccga cacataaaca ccttctttag gcaaatgaac cgtgctcttt
    29461 actttgacat ttgctgtagg gaatcctagt agtctgcctc gtgcatcgcc atggacaact
    29521 accccttcta cctcatatgt gcaacctaat agttttgctg cttcttttac ttgtccgctg
    29581 tctagtaatc cacgaatacg ggtagagctg attttttcac cttgttcttc ttctttagaa
    29641 actgtgacga ttttgaatct tcctttggca tattcaggaa aatgagcgac atctgcaatg
    29701 tcttttggtc catacgtata atcaaaaccg gaaacagcat attttgcatg taaccctacg
    29761 atgtattgat caacaaattc ctgcggtgct aaatgagcaa aagaagaagt aaaatcaact
    29821 tcatataaat aatctatacc caaagcggcc attttttctt ctttttgctt aaggctagtc
    29881 aaatatttca cgctgctagg atcgatcttt ttaaagacaa tagatggatg ctgattgaaa
    29941 gtcatcaatg ctaatttcaa tccttctttt tgtgcaattt ccatacctgt ctttatgact
    30001 ttctggtgtc cgatatgcac gccgtcgaaa aatcccagca ctaaaacgac atcgtcttct
    30061 ggaatttgtt ctgattggta aggatgtctg atcttgatga tattcattcg ttattcctca
    30121 tttctcaata ctttactagg ttttaatttg tttttttctt tcgggtttgg ctgataaata
    30181 ctgacaactt ttcctttata aaacaaagca atttcttctg acggcatttc tgggagcgca
    30241 aatacagtat aatcaagtct cataccgttt tttactttct gccatacttc atcagtaata
    30301 tctactcgtt tgaaccgttc cactcctttt tctattggca gcaaacaatc atcgattgtc
    30361 ccatttttca cagcttgagc tacttcttct aatgtcagag catccttctg ggacataccg
    30421 gcacttgctg ttcgggtcag atcagacatg tgcgcagggt aaccaagttt cttgcctgta
    30481 tctacagcta atgtgcggac ataggttcct ttcccgcatt ctacttcgaa gcgccaagaa
    30541 atggttcctg tcacttcgtc tcttgtcaat tcacttgtcc ggataaaacg gtaaatctga
    30601 gcctttctaa caggacgctc cactgtttca ttattgcggg catattcata taaccgctta
    30661 ccgttgactt ttaccgctga gtacattggt ggtatctggg tgatctcacc aataaatgaa
    30721 gccatcactt catctatttt tttttcagat aaagacatat ctactggttt tgtttctacc
    30781 cgttctccgg atcgatcttc cgtcgttgta ctgaaaccta gagttatctc gcctacatag
    30841 attttcccag aatctgtcaa atattcgata acttttgtgg cttttccaat acaaatgggc
    30901 aacacaccgt ctacgtctgg gtcaagcgta cccccatgtc cgattttttt cgtatgtaag
    30961 atttttctta atttgaatac gcaatcatga cttgtcattc cgcgttcttt ccataaaggt
    31021 aataacccgt ccatgtcttt tctccatttc atttttgatt taactgaatt attataacac
    31081 attgcatcaa ttactgacga atttgacatc ataatgaacg gtttttattt ttcggcaata
    31141 aaaacgtaac aaaattcatg ctcacgtaaa tagaaatcat gttatgcttt tttaaaggag
    31201 ggaatagaat ggaagatttt ttctttgcac catttgattg gatttatggg tggcttacag
    31261 gtaaatagtt tggttttcac ttcccaaaaa cgaaaaaaaa gactggccat tcttccagtc
    31321 ttttttccgt tttttttatt tattttttca gtttcttaat caatgtaaaa aattctattt
    31381 tgcgggatcg attttatcat tttattggtc aagatttttc catttccaat ccaatacttc
    31441 tggcagatca ataccatttt cacgaatata gttttgatgt tcttgtaatt tctgatccat
    31501 ttttttcgca aaatcttctg cttgacttcc tttgattttt aaagctgcgt cttttgccaa
    31561 atgataacga tcaagttcat ttactacacg catgtcaaat ggtgtcgtga tatctccatt
    31621 ttctttgtag ccatgaatca ttaaatgatg gttatgacga tcaaagaaaa tcgtgcggac
    31681 caatgtttca taaccgtgga aagcaaataa gatcggtttg tcttttgtaa aatattgatc
    31741 aaattcttca tcgctcaacc cacgcggatc ttgacttgga tgacgtaatt tcaataaatc
    31801 tacgacattg atgaaccgaa tttttagttt tgggaattct tccagcagca aagttacagc
    31861 tgctaatgct tctaagttag attccgttcc tgcagcagca atgaccaatt caggttcctc
    31921 ctcttttgct gtcgaagccc aatcaatgat tttcaagcct tcattcacta agacggctgc
    31981 ttcttctgct gagtaaaatt gagcacgcgg atgtttggaa gagatgatca aattgatttt
    32041 ttctgacgag cggaatgctt tatccatgac tgcgagcaat gtatttgcat ccgcaggcag
    32101 atattcacgg atgaactccg ccttcttttc tgccaaatgt gtcaatattc ctggatcttg
    32161 gtggctatat ccattgtgat cttgctggaa gactgttgag cttgcaatga gatttagcga
    32221 aggataatca tttcgccaag aaaggtcatg tgatttccgc atccatttga agtgctgagt
    32281 caacatagag tcaaccactc gcaagaatga ttcgtaactt gcaaaaaacc atgccgtcca
    32341 gtcagaacgt acccttctaa gaacccttca tcttggtgtt ccgataattg actgtcgatg
    32401 acgcgaccag atggcgaaag ccattcatca ttttctggtt cgattttttc catccattgg
    32461 cgatttgtcg ttttgaaaac ttgattcaat cggtttgatt ttgtttcatc tggtccaaat
    32521 atacggaaat tttctggatt tttctcaatg atctcgcgaa cgaatttacc aagttcggtc
    32581 atatcttctg ctttaacgga gcctggtttt tcaaattgca aagtatattt tttccaatct
    32641 ggcataataa gcggtcttgg atcgattcca ccattggtga taggattttt tgccattctt
    32701 tggtcaccag taggcgcgat ttctctgatt tcttctaaaa tacgtccttg tgcatcaaat
    32761 aatttttctg gttcatagga tttcagccaa cggagtaaag catctgcatg atccatatgt
    32821 tcttgatcta caggaatcgg tacttggtgt gctcggaagg ttccttcaat tggttccccg
    32881 tcccattctt ttggaccggt ccatcctttt ggtgtgcgta cgatcaatac tggccatttt
    32941 ggcatcacga catctgttgc tgtcttcagg cgagcttctt tttggatgga tttaattttc
    33001 tcgattgctt gatccatttt ttcagccata acaggattta atttttctga atcatttcct
    33061 tcaataaaga aaggttccca gccaagtccg ttaaagtaat ttgctaattc tccatctgat
    33121 ttacgtgcca agatcgttgg gttggctatt tttgcaccat ttaaatgaag gatcggtaaa
    33181 acagctccat ctatcactgg attgataaag acatttgaga accagcttcc agctaaagga
    33241 ccggtttctg cttcaccatc accaatcacg acagcagaga ttacttcagg attgtctaat
    33301 acagctccga ctgcatgtga aagagagtat ccaagctctc cgccttcatg gatagaacct
    33361 ggtgtttctg gtgctgcatg agaagcaatg cctccaggaa atgaaaatcg tttgaataat
    33421 ttttgcattc ctgtctcatc ttccgttact tctggataga tctctgtgta acttccatct
    33481 aaataagcat tcgataccat gacttgtccg ccgtgtcctg gcccttcaat ataaaacatg
    33541 ttcaaatcat atttattgat gactcggttc aaatggacat aaataaaatt ttggccagga
    33601 atcgttcccc aatgaccgat tggatgtttt ttgacatctt ctggtttcag tgtacgtctc
    33661 agtaaggggt tatctttaag ataaagttgc ccaacagaaa gataattagc agcacgccac
    33721 caagccgtca ttttatcaaa gtattcttta ctagaataat ccatacataa cactcctttt
    33781 cattattact cttattctag cgcagatact cccaattgaa acctaataag cacttctgtt
    33841 catttgtttg gattttatat ctagccaaat gaacaggtat aaaaaaccac gaaatttttc
    33901 ctttcgctga cgttttttac attgatccaa aaaacttgtc gttatcacaa tggctgttcc
    33961 gcagttcatt tcgctcctca tcatgcataa tttgctgaat gacgctaggc cagttaacac
    34021 attgttggaa aactggggct ggatcgagca attcctttcc tgattgatcc gttttatgta
    34081 aaagtctctg ttatctttgt caatatgtga attggtatcc cagctactat gctaatttct
    34141 acagggatta tccaaaattt ggatcgtgac caaatcgaag cggcgtaatc ggaaatatca
    34201 ataacttcaa cgtgattttt cttttaactg tcaacggacc gacaaactca aacttttatg
    34261 gtgcaggttc cactgatctt ctagttactt ggctatacct gactgtcact aaaatagatt
    34321 acaatctagc ttccgttatc ggtatcttga tctttatcta tatgcaaaaa tttatatact
    34381 atagaaaaaa gaaagtcgaa ttttttactt ttctttttct cttttttggt tagctgacac
    34441 gctaccagac taaagtcatg gaactcttag gtaatacgtc ttaatgaaaa caaaaaaatc
    34501 ccgcaacccc tgatttcaca ggatttgcgg gattttttga ttagtctttg ttcaagtcgc
    34561 gaagcaattc atcgatatgg ttgccataga caactgattc atcaagttca aagaacaatt
    34621 caggtgtctt ataaatactt agattgtgcc ctaactctcg acgaatcaat cctgatgctt
    34681 tattcaatcc atcttgtgct gttttcttat ctgatgctaa gtcagataaa atgctgtaat
    34741 agatcgttgc ttgctgtaag tcccccgtaa ctttcacatc tgtgatcgtt acgttctcca
    34801 cacgagggtc acggaccttt ttgcgcaaaa tatcattgac ttcttttaag atttcttgac
    34861 cgactcggcg gtcacggtag ttagccataa tgattggcct ccttttatca gtttttagtc
    34921 atttttgact tcttccatga taaatccttc aattacgtca tctactttga tgtcattgaa
    34981 tttttcaatc attgcaccac attcataacc cattttgact tctttcacgt catctttgaa
    35041 tcgtttcaga cttgcaagtt ggccttcata gattacgatt ccgtcacgga ttacacggac
    35101 accgctatct ctacgaatat atccttctgt tacataggca cctgcaatcg taccaacttt
    35161 agaaactttg aaggtttcac gaacgatcat ttgaccggtg attttttctt caaattcagg
    35221 gtctagcatc cctttcatcg cagtttcgat ttcttcgatg gctttataga tgatacggtg
    35281 aagacgaata tcgacttctt cagtatcagc ttgcactttt gcctgcggtg taggacgaac
    35341 gttgaaaccg atgataatag cgttgctggc tgctgctaag gttacatcac tttcattgat
    35401 tgctccgaca gctgaatgga cgatttttac acggacacct tccacatcaa tcttcttcaa
    35461 tgatgcagcc agtgcttctg ctgaaccttg tacatctgct ttgatgatta cgttaacttc
    35521 tttcaattcg ccttctttca ggctttcaaa taagttatcc aaagtcacac ggcttgtggc
    35581 agcacggttt tctaataatg cacgtttcgc gcgttcttca cccgctgcac gtgctgtctt
    35641 ttcgtcttcg aagactacga aacggtcacc tgcttgaggt acatcattca agccagtgat
    35701 ttctactggc gttgcaggtc ctgcttcttt atcgcgacgg ccaagatcgt ttgtcattac
    35761 acggacacgt ccgtaagtat taccgacaac gattgggtca ccaacatgaa gtgttccttc
    35821 ttggacaagt aaggttgaca caggaccttt acctttatca agacgtgctt cgatcactgt
    35881 tccgattgcg cgttgtgttg gatctgcttt taaatcttct acttctgcga ctaacaggat
    35941 catttccagc aattcttcga tgttttggcc gaatttcgct gagatctcaa caaagatcgt
    36001 ttctccaccc catgcttcag gaattaactc atactcactt aattcttgca taacatgttg
    36061 agggtttgca cctggtttat caattttatt tacagcgacg atgattggaa cgcctgcagc
    36121 tttggcatgg ttgattgctt caactgtttg tggcatgacg ccatcatctg cagcaacaac
    36181 taagatcgtg atatctgtga tacttgctcc acgtgcacgc atacttgtaa aggccgcatg
    36241 tcctggtgta tccaagaaag tgattggttt accatcaata tcgatctggt aggcaccgat
    36301 atgctgtgtg attccgccgg cttcgccgct tgttacacgt gaatggcgta atgtatccaa
    36361 taaagttgtt ttcccatggt ctacgtgtcc cataatcgtt acgactggcg gacgtgagac
    36421 taatttatct ggatcgactt cttctgcctc gaagaattta tcgatatcag caatgtcaac
    36481 ttggactttt tcttgtggtt ccatgccata gtccgtcgct aacagttcaa tcgtatcttt
    36541 atccagtgct tggttttgat tgaccatgac accaagcata aataattttt taatgatttc
    36601 tgctggctca cgatgaattt ttttcgcaat atctgctacg ttcatacctt cagtatactc
    36661 taatacatct ggcaattcac ggaatttgcg tggtggtaca gctggtttag ttgattgttg
    36721 ctgtttgcct tttttacctt ttttattata acgattacgg ttattattat taaatttgcc
    36781 gcgattgtta ttgtttggtt tgttgttatt gttagtacgg ttttgattgc cgccttggct
    36841 cgttccttga cgattttggc tgctggtatt tcccgtatta ttttggtttg ttgttggttt
    36901 ttttccttga ttcacttgac cgctcccttg accgcgatct tgataactgc ggttattttt
    36961 atttttttgt tgattgttcg ttgtttcttt tttacgttga tcgttcggct gttgattggc
    37021 tggttttctg cttgcttgtt gtgcagcttg ttgtttcgtg cttgggtttt tgaacgcttg
    37081 ttgcaatttc ttctcatcat tgctagtaat cgcacccata tggtttttca catcgatgcc
    37141 aagttgttgt gctttttcca cgacgtcttt actagatttt ttgagttctt ttgctaattc
    37201 atagattctt ttgttgccca tgcaatcacc ttcctaacct tcaattagct ccttggtctt
    37261 cttcgcaaat ccggcatcca ttattccgaa taccttgcga ggttttccga tcatctgcgt
    37321 gatttctgct tcagaaaata gctcaaagca aggaacttca taatatgaac tcttatcttt
    37381 tatctttttt cttgtattct cgcttgcatc ggttgccaca aacaccaatt ttgctttttg
    37441 tttgcggatt tcagcaatgg tcagttcttc acccgtgatt agttttcccg cgcgcatcgc
    37501 tagaccgatg agattcatgg ctttttgccg attaactgta ttcattttcc aaataactct
    37561 ttacgtgctt tttggtgagt gacatagtca agaagttctt gataaaactc atctgttaat
    37621 gttgtttcga gtactcgatc gagaattttt ttatcccaag cttgttgtac ttcttccggc
    37681 tcaagtgaga catacgctcc tcgtccaggc atttttccag ttggatcgat cgatacttcc
    37741 ccttcttttg aacgggtgat tcggaccatc tcttttttag gtttcatttc acctgaaacc
    37801 acggatttgc gtaaaggaat tttcctttgt ttcatctctt tgcctcctta ctaaagttaa
    37861 gcttctggtt tttcttcgac cgtttcattg ttgaatgcaa ttgtttcata ttcatcgtct
    37921 gttaaatctg actgaacgat tgcttcatca tgcagttctt ctgtttcttt ttgttgttct
    37981 tgattttcat aaaattccgt catgtcagat tctgatttga tatcgatttt atggttagtc
    38041 aatttagcag ctaaacgtgc gttttgtccg cgtttaccga ttgctagcga taattggtag
    38101 tctggaacga caactgtgca ggcctttgga tttgtctcat cgaagatcac atccatgact
    38161 tgtgcaggat tcaatgcatt tgcgatgaat acagctggat cttcattcca ttcgacgata
    38221 tccatgtttt cgccttttaa ttcattgacg attgcttgaa ctcgttgtcc ctttggcccg
    38281 acacatgtac cgactgcatc gatatttgga tcatttgagc gaactgccac ttttgaacga
    38341 tcgccagctt cacgggcgat gctgacaatt tcaacgattc catcatagac ttctggtact
    38401 tcttgttcaa ataagcgacg caataggtct ggatggctgc ggctaacaaa tacttgcgga
    38461 cctttagacg tattttctac tcgggaaaca taaactttga ttcgatcatg tggttgataa
    38521 aattcatttg gcatttgatc ttgtttagaa agaactgcct caattttacc aagattgaca
    38581 taaatataac gtttatcctg gcgttcgacg attccttgca tgatgtcttt ttcataagca
    38641 ctgaattcgt tataaataat cgttctttct gcttcacgta cacgctgtaa aataacttgt
    38701 ttagccgttt gcgcagcgat ccgtccaaaa tcttttggtg ttacttcaaa acggatcgta
    38761 tcaccgatct catatgctgg attgattaaa agtgcatctt tcaatgagac ttccaactgt
    38821 gaatccatca cttcttccgt tacttctttt accgcataga catggatctt tccttttttc
    38881 tgatcaaatt ccacttcgac gttttgtgct tggccatagt gacgtttgta tgcagaaacc
    38941 aatgctgctt ctaaagcatc gatcacgatt tcttttgaga tccctttttc agcttctaaa
    39001 gcgtctaacg catttaacat ttctttactc atttttttct gattcctctc cgtgattaaa
    39061 attggatagc taagcgagct tttgcaatat tttttcggtc aaaagtcaat tctttttctc
    39121 gtgtttttat gcgtattttc aatgtaagct gttcagcatc aaatgattga agaaatcctt
    39181 catattgttt ttcgccatct actggttggt ataaggagac atggacataa tcaccaagtg
    39241 ccttttcata gtcgctttct tttttcaatg gccgctctgc tcccggggag gatacctcca
    39301 ggaaataagc ttgaggaatc ggatctggat ctgctgcatc aagtttttca ctcagtttct
    39361 cactgacaaa ggcgcattct tcgatatcga taccgccttc tttgtcaata aatactcgta
    39421 agaaccagct ttttccttct tttacaaatt cgacttcgac aagttcaaaa ttttgttcat
    39481 ctaaaatcgg tgtcaccatt tctgtgactg tttcaacgac gctactcaaa agtttcgcct
    39541 ccttaaaaat taagtgcgta attccattaa aaagagtgag cgaaaaatcc gctcactcac
    39601 agttagtatc attacctata agaactatac catactctgc ttaaaattac aagcagacag
    39661 gaaaacagat gcttatagca tatcgaataa cgataattga ttttcatctg gtagatcttt
    39721 caatacgcca ttgtcgttca tatattcgat caatgttttt gaaactttac cgcgggtggc
    39781 aagatcttct ttggataaaa atggaccgtc ttttcttgct tcaacgattt gtttcgccac
    39841 gttggttccc aaacttggga cagctctaaa tggtgcgatc aacgtatcac cttcaataac
    39901 aaagttaacg gcatccgatt tatacaaatc aatcattcca aatttgaatc ctcgttccag
    39961 catttcattg gctaactcca gcactgtcag ttgattcttt tcttttacgg aagcatctaa
    40021 tcccttgtcc gtgatttctt tcattgcttg tttcacagca tcttttcctt tgcacatgga
    40081 gactagatca aagtcgtctg cacgtacaga aaagtatgca cagtaataca aaatagggaa
    40141 ataaaccttg aaataagcga cacgaagagc catcaatacg taagctgcag catgggcttt
    40201 cggaaacata tatttgattt ttgaacaaga atcgatatac cagtcgggca cattattttc
    40261 tttcatcgcc gatagatagg tttctcgcaa ttcatcaggg atcttgttcc attgtccttt
    40321 acgaacagtt tccatgattt taaatgccat cccgctatct aatccagcat ggatcaaata
    40381 aaccataata tcatcacgac agccgattac ttccgccagt gtcgcatctc ctcgtcgaat
    40441 caattcttcc gcatttccta accatacgtc tgtaccatga gatagtccgg aaatttgcag
    40501 tagttctgca aaagttgtag gatgcgtttc ttctaacatc cctcgaacaa aacgagtgcc
    40561 gaattctgga atacctaatg tccctgtttt ggaataaatt tgttcctgac ttactccaag
    40621 tacttcagga ccggcaaata tccgcatcac ttccgggtca tccgtaggga tcgtttgtgg
    40681 gtcgatccct gataaatctt gaagcatccg aataacagtt gggtcatcat gccctagaat
    40741 atctaatttc aatacattat cgtgaatcga gtggaaatca aaatgagtcg ttttccattc
    40801 tgaattttga tcgtctgctg gatattggat cggcgtaaaa tcatagacat ccatataatc
    40861 gggaataacg atgatccccc ctggatgctg gccggttgtc cgtttgactc cagtagcgcc
    40921 ttttgctaat ctgtcgactt ctgcacttcg aaactgcaga ttattgtccc tttcgtatcc
    40981 ttttacatag ccatatgctg ttttgtcggc gactgtacca atcgttccgg cacggtaaac
    41041 atagtcttcg ccaaacaata ctttcgtata gttatgggct tctgcttgat aatcacctga
    41101 gaagttcaaa tcaatatcgg gcactttgtc tccatggaat cctaaaaatg tctcaaatgg
    41161 aatatcatga ccgtctttgt ttaagcgtgc accacatttt ggacattgtt tttctggcat
    41221 gtcaaatcca gaaccatacg taccatcttc aaaaaactcg gaatattggc actctggaca
    41281 ataataatga ggggctaatg gattaacttc agtgattcct gtcatcgtcg caacaaagct
    41341 agagccaaca gagccacgtg aacctactag atagccatct tcattacttt tatgcactag
    41401 tttttgggcg atcagataaa taaccgagaa gccattacca ttaatagaat tcaactcttt
    41461 ttctaatcgt ttttgaatga tctcaggtaa cggatctccg tacatttgtt ttgcttttgt
    41521 gtaactcaat tctgatattt catcttctga acctggaatc ttcggtgtat aaagttcatc
    41581 tttgactgga atcacttctt cgcagatatc tgcgatttta ttcgtatttt ctactacgat
    41641 ctctttcgct gtttcttccc ctaaaaagtg aaatgcagtt agcatctcat cggttgttcg
    41701 gaaatggaca tccggcaaac tatgacgatt caacggattc gctccgccca tagaattgat
    41761 caaaattttt cggtaaatgg catcttcttc gttcagataa tgtacattac ctgttgcaac
    41821 aacgatttta tccaaacttt ttccgatttc taccaaattt tggataattt cttccaaatc
    41881 atgttcattt tttaccaatt cttgctcgat caaaggcgca tagaccgctt ttggcataac
    41941 ttcaatataa tcatagaatt ttgctcggtt tcttgcctct tctacccctt tttgcatcat
    42001 ggcttcgaat atttctccct tatcacaagc tgagccgatc aatagatttt cacgcatctt
    42061 tttcaattgg gagcgtggta ttcgtgggac gcgttcgaag tattcgacat tcgacattga
    42121 aatcagtttg aacaaatctt tcagtcctgc ctgatttttt gctaaaatcg ttacatggaa
    42181 aggtcgagcc cttttgtaag aatctccttc tccgatatgc tcatttagct gatcatgata
    42241 aagcatgtta tgattgtcca ttgcctcttt cacaaagatc catgctaagt gaccagttgc
    42301 ttcagcatca tagattgctc ggtgatgctg ttccaagcta acaccaaact ttttagataa
    42361 cacacctaat ccgaaacgtt tgaattgagg atacagatat ctagctaatt ccaacgtatc
    42421 aatgactgga ttggccgctt caggaatacc ataccttgcg tagctagtat tcaagaaccc
    42481 catatcaaaa gccgcgttgt gggctaccaa aattgtatct tttgaaaatt caaggaacat
    42541 ccgcaatact tcttcttccg actttgaccc tcgaaccatt tcatccgtga taccagtcag
    42601 atctaccgtt gttcgtgaca atggatgtcc tggatcgata aattcatcaa agctttcgat
    42661 cacatttcct ttatacattt tgactgcggc tagctcgatg atcgtgtcat atactgcaga
    42721 caatcctgtc gtttccacgt caaatacgac gtatgtggct tcactcaatg cttcatgtgc
    42781 atcattataa gcaattggga caccatcatc tacgacattc gcttccacgc cgtataagat
    42841 tttcacacct gctttttttc cagcactatg tgcttccgga aaggcttgcg caccaccatg
    42901 gtctgtaatg gcgatagctc ggtgtcccca ttttcctgct tgtgccacaa gatcagagat
    42961 gctgtttgtt gcatccatcg tactcatatt gctatgaaca tgaagctcta ctcgtttttc
    43021 cccttcagga gcataatctt tgcgaggtgt atgtttgact tctatgatat cttgcgcatt
    43081 catgaccaaa tcacggacaa acgtatcttc ttgaatactt cctcgtacct ttaaccagct
    43141 accgacactg atcgcatcaa atacttgttc atctttttcg ccgttcgaga attttttgac
    43201 gataaatgat gaagtataat ctgtgatttt caaagtcaag atcttgcgtt ttgaacgtag
    43261 ttctctgact tctttgtcga atacgtaccc ctcaagtgtg actcttcgtt cttcttcaac
    43321 gatactgatc attggtgtcg ttggttcatc ggctggaata tttcgtccta gctggatggg
    43381 tccttcaaga gctggcgatt gctcttttct ttcctttttc ttttgttcgt ggacaactag
    43441 actttcagct gcttgcttca taaaggcttc tgcctgttct tgtttgcgtt cttcaaaaag
    43501 tttcaataca cgttccgctt gctgctcatc cacttctggt tcgatacgaa attttgggaa
    43561 tccgtaactg acgaacaact cttctactaa tggcaagtat tgttgcttca aataagaaat
    43621 agctcctgta ctgtcgattg gcaagatgac tttacgatct tttatcacag gtacctgcgt
    43681 tttcaatact tgctgtgcta gtggtgtatc gcattgctga ttttccaaag cttgggccca
    43741 gtaatcttgc aagagctgtt catcgaatgt ctggtcatct gctaagatat tcagtttgac
    43801 ttgtgcaata tctttaaaag cgatagtcag atgctgagtc aatgaccgat atagcataat
    43861 aggcaaaatt ttggaaaaat gtagcgtaaa ttcccataaa cgcgatttac gatggaccac
    43921 tacactatcc atttcaccag cagtaagcag tggatgattt ttttctgctt cctctagttg
    43981 gatttgttcc aacagttttt caaataaatc tcgtttttct gacaaagata tacgcccttt
    44041 ctgatgagaa acaatgcaat tgatcgcatc ttatcctctt ctttttcagt accttttagt
    44101 atactcgttt tttctgagtt ttacattaaa aaattggaac tgcgattcaa atcgcagttc
    44161 caattttaaa agcgaatcat cagattgtca aaaacagctg attcagctat ttttattttc
    44221 tgtgttttaa cgcttattat tcgccttggt tcaagaggat tggtaaagta ttcgccaatt
    44281 cttctttacg tacttcaacc atttcgccag ttttcttgat tttcacttct acgattccat
    44341 ccactgcttt tttaccgaca gtgattctga ttggacagcc aatcaaatca gaatccgcga
    44401 atttcactcc tgcgcgttca tttctgtcat caaccagtac ttcatagcca gcttttgtca
    44461 tcgcttcttc tagttcattc gtcaggcttg tttggtattc gtcttttaga ttcatttgga
    44521 cgatatgcag atcaaatggc gcaattcctt ttggccaatt gattccttgt tcatctgaat
    44581 gttgttctac gattgctgat aataaacgac ttacaccgat accataacag cccataatca
    44641 caaatttttc tcgtccattt tcgtctagta cagttgctcc catagattca ctgtaacgag
    44701 tgccgatttt gaatatatgt ccgatttcga ttcctcgagt aaaggcaaga acaccgttgt
    44761 tgtctggtga aggatctcct tcttgtacaa aacgaagatc ttcataagaa agcacagtga
    44821 aatcgcgttt tggattcaca tttttatagt ggaatcctgt ttcgttcgct ccagtaatgg
    44881 cattcgctaa atcttggaca taacggtctg catatacttt tacgtcatct gaaacaccga
    44941 ctggtccgac agaaccaaac tcagcaccta gatatttttg tgcttcttct tctgttgctt
    45001 cttctaaaaa gtcagcagat aaaaagtttt tcagttttac gtcattgact tcatggtctc
    45061 cacgtaccaa aacaaggaca ggttgttcat ccgcaatgaa caatacagac ttgatgattt
    45121 tttgaggttc aacagaaaag aaatctgcta cttcgtcaat tgattttacg ttaggtgtag
    45181 ctactttctc caattcaagc tgtgtttcat gcgatttttt tgatacatag tagcttgtag
    45241 ccatctccaa gtttgcagca taatcacttt cagtcgaata acagatcgta tcctccccga
    45301 tttctgagat cgccatgaat tcctttgagt ctttcccgcc cattgcgccg ccgtctccga
    45361 tgatcgagcg aaattctaag ccacaacgct tgaatacttc ggtataggct ttttcgtaat
    45421 ctttatatgt ctcgtccaaa ctttcttccg ttgcatggaa agaatatgca tctttcatga
    45481 taaactctct tccgcgaagc aaaccgaagc gaggtcgttt ttcgtcacga tatttaggtt
    45541 ggatttgata aagatttaaa ggtaatttct tatatgaatt gatttcatta cgcatcaaat
    45601 cagtaaaagt ttcttcgtga gttggtccaa ggatcatttt tcgatcattg cggtctttga
    45661 aacgatacaa gtttggtcca tatgtttcgt aacgcccgga ttcttcccaa agttctgctg
    45721 gcaagatggc aggcatcagc atttctaccg ctccgatttt ttcaaattct tcccgcataa
    45781 tgcgtttcag tttttccaat acacgatttg ctaatggcag atatgaataa atacccgctg
    45841 atacttggcg gatatatcca gctcttagca agatttgatg gcttaatact tctgcatcat
    45901 ttggaacttc gcgtaatgtt ggaatcaaca tttttgattg tctcattcta tatactcctt
    45961 tatttcctca aaatagttat cgtttgttat tataccctat ttgtcaactt tttctagaaa
    46021 aagaatcgtt gaatatcgtt ccatgtaact agcaccatca acaacattaa aaatccaaag
    46081 ccagctaatg tcaatattcc ttctttttct tgacttaatg gtttcccgcg aatcccttca
    46141 aaaatattca agactaattt cccgccatct aaggcaggaa tcggaagcag attgacgatt
    46201 ccgagattca ttgaaagaag cgccatcaaa ctaataacag ttgttacacc ttgatttgct
    46261 gcttctgaag acaattggta catcatgaca ggtccgccta atttatctaa actgaaacct
    46321 gtaaatagtg atcctaaggc tttgaaaatt tccaatgaac cgctgaaagc ttgacgcgtc
    46381 cctccaatga tcttatccat aaaacctgta ttcattgggg cttttatacc aagttggcca
    46441 actttttcgc catttgactc tacagatttc ggtgtcacgt cgacagattg cacctgacct
    46501 tctcgttcga tcttaaagtc taatgtttta cctggatttt tcgtaatgac tgttgtcaga
    46561 tcattccatg aatggatttc ttttccgtcc acactgacta cctcatcatt ttcttttaag
    46621 cctgcttctg ccgcagctcc attaggcatg atctctccta cgcgattagt gttcgtcact
    46681 tgaacaccac cttgcataaa tgccagaatg atgaataaca cgattgccaa aataaagtta
    46741 ttcatgggtc cagcaaagtt ggtcagcata cgctgccaca atttagccga ttggaactgt
    46801 acgtcttttg gcgcaattct gatttctgtt ccgtcgtttt caataatcgt cgcatcgtga
    46861 tcaacagggt aggttactac ttctgtttcg tctccattca cataaccagt gatagtcagc
    46921 tcatcttcta aatcataacg gctcaattcc atcgggatag catttgtcaa ttggattttc
    46981 ctacttaaat ttatcttctc tacgatgccg tcagaattta gtaaaagaga aagaggcatt
    47041 cctggtgcca tttctgtttc atcatcccca ttgcctgcca ttcgaacata accgccaata
    47101 ggtaataggc gaagcgtata tgttgtcccg tcttttgctt gatgcccata aattttcgga
    47161 cccataccga tagcaaattc gcggacgagg attcctgatc gtttcgcaaa gaagaaatga
    47221 ccaaactcat gaacaatcac taatatacca aaaacgatga taaatgtcag aatcgttttc
    47281 atagcttttt tctcctttac ttgttttttg tgttttttat aaaacaacga taaaacacta
    47341 taattttacc atagtatacc aaagacacca atagtatcaa tataatgaaa aataaaaacc
    47401 tgcgtctttt atgaaattca caaagaaacg caggtatact tactaaataa tgccaaataa
    47461 atgcatgata ggaaatacaa acagcaaact gtcgaaacgg tctaagattc caccatggcc
    47521 tggtaagata tttcccgagt ctttcacatc ataatgacgt ttgattgcag attcgaccaa
    47581 atcaccaaat tgtccgactg ctgaaaaaat aatcgtcatc agaagcatga caaacatacc
    47641 atgattgaat agttcttttg ctgggtatag caataaataa agaaatgcga ctacaacagc
    47701 actaccgata ccaccgagcg caccttcaat cgttttgttg ggcgaaatat caggacttaa
    47761 cttatgctga ccataacgtc gaccaaccat atatgctccg atatcagttg cccaaacaat
    47821 aaatagacca aatatcaaaa cagccaagct ttctgttcgt gcattgacaa aattctggaa
    47881 acctattccc acatacaagc tgacaagcac aggaaaacct gcttcatcaa tcgtatacgt
    47941 atttttagaa ataactgatg ttcctaaaag aatcataact ataagataaa ataacataaa
    48001 tgtacttaca ttctctggaa gaaagaaaaa ccatctttct tttggcaaaa caagtaacac
    48061 tgcaccaacg gcggataaaa ctccttcaaa acttaagagt gtcaatcctt tcatccggaa
    48121 cagctcgtaa actcctacta ccgctaagat tgctgctgta agctcaaccg ctatgcctcc
    48181 gatccatata atcggaataa acaagactag cgccacagct gctgtgatca cacgttgttt
    48241 catgactgat cccctttctc atcttctgtt ttttttacac cgccgaatcg acggtctcga
    48301 ttttgataag aggcgattgc ttcttcaaga tgcgcaccat caaaatcagg ccaaagcgct
    48361 tttgtaaaat acagctcact atatgcgatc tgccataaca gaaaattact aattcgctct
    48421 tctccacttg tacggatcat cagctcagga tcttgtaact ctttaggcaa gaaacctgtc
    48481 attaaatgct tagcaatcaa ttcatcatcg atttcttctg cgtgtatttc tccttttgct
    48541 acttcttcgg caatttcttg gacagcagtc acgatctctg ctctacttcc ataattcaaa
    48601 gcaaaattca ataccattcc agtattgttt tttgtttgct caattgcacg tcttacagca
    48661 tcttgcgtat gttctggcaa tacgttttca taacccatga catgaacttt gacattttct
    48721 ttaatcaatt ctggtacgaa cgtatcaaag aagtcgacag gtagttgcat gagaaaactt
    48781 acttcatctt ttggtcgctt ccagttttca gtcgaaaagg cataaagcgt caacactttc
    48841 actccaagcc ttgatgcttt ttttgtgact ttctttactg tttccatgcc ttctttatgg
    48901 ccagcaactc gtggcaaacg acggttttgc gcccaacgac cattgccgtc catgatgatg
    48961 gcaatgtgct gaggaattgg tccatcggta ttaaattgaa attcgctttt ttcttgaata
    49021 tatttgtttt tttgcggaaa aaaacgtaac attctttttc ctcctatact taaaagattc
    49081 ccactatctt aagtattata gcaatatcta actgtttttc ctatgaaatc attgtttttt
    49141 tatttaaaaa ctgaataatt ttttcttttt ttaattttta aataaaagaa atataaataa
    49201 aaacagagga agcggaatga tccgcccctc tgtttttatg attttattaa acttccaata
    49261 attctttttc tttatcagca gcgatcttat ctacttcttt aatgctgtta tctgtcaatg
    49321 tttgtacgtc tttttctaat ccgcgcaaat catcttcggt aatatcacca tttttttgtt
    49381 gttttttata ttcatcgatc gcatcacgac ggatgttacg aatcgcgatt tttgcatttt
    49441 ctgcttcttt tttcacatct ttagctaatt cttttctgcg ttcttctgtc agttgcggga
    49501 taactaagcg aatgacattt ccatcattgg ttggactgat tccgatatca cttgccataa
    49561 tagctttttc aacatcttga agaatgctct tgtcaaaagg tgtgatcatt aaaacacgtg
    49621 cttctggaat ttggattgat gccatttggt tcaatggtgt tggcgcaccg tagtagttca
    49681 cagtaatacg gtccaaaagg ctagcgttcg cacggcctgc gcggatttgc ccaagttcac
    49741 gttgaagatt ttgtgctgct tttttcattt tttcttttgc ttctgtcata attgcatctg
    49801 ccatttttat ttccccctta cagttgttcc gatattttcg cctaagattg cacggcgaat
    49861 atttccgtgt tcatttaaat tgaagacaag cagtggaata tcattatcca tgcttagtga
    49921 acttgcagtt gaatccatga cttgtaatcc tttggcaata acttccagat gagtcagttc
    49981 ttcaaatttt actgcatttg catctacttt cggatcagca gaataaacac cgtctacatt
    50041 gtttttagcc atcaggataa catctgcgcc gatttctgcc gctcgtaatg ctgctgttgt
    50101 gtctgtcgag aagtacgggt tgccggtacc gcctgcaaag ataacgatcc gtcctttttc
    50161 taaatgacgt tctgctttac ggcgaatata tggctctgcg atttgacgca tttcaatcga
    50221 agtttgcacg cgtgtcggta cacccacgtt ttccaacgta tcctgtaaag ctaaggcatt
    50281 cataactgta gctaacatac ccatgtaatc agcttgagca cgttccatgc ccatttgtgc
    50341 tccgatttgt ccgcgccaga tatttccgcc acccacaacg attgccattt caacgcccag
    50401 ctcgtgaact tctttgattt cttggatgat ttctttgatt actggtggtt tgattccaaa
    50461 tcctgcatcc ccagctaaag cttctccact caattttaag actacacgct gatatttcgg
    50521 tgtgaccatg ttcattcctc caatattttc tattgccatt ttaccatact tagatgattt
    50581 cgacactcta agactggttt ttcttgtttt gcttaaaaaa agggagcgca caaaccgtgc
    50641 gttcccgtct gttattcaac agaattattt tttcacttgg ctcataactt cttctacaaa
    50701 gttatcttcg cgtttttcga taccttcgcc aacttcgaaa cgtacgaatg ttttaacagt
    50761 tgcgcctttt gatgcaacgt atttttcaac agtcatatca ggatctttaa cgaatggttg
    50821 gtccaccaaa gcgatttctg ctttgaattt cttcaagcgt ccttctacca ttttttcaac
    50881 gatattcgct ggtttgcctt cgttcaatgc ttgttctgta agaacagttt tttcatgttc
    50941 taattcagct tctggaattt gtgattcgtt tacataacga ggattgatag ctgctacgtg
    51001 catagctacg tctcttgcaa ctgtttcgtc agttgttcca tctagaacag tcaatacggc
    51061 aatacgtccg cccatatgca agtatccacc aaatgcagca ttgtcgtctt tttctacaac
    51121 ttcaaagcga cggaagctga ttttttctcc gataacttga gttgcttcga tcaagtcaga
    51181 ttcaattgta cctttatctg ttttgatttt cattgcagct tccatgtctg ctggtttgtt
    51241 ttcagcaaca agttctgcaa tttcttttac taaatcttgg aacatttcgt ttttagaaac
    51301 aaagtctgtt tctgagttta cttcaacgat tgctgcagtg ttacctttca cagcaactga
    51361 agccaatcct tcagctgcaa tgcggtcatt tttcttcgca gcctttgcca ttcctttttc
    51421 acgtaaaaga tcaactgctt tttccatgtc gccttctact tcaactaatg cttttttcgc
    51481 atccatcatt cctacgccag tcatttcgcg tagttctttt accattttag ctgtaacgtc
    51541 tgccatgttt aactcctcct gtgtgttttt ctttttaaaa aaagctgctt caaaggagag
    51601 tgaggcttgt cgcccagcct ttgaaacagc tccatcattc aaaattattc tgctgaagaa
    51661 ttgtcgcctt caacaacatc tacgatttct tcgattgatg ttgctgtgtt ttcttctgtg
    51721 aaatcttctt caactacttg atcttcacct tggtttcctt cgatgaaagc atcagccatt
    51781 tttgaagtaa tcaatttaac ggcacgaatt gcatcgtcgt ttgaagggat tactacgtcg
    51841 atttcatctg gatcgcagtt tgtatcaacc atagctacga ttgggatatt taatttgtgt
    51901 gcttcttgaa cagcaatacg ttctttacgt gggtcaacga tgtacattac atctgggatt
    51961 cttggcatat cagcgatacc gcctaagaat ttttcaagac gttcacgttc tttgttcaaa
    52021 ccagcaactt cttttttagg tagaacttca aaagttccat cttcttccat tgcattgatt
    52081 tgtttcaaac ggctgatacg tttttggata gtatcccagt ttgtcaatgt tccgcctaac
    52141 caacgatggt taacgaagta ttgtcctgca cgagttgctt cttctttgat tgcttcttgt
    52201 gcttgttttt ttgtacctac gaataatgct acgccgcctt cttcagcaac gctcttcatg
    52261 taatcataag ctgcatctac taatttaact gttttttgca agtcaatgat gtagattccg
    52321 tttctttctg tgaagatata tttcttcatt tttggattcc agcgacgagt ttggtgacca
    52381 aagtgtacgc cggcttctag taattgtttc atagaaatta ctgccatgtt tgtttcctcc
    52441 gatttggttt tatttttcct cttcttatct tctaccttgc cggcaaactt tttcaagcac
    52501 ctaccgacaa tccaataaga atgtggattt gctgatgcat aaagcaccct cagctattat
    52561 acagacaaaa gatccgtatc gcaagagcaa atatactttt ctgctcaaca ttcaatagtt
    52621 tacctgaaag ataaaaagaa ttcaagaaaa acagtagatt tctctttgtt tctttattat
    52681 cctttttcca taaaataatt ttaggtataa aagacgaata ttattgggca atccttcttt
    52741 tttttgttat gatacatgtg aaatctttcg taaaggagaa agaaaatgcg caaagctcaa
    52801 agacatttgc tgattaaaca agtcattgaa gaattcccga tccgtacgca agaagaacta
    52861 ttaaacaaac ttgggcatta tggcgttacc gccacgcagg caacgatttc tcgagatatc
    52921 cgtgatctaa aaatcgtgaa ggcacctgat gaaaatggcg tttcccgctt cgtattgttt
    52981 cgcgaaaaag gaactcctga gactaggact gaggaagagg atcggctggt tcaaatgatt
    53041 gaagacattg tattgaaagt cgaacgagtc cactttttga cgatcgtcaa taccttgcca
    53101 gacaatgctc atttatttgc atctgtttta gatgagatca aacctccctc tattgtcagt
    53161 acaattgctg gattcgatac gatcatggtc atctcggaaa atgaggagga cgcaaaacga
    53221 gtagaagaat ttcttcacca tccaactgaa gtatcacttt tttaatcaga gcacgtattc
    53281 ctaatatgga atgcgtgttt ttttattttc aatattttcc ttattaaata aacctttaca
    53341 ctctttacaa aaatacatca ttttttattc aattttttca acaccttgat tgaatcacaa
    53401 taatcatcat ttatattgga tgttaacaag agcagcttct tgctctttgt gtatttcaaa
    53461 aaagaaatga aaggaaatca tatgtataaa ttattaactc ctggaccatt gacgacttct
    53521 gaaacagtga aaaatgctat gctgatcgat cattgtactt gggatcaaga atacaaagaa
    53581 attacacaat ggataagaaa aaaattattg gatcttgctc aagtatcgga aggaatatac
    53641 acaacggtct taatgcaagg tagcggctcc tttggggtag aatcggtatt aagttctgtg
    53701 atcaggccag aagatactgt actgatttgt tcaaacggcg catatggaca acgaatgatc
    53761 aacatgtgcg aatgtctgaa actgaactat accgtttatg aggtatcgga acatcaaatc
    53821 ctccaagctc atgtgattac acagctgatt gaagagaatc cctctatctc cgcactagca
    53881 atggtccata gcgagactac ttccggtatt ttgaataatt tggaagagat tgctgctgtc
    53941 acacaagaaa aagatctttt gtttatagtg gacgcgatgt caagctttgg tgggataccg
    54001 attcgtgtag ccgaactagg gattgatttt ttgatttcca gctcaaacaa atgtattcaa
    54061 ggtgttccag gcttttcttt tgtacttgct aataaagcgg aactagaaaa gcgaaaaaat
    54121 gtgagtcgaa gtttgtcact tgatctatat gaccagtacc gcgtgatgga aaacgagaat
    54181 ggcaaatggc gtttcacttc ccctacccat acggttttgg cttttcaaca agcgctgaag
    54241 gaactagagg cagaaggagg aatcgaaact cgatattcac gttatctcgc caataatcat
    54301 gtactacgac aaaaaatgac agagatgggg ttccagcctt tcattcaaga atatcagggg
    54361 ccattcatca cctcttttct ttaccctaag gactggcgat ttgattttaa tcatttttat
    54421 cagtatttaa agcaaaacgg atttgtgatc tatcccggga aaatcagcga tcatgaatgt
    54481 tttcgtatcg gaaatattgg cgagatctat ccggaagata ttttggaact gacagcaatt
    54541 atcaaagact ataaggagaa cgttaatgat taaaactgta atttttgatt gggcaggaac
    54601 aactgtggac tttggctgta tggctcctgt ccacgctttt agaaatgctt ttctagaaaa
    54661 aggaatccaa ctgactgaca aagaaatccg cgaaccgatg ggaaaattaa aatgggatca
    54721 tatccaacag cttctttccc tcccttctgt caaaaaacaa tggatagcta tttatggaca
    54781 actacctcag gaaaaagagg tggaggaatt gtatcaacaa tttgaacatt atctgtttaa
    54841 agatttggtc aagcattcta ccttgaaacc cgacacattg gaaacgatcg aacggttgag
    54901 aaacgctggg atcaatattg gttccacgac tggatttact gctcctatga tgacaatcgt
    54961 agcagaaacc gcaaaaaaag aagggtattc accagacttc atggcaactt ctgaagacgt
    55021 cgaagggtat ggtcgaccgt ttccttatat gatccataaa aacatgcagt acttcaaaaa
    55081 caagtcggtt gatgaagtaa tcaaagtcgg agatacggtc tcagatatcc aggaagggaa
    55141 aaatgccgga gtgttgacag taggtgtaat tgaaggtagt tcgctaatgg ggatttctta
    55201 tgatgagtac caaaaattag agatggggaa aaaagatcgt ttaaaacagt ctgttgctct
    55261 tagattccaa caagctggcg ctgatatttg tgtaaacaat ttaaaagagt taacagaata
    55321 cattttgtac gctgacaaga atccagaatg acttttatac aaaatcagag gtcgtgaaaa
    55381 agctgtactg catcaagtaa taagaacagc caaacaaaaa tagctcctca tatttttcgt
    55441 ttggttaggc ttattaccgc agatgcaagc ttttgaacac cgtttattcg aaaaaaaaag
    55501 ggagctgtga cacgactttt gtcacagtcc ccttatttac aaacaaacag tgtttaagag
    55561 acaacctctc cactaaacgg tgttcattca gctgactctc ggtatttgct caaaaaacga
    55621 taaaatttga aaagctattt tctcttttgt ttctctaata ctcggatcag aacgctgatt
    55681 tcacattctc tttttcttac atcttatctg gtgcatgaag tcctagcaaa cgaaggtctt
    55741 ctttcaataa tacagtcact gcataaacta gagctaagcg tgcttctttt tgttcatcat
    55801 cagctaaaat cttcgtatga gcatagtatt tgttgaatgc ttgtgctaat ttgattgcat
    55861 gtttcgcaat cactgacggt tcgtattttt ccccagcaga caagaccgtt tcagggtatt
    55921 tttgaactaa tttaaccact tcccagctgg tatcgtcatt caatgcatag tctgctgttt
    55981 ctgaagggac aaatcctgct ttttccaaca ggctgactgc acgagcatgt gtatattgca
    56041 cataaggtcc agtttctcct tcaaatcgaa caacttcttc caggttaaag tcaaatgtat
    56101 tcagtcgatc gtttttcaaa tcgtggaaaa tgactgcccc aaccccaact tgtttagcta
    56161 cagcatcttt gttttccaaa tcagggtttt tctcgctgat ttgttcttta gcagattgga
    56221 ttgcttcatt taatacttct tctaataaga cgatctttcc tttacgagtt gataattttt
    56281 taccaccttg cgtgatcaaa ccaaatggaa tatggcgcat atcgtcagac cagtcaaagc
    56341 ccatttcctt caagacagct ttcaactgtt taaaatgata gctttgttca ttccctacta
    56401 catatagaga ttgtttgaag tcgtaagtac gtttacggta caatgcagca gctaagtcac
    56461 gtgtaatgta tagtgtcgca ccgtctgact tcttaatcaa cgctggattc aggtcataag
    56521 cagataggtc aacgatctct gcacctttat cctcgttcag caaatgtttt tcttctaaaa
    56581 gtttgacgat ttcatccatc ttgtcattgt agaaagcttc accgttcaac gaatcgaaac
    56641 gtacttccag cagatcatag attttattga attctttcat cgattcatca cggaaccatt
    56701 gccataattg aatagcctct tcatcgcctt cttccagacg tttgaaccaa gcacgagctt
    56761 cttcatttaa ttctggttct gtttcagcta cttcatggaa ttggacatat aaacgaagca
    56821 attcattgat tggttctgct ttaacagctt cttctgtacc ccattttttg taagcgacga
    56881 tcagttttcc aaactgtgtt ccccagtcac ctaaatggtt gatgcggatt ggctgataac
    56941 cgattttttc cataatgaaa ccgatcgaat taccgataac tgtcgaacgc aaatgtccca
    57001 ttgagattgg tttggcaata ttaggagaag acatgtcgat tggtactgtt ccttgattcc
    57061 cgatattgct atccccataa tgttcttttt ctttcactac tgtttgcaaa acttttttgc
    57121 tgataagttc tttattcatg aaaaagttca agtaaggtcc gacaacttcg attttttcaa
    57181 agttggcaga atcgattttt tctgctaaat ctgcagcgat ttgctgtggc gcttttcggt
    57241 aaacttttgc taatgaaaag gctggaaatg caacatctcc gtgttcagct gattttggat
    57301 tttctagtaa ttgttcgact tgttccagtg ttaaatcatc tttgacagcg tcatagacag
    57361 cttttgcgac aagatcttta ttattcatct ttattcctcc taaagtgctc ccttgattgt
    57421 taaacaaaaa aaatctctag cgacggcata cttagtatgc catcactaga gacgagattt
    57481 cccgcggtac cactccaatt gtcctaaaaa aggaccagct ccatctgata tcggtagacg
    57541 accgtctgtt taaaacagaa ttctccaagt gcgcttcgtt gtatcttctt cattcggctt
    57601 ccaccacttc cgaattcgct agccaagaat gatacaagta ctctcttgtt caacaaatct
    57661 tagtcttatt aaactttctt gaattatatc agaaaaaaaa acgaaaggct agtcgtttct
    57721 gataaaaaaa caatccagat ggcctaaagg agaagaaata ccacctggat agctataaat
    57781 catttcgtaa atacgaaaga cttaacaaat taatcagcta cgataatcgt tccttcttca
    57841 gaagctaaaa gattctcgat attttctaaa gaagtgatga ttgccttagc gtttggtttt
    57901 gcttcaacaa atgcaatagc tgcctctact ttcggaagca tgcttcctgg agcaaattgc
    57961 ttttcatcaa tgtattgttt catttctgaa acagtaacag tttctaattt cttttgatct
    58021 ggtttttggt aattgacata cacattgtct actcctgtca atacgatcag caaatccgca
    58081 tcgatgattt cggctaattt ttctgaagca aaatctttat cgattacggc ttctcgtcct
    58141 tctaatccag tagctgtttc tacaacagga atcccaccgc caccaacaga gactgtgata
    58201 actccttgat cgaccagcgt ttcgatgaca cgtgcttctt taatcgagat gggtttaggg
    58261 gaagcgacta cttttctcca tccacgtcca gcatcttctt tgaatgtcac agtggagtca
    58321 gcattcattt gttctttggc ttcttcttcc gtgtagaatg gaccgactgg tttacttgga
    58381 tttttgaatg aaggatcatt ttcatccaca ataacttgag tgactaaaga aaccacatct
    58441 ttatcgatcc ctttttcctt cagtacttcg cccatggcat tttgtaacca gtaaccgata
    58501 gatccttctg tcattgcaac acatgtatct aaaggcattg ccggtgtttt ctcagaatca
    58561 gcagcttgct gctgaatcaa taggttaccg acttgcggac catttccgtg ggagatgatc
    58621 aattcatctc cttgttcgat aaatttcact aaatactttg ctgtttccat taacgcttct
    58681 tgttgggctt ttgcacttgc atccgtagac aagattgcat taccccctaa agcgacaact
    58741 acttttcgtt tggccatttc attcgtcctt tcttcagttc taccgaattt tctagataac
    58801 aaaacgagcc agcgttttgt gattttccct caatgctgac tcgttttttc gtgagatgaa
    58861 tgtggttatg cttaaacgcg agggataaac aagttaccta atgttgctgc catgatcgct
    58921 ttgattgagt gcatacgatt ttctgcttgg tcaaattgac gagcatattt gctgcggaag
    58981 acttcatctg ttacttccat ttcagtgatg ccaaagcgtt ctttcatttg ttcaccgtaa
    59041 acagtgtttg tatcatggaa agctggcaag cagtgcaaga agatcaaacg atctgtgttg
    59101 tgtgtttttt ctaccatttc catattgatt tggtaaggtt tcaacaattt gatacgttct
    59161 tcgaatttat cttcttcacc cattgatacc catacatcag aataaaggac gtctgctccg
    59221 tctacacctt tatcaacatc gtcggtaatc atcaattgtg caccggattt ttcagcaaag
    59281 ccttctgcca ttttgacgat ttcttcttca ggttgtaatt cttttggtgc aacgatacgc
    59341 atattcgctc ctaaaattgc acctgtgact aataaagagt tagccatatt gtttcgacca
    59401 tctccgcagt aagcaacagt gataccttct actgttccaa agttttcttg gatagttaaa
    59461 aagtcagcga tcatttgtgt tggatgccat tcatcagtca agccattcca aactggaaca
    59521 ccagagaatt ctgctagttc ctctaccatt ttttgactga atccacggaa ttcgattcca
    59581 tcaaacatac gacctagtac tttagctgta tcttctgtgg attctttttt acctaattgg
    59641 atatcatttg cacctagata ttcaggatgt gcacctagat cgatcgctgc agtagtaaat
    59701 gcagaacgag tacgtgtaga tgttttttca aataaaagcg caatattttt tccttctaga
    59761 tagtgatgtg ggataccacg ttttttcaag tctttcaagt gttctgaaaa atcgatcaaa
    59821 tattgtaatt cttcttttgt aaagtctttt tctgctaata agcttcttcc ttggaatact
    59881 gattctttca tattaaacat tctcctttag attgttttta tttaagatta aagaggaaat
    59941 cccgacggca gctgacagta agaccgccgt caggaaacaa attacttcaa gtcttcacgg
    60001 acaagtggtt ggctcatgca gcgtggacca ccacggcctc gtgacaattc acttgaattg
    60061 atttctaaca ctttgatgcc atagctacga agcaattcgt tcgatacata atttcggtta
    60121 taagtgacta caacacctgg agcgattgcc aatgtgtttg atccatcatt ccattgttct
    60181 cgaggtgcaa cgatctcatc tccattacca gttgggatca gtacaagatc atctaatcca
    60241 agagcagctt tcatcgtttc gtgtaagtta tctgaatgag taatcttgat atcatcacca
    60301 tctggttcga ttgtaaagac atccatctta ccgtctttac tttgaattgc tggatggata
    60361 gtgaatttat cgtgatctac cattgtaaat acggtatcca aatgcatcat tgcacggttg
    60421 tttggaattt tgattgctaa cactttttca aatcctgaat tttttgcgaa taggtggcgt
    60481 gctaattttt ctaaagcttt cgcattagtt cgttgagaaa tcccaacagc tactacttta
    60541 tcgcttaaga tcaattcgtc tccgccttcg atatgatctg gattttcacg atccaaccat
    60601 acttcgattc ctttatttgc aaatcttgga tgatgtttca aaatatattc tgtaaacatc
    60661 gattcacgac gacgtgcttc aaaagtcatc ttattgactg tcatcccatt tcccattgat
    60721 gcggaagggt cgcgtgtaaa gtacaagttt ggcattgggt ccatgtaaaa tggatagtca
    60781 tcgtctgctg acagatcgta tagactgcta gaacgtactt caatatcttt tgtacgaacc
    60841 ccagccatga ttttatctac catttcttgc gtttccattg atagaaggaa ctcatgaaga
    60901 ccctctctta cagcattcga tgcaatatga gattcatcca acattttatt caagaattgt
    60961 tctttgacat tgcctgcgtc aatcgcttct gccgctaatt tttccaagta aagagtctcg
    61021 acgccttcat tttgcaatgt ctttgcaaag ttgtcatgtt cttcttgagc aatcggaaga
    61081 tatggaatat catcgaacaa cagacgatcc ataatatctg gtgtcaaatt ttccacttct
    61141 tgtccaggcc ttttcagtaa aactgtcttt aatttcccta tttcagagaa aacgtgaata
    61201 ggtttatcca tgttgtgttg cctccttctt tttgattaca tgtataggat atcctccttt
    61261 tgtaatcact ttcataattt tgtctcattc aaaatgagac attcctcaca ttaatattcg
    61321 ataattaaat aattaaacgt ttttctattt ttaagcgtaa tttattcatt ttttattctg
    61381 tatacacttt caaaaaaata tttattcatt gaaaaaattt ttttgaataa attaaaaaaa
    61441 taaattatat tagcgttgtt agcgtttact atatgtatta ttgttgtttt ttatacacaa
    61501 atcattcgct tcaaaacaaa ttattcaata ccattgtata ttttttacat aataacaaat
    61561 tatatttgta aagtattaaa acttgtttct aatgttggta tgttaaaact ttacaaattc
    61621 tttaaaaaaa gtgacaataa atatagtgaa caataaggag aggacttttt aatgcgtgtt
    61681 aataaaatcg attttcactt ttcaaaactc agatatcaac ctgattttga acatttctcg
    61741 gatgaagagt tccaacagat caaggaacat actgttcttc gttcttataa aaaaggacag
    61801 atccttttcg acgatggtga tcccagacaa cggttgtata tcttgaccaa aggactcgtt
    61861 cgtttagaac gttatgatga aagtgcaact tattactact atgactatgt gaatacaaat
    61921 aacctttttc ctatgtcagg aatgtttgga gaagagacct acacttatac agctcaagcg
    61981 atgactgata ttgaaacatt ctaccttcct gtttcctact atgaaaaatt agcacgaaaa
    62041 aatcccgaac agttgatcta tatgatacaa aaaatttcta aagtagttga aatacatgaa
    62101 ttgcgtatcc aaatcggatt gacctctagc gcctttgacc gggtaaaaca aaatttattc
    62161 attttgaaag aagaattggg cgtaccaaca aaagatggtc atatcaccat tccttatccg
    62221 atcaccttaa aagaattagc agtgaatagc ggcacgacac gtgaaactgc tggacaagta
    62281 atcaaacaac tacgtgattc cggactgtta gattatgaca aaaagcgttt tactttctgt
    62341 aagacagcta ctactccagc tatgagagaa taacgcgaac aaaaataagc agtgaccctg
    62401 atatttatca gggtcactgc ttattttcct ccctaaaact tctgaaagat ccggcagttt
    62461 tagatcactg ggcaagactc aaagattttc tatgtgtgtt tcagttatgt aatattcact
    62521 gtttgcaaaa aatactgata gattaactag aggaagcgtc ttgtcctcat ccgacctaga
    62581 ttattcgata ttcctcgaca ataaacgatt aatcagtgag ggcaatcaga ggaacggata
    62641 atgatgaata agctgtaaaa aatagggctt tgacaaaagc gtgtcacagc ccttatttcg
    62701 attaaacggt gtgtacctgt caactcttcg gtcacaagtc ataatgttca taaacatgag
    62761 gagctgtttt ttgaaattgt tccttgtgaa tcagagttaa acgacaggct tacgaactct
    62821 tgattaaacg gcgttcaaaa gcttgcatac gcggtaataa gaacagccag gtcaaaaaca
    62881 tgagaagccg tttttcctgg gctgttctta ttacttgatg cagggcagat ttttcacaac
    62941 ctctttattc agataaaacc aagcttggat cagcattcaa ctgatagcta gcaaaatttc
    63001 ttttttgcat ttccacatgt gctgctgctc caatcattgc agcattatct ccacaaagtc
    63061 gaagaggggg aatcactagt tctacctcag ggagtttagt tgccaaagca tcctgcaatc
    63121 cttcacgcaa cccttgattg gccgctacac ctccagctac aatcagttgc ttgactggat
    63181 aattttgaca agctcgaagt gttttagtaa tcaatacatc gatcacactt gcttgaaagc
    63241 tagctgctaa atcatttttg tctagatctt ctccacgctg ttgtgcatta tgcaccaaat
    63301 tgataaatgc actttttaat ccgctaaaac tgaaatcata attatcttca tggatcatcg
    63361 ctcgtggaaa atggtaatta tcttttccct gatgagctag ctgatcgatt tcttttccgc
    63421 ttgggtaaga aagacctaat actcttccaa ctttgtcata agcttctcct gctgcgtcat
    63481 ctcttgtctc cccaatgatt tcatatgatc catcttcttg catatagact aattctgtat
    63541 gtcctccgct aacaagaagt gccatcaatg gaaactggaa tggtttaacc agacgggctg
    63601 catagatatg ccctgccata tggtttacag gaataagcgg caattggtgt gcccaagcaa
    63661 aagccttagc tgcagaaata ccaatcagca acgagccgac aagtcctggt ccataagtga
    63721 cagcgactgc cgataaatct tctgccgaca caccagcttc taccagtgca tcttccaagc
    63781 ataaggtgat ttgttctacg tgatggcggc tagctacttc aggaactacg ccaccaaaac
    63841 gtttatgact gtttatttga gaagcaacga tgttagataa gatctctgtt ccatttctaa
    63901 cgaccgctac actcgtttca tcacaacttg attcaatcgc cagaatcaat tcttcattca
    63961 tttcatcgtc tccgttttta actttctgct cataatgatc gcatcttccg tcggattgcg
    64021 ataatatgat ttccgtttac ctaatatacg aaacttttct gattcataaa gttttcttgc
    64081 tgcttcatta gatagacgta cttctaaaaa aacatgcagc gaatctttta tttgttccgt
    64141 aatcagtaat tgtaacaaat gccgtgcatg cccttttcct tgttctgaaa ctgcaacagc
    64201 aatatttgtg atctcgactt catctattac tttcgagtat ccaataaagc cacagatctg
    64261 gttatcttca actaaaagca gataatcaga gtgaggctgt tggatgtctg ccaaaaactg
    64321 cattttcgtc caaggtgaac catgtggata agcattttcg ctaatcatcc atacgcattc
    64381 tgccaagtac gcttcatcaa aatctttttt tctgcaaatc aactaatctc actcattttc
    64441 taaaagtttt atcatatcca acgcatcttc ttttgtttca gtgtaatatc tttttttgat
    64501 cgttgttgct tgaaaaccta acttccgata aagtctttgt gcatcttgat tactcatacg
    64561 cacttctaat gacatcgtct cacagcgatt cattatagca aagttctcga tttcgtcaat
    64621 aagcagcgaa ccgattcgtt tacgctgata tgccggtcga acggcaatat tcgtgatatg
    64681 gcaatctaaa ccgaacgctc ttgaaccgat aaaaccaatc attcttccat tatcaaagat
    64741 acctagatat agatgaggaa gcggtgactt caattctgat ataaaggcac tttttgtcca
    64801 tggcaattcg ccattataga cttctcgttc gacatccagc agctctttga tatcttctgt
    64861 agtcaattct cttagtacgt aacgacgatc gatttgttcg attgtttttt ctgtataaat
    64921 cattttatct tgaaaaaagc ttctggcata cgctctaaat tttttcaaca taactttcgt
    64981 ctccaggtgt gtgcgtctcc aaccattttt cttctgcttc tacccgtttt aaatagcgag
    65041 gaagaaaagc ttgtttctct gcaactggtt cagctaaagc acctaattga gccacagtta
    65101 caccacttgg aatatcccat gctggaactg tattaatcat cgcactaggc aatatttcct
    65161 ttatttcata gaagaatttg tgacaatcac tcccgacgaa ataaatagct tcattcagct
    65221 tttgtagttg tgtgaacaaa tcagaagcag aaatatgtac atcttcaagt acaatttcta
    65281 atctattttc tttccagcgg tatgccccag cgtatacatt ttttcttcga gcatcaaaca
    65341 tgggcacaat aattttatcc acaccgatgc aattagccgc gatggttttc agactagata
    65401 ctcctactag ctctttatct aatgtataag caatcgtttt tgctgtcgtt acacctatac
    65461 gaagccctgt ataagaaccg ggaccatcag aaaccgcgat tcggtcaatg tcttttggac
    65521 ttatttgaag atctgcaaac aattggtcga ttgcaggcat cagtgttaca ctgtggttgc
    65581 gtttgatatt tgtttgtatt tgtccaagaa tttgttcgtc ttctaccaca ccgatcgcca
    65641 aagtttggtt tgcagtatct attcctaatg taatcatata tttgatcctt tctgctatga
    65701 cattctcgcc ttccattgta gcatactttt ttattctgag gatgaaaata ccaaaggcaa
    65761 aactagtaca atgatacatg attatctgct ggcgggcaaa ttttgcccct tcctgaaaca
    65821 caaaaaagct gcggtatcac ttcatttccc cacagctttt ccatctttta tttcgttgat
    65881 tctcgaataa tcaactctgt ttccacaata atctttcttg aaaccggtgc tttggaatta
    65941 cttaattgaa gaatcgtctc cactgctagc tctcccatcc attcggtata aacttttaca
    66001 gtagaaagag agggcgttac atatttcgct acactgacat cattgaaacc catcacagaa
    66061 atatcttctg gtaccactaa acctttttct tgaatcgctt tcagtgcgcc aatcgctaat
    66121 gcatcattag ctgcaaaaag cgcatcagga acatgcggat tcgtttttaa atactgcttc
    66181 actgcttgat atcctgactc gacagaaaaa tctgcttcga taattggacc ttcgtcgtag
    66241 cctttttctg ccaaaacttg ttgaaatgcc ataattctgg gatcttttag ccgctgatgg
    66301 tttttcttcg tatattctgt tccagcaagc atagcgatat gttgcgcttt atgggagaca
    66361 aaatgttcaa tgactttttg aacacttgac ttaaagtcga caactagaga gtcgattcct
    66421 tgagaagtgc cgtcagaatc taccaaaagc aaggcatttt gggatccttt catttctaat
    66481 aactgagctt cgtcgaattt tcctagtgcg atcgttccat ctgtcggaag atcactaatg
    66541 gaatcccacg tttctttcaa taaagtaacg tttgcttctt ctgccttttt ttcgattcct
    66601 agacgaatag ataaataata aagatcttca agctcttccg tatcgtcgta ccactggatc
    66661 aggcgaagcg tttgcttagt atttgctgca tttttacgat gtttcgtata atttaagcgt
    66721 tctgctgctt caaaaatttt ttgctttgtg gctaagccaa cggaaagatc aggatcatag
    66781 ttgagaactc ttgaaacagt agcaggagaa actcctgcca actgcgcaat gtctttaatt
    66841 gttgccattt gtctctcctc cttttttacg tttataaaat tgcagtaaat cgttcaaacg
    66901 ctgcctgtcc tttttcatct cgcttgaata ctccagcgtc ttccagtaca cgtgcaaaaa
    66961 cctgacctac agatttttgt acgacatcgg tgaccgtatc tttcgtcaaa tcaggatgtt
    67021 ctttttttaa ttgttctgcc cacggacgat gatattcagc catctggttt tcttctccta
    67081 ataagaaatg ctctacttct actagttctg gtttcaaacg aggtggtaaa acagcaagcc
    67141 ccattacttc gatcaaccca atgttctcct ttttgatatg ttggacatct ttatgtggat
    67201 gaaaaatccc atcaggatgt tcttctgaaa cattgttgtc tcgaaggaca aggtccaact
    67261 caaagtaatt gtcccttctt cgtgcaattg gcgtaatcgt atggtgtggt gtaccatctg
    67321 ctgaaaaagc tctgatagat actgtttcat ccgaatatgc tttccacttt tccaatactg
    67381 agttagctgc ttcaatcaac aattttttct ctgttgattg caaacggatc acagacatcg
    67441 gccatttcac gactcctgcg atgacttgcg gataatctgc tagtttgata agtttttcta
    67501 tcggtgcttt tgccatagga aattcatgac gtcctgcctg ataatgatca tgagaaagaa
    67561 tcgatcctcc aacaatcggc aagtcagcat tcgaaccaac aaagtaatga ggcaagactt
    67621 cagtaatccg aagcagtcta gcaaatgttt cctttgtgat tttcatcggg cggtgttgtt
    67681 ctgataaaat gatcgaatgt tcattgtaat aagcataagg agaatattgg aatccccagc
    67741 tttcaccgtc tagattcatt cgaataatcc ggtgattggt gcgggctgga tagttcaagc
    67801 gacctctata tccttcgttt tccatgcaaa gcatgcaggt aggatagtct acttttaccg
    67861 cctcgcgttc tgctgcgatt tgttttggat ctttttctgg cttagataaa ttgattgtga
    67921 tttccagttc accatactcc gactctgccg gaaatttgat gttttttgcg atggctcttg
    67981 ttttgatgta gtcatttttc ttacttaaat taaagaaata ctcagttgct tcctctgggc
    68041 ttttcgaata gtgctgtgca aagaatgcat tgacgactga aggtggcggt gtgagaaaat
    68101 ccattaattg cgcttcaaaa atctcttgtt ctgctggcaa ttctgaaaga acaccatttt
    68161 ctcttgcctt agcgacaagc tgatccaata agtctacaga ttcttcctta actggtctta
    68221 cttcaatgct ttctagagca tcttctccta ccatccctaa aagacgattc tgtaaatata
    68281 agcggtccat ttccatccat ccgccagctt cgatcgcaag tgtggcaaaa tcacaaatcg
    68341 tttgactgat tgacatatct cattccccta ttcttcataa ccatttggat ggctgacatg
    68401 ccaatcccat gctgttttga taattgcttt gatatctgtg tattcaggtt tccaacccag
    68461 aacctctctc gctttttcac ttgaagcgac taatcggcta ggatctccag ctcttcttgg
    68521 tgctatttct gctggaattt cttttcctgt cacttctcta gctgcttcta gcatttcctt
    68581 gactgagtat cctttgctgc tgcctaaatt gaagaagttg ctttcatttc cttctttcaa
    68641 gtattccaaa gctaaaatat gtgcagcaat caaatcttct acttgaacat aatctctgat
    68701 acatgttcca tctggtgtat cataatcgtc tccataaaca gccaatgctt ttctttgacc
    68761 aagagctact tgtaaaatga ttgggacaag atgcgtttct ggtgtatgat cttctccgat
    68821 agatgcatct gcttttgctc cggctacatt gaagtagcgc aaagcgacat agcgcatgcc
    68881 atatgcttgg tcacaccact tcatcatttt ttccatcatc aatttacttt caccatacgg
    68941 atttttagga ttagctggtg tatcttctgt gattggagac tcttttggtt ctccgtaagt
    69001 tgctgcagta gaagagaaca cgattttatt cacattgaac tcgtgcatga cttcaagtaa
    69061 gatttgcata ccgtatacgt tgttgttgaa atacatcaat ggtttttcga cagattctcc
    69121 tactaaagag cttgcagcaa aatggattac tccttcaatc ggttcctttt caaatacgga
    69181 acgtagaaat tctttatccc ggatgtctcc ttcataaaaa tgagcatctg gatgtactgc
    69241 ttgtttgtga cctgtgagca aattatctac cacagctact tgatacccct tttgaactag
    69301 ctggtctaca gcgtgcgaac cgatataacc ggctccgcct agtactaaaa ttgtcataaa
    69361 aaacactcct ttgattagta aaattgtgtt tgttgattat atataattta aaaaaacgaa
    69421 gtttactttt atggtgaaaa caaacaaagt aaaatttcgt aaattcatat ggtaaattta
    69481 taataaaaca tttagtaaac attttcaagg attttttgca aaggtttaca aacaaacgta
    69541 aaactagcct ttttttctct tttgttccat aatagatata acaaataata aatgaggtgg
    69601 attcaatgaa aaaatttgta tctggaatat tagtaggaag tctggcaaca gccgctgccg
    69661 ttgctggttt ggtcgcttct gtgaaaaaaa cagtgatcga tccaattgac gaaaaagaag
    69721 caatgatcga agaaaaccgt aaaaaagcaa tgcgcaaacg cgtttctcgt taataattat
    69781 ttaaaaacac gcatgaagac ttgtgaaaga acagataagt catcatgcgt atttttattt
    69841 gtcagtcaat ataagttgtg ctattttatc tgcctgtctc agattccatg gatctgtata
    69901 aggtctagct actgctgcag ttagccaatc cacaccttca acaggtattc catagcaata
    69961 aaagtcagac tggatttccc ctgaagcatc aatcacgcga ttcgtacctc gctcaacatc
    70021 tatagcttct gaatgataac ttcctgtatt atctgaaaat gaaaaagggc gaacaagttt
    70081 gttttccttc aaatttttta tgacaggatt acttgaacga gaaaagcttg tttgtggcaa
    70141 tcgagcttca atcaaatagc gactgctgat ttctttatct tgtagagaaa taacatagcg
    70201 gttgtgcttt tgctgaatat caaatggcgg ttcgaacaaa tgaaaaatac ccgcatctat
    70261 cagtgctgct aattctttcg tccgaatgac tggcggaccg atcgttaaaa agctatttag
    70321 atgagtaaac catccccata gtatttcttt gtattccttt gcagaaaaaa cgtgccattc
    70381 aatagcctgc cggattggat cacgccagtc ttttaatgca tcaaatgttg aagcaaatgc
    70441 tcctgtgcaa ttgccttttt ccgcttcttt tatctgccct tcaataaatg ctcggctagc
    70501 ttctgcaaag gactcatcct gataggtaag aggaatttct aaatacgacc aatcccatct
    70561 ataagggatt agtgctggat gctttccgag acactcctct gaagagaggg agagaaaatc
    70621 atgctcaaaa tttttctttg gaatcggcaa atgatattct tcgatgattt tcttgtaata
    70681 aaacaactca acgtcttttt tcagatagtc aaaaaatgtt tcgccagtca aaaatcccga
    70741 ctgatgccat tgctccaggt tttcttttgt tagtaaccgt ggccatgcca ttgatccgcc
    70801 ttgcttttgg tttcttcctc gagggtaata agggagcccc cttcttgatc cactgtatac
    70861 aatcggttcc ttgcctgatg gatgatagac aagttttcct ttttcgttct cgaagcgccc
    70921 ttctcttgat actgtaaaca atccgacata atcaaaaaaa cttaagccta gacctcttaa
    70981 aaagacattt tctttaggcg gtatcttcga aagatcgaca tctgcaggat tagaaggaag
    71041 ctgatacagt aaatcatttt tctttgcgta gtctacccac actctttctt cttctgtctc
    71101 atcattttcc cagtgaccgc ttgctaaaac aagctgatcg atagaatagc tttgctgatc
    71161 tgtctgtaaa agataaccat cttttctttt gctgactttt tttactaaag aataaacaaa
    71221 ctgaatagaa gctggaaatt cttcttgcaa ctttgagaaa aaccagcgtt gatagagtcc
    71281 gtataaacat cgagtagatt gctgatcttt ttccagacga ttgatttctt ctataattag
    71341 ttgcttattt ttggttaccg tctgttcgat ataatctgta gcaggtcctt tactccattg
    71401 aaacaagttg ggtccctgtg ttaccaatcc tccactggac aatgtttcat ctgtaaaaag
    71461 cgtcacttgt tgagaaacag aattcattaa taactccggt ggctgattca accgccaaac
    71521 tcgacctcca ggaccattgg gatcgaaaag aaaaagttca agagaatgtc tgtcctgatt
    71581 attatggaga atcctttctg ctatacttaa tcctctaggt ccggcaccaa tgatccctat
    71641 tttcatttgc atgcctccat ttcgataaat tttttcaata aaacagcatg gttctgatgt
    71701 tcattttttg caccataaac aaaagtaatg actggatgtt ctttcataag ttgactgagt
    71761 ttaaaaatag cttgcttctt ttcttcttcg aaaatcaatt cttgctggta tttctttgca
    71821 aatgaagcaa atcgttctgg tacatggtca aaagttttgc gtgcttcatt actaggtgca
    71881 atttctttca accacagatc taactgcgct ttttcttttg aaattcctcg tggccagaac
    71941 ctatccacga gtattctata gccatcctct ttttcagcat caaggtaaac acgttttaat
    72001 ttgatcatag ttgtttcctt ctttctgcct tttcgcttag cttatatatg tagataatag
    72061 cacaaaataa aaaaaatgaa cggcacaact atccattgcg ccgttcatgt gatcttggtc
    72121 aaaatctaat gattgattcg gtttttaaag ctgtcataca atcatctgtt ttatactttt
    72181 ttacttttga tttttccaga ccatccgtca tatccaccct taagtaaata aatctcttga
    72241 taacctgctt tacgtaattt atttgctgta cggatgctca agcttttctt ttgatcgtaa
    72301 atatagacag gctggtcttt tcgaatagat cctaatgaat ctttcaaaac agtgtaaggg
    72361 atgtttcttg cacctaaaat atgcccagca tcaaacacat ctttttcgcg aacatcgatt
    72421 acttgtgctt ttctcattcc ttctcgaaat tcttcttctg tcaaagtcgt tgctgaacgc
    72481 ttggccatta ctcgtagata gacttcccaa aagatgattg caagcacaat caatagcaaa
    72541 atgatattaa ttacccataa aaccatgtaa tgcctccgtt tttttaagca aatttcaagg
    72601 ctaatgaggc agcaccaatg acaccagctt cattaccaag ctctgctaat ttgatttttg
    72661 ttgattctct tacttgcggg aaagtaaatt ctttaaaata tttctctaca cggctacgta
    72721 aaaattctcc tgctgccgat actcctccac ctaacacaat acttgacgga ttcaatgtat
    72781 tccccaagtt ggcgcaagct aaacctaagt agaagcatac gcgatcaaca accatcaatg
    72841 caaacggatc gtcttcttga gccatttcaa agatatcttt acttgttact tcttcaccgt
    72901 tgtctaacat tgctttcagt tttgaatctc ctgcgtattc ttctgccaag taacgaccta
    72961 gacgtaccac accagtagcg ctagacacag tttctaaaca tccgcgttta ccgcaagtac
    73021 attcgaaccc ttcaggatcc acagtgatat ggccgatctc tccgcctgct ccgctcacgc
    73081 cgtgaatcaa atttccgcca gcaacgatac caccgccaac acctgttcct agagtaacga
    73141 atacaacgtc tggatcgttt tctccagcac ctttccatcg ttcacctaaa gcagcaacat
    73201 tcgcatcatt atcgatagcg aatttgattc ctgttcctgc ctcgatcagt tttttcacaa
    73261 attgaacttc tgtccagttt aagttatacg caccaataac cgttccagct tcgctgtcca
    73321 cgcttcctgg ggtacccatg ccgattccga taaagtcttc ggctttcata ttatataaat
    73381 tcaaacgatg gttgattgat tcaatgattt caggaacgat atgcttacca tcatccaaaa
    73441 tattggtatc gatgctccat ttttgttgaa tctctccttc tggtgttaaa attgcaaatt
    73501 ttgctgttgt tccacctaaa tcaatcccaa taatcttctt gtccattaca aatttccttt
    73561 ctttcggctt tcttcaattc ggtgctctct ttttaatatg cgccacgctg tcatgtatgt
    73621 atcatgatca atcaaacggc tatcatacag attacgcaat tcgatagtca ttaattcaat
    73681 gtcatagatg cgtgcaccca catagataaa gatgccaaat tttttgaaca gctgctgtac
    73741 atcatataag ctctccatct ttaagcactt ccttcatatt atacaagtaa accatatttt
    73801 tttaaaccca gtaaaataca tattacaaaa agaaatacaa aaactagtgt agaaagaatc
    73861 ctttggtgta tcgaatagct tgtctgttgt ttcggtagac ccaaaacatt ccctaacact
    73921 aatccgccaa ttagcccacc gacatgtccc caaacatcta cagaggtatc cagaagacca
    73981 aaaacaaatg tcaacacaat aaataaaaga aaatgtctga ctagctgctg gatcgctggg
    74041 ttatgtttaa aatgaaaccc taagataaac aacgcaccga acaaaccgaa tatcgaagta
    74101 ctcgcaccac cagacagaac attcgcttcg ttaaaagcaa agcttgctgt atttcccatt
    74161 atcccgctga acatataaat caagaaaaaa cgccaatggc catagattgc ctcgatttgc
    74221 tgccccataa aatacagtat cagtgagttc attacgaaat gcatcagtcc aaaatggatg
    74281 aagattggtg tcactaaacg ccaccattca ttgaagtgaa caagataagg accatacatt
    74341 ccggcataat aagggatctc aagtcgcgga aataattcca tcatgaggaa gaccgctgtc
    74401 tgtatcccta aaaaagcata ggtccataac ggtcgtctta accatgcacg caattttatt
    74461 tgctgttgat aattcatgaa aacctcccat ttcattttag tcaataaaca attgttggat
    74521 cttctgatca aagctctccg gttgccattg ttcattcaat tgttcacgaa aaacaaggct
    74581 acatgttttt ttatgaaaat tatttaaata ccggtcgtaa taaccaccgc caaaaccgat
    74641 tcgataaccg gctctattgt agataatacc tggtacaatg aggagatcga tcatctccgg
    74701 caaatagagc ttctctgatt gtggctcaag aacaccaaaa ctcgaacgta caagaacgga
    74761 ctgttcatcc atttcataaa acgccatctg acgtttcggc aaagttttag gtacaactat
    74821 tttcttttgt tctttttctg ctcgttggat aaccggctgt gtatccagtt caaattcatt
    74881 cgataaagtc gtccctatca cttttgctga cttccagtac tcgctggcga atagctgttt
    74941 atagattcgc tgttcttttt cctttttctt gccacttttg gccagatttt tcagcttttc
    75001 gattcctttc cggcgtaatc ttactttcaa ttggtccaaa aactcaccct tccttctctc
    75061 ataaaatggt ataggaaaaa aagaaaaata gcaatcgaac tttcaaataa atgcataaat
    75121 agaataaaaa gaagacctag aaatgattgg ccttcctttt ttaataaatt tcaactgttc
    75181 agcgcgtgtt tttgtctgat aaaaagtgca tacagaaaat atggcgcacc aaaaagggca
    75241 atcaaaattc ctgtaggtac ttccccactt ggtagaatca ctctagcaac catatctgca
    75301 ctaagcagaa aaatggaacc gaacaaaccg ctcagaacaa aggtccagtt attttcttta
    75361 cgtacgacta gcttcgcagc atgaggagct agtagtccta caaaagataa actacctgtc
    75421 accgctacac taccgcttgc caaagcaaca gctaacaaca aaaggatttt ttgactttta
    75481 gccacgttaa ggcctaatcc ttgcgcgcgt tcttgaccaa atgatagcaa tccaatcctc
    75541 tgtgcataaa gtcctcctag cggaattaag ataaccgccc atggaagcaa tgcccaaaca
    75601 taaggccagc tcgcacccca gattgatccc gctaaccatg cattcacaaa tccataattt
    75661 tccttagata attggatcgt gcatagcaat gttaatgcag aaaggccggc attaacggca
    75721 ataccggaca ataagattcg attcataccc aaaaagccat ttcgatcata ggccgcaaga
    75781 tagacacagc aagcagctaa aacaccacct agaaaggcta caactagcaa tagaaacggc
    75841 agcgaatagt gcgaaaagaa acctaaataa agcatcacta aaaatcctgc tccggcatta
    75901 atgcccaata tactagaatc tgccaattca tttcttgtta ctccttgcaa gatataacca
    75961 gaaacgccaa gacaagctcc tgctaataaa ctgatcagta atcgaggaac tcgaaattct
    76021 ctaacaatca gcgccaaaga agatgattct ttccctatga acgtggcaaa aagctgatcc
    76081 aggtaaataa aaggcgtacc aatcattaaa ctacctatag acaggatgac aagtacaaaa
    76141 agcaagaaat agaacgtcca atatattttt ttcaccgttg ttccctcctt acttgtatca
    76201 gtaaaaacgg aacgccaatc aatgcaatga ctaatccaaa aggtgtttca aaagggggat
    76261 tgatcatcct cgctgtaaaa tccgccaaca taaccaatcc accgccaact aaaaaagaga
    76321 caggtagaac ataacgatag tccgttccaa ccattccgcg aacaactggc ggaaccaaca
    76381 gcccaataaa tgaaacaggt ccgactaacg aaacggcaat ggctgctaac actgcaacta
    76441 gacagaaaac aatcaaccgc gttcgtttag gatgacttcc aagagctaat gcatgtgttt
    76501 cactcaaatg aatcaaagat attgcttttc ctaatgaaaa agaaaccaaa acagcaagta
    76561 tatataaagg aaacaatact gctaattgtt tccatgtgat attggcagct ccgcctacaa
    76621 accaaaaagc aagatcctgc tgcagattaa agaattgggt aagtgcttgg ctgatagcag
    76681 taaaaaaaga actaatagct acgccagcta aaatcagctg taaaggattc atgcccattt
    76741 tcgaaaactg caaaaaacca aatacgagca aagttacagc aaaagaacct gccaatgaca
    76801 ttagaaacac agaaaaatca gaagctttag gccaaaaagc aaaactaagg gccattgcca
    76861 acgaagcacc cgcgttgatt cctagtaagc ctgaatctgc taaaggattt cttgtcactc
    76921 cttgcatcaa tgcaccgctc aatgcaaaac aactccctac aagaaatgct cccgccacac
    76981 gaggcaaacg aatatttcgt atgatttggt gactttgttt tgatctatca aaatcaaaca
    77041 acgcctgcca aaaatctgct tttcccacag tcgctgcgcc atttgaaaga gcaacaaaaa
    77101 aaataatcaa aagaacaaaa cagaccaaaa gcaggagtct atttttcttc atagtcctca
    77161 taattgatcc tcctgctctt ttggcaacaa atcataggtt agaaaaactg gtttatttgt
    77221 tcttggaagc gtgacaatcg ttgcatcaat ctcaaatatt tttttcatac actcttttgt
    77281 gatcacttct tcaggtgtac cttctgtgat caacttaccg tttttcattg ctaataaata
    77341 atctgaaaaa cgtgaagcat gattaatgtc atgaatcgac atcaagatcg ttgtttgttt
    77401 catttgattg atttcagtca ataactgtag gatctctaat tggtgtgccg gatctaaaaa
    77461 tgttgtcggt tcatccaaga tcaagatatc tgtttcctga gcaagcgcca tagcgatcca
    77521 gacacgctgg gtttgtccac cagacagcgt tgcgatatcc cgcatacgaa atccagctag
    77581 acctgtagca tccaacgccc actcaattgc ttcatgatct tgcgtgtcca tgccactgaa
    77641 cagtttttga taagggtatc gtccgtaaga tactacttct tctactgtca tacctgtagg
    77701 gtgttcacta gtttgtgcta ataatgcaat tttttttgca agttcttttg aactgtatgt
    77761 agagatatca gagttattga caatgatctt tcctgatttc acaggtagta tacgggtaaa
    77821 acttttcaat aaagtagatt ttccacttcc atttggccca atcaaactag tgattttccc
    77881 ttcaggaatc gtaacagaca tgttttcaat gatttttttg ttttcgtagc cggctgcaat
    77941 gtcagctgct cttaatctcg tcatttcacc catcctttcg ctgaaaatca ttctcaatta
    78001 tgacgctgtt tcaacatttc gtcaagcgct actttcatat ctatagccaa atgaagcggt
    78061 tgacagcgtt cgaaatatca ggaataataa ccaatgagaa agattctcaa ttatgttagg
    78121 aggaaagaaa tgaaattaaa aacaaaagta ttcacattat ctagcatatt gctgctggct
    78181 ggagtactga gcgcttgctc cccctcttca gataaagaaa cagctacaac tgccaattcg
    78241 acgacttcat ccaatagtac tcatacagtc acagatacac ttggacataa agtagagatt
    78301 ccaaacgcgc ccaaacggat catcggcagt tacttagaag actatctgat tgcactaggg
    78361 gaaaaaccta tcgcccagtg gactgttggc agtggttcca ttcaagatta tctgcaagat
    78421 gatttaaagg atgtcccaac gatttcttat gaccttcctt atgagaaagt gttaagcttt
    78481 gagcctgatt tgctgttgat ttcttcttcc gctactgtcg aaggcgggaa atacgaacaa
    78541 tatagcaaaa tcgcgccaac ttatgttgta aaaaacggaa cagatgtcac atgggaagaa
    78601 caactaaaag atgtcggcaa agctttggca aaagaaaaag aggcagaaaa aatcatttcc
    78661 gattatcaaa aatccatcaa gaaaactcga gaagaactag cagataaaat cgagaacaag
    78721 acagctgcag tgttgtgggt gaccaacaac tcagccttta tggtcagcaa aaatcggtca
    78781 agtggaagaa tcgtatacga cgatctggaa tttggtgttc ctgatctagt ggaagaggtc
    78841 tccaaaaaag ccacttctga ctggtctgct gtttcctctg aaaagctagc tgaattagat
    78901 gctgactaca tcatccttgt gaacagtgac aaagatgctg ccatgttcaa tgaaccaagc
    78961 tggaagaatc taaaagcagt gacagacaat catgtactgc aattcggacc tgaatctagt
    79021 tggctatata acggacctat tgccagtcag gaaatggtca atgatatcaa agaaaaacta
    79081 aattgatttt aactaaaaaa agcatcttca cgattagtcg tgagatgctt tttttcttat
    79141 taacaaacta gacgcataga ttttattctt cttttgaagc aaccctattt tttactcttt
    79201 cccatttttg aaaacgatgg gcatatagat tttcttgatc cgttatcccc tcatactcct
    79261 ctataagggc gaagtctgaa aaatcaatat tcccaatata cgtatcgcct tcaaacgtgc
    79321 tatcgatcaa tgttcgccag atccgaccag tgtaaggaag aagttcttga aaaatccgac
    79381 taccacccga aacatagata tcttctgaga tcgtttgact atacgacaaa agttcatcga
    79441 tcgaatgcat tacttccgca ttttctttcg tgcgaaaatt tggttgcgtt gtcaacacga
    79501 tgatatggcg ataaggaagt gaaagatccc ccattccttc ataggtcttt cttcccatca
    79561 ctaaaattcg atccattgtg tgttcgcgaa agaaacgcat atcatttggc aatcgccatg
    79621 gtaacaaacc atttttgccg atcagaccat ttttatcttg tgcccacatg gaaataaaca
    79681 tattatccgc tccttatgat tttagatgtt ttgagataaa aaaagtctca attctttgac
    79741 cagcaaggaa ttgagaactt taaaaacatt attttgtttc acggtgcaaa gtaactttgc
    79801 gttcgcgtgg gcaatatttt tgtttttcca aacgatcagg attgttacgt ttgtttttgc
    79861 ttgttagata gttacgttct ttacaagaag tacactctaa agtgatgttt acgcgcatgt
    79921 ttttccctcc catttttaca agaatcctct aagatgactc tcagacttga ttatcttacc
    79981 atgatttttt ctagattgct agtgtttttc acgattttgt taagtaaaac tacttgacga
    80041 taaatacaac ggttttactt ctcaaacaaa atctttttct ttttgtataa atacccttac
    80101 acttgtttat atagattatg ttttttaata aagggggata ctaatatgcc atggaacatg
    80161 aaggactttc ctgcatcaat gaaaaactta gataaattga caagaaaaaa agcaatcgac
    80221 atcgcaaatg cattattaga tgaagggtac cctgataacc gtgcgatccc aattgccatt
    80281 gatcaagcaa aaaaatggta tgaagatgct agtgaatcag aaaaacgaac tttcgaaaaa
    80341 gaaaaaaatc caagcaagac ggatgaacac gatacgaatc ctcgtgctgg aaaactgctt
    80401 gattcagatg tgatcgtaga atatgaggaa gaacaatgga tcgtgaaatc taaaggggca
    80461 aaaaaggcaa gcaaccattt tgatacaaaa aaagaagcaa tcgaaaaagg aaagcaagta
    80521 gcacaaaaca aagaatcaac tttgttcatt tataaaaaag atgggacaaa agaaaaagaa
    80581 atcgattatt gaacatatgt aaaggggttg tgacgaaaat cgtgtcacaa cccccttatg
    80641 ttcgaaacaa tggtgttcct ctgccaactc cgaaccataa gtcataatat ctataaaacg
    80701 ggaagagctg ttttttgaaa ttgtttctta tcactcagtg ttaaacggta ggagaaaacc
    80761 tcttttttat tgatatttga ttttcatcaa acagtcacat agtcgattgc ttctgcttgc
    80821 ggaatcgttt catctacaaa gtagacttta taccatgtca agaaactgaa gattgtccag
    80881 atttttcttt gctcttcttt acgtccttcg tgatggtcat ctagcaactt catgattttt
    80941 tcttgatcaa aaaattcttt tgcaaaatct gcttcgaata atgctttgac ttgttcgtag
    81001 ccgtgttctt ctttcaacca agctttgatt ggcacaggga atcctaattt cacacgattc
    81061 gaccattctt ccggaagatg tttgttcgca gcttgtctga aaatatcttt tgtgttgttt
    81121 tgattcaata aaaacttact tggtatttgt tgagccaatt tcatgacttc gatatccaat
    81181 aaaggtactc ggacttccaa agaacttgcc atagaaagtt tatctgcctt taacaagata
    81241 tcttttggca tccattgatg aagatcgaca tattgcatct tattgacttc atctttgatt
    81301 ccttctgttt tctcgtaatg aacagtcatg atttcatcca cagatggtgc tgtttgatat
    81361 tttggtgtta acacttctga tgcttctgat tcttcgaaca ctcgagcatg accgataaaa
    81421 tattcttttg caggagcaag tgattcatat aaatggatcc gtccatggaa atttttcatt
    81481 tttccgattt tacgtcctaa attatagcgt gttccttttg gtaatttttt caatccttga
    81541 gcaatcacac ggatgaattt tgaattcgta tggaatccat acgtttggta tcccgcaaac
    81601 agttcatccg ccccttctcc agattggact acccgcacac tttgtgaagc aagatttgct
    81661 aaaaagtaca aaggtacgca agaaggattt gaatcaggtt catctaagtg atactggatc
    81721 aaagggaatg ctttgaacga catgtctccg tcgatcactg ctgctgtatt atctaagtcc
    81781 aatagttcgg ttagttttct ggcttcaatc gcttcattat aggttttatc atcaaacccg
    81841 attgagaaag aatgatctgg tttcaataca gaggttacat aactggaatc aaccccgctg
    81901 gataagaaag aaccaacttc tacatcactg accgtatgtg cttcaataga agcttgaacg
    81961 gtttcgtcga ttttttcgat ccattcttgt cgggaatgcg tttcttttgt ttcaaaatct
    82021 gcatcccaat attgctgaat gtctattttt ccatctttat acgtataata atggccttca
    82081 ggcaaacgat agacatcttt gaagaatgtt tcgccgttta atggagaata ttggaaagtc
    82141 atataaggtt tcaacgcttc tttgttcaat tctttgttaa aatcaggatg aggcagaaaa
    82201 cttttgattt ctgaaccaaa cataaatgtt ccgttcattt ctgcataata atatggtttg
    82261 atcccaaagt ggtctcttgc accgaataac tcattctttt cattatccca aatagcaaaa
    82321 gcaaacattc cgcggatctt ttgcaataat tctgttcccc attcttcgta accatgcaaa
    82381 aggacttctg tatctgcatg tgtttgaaaa acgtggccag ctgcaatcaa ttcttctctc
    82441 aaaggctggt aattatagat ttcgccatta aaaataatga tctttgtccg atcttcattg
    82501 taaataggct gagtaccgcc ctctaagtca ataatactta atcttctgaa tcctaaagca
    82561 acatgtttat cgatatattc tcctgaacta ttaggtccac ggtgaacgat tcgatccatc
    82621 attgtattaa taattgtttt tttgttgtcc ttgtcgttaa caaaacctac aataccgcac
    82681 atttaacttt cacatcctaa tataaaatta ctgtttattt cattcatatg catcttatca
    82741 tagaataaat agcggacgaa tattttctct cttacaattt cgtcacgtga aaacaaaaga
    82801 tgaaagtttc tgtttttaca gaaaataaag tcccaatatc taatttacca tgttttcttt
    82861 tttgaggcta ccttattctc ttcgtgttaa aaaaatatta gtggatgcta tttccaaaaa
    82921 aaccagcaaa aaaagacacg acctaaagat cgtgtctaat tatgactgta tcagaaaata
    82981 tgataccaaa cggatctatt cattaattaa ttgacatagt ttatctgttg ctcttttatg
    83041 actggataac tgaccaattc atgacgatcc aagtcttctt tattcatatt tacagcagta
    83101 atttgactgt cttcatacgt acgataataa taaatccctt tatcaacgtt acagcaagaa
    83161 gaataaatcg tatattcgta ttttctttct ccagtatcgc atactccttt ttgttgctcg
    83221 actgagccta gtatatgaaa aaattgactg atactttcag attctgaatc acctgatgca
    83281 gcattcattt tagtaaatgt ggcttttaca aatcgagaaa cagaagagag atctccaggt
    83341 aagcccaatc ctcccatccc acgactataa acattcaagc tgatctggtt tgaaaaatga
    83401 ttttctggtg ttccatttga tagcgagcga taattgttca gattaaataa ttgataatca
    83461 aatggcggat tatttgtcaa cacaccgaca ggattatcat ataaatgaag tccatctttc
    83521 atactttcaa ttactatcga tttttcttta tcagctaata accagtgtaa aggtgacaag
    83581 ggtagttttt cactaaaatc gatattgact agattgatag tagccaataa ttctttagct
    83641 tcgtctatgg ttgagcattg ccctagtatc catggaataa actcaaatgg tgcaacgtta
    83701 tcttttcctt cttgtaattc tttataatca gcattgccag gaaaattcag tcctgccata
    83761 ctcaaccctt tttcatttgt agcctcataa tagagtggat agttggatac cccagctgct
    83821 attcctatca ttgcataatg tttatctaaa ttttttacct ttctaaaatc gaaacgataa
    83881 tttcttggag taatagtgac cacttcatta taagacatct cataatcaaa attccttcca
    83941 aaataatggt ctttcgttgc ataagtgatt gcggtacaca tcgtttcccg tcctaaaaca
    84001 aatcgttttt acacttttat cttacatcta gtattttttt tatcaaaaaa aggccaaaga
    84061 agattgattt actagcattt ataaaaaata gtactcaggg ggaatcaaaa aatattgtag
    84121 gttaaaagta caacaataag gggctgtgac aaaagtcttg tcacagcccc ttattccgaa
    84181 caaacggtgt tcaaaagctt gcatctgcgg taataagccc aatcaaacga aaaaatagga
    84241 aaagctattt ttgtttggcc gttcttatta cttgatgcag gacagcttct tcacaacctc
    84301 taatcgttga tataaggaag gtacaggatt tgaacctgcg cgccggtatt agccggttcg
    84361 ccggatttcg agaatagggt ttaaggctga ttttaagtgt ttttcgctta ttatcagctt
    84421 tttattatct aaaaaaacaa cttttagagg catccatata ctgccagggg gagtcaaaag
    84481 gggagtcatt ttttatagtt tattcgcatt taatcgacgc tgtaattctt tcactgcatt
    84541 agatacagga ctgattacac cgtcttgtgt tgtgcctaaa tatctttgta atgcttttat
    84601 tgtattttgg ccacacaatc cgtcttgagc aatgcctaag aaaccttgta aagctctaat
    84661 gacattcgac ccagttaatg tattgtcaaa ttgagcagcg taaatatttt gattaaatgt
    84721 ttgtttgaac tgatgactga tcacaccatc tttgccagct gtgtcaaagt attcttgtaa
    84781 acgtctagct gttgcattgc caaactgacc atcaagagct aattggatca tctgtggttt
    84841 attgtctgta ttagcagttc ctccatcgag aatacggtag aaattatgtt gcagttttgt
    84901 actcatgtat gcatcattag tatctactgc gatcccgtta tgcgtataag agcaatggat
    84961 aaatgagctg ttacttagaa aaatacctgt atgcccagct gatccatttg aaccaccagg
    85021 agtacctgaa ataaagatat cccctctttg tacttctgat cgactaatct ttttcagttt
    85081 tgttccaacc ataccgaaca acgtttcagt atttcccatt gatccagaag gtagaaagcc
    85141 tccagcaatc attgagaaga aaactgcaga gctgcaatcg taagaattag gtccaagccg
    85201 atgggtcatt gaatacgtca ctttacctag tcgatcctgc atccatttaa tcattgactc
    85261 tttactcatt tttattgtcc tccttctttt ttgtttttcc cttgtttttc agttgaatct
    85321 tttagcttgc tgaatcgatc tttcacaaaa tctggaatcg ggatccctag ctgacctaaa
    85381 ttctcaatca ccgagattcc ataaacagca atgtacgaaa gcacaaaagc tgttgcgact
    85441 ccttcaaagt tcatgatttt aagatagggg taagcaatca gtacaagcgc cacgaccagc
    85501 aaatgtttta ctacccccat tagtccttta gtactatttg tttcttttcc agcaagtccc
    85561 ttacaaactc ctgtaatgat atccgcaatg acaatccaaa caaaaatttg aatataaacg
    85621 ttgcttacta gatttttaaa ctctgataac aaaactaaat tatcaatgat caccatgttt
    85681 ccacccttca ctttctattt ttggccatac aaaaagcgca ctcgttcgag tacgctatag
    85741 tgactaactc acttgtggat gtttacaatt gctcgttttg aaaccttatt accattaaat
    85801 tttgttacaa gataaattct tgtaattttt gaattttctt ctgtttctaa taaccaactt
    85861 ggtttgaatg ctaacacttt tatcccctcc tttcttcttc gatagtaata tagcacaaca
    85921 cgtctaaaat acgatgcgta taaagaaaaa gtttatttat attaactatg ttgttatcac
    85981 aacactttag ttttattaat aattttttgt gcttcttcgt ccgtaataca taatggaacg
    86041 aattgtcgaa cttggtcagc agtaaaacaa ttccagtcat acatcatctt gatatcatca
    86101 taagaataca tattattttc ctccgtcaag ttgttctttg atagtagcaa tgtcttttgt
    86161 gttttgaagg gatgtcagca tggttttcga attaatttgt gctagtgatt ctgcttttgt
    86221 agtaagctct tcatttgctt ttttcaatgc agcattatct acctgtaaac ctatagataa
    86281 attttctagt agttccaatt ttttagaata atcttgtgta actgcttctt cccacttatt
    86341 ttcagtgaag ttaaaaaatt gcgactgttg tcttcttccg agaccttcat ccgtttcatc
    86401 ttccttcttt tcaaacaaaa taggtggaac ttcgacaaat ggtaaagatg ttggaaagtt
    86461 atcttctact tgatgttctt catatcccat tggatacaat actttataaa ttgttttcat
    86521 tttactcttc ctccaattag taatctgtta agccggccaa ggatcttgag tgatccacat
    86581 acctgaaata tatgaactac ctgaacctga tttggcttgt atgatgctac tttgatcaat
    86641 gaatgcacgt gcatccgctg gctgacttgc atttctaact agtgaaagcg cggtttgcgc
    86701 tggatagcct tgatcacgtt tgaaaccgtc cgggattttt aaaatccaac gagtttctga
    86761 tccttcgggc catgtgccac atttaaaatt aagagtaaga aatacgatgt ttccgattct
    86821 gattattttt ccattcacat cagttacgtt tgttgtatca cttcgatcag ctagtgtgat
    86881 agctcgctcg atcatacctg cttgcacagg caatccattg aattgcaatc cgtctttaaa
    86941 atttttcgtt cctaagaccg tttcattacc tgtggctttt actaaaacac cctccacacc
    87001 atcaattcct ttggcatgag ttttcaaata cttagcaact ccgtcttctt ttaattgcac
    87061 aatatcagcc attaaaccgt ccccactttc tcaaatgtaa tattggctaa tccatcgagt
    87121 ttcactttat cttctttgga catcaaacca tttgctgtcg tcgtagccac agctgtcgtt
    87181 gttgcattgg ctccaggatc gcctttgtcc cctttatcac cttttggcaa tacaaaatta
    87241 aactttgctg cacttgcagt accagcattt gtgacactag ctgttgtacc actcgttact
    87301 gtccctacag taattgtggc agcctgtcca gggtctcctt tatctccctt gatagtttcg
    87361 ggtttaccct caacagcatc ccaatgggtt tgagggtaaa cctgaacatc attctgtttt
    87421 acttttacaa tatctgtcat ttgtctatac ctcccctact ttttcaaacg atacatttgg
    87481 tattttggaa tctgtataag cttgtgcatt ttgataagct tcactcattt tttgatcaac
    87541 atattcttga gagacgccgc caccttctcc accacctgtt gcagagatta ctcctgtgtt
    87601 tgaaatactg atattagctc cggcagtata gttgaattgc ttatcggccg ataaaacatc
    87661 atcagtcatc gttaaacctt caccgacacg aataccacct tttacaaatt cggaggcagt
    87721 gggtaaaacg taaaaaccag tcacttctgc atttattttt ccatcttcat caatagtgat
    87781 gttttctcct gcagtatagt ttttcagtga atcgagtttt tcttttaact ccagcgaaaa
    87841 attgacatca gattgtctga ctgcagagac ttttcctgtc tcatcaatcg tcaataattc
    87901 accaattttt atgccaccca aacggtcgat agtggcaact ggcaacacgt agtcaccttc
    87961 tccactgttt aaaatttttt gatacatttc tgacgtgata atgccgtcat cttcttcact
    88021 tgcatagggt aattctgtga gaacattctc taatcccaaa tcttctttag taatgacgac
    88081 ggctccaatt ttgccattaa cagataaaac tgttgcttct cctgctatta tttcttctaa
    88141 cccaagaact gcggaagcat gtgtgattgg aaagaattgg cgttgtacac ctgttttttc
    88201 atccgtttcc atcattcgtt tagttttagc cattagatca ccccaacttt ctcaagagtg
    88261 aacacatttt gctttggatc atcaacggtt gcgatgatca aggctccttc ttcaatcggg
    88321 aattctacag ttccaacttt ttcgacttcg tgattatctg aaaagagatc atcctgcaaa
    88381 atatcagtca cttcaatctc accgtattca atcgtgaaaa gtgttgctcg taatttctga
    88441 tatagataat ccatatctgc cagcaagcgc tctgaaatag acgcatgacg aactccttga
    88501 atatcaacac gtgcatccat taattcggct aacattactc cacctggatc gatagacttc
    88561 aaaatatctt tgattgattc aaaccatttt aaataatcgg tttcttgtcc ttctctccat
    88621 gcctcaaatg tattttgttg gtcttgtcgc cattgttcaa attccttttt acgtgcattc
    88681 atccaatctg tgaagtcacc tttgttttca ttgataaaag ctgacatatc cgcaattaaa
    88741 tcttcgactg tctgccaata cgatcccatt tcaccctctg tttttgagac agctttaatc
    88801 acaaaataag agaaatcttg cgtcgtagca attaactcat catctttata aatgataaaa
    88861 tctgctgttt ggcgatgtag gcattgcatc gaatattgat caaatgtgta ctgaattttt
    88921 cctttttttg catcaatgac tttagtacct aattgaactg ggtatttgtc tccgacaact
    88981 gattcaaaat agactttaca accagttaga tcatagggca ttccattttc agtgatggtt
    89041 gcttccataa cttctgaatt cttattacct tgccgtacct gaatcatacc gacatagtta
    89101 taaggttcag ttgtgctgag tactacgttc cactttgcca ttaattacca ctcctttctc
    89161 aaaatcaggt gggatacaaa tagaagctat ctgaccagat ccgaagaatt cacgatcata
    89221 ttccgccaca attccacctg attctgcatt ttgttcatag gtttgaatac gcccattttc
    89281 tagacctttg attacacctg tgtggccgta atattcatgg cttttaaatg tatcactgat
    89341 ttttgcgttt ctttcccaat tgataattga gccaacaact agttgctcat actcgggatg
    89401 aacaataacc gtccataaat acaatgccca tgggtaggct cgtccaattt cagcagcaga
    89461 aaatacattg ccgtgacgta ctttgatctc gtattgtgta cctgccccca tgtcaggacc
    89521 aatcatcaca ccggcatatt ccgcagataa tgcataacat tgatggtttc cgataggttg
    89581 attcaacaaa cttttcaaat gatttaatcc tttttcttcg atcacaaaat cacctctatt
    89641 tcttcgctct ggcgagacaa acatcgccgc catttaataa atacccgaag gaccaatcga
    89701 cgcgaccgat accaacacca ttggaattca tgccacccat ttcaacaata gaagtgctat
    89761 atcccttcca atcctctgct aaaatagcgg tatgtccatc attaccagca ccagcaccta
    89821 aattaacaat gatgatatct ccggctttcg cttcatttgg agatatttca gtcagatact
    89881 gacgtgcccc tcttgcatct gcggccattg aacctgtgta ccacagagtc ccacgtgttg
    89941 cggttttata tcctgctttt gtcaaagcca accacacaaa agaagagcag tcagcatacc
    90001 cattacgatc aggattctcg acgctaccaa aattccaacg taacggttgc gagtaatgga
    90061 aataacccat caagcttttg gctgtactga gaatactgcc agcagatggg acttgaagat
    90121 ctttgaattt gttataccaa ttaactgccc atccttgtct ttctggatga gttgtggctg
    90181 gtctttcata gtttcgttca aatgtataag cagcagctgc tggatcagtc attgctttaa
    90241 atccagaaac acttgccggt tgaacaattc ctaaccattg accattatac attgtccaat
    90301 ctaataactt tccttgagct gacattgttc gataatcttc tgtaattcct gcagctctca
    90361 tcaggttttg aacataagtc ctgccatccc atgttgctgg aggaaccaaa ggatatgctg
    90421 aaccatccca ttgaacgatt ccataggctg gaccgccaac ttgtgcaata tctggattca
    90481 ttgcagcacc ggcttctcct tgaacatttc caaggattcc agcaatggat gctttggaat
    90541 aacctctaga aagaagcata acccatagct cccaagcaaa tttgtccgct tgactggtaa
    90601 cctctggtgg gtacatgcca ttccaacttc ccccgccgtt attcccactt cccccttgtc
    90661 caggaaatac ttcttgccca ttaattgtaa gttttccgcg tatattgaca tttttcataa
    90721 actccacatc gccaaaagca ttcagctttt cttgcaaatt tagacttccg tacaatatag
    90781 aagtaccgtt tccaagcata actaagcctt ttccttcttt tggtgaaatg agaatatatt
    90841 ttccatcgcc gtttgttcga atgactaacg aattatcttc taacggtgta ggagtggatg
    90901 cgccaggaaa aggattgcct gcagaatcag tcgtaccaat tgtgccgata gattccttgg
    90961 tgttccagaa ctccattcct ttcttggtta gttccatgat ttttttatta tcattccaaa
    91021 tttgtaacgt acctttgaca atttttagca catcacctag atcattaatt gagctttcaa
    91081 gaacttctgc attaataatc ccaactttaa tgaaatcagc tacgatttca cccttcgacg
    91141 tcattgcgat accgaatggg ccatttactc cgttgtctga ataacctaag ccatttaaat
    91201 tccaacgcca cacacgcttc gcattcgcta cattgggagt atccataata agaatctctg
    91261 atggagcttt ttctggacga aaaacgacat gcccaccaga attaccagtg atccatgccg
    91321 tcgcattcaa cacattttga actaacgttt ctgttcgatt gtcgattttc tttttcaatt
    91381 cttgtgcttg agtatttact gccgaagtgt acagtgataa atcattacct aaaacaatat
    91441 ccttatattt cccaagcgtt gggaaataag tgtattcgac catgcgctct tttatctcga
    91501 tgtctagctc tttcgcccta acatgagcga catcaccaaa gtgtaaagac gctagctctt
    91561 gatacatctc accgtattcg agtgtgtgtt ccaacgccac catgctgact gtatgagtag
    91621 cttttggctc gtgaatacgg tcattgtcaa ataaggtttt gccccatttg atcaattcat
    91681 caactgtttt acaatcacca ttttcacgtt tggcaatacg tcgattctca ttggtcactc
    91741 catcaatttc taaataacca tattcgatag gttctttgtc ttcatcataa tcgttatcag
    91801 gaaccccgcc aaccaaatac aaactattga caattgattc ttcatcaatt tcttcttcga
    91861 ttgcttctaa attgatgcca aaatcgatcc gaaaaccatt atctgatcca atctgtttta
    91921 ccagtttcaa atcatagtta tccatatcaa gttccgcgct agtcacgcca gtcaaatttt
    91981 gattaccgtt atttgagccg ataatcgctt caactggtgc gacttgtctt gctgtaaatt
    92041 ggtgcgttgt acctacattc gacagatagt tgaatttttg atcaaatgcc aaactgtttt
    92101 taagattggt catgatttga gaaccgttgc cgttatccgt gaatgactta acgataaagt
    92161 ttttattggc cataaaacca atatgtctgg ctgtcacaga aacggatgtc aaattctttt
    92221 tgatattgta aatttcgaag tattgccagg aaccatctgg taccttcgct tttataaaat
    92281 ttcctttttt taaataagag cgatattgtc cgcttcttga atagttacca taaaaacgat
    92341 attgaccatt taatacacga ttgatttctg gcaaatcttc ccagtcaacc aacgacgctc
    92401 cgttaacact caaatcatct ggcatttttt tgtaagcata aataaattct tgtgtcacag
    92461 atacacactc ctattccaaa accgcacttc tttaaatttt ccggatattt ttacctgatt
    92521 ccattcaggc tgcaatactg gccaatcacc acgcgtaaat aaattcaaac cttcttgtat
    92581 tgctttccca agctgagtat caaccacgat tgttgcagct aatgtattaa gtattgttag
    92641 gcttttatta ccgacggtga tcgtaatatc tcctccgttt gattctattt ccaaataggg
    92701 atgtgcaatt tcgtcaccat gatcgaaaat atccatcaca ctagaatgaa aaattttagg
    92761 cgcttcaccg atttttcttt tgagtggctg acaacgaaaa gtaacatcga atgtataaaa
    92821 gaagccccac tcgttttcga aaggaacttc tttatccatg ctgcagatag cgtctagata
    92881 cttgtctgga tcattatgcg tgattaattg acttttacca gtaagccatc gtttgacttc
    92941 cctcagtttg gaatgtggga tagtgatcct ttctatttcc aaatcaaaag gttcataatc
    93001 atcaaacgtc tccgtcaatt ctccgcttcg gccctgaatt gtgtaagttt cgtatcgctt
    93061 gttcggcata atatctggaa gctctccctc aataatgcaa tccatatcaa ttactgcatt
    93121 ccgatttttc caaataaaat tgggttcatc tggtttcata aattgtcgaa tcaaatagga
    93181 acacccccca aatcataaat agcctgttgg tttgctttag caaatttacg attcatacga
    93241 tctaactcag atggattgtt tgcatcgact ttgccaatgt ggatatgctg ttcaatatta
    93301 ccaccagaaa cttttccgcc aatacctctg cgtttttctt cgtcagataa tggggtaact
    93361 gttgtttttc cattttttgc agtcagtaac tctggaccag cttcaccaac aatcgcttgt
    93421 ccattaatta gatggccccc ttgtgctaaa taaggcagtt ttgaaataga aaatgatttt
    93481 ccacctacac caggaaccca ttttggtatc tttatattat tcaatccacc aataaatcca
    93541 ttgatcaatc caatcatggc attaatagga gctttcccta cagcaacaat accatcgaaa
    93601 atacctccaa aaatatcaac tagaccttgc catgcttttg accaattccc tgtaaatact
    93661 ccagtgataa aatctagaaa accactgaaa attcgttttc cagcattata aaaattattg
    93721 aaatttgcta taacaccatc gaaaaatcca ccaaaataac cacttaaaaa attaaatatt
    93781 tcaacagcta catctgaaac acctttgaag aaagaattca ctccatcatg aaaccattta
    93841 acgttattat atgcccaaat tagtccagta actaatgcgc caatagctat aactgctaat
    93901 gcaaaccagc ctcccgacat tccaaataaa ccagcaagtc cttgccaaac tcctataaaa
    93961 cttttaactc ccccaacgat ttttgtaaca gatcccataa gtgaaccaag aacgattagt
    94021 actggtccaa ctgctgccgc tataccagca attgttatga tccaattctt tgtatcttga
    94081 tctagagacc cccaccattt agagaattcc tgtaataagg aagttgcttt ttcaaaaaaa
    94141 ggcaaaaggc ttacttggac tgcttcacct acgtcagcca ttgctaattt tgcattgttc
    94201 atagcttgat cggcttgatc aattgggtcg agagtagcat cgaatgtgtc tcctacagct
    94261 cccccacttt ctccagcagt ttttgctaaa tcttccaggt tcaaagtacc tctacgaatt
    94321 gcatcggcca ttctaggacc gcctttagtc ccgaatactt ctgctgctgc attgatggct
    94381 tctgtttcag aacctgcatt ttttaccttg tcttgcaatt ccccgagacc ctgacttaga
    94441 gattttccat cttttgcata agctactgtc gctttagata aactactcaa agctgcactt
    94501 gagtcaacac cgctttgttc aaattgtcca agcaaagtga ctccttctcc gaagctcaat
    94561 cctagttgtt taatttgagg tgcgccatca attgcttttt tcatcaagtc atctacagat
    94621 tgaccagtgt tttgcgatgt ttttgttgtt acatcgagaa ccgagtttaa atcttcgtac
    94681 tctagtccat aagcatcaat ggcttgtcga gcagatattg ctgattgcga aacatctgta
    94741 tcattgattt cagcgtactg taggagataa ttggttgaat cttccagttt tttatccatg
    94801 aatccaaatt gtgtatttac ctcaccaata gcttcaccaa cagtttgcaa tggtaaatga
    94861 gtattcgaac caacattttt gaaggattga gaaagcctat ctgcttgctc gcctgtcgct
    94921 cctgttttag tgatgatagt atcgagagct tcgtctactt caccaaatgc ggcaagacca
    94981 gctgcacctg ctgctacaat cggcgccgtc acaccgacag acattttttc accaacgcct
    95041 tttatctttt ccccggtttc ttctattttt tgaagctttt tcgctgtatc tacagaaacg
    95101 tcaccttgtt ctttaagtgc atcgttcgtc tgttctaatg ctgtacgaag tttattttct
    95161 ccggtttcag aattgagtaa ttgtttataa agtttttctg attcagcaga atattcgcct
    95221 gtctctttga cagatttttc atattcttct ctcaacattt gagatcgttg ttcagctaac
    95281 cccagttgtt tttctaactt ttgtttagcc gctcgcaatt tttctgtttg agttgcatcc
    95341 ttgtccatcg cagacacttg gtttttgtac tcggcagcgg ctaagttcat ttctttgttg
    95401 atttctttga ttgttttcga ataactgact tcgccattcg ttttaaaatt aagaacgaca
    95461 tcagattctt taccagccat ttatcttagc gctcctttct accaccaagg cgatttatcc
    95521 atagttacag attgaggtgg ttcaaaatcg gtgttttgtt gtaaccactg taaataagat
    95581 ttgagccaca agttaggtgt agacttcaaa aagaaactct cactccaatt caatagagtg
    95641 agagcaacgt aaatataaaa actccaagga gttcctatct cttccgattc tttttgttta
    95701 cctttctttt tgcttgcgtt ttttgaaagt cttgtggctt cttggatttt tttaggtctt
    95761 ccacctgaaa tgtctggcta gcaaaaattt ccatgcaagt actataagca gacaacacct
    95821 ctccactcat tcccaaaaat ttgaaaatag tttctggatc ttcatctaaa ccaccagtac
    95881 gcagcatggc ataaattaag gcgcgcatga tttttaggtc actaggcgat aagttagcag
    95941 aagaaatttg tccttctttt ttagatagca tggcgttcat atcagattca aattgcgaat
    96001 aatctccacc atacacatct gctataaatt ccatcgtttg catagtaaat gaaataggga
    96061 attctgcgcc ttgaatagtg acaacagcag aattctttaa atcatcaaca tgaatcccat
    96121 aatcggcaag tctggccatt atttgctacc tccagtttct ggtggtgtag tttctgctat
    96181 caacgttttc cattgtgctt cgtcgtagac tggttgtgca atgaattttt caaataaatc
    96241 cattgtcgct tcttcacgat tcgaatcaaa acttgcatac atcacattat tgtatttcag
    96301 tcctgtagaa acaaagtttg cagttacatc atcgattttt gtttcatctt cagcagtagc
    96361 gtattcttca tcaattacat tagatagttg cgtgtttgga taccatacag cttttttacc
    96421 tccgccttca atatttccaa tgaatccaaa tgcaaggtaa ggaaattctc gtgcggtatt
    96481 tttactaaat gttactccgc tttttgcaat catccctttg atttcatcca ttaccgcaat
    96541 tggaatacct acatgatcca acgcaatttc atgttttgtt tctcgggaca cgcgtcggaa
    96601 cattttgctt gatgcccatt tttccaacgc tgtaccattg cctttgactc caatttttgt
    96661 agcgataggc aaccggacaa cttcactata agtcggagca gttccaacag aatctggcgt
    96721 tgccatcatc gcgattaaaa tgtcgtccaa tccttcaaaa taaaaagtat cttgctttcc
    96781 caattaaaat catccttccc ataaatcaag tattttcttc gtcatgatct cttcgatttt
    96841 ttctttattt tgttcatacg taccgcttgc aaagtgttgt gctttttgtt ttgtcgtacc
    96901 attttcggta aagcgccagt aaaaagcagt ctcttcaaac tggactttca ctcggtcttc
    96961 ttcaacaaca acttttattt gttcgctcat atgcttcttt ttaagcaggg acttaggaat
    97021 attaggaagc aactgctcta ggtaaaactt tgcagcttct tctaaagatt ccaatgataa
    97081 tttttttgga tcaactctag aaagattccc taaataatcc gatatttcag aaaaaccatt
    97141 cttattactt ggcattttcc atacacctca catatgtgta atagttggtt acggtatcgt
    97201 cgttctcatc accttgtata cctgtaaaat ctgaatatgg aataccagca ttttgtaacg
    97261 cttcttcaat aacagctaaa tcctgttctg ttccaagtgt gaaaaaagaa atttgataat
    97321 acggtaattt tttatagact ttaccggatg ccattttttt actgttactc acatttgagt
    97381 acacgatgta tggatacatc gtccctaatc tggctttatc tctgaacact ggcaactttg
    97441 ttgatttcag cgctgttttc aattcatcaa agctaatcga cataagctaa actcaactcc
    97501 atttctcttg tatcaggatt cgtataaatg cgagtaatgt tatacgttac agaatcaatt
    97561 ttgagcgcac ttaatttctc tgtgatggat ttatcccatc tgactttaat tcgtctgaca
    97621 acgtctgtct tggcttgctg tgataaatat ttttcttgag aagtcacacc gagttcttca
    97681 tagaatatta gacgcttgga ttcataaacc gtagttggac gatcgttctc atctgttcct
    97741 gttttgattt ctagcaattc agctttccat cgcagattat tggtctgtcg tttcggcatt
    97801 ttgaatcact ccttgtacaa taaatggcgt aatagcgctc aaagctttgt ctaactcatc
    97861 ttcagatact cggtattcgt aggcaatgcc ggcaaccatc aaaataagat attcttgttg
    97921 gccaccagtt gctgttttga cataatcttt tgccatattt aaataaaaag agagcaaaga
    97981 atcatccatg ccctcttcaa aatgaatatg tgatttgaat ttttcctcta aagacaattc
    98041 tttagtttgc tcttccatct taaccaccaa ctggttttgt aatttcgtag cgatatactg
    98101 ccggttcaaa tggagaataa accaattgac catctagcaa gttgtaaatt tggaatccaa
    98161 tttgattttt accagagaat ttttcaacaa gtttttgaat ttccaaggca ccaataactt
    98221 cttgaatttt aaatgcagaa aaatcgccaa aatataaaac tggtgtgtct ggtttaccct
    98281 ttttatctgc tgcatctgtc caatccacag gatagccaac taattggtaa cctattccac
    98341 cttctgcttg tgtaaatgga cgcaacaaag gaaatccatc atctgttttc attttttcaa
    98401 tagcagtcaa agcagctcga ttaataataa agcgtccctt tttcatcact tctgtcactg
    98461 gtgtattttt aaattcgatt aatgcatcat ataatttttg cccagcacct gcagcagtta
    98521 gatctaaagg tttttcaaat gctacagcct tcttggctaa tgcaccagga ttttcatttc
    98581 cagcgtcatc accattgaac atataattga tttctttacg cacataagct tttttcaatt
    98641 cttccacaac aatatcttca actggaacac cagacatttt tagtaatttt ttagttactg
    98701 ttgccaaagc atcgaattcg gcaggatcaa gcaaaatttc atcaaactga atagctgttt
    98761 cagcaatatc agttgaacgc tctttcttgt ttacattcgc atctgctttc ttcacaagaa
    98821 ttggatattt gacatctcct gatgttcgca ccactgttcc gtatttacga agtaaatttt
    98881 cttcttgagc ataagtaata acttcagatg caattacttc tgggacagta actgaaccgt
    98941 tgccagcttc aatccctaaa gctcgagctt ctgcttcaga aatatttcca actacaaaat
    99001 tagcaaatgc ttttcgtagt tgttgatctt tttgtttatt ggtcattttt gcacgtgcct
    99061 ccaatccgtt cttaattgat ccaagtaatc catctcgttg ctgttgagta atcattccag
    99121 aacgattttc tgaactatct tcactgtctg aatcttcttc ttgatcgttg tcttcaccag
    99181 aacggctttc gtctgaatcc gtactattcg attgatcatc tgtattgtcg tctgtttcat
    99241 cagttccaga atctgcgcct aattcgtctt taattccgtt caattcatca ataacactgt
    99301 caatttcttc tttcacggct tctaaatcag cttcgcgtaa ttctcctgat tcaacttttt
    99361 cacgtaattc agtcaatcgt tgttcacgac gtgccttcat ttttttcaat aattctgtat
    99421 ccatgtattt ttcctcctac gcttctagcg cttgattaat ttttttgatt aatttttttc
    99481 tttcttcgac gtttttttct aattcatcac gacttcttaa tgctgcctct gtatcttcat
    99541 aagccggcaa aggaacaata gaaacctcat ataattccac ttcgctaatt gttcgaagca
    99601 taggttctga attgtaatcc catgtttctt cagttggtac aaatccaaaa ctacattgat
    99661 taatgtctcc gcgttccata gatttaacca agtctcttgc taccgttgtg tctggcaaat
    99721 cgacttcgaa tttcaaacca tgatcgtctt ctgacaatcg aagggttccg cttttggttc
    99781 gccctagtac attagaccaa tcgtggttaa ataagcaacg tacatcggag ttactaatcg
    99841 ttctagcaaa agcgcctggt gcaatgactt cactcaaatc gtcccataat agtgttgggc
    99901 tattgaatac agcagcatat ccactaatgg tcctcgtatg agtttcttca tcagaacgcg
    99961 ttgaaaggtt ggtgatgtca atcgtgcgaa tttccttctt cttcatttcc atcacctcct
   100021 ttcaagtttt gatcattcgt tggtaaggaa tcatctgttg catttttctg gccaatccta
   100081 gacaagtcat ttgaaatata gatagcttgt gtttctggag tgttctgttt aggaaaacca
   100141 agtatttctg ccacattatc tggacttgta atcccagtac gaacaatgtt gtagccaata
   100201 tttgtttttg ttgagtaagg aacaaaatcc aaaatgttaa ttttccattc cactcgatag
   100261 ccagaattag gcataaaaaa aagagcggtg taatgttcgc tcttgttctt caatattggt
   100321 ttgattgctt tgttgtgcag atacatcatc gctttttcga tgtctgtttt catcaacgat
   100381 tgatacgtat caacattgat tcctaaaaat ttacctaagt cttttttata aacacctaaa
   100441 tagttcaaaa tagccgcgtc atcaacagga ctttttaacg tctcgatgga atatcctttt
   100501 cccagaggaa tcatcttaac agaatgatca ctgtcatttt gcgttccttc cagttgatcc
   100561 aatatagctt tgacaatttt cgtttgggcg ctattatttg gattgatgtg ggcgtccagt
   100621 ttaagcatga acgcgagtaa accaccttta gtatatttgt ccgtcaaaac tttttcagcg
   100681 ctcagaacgc cttccagagt gtttcttgca aggtcaatta ttccagcacc ttttaatgaa
   100741 tcagttccga tgttcttaat atgtcgaatc atttgaccag gtattttttg tccattcatt
   100801 tcaaattctt cttgaagtcg ttcattgatt ttagtggtta caccgtatgc caaatgaagc
   100861 tggtcccgat ctgttaatgg gaatgtctca ccattgatca gtagggtatt tgtttccaat
   100921 ttggtaaatt cgaatccggt caaataatca ttgggattct tcaaaatttt tagcaagtgg
   100981 tggtccttca cttcattacc gtctggacct atgacaacag gtgaggacaa cgctacctgg
   101041 tttgagatat cctggaccaa ttcataaaca tcagaagatt ccatgataga ggaatctgtt
   101101 acatatcttt gaccgtaacg cgtatagtgg ccaaacatat cctcgatgta cccacgcttt
   101161 tccataaagg aatagactgc attcgataac cgatcacgta atttcaatat ttctcaccgc
   101221 ctttctattt atctatagat ggaactaagg taatcatcta attcatccga atcaatatct
   101281 gtcatctgat tcatcgtttc cttatgacca cacaaaaacg ccacgaaacc atcaatcttt
   101341 ttctttgatt gacgtttact tggcgctttt tgtccgttaa tgttagtgat tgctacaacg
   101401 ttcaaggtgc aataaaggaa caatggatta tcaaattgaa ttcgtttctc ataaaacaac
   101461 cgttcgacat catcaaaagg agcgttcaac actttaggat attgggcaac ctcaacgcat
   101521 tccaatccta agttctccaa tttctcaaca agtttgtcgc tcatcgctgg atcataattc
   101581 acttgttgta tatcgtataa atccatgcag tcttcgatgt actgcaagat ttgatcttga
   101641 tcaatcattt taccatcgca aaattcaaca aaaccttgtt cagctaaatc gctgtaaggc
   101701 acgttatctt ctttttctcg aaactctaaa tcttcattgg gaataaaata aagctgcttc
   101761 actttaagga ccgcttttcc atcttcatcc cacgttggga agtttaaaga cacacaggtc
   101821 aaatctcgtg tgcgtgataa atccaaacca atgtaacaag gttcaccact taaatttcca
   101881 agctcttggg tagtaaccaa acacggctct acttgatcct gttcaaagaa attatccgca
   101941 ccgtttacaa atacatccaa gtgcttcgtt agaaactcgg cttttgaatg tgctgaacgt
   102001 tgtgccgttt taaacgctga ctccaaggcg gaaagatcaa ctgatattcc ccagttagga
   102061 ttgcacattt cccaaacttt tctatccgtc caatcatagt ttttatttgg ttcatagatt
   102121 agaacaaagt tcgaatcatt atcatcacgt ttcaacactt cttttgcttc acgatatacg
   102181 cgcattccaa cagacgatga acctttccca gcagttgaaa tattaaacat caatggttgt
   102241 ggcaaagaga tttgtgcaga cttaaaattg tcatactgtt ccattttctc ttgcttatgc
   102301 agctcatcat tcaatacaaa atatggatta gaaccctcaa tgttatcgat attcttcgtt
   102361 tgaacaataa atttattcga ataagccata tcttcatgaa tataatcata agtaatacta
   102421 gaaacagttc cctttggtcc tttgaatatt ttagttcctt caagtaggat tggattgttt
   102481 aatattgtag ctgcaaaagg tttggcagca tattgagctt gagcaaagtc agaagcgcat
   102541 gcataacaat cgactgacaa agcaccttct ccatacattg catagcctaa tgaaccgaca
   102601 gcaatcaatg ttttaccgtt ctttttaggg atttggacat acgcttcacg agtaacacgg
   102661 acaatttgcc ctttttcatt ttctttaacc catccataca tccacgagta aataaatttt
   102721 tcccaaggtt ctaaaatgaa tggctttcca accatctcac cttttgtgtg aacaataaac
   102781 gactcaaccc agtccatcat ttcattcgca cgatctacat caaaccaaat atctttacgt
   102841 tttttccacc gataccaacg atccactgcc aaacgaacag ttttaggata tttcccaggt
   102901 ttctttctta cttcttttgc aaataaatcg gcataattta caccaggttc gatcattttt
   102961 cagtacctgc cttcttacgc catttgtttc tgtgcgctgc tagttcatct actggctttt
   103021 cttctggacg tgtaatttct tcatcttttc ttgcagtcga tccgccagtt atttgtctac
   103081 cagttttagc cttattcgtt agccccaaca aatctagagc tttcattttc ttatctgacc
   103141 aagtttctac ttgctgcgcc aatggatgct tcgattgatt agtggcacct gatttattcg
   103201 tgaatttttg cgtctccgga aagccttttt ccttccacaa aagatatttg tgttggtaaa
   103261 tttcaaaaat atccaaatat gattcgatta atggatcaag agtgatggtg tacaaatcag
   103321 acaaattcat tatttttaaa atacgagctt tttcagcact tactttttca tcaacaatcg
   103381 ctttacgttg cgctttagtc gtcatacttg tatacacccc cctttgtttt ttgaaaaatt
   103441 tgacctaacg atgcgcgtga ctccccgcta ccctatctcc ccacgcgaaa aaattttgaa
   103501 aatggatagg gggggcttca attgaaatac gaagggaaaa cttttttgtc ttccgtttca
   103561 ttttcaacaa ttggatgaca ttttgaacat aaaagcatga gattgtttgg atcaagctta
   103621 agcagttcat tgtctttgat tggtacaatg tgatggacgt gtgccctctt gccaaagatg
   103681 aactgaccac atcgctgaca gtggccacct tctctttcgt aaataaattg gcgcatatct
   103741 ttccatgctt gcgttcgata gaatggtttg ttctcatgat gataaacaga ctttgcttgc
   103801 tgcttcttct tgcgtgatct gcttgatctc ttgtgttcag tacagtagat accctttgct
   103861 atcttgttcg tgcatccgtc aaactgacag tatttcattc tgcttcacga ataagattta
   103921 tgatatcgcc ttttgcacgg atagcaccag gaatatcaat accatgtttc ttagcatatg
   103981 cacgcaattc ttttgcagtc atattgtcca gttcatccgt atcttcagtg gactgatcat
   104041 tagtagcttc gtctccatca aaatcagcag cagtgtttcc atcgctatca agaatttcac
   104101 tgctattagt aataagttca ccattaacag ccacaaacgt tttaccatca cttaatattc
   104161 ctttttcttc cgttacttca aaatcaggtt cttgaccttt cggaactacg acagtctttt
   104221 tcttttctga atcccaatac tctgttcctg tgatggatgt tctaattttg attattgcca
   104281 ttttgattct ctcctttgta atttgtactg attacttttg cgcccatccg ctcataccat
   104341 tcaacctgtt cttttaaatt cggtaaggtc ctagaaatca atgctatggt taggtgagtt
   104401 ttgcgttctg ttctatcaat tattgctccg gaatattttt caatagtcaa gctattatca
   104461 atactgacac tacagtggtt gccattccag cttggcttga tatcgctaat cacaatattt
   104521 tcatcggcat tttttatttc atttttaatc caatgtctac tgttgtcatc ttcaatcgct
   104581 tttttatatg cagtggccag acttggttct acctcgatgc taagtactgc ttcatgaaaa
   104641 tcattcatag ataaaactcc tttcaaaata aaaagaccac tcaaagagtg atctaatatg
   104701 taatagcaac ctacacacag acaagtctaa tacttcctgc gcctacccac ttcctcaata
   104761 cctcggttgg ctaaagtcac tggagtggaa tcgcaccaca cacgagaact tatcaggctc
   104821 tcacgaggct attcgccatt taccgttgcg tcttctactt ccgccacagt gacaaattaa
   104881 tattgtgaaa ataaatacta agtgtataat tttatttatc agcgagtggt ccgctgaaat
   104941 aaatatacga aggtgaaaac tatgttaacc aatgaacaac gcgctcatga tttagcaatt
   105001 gcaacattac cttttgtcag agatattgtt aaatctcaaa taattgaagg gaaagatgca
   105061 aaatttgatg catattttga atatataaag ctttataatc aatttctttc cgctgtttct
   105121 gaagatttta aaaactagga gcaaaagctt gaccattaat taaaaaatct ctactttctt
   105181 tataaatatc atcaattttt atattttgct ccttgctacc accaatagct tggagcaact
   105241 cttttatttc tcctgaagta ccttcaatcg ataatttcat tttatatacc ctccaataca
   105301 taaattaatt agctaattgt tgctcattga tctggagagc tatttcttct tctatgagct
   105361 caacgatctt cctttttgtc gaatttaatt cgaacttatg atccattaaa gtaagcttta
   105421 tgttcttaat cttttcacct tcgaattctt tgataatatc ttcgattctt tggtttacta
   105481 aaatagttag ttaatcaatt ttttcggaag caatcttcat tttatatacc ctccttaaat
   105541 aatttaatag acagcagcag ttgaaagacg acaagaacgt gtttagtttt ttgtgcgctg
   105601 ctgtctaatc aaaggcaata gaaaagagcc acagatacca attaataaaa cggttgggat
   105661 atcttggctc tttcatattt ttttgacact aatagaatat catgctgcta acaatgttta
   105721 catagctaca atgacctaac atttagtgaa cattgtaatt ttagaataaa gagagctgtt
   105781 ctttgtactt cttgctattg taaaaatcaa tttcgttttc ttttttcaat cggtcagctt
   105841 gtctgttttc atactcatct agaaagttca atgtctttct gatttctgca tgtcgttgtc
   105901 tgacatatga tgagctgtat ccaatatctt cagcaatatc ttctaagctc atttgattaa
   105961 catacttcat cttaactatt tgattatcaa taccagaaaa gctatctatt agcaataaca
   106021 tttcttcttt ttgatcaatt aatagtctca attcattttc tatcttttga atattttctt
   106081 ctaaagaagc tgatctagag tttttttcga ttcggacgtc tgctaaatct ccattgaccc
   106141 atcgattcaa ttcaagctta cttttattaa gattccactt taagtaaaga atctgctcat
   106201 ctagttcttg gtaatctttt aaccactgaa atctcacaaa cgccacccct tatatgataa
   106261 aatagtattg cgctgctatc tcgaaagagg tggctttttt tattttttct tagccgttcc
   106321 attctggatt gccttttgtt ccaaacgacg ttttttcttc ttgattttcg attttgtttt
   106381 tcccatacaa acatcctttc tggaatatat atcgttcgat ttttactgtt taacaagaga
   106441 tggccaaatt ttattgttcg ctttgaatca tctctttgaa aaaattttag tatttttgta
   106501 agacccccta aatcagcttt ttttatttga tataatatct tcacaaggta ttttacttgt
   106561 aaattaaatc gaggtgataa taatggaaat tgaaaatttt gaacaaaaaa acagatttta
   106621 tcaaatttat taattgatgg ttttgataac gcaaactatt cacataaact tttatttaaa
   106681 agcgagctag atgatgaaaa agaatttgat aagcaaaaag acttaatgtg cgccttaacg
   106741 tatttaaatc aggctcacgc cagcttcaca aatgcctaca cctttattgc tcttaacgat
   106801 gaacttcttg gtggtcgtca agaatttgac aacatattac atcagttcac tgaatttaat
   106861 acagaattct taaataatgt acgaaccaac cacagccatc aatggagtga tatagaattt
   106921 aaacgtttag tagatttctt tgaagcagca agcggtttat taaatggcca cgaaagaatt
   106981 caaggtctga ttaatgaggc tagaaaatag acaataattg tttcccagcc gaccttacac
   107041 gtcggctttt ttgctttttt taacatttct aactatttag ccgagcaact attattcgtt
   107101 acgcgatatt ctttttttat ttatcccact tggtaaaaat cattggttcc ggcataatct
   107161 gatcatcctt tagcaacgca tcaggatatt tttttcttag cttcttatta ttcgtatatg
   107221 ttttaagcaa ccattgactt gcttcacttt tgctttcacc ggaaaaataa attttccctg
   107281 tttggatgcc cgcaagttga atcatcattt ccacctcaaa ttagattttt ttctgctaaa
   107341 tggagcaaaa gtaatccgac atcaagaatc ttttgatcca ttatttcagc tactttgctc
   107401 ggcttaactc cttgagaaaa gagcaagcgt gcttgctcta tctcttcgtg agtccaaata
   107461 aatttggctt cctcaaggat aattactgga tcagttgatc tcattgttcc tctggaaaaa
   107521 tgatgtcgtc cttatgtttg gaccagctat ctgcatatgg agcaaagtat tgtctagcta
   107581 gttcgatttg atcaattaaa gcagtctctg ataattcatg atctgcagca atttcactca
   107641 tactatcgcc ttcatctaaa cggataagaa cgtctcgaat atttgcggtg acattttctg
   107701 gtagttgtaa tgtcgttgct gttttaataa atttgtcaat cgtttctttc gatactaaga
   107761 ttgttacatt ttcaacttct tctacgccat caccgacatc aagtgaagtc tgctcttctt
   107821 tgagcatttc gattgtgcca tctgaattca caacgtattc aatatttggt ttcttcgttt
   107881 gtttatttac tggcactgtg tattcgatag tttctggctg gattactaca gtcactgttg
   107941 agccaagaaa ttcagttaaa ttttcagtac tacctctcaa tgatccgttg ctaacaacta
   108001 gtaatacttc tgtgttccca tttgattttg atgttacttt ctttaattct ggtctgaaat
   108061 tcacctgttt tgtcatttta ttttccctcc attaatttct tttttcttcg ttccgtttgt
   108121 cttttccgct gctgtcttgc tgattcaaag cagtccattt caatgaattt attgatcttt
   108181 gaatcgaatt ttgcaaacgt cactggaaag ccgtatcgat gagcaaacat cttcattttc
   108241 agcatagaga tcgcatcttg ataacctttt acatcaatca cttttttcac ttgaccattc
   108301 tcataaatca caaaatcagc tcgataataa attggtgcaa tcttacgatc attgcataga
   108361 tacccttctt gcagcagaac attttcttgc atagcacagt aggtttcaga taatggcata
   108421 aaactcatac cgtttcgttt taacttcatg taataacgag cttctgcttt agaatcaaaa
   108481 gtgatgccgt ctacttcgtg ctttttgttt ccgtatttgc ttcttgttgc catttgctat
   108541 gctccttctt tagtctttct agtcgctctt ttgaacgtcg tccattttca ttgcatattg
   108601 gacaaggttc acaagttgac catccaagcg atgtcttata ccaaataact gtttcatcgt
   108661 tgcattttaa acaagtcatt ttgcacctct catccatgct tggttacttt tcggacaaca
   108721 acactctttt ttcactggct ttgtcttagg ttttgaaaat cgtctttcag gcttaataac
   108781 ttcgactact tcaccgccac aaacacttgc aacaaggtca gcctcttctt ttgtcgtgtg
   108841 gtgtttcgca aactcgacac tggcttctcc tcgtcctcgg ctatctaagt agcacatttt
   108901 tttcactaca aaggtcaaac tggactcctc cttttatctg tcgttgtttt gaatacagcg
   108961 atgttccctt tcgttccatt cattacacga gagaacactc gctcaccgta cgcgtgtttt
   109021 aattgcttcg atgataaatt agtcgtgaat atcgtagctt tgtttaaacg agcttctgtg
   109081 agcgatgtga gaacatcaac atcgtatggc gttgcttgat tgttttcttc cattcttcct
   109141 agctccgcac ctaaatcatc gatcactaca aaatccgtct tttttatttc agccatcaac
   109201 gatcctgtca cagattttct tgcttctgga tcattcattg caaatttcaa ttgctctaat
   109261 aattctcgat agcttataaa tgaacaggca actttataat tagatgccat cattacttct
   109321 ttgattactg cagcacctag atgagttttt ccggcccctg ttctaccagt aataactgcg
   109381 tgaatcgtgc taccacttac gattttttta gcccattctt gagccatcgc ttttatttgt
   109441 gctgtttcct gatcaactac tttgtatcca tcaaaatcga aggtccatat ggcatcatct
   109501 gtaatcattg aactgttaag cattcgattg atagcatcct ttttcattgc ttcattcgct
   109561 ttttggatag cttctttttg atttttagtt accatttctc gataaccaca aatcttattg
   109621 atacatgtag gaccgcaacg atcagtacca tctttatttt ttgttttcca gatatacaat
   109681 ggatcaccgc attttgggca attatgatct gctgttttta atccgccgta aggtgcagcc
   109741 attgcactta gttttttatt cattaccaat cctcacctgt ttctggtaac tcatcattag
   109801 aaatattttg ttgttttctt ttgttttttt ggacagccat cttatcaaaa tgttttctca
   109861 aactcgttgg agataagatc acacctgacc agaagtcatt actagtggcc cacacaatca
   109921 tgtcttgaac ttctctacca gttcgtttgt cagattcaat tgttaaacga atcgtattcg
   109981 cccatttatc caaatcaggt tctttgatgt tttgattttt tcgaatgagt tttaataaaa
   110041 gaatggccaa ttttttattt ggatcgtcat ctgcatactc tcgtgttttt cgagagttgc
   110101 gaagattatt attattctta tcattcttct cattcttttc attcttgttt atgttcagtt
   110161 cgttgtcctt ttgatgttca gctcgttgtt cagtctgttg ttttttttct tccgaaaacc
   110221 cttgataaat ggcgtagtta ttgactttat acgttgttcc atttttcctg cttttttcaa
   110281 tgcttatcat gtcatcttca acaagtagac ttaaaaattt tctgacagta tttctactta
   110341 ctccccaacg ttcagccaat ttttgttctg aagtaattct ttgcccaaca tcaacttttt
   110401 taaatgaacc attaaaaaga attttctttt ctttgtggtt ggccattaga attaaatcaa
   110461 gccaccactt tagatattgc ggattgtccc atatccaatt ttcagttata gaacggtgaa
   110521 gcttgaccca tccttcagcc atataatcac caccgttcta taaatcgtcc atgctagtga
   110581 atccagtaat tggaagatta cctctgcagt actcacataa accgcaatgg ttcggcttct
   110641 cttctccgtt cttcactctg acaattcttt cgattttttc ttttaacata accagctcat
   110701 aatccatatt gtcctggtca atcgtgatca gtcttgcttc acttggtgtc tgcttggaca
   110761 ctgcagcgat gatcggcata aaactttttc cgtattgttg gaaaagtaat tcttgataaa
   110821 tcgccatttg aagcacgtaa ccaaaattga caatgaacgt ttctcttgat ccgtagtttg
   110881 cgttccattt acgctcgtgc atgtctttgg ttgttttgat gtcaacaaaa tactcttcct
   110941 ctacattcag acaatcaatt ttgcctttcc atgttgttcc aaacaattca ccggtcacaa
   111001 tcgtttcttt ctcaccttga tagatattca tgaatgcttc ttcttgctta agtcgttcaa
   111061 tcatttgttc ggcaatctgg aaatctttta acaatccata gggcttccga ctagaaaaca
   111121 tttttgattt attttcttct ttgaacgctt catgtacttc ttttgattca aaataagaat
   111181 gtacgtagtt tcctacgagt aaggctttgg gatccgacgt cggtgaccaa tcgcctttca
   111241 actttgctaa agctgcggct tcgcatttta aaaaggactt atattgtgat gttgacatgt
   111301 actgccagtc agcttcattg gagtaataat tttcatcaga aagggtactc gtcgtcttca
   111361 aattctgaga catcgtctac accttcctca cgtccaaagt ccggaatatc taataattct
   111421 tcttgaatcg gatcggtatc ctcattttcg attaaatgtg cttgttctat ttctgaatgg
   111481 ccaggtacgt cagcaactcg agttacttca tcttggatat catctggaat cacaattgga
   111541 tctggttctt tttgtattgg ttcagtaatc tttttgtttg aaaagaattt ttgttcgaga
   111601 gtagccactt tttctgtttg tggcgttaca tctttccgat cattgtcaaa ttcgttttct
   111661 aacgtatctt ttgcagcttg aactaacaaa tcgttatcgt tgcttgtgtt gattaatgct
   111721 ttagctgctc gattaatcac tgtccgttta gccatttctt ctggaaattc cttttgtggt
   111781 ccagaatcct tcatttttga cttcgcccaa ctttgatcga tttgcttttt cgtcatgact
   111841 gttgtggttt ctttaccatt tgctaacttg ataactacat aggctgcaat gatttcattg
   111901 tctaaattct cgaattttgt ttcatgcttt ttgacaacca tgttcggtcc atccattgca
   111961 acttcaaact catcaccttg tcgaacgact acaggcgtaa tatctgcccc tccagttaca
   112021 cgatcaagga ccgccattgt tccaaaatat gaacgcatta gctgaacctt gtttccgtat
   112081 ttaatgaaat agcattgttt ttttgctgga gataaccctt gaataaccat gtctaaaagt
   112141 gcattagaaa tggaggtttt catttcttga ttgtttgcag ccatctgcaa caaatttcct
   112201 cctgtattgt tagtgagttc gaaaaaagca ctcttcaacg cgttttgcgg actatatcct
   112261 ggtggcattt ctaagccttg ttcttgtaac cgtccaagat tactgatcac ctgttcatct
   112321 aatgaacgtt gtgttgtttg tgttaaatca tttgccattt ctaaattcct cctaattcaa
   112381 tctttggata atgttgctgg atgtactcta gaattgcgtc tactggcaca tagccttctt
   112441 ctgttaaata gcaagaatcg ccttgataca aagaatttcc ttgccaatcg actccaacag
   112501 gactagcttc atttggtggt tgctgtcttg cgcctaaaga atcaaagtct ttcatcacag
   112561 cctcctaaaa ttcccaaaat agttttaatg tgtcattgtc ttgttcaatg ctagatacgc
   112621 cttctgtttc caatgccgct aaaaatgaag gtgttagccc ttttgagcga atcgtgcaac
   112681 tcgtttgacc ataactagca gcagtcaaaa tcttttcttg tacatcttgt tgtgctttgt
   112741 gcatcattgc ttcgaatatt tcatcgccta accctttgac tgagatcatc agaattttat
   112801 accacctttc ttgcgatcta ttttttcggc aaaccgtaga aagtcctgat accattcctc
   112861 aaactctgga tcttcatatt caataccaaa caattgtttt aaagtaccgt gagtttcaca
   112921 attgcattta ttattctgca aatcacgttt tgttttctca tactgttggt aaacactcca
   112981 cgcaaccaag ctatcgtttc cagcttgtcc aagagcgact tccccttttt catcgactac
   113041 actaactgac attcctagat catcattcgc acattcagct gctaactctt taattaaatt
   113101 ttggattttt tcattcattg tgataaactc tccttgtaat tagtattttt catgacacga
   113161 ttttgcttgc cggcggtcgt gtcttttttt gatagctata tcgagcagct atcagtttgt
   113221 gatgattatc aatttgtttt ttcattacgt cataccagtt ctgactagcc tttgtacgat
   113281 cgggttttat agttatttct ccttgtttcc ttaatagaca aatttttctg aaaagctcgt
   113341 gctttggtct atgcagtagt ctagttaaat aatcaatatt taatacgtaa ccatttttat
   113401 cgaatttaat ttctgatatt aataactcaa cttctctttt tgtgtacctg cgttttcgat
   113461 tgctttctaa ttgtccattt ttcttcatga tattaagacg acagcgaatc gcgttctcag
   113521 ttctattcaa ttcctgcgct attattgtta ctggacaacc tgactgatac atagaaatta
   113581 tttttttatc ttcttgctga ctgtaacgtt tgttaggcgg ttcgattgga tcatcccaat
   113641 aaacttcatc gatcaaatct tttttacgca tttgatggat tttgggccaa atacgctttt
   113701 tatctatccc aagtaactca gctaattcat tacaattaca aacaaatccg cgatggtcgt
   113761 atctgacatt ttgaataaga agttgctcct cttctcttgt ccatttgttg tacgacaatt
   113821 ttctaacctc ctttttttat aaaacggcaa agacaacaga caataaaatg acgtttagaa
   113881 ctaagctgat atagcaaagt gcttgcagct gacgagcttt gtatactggc tttttgtgtt
   113941 cggcaagtgt tgcgagcagc attttgctac tcgagcgctc aacgattggt tttcttattt
   114001 gcatagttga ctccccttcc ggaaaatatc gatcaataat acgttgtgcc tcttcttttg
   114061 ttttcacggt atgagacata tgtccgtctg tgatggtgat ctctgtcatg tatgtcctcc
   114121 ttttagattt tgtatttagc catcagttca tcgatgtctt ttttgtcata ccttaaaaag
   114181 ccatctatac gaatttcttt taacccatga tctactagct tctcaaagcc ttgatcattt
   114241 acacctccca catactttct agcttcgatt tttttcaaat atcgtgtttc tgaaagattc
   114301 ttttttgaaa gctgattaag tgcgtccttc acaacttcaa caattgcaat tctcaaacga
   114361 gtcagaaact cttcgcttag aatattcatg acttgcctcc tatcgaattt tatttttctt
   114421 tccaaactcc atacttaata gcgaaatcct tgactacaga tagataaatt tcttttaacc
   114481 ttttatcgct ttcgattaca tctaacttat ttgtcttatc tcgcttggat tttggaacac
   114541 cttcgccagc catacggtta cgtaaattgg ttaatctagt tttgagagag gagccagctc
   114601 tacgatcaac ttcttcatat atcgctgttt gaatttcttg ataagcaccg tatccgcctt
   114661 gagtttccgc cattttattt acgagctttc gacattctct acgccagtca gttgtattca
   114721 atgccacaat ttcagtaata ttctccattt gtgttgctag acgttttgtc tctaactctt
   114781 gcttagctac tgcgttaaat attccttgga acatttgaag ttctggactt agctttgaag
   114841 tatcaagtaa ttgttgtttg tattcttttt ctacttcaat aaaatattga cgcgcttgtt
   114901 ttcctttttc tgttcgttga atcattgaga tttcttttgc catgtccaat ttcattgcat
   114961 ggtcggtaat attctgaatt cctccagggg tcggacattt ttgggtcacc cttacgaaat
   115021 ctatattttc aacaaaacca tattccgcca tacgactaaa ccattttttg tattctgttg
   115081 ccacctccaa aaactcatgc aattctctgc cgtttactaa ctgctcattc tcctcatttg
   115141 ttgttacttt gattagttct ttcatggatt acactcctta aaaatgaata aatatgtttt
   115201 ccataagtta ttttattaaa aatgaatcgt tatgaagttt atatcgtata aactcgtctt
   115261 tgcttccaaa aaaaatattt gctgttggaa aatggtatat ttctggtatt aattgcacat
   115321 atttttgctt cattttattc ggatcttgtt cccaatttgc taaggtttga tggtgaacat
   115381 caaagagttt tgctgcttct tttaaagaat atcccatcat aactctagct gcttccaaag
   115441 taatttgtaa ttccattttt attcctcctt cgtttatgta aattatttta tactagttta
   115501 tactaccagt caacgatttt tgacgttttt taaattaaaa aacactgtta taaaactact
   115561 ttatacttgt ttatattgat ttaattcgat attattaata aaaaaggagg agaaaataac
   115621 atgccaagaa aaccattatc agaagaagat aaaaaagctc gctccattat cgctaacaat
   115681 ttaaaaaaaa ttacgcatgg ctacacaaga gcacaattgt cagatatgac aggtattcct
   115741 gttacgacgc tatcgggata ttttactgaa aaatcaacta tcagtatgga aaacgctgaa
   115801 ttaatagcta aagcttttaa tgttgataaa tctgatatag atccaagata ttccagagaa
   115861 tacaattatt atgatttaaa gaaagaagtt ttagatgaat tacaggaaaa caataaaagt
   115921 ttcgttgctg ataaatttgt agagttagca gaaaaagcaa atgcagcagg gatgactttg
   115981 aacagaacta aaactgatag aggaaacatt atgacagttc tatcaagtga tattcaagaa
   116041 tataactttt ttgacacttc tgttgctgcc ggcatacctt cttcagtcga agcttttgat
   116101 caagaccata ttgagcaaat tgctattcct gattatgcca tgggaaaata tgcaggatat
   116161 caagatgtat tttttactaa aataaatgga gattccatga ataacgttat tcctgatgga
   116221 tcattaattg ctgtaaagaa aatagagagc tgtgatgagt taaaagatgg agatatagta
   116281 gtctttagca acgataacga attttctgta aaaagatata taaatgatca acaaaataat
   116341 cgcatcattt ttagacctga ttcttctaac atttctttca ctgatattgt ggttaattat
   116401 gatgatacta aatacgttca aatttatggg aaagtcgttg tatatattgt ccaactataa
   116461 ttaatttgag agggtataaa atgaaaattg gaatgagaaa accgagcata aagaaatcta
   116521 tcggcgctag aactactgga aaggcaaaaa gagctgtgaa aaaagctgtg attcctggtt
   116581 atggaaagaa aggctctgga tggattaaag acccaaaaaa agctgcatac aataaagttt
   116641 ataaaaagac tacgtttagc ttttgggact tatttaaata aaaaaagccc gtgcagcaac
   116701 acggactcga aaccttattt ctaagattcc ccaataaaat aatatcatag agataaggag
   116761 tagaaaatga aatttattaa atttggaatt attggattac tagtagtgac agttatggga
   116821 ggatgtggaa catcaacagc tgaaacaaag gaatcaaaaa attatagtga gacaaacagt
   116881 actatcaaag aagtagactc tgatcaaacc gctgagtttg caactattgc cgagaaacac
   116941 attaaagaaa tatattcaat agataatttg aagatggatt taaaatctat caaagttact
   117001 caatttcctg acgataaaaa tgcagaaact ggtgaagagt acaaaaatgt ctacaatggt
   117061 actggagatt ttacttggca agacaaaaaa tacaactttt ctttgattta ttcgaaaaaa
   117121 gatgcaacta attataccgt cctatatcta tatagcaatc tagatcctaa taaaggtgta
   117181 gatacaccat tagaaagcga tcaataaaaa aagcacattt accgcccgac caagaacgca
   117241 aatgtgcaaa gagctacggt aaagtaggtc tatttgttgt accctatttt accatctcga
   117301 aatccgctgt acaaccgaac aaacgtacga aaggaaagat aaaagatggc aaaaaaagaa
   117361 attgataaac gaattaaaaa gcacttaaca aaaaaaggag aagaaaaata tcagtttagt
   117421 ctgtacttag gagttgatcc gctaacagga aaaaaaagaa aaacaacgag acgaggtttt
   117481 agtacgccat tagccgctga gagagcttta aaaagattgg aggcagatat acaggaaaaa
   117541 ggactgcaag cttctacagc tcaaaagagc aagaaattcg aatatgttta cggcttatgg
   117601 tttgaaaatt ataaaaagac agttaaagaa agcacttggt cctccaccaa acaaattttt
   117661 gacacacaca tcttaaaagt gtttggagat aagttcattg acaaaataga cgtttttttc
   117721 tgtcaagaag cagtaaacac ttggtccgat agccatccta aaatttttaa gaaaataaaa
   117781 aattacacat ctaatgtttt tgattacgct gcttctttac aaatcataac tagcaaccct
   117841 atgaaattag tttctgttcc acgcggagaa gctctagata tcgaagataa aaatatcgaa
   117901 ttttatacta aagaagaatt aatcgaattt ttagaagcaa taagaaacga tgatgatgaa
   117961 agatatttat tcttttctct attggctttt acaggtataa gaaaaggaga agcttttgca
   118021 ctgacctggt cggatattga ctttaagagc aaaacactca atattaataa aactgtaaca
   118081 agaggatatc aaggaagatt gatcgtaaat actcctaaat caaaatctgg aaaaagaaaa
   118141 atttatctag acaatgattt gatcagttta ttgagaaagt attatacaaa aaacaaaacg
   118201 atcgttacaa tccaaagcga aaacttaatt tttcaacatg atggtcttct ctataatcct
   118261 acagtttcgc gttcgtggct taatgtcata tataagcatc atccagaatt aactaaaaga
   118321 atcaccactc atggttttcg acatactcat gcttctctcc tttttgaatc aggagcctct
   118381 ttaaaagatg ttcaggaacg tttagggcat gcagatattc aaactacttc aaacatttac
   118441 actcatgtaa ctgaaactca aaacaaaaaa gttatcaata attttgtagc atttatgaag
   118501 aatagtactc agggggagtc aaaaggggag tcaaaaaata ttttgggcta aaaatacaac
   118561 aaaaaagaca cgatcgaaat cgtgtctaat cgttgatata taaggaaggt acaggatttg
   118621 aacctgcgcg ccggtattag ccggttcgcc ggatttcgag tccggtgcat taccactctg
   118681 ccaaccttcc gtaagtacta tttttaaact tttttcacga aatgtcaagc ctatttctaa
   118741 tagataaata acagaaatcg ctttttttag caaaaaagtc cattgacagt ttgcagccaa
   118801 cggacttacc gatcaaattc atactgatag aacactatct gattatttca ggattatcta
   118861 atgctaaaat cgagtcaatt gtccgtttga tcagtgcaat gatttgttct tctgttggat
   118921 gcatgatgtt tgatgacatc aatgacgcta agccagtcac tgctacccat gtaccagtat
   118981 gcagagcatc gacatattca tccgataatt gattgtatct ttcatcttct ttaactattt
   119041 cacggaaata attgtatgaa aaatcatgca ttttctttcc gtccccatgt tcatcaatgt
   119101 acaatgccat gtaaagacta tgatgctttt ttgcaaagta gatatagttc aaaccgagat
   119161 caatgatttt atcaccagta cgttcaatag ggaaaacatc ttcttttagt tgttcaaaga
   119221 tagaatccag caacgtattt tttaaatctt ccatgttttt gaattccaga taaatcggct
   119281 gtgtcgaaat ccccatcttt ttagcaatat ttctcgcagt aaatccttta aacccttcct
   119341 ttgcaacaac atcataagca gcttttagaa tttgatcctt ggtatagact tttcttctca
   119401 ttaaaactcc cccacttctt tactgcagta tatgttgttt tattgtaccc tatttttagc
   119461 attttttgca aacattttac tcaaaacgtt atattttaaa ttttttttca gtcttcttta
   119521 actttttttt gcaaaattaa aaaaatttgg ttaaattcaa tgggttccct tacattttta
   119581 gttttgacct aaatgatagt tgtccaaatc tttcatttca agttatagtt aaagaaaatg
   119641 aaaaagaagg tgtgtaatgg aaaacgaaac tgacgttatt tatattcatc ctcaaaaacg
   119701 aatcgtttca cagaaaagaa agtattttta tcttggtttt acaggtgttt tttttctttt
   119761 tattggcttg ctttctaata ctcctactga taattggagt ggattgctaa ctattcttac
   119821 ctcgccaagc aatcttttga cagattattt tgctttaggc ggtttcggaa gcgcattcat
   119881 aaatgtaggg attttgacct tactttctgt gcttttagct tatcggcaca aggtgatttt
   119941 aaacggtccc ttatttgctt cgattctaac agttaccggt ttctcttttt ttggcaaaaa
   120001 cctttataat tctatctcaa tcattttagg tgtgtatcta tacgctgtat ttgtcaataa
   120061 acctttttct cagtacatca tgatcggact gtttggtagc gcattatcac cggtcgttag
   120121 ttatatcact tttggtatgc gttttccttt actgataggt attttattgg ggaatttagc
   120181 tggcatagca attggtctac ttcttccgcc cttagctgct caaactttag ttttccatcg
   120241 tggattcaca ctttataata ttggattcac ctctgggttg attgcgatga cttttactgc
   120301 tgttttgagg ttattcagct attcaatcgt cgaaaataca ttggttttca atgaatatca
   120361 ctttcctctc atttggatta tttttggctt tttttcgctg acagtcggaa ttggtttcta
   120421 ttataattcc tttcggcttt tggggattcg agaaattttc gattcatctg gaaaattgac
   120481 aactgatttc attgctaatt ctggtattgg tgctactttg atcaacatag gcttagttgg
   120541 tctgatgctg tcttcttatg ttttgctggt tggcggtcag ttaaatggac ctgtcatcgg
   120601 tgctatttta tctgcggtgg gtttcagtgc ttttggttgt catttaaaaa acagttttcc
   120661 gatactagtc ggaatattta ttgcttcttt gtttggaact tttcatgaga tcaccagtac
   120721 tggcatgctt gttgcagctg tgtttgggac aggtctcgcg cccataagcg gtttttacgg
   120781 cagtttttac ggtgtaatag ctggtgtact acatattgct cttgtccata acgtcagcac
   120841 tttgcacgga ggactaaatc tatataacag tggtttttca actggtttcg ttgcaggtat
   120901 ccttgtaccg atactagata actttaccgc tgtcagaaag gaaaaaaaga cacttggaaa
   120961 aagaattatc aaaaagaatc atcgatgagc ttcttactta tttatattcc catgaaataa
   121021 ctgaaatacg tattggagta gaatttgctg aggaagggct tttcatagag atacagggta
   121081 cggcagaaac tcagccagaa gatttagatg tacttgctga tttgctgaac aatcctcggg
   121141 accctagtat ggaaatctat tatcaagaac tattaggtaa tccccgacat gatcgggaag
   121201 attatcatct gttaggtagt ttgattgatg acgtggaaat catgtatgcc cagccgatta
   121261 tcaatattaa gatctttcga cgtgcttctc attcctatta ttaaccctat tttctattta
   121321 taaaagactg aatcaaaaaa gcagctcctg aaagttcgat taaaatctac tttcaggagc
   121381 tgttcaagct tgacattatt gattttccaa tccttcatac acttcatctg ttacaggttc
   121441 taaaaactct gaattaccag cagtgatcgc caaatgttca aaccaactgt cttttgtcgc
   121501 accatgccaa tgtttaatcc ctttgtgcgt tacgacgaca tctccagcca ttaatcgtct
   121561 tgctggcttt ccttcttcct gataccagcc ttctcctccg gtcacaagta agatttgata
   121621 gccgtcttta tggacatgcc agtgattgcg acaacctggt tcaaaagtta cattacctac
   121681 accaacagaa acttcgggat cattcactaa catattcaga tagctttgcc cggtaaatac
   121741 ttgagcgtaa gcttcgtttt tttcacctgc tggaaaaatc acgccattgt gtatttcttc
   121801 gtgttttgtc gtcatttacg cctctttctt atcttcgcta ttttcttcta aatagatttc
   121861 ttttgctaaa ctaaaagctg accaagcttt tggccatcct acataaaaag ctaaatgggt
   121921 aatgatttct gctatttctt cactagtaat gccattttgc ttgccaatct tcagatgagc
   121981 tggcaattgt tcaaaatttc ctcctgaaat taatgcagat atggtgatga tactacgctg
   122041 atgagcagat aattcttctc tggaccatac ttgtccaaat aaaacatcat cattcaattc
   122101 tgcaaatttt ggtgcaaaat ctcctaattg atctcgtcct gcggtttgtt tttctgccat
   122161 ttctcttatt ctcccttcac gactgttagg tgattcaact atggttctaa ggcaacttta
   122221 ttttctattg tttcttcgca tttcatttta aatgtagtct ttcagcctgt ccaatgctta
   122281 gtttattcag tcgatatgct ttttaagcat agttgctaac gcttttttta
//
LOCUS       DACGLE010000002       113317 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0534
            SAMEA3304449-rid9560453.denovo.002, whole genome shotgun sequence.
ACCESSION   DACGLE010000002 DACGLE010000000
VERSION     DACGLE010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304449
            Sequence Read Archive: ERR985064
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 113317)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 113317)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702225.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702225.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 20:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,045
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 27 of 67
            Pseudo Genes (incomplete)         :: 25 of 67
            Pseudo Genes (internal stop)      :: 19 of 67
            Pseudo Genes (multiple problems)  :: 4 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..113317
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304449-rid9560453.denovo.002"
                     /strain="VREN0534"
                     /db_xref="taxon:1352"
     gene            complement(218..1015)
                     /locus_tag="IXT20_000134"
     CDS             complement(218..1015)
                     /locus_tag="IXT20_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HAQ4858614.1"
                     /translation="MMKKFFLFFLLLVSVGWPVATHADTATVNDEAGLFTTDQIQSLE
                     QQAEKLNEKIKGRVLIVTTTSNSEEPRDFADNYLRDAVGNDQNGSVLLLDMGQREIYI
                     STSGNMIDYLTDSRIDSILDDVYDQMTNAAYYNAAKAYLSKSATYVEDGVPGGHYRVD
                     EETGKITRYKVLTTVEIIIAVVLALILSLVFYFVTVSRYQLKSGIYKYPFREKSSIKL
                     TEKTDRLTNSFVTTRRIPKSPPPGSGGGGSTTHSSGGGTFGGGGRSF"
     gene            complement(1012..2196)
                     /locus_tag="IXT20_000135"
     CDS             complement(1012..2196)
                     /locus_tag="IXT20_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HAQ4858615.1"
                     /translation="MDVLTHKCPNCGGPLTFDPKDQKFHCEYCLNIYTEEEVSQYEQK
                     QKEARMAGEESKEEPSPTEDFTFTAQEQLADMNEAERKAVDEAGGFSDTADESADNTA
                     AIDLFLCPNCGAEIVTDATTAATYCYYCHNPVVLSGRLSGEFLPNKVLPFAVEKEEAI
                     NRFLAWTKKKWFVPKAFFNKDQIDKLTGVYFPYWATDAEVDGAMQANGTVIRIWRIGD
                     IEYTETKQFAVSRAGKLSFKELVKNALSKNTQQKMVEGVQPFPLDKAIDFKSQYLAGF
                     QAEKRDIEYEAIKSQIQSELKDYSEKLLKDTANGYTTLTGVRTSASITNEVNNYVLLP
                     VWLVTYRSNDSSKKVYYYAMNGQTGKVSGVLPISQKKLGLTALGIFAGLAILFLIGGY
                     LI"
     gene            complement(2221..3468)
                     /locus_tag="IXT20_000136"
     CDS             complement(2221..3468)
                     /locus_tag="IXT20_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virion core protein"
                     /protein_id="HAQ4858616.1"
                     /translation="MGLIKAATSMVGGGLADQWLEVIEPDNMSDTTVMTKGVKVRKDD
                     KRGSNRKGTEDVLTDGTVVHVYPNMMMLLVDGGKIIDYTAEEGYYTIKNDAAPSMFNG
                     TLKEAIAETFDRFKFGGVTPQKQQVFYINLQEIKGIKFGTSAPLNYFDNFYNAELFLR
                     AHGTYSIHVVDPILFYTNAIPKNKTQVEINDINEQYLAEFLTALQSAINQMSADGQRI
                     SYVPSKSLELSKYMDTALDDSWRELRGMEIVSVAVASISYTDDSVKLINMRNEGAMLG
                     DPSVREGYVQGSIARGMEAAGKNEAGAMTGFMGVGMGMNANGSYLSQAAQNNQEQIKQ
                     QAEKQNQQTAQGNADTWTCPVCGTENSGKFCSNCGAAKPVENVQPKLQMRCSECNEIV
                     DLSNGIPKFCPNCGKPFKGIPVN"
     gene            3683..5428
                     /gene="dnaX"
                     /locus_tag="IXT20_000137"
     CDS             3683..5428
                     /gene="dnaX"
                     /locus_tag="IXT20_000137"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HAQ4858617.1"
                     /translation="MAYQALYRVWRSQRFEDIVGQKAVTQTLKNAIVSHKTSHAYLFT
                     GPRGTGKTSAAKIFAKAINCPNSKDGEPCNECEMCRSITAGTQEDVIEIDAASNNGVE
                     EIRFIRDRANYAPTKAAYKVYIIDEVHMLSTGAFNALLKTLEEPKESVIFILATTEPH
                     KIPATIISRTQRFDFKRINTADIVEHLEFILKESTISYEPAALNVIARSAEGGMRDAL
                     SILDQAISFSEGTITLDDAMQVTGSLTDEMMDSYLSACVKKEVPEALETLNQILAAGK
                     ESRRFLEDMLQYCRDLLMYQQAPNLVEEQSSTIRSTFTELAQTVSADQLFSMIKILSD
                     TQNEIRFTNSAAIYLEVATVKLAQLPSASVPSADVAFADDQIVKQLQQEIKELRNEIK
                     ELRSNTGIENTKVQPVQQTNHKKQTSTYRVPTERVYKVLREATRQHLNQVKEVWEDLL
                     MSLSVTQRAMLKASEPVAAGPTGLVIAFDYEIVCQRAANDEELQLSVHNNLSRMIKDY
                     APDPVYITRESWPELRRNYLTQAQEEGTENSFASEDSEGEIELLPQEQEVPEETLVVK
                     AEELFGSEAVTVVDD"
     gene            5448..5762
                     /locus_tag="IXT20_000138"
     CDS             5448..5762
                     /locus_tag="IXT20_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HAQ4858618.1"
                     /translation="MMRGMGNMQGMMKQVQKMQKEMAEAQELLNKTEFTGKATNELVK
                     AVFTGDRRMKDIQINEEVVDPDDIEMLQDLVVMAVNDAIAQIDVETEKKLGKYAKGLP
                     GF"
     gene            5787..6383
                     /gene="recR"
                     /locus_tag="IXT20_000139"
     CDS             5787..6383
                     /gene="recR"
                     /locus_tag="IXT20_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HAQ4858619.1"
                     /translation="MQYPEPIAKLIDSYMKLPGIGQKTATRLAFYTIDMKDEVVNEFA
                     KSLLSVKRDLHFCSICGNITEEDPCEICRNPSRDRSVILVVEEPKDVMAMEKMREYHG
                     LYHVLHGVLSPMEGTGPEDINISSLLQRLHDADVKEVIIATNATTEGEATAMYLSRLI
                     KPAGIKVTRLAHGLSVGSDIEYADEVTLLKAVEGRREL"
     gene            6447..7091
                     /locus_tag="IXT20_000140"
     CDS             6447..7091
                     /locus_tag="IXT20_000140"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HAQ4858620.1"
                     /translation="MNGLFITIEGPDGAGKTSILNELYPLLKEVAKTEIIQTREPGGI
                     PIAEEIRAVILDPKNDRMDERTEALLYAAARRQHLVEKVLPALAKGKIVLCDRFVDSS
                     LAYQGAGRRIGVTEIARLNEFATEGTTPDFTLYLDVDSDTGLRRIKKNRQNQIDRLDS
                     EGLEFHQRVRHAYLKLAEENPERIHKVDARKSFEEVLQTSYHTIIEQYPQFFEN"
     gene            7106..7435
                     /locus_tag="IXT20_000141"
     CDS             7106..7435
                     /locus_tag="IXT20_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4858621.1"
                     /translation="MKIILAIIQDKDSNRLSNELIDANIRATKLSSTGGFLKAGNSTF
                     IVGIEDDRVEEALEIIKKTCESRKQFVSTPVTLDISMDGGVPYPVEVEVGGATVFVLP
                     VEGFHQF"
     gene            7449..8387
                     /gene="holB"
                     /locus_tag="IXT20_000142"
     CDS             7449..8387
                     /gene="holB"
                     /locus_tag="IXT20_000142"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HAQ4858622.1"
                     /translation="MNQEINLAQMQPIVYQQLQRSFEHGRIAHAYLFEGEKGTGKHEV
                     GIWLAQHLFCTQMKDNLPCGKCNNCQRIEKKEHPDVLVIEPEGQTIKVDQIRRLQTEF
                     SRSGYESRKKVFLIKEAEKMNSSAANSLLKFLEEPPGDFLAVLETDAVGRILPTIQSR
                     CQVLHFQELSKEALIHRLQEEQIPLEKAKLLAFLTNSLGKAVEISQDEWFNDAKDLIY
                     QWFVYLQKQDTQAFIYVQKKLVKSFKEKTQQFTGLSVLMFYYQEAQRQAVANGEYGKV
                     KQINQIIERILIAEQKLRSNVSFQAVAEQFVLHTIF"
     gene            8423..9247
                     /locus_tag="IXT20_000143"
     CDS             8423..9247
                     /locus_tag="IXT20_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage 0 sporulation family protein"
                     /protein_id="HAQ4858623.1"
                     /translation="MVEVVGVRYRDAGHIYYFAPGDSEYVYSDKVLVESQQSLHIATV
                     VLPKKEIDPNDLPEAIRPILHKASQAELEKDKKNKKDAYDALKIAKEKVQLRELEMKM
                     VKAEYTFDRNKLIFSFTADGRIDFRELVKDLAAVFRTRIELRQIGVRDEAKIIGGIGP
                     CGRPLCCSSFLGDFVPVSIKMAKDQSLSLNPTKISGLCGRLMCCLQYESEEYETAKKE
                     LPDFGKVIATPDGKGKVVGLNLLSRIVSIRLTGRENPVEYEWDELKEFIQVGEMNG"
     gene            9240..9587
                     /locus_tag="IXT20_000144"
     CDS             9240..9587
                     /locus_tag="IXT20_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication initiation control protein YabA"
                     /protein_id="HAQ4858624.1"
                     /translation="MDKRSLYDGLNQLESELRNTLTELVEMKHALHEIVEKNTTLEME
                     NQRLREHLRELNQVAQAPAENVKQELSKSRMNLEKIYEEGFHVCYDLYGSRRDNDEPC
                     AFCLEVIYRESGR"
     gene            9656..10528
                     /gene="rsmI"
                     /locus_tag="IXT20_000145"
     CDS             9656..10528
                     /gene="rsmI"
                     /locus_tag="IXT20_000145"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HAQ4858625.1"
                     /translation="MYNQKSFKGNPVYGDLYLVPTPIGNLEDMSYRSVRMLQESDLIA
                     SEDTRNTQKLLNHFEIHTPQKSLHEHNYKERVPQLIALLKEGKTIAQVSDAGMPSISD
                     PGHELVLACIKEGISVISIPGPTAGMTALIASGLVPQPFLFYGFLPRKKKEQKETLKK
                     LKEQTVTLIFYESPYRITATLTNFLEIFGNRQVVLCRELTKVHEEYLRGSTEELLAYI
                     EEHPVKGECCLLVEGNLFEMPVTEVDEELGTLKEQVEAKIASGEKPNAAIKAVALKNG
                     LKKQEVYRQYHELD"
     gene            10637..11758
                     /gene="dinB"
                     /locus_tag="IXT20_000146"
     CDS             10637..11758
                     /gene="dinB"
                     /locus_tag="IXT20_000146"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HAQ4858626.1"
                     /translation="MMELQFPLHNDTSRKIIHIDMDAFFASVEERDHPEFRGHPLVIA
                     RHPSDTGGRGVVTTANYEARKFGIHSAMSAQKAYELCPQAIFKPGDHHKYSDISKQVR
                     EVFYRYTDIVEPVSIDEAYLDVTENKINCKSAIKIARMIQADIWESVQLTCSAGVSYN
                     KFLAKLASDYQKPRGITVVAPQDAVPFLKALPIEKFHGVGKKTVPRMHELGIYTGKDL
                     YECTEMMLIRNFGKMGYSLYRKVRGIHDSPVNVTRERKSVGKEHTYGQPLQTEEAVLT
                     QLRQLAEKVEEALRRVQKHGKTVVLKVRYTDYSTVTKRVTLPEYIYKKEALFYQASLI
                     WEEILGVEKGIRLLGITLTNLDPMTYENIVLPLWENQEI"
     gene            complement(11812..12414)
                     /gene="nrdG"
                     /locus_tag="IXT20_000147"
     CDS             complement(11812..12414)
                     /gene="nrdG"
                     /locus_tag="IXT20_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HAQ4858627.1"
                     /translation="MRNPKPKEWLAKDYSQNYIADYKPFNFVDGEGVRNSLYVSGCLF
                     ACEGCFNKAAQNFRYGKPFTPELEEQIIADLRNDYVQGLTLLGGEPFLNTDVCLQVVR
                     RIRKEFGTTKDIWSWTGYTFDELLQDSEDKLELLSQIDILVDGRFELSKRDLKLQFRG
                     SSNQRIIDVQKSLESNQVVIWEKCTDATETYEQIKKQDLI"
     gene            complement(12729..14918)
                     /gene="nrdD"
                     /locus_tag="IXT20_000148"
     CDS             complement(12729..14918)
                     /gene="nrdD"
                     /locus_tag="IXT20_000148"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HAQ4858628.1"
                     /translation="MKVNYEEKTVVTQQPETSQMKVIKRDGRHVDFDEQKINEALIKA
                     EKKIHGSLSPLTYEKIQMIADLVVQEIKSRFAENVKIYEIQNIVEHILLEKNEYELAE
                     EYINYRTKRDFARSKATDINFSIEKLINKDQTVVNENANKDSNVFNTQRDLTAGIVGK
                     SIGLKMLPPHIANAHQKGDIHYHDLDYHPYTPMTNCCLIDFKGMLNNGFKIGNAEVES
                     PKSIQTATAQISQIIANVASSQYGGCSADRTDELLAPFAELNYKKHLKDAEEWIDSPE
                     RQKEYAKAKTKKDIFDAMQSLEYEINTLFTSNGQTPFTSLGFGLGENWFEREIQKAIL
                     QIRINGLGSEKRTAIFPKLIFTLKKGVNLNPEDPNYDIKQLALECATKRMYPDILNYD
                     KIVELTGSFKVPMGCRSFLQGWKDENGQEVNVGRMNLGVVTLNLPRIAIESKGDQNKF
                     WQLLSDRLEIMKDALLYRVERCKEAIPANAPILYMYGAFGKRLSRTDSVNELFKNRRA
                     TVSLGYIGLYEVASAFFGGEWETNPEAKAFTLDIVKELKANADAWGDEYGYHFSVYST
                     PSESLTDRFCRLDTEKFGIIENITDKEYYTNSFHYDVRKNPTPFEKLEFEKDYPKYCS
                     GGFIHYCEYPMLQQNPKALEAVWDFAYDKIGYLGTNTPIDHCYQCGFEGDFKPTERGF
                     ECPECGNHDPKTCDVVKRTCGYLGNPQARPMVHGRHKEISSRVKHMK"
     gene            15244..16011
                     /locus_tag="IXT20_000149"
     CDS             15244..16011
                     /locus_tag="IXT20_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ4858629.1"
                     /translation="MEKMIEVKHLSKSYGSKLSPVKALDNISFTVEEGEFVGIMGPSG
                     AGKTTLMNILSTINLPTMGSVSIQGKEITKMKNSELSDFRRKKLGFIFQEFNLIDTLN
                     AKDNILLPLAVERMTKEEMEKRVRHVAQLLNIEELLKRYPDELSVGQRQRITAARALV
                     VQPQVIFADEPTGSLDSKSATELLNYLKTMNQKEKATILLVTHDPYTASYCDRILFIK
                     DGVIFSEVVRRGTRREFFEKVIDMQATIGGGGKMNAV"
     gene            16001..18043
                     /locus_tag="IXT20_000150"
     CDS             16001..18043
                     /locus_tag="IXT20_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ4858630.1"
                     /translation="MLYKLAVKSFFRQSRGYLVYFFSLTLSVMIYYSFSAMTYDQSLV
                     RRASQDTSIDGGLSFGGFIITVVLLFFVFSANRFFLNRRQKEIGIYQLFGMNKLQISG
                     IYVLEIMIIGLFACISGILLGIIFSKLFSMILVRMMDMDLTSPFFISIPSVVDTLFAF
                     FIILLAVSVYSIWKIWRYPMIRSFGEKEQADSSTMRFRTRHRLLAVIGLLLLTSGYFG
                     ASHFREITSWMISYHYLDTLVFFFPFLIFFVCVVGTYLFFCYTFRLFLIILSKWKIYY
                     YGINFLIMGNTQVHLLKSWRTNSLITVILGISLMTIGGTASISTILSYQEKVSSPMDY
                     QFDVETEKKIKPILAEEGQKITKEITLHYKVIGSIYSQNLEEPMFQTANLITETQYQA
                     FRKVNPDLPNISLKSPGHTVLLDQMQTLFRNISLYGSSIGLPDNQELKIQQMLPSVLG
                     DETMVYLSPVLIVDDTVFEKAKGVDYQVVNVDVSGGNNEKIDERLSNDLSIKWLNAVY
                     YSYDIVNGSLQGEISTKKLPDDQMKEAQFSQESSRLNFSSSYSKLRAVNRRIGINIFV
                     TLFVGMIVIIATGSILTVRQLAEAEEEKENYHLLTKLGIPQRRIRRMIFEQNALTFFP
                     PMILGITHAVFAINVFTQYIKTADYWLAYLVSGLLILIYLLLYVITSQLYCRIIEE"
     gene            18324..18473
                     /locus_tag="IXT20_000151"
     CDS             18324..18473
                     /locus_tag="IXT20_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala incorporation-associated
                     protein DltX"
                     /protein_id="HAQ4858631.1"
                     /translation="MNKWRSNPKLNYWGKFIIRTIFYSAVLLFLIYLYHYKNIQGGTF
                     IYNEF"
     gene            18484..19998
                     /gene="dltA"
                     /locus_tag="IXT20_000152"
     CDS             18484..19998
                     /gene="dltA"
                     /locus_tag="IXT20_000152"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltA"
                     /protein_id="HAQ4858632.1"
                     /translation="MEIKTIIEAIDDWGINEPDRVAYQADETHTFGELKQASDALAYY
                     LEKKVEGDGPIVVFGNLEFEMIVSFLGVVKSGHAYLPIEEHTPKERILSIFRVAKPSM
                     VISIGDWIEDIPSVPVITKKEFQEIKKIPVGFYPRNSVKGTENFYIIFTSGTTGEPKG
                     VQISHDNLVSFVQWTMEDFGIKPGMHFLAQAPFSFDLSVFSIYPALVSGGMLKPLNKA
                     VVQDFRQLFATLPALKLNVWVSTPSFMDICLMEPTFNGENVPELDMFLFCGEELTKKT
                     AESLLDRFPNARIYNTYGPTEATVAISSIQIDQHVLDSYDRLPIGYVKKDTKVSIVQE
                     GKPVSKGETGEIIIAGPSVSKGYLNNPEKTAAAFFCQNDLASYHTGDAGRLDEYGLLF
                     YEGRMDQQVKLHGYRIELGDIEHYLLQDNRIKQAVVVPKYQGTKVQQLVAFVVLENKT
                     KQPDFQLTKSIKEQLLEVVMDYMIPQKFHYVDLLPQTVNGKIDRKKLTAEVNPE"
     gene            19995..21200
                     /gene="dltB"
                     /locus_tag="IXT20_000153"
     CDS             19995..21200
                     /gene="dltB"
                     /locus_tag="IXT20_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HAQ4858633.1"
                     /translation="MMLPHMIPYAEPFYFFLLAIALVPIVLSLLIWEKRLPVYQSAVT
                     LFFLFVSFGGNYWQQGAALIGYVLWQTILVWGYFVYRQRKNQTGWFFAAVILAIIPLF
                     LTKVTPFFEHGKASLLGFLGISYLTFKSVQMIMEIRDGMIKTYKPFRYIQFLLFFPTI
                     SSGPIDRYRRFEKDQLHPPVRDKYVDLLGKGVQNIFVGFLYKFIIGYYFGQVLLPVVG
                     RIALQHGGISWALVGYMYVYSMYLFFDFAGYSLFAVGTSYMMGYQTPINFNKPFLSWN
                     IKEFWNRWHMTLSFWFRDYVYMRLMFFMMKKKFFKSKIVMSNIGYFALFLLMGIWHGL
                     TWYYIVYGLYHACLICLTDAWIRFKKKHKEQLPSNKFTHGLAVFLTFQAVCFSFLIFS
                     GFLDKLFFS"
     gene            21245..21478
                     /gene="dltC"
                     /locus_tag="IXT20_000154"
     CDS             21245..21478
                     /gene="dltC"
                     /locus_tag="IXT20_000154"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HAQ4858634.1"
                     /translation="MEEQILSILEEITGTDEVRKNRDIDLFDEGLLDSLASVQLLVEL
                     DGELDIQVPVSEFEREDWSTPNKIIQQATALKG"
     gene            21481..22749
                     /gene="dltD"
                     /locus_tag="IXT20_000155"
     CDS             21481..22749
                     /gene="dltD"
                     /locus_tag="IXT20_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HAQ4858635.1"
                     /translation="MKKKLFAIFGPVVLAVGLLFVFFTSSYQVDISNPETIRRASSSM
                     SVNVLKGDAIKNYALSEKQYIPFFGSSELSRISPFHPSVLAEKYQRNYRPFLLGAPGT
                     QSLSQYMMMRSAGDAMKNKKVVFIISPQWFVKNGVKTDYFNTYYSELQTYDWLFSMKK
                     VTPADRYLARRLLTFSKVKENDTLTAILQTIKKGKLPLPESLNQLRSQWNMLKREDEV
                     FSNIGLKDRQQKIDHESKRLPKQYQETELSILANQIGERETTNNPFGLKNDFYTHRIR
                     AHEPELKQSQKNWDYRFSPEFSDFQLVLDQLAKNHNEVLFIIPPVNEKWSDYTGLSQE
                     MLQGFAKKIKFQLNSQGFNRIADFVNQAGTNYFMEDTIHLGWKGWLAADQQIRPFLEE
                     NHITASKYHLDDAFFSKSWQHQIPDKLQLK"
     gene            22890..23972
                     /locus_tag="IXT20_000156"
     CDS             22890..23972
                     /locus_tag="IXT20_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HAQ4858636.1"
                     /translation="MDTKEEQLLIDLTSAKGVPGNEEEVREVFREYAKPFADDIFYDG
                     LGSVIAKHGAGGGPKVFISGHMDEVGFMVTKITEKGFLEFQTLGGWWGQVMLAQQVEI
                     KTREGKIVHGVIGSKPPHVLTPQVRNKPYEIKDMFIDIGASSQEEAKEWGIRPGDMVT
                     PYIEYKRMNGSKYLLAKAWDNRIGTAVSLRVLENLSKEAHPNVLFAGSDVQEEVGLRG
                     ARTSTHLVNPDIAFALDTGTAGDTPGMTPKEADSILGKGPQILIFDASMIPHKKLLNF
                     VIDVAEEMDIPFQYTVISGGGTDAGQMHLTRDGVPSVAITVPVRYLHSHTSVIHEDDY
                     LNTVKLVTELVKRLDSETVEKIRSYE"
     gene            23972..24775
                     /locus_tag="IXT20_000157"
     CDS             23972..24775
                     /locus_tag="IXT20_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HAQ4858637.1"
                     /translation="MNNRIPISEYRKKARVSLENQWGINTWLVFLSIIFTSVIQNFFD
                     GFSPFPEESMQQHLLSFLVDNLLLFAFNYALFYIALVVVRGGRPKSNLLFSVFQRKYF
                     GSILGINLINSVINWLLNSLIFLPMFLISGVSTYMQLLFSSNAVPTGVMPEDFLDISF
                     VLSMIGMIFVSALIMSIVGGLFQFAAWTKFDYPDLSVIQCLKYGWFLLKDRIGTYILL
                     QLSFIGWYLLGAIALFIGLLWVIVYVNVSVAEFYEQARIEKGGPDAYFT"
     gene            24845..25585
                     /locus_tag="IXT20_000158"
     CDS             24845..25585
                     /locus_tag="IXT20_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HAQ4858638.1"
                     /translation="MKLTVLGCLGAYPYKNQGTTGYLLQSDGFNLLIDAGSATLIKLQ
                     EHLDPLDLDAVILSHYHHDHIADLGVLQYYWQLHPKRNPETLLSIYGHTKDSIHFEEL
                     TLENVSKGHPYFEAEELKIGPFLITFMETIHPVVCYAMRIVEETTGKVFVFTGDSGYL
                     ESFADFAKDADLFLADTYLFEGNEHHHAHFTSKEAGELAKSANVKKLVLTHLPQFGDL
                     EQLKNEAQKAAGKGIDIELAAVDKVFNI"
     gene            25596..26600
                     /locus_tag="IXT20_000159"
     CDS             25596..26600
                     /locus_tag="IXT20_000159"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HAQ4858639.1"
                     /translation="MIFVPNENHDPRINLAIEQYLLQEMPLDEPILLFYINEPSIIIG
                     RNQNTLEEINRDYVEENGIHVVRRLSGGGAVYHDFGNLNFSFIMPDDGDSFRDFAKVT
                     QPIIQALHELGVAGAELKGRNDLVIDGMKFSGNAMYATNGRMFAHGTIMFDSDINEVV
                     NALKVKKDKIESKGIKSIRSRVTNIKPFLPEEKQKMTTEEFRQEILLKIFGVESVEEV
                     KTYELTDEDWKKINRISEEYYRNWDWNYGKSPDFNFSRQKRFSIGSIEVHLNVSEGMI
                     KEAKIFGDFFGLGDISDVEKKLVGQKYDKASLSKIVSQIDVKKYFGAIEPEELLTLLY
                     "
     gene            complement(26645..28489)
                     /locus_tag="IXT20_000160"
     CDS             complement(26645..28489)
                     /locus_tag="IXT20_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4858640.1"
                     /translation="MSFFNNIYNKKESFYLRIKWSIIIILSLLFLTGIAYFFYFIFSP
                     ADSNQMKEIKDSWVFYTKDDPDFKFDSKYTRTIPTVDKDETFIMETTLEKPLDEANLL
                     IKGNHQWITVYLGEKILYQRKDYQEESNPGLSLAVIDLPANYIGKKLTLTVSSPYQNY
                     AGLPPKVYLGTTGPLISFIYSQSVPQVLTMIIAVFIAIGTFFYTIYLMYKQKRLEYSL
                     ILLSCFALALGFEAVSEDILSGILFEPFVHSFLSHLFIILTSAFIICYYWSRMFYYKK
                     WYGIFCTFQLLIFIGVLLYATFTPAELPELMPIANIASVISTLVTSLACIGEAYKNNR
                     FYVACTPWIVLVAIIHCLIYIQTALGIYQTTINWSTILYIIILIVIISYNLIDHILNT
                     DKDRRQVDFLKIKNDLLEQHYEQLRQHIQEVNTLRLEFVQEMENLQDLMHTDQTKAKN
                     YVEKILEEAKNFEMLCSFCNHQVTNLILARYQKLAAKRNIQITFQADLPEELPINDDD
                     LAQLLIHALEHSFRETHAIENPLYRKIYLSIHEQEEHFVICCEHSAHYDTNLFSRGIT
                     EELDDQERFDLMMMKDVADRYTGTLTQEKDTFIDRITVQLSRNYPNPV"
     gene            28701..29219
                     /locus_tag="IXT20_000161"
     CDS             28701..29219
                     /locus_tag="IXT20_000161"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HAQ4858641.1"
                     /translation="MPDYKKITFISSITLIIFLILILLVVLVPRIFGLTPYIIEDKEM
                     SPYYAQGNLIYVRPKNTNEILVGDIITYYENSGKKISTRRVIAVENNHEDFYTKADTK
                     TYIEPGVVKKRNIIGSPIFQIPYIGLVLSKTAFAWIQGLFFTIAFCLTGITAWNTFVE
                     LKKKRRRTQKFL"
     gene            29347..30030
                     /locus_tag="IXT20_000162"
     CDS             29347..30030
                     /locus_tag="IXT20_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HAQ4858642.1"
                     /translation="MRNEMMHRPVVKEELLDFMRTQQKKLPGELGKLEEEAHVAEVPV
                     IPHETVVFLKFLLGQLQPERILEIGAAIGFSSSVMATTIGENAHVTTIDRFDVMIEKA
                     KKNYERLGLTDKVTLLEGQAADILPELSGPYDFIFMDSAKSKYIEFLPECLRLLRKGG
                     VLMVDDIFQGGTILLPDEEIPRGKRAIHRKLNEFLRVVMDHPDLTSTILPLGDGVILM
                     TKESETIEL"
     gene            30339..30412
                     /locus_tag="IXT20_000163"
     tRNA            30339..30412
                     /locus_tag="IXT20_000163"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:30373..30375,aa:Arg,seq:tct)
     gene            30571..30930
                     /locus_tag="IXT20_000164"
     CDS             30571..30930
                     /locus_tag="IXT20_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 family protein"
                     /protein_id="HAQ4858643.1"
                     /translation="MTILSRILVTLVAIEFFYIMYIETVRTDSDTTSRVFKMSKEELS
                     RKSVQTLFKNQGVYNGLLGVGLLYGAYLSSASKEITSMLLISIFFVALYGSLTSDRWI
                     LLKQGGLALLALLSIFL"
     gene            31053..32684
                     /locus_tag="IXT20_000165"
     CDS             31053..32684
                     /locus_tag="IXT20_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HAQ4858644.1"
                     /translation="MFKNYVSSLKKEFSGYDLSRFQKDLLAGITVAAVALPLALAFGV
                     SSGADAAAGLITAIIAGIVIGGLSGGFYQISGPTGAMAAILMSIAATKGMQGVLLATF
                     LAGLFLLAAGLFRLGTLTSFIPAPVITGFTSGIAIIIALGQIDNLFGVHSEGTNVIEK
                     LSSYQTIGFPINTASLIMGGLVILGMFIYPKKWAKRMPSSLLAIILATAVMLLFHLPV
                     ATVGKIPQTLISSNRLNMGDFSFSALQQVAVPAISIALLGMIESLLCGASAGRMANKP
                     LDSNQELMAQGIGNLLLPFFGGIPATAAIARTSVAIKSGAQTRLAGMIHAVVLLLSML
                     VFAPVMSQIPMPALAGVLIVTAWRMNEWETIKELFTNKYWSAVLLFILTMICTVIFDL
                     SIAIVIGVIGGCVFFVVKSAAITISLETIDWQRMGLPETEKLNNWTVVYISGPLFFMS
                     SEKLKNKLEELNHHDGVIFSMRGVPSIDLTARAVFDEFQEKAEQRGQTIIYTSLQPEV
                     EKQLSHLWEHTFTHQHQTVAHALTALHAKYTAISH"
     gene            complement(32746..33387)
                     /locus_tag="IXT20_000166"
     CDS             complement(32746..33387)
                     /locus_tag="IXT20_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HAQ4858645.1"
                     /translation="MEYPKELIQEASLRMNGRPVEGFIAGQGPLHPKLMLVGEAPGKT
                     EIDTHVPFSGQAGKELMQALSSTGLTREEVYITSAVRSRPYRVTHRINKRTQQSETVY
                     PNRTPTRSEVFAHAPILDYELMKVQPKLIATLGNIGLQRLLGKEYTVTKNHGQLYTGP
                     VLQLTEQKDAYEWSEKNYRILPLFHPAAIFYNRSLVPVIHQDWQVLASLLQQY"
     gene            33669..33977
                     /gene="rplU"
                     /locus_tag="IXT20_000167"
     CDS             33669..33977
                     /gene="rplU"
                     /locus_tag="IXT20_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HAQ4858646.1"
                     /translation="MYAIIKTGGKQVKVEVGQAIYVEKLDVEAGEKVVFDEVILVGGE
                     STKVGAPTVAGATVEGTVEKHGKQKKVVTFKYKPKKHSHRKQGHRQPYTKVVIDAINA
                     "
     gene            34004..34348
                     /locus_tag="IXT20_000168"
     CDS             34004..34348
                     /locus_tag="IXT20_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HAQ4858647.1"
                     /translation="MIKGTFKRNDAGQIVSFTLTGHADAGPYGSDIVCAGVSALAIST
                     VNGIASLAGFEPIVEMNEEEGGYLYTEVTSGMTQEQNNIAEILLENLLLGLQSIEAEN
                     SEYIQIKTINDK"
     gene            34361..34654
                     /gene="rpmA"
                     /locus_tag="IXT20_000169"
     CDS             34361..34654
                     /gene="rpmA"
                     /locus_tag="IXT20_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HAQ4858648.1"
                     /translation="MLLSMNLQLFAHKKGGGSTSNGRDSESKRLGAKRADGQTVTGGS
                     ILYRQRGTKIYPGVNVGIGGDDTLFAKVDGVVRFERKGRNKKQVSVYPVAQEA"
     gene            35061..37895
                     /locus_tag="IXT20_000170"
     CDS             35061..37895
                     /locus_tag="IXT20_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ4858649.1"
                     /translation="MAKRIDRIYMYVKEKTAHLTKAEYDQGVTTQEIAEVLGIQRTNS
                     SKDLNQLVREGKLIKTDGRPVRYIYQTLVTHVKPVTKHVVSYKETPVSEEKNTLQIDT
                     KDIFAKIIGANGSMKNSVEQAKAAILYPPRGLNCLITGPTGSGKTYFAHAMYHFAKAH
                     HVIDEETELIVFNCADYANNPELLMSHLFGYAKGAFTGADEEKMGIIDQADGGMLFLD
                     EIHRLPPEGQEMIFYFMDHGTYSRLGETTKSHEANVRIVGATTEDPGSSLLETFVRRI
                     PINIKLPAFEKRPANEKVDLVKIMIAHEANRTQRKISLTEDVAKALIGSVTYGNIGQL
                     KSNIQLVCARGFLNHMQSPEISITIDDLTEGIRSGLIQLASNRSAMAELSKILEPKIT
                     VAPNDTIMKIQSDSYELPYNLYDIIGDKAALLKSDGLDQEAINHFISTDINIHLKSFY
                     KDHGFSFNADSKLAEFVDPKIIEVTNQIYVMVKRSLPYEFQQNFIYAMSLHISSFLKK
                     INLGEERHTNDNIREMAFDFPDEYAVAKEVRSYIETYFQVEIPESEDYYLTVLLVSLR
                     ANQASGRIGVVVAAHGNSTASSMVQVVQQLLDAENVRAVDMPLDMDPKTALDRIERNV
                     QEVDEGSGVILLVDMGSLASFNSQIQRDTGIPVRTVDMVTTSLVLETVRKVSVLGTDL
                     DMLHESLKNFRGYAEISSVESVQEAKPIHKKKAVLAICASGEGTAQRIKELIERAVNK
                     RQETELEVLALSIVEVKEELPVIQKEYQIIATTGITDLNIQAPFIPLERFIDQNIEVI
                     LDQLLMESELEETEFISLDEESAKNTCVEFISDNFIFINGSKLIDPMWQFSTQISQTT
                     GIGDEEYGFKINLVMHTAGMIERIIRNEPLTVEENELTNTTNDPLYSQLAASVVLLED
                     QIKVKVPIEEMYYLLRLVHNQLDKKEYTVP"
     gene            38086..38577
                     /locus_tag="IXT20_000171"
     CDS             38086..38577
                     /locus_tag="IXT20_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIB"
                     /protein_id="HAQ4858650.1"
                     /translation="MDIRLARIDDRLIHGQVATVWSKQTGVERIIVISDVVAQDKLRK
                     FLLKEAAPPGITANVITVQKMIQIYHSGLLSRAKVMLLFTNPQDVEAVVRCGVQLKSL
                     NIGGMSFTEGKSMITNFVSVNQQDIDSFRYLNSQEVELEIRKVPADRKILLMELLAKI
                     KKV"
     gene            38680..39669
                     /gene="manX"
                     /locus_tag="IXT20_000172"
     CDS             38680..39669
                     /gene="manX"
                     /locus_tag="IXT20_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HAQ4858651.1"
                     /translation="MVGIILASHGQFAEGILQSGSMIFGEQENVKAVILKPSEGPDDL
                     RAKLEEAVASFDNQDEVLFLVDLWGGTPFNQSNTLFEEHKDKWAIVSGLNLPMLIEAY
                     ASRFSMESAHEIAAHIIETAKDGVKVKPEELEPAEAPKAAVEDAQPKGALPEGTVVGD
                     GKIKYVLARVDSRLLHGQVATAWTKAVQPNRIIVVSDAVSKDDLRKRLIEQAAPPGVK
                     ANVIPISKMIEVAKDPRFGNTKALLLFENPEDVLTAVEGGVDIKELNVGSMAHSVGKV
                     VVSKVLSMGQEDVEAFEKLEQKGVKFDVRKVPNDSRDNMDDILKKAKAELAKA"
     gene            39702..40505
                     /locus_tag="IXT20_000173"
     CDS             39702..40505
                     /locus_tag="IXT20_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HAQ4858652.1"
                     /translation="MSIISIILVVLVAFLAGMEGILDEFQFHQPLVACTLIGLVTGNL
                     EAGIVLGGTLQMIALGWANIGAAVAPDAALASVASAIILVLGGQGVKGVPSAIAIAVP
                     LAVAGLFLTMIVRTIAVPIVHLMDAAAEEGNIRKVEMWHIIAVCLQGVRIAVPAAALL
                     FIPAQTVQSFLESMPAWLTDGMAIGGGMVVAVGYALVINMMATKEVWPFFVIGFVVAA
                     ISQLTLIALGALGVALALIYLNLSKMGGSSNNGGGSNSGDPLGDILNDY"
     gene            40526..41440
                     /locus_tag="IXT20_000174"
     CDS             40526..41440
                     /locus_tag="IXT20_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HAQ4858653.1"
                     /translation="MAEEKIKLSKKDRLAVAWRSTFIQGSWNYERMQNGGWAFSMIPA
                     IKKLYKTKEERSAALKRHLEFFNTHPYIASPILGVTLALEEERANGAPVDDVAIQGVK
                     VGMMGPLAGVGDPVFWFTVRPMLGALGASLAMGGNILGPIIFFLGWNIIRWAFMWYTQ
                     EFGYKAGSKITDDLSGGLLQDVTKGASILGMFVLAALVQRWVSIKFLPVVSTVKLDKG
                     AYIEWDKLPSGGEGIKSAFEQVNNGLALSPEKVTTLQDNLDQLIPGLAALGLTLLCMW
                     LLKKKVSPIVIILGLFVVGVVGHVIGLL"
     gene            41523..41897
                     /locus_tag="IXT20_000175"
     CDS             41523..41897
                     /locus_tag="IXT20_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF956 family protein"
                     /protein_id="HAQ4858654.1"
                     /translation="MVQSINTKVDLVIDATSFTGLTDYGQIMIGDNGFEFYHARDRRK
                     YIQIPWEEVDYVLASVMLKGKWIPRYAIQTKKNGTYTFSSKEPKKVLRAIREYVDEDR
                     MVQSLSFFDVVKRGIRSMFKRK"
     gene            42036..42281
                     /locus_tag="IXT20_000176"
     CDS             42036..42281
                     /locus_tag="IXT20_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAQ4858655.1"
                     /translation="MSWLWTLIVGAVIGAIAGAITSKGNSMGCVTNIIAGLVGSSIGQ
                     AIFGEWGPKAAGMALIPSILGAVILVAVVSFFFGKRS"
     gene            42453..43517
                     /locus_tag="IXT20_000177"
     CDS             42453..43517
                     /locus_tag="IXT20_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HAQ4858656.1"
                     /translation="MMLRVEKLRKKMQEENLDSFLVTSPYNLRYLTNFTGTTGLAVIT
                     LEKAFFITDFRYTEQAAAQAQGFEIIKNVGPIFDEVANLVRKEELHSLAFEETTVSFL
                     EYSVLEEIIDAELVPVSQMIEELREVKDEEEIAIIEKACHIADMAYDHILKMIQPGMT
                     EIEVANQLDFYMRSLGASGVSFDTIVASGLRSAMPHGVASKKIIEQGDLITLDFGCYY
                     EGYVSDMTRTFAIGDPGEKLKEIYQIVLDAQLAVIDAAKPGMTGVQLDAVARDYISKH
                     GYGEAFGHSTGHGIGLEIHEGPNVSSRAEKQFVPGNVITDEPGIYLPGIGGVRIEDDL
                     LITEDGNRVLTRSPKELIIL"
     gene            43763..44188
                     /locus_tag="IXT20_000178"
     CDS             43763..44188
                     /locus_tag="IXT20_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HAQ4858657.1"
                     /translation="MADEKNLILNANQDLGEIVIAPEVIEVIIGIAASKVEGVYGMRG
                     TFASNVTELLGRAAHGKGVYLANEEDGLKVDLYCYLEYGVSVPKVAMEMQERVSQQVL
                     FMTDIDLSEVNIHVVAVVPEKLEQPTIEELFEDEEEENE"
     gene            44181..44630
                     /gene="nusB"
                     /locus_tag="IXT20_000179"
     CDS             44181..44630
                     /gene="nusB"
                     /locus_tag="IXT20_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HAQ4858658.1"
                     /translation="MNKELSRHEIREMALQALFPLDFNADLTKEDAIFNAIELDHRDM
                     INEDESEFVPVYLDTLVGGVCAKKDELDKVIEKHLKNNWHINRISKMDLVILRIAIFE
                     MTYVTDVPAAVALNEAIELAKTFSDDRSRKFVNGVLSNVLKELEAGA"
     gene            44727..45581
                     /locus_tag="IXT20_000180"
     CDS             44727..45581
                     /locus_tag="IXT20_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HAQ4858659.1"
                     /translation="MAELINGKELAEKMQAEIAEKIQKLKDNGIHPGLVVLLVGENPA
                     SQIYVRNKERVAKEIGIHSLVERYPETISEDELLAEIEKYNQDPSFHGILVQLPLPKH
                     IDEEKILLAIRPEKDVDGFHPMNLGRLLAGKPDKIPCTPYGIMKMFEAYSIDPAGKRA
                     VVIGRSNIVGKPMAQLLLMADATVTVAHSKTENLAELAQEADILVVAIGRGHFVTKEF
                     VKPGAVVIDVGMNRDANGKLIGDVKFDEVEPIAGWITPVPKGVGPMTITMLMYQTVKS
                     AEAFGAGE"
     gene            45582..46919
                     /locus_tag="IXT20_000181"
     CDS             45582..46919
                     /locus_tag="IXT20_000181"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HAQ4858660.1"
                     /translation="MAQEYLTVTTLTKYLKRKFDADPYLERVYLTGEISNFRLRANAH
                     QYFSLKDDHAKISAIMFKGAFQKLRFQPKEGMKVLVVGRISLYEAGGSYQIYVEHMEP
                     DGIGALYQELEERKEKLMKEGLFQGPKKMLPRFPKRIAVLTSPSGAVIRDIITTVKRR
                     YPIVQLVLFPTVVQGNQAADNVVRNIQRVEEMGNFDTMIIGRGGGSIEDLWPFNEERV
                     ARAIYQANTPVISSVGHETDTTIADLVADVRAATPTAAAELAVPVLSEELMRIQERQA
                     RLQQAFVRQIQRKQERFERARRSYVFRQPERLYEGQTIKLDQLKQRLYQATQQIYHAK
                     EKQTIALSHQLEQVAPLYRVKAARQETAYLEKRLEEKIQQYMKQQQQRFAQAVQSLDL
                     LSPLKIMGRGYSYTTLNDQVVKSVTEIHQGDLLTIHYQDGIVKGQVQEIDKGE"
     gene            46924..47148
                     /locus_tag="IXT20_000182"
     CDS             46924..47148
                     /locus_tag="IXT20_000182"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HAQ4858661.1"
                     /translation="MENPTFEESLQELEKIVMQLEQGDVPLESALDSFKRGMELSKHC
                     QDTLAKAEETLTKMMTESNEEVDFDGNEES"
     gene            47150..48040
                     /locus_tag="IXT20_000183"
     CDS             47150..48040
                     /locus_tag="IXT20_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HAQ4858662.1"
                     /translation="MRLTDFSAIHLPAVEKEILSFLDEHTTDRGLYDAMSYSVKAGGK
                     RIRPLLLLTAVASFDEPIDVPVYQVAAALEMVHTYSLIHDDLPAMDNDDLRRGKPTNH
                     KVFGEALAILAGDGLLTGAFQLISMAHLGNSPKLLLLQQLAVCAGSQGMVAGQAADIE
                     GESKKLSLEELAFIHERKTGHLIRYALLAGGILAKQPEEILLLLQRLAEHLGLAFQIR
                     DDLLDVIGTTKTLGKTAGKDERMEKNTYPRLLGLEKTREALEIELLSANKIIDKLEED
                     VFSFDGGLMRQMIKQFDVEK"
     gene            48049..48867
                     /locus_tag="IXT20_000184"
     CDS             48049..48867
                     /locus_tag="IXT20_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HAQ4858663.1"
                     /translation="MEKERVDVLAVKQGLFETREQAKRSVMAGLIYNEKNERFDKPGE
                     KIPVSSELRVKGKKLPYVSRGGLKLEKALKAFDLSVEGKTLLDIGASTGGFTDAALQN
                     GAKMSYALDVGYNQLAWKIRQDPRVVVMERVNFRYAKPEDFTLGIPEVAVIDVSFISL
                     KLMLPPLHAILKEEGEVIALIKPQFEAGREAVGKNGIVRDPQTHQKVLEDILSFAAQG
                     GYDVLELSYSPITGGEGNIEFLAHLRKVPESGTINSAINMAEVVSNAHEQFDHK"
     gene            48974..49423
                     /locus_tag="IXT20_000185"
     CDS             48974..49423
                     /locus_tag="IXT20_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HAQ4858664.1"
                     /translation="MRKKDRHRLITRLLSEQDIRKQEEFVEILKSKGISVTQATISRD
                     IKELKLIKVPASDGGYRYSIPAETNEDVNTKLEKLLKDAFVAVDQMEKFVILKTLPGN
                     ASAAANLIDKRYKKELFSIINDDDNVLMITRTEDQAVLLKKDFLHYL"
     gene            49441..51123
                     /gene="recN"
                     /locus_tag="IXT20_000186"
     CDS             49441..51123
                     /gene="recN"
                     /locus_tag="IXT20_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HAQ4858665.1"
                     /translation="MLQEISITNFAIIPELRLSFHEGMTALTGETGAGKSIIIDALGL
                     LAGGRGSSDYIRQGAEKCVLEGLFELPKQEGFSELMVELGIETDEDNLIVRRDMSLTG
                     KNVCRVNGHIITLANLRRIGSYLVDIQGQNEHQELLQPESHLALLDRFGDVAFQQKKK
                     SYQQEYISYRELEKRVRKVQQNEKSYVQRIDMLHFQQEEIAAADLQVGEEEKLKEERE
                     KLSNYQKIADGLAAGYGALTNNEQNSVDGVGLAVSELQGIAHLDVEYEAIYENIQSAY
                     YLLQDAIGDMSRQIDLLELDESRLEEVTQRLELIRQLKRKYGESIESILAYYDEITEE
                     LASSDFSEGQLDKMKEELEQKELLLQQQAADLHEARKKIAKELEKSILHELKSLYMEN
                     TEFEVRFLKEENRQLNHDGFDQIEFYITTNPGEPLKPLVKVASGGELSRMLLALKTIF
                     SSEQGVTSIIFDEVDTGVSGRVAQAIADKISQISKYSQVLCITHLPQVAAVADYQYYI
                     VKAVIDGRTQTSVSELKTKEREEEIARMLAGSEITKLTLEHAKELLKLAKRT"
     gene            complement(51178..51294)
                     /locus_tag="IXT20_000187"
     CDS             complement(51178..51294)
                     /locus_tag="IXT20_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4044 domain-containing protein"
                     /protein_id="HAQ4858666.1"
                     /translation="MREKKQTSTFAKVTKFVIWTMLILTIGSVVLTAVMSVM"
     gene            complement(51367..52323)
                     /locus_tag="IXT20_000188"
     CDS             complement(51367..52323)
                     /locus_tag="IXT20_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HAQ4858667.1"
                     /translation="MIKYFTLDNEKLRNTGKETDQTIWYCVERPNQEEIHQLTHQFEI
                     PSDYITSILDDAENSRTEEFNQSKLTKPALLLLQYPFAKTSPSGYFQVDTYPLSIILT
                     PKKKLITITNHEPLFLKKVFSQTFPKSDISPNLNIFFQFIWQVTLSYNSYLATLQSQC
                     DTLEAKLQVSTENKQLYQIMDIQKSLVYFREATSANLDALKELAQNKLFQENHALKNH
                     LRDVLVETEQAMTTSRIQLKLVEQMNQTFSAIVSNNLNNIMKILTSLTIILTIPTIVG
                     SIYGMNIKLPIAERDDAFIWLVLLTLFICIVTTYYLKKGKFL"
     gene            52494..52862
                     /locus_tag="IXT20_000189"
     CDS             52494..52862
                     /locus_tag="IXT20_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3397 domain-containing protein"
                     /protein_id="HAQ4858668.1"
                     /translation="MGTFTPLILFWYIYPVIVLFGCQFLVKLFSLNRRFKIKAPDLAI
                     PFLWGGMHALSRNTGTISILPFFFISVLLMGICIAVFQAYYYEEIIYPRYFKMTWRLV
                     FLLTIVLYAVLIIVNILSYL"
     gene            53032..53463
                     /gene="mraZ"
                     /locus_tag="IXT20_000190"
     CDS             53032..53463
                     /gene="mraZ"
                     /locus_tag="IXT20_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010754126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HAQ4858669.1"
                     /translation="MLMGEFQHNIDAKGRLIVPSKLREELGEKFVLTRGLDGCLFGYP
                     MSEWENLEAKLNEMPLAKKDARTFVRFFYSAATECELDKQGRINIPSTLRNYAALTKG
                     CVVIGVSNRIEIWDETRWQDFSAAAEENFDEIAESMIDFGL"
     gene            53484..54443
                     /gene="rsmH"
                     /locus_tag="IXT20_000191"
     CDS             53484..54443
                     /gene="rsmH"
                     /locus_tag="IXT20_000191"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HAQ4858670.1"
                     /translation="MTETFQHYTVMLKETVDGLNIKPDGVYVDCTLGGAGHSEYLLSQ
                     LSSKGHLYAFDQDQKAIDHAKLRLASYVEQGMVTFIKANFRELEERLSEHVDKVDGIL
                     YDLGVSSPQLDEAERGFSYHQDAPLDMRMDQSAPLSAYHVINEYSYHELVKIFFRYGE
                     EKFSKQIAREIERVRKTKPIQTTGELVEIIKQVIPAPARRKGGHPAKRIFQAVRIAVN
                     DELGAEEASLEQAIRLLKVNGRISVITFHSLEDRIVKSMFKEYSTVQDLPPGLPVIPD
                     EFQPELKVITRKPILPSEEELTENNRSRSAKLRIAEKIRMKEE"
     gene            54448..54849
                     /gene="ftsL"
                     /locus_tag="IXT20_000192"
     CDS             54448..54849
                     /gene="ftsL"
                     /locus_tag="IXT20_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HAQ4858671.1"
                     /translation="MAELKKMQDYEYDLPELVDQPEQQMPDSSSNQEIILPQSPAKRL
                     KQISALEKIAVASVAIAIVALCVLTVMLRTNISGAEKEITSIQTEINDKKQEKTSLEQ
                     EKNELSRTERIKQIAEEKGLSINDDNLRKVK"
     gene            54850..57042
                     /locus_tag="IXT20_000193"
     CDS             54850..57042
                     /locus_tag="IXT20_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HAQ4858672.1"
                     /translation="MSLKNKIRRFMEKKNLNPMNNRKKVGIILFATSIGLFFLFAVRF
                     SYIVIGGHVAGTSLAEKTKQLYQGSEVVKAKRGTIYDRNGVALAEDASSYSIKAILSK
                     TYTSGDKKLYVEEKNFDKIAEILHKNLSIDKKDALNILEDGAKKELYQVEFGSYGKNI
                     SQETKQNIEADMKKEGVAGLYFVDHQARMYPNGVFSSHFIGYAVPDKDENGLVGKLGL
                     ESAYNDILSGKDGKIIYQKDNFQNPLPGTVAEEEKAVDGQDIYTTLDSRLQSYLETLM
                     DQVNEEYQPEELTAVLMKAKTGEILAMGQRPTFNPETMEGLTGKDAIWRNFLVQDSYE
                     PGSTMKVFTTAAAIEEGEFNENETFQSGKIQVEDATINDHDFGEKGVLTMRQALSWSS
                     NVGMVILEQRLGGRWYNYLQKLGFGQSTHSGLDDEVNGALPTLNIVDRAMSAYGQAVG
                     VTNFQMMKAFTSIANNGTMIQPRYISKVVDPQTGEERTTQTEVLGQPFSKETTEKVRE
                     YMRDVVESENYGSAYGVYSVPGYNVSAKTGTAQIASDTGGYQTGDTAYLYSIVEMVPS
                     EDPDYVLYLTMKHPKTYDRMALAKIANPLMKRAMDFKETEEDSDTETKTEKVSVADYR
                     NLEADVAAADAQKSGLQPVVIGNGKKVQKQSTANGDQLISGEKLILYTGGDKLMPDVT
                     GWSKADIMKLGKILGIEVSFDGDGYCVKQELAPYEKITEDKLSFTLEE"
     gene            57068..58030
                     /locus_tag="IXT20_000194"
     CDS             57068..58030
                     /locus_tag="IXT20_000194"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HAQ4858673.1"
                     /translation="MEWTQALIPIVSSCAMTIAAMPLFIGYFQMKKQGQAIREEGPKW
                     HNSKAGTPTMGGLVFLIGSILTGIWVGAWQKQLTPTLFILLFVLALYGVIGFLDDFIK
                     IFKKRNMGLNSKQKLIGQILGGIIFYLVYRSEGYPGVLNFFGLDLPLGIIYGIFALFW
                     LVGFSNAVNLTDGIDGLVAGLGSISFAAYGLIAWHQHQYDVLIICLSVLGGLLGFFVY
                     NRKPAKIFMGDVGSLALGGLLAAISIMLNQEWTLLLIGLIYVMETASVMLQVTSFKLT
                     GKRIFKMSPIHHHFEMCGWSEWKIDTVFWLISIVTSLITLWIVW"
     gene            58044..59405
                     /locus_tag="IXT20_000195"
     CDS             58044..59405
                     /locus_tag="IXT20_000195"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HAQ4858674.1"
                     /translation="MKKISTYENKKVLVLGLAKSGVSAAKLLHELGALVTVNDGKPFD
                     ENPEAQELLSLGIKVITGSHPIELLDEEFSLMVKNPGIPYSHPLVAKAQEMGIPVITE
                     VELAYEVAECPIIGITGTNGKTTTTTMTGLLLNAGADQGIARLAGNIGYPASGVAQEA
                     KSEDKIVMELSSFQLMGITDFRPHIAVITNIYEAHIDYHGTRKEYVKAKWNLQKNMTE
                     KDYLILNWNQSELQELAQRTKARVLPFSTKEVLEDGVYADDYSIYYKKEKIMEISELG
                     VPGKHNVENALAAISVAKLYGISNEAIRETLHFFHGVPHRTQYVGEIQGRKFYNDSKA
                     TNILATKMALSGFETSKVVLLAGGLDRGNTFDELIPSLKGIKAMVVFGQTKEKLMDAG
                     KKAGIETIVTADSVEQAVPLALENSTDGDVVLLSPANASWDQYPNFETRGNRFMEAVN
                     RLK"
     gene            59431..60519
                     /gene="murG"
                     /locus_tag="IXT20_000196"
     CDS             59431..60519
                     /gene="murG"
                     /locus_tag="IXT20_000196"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HAQ4858675.1"
                     /translation="MKILVTGGGTGGHIYPALAFVNYVKTQEPNAEFMYVGAKRGLEN
                     KIVPDTGMPFHTLEIQGFKRKISMHNIKTVRLFLKSIRQAKKILSDFQPDIVIGTGGY
                     VSGAVVYAAAKMGIPTVIHEQNSVPGVTNKFLSRYVDRIALSFKDAASFFPENKAVLV
                     GNPRAQEVADTKKSDILKTFDLDPEKKTVLIFGGSQGALKINQAVSVFLSEYIPSSYQ
                     VLYASGDRYYEEILNKINPEIQNISVQPYIKNMAEVMASSDLLVGRAGATSIAEFTAL
                     GLPAVLVPSPYVTNDHQTKNAMSLVHAGAAKMIADNELTGESLSQTVNEIMGDEELQK
                     QMCRASKEQGIPDASKRLYDLVKQIIKK"
     gene            60534..61754
                     /locus_tag="IXT20_000197"
     CDS             60534..61754
                     /locus_tag="IXT20_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HAQ4858676.1"
                     /translation="MSKKKEPKKLSHPSSDENNLTPWQKANRKYLAEQEMKNQEPKKE
                     DITLDTPLSDSEEEKTVQEEQQEESQSEKKIEFFEEIKQKKNGGPYNGSFLNRLPNLK
                     SQRHKVLFRRLALIITVLGIPLIFLIYYVSPYSKLQAVAVSGNKTVNSQEIISDTKLS
                     LGENVWGQYFHRSTYIDRLKKAQPRIETANIHFKGMNEFDLDVTEYKEIALIAKNNQY
                     YPVIENGTVLDEKVANPTKNLPILEEFKDSAKIKELTKQYNQLSSELQKAISEIKYTP
                     RASNKNLIQLNMNDGNQVIVNINNLANQMKYYSQVAKDMDEKGVIDMEVGIFSHPYGS
                     TQETEESGQSEETTESEENADQTNESSEEAETQKAQTPGSSSTNGSSDSNDSSETSQI
                     EDNPSTTPSSLNEN"
     gene            61910..63235
                     /gene="ftsA"
                     /locus_tag="IXT20_000198"
     CDS             61910..63235
                     /gene="ftsA"
                     /locus_tag="IXT20_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HAQ4858677.1"
                     /translation="MAKTGMYVGLDIGTTSVKVVVAEYIDSQMNIIGVGNAKSEGINR
                     GIIVDIDKTVQAIQRAVRQAEEKAGIQIKGVSVGLPANMLEVENCQGMIAVNGDSKEI
                     TDEDVRNVASAALVRSIPPERQIVSILPQDFTVDGFEGIKDPRGMIGVRLEMYGLLFT
                     GPKTIIHNIRKCVENAGLIVNEMVITPLALTESILSDGEKDFGTIVIDMGGGQTTTAV
                     MHDKQLKFTNLDQEGGEFVTKDISIVLNTSFNNAEALKINYGDAYPERTSPDEEFPVD
                     VIGQSEPVKVDERYLSEIISARMEQIFNKAKDALDQIEALELPGGVVLTGGAASLPGV
                     VDLAQEIFGVNVKLYVPNHMGLRNPVFTNVISIVDYSANLSEVYQLAKIAVTGETSAA
                     RQMVVEQETTNTYESYEAPEETIYDEPEPKESGENVKNKIKGFFTNIFD"
     gene            63258..64499
                     /gene="ftsZ"
                     /locus_tag="IXT20_000199"
     CDS             63258..64499
                     /gene="ftsZ"
                     /locus_tag="IXT20_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HAQ4858678.1"
                     /translation="MEFSIDNNINDGAVIKVIGVGGGGGNAVNRMIEENVKGVEFITA
                     NTDVQALKNSKAETVIQLGPKYTRGLGAGSQPEVGQKAAEESEQSLREALDGADMIFI
                     TAGMGGGTGTGAAPIVAGIAKELGALTVGVVTRPFTFEGPKRGRFAAEGIARLKENVD
                     TLLIISNNRLLEVVDKKTPMLEAFREADNVLRQGVQGISDLITAPGYVNLDFADVKTV
                     MENQGTALMGIGVASGEDRVVEATKKAISSPLLETSIDGAEQVLLNITGGLDMTLFEA
                     QDASDIVANAATGDVNIILGTSINEEMGDEIRVTVIATGIDESKKERKSSRPARQAQM
                     QSPAQKTVLDMDQAKPTSSEEENSFGDWDIRREQNVRPRVDDSNFDNIEKKEFDTFNR
                     EETKSHGDDELNTPPFFRRKK"
     gene            64516..65193
                     /locus_tag="IXT20_000200"
     CDS             64516..65193
                     /locus_tag="IXT20_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HAQ4858679.1"
                     /translation="MIADNLQKIKQEIQDSCALVHRDPKEVTLVAVTKSVESAQAEEL
                     VRAGVMNCAENRVDKLLEKKQDLFDFPEIKWHLIGNLQRRKVKLIVNEIDYFHALDSL
                     RLAEEIQKRLEKELSCFIEVNVSGEESKHGISPDELLPFVESLAACTKIKVVGLMTMA
                     PKEASDEQIHKVFGTLKELRDLVQAKGYAHAPCSELSMGMSQDFQIAIEEGATFIRVG
                     TALFKDE"
     gene            65207..65806
                     /locus_tag="IXT20_000201"
     CDS             65207..65806
                     /locus_tag="IXT20_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HAQ4858680.1"
                     /translation="MPLLNRGAIANFFGLADEEEYEYQDYPETTKKAVQPTKKNTTQN
                     QSTHSVSAANTHEKQKTATSRPVTHESIQPAEKKKVIELHQSPTKTVAKERTASRTST
                     QPKANLAGKITVMEPRAYSEAMAIAKRIIAGEATLVNFHLVEEKQARRIVDFLTGTVY
                     ALDGDIKRVGDEIFLCTPTGMEIDSSTAQSFADANLFDL"
     gene            65821..66108
                     /locus_tag="IXT20_000202"
     CDS             65821..66108
                     /locus_tag="IXT20_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HAQ4858681.1"
                     /translation="MYTIALLLHLLQRAAYFYSIILMVYALLSWFPGGYQSGLGRLLA
                     KICEPYISLFDRLPLRIGPLDFTILGAILILNLAVQGLAQIVIWITVHLVY"
     gene            66157..66939
                     /locus_tag="IXT20_000203"
     CDS             66157..66939
                     /locus_tag="IXT20_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HAQ4858682.1"
                     /translation="MDANVYQHFRADERTFIDSVGDWIEQVQGQYAPYLTEFLDPRQS
                     YILETLIRQSSDLRFMFYGGYEQAERKRCLIFPDYYEPVEEDFETALYEIHYPSKFAS
                     LSHGKILGTLVGTGIKRSYFGDIISDGDRWQVFIANEVDHFVAAQVTKIGKVAVRLEP
                     IKYTEMIMPKDSWTQERGTVSSMRLDSVISEIYNISRQRSKQLIEAGKVKVNWTENTR
                     PDFVLELLDIISVRGYGRVQIQELEGKTKKEKYRVLFGVLRK"
     gene            66960..67667
                     /locus_tag="IXT20_000204"
     CDS             66960..67667
                     /locus_tag="IXT20_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HAQ4858683.1"
                     /translation="MALTPLDIQNKTFPTKMRGYNQDEVDDFLDLVVRDYEELTQRNR
                     ELEKAVKHSEEKLEYFNELKDALNQSIIVAQDTADKVKTSASKESEVIVTSAQNKADE
                     LVANAEKRAHQLTTDAEEKARKILTDATEKARQLATETEDLKKKTRVFHQRISLMLES
                     QLEQVKSPEWDEILQPFSSYVTDSHEVIKEVLAKQLDNENDTDVNSDTGVIEAPMTAF
                     DTQAIDISIVPGENENE"
     gene            67929..70727
                     /gene="ileS"
                     /locus_tag="IXT20_000205"
     CDS             67929..70727
                     /gene="ileS"
                     /locus_tag="IXT20_000205"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HAQ4858684.1"
                     /translation="MKMKETLQLGKTKFPMRGNLPNREGEWQKEWEENRIYQKRQELN
                     EGKPSFVLHDGPPYANGNIHLGHSLNKISKDIIIRSKSMSGYRAPYVPGWDTHGLPIE
                     QVLANKGVKRKEMSMAEYLEKCREYALSQVDKQRADFKRLGVAGDWEHPYVTLDPSYE
                     AAQVRVFGKMAEKGYIYKGLKPIYWSPSSESSLAEAEIEYKDVKSPSIFVAFNVKDGK
                     DILDEDTAFVIWTTTPWTLPANQGIAVNPKFTYVLVEADGRKFVVAKDLLETVQSEIG
                     WENVTVLKEFAGQEMEYMTASHPFYERESLVILGDHVTLDAGTGLVHTAPGHGEDDYI
                     AGSKYKLSVVSPVDSKGLFTEEAPGFEGIFYDKANPMITDLLKEKGALLKLDFFTHSY
                     PHDWRTKKPVIYRATPQWFASIDKFRQNILDEVEKVEWVIPWGKTRLYNMIRDRGDWV
                     ISRQRAWGVPLPIFYAENGEAIITPETIEHVAKLFAEHGSIIWFEKEAKELLPEGFTH
                     PGSPNGEFTKEKDIMDVWFDSGSSHEAVLRQRPELSFPADMYLEGSDQYRGWFNSSIT
                     TSVAINGVAPYKSVISQGFALDGEGRKMSKSLGNIIAPEKVIKQFGADILRLWVSSVD
                     YEADVRVSMDILSQVAEVYRKIRNTMRFLLANTEDFDPKKDTVSYNDLRSVDKYMTVR
                     LNQVIKEIREEGYDKYNFMHIYRTVMNFLTVDLSSFYLDFAKDVVYIEAEDDYQRRCM
                     QTVFYQTAVALTKLLTPIIPHTAEEIWSFLKEEEEYVQLSEFPGYEEFANQEELMDTW
                     TAFMDFRDNVLKALEEARNSKLIGKSLEAKLTVYPKQQVREMLKALDADIAQLLIVSP
                     DYFEVMAADEQVPDNAMVFDDVAITVEKADGETCDRCRQVRKDVGVDEKLPHLCGRCA
                     KIVEDFYPEAVAEGFEEK"
     gene            complement(70776..72302)
                     /locus_tag="IXT20_000206"
     CDS             complement(70776..72302)
                     /locus_tag="IXT20_000206"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HAQ4858685.1"
                     /translation="MSNEKNVLFTIFGGTGDLAQRKLYPSLFRLYRKGNLGEHFAVIG
                     TARRPWSDEHYREVVKETIRALNPSDEEATTFASHFYYQSHDVNDSTHYQTLKELSDR
                     LDGHYQLNGNRVYYLAMAPQFFGTIVSHLKSQNIMTKNGFNRLIIEKPFGSDYQSAFE
                     LNQQIREVFPEQDIFRIDHYLGKEMIQNISAIRFANNIFEAQWNNRYIDNIQITFGEN
                     LGVEDRGGYYDHSGALKDMVQNHILQVVALLAMEPPVAFSEKEIRTEKIKALKAIRIY
                     NEEEVLENFVRGQYAQGELDGVQFKGYREEDKVDAQSETETFVAGKFTIDNFRWSGVP
                     FYVRTGKRLTEKGTRINIVFKQVPVNVFKTSVDEPCDDTTLPPNVLTIYIQPTEGFSL
                     SLNGKEVGQGFETEPIKLEFRNSAEMVENSPEAYEKLLLDALNGDGTNFSHWEEVAQS
                     WHIVDVIRKAWDQTQPDFPNYKAGSMGPKAAFELLEKDGFEWIWQPDNWYRERGKLND
                     "
     gene            complement(72317..72964)
                     /locus_tag="IXT20_000207"
     CDS             complement(72317..72964)
                     /locus_tag="IXT20_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005881023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HAQ4858686.1"
                     /translation="MTPNREDYLKIILELGGDTTKVNNKQIVSSLDVSAASVSEMISK
                     LVKEELVEHSPYQGVQLTASGLQKASSLIRKHRLWEVFLVEHLDYSWNEVHDDAEVLE
                     HVTSEHLADHLENYLNHPEHCPHGGSIPKKEEVVHEERRQTLESYPVGTKVRIARVLD
                     EKELLDYLVTIDLNINEEYEIVDIAAYEGPITIQNDRKKIPVSFKAASTIFVDKI"
     gene            complement(73148..73477)
                     /locus_tag="IXT20_000208"
     CDS             complement(73148..73477)
                     /locus_tag="IXT20_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4828 domain-containing protein"
                     /protein_id="HAQ4858687.1"
                     /translation="MIYFLGSLITVLSSFIYLKKKKQAEKSEGLSKYQGTWFFSDSQQ
                     KQQLQITSDYTFLINGQSLGTSVVEIDEYRLVVRDQYGFYLIFSYPEQTLYDESADTS
                     YKLLSEK"
     gene            73654..74382
                     /gene="sfsA"
                     /locus_tag="IXT20_000209"
     CDS             73654..74382
                     /gene="sfsA"
                     /locus_tag="IXT20_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HAQ4858688.1"
                     /translation="MKYQQVEIAFFIDRPNRFIAHCMKKDGEIIIAHVKNTGRGKEVL
                     IPGAEVAIVFAPGPKRKTAYDLIAVKKKGDWFNIDSQLPNRLAIDGILDGTILLPGLT
                     GPFTVYKREVTYLHSKFDIYLETVVGQKAFVEVKGMTLENKAIGAFPDAPTLRGLKHI
                     GELTYAAQDGYAAYVLFIAQFEHLHLATIHEEMQPALADMVRHAQQSGVQILAYNCQV
                     SADEVTVKQAIPFDVDIPFEDPIQ"
     gene            74398..75411
                     /locus_tag="IXT20_000210"
     CDS             74398..75411
                     /locus_tag="IXT20_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAQ4858689.1"
                     /translation="MINLGIIGTNWITHQFVQAALSTKKYELTAVYSRHLEKAQEFGS
                     HYEGDIEFATDLKTFFNITHMDTIYIASPNSLHFSQAKQAILAGKNVIVEKPAFSTPK
                     EMDEIIHLANEHNVYFFEAARNIHESGFKKIADYLPLKDEIIGANFSYMKYSSRYDQV
                     LDGEEPNIFSTHFSGGALMDLGVYLVYAAVAWFGMPKEVHYFPRKISTGVDGLGWGIL
                     RYENFDVSIQPGKIGDSYLPSEIYFDSGTLVMNGVNAIEQAQFHDRAHKEIIDLAVTA
                     AENPMADEAEDFANVMAHPKDPAWGLLYEEWIELARNVNQVIYDLRKNGGIKFDADNE
                     EDF"
     gene            75411..76688
                     /locus_tag="IXT20_000211"
     CDS             75411..76688
                     /locus_tag="IXT20_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HAQ4858690.1"
                     /translation="MNEFVQVFPENWQKKYEDKGFISPSLIQRAVFQPLSNKQSIVAV
                     SPTGSGKTLAYLWPLLLNVEPGEASALVIFASSQELAIQVADVAREWGKDKELKVQSL
                     VGGANVKRQIEGLKEKPEILIGTPGRILELMKAKKIKAHQVKTMVFDEADQLFDAGNS
                     QIIDQILHQAPTEYQLAFFSATADRSLESIEKITGKTFETIDVTAEDDSRKGLRHYFL
                     RVPIRKKDDYLRRLTHIEDFHGLIFFNQLTELGVMEEKLLYRGVPVGSLASDQNKLLR
                     KAALEQFKKKMVRALLTTDVAARGLDITGLPYVVNAEVPLSEEAYLHRSGRTGRMGNE
                     GIVITLVQDNNLRDLKRLLRPSNITLEEIFLYGGKLQTEQPTKEDAAPAVNKKYAKTF
                     SGKEQNPEYNKEKIRKKVKNKSKKERNKGARRK"
     gene            76751..79453
                     /locus_tag="IXT20_000212"
     CDS             76751..79453
                     /locus_tag="IXT20_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ4858691.1"
                     /translation="MKERQEEILQLLTENQQGLTASDVAERLTIDRSNASRYLSELYK
                     AHHIVKTAGRPVVYSLPTEKSKSDEVHVDSSTQVTFETLVGENDSLKVSIQQAKAAIL
                     YPPRGLHTIIFGETGTGKSMFAECMYHFAIDSEMLSADAPFVSFNCADYAQNPQLLFG
                     HIFGIKKGAYTGAAQDSPGLIAKADGGILFLDEIHRLPPEGQEMLFTFIDKGIYRPLG
                     ESAQVHEASVQIIGATTESSESFLSTFNRRIPMAITLPNLASRSLDERYEIISLFIKQ
                     EANRLNQRIDIEKEAILAFMLYDAEGNIGQVKRDLKLVCAKSFLHYRTHHEKKLIIRK
                     EELSLQVQKGLLKIKEIPERLDRFMDSKSQYLTFEPGFADVVWSQDPERNMQVYNDIE
                     EKVTSLSETGVENIDLETLISKDVDAYFQTYVKELTKSSIPKDLLPDEIWRLTNQLYD
                     VAEKRLDRIYNEKARFAFALHLQSTLDRVREGHMIVHPDLNNVRKNFKREFQVAIDLS
                     TIIEEEEQVEIPFDEIGFISMFLSINLGESEPVKENKVDVLILMHGRQTASSMLEMAQ
                     ELLGTKVGKAFNMPLVMEVQTMYEEVLQYVKNYQEHLTNGLLLLTDMGSLNTFSDLIY
                     EETGIRTKAITMTSTMIVIEAIRMASVGRSLEDIYQNIQLSFESIVRDQFKAEKSETL
                     QRKKAVIVTCFTGEGVAAKLYQRIMPVINQNKVELIQMQFLERESFKKHIDALLEEYE
                     IKAIAGTVDVDYQNIPFFSAYDVFDDEKLNILKRIADDEIPTAKIVDSLAGSLSDVFS
                     IDELIRSLQKTVQQIQTDLSIIVEPGVDTGIVIHLAFMLDGLIKQESHRQFEKLSIFS
                     KIYRLEMDIVRANLMALERKYQVKIPEDDIAFLTQMFLENKIKK"
     gene            79605..79922
                     /locus_tag="IXT20_000213"
     CDS             79605..79922
                     /locus_tag="IXT20_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ4858692.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEEKGMEADIFAVSASDA
                     DNNLESKNVDVLLLGPQVRFMKAQFEQKLAPKGIPLDVINMQDYGMMNGEKVLAQAEN
                     LMK"
     gene            79952..80272
                     /locus_tag="IXT20_000214"
     CDS             79952..80272
                     /locus_tag="IXT20_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HAQ4858693.1"
                     /translation="MEDQQNLEAIMGLIMFGGNAKSDAMEAIAAAKIGDFELADRKIA
                     DAEESLIQAHHAQTDMLTQEAQGNHMQVTLLTVHSQDHLMTSIAFTDLAKEIIELYRR
                     VYSE"
     gene            80380..81840
                     /locus_tag="IXT20_000215"
     CDS             80380..81840
                     /locus_tag="IXT20_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ4858694.1"
                     /translation="MNGLSSWMEKYILPVAAKIGAQKHLVALRDAFIGTLPATMAGSV
                     AVMINAIIRDLPQQFNSTYADSLAADKGIFAVLNVIIGINGFVWNGTLAIAGLIFAFS
                     WGYNLARAYGVNDLAGGIVGLATLIQGISFGPALSAALDVNVPSNVADAINSSEIGAT
                     IADGTLSVGLWGWLKLDHLNGNAYFTVMIMGAISVIIFCKLMLANITIKMPDSVPPAV
                     SKAFAAILPATIALYVIAIINYIVGRVADGQLIIDLVQKYIAEPFLGLSQGLGAVLIV
                     TVFVQVFWFFGIHGPNVLAPVLEGIWGQAQLINIDIFQKGYNGKTGTPAVLDAIEDGK
                     AYMWVRGSFDAFAWFGGSGGTIVLLIAILIFSKRADYLTVAKLSIGPGIFNINEPVMF
                     GLPIVLNAIMFIPFLIAPVVATAIGWTATYLGLVAPVSQQVTWVVPPFLLSFLATGAD
                     WRAPIVTLVCMVVTFLIWAPFVISANKMDPAAGEQQ"
     gene            81908..82129
                     /locus_tag="IXT20_000216"
     CDS             81908..82129
                     /locus_tag="IXT20_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4858695.1"
                     /translation="MITIASAMQQDEIKELLEGYNEEGMTFTFKEKQGIKLFFETSAE
                     DPEVAAKKAKELIKAQPWGTVLYFQSTAV"
     gene            82160..82342
                     /locus_tag="IXT20_000217"
     CDS             82160..82342
                     /locus_tag="IXT20_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3188 domain-containing protein"
                     /protein_id="HAQ4858696.1"
                     /translation="MIKNGLFLCSIGSLIVLYSLNPHSQRYDLYSMVTGLFLTGLGIY
                     FFMKGKKREEAKEKNN"
     gene            82342..82755
                     /locus_tag="IXT20_000218"
     CDS             82342..82755
                     /locus_tag="IXT20_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3284 domain-containing protein"
                     /protein_id="HAQ4858697.1"
                     /translation="MEITKKLNIPASFFYDKVMDSVLFDVRKATGKSVTRKQLKNLEY
                     VKQFSQNNRARIKIEEVIENQSYKFRTSTTKNEFVVHYQIKPLDDSTCEVHYTEKMES
                     YGMLQKLNDMLLGTILMYFKRRRFKKMLDMIEATY"
     gene            82878..84059
                     /locus_tag="IXT20_000219"
     CDS             82878..84059
                     /locus_tag="IXT20_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ4858698.1"
                     /translation="MTNKVRELGVSSIEEMFDLATYAFNGADTPERRERFRTLAENSW
                     NYGFFDEEDRLTSQVMATPFPVNFYGQEYLMAGIGYVASYPEARGQGGINQIMEKILE
                     DCRDRKVSLSYLAPFSYPFYRRYGYEQSFDKISYQLNSRDVPFIKKKSGKIKRMDFED
                     AKAAIRRIYEQMPENQRAGLKREDWWYTYKFKQKGNYQFALHFDDNEEATGYIVYQLA
                     TSSFIIAEWGFLDHDAFCSLVRFVSSHNGAFETFEYSCGYGGNDQNYLLENPFASVMI
                     TPYMMARIVDISLFLENYPFKKRQAAFALQIEEDHYAKWNEGIVEVTIENGKNHLSKV
                     EQTKLPIVRGSIQHITQLLLGYRKVEELVFQERIQVTKEYEETLSSILPQQQPILNDY
                     F"
     gene            84448..84521
                     /locus_tag="IXT20_000220"
     tRNA            84448..84521
                     /locus_tag="IXT20_000220"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:84482..84484,aa:Arg,seq:cct)
     gene            complement(84558..84758)
                     /locus_tag="IXT20_000221"
     CDS             complement(84558..84758)
                     /locus_tag="IXT20_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAQ4858699.1"
                     /translation="MKINYTVDLVSFSLVINFSLFSLRLFIDGLRHTHASILLYQGVN
                     ILSVSKRLGHSSLETTMSTYTK"
     gene            complement(85063..86295)
                     /locus_tag="IXT20_000222"
     CDS             complement(85063..86295)
                     /locus_tag="IXT20_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HAQ4858700.1"
                     /translation="MLSDFNELLKKYARLIAETGVATEKGHTVVLQISVEQAPLARLI
                     TQEAYKLGAAEVIVQWTDDQIQREFLLHAATDRIENVPQSKIDQADEWLEKGASRISV
                     VSADPDAFAGVDSHRVASYQSANGKALMNLRKATQANKVSWTVVAAAGKQWAAKVFPE
                     LPEEKQVEALWDQIFKTTRVYEEDPVLAWKKHDEKLAKKAEELNREQFSALHYTAPGT
                     DIIIGLPKNHLWEGAGSYNARGEKFMANMPTEEVFTAPDSHRVDGYISSTKPLSYAGT
                     IISGMKFTFKDGKVVDFSAEQGEDVLAKLLDTDEGARRLGEVALVPDPSPISQSGIIF
                     FNTLFDENASNHLALGSAYAFSVKGGTEMSDEELAEAGLNRSQTHVDFMVGSDKMDID
                     GIREDGSTVPIFRNGDWA"
     gene            86549..87118
                     /locus_tag="IXT20_000223"
     CDS             86549..87118
                     /locus_tag="IXT20_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAQ4858701.1"
                     /translation="MARKKTITREQILAAAYEVAATDGFTRFTARNIANKMNCSTQPI
                     YLEFKNMDDLRQALFEQIYDHLQYEVFTVEHTGNTIIDLAFNYIHFARREKKLYRSLY
                     LEEYGGGKEMQNFSYTYFVNTVKRDPAYVNLSEEQIESLYNGTWIIATGIAALMSSSI
                     IHPTDEQIEKMIQDSIDAILKFKEPIEID"
     gene            complement(87296..87736)
                     /locus_tag="IXT20_000224"
     CDS             complement(87296..87736)
                     /locus_tag="IXT20_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HAQ4858702.1"
                     /translation="MALAKIVYASMTGNTEEIADIVAEAFENLDIEVEINECTQVDAD
                     EFEEADICVVATYTYDDGDLPDEIVDFYEDLQELDLLGKIYGVCGSGDTFYDEFCKSV
                     DDFDAAFAKTGASKGAENVKVDLNAEEEDIENLEAFAKELVAKI"
     gene            87894..88658
                     /gene="map"
                     /locus_tag="IXT20_000225"
     CDS             87894..88658
                     /gene="map"
                     /locus_tag="IXT20_000225"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008377375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HAQ4858703.1"
                     /translation="MITLKSAREIEMMAESGALLADVHKNLRDFIKPGITSWDIEVFV
                     RNYIESHGGIAAQIGFEGYEYATCISINDEICHGFPRKKPLKNGDLVKVDMCIDLKGG
                     ISDSCWAYVVGESTPEIDRLMEVTKKALYIGIEQAQVGNRIGDIGHAIQTYVEGENLA
                     IVRDFIGHGVGPTIHEEPAVPHYGEAGKGLRLKEGMVITIEPMVNTGTWKMKMDPNGW
                     TAYTRDGGLSCQYEHTLAITKDGPRILTSQGEEGTY"
     gene            88690..89613
                     /locus_tag="IXT20_000226"
     CDS             88690..89613
                     /locus_tag="IXT20_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HAQ4858704.1"
                     /translation="MKVWNKIKQNENLMRFIETTQKRITDSEMSTTSVVVAYYLLLSL
                     FPLIIAFGNILPFLHIDQETVLTYLREVIPETVYQFIGPAIKDLLTQSSGGLLSVSAL
                     AALWSASQSINALQIAMNKAYGVENRKNFLIVRFFSLIVIVLFMIAISGVTIVLGLGQ
                     LILDAIQPIVQIPVNFIDQFQTLKWPITLVALFVIMFLIYLIVPNAQLKLKTIIPGTI
                     FATVGWMLLSQVFSIYARYFATRISGYQIIGSFIVLMLWLNFAATIIILGGIINAVVQ
                     EYVTGHQIKKRRDPSRKWFNKLKTKFSHKKK"
     gene            89689..90828
                     /locus_tag="IXT20_000227"
     CDS             89689..90828
                     /locus_tag="IXT20_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl/decaprenyl-phosphate
                     alpha-N-acetylglucosaminyl 1-phosphate transferase"
                     /protein_id="HAQ4858705.1"
                     /translation="MLVSFSMVIRFFLTILLSLILTPVFKIISVQTGMVDKPNERRIN
                     KVPMPSAGGLPIFISFVISTLFLFRKIIPQSYIVPILIASLVIIVTGVLDDKYELTPK
                     QKSIGILIASLIIYFVADIRIDSFTLPFIGYIQLGWLSFPVTIFWIFGITNAVNLIDG
                     LDGLAAGISLIGLITIGIIGYFFLHASTVYIPVVIFCLVASIIGFFPYNFYPAKIYLG
                     DTGALFLGFMMAVLSLQGLKNVTFVSSISLLIVMGVPVTDTFFAIIRRKANRVSFSTA
                     DKKHLHHRLISLGFTHKGAVLTIYAMALMFSFTAMVMNYTGRIGTIILIIAMLFAAIL
                     LFELIGLINEKHQFILKTLRFLGNKEYRTQVKQKYLKKWNRHIDE"
     gene            90821..91621
                     /locus_tag="IXT20_000228"
     CDS             90821..91621
                     /locus_tag="IXT20_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAQ4858706.1"
                     /translation="MNNKLNQPILISIVTANSDKIFRTLDNFISTVKDETAVKIRIFD
                     NNSLPEYKKRLTEYRKYPYIDIYFHDENSGFGYGHNHNLIASDSRYGIICNPDILVDE
                     ETVTALVLLLKEHPECAMLAPKILNEDGTTQHLIREQLAVFDYVLRFVPFKWVKKKFD
                     KRLAVFECRGLPTDANSYVRMISGSFMVADIDKFKEIKGFDERFFMYFEDNDLCLSFE
                     KAGYKLLYTPFYAVIHFYGKGAHRNAKLFRVFLTSMMKFFNKWGWRFF"
     gene            91621..92448
                     /locus_tag="IXT20_000229"
     CDS             91621..92448
                     /locus_tag="IXT20_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAQ4858707.1"
                     /translation="MKPEILVVVVLYQQLFSQSPAYGPLSKALSEKQIQLIIYDNSPQ
                     RQQNELFEKENIVYHHDPQNPGLAVAYNFALSQADEGTRCLVTLDQDTRLVDDYFELL
                     RKVTFTDECVAAVPMIFSGSRQVSPLYSDRYINRTAQAVEVEGPTAERIMAINSGVAW
                     SVKFLKKIGGFNPAFSLDFLDHWLFWKVDQLEKTVEILPVRMEHDLSVLDYEKVSSNR
                     YRSILQAENRFYHEYDQRHLSSHRRQLLLRTAKQFLTVKNRHIWRMTLRSYVKNWKV"
     gene            92426..93160
                     /locus_tag="IXT20_000230"
     CDS             92426..93160
                     /locus_tag="IXT20_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAQ4858708.1"
                     /translation="MLRIGRCEFVRSVCIATFNGGRYLREQLDSILSQLSAEDEVVIS
                     DDGSVDDTWDILTEYAEKDKRIQLFSGPRQGLIANFGYAIQQTKGDLIFLADQDDVWL
                     ETKVKKITDYFEQNPDQLVVVSDLIIVDSELQEIHDSYFSFRNSKEGWLNNVIRSHYI
                     GAGMAFRSSLKPDILPIPSEVPMHDMWIGLLGGKKTGFLREPLTLYRRHDLNASEIET
                     HSKLRQKISWRVHLITALIKRKWFKR"
     gene            93260..94126
                     /gene="rfbA"
                     /locus_tag="IXT20_000231"
     CDS             93260..94126
                     /gene="rfbA"
                     /locus_tag="IXT20_000231"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HAQ4858709.1"
                     /translation="MKGIILAGGSGTRLYPLTKATSKQLMPIYDKPMIYYPMSTLMLA
                     GIKEILIISTPQDTPRFKELFGNGNDLGLQIEYAVQESPDGLAQAFIIGEEFIGDDSV
                     CLVLGDNIYYGGGLSKMLQRAASKDTGATVFGYHVNDPERFGVVEFDENMQALSIEEK
                     PAQPKSNYAVTGLYFYDNEVISIAKGIKPSERGELEITDVNKAYLDKGKLSVELMGRG
                     FAWLDTGTHESLLEASTFIETIEKRQNLKVACLEEIAYRMGYIDKEQLLALAQPLKKN
                     QYGQYLLNLAAE"
     gene            94140..94712
                     /gene="rfbC"
                     /locus_tag="IXT20_000232"
     CDS             94140..94712
                     /gene="rfbC"
                     /locus_tag="IXT20_000232"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010771301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HAQ4858710.1"
                     /translation="MKVTDTKLTDVKIVEMDVFGDHRGFFTESYSKAKFAEHGLKYDF
                     VQDNHSLSAEAGVLRGLHFQKGEAAQTKLIRVVTGAVLDVVVDIRKGSPTYGQWDGFI
                     LSEHNHRQLLVPKGYAHGFVTLTPNVNFLYKCDNYYNAEADGGIAFDDPDLAIDWPID
                     PSRAIMSEKDHRHPTLKEFEAENPFVYGEI"
     gene            94734..95762
                     /gene="rfbB"
                     /locus_tag="IXT20_000233"
     CDS             94734..95762
                     /gene="rfbB"
                     /locus_tag="IXT20_000233"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HAQ4858711.1"
                     /translation="MKNIIVTGGAGFIGSNFVHYVVNNHPEVHVTVLDKLTYAGNKEN
                     LAGLPSDRVELVVGDVADAELVDRLVKEADAVVHYAAESHNDNSLKDPFPFVQTNIIG
                     TYTLLEACRKYDVRYHHVSTDEVYGDLPLREDLPGHGEGEGEKFTAETPYNPSSPYSS
                     TKAGSDLLVKAWVRSFGLRATISNCSNNYGPYQHIEKFIPRQVTNILSGIRPKLYGEG
                     KNVRDWIHTNDHSSAVWLILTKGRIGETYLIGADGEEDNKTVMELILEMMGQPKDAYD
                     HVNDRAGHDLRYAIDSTKLREELGWKPEFTNFRDGLADTIKWYEEHEDWWKKEKEAVE
                     AAYAKNGQ"
     gene            95860..96711
                     /gene="rfbD"
                     /locus_tag="IXT20_000234"
     CDS             95860..96711
                     /gene="rfbD"
                     /locus_tag="IXT20_000234"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HAQ4858712.1"
                     /translation="MFLLTGGNGQLGTELRHLLDEKNLQYVSTDAKDLDITDAEKTMA
                     YITELKPDVIFHCAAYTAVDKAEEEAKELDEKINVDGTRNVALAAKAVNAQFVYISTD
                     YVFDGKNKNVEYEVDDQTNPLNEYGRTKLLGEKAVEEILEDYYIIRTSWVFGIYGHNF
                     VFTMQRLAETRDKLTVVNDQFGRPTWTRTLAEFMVYVIEEKAPFGIYHLSNENSCSWY
                     EFAKEILKDTDVEVAPVTSEEFPQKATRPQYSVMSLKKTEELGFVIPTWQEALAQMLE
                     AAKKMNK"
     gene            96745..98778
                     /locus_tag="IXT20_000235"
     CDS             96745..98778
                     /locus_tag="IXT20_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4858713.1"
                     /translation="MEKQTRSARRKEKPKANQADSNLVLDLFLKLRYLIGVLVIVVIV
                     TFNLNGSSLGVWDKYVSQRDDGKKTDVIFGQNREVRSDEWLVQTPFYLSQAEEDFPLV
                     NEDYSLNGQNMIIAYNSPVKDITVIGKPFNWGFFFLGRDRGLSFYWAMKLVVMVLLGF
                     EVLMILTKRKKALSLIGAFALTFSPAVQWWFMQHVGDLIFFTLGLMTAFYHYFYNHDK
                     KWLRTLMMLLVVIFGIGFILVIYPAHQVMLAYLLIFYFVGLLIYYSKKVSWDLLDVVL
                     IGGAVLFIGGVMVHFWLTSKDALLASLNTLYPGKRVSTGGKWTIGEFFSFLTNWKIPF
                     EDINYSNNSEVALFYHFFPTVFLASPFVLFGKKDSEQKLLGRILMIFCLFSIFWITVG
                     LPKWLAEITLLSYVPPLRAILTFSFAACLLSIWFIAYIWREDVIPIWYKFILLAVNAW
                     SYFYAITHSKMDNYFSDFETIAVAVLGTVILAFALFGWRRLFYLAFAALVIVSGFFVN
                     PVVEGTGAIFEKTLAKEIQKIDQQDPGKVWLSEDELYNFTPTLGVKSFNTVRFYPDIA
                     AWKKIDPKEEYEKYYNRYAHTRAFIAKEDTKFILDRPDMFSVTLNFEDAEKLGIDYVI
                     SKRPLTDYNQLYEAQFTQLYGPDKDGYMIFKVEYPDYPDVPQTQTQENQLVVQ"
     gene            98822..100102
                     /locus_tag="IXT20_000236"
     CDS             98822..100102
                     /locus_tag="IXT20_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EpaQ family protein"
                     /protein_id="HAQ4858714.1"
                     /translation="MEIIQAIVQKFVNKMSVGFLLLLLTLVFFVWTAGLGFPATLFIY
                     NNSSLLIAFSVAILLILNVYKAKGVDFLYLGVALALFIFFSYFRELRNESLFGDLLFP
                     LIILFCLVIKWIEWTKWDRGIYLIVFSVFLGITLYRVFTEIQVPEGQSIWAPGNSLSD
                     IWINTNIIGSSLMTLALLISGFASSFERWYVRIIGAVAVVAAFLGIWVCQSRGALVAL
                     IVFAILDLLPKSLMRVIRAPFIAYTVTILLALPISYLAAVSEKVNLFTGREDIWHKFY
                     QTLGEKSEQILLGMKTFIFQRGNQFLGNHNSYNSILNIYGLIGFGIAALLLILFIGRL
                     TLKADLSNGQMTFIWAFFAVMMQSFMEDTLTSYPWVPIIYILLAMAVHRYDEPEVAEE
                     FYVMEEAYDDMTYDDTSYEEQPDSAHSSRIQRYH"
     gene            100312..101118
                     /locus_tag="IXT20_000237"
     CDS             100312..101118
                     /locus_tag="IXT20_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ4858715.1"
                     /translation="MFKDLYLFMKDIYQNRKLLLQFSINDFKSRYAGSFLGILWAFIN
                     PLFTVLIYWLVFGVGLKAQMIDAKYPFIVYLVTGMIPWFFFSDAFASTTVVFREYTYL
                     VKKVVFNIRILPTTKILSNLYTHLFFILIGIAVALINGIYPSAMSLQLIYYLFCMCAF
                     LTGLTWLTASIQPFLPDIMQLITIVLQLIMWTLPILWSPNQFDSGLVSILKLNPLYYV
                     VQGYRESFLSEAWFWEHGTYTLYFWVFTLVMFVIGSAVFRRLKPHFSDVL"
     gene            101130..102350
                     /locus_tag="IXT20_000238"
     CDS             101130..102350
                     /locus_tag="IXT20_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ4858716.1"
                     /translation="MTEYAIRLKDITKSYNMYAKPSDRFREALNPFKKSYHDVFYALK
                     NVNVEIKKGEMIGFVGENGSGKSTMLKIITGVLTPTSGTMEIEGKISALLELGSGFNP
                     EYSGYENIYLNGMVLGFSREEVDEMVDDIIEFADIGDHIYQPVKTYSSGMFVRLAFAV
                     AINVNPDILIVDEALAVGDLEFQLKCMEKFTEIKNSGKTILFVSHDISSIRRFCDRSY
                     WLKNGEVVEYGDTMEVTTNYENFLKKKSVKTVDRNKNQVERFAAPDIVDVTKAELLDK
                     NKNPVDMLEQGEKLYVKVTYEVKDDTIKNPVLGIALRTVDNFYVCGLNTLLDGIKIPW
                     KKGENIFYLEYEKLGLLAGEYYFDVAVFEENATVPLVYKTKYMNLFVSGSYIGEGIVV
                     LDHKWEEGTHSNEI"
     gene            102340..103926
                     /locus_tag="IXT20_000239"
     CDS             102340..103926
                     /locus_tag="IXT20_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HAQ4858717.1"
                     /translation="MKYDFEMDLDEQSSVGKIAAQIKPGSKVLEFGPGNGRLTKHLIG
                     AKQCEVSIVELDKELFDFVSEFAQDGFYGDIESFEWANYYAGQTFDYVLFADVLEHLV
                     DPGKTLKKVREFLNEEGEILITFPNLAHNSVMIDLFNNQLPWASYGLLDETHNSFYTH
                     DGFQKVFEKAGLFINIEDYLYLAVGDTELKSTYEELPEAVRYDFKMRPFGEVYQYFFS
                     LMKHPVVQTSIAQPQNSNYVKVLEVTQQTKQDETIQQIPFNNFTGENETLSFPVSEAT
                     ERMVFRFAQQPSFIEFSAEAAGEKLIFIDSNAVVKTVNDCYLFDGKEIPEFALTDISG
                     KEVTIHCHYRFIGELTPTMKELLETIRPMAEVTKQLSEKNDELERENQHLLEENTRLD
                     HTLKTTTNRYCTLLESDEWTIKHRLGRRKKKTSKKIQEKELSICIDEKIWDAETKILK
                     IIGWGIANSDRQPLSYKLSADQSPFFEAIPVSRDEVNQAEQLAKGTEAGFELRILCEQ
                     ERSFLVEAVAQNGQSWIIEM"
     gene            103965..106103
                     /locus_tag="IXT20_000240"
     CDS             103965..106103
                     /locus_tag="IXT20_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAQ4858718.1"
                     /translation="MQKKEIAVFIDQITRERATGDLLIVGWAIDEVTKEIPTIKVEKE
                     NVIAEATHVVRLDINHLYNLDVKTQSGFKIRLSGKMRGKAILDFQTAKHQNGIAVKLN
                     GKYPYDDGIESSWERKKRLLKKGINYARTHGVKKTIRRVKLELKPGSIDYAEWISRHE
                     KPDLKEQRKEAQGFAYRPLISILMPVYNVEIKWLEKCIDSVLDQTYDHWELCISDDAS
                     TDPAIRKCLESYQAKDDRIKVVFRQENGHISLATNSALEMAEGEFIALLDNDDELPPF
                     ALYEVAKVLNIHPELDLIYSDEDKIDADGNRFDPHFKADWSPDTLMGNNYISHLGVYR
                     TSIVKKLGGFRKGYEGSQDYDLVLRVTEQIPADHIYHIDRVLYHWRTIPGSTASNGEA
                     KSYIYDSGVKALTDALSRRNIKGSVRPGRISGFYEIAYDVLQEDLVSVIIPTKNGYED
                     LKTCVDSIIEKTSYPNYEIIIADNGSTDPKMQELFAEYKHQLKDRFIVELIDIPFNYS
                     RINNLAAEKANGKYFLFLNNDTEVIEPDWMTAMVSYAQFDRIGCVGAKLFYPDDTTQH
                     AGVLLGIGGVAGHALNNYDRTHCGYFGRLVIDVNYLAVTAACMMVKAADFNAVNGFDE
                     TLEVAFNDVDLCLKVYELGRYNVYAHQAELYHFESKSRGYEDTPEKQRRFAGEIKKMQ
                     DKWPSYIAHDPFYNDNLTKEGIGDFSLRPD"
     gene            106391..107782
                     /locus_tag="IXT20_000241"
     CDS             106391..107782
                     /locus_tag="IXT20_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HAQ4858719.1"
                     /translation="MNKNGEWNAARRIFIIIMDVLVLNVSIYLSFLLKFQGEVPARNL
                     ETFQHSAVFISIIFIALNILLGAYVFYSRVISDIVFITVIGQVLIALGIMVVTFAGRW
                     FAFPRGVIVLSFILGTILLSVYRIVVYKLYLRVSRDKKVLIVGLEKDVAPAIQNFQSK
                     KNNKHKVEAVVIDKYYENTKKLIDDIDIVYLASKIPEDEKINIYQLVTKKEKKLFLNT
                     TFENLTMINPNIMNFEDESIIEASGFKIQPEYELFKRLFDIIVSLILLILASPFMLIT
                     AILVKTTSPGPIIYKQVRITKNQKEFSIYKFRSMSVTAEAKSGPVLAKSNDARVTPVG
                     KFIRAVRFDELPQIFNVLKGDMSIVGPRPERPFFVDQFNEENPYYYLRHNVRAGITGY
                     AQVYGKYVSDYNSKLRFDLLYIKKYSLLLDLKILLQTIKILFDKMSSQGLDEDENIRS
                     LAEIDFSQEKIYR"
     gene            107841..108650
                     /locus_tag="IXT20_000242"
     CDS             107841..108650
                     /locus_tag="IXT20_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAQ4858720.1"
                     /translation="MLLSLYDKEKPEYLKEALTSIFEQTVMPKEIVLVYDGPINEKLQ
                     VVVTYFQQKDPHLFTVVKLEKNQGLGIALAIGLEHTKNRLVARMDTDDIMEPQRLEKQ
                     LEIFQKDPEIAIVGSNIEEFTGTLSNVIGRRIVPEYNDEIRVFSKRRNPFNHMTVMFD
                     KNAILEVGNYRPLIGFEDYYLWVRLLKAGYKGYNIQEILVHARAGADMYARRGGSKYL
                     LPGIKGRYRVWKDGLGSLKDFLIVICGHLVISLLPNKIRGKVYESKLRKTN"
     gene            108622..109611
                     /locus_tag="IXT20_000243"
     CDS             108622..109611
                     /locus_tag="IXT20_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAQ4858721.1"
                     /translation="MNQNLEKQTKLSIIIPFYNAKNTLKRAVDSVLTQGYPNIEILLI
                     DDGSTDGSSDIGDEIANLESKVACYHLENAGPSVARNCGIKEATGNLVGFLDSDDYFL
                     PDCFNNVLKDYHRNYPDIIAFGLSKGKNIENANLFVPEKKIGCSSEEAIEQMFRSKAV
                     DFYVWNKFFKRELFNHIVFPENKLYEDMVPMYEAFRAASKIDILPFSGIFYYQNNDSI
                     VYQSFNSKQYDNILQRKILVDEVKKDYPRLINLANARLVDGYLSTGFKITGKNQNQKE
                     KQKYYNQSKREIKSIYSKIRSNNEISIAKKLALFLYLINPKTYFILYKKVLKK"
     gene            109725..110720
                     /locus_tag="IXT20_000244"
     CDS             109725..110720
                     /locus_tag="IXT20_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAQ4858722.1"
                     /translation="MHKISVIVPAYNVEDYLENCLESISNQTHSNIEIIVVNDGSPDR
                     SGEIAEEYKKKDMRLKVIHQVNGGLSAARNSGLDSATGDFVCFIDSDDWIEPDYLELL
                     YFGTQTYGADISVLKIKKISSLEQLKEHTIAERKWEIFDREQAMKNLFINNKIGYSAN
                     NKLFNRNLFDNIRFPLGKLMEDKATTYLLIDHSEKVAVNSSAKYHYYQSPKSILRGSF
                     NPRIFDSFEVHEDILIFIDKFYPELSINVRTRYVYEAIRMMMTMIKNNYTNKEDYLRS
                     INILQRFEKEVKIDPDFSPKLKKLVKFLLINKNLPYYMSSSKIASKFLIKIESVR"
     gene            110724..111866
                     /locus_tag="IXT20_000245"
     CDS             110724..111866
                     /locus_tag="IXT20_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid biosynthesis protein F"
                     /protein_id="HAQ4858723.1"
                     /translation="MKNIKKFFSRNIFPVFIKILPIQKNLIVFSSFGGRNYSGNPRVI
                     SEKIHEKKLNYRIIWLIKKEAIITDLPDYIKRIDSDSFLAAFYLYTAKYWIDDSRKTI
                     KYAKRKKQLYFQTWHGTPLKKIEFDAKDKLPKRYLSYAKKDSESITYLLSGNRYSSKK
                     YVSAFNIVPSKILEVGTPRNDELASSKEQVFDLKKKQINVLFAPTFRNNIEDNGITQL
                     EWLGVDNLREFFKKQQQELNFMTKFHPNVHSKLATDSKSIEYMKKHQITLINDGIPLE
                     MLFEEIDVLITDYSSIFFDFALTKKPIILFNYDEKEYSLERGFYIQLSELPIISVQDS
                     YGLVDVFKNQQEKLLKSSQELLDYIGNYETGASTEKVLELIERDIK"
     gene            111863..113290
                     /locus_tag="IXT20_000246"
     CDS             111863..113290
                     /locus_tag="IXT20_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HAQ4858724.1"
                     /translation="MKKTVKDIFYNAIYQVFLIVLPLLTIPILSRRIGSTGLGIYGYV
                     FSISQFLMTVIAVGMNPFRIRNIAKSRKDKKALSLQFWNIYFIQFLIGLSVSFLYIAY
                     IYFFDVKYKNLYLIQLIFIVGLTLDISWFFQGIEEFSKVVIRNTVIKLSSVLLIIIFV
                     STKDDLWIYVFITSITNFLGSLVFWLSIHGKVGKPIFNSNVFKQLWKPAFIILTPQLF
                     MQVYTTLDKTIVGYFVDPTELSYYDQSQKIARIILAVLTSITIVMLPKISKAQAEGKS
                     ENILVYTKKSFDYTIIIALILFSVVFINTKEFVPWFFGENFKPMTINMLISCLIIVVS
                     PIGGVFANQFAIAIEKDKEYAYPMIIGAIVSIIGNVAFVPTYGAVAATIVLVVVELLV
                     FFFRVFFVRKEIKISYFFGKNVILFFIVNLTLTTWAYYFLPQLFSSSFLDMAIKSIMI
                     FIIYLPFVYFLFPDIRNEIRKITRK"
BASE COUNT        36904 a        19085 c        23697 g        33631 t
ORIGIN      
        1 agttggtagg agaacaccgt ttaccaagag gttgtgaaaa agctgttctg catcaagtaa
       61 taagaagagc caagtaaaaa cagtctctta tgtttttaac ttggctggac ttatcaccgt
      121 agatgtaagc ttttgaacgc cgtttatttg caacaaaagg ggctgtgaca cgatttttgt
      181 cacagccctt tttttgcgaa cattcattta gcaattttta gaaacttctg ccgccaccgc
      241 caaaagtccc gccaccgcta gaatgggtag tacttccgcc accgccgctt ccaggaggtg
      301 ggcttttggg gattcttctt gtcgtgacaa aagaatttgt cagacgatcg gttttttcag
      361 ttaatttgat actggatttt tctcggaatg gatatttgta gatgcctgat ttcaattgat
      421 aacgcgaaac agtcacaaaa taaaagacaa gactaaggat aagagccaaa acaacagcaa
      481 tgatgatttc tactgtcgtc aaaaccttat agcgtgtaat tttgccagtt tcttcgtcta
      541 cacgataatg tcctcctgga actccgtctt ccacatatgt tgctgattta gacaggtaag
      601 cttttgcagc attgtaatag gcagcatttg tcatctgatc ataaacatca tctaagatcg
      661 aatcgattct gctatcagtc aaataatcga tcatattacc agaagtcgaa atgtagattt
      721 cacgctgtcc catatctaat agcaatactg agccattttg atcatttccg actgcgtctc
      781 ttaagtaatt atcagcaaag tctcttggtt cttctgaatt agatgtagtc gttacgatca
      841 agactcgtcc tttgattttt tcattcagct tttctgcctg ttgttccaaa gattggattt
      901 gatctgttgt gaataaccca gcctcatcat ttaccgtagc tgtatctgca tgagtagcca
      961 ccggccagcc aacacttact agcaaaagaa agaataaaaa aaattttttc atcatatcaa
     1021 gtaacctccg atcagaaaga ggatcgctag cccagcgaaa atcccaagag cggtcagtcc
     1081 caattttttc tggctgatcg gcaggacacc gctgaccttt cctgtttgtc cattcattgc
     1141 gtagtagtag acttttttac ttgagtcatt gcttcgataa gtaacaagcc ataccggcag
     1201 taatacgtaa ttatttactt cgtttgtgat tgaagcagaa gttcgcacac cggtcaatgt
     1261 tgtatatcca tttgcggtat cttttaacag cttctctgaa tagtctttta attcagattg
     1321 aatctgtgat ttgattgcct catattcgat atctcttttt tctgcttgga acccggctaa
     1381 atactggctt ttaaaatcaa ttgctttatc taacgggaaa ggttgcacgc cttcgaccat
     1441 cttttgttgc gtattttttg ataaggcatt ttttactagt tctttaaaag aaagcttgcc
     1501 tgctcttgaa acagcaaatt gcttcgtttc tgtgtattcg atgtcaccta tccgccatat
     1561 gcggataact gtaccattcg cctgcatggc accatctact tcagcatctg tagcccaata
     1621 agggaaatac acacctgtca acttatcgat ttgatcctta ttgaaaaaag ctttgggaac
     1681 aaaccatttt ttcttggtcc aagcgagaaa tctgttgatt gcttcttctt tctcgacagc
     1741 aaaaggaagt actttatttg gcaagaattc tccgcttaaa cgtcccgata gtacgacggg
     1801 attatggcag tagtaacaat aagtcgcagc agttgtcgca tcagtcacga tctctgcacc
     1861 acagttggga caaagaaaaa gatctatcgc tgcggtatta tctgcagatt cgtccgctgt
     1921 atctgaaaaa ccgcctgcct cgtcaaccgc tttgcgctcg gcttcattca tatcggcaag
     1981 ctgttcttgg gctgtaaaag taaaatcttc ggtaggtgaa ggttcttctt tgctttcttc
     2041 gccagccata cgcgcttctt tttgcttttg ctcatattgg ctgacttctt cttctgtata
     2101 gatattcagg cagtattcgc aatgaaattt ttggtctttg ggatcaaaag tcaacggacc
     2161 gccgcaatta ggacatttat gtgtaagaac atccatcagc tgcacacctt tctaaataaa
     2221 ttaattaacg gggattcctt taaacggttt accacagttt ggacagaatt tagggatgcc
     2281 gttggaaaga tccacgatct cattgcattc actgcatcgc atttgtagct taggctgaac
     2341 gttttctaca ggtttggcag ctccgcagtt ggaacagaat tttccggaat tttctgtccc
     2401 gcaaactgga catgtccaag tatccgcatt tccttgtgca gtttgttgat tttgtttttc
     2461 tgcttgttgt ttgatttgtt cttggttatt ttgagctgct tgagaaagat aagaaccatt
     2521 tgcattcatt cccataccta cacccataaa gccggtcatt gctcctgctt cattttttcc
     2581 ggctgcttcc ataccacgtg caatggaacc ttgcacgtat ccttcacgta cgctaggatc
     2641 acctagcatc gccccttcgt tacgcatatt gatcagtttc acagaatcat cggtgtaaga
     2701 aatgctggct acagccacag aaacaatctc catcccacgt aattcacgcc aagaatcatc
     2761 caatgcagta tccatgtatt tgcttaattc caaactctta gatggtacat aagaaatccg
     2821 ttgaccatct gcagacatct gattgattgc tgattgtagc gctgttagga attcagctaa
     2881 atactgctca ttgatatcat tgatttccac ttgtgttttg tttttaggga ttgcgtttgt
     2941 ataaaataaa atcgggtcaa cgacatgaat agaataagtg ccatgtgccc gtaagaataa
     3001 ttctgcgtta tagaaattgt caaagtagtt caacggcgcg cttgtaccga atttgattcc
     3061 ctttatttct tggagattga tataaaaaac ttgttgtttc tgcggtgtga cgccgccaaa
     3121 tttgaatcgg tcaaaagttt cagcaattgc ttctttcagt gttccattga acatggaagg
     3181 agctgcgtca tttttaatgg tgtaataacc ttcctcagcg gtataatcga tgatttttcc
     3241 accatcgacc aaaagcatca tcatgtttgg atagacatgg acgactgttc cgtctgttaa
     3301 aacatcttct gttcctttac gattggagcc acgtttgtca tctttgcgga ctttgacacc
     3361 ttttgtcatg accgttgtat cactcatatt gtctggttcg atgacttcta gccattgatc
     3421 tgccagaccg ccgccaacca tacttgttgc tgctttgata agacccataa aaaattcctc
     3481 ctctgattca gctagtgcca aacaattatt gtttatttat tataccatag gaagatgtga
     3541 aacttctcgg actagagacg gaattgaaat ggaaaattgc gtattgttta aatacctgaa
     3601 aaagccgaga aggatagtag tttttttacc taatatcacg tataataaga gatagtgaat
     3661 tttttaaaaa agggggatcc ttatggcata tcaagcactt tatcgagtct ggcgttcgca
     3721 gcgtttcgaa gacatcgttg gacaaaaagc tgtgacacag acactaaaaa atgctattgt
     3781 ttcccacaaa acctcacatg cctacttgtt tacaggaccc agaggaaccg gaaagacgag
     3841 tgcagcaaag attttcgcaa aagcaatcaa ctgtccgaac agtaaagacg gtgagccatg
     3901 caacgaatgc gaaatgtgtc gatcaattac tgctggtacg caagaagatg tgatcgaaat
     3961 cgatgctgcc agcaataacg gagtcgaaga aatccgattt atccgtgatc gggcaaatta
     4021 tgcgcctaca aaggcagcgt acaaagtgta cattattgat gaggtacata tgctttcaac
     4081 aggagctttc aatgccttgt tgaaaacatt ggaagagcca aaagaaagtg tcatattcat
     4141 cttagcaacg accgaacctc acaaaatacc agctacgatc atttcacgga ctcagcgatt
     4201 tgattttaaa cggatcaata cggcagatat tgtcgaacat cttgaattta tcttgaaaga
     4261 atcgactatc agctatgaac cagctgcatt gaatgtgatt gcccgttctg cagaaggcgg
     4321 gatgcgggat gcattgagta ttcttgatca ggcaatctcg tttagtgaag gaacaattac
     4381 tttagatgat gccatgcaag tgacaggtag cttaactgac gaaatgatgg acagttactt
     4441 gagtgcttgc gtgaaaaaag aagtaccaga agcattggaa acattgaacc aaattctcgc
     4501 agccggcaaa gaatcacgtc gctttctaga ggatatgtta caatactgcc gtgacttgct
     4561 gatgtatcag caagcaccta acctagttga ggaacaatcg agtacgatca gaagtacatt
     4621 tacagaattg gcacaaactg tttcagcgga tcagctgttt tcaatgataa agattttgag
     4681 cgatactcaa aatgaaattc gttttaccaa tagtgccgct atttatttgg aagtcgctac
     4741 agtcaaactt gcacagctac cgtctgcttc tgttccttca gcagatgtag catttgcaga
     4801 tgatcagata gtaaaacagc tacaacaaga gattaaggaa ctaagaaacg aaatcaagga
     4861 actacgcagt aatacaggaa ttgagaatac aaaagtacag ccggtacagc aaacaaatca
     4921 taaaaaacag acaagtacct atcgtgtacc gacggaacga gtatataaag ttctgcggga
     4981 agcgacaaga cagcatctga accaagtaaa agaagtatgg gaagacttgc tgatgagtct
     5041 atctgtcact cagcgagcaa tgcttaaagc aagcgaacca gttgcagctg gacctacagg
     5101 actagtgatt gcgtttgatt atgagattgt ttgtcaaaga gcagcaaatg acgaagaatt
     5161 gcagctttcg gtacacaaca acttaagccg aatgatcaaa gattatgcgc ctgatcctgt
     5221 ttatattaca agagaaagct ggccagaact tcgaagaaat tatttgacgc aggcacaaga
     5281 agagggaaca gaaaactctt ttgcttctga agactcagaa ggagaaatcg agttgcttcc
     5341 tcaagaacaa gaagtaccag aagaaacact tgtagtaaaa gcagaggaac tgtttggctc
     5401 cgaagctgtt acagtggtag atgattaata aaaaggagta gataattatg atgcgtggaa
     5461 tgggcaatat gcaaggcatg atgaaacaag tacaaaaaat gcaaaaagaa atggcagaag
     5521 cacaagagct gctaaataaa acagagttta caggtaaagc caccaatgaa ttggtcaaag
     5581 cggtttttac aggcgatcgt cgcatgaaag atatccaaat caatgaagaa gtcgttgatc
     5641 ctgatgatat cgagatgctt caggatcttg tcgtaatggc agtcaatgat gcgatcgcac
     5701 aaattgatgt ggaaactgag aaaaaattag ggaaatacgc aaaaggtttg ccaggatttt
     5761 agttttaaac tgaaggagta atgaatatgc aatatccaga accaattgcg aaactgatcg
     5821 acagctacat gaaacttcca ggaatcggtc agaaaacagc gacaagactc gccttttata
     5881 cgatcgatat gaaagatgaa gtagtaaatg aatttgctaa atctttgtta agtgtaaaac
     5941 gcgatttgca tttttgtagt atctgcggaa acatcacaga agaagatccc tgcgaaatat
     6001 gtagaaatcc ttcacgtgat cgaagcgtta tcctagtggt cgaagagccg aaagatgtaa
     6061 tggcaatgga aaaaatgcga gaatatcacg gactttatca tgttctccat ggtgtgttat
     6121 caccgatgga agggacaggt ccagaagaca tcaatatttc ttctctgttg cagcggctgc
     6181 atgatgcaga tgtaaaagaa gtgattatcg caacaaatgc aacaacagag ggagaagcaa
     6241 cagctatgta tctctcacgt ctgatcaaac ccgcaggtat caaagtgact cgtctagcac
     6301 atggtctgtc tgtaggctct gatatcgagt atgcagatga agttacttta ttgaaagctg
     6361 tcgaaggaag acgtgagtta taaatttcta attaagtgtt ttttcagata caatataaaa
     6421 gcagcacaag agatgggggc aacaatgtga acggactatt tattacaatc gaggggcctg
     6481 atggtgcagg aaaaaccagc atcttgaatg aactttaccc tcttttgaaa gaagtagcaa
     6541 aaacagaaat cattcaaaca agagaaccag gaggcattcc gattgctgaa gagatccgag
     6601 cagtgatcct tgatccaaaa aatgatcgga tggatgaacg tacggaagca ctgctttatg
     6661 cagcagcaag aagacaacat ctagtcgaaa aggttctgcc tgctctggca aaagggaaaa
     6721 tcgttttatg cgatagattt gtcgacagtt ctcttgctta tcaaggtgca ggccgacgca
     6781 tcggcgttac cgagattgct cggttgaatg agtttgctac agaaggtact acgccggatt
     6841 ttacccttta tcttgacgta gattcagaca ccggattaag aagaatcaag aaaaacagac
     6901 aaaatcaaat cgaccgttta gattcggaag gtttggaatt ccaccagcgt gtacgtcatg
     6961 cttacttaaa gctggcagaa gagaatccgg aaagaatcca caaagttgat gcaagaaaaa
     7021 gctttgaaga agtgctgcaa acaagttatc atacaattat tgaacagtat ccgcaatttt
     7081 ttgaaaacta ggaaggtgaa cgtttatgaa aattatttta gcgattatcc aagacaaaga
     7141 cagcaaccgt ttatcaaatg aattgattga tgcaaatatc cgtgccacaa aactatcatc
     7201 gacaggcggc ttccttaagg ctggaaacag tacgtttatc gtgggtattg aagatgaccg
     7261 tgtagaagaa gcattggaaa tcatcaaaaa aacgtgtgaa tcaagaaagc aatttgtatc
     7321 tacacctgtt acactggata tttcgatgga tgggggcgtc ccttatccag tagaagtaga
     7381 agtaggcggt gccacagtct tcgtcttacc tgttgaagga ttccatcaat tttaggagga
     7441 gctgagctat gaatcaagaa ataaacttgg ctcaaatgca accgatcgtg tatcagcagc
     7501 ttcaacggag ttttgagcat gggcgtatcg ctcatgctta tctttttgaa ggggagaaag
     7561 gaacaggaaa acatgaagta ggtatatggc ttgcacagca cctgttctgt acccaaatga
     7621 aagacaatct gccttgtgga aaatgcaaca attgtcagcg gatcgaaaag aaagaacatc
     7681 cagatgtttt ggttattgaa ccggaaggac aaaccatcaa agtcgatcag atcagacgtt
     7741 tgcagacgga attcagtcgc agcggttacg aatcaagaaa aaaagttttt ttgatcaaag
     7801 aagctgaaaa aatgaatagc agtgcggcaa acagtttgtt gaaattcttg gaagagcccc
     7861 ctggagattt tttagcggtg ttagaaacag atgcagtagg aagaattctc cctactattc
     7921 aatctcgttg tcaagttctg cattttcaag aactttcgaa agaagcactg atccatcgat
     7981 tgcaagaaga gcagattcca ttggaaaaag caaaactttt ggctttctta acaaacagtt
     8041 taggaaaagc agttgaaatc tctcaagatg aatggtttaa tgatgctaag gacttgattt
     8101 atcaatggtt cgtttacttg caaaagcaag atactcaagc ctttatctat gtccaaaaaa
     8161 aattggtaaa atcatttaaa gaaaaaacac aacagtttac aggattatct gtcttgatgt
     8221 tttattatca ggaagcacag cgtcaagcag tagcaaacgg cgaatatggc aaagtgaaac
     8281 agataaacca gataattgaa cggatattga tcgcagagca gaaactacgc agcaatgtca
     8341 gttttcaggc tgttgcggaa cagttcgttt tgcatacgat tttttagctt gccgcaaagg
     8401 atgatgatca aggaggatgg aaatggtaga agtagtaggt gtccgatata gagatgcagg
     8461 tcatatctat tattttgcac caggagactc tgaatatgtt tatagtgaca aggtattagt
     8521 cgaatctcag caatccttac atattgctac tgttgtcctt cctaaaaaag agattgatcc
     8581 caatgacctt cctgaagcta tccgtccgat cttgcataaa gcgtcacaag ctgaattaga
     8641 aaaagacaaa aaaaacaaaa aagatgctta tgatgcattg aaaatcgcaa aagaaaaagt
     8701 gcagttgcgt gagttggaaa tgaaaatggt caaagccgaa tacacatttg atcgaaataa
     8761 attgattttt tctttcacag cggacgggcg aattgacttt agagaattgg taaaagatct
     8821 ggccgctgta tttcgtactc gtattgagct tcgacaaatt ggtgtccgcg atgaagcaaa
     8881 gataataggc ggaattggtc catgtgggcg tccattatgc tgttcttctt ttttaggcga
     8941 tttcgtgcct gtatcaatca aaatggcaaa agatcaaagt ttatctttga atccaacaaa
     9001 aatttccggt ttatgcggac gcttgatgtg ctgtctgcaa tatgagagcg aagaatacga
     9061 gacagcgaaa aaagaactcc ctgactttgg aaaagtaatt gctacacctg atggtaaagg
     9121 gaaagtcgta gggttgaatc tgcttagccg tatcgttagt attcggctga ctggacggga
     9181 aaatcctgtc gaatatgaat gggatgaatt gaaagaattt atccaagtag gtgagatgaa
     9241 tggataagag atcattatac gacggattga atcaattaga atcagagttg agaaatacat
     9301 taacagaact tgttgaaatg aagcatgctt tgcatgagat cgtagaaaaa aatacaactt
     9361 tggagatgga aaatcaacgt ctgcgggaac acctgcggga attgaaccaa gttgcacaag
     9421 ctccagctga aaatgtgaag caggaacttt ctaagtctcg tatgaatctt gaaaaaattt
     9481 atgaagaagg attccatgtc tgctacgatt tatatggatc aagaagggac aatgacgagc
     9541 cttgtgcgtt ttgtctagaa gtgatctatc gcgaaagcgg tcgatgacca ataacaagag
     9601 gagactggag cggagaaata ctgctccagt ctttttatta aggagattgt gacagatgta
     9661 taatcaaaaa agtttcaagg gaaatccggt atatggggat ttatatcttg taccgacgcc
     9721 aattggaaat ttagaagata tgagctacag aagtgttcgt atgctgcaag aatccgatct
     9781 tatcgcaagt gaggatacgc gtaatacaca aaagttattg aatcattttg aaatccatac
     9841 accccaaaaa agtttacatg aacataatta taaagaacgt gtccctcaat tgattgcttt
     9901 gttaaaagaa ggaaaaacga ttgctcaagt cagtgacgca ggaatgcctt ccatcagcga
     9961 tcctggacat gagctagttt tagcctgcat aaaagaaggg atttctgtga tctctatccc
    10021 aggacctaca gctgggatga cggctttgat tgcttcaggg ctagtgcctc aaccattttt
    10081 attttatggc ttccttccac gtaagaaaaa agaacagaaa gaaacattga aaaagctaaa
    10141 agaacagaca gttacgctga ttttttatga gtcaccttat cggatcactg ctacattgac
    10201 gaatttctta gaaatttttg gaaatcgcca agtcgttttg tgtcgggaat tgacaaaggt
    10261 ccatgaagaa taccttagag gaagtacgga agagctactc gcttatatcg aagaacatcc
    10321 agtaaagggt gaatgttgtc tgcttgtaga agggaatttg ttcgaaatgc cagtgacgga
    10381 agttgatgaa gagttgggaa cgttaaagga acaagtagaa gcaaagatcg cttcaggcga
    10441 aaaaccaaat gctgctatca aagcagtagc gctgaaaaat gggctgaaaa aacaagaagt
    10501 ctaccgtcag taccatgaat tagactgaga tagtataagt tgtctataat gaaagactaa
    10561 tgaggaaatc agctactaac ttttcaaatc tcttgaacgc gaacgaatgt tcttgtataa
    10621 tgaataagag gtgaggatga tggagctgca atttccttta cataatgata catcaagaaa
    10681 aatcattcat attgatatgg atgctttttt cgcttcggtt gaagaacgag atcatcctga
    10741 gtttcgcgga catccattag taattgccag acaccccagt gacactggtg ggcgtggagt
    10801 agtgactaca gcaaattatg aagcaagaaa attcggaatt cattcagcaa tgagtgcaca
    10861 aaaagcgtat gagctttgtc cgcaagccat ctttaagcct ggcgaccatc ataaatacag
    10921 cgatatttcc aaacaagtac gggaagtatt ctatcgttat acagatattg tggaaccagt
    10981 tagtatcgat gaagcgtatc ttgatgtgac cgaaaataaa atcaattgca agtcagccat
    11041 caaaatcgcc cgaatgatcc aagcagatat ttgggagagt gtccagctaa catgttctgc
    11101 gggagtaagt tacaataagt ttttggccaa attagcatca gactatcaaa aacctagagg
    11161 aatcacggta gttgcccctc aagacgctgt gccattttta aaagctttgc caattgaaaa
    11221 atttcatggg gtagggaaga aaactgtacc tagaatgcat gagctaggga tttatacagg
    11281 aaaagaccta tatgaatgca cagaaatgat gttgattcga aattttggaa aaatgggcta
    11341 ttctctttat cgtaaagtca gaggaatcca tgattctcca gtaaacgtta caagagagcg
    11401 aaaatctgtg ggaaaagagc atacttatgg gcagccgttg caaacggaag aagcagtgct
    11461 gactcagttg cgccaactag ctgaaaaagt agaagaagca ctgagaagag tccaaaaaca
    11521 tgggaaaaca gtagttttga aggtcaggta cacggactat tctactgtga ccaaaagagt
    11581 gacattgcca gaatatatct ataaaaaaga agcactcttt tatcaagcaa gccttatatg
    11641 ggaggaaatc ttgggagtag aaaaaggaat ccgattgtta gggattactt tgacgaatct
    11701 ggacccgatg acttatgaaa acatcgtcct tccattatgg gaaaatcaag aaatataaaa
    11761 acagctttga gagttgaaac cggaaatccg ttttctctcc caaagctgtt tttatatcaa
    11821 gtcttgcttt ttgatttgtt cataggtttc tgttgcatcc gtacattttt cccaaatcac
    11881 aacttgattg gattctaaag atttttggac gtcaatgatc cgctggttgc tacttccgcg
    11941 aaattgtagt tttaggtcac gtttgcttaa ttcaaagcgt ccgtcaacaa ggatatcgat
    12001 ttgcgaaagg agttctaatt tatcttctga atcttgtagc aattcatcaa aggtatagcc
    12061 tgtccatgac cagatatctt ttgtcgtacc gaactctttt cggatacgtc gtacgacttg
    12121 cagacaaaca tctgtattca aaaaaggttc gccaccaagt aaagttaatc cttgcacgta
    12181 atcgttgcgc aagtcagcga tgatttgttc ttctaattca ggtgtgaatg gtttgccata
    12241 tcggaaattt tgtgcagctt tgttgaagca gccttcgcag gcaaataagc agccactaac
    12301 gtaaagactg ttgcggacac cttcaccatc gacaaaatta aacggtttgt agtcagcaat
    12361 atagttttgg ctgtagtctt ttgctaacca ttcttttggt tttggatttc tcataaaaaa
    12421 acttctttca tttaaggttc aaagatatag ctattgaaga taaaatctac tcgaccacat
    12481 aaataagaac aaactgacct aaatccattg ttcaagaaag cttgttctta ttttgataga
    12541 gggcgttcaa agatacaaag ccatcgaaag agggtggtga aaaaagactt gtcatagcct
    12601 cttattctga ataaacggtg ttcaaaagct tgcatctgca gtaataagcc caactaaacg
    12661 aaaaatatga ggaactattt ttgtttagct attcttatta cttgatgcag gacagctttt
    12721 tcacaacctc atttcatgtg tttcacacgt gaggaaattt ctttatggcg gccatgtacc
    12781 atcggacgag cttgcggatt tcctaaatag ccacaagtac gtttgactac atcacatgtt
    12841 tttggatcat gatttccaca ttctggacat tcgaaccctc gttctgtcgg tttgaaatca
    12901 ccttcgaaac cacattgata gcaatgatcg attggcgtat tcgtaccaag atacccgatt
    12961 ttgtcatatg caaaatccca tacagcttct aaagctttcg gattttgttg gagcataggg
    13021 tattcacagt agtggatgaa tccacctgaa caatattttg gataatcctt ctcaaattcc
    13081 aatttttcaa atggcgttgg gtttttacgc acatcatagt ggaaactgtt tgtataatat
    13141 tctttatctg tgatattttc gatgatgccg aatttttctg tatccaaacg gcagaaacgg
    13201 tcagttagac tttcacttgg agttgaatag acactgaaat gataaccata ttcatccccc
    13261 catgcatctg cattagcttt taattctttg acgatatcaa gtgtgaaagc ttttgcttct
    13321 gggtttgttt cccattcgcc accgaagaaa gctgaagcaa cttcatacaa tccaatgtag
    13381 cctaacgata cagtagctcg acggtttttg aataattcat tgacagaatc ggtacgtgaa
    13441 agacgtttcc cgaaagcacc atacatatat aaaattggcg catttgctgg aatggcttct
    13501 ttacaacgtt ctacacgata gagaagcgca tctttcatga tttctaagcg gtcagacagt
    13561 aactgccaga atttattctg atctcctttt gattcgatcg cgattcttgg caaattcaat
    13621 gtaacaacac ctaaattcat tcgtccgaca tttacttctt gaccattttc gtctttccac
    13681 ccttgcaaga atgaacgaca gcccataggt actttgaaac ttccagttaa ttcaacgatt
    13741 ttatcgtaat tcagaatatc aggatacatt cttttcgttg cacattccaa tgctagttgt
    13801 ttgatgtcgt aattaggatc ttcagggttt aagttcacac cttttttcaa agtgaagatc
    13861 agtttaggga agatagctgt ccgcttttca ctccctaatc cattgattcg aatctgtaaa
    13921 attgcttttt gaatttctct ttcaaaccag ttttctccta gtccaaatcc taatgaagtg
    13981 aaaggagttt gtccatttga agtaaataag gtattgattt catattccaa actttgcata
    14041 gcatcgaaga tatctttttt ggtttttgct tttgcatatt ctttttggcg ttctggcgaa
    14101 tcgatccatt cttccgcgtc ttttagatgt tttttatagt tcaattctgc aaaaggagca
    14161 agcaattcat ctgttcggtc ggcagaacag ccaccatatt gacttgacgc tacattggca
    14221 ataatctgtg agatttgtgc tgtggcggtc tggatagatt ttggcgattc gacttcggca
    14281 ttgccgattt tgaacccatt gttcagcatg cctttgaaat caatcaagca gcaattcgtc
    14341 atcggtgtat aaggatgata atccaaatca tggtaatgga tatcgccttt ttgatgcgca
    14401 tttgctatat gtggtggcaa cattttcagt ccgattgatt ttccaacgat cccggctgtc
    14461 agatctcgct gtgtattgaa aacattactg tctttatttg cgttttcgtt cacgacagtt
    14521 tggtccttgt tgattagctt ttcaatcgaa aagttgatat ctgttgcttt actgcgagca
    14581 aaatctcgtt tggtacgata attgatgtac tcttctgcta attcatattc gtttttttct
    14641 agtaatatat gttccacgat gttttgaatc tcatagattt tgacattttc agcaaaccgg
    14701 cttttgattt cttggacaac caagtctgca atcatttgaa ttttttcata agtaagtggt
    14761 gataatgagc cgtgaatttt cttttctgct ttgatcagtg cttcattgat cttttgttca
    14821 tcgaaatcaa catggcggcc atcgcgtttg atgactttca tctgggatgt ctctggctgc
    14881 tgagtaacga cagttttttc ttcataattt actttcatta tcttcaactc ctgctaaatt
    14941 gttcttgtct atcataaaac aaaatcgggg aaggagcaac taaatatcgg ctatatatag
    15001 tattcaatgt gctgtataat tggatttcta tactatatgt agtgtatatt ttattgtgaa
    15061 aaaaggtgaa aacttgtagc tggtggattt gttttttgca tatttaattt tgctgaataa
    15121 aataattgaa taaaattttt tattcagtcc aagtaaaagt gacaaaaaca taagagcaaa
    15181 ttattcgtag atatgctact atgcaaataa atagaaatga aaaatgaaag aggaggagaa
    15241 gaattggaaa agatgatcga agtgaaacat ttaagtaaaa gctacggcag caaactttct
    15301 cccgtcaaag ccttagataa tatctcattc acagtagaag aaggcgagtt tgttgggatt
    15361 atgggcccaa gtggtgcagg aaagacaacg ctgatgaata tcttgtccac gatcaatttg
    15421 cctacgatgg gcagtgtctc gatccaagga aaagaaatca caaaaatgaa aaacagtgag
    15481 ctcagtgact ttcgtcgaaa aaaactagga ttcatttttc aagagttcaa tttgatcgat
    15541 accctaaatg caaaagacaa tatcctattg cccctagcag ttgaacggat gactaaagag
    15601 gaaatggaaa agagagtaag acatgtagct caattgttga atatagaaga actgctcaaa
    15661 cggtatcccg atgaactttc tgtcggacag agacaacgaa ttacagctgc tagagcactt
    15721 gttgttcagc cacaagtgat ttttgcagat gaaccaactg gctctttgga ctcgaagtcg
    15781 gctacagaat tgttgaacta cttgaagacc atgaatcaaa aagaaaaagc aacgatactg
    15841 ttagtcaccc atgatccata tacagcaagc tactgcgata gaattttatt tattaaagat
    15901 ggtgtgattt tttctgaagt tgtcagaaga gggacaagaa gagagttctt tgagaaagta
    15961 atagacatgc aggcgacgat tggcggagga ggaaaaatga atgctgtata aactagccgt
    16021 taaaagtttt tttcggcagt cacgaggcta tcttgtttat tttttcagtt tgactttatc
    16081 cgtaatgatc tattattcct tcagcgcaat gacctacgat caatctttag tcagaagagc
    16141 aagtcaagat acttcgatag atggtggatt gagttttggt ggatttatca ttactgttgt
    16201 tttgcttttt ttcgtattca gtgccaaccg atttttcttg aatagaaggc aaaaggaaat
    16261 aggcatctat cagctttttg gtatgaataa actgcagata tcaggtatat atgtgttaga
    16321 aatcatgata attggattgt tcgcctgtat ttctgggata ttactcggta ttattttttc
    16381 caaactcttt tcaatgattt tggttcgaat gatggatatg gatcttacta gtccattttt
    16441 tatctccatt ccatcagtag tagatacttt atttgctttt ttcatcatcc ttttagcagt
    16501 atctgtatat tcaatatgga aaatatggcg ctatccaatg attcgaagtt ttggagaaaa
    16561 agaacaagca gatagttcaa caatgcgctt caggacaagg caccgattac tcgcagtcat
    16621 cgggttacta cttttaacga gcggatattt tggagctagt cattttaggg aaatcacttc
    16681 atggatgatc agttatcatt atcttgatac attagttttc ttttttcctt ttttaatttt
    16741 tttcgtttgt gtggtcggta cttatttgtt tttctgttat acatttcggt tatttttgat
    16801 catattgagt aaatggaaga tttattacta tggtatcaat tttttgataa tgggtaacac
    16861 gcaagttcat ttgctcaaaa gttggcgtac aaattctctg attacagtca ttctcggaat
    16921 ttctttaatg acaattggcg gcacggctag tatctctact attttatcct accaggagaa
    16981 agtttcctcc ccgatggact atcagtttga tgttgagaca gagaaaaaaa tcaagccgat
    17041 tctagcagag gaagggcaga agataactaa agaaatcacc ttgcattata aagtcattgg
    17101 gagtatctat agtcaaaatc tcgaagaacc tatgtttcaa actgctaatc tgatcacgga
    17161 aacacaatat caagccttca gaaaagttaa tccagatttg ccgaacattt ctttgaaatc
    17221 acctggtcat actgttttgc ttgatcaaat gcaaactctt tttcggaata tttcattata
    17281 tggatctagt attggactgc cagataatca agaattgaag attcaacaaa tgcttccaag
    17341 tgtattaggt gacgaaacaa tggtatatct aagtcctgta ctgattgtag atgataccgt
    17401 ttttgaaaaa gcaaaaggtg tggattatca agtagtcaat gtagatgtat caggtggaaa
    17461 taatgaaaaa atagatgaac gactgagcaa tgacctgtcg ataaagtggc tcaatgctgt
    17521 ttattattct tatgatatcg taaatggaag tctacaagga gaaattagca caaagaaact
    17581 tccggatgac caaatgaagg aagcacaatt tagccaagaa agctctcgtt tgaatttctc
    17641 ttccagctat tcgaaattgc gtgcagttaa tcggcgaatc ggtatcaata tttttgtcac
    17701 actatttgtc gggatgatcg tgattattgc tactggtagt attttgacag tccggcaatt
    17761 agcagaagca gaggaagaaa aagaaaatta tcacctattg actaagctgg ggattccaca
    17821 aagaaggatc agacggatga tcttcgagca aaacgcactt accttctttc cacccatgat
    17881 attagggatc actcacgcag tatttgcaat caatgtcttc actcagtata taaaaactgc
    17941 cgattactgg ttggcttatc ttgtgagtgg cttattgatc cttatctatc ttttgcttta
    18001 tgtcattacg agtcagttat attgtcggat tatcgaggaa tgatatacgg aataagacaa
    18061 gataataaat agaactaaat tttgaatacc atttatttgg aataagaggt tgtgacacga
    18121 tttttgtcac aacctcttta ttttttatat tttttaaaag tacgagaaat tataaagaaa
    18181 aaagcagtga tttgatgtgc tatttttata aaagctatgt gacaaaactg ttagtaaaaa
    18241 aacggtttct gtaagctaga cacatgtttc tttttgtcaa aattagttac actttgtgtg
    18301 aggttatatg aggagggaat ccaatgaaca aatggcgttc taatccaaaa ttgaattatt
    18361 ggggaaaatt catcattcga acaatttttt atagtgcagt actgctgttc ctgatttatc
    18421 tgtatcacta taaaaatatt caaggaggaa cttttatcta taacgaattt taggaggaag
    18481 agtatggaaa tcaaaacgat tattgaagca atcgatgatt ggggaataaa tgaacctgat
    18541 cgagttgctt atcaggcgga tgagacgcat acttttggtg aattgaaaca agcatcagac
    18601 gcacttgctt attatttaga aaaaaaggta gaaggcgatg gaccaattgt cgtttttggc
    18661 aatttggagt ttgaaatgat tgtctcattt ctaggggttg tcaaatctgg ccatgcctat
    18721 ttgccgattg aagagcatac acccaaggag cgtatcctat cgatctttcg ggtggctaaa
    18781 ccatctatgg tcatcagtat cggggactgg atagaagata ttccttcggt tcctgtcata
    18841 acaaaaaaag aatttcaaga aatcaagaag ataccagtag gattttatcc cagaaatagc
    18901 gtaaaaggta cagaaaattt ttatattatt tttacttctg gtactactgg agaaccaaaa
    18961 ggcgtacaaa tcagtcatga taatctagtc agctttgttc aatggacgat ggaagacttt
    19021 gggatcaagc ctggtatgca tttcttagcc caagcgccat tttcttttga tttatcggta
    19081 ttttctattt atcctgcatt agtttctggc ggaatgctga agccgttaaa taaagcagtt
    19141 gttcaagact ttcgtcaatt attcgcgaca ttgccagcct tgaagttgaa tgtgtgggtt
    19201 tctactccat catttatgga tatttgcttg atggaaccta cattcaatgg agaaaatgtg
    19261 ccggaattgg acatgttttt attttgcgga gaagaattga cgaaaaaaac tgcagaaagt
    19321 ctacttgatc gattcccaaa tgcacggata tataatactt acggtccaac agaagcaact
    19381 gtagctattt caagtattca gattgaccag catgtactag atagttatga ccgactaccg
    19441 attggctacg taaaaaaaga cacgaaagtt tctattgtcc aagaaggtaa acctgtttca
    19501 aaaggagaaa cgggtgaaat cattattgca ggaccaagcg tatcaaaagg ttacctgaac
    19561 aatcccgaaa agacagctgc tgcgttcttt tgtcaaaatg acttagcaag ttaccataca
    19621 ggggatgcag gtagactaga tgaatatggg cttttatttt atgaaggacg tatggatcag
    19681 caggtcaaat tacatggata ccggattgaa ctcggggata tcgaacatta tctgctccaa
    19741 gacaaccgaa tcaaacaagc tgtcgtcgta ccaaaatacc aaggaacaaa agtacagcag
    19801 ctggttgctt ttgtcgtatt agaaaacaaa acgaaacagc cggattttca gttgactaaa
    19861 tctatcaaag aacaattact agaagttgta atggattata tgattcctca gaagttccat
    19921 tatgtcgatc tgctgcctca gacagttaat ggaaaaatcg atcgaaaaaa attaacagct
    19981 gaggtgaatc ctgaatgatg ttacctcata tgatccctta tgcagagccg ttttattttt
    20041 ttcttttagc cattgcgctt gtaccgatcg ttctatcttt attgatatgg gaaaaaagat
    20101 taccagttta tcaatcagca gtgacacttt tctttttatt tgtcagcttt ggaggtaatt
    20161 attggcaaca gggagcggcg ctgattgggt atgttttgtg gcagacgata cttgtatggg
    20221 gctattttgt ttatcggcaa aggaaaaatc agacaggatg gttttttgcc gccgttattt
    20281 tagcgataat tccactgttt ttaacgaaag tgactccttt ttttgaacat ggaaaagcat
    20341 cgttacttgg ttttttagga atctcttatt taacctttaa atctgtacaa atgatcatgg
    20401 aaataagaga tggcatgatc aaaacatata agccatttcg ttatatccag tttctgctgt
    20461 tctttccaac aatctcttct ggaccaatcg atcgctatag gagatttgaa aaagatcagc
    20521 tccatccgcc tgttcgtgat aaatatgttg atcttttggg aaaaggcgtc caaaatattt
    20581 ttgtcggttt tttatataaa ttcattatcg gttattattt tggtcaagta ctgcttcctg
    20641 tagtgggacg cattgcctta cagcatggtg gtatttcatg ggcattagta ggttatatgt
    20701 acgtgtacag tatgtatttg ttttttgatt ttgctggcta tagcttattt gcagtcggta
    20761 caagctacat gatgggatat cagacaccaa tcaatttcaa taaacctttt ttatcatgga
    20821 atatcaaaga attctggaat cgttggcata tgacgctgtc cttttggttc cgtgattatg
    20881 tatatatgcg attgatgttc ttcatgatga agaaaaaatt ttttaaaagc aagatcgtaa
    20941 tgtcaaatat cggttatttt gctttgtttc ttctaatggg tatatggcat ggattgacgt
    21001 ggtactacat cgtatatgga ctgtatcatg cttgtctgat ctgtctgaca gatgcatgga
    21061 tccgatttaa gaagaagcat aaagagcagt tgccttctaa caaatttacg catggcttag
    21121 ccgtattttt gacatttcaa gcagtatgtt ttagcttcct gattttttca ggatttttag
    21181 ataaactttt ttttagctaa cgaatagtga catattaatt tatataacaa aggagaaaag
    21241 gaaaatggaa gaacaaattt tatcaatctt agaagaaata acaggaacag atgaagtaag
    21301 aaaaaatcgt gatattgatc tatttgatga agggttgctt gattctttag catctgttca
    21361 attattagtc gagctagacg gagagctgga tatccaagtc cctgtatctg aattcgagcg
    21421 agaggattgg agcacaccga ataaaatcat tcaacaagca actgcattga agggttgagt
    21481 atgaaaaaga aactttttgc tattttcggc ccggttgttt tagctgttgg cttactgttt
    21541 gtgtttttta cctcctccta tcaagtagat atctctaatc cagaaacgat tagacgtgcc
    21601 tcttcttcca tgtcagtgaa cgtgttgaaa ggagatgcga taaaaaatta tgcattatct
    21661 gaaaaacaat atattccttt ttttggctca agtgaattaa gcagaatcag tccatttcat
    21721 ccgtctgttc tggcagaaaa ataccagcga aactatcgtc catttttgct gggagcaccc
    21781 gggacacaat cgttaagcca atatatgatg atgcgttctg caggagatgc tatgaaaaac
    21841 aagaaagtcg tctttattat ttctcctcag tggttcgtta aaaatggtgt aaaaactgat
    21901 tactttaata cgtattattc tgaacttcaa acatacgact ggttgttttc aatgaagaaa
    21961 gtaacgcctg ctgatcgcta tttagctaga agattattaa ctttctcaaa agtaaaagag
    22021 aatgatacgt tgacggcgat tctgcaaacg atcaaaaaag ggaagctacc tttacctgaa
    22081 tctttgaatc agctgcgcag ccaatggaac atgctgaaga gagaggatga agtattcagc
    22141 aatattgggt tgaaagatcg acagcaaaaa atcgatcatg agtcaaaacg attaccaaaa
    22201 caataccaag aaacagaatt atccatttta gcaaatcaaa ttggagaacg agaaacgacg
    22261 aataatccat tcgggttgaa aaacgatttt tatacgcatc gtatccgagc ccacgagcca
    22321 gagctcaaac agtcacaaaa aaattgggat tatcgttttt cgccagaatt ttctgatttt
    22381 cagctggtct tggatcaatt agcaaaaaac cataacgaag tattgttcat catcccaccg
    22441 gttaacgaga aatggagcga ttataccgga ctatctcaag aaatgttaca aggatttgcc
    22501 aaaaagatca aatttcaatt aaatagccaa ggattcaatc gaatagctga ttttgtcaat
    22561 caagcgggta ccaactattt tatggaagac acgatccatc taggctggaa aggctggttg
    22621 gctgcagatc aacagatcag accgttttta gaagaaaatc atattactgc tagtaagtat
    22681 catcttgacg atgctttttt tagcaaatct tggcaacatc aaataccaga taaactacaa
    22741 ttaaaataaa gaaaacaggc gagtgttgct cgcttgtttt ctttattttt gaatattctg
    22801 aataaaagat aaaaatacca agtttgcctg aacaaaaatc ataagcaatg gtataatcac
    22861 aatatgaata tagaaatggg ggaaaacatt tggatacaaa agaagaacag ctacttattg
    22921 atttaacttc tgcaaaagga gtacctggaa atgaagaaga ggtaagagaa gtattcagag
    22981 aatatgccaa accattcgct gatgatattt tttatgacgg tttaggcagt gtgattgcca
    23041 aacatggtgc tggcggaggc cccaaggtct ttatttctgg acatatggac gaagttggtt
    23101 ttatggtaac aaaaataacc gaaaaaggat tcttggaatt ccaaactctt ggaggttggt
    23161 gggggcaagt catgcttgct cagcaagttg aaatcaaaac acgtgaaggg aaaatcgtcc
    23221 acggtgtgat tggttccaaa ccaccacatg ttttaacacc tcaagtaaga aataaacctt
    23281 atgaaataaa ggatatgttc attgatattg gcgcatcaag ccaagaagaa gccaaagagt
    23341 ggggcatacg tcctggagat atggtcactc catatattga atacaaacga atgaatggca
    23401 gtaaatattt actggcaaaa gcatgggata atcgtatcgg gacagctgtt tctctgcgag
    23461 tattagagaa tctatctaaa gaagcacacc caaatgtttt attcgcagga agcgatgtgc
    23521 aagaagaagt aggcttgcga ggtgctcgaa caagtacaca ccttgtaaat cccgatattg
    23581 cttttgcttt agatactggt acagcaggag acactccagg aatgacacca aaagaagcag
    23641 attcgatttt aggaaaaggc ccacagattt tgatttttga tgcatctatg atcccgcata
    23701 aaaaattatt gaattttgtt attgatgtag cagaggaaat ggatattcca ttccaatata
    23761 cagtgatttc tggcggagga actgatgcag gacaaatgca tttgacaaga gatggtgttc
    23821 cgtctgtagc cattaccgtt cctgttcgtt atctgcattc ccatacatca gtgattcatg
    23881 aagatgatta tttgaataca gtgaaactag tcacagaatt agtgaaacgg cttgatagtg
    23941 aaacagttga aaaaattcgg agttatgaat aatgaataat agaataccta tttctgaata
    24001 tcggaaaaaa gcacgagttt ctttggaaaa tcaatgggga atcaatactt ggcttgtttt
    24061 cttatctata atatttacga gcgtcatcca gaactttttt gacggtttca gtccttttcc
    24121 ggaagaaagc atgcagcagc acttattgag ttttctagtc gataatttac ttttattcgc
    24181 attcaactat gcattgtttt acattgcgct agtagttgta cggggtggca gaccaaaaag
    24241 taacctttta ttttcagttt tccaaagaaa gtattttggt tcgatacttg gcatcaattt
    24301 gatcaattca gtcatcaact ggctactcaa cagtttaatc tttctgccca tgtttttaat
    24361 cagcggagtg agtacttata tgcagctgct tttcagttca aatgcggtac ctacgggtgt
    24421 catgccagaa gattttcttg atatcagctt tgttttatcg atgataggga tgatttttgt
    24481 ttcagcactc atcatgagta ttgttggcgg tttattccaa tttgctgcat ggacaaagtt
    24541 cgactatcct gatttgtcgg ttattcaatg tttgaaatat ggctggtttt tattgaaaga
    24601 tagaattggt acgtatattt tgttgcagct gtcttttatc ggctggtatt tgctaggagc
    24661 aatcgctttg ttcatcggtc ttttgtgggt cattgtttat gtgaatgtgt cagttgcaga
    24721 attttatgaa caagcacgga ttgaaaaagg aggtccagac gcctatttta cttagcatgt
    24781 aagcagttta ttcatcaaaa aaataacttt tgtggtagga taaatctttg agggggaatg
    24841 tacgatgaaa ttaactgttt taggatgctt aggagcgtat ccttataaaa atcaagggac
    24901 aacaggttat ttgcttcaat ctgacgggtt caatttgttg atcgatgcag gtagtgctac
    24961 gctgatcaaa ttacaagaac accttgatcc acttgatcta gatgctgtga tccttagcca
    25021 ttatcatcat gatcatattg ctgaccttgg tgtattacag tattactggc aacttcatcc
    25081 taagagaaat ccggagacat tgctttcaat ttatggacac acaaaagatt ccattcactt
    25141 tgaagaattg acgttggaaa atgtctcaaa aggacatcct tattttgaag ctgaagaatt
    25201 aaaaattggt ccttttctga ttacttttat ggaaacaatc catccagtag tatgttacgc
    25261 catgcgtatc gtagaagaaa caacggggaa agtattcgta tttacaggtg attcaggtta
    25321 tttggaaagt ttcgctgatt ttgcgaaaga tgctgatttg tttctagcag atacctatct
    25381 ttttgaagga aatgaacatc atcatgccca tttcacttca aaagaagctg gagaacttgc
    25441 gaaatcagct aacgtaaaga aacttgtact tacacaccta cctcagtttg gtgatttaga
    25501 acaattgaaa aatgaagcgc aaaaggcagc aggcaaaggg attgatatag aacttgctgc
    25561 agttgacaaa gtgtttaaca tttaggagga agaacatgat ttttgtacca aatgaaaacc
    25621 acgacccaag aatcaattta gcaattgaac aatatttgct gcaagaaatg cctttagatg
    25681 aacctatttt gctcttttat atcaatgaac cttcaatcat catcggcaga aaccaaaata
    25741 cgcttgaaga aatcaatcga gattatgtgg aagaaaatgg cattcatgtt gtccgtcgct
    25801 taagtggagg cggagctgtc taccatgatt ttggaaacct gaactttagt tttatcatgc
    25861 ctgatgatgg ggattcattc cgtgactttg cgaaagtaac acagccaatc atccaagcac
    25921 ttcatgaatt gggtgttgct ggggcggaat tgaaaggccg aaatgatcta gtcattgatg
    25981 gcatgaaatt ttctggcaac gcgatgtatg ctaccaacgg tcggatgttt gctcatggaa
    26041 caattatgtt cgactctgat atcaatgaag tagtcaatgc attaaaagta aaaaaagaca
    26101 aaatcgaatc aaaaggaatc aaatcgatcc gttctcgtgt aacgaatatc aaaccatttc
    26161 ttccagaaga aaagcaaaaa atgacaacag aagaatttcg tcaagaaatc ttgttgaaaa
    26221 ttttcggcgt agagtcagta gaagaagtaa aaacgtacga attgactgat gaagattgga
    26281 aaaaaatcaa tcgaatctct gaggaatatt atcgaaattg ggattggaat tacgggaagt
    26341 ctccagattt caatttctct cgccaaaaac gtttttctat cggttcgatt gaagtccatt
    26401 tgaatgtctc agaaggaatg atcaaagaag caaaaatctt tggcgatttc ttcggactag
    26461 gagatatctc tgacgtggaa aaaaaattgg ttggacagaa atatgataaa gcatctctat
    26521 caaaaatagt cagccaaatt gatgtgaaga aatattttgg tgcgatagaa ccagaagaat
    26581 tgcttacctt actttattga aaataaaaaa tagcggaatc cacatatatg gattccgcta
    26641 ttttttatac aggattcggg taatttcgag agagttgaac agtgatccga tcaatgaaag
    26701 tatctttttc ctgcgttagt gttcctgtgt aacgatccgc tacgtctttc atcatcatta
    26761 agtcaaaacg ttcctgatca tctaattctt ctgtgatgcc tcgactaaat aggtttgtat
    26821 cgtaatgtgc actatgttcg caacaaatga cgaaatgttc ctcttgttcg tgtatcgaca
    26881 aataaatttt tcgatacagt ggattttcga tggcatgagt ttctctaaag gaatgctcta
    26941 aagcgtggat caaaagttga gccagatcgt catcgttgat aggaagttct tcgggaagat
    27001 ctgcttggaa ggttatctga atattccgtt tggcagctaa cttctggtaa cgcgctaaga
    27061 taagatttgt cacctggtgg ttgcaaaaag aacacagcat ttcgaaattt tttgcttctt
    27121 ctaatatctt ttccacatag tttttagctt ttgtttggtc cgtatgcatt aaatcttgca
    27181 gattctccat ctcttggaca aattccaggc gaagtgtatt tacttcttgg atatgctgtc
    27241 gaagctgctc ataatgttgc tccaacaaat catttttgat ttttaagaaa tctacttgtc
    27301 tccgatcttt gtcggtattt aagatgtgat cgattagatt gtagctgata ataacgatta
    27361 gaatgataat atataaaatc gtagaccaat tgatcgttgt ttgatagata cctaatgccg
    27421 tttggatgta aatcaagcaa tggataatgg ctaccaagac gatccaaggt gtacaagcta
    27481 cataaaaacg gttattctta tatgcttcgc caatgcatgc aagtgaagtg actaaagtgc
    27541 ttataacact agcaatatta gcaatcggca ttaattcagg aagttctgca ggagtaaaag
    27601 ttgcataaag cagaacgccg ataaaaatca aaagttggaa cgtacaaaaa attccgtacc
    27661 attttttata gtaaaacatt cggctccaat agtagcagat gatgaaagct gacgttaaga
    27721 taataaataa atgagataaa aaagaatgaa cgaatggttc aaaaagaata ccacttaaaa
    27781 tatcttctga tacagcttca aatcctaatg ccaaagcaaa gcaacttaag agaataagtg
    27841 agtattcaag acgtttttgt ttatacatca agtagatcgt atagaaaaag gtccctatag
    27901 cgataaaaac agcgattatc atcgtcaata cttgcggcac tgattgtgaa taaataaaac
    27961 taatcaatgg tccagttgtt cctagataaa ctttcggtgg cagtccagca tagttctgat
    28021 aaggagatga gactgtaagg gtcaattttt ttccgatata atttgctggt aaatctatca
    28081 cagccaatga gagtccggga ttgctttctt cctgataatc tttccgttgg tataaaatct
    28141 tttcgcctag atatacagta atccattgat ggtttccttt gattagtaaa tttgcttcgt
    28201 ccaacggttt ttcaagtgtc gtttccataa tgaatgtttc gtctttatcc acggtaggaa
    28261 tcgtacgagt gtacttgctg tcaaacttaa aatcaggatc atcttttgta tagaaaaccc
    28321 acgaatcttt tatttctttc atttgattgc tgtccgcagg agaaaaaata aaataaaaga
    28381 aataagcaat tcctgttaaa aataacaatg ataatataat gattatgctc cactttatac
    28441 gtaaataaaa agattctttt ttattatata tgttattaaa aaaagacata aaggtattcc
    28501 tttctaaaac ttgtaatgtt agggtaaacg ttttttaaac taacgtcaat tatttataat
    28561 aaaaaatgag ataaagatga ggaaatagaa ttagaaacat ataattatta aagggaaaca
    28621 tttttacagc tataatttga aaaaaattta tcgttattat atgattaaga aggattgaat
    28681 aaagaaagaa gttgaaaacg atacctgatt acaaaaaaat aacttttata agttctataa
    28741 cgttgattat atttttaatt cttattcttc tcgtcgtctt ggttccccga atttttggtt
    28801 tgactcctta tattattgag gacaaagaaa tgagtccata ttatgcacaa ggaaatctga
    28861 tatatgtacg accgaaaaat acaaatgaga ttttagtcgg agatattatt acatattatg
    28921 aaaattcagg aaaaaaaatc tcgacgcgtc gcgtaatagc cgttgaaaac aaccacgaag
    28981 atttttatac aaaagctgat acaaagactt atattgagcc tggagtagta aaaaagagaa
    29041 atattatcgg tagtcccatc tttcagattc catatattgg actagtcctt tcaaaaacgg
    29101 catttgcatg gattcagggg ttattcttta cgattgcttt ttgcctaaca ggaatcacag
    29161 catggaatac ctttgtagag ttgaaaaaga agcggagacg aacacagaaa tttttgtaag
    29221 aatgttaaaa acataaatgt attccagaga tccaacgact cttctgaaca tgaaacacga
    29281 gaaaacactc atgcagcttg tagatttagt cagttgttcg tataatgtgt gaagaggagg
    29341 agtagtatgc gcaatgaaat gatgcatcgt cctgtagtca aagaagaact attggatttt
    29401 atgcgtaccc agcaaaaaaa attaccgggt gagctaggca aactggaaga agaagctcat
    29461 gtagcagaag taccagttat tccccatgaa acagttgtat ttttgaaatt tttacttgga
    29521 cagcttcagc ctgaacgaat cttggagatc ggagcagcta tcggtttttc atcaagtgtc
    29581 atggccacga caatcggaga aaacgctcat gttacgacaa ttgatcgttt cgatgtcatg
    29641 atcgagaaag ctaaaaaaaa ctatgaacgt ctaggactga cagataaagt gactttgctt
    29701 gaaggtcaag cagcagatat cttgccggag ttgtctggtc cgtatgactt tatttttatg
    29761 gatagtgcaa aatcaaaata tattgaattt ttacctgaat gcttacgttt gttaagaaaa
    29821 ggtggcgttt tgatggtaga tgacatcttc caaggaggaa cgatcttgct cccagatgaa
    29881 gagattcccc gtggcaaacg agctatccat cgtaaattaa atgaatttct tcgtgttgtg
    29941 atggatcatc ccgatttgac atcgacaatc ttgccattag gtgatggtgt gatcttaatg
    30001 acaaaggaat cagaaacaat cgagttgtga tacatgatta tggcagcctg tagataatcg
    30061 attgaatcga gggctcaatt cgaacttgat gtaaaagttt ctatatttgt aagtcaatta
    30121 aaggatacag gaaatctaaa taaacaggag tagcatgaag tcaatgtagc tcctgttttc
    30181 aattgataac cttagaacag ctgaaggaga atgtctcgtt ttaaaagatt gatttagtag
    30241 ttgggccaaa ataattcatt atttgctaat taaataacta tttcgtattg acagaagcaa
    30301 tttaattatc tataattatt gatgttccgc tgattcttgt catagtagct cagctggata
    30361 gagcatccgc cttctaagcg gacggtcggg ggttcgaatc cctcctgtga cgctaaaaaa
    30421 ccgttagaaa ctgaggtttc taacggtttt tctttgtttt ttgtgccaat attgtttgtt
    30481 ttcaaaggca aataaaataa aaaaacatcc ttatcgagta caattataag gagcaaaaaa
    30541 ataaaactca tcagaaaaga ggaagaagat atgacgatac tatctagaat tttagtgact
    30601 ctagttgcaa ttgaattttt ttatatcatg tatatcgaaa cggtgagaac tgattctgac
    30661 acgacaagta gggtttttaa aatgagtaaa gaagagttaa gcagaaaatc tgttcaaaca
    30721 ttgtttaaga accaaggagt gtacaatgga ctattaggag tgggactact ttatggagct
    30781 tatctttctt ccgcatctaa agaaatcaca agtatgctat taatatctat tttttttgtt
    30841 gcattatatg gcagtttgac tagcgatcgt tggattttac tcaaacaagg tgggctagct
    30901 ctgttggcat tgctttctat tttcttataa atatagaaaa aataagaatt tatctaacag
    30961 gaaactttgt gaaaaatatt actttatctc atgaaatatg atattctaat gaatggtatt
    31021 ttagaaaatt tcatgaaaaa ggagttagat aggtgttcaa aaattatgtt tcctcactaa
    31081 aaaaagaatt ttcaggttat gatctttcaa gatttcagaa agatcttttg gctgggatca
    31141 cagtagctgc tgtagcattg cctttagcct tggcttttgg ggtatcaagc ggagctgatg
    31201 cggccgcagg tttgattacg gctatcattg ccggtatcgt tattggcggt ttatcaggcg
    31261 gtttttatca aatatcaggt ccgacaggtg cgatggcagc tatcttgatg tctattgcgg
    31321 caacaaaggg aatgcaaggt gtattgcttg ctactttttt agcgggtcta tttttacttg
    31381 cagcaggact tttccgatta ggaacactga cttcttttat cccagcaccc gtaattacgg
    31441 ggtttacttc aggtattgct atcattattg cattaggtca gatcgataat ttatttggtg
    31501 ttcactcgga aggtacaaac gttattgaaa aactaagtag ttatcaaacg ataggatttc
    31561 cgataaacac agcctctttg atcatgggag ggctagtcat tttagggatg tttatctatc
    31621 caaaaaaatg ggcgaaacga atgcctagtt cgctgctggc tatcattttg gcaacagcag
    31681 ttatgctatt gttccattta ccagtagcaa ctgtgggtaa gattccacaa acattgatca
    31741 gtagtaatcg tttgaatatg ggagatttta gcttttctgc cttgcaacaa gtggcagttc
    31801 cagctattag cattgcttta ttaggaatga ttgaaagctt gctatgtggt gcttcggctg
    31861 gccgaatggc aaataagccc ttagatagca atcaagaatt aatggcgcaa ggaattggaa
    31921 atctgttgct tcctttcttt ggcgggattc ctgctactgc ggccatcgcc cgtacaagcg
    31981 tggccatcaa atctggtgca caaactagat tagctggtat gatacatgca gttgtcttac
    32041 tgctctccat gcttgttttt gcgccagtga tgtcacagat tccaatgcca gcacttgcag
    32101 gtgttttgat tgtgacggct tggcgtatga atgaatggga aacgatcaag gagctattta
    32161 ctaataaata ttggtcagca gttctattgt ttatccttac gatgatttgt acagttatct
    32221 ttgatttaag tattgctatc gtgatcgggg tgatcggcgg ctgtgtattt tttgttgtca
    32281 aaagcgctgc tatcacgatt tcattagaaa cgatcgattg gcaaagaatg gggctgccag
    32341 aaacggaaaa gctgaataac tggacagtag tgtatattag tggtccactg ttttttatgt
    32401 cttcagaaaa actcaaaaat aaattggaag aactaaatca tcacgatggt gtcattttct
    32461 ccatgcgagg cgtaccaagt attgatttga cggcacgtgc agtttttgat gaatttcaag
    32521 aaaaagcaga gcaaagagga caaacgatta tctatacttc cttgcagcca gaagtagaaa
    32581 aacaactttc tcacttgtgg gaacatacat tcacgcatca acatcaaaca gttgctcatg
    32641 cgttgacagc tttacatgcg aaatatactg caattagcca ttaaaacaat gaaaaagaac
    32701 agacaagact gtaaactgaa gattttatgg tcttgtctgt tctttttaat attgctgtaa
    32761 aagtgaagct agtacttgcc agtcttgatg gataacaggt acaagggagc gattgtagaa
    32821 aatagctgct ggatgaaaaa gtggaagaat tcgatagttt ttttctgacc attcgtaggc
    32881 atccttttgt tctgttaatt gtaagacagg ccctgtatag agttgaccat gatttttggt
    32941 aaccgtatat tctttgccaa gtaaacgttg aagaccaatg tttccaagtg tcgcaattaa
    33001 ttttggctgg accttcatca gttcgtaatc caatattggt gcatgcgcaa atacttcgga
    33061 acgagtaggt gtccgatttg gataaacagt ttctgactgt tgtgtccgtt tattgatccg
    33121 atgagtcacg cgatacgggc ggctccttac agcactggtg atatacacct cttctcgtgt
    33181 taagccagtt gaagaaagtg cctgcatcaa ttcttttcca gcttgaccgc taaaaggaac
    33241 atgcgtatca atctctgttt tccctggggc ttctcccacg agcatcagtt taggatgaag
    33301 aggtccttgc ccagcgataa atccttctac aggacggccg ttcattctta aagaagcttc
    33361 ttgtatcagt tcttttgggt attccattta tgctcctcct ttttctttta ttataagaat
    33421 aaaagaaaca caatgcgact attttatttg gtagaactgt gaggatagaa aagcagaagt
    33481 attgcttgaa tttttgtttt actttttgat tttaaattta gttgactaac ggttgctgtt
    33541 ctggtaagct agtactgttg taattgtgga ctccacagct acaaccgctc agaacaagta
    33601 ataagcacag catatgctgt cacttcggat ggcgagtcta agtatatcag aaggaggtgc
    33661 tttatagcat gtacgcaatt attaaaactg gtggtaaaca agtaaaagtt gaagtgggac
    33721 aagcaatcta tgttgaaaaa cttgacgttg aagcaggaga aaaagttgtt tttgacgaag
    33781 ttatcttagt aggcggagaa tctacaaaag taggcgctcc aactgttgca ggtgcaactg
    33841 ttgaaggtac tgttgaaaaa cacggcaaac aaaagaaagt cgttactttc aaatacaaac
    33901 ctaaaaaaca ttcacaccgt aaacaaggtc atcgtcaacc atatacaaaa gtggttatcg
    33961 acgcgatcaa cgcttaatag cgtttgagaa agaaggccta tatatgatca aaggaacatt
    34021 taagcgaaat gacgcaggtc agatcgtttc attcactttg accgggcatg ctgatgctgg
    34081 tccttatgga agtgatatcg tttgtgcggg tgtttctgcg ttagcgatca gcactgtcaa
    34141 cgggattgcc tcattagccg gttttgagcc gattgttgaa atgaatgaag aagaaggcgg
    34201 ttatctttat accgaagtca cttcaggaat gacacaagaa caaaacaata ttgctgagat
    34261 tctattagaa aatcttttat taggattaca atcgatagaa gctgaaaact cagaatatat
    34321 ccaaattaaa acaattaatg ataaatagga ggtgcagacc atgttgttat ctatgaatct
    34381 acaattattt gcccacaaaa aaggtggcgg ttctacatct aacggccgtg actcagaatc
    34441 aaaacgtcta ggtgctaaac gtgctgatgg acaaacagtt acaggtggat caattcttta
    34501 ccgtcaacgc ggtacaaaaa tctatccagg tgtaaacgtg ggtatcggtg gagacgacac
    34561 tttatttgca aaagttgatg gcgtagtacg tttcgaacgt aaaggccgta acaaaaaaca
    34621 agtgtctgtt tatccagtag ctcaagaagc ttaattgaat cttatgatta aagaggttgt
    34681 gacaagactt ttgtcacagc cttttttgtt tataaaaata aaagttgtag tggaggctac
    34741 gaagtttact ttgtcttttt acacttaata tttttttgcc aatttggaat caggatgttt
    34801 tgataactat cgttttcaat tgatggaagc tatattgttt gctatcagtg aaataattat
    34861 atggtaaaca gtgttctttt tcttgtttcc attataaaaa tcgtatcggg atttttcgtt
    34921 cttttaaatt gaacatttct atctatccgc gtgttatcaa caaaacaaaa tcaggtaaat
    34981 agttgacgaa acagaaataa atacatagta ttatgttttg aagcctaagt acaatagtct
    35041 atagaaaagg agcgataaca ttggcaaaac gcattgaccg aatttatatg tatgtcaaag
    35101 aaaaaaccgc tcatttaaca aaggcagaat atgatcaagg agtaacaaca caggaaattg
    35161 ctgaagtgtt agggattcag cgaacaaata gcagtaaaga tctgaatcag ctagtgcgcg
    35221 aaggaaaact gataaagaca gacggacgac cagtacgtta catatatcaa acactcgtta
    35281 cgcacgtaaa gccagttaca aaacatgtag tcagttacaa agaaacgccg gtctctgaag
    35341 aaaaaaatac actccaaata gatactaagg atatctttgc aaaaatcatt ggagctaacg
    35401 gcagcatgaa aaattcagtg gaacaggcaa aagctgcgat tttgtatccg ccaagaggat
    35461 taaattgttt gattacagga cctaccggat caggaaagac ttattttgca cacgcgatgt
    35521 atcattttgc aaaagcgcat catgtaatag atgaggaaac ggaactgatt gttttcaatt
    35581 gtgctgatta tgccaataac cctgagcttt tgatgagtca tttatttggg tacgcaaaag
    35641 gtgcttttac aggcgcagat gaagaaaaaa tggggattat cgatcaagcg gatggcggta
    35701 tgctgttttt ggatgagatt caccggttgc cgcctgaagg tcaagaaatg atcttttact
    35761 ttatggatca tggaacttac agtcgtcttg gggaaacaac gaaatcccat gaagcaaatg
    35821 tacggatcgt aggtgcgacc acagaagacc caggttcttc tctattagaa acttttgttc
    35881 ggcgtatccc aatcaatatc aaacttccgg cttttgaaaa acgtcctgca aatgagaaag
    35941 tcgatttagt aaaaatcatg attgcccacg aagcaaaccg cacgcagcga aagatttctt
    36001 tgacagaaga tgtagcaaaa gcattgatcg gcagtgtgac ctatggaaat ataggacaat
    36061 tgaagtcaaa tatccagtta gtatgtgcac gaggatttct aaatcatatg caatcacccg
    36121 aaatctcgat cacaatcgat gatttgacag aagggattcg tagcggattg attcaattag
    36181 caagcaaccg cagtgcaatg gcagagcttt ctaaaatatt ggagccaaag ataactgtag
    36241 caccaaatga tacgatcatg aaaatccaat cggactccta tgagttacct tataatctat
    36301 atgatatcat cggagataaa gcagctttac tgaagtcgga tggtttggat caagaagcaa
    36361 tcaatcattt catttctaca gatatcaaca tccatctgaa gtctttctat aaagaccatg
    36421 gtttttcttt taatgcagac agcaaattag cagaatttgt agatccgaaa atcatagaag
    36481 tgaccaatca gatttatgta atggtcaaaa gatctttacc atatgagttt cagcaaaact
    36541 ttatttatgc tatgagcttg catatcagtt cttttttgaa aaaaatcaac ttaggagaag
    36601 aacggcatac gaatgataac atccgtgaga tggcttttga ttttccagat gaatatgctg
    36661 ttgcgaaaga agtgcgtagt tatatcgaaa cgtattttca agtggagatt ccagaaagtg
    36721 aagactatta cttgaccgtt ctactcgtct cgttacgggc aaatcaagca agcggcagaa
    36781 tcggtgtagt cgttgcagct cacggaaaca gcacagcaag cagtatggtc caagttgtcc
    36841 aacagttgtt agatgcagag aacgtacgag cagtagacat gcctttggat atggatccga
    36901 aaacagcatt ggaccgaatt gaacgcaatg tacaagaagt agatgaagga agcggggtca
    36961 tactcttagt agatatgggg tcccttgcgt cgtttaattc gcaaattcaa agagacactg
    37021 gaattccagt acgtactgtt gatatggtaa caacttctct agttttggaa actgtacgaa
    37081 aggtctcagt attagggaca gatctagata tgttgcatga atcattgaaa aattttcgag
    37141 gttacgcaga aatatcttct gtagaatctg ttcaggaagc aaaacctatt cataagaaaa
    37201 aagctgtttt ggctatctgc gcatctggcg aaggtacagc acaaagaatc aaagaattga
    37261 tcgaacgagc ggtaaacaag cgtcaagaaa cagaattaga agttctagct ttgtcgattg
    37321 tggaagtgaa agaagaactg ccagttatcc aaaaagaata tcagatcata gctactactg
    37381 ggattactga tttgaatatc caagccccat ttattccttt agaacgtttt attgatcaaa
    37441 atatcgaagt cattttggat cagttgctga tggaatcaga attggaggaa actgaattca
    37501 tctccctaga tgaagagtca gctaaaaata cgtgtgttga gtttatctct gataacttca
    37561 tctttattaa tggtagtaag ttaatcgatc caatgtggca attttctaca caaatctctc
    37621 agacgacggg aatcggcgat gaagaatatg gattcaaaat caacctagtg atgcacacag
    37681 caggaatgat cgaacgtatt attcggaatg aaccattgac tgtggaagaa aacgaattga
    37741 caaatacaac gaatgatcct ctttatagcc aattggcagc ctcagttgtt ctgttggaag
    37801 atcagataaa agtaaaagtg ccgatagaag aaatgtatta tctgctacgt ctagtccata
    37861 atcagttaga taaaaaagaa tacactgtgc catgaataca caaaaacgat acacttttat
    37921 agtgtatcgt ttttgtgtat tggttaagaa tcctttaaat tcaattagtg tatcgtaaaa
    37981 aagttggcac gctttttgct tatagtagtg tgtgctagga ggtggttgtt cacacagaaa
    38041 tgataggggt ttctgtgaag tagtgaggaa gaaggagttt tgcagatgga tattcgattg
    38101 gcacgtatag atgatcgttt aatccacggg caagtcgcaa cagtttggtc gaagcaaaca
    38161 ggtgttgaac gtatcattgt gatcagcgat gtagtcgctc aagataaatt gagaaagttt
    38221 ctactaaaag aagctgcacc gccggggatc acagcaaatg taataaccgt gcagaaaatg
    38281 attcaaatct atcatagtgg attattatca cgagcaaaag tgatgctttt attcaccaat
    38341 ccccaagatg ttgaagcggt tgtccgctgc ggtgtacagt tgaagtcgtt gaatatcggc
    38401 ggtatgagtt ttactgaagg caaatcaatg attaccaatt ttgtttcagt aaatcaacaa
    38461 gatattgatt cctttcgtta cttgaacagt caagaagtgg agttagagat tcgaaaagta
    38521 cctgctgaca ggaaaatcct tttgatggaa ctactagcga aaatcaaaaa ggtgtagcaa
    38581 gaatggttgt tggaaaaggt caaataattc tgttgtaatg gttagcaatt attaatattt
    38641 ttgaatttgt tacaacaaaa gtaaatagga ggtataaata tggtagggat tatcctagca
    38701 agtcatggac aatttgctga aggaatctta caatccggtt caatgatctt tggcgaacaa
    38761 gaaaacgtca aagccgtgat tttgaaacca agtgaaggtc cagatgactt gagagccaaa
    38821 ttggaagaag cggttgcttc ttttgataat caagacgaag tgttattctt agttgattta
    38881 tggggcggaa caccgttcaa tcaatcaaac acattattcg aagagcacaa agataaatgg
    38941 gcaatcgtca gcggtttgaa tttaccaatg ttgattgaag cctacgcatc acgcttttca
    39001 atggaatctg ctcatgaaat tgcagcacac atcattgaaa cagcaaaaga cggtgtaaaa
    39061 gtaaaaccgg aagaattaga accggcagaa gcaccgaaag cagcagttga agacgcacaa
    39121 cctaaaggag cacttccaga aggaactgtg gtcggagacg gaaaaatcaa atatgtatta
    39181 gcacgtgttg actcacgtct gttacatgga caagttgcga cagcttggac aaaagcagtg
    39241 caaccaaacc gtattatcgt tgtttcagat gcagtatcaa aggatgatct gcgtaagaga
    39301 ctaatcgaac aagctgctcc tccaggtgta aaagcaaatg ttattcctat tagcaaaatg
    39361 atcgaagtag caaaagaccc tcgttttgga aataccaaag cattgctttt atttgaaaat
    39421 cctgaagacg tattgacagc tgttgaaggt ggcgtagaca tcaaagaatt aaatgttggg
    39481 tcaatggcgc attcggtcgg taaagtcgtt gtaagtaaag tattgtctat gggacaagaa
    39541 gacgttgaag cgtttgagaa gcttgaacaa aaaggtgtga aattcgatgt acgtaaagta
    39601 cctaatgatt cacgcgataa catggatgat attttgaaaa aagcaaaagc tgaattagca
    39661 aaagcgtaat aaataatatt aaaatttata ggaggcttat catgtctatt atttcaataa
    39721 ttttagtcgt attagttgcc tttctagctg gtatggaagg tattcttgat gaatttcaat
    39781 ttcatcaacc actagtggca tgtacattga tcggtttagt taccggtaac ctcgaagctg
    39841 gtatcgtact tggcggtacg cttcagatga tcgctttagg ttgggcaaat atcggtgccg
    39901 ctgtagcacc agatgctgca ttagcatcag tagcatcagc aattatttta gttttaggtg
    39961 gacaaggtgt taaaggtgtt ccttctgcga tcgcgattgc cgttcctttg gctgttgcag
    40021 ggttgttctt aacaatgatc gtacgtacaa tcgctgttcc aatcgttcat ttgatggatg
    40081 cggctgctga agaaggaaac atccgtaagg ttgaaatgtg gcacatcatt gctgtatgtt
    40141 tacaaggggt tcgtatcgcg gtcccagcag cagcgttgct atttatccca gcacaaactg
    40201 tgcaatcatt ccttgaatca atgccagcat ggttaacaga tggtatggct atcggtgggg
    40261 gaatggtcgt agctgtaggt tacgctttgg taatcaacat gatggctaca aaagaagtat
    40321 ggccattctt cgttattggt ttcgttgtag ctgcaatttc tcaattaaca ttgattgctc
    40381 taggtgcttt aggtgttgca ttagctctta tttacttgaa cctttctaaa atgggtggtt
    40441 catcaaataa tggaggcgga agcaactctg gtgacccttt aggcgacatt ttgaatgact
    40501 attaatcttg aaggaggaaa ataaaatggc agaagaaaaa atcaaattat caaaaaaaga
    40561 tcgtttagct gttgcgtggc gctctacctt cattcaaggt tcatggaact acgaacgtat
    40621 gcaaaacggt ggctgggcat tttctatgat tccagcaatc aaaaaattat acaaaacaaa
    40681 agaagaacgt tctgcagcat taaaacgtca cttggaattc tttaatacac acccatacat
    40741 tgcttctcca attttagggg ttacattggc tctggaagaa gaacgtgcga atggcgcgcc
    40801 tgttgatgat gtagcgattc aaggggttaa agtaggtatg atgggtcctt tagctggtgt
    40861 tggtgaccca gtgttctggt ttacagtacg tccaatgcta ggtgcactag gtgcttcatt
    40921 agctatggga ggaaatatcc ttggaccaat catcttcttc ttaggttgga acattattcg
    40981 ttgggcattc atgtggtata cacaagaatt tggctacaaa gctggttcta aaattactga
    41041 tgacctttct ggcggattgt tacaagacgt aacaaaaggt gcttcaattt tagggatgtt
    41101 cgtattagct gcccttgtac aaagatgggt atcaatcaaa ttcttaccag ttgtatcaac
    41161 tgtaaaatta gataaaggtg cttacatcga atgggataaa ttgccttcag gtggtgaagg
    41221 aatcaagagt gcatttgaac aagttaataa tggcttagcc ctttctcctg aaaaggtaac
    41281 aacattacaa gacaacttag accaattgat tcctggttta gctgcattag gattaacatt
    41341 gttatgtatg tggttgctta agaaaaaggt ttctccaatc gttatcatcc ttggtttatt
    41401 cgtagtcggt gttgttggac acgtcatcgg acttttataa tccataaaaa tatagctcgg
    41461 gcgttgtccc gagctttttt tatatagtat actgttcata aacaaatatc tggagggaga
    41521 aaatggttca atcaatcaat acaaaagtag acttggtaat tgatgctact tcgtttacgg
    41581 gtctgacaga ttacgggcaa atcatgatag gtgataatgg ttttgagttt taccatgcgc
    41641 gagatagacg caaatatatc caaattccat gggaagaagt ggattatgta ctagcatcag
    41701 tcatgctaaa gggaaaatgg attcctcgat atgccattca aacgaaaaaa aatggtacat
    41761 acacattttc ttccaaagaa cctaaaaaag tgcttcgtgc gatcagagaa tatgtagatg
    41821 aagatagaat ggtacaatca ttgagtttct ttgatgtagt gaaacgtggt attcgtagta
    41881 tgtttaaacg caagtaaaaa ataaagaaaa cttctaagag gaggttttct ttattttttt
    41941 actttgcaga agaaaatatg aaatagcaaa accaattatt tgtccccttt aaatcccata
    42001 tgctaaaatt gattagcaaa gaaggaggtt atattatgag ttggttatgg acattgattg
    42061 ttggagccgt gatcggagca attgcaggtg cgatcaccag taaaggaaat tcgatgggct
    42121 gtgtaacgaa tatcattgct ggactagtcg gttcaagtat tggacaagcg attttcggcg
    42181 aatggggacc aaaagctgca ggtatggcac ttattccatc tattttaggc gcagtgattt
    42241 tggtagcagt tgtctcattt ttctttggaa aacgatcata agtttttaca aaatagtaaa
    42301 ctaaaaggag ttgcagcgag actgtgactc ctttttggct ttatttattt aaaataaaaa
    42361 gaagttttcc ggaaattttt atttaactgt cgtgtacgaa gaaaagtttg ctataatgga
    42421 aaaaagcgtg caaagaaaga gggagtttca ttatgatgct acgtgtagaa aaattgcgca
    42481 aaaaaatgca agaagaaaat ttggattcat ttttggttac cagtccgtac aatcttcgtt
    42541 acttaacgaa ctttacaggt acgacaggat tagctgttat tactttggaa aaagcatttt
    42601 ttattactga ttttcgttat accgagcagg cggcagcgca ggctcaaggg tttgaaatta
    42661 ttaaaaatgt cggacctatt tttgatgagg tagcaaactt agtacgcaaa gaagaactgc
    42721 actcattagc ttttgaggaa acaacagtct catttttaga atattcggtt ttagaagaaa
    42781 tcatcgatgc ggaattagta cctgtttcac agatgatcga agaactcaga gaagtgaaag
    42841 atgaagaaga aatcgctatt atcgaaaaag catgtcatat tgcggatatg gcatatgatc
    42901 acatcctgaa aatgatccaa ccgggcatga ctgagattga agtcgcaaat caactagact
    42961 tttatatgcg ttctcttggc gcttcaggtg tttcttttga tacgatcgtt gctagcggat
    43021 tgcgttcggc tatgccgcat ggtgtagcaa gcaaaaaaat catcgaacaa ggtgacttga
    43081 tcacattgga ctttggttgc tattacgaag ggtacgtttc cgatatgaca cgtacattcg
    43141 ctattggtga tcctggggaa aaattaaaag aaatttatca aatagtctta gatgctcaat
    43201 tagcagttat tgatgctgca aaaccaggaa tgactggtgt ccaattagat gcagttgcta
    43261 gagattatat ttcaaaacat ggttacgggg aagcatttgg acattctaca ggacatggga
    43321 tcggtttaga gatccacgaa ggaccaaacg tttcttctcg tgcagaaaag caatttgtac
    43381 caggaaatgt gatcactgat gaaccaggga tctacttacc aggaatcggt ggagtacgta
    43441 ttgaggatga cttgttgatc acagaagatg gaaatcgtgt gttgactcgt tcaccaaaag
    43501 aattgatcat cttatagttt catcaataga agaagtaagc gtaaaaatca gtgttcacag
    43561 actttgttat agaaaatcac tcgatttaat gatacactat agaagaactt ttaagctgta
    43621 agagataata aagttgggtg ttttctccaa ttcatagcag tttgcagctg atcggcggca
    43681 gttttcttac cacgtgatca ctatgccgca tcatagagaa ttcggatgta tctcgtgctt
    43741 aggcaaaggg aggaataaat aaatggcaga tgaaaaaaat ttgattttaa acgcaaacca
    43801 agatttagga gagatcgtga tcgctcctga agtcatcgaa gtaattatcg ggattgcagc
    43861 ttctaaagta gaaggcgtat acggaatgcg cggaactttt gccagcaatg tcactgaatt
    43921 attaggacgt gcagctcacg gaaaaggtgt ttacttagca aacgaggaag atggcttgaa
    43981 agtagatctt tactgttatc tagaatatgg tgtttcagtt cctaaagtag caatggaaat
    44041 gcaggaacgg gtcagccagc aagtcctttt catgacggat atcgatttat ctgaagtcaa
    44101 tatccatgta gtagctgttg ttcctgaaaa attagaacaa ccaacaatcg aagaactttt
    44161 tgaagacgaa gaggaagaga atgaataaag aattatcaag acacgagatt cgggaaatgg
    44221 cattgcaggc tttatttcca ttagacttca atgcagacct aacaaaagaa gatgcgattt
    44281 tcaacgcaat cgaattagac caccgcgaca tgatcaacga agatgaatca gagtttgttc
    44341 ctgtttatct tgatacgcta gttggcggtg tctgtgcaaa aaaagatgaa ttggataaag
    44401 tgattgaaaa acatttaaaa aacaattggc acatcaatcg gatctcaaaa atggatttgg
    44461 tgatcttgcg tatcgcgatt tttgaaatga cttatgtgac agatgtacca gcggctgttg
    44521 ctttaaatga agcaatcgag ttggcaaaaa catttagtga tgaccgttca cgtaaattcg
    44581 tcaacggtgt tttatccaat gtattgaaag aattagaagc tggggcgtaa gtcctagctt
    44641 ttttctatga atcatcataa acctgttata aatcgggaaa attctatggt agaatgaaaa
    44701 cacaaaaaat atctgggagt ggttagatgg cagaattgat taacggaaaa gaattagctg
    44761 aaaaaatgca ggcagagatt gctgaaaaaa tccaaaaatt gaaagataat ggtatccatc
    44821 ccggattagt cgttttattg gtaggggaaa atccagccag ccaaatctat gtaagaaata
    44881 aagaacgtgt agcaaaagaa atcggaatcc attcattggt agagcgttat cctgagacga
    44941 tttctgaaga cgaactactt gcagaaattg aaaaatacaa tcaagatcct agttttcatg
    45001 gtatattggt ccaattacca ttgccaaagc atatagatga agaaaagatc ctgttagcta
    45061 tccgccctga aaaagacgta gacggttttc atccgatgaa tttaggcaga ctccttgcgg
    45121 gtaaaccgga caagattcct tgtactccat acggcatcat gaaaatgttt gaagcttatt
    45181 ctatcgatcc ggcgggtaaa cgagcagtag ttatcggacg aagcaatatc gtcggtaaac
    45241 cgatggctca gctactcttg atggcagatg caactgttac tgttgctcat tctaaaaccg
    45301 aaaatctggc tgaactggca caagaagcag atattttagt cgtagcaata ggacgtggac
    45361 attttgtcac aaaagaattc gtaaaaccag gtgctgtagt gatagatgta gggatgaatc
    45421 gtgatgctaa cggtaagctg atcggtgatg tgaaatttga tgaagtggag cctattgcag
    45481 gctggatcac tcctgtgcca aagggtgtag ggccaatgac tatcacgatg ctgatgtatc
    45541 aaaccgtaaa atctgccgaa gcttttggag caggtgaata gatggcacaa gaatatttaa
    45601 ctgtcactac attaacgaag tatttgaaaa gaaaatttga tgccgatcct tatttagaac
    45661 gcgtatattt aacaggcgaa atatcgaatt tccgtttacg tgccaatgcc catcaatatt
    45721 tcagcttaaa agatgaccat gcaaaaatat ctgctattat gttcaaagga gcattccaaa
    45781 aattacgatt ccagccaaaa gaaggaatga aagtccttgt cgttgggcgt atttcattat
    45841 atgaagcggg aggttcttat caaatctatg tagagcacat ggagcctgat ggaataggtg
    45901 ctttatatca agaattggaa gaacgaaaag aaaagctgat gaaggaaggc ctttttcaag
    45961 ggcctaaaaa aatgctgcca cgtttcccaa agcgaatcgc ggtccttacc agtccaagcg
    46021 gagcggttat ccgcgatatc atcacgacgg tgaaaagaag atatccaatt gtccaattgg
    46081 ttttgttccc aacagttgta caaggaaacc aagcagcgga caatgttgtc aggaatatcc
    46141 agagagtaga agagatgggc aatttcgata caatgatcat cggtcgtggg ggtggttcga
    46201 ttgaggattt atggccgttt aatgaagagc gggttgctcg ggcgatttat caagctaata
    46261 cgccggtgat ttcatctgta ggacatgaaa cagataccac gatcgctgat ttagtagcag
    46321 atgttcgggc tgccactcca acagcagctg cagaattagc tgttcctgtt ttgtcagaag
    46381 agttgatgcg cattcaagaa cggcaagcta gattgcagca ggcgtttgta cgccagatcc
    46441 agcgcaaaca agaacggttc gagcgtgctc gacgttccta tgtatttcgg cagcctgaaa
    46501 gattgtatga aggtcagacc atcaaattag accagttgaa acagcgatta tatcaagcta
    46561 cacagcaaat ttatcatgcg aaagaaaaac aaacgatcgc attaagccac caattggaac
    46621 aagtggctcc gctttatcga gttaaagcag ctcgccaaga aactgcttat ctagaaaaac
    46681 gcttagaaga gaaaatccaa caatatatga aacaacagca gcagcgtttt gctcaagctg
    46741 ttcaatctct tgatttgttg agtccattga aaatcatggg aagaggctac agctatacga
    46801 cgttaaatga tcaagtagta aaaagtgtca ccgagattca tcaaggggat ttgctgacga
    46861 tccattatca agatggaatc gttaaaggac aagtacaaga gatagacaaa ggagaataaa
    46921 aatatggaaa atccaacttt tgaagagtca ctgcaagaat tagaaaaaat tgtcatgcag
    46981 ttagaacaag gagacgttcc attagaatca gctctagatt cgtttaaacg aggaatggaa
    47041 ttaagcaaac attgccaaga tacattagct aaagcagaag agaccttgac gaaaatgatg
    47101 acagaatcaa atgaagaagt tgattttgac ggaaacgagg aaagctgaga tgagattaac
    47161 tgacttttca gctattcatc tacctgctgt agaaaaagaa atcttgtctt ttttagatga
    47221 gcatacaacg gatagaggac tctatgatgc gatgagttat tcagtgaaag caggcgggaa
    47281 aagaatacgt cccttattac ttttaacggc cgttgcttcc tttgatgagc ctattgatgt
    47341 tcccgtctat caagtagcag ctgctttaga aatggtgcat acgtattcct tgatccacga
    47401 tgatttgcct gcgatggaca atgatgatct tcgtagagga aaacctacca atcataaagt
    47461 attcggcgaa gcattagcta tattggccgg agatggtttg cttacaggag cattccagct
    47521 aatcagtatg gcacacttag gtaattctcc taagcttctg ctgcttcagc aattagctgt
    47581 ttgtgcggga tcacaaggta tggttgctgg gcaagctgca gatatcgagg gcgaatcgaa
    47641 aaagctttct ttagaagaat tagcatttat tcatgaaaga aagacagggc atttgattcg
    47701 ttatgccttg ctggctggtg gcatattagc gaagcaacct gaagaaatct tattgctact
    47761 tcaacgatta gccgaacatt taggtttagc ttttcagata agagatgatc tactcgatgt
    47821 gattggtaca acgaaaacat tagggaaaac agctggtaaa gatgaacgta tggaaaaaaa
    47881 tacttacccg cgtttattag gcctagaaaa gacacgtgag gcgttggaga ttgagctgtt
    47941 atccgccaac aagatcatag acaaattaga agaggacgtt ttttcttttg atggcgggct
    48001 gatgcgccag atgatcaagc aatttgatgt ggagaaatga gggaaagaat ggaaaaagaa
    48061 cgcgtagatg tattagcggt caaacaagga ttatttgaaa caagagaaca agccaaacgt
    48121 tcagtgatgg ctggtttgat ttacaacgaa aaaaacgagc ggtttgataa accgggagaa
    48181 aaaattccag tgagtagtga attaagagtc aaaggtaaaa aacttcctta tgtttctcga
    48241 gggggattaa aattagaaaa agcactcaaa gcatttgatc tatctgttga aggaaaaaca
    48301 ttgttagata ttggtgcatc cactggcgga tttactgatg cagctttgca aaatggcgca
    48361 aaaatgagtt atgctttaga cgttggctac aatcagctag cttggaaaat aagacaagat
    48421 cctagagtcg tcgtaatgga aagagtgaat tttcgttatg caaaacccga agactttacc
    48481 ttaggaattc cagaagttgc tgttatcgat gtttctttta tttcactaaa actgatgctg
    48541 cctccattac atgctatttt aaaagaagag ggagaagtga ttgctttaat caaaccccaa
    48601 ttcgaagcag gcagagaagc tgtagggaaa aacggaattg ttcgtgaccc tcagacccat
    48661 caaaaagttt tagaagacat tttgtcattt gcggcacaag gtggatatga tgtgctggaa
    48721 ttaagttatt ctccgatcac ggggggagaa ggaaatatcg agttcttggc tcacttgagg
    48781 aaagtacccg aaagcggaac aatcaattca gctattaata tggcagaagt ggtttcaaat
    48841 gcacacgaac agtttgacca taaataaatt gtaactgtga aaatcaagtg agaagtatgc
    48901 ggattcttct ttattatacc aaggaaataa gctatcattg attaagaaga atatgctaag
    48961 gagcgaagac gtaatgagaa aaaaagatcg gcatcgtttg attactcgat tgttgagtga
    49021 gcaagatatc cgaaagcagg aagaatttgt tgaaatcctg aaaagcaaag gaatatctgt
    49081 tactcaagct acgatttcca gagatataaa ggaactgaaa ctgatcaagg taccggcttc
    49141 agatggcggc taccgttata gcataccagc tgaaacaaac gaagatgtaa atactaaatt
    49201 ggaaaaacta ttaaaagacg ctttcgtggc agttgaccaa atggaaaaat ttgttatttt
    49261 gaaaacattg ccgggaaatg cttcggcagc agcaaatttg attgataaac gttacaagaa
    49321 agaattattt tcgattatca atgatgacga taatgttttg atgattacac gaactgaaga
    49381 ccaggcagtt ttattgaaga aagacttcct gcattacctt taagttgagg agcgaatgtg
    49441 atgctacaag aaatcagtat tactaacttt gcgattattc cggaattgcg gctttctttt
    49501 cacgaaggaa tgaccgcgct gaccggagag actggtgcgg gtaaatccat tattatcgat
    49561 gctttaggtc tgttagcagg aggaagaggt tcaagcgatt atatacgtca aggagcagaa
    49621 aaatgtgtgc ttgaagggtt gtttgaacta ccaaaacaag aaggattctc tgaactgatg
    49681 gttgaattag ggatcgaaac agatgaggac aatctgattg ttcgacggga tatgtcctta
    49741 actggaaaaa atgtttgtcg agtgaatggt catattatca cattagctaa tctgagaaga
    49801 atcggcagtt atttagtaga tatccaagga caaaacgaac atcaggaact gctccagcct
    49861 gaatcacatc ttgctttatt ggatcgtttt ggagatgtag ctttccaaca gaagaaaaag
    49921 agttatcagc aagaatatat ctcttatcgt gagctggaaa aaagggtacg aaaagtgcag
    49981 caaaatgaaa aatcctatgt tcagagaata gatatgctcc atttccagca agaagaaata
    50041 gctgctgctg atttgcaagt cggagaagaa gagaaattaa aagaagaaag agaaaaactt
    50101 agcaattatc aaaaaattgc tgatggatta gctgcaggtt acggagcact gacgaataat
    50161 gaacaaaaca gtgtagatgg tgttggcttg gccgtttcag aactgcaagg gatcgcccat
    50221 ctagatgtgg agtatgaagc aatttatgaa aatatccaaa gtgcttacta tttgctgcaa
    50281 gatgcaatcg gcgatatgag tcgtcagatt gatttattgg agttagatga gagtcgttta
    50341 gaagaagtta cgcaacgttt agaattaatc cgacagttga aacgtaaata cggcgaatcc
    50401 attgagtcaa tattggctta ctatgacgaa atcaccgaag agctagcttc ttctgatttt
    50461 tcagaaggtc aattggacaa aatgaaagag gaactagagc aaaaagagct tctacttcag
    50521 caacaagccg cagatcttca tgaagcgcgt aaaaaaatcg caaaagaatt ggaaaaatcc
    50581 attctgcatg aattgaaaag tctgtatatg gaaaacactg agtttgaggt acgtttctta
    50641 aaagaagaaa atagacaatt gaatcatgat ggatttgacc aaatcgaatt ttacattacg
    50701 accaatccag gagagccact gaagcctttg gtgaaagtcg cttctggtgg cgaactgtct
    50761 cggatgttgc ttgctttgaa aactattttt tcttctgagc aaggtgtgac aagtattatt
    50821 tttgatgaag tggatacagg tgtgagcgga agagttgcac aggccatcgc agataaaatt
    50881 tctcaaatat cgaaatactc tcaagttttg tgcatcactc atctgcctca agttgctgca
    50941 gtcgccgact atcaatatta tattgtgaaa gcagtgattg atggacgtac gcaaacttct
    51001 gtttcagagt tgaaaacaaa agaaagagaa gaagaaattg cacgtatgct ggctggaagt
    51061 gagattacaa aactcacact cgaacatgca aaagaactat tgaaactcgc aaaaaggacc
    51121 tgagccacct cgcagtggtt caggtccttt tcagcagctg atagctatat tttattttta
    51181 catgacactc attacagcag tcaatacaac agatccaatc gttaaaatca gcattgtcca
    51241 aatgacaaat tttgttactt tagcaaaagt acttgtttgt tttttttcac gcattgtttt
    51301 caacctcgtc attaattagt tgattcaagt gtacaatctt ttttccattt ccacaagagg
    51361 gcttttttat aaaaacttcc ctttttttaa ataataggta gttacgatac aaataaataa
    51421 tgttaataga actagccata taaaggcatc atccctttca gcaataggta acttaatgtt
    51481 cattccatag atactgccga cgattgtcgg tatagttaaa ataatcgtta aagaagttaa
    51541 aattttcata atattattca gattattcga tacgattgct gaaaaagtct gattcatttg
    51601 ttctactagt tttaattgaa tgcgagaagt agtcattgcc tgctctgttt ccaccaatac
    51661 atcacgtaaa tgatttttca aagcatgatt ttcttggaaa agtttatttt gcgctagttc
    51721 tttcaatgca tctaaattag cagaagtcgc ttctctgaaa taaacgaggc ttttttggat
    51781 atccatgatt tgataaagtt gtttgttttc agtggatact tgaagtttgg cttccaaagt
    51841 atcgcattga gattgaagcg tagctaaata actgttgtaa gagagtgtga cctgccaaat
    51901 gaattgaaag aagatattga gattaggcga aatatcactt tttggaaagg tctggctaaa
    51961 gactttcttt aaaaacagtg gttcatgatt cgtaattgtg ataagcttct ttttgggcgt
    52021 caaaataata gagagcgggt atgtatccac ttgaaaataa ccgctaggac ttgttttagc
    52081 aaaaggatat tgtaacaata ataacgctgg ctttgtcaac tttgattgat tgaattcttc
    52141 tgtacgggaa ttttctgcat cgtccaatat ggaagtgatg taatctgacg gaatttcaaa
    52201 ttgatgtgtc agctgatgaa tctcttcttg attaggacgt tctacacaat accagatcgt
    52261 ttggtcagtt tcctttccgg tattacgcag tttctcatta tctaatgtaa aatatttgat
    52321 catgattttc ctcctgttgc ctgtttcttt tattataacg gatagggaat tggaatacat
    52381 ggaatgtgtt tgtaagatat agaatataac tgcataaatt taaataaaat gaaacagttg
    52441 tgtaaagtat atacagtata cttgaagcaa cgataaaaaa ggagtttacg ttcatgggca
    52501 cattcacacc acttatcttg ttttggtata tttacccggt aatcgttttg tttggttgcc
    52561 aatttcttgt aaaattattt tcgttaaatc gtcgttttaa aataaaagcc cctgatttgg
    52621 cgattccttt tttgtggggc ggtatgcatg cattgtctag aaatacaggg acaatttcta
    52681 tcttaccgtt tttctttatt tctgttttgc taatgggaat ttgtattgct gttttccaag
    52741 catattatta tgaagaaatt atttatccgc gttatttcaa gatgacttgg cgtttggttt
    52801 ttctgttgac aatcgtcttg tacgctgtct tgatcattgt gaacatactt tcttacctat
    52861 gaaaaaaata gaaaagctgt aaaaagtctt ttaaaaggct ttttgcagct tgatttttaa
    52921 aaaaatgttt atgaataaaa aaagttgttc ccggatttcc cagaaaaggt ggtagaaagt
    52981 gggggattgt ggtagactaa ttttagttag tggaggaatg gggggctttc aatgttgatg
    53041 ggtgaattcc aacataatat tgatgcaaaa ggacgtttaa tcgtgccctc aaaacttcgt
    53101 gaagaacttg gcgaaaagtt tgttctgaca cgcggattgg atggctgtct tttcggctat
    53161 ccgatgagcg aatgggaaaa tctggaagct aaactgaacg aaatgccgtt ggctaaaaaa
    53221 gacgcacgta cttttgtgcg attcttctat tcggctgcca ctgaatgcga acttgataaa
    53281 caaggacgaa tcaatatacc cagcacgtta agaaattatg cagctttgac aaaaggttgc
    53341 gtagtcatcg gtgtatctaa ccgaattgaa atttgggatg agacacgatg gcaagatttt
    53401 tctgcagcag cagaagagaa ctttgacgaa attgctgaaa gtatgattga tttcggacta
    53461 taagaatagg aagaggctga ctaatgactg aaacatttca acactataca gtcatgttga
    53521 aagaaacggt agatggattg aacatcaaac cggatggcgt ttatgtagac tgtacactcg
    53581 gcggtgccgg acatagtgaa tatttattaa gccaactaag ttctaaaggg catctgtatg
    53641 cgtttgacca agatcaaaaa gcaatcgatc acgccaagct gcgtttagct tcttatgtcg
    53701 aacaaggtat ggtcacattt atcaaagcga acttcagaga attagaagaa cgcttgagtg
    53761 agcatgtaga taaggtagac ggcattttgt atgatctagg ggtttcctct cctcagctag
    53821 atgaagcaga acgtggattt agttatcatc aagatgcacc gttggatatg cgcatggatc
    53881 agtcagctcc tttatccgct tatcacgtaa ttaatgaata ttcctaccat gaattagtaa
    53941 agattttctt ccgctatggc gaggagaaat tttccaaaca aattgctcgt gaaatcgaac
    54001 gagtacgaaa aacaaaacca atccaaacaa ctggtgagct tgtcgaaatc attaaacaag
    54061 tcatcccagc tcccgcaaga agaaaaggcg ggcatcctgc aaaaagaatt tttcaagctg
    54121 tgcggattgc agtaaatgac gaactcggtg ccgaagaggc ctctttagaa caggcaatcc
    54181 ggttattgaa agtcaatgga agaatcagcg taatcacatt ccattcattg gaagacagaa
    54241 ttgtcaaaag tatgtttaaa gaatatagta cggtacaaga tctaccaccg ggacttcctg
    54301 tcatacctga cgaattccaa ccagaactga aagtcattac tcgtaaacca atccttcctt
    54361 cagaggaaga gctaacagaa aacaatcgat cacgcagtgc aaagttacgt attgcagaaa
    54421 aaataagaat gaaagaggag tagaatgatg gcagaattaa agaaaatgca agattacgaa
    54481 tatgatttac ctgaattggt ggatcagccg gaacaacaaa tgcctgattc ttcttcgaat
    54541 caagaaatca ttcttccaca atcaccggca aaacgattaa agcagatttc tgctttggaa
    54601 aaaattgctg tggcaagcgt ggcgattgct attgttgctt tatgtgtttt aacagttatg
    54661 ctgcgcacaa atatcagcgg agctgaaaaa gaaatcacgt caatacaaac agaaatcaat
    54721 gataaaaagc aagaaaaaac gagtttagaa caagaaaaaa acgaactttc tcgtactgaa
    54781 cggattaaac aaatcgcaga agaaaaagga ttgtctatta atgacgacaa tttaaggaaa
    54841 gtgaagtaaa tgagtcttaa aaataaaatt cggcggttta tggaaaagaa aaatctaaat
    54901 ccgatgaaca atcgcaaaaa agtaggtatc attttatttg ctacgagtat tggattgttc
    54961 tttttatttg ccgtccgttt ctcgtatatc gttattggag gtcatgttgc tggtacctca
    55021 ttagctgaaa aaacaaaaca gttgtaccaa ggaagtgaag tagtaaaagc aaaaagagga
    55081 acgatttatg atcgtaacgg tgtggcgctc gcagaagatg cctcctctta ttcaattaaa
    55141 gcgatactct caaaaacata tacctctggg gataaaaaac tgtatgttga agaaaagaat
    55201 tttgataaaa tagcagaaat cctgcataag aatctttcta tagacaaaaa agatgcactg
    55261 aatattttag aggacggcgc aaaaaaagag ctgtatcaag ttgaatttgg tagctatggg
    55321 aaaaatatct cccaagaaac caagcaaaac atcgaagcgg acatgaaaaa agaaggtgtc
    55381 gctggtcttt attttgtcga ccatcaggct cggatgtatc caaatggcgt cttttcttct
    55441 cattttattg gatacgcagt tccagacaaa gatgagaatg gcttagttgg taaactaggt
    55501 ttggagtctg cttacaatga tatcttgagc ggtaaagatg gtaagattat ttatcaaaaa
    55561 gataattttc aaaatcctct gccaggtaca gtggcagaag aagaaaaagc tgtcgacgga
    55621 caagatatct acacgacttt agacagcaga ctacaaagtt atttagaaac actgatggat
    55681 caagtaaatg aagaatatca gccagaagaa ttgacagcag tcctgatgaa agcaaaaacc
    55741 ggagaaatct tggcaatggg acaacgacct actttcaatc ctgaaacaat ggaaggattg
    55801 actggaaaag atgctatctg gcgtaatttc ttggtacaag atagttatga accaggatca
    55861 accatgaaag ttttcactac tgcagctgct atcgaagaag gagaattcaa cgagaatgaa
    55921 accttccaat ctggaaaaat ccaagtagaa gatgctacga tcaatgacca tgactttggt
    55981 gaaaaaggtg tgctgaccat gcgtcaagct ctttcatggt caagtaacgt cgggatggtc
    56041 atactggaac agcgattagg cggacgatgg tataactatt tacaaaaact gggatttgga
    56101 caaagtactc attctggatt agatgatgaa gtaaacggtg ctttacctac attaaatatt
    56161 gtcgaccgag caatgagtgc ttatggacaa gcggtaggtg tgacgaattt ccaaatgatg
    56221 aaagctttca cttctattgc gaataacggt acaatgattc aacctcgata tatcagcaaa
    56281 gtggtggatc cccaaactgg agaagagcga acaactcaaa cagaagttct agggcaacct
    56341 ttttcaaaag aaacaacaga aaaagtccgt gaatatatga gagatgttgt cgaaagcgaa
    56401 aactacggta gtgcttatgg tgtatatagt gtacctggat acaatgtatc ggcaaaaacc
    56461 ggaacagccc aaatcgcttc tgatacaggg ggatatcaaa caggagatac agcctacctg
    56521 tattcgattg tggagatggt tccttctgaa gatccagact atgtcttgta tctaacaatg
    56581 aaacacccta agacatatga tcgaatggca ttggctaaaa tcgcaaatcc attgatgaaa
    56641 cgggcaatgg attttaaaga aactgaagaa gattcagata cagaaacaaa aacagaaaaa
    56701 gtatccgtag ccgattatcg taatcttgaa gcggacgttg cagcagctga tgctcaaaaa
    56761 agtggactgc aacctgtagt gatcggtaat ggtaaaaaag tacaaaaaca atctactgcc
    56821 aacggtgatc agttgatctc tggagaaaaa ctgatcctat atactggtgg tgataagctg
    56881 atgcctgacg taacaggatg gtcaaaagca gatatcatga agctggggaa aatcctcggt
    56941 attgaagtta gttttgatgg agatggttat tgtgtcaaac aagaacttgc tccatatgaa
    57001 aaaatcactg aagacaaact tagttttact ttagaagaat aagaagagaa ataggagaga
    57061 aaagagcatg gaatggacac aagcattgat tcccattgtc agcagttgtg caatgacaat
    57121 tgccgcaatg ccgctattta ttggttattt tcaaatgaaa aagcaaggtc aggcgattcg
    57181 tgaggaggga ccaaagtggc acaatagcaa agccggaact cctacaatgg gcggcttagt
    57241 gtttctgatc ggctctattt tgacaggaat ttgggttgga gcttggcaaa aacagctaac
    57301 tcctactctt ttcatcttgc tttttgtatt agcgttatac ggagtaatcg gctttcttga
    57361 tgattttata aaaatattca agaaaagaaa catgggactg aactcgaagc agaaattgat
    57421 tggacagatt ctagggggaa tcatctttta tcttgtttac cgttctgaag gttatccagg
    57481 tgtattgaat ttcttcggac tagatctacc tttaggaatc atttatggta ttttcgctct
    57541 cttctggctt gttggatttt caaatgcagt aaatctgaca gatggcatcg atggattagt
    57601 tgctggctta ggaagtatct cttttgcagc ttacggtcta attgcttggc atcagcatca
    57661 gtatgatgta ttaatcattt gcttaagtgt tttaggaggt ttactgggtt tcttcgtcta
    57721 caacagaaaa cctgccaaga tttttatggg agatgtcggt tcattagcgt tgggcggact
    57781 tttagcggca atttctataa tgctaaacca agaatggaca ttgcttttga ttggtttgat
    57841 ctatgttatg gaaacagcaa gtgtgatgct tcaagttact tcctttaagc ttacaggaaa
    57901 acgaattttt aaaatgtctc caattcatca tcactttgaa atgtgcggat ggtcggagtg
    57961 gaagattgat acagtttttt ggttgatcag cattgtgact tcgttgatca cattatggat
    58021 cgtttggtag gagatgacgg gaaatgaaaa aaatatcaac atatgaaaat aagaaagtgc
    58081 ttgtcttagg actcgcaaaa agcggagtaa gtgcggccaa attattacat gaattgggcg
    58141 cacttgtcac cgttaatgat ggaaaacctt ttgacgaaaa tccggaagca caagagctgc
    58201 tttctttagg gataaaagta ataacaggta gtcatccaat cgaattgttg gatgaagaat
    58261 tttcattgat ggtcaaaaat ccagggattc cctacagtca tcctttagta gcaaaagccc
    58321 aagaaatggg tattccggta attacggaag ttgagctagc ttacgaagta gcggaatgcc
    58381 caattattgg gattacagga acgaacggaa aaacaacgac aacaacgatg acaggactac
    58441 tactgaatgc aggagcagat caaggaattg cacgtttagc gggtaatatt gggtatccag
    58501 caagtggtgt agcgcaagaa gcaaaatcag aagataaaat tgtgatggaa ttgtctagct
    58561 tccaactcat gggaatcaca gatttccgcc ctcatatagc ggtgattacc aatatctatg
    58621 aagcacatat tgattatcat ggtacaagaa aagaatatgt caaagctaaa tggaaccttc
    58681 aaaaaaacat gacagaaaaa gattatctga tccttaactg gaatcagtca gaactacaag
    58741 aattagcgca aagaacaaaa gcacgcgtac tgcctttctc gacaaaagaa gtgttggaag
    58801 acggtgtgta tgctgacgat tactcaatat actataaaaa agaaaaaata atggaaatat
    58861 ctgaacttgg cgtaccaggt aagcataatg tagaaaatgc actagctgct atttctgtag
    58921 caaaattgta tggtatatca aatgaagcta tacgagaaac gttgcatttt ttccatggcg
    58981 taccacatcg tacgcaatat gtaggagaaa tccaaggcag aaaattttat aatgattcaa
    59041 aagcaaccaa tattttagcg actaaaatgg ctctaagtgg atttgaaacg tctaaggtcg
    59101 tgttattggc tggcggatta gacagaggaa atacatttga tgaattaatt ccttcattaa
    59161 aaggaatcaa agcaatggtc gtatttggcc aaacaaaaga aaagttgatg gatgctggaa
    59221 aaaaagcagg catcgagaca atagtaacag ccgattctgt agaacaagca gttcctttag
    59281 cattagaaaa cagtacagat ggtgatgtag ttttattatc accagcgaat gccagctggg
    59341 atcaataccc aaattttgaa actagaggaa acagattcat ggaagctgtg aaccgattaa
    59401 aatagaaaaa gtagaagcga gcgataaaaa atgaaaattt tggttacagg cggaggaaca
    59461 gggggacata tttatccagc tcttgccttt gtcaattatg taaagactca agaaccgaat
    59521 gctgaattca tgtatgtggg agcaaaacga ggtctagaaa acaaaatcgt ccctgataca
    59581 ggaatgcctt tccatacctt ggagattcaa ggatttaaac gaaaaatttc catgcataac
    59641 atcaagacgg tccgattgtt tcttaagagt atccgtcaag caaaaaaaat cctcagtgat
    59701 tttcaaccag atatcgttat cggtactgga ggatatgttt ccggagcggt agtgtacgct
    59761 gcagccaaaa tgggtattcc aacagttatc catgaacaaa acagtgtacc aggtgttacc
    59821 aataaatttt taagccgata tgtggatcgg atcgctcttt catttaaaga tgcggcttcc
    59881 ttttttcctg aaaacaaagc agttcttgta ggtaatcctc gagcacaaga agtagcagat
    59941 actaaaaaat cagatatttt gaaaacattt gatttggatc cagaaaagaa aacagtcttg
    60001 atttttggcg gtagtcaagg agcacttaaa atcaatcaag cagtatcagt ttttttaagt
    60061 gaatatattc cttcttctta ccaagttttg tatgcttcag gagatagata ttatgaagag
    60121 atcctaaata aaataaatcc tgaaatacaa aatattagtg tccagcctta tatcaaaaat
    60181 atggcagagg tcatggcttc cagtgatctg ctggtggggc gtgctggtgc aacttcgatc
    60241 gcagaattca cagctctggg gttaccggca gtgttggttc ccagcccata tgttacgaat
    60301 gatcatcaga ctaaaaatgc aatgagtctt gttcacgcag gagcagcaaa aatgattgca
    60361 gataatgaac taacaggtga atcactaagt cagacagtca acgaaatcat gggtgatgaa
    60421 gaattgcaaa agcaaatgtg ccgagcttct aaagaacaag gaatccctga tgcttcaaaa
    60481 cgtctttatg acttagtaaa acaaataatc aaaaaataaa ggcggtggtc ttagtgagta
    60541 agaaaaaaga accaaaaaaa ttgtctcatc cgtcttctga tgaaaacaat ttgactcctt
    60601 ggcaaaaggc taaccgaaaa tatctggcag aacaagagat gaaaaaccaa gagccaaaaa
    60661 aagaagacat aacattagat acaccccttt cagattctga agaagaaaaa acggttcaag
    60721 aggaacagca agaagaaagc cagtccgaga aaaaaatcga attttttgaa gagatcaagc
    60781 aaaagaaaaa cggtggacca tataacggtt cttttttgaa ccggctgccc aatttaaaaa
    60841 gtcaacggca caaagtacta tttcgtcggt tagctttgat catcactgta ttgggtatac
    60901 cgctgatttt tttgatctat tatgtttctc cttatagcaa attacaagca gttgcagtat
    60961 caggcaataa gacagtcaac agccaagaaa tcatctctga tacaaaattg tcattaggag
    61021 aaaatgtttg gggacagtat tttcatcgat ctacttatat cgatcgcctt aaaaaagcac
    61081 agccaagaat cgaaacagca aatatacatt tcaaagggat gaatgaattc gatttggatg
    61141 taacagaata taaagagatt gctttgatcg caaaaaacaa tcaatactac ccagtgattg
    61201 aaaatggaac agtattagat gaaaaagtag caaatcctac aaaaaatctc cctatcttgg
    61261 aagagttcaa agactctgca aaaatcaaag aattaacaaa gcaatacaat cagttatcat
    61321 cagaattaca aaaagcaatt tctgaaatca agtacacgcc aagagcaagc aataaaaatc
    61381 tgatccaatt gaatatgaat gacggcaatc aagttattgt aaatatcaat aatcttgcga
    61441 accaaatgaa atattattcg caagtagcca aagatatgga tgaaaaaggt gtgatcgaca
    61501 tggaagttgg tatcttctcg catccttacg gtagcactca agaaacggaa gaatcgggcc
    61561 aatcggaaga aacaactgaa agtgaagaaa atgcggatca aacaaacgaa tcaagtgaag
    61621 aggcagaaac gcaaaaagca caaacacctg gatcgtctag cacaaacgga tcatctgatt
    61681 caaatgattc aagtgaaaca agtcaaatag aagataatcc aagtacaact ccgtcaagtt
    61741 taaatgaaaa ttgataaaac aactttaaaa agtaagaatg tactttctaa aaaccagtaa
    61801 tttatggtag aattggaagc agtgaatata tctattaata agtatatttt aaaacggacg
    61861 ctagttgttc gcttgtatat ggaatagtaa gtaggaggga atcccattca tggcaaaaac
    61921 aggaatgtat gtaggccttg atattggaac gacatctgtt aaagttgtcg tggctgaata
    61981 tatcgacagc caaatgaata ttattggagt aggaaacgca aaatcagaag ggattaaccg
    62041 aggcattatc gttgatatcg ataaaacggt ccaagcgata caacgagcag tacgacaagc
    62101 agaagaaaaa gcaggtatcc agatcaaagg cgtaagtgtc ggtttgccag caaacatgct
    62161 tgaagtagaa aattgccagg gaatgattgc agttaacgga gattcaaagg aaatcaccga
    62221 tgaagatgtc agaaatgtgg cttcagctgc ccttgttcgt tcaatacctc ctgaacgtca
    62281 aatcgtgtca atattgccac aagattttac tgtcgatggg ttcgagggaa tcaaagatcc
    62341 tcgtggaatg atcggcgtaa gattagaaat gtacggtctg ttgtttacag gacctaaaac
    62401 gatcatccat aacatacgta aatgcgtgga aaatgccgga ttaatcgtca acgaaatggt
    62461 gattactcct ttagcgctga ctgaatcaat tctttctgac ggagaaaaag attttggtac
    62521 gatcgtcatt gatatgggtg gcggccaaac aacaacggct gttatgcatg ataagcaatt
    62581 gaagtttacc aatcttgacc aagaaggtgg cgaatttgtc actaaagata tctctattgt
    62641 attaaatacg tcatttaaca atgcagaagc gttgaaaata aactacgggg atgcataccc
    62701 agaacggaca tcaccagatg aagaatttcc agtagatgtg attggtcagt cagaaccagt
    62761 gaaagtcgat gaacgctact tgtcagaaat tatttcagct cgaatggaac agattttcaa
    62821 caaagcaaaa gatgcattgg accaaatcga agcattagag ttgccaggtg gcgtcgtatt
    62881 gacaggcgga gctgctagtc ttcctggtgt tgtggacttg gcacaagaaa tcttcggcgt
    62941 aaatgtaaaa ttgtatgttc caaatcacat ggggctgaga aacccggtat ttacaaatgt
    63001 aataagtatc gtggattatt cagcaaactt gagtgaagtg tatcaattgg caaaaattgc
    63061 tgttacgggt gaaacctcag cagctcgtca aatggtagtt gaacaagaaa caacaaacac
    63121 atatgagagc tatgaagcac ctgaagaaac aatctacgat gagccggaac caaaagagtc
    63181 cggagaaaat gtaaaaaata aaatcaaggg tttctttaca aatatttttg attaattcat
    63241 cctgggagga ataagatatg gaattctcaa ttgataataa tatcaatgac ggcgcagtaa
    63301 tcaaagtcat cggagtcggt ggtggcggtg gaaatgctgt aaaccgaatg attgaagaaa
    63361 acgttaaagg tgttgaattc attacagcca acacagacgt gcaagcatta aaaaattcaa
    63421 aagctgaaac agttatccaa ttaggcccca aatatactcg tggtttaggt gctggttctc
    63481 agccagaagt tggacaaaaa gcggcagaag aaagtgaaca atccttacgc gaagctttag
    63541 acggtgcaga tatgattttc atcactgcag gaatgggtgg aggaaccggt acaggtgctg
    63601 caccaatcgt tgcaggtatc gcgaaagaat tgggcgcatt gactgttggt gtcgtgacac
    63661 gtccattcac ttttgaagga ccgaaacgtg gccgttttgc tgcagaaggt atcgctcgtt
    63721 tgaaagaaaa tgtggataca ttattaatta tctcaaataa ccgtttatta gaagttgtag
    63781 ataaaaagac accgatgctt gaagctttcc gtgaagcaga taatgtttta cgacaaggtg
    63841 tacaaggtat ctctgatttg atcacagcgc caggttacgt taaccttgac tttgctgacg
    63901 tgaaaacagt gatggaaaac caaggtacag cattgatggg gatcggtgtg gctagtggag
    63961 aagatcgcgt agtagaagca acgaaaaaag caatctcctc tccattgctt gaaacatcta
    64021 ttgatggagc tgaacaagta cttcttaaca tcacaggcgg tttggatatg acattgttcg
    64081 aagcacaaga tgcttcagat atcgtagcaa atgcagcaac tggcgatgta aatatcattc
    64141 taggtacatc tatcaatgaa gaaatgggcg atgaaatccg tgtgacggtc atcgcaacag
    64201 gtatcgatga atcaaaaaag gaacgcaagt ctagccgacc agctagacaa gcacaaatgc
    64261 aatcacctgc tcaaaagact gtattagata tggatcaagc taaaccaact tcttcagaag
    64321 aagaaaacag ttttggcgat tgggatatca gacgtgaaca aaatgttcgt cctagagttg
    64381 atgattccaa ttttgacaat atcgaaaaaa aagaatttga tacattcaac cgcgaagaaa
    64441 caaaatctca tggcgatgat gaattaaata cgccaccgtt tttccgtcgt aaaaaataaa
    64501 ggagaggaag agcacatgat tgctgacaac ttgcagaaaa tcaagcaaga aattcaggat
    64561 tcgtgtgctc ttgttcatcg tgatccgaaa gaagttacat tagtagctgt aacaaaatct
    64621 gttgaatccg ctcaagctga agaattggtt agagctggcg ttatgaattg tgcagaaaac
    64681 cgagtagata aattattaga aaagaaacaa gatttatttg attttcctga aataaagtgg
    64741 catttaattg gtaatttaca gcgcagaaag gtaaaattga ttgtaaatga aattgattac
    64801 tttcatgcgt tggacagttt aagattagca gaagaaatcc agaagcggtt agagaaagaa
    64861 ttatcctgtt ttattgaagt aaatgtttct ggtgaggaat caaaacatgg aatttcacca
    64921 gatgagcttt taccgtttgt ggaatcttta gctgcctgca caaaaatcaa ggttgttggg
    64981 ttgatgacta tggcgcctaa agaagcttct gatgagcaaa tccataaagt gtttggtacg
    65041 ctgaaagaat tgcgtgatct tgttcaagca aaaggttatg cccatgcacc atgtagtgaa
    65101 ttaagtatgg ggatgagtca agactttcaa attgcgattg aagaaggtgc aacatttatc
    65161 cgtgtaggaa cagcgctatt caaagacgaa taaaggagga acaaaaatgc cgcttttaaa
    65221 cagaggagca attgctaatt ttttcggatt agctgatgag gaagagtacg aataccaaga
    65281 ttatcctgaa acaacaaaaa aggctgtaca gccaactaaa aaaaatacaa cacagaatca
    65341 atcaactcat tctgtatctg ctgcgaatac tcatgaaaaa caaaaaacgg cgacttcacg
    65401 tcctgtaacg catgaaagta ttcagccggc tgaaaagaaa aaagtaattg aactacatca
    65461 atcacctaca aaaactgtag cgaaagaaag aacagcttca agaacctcta cacaacctaa
    65521 agcaaatctt gctggaaaaa tcactgtaat ggaaccaaga gcttattctg aagctatggc
    65581 gattgctaaa cgaatcattg ctggagaagc aaccttagtg aattttcatt tagtagaaga
    65641 aaaacaagcg agaagaatcg ttgattttct aacaggaaca gtttatgcac tagatggaga
    65701 tatcaaacgt gttggagatg agatttttct ttgtacaccg actgggatgg agattgacag
    65761 ttcaaccgct caatcttttg cagatgcgaa tttgtttgat ctgtaagagg aggaactacg
    65821 atttatacta ttgcactatt acttcattta cttcaacggg cagcctattt ctatagcatt
    65881 atcttgatgg tatatgcatt gctctcttgg tttccaggcg ggtatcaatc agggctagga
    65941 cgattattag ctaagatctg cgaaccgtat atcagtttgt tcgatcgtct gcctttgagg
    66001 attggaccct tggatttcac aattttaggg gcaatcctga tcttgaactt agccgtacaa
    66061 ggtttagcac agatcgttat atggataact gtccatttag tttattgatt gaagcaacaa
    66121 aatctttatt ctcagaatag ataggagggg aaaattttgg atgcaaatgt ataccagcac
    66181 tttcgggcag atgaacgtac atttatcgat tcggtaggcg attggattga acaggttcaa
    66241 ggacaatatg caccctatct gaccgaattt cttgacccaa gacaatccta tattttagaa
    66301 acactgattc gacaaagcag tgatttgcgt ttcatgtttt atggcggata tgaacaggca
    66361 gaaagaaaaa gatgtttgat tttccccgat tattacgaac ctgtagagga agattttgaa
    66421 acagcattgt atgaaatcca ttatcctagc aaatttgcca gcctaagtca tgggaaaatc
    66481 cttggaactt tagtgggaac ggggatcaaa cgctcttact ttggcgatat catctcggac
    66541 ggtgatcgct ggcaagtatt tattgcaaat gaggtggatc attttgttgc tgcacaagta
    66601 acaaagattg gaaaggttgc tgtacgcttg gaaccaatca aatacacaga aatgattatg
    66661 ccgaaagata gctggacaca agaaagagga acagttagtt ccatgcgact agacagtgtg
    66721 atctctgaga tttataatat ttccagacag cggtccaagc agctgatcga agctggtaaa
    66781 gtaaaagtga actggacaga aaatacaaga cctgattttg tacttgaact attagatatt
    66841 atttcagtca gaggttatgg cagagtccag atccaagaac ttgaggggaa gacgaaaaaa
    66901 gaaaagtaca gggtactgtt cggtgtatta cgtaaataaa ataaaaaggt ggtttactca
    66961 tggctttaac tccattagat atccaaaata agacattccc gacaaaaatg cggggatata
    67021 atcaagacga agtagatgat ttcttagatt tagttgttcg tgactatgag gaattgactc
    67081 aaagaaatcg agaattagaa aaagcagtca aacattctga agaaaaactt gagtacttca
    67141 atgaattaaa agatgctttg aaccaatcaa tcatcgtagc tcaagatacg gctgataaag
    67201 taaaaacaag tgcaagcaag gaatccgaag tgatcgttac ttctgcacaa aacaaagcag
    67261 atgagttagt tgcgaacgct gaaaaacgtg cacatcaatt aacaactgat gcagaagaaa
    67321 aagcaagaaa aatcttgaca gatgcaacag aaaaagcaag acagcttgcg acagaaactg
    67381 aagatcttaa gaaaaaaaca cgagtattcc atcaacgtat cagcttgatg cttgagtctc
    67441 aattagaaca agtgaaaagt ccggaatggg atgaaatttt acaaccattc tccagctacg
    67501 tgactgacag ccatgaagtg atcaaagaag ttttagcaaa acagcttgac aatgaaaatg
    67561 atactgatgt aaactcagac acaggagtca tagaagcacc tatgacggca tttgatacac
    67621 aggctattga tatctcgatc gtgcctggtg aaaatgaaaa cgagtagaac agaaaagcac
    67681 tttgttattc ttaaagcgag ccgatgagag tggaagttcg gtaagacact gcatggtgaa
    67741 agatcctcta cttgagacct gcttttccga aattagagta agaagcagcg gctgacctcg
    67801 ttattggtca cagagagttt gcactagata gatgcaacta aatttgggtg gtaccacggc
    67861 atgttcgtcc cttgcggctg aacatgcttt tttgtgtccg gaaaacaaat aaaggagtgt
    67921 atggttgaat gaaaatgaaa gaaaccttgc aattaggaaa aacaaaattc cctatgcgtg
    67981 gtaatttgcc aaaccgagaa ggcgagtggc aaaaagaatg ggaagaaaat cgaatctacc
    68041 aaaaaagaca agagttgaac gaaggaaaac ctagctttgt tcttcatgat ggacctccat
    68101 atgccaatgg taatatccat ttaggacatt cattgaataa aatcagtaaa gacattatca
    68161 ttcgttcaaa atcaatgtct ggctatcgtg caccatatgt tccaggatgg gatacacatg
    68221 gattgccgat tgaacaagta ttggctaata aaggcgtgaa acgtaaagaa atgtccatgg
    68281 cagaatactt ggaaaaatgt cgtgaatatg cactttccca agtagataag caacgagcag
    68341 atttcaaacg cctaggagtt gcaggagatt gggaacatcc ttatgttaca ttagatcctt
    68401 cttatgaagc tgcacaagta cgcgtatttg gtaaaatggc tgaaaaaggc tatatttaca
    68461 aaggattgaa accaatttac tggtctccgt caagtgagtc ttctttagcg gaagctgaaa
    68521 ttgaatataa agatgtaaaa tctccatcta tttttgtagc tttcaatgtc aaagacggca
    68581 aagatattct tgatgaagat acagcttttg tcatttggac gacaacacca tggacattgc
    68641 cagcaaacca aggtattgct gtaaatccaa aatttacgta tgtattggtg gaagccgatg
    68701 gccgtaagtt tgtcgtagcg aaagatttgc tagaaacagt ccaatcagaa atcggttggg
    68761 aaaatgtaac agttctaaaa gaatttgctg gacaagaaat ggaatatatg actgcaagtc
    68821 atccattcta tgaacgtgaa tcacttgtga ttttaggcga tcatgttact ttagacgctg
    68881 gtactggatt agtccatact gctccaggac atggggaaga tgactatatc gctggtagca
    68941 aatataaact atctgtagtg tcacctgtag actcaaaagg attgtttaca gaagaagcac
    69001 ctggatttga aggtatcttt tatgacaaag caaatcctat gatcactgat ctattaaaag
    69061 aaaaaggagc tttgctgaag ctagatttct ttactcatag ttatcctcac gattggcgga
    69121 cgaaaaagcc tgtgatctat cgggcaactc cacaatggtt tgcatctatc gataaattcc
    69181 gtcagaatat tttggatgaa gtagaaaaag tagagtgggt tatcccatgg ggtaaaacac
    69241 gtttgtataa tatgatccgt gaccgcggcg attgggtaat ttcccgtcag cgtgcatggg
    69301 gagtaccact tccgattttc tatgctgaaa atggagaagc aatcatcaca ccagaaacaa
    69361 tcgaacatgt ggcaaaatta tttgctgaac atggatcaat tatctggttt gaaaaagaag
    69421 cgaaagaact tcttcctgaa ggatttaccc acccaggttc tcctaatggc gaatttacaa
    69481 aagaaaaaga tatcatggat gtctggtttg attctggttc ttctcatgaa gctgttttac
    69541 gtcagcgacc agaattatca ttcccagcag atatgtattt agaaggatca gaccaatatc
    69601 gtggctggtt caactcaagt atcacgacta gtgtggctat caatggtgta gcaccataca
    69661 aatcagttat ttctcaagga tttgctttag atggtgaagg ccgtaagatg agtaaatctt
    69721 taggaaacat cattgcacca gaaaaagtga tcaaacaatt tggtgcagat atccttcgct
    69781 tatgggtatc tagtgtagat tatgaagcag atgttcgtgt ttctatggat atcttaagtc
    69841 aagtggcaga agtatatcgt aaaatcagaa atacgatgcg tttcttatta gcgaatacag
    69901 aagatttcga tcctaaaaaa gatactgttt cgtacaatga tttacgttct gtagacaaat
    69961 atatgaccgt tcgattgaac caagtaatca aagaaatccg agaagaaggg tacgacaaat
    70021 ataacttcat gcatatttac cgtacagtta tgaacttctt gacggttgat ctatcgtcat
    70081 tctacctgga ttttgctaaa gatgtagttt atattgaagc agaagatgat tatcaacgtc
    70141 gttgcatgca aactgttttc tatcaaacag ctgtagcttt gacaaaatta ttgactccta
    70201 tcattccgca tacagcagaa gaaatctggt cattcctgaa agaagaagag gagtacgttc
    70261 agttaagcga gtttcctggt tatgaagagt ttgcaaacca agaagaattg atggatacct
    70321 ggactgcttt catggacttc cgtgataatg tcttaaaagc attggaagaa gcaagaaaca
    70381 gcaaattgat tggtaaatct ttggaagcaa aactaactgt ttatccaaaa caacaagttc
    70441 gtgaaatgtt aaaagctttg gatgcagata ttgcacaatt attgatcgtt tcaccagact
    70501 actttgaagt tatggcagca gatgaacaag tgccagataa cgctatggta tttgatgatg
    70561 ttgctattac agtggaaaaa gcagacgggg aaacttgcga tcgctgtcgc caagttcgta
    70621 aagatgtagg agtagatgaa aaacttcctc atctatgcgg tcgttgtgca aaaatcgttg
    70681 aagacttcta tcctgaagct gtagctgaag gattcgaaga aaagtaaaag ggaaaagggg
    70741 gtgtgacatg ttctttgtca cacccccttt tgttatcaat cgtttaattt tccacgttca
    70801 cgataccagt tatctggttg ccagatccat tcgaatccgt ctttttcaag tagttcgaaa
    70861 gctgctttag gtcccattga tccagctttg tagttgggaa aatccggttg tgtttggtcc
    70921 caagcttttc gaatgacatc cacgatatgc caagattgag cgacttcttc ccaatgagag
    70981 aagttcgttc catcaccatt caacgcatct aatagtaatt tttcgtaagc ttctgggcta
    71041 ttttcaacca tttctgcact atttctaaat tccaatttga ttggttccgt ttcaaatcct
    71101 tgccctactt ctttcccatt caaggataaa gaaaatcctt cagttggctg aatatagatc
    71161 gttagaacat taggtggaag tgtcgtatcg tcacatggtt catcgacaga tgttttgaat
    71221 acattaacag gaacttgttt gaatacgatg ttgatccgag tccctttttc agtcaaacgt
    71281 ttgcctgtac gtacatagaa aggaacacct gaccaacgga agttatcgat agtgaatttg
    71341 cctgcgacga atgtttcagt ctctgattga gcatccactt tgtcttcttc gcgatagccc
    71401 ttgaattgta caccatccaa ttcaccttga gcatattgcc cgcgaacgaa gttttctaac
    71461 acttcttctt cgttataaat gcggatagct ttcaatgctt tgatcttttc tgtacggatt
    71521 tctttttcgg aaaatgctac tggcggttcc attgcaagta atgctacaac ctgtaaaata
    71581 tgattttgca ccatgtcttt aagtgcgcca ctatgatcgt agtagccccc gcgatcttct
    71641 acaccgaggt tttcgccaaa agtaatttgg atattgtcga tatagcgatt gttccattga
    71701 gcctcaaaaa tattattggc aaaacggata gctgagatgt tttggatcat ttcttttcct
    71761 aaataatgat caatcctgaa aatatcttgt tctgggaata cttctctgat ttgctggttc
    71821 aattcaaaag cagactggta atcagaaccg aacggttttt ctatgatcaa acgattgaag
    71881 ccattttttg tcataatatt ttgtgatttc aaatgagaaa cgatcgttcc aaaaaattga
    71941 ggtgccatag ccaaataata gacacggtta ccgtttaatt gatagtgtcc atctaatcgg
    72001 tcagacaatt cttttaatgt ctgataatgc gttgaatcat ttacatcatg ggattgatag
    72061 taaaaatggc ttgcaaaggt agttgcttct tcatctgaag gattcaatgc acggattgtt
    72121 tctttgacaa cttcacggta gtgctcatcg ctccagggac ggcgagcggt tccgattaca
    72181 gcaaaatgct cgcctaaatt tccttttctg taaaggcgga acaaagacgg gtacaattta
    72241 cgctgggcta ggtcacctgt accgccgaat atcgtaaaca atacattttt ttcattagac
    72301 atgtggatac actcctttat attttatcga caaaaatcgt gctggccgcc ttaaagctga
    72361 cggggatttt ctttctatca ttttggatag tgatcggtcc ttcataagct gcgatgtcaa
    72421 cgatctcata ttcttcgttg atattcaggt caatagtgac taagtaatcc aacaattctt
    72481 tttcatcaag cactcgtgcg atacgcactt ttgtaccgac aggataggac tccaatgttt
    72541 gtcggcgttc ttcatggaca acttcctctt ttttaggaat cgatcctcca tgaggacaat
    72601 gttcaggatg attcaaatag ttttctaaat gatctgcaag atgttccgaa gtcacatgtt
    72661 ccaatacttc agcatcatca tggacttcat tccagctata gtctaaatgt tcaactaaaa
    72721 aaacttccca taagcgatgt ttgcgaatca gactgctagc tttttgcagt ccgcttgcag
    72781 tcaattgtac accttgataa ggggaatgtt ctacaagctc ttctttcaca agtttagaaa
    72841 tcatttcact gacagatgca gctgagacat ctagactaga tacgatttgt ttattgttga
    72901 ctttcgttgt atctccacct agttctaaaa tgattttcaa ataatcttct cgatttggtg
    72961 tcatattcga tcatcctttc atggtaaaaa accatcatta gtaagtgtaa caaaaaaaac
    73021 gttaattgac tatttttcta aataaaaaac aaaggatttt tagacgtttt tacaacaaaa
    73081 aacgaaagat gatagagaag actcattctt tcgtttataa aggatatata tatacattgg
    73141 gagggatcta tttttcagat aacaacttat atgaagtgtc tgcagattcg tcataaagag
    73201 tttgttcggg ataagaaaag ataagataaa aaccatactg atcacgtacg actaaacgat
    73261 attcatctat ttctaccaca gatgtgccaa gagactgacc gttgatcaaa aacgtgtaat
    73321 cagatgtaat ctgtaattgt tgtttttgct ggctatcact aaagaaccat gtaccttgat
    73381 actttgagag tccttctgac ttttccgctt gttttttctt tttcaagtaa ataaaacttg
    73441 agagaacggt aatgagtgaa ccaagaaaat agatcatttt ttttatcacc aaatcactcc
    73501 tttatgagaa aataatcgca tagacatatt ctttcttctt tatttcattg taccgaataa
    73561 aagctaaaat ttctctaaga ctttcgactg gtttttttac tgtttttcta caaattatga
    73621 taggatacat agtaggaaga aaggtgacat aatttgaagt atcagcaagt ggaaatagct
    73681 ttttttattg accgcccgaa tcggtttata gcacattgta tgaaaaaaga cggagaaata
    73741 attattgcgc atgtcaaaaa tacaggtaga ggaaaagagg tcctgatacc aggcgctgaa
    73801 gtggctattg tatttgctcc tggacctaaa cggaaaaccg cctatgatct gattgctgta
    73861 aaaaagaagg gtgactggtt taatatcgat agtcaattgc ctaatcggct ggcaattgat
    73921 ggtattttag acggcacgat ccttttacca ggattaactg gtccatttac ggtatacaag
    73981 cgtgaagtaa cttatcttca ctccaaattt gatatttatt tagagacggt ggtcggacaa
    74041 aaagcatttg ttgaagtcaa aggtatgaca cttgaaaata aagcgattgg tgcttttcct
    74101 gatgcgccca cactcagagg attgaaacat ataggagaat taacgtatgc cgcacaagat
    74161 ggttatgccg cttatgtctt gtttattgct cagttcgaac atcttcatct ggcaacgatc
    74221 catgaagaaa tgcaaccagc actagctgat atggttcgac atgcgcagca aagcggagtt
    74281 caaatcctcg cctataattg tcaagtatcg gcagatgagg taactgtcaa gcaggccatt
    74341 ccatttgatg tagatatccc ttttgaagat cctatacaat agaaagaagg taaaataatg
    74401 atcaacttag gtattatcgg aacaaattgg attactcatc aatttgtcca agcagcttta
    74461 agcacaaaaa aatatgaatt gacagcggta tattctagac atttggaaaa agcacaggaa
    74521 tttggttctc attatgaagg ggatatcgaa ttcgcaacag atttgaagac ttttttcaat
    74581 attactcata tggacaccat ttatattgct tctccgaata gtcttcactt tagtcaagct
    74641 aagcaagcaa ttttggcagg aaaaaatgtg atagttgaaa agccagcttt ttcgacgcca
    74701 aaagaaatgg atgaaatcat ccatttggca aatgaacaca atgtgtattt ttttgaagca
    74761 gcaagaaata tccatgaatc aggttttaaa aagattgccg attacttacc attaaaagac
    74821 gagataatcg gtgcaaattt cagctatatg aaatattctt cgcgctatga ccaagtatta
    74881 gatggagaag aaccgaatat cttctcgact catttctcag gtggtgcatt gatggattta
    74941 ggtgtttacc ttgtatatgc agctgttgct tggtttggaa tgccaaaaga agtccattat
    75001 ttcccaagaa agatttctac tggtgtagat ggtcttggat gggggatctt acgttatgag
    75061 aattttgatg tatcgatcca gccaggtaaa atcggtgatt cttatttacc gtcagagatt
    75121 tattttgact caggaacgct agtgatgaat ggagtcaatg caattgaaca agcacaattt
    75181 catgatcgcg cgcataaaga aatcattgat ttggcggtaa cagctgcaga aaatccaatg
    75241 gcagatgaag cagaggattt tgccaatgtc atggctcatc cgaaagatcc agcttgggga
    75301 ttgttgtatg aagagtggat cgagctagct agaaatgtga accaagtgat ttatgattta
    75361 agaaaaaatg gcggcatcaa attcgatgca gataatgagg aagattttta atgaacgaat
    75421 ttgtacaagt atttcccgaa aattggcaga aaaaatatga ggataaaggg tttatctcac
    75481 cttctcttat tcaacgagca gtttttcagc cactaagtaa taaacaaagt atagtagcgg
    75541 tttctcctac aggttcaggt aaaacactgg cttatctttg gcctctacta ctaaacgtgg
    75601 aaccaggtga agcaagtgcg ttggtcattt ttgcttcttc tcaagaacta gctattcaag
    75661 tagcagatgt tgcacgtgaa tggggaaaag acaaagagtt gaaagtccaa tctttggtgg
    75721 gcggagccaa cgtcaaacgt cagattgagg gtttgaagga aaaaccagaa atcttgattg
    75781 gtacaccagg aagaatattg gaattgatga aagcaaaaaa aataaaagca catcaagtga
    75841 aaacaatggt atttgatgaa gcagaccagc tatttgacgc tgggaacagc caaatcattg
    75901 atcaaattct tcatcaagct ccaacagagt atcagctagc ttttttttca gcgacagcag
    75961 atcgttccct tgaaagcatt gaaaaaatca ctgggaaaac gtttgagaca attgacgtga
    76021 cagcagagga cgattctcga aaaggactga gacattactt tttacgtgta ccgatacgaa
    76081 agaaagatga ctacttgaga cgtctcacgc atatagaaga ttttcatggt ctgattttct
    76141 ttaatcaact gactgaatta ggcgtaatgg aagaaaaatt gctgtataga ggtgtaccgg
    76201 taggaagtct ggcctctgat cagaacaagc tactccgaaa agcagcgcta gaacaattca
    76261 aaaagaaaat ggtcagagca cttttgacca cagatgtggc ggctagaggt ttagatatta
    76321 caggtcttcc ttatgtagtg aatgcagaag ttccgttaag tgaagaagct tatttgcatc
    76381 gttcgggaag aactggtcga atgggtaatg aaggaatagt aattacactc gtacaagata
    76441 ataatctaag agatttaaaa cggcttttac gtccatcaaa tataacactt gaagagattt
    76501 ttttatacgg aggtaagctg caaactgagc agccaaccaa agaagatgca gctcctgcag
    76561 taaataaaaa gtatgcgaaa actttttctg gaaaggagca gaacccagaa tataacaaag
    76621 aaaaaataag aaaaaaagtt aaaaataaat caaaaaaaga gagaaataaa ggagcaaggc
    76681 gcaaataagc gcctttttat gctatgatta tgaaaacggg aacaaaattg gttgccagag
    76741 gaggggttat gtgaaagaac gtcaagaaga gatcttgcag ctattgacag aaaaccaaca
    76801 aggattgaca gcaagtgatg tagctgagcg cttaactatc gatcgaagca atgcaagccg
    76861 atatttaagt gaactataca aggcccatca tattgtaaaa accgctggcc gaccagtcgt
    76921 ttactctttg ccaacggaaa agtcaaaatc tgatgaagtg catgtcgact cttctacgca
    76981 agtgactttt gaaacactcg ttggagaaaa tgattcttta aaagtcagca tacagcaggc
    77041 aaaagctgcg attctttatc caccgcgggg gttgcatacg attatttttg gagaaactgg
    77101 tacagggaag tcgatgtttg ctgaatgtat gtatcatttt gcaatcgatt cagagatgct
    77161 ttctgcagat gcgccatttg tttctttcaa ttgtgcggat tatgctcaaa atccgcaact
    77221 gctctttgga cacatctttg ggatcaaaaa aggggcttac acaggagcag ctcaagatag
    77281 tcccggtcta atagccaaag cagacggcgg tatattgttt ttagatgaga tccaccgttt
    77341 accgccggaa gggcaggaaa tgctgtttac tttcattgat aaaggtattt accgtccgct
    77401 tggagaaagt gcacaagtac atgaagcatc tgttcagatc attggggcga cgactgaatc
    77461 ttcagaaagt ttcttatcta cgttcaatcg acggattcct atggcaatca ccttgccgaa
    77521 tcttgcttca cgttcactgg atgagcgtta tgaaatcatc tctcttttta tcaaacaaga
    77581 agcgaatcgt ttgaatcaac gtatcgatat cgaaaaagaa gcgattcttg ccttcatgct
    77641 ttatgatgcg gaaggaaata tcggtcaagt gaaacgagac ttgaaactag tctgtgcaaa
    77701 atctttttta cattaccgta ctcatcatga gaaaaagcta atcattcgaa aagaagaact
    77761 ttcccttcaa gtacaaaaag gattactgaa aatcaaggaa ataccggaac gtctcgaccg
    77821 attcatggac agcaaaagtc agtatttgac atttgaacca ggatttgctg atgttgtctg
    77881 gtcacaagat ccagaacgaa acatgcaggt ctataacgat attgaggaaa aagtcacttc
    77941 tctttccgaa accggtgtgg agaacattga tctagagacc ctcatttcaa aagatgtgga
    78001 tgcttatttc cagacatatg tcaaagaatt gactaaaagt tcgattccca aagatctttt
    78061 gccagatgaa atctggcggt taaccaatca attgtatgat gtggctgaaa aacggttaga
    78121 ccgtatttat aatgaaaaag ctcgttttgc ttttgctttg catctgcaaa gtacattgga
    78181 tcgtgtgaga gaaggtcata tgatcgtcca cccagatttg aataacgtac ggaaaaattt
    78241 taagagggaa ttccaggtag caatcgatct ttcaacgatc attgaagaag aagaacaagt
    78301 tgagattcct tttgatgaaa tcggctttat tagtatgttt ctttctatca atctaggaga
    78361 aagtgagcct gttaaagaaa acaaagtaga tgttctcata ttgatgcacg gcagacagac
    78421 tgcttcaagc atgttggaga tggcacaaga actattagga acaaaagtag ggaaagcttt
    78481 taatatgcca ctggttatgg aagttcaaac aatgtatgaa gaagtgttac aatatgtcaa
    78541 aaactatcaa gagcatttga cgaatggcct actcttgtta acagatatgg gatcattaaa
    78601 caccttcagt gatttgattt atgaagagac tggcattcgg acgaaagcta tcacgatgac
    78661 aagtaccatg attgtgatag aagcgatacg gatggcaagt gttggaagaa gcttagaaga
    78721 tatttatcaa aatatccagc tttcctttga atcgatcgta cgtgatcagt tcaaagcgga
    78781 aaagtctgaa actttacagc gtaaaaaagc cgtaatcgtg acatgcttta ctggtgaagg
    78841 agtagctgcc aaattatatc agcgtattat gccagtgatt aatcaaaata aggtagaact
    78901 gatccagatg cagtttttag aacgtgaatc attcaaaaaa cacatcgatg ccttattgga
    78961 agaatatgag atcaaagcaa ttgcgggaac agttgatgtt gattatcaaa atatcccatt
    79021 tttttcagcc tatgacgttt ttgatgatga aaaactaaat attttaaagc gtatagctga
    79081 tgatgagatc ccgacagcta aaattgtcga ttcacttgct ggatcattaa gcgatgtctt
    79141 ttctattgat gaactgatcc gttcattgca aaagacagtt cagcagatcc agaccgattt
    79201 gtcgattatc gtggaacctg gagtagatac tggaatcgtt atccatttag ccttcatgtt
    79261 agacggattg atcaaacaag aaagccatcg gcaatttgag aaactgtcta ttttctcaaa
    79321 aatttatcgt ttggaaatgg atatcgttcg ggcaaacttg atggcactag aacgaaaata
    79381 tcaggtaaaa attccagaag atgatatcgc atttcttaca caaatgtttc ttgaaaataa
    79441 aataaaaaaa tgaacacact aatcatactg tttacacact aaacatctgt gtgcgcacta
    79501 taaaaaagag ctaaaaagca tgaaaacgct atttcagctc ttttttttat tggcatgctt
    79561 cttgcgttat ataaaagtgt aaaaaaataa aaggagtgtt aaaaatggca aaaaaaacaa
    79621 tcatgttagt ttgttcagca gggatgagta cgagtttatt ggtcacaaaa atgcaaaaag
    79681 cagcagaaga aaaagggatg gaagccgata tttttgcggt ctctgcctca gatgcagata
    79741 ataatttaga atctaagaat gtcgatgtat tattattagg tcctcaagta cgtttcatga
    79801 aagctcaatt tgaacaaaaa cttgcgccta aaggtattcc attagatgtc atcaatatgc
    79861 aagactatgg catgatgaac ggagaaaaag ttcttgcaca agcagaaaat ctaatgaaat
    79921 aaatcaagta tgcagttggg ggtataacaa cgtggaagat caacaaaatc tagaagccat
    79981 catggggtta atcatgtttg gcggtaatgc aaagagcgat gcaatggaag cgattgcggc
    80041 tgctaaaatc ggtgattttg agttagcgga taggaaaatc gctgatgcag aagaatcact
    80101 tatacaagcg catcatgcac aaacagacat gttgacacaa gaagcacaag gaaatcatat
    80161 gcaagtgaca ttattgactg ttcacagtca agatcactta atgacatcta ttgcgtttac
    80221 tgatttagca aaagaaatca tcgaacttta tcgtcgcgta tacagcgaat aaatacaact
    80281 tcttttttga acggaagaag aaaaattttt gaacggattt tttattttgt tataaggaga
    80341 actctcttta tataaaaaac ttataggagg aagtaaaaaa tgaacggact atcaagttgg
    80401 atggagaaat acatcctgcc ggttgccgca aaaatcggag cgcaaaaaca tttagttgct
    80461 ttgcgtgatg cttttatcgg cacactgccc gcaacaatgg cgggatcagt cgctgtaatg
    80521 atcaatgcaa ttattcgtga tttaccgcaa cagtttaaca gtacctatgc tgacagttta
    80581 gcagcagaca aaggaatctt tgccgtattg aatgtcatca tcggaatcaa tggttttgtc
    80641 tggaacggta cactagcaat cgccggattg atctttgcct tttcttgggg gtataatctt
    80701 gcccgagctt atggagtaaa tgatctagct ggggggatcg ttggtttagc taccttgatt
    80761 caaggtattt cctttggtcc agcattatca gcagcattag atgtgaatgt tccttcaaat
    80821 gtcgcagatg caatcaatag ttctgagatt ggtgcaacga ttgcagatgg cacactgtca
    80881 gttggattat ggggttggct aaaactagat catttgaatg gaaacgctta ttttacagtt
    80941 atgatcatgg gagctatttc agtcattatt ttttgtaaat tgatgcttgc caacattacg
    81001 atcaaaatgc ctgattcagt accaccagca gtatctaaag catttgcagc cattttacca
    81061 gcgacgatcg ctttatacgt cattgctatt atcaattata tcgtaggtcg tgtagctgat
    81121 gggcaactga tcattgattt ggtgcaaaaa tatattgcag aaccattcct aggattatct
    81181 caagggttgg gagcagtatt gatcgtcaca gtatttgttc aagtattctg gttctttggt
    81241 attcatggac caaatgtatt ggcaccagtg ctagagggaa tctggggaca agcacaacta
    81301 atcaatattg atattttcca aaaaggttat aacggaaaaa ctggtacacc agccgtgtta
    81361 gatgcaatcg aagatggaaa agcttatatg tgggtccgcg gatcgttcga tgcttttgca
    81421 tggtttggcg gatcaggcgg aacgatcgtc ttactgatag ctatcttgat tttctctaaa
    81481 cgtgcagatt atctaacagt agctaaatta tccattgggc ctggtatctt caatatcaac
    81541 gaaccagtga tgtttggtct gccaatcgta ttgaatgcaa tcatgtttat cccattcttg
    81601 attgctccag tagtagcaac agcaattgga tggacagcta cttatcttgg cctagtcgct
    81661 ccggtatcgc agcaagtcac atgggtagta ccgccgttct tattgtcatt cttagcaaca
    81721 ggagcggatt ggcgggcacc gatcgtaaca ttagtatgta tggtcgtgac tttcttgatt
    81781 tgggcaccat ttgtcatctc tgcaaacaaa atggaccctg ccgcaggcga acaacaataa
    81841 gatgaacgtg aggagcggga cgctttaagt ttcgttcctc ataatgaatt ttcgaggagg
    81901 aactgaaatg attacaattg ccagtgcaat gcaacaagat gaaatcaaag aattgttaga
    81961 aggatacaat gaagaaggaa tgacttttac atttaaagaa aaacaaggaa tcaagctgtt
    82021 ctttgaaaca agtgcagaag atccggaagt tgcggctaaa aaagcaaagg aactaatcaa
    82081 agcacagcct tggggaacag tcctttattt tcaatcaaca gctgtttaga aaatggttta
    82141 aaaagatagg atggttgaga tgataaaaaa cggtttattt ttatgtagta ttggttcttt
    82201 gatcgtgttg tatagtttga atccacattc gcaaaggtat gatttgtata gtatggtcac
    82261 aggtcttttt ctaacaggat taggaatcta tttcttcatg aaggggaaaa aaagagaaga
    82321 agcaaaggag aaaaataact aatggaaatc acgaaaaaat taaatattcc agcatcgttt
    82381 ttttacgata aagttatgga ctctgtcctt ttcgatgtac gtaaagcgac cggcaaatca
    82441 gtaactagaa aacagctgaa aaatttggag tacgtcaaac aattctcgca aaataaccgg
    82501 gctcgcataa aaatcgaaga agtcattgaa aaccagtctt ataagtttcg tacatcgaca
    82561 acgaagaatg aattcgtcgt tcattatcaa atcaaaccat tggacgattc gacctgtgag
    82621 gttcattaca cagaaaaaat ggagtcttat ggcatgcttc aaaagttgaa tgatatgctt
    82681 ttaggaacaa tcttgatgta ttttaaacgg agacgtttca aaaaaatgct agatatgatc
    82741 gaagcaacgt attaaatcaa tgaaacatgg tactatttag ttattgaaaa atgaagtaag
    82801 ctagaaggaa tttttcatag gaagatagat ctttcctgcg aatcctcact agcttttttg
    82861 taggaagggg cagcaaaatg acaaacaaag taagagagct aggtgtatct tcgattgagg
    82921 aaatgtttga tttagccact tatgcattta atggggcaga tacacctgaa aggcgagaac
    82981 gttttcgtac acttgcggag aattcttgga attacggatt ttttgatgag gaggatcggc
    83041 taacaagtca agtcatggcg accccttttc cagtgaattt ttacggacaa gagtacctaa
    83101 tggccggcat tggctatgtt gcttcttatc cagaagcaag gggacaaggc gggatcaatc
    83161 agatcatgga aaagatcctt gaagattgcc gtgatcgtaa agtaagctta tcttatctgg
    83221 cgccattctc ctatcccttc tatcggcgct atggctatga acaatcattt gataaaatca
    83281 gttatcaact aaacagtcgt gatgtgccgt ttatcaaaaa aaaatctgga aaaataaagc
    83341 gtatggattt tgaagatgca aaagctgcaa tcagaagaat ttatgaacag atgccggaaa
    83401 accaacgagc tggtctgaag cgcgaagact ggtggtatac gtataaattc aagcaaaaag
    83461 gcaattacca gtttgctttg cattttgatg ataatgagga agcaacggga tatatcgttt
    83521 atcagcttgc aacgtcttcc tttattattg cggagtgggg atttttggat catgatgctt
    83581 tttgcagcct tgttcgcttc gtaagttctc acaatggtgc ttttgaaaca tttgaatatt
    83641 catgcggcta cggcggtaat gatcaaaatt atctattaga aaatccattt gcttctgtaa
    83701 tgatcacacc ttatatgatg gcgaggatcg tagatatttc tttgtttttg gaaaattatc
    83761 catttaaaaa gagacaagct gcatttgccc tacaaataga agaagatcat tatgcgaaat
    83821 ggaatgaagg aatcgtggaa gtaacaatcg aaaatggaaa aaatcatctc tcaaaagtag
    83881 aacaaacaaa actcccaatc gttcgaggaa gtatccaaca tatcacgcag ctattattag
    83941 ggtatcgtaa agtggaagag ttggtattcc aagaaagaat acaggtgaca aaagaatatg
    84001 aagaaacgtt aagctcaatc ttgccacagc aacagccaat tcttaatgac tatttttgat
    84061 aaaaaacggg tgatgacaat atttttgtca ccaccctttt gtttttcatt tggcatttga
    84121 aggaaaatgt taaagtaaat cctctttttt cgtaaatttg aattttttgt tttcttttgt
    84181 tttagggttt tcttttttgt ggtcttggat tgcttctttc gtaatctttt cccgattttt
    84241 tttagatgaa tctaagcgtt ctgcgtatac tttttctttt ggcttatcat tcattggaaa
    84301 ggacctccct acattatttc attctacctc tagtttatca tctgttaagg actagtggaa
    84361 acgttttgcc taacttataa gctgattttt ttagcagata agcttgcaag taaaaaacaa
    84421 acaagctaca attgatttat tgaaattgtt cctgtagctc agtaggatag agcaatcgcc
    84481 tcctaagcga taggtcgctg gttcaattcc agtcaggaac gtacaaataa cccctgtatc
    84541 ctttgcggta cagggggtta ttttgtataa gtagacattg tagtttctaa actgctatgt
    84601 cctaaacgtt tcgatacgct aagtatattt actccttgat aaagtaaaat agatgcatgc
    84661 gtatgtctta gtccatcaat aaacagtcga agagaaaata gggaaaaatt gattactaaa
    84721 gaaaaactta ctaggtcgac ggtgtagttg attttcatac ttcaatagat tcgaattaaa
    84781 cgatttcgat tggcttatac cagtcctatc caacgaaaga tatatcgttc ttaactctag
    84841 cggtataact tgaagcttta cgtaaagcca atgggaatga actggagatg actttttatt
    84901 atttttagta atagctgatg ggttaatact tacaactttc cttttcaaag taccatataa
    84961 aaaaacggtg gggttgttta gcgaaataaa ggatctaagc caatagctta gatcctttat
    85021 tctatgcttt atattatttt ttgcatgtgt gcgaaagtat actcaagccc aatcgccatt
    85081 tcggaaaatc ggtactgtag agccatcttc acggattccg tcgatatcca tcttgtcaga
    85141 tcctaccatg aaatctacgt gggtttgact tctattcaat ccagcttctg ctaattcttc
    85201 atcggacatt tctgtaccgc ctttcacact gaaggcataa gcagaaccta gtgctaagtg
    85261 attcgatgcg ttttcatcaa ataatgtatt gaaaaagatg atgccagatt gagaaatagg
    85321 tgaagggtca ggtacaagtg cgacttctcc taaacgacgc gcaccttcat ctgtatcaag
    85381 cagttttgct aagacatctt ctccttgttc ggcagaaaaa tcaacaactt ttccatcttt
    85441 aaaagtaaat ttcatacccg agatgatggt gccagcataa ctcagtggct ttgtacttga
    85501 aatgtaacca tctactcgat gactgtcagg tgctgtgaac acttcttccg tcggcatatt
    85561 tgccatgaat ttttctccac gagcattata gcttccagcg ccttcccaaa gatggttttt
    85621 aggcaatccg ataatgatat ctgtaccggg agcagtatag tgaagggcag aaaattgttc
    85681 tcgattcaat tcttctgctt tttttgctaa tttttcatca tgcttcttcc atgctaagac
    85741 agggtcttct tcatagacac gagtcgtttt gaaaatctga tcccataatg cctcaacttg
    85801 tttttcttct ggcagttctg ggaagacttt tgctgcccat tgtttaccag ccgcagccac
    85861 gacagtccaa ctgactttat ttgcttgagt tgccttgcgt aaattcatta gtgctttgcc
    85921 attcgctgat tgataagacg ctactcgatg gctatctaca ccagcaaatg catcgggatc
    85981 ggcagaaaca acgctgatcc tgcttgctcc tttttcaagc cattcatccg cttgatcgat
    86041 tttagattgt ggtacattct caatacgatc cgttgcagca tgtaaaagaa attctctttg
    86101 gatctggtcg tcagtccatt gaacgataac ctcagcggct cctagtttat aagcttcttg
    86161 tgtgatcaat cgagcaaggg gtgcctgttc cacgctgatc tgtaaaacaa ctgtgtgtcc
    86221 tttttctgtg gctacgccag tttctgcaat taagcgtgca tattttttta gaagttcatt
    86281 aaaatcagat aacataataa aaattcctcc tcaccatttt ttctggtaac tattagcatg
    86341 ataccattta attatgattc aaacaatgag gctttaaata aaaaaaggag gttatttttt
    86401 taaggttaag tttcatgata tatgttacta cttgctattt cagcttgtga agaaaatcaa
    86461 aaactaattt tgcatatcaa aaaaacatgt tacaatgaaa ttaatcaatg gtggaagcgt
    86521 tataaaaaaa ttaaggagat taaaaaatat ggcaagaaaa aagacgatta ctagagaaca
    86581 gattttggca gctgcttatg aagtagctgc caccgatgga tttacacgat ttactgcgag
    86641 aaacatcgcg aacaaaatga attgttccac gcaaccgatt tatcttgagt ttaaaaatat
    86701 ggatgacttg agacaagctc tttttgagca gatctatgat catttacaat atgaagtgtt
    86761 cactgttgag catacaggga atacgattat cgatcttgct tttaattata tccattttgc
    86821 aagaagagag aagaagttgt atcgttcact gtacttagaa gaatatggtg gaggaaaaga
    86881 gatgcagaat ttctcttata cttactttgt gaatacagtg aaacgcgatc cagcttatgt
    86941 gaatctttca gaagagcaga tcgaatcttt gtataatggg acatggatca tagctactgg
    87001 gatcgcagct ttgatgtctt caagcatcat ccatccaact gatgaacaga ttgaaaagat
    87061 gatccaagac agtatagatg ctatcttgaa attcaaagaa ccaatcgaaa tcgattgaat
    87121 atactcaaaa gttccacaaa tcagtaggta acaagagatg tgtcaaaata aggaaaagtt
    87181 cgtttttctt acagcgtatg catttttaac tcccgaagaa ctcttccatg aatgcaatga
    87241 ataaaagagc catacagatt gaataaatct ggatggctct tttattgcaa gtatattaaa
    87301 tttttgcgac tagttcttta gcgaaagctt ctaaattttc gatatcttct tcttcagcat
    87361 tcagatccac tttaacattt tcggctcctt ttgatgcgcc tgtttttgca aaagcagcat
    87421 cgaaatcatc caccgatttg cagaattcat cgtaaaaagt atcaccagat ccgcagacac
    87481 cgtaaatttt tcccaataga tccaattctt gcaagtcttc ataaaagtct acgatctcat
    87541 caggcagatc gccatcatcg tatgtataag tagctactac gcaaatatct gcctcctcga
    87601 attcatctgc atctacttgg gtacattcgt tgatttcgac ttcgatatcc agattttcaa
    87661 atgcttctgc aacaatatcg gcgatttctt cagtgtttcc tgtcatgcta gcatatacga
    87721 tttttgctaa agccatgatg tgcctcctcg tttataatta tttaacaatt atgaattata
    87781 ccaaaacatc attgaaaaga aaataaaacc taaagacacc tggggaatgt catttggcta
    87841 aatgtatgcc gctatgttaa aatgagaggg atttgtaaaa aggagacgaa aatatgatta
    87901 ctttaaaatc agcacgtgaa attgaaatga tggcagaatc tggagcttta ttagcagatg
    87961 tacataaaaa tctgcgtgat tttataaaac caggaatcac aagttgggat attgaggtat
    88021 ttgtcagaaa ctatatcgag agtcatggcg gaattgctgc gcaaattggc tttgaagggt
    88081 atgaatatgc gacttgtatc agtataaatg atgaaatttg tcatggattc cctcgtaaaa
    88141 agcctttgaa aaacggtgat ttggtaaaag tcgatatgtg tatcgatttg aaaggcggca
    88201 tttctgattc ttgttgggcc tatgtagttg gagaatctac acctgagatc gaccgattga
    88261 tggaagtaac gaagaaagct ttgtacatcg gtattgagca agcacaagta gggaatcgga
    88321 ttggtgacat cggtcatgct atccaaactt atgtagaagg cgaaaatcta gcgatcgttc
    88381 gtgattttat tggtcatggc gttggtccta cgattcatga agaaccagca gttcctcatt
    88441 acggagaagc tggaaaaggg ttgcggttga aagaaggcat ggttatcacg atcgaaccta
    88501 tggttaatac cggaacttgg aaaatgaaaa tggatcctaa cggctggact gcgtatacaa
    88561 gagacggcgg actttcttgc caatatgaac atacattggc tattactaaa gatggtccaa
    88621 gaattttgac atcacaagga gaagaaggca cttattgatc tatccagtca ataggtacgg
    88681 gagaggtaca tgaaagtttg gaacaaaata aaacaaaatg aaaatctgat gcgctttatt
    88741 gagacgactc aaaagcgcat cactgattca gaaatgagta cgacctctgt cgtcgttgct
    88801 tattatttac tcttgtcttt atttccattg attattgctt ttggtaatat tttaccgttt
    88861 ctccacatcg atcaagagac tgtactgacc tatttacgag aagtgattcc tgagaccgtt
    88921 taccagttta tcggtcctgc aatcaaagac cttttgacac aaagttcagg aggcttgtta
    88981 tccgtttctg cattagcggc tttatggtct gcaagtcaaa gtatcaatgc tttgcaaatt
    89041 gcgatgaata aagcgtatgg cgtagaaaat agaaaaaatt ttttgatcgt ccgtttcttc
    89101 tcactaattg tcattgtctt gttcatgatt gctattagtg gggtaactat cgttcttggg
    89161 ttgggacagc tgattttgga tgctattcag ccaatcgtac aaatacccgt gaactttatc
    89221 gatcagttcc aaactttgaa gtggccaatc acattggtag ctttatttgt catcatgttc
    89281 ttgatttatt taattgtccc taatgctcag ttgaagttga agacgatcat tccgggcacg
    89341 atcttcgcta ctgtcggctg gatgctgtta tcacaagtgt ttagtatcta tgctaggtat
    89401 tttgcaacga ggatcagcgg ttatcagatc ataggaagtt ttattgtact catgctttgg
    89461 cttaattttg ctgctacgat catcattttg ggcggaatca tcaatgcagt tgttcaagaa
    89521 tacgtgacgg gtcatcaaat caaaaaacgt cgagatccaa gccgaaaatg gttcaataaa
    89581 ttgaaaacta aattttctca taaaaaaaag tagaaacaag agttcttatt gtaaatttat
    89641 atcagtaatt tataataatt atgactctta attaaagtag gtgcccatat gcttgtttct
    89701 tttagtatgg ttataagatt ttttctgaca attttattat cactgatatt aacacctgta
    89761 ttcaaaatca tttcagtaca aacaggtatg gtcgacaaac caaatgaaag aagaataaat
    89821 aaagttccaa tgccttcagc gggcggactg cctatcttta tttcatttgt tatatcgact
    89881 ttattcttat tccgtaaaat tatccctcaa tcgtatattg taccgatctt gattgcttct
    89941 ttagtaatca tagtcacagg agtgctcgat gataaatatg agctgacacc taaacaaaaa
    90001 agtataggga tcttgattgc ttctcttatt atttattttg tagcagatat acgtattgat
    90061 tccttcacgc ttccttttat tggatatatc caactaggct ggcttagttt tcccgtaacg
    90121 attttttgga tcttcggtat cacgaatgcg gtcaatctga ttgacggttt ggatgggctg
    90181 gcggcaggga tttcattgat cggtttaatt acgataggaa tcattggata ttttttcctc
    90241 catgcttcta cagtgtatat tccagttgtt attttctgtc ttgtagcaag tattatcggt
    90301 ttttttcctt acaattttta tccggcaaag atttatttag gcgatacagg agctctattc
    90361 ctagggttta tgatggccgt cctttcacta caagggctga agaacgtgac ttttgtttca
    90421 tcgatttctt tgctgatcgt catgggagtg ccggtaacag acacgttttt tgctatcatt
    90481 aggagaaaag caaaccgtgt ttctttttct acagcagata aaaaacatct tcatcaccga
    90541 ctaatcagtt taggctttac tcataaaggt gctgtcctga cgatttatgc gatggctttg
    90601 atgttttctt tcacagctat ggtcatgaat tatactggaa gaatcggaac gatcatattg
    90661 attattgcca tgttgtttgc tgcaatttta ttatttgaat tgattggtct gatcaatgag
    90721 aaacatcagt ttattttaaa aacattgcga tttttaggga ataaagagta ccgtacgcaa
    90781 gtaaaacaga aatatttgaa gaaatggaac cgtcatatag atgaataaca aattaaacca
    90841 gccgatcctt attagtattg tgacggcaaa cagtgataaa atttttcgga cactagataa
    90901 ttttatttca actgtaaaag atgaaacagc agtaaaaatc cgaatttttg acaacaattc
    90961 acttcccgag tacaaaaaaa gactaacaga gtataggaaa tatccctaca tcgatatcta
    91021 ttttcatgat gaaaattccg gatttggtta tggacataat cacaatctga tagctagcga
    91081 ttctcgctat ggcattattt gtaacccaga tattttagtg gatgaagaga cagttacagc
    91141 attggtcttg ttattgaaag aacatccgga atgtgcgatg ttagcaccta agattcttaa
    91201 tgaagatgga acaactcagc acctcatccg tgagcagctg gctgtttttg attacgtttt
    91261 gcgctttgtg cctttcaaat gggtcaaaaa aaagtttgac aaacgactgg ctgtgtttga
    91321 atgccgagga ttgcctactg atgctaatag ttatgtacgg atgatctcag gtagttttat
    91381 ggtagctgat attgataaat ttaaagagat aaaggggttt gatgagcgtt tcttcatgta
    91441 ctttgaagac aacgacttgt gtttgagttt tgaaaaagca ggttacaagt tattgtacac
    91501 acccttttat gcagttatcc atttttacgg gaagggtgcg catcgaaatg caaaactgtt
    91561 tcgcgtgttt ttgacttcta tgatgaaatt tttcaataag tgggggtggc gttttttctg
    91621 atgaaaccag aaatattagt agtcgttgtg ctttatcagc aactattcag tcaatctcca
    91681 gcttatggac cattaagcaa agcacttagc gaaaagcaaa ttcagcttat tatttacgat
    91741 aatagtcctc aaaggcagca aaatgagctg tttgaaaaag aaaatatcgt ttatcatcat
    91801 gatccgcaaa atcctggttt agcggtggct tataattttg cgttgtctca agcagatgaa
    91861 ggaacacgtt gtctcgtcac tctggatcag gatacgaggc tagtcgacga ttattttgaa
    91921 ctattgcgta aagtaacctt tacagatgaa tgtgttgctg cagttccgat gattttttct
    91981 gggagccgac aagtttctcc cctatattca gatcgttata tcaatcgtac agctcaagca
    92041 gtagaagttg aaggcccaac agctgaacga atcatggcaa tcaattcagg tgttgcctgg
    92101 tctgtcaaat ttctaaaaaa gattggcggg tttaatcctg ctttttcttt agattttttg
    92161 gatcattggc tgttttggaa agtggatcag ttggaaaaaa ctgtggagat ccttcctgta
    92221 cgaatggaac atgatctttc ggttttagac tatgaaaaag tttcaagtaa tcgataccgt
    92281 tctattttac aagcagaaaa tcgattttat catgaatatg atcaaaggca tttatccagt
    92341 caccgacgac aattattgtt aagaacagct aaacagttct tgaccgtcaa aaaccggcat
    92401 atctggcgga tgactttgcg ctcatatgtt aagaattgga aggtgtgaat tcgtgcgttc
    92461 ggtttgtata gctaccttta atggaggcag atatttaaga gagcagttag atagtatttt
    92521 atctcagctt tcagcagaag atgaagtagt catttctgat gatgggtcag tagatgatac
    92581 atgggatata ttaactgaat acgcagaaaa agataagcga atccagcttt tttcggggcc
    92641 tcgtcaagga ttgatcgcta attttggtta tgcgatccag cagacgaaag gagatctaat
    92701 ctttttggca gaccaagatg atgtttggtt ggagacaaaa gtgaaaaaga tcactgacta
    92761 ttttgagcag aatccggatc agctagtagt agtttctgat ttaatcattg tggacagcga
    92821 gctgcaagag atccatgatt cgtatttttc gtttcgaaat tccaaagaag gatggcttaa
    92881 taatgtgata agaagtcact atattggtgc tggtatggct ttccggtctt ctttaaaacc
    92941 cgatattttg ccgatcccgt cagaagtgcc gatgcatgat atgtggattg gtttgctggg
    93001 aggaaagaag actgggttcc taagagaacc gttaactctg taccgaagac atgatttaaa
    93061 cgcgagtgag attgaaacac attctaaatt acggcaaaaa atttcttggc gtgttcatct
    93121 gattacggcg ttgatcaaac gaaaatggtt taaacgttga taaaagatgt ttaaatcctt
    93181 aatatttcca tgacaaatat aacaattgat gagataataa caaagtgaaa tttgaaatca
    93241 aaaaggagag cagatatcca tgaagggaat cattcttgct gggggcagcg gaacacgtct
    93301 gtacccttta acaaaggcaa catcgaaaca attgatgccg atttatgaca aaccaatgat
    93361 ttattatcca atgtctactc tgatgttggc gggaatcaaa gaaattctga ttatttcaac
    93421 gccacaagat acaccacgtt tcaaggaatt atttggaaac ggaaatgatt taggactgca
    93481 gatcgaatat gcagtacaag aaagtccaga tggattggca caagcgttta tcattggaga
    93541 agaatttatt ggagatgata gtgtctgctt ggtattaggg gacaatattt actatggtgg
    93601 cggtctgtct aaaatgttgc aacgagcagc ttcaaaagat acaggcgcga cagtatttgg
    93661 ctatcatgtc aatgatccag aacgctttgg tgtagtagaa tttgatgaga atatgcaagc
    93721 tttgtcgatt gaagaaaagc ctgctcagcc aaaatcaaat tacgctgtaa ctggccttta
    93781 tttttatgac aatgaggtaa tttctatcgc taaaggtatc aaaccttcag aacgtggaga
    93841 actagagatt acggatgtca ataaagcata tttggataaa ggaaaactat ccgtagaact
    93901 tatgggacgc ggatttgctt ggcttgatac aggaacacat gaatcattgc ttgaagcatc
    93961 tacctttatc gaaacaattg aaaaacgtca gaacttaaaa gtggcttgtt tggaagaaat
    94021 tgcttaccgt atgggctaca tcgataagga acaattgctg gcccttgcac agccattgaa
    94081 aaaaaatcaa tacggtcaat atttattgaa tttggccgct gaataaggag tccttacaga
    94141 tgaaagtaac agatacaaaa ttaacagatg tcaaaatcgt agaaatggac gtctttggcg
    94201 atcatcgcgg tttcttcaca gaaagttatt caaaagcaaa atttgcagaa catggattga
    94261 aatatgactt tgttcaagac aaccattcac tatcagctga agctggtgtc cttcgggggc
    94321 ttcatttcca aaaaggagaa gctgcacaga caaaattgat tcgggtagtc actggagcag
    94381 ttttagatgt agtagtggat atccgcaaag gtagcccgac ttatggtcaa tgggacggat
    94441 ttatcttatc tgaacataat catcgacagt tgcttgtacc taaaggatat gcccatggat
    94501 tcgttacttt gacaccaaat gtgaacttcc tttataagtg tgataattac tataatgcag
    94561 aagcagatgg gggcatcgca tttgatgatc cagatttagc gattgattgg ccaattgatc
    94621 catcaagagc gattatgtcg gaaaaagatc accgtcatcc gacattaaaa gaatttgaag
    94681 cagaaaatcc atttgtttac ggagaaatct aagaattaag gagaaatttt cttatgaaaa
    94741 acatcattgt taccggaggt gccggattca tcggctctaa tttcgttcat tacgtggtaa
    94801 acaatcatcc agaagtgcat gtcactgttt tggataaact gacttatgca ggaaacaaag
    94861 agaacttggc tggacttcct agcgatcgcg tagaattggt cgtaggagat gttgcagatg
    94921 cagaattagt ggaccgtcta gtaaaagaag cggacgcagt tgttcattac gcggcagaat
    94981 cccataacga taattcatta aaagatcctt tcccatttgt acaaacgaat atcattggta
    95041 cgtatacact tttagaagct tgccgcaagt atgatgtacg ctatcatcac gtatctactg
    95101 acgaggttta cggcgatctt ccattacgtg aagacctgcc aggacatggg gaaggcgaag
    95161 gagaaaaatt cactgcggaa acaccatata atccttcaag tccatattct tctacaaaag
    95221 ctggttcaga tctgttagta aaagcatggg tgcgttcttt tggcttacgt gcaaccattt
    95281 caaactgttc aaacaactat ggtccttatc agcatattga aaaatttatt cctcgccaag
    95341 taacgaatat cttaagcggt attcgtccaa aattatatgg cgaaggaaag aatgttcgtg
    95401 actggatcca tacgaatgat cattcttcag cagtgtggct gattttgacg aaaggcagaa
    95461 ttggcgaaac ctatctgatc ggtgcagacg gtgaagaaga taataagacc gtgatggaat
    95521 tgatcttaga aatgatgggt caaccaaaag atgcgtacga ccatgtaaac gaccgtgcag
    95581 gacacgactt gcgttatgca atcgattcga caaaattacg ggaagagtta ggatggaagc
    95641 ctgagtttac gaacttccgt gatgggttgg ctgacacgat caaatggtac gaagaacatg
    95701 aagactggtg gaaaaaagaa aaagaggccg ttgaagcagc atatgccaaa aatggacaat
    95761 aatacagaga aaatgtcact gtattaatgg aaaaacacat gtgaggcagg cgctcgcctg
    95821 tctctttttt aaaataaaaa tcaaggagcg gataatcata tgtttttatt aactggagga
    95881 aatgggcaac taggtacaga attgcgccat ttattagatg aaaaaaatct gcaatatgtt
    95941 tcaacagatg caaaagactt ggatatcaca gatgccgaga aaacaatggc ttacatcaca
    96001 gaattaaaac ctgatgttat tttccattgt gcagcttaca cagcagtaga taaagcagaa
    96061 gaagaagcaa aagaattaga tgagaagatc aatgttgatg gtacaagaaa tgtagcactt
    96121 gcagcaaaag ctgtgaatgc tcaattcgtc tatatcagta cagactatgt tttcgatgga
    96181 aaaaacaaaa atgtagaata cgaagtagac gaccaaacga atccactaaa tgaatatggt
    96241 cgtacgaaat tattaggcga aaaagcagta gaagagattt tagaagacta ttatatcata
    96301 cgaacttctt gggttttcgg gatctatgga cacaatttcg tcttcacgat gcaacgccta
    96361 gcggaaaccc gcgacaaact gactgtagta aatgaccagt ttgggcgacc aacttggaca
    96421 cgtactttgg cagaatttat ggtatatgtc atcgaagaaa aagcaccatt tggtatttat
    96481 catttgtcga acgaaaatag ctgttcttgg tatgaattcg caaaagaaat attgaaagat
    96541 acagatgtag aagttgcacc tgttacgtca gaagaatttc cgcaaaaagc aacacgtccg
    96601 cagtattcag tcatgagcct gaaaaagaca gaagagttag gatttgtcat accaacttgg
    96661 caagaagcac ttgcgcaaat gctggaagct gccaaaaaaa tgaataagta atttcctttt
    96721 atcaaataga atttggagca tgatatggaa aaacaaacaa gaagtgcacg acgaaaagaa
    96781 aagccaaaag cgaaccaggc agattcgaat ttggtgcttg acctgtttct aaaactccgc
    96841 tatcttattg gcgttttagt catcgtggtc atcgttactt ttaatttaaa cggaagttca
    96901 cttggtgttt gggataaata tgtgtcgcaa agagacgatg ggaaaaaaac tgatgtgatt
    96961 tttggacaga atcgggaagt gcggtcagac gaatggctgg tgcagacacc tttttacctc
    97021 tcacaagctg aagaggactt tccgttggtg aacgaggatt attcattaaa cggacaaaac
    97081 atgatcattg cgtataattc tcctgtaaag gatattacgg ttatcggaaa accattcaac
    97141 tggggattct ttttcctcgg aagagatcga ggactttcct tttattgggc gatgaaatta
    97201 gtcgtcatgg tcttgcttgg attcgaagta ctgatgatat taacgaagag aaaaaaagct
    97261 ctatcgctaa tcggtgcatt tgcgctgaca ttttctccag cagtccagtg gtggttcatg
    97321 caacatgtgg gcgatttgat tttctttact ttgggactga tgactgcttt ttatcattat
    97381 ttctacaatc atgataaaaa atggctacga acgttgatga tgcttttggt ggttattttc
    97441 ggtattgggt ttattttagt gatttaccca gcccatcaag tgatgctggc gtacttgctg
    97501 attttttatt ttgttggcct gcttatttac tatagcaaga aagtttcatg ggatctgttg
    97561 gatgtagtgt tgattggagg ggcagtttta tttattggcg gagtcatggt tcatttctgg
    97621 ctaacgtcaa aagacgcatt gttagcttct ttgaatacct tatatccagg aaaacgggta
    97681 agtactggag gaaagtggac gattggcgaa tttttttctt tccttacaaa ttggaaaatt
    97741 ccttttgaag atatcaacta ttcgaataac tctgaagtgg ccctgttcta tcactttttt
    97801 ccaactgtat tcctagcaag tccatttgtg ttatttggca aaaaagatag cgaacaaaag
    97861 ttactgggac gtattttgat gattttttgt ctgttttcta tcttttggat taccgttggg
    97921 ttgccaaaat ggctagcaga aatcactctg ctgtcttatg ttcctccatt aagagccatc
    97981 cttactttta gttttgcggc ttgtttatta tcgatctggt ttatcgctta tatttggaga
    98041 gaagatgtta ttcctatttg gtataaattc attttgttgg cagtaaatgc atggtcttat
    98101 ttctatgcga ttacgcattc gaaaatggat aattattttt cggattttga aaccatcgca
    98161 gttgctgtgt tgggaacagt gattttagct tttgcattat ttggctggag acgcttgttt
    98221 tacctcgctt ttgccgcctt agttatcgta tctggctttt tcgtcaatcc agtcgtagaa
    98281 ggtacaggag cgatttttga aaaaacactg gcaaaagaaa tccaaaaaat cgatcaacaa
    98341 gatcctggta aagtttggtt gtctgaagat gaattatata acttcacccc aacattggga
    98401 gtgaaatcct tcaatacagt acgcttttat ccagatatag ctgcatggaa gaaaatcgat
    98461 ccgaaagaag aatacgagaa gtattataat cggtatgctc atacaagagc ttttatcgct
    98521 aaggaagata caaagttcat tttagatcgt ccagatatgt tttcggtcac gttgaatttt
    98581 gaagacgcag aaaaattagg gatcgactat gtgatcagta aacgaccatt aactgattac
    98641 aaccaattat atgaagcgca gttcacgcag ctatatggac cggataaaga cggttatatg
    98701 atttttaaag tagaatatcc ggattatcca gatgtaccac aaacacaaac tcaggaaaat
    98761 cagctagtcg tacagtaata ggctagctga gaataatcct gaaatgttag tagaaaggag
    98821 agtggagata attcaagcaa tcgtacaaaa atttgtcaac aaaatgagtg tcggtttctt
    98881 gcttttgtta ctgacgctag tcttttttgt ttggacagca gggttaggtt ttccagcaac
    98941 tttgtttata tataacaata gttcattact tatcgctttt agtgtggcta ttttgctcat
    99001 tttaaatgtt tataaagcaa aaggcgtcga ttttctttat ctaggtgttg cgcttgccct
    99061 tttcattttc ttttcttact ttcgggaatt aagaaatgag tctttatttg gagacttact
    99121 gtttcctttg attattttat tttgtttagt aattaaatgg atcgagtgga caaaatggga
    99181 tcgcggtatt tatctgattg tcttttcggt cttcctcgga atcacgttat atcgtgtatt
    99241 tacagagatc caagtaccag aaggacaaag catctgggct ccaggcaatt cgctttctga
    99301 tatttggatc aatacgaata taatcggaag ttctttgatg actcttgctt tattgatcag
    99361 tggatttgcc agttcttttg aacgatggta tgtacgaatc atcggtgcag tagcagttgt
    99421 tgcagctttt ctcgggattt gggtctgcca atctagaggt gcattagtcg cgttgattgt
    99481 ttttgcgata ttggatcttt tacctaaatc gttgatgcgt gtcattcgtg cgccatttat
    99541 tgcttacacg gttacgattc ttttagcttt gccgatttct tatctggcgg ctgtctctga
    99601 aaaagtcaat ttgtttaccg gtcgagaaga tatttggcat aaattctatc agacgctagg
    99661 agaaaaatct gaacaaattt tactaggaat gaaaacattt atcttccaac gaggaaacca
    99721 atttttagga aaccacaata gttataattc tattttgaat atctatggat tgataggatt
    99781 tgggattgct gctttgctgc ttatcctttt tattggtcgc ttaacgttga aagcagattt
    99841 atccaacggg cagatgactt ttatctgggc cttttttgcg gtgatgatgc agtcatttat
    99901 ggaggataca ttgacttcat acccatgggt tccgattatt tatatcctgt tggctatggc
    99961 cgttcatcga tatgatgaac cagaagttgc ggaggaattc tatgttatgg aagaggcata
   100021 tgacgatatg acttatgatg atacatctta tgaggaacaa ccagatagcg ctcattcttc
   100081 caggatccaa cgctatcatt gataggcaca aaatacgaga gaggctgtga caaccgtcgc
   100141 agcctttttt ggtttattgc aattgtctag catcttctgt aaaatagaaa cgttagagaa
   100201 aatcttgtat catagaaaat taagaaagaa ataagaacgc agctcggtac caaaaatatg
   100261 gtaaaatggc acttgactgt aattcactga aaatcgcttg gaggaacaca aatgtttaaa
   100321 gatttgtatt tatttatgaa ggacatttat cagaacagaa aattattgct acagttttcg
   100381 atcaatgact tcaaatcaag atatgcagga tcgtttttag gaatcttatg ggcgtttatc
   100441 aatccgctat ttactgtatt gatttattgg ctggtatttg gtgttggact gaaagcacaa
   100501 atgatcgatg caaaataccc atttatcgta tatttagtta ccgggatgat tccctggttc
   100561 ttcttctcag atgcatttgc atcaactacg gttgtttttc gggaatatac ttatttagta
   100621 aagaaagtcg tattcaatat tcgtatcttg cccacgacga aaatcctatc caatctgtac
   100681 acccatttat tcttcattct gattggcata gctgtcgcgc ttataaatgg tatatatcct
   100741 tcagcaatgt ctttacagct gatttattat ttattttgca tgtgtgcttt cttgacagga
   100801 ttgacttggc tgacggcatc gatccaacca tttttacctg atatcatgca attaattacg
   100861 atcgtgctgc agttgatcat gtggacatta ccgatattat ggagccctaa tcagtttgat
   100921 tcaggtcttg taagcatatt gaaattaaat ccgctgtact atgttgttca aggttatcgt
   100981 gagtcatttt taagtgaagc ttggttctgg gagcatggaa catatacttt gtatttctgg
   101041 gttttcacgt tagtaatgtt cgtaatcggc tctgcagtgt tccgtagatt gaaaccacat
   101101 ttttcagatg tgctgtaagg agaatagcta tgacagaata tgcaattaga ctaaaagata
   101161 tcacaaaatc ttataatatg tacgcaaaac cttcggatcg atttcgtgaa gccctcaatc
   101221 cttttaagaa atcctatcat gatgtatttt atgcgttgaa aaacgtgaat gtagaaataa
   101281 aaaaaggtga gatgatcggt ttcgtgggag aaaacggatc gggaaaatct acaatgttga
   101341 aaatcattac gggagtactg acaccaacat ccggcacgat ggaaatcgaa ggaaaaatct
   101401 cagctttgct agaactagga tcaggattca atcctgagta ttctggctat gaaaatatct
   101461 atttaaatgg aatggttcta ggattttcaa gagaagaagt cgatgaaatg gtcgatgata
   101521 tcattgaatt tgctgatatt ggcgaccata tttatcaacc tgtaaaaacc tattcaagtg
   101581 ggatgttcgt tcgtctagct tttgctgtag cgatcaatgt gaatcctgat attttgattg
   101641 tggatgaagc attggcagta ggagatcttg aattccaact gaaatgtatg gaaaaattta
   101701 cagaaattaa gaactcggga aaaacgattt tattcgtttc tcatgatatt agttcgattc
   101761 gccgtttttg tgatcgttcc tattggttga aaaatggaga agttgtcgaa tatggtgata
   101821 cgatggaagt aacgacgaat tacgaaaact ttttgaagaa aaaatcagtt aaaacagtag
   101881 atcgaaataa aaaccaagta gaacgcttcg cagctccaga tatcgtagac gtgacgaaag
   101941 ccgaattgtt ggataaaaat aaaaatccag tggatatgct ggaacaagga gaaaaactat
   102001 acgttaaagt aacctatgaa gtaaaagacg atacaatcaa aaatccagtt ttaggaattg
   102061 ctctgcgtac agtcgacaat ttttatgtgt gcggactcaa tactctacta gatggtatca
   102121 agatcccttg gaaaaaaggt gagaatatct tttatctgga gtatgaaaag ttaggtttgt
   102181 tagctggtga atattatttt gacgttgcgg tctttgaaga aaatgcaacg gttcctttgg
   102241 tatacaagac aaaatacatg aacttgtttg tcagcggttc ttatatcgga gaaggcatcg
   102301 tggtcttaga ccataaatgg gaagaaggga ctcatagcaa tgaaatatga ttttgaaatg
   102361 gacctagatg aacaaagcag tgtgggaaaa attgccgcac agatcaaacc aggaagtaaa
   102421 gttttagagt ttggtcccgg aaacggtcga ttaacgaaac atttgatcgg agcaaaacaa
   102481 tgtgaagtga gcattgttga gctagataag gaattattcg attttgtcag cgaatttgcc
   102541 caagatggat tttatggaga tatcgaaagt tttgaatggg caaactatta tgctggacag
   102601 acatttgatt acgtcttgtt tgcggatgtt ttagaacatt tagtagatcc tggaaaaaca
   102661 ttgaagaaag tcagagaatt tttgaatgaa gaaggagaga tcctgattac attccctaac
   102721 ttagcacata attctgtcat gatcgatttg ttcaataatc aattgccttg ggcttcttat
   102781 ggtctcctag atgaaaccca taattctttt tatactcatg atggtttcca aaaagttttt
   102841 gaaaaagccg gattattcat taatattgaa gattacttgt acttggctgt aggagataca
   102901 gaattaaaat caacttatga agagttgccg gaagctgtac gctatgattt caaaatgcga
   102961 ccatttggtg aagtctacca atactttttc tcattgatga aacatccagt agtacaaact
   103021 tccattgcac agccacaaaa ctctaattat gtaaaagtac tagaagtaac ccagcaaacg
   103081 aaacaagatg aaacaatcca acaaatacca ttcaataatt tcacagggga aaatgagaca
   103141 ctaagtttcc ccgtgtcaga agcaacagaa agaatggtgt ttcgcttcgc acagcaacct
   103201 agctttatcg aattcagtgc agaagctgct ggagaaaaac tcatatttat cgattcaaat
   103261 gctgtagtca aaacagtaaa tgattgttat ttgtttgatg ggaaagaaat accagagttc
   103321 gctctaacgg atatttctgg gaaagaagtg actatccatt gtcactatcg ctttatcggg
   103381 gaattgacac caacaatgaa agaattgctg gaaacaattc gtcctatggc tgaagtcaca
   103441 aagcagcttt ctgagaaaaa tgatgaatta gaaagagaaa atcagcatct tcttgaggaa
   103501 aatacaagac tagaccatac gttaaaaaca accacaaatc gctattgcac attgttggaa
   103561 agtgatgaat ggacgatcaa acatagacta ggacgacgta agaaaaaaac atcgaaaaaa
   103621 atacaagaaa aagagctttc tatatgtatt gatgagaaaa tatgggatgc agaaacaaaa
   103681 atattaaaaa tcatcggatg gggaatcgct aattcggatc gccagccatt gtcctataag
   103741 ttatcagctg accaatcgcc atttttcgaa gcaattcctg tttcacgaga tgaagtcaat
   103801 caagcagaac agctcgcaaa aggaacagaa gcgggatttg aattacgaat cctttgcgag
   103861 caagaacgtt cattcttagt ggaagcagta gctcaaaatg gtcaatcatg gatcattgaa
   103921 atgtaaataa tgattaactt ataaagataa ggagtaaacg aatcgtgcaa aaaaaagaaa
   103981 ttgccgtatt tatcgatcaa atcactcgtg aacgggccac tggcgatttg cttattgtgg
   104041 gctgggcaat tgatgaagtg accaaagaaa tccctacgat aaaggtagaa aaagaaaatg
   104101 tgattgctga agcaacacat gtcgtaagat tggatatcaa tcatttatat aatcttgatg
   104161 ttaagaccca aagcggtttt aagatacgtt tatccgggaa aatgagagga aaagcgatct
   104221 tagattttca aacggcaaaa catcaaaatg gcatagctgt gaaactaaat ggtaaatacc
   104281 cttatgatga cggcattgaa tcttcatggg aaagaaaaaa aagattactg aaaaaaggga
   104341 taaattatgc aaggactcat ggcgtaaaaa aaacaatccg ccgggtcaaa ctggaattga
   104401 aaccaggatc gatcgactat gcagagtgga tcagtcgtca cgagaaacct gatctgaaag
   104461 agcaaaggaa agaagcacaa gggtttgcat accgtccatt gatttccata cttatgcctg
   104521 tctataacgt tgaaatcaaa tggctcgaaa aatgcattga ttcggtattg gatcaaacgt
   104581 atgatcactg ggaactatgt atcagtgatg atgcttctac tgatccagct atccgaaagt
   104641 gtctggaatc ctatcaagca aaggatgata gaatcaaagt tgttttccga caagaaaatg
   104701 gacatatcag tctggcaact aattctgcgt tggaaatggc agaaggagaa tttatagcgc
   104761 tcttagataa tgatgatgaa ttaccgccat ttgcattata tgaagtggca aaagtgctaa
   104821 acatacatcc agaactggat ttgatctaca gtgacgaaga taaaatcgat gcagatggca
   104881 atcgctttga tccgcacttc aaagcagact ggtctcccga tacattgatg ggaaataatt
   104941 atatctctca cttaggcgta tatcgaacaa gtattgtgaa aaagcttggc ggtttccgta
   105001 aaggctacga aggctctcaa gattatgatt tggtccttcg tgtgacagaa caaatccctg
   105061 cagatcatat ttaccatatt gaccgagtct tgtatcattg gcggacgatc ccaggatcaa
   105121 ccgcaagtaa tggtgaagcc aaaagttata tctatgattc tggagtgaaa gcattgacag
   105181 atgccctgtc tagaaggaat atcaaaggaa gcgtacgtcc tggcagaatt tctggatttt
   105241 atgagattgc ttatgacgtc ttgcaagagg atttggtcag cgtgatcatt ccaacgaaga
   105301 atggctatga agatctaaaa acttgtgtgg attcgattat cgaaaaaacg tcttatccaa
   105361 attatgaaat cattattgca gataatggaa gtacagatcc taaaatgcag gaattatttg
   105421 cagaatacaa gcatcagctg aaagaccgat ttatcgttga attaattgac attccattca
   105481 attattcacg tatcaacaat ctggcagcag aaaaagcaaa cggcaaatac tttttattct
   105541 tgaataacga tacagaagtg attgaacctg actggatgac agcgatggtt tcttatgcac
   105601 agtttgatcg aatcggctgt gtaggagcaa aactgtttta tccagatgat acaacgcagc
   105661 atgcaggagt attattagga atcggtggtg ttgcaggaca tgcattgaat aactatgatc
   105721 gaacacattg tggttatttc ggccgattgg tcattgacgt caattactta gcagtcactg
   105781 cagcttgtat gatggtcaag gcagctgact tcaatgctgt caatggattt gatgaaacat
   105841 tagaagtagc attcaatgat gttgacttgt gtttgaaagt ttatgaactg ggtcgttaca
   105901 atgtctatgc acatcaagca gaattgtatc atttcgaatc taaatcaaga ggttatgaag
   105961 atacaccaga aaaacaaaga cgttttgccg gggaaataaa aaaaatgcaa gataagtggc
   106021 cttcttatat tgctcatgac ccattttata acgataatct aactaaagaa ggaatcggcg
   106081 atttttcatt gagacctgat tgattaaaag aactgtattt tcatttttat tgatgaaaat
   106141 acagtttttt tagtatatga aaattgtaac ttgtgaccga agagatgata ggaagaacac
   106201 ccattcagta ggaagaatgt gatacgattt ttgtccaact ctctgttagg cagaaatata
   106261 catataaaca tacaatagtc accttttcta gtttctttta atttatttta caatttggtt
   106321 attcactagg tttttaaact ttgctatggt aagatgatag aggcattatt ttatggttag
   106381 gagtgtatat atgaataaaa atggggagtg gaatgcggca cgtaggatat tcattatcat
   106441 catggatgta cttgtattga atgtgtcgat ctatcttagc tttttgctga agttccaagg
   106501 agaagtccca gcaagaaact tagagacctt tcagcattca gcagttttta tttccattat
   106561 ctttattgcg ttgaatattt tgttaggagc ttatgtattc tatagccgag tgatcagtga
   106621 tatcgtcttc attactgtta ttggacaggt gctgatagca ttggggatca tggtagttac
   106681 ttttgctgga cgttggtttg catttccgcg tggcgtcatc gtgctctcgt ttatacttgg
   106741 cacgatatta cttagcgttt atcggatagt tgtttataaa ctatacttaa gagtcagtcg
   106801 tgataagaaa gtccttatcg taggtttgga gaaagatgtc gcaccagcta tccaaaactt
   106861 tcaatctaag aaaaacaaca aacataaagt tgaagcagta gtgatcgaca agtattacga
   106921 aaacacaaaa aaattgattg atgatatcga tattgtctac ttggcttcta aaatcccaga
   106981 agacgaaaaa atcaatattt atcaattagt aactaaaaaa gagaagaagc tatttttaaa
   107041 tacaactttt gaaaatttaa cgatgatcaa tccgaacatc atgaatttcg aagatgaaag
   107101 tatcattgaa gcttccggtt tcaaaattca gccggaatat gaattattca aacgtctttt
   107161 tgatatcatc gtttcgctta ttttactgat tcttgcttca ccttttatgc taatcacagc
   107221 gattttggta aaaacaactt cacctggacc aattatttat aaacaagtac gtattacgaa
   107281 aaatcaaaag gaattttcaa tctataaatt tcgttccatg agtgtaacag ctgaagcaaa
   107341 atcaggaccg gtcttggcaa agagcaatga tgcacgtgtg acaccagtag gcaaatttat
   107401 ccgtgccgta cgttttgacg aattacctca aatctttaat gtattgaaag gcgatatgtc
   107461 gatcgtcgga ccacgtccag aaagaccatt tttcgttgat cagttcaatg aagaaaatcc
   107521 ttattattat ttaagacaca atgtacgtgc agggattacc ggatatgcac aagtatacgg
   107581 aaaatatgta tcagattata atagtaaact acgttttgat ttgttgtata tcaaaaagta
   107641 ttccttacta ttagatttga agattttact tcaaacaatc aaaatcttat ttgataagat
   107701 gtcctcacaa ggactagatg aagatgaaaa tatccgttcg ttagcagaaa tcgacttttc
   107761 acaagagaaa atttatcgct agatgggaaa aattttgagc aaagaagtag gagagaacat
   107821 tggaaaataa aaatataagt atgctgcttt ctttatatga taaagaaaaa ccagagtatt
   107881 taaaggaagc attaactagt atttttgagc aaacagtaat gccaaaagaa attgtattag
   107941 tttatgacgg ccctataaat gaaaaacttc aagtagttgt cacttatttc caacaaaaag
   108001 atcctcactt gtttacagtt gtaaaattag aaaaaaatca aggattgggg attgctttag
   108061 caattggatt agaacatacg aaaaatcgct tagtagccag aatggataca gatgatatca
   108121 tggaacccca acgattagaa aaacaattag agatcttcca aaaagatcca gaaatagcga
   108181 ttgttgggag taatattgag gaatttactg gcacgcttag caatgttatc ggaagaagga
   108241 ttgtacctga atataatgat gaaatccgtg tgttttcgaa aagaagaaat ccctttaatc
   108301 atatgacagt catgtttgat aaaaatgcta ttttagaagt gggaaactat cgcccattaa
   108361 ttggatttga agattattat ttgtgggtaa gactgttgaa ggcgggatat aaagggtata
   108421 atatccaaga gattctagtc catgctagag caggtgctga catgtatgct agacgtggag
   108481 gaagtaaata tcttttacca ggaattaaag gccgatatcg cgtatggaaa gatggtctag
   108541 gatcactaaa agattttctg atagttattt gcggacatct tgtaattagt ttattaccga
   108601 ataaaattag gggaaaagta tatgaatcaa aacttagaaa aacaaactaa actttctata
   108661 atcatacctt tttacaatgc aaaaaatact ttaaaaagag ctgtagacag cgtcttaacg
   108721 caaggttatc ccaatattga aatcttgtta attgatgatg gatctacaga tggtagtagt
   108781 gacattggtg acgaaattgc taatctagag agtaaagtag catgttatca cttggaaaac
   108841 gctggtccat cagttgctag gaattgtggg ataaaagaag caactggaaa tttggtagga
   108901 tttttagata gtgacgatta ttttttacct gattgtttta ataatgtttt gaaagactac
   108961 cataggaatt accccgatat cattgctttt ggattaagta aaggaaaaaa tatagaaaat
   109021 gcaaatttat ttgttcccga aaagaagatt ggttgttcaa gtgaagaagc aattgagcaa
   109081 atgtttagaa gtaaggcagt ggatttttac gtatggaata aattttttaa aagggaatta
   109141 ttcaatcata tagtttttcc agaaaataaa ttatatgagg atatggttcc tatgtatgaa
   109201 gcttttagag cggctagtaa aattgatatt ttgccatttt caggtatttt ttattatcaa
   109261 aataatgata gcatcgttta tcagtctttt aattcaaaac agtatgataa tattttgcaa
   109321 agaaaaatat tagttgatga agtaaagaaa gattatcctc gacttattaa tttggccaat
   109381 gctcgattag tagacggata cctttcaaca gggtttaaaa ttactggcaa gaatcaaaat
   109441 cagaaagaaa agcaaaaata ttataatcag tctaaaagag aaataaaaag tatatattca
   109501 aaaataagaa gtaacaatga gatatcaatt gctaaaaaat tagctttatt tttatactta
   109561 attaatccta aaacttattt tattttatat aaaaaagttc taaaaaaata aagttttttt
   109621 cgatatgtta ttttttatgt ttattaactt cggaagacta aaattgttac tggtaatgat
   109681 acatttgtaa aaataaaaaa gatgattttt aggaagtgaa gagagtgcat aaaattagtg
   109741 ttatagtacc cgcatataac gtagaagact atttagaaaa ctgcttagaa tctatttcca
   109801 accagacaca ttcaaatatt gaaataatag tagtcaatga tggttccccg gatcgaagtg
   109861 gggaaattgc agaagaatat aaaaagaaag atatgcgatt aaaagtgata catcaagtga
   109921 atggtggttt aagtgcggct aggaattctg gtcttgattc tgcaacagga gattttgtgt
   109981 gctttattga cagcgatgat tggatagaac cagactatct tgagttacta tattttggca
   110041 cgcaaactta tggagcagat atctcggtgc taaaaataaa aaagatttca agtttagaac
   110101 aactaaaaga acatacaatt gctgagagaa aatgggaaat atttgataga gaacaagcaa
   110161 tgaaaaactt atttataaat aataaaattg gctattctgc aaataataaa ttattcaata
   110221 gaaatttatt tgataatata agattcccac tgggtaaatt aatggaagat aaggctacta
   110281 cttatttgct gatcgatcat tccgaaaaag ttgctgtcaa ttcatctgct aaatatcact
   110341 attaccaaag ccctaaaagt atactgagag gaagcttcaa tcctagaatt tttgattctt
   110401 ttgaagtcca tgaagatata ctaatattca tagataagtt ttatccggaa ttatcaatta
   110461 atgttagaac tcgctatgtc tatgaagcaa taaggatgat gatgacaatg attaaaaata
   110521 actataccaa taaagaagat tatttacgtt ctataaatat tttacaacgc tttgaaaaag
   110581 aagtgaaaat tgatcccgac ttttcaccaa aattaaaaaa attagtaaag tttttattaa
   110641 taaataaaaa cttgccttac tatatgtcaa gtagtaagat agcaagtaaa ttcttgatta
   110701 agatagaatc tgtgaggtaa gagatgaaga atataaaaaa atttttttca aggaatattt
   110761 tcccggtttt tattaaaata ttacctattc aaaaaaattt aattgtattt tcaagttttg
   110821 gcgggcgtaa ctattctgga aatccaagag ttatttcaga gaaaattcat gaaaaaaaat
   110881 tgaattacag aattatttgg ttaatcaaaa aagaagcgat tataactgat ttaccagatt
   110941 atattaaaag aattgactcc gattcttttt tagctgcctt ttatttatat acagctaagt
   111001 attggataga cgatagtcgg aaaacgatta agtatgcaaa aaggaaaaaa caactttatt
   111061 ttcaaacatg gcacggaacg cctttaaaaa aaattgaatt tgatgcaaaa gataagttac
   111121 ctaaacgtta cctaagctat gcgaaaaaag atagtgagtc aattacatat ttattatctg
   111181 gtaatcgcta tagttctaag aagtatgttt cagcattcaa tatcgttcct tctaaaatat
   111241 tagaagtagg aactcctaga aacgatgaat tagcctctag taaagagcaa gtgtttgact
   111301 tgaaaaagaa acaaatcaat gtcttatttg ctccaacttt tcgaaataat attgaggaca
   111361 acggaattac gcaactagaa tggttaggtg tggataactt acgtgaattt tttaaaaagc
   111421 aacaacaaga attaaatttt atgactaaat ttcatcctaa tgttcatagt aaattagcaa
   111481 ctgattctaa aagtatagaa tatatgaaaa aacatcaaat tactttaatt aatgatggga
   111541 ttcctcttga aatgcttttt gaagagatag atgttttaat cacagattat tcatctattt
   111601 ttttcgattt tgcgctaaca aaaaaaccaa ttattctttt taactatgat gaaaaagaat
   111661 atagcttaga aagaggtttt tatatacagt taagtgagtt accaattata agcgtacaag
   111721 atagttatgg ccttgttgat gtttttaaaa accaacaaga aaagcttttg aaaagttctc
   111781 aagagttact tgattatatt ggaaattatg aaacaggagc aagtacagaa aaggttcttg
   111841 agttgataga gagggatata aagtgaaaaa aacagtaaaa gatatatttt ataatgcgat
   111901 atatcaagtc tttttgattg ttttaccctt acttactata cctatactta gtcgtagaat
   111961 tggttcaact ggtttaggga tttatgggta tgttttctca atatcacagt ttttaatgac
   112021 tgtcattgct gtgggaatga atccttttcg tattcgaaat atcgctaaat caaggaaaga
   112081 taaaaaagca ctatctttac aattctggaa tatttacttt attcaatttt taataggctt
   112141 gagtgtttcc tttctctata tagcctatat ttattttttt gatgtaaagt ataaaaattt
   112201 atacttaatt caattgatat ttattgtagg gctaacatta gatatttctt ggttttttca
   112261 aggaattgaa gaattttcga aagtggttat tagaaataca gttatcaaat tatcatcggt
   112321 attgttaata atcatttttg tttcaactaa agatgatttg tggatttacg tgttcattac
   112381 ttcaattact aactttttag gaagtttagt tttctggtta agtatccacg gaaaagtagg
   112441 taaaccaatc tttaactcta atgtttttaa acagttgtgg aaaccagctt ttattatttt
   112501 gactcctcag ttgtttatgc aagtgtacac aactttagac aaaacgatag ttgggtattt
   112561 tgtggatcca acggaattat cttattatga tcagtctcaa aaaattgctc gtattatatt
   112621 ggcagtctta acatcaatta ctattgttat gttgcctaaa atatctaaag cacaggcaga
   112681 ggggaaatca gaaaatattt tagtttacac aaaaaaatct tttgattaca cgattatcat
   112741 tgcattaatt ctttttagtg tggtgtttat caatacaaaa gaatttgttc catggttttt
   112801 tggggagaat ttcaaaccga tgacaataaa catgcttatt tcttgtctaa ttatagttgt
   112861 tagtccaatt ggtggagttt ttgctaacca atttgcaatt gctatagaaa aagataagga
   112921 gtatgcttat ccaatgatca ttggtgcgat tgtaagtatt attggaaatg tagcttttgt
   112981 gcctacttat ggcgctgtag cggctacgat cgtactcgtg gtagtagaat tattagtctt
   113041 tttctttaga gtattttttg tacgaaaaga aataaaaata tcctatttct ttgggaagaa
   113101 cgttatctta ttttttatag tcaatcttac tttgactact tgggcctatt actttcttcc
   113161 acaattattc tcttcttcat ttttagatat ggcaattaaa tcaataatga ttttcattat
   113221 ttatcttccg tttgtttatt ttctttttcc agatatacga aatgaaataa gaaagattac
   113281 tcgaaaataa aataatgttg taaaaaagta gttcttt
//
LOCUS       DACGLE010000003        93453 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0534
            SAMEA3304449-rid9560453.denovo.003, whole genome shotgun sequence.
ACCESSION   DACGLE010000003 DACGLE010000000
VERSION     DACGLE010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304449
            Sequence Read Archive: ERR985064
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 93453)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 93453)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702225.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702225.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 20:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,045
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 27 of 67
            Pseudo Genes (incomplete)         :: 25 of 67
            Pseudo Genes (internal stop)      :: 19 of 67
            Pseudo Genes (multiple problems)  :: 4 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..93453
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304449-rid9560453.denovo.003"
                     /strain="VREN0534"
                     /db_xref="taxon:1352"
     gene            83..970
                     /locus_tag="IXT20_000247"
     CDS             83..970
                     /locus_tag="IXT20_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAQ4858725.1"
                     /translation="MQELKTRSARHHRPKRKMVINKKAIAPLIGTSLLVGPMMVTAVP
                     QHVHADEQGSQTVADPQAFIDQIGWSASEVAASNDLYASVMIAQALLESAYGTSGLAS
                     APNYNLFGVKGSYNGQVVYMATKEYVNGQWVTMNEPFRKYSSYWESFQDHANVLRSTS
                     FSTGDYHYAGVWKSNTTSFYDATAYLTGRYATDPNYGAKLNSLINTYNLTRFDTPSTG
                     SATTTTSTTTTTTTTTSTSSSSTTDVNTNNGQTYTVVSGDTLWDIAQKFGMSVDDLMA
                     KNGMSMSSYSLLVGQELNV"
     gene            1110..2036
                     /gene="cysK"
                     /locus_tag="IXT20_000248"
     CDS             1110..2036
                     /gene="cysK"
                     /locus_tag="IXT20_000248"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HAQ4858726.1"
                     /translation="MTAIYNSVTELIGKTPIVKLNKIVPEDSADVFVKLEFFNPGGSV
                     KDRIALSMIEKAEHDGLLKPGDTIIEPTSGNTGIGLSMVGVAKGYKVIIVMPETMSIE
                     RRLLMKGYGAELILTPGADGISGSIREAERLAKENGYFLPLQFENEANPLVHEKTTGP
                     EIHQAFGVNGLDAFVAGIGTGGTITGAGRELKRVYPKIELIGVEPAESAILEGKEAGP
                     HKIQGIGTGFVPKTLDTSVYDKVLSISGDEAMETAREVGRKEGILVGISSGAAIAAAL
                     KVAKELGKGKKVLAVVPDNGERYLSTALYQEI"
     gene            2272..2718
                     /locus_tag="IXT20_000249"
     CDS             2272..2718
                     /locus_tag="IXT20_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HAQ4858727.1"
                     /translation="MENKLVEEVIEKLKKENIRITPQRYAVLEYLIEHHSHPTADEIY
                     KALEHRFPNMSVATVYNNLRLFTSIGFVQEMKYGDASSRFDFSSKRHYHVICENCGKI
                     VDFHYPGLEDIEMAASRLTDFEINEHRLELYGLCPDCKESAKKAQM"
     gene            2991..4343
                     /locus_tag="IXT20_000250"
     CDS             2991..4343
                     /locus_tag="IXT20_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH dehydrogenase"
                     /protein_id="HAQ4858728.1"
                     /translation="MKVVVIGCTHAGTAAVKSILKNHPEAEVTVYERNDNISFLSCGI
                     ALYVGGVVKDPAGLFYSNPEELSSLGATVKMEHDVTDVDTENKTVTAKDLKTGETETV
                     SYDKLVMTTGSWPIIPPISGIDAKNVLLCKNYNQANEIIAQAKEAKRVVIVGGGYIGI
                     ELVEAFVESGKQVTLIDGLDRILNKYLDKPFTDILEKELTDRGVTLALGENVTEFITD
                     EEGKVKQVATPTDTFDTDMVILCVGFRPNTKLIEGKVDTLPNGAIKVNEYMQTSNPDI
                     FAAGDSAVVNYNPSGTQNYIPLATNAVRQGLLVGNNLTDHKMAYRGTQGTSGLYLFGW
                     TIGSTGVTKESATLNDLEVQATVFEDNYRPEFMPTTEKVMMELVYEKGTNRIVGAQFM
                     SKYDITQSANTMSLAVQNKMTVEDLALSDFFFQPHFDRPWNYLNLLAQAALGEIETAQ
                     "
     gene            4514..4801
                     /locus_tag="IXT20_000251"
     CDS             4514..4801
                     /locus_tag="IXT20_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4858729.1"
                     /translation="MNKEYFVQQDAFLEDYKSASKKLTINIDELADQVLSQLNETQEE
                     YYTMPANDAKDNVSHRFPFSRRIYSDENDGTLNTEYTYEGQPYVLETDDDL"
     gene            5027..5470
                     /locus_tag="IXT20_000252"
     CDS             5027..5470
                     /locus_tag="IXT20_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAQ4858730.1"
                     /translation="MDKRQLLIEEYLDLANEIIHRNKPEMEKEFAGFTLNELEVIEHI
                     GKIPDPNVTKLANASYMTRGAISKLTKKLIARGIINTYQSEENKKEIYFQLTPIGQKI
                     RNRHEQLHQKWVQRDATVFENMSKEEFDTVFRFIGRYREFIRSSK"
     gene            complement(5476..6660)
                     /locus_tag="IXT20_000253"
     CDS             complement(5476..6660)
                     /locus_tag="IXT20_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance MFS efflux pump"
                     /protein_id="HAQ4858731.1"
                     /translation="MYSFTNKHSKVFGYVSVLFTGLGFTIVSPVLPFLTLPYSHSIHQ
                     QAFYITLLMSVYALAAFLSAPILGSLSDHFGRHPILIISLLGSSIGYLLFGLGNSIWM
                     LFLGRIIEGLTAGEISTLYAYFADITEPNERTKVFGWMGALVGIGTTLGPIIGGLLAE
                     LGNSVPIFIGALFTFLNAVYGYTFMLESLPIKKRSVDLSFSHVRPFHQLQQLFKISSV
                     IPLLTAGFAVWLAAGSLQSIFSQFSIDAFQWKAGLVGLSFSLIGILDTVSQLFIMPRL
                     LKKFSEQQITRIGMFSEILAYLFITLSGILLLPILFLFGIICYGFGDSIFTPVFNGQL
                     SNSVSDNQQGLVMGGTQSIQSLSRVIGPLIAGQLYAAAPFLPTVFGFLLMIFAYYHYN
                     KK"
     gene            6928..7626
                     /locus_tag="IXT20_000254"
     CDS             6928..7626
                     /locus_tag="IXT20_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HAQ4858732.1"
                     /translation="MLNLENYLSAGQTVISNLLLKNYHRIGLQSEEFLFVLQLHMAQL
                     EGDTFPDLQMIAMDMGIKQDQIFQILDRLVTNGFIKIETTIVNGKKADRYNLYPIYDL
                     LGEYLKTQEKKYEEKTQEKEIQSVYQLFEQEFGRPLSSIEFQRIGQWLEEDHYQPEIL
                     KLALREAVLNQAYSFNYVDRILLSWERKNLRTKQQVEEDQKRRKQQLLQREVEQSAQQ
                     EELPKVSLKNWLEE"
     gene            7633..8310
                     /gene="nth"
                     /locus_tag="IXT20_000255"
     CDS             7633..8310
                     /gene="nth"
                     /locus_tag="IXT20_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HAQ4858733.1"
                     /translation="MLSKQKTMEALETMYGMFPEAHGELKHNNPFELLIAVILSAQAT
                     DVSVNKATPDLFASFPTPDALAEASIDEIILKIKTIGLYRNKAKNIKACAQQLIERFD
                     GQVPTSREELMSLPGVGRKTANVVLGDAFGIPAIAVDTHVERVSKRLRICKLDATVME
                     VEETLMRKVPQELWVKTHHTLIFFGRYHCTARNPKCEVCPLLSICQDGKNRMRLKEKA
                     LKKKPRL"
     gene            complement(8436..8969)
                     /locus_tag="IXT20_000256"
     CDS             complement(8436..8969)
                     /locus_tag="IXT20_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4858734.1"
                     /translation="MVLNNFLKEGFILSYLIVCLIIIICLHHLFLSKTIPRYSKNKKA
                     EKFCLLLNKFTLIAPILAFIIFSVLLSTTLKGKFMERSSHAMILTFLWLLFTRIYIFL
                     MSLKPPKSISLCLVINGIFLLSLIIFITPLDRYVTYLYNPLEYWTYFIGILEGIIFYI
                     GYFPNKNNNFYFYRNKL"
     gene            complement(9266..11647)
                     /locus_tag="IXT20_000257"
     CDS             complement(9266..11647)
                     /locus_tag="IXT20_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HAQ4858735.1"
                     /translation="MANEQTRSSRRQKQPTPKKSVKKNSGKGSGKSSGTHKKGLFIKI
                     LLGILSFFCILFLAGVGLFWYYAKDAPELTDKKLDATVSSKLYTQDGELFEDLGAEKR
                     EKISANELPKTLEDAIVSVEDRRFYKHIGVDPIRIIGSALSNFTSGGLQGGSTLTQQL
                     IKLSFFSTSAEDQTLKRKAQEAWMAVRLEQKKSKQEILTYYVNKVYMSNGLYGMETAS
                     EMYFGKKLSELSLPQTALLAGMPQAPSAYDPYVYPDQAKKRRDTVLYTMLQNEKISQT
                     EYDQAVNVPVTDGLQELTQSDDNTKIVDNYVKEVINEVQEKTDKNVYTDGLEIYTNLD
                     LDAQKKLYDIVNTDQYVSYPDDEMQVASTLIDTNTGKVKAQIGGRHIAEDVTLGNNLA
                     VNTSRDFGSTMKPVTDYGPAFEYLKYSTGKTITDAPYNYEGTSTPVGNWDNQYMGTIT
                     LRQALYLSRNVPAVKLFNEVGSDKVASFLKNLGIEYSTIHQSNAISSNTEEQDGTKYG
                     ASSLKMAAAYAAFANGGTYYKPQYVNKIVFQDGTEETYEPDGKTAMSPETAYMITDIL
                     KDTITEGTGTNAQIAGLYQAGKTGTSNYTDDEYAKLGISSGVYPDILFAGYTPNYSIS
                     VWTGYNKKMTPVTSESSHVASDVYRELMQYVSANVTNTDWEMPSGLIRVGGELYYKDQ
                     YTARSNAITPSTTIPSSSYVQTPGSSTTETTTQSSSSTSQSESTAESSKESTTAETSE
                     PASSTTVPSSSSEESSTPSSSAPPASSSEPATSGADAANDHTPSSSTSASGNR"
     gene            complement(11637..12284)
                     /gene="recU"
                     /locus_tag="IXT20_000258"
     CDS             complement(11637..12284)
                     /gene="recU"
                     /locus_tag="IXT20_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HAQ4858736.1"
                     /translation="MVLRYPNGQPYYKNASPVEKIQEKRNQITEFGNRGMRFEEAINE
                     SNKYYLSRGLAVIHKKPTPIQIVKVDYPKRSAAVIKEAYFTQASTTDYNGVYRGFYLD
                     FEAKETKNKTSFPFKNFHAHQIEHMKACLEQNGVCFVLLWFSSLKRCFYLNSVHLIAY
                     WDAQKEKGRKSLPLSLIEELGIEIKTGISPRIPYLDAVDFYLSTLSIEGESKNGK"
     gene            12348..12887
                     /locus_tag="IXT20_000259"
     CDS             12348..12887
                     /locus_tag="IXT20_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1273 domain-containing protein"
                     /protein_id="HAQ4858737.1"
                     /translation="MDKSKVIYVSGYRSFELGVFKENDPKIQVVKNVLKKELHQLAEE
                     GLEWVLVSGNLGVELWAVQIVAELKEEYPELSLGILYPFSDFGNNWNEGNQEKKQLAE
                     QLADYVEAVSHQPYQSPSQLKNHTRFILEHTVGCLLIYDEEFPGKTKYFFEDAQNYQA
                     DHPYEIRLISMDDLQNFSE"
     gene            12977..13396
                     /gene="gpsB"
                     /locus_tag="IXT20_000260"
     CDS             12977..13396
                     /gene="gpsB"
                     /locus_tag="IXT20_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division regulator GpsB"
                     /protein_id="HAQ4858738.1"
                     /translation="MANLVYSPKDILQQEFKTKMRGYDPVEVDEFLDNIIKDYETYSK
                     ELLALQEENDRLSAKVAQLSKTQGAAQTRVQQTEAPKSAAVTNFDILKRLSNLEREVF
                     GKKLDQQASAVKPAQPNPNNYTNADTSLDDNEKTRQF"
     gene            13418..13785
                     /gene="rnpB"
                     /locus_tag="IXT20_000261"
     ncRNA           13418..13785
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="IXT20_000261"
                     /product="RNase P RNA component class B"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00011"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00011"
     gene            13994..15160
                     /locus_tag="IXT20_000262"
     CDS             13994..15160
                     /locus_tag="IXT20_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HAQ4858739.1"
                     /translation="MEKNRKFNLVATAASGLEALVGKELRDLGIECQVENGRARFEGT
                     METIATANLWLRTADRVKIVVGEFDAYTFDELFENVKALPWEDFLPLDAAFPVAGKSI
                     KSKLYSTPDCQAITKKAIVERLRTYYHRPASVPLTETGAHFQLEVALLKDHVMVTLDT
                     TGPSLFKRGYRLEKGGAPLKENMAAALIMLTNWRKDRPFYDPVCGSGTLCIEAALIGH
                     NIAPGFNREFACESWDWFSQEIMENVRSAAEEKADYDIELDITGSDINGRMIEIAKAN
                     AEEIGLGSSITFKQQAVKDFKTDKEYGVIVANPPYGERLGEEETVRKLYKEMGDVFRP
                     LKTWSKYILTSDLAFEEFYGQKATKKRKLYNGALRTDLFQYWGTRPPRKKEKDE"
     gene            15206..16702
                     /locus_tag="IXT20_000263"
     CDS             15206..16702
                     /locus_tag="IXT20_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HAQ4858740.1"
                     /translation="MKEKEFLQEVKEINLLQQALGILDWDTQTGMPEKASAYRSEVDS
                     YLYSIYFSKKIGPKIKEAIRYFSEHPEELSEVGTAVFAKVKEEYDLEHRVPEDLMRKY
                     TAAVSSAHGQWQKARETQQFSDFQHALNQNIELTKQLIPYWRKDEKTNYDVLLNQYEP
                     GMTVEILDKVFDQLKEGILSIRRTLQEKGTEPETDFLNRRMTKKQQKRFVVKVIEQLG
                     YDFSRGRLDDTVHPFMTGINPNDARITTRWNETDFKMAVFGVIHEAGHGMYEQDIDDK
                     YAYTPVYEGTSMGIHESQSLFNEIIIGSSRSFWKKQYPFFQECAEGTFDDIPFDKFYR
                     SLNHTKSSLVRIEADSLTYPLHIIIRYEIEKMIFNEGVDVAELPEIWNQKYEEYLGIR
                     PTNDVEGILQDVHWSGASFGYFPSYALGYMYAAQLLHSLQQEIDVENVLASDDYSPIK
                     NWMSEHIHQYGASRKPNQLIMDATHEPLNPQYLIDYMKDLYFNVYQVK"
     gene            16783..17466
                     /locus_tag="IXT20_000264"
     CDS             16783..17466
                     /locus_tag="IXT20_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAQ4858741.1"
                     /translation="MDKLQVIADYSMDQLAHDQTGHGSDHTKRVVKLAEKILETEPQA
                     DRFVTLAAAYLHDTIDDKVVKNENEAKQQLRVFLRSLPIVEEQISMIFSIIENMSFSK
                     NLSEAAELSLEGKIVQDADRIEALGAIGILRTAYFGGGHGHPIFDPELYPQTFKDKEN
                     YRKGTTVINHFYEKLFLLSDKMNTDYGYEEAKRRETFMREFLEEFFLEWSIDDENFTP
                     DNWIKNNKK"
     gene            18111..19301
                     /locus_tag="IXT20_000265"
     CDS             18111..19301
                     /locus_tag="IXT20_000265"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HAQ4858742.1"
                     /translation="MSSKELTMYLEKELDQLKEKGLYNTIDILESENGACIIVDGKKM
                     INLASNNYLGFANREELKKACIEATETYGVGAGAVRTINGSLKIHQQLEEKIAEFKGT
                     EAAIAFQSGFNCNMGAISAVMTKEDAILSDELNHASIIDGCRLSGAKIIRIKHQDMKD
                     LEKKAKEAIESKKYKKIMYITDGVFSMDGDIARLPEIIPIVEKYGLITYVDDAHGSGV
                     TGKGAGTVKHFGLSDKIDMQMGTLSKAIGVVGGYVAGSKTLIDWLKARSRPFLFSTSL
                     TPGAAAAALASITLMQEHPELVEKVWENANYFKEELKKVGYNIGMSETPITPVILGDE
                     KVTQTFSKKLIEHGIYAKPIVYPTVPLGTGRIRNMPTAEHTREMLDEAVAVYQKIGKE
                     MEII"
     gene            19403..20356
                     /locus_tag="IXT20_000266"
     CDS             19403..20356
                     /locus_tag="IXT20_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HAQ4858743.1"
                     /translation="MKKVLVTGCLGQIGSELITRLRQELGSENVIATDIRMPEVIEKN
                     DIFEILDVMDYEKMRSIAEQYQVDTLIHLAALLSAVAEAKPQLAWNLNVTGLVNALEV
                     AREFQLKFFTPSSIGAFGPNTPKDHTPQDTLQRPTTMYGVTKVAGELLCDYYYVKYGV
                     DTRGVRFPGLISYATLPGGGTTDYAVDIYYSAIKEGKYTSFIDQGTSMDMMYMPDAID
                     AIIKLMNADPDRLVHRNAFNISAMSFDPEEIKASIQKIIPDFEMDYDVDPVRQSIAES
                     WPNSLDVSCAQEEWGFSPKYDLDVMTKDMLEKLKEKLVETV"
     gene            20425..21774
                     /locus_tag="IXT20_000267"
     CDS             20425..21774
                     /locus_tag="IXT20_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ4858744.1"
                     /translation="MEEYQATPVKEVKIENELKRTMGFFTALSTVMGTVIGAGVFFKA
                     ASVAEVTGSASLHMFSWFLGGMISVCAGLTGAELAAAIPETGGMIKYIERIYGNTAAF
                     LLGWAQVVIYFPANVAALSIIFGTQFVNLFGLSQSMIVPVAVTAAVSILLINFLGSKA
                     GGAFQSITLVCKLIPLFVIVIFGLFRQEGVDFQLFPIQAGENLSFFSALGAGLLATMF
                     AYDGWIHVGNISGELKKPAKDLPKAISLGIIGIMIVYLLVNAVFLRTASIDGVAGNSN
                     AASDVAKMIFGGFGGRLVTVGILISVYGTINGYTLTGMRLPYVMAKENNLPFSKLFAK
                     LHDKTKVPVAAGILELVIAIGMMMIGGFDTLTDMLIFVIWIFYTMVFVGVILLRKKEP
                     DLFRPYKVPMYPFIPLVAIIGGTFIVSSTLITQTFLASMGIVLTLAGIPIYLYLKRR"
     gene            complement(21957..22280)
                     /locus_tag="IXT20_000268"
     CDS             complement(21957..22280)
                     /locus_tag="IXT20_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4858745.1"
                     /translation="MLNMYFVFGVPIFLLFLYATIAYVRKRTTIHYLGFILLIISGFM
                     LVFNLQTWQQALLEMDKMTPHALSKVLGYPVYLIWLPIFISGCLVLLNIYRGVRRIVQ
                     LRKSK"
     gene            22409..23572
                     /locus_tag="IXT20_000269"
     CDS             22409..23572
                     /locus_tag="IXT20_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4858746.1"
                     /translation="MASYIEPIRFALIVFPFLALAISSVFFIYEYRKYGTFLVTRAVI
                     LYSFVFYLLCAYFLVILPLPAKETVAQMTGPKMELHLFASWQHFRDNTVLVLSEPSTY
                     LPAMKQSVFLEPAFNVLLLIPFGIYLRYYFRLSFMKTMIASFCLSLFFELTQLSGLYF
                     IYPRPYRLFDVNDLVTNTLGGIIGFVVTPLFTFMLPTRRRMDELSYEKGKTVSLTRRL
                     VAYLIDWTFLSLINLVAAIFFKIAFDSGAVTSHIWWLLLEVGVYFMIVPFITNGWTLG
                     KKIVRIRLVEEDRERISLKALMIRYGYLYFLFYGLSWLGQLLQQIMNSTTNTVLQMSA
                     VFISLGVLLLQGIFILNILLSFLRKKRILFYEKASHTYTVSTIGQTKDDDSRN"
     gene            23642..24991
                     /locus_tag="IXT20_000270"
     CDS             23642..24991
                     /locus_tag="IXT20_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HAQ4858747.1"
                     /translation="MNNFDIFRFKPFINEALTAKGFKQPTEVQERLIPLIAKGKNIIG
                     QSQTGTGKTHTFLLPLFEKINAEKEEVQVVITAPSRELAAQIYEAATQIAKFSTPEIR
                     VSNFVGGTDKQRQLNRLHHQQPQVVVGTPGRILDMMNEQALKIHTAFAFVVDEADMTL
                     DMVFLNEVDQIASRLPEKLQFLVFSATIPEKLRPFLRKYMENPIIEEIKPKAVISEDI
                     ENWLISTKGKDENQMIYQLLTIGHPYLAIVFANTKQHVDEIADYLKNQGLKVAKIHGD
                     ITPRERKRVMRQVQNLEYQYVVATDLAARGIDIEGVSHVINAEIPEDLDFFIHRVGRT
                     GRNGLKGTAITLYSPNDEQAIDAIEKLGVSFEPKEIKNGEIVKTYDRNRRAKREKSKD
                     VLDPTLIGLVKKKKKKIKPGYKKKIDWAIAEKNKKERKIERRQQSRTARKNKKNSHK"
     gene            complement(25036..25467)
                     /locus_tag="IXT20_000271"
     CDS             complement(25036..25467)
                     /locus_tag="IXT20_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ4858748.1"
                     /translation="MKKIVGADEWIRAASCFLRYQVFVLEQGIPAELEFDGKDIPQQT
                     YYLFLDQKRPVATLRYEKKTPTCFKPERFCVAKEYRGKGLGGQLLEAAEKRAVSEGCQ
                     TSYVVAEMTAVDFYEKQGYHTCTEPFIEDEVLCIGMKKSLA"
     gene            25855..28497
                     /gene="alaS"
                     /locus_tag="IXT20_000272"
     CDS             25855..28497
                     /gene="alaS"
                     /locus_tag="IXT20_000272"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HAQ4858749.1"
                     /translation="MKQLSSAQVRQMFLDFFQSKGHTIEPSASLIPVNDPTLLWINSG
                     VATLKKYFDGSVVPENPRITNAQKSIRTNDIENVGKTARHHTMFEMLGNFSIGDYFKN
                     EAIHWAWEFLTDPKWMAFDPEKLYVTVYPKDTEAKRIWHEEVGLPLDHIIDVEDNFWD
                     IGAGPCGPDTEIFYDRGEAYNDLAEDDPENYPGGENERYLEIWNLVFSEFNHKPDNTF
                     EPLPHKNIDTGMGLERMVSIVQDASTNFETDLFMPIIHAVEKLSKQVKYGENPVTDIS
                     YKVIADHIRALSFAIGDGALPSNEGRGYVLRRLLRRAVMHGKKLGIEQAFLYRLVPVV
                     GEIMVSYYPEVLQQKEFIEKVIRTEEERFNETINEGLEILNQVIQEVKNENGTTLAGK
                     DIFKLYDTYGFPVELTEEVAEDEGLKVDHAGFEKEMDAQRERARSARSTEKSMGVQSA
                     LLTDIKVESKFIGYTEQESDSQLLVIVKGESLLNEVAEGEAQMIFDQTPFYAEMGGQV
                     ADQGWIMNEVGEIVAVVTDVQKAPNGQFMHTVEVKAPMSEGQTYHLLIDQSMRNRIIK
                     NHTATHLLHKALKEVLGEHANQAGSLVAPGHLRFDFTHFGQITSDELAEMERIVNEKI
                     WEAIPVETIETDIDTAKNMGAMALFGEKYGHDVRVVNIGGWSIELCGGTHVANTENIG
                     IFKIVSESGIGAGVRRIEAVTSKEAYELLEEEEKQLKQVATIVKSPQLKEVVSKAEQL
                     QQQVRELQKENEQLASKLANQQAGDVFKDPETINGKTIIAAQVKVKDMNQLRQLADQW
                     KQKNISDILVLANEQEGKVSLLAAMTKESNEAGLKAGDLIKAIAPKVGGGGGGRPDMA
                     QAGGKNPAGIPDALKAAKEWVAQQ"
     gene            28697..29293
                     /locus_tag="IXT20_000273"
     CDS             28697..29293
                     /locus_tag="IXT20_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAQ4858750.1"
                     /translation="MNSRNKTFRLVLRAILLAIIIVQAMVPWLGFIPLGFISLTIIHI
                     TVIIAAVVLGPKDGMVIGLFWGIATIVRAYAMPTTPFDTLVFTNPIISVVPRVLVGLV
                     AGLVFHWIYQKNKSITISSAFAGVLGSLVNTVLVLGFMGLFYTGATADAYGVDSALLF
                     KTLAGVAAINGIPEAIGAGIITPLLAKALFAATPLKPE"
     gene            29415..30563
                     /locus_tag="IXT20_000274"
     CDS             29415..30563
                     /locus_tag="IXT20_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HAQ4858751.1"
                     /translation="MAKKQEEKKKQIPQQRTKKGVLAGILGLVTNLILFVSKLMIGLF
                     SGSVSIMADAVNSLSDTASSILTLIGFKIAAKPADQEHPYGHERFEYISGLFVSIIIT
                     YVGFQFLESSAEKIIHPENVSLTPIVFFVLIISIFLKFAQGKMYQVIAQSIDSETLRA
                     TATDSYNDVFTTLAVLLSAAIEWVTGWRIDGYIGFALALYILYSGLTMLRNFVYELLG
                     SRPTPAEIEEMEEHLNACDDILGYHDLLVHNYGPNKRFASVHIEVDESMNLNVAHKII
                     DLIEKDFKKSLDVELVCHLDPMPVDNEKYYNILRQLKQILLETGTGLNIHDFRVIHQG
                     KTLQFDVVVPEKFQFVDTELERILRSQIEEKIGDYQLEITFDHNYLLY"
     gene            complement(30601..31014)
                     /locus_tag="IXT20_000275"
     CDS             complement(30601..31014)
                     /locus_tag="IXT20_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HAQ4858752.1"
                     /translation="MLKVYIDASTKGNPGPSGGGILIVYQGKQEQLSIPLSIGTNHQA
                     EFEVFLKTLEFLKKNDLQNETIFCFSDSKTLVSTVDKNATKNELFLPYLQRIQELLSE
                     FSLLILQWLPENKNKGADNLARQALHKQLKEKKTL"
     gene            31093..31524
                     /locus_tag="IXT20_000276"
     CDS             31093..31524
                     /locus_tag="IXT20_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EbsA family protein"
                     /protein_id="HAQ4858753.1"
                     /translation="MKKNIRWQPELAHTIIYWSLTLIILFFSLILSLENTGPYWKSNF
                     VLGIFLVFLFLGLRRSFILKSNSVIIRYAAFWKDREISVGQIRSLTPTNTGVQMMLQD
                     EKAPSVFMMRKKDQVVLFDHFEKNGPKSESIPYPYTKKRKS"
     gene            complement(31623..31856)
                     /locus_tag="IXT20_000277"
     CDS             complement(31623..31856)
                     /locus_tag="IXT20_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HAQ4858754.1"
                     /translation="MTTGIVKWFDNKKGYGFISYDDTEEIFVHFTAIEEEGFKTLEEN
                     QVVEFEIIEGNRGTQAAHVKKGNVQTTDTSMHE"
     gene            32039..33709
                     /locus_tag="IXT20_000278"
     CDS             32039..33709
                     /locus_tag="IXT20_000278"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HAQ4858755.1"
                     /translation="MKNDLQISQETELEPIVEVASKLDLKEDDLELYGKYKAKINFSG
                     INRSKDNAEGHLILVTSINPTPAGEGKSTITVGLGDALNKINKKSVIALREPSLGPVM
                     GIKGGAAGGGYAQVLPMEDINLHFTGDMHAITTANNALSALLDNHIHQGNELNIDARR
                     VIWKRVVDLNDRELRKVIVGLGGPIQGVPREDGFDITVASEIMAILCLAADLEDLKAR
                     LARIVVGYTFDRQPVTAGDLKAEGALALLLKDAIKPNLVQTIYGTPAFVHGGPFANIA
                     HGCNSILATKTALRLADYVVTEAGFGADLGGEKFLDIKVPNLKKAPDAVVIVATVRAL
                     KMHGGMKKDELKNENLDALKVGFANLKRHIRNMEQYQLPVIVAINEFVTDTDNELTLL
                     EHLCENQGILAKRASVWADGAEGGVDLAKAVVRLIDRKEANYKPLYRLEETIQEKTET
                     IVKKIYGGNGVVFSKKAQKQIEEFTKNGWDKLPICMAKTQYSFSDDPSLLGAPEDFTI
                     TIREIIPKLGAGFLVALTGDVMTMPGLPKKPAALNMDVTNDGEVLGLF"
     gene            33856..34242
                     /locus_tag="IXT20_000279"
     CDS             33856..34242
                     /locus_tag="IXT20_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4858756.1"
                     /translation="MKTTFIRKTGFYGMGSSIQLRINNEKRSVLSHEQKITLDLEVPF
                     TMQVTFYWLKSPVYTVKEAKPAYVITMNYLILQIYPFLFLATGLGAVFLQNLFFSFFA
                     VIVLIAFFFYIKNRAYLIKEENYGKL"
     gene            34229..34927
                     /locus_tag="IXT20_000280"
     CDS             34229..34927
                     /locus_tag="IXT20_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HAQ4858757.1"
                     /translation="MANSDLFLISFNRIEKWMRDEMGNARNMGFTELVRRLAQRKQLM
                     IRKYEDDLLQLAQLRNAIVHDRIAVDFVIAEPNDWTVKRIQLIEQELIQPETVLPRFA
                     KRVTGFEQDLPLLELLKIVAEKRYSQFPLYNKGKFVALITLRNIGFWLAKESQKGPVD
                     LTNKRALDLIIQNGKYTNYHFVPAKTHIYEVEAMFREQGTLEAVLITKDGNPDGNLLG
                     IVRPRDIYKQIEKD"
     gene            34932..35396
                     /locus_tag="IXT20_000281"
     CDS             34932..35396
                     /locus_tag="IXT20_000281"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HAQ4858758.1"
                     /translation="MLIIYWIFSALLVGVDQLVKWWITDNLALGETKNLIPGILSLNH
                     IRNTGAAWSMLEGKMWFFTIVTLIAVVVILTLMIKNREKGNRWFMIGLSLILAGAIGN
                     FIDRIRLGYVVDMFQTDFMSFPIFNVADVTLVCGVICMLIYIILDEKDQRKK"
     gene            35393..36304
                     /locus_tag="IXT20_000282"
     CDS             35393..36304
                     /locus_tag="IXT20_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HAQ4858759.1"
                     /translation="MTELKTTIHHESGRIDKVLTNLFTDYSRSQIQLWLKNGAVSVNG
                     QPVKANYKVKQGDEITVLIPEPETLDVVPENIPLTIVYEDEDVAVINKPQGMVVHPSA
                     GHTHGTMVNALMYHIKDLSTINGVIRPGIVHRIDKDTSGLLMVAKNDQAHESLAQQLK
                     DKTSLRKYVALVHGEIPHEKGTINAPIGRSKENRKMQAIREDGKSAVTHFTVLERFEG
                     FTLVELKLETGRTHQIRVHMKYIGYPLAGDPVYGPKKTLKGNGQFLHAQMLGFTHPTT
                     NEKMIFEAPLPEVFEKTLEKLRKNIAF"
     gene            36539..37078
                     /gene="pyrR"
                     /locus_tag="IXT20_000283"
     CDS             36539..37078
                     /gene="pyrR"
                     /locus_tag="IXT20_000283"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015694870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HAQ4858760.1"
                     /translation="MQAKEVVDQVTMKRALTRITYEIIERNHSIQDIVLVGIKTRGIY
                     IASRIAERLKQLEDIDIPVGELDITLYRDDKKENPEEPELHSSDIPVSLEGKEVILID
                     DVLYTGRTIRAAMDAVMDFGRPRKISLAVLVDRGHRELPIRADYVGKNIPTSRAEEIL
                     VEMQELDGQDRIMILKEED"
     gene            37092..38375
                     /locus_tag="IXT20_000284"
     CDS             37092..38375
                     /locus_tag="IXT20_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HAQ4858761.1"
                     /translation="MSEKKGFHNDDVVLDIHDRPTPGHWVGLSLQHLFTMFGATVLVP
                     ILVGIDPGIALVSSGLGTLVYLFTTKGKIPAYLGSSFAFIAAMQLLMKTDGYPAIAQG
                     AVTTGLVYLIVALIIKKIGSAWLDKILPPIVVGPVIMVIGLGLAANAATNAMYNQGTY
                     DFKYIAVALITLGLTIFFNMWLRGFLGLIPILLGIISGYIVALLFGIVDTKPIVDAAW
                     FAVPNFEIPFVQYEPKLYLGAITTMAPIAFVTMTEHIGHLMVLNKLTKRNFFEEPGLH
                     KTLTGDGLAQIVAAFVGGPPVTSYGENIGVLAITRVHSVFVIGGAAVFAVILGFVGKL
                     SALILSIPGPVISGISFILFGVIAASGMKILIENKIDFDKKKNLLIASVILVVGIGGL
                     VFEVGTFTLSAMALATVLGIILNLILPETSRSEEN"
     gene            38388..39314
                     /locus_tag="IXT20_000285"
     CDS             38388..39314
                     /locus_tag="IXT20_000285"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HAQ4858762.1"
                     /translation="MIIKSERISLKHLLTVEALTDQEVMGLIRRGQEFKQGANWTPQK
                     RQYFATNLFFENSTRTHKSFDVAEKKLGLEVIEFEASTSSVQKGETLYDTVLTMSALG
                     VDVAVIRHGDENYYDELIQSKTIQCSIINGGDGSGQHPTQCLLDLMTIYEEFGHFEGL
                     KVAIVGDITHSRVAKSNMQMLKRLGAQVFFSGPREWYDDEFEVYGHYMPLDELLDQVD
                     VMMMLRVQHERHDGKESFSKEGYHQEHGLTVERAKKLQKHAIIMHPAPVNRDVELADS
                     LVEGLQSRIVQQMSNGVFVRMAILEAVLNGKA"
     gene            39333..40613
                     /locus_tag="IXT20_000286"
     CDS             39333..40613
                     /locus_tag="IXT20_000286"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HAQ4858763.1"
                     /translation="MKTLIKNGQINTRKNMTTPAEIWIEDGRIKAIGTGFSEAEFDEV
                     FDAKGQLITPGLVDVHVHLREPGFTYKETIEAGTRSAARGGFTTVCAMPNLNPVPDTA
                     EKLRQVYDIIRKDAVVKVLQYAPITENLRSEKLVDQEALIEEGAFAFTNDGVGVQTAG
                     TMYLAMKEAAKNNKALVAHTEDESLLFGGVMHAGKKAEELGLPGILSVTESSQIARDL
                     LLAEATGVHYHVCHVSTKESVRVIRDAKKAGIHVTAEVSPHHLILIDEDIPEDFGFWK
                     MNPPLRGREDREALIEGLLDGTIDCIATDHAPHGLEEKSQSFMKSPFGIVGSETAFQL
                     IYTHFVETGRFTLEQVINWLAVKPAEIFGLNAGTLTVGAPADVAVFDITQTCTIDKED
                     FLSKGENTPFIGWKVKGETQMTFVNGKLVWQKGE"
     gene            40618..41694
                     /gene="carA"
                     /locus_tag="IXT20_000287"
     CDS             40618..41694
                     /gene="carA"
                     /locus_tag="IXT20_000287"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010744830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HAQ4858764.1"
                     /translation="MERLLILEDGTVFEGKAFGADASVFGEIVFTTSMTGYQETITDQ
                     SFNGQIITFTYPMVGNYGVNRDDYESISPTCKGVVVKEHARVASNWRNQMTLDSFLKQ
                     KGIPGISGIDTRALTRKIREVGTMKASIVDAGDSFEHAFDQLKATVLATNQVQQVSTN
                     KPYPSPGTGRKVIVIDFGLKHSILRELSKRNCNLTVLPYNTDAETILALSPDGVMLTN
                     GPGDPKDVPEAIEMIKKIQGKVPIFGICLGHQLFALANGADTYKMKFGHRGLNHPVRE
                     VATGRIDFTSQNHGYAVDEATIDPEKLIVTHVEVNDGTVEGVKHRDYPAFSVQFHPDA
                     APGPHDALHLFDEFMEMMDAGKEQ"
     gene            41699..44884
                     /gene="carB"
                     /locus_tag="IXT20_000288"
     CDS             41699..44884
                     /gene="carB"
                     /locus_tag="IXT20_000288"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HAQ4858765.1"
                     /translation="MPKRTDIKKIMVIGSGPIIIGQAAEFDYAGTQACLALKEEGYEV
                     VLVNSNPATIMTDKEVADQVYIEPITLEFVSRILRKERPDALLPTLGGQTGLNMAMEL
                     AASGILEELNIELLGTKLSAIDQAEDRDLFKQLMKELNQPIPESEIVNTVEEAVDFAN
                     KIGYPVIVRPAFTLGGTGGGMCENEEELRIVSENGLSLSPATQCLIERSIAGFKEIEY
                     EVMRDSADNAIVVCNMENFDPVGIHTGDSIVFAPSQTLSDHEYQMLRDASLSIIRALK
                     IEGGCNVQLALDPHSFNYYVIEVNPRVSRSSALASKATGYPIAKLAAKIALGLTLDEM
                     KNPVTGTTFAEFEPALDYVVAKIPRWPFDKFENGERLLGTQMKATGEVMAIGRNIEES
                     LLKAVRSLEIGTHHIELPELADITDDDLIQKVVRAQDDRLFYVAEAIRRGYTIEELAD
                     LTKIDLFFLDKLLHIVEIEQQLKQQPLDDKILKTAKQNGFTDKKVAELWEINEEAVRQ
                     MRKENDLIPVYKMVDTCAAEFESKTPYFYSSYEYENESSRSEKPSILVLGSGPIRIGQ
                     GVEFDYATVHSVKAIQQAGYEAIIMNSNPETVSTDFSISDKLYFEPLTFEDVMNVIEL
                     EQPIGVIVQFGGQTAINLAEPLTAAGVKILGTTIEDLDRAENRDLFEQALKELDIPQP
                     LGDTATSKEEAVVIASRIGYPVLVRPSYVLGGRAMEIVENQRDLEDYMEHAVKASPEH
                     PVLVDRYLLGGECEVDAICDGETVLIPGIMEHIERAGVHSGDSMAVYPPQTLSESIKK
                     TIEDYTIRLALGLNCVGMMNIQFVIHENEVYVIEVNPRASRTVPFLSKVTGIPMAQIA
                     TKAILGEKLKTLGYENGLYPETKEVHVKAPVFSFTKLQKVDTYLGPEMKSTGEVMGSD
                     RQLDKALYKAFEASGLHLPEYGAVLFTIADETKEEALELAKRFTEIGYSLIGTKHTAA
                     YFEKNGLVVETVAKISEEAAEKNVVDLIRDGKTQVVVNTIDKDRGNASKDGFIIRREA
                     VEHGIPLFTSLDTADAIVRVMESRAFSTNPI"
     gene            44938..45714
                     /locus_tag="IXT20_000289"
     CDS             44938..45714
                     /locus_tag="IXT20_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HAQ4858766.1"
                     /translation="MKQEMMEIVQQKQLAPKIFEMTLKGDLVKEMQQPGQFLHILVPR
                     ADLLLRRPISLNHIDHETNTCRIIYRVEGEGTRVFSTLSAGDHLDVMGPLGNGFDLSN
                     LHTGDEVFIIGGGIGVPPLYELSKQLVEKGIKPTHFLGFATHEVVYYEKEFNRLGETR
                     IATDDGTYGMHGNVGNLLLGTKTEPTAVFACGSNGLLKTVEQLFSSHQNVQLSLESRM
                     ACGIGACYACVCHTPEDETGTKSVKVCDEGPIFKIGEVII"
     gene            45711..46643
                     /locus_tag="IXT20_000290"
     CDS             45711..46643
                     /locus_tag="IXT20_000290"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HAQ4858767.1"
                     /translation="MNENPLAVKLPGLELKNPIMPASGCFGFGKEYGKYYDLNQLGSI
                     MVKATTPNARFGNPTPRVAETPSGMLNAIGLQNPGLDVVMHKLLPELEEYNELPIIAN
                     VAGACEEDYVEVCSKIGEAPNVKAIELNISCPNVKHGGIAFGTDSDVAFQLTQAVKKV
                     SSVPVYVKLSPNVTDIVPIAQAIEAGGADGFTMINTLLGMRIDLKTRKPILANQTGGL
                     SGPAIKPVAIRLIHQVASISSLPIIGMGGVQTVDDVLEMFMAGASAVAIGTANFTDPY
                     ICPKLIKELPVRMSELGIESLERLIKEVREERER"
     gene            46640..47356
                     /gene="pyrF"
                     /locus_tag="IXT20_000291"
     CDS             46640..47356
                     /gene="pyrF"
                     /locus_tag="IXT20_000291"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HAQ4858768.1"
                     /translation="MNQLDQRPIIALDFSTRQEVEDFLRFFPKEEKLFVKIGMELFYQ
                     EGPEIVRYLKEAGHNVFLDLKLHDIPNTVEKAMRGLAKLGVDLTCVHAAGGIRMMEAA
                     MRGLEEGTPEGGKRPLLLAITQLTSTSEEEMHADQLIEVSLEKSVIHYASCAKKAGLD
                     GVVSSAWEVEAIKEMAGDEFVCLTPGIRPEGTVAGDQTRVVTPSQAKKIGSTFIVIGR
                     PITQSTNPYKAYQTIQTEWS"
     gene            47363..47992
                     /locus_tag="IXT20_000292"
     CDS             47363..47992
                     /locus_tag="IXT20_000292"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HAQ4858769.1"
                     /translation="MNVEQSIAKDLLEIEAVFLNPSNPFTWASGIKSPIYCDNRITMS
                     YPKVRKEIAKGLASKIKEAYPEVQVIAGTATAGIPHAAWVAEILDLPMVYIRSKAKDH
                     GKGNQIEGRIFEGQKMVVIEDLISTGGSVLEAAEAAKREGADILGVAAIFTYELPKGK
                     ANFEKAEIPLMTLTNYSVLIEAALEDRYIDEQELTLLKEWKKDPENWQI"
     gene            47980..48294
                     /locus_tag="IXT20_000293"
     CDS             47980..48294
                     /locus_tag="IXT20_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4858770.1"
                     /translation="MADLSALDEDREIRFVSPESSIGIAKIQKLIARSIFSSGMKRNF
                     YRDQFLIFLKFIFLRLVSRKKRIISSLFSIIYRFSLFFGKGAVSKMVKSGNVANISYL
                     MI"
     gene            48332..48973
                     /locus_tag="IXT20_000294"
     CDS             48332..48973
                     /locus_tag="IXT20_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAQ4858771.1"
                     /translation="MGLNEFILGLNNLETITRAPGFFKYTEHTVAAHSYRVASIAQVL
                     GDIEEANGQPIDWKLLYEKSLNHDYTERFIGDIKTPVKYASKELRGMLQTVEEKMTDE
                     FINQEIPAEFQEVYRRRLFEGKDETVEGELLAISDKVDLLYESFDEIIKNNPEKVYKE
                     MFIEAVTTIKSFEHRPSVQYFFKEIFPELLNQEFYGKEAFLETVSTIVKKEEK"
     gene            49004..49708
                     /locus_tag="IXT20_000295"
     CDS             49004..49708
                     /locus_tag="IXT20_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase family protein"
                     /protein_id="HAQ4858772.1"
                     /translation="MKKNMDVEWGYSGDKGPENWASLCDWFARGAEFPLQSPIHLTKG
                     EETKIEGDTLSFHYQKQRFTDKEFKNTIHLVPFDQLSYVEFKKERYYLTDIHFHLPSE
                     HIINGKQEDIEFHFVHMNSKKENLVVGVMYQLMVQEGWLYNQENGESWNLEKHEHWVN
                     PDVFFPEQKSHFHYIGSLTTPPTSGPIQWFVMDHVGKLNQEFLLPFDGQYARPNNRPI
                     QPLNGREIYYFEEFEQ"
     gene            49705..50607
                     /locus_tag="IXT20_000296"
     CDS             49705..50607
                     /locus_tag="IXT20_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAQ4858773.1"
                     /translation="MNIQQMKYVVAVANNGSFREAAKKMFVSQPSLSAGIKELETELG
                     ITLFTRTNRGAYLTEEGQDFLNRAEKILVQLDTLETHYLTSEKRDNFSIAAQHYDFLG
                     PLTAQLLEEFKDEIKQFRIMETTTARVIEEVKEQHSEIGIIYMNEHNQAGIRRYLQQG
                     ELSGRSIGKFKTHIFLRQGHPLAEHAEITKEELLSYPQVRFTQDGNNFPYFYEDLIEI
                     PDQETVIYTSDRGTLMNIVLETDAYASGSGIVIGGIREHLRLIPLAEGELNEFYIIYS
                     AKRPLSEIAQRFIKELTRLLDQQS"
     gene            complement(50765..52471)
                     /locus_tag="IXT20_000297"
     CDS             complement(50765..52471)
                     /locus_tag="IXT20_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin/fibrinogen-binding protein"
                     /protein_id="HAQ4858774.1"
                     /translation="MSFDGVFTHAMVNELRETLLSGRISKIHQPYENEIVLVIRSRGK
                     NHRLLLSAHPSYARVQITQIDYQNPDNPPNFVMMLRKYLDGAILEDIEQIENDRVIHF
                     HFAKRNELGDLQNIILIVELMGRHSTIVLVNRETGKILDAIKHIGSSQNTYRSLLPGV
                     EYVAPPKQEVLNPFSSEKEKIFQRLSQTELDPKAIQRQFQGIGFDIAQELTKRLLERP
                     NEKMVVWDEFFSAISHQPIPTFYETENKDFFTPIAYQVLSEQASAVTAYPTLSQLLDS
                     YYHEKAEKDRAKQQGGELIRKIENELKRNKNKLKKREQTLKESENAENYRRNGELLTT
                     FLTQVPRGAKEVVLPNYYEEDRPIKIALDPALTPNQNAQKYFHRYQKLKNAVKLIGEQ
                     IQEAKDEIQYLESVLSQLEIAGPMDIEAIKEELTSEGYLKKKNLKKQKRKKPSQPDQY
                     FSSDGTLILVGKNNLQNDQLTMKTAKKTDYWLHAKNIPGSHVIIKSDKPSDETITEAA
                     ELAAYFSKYRYSAQVPVDLVQVKHIRKPNGAKPGYVIYENQKTIIVTPEEEKITAMKQ
                     NR"
     gene            52661..53284
                     /locus_tag="IXT20_000298"
     CDS             52661..53284
                     /locus_tag="IXT20_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4858775.1"
                     /translation="MFVIWEVVKSRVQEMNEHFSVSFAENPSKYFRRLVFLLILSLLL
                     TVAMFLLPEKIREEQVNSLVYSSIPTSKLKPFDYSKADQKIKQSRAISVMFSKPNGKT
                     YQDMLDVFNDPELMEELNRPIFYYPIVYDVHELEQKYNIRTDEVTFIFFDGGKEANRI
                     TAGKGGITDFDKELIPELNRLPLANIKQLEEELDRTGVTSQNSEIAN"
     gene            53734..54783
                     /locus_tag="IXT20_000299"
     CDS             53734..54783
                     /locus_tag="IXT20_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAQ4858776.1"
                     /translation="MKNKRLITVVVIILVYLAGAFIYGKASGEKESTSTNQTKKTTVV
                     GVLQYVSHPALDEIYRGIKDGLKEEGLKEGENLEIKFQNGQADQSKLATMSQQLVQSD
                     PDALIGIATPAAQSLANTTSSIPVVLGAVTDPVGAGLVASLEKPGGNVTGVSDQPPVT
                     SQIKLASDLLPNAKKVGILYASSEDNSKYQVAQAEEAAKKEGLESVKYAVPSTNEIAQ
                     TVQVMSRQVDFIYVPLDNTIANAMQTVVKEANKANIPVIPSVDTMVEQGGLATIGINQ
                     YQLGLQSGKMAAELASGKEKPETTPVYTFDQGDTVINQSQADRLGIIVPQSMKEKAKI
                     ITDDSQQETSKEDDK"
     gene            54780..55661
                     /locus_tag="IXT20_000300"
     CDS             54780..55661
                     /locus_tag="IXT20_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ4858777.1"
                     /translation="MIVSAISQGMLWAILGLGIFMTYRILDFPDMTTEGSFPLGAAVC
                     VTAITHGVAPIVATLLGVGAGMIAGLVTGLLYTKGKIPVILAGILVMSGLNSVILFVM
                     RTPNLSLLNHPILQDSFHRFGLPDYFDTVLLGFISLGILIAVLLFFFNTDLGQGYIAT
                     GDNETMARSLGIKTDRMKILGLTLSNGVIALSGALIAQNDGYADVNKGIGVIVIGLAS
                     IIIGEVIFQELTLAERLIAIVVGSIIYQLLILVVIKLGFDTTYLKLFSAIILAICLMI
                     PQLKKALNLKTGFEKEA"
     gene            55664..56434
                     /locus_tag="IXT20_000301"
     CDS             55664..56434
                     /locus_tag="IXT20_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ4858778.1"
                     /translation="MTDILTIKNGRKVVNNGMNEEKVLLDEINLTIREGDFITVLGGN
                     GAGKSTLFNTIAGTLQLSQGSISFKDQLITKDSEEKRARFLSRVFQDPKMGTAPRMTV
                     AENLLLAQKRGQKRKFRLRNLKNQKEDFFRLCQEVGNGLEQHLDTPAGELSGGQRQAL
                     SLLMATITAPELLLLDEHTAALDPKTAKALMRLTNQRINEQKLTCLMITHRMEDALKY
                     GNRLIVLQKGKIVQDISEKEKQHLTMGDLLQFFEEVVD"
     gene            56713..57567
                     /gene="aroE"
                     /locus_tag="IXT20_000302"
     CDS             56713..57567
                     /gene="aroE"
                     /locus_tag="IXT20_000302"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HAQ4858779.1"
                     /translation="MISGQTKLAGLFADPASHSLSPLMHNTAFEANNIDAVYLAFQVN
                     PMNLKQAVESIRTFDMLGVNLSMPNKTAVIPYLDEVSQEAQLIGAVNTIVHEKGRLIG
                     YNTDGAGFMQSVQEAGISIKKKKMTLLGAGGAAKAVTVQAALDGAEEIVVYKRNNQTF
                     AQVKETFEQLSYRTSCKIKVRDFADTHQLRKDLKESCLLVNATDIGMGKKINQSPLLD
                     ISMMHENLAVFDLIYAPRETRLLKEAKKVGAKGYNGLGMLIYQGAIAFELWTKEKMPI
                     HVIEKLFD"
     gene            57745..58908
                     /locus_tag="IXT20_000303"
     CDS             57745..58908
                     /locus_tag="IXT20_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HAQ4858780.1"
                     /translation="MSRMDRYKDIHKKAKPLKRDTGSIFARRERKRTRMENEEDATRI
                     YEREQHSFEHLNQYEQQIYRDAPRAEEQQKNKKFFNRQKKNKPLKPRRKRSWKKIILS
                     ILLVLILYSGISFFIGKYVAEHDTSLEAATSETFHGQKATNGAHNILILGSDTRGEDA
                     GRADTIMVLQLDGPSHKPKLISFMRDSFVTIPGVGQNKINSAYAYGGADLVRQTLVEN
                     FGIDCQYYAKVDFKSFEKVIDALFMNGVKIDAEKDLNLDGVDIKKGGQKMDGHVLLQY
                     ARFRMDEQGDFGRVRRQQQVMNAIFSQLKNPLNLIRAPYAAGKALGYTSTDVTAWFLT
                     KNLFSIARGAGGIDRISVPVDGSWNYGQSDYAGSILEIDNQMNQSAITDFLNK"
     gene            59025..59435
                     /locus_tag="IXT20_000304"
     CDS             59025..59435
                     /locus_tag="IXT20_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HAQ4858781.1"
                     /translation="MDIYLNFKNESREAIAFYEKVFQTEASGITTYGEMPGIEQMNLP
                     EEAKNLITNARLNIYDTLVMFADTPEGIGPELVVGNNVSLVIQTRNEEETKRLFESLA
                     EGGKIEMPLDAVPWAQLYGMVTDRFGINWQINQE"
     gene            59609..61837
                     /gene="topB"
                     /locus_tag="IXT20_000305"
     CDS             59609..61837
                     /gene="topB"
                     /locus_tag="IXT20_000305"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HAQ4858782.1"
                     /translation="MKTVILAEKPSQAKAYAETFQQQKRHEGYYEISDPLFAGEVTIT
                     YGFGHLVDMVAPGAYEERWAKWSLENLPIFPETFRYEVPMDKRAQFSIVKRELQSADT
                     IIIATDGDREGEAIAWSIIRQANAFSKEKNYLRLWINSLEKEAIYEGFKNLQPGKNYF
                     PKYKEAQARQNADWLIGMNGSPLYSLLLQRKGIDGSFSLGRVQTPTLYMIYQLQEEIK
                     HFKKETYFEGEGLITTNKGQFSGKIVPKKAFKTQEELTKEIEKLGAHLGKQNGRILEV
                     TKKEKRLHSPRLFSLSSLQTKMNQLMKASAKATLDAAQGLYESKFLSYPRTDSFYITE
                     NEHQYLVKHLQNYKKFLGIEEVQTTEIKAKKRYVDAKKVQEHHAIIPTKTIPTPARFA
                     KLSKLQQAIYLQVLRTTVAMFAADYIYEETTVMTGVQQLKLKSIGKIPLQQGWQEILR
                     IKSKKKEAQTLPPIVENEEAEVELKSMEKETQPPKPYTEGTLITAMKTAGKTLDNDEA
                     QEILKEVEGIGTEATRANIIENLKQKHYIEVNKNEITVTPKGITLCKAVANEPLLTSA
                     EMTARWEGYLRKIGKQEGTPSVFLENIKKFILHLLAEVPNQIDKVDFSDEISGTRQIK
                     AMEKKNDQLGRCPKCKKGIVMLYPKIATCLNPECDFKLWPTVAKKKLTKTNLRDLLSK
                     GKTSKVVKGFTGKKGKFDAVLVLKEDMTIGFSFPWIENSGTKAVDGNKETSRKNNENE
                     VE"
     gene            61975..62292
                     /locus_tag="IXT20_000306"
     CDS             61975..62292
                     /locus_tag="IXT20_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4858783.1"
                     /translation="MKKVHSFLNYLMGAITIVAVYQAFLKYPDYGTKLLSLTHNQTAY
                     SPTLILSTLTAGAKSATYFFHKTTSMLKRKKQRKQLLVVVAAMIIIYFLPVITQVFHQ
                     YFG"
     gene            62452..62688
                     /locus_tag="IXT20_000307"
     CDS             62452..62688
                     /locus_tag="IXT20_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAQ4858784.1"
                     /translation="MKKMKKYKIRKSGNSDVTTIPPEVKEFMGVQTGDAISYVFQSDG
                     SVRMIKAQEEPDIDSLVDSIMNQYEDALKDLVDL"
     gene            62685..63083
                     /locus_tag="IXT20_000308"
     CDS             62685..63083
                     /locus_tag="IXT20_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HAQ4858785.1"
                     /translation="MRYLSTKEIIKINAKVILRHSPGEMIGVKDANALDMAVKQPSQA
                     VFNQELYPEIYEKAAILAINLAKKHPFHNGNKRTALVAMLLFLQLNNCPVAFSRQEAV
                     DFIIMITTSSLDFDSLKSKITSYLRQTAIK"
     gene            complement(63750..64694)
                     /locus_tag="IXT20_000309"
     CDS             complement(63750..64694)
                     /locus_tag="IXT20_000309"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HAQ4858786.1"
                     /translation="MKKTSRKVVIVGTGFVGTSIAYAMINQGISNELVLIDVNQEKAE
                     GEALDLLDGMAWGDENVAVWSGGYEECKDADIVVITAGINQKPGQSRLDLVKTNASIM
                     RQIVKEIMGSGFDGIIVVASNPVDILTYIAWNESGLPTSRVIGTGTTLDTTRFRKEIA
                     LKLKVDPRSVHGYILGEHGDSEVAAWSHTTVGGKPVFEIVEKDHRIAQDELDVIADKV
                     RNAAYEIIDRKKATYYGIGMSTARIVKAILNNEQAVLPVSAYLTGEYDEKDIFTGVPS
                     IVDENGVREVVELSINEEEKAMFSKSTSALREVLNTVL"
     gene            complement(64990..65934)
                     /locus_tag="IXT20_000310"
     CDS             complement(64990..65934)
                     /locus_tag="IXT20_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ4858787.1"
                     /translation="MRNEESIETLQREQGMNINDLNLAYHLQTPPKIHRGDLFQQGNI
                     HISKHRRYAEVPEHTHDFVEINYMYSGTCLQIINGERIEMEESDVVIIDKEATQRIEY
                     TGQEDILINILLKEETLSTEILNHLASTNNLLSSFLINASKEKGEHTQFIHFRGGKTS
                     RLTHLIENMLLIYFDTYSHKIRSLNLYTSLLLIELTHLLEQQSFSSADGCAENEMLRV
                     LDYIDKHFTSCTLKEPAAHFGYNPVYLSNKLKMYFDASFQELVLKKKFDFALELIQET
                     SFSLEEIANRIGYKQTTSLFKLFKKYSTCTPNEWRNKH"
     gene            66155..67369
                     /locus_tag="IXT20_000311"
     CDS             66155..67369
                     /locus_tag="IXT20_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ4858788.1"
                     /translation="MLMNKKMMKFSLLSVSLILTSAGAVTSAIPSMSATYATRSLSAV
                     ELLTTAPSFMVTIFVLISSFIARKIGQKQTVIIGLVIASICGILPLFSQSYSIVLASR
                     AGLGIGFGLINSLAVSMIAAFYHGDERASMIGFQSAFQGFGAALMTFVAGQLVQFGWQ
                     YTFLVYAISLPILVLFVLFVPTPETTELQSHGTAPTRQVIKTNPKMISYGIFLLLVIV
                     VYNAVSIKLATVLTTYSLGNETNAANILTIMQLASMAAGVCFGWLQTKAHRYMLTLSL
                     LLMGIGFVLIATVINLYVIGFGAILTGISFSLFIPYLFNQVSIQAPKYAESFNTSILL
                     VGANLGTFIAPYGLLFLSFASLGSKTLAVFVNGSILLLISALISLLVVHRTNRKTVIR
                     VDEEIKEQLTTK"
     gene            67391..69406
                     /locus_tag="IXT20_000312"
     CDS             67391..69406
                     /locus_tag="IXT20_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 78 glycoside hydrolase catalytic domain"
                     /protein_id="HAQ4858789.1"
                     /translation="MTNEKHKDRWLWYPGDFEIRHGLLQNFQREERGFDWPAYWYMDD
                     CHRNVKFKRYYFLDQPSMFKVTIQGVGYVEINGQKHPCGKWLTCPAGKAKIRIFVGHT
                     SGLPAMFIEGDEVKSDIGWTASNFIEEYPAGWSPLYVDIAKDPNQIYYQKECVHPVTE
                     TEIGGGILFDFGRAVNGCVELISLDDHIESITLCYGESDVEAMDIDYCYYKQIGMTRK
                     MKARKRAFRYLFIPEVSTGMVQLQVFHEFLSLSKQAAFSSDSSLLNQIWSVSEEAFKL
                     CSDLFFIDGIKRDRWIWSGDAYQSFMINQYLFFDEEINKRTILALRGQNEIKQHMNTI
                     VDYSILWLIGIENHYMMTKDYEFLRIVYPKMQSLMRYLMEQTNELGFIYGRAKDWIFI
                     DWSEMDKEGTLAAEQVLLLKAYHSMITCGDILAETYGENENYHTHLYQEKYQLLKQNL
                     FDYFWDEELGAFIDCYESGRRNVTRHANIFAVLFDFANKDQQQSILKNVLLNDTITQI
                     TTPYFKFYEQDALCKLGQTNIVYQTILDYWGGMLSHDAVTFWEEYDPTQTGNEKYSMY
                     GDPYGKSLCHAWGASPIYLLGRYFIGLYPTKPGYEEFMIQPNLDSFETLNCTLPIKDG
                     KISLEKTKDKIVITSDRTGGTVKMNGKSYPLLAKQPLVLTSYSKINA"
     gene            complement(69489..70862)
                     /locus_tag="IXT20_000313"
     CDS             complement(69489..70862)
                     /locus_tag="IXT20_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HAQ4858790.1"
                     /translation="MDKLLNLFVSIVSQPSILVSLIALLGLVLQKKKFSEVIQGTIKT
                     FVGFLVLIGGAGLISNSLTPFASMFQTALHTQGVVPSNEAVVAIALQQYGTPTALIML
                     VGMIVNILLARFTRYKFIFLTGQAMLYVSCLTAVILVSAGFTANLWMILLGGVFEGTL
                     LTITPALCQPFMKNITGNNQVALGHTGNIGYASSGWIGKFFGNKDRSTEDIKIPKSFS
                     FLRDSTVSIMLLMSIVYVALALLAGPGFVERELSNGDNAVIFALIQAGTFTAGFVIVL
                     QGVRMVLAEIVPAFQGIAKKLVPDSKPALDVPIVFPYAPNAVLIGFFVSFFVGTLSMF
                     AMIGLQTTVIIPGVVGHFFCGAASGVFGNSTGGRRGAVLGSAFNSLLISWLPLFILPV
                     LGDLKIASSTFADTDYLVPGLVLGKLGEYGQIALTVGIIAFFLLVVLSSFLFAGKRTN
                     IVSKEQN"
     gene            complement(70888..71172)
                     /locus_tag="IXT20_000314"
     CDS             complement(70888..71172)
                     /locus_tag="IXT20_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ4858791.1"
                     /translation="MKIFAVCQSGLGTSFMVEMNIKQALADLGADVDEFEVSHTDVGS
                     VSSDMADYFFIEHSLKDTLTSIPDEKLVPLQSIIDSEEVKEKVTKILSKE"
     gene            complement(71174..73189)
                     /locus_tag="IXT20_000315"
     CDS             complement(71174..73189)
                     /locus_tag="IXT20_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HAQ4858792.1"
                     /translation="MNYKISYLLKTPREISLEKAAEIIEVNTSEAIKQLTPFAEYVDD
                     TRIRIKKFRAIDWAEKFLDITRFEAVYTEQERRDLLYLMVYSTATSVSVFHFQDFLQV
                     SKGTVLSDIKKIRNELEVEDCQLSYTRKTGFRLLGSEAILRREAKNRTARLMQSLAGR
                     FGLTYLTATLSIDYYAKVRDLLDKTISLSDLRFAPNRMDELAYYLSLTTIRIGKTSME
                     LVKDAELLASLAVTDISRQVLTGLGYSEHQNSEIIFFSLCLASIVEGDVQEPALDFLL
                     ECSSEVIHRMEFLMAVEFASFRELLMNVFYHLVPAYFRISYSFYLPNVMIDQIKHQYA
                     SIYEMTRKALLPLERRIGKSIPEEEIGFFTILFGGEIRKADAEERNRKIRAVIVCPSG
                     ISSSLILKSELQQLFPMIFFTETNSADRLHEVDETSYDIIFSTVPLTLTDKKKSLYVL
                     HPIMTDLEKNQLINQVQRDWLIPGFSMPSAQELLSALLPYIELKEGVDEEKLYQVLNR
                     KMNKLLEKKEDQRPMLSELLTEEMIQLSDQPKNWQEAITFAAQPLLSENKIEESYIKA
                     MIERVEQYGAFIHIGDHIALPHARPEDGVNEVGMSLLKLQQPVDLLDNPAHPISLFIC
                     LAAIDNEAHLRALANLTKLLSNKQNLQDLLHASTKEEILAIIRKGDE"
     gene            complement(73368..73673)
                     /locus_tag="IXT20_000316"
     CDS             complement(73368..73673)
                     /locus_tag="IXT20_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA processing protein"
                     /protein_id="HAQ4858793.1"
                     /translation="MTNYDGEKHKRLRGSMAPKTEEEVALAGTNSGETEDKEESLAGT
                     NSGETENKETALSGSNSVEEETKPNEPNHFEKDIEKAVEDVKEKFEELTSKNKHDHH"
     gene            complement(73762..74793)
                     /locus_tag="IXT20_000317"
     CDS             complement(73762..74793)
                     /locus_tag="IXT20_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydrogenase"
                     /protein_id="HAQ4858794.1"
                     /translation="MLTIAYLGNGKSTNRYHLPFSRKLTNKINIKKIFSPSEPVWKTF
                     EDIEYTDQLSDLWDDPEIQLVVITTPSQFHYEYGKMALEHGKNVLVEKPFAETSKEAE
                     ELFALAKEKGLFIQCYQNRRFDSDFLTVQKVIESGKLGDILEVEMHYDYFRPEVPENT
                     HEFSTANSYLYGHACHTVDQVLSYFGEPDDISYDVRQLLGKGRMNDYFDLDFFYGNMK
                     ISIKSSYFRIKERPSFVVYGKKGMFVKQTKDRQEEHLKMFYMPDHEDFGIDLPEHYGV
                     LTYIDEQGSYHEEKVISEVGDYSRVYEGIYETLVNGAEKVIKDEETILQMKILEEGIK
                     SIQESEGKI"
     gene            75000..75875
                     /locus_tag="IXT20_000318"
     CDS             75000..75875
                     /locus_tag="IXT20_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAQ4858795.1"
                     /translation="MLIEEKLTKQELFTTTEKRIVDYIRRNIEAAVYMTIEELAKATY
                     TSHSAIIRLCKKMGFSGFKEFRFELGREVHQLIAQFNQTIDPNFPFSSEDSTQQIAKK
                     MADLSIQSIKKAQLQIEHEDLDQIGKVLNKAQRIFLFAKGDSQITARKFQNKMVKLNK
                     FLIMAEEYSDSSWNAANLTSEDCAIFISYSGRIHHYERIMTYLKHVEAPTLLITGNQH
                     SEMAKQADMCLVISQEEYDFAKVATFSSQIAFDYVLNTLFSVIYSQNFEENIRNLKNK
                     QELMQKGLLKEYRKT"
     gene            complement(76027..76869)
                     /locus_tag="IXT20_000319"
     CDS             complement(76027..76869)
                     /locus_tag="IXT20_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAQ4858796.1"
                     /translation="MKPKKRKLLDLLMLFLLLSGIGILAYPFVSDALNNYLDQQIISH
                     YQQQAVKENEEVMAKIQENMTKKNQQLAKEGGNPGADPFTKKKEPAKKDRTYFEKHTI
                     GILTIPEINVHLPIFDETNTVLLEKGASLLEGTSYPTGGENTHAVISSHRGLPQAKLF
                     TDLPKLTLGDSFYIEINGRTLAYQVDQIKTVEPTDTKDLRIEKGQDFVTLLTCTPYMV
                     NSHRLLVRGHRVPYHAKEVQKEVQKVSENKRFFLYGLLAACILIFLLLIYLFRQSFKI
                     RNRS"
     gene            complement(76961..78838)
                     /locus_tag="IXT20_000320"
     CDS             complement(76961..78838)
                     /locus_tag="IXT20_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HAQ4858797.1"
                     /translation="MKNHKKINVMLGVLFLILPLLTNSFGAKKVFAEETAAQVILHKK
                     KMTDLPDPLIQNSGKEMSEFDQYQGLADISFSVYNVTQEFYAQRDKGASVDAAKQAVQ
                     SLTPGTPVASGTTDADGNVTLSLPKKQNGKDAVYTIKEEPKDGVSAAANMVLAFPVYE
                     MIKQADGSYKYGTEELDTIHLYPKNTVGNDGTLKVTKIGTAENEALNGAEFIISKEEG
                     TPSVKKYIQSVTDGLYTWTTDQTKAKHFITGHSYDIGNNDFAEASIEKGQLIVNHLEV
                     GKYNLEEVKAPDNAEMIEKQTITPFEILANSQTPVEKTIKNDTSKVDKTTPQLNGKDV
                     AIGEKIQYEISVNIPLGIADKEGTQNKYTTFKLIDTHDAALTFDNDSSGTYAYALYDG
                     NKEIDPVNYSVTEQTDGFTVSVDPNYIPSLTPGGTLKFVYYMHLNEKADPTKGFSNQA
                     NVDNGHTNDQTPPSVDVVTGGKRFVKVDGDVTSDQTLAGAEFVVRDQDSDTAKYLSID
                     PSTKAVSWVSAKESATVFTTTSNGLIDVTGLKYGTYYLEETKAPEKYVPLTNRVAFTI
                     DEQSYVTAGQLISPEKIPNKHKGTLPSTGGKGIYVYIGAGVVLLLIAGLYFARRKHSQ
                     I"
     gene            complement(78835..80256)
                     /locus_tag="IXT20_000321"
     CDS             complement(78835..80256)
                     /locus_tag="IXT20_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HAQ4858798.1"
                     /translation="MKKLGWLSMCLFLLLFKPAFTQVATETETEMVQITLHKLLFPNG
                     QLPKNHPNDGQEKALLQTYRGLNGVTFQVYDVTDSFYHLREKGKTVEEAQAEIAKNGA
                     SSGMFTAEATTTTLNNEDGIASFSLAAKDQEKRDKAYLFIESKVPEVVKEKAENMVVV
                     LPVHGQNNQKLSTIHLYPKNEENDYPDPPFEKVLEEPRNDFTIGEKITYSLHTTIPVN
                     ILDYQKFELSDSADEALTFLPNSLTISSNGEKLTEGFVIHKKPHGFDVLFSIPSLEKY
                     AGKKLTISYQMQLSSTAQANKEINNNGTLDFGFGVSTKKVSVYTGSKQFVKIETNKPD
                     KRLAGAVFLIKNKAGNYLQQTANGYKWTKNESDALHLISDKNGAFSISGLKTGSYRLK
                     EIEAPSGYILSETEIPFTISTFLSEDKEADSILKVVNKKENSRPFLPKTNETKNTLLG
                     VVGMVFASFAIWLFIKKRTGVKK"
     gene            complement(80259..83648)
                     /locus_tag="IXT20_000322"
     CDS             complement(80259..83648)
                     /locus_tag="IXT20_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HAQ4858799.1"
                     /translation="MTTTGKKLKVIFMLIILSLSNFVPLSAIADTTDDPTVLETISAE
                     VISDQSGKKALNIKLNANNTSAEKIEKEIGLVENYLSDVERKEGDGYAYQVNSGKITL
                     EISSNTKQTIDLSFPIDPALYHSQANKLIVDNKEYDIIDETENKKDTDVSVPKPDEIE
                     EESSKENENSVSPFTLPTLSLPAVSVPSNQTIPTEYTTDDQGTYPKASWQPTGNTNVL
                     DHQGNKNGTNQWDGINSWNGDPNDRTHSYIEYGGTGNQADYAIRKYAKETSTPGLFDV
                     YLNARGNVQKDITPLDLVLVVDWSGSMNDNNRIGEVKIGVDRFVDTLADSGITDKINM
                     GYVGYSSEGYSYSNGAVQMGSFDSVKNQVKSITPSRTNGGTFTQKALRDAGSMLSVPN
                     GHKKVIVLLTDGVPTFSYKVQRVHAQSSSNYYGTQFSNTQDRPGNTSLISRIYDAPDQ
                     NNLSRRIDSTFIATIGEAMALKERGIEIHGLGIQLQSDPAAGLSKAEVESRMRQMVSS
                     DEKGDLYYESADHATDISEYLAKKAVQISATVSNGQINDPIAEPFIYQPGTLSVKSVG
                     TSPTTVTPSISIEGNTIKSNQIYLGKDQEIQIHYQVRIQTENEDFHPNFWYQMNGRTT
                     FQPNIDTNELAEFGIPSAKAPGVSLHIKKLWEEFDNNLADRPDQVTFEIQREHTTNAA
                     AWKNGYIRIIKPAKDTTNTWERADIDKLSANSGESYQEILSLPQYNNQGQAFSYQTIK
                     ELPVPGYDSQQIDAMTWKNTKQFTPLNLKITKNSSTGEKDLIGAVFKLTGDSIDTLLT
                     DHGDGTYSLPENVKLQKEMTYTLTETKAPEGHGLSKKTTWEIKIASDGTVTIDGKTVT
                     TSDDTIQLTIENPFVEVPVAVRKYAMQGTDKEINLKGAAFSLQKKEANGTYQPIDSQT
                     TNEKGLASFDSLTPGKYRVVETAGPAGYDTSPGNYEFQIDKYGKIIYTGKNTEMTNNV
                     WTLTHQNRLKAFDLTVHKKEDNGQTLKGAKFRLQGPEMDLESPKDGQETDTFLFENLK
                     PGTYTLTETFTPEGYQGLKEPVTIVIHEDGSIQVDGQDHESVLSPGAKNNQISLDITN
                     QAKVPLPETGGIGRLGIYLVGMIGCAFSIWYLFLKKERGGS"
     gene            83905..85296
                     /locus_tag="IXT20_000323"
     CDS             83905..85296
                     /locus_tag="IXT20_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAQ4858800.1"
                     /translation="MEKIIRILSISDKWMTKKELAKRIGSSESTFVRYIEEIKIRWGT
                     DVTIQSSQKLGYRLEHLNVPAYLNVLMEMSQSSTNSRLLNEIIVNPGKTIEYYCEKIS
                     ISRSSFTRKLKQCNQILESYLLKVIVDQGFQLTSLKSELQLRIFVTFFFLSYYGRRTL
                     PFPIEKKKIKEIMVKNNCQMNLFQEESPYEQSFFIMYFVVHLMRESQGTPFEWKAGDS
                     YVQLINIDKKDFLILKNLLGKLKYSSYIKVVNYFVNTIFSEFSEKEKNEISEQIRKNM
                     SYCSFSSADCKEKNEEYVVQLLTQIYFFSRIFPYDTTHLTIRATYFAQQMQHRQIRLY
                     AGFRAELQFYSTLLNQNLIYYLSSYIFWMLIGNPEIHHYTSDKKILIISDLSLRHSQY
                     IEEYISDILAVHKIHSETTAITEDQLSKYNLLEYDLIVTNQPILNAHVPHILIDDSVS
                     FANEEELIRLFEL"
     gene            complement(85369..86016)
                     /locus_tag="IXT20_000324"
     CDS             complement(85369..86016)
                     /locus_tag="IXT20_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAQ4858801.1"
                     /translation="MTMKGYPRIIRDKGEMWMPKETFFNLSNAKKQRIDDLLLEIFCS
                     QHISQVKVSEIVDKMGMSRGAFYKYFQDLEDAYLYTIKSSSHIVHQDILKFISENKQH
                     FFLGIEKYLEWCCDLDHESIYWQRLYLLTQRNDQQTTKRRELSADSPMNEKWLELLRI
                     NHFSIQSSEEAVSFLYFSMTLVMNTLTDLIANNWTKEELLKDFRYKSRWIAEGIQ"
     gene            complement(86024..86710)
                     /locus_tag="IXT20_000325"
     CDS             complement(86024..86710)
                     /locus_tag="IXT20_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002366355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ4858802.1"
                     /translation="MKKILTMKKVTKTFGSGHTKVSALKAIDFYVKEGEFVSIIGPSG
                     SGKSTFLTISGGLQTPTSGEIVINGHSLTELPEKKRADLRFKEIGFILQSSNLIPFLT
                     VKEQFELVDQVTSKKEDSTKIDSLLQSLDIQSLKNSYPKDLSGGERQRVAIARALYNE
                     PSLILADEPTASLDTDHAYEVVKLLVKEAHEKQKATIMVTHDVRMIQWSDRVYRMEDG
                     YLTEEEKKKE"
     gene            complement(86716..87789)
                     /locus_tag="IXT20_000326"
     CDS             complement(86716..87789)
                     /locus_tag="IXT20_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HAQ4858803.1"
                     /translation="MFLAIKEIRYSKLRYGLIVGIMFLIAYVVFMLSGLANGLSEEFK
                     KAIDDWDAQEIVLSEDANQVFAASQLTRGDLKYIEGGEKAPIGLYSGAIKGKNKENVT
                     VFGTTKEAFLLPKLTKGHEFKTKNEIIISQNLADSGYKIGDTIKIGSYDDPLTIVGIF
                     PETYYTVSPVIYASLDTWTALKYGQQPFASEEEQPINGIVIKDSAKISKEKASKGLQL
                     LTIGTFIESIPGYSAQNMTLNAMIYFLFLVVAAVVGIFMYVMTLQKTAIFGVMKAQGI
                     KNFFIAKSLVAQSFIVGVVGVLLALLFAYLTSLILPSAMPFAVFWSQWLLYSGILIIV
                     AMLGGLFSIRTITKVDPITAIGG"
     gene            88011..88685
                     /locus_tag="IXT20_000327"
     CDS             88011..88685
                     /locus_tag="IXT20_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ4858804.1"
                     /translation="MIKLLVVEDDKVLSNNIKEILAAIGEITQVYDGSEGLFELESGV
                     YDLAILDLMMPEMNGYEVLAKIRKEKIQTPVLILTAKDSLEDKIEGFERGADDYLTKP
                     FYREELIMRVKALLKRSLGLFDENVLKYKDISCDLKSNEVLYKEQVLPIQGKEFELLV
                     YFLQNKGIILTKEQIFDRIWGFDSDTTITVIEVYMSNLRKHLRSTGLDKEIKTLRNVG
                     YILQRV"
     gene            88690..90030
                     /locus_tag="IXT20_000328"
     CDS             88690..90030
                     /locus_tag="IXT20_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAQ4858805.1"
                     /translation="MNGDITRKQRSRFFATNVAAFAFIFFLLGFITLQILNQSAYRET
                     DLSLEKINFDSRTVQLEIQRYQQGDPFLNPTQTEQNRTNMEQPLMEPSNDRFNTQVIL
                     WSKNGEILNKAAIGGRFNQIKELKMDTKNLNTIQNIELNEDSGSSALSFRSITKKAET
                     NQDGVAYIQILENTNQIKNSLATFRTIMILCMIVFWILSIGISYYLSSLSMRPIILSW
                     RRQKEFVENASHELRTPLTIIQNSLEHLFTKPNHTIIEESESIAQALSETRRLTGLTS
                     DLLTIARNDSDQQLLSKQMINTQEYIEQLVKPFQEMAIIDGKEFILHNDATLEVPIDE
                     KKIHQVLVILLDNALKYTNPEDKIILDSSVNHKEWLIHVKNTGPSISEEDQARIFDRF
                     YREDQSRAKETGGYGLGLAIAKQVIEQHHGQISVSNYLPQGVDFKVQLPLKPKK"
     gene            complement(90179..91165)
                     /locus_tag="IXT20_000329"
     CDS             complement(90179..91165)
                     /locus_tag="IXT20_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2382 domain-containing protein"
                     /protein_id="HAQ4858806.1"
                     /translation="MEEKNLTVIGSYSTREEALSVIERLRNEGYERDDIVIYTTDEAA
                     SRLGFDGLSGIDVETDENRMDGEEDRSLWEKIKDTFSFDTYDSETATPENDPLYQYRG
                     DISDGKFVITVKGYRQPETTEDTMDTQDTVSPASTMGTGLETGTGTDPMDVGGTTGFQ
                     NTTDPLNMTPGTDPMDVPGETDTMGTSKTEDIDRKPNLDDDTIQLKEEKLDVNTHDVT
                     TGEVDIHKHVVNDTETVEVPVKREEIVIERKPVTDQSSQGTDEKLEDDTITIPIKEEQ
                     VDVSKHTVIREEVGIHKEEHEDVEKVTEDVSREELDIDTSGDVHIEDRNKKS"
     gene            91889..92920
                     /locus_tag="IXT20_000330"
     CDS             91889..92920
                     /locus_tag="IXT20_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAQ4858807.1"
                     /translation="MDSKTRKQLQEKLKQRTHLLPSVFQQFLEENRSSLSLGSRYEYA
                     KDFHLFTDYLRKVFSEEVDDQLIIHLEKQDYQNFLAKIYRHTRSFQTTANQETEQLFE
                     NSYYGISRKQYSLNHFLRFLYQKGLIEEEISLLGTSLPETTKAAPRYIDAELFKDFEH
                     LFIPIEGFQTSREASFEEKNRPRNLAITAILLYCGLKIHEVVELKRKDVSLTKQILNL
                     NRKENRLNKVPIPKEAMPFLENYFKLTAQNTDENSFVFRSSHDQQISPRTIRQILSKI
                     TVYKNVSPELCRKTYRYYAELYLDDADAVNYLCGNRYLSSHSFQDIWEKMVDFSYHEL
                     AAFVRVSSI"
     gene            93029..>93453
                     /locus_tag="IXT20_000331"
     CDS             93029..>93453
                     /locus_tag="IXT20_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HAQ4858808.1"
                     /translation="MMETITYSPLNLFTNFQEAAANFSETPIIFDEPLSAFPELGLET
                     TYKKCCESVLKRAYQLAHLGVKSGDKVIIYKSSAFDTYLLAVSVSYLGAVPVMTSYHL
                     PTTTMEVFIDRLEDPFILFDDETKKRVREISNGTKSKQIP"
BASE COUNT        29895 a        16576 c        18962 g        28020 t
ORIGIN      
        1 ttaaaagtat aatttaatta attaagtttg ttttaagaag agaacttttt attaagtttt
       61 tttattttga ggaggaattt gaatgcaaga gctgaagacc cgcagtgccc ggcaccatag
      121 acctaaaaga aaaatggtga tcaataaaaa agcgattgct cctttaattg gaacgagtct
      181 tttagttggg ccgatgatgg ttacagcggt accgcaacat gttcatgcag atgaacaagg
      241 atctcaaaca gtcgctgatc ctcaagcgtt tatcgatcaa atcggctggt ctgcgtcaga
      301 agttgctgca agtaatgact tgtatgcttc agtcatgatc gctcaagcgt tgttggaaag
      361 tgcatatggc acttccggat tagctagtgc gccaaattat aatctattcg gtgtgaaagg
      421 aagctataac ggacaggtcg tttatatggc aaccaaagaa tatgtaaacg gacaatgggt
      481 aacgatgaat gagccattcc gaaaatattc ttcttattgg gaatccttcc aagaccatgc
      541 gaatgttctt cgttcaacca gtttttccac gggcgattat cattatgctg gcgtgtggaa
      601 aagcaataca acaagttttt atgatgcaac agcgtacttg acaggtcgtt atgctaccga
      661 tccaaattat ggggccaaat tgaactcatt gattaatact tataatttaa cccgttttga
      721 tacgccatca acaggttcgg ctacaacaac cacaagtaca acaacaacga ctactacgac
      781 gacttcaacg tcatcatctt caacaacaga tgtaaataca aataatggtc agacatatac
      841 agtcgtgtca ggtgatacgc tatgggatat tgcacaaaaa tttggcatgt ctgttgatga
      901 tctaatggcg aaaaatggca tgtctatgtc cagttattct ttattagttg gtcaagaact
      961 aaacgtttga tttagataaa aaggcaaaga aattaccata tatggtatat ctttgtcttt
     1021 tttttgctag tcaaagacga atcactcgcc ataaatgaac agattgtatt atgataagta
     1081 agaagagcaa agacaaggag gaattgaaga tgacagctat ctataactca gtcaccgaat
     1141 tgattggcaa aacgccaatc gtcaaattaa ataaaatcgt accagaagat tcagcagatg
     1201 tttttgtaaa gcttgagttt tttaatccag gaggaagtgt aaaagatcgt attgctttaa
     1261 gtatgataga aaaagcagaa cacgatggtc tactgaaacc aggtgatact atcattgaac
     1321 ctacttctgg aaatacagga atcggattgt cgatggtagg agttgcaaaa ggatataaag
     1381 taatcatcgt gatgcctgag acgatgagta tcgaaagacg tttattgatg aaagggtacg
     1441 gcgcagagtt gattttgact cctggtgcgg atggtatttc tggatcaatc agagaagcag
     1501 aacgtttggc aaaagaaaac ggctactttt taccattaca atttgaaaat gaagcaaatc
     1561 ctcttgtgca tgagaagacg actggtcctg agatacatca agcatttgga gtaaatggct
     1621 tggacgcgtt tgttgcaggt atcggtacag gaggaacgat cactggcgct ggccgagaat
     1681 taaaacgtgt gtatccgaaa atcgaattaa tcggggtaga accagcagaa tctgctattt
     1741 tagaagggaa agaagcggga cctcacaaaa tccaaggaat cggtacaggt tttgttccta
     1801 aaacactgga tacttctgtg tatgataaag tcctttctat tagtggagac gaagcgatgg
     1861 aaacagctag agaagttggg cgaaaagaag ggattttagt aggcatctca tctggcgctg
     1921 caattgctgc cgcgctaaaa gtcgcaaaag aattaggaaa aggcaagaag gtattagctg
     1981 tggttcccga caatggtgaa cgttatttat ctacagccct ttaccaagag atataaggca
     2041 cttggttttt ctccagatag gtttatgtaa aaaactttta aagatgaaaa gaacacacaa
     2101 aaatttatga tgagaaaaat tatttgaata gagacgtaga atagtgaacg cgactgtttt
     2161 acgtcttttt tcattaaaat acaataaaat tgaattttct tttcatgtta agtggatttt
     2221 aaaatgtaaa aatagtatga taagacgaga ataacaacgt ggggtgttta catggaaaat
     2281 aagttagtcg aagaggttat cgaaaagtta aaaaaagaga acattcgaat cacacctcaa
     2341 cggtacgcgg ttttagaata tttgattgaa catcattccc atccaacggc tgatgagatt
     2401 tataaagcac ttgaacatcg gtttccaaat atgagtgttg cgactgttta caataacctt
     2461 cgcttattta cttcgattgg atttgttcaa gaaatgaagt acggcgatgc ttctagccga
     2521 tttgatttca gttcaaagcg tcattatcat gtaatttgtg aaaactgcgg taagattgtt
     2581 gattttcatt acccagggct tgaagatata gaaatggctg caagcagact gactgatttt
     2641 gagattaatg aacatcggtt ggaattgtat ggactctgtc cggattgtaa agagtcagca
     2701 aaaaaggcgc aaatgtaaaa acagtttcta caagtgttat acctaaaaaa agctagctgt
     2761 gtaaaaaaaa cgctagcttt tttatttttt tgtcttttat tcacaatctt agatataaaa
     2821 gtgaatatta ttaaaataag aatcttgtag atattgggtt cttatgcttg aaaaggaatg
     2881 aaactacaaa caaacctttg tgaattcaca agcattaaac tgttgtatcc gttttatttc
     2941 tgttatagta gttttgtcga aggacaaaat gttaatcgga gggaccataa atgaaagtag
     3001 tagtaattgg ctgtactcat gcaggtactg cagctgtaaa aagtatttta aaaaatcatc
     3061 cagaagcaga agtgacagta tatgaaagaa atgacaatat ttctttctta tcttgcggta
     3121 tcgcacttta tgtcggcgga gtagtaaagg acccagcagg cttgttctac tcaaatcctg
     3181 aagaattatc ttcattagga gcaacagtta aaatggaaca cgatgtaaca gacgtcgata
     3241 cagaaaataa aacagtaacg gcaaaagatc tgaaaacagg agaaacagaa acggtttctt
     3301 atgataaatt agtgatgaca actggttcat ggcctattat cccaccgatt agtggaatcg
     3361 atgcgaaaaa tgtactccta tgcaaaaact acaatcaagc aaatgaaatt attgcacaag
     3421 cgaaagaagc aaaacgtgta gtaattgttg gtggtggata tatcggtatt gaattagtag
     3481 aagcttttgt cgaatctgga aaacaagtga cattgattga tggattagat cgcatcttga
     3541 ataaatatct agataaacca ttcacagata tcttagaaaa agaattgaca gaccgcggtg
     3601 ttactcttgc gctaggcgaa aatgttactg aatttatcac agatgaagaa ggaaaagtga
     3661 aacaagtagc tacaccaaca gatacatttg atacagatat ggtcatttta tgtgtgggct
     3721 tccgtccaaa tacaaaactg atcgaaggaa aagtagatac actgccaaac ggggcaatca
     3781 aagtgaatga atacatgcag acaagtaatc cagacatttt tgcagctgga gactctgcag
     3841 tagtcaacta caatccaagt ggcacgcaaa actatattcc tttggcaaca aatgccgttc
     3901 gtcaaggttt gcttgtggga aataacttga cagatcataa aatggcgtat cgtggtactc
     3961 aaggaacttc aggactttac ctatttggct ggacgatcgg ttcgacaggt gtgaccaaag
     4021 aaagcgcaac tttgaacgat ctagaagtcc aagcgactgt atttgaagat aattatcgtc
     4081 cagagttcat gccaactact gaaaaagtaa tgatggagct agtctatgaa aaaggaacga
     4141 atcgtatcgt aggtgctcag ttcatgtcca aatacgacat tacacaatca gcaaatacca
     4201 tgtctttagc tgttcaaaat aaaatgactg tagaagatct cgcactttca gatttcttct
     4261 tccaaccaca ttttgaccgc ccatggaatt acttgaacct acttgcacaa gcagcgctag
     4321 gcgaaatcga aactgcacaa taaagaaaaa gggttgtgac acaattttgt cacgaccctc
     4381 tgtctttttt tgtgaaaagt acaattagtt gtctttcttc gaattttttg tcacaataag
     4441 cagcaaagat aaagctgtta cataaaagaa caagcagctt tatttgattt tgaaaagaaa
     4501 ggtgagaaag aagatgaata aagaatattt tgtccaacaa gatgcatttt tagaagatta
     4561 taaaagtgct tcgaagaaat taacgatcaa tattgatgag ttggcagatc aagtactctc
     4621 tcagttgaat gaaacgcaag aggaatatta cacgatgcca gcaaatgacg ccaaagataa
     4681 tgtttctcat cgttttcctt tcagtagacg catctactca gatgaaaatg acggcacatt
     4741 gaatacagag tacacatatg aaggtcagcc atatgtgcta gaaactgatg atgatcttta
     4801 aagacagttt ttgaaaaggc cgctcatcct taagtgatga caaaaaatca tcacaattac
     4861 cgatgactgt ctctttacta tcttttaatc agaaaaaggg gttatgacaa gctttgtcaa
     4921 cccctttttt tcttggtttc agattgggtt ttcctacaaa atatttttat ctttgttgaa
     4981 taggaaacaa aaaaacaata taatgaaaga aaaagaaggt gtagaaatgg ataaaagaca
     5041 actgctaata gaagaatatc tggatttagc taatgagatt attcatcgta ataaaccaga
     5101 aatggaaaaa gaatttgcag gatttactct aaatgaacta gaagtgattg aacatatcgg
     5161 gaaaatccct gatcctaacg tgacaaaatt agcgaatgct agttacatga ctagaggagc
     5221 catcagtaag ctaacaaaaa aactgatagc tagagggatc ataaacactt atcaaagcga
     5281 agagaataaa aaagaaatct attttcaatt gacgccaatc ggacaaaaaa taaggaatag
     5341 acacgagcaa ctccatcaaa aatgggtaca gcgagatgct actgtatttg aaaatatgtc
     5401 aaaagaagaa tttgacacag tatttcgttt tatcggtcga tacagagaat ttatacgaag
     5461 tagcaaatga agctactatt ttttattgta gtgataatag gcaaaaatca taagcaaaaa
     5521 tccgaatacc gtcggaagaa atggtgctgc agcataaagc tgtcctgcga ttagcgggcc
     5581 gatgacgcgc gatagagatt gtatactttg ggtaccgccc attacaagac cttgctggtt
     5641 atctgagaca gaattggata gttggccatt gaatacagga gtaaaaatag aatcaccgaa
     5701 accataacag ataatcccaa ataaaaacaa aatcggcagt agaagaattc cagataatgt
     5761 gatgaataga taggcgagga tttcactgaa catccctatt cttgtgatct gctgttctga
     5821 aaattttttg agcaatcgag gcataatgaa caattgcgag acagtatcca agattccaat
     5881 caaagagaag cttaaaccaa ccaacccagc cttccactga aaggcgtcaa tcgaaaattg
     5941 agaaaagata gattgaagag agccagctgc caaccaaaca gcaaaacctg ctgtcaataa
     6001 aggaataacg gatgaaatct tgaataattg ttgtaattgg tgaaaaggtc ttacatggga
     6061 gaaacttaaa tctactgaac gtttttttat tggaaggctt tctagcataa atgtgtagcc
     6121 ataaacggca tttaagaaag taaagagtgc tccaataaat ataggaacag aattccccaa
     6181 ctcagctaat agacccccta taataggacc cagcgttgtt cctataccga ctaaagcacc
     6241 catccatcca aaaactttgg ttctttcatt aggttccgtg atgtcggcaa agtaagcata
     6301 taaggtggag atttctcctg ccgtcaatcc ttcaatgata cgaccaagga acaacatcca
     6361 tatcgaattt cctaaaccaa aaagcaaata tccgatactt gatcctaata aactgataat
     6421 aaggatcgga tgacgcccaa aatgatcact taagctaccg agaatggggg cagataagaa
     6481 ggcagctaga gcataaacag acatgagcaa agtgatatag aaagcttgct gatgtatgga
     6541 gtgagaataa ggcagcgtta aaaacggaag tacaggactg acgatagtaa atcctaatcc
     6601 agtgaataag acagagacgt atccaaaaac tttagaatgt ttatttgtga aactgtacat
     6661 gtaaaaactc ctttatgcaa taatgttaac ttggaaacaa aaatagtgta tagtaatttt
     6721 gtttccttgt caacattcta aggaacagcg aaatctactt aatttattta ttccgaaaag
     6781 tgaagatacg catcaaaaac atgaacaaac aaaaatcaag tagacgacat tgtaaatctg
     6841 accgatcagt gaagtaaata gaaaagaaac ctgttcttgc ttcccatgtt ttttttacgt
     6901 atactgtgat tgagcggagg agaaatcatg ttgaatttag aaaattactt aagcgctgga
     6961 caaacagtca tatctaatct tttacttaaa aattatcata ggatcggtct acaatctgaa
     7021 gaatttttat ttgttctaca gttgcatatg gcacagcttg aaggagatac tttccctgat
     7081 ttgcagatga ttgctatgga tatgggaatc aaacaagatc agatttttca aatactggat
     7141 cgtctcgtaa caaatggatt tatcaagata gaaacgacga ttgtcaacgg taaaaaagca
     7201 gatcgatata atctttaccc catctatgat ctattaggtg agtatttaaa aacacaagaa
     7261 aaaaaatacg aagaaaaaac acaagaaaaa gaaatccagt cagtgtatca attatttgaa
     7321 caagaatttg gacgcccgct ttcttcgatc gagttccagc ggatcggaca atggctagaa
     7381 gaagaccatt atcagccgga aattttgaag ctagcactgc gggaagccgt attaaatcag
     7441 gcttatagtt ttaattacgt tgaccgcatt ttgctatcgt gggaaagaaa aaatctacgt
     7501 acaaaacaac aagtggaaga agaccaaaaa cgaagaaaac agcagcttct tcaaagagaa
     7561 gtagaacagt cagctcaaca agaagaattg ccgaaagtct cactgaaaaa ttggttggag
     7621 gagtagcgga atatgttaag caaacaaaaa acaatggaag ctctagaaac gatgtatgga
     7681 atgtttcccg aagcacatgg agaactcaag cacaataatc catttgagtt attgattgca
     7741 gtgatcttaa gtgcccaggc gacagatgta tcagtgaata aagcaacgcc agatttgttt
     7801 gcctcttttc cgacacccga tgcgttagca gaagcttcaa tcgatgaaat cattcttaaa
     7861 ataaaaacga ttggtttgta tcgaaataaa gcaaaaaaca tcaaagcgtg tgcacagcag
     7921 ctgattgaac gtttcgatgg gcaagtgcca acgtcaagag aagaattgat gtctcttcct
     7981 ggtgtaggca gaaagacagc aaatgtcgta ttgggggatg cattcgggat accagcaatc
     8041 gcagtagata cccacgtgga acgtgtctca aaacgcctga ggatctgcaa actagatgct
     8101 acggtcatgg aagtagaaga aacactgatg cggaaagtac cacaagaatt atgggtaaag
     8161 acacatcata cactgatttt ttttggtaga tatcattgca cggcaagaaa tcccaagtgc
     8221 gaggtatgtc ctttgctatc aatatgtcaa gatgggaaaa atcggatgcg tctaaaagaa
     8281 aaggcgctaa aaaagaaacc acgcctgtag acgtggtttc tttaattgca aataaatgta
     8341 tgaattatct tgttattatg gagaaaagct gtttcgttta tcttttttgc atgaaaaagt
     8401 aaatgtacat actcagtagc taggatggta acttattaca atttattgcg ataaaaataa
     8461 aagttgttat ttttattggg aaaatacccg atgtagaaaa taatgccttc caatatacct
     8521 ataaaataag tccagtattc caaaggatta tataaataag tgacgtatct atctaaaggt
     8581 gtaataaaaa tgataagtga taataaaaaa ataccattta taactaaaca taaactaatg
     8641 gatttgggcg gtttcaggct catcagaaaa atatagattc tggtgaacaa caaccataaa
     8701 aaagtgagaa tcattgcatg agagcttctt tccataaatt ttcctttaag agtagtgctt
     8761 aaaagtacag aaaaaataat aaatgcaagg ataggagcaa ttaaagtaaa tttatttaat
     8821 aataaacaaa atttttcagc ttttttattc ttggaataac gaggtattgt tttcgataaa
     8881 aaaagatggt gcagacaaat tatgataatt aagcatacta tcagataact caaaatgaag
     8941 ccttctttca aaaaattatt caaaaccata tattgtattt tttatttgat aataacaatg
     9001 ggattattaa gaagtggcac taaataaaag actagtgtgt atttagccga tttaattata
     9061 aacaagtaaa tggataaagt atatgtaatg ataaaagaaa aatggatttt ctaattttta
     9121 agctaaatgt tagatcacac atgtatatgc tggatagtct tataaaaaat catgtaatac
     9181 agcttggtac atgattttta tttgtagctg ttttttacat agacacaaaa aagaaaccac
     9241 gcttgtagac gtggtttctt ttttactatc tgtttccaga agctgaggtg ctgctggatg
     9301 gtgtatggtc atttgcagca tccgctccgc tagttgctgg ctcactggaa gaagcaggag
     9361 gagcagatga agacggtgtc gagctttctt cggatgaaga agaaggaacc gttgttgaag
     9421 acgctggctc gcttgtttct gctgtcgtgc tttcttttga ggactcagca gttgattcgc
     9481 tttgactagt agagctagag ctttgagtcg ttgtctctgt tgttgagctt ccaggagtct
     9541 ggacatagct ggaagatgga atagttgttg atggtgtaat tgcattcgat ctagctgtat
     9601 attgatcttt gtagtacagt tcaccgccca cacgaattaa tccgcttggc atctcccagt
     9661 ctgtatttgt cacgttagct gaaacatact gcataagctc gcgataaaca tcagaggcta
     9721 cgtgtgacga ttctgaagtg acaggtgtca tttttttatt gtaacctgtc cacacggaaa
     9781 tcgaataatt tggcgtatag cccgcaaata ggatatctgg atacacccca gaagaaatac
     9841 caagttttgc atactcgtca tctgtgtaat tggatgttcc ggtcttaccg gcttgatata
     9901 acccagcgat ttgcgcattc gtaccagttc cttcagtgat cgtatctttt aaaatgtctg
     9961 tgatcatata agctgtttct ggagacatgg ctgtttttcc atctggctca taagtttctt
    10021 ctgttccgtc ttggaagacg attttattaa catattgagg tttgtaataa gtccctccat
    10081 tggcgaaagc cgcataggct gctgccattt ttaaggaaga agctccgtat tttgtcccat
    10141 cttgttcttc cgtattacta gagatcgcat tcgattgatg gatcgtactg tattcgatcc
    10201 caagattttt taagaaagaa gccactttat ctgaaccgac ttcattgaat aatttgactg
    10261 ctggaacatt acgggataag taaagtgctt ggcgtaaagt aatggtgccc atatattgat
    10321 tatcccagtt tcctacagga gtcgaagtcc cttcatagtt atacggtgcg tcagtaatag
    10381 tttttccagt agaatatttc aaatattcaa aagctggtcc gtaatctgtg acaggtttca
    10441 tcgtagaacc aaaatcacgg gaagtattca ctgctaagtt gttgcctaaa gttacgtctt
    10501 ctgcgatgtg acgtccgcca atctgggctt ttactttccc ggtattggtg tcgataaggg
    10561 tggaggcaac ttgcatctcg tcgtctgggt aagagacata ctgatccgtg ttgacgatat
    10621 catatagttt cttttgggcg tccagatcca aattcgtata aatctctaaa ccgtctgtgt
    10681 agacattctt atctgttttt tcttgtactt cattgatgac ttctttgaca taattatcga
    10741 cgatcttagt attgtcatcg gattgagtta gttcttgcag accatccgtt accggtacat
    10801 taacagcttg atcatattct gtttgagaga ttttttcgtt ttgaagcatc gtatacaaaa
    10861 cagtatctcg tcttttcttc gcctgatctg gataaacata aggatcatag gcggaagggg
    10921 cttgaggcat cccagcaagc aatgctgttt gcggtaaaga aagttctgat aatttcttac
    10981 caaagtacat ctcagaagct gtctccatcc cgtacaagcc gtttgacata tacactttat
    11041 ttacgtagta ggtcaagatc tcttgtttcg attttttttg ttccaatctt acagccatcc
    11101 acgcttcttg ggccttgcgt ttcagagtct gatcctcggc actggtggag aagaaggaaa
    11161 gtttgatcaa ttgctgtgtc aaagtacttc cgccttgcag tccgccagag gtaaagttag
    11221 agagggcaga accgatgatc cgaatagggt caaccccgat gtgtttataa aaacggcgat
    11281 cttctaccga cacgatcgca tcttctaatg ttttcggtaa ttcattcgca gaaatttttt
    11341 cacgcttttc tgcgcctaaa tcttcgaata gttcgccatc ttgtgtatat agtttggaag
    11401 atacggtagc gtctaatttt ttatccgtca attcgggcgc atcttttgcg taataccaga
    11461 aaagtcctac acctgctaaa aagaggatac agaaaaatga gagtattcct aaaaggatct
    11521 tgataaataa tcctttttta tgtgttccag aggactttcc agaacctttg ccagaattct
    11581 tcttcacact ctttttagga gtaggctgtt tctgtcgtct agagcttcta gtttgttcat
    11641 ttgccattct tgctttctcc ttcaatagat aatgttgata aatagaagtc aaccgcgtcc
    11701 aaataaggaa tccgcggaga aataccagtt ttgatctcta ttcctagttc ttcgatcaaa
    11761 gataaaggta gtgatttgcg tcctttttcc ttttgggcgt cccagtaagc aatcaaatga
    11821 acactattca aataaaaaca gcgcttcaaa gacgaaaacc ataacaagac aaagcagacc
    11881 ccattttgtt cgaggcatgc tttcatatgt tcgatttgat gggcatggaa gtttttaaag
    11941 ggaaaagatg ttttgttctt ggtttctttt gcttcaaagt ccaagtaaaa gcctcgatag
    12001 acgccattat agtctgtagt tgaggcttgg gtaaagtagg cttccttgat cacagcagca
    12061 cttctcttag gatagtctac tttcacgatt tggatagggg taggcttttt atggataacc
    12121 gccaacccgc gggaaagata atatttatta ctttcattga ttgcttcttc aaagcgcatc
    12181 ccccgattgc cgaattcagt gatctgattt cttttttctt gaattttttc gacgggagag
    12241 gcattcttat aataaggttg gccatttgga tagcgcaata ccatctttaa tcacactcct
    12301 aggccacatt atagcaaaaa agcggataga aagaaaagga atctgagatg gataagtcaa
    12361 aagtaatcta tgtgtctggg tatcgaagtt tcgaactcgg tgtttttaaa gaaaatgatc
    12421 ctaaaatcca agttgttaaa aatgttttaa aaaaagaact ccatcagtta gcagaagaag
    12481 ggctggaatg ggtacttgtc agtgggaatt tgggcgtcga actatgggct gtgcaaatag
    12541 tagcggaatt gaaagaagaa tatccggaac tatctcttgg tattttgtat cctttttctg
    12601 attttggtaa taactggaat gaagggaacc aagaaaaaaa acaattagca gaacaattag
    12661 cagattatgt cgaagctgtt agccatcaac cctaccaatc acctagtcag ctaaagaatc
    12721 acactcgttt tatacttgag catacagtgg gctgtctgct gatctatgat gaagaattcc
    12781 ctggaaaaac gaagtatttt tttgaagatg cacagaatta ccaagcagat catccctacg
    12841 aaattcggct gatcagtatg gacgatcttc aaaattttag tgaataatcg atattgtgtt
    12901 tgatttatca acttttttcc tatacaatga aaaaggtaga aatgttatta cgtaaatgaa
    12961 aactagaggt gtaaatatgg cgaatttggt ttatagtcct aaagacattt tacaacaaga
    13021 attcaaaaca aaaatgcgcg gctatgatcc cgttgaagtt gatgaattct tagacaatat
    13081 catcaaagat tatgaaactt acagcaaaga actccttgct ttacaagaag aaaacgatcg
    13141 attgagtgca aaagtagctc aattatctaa aactcaagga gcagctcaaa cacgcgtgca
    13201 acaaacagag gcaccaaaga gcgcagcagt gaccaatttt gatatcttaa aacgcttgtc
    13261 aaatcttgaa cgtgaagtat ttggtaaaaa gcttgatcaa caagcaagtg cagtcaaacc
    13321 agcacaacct aatccaaata actacacaaa tgcagacaca agcttagatg ataatgaaaa
    13381 aacacgtcaa ttttaatttt tgattaatga atttgcggtt ttcgggtaat cgcggtctga
    13441 cttgatcagg ctgaggaaag tccatgctcg cacaggctgt gatgcctgta gtgttcgtgc
    13501 ttagcgaaac cataagctaa ggtactcttt ggagtaacgg caggaaaaca agctaaggct
    13561 tcggctatgc ttaagtatcc ttgaaagtgc cacagtgacg aagcggctgg ggaaacccat
    13621 ccggtggaac gcggtaaacc cctcgagcga gcaacccaaa caatggtagg ggcgcttttt
    13681 gtctggaaga agaacaggac aaaaaggcag tataactgca gatagatggt taccgctttg
    13741 ttttttctcc ctggaaaagc aaagtacaga acatggctta tagaaaaccg caaacatcag
    13801 ggaattaggt cgtgacaaaa gtaatgcgtt ataagaaaca agaaaaacat ttgaaagcta
    13861 tttttcaata cttcagatga atcgttctta ttacaaaaca aacttttgta tcaccggtta
    13921 ttcggaattt gagtaaaagt gtggggtagg actgatgtcc cacctccttt ttttataaat
    13981 aaggagattg tgtatggaaa aaaacaggaa gttcaacctc gtcgcaactg cggcaagcgg
    14041 gctagaagca ctggttggta aagaattaag agatcttgga atcgaatgcc aagttgagaa
    14101 cggacgtgca cgttttgaag gaacaatgga aacgatcgct acagctaatt tatggttacg
    14161 tacagcagat cgagtaaaaa tcgtggttgg ggaattcgat gcgtatacgt ttgatgaatt
    14221 gtttgaaaac gtaaaagcat tgccttggga agattttctt ccactagatg cagcttttcc
    14281 tgtagctgga aaatcgatca agtcgaaact atatagcaca cctgattgcc aagcgatcac
    14341 aaaaaaagca atcgttgaac gtctccgtac ctattatcac cgtccagctt ctgtaccact
    14401 gacggaaact ggcgcacatt ttcagttgga agtagctttg ctgaaagacc atgtgatggt
    14461 aactctggat acgacaggac caagtttatt caaacgtggt tatcgcttag aaaaaggtgg
    14521 agcaccattg aaagaaaaca tggcagcagc acttattatg ttgacaaatt ggcgaaaaga
    14581 ccgtccattt tatgatcctg tatgtggttc aggtacgctt tgtattgaag cagccttaat
    14641 aggccataat atcgcaccag gattcaatcg tgagtttgct tgcgaatctt gggattggtt
    14701 cagtcaagaa atcatggaaa atgttcgatc agcagccgaa gaaaaagcag attatgatat
    14761 tgaattagat attactggtt cagatatcaa tggacggatg attgaaattg ccaaagccaa
    14821 tgcagaagaa atcggattag gctcttcgat tacctttaag caacaagcag tcaaagactt
    14881 caaaacggat aaagaatacg gggtcatcgt ggctaatccg ccttacggag aacgtctagg
    14941 agaagaagaa actgttcgga aattatataa agaaatgggc gatgtattcc gtccattaaa
    15001 aacatggagt aaatatatct tgactagcga cttagcgttt gaggaatttt acggacaaaa
    15061 agcaaccaaa aaaagaaaat tgtacaatgg tgcattgcgt acggatctat tccagtattg
    15121 gggaactcgt ccgcctagaa aaaaagagaa agacgaataa tcttactaat tgattcattc
    15181 aattggatgt ttggaggaat agggaatgaa agaaaaagag tttttacaag aagtaaaaga
    15241 aatcaatctt ctacaacaag ctttaggaat cctcgattgg gatacacaaa caggtatgcc
    15301 tgaaaaagct agtgcatacc gaagtgaagt agacagctat ctttacagca tttatttttc
    15361 aaaaaaaatc ggtccaaaaa tcaaagaagc cattcgctat ttttctgaac atccggaaga
    15421 attgtcggaa gttggaactg ccgtttttgc caaagtaaaa gaagagtatg atttggaaca
    15481 tcgcgtaccg gaagatctta tgagaaaata tacagctgcc gtttcttcag cacacggaca
    15541 atggcaaaaa gctagagaaa cacaacaatt ttctgatttt caacacgcat tgaatcaaaa
    15601 tattgaatta acaaaacagt tgatacctta ctggagaaaa gatgaaaaga cgaattatga
    15661 tgttttgctg aatcaatacg aacccgggat gactgtcgaa atccttgata aggtatttga
    15721 tcagcttaaa gaaggaattc tttcgatccg ccgtacgctc caagaaaaag ggactgaacc
    15781 tgaaacagac tttttaaatc ggagaatgac gaaaaaacaa cagaagagat ttgtagtgaa
    15841 agtaatcgaa caacttggtt atgattttag tcgtggtcgt ttagatgaca ctgtccaccc
    15901 atttatgact ggaattaatc cgaatgatgc acgaatcacg acacgatgga acgaaacaga
    15961 tttcaaaatg gctgtttttg gagtgatcca cgaggctggt catggtatgt atgaacaaga
    16021 catcgatgat aaatatgcgt atacaccggt ttatgaaggt acatcaatgg gtatccatga
    16081 atcacaatcg ttgtttaacg agatcattat tggaagtagt cgcagcttct ggaaaaagca
    16141 atatcctttc tttcaagaat gtgcagaagg aacattcgat gatattcctt ttgacaaatt
    16201 ttatcgttca ttgaaccata caaaatcaag tttggttcgt atcgaggcag acagtttgac
    16261 ttaccctctc catatcatca ttcgttatga aatcgaaaaa atgattttca atgaaggggt
    16321 agatgttgct gaactacctg aaatatggaa tcaaaaatat gaagagtatc tgggaatacg
    16381 accaacaaat gatgtggaag gcatcctaca agacgttcat tggtcaggtg caagctttgg
    16441 ttattttccc tcttatgcat tagggtacat gtatgcggca caattacttc attctttgca
    16501 gcaagaaata gatgtggaaa atgttctagc gtcagacgat tattctccaa tcaaaaactg
    16561 gatgtcagag catatccatc aatatggtgc atctagaaaa ccaaatcaat taatcatgga
    16621 tgcaacacat gaaccgctta atccgcaata cctgatcgac tatatgaaag acctgtattt
    16681 taatgtatat caggtaaaat aaaatacaat ttacaaagat agctgaaatt catctaatct
    16741 atagatgttt tcagctattt acttaagaaa gaagtggaac gaatggataa attacaagtg
    16801 atcgctgatt atagtatgga ccaattagcg catgatcaaa caggacatgg gagcgatcat
    16861 accaaaagag ttgtcaagtt agctgaaaaa attttggaga cggaaccgca ggcagaccgc
    16921 tttgtgacgc ttgcagcagc ttacctacat gatacgattg atgataaagt cgttaaaaat
    16981 gagaacgagg caaagcaaca gcttcgtgta ttccttcgct ctttgccaat agtagaggaa
    17041 cagatctcga tgattttttc cattatcgaa aatatgtcct tttctaaaaa tctctctgaa
    17101 gcggccgaac tatctctgga aggtaagatc gttcaagacg ctgatcgtat cgaagcatta
    17161 ggagcaatcg gcattttgcg tactgcttat ttcggcggtg gacatgggca cccgattttt
    17221 gatcctgaac tgtatccgca aacattcaag gacaaagaaa attaccgaaa aggcacgaca
    17281 gtgatcaatc atttttatga aaaactgttc ttgctgtctg ataaaatgaa tactgattac
    17341 ggatatgaag aagccaaacg aagagaaacc ttcatgcgtg aattcttaga agaatttttt
    17401 ctggaatggt ctattgacga tgaaaatttt actccagaca attggatcaa aaataacaaa
    17461 aaatgatgga ggttgtgaca gaagtggtca gctccaagat atttcggatc attttccgta
    17521 aaagttactt ctggaacact gtttaccaag aggtcgtgag cctgccgttc agctccgagt
    17581 cacaaggaac aatttcaaaa aatagcttct cgtgtttgat gaatattgta acttgtgatt
    17641 cggaattggc aggagaacac cgtttattcg gaagaatagg actgtgacaa gacttttgtc
    17701 acagtccttt tttcgaattc aacaagtaaa acttttaaga aatattcaga gagtaaaatt
    17761 aaaaattgct gataaacgaa gatggaaatt ttatgtattt ttattggtct atgcaataaa
    17821 taaattaaat attcagaata caattgattt taacgaaagg gcttctatca atggttgtgt
    17881 tgtatttatc tgcccaggta gagtaaaata gatcattatc taaaaggtaa ttgttcatac
    17941 tatataagtc atttgaaagt aaaaagatac tgtaaataaa aaatgtggtt aaataattct
    18001 cataatcagt gtaagcgtta tcaaaaatat agcatacttg ctttgtagat gaatgacacg
    18061 atatatgttt ggtatgttct gcaagacaga tgaatatgga ggtggagaca atgtcttcca
    18121 aagaactaac aatgtattta gagaaagaat tggatcaact gaaagaaaag gggttataca
    18181 atacgatcga catactagaa agtgaaaatg gtgcttgtat catcgtagac ggaaaaaaga
    18241 tgatcaatct agcatccaac aattatcttg gatttgccaa tcgcgaagaa ttgaaaaaag
    18301 catgtattga agcaaccgaa acttatggtg taggagcagg agcagtccga acgatcaatg
    18361 gttcgctgaa aatccatcag caattagaag aaaaaatcgc agaattcaaa gggacagaag
    18421 cagcaatcgc ctttcaatct ggattcaact gcaatatggg cgctatctct gcagtaatga
    18481 caaaagaaga tgctatctta tcggatgaat taaaccatgc atctattatt gacggttgcc
    18541 gcttatccgg agcaaaaatc atccgaatta aacatcaaga tatgaaagac cttgagaaaa
    18601 aagcaaagga agcgatagaa agtaagaaat ataaaaaaat catgtatatc acagatggtg
    18661 ttttctccat ggatggagac attgcacgat tgccggaaat tattccgatt gttgaaaaat
    18721 atggactgat cacctacgtc gatgatgcgc acggctcagg cgtaactgga aaaggtgcag
    18781 gaaccgtcaa gcattttgga ttaagcgaca aaatcgatat gcaaatgggc acgttatcga
    18841 aagctatagg tgttgttggg ggttatgtag ctggatcgaa aacactaatc gactggttga
    18901 aagctagaag tcggccattc ctattttcta cttcgctaac tccaggtgca gctgcagctg
    18961 cattggcttc gattactttg atgcaagaac atccggaact tgtcgaaaaa gtttgggaaa
    19021 atgctaatta ttttaaagaa gaattgaaaa aagtaggcta taacatcggt atgtctgaaa
    19081 cacctattac tccagtcatt ttgggagacg aaaaagtgac tcagactttc tctaagaagc
    19141 tgatcgaaca tgggatttat gctaaaccaa tcgtttatcc aactgtgcct cttgggacag
    19201 gcaggatcag aaatatgccg actgcggaac atacgaggga aatgcttgat gaagcagtag
    19261 cagtttatca aaaaatagga aaagaaatgg aaattattta actaaattta gttttttcgt
    19321 ctatttaaaa ttagagaaca tgttcacgca tatattgtta atcatctgaa gatagtagta
    19381 aaaaagatca gggaggatca tcatgaaaaa agtattggtg acaggatgtt taggacaaat
    19441 tggttcagag ttgatcacac ggctacgtca agaactaggc agtgaaaatg tcattgcgac
    19501 agatatacgc atgcctgaag tcattgagaa aaacgatatc tttgagatcc tagatgtaat
    19561 ggattacgaa aaaatgcgca gcatagcaga acaatatcaa gtggacacat tgattcattt
    19621 ggctgctctt ttgtcagctg ttgcagaagc aaaaccacaa ttagcctgga atttgaacgt
    19681 gactgggctg gtcaatgcat tggaagtcgc tagagaattc cagctgaaat tttttacgcc
    19741 aagttcaatc ggcgcgtttg gtccaaatac tccaaaagat cataccccac aagatactct
    19801 tcagcgtcct acaaccatgt atggggtaac gaaggtagct ggagaactac tatgtgatta
    19861 ttattatgtg aaatatggcg tagatactcg tggtgtccgt ttccctggat tgatttctta
    19921 tgcaaccttg ccaggaggcg gtacgacaga ctacgcagtc gatatttatt attcagcaat
    19981 caaagaagga aagtacacta gtttcattga tcaaggtaca tcaatggata tgatgtatat
    20041 gccagatgcg atcgatgcca ttataaaact catgaatgca gatcctgatc gattggttca
    20101 tcgaaatgcc ttcaatatat cggcgatgtc ttttgacccg gaagagatca aagcatctat
    20161 tcaaaaaatc ataccagatt ttgaaatgga ttatgatgtg gatcctgtac gtcagtcaat
    20221 agcagaatct tggccaaatt cactagatgt ttcttgtgcg caagaggaat ggggcttttc
    20281 gccgaaatac gacttagatg tgatgacaaa ggatatgttg gaaaaactga aagaaaaatt
    20341 ggtagagacg gtataaaaca agaaccacat ccaaaaagtt ttttatactt ttgggattgg
    20401 ctatggtatg aaaggggaca agggatggaa gaatatcaag ccacgccagt aaaagaagta
    20461 aaaatagaaa atgaattaaa acgtacgatg ggatttttta ctgctttatc gactgttatg
    20521 ggcacagtga tcggtgcagg ggtatttttt aaagccgcaa gcgtagcaga agttacagga
    20581 tccgccagtt tgcatatgtt ttcttggttt ctcggtggta tgatttctgt ctgtgcagga
    20641 ctaacaggag ctgaactggc agctgctatt ccagaaacag gagggatgat taaatatatc
    20701 gaacggattt acggaaatac agcagctttt ttattgggct gggcgcaagt cgtgatttat
    20761 tttccagcaa atgtagcagc cttgtcgatc attttcggta cacagttcgt caatctattt
    20821 ggtctttccc agtcaatgat tgttcccgta gctgttacag ctgctgtatc gatcctgttg
    20881 atcaattttt taggttctaa agctggaggg gcttttcagt cgattacgct tgtctgcaag
    20941 cttattcctt tgtttgtgat cgtcattttc ggtttgttcc gacaagaagg agtagacttc
    21001 caactgtttc cgatccaagc aggagaaaat ctttcatttt tctcagcctt gggagcgggc
    21061 ttgctagcaa cgatgttcgc atacgacggt tggatacacg taggaaatat ttcgggagaa
    21121 ttgaaaaagc cggcgaaaga tttgccaaaa gcaatttctt taggaatcat cggaatcatg
    21181 atcgtttatt tattagtaaa tgcggtgttc ttaagaaccg catctatcga cggagtcgca
    21241 ggaaacagca atgcggcaag tgacgttgca aaaatgattt ttggcggctt cggtggaaga
    21301 ctggtcacag ttggtatctt gatttctgtt tatggaacca tcaatggcta tacccttact
    21361 ggaatgcgtc tcccttatgt tatggcaaaa gaaaacaacc tgccttttag caagctcttt
    21421 gctaaacttc atgataaaac taaagttcct gtagccgcag gtatcttaga gctagtcatc
    21481 gctattggca tgatgatgat cggtggtttt gatacactaa cggatatgct gatctttgtc
    21541 atttggattt tttatacgat ggtctttgtt ggtgtcattc ttcttcgtaa aaaagaacct
    21601 gatttgtttc gaccatacaa agttccaatg tatccgttta ttccactagt agcaatcatt
    21661 ggagggactt ttatcgtatc aagcacgctt atcactcaga cctttttagc ttcgatgggg
    21721 atagtattga cacttgctgg tatcccgatt tatctctatt taaaacgtag atagctaaaa
    21781 cattcgaatt ttattagaac tgcacttctc tgccaaacaa aatattttat ttaaatgagg
    21841 agtacgtgga agaaatgtca aaagtactcg ttaattttga tcacgtaaat gtcgttttgt
    21901 ctacgatgaa atagcaaagt aaaagacaga aatcgggctc aaccgatttc tgtcttttac
    21961 tttgatttgc gtaactgaac tattcggcgt acgccgcgat aaatatttaa taaaaccaag
    22021 caaccagaaa tgaaaatggg aagccaaatc agatagacag ggtagccaag cactttagac
    22081 aaagcatgtg gagtcatttt atccatttca agaagtgctt gctgccaagt ctgaagattg
    22141 aagactagca tgaaacccga aataatcagg agaataaatc ctaaataatg aattgtagtt
    22201 ctttttctta cataagcgat cgtagcataa aggaacagta aaaaaatagg tacgccaaaa
    22261 acaaagtaca tattcagcat ctcgacaccc cctttgtgtt tatcttactt tactttgaaa
    22321 gaaatgagaa ataatagcct cgttacttgc ttgaaacagc aaaggactgc taagataaga
    22381 gagatacaaa gaatcaagga ggagtcatat ggcaagttat atcgaaccga ttcgttttgc
    22441 gctgattgtt tttccatttt tagccttagc gatctcatct gtttttttta tttatgaata
    22501 tcgaaagtat ggaacatttc tagtaacaag ggcagttatt ttgtattctt tcgtttttta
    22561 tttattgtgt gcgtattttt tggtgatctt accacttccg gcaaaggaga cggttgccca
    22621 aatgacaggg ccaaagatgg agctgcattt atttgcttca tggcaacatt tcagagacaa
    22681 cacggtattg gtcttgtctg aacctagtac ctacctgccg gcaatgaagc agagtgtatt
    22741 tttggaacca gcttttaatg ttttattact cataccattt ggcatttatt taagatatta
    22801 tttccggctt tcttttatga aaacgatgat tgctagtttc tgcctttctc tattttttga
    22861 attgacgcag ctaagtggac tttattttat ctacccaaga ccataccgct tatttgatgt
    22921 gaatgattta gtgacaaata cattgggtgg aatcattggt tttgtcgtta cgccgttatt
    22981 tacatttatg cttcctacaa gaagacgaat ggatgaactt tcttatgaaa aaggaaagac
    23041 tgtctcattg actcgacgct tggtcgctta tttgatcgac tggacatttt tatctcttat
    23101 taatttggta gcggcgattt tctttaaaat tgcgttcgat tcaggggcag ttacttctca
    23161 tatctggtgg cttttattag aagtaggggt atactttatg attgtaccgt ttataaccaa
    23221 tggatggaca cttggcaaaa aaattgtgcg tatccgtttg gtggaagaag accgcgaacg
    23281 aatttcttta aaagcattga tgatccgcta cgggtatctg tactttcttt tttatggttt
    23341 aagctggttg gggcagttgc ttcagcaaat catgaattcg acaacgaata cggtgttgca
    23401 gatgagtgca gtattcattt ctctcggtgt cttgttacta caaggtattt ttattttaaa
    23461 tatcttgcta tcatttttga gaaaaaaacg gattctgttt tatgaaaaag ctagtcacac
    23521 atatactgtt agcacgattg gccaaacaaa ggatgatgat tctcgaaatt agctgagcaa
    23581 aaacgctttg tctgtgttat aatgccaaag tcgtgataat ttaaagagaa gaggtgcacg
    23641 aatgaataat tttgatatat tccgcttcaa accgtttatc aatgaagcgt taaccgcaaa
    23701 gggcttcaaa cagcccacag aggtccagga acgtttgatt cctttaattg caaaaggaaa
    23761 aaatatcatt gggcaatctc aaactggtac aggaaaaaca catacgtttt tgttgccttt
    23821 gtttgaaaaa atcaatgcag aaaaagagga agtacaagtt gttattacag ctccaagtag
    23881 ggaactagct gcacagatct atgaagcagc tacacaaatc gctaaatttt ctacaccgga
    23941 gatccgtgtc tcgaattttg tcggcggtac agacaaacag cgtcaattaa atcgtctgca
    24001 tcaccaacag ccacaggttg tagtaggcac gcctggtaga atattagaca tgatgaatga
    24061 acaagcgctg aagattcaca ccgcatttgc atttgttgtc gatgaagcgg atatgacatt
    24121 ggatatggtt tttttaaatg aagtagacca aattgcaagt cgattaccag aaaagctgca
    24181 gtttttagtt ttttctgcca ctattccaga aaaacttcgt ccattcttac gaaaatacat
    24241 ggaaaatcca atcattgaag aaatcaaacc aaaagcagtt atttctgaag acattgaaaa
    24301 ttggctgatt tcaacaaaag gtaaagatga gaatcaaatg atttatcaac tgctgacaat
    24361 tggtcatcct tatttagcaa tcgtatttgc caatacaaaa cagcatgtgg atgaaattgc
    24421 cgattacttg aaaaaccaag gcttgaaagt agcaaagatt catggagaca tcaccccaag
    24481 agagagaaaa cgagtgatgc gacaagtaca gaatctggag tatcaatatg ttgtagcgac
    24541 tgatttagct gctagaggga tcgatatcga aggtgtctct catgttatta atgcagaaat
    24601 tccagaagat ctagacttct ttatccatcg tgtcggacgt actggacgta atggcttaaa
    24661 aggaacggcc attacgttat attcgccaaa tgatgagcaa gccattgatg cgatagaaaa
    24721 attgggcgtc tcatttgaac caaaagaaat caaaaacggg gaaatcgtca aaacatacga
    24781 tcgtaataga cgtgccaaac gagaaaaatc aaaagatgta ttagatccca cgctgattgg
    24841 tcttgtgaag aagaaaaaga aaaagatcaa gcctggttat aagaaaaaaa tcgattgggc
    24901 aattgcagaa aaaaataaaa aagaacgcaa aatcgaacgc agacagcaaa gccgtactgc
    24961 tcgtaaaaat aagaaaaatt cacataaata aaaaacaaga ggttgtgaca aaaggcgtgt
    25021 cacaatctct tgtttttaag ctagactttt tttcattccg atacaaagta cttcatcttc
    25081 aataaaaggt tctgtgcaag tatgataccc ttgtttttca taaaaatcta cagccgtcat
    25141 ttcagctact acataactag tctgacaacc ttctgaaacc gcccgttttt cagcagcttc
    25201 aagcaactga ccgcctaacc cttttcctcg gtattctttt gctacgcaaa atcgttccgg
    25261 cttgaagcaa gtaggtgttt tcttttcata acgcaaagta gctactggtc ttttttgatc
    25321 aagaaataga taataggttt gttgcgggat atcttttccg tcgaattcta gttctgcagg
    25381 aatcccttgt tctaatacaa aaacttgata tctcaaaaag cagctagctg cgcgtatcca
    25441 ttcatccgct ccaactattt ttttcatcat attcctcctt gtcatgaata gcagagatgt
    25501 attgattcct ttattcttat cgtaccaaaa atcgtggagt atgaccaaaa ggaaatgaaa
    25561 ataaaaattg agatattgac agtgatcaat agaatgcgta tactaaataa gtacgaaaag
    25621 gatatgattt gttttttaag actcacagaa aattctttct tggctgaaaa aagaatagtc
    25681 actaatcaaa ttgctccctt tttctatttt ggtagaaata ccgacgcagc tcagcgttat
    25741 cgagtttaag aggtaatgat tacacatcat tgcaatcaag gtggtaccgc gagttttcgt
    25801 ccttggctgt gatggtgttt ttttgtattt aatttataga aagcaggtaa tgacatgaaa
    25861 caattaagta gtgcacaagt tcgacaaatg tttcttgatt tcttccaatc taaaggacat
    25921 acgattgaac ctagtgcgtc tttgatcccc gtgaacgatc caaccttatt gtggatcaac
    25981 tctggtgttg caacattgaa aaaatatttt gatggttcag ttgtgccaga aaatccaaga
    26041 atcacaaatg cccaaaaatc tattcgaaca aatgatatcg aaaatgtcgg taaaacagca
    26101 cggcaccata cgatgtttga gatgctgggt aatttttcga ttggtgacta tttcaaaaat
    26161 gaagccatcc actgggcatg ggaatttttg actgatccaa aatggatggc attcgatcca
    26221 gaaaaacttt atgtaacagt ttatccaaaa gatacagaag caaaacgcat ctggcatgaa
    26281 gaagttggcc tgccactaga tcacattatt gatgtagaag acaacttctg ggatatcggt
    26341 gcaggcccat gtggtccgga tacggaaatt ttctatgacc gtggagaagc ttataacgat
    26401 ttagcagaag acgatcctga aaactatcca ggaggagaaa atgaacgtta tttagaaatc
    26461 tggaacttag tattttcaga attcaaccat aagcctgata acacatttga accacttcct
    26521 cataaaaaca ttgatacggg gatgggattg gaacgcatgg tctcaatcgt tcaagatgcg
    26581 tcaacgaact ttgagacaga cttgtttatg cctattatcc acgccgtaga aaagctaagc
    26641 aaacaagtaa aatatggtga aaacccagta acagatattt cttacaaagt aatcgcagat
    26701 catattcgcg ccctttcatt tgctattggc gacggtgcat tgccatcgaa tgaagggaga
    26761 gggtatgtct tacgccgttt gcttcgtcgt gctgtcatgc atgggaaaaa attaggtatt
    26821 gaacaagcat tcttatatcg tttagtccct gttgttgggg aaatcatggt aagttattat
    26881 cctgaagtac ttcaacaaaa agaatttatt gaaaaagtta tccgtacaga agaagaacgt
    26941 ttcaatgaaa caatcaatga aggtttggaa atcttaaatc aagtgatcca agaagtaaaa
    27001 aacgaaaacg ggacgactct agcaggaaaa gacatcttca aactatacga cacatatggc
    27061 ttcccggtag aattaacgga agaggtggca gaagatgaag gactgaaagt tgatcatgct
    27121 ggttttgaaa aagaaatgga tgcacaacgc gaacgtgctc gctctgcccg cagtactgaa
    27181 aaatcaatgg gggtacaatc tgctttattg acagatatca aagtagaaag taaattcatt
    27241 ggatacacag aacaagaatc agatagtcaa ttattggtca tcgttaaagg tgagtctctt
    27301 ttgaatgaag tcgcagaagg tgaagctcaa atgatctttg atcaaacacc tttctatgca
    27361 gaaatgggtg ggcaagtagc ggatcaagga tggatcatga atgaagtagg cgaaatcgta
    27421 gcagttgtta cagatgttca aaaagcgcct aatggtcaat ttatgcacac cgtagaagta
    27481 aaagcaccaa tgagtgaagg acaaacctat catctattga tcgatcaatc gatgcgcaat
    27541 cggatcatca aaaaccatac agcaacccat ctactgcaca aggcattgaa agaagtactt
    27601 ggcgagcatg caaatcaagc tggttcttta gtcgctcctg gacatttacg attcgacttt
    27661 acccactttg gtcaaatcac ttcagatgaa ttagctgaaa tggaaagaat cgtcaacgaa
    27721 aaaatctggg aagctatccc agttgaaaca attgaaacag atatcgatac agccaaaaac
    27781 atgggtgcaa tggctttgtt tggtgaaaaa tacggacatg acgttcgagt agtaaatatt
    27841 ggcggctggt caatcgaact gtgcggaggc acacatgtag caaatacaga aaacatcgga
    27901 atcttcaaaa tcgtttctga atcaggaatt ggtgcaggcg tgcgacgaat cgaggctgta
    27961 acaagtaaag aagcgtacga actacttgaa gaagaagaaa aacaactaaa acaagtagca
    28021 acaattgtta aatcacctca attgaaagaa gttgtttcaa aagcggaaca attgcaacag
    28081 caagtaagag agctacaaaa ggaaaatgaa caattagcta gcaagttagc gaatcaacaa
    28141 gctggtgatg tattcaaaga tccagaaacg atcaatggaa aaacaatcat tgcagcacaa
    28201 gtaaaagtga aagatatgaa tcagctgcgt caattagctg accaatggaa acaaaaaaat
    28261 atttcagata ttcttgtttt agcaaacgaa caagaaggaa aagtcagtct gctagcggca
    28321 atgacgaaag aatcaaatga agctggacta aaagctggcg acttgattaa agcaatcgca
    28381 ccgaaagtag gcggtggcgg tggtggacgt ccagacatgg cacaagctgg cgggaaaaat
    28441 cctgctggta ttccagatgc acttaaagct gcgaaagaat gggttgcgca acaatagggc
    28501 gtttacgttt gacagaaaaa caaaagttca aacattgaac gaaacatgat tatgggggcg
    28561 aagacaaaca agtcttgccc tcatactttt ttcaattgga tggcgcatct aatcaaatgg
    28621 tcggattcgg atggtcttta gttgacgaaa gtgacagaaa tttttacgat tagtataaat
    28681 aaaaaggggt gaaaaaatga atagtagaaa taagaccttt cgactagtct tacgtgcgat
    28741 cttgttagca atcatcatcg tacaagcgat ggttccttgg ctggggttta ttccattagg
    28801 attcattagt ttaacaatta tccatatcac agtaattatt gctgcagtag tattaggccc
    28861 aaaagatggc atggtgattg gcttgttttg gggaatcgct acaattgttc gtgcctatgc
    28921 catgccaaca acgccatttg atacattagt gttcacgaat ccgattatct cggtagttcc
    28981 cagagtactt gttggcttag tagcaggctt ggttttccac tggatttatc aaaaaaataa
    29041 atcaataacg atcagctcag cctttgcagg ggtactgggt tcattggtca atacagtctt
    29101 agtgttagga ttcatgggat tattttatac aggtgcaacg gcagatgcct atggtgtaga
    29161 ctctgctctt ttatttaaaa ccttagcagg ggttgcagca atcaatggga taccagaagc
    29221 aataggtgcg ggaatcataa caccattact agcaaaagcg ttatttgcag caacaccgtt
    29281 gaaaccagaa taatgaccat cttttaatag taataggacg cacgatagat agaaaaagga
    29341 agagaggaga ggaaaacaat cgtcctctcc atctttttac ctgagcaaca agcaaactag
    29401 aaggggtaaa aaacttggct aagaaacaag aagaaaaaaa gaaacaaata ccgcaacagc
    29461 ggacaaaaaa aggagtacta gcgggtatct taggattggt tacgaatctg atcttgtttg
    29521 tctctaagct aatgatcggg cttttttcag gaagtgtctc cattatggct gatgcagtga
    29581 acagtctatc agatactgct tcatccatat tgactttgat cggatttaaa attgctgcaa
    29641 agccagcaga tcaggaacat ccttatggac acgaacgctt cgaatatatc agtgggttat
    29701 ttgtttcgat catcattact tatgtaggat ttcaattttt agagtcttct gctgaaaaaa
    29761 tcatccatcc agaaaatgtc tctttaacac ccatcgtctt ttttgtccta atcatttcca
    29821 tctttttgaa atttgcacaa ggcaaaatgt atcaggttat tgcgcaatcc attgattctg
    29881 aaacattgag agctacagca acagatagtt ataatgatgt ctttactaca ctggctgtat
    29941 tgctttcagc agcaatcgaa tgggtaactg gatggcgtat tgatggatat ataggttttg
    30001 ctttagcatt atatatcctt tatagtggat tgacgatgct tcgaaatttt gtatacgaac
    30061 tattaggaag tcgcccaacg ccagcagaga ttgaagaaat ggaagagcat ttaaatgctt
    30121 gcgatgatat acttggctat catgatttat tggtgcataa ctatggaccc aataaacgct
    30181 ttgcatcagt ccacatcgaa gtagatgaat caatgaattt gaatgtggct cataagatta
    30241 ttgatctgat agaaaaagac tttaaaaagt ctttggatgt ggaacttgtc tgtcacttgg
    30301 atccaatgcc tgttgataac gaaaaatact ataatatctt gcggcagtta aagcaaattc
    30361 ttcttgaaac aggtacaggg cttaatattc atgattttag agtaattcat caaggaaaaa
    30421 cgctgcaatt tgatgtagtg gtccctgaga aattccagtt tgttgataca gaactagaaa
    30481 gaatcttacg tagtcaaata gaagaaaaaa tcggagatta ccaattggaa attacgtttg
    30541 atcacaatta tcttttatat taaatgagaa actataaaaa gactccttaa agtttttaat
    30601 ttaaagagtc tttttttctt ttaattgttt atggagagcc tgacgtgcaa gattatctgc
    30661 tcctttattt ttgttttcgg gcaaccattg caggatcaat aaagaaaatt cggatagaag
    30721 ttcttggatt ctttgaaggt agggaaggaa gagctcgttc ttcgttgcgt ttttgtcgac
    30781 cgtagaaact agtgttttgc tgtctgaaaa acagaaaatc gtttcattct gtaagtcgtt
    30841 tttctttaga aattctaaag tttttaaaaa gacttcgaac tctgcttgat ggtttgtccc
    30901 tatagaaagc gggatagata gctgttcttg tttaccttga tagacaatca agattccacc
    30961 tccactagga ccagggttgc cttttgtaga ggcatcaatg tatactttta acataaaaag
    31021 agattctcct ttttgttcaa actaatgtta tggtaagatt atatcattac tgaatcggtg
    31081 agaggttaga gaatgaaaaa aaatattcga tggcagcctg aactggcaca tacgatcatc
    31141 tattggtcat tgaccttgat cattctgttt tttagtctga ttctttcttt agaaaacaca
    31201 ggtccttatt ggaaaagcaa ttttgtttta ggtatttttc ttgtttttct atttctaggt
    31261 ttgcggcgtt cttttatttt aaaatcaaac tctgtgatta tacgttatgc tgctttttgg
    31321 aaagaccgag aaatatctgt cggacagatt cgatctttga cgccaactaa tactggggtg
    31381 caaatgatgt tgcaagatga gaaagcccca tctgttttca tgatgcgaaa aaaagatcaa
    31441 gtagtccttt ttgatcattt tgaaaaaaac ggacccaaat cagaatcaat tccttatcct
    31501 tatacgaaaa aaagaaaaag ctaattttaa aaatcagaac ctaaaaaaac gaatttttac
    31561 aggaaaagaa aagagtgaga cgtacggttc atcccgttta gcgtctcact ctatttgttt
    31621 atctattcat gcatactagt atcggtagtc tgaacgttgc ctttcttgac atgggcagct
    31681 tgtgttcctc gattcccttc gatgatctca aattcgacca cttgattttc ttccaaagtt
    31741 ttgaatccct cttcttcaat cgcagtaaaa tgtacaaaaa tttcttctgt gtcatcataa
    31801 ctgataaagc cgtatccttt tttgttgtca aaccatttta caattcctgt ggtcaaagca
    31861 ctcacgacct ttctaaagtc tgatcatagc cttcattata aagagtattt ataagtacgt
    31921 caaataaatt aaaaacctgt aattttatgt tttttttctg aataaaaaaa ggtataattg
    31981 gcatatctta acaaagaaga tattttgttg aataaagttc gtgaaaaggg gtaatcaaat
    32041 gaagaatgat ctgcaaattt cacaagaaac agagctagaa ccgattgttg aagtggctag
    32101 taaattagat cttaaggaag acgatttgga actgtatggg aaatataagg cgaaaatcaa
    32161 tttttctgga atcaatcgat caaaggataa cgctgagggg catttgatct tagttacttc
    32221 catcaatcca acaccagctg gcgaaggaaa gtcaacgatc actgtagggt taggagacgc
    32281 gctcaataaa atcaataaaa aatctgttat tgcattgcga gaaccctcac ttggtcctgt
    32341 gatgggaatc aaaggtggtg ctgcaggcgg aggatatgca caggtattgc ctatggaaga
    32401 tatcaacttg cattttactg gtgacatgca tgctatcaca acagctaata atgcattatc
    32461 tgctctttta gataatcata ttcatcaagg aaatgaattg aacatcgatg cccgccgagt
    32521 gatttggaaa cgggtagtcg atctcaatga tcgtgaactg cgtaaagtaa ttgttggctt
    32581 gggtgggcca atccaaggtg ttccaagaga agatgggttt gatatcacag ttgccagcga
    32641 aatcatggct atcttgtgtc ttgcggcaga ccttgaagat ttaaaggctc gtcttgcaag
    32701 gatcgttgtc ggctatacat ttgaccgtca gcctgtgact gctggagact tgaaagcaga
    32761 aggcgctctg gcattactgc tgaaagatgc aatcaagccg aatcttgtac aaacaattta
    32821 cggaacacca gcttttgtcc acgggggacc gtttgccaat atcgctcatg gctgcaatag
    32881 tattttagct accaagacag cattgagatt agcagattac gttgtcacag aggcaggatt
    32941 tggtgcagat cttggcggag agaaattttt agatatcaaa gtacctaact tgaaaaaagc
    33001 gccggatgct gtggtgattg tcgccactgt ccgtgcattg aaaatgcatg gcggaatgaa
    33061 aaaagatgaa cttaaaaatg agaatcttga tgctttgaaa gtaggttttg caaatttaaa
    33121 gcgccatata cgtaatatgg aacaatatca gctacctgtg atcgtcgcaa tcaatgaatt
    33181 cgtaacagat acagacaatg aactcacact gttggagcac ttgtgtgaaa atcaggggat
    33241 tcttgccaag agagcgagtg tatgggcaga tggagcagaa ggcggcgttg atttagcaaa
    33301 agctgtagta cgattgattg atagaaaaga agctaattac aagccattat accgcctaga
    33361 agaaacgata caagaaaaga cagaaacaat cgtcaagaaa atttacggag gaaatggtgt
    33421 tgttttcagc aaaaaagcgc aaaaacaaat agaagagttt acgaagaatg gatgggacaa
    33481 actgccgatt tgtatggcaa aaacacagta ttcattttct gatgatcctt cactgctagg
    33541 tgcaccggaa gatttcacca tcacgatccg cgaaatcatc ccaaaattag gggcaggatt
    33601 tttagtagcc ttgactggag atgttatgac gatgcctggt ttgcctaaaa aacctgctgc
    33661 attgaatatg gacgtgacaa atgacggaga agtgttggga ttgttttaag cttgattata
    33721 atctaccatc gaaggagctg tattttgact acagcttctt tttttagacg atagggaatt
    33781 aattttgttt acactaggat cattcttttt ctttattgta ttttgagcta tactaaagca
    33841 taataggagg gtatgatgaa aacgacattt ataagaaaaa ccggttttta cggtatgggt
    33901 agctcgattc agctaagaat caacaatgaa aaacgcagtg ttctcagtca tgaacaaaag
    33961 ataacgcttg atctggaagt gccctttacg atgcaggtca ccttttattg gctaaaaagc
    34021 cccgtttata cagtgaaaga agcaaagcct gcttatgtga tcacaatgaa ttacttgatt
    34081 cttcaaatct atccgttttt atttctagca actgggttgg gggccgtttt cctacaaaat
    34141 ttatttttca gtttttttgc agttattgtg ttgattgcgt tctttttcta tataaaaaac
    34201 cgggcatatt tgatcaagga ggaaaattat ggcaaactct gatctttttt taatcagttt
    34261 caatcgaatc gaaaaatgga tgagagacga gatgggaaat gcacgaaata tgggatttac
    34321 cgaacttgtc agacgcctag ctcaaagaaa acaattaatg atcagaaaat acgaagatga
    34381 tcttttacag cttgctcagt taaggaatgc gatcgtccat gatcggatcg cagtcgattt
    34441 tgtcattgca gagccaaatg attggacagt caagcgaatc cagctgatcg aacaagaact
    34501 gattcagcct gaaaccgtgc tgcctcgatt tgcaaaacgt gtaacaggtt ttgaacaaga
    34561 tcttccgtta cttgaactgt taaagatagt agcagaaaaa aggtattcac aatttccgtt
    34621 atacaataaa ggaaaatttg tagcattgat tactttacgt aatatcgggt tttggctagc
    34681 aaaagaaagc caaaaaggtc cggttgattt aacaaataaa cgagcgttgg atttgatcat
    34741 ccaaaacgga aagtacacaa attatcattt tgttccagct aaaacacata tttacgaggt
    34801 tgaagcgatg tttcgtgagc aagggacttt ggaagccgta cttatcacta aagatgggaa
    34861 tcccgatggt aatctgctcg ggatcgtacg acctcgtgat atttataaac aaatagaaaa
    34921 ggattgattt tttgttaatt atttattgga tattcagcgc tttgctagta ggagtggatc
    34981 agttggtcaa atggtggatc actgataatc tagcgcttgg agagacgaaa aatcttatac
    35041 caggtatttt gtcactgaat catattcgaa acactggagc cgcatggagt atgctagaag
    35101 ggaagatgtg gttctttact atagtgacac tcattgcagt cgttgtgatt ctcacattga
    35161 tgattaaaaa tcgtgaaaaa ggtaatcgct ggttcatgat tggattgagt ttgattctag
    35221 ctggtgcgat tggcaatttt atcgaccgta ttcgtcttgg ctatgtagta gatatgttcc
    35281 agacagattt tatgagcttt ccgattttta acgtagcaga tgtgacactt gtatgtggag
    35341 tgatttgtat gttaatttat attattttag atgaaaagga tcaaagaaaa aaatgacaga
    35401 acttaaaacg acgattcacc atgaatctgg taggatcgat aaagtattga ctaatttatt
    35461 caccgattac agtcgttcgc aaattcagct gtggctgaaa aatggagcgg tttctgtcaa
    35521 tggacagcca gtcaaagcaa attacaaagt aaaacaagga gacgagatta ctgtcttgat
    35581 ccccgaacca gaaacattag atgtggtccc agaaaatatc ccgttgacga tcgtttacga
    35641 agatgaagat gtagcggtga taaacaaacc tcaaggaatg gtcgttcatc cttcagcagg
    35701 tcatacgcat ggcacaatgg tcaatgcgct gatgtaccat atcaaggatc tttctacgat
    35761 caacggcgtt attcgtccag gtattgttca ccgaatcgac aaagatactt ccggactgct
    35821 aatggttgcc aaaaatgacc aggcacacga atcattagct cagcaattaa aagataagac
    35881 ctctttgcgc aaatatgttg ctttagtcca tggagagatt cctcacgaaa aaggaaccat
    35941 caatgcgcca atcggccgtt cgaaggaaaa ccggaaaatg caggcgataa gagaagatgg
    36001 gaagtcggct gtcacacatt ttactgtatt ggaacgtttc gaaggattta ctctggttga
    36061 attaaaacta gaaaccggaa gaacacacca gatccgtgtg catatgaagt atatcggtta
    36121 tccgttagct ggagatccag tatacggacc gaagaaaact ttgaaaggca atggtcagtt
    36181 tctccatgcg caaatgttag gttttacgca cccgactaca aacgaaaaga tgatctttga
    36241 agcacctctt ccagaagttt ttgaaaaaac tttagaaaaa ttaagaaaaa acattgcatt
    36301 ttaatttaga taacggtata gtaattaaca acgaaagcat aaaacttaat agaattcctt
    36361 taagtttagt cccgtgaggc taagaagggt gggagcaagg atacgtatgg tgcgccataa
    36421 gtagattgct cattgacata gtatagctat cagtgtatca gtatacgctc agtctaaaca
    36481 ttcctcctgt cctcttaggg caggagtttt tttatgcaaa aaagtagggg gaatgaaaat
    36541 gcaagcaaaa gaagtagtag atcaggtaac catgaagcga gcattgactc gaattactta
    36601 tgagattatc gagagaaacc atagtatcca agatatcgta ttggtaggta tcaaaacacg
    36661 aggaatctac attgcatcaa gaattgccga acgcctgaag cagctagaag atatcgatat
    36721 tcctgtagga gaactggata tcactttata ccgagatgac aaaaaagaaa atccagaaga
    36781 accggaactt cattcttctg acatcccagt ttccttagaa ggaaaagaag ttattttgat
    36841 tgatgatgtt ttgtatacag gacgaacgat tcgagcagca atggacgcag taatggattt
    36901 tggcagacca agaaaaattt cattagcagt cctggtcgat cgtgggcatc gagaattgcc
    36961 aatccgtgca gattatgttg ggaagaacat tccaacatct cgggcggaag agattttagt
    37021 agagatgcaa gagcttgatg gacaagatcg catcatgatt ttgaaagagg aagattagag
    37081 gagagagaaa catgtcggag aaaaaaggat ttcataatga tgacgtggtg ttagatatcc
    37141 acgatcgccc aaccccagga cattgggtag gattaagttt acaacacttg ttcacgatgt
    37201 tcggagcaac cgtgttagtc cccatcttag taggtatcga tccaggtatt gcgctagtca
    37261 gttcaggact aggtacattg gtgtacttat ttactactaa aggaaagata cctgcgtact
    37321 taggaagcag cttcgctttc attgcagcga tgcagctttt gatgaaaaca gatggttatc
    37381 cagcgattgc acaaggagca gtcacaactg gattagtcta tttgattgtt gcgctgatca
    37441 tcaaaaagat cggttcagca tggctggata aaatcttgcc tccaatcgtc gtcggtccag
    37501 tcatcatggt tatcgggttg ggacttgcgg caaatgcagc aacaaatgct atgtataatc
    37561 agggcacgta tgatttcaag tatattgctg tagcgctgat cacattagga ttaacaattt
    37621 tcttcaacat gtggctaaga ggttttctcg gattgattcc gattttacta ggtattatta
    37681 gcggatacat cgtagccttg ctgtttggaa tcgtggatac taagccaatc gtagacgctg
    37741 cctggtttgc tgttcctaac tttgagattc cctttgtaca atatgaacca aagctatatc
    37801 ttggagcaat cacaacaatg gcacccatcg cttttgtgac gatgacagag catatcggcc
    37861 atcttatggt attgaataaa ctaactaaac gtaatttctt tgaagaaccg ggtcttcata
    37921 agacgttgac aggtgatgga ttagcacaaa tcgtggcagc ctttgtcgga ggtcctcctg
    37981 taacaagtta cggtgaaaac attggtgttc tggctatcac acgtgtccat agcgtgtttg
    38041 tgatcggtgg agctgcagta tttgcagtca ttttaggatt cgtaggaaaa ttaagcgctt
    38101 tgattttaag tatccctgga ccagtcatct caggaatcag ttttatctta tttggagtaa
    38161 ttgccgcaag tggaatgaag attttaatcg agaacaaaat cgattttgat aaaaagaaaa
    38221 atctgttgat tgcttcagtt atcttagtag taggaattgg cggactcgtc tttgaagtag
    38281 gcacatttac attgtcggca atggcattag caactgtact tggcatcatt ttaaatctta
    38341 tcttaccaga aacttcacga agcgaagaaa actaaggagg aaacattatg atcatcaaat
    38401 cagaacgtat cagcttaaag catttattga ccgttgaggc acttactgat caagaagtta
    38461 tgggcttgat ccgcagaggg caggaattca agcaaggtgc taattggact ccgcaaaagc
    38521 gccagtactt tgctacaaac ctctttttcg aaaatagtac tcgcacacat aaaagcttcg
    38581 atgtagcgga gaaaaaacta gggctagaag tgattgaatt tgaagcaagc acaagctctg
    38641 tccaaaaagg ggaaacacta tacgatacag tactgacgat gtcagcatta ggcgtagacg
    38701 tagcggttat ccgtcatggt gatgagaact attatgacga actgatacaa agtaaaacaa
    38761 tccagtgttc gattatcaat ggaggagacg gaagcggaca gcatccaacc caatgcttgc
    38821 ttgatttgat gaccatttat gaagaatttg gtcattttga aggtctgaaa gttgccatcg
    38881 taggtgatat cactcactct cgcgtagcta aatccaatat gcagatgtta aaacgtctag
    38941 gcgcacaagt attcttctca ggtcctagag aatggtacga cgatgaattc gaagtgtacg
    39001 gacattatat gccattggat gaactactag atcaagttga tgtcatgatg atgcttcggg
    39061 tgcaacacga acgccatgat ggaaaagaaa gtttctcaaa agaaggctac catcaggaac
    39121 atgggttaac agttgagcgt gcgaagaagt tgcaaaaaca tgcaatcatt atgcacccag
    39181 caccagttaa ccgtgatgta gaattagctg attcattagt ggaaggatta cagtcgcgga
    39241 tcgttcagca aatgagcaat ggagtattcg tcagaatggc aatcttagaa gcagtgctaa
    39301 atggaaaagc gtaattatag gaggatgaaa aaatgaaaac actcattaaa aacggtcaga
    39361 tcaatacacg aaaaaatatg acgacaccag cagaaatttg gatagaagat gggcgaatca
    39421 aagcaatcgg tacaggattc tcagaagccg aatttgatga agtgtttgat gcaaagggac
    39481 aattgatcac acctggttta gtggacgtcc atgttcattt gagagagcca ggatttacat
    39541 acaaggagac gatcgaagct gggactcgct cagctgctcg tggcggtttc actactgtat
    39601 gcgcgatgcc gaatttaaat cctgtacctg atacggcaga aaagctgaga caagtatatg
    39661 acatcattag aaaagatgca gttgtgaagg tattgcagta tgcgccaatc acagaaaatc
    39721 tgcgaagtga gaaattagtt gatcaagaag cactgattga agaaggtgct tttgctttca
    39781 caaatgacgg tgtaggtgtc caaacagctg ggacgatgta tctggctatg aaagaagctg
    39841 caaaaaacaa taaagcccta gtagcccata cagaagatga atctttatta tttggcggcg
    39901 ttatgcacgc tggcaagaag gcagaagaac ttggtcttcc cgggatctta agcgtaaccg
    39961 agtcttctca aattgctaga gatctgttgc tagctgaagc aactggtgtt cattaccatg
    40021 tttgccatgt ttctactaaa gaaagtgtcc gtgtaatcag agacgcaaaa aaagcaggaa
    40081 tccacgtaac agcagaagtt tcaccccatc atcttatttt gatcgacgaa gatattcccg
    40141 aagatttcgg cttttggaaa atgaacccgc cgctaagagg acgggaggac agagaagctt
    40201 tgatcgaagg cttgcttgac ggtacgatcg attgcatcgc tacagaccat gccccccatg
    40261 gattagaaga aaaaagccaa agtttcatga aatcaccatt tggtatcgta ggaagcgaaa
    40321 cagcgtttca actgatctat actcattttg tagaaactgg tcgattcacg ttggaacaag
    40381 tgatcaattg gttggcagtt aaaccggctg agatttttgg tttgaatgca ggaacgctaa
    40441 cggttggtgc ccccgctgat gtagcagttt ttgatattac acaaacttgc acgatcgata
    40501 aagaagactt tttatcaaaa ggggagaata caccttttat cggctggaaa gtaaaaggcg
    40561 aaacacaaat gacttttgtc aacggaaaac ttgtgtggca gaagggagaa taaaagtatg
    40621 gaacgtttgc ttatattaga agatggaaca gttttcgaag gaaaggcttt tggtgcagat
    40681 gctagtgtct tcggagaaat cgtttttaca acaagtatga ctggttatca agaaactatc
    40741 actgatcaaa gttttaacgg tcaaatcatt acatttacct atcctatggt gggtaattat
    40801 ggggtaaata gagatgatta tgaatcgatt tctccaacat gcaaaggagt agtagtaaaa
    40861 gaacatgcac gtgtagcttc aaactggcga aaccaaatga cgctagattc atttttgaaa
    40921 caaaaaggaa ttcctggaat atcaggtatc gatacgcgcg cccttactcg caaaatccgt
    40981 gaagtcggaa cgatgaaagc tagtatagtt gatgctggag attccttcga acatgcattt
    41041 gatcagctga aagcaaccgt tctagcaacc aatcaagtgc agcaagtctc aactaacaaa
    41101 ccttatccaa gtccaggaac aggtcggaaa gtcattgtca ttgacttcgg cttgaaacat
    41161 agtattttac gagaattgag taaaagaaac tgtaacctca cagtacttcc ctataacaca
    41221 gatgccgaga cgattttagc gctatcacca gacggagtca tgctgacgaa tggacctggt
    41281 gatccaaaag atgtgccaga agcaatcgaa atgatcaaaa aaatccaagg aaaagtaccg
    41341 atttttggta tttgtctcgg tcaccagtta tttgctttag ccaacggtgc agatacctat
    41401 aaaatgaaat tcggacatcg aggattaaat cacccggtac gagaagtagc gaccggaaga
    41461 attgatttta cttctcaaaa tcatggctat gcagtcgatg aagccacgat tgatccagaa
    41521 aaactcatcg taactcatgt agaagtaaat gacggaacag tagaaggagt caaacacaga
    41581 gattatcccg ctttcagtgt gcagtttcat ccagatgctg cccctggtcc gcatgatgca
    41641 ctacatttat ttgatgaatt tatggaaatg atggacgcag gaaaggagca gtaaacagat
    41701 gccgaaacgt acagatatca agaaaattat ggtgattgga tctggtccga tcatcattgg
    41761 tcaagccgca gaatttgact acgcaggaac acaagcctgc cttgccttaa aagaagaagg
    41821 atatgaagtg gttttagtaa attctaatcc tgcgacgatc atgacggata aagaagtggc
    41881 agatcaagta tatatcgaac cgatcacact tgaatttgta tcaaggattc ttcgaaaaga
    41941 gcgtccagat gctctccttc caactttagg cggacagaca ggcctcaata tggcaatgga
    42001 gctggcagca tcaggtattt tagaagaatt gaacatagaa cttctaggaa caaagctttc
    42061 tgcgatcgat caagcagaag atcgtgatct attcaaacag ttgatgaaag aattgaatca
    42121 gccaatcccc gagtctgaaa tcgtcaatac agtagaagaa gctgtagatt ttgcaaataa
    42181 aatcggctat cctgtcatcg ttcgtccagc ctttacttta ggcggaactg gtggaggtat
    42241 gtgtgaaaat gaagaagaat tacgtatcgt atcagaaaat ggattaagtc tttcccccgc
    42301 tacccaatgt ttgattgaac gaagtattgc aggttttaaa gaaattgaat atgaagtgat
    42361 gagagattcg gctgataatg caatcgttgt ctgcaatatg gaaaattttg acccggtggg
    42421 tatccataca ggtgattcta tcgtatttgc ccctagtcaa acattgtctg accatgaata
    42481 tcaaatgctt cgtgatgctt ctctttcgat tattcgagca cttaagatcg agggaggctg
    42541 taatgtccaa ctcgctcttg atccacatag ttttaattat tatgtgattg aagtcaatcc
    42601 gcgtgtttct cgttcatctg cgttagcaag taaggcgact ggctatccga tcgctaagct
    42661 agctgctaaa atagcgcttg gattaacatt agacgaaatg aaaaatcctg ttacaggaac
    42721 gacttttgca gaatttgaac ctgcattaga ttatgttgta gctaaaattc ctcgctggcc
    42781 gtttgataag tttgaaaacg gagaacggct tttaggcacg cagatgaaag caactggtga
    42841 agtgatggct atcggtcgga atatcgaaga gtctttattg aaagctgtgc gttctttaga
    42901 aatcggtaca caccatatcg aacttcctga gttggcagac atcacggacg atgacctaat
    42961 ccaaaaagtg gttcgagcac aagatgatcg cctgttttat gtagctgaag ctattagaag
    43021 agggtacacc atagaagaat tagcggattt gacaaaaatc gatttattct ttttagataa
    43081 actgttgcat atcgtagaaa tcgaacagca gttgaaacag cagccgttgg atgacaagat
    43141 attgaaaaca gcgaaacaaa acggttttac agacaaaaaa gtggcggagc tgtgggaaat
    43201 aaacgaagaa gccgttcgcc agatgagaaa agaaaatgat ttgattcctg tatataagat
    43261 ggttgacact tgtgcagcgg aattcgaatc aaaaacacct tatttttata gttcctatga
    43321 atatgaaaat gaaagttcac gatcagaaaa gccctcaatt ctagtactgg gatctggtcc
    43381 tatccgtatc ggacaagggg ttgaatttga ctatgcgact gtgcattctg tcaaagcaat
    43441 ccagcaggca ggatacgaag caatcatcat gaacagcaat cctgaaacag tttcgactga
    43501 cttttctatt tcggataaat tatattttga gccattgacc ttcgaagatg tgatgaatgt
    43561 gatcgagcta gaacagccga taggagtaat cgttcaattt ggtggtcaga cagcaatcaa
    43621 cttagccgaa cctttgactg ctgctggtgt aaaaatctta gggacaacga tcgaagactt
    43681 ggatcgtgcg gaaaatcgtg atttattcga gcaagcgctg aaagagttgg atattcctca
    43741 acctttaggg gatactgcta caagtaaaga agaagccgtg gttatcgctt cgagaattgg
    43801 ttatccagtt cttgttcgtc caagctatgt attaggcgga cgtgcgatgg aaatcgttga
    43861 aaaccagcga gatttagaag attacatgga acatgctgtg aaagcatctc cagaacatcc
    43921 agtactagtg gaccgttact tgcttggtgg tgaatgcgaa gtggatgcga tttgtgacgg
    43981 tgaaacagta ctcatcccag ggatcatgga gcatattgag cgtgctggtg tccattcagg
    44041 ggattcaatg gctgtgtatc cacctcaaac attatctgaa tcgatcaaga aaacgatcga
    44101 agattacacg atccgcttag cattaggttt gaattgtgtc gggatgatga atatccagtt
    44161 tgtcatccat gaaaatgagg tttatgtgat cgaagtcaat cctcgagcaa gtcggactgt
    44221 tccattctta agtaaggtga caggaattcc aatggcacaa attgcgacga aagctatttt
    44281 gggagagaaa ctaaagacac ttggttatga aaacggactt tatccagaaa caaaagaagt
    44341 tcatgttaaa gcacctgtct tttcttttac gaaattgcag aaagttgata cttacttagg
    44401 gcctgaaatg aaatcgactg gagaagtaat gggatctgac agacaattag acaaagcttt
    44461 gtacaaggca ttcgaagctt ctggattaca cttgcctgaa tatggagccg tattatttac
    44521 gattgcggac gaaacaaaag aagaagcact agaattagct aaacgtttta cagaaattgg
    44581 ctatagtctg ataggaacga aacatacagc tgcttatttc gaaaaaaatg gtctggtcgt
    44641 tgaaacagta gcgaaaattt ctgaggaagc agcagaaaaa aatgtagtcg acttaataag
    44701 agatggtaaa acacaagtgg ttgtcaatac gattgataaa gatcggggaa atgcttctaa
    44761 agacggtttc atcatccgaa gagaagcagt tgaacatggt attccattgt ttacttcttt
    44821 agacacagca gatgcgatcg ttcgagtgat ggaatcccgt gctttttcaa ctaaccctat
    44881 ttaacggcga gcacatgtta agacaaaaag acgaaaaacc gttgcaggag gggacagatg
    44941 aaacaagaaa tgatggaaat cgttcagcaa aaacagctgg caccaaagat ttttgaaatg
    45001 acattgaaag gtgatctagt aaaagaaatg cagcaaccag gacagttctt gcatattctc
    45061 gtgccgcgtg cagacttgct attacgccga ccgattagct tgaatcatat cgaccacgaa
    45121 acaaatactt gccgtatcat ttatcgggtg gaaggtgagg ggactagagt cttctctact
    45181 ctttctgccg gagaccattt agatgtcatg ggtcctttag gcaatggttt tgatttgtcc
    45241 aatcttcata caggagacga agtatttata atcggtggag gaatcggtgt ccctcctcta
    45301 tatgagttat caaaacagtt agtcgaaaaa ggaattaagc caacgcattt tttaggtttt
    45361 gccacacatg aagtcgttta ttatgaaaaa gaatttaatc gtctaggaga aacaagaatc
    45421 gctactgacg atggaacata cggtatgcac ggaaatgtcg gtaatttatt gttaggaacg
    45481 aagacggaac ctacagctgt ttttgcatgt ggttcaaacg ggttattgaa gacagtggaa
    45541 cagctgtttt cttcgcatca aaatgtccag ttatcattag aatcccgcat ggcatgtggg
    45601 ataggggctt gctatgcttg cgtttgtcat acgccggaag atgaaacagg aacaaaaagt
    45661 gtgaaagttt gtgacgaagg accgattttt aaaataggag aggtaattat atgaatgaga
    45721 atcctttagc ggtcaaactt cctggcctag aattaaaaaa tcctatcatg ccggcaagcg
    45781 gctgtttcgg ttttggcaaa gaatacggaa aatactatga tttgaatcag cttggatcga
    45841 tcatggtcaa ggcaacgact ccaaatgccc gctttggcaa tcccacgcca agagtggcgg
    45901 aaacaccatc tggcatgttg aatgcaattg gtttgcaaaa tcctggtcta gacgttgtta
    45961 tgcataaact tcttccagaa ttagaggagt acaatgagtt acccatcatc gcaaatgttg
    46021 ctggtgcttg tgaagaagac tatgtagaag tttgtagcaa aattggcgaa gcaccaaatg
    46081 tgaaagcaat cgagctgaac atttcttgcc caaatgtcaa acatggcgga attgcttttg
    46141 gaacagattc tgatgttgct tttcaattaa cacaagcagt aaagaaagtt tcttctgttc
    46201 ctgtctatgt caaattgtcc ccaaatgtaa cagacatcgt tccaatcgct caggcaatcg
    46261 aagctggagg ggcagatggg tttacgatga tcaatactct tcttggtatg cggattgacc
    46321 ttaagacaag aaaaccgatt ttagccaatc aaacaggtgg actctccgga ccagcaatca
    46381 agcctgtcgc tattcgtttg attcaccaag tagcaagtat atccagcctt ccaatcattg
    46441 ggatgggcgg cgtgcagaca gtggatgatg ttcttgagat gtttatggct ggagcaagtg
    46501 ctgtagctat agggaccgcg aatttcaccg atccgtatat ctgtcctaaa ctaattaaag
    46561 agctccctgt gcgaatgagc gaactaggca tcgaatcgct ggaaagatta atcaaagaag
    46621 tgagagagga acgtgaacga tgaatcaact agaccaacga ccaattattg cattagattt
    46681 ttcaactagg caagaggtag aagactttct tcgatttttc ccgaaagaag aaaaattgtt
    46741 tgtgaaaatc ggaatggaat tgttctacca agaaggacct gaaatcgtac gttatctaaa
    46801 agaggctggt cataatgtat ttttagatct gaaacttcac gatattccga atacggtcga
    46861 aaaagcaatg cggggattgg caaagcttgg tgttgatctc acctgtgttc atgccgctgg
    46921 cggtattcgt atgatggagg cggcaatgcg aggattagaa gaggggactc cagaaggtgg
    46981 aaaacgtccg ctacttctag ctatcactca gcttacttct acaagtgaag aagaaatgca
    47041 tgccgatcaa ctaatcgaag tttctttaga aaaaagtgtg atccattatg ctagctgtgc
    47101 caaaaaagca ggattagatg gtgtggtttc ttctgcttgg gaagtagaag cgatcaaaga
    47161 aatggcagga gacgaatttg tttgcttaac tccgggtata agaccagaag gaacagtcgc
    47221 aggagatcag acaagagtag tcacaccttc tcaggcaaaa aagatcggct caacttttat
    47281 cgttataggt cgaccaatca ctcagtcaac taatccttat aaagcatatc aaacgattca
    47341 aacagaatgg agctaaccta aaatgaatgt tgaacaaagt atcgcaaaag acttattaga
    47401 aattgaagca gtatttttaa atccaagtaa tccttttaca tgggcaagtg gaatcaaaag
    47461 cccaatttac tgtgataacc gaatcacgat gagctatcca aaagtccgaa aagagatcgc
    47521 aaaaggatta gcaagcaaaa tcaaagaagc ttaccctgaa gtacaagtga ttgctggaac
    47581 tgcaacagca ggaatccctc atgccgcatg ggtagcggaa atactggatt tgccaatggt
    47641 ctatattcga agtaaggcga aagatcatgg gaaaggtaac caaatcgaag gacgtatatt
    47701 tgaaggtcag aagatggttg tgatcgagga tttgatttct acaggcggaa gtgtcttgga
    47761 agcagctgaa gcagcaaaaa gagaaggtgc ggatatcctt ggtgttgcgg ctatctttac
    47821 gtatgagttg ccaaaaggaa aagcaaattt cgaaaaagcc gaaatccctc ttatgacgtt
    47881 gaccaattat tctgtattga ttgaagcagc tttagaagat cgttatatcg atgagcaaga
    47941 attaactctg ctaaaagaat ggaaaaaaga tcctgaaaat tggcagattt aagcgcgctt
    48001 gatgaagata gagaaatccg atttgtgtct ccagaatcat caattggaat agcaaaaatc
    48061 caaaaactga ttgcgagaag catcttttca tcaggtatga aaagaaactt ctatcgtgat
    48121 cagtttttga tttttttaaa attcattttt ctaagactgg ttagcagaaa gaagagaata
    48181 atctcttctc ttttttctat tatatataga ttttctctat tttttggtaa gggagctgtt
    48241 tcaaaaatgg taaagagcgg aaatgttgcg aatatcagtt atttaatgat atgatattcg
    48301 gaaaaagaaa tctcaaaggg aggtaacttt tatggggttg aatgaattta ttttaggatt
    48361 gaacaattta gagacgatca caagggcacc tggatttttt aaatatacag agcatacagt
    48421 tgcagcacat tcgtatcgtg tagcatctat tgcgcaagtt ctaggagaca ttgaagaagc
    48481 gaatggacaa ccgatcgatt ggaaactgct ctatgaaaaa tcattgaatc atgactacac
    48541 agagcgtttt attggtgata tcaaaacgcc ggtgaaatac gcaagcaaag agttaagagg
    48601 gatgctgcaa acagttgaag aaaagatgac ggacgaattc atcaaccaag agattccagc
    48661 tgagttccaa gaagtttatc gccgtcggtt gttcgaagga aaagatgaaa ccgtagaagg
    48721 agaactatta gccatttcag ataaggttga ccttctttat gaatcttttg atgaaatcat
    48781 caaaaataac ccggaaaaag tttataagga gatgttcatt gaggcagtga ctacgattaa
    48841 aagctttgaa catcggcctt ctgttcagta tttctttaaa gagatatttc cggaactatt
    48901 gaaccaggaa ttttatggaa aagaggcgtt tttagaaacc gtctcaacga ttgtaaagaa
    48961 agaagaaaaa taaaaatgat atgagatgca gggggaagta tagttgaaaa agaatatgga
    49021 tgtcgaatgg ggctattctg gagacaaagg tccggaaaat tgggcgagtt tatgtgattg
    49081 gtttgcgaga ggtgcggaat ttccattgca gtcgcctatt catttgacca agggggaaga
    49141 aaccaaaata gaaggagaca cgctttcctt tcattatcaa aagcaacggt ttactgacaa
    49201 ggaattcaaa aacacgattc atttagtacc ttttgaccaa ctcagttatg tagagttcaa
    49261 gaaagaacgc tattatttga cagatattca tttccacttg ccttctgaac atatcatcaa
    49321 cggaaaacag gaagatattg aatttcattt tgttcatatg aacagcaaaa aagagaatct
    49381 agttgtgggt gtcatgtacc agctaatggt acaggaaggt tggctgtata atcaagaaaa
    49441 tggggaaagt tggaacttag aaaaacatga gcattgggta aatccggatg tgttctttcc
    49501 ggagcaaaaa agccatttcc attatattgg ctcattgaca actccgccta ccagcgggcc
    49561 aatccaatgg tttgtaatgg atcatgtagg aaaactgaac caagagtttc ttctcccctt
    49621 cgatggtcag tatgctcgac cgaacaaccg accaatccag cccttgaatg gaagagaaat
    49681 ttattatttc gaggagtttg agcaatgaat attcagcaga tgaaatacgt ggtcgctgta
    49741 gcaaacaatg gtagtttcag agaagcggca aagaaaatgt ttgtctccca gccaagtctt
    49801 tcagctggta tcaaagaatt agaaaccgag ttagggatca cgctgtttac tcgaacgaat
    49861 cgaggggcat atttaacaga agaagggcaa gacttcttaa atcgtgccga gaaaatattg
    49921 gtccagcttg atacattaga aactcactat ctaacgagtg aaaagagaga caatttttcg
    49981 atcgctgccc agcattatga tttcttaggt cctttgaccg cacagctact cgaggaattc
    50041 aaagacgaga tcaaacaatt tcggatcatg gagacgacaa cagcgagagt gatcgaagaa
    50101 gtcaaagagc agcacagtga gatcggcatt atttatatga atgagcataa tcaagctggt
    50161 atcagaagat atttacaaca aggagagcta agtggccgca gcattggaaa attcaaaacg
    50221 catatttttt taaggcaagg acatccatta gctgaacacg cagagatcac caaagaagaa
    50281 ttgttatctt atcctcaagt tcgttttaca caggatggaa ataatttccc gtatttttat
    50341 gaagacctta tcgagatccc tgatcaagaa acagtgattt atacgagtga tcgaggtact
    50401 ttgatgaata tcgttcttga aactgatgct tacgcttcag gatctggtat tgtgattgga
    50461 gggattcgcg agcacttgcg gttaatccct ttggcagaag gcgaactaaa tgaattttat
    50521 atcatttatt cggcgaaacg tccgctcagt gaaatcgctc aaagatttat caaagagctt
    50581 actcggttac tggaccagca gagctaagaa aagcagatga gaacgctgtt tattgaacac
    50641 cgttgatcaa aaaagagggg ttgtgacaga attttgtcac aactcctctt tttgtagcaa
    50701 acaatttata actaattgct gttgtgggaa agtacgcaaa tatgattcta gaaacactca
    50761 ttgattacct gttttgtttc atagctgtaa ttttttcttc ttctggtgtc acgataatcg
    50821 tcttttggtt ttcataaata acataaccag gcttggcgcc atttggtttt cggatatgct
    50881 ttacctgtac aagatcaacc ggcacttggg cagagtatcg gtattttgaa aaataggcag
    50941 ctaactcggc agcttctgta attgtttcat ctgagggctt gtcactttta atgatgacgt
    51001 gcgaaccggg tatatttttt gcatgaagcc aatagtcggt ttttttagct gtcttcattg
    51061 tcagctggtc attttgaaga ttgtttttac caacaagaat gagcgttccg tcagaagaaa
    51121 agtattgatc aggctgagat ggtttcttac gtttttgttt ttttaagttc ttctttttca
    51181 ggtatccttc tgaagtcaat tcttctttaa ttgcttcgat atccattggg ccggcaattt
    51241 ccagttgaga taagacagat tcaagatatt ggatttcatc tttggcttct tggatttgtt
    51301 cgccaatcag cttaacagcg ttcttcagct tttggtatcg atggaaatat ttctgtgcat
    51361 tttgatttgg tgtcaatgcc ggatctagtg ctattttgat tggccgatct tcttcataat
    51421 aattaggtaa aacaacttct tttgcgccgc gtggtacttg tgtcagaaaa gtagtaagta
    51481 gttcgccatt tctacggtaa ttctcagcgt tttcggattc tttgagtgtt tgttcacgtt
    51541 ttttcaattt atttttattt cgttttagtt cattttcgat ttttcgaatc agttctccgc
    51601 cttgttgttt tgcccgatct ttctctgctt tttcgtgata atagctatct aataattgac
    51661 tgagtgttgg ataagctgtt acagcagatg cctgttcgga caatacctga taagcaatcg
    51721 gtgtgaaaaa atctttattt tccgtttcgt aaaaagtagg aataggctga tggctgatgg
    51781 ctgagaaaaa ttcgtcccat acaaccatct tttcattagg acgttccagt aatcttttgg
    51841 tcagctcttg agcgatatcg aaaccgattc cctgaaactg acgttggatt gcttttggat
    51901 ctagttcagt ttgggaaagc cgctgaaaga ttttttcttt ctcggagcta aatgggttca
    51961 agacctcttg ttttggcgga gcaacatatt ctacaccagg aagcaaagaa cgataagtgt
    52021 tttgagagct tccaatgtgt ttgatcgcat ccagtatttt tccagtttct cgattaacta
    52081 agacgatcgt actatgacgt cccattaatt caacgatcaa tatgatattt tgtagatcac
    52141 ccaactcgtt tcgtttagca aagtggaaat ggatcactcg atcattttcg atttgttcga
    52201 tgtcttctaa aatagcgcca tccaaatatt ttcgaagcat catcacgaag ttaggcggat
    52261 tatcaggatt ctgataatcg atttgggtaa tctgtacacg agcataactt gggtgcgcag
    52321 ataataataa tcgatggttt ttgccgcgtg aacgaatcac cagcacgatt tcattttcat
    52381 aaggctgatg gattttcgag atccggccag atagtaacgt ttcacgcaat tcgttgacca
    52441 tcgcgtgggt aaatacacca tcaaaggaca tggttcattc ctcatttctt ttcataagta
    52501 tattcattat aacgaaaaaa agaagaaaaa ccaatttggc cgaatagtaa agcaaaagtc
    52561 tagaaaaatc ttggactaat tacataaaaa acagtgtttc tatcgttgaa atccaaattt
    52621 ttaggtataa taaaaaatag gagaaaaagt agaaaagaag gtgtttgtta tttgggaagt
    52681 agttaaatcg agagtacaag aaatgaatga acatttctca gtgtcattcg cagaaaatcc
    52741 aagtaagtat tttcgacgtc ttgtttttct gctcatctta tcattgctgt taacagttgc
    52801 aatgttttta ttgccggaaa aaatccgaga agagcaagta aatagtctcg tttattcgtc
    52861 cataccaacc agcaaattga aaccttttga ttacagcaaa gctgatcaga aaatcaaaca
    52921 aagccgggcg atatcggtga tgttcagcaa accgaacggg aaaacctatc aggacatgct
    52981 tgatgttttt aatgatcctg agcttatgga agaattgaat cgaccgatct tttattatcc
    53041 gatcgtttat gatgtacatg aactggaaca aaaatataac atacgaacgg atgaagtgac
    53101 atttattttc tttgatggag gaaaagaagc aaatcggata actgcgggta agggaggaat
    53161 aactgatttt gataaggagt tgatccctga actgaatcga cttccgttag ccaatatcaa
    53221 acaattagaa gaagaattgg atagaacggg agtaacttcc caaaattccg agattgctaa
    53281 ttgagacatt ggcgcgatga aacggtgggt ctatatagcg ctatttttaa ttatcagaac
    53341 aagtgatgtt tgatcttcaa aataacaaat aaaaaattaa aaaatatagt tgacgaaatt
    53401 caaaaattcg attagactta aatcaattct taaggaaagg gtgacctgta atgaacttat
    53461 caaaacgtat ttcagcagaa aacaaaaaaa attggcgcag ctggcgtaga taagttgtat
    53521 taatggtgag actttcccat agatacaact ttttagagtg catttctaaa gcttgtatct
    53581 atgctagaca tgaacattga ctgccgcata gattagaatc tatgcggttt tttgtgtaaa
    53641 gacaatgacc gctttggcca ttgtcttttt ttgtagactg gatacttgcc tgattataat
    53701 aaataaaaag attgactttg caggaggaaa aaaatgaaaa acaaacgatt aataacagtt
    53761 gtagtaatta ttttagttta tttagcaggt gcatttattt atggaaaagc ctcaggcgaa
    53821 aaagaaagta cttcgacgaa ccagacaaaa aaaacaacag ttgttggggt attgcaatac
    53881 gtgagccatc cggcattaga tgagatttat cgaggaatca aagatggtct gaaagaagaa
    53941 ggattaaaag aaggagaaaa tcttgagatc aaatttcaaa atggacaagc cgatcagagc
    54001 aagctagcca caatgagtca acagctagta caatctgatc cagatgcctt gattggaatc
    54061 gcaacacctg ccgctcaatc attagcaaac acgacttcat cgatcccagt tgtgttaggt
    54121 gctgtaacgg atcctgtggg ggctggatta gtcgcttctt tagagaaacc tggtggtaat
    54181 gtaacaggtg tttctgatca gccgcctgtt acttcacaaa tcaagcttgc ttctgatttg
    54241 ttgccaaatg caaaaaaagt cggaatcttg tatgcttctt cagaggataa ttcaaaatac
    54301 caagtagcac aagcagaaga agctgcaaaa aaagaagggt tagaatcagt aaaatatgcg
    54361 gtgccttcca ctaatgagat tgcacaaacg gttcaagtga tgagccgaca agtcgatttt
    54421 atctatgttc cattagataa taccattgcc aatgcgatgc aaacagtggt aaaagaagca
    54481 aacaaagcaa atatccccgt cattccatct gtggatacaa tggtggaaca aggtggacta
    54541 gcaacaatcg ggatcaatca atatcaatta gggcttcaaa gcggtaagat ggcggcagaa
    54601 cttgcgagtg gaaaagaaaa accggaaacg acgcctgttt atacatttga tcaaggggat
    54661 acagtgatca atcaatctca agctgatcgc ttaggaatca ttgttcccca atcgatgaag
    54721 gaaaaagcca aaatcattac agatgactcg cagcaagaaa catctaagga ggatgacaaa
    54781 tgatcgtatc agctatttca caaggcatgc tttgggcaat attaggatta gggattttca
    54841 tgacttatcg gatcttagac tttcctgata tgacgacaga agggtccttc ccattaggtg
    54901 ctgctgtctg tgtgacagct attactcatg gggtagcgcc gattgtagct acattattag
    54961 gcgtaggtgc tggcatgata gcaggactcg ttacggggtt actttataca aaaggaaaga
    55021 taccggttat tttagcaggg attttagtga tgtcaggatt gaactcagtt attttgtttg
    55081 tgatgcgaac accaaatctt tctttattga atcatccgat tctacaagac agcttccatc
    55141 gctttgggtt acctgattac tttgacacag tccttcttgg cttcatctct ttgggcatct
    55201 tgattgctgt actgctattc ttcttcaata cagatttggg tcaaggctat attgcgacag
    55261 gagacaacga aacgatggct cggtcgttag ggataaaaac cgatcgtatg aaaattttag
    55321 gtttaacttt gtcgaacggt gtcattgcat tatccggtgc gctgattgcc cagaacgacg
    55381 gatatgcaga tgtaaataaa gggattggcg taatcgtcat cggtcttgct tccatcatca
    55441 tcggtgaagt gatcttccaa gaattgacgc tagcagaaag attgattgct attgttgtcg
    55501 gcagtatcat ttatcagcta ttgattttag tagtaatcaa actagggttt gacacgactt
    55561 atctgaagtt gttctccgct atcatcttag ctatttgtct gatgatcccg caattgaaga
    55621 aagcactgaa cttaaaaacg ggttttgaga aggaggcata gtaatgactg atatattgac
    55681 aatcaaaaat ggcagaaaag tcgtaaacaa tggaatgaat gaagagaaag tactattgga
    55741 tgaaattaac cttacaatca gagaagggga cttcatcact gtccttggcg ggaatggtgc
    55801 tggaaaaagc acactgttca atacgatcgc tggtacgctt caactcagtc aaggatcgat
    55861 ctcttttaaa gatcagctga tcacaaaaga ttcagaagaa aaaagagcac gattcctttc
    55921 tcgagttttc caagacccca agatgggaac ggctcctcgg atgacagtag cagaaaattt
    55981 gctacttgca caaaaaagag gtcaaaaacg gaaattccgt ttacgtaact tgaaaaatca
    56041 aaaagaagat tttttcagac tatgtcaaga agtcggtaat ggcctagagc aacacttgga
    56101 tactccagcg ggtgaattat ctggtggtca acgccaagca ttaagcttgc tgatggcgac
    56161 gatcacagct cctgagttac tattattaga tgaacatact gcagctttag atcctaaaac
    56221 agctaaagca ttaatgcgat taacaaatca gcggatcaat gaacaaaaat tgacttgtct
    56281 catgatcacg catcgaatgg aagacgcctt gaaatacgga aatcgtctga tcgtactcca
    56341 aaaagggaaa atcgttcaag atatttctga aaaagaaaag caacatttga caatgggcga
    56401 ccttttgcag ttctttgaag aagtagtgga ttgatttcga cagaaaaatt cctttcagta
    56461 gaaggacttg aaaactcatt gacatcttcc tttgattcat cctatactta aaacaataaa
    56521 caattaaaca tatttgggtg ttttgtagga tcacgtagtc aatgatttta acaattaaat
    56581 agaatattct ttgctctgac aagtaaacga tgagaaaaga atttgctact ttgccaccta
    56641 gacgtttcgt actttttcgt atcaaacgct aagtggcttt ttttattgaa gtaagaacaa
    56701 aggaggaata agatgatttc tggacaaacc aaattagctg ggctctttgc tgatccggct
    56761 tctcacagtt tatccccatt gatgcacaat actgcttttg aagcaaacaa catcgatgcg
    56821 gtgtatttag ctttccaagt aaatccaatg aatttgaagc aagcagtcga aagtattcga
    56881 acttttgata tgttaggtgt aaatctttcg atgccgaata aaacagcggt tattccttat
    56941 ctggatgagg taagccagga agcgcaattg atcggagcag tgaatacgat cgtgcacgaa
    57001 aagggtcgtt tgattggcta taatactgat ggtgcgggct tcatgcaatc cgtgcaagaa
    57061 gctgggattt cgatcaaaaa gaaaaaaatg actctactgg gagctggagg tgcagcaaag
    57121 gcagtcactg ttcaagcagc gttagacggc gctgaggaaa tcgtagttta taaacgaaac
    57181 aaccaaacat tcgctcaagt aaaagaaaca ttcgaacaac tcagctatcg aacttcttgt
    57241 aagataaaag ttcgtgactt tgccgatacg catcaattaa gaaaagactt gaaagaaagc
    57301 tgtcttctag tcaatgcaac agatatcggg atgggaaaga aaatcaatca gtcaccgctc
    57361 ttagatataa gcatgatgca tgagaatcta gcggtttttg atttgattta tgcacctaga
    57421 gaaacacgtt tattaaaaga agcaaaaaaa gtaggagcaa aaggatacaa tggacttggc
    57481 atgttgatct accaaggagc aatcgcgttt gaattatgga caaaggaaaa gatgccgatc
    57541 catgtgattg aaaagctgtt tgattaaaaa aggataatag ataaagatct tttattctcg
    57601 cataaacggt ctcaagaaaa gtaatccttt atgtatcgaa gcatttattt cgttattttt
    57661 tattaaatta tcaaaaaatg ataggctttt gtcgattggt tatcgtatcc tttatttatc
    57721 agtaagaaac gaggaggaac cacaatgagt cggatggatc gctataagga catccataag
    57781 aaggcgaaac ctctgaagcg ggatacgggg agtatttttg ctagaagaga acgaaaacga
    57841 acgcgtatgg aaaatgagga agatgctacc cgtatttatg aaagagagca gcattcattt
    57901 gaacatttga accaatacga gcaacagata tacagagatg caccaagagc agaagaacaa
    57961 caaaaaaata aaaaattttt caatcggcaa aagaagaaca aaccgttaaa acctcgaaga
    58021 aagcgatcgt ggaaaaaaat cattctatcg attttgcttg ttttgatcct ttattcaggt
    58081 atctcatttt ttattggtaa atatgtagct gaacatgaca caagcttaga ggcagcgact
    58141 tcagaaacct ttcatgggca aaaagcgacg aatggtgcgc ataatatttt gatccttgga
    58201 agtgatacac gcggagaaga tgccgggcga gccgatacga tcatggtctt acagttggat
    58261 ggtccttccc ataagccgaa actgatttct tttatgcgtg acagcttcgt tacgatccca
    58321 ggggtaggac aaaataagat caattcagct tatgcatacg gaggagccga tctagtgcgt
    58381 caaactttag tagaaaattt tggaatcgat tgtcaatact atgcgaaagt cgatttcaaa
    58441 tcgtttgaaa aagtgatcga tgccttattc atgaacggag tgaagatcga tgcagaaaaa
    58501 gatctgaatc tggatggtgt agatattaaa aaagggggcc agaagatgga tggacatgtt
    58561 cttttgcagt acgcacgttt ccgaatggat gaacaaggag actttggccg tgtcagacgc
    58621 caacagcaag taatgaacgc tattttcagc caattgaaga atccattgaa tctgatccgt
    58681 gcaccttatg cagctggtaa agctttagga tatacatcca cagatgtcac tgcttggttc
    58741 ttgacgaaaa acctcttttc gattgcacga ggagctggtg gcattgatcg aataagtgtg
    58801 ccagtagacg gttcgtggaa ttatgggcaa agtgattatg ctggcagtat tcttgaaatt
    58861 gataatcaaa tgaatcaatc tgctattaca gattttttga ataaatgatc ataaaaaaga
    58921 tcaagaagaa gctgcgcaag gcttttgtca tgaatcaatc aaacgattca ctcgtaaaaa
    58981 acgctttctt atggtagatt taaccaagag aggagctgtt tacattggat atctatctga
    59041 acttcaaaaa tgagtcgcgt gaagccatcg ctttttatga aaaagttttt cagactgaag
    59101 catcaggtat caccacatac ggtgaaatgc ctggaataga acaaatgaac ttgccagaag
    59161 aagcaaaaaa tctaatcacg aatgcacggc tgaacattta tgatacatta gttatgtttg
    59221 cagatacgcc agaaggaatt ggtcctgaat tagttgtggg aaataatgtt tctttagtca
    59281 tccagacaag aaatgaagaa gaaacgaagc gcctatttga aagcttagct gagggtggta
    59341 agattgagat gccactggat gctgttccgt gggctcagtt gtatgggatg gtaactgatc
    59401 gatttggcat caactggcag attaaccaag agtgacagct aaagatgaat attttctgga
    59461 aacatcaagc agcaaacgga tcatcgtttg tttgcttgat gtttttgtgt ctatttcgac
    59521 tatacggtat ctttttttta ttgccagatg cgaaaatatg ttccataata gagaagtccg
    59581 caaaaaatta agtaacggag gtcagaagat gaagacggtg atactagctg aaaaaccttc
    59641 acaagcaaaa gcctatgccg aaaccttcca acagcaaaag cggcatgaag ggtattatga
    59701 aatcagcgat ccattgtttg ctggtgaagt aaccatcact tatggttttg gacatttggt
    59761 agatatggtt gctccaggag cttatgaaga gcgttgggca aaatggtctt tagaaaattt
    59821 gcctattttc ccagaaacct tccgttacga agttccaatg gacaaacgtg cacaattttc
    59881 gatcgtaaaa cgagaattgc agtcagccga taccatcatt attgcaacag atggtgatcg
    59941 agaaggtgaa gccatcgcgt ggtcgattat tcgacaagca aacgcatttt ctaaggaaaa
    60001 gaactattta cgtttatgga tcaattctct tgaaaaagaa gcaatctatg aaggttttaa
    60061 gaatctccaa cctggaaaga actattttcc aaaatataaa gaagcgcaag caaggcagaa
    60121 cgctgattgg ctgattggaa tgaatggaag tccattatat agcttgctac ttcaacgaaa
    60181 agggattgat ggcagttttt ctcttggtcg tgtacaaaca ccgacactct atatgattta
    60241 tcagctgcaa gaagaaatca aacacttcaa aaaagaaacg tactttgaag gagaaggttt
    60301 aattacgaca aacaaagggc agttttctgg aaaaatcgta ccaaaaaaag catttaaaac
    60361 ccaagaagag ttaacgaagg aaatagaaaa acttggagcg catttaggta agcaaaacgg
    60421 acggattctt gaagttacga aaaaggaaaa gaggttgcat agtccacgtt tattttcgct
    60481 atctagtttg cagacaaaga tgaatcagct aatgaaagca agtgccaaag ctacacttga
    60541 tgctgctcaa ggattgtacg aaagcaagtt tctaagctat ccaagaacgg attcctttta
    60601 cattacagaa aatgagcatc agtatctggt taaacatctg caaaattata aaaaattttt
    60661 aggaatagaa gaagtacaga caactgagat caaagcaaag aaacgatatg tcgatgcgaa
    60721 aaaagttcaa gaacatcatg caattattcc gacaaagacg attccgactc ctgcacgctt
    60781 tgcgaagctt tccaaattac agcaagcaat ctatttgcaa gttttgcgca ctactgtggc
    60841 tatgtttgct gcagattata tctacgaaga gacgaccgta atgacgggcg tacagcaatt
    60901 aaagctaaaa agcatcggaa agatcccatt acagcaaggt tggcaggaaa tcttacgcat
    60961 caagtcaaaa aagaaagaag ctcagactct tcctcctatc gtagaaaatg aagaagcaga
    61021 agtagaactg aaaagtatgg agaaagaaac acaacctcca aaaccctata ccgaaggaac
    61081 gttgatcaca gcaatgaaaa ctgcgggaaa aacgctggat aatgatgaag cgcaagaaat
    61141 tctcaaagaa gtagaaggaa tagggacaga agctacacga gccaatatca ttgaaaatct
    61201 taagcagaag cattacatcg aagtcaataa aaacgaaata actgttacgc caaaaggaat
    61261 cactctttgt aaggcagtag ccaatgaacc attactgacg agcgcagaaa tgactgcgcg
    61321 ctgggaaggg tacttgagaa aaattggtaa acaagagggg acaccatctg tttttttaga
    61381 gaatatcaaa aaattcatcc tacatttatt ggcagaagtt cctaatcaaa tcgacaaggt
    61441 agacttttca gatgagatta gcgggacacg tcagataaaa gcgatggaga agaaaaacga
    61501 tcaattagga agatgtccaa aatgtaaaaa gggaatagtc atgctttatc ctaaaatcgc
    61561 tacatgcttg aatccggaat gcgacttcaa attgtggcca acagtagcta aaaagaagct
    61621 gactaaaacg aatctcagag atttgctttc aaaaggaaag acaagtaaag ttgtcaaagg
    61681 ttttactggt aaaaaaggga aattcgatgc agttttagta ttaaaagaag atatgacaat
    61741 cggtttttct tttccatgga tcgaaaacag tggaacaaaa gcagttgatg gaaacaaaga
    61801 aacatcaaga aaaaataatg aaaacgaagt agagtaaatg aaaacaatga aatagcggaa
    61861 tatttattcc gaaaagtgag aatataaata ccaataaaac taaaaaatat cagtgaatca
    61921 agcaaaaacg ttggggtagg ctgattttat atagatagca aggagttttt ttatttgaaa
    61981 aaagtgcatt catttttaaa ttatcttatg ggagcaatca cgattgtggc tgtgtaccaa
    62041 gcatttctaa agtatcctga ttacgggacg aagttgttgt cattgacgca caatcaaaca
    62101 gcatattcac caacgttgat tttatcgact ttgactgcag gagctaaaag cgctacttat
    62161 ttttttcata aaacgacaag catgctaaag cgaaagaaac agcgaaaaca attgttggtg
    62221 gtggtagcgg ctatgatcat tatttatttt ctaccagtta ttacacaagt atttcatcaa
    62281 tattttggtt aagaatacga gagtttgtga caaaagtcat gtcacaaact tttttctttg
    62341 tcattttttc gtaagaaaaa gcaaaataaa agtatataca ttgattaaaa gtatatactt
    62401 ggatataatg tacttactat agaatgagaa tggtatagag ggtggcgaaa tatgaaaaaa
    62461 atgaaaaagt ataaaattag aaaatctggc aatagcgatg taacaactat cccaccagaa
    62521 gtaaaagaat tcatgggtgt gcagacaggt gacgcaatca gttatgtttt tcaatctgat
    62581 ggttctgtac gaatgatcaa agcacaagaa gaacctgata ttgattcatt agttgattcg
    62641 atcatgaatc aatatgaaga cgcgttaaaa gatttggttg atttatgagg tatctttcga
    62701 ctaaagaaat tatcaaaatc aacgcgaaag ttattttacg tcactcgcca ggcgaaatga
    62761 tcggtgtaaa agatgcaaat gcattagata tggccgtcaa acagccttca caagcagtat
    62821 tcaatcaaga actctatcct gaaatctatg aaaaagcggc gattttagca attaatttag
    62881 ctaaaaaaca tccttttcat aatggcaata aacgaactgc tcttgttgcc atgttgttgt
    62941 ttcttcaatt gaataattgt ccagttgctt tttctcgtca agaagctgtt gattttatca
    63001 taatgattac cactagttca ttggatttcg attcactaaa gtccaagata accagttatt
    63061 tacgacaaac cgcaataaaa tgaacgaatt aaaaacttta tttctcaatt agatcagcat
    63121 gtcattaagg aatatgctga tttttttatg tttaatcata caaaatagtt ttttcataaa
    63181 aatatggaaa atatggatta attttgaaat aattgattta aaaggaatac aaaagagtga
    63241 atagtaggaa atagtggttt agaactagtg ggctgatttt ttatatattt tagtttacat
    63301 aatatatatt atacaaagta gatttaaaaa atgataaacc tctctcgaac ggttttttat
    63361 caaaaatcgt catttttcca tcaaaatctg cttatgcaaa attataaaaa aattttgcag
    63421 tttttcttac tttttttgtc gtttaattga atgataaaaa ccagtttctt tattcagtta
    63481 atcaaatatg aacgaattag atatgaatca ttgtatttgc cgttgcggaa ggatttttta
    63541 attaaccaca gataaacatt gataacgatt cgcttattgt aatcaacaga ttaaatttca
    63601 tatgttcata tgtacggata cattttcgga ccgattaatt ttaaccattc tgtttatttg
    63661 cttacctatt agatatcaat aaaaaactgc ttaacagcta tttcagctgt taagcagttt
    63721 taggattttt agttcatatt cattgtttct tataatacag tgttcaatac ttctcttaaa
    63781 gcacttgttg atttgctgaa cattgctttt tcttcttcgt tgattgataa ttcaacaact
    63841 tcacgaacac cattttcgtc aacgattgat ggcacacctg taaagatatc tttttcgtcg
    63901 tattcaccag ttaaataagc agatacaggc aatacagctt gttcgttatt caagatagct
    63961 tttacgatac gtgcagtact cataccaata ccatagtaag ttgctttttt acgatcaatg
    64021 atctcataag cagcattacg gactttatca gcaatcacat ctaattcatc ttgggcaata
    64081 cgatggtctt tttctacaat ttcaaacact ggtttaccac cgacagttgt atgtgaccat
    64141 gcagcaactt ctgaatcacc atgttcacct aagatatagc cgtggacgct gcgtgggtca
    64201 acttttaatt tcaatgcgat ttctttacgg aaacgagttg tatccaatgt tgttccagta
    64261 ccgataacac gtgatgttgg cagaccagat tcgttccaag cgatatatgt caaaatatct
    64321 actgggtttg aagcaacaac gataataccg tcgaatcctg atcccatgat ttcttttacg
    64381 atttggcgca tgattgacgc atttgtttta actaaatcta aacgagattg accaggtttt
    64441 tggttgatac cagctgtgat aacaacgata tcagcatctt tacattcttc atagccacct
    64501 gaccatacag ccacattttc atcgccccaa gccataccat ctaaaaggtc caatgcttcc
    64561 ccttctgctt tttcttgatt cacatcgatc aatactaatt cattagagat tccttggttg
    64621 atcattgcat aagcgatact tgttccaaca aaacctgtac cgacgattac tactttacga
    64681 cttgtttttt tcatgaataa acagctcctt ttcataacta tttgttactt tcatgaaaag
    64741 tatagtcttg tttattcatt ttatcaacat gttaattata atagttgtta ttataatttt
    64801 tgatggaaac gaattcctga atacgcataa cagataaatg acaaatgttt acacctatat
    64861 cccctgataa tacaaccaaa agtttagaac tgaaaataaa tccaaaaaaa ttcgctgcaa
    64921 cgatataata agacagcaat cgcacctaaa acaattttcc ttcatatgct ttagtgaaag
    64981 attttcttct taatgtttat ttctccattc attgggagta catgttgaat acttcttgaa
    65041 taatttgaat aaagaagtcg tttgtttata tccgatacga ttagcgattt cttccaacga
    65101 aaaactcgtt tcttgaatca gttccaacgc aaagtcgaat tttttcttta gaaccagttc
    65161 ttgaaaggaa gcatcaaaat acattttcaa tttattacta agatagaccg gattgtagcc
    65221 aaaatgagct gctggttctt ttaacgtaca agaagtaaaa tgcttatcga tataatctaa
    65281 tactctaagc atctcatttt ctgcgcatcc atctgctgat gaaaagcttt gttgttctaa
    65341 taaatgagtc aattcgatca gtaatagtga tgtatagaga tttaacgaac ggatcttatg
    65401 actgtatgta tcaaaataaa tcaacagcat attttcaatc aaatgcgtca aacgacttgt
    65461 ttttccgccg cgaaaatgaa tgaattgtgt atgttcccct ttttcttttg atgcatttat
    65521 caaaaaagaa gaaagtagat tatttgtgct tgccaaatga ttcaatatct ctgtagataa
    65581 ggtttcttct tttaataaga tgttgattaa aatatcctct tgccctgtgt attcgattct
    65641 ttgagtagct tctttatcaa tgatcaccac gtcactttct tccatctcta tcctttctcc
    65701 attaataatt tgtaaacatg taccgctata catataattg atttcaacaa aatcatgtgt
    65761 atgctcggga acttccgcat atcgacggtg tttactgata tgaatatttc cctgttgaaa
    65821 tagatcccct cggtggattt ttggcggtgt ctgaagatgg taagctaagt ttaaatcatt
    65881 gatattcatt ccttgttcac gctgcagagt ttcgattgat tcttcgttac gcaaaaaaaa
    65941 tgtctaattc agctagttcc aattgactcc ctcctttttg taagcggttt ctttcgctta
    66001 ttagttcacg ctgtactgct attatatgca aagctatttt tggtttcata aagactttct
    66061 atggttggga catgaacttc ctgtgattcc gctattgaga gaataaccac ttcatactat
    66121 atttttgata agcgcttaca aaaatgaaag gaacatgctt atgaataaaa agatgatgaa
    66181 attttctctg ctgtcagtct ctcttatttt gacttctgct ggtgcagtca cgtctgcaat
    66241 tcccagtatg tcagctactt atgccacgcg ttctttatct gctgttgaat tattaacgac
    66301 tgctccctct tttatggtca ctatttttgt tttgattagt agttttattg ctaggaaaat
    66361 cggacaaaaa caaactgtga tcatcggatt agtgattgca tcgatttgtg gtatcctccc
    66421 cctcttctca caaagttaca gtattgtctt agcttcaaga gctggactag gtattggctt
    66481 tggattgatc aattctttgg cagtcagcat gattgctgcc ttttatcacg gagatgaacg
    66541 agcctctatg attggttttc aaagtgcatt tcaaggattt ggtgcagctt taatgacatt
    66601 tgttgcagga cagctagtac agttcggttg gcaatatacc tttttagtct acgccatcag
    66661 cttacctatt cttgtcttgt ttgttttgtt tgttcctaca cctgaaacaa ctgaacttca
    66721 atcgcatggc acagccccaa caagacaagt aatcaaaacg aatcctaaaa tgattagtta
    66781 tggtattttt ctcttgctgg tcatcgtggt atataatgct gtatcgatta aattagccac
    66841 agtcctgacg acttattctc taggaaacga aactaatgca gcgaatatct taacaatcat
    66901 gcaattggca agtatggctg ccggagtttg ttttggctgg cttcagacaa aagcacatcg
    66961 ctatatgctt acactttctt tactactgat gggaatcggc tttgtattga ttgccactgt
    67021 gataaacctg tatgtgattg ggtttggtgc gatcctaaca ggaatttctt tttcgttatt
    67081 cattccttac ttattcaacc aagtaagtat ccaagcgccc aagtacgcgg agagtttcaa
    67141 tacttcaatc ttattggttg gcgctaattt aggtactttc attgcaccat atggtttgct
    67201 ctttctctct tttgcttctc ttgggagtaa aacacttgct gttttcgtca atggcagtat
    67261 cttgctgttg attagcgcgc ttattagcct tctagtagta cacaggacga atagaaagac
    67321 agtgataaga gtagatgaag aaataaaaga acaattgaca acaaaataaa tttgatggag
    67381 gtcgtgtaaa atgacaaatg aaaaacataa agatagatgg ctttggtatc ctggagactt
    67441 tgagatccgc catggactgc tacaaaattt ccaacgggaa gaacgaggtt ttgactggcc
    67501 agcgtactgg tacatggatg attgccatcg gaacgtcaag tttaagagat attactttct
    67561 tgatcaacct tccatgttta aagtgacgat ccaaggagtt gggtatgtag aaattaatgg
    67621 acaaaaacat ccatgtggaa aatggctgac ttgtccagct ggtaaagcaa aaattcgtat
    67681 ctttgtagga catacatcag ggctaccagc gatgtttatc gagggagacg aagtaaaatc
    67741 agatattggc tggacagcaa gtaattttat tgaagaatat ccagctggct ggagtccgct
    67801 ttatgtcgat atcgcaaaag atccaaatca aatctattat caaaaagaat gcgtccatcc
    67861 agtcactgaa acggagattg gcggaggaat cttattcgat tttgggcgag cagtgaatgg
    67921 ctgtgtagaa ctaatatctc tggatgacca tatcgaatct atcactctat gttatggaga
    67981 gtccgatgta gaagctatgg atatcgatta ttgctactat aagcaaatag gaatgacaag
    68041 aaaaatgaaa gctcgtaaac gcgcttttcg ctatttgttt ataccagagg tttctactgg
    68101 aatggttcaa cttcaagttt ttcatgaatt tttatctctt tcgaaacaag ctgctttttc
    68161 aagcgattct tctttactga atcagatctg gtcagtatca gaagaagcct tcaagttatg
    68221 tagtgatttg ttttttatcg acggtatcaa acgagatcgc tggatttggt ctggagatgc
    68281 gtatcaatca ttcatgatca atcaatatct tttttttgac gaggaaatca ataaacgaac
    68341 gatattagca ttaagaggac aaaatgaaat caagcagcat atgaatacta tcgttgacta
    68401 ttcgatcttg tggttgattg gtatcgaaaa ccactatatg atgacgaaag actatgaatt
    68461 tttacgcatc gtctacccta agatgcaatc attaatgaga tacttgatgg agcaaacgaa
    68521 tgaattaggg tttatttatg ggagagcaaa agattggata tttattgatt ggtctgaaat
    68581 ggataaagaa ggaacgcttg ctgcagaaca agtcttatta ctgaaagcct atcattccat
    68641 gatcacttgc ggagatatct tagctgaaac atatggagag aacgaaaatt atcatactca
    68701 tctctatcaa gaaaaatatc aactattgaa acaaaaccta tttgactact tttgggatga
    68761 agaacttggc gcttttatcg attgctatga atctgggaga agaaatgtga cgcgtcatgc
    68821 gaatattttc gctgttttat tcgattttgc taacaaagac caacagcaat cgatcttaaa
    68881 gaatgtctta ttgaatgaca ctatcacaca gattactact ccatatttca aattctatga
    68941 acaagatgct ttatgcaaac tcggacagac gaatatcgtc taccaaacga ttcttgatta
    69001 ttggggaggg atgttatctc atgatgcggt aactttctgg gaagaatacg accctactca
    69061 aacaggaaat gaaaagtaca gtatgtatgg agatccttat ggaaaaagct tatgccatgc
    69121 atggggagca agcccaatct atcttttagg acgctatttt atcggtttgt atccaacaaa
    69181 gccaggttat gaagaattta tgatacaacc taatctagat tcatttgaaa cactgaattg
    69241 tacgctacct atcaaagatg gaaagatttc gcttgaaaaa acaaaagata agatcgtgat
    69301 tacttctgat agaacaggcg gaacagtgaa aatgaatggt aaaagttatc ctcttctagc
    69361 aaaacagcca cttgtcttaa caagctattc aaaaataaat gcataaaaca aaaaactgct
    69421 tgcgacatat cagaagactt taaatcttct tctgatattg tccaagcaat ttttattttg
    69481 aagtttgctt aattctgttc tttagaaaca atgtttgttc tttttccggc aaaaagaaag
    69541 ctagataata cgacaagtaa aaagaaagcg atgattccaa ctgtcaaggc tatttgaccg
    69601 tattctccta gtttcccaag tacaagtcct ggtactagat aatcagtatc ggcaaatgta
    69661 gaagaagcga ttttaagatc tcctaatact ggaaggataa ataatggcag ccaactaatc
    69721 aataagctat tgaaagcaga acctaatacc gcccctcttc taccgccagt cgagttgccg
    69781 aagacaccag aagcagctcc gcagaagaaa tgtcctacta ccccaggaat gataactgta
    69841 gtttgcaagc ctatcatagc aaacatggat agtgttccta caaagaagct aacaaaaaag
    69901 ccaattaata cagcattagg tgcatatgga aaaacgatag gaacatctaa tgctggttta
    69961 gaatcgggta cgagtttttt agcaatccct tggaatgctg gaacgatttc tgccaacacc
    70021 attcgaaccc cttgaaggac gataacaaac ccagctgtga atgttccagc ttggatcagt
    70081 gcaaagatta cagcgttgtc tccattgctt agttctcttt caacaaatcc aggaccagct
    70141 aacaaagcaa gtgcgacata tacgatcgac atcaagagca tgatcgatac agtagaatct
    70201 cgcaaaaagc tgaaactttt aggaatcttt atgtcctctg tagaacggtc tttgtttcca
    70261 aaaaactttc cgatccatcc actagaagca taaccgatat ttccggtatg ccctaacgca
    70321 acttgattgt ttccagtgat gttcttcata aacggttgac aaagagcagg cgtaatagtt
    70381 aatagtgttc cttcgaagac cccgcctaat aagatcatcc acaaatttgc agtaaacccc
    70441 gcgcttacta aaatgactgc tgttagacaa gaaacataaa gcattgcttg acctgtaaga
    70501 aaaatgaatt tatatcttgt gaatctagca agtaaaatat tcacgatcat ccctactagc
    70561 atgatcaaag cagtaggtgt accgtattgt tgcaaagcaa tcgccactac tgcttcattg
    70621 ctaggaacta ctccttgagt atgcagagct gtttggaaca tcgaagcaaa aggggttagt
    70681 gaattactga ttaaaccagc accgccgatc agtactagga atccgacaaa tgttttgata
    70741 gttccttgga tcacttctga aaatttcttc ttctgaagaa cgagaccaag cagtgcaatc
    70801 aaagaaacga ggatggaggg ctgactgacg attgacacaa acaaattcaa caatttatcc
    70861 attgatagtt cctcccttga atcattttta ttcttttgac aagatcttcg ttactttttc
    70921 ttttacttct tcgctgtcaa tgatcgattg caaaggtacg agcttttcat caggtataga
    70981 agttagcgta tctttcaaag aatgctcaat gaagaaataa tcagccatat cgctagatac
    71041 acttccaaca tccgtatgac ttacttcaaa ctcgtccaca tctgctccaa gatctgcaag
    71101 tgcttgtttg atgttcatct ctaccataaa acttgttcct aatccacttt gacatactgc
    71161 aaaaattttc atatcattcg tctccttttc ttatgatcgc taatatttct tcttttgttg
    71221 atgcgtgtaa taaatcttga agattttgtt tattagaaag tagctttgtt aaatttgcca
    71281 aagcccgaag atgtgcctca ttatcgatag cagctaaaca gatgaacaaa ctaatgggat
    71341 gggctggatt atcaagcaga tccacaggtt gttgaagttt cagcaaagac atccctactt
    71401 cgtttacgcc atcttctgga cgagcatgag gtaatgcgat gtgatcgcca atatggataa
    71461 acgcaccata ctgctccacg cgctcgatca tagctttgat ataactttct tctattttat
    71521 tttctgataa tagcggctgt gcggcaaatg tgattgcttc ttgccagttt tttggttgat
    71581 ccgataattg gatcatttct tctgttaata attcagatag cataggacgt tgatcctcct
    71641 tcttttctaa aagtttgttc attttacgat ttaatacttg atagagcttt tcttcgtcta
    71701 cgccttcttt taattcgata taaggcagta gcgccgacaa caattcttgg gcagaaggca
    71761 ttgaaaatcc tggtatcagc caatctcttt gtacttgatt gatcagctga tttttttcca
    71821 agtcagtcat gattggatgc agaacataga gagacttttt cttatcagta agtgtgagag
    71881 gtacagtaga aaaaataata tcgtaagaag tttcgtcaac ctcgtgtaag cggtcagctg
    71941 aattagtctc tgtaaaaaag atcatcggga ataattgctg aagttcactt tttaagatca
    72001 atgaagagct aatgccactt ggacagacga tcacggctct tatttttcta tttctttctt
    72061 ctgcgtccgc ttttctgatc tctccgccaa atagtatagt gaaaaaacca atctcttctt
    72121 cagggatgct cttccctatt cgtctttcta acggaagtaa tgcttttctt gtcatttcgt
    72181 agatggaagc atattgatgc ttaatttgat cgatcatcac attaggtaaa taaaaagaat
    72241 aggaaatacg aaaataggca gggaccaagt gataaaagac attcatcaat aattcacgaa
    72301 acgaagcaaa ttctacggcc atcaggaatt ccatgcgatg gatgacttct gaagaacatt
    72361 ccagcaaaaa atcaagtgca ggctcctgga catctccttc aacgatcgat gctaagcaaa
    72421 gagagaaaaa tataatctcc gaattttgat gttcggaata gccaaggcca gtcaatactt
    72481 gtcgactaat gtcagtgact gcaagagaag ccaacagttc agcatctttt accagttcca
    72541 tggatgtttt tccaattcga atggttgtca aactcaaata ataagctaat tcatccattc
    72601 gatttggtgc gaatcgaaga tctgatagtg aaatcgtttt atctagcaaa tctctaactt
    72661 tggcgtaata atcaatggaa agggttgcgg ttaaatacgt gagcccaaat cttccagcaa
    72721 gtgactgcat caaacgagca gtgcgatttt ttgcttctct tcttaatatt gcttctgacc
    72781 ctagcagcct aaaaccagtt ttcctagtat acgacagctg acaatcttcc acttctaatt
    72841 catttcttat ctttttgata tctgacagga ctgtcccttt agaaacctga agaaaatctt
    72901 gaaaatgaaa aacagaaaca gacgttgcag tcgaataaac catcaaataa agaagatctc
    72961 ttcgctcttg ttcggtatac acagcttcaa atcgagtaat atccaaaaat ttttctgccc
    73021 agtcgattgc tcggaatttc tttatacgga tacgggtatc atccacatat tcggcaaaag
    73081 gagttaattg tttgatcgcc tcgcttgtat ttacttcgat gatctctgct gctttttcta
    73141 gactgatttc tcttggtgtt ttcaggagat aactaatttt gtagttcaca gcgctgttca
    73201 cctcctactt atattctagt atggtagcgc ttttaaatcg aggggtgttt ggtacaactt
    73261 acagttggat tttttttacg actgtaccga acgatttcta tcgaatggat acagaaaaaa
    73321 agcaggtgac caaatgtctc ctgcttttat gaattgattt cgtccgatca atgatgatca
    73381 tgtttatttt ttgaggtcag ttcttcaaat ttttctttga catcttcaac cgctttttcg
    73441 atatcttttt caaagtgatt gggctcatta ggctttgttt cctcttctac agaatttgaa
    73501 ccagataaag ctgtttcttt attttctgtt tcaccagagt ttgtgccagc taatgattct
    73561 tctttgtctt ctgtttcacc agaattagtt cctgctaaag ccacttcttc ttctgtttta
    73621 ggggccattg aacctcttaa tcgtttgtgt ttttcaccgt cataatttgt catgttggtt
    73681 cctcctagta gttctatact ttttcatcgt acaataagac agtttatgcg tcaaaaaaca
    73741 aacccgattc ttacaaatat gtcatatttt tccttctgat tcttgaatgc ttttgattcc
    73801 ttcttccaaa attttcattt gtaagattgt ttcttcatct ttgatgactt tttctgcgcc
    73861 attaactaat gtctcgtaaa tcccttcgta gacacggctg taatcaccaa cctcggaaat
    73921 tactttttct tcatgataac tcccttgttc atctatatag gtcaatacac cataatgttc
    73981 gggcaaatcg ataccaaagt cttcatgatc aggcatgtaa aacattttca gatgttcttc
    74041 ttgtcgatct tttgtttgtt tcacgaacat cccttttttt ccgtaaacga cgaaacttgg
    74101 acgttctttg atgcggaaat aactagattt tatcgaaatt ttcatatttc catagaaaaa
    74161 atccagatcg aagtaatcat tcattcgtcc tttccctaaa agttgacgta catcatagct
    74221 gatatcatct ggttcgccga aataagaaag aacttgatct actgtatgac aagcatgtcc
    74281 atataaatag ctgttcgcag tagaaaattc atgcgtattt tctggtactt ccggtctgaa
    74341 ataatcataa tgcatttcta cttctaaaat atcccctaat tttccgcttt cgattacttt
    74401 ttggactgtc aagaagtcag aatcgaaccg tctgttttgg taacattgga taaatagacc
    74461 tttttctttt gctaatgcaa ataactcttc tgcttccttt gacgtttctg caaaaggttt
    74521 ctctactagt acattcttac catgttcgag tgccattttt ccatattcat aatgaaattg
    74581 actaggtgta gtgatgacaa ctaattggat ctcaggatca tcccaaagat cagacagctg
    74641 atcagtatat tcgatgtctt cgaatgtttt ccacactggt tcagatgggg aaaaaatttt
    74701 tttgatattg attttgttcg taagttttct tgaaaaaggc aaatgatagc gatttgtact
    74761 ctttccattg ccaagataag cgattgtaag catgagtggt gcctcctttt attgatgttt
    74821 tcagtatatc ctagaaaagc gcagaaagta tttataatag ctattctggt aaaaaagttc
    74881 agaaaaacac ttgttatcat tcggtttggt gaaattaatg ttcaaagtaa ggataaaaat
    74941 caaaaagata gtagcggaac caagtgttta tcagtaagat agaatttgga ggtcagatca
    75001 tgcttattga agaaaaacta acaaaacaag agttgtttac cacgacagag aaacggattg
    75061 tcgattacat ccgtaggaat atcgaagctg cagtctacat gacgattgaa gaattagcaa
    75121 aagctacgta tacttcccat tcagctatca ttcgtttatg caaaaaaatg ggattcagcg
    75181 gatttaaaga attccgtttc gagctggggc gggaagttca tcaattgatt gctcagttca
    75241 atcaaacgat tgatcctaat tttccttttt caagtgaaga tagcactcag caaattgcaa
    75301 agaaaatggc ggatttatcg atccagtcaa taaaaaaagc acaattgcag attgaacatg
    75361 aagatttaga tcaaatcggt aaagtattga acaaggcaca gcgaatcttt ctttttgcaa
    75421 aaggtgattc tcaaatcacg gcaaggaaat tccagaataa aatggtcaag ttgaataagt
    75481 ttttgatcat ggctgaagaa tacagtgatt cttcttggaa tgcagccaat ttgactagcg
    75541 aggattgcgc catttttatt agctacagcg gtcgcatcca tcattatgaa cgaataatga
    75601 cctatttgaa gcatgtagag gcccctacct tgctgattac cggaaatcag cattcagaaa
    75661 tggccaaaca ggctgatatg tgtcttgtta tatcgcagga ggaatatgat tttgctaaag
    75721 tagcgacttt ctcctctcaa attgccttcg attacgtatt aaatacgtta ttctcagtga
    75781 tatattctca aaattttgaa gagaatatcc gcaacttgaa aaacaaacaa gaattgatgc
    75841 aaaaaggcct tcttaaagaa tatcggaaaa cttaagaaaa gatcctcatt tagtaataag
    75901 aaagatagtg tttgataggc tctctttgat atcatcataa aaacaggctg aagttttctt
    75961 tttcaaacct cagcctgttt gtttacggaa cgattcgaat agatatatta tagaaatgag
    76021 gaatagctaa cttctgtttc ttattttgaa cgattgacga aaaagatata taagaaggag
    76081 gaaaatcagt atacatgcag ctaacaatcc atacaggaaa aatcgtttgt tctcggatac
    76141 tttctgtact tctttttgaa cttcttttgc atgataagga acccggtgcc ctctgacaag
    76201 taagcgatga ctgttgacca tataaggtgt acaagttagg agtgtcacga aatcctgccc
    76261 tttttcgatt ctcagatctt ttgtatcagt cggttctacc gtcttgatct gatcgacttg
    76321 ataagcgagt gtccgtccat tgatttcgat atagaaagaa tcacctaaag tcagtttcgg
    76381 taagtctgtg aacaactttg cttgaggcag tcctcgatgg ctggaaataa cggcatgtgt
    76441 attttctccc cccgtaggat aagacgttcc ttccaataat gaggcacctt tttctagcag
    76501 cacagtattc gtttcatcaa aaattggcaa atggacattg atctcaggga tcgtcaaaat
    76561 accgatcgta tgtttctcaa aataggttct atcctttttc gcaggttctt ttttcttagt
    76621 aaagggatct gcacctggat ttccaccttc ttttgctaat tgctggtttt tctttgtcat
    76681 gttttcttgg attttagcca tgacttcttc attttctttg accgcttgtt gctgataatg
    76741 agagatgatc tgctgatcca gataattatt cagcgcgtcg ctaacaaatg gataagctag
    76801 tatccctatt cctgagagaa gcaagaaaag catcagcaga tctagaagtt tccttttctt
    76861 tggtttcatt tgtgttctcc ttgtgatgac tgtttgataa taaaagaaaa aggagcattt
    76921 gcgtaatccg ctgctccttt ttccaattct tgcaaaaaga ctaaatctga ctgtgcttgc
    76981 gtctagcaaa gtacagtcca gcaatcagta gaaggactac tcctgcaccg atatacacat
    77041 agattccctt accgcctgtt gaaggaagtg tacctttgtg tttatttggt attttttcag
    77101 gagaaatcaa ctgtcctgct gttacataag attgttcatc gatagtaaat gctacacggt
    77161 ttgttaatgg aacatatttt tctggcgctt tcgtttcttc cagatagtac gtgccatatt
    77221 ttagacctgt cacatcgatt aaaccgttac ttgtggttgt aaaaaccgtt gctgattctt
    77281 tcgccgatac ccagctgacg gcttttgtgg atgggtcgat cgataaatat ttcgctgtgt
    77341 cactatcttg atcacgaacg acgaattctg ctccagcaag tgtttggtct gatgtaacgt
    77401 caccatctac tttaacaaat cgtttgcccc cagtaacgac atcgactgac ggtggtgttt
    77461 gatcatttgt atgcccgtta tcgacatttg cttggttaga aaatcctttg gttggatctg
    77521 ctttttcgtt caaatgcata tagtaaacga atttcaatgt accgccagga gttaatgaag
    77581 gaatataatt cggatcaact gaaaccgtga atccgtctgt ttgctcagtg acagaataat
    77641 ttactgggtc gatttcttta tttccatcat ataaggcata agcatacgtt cctgaagaat
    77701 cattatcaaa tgttaaagca gcgtcatgag tatcgataag tttgaatgtt gtgtacttgt
    77761 tttgcgttcc ttctttatca gcgataccta atgggatatt gacagaaatc tcatattgaa
    77821 ttttttcacc gattgcgaca tcttttccat tcaattgagg tgttgtttta tcaactttag
    77881 acgtatcatt tttgatggtc ttttctactg gtgtttggct atttgccagg atctcaaaag
    77941 gcgtgattgt ttgcttttca atcatttccg cattatcagg agcttttact tcttctaaat
    78001 tatattttcc aacttctaaa tgattaacga tcaactggcc tttttcaata gatgcctcgg
    78061 caaagtcatt gttgccgatg tcataagaat gaccagtaat gaaatgtttg gctttggttt
    78121 gatcagttgt ccaagtgtac aatccatctg tgacactttg gatgtatttt ttgacgcttg
    78181 gtgttccttc ttctttagaa ataataaatt ctgctccatt tagtgcttcg ttttcggcag
    78241 taccgatttt tgtaactttc aacgttccat cattaccgac tgtattttta gggtagagat
    78301 ggatagtatc tagttcttct gtcccgtatt tataagagcc atctgcttgt ttgatcatct
    78361 catatacagg gaaagctaaa accatgtttg cggcagctga cactccgtct tttggttctt
    78421 ctttgatcgt gtagactgca tctttcccat tttgtttttt aggtaaagat aaagtgacat
    78481 ttccatcagc atctgtcgtt cctgaagcaa ctggtgtacc aggagtcaaa gactggactg
    78541 cttgttttgc tgcatccacg gacgctcctt tatctcgttg cgcataaaat tcttgagtga
    78601 cgttataaac tgaaaatgaa atatcggcta atccttggta ttgatcgaat tcgctcattt
    78661 ctttcccgct gttttggatt aaaggatcgg gtaaatcagt cattttcttt ttatgaagga
    78721 tgacttgagc tgctgtctcc tctgcaaaca ctttttttgc gccgaagctg tttgtgagta
    78781 atggtaaaat aaggaaaagg actcctaaca taacgtttat ttttttatga tttttcattt
    78841 tttcactcct gttctttttt tgataaacaa ccagattgca aagcttgcga ataccatacc
    78901 aacaacgcct aaaagtgtat ttttcgtttc gtttgttttt ggaagaaatg gacggctatt
    78961 ttctttttta ttgactactt tcaatatact gtccgcctct ttatcctcag aaagaaaagt
    79021 tgaaatggta aacggaattt ctgtttcact taaaatataa ccagaaggtg cttcgatctc
    79081 ttttaatcga taacttcctg ttttcaaccc ggaaattgaa aaagcgccat ttttatcgga
    79141 aatcaggtga agcgcatctg attcgttctt tgtccacttg tatccgttgg ctgtttgctg
    79201 gaggtaattt cctgctttgt ttttaataag gaatactgcg ccagctaatc gtttatctgg
    79261 tttatttgtc tcgattttga caaattgctt actccctgta tatacagaga ctttctttgt
    79321 actgacacca aaaccaaaat ccagtgttcc gttgttgttg atttccttgt tcgcctgtgc
    79381 tgtactgctt agctgcatct gataagaaat ggtcagtttt tttccagcat atttttccaa
    79441 cgaagggatc gaaaataaaa catcaaatcc gtgaggtttc ttgtgtatga caaagccttc
    79501 tgtcagcttt tctccattcg atgaaatcgt taaactatta ggtaaaaacg ttaatgcttc
    79561 atccgcacta tctgacaatt cgaacttttg atagtcaagg atatttacag gaattgtcgt
    79621 atgcaaggaa taagtgattt tttcaccaat cgtaaaatca tttctaggct cttctaatac
    79681 cttctcaaaa ggtggatcag ggtagtcgtt ttcttcattt ttaggataca aatggatagt
    79741 tgaaagtttt tgattgtttt gtccatgtac aggaagaaca actaccatat tctctgcctt
    79801 ttctttgacg acttctggta ctttggattc aatgaaaaga tacgctttat ctcttttttc
    79861 ttgatcttta gcggccagag aaaaagaagc gataccatct tcgttgttaa gagttgtagt
    79921 tgttgcttct gcggtaaaca taccggaaga cgcaccgttt tttgcgatct ctgcttgtgc
    79981 ttcttctacc gttttgccct tttcccgtag atggtaaaaa gaatctgtga catcataaac
    80041 ttggaatgtg acaccattta atcctcgata cgtttgtaat aaagcttttt cttgtccgtc
    80101 atttggatga tttttcggca gttgcccgtt tgggaaaagc aatttgtgta aagtaatctg
    80161 aaccatttct gtttctgttt ctgttgctac ctgagtaaaa gctggtttaa atagtaacaa
    80221 gaagagacac atactaagcc aaccaagttt tttcatattt agctgccccc tctttctttt
    80281 ttcaaaaaaa gataccaaat agaaaacgca caaccaatca tccctactag atagattcct
    80341 aaacggccaa ttcctcccgt ttcaggtaat ggtacttttg cctgattcgt gatgtctaaa
    80401 gaaatctggt tgtttttggc tcctggtgac agaacagatt catgatcttg tccatccact
    80461 tgaattgacc catcttcgtg tataactata gtaactggct cttttagacc ttggtatcct
    80521 tctggtgtaa aagtttcggt cagcgtataa gttccaggtt ttaaattttc gaatagaaag
    80581 gtatctgttt cttgtccatc ttttggcgat tctaagtcca tttctggtcc ctgcagtctg
    80641 aattttgctc cttttaatgt ctgtccgttg tcttcttttt tgtgtaccgt tagatcaaac
    80701 gcttttagtc gattttgatg agtgagcgtc catacattat ttgtcatctc ggtatttttt
    80761 cccgtgtaaa tgatttttcc atatttatcg atttggaatt cataatttcc cggcgaagta
    80821 tcatatccgg caggaccagc tgtttcaacg actcgatatt taccaggtgt gagtgaatca
    80881 aaactggcaa gacctttttc attcgttgtt tggctgtcaa ttggctgata agtaccattt
    80941 gcttcttttt tctgtaggga aaatgctgct cctttaagat ttatctcttt gtccgtccct
    81001 tgcatcgcat acttacgtac tgctacagga acttcaacaa aaggattttc aatagtcaac
    81061 tggatcgtat catcggaagt agtgactgtt tttccatcaa tggttaccgt accatcagaa
    81121 gcgatcttga tttcccaagt agtctttttg cttaatccat gcccttctgg agcttttgtt
    81181 tctgtcagcg tataggtcat ttctttttgc aatttgacat tttctggaag agaataggtt
    81241 ccgtcgccat gatctgttag taaagtatca atagaatctc ctgttaattt gaaaacagcg
    81301 ccaataagat ccttttcacc tgtagaggaa tttttcgtta ttttcaagtt taacggtgtg
    81361 aattgtttag tatttttcca tgtcattgca tctatttgtt gagaatcgta tcctggtaca
    81421 ggtaattctt tgattgtttg gtaactgaat gcttgacctt gattattgta ttgaggtagt
    81481 gataatatct cttgataact ttctccgcta tttgcagata atttgtcaat gtctgcacgt
    81541 tcccacgtat ttgttgtatc tttagctggt ttaatgattc gaatatatcc gtttttccaa
    81601 gctgcagcat ttgtcgtatg ttcccgttga atctcaaaag taacttgatc tggacgatca
    81661 gctagattgt tgtcaaattc ttcccataac tttttgatgt gaagactgac tccgggagct
    81721 ttagcagatg gtataccgaa ttcagctaat tcattggtat caatgtttgg ctggaaagtt
    81781 gtcctgccgt tcatttgata ccagaaattt ggatggaagt cctcattttc tgtttggatt
    81841 ctcacttggt aatggatttg gatttcttgg tcttttccta aatagatctg attgctcttg
    81901 atggtatttc cttctatgga aatagatgga gtgaccgttg taggacttgt ccccacactc
    81961 ttgactgaaa gtgtaccagg ctgataaatg aatggttctg cgattggatc atttatttgt
    82021 ccattgctta cagttgctga gatctgtaca gcttttttgg ctaggtattc agagatatct
    82081 gttgcatgat cagctgattc atagtaaaga tcgccttttt catctgatga aaccatttgt
    82141 cgcatacgag actctacttc tgcttttgag agaccagctg ccggatcgct ttgaagttgg
    82201 atgccaagac catgtatttc gattcctcgt tctttgagtg ccatcgcttc tccgatggtt
    82261 gcgataaacg tactgtcgat tcttctggat agattgtttt ggtcaggtgc atcatagatt
    82321 cttgagatta gagaagtatt tcccggccga tcttgcgtat tagaaaactg agttccgtaa
    82381 taattgctgc ttgattgtgc gtgtacccgc tgtactttat aggaaaatgt tggtacacca
    82441 tccgtcagca aaacgatcac ttttttatgt ccatttggaa cggatagcat gcttcctgca
    82501 tctcttagtg ctttttgtgt aaaagtacca ccatttgtcc gtgaaggtgt aatggatttt
    82561 acttgatttt tcactgaatc aaatgaaccc atctgtactg caccgttact gtagctatat
    82621 ccttcgcttg agtagccgac atatcccata ttgattttgt ctgtgatacc gctatctgct
    82681 aaagtatcga caaaacgatc gacaccaatc tttacttcac cgatccgatt attgtcgttc
    82741 atacttcctg accagtctac gaccaatacg agatcaagag gcgtgatatc tttttgtaca
    82801 tttccacgag cattcaaata aacatcaaac aatccgggtg tacttgtttc ctttgcatac
    82861 tttcgtatcg catagtctgc ttgattaccg gttcctccat attcgatata cgaatgggtc
    82921 cgatcattag gatctccatt ccaagaattt ataccatccc attgatttgt tccgttttta
    82981 ttgccttgat gatcaagaac atttgtattt cctgtaggtt gccaactggc tttaggataa
    83041 gtgccctgat catctgttgt atattctgta ggaatcgttt gattagatgg cacactcaca
    83101 gctggcaagg ataatgtagg caatgtaaat gggctgacag aattttcgtt ttcttttgat
    83161 gattcttctt ctatttcgtc tggctttggt actgacacat ctgtatcttt cttattttct
    83221 gtctcatcaa taatgtcata ttctttatta tcgacgatca gcttgtttgc ctggctgtgg
    83281 taaagtgctg gatcgattgg aaaactcaga tcgatagttt gtttagtgtt tgatgagatt
    83341 tccaacgtaa ttttcccgct atttacctga taagcatagc catctccttc ttttctttcc
    83401 acatcactta agtaattttc gactagacca atttcttttt ctatcttttc agcactggtg
    83461 ttattcgcat ttagcttgat gttcagtgct ttttttccag actgatccga aatgacttca
    83521 gctgaaattg tttctaaaac tgttggatca tctgtagtgt ctgctattgc agataatggc
    83581 acaaagtttg ataaactcaa tattatcagc atgaaaataa ctttcagttt cttccctgtg
    83641 gtcgtcattt ttcccctcct ttttcttttt ttctgttttg acgtgaatag tgaaaattat
    83701 taaaataaac aggcgttaca taattttatt tattttaata tgataattat tatacgaagg
    83761 gcgctaaagg gaatcacacc aattttcata ccaattggtt tcatttatga agttgattcc
    83821 ttcgattaat gagcgtttca ttgaaagaaa agggttgagg gagaaaaaca tatgatcagg
    83881 ttgttggaat caaactatag aaagatggaa aaaatcattc gaattctgtc catttctgac
    83941 aagtggatga ctaaaaaaga acttgccaaa cgaattggta gttcagaatc tacatttgta
    84001 aggtatatcg aagagatcaa aatccgctgg ggaacggatg taacgattca atcttcacaa
    84061 aaattggggt atcgtcttga acatttgaat gtaccagctt atttaaatgt tctgatggaa
    84121 atgtcgcaaa gttctacaaa cagtcgttta ttaaatgaga ttattgtcaa tccgggtaaa
    84181 acgattgagt attattgtga aaaaatttcc atcagccggt cttcttttac acgtaagttg
    84241 aaacaatgca atcaaatttt agaaagctat ttattaaaag tgatcgttga ccaaggtttt
    84301 cagttaacta gccttaaaag tgagctccag ttgcgtatct ttgttacctt tttcttttta
    84361 agttactacg gaagacgcac gttgccattt ccaatcgaaa aaaagaagat caaagaaatt
    84421 atggtcaaga ataattgtca gatgaatttg tttcaggaag aaagcccata tgagcaatct
    84481 ttctttatta tgtattttgt tgtgcatctc atgagagaat cccaaggaac tccatttgaa
    84541 tggaaagctg gagactctta tgtacagttg ataaatatcg ataaaaaaga cttcttaatc
    84601 ttgaaaaatc tcttaggaaa attgaagtac agcagctata taaaagtagt gaactatttt
    84661 gtcaatacga ttttttcaga attttcggag aaagaaaaaa atgaaatcag tgagcagata
    84721 cgaaaaaata tgtcctactg ttcgttttca tccgctgatt gcaaagaaaa aaacgaagaa
    84781 tatgttgttc aattattaac gcaaatttat tttttctccc gtattttccc atacgatacg
    84841 actcatttga cgatccgtgc aacttacttt gcacagcaga tgcaacaccg ccagatacgt
    84901 ctatatgctg gatttcgtgc agaacttcaa ttttattcca ctctactgaa tcagaatctg
    84961 atttattatt tatctagcta tatcttttgg atgcttatcg gtaatcctga aattcaccat
    85021 tatacttctg ataaaaaaat actgattatc agcgacttaa gtcttcgaca tagtcaatat
    85081 attgaagaat atatcagtga tattcttgct gtgcataaaa ttcactctga gacaactgct
    85141 attactgaag atcagctgtc gaaatacaat cttctcgagt atgatctgat cgttacgaat
    85201 caacccattt tgaatgccca tgttcctcat atattgattg atgatagtgt gtcattcgca
    85261 aatgaagaag aactcatccg cttattcgag ttatgaataa tcaaaaaaca gctatgtata
    85321 aaccaccctg ataaaaattt caggacaaat acacatagct gtttcttttt attgtattcc
    85381 ttcagcaatc caacgagatt tatagcgaaa gtcttttaac aactcttctt ttgtccaatt
    85441 atttgcaatg agatccgtca aagtgttcat tacgagggtc atggaaaagt ataggaaact
    85501 aactgcttct tccgacgatt gaatggagaa atgattgatt cttaataatt ccagccattt
    85561 ttcgttcatt ggagaatcag ccgatagttc tctacgtttg gttgtctgct gatcatttcg
    85621 ctgggttaac aagtataacc tttgccaata gatactttcg tgatctaagt cgcagcacca
    85681 ctctaaatat ttttcgatcc ccaaaaagaa atgttgtttg ttttcgctaa tgaattttaa
    85741 gatatcttga tggactatgt gagaactgct tttgatcgta tacaaataag catcttccaa
    85801 atcttgaaaa tatttataaa aagcaccgcg cgacatcccc atcttgtcaa cgatttctga
    85861 gactttgact tggctgatgt gctgtgagca aaatatctcc agtaataggt catcgattct
    85921 ctgtttcttt gcgttcgata aattgaaaaa agtttctttt ggcatccaca tctctccttt
    85981 atctctgatt atacgtggat agcccttcat tgtcatgaag gattcattcc ttctttttct
    86041 cttcttctgt caagtaccca tcttccattc gataaacgcg atcactccat tgaatcatac
    86101 ggacatcatg agtaaccata atagtcgctt tttgtttctc atgagcttct ttaactagta
    86161 atttgacgac ttcatatgca tgatctgtat ccaaactagc tgttggttcg tctgctaaga
    86221 tcaagcttgg ctcattatat aaagcgcgag caattgccac tctctgtctt tctccaccag
    86281 aaaggtcttt aggataacta tttttaagtg actggatatc taaagattgc agcaaggaat
    86341 cgatttttgt tgaatcttct ttttttgaag taacttggtc aactagttca aattgttcct
    86401 ttaccgttaa aaaaggaatc aaattagagc tttggagaat aaatcctatt tctttaaagc
    86461 gtaaatctgc tcgttttttt tctggaagct cagttagtga gtgaccgttg atcactattt
    86521 ctccggaagt cggtgtttgc aatcctcctg aaatggtaag gaaagtactt ttacctgaac
    86581 cagaaggacc aatgatgctg acgaattctc cttcctttac ataaaaatca attgctttca
    86641 aagctgagac ctttgtatga ccagaaccaa atgttttggt aacttttttc atcgttaata
    86701 tttttttcat ttttattaac ctcctattgc tgtaattggg tctactttgg ttatcgtacg
    86761 tatcgaaaac aatccgccta gcattgcgac aatgatcaaa atcccactat atagcaacca
    86821 ttgtgaccaa aaaacagcaa aaggcatcgc gcttggtaaa atcagactag tcaaataagc
    86881 aaacaaaaga gcaagcagaa cgcctaccac cccaacaata aaggactgtg ctactaaaga
    86941 tttagcgatg aagaaatttt tgatcccttg tgctttcatt actccaaaaa tagcagtctt
    87001 ctgaagtgtc atcacgtaca tgaaaatacc gaccacggca gcaaccacta agaacaaaaa
    87061 gtatatcatc gcatttaagg tcatattttg ggcagaatag ccaggaatac tttcaatgaa
    87121 tgtcccaata gttaataact gtagaccctt cgatgctttt tctttcgaaa ttttagctga
    87181 atcctttatt acgatcccat tgatcggctg ttcttcttca gaagcaaacg gctgttgtcc
    87241 atacttcaaa gcagtccacg tatctaggga cgcatagatg acgggagaaa ctgtgtaata
    87301 agtttcagga aagataccga caatcgtcaa aggatcgtcg taactgccaa ttttgatcgt
    87361 atctccaatt ttatagcctg aatcagccag attttgagag atgatgattt cattctttgt
    87421 tttaaattca tgccctttcg tgagttttgg taataaaaat gcttctttcg ttgtgccgaa
    87481 aactgtaaca ttttctttgt tttttccttt gattgctccg ctgtacaatc cgattggtgc
    87541 tttctctcct ccttcaatgt atttcagatc tcctcttgtt aattgtgaag ctgcaaatac
    87601 ttgatttgca tcctccgaca aaacaatctc ttgtgcgtcc caatcatcga ttgctttttt
    87661 aaactcttct gaaagtccat tcgctagtcc tgacaacatg aatacgacat acgcaatcaa
    87721 gaacataata ccgacgatca agccatatcg taatttagaa tagcgaattt ctttgatagc
    87781 tagaaacatt tgtttttcct ccctatgata gattgatagt gacaaaatgt cacttgttga
    87841 atagtgtaac tctaaagtga caatttgtca cttgtgttgt ttttttctta tcaataaatt
    87901 gtctaagaca tctttcgata aaaccttaac atgagtcata aaaaatttat ttaagcctta
    87961 tttatgcttt ccatgataaa ctaaagtgac caaatcaatg aggtgggaaa atgattaaac
    88021 tattagtagt agaagacgat aaagtattat ccaataatat aaaagaaata ctcgctgcaa
    88081 tcggcgagat aacacaagtt tatgatggta gtgaaggact gttcgagttg gaaagtggag
    88141 tttatgatct tgcaatcttg gatttgatga tgccggaaat gaatggatat gaagtgttag
    88201 caaaaatacg gaaagaaaaa atacagactc cagtattgat tttgactgca aaagatagtt
    88261 tggaagataa gattgaagga tttgaacgag gtgcagatga ttatctaaca aaacctttct
    88321 atcgagaaga actgatcatg cgcgtgaaag cattattgaa acgctcgcta ggtctctttg
    88381 atgaaaatgt tctgaagtat aaggatatca gttgtgattt aaaaagcaat gaagtcttgt
    88441 ataaagaaca agttttgccc atacaaggaa aagagtttga attgctcgtt tattttttac
    88501 aaaataaggg aatcattttg acaaaagagc agatatttga ccggatctgg ggatttgatt
    88561 ctgatacgac gattacagtt attgaagttt atatgagcaa cttacgaaaa catctgcgaa
    88621 gtactggatt ggataaagaa atcaaaacct tacgtaatgt cggttatata ctgcaaaggg
    88681 tgtgatgcaa tgaatggaga tattacacga aagcaacgta gtcgcttttt tgcgacaaat
    88741 gtagcagcat ttgcttttat cttttttctt ttgggattca ttactttaca gatattaaac
    88801 caatctgcat acagagaaac agatctgtca ttagagaaaa taaattttga ttctcgaaca
    88861 gtacaattag agatacaaag atatcagcaa ggcgatccct ttttaaaccc gactcaaacg
    88921 gaacaaaaca gaacaaacat ggaacagcca ttaatggaac catcaaatga ccgttttaac
    88981 actcaagtga tcttgtggtc taaaaatggt gagatattga ataaagcagc tattggtggg
    89041 cgtttcaacc aaatcaaaga attaaaaatg gatacgaaaa atctgaatac catccaaaat
    89101 attgaattaa atgaagacag tggctcatct gccctctctt tccgttcgat tacgaaaaaa
    89161 gcggagacga atcaagatgg cgttgcctac atccaaattt tagaaaatac aaatcaaatc
    89221 aagaattcat tagcgacttt tcgcacgatt atgattttgt gtatgattgt tttttggatt
    89281 ctctctattg gtatcagtta ttacttgtct agtctaagca tgcgccctat tattctttct
    89341 tggagaagac aaaaagaatt tgttgagaat gcttctcatg aattgcgcac accgttaacg
    89401 attattcaaa atagtttgga acatcttttt accaagccta atcatactat tattgaagaa
    89461 tccgaaagca tcgctcaagc attgtcagag acgaggcgcc tcactggatt gacttcggat
    89521 ttgctgacaa tcgctcggaa tgattctgat cagcagctgc tttcaaagca aatgatcaat
    89581 acacaagaat atatcgaaca acttgttaaa ccatttcaag agatggccat catagatgga
    89641 aaagagttta ttctccataa cgatgcaaca ttagaagtac caattgatga aaaaaaaatc
    89701 catcaagttt tggttatttt gctggataat gcattaaaat atacgaatcc agaagataag
    89761 atcattttgg attcatctgt taaccataaa gaatggctga ttcacgtaaa gaatactggc
    89821 ccttctatca gtgaagaaga tcaagcaagg atttttgatc gattttatcg ggaagatcaa
    89881 tctcgggcga aagagactgg cggatacggg cttggcttgg caattgcaaa acaggtaatc
    89941 gaacagcatc atggacagat ttctgtatcc aattatcttc cacaaggagt cgattttaaa
    90001 gtccaattgc cgttgaagcc taaaaaataa tgcttatccc taaaaaaggg ctgtgacaaa
    90061 aatcttgtca cagccctttt ttgttgcgaa taaacggtgt tccagaagta gcttcttcgg
    90121 aaataagccg aaatttcttt aagctgacca cttctgtcac aacctcttta gcttacgctt
    90181 acgatttttt attgcggtct tcaatgtgta catcaccgct tgtgtcaata tccaattctt
    90241 ctcggctaac atcttctgtc actttttcaa catcttcatg ttcttcttta tgaatcccaa
    90301 cttcttcacg aataactgta tgtttgctta catctacttg ctcttctttg attgggatag
    90361 taatcgtgtc atcttccaat ttttcgtctg ttccctgtga agattgatct gttactggtt
    90421 tgcgttcgat gacgatttct tcacgtttga ccggtacttc taccgtttca gtatcgttga
    90481 ccacatgttt gtggatgtcg acttctccag ttgtcacatc atgtgtattt acatctaatt
    90541 tttcttcttt tagttgaatt gtatcatcat ctagatttgg tttgcgatcg atgtcttcag
    90601 tcttgcttgt acccattgta tcagtctcgc caggtacgtc catcggatca gttcctggtg
    90661 tcatattcaa tggatccgtt gtattttgaa agcctgtcgt gccacctacg tccattggat
    90721 ctgttccagt gccagtttct aatccagtcc ccattgtgct tgctgggcta acagtatctt
    90781 gtgtatccat tgtatcttct gttgtttctg gctgacgata gccttttact gtgatgacaa
    90841 attttccatc agaaatatct cctctatatt gatataaagg atcattttct ggtgtagccg
    90901 tctcagaatc ataggtatca aaagaaaaag tatcttttat tttttcccat aaagaacggt
    90961 cttcttcacc atccatccga ttttcatctg tttcaacatc tataccagac aatccgtcaa
    91021 atcctagtct agatgctgct tcatcagtag tataaataac gatatcatct cgttcatacc
    91081 cttcgttacg caaccgttca attactgata atgcttcttc cctcgtacta tagcttccga
    91141 tgaccgttaa atttttctct tccatgtaaa ttcctcctag caaatagatt tatgattggt
    91201 atattgttac ttataaattt attctatagg agacgattgt tttctacaaa tatttagatt
    91261 ggaatttgat taaaacaatt taattgaaat ttgaaaacat ttatgttaat gaaagtcata
    91321 ttctaataaa atgtcatttg tttctatatg aatttttatg aaaaatttca tataatttcc
    91381 actcgttgtg atggtttaat ttatccaatc catcaataag caaacaactt atataaaaaa
    91441 ggattaattc tcaatataat tgctcctacc atagaataaa tcttcatgta aagtaatgaa
    91501 gtaccaacta tttctaatac ttctttaaga attatcccaa caatatcgtt agacaaacca
    91561 ttattgaata aatggagagc caatcgattc tgaaaaaatg ttttctaatt tttcggaaat
    91621 aaaagaaaga gcctcctgcc attagaacga accaaatcca tttcattgct catgaagtaa
    91681 ccacatagct aaaaagagga taaatcaatt aaaagatcaa aagaatgact gcactaataa
    91741 aataactaaa aatgtatctt cttacttcat atcttttagc tagtacttag ttaaaaattt
    91801 gcttcctaag atcaagacaa actgtctatc ttcatttgat gaaatgcggt aaaatataga
    91861 aaatgaacac tattggaggg aatttgtaat ggacagcaaa actagaaaac aactgcaaga
    91921 aaagttaaag caacgtacac acttgctccc ttccgtcttt caacagtttt tagaagaaaa
    91981 tcgttcttcc ctttcattag gcagtcgcta tgaatacgca aaagattttc atctgtttac
    92041 tgattatttg agaaaagtat tttctgaaga agtggatgat cagctgatta tccatttaga
    92101 aaaacaagat tatcagaatt tcttagctaa aatctatcga catactcgtt cgttccaaac
    92161 tacagcgaac caagaaacgg agcaattgtt tgaaaatagc tattacggga tttcaaggaa
    92221 gcaatattca ttaaatcatt ttttacgatt tttgtatcaa aaaggactga ttgaagaaga
    92281 gatttctctt ttgggtacaa gtttaccgga aacaaccaaa gcagctccgc gatatatcga
    92341 tgcagaactt ttcaaagatt ttgagcattt gtttatcccg attgaaggct ttcaaacaag
    92401 cagagaagct agctttgaag aaaagaatag accgaggaac cttgcaatta ctgccattct
    92461 tctgtattgc ggattaaaga tccatgaagt agtagaactt aaacgtaaag atgtatcttt
    92521 gaccaagcag attcttaatc tgaatagaaa agaaaacaga ctgaataaag taccaatacc
    92581 aaaagaagcg atgccatttc tggagaacta ttttaagtta acagcacaaa atacggacga
    92641 aaattctttt gtttttcgtt cctcacatga tcagcaaatt tctcctcgga ctattcgcca
    92701 aattttgagc aaaattacag tatataaaaa cgtatcgcca gaactatgca gaaaaacata
    92761 tcgttattat gcagaactat atttagatga tgctgacgcg gtaaattatt tatgtggtaa
    92821 tcgatacctc tcatcccatt cttttcaaga tatctgggaa aagatggttg atttttctta
    92881 tcacgaatta gcggcattcg ttcgggtttc ttccatataa aacaaatgta tgatcttttt
    92941 ttcacaataa actatgttat tatataaagg attatgttac gattatcttg aatttgtata
    93001 ttacttaaca aaagataatg gagtgtgaat gatggagacc ataacgtatt cccctttgaa
    93061 cttatttacg aattttcaag aagctgcagc taacttttcc gagaccccga ttatctttga
    93121 tgagccactt tcagctttcc cagaattagg acttgaaaca acttacaaaa aatgctgcga
    93181 aagtgtgttg aaacgggctt atcaattggc tcatcttgga gtgaaatccg gagacaaagt
    93241 tattatttat aagagttccg cttttgacac ataccttcta gctgtttctg tgtcttacct
    93301 tggtgcagtg cctgtcatga cttcttatca tctaccaaca acaacaatgg aagtatttat
    93361 cgatcgttta gaagaccctt tcattttatt tgatgatgag acaaaaaagc gagtgagaga
    93421 aatttcaaat ggaacaaaat caaaacagat tcc
//
LOCUS       DACGLE010000004        77984 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0534
            SAMEA3304449-rid9560453.denovo.004, whole genome shotgun sequence.
ACCESSION   DACGLE010000004 DACGLE010000000
VERSION     DACGLE010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304449
            Sequence Read Archive: ERR985064
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 77984)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 77984)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702225.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702225.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 20:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,045
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 27 of 67
            Pseudo Genes (incomplete)         :: 25 of 67
            Pseudo Genes (internal stop)      :: 19 of 67
            Pseudo Genes (multiple problems)  :: 4 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..77984
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304449-rid9560453.denovo.004"
                     /strain="VREN0534"
                     /db_xref="taxon:1352"
     gene            <1..171
                     /locus_tag="IXT20_000332"
     CDS             <1..171
                     /locus_tag="IXT20_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HAQ4858809.1"
                     /translation="SLFPLYHGSAKSNIGIDNLIEVITNKFYSSTHRGPSELCGNVFK
                     IEYTKKRQRLAY"
     gene            346..642
                     /locus_tag="IXT20_000333"
     CDS             346..642
                     /locus_tag="IXT20_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4858810.1"
                     /translation="MQIGVLKWLQFTKQKGVHFPMQFLEPKNKNAKSVDWEISEQVRV
                     IVKQYAEYAERTESEAVDEFLLNILDDKKFIEWIANKRSNKRIVEKMGIKDRVG"
     gene            649..2601
                     /locus_tag="IXT20_000334"
     CDS             649..2601
                     /locus_tag="IXT20_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insertion element protein"
                     /protein_id="HAQ4858811.1"
                     /translation="MTKLKRLATKEDEIVDVKIPISNEELKDRAKQYDLLTPKRFATR
                     YNKMLFLPTSFKWNGSEYPIQYNYCINPFCCNFGKEQHKFKDVKGKPSRYKMTGSSKD
                     KGHKGMYCNDNPIGRGVSQNCTVTPLSNWSVVEEIKRLIEINSIQDVEPDYQFHKEGC
                     SEEESTPFNEPKQFYKRGKSRGKSQRYQCKACKKFTNVLPKREETTTYHQQKNTILPM
                     FAKMVVGRVSVSRTCDILGIGVGTYYHKLEWLYRRCLEFLERYETQPLQTKKFNEMWL
                     NTDKMHYYLNNVRKKGQGSKKYTGFEDLNMQTYIVVSAEVLSRYVFRSDVAYDWNISM
                     DELNEDTRKFKEDHLNTFSRKNDRLDWSYYPQEPSANDSENRNAYLHELGKITNRSRF
                     VDGLNVDAPYTTTAHYWLIKQMVNADEWRMISDDDFSIRNAFYRVFTKELRLSDAHHF
                     ICQVNKTKSRKQCLKEFGQAKAELLDWGDIRGFKTKFLRTLASHYLTELLTSHQFHEE
                     AISKDGERYRKYADNPIKHPLATKDKGFYSVDCRTDLSALEPNEIAKMLLNVNDHSTN
                     SFIQQIRRYISSLERPLTTARGDKKSYIYANFNPKYAQFAITILRTYYNFCRPFKSAD
                     KKVLTPAQRLGITDKQFDWKDIIYFK"
     gene            complement(2673..3170)
                     /gene="dfrG"
                     /locus_tag="IXT20_000335"
     CDS             complement(2673..3170)
                     /gene="dfrG"
                     /locus_tag="IXT20_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HAQ4858812.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
     gene            3532..4572
                     /locus_tag="IXT20_000336"
     CDS             3532..4572
                     /locus_tag="IXT20_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000288739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HAQ4858813.1"
                     /translation="MYTSINGELCKIDRAYSGEIVILQNEFLKLNSVLGDTKLLPQRK
                     KIENPHPLLQTTVEPSKPEQREMLLDALLEISDSDPLLRYYVDSTTHEIILSFLGKVQ
                     MEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVSPLP
                     LGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYSPVS
                     TPADFRMLTPIVLEQAFRKAGTELLEPYLSFKVYAPQEYLSRAYNDAPKYCANIVNTQ
                     LKNNEVIIIGEIPARCIQDYRNDLTFFTNGLSVCLAELKGYQVTTGEPVCQTRRLNSR
                     IDKVRYMFNKIT"
     gene            4670..4858
                     /locus_tag="IXT20_000337"
     CDS             4670..4858
                     /locus_tag="IXT20_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011988843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAQ4858814.1"
                     /translation="MINYKWILCPICGNKTRLRIRVDTILKNFPLYCPKCKNETLINV
                     QKMNIITIKEPDAKTQSR"
     gene            complement(4916..5269)
                     /locus_tag="IXT20_000338"
     CDS             complement(4916..5269)
                     /locus_tag="IXT20_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ4858815.1"
                     /translation="MRKKEDKYDFRAVGLAIKEARMKRGLTREQVGTMIEIDPRYLTN
                     IENKGQHPSTQVLYDLVSLLHVSIDEFFLPTDNLIKSTRRLQVEKYMDSFTDKELSLM
                     EALAKGINEARNIEG"
     gene            5800..6222
                     /locus_tag="IXT20_000339"
     CDS             5800..6222
                     /locus_tag="IXT20_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000804881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAQ4858816.1"
                     /translation="MKPSSFQETIENQFDYICKQVIEDERKDYFKHLDRLSAKEVSFS
                     DIDNYLVNSFSTVDTYVTDFQLFKLFGLRINVESDLLSEALRHLSEKKRNIILLHYFM
                     DMSDVEIAELLNLNRSTVYRHRISGLAMIKEFMKECKE"
     gene            6219..6449
                     /locus_tag="IXT20_000340"
     CDS             6219..6449
                     /locus_tag="IXT20_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ4858817.1"
                     /translation="MKTAYPRVPFPLIVKATDGDVEAINQIVKHYRGYTSKRSLRRMT
                     DEYGNSHMVIDETLRGRMETKLISKILSFEIK"
     gene            6947..7147
                     /locus_tag="IXT20_000341"
     CDS             6947..7147
                     /locus_tag="IXT20_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008475635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HAQ4858818.1"
                     /translation="MITVTKKDLVELGYGTSFSADIIKKAKELMITKGHIYYQSRKLD
                     RVPREAVEEILGIRFSNENRSE"
     gene            7174..8367
                     /locus_tag="IXT20_000342"
     CDS             7174..8367
                     /locus_tag="IXT20_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAQ4858819.1"
                     /translation="MVKDPIKKAKNGTYYFRANLGYDSKGKKIQKYRSGFTTKKEARE
                     EYSKLLLMKPEELSENKDKMTFKHYIFEIFLPWYKTQVKLRTYENRLPTIKKHFSYFD
                     KMAVSDIEPIDVQNWQLKLSKKCKSSYVRAVQGLFSVAMDRAIVLGITTTNPSKIIGN
                     VKKQKSKIEFWTKEEFEKVISLIYKEDYYQHFLFISLWFLFMTGMRIGEATAIQWEDI
                     DFDSGVLTINKTLYYKNQSNYRFVEPKTKASVRHIVLDKCTLTYLSEWKEVQQNLIQT
                     DFVMSYNGIPTQKHTLSYAIERYAKLAGIHRIRIHGLRHSHASLLISMGENPLIIKDR
                     LGHEDIETTLGTYGHLYPNSNFEVANKLNGAISFKEAKQNYDTSPKNQFTVDYLRNKE
                     MKKVQ"
     gene            complement(8435..10000)
                     /gene="guaA"
                     /locus_tag="IXT20_000343"
     CDS             complement(8435..10000)
                     /gene="guaA"
                     /locus_tag="IXT20_000343"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HAQ4858820.1"
                     /translation="MTNVADMTNVEKIIVLDYGSQYNQLITRRIRDLGVFSELLSHRI
                     TAEEVKAMNPKGIILSGGPNSVYDENAFGIDPEIFNLGIPVLGICYGMQLITYKLGGS
                     VEPAKNREYGKALLEVTSDQAVLFKETPKQQTVWMSHGDLVTKVPEGFETVGTSKDCP
                     IASIQDTTRNFYGIQFHAEVRHSEYGIELLRHFAFDVCHCEGNWTMDNFIDMQVEKIR
                     EQVGDKKVLLGLSGGVDSSVVGVLLQKAIGDQLTSIFVDHGLLRKGEAEQVMESLGGK
                     FGLNIIKVDARDRFLDKLKGVSDPEQKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQG
                     TLYTDVIESGTETAQTIKSHHNVGGLPEDMQFELIEPLNTLFKDEVRELGTQLGMPDS
                     IVWRQPFPGPGLGIRVLGEITEDKLEIVRESDAILREEIAAAGLDRDIWQYFTVLPGI
                     RSVGVMGDGRTYDYTVGIRAVTSIDGMTADFARIPWEVLQKISVRIVNEVAHVNRIVY
                     DITSKPPATVEWE"
     gene            complement(10059..10982)
                     /locus_tag="IXT20_000344"
     CDS             complement(10059..10982)
                     /locus_tag="IXT20_000344"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HAQ4858821.1"
                     /translation="MNDPMNYYRVAREEWREFYRNGQAPLTQDELDNIKSLNDRISMQ
                     DVRDIYVPLAHLIYLYMKEFESLFLSKGLFLHKYVEVPPFIIGIAGSVAVGKSTTARL
                     LQTILSRTFKRRNVQLITTDGFLYPNRILKERGIMDRKGFPESYDMEMLIDFLNRVKS
                     GQEEIKVPVYSHDVYDIIPDKYELIQQPDILIVEGINTLQLPANQQIYVSDFFDFSVY
                     VDADPVLIEKWYLDRFGALLDTAFQNPENYYYRFAIGDREEAFKMAKDVWKNVNLKNL
                     QEYILPTRGRADIILHKTENHIIDSIFMRKY"
     gene            11178..11783
                     /locus_tag="IXT20_000345"
     CDS             11178..11783
                     /locus_tag="IXT20_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAQ4858822.1"
                     /translation="MTNHYYSERPDTAHDFEQWAFELKGRTFQFVTDSGVFSRETVDY
                     GSRVLIDAFDWEELPDEGKILDVGCGYGPIGLALAFATQRFVEMVDINERAVSLAQGN
                     AKRNQIEHVDIHQSNIYEAVHEETYAAIVSNPPIRAGKKVVHEILTGAYPRLKKGGTL
                     TIVIQKKQGAPSAQKKMEETFGNAEIVTKDKGYYIIKSVKE"
     gene            12091..13107
                     /locus_tag="IXT20_000346"
     CDS             12091..13107
                     /locus_tag="IXT20_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAQ4858823.1"
                     /translation="MKLTIRDIAEMAGVSVTTVSQILNNKGSRFSDKTRKKVLSIVNE
                     HHYKPDYFASNVINRHSKTIGMIVPDVTDFFFSKLIEGVESYLNPLGYVIMLCNSRHS
                     QENEIKYLQELSHRSVDGILLATPNILPEKYALDSGFYQKMPIILIDRGLNRRDNGRL
                     IVKEYEGAYQAVSLFIENGHTKIGMLKETTGYYQLEERFNGYRHALKDHGLPFNGKFV
                     EHGELTVQGGYTASKKLLKHKDITAIFCGNDAMAIGCYQAIDELGKKIPEDISVIGFD
                     GLKLSEYMIPQLTTVQQPSFDIGFYAARFLIDTIEFPQRKVPNKVFETKLIIRESVKV
                     LTKG"
     gene            13248..14549
                     /locus_tag="IXT20_000347"
     CDS             13248..14549
                     /locus_tag="IXT20_000347"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HAQ4858824.1"
                     /translation="MRMVDLIEKKRDGNELSKEEIEYIVTNYTNGKIPDYQVSALLMA
                     IFYQDMTNEEITNLTLAIANSGDVIDLSSLEGIKVDKHSTGGVGDTTTLILAPLVASV
                     GVTVAKMSGRGLGYTGGTLDKLEAIPGFQIELSDEEFVRIVNESKVAVIGQSGNLAPA
                     DKKLYALRDVTATVDSLPLIASSIMSKKIAAGADAIVLDVTTGDGAFMKNIEDARRLA
                     KTMTSIGKLANRETVAVISDMSEPLGEAIGNSLEVVEAIETLQGNGPEDLVEMCYALG
                     SQMVVLAGKAKTTDEARTLLQEALESGKALAKFKEMIQNQGGDPAIVEHPERILTARY
                     TMELPAKQSGVVSKIVANELGIAAMMLGAGRKTKEEDIDHAVGLKLHKKIGDTVTKGE
                     SLLTIYSNDEEITSVIELLYKNIEIGESAMKPTLIHDIITE"
     gene            14595..15257
                     /gene="deoC"
                     /locus_tag="IXT20_000348"
     CDS             14595..15257
                     /gene="deoC"
                     /locus_tag="IXT20_000348"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HAQ4858825.1"
                     /translation="MELNRMIDHTILKADASKEAVMQIIEEAKKYHFYSVCINPAWVA
                     LAKEQLQGEPVAVCTVIGFPLGANTPETKAFETTDAINNGADEVDMVINIGALKSKDD
                     QQVQKDIEAVVEAAKDRALVKVIIETSLLDREEIIRACEIAKAAGADFVKTSTGFSTG
                     GAKVEDVRLMRETVGPEMGVKASGGIHNEEEAMAMIEAGATRIGTSAGVAIVSGSTGE
                     GY"
     gene            15278..15676
                     /locus_tag="IXT20_000349"
     CDS             15278..15676
                     /locus_tag="IXT20_000349"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HAQ4858826.1"
                     /translation="MAELKQEWINTAIEALDKAYVPYSHFPVGACLVTESGKIYQGIN
                     IENASFGLTNCAERTAFFKAVSEGEKTFTHLVVAGHTPDPISPCGACRQVMAEFCSPD
                     MPVTLVGDNGVTKATTVRALLPYAFTEKDL"
     gene            15742..16824
                     /locus_tag="IXT20_000350"
     CDS             15742..16824
                     /locus_tag="IXT20_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HAQ4858827.1"
                     /translation="MKKAKLFGLGAVALAAGLFLGACGNNGSTDSSGGKSSSDTTTAA
                     LITDTGGVDDRSFNQSAWEGLEKWGKDQGLSRGNDGFQYFQSSNESDYIPNIDQALNA
                     GFKTIFGIGYKLKPAIQEQATNNTGTNFVIIDDVIDGLDNVVSATFKDNEASYLAGVA
                     AAYTTETNVVGFIGGVKGEVIDRFDAGFKAGVDAGAKELGKEIKVLNQYAGDFSAPDK
                     GRSIAQGMYAQNADIIFHASGGTGNGVFQEAKSLNESGDKKVWVIGVDRDQSDEGEYT
                     LNGEKKNFTLTSTLKAVGTVVEDLAQKSADGKFPGGEHTVYGLKEDGVGLTEGQLSDE
                     AKKAVDEAKEKIISGDVKVPETPEEN"
     gene            17036..18598
                     /locus_tag="IXT20_000351"
     CDS             17036..18598
                     /locus_tag="IXT20_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ4858828.1"
                     /translation="MVQENIVIEMQNITKEFGTFKANDNINLQVKAGEIHALLGENGA
                     GKSTLMNVLSGLLEPTSGKILMRGKEVKITSPTKANQLGIGMVHQHFMLVDAFTVTEN
                     IVLGSEPSRAGMLDHKKARKEIQKVSEQYGLSVNPDAYVRDISVGMEQRVEILKTLYR
                     GADVLIFDEPTAVLTPQEIDELIVIMKELVKEGKSIILITHKLDEIKAVADRCTVIRR
                     GKGIGTVNVKDVTSQQLADMMVGRTVSFKTMKKEAKPQEVVLSIENLVVKENRGLEAV
                     KNLNLEVRAGEVLGIAGIDGNGQSELIQALTGLRKAESGHIKLKGEDITNKKPRKITE
                     HGVGHVPEDRHKYGLVLDMTLSENIALQTYHQKPYSKNGMLNYSVMNEHARELIEEYD
                     VRTTNELVPAKALSGGNQQKAIIAREVDRDPDLLIVANPTRGLDVGAIEFIHKRLIEQ
                     RDKYKAVLLISFELEEILNVSDRIAVIHEGEIVGIVDPKETSENELGLLMAGYTLEEA
                     RKELEKGAGAHE"
     gene            18591..19724
                     /locus_tag="IXT20_000352"
     CDS             18591..19724
                     /locus_tag="IXT20_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ4858829.1"
                     /translation="MNDRSEKFRNLLVPVLSVVLGLILGAILMAAFGFQPIQGYSSML
                     DAALGSQRGIGETLRQATPLIFTALGFSVANSAGFFNIGLSGQALCGWIVSVWVALAF
                     PDLPKVVLLPLCVILGALAGAMAAAVPGLLRAFFGTSEVIVTIMMNYILLYVSTFALH
                     EVMPESYRSSLDSSNMISQNASLRLPWLTQAFGGSRVNSGLFLAIIALIFVWLLMKKT
                     TLGFEIRSVGLNPFASEYAGMSSKRTIVLSMVISGALAGLGGVVEGLGTYQNFFVQTT
                     SLSIGFDGMAVSLLGSGSAIGILLSAILFSILKIGGLGMQTGAGVPFEIVNVSIALII
                     FFVGINFLIRFVMAKVFQGKKQEEIVATIESKPTNQNHEGGEL"
     gene            19724..20680
                     /locus_tag="IXT20_000353"
     CDS             19724..20680
                     /locus_tag="IXT20_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ4858830.1"
                     /translation="MSTIELIALILTSTLVYSTPLILTSLGGTFSERSGIVNVGLEGI
                     MVMGAFSSIVFNLTMSPSLGSMTPWIAILVGGVVGILFSLIHAVATVSLRADHIISGT
                     VINLMAPALGVFLIKAWYGKGQTDNITQNLGYFSFPGLADIPVIGPIFFKNTFLPAYC
                     AILIAVLAWVIIFKTKFGLRLRSVGENPQAADTLGINVYAMRYSGVLISGLLGGMGGA
                     VFAQSISGNFSAGTIVGQGFISMAAMIFGKWNPLGAMGASLFFGFAQSLSIIGAQLPV
                     ISSIPPVLLQIAPYLLTIIVLVVFLGKASGPKANGKNYIKSK"
     gene            20779..21945
                     /gene="deoB"
                     /locus_tag="IXT20_000354"
     CDS             20779..21945
                     /gene="deoB"
                     /locus_tag="IXT20_000354"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HAQ4858831.1"
                     /translation="MFKRVHLIVMDSVGIGEAPDADKFGDVGSDTLGHIAKEAGLNVP
                     HLEQLGLGTIRPLEGVESVKDHQGYATKLEEVSVGKDTMTGHWEIMGLNIKTPFRVFP
                     NGFPDDLIKQIEEFSGRKVVCNKPYSGTAVIDDYGEHQMKTGDLIVYTSADPVLQIAA
                     HEEIIPLEELYRICQFVRDITKDDPYMIGRIIARPYVGEPGDFTRTSNRHDYALNPFG
                     HTVLDSLKEAGKDVIAVGKINDIFNGQGITESVRTKSNMDGVDQLLKVMKNDFSGISF
                     TNLVDFDALYGHRRDVVGYAHAIEEFDLRIPELLEAMEEDDLLMITADHGNDPTFTGT
                     DHTREYVPLLVYSKKMKEQGALPQGYYSDISATIAENFSVSSTENGESFLKLLK"
     gene            21969..22787
                     /locus_tag="IXT20_000355"
     CDS             21969..22787
                     /locus_tag="IXT20_000355"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HAQ4858832.1"
                     /translation="MTKLSEMLQETTQFILDKGVKEIDFGLILGSGLGELADEIEDAV
                     MISYDEIPFFPVSTVVGHAGKLVYGSLAGKKVLAMQGRFHYYEGHSMQTVTYPVRVMA
                     GLGAHSVIVTNACGGVNESFVPGDLMLITDHINFTGENPLIGPNEDAMGPRFPDMSQA
                     YTPDYQATAKAAAEKLGLDLKEGVYMGYSGPTYETPAEIRMSRTIGADAVGMSTVPEV
                     IVAAHSGLKVLGISCITNLAAGMQANLSHEEVVETTQRVKQSFKALVKETLTLL"
     gene            22804..23511
                     /gene="deoD"
                     /locus_tag="IXT20_000356"
     CDS             22804..23511
                     /gene="deoD"
                     /locus_tag="IXT20_000356"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HAQ4858833.1"
                     /translation="MSVHIEAKEGEIADKILLPGDPLRAKYIAETFLEDPVCYNQVRG
                     MLGFTGKYKGERISVQGTGMGMPSATIYAHELIQSYGVKKLIRVGTCGALSKDVHVRD
                     LVLAQGAATSSSMIEKNFQAFHFPPISDFNLLLKAYEIAKEKGYTVHVGNVLSEDSFY
                     KDDLTETFQLAELGVLGVEMEAAALYYLGAKYHVQTLSLMTVSDHLITGEETTAAERQ
                     STFNEMIEVGLETAIAE"
     gene            complement(23809..24495)
                     /gene="gpmA"
                     /locus_tag="IXT20_000357"
     CDS             complement(23809..24495)
                     /gene="gpmA"
                     /locus_tag="IXT20_000357"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HAQ4858834.1"
                     /translation="MPKLVFSRHGLSEWNALNQFTGWADVNLAPEGIEEAKEGGRKIK
                     EAGIEFDVAYTSVLTRAIKTCNLILEYSDQLWVPQIKSWRLNERHYGKLQGLNKKETA
                     EKYGDEQVHIWRRSYDTLPPLMEETDEGSAANDRRYAMLDKRDIPGGENLKVTLERAL
                     PFWQDEIAPALLDNKTVLVAAHGNSLRALAKHIEGISDEDIMDLEIPTGQPLVYELND
                     DLTVAKKYYL"
     gene            complement(24667..25248)
                     /locus_tag="IXT20_000358"
     CDS             complement(24667..25248)
                     /locus_tag="IXT20_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAQ4858835.1"
                     /translation="MKIIGYARTTINDVDLTTQVDQLSDFGCQEIFHETYEVEQNDST
                     AVHLESVIADMQQGDALVICQLHRLGKSTRQLTELTQLFKDRGLHLISLDEGIDTREM
                     GKIYFQLMENLANMECALIKERTLVGLDEARKKGKIGGRPKIDAKTIKKIRRLYYEKK
                     ETIQFISSKCGVSVGTCYKYINLPEKEIEQLCI"
     gene            complement(25381..26064)
                     /gene="rpiA"
                     /locus_tag="IXT20_000359"
     CDS             complement(25381..26064)
                     /gene="rpiA"
                     /locus_tag="IXT20_000359"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HAQ4858836.1"
                     /translation="MNLKQLAGIEAAKFVKDGMIVGLGTGSTAYYMVEEIGRRMREEG
                     LRITGVTTSNATKEQAEKLGIPLKSIDEVPVVDLTIDGADEISADFQGIKGGGAALLF
                     EKIVATYSKETIWIVDSSKLVDKLGKFPLPVEVIPYGSQQLLHIFEEKRFQPVLRTDE
                     NGEVLTTDGGHYIIDLHLEVIEQPESLATYLDELVGVVEHGLFLNMVSKVVVGSEKGV
                     EILDAIRRK"
     gene            26383..26796
                     /gene="rpsL"
                     /locus_tag="IXT20_000360"
     CDS             26383..26796
                     /gene="rpsL"
                     /locus_tag="IXT20_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007085295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HAQ4858837.1"
                     /translation="MPTINQLVRKPRKSKVEKSNSPALNKGYNSFKKTQTNVNSPQKR
                     GVATRVGTMTPKKPNSALRKYARVRLSNLIEVTAYIPGIGHNLQEHSVVLLRGGRVKD
                     LPGVRYHIVRGALDTAGVNDRKQSRSKYGTKKPKA"
     gene            26880..27350
                     /gene="rpsG"
                     /locus_tag="IXT20_000361"
     CDS             26880..27350
                     /gene="rpsG"
                     /locus_tag="IXT20_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HAQ4858838.1"
                     /translation="MPRKGPVAKRDVLPDPIYNSKLVTRLINRVMVDGKRGIAANIIY
                     NAFDLIKESTGNDPLEVFEQAMKNVQPVLEVKARRVGGSNYQVPVEVRPERRVTLGLR
                     WVVNYARLRGEHTMEERLAKEIMDAANNTGASVKKREDTHKMADANRAFAHYRW"
     gene            27444..29531
                     /gene="fusA"
                     /locus_tag="IXT20_000362"
     CDS             27444..29531
                     /gene="fusA"
                     /locus_tag="IXT20_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HAQ4858839.1"
                     /translation="MTREFSLQNTRNIGIMAHIDAGKTTATERILYYTGKIHKIGETH
                     EGASQMDWMEQEQERGITITSAATTAQWKGHRINIIDTPGHVDFTVEVERSLRVLDGA
                     VTVLDAQSGVEPQTETVWRQATTYGVPRIVFANKMDKIGADFLYSVSTLHDRLQANAH
                     PIQLPIGAEDDFTGIIDLVTMKAEMYTNDLGTEIEETEIPEEYRELAEEWREKLVEAV
                     AETDEELTLKYLEGEEITEAELKEGIRRATVNVEFYPVLCGSAFKNKGVQLLLDAVLD
                     YLPSPLDIPAIKGIDPKTDEEVERPADDSAPFSALAFKVMTDPFVGRLTFFRVYSGVL
                     QSGSYVQNATKGKRERVGRILQMHANSRSEISEVYAGDIAAAVGLKDTTTGDTLCDEK
                     SLVILESMEFPEPVIQVAIEPKSKADQDKMGVALQKLSEEDPTFRAETNVETGETIIA
                     GMGELHLDIIVDRMRREFKVEANVGAPQVSYRETFRAGTQAEGKFVRQSGGKGQYGHV
                     WIEFTPNEEGAGFEFENAIVGGVVPREYIPAVETGLKDAMENGVLAGYPLVDIKAKLY
                     DGSYHDVDSNETAFRVAASMALRAAAKKANPVILEPIMAVEVVIPEDYLGDVMGHVTA
                     RRGRVEGMEARAGGQQVVRAMVPLAEMFGYATTLRSATQGRGTFTMTFDHYEDVPKSV
                     QEEIIKKNGGKAE"
     gene            29703..30890
                     /gene="tuf"
                     /locus_tag="IXT20_000363"
     CDS             29703..30890
                     /gene="tuf"
                     /locus_tag="IXT20_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HAQ4858840.1"
                     /translation="MAKEKFDRSKPHVNIGTIGHVDHGKTTLTAAITTVLSKKNGGQA
                     MAYDQIDGAPEERERGITISTAHVEYETDTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVSAADGPMPQTREHILLSRQVGVPYIVVFLNKVDMVDDEELLELVEMEVRDLLTE
                     YEFPGDDVPVVAGSALKALEGDASYEEKILELMAAVDEYIPTPERDNDKPFMMPVEDV
                     FSITGRGTVATGRVERGQVRVGDEVEVVGIAEETSKTTVTGVEMFRKLLDYAEAGDNI
                     GALLRGVAREDIQRGQVLAKPGTITPHTKFSAEVYVLTKEEGGRHTPFFTNYRPQFYF
                     RTTDVTGVVELPEGTEMVMPGDNVTMEVELIHPIAIENGTKFSIREGGRTVGAGVVTE
                     IKA"
     gene            31297..31605
                     /gene="rpsJ"
                     /locus_tag="IXT20_000364"
     CDS             31297..31605
                     /gene="rpsJ"
                     /locus_tag="IXT20_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HAQ4858841.1"
                     /translation="MAKQKIRIRLKAYEHRILDQSADKIVETAKRTGASVSGPIPLPT
                     ERSLYTIIRATHKYKDSREQFEMRTHKRLIDIVNPTPKTVDALMKLDLPSGVNIEIKL
                     "
     gene            31636..32265
                     /gene="rplC"
                     /locus_tag="IXT20_000365"
     CDS             31636..32265
                     /gene="rplC"
                     /locus_tag="IXT20_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HAQ4858842.1"
                     /translation="MTKGILGKKVGMTQIFTESGELIPVTVVEATPNVVLQVKTMETD
                     GYEAIQVGYQDMREVLSNKPAKGHVAKANTAPKRFIREFKNVELGEYEVGKEITVDVF
                     QAGDIIDVTGTTKGKGFQGVIKRHGQSRGPMAHGSRYHRRPGSMGPVAPNRVFKNKRL
                     AGRMGGNRVTIQNLEVVRVDAERNVILIKGNVPGAKKSLITIKSAVKAK"
     gene            32293..32916
                     /gene="rplD"
                     /locus_tag="IXT20_000366"
     CDS             32293..32916
                     /gene="rplD"
                     /locus_tag="IXT20_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HAQ4858843.1"
                     /translation="MPNVALFKQDGSQNGEITLNEEIFGIEPNESVVYDAIVMQRASL
                     RQGTHAVKNRSAVRGGGRKPWRQKGTGRARQGSIRSPQWRGGGVVFGPTPRSYSYKLP
                     KKVRRLAMKSVLSEKVAENNLVAIEGLNFDAPKTKEFKQVLANLSIDSKVLVVLEKGN
                     DFAALSARNLPNVSVVTSDNVSVLDVVSNTKVLATQTALTQIEEVLA"
     gene            32916..33206
                     /gene="rplW"
                     /locus_tag="IXT20_000367"
     CDS             32916..33206
                     /gene="rplW"
                     /locus_tag="IXT20_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HAQ4858844.1"
                     /translation="MNLLDVIKRPVITEKSMLAMDDKKYTFEVDTRANKTLVKQAVEA
                     AFDVKVKNVNIVNVRAKFKRMGKYAGYTKKRRKAIVTLTEDSKEIQLFEAAE"
     gene            33245..34078
                     /gene="rplB"
                     /locus_tag="IXT20_000368"
     CDS             33245..34078
                     /gene="rplB"
                     /locus_tag="IXT20_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010747214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HAQ4858845.1"
                     /translation="MAIKKYKPTSNGRRNMTGSDFAEITTSTPEKSLLQPLKNHAGRN
                     NNGRITVRHQGGGHKRQYRVIDFKRNKDNVVATVQTIEYDPNRSANIALVHYEDGVKA
                     YILAPKGLEVGMKLVSGPEADIKVGNALPLENIPVGTVIHNIEMKPGKGGQLIRSAGT
                     SAQVLGKEGKYVLIRLNSGEVRMILAACRATIGSVGNEQHELINIGKAGRSRWMRKRP
                     TVRGSVMNPNDHPHGGGEGKAPIGRKAPVSPWGQPALGYKTRNKKAKSDKLIVRRRKN
                     K"
     gene            34121..34399
                     /gene="rpsS"
                     /locus_tag="IXT20_000369"
     CDS             34121..34399
                     /gene="rpsS"
                     /locus_tag="IXT20_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HAQ4858846.1"
                     /translation="MGRSLKKGPFVDEHLMKKVEAQAGVEKKKVIKTWSRRSTVFPSF
                     VGYTIAVYDGRKHVPVYIQEDMVGHKLGEFAPTRTYRGHAADDKKTKR"
     gene            34422..34769
                     /gene="rplV"
                     /locus_tag="IXT20_000370"
     CDS             34422..34769
                     /gene="rplV"
                     /locus_tag="IXT20_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HAQ4858847.1"
                     /translation="MAEQITSAKATAKTVRTSPRKARLVIDLIRGKSVADAISILKFT
                     PNKSAGIIEKVLMSAVANAENNFDLDVENLVVSEAFVNEGPTMKRFRPRAKGSASPIN
                     KRTSHITVVVSEK"
     gene            34783..35439
                     /gene="rpsC"
                     /locus_tag="IXT20_000371"
     CDS             34783..35439
                     /gene="rpsC"
                     /locus_tag="IXT20_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HAQ4858848.1"
                     /translation="MGQKVHPIGMRVGIIRDWDAKWYAEKEYAEFLHEDLRIRKFIST
                     KLADAAVSTVEIERATGRVNISIHTAKPGMVIGKGGSEVESLRKELNKLTGKRIHINI
                     VEIKKPDLDAKLVGEGIARQLENRVAFRRAQKQAIQRSMRAGAQGIKTQVSGRLNGAD
                     IARSEGYSEGTVPLHTLRADIDYAWEEADTTYGKLGVKVWIYRGEILPTKKNTEKGGK
                     "
     gene            35442..35876
                     /gene="rplP"
                     /locus_tag="IXT20_000372"
     CDS             35442..35876
                     /gene="rplP"
                     /locus_tag="IXT20_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HAQ4858849.1"
                     /translation="MLVPKRVKHRREFRGKMRGEAKGGKEVAFGEYGLQAVDSHWITN
                     RQIEAARIAMTRYMKRGGKVWIKIFPHKSYTAKAIGVRMGSGKGAPEGWVAPVKRGKI
                     MFEIAGVPEEVAREALRLASHKLPMKTKIVKREEMGGESNEG"
     gene            35866..36054
                     /gene="rpmC"
                     /locus_tag="IXT20_000373"
     CDS             35866..36054
                     /gene="rpmC"
                     /locus_tag="IXT20_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HAQ4858850.1"
                     /translation="MKVKEIRELTTAEMLDQEKQLKEELFNLRFQLATGQLENTARIK
                     EVRKSIARIKTVLREQAK"
     gene            36079..36345
                     /gene="rpsQ"
                     /locus_tag="IXT20_000374"
     CDS             36079..36345
                     /gene="rpsQ"
                     /locus_tag="IXT20_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HAQ4858851.1"
                     /translation="MTEGRNQRKVYQGRVVSDKMDKTITVAVETKKNHPIYGKRMNYS
                     KKYKVHDENNTAKVGDIVRIMETRPLSATKRFRLLEVVEEAVII"
     gene            36403..36771
                     /gene="rplN"
                     /locus_tag="IXT20_000375"
     CDS             36403..36771
                     /gene="rplN"
                     /locus_tag="IXT20_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002460061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HAQ4858852.1"
                     /translation="MIQAESRLKVADNSGAREILTIKVLGGSGRKTANIGDVIVATVK
                     QATPGGVVKKGDVVKAVIVRTKSGARRTDGSYIKFDENAAVIIRDDKSPRGTRIFGPV
                     ARELRENNFMKIVSLAPEVL"
     gene            36808..37116
                     /gene="rplX"
                     /locus_tag="IXT20_000376"
     CDS             36808..37116
                     /gene="rplX"
                     /locus_tag="IXT20_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HAQ4858853.1"
                     /translation="MFVKKGDKVKVITGKDKNKEGVVLAAFPKKDKVIVEGVNVVKKH
                     QKPSQAAPQGGIVEMEAPIHVSNVMVVDSTGVAGRVGYKEVDGKKVRVSKKTGEVLDK
                     "
     gene            37144..37683
                     /gene="rplE"
                     /locus_tag="IXT20_000377"
     CDS             37144..37683
                     /gene="rplE"
                     /locus_tag="IXT20_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016185989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HAQ4858854.1"
                     /translation="MNRLKEKYLKEVTPSLMEKFNYSSVMQTPKVEKIVINMGVGDAV
                     SNAKNLDKAVEELTLITGQKPMITKAKKSIAGFRLREGMPIGAKVTLRGERMYEFLDK
                     LVSVSLPRVRDFHGVSKKAFDGRGNYTLGIKEQLIFPEVDYDLVDKVRGMDIVIVTTA
                     NSDEESRELLTQLGMPFQK"
     gene            37702..37887
                     /locus_tag="IXT20_000378"
     CDS             37702..37887
                     /locus_tag="IXT20_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011245027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HAQ4858855.1"
                     /translation="MAKKSMIAKNKRPAKFSTQAYTRCERCGRPHSVYRKFHLCRICF
                     RELAYKGQIPGVKKASW"
     gene            37923..38321
                     /gene="rpsH"
                     /locus_tag="IXT20_000379"
     CDS             37923..38321
                     /gene="rpsH"
                     /locus_tag="IXT20_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010742763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HAQ4858856.1"
                     /translation="MVMTDPIADFLTRIRNANMVKHESLEVPASKIKRDIAEILKREG
                     FVRDVEYIEDDKQGVIRVFLKFGKNGERVITNLKRISKPGLRAYVKADEVPKVLNGLG
                     IAIISTSEGVVTDKEAREKNIGGEVIAYVW"
     gene            38353..38889
                     /gene="rplF"
                     /locus_tag="IXT20_000380"
     CDS             38353..38889
                     /gene="rplF"
                     /locus_tag="IXT20_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HAQ4858857.1"
                     /translation="MSRIGNKVVVIPEGVTVTQDGNNVTVKGPKGELTRTFSADIKMN
                     IEGNEVTFTRPNDSKEMKTIHGTTRANFNNMVVGVSEGFQKALELIGVGYRAQLQGSK
                     LVLNVGYSHPVEITPPAGVTVEVPANTQVIVKGANKEEVGELAANIRGTRPPEPYKGK
                     GIRYVGEYVRRKEGKTGK"
     gene            39050..39406
                     /gene="rplR"
                     /locus_tag="IXT20_000381"
     CDS             39050..39406
                     /gene="rplR"
                     /locus_tag="IXT20_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014125626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HAQ4858858.1"
                     /translation="MITKPDKNKTRQKRHRRVRNNISGTAERPRLNVFRSNKNIYAQI
                     IDDVAGVTLASASTLDKEISGGTKTEAAQAVGKLVAERAAEKGIKVVVFDRGGYLYHG
                     RVQALAEAARENGLEF"
     gene            39427..39927
                     /gene="rpsE"
                     /locus_tag="IXT20_000382"
     CDS             39427..39927
                     /gene="rpsE"
                     /locus_tag="IXT20_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HAQ4858859.1"
                     /translation="MVYIDPKHLELEDRVVAINRVTKVVKGGRRLRFAALVVVGDKNG
                     HVGFGTGKAQEVPEAIRKAIEDAKKNLVEVPMVGSTIPHEVIGAFSGGRILMKPAVEG
                     SGVAAGGPVRAVLELAGVADITSKSLGSNTPINVVRATVEGLKQLKRAEEVAELRGKS
                     VEELIG"
     gene            39942..40121
                     /gene="rpmD"
                     /locus_tag="IXT20_000383"
     CDS             39942..40121
                     /gene="rpmD"
                     /locus_tag="IXT20_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HAQ4858860.1"
                     /translation="MAELKVTLKRSIIGRPQNQRDTVKALGLTKINSSVVKPSNEAIK
                     GMINTVSHLVDVEEI"
     gene            40165..40605
                     /gene="rplO"
                     /locus_tag="IXT20_000384"
     CDS             40165..40605
                     /gene="rplO"
                     /locus_tag="IXT20_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HAQ4858861.1"
                     /translation="MKLHELKPAEGSRQVRNRVGRGTSSGNGKTAGRGQKGQKARSGG
                     GVRLGFEGGQTPLFRRLPKRGFTNVNRKDYAVINLDVLNRFEDGAEVTPVALVEAGIV
                     KNEKAGIKVLANGELNKKLTVKAAKFSKAAQEAIEAAGGSIEVI"
     gene            40605..41900
                     /gene="secY"
                     /locus_tag="IXT20_000385"
     CDS             40605..41900
                     /gene="secY"
                     /locus_tag="IXT20_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HAQ4858862.1"
                     /translation="MFRLLKDAFKVKDIRSKILFTVFVLFVFRLGAHITVPGVNAASL
                     QNLSSLPFFNMLNLVSGSAMQNFSIFSMGVSPYITASIVIQLLQMDIVPRFVEWSKQG
                     EVGRKKLNQATRYLTLVLAFIQSVGITAGFNQYAQLGFVNNPNVATYVTIGLILTAGT
                     MFVTWLGEQITEKGIGNGVSMIIFAGIISRLPESIREIYEDYFINIDPSDIWKSAIFV
                     AILLVAVLLIVTFVTFFQQAERKIPIQYTKRVAGAPTSSYLPLKVNAAGVIPVIFASS
                     FIATPNAVLQALSGRFGSENWYKVMMEIFNYNTAPGATIYTILIVAFTFFYAFVQVNP
                     EKLAENLQKQGSYIPSVRPGKGTEEYVSRLLMRLSTVGAVFLGLVALLPIIAQMVWDL
                     PQSIGLGGTSLLIVIGVALETAKQLEGLMLKRKYVGFIE"
     gene            41962..42609
                     /locus_tag="IXT20_000386"
     CDS             41962..42609
                     /locus_tag="IXT20_000386"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HAQ4858863.1"
                     /translation="MNLILMGLPGAGKGTQAEKIIDAYGIPHISTGDMFRAAMQNETA
                     LGLEAKSYMDKGALVPDEVTNGIVKERLAEPDTEKGFLLDGFPRTLDQAKALDAMLKD
                     LNKKIDAVIDIHVGEEILVERLAGRFICSNCGATYHKIFNPTKVEDTCDRCGGHEFYQ
                     REDDKPETVKNRLAINIKNSEPILAYYKEQGLLNTIDGAREIDAVFADVQKIIEK"
     gene            42800..43018
                     /gene="infA"
                     /locus_tag="IXT20_000387"
     CDS             42800..43018
                     /gene="infA"
                     /locus_tag="IXT20_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HAQ4858864.1"
                     /translation="MAKEDMIEVEGTVVETLPNAMFKVELENGHQVLATVSGKIRMHY
                     IRILPGDKVTVELSPYDLNRGRITYRFK"
     gene            43051..43167
                     /gene="rpmJ"
                     /locus_tag="IXT20_000388"
     CDS             43051..43167
                     /gene="rpmJ"
                     /locus_tag="IXT20_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HAQ4858865.1"
                     /translation="MKVRPSVKPMCEHCKVIRRKGRVMVICPANPKHKQRQG"
     gene            43185..43550
                     /gene="rpsM"
                     /locus_tag="IXT20_000389"
     CDS             43185..43550
                     /gene="rpsM"
                     /locus_tag="IXT20_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HAQ4858866.1"
                     /translation="MARIAGVDIPRDKRVVVSLTYIYGIGNTTAKKILADAGVSEDVR
                     VRELTNEQTDAIRAEVDKLKVEGDLRREVNLNIKRLMEIGSYRGIRHRRGLPVRGQNT
                     KNNARTRKGPARAIAGKKK"
     gene            43578..43967
                     /gene="rpsK"
                     /locus_tag="IXT20_000390"
     CDS             43578..43967
                     /gene="rpsK"
                     /locus_tag="IXT20_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HAQ4858867.1"
                     /translation="MAAKKVNRKRRVKKNIEAGIAHIHSTFNNTIIMITDTHGNALAW
                     SSAGSLGFKGSKKSTPFAAQMAAETATKAAMEHGLKTVEVTVKGPGSGREAAIRSLQA
                     TGLEVTAIRDVTPVPHNGARPPKRRRV"
     gene            44047..44985
                     /locus_tag="IXT20_000391"
     CDS             44047..44985
                     /locus_tag="IXT20_000391"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010774253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HAQ4858868.1"
                     /translation="MIEFEKPRIAKIDEEKDYGKFIVEPLERGYGTTLGNSLRRILLS
                     SLPGAAITNIQIDGVLHEFSTIKGVREDVTQIILNIKGLALKMYGQEEKTLEIDITGP
                     ATVTAGDIIVDSDVEILNKDMYICSVSEGATFHARLTVKPGRGYVQADENKKEDMPIG
                     VLPVDSIYTPVRRVNYQVENTRVGHREDFDKLTMEIWTDGSIEPLEAMSLSAKIMTEH
                     LDIFVNLTDEAKNAEIMVEKEETQKEKMLEMTIEELDLSVRSYNCLKRAGINTVQELT
                     NKSEPEMIKVRNLGRKSLEEVKAKLHDLGLGLRKED"
     gene            45013..45393
                     /gene="rplQ"
                     /locus_tag="IXT20_000392"
     CDS             45013..45393
                     /gene="rplQ"
                     /locus_tag="IXT20_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HAQ4858869.1"
                     /translation="MSYRKLGRTSSQRKAMLRDLTTDLLINERIVTTEARAKEVRSTA
                     EKMITLGKRGDLHARRQAAAFVRNEVASVREENEDIVIESALQKLFNDIAPRYAERQG
                     GYTRILKTEPRRGDGAPMVVLELV"
     gene            45665..46504
                     /locus_tag="IXT20_000393"
     CDS             45665..46504
                     /locus_tag="IXT20_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HAQ4858870.1"
                     /translation="MEPIIELEKINYKYQPDDLRPALKDVSFTIDKGEWIAIIGHNGS
                     GKSTLAKTINGLLLPESGIVKVGNQILDEENIWTIRQMVGMIFQNPDNQFVGSTVEDD
                     VAFGLENQGIPREEMLVRVKDALEKVRMAEFASREPARLSGGQKQRVAIAGVVALRPD
                     IIILDEATSMLDPEGREEVISTIKKIKEESQLTVISITHDIDEAANANRILVMRQGEL
                     VREGTPKEIFSAGPELIDLGLDLPFPEKLKSALKERGVDVPSEYMTEERMVDWLWTSV
                     LNK"
     gene            46480..47349
                     /locus_tag="IXT20_000394"
     CDS             46480..47349
                     /locus_tag="IXT20_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HAQ4858871.1"
                     /translation="MDIRFEQVDFTYQPNTPFEQRALFDINMTIKENSYTALVGHTGS
                     GKSTLLQHLNALVKPTSGTVHIGERDIQPDTDNKNLKPIRKKVGIVFQFPEAQLFEET
                     VAKDIAFGPKNFGVSEEEALVLAKETLEQVGLDESYLERSPFELSGGQMRRVAIAGVL
                     AMRPEVLVLDEPTAGLDPQGRKEMMEMFWRLHKEQQITIVLVTHLMDDVANFADYVYV
                     LEKGRIVNSGEPQEVFQNIEWLKEKQLGVPTATEFAEELMAKGMNFATLPLTAEELAD
                     AIVHYAGGNAHDE"
     gene            47339..48136
                     /locus_tag="IXT20_000395"
     CDS             47339..48136
                     /locus_tag="IXT20_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HAQ4858872.1"
                     /translation="MMNKLIFGRYMPGDSLIHRLDPRAKLLASFYFIGIIFLANNWET
                     YLLLAFFTLLSVFLSKVDLKFFIRGIRPLIWLILFTVALQVLFTSGGETYWSWGVFHI
                     TEFGVMNGLFIFCRFVLIIFMSTLLTLTTPPLELSDAIEYLLRPLKAVRFPVHEISLM
                     LSIALRFVPTLMDETEKIMNAQRARGVDFGEGNLVQKMKAVIPLLIPLFVSSFNRAED
                     LATAMEARGYKGGEGRTKYRVLHWHNRDTMVIIAFVLLTVLLIFLRG"
     gene            48339..49085
                     /gene="truA"
                     /locus_tag="IXT20_000396"
     CDS             48339..49085
                     /gene="truA"
                     /locus_tag="IXT20_000396"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HAQ4858873.1"
                     /translation="MVRYKAIIAYDGTNFNGFQKQPNGRTVQEEVEKTLQKMANGKEI
                     TVFGSGRTDAGVHAIGQVIHFDYPEERPLERMRFALDTQSPEDIAVRQVEIVSEDFHA
                     RYLVKEKTYQFRVDIGKPRSPFRRHYASYFPYPLDLEKIQRALPDLLGTHDFTSFCAS
                     GSSIEDKIRTIYEAKMEVNETKDELLFTFRGNGFLYKMIRILVGTLLKIGNGRLPEDS
                     IPDIIAKKDRNAAGPTAHPEGLYLYEVKYE"
     gene            complement(49418..52087)
                     /locus_tag="IXT20_000397"
     CDS             complement(49418..52087)
                     /locus_tag="IXT20_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HAQ4858874.1"
                     /translation="MKKNLKSFFRENGRYAAASFLIPFLILALVYLSIGIYPGSSRSI
                     LASDAFSQFSNFHASFRNVLLGKQSIFYTWNASLGLNYLSLISYYLGGIFTPLVFFFP
                     NQMMPDALYVLTLLKVGCAGLAFWFLARTFKIPRWGHVALSVSYALISFITAHSEIIM
                     WLDAFVYLPLVILGIHRVMDLKRPKLLFISYLLLFLSSFYMGFMIGVFSFLYFIARLL
                     TNWSEYKKTIVPYGITSLLAGGASMIIVLPAVLDLRSNGEALSQITTLKTEATSYLDL
                     IMKNMIGVYDTTKYGSIPFVYAGLLPLVLCLFYFASRKVPLKNKFLYGSLFAILIASF
                     YLVPLNLFWHGMHAPNMFLFRYAYLFSFLVLLLAGKGWEKIEQDDGGLLLGIILVVAA
                     LFAVAWGIKPEGSYTYVTMTSFVLTVVFLGIYAFAVGFYQHKKLSFNHLSILLLLIMS
                     AEALVNTNSMIHGILDDWNYASRSLYTDPYPDLKALVNETKKDSDSFYRLENLDPVSA
                     NDSINYGYSGISLFSSIRNRNSSSYLDKLGFRSRGTNLNIRYNNNTLLMDGFTGIKYN
                     ISKNESTFSKYGFRQAGQSGDYTLYQNVNALPLAFTAPLSVNDVEQPVTDNLTSQTNL
                     INTLSGLNEQYFTFYTPTLKNQLNTTITKTTTGVTYSETADNQPKELTWEVHVPANTQ
                     AYLSLFPTNFAQLESSSATITVNGTSRKTQINISGQYYDLGYYPQETTVTFTASFYGT
                     KEISFMEPKVVGLDVVAYQAAMNQIKEKGVEMEATGRKASGTVNTAEDSMLVTTIPYD
                     KGWKAKIDGKPVKVSNFKDAFLMIKVPAGKHTVTFSYLPEGFTIGAILFVLCIALFIL
                     YVWYLDRNRPFYGTIDPAIEEQPRNTHRRRRRK"
     gene            complement(52325..52513)
                     /locus_tag="IXT20_000398"
     CDS             complement(52325..52513)
                     /locus_tag="IXT20_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HAQ4858875.1"
                     /translation="MAKVCYFTGRKTKSGNNRSHAMNSTKRTVKPNLQKVRVMVDGKP
                     KKVWVSTRALKSGKVERV"
     gene            52783..53145
                     /locus_tag="IXT20_000399"
     CDS             52783..53145
                     /locus_tag="IXT20_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HAQ4858876.1"
                     /translation="MAVKIQTSAGTIEITNDVIATVVGGAATDVFGVVGMASKNQIKD
                     NITEILRKENYSRGVVVRQEDNGIAVDVYTIISYGTKISEVSRNVQEKVKYNLEALLG
                     VTANSVNVFVQGVRVLPD"
     gene            53197..54879
                     /locus_tag="IXT20_000400"
     CDS             53197..54879
                     /locus_tag="IXT20_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HAQ4858877.1"
                     /translation="MSVTEISASQFQEMVQAGANRLQVNAEYVNSLNVFPVPDGDTGT
                     NMNLSMTSGAKAVVDSTSEKVGELAAMLSKGLLMGARGNSGVILSQLFRGFSKQIPDV
                     VSLNASDLAAAFTHGVETAYKAVMKPVEGTILTVAREAAKAGEKKAKSTDDVVEVMTA
                     VVKGAKRALAKTPDLLPVLKEVGVVDSGGQGLLFVYEGFLSALNGEFQADETYEPSPA
                     EMDDMVNAEHHRSVQGQMATEDIHFGYCTEIMVKIGEGPTVDSTFDYEEFRNYLDGIG
                     DSLLVVNDDEIIKVHVHTENPGEVLNYGQKFGSLVKIKVDNMRLQHETILEHDEEVNE
                     FAKKEIPRIPYGIVAIAAGKGVQELFESLGANYVISGGQTMNPSTEDIVKAIQEVNAD
                     KVIVLPNNKNIFMAADQAAEVADIPVAVVPSRTISQGLTAMLSFNDQASLEENKETMT
                     EMLSSVVSGQVTHAIRDTSIDGVEIKEGDFLGMIDGKILISNPDITATALSTLEQMIN
                     EDTEIVTILAGDEGTVEQANEFAEKLTEKHPDLEIEIHQGDQPVYPYLFSAE"
     gene            54996..57032
                     /gene="recG"
                     /locus_tag="IXT20_000401"
     CDS             54996..57032
                     /gene="recG"
                     /locus_tag="IXT20_000401"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HAQ4858878.1"
                     /translation="MRSLDDPITMLAGVGEKRAASLASLGIQTIEDLLTYYPFRYEDI
                     QERNLNEIQDQEKVTLKGIVVSPPVMNRFGYKKSRLQFRMMQEHDVFNVSFFNQPYLK
                     EKVILSEEIAVYGKWDAKRKALNGMKILGSQTMDDFSPIYHVNKSIRQTTLVDLIKKG
                     FQEYGTLIEENLPNNLVEKYRLLDRASAVRSMHFPKDHEENHQAKRRVVFEEFFLFQM
                     RLQGLKKAEKAETNGLEILYDVQKLKQFTQKLPFELTNAQKRVTNEICRDLRSPQHMQ
                     RLLQGDVGSGKTVVAAIALYATVTAGFQGALMVPTEILAQQHMESLQQLFDPNEVTIA
                     LITGSTKAKERRELLEKLEQGEINVVIGTHALIQDGVEFQNLGLVITDEQHRFGVNQR
                     KVLREKGWRPDVLFMTATPIPRTLAITAYGEMDVSIIDELPAGRIPIETRWVRTPQLD
                     SVLDWTKKELARGHQMYVICPLIEESEALDVKNAVEIYEKLRELFAPKYEVGLLHGKM
                     KNQEKEVIMEAFKDNQMQILVSTTVIEVGVNVPNATVMLIMDADRFGLAQLHQLRGRV
                     GRGSDASYCILVANPKNELGVERMKIMTETTNGFVLSEKDLELRGPGEVFGFRQSGLP
                     QFAIADLVTDGNVLEVAREEAAALWQIKDWQMLPEYQPLAQSLQIENQDNRYFD"
     gene            57081..58082
                     /gene="plsX"
                     /locus_tag="IXT20_000402"
     CDS             57081..58082
                     /gene="plsX"
                     /locus_tag="IXT20_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HAQ4858879.1"
                     /translation="MKIAVDAMGGDHAPQAIVEGVALAQQDFPEVEFLLYGKEAEIKK
                     YLTNEKNITIIHTDEKINSDDEPVKAIRRKKTASMVLAAQAVKNGEADAVFSAGNTGA
                     LLAAGLFIVGRIKNIERPGLMSTLPVVGKEGAGFDMLDLGANAENKPEHLLQYGILGS
                     FYARKVRGIGRPRVALLNNGTEATKGSEVTKKAYELLQNETSLNFIGNVEARELLNGV
                     ADVVVTDGFTGNAVLKSIEGTAMNMMNLLKSAILNEGIKGKMGAMLLKDGLRSLKAEM
                     DYSKHGGAVLFGLKAPVIKTHGATGPDAVRYTIRQIHTMLETDVVGQLVEQFEKSEE"
     gene            58204..58446
                     /gene="acpP"
                     /locus_tag="IXT20_000403"
     CDS             58204..58446
                     /gene="acpP"
                     /locus_tag="IXT20_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HAQ4858880.1"
                     /translation="MTREEVFNKVAKIISNHFEIDTDKVTDELNIKDDLKADSISIME
                     FVLELEDEFGTEISDEDAEQIETVGGAVDYISSHLK"
     gene            58714..59718
                     /locus_tag="IXT20_000404"
     CDS             58714..59718
                     /locus_tag="IXT20_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ4858881.1"
                     /translation="MSDNQLLEVQNLHTGFRLKDDYYDAVDDVSFTLDKNEILAIVGE
                     SGCGKSTLATTIMGLLDPNNTKITGEILYNDLNLIDLNETLYNKIRGNDIGMIFQDPL
                     SALNPLMRIEDQIKEGLSYHTKMNAEQRQARALELLEQVGIPNPARVGRQYPHELSGG
                     MRQRVIIAIAIACKPPIVIADEPTTALDVTIQAQILDLLKDLQEETKTGIILITHDLG
                     VVAEMADRVAVMYGGQFVEVAGVKELFDHPKHPYTRSLLNSIPQEGNHDAELHVIEGV
                     VPSLKNMPRKGCRFAPRIPWIPASDHEEEPILHEVAPNHLVRCSCYKHFHFRDEKGEV
                     "
     gene            59719..60663
                     /locus_tag="IXT20_000405"
     CDS             59719..60663
                     /locus_tag="IXT20_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ4858882.1"
                     /translation="MTELITIKDLKVHYPIRSGFFNRVTDHVYAVDGVDFIIEKGKTY
                     GLVGESGSGKSTTGKAVVGLEKVTAGQIMYEGKDVTKRSNRKKMGYNKDVQMIFQDSM
                     SSLNPKKRVLDIIAEPIRNFERLSDQEEKKKVKGLLDIVGMPEDALYKYPHEFSGGQR
                     QRLGVARAVATNPKLIVADEPVSALDLSVQAQVLNFMKRIQQEYGLSYLFISHDLGVV
                     KHMCDNIAIMYKGRFVEIGTREDIYNDPRHIYTKRLLSAIPQIDVENRESHKENRRRV
                     EQEYIQNQKEYYDATGRVYDLRTITTTHKVALKDGGAS"
     gene            60663..61625
                     /locus_tag="IXT20_000406"
     CDS             60663..61625
                     /locus_tag="IXT20_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ4858883.1"
                     /translation="MWKTILRRVLLMIPQVIILSVLIFLLAQAMPGDPFTGLINPNQD
                     PAVIEQMRQAAGLNDPWYVQYFRWIGNALQGDFGQSFLYKRSVADLIGERIINTLYLS
                     ILTVVLTYLIAVPLGMLAGRYQNSILDKIVVIYNFFSFAVPLFIFGLIMLFVFGYRLG
                     WFPTSGSQTLGLQGGFITQWIDRLKYILLPSITQAFLATAVTIQYLRSEVIDSKSLDF
                     VRTARSKGVPTNKVFSRHIFRNAALPMASQMGYEITSLIAGSVVIEKIFGYPGVGKLF
                     IDSIGQRDYTVITSLVLILGIATLVGTLLSDIIMSIVDPRIRIQ"
     gene            61652..62566
                     /locus_tag="IXT20_000407"
     CDS             61652..62566
                     /locus_tag="IXT20_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ4858884.1"
                     /translation="MSEKNNTAVQESIPPMGIRMIAREFKKDKIAIFSLGLLVVLLLV
                     IFIGAMLTDQDKVMYVSIFDKYAAPGTVSTQGQKFILGADEGGRDVLGQLIIGARNSV
                     TIGFAITILTSIIGVGLGIISGYYGGIIDNILMRIVDFIMILPIMLIIIVFVSIISRY
                     NVWSFIFIMSAFYWVAKARLFRSKTLSEARRDYVSASKTMGTNDFKIMFREIMPNLSS
                     LIITNLTMNFAANIGIETTLTFLGFGLPANVPSLGTLIGYASNGEVLVNRQWIWLPAS
                     ILILVLMLSINYVGQAFKRSADARQRLG"
     gene            62592..64373
                     /locus_tag="IXT20_000408"
     CDS             62592..64373
                     /locus_tag="IXT20_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HAQ4858885.1"
                     /translation="MKKTIFGFAALMSAVVLAACGGGGTDTSGNNSGGNGNNKEELTF
                     PIATKNNDDAIEDGELEAAVATDSQFKGLFQWEFYQDSYDAAFMAPSHEPLFANDDDF
                     RITDDGAATLDLDEDTKKATIKVKDNVKWSDGEDVTADDVIFPYEIIGNKDYTGIRYD
                     DTFRNIVGMEEYHDGSADTISGIKKIDDKTVEIEYKEVNPSMLQSGGGIWSYAAPKHT
                     LEGVAIKDMESSDQVRKNPVTFGPYYMSNIVAGESVEYLPNEHYWNGTPKLKKITMKS
                     VPMASATEALNSKLYDMIFQMPTDTYDTYKEIAGYTNLGREQTSYTYLGFKLGKWNAD
                     TKSVEYNPDSKMADKSLRQAMGYALDNNAVGERFYAGLRSNATSLIPPVFQSYHDDEM
                     KGFTQDLDKAKKLLDDAGYKDVDDDGLREDKDGNKLTINFASMSGGETAQPLADYYIQ
                     QWKEIGLDVQYTTGRLIEFNSFYDKLENDDPEIDVYQAAWNTGTDPNPSGLWGPNAAF
                     NYTRYESEENTKLINDISSSKAFDEEYQKEAYQKWQQYAFEEAFAIPTLFRNEVLPVN
                     DRVKDWDWSYDVPNPWANVSVTADSRS"
     gene            64721..65407
                     /locus_tag="IXT20_000409"
     CDS             64721..65407
                     /locus_tag="IXT20_000409"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HAQ4858886.1"
                     /translation="MDNQLTKELKENYNIVFHDLNLLEQAFTHSSYVNEHRNLQLSDN
                     ERLEFLGDAVLELMVSQYLFRLYPHLPEGKLTKTRAAIVREDSLSKFAKECHFDQYVL
                     LGKGEENSGGRTRPALLCDLFEAFLGALYLDQGFDTTHAFIEKVIFPKVKAGAFSHEM
                     DHKTKLQEVLQKSGDVSIEYRLINEEGPAHERVFWIEVYVDDQLIGTGQGKSKKLAEQ
                     AAAENALAAL"
     gene            65427..69008
                     /gene="smc"
                     /locus_tag="IXT20_000410"
     CDS             65427..69008
                     /gene="smc"
                     /locus_tag="IXT20_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HAQ4858887.1"
                     /translation="MYLKRIEIAGFKSFADKTVIDFENSVTAVVGPNGSGKSNITEAI
                     RWVLGEQSAKSLRGGKMPDIIFAGSDTRKQLNIAEVTVILDNSDHYLPLEYNEISVTR
                     RYRRTGESEFFINKQSCRLKDIQELFLDSGLGKESFSIISQGKVEAIFSSKPEDRRGI
                     FEEAAGVLKYKQRKKKAEQKLFETEDNLSRVQDIIHELEEQLTPLAAQSEAAKEFLRL
                     KETLTQTDVSLMVAEIKTAKKDWDNKQAQLAKFNLELGKLSESIQEQESILAKQRKEN
                     AQADRLIEKNQQVLLDLSEKLKQTEGQKDVLQERTKHTQKSSQEYQTSLAEAQKKVKH
                     FEKLQESLMKAAAEKETEIQKAEANLIKTQQELEKYQKSTKELLAELRDQYVDLMQEQ
                     AAVGNELKYLERQYIQETAKSKQTLAKQSEVEASVDRLILQKEELTQKQAQLKSSLTE
                     TKEKLEMIQQNGKKFQEALAKEQPKMYQLMNQVQQLRARKKSLQEIQENYFGFYQGVR
                     LVLQHKQQLSGIVGAVAELIDVPADFTLAIETALGGAAQHVIVENEKDARQAITYLKQ
                     QRGGRATFLPLTTIKPRQLPAHILTQAAAVEGFIGIASEQVSYPDQIQTVVQNLLGTI
                     LLAKDLTSANAIAQTIRYQYRVVSLEGDVMNAGGSMTGGANKRGNQGSLFVQNQELKQ
                     LTSEFEEADKQLQAQEKKVQELQQETARLAEEQEVLRTRGEQLRFEEQEATNQLQNII
                     NELERFEKEKQISTFETRELQQFIEDYEKQTNELKDKQTDLESQRQKIDEEIKSLSQE
                     SDQMEARRAQVQSQKAQEQADLAVLKEQFNHLQIQLRGARVQKAEATERQEAIEKQLA
                     TLTADFSDHEVTEESLEKQINELSAQRETLKAELAKAKEQRDRRQKEIDKLEAVLAER
                     NREQKARLSEQSKLEVQKDRAEMILDNHLTYLQSEYQLTFEKASQDYQETTDIEDSRT
                     KVSSLKEQIEKLGPVNLNSIEQYEQVSERHTFLATQRDDLLAAKNQLFETMDEMDDEV
                     RTRFKEVFEAIRQEFKIVFPNMFGGGKAELVLTEPTDLLKTGIEIEVQPPGKKLQSLS
                     LLSGGERALTAIALLFSIIRVCPVPFCILDEVEAALDEANVKRFGRYLSDFQDDTQFI
                     VVTHRKGTMVAADVLYGVTMQESGVSKIVSVRMEDINEEAKINETKV"
     gene            69017..69826
                     /locus_tag="IXT20_000411"
     CDS             69017..69826
                     /locus_tag="IXT20_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAQ4858888.1"
                     /translation="MIKLVAIDLDGTLLNEQKLISDENKQALAQAKQQGVKIVLCTGR
                     PLAAMAHYLQELGLVDEGDYSITFNGGLVQKNDTGEIMEKKVMEVSDIQRLYKLAQQL
                     DLPLDVLSDNVVLQLPSAPRKQSLYNVLNKLLQFQPADISDITDEFVLNKAVIAYDQE
                     ELDPKIKEIPAEFHEMYEIIKTRSMLLEFMPKGVTKAYGISLLAKDLGLKQEEIMAIG
                     DEENDLPMIQYAGLGVAMENAVPFVKEAADFVTASNEENGVAKVIQKFVLE"
     gene            69837..70835
                     /gene="ftsY"
                     /locus_tag="IXT20_000412"
     CDS             69837..70835
                     /gene="ftsY"
                     /locus_tag="IXT20_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HAQ4858889.1"
                     /translation="MGFFDKIKRAFTGEEKTEEVEVQEKYDKGLEKTRKTFGERMNEL
                     FANFRTVDEDFFEELEETLIGADVGFETAIRITEALRQEVKLRNAKKSAEVQNAIIEK
                     LVDLYEAEGINEVNELNIQEDGLTVILFVGVNGVGKTTSIGKLAHQFKNEGKKVLMAA
                     ADTFRAGAIDQLVVWGERAGVEVVRGNAGGDPAAVVFDALERAKTEQADVLLVDTAGR
                     LQNKVNLMNELEKIKRVIQRELPEAPQETLLVLDATTGQNAMVQAKQFKETTDVTGLV
                     LTKLDGTAKGGIVLAIRNELHLPVKLVGLGEGIDDLEPFDPNDFVVGLFKGLLKEE"
     gene            71022..73289
                     /gene="gshAB"
                     /locus_tag="IXT20_000413"
     CDS             71022..73289
                     /gene="gshAB"
                     /locus_tag="IXT20_000413"
                     /EC_number="6.3.2.2"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate--cysteine ligase
                     GshA/glutathione synthetase GshB"
                     /protein_id="HAQ4858890.1"
                     /translation="MMNFKQLLLHVNARPFIDQARFGIEREGQRVDLAGNLAKTDHPA
                     IFGDRSYHPYIQTDFSETQTEMITPVTDSIPELFQYLAAVYDVTARSIPKEEMIWPLS
                     MPPALPEKDEEIIIAKLKNFEDVLYRRYLAKEYGKRKQMVSGIHFNFEFGDELLRTLF
                     SHQEEFQDFSEFKTELYLKTARNFMRYRWMITYLFGASPMSEKNYFLDESHPQEPVRS
                     IRNSALGYTNHPNVKVSYASMKQYLADIERMIEEGKLSEEKEFYTPLRFRGGKKVADL
                     ATTGVRYIELRNIDLNPYARLGINPEQVRFLQLFLMYMLWTEEKEDCDQWVAEGTTRN
                     NKVALEQPSDQTEFHQEGREILEGMKQMLVELDWLDSLYLVEEALTQMDHPEQTLAAK
                     LYQEAQLSSQQEVAVALGHQYYKESHERPYQLAGFREMELSTQIFMFDAIQKGVQVKV
                     LDESDQFLRLQFQDHVEYVKNANMTSKDSYIVPLIMENKTVTKKVLKEAGFRVPGGAE
                     FSSMEEAVKAYPRFAEQAFVIKPKSTNYGLGITIFKEGASLEDYQAGLAIAFREDSSV
                     LVEEFMPGTEYRFFVIDGEVQAIMLRVPANVIGDSIRTVKELVEEKNSDPLRGTNHRA
                     PLELIQLGELEQLMLKEQGLTIESVPQANQIVYLRENSNISTGGDSIDMTDEFSEAYK
                     KIAVSAVEALGAKISGIDLIIPDKEIDPTTDKKAYGIIEANFNPAMHMHVYPFAGKGR
                     RLTMNVLKLLYPEVF"
     gene            complement(73378..73830)
                     /locus_tag="IXT20_000414"
     CDS             complement(73378..73830)
                     /locus_tag="IXT20_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YueI family protein"
                     /protein_id="HAQ4858891.1"
                     /translation="MAQDDVKSHLDKGLYGTPLVNPEEQHKYLGTFRERCYLSMTVAE
                     MIQPKNKENFLKELKKHPDASILLNGALPLSVQNQYIQLATKNNSRFTVVNDYVTANP
                     EEFGLLLTAKEAVNEPVIDIEKKYPEENSSPAEKEKPKKKKSFWDRLF"
     gene            73980..74930
                     /locus_tag="IXT20_000415"
     CDS             73980..74930
                     /locus_tag="IXT20_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HAQ4858892.1"
                     /translation="MRYGRLVALLVILSSISLFIGVSNVQLSELLQLDGQSLQILLVT
                     RLPRTICLILVGATSSICGLIMQHLTQNKFVSPTTAGTMDSARLGILVAMIFLPGASL
                     IVRSLTAFCFAFAGTLLFLSLTRLFLQKNQLMLPLIGVMLGNIIGSVATFFAYQFQLV
                     QNMTSWLQGNFATVIRGNYELLYAVVPLLLLVWLFAYRFTIIGLGESFAVNLGIDYQK
                     MQFLGIGLVSLASAVMLIMVGSIPFIGVIIPNLVSRIYGDQVHKTIGVTALAGSLFLV
                     FCDLVARVLIFPYEIPVSVIVGIIGGAIFLYLLIRGRRYA"
     gene            74923..75882
                     /locus_tag="IXT20_000416"
     CDS             74923..75882
                     /locus_tag="IXT20_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HAQ4858893.1"
                     /translation="MLKKKPSIFIGMLVLVLFVTGMYLTIHTYGNWHFACALRSKKIF
                     AFILVSLAVSSATISFQTITHNQFLTPSVLGLESLYVLVQTVLFFFIGGVTMLQQTGT
                     VFFLLNVGLMVGLSLLLFFFLLKKMRANLFLLLMSGMILGTLFGSVSTFLQVIMDPNE
                     YDLLQGRLFASFGNIQSQYLLPASLIIIGCWYLLFRYRHVLDVFHLGNEQAVNLGIDT
                     DRFEFLILCCVSLLTGTSTALVGPVTFLGFIVANISYRVFSTYRHGQLFIGSFLVAML
                     FLTGGQLAVEQIFGWNTTVSVVVEFVGGIYFVTKLIRERKSIL"
     gene            75879..76634
                     /locus_tag="IXT20_000417"
     CDS             75879..76634
                     /locus_tag="IXT20_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ4858894.1"
                     /translation="MIDLQKVNKLYHGKSVVKDVQLTIPEKKLTAFIGPNGAGKSTVL
                     SMISRLIPKDTGEIYLDHNEVKAWKSNELAKKLSILRQSNEINVQITVKELVEFGRFP
                     YTKGKLTTKDEEIVQESLEHLGLEEIADQSIDTLSGGQLQRAYIAMVLAQDTDYILLD
                     EPLNNLDMNFAVQIMQILKNLVQEIGKTIVIVLHDINFAASYADEIVAMKDGQLFTQG
                     TTQEMIKKDVLDELYEMDIRICEIEGRRFCLYF"
     gene            76657..77610
                     /locus_tag="IXT20_000418"
     CDS             76657..77610
                     /locus_tag="IXT20_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HAQ4858895.1"
                     /translation="MKKLVVGILILASFGLAACGNSNNTSQADTKKSSTQTETTLTIT
                     DSNGDQIEVPNNPEKVVVFDNGSLDTMDALGVGDKVVGAATSSLPEYLSSYKKVESAG
                     GIKEPDLEKINQLQPDLIIISGRQRDFQKDLSQIAPTIFLSLDAKNPWESFQQNVTAL
                     GEIFGKQEEAKTQLEELSSAIDQTKKKAEATDKKALVTLVNEGQLSAYGSGSRFGFIH
                     DLFGFEQADDQIEASTHGQSVSYEYVLEKNPDILFVVDRTKAIGGDDSKDDISANELV
                     AQTNAGKNQQIISLEPDVWYLSGGGLESMKLMIEDVNQAFK"
BASE COUNT        25518 a        14034 c        16634 g        21798 t
ORIGIN      
        1 tctctgttcc ctctttatca tggaagtgca aaaagtaata tagggattga taaccttata
       61 gaagttatta ctaataaatt ttattcatca acacatcgag gtccgtctga actttgcgga
      121 aatgttttca aaattgaata tacaaaaaaa agacaacgtc ttgcatatta agtagttcag
      181 ttttggagta caaaaagtga aaatgtaaac ctaaatgagt atcaaattgc aactgaattt
      241 gatactcgtt ttttattgtt aaatcaacgt ttgttgttaa tttacaccac aaaatgtaag
      301 ttaaaatgta tataaaaatg cgaacgtgaa ataattattg taggaatgca aattggtgtg
      361 ttaaaatggt tacaatttac taagcaaaaa ggggtgcatt ttcctatgca atttcttgag
      421 ccaaaaaata agaacgctaa gtctgtggat tgggaaatat cagagcaagt aagggtgata
      481 gttaagcaat acgctgaata tgcagagcgt actgaaagcg aagcagtgga tgaattctta
      541 ttgaacattt tagacgataa gaagtttatt gaatggattg ccaacaagcg aagtaataag
      601 cggattgttg aaaaaatggg tatcaaagat agagtgggtt gatagacaat gaccaagctt
      661 aaaagattag ccacgaagga agatgaaatt gtagatgtta aaattccgat ttcaaacgaa
      721 gaattaaaag atagggctaa acaatatgat ttattaactc caaaacgatt tgcaacaagg
      781 tataacaaaa tgttgttctt gccaacgtct tttaaatgga acggttctga atatcccatt
      841 caatataatt actgcatcaa tccgttttgt tgtaattttg ggaaagaaca gcacaaattc
      901 aaagatgtaa aagggaaacc aagtcgatat aaaatgactg gtagttccaa agataaaggt
      961 cataaaggta tgtactgcaa tgacaatcct attggcagag gtgtatcgca aaactgtaca
     1021 gtaacccctt tatctaattg gtctgttgtt gaagaaatta aacgccttat agaaatcaat
     1081 agcatccaag atgttgagcc agattatcaa tttcataaag aaggctgctc ggaagaagaa
     1141 tcaaccccat tcaatgagcc gaagcaattt tacaaaaggg ggaaaagtag gggcaaatct
     1201 caacgctatc aatgtaaggc gtgtaagaag ttcactaatg ttctaccaaa aagagaagaa
     1261 accactactt atcaccaaca aaagaacacc attcttccaa tgttcgctaa gatggttgtt
     1321 ggaagggttt cagtcagtcg tacttgtgat attttaggaa ttggagttgg aacctactat
     1381 cataagctgg agtggctgta taggcgttgt ttggagtttt tagaacgata tgagacacaa
     1441 ccccttcaaa caaagaaatt taacgagatg tggcttaata cggacaagat gcactactac
     1501 ctaaataacg tccgtaagaa aggacaaggt tcaaagaaat atacggggtt tgaagacctt
     1561 aatatgcaaa cgtatatagt tgtatctgct gaggtcttat caagatatgt gttccgttct
     1621 gatgttgcct atgattggaa tatttcaatg gatgaattaa atgaagatac aagaaaattc
     1681 aaagaagacc atctcaatac ttttagtcgt aaaaatgata ggttagattg gtcttattat
     1741 ccacaagaac catccgctaa cgatagcgaa aataggaatg cttatcttca cgaattaggg
     1801 aaaattacta ataggagtag atttgtcgat ggtttaaatg ttgatgctcc ttatactaca
     1861 actgctcact attggttaat taaacaaatg gttaatgccg acgaatggcg aatgattagt
     1921 gatgatgact tctctattag aaacgccttt tatagagtat tcacgaaaga attaaggttg
     1981 agcgatgcac atcattttat ttgccaagtt aacaagacga aaagcagaaa acaatgctta
     2041 aaagaattcg gacaagcaaa agcagaactg ctcgattggg gagatatcag aggattcaaa
     2101 acaaagtttc tacggacttt ggcatctcat tatctaactg aattactaac aagtcatcaa
     2161 ttccacgaag aggctattag taaagatgga gaaagatatc gcaaatatgc cgataaccct
     2221 atcaaacatc cattagccac taaggataaa ggattttatt cagttgattg tagaacggat
     2281 ttatctgctc tggaaccaaa cgaaatcgca aagatgctat taaatgttaa tgaccattcg
     2341 accaatagct ttattcaaca gataagacgt tatatatcat ctcttgaaag accattaaca
     2401 acagctcgtg gtgataagaa gagctatatt tacgctaact tcaatccgaa gtatgctcaa
     2461 tttgcgatta caattctaag aacttactac aacttttgtc gcccttttaa atcagcggat
     2521 aaaaaggttt tgacacctgc tcaaagatta gggattacag ataaacaatt tgattggaaa
     2581 gatataattt atttcaaatg acaaaaagag cttggagtaa tcccaagctc ttgtgcttta
     2641 tctttactgt tcttcttcaa ttaacgcacc cgttaactca ataagttttt tctttcatat
     2701 acatgaaaat agtagttata cggattttta tcattcttta tccctttttg ggcaaatacc
     2761 tcattccatt cctcataatt cacttctgga aaaaaagtat ctccttcgaa ttcatgatgt
     2821 atttttgtga tgtacatttt ctcaacataa gggaaaaaca aattataaat ctgttctcct
     2881 ccgaaaataa aaatttcttc ttcgttttta cataactcaa aaacatcttc tattgaatga
     2941 acaatttcac aaccattaaa ggtaaacccc ttatctctcg tcagaataat atttcttctg
     3001 tcaggtaagg ctcttccgat tgattcaagg ttcttcctac ctaatattat cggatgtccc
     3061 tttgtagtat ttttaacata ttcccagtcc ttgggaatcc tccaaggaat gtcattctct
     3121 ttgccaatca ctctattctt atccatcgca gcaatcaaag aaactttcat ttataacaca
     3181 gcctttttaa ctaatttacc atctaatttc ttatttctat caaaaaacct tttctcatta
     3241 tcctgcccgt tagttgaata agcaattttt tctataagtt atattatcat aataccacaa
     3301 aatcacaaat tttcattcaa ttatcatttt caattgcatt ttataaatca ttttgtatgt
     3361 aatttggtat gtaagttaaa atgaaaaaag tagtccattt tgaagtacat tttgtcattt
     3421 tgtactccaa aactgaacta cttacttgca tatatacgcc tttatagtgg agtactacat
     3481 ttacgagatt cggttagagt atcagaaaaa gaaaaaataa aagttacaga aatgtatact
     3541 tcaataaatg gtgaattatg taagattgat agagcttatt ctggagaaat tgttattttg
     3601 caaaatgagt ttttgaagtt aaatagtgtt cttggagata caaaactatt gccacagaga
     3661 aaaaagattg aaaatccgca ccctctacta caaacaactg ttgaaccgag taaacctgaa
     3721 cagagagaaa tgttgcttga tgcccttttg gaaatctcag atagtgatcc gcttctacga
     3781 tattacgtgg attctacgac acatgaaatt atactttctt tcttagggaa agtacaaatg
     3841 gaagtgatta gtgcactgtt gcaagaaaag tatcatgtgg agatagaact aaaagagcct
     3901 acagtcattt atatggagag accgttaaaa aatgcagaat ataccattca catcgaagtg
     3961 ccgccaaatc ctttctgggc ttccattggt ttatctgtat caccgcttcc gttgggaagt
     4021 ggaatgcagt atgagagctc ggtttctctt ggatacttaa atcaatcatt tcaaaatgca
     4081 gttatggaag ggatacgcta tggttgcgaa caaggattat atggttggaa tgtgacggat
     4141 tgtaaaatct gttttaagta cggtttatac tatagccctg ttagtactcc agcagatttt
     4201 cggatgctta ctcctattgt actggagcaa gcctttagaa aagctggaac agaattgtta
     4261 gagccatatc ttagttttaa agtttatgca ccacaggaat atctttcacg ggcatataac
     4321 gatgctccca aatattgtgc aaatatcgta aatactcaac tgaaaaataa tgaggtcatt
     4381 attattggag aaattcctgc tcgatgtatt caagattatc gcaatgattt aacttttttt
     4441 acaaatgggc ttagtgtttg tttagcagag ctaaaaggat atcaggttac cactggcgaa
     4501 cctgtttgcc agacccgtcg tctaaatagt cggatagata aagtaagata tatgttcaat
     4561 aaaataactt agtgcgtttt atgttgttat ataaatatgg tttcttatta aataagatga
     4621 aatattcttt aatatagatt tgaattaaag tggaaaggag gagattgtta ttataaacta
     4681 caagtggata ttgtgtccta tttgtggaaa taaaacaaga ctacgaatac gagtggatac
     4741 tatacttaaa aatttccctt tatactgccc caaatgtaag aacgaaactt taattaatgt
     4801 tcaaaaaatg aatataataa caatcaaaga gccagacgcc aagacgcaga gccgataatt
     4861 tgagaaatga aactctcatc ttatcggctc tttttgttta tctgaatttt actgactagc
     4921 cttcaatatt tcttgcttca ttgatacctt ttgctagtgc ttccattaag gatagttctt
     4981 tgtctgtaaa gctatccatg tatttttcta cctgtaacct tcgagtgctt ttaatcaaat
     5041 tatctgtagg taagaaaaat tcatcaatag atacatggag taatgataca agatcataca
     5101 gcacttgtgt acttgggtgt tgtcctttgt tttctatatt tgttaaataa cgtgggtcaa
     5161 tttcaatcat tgttcccact tgttcacgag tgagaccccg tttcattcga gcctctttaa
     5221 tcgctaaacc aacggctcta aaatcgtatt tatcttcttt tttacgcata ctaaatcacc
     5281 tctatacatt ttactgttcc tatataacca taaacaggta tagaaaaaca tgttatatag
     5341 tttatgagtt cctatagaac actgtggaat ttattaatgc gatgaatcca atttcaaata
     5401 gaaaggtcgc tgattttatt tacctataca actttataaa atttagtcat actcctagaa
     5461 agtagataaa attggcgact taatggaggg aaaagtcgcc atgagaaaga taatctgtca
     5521 tctagttctc ttaattaagt tcttatcaaa aaaacttatc cacctgtact ggatagatat
     5581 gaccatcaat gtggttttgc agattacata gatagaaaaa tcaaagcata tgaacagaga
     5641 aaacaatctg tttatgtgct tttttggtca cttaataaat atcttttagg atttctcttc
     5701 atttatgcaa caaatccccc gttcgtgtcg gggtttagta tgaagggaga taaagaagca
     5761 catgccactc cctttcacga cagaaagggg gtgaaaagca tgaaaccatc ttcttttcaa
     5821 gaaaccatag aaaatcagtt tgactatatc tgcaaacaag ttattgaaga tgaacgaaaa
     5881 gattacttca aacatctaga cagactatca gcaaaagaag tctcgttttc tgatatagat
     5941 aactaccttg tcaactcttt ttctactgtt gatacgtacg tcactgattt ccaactattt
     6001 aaattatttg gattaagaat caatgttgaa agtgatttat taagtgaagc cctgcgtcat
     6061 ctttcagaga aaaaacggaa cattatttta cttcattact ttatggatat gagtgatgtg
     6121 gagattgcgg agttactaaa tttgaatcgt tcgactgttt atcgtcatcg aataagtgga
     6181 ctagccatga ttaaagaatt catgaaggag tgtaaagaat gaaaacagct tatccaaggg
     6241 ttcccttccc cctcattgta aaggcgacag atggcgacgt ggaagcaatt aatcagattg
     6301 ttaaacatta tcgagggtat acctcaaaac gttcattacg tcgcatgaca gacgaatatg
     6361 gaaattctca tatggttatt gatgaaaccc tacgtggcag aatggaaacg aaactcatct
     6421 ctaagatttt atcgttcgaa attaaatagt cccatttttt cgtggaagcg tgtcgaatcg
     6481 tatccacgct tcccgatttg ggaggtttgt tattccatca aagcatattg tgtgttcagt
     6541 aggttttgat aggctaacaa gccaaacgtt ctttgacaac caaataaagg caatcgaata
     6601 cgtttcggta agaaaagagc tgatgaacta acacgccatg acctacaaaa ttagtgagcg
     6661 attcatgtta gtaattcgaa agtaatcttt agcaggattg ctgccatgac tttcttatcg
     6721 tgataatgat actcccgtat agccatagtc cgagcgttag aagcgtcaca ggcaatgggt
     6781 acggctacat gagaaccatg cagaagtgaa attcctgtga gctttgctag agctgttcga
     6841 ttgctaagat attcttctct tgtcagtgta ttattataaa actatttgat ataattatag
     6901 gtgaatattg gagttttcct agcctataat tgggaaagga gtaataatga taacggtcac
     6961 aaaaaaggat ttagttgagc taggatatgg aacttctttt tcagcagaca tcataaaaaa
     7021 agcaaaagaa ttgatgatta caaaagggca tatctactat caatctcgaa agctagatag
     7081 agtccctcgt gaagctgttg aagaaattct aggaattcgg ttttcaaatg aaaacaggtc
     7141 tgagtagttt ttgcacagta aggagtaaaa actatggtta aagacccaat aaaaaaagca
     7201 aagaatggaa cctattattt tcgtgcaaat ctaggttatg attctaaagg gaaaaaaatt
     7261 caaaaatatc gtagtggatt cactactaaa aaagaagcaa gagaagaata ttcgaaactg
     7321 ctattgatga aaccagaaga attaagtgag aataaggata aaatgacgtt taaacattat
     7381 atctttgaaa ttttccttcc atggtataag acacaggtaa agctcagaac atatgaaaat
     7441 cgcttaccaa ctattaagaa gcatttctct tattttgata aaatggctgt ttccgatatt
     7501 gagcctattg atgtgcaaaa ttggcaacta aaattatcaa agaagtgtaa gtcttcttat
     7561 gtaagagctg ttcaaggact attttcagtt gctatggatc gagcaattgt ccttgggata
     7621 accaccacaa atccatctaa gattattggc aatgtgaaaa agcaaaagtc taaaattgaa
     7681 ttttggacaa aagaagaatt tgaaaaagta atctctctaa tttacaaaga agattactat
     7741 caacactttt tatttatctc tctgtggttt ttatttatga ctggaatgcg aattggtgaa
     7801 gctactgcca ttcaatggga agatattgat tttgactcag gagtactaac catcaacaaa
     7861 acactctatt acaagaatca atcgaattat agatttgttg aaccaaaaac aaaggcgagc
     7921 gttcgtcata ttgtattaga taaatgcaca ttaacttatt tatccgaatg gaaggaagta
     7981 caacaaaatc taattcagac agactttgta atgagctata acggtattcc tacccaaaaa
     8041 catacactat catatgctat tgaacgctat gctaagctgg ctggtatcca tcgaatcaga
     8101 atccatgggt tacggcattc tcatgcttcc ttgttaatca gtatgggaga aaatccactt
     8161 atcattaaag atcgtctagg acacgaagat attgagacga ctttaggaac gtatggacat
     8221 ctttatccta acagtaattt cgaggtagct aacaaattaa atggagccat ttcctttaaa
     8281 gaagcaaaac aaaattatga tacttcacca aagaatcaat ttactgtaga ctacttgcga
     8341 aacaaagaaa tgaaaaaagt gcaataaaag tgcagtaaaa agataagaag agccagaaac
     8401 ccttggtata agaggatttc tagctctcct acaactattc ccactccact gttgctggtg
     8461 gtttagaagt gatgtcatac acgatacggt tgacgtgggc tacttcgttt acgatacgta
     8521 cagagatttt ttgcaagact tcccatggga tacgggcaaa gtcagcggtc attccgtcga
     8581 ttgaagttac agcacggata ccgactgtgt agtcgtaagt acgtccgtca cccataacac
     8641 cgacagagcg gatccctgga agaacagtga aatattgcca gatatcacgg tcaagtccag
     8701 cagctgcgat ttcttcacgc aagatagcat cagattcgcg tacgatttct agcttgtctt
     8761 cagtgatttc gccaagaaca cgaataccaa gaccaggacc agggaatggc tgacgccaaa
     8821 cgattgaatc aggcatgcct aactgagtcc ctaattcacg gacttcgtct ttgaataatg
     8881 tgtttaatgg ttcgatcaat tcaaattgca tatcttctgg cagtccacct acattatggt
     8941 gagatttgat cgtctgtgct gtttctgtac cagattcgat aacgtcagta tacaacgttc
     9001 cttgtgctag gaaagagacg ccttcttcac cagcaagttt tgttgcttcg tcatcaaaaa
     9061 catatacgaa ttcattccca atgattttac gtttttgttc tgggtcagat acacctttca
     9121 acttgtctaa gaagcgatct cttgcatcga ctttgatgat gttcaagccg aattttccgc
     9181 ctagactttc cattacttgt tccgcttcgc ctttgcgtag cagaccgtgg tcaacaaaga
     9241 tagatgtcaa ttggtcaccg atcgcttttt gtaaaagaac cccaacgaca ctagaatcaa
     9301 cgccacctga aagaccaagc aagacttttt tgtcaccaac ttgttcacgg attttttcta
     9361 cttgcatatc gataaagtta tccattgtcc agttaccttc acagtgacaa acatcaaagg
     9421 caaagtggcg cagcaattcg attccgtatt cggaatgacg tacttctgca tggaattgga
     9481 taccataaaa attacgtgtt gtgtcttgga tagaagcaat cggacaatct ttgcttgttc
     9541 cgactgtttc aaagccttca ggaactttag ttaccaagtc accatggctc atccatactg
     9601 tttgttgttt tggtgtttct ttaaacaaga cagcttgatc gcttgttact tctaacaagg
     9661 ctttcccata ttcgcggttt ttagcaggct ctacggaacc gccaagtttg tacgtgatca
     9721 attgcatacc atagcaaatc cctaatacag gaatacctaa attgaagatc tcaggatcga
     9781 tcccaaatgc attctcatca tatacactgt ttggtccgcc agaaagaatg attccctttg
     9841 gattcatcgc ttttacttct tctgcagtaa tgcggtggct caataattcg gaaaagactc
     9901 caagatcacg aatacgtctt gtgatcaatt ggttatactg actaccgtag tctaatacga
     9961 taatcttttc aacgttcgtc atatcggcaa cgtttgtcaa atttattacc cctatccctt
    10021 taaattttat ctcaaaatag tcaaactatt ttaaaatgct aatattttcg catgaaaatc
    10081 gaatcgatga tatgattttc agttttatgg agaattatat cggctcttcc tcgtgtaggc
    10141 aagatatatt cctgtaaatt tttcaaattc acatttttcc atacatcttt tgccatttta
    10201 aaggcttctt cacgatctcc gatagcaaaa cgataataat aattctctgg attttggaaa
    10261 gccgtatcca gtaacgcacc aaaacgatcc aaataccatt tttcgatcag cacgggatcg
    10321 gcatcgacat agacagaaaa atcaaaaaag tcgctgacgt agatttgctg attcgcaggt
    10381 aattgaagcg tattgatccc ctcaacgatc aagatatcag gctgctggat caattcgtac
    10441 ttgtctggaa tgatatcata cacatcatgt gagtaaacag gtacttttat ctcttcctga
    10501 ccagatttta cacggttcaa gaaatcgatc agcatctcca tatcataact ttcaggaaat
    10561 cctttacggt ccatgattcc acgctctttt aatatacgat ttggataaag aaatccgtca
    10621 gtcgtgatca gctgaacgtt tcttcgtttg aaggtacgag acaaaatcgt ctgcagcagt
    10681 ctggctgttg tactttttcc aacggctaca cttccggcga tcccgataat aaaaggcggt
    10741 acttctacgt atttatgaag aaataaacct ttactcaaga acaaagattc aaattctttc
    10801 atataaagat aaatcagatg agcaagaggt acatagatat ctcgaacatc ctgcatggaa
    10861 atgcggtcat tcaaactttt gatattatct agttcatctt gtgtcaaagg tgcttgccca
    10921 ttacgataaa attctcgcca ttcttctctt gccacacggt agtagttcat tggatcattc
    10981 atattagcct cctgcgttct cccatcaatg gaactatttt aacacaaaat aaacgcaaac
    11041 cccaccactt aagagagatt gccgatcttt ttgtataatc tttataatag aaagaaacgg
    11101 agtcttattc ttgacttgcg cacggataca taaaaggata gactgttgtc atgaaagaaa
    11161 tgagaaggat ggaagacatg acaaatcatt actattctga acgcccagat acggcgcacg
    11221 attttgaaca atgggctttt gagttaaaag gcagaacctt tcaatttgta acagattctg
    11281 gtgtattttc acgtgaaaca gtggattatg gttcacgtgt cctgattgat gctttcgatt
    11341 gggaagagtt acctgatgaa ggaaagatct tagatgtggg atgcgggtat ggaccgattg
    11401 ggttagcttt ggcatttgcg acacagcggt ttgttgaaat ggtcgacatt aatgaacgag
    11461 cggtaagtct agcgcaagga aacgcaaagc gtaaccaaat cgaacatgtt gatatccatc
    11521 aatcgaatat ctatgaagca gttcatgaag aaacctatgc agctatcgta agcaatccgc
    11581 cgatccgtgc tggaaaaaaa gttgtacatg aaattttgac aggcgcatat cctcggttga
    11641 agaagggcgg tacactgaca attgtgatcc agaagaaaca aggagcgccg agtgcgcaaa
    11701 agaaaatgga agaaacattt ggtaatgcag aaatcgtcac aaaagataaa ggatattata
    11761 tcatcaaaag tgtgaaagaa taactagctg aaaaatagtg gagcgacgaa agagtggaac
    11821 tgggcgtgca tgtacgggta cgtatcagtt ctttttttgt tgagaagttt ttaaaaacaa
    11881 agaactattt tttcttcaaa aagaaatagt aggaacagag tcgttgtttt ttacgataaa
    11941 ataaataaga tacagaactt gtatagaagg ctgccaagtc ggtagataaa ggaggttttt
    12001 atcaaaaaac atactttcct tttttctcta ttcttttctt gggggttttg gtacactgat
    12061 agggaattta gatagaaggg agccaatagg atgaagctga ccatcagaga tatcgcagaa
    12121 atggcaggcg tatcagtaac aactgtctca cagattttga ataacaaagg aagcagattt
    12181 agtgataaga caagaaaaaa agtgttgtcg atcgtgaatg aacatcatta taagcccgat
    12241 tatttcgctt caaatgtgat caatcgacat tcaaagacca tcggaatgat cgtccctgat
    12301 gtcactgatt tcttcttctc caaactgatc gaaggtgtag agagctattt gaatcctttg
    12361 ggctatgtga tcatgttatg caattctcgt catagccaag aaaatgagat caagtattta
    12421 caagagcttt cccaccgctc agtggacgga atcttgctgg ccacaccaaa tattttacct
    12481 gagaaatatg cattagacag cggtttttat caaaaaatgc caatcatttt aatcgatcgc
    12541 ggattgaatc gtcgggataa cggacggctt atcgtaaagg aatatgaggg cgcctatcaa
    12601 gctgtttcgc tgtttattga aaatggccat acgaagatcg gcatgctgaa ggaaaccact
    12661 ggatactatc aattagaaga acgtttcaac ggatatcgtc atgcgctgaa agatcatggc
    12721 ctgcctttta atgggaaatt tgttgaacat ggagagttga ctgtccaagg tggatatact
    12781 gcatcaaaaa aattactcaa acataaagat atcacggcta ttttttgcgg gaatgatgcc
    12841 atggcgatcg gatgctatca ggcgatcgat gaattgggca aaaaaatccc agaagatatc
    12901 tcagtcatag gattcgatgg attaaagctg tcagaataca tgattccgca attgacgaca
    12961 gtgcagcagc caagttttga tattggattt tatgctgcac gtttcctgat cgatacgatc
    13021 gaatttcctc aaagaaaagt tccgaacaaa gtgttcgaaa caaaattaat tattcgtgag
    13081 agcgtaaaag tattgacaaa gggctaactc ggcggtatat tgcttatgta agcatttaca
    13141 aaggctagtt agtgcttttt ttatttttag taaaccagtt tactaaagtg gtttaccttt
    13201 cctaaaaata atgtataata ccgaatgaag acaaggagga tcaggcaatg cgaatggtgg
    13261 atctgattga aaaaaagagg gatggaaacg agctgtcgaa agaggaaatc gagtacatcg
    13321 tcacgaacta tacgaatgga aagattccag attatcaagt cagtgcatta ttgatggcaa
    13381 ttttttatca agacatgaca aatgaagaaa tcacgaactt aacattggcg attgctaatt
    13441 ctggtgatgt catcgatttg agttctcttg aaggaataaa ggtagataag cattcgacag
    13501 gtggcgttgg tgacacgacc acattgattt tagcccccct agtagctagt gtcggagtga
    13561 cagtggctaa aatgtctgga cgcggtctag gttacacagg tggtacacta gacaagctag
    13621 aagcaattcc cggatttcag atcgagcttt cggatgaaga atttgtacgc attgttaatg
    13681 aaagtaaagt agcggtaatt ggtcaatcag gaaacttggc accggcggac aaaaaattgt
    13741 atgccttgcg tgacgtcaca gcgaccgttg attctcttcc gctgattgcc agttcgatta
    13801 tgagtaaaaa aatcgcggca ggagcagatg caatcgtttt ggatgtgacc actggagacg
    13861 gtgctttcat gaagaacatc gaagatgcaa gacgtctggc aaagaccatg acgagcattg
    13921 ggaaactggc taaccgagaa acagtggcgg ttatctcaga tatgtcagaa cctcttggcg
    13981 aagctatcgg aaatagtcta gaagttgtcg aggcaatcga aacattacaa ggaaatggtc
    14041 cagaagatct agtagaaatg tgctacgcct taggaagcca aatggttgtt cttgctggca
    14101 aagctaagac aaccgatgaa gcaagaacgt tgcttcaaga agctctggaa tcaggtaaag
    14161 cgttagcaaa attcaaagaa atgatccaaa atcaaggggg agacccagcg atcgtggaac
    14221 atccagaacg aattttgact gcacgctaca caatggagct tcctgctaaa caaagcggag
    14281 ttgtgtcaaa aatcgtggca aatgaattag gaattgctgc aatgatgcta ggtgctgggc
    14341 gtaaaaccaa agaagaagat attgatcacg ctgttggatt gaaactgcat aaaaaaattg
    14401 gtgacactgt gacaaaaggt gagtcactat tgacgatcta tagtaacgac gaagaaatca
    14461 cttctgtcat tgaattatta tataaaaata ttgaaattgg tgaatcggca atgaagccaa
    14521 cgttgattca tgacatcatc acagaataaa ggaaaaaagc ggactttacc gttttaaaga
    14581 aaaggggaaa agaaatggaa ctaaaccgaa tgatcgacca tactattttg aaagcagacg
    14641 catctaaaga agctgtgatg caaattatcg aagaagcaaa aaaataccat ttttattctg
    14701 tctgcatcaa tccagcatgg gttgcgctag cgaaagaaca attacaaggt gagccagtag
    14761 ccgtatgtac agtaatcggg tttcctctag gagcaaacac acccgagaca aaagcatttg
    14821 aaacaacaga tgccatcaat aacggtgcag atgaagtcga tatggtcatc aatatcggtg
    14881 ctttgaaatc aaaggatgat caacaagtcc aaaaagatat cgaagcagtt gttgaagcag
    14941 caaaagatcg tgcgcttgtc aaagttatta ttgaaacatc gttattagat agagaagaaa
    15001 tcatccgagc ttgcgaaata gcaaaagctg caggcgctga ttttgtcaaa acatcaactg
    15061 gtttctcaac aggcggagca aaggtagagg atgtccgttt gatgcgtgaa actgtaggac
    15121 cagaaatggg tgtcaaagct tccggcggta tccataacga agaagaagca atggcaatga
    15181 tcgaagctgg tgctacaaga atcggaacaa gcgcaggagt agcaatcgta tctggttcaa
    15241 ctggtgaagg ctattaattt tttcaaagga gaagctcatg gcagaactca aacaagaatg
    15301 gatcaacaca gctattgaag cgttagataa agcctatgtt ccttattccc attttccggt
    15361 aggcgcttgt ctcgtaacgg aatcaggcaa gatttatcaa ggcatcaaca ttgagaatgc
    15421 atcatttgga ttaacgaatt gtgccgagcg cacagctttt ttcaaagcag tatctgaagg
    15481 agaaaaaaca tttacgcatc tagtcgtagc ggggcacacc cccgatccta tctctccttg
    15541 tggtgcatgt cgacaagtaa tggcagaatt ctgttcccct gatatgccgg ttacgctagt
    15601 aggagacaat ggtgtgacga aggcaacgac cgttcgtgca ctgctgcctt atgcatttac
    15661 tgaaaaagat ctataactca tgttcattca gtcagctgac tgttgaaaaa taaaacaatt
    15721 attttaggag gctttacaga aatgaaaaaa gcaaaattat ttggtttagg tgctgtcgca
    15781 ctagcagcag ggttattttt aggtgcatgc ggcaacaacg gctctactga ttcatccggt
    15841 ggcaaaagct catcagacac aacaactgca gcattgatca cagacacagg tggtgtagac
    15901 gaccgttcat tcaaccagtc agcttgggaa ggactagaaa aatgggggaa agatcaagga
    15961 ctttcacgag gaaatgatgg cttccaatat ttccaatctt caaatgaatc agattacatc
    16021 ccaaatattg accaagcatt gaatgctgga ttcaaaacga tttttggtat tggttataaa
    16081 ctaaaaccag cgatccaaga acaagcaaca aacaatacag gtacgaactt tgttattatc
    16141 gatgatgtca tcgatggatt agataacgtt gtatctgcaa ctttcaaaga caacgaagct
    16201 tcttatcttg caggtgttgc agcagcttat acgactgaaa caaacgtagt cggctttatc
    16261 ggcggtgtaa aaggtgaagt aatcgatcgt tttgatgctg gattcaaagc cggtgttgat
    16321 gcaggtgcga aagaattagg aaaagaaatc aaagtattga atcaatatgc tggtgacttc
    16381 tctgctccag ataaaggacg ttcaattgct caaggtatgt atgcacaaaa cgcagatatc
    16441 atcttccatg cttcaggcgg tacaggtaac ggtgtattcc aagaagcaaa atcattgaac
    16501 gaatcaggcg acaaaaaagt ttgggttatc ggtgttgacc gcgaccaatc agacgaaggc
    16561 gaatacacat taaacggcga aaagaaaaac ttcacattga catctacact gaaagcagtt
    16621 ggaacagtag tagaagattt ggcacaaaaa tcagcggacg gtaaattccc tggcggcgaa
    16681 cacactgttt atggattgaa agaagacggt gttggtctaa cagaaggaca actttctgac
    16741 gaagcgaaaa aagcagttga tgaagcaaaa gaaaaaatca tttcaggtga cgtaaaagtt
    16801 ccagaaactc ctgaagaaaa ctaaaaccat ttctatagcc ttgttagaca catgtttgac
    16861 aaggctatgg tttgattaga acaagaattg ttcatttatc caagctgcct tgtgacagtt
    16921 tggataaggg aacaactccc tgaagtaatg tttgtttcaa aagaagaacg ctcactaagc
    16981 gtgaatctta gtcaatcgtt gccctagata gaaatcgtta ggatgtgaaa aaaaggtggt
    17041 tcaagaaaat attgtcatag aaatgcagaa tattaccaaa gaatttggaa cattcaaggc
    17101 aaatgacaac atcaatttac aagtaaaggc aggagaaatt catgcgttgc ttggagaaaa
    17161 cggtgctggc aaatctacat tgatgaacgt gctttccgga ttattagagc cgacatcagg
    17221 gaaaattttg atgcgtggga aagaagtaaa gatcacaagt ccgaccaaag ccaaccaatt
    17281 agggattggg atggtccatc agcactttat gcttgttgat gcctttactg taacagaaaa
    17341 catcgtgttg ggaagcgagc ctagtcgcgc agggatgctt gaccataaaa aagcacgaaa
    17401 agagatccaa aaagtttctg aacagtatgg attgtcagtc aacccagatg cttacgttcg
    17461 tgatatttca gttggaatgg aacaacgggt agaaatttta aaaacacttt accgaggagc
    17521 agatgtactg atttttgatg agccgacagc tgtattgacc cctcaagaaa ttgatgaatt
    17581 aatcgtgatc atgaaggaat tagtcaaaga aggcaagtcg atcattttga ttacgcataa
    17641 gttagatgaa atcaaagcag tagctgaccg ctgtacagtt atccgccgtg gaaaaggaat
    17701 cggtacagtc aacgttaaag acgtaacctc acaacaatta gccgatatga tggttggaag
    17761 aacggtttca ttcaaaacga tgaaaaaaga agcgaagcct caagaagtcg ttttgtctat
    17821 tgaaaatcta gtggtgaaag aaaatcgtgg attagaagcc gtgaaaaact tgaacttaga
    17881 ggttcgtgct ggcgaagtac ttggtatcgc tggaatcgat ggaaacgggc agtcggagtt
    17941 gatccaagct ttgactggtt tgcgaaaggc agaaagcgga catatcaagc taaaagggga
    18001 agacatcact aataaaaaac cccgaaaaat cactgaacat ggtgtaggac acgtgccaga
    18061 agaccgtcat aaatacgggt tagtcctaga tatgacatta tctgaaaata tcgcactaca
    18121 aacctatcat caaaaacctt acagtaaaaa cggcatgctg aattattcag taatgaatga
    18181 acatgccaga gaattgatcg aagaatatga tgttcgaaca acgaatgaac ttgttcccgc
    18241 aaaagcttta tcaggcggaa atcagcaaaa agcaattatc gctcgtgaag tcgaccgaga
    18301 tcctgatctg ttgattgttg caaatccaac tcgtggactg gatgtaggtg cgatcgaatt
    18361 tattcataaa cgtctgatcg aacaaaggga caaatacaaa gcagtgttac tgatcagttt
    18421 cgaattagaa gaaattttaa atgtttcgga tcgtattgct gttatccatg aaggagaaat
    18481 tgtcggtatc gttgatccga aagaaacatc tgaaaatgaa ttaggtctat tgatggcggg
    18541 ctatacctta gaagaagcac gaaaagagtt agaaaagggg gctggcgcac atgaatgatc
    18601 gcagcgaaaa attccgtaat ttattagtgc ctgtactttc agttgtttta ggattgatct
    18661 taggcgcgat attgatggct gcttttggtt ttcagcccat ccaaggttat tcttctatgc
    18721 tagatgcagc ccttggttcg caaagaggga taggtgaaac gttaaggcaa gcaacgccgt
    18781 tgatttttac agcattaggt ttttctgtag ctaactcagc tgggtttttc aatattgggc
    18841 tttctggaca agcactttgc gggtggatcg tcagtgtctg ggtggcactt gcgtttccag
    18901 atctgccaaa agttgttttg cttccgttat gcgtgatctt aggtgcctta gctggtgcga
    18961 tggcagcggc tgttccaggt ttacttcgag cattttttgg aacaagtgaa gtgatcgtaa
    19021 caatcatgat gaactatatc ttattatacg taagcacatt tgctttacat gaagtgatgc
    19081 ctgaaagcta tcgctcaagt ttggactctt ccaatatgat cagtcaaaat gcctcactgc
    19141 ggttaccatg gttaacacaa gcttttggcg gctctcgcgt aaactcagga ttatttttag
    19201 caatcatcgc gcttattttc gtttggctgc tgatgaagaa aacgacttta ggatttgaaa
    19261 tccgttctgt tggattgaat ccttttgctt ctgagtatgc aggaatgagt agtaaacgta
    19321 cgatcgtttt gtcgatggtc atttctggcg ctttggcagg gcttggcggt gtggttgaag
    19381 gattaggaac gtatcaaaac ttctttgttc aaacgacttc gctatctatt ggatttgacg
    19441 gaatggctgt ttccttgctt ggttctggat cagcaatcgg tattctttta tcagcgatct
    19501 tgttcagtat tttgaagatc ggtggacttg gcatgcagac aggagcaggt gttccgtttg
    19561 agatcgtcaa tgtatcgatt gctttgatta tcttctttgt cgggatcaac ttcctgattc
    19621 gtttcgtcat ggctaaagtc ttccaaggga aaaaacaaga agagatcgtt gcgacaatag
    19681 aatcgaaacc aacgaatcaa aaccatgaag ggggcgaact ataatgtcga ctatcgaatt
    19741 aattgccttg atccttactt ctacactggt ttattcaaca ccgctaattc taacgtcact
    19801 aggcggaact ttttctgaac gaagcgggat cgtaaacgta gggctagagg gaatcatggt
    19861 catgggtgct tttagttcca tcgttttcaa cttgacgatg agtccatcac ttggttctat
    19921 gacaccatgg atcgctattt tagtgggtgg cgtcgtaggt atcctatttt ctctgattca
    19981 cgcagtagca acagtcagtt tgcgtgctga ccatattatc agcgggacag taattaatct
    20041 gatggcacct gctttaggtg ttttcttgat caaagcatgg tatggtaaag gtcaaactga
    20101 taacatcacg cagaatctgg ggtatttttc tttcccagga ttagcagata ttccggtcat
    20161 tggaccaatt ttctttaaaa atacattctt acctgcttat tgtgctattt tgattgctgt
    20221 cctagcttgg gtgatcatct tcaagacgaa attcggcttg cgcctccgtt cagttggtga
    20281 aaatcctcaa gctgccgaca cattaggtat caatgtatat gctatgcgtt attcaggcgt
    20341 actgatttct ggcttgttag gcggtatggg cggtgctgtt tttgctcagt cgatttccgg
    20401 taacttttca gctggtacga tcgttggaca agggttcatc tccatggctg ctatgatctt
    20461 tggtaagtgg aatcctttag gggcaatggg tgcctcattg ttctttggtt ttgctcaaag
    20521 cttgagtatc attggcgcac aattaccagt tatttcttcg attccgccag tattgctgca
    20581 aattgctcca tatttgctga caatcattgt gctggttgtt ttcttaggga aagcttctgg
    20641 tccaaaagca aacgggaaga actatatcaa atcaaaataa tcttttagcg aataaaaagg
    20701 gtgggttacg ggaagtgttt ttcgtctcgc cctttttgtt ttaaaggcaa taaaaataaa
    20761 aagaaagaag gaataatcat gtttaaacgc gtacatctga tcgtcatgga ttcagtagga
    20821 attggagaag caccagatgc cgataagttc ggtgatgttg gttctgatac attaggacac
    20881 attgcgaaag aagcaggatt gaacgttcca catttagagc agcttggttt aggaacgatc
    20941 cgtcctcttg aaggtgtgga atcagtgaaa gatcatcaag gctatgcgac gaaattagaa
    21001 gaagtttctg tagggaaaga tacgatgaca ggccactggg aaatcatggg attgaatatc
    21061 aaaactccat ttcgtgtgtt tcctaatgga ttccctgatg atctgatcaa acaaatcgaa
    21121 gaattttctg gccgtaaagt cgtatgtaac aaaccttata gcggaacagc tgttattgac
    21181 gattacggtg aacaccaaat gaaaacaggc gatttaattg tatatacttc tgccgatcct
    21241 gtccttcaga tcgcggcaca tgaagaaatc attcctttag aagaattata tcggatttgc
    21301 caattcgttc gtgatataac aaaagatgat ccgtatatga ttggccgaat cattgcacgt
    21361 ccgtacgtgg gtgaaccagg agacttcaca cgaacaagca atcgccatga ttatgcgttg
    21421 aatccattcg gacacacagt ccttgattct ttgaaagaag caggaaagga cgtgattgct
    21481 gttggaaaga tcaacgatat tttcaacgga caaggaatca ctgaatctgt ccgcacgaaa
    21541 agcaatatgg atggcgttga ccagctgctg aaagttatga aaaacgattt ttcaggaatc
    21601 agctttacca atttagtaga ttttgatgca ttatacggtc atcgtcggga tgtagtcggc
    21661 tatgcgcatg cgattgaaga atttgactta cgaattccag aattgcttga agcgatggaa
    21721 gaagacgact tgctaatgat tacagccgat cacggaaacg atccgacgtt tactggtacc
    21781 gatcatacgc gagaatatgt accgttattg gtttacagta aaaaaatgaa agaacaaggg
    21841 gcattgcctc aaggatatta ttcagatatt tcagcaacga ttgctgagaa ctttagtgtt
    21901 tcttcgactg aaaatggcga aagcttcttg aaattattaa aataatcttt ttaaatagga
    21961 gaaagcaaat gaccaagtta agcgagatgt tacaagaaac gacccagttt atccttgata
    22021 aaggagtaaa agaaattgat tttggtttga ttctaggttc aggattaggt gaattagcag
    22081 atgaaatcga agatgctgta atgatttctt atgatgagat cccgttcttt cctgtttcga
    22141 cagtagttgg gcatgcagga aagctagttt atgggtctct ggcaggcaag aaagtgttag
    22201 ccatgcaagg gagattccac tactatgaag gtcattccat gcaaacagtg acctatccag
    22261 tacgtgtgat ggccggacta ggggcccatt cagtcattgt gactaatgca tgtggtggtg
    22321 tcaacgaatc atttgtacca ggagatttga tgctgattac cgatcatatc aattttactg
    22381 gtgaaaatcc tttgatcggt ccgaatgaag atgcaatggg ccctagattt ccagatatga
    22441 gtcaagctta tacgcctgac tatcaagcaa cagcaaaagc agcagcagaa aaactaggtt
    22501 tggatcttaa agaaggcgta tatatgggct attctggacc aacttatgaa acgcctgcgg
    22561 agatccgaat gtctcggaca ataggagccg atgcagtagg gatgtcgact gttccagaag
    22621 tcatcgtagc agctcacagc ggattaaaag tgttaggaat ctcttgtatt accaacttag
    22681 cagcaggtat gcaagcgaat ctgagccatg aagaagttgt agaaacaact cagagagtca
    22741 aacaatcttt taaagcattg gttaaagaaa cattgacatt attgtaatag gaggaacaat
    22801 aaaatgagtg tgcatattga agcgaaagaa ggcgagatcg ccgataagat tttacttccg
    22861 ggagatcctt tgcgagctaa atatattgcg gagacttttt tggaagatcc agtttgttac
    22921 aatcaggtac gcggtatgtt aggctttacc ggcaaatata aaggagaacg tatttctgtt
    22981 caaggaacag gaatgggaat gccatcagcc accatctatg cccatgaatt gatccagtcg
    23041 tatggtgtaa agaaattgat tcgagtaggt acatgcggtg cattgtcaaa agacgtccat
    23101 gttcgtgatt tagtactagc acaaggtgca gcgaccagtt cttcgatgat cgaaaaaaat
    23161 ttccaagcct ttcattttcc tccaatcagt gatttcaatc tattactgaa agcatatgag
    23221 atagctaaag aaaagggcta tacggttcat gtaggaaatg ttctttcgga agattctttc
    23281 tacaaagatg atttgacaga gacgttccaa ttagctgaac ttggcgtttt aggagtggaa
    23341 atggaagcag cggctcttta ttacttaggc gcgaaatacc atgtgcagac attaagtttg
    23401 atgaccgtta gcgaccattt gatcaccggt gaagaaacaa cagctgcaga acgtcaatct
    23461 acgttcaatg aaatgatcga agtaggattg gaaacagcga tagcagaata agaaggaaat
    23521 gaaaaagctg ccctgcatta agtaataaga acatccaagc aaaaatagct cctcatgttt
    23581 ttgacttggc tggacttatt attgcaaatg taagcttttg aacaccgttt accaagaggt
    23641 tgtgaatctg ccgtttaact ctgagtcaca aggaacaatt tcaaaaaaca gcttttcatg
    23701 tttgatgaat attgtgactt gtgaccgaga agttggcagg agaaaaccgt ttatacgaaa
    23761 aaagggactg tgacaaatcg tgtcacagtc ccttttttat agtgtttttt ataggtagta
    23821 ttttttagca actgttaagt catcatttaa ttcataaaca agtggttgac ctgttggaat
    23881 ttcaagatcc atgatgtctt cgtcagagat accttcgata tgtttagcta gggcgcgtaa
    23941 tgagttacca tgtgcagcta ctaaaacagt tttgttgtct agtaatgctg gagcaatttc
    24001 atcttgccag aatggcaaag cgcgttctaa tgtcactttc aagttttctc caccaggaat
    24061 atcgcgtttg tctaacattg cgtaacgacg gtcatttgct gcagaacctt cgtcagtttc
    24121 ttccattaat ggaggaagag tatcgtaaga acgacgccag atgtgcactt gttcgtcgcc
    24181 gtatttttca gctgtttctt ttttgttcaa accttgtaat ttaccatagt gacgttcgtt
    24241 taagcgccat gatttgattt gtggtaccca aagttgatct gaatattcta agatcaagtt
    24301 acatgttttg attgcacgag ttaaaacaga agtgtaagca acatcaaatt cgattccagc
    24361 ttctttgatt ttgcgtccgc cttcttttgc ttcttcgatt ccttctggtg ctagatttac
    24421 atcagcccaa ccagtaaatt ggttcaatgc attccattca ctaagaccgt gacgagaaaa
    24481 aactaatttt ggcatgttta gctctccttt taagataggt ttatatttct gaaattttca
    24541 gttctgtttt attgtacaat tttttggaag agatttccat cgaaaaatta gataagattc
    24601 tatgagaaaa caagtagaga atataaaaaa acgaacagtg cccacgcaac tgttcgtttt
    24661 taggtattag atgcataatt gctcgatttc tttttcaggt aaattgatat atttataaca
    24721 tgttccaact gaaacaccgc attttgaaga aataaattgg atcgtttctt ttttttcata
    24781 atagagacgt cggatttttt tgatcgtttt tgcatcgatt tttggtcttc cgccgatttt
    24841 tcctttttta cgagcttcat ctaatccaac taatgtacgt tctttgatca atgcgcactc
    24901 catgtttgct aggttttcca ttaattggaa atagattttt cccatttcgc gcgtatcgat
    24961 tccttcatca agactaatca agtgcagccc gcgatctttg aataattgcg tgagttctgt
    25021 taattgccgc gtagatttgc ctaatcgatg aagttggcaa atgactagtg catcgccttg
    25081 ttgcatgtca gcgatcactg actccaaatg aacagcagta gaatcatttt gttccacttc
    25141 atatgtctca tggaaaattt cttgacaacc aaagtcagat aattgatcaa cctgagttgt
    25201 taaatcaacg tcatttatgg ttgtgcgtgc ataaccaata attttcatgg tgattccttt
    25261 ctagcagtga ttttctaact ggatataaca gtaaaaatgt agcataaagt acgtcaaaaa
    25321 gatagttttt tcgcaaattt tccgctgtct ttttttattg gatgatcaag cagtttttct
    25381 ttatttacga cggattgcgt caagaatctc aacgcctttt tctgagccga caactacttt
    25441 agaaaccatg tttaggaaaa gcccatgttc tactacccca acaagttcat ctaaataggt
    25501 agctaagctt tctggctgtt cgatgacttc tagatgcaga tcaatgatgt aatgaccacc
    25561 atctgtggtt aagacttctc cgttttcgtc tgtacgcaag acgggttgga atcttttttc
    25621 ttcaaaaata tgaagtagct gttgggagcc ataagggatc acttcaactg ggagtgggaa
    25681 cttcccaagt ttatcgacta atttagaaga atcaacgatc caaatcgttt cttttgagta
    25741 cgtagcgact attttctcga aaagcaaagc agcgccgccg cctttgatcc cttgaaaatc
    25801 agcactgatt tcatcagcac cgtctattgt caaatcgact acggggactt catcaatact
    25861 ctttaacggt attcctaatt tttccgcttg ttcttttgtc gcatttgaag tagtaactcc
    25921 tgtgatacgt aaaccttctt cacgcatacg gcgaccaatc tcttctacca tataataggc
    25981 ggttgaaccg gtcccgagac ctacgatcat cccgtccttt acgaatttcg cggcttcaat
    26041 gccagctaat tgttttaaat tcatctttgc gtccaccttt ttttagtttg agcatcgtgc
    26101 tttcctcact attttagcgt gatttacggt aaaaagatac cattcttttt tattccaata
    26161 aagctttttc aaaaaaaatt gcatctcttg ttgacatgtt ttttcattcg tgatattcta
    26221 acaaaggtgc tttatctaca ggtttctgtg aagaaacgtg ctgactgttt atcaaagcat
    26281 atttgataag gatgcagaca ttgcatttat tttttatcac aaaaaaagaa ccacctggat
    26341 gtgtggaact agacgcaaat cgtgggaagg aggaaacaag gaatgcctac aattaaccaa
    26401 ttagtacgta aacctcgtaa atcaaaggtg gaaaaatcta attcacctgc tttgaacaaa
    26461 ggatataaca gctttaagaa aactcaaact aacgtaaact cacctcaaaa acgtggggta
    26521 gcaactcgtg tgggtacaat gacacctaaa aaaccgaact cagctttacg taaatatgca
    26581 cgtgttcgtt tgtcaaactt aattgaagtt actgcttata tcccagggat tggtcataac
    26641 ttacaagaac acagtgttgt attattacgc ggtggtcgtg taaaagactt accaggggta
    26701 cgttaccata tcgttcgcgg tgctttagat actgctggtg ttaacgaccg taaacaaagc
    26761 cgttctaaat acggaactaa aaaacctaaa gcttaatttt aattattaaa caaatgaaac
    26821 ttgcttctac tatatagaag taattactat gaacaatatt tcggaaggag gagttacgga
    26881 tgccacgtaa aggtcctgtt gcaaaacgtg atgttttacc agatcctatt tataactcaa
    26941 aattagtaac tcgcttgatc aaccgtgtaa tggttgacgg aaaacgcggg attgcagcta
    27001 acattatcta taatgccttt gacttgatca aagaatctac aggtaacgat ccattagaag
    27061 tgtttgaaca agcaatgaaa aacgttcaac ctgttcttga agttaaagca cgtcgtgttg
    27121 ggggttcaaa ctaccaagta ccagttgaag ttcgccctga acgccgcgtt actctaggtc
    27181 ttcgttgggt agtaaactac gctcgcttgc gcggtgaaca cacaatggaa gaacgcttag
    27241 caaaagaaat catggatgct gcaaacaaca caggtgcttc tgttaaaaaa cgtgaagaca
    27301 ctcataaaat ggctgatgcc aaccgtgcgt ttgctcacta tcgctggtaa gattctctct
    27361 gtttggtcgg cttttataag tcgacctcag atagtgtcct tatttttagc aactagatag
    27421 taaagagagg agaaacaagg agaatgacca gagaattttc tttacaaaac actcgtaata
    27481 tcggtatcat ggcccacatc gatgctggta aaacgactgc tactgaacgt atcttgtact
    27541 atactggtaa aatccataaa attggtgaaa ctcatgaagg agcttcacaa atggactgga
    27601 tggaacaaga acaagaacgt ggtattacga tcacttctgc tgctactaca gcacaatgga
    27661 agggtcaccg aatcaacatc atcgacacac caggacacgt agacttcaca gttgaagttg
    27721 aacgttcatt gcgtgtactt gatggtgctg taactgttct tgatgcacaa tcaggtgttg
    27781 aacctcaaac tgaaacagtt tggcgtcaag caacaactta tggtgtacct cgtatcgtat
    27841 ttgcaaacaa aatggacaag atcggtgctg acttcttata ttcagtatca actttacatg
    27901 accgtttgca agcaaacgct cacccaatcc aattaccaat tggtgcggaa gacgacttca
    27961 caggaattat cgaccttgta acaatgaagg ctgaaatgta tacaaacgat cttggaacag
    28021 agatcgaaga aacagaaatt cctgaagaat accgcgaatt agctgaagaa tggcgcgaaa
    28081 aattggttga agctgtagct gaaactgatg aagaacttac tttgaaatac ttagaaggtg
    28141 aagaaatcac tgaagctgag ttaaaagaag gtatccgccg cgcaacagtt aacgtagaat
    28201 tctacccagt actatgtggt tcagcattta agaacaaagg ggttcaatta ctattggacg
    28261 ctgttcttga ttacttgcca tcaccgttag atatcccagc tatcaaaggg attgacccta
    28321 aaactgatga agaagtagaa cgtcctgcag acgacagcgc tcctttctca gctttagcat
    28381 ttaaagttat gactgaccca ttcgtaggtc gtttaacttt cttccgtgtg tactctggtg
    28441 ttttacaatc aggttcatac gtacaaaacg caacaaaagg caaacgtgaa cgtgttggtc
    28501 gtatcctaca gatgcatgcc aactctcgtt cagaaatctc tgaagtttac gctggagata
    28561 tcgctgctgc tgttggtttg aaagacacaa ctactgggga cacactatgt gatgaaaaga
    28621 gtctagttat tcttgaatcc atggaattcc cagaaccagt tatccaagtc gctattgaac
    28681 ctaaatcaaa agctgaccaa gataaaatgg gtgtggcttt acaaaaacta tctgaagaag
    28741 atcctacttt ccgtgctgaa acaaacgtgg aaacaggcga aacaatcatc gctggtatgg
    28801 gtgaacttca cttagatatc atcgttgacc gtatgcgtcg tgaattcaaa gttgaagcta
    28861 acgtaggtgc gcctcaagta tcttaccgtg aaactttccg tgctggcact caagccgaag
    28921 gtaagtttgt acgtcagtct ggtggtaaag gtcaatacgg tcacgtatgg attgaattta
    28981 caccaaatga agaaggtgct ggtttcgaat ttgaaaatgc gatcgtcggt ggggttgttc
    29041 ctcgtgaata cattccagct gttgaaactg gtttgaaaga tgctatggaa aatggtgtct
    29101 tagctggcta tccattagta gatatcaaag ctaaacttta cgatggttca taccatgatg
    29161 tcgattcaaa tgaaactgcc ttccgtgttg ctgcttctat ggcattacgt gcagcagcta
    29221 agaaagctaa tccagtaatc cttgaaccaa tcatggctgt agaagttgtt atccctgaag
    29281 attatttagg tgatgttatg ggtcacgtaa ctgctcgtcg tggacgtgtt gaaggtatgg
    29341 aagctcgcgc tggcggccaa caagttgttc gtgcgatggt tccacttgct gaaatgtttg
    29401 gttatgctac aactcttcgt tctgctactc aaggtcgtgg tacatttaca atgacatttg
    29461 accactacga agatgtacca aaatcagtac aagaagaaat cattaagaaa aacggcggaa
    29521 aagctgaata atttcttaaa accggatagt cctgcaaaga gattatccaa agagactata
    29581 tttttaatat agtttcgtgt aaaataatta tcgatgatat gggcatgtgg tgtaccttgt
    29641 gtacatcata tacctatcag acaaataaaa aatatattgt atttttagga ggacatttta
    29701 aaatggcaaa agaaaaattt gaccgttcta aaccacacgt taacattggt actatcggac
    29761 acgttgacca tggtaaaact acattaacag ctgcaatcac aactgtacta tctaagaaaa
    29821 acggcggcca agctatggct tacgatcaaa tcgatggtgc tccagaagaa cgcgaacgcg
    29881 gaatcacaat ctcaacagct cacgttgagt atgaaacaga tacacgccac tacgctcacg
    29941 ttgactgccc aggacacgcg gattacgtta aaaacatgat cactggtgct gctcaaatgg
    30001 acggagctat cttggtagtt tctgctgctg acggcccaat gcctcaaact cgtgaacaca
    30061 tcctattgtc tcgtcaagtt ggtgttcctt acatcgttgt attcttgaac aaagtagaca
    30121 tggttgatga cgaagaatta ctagaattag ttgaaatgga agttcgtgac ctattaacag
    30181 aatacgaatt ccctggtgac gatgttcctg tagttgctgg atcagctttg aaagctctag
    30241 aaggcgacgc ttcatacgaa gaaaaaattc ttgaattaat ggctgcagtt gacgaataca
    30301 tcccaactcc agaacgtgac aacgacaaac cattcatgat gccagttgaa gacgtgttct
    30361 caattactgg acgtggtact gttgctacag gtcgtgttga acgtggacaa gttcgcgttg
    30421 gtgacgaagt tgaagttgtt ggtattgctg aagaaacttc aaaaacaaca gttactggtg
    30481 ttgaaatgtt ccgtaaattg ttagactacg ctgaagctgg agacaacatt ggtgctttac
    30541 tacgtggtgt tgcacgtgaa gacatccaac gtggacaagt tttagctaaa ccaggtacaa
    30601 tcacacctca tacaaaattc tctgcagaag tatacgtgtt gacaaaagaa gaaggtggac
    30661 gtcatactcc attcttcact aactaccgtc cacaattcta cttccgtaca actgacgtaa
    30721 caggtgttgt tgaattacca gaaggaactg aaatggttat gcctggcgac aacgtaacaa
    30781 tggaagttga attaatccac ccaatcgcta tcgaaaacgg tactaaattc tcaatccgtg
    30841 aaggcggacg tacagttggt gccggtgtcg ttacagaaat caaagcttaa ttcacttaag
    30901 ttttaaagta aagcaacatt tattcggacg taatcgctcg gaacgaaggt cttaggatct
    30961 ttgttccgag cttttttatt tattgcagga tcaggaaaaa agacaggagt attataaata
    31021 gaagaaacac gaaataaaag agtcagtatt ttctaaatga aactttatga aaacaaaatg
    31081 ttttttacaa gaaaaagcct tgctttgtga gtttatatat attataatga caaaggtgcg
    31141 tttactttag tgaattgcac agacgtaatc agaatgattt cgtcggctaa gaaacgagag
    31201 gttgcgacac gcccggacgc tttgccacga acgagcgtga cggaaatttt cgtggagcta
    31261 tgtctacttt tcaaaatagg cgaaggaggg aacaagatgg caaaacaaaa aattcgtatc
    31321 cgtttaaaag cgtatgaaca ccgtatttta gatcaatcag cggataaaat cgtggaaact
    31381 gcaaaaagaa ctggagctag cgtgtctggt ccaattccat taccaactga acgcagtctt
    31441 tacacaatca tccgtgcgac tcataaatac aaagattcac gcgaacaatt cgaaatgcgt
    31501 acacacaaac gtcttatcga cattgtgaac ccaacaccaa aaactgttga tgctttgatg
    31561 aagctagact taccatctgg tgtaaacatc gaaatcaaat tataaaacta aaacaaaaat
    31621 aatggaggtg tactcatgac caaaggaatc ttagggaaaa aagtgggaat gacacaaatc
    31681 ttcactgaat ctggtgaatt aattccagta actgttgtag aagctactcc aaacgtagta
    31741 ctacaagtga aaacaatgga aacggatggc tacgaagcta ttcaagttgg ttaccaagat
    31801 atgcgtgaag ttttatcaaa caaacctgcg aaaggtcatg ttgcaaaagc aaacacggct
    31861 cctaagcgct tcattcgtga attcaaaaat gttgagctag gagaatatga agtaggaaaa
    31921 gaaattacag tagatgtttt ccaagcagga gacattattg atgtaacagg tactacgaaa
    31981 ggtaaaggat tccaaggggt tatcaaacgc cacggacaaa gccgcggacc aatggctcac
    32041 ggttctcgtt accatcgtcg tcctgggtca atgggtccag ttgcacctaa ccgtgtattt
    32101 aaaaacaaac gtcttgctgg ccgtatgggt ggcaaccgcg taactatcca aaacttggaa
    32161 gttgtacgtg tcgatgcaga aagaaatgtt atcttgatca aaggtaatgt tcctggagct
    32221 aaaaaatctt taattactat caaatcagct gtgaaagcta aataattgta ggaagaaagg
    32281 aggaactaag gaatgccgaa tgtagcatta ttcaaacaag atggaagcca aaacggtgaa
    32341 atcacactta acgaagaaat cttcggaatt gaacctaatg aaagtgttgt ttacgatgca
    32401 atcgtaatgc aacgtgcgtc attaagacaa ggaacacacg cggttaaaaa ccgtagcgct
    32461 gttcgcggcg gcggacgtaa accatggcgt caaaaaggaa caggtcgtgc acgtcaagga
    32521 tctatccgct caccacaatg gcgtggaggt ggcgtagtct ttggaccaac accacgttct
    32581 tacagctaca aacttcctaa aaaagttcgt cgtttagcaa tgaaatctgt attgtcagaa
    32641 aaagttgctg aaaacaactt ggtagcaatc gaaggcttga acttcgacgc accaaaaaca
    32701 aaagaattca aacaagttct tgctaactta tctatcgact ctaaagtatt ggttgttcta
    32761 gaaaaaggta acgactttgc tgctttatca gcacgtaact taccaaacgt ttctgtagta
    32821 acttctgata atgtgagtgt gctagacgtt gtatcaaata caaaagtgtt agcaacacaa
    32881 actgctctta ctcaaattga ggaggtgctt gcataatgaa cttactagac gtaatcaaac
    32941 gcccagtgat cactgaaaaa tcaatgcttg ccatggatga caagaaatac acttttgaag
    33001 tggacactcg cgcaaacaaa actttagtaa aacaagcggt agaagctgct tttgacgtta
    33061 aagtcaaaaa cgtgaacatc gtgaacgtac gtgcgaaatt caaacgtatg ggcaaatatg
    33121 caggatatac aaaaaaacgt cgcaaagcga tcgttacatt gactgaagat tcaaaagaaa
    33181 tccaattatt cgaagctgct gaataagcac taatagataa ctcattaaat taggagggaa
    33241 aaacgtggcg attaaaaagt acaaacctac ctcaaatggt cgtcgtaaca tgacaggttc
    33301 tgattttgct gaaatcacaa catcaacacc tgaaaaatca ttgttacaac cattgaaaaa
    33361 ccatgctgga cgtaacaaca acggccgcat cactgtacgt catcaaggtg gcggacacaa
    33421 acgccaatat cgcgtgatcg acttcaaacg taacaaagat aacgttgttg caactgttca
    33481 aacgatcgaa tatgatccaa accgttcagc taacatcgcg ttagtacact atgaagatgg
    33541 agtgaaagct tacatcttag cacctaaagg actagaagtg ggtatgaaac ttgtttcagg
    33601 tccagaagca gatatcaaag tcggaaatgc tcttccatta gaaaacattc cagttggtac
    33661 tgttatccac aacattgaaa tgaaacctgg taaaggtgga caattgatcc gttctgctgg
    33721 tacaagtgct caagtacttg gtaaagaagg caaatacgta ttgatccgct tgaactctgg
    33781 tgaagttcgt atgatcttag ctgcatgccg tgctacaatc ggttctgtag gtaacgaaca
    33841 acacgaatta atcaacatcg gtaaagctgg acgctctcgt tggatgcgta aacgcccaac
    33901 tgtacgtggt agcgtaatga accctaatga tcacccacac ggtggtggtg aaggtaaagc
    33961 tcctatcgga cgtaaagcac cagtttctcc ttggggtcaa ccagctcttg gttacaaaac
    34021 tcgtaacaaa aaagctaaat cagacaaact tattgttcgt cgtcgcaaaa ataaataatt
    34081 actttgacca tgtgtcgcac attttgagag gaggttcacc atgggtcgta gtttaaagaa
    34141 aggacctttt gtcgatgagc atttaatgaa aaaagttgaa gctcaagcag gggttgaaaa
    34201 gaaaaaagtt atcaaaactt ggtcacgccg ttcaacagtt ttcccaagtt ttgttggata
    34261 taccatcgca gtttatgatg gacgtaaaca tgtaccagtt tacatccaag aagacatggt
    34321 aggacacaaa ttaggtgaat ttgcacctac aagaacttat cgtggacatg ctgctgacga
    34381 taagaaaacg aaacgctaat ttgaggggag gataaatcca aatggcagaa caaatcacat
    34441 cagctaaagc aactgcaaaa acagttcgca cttcacctcg aaaagcccgt ttagtaattg
    34501 accttatcag aggtaaaagt gtcgcagatg caatttcaat cttgaaattc acaccaaaca
    34561 aatctgctgg aatcattgaa aaagtattga tgtcagcagt tgcgaatgca gaaaataact
    34621 ttgacttaga tgttgaaaac ttggtagtaa gcgaagcttt tgttaacgaa ggacctacaa
    34681 tgaaacgttt ccgtcctcgt gcaaaaggat cagcttctcc aatcaacaaa cgtacaagtc
    34741 atatcacggt agtcgtatca gaaaaataag gagggataat cagtgggtca aaaagtacat
    34801 ccaattggaa tgcgtgtagg catcatccgc gactgggatg ctaaatggta tgctgaaaaa
    34861 gagtatgctg agttcttaca cgaagattta agaatccgta aatttatttc aactaaactt
    34921 gctgatgctg ctgtatcaac agttgaaatc gaacgcgcaa caggtcgcgt aaacatttca
    34981 attcacacag ctaaaccagg tatggttatt ggtaaaggcg gttctgaagt cgaaagccta
    35041 agaaaagaat taaacaaatt aactggtaaa agaatccata tcaatatcgt ggaaatcaaa
    35101 aaaccagatc tagatgcaaa attagtaggt gaaggaatcg cacgtcaatt agaaaaccgt
    35161 gttgctttcc gtcgcgctca aaaacaagcc atccaacgct caatgcgtgc tggcgctcaa
    35221 gggatcaaaa ctcaagtatc tggtcgtcta aacggagcag atatcgctcg ttcagaagga
    35281 tactcagaag gaactgttcc tcttcatact ttacgtgcgg acattgatta cgcatgggaa
    35341 gaagcagata caacatacgg aaaactagga gttaaagtgt ggatctatcg tggagaaatt
    35401 cttccaacta aaaaaaacac tgagaaagga gggaaataat catgttagta cctaaacgtg
    35461 taaaacaccg tcgtgaattc cgcggaaaaa tgcgcgggga agctaaaggc ggaaaagaag
    35521 tagcattcgg tgaatacggt ttgcaagctg ttgattcaca ttggatcaca aaccgccaaa
    35581 tcgaagctgc tcgtatcgca atgactcgtt acatgaaacg tggtgggaaa gtatggatta
    35641 aaattttccc tcacaaatct tatactgcca aagcaattgg ggtacgtatg ggttctggta
    35701 aaggggcacc tgaaggatgg gttgcaccag taaaacgtgg taaaatcatg tttgaaatcg
    35761 caggcgttcc tgaagaagta gctcgtgaag cgttacgtct agcttctcac aaattaccaa
    35821 tgaaaactaa gatcgtaaaa cgtgaggaaa tgggtggtga atcgaatgaa ggttaaagaa
    35881 atcagagaat taaccactgc cgaaatgctt gatcaagaaa aacaattaaa agaagaattg
    35941 tttaatctta gattccaatt agcaacaggt caattagaaa acactgcacg tattaaagaa
    36001 gtacgtaaat cgattgcacg catcaaaact gtgttgcgtg aacaagctaa gtaattatgg
    36061 aaggaggcca ttaagcgtat gactgaagga agaaatcaac gcaaagttta tcaaggtcgc
    36121 gtggtttcag acaaaatgga taaaacaatt accgttgctg tcgaaacaaa gaaaaaccac
    36181 cctatctatg gcaaacgcat gaactattct aagaaataca aagtgcatga tgaaaacaac
    36241 acagcaaaag ttggcgacat cgtgagaatt atggaaactc gtccattatc agctacaaaa
    36301 cgtttccgtt tgttggaagt agtcgaagaa gcagttatta tctaataaca cgagattttc
    36361 ctatatagat tgaatgcaaa atctgaaagg aggatacaca acgtgatcca agcagaaagt
    36421 cgtttaaaag tcgctgataa ctcaggcgca cgtgaaattt tgacgatcaa agtccttggt
    36481 ggatctggtc gtaaaactgc taacatcggt gatgtgatcg ttgctacggt taaacaagca
    36541 acgccaggtg gggttgtcaa aaaaggtgac gtcgttaaag ccgttatcgt tcgaacaaaa
    36601 tcaggagctc gtcgtacaga cggttcatat attaaatttg atgaaaatgc tgcagtgatt
    36661 attcgtgatg ataaaagccc tcgtggaaca cgtatctttg ggccagttgc acgtgaacta
    36721 cgtgaaaaca acttcatgaa gatcgtttca ctagcaccag aagttttata atcttgagac
    36781 acttatcaaa ggaggtgcga aacagttatg tttgttaaaa aaggcgataa agttaaagtt
    36841 atcactggaa aagacaaaaa caaagaaggc gttgtactag cagcgttccc gaaaaaagat
    36901 aaagttattg tcgaaggtgt aaatgtcgtt aaaaaacacc aaaaaccttc tcaagctgct
    36961 cctcaaggtg gaattgttga gatggaagcg ccaattcatg tttctaacgt gatggtggtt
    37021 gactctactg gtgtagctgg ccgagttggc tacaaagaag tagacggcaa aaaagtccgt
    37081 gtttctaaaa aaaccggtga agttttagat aaataaaagt ttaaggaagg agggtcttac
    37141 tgaatgaacc gcctaaaaga aaaatatctt aaagaagtaa ctccatcatt gatggaaaaa
    37201 tttaactata gctctgttat gcaaacacct aaagttgaaa aaatcgtcat caacatgggt
    37261 gttggtgatg cggtatcaaa cgctaaaaac ttagataaag cagttgaaga attaactttg
    37321 atcactggtc aaaaaccaat gattacaaaa gctaaaaaat caatcgctgg attccgttta
    37381 cgtgaaggaa tgccaattgg tgcaaaagtt acattgcgcg gagaaagaat gtacgaattc
    37441 ttagacaaat tggtttctgt ttcactacca cgtgtacgtg acttccatgg tgtaagtaaa
    37501 aaagcctttg acggtcgtgg aaactatact ttaggtatta aagaacaatt gatcttccca
    37561 gaagtagatt atgatttagt agataaagta cgtggtatgg acatagttat tgtaacaaca
    37621 gcaaactctg atgaagaatc tcgtgagttg ttgacacaat taggcatgcc attccaaaaa
    37681 taattaaagg aggcgaacta cgtggctaaa aaatcaatga ttgctaaaaa caaacgtcct
    37741 gcaaaatttt caacacaagc ttatactcgt tgcgaacgtt gcggacgtcc acattcagtt
    37801 tatcgtaagt tccatctttg ccgtatttgc ttccgcgaac ttgcctataa aggtcaaatt
    37861 cccggcgtga agaaagctag ctggtaaaaa gtaaattcgc ataagaagga ggtaaaagat
    37921 caatggtcat gacagatcca attgcagatt ttctaactcg tatccgtaac gcaaacatgg
    37981 ttaaacatga gtctttagaa gtacctgcat caaaaattaa acgtgacatc gctgaaatct
    38041 tgaaacgtga aggtttcgtt cgtgatgttg aatacatcga agatgacaaa caaggcgtga
    38101 tccgtgtttt cctaaaattt ggtaaaaacg gcgaacgtgt tatcacaaac ttaaaacgta
    38161 tctctaaacc aggtttgcgt gcttatgtca aagctgacga agtaccaaaa gtattgaacg
    38221 gtttaggtat cgcaatcatt tcgacttctg aaggtgttgt cactgataaa gaagctcgtg
    38281 aaaaaaatat cggcggcgaa gttatcgctt acgtatggta atcaagtaga atctacaagg
    38341 aggtgtcttt aagtgagccg tatcggaaat aaagtcgttg ttatccctga aggagtaaca
    38401 gttactcaag acggaaacaa cgttacagtt aaaggaccaa aaggtgaatt gactcgtact
    38461 ttttcagcag atatcaaaat gaatatcgaa ggaaacgaag taactttcac tcgtccaaac
    38521 gacagtaaag aaatgaaaac aattcatgga acaactcgtg cgaacttcaa caacatggtt
    38581 gtaggtgtca gcgaaggttt ccaaaaagcg ttagaactaa tcggggttgg gtaccgtgca
    38641 caattacaag gatcaaaact tgtattgaac gttggttact cacatccagt agaaatcaca
    38701 ccaccagctg gagtaactgt tgaagtacca gctaacacac aagtaattgt taaaggtgcg
    38761 aacaaagaag aagttggcga attagctgca aacattcgtg gaactcgtcc tccagaacct
    38821 tataaaggta aaggtattcg ttatgttggc gaatatgtac gccgtaaaga aggtaaaact
    38881 ggtaaataat cgtttagaaa aattgcatag aagccagcag caggaaaaag gcatttggct
    38941 gaagtttacc ttcaaaaaaa tacattttta cttgtgccgc agcttctatt tttcgaaacg
    39001 tatttactgc ttgaaatagt agtacaataa aaataaagag gtgacaattg tgattacaaa
    39061 accagataag aacaaaacac gtcaaaagag acatcgtcgt gtacgtaaca atatctctgg
    39121 tactgctgag cgcccacgct tgaacgtttt tcgttctaac aaaaacatct acgctcaaat
    39181 tattgatgac gtagcgggtg taacgctagc aagtgcctct accttggata aagaaatttc
    39241 aggtggaaca aaaacagaag cagcacaagc tgtcggtaaa ctagttgctg aacgtgcagc
    39301 tgaaaaaggc attaaagtag tagtctttga ccgtggtgga tacctttacc atggccgtgt
    39361 gcaagcttta gctgaagctg ctcgcgaaaa tggactagaa ttttagaaga aggaggaata
    39421 ccattcatgg tttatatcga tccaaaacat ttggaattag aagaccgcgt tgttgcaatt
    39481 aaccgtgtaa caaaagttgt taaaggtgga cgtcgtctac gttttgctgc tttagttgtt
    39541 gtcggtgaca aaaacggaca cgtagggttt ggtacaggta aagctcaaga agttcctgaa
    39601 gcaatccgta aagcaattga agatgcgaag aaaaacttag ttgaagtacc tatggttggt
    39661 tcaacaatcc cacacgaagt aatcggtgct tttagtggtg gtagaatcct aatgaaacct
    39721 gctgtagaag gttctggggt tgctgctggt ggaccagttc gtgccgtatt agaattagct
    39781 ggggtagcag atatcacttc taaatcttta ggctcaaata caccaatcaa cgttgttcgc
    39841 gcaacagttg aaggtttgaa acaattgaaa cgtgcagaag aagttgcaga acttcgcggc
    39901 aaatcagttg aagaattaat cggataagga ggacaacaat aatggctgaa ttaaaagtta
    39961 ctttaaaacg cagtattatc ggacgtcctc aaaaccaacg cgatacagtt aaagcgttag
    40021 gtcttacaaa gattaacagt tcagtggtta agccttctaa tgaagcaatt aaaggcatga
    40081 tcaatactgt ttctcaccta gtggacgttg aagaaattta atttagaaaa atcataacta
    40141 gaacattaag gaggtgccaa acctatgaaa cttcatgaat taaaacctgc tgaaggttca
    40201 cgccaagtac gtaaccgtgt tggtcgtggt acttcatctg gtaacggaaa aactgccgga
    40261 cgcggacaaa aaggacaaaa agctcgttca ggcggcggtg tgcgtttagg atttgaaggg
    40321 ggacaaacac cattgttccg tcgtttacca aaacgaggat tcacaaacgt gaaccgtaaa
    40381 gactatgcag tcattaatct tgatgtattg aaccgctttg aagacggagc tgaagtaact
    40441 cctgttgctt tagttgaagc tggaatcgtg aaaaacgaaa aagctggaat caaagtatta
    40501 gctaatgggg aactaaacaa gaaattaact gtgaaagcag caaaattctc taaagcagct
    40561 caagaagcaa ttgaagcggc tggtggatca atcgaggtga tctaatgttt agactattaa
    40621 aagatgcttt taaagtaaaa gacattagat cgaaaatcct atttactgta tttgtgttgt
    40681 ttgtatttcg cctaggtgca catattactg taccaggcgt aaatgctgct tcattgcaaa
    40741 acctttcttc tttgccattt ttcaacatgt tgaacttggt aagtggtagt gcgatgcaaa
    40801 acttttccat tttctcaatg ggggtttcac catacattac tgcttctatc gtcattcagc
    40861 ttcttcaaat ggacatcgtg cctcgatttg ttgaatggtc gaaacaaggg gaagttggtc
    40921 gtaaaaaact gaatcaagcg actcgatatc tgacattagt attggcattt atccaatctg
    40981 tcggtatcac tgctggattc aatcaatacg ctcaattagg tttcgtgaat aatcctaacg
    41041 tagcaactta cgtaacgatc ggtttgattc taacagccgg tacgatgttt gttacttggc
    41101 taggagaaca gatcacagaa aaaggtatcg gaaatggcgt atcaatgatc atttttgcag
    41161 gtatcatttc tagattgcct gaaagtatca gagaaattta tgaagattac tttataaata
    41221 ttgatccttc agacatatgg aaatctgcta tttttgtggc cattttacta gtagcggtct
    41281 tgttgatcgt gacattcgta acatttttcc aacaagctga acgcaagatc ccgatccaat
    41341 atacgaaacg cgtagctggc gcgcctacaa gcagctatct gccgttgaaa gtaaatgcag
    41401 ctggagttat tccagttatc tttgctagtt cgtttattgc tacgccaaat gctgttttac
    41461 aagcattgag cggtagattc ggttctgaaa actggtacaa agtgatgatg gagatcttta
    41521 actataatac agcacctggt gctacgattt atacgattct gatcgttgcc ttcactttct
    41581 tctacgcctt tgttcaagta aaccctgaga aattagcgga aaacttgcaa aagcaaggaa
    41641 gttatatacc aagcgtacgc cctggtaagg ggactgaaga gtatgtctca agattactga
    41701 tgcgtttaag tacagttggt gcagtcttcc ttggtttagt ggcattgctt ccaatcattg
    41761 ctcaaatggt ttgggatctt ccacaatcaa tcggattagg cggtacaagc ttactgatcg
    41821 ttatcggtgt agctttagaa actgcgaaac agttagaagg cttaatgctg aaacgtaaat
    41881 atgttggatt cattgagtaa gaaagcgtgt tgaggtttcc cctcaacaca cgttctcaca
    41941 catttattag gagggaaaac aatgaacctc attttaatgg ggctgccagg cgcaggtaaa
    42001 ggaacacaag ctgaaaaaat cattgatgct tatggtattc cgcacatctc aacaggagat
    42061 atgttccgcg cagcgatgca aaacgaaaca gctcttggcc tggaagcaaa gtcttatatg
    42121 gataaaggtg cattagttcc tgatgaagta acaaatggaa tcgtgaaaga gcgactagct
    42181 gaaccagata cagagaaagg cttcttatta gatggctttc cacgtacgct agatcaagca
    42241 aaagctctgg acgcaatgct aaaagatttg aataaaaaaa ttgatgctgt catcgatatc
    42301 catgttggtg aagaaatctt ggtcgaacgt ctagccggcc gatttatctg cagcaattgt
    42361 ggagcaactt accataaaat tttcaaccct acaaaagttg aagacacatg tgatcgttgc
    42421 ggtgggcatg aattctatca aagagaagat gataaacctg agacggttaa aaatcgtctg
    42481 gctatcaaca tcaaaaatag tgaaccaatc ttggcttatt ataaagaaca aggtttgcta
    42541 aacactattg atggtgctcg tgaaatcgat gctgtatttg cagatgtaca aaaaatcatt
    42601 gaaaaatagc acgattgccc gtagccgact tgcattatgc agttactctt gtcaagttca
    42661 ataaattatg atagaatatg tcagtccgac ttctagagaa tcctctagat gatccatttt
    42721 gagaattttg aggtgggaac tgcagtttcc gccattttat cgtgtgaaaa tgcgaaacaa
    42781 gtacaaggag gtactgtgcg tggcaaaaga agatatgatt gaagtcgaag gtacagtcgt
    42841 cgaaactttg ccgaatgcaa tgtttaaagt tgaactagaa aacggacacc aagttcttgc
    42901 tactgtttca ggtaaaattc gtatgcatta catccgtatc ttacctggtg acaaagtaac
    42961 ggttgaattg tctccatacg atcttaaccg tggccgtatt acttatcgct ttaaataatt
    43021 gtactccgta aaactacagg aggtattatc atgaaagtaa gaccatcagt aaaaccaatg
    43081 tgcgaacatt gtaaagtaat tcgtcgtaaa ggacgcgtta tggtgatttg cccagcaaat
    43141 ccaaaacata aacaacgtca aggataattg gaggtgctat aaatatggct cgtattgcag
    43201 gagtagatat tcctcgtgat aaacgtgtag tagtttcact tacttatatc tatggtattg
    43261 gtaacactac tgctaaaaag atcttagctg atgctggtgt atctgaagat gttcgcgttc
    43321 gtgaattaac gaatgaacaa acagatgcta tccgtgcaga agtagataaa ttaaaagttg
    43381 aaggggatct tcgtcgtgaa gtgaacttga acatcaaacg tttgatggaa atcggttctt
    43441 accgtggaat ccgtcatcgc cgtggattgc ctgttcgtgg acaaaacacg aaaaacaatg
    43501 cacgtactcg taaaggccca gctcgcgcta tcgctggcaa gaaaaaataa ttcttaagtg
    43561 aaggaggtta aaacttcatg gcagcaaaaa aagtgaatcg taaacgccgt gtgaagaaga
    43621 atatagaagc aggtattgca catatccatt ctacattcaa taacactatc atcatgatca
    43681 ctgatactca cggaaatgca ttagcgtggt catcagctgg ttcattaggt ttcaaaggaa
    43741 gcaaaaaatc aactcctttt gccgctcaaa tggcagcaga aactgctaca aaagcagcaa
    43801 tggaacacgg attaaagact gttgaagtaa cagttaaagg acctggttct ggacgtgaag
    43861 cagcaattcg ttcattacaa gctacaggtt tagaagtgac tgcaattcgt gacgtgactc
    43921 cagttcctca taatggtgcc cgccctccaa aacgccgtcg tgtttaatga gtgccgctca
    43981 ttttgagttt tatcagtaac gtcattgaac aagacacttt tcgttttgaa aggggtaaag
    44041 ataagaatga ttgagttcga aaaaccaaga atcgcaaaaa ttgatgaaga aaaagattat
    44101 ggcaagttca tcgttgaacc tcttgaaaga gggtatggga ctactttagg gaattcccta
    44161 cgtcgtattt tattatcttc tttgccaggt gctgccatca ctaatattca aatcgatggt
    44221 gtactgcatg aattctctac catcaaaggt gttagagaag acgtcactca aatcatcttg
    44281 aacattaaag gcttagcgct gaaaatgtat ggccaagaag aaaaaaccct tgaaatcgat
    44341 attaccggac ctgctacagt aactgctggc gatattatcg ttgatagtga tgtagaaatt
    44401 cttaacaaag atatgtacat ctgtagtgtc tctgaaggag ctacgttcca tgctcgctta
    44461 acagtgaaac ctggacgtgg ttatgttcaa gcagatgaaa ataaaaaaga agatatgcca
    44521 attggcgttc ttccagtcga ttcaatctac acacctgttc gtcgtgtgaa ctatcaagtt
    44581 gagaacacac gtgttggaca ccgtgaagat tttgataaat tgacgatgga gatttggaca
    44641 gatggttcaa ttgaaccgtt ggaagcaatg agtttgtctg cgaaaatcat gactgaacat
    44701 ttggatattt ttgtgaactt aactgatgaa gcaaaaaatg ctgaaatcat ggtcgaaaaa
    44761 gaagaaacac aaaaagaaaa aatgttggaa atgacgatcg aagagttaga cttgtctgtt
    44821 cgctcttaca attgtttgaa acgtgcagga atcaatactg tacaagaact tacaaataaa
    44881 tctgagcctg aaatgatcaa agtccgtaac ttaggacgta aatcattaga agaagttaaa
    44941 gcaaaattgc acgacttagg tttaggttta cgtaaagaag attaaaacct tttacgaagg
    45001 agggaaacca acgtgagtta tcgtaaacta ggacgcacat ctagccaacg taaagcgatg
    45061 ttgcgtgatt taacaactga tttattaatc aacgaacgta ttgtgactac tgaagctcgt
    45121 gcgaaagaag ttcgctcaac tgcagaaaaa atgatcactt taggtaaacg cggagatttg
    45181 catgcacgtc gtcaagctgc ggctttcgta cgtaatgaag ttgccagcgt gcgtgaagaa
    45241 aatgaggaca tcgtcattga atcagcttta caaaaactgt tcaacgatat tgcacctcgt
    45301 tacgcagaac gtcaaggcgg atacacacgc attttgaaga cagaaccaag acgcggtgat
    45361 ggtgcaccaa tggttgtctt agaacttgtc taaaatcaca ctaataacat cactttatag
    45421 atgattcttt tagagtgtta tgatgttgga agatattttt ccgagtctag ctcagcgctg
    45481 ctcctcaagc gaatatcgct tggggagtaa gcactcatca taaagtgttt ttttatacct
    45541 tcaattacct gtcatgacag atatgtttga aggcataaaa aaacatttta agcgagaaat
    45601 aattgaaaat tttgtctttt tttgatagtg ttagtaggaa ctttaggatg aagaaggagt
    45661 aatcatggag ccaattattg aattagaaaa gataaattat aaataccagc cagatgatct
    45721 tcgccccgca ttgaaagatg tctcctttac aattgataaa ggtgagtgga tcgctattat
    45781 tggacacaat ggttcaggaa aatcgacact tgccaaaacg atcaatggat tgcttctgcc
    45841 agaatcaggg atcgtgaaag tgggaaatca aatattggat gaagaaaata tttggacgat
    45901 ccgacagatg gttggaatga tttttcaaaa tccagacaat cagtttgttg gttcgacagt
    45961 agaagatgac gttgcttttg gtcttgaaaa tcaagggatt cccagagaag aaatgctagt
    46021 gcgtgtgaaa gatgcacttg agaaagttag aatggctgag tttgcttcac gcgagcctgc
    46081 tcgtttatca ggagggcaaa aacaacgtgt tgccattgcc ggcgtggttg ccttacgacc
    46141 tgatatcatt attttagatg aagcaaccag tatgctagat ccagaaggtc gtgaagaagt
    46201 gatctctacg atcaagaaga tcaaagaaga aagtcaattg accgttatat ctatcactca
    46261 tgatattgat gaagcagcta atgccaatcg gatactagtg atgagacaag gcgaacttgt
    46321 ccgtgaaggt acaccaaaag aaattttttc tgctggtcct gaactgatcg acttaggtct
    46381 agatctacca tttcctgaaa aactgaagag cgcactgaaa gaacgtgggg ttgatgtgcc
    46441 cagtgaatat atgacagaag aaaggatggt ggattggtta tggacatccg ttttgaacaa
    46501 gtagacttca cttatcagcc gaatacacca tttgaacaaa gagccttatt tgacatcaat
    46561 atgacgatca aagaaaacag ttatactgca ttagtagggc ataccggaag cgggaaatca
    46621 accttacttc agcatttgaa tgcgcttgtt aagccaacaa gcggtacagt ccatattgga
    46681 gaacgagata ttcagccgga tacggataat aagaatttaa agcccatccg aaaaaaagtt
    46741 ggcatcgtct ttcagtttcc agaagcacag ctattcgaag aaacggtagc aaaagacatt
    46801 gctttcggtc ctaaaaactt tggtgtcagc gaagaagaag cactagtcct agcaaaagaa
    46861 acattagaac aagttgggct ggatgaaagc tatttggaac gttcgccatt tgaactttca
    46921 ggagggcaaa tgcgtagagt agcaatcgct ggtgtgcttg ccatgaggcc ggaagtactt
    46981 gtattggatg aaccaacggc aggacttgat ccccagggca gaaaagaaat gatggagatg
    47041 ttttggcgtc ttcataagga acagcagatc actattgtat tagttacgca tttaatggat
    47101 gatgtagcca attttgcaga ttatgtctat gtgttggaaa agggacgaat cgttaatagc
    47161 ggggagccgc aagaggtctt tcaaaacatt gaatggctaa aagaaaaaca actaggcgta
    47221 cctactgcca ccgagtttgc agaagaatta atggctaaag ggatgaactt tgctaccttg
    47281 cctttgacag ctgaagaatt agcagacgct atcgtccatt atgctggagg aaatgcccat
    47341 gatgaataaa ttgatatttg gtcgttatat gcctggagat tccttgatcc atcgattgga
    47401 tccccgagct aaattattag ccagttttta ttttatcgga atcatttttt tagcaaataa
    47461 ttgggaaacg taccttttgt tggcattttt cacattactt tctgtcttct tgtcaaaagt
    47521 agatttgaaa ttttttatcc gaggcattcg cccattgatc tggctaatcc tcttcactgt
    47581 agctttgcag gtcttgttta caagtggcgg agaaacttat tggagttggg gtgttttcca
    47641 tatcactgaa tttggtgtga tgaacggcct ctttattttc tgtcgattcg tgttgatcat
    47701 ttttatgtcg actctactca cgcttactac accgcctttg gaattatcgg atgcgattga
    47761 gtacttgctt cgtccattga aagccgtacg gttcccagtc cacgaaattt cattgatgct
    47821 gtcgattgct cttcgttttg tgcctacatt gatggacgaa acagaaaaga tcatgaacgc
    47881 tcagcgcgct cgcggtgttg attttggtga aggaaaccta gttcagaaaa tgaaagcagt
    47941 cattcctttg ctgattcctt tatttgtcag cagcttcaac cgtgcggaag atcttgcgac
    48001 tgctatggaa gcaagaggct acaaaggtgg ggaaggaaga acgaaatatc gtgtattgca
    48061 ttggcataat agagacacga tggttatcat tgcttttgta ttattaactg tgctactgat
    48121 ctttttaaga ggataaacgt tcagtgggcc gctatctatt cttttcttgc tgatctgttt
    48181 gcaagaaagg atgcaagcag tgtatgacaa gaaatattag ggtctgtggc agactaaggt
    48241 cacaggcctc tttcttttat ctgaaaagaa agaacgaaaa aattttatag aaaaatcatt
    48301 attccagata accttttagg aacaaaggag agaaaaacat ggtacgttac aaagcaatca
    48361 ttgcgtacga cggaacaaat tttaatggat tccaaaaaca gccaaatggg cgtactgttc
    48421 aagaagaagt agaaaaaaca ttacaaaaaa tggcaaatgg aaaagaaatc acggtctttg
    48481 gttcgggaag gacagatgcc ggagtacatg cgatcgggca agtaatccat tttgattatc
    48541 cagaggagcg tccgttagaa cgaatgcgtt tcgcgctaga tacccagtca ccagaggata
    48601 ttgctgtaag acaagtagaa attgtttcag aagacttcca cgcaagatat ttggtcaaag
    48661 aaaagacgta tcaatttcga gtggatatag ggaaaccacg cagtcctttt cgacgccatt
    48721 atgcaagtta ttttccttac ccacttgatt tggaaaaaat tcagcgggcg cttcctgatt
    48781 tattagggac tcacgatttc acctcttttt gtgcatcagg aagctcgata gaggataaaa
    48841 tacgtacgat ctacgaggct aagatggagg taaatgaaac aaaagacgaa ttgttgttta
    48901 catttagggg aaatggcttc ttgtataaga tgatacggat cttagtagga acgcttttga
    48961 aaattggcaa tggccgattg cctgaagata gtatcccaga tattattgcg aaaaaagatc
    49021 gtaatgcagc aggacctaca gcacaccctg aaggacttta tttgtatgaa gtgaaatatg
    49081 aataagaggt tatgatagaa gtggtcagct tcaagaaata agaaggtatt tacgaaaatt
    49141 acttctcaaa attttgcaaa atttcggctt attttcgaag aagctacttc tggaacaccg
    49201 tttaagaaag aggtcgtgag cctgtcattt aactctgagt cacaaagagc aatttcaaaa
    49261 aacagcttct catctttgat gaatatcgtg acttgtggac gaggcgttga taagtgaatc
    49321 atctttttgt tacctatttt ttctgtaacc aaaaaacgat ggaacgttgt tttgattgcc
    49381 cttggtgcgg acagtctgaa caggttccat cgtttcttta ttttctacgt cttcttctgt
    49441 gtgtgtttcg tggttgttct tctattgctg gatcaatcgt gccataaaag gggcgattgc
    49501 ggtcaaggta ccagacataa agaataaaca atgcaataca taggacaaaa aggattgcac
    49561 ctatcgtaaa tccttcaggc aagtaactga aagtgacagt atgtttacct gctggaactt
    49621 ttatcatcaa gaatgcgtct ttgaaattgc tgaccttgac tggtttccca tcaattttag
    49681 ctttccatcc tttgtcataa ggaatagttg tcaccaacat ggaatcttca gcggtattta
    49741 cagtcccaga agcttttctg ccagtggctt ccatttcgac acctttttct ttgatctgat
    49801 tcattgcggc ttgataggct acaacatcta gaccaacgac tttaggctcc atgaaactta
    49861 tttctttcgt cccataaaaa cttgcagtaa atgtcactgt tgtctcttga ggataatagc
    49921 caagatcata gtattgacca gaaatgttga tttgcgtttt tcgcgatgta ccattgacag
    49981 tgatcgtcgc actagaactt tctaattgcg caaaattggt tgggaacaaa cttaagtaag
    50041 cttgtgtatt tgcaggcaca tggacttccc aagtgagctc ttttggctgg ttatctgctg
    50101 tttctgagta agtgacgcca gtcgttgtct ttgtgatcgt cgtattcaac tgattcttca
    50161 aagtaggtgt atagaatgta aaatactgtt catttagacc agacaatgta ttgatcaagt
    50221 ttgtctgact ggtcaaatta tctgtcactg gctgctcaac atcattaacg gacaacggtg
    50281 cagtaaatgc tagtggcaat gcattgacat tctgatacag tgtatagtcg ccgctttgac
    50341 cagcttggcg gaagccatat ttactgaacg tgctttcgtt tttagaaatg ttatatttga
    50401 ttcctgtaaa cccatccatc agcaaagtat tattgttata acggatattc aaatttgtcc
    50461 ctcttgaacg gaaacctaat ttatccaggt aagaggaaga attacgattt cttatagaag
    50521 aaaacaaact gataccactg taaccatagt tgatcgaatc gtttgcagat actggatcca
    50581 gattttccaa gcggtaaaac gagtcggaat ctttttttgt ttcatttaca agagctttca
    50641 agtcaggata aggatcagta tataaactgc gggaagcata attccaatca tcaagaatgc
    50701 catggatcat actgttcgta ttgaccagtg cttcagcaga catgatcaat aataacaata
    50761 tactcagatg attgaatgaa agttttttat gctgataaaa tcccacagca aaagcataaa
    50821 tccctaagaa aaccactgtc aatacaaagg aagtcatcgt cacataggtg tagcttcctt
    50881 caggtttgat gccccatgcc accgcaaaga gagcagctac gaccaaaatg attcctaaca
    50941 acagcccacc atcgtcttgt tctatctttt cccatccttt tcctgcaagc aggagcacta
    51001 agaaagagaa cagataggca taacggaata aaaacatatt cggtgcatgc ataccatgcc
    51061 agaacaaatt caatggaaca agataaaaac ttgcgataag tatggcgaaa agactgccgt
    51121 acaaaaattt atttttcaac ggtactttcc gactggcaaa ataaaacaga cagaggacta
    51181 atggtagcag acctgcgtaa acgaacggaa tcgatccgta ttttgtcgtg tcataaacac
    51241 caatcatatt tttcatgatt agatccagat aagaagtcgc ttctgttttc aacgtagtga
    51301 tctgactcag tgcctcgcca tttgaccgaa gatccagcac tgctggcaat acaatgatca
    51361 ttgaagcgcc gcctgccagt aaagaagtga taccataagg aacaatcgtt tttttatatt
    51421 ctgaccagtt tgtcaataga cgggcgataa aatataagaa agaaaatacg ccaatcataa
    51481 aacccatata aaaactggat aaaaacaaga gtaaataact gataaataaa agtttcggcc
    51541 gtttaagatc cataacacga tgaatcccca aaatcactaa tggaagataa acaaatgcat
    51601 ccagccacat gatgatctcc gaatgtgcag tgataaatga gatcaatgcg taactgacgc
    51661 taagtgcaac atgcccccag cgaggaatct tgaacgtacg tgcaagaaac caaaaagcca
    51721 atccggcaca gccgacctta agcaaagtca gaacatacag tgcatctggc atcatctggt
    51781 ttggaaaaaa gaacaccagt ggcgtgaaga tcccgcctag atagtaagaa attaaagata
    51841 aataattcaa tcctagagaa gcattccatg tataaaaaat actttgcttt ccaagaagaa
    51901 catttcggaa gctggcatga aaattagaaa actgtgaaaa ggcatcactt gccaaaatac
    51961 ttctgctgct tccaggatag attccgatac ttagataaac aagtgctaaa atcaaaaacg
    52021 gaatcaggaa actagctgct gcgtagcgtc cgttttctct aaagaatgac tttaaatttt
    52081 ttttcatatt tattttccac caaatttcta tgaagttgta ttcagtttat catatccttg
    52141 tttttctctg cttaaaaaga gtctagtttt cacaaaaaaa taaaatttag aataaataac
    52201 ttcataattt gttcgtataa caaaaataaa cgcaaaaaaa gggtaggaat caaaagtccc
    52261 acccttaaaa atttttatta aataaaaaac cgttcactat ctgaacggtc caagtatcat
    52321 tcaattaaac gcgttcaact tttcctgatt tcaaagcacg agttgacacc caaacttttt
    52381 taggttttcc gtcgaccata acgcgaactt tttgtaagtt aggtttaaca gtacgttttg
    52441 ttgagttcat agcgtgtgaa cggttgtttc cactttttgt cttgcgacct gtaaagtaac
    52501 aaacttttgc catggtaatt tcctcctttg ccttgatttg gagcaataga ataattcagc
    52561 tcataccaga ctaacatatc atatagaatg ggaatttgca agtacatttt catagataca
    52621 attgacaatt gagaagaata attgtaaatt aaacaaggtt atacctgaaa ttttgcaagg
    52681 tttttgagta ctttgggtaa ctgcctttta tttcaagttg cgctatgata aaatatgata
    52741 gaagaaaaat gggagttttt tacccgttaa ggaggctcat tcatggctgt aaaaattcaa
    52801 acgtcagctg gaaccattga aattaccaat gatgtaattg caacagttgt tggtggcgcc
    52861 gcgacagatg tgttcggtgt tgtaggaatg gccagcaaaa atcaaattaa agataatatt
    52921 actgagattt tgcgtaaaga aaattactca agaggagtag ttgtccgcca agaagataat
    52981 ggtattgctg tagatgttta tacgattatc agctatggta cgaaaatctc tgaagtttcc
    53041 cgcaacgtcc aagaaaaagt gaaatacaat ctagaggcat tattaggcgt aactgccaat
    53101 tcagttaatg tctttgtaca aggtgttcgt gtactgccag actaggcagg agactggctg
    53161 gtaactcagc agtcgatctg aaggaggatt atctaggtga gtgtaacaga aataagtgca
    53221 agccagttcc aggaaatggt ccaggcaggt gcgaatcgtc tgcaagtaaa tgcagaatac
    53281 gtcaattcat taaatgtatt ccctgtgcca gatggcgata cgggaacaaa tatgaatcta
    53341 tctatgacta gtggagctaa ggcagtagta gattcaacct cagaaaaagt aggagaatta
    53401 gcagcaatgc tttcaaaagg attgctgatg ggtgcgcgcg gaaattccgg cgttattttg
    53461 tctcaattgt ttcgtggatt ttctaagcaa attccagatg ttgtctcatt aaatgcttct
    53521 gatcttgctg cagccttcac tcatggagtt gaaactgcgt ataaagcagt aatgaaacca
    53581 gtagaaggaa cgattttgac agtagctaga gaagcagcga aagctggaga aaagaaagca
    53641 aaaagtactg atgatgtcgt tgaagtaatg acagctgttg taaaaggagc aaagcgtgct
    53701 ttagctaaaa ctccagactt gcttccagtc ctaaaagaag tcggtgtcgt agacagtgga
    53761 gggcaaggac tgctgtttgt ttatgagggc tttttaagtg cattgaatgg agaattccaa
    53821 gcggatgaaa catatgaacc ttctccagca gaaatggatg atatggtcaa tgcagaacat
    53881 catcgcagtg tccaaggtca aatggcgaca gaagatatcc attttggtta ctgtacagaa
    53941 atcatggtaa aaatcggaga aggacctaca gtcgatagta cattcgatta cgaagaattc
    54001 cgtaactatt tagatggtat aggagattct ttacttgtgg tcaatgatga cgagatcatc
    54061 aaagtccacg tgcatacaga aaacccagga gaagtcttga attatggaca aaaattcggt
    54121 tcattagtca aaatcaaagt cgacaacatg cgcttgcagc atgagacgat tttggaacac
    54181 gatgaagaag tgaacgagtt tgcgaaaaaa gaaataccac gtatcccgta tggtatcgta
    54241 gcgatcgctg ctggtaaagg cgtccaagag ctgtttgaaa gtttaggcgc taattatgtt
    54301 atcagtggtg gacaaacaat gaatccaagt acggaagaca ttgttaaagc gattcaagaa
    54361 gtaaacgcag ataaagtgat tgtcttgcca aacaataaga acatcttcat ggcagctgat
    54421 caagcagcag aagtagcaga tatcccagta gcagttgttc cttcaagaac gatttcccaa
    54481 ggattgacag ctatgctttc attcaatgat caagcttctt tagaagaaaa caaagaaacg
    54541 atgacagaaa tgttgtctag cgttgtgagc ggtcaagtca ctcacgcgat cagagatacc
    54601 tcaattgacg gtgtagagat caaagaagga gactttttgg gcatgatcga tggaaaaatc
    54661 ctgatctcta acccagatat cacagcaaca gcgttaagta cactagaaca aatgatcaat
    54721 gaggacacag agatcgtaac gatcttggca ggtgatgaag gaacagttga acaagcaaat
    54781 gaatttgctg agaaactaac agaaaaacat cctgatttgg aaatcgagat ccaccaagga
    54841 gatcagcctg tctatcctta tctattttct gctgaataat ttctgtgtca gaatatcttt
    54901 ctaacacagt attcattcat aaaaggcctt cggttagtga tcaggtatga tcgctgacca
    54961 agtccttttt ttgttaaagg agggaggaaa aatttatgcg ttctttagat gatccaatca
    55021 ctatgctggc aggagtcgga gaaaaaagag cagctagtct tgccagcttg ggcatccaaa
    55081 cgattgaaga cttactgact tattatcctt ttcgctacga agatattcag gaaagaaatc
    55141 taaacgagat ccaggatcaa gaaaaggtta ctttgaaagg aatcgtagtc tcgcctcctg
    55201 tgatgaatcg atttggttat aaaaaaagcc gattgcaatt tcgtatgatg caagaacatg
    55261 atgtgttcaa cgtttctttt tttaatcaac cttatctaaa agaaaaagtc attttatcgg
    55321 aggaaatcgc cgtatacggt aaatgggatg caaagcgtaa agccttgaac gggatgaaga
    55381 ttttaggttc tcaaacaatg gatgactttt cgccgatata tcacgtcaac aaatcaattc
    55441 gtcaaacgac attagttgat ctaatcaaaa aagggtttca agaatacggc acattgattg
    55501 aagaaaatct gccaaacaac cttgtggaga aatatcgact gcttgaccga gcttctgctg
    55561 taagaagtat gcattttcca aaagaccacg aagaaaacca tcaagccaaa cgacgtgtcg
    55621 tttttgaaga gttcttttta tttcaaatgc gtcttcaagg attgaaaaaa gcagaaaaag
    55681 cagaaacgaa tggtttggag atcctttatg atgtccaaaa attgaaacaa tttactcaaa
    55741 agctaccttt tgaactgaca aatgcacaaa aacgtgtaac gaatgaaatc tgtcgggatc
    55801 ttcgcagtcc gcagcatatg cagcgacttt tgcagggaga tgtaggtagc ggaaaaacag
    55861 tggttgccgc gattgcgtta tacgctaccg ttacagcagg tttccaaggt gcgttgatgg
    55921 tgcctacaga aatcttggca caacaacata tggaaagctt gcaacagctg tttgatccca
    55981 atgaagtaac catcgcattg attacggggt caacgaaagc aaaagaacga cgtgagctcc
    56041 ttgaaaaact agagcaaggg gaaattaacg tcgtgatcgg cacacatgca ttgatccaag
    56101 atggagtcga atttcagaat ttaggtctag ttattacgga tgagcagcac cgttttggcg
    56161 tgaaccagcg gaaagtactt cgtgaaaaag gctggcgacc agatgtctta tttatgacag
    56221 cgactccgat tcctagaaca ttagctatta cagcatatgg tgagatggat gtatctatca
    56281 ttgatgaact tccagctgga cgtatcccga tcgaaacacg ctgggtaaga acacctcaat
    56341 tggacagtgt actggattgg acgaaaaaag aactggcaag aggacatcaa atgtatgtca
    56401 tctgtccgct gattgaagaa tcagaagcat tagatgtaaa aaatgcagtg gaaatctatg
    56461 aaaaacttcg tgaactattt gcaccaaaat atgaagttgg cttgttacat ggtaaaatga
    56521 agaatcagga aaaagaagta atcatggaag ccttcaaaga caaccagatg cagatattag
    56581 tttcgactac tgtaattgaa gtaggggtaa acgtgccgaa cgcgaccgtc atgctgatca
    56641 tggatgcgga tcgatttgga ttggcacagc tgcaccaact tcgtggacgt gttggacgcg
    56701 gaagtgatgc ttcctactgt atcttagttg ctaatcctaa aaatgaacta ggtgtggaac
    56761 ggatgaaaat catgacagag accaccaatg gttttgtact aagcgaaaaa gatctggaat
    56821 tacgtggtcc tggtgaagta tttggcttcc gtcaatctgg tctgcctcaa tttgcgattg
    56881 ctgacttggt gacggatgga aatgttttgg aggtagccag agaagaggct gcagcccttt
    56941 ggcaaataaa ggattggcag atgcttcctg agtatcagcc tttagcgcaa agcctccaaa
    57001 tagaaaatca ggataatcgt tatttcgatt agcagactgt gctatacttt atactgatga
    57061 cggaaaaagg agtggaaaca atgaaaattg ccgtagatgc aatgggcgga gatcacgcac
    57121 ctcaagcaat tgtagaaggc gtggctttag cgcagcaaga ttttccagaa gtagaattct
    57181 tactttacgg aaaagaagca gaaatcaaga aatacttaac aaatgaaaaa aacatcacga
    57241 ttatccatac agacgaaaaa atcaatagtg atgatgagcc agtaaaagca attcgacgaa
    57301 aaaagactgc atcaatggtc cttgctgcac aagcagtgaa aaacggtgaa gcagatgctg
    57361 tattttcagc agggaataca ggcgcgctac tagcagcagg gttgttcatc gtaggacgta
    57421 tcaaaaatat cgaacgccct ggcttgatgt ctaccttgcc agtagtaggg aaagaaggtg
    57481 ccggtttcga tatgctggat cttggcgcta acgcagaaaa caaaccagaa catttgttgc
    57541 aatacggtat tttaggttct ttttatgcac gtaaagtccg aggtatcgga cgtcctcgtg
    57601 tcgcactctt aaacaacgga acagaagcaa caaaaggtag tgaagtgacg aaaaaagcct
    57661 atgaactcct tcagaatgaa acaagcttga attttattgg aaacgtagaa gctcgtgagc
    57721 tactcaatgg tgtggctgat gtagtggtca cagatggatt tacaggcaac gctgttttga
    57781 aatctattga aggaacagca atgaatatga tgaatctatt aaaatctgct attctaaatg
    57841 aagggatcaa aggaaaaatg ggcgctatgc ttttaaaaga cggcttacgt tctttgaaag
    57901 cagaaatgga ttattccaaa catggcggtg ccgttttgtt tggcttgaaa gcaccagtca
    57961 ttaaaactca tggagcaaca ggccccgatg ctgttcgcta tacgatccgc caaatccaca
    58021 caatgcttga gacagatgta gttggacaat tagtcgaaca atttgaaaaa agcgaagaat
    58081 agtatttgga tatctgaagc gatccgttcc attgaaaaaa aggtggacat tcctgaaaaa
    58141 tgccagtaaa attagccatg gtgtaggtaa ttgacaagtg tatgattctt ggaggtgaaa
    58201 aagttgactc gcgaagaagt atttaacaaa gtagcgaaaa ttatttcaaa tcattttgag
    58261 atagataccg ataaagtgac ggatgaattg aacatcaaag atgatttaaa agctgattca
    58321 attagtatta tggaattcgt tttagagctt gaagatgaat tcggtacgga aatttcagat
    58381 gaagacgctg aacaaattga aacagtcggt ggcgcagtag attatatcag cagccatctt
    58441 aaataatttc aaataaaagc aaagaaggat aaaaagctgc atgcagcttt ttatccttct
    58501 ttttttaatc tttttgacac gtaaaagtca tttttctgat aattctggcg ataaacagaa
    58561 attatgttgc aaagtaaagc ggttttctat ataattatta ataattcatt acacaaagat
    58621 aagaataacc tttgttataa aacgatttat tctgtgtaat gtcctttttt attaagaaaa
    58681 acttccattt aggaagcaga gaggggtatt cgattgtccg ataaccaatt attagaagtt
    58741 cagaacctac atacaggatt ccgactcaaa gatgactatt acgatgcagt agatgacgtt
    58801 tcatttactt tagacaaaaa cgagatttta gcaatcgttg gggaatctgg gtgcgggaaa
    58861 agtaccttgg ctactacgat tatgggatta cttgatccta ataacacaaa aattacaggt
    58921 gagattttat ataacgatct gaatttaatc gacctgaacg aaacgctcta taataaaatc
    58981 cgcggaaatg atatcggaat gattttccaa gatccgttgt ctgcattaaa tcctctcatg
    59041 agaatcgaag accagatcaa agaaggactt tcctatcaca caaagatgaa tgcagaacag
    59101 cgtcaagctc gtgcactaga attgctagaa caagtaggga ttcctaatcc tgcgagagtt
    59161 ggacgccaat atccccatga actatcaggc ggtatgagac aacgtgtcat catcgcaatc
    59221 gcaatcgcct gcaaacctcc aatcgtcatt gcagatgaac cgacgacagc cctagatgta
    59281 accatccaag cacagattct tgatttactg aaagaccttc aggaagaaac aaaaacaggg
    59341 atcatcttga tcactcatga cttaggagtc gtagcagaaa tggctgatcg agtggcagtg
    59401 atgtatggcg gacagttcgt tgaagtagca ggtgtaaaag aactgtttga tcatcctaag
    59461 catccttata cacgctcttt actgaactct ataccacaag aaggaaatca tgatgcagaa
    59521 ctccatgtga tcgaaggtgt cgttccttct ctgaaaaaca tgcctcgtaa aggttgtcgg
    59581 tttgcaccac gtattccatg gataccagca agcgatcatg aagaagagcc tatccttcat
    59641 gaagtcgcac cgaaccactt ggtacgttgc tcctgctata agcatttcca ctttagagac
    59701 gaaaaaggag aggtgtagat gacagaatta atcacaatca aagacctgaa agttcattac
    59761 cctattcgca gtggattctt caatcgagta acggatcatg tatatgctgt agatggtgtg
    59821 gattttatta tcgaaaaagg aaagacatat ggtttagtgg gagagtctgg ctctggaaaa
    59881 tctactacgg gaaaagccgt tgtgggatta gaaaaggtaa cagccggaca aatcatgtat
    59941 gaaggaaaag atgtaacgaa gcgaagcaac cgcaaaaaaa tgggttataa caaggatgtg
    60001 cagatgatct tccaagattc aatgtccagt ttgaatccta aaaagcgagt gttggatatc
    60061 attgctgaac ccatccgaaa ttttgaacgg ttaagcgatc aagaagaaaa gaaaaaggta
    60121 aaaggactgc tagatatcgt tgggatgccg gaagatgcgc tatataaata tcctcatgaa
    60181 ttttcaggcg gacaacgtca acgtctagga gtagcccgtg ctgttgctac caatccaaaa
    60241 ctgatcgtag ccgatgaacc tgtatctgct ctggatctat ctgttcaagc gcaagtattg
    60301 aatttcatga aacgaatcca gcaagaatac ggcctcagct atttattcat ttcacatgat
    60361 ttaggtgtag taaaacacat gtgcgacaat atcgcaatta tgtacaaagg tcgatttgta
    60421 gaaatcggta caagagaaga catttataat gacccgcgac atatctatac gaaacgtctg
    60481 ctttcagcta ttccgcaaat cgatgtagaa aatagggaaa gccacaaaga aaatcgacga
    60541 agagtagaac aagagtatat ccaaaaccaa aaagaatact atgatgcgac tggtcgagtc
    60601 tacgatttgc gtacgatcac aactacacac aaagtggcgt tgaaagatgg aggtgcaagc
    60661 taatgtggaa aacgattctg cgacgtgttt tattgatgat cccgcaagtt attattttaa
    60721 gtgttctgat cttcttatta gcccaagcga tgccggggga tccatttacc ggtttgatca
    60781 atccaaatca ggatccggca gtgatcgaac agatgcgaca agcagctggc ttgaatgacc
    60841 catggtatgt ccagtacttc cgttggatcg gaaatgcttt gcaaggcgat tttggtcaaa
    60901 gtttcttata caaacgctct gttgctgact tgatcggcga acggatcatc aacactttat
    60961 atctatcgat cttgacagtt gtccttactt atttgattgc tgtgcctctg gggatgcttg
    61021 ctggccggta tcaaaattca atcttggata agattgtcgt aatctacaac ttctttagtt
    61081 ttgctgttcc tttatttatc ttcggcttga tcatgctatt tgtttttggt tatcgtttag
    61141 gctggttccc aacaagtgga tcacaaacac taggtttgca aggtggattt atcactcaat
    61201 ggatagaccg tttgaaatat attctgttac catcaattac ccaagcattt ttggcgactg
    61261 ccgtcacaat ccaatatctg cgaagtgaag taatcgattc gaaatcgctc gattttgtca
    61321 gaacagcccg ttccaaaggg gtaccgacga ataaagtatt cagtcgacat attttccgaa
    61381 atgcagcgtt gccgatggct tctcaaatgg ggtatgaaat cacttcgttg attgctggtt
    61441 ctgtagttat cgaaaagatt tttggttatc cgggagtcgg aaaactattt attgattcca
    61501 tcggccaacg ggactatacc gtgatcacat ccttagtatt gattttagga atcgcgacgc
    61561 tagtggggac attactttca gatatcatca tgagtatcgt tgacccgcgt attcggattc
    61621 aataaaacca atcagaaagg agaatggatc gatgagtgaa aaaaacaata cagcagttca
    61681 agaaagtatt cctcctatgg ggatacgaat gattgctcga gaatttaaaa aagataaaat
    61741 tgctattttt tcattaggtt tgctagtggt tttattatta gtgatcttta tcggtgcgat
    61801 gctgaccgat caagacaaag tgatgtatgt tagtatcttc gacaaatacg ctgcaccagg
    61861 aacagtttcg acacaaggac aaaagttcat tctgggagca gatgaaggcg gacgtgatgt
    61921 attaggacaa ttgatcatag gtgctagaaa ctctgttacg atcggatttg cgatcacgat
    61981 ccttacttcg atcatcgggg ttggattagg gatcatctct ggctactacg gagggatcat
    62041 tgacaacatc ttgatgcgga tcgttgattt tatcatgatt ctaccgatca tgctgatcat
    62101 tatcgtcttc gtatcgatta tttccagata caatgtctgg tcctttatat tcatcatgag
    62161 tgcattttat tgggtagcta aagcccgatt attccgaagc aagacattat ctgaagcaag
    62221 aagagattat gtcagtgcct caaaaacaat gggaaccaat gattttaaaa tcatgttccg
    62281 cgaaatcatg ccgaatctaa gttcattaat cattactaat ttgacgatga actttgcggc
    62341 aaacatcggt atcgagacaa ctttgacttt cttaggtttc ggcttgccgg caaatgtacc
    62401 tagtttaggt acattgatcg gttatgcaag taacggagaa gtcttagtga atcggcaatg
    62461 gatctggtta cccgcatcaa tccttatttt agttttgatg ttgagtatca attatgttgg
    62521 acaagcattc aagcgttctg cagatgcgcg ccaacggtta ggctagtata gaagcgaagg
    62581 gggaaatagg aatgaagaaa acgatttttg gatttgcagc gctgatgtct gctgtcgttc
    62641 ttgcagcatg cgggggcgga ggtacagata cctctggaaa caactctgga ggcaacggca
    62701 ataacaaaga ggaattaact tttccaattg caacaaaaaa taatgatgat gcaatcgaag
    62761 acggcgaatt agaagctgcg gtcgcaacgg acagtcagtt taaaggattg ttccagtggg
    62821 agttttacca agattcatat gatgcagctt ttatggctcc ttctcatgaa ccgctatttg
    62881 caaacgatga cgatttcaga atcacagacg acggagctgc aacgcttgat ctagatgaag
    62941 atacgaaaaa agcgacaatc aaagtaaaag acaacgtcaa atggtcagat ggtgaagatg
    63001 taacggcgga cgatgtgatt ttcccttacg aaatcattgg gaataaagat tacactggta
    63061 tccgttatga cgacactttc cgtaacatcg taggtatgga agaatatcat gatggaagtg
    63121 ccgacacaat ctccggaatc aaaaaaatag acgacaaaac agttgaaatc gaatataaag
    63181 aagtaaaccc aagcatgctt caatcgggtg gtggtatctg gtcatatgca gcaccaaaac
    63241 atacgttaga aggtgtagca atcaaagata tggaatcaag cgatcaagta cgtaaaaatc
    63301 cagtgacttt tggcccttat tacatgagta atatcgttgc aggagaatca gtagaatact
    63361 tgccaaacga gcactactgg aatggtacgc ctaaattgaa aaagatcacg atgaaatctg
    63421 taccgatggc aagtgcaact gaagcattga actctaaatt atatgatatg attttccaaa
    63481 tgccaacaga tacttacgat acctataaag aaattgctgg ctatacaaac ttaggccgtg
    63541 aacaaacttc ttatacttat ctaggattca agttaggtaa atggaatgca gatacaaaga
    63601 gtgtggaata caatccagat tcaaaaatgg ctgataaatc attacgtcaa gcaatgggct
    63661 atgcattaga taataatgca gtaggtgaac gtttctatgc aggtttgaga agcaatgcga
    63721 cttcattgat tcctccagta ttccagtcat accatgatga tgagatgaaa ggattcacac
    63781 aagatctaga caaagcgaaa aaactattag atgatgctgg ctacaaagat gtggatgacg
    63841 atggtttaag agaagacaaa gatggaaata aactaacaat caactttgct tcgatgtccg
    63901 gtggggaaac agcgcaacca ttggcagatt actatatcca acaatggaaa gaaattggtt
    63961 tggatgtcca atacacaaca ggtcgtctga tcgaattcaa ttctttctat gataaattag
    64021 aaaacgatga tcctgaaatt gatgtttacc aagctgcttg gaatacagga actgatccaa
    64081 atccaagtgg actatgggga ccaaatgctg cgttcaacta cactcgttat gaatcagaag
    64141 aaaatacaaa attgatcaac gatatcagtt caagcaaagc atttgatgaa gagtaccaaa
    64201 aagaagcata tcaaaaatgg caacaatatg catttgaaga agcatttgcg attccaacgt
    64261 tgttccgtaa tgaagtattg ccagtcaacg accgtgtcaa agactgggat tggtcttatg
    64321 atgtaccaaa cccatgggca aatgtttctg ttacagcaga ttcacgttca taatcactaa
    64381 aaatgaccga aaaagggcga tgtccttttt cggttttttg atatttttct gtttatttct
    64441 ctaatattca ggtcaaggcg ctgatttcat gacttctcca ataaacggtg ttcaaagtcc
    64501 agtttctcgg tattaagtca aatttatgca aaaatatgga aagccatttt cacaaaattt
    64561 tttcttcata ctcgaaacta aacgaatttt tcacaacttc tcgtcaatca ctaggattat
    64621 taaagcttta cttacctttt gaccgaacca tttcaaattt gacaaaaaac cagtataata
    64681 aggaagtatc gaaaaaatat ccataaaagg aatgtggaaa atggacaacc agctgacaaa
    64741 agaactaaaa gaaaactata acatcgtttt tcacgatcta aatttgcttg aacaagcttt
    64801 tacccattca tcctatgtga atgagcatcg caatctgcaa ttatcggata atgagcgact
    64861 tgaattttta ggagatgccg ttttagaact gatggtttct cagtatttat tccgtttata
    64921 tcctcatttg ccagaaggaa aattgacaaa aacacgagct gcgatcgttc gcgaagacag
    64981 cttgtcgaaa tttgccaaag aatgtcactt tgatcaatat gttttgttgg gcaagggaga
    65041 agagaattca ggaggacgta cgcgaccagc attgctttgc gacctattcg aagctttttt
    65101 aggcgcgtta tatcttgacc agggattcga cacgacccat gcattcatcg aaaaagtgat
    65161 ttttcctaaa gtcaaagcag gtgctttttc acatgagatg gatcataaaa caaaactaca
    65221 agaagtcttg caaaaatcag gtgatgtatc aatcgaatac cgattgatca atgaagaagg
    65281 tccagctcat gaacgagtat tttggatcga agtttatgta gatgatcaat tgattggcac
    65341 tggacaaggg aaatcgaaaa aattagcaga acaagctgcg gcggaaaatg cattggcagc
    65401 actgtaaaag taagtgaggg aaagctttgt atttgaaacg aattgaaata gccggtttta
    65461 aatcatttgc agacaagaca gtcattgatt ttgaaaatag cgtcacagca gtggttggac
    65521 caaatggcag cggaaaaagc aatattacag aggctatccg ctgggtatta ggagaacaat
    65581 ctgctaaaag tctgcgaggc ggaaaaatgc cggatatcat ctttgccggt tccgataccc
    65641 gtaagcagct gaatattgca gaagtcacag tgattttaga caatagtgat cattatctgc
    65701 cattggagta caatgagatc agtgtaaccc gccgttatcg acggactggg gaaagcgagt
    65761 ttttcatcaa taaacaaagc tgcagattaa aagatatcca agaattgttc ttagattctg
    65821 gattaggaaa agaatcgttc tcaatcattt ctcaaggaaa agtagaagcg attttcagta
    65881 gtaaaccgga agaccgtcgt gggatctttg aagaagcagc tggtgtcttg aaatacaagc
    65941 agcggaaaaa gaaagcagaa cagaagcttt ttgaaacaga agacaacctc agccgcgtcc
    66001 aagacatcat ccacgaattg gaagaacagt taactccttt ggccgcccag agtgaggcag
    66061 caaaggagtt tttacggcta aaagaaacac tgactcaaac cgatgtctct ttgatggttg
    66121 ctgaaatcaa gacagcaaaa aaagattggg acaacaaaca agcacagctt gcgaaattca
    66181 atctagaatt aggcaaacta tctgagtcga tccaagaaca agaatccatt ttggcgaagc
    66241 agcgtaaaga aaatgcgcaa gccgatcgtc tgatcgaaaa aaatcagcaa gtattattag
    66301 atctttcgga aaaactgaag cagacagaag gacaaaaaga tgttttgcag gagcggacaa
    66361 agcatacaca aaaaagctcg caagaatatc aaacttcatt agcagaagca caaaaaaagg
    66421 ttaaacattt tgaaaagctt caagaaagct tgatgaaagc agcagcagaa aaagaaacag
    66481 agatccaaaa agctgaagca aatctcataa aaacacagca agaattagaa aaataccaaa
    66541 aatcaacgaa agaattattg gcagaattaa gagatcaata tgttgatttg atgcaagaac
    66601 aagcagctgt aggaaacgaa ctgaagtatc tagaacgtca atatatacaa gaaacagcaa
    66661 aaagcaagca gacgttagcc aagcaatcgg aagtagaagc atcagtagac cgattgatct
    66721 tacaaaaaga agaattgaca caaaagcagg cgcagctaaa atcatcttta actgaaacaa
    66781 aagagaagtt agaaatgatc caacaaaacg ggaaaaagtt ccaagaagca ttagcaaaag
    66841 aacagcctaa aatgtatcaa ttaatgaatc aagtccagca gctacgtgct cgtaagaaaa
    66901 gccttcaaga aatccaagaa aactattttg gtttttacca aggggttcga ttggtcttgc
    66961 agcataaaca gcagctttcg ggtattgtcg gagcagtggc tgaactgatc gatgttcctg
    67021 cagatttcac gttagctatc gaaacagctt taggtggggc agcccagcat gtcatcgttg
    67081 aaaatgaaaa agatgctcgg caagccatca cgtatttgaa acagcaaaga ggaggaagag
    67141 caacattcct tccactgacg acaatcaaac caagacaatt accggctcat atattgacac
    67201 aagcagcagc tgtagaaggg tttataggga ttgccagtga acaagtcagt tatcccgatc
    67261 aaatccaaac tgtggttcaa aacctgctag gaacgatcct tttagccaaa gacttaacta
    67321 gtgccaatgc aatcgcgcaa acgatccgct atcaatatcg agtggtttcc ttagaaggtg
    67381 atgtgatgaa tgccggagga tccatgacag gtggagcgaa taaacgagga aaccaaggca
    67441 gtttgtttgt acaaaatcaa gaattgaaac agttgactag tgaatttgaa gaagcagata
    67501 aacaactcca agcacaagaa aaaaaagttc aggagctgca acaagaaaca gctcgtttag
    67561 ccgaagaaca agaagttttg cgtacacgag gcgaacaatt acgttttgaa gaacaagaag
    67621 cgacaaatca gctacaaaac atcataaatg aacttgaacg ttttgaaaaa gaaaagcaaa
    67681 tctcaacttt tgagacacgt gaactgcagc agtttatcga agattacgaa aagcaaacga
    67741 acgaattgaa agacaaacaa acagatcttg aatcgcaacg acaaaaaatc gacgaagaaa
    67801 tcaaatcgtt aagtcaagaa agcgaccaga tggaagccag aagagcacaa gtacaaagtc
    67861 aaaaagcaca agaacaagca gatcttgctg tcttgaaaga gcagtttaat catctgcaga
    67921 tccagctccg cggtgcccga gtccaaaaag cagaggcaac cgaacgtcaa gaagcgatag
    67981 aaaagcagtt ggcgacattg acagcagact tttctgatca tgaagtcaca gaagaaagtt
    68041 tggaaaaaca aattaacgaa ttatctgctc agcgagaaac attaaaagcc gagttggcaa
    68101 aagccaaaga gcagcgagat agaaggcaaa aggagatcga taagcttgaa gcagtcttgg
    68161 cagagcgtaa tcgtgagcaa aaagcacgac tgtcggaaca atccaaatta gaagtccaaa
    68221 aagaccgagc tgaaatgatt ttagataatc atttgaccta tctgcagtca gaatatcagc
    68281 tgacatttga gaaagcttcc caagactatc aagaaacaac cgatatcgaa gacagtagaa
    68341 caaaggtttc atccttaaaa gaacagattg aaaaactggg accagtaaac ttaaattcaa
    68401 tcgaacaata cgaacaagtc agtgaaagac atacattctt agcaacacag cgagatgatc
    68461 tgttagcagc gaaaaaccaa ttgtttgaaa cgatggatga gatggatgat gaagtgcgga
    68521 caagattcaa agaagtattt gaagctatcc gtcaagaatt caaaattgtc tttccaaata
    68581 tgtttggcgg cggtaaagct gaattagtac tgacggaacc aactgatttg ctgaaaacag
    68641 gtattgagat tgaagtacaa cctcctggca aaaaactgca aagtctaagt ttgctttctg
    68701 gaggagaacg agcacttaca gccatcgcgt tgctcttttc cattatccgt gtctgtcctg
    68761 ttccgttttg tatcttagat gaggtagaag cagcgttaga tgaagccaac gtgaaacgtt
    68821 ttggtcgtta cttgagcgat ttccaagatg acacacaatt tatcgtagtc acacaccgca
    68881 aaggaacgat ggtcgcagca gatgtactgt acggagtgac gatgcaggaa tccggtgtat
    68941 caaaaatcgt ttctgtacgg atggaagata tcaatgaaga ggcgaaaatc aacgaaacga
    69001 aagtgtagga acgaaaatga tcaaattagt agcaatcgat ttggacggta cattattaaa
    69061 tgaacaaaag ctgatttcag atgagaataa acaagcatta gcacaagcaa aacagcaagg
    69121 ggtcaagatc gttctatgta caggacgccc cttagctgcg atggcgcatt atctgcaaga
    69181 attaggactg gtagatgaag gtgactacag catcactttc aacggcggtt tggtacaaaa
    69241 aaatgatact ggtgaaatca tggaaaagaa agtgatggaa gtatcagata tccaacgctt
    69301 atacaaactg gctcagcaat tggatctgcc actagatgtt ttgtctgaca atgtggtttt
    69361 acagctgcct agtgccccaa ggaagcaatc cttgtacaat gtgttgaata agctgctgca
    69421 attccaacca gcggatataa gcgatattac agatgaattt gtgcttaata aggcagtaat
    69481 cgcttatgac caagaagaat tagatcctaa aatcaaagaa ataccagcag aattccatga
    69541 aatgtatgag atcatcaaaa caagaagtat gcttttggaa tttatgccaa aaggtgttac
    69601 aaaagcttat ggtatttctt tattggcaaa agatcttggt ttgaaacaag aagaaatcat
    69661 ggcgatcggt gatgaggaaa atgatttacc gatgatccaa tatgctggac taggcgtagc
    69721 gatggagaat gctgtgcctt tcgtcaaaga agcagcagat ttcgtgacag ccagcaatga
    69781 agaaaatggc gtagccaaag tgatccaaaa atttgtatta gagtaggggg agtcaaatgg
    69841 gattttttga taaaatcaag cgtgctttta ctggcgaaga gaaaacagaa gaagttgaag
    69901 tacaggagaa gtacgacaag ggtttagaaa agacgcgtaa aacatttggt gaacggatga
    69961 atgagttgtt tgccaatttt cgaacagtcg atgaagactt ttttgaagaa ttagaagaaa
    70021 cacttatcgg tgcagatgta ggttttgaaa cagcgatacg aattaccgaa gcattacgtc
    70081 aagaagtgaa actcagaaat gcgaaaaaat cggcagaagt ccaaaatgcg atcatcgaga
    70141 aacttgtgga tctctatgaa gcggaaggaa tcaatgaagt aaatgaacta aatatccaag
    70201 aagacggact aacagttatt ttgtttgtcg gtgttaatgg tgtagggaaa acaacaagta
    70261 ttggtaaact tgcccatcag ttcaaaaacg aaggtaaaaa agtattgatg gcagcagcag
    70321 ataccttccg cgctggagca atcgaccagt tagttgtctg gggcgaacgc gctggtgttg
    70381 aagttgttcg tggcaatgca ggaggcgatc cagcagctgt cgtattcgat gctttggaaa
    70441 gagcaaagac agagcaggca gatgttttac tagttgatac tgcaggccga ctgcaaaata
    70501 aagtgaactt gatgaacgaa ttagaaaaaa taaaacgagt aatccagcgg gaattaccag
    70561 aagcacccca agaaacactt cttgtattag atgcaaccac tggtcaaaac gcgatggttc
    70621 aagcaaaaca attcaaagaa acaacagatg tgaccggtct tgttttgaca aaattagatg
    70681 gtacagcaaa gggcggtatc gtacttgcta tccgtaatga attgcattta ccagtgaaat
    70741 tggtcggtct tggtgaagga atcgatgact tggagccgtt tgatccaaat gattttgtcg
    70801 ttggattgtt caaaggatta ctaaaagaag aataacccat gtctaaaaag caggagctgt
    70861 gacaaaagtt gcagctcctt gtttgaatga ttgtttttaa catagctgtg tttttgatgc
    70921 cttcattttt atacttattt gtaagcggtt ctcaaacaat ttcgttcttt ttcttagact
    70981 ttttgattat aattagattc atatgaattg gaggtcactt aatgatgaat tttaagcaat
    71041 tattattgca tgtcaatgcg cgccctttta tcgaccaagc tcgatttggt atagaacggg
    71101 aaggacagcg ggttgatctt gcaggaaatc tagcaaaaac cgatcatcca gcaatctttg
    71161 gcgatcgatc ctatcacccc tatatccaaa cagattttag cgaaacacaa acagagatga
    71221 tcacccctgt taccgattct attcccgaat tatttcagta tctcgctgct gtttatgatg
    71281 tgactgctcg ttctataccg aaagaagaga tgatctggcc attaagtatg ccacctgcct
    71341 taccggaaaa agacgaagag atcattattg caaaattaaa aaatttcgaa gatgtcttgt
    71401 atcgacgtta tttagcaaaa gaatacggga agcgaaaaca gatggtaagc ggtattcatt
    71461 tcaattttga atttggcgat gagttgctaa gaacattgtt cagccatcag gaagaatttc
    71521 aagatttttc cgaattcaaa acagaacttt atttgaaaac agcgagaaac tttatgcgct
    71581 atcgctggat gatcacttat ttattcggtg cttcaccaat gagtgagaag aactactttt
    71641 tagatgaatc acatccgcaa gaacctgttc gcagtattcg caacagtgcg ctgggctata
    71701 cgaatcatcc aaatgtgaaa gtttcctatg cttctatgaa acagtattta gcagatatcg
    71761 agcgaatgat cgaagaaggg aaactttcgg aggagaaaga attttataca ccgcttcgtt
    71821 tccgaggcgg aaagaaagtc gcagatttag caacaacagg tgttcgttat attgaattgc
    71881 ggaatatcga tttgaatcct tatgcaagat tgggtatcaa tccagagcaa gttcggttct
    71941 tacaattatt cctgatgtac atgttatgga cagaagaaaa agaagactgt gaccaatggg
    72001 tagcagaagg tacgactcga aataacaaag tagcactaga acaaccatca gatcaaacag
    72061 aatttcatca agaaggtaga gaaattcttg aaggaatgaa gcaaatgctg gttgaattgg
    72121 attggttgga ttctctttat ttagtagagg aagcattgac tcaaatggat catcctgaac
    72181 aaacgttagc agcgaaactc tatcaagaag cacagctatc gagtcagcaa gaagttgctg
    72241 tggcattggg acatcaatat tataaagaaa gccatgaacg tccctatcag ttggccggtt
    72301 ttcgcgaaat ggaactttct actcagatat ttatgttcga tgccatccaa aaaggtgtcc
    72361 aagtcaaagt attagatgag tcggatcaat ttttgcgtct gcaattccaa gaccatgtag
    72421 aatatgtgaa aaatgcaaac atgacaagta aggacagcta tatcgtccca cttatcatgg
    72481 aaaacaaaac agtaacgaaa aaagtcttaa aagaagctgg gttccgggta ccaggaggcg
    72541 cagaattttc atccatggaa gaagcggtaa aagcttatcc gagatttgcc gagcaggcat
    72601 ttgtgatcaa acctaaatca accaattacg ggttaggtat cacgattttc aaggaaggag
    72661 ctagtctgga agattatcaa gcaggtttag ccattgcttt tcgtgaagac agttcggttt
    72721 tagtagaaga atttatgccg ggaacggaat atcgtttctt tgtgatcgac ggagaagtcc
    72781 aagccatcat gttgcgagtc cctgctaatg ttattggtga cagtatccga acagtgaaag
    72841 aacttgttga agaaaaaaat agtgaccctt tgcgtggaac gaatcatcgt gcacctttag
    72901 aattgatcca attaggtgaa ttagaacaat tgatgttgaa agaacaaggg ttgacgatcg
    72961 aatcagttcc ccaagccaat caaattgttt atctaaggga gaactcaaat atcagtacag
    73021 gtggtgattc gatcgatatg actgatgaat tttctgaagc ttacaaaaaa atcgccgttt
    73081 ctgcggtaga agcactagga gctaagatta gtggtatcga tttgattata ccagataaag
    73141 aaatcgatcc tacaactgat aaaaaagcat atgggatcat cgaagcaaat ttcaatccag
    73201 ccatgcatat gcatgtttat ccttttgcag gaaaaggcag aagattgacg atgaatgtat
    73261 tgaaattgtt atatcctgaa gtattttaaa agaaaaagct tttgacagat tgtcctattt
    73321 gatgtcaatc tgatcaaaag ctttttttgc atgtaaaaat ttatgtgttt tttcgtttta
    73381 aaataaacga tcccagaagc tttttttctt ttttggtttt tctttttcag ctggcgaaga
    73441 gttttcttct gggtattttt tttcgatatc aatcacaggt tcatttactg cttcttttgc
    73501 tgttaagagc aatccgaatt cctcaggatt tgcagtgacg taatcgttga ctacagtgaa
    73561 acggctattg tttttcgttg ctagctggat atattggttt tggacagaaa ggggtagagc
    73621 cccattcaag agtatggaag catctggatg ttttttgagt tcttttaaaa agttttcttt
    73681 gttttttggt tggatcattt cagcaacagt catactaaga tagcagcgtt ccctgaatgt
    73741 ccctaaatat ttatgctgtt cttcagggtt cactaaaggt gtaccataaa gtcccttatc
    73801 caaatggctt tttacatcat cttgtgccat aaaaaatacc ctccttcttc tctttgtctt
    73861 tcttacgatt atagcatatt cccaataata cctaaaacat atgaaaaagc ggttgctttt
    73921 gttcaacgat tcacttaaaa taagaatgaa aatcattctc aattagaaag gtggattgga
    73981 tgcgctacgg acgactagta gcattattag ttattttaag tagtatttca ttgttcatcg
    74041 gagtatcgaa tgtacaattg tcagaactgc tccagttaga tgggcaaagc ttacaaatct
    74101 tgctagtgac tcgtttgcca cgaacgattt gtttgatcct tgtgggagcg accagtagta
    74161 tttgtgggtt gatcatgcaa catttgaccc aaaataaatt tgtttcacca acgactgcgg
    74221 gcacgatgga tagtgcgcgt ttaggtattt tagtggctat gattttccta cctggtgcat
    74281 ccttgattgt ccgatccttg acagcatttt gctttgcttt tgcaggaaca ttgctttttt
    74341 tatcccttac gcgattgttc ctccagaaaa atcaattgat gcttccgttg attggagtca
    74401 tgttagggaa tatcattggt tcagttgcta ctttttttgc ttatcaattc cagttggtac
    74461 aaaatatgac ttcatggctt caagggaatt ttgcgactgt gatacgagga aattatgagt
    74521 tattgtatgc tgttgttcca ttgttgctct tggtttggct atttgcttac cgatttacga
    74581 tcattggtct gggcgaatcg tttgcagtaa atctgggaat agattaccag aagatgcagt
    74641 ttttaggcat tggcttagta tcattggcta gtgcagtcat gttgatcatg gtaggaagca
    74701 ttccttttat cggagtgatc attccgaatc ttgtctctag aatatatgga gatcaagtgc
    74761 acaaaacaat tggcgtaact gctttagcag gtagtttgtt cttagtattt tgcgatcttg
    74821 tcgctcgcgt gttgattttt ccttatgaaa taccagtcag tgtgatcgtt ggaatcatcg
    74881 gtggggctat cttcctttat cttctgataa ggggacgacg atatgcttaa aaaaaagcca
    74941 tctattttta ttggtatgct tgttcttgtt ttatttgtta caggaatgta cttgacgatc
    75001 catacatatg gaaactggca ttttgcatgt gcattgagga gtaagaaaat ttttgcattt
    75061 atccttgtct cgctagctgt cagcagtgcc acaatcagct ttcagacaat tacgcataac
    75121 cagtttttga ctccttcagt attgggactt gagtcgttat atgtccttgt ccaaacggtc
    75181 cttttcttct ttatcggagg agtgacgatg cttcagcaga caggtacagt ttttttctta
    75241 ttgaacgttg ggctgatggt cgggctgagt ctattgttat ttttctttct tttgaaaaaa
    75301 atgagagcga atcttttttt attactgatg tcagggatga ttttaggtac gctttttggt
    75361 agtgtcagta cctttttaca agtcatcatg gatccgaatg aatatgattt gcttcaaggc
    75421 agattatttg caagctttgg caatatccag agtcagtatc ttttgcctgc ttctttgatc
    75481 attatcggct gctggtacct gttatttcga tatcgacatg tgctagacgt tttccatctc
    75541 ggaaatgaac aagcagtaaa cctcgggata gacacagatc gttttgaatt tttgatttta
    75601 tgttgtgtca gtcttttgac agggacatca acggctcttg tgggtccagt tactttttta
    75661 gggtttattg tcgcaaatat cagttatcgt gtcttttcta cttatcgtca tggtcagcta
    75721 tttatcggaa gctttctggt agctatgctg tttctgacag gaggacagct cgcagtagag
    75781 cagattttcg gctggaatac aacagtgagt gtcgtcgttg aatttgtcgg aggaatttat
    75841 tttgtcacaa aattgattcg cgaaaggaag agtatcctat gattgattta caaaaggtca
    75901 ataaactgta ccatggaaaa tcagtggtta aagatgtaca gttaacgatt cctgaaaaga
    75961 aattgactgc ttttatcggt ccaaatggtg ctggaaagtc aacggtgttg tcgatgatca
    76021 gtcgtttgat accaaaagat acaggtgaaa tctatttaga ccataacgaa gtaaaggctt
    76081 ggaaatccaa tgaattagcc aaaaaattat cgatcctacg acaatcgaat gaaataaatg
    76141 tccaaatcac cgtgaaagaa ttagtagaat ttggacgatt tccttatacg aaaggaaaat
    76201 tgacgacaaa agacgaggaa atcgttcagg aatcactgga acaccttggg ttagaagaaa
    76261 tagcggatca atcgattgac actctttcag gcggtcaact ccaacgtgcg tatattgcaa
    76321 tggtgctagc gcaagacacg gattatattt tactggatga acctttgaac aatctagata
    76381 tgaattttgc ggttcaaatc atgcagattt taaaaaattt agtacaagaa atcgggaaaa
    76441 caattgttat tgttcttcat gatatcaact ttgctgcaag ttatgcagac gaaattgttg
    76501 caatgaaaga tggacagtta tttacgcaag gaacgaccca agaaatgata aaaaaagatg
    76561 tattggatga attatacgaa atggacatac ggatttgtga gattgaaggc cgcagattct
    76621 gtctttattt ctaaaactaa cattaggaga gaagagatga aaaaattagt agtaggtatc
    76681 ttgattctag catcatttgg tttagcagca tgtggaaata gcaacaacac cagtcaggca
    76741 gacacaaaaa aaagcagcac acaaactgaa actacattga cgataacaga tagtaatgga
    76801 gaccaaatcg aggtgccaaa taatccagaa aaagtcgtcg tattcgacaa tggttcttta
    76861 gatacgatgg atgctcttgg tgttggagat aaggtagttg gagcagctac aagcagcctt
    76921 cctgagtatc tttcatccta taaaaaagta gaatcagcag gtggaataaa agaaccagac
    76981 ttagaaaaaa ttaatcagct tcagccagat ctaatcatca tttctggccg acaaagagat
    77041 tttcaaaaag atctaagtca aattgctccg accattttcc tttctttaga tgcgaaaaat
    77101 ccatgggaat ctttccagca aaatgtcact gctttagggg agatctttgg taagcaagaa
    77161 gaagctaaaa ctcaattaga ggaattatct agtgcaatcg atcagacgaa aaagaaagca
    77221 gaagctactg acaaaaaagc actagtcaca cttgtgaatg aagggcaatt atcagcctac
    77281 ggatctggtt ctagattcgg tttcatccat gatttatttg gctttgaaca agcagatgat
    77341 caaatcgaag catccactca cggtcaaagt gtttcttatg aatatgtgct ggaaaaaaat
    77401 ccggatatct tatttgtagt agacagaaca aaagctattg gtggagacga cagcaaagat
    77461 gatatttctg ccaatgaact ggtagcacaa acgaatgctg gtaaaaatca gcagatcatc
    77521 tcccttgaac cagatgtctg gtatttaagc gggggcggtt tagaatcaat gaaactgatg
    77581 atcgaagatg tgaatcaagc atttaaatag cataaataac agaggattaa actctcatac
    77641 tatgtaacaa taatgtttct tacattctta aatagcaaga gcctatcctc aaagaagtta
    77701 tcaaaaacca tccgtaaaat cgtattgatg attttacgga tggtttttat ttatttgaga
    77761 aatctttttt caacgtgaat tcattatcta catcatacgg gttgattcta gaatggggag
    77821 tggcaaaaaa tagtgtgatc cattttttca gcatcaagat cgcctccttt gatttgaatc
    77881 aaaggtacaa agcgaaactt agataaaact taaatttaga tatttttatt ttgtaccgat
    77941 ctcttcttta aacgatgttc catctgctgc ttctcggcaa taag
//
LOCUS       DACGLE010000005        72557 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0534
            SAMEA3304449-rid9560453.denovo.005, whole genome shotgun sequence.
ACCESSION   DACGLE010000005 DACGLE010000000
VERSION     DACGLE010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304449
            Sequence Read Archive: ERR985064
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 72557)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 72557)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702225.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702225.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 20:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,045
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 27 of 67
            Pseudo Genes (incomplete)         :: 25 of 67
            Pseudo Genes (internal stop)      :: 19 of 67
            Pseudo Genes (multiple problems)  :: 4 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..72557
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304449-rid9560453.denovo.005"
                     /strain="VREN0534"
                     /db_xref="taxon:1352"
     gene            complement(186..470)
                     /locus_tag="IXT20_000419"
     CDS             complement(186..470)
                     /locus_tag="IXT20_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4858896.1"
                     /translation="MKYYDVTFHELSGKTVVKRDIPSEKNGFDVWKDACADYNENELF
                     ILINDGAYVTMNRKFIVRIDTEEVEDPTEKARSRKDEIMGVVNTLSNMGF"
     gene            complement(488..1378)
                     /locus_tag="IXT20_000420"
     CDS             complement(488..1378)
                     /locus_tag="IXT20_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HAQ4858897.1"
                     /translation="MKSMTGFGKAIRETKAYQLDIEIKSVNQRFLDVQIRSPKILNFV
                     ENDFRQLIKQQLSRGRIEVFVNLAYIGKNEKQIVINWELIDSLITQLTDGIQQRYGSQ
                     NQVSISRMAERFALDENFVTVEEKPEESMEELAQLALDTLKSALDSIEASREKEGQAL
                     ALVLKKNTEEFKEVLKKLDSFVEIYEKEFQIRYQKKLEEFLGAKVDEQRLLTELAILL
                     ERGDIHEELDRLAIHVQKLDELIEAQCPVGRELDFLIQEMNREINTIGSKSNAIEIKN
                     QVVQSKTILEKIREQVQNIE"
     gene            complement(1398..1616)
                     /locus_tag="IXT20_000421"
     CDS             complement(1398..1616)
                     /locus_tag="IXT20_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4858898.1"
                     /translation="MAQEFSYEIVEEIAILSENNKGWRKELNLVSWNGRPPKFDLRDW
                     APDHEKMGKGLTLTNEEFEQLQKAIENM"
     gene            complement(1586..3286)
                     /locus_tag="IXT20_000422"
     CDS             complement(1586..3286)
                     /locus_tag="IXT20_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HAQ4858899.1"
                     /translation="MEIKKVYFSQKMSLYSPIQQAKQPLPFFQSHQESRDAFWRSAKK
                     QCAFKAVSKKYTVYFTIAKSRGDVIFNNLLIEGETFEWKKFFRYMEACARFFIKENCC
                     FLFQSPLSEEWLRIFHKNGYQGTTQLNKKLVYHTALVLGGGGAHGAYQIGVWQALKEH
                     DINFEIITGTSVGALNGALILQGDMKKAVNLWKKLSTRQVLALPEMAAVEDLRERFYR
                     ERRQMTKTALIEGGVSSAPLEKMVKDNLDLSLLKHNPKIRLYTVSTKLPELAEVVTDI
                     QQTKTEEIPDWILASASFYPAMAYRKIGKNKYADGGYRNKIPIDIAINKGATEAFVVD
                     VQGPGPAKKIRMPDIFIHWKCQTLWTLGSFLLFDSQRNQLNLQLGYLEMKKRLGYYYG
                     NWYTFHSVKPARTYWRGFLSYLTKEIQLELSFFKSIKFWQKLRNLYKNRVVPETCGLA
                     MLELLAKKHFLLPNEIYQVDMMIQMICQQDIKSMPDDLLAQIGQLSTEEWRRYRKYQQ
                     KIQNERTKTVYFDYLLQGKRKDRLQHELLKQPVDTLLILYLYYLKEEQPWHKNFHMKS
                     "
     gene            3553..4326
                     /locus_tag="IXT20_000423"
     CDS             3553..4326
                     /locus_tag="IXT20_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4858900.1"
                     /translation="MTESVYVHIDTTSNAVLTKGLTATDFANSIVHFPQNLLLLDPSA
                     SAGEYESHTGLKVIRGTENIQRFFSSSRNRRGFDELKWIDFTDLTMLKELTPLEISEL
                     LYFGHMKTHLHSPFFYKLQNNFVYFDLNDQLNRIYYRYLDEFYRIFASKLSSILSDKI
                     NVKKSFFKKETPVEKISVELLKEMRNLMQEGIIFSFDQVGLIDGKYRIPIYVIEDYIW
                     KLKGTRYREEEIIATLIYSVTDRHWQLVEDADLSYLPLK"
     gene            complement(4407..4976)
                     /gene="thiT"
                     /locus_tag="IXT20_000424"
     CDS             complement(4407..4976)
                     /gene="thiT"
                     /locus_tag="IXT20_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupled thiamine transporter ThiT"
                     /protein_id="HAQ4858901.1"
                     /translation="MGKNRVWVEGTIVAALAMVLSLIPIQIGSSFSISLGQIPLTLFA
                     LRRGTKAGMLAGFIWGILHFPLGQVYYLSVVQVLIEYPIAYTFAGAAGLMASNVQKGY
                     VDDNTKQIRKSIVFGALIGALSRYFWHFIAGVVFWGAYALWGMNPWLFSFVMNGASGL
                     STAIVTIIVLLGIQQTTPSIFVPDKRQEL"
     regulatory      complement(5042..5144)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(5150..6121)
                     /locus_tag="IXT20_000425"
     CDS             complement(5150..6121)
                     /locus_tag="IXT20_000425"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016618611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HAQ4858902.1"
                     /translation="MSKHYFDPRLKIFALNSNRPLAEKIAAAVGVELGKSSVTQFSDG
                     EIQVNIEESIRGSHVYVIQSTSSPVNDNLMELLIMIDALKRASAKTINVVMPYYGYAR
                     QDRKARAREPITAKLVANMIQKAGATRMLTLDLHAVQIQGFFDIPVDHLMGAPLIANY
                     FLEKGIKGDDVVVVSPDHGGVTRARKLAEFLKSPIAIIDKRRPKANVAEVMNIIGQVK
                     GKTCVLIDDMIDTAGTITLAANALKEAGATSVYASCTHPVLSGPALQRIEDSAIERLV
                     VTDSIYLPEDRKIDKIDEVSVGGLMGDAIKRIHENKPVSPLFETKNK"
     gene            complement(6215..7588)
                     /gene="glmU"
                     /locus_tag="IXT20_000426"
     CDS             complement(6215..7588)
                     /gene="glmU"
                     /locus_tag="IXT20_000426"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HAQ4858903.1"
                     /translation="MDARYAIILAAGKGTRMKSKLYKVLHPVSGKPMVEHIINRVSET
                     KPDEVITIVGHGAEQVKAQLGERSKYALQAEQLGTGHAVLQAASFLEGKKGTTLVISG
                     DTPLLTTETLNNLFEYHQGKNASATILTAQAENPTGYGRIIRDHIGIVEKIVEQKDAT
                     PEEALVQEINTGTYCFDNEALFDALSKVGTNNAQGEYYLTDIIEILKEEGHTVAAYQT
                     DDFEESMGVNDRIALAKANEIMRKRINQMHMVNGVSFVDSATTYIDAGVEIGPDTLIE
                     AGVQIQGNTVIGSDCVIGSHSKIVDSRIEDHVVIENSVIESSHVKKHADVGPYAHLRP
                     KAEIGENVHIGNFVEVKNAQIGKGTKVGHLTYVGDATLGEEINVGCGVVFVNYDGKNK
                     HHTTVGDHSFIGSSTNIIGPVEVAKNSSIAAGSTITDNIPEYALAIARARQVNKEGYA
                     KKLPYLN"
     gene            complement(8091..8909)
                     /gene="purR"
                     /locus_tag="IXT20_000427"
     CDS             complement(8091..8909)
                     /gene="purR"
                     /locus_tag="IXT20_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HAQ4858904.1"
                     /translation="MKVRRSERLVDMTQYLLNHPHELISLTSFAERYESAKSSISEDL
                     GIIKKTFKERGYGTLETIPGAAGGVLFIPEISYEEAKKYIESLAERLSEQDRLLPGGY
                     VYLSDLLGEPELLRQVGRIIASKYLGEKIDAVMTVATKGVPIAQAVSYYLNAPFVIVR
                     RDSKITEGSTVSVNYVSGSSERIEKMELSKRSLKRGSRVLVVDDFMKGGGTVNGMKSL
                     IEEFESELVGITVFAESKFNGHRAIDDYTSLLYVKDVDTQTKNITVVPGNYFSE"
     gene            complement(9076..9900)
                     /locus_tag="IXT20_000428"
     CDS             complement(9076..9900)
                     /locus_tag="IXT20_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HAQ4858905.1"
                     /translation="MALLSYEFMRRAFLAAIFIAGIAPMLGVFLVIRRQSLMADTLSH
                     VSLAGVALGFFLNLNPTLTTMLVVVLAAVILEYLRTIYRTYSEISIAILMAGGLALAL
                     VLMNLSGGNSATSIQSYLFGSIVTITWEQVIFLGILFVAIGILFFLFKRPMYVLTFDE
                     DTAHVDGLPVHLMSMMFNVITGVAIAVMIPIAGALLISAIMVLPAAIGMRLGKSFNVV
                     IAISVCVGFLGMLSGLTSSFYLDTPPGATITLVFIALFLIVNIIKRILVAFRRKNN"
     gene            complement(9873..10553)
                     /locus_tag="IXT20_000429"
     CDS             complement(9873..10553)
                     /locus_tag="IXT20_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HAQ4858906.1"
                     /translation="MRYIEISDLTFYYDDEPVLEDVSYHVDAGEFVILTGENGAAKST
                     LVKASLGLLKPSKGMIKIAEKNQEGKKLSIGYIPQQVASFNAGFPSTVIELVRSGRYP
                     RGRWFKRLSEKDHQHVQRALEAVGMWDMRNRRIGELSGGQKQRISLARIFATDPDLFV
                     LDEPTTGMDEESRNEFYRLLRHSAHVHGKSVLMITHDHEDIKQYADRQIRLVRKEDSQ
                     WRCFHMNS"
     gene            complement(10576..11514)
                     /locus_tag="IXT20_000430"
     CDS             complement(10576..11514)
                     /locus_tag="IXT20_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HAQ4858907.1"
                     /translation="MKKYLLTATVMIGALLFAACGNTNKEADKKEDLTIVTTFYPMYD
                     FTKEIVGDEGNVKLLIPAGTEPHDFEPSAKERAEISDADVFVYNSSDMEFFVDSLKDS
                     VDSKQTLMIEAAKGIDRLESQEADEHEESEEGHGHSHEYDPHVWLDPVLAIKEVRTIA
                     EELGEKYPDKKETFTKNADAYIKKLEALDQKYSDELKDATNRTFVTQHAAFAYLANQY
                     DLKQVAISGVSPDQEPTPSRLAELKEFVKKNNIKVIYFEENASSKVAETLSNETGVKL
                     EVLNPLESLTNEQIKAGENYISVMEKNLKALKESIN"
     gene            complement(11759..12610)
                     /locus_tag="IXT20_000431"
     CDS             complement(11759..12610)
                     /locus_tag="IXT20_000431"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HAQ4858908.1"
                     /translation="MELIEKAPAKINLGLDVLYRRQDGYHELEMVMSSVDLADHLFFE
                     EITEDKIIVETNKAFLPVDERNNVYQAALLLKNKYNIKTGVKIFVKKNIPVAAGLGGG
                     STDCAAALRGINQLWNLGCSLEELAEIGLEVGTDVPYCVYGSTAFVGGKGEEIQVLPS
                     MPQCWVVLVKPRMSVSTRTVFREVDTQAIQHQDIHGLAEAIREQDYQQMIKKMGNSLE
                     DITGKRHPVIHQIKERMIKYGADAALMSGSGPTVFALCQHYSRAQRVYNGLKGFCEEV
                     YLVRTLQ"
     gene            complement(12764..15337)
                     /gene="mprF"
                     /locus_tag="IXT20_000432"
     CDS             complement(12764..15337)
                     /gene="mprF"
                     /locus_tag="IXT20_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HAQ4858909.1"
                     /translation="MKNKRYLMLQWIKEHSLILKLIFFGSVLVFVANQVANIANGMSW
                     QDIWQRMGQQSRMTILLMILAGLLGVTPMLLYDWVTIRVLEKQGKPKMERKEFLLAAW
                     VTNTINNLAGFGGVVGASLRASFYGKQTNRKMVLATVSKVALFMLTGLSVWSFLTFID
                     VFFIQSESIFRSYWIWLFGGSFLAPVIFFFAYLKRKTLFFEFYPKGLLGLLLASFGQW
                     TGALAVFLIIGKLMRIDISMLSIYPMFMIATLIGMLTMVPGGMGTFDVLMILGMSQLP
                     VRQDVAVVWLLYYRLFYYVLPFMTGLLLFITHTGVKINRFLDNIPRLFFQKVAHIVLV
                     SAVYFAGIMMVLLSTITNLSAISRLFQVLLPFSFNFLDQTVNLLVGFLLLGLARALSV
                     KVKKAFLPTIALLSFGILNTITRTLSWQLILVYVLILLAVWLSRKEFYRTKFVYSWGA
                     IIFDASLFGFLFIVYAIAGYHSGRFLVGRITGNHFILFPSDDVWFSGLIGLGISLLAL
                     VALYQYLAEDTTKLGSEYEKDRFDRLLETYGGTATSHQLRLSGYSYFYYQEDDQDQVV
                     FGYQIKGNKCFVLGDPVGNPKMMRAATNQFMKQADCLGYQLAFYKISESYVVMLHELG
                     FHFTKVGEAGIVDLATEDLSRLSQQLEYQRLMKEGYHFTWYDQLPEELHQAVNEVSQK
                     WLQGAREKYFSVGRFDKSYVLSSGVGIVRKDQKVVGFITAQPMTKKQAGYDLLRVLPK
                     ESEELADYLLANLFIVYEKMGYSEANLGLAPLANVGETDFSFFQEKVMHIVYNYGNFF
                     YSFQSAYEKKLKYVDHWEGRYFAYRKGSNFGFATMQLLSLIGKEKGNVPSLAEEVLTE
                     L"
     gene            15478..16284
                     /gene="proC"
                     /locus_tag="IXT20_000433"
     CDS             15478..16284
                     /gene="proC"
                     /locus_tag="IXT20_000433"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HAQ4858910.1"
                     /translation="MKVGFIGTGNMAQAIIDGLLKKGTVSKEEVFVSSGHFENAQKFA
                     AETGVHACKSNQEVASQSDIVLLAVKPKIIEKVLEELREYSQKVLFISIASGKSLTDL
                     ETALEDPQASIVRVMPNVNVSVGAGVSAICKNEAATEEQLQQATMIFEAIGSVYPLAE
                     SDFSTFIGLAGSSPAFVYMLIDAMGRTGVLNGLSKQTATEIAAKAIMGSCQKFLSSDK
                     NPWELVDQVSSPGGTTVAGVVALEEAGFIPAIIKGINTTIEKDLEMLNKK"
     gene            complement(16363..17919)
                     /gene="rny"
                     /locus_tag="IXT20_000434"
     CDS             complement(16363..17919)
                     /gene="rny"
                     /locus_tag="IXT20_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HAQ4858911.1"
                     /translation="MVFNILLAIIGLIVGLGLGFMVAKSRHEKEIAGAQSSAAGIIDS
                     AKKEAETLKKEALLEAKEENQKYRSEVESELRESKLELKSQENRLIQREQTLNRKDDS
                     LEKRENSLESKEEALSSKQQLIEEREKDVEKMIEDQQKELEKIAGLSKEDARDVIMKS
                     TEEELNHELTLMVKESEQRAKEEADRKAKNLLSLAIQRCAADQVSETTVSVVTLPNDE
                     MKGRIIGREGRNIRTLETLTGIDLIIDDTPEAVVLSGFDPIRREIARMTLEKLIQDGR
                     IHPARIEEMVEKSRKEMDERIREYGEEAAFEVGAHTLHPDLIKIIGRLRFRTSYGQNV
                     LKHSVEVAKLAGILAAELGEDIQLAKRAGLLHDIGKALDHEIEGSHVEIGAELAAKYK
                     ENPVVINAIASHHGDVEATSVISVLVAAADALSAARPGARSESLENYIRRLRSLENIS
                     NSFAGVESSYAVQAGREVRVMVKPEEISDLDAVRLVRDIRKRIEEELDYPGHIKVIVI
                     RETRATDYAK"
     gene            complement(18330..19379)
                     /gene="recA"
                     /locus_tag="IXT20_000435"
     CDS             complement(18330..19379)
                     /gene="recA"
                     /locus_tag="IXT20_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HAQ4858912.1"
                     /translation="MADDRKAALDAALKKIEKSYGKGSIMKLGEKIDQQISTIPSGSL
                     ALDVALGVGGYPRGRIIEVYGPESSGKTTVALHAIAEVQKNGGTAAFIDAEHALDPQY
                     AQKLGVNIDELLLSQPDTGEQGLEIADALVSSGAVDIVVVDSVAALVPRAEIDGEMGD
                     SHVGLQARLMSQALRKLSGSINKTKTIAIFINQIREKVGVMFGNPEITPGGRALKFYA
                     TIRLEVRRAEQLKQGTDIVGNRTKIKVVKNKVAPPFKIAEVDVMYGLGISQEGELLDM
                     AVEKDIVDKSGAWYSYKEDRIGQGRENAKIYMANHPEMMAEVSALVRAAYGIGEEVAV
                     PEDEKGQEELPLVEE"
     gene            complement(19488..20729)
                     /locus_tag="IXT20_000436"
     CDS             complement(19488..20729)
                     /locus_tag="IXT20_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HAQ4858913.1"
                     /translation="MKAEIIAVGTELLLGQVVNTNATFLSEQLADLGIEVYYQTVVGD
                     NQQRLEELIALAETRSELILLCGGLGPTEDDLTKEATAAHLGKSLIQNTEGYKKLLAY
                     FETTHRKMTKNNLQQSQIIEGGVPLPNRTGLALGTFYQTDTHAYILLPGPPNELKPMF
                     VEQVRPLLEERFPSEEKLISKVLRFYGIGESRLVTELKDLIETQINPTIAPYAKPNEV
                     TLRLTVKTNDVQAGNQALLALEEKIQERVGEYFYGYGDDNSLAKVVVELLKENKQTVT
                     AAESLTAGAFQAALGDIAGVSEVFPGGFVTYSLQTKAGFLEIDPELLAEYGTVSKECV
                     EQMAIQAREIARTDYSVAFTGVAGPDTLEGQSAGTVWIAISSKDGVVSEKYHFARDRA
                     AIRYNAVMQGFDLLRKQLLRK"
     gene            complement(20969..21547)
                     /gene="pgsA"
                     /locus_tag="IXT20_000437"
     CDS             complement(20969..21547)
                     /gene="pgsA"
                     /locus_tag="IXT20_000437"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010754872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HAQ4858914.1"
                     /translation="MNLPNKLTVLRICMIPIFILVVGLPLDWGQFNVGDTTLAVTQLV
                     GAVIFALASITDWLDGKIARARGLVTNFGKFADPLADKMLVMTAFIMLVDQGKAPGWV
                     VAIIVCRELAVTGLRLLLVEHGEVMAAAWPGKVKTATQMVAIILLFINNIPFSLIGIP
                     VDQIMLYACLIFTIYSGVDYFIKNSGVFKGSM"
     gene            complement(21756..22640)
                     /locus_tag="IXT20_000438"
     CDS             complement(21756..22640)
                     /locus_tag="IXT20_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ4858915.1"
                     /translation="MAFETIGEKLRQARLNKKISLDELQQITKIQKRYLEAIDNDDFD
                     QLPGKFYVRAFIRQYAEAVGEDGDHLVDVFDGKSKLTGSVPERPQPETVSTSRKAMHQ
                     EESHPSRFWTSLPVILLGLVALAIVVVVGYMTWQDRQADPIIGDTASSLKVEGSVEES
                     SSSEQSTQTKESSTVESTTKSSEEKKMAIATGEDSGSAIAVNITDATKPVTLEFTAVN
                     RVWLGVLVDNAYVYQGTLAANETQSTVLPETATNATITIGAASNATIKANGESVPVNP
                     GENNQSPKNVNLTLQYAE"
     gene            complement(23418..24704)
                     /locus_tag="IXT20_000439"
     CDS             complement(23418..24704)
                     /locus_tag="IXT20_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HAQ4858916.1"
                     /translation="MNKTEYEQINETLYHEVLPNGLTVYLLPKNDYHKTYGLFSTNYG
                     SIDNEFIPYGEKEKVKVPDGIAHFLEHKLFEKEDGDVFQLFGKQGASANAFTSFTKTS
                     YLFSTTDQVEKNLTTLIDFVQAPYFTEETVNKEKGIIGQEIQMYEDDPNWRMFFGILN
                     NLYPTHPLHIDIAGTVESIDKITAQDLYTCYRTFYQPSNMVLFVVGKMEPEKLMKLIR
                     ENQEAKNFPPKQEIVRYFPENTKEIIKQSALEAAITRDKFVLGIKGLDTLPQEGTELL
                     RYKTAINLLFQMILGNTSRNYLAMYNQGIIDDSFGFEFSLDREFHFADFSGDTDEPEK
                     AAEKVKEIILGFADDPEVSETNLDLLKKKMLGQYFQSLNSIEYIANQFTQSLFGDRTL
                     FDLPEIIDSIQMKDVLAAGEAFISEEAFSEFYMRPK"
     gene            complement(24697..25965)
                     /locus_tag="IXT20_000440"
     CDS             complement(24697..25965)
                     /locus_tag="IXT20_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HAQ4858917.1"
                     /translation="MVYQLAEGVNLHVLPTKQYKTIRIFIRFTARLQQEVITKRSLLS
                     SMLETNSLNYPDQTKLSAQLAELYGASFGLSVRKKGNLHWLNAGISFVNGEYVNDPNL
                     FSQAVDFLKEVLFYPNIKNHQFDQLTFDLEKNNLRLYLESLKEDKQTFASYALQELYF
                     ENSPEQKIPGLGVVEDLDKITASSLASYYQEMMANDQIDIFVVGDVDPDKAAEAVGQL
                     PFESRETVYPDLFYTQPQVNIVKERQVREPIVQAKLNLAYQTNVYYDEPERFALMVFN
                     GLFGGFPHSKLFMNVREKESLAYYASSSVDTFRGFMSVQTGIDEKNRNQVLHLIHEQL
                     ESLRNGEITELELSQTKAMLRNQYLLSLDSPQAAIEASFLDSWLPETKLSDEEWLKRM
                     ESVTIKEIQQVAEQIELQAIFFLAGGNANE"
     gene            26217..26678
                     /gene="mscL"
                     /locus_tag="IXT20_000441"
     CDS             26217..26678
                     /gene="mscL"
                     /locus_tag="IXT20_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HAQ4858918.1"
                     /translation="MIKEFKEFIMRGDVLDLAVGVVIGSAFTSIVNQVVQGLITPLIG
                     WFVAMITGTSDLEGALSILDWSPTKGVTFAFGNVISAIITFLITGFVLFLVVKAANRA
                     KNLRSKEEQVEVEETPTAEDYLSDIRDLLAKQVAEDNTHPVEQTNLSDTSK"
     gene            complement(26737..27183)
                     /locus_tag="IXT20_000442"
     CDS             complement(26737..27183)
                     /locus_tag="IXT20_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ4858919.1"
                     /translation="MEYQVKKFEELSTAEFYAIVKERIAVFVVEQQCPYQEVDDIDGQ
                     AWHTFFQDTDGKILAYTRVYEEAESIHFGRVLVHQDNRKDGLGRKIVQETINMIQKNF
                     PEKPIVIGAQEYLQAFYESFGFKAISDVYLEDDIPHIDMKKEQVAS"
     gene            complement(27276..27521)
                     /locus_tag="IXT20_000443"
     CDS             complement(27276..27521)
                     /locus_tag="IXT20_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4858920.1"
                     /translation="MPTTLASIKKDLEGRIGSPIMLVAQTGRKRQTERRGVLTETYPS
                     VFVVDLDPEENSFERVSYSYSDVLTRTVEIEFLTEAV"
     gene            complement(27636..28214)
                     /locus_tag="IXT20_000444"
     CDS             complement(27636..28214)
                     /locus_tag="IXT20_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAQ4858921.1"
                     /translation="MSEELLRAARNGDEEAFAALYQKYFPLLLRLKNTYYLRNYVSED
                     WEQEARIALYQALRTYEKSKGVSFGSYYKSIISNQIYSLLRKQNALKRRDQKDEVSID
                     QKIETEGPDFLAEITLQEPLAIQHLLLKEAIENCEIQFSKIEAKIFHYYMQGVDFVDM
                     AECLAMDHKQVMSAYGRVKLKIKKHLKFYNEE"
     gene            complement(28291..28821)
                     /locus_tag="IXT20_000445"
     CDS             complement(28291..28821)
                     /locus_tag="IXT20_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HAQ4858922.1"
                     /translation="MKKQLLIVDGYNMIGSWPELVQLKKQDKMADAREALLHRLSNYA
                     KYEGMEVIVVFDAQLVPGIQQNYKKYQLDVVFTKEDETADSYIERIAGQLNDRLTQVT
                     VATSDLAEQWMIFSQGALRTSAYELYKTIKKTEKTIAQENKDMHFIDFRRNSPWNEEQ
                     LKKLSQKLDDLMRKNK"
     gene            complement(28825..29685)
                     /gene="rlmB"
                     /locus_tag="IXT20_000446"
     CDS             complement(28825..29685)
                     /gene="rlmB"
                     /locus_tag="IXT20_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HAQ4858923.1"
                     /translation="MGKERTNKHQSKRGFEDKKQNRYSNKKQTGRNQSVSDQNEEALE
                     DNFVFGHHAAVEALQQGRGNKLFLQEDSRGEKVEELKEIAREQAVPVKWVPKQKLETL
                     SDHGVHQGVVLAITPYEYLSLDQLIERTKEKKEDPFFLILDSIEDPHNFGSILRTADA
                     SGVDGVIIPKHRAVGITPIVTKTSTGAVEHVPVARVTNLVQAVQQLKETGFWVFGTDM
                     TGTNFQKWNAKGSIALVIGNEGKGMSPGLKKEMDELLTIPMTGHVQSLNASVAAGLLM
                     YQAFSQRQGE"
     gene            complement(29678..30073)
                     /locus_tag="IXT20_000447"
     CDS             complement(29678..30073)
                     /locus_tag="IXT20_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HAQ4858924.1"
                     /translation="MRDYTQLNGLALAYVGDAIYEIYVRDHLIDQGETKPNKLHRAAT
                     NYVSANAQAFLIQQMLEEHLLTEEEELYYRRGRNAKSHTSAKNADVTTYRVATGFEAL
                     MGYLHLTKQTERMEELIRWCIQKVGEKNG"
     gene            complement(30070..31482)
                     /locus_tag="IXT20_000448"
     CDS             complement(30070..31482)
                     /locus_tag="IXT20_000448"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HAQ4858925.1"
                     /translation="MIKIYNTLTRKKEEFIPIQKGHVGMYVCGPTVYNYIHIGNARST
                     IAFDTIRRYFEYRGYKVDFVSNFTDVDDKIIRTANELGITAPEVADRFIRAFEEDTKA
                     LNVEPATLHPRVMDHMPDILAFIQVLVEKGYAYESSGDVYYRTRKFENYGKLSDQSID
                     ELEVGASQRTGEEQQQKEDPLDFALWKQAKPGEISWDSPWGKGRPGWHIECSVMATKH
                     LGETIDIHGGGQDLEFPHHENEIAQSEAKTGKKFANYWMHNGYVTIGEDDEKMSKSLG
                     NFITVHEMIKNVDPQVLRFFMATTQYRRPIRYSETTMKEAATNLQRLKNTFDNLKFRQ
                     ETASENLADDADKIALLENLEQRFIEEMDDDFNAANGITVVYELAKWLNTYLDQPKVS
                     AAVLAQGEALFSQWLSIFGILFTAEELLDEEIEQLIEERNQARSNRDFVRSDEIRDLL
                     KEKGILLEDTPQGTRWRREA"
     gene            complement(31479..32015)
                     /gene="cysE"
                     /locus_tag="IXT20_000449"
     CDS             complement(31479..32015)
                     /gene="cysE"
                     /locus_tag="IXT20_000449"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HAQ4858926.1"
                     /translation="MSWFTRAIDAAKRNDPAARSTAEIVLTYPGFHALFWHRFSHFLY
                     QHRLFLLAKINAQFWRFLTGVEIHPGATIGTGVFIDHGMGIVIGETAEIEDDVILFHG
                     VTLGGTGKETGKRHPTVKQGAMLSANAQILGPVTIGKNAKIGAGAVVLKDVPDDATAV
                     GVPAKVVRIKGEKVRRSE"
     gene            complement(32337..33797)
                     /locus_tag="IXT20_000450"
     CDS             complement(32337..33797)
                     /locus_tag="IXT20_000450"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HAQ4858927.1"
                     /translation="MTKVRVRYAPSPTGHLHIGNARTALFNYLFARHNDGDFIIRIED
                     TDQKRNIEDGEKSQLENLAWLGMNWDESPENPGEYGPYRQSERKEIYQPLIDQLLASN
                     RAYKCYCTEEELEAEREAQRARGEMPHYAGTCANLTPEEQAEKEAQGLESVVRFRVPR
                     NTEYAFTDMVKGAISFESDNIGGDFVIQKRDGMPTYNFAVAVDDHLMKITHVLRGDDH
                     IANTPKQLMIYEAFGWTPPTFGHMTLIINSETGKKLSKRDESILQFIEQYRELGYLPD
                     AMFNFIALLGWSPVGEDEIFEHQELIKMFDPKRLSKSPAAFDAKKLQWVNNHYIKAMD
                     LDAFTDMCLPYLIADGRVAENPDEKTIEWVKKIVSLYQPQMSYAAEIVDLSTLFFNEH
                     PVLNEAAKEVLAGETVPTVLHAFKAQLEQMEVVDAPTVKAAIKAVQKETGVKGKNLFM
                     PIRVAVSGQMHGPELPETIELLGREKSLAHLNQVLN"
     gene            complement(33967..35124)
                     /locus_tag="IXT20_000451"
     CDS             complement(33967..35124)
                     /locus_tag="IXT20_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN/TRAM domain-containing protein"
                     /protein_id="HAQ4858928.1"
                     /translation="MQKRVITLLMIIVGASLGISFLPSAWQAIGQQSNGWLNNTFTNS
                     LLGALIFFLLSLLLAKHISAIIKQVEQKLSEVSLTYLLFGAIGAIIGLTLGVVISTPL
                     YNMEIPFVNSVLPILLMIILGYLGLRMGTTRIEEWKKVFGSRSKKIEQETASQVESQL
                     LERKSGENFHKYKILDTSVIIDGRIYDITKTGFLEGTLMIPNFVLYELQYIADSGDSL
                     KRVRGRRGLDILNALQKEEGISVEMYDGDFEDISEVDSKLIKLAKLLDGVIVTNDYNL
                     NKVSEFQNVPVLNINALANAVKPVVIPGETMNVLVVKAGTERQQGVAYLDDGTMVVVE
                     DGQHYMNERIEVVVTSALQTAAGRMIFAKPAHAGRGIDNKKETEKKTKQSK"
     gene            complement(35158..36528)
                     /gene="radA"
                     /locus_tag="IXT20_000452"
     CDS             complement(35158..36528)
                     /gene="radA"
                     /locus_tag="IXT20_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HAQ4858929.1"
                     /translation="MAKKSKVQFVCQNCGYVSPKFLGRCPNCGKWNTMVEEIEQDTTD
                     RRTRTSLTGEKAKPTKIADVVPKKEPRIKTKLEELNRVLGGGVVPGSMVLIGGDPGIG
                     KSTLLLQVSQQLAAIGGKVLYVSGEESAEQIKLRAERLGSINTEFYLYAETDMNEISR
                     AIEHISPDYVIIDSIQTMTQPDITSVAGSVSQVRETTAELLKIAKTNGIAIFIVGHVT
                     KEGSIAGPRMLEHMVDTVLYFEGEQHHSFRILRAVKNRFGSTNEIGIFEMHEHGLEEV
                     ANPSQIFLEERLDGATGSAIVVAMEGTRPILVEIQALVTPTMFGNAKRTTTGLDFNRV
                     SLIMAVLEKRAGLLLQNQDAYLKAAGGVKLNEPAIDLAIAVSIASSYKEKGTQPTECF
                     IGEIGLTGEIRRVSHIEQRVKEVQKLGFTKVYLPKNNLGNWEAPKGIEIVGVATLAET
                     LKRVFR"
     gene            complement(36591..37118)
                     /locus_tag="IXT20_000453"
     CDS             complement(36591..37118)
                     /locus_tag="IXT20_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HAQ4858930.1"
                     /translation="MRKRGFEVISKYKDQNINLPQRATHHAAGYDFEAAEEVVIPSIW
                     KAGWKKGLETVADGLKKDVSENEKQLKPTLVPTGIKAYMGEDEFLQLANRSSNPLKRF
                     LVLTNGVGVIDSDYYNNEANEGHIMFQFTNFGFKDVVIKKGERIGQGIFLPFLKADQD
                     QTTAQRQGGFGSSSK"
     gene            37348..38154
                     /gene="proB"
                     /locus_tag="IXT20_000454"
     CDS             37348..38154
                     /gene="proB"
                     /locus_tag="IXT20_000454"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HAQ4858931.1"
                     /translation="MRKEIKNAKRIIVKVGTSTLIYPNGNINLAAIDQLAFVLSDLRN
                     RGKEVILVSSGAVGVGMHKLHLSERPTLIPEQQAAAAVGQAELMNIYNQRFSVYNQQT
                     AQILVTRDVVDYPESRKNVRNTLEQLLIMDTIPIVNENDTVAVDELDHLTKFGDNDQL
                     SAIMAKLVAADLLIVLSDIDGFYSDNPTTNPEAQMYRYIDKIDEKILAQAGGAGSTFG
                     TGGMQSKLKAASRIFEANSAMILANGKNPAIIFDILAGKEVGTLFKEDLK"
     gene            38151..39398
                     /locus_tag="IXT20_000455"
     CDS             38151..39398
                     /locus_tag="IXT20_000455"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HAQ4858932.1"
                     /translation="MTDLMQLGQQAKDSAQQLALMPTNRKNELLVQMAQTIKKNQQAI
                     IEANHKDLEKAVENNISETMQDRLQLTPERINAMATEIEKIASLDDPIGKVDEMWTNT
                     DGLRIGKKRVPLGVTGIIYESRPNVTTDAASLAFKSGNAVILRGGKEAFFSNQLLVQL
                     LQQVLVSAGESPYAIQFVDDTSHETAGQLMRLTEYLDVLIPRGGAKLIQRVKEQATVP
                     IIETGTGNNHVYIDKEAQLSMAVNIIVNAKASRPSVCNAAETLLIHSEIAPFFLPAIE
                     KELVEHGVSLRADARALEYLETAALAEDTDWDTEYLDYILAVKVVDSLDEAIAHINRH
                     NTKHSETIVTDSYAASQRFLNEVDAAAVYVNASTRFTDGSVFGFGAEIGISTQKLHAR
                     GPMGLNELTSTKYIIYGDGQIRE"
     gene            39474..39818
                     /locus_tag="IXT20_000456"
     CDS             39474..39818
                     /locus_tag="IXT20_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4858933.1"
                     /translation="MFEILMIFFIYLISLNIAAFLGVGILSLFFQFKKRSIGSQREKW
                     SQYFDKIGQKGLVTRLHISYMVALCLLATINYYSFFDHSIAYTITLLIAGIFHLSYKY
                     QLNKNHLNRTFR"
     gene            complement(39935..40261)
                     /locus_tag="IXT20_000457"
     CDS             complement(39935..40261)
                     /locus_tag="IXT20_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4858934.1"
                     /translation="MLEEYFQEVGLTLVELPIEFSDDRLIYRGYIKELPIINAEGETR
                     KAMQQKIAGMYQVYREQLLAESAEEEKEKTMNLSMDELLRYYDGETFDGFSLLNEEET
                     DLPGSW"
     gene            complement(40634..41835)
                     /locus_tag="IXT20_000458"
                     /pseudo
     CDS             complement(40634..41835)
                     /locus_tag="IXT20_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            41952..42467
                     /locus_tag="IXT20_000459"
     CDS             41952..42467
                     /locus_tag="IXT20_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAQ4858935.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            complement(42666..43112)
                     /locus_tag="IXT20_000460"
     CDS             complement(42666..43112)
                     /locus_tag="IXT20_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HAQ4858936.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(43159..44637)
                     /gene="msr"
                     /locus_tag="IXT20_000461"
     CDS             complement(43159..44637)
                     /gene="msr"
                     /locus_tag="IXT20_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Msr family ABC-F type ribosomal protection
                     protein"
                     /protein_id="HAQ4858937.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRPDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            complement(44991..45620)
                     /locus_tag="IXT20_000462"
     CDS             complement(44991..45620)
                     /locus_tag="IXT20_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HAQ4858938.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMQPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITTFE"
     gene            complement(45604..46068)
                     /locus_tag="IXT20_000463"
     CDS             complement(45604..46068)
                     /locus_tag="IXT20_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAQ4858939.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIEH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            complement(46555..47823)
                     /locus_tag="IXT20_000464"
     CDS             complement(46555..47823)
                     /locus_tag="IXT20_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-glycoprotein
                     endo-beta-N-acetylglucosaminidase"
                     /protein_id="HAQ4858940.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGSTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
     gene            complement(48054..48791)
                     /locus_tag="IXT20_000465"
     CDS             complement(48054..48791)
                     /locus_tag="IXT20_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAQ4858941.1"
                     /translation="MNDLSEKVIVIMGASSGIGEATTKLLAEKGAKLVIAARREDRLK
                     AIKESLPEAELYIQTADVRDFAQVQAVIDLAMEKFGRIDVLYNNAGIMPTAPLVEGHR
                     DEWQNMLDINIMGVLNGISAVLPIMEKQKSGHIISTDSVAGHVVYPDSAVYCGTKFAV
                     RAIMEGLRQEQRQNNIKSTIISPGAVQTELYQTISDKKAALELHEAQKEWGLTSEDIA
                     SAVAFAIETPDRMSVSNMIIRPTTQEV"
     gene            complement(48858..49427)
                     /locus_tag="IXT20_000466"
     CDS             complement(48858..49427)
                     /locus_tag="IXT20_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HAQ4858942.1"
                     /translation="MTSLIEKQIVNKEIRVTDPLFEVIHQIQAENEEPLAALNTGYHE
                     PADIRKRLENIISDKVDDTVTVLLPFYTDFGKHISIGKNVFINRQVMFVDLGGICLED
                     SVLIGPRVNLITVNHLTDPKERRGLSVKPIHIKKNAWIGAGATILPGVTIGENAIVAA
                     NATVTKDVPDNTIVAGIPAKIVKPVERII"
     gene            49555..50427
                     /locus_tag="IXT20_000467"
     CDS             49555..50427
                     /locus_tag="IXT20_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAQ4858943.1"
                     /translation="MDIRILTYFLAVAREKNISRAAKSLHITQPTLSKQLKDLEEELG
                     VTLFTRGSREIQLTDSGRYLYSKGKEILSLVEKTANNLTNDAIISGEIYIGGGETKAI
                     SFISDSLHSLIKAHPDIQFHLYSGNADDIIDKLDSGLLDFGLVIEPTNKQKYQSFRLP
                     AQDRWGLLIHRNHPLAKQTVIHPQELKTVPLIISRQTSVDSQLAEWLGFSIDQLSIVG
                     TYNLLYNASLMVDNGTFGALALDGIINTYGTEKIFIPLDPPLQASLNLIWKKDQPLSP
                     AARKFLDVLTEKYS"
     gene            50769..51641
                     /locus_tag="IXT20_000468"
     CDS             50769..51641
                     /locus_tag="IXT20_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ4858944.1"
                     /translation="MKIKKIFNQNAVLVDDGGQEKVAIGKGIGFDRKRNDLIFDRDVE
                     RIFVMEPEGQIKLQNLLNQIDERFLFAAETIIDHAETVLMEKLNEHVLIALTDHIAFS
                     AENINNGIVIRNKLLREIEVLYSDEFSIAQWAVEYLTKELGIPYTYDEAGYIAIHIHS
                     GRSGRNNNHRSIREVTIVSDIIHLVESELETDIHSEPFSLNYSRLVNHLRLLLQRTHA
                     QQYAVLDTEIVEMVKRKYPESYKISKKIRVLLTKEYQLAITKEELGYLAIHIERLRSA
                     IVQTNVNNHEEEKN"
     gene            51638..53596
                     /locus_tag="IXT20_000469"
     CDS             51638..53596
                     /locus_tag="IXT20_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ4858945.1"
                     /translation="MKAYLQRMGRSLQLPVAVLPAAALLVGIGNWWASYSNDIVAHFL
                     QAGGNAVLGQLPLLFAVGLALGMSVDKSGAAALAGLVAFEVPVNVLKTDSVATLLNIK
                     PESVDPSFAQISNVFIGIIAGLIAAALYNRFHEAKLPMALSFFSGKRLVPILAAFVML
                     LISAVLLLVWPPVYSALVSFAKFILSLGWGGAGLYGFFNRLLIPTGLHQAMNSVFWFD
                     VAGINDIGKFLASEGTKGVTGMYQAGFFPIMMFGLPAGAYAIYRNARPENKAKTASLM
                     LAAAFASFFTGVTEPLEFSFMFVAWPLYVLHAIFTGISLAVSAFFHWTAGFAFSAGFV
                     DYFLSLKNPIANQPLMLIVQGLVTAVIYYFGFNFAIKKFHLMTPGREEADLSDDDTAT
                     TNSDNKYAAQASKIYAALGGADNVTSIDNCTTRLRLQVKDTGLIDQNKIKATGVPGMK
                     IIDGKNAQVIVGTEVQFVADEMAKLHGGAAARPAQTNTVVKTETFAGETIEQDIYAIA
                     NGKLIPITEVSDDVFAEKMMGDGYAVLPENGEIFSPIAGTITNIFPTKHAVGIQTDAG
                     IEVLLHMGINTVDLKGEPFTLYVEEGQKVARGQLIALVDLAAIQSAGKNTDMVVVFTN
                     GDKVQSLEIEPARDVKANDKIGSVSNKA"
     gene            complement(53847..54698)
                     /locus_tag="IXT20_000470"
     CDS             complement(53847..54698)
                     /locus_tag="IXT20_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAQ4858946.1"
                     /translation="MHYLVTGATGGFGGYALEFLKKMVPQNEIYVLVRSEEKGEALKE
                     AGFNIRIGDYDDLDSMKEALKGIDRLLFVSGVPGNRQAEHKNVVKAAQAAGVSYIAYT
                     SFADADHSISVLAPDHQFTEKMIKDSGISHTFLRNNWYVENELPIIGQALKTGQFVYA
                     AGNGKTGWALKREYAEAAAKVLVESDSPEILELSGNLTDYEELAKALERATGKELEII
                     EASDAAFVENLKEAGFPQEAADMFLAIQHDIKKDQLAISSDDLEKVLGKPLTSLEAAL
                     KEILDQE"
     gene            54821..55288
                     /locus_tag="IXT20_000471"
     CDS             54821..55288
                     /locus_tag="IXT20_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HAQ4858947.1"
                     /translation="MKYSLQFSDAIHVLAYIEMFQDTRSLSSEMIASSIETNAANVRK
                     IMSQLKKSGLIFTKTGKPQPSLAKSPDQISLLDVYRSIEGNTQLIQVDPKTNPNCVVG
                     ANIQEALEESYARLQKKAEEEMAKITLDSLIHKIAVLEKEKRPENTGLVERFL"
     gene            complement(55551..56033)
                     /locus_tag="IXT20_000472"
     CDS             complement(55551..56033)
                     /locus_tag="IXT20_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAQ4858948.1"
                     /translation="MAKYRIEEKPDFTVTGIGVELKSSNTDFVGLAAEKQAFEQKVIG
                     NGTIDALKQAAENHYFFTVNEPYNGKMMYYVGVQTSQDVPNTTRIIPFPKGTYLVVSA
                     LAESPEELRNDLTNTAFGHVLREVEDYAYVGGPNAVVEMGVTNGKYMGEIWIPVVNKP
                     "
     gene            complement(56133..56996)
                     /locus_tag="IXT20_000473"
     CDS             complement(56133..56996)
                     /locus_tag="IXT20_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAQ4858949.1"
                     /translation="MLKDLNQLIDYIEEHLTEGLSYKIIAENTGISEYQLKRIFTFIA
                     GMSLMDYIKNRRLALANRDLLDGSRVTETAFRYGYETVEGFSRAFRDWSGYLPSEISR
                     YGIQKSFPRLTFFIDVRGGKSMEFRIEKKEQFYVAGVAKKVPVKFEGVNQEIQKLAQT
                     ITETQREEMHKLGDLYPNQVINVSYGFDGDRLEEKGSLMHMIGFATTKENPYADLEML
                     AIEEHTWAIFPNKGPFPQTLQDTWGKIYAEWLPSSDYGLVEAPEISFTKWGEDFSNVY
                     SEIWIAVKAKK"
     gene            57594..58442
                     /locus_tag="IXT20_000474"
     CDS             57594..58442
                     /locus_tag="IXT20_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4858950.1"
                     /translation="MNNWLEYFPENVLERGYSYHLHGFVRHLNYTSKYLSATVSGTED
                     YKVVITWDEKTNMTCDCLYAIEGKKCKHMAAVLFAYEERPIKKSNYSLSELSSLVSSA
                     SSSLVRELLTEILIEHPHFIERFKVKMPFHAINYSDKLTTIIHKYDHIIKKNKNRKTA
                     KFIMEMRKFIQEAVESLIQQNAYLPAFELINEVIATLETFYWEPEDERTLLLIEDCYY
                     LWKELLAEAPHAEKRQMFSWFVCQVDHTDASYSKRYSIKILKEDFREKEFSNQKKKID
                     KKLKKR"
     gene            complement(58613..58954)
                     /locus_tag="IXT20_000475"
     CDS             complement(58613..58954)
                     /locus_tag="IXT20_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylulose kinase"
                     /protein_id="HAQ4858951.1"
                     /translation="MVGLKRKELAGRRFTRIVSVGGGAKNLAWLQIQADIFDATVYCL
                     SMEEGPALGATMIAALGLGWFDTPESCCQNFIQYTDAAEPLLQNVEKYHKIYQQCRKI
                     YPATKEICHDL"
     gene            59040..59747
                     /locus_tag="IXT20_000476"
     CDS             59040..59747
                     /locus_tag="IXT20_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HAQ4858952.1"
                     /translation="MDEKLDIKTAIKDTLPTVFGYIGIGLAFGIVGKAAGFHPLVVTL
                     MSLLVYAGSAQFITVSMLASHSPLLSIVFSTFLVNSRMILMSMTIAPYFKKNRLLQNL
                     LIGTLLTDESFALGMNKLNYTGQKLNFRWMNTANWISYLTWVGSSLVGALLGNFITDP
                     KKFGLDFAIVAMFIGLLYLQVISDRNTSKRLQLILIGLTLILVYVGLIFIPSNLVIVV
                     VTLIGCGLGVWIKHAFF"
     gene            59734..60057
                     /locus_tag="IXT20_000477"
     CDS             59734..60057
                     /locus_tag="IXT20_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HAQ4858953.1"
                     /translation="MPSFEFTLLTILGCTLATWLSRVLPFVLLKKFDLPQPLLEYLSF
                     VPIVIMSALWFSSLFTQNIGHLPQINLENALASVPTLLAAILSKSLLVIVLAGILSLS
                     LIRLL"
     gene            complement(60146..61183)
                     /locus_tag="IXT20_000478"
     CDS             complement(60146..61183)
                     /locus_tag="IXT20_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HAQ4858954.1"
                     /translation="MSEKELMKVVGFYEGLPIDDPKSFIDEKEQIPVPSSRDLLVKVN
                     AVSVNPVDTKLRQDNGIRNALRILGFDGVGKVVAVGDEVQKFSVGDRVFYAGTTTRAG
                     SNQEYQLVDERIVALAPKNLSDEEAAALPLTSLTAYELLFEKFGLTPEENANRGKKIL
                     VINGSGGVGSILNQLAHWAGLEVYATASPKNFEWLKKTGVDYPIDYHHDLSKSFRELG
                     IDQVEYVAVLFDITRYFDQIKHLVRPFGHVGTIVGIKEPLDIGVWKNQSISFDWEYMF
                     AKTDYNYEIETQGEALAHIAALANEGIIRSTLSKVYSDGMNAKNLKQATKDVETGHML
                     GKVVVSGPFNG"
     gene            complement(61630..62613)
                     /locus_tag="IXT20_000479"
     CDS             complement(61630..62613)
                     /locus_tag="IXT20_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HAQ4858955.1"
                     /translation="MTTNFWKELPKPFFVLAPMEDVTDVVFRHVVKHAAAPDVFFTEF
                     TNSDSFCHPDGKDSVRGRLTFTEDEQPMVAHIWGDKPEFFREMSIAMAEMGFQGIDIN
                     MGCPVPNVAERGKGSGLILRPEVAAQLIEAAKAGGLPVSVKTRIGYTEMSEMEEWISH
                     LLHQDIANLSVHLRTRKEMSKVDAHWEVIPKIIALRDQIAPQTLITINGDIPDRQTGE
                     ELAEKYGVDGIMIGRGIFKNPYAFEKDPREHTEKELIGLLRLQLDLQDHYSEIIPRSI
                     TGLHRFFKIYIKGFPGANDLRVQLMNTKSTDEVREILETFEKEHPTLFSEQ"
     gene            complement(63029..63799)
                     /locus_tag="IXT20_000480"
     CDS             complement(63029..63799)
                     /locus_tag="IXT20_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ4858956.1"
                     /translation="MFNLYFTALKSLAIKETNRYLRIWVQTLVPPVITTSLYFVIFGK
                     MIGGRIGDMGGFSYMEFIVPGLIMMSAITSSYANVSSSFFSQKFQKNIEEILVAPVPT
                     HVIIWGFVIGGVGRSILVGSLVTIISLFFVPLHVYSWFMVIITLLMTAILFSLAGLLN
                     GIFAQSYDDVSIVPTFVLQPLTYLGGVFYAISMLPPFWQAVSKVNPIVYMISGFRYGF
                     LGTIDVPVATSMIVLVLFIVVLYAVTWYLINKGRGLRS"
     gene            complement(63792..64727)
                     /locus_tag="IXT20_000481"
     CDS             complement(63792..64727)
                     /locus_tag="IXT20_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ4858957.1"
                     /translation="MANALEINNLKKIYGSGVEALRGIDLSVKEGDFYALLGPNGAGK
                     STTIGIITSLVNKTSGKVNVFGYDLDKDLVRAKQQIGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVSRKEAMKRSEKYLKQSNLWEKRNVRARMLSGGMKRRLMIARALMHEPRLLIL
                     DEPTAGVDIELRREMWEFLRELNENGTTIILTTHYLEEAEMLCRNIGIIQSGELIENT
                     SMKELLSKLQFETFIFDLAPYDTKPVIEGYACTFEDEQTIAVEVERNQGVNGIFDQLT
                     RQGIKVLSMRNKSNRLEELFLKITEENNHTEEKNV"
     gene            complement(65321..66970)
                     /locus_tag="IXT20_000482"
     CDS             complement(65321..66970)
                     /locus_tag="IXT20_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HAQ4858958.1"
                     /translation="MGQREFIVLIIIAVVILLVVLDIFSRLKVKETVRSNWGKIPYQP
                     RFDKEESLKDAWLTEKKFRSWDSEIDDLTWYDLDMFEVFEGINSTYSSVGSEALYQRL
                     RSFDFGEDYQLEKLIAFYQENPQLRERIQYQFARLGKKDHNFAKQYLADGKSKKIGRV
                     GLFIFLGLLPLVGVGLLLLGQVLGIYLAIGGLVFNTVYYLVKKQTLETELNSMSYLVS
                     TISAANQLAKMTTPLQKELKQQTKPLRDIPKLGFSFRMKSGSEAELIFEYVNIMFMLP
                     FISYHFVLTKLEKHSTEAIKLWELLGELEVAAAVLNYRTYMPITCRPEFTEGGVTAKD
                     IYHPLLKEAVVNPVNWQKNTLVTGSNASGKSTYVKSIAISCILAQTIQTAIAEQFTLQ
                     PGHVLTSMAIEDDLFEGDSYFVSEIKSIKRLLDQVASKERCYCFVDEILKGTNTIERI
                     ASSASVVRWLAEYPSLAFVATHDIELTEILKNYCENVHFEEQVTEGKGISFDFKLKEG
                     PSRTRNAIALLKVLNYPKSIVEQAQKESLLFDEQRQWYPFD"
     gene            complement(67165..68061)
                     /locus_tag="IXT20_000483"
     CDS             complement(67165..68061)
                     /locus_tag="IXT20_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAQ4858959.1"
                     /translation="MIKWLLLLVVIFMGSFIFFLQREIKRLTKKIATLSERQEYGGRL
                     SVEFREKNLMDLVDALNTWVDENEQKGQAFQEMEENIRLSIVGLSHDLRTPLTAINGY
                     VQLLEQVEDTEKREKYLTIIKQSVARLLSMTDQFYDLARIETKQKEMDLHTLSFSKEV
                     EEVFFSFYEQFSEKNIEVAFAEHSINTTVLADPLMLLRVLQNIIQNLLRYADGQAKVT
                     FSKEDSFIRLTVANPIKPGAQTSVEKVFQRFYTENTSRTNTESSGIGLYLSKKLVEGM
                     GGEMTARLDGGIFSISVKLRRV"
     gene            complement(68054..68734)
                     /locus_tag="IXT20_000484"
     CDS             complement(68054..68734)
                     /locus_tag="IXT20_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ4858960.1"
                     /translation="MFLLETILIIEDDEAVHSLLEEVLEQRYKLLDAYSGTEGKLLLS
                     TYPVDLILLDLMLPGLSGEALLAEIRQTSNVPIIVLSAKSDQRDKVSLLAAGADDYVT
                     KPFDIEELLLRIGIQLRHQTSVQVKDLSQRIYYKEILLDKESRDVNVNGVAVHLTGRE
                     FDLLKLFLEHPKKVFSRANLYETVWQEPYFDSEKTVNMHISNLRGKLAKAHAVYIHTV
                     WGVGFRFD"
     gene            complement(68735..69517)
                     /locus_tag="IXT20_000485"
     CDS             complement(68735..69517)
                     /locus_tag="IXT20_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAQ4858961.1"
                     /translation="MFNLLTAEKIKVWKSRKMWVSLGLMVVLPLFYSWNEWYMHTKYG
                     NELNQATDTVINGATGILMVEKMAAWILLAFAAFACFYIGEEFQNGTIRNTLSLGRNR
                     MTYYVSKLLVTLLITTLGVVLITGLAMIAYTLAFGFGEVEGIKNYGNYVLKVFPVLLL
                     LILATLSVPVTLTFITRSTSVSLLLSFLYIMGTAFVPGVFAKIKGLEFLTEWFTETWL
                     MYTDFAQQATYSQAPKMILVSLVTIVVSSALGMFIFQKSDIK"
     gene            complement(69510..70427)
                     /locus_tag="IXT20_000486"
     CDS             complement(69510..70427)
                     /locus_tag="IXT20_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ4858962.1"
                     /translation="MDFGNETVLKATGITKKYGAAKALDKVSIEIKRGMIYGLIGENG
                     AGKSTFMRTIMGLISIDEGSIELFGTTDLQAARRRMGQSIETPALYPELTARDNLRIQ
                     AANGGVSDREIEDLLKMMRLENTGKKKAKNFSLGMRQRLAIANALITNPEFLILDEPT
                     NGMDPAGMAEMREIIQRLVKERGITVLLSSHLLDELSQIATHYGILHEGHLIKELSKE
                     ELAQESRQFIKIDTSATEQAVTVLDSLGYRDYFVQSSRVIQLFEGIDQVAAINQALVE
                     AKVPVDGIHLVGQKLEDYFLQLTGGNPHV"
     gene            complement(70430..70714)
                     /locus_tag="IXT20_000487"
     CDS             complement(70430..70714)
                     /locus_tag="IXT20_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ4858963.1"
                     /translation="MFSFAFYMVWLQREVPTHIQGRLMSTITTITMVASPLGLALFGA
                     LFDWQTTQPRLRDSLIFLEIQLENLHFLYTFLAAFFIPVNYTSLINKKEG"
     gene            complement(71415..72035)
                     /locus_tag="IXT20_000488"
     CDS             complement(71415..72035)
                     /locus_tag="IXT20_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4858964.1"
                     /translation="MNINRVIKLIGAKNINKVRSEFIFPEDFFNWFFYQFSECKRLLG
                     ENYYLKGIGGFMGNVADEQNIIKGISEQTSELTVTKAFISNGESLKNVTARIRKENEV
                     DIVFGIDHECNSVIYVTQSANLILFENGCKDDFMVVYLYDILIPKLRSLYMSSESIFV
                     SEQKGSFSKKIGIEVIESIMCHNHITLEELEILIGQKNNNTKIQVS"
     gene            complement(72116..>72557)
                     /locus_tag="IXT20_000489"
     CDS             complement(72116..>72557)
                     /locus_tag="IXT20_000489"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4858965.1"
                     /translation="YGSPLFCECVVVTLFYRRDFLFSIYTKYFTLSSNIKTSTKLICK
                     LFKLDKISNIDDVKSKILTRKVSEPVKLTAQDKKNGIVLYGDDAKYNYNWDTSSEAEV
                     LSIPTTDTSGEKVTFKINFITAISKLEYGKQKKRDNNDQYLPKK"
BASE COUNT        20875 a        15460 c        12775 g        23447 t
ORIGIN      
        1 ggtactgttc tatggggcgc ttacttaata ccgagaaaat ggtttttgaa catcacttat
       61 tcaaaataaa aaaagattgt gacacgaatt ttgtcacaac ctttaaatgt attcctagag
      121 caaagaagct aagcgaagaa atccaattct gattatttat tgtatttctt tgttcagcat
      181 ccttgctaaa agcccatatt ggacaaagta ttcactacac ccatgatctc atcttttcga
      241 gaacgtgctt tttctgtcgg atcttctacc tcttctgtgt caattcgaac aataaatttc
      301 cgattcatcg tcacgtaagc accgtcgttg atcaaaataa acagttcgtt ctcattatag
      361 tctgcacagg catctttcca tacgtcaaag ccgttttttt cagaagggat atcccgttta
      421 accacagttt ttccacttaa ttcgtggaac gttacgtcat aatatttcat tatctctacc
      481 tctttcgtca ttcaatattt tgtacttgtt ctctgatttt ttctaagatc gtcttagact
      541 ggacgacttg atttttaatt tcgattgcat ttgatttgga accgatcgtg ttgatttccc
      601 gattcatttc ttggatcagg aaatctaatt cccgacctac aggacattgt gcttcgataa
      661 gttcatctag tttttgtaca tggatcgcca gacgatccag ttcctcgtga atatctccgc
      721 gttccaacaa gatggctagc tccgtcaaca accgttgctc atcgactttc gctccaagaa
      781 attcctccag ctttttttga taccttattt gaaattcctt ttcatagatt tctacaaaac
      841 tatcgagttt ttttagtact tctttgaatt cttcagtatt ctttttcaac actaaagcta
      901 aagcttgacc ttctttttct cgactcgcct caatagaatc aagtgcgctt ttcaatgtat
      961 ctagagctaa ctgtgccaat tcttccatgc tctcttccgg tttttcttct accgttacga
     1021 agttttcgtc cagcgcgaag cgttccgcca tccgactaat tgacacttga ttttgactac
     1081 cgtatcgttg ttgaattccg tctgtcaatt gagtgataag tgaatcaatc aattcccagt
     1141 tgatgacgat ctgtttttcg tttttgccaa tataagctag attcacaaat acttcgattc
     1201 gaccgcggct taactgctgt ttaataagct gacgaaaatc gttttcaaca aagttcagga
     1261 ttttaggact tcgtatttga acatctagaa aacgctggtt gacacttttt atttcaatat
     1321 ctaactgata cgcttttgtt tcgcggatgg ctttgccaaa accagtcata cttttcattc
     1381 gacagggtcc ttcctcatta catattttct attgcttttt gcagttgttc aaactcttcg
     1441 ttcgtcaatg tcaggccttt ccccattttt tcatggtctg gcgcccaatc tcgaagatcg
     1501 aattttggcg ggcgaccatt ccagctgact aggttcagtt ctttacgcca ccccttgttg
     1561 ttttctgaca atatagcgat ttcttctacg atttcatatg aaaattcttg tgccatggtt
     1621 gttcctcctt gagataatat aaatatagga tcagtaatgt gtcaactggt tgcttaagca
     1681 attcatgctg caaacgatcc ttgcgttttc cttgtagcag ataatcaaaa taaacagttt
     1741 tcgtgcgctc attttgtatt ttttgctggt attttcgata ccttcgccat tcttcagtac
     1801 ttaattgacc aatttgtgcc aataaatcat ccggcatact ttttatatct tgttggcaga
     1861 tcatttgaat catcatgtct acttgataga tttcattggg cagtaaaaaa tgtttttttg
     1921 ctaaaagttc tagcatcgcc aatccgcatg tctctggtac gaccctgttc ttatataaat
     1981 tacgcagttt ttgccaaaat tttatgcttt tgaaaaaaga taattctaac tgaatttctt
     2041 ttgttaagta agacaaaaat cctcgccagt atgttcttgc aggtttaaca gaatgaaatg
     2101 tataccagtt cccatagtaa tagcctaatc gttttttcat ttccagatag cctaattgta
     2161 aatttaattg gttcctttgg ctatcaaaaa gcagaaagct gcctagtgtc cacaatgttt
     2221 gacatttcca atggataaaa atgtctggca tacgaatttt tttcgctggg cctggccctt
     2281 gcacatctac gacaaatgcc tctgttgctc ctttgttaat cgcaatatca ataggtattt
     2341 tgttgcgata cccaccgtct gcatatttat tttttccgat ctttcgataa gccatagcgg
     2401 gataaaatga agcagaggcg agtatccaat ctggtatttc ttctgttttt gtctgttgga
     2461 tatcagtcac tacttccgct agttctggca gtttagtaga caccgtatat aaacgtattt
     2521 tgggattatg tttgagtaag gacaaatcca aattgtcttt gaccatcttt tccaatggag
     2581 cagaagaaac cccgccttca attagtgctg ttttcgtcat ttggcgtctt tctcgataga
     2641 agcgttcacg aaggtcttca actgcagcca tttctggtaa cgcaagcact tgtcttgtcg
     2701 acagcttttt ccataaattc acagcttttt tcatatctcc ttgtaaaatc aatgcgccgt
     2761 tcaacgcacc taccgaggta cctgtgataa tctcaaagtt gatatcatgt tccttcagcg
     2821 cctgccacac accaatctga taagcaccgt gcgctcctcc tccacctaaa acaagtgctg
     2881 tatgatagac cagcttttta tttaattgtg tggttccttg atagccgttt ttatgaaaaa
     2941 tacgcaacca ttcttctgat agaggagatt gaaataaaaa acaacagttt tctttaataa
     3001 aaaaacgagc gcaagcttcc atataccgaa aaaatttttt ccattcaaaa gtttcacctt
     3061 caatcaacag attattaaaa ataacatctc ctctactttt agcaatagta aaatagacgg
     3121 tatatttttt cgatactgct ttaaaagcac attgcttttt tgcagaacgc caaaaagcgt
     3181 ctctactttc ttgatgactt tgaaaaaatg gaaggggttg ttttgcttgc tggataggcg
     3241 aatacagcga cattttctgt gaaaagtaaa ctttttttat ctccatcgcg ctctcctttt
     3301 tcttcaagta taccagtacg tcataaaaag gtcatgagtc tcgtattcga ctttgtagat
     3361 tccataagaa aacaattcat aaaacctcct ttaaataaaa aaatacaaaa atcacaaaaa
     3421 ttaattgaaa atgagagtgt tttcaatgaa aacgtgattt tgtaatggaa ttttaaaata
     3481 aaagaaacaa gcttttttaa tgcaaatgtt gtaaaataaa agagattaag aggaaataag
     3541 aggaggaaca tgatgactga gtcagtttat gtacatattg ataccacaag taatgctgtt
     3601 ttaacaaaag ggctgactgc tactgacttt gcaaacagta tcgtgcattt tcctcaaaac
     3661 ttgctcttgc ttgatccatc tgcttctgct ggagaatatg aatcccatac agggctgaaa
     3721 gtaatccgtg gtacagaaaa tattcagcgt ttcttttcct cttctcgtaa caggcgtgga
     3781 ttcgatgaat tgaagtggat cgactttact gatttaacga tgctgaaaga attgacacct
     3841 ttagaaattt cagaattatt atattttggt catatgaaaa cacatcttca ttcgccgttt
     3901 ttttataaac tacagaataa ttttgtatac ttcgatctta atgatcagct gaatcggatt
     3961 tattatcgct atttagatga attttatagg atttttgcta gtaaattaag ttcgatcttg
     4021 tcagataaga tcaatgtaaa aaaatctttt ttcaaaaaag aaacacctgt tgaaaaaatc
     4081 agtgtggaat tattgaaaga aatgcgtaat ttgatgcagg aagggatcat tttttctttt
     4141 gatcaagttg gtttgatcga tggtaaatat cgtatcccaa tctatgtgat tgaagattat
     4201 atttggaaat taaaaggcac ccggtatcga gaagaggaaa tcatcgctac tttaatctac
     4261 tctgtcaccg atcgacactg gcaattggta gaagatgccg atctttctta tctgccattg
     4321 aagtagatct atttcttatc aaaaaataaa aaggaaacaa gaaactagga catgattctg
     4381 tctcaagttt cttgcttctt ttttatttac agctcctgtc gtttatctgg cacaaaaatc
     4441 gaaggagtcg tttgttggat ccctaataaa acgatgatcg ttacaatagc tgtactcaac
     4501 ccgctggcac cattcattac gaaagaaaag agccaaggat tcatccccca tagtgcatac
     4561 gctccccaga aaaccactcc tgcaataaaa tgccaaaaat atctggaaag tgcgccaatc
     4621 aatgcaccaa acacgatgct ttttctaatt tgtttcgtat tatcgtctac atagcctttt
     4681 tggacatttg aagccatgag ccctgctgcg cctgcaaaag tataggcaat cgggtactca
     4741 atcaacactt gaacgacact taaataataa acttgtccca acggaaaatg taggatgccc
     4801 caaatgaaac ctgccagcat ccctgccttt gtccctcttc ttaacgcaaa aagagtcaat
     4861 ggaatctgcc ctaatgaaat agaaaaactg ctgccgatct ggatcggaat caacgacaac
     4921 accatcgcta acgctgcaac aatcgttcct tctacccaaa ctctattttt tcccataaaa
     4981 aaacttcctc ctttgaataa aaaagtcaca aaggaggaag cagctttcga aaatagtcaa
     5041 acagctgttt attcacaatt cctacgctcg cataaccgat acaggtacga agggtttaga
     5101 aatagattca atctcagccc aattggactc ccctttgtga gtttatttat tatttatttt
     5161 ttgtttcaaa caatggacta actggtttgt tttcatggat acgtttgatt gcatctccca
     5221 ttagtccgcc aacacttact tcatcgattt tgtcgatttt acgatcttct ggaagataga
     5281 tcgagtctgt aacaactaga cgctcgatag ctgaatcttc tattctttgt aaagcagggc
     5341 cggataatac tggatgtgta catgaagcat acacactagt tgcaccagct tctttcaaag
     5401 cattggcagc caatgtgatc gtacctgctg tatcgatcat atcatcgatc agtacacaag
     5461 ttttgccttt gacttgtcca atgatattca ttacttctgc aacatttgct tttggacggc
     5521 gtttgtctat gatcgcaatc ggagatttca aaaattccgc taatttacgt gcccgtgtga
     5581 caccaccatg gtctggtgaa acaacgacaa catcatcgcc tttgattcct ttttctaaga
     5641 aataattggc aataagtggc gcacccatca aatgatcgac tggaatatca aagaaacctt
     5701 ggatttgaac tgcatgaagg tccaaagtca gcattcttgt tgcaccagct ttttgaatca
     5761 tgtttgctac taatttcgct gtgattggtt cccgtgcacg tgctttacgg tcttgacgcg
     5821 catagccata ataaggcatg actacgttga ttgtttttgc acttgctcgt ttcagtgcgt
     5881 caatcatgat cagcaactcc atcaagttat cattgactgg gctgctcgtt gattgaataa
     5941 catagacatg cgatccacga atactttctt caatgtttac ttgaatttct ccatcactaa
     6001 attgagtaac agatgatttt cctaactcga cgccgactgc tgctgcaatt ttctctgcta
     6061 atgggcgatt ggaatttaac gcaaaaattt ttaatcttgg gtcaaagtaa tgttttgaca
     6121 tggggacctc cactttcttt ttcacaactc tagaattcta cctaataaat agtagtcata
     6181 ttttttaaat aaatcaagtg attttgcaaa agtcttagtt tagataaggg agtttttttg
     6241 catatccttc tttattgact tgtcttgctc tcgcaattgc cagcgcatat tctggaatat
     6301 tgtctgtaat tgttgacccg gcagcaatcg aactgttttt agctacctcg acaggtccga
     6361 tgatattcgt gctagaacca ataaagctgt ggtcgccgac agtcgtatga tgtttgttct
     6421 ttccatcata gttgacgaag acaacaccac atccgacatt gatttcttct cccagcgtag
     6481 catcaccaac ataagtcaag tgaccgactt tagttccttt gccgatttga gcatttttca
     6541 cttccacaaa gtttccgata tggacgtttt cgccaatttc agcttttggt cgtaaatgtg
     6601 cataaggtcc cacatctgca tgtttcttga catggctgct ttcaatgact gagttttcaa
     6661 tgacgacgtg atcctcaatc ctactgtcta cgatttttga atgtgacccg atgacacaat
     6721 cagaaccaat gactgtattt ccttggattt gtacacctgc ttctatcaat gtgtctggac
     6781 cgatttctac gcctgcatcg atgtatgtcg ttgctgaatc gacaaagctt acgccattga
     6841 ccatatgcat ctggttaatt cgctttctca tgatctcatt tgctttagct aacgcgatcc
     6901 gatcattgac tcccattgat tcttcaaaat catccgtttg ataagcagca actgtatggc
     6961 cttcttcttt taagatttcg atgatatctg ttagatagta ttcaccttga gcattgtttg
     7021 ttccaacttt gcttaacgca tcgaacaaag cttcgttgtc aaaacagtaa gttcctgtat
     7081 tgatttcttg tacaagagct tcttctggtg ttgcatcttt ttgttccacg attttttcta
     7141 cgatcccaat atggtcacgg atgattcgtc catatcctgt tgggttttct gcttgtgccg
     7201 tcaagatcgt tgcacttgca ttttttcctt ggtgatattc aaaaagattg ttcagcgttt
     7261 cagtagtcaa taatggtgta tcgccactga taactaacgt tgttcctttt tttccttcca
     7321 agaaagaagc agcttgcaaa acagcatgtc ctgttcccaa ttgttctgct tgtaacgcat
     7381 atttactgcg ttctcctagt tgtgctttaa cttgttctgc tccgtgtcca acgatcgtaa
     7441 tcacttcatc tggtttcgtt tcactgaccc gattaatgat atgttctacc ataggtttac
     7501 cagaaacggg atgtaaaact ttatataatt ttgacttcat gcgggtacct ttccctgccg
     7561 ccaaaataat tgcataacgt gcgtccaact gcgtcactcc taaatatagt ttgctgattt
     7621 cagtttatcc cagtataccc attttttcaa caaaacaagt gattttcttt tatcccagcg
     7681 ttttctggat aaaaaaatag atagaccata acgggcctat ctatttggta accggtgctc
     7741 tcctgccaac tcctcggtca caagtcacac tactcataaa acatgaaaag ctgttttttg
     7801 aaatcgtttc ttgtgactta gagttaaacg gtaggcttac aacctcttca ttaaacgttt
     7861 ttcaatcagc tgaccttcgg tattagctca ataaacggca aaatatgagg agcaattttt
     7921 ccgtttattg agctaatact caggacagga cgctgatttc acaacctcta gataaacggt
     7981 gttcaaaagc cagtttctcg gtattaagcc aaatttatga aaaaatgtgg aaagccattt
     8041 tcacaaaatt tttcttacta ctcgaaacta agcagctttt tcacaacctc ttattctgaa
     8101 aaataatttc ctggcacaac cgtaatattt ttcgtttgag tatctacatc tttgacataa
     8161 agcagtgacg tataatcatc gatggcgcga tgaccgttga attttgattc ggcaaataca
     8221 gtgatgccaa caagttctga ttcgaattct tcaatcaagc tcttcattcc gttgactgtt
     8281 ccgccgcctt tcatgaaatc atccactaca aggacacgtg aaccacgttt caaacttctc
     8341 ttagacagtt ccatcttttc aattctttca gaagatccag acacatagtt gacacttact
     8401 gttgatcctt ctgttatttt tgagtcccgg cgtacaataa cgaatggcgc attcagataa
     8461 taggacacag cttgggcaat aggtacgcct tttgtggcta cagtcattac agcatcgatt
     8521 ttttcaccaa ggtatttaga agcaatgatt cttccaactt gacgcagcag ttcaggttct
     8581 cctaacaaat cagagagata aacatatcct cctggaagca gacggtcttg ttctgataga
     8641 cgttctgcta gagattcaat atattttttc gcctcttcat atgaaatttc agggatgaac
     8701 aatacaccac ctgcagcacc cgggattgtt tccaaggttc cataccctcg ttctttaaat
     8761 gttttcttga tgattcccaa atcttcactg atagaagatt tagcagattc ataacgttcg
     8821 gcaaagcttg ttaaggaaat caattcatgc gggtgattca ataaatactg tgtcatgtca
     8881 actagacgtt cactgcgacg tactttcacc tattccaact ccttttaaga aattttatcg
     8941 ttttacatta tataagtttt tcttcaaaaa ttcgagcatt ttatgaaaaa ataagaaaat
     9001 agttcggtat ttattaaaat aaaaagaagc ctataaaaaa tttttcgttt ttataaggct
     9061 tcttttcttt ataatttagt tattttttct tctgaaggca accaatattc gtttgatgat
     9121 atttacaatc aggaacaaag caataaagac taatgtgatt gttgctcctg gaggtgtatc
     9181 tagataaaac gagctagtta gtcctgacag catccctaga aatcctacac aaacactgat
     9241 agcaatcacg acattgaagc ttttgcctaa tcgcataccg attgctgcgg gaagaaccat
     9301 gattgcggat atcaaaagtg caccggcaat cgggatcatc acagcaatag ccacaccagt
     9361 aatcacattg aacatcatcg acatcaaatg aacgggtagc ccatccacgt gggcggtatc
     9421 ttcatcaaaa gtcagaacat acatcggccg tttgaataaa aagaacaata taccgatggc
     9481 aacgaataag attcctaaaa agatgacttg ctcccatgta atggtcacga tcgaaccaaa
     9541 cagataggat tggatacttg ttgctgaatt ccctccgctc aaattcatca atactaacgc
     9601 tagcgccaaa ccaccagcca ttaaaatggc aatggagatt tctgaatagg ttcggtagat
     9661 cgttcgcaga tactccaata tcacagctgc taatacaacg actagcatgg tcgtaagtgt
     9721 aggattaagg ttcaaaaaga atcctaacgc tacccctgct aaagagacat gagaaagcgt
     9781 atcagccatc agcgattgtc ttctgatcac taaaaacaca cctagcatcg gggcgattcc
     9841 ggcaatgaag atagcggcaa gaaacgctct tctcatgaat tcatatgaaa gcaacgccat
     9901 tgagaatcct cctttctcac cagtcggatc tgtctgtccg catactgttt gatatcttca
     9961 tgatcatggg tgatcattag aactgatttc ccatggacat gcgcactatg tcggagaaga
    10021 cgataaaatt catttctgga ttcttcatcc atacctgttg ttggttcatc aagaacaaat
    10081 aagtcagggt cagtagcaaa aatacgcgct aaactgattc gctgcttttg tccgccagac
    10141 aattcaccta ttcgccgatt tcgcatatcc cacatcccta cagcttctaa agcacgctgt
    10201 acatgttgat gatctttttc agagagacgc ttgaaccagc ggcctcttgg atatcttcct
    10261 gaacggacta attcgatcac cgtactagga aaacctgcat tgaaagatgc aacttgttga
    10321 ggaatataac cgatacttaa tttttttcct tcttgatttt tctcagcaat ctttatcatt
    10381 ccttttgaag gtttcagtaa tcctaacgat gctttcacca aggttgactt ggctgctcca
    10441 ttttctccag ttaaaatgac aaattcaccc gcatctacat gataagagac atcttctagt
    10501 acaggttcat catcatagta aaaggtcaaa tcagatattt cgatataacg caccttacaa
    10561 cctccattct aaaacttaat tgatactttc ttttaaagct ttcagatttt tttccatgac
    10621 agaaatataa ttttcgcctg ctttgatttg ttcattagtc aagctttcta aaggattcaa
    10681 cacttctagc ttcactcctg tttcattcga caatgtctca gctaccttcg aagaggcatt
    10741 ttcttcaaaa taaatcactt tgatgttgtt tttcttcaca aattctttta gctcagctaa
    10801 gcgacttggt gtgggttcct gatctggtga aacgcccgaa atcgctactt gttttaagtc
    10861 gtattgattc gctaaataag caaaagcagc atgctgagtg acaaatgtgc ggtttgttgc
    10921 atcttttagc tcatcggagt atttttgatc cagtgcttca agctttttga tataagcatc
    10981 ggcattcttc gtaaaagttt cctttttatc tggatatttt tcgcctaatt cttcagcaat
    11041 cgttctgact tcttttatag ctaataccgg atcgagccaa acatgtggat cgtattcatg
    11101 tgaatgacca tgcccttctt ctgattcttc atgttcgtct gcttcttgcg attctaaacg
    11161 gtcgattcct ttagctgctt cgatcatcaa ggtctgtttg ctatccactg aatcctttaa
    11221 cgaatccaca aaaaattcca tgtctgaact attatacacg aaaacatctg catcggaaat
    11281 ttctgctctt tcttttgcac ttggttcgaa atcatgcggc tccgttccag cagggatcaa
    11341 aagtttcacg tttccttcat cccctacgat ctcttttgta aaatcataca tcggataaaa
    11401 agtcgtaacg atcgtcaaat cctctttttt gtctgcttcc ttgttcgtat ttccacatgc
    11461 tgcaaacagt aaagcaccta tcattaccgt agctgtcaaa agatattttt tcatttatat
    11521 gctcctttca atgaacaaat cggaatttta cgatttatta acgtgaacta atgtatcaaa
    11581 ataaaaaaac acagtcaata gaaatccatc aatttctgat ttaagcatgt atattcttga
    11641 ttaaatcgta aaatggatga gttgctgctc atatctagcc atttcactac aataaaaaac
    11701 tgaagaatac cattttagga ttcctcagtt tttcatcacc tattttactt cttatatttt
    11761 attgcagggt tcgtactaaa taaacttcct cacaaaaacc ttttaaccca ttatatactc
    11821 tctgcgctct tgaataatgt tgacacaagg caaaaacggt tggtccgctt ccactcatca
    11881 atgcagcatc tgctccgtac ttgatcatac gctctttgat ttgatggatg actggatgcc
    11941 gctttcccgt tatatcttct aaactgtttc ccattttttt gatcatctgc tgataatctt
    12001 gctctcggat ggcttcagca agtccgtgga tgtcttgatg ttggatggct tgcgtatcta
    12061 cttctctaaa cactgtacgt gtagagacac tcattcttgg tttgaccaac acaacccagc
    12121 actgtggcat agaaggtaat acttggattt cttccccttt gccaccgaca aatgccgtac
    12181 ttccgtagac acagtaagga acatctgtac cgacttccaa accgatttct gccagttctt
    12241 ctaacgaaca gcccagattc cataattggt tgatccctct tagagcagct gcacagtctg
    12301 tacttcctcc gcctaatcca gctgctactg gaatattctt cttaacaaag atcttgacac
    12361 cggttttgat attgtattta ttttttagca ataaagcagc ttgataaaca ttgttccgtt
    12421 catctaccgg taaaaatgct ttatttgttt ctacaatgat tttgtcttct gttatttctt
    12481 caaaaaatag atgatctgcc aagtctacgc tcgacatcac catttccaac tcatgatacc
    12541 catcttgccg tctatataat acatccagtc ctaaatttat cttggctggc gctttttcaa
    12601 tcaattccat cctctcaccc attcgaaaag tctaagtcgc aagtattcta tcatagggaa
    12661 aaataaaagt atagcttatg tctgcctagt ggttgatttt gtcatcgttt caacagcttt
    12721 cacagaattt tctcttcata atccaaacaa gttctagtga ttcttataat tctgtgagca
    12781 cttcttctgc caaactcgga acatttcctt tttcttttcc tatcagactg agtagctgca
    12841 tggtagcaaa tccgaaatta cttcctttcc tataagcaaa ataacgtcct tcccaatgat
    12901 cgacatattt taatttcttt tcataagcag actggaagga atagaaaaaa ttaccataat
    12961 tatatacgat atgcatgact ttttcttgaa agaaagaaaa atctgtttct ccaacattag
    13021 ctaatggagc taagcctaga ttggcttccg agtagcccat tttctcgtat acaataaaca
    13081 gattggctaa cagatagtca gctaattcct cagattcttt aggtaagacc cgcaacaaat
    13141 catatcccgc ctgcttcttc gtcatcggct gagcggtgat gaagccaacg actttttgat
    13201 ctttacggac aataccgaca ccgctagata atacatatga tttatcaaat cgtcccacag
    13261 agaagtattt ttctcgtgca ccttgaagcc atttttgtga aacttcattt acagcttgat
    13321 gtaattcttc cggcagttga tcgtaccaag taaaatggta tccttctttc atcaaacgtt
    13381 gatattcaag ttgctgactc agccgagaca agtcttctgt tgctagatca acaatccctg
    13441 cttcacctac tttcgtgaag tgaaaaccca attcatgaag catcactacg tagctttctg
    13501 atatcttata aaacgcaagc tgatagccta aacaatctgc ttgtttcata aactgattcg
    13561 ttgctgcccg catcatttta ggattgccaa ctggatctcc taaaacaaag catttgtttc
    13621 ctttgatctg ataaccaaaa acgacttgat cttggtcgtc ttcttggtaa taaaaataac
    13681 tgtaacctga caagcgaagc tgatggcttg tagctgtgcc tccgtatgtt tccaataaac
    13741 ggtcaaaacg gtctttttcg tactcactgc caagcttcgt agtatcttca gctaaatatt
    13801 gataaagcgc cactaaagct agcaatgaga tgcccaaacc aataagccct gagaaccaca
    13861 catcatctga aggaaacaaa ataaaatgat ttcctgttat tcgaccgact aaaaagcgtc
    13921 cgctgtgata gcctgcaatt gcgtaaacga taaacaaaaa gccgaacaag ctagcatcaa
    13981 agattatagc tccccaagaa taaacgaatt ttgttcgata aaattctttt ctggataacc
    14041 acacagctaa cagaatcaag acatatacca atatcaactg ccaagataac gtacgtgtga
    14101 ttgtatttaa aataccaaaa gatagtaaag caatcgtagg cagaaatgcc tttttgactt
    14161 ttactgaaag tgctctagca aggccgagta ataaaaagcc aaccaataga ttcactgtct
    14221 gatctaaaaa attgaatgag aacggcaata atacttgaaa tagccgactg attgcagaca
    14281 gattagtgat tgttgataga agtaccatca tgatccccgc aaaatagaca gcacttacga
    14341 gcacgatatg cgcaaccttt tggaaaaaca gtcgcggaat gttatctaaa aatcgattga
    14401 ttttgactcc tgtatgtgtg atgaacaata gaagtcccgt cataaaaggt agaacataat
    14461 aaaacaaccg ataatataat agccaaacta ctgctacatc ttgccgaaca ggcaattgac
    14521 tcattcctaa gatcatcaaa acgtcaaaag tccccattcc acctgggacc atcgtcagca
    14581 ttcctatcaa tgtagcaatc atgaacatag gatagatcga aagcatcgaa atatctattc
    14641 tcatcaactt gccgatgatc aaaaataccg caagggctcc cgtccattgt ccaaaggatg
    14701 ccagtagcag accaagcaag ccttttggat aaaactcgaa gaacaatgtc tttctcttta
    14761 gataagcaaa aaagaaaatg actggtgcta aaaaactacc gccgaaaagc cagatccaat
    14821 agcttcgaaa aatactttct gactgaataa aaaagacatc aataaatgta agaaacgacc
    14881 atacagacaa accagtcagc ataaatagtg cgacttttga tacagtggct aaaaccattt
    14941 ttctatttgt ctgctttcca taaaaactag cccgcagact tgctcctacc acaccaccaa
    15001 aaccggcgag attgttgatc gtgtttgtta cccacgcagc aagaagaaac tcttttcttt
    15061 ccatttttgg ttttccttgt ttttctaaga ctctgatcgt cacccaatca tacaacaaca
    15121 tcggagtgac acccagcagt ccagccagaa tcatgagcag gatcgtcatg cggctttgtt
    15181 gtcccatcct ttgccaaata tcttgccaac tcatgccatt agcaatattt gcgacttgat
    15241 ttgccacaaa gactaacacg gagccgaaaa aaatcagttt taaaatcaga ctatgttcct
    15301 ttatccattg cagcatcaag tatcgtttat ttttcacatt ttcccattcc taacttgtat
    15361 acttttgtgt cataccccaa tgttatctta tttattttaa tttttccact ttagacctgt
    15421 tctttgcttg cgatgataga aaaagaaaga tatcctaatg ctaaaggaga ggataacatg
    15481 aaagtaggat ttatcggcac agggaatatg gcacaagcta ttattgatgg tttattaaaa
    15541 aaaggaacag tctcgaaaga agaggtgttc gtttccagtg gacattttga aaatgcacaa
    15601 aaatttgctg ctgaaactgg cgtccatgca tgcaaaagta atcaggaagt tgcatcacaa
    15661 tcagatatcg ttcttttagc cgtgaaacca aaaatcattg aaaaagtatt ggaagaacta
    15721 agagaatata gccagaaagt cttattcatt tctattgctt caggaaaatc attaacagat
    15781 ttagaaacag cgcttgaaga tcctcaggcc tccatcgtac gtgtgatgcc gaatgtcaat
    15841 gtcagcgtag gagcaggcgt ttctgcgatt tgcaaaaatg aagctgccac ggaggaacag
    15901 cttcagcaag ctacgatgat ttttgaagca atcggcagcg tctatccttt agcagaatca
    15961 gatttcagta cattcattgg ccttgctggc agctcacccg cattcgttta catgctgatc
    16021 gatgcgatgg gacggactgg tgtactgaat ggtttgtcca aacagacagc cactgagatt
    16081 gctgcaaaag caattatggg ttcttgtcaa aaattccttt caagtgataa aaatccatgg
    16141 gaacttgtag accaagtttc ttctcctggc ggtacaactg ttgcaggtgt agttgcctta
    16201 gaagaagcag gattcatccc agcaatcatc aaaggaatca atacgacgat cgagaaagat
    16261 cttgagatgt tgaataaaaa ataatgatag caatgttttt tcgtgcctta caaaaaaaga
    16321 agagctgtgg cttttgccgc ggctcttctt ttgtattttt tattatttag cataatcagt
    16381 tgcacgagtt tcccgaataa cgattacttt gatgtgtcca ggataatcca gttcttcttc
    16441 gattcgttta cgaatatcac gtactaaacg aacagcgtct aaatccgaga tttcttctgg
    16501 cttgaccatt acccgaactt cgcgtccagc ttgaactgcg taacttgatt ctacaccggc
    16561 gaagctgttc gaaatatttt ccagacttct taaacgacgg atgtagtttt ctaatgattc
    16621 acttcgcgca ccaggtcttg ctgctgagag cgcatctgct gcagctacaa ggacagagat
    16681 aacagaagta gcttccacat ctccatgatg agaagcgatc gcgttgatta ctactgggtt
    16741 ttctttgtac ttggcagcta attccgcacc gatctcaacg tgagatcctt caatctcatg
    16801 gtcaagtgct ttcccaatat catggagcaa cccggcacgt tttgctaatt ggatatcttc
    16861 gccaagctct gctgctaaga taccagctaa ttttgcaacc tcgaccgaat gcttcaagac
    16921 gttttgtcca taactagttc tgaatcgcag acgtccgatg atcttgatta gatctggatg
    16981 aagtgtatga gcaccgactt caaacgctgc ctcttccccg tattcgcgaa tacgttcatc
    17041 catttcttta cgagatttct cgaccatttc ttcgatacgc gctggatgga ttcgtccatc
    17101 ttgtatcagt ttttccaagg tcatccgtgc gatttctctt cggattggat cgaatccgga
    17161 aagaacaact gcttccggtg tatcatcgat gattaaatcg ataccagtca gcgtctcaag
    17221 cgttcgaata ttacgacctt ctcgtccaat gatacgacct ttcatttcat cattcggaag
    17281 ggtaacgact gaaacagtcg tttctgaaac ttgatctgct gcgcagcgtt gaatcgctaa
    17341 tgataataag tttttcgctt tgcgatctgc ttcttctttg gcacgctgtt cagattcttt
    17401 taccattaga gttaactcat gattcagctc ttcttcagtc gatttcatga tgacatctcg
    17461 tgcgtcctct tttgataaac cagcaatttt ttctagttct ttttgttgat cttcaatcat
    17521 tttttcaacg tctttttctc gctcttcaat taattgctgt tttgaactaa gtgcttcttc
    17581 tttactttca agtgagttct cgcgtttttc taacgagtca tctttacgat tcagcgtttg
    17641 ttcgcgttga atcaaacgat tctcttgaga ttttaattct aacttacttt ctctcagctc
    17701 actttcaact tcagaacggt atttctgatt ttcttcctta gcttctaaca atgcttcttt
    17761 ttttagagtt tctgcttctt tttttgcact atcgatgatt ccagcagctg atgattgtgc
    17821 accagctatt tctttctcgt gacgtgactt tgcgaccata aaccctaaac caagaccgac
    17881 aattaaaccg atgatagcga ggagaatgtt gaataccata aaatccacct ccatactatc
    17941 tttttcattt ttatcatcta tacagcttct atgataaatt attctaaact cgtcaatatg
    18001 catcttcgca tatatgtgta caaaaaacat gcacaacttt attctaatgt ttgtaccccg
    18061 tagtgtcaac tttaaataaa caaagaaacg gctttctact tattcattaa acgatggttt
    18121 ttaaaataaa tgacactagt ttcatatagg taaaccttta aattgagaaa cttttctatt
    18181 aaattattct ttgtagatga acagatattt ttattttatg tacccactcg tataattaaa
    18241 taactcacga tttctttatt caataaaaaa acggcaggtc aaggagagta cctactcttc
    18301 agaccagccg tttttgttta tcataatttt tattcttcta ctaaaggcaa ttcttcttgt
    18361 cctttttcat cttctgggac agcgacttct tcaccaatac cataagctgc tctaactaga
    18421 gcagaaactt ctgccatcat ttcaggatga ttagccatat agattttagc gttttcccgt
    18481 ccttgaccaa tccgatcttc tttataagaa taccaagcac cgcttttatc aacaatgtct
    18541 ttttctactg ccatgtctag aagttctcct tcttgtgaga tccctaaacc atacatgaca
    18601 tctacttcag cgattttgaa aggaggtgct actttgtttt ttacgacttt gatctttgta
    18661 cggttaccaa caatatctgt accttgtttc aattgttctg cacgacgtac ttccaaacgg
    18721 atcgttgcgt agaatttcaa tgcgcgtcct ccaggagtga tttctggatt cccaaacatc
    18781 acgcctactt tttcacggat ttggttgatg aaaatagcga ttgtttttgt tttgttgatc
    18841 gaaccagaga gtttacgcaa tgcttgagac atcaaacgtg cttgtaaccc gacatgtgag
    18901 tcacccattt cgccgtcgat ttctgctcgt ggaactaaag cagcaactga atcgactact
    18961 acgatatcta cagccccact tgatactaaa gcatcagcga tctctagacc ttgttctcct
    19021 gtgtcaggct gtgaaagaag tagttcatcg atattcacac ctaatttttg tgcatattgc
    19081 ggatctaacg catgctcagc atcaatgaaa gcggccgttc cgccattttt ttgtacttct
    19141 gcaatagcgt gtagtgcaac tgttgtttta cctgaacttt caggtccata tacttcgatg
    19201 atacgtccac gggggtaacc gccgacacct aaagcgacat ctaacgcaag agagccactt
    19261 ggaattgtag agatttgttg gtcgattttt tcgcccaatt tcataataga gcctttacca
    19321 tagctctttt caattttttt cagtgcagca tctaaggctg ctttacgatc atctgccaat
    19381 acgataatcc tccttatatt tacaaatcag agcaacattt cattatcctt atcatatctt
    19441 ttttagtgaa aaaaagcaag aaaaaaccga acaaatattc gcatttatca ttttcttaaa
    19501 agctgttttc tcaaaagatc aaatccttgc ataaccgcat tatatctgat agcagcacga
    19561 tctctggcaa agtgatattt ttccgaaacc acgccgtctt tagatgaaat agcgatccat
    19621 actgttcctg cagattgacc ttctaacgtg tccggtccag ccactccggt gaaagctaca
    19681 ctgtaatctg ttcttgctat ctcacgtgct tgaatggcca tctgctccac acattctttg
    19741 ctgactgttc catattctgc taatagctcc ggatcaatct ccagaaaacc agcttttgtt
    19801 tggagcgagt aggtaacgaa tccgcctgga aatacttcag atacaccagc aatgtcgcct
    19861 aatgcggctt ggaaggcgcc ggctgtcaaa ctttccgctg ctgttacagt ctgcttgttt
    19921 tctttcagta gttccactac gacttttgct agcgaattat catcgccgta tccataaaaa
    19981 tattccccta ctcgttcttg gattttttct tctaatgcta aaagagcttg attccctgcc
    20041 tgaacatcat ttgttttcac tgtcagtcgt aatgtcactt catttggttt ggcatatggt
    20101 gcgatcgttg gattgatctg tgtctcaatc agatctttta gctcagtgac cagtcgcgat
    20161 tcaccaatcc cataaaagcg caaaacttta gaaattagtt tttcttcaga aggaaaacgt
    20221 tcctctagta aaggacgtac ttgttccaca aacattggtt tcaactcatt aggcggaccg
    20281 ggaagaagga tatacgcatg tgtatctgtt tgataaaaag tccctaaagc aagccctgta
    20341 cgatttggta gaggaacccc gccttcgata atctgggact gttgcagatt gtttttggtc
    20401 atttttcgat gggttgtttc aaaatatgcc aataattttt tgtatccttc cgtattttgt
    20461 atgagagact taccgaggtg agccgcagtt gcttcttttg tcaggtcatc ttctgtcggt
    20521 cctaatccgc cacataagag tatgagttca cttcttgttt cagctagagc aatcaactct
    20581 tctaaacgct gctgattatc tcctacaact gtttgataat aaacttcgat acctagatca
    20641 gctagctgtt ctgacaagaa agtggcattc gtattcacga cttgtcctag taaaagttct
    20701 gttccgacag ctattatttc tgctttcatt acttcccgct cccttatata gtatacgctg
    20761 tatttttata tttttttatt taaaaagagg gcaaggagct gtgacaaaaa tcttgtcaca
    20821 gctccttgtt ccaaataaac agtgttctcc tgccaaaacc tcggtcataa gtcacaatat
    20881 acataaaaca tgaaaagctg ttttttgaaa ttatcccttg tgactcagag ttagacggca
    20941 ggctcacaac ctctccacta aacgatactt acattgatcc tttgaacaca ccgctgtttt
    21001 tgatgaagta atccacacct gaatagatag taaagatcag acaagcataa agcatgattt
    21061 gatctactgg aataccgatc aggctgaatg ggatattatt gataaacagc aagataatag
    21121 caaccatttg tgtagccgtc ttgacttttc ccggccaagc tgcagccatg acttctccat
    21181 gttcgacaag tagtaaacgc aagcctgtga cggctaattc acgacagaca atgatcgcta
    21241 ctacccaacc aggggccttt ccttggtcaa ccaacatgat aaaagcagtc ataactagca
    21301 ttttatctgc caacgggtcc gcaaacttgc caaaattagt gacaaggcct cgcgcacggg
    21361 caatttttcc atctaaccag tctgtgatac tagctaaagc aaaaataact gcacctacta
    21421 gctgtgtgac agccagtgtg gtgtctccta cgttaaattg tccccaatct aaaggtaaac
    21481 ctacaaccaa aatgaaaatc gggatcatac aaattctaag tacagttaat ttatttggta
    21541 aattcacact atcgactcct ttcttcccta ttctacgtga ctatcagcga agtttccacc
    21601 gatattattt acttttccaa atgaatttat tcaaaggagt atttttattg aaattgatct
    21661 ttttgaatta acgaagaagg accgcgacac agatttgtca cgatccttct tttttatacc
    21721 tacaagtaaa cagatgcgta aactaattta tacgatcatt ctgcgtattg aagagtaagg
    21781 ttcacatttt ttggactctg attgttttcc cctggattaa ctggtacgga ttctccattc
    21841 gctttgatcg ttgcattgct tgctgcacca atcgtgatgg tcgcatttgt tgctgtttcc
    21901 ggtaacacgg tactctgtgt ttcatttgct gccagtgtcc cttgataaac ataggcattg
    21961 tctactagca cacctaacca tacacgattg acagcagtaa attcaagggt tacaggcttg
    22021 gtagcgtctg tgatatttac tgcgatggca ctgccagaat cttctcctgt agctattgcc
    22081 attttctttt cttcacttga ttttgttgtt gattctactg tagaagactc tttggtctgc
    22141 gtgctttgct ctgaggaaga ggactcctcg actgatcctt ctactttaag cgaagaagct
    22201 gtgtctccga ttattggatc agcttgacga tcttgccaag tcatatagcc cacgacaacg
    22261 acaatcgcca aagcaaccaa tcctaataaa atgacaggaa gacttgtcca aaagcgactt
    22321 ggatgcgatt cttcctgatg cattgctttt ctagaagtac ttactgtttc aggctgcgga
    22381 cgttcaggga ccgatcctgt caatttgctt ttcccatcaa aaacatcgac taaatggtct
    22441 ccatcttctc ctacagcttc agcgtattgc cgaataaatg cccgcacgta aaatttacca
    22501 ggcaattggt caaaatcatc attgtcgatt gcttccaaat aacgtttttg aatttttgta
    22561 atctgctgta attcatctaa agatattttt ttattcaggc gtgcttgccg taatttttct
    22621 ccgatggttt caaaagccac tcgttcatct tctccagtct gtttgattct ccgaagcatc
    22681 tgctgttgca acgaaagacg ttatgtttgg gtgctgtgac aaaagtcatg tcacagctcc
    22741 ttttttccga ataaacattg ttctcctgcc aactcctcgg tcacaagtca caatatccaa
    22801 taaatataag aagctgtttt ttgaacttgt tccttgtgac tcggagttaa acggcaggct
    22861 cacgacctct ttcttaaacg gtgttcaatc agctgttcct cggtattagc tcaaaaaccg
    22921 gcaaaatata aggagatatt tttccgtatg tttctctaat actcaggtca aaacgctgat
    22981 ttcacaacct cttccttaaa cggtgttcaa aagcttgcat ctgcggtaat aagtccaacc
    23041 aagttaaaaa cattaagaag ctgtttttac ttggctgttc ttattacttg atgcagggca
    23101 gctttttcac aacctcttgg tacacggtgt tccagaagta gcttcttcgg caataagccg
    23161 aaatatcaca aaaatttgag aagcaatttt cgtaaattcc ttcttatttc ttgaagctga
    23221 ccacttctgt cacaacctct ttgattactt cgtttgattt tattttaata taagttgatt
    23281 aaaagagagc atgatttgtt ttattttctc actaattttt ccaacacaac taagaatacc
    23341 ataaaagctg attttggagg aggttcctac tacaagtcta aaacgaaatt taagactttt
    23401 cctcataaaa tactggttta ttttggtctc atgtaaaact cgctgaaggc ctcttcactt
    23461 ataaatgctt ccccagctgc cagtacgtct ttcatttgga tcgaatcgat gatttcagga
    23521 agatcaaata aagttcgatc gccaaataaa ctttgcgtaa attgattggc aatatattcg
    23581 atcgaattta atgattggaa atattgtcca agcattttct tcttcagcaa atcgagattt
    23641 gtttctgaca cttccggatc atcagcgaat ccaagaatga tttctttgac tttttctgct
    23701 gctttctctg gttcgtccgt atctccgctg aagtccgcaa agtgaaattc acgatccaag
    23761 ctgaattcaa aaccaaagct gtcatcgata attccttgat tgtacatcgc tagatagttt
    23821 cgagaagtat tgcctaaaat catttggaac aacagattga ttgctgtttt atagcgtaac
    23881 agttctgttc cttcttgcgg caatgtgtct aatcctttaa taccaagtac aaatttatct
    23941 cgtgtaatgg ctgcttccaa tgcactttgt ttgatgattt cttttgtgtt ttcagggaaa
    24001 taacgtacaa tttcttgctt tggagggaaa ttttttgctt cttggttttc ccgaataagt
    24061 ttcatcaatt tctcaggctc catcttacca actacaaata aaaccatatt gcttggctga
    24121 taaaacgtcc gatagcacgt atacaagtct tgagctgtga ttttgtcgat actttcaacc
    24181 gtgcccgcaa tatcgatatg caatgggtgt gtaggataca agttatttag tatgccaaaa
    24241 aacatccgcc agttagggtc gtcttcatac atttggattt cctgaccgat gattcctttt
    24301 tccttgttta cagtttcttc tgtgaagtaa ggtgcttgta caaaatcgat cagagtcgtc
    24361 agattctttt ctacctgatc cgtcgtagaa aacagatagc tggttttcgt aaaacttgtg
    24421 aaagcattgg cggaagctcc ttgtttccca aataattgaa agacatcacc atcttctttt
    24481 tcaaacaact tatgttctaa aaaatgggca attccatctg gaactttcac cttttctttt
    24541 tcaccatagg gaataaattc gttatcgatc gaaccataat tcgtactgaa caaaccatat
    24601 gtcttgtgat agtcattttt aggcaacaaa tacacggtca aaccgtttgg cagtacttca
    24661 tgataaagtg tttcattgat ttgctcatat tctgttttat tcatttgcat ttcctcctgc
    24721 taaaaagaag attgcctgca attcgatttg ctcagctact tgttgtatct ctttgatcgt
    24781 tacgctctcc atacgtttca accattcttc atcagataat tttgtttccg gcagccagct
    24841 gtccaaaaaa gaagcttcga ttgccgcttg cggagagtct agagaaagca gatattggtt
    24901 tctcagcatt gcttttgtct gagacagttc caactctgtt atttcaccat ttcgcaaact
    24961 ttctaattgc tcgtgaatca gatgaagtac ttgatttcgg ttcttttcat cgataccggt
    25021 ttggacactc atgaatcctc ggaacgtatc cacgctacta gaggcataat aagctagact
    25081 ttctttctct ctgacattca tgaaaagttt agagtgagga aaaccgccaa ataaaccgtt
    25141 aaagaccatt agcgcaaaac gttctggttc atcgtaatac acatttgtct gataagctag
    25201 gttcagcttt gcttgaacga ttggctcacg tacttgtcgt tccttcacga tattcacttg
    25261 aggttgggta taaaacagat ctggataaac tgtttcccgc gactcaaatg gtaattgccc
    25321 aactgcttca gctgctttgt ccggatcaac atcgccgaca acaaaaatgt cgatttgatc
    25381 atttgccatc atttcttgat agtaggacgc tagcgaacta gcagtgatct tgtccaaatc
    25441 ttcgactacg cccaaccctg ggatcttttg ttccgggctg ttttcaaaat aaagttcttg
    25501 caaggcatag gaagcaaaag tttgtttatc ttccttcaaa ctttccaaat atagacgcag
    25561 attgttcttt tccaagtcaa acgtcaactg atcaaactga tgatttttaa tatttggata
    25621 aaataaaacc tcttttaaaa aatctaccgc ttgagaaaaa aggtttggat cattgacata
    25681 ttcaccattc acaaaactga tccctgcgtt tagccaatgt aaatttcctt tttttctaac
    25741 tgataatcca aagcttgcgc cataaagctc tgcaagttgc gcacttaact ttgtctgatc
    25801 cggataattc aaactatttg tttccaacat gctgctgaga agtgaacgtt tcgtgatgac
    25861 ttcttgttgc agacgcgcag taaaacggat aaaaatccgg atcgttttgt attgttttgt
    25921 tggtaagaca tgaagattca cgccttctgc caattgatac accatattta tatgctcctt
    25981 ttcacagttg attgcccgct tactctttta atgggtcaac ctgccttttt tacttattcc
    26041 tctgcttgaa atttataagt tcaggcaatt cgccgttttt tctaaatgat gttacacttt
    26101 atagtatagc atagatgttt tttatcaaaa gaaacagacg gctaaaagcg tatattgttt
    26161 ttttattttt tatttttcga tatactaaag acaaataaaa gaagggaggg tcattcatga
    26221 tcaaagaatt caaagagttc atcatgcgtg gagatgtctt ggatcttgct gtcggcgttg
    26281 ttattggtag tgcgtttaca agcatcgtaa accaagtggt gcaaggcttg atcacaccac
    26341 taataggttg gtttgtcgca atgatcacag gtacttctga tttagaagga gcactttcaa
    26401 ttcttgactg gtcgccgaca aaaggcgtca catttgcgtt tggtaacgtt attagtgcta
    26461 tcattacatt tctgatcact ggttttgtct tattcttagt tgtcaaagcg gcaaaccgtg
    26521 cgaagaattt acgctccaaa gaagagcaag tggaagtgga agaaactcct actgcagaag
    26581 attatcttag tgacatacgc gatcttcttg ccaaacaagt ggcagaagac aatacacatc
    26641 cagtagagca aacaaatctt tccgatactt ctaaataata aagaatctaa aaaaaagaaa
    26701 gccgattttc agtgtcggct ttcttttttt agattcttaa cttgctactt gttccttttt
    26761 catgtcgata tgtggaatat catcttccaa atacacatct gagattgcct tgaatccaaa
    26821 cgactcataa aaagcttgta ggtattcttg tgcgccgatc acgattggtt tttctggaaa
    26881 atttttctgg atcatgttaa tcgtttcttg aacaatcttt cttcctaaac catctttacg
    26941 gttgtcctga tgaacaagga cacgtccaaa atggatactc tccgcttctt catatactcg
    27001 cgtgtaggca aggatcttcc catcagtatc ttgaaaaaac gtatgccaag cttgtccatc
    27061 aatgtcatca acttcttgat aaggacactg ttgttccaca acaaaaactg cgattcgttc
    27121 tttgacgatc gcgtaaaact ctgctgttga caattcttca aatttcttta cctgatattc
    27181 cactaagcaa acgccccctg cttttttatt cttagcatac cgattcgcac acaaaaaagc
    27241 aaacgaacct tacgaatctc aggctcgttt gctttttaaa ctgcttcagt taaaaattcg
    27301 atctcaactg tccgagtaag tacatctgaa tagctgtaag atactcgttc aaaagaattt
    27361 tcttcaggat ccaaatcaac aacgaatact gaaggatagg tttctgttaa aacgccgcgg
    27421 cgttctgttt gacgctttct tcctgtctga gcaaccaaca tgattgggct gccgatacga
    27481 ccttctaaat ctttcttgat tgaagctaaa gttgttggca tacagttcac ctctactatc
    27541 aactatacca catattttta aaattttcaa gtttaccata aaaaaaaaag aaatgcaata
    27601 aaaataaata agaagctcat taagattttt ctaaattatt cctcattata aaatttcaag
    27661 tgttttttta ttttgagttt tactctcccg taagcgctca ttacttgctt gtgatccatt
    27721 gctaaacatt cagccatgtc tacaaaatca acgccttgca tataataatg aaaaatttta
    27781 gcttctattt ttgaaaactg gatttcgcaa ttttcgattg cttcttttaa taaaagatgc
    27841 tgaatcgcta aaggttcttg caatgtgatc tctgctaaaa agtctggtcc ttctgtctct
    27901 attttttggt cgattgagac ttcatctttt tgatcacgac gcttcagcgc gttctgcttc
    27961 ctcaacagag aatagatttg attgcttata atgcttttat aataacttcc aaagctaaca
    28021 cctttgcttt tttcgtaagt tctcaatgcc tgatataaag cgatccttgc ttcttgttcc
    28081 caatcctcag aaacataatt tcttaaataa taggtgtttt tcaaacgtag taaaagtgga
    28141 aaatattttt gatacagggc agcaaatgct tcttcgtcac cattacgtgc agcgcgcaat
    28201 aactcctcag acataaaata attcctccat aaattcgttt atggaggaat cttatcatca
    28261 atgattcatc gaactgatta gaacgatatg ttatttattt tttctcatta aatcgtccaa
    28321 tttttgggac aattttttta actgctcttc attccatgga gaattacgtc ggaaatcaat
    28381 aaaatgcata tctttatttt cttgggctat tgttttttcc gtttttttta tcgtcttata
    28441 taattcataa gcagaagttc gtaatgctcc ttgggaaaag atcatccact gttctgctaa
    28501 atcgcttgtc gccactgtca cttgtgtcaa acggtcattc agctgaccag cgattctctc
    28561 gatataactg tcagccgttt cgtcttcttt cgtaaaaaca acatccaact ggtatttctt
    28621 ataattttgt tggatgcccg gaactagttg ggcatcgaag acaacgatga cttccattcc
    28681 ttcatatttc gcataatttg ataaacgatg taaaagagct tctcttgcat ccgccatttt
    28741 gtcctgcttt ttaagctgga caagttccgg ccacgaacca atcatgttgt agccgtctac
    28801 aatcaacagc tgttttttca tggtctactc cccctgacgt tgggaaaaag cctgatacat
    28861 aagcaaacca gcagcaacac tggcatttaa gctctggacg tgcccggtca tcgggatcgt
    28921 cagcaattca tccatttctt ttttcaatcc tggactcatt cctttgcctt cattaccaat
    28981 gactaaagca attgatcctt tggcattcca tttttggaag tttgtgccgg tcatatctgt
    29041 gccaaatacc caaaatcctg tttctttgag ctgttgcaca gcttgtacca aattcgtcac
    29101 gcgtgcaaca gggacatgtt cgactgcccc agtggaagtc tttgttacga taggtgtgat
    29161 tccaactgct cggtgttttg gtatgatcac accatcgacg ccacttgcgt ctgctgtccg
    29221 taaaatcgac ccaaaattgt gtggatcttc gatgctatcc aaaatcaaaa agaaggggtc
    29281 ttcttttttc tcttttgttc gttcaatcag ttggtcaagg gataaatatt cataaggtgt
    29341 aatcgcaaga acgactcctt gatgcacgcc gtgatcgctc aaagtttcta atttttgttt
    29401 aggaacccat ttgactggca cagcttgttc acgtgcgatt tctttcagtt cttctacttt
    29461 ttctcctcgt gaatcttctt gaagaaagag tttattccct cgcccttgtt gtagcgcttc
    29521 tactgcggca tggtgcccaa agacgaaatt atcttctaat gcttcttcat tttgatcaga
    29581 aactgactga tttcgtccag tttgcttttt atttgaatat ctattttgtt ttttgtcttc
    29641 aaatccacgt ttcgattgat gtttgtttgt tctttcttta cccatttttt tcaccaactt
    29701 tctgaataca ccagcggatc aattcttcca tccgctcggt ttgttttgtc aaatgcagat
    29761 agcccatgag cgcttcgaac cctgttgcga cccgatacgt cgtaacatca gcattttttg
    29821 cgctcgtatg gcttttagca tttcttcctc tgcgataata aagttcttct tcttctgtca
    29881 aaagatgttc ttccagcatt tgctgaatca aaaaggcttg tgcattcgca gaaacataat
    29941 tcgttgccgc acgatgcagc ttattgggtt ttgtttctcc ttgatcaatc aggtggtcac
    30001 gaacatagat ttcgtaaatc gcatctccaa cataagccaa ggctaaaccg ttcaattgtg
    30061 tgtaatctct catgcttctc ttctccatcg ggttccttgc ggtgtatctt ctaataagat
    30121 ccctttttct ttcaacaaat cacgaatttc gtcgcttcgg acaaaatcac gattgcttct
    30181 tgcttgattt cgttcttcga tcaattgttc aatctcttca tctaataact cttctgctgt
    30241 aaataaaata ccgaagatcg aaagccattg cgagaataaa gcttctcctt gtgccaagac
    30301 agcggcagaa actttcggct ggtctagata agtgttcagc cacttagcaa gctcatagac
    30361 cactgtgatc ccatttgctg cattaaaatc atcgtccatt tcttcgatga atcgctgttc
    30421 taaattttct aacaaagcga ttttatccgc atcatctgcc aaattctctg aggcagtttc
    30481 ttggcggaat ttcaaattgt caaacgtatt cttcagtcgt tgtaaattgg tggctgcttc
    30541 tttcatcgtc gtttcactgt agcggatcgg acgacggtat tgtgtggtcg ccatgaagaa
    30601 acgcaagact tgtggatcaa cgtttttgat catttcatgt acagtgatga aattccctag
    30661 agatttactc attttttcat cgtcttcgcc aatcgttaca tagccattgt gcatccagta
    30721 attagcgaat tttttccctg ttttcgcttc actttgtgcg atttcatttt catgatgagg
    30781 aaattctaaa tcttgcccgc caccatggat atcgatcgtt tctcctaaat gttttgttgc
    30841 cataacggaa cattcaatat gccaaccggg acgtcctttg ccccatggtg aatcccaaga
    30901 aatctcgcca ggttttgctt gtttccataa agcgaaatct aatgggtctt ctttttgctg
    30961 ctgttcttcc cctgtacgct gacttgctcc gacttccagt tcatcgatgg attggtcact
    31021 taatttccca taattttcaa atttacgggt acggtaatac acatcacctg aagattcata
    31081 agcatagcct ttttcaacca acacttgaat aaatgctaaa atatctggca tatgatccat
    31141 gactcttgga tgcagtgtag caggctcgac attcaacgct ttcgtatctt cttcaaatgc
    31201 tcggatgaaa cgatcagcaa cttctggtgc tgtaatacct aattcgtttg ctgtacggat
    31261 gattttatcg tctacatccg tgaaattcga tacgaaatcg actttatagc ctcgatattc
    31321 aaaatagcgt cgaattgtat cgaaagcaat ggtgctacga gcatttccga tatggatata
    31381 attatacaca gtcggtccgc agacatacat accgacatgt cccttttgta tagggatgaa
    31441 ttcttctttt tttctggtca atgtattgta gatttttatc attctgatct cctcactttt
    31501 tctcctttga ttcgaacgac ttttgctggt actcctaccg ctgtagcgtc gtctggaaca
    31561 tctttcaaca caacggcacc tgcaccgatt ttggcgtttt ttccgatcgt taccggtcct
    31621 aatatctgcg cattcgcaga gagcatggct ccttgtttaa cagttgggtg tcttttaccc
    31681 gtttcttttc ctgtgccgcc taatgttacg ccatggaata aaatcacatc gtcctcgatt
    31741 tctgcggttt cgccaatgac gattcccatt ccatggtcga tgaacacgcc agttccaatc
    31801 gttgctcctg gatggatctc aacaccagtt aggaaacgcc agaattgagc attgatcttc
    31861 gccaataaaa agagccggtg ctgatataaa aaatgagaga aacgatgcca aaacaaggca
    31921 tgaaaaccag ggtatgtcaa aacgatctca gctgtcgaac gtgcagccgg atcgtttctc
    31981 tttgctgcat caatcgctcg ggtaaaccaa ctcatgactt tccactcctt tcgtaagaat
    32041 tttatgtagg aaatcatctt cttataaaga aaagaagcgc cttttaaata agtttcctta
    32101 tcaaaagacg ctttccgcgt ggttccactt ttcttcataa tcaatgatta tctctgacag
    32161 ctaacgcctg ttatacgttc ttggctactt tgtttcctat aaaacagttt ttcctgtttt
    32221 ctgtgtttcg ccaagaccac tcccagaggc atttcggcaa ccattcattg ataccttgca
    32281 ccacccggta tctctcttgt aatgatcagc tgcttacttt tttctgttca tcgtgtttag
    32341 ttcagtactt gatttaaatg tgctaatgat ttttcacgac caagcaattc gatcgtttca
    32401 ggcaattctg gaccatgcat ttgtcctgat acagctacac ggattggcat gaaaagattt
    32461 tttcctttta cgccagtttc tttttgaaca gctttgatgg cagctttgac agttggagca
    32521 tctactactt ccatttgttc tagctgtgct ttgaatgcgt ggagaacagt aggaactgtt
    32581 tcaccagcaa gtacttcttt tgctgcttca ttcaatacag gatgttcatt gaaaaatagc
    32641 gtagacagat caacgatttc tgcagcatag ctcatttgag gttgatacag gctaacgatc
    32701 ttcttcaccc attcgattgt cttttcatct gggttttctg ctacacgtcc atcagcgatt
    32761 agataaggca gacacatgtc agtaaatgca tctagatcca ttgctttgat gtaatggttg
    32821 tttacccatt gcagtttttt cgcatcgaaa gctgcgggtg atttacttag gcgttttgga
    32881 tcgaacattt tgatcaattc ttggtgttcg aagatttcgt cttcgcccac aggtgaccag
    32941 cccaataatg caataaagtt gaacattgca tctggtagat agcctaattc gcgatattgt
    33001 tcgataaatt gcaagattga ttcgtcacgt ttgcttagtt ttttacctgt ttcactgttg
    33061 atgatcaatg tcatatgtcc gaaggttggt ggtgtccagc caaatgcttc atagatcatc
    33121 agttgttttg gtgtattggc aatatggtca tctccacgta aaacgtgagt gattttcatc
    33181 aaatgatcat cgaccgcaac agcaaaatta taagttggca taccatcgcg tttttgtatg
    33241 acaaagtcgc caccgatatt atcggattcg aacgaaatag cgcctttcac catatctgta
    33301 aatgcgtatt ctgtattgcg aggcacacgg aaacgtacaa cagattctag accttgtgct
    33361 tctttttctg cttgttcttc aggtgtcaag ttcgcacatg ttcctgcgta atgaggcatt
    33421 tctcctcttg cgcgttgtgc ttcccgttct gcttctaatt cttcttctgt gcaatagcat
    33481 ttgtatgctc gattgctagc aagcagctga tcaatcagcg gttgatagat ttctttacgt
    33541 tcagattgac ggtaagggcc gtattctcca ggattttcag gagattcatc ccaattcatt
    33601 cccaaccaag caaggttttc caactggctt ttttcgccat cttcgatatt acgtttttgg
    33661 tcagtatcct caatacgaat aataaagtct ccatcattgt ggcgggcaaa caaataattg
    33721 aataatgcag ttcttgcatt tccaatatgc aggtgcccag ttggacttgg tgcatagcgt
    33781 acgcgtactt tcgtcatttt cttcctcttc tttcaaacta gactttgact aacagcaaaa
    33841 attccgtcag ttacggcttc tcaaatatca tttatttaaa gccactcagc acaaatttta
    33901 ccttttctaa ggtaaatagt aaagccaact ggttgaattt ccttaccagt tggctgaatt
    33961 tcttttttat tttgattgtt tagtcttttt ctctgtctct tttttattgt cgattcctcg
    34021 tcctgcatgt gcaggtttag caaagatcat gcgtccagca gctgtttgaa gggcacttgt
    34081 tacaacgact tcgatacgtt cattcatgta atgctgtcca tcttctacga caaccatcgt
    34141 gccgtcatca aggtaggcta ctccttgttg gcgttctgtc ccggctttta ctactagaac
    34201 attcatcgtt tcaccaggga tgacgactgg ttttacggca tttgccaacg cattgatatt
    34261 aagaacaggt acattttgga attcagagac tttattcaag ttatagtcat tggttacaat
    34321 gacaccatct aaaagcttag ccaatttgat caatttgctg tctacttcac tgatatcttc
    34381 aaagtctcca tcgtacattt cgacagatat accctcttct ttttgcaagg catttaggat
    34441 atctaatccg cgacgaccac gcacacgttt caaactatcc cctgagtccg cgatgtattg
    34501 tagttcgtaa agaacaaaat tagggatcat caaggttcct tctaagaatc cagtctttgt
    34561 aatatcataa atccgaccgt caatgatcac gcttgtatcc aagattttat atttatggaa
    34621 attttctcct gattttcgtt ctaacagttg actctcaact tgactagctg tttcttgctc
    34681 gatttttttg cttcgtgaac caaaaacttt cttccattct tcgattcgtg tcgttcccat
    34741 tctcaatccc agatagccta agatgatcat cagtaaaatc ggcaatacac tattgacgaa
    34801 tggaatttcc atattgtaaa gaggggtcga gatcacaaca cctaaagtca atccgatgat
    34861 cgcaccgatc gcaccgaaga gaagataagt caagctgacc tcacttagtt tttgttctac
    34921 ttgtttgata atagctgaga tatgtttggc caacagcaaa gataataaaa agaaaataag
    34981 tgcgcctaac aggctgttgg taaatgtatt attcagccat ccgtttgact gttgaccgat
    35041 tgcctgccaa gctgagggta aaaatgaaat acccaagctt gcgccgacaa tgatcatcag
    35101 caaagtaatg acacgttttt gcatcgtttt tcctccgttt cgacaatcat ttattagtta
    35161 gcggaacaca cgcttcaacg tttcagccaa agttgctact ccaactatct cgattccttt
    35221 tggcgcttcc cagttaccta agttgttttt aggtaggtat acttttgtga agccgagttt
    35281 ttggacttct ttgactcgtt gttcaatatg actgacacgt ctgatttctc cagtcagtcc
    35341 aatttcaccg atgaaacatt ccgttggctg tgttcctttt tctttatagc ttgaagcaat
    35401 actgacagcg atagctaaat caatcgctgg ttcatttaat tttaccccac ctgcagcttt
    35461 taagtaagca tcttgattct gaagaagcaa cccagctctt ttttccagca ctgccatgat
    35521 cagagatacc cgattgaaat caagacctgt tgttgttcgt ttggcatttc cgaacatagt
    35581 aggtgtgact aatgcttgga tctctaccaa aattggacgt gtcccttcca ttgccacaac
    35641 gatagcagag ccagttgcac cgtctaggcg ttcttctaag aaaatctgtg aaggattagc
    35701 gacttcttcc aatccatgct catgcatttc aaaaatccca atctcattcg ttgagccaaa
    35761 tcggtttttt actgctcgca aaatccgaaa actatgatgt tgttcacctt caaaatataa
    35821 caccgtatcc accatatgtt ctaacattct tgggccggcg atcgagcctt ctttggtaac
    35881 atgaccgacg ataaaaatgg ctatcccatt tgttttagcg attttgagta gttctgcagt
    35941 ggtttctctt acttggctga cacttcctgc cacacttgtg atgtctggct gcgtcatcgt
    36001 ttggatggaa tcaatgataa cataatcagg ggagatatgt tcgatggcac gactgatttc
    36061 gttcatatct gtttcagcgt atagataaaa ttctgtgttg atacttccga ggcgttccgc
    36121 gcgtaatttt atctgctctg cgctttcttc gccggataca tataagactt ttccgcctat
    36181 ggctgctaat tgctgagaaa cttgtaacag caaagtggat ttcccgatac ctggatcgcc
    36241 accaatcaag accatagacc ctggcaccac acctccgcct aacacacggt tcagttcttc
    36301 taattttgtt ttgatccgag gttctttttt agggacgacg tctgcgattt ttgttggttt
    36361 tgctttttct cctgtcaaac ttgtccgagt tctgcggtcc gttgtatctt gttcgatttc
    36421 ttcgaccatc gtattccatt ttccgcaatt tgggcagcgt cccaagaact taggcgacac
    36481 atacccgcaa ttttggcaaa caaattgtac ttttgatttt tttgccattg taccgcctcc
    36541 ttttatccta atcatgagac attaaatcaa gcttaagtat ctttaacgtt ttatttactg
    36601 gaagaaccaa atccgccttg acgttgagca gtcgtttgat cttgatctgc ttttaaaaac
    36661 ggcaaaaaga tcccttggcc gatacgctcg ccttttttga tcactacatc cttgaaacca
    36721 aagttggtga attggaacat gatatggcct tcatttgcct cattattgta ataatcactg
    36781 tcaatcacac ctactccgtt tgtcaacact aaaaaccgct tcaacggatt gcttgaacgg
    36841 ttagcaagct gcaaaaattc atcttctccc atgtaagctt tgattccagt aggcacgagg
    36901 gttggtttca attgtttttc attttctgat acatcttttt tcaatccatc tgcgactgtt
    36961 tccaacccct ttttccagcc tgctttccag atacttggaa tcacgacctc ttctgctgct
    37021 tcaaaatcat agccagcagc atgatgtgta gcgcgctgcg gtagattgat attttgatct
    37081 ttatatttag aaataacttc aaatcctctt tttctcaccg gttttcctcc ctaaataaac
    37141 tactcctatt ctaacatatc cgaggctgta aaagactttc tttacaaatt cttcaagaaa
    37201 ttctgctatc ttaaagtaaa ccattaattc gttctttttc caaacgactg caagttgtac
    37261 aaaaaaacta ttttttacat gactttactc gtcaaaacaa gtagaataga acgaaattaa
    37321 tgagtggata tttgattgag gtgtcacatg cgtaaagaaa ttaaaaatgc aaaacgaatt
    37381 attgtcaaag taggaacaag tacattgatt tatcccaatg gaaatatcaa tcttgcggca
    37441 atcgatcagt tagcttttgt cctttctgat cttagaaatc ggggaaagga agtgatctta
    37501 gtctcatcag gagctgtagg cgttggtatg cataaattac atttgtcaga acgtccgact
    37561 ttgattcctg aacagcaagc tgcggcggct gttggtcaag ccgaactgat gaatatctat
    37621 aatcaaagat tcagtgtata taatcaacag acggcacaga tcctcgtgac aagagacgtg
    37681 gtagattatc ctgaaagtcg taaaaatgtg aggaatacat tggaacagct tttgattatg
    37741 gacacgatcc ctatcgtgaa cgaaaacgat actgtagcag tggatgaatt agatcatctg
    37801 acaaaatttg gcgataatga tcagctgtca gccatcatgg caaaacttgt cgcggcagac
    37861 cttttgatcg tcctttctga tatcgacggg ttttattcag ataatccgac tacaaatccc
    37921 gaggctcaga tgtatcgcta tatcgataaa atcgatgaaa agattctggc acaagcaggt
    37981 ggtgctggta gcacatttgg aacaggcggt atgcagagta aattaaaagc cgcttctagg
    38041 atatttgaag caaacagcgc aatgatttta gcaaatggaa aaaatccggc aattattttt
    38101 gatattcttg caggaaaaga agtaggtact ttatttaagg aggacttgaa atgactgact
    38161 tgatgcaatt aggacaacag gcaaaagata gcgcgcaaca actagcactt atgcctacaa
    38221 accgcaaaaa tgagctactt gttcaaatgg cacaaaccat caaaaagaat caacaagcga
    38281 tcattgaagc aaatcataaa gacttagaaa aagcagtcga aaacaatatc agtgaaacga
    38341 tgcaagaccg cttgcagctg acacctgagc gaatcaatgc catggctaca gagatcgaaa
    38401 aaattgcttc gttagacgat cctattggaa aagtagatga gatgtggacg aacaccgacg
    38461 gattgagaat cggcaaaaag cgcgtgccac taggcgtgac cggcatcatt tacgaatctc
    38521 gtccaaatgt cacgacagat gcagctagtc tagcattcaa atcagggaat gccgttatcc
    38581 ttcgcggtgg caaagaagca ttcttctcga atcaattatt agtccaatta ttgcagcaag
    38641 tactggtcag tgctggcgaa tctccttatg ccatccagtt tgtcgatgat acttctcatg
    38701 aaactgcagg acagttgatg cgattgacag aatacttaga tgtgctgatt cctcgaggcg
    38761 gagcaaaatt aatccaacga gtcaaagaac aggcaacggt acctatcatt gaaacaggta
    38821 caggaaataa tcacgtatat atcgataaag aagcacagtt atctatggca gtgaacatca
    38881 tcgtcaatgc aaaagcttcc cgtccatctg tctgtaacgc ggcagaaaca ttgctgatcc
    38941 attctgagat cgcacctttt ttcttgccag ctattgaaaa agaactggta gaacacgggg
    39001 tctctttacg tgcggatgct cgtgcgttag aatatctaga gacggctgct ttagcagaag
    39061 acacagattg ggatactgaa tatctagatt atatccttgc tgtcaaagtg gtcgattctt
    39121 tggatgaagc aattgcacat atcaatcggc ataatactaa acattcagaa acgatcgtga
    39181 cagatagtta tgcggctagt caacgtttct taaatgaagt ggacgcggcg gctgtctatg
    39241 tcaatgcctc cactcgtttt acagacggat cggtatttgg ttttggtgca gagattggta
    39301 tctctactca aaagttgcat gcccgtggtc caatggggtt aaatgaatta acttccacaa
    39361 aatatatcat ttatggcgat ggacaaatcc gcgaataatg tttcgtgtga aacattattc
    39421 ccgccttttg ccttttttct gtaaaggttc tatccaacta gaaaggtgtc aatatgtttg
    39481 aaattttaat gatttttttt atttacctta tttctctgaa tatcgctgct tttctgggag
    39541 tcggtatttt atcccttttc tttcaattca aaaaacgaag catagggagt caacgggaaa
    39601 aatggtcgca gtattttgac aaaatcgggc aaaaagggct ggttacacgc ttgcacatta
    39661 gttacatggt tgcattatgc ttattagcga caatcaatta ctattctttt ttcgatcatt
    39721 caatcgctta tacgattacg ctactgattg ctgggatttt tcacttatct tataaatatc
    39781 agctgaataa aaaccacttg aatcggactt tccgctaaaa aatatccgta cgataagtag
    39841 agtgccttga agatcggcaa aaaactgccg gactttttag aggctgtgac aaagattttg
    39901 tcacagcctc tttttccata taaacggcgt tcgcctacca gctgcctggt aaatctgttt
    39961 cctcttcgtt aagaagcgaa aatccgtcaa atgtttcgcc atcataataa cgaagcagct
    40021 catccatgga caagttcatc gttttttctt tttcttcttc cgctgattca gccagtagct
    40081 gttcccgata cacctgatac ataccagcta ttttttgctg catcgctttt ctagtttcac
    40141 cttccgcatt gattattggc aattctttga tatagccacg ataaatcaac cgatcatctg
    40201 aaaattcaat gggtaattca acgagtgtca acccgacttc ctgaaaatat tcttccagca
    40261 tctattatcc ccccattctt tttcttaaat gaattttagc ttacctactg aaaattttcc
    40321 agtttcatta ttttgagaaa aaaagggaat gtgacaaaaa tcgtgtcaca tccccttttt
    40381 ccgaataaat ggtgttcttc tgccaactcc tcaatcataa gtcacaatat tcaacaaaca
    40441 tgagaaactg ttttttgaaa ttgttccttg tgattcagag ttaaacgaca gtctcacaac
    40501 ctcttggtaa acggcgttca aaagcttgca tctgcggtaa taagcccaac caaacgaaaa
    40561 atatgagaag ctatttttgt ttggctgttc ttatgacttg atgcaggaca gctttttcac
    40621 aacctctgat ttcttattct tcttcatttg tagcaaaata attacggtga taaatgatac
    40681 aacatacact gataagaaca atggcacctg caaaataaaa cgcatagctt tcaccaatat
    40741 gttcaccaat cacacctgca ataaatggtg ctaaagctcc accgaaaaag cggacaaaac
    40801 tatagctgga cgaagcaacg tttcgttgca gacctggtat ttccatagca atcgtcgtca
    40861 acagtgtatt gataattcct tggaaaaatc ctgctaacac aatgctgatc ggtacaagga
    40921 ctggcataga agttccagct cctaataaca gcaaacaaat aatgaataag acaaatgctg
    40981 ctaaaattgt ccgataagtc gtaaacttcc gctcaagagc cggtgcaacg aaaatcgaag
    41041 caatagccag aagcacaccc cagccaaaaa atacaaaacc gacttctaat tcagaatagc
    41101 caatcaataa aaatggcgca tacgcaagta aagtaaagaa tccgaaatta tataacaatg
    41161 cgattagtcc aactgaacga agtttatgat tgcttaacgc tttgacacct tcccaaaggc
    41221 ctacttttct ctttggtttc tcagtttcag gtaaaagtac cgctagggct ataaaaccgc
    41281 aaaacatcaa aaatgctacc ccaaaaaatg gataacgcca tgaaccactg cccaagaccc
    41341 caccgactag agggccaaca gccattccgc agccaagtgc tgcttcatac atcatgattg
    41401 ctttttctgt gttaccaact aaaatactga cgatcgttgc taaagcagta gagacaaata
    41461 aagcattccc gacaccccag ccggcacgta acccaatcag cattccgata ttcccagata
    41521 atccgccaag accagcgaat acaacgatga taaacaatcc tgtcatcaat gttttttcaa
    41581 accgatccga ctagataaga aacctgtaaa tagcatcacc acaccagtga ctaacatgta
    41641 actagtaaat aacagggtcg tttcagctgg cgtagcgttt aactgtgcag caatcgtttt
    41701 taagattggg tctactagac ccactcccat aaaagcgatc atacaggtag ctgctacaac
    41761 tagcgctgaa cttttcccct ttgtttcttc atttgtttgt tcttgtatat ctatttgatt
    41821 ctccaatatt tccatccctt tctctctatt agcatcatac atgatttatt ttacatgtaa
    41881 atatgcacat gtaaaacgtg gaaactttac tgtgatataa taaagaaaat aagacgagag
    41941 gtaggatttt tatgaatact ttagcctata tattattatg cgtgctttta tccaaaccac
    42001 gttctgggta tgaattgaag cagctgatca caattttttg ggaagcccat cacagtcaga
    42061 tctatacaac gcttgccaag ttagaccagc agggttatgt gaccgttttg gacaaagacg
    42121 agcactctca aaaaaagatc taccacttga ctgatgaagg acgtgctctt gtagaggaat
    42181 ggatcaaaga ggaaactccc gctcctacgc aaaaagatga atttctagca aaaatctatg
    42241 ctttttcgac gctcgataaa acaacagcta ctggactgct attcactaga gaacaacagc
    42301 tgaacaaaat catgcaaaaa aatcaaaaaa aattgaatga cctctcgcta gacaagaaac
    42361 aagaatttgg acgttatgtt gtgatccagc gcagaattat gctttgtcag caggaactcc
    42421 aatggtgtca gcaagtaaga gaacaaatgc agcagttttt tgagtaaggg ataaacagaa
    42481 cctttctaga aatatctatt gaagagcaaa ttatcttcaa taaaagagca atacagttgg
    42541 aaaagaaaaa gcgatccgaa cgacaggcca aaacctatcg ttcggatcac tatttttcta
    42601 gaactgcatt caagagacta tgacaaaaag catgccaagg ccttttttca aataacagtg
    42661 ttccatcatt cattactttg aattgctttt agcacacttc ttgaaagcgg gccaacgatg
    42721 atcaactgca acggtaaagc ggcaatcaca ttcattttcc aagcattcat ataagcagag
    42781 aataattgat caggtattcc ttgttccatg atcacaccga ataaagacat acatgtcacc
    42841 attcctaaaa ccatcaagca tgaaatagtt aagatcattt ggaccttgct ttctggattt
    42901 actggtagtt taaaggcaat ttttttcgcc agcacaccga tgatgaagac atccaaaatg
    42961 aaagccacca cgaatccagg cactagacca atcaacaaac ttgataaatg aagatcttga
    43021 tgcagccata aattatacgc gctcatcccc aagaccatca aaaaacacat cattgttgta
    43081 aaaattattc cttctttttt atttgtaggc aatctgtacc actcctttaa atttgtcacg
    43141 aaaatcatcg tagcacattt aaaaatttct cgtaagtact ttttttattt tctgcccttt
    43201 tatttcataa acttcatcag ccgtacgctc gacaaagtaa gaatcatgag aagtaaacaa
    43261 aatcgttccc ggatatattt gcattagctt ctctaaagct tcgatcgttg ccatatcaat
    43321 aaaattagtc ggttcatcta acagcaacac attacttggc ttagtaaaaa gcaaagcaat
    43381 cgtcagacgg gtcgcttctc ccccacttaa tgtacataac ggtttcgtca aagtttcctg
    43441 agcaaaccct aagtgattca gcaaactacg gacaagcgat tctgaaaact ctgtttgtct
    43501 catgacaaaa cgaatgatgg attcttcaga tgtcatttga taaccctttt gctgatatac
    43561 ttgaaagctt actttcggag agagaaggat tccttctcct tgtttgcgga tatgttctaa
    43621 taaggttgtc ttacctgaac cattttcacc gacaatcgcg atttttttac ctaaagaaaa
    43681 ctgaaaatca ccatttacca gtaatgttct cccacttctt tccaattgga cattttgtcc
    43741 catgatggga tagcggctgt ggagttcaag agattttggt actggaaaac ggatttgttt
    43801 catctgctgc ggcttggttg tttctcctat tttttggagt cttctctcta atgttttcgc
    43861 attcttttgg atggcttttt gtacactgtc tttttgttta gaggaagaca accgatccgg
    43921 tctgatttgt tgctttttct ttttactgga cactttttcc aacttttccg cttgtttttt
    43981 cttttcttgg atcgattttt ccaatcgttt tttctctttc atgatcgact ctgcttctcg
    44041 tagctgagtc tttttctcca attccttttg agtgagatag gcactataat tccccgaaaa
    44101 ctctcggatt tctccgtctt ttacttccca gattttcgat gctagctcat ctagaaaata
    44161 gcgatcatga ctaacaaaaa ccagcgtacc ataataataa cgaagctctt caatcagtcg
    44221 atcaatactt tttctatcta gatgggttgt tggctcgtct agaagcatcc cttgttcata
    44281 agaagaaaga agacgtatca actcgatctt tcgttcttcc cctccgctcc atttccgctc
    44341 tgaagtttct tccagtctta actggaaatg actggctaat tccaaattta ggtcctttgc
    44401 ttcagcgaga taggttaatt gaggcaggta gttgaaggtg atctctgttt ggattttccc
    44461 atgatcggga aaaagttcac cggctattag tttgagcaaa gtcgatttac cgacaccatt
    44521 ttctcctatg atccctatac gatcttgctg atagacacga agagaatcaa tagatagttc
    44581 tagctggttg ccaaagctga cttgtagatt tgttatattt actgctaaat tttccatatg
    44641 ttttacactc ctatttcatg taaaacaaca attatcgata ctaaaaaaga gcagaaaaat
    44701 ctgtttgcaa aaaaactgca tcacatcttt tcctactctg aacgaaataa ctatgactaa
    44761 atcaccaaat ctgtcatagc tttcacctat tctactgtcg ttcgaaaaaa tggacatact
    44821 ccatccatga aattgttgtt tatacatgtc ttttgaattt atttagttgt tattcaaaag
    44881 ttcgaaacgt aatttcatcg atgcagtcat ttttccgacc tcctttttta ttaggttacc
    44941 acacacgatt caaaaaaagc aatagctact gaatagtaac tgaaaacctt ttattcgaac
    45001 gtagtgatct tttctggtgc taaacgaaca gactcgtaac cttcttctac cgcttttcct
    45061 attgataaaa taataactgg aacataacgg tctttatcca aaccaaatgc ctctgccaac
    45121 tgatctgctt cgaatccacc aattggattt gtttcatagc catgatcacg cgcaactaac
    45181 atgaattgca tagccgctaa gctagaatcg actttgacta catcgttcat ttcttctctt
    45241 gaaaaactct tgtaataagg aatgatcgca gctaattgtt gatctctgac ttcttttggc
    45301 atcttccctg attcgtaagc ttggttatag atttcttcgc catattcgta acattgcata
    45361 tcgccaaaaa tcatgaccat tgcagaagaa gtatcatttt gtctcgtatt gaaacggatc
    45421 aaaggacgca agatttcttt tgcttcctcg ctttcagcga ccacaaagcg ccatggttgc
    45481 atattgacag aagaaggtgc agtcgtcgct tctttgatca tttccagcat ttcttcgtgt
    45541 gggattttgt atgtttcatc atatacgcga acggatcttc taccagtcat aattgttgaa
    45601 aaatcatttt tttgttccat tattattaac ctctttcgtt tctgttgtta tattgcaaag
    45661 ttgggtattc aatttatcta aaagtgttag aaattgtttc atttctacat cgctgaaagc
    45721 cggggatagc acggaacctg tatctttatg gtccatttca caatgctcga ttttcttctg
    45781 ggctttttct gttagcgcta catacacttc ccggttgttc tgaggatttc tctgacgcga
    45841 aacatagcca tttgcttcca ataccttcag atgacgagta actgctcctt gatcaatctg
    45901 caacttttct tgcaaactgc tttgtagcaa tggattttct tctttcagtc tcatcatgat
    45961 ctcgtatcta gtaagactta taccgatatt ttcttcaaag atctggacga ttttttgatc
    46021 ggctaatttg atttgatata aaaggtgact aatctcttct aaagacataa tttcctcctt
    46081 tctaataatt gactcgtcaa cgattgactt gtcaataata gaataaaaaa agaaaaaagt
    46141 caaagaatca gctgtgtaat aaaaaaaacg ctctcaatag aaaagtatag agaaaagaaa
    46201 ttttttgtag aatgaacgga aaaaatgata aagaaagcac aacgctaaaa ccaagattat
    46261 tgattcttat caaactatag aagatagctc ttctgcagta gaaaatttta caccgccaaa
    46321 aatttccgaa atcgcctttt gcaagaatcc cgtcgaatat tagaaattgg atcagtatgc
    46381 ttaaacagta ataaaaaagt tatgacaaga tgttgtcaca accttttttg agtaaacggt
    46441 gttcaaaaac ttgcatctgc ggtaataagt tcaaccaaac aaaaaacatg aaaatctgtt
    46501 tttgtttggc tgttcttact acttgatgca gggcagcttt ttcactacct ctttttaata
    46561 accataaaat cctattcctt catatctcca gcccatcggt gttagccaat ctctttcctt
    46621 agcactcaag gtatagtgat gggtaccagt tttttcattt ggattaaata gtcgataaat
    46681 tggcttggaa ccaccagaat aaaaacttag accttcataa cgccaaccga tttttgttaa
    46741 agagttgatc tcagattgtg ccattgtgta atgatggtct ccactgtaag gattgtataa
    46801 acggtagaca ggttgtccgc tgtttggtgc ttgccaagca atcccttcat attcccagcc
    46861 tacagttggc agataatttt tttctccggc attcaatgta tataaatgtt ctcctgtatg
    46921 acggttatac agacgataca tcggagaagt tgaagttggt gctgtatcat actgtgtcag
    46981 attataggtt tcgattacat tgttcaatgt agaaccataa ttaggcgcag tggcataacg
    47041 acctgttagc caagccgtag catctttgta agagttcgta ttgcttttcc atgcaccgga
    47101 atagtagtaa acgcctggct gaaaagaagt cgtcttcaat accgttacgt tatcttgcaa
    47161 tgattctttg tacgaaggat attttctaaa agcggcattc accgtaatcc attgcccatt
    47221 cacatattcc gaagtaggca tcgtaacaga ttggccatta tagcttcctt taatcccaaa
    47281 tagattgtaa ttaggggcag aagccaaagt actgttcccc cagccacttt caacgatggc
    47341 ttgagcaatc atgactgatg catataagtc attggcactt gccaaacttt gtgcagttga
    47401 ggcaatacta tcaatgaacg cttgttgaga attagcagat gagcgagcat aaaaagcata
    47461 gccagtatag ttttcgaatt cagacaaatc tgcttgatgg gtatgtgtca atgtttgccc
    47521 atctgtcaat tggttcgtga tttcttctgc attctctgga ttgttctgag cttctgctga
    47581 tgtactgtct tcggtttgtg tttgttctgt tgtttcagtt gttggttctt cggttgaaat
    47641 tgtattttct gagacagtag ctgactgcaa agtactctct gtatcgtttt caattcctgt
    47701 ttctgaagaa ctcgatgtct gagttgcgac agtttctgtc gattcttcag ctagcactgt
    47761 tgtgttggca actgctagat ttcctaaaag aataacgcta aataataaat tctttgcttt
    47821 catctttttt ctcctttgaa ttatgaaact tttcctaaat aacagtgtaa ctaaaaaagt
    47881 aaaagcaagt caatgtccat atttaaataa catttgtttc tttttgataa atttgctttt
    47941 tctacataaa aagacgtaaa tgaagaatat tcttctccat tcacgcctta aattgatggt
    48001 cgtttgaata actatttaac aagtaattct ctttggatat tatccgttat ggattatact
    48061 tcctgtgtcg tcggacggat aatcatgtta ctgacagaca tccggtctgg cgtttctatc
    48121 gcaaatgcta cagcagaagc tatatcttcg gaggtcaatc cccattcttt ttgcgcttca
    48181 tgaagttcaa gagcagcctt tttgtcgctg atggtttgat ataattcagt ttgaacagct
    48241 cctggagaga tgatcgtaga tttgatgtta ttttgccgtt gctcttgacg taatccttcc
    48301 atgattgcac ggacagcaaa ttttgttccg caataaacgg cggaatcagg ataaacaaca
    48361 tgtcccgcca cagaatcagt tgaaatgata tgcccagatt tttgtttttc catgattgga
    48421 agtacggcgg aaattccatt taacacaccc atgatgttga tatcaagcat attttgccat
    48481 tcatcacggt gaccttcaac taatggagcg gttggcatga ttcctgcatt attgtacaat
    48541 acatcgattc gaccgaactt ttccatcgct aaatcgataa ctgcttggac ttgtgcaaaa
    48601 tctcttacat ctgctgtttg aatatatagc tcagcttctg gtaaagattc ttttattgct
    48661 tttaggcgat cttctcggcg tgcagctata actagtttag cccctttttc tgctaatagc
    48721 ttcgttgttg cttcaccgat tccgcttgaa gcgcccatga tgacaatcac tttttctgat
    48781 aaatcattca ttctattctc ctcctgtttt gaaaaatctc ttgcttatac tcactatctc
    48841 ttatttcatc ttactgttca aataatccgt tcgacaggtt tgacgatttt ggcagggatt
    48901 cctgcaacga tggtattgtc tgggacatct ttcgtaacag ttgcattcgc tgcaacaatg
    48961 gcattctcgc caatcgttac gcctggcaga atagttgctc ctgctccgat ccaagcattt
    49021 ttcttgatgt ggattggctt tactgacaag cctcttcgtt cttttggatc ggttagatgg
    49081 ttcaccgtga tgaggttgac acgaggacca atcaatactg aatcttctaa acagatgcca
    49141 cctaagtcaa cgaacatcac ttgacgattg ataaagacat ttttccctat actaatatgt
    49201 ttgccgaaat ctgtataaaa aggcagcaga acagtcaccg tatcatctac tttgtcacta
    49261 atgatatttt ctagccgttt gcggatatct gctggttcat ggtacccagt attcagcgct
    49321 gctaaaggct cttcgttttc agcttggatc tgatggatca cttcaaagag cggatccgtt
    49381 actcgaatct ctttattgac gatttgtttt tcgatgaggc ttgtcatttt gctccctcct
    49441 tttatcctta ctttataacg gacggcttat catgtctaat acttataaaa catacttaac
    49501 tattcctttt aactatactt agtggtatgc ttttttaaaa acaggaggga aaaaatggat
    49561 attcgtatac tgacgtattt tttagcagta gctagagaaa aaaatatcag ccgagcagca
    49621 aaatctctgc atatcacgca acctactctc tcaaaacaac tgaaagattt agaagaagaa
    49681 cttggtgtga cacttttcac tcggggctcc cgagagatac agttgacgga ttctggtcga
    49741 tatctttatt caaaaggaaa agaaatcttg tcgttggtag aaaaaacggc gaacaattta
    49801 acaaatgacg caatcatctc tggagaaatc tacattggcg gaggcgaaac taaagcaatc
    49861 tctttcattt ctgatagcct tcattcgttg atcaaggcgc atcctgatat ccagtttcat
    49921 ttatatagcg ggaatgcaga tgatatcata gacaaactgg atagcggatt acttgatttt
    49981 ggactcgtaa tcgaaccgac aaacaaacaa aaataccagt catttcgact gccagcccaa
    50041 gatcgttggg gacttttgat acatcgaaat catcctttag caaagcaaac cgtcatccat
    50101 ccgcaggaac tgaaaactgt tcctttgatc atttcccggc aaacgtcagt ggacagtcaa
    50161 ctagctgaat ggctaggttt cagcatagat cagttatcga tcgtcggaac atataactta
    50221 ttgtacaatg cttctttgat ggtcgataac gggacttttg gtgcattagc tctagatggg
    50281 atcatcaata cttatgggac ggaaaaaata ttcatcccac tcgatccgcc attacaagct
    50341 tcactgaatc tcatttggaa aaaagaccag cctctttctc ctgctgctcg taaattttta
    50401 gatgtcctaa cggagaaata cagttaagta gagttttttt ccttcgcttt cagcttttta
    50461 acaacgaata agataagcaa aagatccaac gattaaataa cataagtttc ttaatttatt
    50521 tcttcctttt atttctgata attccagtaa attactataa ttataaaaaa agaattgaca
    50581 aaacataact gtctatataa aattgattac gttaacaata attcgtttca tgtgactaga
    50641 tcatactagg catgaaaaat ctctgaggaa catggtttcc ctaggaggtt tttcgtgtct
    50701 atttttgttt ggccgcaatc aactggtcta gcaaacgtga aaaccaaatc ttcaaaaagg
    50761 gtgcgcgcat gaaaatcaaa aagatcttta atcaaaatgc agtattggta gatgacggtg
    50821 gacaggaaaa agtagctatt ggaaaaggga ttggatttga cagaaaaaga aatgatttga
    50881 tttttgatcg tgatgtcgaa cggatatttg tcatggaacc agaaggtcaa atcaagttgc
    50941 aaaatttact taatcagatt gatgaacgct ttttatttgc agcagaaacg atcattgatc
    51001 atgcggaaac tgttttgatg gaaaagttga atgaacatgt attgatcgct ttgaccgacc
    51061 acattgcttt ttctgcggag aacattaata acggtatcgt catccgcaac aaacttttaa
    51121 gagaaatcga agtgctttac agtgatgaat tcagtattgc tcaatgggca gtagagtatc
    51181 tgacaaaaga gcttgggatt ccatatactt atgacgaagc aggatatatc gcaatccata
    51241 ttcacagcgg acgaagcggt cggaacaata accatcgcag tatccgtgaa gtgacgattg
    51301 tatctgacat catccattta gtggaatcag aattggaaac agatatccat tcagaaccat
    51361 tttcactgaa ttattcaaga ctggtgaatc atttacgact gcttctacaa cgaacccatg
    51421 cgcaacaata tgctgtttta gacacggaaa tcgtagaaat ggtcaaacga aaatacccag
    51481 aaagctataa gatttctaaa aaaatccgtg tgttgctgac caaagaatac cagctcgcga
    51541 tcacgaaaga agaactgggt tacttggcca tccatatcga gcgtttaaga agcgcaatcg
    51601 tacaaacaaa tgttaacaat catgaggagg aaaaaaattg aaagcatatc tacaacgaat
    51661 gggacgttcc cttcaacttc cagttgcggt cttacctgct gctgctttac tagtagggat
    51721 cggaaactgg tgggcatctt atagtaacga cattgtcgct cattttttac aagctggcgg
    51781 taacgcagtt ttaggtcaat tacctttgct gtttgccgtt gggttagcac tagggatgtc
    51841 agtggacaaa tcaggtgctg ctgccttggc aggtttggtc gcatttgaag ttccagtaaa
    51901 tgttttaaaa actgattcag tagctacttt attgaatatc aaaccagaat cagttgatcc
    51961 atcttttgct cagatcagta acgtatttat tggtattatt gccggattga ttgcggcagc
    52021 tctttataat cgtttccacg aagcaaaatt accgatggct ttatctttct ttagcggcaa
    52081 gcgtctagtg ccaatcttgg ctgcctttgt catgttgctg atcagtgcag tgctactgct
    52141 cgtatggcct ccggtttact ctgctttagt ttcttttgct aaattcattt tgagtttagg
    52201 ctggggtggt gcaggtcttt atggcttctt caaccgttta ctgatcccta caggattgca
    52261 tcaagcaatg aactccgtgt tctggtttga cgtagcagga atcaatgata ttggtaaatt
    52321 tttggcttct gaaggaacaa aaggcgtaac aggaatgtat caagctggat ttttcccaat
    52381 catgatgttc gggcttccag caggagcata tgctatttac agaaatgcac gtccagaaaa
    52441 caaagcaaaa acagcttcct tgatgctagc agcagctttt gcttcattct ttactggtgt
    52501 gacagaacca cttgaatttt catttatgtt cgttgcttgg ccattatatg tcttgcatgc
    52561 aattttcact gggatttctt tagcagtgag tgcattcttc cattggacag caggatttgc
    52621 ctttagtgcc ggatttgtcg attacttcct atctttgaaa aatccgattg cgaatcagcc
    52681 attgatgttg atcgtccaag gattagtaac agcagtgatt tactactttg gctttaactt
    52741 tgctatcaaa aaattccatc tgatgactcc tggccgcgag gaagcagatc tttcagatga
    52801 cgatactgca acgactaatt cagacaataa atatgcggca caagcaagca aaatctacgc
    52861 tgcattaggc ggagccgaca atgtaacttc gatcgataac tgtactacac gccttcgttt
    52921 gcaagtcaaa gatacaggcc tgatcgatca aaataaaatc aaagccactg gcgtacctgg
    52981 tatgaagatc atcgacggaa aaaatgcgca agtcatcgta ggtacagaag tacaattcgt
    53041 tgcagatgag atggcaaaac tacatggtgg tgctgcagca cgacctgcac aaacaaacac
    53101 cgtggttaaa acagaaacat ttgccggtga aacgatcgaa caagatattt acgctatcgc
    53161 aaatgggaaa ctgattccaa tcactgaagt ttcagatgat gtattcgcgg aaaaaatgat
    53221 gggtgacggc tatgctgtat taccagaaaa tggagaaatc ttctctccaa tcgctggtac
    53281 gatcacaaac attttcccaa cgaaacatgc tgtcggaatc caaacagatg ctggtatcga
    53341 agtcttgctt catatgggca tcaacactgt tgacttaaaa ggcgaaccat tcactttata
    53401 tgtagaagaa ggtcaaaaag tcgcaagagg tcaattgatt gctttagttg atcttgccgc
    53461 tattcaatct gcaggcaaaa acacggatat ggtcgttgta ttcacaaatg gcgacaaagt
    53521 ccaaagctta gaaatcgaac ccgcacgtga tgtaaaagca aatgataaaa tcggctctgt
    53581 ttccaacaaa gcctgagcca tcgtcttccc cagacttttt tatcgataaa caaacaggag
    53641 ctgagacaaa aatcttgtct cagctccttt ttccgaataa aaacggcgtt ctcctgccaa
    53701 ctccgcggta ataagtccag ctaaacgaaa catatgaaga gctatttttt atttgactct
    53761 tcttattact tgatgcagaa cagctttttc acaaaatttt tcttcatact caaaactaat
    53821 cggctttttc acaacctctt tatacattat tcctgatcca atatttcttt taatgctgct
    53881 tcaagagaag ttaatggttt tcccaatact ttttctaaat catcagagga aatagctaac
    53941 tgatcctttt taatatcatg ttggatcgct aaaaacatgt cagctgcttc ttgagggaat
    54001 ccggcttctt ttagattttc gacaaacgct gcatcacttg cttcaataat ttctaactct
    54061 ttacctgtcg cacgctctaa tgccttggct aattcttcat agtcagttag attgccggat
    54121 aattctaaga tttctggaga gtcactttct actagtactt ttgctgcagc ttctgcatat
    54181 tcacgtttca atgcccaacc tgtttttcca tttcctgcag cataaacgaa ttgaccagtt
    54241 tttaaagcct gcccaatgat aggtaattca ttctctacat accaattatt ccgtaaaaat
    54301 gtatgggaaa tacctgaatc cttaatcatt ttttcagtga attggtgatc tggcgctaat
    54361 acactgatcg agtgatcggc atctgcaaaa cttgtataag cgatataaga taccccagct
    54421 gcttgtgctg ccttcactac attcttatgt tcggcttgac gatttcctgg aacgccagag
    54481 acaaaaagca atcgatctat tcctttcaac gcttctttca ttgagtctaa atcatcataa
    54541 tcaccaatac gtatattaaa tccagcttct ttcagtgctt ctcctttttc ttcactacgt
    54601 actagtacat agatttcatt ttgtggaacc atctttttta agaattccag cgcataacca
    54661 ccaaaacctc cagttgctcc tgttactaaa taatgcatat ttgccactcc ctattctttt
    54721 tatttgttaa gatttacaga tgtacattta taaattacac ctttatcata aagagtgtgg
    54781 taagataagt caagcaaaaa aaatgaaagg atgacaactg atgaagtatt ctctgcaatt
    54841 tagtgatgca atccatgtac ttgcctatat tgaaatgttt caagataccc gatcactttc
    54901 tagtgagatg attgccagta gtatcgaaac aaatgcagca aatgtacgta aaatcatgag
    54961 tcagctcaaa aaatccggtt tgatctttac aaaaaccgga aaaccgcagc cttcccttgc
    55021 caaaagtcca gatcagattt ctttactaga tgtctatcgc agcatcgaag gtaatacaca
    55081 gcttatccaa gtcgatccaa aaacaaatcc aaattgcgtc gttggtgcga atatccaaga
    55141 agctttagaa gaaagctacg ctcggctgca gaaaaaggca gaagaagaaa tggctaagat
    55201 cacacttgat agtctgatac ataaaatagc cgttttggaa aaagaaaaaa gaccagaaaa
    55261 cacaggcctc gtagagagat ttttataatt ctctctactg tgcctgcatt tttctgatcg
    55321 gaatcgtcta cataaattct tttattctcc gggggacaaa agccttgtca tgcccctttt
    55381 tcctctctaa tgctcaggtc aaagcgctga agtcatgacg tcttgataaa cggtgttcaa
    55441 gtagctgacc ctcggcatta gttctgaaaa atgcaaaata tggaaagcta tttcccattt
    55501 ttcctctcta atgctcaggt caaagcgcta ctttcacgac ctctatatta tcatggttta
    55561 ttgactaccg gtatccagat ttctcccata tatttcccgt tggtaactcc catttcgact
    55621 acagcatttg gacccccaac ataggcgtaa tcttctactt ctcttaaaac atgcccaaag
    55681 gccgtattcg tcaaatcatt tcttaattct tcaggcgatt ctgctaaagc gcttacaaca
    55741 agatacgttc cttttgggaa tgggatgatt cgcgtagtgt ttggaacatc ctgactcgtt
    55801 tgaacaccga catagtacat cattttcccg ttatagggtt cgttgactgt aaagaaataa
    55861 tgattttcag cagcttgttt caatgcatct attgttccat taccgataac tttttgctca
    55921 aatgcttgtt tctctgcagc caaaccgaca aaatccgtat tgctactctt caattccaca
    55981 ccaatacccg tcaccgtaaa atctggtttt tcttcgatcc tatactttgc catttctccc
    56041 accctttcta ttatattaag ttcatcttac ataaaattaa agggaaatga cacgattacg
    56101 ctcttttttc tagggatatt tttatttttt aactattttt ttgctttcac tgcaatccaa
    56161 atctcactgt aaacattact gaaatcctct ccccattttg taaaagagat ctcgggagct
    56221 tcgactaacc cataatctga agaaggaagc cattctgcat agattttccc ccaagtatct
    56281 tgtaaagttt ggggaaatgg ccctttatta ggaaaaatgg cccaagtgtg ttcttctata
    56341 gcaagcattt ctaaatcagc gtacggattt tcttttgtcg tagcaaagcc aatcatgtgc
    56401 atcaaactgc ccttttcttc caagcggtct ccatcaaatc cataagaaac gttgattact
    56461 tgatttggat ataaatctcc taacttatgc atctcttccc tttgagtctc cgtgatcgtc
    56521 tgcgctaact tttgtatttc ttgattgact ccttcaaatt tgacaggaac ttttttcgcc
    56581 acacctgcca cataaaactg ttctttcttt tcgatacgaa attccatcga ttttcctcct
    56641 ctgacatcaa taaaaaatgt caagcgtgga aaagattttt gtatgccgta tctactgatt
    56701 tctgaaggca gatatcctga ccaatcacga aaagctcttg aaaaaccttc gactgtttca
    56761 tatccatatc taaacgccgt ttctgtcact cttgatccat ctagaagatc gcgattagcc
    56821 aaagccaatc tacggttttt gatatagtcc attagcgaca tccctgcaat aaacgtaaag
    56881 atgcgtttta attgatattc agagattcct gtattttcgg ctattatttt ataggaaagt
    56941 ccttctgtca aatgttcttc gatgtaatcg atcaattggt tcagatcttt aagcatattt
    57001 atcttcctcc ttgattgcct ttttatcata ttatcaaaga aagtcaatta cttgatgaat
    57061 caggaaccaa tttagtcaga tgtagaaaat gttgtatgga tctaaagaaa attctcaagc
    57121 agcgtttcgt aacggataat tggtattatc tgcctatcta tgttttacct ttagaaaaat
    57181 aaattttggg gaagacagat tctaagtaaa caaagcggta aaaatatctt ttattccctt
    57241 agttaatccg cttactacct actgtacaaa gcaaattgtt gttcaaactg ctgatcagaa
    57301 cgctgcttgg atttttcttt gcgatacatc atacctcttt tatcaattgg aacaattatt
    57361 tatttctaac aaaatcactt catttttgtc agataaataa aaaacaaaaa aagttccatc
    57421 tttaaaacac gctattcttt tactttttct cacattctct aagaaaagaa aaggagaatg
    57481 aatttttgac aattcgagca aaagtgatta tcaaatatgt aaaatcagct acttttttct
    57541 taaacgtttc tttatactaa atataaataa gtgtttcatg aaaggagaac tagatgaata
    57601 attggcttga gtattttcct gaaaacgttt tggaacgcgg ttacagctat catctgcacg
    57661 gttttgtccg tcatttgaac tatacaagca agtacttgtc cgcaacagtt tctggcacgg
    57721 aagattataa ggtagtgatc acttgggatg aaaagaccaa catgacttgt gattgtctat
    57781 atgccataga aggaaaaaaa tgcaaacaca tggctgctgt cttattcgct tacgaagaac
    57841 gtccaataaa aaaatccaac tactcacttt ccgaactctc gtcccttgtt tcctccgcta
    57901 gttcctctct tgtacgggag cttttaacag agattcttat agaacatccg cactttatcg
    57961 aacgcttcaa agttaagatg ccatttcatg caataaatta ttcagataaa ttaacaacta
    58021 tcatccataa atacgatcat attatcaaga aaaacaaaaa caggaaaaca gcgaagttca
    58081 tcatggagat gcgaaagttc atccaagaag cagttgaatc gcttattcag caaaatgcct
    58141 atcttcccgc attcgaattg atcaatgaag tgattgctac tctcgaaaca ttctattggg
    58201 agcctgagga tgaacgaacc cttcttctga tagaggactg ttattattta tggaaagaat
    58261 tattagcaga agcacctcat gcagaaaaaa ggcaaatgtt ctcttggttc gtctgccaag
    58321 tcgatcatac tgacgcctct tattcaaaac gctactctat aaaaatcctg aaagaagact
    58381 ttcgggaaaa agaattttct aaccaaaaga aaaaaataga taaaaaacta aagaaacggt
    58441 aaagaaagac gatttcaatg aaaaatagca agtaaacgat aacaaccatc ctaaatccac
    58501 aagaaataca gcagaccttg ctttaaaaac acgaatcatg caaaggatga agtaaagaag
    58561 acttaacgcg tacccaatat tagtcatttg actattaggt acgcgttttg ttttataagt
    58621 catgacaaat ttccttcgtt gctggataga ttttccggca ttgttgatag attttatgat
    58681 acttttctac attttgtaag agaggttccg ctgcatctgt atattggata aaattctgac
    58741 aacaactttc aggtgtgtcg aaccacccta accctagtgc agcaatcatt gttgcgccta
    58801 atgcaggtcc ttcctccata cttaaacaat agaccgttgc atcgaaaata tcagcttgga
    58861 tttgtagcca tgcaagattt ttcgcgcctc ctcccaccga aacaatacgt gtaaatctgc
    58921 gacccgctag ttctttacgt ttcaatccca ccattgacaa atgctcatta aaaatttaga
    58981 atattcacaa gtttaacgac aattatgaaa atttatgtcg tgctaaagga gttttgttat
    59041 tggatgaaaa gttagatata aaaactgcga tcaaagacac gttacctacc gttttcggtt
    59101 atatcggtat tggacttgca tttggtatcg ttgggaaagc tgccggattt catccactag
    59161 tcgtcacgtt gatgtccctg ctggtctatg ctggttctgc ccaatttatc acagtcagca
    59221 tgcttgctag tcacagccca ttgctttcca tcgttttctc gacctttcta gtcaattccc
    59281 gaatgattct gatgagtatg acgattgctc cttatttcaa gaaaaatcgg ctgcttcaaa
    59341 atctgctgat tggcacattg ctgacagatg aaagtttcgc actgggaatg aataaattga
    59401 actatactgg acaaaagtta aactttcgtt ggatgaatac agctaattgg atttcttatt
    59461 tgacgtgggt gggttcttct cttgttggcg cacttttagg gaattttatt actgatccga
    59521 aaaagtttgg tttggatttc gccatcgtcg ctatgtttat cgggttactt tatcttcaag
    59581 tcatttctga tagaaacacc tctaaacgat tacagttgat cctgattggt ctcactttga
    59641 ttctggtata tgtcggactg atttttatcc caagcaactt agtgattgta gttgtaacac
    59701 tgattgggtg cggattgggg gtgtggatca aacatgcctt cttttgagtt tacattgctg
    59761 actattttag gttgtacttt agcaacttgg ctttctaggg tcctaccatt tgtcttactg
    59821 aaaaaattcg atttaccgca accgttgctt gagtatttaa gttttgtccc gatcgtcatt
    59881 atgtctgctt tatggttcag cagtctattt acccaaaata tcggacatct gccgcagatc
    59941 aatctcgaaa atgctttggc ttcagttcct acacttttag ctgctattct ttcaaaaagc
    60001 ttattggtga tcgtcttggc aggaattctt tccctttcgt tgattcgctt attataaata
    60061 aaaaaacaag agcttatggc aaattactgt catgcgctct tgtttttatc aaagtcaaat
    60121 aagaaaaaaa tcggcgtatt cttgcttatc cgttgaacgg accactaaca acgacttttc
    60181 ctaacatatg tcctgtttct acgtctttgg ttgcttgttt caagttcttc gcattcatcc
    60241 catctgaata gaccttgctc aatgtactgc ggataatccc ttcatttgct aaagcagcaa
    60301 tgtgcgctaa agcttctcct tgcgtttcga tttcatagtt atagtctgtt ttagcgaaca
    60361 tatactccca gtcgaagctg atagattggt ttttccaaac acctatatct aacggctctt
    60421 tgatccctac gatcgttccc acatgtccaa atggtcggac aaggtgcttg atttgatcaa
    60481 agtagcgggt aatatcgaat aatacagcga catactctac ttgatcgatt cctaattcac
    60541 gaaaactttt acttaagtcg tgatgataat cgattggata gtctacacct gtttttttca
    60601 gccattcaaa attcttcgga cttgcagtcg cgtagacttc taggccagcc caatgcgcta
    60661 gttgattcaa aatcgaaccg acgcccccgg atccgttgat cactaagatt tttttcccgc
    60721 gattggcatt ttcttctgga gttagaccaa atttttcaaa taacaattca taagcggtca
    60781 atgaggtcaa cggcaaggct gcagcttctt catctgaaag attttttggt gcaagagcga
    60841 caatacgttc gtcaactagt tggtattctt gattgcttcc tgctcttgtt gttgtacctg
    60901 cgtaaaaaac gcgatcgccg acagaaaatt tttgcacttc gtcacctaca gcaacaactt
    60961 tcccgactcc atcaaaacct aagatacgta aagcatttct tatcccgtta tcttggcgaa
    61021 gtttcgtatc gacaggatta acagatactg cgttgacttt taccagaaga tcccgtgaac
    61081 tgggtacagg tatctgttct ttttcatcaa taaagctttt tggatcatcg atcggcaacc
    61141 cttcataaaa accgactacc ttcattaatt ccttttctga catacgaatt cctccttagc
    61201 ttcatcaagt tggagcatcc atacagtgtt catttcaata tatatagtat gctatttgtt
    61261 ccaccttttt tattcatcat aattattctc ttacttaaag taaactattt gcactttttt
    61321 ggttaaaaaa gaggttgcga caagactttt gtcacaacct cttataaacg gtgttctcct
    61381 accaactctt cggtcacaag tcaaaatatt catcaaacat gagaagctgt tttttgaaat
    61441 tgctccttgt gactcagagt taaacggcag gctcacaacc tctgatataa acggtgttca
    61501 atcaactgac cctcggtatt agctcaaaaa acgacaaaat atgaagagct atttttgttt
    61561 ggctgttctt attacttgat acaggacagc tttttcacga cctctaattc acggtgttca
    61621 agcaagaaat cattgttcac tgaaaagcgt tggatgttct ttttcaaagg tctctaagat
    61681 ctctcgtact tcatcagtag atttcgtatt catcaactga actcgcaagt catttgctcc
    61741 tggaaatcct ttgatataga ttttaaagaa acgatgtagt cctgtgatgg aacgcgggat
    61801 gatttctgaa taatgatctt gcagatctag ttgcagacga agtagtccga tcagttcttt
    61861 ttctgtatgt tccctcgggt ctttttcaaa tgcataagga ttcttgaaaa tccctcggcc
    61921 aatcatgatg ccatcaacgc catatttctc ggccaattct tctcctgtct ggcgatcggg
    61981 gatatctcca ttgatcgtga ttagtgtttg tggagcgatt tggtcacgta aagcaatgat
    62041 tttaggaatg acttcccaat gtgcatctac tttactcatc tctttacgcg tacgcaaatg
    62101 gacagaaaga ttggctatat cttgatgaag taggtgactg atccattctt ccatctcaga
    62161 catttccgta tagccgatac gcgtcttgac gctgacaggc aaaccgccag ctttagctgc
    62221 ttcaatcaat tgtgccgcta cttctggacg aaggatcaag ccactgcctt ttccacgttc
    62281 tgctacatta gggactgggc agcccatgtt gatatcgatt ccttggaagc ccatttctgc
    62341 catagcaatg ctcatttctc taaaaaattc tggcttgtct ccccaaatat gggcaaccat
    62401 cggctgttca tcttctgtaa atgttaatct tcctcgtaca ctgtcttttc catctggatg
    62461 acagaaactg tctgagtttg taaactcagt aaagaagaca tccggtgctg ctgcatgctt
    62521 tacgacatgg cgaaatacaa catctgttac atcttccatt ggtgctaaaa caaaaaaagg
    62581 ctttggcagc tctttccaaa aattagtagt catttatttg ttactctcca ttctatcaag
    62641 cagtgaagtc gcaatctttc tcaaaaacat tgtcattata ctaggaaaca aaaaaagaag
    62701 caaagtgaac agcttaaaag atgtttctga ttgagcaaaa aaaagactgt gacaaaaatc
    62761 ttatcacagt ccattttccg gaataaatgg tgttcaaaag cttgcatttg cggtaataag
    62821 tccaaccaaa cgaaaaatag gaggagctat ttttgtttgg ctgttcttat tacttgatgc
    62881 agaacagctt tttcacaacc tctccactaa acggtgttca atcagctgac cctcggtatt
    62941 cgctcaaaaa acaacaaaat atgaggaact attttcccgt ttgtttctct aatactcagg
    63001 tcaaaacgct gatttcacaa cctcttttct aacttctcaa tcctcttcct ttattgatca
    63061 aataccaagt aacggcgtac aaaacaacga tgaataacac aagtacgatc atcgatgttg
    63121 ctactggcac atcgatcgtt ccaaggaatc cgtaacggaa accggagatc atataaacga
    63181 tcggattgac ttttgaaaca gcttgccaaa agggcggtaa catagagatg gcatagaaaa
    63241 cgccacctag ataagttaac ggctgcaaga caaatgtagg cacgatcgat acatcatcat
    63301 aagactgagc aaaaatcccg ttgagcagtc ctgctaatga aaacaagatt gccgtcatca
    63361 ataatgtaat gatgaccatg aaccatgaat acacatgaag tggtacaaag aacaaggaaa
    63421 tgatcgttac gagagagccg acgagaatac tgcgacccac accaccaatc acaaagcccc
    63481 aaataatcac atgcgtagga acaggtgcta ccaagatttc ttcgatattt ttctgaaatt
    63541 tttgagagaa gaaggacgaa gaaacattgg catacgaact agtgatagca gacatcataa
    63601 tcaaaccagg aacgatgaat tccatatatg aaaaaccacc catgtcaccg atccgcccac
    63661 cgatcatttt tccgaaaata acaaaataca aggatgtcgt gatcactggc ggaaccagtg
    63721 tttggaccca tatacgcaaa tagcggttcg tttccttgat agctaaactt tttaatgcag
    63781 taaaataaag attaaacatt tttctcctcc gtatgattat tttcttctgt gattttcaag
    63841 aacagttctt ctaaacgatt cgacttattc ctcatagaaa gaactttgat tccttgtctt
    63901 gtaagctgat cgaagattcc gttcacccct tgatttcgtt caacttcgac agcgattgtc
    63961 tgctcatctt caaatgtaca agcatatcct tcaatcacag gtttggtatc atatggcgct
    64021 aaatcaaaga taaatgtttc aaattgcagt tttgacagaa gctctttcat acttgtattc
    64081 tcgatcagtt cacccgattg gatgattccg atgttgcgac aaagcatttc agcttcttct
    64141 agataatgcg tcgtcaaaat gatcgttgta ccattttcgt tcaattctcg tagaaactcc
    64201 cacatctcgc gtctcaactc gatgtcaact cctgctgttg gttcatccaa gatcagcaaa
    64261 cgtggttcat gcatcaatgc cctagcaatc atcagtcttc gcttcattcc cccggaaagc
    64321 atacgtgcac ggacatttct cttttcccaa agattggact gcttcaaata tttttcgctg
    64381 cgtttcatcg cttctttgcg agagacaccg tagtagcctg cttgattcac gacgatttgc
    64441 tggaccgttt caaaagggtt aaaattgaac tcttgtggaa ccaatccaat ctgttgcttt
    64501 gctcgaacta gatccttgtc cagatcataa ccgaagacat tcacttttcc agatgtttta
    64561 ttgactaaag aagtaataat cccgatcgtt gtagacttcc cagctccatt tggccctaat
    64621 agcgcataga aatctccttc tttcaccgaa agatcgattc cccgcaatgc ttcgacccca
    64681 cttccataga tctttttcaa attatttatt tcaagcgcat ttgccattta tttcctatct
    64741 ccttatagac atgattctta attgtatcat ggaatacagc agatgaaaaa acaacttggc
    64801 ttccacacaa aaacacacct gctaacaaag aggagatgtg ttttctagtt catgatcatc
    64861 ttcgatagaa taactatcta aacaactctg gaacttttaa aatcataaaa gtaccgacag
    64921 caactactac gatgacatat agaagatcct tataactgag gttcttgtct tttttattca
    64981 tgttaagctt tcctccgtcc ttttatcgaa agcttttttc ttttatttta accgaaataa
    65041 ataaaatgga aatagttatt ttcaatttct gttttcgctg tttgttttac ggagaacttt
    65101 gccatggaac gtgcgtctcg tcgctgatgc taagtttttt cgataaaagc tgctagaagg
    65161 aagacagcgt ggtttatttt gtatcgtctt ttgctaaatc ttccaagatc atctggacag
    65221 cagactcgtt cccattgaat tctgccactt ttttggcatc aatgtacttc ttttgataat
    65281 cgtctttttg gacccacgca tcatgcttcg tttgataaag ttaatcaaac ggataccact
    65341 gacgctgttc atcgaacaac aaactttctt tttgggcctg ttcaactatg gatttaggat
    65401 aattcaaaac tttcagcaag gcaatcgcat ttcgggtcct tgaaggtcct tcttttaatt
    65461 tgaaatcaaa actgattcct tttccttctg ttacttgttc ttcaaagtgg acattctcac
    65521 aataattctt taaaatctct gtcaattcga tatcatgtgt ggcgacaaat gctaaagatg
    65581 gatactccgc aagccaccga acgactgaag cggaagaagc aatccgttcg atcgtatttg
    65641 ttccttttaa aatctcatct acgaagcagt agcagcgttc tttggaagcc acttggtcca
    65701 gcaaacgttt gatcgatttg atttctgaaa caaaataact gtcgccttca aataaatcat
    65761 cttcgattgc catggaagtc aacacatgtc caggttgcag ggtaaactgt tctgcaatcg
    65821 ctgtttggat cgtctgtgct agaatacagc tgatggctat acttttgaca tacgtagatt
    65881 ttcctgatgc attggaaccc gtgactaatg tattcttctg ccagttgact ggattaacta
    65941 ccgcttcttt taacaatgga tgatagatat ctttcgctgt cacaccgccc tcagtaaact
    66001 ctggtcggca agtgatcggc atataggtgc gataattcaa aacggctgct gcaacttcca
    66061 attctcccag caattcccac agcttgatgg cttcagttga atgcttttct aatttagtga
    66121 gaacgaaatg ataggagata aatggcagca tgaacatgat gttgacatat tcaaaaatca
    66181 attctgcttc actcccgctc ttcattcgaa atgaaaaacc taatttaggg atgtctctta
    66241 atggtttagt ttgctgtttc agctcttttt gaagtggtgt cgtcattttt gctaactgat
    66301 tggcggctga aatcgttgaa accaaataac tcatgctatt cagctctgtt tctagtgttt
    66361 gtttcttcac tagataatag accgtattga atactaagcc accgattgct agatagattc
    66421 ctagtacttg ccctaaaaga agcaaaccta cacccaccag cggaagcaag ccaagaaaaa
    66481 taaataaacc tacgcgtcca atctttttac tttttccatc cgctagatat tgttttgcaa
    66541 aattatgatc tttttttcct aaacgagcga attgatattg gattctttct ctcagttgag
    66601 gattttcctg ataaaaagca attagttttt ctaactgata atcttctcca aaatcaaagc
    66661 tacgtaaacg ctgatacaac gcttctgaac ctacactaga ataggtactg ttgatccctt
    66721 caaatacttc gaacatatcc aagtcatacc aagtaagatc gtcgatttca ctatcccatg
    66781 aacggaattt tttctccgtg agccacgcat ccttcaaact ttcttcttta tcaaatctcg
    66841 gttgataagg aatttttccc caattactac gaactgtttc ctttactttc aaacgtgaaa
    66901 aaatgtctaa aacaactaat aaaataacaa ctgcaataat aattaaaacg ataaattctc
    66961 tttggcccat atccctctcc gttccttgat ttgttagttt tagtatagca ctgttgcgct
    67021 atttgaaaag ggggtacaaa aataactgtc ttcgttacct aattcgtatc aatcacacca
    67081 aaataaaaac accttccaaa aaagaagatg tctttaaaac gagatacaat tttttgtcat
    67141 tcacttcagg taaattagag ccaattaaac cctccgcaac ttgacggaga tggagaagat
    67201 gcccccgtct aatctagcgg tcatctctcc tcccattcct tccaccaatt tttttgacag
    67261 gtaaagcccg attccgctag attccgtatt tgtgcggctg gtattttcgg tataaaaacg
    67321 ctggaagact ttttcaacag aggtttgcgc tcctggtttg ataggattgg ccactgttaa
    67381 tcgaatgaat gaatcctctt ttgaaaatgt cacctttgct tgtccatctg cataacgcaa
    67441 taaattctgt atgatatttt gcagcactcg cagcaacatg agcggatctg ctaatacagt
    67501 tgtatttatc gagtgttcag cgaacgctac ctcgatattt ttctcagaaa attgttcata
    67561 aaaagagaaa aatacttctt ccacctcttt agaaaaagaa agcgtatgga gatccatttc
    67621 tttttgcttc gtctctattc gagcaagatc ataaaactga tccgtcatac tgagtaatct
    67681 tgcaacagat tgtttgatga tcgttaaata cttctcccgt ttctccgtat cctctacctg
    67741 ttccagtaat tgtacataac cgttaatagc agtcaaagga gttctgagat catgagaaag
    67801 gccgacgatc gatagtctga tgttttcttc catttcttga aatgcttgtc ctttttgttc
    67861 attttcatcc acccatgtat tcagtgcatc tactaggtcc attagattct tttctcgaaa
    67921 ttcgactgaa agacgaccac catattcttg tcgttcagaa agagtggcga ttttcttagt
    67981 taatctcttg atttcccttt gcaggaagaa aataaaactt cccataaaga taacaacgag
    68041 aagcaacagc cacttaatca aatctaaacc ccactcccca caccgtatga atatacacag
    68101 catgtgcctt tgctagtttc cctcgaagat tggaaatatg catattcact gttttctcac
    68161 tatcaaaata tggttcttgc caaactgttt cataaagatt tgctcgcgaa aatacttttt
    68221 tcggatgttc taaaaaaagc ttcagcagat cgaattctct tcctgtcaaa tggacagcca
    68281 caccattcac atttacatcc ctcgactctt tgtccagtaa gatttccttg taatagatgc
    68341 gttgcgacag atcttttact tggacagaag tctgatgacg aagttggatg ccgattcgta
    68401 acaacagttc ttcgatatca aatggtttcg tcacatagtc atccgctcct gctgccaaca
    68461 aggaaacttt atctctttga tcgctttttg ctgaaagaac gatgatcgga acattcgatg
    68521 tctgtcggat ttctgctaac aatgcttcac ctgaaagccc tggaagcatg agatctagaa
    68581 ggatcaaatc gacaggatat gtgcttaaca ataattttcc ttccgtacct gaataagcat
    68641 caagaagttt gtatctctgt tcaagaacct cctccagtaa agaatgaaca gcttcatcgt
    68701 cttcgataat caaaatagtt tccagcagga acacctactt aatgtcagat ttttggaaaa
    68761 taaacattcc tagagctgat gataccacga ttgttactag actgaccaga atcattttcg
    68821 gtgcttgcga ataagttgct tgttgcgcaa agtccgtata catgagccac gtttccgtaa
    68881 accactccgt tagaaattcc aagcctttga tttttgcgaa aacaccggga acgaaggccg
    68941 ttcccatgat ataaagaaaa gaaagaagca aactgacact ggtacttcta gtgatgaatg
    69001 tcaaagtcac tggaacagat aatgtcgcta atatcagcag aagcaataca ggaaatactt
    69061 ttagaacata atttccatag tttttgattc cctcaacttc accaaatccg aaagcaagtg
    69121 tgtaagcaat catggctagc cccgtaatca aaaccacgcc aagtgttgtg atcaaaagcg
    69181 taacgagaag tttcgacaca taataagtca ttcgattccg tcctaaagat aacgtattgc
    69241 gaatcgtacc gttttgaaat tcttccccaa tataaaagca cgcaaatgct gcgaaagcca
    69301 gaagaatcca tgcagccatt ttttccacca ttaaaatccc tgttgcaccg ttgatgactg
    69361 tatccgttgc ttggtttaat tcattgccgt attttgtatg catataccat tcgttccaag
    69421 aataaaaaag cggaagtacg accatcagtc ctagtgagac ccacatttta cggctcttcc
    69481 atacttttat tttttcagca gttaatagat taaacatgtg gattgcctcc agttaattgt
    69541 aagaaataat cttccagttt ttgcccaact aggtggatac catccacagg aacttttgcc
    69601 tctaccaatg cttgattgat ggctgctact tggtcaattc cttcaaaaag ctggataaca
    69661 cggctagatt ggacaaaata atccctataa ccaagagagt cgagaaccgt tactgcttgt
    69721 tccgtcgccg aagtgtcaat ttttataaat tgacgagatt cttgtgccaa ttcttctttc
    69781 gataattctt tgatcaagtg tccttcatgc aaaataccgt agtgagtggc gatctgtgac
    69841 aattcatcta gcaagtggct agaaagcaag acggtgatgc cccgttcttt taccaaacgt
    69901 tggatgattt ctcgcatttc tgccatccct gctggatcca tcccattcgt cggttcatcc
    69961 aaaatcaaaa attctggatt cgtgatcagt gcatttgcaa tagctaaacg ctggcgcata
    70021 cctaaagaga aattctttgc tttctttttc cctgtatttt ccaatcgcat cattttcagc
    70081 aggtcttcga tttctctatc cgacacaccg ccgttcgctg cttggatacg taggttatct
    70141 cttgcagtta attctggata aagagcaggg gtttcaatcg attgacccat acgtcgtctt
    70201 gctgcctgca aatccgtcgt tccaaataat tcgatcgaac cttcatcaat cgagatcaaa
    70261 cccataatag ttctcataaa ggtagactta cccgctccat tctccccaat caaaccatag
    70321 atcattccac gtttgatttc gatcgatact ttatctaatg ctttagcagc accgtatttt
    70381 ttggttatac cagttgcttt caagaccgtt tcatttccaa aatccatact tatccttctt
    70441 tcttattgat aagtgaagta tagttgacgg gtataaagaa agcagccaga aatgtataaa
    70501 gaaagtgtaa attttccaat tgaatttcaa gaaaaatcag actgtctcgc agacgtggtt
    70561 gagttgtctg ccaatcaaag agcgcaccaa acagtgctaa tccaagtgga ctggccacca
    70621 tggtaatcgt tgttatcgtt gacatcagac gtccttgtat atgcgttgga acttctcgct
    70681 gaagccagac catataaaaa gcaaaagaaa acattaatcc ttaaaaacgg acttatgaaa
    70741 aataatacat gccgtttggg atttacaatt agctaccaaa taagaggtta tgacaaaaga
    70801 cttttcacaa tcccttcttc cgaataaacg gtgttctcct gccaactcct cggtcacaag
    70861 tcacaatatt tatcaaacat gagaagcaat ttttgtaaat tccttcttat ttcttgaagc
    70921 tgaccacttc tgtcacaacc tcagaagcgg ataaataata ttgtttgatg atactttatc
    70981 tttttcccgt ttgtttatag atcgaatgat attatagacc aagaatccta cctattaaca
    71041 tagctaaaaa cacaaaaaag gcactttcca aattgtggaa agtgccagaa aagttaggat
    71101 taaggtaact gcgctcagca ttgcttcgcc cagtacgatt ctcgtaactc gtgaactcga
    71161 acgataatcg caattaacgt tttgagaact gtgaagcttt acgggctttc ttaagacctg
    71221 gttaaacaaa actctatttt attgcatttt acaggaatgt atttcatgcg aaattaataa
    71281 gtctttgttt atagagtttt actagggtat aaaaagttgt atgttatcat tacctcaaaa
    71341 cattacctca gcttggcgag ttggttgatt gaagaataaa tcatttatct agatatctca
    71401 ataaaaaatg atttctaaga aacttgtatt ttcgtgttat tatttttttg ccctatcaaa
    71461 atttctaatt cttctagcgt aatatgatta tgacacataa tcgattcaat tacttcaata
    71521 cctatttttt tcgagaaaga tcccttttgt tcactcacaa aaatactttc agaagacata
    71581 tataaggaac gaagttttgg aattagtata tcgtacaaat agaccaccat aaaatcgtcc
    71641 ttgcaaccat tctcaaaaag aattaaattt gctgattgtg ttacatatat cactgaatta
    71701 cattcatggt caattccaaa aacaatgtct acttcatttt cttttctaat tcgcgcagtt
    71761 acatttttca aactttcacc attacttata aatgctttag ttactgtaag ctctgatgtt
    71821 tgttcagata ttccttttat aatattttgt tcatcagcca cattccccat aaatccgccg
    71881 attcctttta agtaataatt ctcacctaag agtcgcttgc attcactgaa ttgataaaaa
    71941 aaccagttaa aaaaatcttc tggaaaaata aattctgatc gaactttatt tatattttta
    72001 gctcctatta attttataac tcgattaatg ttcacttgct gcgatgaaca tataactgaa
    72061 taaattgagt tgtttaattc aaaaaaacaa ctttaacata tgcatcatta attcgttatt
    72121 ttttaggtag atattgatca ttgttatctc tctttttttg cttcccatac tccaacttag
    72181 aaattgccgt tataaaattt attttaaagg tgactttttc ccctgaagta tcggtagtag
    72241 gaatacttaa tacctcagct tctgaggagg tatcccaatt atagttatac tttgcatcat
    72301 caccataaag aacaattcca ttttttttat cttgtgccgt tagctttact ggttcgctta
    72361 cttttcttgt taatatcttg gactttacat catcgatatt gcttatttta tcaagcttaa
    72421 ataatttaca aattaatttc gtactagttt ttatatttga cgagagtgta aaatattttg
    72481 tgtaaataga aaaaaggaag tcccttctgt agaatagagt taccacaaca cattcacaga
    72541 aaagaggact tccatat
//
LOCUS       DACGLE010000006        64947 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0534
            SAMEA3304449-rid9560453.denovo.006, whole genome shotgun sequence.
ACCESSION   DACGLE010000006 DACGLE010000000
VERSION     DACGLE010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304449
            Sequence Read Archive: ERR985064
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 64947)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 64947)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702225.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702225.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 20:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,045
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 27 of 67
            Pseudo Genes (incomplete)         :: 25 of 67
            Pseudo Genes (internal stop)      :: 19 of 67
            Pseudo Genes (multiple problems)  :: 4 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..64947
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304449-rid9560453.denovo.006"
                     /strain="VREN0534"
                     /db_xref="taxon:1352"
     gene            299..1960
                     /locus_tag="IXT20_000490"
     CDS             299..1960
                     /locus_tag="IXT20_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HAQ4858966.1"
                     /translation="MKKKFTFGLVAVCGLVLAGCYGGSSATSSSSAKSGSADGGGVFN
                     LVVPQEMPTADLSVATDTISFTALNNVYEGIYRLDEDSKPQPAGASELAEVSEDGLTY
                     KIKLREDAKWSNGDPVTAADYVYGWQRTVDPATASEYAYLYAPVENAEDITAGKKDKS
                     ELGIKAVGDYELEIKLTKQTPYFQYLLAFPSFFPQKQSVVEENGDAYASASDKAVYNG
                     PFTLEDFDGPGTDTEWTYKKNDHYWDKDTVKLSEIKVSVVKESSTALNLFKDGQADDV
                     ILSGELAQQNANDPAYTSVKEARTSYIEFNQREKNSPFNNVNLRKAISYSIDREALVK
                     QVLGDGSVVSTGLIPSDMVKSPTTNEDFAKEAGKLVSYDKDKAKEYWEKAKKELGVDS
                     LEFDLMASDDDSSKKVIEYVQNSIQENLDGVTVKPTPVPFSVRLDRSTSGDFDAVLGG
                     WAADYADPSSFTDLFVTGNSYNRGRWSNAEYDEAVKAASSTDAGDEQARWDDFQKAIK
                     IISDDMGVVPVYQKAEGHLVNEKIKGIVHHAAGASWDYKWTYVEE"
     gene            complement(2030..2377)
                     /locus_tag="IXT20_000491"
     CDS             complement(2030..2377)
                     /locus_tag="IXT20_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3899 domain-containing protein"
                     /protein_id="HAQ4858967.1"
                     /translation="MKKKSIPYAVAFLLILVILIKNLINHSFTLIQLSNDLFLWSLPF
                     LIIGGFLWVFSSGFFDHFQRSVHLARTRNRKKKPEFSSLSSASYGMYSFWLIIAGILI
                     ALSAIFMLFSLLG"
     gene            2461..3402
                     /locus_tag="IXT20_000492"
     CDS             2461..3402
                     /locus_tag="IXT20_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ4858968.1"
                     /translation="MNSYIKYVLKRVFFMIITLWLIATITFFLMQLLPGTPYTNQERL
                     SPETIEMLNKQVGLDKPVIVQYGIYLSNLLQGDFGISFQFKNQPVAHLLAGRIGPSLQ
                     LGLQAIIFGTVLGTILGTISAMKQNTWADTSSTLVAILGRSIPNFVFAVLLQYIFAIK
                     LQVLPIAKWDGFVYTILPTIALAMSPLADSARFIRTEMVEVLHSDYVELAKAKGLSRW
                     EIAFKHGLRNSLIPLMTLLGPLAVALMTGSLVVENIFAIPGIGEQFVKSITTNDYPTI
                     MAVTILYSFMLIFVILIVDLLYGLVDPRIRVSEGSRG"
     gene            3403..4446
                     /locus_tag="IXT20_000493"
     CDS             3403..4446
                     /locus_tag="IXT20_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ4858969.1"
                     /translation="MEMIPKRYETVADIPADEFLPLQKNTEEEREQITAPSLNFIQDS
                     WRRLKKNKAAVISMVLLLVIILISVITIFVSPHDPTAQNVAYINLPPRIPGINIDGLN
                     GKAMVGGELVDKYAQANVPSNVNFFLGTDGLGRDVLSRLFMGMRISLLIAFIAALLDV
                     TIGVAYGLVSGLLGGRVDNVMQRFLEILSGIPNLVVMILMLVVFEPGIFSIVAAMAIT
                     NWIPMARIVRAQTMKLKDQEYVLAGLTLGESRMKIAFKHILPNISSVIIIQMMFSIPS
                     AIFFEAFLSFIGLGLTPPSASLGTMLSEGYKTFLYLPYLLWIPAVTLSVIMIGFNLLA
                     DGLRDAFDPKMKE"
     gene            4452..5522
                     /locus_tag="IXT20_000494"
     CDS             4452..5522
                     /locus_tag="IXT20_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ4858970.1"
                     /translation="MTKILEVKDLEISFDTYAGKVRAIRGVNFHLNKGETLAIVGESG
                     SGKSVTTRSIMRLLSSNANIDAGQILFKGNDIVGKSEREMQKIRGKEIAMIFQDPMTS
                     LDPTMPIGKQVAESLRKHNKVSKKEGQKAALELLKLVGIPEAEKRINSYPHQFSGGQR
                     QRIVIAIALVCYPEILIADEPTTALDVTIQAQILELLKEIQTKIDTSIIFITHDLGVV
                     ANVADRVAVMYGGRIVEVGTSEEIFYNPQHPYTWGLLGSMPTLDSANDRLYAIPGSPP
                     DLLNPPKGDAFYPRNEFAMKIDAEQEPPFFELSKTHKAATWLLAPQAPKVTPPEEIQR
                     RWAIFKEKQKHTYGQGGIADAK"
     gene            5512..6465
                     /locus_tag="IXT20_000495"
     CDS             5512..6465
                     /locus_tag="IXT20_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ4858971.1"
                     /translation="MPNKLLEVKGLKQYFNVGKKNEVHAVNDISFHIYEGETFGLVGE
                     SGSGKSTTGRTIIRLNEPTDGEILFDGQDITKIKDKKGLTKFRHDVQMIFQDPYASLN
                     PRMKVRDIIAEGIDVNGLAKSSEERAKKVDDLLRTVGLNPSHGTRYPHEFSGGQRQRI
                     GIARALAVKPRFIICDEPISALDVSIQAQVVNLLQDLQKEHQLTYLFIAHDLSMVKHI
                     SDRIGVMHNGLLLEMGTSDEIYNHGVHPYTESLLSAIPLPDPDHERKRRRIKYQPEPD
                     DGQVRQLREIAPEHFVYATEQEVPYYAKKLKRQKESLLVAN"
     gene            6477..7487
                     /locus_tag="IXT20_000496"
     CDS             6477..7487
                     /locus_tag="IXT20_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAQ4858972.1"
                     /translation="MLKAFKFRMYPTEEQKQQLIRTFGCARFTYNHLLKKRQKSWQQT
                     GVANFSLTPATLKKEYPFLKEVDSLALANAQLNLDRAFRNYFKGRASFPKLKTKKSMW
                     QSYTTNNQTRTIYLENGYLKLPKQKELIKINQHRPVEGSIRSATISARYNEEFYVALL
                     CDVSPVKKESLAKWIGIAYHPKTLIQTSRPLEVTLPKFHQTEEKLQHAQRKLNVKVRS
                     AHHRKIRLDQASNYQKQKRKVMDLYLKQKNQREDYLEQLSGKLVKQYDYLFVESFPKE
                     EAHADFSIHDWHKLITKLRYKSQWYNKKFLLINTDGAEESNSVRKSQVVEKMGRHSVI
                     KG"
     gene            complement(7772..8593)
                     /locus_tag="IXT20_000497"
     CDS             complement(7772..8593)
                     /locus_tag="IXT20_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HAQ4858973.1"
                     /translation="MNRFASKEEEKLYYEHKASEEEFLNWYQKQERPEYDKPSLTVDI
                     VLMCYNKEEDQLKVLLIQRKGHPYRNSWALPGGFVQKDESTGESVLRETKEETGVVIS
                     KENIEQLHTFSTPNRDPRGWVVTVSYLAFIGEEPLIAGDDAKEVRWFTLERHSNKIHL
                     SSGEVAISLDLVTGESSGKDTLAFDHSQIILKAFNRVVNKMEHEPQVLQVLGKDFTIT
                     EARKVFAKFLGIDYKTIDHSNFKKAMLQYFDEIGERPVGIGRPSKIYQLKPGHHE"
     gene            complement(8690..8968)
                     /locus_tag="IXT20_000498"
     CDS             complement(8690..8968)
                     /locus_tag="IXT20_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4858974.1"
                     /translation="MQKKHLFFTLSIAFLSLAHLIFSYFYIRMYGYFNLHGHLNSFMT
                     AAWILRFIIDVYIVICGFFAIREERYKVLPFYLLFFLFNLILPFIFHI"
     gene            9236..11245
                     /gene="metG"
                     /locus_tag="IXT20_000499"
     CDS             9236..11245
                     /gene="metG"
                     /locus_tag="IXT20_000499"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HAQ4858975.1"
                     /translation="MAEKETFYITTPIYYPSGKLHIGNSYTTIACDAIARYKRLMGFD
                     VFYLTGVDEHGQKIETKAEELGVKPQEYVDKMAADVKKLWKTLDISYDKFIRTTDDYH
                     KAAVQKIFDRLLEQGDIYLGEYEGWYSVSDEEFFTETQLAEVYKDDEGNVIGGKAPSG
                     HEVELVKEESYFFRMSKYADRLLEYYEEHPEFIQPESRKNEMINNFIKPGLEDLAVSR
                     TTFTWGVPVKSDPKHVVYVWIDALSNYITALGYGSEDESLFEKYWPADVQMVGKEIVR
                     FHTIYWPIMLMALDLPLPKKVFGHGWLLMKDGKMSKSKGNVVYPEMLVERYGLDALRY
                     YLLRAVPFGSDGVFTPEDFVSRVNYDLANDLGNLLNRTIAMINKYCDGLVPDYASLVT
                     PFDSELSTTAANVVGRYHDAMEKMEFNTALAEIWVLISRANKYIDETEPWVLAKDEEK
                     KAELNSVMIHLAESLRIAAILLQPIMTETPEKIFNQLGLDSETMNLEGLHFGEFPSGT
                     KVVAKGTPIFPRLDMEEEVAFIQEKMSEGTQTNEDTVKWDPEETELVSTKEKQIKFDV
                     FEKVELKVAEVINCQKVEGADKLLQFRLDAGDSQDRQILSGIAEFYPDPSELIGKKVV
                     IVANLKPRKMRGQISQGMILSAEAPDGSLQVIEAPKSMPNGSEIA"
     gene            complement(11724..12311)
                     /locus_tag="IXT20_000500"
     CDS             complement(11724..12311)
                     /locus_tag="IXT20_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4858976.1"
                     /translation="MDFKRSYPKLADYLSNRVDFSRKEQQQSHWHWFTLILFGLMTVA
                     TLSQKLILLTLFIAIVALIKGPVMILWGMLYSFLVGLFPPIGILLSAIFFLLNIGTFT
                     KNWRMTLVGIYFYFYPFGVMALSEVMHWDNHWFIAGSLLLGLILLHVMLTKLYQHYGI
                     GRTIFWYVFSIPFALLTALLPSRLKTKIKGYHKIK"
     gene            12486..13808
                     /locus_tag="IXT20_000501"
     CDS             12486..13808
                     /locus_tag="IXT20_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ4858977.1"
                     /translation="MKGKLKREINLFGALATVMGTVIGAGVFFKTAAVTASTQSTSLT
                     LLAWLLGGFLTICAGLTVAELATAIPETGGAVKYIEAAYGKLPSFLLGWAQSLIYFPA
                     NIAALSIIFATQLTNLLQLSTDYLLLIAVITAVSVTGLNLLGTKVGASVQSVTLIVKL
                     IPIAVIVIWGLLTPGQGTVQLFPIEAGKDVTFVEGLSSALLATLFAYDGWLGVGAMAG
                     EMKRPEKDLPKAIILGLSFVTVVYLLINFVFLKTLPIDHLAGNLNAASEASDVIFGGI
                     GGKLVTIGILISVYGALNGYTLTGIRVPYAMALEDELPFSKQLTKLSKKFTVPYVPAV
                     FQLAVACIMMSLGSFDFLTDMLIFVMWLFSLLIFIGVFILRKKAPELPRPYKVPLYPI
                     VPIIAILGAIFILGMTMMTQTKLALIGIGVTLIGIPVYYQKKKKRSEE"
     gene            13963..14739
                     /locus_tag="IXT20_000502"
     CDS             13963..14739
                     /locus_tag="IXT20_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HAQ4858978.1"
                     /translation="MIFDSHTHLNAEQFNEDIPETIQRAQEMGVTKMAVVGFDTPTIE
                     KSLQLSHDYPNIYSIIGWHPTEAGSYTKEIENKLQEQLTLPKVVALGEIGLDYHWMED
                     PKEVQDKVFRRQIAIAKEMNLPISIHTREALEDTYQILKEEDVRDIGGIMHSFSGDYE
                     WAKRFLDLGMHISFSGVVTFKKALDVQEAAANVPIERLLVETDAPYLAPVPFRGKRNE
                     PGYTRYTVEKIAELRNLPTEEVAAQTWTNAHRLFGLSEHG"
     gene            14732..15301
                     /gene="rnmV"
                     /locus_tag="IXT20_000503"
     CDS             14732..15301
                     /gene="rnmV"
                     /locus_tag="IXT20_000503"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HAQ4858979.1"
                     /translation="MDKMKIQEIIVVEGKDDTRRLREVVDADTIETIGSAINEEILMQ
                     IEHAQETRGVIVFTDPDFSGEKIRKIIMEAVPQAKHAFLPRTQAKPKKKGSLGVEHAS
                     DAAILEALKKVVTPDISQTAVSEITRQDLLDYGLIAGAQAKEKREKLGDELRIGYTNG
                     KQLEKRLKMFRITPKELAQAMKIVEEQHE"
     gene            15294..16178
                     /gene="rsmA"
                     /locus_tag="IXT20_000504"
     CDS             15294..16178
                     /gene="rsmA"
                     /locus_tag="IXT20_000504"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HAQ4858980.1"
                     /translation="MSEYREIATPSRTKEILLKHGFSFKKSLGQNFLTEPNILRKIVE
                     TAQIDEHTNVIEVGPGIGALTEQLAKHAKQVVAFEIDDRLIPVLADTMQPYDNVTIVH
                     QDILKTDLSTAVRETFHEELPLKVVANLPYYITTPIMMHFLESDLVVDELVVMMQKEV
                     ADRISAEPGTKAYGSLSIAVQYYMEASLAFIVPKTVFVPQPNVDSAILKLTRRDIPAV
                     EVTDEKAFFRLTKAAFQQRRKTLWNNLQHSYGKDDQTKAWLAKSLETAGIDPKRRGET
                     LSLQEFAALSNAMSENMQ"
     gene            16629..17258
                     /locus_tag="IXT20_000505"
     CDS             16629..17258
                     /locus_tag="IXT20_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4858981.1"
                     /translation="MRKNSVRKLTISALLIGMGVIIPMVMPKIMIPPASFTLASHVPL
                     FIAMFFTPGVAVAVALGTTFGFFLTTPVIIALRALSHLVFALIGAWYLQKHPSIVLKN
                     GQFTFANWRFQGFNFVIGVIHSLAEMLVVSIFYFIGNMPETYYSQGYFYTVFILMGIG
                     GLIHSLVDYNIAYFIAGTLSKYFDIPVFTAAKKENRKESIQGYAKKTTY"
     gene            complement(17381..18490)
                     /gene="ugpC"
                     /locus_tag="IXT20_000506"
     CDS             complement(17381..18490)
                     /gene="ugpC"
                     /locus_tag="IXT20_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HAQ4858982.1"
                     /translation="MVEMALKNVYKKYDNAENYSVTDFNLSIKDREFIVFVGPSGCGK
                     STTLRMIAGLEDISEGELNIGGKVMNDVAPKDRDIAMVFQNYALYPHMTVFDNMAFGL
                     KLRKYDKADIKKRVDNAAEILGLTEYLKRKPAALSGGQRQRVALGRAIVRDAKVFLMD
                     EPLSNLDAKLRVAMRAEIAKLHQRLETTTIYVTHDQTEAMTMADRIVIMKDGFIQQIG
                     TPKEVYDTPVNMFVAGFIGSPAMNFFNVTLKNGVITDGHGLKLRIPEGKNKVLVEKGY
                     EGKEVIFGIRPEDIHSEQVALEASPEAVVKSEVVVSELLGAETMLYTKTGSTEFISKV
                     DARDFHKPGEIVDLAFNISKGHFFDKETEQVIKLP"
     gene            18699..19121
                     /gene="mgsA"
                     /locus_tag="IXT20_000507"
     CDS             18699..19121
                     /gene="mgsA"
                     /locus_tag="IXT20_000507"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010763995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HAQ4858983.1"
                     /translation="MKIALIAHDRKKPMMVKLATAYKTILEKHELFATGTTGQRITEA
                     TGLPIHRFKSGPLGGDQQIGAMISEDQLDLVIFLRDPLAAQPHEPDVTALIRLSDVYE
                     IPLATNIGTAEVLLRGLEAGFLDFRRIVHEIDKHPLSF"
     gene            19444..19980
                     /locus_tag="IXT20_000508"
     CDS             19444..19980
                     /locus_tag="IXT20_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HAQ4858984.1"
                     /translation="MKKHRLDARMIAIMGLLIALMVTLSRLVAIETPFIKISVTFIPQ
                     VIMGILFGPFWSGIGAVLADLVGMALFSKSAFFIGFTLNAFIEGAIYGFFFYRKEITW
                     KNAILATLSVTLIINLFLTPLWLALMYHVPLFSWVVWAPRLLKTVIWLPIQSIAIYYV
                     GRSIPYKKILRSLAIHAK"
     gene            complement(20020..21783)
                     /locus_tag="IXT20_000509"
     CDS             complement(20020..21783)
                     /locus_tag="IXT20_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ4858985.1"
                     /translation="MKVKKLSLQTLRRFIPYLTAYAKDIWLAVILGIINGIATVVMTL
                     QIGQSIDQMIGEGQVRFNRLFHLLILFGGIVLINVVSQWLIQVLSNRLAYLSVAKLRK
                     DAFAHLNRLPLRYYDQNSHGNIVSRFTNDMDNISIAISAAFNQLFSGVAIIILSFIFM
                     MRLSPLLTLVVLCSTPIIFLVSWIVARASQTNFDRQQRIIGDISGFVTERIGNQKIVK
                     AFQQEEASQQQFELLNADLYEKGQRAQFSSSLTNPSSRFVDHLAYLSIGLVGGLLALK
                     SGSSITVGVISSFTIYSSQFSKPFIELSGITTQLQTAFSGLERAFDLIDQPEEKPDLP
                     DAYALEPKNIKGKVSFDHVSFSYDPKQRLIEDFNFTAYPGQTIAIVGKTGAGKSTLVN
                     LLMRFYDTTKGTITVDDHDIRSIQRDTLRKSFGMVLQDTWLFDSTLRENLRFGNPEAS
                     DAEINDALKASYMYEFVERLPEKLDTKIGGQGLKISDGQRQLLTIARTMISNPPMLIL
                     DEATSSVDTLTEKKIQQAFLAMMEGKTSFVIAHRLSTIKNADQILVMDQGAIVEMGTH
                     DELLQKDGYYRRLYQAQFTAQ"
     gene            complement(21786..23501)
                     /locus_tag="IXT20_000510"
     CDS             complement(21786..23501)
                     /locus_tag="IXT20_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ4858986.1"
                     /translation="MIQLLRYAKDYRKQIILGPFFKFLEAVFELVLPLMMASLIDNGL
                     KMNDRGKIIEMGLWMVAMSVIGLICAIICQYYASVASQGFGTELRNQLIKKINTFSHN
                     ELNHFGTDTLITRMTNDINQLQLALAMVIRLLIRAPFLSIGSVVMAFVIDWEVGLFFL
                     ALLPIFSIILFFIIKKTVPLYQKVQEKLDQLNEHVSQNLSGVRVIRAFAQTKKETEKF
                     DKSTNDLANNYLRVSNISALLSPATTLIMNVGIICLLTVGGIKVNIGSLQQGQVLALI
                     NYMNQMLLALIVVSNLVVIFTRAEASGNRVKEVLDTENSILEAEVSTTPDFSSEAIIA
                     FDHVDFRYTPDSGLSLQNITFQLKRNTVLGIIGPTGSGKTTLTQLIPRFYDVSSGTVI
                     MDGMDVRSWQLDHLRQQIALVPQTSVLFTGTIRENLQWGKKDATEKECWEALKIAQAE
                     EFVRQLPNGLDTKVMENGKNFSGGQKQRLTIARALIAKPALLILDDSLSALDYQTDLD
                     LRKALQTYLGSTVIIISQRVRSIQEAHHILVMDQGKIAAQGTHEELLAQSAEYQEIVA
                     SQEEE"
     gene            complement(24280..24504)
                     /locus_tag="IXT20_000511"
     CDS             complement(24280..24504)
                     /locus_tag="IXT20_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4858987.1"
                     /translation="MKSDKYKNRKHTSWKTFREIIGSYLAVVVFLPLLYMAMTEQTRF
                     IGVDTQYIAETGFLFIFILIFAFYKSFHTK"
     gene            24704..27472
                     /locus_tag="IXT20_000512"
     CDS             24704..27472
                     /locus_tag="IXT20_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ4858988.1"
                     /translation="MLKKEILCYLENQTAFFDLEHMNEVFTAQRLADHFGVKRNTVSH
                     YLNQLNDEQLLVKINSRPVIYFHKEAFEKQFFKLRTNFYFSIDDLKEEQPFFAQPKDL
                     FSFMIGYDASLKESIEQIKTALYYPDGGLPLLITGESGTGKSYLVNLVYQYCLLHDLL
                     EDSAPFITFNCAQYADNPELLTSNLFGHVKGAYTGAEENKKGAFEAADGGILFLDEVH
                     RLTPEGQEKLFTYLDQGVIYRMGEPNLSRRIKTRLFFATTEEITSHFLTTFIRRIPIR
                     IELPALNQRSRNERLELIYSFFLEEQRKMGSPLSVSGPALSLLTSQKLSGNIGELKNI
                     VKVSAAKAYAEQREQPEIHVTIHHLQKELLAQPITKNTAQQGIYITQDQTLEQLMEKQ
                     QPEQQRIVKSFEQILIDFKKNNCLLYSCEGKLKQEVIQLFDFLLFETSRQEKHELLVY
                     WTQSIRETFRQMETAYQIKFDGNSVYALSYYLYQRSTIKWLPEEHDIIQLIKELEQQV
                     SQSYPSSYHYVQRILELSKIKLDIEDTSMDRILLTIYLKKAKWSKEKRLPKAMIAAHG
                     YATASSMANVVNRLLQEDLFESFDMPLDVTPQQIASEILDYCENSDVSNGLIILVDMG
                     SLKEIHQFFKKQLSVPLLILNNVTTPLAITVGECLQKNVSLEEIAEETVRQIQPEWRL
                     LYPEENKPKALITTCFTGIGTAVHLSELLEKSLPTACQLKIIPYEYQQLKDKKNSDPL
                     FSIYEVVGMIGTTDPDITNIDYLSLEDLISGEKMSVLAEWLDTTMNASEKEIFNQRII
                     RNFSLEKVLDSVTILDTEKVMGEIDHFMRELEVQLNRSISNPKKLALYVHVSCLIERL
                     IRQMPIETYQGLEKWKQCQKEGLSAIKSAFSVIEEHYSVMIPNSEIAYIYDILSEDTE
                     FLSIEEEF"
     gene            27553..27972
                     /locus_tag="IXT20_000513"
     CDS             27553..27972
                     /locus_tag="IXT20_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIA"
                     /protein_id="HAQ4858989.1"
                     /translation="MDREIILASHGKFASGILDSLELIYGKNHSITVFDCYTDENFDL
                     AETVRQLFMRYKDKEIIVLTDLFGGSVNNEFLQYINRDHVYLVAGLNLPLLIELVSRL
                     DAEVETTVLIQQVLTDSKKMIQFCNERLNQKIEEDEF"
     gene            27984..28454
                     /locus_tag="IXT20_000514"
     CDS             27984..28454
                     /locus_tag="IXT20_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ4858990.1"
                     /translation="MIVLTRVDHRLLHGQVAFSWTQTIGADCILIANDDVPTNEIRKT
                     TIKLAKPQGVKLVIKSIDDSIAALKSGVTDKYKLFIVVESIEDAYKLAEAYPEIKTIN
                     LGGIKAKPGTRSIGKAVNILPEEEERLKRLIDKQIEVEIRQVPADKKVDVREVL"
     gene            28471..29250
                     /locus_tag="IXT20_000515"
     CDS             28471..29250
                     /locus_tag="IXT20_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ4858991.1"
                     /translation="MIVQAVLLGIVAFVAQSEYALGTSLLSRPIVTGLFTGIVLGDVK
                     TGIIMGATLELAFIGSFSVGASIPPDVVTGGILGTAFAITAGAGTETALLLGLPIATL
                     TLILKNIYLGLLIPIMNHKADNYAEEGNYKGVEHMHLLAGIGLSLMLGVVVMVSYMVG
                     SNTIGNLLNMIPDFVQKGLSVATGLIPALGFAMLARLLINKQVAPYFFLGFAIASYLE
                     IPVTGVAIFGAILAVVVVNIMNVRQLPLQTTAGEVEEDEEF"
     gene            29237..30058
                     /locus_tag="IXT20_000516"
     CDS             29237..30058
                     /locus_tag="IXT20_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAQ4858992.1"
                     /translation="MKNSEIKNSKITKKELNHVFWRSFQMEFSWNYERQMNMAYVYAM
                     IPILKKLYQSKEEMAAALKRHLEFFNTTPHIVTLILGITAAMEEENKDDPEFDVTAID
                     SIKTSLMGPLAGLGDSFFWGTLRLIATGVGTSLAMQGNILGPILFILIFNIPHILFRY
                     LATGWGYRLGTGFLKKIQANGMMESLTLGASIIGLMVVGAMTATMITINIPLKIGSGE
                     NAVTVQSIFDDIVPNILCLGAFGIVFYLLKKEVKPLTILIGLAFFGIFGSWIGIF"
     gene            30075..31124
                     /locus_tag="IXT20_000517"
     CDS             30075..31124
                     /locus_tag="IXT20_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAQ4858993.1"
                     /translation="MFTMQDYIYEEKEVLSTILKKNDFSTREHMKKTVNLLILATGSS
                     YNACLAAKPALESYGDLTVDIQEPFYFFNYGKLSPSIDTVIAVSQSGKSASTVEAVKM
                     IQKQGLPVVAITNDVQSPLALEAAQIIDLGAGVESVGFVTKGYSATVLQLLLLGIGIG
                     ISKNKISKKIEQDYMQQLRKIINHLPAIIQKTEEFFDGYQSLFRLAQRFVTIGYGPNW
                     GTAKEAETKLTETIRVPSQGFELEAYMHGPYLEADASHLLFFIEGESVNKERSQKLQR
                     YMSRYVGETLTITTKKARNEKTLGLAIECDEYLSVLALVVPFQLFAYKTAVAKGIDLN
                     KKIFEDFDTVLKSKL"
     gene            31137..32141
                     /locus_tag="IXT20_000518"
     CDS             31137..32141
                     /locus_tag="IXT20_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAQ4858994.1"
                     /translation="MLKFNEEEQIKAIKGALALRPQVEEIIDKLYAEKFDAVYYLGIG
                     GTYASSMQAVTYMNGKSNLPVFVQHAAEYYTTGNKRLTKDSIVILSSVTGTTQEVVKA
                     VEQIKKVGATLIGFIDKANSKLSQLCDFVVTYPAPGTEQIKFFMAADRLMYLNGEFEA
                     YSEYYEQLERYLPTGLVEAEKKADAFGLSFAEKHRRDSMHYFIGAGNQWGAVYSYAMC
                     YWEEQSWLPSKSIHAAEFLHGTLEIVEETTPVTLFLGEDEQRVLSERVAKLLPKICSN
                     YTLIDTKDYPVEGISEKYRGRVLSFLLMHVVTQRIDAHVEQLNCHPLEIRRYYRQFDY
                     "
     gene            32774..33793
                     /locus_tag="IXT20_000519"
     CDS             32774..33793
                     /locus_tag="IXT20_000519"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HAQ4858995.1"
                     /translation="MKQKIAVLGPGSWGTALAQTLAENGHDVRIWGNVPEQIDEINKY
                     HTNQHFLPDLTIPESIIGYKELAEAVDNADAILFVVPTKAIRSVAQELIGKMNTKPII
                     IHASKGLEQDTHKRISEVLAEEIPEEKRQAIVVLSGPSHAEEVAVHDITTITAASIDQ
                     KAAAYVQKLFMNDYFRIYTNNDVIGVETGAALKNIIAIGAGAIHGLGFGDDAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGVGDLIVTCTSVHSRNWRAGNLLGQGHKLEEVLE
                     NMGMVVEGVATTKAAVELAQQLGVEMPITQTIYNVLYNGEDIKKAAKEIMLRDGKMEN
                     EFSLR"
     gene            33836..34717
                     /gene="galU"
                     /locus_tag="IXT20_000520"
     CDS             33836..34717
                     /gene="galU"
                     /locus_tag="IXT20_000520"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HAQ4858996.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LDSGIEDILIVTGKEKRPIEDHFDANIELENNLREKEKTELLALVEETTQVNLHFIRQ
                     SHPRGLGDAVLQAKAFIGNEPFVVMLGDDLMKDDIPLTQQLMMDYEETQASAVAVMRV
                     PENETSKYGIIDPKTDSEKGRCRVKGFVEKPEMGKAPSNLAIIGRYLLTPKIFEILET
                     QEPGAGNEIQLTDALQTLNQTEAVYAREFKGKRYDVGDKLGYMKTNIEYGLQHSEIGT
                     SLSAYIIQLSKKLRTES"
     gene            35017..35394
                     /locus_tag="IXT20_000521"
     CDS             35017..35394
                     /locus_tag="IXT20_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4858997.1"
                     /translation="MGKLVAVAQSLLDILKWLMEGSNWIFVLPLLLISLFIMFRMVRK
                     KETFLIFIWYAASVGVATLMVELALGSWEACVFALFFAVLYLIIYIPYMKRKSRIGRK
                     GRHTTTFSSTDSLEQSRRTRRRR"
     gene            36000..36614
                     /locus_tag="IXT20_000522"
     CDS             36000..36614
                     /locus_tag="IXT20_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAQ4858998.1"
                     /translation="MSLCYFDYYHQLEEKLRIEEPLMLQAGTWIAKQINQGGRLQIYA
                     SRTLSGVAFEFWDQTPDLVPSILIENPAGGVYETLEGAGQAIIDQLSVKSEDIFLLLS
                     NEGRNPAIIELAQWIKENGHPLIIVTGFDLSRSIKSQHSSGLRLFEFADLVLDNHANL
                     HDAVLTLPNLDPAICGTASLATIFLLQQVLYFTAKQLVQDTSGK"
     gene            37387..39126
                     /gene="yfcC"
                     /locus_tag="IXT20_000523"
     CDS             37387..39126
                     /gene="yfcC"
                     /locus_tag="IXT20_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HAQ4858999.1"
                     /translation="MKKTNSSGKPKRDFTKLSTPHTYVIIFGVVIFAWILTFVVPAGK
                     FSTQDIEYKDANGETSTRTVLRQDSFRYAYELDKSYVFDQLEELQDHPAEREKLDVPE
                     KGLEKVIADGEKNLTQEKLDEISLTDDVLYDQYGENIYDTSKKLHKTAKIWGTDDFGG
                     FGFLNFVFEGLVSGDKYGSAVGIAALILVVGGAFGIIMRTGAIDAGIYAFISKTKGLE
                     RLALPLLFFAFSFGGATFGMAEEVIPFSMVMVPFVIALGYDSIVAVTVTYVASQVGNA
                     TSWMSPFSVAVAQGIAGIPVLSGATFRLIMWVVVTALAAGYMMIYAEKIRKKPESSLT
                     YKSDDYFRSHIKKTSDENKKFMLGHKLILLEMLVVLVWIVWGVTQKGYYIPEIASQFF
                     VMGLVAGIIAVLFKLDGMKVNDIASSFQSGAADLAGTAIVVGMAKGILLVLGGSDASV
                     PSALNTILHGIGTALTGVPAVIGAWAMYIFQSLFNLVVTSNSGQAALTMPIMAPLADL
                     VGVSRQVAVLAYQLGSGFMDAFTPVSASLIGVLGVARIEWAKWARFQIKMQGFLFVLG
                     TIFIMIAIAIGLQ"
     gene            39283..40467
                     /gene="iadA"
                     /locus_tag="IXT20_000524"
     CDS             39283..40467
                     /gene="iadA"
                     /locus_tag="IXT20_000524"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HAQ4859000.1"
                     /translation="MKIIRQIEVFAPDYLGKMDVLIAGGKILAVEEQLEGGYEGVEVE
                     ELSGEGKVLTPGFIDCHFHILGGGGEGGYQNRTPEVTLSQLTTAGVTTVVGCLGTDGE
                     GRDMTALISKAKGLEAEGISTYVYEGSYRLPVKTVTDSIIKDFLTIDKIIGIGEIAVS
                     DHRSSQPSFEEFARAVADARVGGMLSGKAGIINVHLGGGKRKLELLTRIVEETEIPIT
                     QFLPTHANRTPELFEACVAFAKRGGTIDFTASEDPDFWEKTDGEVRFSKALKRLIEED
                     VSLDNFTMTSDGQGSLPYFDENNHFLGLGVGSAKALLVGIKEAVQKESIPLEIALRAI
                     TSNPARILKLDKKGKIEIGADADLCILDKETLDIDTVIAKGEIMVQEKEVKVWGTFEK
                     SF"
     gene            40578..40781
                     /locus_tag="IXT20_000525"
     CDS             40578..40781
                     /locus_tag="IXT20_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HAQ4859001.1"
                     /translation="MADLKGRSKDMKDKVVGEGKEAYGKATDDKSKETEGKAQSLGAD
                     IKEKARNLGDEVKDKFSRKDEKK"
     gene            complement(40882..41544)
                     /locus_tag="IXT20_000526"
     CDS             complement(40882..41544)
                     /locus_tag="IXT20_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ4859002.1"
                     /translation="MLRTEQLGFWYQDPQDALYKDVELNFEAGKMYAILGASGSGKTT
                     FLSLISGLDAPKEGKVYYQDRSLNKIGLRSYRKNDVSIIFQAYNLLPYLSALDNVIAA
                     MEITKTKQENYRQVALDRLARVGIDEILAKKQVTKLSGGQQQRVAIVRAICCEHQLIV
                     ADEPTGNLDETTSQDIVKLFQEIAHEQNRCIIIVTHEIEVAKSCDEVFELKNKKFQKI
                     EV"
     gene            complement(41557..43059)
                     /locus_tag="IXT20_000527"
     CDS             complement(41557..43059)
                     /locus_tag="IXT20_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ4859003.1"
                     /translation="MNFMKRAWKSTKVKWGRSLLLFAVFTAILIFVLAGLTIRSAAEE
                     AANQAQKEVGATVTLSANREASFKKQEQTSSSGSTTEERPDPGSFSLTPVSLTDAEKI
                     AELDNVKSYSFESSASAMAKDGITAITSSDSSEDSSETTDAGGEPAGGMGGNPQMTQA
                     DFQISGVSATAQNSNFSDGTAKITDGEGIDDSDKGTNNVVIDSTLAETNDLAVGDTFT
                     ITSTEDEDTTYEMTIKGIYESSETSGSMGMKFNFMNPSNTLYTSYTFANELNGTSDDN
                     TIDSAIYTLSDPDKMDEFLKEAEKLIDTDTFSLQSNDSMYQSMLEPLNNVASFSKNVV
                     LLVAVAGIIILTLIIMITIRERRHELGVLLSLGESRSKIILQLFTEVAICMILALGVA
                     SLSGNVVANAVGQQLLDQQTETTTQNQQAPGGGQMPGNRQEQGGQRDGQQGTTNNPFA
                     VSDQVSELEINVKPAQLGLLAGVAFGISLFSVFLASIGILRLNPRKILLN"
     gene            43607..45286
                     /locus_tag="IXT20_000528"
     CDS             43607..45286
                     /locus_tag="IXT20_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HAQ4859004.1"
                     /translation="MSTIKIIPLGGVRENGKNMYVAEVGEDIFILDCGLKYPENELLG
                     IDVVIPDFTYLEENIDRVAGVFLTHGHADAIGALPYLLSKISVPVFGTELTIELAKLN
                     VSRNEAAKGFKDFHVVDEHTEIDFGETVVSFFRTTHTIPDSVGINLKTKEGSIVYTGD
                     FKFDQTAIPMYQTDYARLAEIGKEGVLALLSESANAENPQPVASEAQIADEVFDTIKY
                     WEGRIIVACVASNLQRVQQVLDAAEKAQRKVVLTGQDFERIIRTAMRLEKLQLPSEDL
                     LVTLKDMKNYDPEELIILETGRMGEPIKSLQKMANGTHRNVKIEEGDLVYITTTPSIA
                     METIVAKTEDIIYRAGGTVKLISENMRVSGHANPNDLQLMINLMKPTYFVPVQGEYRE
                     LAAHADLAHAVGMPYKNIYITGRGDVLEYKNKRMSVAGTTSAENVMIDGLGIGDIGNI
                     VLRDRKILSEDGIFVAVVTISRREKKIISKPQITSRGFVYVKASRDLMKESSNMIETI
                     VEKHLESNDFEWSKLKQDIRDQLGRYLFEQTKRRPVILPVIMEATQRRNKK"
     gene            45413..45892
                     /locus_tag="IXT20_000529"
     CDS             45413..45892
                     /locus_tag="IXT20_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HAQ4859005.1"
                     /translation="MMSQTKVERYLEEQKISYQPLDFSHVVEESFTEELQKRGIDPAL
                     ICKTLVVKGNKTGVIIALIPLNDHLDYKKTRQVTKDRKVGFPGMDFVLMHTGYPHGAN
                     TPIGIKLAHPDYCFLADSSLKEKEEVIVSSGEIGRSLRIKVADLEKIVHPIYADLIS"
     gene            46111..46791
                     /locus_tag="IXT20_000530"
     CDS             46111..46791
                     /locus_tag="IXT20_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HAQ4859006.1"
                     /translation="MKITYHGHSCLTIEMENGQRVMIDPFITGNPLTDLTAEEVKTDW
                     LLITHGHSDHIGDMIPIAKENQATIISIVEIADYANSRGVDSFGMNIGGKHAFPFGVV
                     KFVHAQHSSSYEVDGIVQYMGEPSGIIIQAEGKTIYHAGDTAYFSDLGLLAEEFDIDV
                     AFLPIGDNYTMGPEDAAKAAQTIRAKKVVPIHYNTFPVIEQDPESFVELLEDSEGKIM
                     SVSETLEV"
     gene            46799..48130
                     /locus_tag="IXT20_000531"
     CDS             46799..48130
                     /locus_tag="IXT20_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HAQ4859007.1"
                     /translation="MATKHDLILEYIESLPVGNRISVRSIAKELNVSEGTAYRAIKDA
                     ENVGLVSTIQRVGTIRIERKLKKHIERLTFGEVVRIIEGDVLGGAAGLDKVLNKFVIG
                     AMTKHAMERYITPGSLMIVGNRAEVQKLALEDGAAVLITGGFNTTAEIAKLADDLEMP
                     ILRTSYDTFTVATMINRALSDQLIKKDIMLVSDIYMPAEKTHYLQQTDTISDYQRLSE
                     ETQHSRFPVVNRHHRLMGIVTAKDVLGKSPNQIIDRVMTKEPISVKKTMSIASVSHQM
                     IWDGLEVMPVVSDDLTLEGLITRQDVMKAMQLVQRQPQIADTISDQISGNIHTIDTDR
                     EGNRLENPKFKFVVAPQMVNGVGTISFGVLSEIISDVAQKTMVMNQKRNILIEQVNLH
                     YLRLIQLESELDIRPRVLEIGRRSAKLDIEVFIENTIVSKAIVVCQVMERS"
     gene            48145..49107
                     /locus_tag="IXT20_000532"
     CDS             48145..49107
                     /locus_tag="IXT20_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HAQ4859008.1"
                     /translation="MTVQQEIVDTIKKYDKIIIHRHLRPDPDALGSQVGLAELLRESF
                     PEKEIRQVGETIEGLRFLAEMQEVEDVFYQGALVIVTDTANSPRISDQRYQLAEKLIK
                     IDHHPNDDPYGDLVWVNTKASSCSEMIAEFALMFPESLKMNVKAARLLYAGIVGDTGR
                     FLYPSTTSRTLEIASILRNYPFDASALNREIEQMPMKVAKLSGYLYQNIQVDENGAGR
                     IILPQEILDKYGIDDSETASIVSLPGVIDEVLAWGIFVQQPSGYYRVRLRSKGPIINE
                     LAKRHHGGGHPLASGANAKDLAEVEEIYKEIQDICQSYKSRKTE"
     gene            49218..49556
                     /locus_tag="IXT20_000533"
     CDS             49218..49556
                     /locus_tag="IXT20_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkylphosphonate utilization protein"
                     /protein_id="HAQ4859009.1"
                     /translation="MTLPNCPECGSEYTYEDRGLLICPECGNEWNKEEQETVIEDGLV
                     VLDANGNRLLEGDSVTVIKDLKVKGASGAIKQGTKVKNIRLVEGDHNIDCKVEGFGPM
                     KLKSEFVKKN"
     gene            49635..50342
                     /locus_tag="IXT20_000534"
     CDS             49635..50342
                     /locus_tag="IXT20_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HAQ4859010.1"
                     /translation="MYGYGMYGFLDPTFLLVILGLVISGIASAYVNSTFRKYDQVRSK
                     NNVTGTQAAQYILQSQQINNVGVQQIAGDLTDNYNSGNKMLSLSQATAQSTSVAAIGV
                     AAHECGHAVQDASGYVPLKIRAALVPAANFGSMISFPLIMVGVLLSWNSTLINIGIFA
                     FSLALLFQLVTLPVEFNASRRAIQILSEGGLLTEEEVPMARHVLFAAALTYVAAALST
                     FLQLLRLILLFGGNRRD"
     gene            complement(50445..51926)
                     /locus_tag="IXT20_000535"
     CDS             complement(50445..51926)
                     /locus_tag="IXT20_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HAQ4859011.1"
                     /translation="MLIEARKKELLVLQMVIQKNNCSLQELSNDLHIPKRTIKELIRK
                     INVSLKQFLLIDEFIYSNHKGEIQTKETDQTKKLAVFSKLKLCYLKESNRFNYLMLLI
                     NYPQTAVPKKYLLEQLYISPSYLEKLTKQLNHSLKKFHLRILSVKNCCTLEGNELSIR
                     LYLYFFLSDTFGGNDWPFSSLELNALKMNSTDEQIEKISKQEKNIYLLLTLFILRNKQ
                     QAYFPKTSSEITELMDLLQHTHDYSEYFTSFSVSRPCEETETERAYFNYFFYCFFPNQ
                     ISFRQKQEIGFLFSTSKNLICRQVGQLMTYLTQQFPLNHLENKIPIYFYFLVLAVVFF
                     ELLGESSSSFSTLCFPEATSNHSSSFIQEKKLREKIDTIFSKNGERNQISQYLSQLIQ
                     HLLQTETKEQLLIHLQITKQFTGNYHLQSHLQQLFRKDAVKITAYSEHADLIITDQHE
                     KISDETETFYLDTTHNTVLFGKLIKAVHELYMKKGYENKTKRM"
     gene            52235..52771
                     /locus_tag="IXT20_000536"
     CDS             52235..52771
                     /locus_tag="IXT20_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAQ4859012.1"
                     /translation="MMGRVTNYSKEYLLFKSMVYVEEYGMKSITARELAEFCGCSTYP
                     IYTHFKSISGLKEKILEEITVYFENYLAECSSDDVLSIVYLLKDFFSIHESIREIIAK
                     SDLDMDSLFKRPFFSYVKGHTKIKEEKSLELVWLNALGSLRSDDSSQDFLEMMNAVWD
                     KNEVTDEYLENILKEQSP"
     gene            52849..54219
                     /locus_tag="IXT20_000537"
     CDS             52849..54219
                     /locus_tag="IXT20_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NOL1/NOP2/sun family putative RNA methylase"
                     /protein_id="HAQ4859013.1"
                     /translation="MKEEATTLPQQFIKKYRLLLGEEASDFFSALEQGSVKKGFRWNP
                     LKPAGLDMVQTYHSEELQPAPYSNEGFLGTVNGKSFLHQAGYEYSQEPSAMIVGTAAA
                     AKPGEKVLDLCAAPGGKSTQLAAQMKGKGLLVTNEIFPKRAKILSENIERWGVSNAIV
                     TNHAPAELVPHFSGFFDRIVVDAPCSGEGMFRKDPNAIKEWTEESPLYCQKRQQEILS
                     SAIKMLKNKGQLIYSTCTFAPEENEEIISWLVENYPVTIEEIPLTQSVSSGRSEWGSV
                     AGLEKTIRIWPHKDQGEGHFVAKLTFHGQNQMHKEKKTRKKSKVQMTKEQEKLWTEFS
                     NDFHYEATGRLLVFNDHLWEVPELAPSLDGLKVVRTGLHLGDFKKNRFEPSYALALAT
                     KKIENIPCLPITQKEWQSYTAGETFQRDGNQGWVLLVLDKIPVGFGKQVKGTVKNFFP
                     KGLRFH"
     gene            54286..54621
                     /locus_tag="IXT20_000538"
     CDS             54286..54621
                     /locus_tag="IXT20_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4859014.1"
                     /translation="MKTSTKVTIGLSVAAAASVATAVVVSGKVMEKIHHMTTRTKVKK
                     FVYDKFDGNEKLLEIVDGLSDSDLDSLMGMLAKIKSGKKKISVYGDSIKDSTEDVKDR
                     LMHFVEKMM"
     gene            complement(54702..55559)
                     /locus_tag="IXT20_000539"
     CDS             complement(54702..55559)
                     /locus_tag="IXT20_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fis family transcriptional regulator"
                     /protein_id="HAQ4859015.1"
                     /translation="MKMDKLLALYPQAQKKNSPASEADILSFAIDDAFIWIKQDSLSQ
                     QETSLLKALFPVINDQKKHPWYHYLFKDASCVQEKSFRLIQLHVESKGEFLKKEWQDT
                     ILELFRYSADFFFYTDTDALLVEQINRDYLDATEINGIFLSLDADFDTTTSVFVGSFH
                     SPGTDLVPLFAEERKIFLTEQNNLYTHSRTFSMQDVALHYYTKDTVKDSKLIKSYIEL
                     IKQNEMEVIIQELWNELGNVSSAAKTLFLHRNTLKYRIEKFQEQSGFNLKKANDLLFC
                     YLLLLHEHN"
     gene            55666..56181
                     /gene="tadA"
                     /locus_tag="IXT20_000540"
     CDS             55666..56181
                     /gene="tadA"
                     /locus_tag="IXT20_000540"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HAQ4859016.1"
                     /translation="MVNTNLDAVEKEKWMRLALAEAKKAEMLHEVPIGAVVVLDGKVI
                     GRGYNLRETTQDATTHAEMLAIKEACEKVGSWRLEDAALFVTLEPCPMCSGAMILSRV
                     AEVYFGAYDPKGGTAGTLMNLLEDERFNHQAYVEGGILEDECGMILTDFFRKLRQRKK
                     KLKKDWSTNEK"
     gene            56204..56288
                     /gene="ffs"
                     /locus_tag="IXT20_000541"
     ncRNA           56204..56288
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="IXT20_000541"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            56498..57883
                     /locus_tag="IXT20_000542"
     CDS             56498..57883
                     /locus_tag="IXT20_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HAQ4859017.1"
                     /translation="MEKIVILHTNDLHSHLENWPRIRRFLDQRKRENEKKEKTTTIAV
                     DLGDFVDRWHPLSEATNGQENILLMNEVGYDAATIGNNEGVGNSKEELNHLYDHAEFD
                     IILDNLFDKNTLQPPAWTKPYKIITTKHQTKIGLIAFTAPFPLTYNPNGWDIRNPYDI
                     LPELVEDLRSQVDVLVLMSHLGIQDDFQIAKEIPAIDVILGSHTHHLFREGHVINDVQ
                     IAAAGKYGQYVGEVHLTIDEEKKILKHSAKAIPTETMTAFTEDEQEINSYLERGHELL
                     KEKEVADLPFSLSLDIFNEHSFIQVAFEAVKERGKTEAAILNSGLFLTEIPKGRVNQD
                     QLHTALPHPMHLLNVTLGGNDLIRLVLEIEKNRNFLRNYPMKGMGFRGKIFGQMVYNG
                     ITYDSINHQVFWKNKPIDLNKNYTFTTVDHLMFVPFFPTIEIAGKNEFLFPEFIRSVV
                     GDYLKAHYPIK"
     gene            57920..58597
                     /locus_tag="IXT20_000543"
     CDS             57920..58597
                     /locus_tag="IXT20_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YutD family protein"
                     /protein_id="HAQ4859018.1"
                     /translation="MSAKQNEQQNVEHKTEIAKKKSSAEQETTVTEELTAALEEIVEE
                     PKAEKVKGELVTLLDDSNFLIGERHYRLVSDYREGFNAEKLGERYSDVLARYDYIVGD
                     WGYEQLRLKGFFQADNRRAHPDQRIDSLEDYLYEYCNFGCAYFVIERIGGKKEKTTQR
                     RKKKKNHNRAYIDEKKGPVASNRKKPVIKNRKTESAKTSEKVKKQEQPKQVEKTKTAF
                     TIRKREE"
     gene            58605..59369
                     /locus_tag="IXT20_000544"
     CDS             58605..59369
                     /locus_tag="IXT20_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01457 family HAD-type hydrolase"
                     /protein_id="HAQ4859019.1"
                     /translation="MDYKGYLIDLDGTIYRGTQPIPAGKRFVEELQKRKLPFLFVTNN
                     TTKSPETVANRLADEFDIHVAPETVYTATLATIDFMKADGKGKKVYVIGEAGLIDLIL
                     AAGFTWEEEAPDYVVVGLDNYLTYEKVVKATLAIQKGATFIGTNPDKNIPTERGLLPG
                     AGSVISFVETATQTPPIYIGKPEAIIMDKAVEVLGLQKEEVIMVGDNYETDIQAGIRN
                     NIDTLLVLSGFTKEEDVPGLPIPATYVKQSLDEWSF"
     gene            59371..60030
                     /locus_tag="IXT20_000545"
     CDS             59371..60030
                     /locus_tag="IXT20_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HAQ4859020.1"
                     /translation="MKNSRWQWMEYAGLFSLFLTLISLAVGVTINFRPLYVFDIGHLQ
                     ILDYTSLDQETLLKNFDHLMNYLNNPFKTILSLPDFPVSASGAHHFYEVKILFLVDYA
                     VFFITLIPSILFIKYLQKNDRLWRLIRPFQIGMLLPVVFGFFMMIGFDRFFILFHETF
                     FNNDDWLFDPVTDPIINVLPEEFFMHSFILFFVLLELFFAVFLFLGKNSLKQTKKKEL
                     V"
     gene            complement(60060..60569)
                     /locus_tag="IXT20_000546"
     CDS             complement(60060..60569)
                     /locus_tag="IXT20_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HAQ4859021.1"
                     /translation="MVVKTETLEEKARELLKERGVTVEDIAQLVMFLQKDYISDLTLA
                     DCTESVNSVLTKREVHNAIITGIQLDILAEKKKLMSPLQHIIEDDEGLYGIDEILALS
                     IVNVYGTIGFTNYGYIDKVKPGILKELNSHDGVHVHTFLDDIVGAIAAAAASRLAHKE
                     PEKRSRLTQ"
     gene            complement(60681..61679)
                     /locus_tag="IXT20_000547"
     CDS             complement(60681..61679)
                     /locus_tag="IXT20_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAQ4859022.1"
                     /translation="MEIYDITIIGAGPVGMFAAFYAGMRQAKTKIIDSLPQLGGQLAT
                     LYPEKYIYDIPGYPAIKASELIDQLEKQLTTFNHTFHLKEEVLSLTREDEVIEITTNK
                     GIHYSKAVILALGNGSFQPRKLNLDNAESFENHGLDYFVNDLMKYAGKKVAIAGGGDS
                     AIDWTLMLEPIASEVYLIHRRPEFRAHEHSVSRLKSSSVNLLTPYLIDGLSGNNGELT
                     DIRLKKVKSDETIDLMIDSLIVNYGFSSNLEHLSSWGLDSTRNAITVKSDMSTSIPGV
                     YAAGDICTYEGKVKLIATGLGEAPTAVNNALHYINPKERTQPGHSTSLYDKNLRPS"
     gene            61880..62464
                     /locus_tag="IXT20_000548"
     CDS             61880..62464
                     /locus_tag="IXT20_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAQ4859023.1"
                     /translation="MAFPQLDLKSEKGPKAVIKTNRGDITVQLFPELAPKTVQNFIEL
                     SKKGYYDGVIFHRVIPDFMIQGGDPTGTGMGGESIYGESFEDEFSRELFNLRGALSMA
                     NSGPNTNGSQFFIVNNTNVPANMLGQLEGAGFPSEIIEAYKGGGTPWLDFRHTVFGHV
                     LEGMDTVDEIANVQRGPQDRPVHDVVIETIEISE"
     gene            complement(62541..63716)
                     /locus_tag="IXT20_000549"
     CDS             complement(62541..63716)
                     /locus_tag="IXT20_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAQ4859024.1"
                     /translation="MIEFDKQISRKDTESVKWDAIAETYQMDDLLPLWVADMDFLSPG
                     GVAKAFSEYIKHGIFGYATLSDKLYQAIIHWERQHHAVELTKENIVFTSGVLTSLAAA
                     VQTFTKPGDSILIHDPVYPPFSSIIETNQRHIVRSSLLEKNNHFIMDLSDMEKKIEEN
                     NVKAMILCNPHNPGGRVWSKEELKQLSELCLKHQVILFSDEIHQDLTLFDHTFTSMLT
                     IDPNLDDLLVAFTSATKTFNLAAIKNSMVFIKNPEMKADFEKHLVMNQQHEINTFGLI
                     GTQAAYETGGEWLSQLIPYLEKNVQTVNQFFEQYLPKVRIMNPEGTYLMWLDFTAYIS
                     DDQELENTLVRKGKVVLNPGITFGPSGHGHMRLNIACPEETLLEGLNRIKYALETID"
     gene            63798..64178
                     /locus_tag="IXT20_000550"
     CDS             63798..64178
                     /locus_tag="IXT20_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HAQ4859025.1"
                     /translation="MTYKIGQIIEGKVTGIQPYGAFVSLDNETQGLIHVSEVQSGYTK
                     NIHSLLKVGQPVTVQIIDIDEYSKKISLSLRTLEKTNPAVPYRRKRYFTNKNRKIGFT
                     TIAEQLPIWIEEALSELERKENKK"
     gene            64252..64614
                     /locus_tag="IXT20_000551"
     CDS             64252..64614
                     /locus_tag="IXT20_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4859026.1"
                     /translation="MASKRVAGTIMLHLEDGTKKFLVHSIDDKLEFALAELSEGKTGL
                     ANILNFLKEIVHIDVSKISLMELTNTHIKEENVPLFVFETEQKNQREELGEGYIWEEP
                     GQMRSVLGTYEVEGVPFF"
BASE COUNT        21219 a        11184 c        13379 g        19165 t
ORIGIN      
        1 tcagataagg gactgtgaca agacttttgt cacagtttct ttttgtttat tcttttttat
       61 ctttgtatgg caataataga caatataacg aaaattattt aatggctttt ggaagaccat
      121 ggaaaacctt gtacaaagct attttttctg aaaaattcct tgaaacctca cgaaaaaatc
      181 attattttat gaaaaagaca aaaaaaagtg ttgatttttt aataattatt cattaaactc
      241 ttaaacaact tcagaaaata ttaattattc tgaaaattcc aataaagggg tacgtaaaat
      301 gaaaaagaaa tttacatttg gtttggttgc ggtatgcggc cttgtacttg ctggatgtta
      361 tggcggcagt tcagccacaa gcagtagttc agcaaaaagc ggttcagcag atggaggagg
      421 cgtatttaac ttagtcgtgc ctcaagaaat gcctacagct gacttatcag ttgcaacaga
      481 tacaatcagt tttacagcat tgaataacgt ttatgaagga atttaccgtc ttgatgaaga
      541 cagcaaacca cagccggcag gagctagcga gttggcagaa gtcagtgaag acggattaac
      601 ttacaaaatc aaattacgag aagacgcaaa atggtcaaat ggtgatcctg ttacagcagc
      661 ggattacgtt tatggctggc aacgaacagt agacccagct acagcttctg aatatgctta
      721 cctgtatgcg ccagttgaaa atgcagaaga cattacagct ggaaagaaag ataaatcaga
      781 actagggatc aaagcagttg gtgactatga attagaaatc aaattgacga aacaaacacc
      841 atatttccaa tatctgcttg ctttcccatc atttttccca caaaaacaat ccgtagtgga
      901 agaaaatggg gatgcctatg catctgcaag tgataaagct gtttataatg gaccattcac
      961 attagaagat tttgatggac caggaacaga tacagaatgg acttataaga aaaatgatca
     1021 ttactgggac aaagatacag taaaactttc tgaaatcaaa gtcagtgtag taaaagaatc
     1081 ttctactgca ttgaacttat tcaaagatgg ccaagcagat gacgtgatct tgtctggtga
     1141 gttagcacag caaaacgcaa acgatccagc ttatacttct gttaaagaag cgcggacaag
     1201 ctatatcgaa ttcaatcaac gtgaaaagaa ttcaccattc aacaatgtga acctaagaaa
     1261 agctatttct tattctatcg atcgtgaagc tttagtaaaa caagtactag gtgacggatc
     1321 agttgtctct acaggattga tcccatcaga tatggtaaaa agtccaacaa cgaacgaaga
     1381 ctttgcaaaa gaagctggaa aattagtttc ttatgacaaa gataaagcaa aagaatattg
     1441 ggaaaaagcg aaaaaagaat tgggcgttga ttctttagaa tttgatttga tggcttctga
     1501 tgatgattca tcgaaaaaag taatcgaata tgttcaaaac tcaatccaag aaaacttgga
     1561 tggcgtaaca gtcaaaccaa cacctgttcc attctctgta cgtcttgacc gctcaacttc
     1621 gggtgatttt gacgcagtac taggcggatg ggctgctgac tatgcagacc caagcagctt
     1681 tactgatttg tttgtcacag gtaattcata caaccgtggt cgctggagca atgcagaata
     1741 tgacgaagca gttaaagcag cttcatcaac agacgctggc gatgaacaag cccgttggga
     1801 tgatttccaa aaagcaatca agatcatctc tgatgatatg ggtgtcgttc cggtgtacca
     1861 aaaagcagaa ggtcacctag tgaatgaaaa aatcaaaggt atcgttcacc atgcggcagg
     1921 cgcttcatgg gattacaaat ggacatacgt tgaagaataa tcgttaatac cgtgagcatg
     1981 agaaaaaacg aggctgcggc aaatgtcgca acctcgttct catattcact tatcctaaca
     2041 aagaaaaaag catgaaaata gcagaaagag caattagtat tccggcaatg atcagccaaa
     2101 agctgtacat accgtagctg gcagacgaga gcgaggaaaa ttctggtttt ttctttcggt
     2161 tccttgttcg tgcaagatgg accgatcgct gaaaatgatc gaagaaaccc gaagaaaaga
     2221 cccataaaaa accgccgata atcagaaacg gcaatgacca taagaataga tcattagata
     2281 attgaatgag tgtaaaggaa tggttgatga gattttttat taaaataacc agaatcagta
     2341 gaaaagctac tgcgtagggg atagattttt ttttcatttg attgctcctt tatttttggt
     2401 tcattatcta ataatgtagc gaaacatctg tcagaagtca aaagctaggg ggaaacatta
     2461 gtgaatagtt atatcaaata tgtattaaaa cgtgtctttt tcatgatcat cacgctttgg
     2521 ttaattgcaa caatcacctt tttcttgatg caattgctgc cgggcacgcc atatacgaat
     2581 caggaacgtt tgagtcctga aacaatcgag atgctaaata aacaagtcgg tttggataaa
     2641 ccagttatcg tacaatatgg gatttatctt tctaatttgc ttcaaggaga ttttggaatt
     2701 tccttccaat ttaaaaacca gccagtcgca catttgttag ctggaagaat cggaccatcc
     2761 ttgcagttag ggttacaggc aattatcttt ggaacagtcc tagggacgat tttaggaacg
     2821 atctctgcca tgaagcaaaa tacatgggca gatacatctt ctacactcgt agcgatatta
     2881 ggtcgttcca ttccaaactt tgtatttgct gttttattac aatatatctt tgcaatcaaa
     2941 ttacaagttt tgccaatcgc aaaatgggat ggctttgtgt acacaatctt accaacaatt
     3001 gcgttggcca tgtcgccgct tgctgactct gctcggttta tccgtacgga aatggtggaa
     3061 gtactgcata gtgattatgt ggaattagca aaagcaaaag gattgagccg ttgggaaatc
     3121 gcctttaaac atggtcttcg aaatagtttg attccattga tgactttatt aggaccgtta
     3181 gctgtagcat tgatgaccgg atcactggtg gtagaaaata tctttgctat tcctggtatc
     3241 ggggaacaat tcgttaagtc aatcacaaca aatgattatc ctacgatcat ggctgtaacg
     3301 atcctttact catttatgct gatttttgtt attttgatcg tggatctatt gtacggtcta
     3361 gttgatccaa gaattcgtgt ttcagaaggg agccgaggat aaatggaaat gataccaaag
     3421 cgttatgaaa cagttgcaga tatccctgcg gatgaatttc ttccattaca aaaaaatacc
     3481 gaagaagaac gtgaacagat tactgcacct tctttgaatt ttatccaaga ttcatggcgc
     3541 cgtttaaaga aaaacaaagc tgccgttatt tcaatggttt tactattggt catcattttg
     3601 atctctgtga tcacaatctt tgtttctcct catgatccaa ctgcccaaaa cgtggcttat
     3661 atcaatttgc ctcctcgtat tccgggaatc aatatcgatg gattgaatgg aaaagcaatg
     3721 gttggtggtg aacttgttga taaatatgct caagcaaacg tcccttcaaa cgtaaatttc
     3781 tttttaggaa cagacggttt gggacgtgat gtattaagcc gcttatttat gggaatgaga
     3841 atctcgttgc tgatcgcatt tattgctgca ttactagatg taacgattgg tgtagcttac
     3901 gggttggttt ctggtttgtt aggcggacgt gtcgataacg tgatgcaacg ttttttggaa
     3961 atcttatcag gaattccaaa cctcgttgtc atgattttga tgttggtcgt ttttgaacca
     4021 ggaatttttt caatcgtggc tgctatggcc atcacaaact ggataccaat ggcacgtatc
     4081 gttcgtgcac aaacaatgaa attaaaagat caagaatatg ttttagctgg attgacatta
     4141 ggtgaatcaa gaatgaaaat tgcattcaaa catattttgc cgaatatctc tagtgtcatt
     4201 attattcaaa tgatgtttag tattccatct gctattttct tcgaagcctt tttaagcttt
     4261 atcggtttag gtcttacgcc tccgtcagct tcattaggaa cgatgttgag cgaagggtac
     4321 aagacattcc tgtatctgcc ttatctatta tggattccgg cagtaacttt atcggttatc
     4381 atgattggct ttaacttact tgctgatgga ttgcgtgatg cctttgatcc taaaatgaaa
     4441 gagtgaagac gatgacgaaa attttagaag tgaaagactt agaaatctct tttgatacgt
     4501 atgctggaaa ggtacgagcg attcgcggtg tcaattttca tttgaataaa ggagaaacat
     4561 tggcgatcgt tggggaatct ggaagtggaa aatcagtgac gaccagaagc atcatgcgct
     4621 tgctgtcaag caacgccaat atagatgctg gtcaaatcct ctttaaaggt aacgatatcg
     4681 ttggaaaatc agaaagagag atgcaaaaaa tccgtggtaa agaaatagcg atgattttcc
     4741 aagatccaat gacctcttta gatccgacga tgcccattgg gaagcaagtc gctgagtccc
     4801 ttagaaagca taataaagtt tcaaaaaaag agggacaaaa agctgcatta gaattgctta
     4861 aattagttgg aatacccgaa gctgagaaac gtatcaacag ttatccgcat cagttctcag
     4921 gaggtcagcg tcaacggatc gtgatcgcga ttgcactggt atgttatccg gaaatcctga
     4981 ttgctgatga acctacaaca gcgcttgatg tgactattca agcacagatt ttggagctgc
     5041 tgaaagaaat tcagacaaaa atcgatacat caattatctt tatcactcat gaccttggtg
     5101 tagtagcgaa tgtcgctgat cgtgtcgctg tcatgtatgg aggaagaatc gttgaagttg
     5161 gaacatcgga agaaattttc tacaacccgc aacatcccta tacttgggga ctgcttggtt
     5221 caatgccgac attagatagt gcgaacgatc gattgtatgc tattcctggt tctccaccgg
     5281 atttattgaa cccgccaaaa ggagacgcat tctatccaag aaatgaattc gcgatgaaaa
     5341 tcgatgcaga acaagaacca ccattttttg aactatcaaa aacacacaag gcagcaactt
     5401 ggctacttgc tccgcaggca cccaaagtga caccgccaga agaaatccaa cgtcgctggg
     5461 ctatctttaa agagaaacaa aaacatactt acggtcaagg aggaattgct gatgccaaat
     5521 aaactacttg aagttaaagg attgaaacag tatttcaatg taggaaaaaa gaatgaagta
     5581 catgctgtaa atgacatcag ctttcacatt tatgaaggag aaacattcgg tttagttgga
     5641 gaatctggaa gcggaaaatc cactactggc cgtacgatca ttcgcttgaa tgaacctact
     5701 gatggtgaga tattattcga cggtcaagat ataacgaaga tcaaagataa aaaaggtttg
     5761 acgaaattcc ggcatgatgt tcagatgatc tttcaagatc catacgcttc gttgaatcct
     5821 cgtatgaaag tcagagatat cattgccgaa ggaattgatg ttaatggact agctaaatca
     5881 tctgaagagc gtgcaaaaaa agtcgatgat ctattgagaa ctgttggatt gaatccaagc
     5941 cacggtactc gttatcctca tgaattttca ggtgggcaac gccagcgtat tggtattgca
     6001 cgagcattgg cagtaaaacc gcgttttatc atttgtgatg aaccaatttc tgcactagat
     6061 gtgtcgatcc aagcccaagt ggtcaatttg ttgcaagatc tccaaaaaga acatcaattg
     6121 acctatctat ttattgcaca tgatttgtcg atggttaaac atattagtga ccgaatcggt
     6181 gtaatgcata acggcttgtt attagagatg ggaacaagtg atgaaatcta taatcacggt
     6241 gttcatcctt atacagaaag tttgttatct gctattcctc taccagatcc agaccatgag
     6301 agaaaacgca gaagaatcaa atatcaacct gaaccagatg acggacaagt acgtcaactt
     6361 cgtgaaatcg caccagaaca ttttgtctat gcaactgagc aggaagtccc atattacgca
     6421 aaaaaactaa aacgacaaaa ggaatctctt ctggttgcta actaggaggg acatagatgc
     6481 taaaagcttt taaattccgt atgtatccga cagaagaaca aaaacagcaa ttgattcgta
     6541 cttttggatg tgctcgattt acctataacc atttgctgaa aaagcggcag aaaagttggc
     6601 aacaaacagg agtagccaat ttttcgctca cacccgctac attaaaaaaa gaatacccat
     6661 tcttaaaaga agtagacagt cttgctttag caaatgcaca gcttaatttg gatcgggctt
     6721 ttcgcaatta tttcaaggga cgtgccagct tcccaaagct taaaacgaaa aaaagtatgt
     6781 ggcaatctta tacgaccaac aaccaaaccc gtacgatcta tctggaaaat ggctacttaa
     6841 agttaccaaa acaaaaagaa ctaatcaaga taaatcagca tcggccagtt gaaggatcga
     6901 ttcgctccgc tacgatttct gctcgatata atgaagaatt ttatgtcgca ctactttgtg
     6961 atgtttcgcc agtaaaaaaa gaatctttag ctaaatggat cgggatcgcc tatcatccga
     7021 agacattgat ccaaacatct cgacctctag aagtaacttt accaaaattt caccagacag
     7081 aagaaaagct gcaacatgca cagcggaaat tgaatgtgaa agtccgctcc gctcaccata
     7141 gaaagatcag gttagatcag gcaagcaatt atcagaaaca aaagagaaaa gtaatggatc
     7201 tttatctgaa acaaaagaac cagcgagaag attatcttga acaattatct gggaaattgg
     7261 tcaaacaata tgactactta tttgtagaat cttttccaaa agaagaggca catgctgatt
     7321 tttctattca cgactggcat aaattaataa caaaattgcg ttataagtcc caatggtata
     7381 ataaaaaatt tcttttgatc aataccgacg gagcagaaga aagcaattcc gtaagaaaga
     7441 gccaagtagt agaaaaaatg ggcagacatt cggttatcaa gggataaaaa atgaatgaaa
     7501 ctgataggag cgttagggtt agccttgtta ataaaaagtc ttaatgactt ttgtttctag
     7561 gaatttttct tttgaagaaa agctcactaa ttatgacaaa ccaataaaac aatagcagaa
     7621 aaagttcgta cgagcttttt ctgctattgt taatttttta ttcagaaagt ttacgaatac
     7681 atcaaaattc tgaaacaaaa aacgtaactc acaaagagtt gtttttgcgt tttttcttga
     7741 atgattaggt ctgaatcttt tgctcacaac cttattcatg gtgccccggc ttgagctgat
     7801 agatttttga aggacggccg attcctacag gacgttcacc gatctcatcg aaatattgga
     7861 gcatagcttt cttaaaattg gaatgatcga tcgttttata atcgatccct aagaatttag
     7921 caaatacttt tcgagcttcc gtaatcgtga aatctttacc taaaacttgt agaacttgcg
     7981 gctcgtgttc cattttattg actacacggt tgaatgcttt caagatgatt tgactatggt
     8041 caaaagccaa agtatctttg ccagaagact ctccagtgac taaatcaagc gagatagcca
     8101 cttctccgct agataaatgg atcttgttac tatgacgttc tagtgtgaac cagcgtactt
     8161 ccttggcatc atctccggcg attagcggtt cttctccaat aaaggctaag tagctgactg
     8221 tgacgaccca gcctcgcgga tctcgattag gcgtactgaa tgtatgcagt tgctcaatat
     8281 tttcttttga aataaccaca ccagtttctt cctttgtttc tcttaagacg ctttctccag
     8341 ttgactcatc cttttggaca aagccaccag gcaatgccca tgaatttcga taaggatgcc
     8401 cttttctttg gatcaacaaa actttcagct gatcttcttc tttgttatag cacatcagta
     8461 cgatatcgac agtcaaagag ggcttgtcat attctggacg ttcctgtttt tgataccagt
     8521 taagaaattc ttcttcactt gctttatgtt cataatataa tttttcttct tctttagaag
     8581 caaaacggtt catctgacca cctccattga cagtaaaagt actttttacc ttattgtagc
     8641 agactctgaa aaaaaaccaa ggatttactt gaaaaatcta ggttatcatt taaatatgaa
     8701 aaataaatgg aagtatgaga ttgaataaaa agaaaagcag ataaaacggc aatactttat
     8761 accgctcttc acgaatggca aagaagccac aaataacgat atatacgtca atgataaaac
     8821 gcaaaatcca agctgctgtc ataaaactgt tcaaatgccc atggaggtta aaataaccat
     8881 acatacgaat ataaaaatag ctgaatatta aatgtgctaa tgaaagaaaa gcaatactaa
     8941 gtgtaaaaaa aagatgtttc ttctgcatga ttgttccagt cccttactaa gatagatagt
     9001 ctttattata ccacgtgtga ggggagaaat aaaaagccgt tttttagtag atatgaaaca
     9061 aaacaaaaac ccgcatttat tcaacctatt catcctttta aaggatagat tgaacgtaga
     9121 tagaagtcgt ttatccagtc tttttcattt tgaaaaaaag ctaacagcat gcttttttta
     9181 tggtatacta gcaaggaaac gaattcgcat catgcgataa aggaggaaga gccccgtggc
     9241 agaaaaggaa actttttata tcactacgcc aatctattat ccaagcggca aacttcatat
     9301 cggcaactcg tatactacga ttgcatgtga cgcaattgct cgttataaac ggttgatggg
     9361 ctttgatgtg ttttatctca ctggagtgga tgaacatggt caaaaaatcg aaacaaaagc
     9421 agaagaacta ggtgtaaaac cgcaagaata tgtagataaa atggcggctg atgtcaagaa
     9481 attatggaaa acgcttgata tcagttatga taaattcatt cgcacgacag atgattacca
     9541 taaagcagct gttcaaaaaa tatttgatcg tttattagaa cagggagata tttacttagg
     9601 tgaatatgaa ggctggtact ctgtttctga cgaagaattc tttacagaaa cacaacttgc
     9661 agaagtgtac aaagatgacg aaggtaacgt gatcggtggt aaagcgccaa gtggacacga
     9721 agtagaattg gtaaaagaag aatcatactt cttccgcatg agcaaatacg cagaccgtct
     9781 attggaatac tatgaagaac atcccgaatt catccagcca gaatctcgta aaaacgaaat
     9841 gatcaacaac tttatcaaac caggattaga ggatcttgca gtttcacgaa caacttttac
     9901 ttggggagta ccagtcaaga gtgatccgaa acatgtggtg tatgtatgga tcgatgcatt
     9961 atccaattat attactgcac taggatatgg ctccgaagat gaaagtctat tcgaaaaata
    10021 ctggcctgca gatgttcaga tggtcggtaa agaaatcgtt cgctttcata cgatctattg
    10081 gccaatcatg ctgatggccc ttgatttgcc tctacctaaa aaagtatttg ggcatggctg
    10141 gctattgatg aaagacggaa aaatgtctaa atcaaaagga aatgtcgttt atcctgaaat
    10201 gcttgtggaa cgttatggtt tagatgcact ccgttattat ttgcttcgtg ctgttccgtt
    10261 tgggtcagat ggtgtcttta cgccagaaga ttttgtttcc cgtgtcaatt atgatctggc
    10321 aaatgatcta gggaacttat tgaatcgaac gattgctatg atcaacaaat attgtgatgg
    10381 actcgttcct gactatgcct cacttgtcac tccatttgat agtgaactgt ctacgacggc
    10441 tgctaacgtt gtcggacgtt accatgatgc aatggaaaaa atggaattca atacagcatt
    10501 ggctgaaatc tgggtgctga tttctcgtgc aaacaaatac attgatgaaa cagaaccatg
    10561 ggtattagca aaagacgaag aaaagaaagc tgaattgaat agtgtcatga tccatttagc
    10621 tgaatctttg cgtatcgcag cgatattact tcagccaatc atgacagaaa cgccagagaa
    10681 gatcttcaat caactaggat tggactctga aacaatgaat cttgaaggat tacactttgg
    10741 cgaatttcct tcaggaacaa aagtagttgc aaaaggtaca ccaatcttcc cacgtttaga
    10801 tatggaagaa gaagttgctt ttattcaaga aaaaatgtct gaaggtactc aaacaaacga
    10861 agatacagtc aaatgggatc cagaagaaac agaacttgtt tcaacgaaag aaaaacaaat
    10921 caaatttgat gtatttgaaa aagtagaact aaaagtagca gaagtcatca actgtcaaaa
    10981 agtagaaggg gcagataagt tgctgcaatt ccgactagat gctggagaca gccaagaccg
    11041 tcaaatcttg tcaggaatcg ctgaatttta tcctgatcca agcgaattga tcgggaaaaa
    11101 agtcgtcatt gtagcaaatc tgaaaccacg aaagatgcgt ggacaaatca gtcaaggaat
    11161 gatcctttct gcagaagcac cagatggctc tttacaagta atcgaagcac caaaatccat
    11221 gccaaatggc tcagaaatcg catagaaatt caaagggggt atgacactag ttgtgtcata
    11281 cccccttttt ccgaaatttg agaagcaatt ttcgtaaatt ccttcttatt acttgaagcc
    11341 aatcatataa tacagaaaca gagaactatc caaaataaat cctagaaaaa gaagtacgag
    11401 cttcgctgat ttttgttaat ttagaataat actaatagga atcgtaaaaa cagaaaaaaa
    11461 gcataatgat aaagggtcgg gcagatttta attgattcat ctggtactct tttttattgt
    11521 ccttacattc ataaagtttg aatgcaatga ctttattaca tctatattta tatctaacag
    11581 tagcgttgat gtgttaatta taagttctat cactttatgt tcaattagtt tgtgctctta
    11641 tatgtgtgat tgaaaatata gataaaaaaa caatcatcgg ctttgacttc gatgattgtt
    11701 ttctttattt tgtaaaaatc ctatcacttg attttatggt aaccttttat ttttgtcttc
    11761 aaacggcttg gtaagagagc agtaagcagt gcaaatggga tcgaaaagac ataccagaaa
    11821 atcgtacgtc cgataccata atgttgatag agctttgtca acatcacgtg taacaagatc
    11881 aaccctaaaa gcaaggagcc agctataaac caatgattat cccaatgcat gacttcacta
    11941 agtgccatca ctccaaaagg atagaaataa aaatagatcc cgactaaggt catccgccag
    12001 ttctttgtaa atgttccgat attcaataaa aagaaaatcg ctgaaagcaa aataccgata
    12061 ggcggaaaaa gcccaaccaa aaaggaatac aacatccccc acaagatcat cacaggacct
    12121 ttgatcaatg caacgattgc gatgaacaaa gtcagcaata ttagtttttg tgataacgtt
    12181 gcaacagtca ttaatccaaa taaaattaat gtgaaccaat gccaatggga ctgctgttgt
    12241 tctttacggg aaaaatcgac ccggttggat aaataatctg ctaatttcgg ataagaacgc
    12301 ttaaaatcca taaactgttc ctccttcgtg gaaaaggtag gtttagtata cgaaaaataa
    12361 aaaagaaata catccgtctt aagagggagt ttttcactga aagtggagaa aaaagaatgg
    12421 taaattaaaa tattctcagt tatactgaaa attataccaa atttcaaaga agaaagagga
    12481 tgggaatgaa aggcaaacta aaacgagaaa tcaatctgtt tggcgcatta gctaccgtta
    12541 tgggaaccgt gatcggtgca ggcgtcttct tcaaaactgc tgctgttact gctagtactc
    12601 agtctacaag tttgacattg ctagcttggc ttttgggtgg tttcttaacg atctgtgctg
    12661 gattgacagt ggctgaatta gctacagcaa tcccggaaac tggtggagct gtaaaatata
    12721 ttgaagctgc gtatggaaaa ttgcccagct ttttattagg atgggcgcaa agcttgattt
    12781 actttcctgc taatattgct gcattatcga ttatctttgc gacacaattg acgaacttat
    12841 tacaattatc taccgattat cttttgttga ttgcagtgat caccgctgtt tctgtgacag
    12901 ggttgaattt gttagggaca aaagtcggtg cttctgtaca atcagtgacg ttgattgtta
    12961 aattaatacc aattgctgtg attgtcatat ggggattatt gaccccagga caaggaactg
    13021 tccagttatt tccaatcgaa gctggaaaag atgttacttt tgtcgaagga ttgagcagtg
    13081 cgttgttagc aacgttgttt gcttatgacg gctggttagg tgtcggtgca atggcagggg
    13141 aaatgaaacg gccggaaaaa gatttgccta aagcaattat tcttggtttg agttttgtaa
    13201 cagtcgttta tctattgatc aattttgttt ttttgaaaac tttgccgatt gatcatcttg
    13261 ccggtaattt gaatgcagct tctgaagcat cagacgtcat ttttggagga atcggtggaa
    13321 agctggtcac tattgggatc ttgatctcgg tatacggtgc attgaacggt tatacactaa
    13381 ctgggatccg cgtgccttat gcgatggctt tagaagacga gctacctttc agtaaacagc
    13441 tgacaaagct ttcaaagaag tttacggttc cttatgttcc tgcggttttc cagttagcag
    13501 tagcttgtat tatgatgagc cttggttctt tcgacttttt aacagacatg ttgatttttg
    13561 tgatgtggct attcagttta ctgattttta tcggtgtctt tattttaaga aaaaaagcgc
    13621 cggaattgcc acgtccatat aaagtaccac tttatccaat cgttccgatt attgcgattc
    13681 tcggtgctat tttcatttta ggtatgacga tgatgacgca aacgaaatta gcattgatcg
    13741 gtataggtgt taccttgata ggtatcccag tctattatca aaaaaagaaa aaacgctcag
    13801 aagagtaaaa tatttcaaca acagacagaa aaagtacgaa ctaggtcctc tagatcttcg
    13861 tgctttttct atttcaataa aacaagttct tctattttga tttttgctac ttctgcaagt
    13921 agtgcttatg tgctaaagta agaatgaaaa ggagagataa acatgatatt tgattctcat
    13981 acccatttaa atgctgaaca gttcaatgaa gatattccag aaacgattca gcgagcacaa
    14041 gaaatggggg taacgaaaat ggcggtcgtg ggcttcgata cgccaacgat tgaaaagtct
    14101 ttgcaattaa gccatgacta tccaaatata tacagtatta tcggctggca tccgacagag
    14161 gctggcagtt atacgaaaga aatcgaaaat aaattacaag aacaattgac tttaccaaag
    14221 gttgtagcat taggggaaat tggcttggat tatcattgga tggaagaccc gaaagaagtc
    14281 caagataaag tatttcgcag acagattgct atcgcaaaag aaatgaacct accaatcagt
    14341 attcatacta gagaagcact tgaagacacg tatcaaatcc taaaagaaga agatgttcga
    14401 gatatcggtg gaatcatgca tagtttcagt ggtgattatg aatgggcaaa acgatttctt
    14461 gacttaggga tgcatatttc atttagcggt gtcgtcactt tcaaaaaagc attagacgtc
    14521 caagaggctg cagcaaatgt accaatcgaa cgattacttg tggaaacaga tgccccttat
    14581 ctggcaccag ttccttttag aggaaagcga aatgaaccag gctatactcg ttatacagta
    14641 gaaaagattg ctgaacttcg gaatcttccg acagaagaag tggctgccca aacatggaca
    14701 aatgcgcacc gattatttgg tctaagcgaa catggataag atgaagatcc aagagattat
    14761 agtcgttgaa ggaaaagacg atacaagaag gctgcgggaa gtagttgatg cagatacgat
    14821 agaaacaata gggtctgcaa tcaatgaaga gatcctgatg caaattgaac atgcccaaga
    14881 gactcgtgga gtgatcgttt ttaccgatcc tgatttttca ggagaaaaaa taagaaaaat
    14941 cattatggaa gctgttcctc aggcaaaaca tgcttttttg ccgcgcacac aagctaaacc
    15001 aaagaaaaag ggaagccttg gtgtcgagca tgcaagtgat gctgctattt tagaagcatt
    15061 gaaaaaggtc gtgacgccag acatttccca aacggctgtt tctgaaatca ctagacaaga
    15121 ccttttagac tatggtttaa tagctggggc acaagcaaaa gaaaaacgag aaaaactagg
    15181 tgacgagttg cgcatcggtt acacgaacgg caaacaattg gaaaaacgtt tgaagatgtt
    15241 tcgtattacg ccgaaagaac tcgcccaagc aatgaaaata gtggaggaac aacatgagtg
    15301 aatatcgtga aattgcgaca ccctctagaa caaaagagat tttattgaaa catggttttt
    15361 catttaaaaa aagtttagga caaaattttc tgactgaacc aaatatttta aggaaaatcg
    15421 tagaaactgc gcaaatagat gaacatacga acgtgatcga agttggacca ggaatcggtg
    15481 cattgacgga acaattagcc aagcatgcaa aacaagtcgt agcatttgag atcgatgatc
    15541 gtttgatccc tgtattggct gatacgatgc aaccctatga caatgtgact attgtacatc
    15601 aagatatttt aaagaccgat ttgtcaacag ctgttagaga aacatttcat gaggagcttc
    15661 cgttaaaagt cgtagcgaat ttaccttact atattacgac accgatcatg atgcattttc
    15721 ttgaatcaga tttggttgtg gatgaactag tcgtcatgat gcaaaaagaa gtagctgatc
    15781 gtatctctgc tgaaccagga acgaaagctt atggttcatt atcgattgct gttcaatatt
    15841 atatggaagc cagtttagca ttcatcgtac caaaaacagt atttgtccct cagccaaatg
    15901 tagattcagc gattttgaaa ctgacaagaa gagatatacc ggcagtcgaa gtgacagacg
    15961 aaaaagcatt tttccgattg acaaaagcgg catttcagca aagaagaaaa acgctttgga
    16021 ataacttgca gcattcgtat ggcaaagatg accagacaaa agcatggtta gcaaaaagct
    16081 tggaaacagc tggaatcgat ccaaaacgtc gtggggagac tttgtcatta caagaattcg
    16141 ccgcattaag caatgcgatg tcagaaaata tgcagtaaaa aaagaggttg tgacagaagt
    16201 tgtgaaaaag ctgtcctgca tcaagtaata agaacagcca aacaaaaata gttcctcatg
    16261 tttttcgttt gtttgggctt attaccgcag atgcaagctt ttgaacaccg tttaagaaag
    16321 aggttgtggg cctgccgttt aactctaagt cacaaggaac aatttcaaaa aacagcttct
    16381 catgtttttg agttagttgg tcttattact tgatgcaagc ttttgaccac tattatgccg
    16441 aacaaggaga tgtgacacaa attttgtcac atctcctatt tttgtttctt gatcaaaaaa
    16501 ttcttatact tgatactcac actcattttg tatggattta gaaaagaata tgtgtcttga
    16561 cagttattag taagtgagtt atactaagtc gacatttatg tgtcaagaca tattaggaga
    16621 gagaaaaaat gagaaaaaat tcagttcgaa aactaacgat ttctgcttta ttgattggga
    16681 tgggggtcat tatcccaatg gtcatgccta aaatcatgat tccaccagct tcctttactt
    16741 tggcgtctca tgtgccatta tttattgcga tgttttttac accaggagtt gcagtggctg
    16801 ttgcgctagg tacgacattt ggtttctttt taacaacgcc ggttatcatc gcattgcgag
    16861 cattgtcaca tcttgtgttt gcgttgattg gggcttggta cttgcaaaag catccgagta
    16921 tcgttttaaa aaatggacag tttacttttg ctaattggcg tttccaaggg ttcaattttg
    16981 tgatcggtgt gattcattct ttagcagaaa tgctagtagt cagcatcttt tactttattg
    17041 gtaatatgcc tgaaacatat tattctcaag gatattttta tacagtattc atcttgatgg
    17101 ggattggtgg gcttatccac agtttagttg attataatat tgcttatttt atagcaggaa
    17161 cactcagcaa atattttgat atccccgtat ttacggcagc aaaaaaagaa aatcggaaag
    17221 agtcgattca agggtacgcg aagaaaacga catattagac atgatctctc aaaaaaaaac
    17281 tctcatctag taatggataa taaatcctct aagatgagag ttttttgtcc taaaaagagc
    17341 tgcaccaaca tcttctggca cagctctaac agggggcaat ttatggaagt ttgatgactt
    17401 gttctgtttc tttatcaaag aagtggcctt tgctgatgtt gaaggcaagg tcgactattt
    17461 cgcctggttt atggaaatct cttgcatcta cttttgagat gaactcagta gaaccagttt
    17521 ttgtgtaaag catcgtttca gctccaagta attcggaaac gacaacttct gatttcacta
    17581 cggcttcagg tgaagcttca agggctactt gttcactgtg gatgtcttct ggacgaatac
    17641 cgaagataac ttcttttcct tcataacctt tttcaactaa tactttgttt tttccttcag
    17701 gaatacgaag cttaagccca tgtccatctg tgataacacc atttttcaat gtcacattaa
    17761 agaagttcat ggcaggagat ccaataaagc cggcaacgaa catattgacg ggtgtatcat
    17821 aaacttcttt cggtgtaccg atttgttgga taaatccgtc tttcatgata acgattcggt
    17881 cagccatagt cattgcttct gtctgatcgt gagtaacata gatggtcgtt gtttcaagac
    17941 gctggtgaag tttggcaatc tcagcacgca tggctacacg taatttagca tctaaatttg
    18001 ataatggctc atccattaag aagacttttg catcccgaac gattgcccgt cccaatgcca
    18061 cacgttggcg ttgtccacca gataaagcag ctggttttcg tttcaagtat tctgtcagac
    18121 ctaagatctc tgctgcattg tccacacgtt ttttgatgtc tgctttatca tatttacgta
    18181 atttcaagcc aaaagccata ttatcaaaaa cagtcatatg aggatacaat gcgtagtttt
    18241 ggaaaaccat ggcaatatca cggtctttag gtgccacgtc attcatgact tttccgccga
    18301 tatttagttc tccttcagag atatcttcca gtcccgcgat cattcgcaaa gtagttgact
    18361 ttccacaacc ggaaggacca acaaaaacga taaattcacg gtctttgatt gatagattaa
    18421 aatcagttac agaataattt tctgcattgt catatttttt atatacgttt ttcagagcca
    18481 tttctaccat aatacgttcc acactttctt ttttagtttt attaactatt taaagtataa
    18541 atgaaagcgt taactaactt caatgtcatg ttgaacaaga attttttctt ttttttgtca
    18601 atgtgctagt tttaagcttt aataaatatc cataagaaat aaaaactgat acactggagt
    18661 aaataagtat gttaacatta tcaaaaggag tgtctcttat gaaaatcgct ttgatcgctc
    18721 atgaccgaaa aaaacctatg atggtgaaat tagcaacagc gtataaaaca atattggaaa
    18781 aacatgaact ttttgctacc gggacgactg ggcagaggat cacggaagca accggcttgc
    18841 cgatccatcg gtttaaatcc ggaccgttag gcggggatca gcaaattgga gccatgattt
    18901 cggaggatca gttagatttg gtgatttttc ttcgagaccc gctggctgca cagccccatg
    18961 aaccagatgt aacagcactt atccgtttat ctgatgtcta tgagattccg ttagctacga
    19021 atatcggaac agctgaggtg ctactaagag gactggaagc cggcttccta gattttcgaa
    19081 gaatcgtcca tgaaatagac aagcatccac tttcattttg atggataagc gtatggaaaa
    19141 ataacgttta atgttcgcct atatgtagct ataaaagcta aacaaactga taatatgcga
    19201 tataatacga acgtattgga taatataaac aataatatta gtttttcgta tttgcagtaa
    19261 atgtgaaaat atgctatagt ttctgtggga aaagatccca aaaataatac agagtagaaa
    19321 ataaagcgtt aagtgctaag aggatgggat gttgcctctt ggacgaagga cgtaatgttc
    19381 gcggttttat tttcgcattc gctgcatgat acgatgtagc agctcttaaa ggagatgcta
    19441 aaaatgaaga aacaccggtt agacgcacgt atgattgcta ttatgggctt attgattgca
    19501 ttgatggtca ctttgtcccg actggtagca atcgagaccc catttattaa aatcagtgtc
    19561 acatttattc cacaagtgat catgggcatt ctttttggtc ctttttggtc agggataggc
    19621 gcagttttag ctgatttagt aggaatggcc ctcttctcga aatctgcatt ctttattgga
    19681 tttacattga atgcatttat tgaaggagca atctacggat tctttttcta tcgaaaagag
    19741 atcacttgga aaaatgccat tctggctacg cttagtgtca cgctgatcat caacttattt
    19801 ttgacaccat tatggcttgc gttgatgtac catgtgccat tgttcagctg ggtcgtttgg
    19861 gcgccccgcc tgttaaaaac agtaatatgg ttaccgattc aatcaattgc catttattac
    19921 gtaggacgta gtattcctta caaaaaaatt ttaagaagtt tagcgatcca cgctaaataa
    19981 aaaacaagca gtcttcccat cccagaagac tgcttgtttt tattgtgctg tgaattgtgc
    20041 ttgatagagc cgtcggtaat aaccatcttt ttgaagcagc tcatcatggg tgcccatttc
    20101 gacaatagct ccctgatcca tcactaatat ctggtccgca tttttgattg tggacaggcg
    20161 atgggcaatc acaaaactgg tttttccttc catcattgct aagaaggctt gttggatttt
    20221 tttctctgtc aatgtatcga ctgagctggt agcttcatct aagatcaaca tagggggatt
    20281 actgatcatc gttcgagcaa tcgtcaacag ttggcgttgt ccatctgata tctttaaccc
    20341 ttgaccgcca atcttagtat ctaatttttc cggcaaacgt tctacaaatt catacatata
    20401 agaggctttt agggcatcat tgatttctgc gtcacttgct tccggattcc caaaacgaag
    20461 attttctcgc aatgtactgt caaatagcca agtatcttgt aacaccattc caaaactttt
    20521 acgtaatgta tcacgttgga tagagcggat gtcatggtca tcgactgtaa ttgttccttt
    20581 tgttgtgtca taaaaacgca tcagcaaatt aacaagtgtg gattttccgg cccctgtttt
    20641 tcctactata gcaatggttt gacctggata agcagtaaaa ttgaaatctt cgatcaaacg
    20701 ctgcttgggg tcataagaaa aagaaacatg atcaaaagag acttttcctt tgatattttt
    20761 gggttccaag gcataggcat ctggcaaatc aggcttttct tctggctgat cgatcaaatc
    20821 aaatgctcgt tctagaccag aaaaagcggt ttgcagttgt gtcgtgattc cagaaagttc
    20881 gataaatggt ttagaaaact gacttgagta gatcgtaaag ctggaaatca caccaacagt
    20941 gattgacgaa ccactcttca atgccaatag gccgccaact aaaccaatcg ataaataagc
    21001 aaggtgatct acaaaacgag aagacgggtt tgtcaaggaa gaagaaaatt gtgcccgttg
    21061 tcctttttca tatagatcag catttagcag ttcaaattgc tgttgactag cttcttcttg
    21121 ctgaaaagct ttcacgattt tctggttacc gattcgttcg gtcacaaagc ccgagatatc
    21181 cccaataatc ctttgctgtc tatcgaaatt tgtttgtgaa gcacgagcaa cgatccaact
    21241 cacaaggaaa ataattggtg tagaacaaag aacgaccaat gtcagaagtg gacttagacg
    21301 catcataaaa ataaacgaaa gaatgatgat tgccactcca gaaaataatt gattgaaggc
    21361 cgcagaaatt gcaatggaaa tattgtccat atcatttgta aatcgactga cgatatttcc
    21421 gtgggagttt tgatcataat aacgaagtgg caatcgattc agatgagcaa atgcatcttt
    21481 tcttaatttt gcaacggata agtaagctaa ccgattgctt aacacttgga tgagccattg
    21541 actgactacg ttgattaaaa cgataccacc gaatagaata agtaaatgga acagtcgatt
    21601 gaagcggacc tgtccttctc caatcatttg atcgatactt tgaccaattt gtaacgtcat
    21661 cacaactgtt gcaatcccat tgatgattcc tagtatcaca gctagccaaa tgtcttttgc
    21721 ataggcagta aggtaaggaa tgaatcggcg gagcgtctgc aaggataatt tttttacttt
    21781 cataactatt cttcctcctg tgatgcgacg atttcctgat attctgctga ttgagcaagt
    21841 aattcttcat gggtaccctg agcagcaatc ttaccttgat ccatcactaa gatatgatgt
    21901 gcttcttgaa tagagcggac acgttgggag ataataatca ctgtactgcc aagataagtc
    21961 tgtagcgcct tacgcaaatc caagtctgtt tgataatcca acgcacttaa agagtcatct
    22021 aaaatcagta atgctggctt ggcaatcaaa gctcttgcaa tcgtcagtcg ctgtttttgt
    22081 ccgccagaga aatttttccc attttccatg acttttgtat ctaaaccatt tggaagctga
    22141 cggacaaatt cttctgcctg agcgattttc agagcttccc aacattcttt ttctgtggcg
    22201 tctttcttac cccattgaag gttttcacga atagtcccgg taaatagtac agaagtttgg
    22261 ggaacaagag caatttgctg acggagatga tccagctgcc aagaacgaac atccattccg
    22321 tccattataa ctgtaccgga actgacatca tagaaacgcg gaatcaattg tgtcaaagtc
    22381 gttttcccgc ttccggttgg accaatgatt ccaaggactg tatttcgctt cagctgaaat
    22441 gtaatatttt ggagagaaag accagaatca ggagtataac ggaagtcaac atggtcaaaa
    22501 gcgataattg cttcagaaga aaaatcggga gtagtactga cctcagcttc aagaatcgaa
    22561 ttttctgtgt ctagcacctc tttcacccga tttcctgaag cttcggcacg tgtgaagatc
    22621 acgacgaggt ttgaaacaac gatcagtgcc aaaagcattt gattcatata attgatcaaa
    22681 gcaagaactt gtccttgttg caagctacca atatttactt tgatcccgcc aactgtcagc
    22741 aaacagatga tcccgacatt catgatcaag gttgtagcag gtgataatag ggcagagata
    22801 ttggaaacac gtaaataatt gttcgctaga tcatttgtgc ttttatcgaa cttctctgtt
    22861 tctttttttg tctgcgcaaa tgcgcggatg acacgaacac cgctaagatt ctgactaacg
    22921 tgttcattga gctgatctag tttttcttgc actttttggt agagaggaac ggtttttttg
    22981 atgataaaaa ataaaatgat tgagaaaata ggcaataatg ctaagaaaaa cagacctact
    23041 tcccagtcga tcacaaaagc catgaccact gacccgatac ttaaaaacgg tgctctgatc
    23101 aataaacgaa tgaccattgc taaagctagc tgaagctgat tgatgtcgtt tgtcattcgg
    23161 gtgatcaagg tatctgttcc aaaatgattc aactcgttgt gagagaaagt attgattttt
    23221 ttgattagct gattacgtaa ctctgtacca aatccttgtg aagctactga agcatagtat
    23281 tggcaaatga tagcacaaat cagaccaatc acagacatag cgaccatcca taagcccatt
    23341 tcaatgattt ttcctcggtc attcattttt agtccattat cgatcaagga agccatcatt
    23401 aaaggcagga caagttcgaa gacagcttca agaaatttga agaacgggcc caaaatgatt
    23461 tgcttacgat agtcttttgc ataacgtaat aactgaatca tatgattcct cctattattt
    23521 atttgtccac aattgaaaaa acaagtacta ccacattgta gcttaggaaa tttagaatga
    23581 aaagagaaca agccttttca taaatgaaag ttttattatg aagaagcttt agaaattgaa
    23641 tagactattt tttttaaaaa aaagaaaatg agaaaacatg ccagtctctt cattcatagg
    23701 aacgtctatt ttacttaatt tttctataaa tgaattctgc ataaaaaata tatcaagtaa
    23761 atgtttatag atgaaactct atttacttct tttttaattt gttcttgcaa tcttttttgc
    23821 tacgtaattt ttgtaaaaaa gtgggttgag agaaatctaa ttgattttca aagaaggagt
    23881 atgaaaatga gaaaaatatt tattcgttta ttgtgggtac tatcttagag gaataaataa
    23941 tagtggagaa aactatggtt tggcgcagat tctatcaggt ttgatggaac gaagacaacc
    24001 tcccgacatc aagtcaaaag ctacttgaga ttttgatctg aaaataaaaa aagaaaacaa
    24061 ggtaggaaca tcaatgtttc taccttgttt tcgtaatcaa tgggaaaaaa ctaagagtgg
    24121 tgtacgtaat caataaaaag gatggtgaca acagtacctc tttactctct gaaagcatca
    24181 taactcgttt ttcctcatta tgccaataaa ttgttgttat accacatata aggatttgta
    24241 atagctaaaa acagacagga aagttgaaat ctgtgaatct tattttgtat ggaaggattt
    24301 atagaacgca aaaatcaaga taaagatgaa taaaaatcct gtttcggcta tatattgcgt
    24361 atctacgcca ataaaacgag tttgttctgt catagccata taaagcaaag gcaaaaatac
    24421 aactacagcg agataagatc cgatgatttc tcgaaaagtt ttccaggaag tatgtttcct
    24481 gtttttatat ttatccgatt tcattgaata gtcatagcct ccttattctt tcatatgttg
    24541 aaattccagt ataaaaactg gaaggaactg tacgtttttt agaataaaaa ttataaggga
    24601 taagtaggct gctgtctata ataagatata tttttcaatt ctttattaaa gattttattg
    24661 acaaaatgaa tccgttttct atgatggagg aggggtgaat aagttgttga agaaggaaat
    24721 tttatgttac ttagagaacc agacagcctt tttcgattta gaacatatga acgaggtctt
    24781 tacagcacaa aggttagctg atcattttgg ggtaaaaaga aacacagtca gtcattattt
    24841 gaaccagtta aatgatgagc aactactagt caaaatcaat tctcgaccag tgatttattt
    24901 ccacaaagaa gcttttgaaa aacaattttt taagttacgc acgaattttt atttttctat
    24961 cgatgatctc aaagaggaac agcctttttt cgcgcaacca aaagacttat tctcttttat
    25021 gatcggatac gacgccagtt tgaaagaaag tatcgaacaa atcaaaacag ctctttacta
    25081 tccagatggc ggattaccac ttttgattac aggcgaaagt ggcacaggta aaagttattt
    25141 agttaatttg gtctatcagt attgtctgct tcatgatctg ctagaagatt ctgcaccatt
    25201 catcactttt aattgtgcac aatatgcaga taatcctgaa cttttgacta gcaatttgtt
    25261 tggacatgtt aagggagcgt acactggtgc agaagaaaat aaaaaagggg cttttgaagc
    25321 agcagatggt gggatcttgt ttttagacga ggtacatcgc ttaacaccag aaggtcagga
    25381 aaaactgttc acctatcttg atcaaggtgt gatttatcgt atgggagaac ctaatttgtc
    25441 acgccggatc aaaacacgtc tcttttttgc tacgactgaa gagattacta gtcatttctt
    25501 aacaactttc attcgaagaa tacctatccg aatcgaacta cctgcactta atcagcgtag
    25561 tcgtaatgaa cggctagaac tgatttactc tttttttctc gaagaacaaa gaaaaatggg
    25621 gagccctttg tcagtcagtg gaccagcttt gtcactttta acaagtcaga aattatccgg
    25681 aaacatagga gagctgaaaa atatcgtcaa agtttcagca gcaaaagcat atgctgaaca
    25741 gcgtgaacag cctgaaatcc atgtcacgat ccatcatttg caaaaagaac tacttgcgca
    25801 gcctattact aaaaatacag cacagcaagg aatctatatt acgcaagacc agaccttaga
    25861 gcaattaatg gagaagcaac agcccgaaca gcagcgaatc gttaaaagtt ttgagcaaat
    25921 cctaattgat ttcaagaaaa ataactgtct gctatattct tgcgaaggaa aattaaagca
    25981 agaagtgatt caattatttg attttttgct gtttgagacc agtagacaag aaaaacacga
    26041 gctgctggtt tattggacac aatctatccg agagaccttt cgacaaatgg aaactgccta
    26101 tcaaatcaaa ttcgatggaa acagtgtcta tgccttgagt tactatctct atcagcgaag
    26161 cactatcaaa tggttaccgg aagaacatga tattatccaa ctaatcaaag aattagaaca
    26221 acaagtttct cagtcttatc catctagtta tcactatgtc caacgaattc tggaattaag
    26281 taagatcaaa ttagatattg aagacacaag tatggatcgg attttactga cgatctatct
    26341 aaaaaaagcg aaatggtcaa aagaaaaacg actgccaaaa gcgatgattg ctgctcacgg
    26401 ttatgcaact gctagcagta tggcaaatgt agtaaatcgt ttattacaag aggatctatt
    26461 tgaatcattt gatatgccac tagatgtcac accacagcag attgcttcag aaatccttga
    26521 ttattgcgaa aacagcgatg tttcaaatgg attgatcata ttagtagaca tgggatctct
    26581 gaaagaaatc catcagtttt ttaaaaaaca gttatccgta ccgcttttga ttttgaataa
    26641 tgtgacgaca cctttagcaa tcacagtagg ggaatgcctg caaaaaaatg tatcattaga
    26701 agagatagca gaagagactg ttcgacagat ccagccagaa tggcgactgc tctatccaga
    26761 ggaaaacaaa cccaaagcac tcattactac ttgcttcact ggaatcggta cggcggtcca
    26821 cttaagtgaa ctgctagaaa aaagcttacc tacggcttgt cagttgaaaa tcatcccata
    26881 tgaatatcag caattaaaag ataagaaaaa cagtgaccca ttattttcca tatacgaagt
    26941 cgtaggaatg attggcacca cagatcctga tatcaccaat atcgactatc tttcattaga
    27001 agacctcatc tcgggggaaa agatgtctgt actagctgaa tggttggaca ctactatgaa
    27061 tgcttcagag aaggaaatat tcaatcagcg catcattcgt aatttttcat tggaaaaagt
    27121 actggattcc gtgactattc tagatacaga gaaggtgatg ggagaaatcg atcattttat
    27181 gagagaatta gaggtgcagt tgaatcgaag catcagcaat cccaaaaaat tagcgttgta
    27241 tgtccatgtg agctgtctca ttgaacgatt gatccgacaa atgccaatcg aaacctatca
    27301 aggattagag aaatggaagc agtgtcaaaa agaagggtta tctgctatta aatctgcgtt
    27361 tagtgtcatt gaagagcatt atagtgtcat gatccccaat tctgagattg cctatatcta
    27421 tgatattttg agtgaagata cagagtttct ctcgatagaa gaagaatttt gaacataagc
    27481 aagttgttgc cactaatttt ggcatgcaac ttgctttttt agtatgtgta agtgaaaaga
    27541 ggagatgaaa aagtggatag agaaattatc ttagcttccc atggaaaatt tgcttcaggg
    27601 atattagatt cattagagct gatctatggg aaaaatcatt ctattactgt attcgattgc
    27661 tatactgatg agaattttga tttagcggaa acagtaaggc agctttttat gaggtacaaa
    27721 gataaagaaa tcattgttct gacggatctt tttggcggaa gtgtcaacaa cgaattctta
    27781 caatatatca atcgggatca tgtctatctc gttgccggat tgaatttgcc actattgatt
    27841 gagctagtat ctcggttgga cgctgaagta gagacaactg ttttgatcca gcaggtgcta
    27901 actgattcaa aaaaaatgat ccagttttgt aacgagcggc tcaatcagaa aatagaagaa
    27961 gacgaatttt aggaggaaga aaaatgattg tattgacaag agtcgaccat cggttattac
    28021 acggacaagt agctttttca tggacacaaa caataggcgc agactgtatt ttgattgcta
    28081 atgatgatgt acctacgaat gagatacgaa agacaacgat caaattagcc aagccacaag
    28141 gagtcaaact agtgatcaaa tcaattgatg actcgattgc cgcgttgaaa agcggggtga
    28201 cagataaata taagttattc atcgtagtgg aatcgatcga ggatgcatat aagctggcag
    28261 aagcgtatcc agaaatcaaa accatcaact taggagggat caaggcaaaa cctggtactc
    28321 gcagcatcgg aaaagcggta aatattttac cagaagagga ggaacgcttg aaacgattaa
    28381 ttgacaaaca aatagaagta gaaatccgtc aagtaccagc agataaaaaa gtggatgtaa
    28441 gagaagtttt gtaagaaagg aagaacctca atgattgtac aagcagtctt attaggaatt
    28501 gttgcatttg tcgcacaatc agaatatgca ctagggacat cgttgctttc tcgtccgatt
    28561 gtgacggggc tgtttacagg aatcgtgtta ggtgatgtaa aaactgggat cattatgggc
    28621 gcaactttag agttagcatt tatcggatcg ttctcagtag gtgcttcgat tccacctgat
    28681 gttgtgaccg gtgggatttt agggacggct tttgctatta cagctggagc cggtacagaa
    28741 accgcgcttt tactaggttt gccgattgct acgttgacat tgatattgaa aaatatttac
    28801 ttagggcttt tgattccaat tatgaaccat aaagcagaca attatgcaga agaaggaaat
    28861 tataaaggag tcgagcatat gcacttgctt gccggtatcg gtctttcctt gatgcttggt
    28921 gttgtggtaa tggtttctta tatggtggga agcaatacga tcggaaatct attaaatatg
    28981 attcctgact tcgttcaaaa ggggttgtca gtagctacag ggttgattcc agctcttggt
    29041 tttgcgatgc ttgctcgttt gctgatcaac aaacaagttg caccatattt tttcttaggt
    29101 tttgctatag cttcttactt agagattcct gtgaccggag tagctatttt tggtgcgatt
    29161 ttagcagttg tggtagtcaa tattatgaat gtccgtcaac taccacttca aacaactgca
    29221 ggggaggtag aagaagatga agaattctga gatcaaaaac agcaaaataa cgaaaaaaga
    29281 gctaaatcac gtattttggc gttcttttca aatggagttt tcttggaatt atgaacgtca
    29341 gatgaatatg gcttatgttt atgcaatgat tccgattttg aaaaagttat atcagtcgaa
    29401 agaagaaatg gcagcagctt tgaaacggca tctagagttt tttaacacga ccccacatat
    29461 cgttacactg attttaggaa tcacggctgc aatggaggaa gaaaataagg atgatcctga
    29521 atttgatgtg actgcgatcg atagtatcaa aacatctttg atgggacctc ttgcagggtt
    29581 aggagattct ttcttttggg gaactttgcg attgattgcc acaggtgtgg gtacctcttt
    29641 agccatgcaa ggaaatattt taggccctat cttgttcatt ttgattttca atattccgca
    29701 cattctgttt cgctatctag caacaggttg gggatatcgc ttaggaacgg gttttttgaa
    29761 aaagatccag gcaaacggta tgatggaaag tttgacatta ggcgcgtcta ttattggact
    29821 gatggtcgtt ggagcaatga ctgccacgat gatcaccatc aatattcctt taaaaatcgg
    29881 ttcaggagaa aatgctgtga ctgttcaaag tatttttgat gatatcgttc caaatatctt
    29941 atgtcttgga gcatttggca tagtgtttta tctattgaag aaagaagtga aaccattaac
    30001 gatcttgatc ggactagctt tctttggaat ttttggttcg tggataggga ttttttaaaa
    30061 aaggagcaga gaaaatgttt acgatgcaag attatatcta tgaagaaaag gaagtactaa
    30121 gtacgatctt gaaaaagaat gatttttcga caagagaaca tatgaaaaaa acagtgaatt
    30181 tactgatctt agcaacgggt tcatcttata acgcttgttt agcagcgaag ccagcattgg
    30241 aatcttatgg tgatcttact gtcgatattc aagaaccttt ttattttttc aattatggaa
    30301 aactttctcc ttcaatcgat actgtgattg ctgtgtcaca aagcgggaaa agtgcttcta
    30361 ctgttgaagc agtaaaaatg atacaaaaac aagggttacc tgttgttgct atcacaaatg
    30421 acgtgcaaag tccacttgct ttagaagctg cacaaattat tgatttaggt gccggggtag
    30481 aatcagtagg cttcgttaca aaaggctatt ctgcgactgt tttacagtta ctactcttag
    30541 ggataggaat tggaataagt aaaaataaaa tcagcaaaaa aatagaacaa gactacatgc
    30601 aacagttgcg caaaataatc aaccatttac cagcgattat tcaaaaaacg gaagaatttt
    30661 ttgatggata tcaaagttta tttcgtttag cacagcgttt cgtcaccatt ggatatggtc
    30721 caaactgggg aacagctaaa gaagcagaga cgaaattgac tgaaacaatc cgcgttccgt
    30781 cacaaggatt tgagttagaa gcttatatgc acggcccata cctggaggca gatgcttcgc
    30841 atttgctatt ctttattgaa ggggaaagtg taaataagga acgttcgcaa aagttgcaaa
    30901 gatatatgag tcggtacgtt ggagagactc tcaccattac cacaaaaaaa gcaagaaacg
    30961 agaaaacact tggattagca atagaatgtg atgaatatct gtctgtatta gcgcttgtcg
    31021 ttccgtttca actattcgct tataaaaccg cagtagctaa aggtattgac ttgaacaaaa
    31081 agatttttga agactttgat acagtcttga aaagtaaatt ataagaggtg gaaatcatgc
    31141 tgaaattcaa tgaagaagaa caaatcaaag caataaaagg cgcactagct ttacgtccgc
    31201 aagtagaaga aatcatcgat aaactttatg cggaaaaatt tgacgctgtc tattatctag
    31261 gaatcggtgg aacgtatgct tcctcgatgc aagctgtcac ttatatgaat gggaaaagca
    31321 acttgcctgt ctttgtacag catgctgcag aatattacac tactggaaac aaaagattaa
    31381 caaaagattc tatcgttatt ttgtcctctg ttaccggtac aactcaagaa gtagtgaaag
    31441 cagtagaaca aatcaagaaa gtaggagcga cactgattgg atttatcgat aaagcgaaca
    31501 gtaagttgtc tcagctatgc gattttgtgg tcacttatcc agctcctgga acagaacaaa
    31561 tcaaattttt tatggcagca gatcgattga tgtatttgaa tggagaattc gaagcttatt
    31621 cagaatacta tgaacagctc gaacgttatt taccaacagg gttagtggaa gcagagaaaa
    31681 aagcagatgc atttggctta tcatttgcag aaaaacatcg acgtgatagt atgcattatt
    31741 ttattggagc agggaatcaa tggggagctg tttattcata tgccatgtgt tactgggaag
    31801 aacaaagctg gcttccatcg aaatctatcc atgctgccga gtttcttcac gggacgttag
    31861 agatcgtaga agaaacaacc ccagtcacat tatttttagg cgaagatgaa cagagagtgt
    31921 tatctgaacg tgtggctaaa cttttgccaa aaatttgcag caattataca ttgatagaca
    31981 caaaagacta tccagtagaa ggtatcagtg aaaaatatcg cggacgtgtt ttgtctttct
    32041 tattgatgca tgtagtgaca caacgcatcg atgctcatgt ggagcagttg aactgccatc
    32101 cattagagat acgccgctac tatcgtcagt ttgattatta ggatagactg gataaaaaga
    32161 atataaaaaa taaagagagc agcgataact gctcccttta tttttttaaa atattggcat
    32221 caattacgtt ataaaacaca ttttttgcat tatctacagt tcaacttata atctgtttaa
    32281 tgggacatgt gggtgccaac cattttttgt tatgcaatag tactatttag ttgttgcatg
    32341 gatccgtagt aaaagtccaa gtacaacgag ttgcgccagt ttacatagcg ttttgtatcc
    32401 agaatgttga ctaaaacaaa agttcctatg tcaaccgtca acagagacaa tctgtccgtt
    32461 gcaggacctg acagatttag aaatgttcaa tctgtctctt atgtatttta aatcaatact
    32521 cactaggaga agtttcctaa cctcctaata gtaatgctgg tagtaacatt gtcaaaactg
    32581 cttcctctta ggaacaagca aagcataacg agtgtaaaaa taaaatgtag actattgtaa
    32641 catatcattg attatttcaa gtaaaaacga taaaagaagt aaacaaacac ttaaaaacaa
    32701 tggaaattag tcccaacttt aagaaatgta tactataatg gaaattatca attatttatt
    32761 tggaggaata tccatgaaac aaaaaatcgc agttcttggt cctggatctt ggggcactgc
    32821 tttagcacaa acattggcag aaaacgggca tgatgtccgt atttggggaa atgtacctga
    32881 acagattgat gaaatcaata agtatcatac aaatcagcat tttttacctg atttgacgat
    32941 cccagaatcg attatcggat ataaagaatt ggcagaagca gtggataacg cagatgctat
    33001 tttatttgtt gttccaacaa aagcgattcg ttcagtcgca caggaactta ttggtaaaat
    33061 gaatactaag ccaatcatta tacatgcaag taaaggacta gaacaagata cgcataaacg
    33121 aatctcagaa gttttggctg aagaaattcc agaagaaaaa agacaggcaa ttgttgtatt
    33181 atctggtcct agccatgctg aggaagtggc agtacatgat atcacaacaa ttacagctgc
    33241 aagtattgat caaaaagcag cagcctatgt acaaaaactg ttcatgaatg attatttcag
    33301 gatttataca aataatgatg tgattggcgt agaaactggt gcagcactga aaaatatcat
    33361 tgcaattggt gccggcgcta tccatggatt aggattcgga gatgatgcaa aagcagcaat
    33421 catgactcga ggattggcag aaatcagccg tcttggtgtc gcgatgggtg ccaatcctct
    33481 gacatttatc ggtttgagtg gtgtggggga cttgatcgtt acttgtacaa gtgttcattc
    33541 tcgcaattgg cgtgccggta acttgttagg acagggacat aagctggaag aagttctaga
    33601 aaacatggga atggtcgttg agggtgtggc aacgacaaaa gcagcagtag agctagctca
    33661 acagctaggt gttgagatgc caattactca aacaatctat aatgttttgt ataatggaga
    33721 agatatcaaa aaagcagcaa aagaaatcat gttacgtgac ggaaaaatgg agaatgaatt
    33781 ttctcttcgt taaatagatg atcaatagtt aatcgtcgtt taggaggaac agcaagtgaa
    33841 ggtcaaaaaa gcagtcattc ctgccgctgg gctaggaaca cgttttttgc cggcaacaaa
    33901 agccatggca aaagaaatgc ttccaatcgt ggataaacca acgatccagt ttattgtgga
    33961 agaagcattg gattcgggaa tcgaagacat cttgatcgta acgggaaaag aaaaaagacc
    34021 cattgaagat cactttgatg caaatatcga attggaaaac aatttgcggg aaaaggaaaa
    34081 aacagaatta ctcgctttag ttgaagaaac gactcaagtc aatcttcact tcatccgtca
    34141 atctcatcca agaggactgg gggatgccgt gcttcaagca aaagcattta taggaaatga
    34201 accgtttgtc gtcatgctgg gggatgattt gatgaaagat gatatcccat tgactcaaca
    34261 attgatgatg gattatgaag aaacacaagc ttccgctgtt gccgtcatgc gtgtaccaga
    34321 aaatgaaaca tctaaatacg gaattattga tcctaaaaca gatagcgaaa agggtcgttg
    34381 cagagtaaaa gggtttgtag aaaaacctga aatgggaaaa gcaccaagta atttagccat
    34441 tatcggacgt tatctactga ctcctaaaat atttgagata ttagaaactc aagaaccagg
    34501 agcaggaaac gagatacagc taacggatgc acttcagact cttaaccaga cagaggcggt
    34561 ctatgcgaga gagtttaaag gaaaaagata cgacgtaggc gataagttag gatatatgaa
    34621 gacaaatatt gaatatggac ttcaacattc ggaaattggg acttcattaa gtgcatatat
    34681 tatccagttg agtaaaaagc taagaacaga aagttaactg gaaataagtg gcctgctgag
    34741 aatgaaaggc tatttgttat taaagcggca gcttgtgcgt catttgcgca actgtcgttt
    34801 tagttttttg actctagatc agttgagtcg tggaaaacga aagatagaaa aagactttcg
    34861 tgaatagatt cagaaaatat tatgctaatg gtttaatttt tttttaagtt gtataaaaga
    34921 aaatgattac ataagagaaa tgaaagccaa gccagaagat tcatagtaga ataaaaaggc
    34981 gaatttttta attataaaaa aaggtgtgtg ttcactatgg gaaaattagt cgctgttgca
    35041 cagtcgctgc ttgatatact caagtggctg atggaaggaa gcaattggat atttgtgttg
    35101 ccactattgc ttattagtct atttatcatg tttcgcatgg tcagaaaaaa agaaacgttt
    35161 ctgattttta tctggtatgc agcatccgtt ggggttgcta ccttgatggt tgaattggcg
    35221 ttaggtagtt gggaagcttg cgtatttgct ttattttttg ctgtactgta tctaatcatt
    35281 tatatcccat atatgaaaag aaagtctcgg ataggcagaa aaggtagaca tacaactact
    35341 ttttctagta cagattctct cgaacaaagt agaagaacga gaagaagacg gtaatattta
    35401 gtaggtatac gcaaaaaaag ataaaataat ggataaacta ttgagatgag agatttattt
    35461 aattggtgga aaaaagcttt tttcacttat ataaaagaat cttttttaaa ggaatgcttg
    35521 ttgtatcggg ataagccatt ccttttttta tgttatttat atatatataa atcataccaa
    35581 tgtttttttg tattttcttg cttgcacttg gataagagat tacttacaat aaattcagat
    35641 agaaacagct gaataaaaaa aggctccctg acccttagtt ggcgctaaga atcagggaat
    35701 gaccagtcac acacatacac cctgaccgat cattttgcca tagtcaatgc agtgcatcga
    35761 cctaatttat tgtacctaat ctagggtgaa aattctaccc ctatgtagat attttccatg
    35821 gtctgcacgt tgttttattt gctatgtcaa ggtattgggc gtattcgttc aatgcctttt
    35881 ttgtgtttga aacagcaaag accacatgtg ttactgtcta actactgttt ttttatgtac
    35941 ttccttagat taggcagctg tttctattca tgtcaagtat agctaggcgg gagagataaa
    36001 tgagtttatg ttattttgac tattatcacc aattggaaga gaaattaagg atagaagagc
    36061 cgctgatgct tcaagctggc acttggatcg cgaaacaaat taatcaaggt ggtagattgc
    36121 agatatatgc tagccgcaca ctaagtggtg tagcatttga attttgggat caaaccccgg
    36181 atcttgtacc aagtatattg attgaaaatc cagccggagg tgtctatgaa acgctagaag
    36241 gtgcaggaca ggcaattatt gatcagcttt ctgtcaaatc agaagacatt tttcttcttc
    36301 tttcgaatga aggaagaaac ccggcaatca tcgaactagc tcagtggatc aaagaaaatg
    36361 gtcatccatt aatcattgtt actggatttg atttgtcacg aagtatcaaa tcgcaacata
    36421 gcagtggttt gcgattattt gaatttgctg atttggtttt ggacaaccac gccaatttac
    36481 atgatgcagt cttgactttg ccaaatctag atccagcgat ctgcggaaca gcctctcttg
    36541 caacgatctt tttgctgcag caagttttgt attttacagc aaaacagcta gtccaggaca
    36601 caagtggaaa atagttatcc acatgttgac aaagttcctt aaaaaaataa tgttccacat
    36661 aaaaatggag cgtttgaaaa aaagaaaacg attattaatg aggattaaca acgactactt
    36721 tttttgtttt agttatttta agatggatca tctttgattt taagcttgtt ttgtttaaag
    36781 tttgttattt ttataagttg tttcacagtt atgagaaatg agttataaga atatgtagtg
    36841 tcagaaaaat aaaaagtagt ggaaaaagag acaagaaaga atccatatgg attctttctt
    36901 gtctcttaat acacgttgat gaaagagtct tccccaaaac tctttacaaa gtaattatag
    36961 tacatttgtt atcattattg caatgaagtt tacctgatat aacaaaattg tccaacagga
    37021 tacctttttc taatagaaat aaaatacaca aaaattgaag gatagataat ggatatttat
    37081 aagtgtttat tatttttttt gcaatgaaat tcaatttttc aatgattgta aaatgaaaaa
    37141 aacaggacga gattttctct aatctaaaaa taaaaaaaga attttaagga aaaacagaga
    37201 aataaaatac ttaatataaa tggaaagaga aaaaaataat catacatagg agaaaaaaaa
    37261 caaaatagaa aaaagtgatt ttctaataaa ttattcatgt ggaaatgtca tttttactgt
    37321 ataattcatt tgattatcca ttttttattc atcaaaatta ctgaaaaaag aaaggaagaa
    37381 ataaatttga agaaaacaaa ttcttcagga aaaccgaaac gcgacttcac taaattatcg
    37441 acaccccata cgtatgtcat tatttttggt gtagtcatct ttgcatggat actgactttt
    37501 gtcgttccag caggaaagtt cagcacgcaa gatattgaat acaaagatgc aaatggcgaa
    37561 acaagtactc gtactgtttt acgtcaagat tccttccgtt atgcttacga gctggataag
    37621 tcgtatgtgt ttgatcaatt agaagaactg caagatcatc cagcagaacg tgaaaaactg
    37681 gatgttccgg aaaaaggatt ggaaaaagtc attgctgacg gagaaaaaaa tcttacgcag
    37741 gaaaaactag atgaaatctc cttgacagat gatgtgcttt acgatcagta cggcgaaaat
    37801 atctatgata cttctaaaaa attgcataaa acagccaaaa tatggggaac agatgatttt
    37861 ggcggctttg gtttcttaaa ttttgttttt gaaggactag tatctggaga taagtatgga
    37921 tctgctgtag ggatcgctgc attgatcctt gttgttggag gtgcttttgg tattattatg
    37981 cgtaccggag cgatcgacgc aggtatctat gcgtttatca gtaaaactaa gggactggag
    38041 cgtttagctt taccgctatt attctttgct ttttcattcg gtggcgctac attcgggatg
    38101 gcagaagagg taattccatt ctcaatggtg atggttccat ttgtcattgc gttaggatat
    38161 gactcgatcg tagcagtaac cgtgacttat gtcgcatctc aagtcggaaa tgcgacctct
    38221 tggatgagcc cgtttagcgt agcggtggct caagggattg ctggaatccc cgtgttgtct
    38281 ggtgcgacat tccgattgat tatgtgggta gttgttactg cattagccgc tggttatatg
    38341 atgatatatg cagaaaaaat ccgtaaaaaa ccagaaagtt cgttgactta caaatcagat
    38401 gattatttcc gttctcatat caaaaagaca tctgatgaaa ataaaaaatt catgcttggg
    38461 cataaattga tcttattaga gatgttagtt gtccttgtct ggatcgtttg gggcgtgact
    38521 caaaaaggct attacattcc agaaatcgct tctcaattct ttgtgatggg attagttgct
    38581 ggaatcattg ctgttctttt caaattagat ggaatgaaag tcaatgacat cgcttcttct
    38641 ttccaatctg gtgcagctga tttagctggt acagcaatcg tagtaggtat ggccaaaggg
    38701 attcttcttg tcttgggagg atctgatgca tctgttcctt cagctttgaa tacgattttg
    38761 catgggatcg ggacggcgtt gaccggtgtt ccagctgtca ttggtgcttg ggcgatgtac
    38821 atcttccaaa gtttgttcaa cttggttgtt acatcaaatt caggacaagc agctttgact
    38881 atgccaatca tggctccact ggcagattta gtaggtgtat ccagacaagt agcagttctt
    38941 gcttatcagc ttggttcagg ttttatggac gcttttacac ccgtatcagc cagcttgatt
    39001 ggtgtgctag gtgttgcacg gatcgaatgg gcaaaatggg caagattcca aatcaagatg
    39061 caaggattcc tgtttgtttt aggaactatt ttcatcatga ttgccattgc aataggtctg
    39121 cagtagtact ggagacctgt aaataaaaaa gtaatccaaa cagtagataa ggactccaag
    39181 aaccgtcatt ttacggtaaa atcaaaggag taaagatcct atcgtttgga ttatttttta
    39241 agatcctatc atcagttata cttaaagaaa gaggaaaaat gtatgaaaat cattcgacaa
    39301 attgaagtgt ttgcaccaga ttacttagga aaaatggatg tactgattgc cggtgggaag
    39361 attttggctg tagaagaaca gctagaaggc ggctacgaag gagtggaagt agaagaactt
    39421 tcaggtgagg gaaaagtact aaccccgggt ttcattgact gtcatttcca tattttaggt
    39481 ggaggaggag aaggcggtta tcaaaatcga acgccagaag tcacattgag ccaattgact
    39541 acagctggtg taacgacagt tgtcggttgt ttgggaacag acggagaagg tagagatatg
    39601 actgcgttga tcagcaaagc gaaaggtctg gaagcagaag gaatctccac ttatgtatac
    39661 gaaggttcgt accgattgcc ggtaaaaaca gtaacagatt ctatcatcaa agactttttg
    39721 acaattgata aaattatcgg aatcggagaa atcgcagtat ccgatcaccg ttcttctcag
    39781 ccaagttttg aagagttcgc tcgtgcagta gcagatgctc gtgtaggagg catgttatcc
    39841 ggaaaagctg ggatcatcaa tgtccactta ggcggaggaa aaagaaagct agagctattg
    39901 acacgaatcg tagaagaaac agaaattccg atcacacaat ttttgcctac tcatgcaaat
    39961 cgcacaccag aattatttga agcttgcgtg gcatttgcca aacgtggtgg cacgattgac
    40021 ttcactgcta gcgaagatcc tgatttttgg gagaagacag atggtgaagt tcgcttcagc
    40081 aaagcactga aacgattgat cgaggaagat gtttctttag ataatttcac gatgacctca
    40141 gatggacaag gaagcttgcc ttattttgat gaaaacaatc atttcctagg gttaggagta
    40201 ggaagtgcca aggcattgct agtagggatc aaagaagctg tgcaaaaaga aagtatccct
    40261 ttggaaatcg ccttacgagc tataaccagt aatccagcac gtatattgaa attggataaa
    40321 aaaggaaaaa tcgaaattgg tgctgatgca gatctgtgta tcttggataa agaaacgctt
    40381 gatattgata cagttatcgc taaaggagaa ataatggtcc aagaaaaaga agtcaaagta
    40441 tgggggactt tcgaaaaaag tttttaatca acagctctaa ctatcttttt tagaaaacga
    40501 gcgttctgtt tgtctgtatc tgttgttgat gttaccctga aggtagatca ataaacaaac
    40561 taagaggagg aagcacaatg gcagatttaa aaggacgttc taaagatatg aaagataaag
    40621 tagtcggtga aggaaaagaa gcatacggca aagcaacaga cgacaagagc aaagaaacag
    40681 aagggaaagc acaatcacta ggtgctgaca tcaaagaaaa agctcgaaat ttaggcgacg
    40741 aagtcaaaga caagttttct cgaaaagacg aaaaaaaata aaaaccactt attactaatt
    40801 gaatcaagca aataaaaaat ccgaaccata tctgcgagac agatatggtt cggatttttt
    40861 atttgatcat tatcctgttt tttaaacttc gattttttga aattttttat tttttagttc
    40921 aaagacttca tcacagcttt ttgctacttc aatctcatga gtcacgatga taatacagcg
    40981 attttgttca tgggcgattt cttggaaaag tttgacgata tcttgtgaag ttgtttcatc
    41041 tagatttcca gtaggctcat ccgctacgat tagttgatgt tcacagcaga tagcacgaac
    41101 gatagcgact cgctgctgct gacctcccga aagtttggtc acctgttttt ttgctaatat
    41161 ttcatcaatt cctacacgag ccagtcgatc taaagcaact tgtcgataat tctcttgctt
    41221 ggtcttagtg atttccattg ctgctatgac gttgtctaga gccgataaat aaggaagcaa
    41281 gttgtaagct tggaaaataa tcgagacatc atttttacga taacttctta acccgatttt
    41341 gttcagtgaa cgatcttgat agtatacctt tccttctttt ggtgcatcta gtccagaaat
    41401 aagcgaaaga aaagtcgttt ttccagaacc acttgctcct aaaatagcgt acatttttcc
    41461 agcttcgaaa ttcagttcta catctttata aagcgcatct tgcggatctt gataccaaaa
    41521 tcccaattgt tctgtccgta acatcaaatt cctcctttag tttaatagga tttttcttgg
    41581 gtttaatctt aatatcccta tagaagctaa aaagacagaa aacagactga ttccaaatgc
    41641 tactccggct aatagtccca attgtgcagg tttgacattg atttccaatt cgctgacttg
    41701 atcagaaaca gcaaatggat tattcgtggt tccttgttga ccatcgcgtt gaccgccttg
    41761 ttcttgtctg ttacctggca tttgtccacc gccaggagct tgctggtttt gtgtcgtggt
    41821 ttctgtctgt tggtcaagta gctgctgtcc cacggcattc gcgacgacgt ttccgctaag
    41881 cgaagcaact cctaatgcaa gaatcataca aatagccact tcggtaaaca attggagaat
    41941 gattttactt ctggattctc ctaaagatag caagacgcct aactcatgcc gacgctcccg
    42001 aatggtaatc ataatgatca gagtaagtat gataataccg gctacagcca ctagtaaaac
    42061 aacattttta gaaaaacttg ccacgttgtt taaaggttcc aacattgact gatacatgga
    42121 atcatttgat tgtaagctaa atgtatctgt gtcgatcagc ttctcagctt ctttgagaaa
    42181 ctcatccatt ttatctggat cagatagagt gtaaatagca gaatctatcg tgttatcgtc
    42241 agatgtccca tttagttcat ttgcaaaagt gtaagaagtg taaagtgtat tcgacggatt
    42301 catgaagttg aatttcatac ccatactgcc gcttgtttcg ctggattcat aaattccttt
    42361 gatcgtcatt tcataagtgg tatcttcatc ttcagtactc gtgattgtga aagtatctcc
    42421 gactgccagg tcatttgttt ctgctagagt agaatcaatg actacattgt ttgtgccttt
    42481 gtcgctgtca tctattcctt caccatccgt gatttttgct gttccatcag aaaaattact
    42541 attttgtgca gttgctgaca caccactgat ttgaaagtct gcttgagtca tttgcggatt
    42601 cccgcccatt cctccagcag gttcaccgcc tgcatcagta gtttctgaac tgtcttcact
    42661 tgaatcagaa ctagtgatgg cagttattcc atcttttgcc atagctgagg cggaactttc
    42721 aaaggaataa ctctttacat tatctaattc agctattttt tccgcgtccg ttaaggaaac
    42781 tggtgtaaga gaaaaactgc ctggatctgg acgttcttca gtggtagaac cgcttgaaga
    42841 agtttgttcc tgttttttaa atgaagcttc tcgattggca cttaaagtca ctgttgctcc
    42901 aacttctttt tgtgcttgat tcgctgcttc ttctgcagcg cttcgaatcg tgaggccagc
    42961 tagcacaaaa ataagaatgg ctgtgaacac ggcaaacaat aacaatgaac gtccccattt
    43021 tacttttgta cttttccatg cacgtttcat aaaattcata ttttgcatcc ctcctagtag
    43081 atgcatttga taatagacaa cctatcttaa agctagctta aatcttatgc tgtttgcaca
    43141 ccatcaacat gaggttctgt atatataaaa agtcactata gttattgcaa aaaatgtggt
    43201 aaaatgaaaa aagtatgttt caaaccgacg aagaagaaat gaaagaggaa tagcaaaaga
    43261 aatcctagat gctacagagg gatttttacg tgcatagttg ttctaacaat tgctgatact
    43321 agcgatttag tttatttgtt gtataataat ggggaacagc tgaatactta tataagtatt
    43381 ctggctatag cgtttcgcaa aagatccatt ttgttaatga agtatatcca aaataaaatg
    43441 cccatccaac tgtcagaacg cggatgtgct ggtaattttc ctgctgattc tccataagtt
    43501 gagaaatagt gattttgcat atgcttcatt ggaatggctc atatatttag ataaagaaga
    43561 aaatcgacat ctttaaactt ggatgtcata agaagaggtg aagtttgtga gtaccattaa
    43621 aattatccca ttaggcggcg ttcgtgaaaa tggaaaaaac atgtatgtcg cagaagtggg
    43681 cgaggatatt tttattttag attgtgggct aaaatatcct gaaaatgagt tgttggggat
    43741 cgacgtcgta atccctgatt ttacgtattt agaagaaaac attgatcgtg tagctggtgt
    43801 ctttttaaca catggacatg cagatgcgat cggggcactt ccttacttgt tgtcaaaaat
    43861 ttctgtacca gtattcggga cagagttgac gatcgagtta gcaaaattga atgtcagtcg
    43921 taatgaagca gccaaaggat ttaaggattt ccatgtggta gatgagcata cagaaataga
    43981 ctttggtgaa acagtagtga gctttttccg taccactcat acgataccag attcagtggg
    44041 gatcaatctg aaaacaaaag aaggtagtat cgtttatacg ggagatttta aatttgacca
    44101 aacggctatc ccaatgtatc aaacagatta tgcacgattg gcggaaatcg gcaaagaagg
    44161 agtcttagcc ttactaagtg aatcggcaaa tgcagaaaat cctcagcctg tagcttctga
    44221 agcccaaata gcagatgaag tatttgatac gatcaaatat tgggaagggc gtatcatcgt
    44281 cgcttgtgta gcaagtaatt tgcaacgagt gcaacaagta ttggatgcag cagaaaaagc
    44341 acaacgaaaa gttgtcttga ctggtcaaga tttcgaacga attattcgca cagcgatgcg
    44401 tttagaaaaa cttcaattac caagtgaaga tctgcttgtt acgttgaaag acatgaaaaa
    44461 ctatgatcca gaagaactga tcattttaga aactggccga atgggtgaac ccatcaagtc
    44521 tctgcaaaaa atggcaaacg gtacacaccg taatgtcaag attgaagaag gagatttggt
    44581 ttatatcact actacgccaa gcatcgcgat ggaaaccatc gtggcgaaaa cggaagatat
    44641 catttatcga gctggtggaa cagtgaagtt gatctctgag aatatgcgcg tttctggcca
    44701 tgccaatcct aatgacttgc aattaatgat caatttgatg aaaccaacat attttgtgcc
    44761 agttcaagga gagtatcgtg aattagctgc tcatgcagat ttggctcatg cagtagggat
    44821 gccatacaaa aatatctata ttactggacg cggagacgtg ctggaatata agaacaagcg
    44881 tatgagtgtt gcaggaacta caagtgcaga aaatgtaatg attgatggat taggcatagg
    44941 tgatatcgga aacattgttt tacgtgatcg aaaaatcttg tcagaagacg ggattttcgt
    45001 agcggttgtc actatcagtc ggcgagaaaa gaaaatcatt tcaaaacctc aaattacttc
    45061 acggggattc gtttacgtaa aagctagccg tgatctgatg aaagaaagca gcaatatgat
    45121 tgaaacaatc gttgagaaac atttagaaag caatgatttt gagtggagca aattaaagca
    45181 agacatccga gatcaattag gccgttactt atttgaacaa acaaaacgcc gcccagtgat
    45241 cttgccagtc attatggaag ctactcaacg tcgaaataaa aaataaacaa aaagagtaag
    45301 atgaacctct ctatagagtg ctcgtcttgc tctttttttg cgaaaaacaa ataatttttg
    45361 aatacagcat ccctcgcttt ataataaacg atagaataaa gggagtgaat caatgatgag
    45421 tcaaacaaaa gtcgaacgtt atttagaaga acaaaagatc agctatcaac cacttgattt
    45481 tagtcatgtg gtagaagaat ctttcacaga agaactacaa aaaagaggga ttgatccagc
    45541 tttgatatgt aaaactctcg tagtgaaagg aaacaaaact ggggtgatca tcgcattgat
    45601 tcctttgaac gatcacttag attacaaaaa aacgagacaa gtgacgaaag atcgtaaagt
    45661 tggttttcct ggaatggatt ttgtattaat gcatacagga tatccccacg gagcaaacac
    45721 ccctattgga ataaaactcg cacatcctga ttattgtttt ttagcagaca gttctttaaa
    45781 agaaaaagaa gaagtaattg tgtcaagcgg tgaaatcggg agaagccttc ggataaaagt
    45841 agccgattta gaaaagatcg tccatcctat ttacgctgat ttaattagtt aagggacgag
    45901 aagagaagct aagcagagca gagacctcaa acttgagatt atatctgctt ggtacttggt
    45961 gcatcctgta ttatctgtta caattgcttt gtacatactg tactatcaat ccaatgaaaa
    46021 tatatttagc agctatattt tttcattgat gtgcaagtga tgaaatcgtt tttaataaaa
    46081 aagatacaga gaaaacgcga ggtgtaaaat atgaaaatta cgtatcatgg acattcctgt
    46141 ttaacaattg aaatggaaaa tggccaacgc gtgatgattg acccgtttat tacaggaaat
    46201 ccattgacag acttaactgc agaagaagta aagacagatt ggttgctgat tactcatggt
    46261 catagtgacc acataggaga tatgattcct attgcaaaag aaaatcaagc cacaattatc
    46321 agtattgttg agattgctga ttatgcgaac agcagaggag tagattcatt cggtatgaat
    46381 atcggtggaa aacatgcgtt tccattcggc gtggtaaaat tcgtacatgc acagcatagt
    46441 tcaagctatg aagtagacgg tatcgttcag tatatgggcg agcctagcgg aattattatt
    46501 caagcagaag gaaaaacgat ttatcatgca ggggatactg cttatttcag tgatctagga
    46561 ttgcttgcag aagaattcga tattgatgtc gctttcttgc cgattggcga taactatacg
    46621 atggggccag aagatgcagc aaaggctgcg caaacaattc gtgccaagaa agtagttcca
    46681 atccattaca atactttccc tgtcattgaa caagatcccg aatcttttgt agagttactg
    46741 gaagatagtg aaggaaaaat aatgagcgtc tcggaaacgc tggaagtgta ggcagaaaat
    46801 ggcgacaaaa catgatttga ttttagaata tattgaaagt ttacctgttg gcaatcgaat
    46861 ttctgtaaga agcatcgcaa aagaattgaa tgtcagtgaa ggaacagcgt accgtgcaat
    46921 caaagatgca gaaaatgtcg gactagtttc aacaattcaa cgagtaggga ccatccgtat
    46981 cgaacgcaaa ttaaaaaaac atattgaacg tttgacattt ggcgaggtcg tcaggatcat
    47041 tgaaggggac gttttaggcg gggcggcagg attagacaag gtcttgaata aattcgtcat
    47101 cggcgcgatg actaaacatg ccatggaacg ctatattacg ccaggatcac taatgatcgt
    47161 cggcaaccgg gcggaagtcc aaaaattagc attggaagat ggcgccgcag tattgatcac
    47221 aggaggattc aatacgactg ctgagattgc aaagctagct gatgatcttg aaatgccgat
    47281 cttacgcacc tcttatgata catttacagt cgcaaccatg atcaaccgag cgttaagcga
    47341 ccaattgatc aaaaaagata tcatgcttgt cagtgatatc tatatgccag ctgaaaagac
    47401 tcattatttg cagcagacag atacgatttc tgattatcag cgtttgtcag aagaaacgca
    47461 gcattcgcgt tttccagtcg tcaaccgcca tcatcgtttg atggggattg ttacggcaaa
    47521 agatgtttta gggaaatcgc caaaccagat catcgatcgg gtgatgacga aagaaccaat
    47581 cagtgtaaag aaaacaatga gtattgcctc tgttagccat caaatgatct gggatgggct
    47641 agaagtcatg ccggtcgttt ccgatgactt gacattggaa ggcttgatta cccggcaaga
    47701 tgtaatgaaa gcgatgcagc ttgtccagcg acaaccgcag attgcagata cgatttcgga
    47761 tcagatatct ggaaatatcc atacgattga tactgaccgt gaaggaaatc gtttggaaaa
    47821 ccctaagttc aaattcgtag tagcaccaca aatggtcaat ggcgttggaa ccatttcttt
    47881 tggcgtactt agcgaaatca tttctgatgt cgcacaaaaa acaatggtga tgaaccaaaa
    47941 acgaaatatc ttgattgaac aagtgaacct acattatttg cggctgatcc aattggaaag
    48001 cgaattagac attcgtccac gtgtattgga aatcggcaga cgttccgcta aactggatat
    48061 cgaagtgttt attgaaaaca ccatcgtttc caaagcaatc gttgtttgtc aagtaatgga
    48121 acgttcttag aaagcaggag tgaaatgaca gtacagcaag aaatagtaga tacaataaaa
    48181 aaatacgata aaatcattat ccacaggcat ttgcgccctg atcctgatgc attaggatca
    48241 caagtaggat tagccgaact attacgggaa agtttcccag aaaaagaaat acgtcaagta
    48301 ggtgaaacga ttgaagggct tcgcttttta gcagaaatgc aagaagttga agatgtcttc
    48361 taccaaggag cgctagtcat cgtgactgat acagctaatt cacccagaat cagtgatcaa
    48421 agatatcagt tggcagaaaa actgatcaaa atcgaccatc atcccaatga tgacccttat
    48481 ggagatcttg tgtgggtaaa tacaaaagca agtagctgta gtgagatgat tgcagaattt
    48541 gctttgatgt ttcctgaatc tttaaagatg aacgttaagg ctgcacggtt actttatgca
    48601 ggaatcgtcg gagatacagg gcgctttttg tatccctcca cgacgtcccg tacattagag
    48661 attgcttcga tattgagaaa ttatccattt gatgcaagtg ctttgaacag agagattgaa
    48721 caaatgccga tgaaagtcgc taaactttcc ggttatcttt atcaaaatat ccaggtggat
    48781 gaaaatggtg caggaagaat aatccttccg caagaaattc ttgataaata tggaatcgat
    48841 gattcagaga cagcttcgat cgtttcactt cctggagtga ttgacgaagt gctggcttgg
    48901 gggatttttg ttcagcagcc ttcagggtat taccgcgtac gtttacgttc taaggggccg
    48961 atcatcaatg aattggctaa acgccatcat ggaggaggcc atccattagc aagtggtgcg
    49021 aatgcaaaag atttagcaga agtagaggaa atctataaag agatccaaga tatatgccaa
    49081 tcgtataaga gcagaaaaac agaatgatac ttcgttcgaa aacgtgcctt ttgtagtaaa
    49141 aatcacaaat gtatcttttc aagatcttct ttttccgtta caatagtgct cactaaagat
    49201 tttaaaggag aaagaagatg acattaccaa attgcccaga atgtggctca gagtacacat
    49261 atgaagatcg aggcttgttg atttgcccag aatgcgggaa cgaatggaac aaagaagaac
    49321 aagaaactgt catagaagac ggattagtgg tattagatgc aaatggaaac cgtctgttag
    49381 agggcgatag cgtgacagtg attaaagact tgaaagtaaa aggtgcgtca ggtgccatta
    49441 aacaaggaac aaaagtcaag aacatccgtt tagtagaagg cgatcataat atcgattgca
    49501 aggtagaagg atttggaccg atgaaactga aatcggaatt tgtgaaaaag aattaaagaa
    49561 tattcaaggt cttttcacat tcttttccta ctactaatga taaaataaag taaacgaaaa
    49621 ggagtgaaac gaaaatgtat ggttatggaa tgtatggttt tttagatccg acatttcttt
    49681 tggtcattct tggattagtt atttccggaa ttgcgtcagc gtacgtcaat agtactttcc
    49741 gaaaatacga tcaagtgaga agcaaaaaca atgtgactgg tacacaagca gcacaatata
    49801 tattacaaag tcaacagata aataatgtcg gtgttcaaca aattgccgga gatctcacag
    49861 ataactacaa ttcaggtaat aagatgttga gtttgtctca agcaactgca cagtctactt
    49921 cagttgctgc gataggtgta gcagctcatg aatgcggaca tgcggtacaa gatgcttcgg
    49981 gttatgtccc attgaagatt cgtgcagcac ttgttccagc agctaacttt ggttcgatga
    50041 tttcgttccc cttgattatg gtaggtgtat tgcttagctg gaactctacg ttaatcaata
    50101 tcgggatttt cgctttttct ttagcattac ttttccaatt ggtgacatta ccagtcgaat
    50161 ttaacgcgtc tcggcgagca atccaaattt tgagtgaagg cggactactg acggaagaag
    50221 aagtaccgat ggcaagacac gtattatttg cagcagcact gacttatgtt gcagcagcgc
    50281 tttcgacatt cctgcagctg ttgcgcttga tcttattgtt cggcggcaac cgaagagatt
    50341 aaaataaaga agttgtgaaa tcagcgcttt gatctgagct aataccgaga gttagctgat
    50401 tgaataccat ttatttggaa taagaggatg tgacaagaat tttgtcacat cctctttgtt
    50461 ttgttctcat aacctttttt catatacagt tcatgaaccg cttttattaa cttgccaaat
    50521 aacactgtat tgtgcgttgt atccagatag aaagtctccg tttcatctga aattttctca
    50581 tgctgatctg taatgatcaa atcagcatgt tctgaatatg cggtaatttt aacagcatct
    50641 ttgcgaaata actgctgcaa atggctttga agatgataat ttcctgtaaa ctgtttggtt
    50701 atctgcaaat ggatgagtaa ctgctctttc gtttctgttt gtagtaagtg ttggatcaat
    50761 tgactaaggt attgactgat ttggtttctt tccccatttt ttgaaaaaat tgtatcgatt
    50821 ttttctctta atttcttttc ctgtataaaa gaactagaat gattggacgt ggcttctgga
    50881 aagcaaagtg tagaaaaact actgctggat tcacccaaca gttcgaaaaa aacaactgcc
    50941 aacacaagaa aataaaaata aattggtatt ttattttcta aatggttgag aggaaactgt
    51001 tgagttaagt aagtcatcaa ttgaccgact tgtcgacaaa taaggttttt gcttgtggaa
    51061 aataaaaaac caatctcctg tttttgtcga aaagagattt gatttggaaa aaaacaatag
    51121 aaaaaataat taaagtaggc tctctctgtt tctgtctctt cacaagggcg actcactgaa
    51181 aaagaagtaa agtattctga ataatcatgt gtatgctgca ataagtccat caattcagtg
    51241 atttcagaag aagtttttgg aaaatatgcc tgttgtttgt ttcgtaatat gaataaagtt
    51301 aacagcaagt agatattttt ttcttgtttt gaaatttttt ctatttgctc atctgtactg
    51361 ttcattttta gtgcatttaa ttcaagtgag gaaaatggcc aatcgttacc tccaaatgta
    51421 tctgaaagaa aaaagtaaag atagagacgg atagataact catttccttc aagagtacag
    51481 caatttttta cagataggat ccgtaaatgg aattttttta aggaatgatt gagctgtttt
    51541 gtcagttttt cgagatagga gggcgatata taaagttgtt caagtaaata tttttttggt
    51601 acagcagttt gtggataatt gattagtaac atcaaatagt taaaacgatt ggattctttt
    51661 aggtagcata attttaactt agaaaaaaca gctaactttt tagtttgatc ggtttctttg
    51721 gtttggatct ctcctttatg attagaataa ataaattcat caatcaataa aaattgcttg
    51781 agtgatacat ttatttttcg gatcagttct ttgattgtcc gttttggaat atgtaagtca
    51841 ttggataatt cttgtaaaga acaattattt ttttgaatga ccatttgtag aacaagtaac
    51901 tctttttttc ttgcttcaat tagcataaat acctcctttt tattctgttt ttgtatatag
    51961 ttagacaaaa actgtgagtg gaaagtcatt tcagtctgtg ttattctcac agagtgtcgc
    52021 cttctttttt atataacgtt atttaatttt tttgttacct tgtttattct attgtttatt
    52081 tcctagggta ttttgtcaag atattcttgt ttttaaggcg gaaaaattga tttttaatgg
    52141 atataactaa atatgttttg ataattatca ataatatttt tagaattatt tatgttattt
    52201 atttatgggt agatgaaaag agacggaggt aagaatgatg gggagagtaa caaattatag
    52261 taaagaatat ttgctgttca aaagtatggt atacgtggaa gaatatggaa tgaagtcaat
    52321 tactgcaaga gagttagcag agttctgcgg ttgttctaca tacccgattt atacacattt
    52381 caaatcaatc agcgggttaa aagagaagat attagaagaa atcactgttt attttgaaaa
    52441 ctacttagct gaatgcagtt ccgatgatgt tttaagcatt gtctacttat tgaaagattt
    52501 tttttctatc catgaaagta ttcgtgaaat cattgcaaaa tctgatttag acatggattc
    52561 gctatttaag cggccatttt tctcatatgt caaaggtcac acgaagatca aagaagaaaa
    52621 gtcgcttgaa ttggtatggc ttaatgcatt gggaagtttg cgctcagatg attcttctca
    52681 agattttctt gaaatgatga acgctgtttg ggataaaaac gaggtgacgg atgaatactt
    52741 ggaaaatatc ttaaaggaac agtcacctta ggcagcaaca tgaaaaagta gatcagccac
    52801 ctttattttt cgtaaaaaag attgtaaaat gtctaacgaa acattggaat gaaggaggaa
    52861 gcgacaacgt taccacagca atttatcaaa aaatatcgtc ttcttttagg cgaagaagca
    52921 tccgattttt tttctgcgct tgaacaagga agcgtgaaaa aaggatttcg gtggaatcca
    52981 ctaaaaccgg caggattgga catggtccaa acttaccata gcgaagaatt acaacccgct
    53041 ccctacagca atgagggatt tcttggcact gtcaatggaa agtctttcct ccatcaagct
    53101 gggtatgaat atagccaaga accaagtgca atgatcgttg gaacagctgc tgcagcaaaa
    53161 cccggtgaaa aagttttgga tttgtgtgcg gctcccggtg gtaaatcaac ccaattagct
    53221 gctcaaatga aggggaaagg gttattagtg acgaatgaga tctttccgaa acgtgcgaag
    53281 attttatcag aaaatataga acgatgggga gtttcgaacg caatagtgac aaatcatgca
    53341 cccgcagaac ttgtccctca tttttcgggc ttttttgatc ggatcgtggt tgatgctcct
    53401 tgttcaggag aaggaatgtt ccgaaaagat ccaaatgcaa taaaagagtg gacagaagaa
    53461 tctccgctat actgtcaaaa acgtcagcaa gagattttgt cttcagcaat aaaaatgcta
    53521 aaaaataaag gacaactgat ttattcgact tgtacgtttg caccagaaga aaatgaagag
    53581 atcattagct ggcttgtaga aaattatcca gtaacgattg aggagattcc tttgactcaa
    53641 tccgttagtt caggacgttc tgaatgggga agcgtagctg gtttggaaaa aacaatacgt
    53701 atatggcctc ataaagatca gggtgaagga cattttgttg caaagctgac ttttcatgga
    53761 caaaatcaga tgcataaaga aaagaagaca cgaaaaaaaa gtaaagtgca aatgacaaaa
    53821 gagcaggaaa aactctggac ggagttttca aatgattttc attatgaagc tactggaaga
    53881 cttttagtgt tcaacgacca tctttgggaa gttcctgagt tagccccttc tctcgatggt
    53941 ttaaaagtcg tacgtacagg tttgcattta ggagatttca aaaagaatcg atttgaacct
    54001 agctatgcat tagcgcttgc tacaaaaaag atagagaata taccttgctt gccgatcact
    54061 caaaaggaat ggcaatctta cacagcagga gaaacatttc aacgtgatgg gaatcaagga
    54121 tgggttttgc tagtattaga taagatacct gtcggatttg ggaaacaagt aaaaggaaca
    54181 gtaaagaact ttttccctaa aggattgaga tttcactgag tggattagtt cgtttatttt
    54241 tttgtctatg ctatgatgga gatagataaa aaggagctga tacttatgaa aacatcaaca
    54301 aaagtaacaa tcggattaag cgtagcagcc gctgccagtg tagcgacagc agtcgttgta
    54361 tcgggaaaag taatggaaaa gatccatcat atgactactc ggacgaaagt aaaaaaattc
    54421 gtctatgaca aatttgatgg aaatgaaaaa ttactagaaa tcgtagatgg tctttcagat
    54481 agtgacttgg attctttgat gggaatgtta gcaaaaatca aatcaggaaa aaagaaaatc
    54541 tctgtttatg gtgattcaat caaagattca accgaagatg taaaagaccg gctaatgcat
    54601 tttgttgaaa aaatgatgta agagaaacta gcttttgaac actattcatt cgaaataagg
    54661 ggttgtgaca aaattattgt cacaacccct ttaatctatc tttaattatg ttcatgaagc
    54721 aataaaagat agcaaaacag taaatcgttg gcttttttta gattgaaacc agactgttct
    54781 tgaaattttt cgattcgata ttttagagta ttacgatgaa gaaacagtgt ttttgcagca
    54841 gagctgacat tgcctaattc attccataat tcttgaatga tcacttccat ttcattttgt
    54901 ttgatcagtt ctatgtaact tttgatcaac ttactatctt ttacagtatc ttttgtatag
    54961 taatgtaaag ccacatcttg catagaaaaa gtacggctgt gagtataaag attgttttgc
    55021 tctgttaaaa aaatttttct ttcttcagca aacaaaggga cgaggtcggt gccaggtgaa
    55081 tggaaagaac caacgaatac agacgttgtc gtatcaaaat cagcatctaa agatagaaaa
    55141 ataccgttga tttcagtagc atcaagataa tcacggttta tttgttcgac taagagcgca
    55201 tctgtgtctg tataaaaaaa gaaatcagca gaatagcgaa ataattccaa aatagtatct
    55261 tgccattctt tttttaagaa ttctcctttt gattcgacgt gaagctggat caaacgaaag
    55321 cttttttctt gaacacaaga agcatcttta aaaagatagt gataccatgg gtgttttttt
    55381 tgatcgttga ttacagggaa taacgctttc aaaaggctgg tttcttgttg agataatgaa
    55441 tcttgtttga tccagataaa agcatcatca attgcaaaag aaaggatatc cgcttctgat
    55501 gctggagagt tttttttctg tgcttgtggg taaagagcaa gtaatttatc cattttcatc
    55561 ttgttcacct cttgtattta ttttaccata gaaaaaaaag ttagttacag cctttttatc
    55621 atatgctaaa gtaaaacaag aaaaaacaaa ggcagggaag ggtaattggt aaatacaaat
    55681 cttgatgcag tagaaaaaga aaaatggatg cgccttgcat tggcagaagc aaagaaagca
    55741 gaaatgcttc atgaagttcc gattggagca gtggttgtat tggatggaaa agtgattgga
    55801 agaggatata accttagaga aaccacgcaa gatgctacta cacatgcaga aatgcttgca
    55861 atcaaggaag cctgtgaaaa agtgggaagc tggcgcttgg aagatgctgc actttttgtt
    55921 accttagagc cttgtcctat gtgcagcggc gcgatgattt tgtctcgtgt agcagaagtt
    55981 tatttcggcg cgtatgatcc aaaaggcgga acagccggta ccttgatgaa tcttttggag
    56041 gacgagcggt ttaaccacca agcatatgta gaaggtggca ttttagaaga cgaatgtggg
    56101 atgattttga ctgacttttt ccgaaagctc cgccagcgaa aaaagaaatt aaaaaaagac
    56161 tggtcaacga atgaaaaata aggtatacta ttctttgccg aaaggctagg acaatggtgg
    56221 gcttgtgaag cgtgtcaggt ctggaaggaa gcagcactaa gcaggttccg ccatgtgtct
    56281 gattataatg aaacgacaac agactttcgg gtctgttttt tttattttta tagtactgta
    56341 aaaatgaatg ttcctgattt tttagccaga ctgatttatc gaatattgta cttatacaat
    56401 ttgcttcaag gcaggaaaaa ctctgaaata gagcttgttt caatggaaga aaaagctgcc
    56461 tatggtatga taaagataaa gagaggcgag agggtacatg gaaaaaatag tgattttaca
    56521 tacgaatgat ctgcattctc atttagaaaa ttggccaagg atcagaaggt ttttggatca
    56581 acgtaaacga gaaaacgaaa agaaagaaaa aacgacaacg attgcagtag atctaggtga
    56641 ttttgtggat cgttggcatc cattgtcaga agcaacgaat ggacaggaaa atatcttgct
    56701 gatgaatgaa gttggttatg atgcagcgac gataggaaac aatgaaggcg taggaaattc
    56761 caaagaagag ttgaatcatt tatacgatca tgctgagttt gatatcatcc tggataactt
    56821 attcgataaa aatacgctcc agccaccggc atggacgaaa ccatataaaa tcattacaac
    56881 gaaacaccag acgaaaatcg gactaattgc atttactgct ccatttccgc ttacttataa
    56941 tccgaatggc tgggacatac ggaatcctta tgatattttg ccagaattag tcgaagattt
    57001 acgttcacaa gtggatgttt tagttttgat gagtcatctc gggattcagg atgatttcca
    57061 aattgcaaaa gaaataccag caattgatgt tattttagga tcgcacaccc atcatttatt
    57121 cagagaaggt cacgtaatca atgatgtcca aatagcagcc gctggaaagt acgggcagta
    57181 tgtcggtgaa gtccatttaa ccatcgatga agagaaaaaa atccttaagc attcagccaa
    57241 agccatccct accgaaacaa tgacagcttt tacagaagat gaacaagaaa taaatagtta
    57301 tctagaacgt gggcatgaat tattaaaaga aaaagaagta gcagatttgc cattttctct
    57361 gagtctagat atcttcaatg agcattcgtt tatccaagta gcctttgagg cggtgaaaga
    57421 acgaggaaag acggaagcag caatattaaa cagtggtctg tttttaactg aaattccaaa
    57481 agggcgggtc aatcaggatc agcttcacac tgcgttgccg catccgatgc acttgttaaa
    57541 tgtcacattg ggtggaaatg atctgatccg cttagtttta gaaatagaaa aaaatcgtaa
    57601 ctttttacgt aactatccta tgaagggaat gggatttcgc ggtaagattt ttgggcagat
    57661 ggtctataat ggaatcacat atgattctat taaccatcaa gtcttttgga agaacaagcc
    57721 aatcgatctg aataaaaatt atacttttac taccgtagat catttgatgt ttgttccatt
    57781 ttttccaacg attgagattg ctggaaaaaa tgaatttttg ttccctgaat ttatccgaag
    57841 tgtggtagga gattacttga aagctcacta cccaatcaaa taaaaacaag gtataataaa
    57901 aaagataggt ggtgaacgca tgtctgcaaa acaaaatgaa cagcagaacg ttgagcataa
    57961 aacagaaatt gctaagaaaa aatcatcagc tgaacaggaa acaacagtca ccgaagaact
    58021 gacagctgct ttggaagaaa tcgttgagga gcctaaagca gaaaaagtca aaggcgagct
    58081 agtgacactg ttagatgaca gtaatttttt gataggtgaa agacattatc ggcttgtgtc
    58141 cgattatcgt gaaggattta atgcagaaaa gcttggagaa cgttacagtg atgtactagc
    58201 ccgatatgat tatatcgtag gagattgggg ttatgaacaa ctacgcctaa aaggattttt
    58261 ccaagcggat aatcgacggg cgcatccaga tcagcgaatc gattccttgg aagactatct
    58321 ttacgaatat tgcaattttg gttgtgcgta ttttgtcatc gagagaatcg gcggtaaaaa
    58381 agaaaagaca actcagcgcc gaaaaaagaa aaaaaaccat aatcgtgcgt atatcgatga
    58441 gaaaaaagga ccagtcgcat ctaatcgcaa aaaaccggtt attaaaaatc gaaaaacaga
    58501 gtcagcaaaa acctctgaaa aagtaaaaaa acaagaacaa cctaaacaag tagaaaaaac
    58561 aaaaacagca tttacgatca gaaaaaggga agagtagtgg atgaatggac tataaaggat
    58621 atttaattga tttagacgga acgatttatc gtgggacaca acccataccg gccggtaaac
    58681 gatttgtgga agaattgcaa aaaagaaaat tacctttctt gtttgttacc aataacacga
    58741 ctaagtcacc agaaacagta gctaatcgtc tagcagatga gtttgacatt catgtagctc
    58801 cagagacagt ctacacagct actttggcaa caatcgattt tatgaaagca gatggaaaag
    58861 ggaaaaaagt ctatgttatc ggagaagcag gattgattga cttgatttta gctgccggat
    58921 ttacatggga agaagaggct cctgactatg tagtggtagg cttagataat tatctgacct
    58981 atgaaaaagt agttaaagca acacttgcta ttcaaaaagg tgctactttc attggaacga
    59041 acccagataa gaatatacct actgaacgcg gccttcttcc tggagcaggg tcagtcatct
    59101 cttttgtaga gacagcaacc caaacgccgc cgatctacat cggaaaacca gaagccatca
    59161 ttatggataa agccgttgaa gtattagggc tgcaaaaaga agaagtaatc atggttggag
    59221 ataactatga aacagatatc caagctggga tacgcaataa tatagatact ttgctcgttt
    59281 tatcaggttt tacaaaagaa gaagatgtac ctgggttgcc tatcccagct acctatgtga
    59341 aacaatcact ggatgaatgg agcttctaat atgaaaaatt cgagatggca gtggatggaa
    59401 tatgctggat tgttcagcct atttctgaca ctgatcagtc tggcagttgg tgtgacgatc
    59461 aactttcgac cactatacgt atttgatatc ggacatttgc aaattttgga ttatacttct
    59521 ttagatcaag agacgttact gaaaaatttt gatcacctaa tgaattatct caataatccc
    59581 ttcaaaacca tcctcagctt gccagatttc ccagtttctg ctagcggagc ccatcacttt
    59641 tatgaagtga aaattttgtt tttagtggat tatgctgtgt ttttcatcac tttgatcccg
    59701 agtattttgt ttatcaaata tttgcaaaag aatgatcggc tttggcggtt gattcgtccg
    59761 tttcaaatcg gcatgttact gccagttgtt ttcggttttt tcatgatgat aggatttgat
    59821 cgctttttta tcctttttca tgaaacgttt ttcaacaacg atgactggtt attcgatccc
    59881 gttactgatc caatcatcaa tgtacttcca gaagaatttt tcatgcatag ctttatctta
    59941 ttttttgtat tattagaatt attttttgct gtttttcttt tcttaggaaa aaatagcttg
    60001 aaacaaacaa aaaagaaaga actcgtctaa atagacgttt tctttctttt tttgttgaat
    60061 tattgagtaa ggcgacttct tttttctggt tctttgtgtg ctaatcgact ggcagcagca
    60121 gcggcgatcg ctccgacgat atcatctaag aaggtatgga catgaacgcc atcgtggctg
    60181 tttagttctt ttaaaatacc aggtttcacc ttgtcgatat aaccataatt cgtaaagccg
    60241 atcgtaccgt aaacattcac gattgacaag gctaggatct catcgatacc gtaaaggcct
    60301 tcgtcatctt cgataatatg ttgcaacgga ctcatcaatt ttttcttttc tgctaatata
    60361 tctaattgga ttccagtgat aatggcgtta tgtacttcac gctttgttag cacgctgttg
    60421 acactttcag tacaatccgc caaagtcaaa tcagaaatat agtctttttg taaaaacatg
    60481 acaagttgtg cgatatcttc tacagttacg ccacgttcct tcaatagttc tcttgccttt
    60541 tcttctaaag tctcagtttt gacgaccata ttattgctcc ccctttattt tttctttctc
    60601 tatttcatgt taacggaaca tgaaaaaaat gcaagaaact gctttcgggt ttaacttgaa
    60661 atgcaaagag atataaaaaa tcaactagga cgaagatttt tatcgtataa gcttgtgctg
    60721 tgaccaggct gagttcgttc ctttggatta atatagtgta aagcgttgtt cactgcagta
    60781 ggtgcttcac cgagcccagt agcaatcagt ttcacttttc cttcataggt acagatgtca
    60841 ccagctgcat aaacgcctgg aatagaagta gacatatctg attttacagt aatggcattt
    60901 ctggtactat caagtcccca agaagaaaga tgttctaaat tagaagagaa tccataattg
    60961 acgatcagag aatcaatcat caaatcaatc gtctcgtcac ttttcacttt ttttaaccgg
    61021 atatctgtca gttccccatt attgcctgat aatccatcga tgagataggg agtaagcaag
    61081 ttgacagatg aagatttcaa gcgtgataca ctatgttcgt gcgcacgaaa ttccgggcga
    61141 cgatgaatca agtaaacttc gcttgcaata ggctctaaca tcaacgtcca atcgatagca
    61201 gaatcaccac ctccagcaat agcgactttt tttccagcat acttcatcaa atcattcacg
    61261 aaataatcca atccgtggtt ttcaaatgat tcagcattat ctaaattcaa ttttcttggc
    61321 tggaatgaac catttcctaa agctaaaatg actgctttgg aataatggat gcctttattt
    61381 gtagtaatct cgataacttc gtcttcacga gtaagcgaga gaacttcttc ttttaaatgg
    61441 aatgtatggt taaaagtagt taattgtttt tctaattgat cgattaattc acttgctttt
    61501 attgctggat acccaggaat atcgtaaata tatttttcgg gatacaaagt tgctaattga
    61561 ccaccaagtt gaggtagact gtcaatgatt tttgttttag cttggcgcat tcctgcgtaa
    61621 aaagcggcaa acatccctac aggacctgca ccgatgattg taatgtcata gatttccata
    61681 aaaattcctc ccttttcaca ctatagcaga atttaattga gaatgcttct caaaaatgtt
    61741 tgaattcgaa atcgaaacgg taagttatag aatgtatcta gtgatgaaaa ataaaagaaa
    61801 acaaatctag tatttttacg agaggttagg tattatgtta ttattattat gaagtatgca
    61861 aatattagga ggaaacaata tggcattccc acaactagat ttaaaaagtg aaaaaggacc
    61921 aaaagctgtt atcaaaacaa accgtggtga cattactgtt caattattcc ctgaactagc
    61981 accgaagacg gttcaaaatt tcattgaact ttccaaaaaa ggttattatg atggcgtgat
    62041 ttttcaccgc gttattcctg atttcatgat ccaaggcggc gatccaactg gaacaggaat
    62101 gggtggcgaa agtatttatg gcgaaagttt tgaagatgaa ttcagtcgtg aactgttcaa
    62161 ccttcgcgga gctttatcta tggcaaattc aggtccaaac acaaacggaa gccaattctt
    62221 tattgtaaat aacacaaatg tgccagctaa tatgcttgga caactagaag gtgctggatt
    62281 cccatcagaa atcattgaag cgtataaagg tggcggaact ccttggctag atttccgaca
    62341 tacagtcttt ggacatgtac tagaagggat ggacacagta gatgaaatcg caaatgtaca
    62401 acgtggtcca caagaccgac ctgtacatga tgtagtgatt gaaacaattg aaatttcaga
    62461 atagtttggt atggtacgga gtaattatga aaaaggacga aacctagtaa ggtctcgtcc
    62521 ttttattttg aataaatgat ttaatcgatt gtctctaaag catacttgat tcgattaagt
    62581 ccttccagta aagtttcttc tggacaagca atattcaagc gcatatgccc atgaccggaa
    62641 ggaccaaacg tgatgccagg atttaatacg acttttcctt tccgaacaag agtgttctcc
    62701 aattcttggt cgtctgaaat ataggcggta aaatctaacc acattaagta agttccttca
    62761 gggttcatta ttctcacttt cggaagatac tgttcaaaaa actgattaac tgtttgaaca
    62821 tttttttcta gataaggaat cagttggctt aaccattctc cacccgtctc ataagccgct
    62881 tgggttccaa tcagtccaaa agtgttgatt tcgtgctgtt ggttcatcac taaatgtttt
    62941 tcaaaatcag ctttcatctc tggatttttg ataaatacca ttgaattttt gattgcagct
    63001 agattgaaag tcttggttgc ggaagtaaaa gcaactagta gatcatctaa attaggatca
    63061 atcgttagca tggaagtaaa tgtatgatcg aataaagtta aatcttgatg gatctcgtca
    63121 ctaaacagaa tcacttgatg ttttaaacac aattcgctta actgtttcaa ttcttctttg
    63181 ctccaaacac gccctccagg attgtgagga ttgcataaaa tcattgcttt tacattgttt
    63241 tcttcgattt ttttctccat gtcagacaag tccatgatga aatgattatt tttttctagc
    63301 aaagagcttc gaacgatgtg acgttgattt gtttctataa tagaggaaaa tggaggataa
    63361 actgggtcat gaatcaaaat agaatcgcct ggttttgtaa aagtttgtac tgctgcagct
    63421 aaactggtta acacaccact tgtaaaaacg atattttctt tcgtcaactc cacagcatgg
    63481 tgttggcgtt cccaatgaat gatagcctga tatagtttat cagacagagt ggcataacca
    63541 aagattccat gtttgatgta ttcactaaac gctttagcta ccccaccggg agacaagaag
    63601 tccatatctg ctacccacaa aggaagtaag tcatccattt gatatgtctc agcaattgcg
    63661 tcccatttta cactttctgt atcttttctt gaaatctgtt tatcaaattc gatcatctta
    63721 agacctccag tcattttcgt cgcttctttt tttgagctat ttacagtata atgataaatg
    63781 cgaagagagg gaagtagatg acttataaaa ttggacaaat aattgaagga aaagtgacag
    63841 ggatacagcc ttatggcgca ttcgtttctc ttgataacga gacgcaagga cttattcatg
    63901 tttcagaagt acaatccggt tatacgaaaa atatccattc attgttaaaa gtcggacagc
    63961 cagtaacagt tcagatcatc gacattgatg aatattccaa aaaaatcagt ttatctttaa
    64021 gaacgttaga aaaaacaaat ccagctgttc catatcgcag aaaaaggtat ttcacgaata
    64081 aaaatcgtaa gatcggattt accactattg cggagcagtt acctatttgg atagaagaag
    64141 cattatctga actagaacgg aaagaaaata agaaataaaa agtgtatttt ttctgaaaat
    64201 gtgctagaat ttttatgtaa gccatagaac aagttgatcg aggtggaaat tttggcaagc
    64261 aaaagagttg ctggtacaat tatgttgcat ttggaggatg gtactaaaaa atttttggtc
    64321 cattcaattg atgacaagtt ggaatttgcg ttagcggaac tttctgaagg aaagactggt
    64381 ttagcgaata tacttaattt cttaaaagaa atagttcata tcgatgttag caagattagt
    64441 ttgatggaat tgacaaatac acatattaaa gaggaaaatg ttccgttgtt tgtctttgaa
    64501 actgaacaaa agaatcaaag agaagaatta ggcgaaggat atatttggga agaaccaggt
    64561 caaatgcgct ctgttcttgg aacttacgaa gtagagggag tgcctttctt ctaaaataca
    64621 gattaagcga acaaagaagt ttgaattttt ttcaaacttc tttttttatt gtttggtcca
    64681 aaaaatacaa tgaaatgttc tgctaaagat agttttttag tgcattttat tttcaaaata
    64741 tgttgaaaac ggttgtataa cgaataataa taattgatcg aaaaatcatt tcacaaagta
    64801 atgcttaatt tatagttgac caaggaagaa atacatgata aatttaacag cgttgattct
    64861 gaagtggatg ttatatcaca tttctaaaaa atttaaaaaa tagttattga catgttacta
    64921 acgacatgat aagataactg agtcgtt
//
LOCUS       DACGLE010000007        64193 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0534
            SAMEA3304449-rid9560453.denovo.007, whole genome shotgun sequence.
ACCESSION   DACGLE010000007 DACGLE010000000
VERSION     DACGLE010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304449
            Sequence Read Archive: ERR985064
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 64193)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 64193)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702225.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702225.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 20:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,045
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 27 of 67
            Pseudo Genes (incomplete)         :: 25 of 67
            Pseudo Genes (internal stop)      :: 19 of 67
            Pseudo Genes (multiple problems)  :: 4 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..64193
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304449-rid9560453.denovo.007"
                     /strain="VREN0534"
                     /db_xref="taxon:1352"
     gene            complement(137..1528)
                     /locus_tag="IXT20_000552"
     CDS             complement(137..1528)
                     /locus_tag="IXT20_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine transporter"
                     /protein_id="HAQ4859027.1"
                     /translation="MTTLITVLVVLVFAALLYVFYRMQKRHTKFSTRVFTALGVGIVF
                     GGLLQLIFGVDDKVVTQSMEWIGIVGQGYVSFLQMLVMPLVFISIIGAFTKMKVSEKL
                     GKISATVLTSLLGTTAIAALIGIGSTMLFGLQGAKFTQGAAETARISELATRQETVEN
                     LSIPQQILSFIPKNVFADLAGSRATSTIGVVIFAAFVGIAYLGVKRKAPKEAAFFSDL
                     VDSLYKIIMRIVTLVLRLTPYGVFALMTNVLATSDFAAIVNLGKFVVASYVALLAVLI
                     VHSLILIGAKVNPINYFKKAFPVLSFAFTSRSSAGALPLNIETQTKALGVDDATANFS
                     ASFGLSIGQNGCAGVYPAMLATIVAPAAGVNVFSLEFILMLVAIVTISSFGVAGVGGG
                     ATFASLIVLGAMNLPVAIVGLVISVEPLIDMARTAVNVSGSMVAGIVTSARIKDLDRD
                     VINDDSKVIDVNA"
     gene            complement(1741..2127)
                     /locus_tag="IXT20_000553"
     CDS             complement(1741..2127)
                     /locus_tag="IXT20_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1149 family protein"
                     /protein_id="HAQ4859028.1"
                     /translation="MEIRRQKEVVEAFHYDMRLPNQEVETDLKVGFSPLKETIENYPK
                     EHSIVGARLEFRLVFDTYVLSGSVSQINHILNRKIEGQNDVTQEEVDELVAPLFDIVR
                     RMSYEVTEIALDQPGVKLNFQSEKQE"
     gene            complement(2183..2806)
                     /locus_tag="IXT20_000554"
     CDS             complement(2183..2806)
                     /locus_tag="IXT20_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HAQ4859029.1"
                     /translation="MKNYIWDFDGTLFDTYPAMVDGAWQALKDFGISMDKKEIYFKMK
                     KYSTSYLINESNLNAREFNELFHRYEKESTEVSRPFPETKQVLEMLKDNGGRHFILTH
                     RLTESTWGLLKEHRLAHLIEEVVGIDQDFPRKPDPASLNYLIDTFHLERTDTMMIGDR
                     RLDIEAGKNAGVATCLYDIDHFLGEIPADYVVGNLNEILTLQSIIKK"
     gene            complement(2870..3538)
                     /locus_tag="IXT20_000555"
     CDS             complement(2870..3538)
                     /locus_tag="IXT20_000555"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HAQ4859030.1"
                     /translation="MKLVLLRHGESEANFENYWTGWLDVALTEKGKEQAAVAGQKMLE
                     AGLQFDAVYTSVLKRAILTGQVALEEMGQLWLPIIKTWRLNERHYGDLVGKNKDKMKE
                     IYGKDQVKTWRRGYTEVPPLTAENRFDRRYDHLDPHLIPYGESLEMTVNRIIPLWQDH
                     LAPKLKDRQDLLVVGHGNSIRALTKYLEDVPEDQMDTIDIPNAQPIQYTLADDLTILD
                     KKIL"
     gene            complement(3550..3990)
                     /locus_tag="IXT20_000556"
     CDS             complement(3550..3990)
                     /locus_tag="IXT20_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4859031.1"
                     /translation="MMQTWLERLIRFQQNYHKLMQGRYAKFDQLNRGLLIAALVFSLL
                     SRWLPYRIGQLLFIVFFGWMIFRFLSKKIYPRLNENQRYLKYLDRVKEKYHQIKNKKQ
                     VKMDRKSYTFFECPNCHQKQRAPKGKGRIRVTCKTCGIKFETNV"
     gene            complement(4049..4720)
                     /locus_tag="IXT20_000557"
     CDS             complement(4049..4720)
                     /locus_tag="IXT20_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4859032.1"
                     /translation="MNFLVVLKDTLIERSTFLYVQLQENKTLLHFLPEFHLEGLTLGE
                     IYQAEGLSAVLHFFKVELELPVKDYIELSLESWDRAVVELFPEGLMIDTNDGKIHLTA
                     AELQKQMRYQIDDENSFSIFRRQQKILKSFLKVISKKRNLIKTTQLLKKYPGLLHTSI
                     QFPQLITMIHRFIRMQQLPSKKLFLPLTDTFRVVNREDKKKVIVIDFTRNRNVLHQVA
                     MEKAN"
     gene            complement(4905..5303)
                     /locus_tag="IXT20_000558"
     CDS             complement(4905..5303)
                     /locus_tag="IXT20_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HAQ4859033.1"
                     /translation="MKDLVALNLHMIVTIVDGGMGEDVIDFSKEAGAAGGTILHGRGS
                     GVHDSGKFLGLDISPEKDIVLTLVPDSLTNKVMDAIGQGINIDIPGNGICFSIDVDKV
                     IGITKIHQYREIIGMQELLDENNDREKSES"
     gene            complement(5300..6071)
                     /locus_tag="IXT20_000559"
                     /pseudo
     CDS             complement(5300..6071)
                     /locus_tag="IXT20_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331418.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
     gene            complement(6068..6751)
                     /locus_tag="IXT20_000560"
     CDS             complement(6068..6751)
                     /locus_tag="IXT20_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HAQ4859034.1"
                     /translation="MLKNFKEVIAAILPMTVLIVILTFVFAPLEGEDLASFLVGAAIM
                     MIGMTLFLFGADYSMMEVGDLVGKYMIKKKSLTVLVSLGFAIGIVITIAEPSVQVLGQ
                     QVYDISDGEIGRVLLIGIVSVGTGVFLAFALLRVVFKLSYYQLMAIGYIGVLIASFFT
                     SSEFMPIAFDSGGVTTGPVTVPFILALAGGMTSMIKQKKNENDSFGMVGIASLGPILA
                     VMILGVIFQ"
     gene            complement(6918..7805)
                     /locus_tag="IXT20_000561"
     CDS             complement(6918..7805)
                     /locus_tag="IXT20_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAQ4859035.1"
                     /translation="MKNMVEKETIERPNVKKQALELRSTIGSVLKYTALSILSFISIF
                     PFVWMVLGMTNTPVDITSGKIKIGSNLIINFQNLFSNELNFTRSLWNSAMIALVTTVF
                     ALLISSMAGYGFEIYQSKRKDLIFNILLLSMMVPFAALMIPLYRMFSQLNGTVLGINS
                     FLVVILPSVSTAFLIFFFRQNVKSFPKDLVEAARLDGLKELSIFFRVYMPTAKNTYAA
                     AAIITFMSSWNNYLWPLVALQSPDKRTVPLVLSAMGASYTPDYGMIMAGLVIATLPTA
                     IVFFVLQKQFVQGMIGSVK"
     gene            complement(7802..8671)
                     /locus_tag="IXT20_000562"
     CDS             complement(7802..8671)
                     /locus_tag="IXT20_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ4859036.1"
                     /translation="MKQKDLFKNGHAFLILPVILISIMVFIPMVLALITSFQSGPPVN
                     MTFNGLGNYQRMLTDATFKKAFFNTFLYLLVQVPIMLFLALFISNLLNDKKLKYKGLF
                     RTAIFLPCITSMVSYSLIMKSLFSQSGLVNNLLLGIGMIDTPIGWLTHPIWARVLIIL
                     SITWRWTGYNMIFFLSGMQNIDSEIYEAASIDGASKWQQFIKVTIPNLKPIILFTSIT
                     STIGTLQLFDEVQNITGGGPANATTTLSQYIYNLSYKFTPNFGYAAAVSFVIVSFIVI
                     LSIIQTKVGGESR"
     gene            complement(8808..10094)
                     /locus_tag="IXT20_000563"
     CDS             complement(8808..10094)
                     /locus_tag="IXT20_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HAQ4859037.1"
                     /translation="MNIRKLIAGVVCLAAASGFLTGCGSASSTADKEEKVTVWAWDET
                     FNIKAVNEAKKVYENEETEIEVVTMSQDDIVQKLNTALASGNTEGLPNIVLIEDYRIQ
                     GYLTSYPDAFANLSAIVKEDHFAPYKFAVNKVGDKIYGVPFDSGVTANFYRTDLMAEA
                     GYTEEDMENLKWDEYIQVARDVKEKTGKKIAEVNPSDLGRVRMIMQQAGEWYTSEDGE
                     TVTIQDNASLKYGLELFATLLKEDLVEQTSDWNAGVTAIQSGAVASSPTGAWYSSTIQ
                     GAENQSGKWKIAPIPALPENLQKAQASNLGGAGWYVIKGVAGEDSAKDFLEATFASNE
                     ELMGTLAKEIGLVSTMLSANEQEAYQEASEFYSGQKIFDDFSTWTSEIPEVNYGHETY
                     AIEAVVAEALHRIINGEETDKVLADTQKQVEAQLAN"
     gene            10367..10945
                     /locus_tag="IXT20_000564"
     CDS             10367..10945
                     /locus_tag="IXT20_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HAQ4859038.1"
                     /translation="MPIRVILIDDEQTILDGMKQLINWSEHGFQLIGTFSSPLKALDF
                     SLVHAIDIVVTDVMMPELNGIELSRLLKQQQPETQILILSSYDEFDMVKDSFKEGVAD
                     YLLKPRLSPDFFLNSLTAVSQKIKKKPNKSPITNRYEQICENLSQQIAGQTRPLLDPL
                     LFEHTLFFCYIPKNGHSVQNKALNKNSRSPIH"
     gene            10945..11874
                     /locus_tag="IXT20_000565"
     CDS             10945..11874
                     /locus_tag="IXT20_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ4859039.1"
                     /translation="MDSDDSFTIYPVSTFSEESGYVINTDSKDTLRKFVQKFRQEVFP
                     GDFFVVSDSFAIQDINEIFQEIRNCTKGQRFYHKGKNLAYQHELLPLLTNHEQQASSY
                     LTKIVHKDYALAVKELENFFTTILEQPIDPAYLKHEAINRFYALLNALADEHVGNEEL
                     SLLKIALPSLLADAEYFEDFSILVKETLEKLTHFSANAKKDNFDILTLIYEFVQENYE
                     KEINLQLLSEKYHFSYSYLSAIFTEKFGINFSKYLKKVRISKAKLFLTETRLTLTEIC
                     EKVGYTELGYFSRVFKEETGMTPSQYRKGTFVK"
     gene            11871..13607
                     /locus_tag="IXT20_000566"
     CDS             11871..13607
                     /locus_tag="IXT20_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAQ4859040.1"
                     /translation="MRKKIKEHELFAKLLFILFIGLFLQAIVISLFIYHRSRDAYIQL
                     FNQSNDVVLKKIQSEFESLNDTIENTLAAFDSNPAVKSYFSNDPAQHMEQLQQLRTIQ
                     KMNDSLSKIHPMIDYDVLIFGENGRTFVGNDMLTAVSADSFFQSAIAQRVNERAADTQ
                     MLFAHHGLTLRDKKAPSVFFVKKLKNTLNHVYGYAVLSISSKQLANLFQSVVNPEISK
                     ISFMNEEQTIIASNEENTIGSRSMIFKKLTVGETTISNGQQLTRLPLYRQDCTLLSQV
                     DLHSLSNQMGVIFPIILYNIVTLAFAGSLVFMYLDRHTKSIYRLIDSLKRLEEDGLKA
                     TVPVQGSHEVQLLGNTINQLLSNAQHHYEQTIQDEKKKRSLEIQTMQAQIQPHFIYNT
                     LTSLKFLIWQKKTDEAVQGIDSFITLLRSTIGKKEEVIPVKEEIKSVQSYIDLLSMRY
                     GERISAKIMIPDELQSLSMPNMILQPIIENAYLHAFHNKTQGFITVYGKTKNQLLLFE
                     VIDNGDGFDTENTKQKNDHFSGIGISHVDERIRLMYGDAYGLTIQSVIGTGTTVTICL
                     PLLPKNQSEYQK"
     gene            complement(13670..14506)
                     /gene="pheA"
                     /locus_tag="IXT20_000567"
     CDS             complement(13670..14506)
                     /gene="pheA"
                     /locus_tag="IXT20_000567"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HAQ4859041.1"
                     /translation="MNVSYLGPESSFTYQAACQLFPTEQLTAYASIPVCLRALFRKQV
                     DLAVVPVENSLEGAVHHTIDLLSKHPEVEVKSEIVLPIKQQLLGNPATKITKILSHPQ
                     ALAQSQQFLETHYPNVPLVATESTTAAAMYVAEHPKEDAAAIASLETAQHVGLEILAE
                     NIQDNELNQTRFWIVGDRKMTSQQSAPVKMSVILTLPANRPGMLHKMLAAFGWREINL
                     SKIESRPLKTSLGEYFFVIDLLLDRPMTLVENALQEIKMLGGESQILGCYPVLTVEET
                     RR"
     gene            complement(14503..15012)
                     /locus_tag="IXT20_000568"
     CDS             complement(14503..15012)
                     /locus_tag="IXT20_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HAQ4859042.1"
                     /translation="MASILLIGFMGAGKTTIGKGLAQCLQKEYVDLDTKIEEHIQLSI
                     AEYFHYYGEKSFRKVESDILRKLSNEDKIIATGGGIVQSAENRRFLKTQPIVLYLEAE
                     ADCLVDRIQQDETSIRPLALGKTREEIKALLAQRLSWYEESATHRINTTNQTPEKIID
                     TIIERIKSI"
     gene            complement(15005..16300)
                     /gene="aroA"
                     /locus_tag="IXT20_000569"
     CDS             complement(15005..16300)
                     /gene="aroA"
                     /locus_tag="IXT20_000569"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HAQ4859043.1"
                     /translation="MQLLQQIHGLRGTVRIPADKSISHRSIMFGAIAEGTTTIQNFLR
                     AEDCLSTLHAFQQLGVEIEEEEEVIKIHGRGSHSFVQPTESIDMGNSGTTIRLLMGIL
                     AGQPFTTTLFGDASLSKRPMGRVMEPLREMGADLQGEENDQYLPITVTGTRSLSPIRY
                     NMPVASAQVKSALLFAALQAEGTSVIVEKERSRNHTEEMIRQFGGRITVEDKTIIVTG
                     PQKLTGQQITVPGDISSAAFFLAAGLLVPESQLLLKNVGVNPTRTGILDVLEEMGAAI
                     TQTNHNEQNQSADLSVKTSHLKKATIGGEIIPRLIDELPILALVATQAEGITIIKDAE
                     ELKVKETNRIDAVAEELQKMGAKIKATADGLIIHGPTPLHGAKVSSRGDHRIGMMLQV
                     AALIADGPCELEGAEAVSISYPAFFDDLAELVSGGEAHG"
     gene            complement(16313..17404)
                     /locus_tag="IXT20_000570"
     CDS             complement(16313..17404)
                     /locus_tag="IXT20_000570"
                     /EC_number="1.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HAQ4859044.1"
                     /translation="MAQHVFIVGLGLIGASMALCIRQTNPELIVVGWDSQSKTREQAV
                     EQQIVDYVASDFETGAEQADVILLAVPVRTTLAYLDVLERINLSDHVIITDVSSTKQQ
                     VVTSAEQKNLRFVGGHPMAGSHKSGIQAADAKLFENAYYIFTPLEKTKKDVEKLQELF
                     CGTKAKYVTLTAREHDRITGMLSHFPHILAAGLVNQAEQFNQEYPRAKQLAAGGFRDI
                     TRIASSDPVMWTDILLSNKQILLERLADWQQEMTQIAEWIMTENQSEIFSFFNRAKES
                     RDQLPVHKQGAIPAFFDLFIDVPDEPGVIAEVTGLIGKAGVSLINLKIQETREDILGI
                     LQISFKNQQDLLQAKRIIMSNTSYHCWIK"
     gene            complement(17421..18587)
                     /gene="aroC"
                     /locus_tag="IXT20_000571"
     CDS             complement(17421..18587)
                     /gene="aroC"
                     /locus_tag="IXT20_000571"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HAQ4859045.1"
                     /translation="MRYMTAGESHGPELTAIIEGLPAGLPLSEEEINHELARRQAGYG
                     RGGRMKIEKDRVRITSGIRHGKTIGSPVTLVVENKDWKNWTTVMSVAEVPEKDQSLRR
                     VARPRPGHADLVGGMKYHHRDLRNVLERSSARETTMRVAIGAIAKKILEELDIIVAGH
                     VICLGGIQAEIPGNISIQEIKEISEASEVRVVDPAVEQRIKDLIDQTKKNGDTIGGIV
                     EVLVGGVPAGLGSYVQWDTKLDAKIAQAVVSINAFKGVEFGVGFTSGSLPGSEVMDEI
                     LWEEERGYFRRTNNLGGFEGGMTNGEPLVIRGVMKPIPTLYKPLQSVDIDTKEPYKAS
                     VERSDSTAVPAASVVAENVVATVVATELLSKFPSDSLVELKAAVKEYRDYLKHY"
     gene            complement(18589..19653)
                     /gene="aroB"
                     /locus_tag="IXT20_000572"
     CDS             complement(18589..19653)
                     /gene="aroB"
                     /locus_tag="IXT20_000572"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HAQ4859046.1"
                     /translation="MLTVHVDNQKYDIHIEKGALEQAGSWLQTIWRPQKVAVITDTNV
                     APLYAEKIMFQLRQANFEPVLSIVPAGETSKSLEQAAELYDFLADNDFTRSDGILALG
                     GGMVGDLAAFVASTFMRGIHFVQIPTTLLAQVDSSIGGKTAVNTNKAKNLVGTFSQPA
                     GVLIDPEVLKTLPVRRLREGIAEIIKSAAIADATLWKQLNELKDEKDLLEHAVPIITA
                     TLEIKRKVVEEDVFDQGNRLILNFGHTIGHAIENTAGYGVVSHGEGVAIGMVAITSHA
                     EKIGLSPTGSTAALERILQKFHLPITYDLSHPDELFHAITHDKKARGSQLNIILLEKI
                     GQAKIVPVPTETFKDYLGGL"
     gene            complement(19686..20708)
                     /gene="aroF"
                     /locus_tag="IXT20_000573"
     CDS             complement(19686..20708)
                     /gene="aroF"
                     /locus_tag="IXT20_000573"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HAQ4859047.1"
                     /translation="MIVIMKKEATEEQIKKVVDHIEKEGFKVSIDQGTERVVIGLKGD
                     TRSLQEGAFTRYEGVENAVRILNTYKLTSREFHPGNTVVDVDGVKIGDGSFVTMAGPC
                     SIEGLDQIRECARMAKAGGAKILRGGAFKPRTSPYAFQGLEEEGLKYIRQAADEFGMK
                     VITEVMDEGHIDMVAEYSDILQIGARNMQNFKLLSAVGKTGKPVGLKRGISGTINEWL
                     NAAEYIAVEDKSPVIFIERGIRTYETATRNTFDLSAVPLMKKLTHFPVIVDPSHGTGI
                     WELVPPMARAGVASGADGMIVEIHPDPANAWSDGPQSLNEKTYSRMMKEVAIMKEAME
                     KIQTLD"
     gene            complement(21367..22341)
                     /locus_tag="IXT20_000574"
     CDS             complement(21367..22341)
                     /locus_tag="IXT20_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase small subunit"
                     /protein_id="HAQ4859048.1"
                     /translation="MKNTVNKSHMDTEKVAIVFGDCTLGVKSGNTHYIFSYTRGGLES
                     LNKNGKEWLYRETLPVFWRATTDNDRGSGFGFKSGMWLGAGLYPKVKEIEVLVDNKKI
                     DLPIAPVNNQYSSNEYAETVEIIFTLEINTVPKTQVTISYQIAGEGKMLVNMHYYGQK
                     GLPELPALGLRFIMPTKAKFFEYEGLSGETYPDRMAGGVEGIYKVAGLPVSPYLVPQE
                     CGMHMETKWVELTRNQTLDNTDKDQEDFSLRFEQQDTPFAFSALPFKAEELEQATHIE
                     ELPPERRTVLTIFAKVRGVGGINSWGADVESPYRISSEEDHEIGFIIQ"
     gene            complement(22322..24205)
                     /locus_tag="IXT20_000575"
     CDS             complement(22322..24205)
                     /locus_tag="IXT20_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2"
                     /protein_id="HAQ4859049.1"
                     /translation="MKANIKWLDDPQVFRVNQSNAHSDHVCYATREEVETTSSLKQSL
                     NGSWRFCYSPNPSVRPKTFYKKDFDACSFDEITVPSHIELANYDKIHYINTMYPWEGH
                     VFRRPPFTTSPEKEAEGIFSAAKDNPVGSYLTTFRLEEGLKGKQISIHFAGVEQAMFI
                     WLNGHFIGYAEDSFTPSEFDLTPYLEEENILAVEVYKRSTAAFLEDQDFFRFFGIFRD
                     VFLVARGQNHLEDLWIKPRLDDKCQNGKLEVDLSFEQVAKNSQLNVAILDPDGKEIYQ
                     QQYPLKEQMTIRIDGFENVALWGHFQPNLYKLELETIDEEKRVTEYIDYSFGFRKIEI
                     KDKIIYFNGKRMIINGVNRHEWHPKKGRAITIEDMEKDLAIIKNAKINGVRTSHYPNQ
                     ITWYYLCDQAGIHVMAETNLESHGSWQKMGAFEPSYNVPGAVPQWQAAVLDRAKTNFE
                     TLKNHPSILFWSLGNESYAEENIRQMNDYFKKVDPDRLVHYEGNFPNPAFEDSISDVK
                     SCMYAPPEEIIDYLENKAEKPFILCEYMHDMGNSLGGMDSYMTLLDRYEMYQGGYIWD
                     FKDQAIQVTDPITGREVMRYGGDFDDKPSDYEFSGNGIVFADGQEKPAMQEVRYYYEK
                     YSK"
     gene            complement(24223..26448)
                     /locus_tag="IXT20_000576"
     CDS             complement(24223..26448)
                     /locus_tag="IXT20_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HAQ4859050.1"
                     /translation="MDITHRKTDKNFILFDEKSRVFHLRNTFLSYLIKIEESNVLAHI
                     YFGKPVKQYKDNKNYPRRDRGFSGNVPLNPDRSLSKDTLPQEYSSHGSMDFRTPASII
                     QRKNGSDLLDLRYDSHYITDGKPEIEGLPQTYVMDKSEAQTLVISLKDRETAIYFDLF
                     YTIFTDRAVITRSVKIRNETGETIKLEKAASFQLDFAHTRRFDEVIALPGAHVNERQI
                     SRQSVLSGTKVFESRRGTSSHHMNNFIALVHHHTTENTGEAIGLQFVYSGNHSFELEK
                     DQINQLRVVGGINSHRFSWELNAGQSFQTPEMILSYSSQGLNKMSQIHHELLRERIAR
                     GRHQFAERPILVNNWEATYFDFNSEKIKAIIDEAKELGIEMFVLDDGWFGKRDADNSS
                     LGDWFEYEGKLTNGLREIADYAHSKGLKFGLWFEPEMISVDSELYRTHPDFLMQEPGR
                     MPSASRSQHVLDFTRLDVRQTIEKQMRKILDTIPLDYIKWDMNRSLSDVYSITLDPQR
                     QGEVAHRYMLGLYELLEHLTTDYPEILWEGCSGGGGRFDAGFIYYMPQSWTSDNTDAV
                     ERMKIQYGTSLAYPISSITAHVSAVPNHQTDRSTSLKTRGETAMSAVFGYELDLTKLS
                     PEEEKQVKDQIISYQTIRPVIQYGHYYRLASPFEENIAAWMFVSPKQDEAIVFLGRIL
                     ASAQPAFHEVYLMGLDDEALYQEQTSKRIFSGAELMTVGLYFPDFQGDFQTELLHFKK
                     L"
     gene            26563..27597
                     /locus_tag="IXT20_000577"
     CDS             26563..27597
                     /locus_tag="IXT20_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAQ4859051.1"
                     /translation="MVGIRDVARKANVSPATVSRVLNNDQTIHVAQTTRQRIVEAAAA
                     LDYDISTRKYIKKRMPSIGVISTIGKASEEQDIYYKHLRVGMEEEARRLHLGMNRIYN
                     LSDNPKQWEDLDQLGAVIVVGTVTKESITALLKQNMQIVVVDNPDIQQEVDMVYADLE
                     RMTKTVLELFLENGHKRIAYIGGYKVDMDEFGGKQISINEKRMRAYQHFMIDHHLEKY
                     MSYHLGGWTEKQGEVLATNLLENESPLPTAILVGSDPMAIGVYRALKQKNIQIGEEIV
                     VASFDNIETIANLSPGLTSVQINAKSIGQAAVRMALERIDGIRDEPIILTYPARLIVR
                     ESFIPRTKRY"
     gene            complement(27664..28677)
                     /locus_tag="IXT20_000578"
     CDS             complement(27664..28677)
                     /locus_tag="IXT20_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAQ4859052.1"
                     /translation="MKKKILKFIGWFFGIILGIAFAIFLAFQLSPRPGAFLINHMFSN
                     TVQITDKKTFDQAEKNVAKKTDLTYESKYKENTYDIYYPKNSKGPVPVLFWVHGGGFV
                     GGDKSGVKEFATKLVSDANIAVVAMNYELAPDSEYPNQVLQVNELIKDLLAEEKDDKL
                     LDMEQLFFGGDSAGSQIALQYAAIQTNDAYAKQMKIKQLLPKDSLKGTISYCGPVDLK
                     LTAQQHSDDRFMKFFVKTVAWSLIGTKDWKTSEQLKEASVADHVSKEFPPTYITDGNA
                     YSFQEQGQALENRLTELKVPVQSLFYTDTKKEITHEYQFDYTLKESQNCYQQTLDFVN
                     KYK"
     gene            complement(28755..28943)
                     /locus_tag="IXT20_000579"
     CDS             complement(28755..28943)
                     /locus_tag="IXT20_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HAQ4859053.1"
                     /translation="MEEHQQVIDRIYEATRYLPLDRLCLSPQCGFASTEEENVLFEEQ
                     WKKFLLSKALQKKFGENH"
     gene            complement(29383..29511)
                     /locus_tag="IXT20_000580"
     CDS             complement(29383..29511)
                     /locus_tag="IXT20_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HAQ4859054.1"
                     /translation="MDQKEKMKELIKKKQGGGRSKQMETPKNDRKNMRKGPKIFNK"
     gene            complement(29514..30887)
                     /gene="rlmD"
                     /locus_tag="IXT20_000581"
     CDS             complement(29514..30887)
                     /gene="rlmD"
                     /locus_tag="IXT20_000581"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HAQ4859055.1"
                     /translation="MTEQQIVKKNERYTVEIVDLSYEGMGVAKIDGYPLFIENALPGE
                     EVEILVVKAGNKFGYGKVETFITQSPDRQPVENNALLRIGIAPLAHLKYEQQLVFKQK
                     QVEHVMAKIAKMPEVPVLPTVGMAEPFGYRNKAQIPVRRIDGQLATGFYKKNSHDLVP
                     IEDFYIQDPVIDQAIKVVRDILQRFQVRGYNEEKNEGQLRHIIIRRGHYSHEMMVVLV
                     TRKEKFFKSKEIAEAIHEELPEVVSVIQNINEEKTNVILGDKEKVLYGRDYIEDQLLG
                     KTYRISAKSFYQVNTQQAEYLYQTAIEFAELDKKDIVIDAYSGIGTIGLSLADRVDKV
                     YGMEVIPEAIEDAQFNALNNKIENVHYEVGKAEKVMKKWQEKGIKPSVIVVDPPRKGL
                     DHHFIESAIEMSPERIVYISCNPATFARDAKLFAEAGYAVKKVQPVDLFPQTHHIELV
                     ASFVKGE"
     gene            complement(30998..32053)
                     /locus_tag="IXT20_000582"
     CDS             complement(30998..32053)
                     /locus_tag="IXT20_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HAQ4859056.1"
                     /translation="MKKARVIYNPTSGKELLKKNLADILQALEEAGFEASAYATTPEP
                     DSAKNEACRVAELGFDLVVAAGGDGTINEVVNGIAPLEHRPKMAIIPAGTTNDYARAL
                     KIPRDNIKAAAEVIKKHQTVKMDIGLSDNSYFINIAAGGYLTELTYEVPSELKSIFGY
                     LAYLAKGAEMLPRVKPIKMRLKYDEGEYIGNASMFFLGLTNSVGGFEQIAPDAKLDDG
                     KFSLIIVKTANIFEILHIAALMLNGGRHVDDPRIIYTKTSSLFVETEDDINRPVMINL
                     DGEYGGEAPMHFQNLHQHIEFFANTDQIPDEAITGTDEEELEVVSKEFVKEVERLTDE
                     DIDGDGKIADETPNQKN"
     gene            complement(32063..33493)
                     /gene="gatB"
                     /locus_tag="IXT20_000583"
     CDS             complement(32063..33493)
                     /gene="gatB"
                     /locus_tag="IXT20_000583"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HAQ4859057.1"
                     /translation="MNFETVIGLEVHVELKTNSKIFSPAPAHFGAEPNSNTNVIDWGY
                     PGVLPVMNKAALEFGMKAALALNCEISKDTHFDRKNYFYPDNPKAYQISQFDQPIGHD
                     GWIEIEVEGKKKKIRIERVHLEEDAGKNMHGIGGYSYVDLNRQGTPLIEIVSEADMRS
                     PEEAYAYLEALRSIIQFTEVSDVKMEEGSMRCDANISLRPYGQEEFGTKAELKNLNSL
                     SFVKKGLAFEEKRQAKVLLSGGEIQQETRRFDETTNKTVLMRVKEGSSDYRYFPEPDI
                     PKFVIDDEWIQKVKASLPEMPASRRERYIREFGLPEYDAMVLTLTKEMSDFFEAVLEN
                     GADAKQASNWLMGEVSAYLNSEKLELAETKLTPENLAGMIRLIADGTISSKIAKKVFR
                     ELITNGGEAQEVVEKNGWVQLSDPNKLLPIINEILDNNQQSVDDFKNGKDRAVGFLVG
                     QIMKATKGQANPGVVNKLLKDELAKR"
     gene            complement(33490..34962)
                     /gene="gatA"
                     /locus_tag="IXT20_000584"
     CDS             complement(33490..34962)
                     /gene="gatA"
                     /locus_tag="IXT20_000584"
                     /EC_number="6.3.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HAQ4859058.1"
                     /translation="MTELHNRSLEELHHMLVSKEITVQDLTKTTFERIKETEPEVEAF
                     ITLNEEKALEQAKALDEKGIDESNVLAGIPIGIKDNIVTKDILTTAASKILYNFEPIY
                     DATVMDKIYQADMIPVGKLNMDEFAMGGSTETSYFKKTKNAWDPTKVPGGSSGGSAAA
                     VAAGQIPVSLGSDTGGSIRQPAAFNGIVGMKPTYGRVSRFGLIAFASSLDQIGPMTRT
                     VKDNALALTAISGYDEKDGTSSGVSVPDFAEGLTGDIKGMKIALPKEYLGEGVAPGVK
                     EAVLKAAETFRSLGAVVEEVSLPHSKYGVAVYYIIASSEASSNLQRFDGIRYGYRSEN
                     VKSLDDVYVNSRSEGFGEEVKRRIMLGTFSLSAGYYDAYFKKAGQVRTLIKRDFEKVF
                     ADYDLIIGPSSPTVAFGIGENINDPITMYMSDILTIPVNLAGLPGMSVPAGLSEGLPV
                     GLQIIGNYFDEKTMYQAAYAFEQATEFHKQQPAILGGKDE"
     gene            complement(34962..35267)
                     /gene="gatC"
                     /locus_tag="IXT20_000585"
     CDS             complement(34962..35267)
                     /gene="gatC"
                     /locus_tag="IXT20_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HAQ4859059.1"
                     /translation="MAISEEQVKHVAKLAKLAFADEELASFTDQLGKIIDMVEMLEEV
                     DTEGVPFTSNVVETVNVMRQDRAQAGWNRDELLKNVPETEDGFIKVPAIIDNGEAGA"
     gene            complement(35373..37409)
                     /gene="ligA"
                     /locus_tag="IXT20_000586"
     CDS             complement(35373..37409)
                     /gene="ligA"
                     /locus_tag="IXT20_000586"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HAQ4859060.1"
                     /translation="MEHEMTIDEAAKRAEELRTRLNQWSREYYVEDKPTVEDYVYDKE
                     YAELVAIEEQYPDLITSDSPTQRVGGKVLEGFEKVTHDIPLYSLNDVFSKEELIAFDQ
                     RVQKAVGRVVDYCCELKIDGLSVSLRYEDGNFVRGATRGDGTVGENITENLKTVRSVP
                     IKLKEPMNIEVRGECFMPKRSFVQLNQDREAEGKDIFANPRNAAAGSLRQLDSKITAK
                     RNLDTFLYTVADFGPMQAKTQYDALEELEKIGFHTNREKRLCHSIDEVWSYIEEYHDK
                     RVDLPYEIDGIVIKVNEFSLQDQLGFTIKAPRWAAAYKFPPEEVETLIENIEWTVGRT
                     GVVTPTAIMTPVRVAGTTVSRASLHNGDYIKLKDIRLKDTVLIYKAGDIIPEVSQVVL
                     DKRPKDSEEYQLPTHCPVCGSELVHLDEEVALRCINPKCPAQMKEGLNHFVSRNAMNI
                     DGLGPRVLEQMYDKKLVADVADLYKLTEEELLTLDKIKEKSANNILTAIDNSKDNSVE
                     RLIFGLGIRHVGAKAAKILAEHFGDLETLSKSDYESIIALDTIGDIIADSVVTYFSNE
                     EVHELMNELKQAGVNFEYKGLRNAQLQEVESPFKEKTVVLTGKLTRFTREEAKETIEN
                     LGGKVTGSVSKKTDIVVAGEDAGSKLTKAQELGIEVWTEDQMADALAKSRSVEE"
     gene            complement(37426..39660)
                     /gene="pcrA"
                     /locus_tag="IXT20_000587"
     CDS             complement(37426..39660)
                     /gene="pcrA"
                     /locus_tag="IXT20_000587"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HAQ4859061.1"
                     /translation="MSSKAELLNGMNPRQKEAVLHTDGPLLLMAGAGSGKTRVLTHRI
                     AYLIEEKEVNPWNILAITFTNKAAKEMKERVNAILASGGEDVWVSTFHSMCVRILRRD
                     VDFIGYNRNFTIIDSSEQLTLMKRILKELNIDPKKYDPRSILGTISQAKNSLQTPQDF
                     AKMQGSYYEEIAAKCYAAYQKELQYNQCMDFDDLIMNTIRLFEEHPDSLTYYQNKFHY
                     IHVDEYQDTNHAQYTLVNLLAGRFRNLCVVGDADQSIYGWRGADMQNILDFEKDYPDA
                     AVILLEQNYRSTKNILSAANQVIENNSNRKPKNLWTENKEGNKITYYRADNERDETRF
                     IVDRMQEEIRSNHRNYGDFAILYRTNAQSRVMEETLLKANIPYKMVGGHKFYDRKEIK
                     DILAYLNVLVNPQDSISFERIVNSPKRGIGPGSIEKLRSFASLHEWPLLEAAQNVDLA
                     NIGGKAGQQLGAFGEMIQEVTQMIPYLTVTELTKEVLDRSGYLEDLKIQNTLEAQARI
                     ENLEEFLTVTQEFDKQFEQQNEEDADAPEEKLTVFLNDLALVSDIDNLEEDASQVTLM
                     TLHAAKGLEFPVVFLIGLEEGVFPLSRALMEESELEEERRLAYVGITRAEEALYLTNA
                     FSRTLYGRTQYNRPSRFVEEIDQELLEIEGMRPTPKKTPVFAKKTAYSYKQPETAVVP
                     SKSATGGENNSWKPGDKVKHKKWGLGTVVRVSGTSKDLELDVAFPSQGVKRLLAAFAP
                     IEKA"
     gene            39815..41374
                     /locus_tag="IXT20_000588"
     CDS             39815..41374
                     /locus_tag="IXT20_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClC family H(+)/Cl(-) exchange transporter"
                     /protein_id="HAQ4859062.1"
                     /translation="MEKKGKIKKLDKTQIYFILKGIFVGLLTGIVVSLFRLSIEKLSD
                     LVRSIYEMSREQPIYLIGLAICCILAAFFVGYLVKNEPDIKGSGIPQVVGQLRGELSM
                     NWFSVLWKKFIGGVLSVGAGLFLGREGPSIQLGASIGQGAGQLFRSPSSEEKILISSG
                     ASSGLAAAFNAPIAGLLFVLEEIHHSFSPLVWLTSFASAITANFVSLYFFGLRPVLYL
                     GDLPSLPLKYYGSLVILGVLLGILGFIYQNVLLALPKFYNRLPLPAYFHGFVPFLLIL
                     PIGYLWPSTLGGGNQIVLAFGHASLPIIMIIGLFVLRFVFSMISYGGGLPGGIFLPIL
                     TLGALIGSFYGNIMVDFGMDPIYIKDFVVIAMAGYFTAIGKAPLTAIILVTEMVGSLN
                     HLMPLGLAALVAYIVNDLLGGNPIYESLLERLLGNTQTASLKGARTTFDFPVTAESSL
                     DGLMVRDFNWPKNMLLISIRRGNQEILTHGDTIMNVGDILVILTDEANLPRIKQEIEA
                     KSSITNLKQSF"
     gene            complement(41485..43416)
                     /locus_tag="IXT20_000589"
     CDS             complement(41485..43416)
                     /locus_tag="IXT20_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIA"
                     /protein_id="HAQ4859063.1"
                     /translation="MNIKDLMVKEAMIMDLQATDKKGAIDEMVQKMFDAGRISDIETY
                     KEGILAREAQTSTGLGDGIAMPHAKNSAVKEATVLFAKSQKGVDYEALDGQPTYLFFM
                     IAAPEGANDTHLQALAALSRLLIDADFVGKLKEANTPEEVQDLFQTAEEQKEAEEKAE
                     QETQAAEASAHPERKFVVAVTACPTGIAHTYMAEDALKKKAKEMGVEIKVETNGSEGI
                     KNRLTAEDIARAEGVIVAADKKVEMNRFDGKELINRPVSDGIRKPEELINLALSGTAP
                     VFHGDGKESSSEEGNADGTIGQRIYKDLMNGVSHMLPFVIGGGIAIALSFMIDQFIGV
                     PHDQLSNLGNYNQAASWFNQIGQAAFGFMLPVLAGFIASSIGDRPGLIVGFAAGALAN
                     TGGAGFLGALIGGFLAGYVIVFLRKLFKNLPKSLDGIKTILFYPVFGLLITGFLMLLV
                     NVPMKAINDALNGFLTSMSGSNAALLGALLAGMMAADLGGPINKAAYVFGTATLATTV
                     ATGGSVVMASVMAGGMVPPLAIFVATRLFKNKFSKTDQDAGLTNIVMGLSFVTEGSIP
                     FAAADPIRAIPSFIIGSALTGGLVGAFGIKLLAPHGGIFVVFLLSHPVMYLVFIAIGA
                     VVSGVIYGLLRKAPAVAEA"
     gene            complement(43434..44348)
                     /gene="pfkB"
                     /locus_tag="IXT20_000590"
     CDS             complement(43434..44348)
                     /gene="pfkB"
                     /locus_tag="IXT20_000590"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HAQ4859064.1"
                     /translation="MIYTVTLNPSIDYIVRVDGLKLGDLNRMKEDFKLPGGKGINVSR
                     ILKRIDSQSTALGFLGGFTGEFISKWLEKEQIQSLFTTVKEDTRINIKLKSDTETEIN
                     GLGPSISDEEIDELKQALGTVGQGDIVVLSGSAPASLRKGFYQELIDIIREKGADFVI
                     DTTGDDLKNALVKKPLLIKPNNHELAELYNTTFQSVEEIIPYGKKLLAEGAQNVLISM
                     AGDGALLFTEQGTYKTNVLVRPLKNSVGAGDSMIAGFIGAYSRTKDPIEAFKWGVACG
                     SATAFSDDLAARELIDELLPEVEITKID"
     gene            complement(44345..45097)
                     /locus_tag="IXT20_000591"
     CDS             complement(44345..45097)
                     /locus_tag="IXT20_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAQ4859065.1"
                     /translation="MLTEERRQKILDLLEQQKIVKSQDLVNLLNASESTIRRDLQELE
                     NSGFLERVHGGAKKEQLLNFEQNMSEKSLKNVHEKQQIAKSAAETINDEDVIYLDAGS
                     TTLEMIPFLQGKQITVVTNSVQHAANLVDLELATIILGGTIKLATNAVLGSNAIEQLK
                     NFHFNKAFMGMNGAHLEQGFTTPDPEEAAVKRLAMKNSEETFVLIDHTKFNQLSFTSV
                     APLEAATIITERCPLEFVNEFREKTTIKEAIQ"
     gene            complement(45256..45465)
                     /locus_tag="IXT20_000592"
     CDS             complement(45256..45465)
                     /locus_tag="IXT20_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4859066.1"
                     /translation="MDKQLYYRIMVPASLLSVCLFFVPNFPIRSVIGFLVMFGGMGLY
                     YYLLHKERKPASVYMKKTRSENKKR"
     gene            complement(45476..46804)
                     /locus_tag="IXT20_000593"
     CDS             complement(45476..46804)
                     /locus_tag="IXT20_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4859067.1"
                     /translation="MIGEDKLLMTIQLTDLNEEEKKQEISDWAILTRLLIQPTFIRSF
                     TQQADPYDLRKLQEKIMLASVRDESWLVVGNDENECSFRLEDNQLLIKNVLSISVFKE
                     KQFLIRDFIQKKMIAHGVFAYMRAYSEFIYHNTKQISQRLLFEKKDEIEHLPKMKQQN
                     GEVVVDCNQFPGYDLFYQGLCFTSCWEMYYSRYYHQIIPKPIFLDIQQVEKVKELENE
                     VICIQLYRDPFNWQKPNNQMFQSYFRDQLGFDHLAWDNGVGLLKPPFVEYIYTDHTIQ
                     SVQYQNAQMQPVPKKNASFFVTKSYDIQQGDYKERRVRGTLNAQAYFPWVDENRSRMM
                     CYKVIDPTVALDNGIEAYCYYIREYLEVEVTDEKYQEYLLSLRIYVPSESLSELPLKE
                     IKHRLSDVTFKRIKKKRRSIQFDVKKGEQHLRVQFLDYRELEQLITLQKI"
     gene            complement(47169..47756)
                     /locus_tag="IXT20_000594"
     CDS             complement(47169..47756)
                     /locus_tag="IXT20_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HAQ4859068.1"
                     /translation="MKVHQAEIIISAVAPKQYPDTQLPEIALAGRSNVGKSSFINTLI
                     DRKNLARTSGKPGKTQTLNFYLIENALHFVDVPGYGYAKVSKTERAKWGKMIETYLTT
                     RDQLRAVVSLVDLRHKPSQEDIQMYQFLKYYDIPVIVVATKADKIPRGKWNKHESEIK
                     KALDFDRNDTFILFSSVTKQGKEEAWAAIEQAIES"
     gene            complement(47839..49089)
                     /gene="clpX"
                     /locus_tag="IXT20_000595"
     CDS             complement(47839..49089)
                     /gene="clpX"
                     /locus_tag="IXT20_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HAQ4859069.1"
                     /translation="MYDNPSSNETVRCSFCGKTQEEVKKIVAGPGVYICNECIDLCKE
                     IIDEEFYEEAVREFTEVPKPLEILEVLNNYVIGQDRAKRSLAVAVYNHYKRVNQQAQE
                     NTDDVELQKSNICLIGPTGSGKTFLAQTLAKTLNVPFAIADATSLTEAGYVGEDVENI
                     LLKLLQSADYNVELAEKGIIYIDEIDKIARKSENVSITRDVSGEGVQQALLKILEGTV
                     ASVPPQGGRKHPHQEFIQIDTTNILFIVGGAFDGIETIVKNRMGEKTIGFGTQNQKLS
                     EDESVMQHIIPEDLLKFGLIPEFIGRLPVMAALEKLTTDDLVRILTEPKNALVKQYQK
                     LLSLDNTELEFEPEALRAIAKKAIERNTGARGLRSIIEEIMMDVMFDIPSNENIEKVI
                     ITKEAAELSGEPTVIYKDSEKKAG"
     gene            complement(49336..50625)
                     /locus_tag="IXT20_000596"
     CDS             complement(49336..50625)
                     /locus_tag="IXT20_000596"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HAQ4859070.1"
                     /translation="MTATFEKKGTNDGVLKFTIAQDEIQKGLTTAFNKVKKSLNVPGF
                     RKGKVSRQVFNRMYGEEALYEDALNAVLPEAYEAAVKEAGIDPVSQPKIDVESMEKGQ
                     DWVITAEVTVKPEVKLGQYKDLVVEKQDREVTDADVDARIKREQEAQAELVIKEDAAA
                     ENGDTVVIDFEGFVDGEAFDGGKGENYSLELGSGSFIPGFEDQLVGHKSGESLDVNVT
                     FPEDYQAEDLAGKEAVFKVTIHEVKAKEVPELDDEFAKDVDDEVSTLDELKEKYRKEL
                     VETKEKAADDAKDEAAIRMAVENAEIVELPHVMVHDEVHRSMDEFLNNMQRQGISPEM
                     YYQLTGSTEEDLHKQFEGEAEMRTRTNLVIEAIAAAENLQATEEEINAEIQELSETYN
                     MPIEQIKRVLTEDMLEHDVTMKKAIELITGTATEAKE"
     gene            complement(50779..51711)
                     /locus_tag="IXT20_000597"
     CDS             complement(50779..51711)
                     /locus_tag="IXT20_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4859071.1"
                     /translation="MKPIDFPEKYENLMRVAQQALANQQYQQAKELFQRAYELKESFE
                     ANSLLVFCLYELDEKKEALKQALLHEKQYLQNEEFAEFYFDLLISTQDFLYARKLIAS
                     TDFYEAFEQRVIEKIQFAEELSGQMERQKIKALHNKSEELPSLEPARQLASIDEIEQL
                     PYHEFIQTASKLIVSPEVHILARAKLLETLRQLNECHPVFYLTIEEKLVKVIPKDLPK
                     PQQQASYQQLCVFSDRYGNEDALLSSVLKEEFTLQSAIVYPVYDTYIEDPKRWFQLTV
                     EACTGKTMYEGTEDEKQDFLKKREKILQQMIFFH"
     gene            complement(51868..52527)
                     /locus_tag="IXT20_000598"
     CDS             complement(51868..52527)
                     /locus_tag="IXT20_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HAQ4859072.1"
                     /translation="MSELSNENKEQEKDKQQQPAVGPDSNFSQKVLSSFEREPLTPEE
                     IKKKQRKWKKYTDTRNEPPKANDFPKYFYAGFFIRMFAFLVDLLCIGAITRLTLGIAK
                     NLGWLNFSDSYLSLYGFTALLIYLAYFILLTKFNHGQTIGKMIFGIRVISFDEEELSW
                     STVLVREGACRFILKFPLLMLGYLPAVFNQKKQHLGDFFTNTSVVTINLIKAFNQEVN
                     S"
     gene            complement(52530..53555)
                     /gene="sppA"
                     /locus_tag="IXT20_000599"
     CDS             complement(52530..53555)
                     /gene="sppA"
                     /locus_tag="IXT20_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HAQ4859073.1"
                     /translation="MNKRRWAAVLIAAGLFVVSLVSASLTKPQENDQIEGLNSWLYGD
                     EELAPNVLEEGDSTQKIVKLSVDGTIAAGGSSGLFASEGYNHKNFMEQLKAIEEDPTV
                     KGIFLEVNSPGGGVYESAEIAKKLDTIRKEHDIPMYVSMKNMAASGGYYISAQADKIF
                     ATEETVTGSIGVIMSGLNYSGLLEKLGVEDTTVKSGALKDMGSGTRPETKEEEAVLQA
                     YIDSAYSRFVAIVSEGRNKSEEEVKKIADGRIYDGVQAKEVGLVDELGFPEDALKAIR
                     KEQQLEDAELVEYSSTPSGFASTWLGSKLAEFQGLKPSETSQILSVLENLGTAESPKA
                     MYYYGGE"
     gene            complement(53568..53858)
                     /locus_tag="IXT20_000600"
     CDS             complement(53568..53858)
                     /locus_tag="IXT20_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4859074.1"
                     /translation="MDSKIEIMTLGMLKKQLSKFEASAGVSDDTKIFLDTGWDSIQEI
                     APDALEVAQAREFTVEDELTKESFSGYAREEKAERFDTSEQSETVIVIKNLY"
     gene            54267..56072
                     /gene="glmS"
                     /locus_tag="IXT20_000601"
     CDS             54267..56072
                     /gene="glmS"
                     /locus_tag="IXT20_000601"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HAQ4859075.1"
                     /translation="MCGIVGMIGLKNVTPGLIDGLEKLEYRGYDSAGIFVSDGTTDYL
                     VKAQGRIQNLKNKINIDTTGNIGIGHTRWATHGQPSEENAHPHTSQSGRFVLVHNGVI
                     ENFEELKIAYLANDHFIGETDTEIIAHLIETFAKDTTTQEAFLKALRVIKGSYAFALI
                     DRTAPDVIYVAKNKSPLLIGLGDGFNVIASDAMAMLAHTKEFVEIEDEEMVTVTSEKV
                     IIQNFAGDIVERSSFEAQVDASDIEKGTYPFYMLKEIDEQPIIMRRIAQKYVTEDNRV
                     SLDQKLVDTLSDSDRIYIVACGTSYHAGLAGKQTLEKLTQIPVEVHLASEFGYNTPLL
                     SKKPFFIFLSQSGETADSRQVLVKINRLGYPSLTITNVAGSTLSREASFTLLLHAGPE
                     IAVASTKAYTAQIAVLTLLAKAIGDKKEFETSLVFDITHELSLVANAMESVIAQKDYL
                     EELAEVYLSDTRNAFYIGRGADYNVSLEAALKLKEISYIQAEGFAAGELKHGTIALIE
                     EGTPVIGIISEEVTGAHTRGNLKEVESRGAKTLVIVSEGLEKESDQLVLPTVHPYLTT
                     LTTVVPTQLLAYYATLLRGFDVDKPRNLAKSVTVE"
     gene            complement(56144..57499)
                     /locus_tag="IXT20_000602"
     CDS             complement(56144..57499)
                     /locus_tag="IXT20_000602"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HAQ4859076.1"
                     /translation="MGKYFGTDGVRGEANKELTPELAFKLGRCGGYVLSQHEEEGKRP
                     RVLVGRDTRISGQMLEEALISGLLSVGIEVFQLGVISTPGVAYLTRLQKASAGVMISA
                     SHNPAQDNGIKFFGADGFKLVDDQELEIEALLDAEVDELPRPSAEGLGTVEEFPEGLL
                     KYSQFLVQTINGDLSGLTICVDAANGATSTSVNRLFADLETDFYTMGTNPDGLNINDG
                     VGSTHPERLAEMVVEKGADAGLAFDGDGDRIIAVDELGNIVDGDKIMFICAKYLAEKN
                     RLKKDTIVTTVMSNLGFHKAVEGIGLKDVVTQVGDRYVVEEMRKNDYNFGGEQSGHMI
                     FLDFNTTGDGMLSGIQLLNIMKQTGKKLSELAAEVTIYPQKLVNIRVSDKNGAMDVPA
                     IKKVIEEAEAEMGSEGRILVRPSGTEPLLRVMAEAPTDEKVNYYVDKIAAVVKEEIGL
                     D"
     gene            complement(57523..58692)
                     /locus_tag="IXT20_000603"
     CDS             complement(57523..58692)
                     /locus_tag="IXT20_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4859077.1"
                     /translation="MISKEKRTSILYSFIALLFSIALYFNANGQSVPNTLSGNEAYNQ
                     TVTGVPIKISYDSKRYYIHGYENAVTVKLSSANRVQLNAEANEDTRMFRVTADITKLG
                     EGTHEVPLKIQNLSSAVTAKIEPSTITVTVEKKVIKDFDVETLIPSTFTPSGYELDDT
                     SVSPKKVSITTGDKTLAEIKRVVADVDASMVTDDGINSEVPIQALNAAGEVLSIVSDP
                     VQVTVRADSIKPSKSVRLYGIQQGTPAAGVESYDFSFSKLEAEVSGSSDLLASIGDSI
                     AVPINVSGINHRTTRKIEIPVEEGLSIKPESVSVEITPVLQTSSSSSASTSSRTQENT
                     STSRSISSADSGTEPSTTTISSSSESGTSSSTTSTEETSETSESTQMSRTNSSEN"
     gene            complement(58689..59576)
                     /locus_tag="IXT20_000604"
     CDS             complement(58689..59576)
                     /locus_tag="IXT20_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00159 family protein"
                     /protein_id="HAQ4859078.1"
                     /translation="MSFQIGELFNLDYWQQMFSGDLFSLNFLVNILDILVVWYLVYKL
                     IQLLRGTKAIQLLKGVAIFIVIRIVAEIIGLHTLSWLMNQVITYGVIAAIVIFQPEIR
                     RGLEHLGRSSLFKQSKSEQQEDEKMILSFDKAIQYMSKRKIGALITIERHTGLDEYIE
                     TGIALDADITGELLINIFIPNTPLHDGAVIVKEGKIAVASAYLPLSESMLIPKEFGTR
                     HRAAVGISEVSDAITIVVSEETGDVSITLDNELMAGLSQQEYLAILRRELIAEEKTKN
                     KKNVIQSFLDGMTKGGDRK"
     gene            complement(59765..63457)
                     /gene="nifJ"
                     /locus_tag="IXT20_000605"
     CDS             complement(59765..63457)
                     /gene="nifJ"
                     /locus_tag="IXT20_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HAQ4859079.1"
                     /translation="MRKMKTMDGNTAAAYISYAFTEIAAIYPITPSSTMAELVDQWSA
                     AGKENVFGQPVKVVEMQSEAGAAGVVHGSLKTGALTTTYTASQGLLLMIPNMYKIAGE
                     LLPSVFHVASRAVTTNALNIFGDHGDVMAARQTGFAMLAESSVQEVMDLAPVAHLASI
                     EGEVPFLNFFDGFRTSHEIQKIEVLEYEELAPLINQEKLASFRKRGMNPNHPTVSGTN
                     QNPDIHFQQRETINPYYDKLPEIVQKYMKEINRLRGTSYDLVTYYGADDAEEVIVSMG
                     SVAQTIEQTIDYLNANGRKTGFLNIHLYRPFPVETFVQKLPHTVKTIAVLDRSKEPGA
                     GGEPLLLDVQSALYDSELRPAVIGGRYGLGSKDVTPDQISAVFDELKKDPSIRKKRFT
                     IGIVDDVTYQSLEPKESLDLTEPQTFQAKFWGFGSDGTVGANKSAIKIIGDHTDKYAQ
                     GYFYYDSKKSGGLTVSHLRFGDTPIRSAYLVEHADLVACHTPAYLHSYDLVKGLKPGG
                     IFLLNTLWSDEQLETHLPLKLKRYLAENNIRFYTINAMRLAQEVGLGRRINTAMETAF
                     FKLADIIPFDEVLPLLKEEALKSYGHKSMAIVEKNIQAIDRTTELLHQVAIPDHWRTL
                     EMPEKINKRNTGYIQEILEPVNAQEGNQLSVGTLVANGMTTGEMPLGTASMEKRGVAL
                     EVPEWISDRCTMCNECAFVCPHAAIRPFLADEEEMEEAPEGFIVREMRGADGVKYRIQ
                     VSVEDCTGCGLCVEACPAKEKALVMKPYEEQKEQAVNWAFAMTLRQKANPVKGKNTVL
                     STQFNQPLLEFSGACSGCGETPYVKLLTQMFGDRMMIANATGCSSIWGGASPASPYTT
                     NECGQGPAWSNSLLEDNAEFGYGMLLAAQTRREALASSIKEVLPKVSEELRELMEDWL
                     VHMFESEGTQQRAAKLQAAIESERSIFPELEDIYKRKDLFVKNSQWMIGGDGWAYDIG
                     FGGIDHVLASGADVNILVLDNEVYSNTGGQTSKATPASAIAKFSASGKYASKKDLGMM
                     AMTYGNVYVAQIASGANQMQTIKAFEEAEKYPGPSLIIAYTPCITHGLLGGMKESLKE
                     AKEAVSSGYWSLYRYNPTMVEKNKNPMTLDYKKPDFSAMPDFMRKQVRFSSLDNANPN
                     LAKALFEKTVHDAKTRFYNYAALTGELEKIKKRLEPEESIDVPVKTRKEKTTDLEAEE
                     RRAARRAARAERRKKMEQATKEKKEK"
     gene            63656..64177
                     /locus_tag="IXT20_000606"
     CDS             63656..64177
                     /locus_tag="IXT20_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HAQ4859080.1"
                     /translation="MDISVIDATKTNTQKGILYGSSNAPKKMVEFINLACPYCRQWFE
                     ESYELLEEAVQSGQLQRVIKLFDKEKESLQRGNVMHRYLTISDGQKAIKEIKQIFDTQ
                     DEWKHLSLQEVADFAVDKLKLTEQKDEQLSQAVINEAEQAHIRFVPTVILGKEIFDES
                     ISIEELKELIQTK"
BASE COUNT        17833 a        13923 c        11462 g        20975 t
ORIGIN      
        1 caacctctta ttcacggtgt ttcagaagta gcttctcgga aataagccga aatttcacaa
       61 aaatttgaga aactattttt aggaaacttc ttcttatttc ttaaagctga ccacttctat
      121 cacaacctct ttttgactat gcattcacat cgatcacttt agaatcatca ttgatgacat
      181 cgcgatctaa atctttgatg cgtgcacttg tcacgatacc agctaccatg ctaccgctga
      241 cgtttactgc tgtacgtgcc atatcgatta atggttcaac agagataact aacccaacga
      301 ttgctactgg caagttcatt gcccctaaaa cgatcagtga agcaaatgtt gcgcccccac
      361 cgactccagc tacaccaaaa gaactgattg ttacaattgc cacaagcatc aaaataaatt
      421 ccaaactaaa gacattcact ccagctgctg gtgcgacgat cgttgccaac atagctggat
      481 aaacgccagc acagccgttt tgtccaatag acaaaccaaa acttgctgag aaattagctg
      541 ttgcatcatc tactcctaaa gcttttgtct gagtctcgat attcaatggc aaagctccag
      601 cacttgaacg tgaagtaaac gcaaagctca ataccggaaa ggcttttttg aaatagttga
      661 ttggattgac ttttgcacca atcaaaatca gtgagtggac aatcaaaaca gcaagcaatg
      721 ctacgtaaga agcaacgaca aatttaccta aattgacgat agctgcaaaa tcacttgttg
      781 ccaaaacgtt tgtcatcaaa gcaaatactc cataaggtgt caaacgcaaa acaagcgtca
      841 cgatccgcat aatgatttta tacagactgt cgactaaatc tgaaaagaat gctgcttctt
      901 ttggcgcttt tcttttcacg cctagataag cgattccgac aaatgccgcg aaaataacga
      961 ctccaatcgt acttgttgca cgactacctg ctaaatcagc aaaaacgttt ttagggataa
     1021 aggataaaat ctgctgtgga atcgataagt tttcaactgt ttcctgacgt gttgccagtt
     1081 cagagattcg tgctgtttct gcagctcctt gtgtgaattt agctccttgg agtccgaaca
     1141 gcattgttga accaattccg atcaatgctg caatagctgt tgttccaagt aaagaagtca
     1201 atacagtcgc actaattttc cctagctttt cagaaacttt catttttgtg aatgcaccaa
     1261 tgatcgaaat aaacactaaa ggcataacta gcatttgcaa gaatgataca tatccttgac
     1321 ctacaatccc gatccattcc attgattgag taacgacttt gtcgtctaca ccaaagatca
     1381 attgaagcag accgccaaag acgataccaa ctcctaatgc tgtaaagacg cgtgttgaaa
     1441 attttgtatg gcgcttttgc atacgataga acacatataa taatgccgca aaaactagaa
     1501 ccactaaaac tgtgatgagt gttgtcattt ttctcctcct catattatta atgacaagtc
     1561 catcgaagga agtaaaggtt cccgagaatg attcttcaac catcctcctg aaaaaatttt
     1621 tttcagtgac ttttatattt tagacgattt ttatcgaaat gacattcttt ttgcttacaa
     1681 ttagtaataa aaaaacatac ctgtgatagc tttaatctat cgcaggtatg tttttactgt
     1741 ctattcttgt ttttctgatt gaaaattcaa ttttacccca ggctgatcta aggcgatctc
     1801 tgtcacctca taagacattc ggcgtacgat atcgaataac ggtgctacta gctcgtccac
     1861 ttcttcttgt gtgacatcat tttgaccttc aattttccga ttgaggatat gattgatctg
     1921 actcacactt ccagataaaa catatgtatc aaacactaaa cggaactcta agcgtgctcc
     1981 tacgatgcta tgttctttcg gatagttttc gatcgtttct tttaaaggcg agaagcctac
     2041 tttcaaatct gtttctactt cttggttcgg caatctcata tcataatgaa atgcttctac
     2101 aacttctttt tgtcttctga tttccatata attttatcac tcctttatga aaattataac
     2161 atatctcctt tgaaagggga tactactttt taatgatgct ctgcaaggta aggatttcat
     2221 tcagatttcc taccacataa tctgccggga tctcacccag aaaatgatcg atgtcgtaaa
     2281 gacaagtagc gactcctgca tttttccctg cttcgatatc taaacgacga tcgccgatca
     2341 tcatcgtgtc tgttctttct aaatgaaaag tatcgatcaa ataatttaaa gatgctggat
     2401 caggtttacg aggaaagtcc tgatcgatcc ctactacttc ctcaatcaga tgtgccaatc
     2461 gatgttcttt taacaggccc catgttgatt ctgtcaaacg gtgggtcagg atgaaatgac
     2521 gaccgccgtt gtcttttagc atttccagta cttgtttcgt ttctggaaat ggacgggaaa
     2581 cttccgtact ttctttttca tatcggtgaa ataattcatt aaactctctg gcattcaagt
     2641 ttgattcatt gatcaaataa ctagtagaat actttttcat tttgaagtag atttcttttt
     2701 tgtccatact gataccaaaa tcttttagtg cttgccatgc accatctacc attgcgggat
     2761 acgtatcaaa caatgtacca tcaaagtccc atatataatt tttcatgttc cttttgttcc
     2821 attcctttgc ttcttttttc tatctctcac ttttagataa aggtgttctt taaagtattt
     2881 ttttgtccag aattgtcaaa tcatctgcta atgtatactg gattggttgt gcatttggga
     2941 tatcgatcgt atccatttga tcttcaggaa catcttctaa atattttgtc agtgctcgga
     3001 tgctattacc atgacccaca actagtaaat cttgccgatc ttttagtttt ggtgctagat
     3061 gatcttgcca taagggaatg atccgattca ctgtcatttc cagactttct ccgtatggga
     3121 tcaaatgcgg atccaagtga tcatatctgc ggtcaaatct attttctgcg gttaacggcg
     3181 gaacttccgt atatcctctg cgccatgttt ttacttgatc ttttccgtat atttctttca
     3241 tcttatcttt gtttttaccc acaagatccc cataatgccg ttcatttaga cgccatgttt
     3301 ttattatagg aagccataac tgacccattt cttctagtgc gacttgtccg gtaagaatcg
     3361 cacgtttcaa aacagaggta taaacggcat cgaattgaag tccggcttca agcatttttt
     3421 gaccggcaac tgctgcttgt tcttttcctt tttctgttaa ggcgacatct agccatcctg
     3481 tccagtaatt ttcaaaattt gcttcgcttt cgccatgacg taataatact aatttcattc
     3541 ttttctccct taaacattcg tttcgaattt tataccacat gttttgcatg tcacacgtat
     3601 tcgtcctttt cctttaggtg cacgctgctt ttgatgacaa ttcgggcact caaaaaaagt
     3661 ataacttttt cgatccattt tgacttgctt tttgtttttt atctgatgat atttttcttt
     3721 gactctatca agatatttca aataacgttg attctcattt agtctggggt agattttttt
     3781 tgataggaaa cgaaatatca tccatccgaa aaagacgatg aacaacaatt gtccgattct
     3841 atagggcaac caacgactta gcaaactaaa gacaagcgca gcgatcaata gaccgcgatt
     3901 caactgatca aatttcgcat atcttccctg cataagtttg tgatagttct gctgaaatcg
     3961 tattagcctt tctaaccatg tttgcatcat attatctcct ccatcatcta atatttatta
     4021 tgacaaaaaa atccaaaaag aaaaagcctt aattggcctt ttccattgca acttggtgaa
     4081 ggacattccg atttctggta aagtcaatca caatcacttt ctttttatct tctctgttga
     4141 ctactcgaaa tgtgtccgtc aatggaagaa acagcttctt agatggtagc tgttgcatac
     4201 gtataaaacg atggatcatc gtaatcaatt gaggaaattg aatagacgtg tgaagcagac
     4261 ctggatattt tttcaacagc tgtgtcgttt tgatcagatt ccgtttttta ctgatcactt
     4321 ttaaaaagct ttttagaatc ttttgctgac gtctaaaaat agaaaaagaa ttttcgtcat
     4381 ctatttgata acgcatttgt ttttgcagtt ctgctgcagt cagatggatc ttcccatcgt
     4441 ttgtatcgat cattaaacct tctggaaaca gttcaaccac tgctcgatcc caagattcaa
     4501 gagaaagctc gatataatct tttactggta attccaattc taccttaaaa aagtgcaaaa
     4561 cagccgacag cccttctgct tgatagattt ctcctaatgt tagcccctct agatggaact
     4621 ctggtaaaaa atgcagcaat gttttatttt cctgcaattg aacatataga aatgtagatc
     4681 gctcaatcag agtatctttt aaaacaacta aaaaattcat ttttctcccc cttttttcat
     4741 catttttaaa atagcatatt ttgaaaagaa agtctgatgc ttagagtaga atttcataaa
     4801 aatttactat ttcataagta ttcacgagaa aaatacaaac aaaaaagcgg aaataacgct
     4861 catccgcctt cttgtttgtg tttatttcct cttcaaacgc ttgtctatga ttcagacttt
     4921 tctctatcgt tattttcatc taaaagttct tgcattccga taatttctcg atattgatga
     4981 atcttagtga taccaatgac tttgtctaca tcaatactaa aacaaatacc attacctgga
     5041 atatcgatgt tgatgccttg accaatcgca tccattactt tattcgtcaa actatcgggt
     5101 actaatgtca acacaatatc tttttctggc gaaatgtcca aacctaaaaa tttccctgaa
     5161 tcatgtacac cagaacctct tccatgtaag atcgttccgc ctgctgcacc tgcttctttt
     5221 gaaaagtcga taacatcttc gcccattcct ccgtcaacga tcgtcacaat catgtgaaga
     5281 ttcaatgcta caagatcttt catttgcttt cccccaattt ttgcaatctt tctttttcga
     5341 ttctgtcttt ttcatctttt cgtttataaa tatatcctag tactaaagtg gataatattg
     5401 gtgtcaatgc aatcattgca accacaccaa acccgtcaat gatcggatct cttccttcaa
     5461 tttgtgcagc gattgccacg ctcattgata aaataaatgt cgaacacatc gggcccgttg
     5521 ctactccacc gttatcaaaa gccattgcta aaaagatctt gtccactttt cgtcctaaaa
     5581 taaatgctag cccgtatcct gggatcaaga aataataaag agagaatccg acgagcaaac
     5641 gccacataga caatcctact gctgcgccga tcccaattga aattaccatc agcatgattt
     5701 tcgatcggat tctcccctcg ctcaattctt caacctgttt gatcattaca tgaatcgccg
     5761 gctcagcaaa ccctactaag aaccccatta aaaagccaag gggaaccagt atataattgt
     5821 aaggcaatcc cgcaatagca ctgcctaaat gctgtcctac cggtacataa gccatattga
     5881 ctccgtgcag gaaaaggatc aacccaatag tcgttatcaa gaaacctttc ataatgtcaa
     5941 agaaacgctt tttgcgcata cgaactaatg atattcatta aaacaaatag gattacgata
     6001 ggtgccactg ccaaaatgac ttccatggta acgatttcga ttccatctat cacttgctgc
     6061 cagaaactca ttggaagatc acccccaaaa tcatcacggc aagaatcggc cctaacgaag
     6121 caataccgac cattccaaaa ctgtcatttt catttttctt ttgtttgatc atactagtca
     6181 tccctccggc aagggccaag ataaatggaa cagttactgg accagtagtc actccccccg
     6241 aatcaaaagc aatcggcata aattcggaac tggtgaaaaa tgaggcgatc aaaacgccta
     6301 tgtagcctat agccatcaat tgataatagg atagcttgaa tacgacccga agcagcgcaa
     6361 aggctagaaa tactccagtg ccgacgctga cgatcccaat cagcaacact ctgccaatct
     6421 ctccgtcaga tatatcatat acttgttgtc ccaaaacttg cacggaaggt tcagcaatcg
     6481 tgatcacgat cccgatagca aatcccaaac tgaccaaaac ggtcaaactt ttctttttga
     6541 tcatatattt ccctactaga tctccgactt ccatcataga ataatctgct ccaaacaaaa
     6601 agagcgtcat tccgatcatc atgattgccg caccaaccag aaatgaagcg aggtcttctc
     6661 cttctaacgg tgcaaaaaca aacgttaaaa tgacaatcag gacagtcatc ggcaatatag
     6721 cagcaatcac ttctttaaaa ttttttagca tgtatcctcc tcctttactc tttaagttta
     6781 ccttaaaatg aaagagtttt cgagcacaaa cttaaaacgt tatcatttaa tttttctcaa
     6841 cataaaataa cacacgtccc aaaagtatct ttttacttgg aaaaatgact ttgaaggaac
     6901 gcgcttattg tgttttttta ttttacagag ccgatcattc cttgaacgaa ttgcttttgc
     6961 aaaacaaaaa atacgatggc agttggtaaa gtagcgatga ctaatccagc catgatcatt
     7021 ccatagtctg gtgtatagga agcacccata gcagataaaa caagcggaac cgtccgttta
     7081 tcaggagatt gcaaagctac taagggccat aaataattgt tccagctact catgaatgtg
     7141 atgattgcag ccgcggcata ggtattttta gccgtcggca tatacactct aaaaaaaata
     7201 cttagttctt ttagaccgtc taatcgagcg gcttctacta gatcttttgg aaaagattta
     7261 acattttgtc gaaagaagaa gatcaaaaaa gcagtgctaa cagatggcag gatgacaacc
     7321 aagaaactgt tgattcccaa aaccgttccg ttcaattgtg aaaacattcg atataaaggg
     7381 atcatcaatg ccgcaaaagg aaccatcatc gatagcagta gaatattgaa aattaggtct
     7441 tttcgtttac tttgataaat ctcaaatcca tatcctgcca ttgacgaaat cagcaatgca
     7501 aacacagtgg tcaccaaagc gatcatcgct gaattccaaa gggaacgagt aaaattcagc
     7561 tcattggaaa acaaattttg aaaattgatg atcaagttac tgccaatctt tatttttccc
     7621 gatgtgatat ccacgggtgt attcgtcatt cccaaaacca tccagacaaa agggaaaatg
     7681 gaaataaatg ataggatgct taatgctgta tactttaata ctgacccaat tgtcgaacga
     7741 agttctaatg cttgtttttt gacgttgggg cgttcgattg tttctttttc taccatattt
     7801 ttcatcgact ttctcctcct actttcgttt ggattatcga caatatcaca ataaaggaaa
     7861 caataacaaa ggaaactgct gccgcatatc caaaattagg cgtgaattta taactcaaat
     7921 tatagatgta ttgagatagg gtggtcgttg catttgctgg tcccccgcca gtaatatttt
     7981 gtacttcatc aaaaagctgc aatgtcccga tcgtcgaagt aatggaagta aacaaaatga
     8041 ttggttttaa atttgggatc gtcactttga taaactgctg ccatttagaa gcaccgtcaa
     8101 tagatgctgc ttcataaatc tccgaatcaa tattttgcat gcctgaaagg aaaaaaatca
     8161 tattgtatcc agtccaacgc caagtgatcg aaagaatgat cagaactctc gcccaaattg
     8221 gatgagtcaa ccagccaatc ggtgtgtcaa tcataccaat gcccaacaac aagttgttta
     8281 caaggccaga ttgagaaaat agacttttca tgatcagaga ataactgacc atcgaagtga
     8341 tacatggaag aaagatagcg gttcgaaaca accctttata tttcaacttt ttatcattta
     8401 aaaggttgga aataaaaagt gctagaaaca acatgatcgg tacttgtaca agtaaataca
     8461 ggaatgtatt aaaaaaagct tttttaaaag ttgcatctgt caacatccgc tgataattac
     8521 ctaacccgtt aaatgtcata ttgacagggg gaccactttg aaaagacgtg atcaaagcca
     8581 aaaccatcgg aataaaaacc atgattgata tcaaaatgac tggcaagatt aaaaaagcat
     8641 gcccgttttt gaataaatct ttctgcttca ttcttttccc tccaattcta tcattgatta
     8701 tctacatgaa aagacaagaa agacaatatc tgtaaggcag acgtgatctt ttttgtcttt
     8761 tcgtttgtct gatgaaaaag cgaagaaatt cactttttta ccaccgatca attagccaat
     8821 tgtgcttcta cttgtttttg tgtatctgct agtaccttgt ctgtctcttc tccattgatg
     8881 attcgatgaa gagcttcagc tacgactgct tcaattgcat aggtctcatg tccgtaattc
     8941 acttctggta tttcagatgt ccatgttgaa aaatcgtcaa agatcttttg accactgtaa
     9001 aattcagaag cttcttgata agcttcttgt tcattcgcgc taagcatcgt agatactagt
     9061 ccgatttctt tcgcaagagt gcccatcagt tcttcattcg aagcaaacgt tgcttctagg
     9121 aaatctttcg ctgaatcttc tccagctact cctttgatca cataccagcc agcgccgcca
     9181 aggttagaag cttgcgcttt ttgcagattt tctggaagtg caggtattgg tgcaatcttc
     9241 cattttccag attggttttc tgctccttgg atcgttgaag aataccaagc gccagttggg
     9301 ctagaagcta ctgctcctga ttgtatcgca gttactccag cattccagtc agaggtttgt
     9361 tcgactagat cttctttcaa aagagtggca aacaattcaa gaccatactt taaagatgca
     9421 ttgtcttgga tcgtgactgt ctccccatct tcagatgtat accattctcc tgcctgttgc
     9481 ataatcatcc ttactcgacc aaggtcagaa ggattgactt cagcgatttt ctttcccgtc
     9541 ttttctttga catctcgcgc tacttgaata tactcgtccc acttcagatt ctccatatcc
     9601 tcttctgtat aacctgcttc tgccatcaga tccgtacgat agaagtttgc tgttacgccc
     9661 gaatcgaaag gaacaccgta tattttatct cctactttgt ttaccgcaaa tttatacggt
     9721 gcaaaatgat cttcctttac aatagcagac aaattagcga atgcatctgg ataagaagtc
     9781 aaatagcctt gtatcctata atcttcaatc aagacgatat tgggcaatcc ttctgtattc
     9841 ccgctcgcta aagcggtgtt cagtttctga acgatatcat cctgagacat cgtaacaact
     9901 tctatctctg tttcttcgtt ctcataaact ttttttgctt cattcactgc tttgatattg
     9961 aatgtctcat cccacgccca gacagtgact ttttcttctt tgtctgctgt actactagca
    10021 cttccgcatc ctgttaagaa acctgatgct gccgctaaac aaacgactcc agcaattaac
    10081 tttctgatgt tcatttttat ccgctccttt tccatccatt agttgtttac gtttgctaca
    10141 ttcatcatac caaaatttga aaacggtttc tttattattc agatacttca aaaatttctc
    10201 ctaaaataat aaaaaaatac aaatcaacta tgacaaaagg aataatctta gcatctagac
    10261 caaagatcga aatggtgagg tttctttaaa ataaaaatat ccttgcattt tctcctagaa
    10321 ataagtattt ttaaattaca agactattct taaggcgggt aaaaaaatgc caatacgcgt
    10381 aattcttatc gatgatgaac agacgatttt agatggaatg aaacagttga tcaattggtc
    10441 tgagcacggg tttcaattaa tagggacttt ttccagtccg ctgaaagcgc ttgatttttc
    10501 tttggtacat gcaatcgata tcgtggtcac agatgtaatg atgcctgaat taaatggaat
    10561 cgaattatcg cggctattaa aacagcaaca acctgaaaca cagatcctta tcctctctag
    10621 ctatgatgaa tttgatatgg ttaaagattc ttttaaagaa ggcgtcgctg attatctttt
    10681 aaagcctcga ttgagtcctg atttttttct aaattcctta actgccgttt cacaaaaaat
    10741 caaaaaaaag cccaacaaaa gcccaatcac caatcgttat gagcagatct gcgagaatct
    10801 ttcacagcag attgctggac aaactcgtcc attgcttgac cctcttcttt ttgaacacac
    10861 cttgtttttc tgttatatac cgaaaaacgg ccattcagtt caaaacaagg ctctaaacaa
    10921 aaacagtcga tcacccattc attgatggat agcgacgatt cgtttactat ttatcctgtt
    10981 tctacctttt cggaagaatc cggctatgta atcaatactg attccaaaga tacacttagg
    11041 aagtttgttc aaaagtttcg tcaagaagtt tttccaggtg atttttttgt tgtttctgat
    11101 tcttttgcaa tccaagatat caatgaaata ttccaagaga tcagaaattg caccaaagga
    11161 caacgtttct atcataaggg aaagaacctt gcttatcagc atgaacttct tccgttatta
    11221 accaaccacg agcagcaagc aagtagttat ttaacaaaaa tcgttcataa agactacgct
    11281 ttagctgtca aagaacttga gaatttcttc acgacgatat tggaacagcc tattgatcca
    11341 gcttatttga aacatgaggc aatcaatcgt ttctatgcat tactcaacgc tttggctgac
    11401 gagcatgttg ggaatgaaga actttctttg ctcaagatcg cccttccttc tttattagcc
    11461 gatgctgagt atttcgaaga tttttctatt cttgtcaaag aaacacttga gaaactcacc
    11521 cacttttctg caaatgcaaa aaaagacaat ttcgatatct tgactttgat ctacgagttc
    11581 gtccaggaaa attatgagaa agaaatcaat ttacaacttt tatccgaaaa atatcatttt
    11641 tcatatagct atctctcagc catctttact gaaaaatttg gtatcaactt ttcaaaatat
    11701 ctaaaaaaag tccgtatcag taaagccaag ctctttttga cggaaacacg gcttactttg
    11761 acggaaatct gtgagaaagt cggctatacc gaactgggtt atttctccag agtttttaaa
    11821 gaagagacag gaatgacacc ttctcaatac cgtaaaggaa cttttgtaaa atgagaaaaa
    11881 aaatcaaaga gcatgaactc tttgccaaac ttctgttcat tttgttcatc ggtctttttc
    11941 tgcaagccat cgtcatttct ttgtttattt atcatcgatc aagggatgcc tatatccagc
    12001 tgttcaatca atcgaatgat gtggtactaa aaaagattca atctgaattt gaaagtttga
    12061 atgatacaat cgagaataca ttagccgctt ttgattctaa tccagctgtc aaaagctatt
    12121 tttccaatga tccagcccaa catatggaac agcttcagca actaagaaca atccaaaaaa
    12181 tgaatgactc tctttcaaaa atccatccaa tgatcgatta cgatgtattg atctttggag
    12241 aaaatggaag aacttttgtc ggaaacgaca tgctcactgc cgtatctgcc gattcttttt
    12301 ttcaatctgc gattgctcag agagtgaatg aacgtgctgc tgatacacaa atgctttttg
    12361 cacaccacgg attgactctt cgcgataaga aagcacccag cgtcttcttt gtcaaaaaac
    12421 tgaaaaatac attgaatcac gtttacggat atgctgtttt atcgatctca tccaagcaac
    12481 tagcgaatct ttttcaatct gtagtaaatc ctgagatatc gaagatctcc ttcatgaacg
    12541 aagaacagac gatcatcgca tcaaatgaag aaaatacaat tgggagccga tcaatgattt
    12601 tcaaaaaact aacagtaggt gaaacaacga tttcaaatgg acagcagttg acccgtcttc
    12661 ctctgtaccg ccaggattgt acattactca gtcaagtcga tcttcattct ttatctaacc
    12721 aaatgggcgt gatttttccg attattttgt ataatatcgt gacacttgct tttgctggct
    12781 cattggtatt tatgtatttg gatcgacaca caaaatcgat ctaccgcttg attgattcgc
    12841 taaaacggct agaggaagat ggcttaaaag ctactgttcc tgtacaagga agccatgaag
    12901 tccaattgct agggaatacg atcaatcaat tgctaagtaa tgctcagcat cattatgaac
    12961 agacgatcca agacgaaaag aagaaacgtt cgcttgaaat ccaaactatg caagcacaga
    13021 tacagcccca ttttatttat aatacgctga ctagcttgaa atttttgatt tggcagaaaa
    13081 aaacagatga agcagttcaa ggaatcgaca gtttcattac tttactaaga agtacgattg
    13141 ggaaaaaaga agaagtgatt ccagtcaagg aagaaatcaa aagcgtacaa agttacatcg
    13201 atcttttgag tatgcgatat ggcgaaagaa tctcggctaa aataatgatt cctgatgaac
    13261 tgcagtcatt atctatgccg aatatgatcc ttcaaccaat tatcgaaaac gcctatcttc
    13321 atgcttttca caataaaacg caaggattca tcacggtgta cggcaaaaca aaaaatcagc
    13381 ttcttttatt cgaagtgatt gataacggcg atggcttcga tactgaaaat accaaacaaa
    13441 aaaatgatca tttttcagga atcggcatca gccatgtaga tgagcggata cgcctaatgt
    13501 atggcgatgc atatggctta acgatccaat cagtgattgg gacaggaacg actgtaacga
    13561 tttgtctacc gcttcttcct aaaaatcaat cagaatatca aaaataaaga atagccaagc
    13621 gctgattttt ttattttaag ttaaataaat caatcagtat tcttgacttt catctacgcg
    13681 tctcttctac tgtcagcact ggataacagc ccaatatttg actttctcct ccgagcatct
    13741 tgatttcttg gagcgcattc tctactaatg tcattggacg gtccaacaac aaatcgatca
    13801 cgaaaaaata ttctcccaag ctagttttca atggtcggga ttcaatctta cttaagttga
    13861 tttcccgcca gccaaaagct gccagcattt tatgaagcat ccctggccga ttagcaggta
    13921 gtgtcaagat gacactcatt tttactggag ctgactgctg gctggtcatc tttcggtcac
    13981 cgacgatcca aaaacgtgtc tggttcaact cattatcttg gatattctca gctaagatct
    14041 ccaatccaac atgctgtgct gtttctaatg aagcaatcgc tgctgcgtct tctttcggat
    14101 gttctgctac atacattgct gcagcagttg ttgattctgt tgctactaaa ggaacattag
    14161 gataatgcgt ttctaaaaac tgttgtgatt gtgctagtgc ttgtggatgt gaaaggattt
    14221 ttgtaatctt tgttgctgga ttgcctaaaa gctgctgctt gataggaaga acgatttcac
    14281 ttttaacttc gacttctgga tgtttggaaa gaagatcgat tgtgtgatga acagctcctt
    14341 ccaatgaatt ctctacagga acgacagcca gatcgacttg tttgcgaaac aaagcgcgca
    14401 aacaaacagg gatggaagca tacgcagtca attgttctgt aggaaatagc tgacaagccg
    14461 cctgataagt gaatgaactt tctggcccta gatagctgac gttcatatgc ttttgatcct
    14521 ttctataatg gtatcgatga tcttttctgg tgtttgattg gtcgtgttga ttcgatgtgt
    14581 cgcactttct tcataccaag aaagtcgttg tgccaataat gcttttattt cttctcttgt
    14641 ttttccaaga gctaaaggtc gaatagacgt ttcatcttgt tggatacgat caactagaca
    14701 gtccgcttct gcctctaagt aaagaacaat cggctgtgtt tttaaaaaac gccgattttc
    14761 tgcactttgg acaatccctc caccagtcgc gataattttg tcttcatttg acagctttct
    14821 taaaatatct gactccactt ttcgaaagct tttttcaccg taatagtgaa aatattcggc
    14881 aatcgacaat tggatatgtt cctcaatctt tgtatctaaa tctacatact ctttttgtaa
    14941 acattgagct aaccctttac cgattgttgt ttttccagcg cccatgaaac caatcagcaa
    15001 aatgctagcc atgagcttct cctcctgaca caagttctgc taaatcgtca aaaaatgccg
    15061 gataagagat tgaaacagct tcagctcctt ctagttcgca aggtccatca gctatcaaag
    15121 cagctacttg cagcatcata ccgattcgat gatcgcctct gctggacact tttgcgccat
    15181 gcagaggtgt cggcccatgg atgatcagac cgtctgctgt cgcttttatc tttgctccca
    15241 ttttctgtaa ttcttctgcc accgcatcaa ttctgttggt ttcttttact tttaattctt
    15301 ctgcgtcttt gatgatcgtg attccttctg cttgagtagc cactaaagct agaatgggca
    15361 attcatcaat cagacgcgga atgatttctc caccaatcgt tgctttcttc aaatgactcg
    15421 tcttgacact taaatcagcc gattggttct gttcattgtg attcgtctgg gtaatcgctg
    15481 cgcccatctc ctccaacaca tctaagatac cagttcttgt tggattgacc ccgacatttt
    15541 ttaacaacag ttggctttcc ggaacaagaa gtcctgctgc tagaaagaaa gcagctgatg
    15601 aaatatcacc tggaacagtt atctgttgac cagttaattt ttgcggtccg gtcactatga
    15661 tcgttttatc ttccactgtg atccttccgc caaattgacg aatcatttct tccgtatggt
    15721 tacgggaacg ttctttttca acgattacgg atgtgccttc tgcttgtaat gccgcaaaca
    15781 gcaaagcaga tttgacttgc gcactggcta caggcatatt gtatcggata ggtgataaag
    15841 agcgggttcc tgtcacagtg attggtagat actggtcatt ttcttctcct tgcaagtcag
    15901 cgcccatctc gcgtaaaggt tccatcactc gccccattgg acgtttagac aacgaagcat
    15961 ccccgaacag agtagtcgta aaaggctgtc cagccaaaat acccatcaat aaacgtatgg
    16021 tcgtaccgga gtttcccatg tcaattgatt cggttggttg gacaaatgag tggctaccgc
    16081 gaccatgaat cttgatcacc tcttcctctt cttcgatctc gacacctaat tgttggaagg
    16141 catgtaaagt actcagacaa tcttctgcgc gcaaaaaatt ttgtatagtc gtcgttcctt
    16201 cagcaattgc tccaaacatg atgctgcgat gggaaattga tttatctgct ggtatcctaa
    16261 cagtccctct taatccatgt atttgttgta ataattgcat ttatccagcc tcctatttga
    16321 tccagcaatg ataactggta ttagacataa tgattctttt tgcttgtaac aaatcctgtt
    16381 ggtttttaaa actgatttgc agtattccta aaatatcttc cctggtctct tggatcttca
    16441 aattaatcaa agaaactccc gcttttccga tcagtccagt tacttctgca ataacgccag
    16501 gttcatctgg cacatcgata aataaatcaa aaaaggcagg aatcgcgcct tgcttatgga
    16561 caggcaactg gtctcgactt tcttttgccc gattgaaaaa agaaaagatt tccgattggt
    16621 tctcagtcat gatccattct gctatttgtg tcatttcttg ttgccaatct gccaatcgtt
    16681 ccaataatat ctgcttattg ctcaacaaaa tatctgtcca catgacagga tctgaggaag
    16741 cgatcctagt gatatctcga aagcctcctg ctgccagttg tttggctcgc ggatactctt
    16801 gattgaattg ttctgcctga ttgactaagc cagctgccag aatatgtgga aaatgactta
    16861 gcattcctgt tatacggtca tgttctcttg ctgtaagagt tacatatttt gctttggttc
    16921 cgcaaaatag ttcttgcagt ttttcaacat cttttttcgt cttttccaaa ggtgtaaaga
    16981 tgtaataggc attttcaaat agtttagcat ctgctgcctg tatacctgat ttatgagaac
    17041 cagccattgg atgtccaccg acaaaccgaa gatttttctg ctcagcggat gtcacgactt
    17101 gctgcttcgt actgctgaca tccgtaatga tgacgtgatc agacaagttg attctttcaa
    17161 ggacatcaag ataagctaag gttgtacgga cgggaacggc taataagata acgtctgctt
    17221 gttctgcacc tgtttcaaaa tcacttgcaa cgtaatcaac gatctgctgt tctactgctt
    17281 gctcacgggt ttttgactga ctatcccaac caactactat cagttctgga ttggtctgac
    17341 gaatacaaag tgccatagaa gcaccaatca gtccaagccc cacgataaaa acatgttgtg
    17401 ccatcttatt tgcccccttt ttagtaatgt ttcaagtagt cacggtactc cttcactgca
    17461 gctttcaact caacaagcga atcacttggg aattttgaca atagctctgt cgctaccaca
    17521 gtggctacaa cattttccgc aacgacgcta gctgcaggaa ctgctgtact atccgaacgt
    17581 tctacacttg ctttgtaagg ttcttttgta tcgatatcaa cactttgaag tggcttgtac
    17641 aatgtaggaa tgggtttcat gaccccgcga atgacaagtg gttcgccatt ggtcattccg
    17701 ccttcgaagc cgcctagatt attggtacgt cggaaatagc ctctttcttc ttcccatagg
    17761 atctcatcca tcacttctga gcctggtaat gaaccagaag tgaatccgac cccaaattct
    17821 acgcctttaa aggcattgat actaaccaca gcttgtgcga ttttggcatc tagcttcgtg
    17881 tcccattgta cgtagcttcc taatccagct ggaacgcctc caacgagcac ttctacaatt
    17941 cctccgatcg tatctccatt tttctttgtc tgatcgatca aatcttttat tctttgctca
    18001 actgctggat caacaactcg tacttctgac gcttcagaaa tttctttgat ttcttgaatc
    18061 gagatatttc caggtatttc tgcttggatt ccaccaagac aaatcacgtg tcctgctacg
    18121 atgatgtcaa gttcttccag aattttcttg gcaattgcgc cgattgctac acgcatggtc
    18181 gtctctcttg cagaggaccg ttcaagcaca ttccgaagat ctcgatgatg gtatttcata
    18241 ccgcctacca aatccgcgtg tcctggacgc ggacgtgcaa ctcgccgcag tgattggtct
    18301 ttttcaggca cttctgctac agacatgaca gttgtccaat ttttccaatc tttattttct
    18361 accactaaag tgacaggtga accaattgtt ttgccgtgac ggatgccaga cgtgatccga
    18421 acacgatctt tttcgatctt catccttcct cctctgccgt aaccagcttg tctgcgagcg
    18481 agttcgtgat tgatttcttc ttctgacaga ggtaatcctg ctggtagtcc ttcaataatg
    18541 gctgttaatt ctggtccatg ggattctccc gcagtcatat aacgcatctt acagtcctcc
    18601 taagtaatct ttgaatgtct ctgtgggaac aggaacgatt tttgcttgcc caatcttttc
    18661 tagcaagatg atattcaatt ggcttcctct tgctttctta tcatgggtta tcgcgtggaa
    18721 taattcatct ggatgactta gatcgtacgt aatcggcaga tggaattttt gaagtatcct
    18781 ttctaaagca gctgtcgatc cagttggaga caagccgatt ttttctgcat gagaagtgat
    18841 ggctaccatt ccaatagcta ctccttctcc atgactgact accccatatc ctgctgtatt
    18901 ttcaattgca tggccaattg tatgaccaaa atttaaaata agtcgatttc cttggtcaaa
    18961 gacatcttct tccactactt ttcgtttgat ttccaacgta gcagtaatga ttggaacagc
    19021 atgttccaga agatcttttt cgtctttcag ctcgttcagc tgtttccaaa gcgttgcatc
    19081 tgctatagct gctgatttga tgatttcagc gattccttct cgtaaacgac gtaccggcaa
    19141 agtttttaga acctcaggat caatcaatac accagctggc tggctaaatg ttccgacaag
    19201 gtttttcgct ttgtttgtat tgactgctgt cttcccgcca atactagaat ctacttgtgc
    19261 aagtagcgta gtcggaattt gaacgaaatg gatcccacgc atgaacgtcg aggccacgaa
    19321 tgctgctaga tcacccacca ttcctccacc aagtgcaagt atcccatcgc tgcgggtaaa
    19381 atcattatct gctaaaaaat cgtataactc agctgcttgt tctaaagatt tactggtctc
    19441 acccgccggt acgattgaaa ggactggttc aaaattagct tgtctcaatt ggaacatgat
    19501 tttttctgca tatagtggag caacgtttgt atcagtgatc acagctactt tttgaggtct
    19561 ccaaatcgtt tgaagccatg atccagcctg ttctagtgcg cctttttcaa tatgaatatc
    19621 gtatttttgg ttatcaacat gtacagtaag cattcgtttc acttctttct tttaaaaagc
    19681 tggaattaat cgagtgtctg aatcttttcc attgcttctt tcatgatcgc aacttctttc
    19741 atcatgcgtg agtaagtctt ttcattcaac gattggggac cgtctgacca agcatttgca
    19801 ggatcaggat ggatctctac gatcatgcca tctgctccgc ttgccacacc cgcacgagcc
    19861 attggtggta caagttccca aattcctgta ccatgacttg gatcaacaat cacagggaaa
    19921 tgagttaatt ttttcatcaa tgggacagca cttaaatcaa acgtattacg tgtcgctgtt
    19981 tcgtaagtcc gaatcccacg ttcgataaaa atgacaggag atttgtcttc gactgcaatg
    20041 tattctgcag cattaagcca ttcattgatc gtaccggaaa ttccacgttt caatccaaca
    20101 ggtttacctg ttttaccgac agcagatagc agtttaaaat tttgcatatt acgtgcgcca
    20161 atctgtagga tatcgctgta ctcagccacc atatcgatat gcccttcatc catcacttca
    20221 gtaatcactt tcattccaaa ttcatcagcc gcttgacgga tgtatttcaa tccttcttct
    20281 tctaaacctt ggaaagcata tggagaagta cgtggtttga aggcaccacc tcgcagaatt
    20341 tttgcgcccc cggctttggc cattcgcgca cattctctga tttggtctaa tccttcgata
    20401 gaacaaggac cagccatcgt cacgaaactg ccgtcaccaa tttttactcc gtctacatcc
    20461 acgactgtat tgccaggatg gaattctcga gaagtcaatt tgtatgtgtt taaaatacgc
    20521 acagcatttt ctactccttc atagcgtgta aatgctcctt cttgtaaact tcgcgtatca
    20581 ccttttagtc caatcactac tcgttctgtt ccttgatcga ttgagacttt gaatccttct
    20641 ttttcgatat gatcgacgac tttttttatt tgttcttccg ttgcttcttt tttcataatt
    20701 actatcattg tttttcccgc tttcttttgt ttacttatct atcttggctg attttctcta
    20761 ctttttcaag gtggttttac attaattcct actaaatgac cgtgtcctgt tgttctcatt
    20821 ttcgctcctt ctttcctact tatgacccag ataatcaaca aaaaaagatg tccctgtagg
    20881 aaattgcctc tacagtgaac atcttcaacg agccttaaaa agcgctgcaa aaatataaaa
    20941 tagccactat agatgaattt ctctacgtgg ctgtaagtcg tgtttaaaaa tctttagcca
    21001 tcatagatac aaacctggtt tgtttgtacc cacgaagtca tgaatacaaa cctatctaaa
    21061 atagctaaag taatacgtat tcagttttgt aagttttcta gatttcataa aaaatcgctc
    21121 caaactgatg taagaattgt acctagtata gacataaaaa aatcgactgt caatcatttt
    21181 tatgaaactt tttttaattt tctgatagtt aactttgttt ttcagaaaaa taacaattgg
    21241 ttttattgaa gaaaatagtc atgaacaaaa gtatctgaga acttttttac tctcttgttc
    21301 atgacatata gaataaagca gctttgtctt gtaaactccg tttttcaaat aataatctaa
    21361 agattgctac tgaataataa agccgatttc atggtcctct tcgctgctga ttcggtaagg
    21421 tgactcaaca tccgctcccc agctattgat acccccgact cctcttactt ttgcaaaaat
    21481 cgtaagaacc gttcgtcttt ctggcggtag ttcttctata tgtgttgcct gttccagttc
    21541 ttctgctttg aatggtaaag cggaaaaagc aaatggcgta tcctgctgtt caaatcgcaa
    21601 cgaaaaatct tcttgatctt tatccgtatt atccaacgtt tgatttcttg ttagttctac
    21661 ccatttcgtt tccatatgca tgccgcattc ttggggaaca aggtatgggg aaacggggag
    21721 tcctgccact ttataaatac cttcgacacc accagccatc cgatccggat atgtttcccc
    21781 tgataggcct tcatactcaa aaaattttgc tttcgttggc ataatgaacc ttaaacccaa
    21841 tgcgggtaat tccggtaatc ctttttgtcc ataataatgc atattgacaa gcattttacc
    21901 ctctccagcg atttggtagg aaattgtcac ttgggttttt ggtactgtgt tgatttccag
    21961 tgtaaaaatg atttcaaccg tttcagcgta ttcattactt gaatattgat tatttactgg
    22021 agcaatcggc aaatcgatct ttttattgtc caccagtact tctatttctt tgacttttgg
    22081 atacaatccg gctcctaacc acataccgga tttaaatcca aatccgctac ctctgtcatt
    22141 atcggtcgtt gctcgccaaa aaactggaag tgtttcccgg tatagccatt cttttccgtt
    22201 tttattgagc gattccagtc cgcctcttgt ataagaaaaa atataatgcg tattccccga
    22261 tttgacacct aatgtacagt cgccgaagac gattgcaact ttttccgtat ccatatgact
    22321 tttatttact gtatttttca taataatatc ttacctcctg catggcgggt ttttcttgtc
    22381 catctgcaaa aacgatccca ttccctgaga attcatagtc acttggttta tcgtcaaagt
    22441 caccgccata gcgcatcact tctctgcctg tgattggatc agtcacttgg atcgcctgat
    22501 ctttaaaatc ccatatatag cctccttggt acatttcata acgatcaagc aacgtcatgt
    22561 aagaatccat tccacctaac gaattcccca tgtcatgcat atattcacat aagatgaacg
    22621 gtttttccgc tttattttca aggtagtcaa tgatctcttc tggtggcgca tacatgcagc
    22681 tttttacatc tgaaatcgaa tcttcgaatg ctggatttgg aaaattccct tcataatgga
    22741 caagtcgatc ggggtctact tttttgaagt aatcgttcat ttgtcgaatg ttttcttccg
    22801 cataagattc atttccaaga gaccaaaaaa gaatacttgg atggtttttt aatgtttcga
    22861 aattcgtttt tgctcgatca agaacagccg cctgccactg aggtactgca cctggtacgt
    22921 tgtaagaagg ctcaaatgcg cccatttttt gccatgagcc atgggattcg aggttcgttt
    22981 ccgccatcac atggattcct gcttggtcgc ataaataata ccaagtgatc tggttggggt
    23041 aatgagatgt ccgtacgccg ttgatctttg cattttttat gatagccaaa tctttttcca
    23101 tatcttctat agtgatggcg cgtccttttt tcggatgcca ctcatgtcga tttaccccgt
    23161 tgataatcat ccgttttcca ttgaagtaga tgattttatc tttgatttcg attttacgaa
    23221 aaccgaagga atagtcaata tactccgtta ctcttttttc ttcgtctatc gtctccaatt
    23281 caagcttata taaattgggc tgaaaatgcc cccacagtgc cacattttca aatccatcga
    23341 tgcgaatcgt catttgctct tttaacggat attgctgttg atagatttcc ttcccatcag
    23401 gatctagtat cgctacattc agctgactgt ttttggctac ttgctcaaaa gacaaatcta
    23461 cttcaagttt gccattttga catttgtcat ctaagcgagg tttgatccaa agatcttcta
    23521 aatggttttg tcctcgagca actaagaaga catctctgaa gatcccaaaa aatcggaaaa
    23581 agtcctgatc ttccaagaat gcagctgtac ttcttttgta aacctccaca gctagaatat
    23641 tttcttcttc caaataaggt gttaaatcaa attcagacgg agtgaacgaa tcttcggcat
    23701 acccgataaa atgaccattc aaccaaataa acattgcttg ttctacacca gcaaaatgaa
    23761 tcgagatttg ttttcccttt agaccttctt ctaatcgaaa agtcgtcaaa tacgaaccaa
    23821 ccggattgtc tttagcagct gaaaagatcc cttccgcctc tttctcaggg cttgtcgtga
    23881 aagggggacg gcgaaataca tgtccctccc acggatacat cgtattgatg taatggatct
    23941 tgtcataatt tgctaattca atatgactag gaacagtgat ttcatcaaaa gaacaggcat
    24001 caaaatcttt tttataaaat gtttttggtc gtaccgaagg atttggcgaa taacaaaaac
    24061 gccatgatcc atttaaagac tgtttaaggg aggatgtggt ttctacttct tcccttgttg
    24121 cataacaaac atgatcgcta tgtgcatttg attggttcac tctaaaaact tgtggatcat
    24181 ctaaccattt tatatttgct ttcatacttt tttccttctc tctcataact ttttgaaatg
    24241 aagcagttct gtttggaaat caccttgaaa atcggggaag taaagtccaa ctgtcatcaa
    24301 ttcggccccc gaaaatatcc gcttcgaggt ctgttcctga taaagtgcct catcatctaa
    24361 ccccatcaga tatacttcat ggaacgctgg ttgagctgaa gctagtatcc tccctaaaaa
    24421 cacaatagct tcatcttgtt taggagacac aaacatccat gcggcaatat tttcttcaaa
    24481 aggactcgcc aaacgataat aatggccata ctggatcacc ggacgaatcg tttgatagga
    24541 tataatttgg tcttttactt gcttttcttc ttcaggagac agctttgtca aatccagttc
    24601 ataaccaaaa acagcgctca tagctgtttc tcctcttgtt ttcaacgaag tagatctgtc
    24661 ggtctgatga tttggtacag ctgatacatg agctgtgatg gacgaaatcg gataagcaag
    24721 gcttgttcca tattggattt tcatacgctc cactgcatcc gtgttatcac ttgtccatga
    24781 ctgcggcata taataaataa acccggcatc aaaccgtcca cctccaccag aacagccttc
    24841 ccataagatt tccggataat cagttgtcaa atgttcaagt agttcataaa gcccgagcat
    24901 ataacgatgc gctacttctc cttgtctctg tgggtcaagg gtaatcgaat agacatctga
    24961 aagcgaacgg ttcatatccc atttgatgta gtcaagagga atcgtatcta aaatctttct
    25021 catttgtttc tcaatcgtct gccgaacatc taaccttgta aaatccagta catgttgtga
    25081 tctggaagca gaaggcattc tacctggctc ctgcataaga aaatcaggat gtgttcgata
    25141 cagttcagaa tcaaccgaga tcatttcagg ttcaaaccat aaaccaaatt tcaatccttt
    25201 gctatgagca taatctgcta tttcacgtaa tccatttgtc aacttgcctt catactcgaa
    25261 ccagtctcct aatgaagaat tgtccgcatc tctttttcca aaccagccat catctaatac
    25321 gaacatttcg atgcctagct cctttgcttc atcaatgatc gccttgattt tttcactatt
    25381 gaagtcaaag taagtggctt cccagttatt gaccagaatc ggccgttcag caaattgatg
    25441 acgacctctt gcgattcttt cacgtagcaa ctcatggtgg atctgactca ttttgttcaa
    25501 tccctgactt gagtaagaaa ggatcatttc aggtgtttga aaagactggc ctgcattcaa
    25561 ttcccacgaa aaacgatggc tgttgatccc accgacgact ctcaattgat tgatctgatc
    25621 tttttcaagt tcgaaagaat ggttgccgga ataaacaaat tgaagtccga tcgcttctcc
    25681 agtattttct gtcgtatgat gatgaactaa agcaataaag ttattcatat ggtgactgct
    25741 cgttcctcga cggctttcaa atacttttgt tccagataaa acagattgcc gtgaaatctg
    25801 tctttcattg acatgtgctc ctggcaaagc aatgacttca tcaaatcgcc tagtatgggc
    25861 aaagtctagc tggaaactag ccgctttttc tagtttgatt gtttctccgg tttcatttct
    25921 tatctttaca gatcgtgtga tcactgctct atcagtaaat atggtataga acaaatcgaa
    25981 ataaatcgct gtttctctat ccttaagtga aatcaccaac gtttgtgcct ctgacttgtc
    26041 catcacataa gtttgaggca agccttcgat ctctggcttc ccatctgtaa tataatggct
    26101 gtcatatctc agatccagta agtctgatcc attcttgcgc tgaatgatcg atgcgggcgt
    26161 tcgaaagtcc atgctgccat gagaagagta ttcttgcggt agtgtatctt tcgacagaga
    26221 ccggtcagga tttaagggaa catttcctga aaatccacga tctcttcggg gataattttt
    26281 attgtctttg tactgcttga ctggtttgcc aaaataaata tgcgccaata cattgctttc
    26341 ttctatcttt atcagataag aaagaaatgt atttcgtaaa tggaagactc ggctcttttc
    26401 atcaaataag atgaaatttt tatctgtttt tctatgcgta atatccatca tttcactccc
    26461 aacaaaacgg aaaccgttta ctttttttta taattcctgt atactgttta ctaaacaatt
    26521 ttgtcaataa aaatttttta tttttttgag gagtgtgtaa aaatggttgg aattagagat
    26581 gtcgcacgaa aggcaaatgt ctctcctgct acagtttccc gtgtgttgaa taacgatcag
    26641 acgatccatg tggctcagac tactcggcaa cggatagtag aggcagctgc tgcgttggat
    26701 tatgacatca gtactcgtaa atatatcaaa aagagaatgc cttcgattgg tgtgatctct
    26761 acgatcggca aagcgagcga agaacaggat atctattaca aacatttgcg agtagggatg
    26821 gaagaagaag ctcgacgtct gcatttaggg atgaatcgaa tctataatct atctgataat
    26881 cctaaacaat gggaagatct agatcagtta ggtgcggtga tcgttgttgg gactgttacg
    26941 aaagaatcga ttaccgcctt gttaaaacaa aacatgcaaa tagtcgtcgt ggataaccct
    27001 gacatccagc aagaggtcga tatggtgtat gcagatcttg aacggatgac taaaaccgta
    27061 ctggaattgt tcttagaaaa tggtcataaa agaatagcct atatcggggg atataaagtc
    27121 gatatggatg aattcggtgg gaagcagatt tctataaatg aaaagaggat gcgtgcctat
    27181 cagcatttta tgatcgatca tcacttagaa aaatatatgt cttatcatct aggtggctgg
    27241 acagaaaaac aaggtgaggt acttgcgact aatctattag aaaatgaatc ccctcttcct
    27301 actgccattc ttgtcgggag cgatccaatg gcaattggtg tctatcgggc attgaaacaa
    27361 aaaaatatcc aaattggaga agagattgtt gtagcaagct ttgataatat cgagacaatc
    27421 gcaaacctct cccctggctt gaccagtgtt caaatcaatg cgaaatcaat tgggcaagca
    27481 gctgttcgaa tggcattgga gcgaattgac ggtatacggg acgaaccaat cattttgact
    27541 tatcctgcac gattgattgt tagggaaagt tttatcccaa gaacaaaaag gtattgaaag
    27601 agaggttgtg aaaagccgct tcgtttcttc atactcaaaa cgaaacggct ttttcacaac
    27661 ctcttactta tacttattca caaaatccaa tgtctgctgg taacaatttt gcgattcttt
    27721 tagcgtgtaa tcaaattggt attcatgggt aatctctttt tttgtatctg tataaaacaa
    27781 ggattgcacg ggtaccttta attctgtcaa ccgattttct aatgcttgac cttgctcctg
    27841 gaatgagtat gcatttccgt cagttatata cgttggcgga aattccttag aaacatggtc
    27901 agccacacta gcttctttca attgctcact cgttttccaa tcttttgttc caatcaacga
    27961 ccacgcaacg gttttgacga aaaacttcat aaatcgatca tccgaatgct gctgtgctgt
    28021 tagcttcaaa tcaacaggtc cgcaataaga aatggttcct ttcagactat cttttggaag
    28081 tagctgtttg attttcattt gtttagcata cgcatcattc gtttgtatag ctgcatattg
    28141 caatgcgatt tgtgaacctg cactatctcc accaaaaaat aattgttcca tgtctagcaa
    28201 tttgtcgtct ttttcttcag ctaataaatc tttgatcagt tcattcactt gaagaacttg
    28261 attagggtat tctgaatcag gcgctaattc ataattcatc gcaaccactg cgatgttggc
    28321 gtctgaaact aattttgtcg caaattcttt gacacccgat ttatctccgc cgacgaatcc
    28381 tccaccatgt acccaaaata gaacaggaac aggccccttg ctgttttttg gatagtagat
    28441 gtcataagta ttctctttat acttggactc atacgtcaaa tctgtttttt tcgccacgtt
    28501 tttttctgct tgatcgaacg tttttttatc agtgatttga acagtatttg aaaacatatg
    28561 gttgatcaag aatgctccag gtctggggga aagctggaag gcaagaaaga ttgcaaacgc
    28621 gatcccaaga atgattccga agaaccatcc gataaattta agaattttct ttttcataag
    28681 gctccctcag tagttagtat taagcattct gattatacca tagtgttatt tcctatcaag
    28741 ctctcctaga tgatttaatg attttcccca aacttcttct gcaatgcttt tgacaaaaga
    28801 aattttttcc attgttcttc aaacaaaaca ttttcttctt ctgttgaagc aaatccacat
    28861 tgaggactta agcacaaacg atccaaaggc agatatcgtg tcgcttcgta gatccggtca
    28921 atcacttgct ggtgctcttc catttcgcca tttttcgtta cagtcaaaat ctaagacaac
    28981 taatttatct tcagagactt tctctaaaag aacaaaactc ccagcacgtt cactatcgta
    29041 ttctaaataa taggcacgga cattttcttg agtaaaataa tgggtctgct acgtcgtcat
    29101 cccccctgat gccgccaaaa tcgaataata atttccgcgt caaacatgta tatttagtgt
    29161 tgaatcattt gagaatatga aaataaaaat ctttttgatc aatcagttaa tcaaattgaa
    29221 ataacgaaaa atctgagaca gaatgacgtt cattctgacc cagatttgat tttgcacaac
    29281 tcaacagtcc ttattggcaa atcgccaaaa taatgtagac taactaaaaa gactaagaaa
    29341 caataacaaa agttaacact tcttagtctt tctttagcac gtctatttat taaagatttt
    29401 agggcctttt ctcatgtttt tacgatcatt cttaggtgtt tccatttgtt tggatcggcc
    29461 tccaccttgt ttctttttga tcaattcttt cattttttct ttttggtcca tttttattct
    29521 cctttaacga atgaagcgac taattcgata tgatgggttt gcgggaaaag atctacaggc
    29581 tgaactttct tcacggcata acctgcttct gcaaataact tagcatctct tgcaaaagtt
    29641 gccggattac atgagatata gacgattcgt tctggtgaca tttcgattgc tgattcaata
    29701 aaatgatgat ctaatccttt tcgtggaggg tcaactacga tcacagatgg tttgatccct
    29761 ttttcttgcc attttttcat aactttttct gcttttccga cttcataatg aacattttcg
    29821 attttattat ttaaagcatt gaactgtgca tcttcaattg cttctggaat aacttccatc
    29881 ccatacacct tatccactct gtctgctaaa gacaagccaa tcgtacctat tcctgaataa
    29941 gcgtcgatca caatgtcttt tttatctaac tcggcaaact cgatggctgt ttggtacaga
    30001 tactctgctt gttgagtatt tacttgataa aatgactttg ctgagattcg ataggttttt
    30061 cctaatagct gatcttcgat atagtctctg ccatataaaa ctttttcttt gtctcctaaa
    30121 atcacatttg ttttttcttc attgatattt tggatgaccg aaaccacttc tggcaattct
    30181 tcatgaatag cttcagcaat ttccttggat ttaaagaatt tttctttacg agtaactaaa
    30241 acgaccatca tttcatggga ataatgacca cgacggataa tgatgtgacg aagctgtcct
    30301 tcattttttt cttcattata tcctcgtact tggaaccgtt gcaagatatc tctgactact
    30361 ttgattgctt gatcgatgac aggatcttgg atataaaaat cttcgatagg gactagatca
    30421 tggctattct ttttgtaaaa accggtggct aactgtccat cgatacggcg aacagggatc
    30481 tgtgctttat tacggtagcc gaatggttct gccattccga ccgttggaag tacaggaact
    30541 tctggcatct tagcaatctt cgccattaca tgctctactt gtttttgttt gaagacaagc
    30601 tgttgttcat acttcaaatg ggctaaaggt gcgataccta tgcgaagtaa tgcattgttt
    30661 tctacaggct gacgatcagg tgattgtgta ataaatgttt cgacttttcc atatccaaac
    30721 ttattgcctg cttttactac tagtatttct acttcttctc cagggagtgc attttcgata
    30781 aataatggat aaccatcgat ttttgctacc cccattcctt cataactcaa gtcgacgatt
    30841 tcgactgtat atcgttcgtt tttctttact atttgctgtt ctgtcatatt ttgctccttt
    30901 aagcctattt ttcaatccta ctgaactcta ctataaatca gctaattaca caactaaaac
    30961 cggcactgac cttactacgt tcagcaccgg cttttgatta attcttttga ttaggtgttt
    31021 cgtctgctat ttttccgtca ccgtcgatat cttcatccgt taaacgttcg acttctttta
    31081 cgaattcttt gctgacaacc tctaattctt cttcatctgt acctgtgatt gcttcatcag
    31141 gaatctgatc ggtattggca aagaactcaa tgtgttgatg aagattttgg aaatgcatcg
    31201 gtgcttcccc gccgtattca ccatctaagt tgatcattac tggtcgattg atatcatctt
    31261 ctgtttcaac gaacaaactg cttgttttgg tatagattat tcttggatca tcgacatgtc
    31321 gaccgccatt cagcatgagt gctgcaatat gcaagatctc aaagatattt gctgtcttca
    31381 caatgattaa agaaaacttc ccatcatcca atttggcatc tggcgcgatc tgttcgaatc
    31441 ctccaacaga gttcgtcagc cctaggaaaa acatcgaagc atttccgata tattcccctt
    31501 catcatactt caagcgcatt ttgattggct ttactcgagg gagcatctct gctcctttcg
    31561 caagataagc aagataacca aaaatactct ttagttctga tggaacttca taagtcaatt
    31621 ctgtcaaata gccacctgca gcgatattga tgaagtaact gttatcagaa aggccgatgt
    31681 ccatttttac ggtttggtgt ttttttatca cttctgctgc tgctttaata ttatctctag
    31741 gaatttttaa tgctcgagca tagtcgttcg ttgtaccagc tgggatgatg gccattttag
    31801 gacggtgttc aagcggagcg attccattaa ccacttcgtt gattgtgcca tcgccaccag
    31861 ctgccactac tagatcaaaa cctagttctg cgactctaca tgcttcattc ttggctgaat
    31921 ctggttctgg tgtagttgca taggcgctgg cttcaaatcc tgcttcttct aatgcctgta
    31981 ggatatccgc caaatttttt ttcaataatt cttttcccga ggtggggtta taaattacgc
    32041 gtgctttttt cattttcagc ctctatcgtt tcgctaattc atctttaagt agcttattca
    32101 cgacaccagg atttgcttgt cctttagttg ctttcatgat ttgaccgact aggaagccaa
    32161 ccgcacgatc ttttccattt ttgaaatcat cgacagattg ttggttgtta tccaaaattt
    32221 cattaatgat tggcagtagt ttgttcggat cagataattg cacccaacca tttttttcaa
    32281 ctacttcttg ggcttctccg ccatttgtga tcaattcacg gaagactttt ttggcgattt
    32341 ttgagctgat cgttccatcg gcgatcaaac gaatcattcc tgctaagttc tcaggtgtca
    32401 attttgtctc agctaattcc agtttctcac tgttcaagta agcagaaact tcacccatca
    32461 accagttaga tgcttgcttg gcatctgcac cattttccaa aacagcttca aaaaaatcag
    32521 acatttcttt tgtaagtgtc aataccattg cgtcatactc tggaagtcca aactcacgga
    32581 tatagcgttc gcgacgagaa gctggcatct caggcaaact agcttttact ttttgaatcc
    32641 attcgtcatc gatcacgaat ttcgggatat caggctctgg gaagtaacga taatcactag
    32701 acccttcttt gacacgcatc aaaactgttt tattcgttgt ttcgtcaaaa cggcgtgttt
    32761 cttgttggat ttctcctcct gaaagaagaa cttttgcttg acgtttttct tcaaaagcta
    32821 aacctttttt cacgaaagat aaagagttca agtttttcaa ttctgctttc gtaccgaact
    32881 cttcttgtcc ataagggcga agagaaatat tggcatcaca gcgcatagag ccttcttcca
    32941 ttttcacatc gctgacttct gtaaattgga taattgatcg caatgcttct agataagcgt
    33001 atgcttcttc tggtgaacgc atatcagctt cagaaacgat ttcgatcaaa ggcgtgcctt
    33061 gtcggttcaa atcaacgtaa gaataaccgc caataccatg catgtttttt ccagcatctt
    33121 cttctaaatg gacacgttcg atccggattt ttttcttttt accttctact tcgatttcga
    33181 tccatccgtc atgaccgatt ggttgatcaa attgggaaat ttgatatgct tttggattat
    33241 ctgggtaaaa gtaatttttc cggtcaaaat gagtatcttt agagatttca cagtttaatg
    33301 ccaaagctgc tttcatcccg aattccagcg cagctttatt cattactggc agcacgcctg
    33361 gataacccca gtcgatcacg tttgtattgc tgtttggttc tgcaccaaaa tgagctggcg
    33421 ctggagagaa aatttttgag ttggttttta actcgacgtg gacttctagt ccaatgactg
    33481 tttcaaaatt cattcgtctt tccctcctaa aatagctggt tgttgtttat ggaattctgt
    33541 tgcttgttca aacgcatatg ccgcttgata catcgttttt tcatcaaaat aattaccgat
    33601 gatctgcaat ccaactggta atccttctga taaacctgcc ggtacagaca tacctggaag
    33661 tccagccaag ttgacaggga tcgttaagat gtcactcatg tacattgtga ttggatcgtt
    33721 aatgttctca ccgattccga atgcaactgt tggtgaagat ggcccgatga tcaagtcata
    33781 atctgcaaag actttttcaa aatctcgttt gatcaatgta cggacttgtc ctgctttttt
    33841 gaagtaagcg tcataataac ccgcgcttaa ggagaatgta cctaacatga tacggcgttt
    33901 tacttcttca ccgaatcctt ctgaacgtga gttgacatat acatcatcta atgatttcac
    33961 attttcagag cgataaccgt agcggatacc atcgaatcgt tgcaaattgg aacttgcttc
    34021 tgatgaggca atgatataat aaacagccac accgtatttt gaatgaggca agctgacttc
    34081 ttccacaaca gcacctaatg aacggaatgt ttctgctgct ttcaatacag cttcttttac
    34141 gccaggggcc acaccttcgc ctaagtattc tttaggtaaa gcaatcttca ttcctttgat
    34201 gtcgcctgtc agtccctctg caaaatcagg aactgaaacg cctgaagaag taccgtcttt
    34261 ttcgtcataa ccgctgatag ctgtcaatgc taatgcatta tctttcaccg tacgggtcat
    34321 tggtccgatt tggtcaagag atgaagcaaa ggcaatcaaa ccaaaacgtg agacacggcc
    34381 ataagtaggt ttcatcccta cgatgccgtt aaaagctgct ggttggcgga tacttccccc
    34441 agtatcacta cctaaagaaa ctggaatttg tccagctgca actgctgctg cagaaccacc
    34501 agaagagcca ccaggtactt ttgttgggtc ccaagcattt tttgttttct tgaaataaga
    34561 agtttctgta cttccaccca tagcaaattc atccatgttt agcttaccaa cagggatcat
    34621 atctgcttga tagattttat ccatgaccgt tgcatcataa attggttcaa aattgtaaag
    34681 gatcttagat gcagcggttg tcaagatatc ttttgttacg atattatcct tgataccaat
    34741 tggaatacca gcaaggacgt tgctttcatc aatccctttt tcatctaaag cttttgcctg
    34801 ttccaatgct ttttcttcgt tcaatgtaat gaatgcctcg acctcaggct ctgtttcttt
    34861 gattcgttca aatgttgttt ttgtcaaatc ttgtacagtg atttctttcg atacaagcat
    34921 atgatgcagt tcttctagag aacgattgtg taattctgtc attatgcgcc agcctcccca
    34981 ttatcgatga ttgcgggtac tttgataaag ccgtcttctg tttccggcac atttttcaat
    35041 aattcatcgc ggttccatcc agcttgcgcc cgatcttgtc gcatcacgtt tactgtttca
    35101 accacattcg acgtaaatgg aacaccttcc gtatctactt cttccagcat ttcaaccata
    35161 tcgatgattt tacctaattg gtcggtaaaa cttgcaagtt cttcatctgc gaacgcaagt
    35221 tttgctaatt tggcaacgtg tttcacttgt tcttcactaa tcgccatact accccttctt
    35281 tctaaacgag ttaaaaactt tgctctctat tttaacacaa gagtgccgtg tctgaaattg
    35341 tttattgaaa tttgtctgaa aaaactcatt tttcattctt ctactgagcg acttttagcc
    35401 aatgcatctg ccatttgatc ttctgtccaa acttcgattc ctaattcttg tgctttggtc
    35461 agcttgctgc cagcatcttc tcctgctacc acgatatccg tttttttcga tacgctacca
    35521 gtcactttcc cgccaagatt ttcgatcgtc tcttttgctt cttcacgagt aaatcgtgtt
    35581 agtttaccag taaggactac tgttttttct ttaaaaggag actcgacttc ttgcaattgg
    35641 gcatttcgaa gccctttgta ttcgaaattg acgcctgctt gttttaattc attcatcaat
    35701 tcatggactt cttcgttact gaaataagtg actacactgt cggcaatgat atcaccgatc
    35761 gtatcaagcg caataatcga ctcatagtca cttttactca atgtttctag atcaccgaaa
    35821 tgttctgcta aaatttttgc tgctttcgct ccgacatgtc ggattcctaa cccgaaaatc
    35881 aaccgctcga ccgaattatc tttgctgttg tcgattgctg tcaaaatatt atttgctgat
    35941 ttttctttta ttttgtccaa cgtcaataac tcttcttctg tcaatttgta aagatcagca
    36001 acatctgcta ctagtttttt atcatacatc tgttctagta cgcgcggccc caacccatcg
    36061 atattcatcg catttcttga aacaaagtgg ttcaaacctt ctttcatttg agcagggcat
    36121 ttaggattga tgcaccgtaa ggctacttct tcatccaaat ggacaagttc acttccacaa
    36181 actggacaat gtgtaggcaa ttggtattct tcgctgtctt taggtcgttt atctaacacg
    36241 acttgcgaaa cttctgggat gatgtctccc gctttataaa tcagaactgt atctttcaag
    36301 cggatatcct tcaacttaat ataatcaccg ttatgcaggc tggcacggct tactgtcgtt
    36361 cctgctactc ggactggtgt catgatagct gttggcgtta ctacccctgt tcgaccgact
    36421 gtccattcga tgttttcgat tagcgtttca acttcttctg gcggaaactt gtaagctgct
    36481 gcccatctcg gtgctttgat agtaaaacca agttgatctt gtagcgagaa ttcattcact
    36541 ttgatgacga ttccatcgat ttcatagggc aaatccacac gtttgtcatg atattcttca
    36601 atgtatgacc acacttcatc aatcgaatga catagacgtt tttcccgatt cgtgtggaat
    36661 ccaatttttt ctagctcttc tagcgcatca tactgggttt ttgcttgcat aggaccgaaa
    36721 tcagctactg tgtataaaaa agtgtccaga tttcttttcg ctgtgatttt agaatctaac
    36781 tgacgcaaag aaccagctgc ggcgttccga ggattcgcaa agatatcttt tccctctgct
    36841 tctctgtctt ggttcaattg gacaaatgac cgttttggca tgaaacattc gcctcttact
    36901 tcgatgttca ttggttcttt taatttgatt gggaccgacc ggacagtttt taggttttca
    36961 gtgatgtttt ctcctacagt tccatccccg cgagtcgcac ctctcacgaa atttccatct
    37021 tcataacgca aagaaacgga aagtccatct atttttagtt cacagcaata gtcaactaca
    37081 cgtcccactg ctttttgtac acgttgatca aaagctatca gctcttcttt gctgaaaacg
    37141 tcgttcaaac tgtaaagcgg aatatcatga gtgacttttt caaaaccttc taatactttt
    37201 ccgcctactc gttgagtggg agaatcagac gtgatcaaat caggatattg ttcctcgatt
    37261 gctactaatt ctgcgtattc tttatcataa acatagtctt ccactgttgg tttatcttct
    37321 acgtagtatt cgcgagacca ttggttcagt cttgtacgaa gttcttctgc tcgtttagca
    37381 gcttcgtcaa tcgtcatttc atgttccatc atttaccctc cactactaag ctttttcaat
    37441 tggcgcaaag gcagccaaaa gacgttttac tccttgactt gggaaagcca catctagttc
    37501 tagatccttc gacgtcccgc ttactcggac gactgttcct agaccccatt ttttgtgttt
    37561 gactttatct ccaggtttcc agctgttatt ttcccctcca gtagcacttt tagagggaac
    37621 aaccgctgtc tctggctgtt tatagctata tgctgttttt ttagcgaata caggcgtttt
    37681 tttaggcgta ggacgcatgc cttcgatttc cagtaactcc tgatcgattt cttctacaaa
    37741 acgagaaggt cggttatatt gcgtccgtcc atataatgtt ctggagaaag catttgttaa
    37801 atacaacgct tcttctgctc gcgtgatccc cacatatgcg agacggcgtt cttcctccag
    37861 ttcgctttct tccatcaatg ctcgagataa aggaaaaacc ccctcttcta gaccaatcag
    37921 gaatacaact ggaaattcta atcctttggc tgcatgcaaa gtcatcaatg tcacttggga
    37981 agcatcttct tctaaattgt caatgtcgga aacaagtgcc agatcattta agaagaccgt
    38041 tagtttttct tctggtgcat cagcgtcttc ttcattttgc tgttcgaatt gtttgtcgaa
    38101 ttcttgtgtg accgttaaaa attcctccaa gttttcgatg cgtgcttgtg cttctaatgt
    38161 attttggatt tttaagtctt ctagatatcc gctacgatcc aaaacctctt tcgtcaattc
    38221 tgtcactgtc aagtatggga tcatctgagt gacttcttgg atcatctctc caaatgcacc
    38281 taattgctgt cctgcttttc caccgatatt tgccagatcc acattttgcg ctgcttcaag
    38341 cagtggccat tcatgaagag aagcaaaact gcgtagtttt tcgatagatc ctggtccgat
    38401 gcctcgtttg ggcgaattaa cgatacgctc aaaactgatt gaatcttgag gatttaccag
    38461 cacattcaaa tacgctaaaa tatctttgat ttctttacga tcgtagaact tgtgccctcc
    38521 aaccatttta taaggaatat ttgctttcaa cagagtttct tccattacac gagattgtgc
    38581 gttcgtccgg tacaagatgg caaagtcgcc gtaattccga tgattgcttc gaatctcttc
    38641 ttgcatccgg tctacgataa aacgggtttc gtctcgttca ttatctgcac ggtagtaagt
    38701 gatcttgttt ccttctttgt tttctgtcca caagtttttt ggttttcgat tgctattgtt
    38761 ttcaatgact tggtttgctg cgctaaggat gtttttcgtg gagcgatagt tttgttctag
    38821 caaaattact gcagcatctg gataatcttt ttcaaaatcc aagatgttct gcatatctgc
    38881 accgcgccag ccgtaaatac tttgatctgc atctcccacg acgcacaggt tcctaaatct
    38941 acctgccaat aaattgacta gcgtatactg tgcgtgattt gtatcttggt actcatctac
    39001 atgaatataa tggaatttgt tttgataata cgtgagtgaa tcaggatgtt cttcaaataa
    39061 tcgaatcgta ttcatgatca ggtcgtcaaa gtccatacat tgattatatt gaagctcttt
    39121 ttggtaagca gcataacatt ttgccgcaat ctcttcgtaa taactgcctt gcatttttgc
    39181 aaaatcctga ggggtctgca ggctgttttt tgcttgagag attgttccta atatacttcg
    39241 aggatcatat tttttcggat caatgttcag ttcttttagt atccgtttca tcaaggtcag
    39301 ctgttcagaa gaatcgatga ttgtaaagtt ccggttatac ccgataaaat caacatctct
    39361 tcttaaaata cgcacacaca tcgagtgaaa agtcgatacc cagacatctt cgcctccaga
    39421 tgcgagaatc gcattgacac gttctttcat ttcttttgca gccttattgg taaaagtaat
    39481 cgctaggatg ttccaaggat tgacttcttt ttcctcaatc aaataggcaa tgcggtgtgt
    39541 cagcactctc gtctttccac ttcctgcacc tgccattagc agtaatggtc cgtcagtgtg
    39601 aagaacagct tctttctgac gggggttcat cccgtttaat aattcagctt tggatgacat
    39661 taacaacatc ctttctttta tcaatcttgt taattatagc attttcttgg ctttcattaa
    39721 actagctagt tcaaaatcat ttgtttcttt ttatggattt ttccttgtca attcgttgat
    39781 ttcgcgctaa attaaaagaa agcgaggcgt ttgaatggaa aaaaaaggaa aaatcaagaa
    39841 attagataag actcagatat attttattct aaaaggaata tttgttggac tgctgacagg
    39901 aatcgtcgtt agcctgtttc gattatctat cgaaaagcta agcgatttag ttcgaagtat
    39961 ctatgaaatg agtcgggaac agccgattta cttaattggc ctagctattt gctgcatcct
    40021 tgcagcattt ttcgtgggtt atctggtcaa aaatgaaccg gatatcaagg gaagcgggat
    40081 tccacaagta gtgggacagc ttcgcgggga attatcaatg aactggttct ctgttttatg
    40141 gaaaaagttc attggcggtg tactttcagt tggtgcgggc ctttttctag gtagagaagg
    40201 accttctata cagcttggcg catccattgg acaaggagct ggacaattgt ttcggagtcc
    40261 atcttcagaa gaaaagattt taatttctag cggtgcaagt agcggacttg cagcagcatt
    40321 caatgctcct atcgcaggat tactctttgt tttagaagaa atccatcact ccttttctcc
    40381 acttgtttgg ctgacatcat ttgcttcagc aatcactgct aacttcgtct ctctttactt
    40441 tttcggcttg agacctgttt tatatctagg tgaccttcct tctttgccgt tgaaatatta
    40501 tggttcctta gtgatcttag gagtacttct aggcatctta ggttttattt atcagaatgt
    40561 cttgctggct ttgccaaaat tttacaaccg attgccttta ccagcttatt ttcacgggtt
    40621 cgttcctttc ttgctgattt tgccaattgg ttatttatgg cctagtactc taggcggagg
    40681 caatcaaatc gttttagctt ttggacatgc ttccttgcca atcattatga ttatcggttt
    40741 gtttgttctt cgattcgttt tttccatgat ttcttacggt gggggtcttc caggggggat
    40801 tttccttccg atattgacat tgggggcttt aatcggtagc ttttatggaa acattatggt
    40861 tgatttcggg atggatccaa tctacatcaa ggattttgtc gttattgcca tggctggtta
    40921 tttcacagcg attggaaaag caccgctgac agcaattatt ttagtgacag aaatggttgg
    40981 ctctcttaat catctgatgc ctttaggatt ggctgctctt gttgcttata tcgtcaatga
    41041 ccttctcggc ggtaatccaa tttacgaatc attattggaa cgtctacttg gaaatactca
    41101 gaccgcctca ctaaaaggtg caaggacaac ttttgatttt ccagtaacgg cagagagttc
    41161 tttagacgga ttaatggtac gagacttcaa ttggcctaaa aatatgctgc taatctctat
    41221 acgcagagga aatcaggaaa tcttgactca cggtgatacg atcatgaatg taggagatat
    41281 tttggttatc ttgactgatg aagcaaactt gccaagaatc aagcaggaaa tcgaggctaa
    41341 atcttctatc acaaatttga agcaatcatt ttaacgaaca tggaaaaact attttacaac
    41401 aactattcat ctacactact tgattctata tgattctata aaaaaagaag aggctgggaa
    41461 aacccagcct cttctttttt atttctatgc ttctgctact gcaggagctt ttctcaataa
    41521 accataaatc acaccagata cgactgctcc gatagcaata aacaccaaat acatgaccgg
    41581 gtgactcagt aagaatacaa cgaaaatccc gccgtgagga gccagtaatt tgattccgaa
    41641 agctccgact aatccgccag ttaaagcaga accaataata aaacttggaa tggcacgaat
    41701 cgggtcagct gctgcaaatg gaatcgatcc ttccgtgaca aacgatagtc ccatcacgat
    41761 gttcgtcaaa ccagcatctt gatctgtttt gctgaattta ttcttaaaca aacgtgtagc
    41821 aacgaaaatt gccaatggag gtaccattcc tccagccata acagaagcca tcacgacact
    41881 tccgccagtt gcaacagtcg tagctaaagt tgctgtacca aacacataag cggctttgtt
    41941 gattggaccg cctaaatctg ccgccatcat tccagctaat aatgcaccaa gtaaagcagc
    42001 attcgaacca ctcatacttg tcaagaaacc gttcaatgca tcattgattg ctttcatagg
    42061 gacattgacc agcaacatca agaaaccagt aatcagtaag ccaaacactg gataaaagag
    42121 gatcgtttta attccatcca atgattttgg taagttcttg aataatttac gtaaaaatac
    42181 gatgacataa ccagctaaga atccaccaat cagtgcacct aagaatcctg caccgcctgt
    42241 attagctaat gcaccagcag caaatccaac gatcaaacca gggcggtcac cgatacttga
    42301 agcaataaat cctgctaata ccggcagcat gaaaccaaat gcagcttgtc cgatttggtt
    42361 gaaccagcta gcagcttgat tgtaattccc tagatttgat aattgatcat gtggcacgcc
    42421 aataaattgg tcgatcatga aagaaagcgc aatagcgatt cctccaccaa taacgaaagg
    42481 taacatgtgt gatacgccgt tcatcaaatc tttgtaaatt ctttgtccga tagtaccatc
    42541 cgcatttcct tcttctgaac tgctttcttt tccatcacca tggaatactg gtgcagtacc
    42601 actaagggct agattgatca actcttctgg tttacggata ccatcactga caggacgatt
    42661 gatcagttct tttccgtcaa agcgattcat ctctactttt ttgtctgctg caacgattac
    42721 accttctgca cgggcgatgt cttcagcagt caatcggttt ttgattcctt ctgaaccgtt
    42781 cgtttcgact ttgatctcca cacccatttc tttcgctttt ttcttcaaag catcttctgc
    42841 catgtaagtg tgagcgatcc ctgttggaca agctgtaacg gcaacaacga attttctttc
    42901 tggatgagca gaagcttctg cagcttgtgt ttcttgttct gctttttctt ctgcttcttt
    42961 ttgctcttca gctgtttgga acaagtcttg gacttcttct ggtgtatttg cttcttttaa
    43021 ttttcctacg aaatctgcat caatcagcaa tcgagatagt gctgctaatg cttgcaaatg
    43081 tgtgtcgttt gcgccttctg gagctgcgat catgaaaaac agataagttg gctgtccgtc
    43141 taaggcttca taatctactc ctttttggct ttttgcgaat aaaactgttg cttctttaac
    43201 tgcactgttt ttcgcgtgag gcatggcgat tccatcacca agacctgtag aagtttgtgc
    43261 ttcgcgggct agaattcctt ctttatatgt ttcgatatcg gaaatccggc cggcatcaaa
    43321 cattttttgg accatttcat cgattgcgcc ttttttatca gtcgcttgca aatccatgat
    43381 catcgcttct ttgaccatta aatccttgat attcatgatt atcctccttg aaactaatca
    43441 atttttgtga tttcaacttc tggcagcaac tcatcaatca attcgcgagc tgccaagtca
    43501 tcagaaaaag ctgttgcgct tccgcaagca actccccatt taaaggcttc gattggatct
    43561 ttcgtacggc tgtaagcccc aataaagcca gcaatcattg aatcccctgc acctactgaa
    43621 tttttcaatg ggcgaactaa tacattcgtt ttataagttc cttgttccgt gaagagcaaa
    43681 gcaccgtctc cagccattga gatcagcaca ttctgtgccc cttcagctag taattttttc
    43741 ccatacggaa tgatttcttc tacagattgg aaggttgtgt tgtaaagctc agctaattcg
    43801 tgattgtttg gtttgatcaa caacggtttc ttaacaagtg cattttttag gtcatcacct
    43861 gttgtatcaa tcacgaaatc tgcacctttt tctcgaatga tatcaatcag ttcttgataa
    43921 aatccttttc gcaggctagc tggcgcgctt cctgacaata cgacgatatc tccttgtcct
    43981 actgttccta atgcttgctt caactcatcg atttcttcat ctgaaataga tgggcctaag
    44041 ccattgatct cagtttcagt gtctgatttt agtttgatat tgattcttgt atcttctttt
    44101 acggttgtga acaaagactg gatttgttct ttttcaagcc acttcgaaat aaattcgcca
    44161 gtgaaccctc ctaaaaaccc tagtgctgta gattgagaat cgatccgttt caaaatacgg
    44221 gaaacattga tcccttttcc tccaggtaac ttgaagtctt ccttcattcg gttcaaatcg
    44281 cctagtttta acccatctac gcgtacgata taatcaattg atggattaag tgttactgtg
    44341 taaatcattg tattgcctcc ttaattgttg tcttttcacg gaattcatta acgaattcta
    44401 atggacaacg ctcggttatg atcgtggctg cttccaaagg tgcaacagag gtgaaggaca
    44461 actggttgaa tttggtatga tcaatcaata caaatgtctc ttcggaattt ttcatggcta
    44521 aacgtttaac agctgcctct tctggatccg gagtcgtgaa tccttgttca agatgggcac
    44581 cgttcattcc cataaatgct ttattaaaat gaaaattttt tagttgttct atcgcattag
    44641 atcctaaaac agcattagtt gccagcttta tcgttccacc taaaataatg gtagcaagct
    44701 ccaaatcaac taaatttgcc gcatgttgaa cggaatttgt cactactgtg atctgttttc
    44761 cttgcagaaa aggaatcatt tctaacgtag tagagccagc gtctaaataa atgacatctt
    44821 cgtcattaat ggtttcagct gccgatttgg cgatttgctg tttttcgtga acgtttttga
    44881 gtgatttttc actcatattc tgctcaaaat tcaataattg ctctttctta gctccgccat
    44941 gaactctttc tagaaaccca ctattctcca gctcttgtaa atcacgtctt atcgttgatt
    45001 cagaagcatt caaaaggttt acaagatctt gtgatttcac gattttctgc tgttcaagca
    45061 agtctaggat tttttgtctg cgttcctctg taagcatttt cacgatcacc tcaatacaaa
    45121 taatagcata ttttttttct actagcaata taaatcagtc aaaaacgttc ataaaaatta
    45181 taaatcagtc aaaaaaaaat aaaaaaccag tcagaaagac tggtttttta tttttttgtc
    45241 tatcatcaag ccgttttatc gttttttatt ttcgcttctt gtttttttca tataaacaga
    45301 agcaggtttt ctttccttat gaagaagata ataataaagt cccatcccgc caaacatgac
    45361 caaaaaaccg atgactgatc gaataggaaa attaggaaca aaaaagagac agacggataa
    45421 taatgatgcc ggaaccatta ttcgataata gagttgttta tccattttct tcacctcata
    45481 ttttttgtaa agtaatcaat tgttctaatt ctcggtaatc taaaaattgc acacgcagat
    45541 gctgctcacc tttcttgaca tcgaattgga tacttcgccg ctttttcttt attcgtttga
    45601 aggtaacatc agagagtcta tgtttgattt cctttaacgg taattctgac agggattctg
    45661 aaggaacata gattcttaag gataaaagat actcttgata tttttcatct gttacctcta
    45721 cttctagata ttccctaata taataacaat aagcttcgat tccattatcc aaagcaacag
    45781 tcggatcgat aactttatag cacatcatgc gtgatcgatt ctcgtcaacc caaggaaaat
    45841 acgcctgagc atttagtgtt ccgcgaacac gcctttcttt ataatctcct tgttggatat
    45901 cataactttt tgttacaaaa aatgaagcat tctttttagg aacaggttgc atttgtgcat
    45961 tctgatactg gacagattgg atcgtatggt cagtgtaaat gtattcaacg aatggcggtt
    46021 tcaataaacc gacaccattg tcccaagcaa gatgatcaaa cccaagctgg tccctaaaat
    46081 aggattgaaa catttgattg tttggctttt gccagttaaa ggggtctcga taaagctgga
    46141 tacatatgac ttcgttttct aattccttca ctttttctac ttgttggatg tcgagaaaaa
    46201 tcggtttagg gatgatttga tgataatagc gactataata catttcccaa cacgaagtga
    46261 aacataaacc ttggtaaaaa agatcgtatc ctggaaattg gttgcaatcg actactactt
    46321 ccccgttttg ttgtttcatt ttaggtaaat gttcgatttc atcctttttc tcaaacaata
    46381 gccgttgaga gatctgtttt gtattgtgat agataaactc gctataagct cgcatatagg
    46441 cgaacacccc atgagcaatc atttttttct gaatgaagtc tcgaatcaaa aattgctttt
    46501 ctttgaaaac agagatagac aatacatttt ttatcaataa ttgattatct tccaagcgaa
    46561 aactacattc gttctcgtca tttcctacaa ctaaccaact ttcgtctctt accgatgcta
    46621 gcattatttt ttcttgaagc tttcgaagat catacggatc cgcttgttgt gtgaaagagc
    46681 ggataaatgt tggctggatc aacagacgtg tcaaaatagc ccaatcgctt atttcctgtt
    46741 ttttttcttc ttcatttaag tctgtgagct ggatagtcat caataattta tcttcgccaa
    46801 tcattttctc acctctttca aatcttttta taatttaatt ataaccaatg ataaagaaaa
    46861 actaaaactt tatgtcttag aaaccaattt ggacgattac atgatagact aagtaaaaag
    46921 aaacaaagag gtgtttacaa tggggttgaa atttgaacgt acagataatt tagataaact
    46981 tttcgaatca tttgctgtcg atccggaaaa aacagttgca ccaaaagaag agatcgatcg
    47041 atttctaaaa ccacagccac aaaatgaaaa gaaaaaagac aagaaacaaa agtaagttaa
    47101 gctcaaaaaa agacttacca gccaaacttt tatgaaagag tttgactggt aagtcttttt
    47161 tattcttttt atgattcaat tgcttgttcg attgctgccc acgcttcttc ttttccctgt
    47221 tttgtaacgg aagaaaagag aataaacgtg tcattgcgat caaaatccaa cgcttttttg
    47281 atttcgctct catgcttgtt ccatttacca cgagggatct tatctgcctt agtggcaaca
    47341 acaattacag ggatatcata atacttcaaa aattgataca tctggatatc ttcttgagaa
    47401 ggcttatgac gcaaatctac taaagaaacg accgcacgta gctggtctct agtcgttaaa
    47461 tatgtctcga tcatctttcc ccattttgct cgctcagttt tggatacttt tgcataccca
    47521 tatcccggaa catcgacaaa atgtaatgcg ttctcgatca aataaaaatt caatgtctgg
    47581 gtctttcctg gcttaccgga agtacgggct aaattctttc gatcaatcaa tgtgttgata
    47641 aaagaagatt tccctacatt tgatcgtcct gccagagcta tttctggcaa ttgtgtatct
    47701 ggatattgtt ttggtgccac tgcacttatg ataatttcag cttgatggac tttcatggtc
    47761 tctctcctta acaaggtgat gaataaagca agcggctgtc gcaaaagcca aaatgacttt
    47821 tacggcagcc tcttttcatc aacccgcttt tttctctgaa tctttataga ttactgttgg
    47881 ctcaccagac aattcagctg cttcttttgt gataatcact ttttcgatgt tttcatttga
    47941 agggatatca aacatcacat ccatcatgat ttcttcaatg atggaacgaa gtcctcgagc
    48001 accggtattg cgttcgattg cttttttcgc aatggctctc aacgcttcag gttcgaattc
    48061 aagttcggta ttatctagag aaagaagttt ttgatattgt ttgactagcg catttttcgg
    48121 ctcagtcaag atccgtacca gatcatctgt tgtcaatttc tctaaagctg ccataacagg
    48181 taatcgtccg ataaactcag ggatcaatcc gaattttagt aaatcttcag gaatgatatg
    48241 ctgcatgaca ctttcgtctt cagaaagttt ttggttttgt gtaccaaatc caatcgtctt
    48301 ttcgcccata cgattcttca caatcgtttc gatgccatca aatgcaccac ctacgatgaa
    48361 caatatgttc gtcgtatcaa tctgaataaa ttcttgatgt ggatgtttac gtccgccctg
    48421 aggtggcaca cttgcaacag ttccttctag aattttcagc aaggcttgtt ggacaccttc
    48481 gccggaaacg tcacgagtaa tagatacatt ttcgctttta cgcgcaattt tatcgatctc
    48541 atcaatatag atgatccctt tttctgcaag ttccacattg taatcagcag attgaagcaa
    48601 tttaagcaaa atgttttcta catcttcccc cacataacca gcttctgtca aacttgtcgc
    48661 atctgcgatt gcaaaaggta cattcaatgt ttttgctaat gtttgtgcta aaaatgtttt
    48721 accagaccct gtaggtccga tcaagcagat attgcttttt tgcagttcaa catcatctgt
    48781 gttttcttgt gcttgctgat tgacacgttt atagtgattg tatacggcaa cagctaacga
    48841 gcgttttgcg cgatcttgtc caatcacgta gttattcaat acttcaagaa tttctagtgg
    48901 tttagggact tcagtgaatt cgcgtacagc ttcttcataa aattcttcat caatgatttc
    48961 tttacatagg tcgatacatt cattacagat gtagacccct ggaccagcta cgatcttttt
    49021 cacttcttct tgggttttcc cgcaaaaaga acagcgtacg gtctcattgc tacttggatt
    49081 gtcatacatg gtcttcaccc cttaacttgc aaaaagtatg caagaaacta tcaaagactt
    49141 tacagtcttt tactatcata acatagttta gtaaaaagaa aaagtgttta acaaaggatc
    49201 aactgaccta tacctttgca ttttttactc ttaacaaaaa ataaggaact gtgacaaaat
    49261 cgtgtcacag tcccttattt cgaataaaca attttctaga agtagcttct tcagaaatga
    49321 tttattactt gatttctatt ctttcgcttc tgtcgctgta cctgtgatca attcgattgc
    49381 ttttttcatt gtcacatcat gttccagcat atcttctgtc aacacgcgtt tgatttgttc
    49441 gattggcatg ttgtatgttt ctgataattc ttggatttct gcattgattt cttcttctgt
    49501 tgcttgaaga ttttcagcag ctgcaattgc ttcgatcaca agatttgttc ttgtgcgcat
    49561 ttctgcttca ccttcgaatt gtttatgcaa gtcttcttct gtagaaccag ttaattgata
    49621 gtacatttca ggagagatac cttgacgttg catgttgttc aagaattcgt ccattgaacg
    49681 atgtacttca tcatgaacca tcacgtgtgg caattcaacg atttctgcgt tttcaacagc
    49741 catacggatc gctgcttcat cttttgcatc gtccgctgct ttttcttttg tttctactaa
    49801 ttctttgcga tatttttctt tcaattcgtc taatgttgat acttcgtcat caacatcttt
    49861 tgcgaattcg tcatctaatt caggcacttc ttttgctttt acttcatgga ttgtcacttt
    49921 gaagactgct tcttttccag caagatcttc tgcttgataa tcttctggga aagttacatt
    49981 tacatctaga ctttcacctg atttatgacc aactaattga tcttcaaaac ctggaatgaa
    50041 tgagcctgaa cctaattcaa gtgagtaatt ttcgcctttt ccgccatcaa acgcttcgcc
    50101 atctacaaag ccttcgaagt cgattactac tgtatcgcca ttttcagctg cagcatcttc
    50161 tttgataact aattcagctt gtgcttcttg ttcacgtttg atacgtgcat ccacgtcagc
    50221 atctgttact tcacggtctt gtttttcaac aactagatct ttatattgtc ctaattttac
    50281 ttctggtttc actgttactt cagcagtgat tacccagtct tgaccttttt ccatgctttc
    50341 aacatcgatt tttggttgag aaacaggatc gatgccagct tctttgacag cagcttcata
    50401 agcttctggt aatacagcat tcaatgcatc ttcatataaa gcttcttcac cgtacatacg
    50461 gttgaatact tgacgagaaa ctttcccttt acggaatcca ggtacgttca agcttttttt
    50521 cactttatta aacgcagttg ttaatccttt ttggatttcg tcttgcgcaa tcgtaaattt
    50581 taaaacacca tcattcgtgc cttttttttc aaatgttgca gtcatttaat tccctccgag
    50641 tttagaaatt cattttatac atcaaaaacg tttcatgcat acctttaaat agtacactaa
    50701 ccctttcaaa ttgtaaacat attgaatgga tagaatcctt gtttttcata acatttttat
    50761 ctttttcact gataaaatct aatggaagaa gatcatttgc tgcaaaattt tttctctttt
    50821 ttttaaaaaa tcttgttttt catcttctgt accttcatac atcgtttttc ctgtacacgc
    50881 ctctacagtc agttgaaacc agcgcttcgg gtcttcgatg tatgtatcat agacaggata
    50941 aacaattgca gattgcaacg tgaattcttc ttttaacacg gaactaagca gcgcatcttc
    51001 attcccgtaa cgatctgaaa atacacataa ttgctggtaa cttgcctgct gctgcggctt
    51061 tggcagatct tttggtatga ctttgactaa cttttcttca atcgtcaaat agaaaacagg
    51121 atggcattcg ttcaattgcc taagtgtctc cagtaatttt gcccttgcta aaatatggac
    51181 ctctggcgat acaataagtt tactcgctgt ttggataaac tcatgataag gaagctgttc
    51241 gatctcatca atagacgcaa gctgacgtgc aggttccaac gaaggaagtt cctccgattt
    51301 attgtggagt gccttgatct tctgcctctc catttgtccg gataattctt ctgcaaattg
    51361 gattttttca atgacccgct gctcgaaagc ttcataaaaa tctgtacttg ctatcaattt
    51421 tctagcatat aaaaaatctt gtgtactgat taacaagtca aaataaaatt cagcaaactc
    51481 ctcattttga agatactgtt tttcatgcaa gagtgcttgc ttcaatgctt cttttttctc
    51541 gtccaactcg taaagacaaa aaacaagcag gctattagcc tcaaagcttt ctttcaactc
    51601 gtaagctcgt tgaaataatt cttttgcctg ctgatattgc tggtttgcta gtgcttgttg
    51661 tgccactcgc attaaattct catatttttc tggaaaatca atcggcttca cgcagtgtcc
    51721 tcctcttgct catattcagc tcatgttcaa tagtagcaaa acaaaaaaag catgacaatt
    51781 aaaaataact gaagcaccct ctaaaagcta gattttagat ctgactttta gaaggtgctt
    51841 tcaactgtta ctgcttttta ttgctcatca gctgttgact tcctggttga acgctttgat
    51901 cagattgatc gtaacaacac ttgtattagt gaaaaaatcg cccagatgtt gctttttctg
    51961 attaaagact gctggaagat accctaacat cagcaaagga aattttagaa tgaaccggca
    52021 agcaccttct ctaactaaaa cggttgacca gctaagttct tcttcatcaa agctgatgac
    52081 tcgaatacca aagatcattt tcccaatcgt ttggccatga ttgaatttcg tcaataaaat
    52141 aaaatacgct agataaataa gtaaggctgt gaatccgtag agactcagat aagaatctga
    52201 aaaattaagc caacctagat ttttggcaat accaagtgtt aaacgcgtaa tagctccgat
    52261 acacaaaaga tcgaccaaga aggcaaacat tcggataaaa aaacctgcat agaaatactt
    52321 aggaaaatca tttgcttttg gcggttcatt tcttgtgtcc gtatactttt tccatttacg
    52381 ttgtttcttt ttgatttctt cgggcgtcaa aggctctctt tcaaaagagc tcagtacctt
    52441 ttgggaaaag ttggagtcag gtcctacagc aggctgctgt tgcttatctt tttcttgttc
    52501 cttattttca tttgaaagct ctgacatgct tactcacctc cgtaataata cattgctttt
    52561 ggtgattccg ctgtccctag attctcaaga acggataaga tctgactagt ctcagaaggc
    52621 ttcaatccct ggaattctgc tagtttactt cctaaccatg tactagcaaa cccagatggc
    52681 gtggaagaat attctactag ttccgcatct tcaagctgtt gttcctttcg tatagctttt
    52741 aaagcatctt caggaaatcc caattcatcg actaaaccta cttcttttgc ttggacacca
    52801 tcataaatcc ttccatcagc aatttttttg acttcttctt cagatttatt ccgtccctcg
    52861 ctgacgattg ctacaaaacg actatatgcg ctgtcgatat atgcttgaag caccgcttct
    52921 tcttcttttg tctctggtcg cgttcctgaa cccatatctt tcaacgctcc gcttttaact
    52981 gttgtatctt ccacacccag tttttctaga agtcctgaat aattcaatcc tgacatgatc
    53041 acaccaattg agccggtcac tgtttcttct gttgcaaaga ttttatctgc ttgagcagaa
    53101 atatagtagc caccgctagc tgccatgttt ttcatgctga catacattgg gatatcatgt
    53161 tctttacgaa tcgtatccaa tttcttagcg atttccgcac tttcgtagac accgccacca
    53221 ggagaattta cttctaaaaa gatacctttg actgttggat cttcttcaat cgccttcagt
    53281 tgttccataa aatttttatg attgtaacct tcagaagcaa ataaacctga agaccctccc
    53341 gcagcaatcg ttccatctac tgataatttt acgatttttt gcgtactgtc tccctcttcc
    53401 aacacatttg gagctagttc ttcatcccca tataaccaac tgttcaatcc ctcaatttga
    53461 tcgttttcct gaggcttagt caaactagct gaaactaacg aaaccacaaa taatcctgca
    53521 gcaatcagaa ctgctgccca acgtctttta ttcattcttt ctcctttcta atacaagttt
    53581 tttatgacga tcacagtttc tgactgctca gaagtgtcga accgctctgc tttttcttcc
    53641 cgcgcatacc cggaaaacga ctcttttgtt aattcatctt ctaccgtaaa ctcacgagct
    53701 tgagcaactt ccaaggcatc aggtgcgatt tcttggatgc tatcccagcc agtatctaaa
    53761 aatattttcg tatcatctga tacaccagct gatgcttcga atttggacaa ttgttttttt
    53821 aacattccca gtgtcatgat ctcaattttt gaatccattt ttactcctcc tctatccgat
    53881 tactttctat tgtaccgaac tcctgaaaaa gatgatagat aaaaacccat tattttttga
    53941 cataaaaagg acatttgaca aagaatttga tatataccaa tttatttttt attctttttt
    54001 tgttgacaag aacattcgca aaaggttatg attttaaatg tttccagata aaaaacaaaa
    54061 aggaggatag ttataccttt taggatagcg ccaggtctgt gtaaacagat gacgaggaga
    54121 gggtttatcg aaagattcgg cggatggccc tagggactgc actctacaga aaatccacaa
    54181 aaaatgtctg caaagataaa acaaaaggat tttcctgcag ctggaagcat tcatacgaat
    54241 tcattataga aagtaggatg ttttttatgt gcggaattgt tggaatgatt ggattaaaaa
    54301 atgtaacacc aggattgatt gacggtctag aaaaattaga ataccgtggt tatgattcag
    54361 ctggtatctt tgtctcagat ggcacaactg attatctggt caaagcacaa ggcagaattc
    54421 aaaatttgaa aaacaaaata aatattgata ctacaggaaa tataggtatt ggtcatacac
    54481 gatgggcaac tcacgggcaa ccttctgaag aaaacgccca tccacatact tcccaaagtg
    54541 gacgcttcgt gctcgttcac aacggcgtaa tcgaaaactt tgaagaactt aaaattgctt
    54601 atttagcaaa tgaccacttt attggtgaga ccgatactga gattatcgct catctaattg
    54661 aaactttcgc gaaggatact acaacgcaag aggcgttttt aaaagctttg cgggtcatca
    54721 aaggttctta cgctttcgct ttgattgatc ggacagcacc tgatgttatt tacgtcgcta
    54781 aaaataaaag tccactatta atcggactcg gcgatggatt caatgtgatt gccagcgatg
    54841 caatggctat gcttgctcat acgaaggaat tcgttgagat tgaagatgaa gagatggtta
    54901 ccgtcacatc agaaaaagtt attattcaaa attttgcagg tgacatcgta gaacgttctt
    54961 cattcgaagc acaagtagat gcttcagata tcgagaaagg tacctatcct ttttatatgc
    55021 tgaaagaaat cgacgaacag cctattatca tgcgtcgtat cgctcaaaaa tacgtaacag
    55081 aagacaatcg tgtctctctt gatcaaaagc tggtcgatac attgtctgat agcgatcgaa
    55141 tctatatcgt tgcttgcgga actagttacc atgcggggtt agcaggtaaa cagactttgg
    55201 aaaaattgac acagattcct gttgaggttc atttagccag tgaatttggc tataacacac
    55261 ctcttctttc aaaaaaacca ttctttattt tcttgagtca aagtggcgaa acagcagaca
    55321 gccgccaagt gctggtaaaa atcaaccgtc tcggctaccc ttcacttacg attacaaatg
    55381 ttgctggttc tactctttcg cgggaagcat cctttacact gctattgcat gctggacccg
    55441 aaatcgcagt agcatcaacg aaagcttata ctgcacaaat agctgtacta actttattag
    55501 ccaaagctat cggtgataaa aaagaatttg aaacttctct tgtattcgat atcactcatg
    55561 aattgagttt agttgctaat gcgatggaat cggtcatcgc ccaaaaagat tatttagaag
    55621 agttggcaga agtatatctc agcgatacaa gaaatgcctt ttacattgga cgtggtgctg
    55681 actacaacgt ttctcttgaa gctgctttga aattgaaaga aatcagttat atccaggcag
    55741 aaggttttgc cgctggcgaa ttgaaacatg gaacgattgc tttgatcgaa gaaggaacac
    55801 cagtgatcgg aatcatcagt gaagaagtga ctggtgcgca tactcgagga aacctgaaag
    55861 aagtcgaaag ccgtggcgct aaaacactag ttattgtctc agagggatta gaaaaggaaa
    55921 gtgaccagct ggtgctacca actgtccacc cttatctaac tactctaacc acagtcgtac
    55981 cgactcaatt acttgcatat tacgcaacgc tattacgggg attcgacgtt gacaaaccac
    56041 gtaatttagc gaaatcagtg acagtagaat aataaaagaa caaggaccag tgtcagacat
    56101 ctgacactgg tccttgttct tttatttttt atgaaaatat ctcttaatcc agtccgattt
    56161 cttctttgac tactgccgcg attttatcga catagtaatt aactttctca tcagtaggcg
    56221 cttctgccat cactcgaagt aatggttctg taccggaagg acgaactagg attcttcctt
    56281 ctgatcccat ttcagcttct gcctcttcaa tcactttttt gattgccgga acatccatcg
    56341 caccgttttt atcacttacg cggatattca ctaatttttg cggatagatt gttacctctg
    56401 ctgcaagctc tgataatttc ttacctgtct gtttcatgat attcagtaac tgaattcctg
    56461 aaagcatccc atctccagtt gtgttaaaat ctaagaaaat catgtgccca gactgctcgc
    56521 cgccaaagtt ataatcgttt ttgcgcattt cttctacaac gtaacgatca ccaacttgtg
    56581 taacgacatc tttcaaaccg attccttcca ccgctttgtg gaatcctaag ttactcatga
    56641 cagtcgtaac gatcgtatct tttttcaatc gatttttttc tgccaagtat ttagcacaga
    56701 taaacatgat tttatcaccg tcgacaatgt ttccaagttc atccaccgca ataatacgat
    56761 cgccatcgcc atcgaaagct agtccagcat ctgctccttt ttcaacgacc atctcagcta
    56821 aacgttctgg atgggtagag cctacgccgt cattgatatt taacccgtct ggatttgtgc
    56881 ccattgtata aaaatctgtt tcaagatcgg caaacaaacg attaacactt gttgaagttg
    56941 ctccgtttgc tgcatcgaca caaatggtca aaccggaaag gtcaccgttg atcgtttgta
    57001 caaggaattg agaatatttc aataagcctt ctggaaattc ttctacagtt cccaatcctt
    57061 cagctgatgg acgaggcaat tcatccactt cagcatctaa taatgcttca atttctagct
    57121 cttggtcatc tactagttta aaaccatctg caccgaaaaa tttgattcca ttatcttgtg
    57181 ctggattatg agaagcagaa atcatgaccc ctgcgcttgc tttttgcaaa cgtgtcagat
    57241 aagcaacacc tggtgtagag atcacaccta gctggaacac ttcgataccc accgataaaa
    57301 gtcctgaaat cagcgcttct tctaacattt gacctgagat tcttgtatcc cgccctacta
    57361 acacgcgtgg gcgttttcct tcttcttcat gctggcttag gacataacct ccgcaccgtc
    57421 ccagcttaaa tgcaagttct ggcgtaagtt ccttgttggc ttcaccgcgt acgccgtctg
    57481 ttccaaaata tttacccata gttgtaaaat tactccttta acttaatttt ctgaggaatt
    57541 tgttctgctc atttgtgttg attcgcttgt ttctgatgtt tcttctgtac ttgtggtcga
    57601 gctgcttgtc ccagattccg aactggaaga aatggttgtt gtcgatggtt ccgttcctga
    57661 atcagcagat gaaatacttc tactcgtact tgtattttct tgtgtccgac tgctcgtcga
    57721 agcagagcta ctgcttgacg tttgcaacac gggtgtaatc tccacactaa ctgattcggg
    57781 cttgatactt aacccctctt ctactggtat ttcaatttta cgagttgttc gatgattgat
    57841 acctgagaca ttgatcggta cagcaatact gtcaccgatc gatgcaagca aatcgctaga
    57901 gccgcttact tctgcttcca gttttgaaaa actgaaatca tagctttcta ccccggcagc
    57961 tggtgttcct tgctgtatcc cataaagtcg taccgacttg cttggcttga tcgaatcagc
    58021 tctcactgtt acttgcactg gatcagagac gatacttaat acttcccctg ctgcattcag
    58081 cgcctgtatt gggacctcac tattaatccc atcatctgtg accatcgaag cgtccacatc
    58141 agctactact cgtttgattt ctgccaatgt tttgtcacct gtagtgatag agactttttt
    58201 aggactaacg gaagtatcat caagttcata tccagatggt gtgaacgttg aaggaatcaa
    58261 cgtttccaca tcgaaatctt ttattacctt tttctcaacc gttactgtaa tagtcgatgg
    58321 ttcgatcttt gctgtgacag cactgctcaa attttgaatt tttagcggaa cttcatgtgt
    58381 tccttcgccc agcttagtga tatctgctgt gacgcggaac atccgtgtat cttcgtttgc
    58441 ctctgcattc aactgaactc ggtttgcact ggaaagtttg acagtcactg cattttcata
    58501 tccatggata tagtagcgct ttgaatcata agaaatcttg atcggcaccc ccgtaactgt
    58561 ttgattatac gcttcatttc cagaaagcgt atttggaacg gactggccat ttgcattgaa
    58621 gtacagcgca atactgaaca gcaatgcaat aaaactgtaa agaatgcttg tccgtttctc
    58681 ttttgagatc atttgcgatc ccctccctta gtcataccgt ctaggaaaga ttgaatcacg
    58741 tttttcttgt tttttgtttt ctcttcagca attagttctc tacgcaagat tgccaaatat
    58801 tcttgctggg ataagccagc catcagttca ttatctaacg tgatgctgac gtccccggtt
    58861 tcttcagaga caacgatggt aatcgcgtca ctgacttcgc ttataccgac agctgcccgg
    58921 tgtcgagtcc cgaattcttt agggatcaac atactttctg atagaggaag ataggcgctt
    58981 gctactgcga tctttccttc ttttacaata actgcaccgt catgcagcgg tgtattagga
    59041 ataaagatat tgatcagcag ctccccagtg atatctgcat ctaaagcaat ccctgtctca
    59101 atatactcat ctaatccagt atgacgttcg atagtgatca acgcaccgat ttttcgcttg
    59161 gacatatatt ggatggcttt gtcaaaagac aagatcatct tctcatcttc ctgctgttcg
    59221 ctttttgatt gtttgaaaag cgaactgcgt ccaagatgtt ccagtcctct ccggatttcc
    59281 ggctgaaaaa tcacgatagc agctatcacc ccgtacgtaa tgacttggtt catcagccaa
    59341 gacaaggtgt gcagaccaat gatttccgct acgattcgga taacaataaa aatagctaca
    59401 cctttcagaa gctgaatcgc ttttgtgcct cgcagcagtt ggatcagttt gtatacaaga
    59461 taccagacaa ccagaatatc aagtatgttc accaaaaaat tcagagagaa caagtcaccg
    59521 gaaaacattt gctgccaata gtccaaatta aaaagttccc cgatttgaaa tgacataaaa
    59581 agcctctctt ctcttttgag atttcctctc tagtatatca tatgttttaa ttttagaggc
    59641 tgatatgctc gtatttttct tgagaaaaac gcataatttt caagaaaatt gcaagtatgc
    59701 gttttatttt tctctgcaat aaatattttt aaacttcaga gaattattca ttatattttt
    59761 tctgttattt ttcctttttt tcttttgtag cttgttccat ttttttacgt cgttctgctc
    59821 tggcagctct tctagcagct cttctttctt ctgcttcaag gtccgtcgtt ttttcttttc
    59881 tagtcttaac gggtacatcg atagattcct ctggttcaag tcgtttcttg attttttcta
    59941 attctcccgt cagtgcagca tagttgtaaa aacgtgtttt agcgtcgtgg acagtctttt
    60001 caaataaagc ttttgcaaga tttggatttg cgttatctag cgaggaaaag cgaacttgtt
    60061 tcctcatgaa atcaggcata gcagagaaat caggtttttt gtagtccaaa gtcattgggt
    60121 tcttgttttt ttcgaccatt gtagggttat atcgatataa tgaccaataa ccagaagaaa
    60181 cagcttcttt tgcttcttta agcgattctt tcattccgcc aagcaagcca tgtgtgatac
    60241 aaggcgtata ggcaataatc agagaaggac caggatactt ttctgcttct tcaaatgcct
    60301 tgatcgtttg catttgattg gcacctgaag caatttgcgc cacataaaca tttccatatg
    60361 tcatcgccat cattcctaga tctttctttg aagcatactt tccactagcc gaaaatttag
    60421 cgattgccga agctggtgtt gcttttgagg tttgaccgcc agtattggaa tagacttcgt
    60481 tatccaaaac aaggatattc acgtctgctc cgctggcaag tacatgatcg atcccgccaa
    60541 aaccaatgtc gtaagcccat ccgtctccac caatcatcca ttggctgttt tttacgaata
    60601 gatcctttct cttgtatata tcctccagtt cagggaaaat tgatcgttcg ctttcgatcg
    60661 cagcttgcag ttttgctgct ctttgctgcg ttccttcact ctcaaacatg tgtactaacc
    60721 agtcttccat caattctcgt agttcttctg atacttttgg caatacttct ttgattgaac
    60781 ttgccaaagc ctcgcgacga gtctgtgccg ctagaagcat accataacca aattcagcat
    60841 tatcttccaa taaagaattt gaccatgcag gcccttgacc acattcattg gtcgtataag
    60901 gagaagcagg agaagcaccg ccccaaatag aagaacagcc tgtcgcattc gcgatcatca
    60961 ttcgatcacc gaacatttgt gtcaataatt ttacataagg tgtttcgcca cagcctgagc
    61021 aagcgcccga gaattcaaga agcggttgat tgaattgtgt agacaagaca gtattctttc
    61081 ctttgactgg atttgctttt tgtcttaatg tcattgcaaa agcccaattc acagcttgtt
    61141 ctttctgttc ttcataaggc ttcatcacta aagctttttc ttttgcggga caagcctcta
    61201 cacacagacc acaacctgta cagtcttcca ctgaaacttg gatacggtat tttacgccgt
    61261 ctgcgcctcg catttctctc acgataaatc cttctggcgc ttcttccatt tcctcttcat
    61321 ctgctaaaaa tggacggatg gcagcatgag gacaaacaaa ggcacattca ttacacatcg
    61381 tacagcgatc actaatccac tcaggtactt ctaaagcgac gccccgcttt tccatagaag
    61441 ccgttcctaa aggcatttcg ccagttgtca ttccatttgc tacaagtgta ccaacagaga
    61501 gttgatttcc ttcctgtgca ttcactggtt caagtatttc ttgtatataa ccagtgttcc
    61561 ttttattgat tttttcaggc atctccaacg ttctccaatg gtctgggatc gcgacttgat
    61621 gaagcagttc ggtcgtccga tcgattgctt gtatgttttt ttctacgata gccattgatt
    61681 tatgtccata gcttttcagg gcttcttctt ttagcagcgg aagcacctca tcaaaaggaa
    61741 tgatgtcagc taatttaaaa aaagccgttt ccattgccgt attgatccgg cgtcccaaac
    61801 cgacttcttg ggccaatctc atggcattga tcgtatagaa tctaatgtta ttttcagcca
    61861 agtaacgttt taattttaag ggcagatgcg tttctaattg ttcgtcgctc caaagagtat
    61921 tcaataaaaa aatgccacct ggcttcaatc ctttgaccaa gtcatagcta tgcaggtaag
    61981 ctggtgtgtg acaggcaaca aggtccgcat gctcgacaag atacgctgaa cgtatcggcg
    62041 tatctccaaa acgaagatga gagacagtca gaccgccaga ttttttcgaa tcataataaa
    62101 aatagccttg cgcatattta tcggtgtgat ctccaataat cttgatagct gatttatttg
    62161 ctccaactgt tccgtcagat ccaaatcccc aaaacttggc ctggaatgtt tgcggttctg
    62221 tcaaatccag actttccttt ggttccaaag attgataagt cacatcatcc acgatcccta
    62281 tggtaaaccg ttttttgcgt atgcttgggt cttttttcaa ttcatcaaaa actgctgaga
    62341 tttggtcggg agtcacatct ttagacccca atccgtagcg accaccaata acagccgggc
    62401 gaagttcaga atcatataac gcactttgaa cgtctaataa gagaggttct ccacctgccc
    62461 ctggttcttt acttcgatca agaacagcaa ttgtttttac tgtatgaggc aacttttgca
    62521 cgaatgtttc cactggaaaa ggacgataaa gatggatatt caaaaaacct gttttccgtc
    62581 cgtttgcatt caagtaatca atcgtttgtt caatcgtctg tgcaactgat cccatagaaa
    62641 caatgacttc ttccgcatcg tctgctccgt aataagtcac caagtcataa gaagttcctc
    62701 gtaatcggtt gatctctttc atatattttt gaacgatttc aggtaattta tcataatagg
    62761 ggttgatcgt ttcccgttgt tgaaaatgga tgtctggatt ctggttcgta ccactaacag
    62821 tcggatgatt gggattcatc cctcgtttac gaaatgaagc taatttctcc tgattgatca
    62881 gaggagctaa ttcttcatat tccaaaactt cgattttttg aatctcgtga ctcgtacgga
    62941 atccatcaaa gaaattcaaa aaagggactt ctccttcaat agaagcaaga tgggcaacag
    63001 gtgctaaatc catcacttct tgtacactgc tttcagctaa catagcaaat cctgtttgtc
    63061 ttgcagccat cacgtctccg tgatctccga aaatattcaa cgcatttgtc gtcactgccc
    63121 gactagcaac gtggaaaaca cttggcagta gttccccagc aatcttgtac atattcggta
    63181 tcatcaaaag cagcccttgt gaagcggtat atgtagttgt cagtgcacca gttttcaatg
    63241 atccatggac cacaccagct gctcctgctt cggattgcat ttctactact tttactggtt
    63301 gaccgaatac gttttctttt cctgctgctg accattgatc tactaattca gccatggtcg
    63361 aactgggtgt tattggatat attgcagcaa tctcggtaaa tgcataagaa atatatgcgg
    63421 ctgccgtatt gccatccatt gttttcattt ttcgcatgat tcatcatcct tgttcatatt
    63481 ctctcttatt atctgttcca ttataacgta aaacatgcca tttttttctt ttatttataa
    63541 aaatatttta catgtatcta tttgaattgg tcatctaatt tttagacaaa cagtctaaat
    63601 cgttgccttt ttttgtcaga catgtataaa ctataagcga aagaggtgtt ttaacatgga
    63661 tatatcagtg attgatgcaa cgaaaacaaa tacacaaaaa ggaatccttt atggttcatc
    63721 gaatgctcct aaaaaaatgg tcgaattcat caacttagct tgtccgtatt gccgacaatg
    63781 gtttgaagaa tcttatgagc tattagaaga agcagtccaa agcgggcaat tgcagcgagt
    63841 gatcaaattg ttcgataaag aaaaagaaag cttacagcga gggaacgtga tgcaccggta
    63901 tctcacaatc agcgatggcc aaaaagcgat caaagagatc aaacaaatat tcgatactca
    63961 agacgagtgg aaacatcttt ctctccaaga agtagcggat tttgcagtag ataaactaaa
    64021 gctgacagaa caaaaagacg aacagctgag ccaagctgtc atcaacgaag ctgagcaagc
    64081 acatattcga tttgttccaa ctgtcatctt gggtaaagaa atctttgatg aatctatttc
    64141 aatagaggaa ttaaaagaat tgatccaaac aaaataaaaa aataaggagc gtg
//
LOCUS       DACGLE010000008        56389 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0534
            SAMEA3304449-rid9560453.denovo.008, whole genome shotgun sequence.
ACCESSION   DACGLE010000008 DACGLE010000000
VERSION     DACGLE010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304449
            Sequence Read Archive: ERR985064
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 56389)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 56389)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702225.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702225.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 20:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,045
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 27 of 67
            Pseudo Genes (incomplete)         :: 25 of 67
            Pseudo Genes (internal stop)      :: 19 of 67
            Pseudo Genes (multiple problems)  :: 4 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..56389
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304449-rid9560453.denovo.008"
                     /strain="VREN0534"
                     /db_xref="taxon:1352"
     gene            330..1175
                     /locus_tag="IXT20_000607"
     CDS             330..1175
                     /locus_tag="IXT20_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAQ4859081.1"
                     /translation="MDEMKLIGIDLDGTLLNSDQSISQKNARTMKHLPEDCFPFICSG
                     REVEDINNILKKSGLRIPAVGLNGSVGFDGEKKIFESSFDKNDVKKIDSVLSKFPTKI
                     YTNLGSYESQNYKNKMKKIFHEVGSEFSIKELNYELEYEKSICSIPYNNIEEIIEKDA
                     ISIYKFFIFIPNRQLKANIFNHLKKMLNVSVTESAPVNIEIVPQNVSKGVVFDRLESI
                     YNLKKPLRIAIGDSLNDLSMFESADISFAMGNSHQEIMKKADYVTVSNDKDGVSQAFE
                     RILTI"
     gene            1456..2676
                     /locus_tag="IXT20_000608"
     CDS             1456..2676
                     /locus_tag="IXT20_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair exonuclease"
                     /protein_id="HAQ4859082.1"
                     /translation="MVKFIHAADLHMDRSFEGLTTLDKTVQEKLLKTNLTVLSNIIEQ
                     AIINNVDFVLLAGDNFHQNRPSLKIQKHFSEQMKRLEKNHIPVFIIFGNHDFYQKERY
                     WFSFPKNVHLFTSETVETKKITIKSGETVSLSGFSYRQPWIQKDKVMEFPSRELTDYH
                     IGLYHGEPGVSQKGNYAPFQPSKMQEKGYDYWALGHIHVPTVLNEKQTIVYPGAPQGH
                     TKKEQASTSILLVELSKGSCQIHPIKVAEVYWKTKEISLRKARTTKEVIVHIRQQLSK
                     VEDGFSLIEIKLKDYDHLNTDVLERIQSGELLDYLLEEFSDRINDFFIWRISLIENTF
                     YTKTPLAASAKLMEQLFQYYQTPQDFQQILNEVYSHQEAARILSELPEYQEETLEKAK
                     QLLNQDFLFEEDQE"
     gene            2673..5393
                     /locus_tag="IXT20_000609"
     CDS             2673..5393
                     /locus_tag="IXT20_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAQ4859083.1"
                     /translation="MKILRIEIAAFGKWRQKSFDFYSGNQLIYGGNEAGKSTIYQFIQ
                     AILFGFPSKGRKKKDYTPKDGSAYGGKIWLKHPVYGEFAVERYKQQNRGKSKVWLGDQ
                     VGSDELLEKLIAPLTKELFQQVFTFQQEQLMELDHLREKELHDALISLGITGSSQVFQ
                     KRQEYYQKAQQLFKKKGQKLLINQKLNEWKELQEKIHQKQEQEAYFGKLIQQEQEYES
                     QQQQLLAQLKEANKQFSLARQQELNFSLYEEWQSLKKNSTTGLPDEQLLLDLESFSKR
                     YQQTNERLEELEATLEKHSGMDQQSARYYFYLEQEQELKDLQQLQFTAEQLNEEWQRL
                     QIETRETNSELKILEQRWHWQKNPLPEEFLYEEEWKHLLAEERRIADLESQISVRLQI
                     AKEQQEMIEAEVNDLEETYPALLQNQSEIAPVSSSKPYMLMAGGAIIIAGIFVPMPLK
                     VILLLIGAGMIGYFFYKRQPKIKTKNDFSEIKGMWQEKLGQLDLALAQLAEAEEKQKR
                     TGEEKQILQQEVYRFADHRHLGKMDTLKLMKEYGQEVIDYQKLYGVFERKHKREQEIQ
                     EKLRQIDQKFEFVKEWLPIQDKTISEKMVLLSRFIQEMEQMKFARSYQQNTLLKQEIN
                     QLKKKQKVWIQEYSEQLTQAGLSYPSEVPVFLQKAKQAEQQMKRYNELSEMLTPLFPE
                     ELTKEKLSVRLRQLKEKQSQLQQEEKEASENRQRLQLQVEELQRDGTLDELYQQASRQ
                     KAKLQELLEEWGALEIAGTFLGDLSTEMSERQLPQLLRAAGDYLGILTKGNYQKILLK
                     NDTLTLTDGYTEFPIYELSTGTKDQLIMAMRFAYLSLEANRSICPIIIDDGWLHYDHQ
                     RKYQLAELLAKFGEKYQIICFSSDKEMVSYYHEMNQIVKQLEGANNEKNS"
     gene            5377..6321
                     /locus_tag="IXT20_000610"
     CDS             5377..6321
                     /locus_tag="IXT20_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAQ4859084.1"
                     /translation="MKKIRDLVIDEQFEQFVLIKSADVRIAKNGKKFIAFTFQDTSGT
                     IDGKYWDASEEEINKFTAGTVVFLNGKREVYQGNPQVKILHMRLTRVDEPGEPSLYME
                     RAPLKKEEMEEEINQLLFEITNAHWNRIVRYLLNKYQKQFFEFPAAKRNHHAFASGLA
                     YHTLTMLHLGKSIADEYPELNRALLYAGIILHDLGKVIELSGPMSTEYTLAGNLIGHL
                     VLIDEEITKACLALKISEADEDVLVLRHMVLAHHGLLEYGSPVRPRIMEAEILHQIDN
                     IDASMQMMLTSIRQTEPGKYTDRIFGMDNRSFYVPKDL"
     gene            complement(6390..7400)
                     /locus_tag="IXT20_000611"
     CDS             complement(6390..7400)
                     /locus_tag="IXT20_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAQ4859085.1"
                     /translation="MKKKSIILAATSALAVLTLAACSGDTNKDIATMKGGTITVSDFY
                     DEAKLESSNQSLVQRMIIYKVFNNKYGDKVTDKQVDAEYDKQAKSLGDTFESQLEAAG
                     YTKDTYKEYIRNNLAFEAGLKAHVDITDDDLKTAWKSFHPEVEAQIIKLSSEDEAKDV
                     KKSADDGDDFSKLAKDKSTDTETKEDGGKVKFDSTTTTIPAEVKEAAFKLKDGEISDV
                     ITTTNPTSYATEYYVVKMVKNQNKGNDMDKYKDQLKDIATETKLSDNAFTTKVIGEEL
                     KDANVKIKDDAFENVLSAFTTTSSSTKDSSETTASTKSSDTKSTDSTKESSTEETTDS
                     SK"
     gene            complement(7580..7924)
                     /locus_tag="IXT20_000612"
     CDS             complement(7580..7924)
                     /locus_tag="IXT20_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HAQ4859086.1"
                     /translation="MIKNFFKGLLFGAAAGTVGGLLLAPRSGNETRKKLIEELEEATT
                     LTNDLNNSLNHFKSALIETKETAESVIPAFQEAIKKDIEDFKFQAEPRITQINEQVEK
                     LKADLPEYPEQI"
     gene            complement(7917..8351)
                     /locus_tag="IXT20_000613"
     CDS             complement(7917..8351)
                     /locus_tag="IXT20_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HAQ4859087.1"
                     /translation="MENCIFCKIINQEIPSYKIYEDDKVYAFLDISQATKGHTLVVPK
                     RHVADIFEYDPELAGEVFSRVPKIAQALEKAFPEMKGLNILNNNRELAYQSVFHSHIH
                     LVPRYSKEDDFSIHFVNHQDSYGSEELKAIQETIVKQVSCDD"
     gene            8489..9226
                     /locus_tag="IXT20_000614"
     CDS             8489..9226
                     /locus_tag="IXT20_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ4859088.1"
                     /translation="MSLIIDHVTGGYGHLPVLKDISFDVQPGEIVGLIGLNGAGKSTT
                     IKHIIGLLEASKGTIEINHLSLKKNTEEYRKQIGYIPETPSLYEELTLREHIEVTAMA
                     YDIPMDIAMKRAEKLLETFRLSNRLDWFPANFSKGMKQKVMILCAFLIEPSLYIIDEP
                     FLGLDPLAINALLELMVEMKKEGAGILMSTHILATAEKYCDRFVVLHNGEIRADGSLE
                     MLRKEFQLPDSSLDEIYIALTKEQAHE"
     gene            9219..10430
                     /locus_tag="IXT20_000615"
     CDS             9219..10430
                     /locus_tag="IXT20_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ4859089.1"
                     /translation="MSSFFQKRLARHQKKMMKYMRYVLNDHFVLVCLFLIGGVGLYYS
                     NWLKTLQPPFAIGGLIISVFWMLCLFVGKMATFAEAADIVFLLPKEKEMQTYLERAFR
                     YSCIFPLIFLILACGLAMPLVVVSTEQSFSMFFVYLIILWSLKFSHLQIKRAELFRLD
                     QQVIRYWKLGWFFSSLIFLFLSFYWLIWAGIAGALVQASLYYWFLWKQQEKGLDWEKM
                     VHVEEHRLHQIYQFINLFTDVPEITAKIKRRRYLDPILTHIKRESKNTYLYLFARRLA
                     RGSDFSGLYFRLVIIGGLLIAGVVDFYFSLIIGFLFLYLIGFQLLPLANQFRYMVLVQ
                     LYPIHKNQKKQAIQYLLTWVLIIASVIFGLIAAVVLDGQQRWVPLAAYLIVTMLFVKL
                     YVPTRLKKMSD"
     gene            10637..11416
                     /locus_tag="IXT20_000616"
     CDS             10637..11416
                     /locus_tag="IXT20_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HAQ4859090.1"
                     /translation="MEFQLDQEWRLRPIKGDTGKTYIGMRDEDKVFIKRNTTPMLAAL
                     SREGITPKLVWTKRTGNGDTLTAQEWLEGRLLTPNEIGQRNDVIDVLYHLHHSNSLKT
                     MLTRIGGRVMTPEKMLQKYEKELPEELQKNQFLQIVHRFLREQIPSIDKENLSVVHGD
                     VNHRNWLVCKNYLYLVDWDSIMFADPAVDIATILGNYVPLSSWNQWLISYGIRPTDEM
                     LEKFHWYAAMNLLQEITRYCKREDHRRMNEEILQLKRIFSG"
     gene            11486..12148
                     /gene="trmB"
                     /locus_tag="IXT20_000617"
     CDS             11486..12148
                     /gene="trmB"
                     /locus_tag="IXT20_000617"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HAQ4859091.1"
                     /translation="MRVRNRPGAAEMLAAHPNFVISDPTLWKGKWNELFENDHPIHIE
                     IGMGKGQFITGMAKAHPEINYIGVEMQVSVVSIALDKLIEQPLPNLKLLHVDGSALTE
                     YFADSEVDQIYLNFSDPWPKKRHEKRRLTYKTFLAVDEQILRPNGEIHFKTDNQGLFE
                     YSLASFSQYGMILKQVWLDLHQSQFEGNIMTEYEEKFSSKGQRIYRVEAQFQDKTKKK
                     SL"
     gene            complement(12246..14000)
                     /gene="ade"
                     /locus_tag="IXT20_000618"
     CDS             complement(12246..14000)
                     /gene="ade"
                     /locus_tag="IXT20_000618"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HAQ4859092.1"
                     /translation="METTLRKYIEVASKKKPADLVIKHAKIVNVFTKEIMEKDVAICE
                     GMIVGIGEYEGKSVHNANGQYLVPGFIDGHVHIESSLLSPSEFSKVSLLHGVTTVVTD
                     PHEIGNIAGSTGLEFMIEDARQTLMNIFFMLPSCVPVTPFETNGANLNAKKLAPFLQK
                     PEVLGLAEVMNYQAVANNETDILEKIQLMHQHKKKIDGHAAGIGMEELNVYPAAGIRT
                     DHEATTAKEAKERLDLGMYLMVREGTVAKDLASLLPAITPENSRRCFFVTDDKLINDL
                     VKEGSIDHIVRQAIALGIDPLQAIQMATLNAAECFDLKTLGAIAPGYQADFFLTDDLT
                     KLPIHEVFYKGTLVVQSGQLQDHLFADHSNSYTCCLPKLNAQPLNPHSLELLLSSSQA
                     HVIEIIPNSLVTKDLVEKVDRQGERFIPSIEKDQLKIAVIERHHQTGNIGLGIVKGFQ
                     LKQGAIATTIAHDSHNLVVVGTNDEDMLYAANLLIQKGGGMTVVNKQQEIACVSLPIG
                     GIMTQEPFSIVYEQLNELITKAHQLGAAKTFDPFLTLSFLTLSVIPELKITDKGLFSF
                     SRFQLIPPCGKTDEINTF"
     gene            14132..15061
                     /gene="cpaF"
                     /locus_tag="IXT20_000619"
     CDS             14132..15061
                     /gene="cpaF"
                     /locus_tag="IXT20_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly complex ATPase component"
                     /protein_id="HAQ4859093.1"
                     /translation="MEIKRLSDQMISESFAKHAQDVYILPASEVFYIYFRYGKKRTFW
                     KKVSSADGQQLISRFKYLGQMDIGEKRKAQLGAFTYSLEKQEVRLRLSSVGDYLQRES
                     LVIRLLYGTFETPHRCFSSRDFATILQKTVRRGLYLFSGPVGSGKTSLMYRLALEEKL
                     QVITIEDPVEIEEPFFLQLQINPKIAQTYDTLLRLALRHRPDLLIIGEIRDESTAHAA
                     VRAALTGHRVFATVHARNLEGTLVRIHELTKKKEELSECLAGVIYQELLEDANHHPSV
                     LWSYQFMEEESVKKSWEESYEEAQENIWQSELF"
     gene            15018..16067
                     /locus_tag="IXT20_000620"
     CDS             15018..16067
                     /locus_tag="IXT20_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComGB"
                     /protein_id="HAQ4859094.1"
                     /translation="MKKLRKIFGSQNYSKGLTRSQQNQFAQTMGELLLSGFSLQESLY
                     LLLTIRSFPPVILANIQHQLKEGQAFSEVLSQIGYGAEQLVQIELAERHGNLPTTLLE
                     IAKQFRLVEGFRNDLKKLLAYPMLLLTFLLGILFSMRLFVLPQLIGSGMVMQEHWGIQ
                     LIQVFHWYVLLFILAVTLLALLVRYRLMKLSPVDQSEWISRQLLIGPIYSLYQSAYLS
                     LEIGKLFHEGFELRQIIVCLKETKQESLIQHLASKLIQGLEAGMSLAEQFQNYRFLSE
                     EFSKIVLQGEAKGSLGKELIFYSEWTRKQLFKRLHHWMHFIQPIIFLGVAALILLVYA
                     AVLLPVYGSIEEVLP"
     gene            16064..16342
                     /locus_tag="IXT20_000621"
     CDS             16064..16342
                     /locus_tag="IXT20_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HAQ4859095.1"
                     /translation="MKKRKISYYSGFTLIEMLIVLLIISVLVLLFVPNLSRYRNHVDQ
                     ESREAIIQLVDTQKELYALQNNGRVPTVEELLNEGYIKREHAEIYQRP"
     gene            16317..16787
                     /locus_tag="IXT20_000622"
     CDS             16317..16787
                     /locus_tag="IXT20_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HAQ4859096.1"
                     /translation="MQKSISGLEGKVLASGYTLFESLLLILLLTTILSFPVLAFSAWK
                     NELAVYQFFGQFEKRIYSTQKIAIVNQIQTGFYWNSEENQIIFHVPNPDKINWEVLDI
                     PDEITLKRHASITFAARTGNESSLKAYQFYWEEKERTITYQFQIGSGRYTKRIE"
     gene            16804..17106
                     /locus_tag="IXT20_000623"
     CDS             16804..17106
                     /locus_tag="IXT20_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4859097.1"
                     /translation="MESLTALMIASVCVYLVSFGQLFLLQAEKKSGEELLFIRMIYED
                     IKTQRMYHKRPERREEMIPYDIQIVDNNGVIEKVFVNMEKEAISIEKTSNSSIYAD"
     gene            17123..17494
                     /locus_tag="IXT20_000624"
     CDS             17123..17494
                     /locus_tag="IXT20_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComGF family competence protein"
                     /protein_id="HAQ4859098.1"
                     /translation="MILSSLLLTFNLLIQQTIQVSEFLQRKDQKEWLIFLAQLEEELK
                     SSHNVSVKNQQLHYTIEDKQYIIERYQAMIRKRRTSGGHQPMLTSITELQLLEKEHTI
                     SFYVHFENGEDGYAIWTKNDQ"
     gene            17466..17825
                     /locus_tag="IXT20_000625"
     CDS             17466..17825
                     /locus_tag="IXT20_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4859099.1"
                     /translation="MPYGQKTISRKCYKGSILFSVLLLFLLCTFLLMAALESYRLSMD
                     LNIRIKNYYIAKIMKQMALSEQSTLAESSEGVFYYTTGSVTYQWVQQSLFLEVEVSPF
                     IFRFTEEVKNDTDTSTE"
     gene            17997..19004
                     /locus_tag="IXT20_000626"
     CDS             17997..19004
                     /locus_tag="IXT20_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAQ4859100.1"
                     /translation="MLPEKMEQGFNQNLEAIQLLQNALGTSFLEAYVENAENLIDDYQ
                     VRVVDGVPTKETTQRITALYEELKKLSFEPEEWRRLSQLLLLKGSQTEHLQANHQLTP
                     DSIGFLFVFLIEQLYTNKKAPVKILDIAAGMGNLLLTVLLNLSNAGYQTEGIGVDIDD
                     TLLAVAASTSDLTQANVQYFHQDGLQELLIDPVDFAISDLPIGYYPNDEKAKEFLTST
                     EEGHSYAHHLLLEQSMKYVKPDGFGLFLMPSGFLETDQSEEIKKWFKEEGYLQGMIQL
                     PDELFRNKQSQKSILILQKKGPQAQQVKEVLLVKLASLKEPEKVTEFFNEFKNWKSSA
                     L"
     gene            19029..20213
                     /locus_tag="IXT20_000627"
     CDS             19029..20213
                     /locus_tag="IXT20_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HAQ4859101.1"
                     /translation="MSKTIAINAGSSSLKWQLYQMPEETVVAKGIVERIGLQDSIFTI
                     KYGDNQKFEQIMDIENHEVAVKMLLDQLIELQILSSYDEITGVGHRVVHGGETYGDSV
                     VIDEKVMDRIAELAEFAPLHNPANLMGIKAFKKILPDILSVAVFDTSFHSTMPKQNYL
                     YSLPVSYYEDFKVRKYGFHGTSHRYVSERAAKLLNKPIEELKIITCHLGNGVSITAVD
                     GGKSMDTSMGFTPLAGVTMGTRSGDIDPAVLPYLMEKLEIDIDEMINVLNKKSGLLGL
                     TGLSSDMRDLEKNYEKEHIRLAYDIFVDRIRKYIGGYVTTMNGVDAIVFTAGIGENDG
                     HVRSEIIKGMSWFGCEIDPALNELRGEEVDISTKESKVRVLVIPTDEELMIARDVERL
                     RG"
     gene            complement(20650..22233)
                     /locus_tag="IXT20_000628"
     CDS             complement(20650..22233)
                     /locus_tag="IXT20_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAQ4859102.1"
                     /translation="MIIQAISIKKTIGGNPLFEDLSCQIEAGEKIALVGINGTGKSTF
                     LQILTGREGVDSGVISRKKGLKIRTVEQELTVSEATVNHYLLHSAAEIQDLKQQMSRY
                     EALMTEPDTNLEKCLARYGELQHRFEELGGYVLEDRIAAILQGLGIPHRQEARLTELS
                     GGERVKVALAKILAADADLLLLDEPTNHLDLNSIRWLENYLQNTKKSVLVVSHDRQFL
                     DQVTTKTWELEEGALIEYPGNYSRFRVLKEARLAELTKNYELQQKEVQRLKVMIRRFR
                     QWAHEGDNESFFKKAKELERRLAKLTLVKPPPPPKNRLQSLSNGGKSGKEVFIIQNLH
                     QQYADQVLFKDSSFAVYRGDHLAIIGDNGAGKSTLLKLLLGEEKPYGGTIKLGSSLQI
                     GYLPQQLQFADPDSRLLAYAITLTGNEEVARRQLAKSGFYQSDVGKRIKDLSGGEKIR
                     LALLKLFLEKINVLILDEPTNHLDSYAREEIEEMLQDYQGTLLAVSHDRYFLQQHFQE
                     ALVIDQEQISRQPLGQLID"
     gene            complement(22811..22954)
                     /locus_tag="IXT20_000629"
     CDS             complement(22811..22954)
                     /locus_tag="IXT20_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HAQ4859103.1"
                     /translation="MEVSDESDTKKYEVSMADTDFIAKLKFYFGFGYREKASATFGKS
                     FKN"
     gene            complement(23177..23656)
                     /locus_tag="IXT20_000630"
     CDS             complement(23177..23656)
                     /locus_tag="IXT20_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HAQ4859104.1"
                     /translation="MLQQYKKIMVAVDGSDEAELAFKKAVNVAIRNNGELLLAHVIDT
                     RSFQTVSSFDGMLAEQATEMAKQTLADYESNAKKAGLNNVTSVVEYGSPKQIIAREIP
                     EDNQVDLIMLGATGLNAVERLFIGSVSEYVIRNAACDVLVVRTDLENQLPAKDHTKA"
     gene            complement(23729..24013)
                     /locus_tag="IXT20_000631"
     CDS             complement(23729..24013)
                     /locus_tag="IXT20_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4859105.1"
                     /translation="MIRILMIIATLVLLFVSYYLFNKQDIFFVLINKNDKNQGFLQFY
                     GAAYAVLGVMGILTAFFNQRFIALVFLLIVIIVSATFSIRFAKKIAEPKQ"
     gene            complement(24091..24285)
                     /gene="ssrS"
                     /locus_tag="IXT20_000632"
     ncRNA           complement(24091..24285)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="IXT20_000632"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(24357..25643)
                     /locus_tag="IXT20_000633"
     CDS             complement(24357..25643)
                     /locus_tag="IXT20_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HAQ4859106.1"
                     /translation="MQQPLAYRMRPRTIDEVVGQQHLVGEGKIIRRMVDARMLSSMIL
                     YGPPGTGKTSIASAIAGSTNYAFRMLNAATDSKKDLQVVAEEAKMSGTVILLLDEVHR
                     LDKTKQDFLLPHLESGRIILIGATTENPYITINPAIRSRTQIFEVKPLTEQDIQLAVE
                     HALRDKERGLGQQAIQLDEDALLHLSRATNGDLRSALNGLELATLSTPSDKEGRIHLT
                     LSIIEECVQRKALTHDKNGDAHYDVISAFQKSIRGSDVDAALHYLARLVEAGDLASIC
                     RRLMVIGYEDIGLGNPAAAARTVNAVLAAEKLGLPEARIPLADVVVDLCLSPKSNSAY
                     MALDAALADIREGKAGDVPDHLRDSHYKGAKSLNRGVGYQYPHHFDQAWVNQQYLPDK
                     LKNAQYYQPKDTGKYEQALGQQYYRIKEWKKHPPKN"
     gene            25781..26452
                     /locus_tag="IXT20_000634"
     CDS             25781..26452
                     /locus_tag="IXT20_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HAQ4859107.1"
                     /translation="MTDKQKIDEFFWNKTTPEVARDLLGMYLEHDTPEGRLAGYIVDA
                     EAYLGPEDEAAHSYGLRRTPRVRAMYEKPGTIYLYTMHTHRILNIITQPEGIPQGVMI
                     RAIEPAAMIDQMSKNRGGKTGPDISNGPGKLVEALAIPQELYGQSIADSSLRLVFEKK
                     KTPKKINALPRIGIPNKGVWTEKPLRFVVSGNPYISLQRKNQIEKDWGWRKENEKEGS
                     INIFR"
     gene            26418..26906
                     /locus_tag="IXT20_000635"
     CDS             26418..26906
                     /locus_tag="IXT20_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3013 family protein"
                     /protein_id="HAQ4859108.1"
                     /translation="MKKKDLLTYLDEIIEKQVTDYDVALDWDTKNHTIEIVVRLFAEN
                     KAQFEIDDAEGIVSQEPIIEFEDGVLLFNPQKSVFDERNYLAVIPYEGKKGLAKSVAD
                     SLVEYLNEVLAQGQSDLLDFLDEDDDEAVFELHWDNQQLAELVEVKQQNEADTYLPYP
                     SY"
     gene            26919..27866
                     /gene="prmA"
                     /locus_tag="IXT20_000636"
     CDS             26919..27866
                     /gene="prmA"
                     /locus_tag="IXT20_000636"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HAQ4859109.1"
                     /translation="MKWNEVKVETESEAVEAVANILMEAGASGVAIEDSLDVEHFQSD
                     QYGEILDKNDFTSLKEGALVMAYFPETTFLPEILPFIKDRITQLPEFGLAIGKNKVTV
                     SEVEESNWATAWKKYYHPVRITRMLTVAPSWENYQTEDPLEKIITLDPGMAFGTGTHP
                     TTSLTLTALETVLRGGETLLDVGTGSGVLSIAAKYLGAKDVYAFDLDEVAVRSAKENM
                     DMNEIAKDVHVSANDLLTGIEIESDVIVANILADIILLMIPDAWRLLKQTGTLIVSGI
                     IEEKKQMVLDAMLEQGFIVDQIFQQKDWYAIILKKPEVE"
     gene            27867..28631
                     /locus_tag="IXT20_000637"
     CDS             27867..28631
                     /locus_tag="IXT20_000637"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HAQ4859110.1"
                     /translation="MQRYFLKESYESKDYFVLEGENYHHIVRVMRMEPEDEVFLAFND
                     QIAIVAEITEITEEAVYLKEIKKEENQKELPSSITIASGYPKGEKLDWIVQKSTELGA
                     QAFLGFPASASVVKWDAKKRMKRQQRLEKIAQEAAEQSHRQIVPSVELLETKQDLFER
                     LDDYDVVLVAYEESAKQGEKSNAAKILSETKPDSRILAVFGPEGGLSRDEVDYFVEHK
                     GILCGLGPRILRTETAPLYLLSAASYQWELMNPSFS"
     gene            28864..31077
                     /locus_tag="IXT20_000638"
     CDS             28864..31077
                     /locus_tag="IXT20_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HAQ4859111.1"
                     /translation="MTKEEILTGPGVIKLVSQYMGPEHVAFVEKACEYATAAHDGQFR
                     KSGEPYIIHPIQVAGILADLKMDPHTVATGFLHDVVEDTEITLEDLREEFGDDVAMLV
                     DGVTKLGKIKYKSHEEQLAENHRKMLLAMAQDLRVIMVKLADRLHNMRTLKHLREDKQ
                     RRIAQETLEIYAPLAHRLGISRIKWELEDTALRYLNPKQYYRIVHLMQTKREEREKYV
                     SGTVEDIRIATEELGIFAEIYGRPKHIYSIYRKMKDQKKQFNEIYDLLAIRVIVDSIK
                     DCYAVLGAIHTKWKPMPGRFKDYIAMPKANMYQSLHTTVIGPAGNPVEIQIRTQEMHE
                     IAEFGVAAHWAYKEGKNEKVEPDGMTKQLSWFHEILELQDESYDASEFMEGVKGDIFS
                     DKVYVFTPKGDVTELPKGSGPLDFAYSIHTDIGNKTTGAKVNGKMVQLDYKLKNGDII
                     EIMTSPNSFGPSRDWLKLVATSKARNKIKRFFKAQDREENVIKGHESVVKCITDLGFT
                     PKDILTKNKLQEALDRFNYQTEDDLYAAVGYGEVSPLTMANRLTEKERKEQKIEQQKQ
                     EAEEIMNQPKKEPDKMKVRHEGGVVIQGVENLLIRISRCCNPIPGDDIVGYITKGRGI
                     SIHRRDCPNVQPDKPNVAERLIEVEWEDTSNTRKEYDADLEIYGYNRSGLLNDVLQTV
                     NALTKNLNSVEARTNKDKMATIHLTVGIQNLSHLKSIVDKIKAVPDVYSVRRTNG"
     gene            31088..31534
                     /locus_tag="IXT20_000639"
     CDS             31088..31534
                     /locus_tag="IXT20_000639"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HAQ4859112.1"
                     /translation="MRAVVQRVSKAAVSVDQQEIGKIGYGLLVLLGIHETDTQKDVDY
                     LVKKIAQMRIFEDEQGKMNISVEDVGGQILSISQFTLFADTKKGNRPSFVEAARPETA
                     IPLYESFNEGIRSRGITVATGQFGADMSVSLVNDGPVTIIIDSKNK"
     gene            31705..32964
                     /locus_tag="IXT20_000640"
     CDS             31705..32964
                     /locus_tag="IXT20_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HAQ4859113.1"
                     /translation="MKEVPVVDIKITDLKKLYQKGYNMLEELRHEADAPVVKAKIFNK
                     EAITIYGSSAAKVFYDPRNFKRKGAMPKLVLKTLFGQGGVQTLDGAAHHHRKNIFMDL
                     MTPERMEDYHRILDKNLTQALEAQHGQFELFDLSKMVFFTSICEWAGINLSAISKDEV
                     EKLAEYQISMISGTFTSPIDHIKGVENRKKSEKWAQGLIEEARQNPVAGKENVALYAF
                     ANATDLDGQLLPLEVAAVELLNIIRPTVALTVWAALMGHALFSRPDLYQQLKNDFSTL
                     QDPFIQEMRRYYPFFPMLPAISLKEVEVDGYRIPEGSWVILDLYGTDHDERTVEAPDS
                     FMIKRYVGKAKDISYKEEYEMIAQGGGNFRQMHRCAGEWITLHSLRVFSDQLVNKFEF
                     SVPEQDWTIPFNQFPTYPNSRALLYKN"
     gene            complement(33053..34000)
                     /locus_tag="IXT20_000641"
     CDS             complement(33053..34000)
                     /locus_tag="IXT20_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HAQ4859114.1"
                     /translation="MAEIRIGHSQVYAEQLGLGANAVGGHNLFDGLEDETGKQVVRTA
                     LNSGINLIDTAYAYGNGRSEELIGEVLKEKEYDRSRVVIATKAAHVPNKGRTFDNSPE
                     FLKQSVEDALKRLQTDYIDIFYIHFPDESTPKNESVATLHELKEAGKIRAVGVSNFTL
                     EQLKEANADGYVDVVEDKYSLIHRQAEKELFPYLEKNKISFVPYFPLASGLLTGKYEL
                     GEEKQFGEGDPRKRNPDFQGERFREILTAVDVLRPIAKRYQATPAQLVLAWYMKNPRV
                     SVVIPGAKRPEQVSDNVQALDLHLSNEDYQTIDEAFRGF"
     gene            34394..35686
                     /locus_tag="IXT20_000642"
     CDS             34394..35686
                     /locus_tag="IXT20_000642"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HAQ4859115.1"
                     /translation="MSFQRPKGTNDILPGESEKWQFVEETARLLFRDYQYNEMRTPIF
                     EHYEVISRSVGDTTDIVSKEMYDFYDKGERHVTLRPEGTAPIVRSFVEHKLFGPEYAK
                     PFKTFYMGPMFRYERPQKGRLRQFHQIGVEAFGSENPATDVESMVMALDFFKQLGITQ
                     IRLVINSLGDKETRQAYRQALIDYLLPFESELSEDSKRRLHENPLRVLDSKDKRDQKF
                     VAEAPSILDYLSEPAQKHFDTVVSMLDALAVPYEIDSNMVRGLDYYTHTIFEIMSDAP
                     KMGAQATICAGGRYDNLVEELGGPSTPGFGFAMGIERLLLTMEAEEVVIPAFNELDAY
                     VVALGDETNIEALKVVQAIRNFGFSADRDFMNRKAKAQFKTADKANAKLVLVIGEDEL
                     ASQTINVKAMASRKEKSFPLEEIYEHFDEVYDEMTLFE"
     gene            35701..37470
                     /gene="aspS"
                     /locus_tag="IXT20_000643"
     CDS             35701..37470
                     /gene="aspS"
                     /locus_tag="IXT20_000643"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016251448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HAQ4859116.1"
                     /translation="MAQRTIYCGLVSEQQLEQTVTLKGWVQKRRDLGGVIFIDLRDRE
                     GIVQIVFNPEINKEAWEIADKCRSEYVIEVTGEVRRRGENAVNPKMATGEFEVIASEI
                     VILNQAKTPPFQIEDDQAISDEIRLKYRYLDLRRPKMTKNIKLRNATTKAIRHYLDDN
                     DFLDIETPYLGKSTPEGARDYLVPSRVHAGHFYALPQSPQLFKQLLMNAGFDRYYQIV
                     RCFRDEDLRGDRQPEFTQVDIETTFLTAEEIQTYTEGLIAKVMKEVRGIEVTLPFPRM
                     TYDEAMARYGSDKPDTRFDMELIDLSDTVKEVEFKVFQMALENGGVVKALNAKGAADR
                     YSRKDMDQLGQYVGQFGAKGLAWLKVEEDGLKGPIAKFMGEATEAIIKATDAKPGDLL
                     MFGADKSEIVAAALGAIRTRLGKELGLIDESKFNFLWVTDWPQFEFSEEEGRYVSAHH
                     PFTMPKEEDIPLLAEDPAKVYAEAYDIVLNGYELGGGSIRIHTRELQEKVLETLGFTK
                     EQMEEQFGFLLTALDYGFPPHGGIALGLDRFAMLLAGEDNIREVIAFPKNGKAADVMS
                     DAPSMVSPLQLFELNIDVTNIEE"
     gene            37579..38454
                     /locus_tag="IXT20_000644"
     CDS             37579..38454
                     /locus_tag="IXT20_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAQ4859117.1"
                     /translation="MKKTFENWAYHDYTTKLSVSIVYALIASVALNFFYQPGHIYSSG
                     ITGLAQILTTLSKSLIGFEVPVSTTLYLLNIPLFVLAWLKIGKKFTIFTFITVTLTSL
                     CIHILPETVLSTDPIICALFGGAVMGLGIGLALRNGLSSGGLDIVSITIRKKTGKSIG
                     SISIYFNALIVFVAGILFGWQYMFYSTLSIFVSGKVTDVVYTKQKKMQVMIVTKHPEK
                     VVNEIQNCMRRGITIINEAEGAYKHDKQTVLMTIVTRYELPLLREAMKASDPQAFVSI
                     AENVQILGRFYEDGI"
     gene            38720..40603
                     /locus_tag="IXT20_000645"
     CDS             38720..40603
                     /locus_tag="IXT20_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HAQ4859118.1"
                     /translation="MEKQKRLKNSRYITGFDGLRSLAVVGVIFYHLLPTSMKGGYLGV
                     PIFFVVSGYLITDLLRQEWEQNGKINIWQFYVRRMKRLYPALVFMLVTASAYITLFQR
                     GLLNNLRGVVVSSLLYVNNWWQINNGLSYFDRFANESPFTHIWSLAVEGQNYLVWPIL
                     FVLLMVFVRKRKWIFYTVLGTSFLSAILMMILYTPGGDPTRVYYGTDTRLFSIWLGSA
                     LAFVWPSTRLKKNIPKQAKNILNIAGGISLLALILCFFFLDDHYNFIYYGGMLLVSLL
                     CVVLVAVTAHPGASLNRWLTNPVFTWIGKRSYGIYLYQFPVMIFYEAKVTDIADHVLL
                     HTLVEITLILIISELSYRYVERPLARFDYGRTFQVMKGWFSKPIISKSKPWVVPGTLL
                     VAIALVGFVTAPKNAVTADQKQLQAKIAESKKLAEETRNNSENTTSSVDQAILDKYEL
                     SKAQGNKAQKLELTAFGDSVMLDAATDLQELYPKVVVDGDVGRQLYASVPYIEKLKEQ
                     DLLKDTVLIGLGTNGSFSEAQFDSVMDAIGDRKVYWVNVRVPTKRWQNDVNAMLEQMT
                     KKYDNMTLIDWYSYSNDHSDWFYDDQVHPNPEGMTHYIHLVSEALLGGSSSTNSSETN
                     VTE"
     gene            complement(40678..40962)
                     /locus_tag="IXT20_000646"
     CDS             complement(40678..40962)
                     /locus_tag="IXT20_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HAQ4859119.1"
                     /translation="MANEHYFYVLFCKDQTFYGGYTTDLSRRLEEHNNGTGAKYTRLQ
                     KRRPVKMIHAEVFSSRSEATKAEAAFKKLTRKQKERYLQTHSTVLLPESI"
     gene            complement(40952..41686)
                     /locus_tag="IXT20_000647"
     CDS             complement(40952..41686)
                     /locus_tag="IXT20_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HAQ4859120.1"
                     /translation="MLHEDERIDQLYAEDIKIIQSSKVFSFSLDAVLLAHFSRVPKKG
                     KIVDLCAGNGAVGLFLSKRTQAAIDAIELQPRLADMARRSIALNHLNEQMTVHTIDLK
                     DSLSVVRHNSCDLVVCNPPYFKGLPTNKTNPNKHLAIARHEIHTTLEEVIDISSKLLK
                     TNGRFTMVHRPDRFLEIIQVMEDYRIAPKRIQFVYPKPGKEANILLVEGIKEGKLDGF
                     KVAPPLITYNMDGEYTAEIREMLYGK"
     gene            41815..42438
                     /locus_tag="IXT20_000648"
     CDS             41815..42438
                     /locus_tag="IXT20_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HAQ4859121.1"
                     /translation="MFYRFMRGVVRVLLALVNGNARYENKEVLPQDENYILVAPHRTW
                     WDPLYLAVAAAPKEFSFMAKEELFKNPVLRFILTRSNAFPVKRENPGPSVIKTPVKIL
                     KSTNLSLIMFPSGTRHATELKGGMALISRMSKAKIVPAVYQGPLTLGDLFKRQRVTVR
                     FGEPIDLSDIKKTDKEGMAEIERRTQAAFDKLDKEVNPDFKYEVKKK"
     gene            42630..43751
                     /locus_tag="IXT20_000649"
     CDS             42630..43751
                     /locus_tag="IXT20_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HAQ4859122.1"
                     /translation="MRFLHTADWHIGKKLQGYDLIEDQRHVLSSILDIAKEEKVEAIV
                     IAGDLYDRSVPAVDAVELYNELMVDWNLKEKIPIFAISGNHDSSTRLSAGTPWFSYSN
                     YFLRTQLAEAFEPVEFKNVQFFFLPYFEPIAARLYFEDDNIRTIKQAVAKVIDKMQEL
                     FDSEKKQVLISHFFVMGSVKSDSETKIEVGGLDSIPGELLTPFDYVALGHLHNQAALR
                     QDNARYSGSPLKFSLSEINQKKGVWIVDLTTGLELNFREIEPLHDIEEITASFKELLD
                     PAFYQSVNRENYIYIYLTDRTIIPNMMNQLRQIYPRILGIERANGRAEQTKRKRKVLE
                     MKDPQKLAEAFFSEMTEEQLTANQEKWLGETLQEIMEME"
     gene            43758..46886
                     /locus_tag="IXT20_000650"
     CDS             43758..46886
                     /locus_tag="IXT20_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC family ATPase"
                     /protein_id="HAQ4859123.1"
                     /translation="MKPKKLQLKNFGPFINETVDFSRLTEAPLFLISGKTGAGKTTIF
                     DGITFALFGETSGRLRSGKEMRSLFATPDEETSVTFSFEHQQMSYEIERKPEQVLAKR
                     KGNGTKKQAAKVTLTIFDGKGKEIKQITKRTEADQLIRELMNLDAKQFSQIVLLPQGE
                     FRNFLVSSSSDKETVLRHLFGTQFFQQFNERLKEKAKQQQQKLDHLEQELHLLQKRFV
                     LVKEEEPAAGFEAVLAQWTNHQAILEKQITEEKEALTELQEKQKQLENSYYRFESLKK
                     SYEEKTILINKQKELAAQKDEIEEKKRWIGYYEFTQRLVEPIQRLQEIKVEKIELSQK
                     SSEQTKKLAMTTQNYEEWQKNEANRVRRQTKIKEAAQKLQNKQMMVPIVESLRSKKQD
                     AEQLTISLDKEELNAEKLYQNLAIYQERTRILQENLLDQEKLQEDRLAFGQLKYLDEK
                     QQEIEKQKAKDTGSLAQIREELKKATEEITKNSQLLKEQTEQAEQLKSQWAKQQIARL
                     QLFLRPGEPCLVCGSTEHPIQQLKHEQPNKEDILKVEQQLAEAESAVERTQQIIAKSE
                     AHENNLQQQLSDLEQKIMATNIQLAEQEEHLQQALKKAFPEITEQKTKTTLVVIEQSL
                     DRRKSKMANARIELEEIRQQAEKLEQQLAVYKQQQTEKNNQLQQLQGEIKSLEQQLQD
                     TDSNCLEEEIRRLEKQLEEDSNQLAVEQKAGVQLLQDLHTLRTQLDLQHEQIEKIDEK
                     IEKEQERIAKKLESQSYFSTIEEICAFAQQQAQYKENQRTVTSYHEEQLVTADRLRQL
                     ENIQLTETFPDTEEISQQLEEIRRMIEIKQEKIYGLQEQKISNQKIYDECLSIYHTSQ
                     TKLDELTQLQQLSQTFNGENPKKTSLERYVLQVYLQEVLQVANDHLQRLTKNRYQFEL
                     ADTIGSYRGKTGLEINIYDDQAGMSRSAHTLSGGESFIAALSLALALADVIQTQAGGV
                     AIDALFIDEGFGSLDEEALEMAMEALETIESEGRMIGIISHVRELKERVLQQIRIDTE
                     GSGQSKIRYQLG"
     gene            46905..50099
                     /locus_tag="IXT20_000651"
     CDS             46905..50099
                     /locus_tag="IXT20_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HAQ4859124.1"
                     /translation="MKWSIPEKVIARGRQYLQEGRVLSVVPDQEENVWHAEVLGSELY
                     MVDLDGTAKEIDYCQCPYWEEHKYCKHTVAVELYLRSKNLSRIMKKDQPMLEPVKATS
                     EGEIIAKGFSRLKTQNNPQAVQPLIIEYQVETIETNQYHPELSILALYLRIGSLASPK
                     KTYIVKNIYDFLQAYTEKETYTVNKQYQFRLDKRAFQKEDQKILTHLAGSAQTHQLLG
                     TNGLQVKGKLDKRYLLLPVEQARFLLEQMNQTTRLHLQINEQNDQGIQFSDQNKPLSF
                     KVEKFGEDYLLQALNDFDFFFMHYQWGVIQGTFYSLTKYQQTVYQTWKQLIRRLEKPE
                     VVFKKKELPSLFKEIIPLLSEIGDVAIAPAVEEEVADIPVEFIFFFRKAKGKIQARVD
                     FVYGEVIYSTDPKHEVSTEQAFQVLRDTVEEKRVLDLFRMYRYQKNETGYERPLPSGE
                     ELYAFFRTELSVFRQFGEVRLGRKLRELYLDAHKHQPKIEVTETGSWLDIRFDVTGIE
                     EQEIDHVLQSLLRNDAFYTLENGQVLSFDSEEFQQTSQVLQQLRESIRTEEGTIHVPK
                     NQGLIIQNQLEKSNATFSESFQTMVQDLIHPERYQAQLPKGLNATMRDYQKQGFRWLK
                     MLGHYQFGGILADEMGLGKTLQTIAFLLSEKEERKSFSALIVAPASLIYNWQAEVRKF
                     APSLSIQVINGNKKEREELLAKDTDIRVTSYASLRQDLANYQSQKIDYLILDEAQMVK
                     NSSTKTAQALRELAVPQRFALSGTPIENNLEELWSLFATIMPGFFPTKTKFRELAAEE
                     IAQMIRPFILRRDKQTVLKDLPEKTEMNLYSALTEEQKTVYLAYLRQMREEITSMDSE
                     AFKKNRIGILAGLTRLRQICCDPRLFIEDYQGGSGKLEQVKDLLVAAKENKRRVLLFS
                     QFTGMLTILQEELAELGISTFYLRGSTKPQDRLSMVDAFNAGEKDVFLISLKAGGTGL
                     NLTGADTVILYDLWWNPAIEEQAAGRAHRIGQKNVVEVWRMISEGTIEERMDSLQQEK
                     RELFQKVIQGNEEQLTKLTEEDIRMILSVGEIEE"
     gene            complement(50245..50952)
                     /locus_tag="IXT20_000652"
     CDS             complement(50245..50952)
                     /locus_tag="IXT20_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HAQ4859125.1"
                     /translation="MIKSVVFDVDDTMYDQQQPFRNAVKRVVPLVSDADMHPLYIRFR
                     HHSDENFPKVMAGDWTLEYMRAHRISQSLKDLDYPHITEEDGLLFQKIYEEELDNICL
                     HEEVKKTLDFLKEKNVPLGIITNGPTDHQTKKLKQLQLNNWIPSRNMLISQATGFQKP
                     EKEIFQLAEKEFHMLPEETLYVGDNYDNDVLGAKSANWQALWFNHRERKIEQTPICDI
                     EISSFDQLLETMKTIFV"
     gene            51717..52115
                     /gene="spxA"
                     /locus_tag="IXT20_000653"
     CDS             51717..52115
                     /gene="spxA"
                     /locus_tag="IXT20_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Spx"
                     /protein_id="HAQ4859126.1"
                     /translation="MLTLYTSPSCTSCRKARAWLQEHQIPFVERNIFSEPLNSSELKA
                     ILQMTEDGTEEIISTRSKVFQKLNMDLDDLPLQELLELVQNNPGLLRRPIMIDDKRLQ
                     VGFNEDEIRRFLPRDVRQLELRQAQLMAGL"
     gene            52447..53100
                     /locus_tag="IXT20_000654"
     CDS             52447..53100
                     /locus_tag="IXT20_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HAQ4859127.1"
                     /translation="MEMEHINENTIRVLIGHEDLEERGVSFLDLLGNHKEIENFFYSI
                     LEEVDIEEEFRGSEAVTFQVLPKGDGLELFISKNLPDEEISQSEDSTVDSEDVSDFLR
                     QQIIGSDPEEELSGSALEEQERQATFAFEDFEQVIQLATDVELESAWTDLYLFEERYY
                     LVVHFWMENLNQADVENQIARILEFAEKSDRTPETLNEYGQCLMARNAIERTKFYFV"
     gene            53326..54324
                     /locus_tag="IXT20_000655"
     CDS             53326..54324
                     /locus_tag="IXT20_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein CoiA"
                     /protein_id="HAQ4859128.1"
                     /translation="MLLAMNEYNKCCFAKHAEKGKKYYCRGCRKRVILKKGKKKCAHF
                     AHQKSDNCSVFSERESKEHLQLKECFMDWLGQSVEPVFLEAYLPRLRQRPDILLANLA
                     IEIQCSRLSHQRFIERTQNYLNNSYQVWWILGNSFLGQSQFSLIEKSCCYYNRKRGVH
                     CWKADLKKQKLYLYHHITETVSGHISFCSSCWTFSSRDLKEIFTSNEIKMNQMKKIEK
                     VSEDGKKWLTRQLIHKQKNTVSIQEQCYLRHKHLLHLSSWIYQKSRFFFYLQEQVFLY
                     RMLYEEALENQKVPDFNSWLCQIKEHKREWLFPMIDEEMVYQQFFNECIHLSSLKK"
     gene            54500..56308
                     /gene="pepF"
                     /locus_tag="IXT20_000656"
     CDS             54500..56308
                     /gene="pepF"
                     /locus_tag="IXT20_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HAQ4859129.1"
                     /translation="MEVKQLPKREELPENLTWDLTKIFSSDQEFDEKYLELSEELKQS
                     EKHKGTLDQGASQFLNAIEFVLRVYRQTEVIYVYAHLKNDQDTGNTDYQALYARASSL
                     FSKVSEAVSWFEPEILQLSDDQIWQYFKEEPKLEVYRHYIQQIVDNRAHVLSAEQESL
                     LAGAGEIFDASSDTFAVLNNADLVFPTIEGENGEIVQLSHGVYGQLLESTDRRVREAA
                     FKGLYSVYEQFRNTFASTLGTHIKGHNFKAKVRNYSSAREASLSNNHIPESVYDTLVD
                     VVNKHLPLLHRYMELRKRLLEVEKLHMYDLYTPVLGEAPITFTYEEAKEKALEALKPM
                     GEEYMAIVEKAFSERWIDVVENKGKRSGAYSSGSYDTNPYILLNWHDTLDQLFTLVHE
                     MGHSVHSYFTRSNQPYVYGDYSIFLAEIASTTNENILTEYLLETEKDPRVRAYVLNHY
                     LDGFKGTVFRQTQFAEFEHFMHTEDEKGVPLTSEYLSDSYGKLNAKYYGPAVEEDPEI
                     KFEWSRIPHFYYNYYVFQYSTGFSAASALAKKILNQEPEALENYLAYLKAGNSDYPVE
                     VMKKAGVDMTQAAYIEDAMSMFEQRLNELEELIDRL"
BASE COUNT        18555 a         9727 c        11686 g        16421 t
ORIGIN      
        1 aaggcaaaaa gatgaactag aaatagttca tctttttttg tttaaatctt ttgctaagtt
       61 gaactaatag agtcttttat gaatatcgaa gttagttttt cacctaactt cgttttttat
      121 agttggttat tataaaatag taatatagct gatttggatt caattcatat ttatttataa
      181 atcaaaaata cagagaatag tatataaatg tcctttccta ttcttaaaat gatgtttgta
      241 cttacttaaa ttaggtataa ttagtatatg ttttgtgaga aatttagact tacaataagc
      301 agtgaattta taatcaacgt ataggagaca tggatgaaat gaaactaatt ggtatagatt
      361 tagacggtac tcttttgaat tctgaccaga gcattagtca aaaaaatgca agaacaatga
      421 aacatttacc ggaagactgt tttccattca tttgttctgg aagagaagta gaagacatta
      481 ataatattct caaaaaatct ggtttaagga taccagcagt agggttaaat ggttctgttg
      541 gatttgatgg agaaaaaaag atatttgagt cttccttcga taaaaatgat gtaaaaaaaa
      601 ttgattctgt cctatcgaaa tttccaacca aaatatatac aaatctcgga agttatgaat
      661 ctcagaatta taaaaataaa atgaaaaaaa tttttcacga ggtagggagt gaattttcta
      721 tcaaagagct aaactatgag cttgagtatg aaaaatctat ttgttcaatc ccttataata
      781 atattgagga aattatagaa aaggatgcta tcagcattta caaatttttt atttttattc
      841 ctaatcgtca attgaaagct aatatattta accatttgaa aaagatgcta aatgtgtctg
      901 ttactgaatc ggcacctgta aatattgaaa tcgtacctca aaatgtatct aaaggtgtgg
      961 tgtttgatcg tttggaatca atctacaatt taaagaaacc tttgagaatc gcgatcggag
     1021 atagtttaaa tgacttatct atgtttgaaa gtgcagatat tagttttgca atgggaaata
     1081 gtcatcaaga aatcatgaaa aaagctgatt atgtcactgt ttcaaatgat aaagacggag
     1141 tatctcaagc atttgaaaga attcttacca tataaactaa ttaaggtagt atagaacata
     1201 gaattctaaa tacggaaaaa tggagcataa attccagtac aaatagtact tcttcagtgt
     1261 caaacgaagc gaatataaca tagatattta ggattgtgac aaaaatcatg tcacaatctt
     1321 tttttcgaat aaacttattt tttgaagtcg gccatttatc gtaatttctt tgcataatac
     1381 gaacaaattt tcggttattg atgaaaaaaa acattgaaaa tggtatgata aaagaaaaaa
     1441 aggaggaaat ttttaatggt gaaatttatc catgcggcag atttacatat ggatcggtca
     1501 tttgaaggac tgacaactct tgataaaact gtacaggaaa aactattgaa gacgaatctg
     1561 accgttttat caaatatcat tgaacaggca ataataaata acgttgattt tgttttactt
     1621 gcaggagata attttcatca aaatcgtcct tcattaaaaa tacagaaaca tttttcagaa
     1681 cagatgaaac ggttagaaaa gaaccatata cctgtattta taatattcgg aaatcatgat
     1741 ttctatcaaa aagagcggta ttggttctcc tttcctaaaa atgtccattt atttacatcc
     1801 gaaacggtag agacaaaaaa aataacgata aaaagtggag aaactgtctc tctcagtgga
     1861 tttagttatc gtcaaccgtg gattcaaaaa gacaaagtga tggaatttcc ttctagagaa
     1921 ctcacagatt accatattgg gttgtaccac ggggaacctg gcgtctctca aaaaggaaac
     1981 tatgctcctt tccaaccctc aaagatgcaa gaaaagggat acgattattg ggctttaggg
     2041 catatccatg tgccaacggt cttaaatgaa aagcaaacaa ttgtttatcc aggtgcgcct
     2101 caaggtcata ctaaaaagga acaagcttct acatctattc tactagttga attaagtaaa
     2161 ggaagttgcc aaatacatcc gattaaagta gcagaggtct attggaagac aaaggaaata
     2221 tcattgagga aagcaagaac gacaaaagag gtcattgttc acattcgtca gcagctttca
     2281 aaagtagagg atggatttag tttaattgag ataaaattaa aagattatga ccatctcaat
     2341 acagatgttt tggaacggat acaatcagga gagctattag actatttgtt agaggaattc
     2401 tcagatagga taaatgattt ctttatttgg cgtatttctc tcatagagaa tactttttat
     2461 acgaagaccc cacttgctgc ttctgctaaa ttgatggaac agttatttca atactatcag
     2521 acacctcaag atttccaaca aattttaaat gaagtatata gtcatcaaga agcggctaga
     2581 atattgagcg aactgccgga atatcaagaa gaaacacttg aaaaagccaa gcagctgctt
     2641 aatcaagatt ttttatttga ggaggatcag gaatgaagat tttaaggatc gagattgctg
     2701 cttttggaaa atggcgtcaa aaaagttttg acttttattc aggcaatcag ctgatctatg
     2761 ggggaaacga agcaggaaaa tcaacgattt atcaatttat tcaagcaata ttatttggtt
     2821 tcccttcaaa aggccgtaaa aaaaaggatt atacaccaaa agacggctca gcctatggcg
     2881 gcaaaatatg gcttaaacat cctgtatatg gtgaatttgc tgtcgagcgc tacaagcagc
     2941 aaaatcgcgg aaaatcgaag gtgtggctgg gggatcaagt gggaagtgat gagttactag
     3001 aaaaactgat cgcacctttg acgaaagagt tattccagca ggtatttact ttccagcaag
     3061 agcagttgat ggaactagat catttgagag aaaaagagct acatgatgcg ctgatttctt
     3121 taggcatcac gggcagttct caagtattcc aaaagcggca agaatattat cagaaagcac
     3181 aacaattatt caagaaaaaa ggccaaaaat tactaatcaa tcaaaaatta aatgaatgga
     3241 aagagctgca agaaaaaatt catcaaaaac aagaacagga agcttatttt ggcaagttga
     3301 tccaacaaga acaggaatac gagtcgcaac agcagcagtt attggcacaa ttaaaagagg
     3361 cgaacaaaca attttcatta gccagacagc aagaacttaa tttttcttta tatgaagaat
     3421 ggcaatctct caaaaaaaac agcactacag gtcttcctga tgagcaactt cttttagatc
     3481 tggagtcttt ctccaaacgg tatcagcaaa caaatgagcg tttggaagaa ttagaagcaa
     3541 cactggaaaa gcatagcggg atggatcagc agtcagccag atattatttt tatttagagc
     3601 aggagcagga actaaaagat ctccagcaac ttcaatttac agcggaacaa ttaaacgaag
     3661 aatggcaaag attacaaatt gagactagag aaacaaattc agaattgaag attttggaac
     3721 aaaggtggca ttggcagaaa aatccgttac cggaagaatt tttatatgaa gaagaatgga
     3781 agcatcttct ggctgaagaa agaaggattg ctgatctaga aagccagatt tctgtgcgtc
     3841 tccaaatagc taaagaacag caagagatga ttgaagcaga agtgaatgac ttggaagaga
     3901 cctatccagc attattacaa aatcagtcag agatagcacc agtctcttca tctaaaccgt
     3961 atatgctgat ggctggcgga gcaataatca ttgcaggtat atttgtacca atgcccttga
     4021 aagtgatcct tttactaatt ggtgcaggca tgattggcta tttcttctac aaaaggcaac
     4081 caaaaattaa aactaaaaat gatttctctg agataaaagg aatgtggcaa gaaaaactag
     4141 gacaacttga tctagcctta gctcaattag ctgaagcaga agaaaaacaa aagagaactg
     4201 gagaagaaaa acagattctt cagcaagaag tatatcgttt tgcggatcat cggcacttag
     4261 gaaagatgga tacgctcaaa ctcatgaaag aatatggaca ggaagtcata gattatcaga
     4321 aattatatgg cgtatttgag cggaaacata aaagagaaca ggaaatacaa gaaaaactac
     4381 gacaaatcga ccaaaaattt gagtttgtta aagaatggtt accgatacaa gataagacaa
     4441 tttcagaaaa aatggtattg ctttcccgtt ttatacaaga gatggaacag atgaaatttg
     4501 ccagaagcta tcagcaaaat actttattga agcaagaaat caatcaattg aaaaagaaac
     4561 aaaaagtatg gatacaagaa tacagcgaac aattgactca agcaggctta tcttatcctt
     4621 cggaagtacc tgtcttttta caaaaagcca aacaagccga acagcagatg aaaagataca
     4681 atgaattatc ggaaatgctt actccactat ttcctgaaga gctgacaaaa gagaaattat
     4741 ctgtaagact gcgccaactg aaagaaaagc aatcccaact ccagcaagaa gaaaaagaag
     4801 caagtgaaaa tcggcaacga ctccaacttc aagtagaaga gttgcaaaga gacggcacat
     4861 tggacgaact ttatcaacaa gctagccggc aaaaagcaaa attacaggaa ttactggagg
     4921 aatggggagc acttgaaatc gccgggacat tcttaggtga tctttcaaca gagatgagtg
     4981 aaagacaatt accccagttg ttaagagcag caggtgatta tttaggtatt ttaacaaaag
     5041 gaaattatca gaagatatta ctgaaaaatg atacactgac tttaacagac ggatatacag
     5101 aatttcccat ctatgagtta tctacaggta cgaaagatca gctgattatg gcgatgagat
     5161 ttgcttacct ctcattagaa gctaaccgtt cgatatgccc gatcatcatt gatgatggct
     5221 ggcttcatta tgatcatcag cgaaagtatc aattagctga acttttagct aaatttggag
     5281 aaaagtatca aataatttgt ttttcttctg ataaagaaat ggtaagctat taccatgaga
     5341 tgaaccaaat cgtaaaacaa ttagaaggag caaataatga aaaaaattcg tgatttagta
     5401 atcgatgagc agtttgaaca atttgtcctg atcaaaagtg cagatgtaag aatcgctaaa
     5461 aacggaaaaa aatttattgc ttttactttc caagatactt caggtactat cgatggtaaa
     5521 tactgggatg cttcggagga ggaaatcaat aaatttacag ccggcacagt cgtttttcta
     5581 aacggtaaaa gagaggttta tcaaggaaac cctcaagtaa agattcttca tatgcgctta
     5641 acaagggtag atgagccagg agagcctagt ttatacatgg aaagagcccc tttgaagaaa
     5701 gaagaaatgg aagaagaaat caatcaattg ttgtttgaga tcaccaatgc gcattggaat
     5761 cgtattgttc gctatctact taataaatat caaaaacaat tttttgagtt tccggcagca
     5821 aaaagaaacc atcatgcatt tgccagtgga ttagcttatc atactttgac aatgcttcac
     5881 ttagggaaat caatcgccga tgaatatcct gagctgaatc gagcattgtt gtatgctggt
     5941 atcattttgc atgatttagg aaaggtgatt gaactttccg gtcctatgtc gacagaatat
     6001 acgcttgcag gaaatttgat cggtcatctg gtattgattg acgaagaaat aactaaagcc
     6061 tgtcttgcgt tgaaaatcag cgaagctgac gaagatgttc tcgtattacg tcatatggtt
     6121 ttagcacacc atggcttatt agaatatggc tcacctgtac gtccaagaat aatggaagca
     6181 gaaatattac atcaaatcga caatatcgat gcttcgatgc agatgatgtt gacttcgatt
     6241 cgtcaaacag aaccaggaaa atacaccgat cggattttcg gtatggacaa ccgaagtttc
     6301 tatgtaccaa aagatttata gcaaaaattt atttttttca ttcaaaacaa aaaaagcgag
     6361 acataagtct tcgctttttt tgttttcttt tattttgatg aatcagttgt ttcttcagtt
     6421 gaagattctt ttgttgaatc agttgatttt gtgtcagaac tctttgttga agctgtagtt
     6481 tcacttgagt ctttcgtact tgatgaagtt gttgtgaatg cactcaacac attttcaaag
     6541 gcatcatctt tgatttttac atttgcatct tttaattctt caccgattac tttagttgtg
     6601 aatgcattgt cacttagttt agtttcagta gcgatatctt ttagttgatc tttgtattta
     6661 tccatgtcat tgcctttatt ttggtttttc accattttga caacgtagta ttctgtcgca
     6721 taagaagtag gatttgttgt agtaataaca tcggagatct caccgtcttt caatttgaaa
     6781 gctgcttctt ttacttctgc aggaatagtt gttgtcgttg agtcgaattt aactttaccg
     6841 ccatcttctt ttgtctctgt atcagttgat ttgtctttgg ctaatttaga gaaatcatct
     6901 ccatcatccg ctgatttttt cacatctttt gcttcatctt cacttgataa tttgatgatt
     6961 tgtgcttcta cttctggatg gaaagatttc caagcagttt tcaaatcgtc atctgtaata
     7021 tctacgtgag ctttcaagcc agcttcaaaa gctaaattgt tacgaatata ttctttataa
     7081 gtatctttcg tatatccagc agcttctaat tgtgattcaa aagtatcacc taatgatttc
     7141 gcttgtttgt cgtattctgc atccacttgc ttatcggtta ctttatcgcc atatttgtta
     7201 ttgaatactt tataaatgat catccgttgt actaaggatt ggttcgatga ttcaagtttt
     7261 gcttcatcat agaaatcaga tacagtgatc gttccgcctt tcatcgttgc gatatcttta
     7321 ttagtatcgc ctgaacaagc tgctagagtt aaaacagcca acgcactagt agctgccaaa
     7381 ataattgatt tttttttcat taaaatttgc actcctgtaa ttagttatta tttttatata
     7441 ttaactatac catatcccat ttcctttaaa aacgttgtcg tggtagtttc aaacaaaatt
     7501 caaaatttca tcacattcct aggaaatacg caaacttttc tcacttatat agttaaatct
     7561 gttgagaaaa taatcattat tatatttgtt caggatactc tggcagatca gcttttagtt
     7621 tttctacttg ttcattgatt tgtgtgatac gtggttcggc ttggaattta aaatcttcga
     7681 tatctttttt gattgcttct tggaaagcag gaatcacaga ttcagctgtt tcttttgttt
     7741 cgatcaatgc gcttttaaaa tgattcaggc tgttatttaa atcatttgtc agagttgttg
     7801 cttcttccaa ttcttcgatc agtttctttc ttgtttcatt cccgctcctg ggcgccaata
     7861 acagacctcc gacagtgcca gcagcagctc caaataacag tcctttgaaa aagtttttaa
     7921 tcatcgcagg acacctgctt tacgattgtt tcttgaatag cttttagttc ttccgatcca
     7981 taactatctt gatgattgac aaagtgaata gaaaaatcat cctctttgct gtagcgaggg
     8041 actaaatgga tatgtgaatg aaaaacagat tgataggcta gttcacggtt gttattcaag
     8101 atattaagac ctttcatttc tggaaaagct ttttccaatg cttgagcaat cttaggcaca
     8161 cgcgaaaaaa cctctcctgc aagttcggga tcatattcaa aaatatctgc tacatgacgt
     8221 ttaggcacaa ctaatgtgtg tcctttcgtt gcttgagaga tgtctaaaaa agcatagacc
     8281 ttgtcgtctt catatatttt atagcttggg atttcttgat tgatgatttt gcaaaaaata
     8341 cagttttcca tataaaacat cctttctata aagaataaat acttcagctt aactttacca
     8401 taagttaaag ctattgtaag gaaaaaaaca aaataccgtc attttcttcg tggtataata
     8461 aataaaaata aatggcggag gaaaaagaat gagtttaatt atcgatcatg tcacaggggg
     8521 gtacggacat ctccctgtgt taaaagatat cagtttcgat gtacagcctg gagagatagt
     8581 agggttgatt ggactgaacg gagcagggaa aagtacgacc atcaagcaca ttatcggtct
     8641 gctggaagct tcaaaaggaa cgatagaaat caatcatcta tccttaaaga aaaatacgga
     8701 agaatatcgc aaacaaattg ggtatattcc agagactcct tctttatatg aagaattgac
     8761 attgagagaa catatcgaag tcactgcgat ggcctacgat atacctatgg atatagcaat
     8821 gaaaagagca gaaaagttgt tggagacttt tcggttatct aatcgtcttg actggttccc
     8881 agctaatttt tcaaagggaa tgaaacagaa agtgatgatc ttatgcgcct ttttgatcga
     8941 gccttcttta tatatcatcg acgaaccatt tttaggcttg gatccactag ctatcaatgc
     9001 attgcttgaa ttgatggtag aaatgaaaaa agaaggagcc ggtattttga tgtctacgca
     9061 tattttagct actgcagaaa aatattgtga tcgttttgtg gtcttacata acggagagat
     9121 acgagcagac ggttctcttg agatgttgag aaaagaattt cagctgccgg attcttcttt
     9181 ggacgaaatc tatattgctt taacaaagga gcaggcacat gagtagtttt tttcaaaaac
     9241 gtttagcaag acatcaaaag aaaatgatga aatatatgcg ttatgtattg aacgatcatt
     9301 ttgttttagt ttgtcttttc ttgattggcg gtgtgggatt atattattct aattggttga
     9361 aaaccctgca gccaccgttt gcaattggag ggctcataat ctctgtattt tggatgctct
     9421 gtttattcgt gggtaagatg gctacttttg cagaggcagc agatattgtt tttttacttc
     9481 ctaaagaaaa agaaatgcaa acctatttgg aacgagcttt tcgctattct tgcatttttc
     9541 cgttgatctt tttgatattg gcatgcggat tggccatgcc attagtggtg gttagtacgg
     9601 agcaatcctt ttcgatgttt ttcgtctatc tgatcatatt atggagtttg aaattcagtc
     9661 atctgcaaat caagagagca gaattgtttc gactagatca acaagttatt cgttattgga
     9721 aattaggctg gtttttttct tctctgatct ttttgtttct atcattttat tggctaatat
     9781 gggcaggaat agcaggtgca ttagtacagg caagtctata ttactggttt ttgtggaagc
     9841 aacaagaaaa aggattggat tgggagaaaa tggtgcatgt agaagaacat aggttgcatc
     9901 agatctatca atttatcaat ttattcacag atgttcctga aattacggca aagatcaaaa
     9961 gaagacgcta tttggatcct atattgactc atattaaaag agaatcaaaa aacacttatc
    10021 tttacttgtt tgcaagaaga ttggcaagag gttctgattt tagtggtctt tatttccgat
    10081 tagtaattat aggtgggtta ttgattgccg gagtagtcga cttttatttt agtctgatta
    10141 tcggtttctt atttttatat ttgatcggtt tccaactgtt acctttagcg aaccagttcc
    10201 gctatatggt tctcgttcag ctttatccaa tccataagaa tcagaagaag caggcgattc
    10261 agtatctact cacatgggta ttaattatcg cgtcggttat ttttggtttg attgctgcag
    10321 tcgtattaga tgggcagcag cgatgggttc cgctagcggc ttatttgatt gttactatgc
    10381 tctttgttaa actatatgta ccaactcgtt tgaagaagat gtcagactga aaacaaagct
    10441 gtgtagggta aaaatgagaa tgaaaaatag agcgttgaca gtaagaatgc tgttcgttct
    10501 attttttttg agaaaagctg gttttttgaa tggcaattaa atgaaaaaga acgtatatgt
    10561 gcttgacgtg tagtgttcag gcgactagaa taataagtac tgcaaaacaa agagtaagag
    10621 gacgggagaa tgcatgatgg agtttcagtt agaccaagag tggcggttac gtcccatcaa
    10681 aggcgacaca ggaaaaacat acataggcat gcgtgatgag gacaaagtct ttattaaacg
    10741 aaacacgact ccgatgttgg ctgctctttc aagggaagga attacaccaa aacttgtttg
    10801 gacaaaacga acggggaatg gcgatacttt gacagcacaa gagtggctgg aaggtcgctt
    10861 gctgactcct aatgagattg gtcaaagaaa cgacgtgatt gatgttttgt accatttaca
    10921 tcattcaaat tctcttaaaa ccatgctgac ccgtatcggt ggtcgtgtca tgacgccgga
    10981 gaagatgctg caaaaatatg aaaaagaatt accggaagaa ctccagaaaa atcaatttct
    11041 gcaaattgtc catcgttttc ttcgtgagca gatcccgtca atagataagg aaaacttatc
    11101 agtagtacac ggagatgtaa accatcgtaa ttggttagta tgtaaaaatt atttatatct
    11161 agtcgattgg gattcaatca tgtttgcaga ccctgcggtg gatattgcaa cgattttagg
    11221 aaattatgtg ccactgtcta gctggaatca gtggctgatc agttatggca tacgcccgac
    11281 tgacgaaatg cttgaaaaat ttcattggta tgcggccatg aatttgctcc aagaaatcac
    11341 cagatattgc aaacgtgaag atcatcgacg gatgaacgaa gaaattttac aattaaaacg
    11401 aattttcagt ggctgaggtt tggcccaaac tctgcggaga tttggccaga cctttttaat
    11461 tagaggaaag gaatggagat aaataatgcg tgtccgaaat cgtccgggag cagcggaaat
    11521 gttagcggct cacccgaatt ttgtaattag tgatccaaca ttgtggaagg gaaaatggaa
    11581 tgaattgttt gaaaacgatc atccaatcca cattgagatt ggtatgggga aaggccagtt
    11641 catcactgga atggcaaaag cccatcctga aatcaattat attggagtag aaatgcaggt
    11701 cagtgttgtt tcgattgcac tagacaagtt gatcgaacaa ccgcttccta acctgaaact
    11761 tctacatgtg gatggatcag cgttgacaga gtatttcgct gacagcgaag tagatcaaat
    11821 ctatctgaat ttttccgatc catggccaaa aaaacgtcat gaaaaacggc gattgacata
    11881 taagactttt cttgctgtag atgaacagat tcttcgtcca aatggagaaa tccatttcaa
    11941 aacagataat caaggattgt ttgagtattc tctggcaagt ttttctcaat atggcatgat
    12001 cctcaaacag gtatggttag atttacatca aagtcaattt gaaggcaata ttatgacgga
    12061 gtatgaagaa aaattttctt caaaaggtca acgtatttat cgagtagaag ctcaatttca
    12121 agataagacc aaaaagaaaa gtctgtgaaa ctaactttct gctttcataa aaacattaat
    12181 atccataaat aaatcccaga aaatgaacat tacagatgta atgtattttc tgggatttat
    12241 ttgatttaaa atgtatttat ttcatcggtt tttccacaag gtggaatcaa ttggaagcga
    12301 gaaaatgaaa ataatccttt gtcggtgatc ttcaattcgg gtatgacaga taatgttaag
    12361 aaagaaagtg tcaaaaaagg atcaaaggtt ttagctgctc ctagttgatg tgctttagtt
    12421 attaattcat tcaactgttc atagacgatc gaaaaaggtt cctgtgtcat gattccgcct
    12481 ataggaagag aaacacaagc tatttcctgc tgtttgttga caacggtcat cccgccacct
    12541 ttttgaatca aaagattagc agcatacagc atatcctcgt cgtttgtccc aacgacaact
    12601 agattatgcg agtcatgggc aatcgttgtc gctatcgctc cttgtttcag ctgaaatcct
    12661 ttcactatgc ctaatccaat gtttcccgtt tgatggtgac gctcgatcac cgcaatcttt
    12721 aattggtctt tttcgattga aggaatgaat cgttcgcctt gtcggtccac tttttctacc
    12781 aaatctttag tcactagact attcgggata atttcgatca catgggcttg tgaagaagaa
    12841 agaagcagct ctaacgaatg tggatttaat ggttgagcgt ttagttttgg cagacaacac
    12901 gtatatgaat ttgaatgatc agcaaataaa tggtcttgga gttgcccaga ttgaacaact
    12961 aatgttccct tatagaaaac ttcatgaatt ggcagcttgg taagatcgtc tgttaagaag
    13021 aaatcagctt gatacccagg ggcaatcgca cctagtgttt tcaaatcaaa acattctgct
    13081 gcattcaatg ttgccatctg aatggcttgt aatggatcga ttccaagtgc aatagcttga
    13141 cggacgatat gatcgattga tccttctttt actaaatcat tgatcagttt atcatcggtg
    13201 acaaagaagc atcttcttga attttcaggt gtgatcgccg gtagtagcga tgccagatct
    13261 tttgcaacgg tcccttctcg aaccattaaa tacataccaa gatccaaacg ttctttagct
    13321 tcttttgcag ttgtagcttc atgatccgtc cgaatacctg ctgcaggata aacatttaac
    13381 tcttccatgc cgataccagc tgcatgcccg tcaatttttt tcttatgctg gtgcatcaat
    13441 tggatctttt ctaggatgtc tgtttcatta tttgctactg cttgatagtt catgacttca
    13501 gctaagccta aaacttcggg tttttgtaaa aaaggggcta atttttttgc atttaaattt
    13561 gcaccattgg tctcaaaagg ggttacaggt acacaagaag gtagcataaa aaagatattc
    13621 atgagtgttt gtcttgcatc ttctatcata aactcaagac ctgtactccc agcaatgttt
    13681 cctatttcat ggggatctgt cactacagta gtaactccat gaagcaagga tacttttgaa
    13741 aattcgcttg gtgataaaag tgagctttcg atatgcacat ggccatcgat gaatcctggc
    13801 actagatatt gcccattagc attatgtacg ctttttcctt cgtattcacc gattcctacg
    13861 atcattcctt cgcagattgc tacatctttt tccatgattt ctttcgtaaa gacgttgacg
    13921 atcttagcat gcttgatcac taggtcagca ggcttttttt tacttgctac ttcgatatat
    13981 ttccttaatg ttgtttccaa tggagtgcct cccaagataa atttttataa gtataacata
    14041 aattcggttt ttgccaatat ttttttgtaa attcatttta tttgaaacaa aaagatcaaa
    14101 aagacgtatg ttttttgtgg aggggagaat aatggaaata aaacgattat cggatcaaat
    14161 gataagtgaa agttttgcca aacatgccca agacgtatat atattgccag cttcagaagt
    14221 attttacatc tattttcgct atggaaaaaa acgaacattc tggaagaaag tctcttcggc
    14281 agatggacag caactgatct caagatttaa gtatttggga cagatggata tcggagaaaa
    14341 aagaaaagct cagttaggtg catttactta ttctttagaa aaacaggaag ttcgtctgcg
    14401 gttgtcttct gtaggagatt accttcaacg agagagttta gtgattcggc tattatacgg
    14461 tacttttgaa acaccccatc gttgctttag ttctcgagat tttgcaacta ttttacagaa
    14521 aaccgtcaga cgtggattat accttttcag cggaccagtc ggatcaggca agacttctct
    14581 aatgtatcgg ttggctttgg aagaaaaatt gcaagtgatt actatcgaag atcctgtaga
    14641 gatagaagaa ccgttttttc tgcagctaca gatcaatccg aaaatagcac agacttatga
    14701 tacacttttg agactcgcat tacggcatcg tccagacttg ctaatcattg gcgagataag
    14761 agatgaatca actgcacatg ctgcggtaag agcagcacta acagggcacc gggtttttgc
    14821 aaccgtacat gcacgcaatc ttgaaggtac attggtcaga atccatgaac tgacaaagaa
    14881 aaaagaagag ctatctgaat gtttagctgg tgtcatttat caagaacttt tggaagatgc
    14941 caatcaccat ccttctgttt tatggagtta tcaatttatg gaggaagaat cagtaaaaaa
    15001 atcatgggag gaaagttatg aagaagctca ggaaaatatt tggcagtcag aactattcta
    15061 aaggattaac aagaagccag caaaaccagt ttgcacaaac catgggagaa ctgcttttat
    15121 ctggttttag tctgcaagaa tccctttatt tattactcac gatccgttcg tttccacctg
    15181 ttattttggc aaatatccaa catcagctaa aggaagggca agcgtttagt gaggtacttt
    15241 cgcaaatagg atatggagca gagcaactgg tacagatcga gttagctgaa cgtcatggga
    15301 atctgccgac aactttgttg gaaattgcca aacagtttcg gcttgttgaa ggatttcgca
    15361 atgatttgaa aaagctgctt gcatatccga tgctattgct cacctttttg ctaggtatct
    15421 tgtttagtat gcggttgttc gttttgcctc agcttattgg ttcaggaatg gtcatgcaag
    15481 agcattgggg tattcagctg atacaagtct ttcattggta tgttctacta ttcatcctag
    15541 cggttactct attggcttta ctagtaaggt atcgtctgat gaagttgtct cctgttgatc
    15601 agagtgaatg gataagcaga caattattga ttggaccgat atattccttg tatcaatcgg
    15661 cttatttgtc actagagatt ggaaagcttt ttcatgaagg tttcgaatta aggcaaatca
    15721 tagtctgctt aaaggaaacg aaacaagaaa gtctgattca gcatcttgcc tctaagttga
    15781 ttcaaggatt ggaggcaggt atgtcattgg ctgagcagtt ccagaattat cgttttctat
    15841 cagaagaatt cagtaaaatt gttttacagg gagaggcaaa gggaagttta ggtaaagaac
    15901 tgatttttta cagcgaatgg acaagaaaac agcttttcaa acgtcttcat cactggatgc
    15961 attttattca gccaatcatt tttttgggcg tagcggcttt gattttatta gtttatgccg
    16021 ctgttttatt acctgtttac ggaagcatag aggaggtttt gccatgaaaa agagaaaaat
    16081 atcttattat agcggtttta cattgatcga aatgctaatc gtcttactga tcatcagtgt
    16141 attagtgttg ttgttcgtcc ctaatttgtc gcgttatagg aatcatgtag accaagaaag
    16201 tcgtgaagcg atcatccagt tggtagacac acaaaaagag ctctatgcgc ttcaaaataa
    16261 tggaagagtt cctactgtgg aggaattgtt gaatgaaggc tatatcaaac gagaacatgc
    16321 agaaatctat cagcggcctt gaaggtaagg ttttagcatc aggatatacg ttgtttgaat
    16381 cactcttact gattttatta cttacaacga tcctttcttt tccagttctc gcattttctg
    16441 catggaagaa tgaattggca gtctatcaat ttttcggcca atttgaaaaa cgaatctatt
    16501 ctacgcaaaa aatcgctatt gtgaatcaga tccaaacagg cttttattgg aatagtgaag
    16561 agaatcaaat catttttcat gttccgaatc cagataaaat aaattgggaa gtattagata
    16621 ttcctgatga gattaccttg aaacgccatg cttctattac ttttgctgct agaaccggga
    16681 atgaaagttc actaaaagct tatcaatttt attgggaaga aaaagagcgg acgattacgt
    16741 atcaattcca gatagggagt ggtcgctata caaaaagaat tgaataaaac aggatttgtt
    16801 ttgttggagt cccttacagc gcttatgatc gcttctgtct gtgtctactt agtgagcttt
    16861 ggccagctgt ttttgcttca ggcagaaaag aaaagtgggg aggaactgct atttattcga
    16921 atgatttatg aagatatcaa gacgcagcgg atgtatcaca aaagaccaga gcgaagggaa
    16981 gaaatgatcc catacgatat ccaaattgtc gataataatg gagtaatcga aaaagtgttt
    17041 gtcaatatgg agaaagaagc gatcagtatt gaaaaaacaa gtaattccag catttacgct
    17101 gattgaatgt ctaattggac tgatgatatt gagcagtcta ttgctcacat tcaatctttt
    17161 gattcagcaa accatccaag taagtgaatt tttgcagcgt aaagatcaaa aagagtggct
    17221 aatattttta gcacaattag aagaagaact caagagcagt cacaatgtct ctgtgaaaaa
    17281 tcagcaactg cactacacga tcgaggataa acagtatatc atcgaaagat atcaggcaat
    17341 gatacgtaaa agaagaactt ccggcggaca tcaacctatg ttaacaagta tcactgagct
    17401 tcaattattg gaaaaagaac acacaatttc tttttatgta cactttgaaa atggagaaga
    17461 tggatatgcc atatggacaa aaaacgatca gtagaaaatg ttataagggc tctattttat
    17521 tttctgtctt gcttttattt cttttatgta cgtttttatt gatggcagca ttagaaagct
    17581 accgtttgtc aatggatctt aatatacgaa tcaaaaatta ttatatagca aaaataatga
    17641 agcagatggc tttatcggaa cagtctacgc ttgccgaaag ttcggaagga gttttttact
    17701 acacaactgg atctgtaaca tatcagtggg tacaacagtc cctattctta gaggtggaag
    17761 tctcaccgtt tatctttcgg ttcacagaag aagtaaaaaa tgatacagat actagtacag
    17821 aatgatggaa tgttcgtgaa aacgccctct tttctgttat aacactgttt ttaataaaaa
    17881 aatgtttgcc gtttcacaaa taaaaaacag tgaaaacgat tgaaattttg tcaattgttc
    17941 gatagaatta aacaggtgaa aaaattgtta taatatatct agaaatgagg tgcaccatgt
    18001 taccagaaaa aatggaacaa gggtttaatc aaaacctgga agctattcag cttttacaga
    18061 atgcattggg aacttccttt ttagaagcct atgtcgaaaa tgcagaaaat ttaatagatg
    18121 attatcaagt acgtgtggtg gatggagtgc cgacaaaaga gacaacgcaa cggatcacag
    18181 ctctttatga agagttgaag aaactttctt tcgagccaga agaatggcgc agactgtctc
    18241 aattactttt attgaagggc agccagacag aacatttgca ggcgaaccat caactcactc
    18301 ctgatagtat cggtttttta tttgtttttc ttattgaaca actatacaca aataaaaaag
    18361 caccggttaa gattctggat attgcggcag gaatgggcaa cttgttatta acagttcttt
    18421 taaacctatc gaatgcgggg taccaaacag aaggaattgg tgtggatatt gatgatacat
    18481 tgttggctgt cgcagcttct acaagtgatt tgacacaagc aaatgttcaa tactttcatc
    18541 aagacgggtt gcaggaatta ttaatagatc ctgtcgattt tgcaatcagc gatttgccaa
    18601 tcggttatta tccgaatgac gaaaaagcaa aagagttttt aacaagtacc gaagaaggcc
    18661 atagttatgc acatcattta ttgctagagc aatcaatgaa atatgtgaag ccggatggtt
    18721 ttggtttgtt tttgatgccg agtggatttt tagaaacgga tcaaagtgaa gaaatcaaaa
    18781 aatggtttaa agaagaaggt tatcttcaag gaatgattca attgccagat gaattgttcc
    18841 gtaataaaca atcgcaaaaa agtattttga tcttacagaa aaaaggacca caagcacaac
    18901 aagtaaaaga agtcttgctt gtgaaacttg cttcattaaa agaaccagag aaggtgacgg
    18961 aatttttcaa cgagtttaaa aactggaaat catctgctct gtaatcattc aacgagaagg
    19021 agagtcatat gtctaaaaca attgcaatca atgctgggag ttcaagtttg aaatggcaat
    19081 tatatcaaat gccagaagaa accgtagttg cgaaagggat cgtcgaacgt atcggtttac
    19141 aagattctat ttttaccatc aagtacggcg ataatcaaaa attcgaacaa atcatggata
    19201 tcgaaaacca cgaagtagca gtaaaaatgc tgttagatca gttgattgaa ctgcaaattt
    19261 taagctctta tgatgaaatc accggagtag ggcaccgtgt tgttcacgga ggagaaactt
    19321 atggggattc agtagtgatc gatgagaaag taatggaccg cattgctgaa ttagcagaat
    19381 ttgctccttt gcataatcct gcaaacttaa tggggatcaa agcgtttaaa aagattttac
    19441 ctgacatttt gagtgtggct gtattcgata catctttcca ctcaacaatg ccaaagcaaa
    19501 attatttata tagcttaccg gtgtcttatt atgaagattt caaagtaaga aaatatggct
    19561 tccatggcac aagtcatcgt tatgtgtcgg aacgtgcggc aaaattattg aacaaaccga
    19621 tcgaagaact aaaaatcatc acttgccact taggaaatgg tgtatcgatc acagcagtcg
    19681 atggaggtaa atccatggat acttctatgg gattcacacc attagctggg gtgacaatgg
    19741 gtacacgttc aggtgatatc gatccagctg ttctcccata tttaatggaa aaattagaga
    19801 ttgatattga tgaaatgatc aatgttttga acaagaaatc tggtctgcta ggcctaacag
    19861 gattgtctag tgatatgcgt gatcttgaaa agaactatga aaaagaacat attcgtttag
    19921 cttatgacat tttcgtggac cgcatccgaa aatacatcgg cggttatgtg acaacgatga
    19981 atggtgttga tgcaattgtc tttactgctg gtatcggcga gaatgacgga catgtacgta
    20041 gtgaaatcat caaaggtatg tcttggtttg gttgtgaaat cgatccagcg ttgaatgaat
    20101 tgcgtggtga agaagtagat atctctacaa aagaatcaaa agtaagagta ttagtgatcc
    20161 caacagatga ggaactaatg attgcccgtg atgtggagcg tctgcgcggc taagccaaag
    20221 cattcgttag gataaaagat gaaacgcatg caagagggaa ttcttgcatg tgtttttttt
    20281 gcggagaaga ccgcaaaatg gtaatttaac ttcaatttcc cttctttttt acaggatctt
    20341 cttagggaat tttgcttgga aaagtggtca aaagggaaaa gactggcatt ttatttattg
    20401 attttcagga aaatcccaat attttatttt cattcatctt tataagggac attacatatg
    20461 taaaaagaaa aaacattttt agatcagcct cgtgggctgt cgactttatt cttcgcttta
    20521 tgtttagcta gaaaaccgat ccgaaacatt cattgatcga ataataaaaa cttgattttc
    20581 cacctgtact ttgcctgaat cgtgaaagca caggtatgaa aatcaagttt tttgttgcga
    20641 aaaaacgtcc tagtcaatca gttggccgag cggctggcgg ctgatctgct cctgatcaat
    20701 aacgagtgct tcttggaagt gttgctgcaa aaagtagcgg tcatggctga ctgctaggag
    20761 cgttccttga tagtcttgca gcatttcttc aatttcttct cgagcatagc tgtcgaggtg
    20821 attggttggt tcatccaaaa tcaggacgtt gattttttct aaaaagagtt tcagcaacgc
    20881 caaacggatt ttttcaccac cggataaatc tttgatccgt ttacccacat cgctttgata
    20941 aaagccactt ttggctaatt gccgtcgagc gacttcttcg tttccagtca atgtgatggc
    21001 gtaagccagc aaacggctat cgggatcggc aaactgcagc tgctgcggca aataaccgat
    21061 ttgcaagcta ctacctaatt tgatggtgcc accgtaaggc ttttcctcac cgagtaataa
    21121 tttgagtaag gttgattttc ctgcaccatt gtcgccgatg atggctaaat gatctccacg
    21181 atagactgca aaactgctgt ctttgaatag tacttgatca gcgtactgct ggtgcaagtt
    21241 ttgaatgatg aaaacttctt tgccagattt tccaccatta gacaagcttt gcagccgatt
    21301 tttcggggga ggtggcggtt tgacgagtgt cagtttcgcc aggcggcgtt ccagttcttt
    21361 ggcttttttg aagaaggact cattgtctcc ttcatgtgcc cactgacgaa aacgacgaat
    21421 catgactttc aaccgctgga cttctttttg ctgcagttcg taattttttg tcagctcagc
    21481 caagcgagct tctttcaaga ctcgaaaacg gctgtagttt ccgggatact caatcaatgc
    21541 accttcttcc agttcccatg tttttgtggt gacttgatcg agaaattgcc gatcatgaga
    21601 aacgaccaga accgattttt ttgtattctg taaatagttt tccaaccagc gaatactgtt
    21661 aagatctaaa tgattggtcg gttcatcgag taaaagtaaa tcggcatcgg ctgctaaaat
    21721 tttggctaaa gcgactttaa ctcgttcgcc tccgctaagt tctgttaacc gtgcttcctg
    21781 acggtgtgga atccctaagc cttgcaagat cgctgcaatc cggtcttcta atacgtaacc
    21841 gcctagttct tcaaagcgat gctgcagctc accgtaacgg gccagacact tctctaagtt
    21901 tgtatctggt tctgtcatca atgcttcgta gcgactcatt tgctgtttga ggtcctgtat
    21961 ctcagcggca gaatggagca agtagtggtt gacggtcgct tcggaaactg taagttcttg
    22021 ttcgaccgtc ctgatcttta agcctttttt gcgactgata acaccgctgt cgacaccttc
    22081 tctgccggtc aagatttgta aaaaggttga tttgccagtt ccgttgatac cgactaaggc
    22141 aattttttca ccagcttcaa tttgacagga taaatcctca aataatggat tcccgccgat
    22201 tgttttttta atagaaattg cttgtatgat catgatctct ctccttgaga ccactcaaaa
    22261 aggccatggt tatccatggc ctgtgttatt taaactcaga ttatcatgag aaaaatacct
    22321 tgagatggcc attgatttta cgtgaattca actgttttgg acaaacttgt cccgtcaaca
    22381 gcaactgtaa aaccaattgc atggctaaat gcgatatggt atcgttggta attttcatta
    22441 cttgttattt tagtcatgcg catcatagta ttttccctcc ttcgtgtagt tgtcttttcc
    22501 agtgtacctg aaagtgtcag aaagttcaac tagcgctgac atttacttaa tatttatcga
    22561 tgtgtaattc tgtcagagag ctttttttat ctgactgagg gtaatgtctc gtggaccaca
    22621 tcattgtatt ctgagctaaa ccagcgttca ggcgaaatca tatggtgttt gataaaaaac
    22681 tttgtctaca tgacttcctt ttgccatttg cttaataccg ttgcattaaa cagctcttct
    22741 ctttggaaat ctcaccaaca tcgcgttcag gagtatcgtt gtggttgcaa tggcaagcgg
    22801 tagcctttca tcaattcttg aatgatttac cgaatgtagc acttgccttc tcgcggtacc
    22861 cgaacccgaa ataaaatttt agtttggcga taaaatcggt atccgccatc gatacttcat
    22921 attttttcgt atcagattca tcggaaactt ccacattgac gaaccaataa gctttggcat
    22981 gcttaggtcg ttttaaacca caccagggcg actaatcgcc ctggtgttca ctttttttta
    23041 ttttacttga actcattgac tgttttccct ttattttaaa cattataaag accgatgagt
    23101 gcttgccgcg tcttttgttt ttatcacggt tttttgtgca aaaaaacctg ccgatagttg
    23161 gcaggtcgtc tacgtgttaa gctttggtgt ggtctttagc tggcaattgg ttttctaaat
    23221 cagttcggac aacaagcaca tcgcaagctg cgtttctaat aacatattca gaaacagaac
    23281 caataaataa acgttctaca gcattcaatc ctgtggctcc taacataatc aaatctactt
    23341 gattatcttc tgggatttct ctggcaataa tttgttttgg tgagccatat tctacgacag
    23401 aggttacatt attcaagcca gctttcttcg cattgctttc ataatctgcc aatgtttgct
    23461 ttgccatttc agtagcttgt tctgcaagca ttccatcaaa ggatgaaacg gtttggaaag
    23521 aacgtgtgtc aatgacatgt gctaatagaa gttctccgtt atttcggata gctacattga
    23581 ccgccttttt aaaagctaat tcagcttcat cagaaccatc tacggcaacc atgatttttt
    23641 tgtattgttg taacataagc attcactcct caagtcaatc gtattgtact cttttattgt
    23701 agaacacttt agggaaaaag agaattattt actgctttgg ttcagcaatt ttttttgcaa
    23761 atcggatact gaaggtggca gaaacaataa ttacgatcag taaaaagacc aaggcaataa
    23821 agcgttgatt gaaaaaagct gttaaaatac ccattacgcc taaaacagca tatgcagctc
    23881 cgtaaaattg taggaatcct tgatttttat catttttgtt gatcaataca aaaaagatat
    23941 cctgtttatt aaataaataa tagctaacga ataataatac taaagtggcg ataatcatta
    24001 aaatcctaat catgaacaaa tcctccaaaa taagcaacaa atagaaaaag caatttgcca
    24061 agagcaaatt gctttattct ttaaaagatg gtaatctccg ttaatgccgt tgtttgtcct
    24121 gtagtattga tcccgcgaat tcaagcaggt gggtcccatg gtcgtagctt cgaactacca
    24181 aatgataagg catgttacca gcagtgacac aagacgggtc ccaagatatc aataaaaatg
    24241 ttcggtcaac acacaaagag gacaattcga acatcacaga tttcccacct ctatagtagc
    24301 atatgaattt aaaaagagca atgagagagc tcgcagaaac agaaccgttt tcttgtctag
    24361 ttttttggag gatgcttttt ccactctttt atacggtaat actgttgacc cagagcctgt
    24421 tcatatttcc ctgtatcttt tggctgataa tactgagcat tcttcagttt atctggtaaa
    24481 tattgctgat tgacccaagc ctgatcaaaa tgatgaggat actgataacc cacgccacgg
    24541 ttgagtgatt tcgcaccttt gtaatggcta tctctcaaat gatcaggaac atctccagct
    24601 ttgccctctc gaatatcagc aagtgctgca tctaatgcca tgtaggcaga attggatttt
    24661 ggcgacaagc aaagatcgac gacgacgtct gctagtggga tacgtgcctc aggcaatccc
    24721 aatttttcag cagctaatac agcgttcact gtacgagccg cagcagcagg attacctaaa
    24781 cctatatctt cataaccgat aaccatcaga cgccggcaaa tacttgccag atcacctgcc
    24841 tcaactaaac gggcgagata atgaagtgca gcatccacat cacttccacg aatcgatttt
    24901 tgaaaagccg aaataacgtc ataatgagca tctccatttt tgtcatgggt aagagctttt
    24961 ctttggacac attcttcaat gatcgacagt gtaagatgaa tccttccttc tttatctgaa
    25021 ggagtcgata gtgtagccag ttccagccca ttcaatgcac tccgcaaatc gccgttcgtt
    25081 gcacgcgaaa ggtgaagaag agcatcttcg tctaactgga ttgcttgttg tccaaggccg
    25141 cgttctttat cccttaaagc atgttcgact gccaactgga tgtcttgttc tgttaatggt
    25201 ttgacttcaa agatctgtgt acggctgcgg atcgctggat tgatggtaat ataaggattc
    25261 tcagtagtcg ctccgatcaa gatgattcgg ccactttcta aatgaggaag cagaaaatcc
    25321 tgttttgttt tatctaatcg gtgtacttca tccaataaga ggatcactgt accactcatt
    25381 tttgcttctt ccgctacaac ttgtagatcc ttttttgaat ctgtagcggc gtttagcatg
    25441 cgaaatgcat aattagtaga gccggcgatc gcacttgcga tactcgtttt tccagtacca
    25501 ggaggtccat atagaatcat ggaagagagc atgcgagcat cgaccatgcg gcgtatgatt
    25561 tttccttccc cgactagatg ctgctgacca acgacttcat cgatcgttcg aggtctcatg
    25621 cgataagcca atggctgttg cataggattt tcctccttct tgtttttacg tcccttaatt
    25681 atatcatatt tagcaagatg cctagaaaga acagccttct gttgttttgt tttcatagct
    25741 tatccaacta atattgtgta tgataaagta ggtgagaaaa atgacggata aacaaaagat
    25801 agatgaattt ttttggaata aaacgacgcc agaagtggca cgggatttat tggggatgta
    25861 tttggagcat gacacaccag aaggaagatt agctggatat attgtagacg cggaagcata
    25921 cctaggtcca gaagacgagg ccgcccatag ctatggttta agaagaacac cgcgtgtgcg
    25981 ggcaatgtat gaaaaaccag gtacgatcta cctttacacg atgcatactc atcggatttt
    26041 gaacatcatt actcagccag aagggattcc tcaaggagta atgatacgtg caatcgaacc
    26101 cgccgcaatg attgatcaaa tgagtaaaaa tcgtggcgga aaaactggtc cagatatcag
    26161 caacggtccg ggaaaattag tggaagcatt agcaattcct caagaacttt atggtcagtc
    26221 tatagcagat agttcgttac gcttagtttt tgagaaaaaa aagacaccaa aaaagatcaa
    26281 tgctttgcct agaatcggta tcccaaacaa aggtgtatgg acagaaaagc cgttgcgttt
    26341 tgttgtgtcg ggaaatcctt atatctcttt acaaagaaaa aaccaaatcg agaaagattg
    26401 gggttggagg aaagaaaatg aaaaagaagg atctattaac atatttagat gaaataattg
    26461 aaaaacaagt gacagactat gatgtagctt tagattggga tacaaaaaat catacgatcg
    26521 agatcgttgt tcgcttgttt gcagaaaata aagcacaatt tgagattgat gatgcagaag
    26581 ggatcgtttc gcaagaacca attatcgaat ttgaagacgg cgtacttctt tttaatcctc
    26641 agaaatctgt ttttgatgaa cgcaattatc tagcagtaat cccttatgaa ggaaaaaaag
    26701 ggttggcaaa atcagtagct gattctttag tggaatatct aaatgaagta ttagctcaag
    26761 ggcaaagcga tttgcttgat tttttggatg aagacgatga cgaagctgta ttcgagcttc
    26821 actgggataa tcagcaacta gcagaattag tagaagtaaa acagcaaaat gaagcagata
    26881 cttatttacc gtacccaagt tattaaggag tgacagatat gaaatggaat gaagtaaaag
    26941 tagaaactga aagtgaagca gtcgaagcag ttgctaatat tttgatggag gcaggggcga
    27001 gtggtgtcgc gattgaagat tctttagatg ttgaacattt tcagtcggat caatatggtg
    27061 agattttaga taaaaatgat tttacatcgc tgaaagaagg cgcactagtc atggcttatt
    27121 ttccggaaac gactttctta ccggaaatct tgccttttat caaagatcgt atcacacaat
    27181 tacctgagtt tggtctagct attggaaaaa ataaagtaac agttagtgaa gtagaggaaa
    27241 gcaactgggc aacagcctgg aagaaatact atcatccggt gcgtattacg cgaatgctga
    27301 cggttgcgcc aagctgggaa aactaccaga cagaagatcc tctagaaaaa attatcacac
    27361 tagatcctgg tatggcattt ggcacaggca ctcatcccac gacttccttg acattgacag
    27421 cactagaaac agtcttgcgt ggtggtgaaa cattgctaga tgtgggaact gggtcgggcg
    27481 tgctaagtat tgcagcaaaa tatcttggtg caaaagatgt ctatgccttt gatttagacg
    27541 aagtagccgt tcgttcggcg aaagaaaata tggatatgaa tgaaatagcc aaagatgtcc
    27601 atgtatcagc taacgatctt ttgacaggaa tcgagattga aagtgatgta atcgttgcga
    27661 acattttagc agatatcatc ttattgatga tcccagatgc ttggcgttta ctgaagcaaa
    27721 caggcacgct gatcgtttca ggaatcattg aagagaagaa gcaaatggtt ctggatgcca
    27781 tgttagaaca agggtttatc gtggatcaaa ttttccagca aaaagactgg tatgcaatta
    27841 ttttgaaaaa accagaggtg gaatagatgc agcggtattt tttaaaagaa tcatatgaat
    27901 caaaagacta ttttgtttta gaaggggaaa actatcacca cattgtacgg gttatgagga
    27961 tggaacccga ggatgaagtt tttttggcat tcaatgatca aatagccatt gttgcagaaa
    28021 tcacggagat tactgaagag gctgtttatc tgaaagaaat taaaaaagaa gaaaatcaaa
    28081 aggaactgcc aagttcgatt accatagcga gtggctatcc taaaggggaa aagctggatt
    28141 ggatcgttca gaaatccact gaattaggag cgcaagcttt tcttggtttc ccggcatcag
    28201 cctctgtcgt taaatgggat gcaaaaaaac gtatgaaacg acaacaacgg cttgaaaaga
    28261 ttgctcaaga ggcagcggaa caatcccatc ggcagatcgt gccatcagta gagttattgg
    28321 aaacgaaaca agatttattt gagcggctag atgattatga cgtggtcttg gttgcttatg
    28381 aggaatcagc aaagcaaggc gaaaaatcaa atgcagccaa aattcttagt gaaacaaaac
    28441 cagatagtcg aatcttagct gtctttggac cagaaggtgg attatcccgc gatgaagtcg
    28501 attattttgt agaacacaaa gggatacttt gtggattagg accaagaatc cttcgtacag
    28561 aaacagcacc tctttattta ttaagtgctg ctagctatca atgggaatta atgaatcctt
    28621 ctttcagcta acgttcttca ttaataaaag agtatcgaca gaaagaattg aaaaaatagt
    28681 caacaatttg tataatagaa acaattattt atacaatgga tacgaaaaat cgtttgaaaa
    28741 agcttttaaa aagcgaacat ttccgtaaaa aaacgtataa ctgcacggat tttgggacaa
    28801 ttatcaaaca gatatttgtc ccgattcccg ttttaccgaa aaaagaaagg gggatcgatt
    28861 cctgtgacaa aagaagaaat tttaacggga cccggtgtca taaagctcgt tagtcaatac
    28921 atgggtcccg aacatgtagc atttgttgag aaagcatgcg aatacgcgac tgctgcacat
    28981 gacggacagt ttagaaaatc aggcgaaccc tacattattc atcctatcca agtcgcaggt
    29041 atattagcag atttaaaaat ggatccccat acagtggcta caggcttctt acatgatgtt
    29101 gttgaagata cagaaatcac tttagaagat ctgagagaag aatttggcga tgacgttgct
    29161 atgttagtgg acggcgtaac caaattaggg aaaataaaat ataaatccca cgaagagcag
    29221 ctagcagaaa accaccgaaa gatgctgcta gcaatggctc aagatttacg agttatcatg
    29281 gtcaaattag ctgatagact acataacatg cgtacgttga agcacctgcg agaagataag
    29341 caaagaagga tcgctcagga aactttagaa atctatgcgc ctcttgcaca tcgtctaggg
    29401 atcagccgga tcaaatggga attagaagat acagcacttc gttatctaaa tccaaaacag
    29461 tattaccgta tcgtccactt gatgcagacg aagagagaag aacgtgaaaa atacgtaagt
    29521 ggtactgttg aagatattcg aatagcgaca gaagagctgg ggatttttgc agaaatctat
    29581 ggacggccaa aacacattta ttcaatctat cgtaaaatga aagatcagaa aaagcagttc
    29641 aacgaaattt atgacttgct agctattcga gtgatcgtag attcaatcaa ggattgttat
    29701 gctgtattag gagcaatcca cacaaaatgg aaaccaatgc ccggcagatt taaggattat
    29761 attgccatgc caaaagctaa tatgtaccaa tctttgcata ctaccgtgat cggtccggca
    29821 ggaaatccgg tagaaattca aatccgaaca caggaaatgc atgaaatcgc tgaattcggg
    29881 gttgctgcac actgggccta taaagaagga aaaaatgaaa aagtagaacc agatggtatg
    29941 acgaaacaat taagctggtt ccatgagata ctcgaacttc aagacgaaag ctatgatgct
    30001 tctgaattta tggaaggcgt aaaaggagat atctttagtg ataaagtcta cgtcttcaca
    30061 ccaaaaggag acgttactga gttaccaaaa ggatccggac cattggactt tgcatacagt
    30121 atccatacag atatcggtaa caaaaccact ggtgcaaaag taaatggcaa aatggtgcag
    30181 cttgattaca aattgaaaaa cggagatatc attgagatca tgacttctcc aaattcattt
    30241 ggcccaagtc gcgactggtt gaaattagtt gctactagca aagcaagaaa taagatcaaa
    30301 cgtttcttca aagcccaaga tcgagaagaa aatgtgatca aaggccacga atccgtggtc
    30361 aaatgtatta cagatctagg atttacgcct aaagatattt tgacgaagaa caaactgcaa
    30421 gaagcactcg atcgttttaa ttatcaaaca gaagatgatc tctatgcagc tgtagggtat
    30481 ggagaagtta gccccttgac gatggccaat cgtctgactg aaaaagaacg taaagaacaa
    30541 aaaatcgagc agcaaaagca agaagcagaa gaaatcatga atcagccgaa aaaagaacct
    30601 gacaaaatga aagtacgtca tgaaggtggc gttgtcattc aaggggtaga aaacttacta
    30661 attcgtatca gtcgctgctg taatccgatt cctggtgatg atatcgttgg ttatatcact
    30721 aaaggcagag ggatatccat tcatcgtcga gattgtccga atgttcagcc tgacaaacca
    30781 aatgtagcag aacgtttgat tgaagtcgaa tgggaagata catcgaatac acgaaaagag
    30841 tatgatgcag atttggaaat ttacggctat aatcgttcag gcttattgaa tgatgtactt
    30901 caaacagtca atgcgctaac gaaaaatctc aacagcgttg aagcacggac gaataaagat
    30961 aaaatggcga cgatccattt gacggttggt atccagaatt tatcccacct aaagagtatc
    31021 gtggataaaa tcaaagcagt acctgatgtc tacagtgtac gccggacgaa tggataggag
    31081 gacagatatg agagcagttg ttcaacgggt aagtaaagct gctgtgtcag tcgatcagca
    31141 agaaattgga aaaattggct acggcctact tgttttatta ggcattcatg aaaccgatac
    31201 gcaaaaagat gtggactatc tggtaaaaaa aattgcacaa atgcgtattt ttgaagatga
    31261 acaaggaaaa atgaatatta gtgtagaaga tgtgggagga caaattttaa gtatctctca
    31321 attcacactg tttgcggata cgaaaaaagg aaatcgacca agctttgtgg aagccgcacg
    31381 accagaaaca gctattcctt tatacgaatc attcaatgaa ggaatcagaa gtcgcggcat
    31441 cacagtcgct accggacaat tcggagcaga tatgtcggtt tctctcgtca atgatggacc
    31501 tgtaacgatc attattgatt ctaaaaacaa ataaagtaat ctggttttca ctagtaaaaa
    31561 ctatgaaaca cgtcgcttaa gttagactga taaactctaa cttaagtggc ttttttgtct
    31621 gcaaaaagcg cttcttttgt cagcagtcgg aaaaaaagtt aagcttaaat tatacaagtt
    31681 attttttata ggaggttgct gaagatgaaa gaagtaccag tagtagatat caaaatcaca
    31741 gatttaaaaa aattgtatca aaaaggatac aacatgttgg aggagttacg ccatgaagcg
    31801 gacgctcccg tcgtcaaagc aaagattttc aataaagaag ctatcacgat ctacggatca
    31861 tcagcagcaa aagtatttta tgatccaagg aatttcaaac gaaaaggtgc tatgccaaaa
    31921 ttagtattga agacactttt tggtcaaggt ggagtacaga ctttggatgg agctgctcat
    31981 caccatcgga aaaatatttt tatggatcta atgacaccgg aaagaatgga agattatcat
    32041 cgtatcttag ataagaatct gacacaagca cttgaagcac agcatggtca atttgaacta
    32101 tttgatttaa gtaagatggt tttctttaca tctatttgcg agtgggcagg tatcaattta
    32161 tcagcgattt ctaaagatga ggtcgaaaaa ttagcagaat atcaaatctc tatgatcagc
    32221 ggaacgttta cttctccgat tgatcacata aaaggtgtcg aaaatcgcaa aaaatcagaa
    32281 aaatgggcgc aaggcttgat tgaagaagct cgacaaaatc ctgtggctgg aaaagaaaat
    32341 gtggcattat atgcttttgc caatgccaca gatttagatg gtcagctttt acctctggaa
    32401 gtggcagctg ttgaattatt gaacatcatc cgtccaacgg ttgctctaac tgtgtgggcg
    32461 gcattgatgg gacatgcact tttcagtaga ccagatttgt atcagcaatt aaaaaatgac
    32521 ttttcgacgt tacaagatcc gtttattcaa gaaatgagac gctattatcc tttcttccca
    32581 atgctacctg cgatttcgct gaaagaagta gaagtagatg gttatcgtat accagaaggc
    32641 agttgggtta ttttagattt atatggaaca gaccatgatg aacgaacagt tgaagcgcca
    32701 gattccttta tgatcaaacg gtatgtaggg aaagcaaaag atatttccta taaagaagaa
    32761 tatgagatga ttgcacaagg cgggggaaat tttcgacaaa tgcatcgctg tgcaggagaa
    32821 tggatcactc ttcatagtct acgtgtgttt tccgatcagc tagtaaataa gtttgagttc
    32881 tctgtacctg aacaagattg gacgattcca ttcaatcagt ttccgactta tccgaatagc
    32941 cgagcgctac tttacaagaa ttagattagc ataaacttat cgaaaaaaag gaagatcaaa
    33001 cagtgctgat cttccttttt tcatattact ataaattgat aaggtcaatt gatcaaaatc
    33061 cacgaaatgc ttcatcaatg gtttgataat cttcattgga taaatgcaaa tctaaagctt
    33121 ggacattgtc agatacttgc tctggacgct tagctccagg tataactaca gacactcgtg
    33181 gattcttcat gtaccaagct aaaacaagtt gcgcaggagt ggcttggtat cttttagcaa
    33241 tggggcgaag tacatcgaca gcggttaaaa tttctctgaa acgttctcct tggaaatctg
    33301 gatttctttt tcgaggatct ccttcaccaa attgtttttc ctcaccaagt tcatattttc
    33361 ctgtcagcaa accggaagca agaggaaaat agggaacaaa actgattttg tttttttcaa
    33421 gataagggaa taattctttt tcagcctgcc gatggatcaa actgtattta tcttctacta
    33481 catcgacata tccatctgca ttcgcttctt tcaattgttc taacgtgaag tttgacacac
    33541 cgactgctcg gatttttcct gcttctttta attcgtgcaa tgtagcaact gattcatttt
    33601 ttggtgtgct ttcatccgga aagtggatat aaaaaatgtc gatgtaatca gtttgcagac
    33661 gttttagtgc atcttccacc gattgtttga gaaactcagg agaattatca aatgttcgcc
    33721 ctttatttgg tacatgagca gcttttgtag cgatgacgac acgtgagcga tcatattctt
    33781 tttccttcag tacctcgcca atcagttctt cggaacggcc gtttccgtaa gcataagcgg
    33841 tatcgatcaa attgatcccg ctattcaaag ctgtacgaac tacttgtttt cctgtctcat
    33901 cttccaaacc gtcaaaaaga ttatgtcctc caacggcatt tgctcctaat ccaagctgtt
    33961 ctgcgtagac ttgtgaatgt ccgattctta tttctgccat agctgattcc tcctttttgt
    34021 ctagtctaaa acaaaatacc aaaaagctga actaaaacgt ttttgtgagg ggaaatagtc
    34081 agatatctct tgactttgag actggttttt agtaggttat aagtaatatc gaatgtcaat
    34141 gaaagagcga gtaagttttt gaaactttac aagagaggat catcgaggct gaaaatgatc
    34201 taaagtttca ttagcctaaa gacactctgg agatggacga gtgaaaagcc gtcccgcgta
    34261 cgagcgttat tcgtaagagg aaagagaaat ctttcaaaat taggtggtac cgtgtatgaa
    34321 tttcattcac ccttgttcat gatgaacaag ggtgattttt ttatttacat agctaagaaa
    34381 gggagagaaa actatgagtt tccaaagacc aaaaggaaca aatgatattt tgccaggcga
    34441 atcagaaaaa tggcagtttg tcgaagaaac agcacgttta ttattcagag attatcagta
    34501 taatgaaatg cgtacaccga tttttgaaca ctatgaagtg atatcccgaa gtgtagggga
    34561 tacaacggat atcgtttcaa aagagatgta tgatttttat gacaaagggg aacgccatgt
    34621 gacgcttcga ccagaaggta cagcaccaat cgttcgttct tttgttgagc ataaattgtt
    34681 cggcccagag tatgcgaaac cgttcaaaac tttttatatg ggaccaatgt ttcgctacga
    34741 acgccctcaa aaaggacggt tacgtcaatt ccatcaaatc ggagtagaag catttggatc
    34801 agaaaaccct gcaactgatg tggagagcat ggtgatggca cttgatttct tcaaacagtt
    34861 ggggatcacg caaatccggc tagtcatcaa ttcattaggt gataaagaaa cgcgtcaagc
    34921 ctatcgtcaa gcattgatcg actatctttt accttttgaa tcagagctga gtgaagactc
    34981 taaacgccgc cttcatgaaa atccattacg cgtattggac agtaaagaca aacgagatca
    35041 aaaatttgtg gcagaagctc catctatctt agattatcta agcgagcctg ctcaaaaaca
    35101 ttttgatacc gttgtttcca tgctggatgc attggctgtt ccttatgaaa tcgacagtaa
    35161 tatggttcgc ggactagatt attacacaca tacgattttt gaaatcatga gtgacgcacc
    35221 aaaaatgggg gcacaagcaa cgatttgcgc aggcggacgt tatgacaatc tagtggaaga
    35281 actaggtggt ccttcaacac ctggcttcgg atttgcaatg ggtatcgagc gattactctt
    35341 gactatggag gcagaggaag tagttatccc tgcatttaat gagttggatg cttacgtggt
    35401 cgcactcgga gacgaaacaa atatcgaagc attgaaagtg gtccaagcga ttcgaaactt
    35461 tggtttttca gctgaccgcg acttcatgaa ccgtaaagca aaagcgcaat ttaagacagc
    35521 agataaagcg aatgccaaat tagtattggt gatcggtgaa gatgagcttg ctagtcaaac
    35581 gattaatgtc aaagcaatgg caagccgaaa agaaaaaagt tttccattag aagagatata
    35641 tgaacatttt gatgaagtct atgatgaaat gactttattc gagtaggagg aaaaaaataa
    35701 atggcacaaa gaacgatcta ttgcggatta gtttccgaac aacaattaga acaaacagtt
    35761 acattaaaag gatgggtaca aaaaagacga gaccttgggg gagtaatctt tatcgaccta
    35821 cgtgatcgtg aaggaatcgt tcaaatcgtc tttaatcctg aaatcaacaa agaagcatgg
    35881 gaaatcgcgg ataaatgtcg cagtgaatac gtgatcgaag tgactgggga agttagaaga
    35941 cgcggagaaa atgcagtaaa tcctaaaatg gccactggtg aattcgaagt gattgcaagt
    36001 gaaatcgtga ttttgaatca agcaaaaacc ccgccatttc aaattgaaga tgatcaagca
    36061 atcagtgacg aaatacgttt gaaatatcgt tatttggatc tgcgtcgtcc aaaaatgact
    36121 aaaaacatca aattaagaaa tgcgacaaca aaagcgattc gtcattattt agatgataat
    36181 gactttctcg atatcgaaac accttaccta gggaaatcta caccagaagg cgcaagagac
    36241 tacctagttc cttcacgtgt ccatgctgga catttctatg cgttgccgca atctcctcaa
    36301 ttgtttaaac aattgctgat gaatgctgga ttcgaccgtt attatcaaat cgttcgttgt
    36361 ttccgcgatg aagatttacg tggagaccgt cagcctgaat ttacgcaagt agatattgaa
    36421 acaacgttct taacagcaga agaaatccaa acttacacag agggattgat tgcgaaggta
    36481 atgaaagaag ttcgcggtat cgaagtcaca ttgccatttc ctcgtatgac ctatgatgaa
    36541 gcaatggctc gctacggcag cgacaaaccg gatacacgtt ttgatatgga attgatcgat
    36601 ctaagcgata cagtcaaaga agtagaattc aaagtcttcc aaatggctct tgaaaacggc
    36661 ggtgtcgtga aagcattgaa tgctaaaggc gctgcagatc gctactcacg aaaagacatg
    36721 gatcagttag gtcaatatgt aggacaattc ggagctaagg ggcttgcatg gctgaaagta
    36781 gaagaagacg gattgaaagg tccaattgct aaattcatgg gagaagcaac agaagcaatc
    36841 attaaagcaa cagatgcaaa accaggtgac ttgctgatgt ttggcgcaga taaaagtgaa
    36901 atcgtcgctg ctgcattagg tgctatccgg acacgtttag gaaaagaatt aggactaatt
    36961 gatgaatcga aattcaattt cttatgggta actgattggc cacaatttga attttcagaa
    37021 gaagaaggtc gatatgtctc tgctcaccat ccattcacga tgccaaaaga agaagatatc
    37081 ccgttattgg ctgaagaccc tgcaaaagtc tatgcagaag cttatgatat cgtcttgaac
    37141 ggttatgaac taggcggagg ttctatccgt attcatactc gtgagcttca agaaaaagta
    37201 ctagaaactc taggctttac aaaagaacaa atggaagaac aatttggttt cttgttaaca
    37261 gctttagatt acggattccc tccacatggc ggtattgcat taggattgga ccgttttgca
    37321 atgttattag ccggagaaga taatattcgt gaagtcattg cctttcctaa aaacggaaaa
    37381 gcagcagatg tgatgagtga tgcaccaagt atggtttcgc ctttacaatt gttcgaattg
    37441 aatattgatg taacgaatat agaagaatag ccgataaaaa ggtaaaaagg atttgtcgaa
    37501 tacgcttctt ttgacaacct tgatttaatt gaaaaacact ttataatgaa gccataacat
    37561 ataaagcggg gcttttccat gaaaaaaaca ttcgaaaatt gggcttatca tgattacacg
    37621 accaagcttt cggtatctat cgtttatgcg ttgatcgcat cagtcgcatt gaatttcttt
    37681 tatcagccag gacatattta ttcaagtggt attactggtc tagctcagat tttgacgact
    37741 ttgtcaaaat cactgatagg attcgaagta ccggtatcaa caactttgta tttactgaat
    37801 atacctttgt ttgtattggc gtggttaaaa atcgggaaga agtttacaat tttcactttt
    37861 atcacagtca ctttgacctc cctttgtatc catattttac cggaaacagt cctatctacc
    37921 gatccaatca tctgtgcgtt gtttgggggg gctgtgatgg gactaggtat tgggctagcg
    37981 ttgagaaacg gcctatcatc gggcggtcta gatatcgtga gtatcacgat tcgtaaaaaa
    38041 acaggaaagt cgattggttc tatttcgatc tacttcaatg cattgatcgt ttttgttgcc
    38101 ggaattctat ttggctggca atatatgttt tacagtacgt tgtctatttt cgtcagtgga
    38161 aaagtaactg atgtcgttta tactaaacaa aagaagatgc aggtaatgat cgtaacgaaa
    38221 catccagaaa aagtagtaaa tgaaatccaa aactgtatgc gtagagggat cacgatcatc
    38281 aatgaagcag aaggtgctta taagcatgat aagcagacag tattgatgac gatcgttaca
    38341 cgatacgaac tgcctctact tagagaagca atgaaagctt ctgatccaca agcttttgta
    38401 agtattgcag aaaacgtcca gatacttggt cgtttctatg aggatggcat ttagatcgag
    38461 tttgacaaaa attcatgaaa tactgctgta gacagacgtt cgtttgtctg cagttttttt
    38521 ctaccatagt agaatcagca aagaaaaact gcttttttat ttgaaatttt cttaaagttg
    38581 aaaaaaagca tgtttatttt tttaaattta tgggatgata gaaaaaaata gtagagaaag
    38641 gacacgattc tacttgctat tttcacttaa tacccgtata ttacgaatga aaaaagttta
    38701 tgaaaagttg gaatattgaa tggaaaaaca aaaaaggtta aaaaacagcc gctacatcac
    38761 tgggtttgat gggctccgtt cattagctgt ggtgggtgtt atcttctatc accttctgcc
    38821 aactagtatg aaaggcggct atttgggcgt tccgattttt ttcgtcgtat ctggttattt
    38881 gatcacggat ctgctgcggc aggaatggga acaaaatgga aaaatcaata tttggcaatt
    38941 ttatgtcaga agaatgaaga gactctatcc ggcacttgtt tttatgcttg tgacagcttc
    39001 ggcttatatc actttgtttc agcgagggct gttaaacaat cttcgaggag ttgtggtcag
    39061 cagcttactg tatgtcaaca actggtggca aatcaataac ggtttatctt atttcgatcg
    39121 atttgctaat gagtcaccgt ttacgcatat ctggtcttta gcagtagaag gtcaaaacta
    39181 tttggtttgg ccgatcttgt ttgtattgtt gatggtattt gttcgtaaaa gaaaatggat
    39241 cttttatact gtgttaggta catccttcct ctccgccatc ttgatgatga tcttgtatac
    39301 accaggaggc gatccaaccc gtgtttatta cggtaccgac actcgtctgt tttctatatg
    39361 gttgggaagt gcgcttgcgt ttgtttggcc aagtacgcgt ttaaagaaaa acatcccgaa
    39421 acaagcgaaa aatattctga atatcgctgg aggaatctcg cttttggcac tgattctttg
    39481 cttcttcttt ttagatgatc attacaattt catctattac ggaggaatgc tcctagtgag
    39541 tctgttatgc gtagtgctag ttgcagtgac agcacacccc ggagcaagtt tgaatcgatg
    39601 gctgacaaat ccagtattta cttggattgg caaacgaagc tatggcattt atctgtatca
    39661 gtttccagtt atgatttttt atgaagcaaa ggtgactgat attgccgatc atgttttgtt
    39721 gcatacttta gtggaaatca cattgattct aatcatcagt gaactttctt atcgatatgt
    39781 ggagcgtccg ctagcacgtt ttgattatgg tcgtacattc caagtgatga aaggatggtt
    39841 cagcaaaccg atcatcagta agagcaaacc atgggttgtt ccaggcacat tacttgtagc
    39901 aattgcactc gttggtttcg ttacggcacc gaaaaacgct gtaacagccg atcaaaaaca
    39961 actgcaggca aaaatcgctg aaagtaaaaa attagcagaa gaaaccagaa acaattcaga
    40021 aaacaccact agttcggttg accaagcgat ccttgataag tacgagttgt ccaaggcaca
    40081 agggaataag gcacaaaaat tagaactaac ggcatttggt gattcagtta tgttggatgc
    40141 tgcaacggat ctacaagaac tttatccaaa agtagtggta gatggagatg tcggacgaca
    40201 attatatgcc agtgtaccgt acattgaaaa attaaaggaa caagatttgt tgaaagacac
    40261 tgttctgatc ggactaggaa cgaatggctc attctcggaa gctcagtttg atagtgtgat
    40321 ggatgcaatc ggtgatcgaa aagtatactg ggtcaatgtc cgagtaccga cgaagcgctg
    40381 gcaaaatgac gtgaatgcga tgcttgaaca gatgacgaaa aaatacgaca atatgacgct
    40441 gattgattgg tacagctaca gtaatgacca tagtgactgg ttttatgatg atcaagtcca
    40501 tccaaatcct gaagggatga cacattacat tcacttagtc agcgaagcct tgctaggagg
    40561 aagttcaagt acgaacagca gtgagacaaa cgtgacagaa tgatagctac aaaaaacttc
    40621 aggcaaaaac atttgttttt gcctgaagtt tttttatatt taccgtgttt ttaacattca
    40681 tatagattca ggaagcaaaa cggttgagtg tgtctgtaaa taacgttctt tttgttttcg
    40741 ggtgagtttt ttgaatgcag cttctgcttt tgttgcttcg cttcttgaag aaaatacttc
    40801 tgcatggatc atcttaacag gtcttctttt ttgaagacga gtatatttag caccagtacc
    40861 attgttatgt tcctctaatc gtctagaaag atcggttgta tatccaccat aaaatgtctg
    40921 atctttacag aataatacgt aaaaataatg ttcatttgcc atagagcatc tcacgaatct
    40981 ctgcagtgta ttctccatcc atattgtaag tgatcaatgg tggagcgact ttgaatccgt
    41041 ctaatttgcc ttctttgatt ccttcaacta acaaaatatt tgcctctttt ccaggttttg
    41101 ggtaaacaaa ttggatcctt ttaggtgcaa ttcgatagtc ttccatgacc tgaatgattt
    41161 ctaagaaccg atcaggacga tggaccatag taaagcgtcc atttgttttt agtagcttac
    41221 ttgaaatatc gatcacttct tctaacgtcg tatgaatctc atgacgggcg attgccaggt
    41281 gtttattagg gttcgtttta tttgttggca gccctttgaa ataaggtgga ttacagacga
    41341 ctaagtcaca agaattatgt cgaacgacag ataagctgtc tttcagatcg atggtgtgga
    41401 cagtcatttg ttcatttaaa tggttaagcg cgatactacg tcttgccatg tctgctagac
    41461 gaggttgaag ttcgatagcg tcgatagccg cctgggttcg tttacttaaa aaaagtccaa
    41521 ctgcgccatt tcctgcacaa aggtcaacaa tcttcccttt tttaggtaca cgggaaaagt
    41581 gagcaagcag cacagcatct aaagaaaaag aaaacacttt ggaactttgg ataatcttga
    41641 tatcttctgc gtataattgg tcgattcgtt cgtcttcgtg aagcatggat tccctccaaa
    41701 atgaaaaaaa tagtcgtctg tattatacta tttttattaa aaagaaccaa ggtggcttgt
    41761 caaacctcta ttttttatgc atactatgac cataagaagg gaaaggaaga gactatgttt
    41821 tatcgattta tgcgcggggt agttcgagta ctattagcct tagttaatgg aaatgcccgc
    41881 tatgagaata aagaagtttt accgcaagat gagaattaca tactagttgc tccccatcgt
    41941 acttggtggg acccattata tctggcggta gctgcagcac ccaaggaatt cagctttatg
    42001 gcaaaagaag aactgttcaa aaatccagtt ctgcgattta tattaactcg atccaatgct
    42061 tttccagtta aaagagaaaa tccaggacca agcgtcatta aaacacctgt aaagattttg
    42121 aaatcgacaa atttgagttt gatcatgttt cccagtggga caagacatgc tacagaactt
    42181 aaaggcggaa tggcattgat ttccagaatg tctaaagcga aaatcgtgcc agctgtatat
    42241 caaggtccgt tgacacttgg ggatctgttc aagcgtcaga gagtgacagt gcgtttcggc
    42301 gaaccaatcg acttatcaga tatcaagaaa acggataaag aaggtatggc ggagatcgaa
    42361 cgacgaacac aagcagcttt tgacaaattg gacaaagaag tgaaccctga tttcaaatat
    42421 gaagtcaaaa aaaaataaaa tgataagttt atgcgtgggc ggtcttttga atctactgtc
    42481 ctgttcagtt cagacttttt caatgaaaac gagggagaga cgtcaagtct gccctctttt
    42541 tgcgttccaa tatgtaaaat agaagacagg tgtcatgtaa tggatcaaag tgttataata
    42601 attagaagga cattttttga ggtgattgaa tgcgtttttt acatacagcc gattggcata
    42661 tcggaaaaaa actacaaggt tatgacttga ttgaagacca gcgtcatgta ctatcttcta
    42721 tactagatat cgcgaaagaa gaaaaagtag aggcaatcgt gattgccgga gacttatatg
    42781 ataggagtgt cccagctgtt gatgccgtag aattatacaa tgaattgatg gtcgattgga
    42841 atttaaagga aaagatcccg atttttgcta tatcaggaaa tcacgatagc agtacgagac
    42901 tttcagcggg tacgccatgg ttcagttatt cgaactattt tttgcgaaca cagctagcag
    42961 aagcatttga accagtcgag tttaaaaacg tacaattttt ctttcttcct tactttgaac
    43021 caattgcagc gagattatat tttgaagatg acaatatccg aacgatcaag caagcggtag
    43081 ccaaggtgat agataaaatg caggagctat ttgattcaga aaaaaaacaa gtattgatta
    43141 gccatttttt tgtaatggga agtgtaaaat ctgattcgga aacgaaaatc gaagtaggcg
    43201 ggttggattc gatccctggt gaactactta caccgtttga ttatgtagca ctaggacatt
    43261 tgcataatca agctgctttg agacaggata atgctcgcta tagcggttcc ccattgaaat
    43321 tctctttatc tgagatcaac caaaaaaaag gcgtatggat cgttgacctg acaacaggcc
    43381 tggagttgaa ttttagggag atcgaacccc tgcatgatat tgaagaaata acagcgagtt
    43441 tcaaagaatt gctggatcct gccttttatc agtccgtcaa tcgggagaat tacatctata
    43501 tctatctcac tgatagaacg atcatcccga atatgatgaa ccagcttcgg caaatatatc
    43561 ctcgtatttt ggggattgaa cgggcaaacg gtcgagcaga acaaacaaag cgcaaacgaa
    43621 aagtactaga aatgaaagat cctcaaaaat tagcagaagc attttttagt gaaatgacgg
    43681 aagagcagct aactgcaaac caagaaaaat ggctcggaga aacgttgcaa gaaatcatgg
    43741 aaatggaatg atcaagaatg aaaccgaaaa aattacaact taaaaatttt ggtcctttta
    43801 tcaatgagac cgtagatttt tcacgtttga cagaagcacc gctttttttg atcagcggga
    43861 agacaggagc tggcaaaacg acgatttttg atgggatcac cttcgcactt tttggtgaaa
    43921 cttccggaag acttcgctct ggaaaagaga tgcgttcttt gtttgctaca ccagacgaag
    43981 aaacaagcgt gactttttct tttgaacatc agcagatgag ctatgaaatc gaacgaaaac
    44041 cagaacaagt tttagcaaaa cgaaaaggaa acggaacaaa aaaacaggca gccaaagtaa
    44101 cgttgaccat ttttgatggg aaaggaaaag aaataaaaca aatcacaaaa cgtacagaag
    44161 cagatcagtt gatcagagaa ctgatgaatc tcgatgcgaa acagttttct caaatcgttt
    44221 tactacccca aggagaattc cgtaattttt tggtttcatc aagcagtgat aaagagactg
    44281 ttttacgtca tttattcggt actcaatttt tccaacagtt caatgagcga ttaaaagaaa
    44341 aagcaaaaca acagcaacaa aaattggatc atttagagca agagctgcat cttctgcaaa
    44401 aaaggtttgt tttagtcaaa gaagaggaac cagcagcagg gtttgaagcc gttttagccc
    44461 aatggacaaa ccatcaggca atactcgaaa aacaaatcac ggaagaaaaa gaagctctaa
    44521 cggaacttca ggaaaaacaa aagcaactag aaaattccta ctatcgtttt gaaagcttga
    44581 aaaaaagtta tgaagaaaaa acgatattaa taaacaagca aaaggaatta gctgctcaaa
    44641 aagacgaaat agaggaaaag aaacgatgga ttgggtatta tgaatttacg caacgattag
    44701 tcgaaccaat ccagcgccta caagaaataa aagttgaaaa aatagaactg tcacaaaaaa
    44761 gtagtgagca aacaaagaaa ctagcgatga ctacgcagaa ttatgaagag tggcaaaaaa
    44821 atgaagcaaa ccgcgtacgc cgacaaacaa aaatcaaaga agctgctcaa aaactgcaaa
    44881 acaaacagat gatggtgcca attgtcgaat ccttacgtag taaaaaacaa gacgcagaac
    44941 aattaacaat ttcattggat aaagaagaat tgaatgccga gaagctgtat caaaatctcg
    45001 ctatctatca ggaaagaact cgaattcttc aagaaaattt actggatcag gaaaaactgc
    45061 aagaagatcg tcttgctttt ggacagttaa aatatctaga tgaaaagcag caagaaatag
    45121 aaaaacaaaa agcaaaagac actgggtcgc tcgcacagat aagagaagaa ctcaaaaaag
    45181 caactgaaga aataacaaaa aatagtcagc tattgaaaga acagacagaa caagcagaac
    45241 agctgaaaag ccaatgggca aagcagcaaa ttgctcgtct tcagcttttt ttgcgtccag
    45301 gggagccatg tcttgtttgt ggatcaacgg aacaccccat acaacaatta aaacacgaac
    45361 agccaaataa ggaagatatc ttaaaagtag aacaacaact ggcagaagct gagtctgctg
    45421 tagaaagaac tcagcaaatc attgccaaaa gtgaagcaca tgaaaataat cttcaacaac
    45481 aattatcaga tttagaacaa aaaatcatgg caacgaatat acaattggct gaacaagaag
    45541 aacacttgca acaggctttg aaaaaggcat ttcctgagat aacagaacaa aaaacaaaaa
    45601 caactcttgt agtaatcgaa caatctttag atagacgaaa aagcaagatg gccaatgcaa
    45661 gaatagagct ggaagaaata agacaacaag cagaaaagct cgaacaacaa ttagctgtct
    45721 acaaacagca gcagacagaa aaaaataatc agctccagca actgcaagga gaaatcaaaa
    45781 gtttagaaca acaattgcaa gatacggata gtaactgttt agaagaggag atacgacgac
    45841 ttgaaaaaca gttagaagaa gactcgaatc aactagctgt tgagcagaaa gcaggagtgc
    45901 aattgttgca agatcttcat acgcttcgga cacagcttga tcttcaacat gaacaaatag
    45961 aaaaaataga tgaaaaaata gaaaaggaac aagaaagaat agctaaaaag ttagaaagcc
    46021 agtcgtattt ttcaacaatt gaagaaatct gtgcatttgc acaacagcaa gcacaatata
    46081 aagaaaacca acgtacagtt acttcttatc atgaagagca attagtgaca gctgaccggc
    46141 tgcgtcaatt agaaaatatc cagctaactg agacattccc agatacagaa gagatcagcc
    46201 aacagttaga agaaataaga cgtatgatcg aaataaagca agaaaagata tacggtttac
    46261 aagaacaaaa aattagtaat caaaaaatct acgatgaatg tctttcgatt tatcacacaa
    46321 gccagacgaa attagatgag cttacgcaat tacagcagct ttcgcaaaca ttcaacggag
    46381 aaaatcctaa gaagactagt ttggaacggt atgtgcttca ggtttatctt caagaagtgt
    46441 tacaagtagc aaatgatcat ctccaacgtt taacgaaaaa tcgttatcaa tttgagctag
    46501 cggataccat tggcagttat cgagggaaaa ccggattaga aatcaatatc tatgatgatc
    46561 aggcaggtat gtctcgaagt gcgcataccc tgtctggcgg tgagagtttt attgcagcac
    46621 tttctttagc attagctctt gccgatgtga tccagacaca agctggtggt gtagcaatcg
    46681 atgcattgtt tattgatgaa ggatttgggt cattagatga agaagctttg gaaatggcaa
    46741 tggaagcttt agaaacgatc gaaagtgaag gaagaatgat cggcatcatt agtcatgtaa
    46801 gagaattgaa agaacgagta ttacaacaaa taagaattga tacagaaggt agcggacaaa
    46861 gcaaaatacg ctatcaactt ggataaaaag ggagattggt aactatgaaa tggagtattc
    46921 cagaaaaagt cattgcacgt ggacgtcaat acttgcaaga aggccgagtg ttatcggttg
    46981 tccctgacca agaagaaaac gtgtggcatg cagaagtctt gggcagtgaa ttgtatatgg
    47041 ttgatttgga tggtacagca aaagagatag attactgcca atgtccctat tgggaggaac
    47101 ataaatattg taagcataca gttgctgtgg aactttattt acggtcgaaa aatctatctc
    47161 gtatcatgaa aaaagaccag ccaatgctag aaccggtaaa agcaacaagt gagggagaaa
    47221 tcattgcaaa aggtttttct cgtctaaaaa cacaaaacaa tcctcaagcg gtgcaacctt
    47281 tgattattga ataccaagta gaaacgattg agactaatca gtatcaccca gagctttcta
    47341 ttttggcact ttacttgcgt atcggttcat tggcatcacc gaaaaaaaca tatatcgtaa
    47401 aaaacatcta tgatttttta caagcttaca cagaaaaaga aacttacact gttaataaac
    47461 agtaccagtt tcgtctagat aaacgggcgt ttcaaaaaga agatcaaaaa atactcacgc
    47521 atcttgcagg aagtgctcag acgcatcaat tattaggtac gaacggctta caagtcaaag
    47581 gaaaactaga taaaagatat ctgcttctcc cagtagaaca agcccgcttc ctgttagagc
    47641 agatgaatca gactacacgt ttgcatctgc agataaatga acaaaacgat caaggaatac
    47701 aattttccga tcaaaacaaa cctttatcat tcaaagtgga aaaatttgga gaagattatt
    47761 tgctgcaagc attgaatgat tttgattttt tctttatgca ttatcagtgg ggagttatcc
    47821 aagggacatt ttattctctg acgaaatatc aacaaacggt ttatcaaact tggaaacaat
    47881 tgattcgccg attggagaaa ccagaagtag tgttcaagaa aaaagaatta ccgtctttgt
    47941 ttaaagagat tatccctctg ctgtcagaga tcggcgacgt agctattgcc cctgcagtag
    48001 aagaggaagt agcagatatc cccgtcgaat ttattttctt tttccgaaaa gcaaaaggaa
    48061 aaatccaagc tcgagtcgat ttcgtctatg gcgaagtcat ctattcgact gacccgaagc
    48121 atgaggtctc gacagaacag gcttttcagg tgttacgtga cacagtagaa gagaaacgag
    48181 tacttgatct attcagaatg tatcgctacc agaaaaatga aactggttat gaacgtccat
    48241 tgccaagcgg ggaagaacta tatgcctttt tccgcacaga actatctgtc ttccgtcagt
    48301 ttggtgaagt cagacttgga agaaaattgc gagagttgta tttagatgcg cataaacatc
    48361 aaccgaaaat cgaagtaacc gagaccggtt catggctcga tattcgattc gatgtgacag
    48421 ggatagagga gcaagaaatc gatcatgtcc tgcaaagctt gctgagaaat gatgcttttt
    48481 atacattaga aaatggtcag gttctttctt ttgattcaga agaatttcaa caaacaagcc
    48541 aagtgttgca acaattacgg gaatcgatac gtacagaaga aggaacgatc catgtaccta
    48601 aaaatcaagg attgatcatt caaaatcaat tggaaaaaag caatgctact tttagtgaat
    48661 cgttccaaac gatggtacaa gatctcattc atccagaaag ataccaggca cagcttccca
    48721 aaggattgaa tgcaacgatg agagattatc aaaagcaagg ttttcgatgg ttgaagatgt
    48781 taggtcatta tcagtttggc ggtattttag ccgatgaaat gggacttggg aaaacactac
    48841 agacaattgc ctttttactt tcggaaaaag aagaacggaa aagcttctct gctttgatcg
    48901 tagcgcctgc gagcttaatt tacaactggc aggcagaggt gagaaaattc gccccttctt
    48961 taagtatcca agtaatcaat gggaataaaa aagaacgtga agaattactg gctaaagaca
    49021 cagatatccg agtgacttct tatgccagct taagacagga tctagctaat tatcagtcgc
    49081 aaaaaattga ttatttgatt cttgatgagg cacaaatggt caaaaatagc agtacgaaaa
    49141 ctgcacaagc attgagagaa ttagctgtac ctcagcggtt tgcactgagt gggacaccaa
    49201 tcgagaataa tttagaagaa ttgtggtcat tgtttgcaac gattatgcca ggatttttcc
    49261 cgactaaaac aaaattcagg gaacttgccg cagaagaaat tgcgcaaatg attcgaccgt
    49321 ttattttacg gcgagataaa caaactgtgt tgaaagactt gccggaaaaa acagagatga
    49381 atctatattc tgcacttaca gaagaacaaa aaactgttta tttagcttac ttgcgtcaaa
    49441 tgcgtgaaga aatcacatca atggattctg aagcattcaa gaaaaaccgg atcgggatac
    49501 ttgctggtct gacaagactg cgacaaattt gctgcgatcc tcgtctgttt attgaagatt
    49561 accaaggcgg ttccggcaag ctcgaacaag taaaagatct actggtagct gccaaagaga
    49621 acaaacgaag agttttgctg ttttctcaat ttacagggat gctgactatc ttgcaagaag
    49681 agttagccga gctagggatc tcgacatttt atttaagagg cagtacgaaa ccacaagatc
    49741 ggttaagtat ggtagacgca tttaatgctg gcgaaaaaga tgtttttctg atttccttaa
    49801 aggctggggg aactggattg aatctcacgg gagcagatac ggtcatcttg tatgaccttt
    49861 ggtggaatcc tgccattgaa gaacaagctg ctggacgtgc acatcggatc ggacaaaaaa
    49921 atgtggtaga agtgtggcgt atgatctctg aaggaacgat tgaggaacgc atggattcac
    49981 tccagcaaga aaaacgcgaa ctgttccaaa aagtgatcca aggtaatgaa gaacagttga
    50041 cgaaactgac tgaagaagac atccgcatga ttttaagtgt aggagaaatc gaggagtaag
    50101 agactcgaaa aagcgttcag taatgggtaa gaaagccttt ctaaagaagc gattgtttat
    50161 ttgatttaaa aaatagcaag gaggctggga ttccatttta tcccagcttc cttgctatca
    50221 tttttttatt tctattttta aaggttatac aaagattgtc ttcattgtct ctaacagctg
    50281 atcaaaagaa gaaatctcga tatcgcaaat tggagtctgt tcaattttgc gttctcgatg
    50341 gttgaaccaa agcgcttgcc agttagcact ttttgcacct agaacatcat tgtcgtaatt
    50401 atcgccaacg tataaggtct cttctggaag catatgaaat tctttctcag ctagctgaaa
    50461 aatctctttt tctggttttt ggaaacctgt tgcttgagaa atgagcatat tgcgggaagg
    50521 aatccaattg ttcaattgaa gttgcttcag tttttttgtc tgatggtcag tcggcccatt
    50581 tgtgataatt cctaatggga cattcttttc cttcaagaaa tcgagggttt ttttgacctc
    50641 ctcatgcaag cagatattat caagttcttc ctcgtaaatt ttttgaaata agaggccatc
    50701 ttcttccgta atatgagggt agtctaaatc ttttagtgat tggctgatcc gatgtgctcg
    50761 catatattct agtgtccaat ctcctgccat tacttttggg aagttttcat cactatgatg
    50821 gcggaaccgg atatatagtg gatgcatatc tgcatctgaa acaagcggaa cgactctttt
    50881 gaccgcgttt cgaaatggct gctgctgatc atacatcgta tcatcaacat caaacacaac
    50941 tgatttaatc aaaattttac acgcctttcg gttttcattg attttttcac aaatccttga
    51001 ctattgtaac agaaaaaatt gtctaaaaaa gctgataaat aaaggttttt cttactatat
    51061 ttctatttcc ttgacagaat aaggttttta tatttcagtt tttttcacta aaaatcgtga
    51121 aacacgtcaa taagtttgat gaacaaatta atcattatgg tagaagtcga ttgatttatt
    51181 ttaatgcaat ctctgtggaa ttttcgaaag atttattttt ttaatacagt ctcaatataa
    51241 ctattcaaac ataaaataac gttatttgat ttatttgttt atagtaaatt ttatttagta
    51301 atcattgctt taaaatacta cgcttatata acagagaaaa taagcgttat aataaagaac
    51361 agtattattt aaaatgacag aaaacattac tattaaagaa ataaaaaaca taaaaaatct
    51421 taagaaatcg gtttcattta gcttttgaga taaaataata gtacagtttt atacgctgtt
    51481 tcatatgttt agaaaatttg aattagattt gaaaactcta atttttaaat gataaaaaaa
    51541 gcgagaatgc tggttttact gtatattaac cttagataac tttcattttt ttaaaaaaat
    51601 tatgaaaaaa aggtagctat ttaaaagttg attatgtata atagaaacag atgggagatt
    51661 attatgatct tctgttacaa aaaagtgatt cttatcacga aaaggagtgg tgcacgttgt
    51721 taacattata tacttcccca agttgtactt cctgcagaaa ggctcgtgcg tggttgcaag
    51781 aacatcagat tccatttgtt gaacgtaata ttttttcaga accattgaac agttctgaat
    51841 taaaagcgat tttacaaatg acagaagacg gaacagaaga aatcatctcg acacgttcca
    51901 aagtattcca aaaactgaac atggatttag atgatctgcc gttacaagaa ttattggaac
    51961 ttgtgcaaaa caatcctgga ttgcttcgcc gtccaatcat gattgatgat aaacgtctgc
    52021 aagttggttt taatgaagat gaaattcgtc ggttcctacc gcgcgatgta cgacaattag
    52081 aattacgtca agcacaattg atggctggat tatagaagga taatacgaaa cttggaaaag
    52141 actattacaa agatagtttt tccaagtttt tttgttttta caaaaagtat tcttgattag
    52201 atgtataaaa aggtagtaga agatgatgat tgcacgaaaa atagagaggg aaaattagct
    52261 gaaaagtcgg ctatatagta ttcgtctgcc aaaaatgtta tagttaaata agatagcttg
    52321 aaaggttgaa aaatccgctg gttttattaa attcgcttaa cagcgagatt ttttttcttt
    52381 acatttaagc tgatttcgct acaatgaatc tatattaaac attgtggcaa agtgaggtgt
    52441 agcaaaatgg aaatggaaca tattaatgaa aatacgatcc gtgtactaat tggtcatgaa
    52501 gatttagagg aacggggcgt ttcatttttg gatttacttg gcaatcacaa agaaatcgaa
    52561 aatttctttt atagtatttt ggaagaagtt gatatcgaag aggagttccg aggaagcgaa
    52621 gctgtcactt tccaagtttt gccaaaagga gacggattag aactgtttat cagtaaaaat
    52681 cttccggatg aagaaatttc tcaatcagaa gattctactg ttgattcaga agatgtgagt
    52741 gactttcttc gtcagcagat tattggcagc gatccggagg aagaattatc tggaagcgcc
    52801 ctagaagaac aggaaagaca agcaactttt gcttttgaag attttgaaca agtgatccaa
    52861 cttgcaacag atgtcgaact agagtctgct tggactgatt tgtatttatt cgaagagcgc
    52921 tattacctag tcgttcactt ctggatggaa aatctcaacc aagcggatgt agagaatcag
    52981 attgctcgaa ttttggagtt tgctgaaaag agcgatcgta caccagaaac attgaatgaa
    53041 tatggacaat gtctgatggc gcgtaatgct atcgagcgaa cgaaatttta ttttgtataa
    53101 atcagcatta tagtaagaga ctgtgacaaa aattgtgtcg cagtcttttt ttcgtataaa
    53161 tgtgttcaaa agcttgcatc tgcggtaaca agcccaacca aacgaaaaat attgggaact
    53221 atttttgttt agctgttctt attacttgat gcagaatagc tttttcacgc cctctttttt
    53281 cgtgtaaata aattcttaga cgtaaataag ttcagaggga gaaaaatgct gcttgcaatg
    53341 aatgaatata ataagtgctg ttttgctaaa catgcagaaa aaggaaagaa atattattgt
    53401 cgtggctgtc gaaaaagagt gattttgaaa aagggaaaga aaaaatgtgc tcattttgcc
    53461 catcaaaaat cagataactg ttcggtcttt agtgaaagag aatcaaaaga gcacttacag
    53521 ttaaaggaat gcttcatgga ttggctggga cagtcagtag aaccagtatt tttggaagcc
    53581 tatttgccga ggttgcggca aaggccggat attttgttgg cgaatctagc aatagaaatc
    53641 caatgttcta gactgtctca ccagagattt attgaaagaa cccaaaatta tttgaataac
    53701 agttaccaag tatggtggat acttggtaat tcttttcttg gacaatcgca attctcattg
    53761 atagaaaaaa gttgctgcta ctataataga aaaagaggag tgcattgctg gaaagctgat
    53821 ctcaagaaac aaaagcttta tttgtatcat catattacag agactgtatc agggcatatt
    53881 tccttttgca gtagctgctg gactttttct agtagggacc ttaaagaaat attcactagt
    53941 aacgaaatta aaatgaacca aatgaaaaaa atagaaaaag tgagtgaaga tgggaagaaa
    54001 tggctgacac gccaacttat tcacaagcaa aagaatacag tatccataca agaacaatgc
    54061 tacttgcgtc ataaacatct tttgcatcta tcatcgtgga tctatcaaaa aagtcggttt
    54121 ttcttttatt tacaagaaca ggtttttctt tatcgaatgt tgtacgaaga agccttggaa
    54181 aaccagaaag ttcctgattt caactcatgg ctttgccaaa taaaagaaca taaaagggaa
    54241 tggctttttc ctatgattga tgaagagatg gtctatcagc agttttttaa cgaatgcata
    54301 cacttgtctt ctttgaaaaa atagtaagta taaaaatagg ctaggaatcg gttttctatg
    54361 taaaacaaga tttagttatg ctaaaattga tggaagcaga cacatgaatg agtagaaaag
    54421 ttagatgctg tgacatgtgg atgttagact atgatctatt ttgatagata gagctaaggg
    54481 aagaaaagga gtggatttca tggaagtaaa gcagttgcca aaacgagaag aattgcctga
    54541 aaatttaact tgggacttga ccaagatctt ttcaagcgac caagagtttg atgagaaata
    54601 tttggaatta tcagaagagt taaaacaatc tgaaaaacac aaaggaacac ttgatcaagg
    54661 cgcttctcaa tttttaaatg cgattgaatt cgtattgagg gtttatcgcc aaactgaagt
    54721 catttatgta tatgcgcacc ttaaaaacga tcaagacact ggaaatacag attaccaagc
    54781 gctttatgca agagcaagca gtctgttttc gaaagttagt gaagccgttt cctggtttga
    54841 accagaaata ttgcaattgt cagatgacca gatttggcaa tatttcaaag aagaaccaaa
    54901 attggaagtc tatcgccatt atatccagca aatagtagat aatcgagccc atgtcttatc
    54961 tgctgagcag gaatctcttc ttgctggagc aggtgaaatc tttgatgctt caagtgatac
    55021 atttgctgtt ttgaataatg cagatctagt ttttccaacg attgaaggag aaaatggtga
    55081 aatagtccaa ttatctcatg gcgtgtatgg tcagttgcta gaaagcacgg atcgaagggt
    55141 gcgcgaagca gcatttaagg ggttgtacag tgtttacgaa caatttagaa atacatttgc
    55201 ttctacttta ggcacacata taaaaggaca taattttaaa gcgaaagtcc gtaattacag
    55261 ctctgccaga gaagcgtctt tgagcaataa tcatattcct gaaagtgtat acgatacttt
    55321 ggtagacgtg gtaaacaagc atttgccttt gttacatcga tacatggaat tacggaaacg
    55381 tttattagaa gtggaaaaac tgcacatgta tgatctttat acaccggtct taggggaagc
    55441 tccaattacc tttacgtacg aagaagcaaa agaaaaagct ttagaagcac tgaaaccaat
    55501 gggtgaagaa tacatggcca tcgtagaaaa agcattctct gaacgttgga tcgatgttgt
    55561 cgaaaataaa gggaaacgaa gcggtgctta ttcttcggga agctatgaca caaatccata
    55621 tattttattg aattggcatg atacgctgga tcagctattt acgcttgtcc acgaaatggg
    55681 acatagtgtt catagttatt tcactcgttc gaaccagcct tatgtgtacg gcgactactc
    55741 catcttttta gcagaaattg cttcgacaac gaatgaaaat atcctaacgg agtatttatt
    55801 ggaaacagaa aaagaccctc gtgtacgggc ttatgtactc aaccattact tggatgggtt
    55861 taaaggaaca gttttccgcc agacacaatt cgctgaattc gagcatttca tgcatacgga
    55921 agatgaaaaa ggtgtgccat tgactagtga atacctaagt gatagttatg gtaaattgaa
    55981 tgcaaaatat tatggtccag cagtcgaaga agacccagaa attaaattcg aatggtcacg
    56041 gataccgcat ttttattata attactatgt tttccaatac tcgactggct tttctgctgc
    56101 ttcagcactg gcgaagaaaa tactaaacca ggaaccagag gcactagaga actacttggc
    56161 ttacctaaaa gcaggtaaca gcgattatcc tgtggaagta atgaaaaaag caggagttga
    56221 tatgacacag gctgcatata ttgaagatgc aatgtcgatg tttgaacaac gtctaaatga
    56281 attagaagaa ttgatcgatc gtttataagg ctttctattt ttgattttct cgaatagaaa
    56341 cgaaaaaaat acgtcggctc tcttgtaagg gagtcgacgt attttttta
//
LOCUS       DACGLE010000009        54930 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0534
            SAMEA3304449-rid9560453.denovo.009, whole genome shotgun sequence.
ACCESSION   DACGLE010000009 DACGLE010000000
VERSION     DACGLE010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304449
            Sequence Read Archive: ERR985064
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 54930)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 54930)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702225.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702225.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 20:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,045
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 27 of 67
            Pseudo Genes (incomplete)         :: 25 of 67
            Pseudo Genes (internal stop)      :: 19 of 67
            Pseudo Genes (multiple problems)  :: 4 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..54930
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304449-rid9560453.denovo.009"
                     /strain="VREN0534"
                     /db_xref="taxon:1352"
     gene            75..1072
                     /locus_tag="IXT20_000657"
                     /pseudo
     CDS             75..1072
                     /locus_tag="IXT20_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321168.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1312..1395)
                     /locus_tag="IXT20_000658"
     tRNA            complement(1312..1395)
                     /locus_tag="IXT20_000658"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1359..1361),aa:Leu,seq:gag)
     gene            1778..2995
                     /locus_tag="IXT20_000659"
     CDS             1778..2995
                     /locus_tag="IXT20_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAQ4859130.1"
                     /translation="MREFEKSNKLEGVSYDVRGPVLEEAERMQEEGISILKLNTGNPA
                     PFGFEAPNEVIRDLIMNARDSEGYSDSKGIFSARKAIEQYCQLKHFPNVTINDIYTGN
                     GVSELITMCMQGLLDNGDEVLVPMPDYPLWTASIALAGGTPVHYICDEEAEWYPDIDD
                     IKSKITSRTKAIVIINPNNPTGALYPKELLEEIVEVARQNNLIIYSDEIYDRLVMDGL
                     EHIPIATLAPDLFVVTLNGLSKSHRVAGFRVGWMVLSGDKSHVKGYIEGLNMLSSMRL
                     CSNVLSQQIIQTALGGYQSVDELLLPGGRIYEQREYIYNAINDIPGLSAVKPKAAFYI
                     FPKIDTKRFNILDDEKFVLDFLHEHHILLVHGGGFNWNQPDHFRIVYLPKMDDLKQTA
                     KKMREFLATYRQK"
     gene            3179..4015
                     /locus_tag="IXT20_000660"
     CDS             3179..4015
                     /locus_tag="IXT20_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ4859131.1"
                     /translation="METLIESFGTIIKEIRKEQKMTQKMLSHDICSQSVLSRIENNEE
                     LPNVLVMMQICQRLGVTIDHVMNLSRKTTRTNDKLFELLDSCFQKRDYRKLEQVLKSS
                     DISENLYQATDFQRYYYYLGVCEFTLRQNISQSLYYLKEALSYSSQKERIHVSDTEIQ
                     LISCIGKIYGVAGKTAEARYYLSRSIQLLHQSADERSKSEFSQIFYNYGNFLFHQNEI
                     DEALEQVNQGIYWAQEKNSYYYLNDLFVLKSLIYKRKEEPERAAFYEGLAQAVKQISK
                     NL"
     gene            4091..5239
                     /gene="nagA"
                     /locus_tag="IXT20_000661"
     CDS             4091..5239
                     /gene="nagA"
                     /locus_tag="IXT20_000661"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HAQ4859132.1"
                     /translation="MRTFIYADKFFLASGVKHAGYLEITDGLFGEYTKELPEGEVNII
                     DQSGKWIAPGLVDTHIHGYMNHDVMDNDAEGIKVMSEGLLSCGVTSFLPTTLTSSKER
                     LKDVAETIGKMYQEAPGAKIKGIYFEGPFFTEEHKGAQNPSYFGDPDLDTFHEWQEAS
                     GGLIKKIALAPERQGVEEFVKQVTEEGVVVSLGHSNATLEEAQKAVEAGASVFVHAFN
                     GMRGLNHREPGMVGALLSLEHVFSELICDGHHVHPNAAGILMEKAGHDHVALITDCMM
                     AGGMPDGNYNLGEFPVVVKEGTARLESGNLAGSILKLKEAIKNVVDWGLATPEQAVMM
                     ASLIPAISCKIDDTCGKIAQGRDADFIVLNPDMTLEATYLDGVERFHA"
     gene            5460..6518
                     /locus_tag="IXT20_000662"
     CDS             5460..6518
                     /locus_tag="IXT20_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014372913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HAQ4859133.1"
                     /translation="MNLFDIFRSTLTDMSIVSAITSTVFIILLGFVCRKKGIFSESVG
                     KILSKVVLTVALPALAFRAFMQDINTELLMQGINVLIWGLVIYIILILVSKPFFLSYK
                     GDKQTTLRVLTIFGSTTFFGTPIVSAIYGPVGVMYSSIFNIGYRIFLYSYGYIKMSGL
                     KMEAKNIKAMFVNPIIIATFAGLFIWLFQGYLPQVSVTGAEGEISQVAFLRIDQTAPW
                     LFQPMDYLAGLSSPLAWLAIGATLGEVSFKKAASEKTSWYYSLVKVVFVPALNIVILA
                     VLTATNILPVNFEALATIVIMMATPAATVAAAYAISFDREALLASNASLLSTIFAVVL
                     TPVWIVVLQMIHGIGLFS"
     gene            6537..7556
                     /locus_tag="IXT20_000663"
     CDS             6537..7556
                     /locus_tag="IXT20_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate dehydrogenase"
                     /protein_id="HAQ4859134.1"
                     /translation="MRIACYGVRPNEIDFFEKLNIYHYDLSLYEELLTHDNIVSAKDH
                     DAVLLRGNCIADETNLAKMQEYGIRYVFTRTVGVNHIDLQAAADFGMTVARVPSYSPN
                     AIAELSLTFAMMLLRNTAYTTIRTSFKDFRVDEQMFSREIRNCTVGIIGTGRIGLTEA
                     VLFKGLGAKVIGYDIHQTEAAKKVLTFHSLEELLAKSDIVSIHVPHIPGENDQMINEG
                     FLAQMKKGSILINTARGELQDNEAILRALKSNHLAGFATDVFANETEVFFRSFKPWET
                     IPDPAIQQLVEMYPRVLVTPHVGSNTDEALSNMISTSFENFREIIETGKTKNEVSLPK
                     ARQLR"
     gene            complement(7658..8374)
                     /locus_tag="IXT20_000664"
     CDS             complement(7658..8374)
                     /locus_tag="IXT20_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HAQ4859135.1"
                     /translation="MMTNVLIIEDDPMVAELNRTFVESIPNMNVIESFRNVEEGKKYF
                     RQKNADLLLLDVYMPGESGIHFLSWLRQQGNDIPVILITAADDIETIKNVIRYGALDY
                     LIKPFTLQRFQLAIEKYLAFSQTIQTTEKADQKLIDQVFNHTTPIHEIPSFIDLPKGL
                     SKLTLKKISAFILKEDTIFSTKELAQKSSLSRISVKKYLNFLVEQDILNERIKYLEIG
                     RPLTCYELTKNGRKKLTDYC"
     gene            complement(8343..9128)
                     /locus_tag="IXT20_000665"
     CDS             complement(8343..9128)
                     /locus_tag="IXT20_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HAQ4859136.1"
                     /translation="MYLNGIETISSVTPLFSNDRFLGAVATLRNRSEVMKLMEELGGT
                     KQYINSMRAQTHEFMNKLHVISGLIDLKKYEEVTHFIQQLNQNYKKEIGQVSSLIKVP
                     AIAGFLLGKINEAKEQSVEMLLESDSFIPKMEMDDSIHSLLQVLGNLLDNAKEAVLQK
                     HERNGQVRIHLNYDTEAEVFIVTVCDDGVGISEEISEKIFTLGFSTKGINRGFGLNLI
                     HSIVKRHGGFLEFSKQTNGGTIVYLELPKKEDQNDDKCPDNRR"
     gene            complement(9119..9868)
                     /locus_tag="IXT20_000666"
     CDS             complement(9119..9868)
                     /locus_tag="IXT20_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HAQ4859137.1"
                     /translation="MNRSSVSDKISSIAKIVANDKLIQKKLQTGDPTHAIQGITNEIM
                     IASNLDFIVVMDPEGIRYSHADPSYIGLPFSSHEDVKKVIGGQEFFSEKEGMLGFGFR
                     YFVPIYYDQEIIGAVCVGLTNNTIDNQVKDGQKMIVFSLIIGLSVGIIGAVFLAQKMK
                     KVLLGLEPQEIAEQLSEKTIIENEVDEGILAISSDKTILLINTPAKNIFYQTEQLLDF
                     EVGKKSVIIYMTFCLKRRLSIKRPFGLNPCI"
     gene            10166..10690
                     /locus_tag="IXT20_000667"
     CDS             10166..10690
                     /locus_tag="IXT20_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4859138.1"
                     /translation="MIGGIALETIYWYTLLICAGLAVLLIIFGDVFDFDGPLDPMLII
                     PWLTFTSLFGFLGERLTDQSSLFLFIASGGISIVLVFLLNFYILMPMKHAESTMSSSE
                     KTLEGQVAVVITPIPVRGMGEIQLKSVTGSISRPACFYSPQEQTAERGTKVLIIEIKE
                     RICYVMPYESMIKA"
     gene            10706..12205
                     /locus_tag="IXT20_000668"
     CDS             10706..12205
                     /locus_tag="IXT20_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HAQ4859139.1"
                     /translation="MEIPVVFIAIAIAALILLMLLIVFVAKYQTAKPDEALIISGSYL
                     GSKNVHIDESNNKIKIVRGGGAFVLPVFQRSNRISLLSSKLDVSTPEVYTEQGVPVMC
                     DGTSIIKIGSSVEEIATAAEQFLGKTREELENEAREVLEGHLRSILGSMTVEEIYQNR
                     DKFSQSVQEVASVDLAKMGLIIVSFTIKEVRDKNGYLDSLGKPRIAQVKRDADIAEAE
                     ALKETRIKKAQAEKESQAAELQRQTEIAESLKEKELKLATYKQEQDVAKAKADQAYNL
                     ESARAQQQVIEQEMQIKIVERQKQIELEEKEITRREKQYDSEVKKKADADRYAKEQEA
                     QAQKVKEVTEAEAERFRVEALAEAEANKTRLAGQAEAEAALAKGKAEAEAKQKIANAF
                     KEYGEAAVLSMVIDMLPQLMREAAQPLGNIEKISVVDTGSSSGETSGANRVTNYATNL
                     LSSTQETLKATTGLDLKEIVENLSTRGTTSQVNMYEAPKEAEQNSMKEE"
     gene            complement(12293..12895)
                     /locus_tag="IXT20_000669"
     CDS             complement(12293..12895)
                     /locus_tag="IXT20_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAQ4859140.1"
                     /translation="MQFGRFRLGMRTIKSALAVFLCILFFHLTQRGIPMIAALSAVFS
                     LRQDLTTTVSFGKSRIIGNTLGGTLAIIYFFVKSYFHNDFLVELILLPILVIIVIVVS
                     DGINNNSGIISAIATLLLIALSIPQGESALYAIQRVLDTFIGTFIAIGINFFLRPPET
                     EKKEELSEDLVELQKKERLLRTNLQEIEAKIQKQKKNSED"
     gene            12999..13955
                     /locus_tag="IXT20_000670"
     CDS             12999..13955
                     /locus_tag="IXT20_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HAQ4859141.1"
                     /translation="MRIGMRTVKTVISVFFSMVTAGSLSLLYWPAAGIIALLSVGNTK
                     RSTLRTGMNRIAAFILATATALFSFFAVGYTIFGFSLFLFLFIPLASRFKLTEGIVVN
                     SVLVTHYLVEENMSWQLIGNEAGLMAIGLVFALLANVYMPDRTKQLKENQIQIEKEFR
                     NILRQMAEFLLSENKEDVQIKCEHLLTFIRESQEDAREHQENYWLRQPLYYETYFSMR
                     RAQANVIKDMLENLERIQQPAYYGKHIYGLLIYTAETFSESNDGRQILTRIEEVYVLY
                     RQMPLPTSRPEFEDRAELFQFLQSFKSFIEIKAEFSQQKIQK"
     gene            complement(14260..15216)
                     /locus_tag="IXT20_000671"
     CDS             complement(14260..15216)
                     /locus_tag="IXT20_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAQ4859142.1"
                     /translation="MKKYQWGIVGLGTIAHEFAESFNQETSELTAVASRTSEKAENFA
                     HRYNIPKAYGSYQEMLDDAEIDIVYIAVPNRQHIDHILAALEAGKHVLCEKAITMNKK
                     ELADAMRLAEEKNLILAEAMTIFNMPLYQQLRSIMDTGKLGALKMIQAPFGSYKEPDP
                     KNRFFNPELAGGALLDIGTYAVSFARFFLSSQPEVVASTMVPFETGVDEQSVTILRNK
                     ENELAAVSLTFQAKMPKVGIVAFENGYVTIADYPRADRAEILFTDGTKEFIESGNTSE
                     AMNYEIKNMVHMIEGDLPNRSLFLTKDVIEILDQMQVLWKNM"
     gene            15390..15950
                     /locus_tag="IXT20_000672"
     CDS             15390..15950
                     /locus_tag="IXT20_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4859143.1"
                     /translation="MHQVYIDLIMSVLLEQFETEKNFLDEYLLISPEQWQKWKDGEKN
                     LPSEAMQKVKNLFTDYEWMLTQKILRQTIIFPEKRNVAVAEYKQIKTRIAQKWLSSKA
                     AKVELIPLKNNQQAGRYLDLKVSIQYDEWGYDDILNFRLPADVQKQIEGEKIELLDWV
                     NENLEETYVHEANGEREEQRKDFSSE"
     gene            15943..16767
                     /locus_tag="IXT20_000673"
     CDS             15943..16767
                     /locus_tag="IXT20_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HAQ4859144.1"
                     /translation="MNKFLGFGAVVIMIAAMGYVIFSDDDSHVQAGNKAVERTSTKTT
                     ASKEKNSQTTSSKVKDKETKDRTSKQLPDVKTDDWKLVLVGPDHKLEKEIDESTQLAS
                     LSNGYLIDKRIAPEYEAFAKAAEAAGFPLVMVSAYRSVSSQQQVFEQNVQDVMSRQGV
                     SEEEATKITKETITEPGYSEHHTGLAVDVVDQNWYNSYPSTVLDASYGDQPGAKWIAD
                     NAAQYGFIVRYPKGHEDITKITYEPWHLRYVGKENAEYITKHQLTLEEYLNELNEK"
     gene            16777..17394
                     /locus_tag="IXT20_000674"
     CDS             16777..17394
                     /locus_tag="IXT20_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HAQ4859145.1"
                     /translation="MAKKKWKKQKRKNIRWPAVVAGVAVILLAFVFSLKNLSSPFTDN
                     QQENEAQSHQQFIERLVPHAQELQDGYGVLPSIILGQAILESNWGKSTLASKYNNLFG
                     IKAYGDQKKVSLETKEFVNEEWITIQGDFKVYDSWEQSMDDHTQLFVQGVDWNPALYE
                     KVITATNYQEAAQALQDAGYATDPGYAQKIIQVIETYQLNQYDRR"
     gene            17412..18095
                     /locus_tag="IXT20_000675"
     CDS             17412..18095
                     /locus_tag="IXT20_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HAQ4859146.1"
                     /translation="MAEQFTPEIMTELRKDIVRVPEIILEASGIKIFGKLIRSIIFTT
                     DIAIIRNTNANAVIAVYPFTPHPAITKSIIEAADIPVFSGVGGGLTQGARSSYMSMFA
                     EAQGSLGVVLNGPTPLETVKEVCRVVDIPVISTVTSKYTEIDEKLAAGISIINISAGK
                     ETAEAVRHFRKKYPELPIIATGGPTEESIAETIQAGANAITYTPPSSGELFSRKMEKY
                     RETEKKNHE"
     gene            18778..20061
                     /locus_tag="IXT20_000676"
     CDS             18778..20061
                     /locus_tag="IXT20_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HAQ4859147.1"
                     /translation="MKKRMRIILALSVMLFFLTACGQEDDKKITLNMAWWGSQVRHDA
                     TVKVIELYEEKNPHVTIDYEFYDYEGYYTKLGTLAASDDIWDIFQLNNQFPQYINQIE
                     PLDEYVEQGIIDISNADESYLATTTYDGQLVGLSNGVNSFAIAYNKNIFEQLNIPEPA
                     SNWTWDEFEQISYQITEELGIYAISRFEDFIAGCIIQIPQVEKGLNFYNREDLSNSLG
                     FENPDYLTDFFQLRKNLVDKGAHPEPGGGAASTTDLSFQAVRDREAAMVFLSSNQLTE
                     ILSGAPEDMEIQLINPPRRKADGESGIPLRSSQMLSMAKSSAHKEEAAKFIDFFQNSE
                     EANEILKGERGVPIMSNIREQLARADDATLQATFDYLEMISDMDNGEVNVFDSALNPE
                     IQDQYNLLVEKVIYEEVTAEEAAESLYEFASNILG"
     gene            20077..20964
                     /locus_tag="IXT20_000677"
     CDS             20077..20964
                     /locus_tag="IXT20_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ4859148.1"
                     /translation="MKKEKLKRFLDQDNVVGYVFASPFILGFLLITVIPMILSLYYSF
                     TDYRLGNPVSWIGLDNYIKMLSDARFLNSIRVTFLYVFASVPVKLIFGLLIAYLLTRN
                     IKGTTFFRSLYYVPSLIGGSISIALVWRELFSKNGLVNIFLERLGAPALSWFSDQKLV
                     MIPLVLMSAWQFGSAMIIFAAGIKEIPGSYYEAAEIDGANKWQTFYKITLPLLSPIIL
                     YNLVMQTIAAFMTFTQAFIITQGGPNDATNFYALYVYNQAFSFNSMGYASAISWIMLL
                     IMGAVTFLIFKSSKKWVVV"
     gene            20981..21817
                     /locus_tag="IXT20_000678"
     CDS             20981..21817
                     /locus_tag="IXT20_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAQ4859149.1"
                     /translation="MNNTVTKWFQYGFILLLGLVMIYPVLWMIAGSFKTDQEIMSGSL
                     NLIPETFQWENYAKGWAGFAGISFFTFFRNSFVISAVSTIGTVLSSTCIAYALSRIDF
                     KGKRFWFVVMLVTMMIPAQVILIPQFIIYNRLNLVGTYVPLILPHFFGQAFFIYQIMQ
                     FMVSIPKELDESAIIDGCSKYSVFTKIIFPLLKPSIITTIIIQFYWKWDDFMGPLIYL
                     NKPRSYTVSIAIKLFADAGSSTTYASMFAMSTLSLLPVFLIFLFFNKYLVQGISTSGL
                     KG"
     gene            21820..22443
                     /locus_tag="IXT20_000679"
     CDS             21820..22443
                     /locus_tag="IXT20_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4859150.1"
                     /translation="MKKPIEEMFLYKLNYYVCRIFLTSLISNALFALVLGCLFTLGLV
                     LSARVVYLIYFLMMYPSYYLSVHYLAEKKVCWRKGKIKRMCFIITLFQLFVIIFAVDW
                     YLLVGKIGAWYLIPFLLCLLLFVSHSFFSTLIDICKEDKADSLVYLIKRNIWLLSKNV
                     KGFLWYLCLLYGLFWCIQHDFLLTALLYPGLPFVTVFCLKEKENDFK"
     gene            22440..23732
                     /locus_tag="IXT20_000680"
     CDS             22440..23732
                     /locus_tag="IXT20_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAQ4859151.1"
                     /translation="MKKKYVQVGLGGRARFFYEAIASTYHQTCELVGFCDVSQTRMDY
                     ANRILVNKYGQKKVKTCMPSQFEQLLDDVRPAEVIITSVDRTHHDYIIRAMKKGYDVI
                     CEKPLTIDEEKAQEIITVQKQTGRSLRVTFNYRYAPYHTKIKELIESDVIGDVFSIHF
                     EWLLNTQHGADYFRRWHRNKHNSGGLLVHKSTHHFDLVNFWLNSRPQKVFAFGGLNFY
                     GMHNAEKRGVEIFYSRCYGSELAKADPFAIDLSANEELREMYLEAEKDSGYIRDRSVF
                     SDDISIEDTLGVTVKYETGAILTYSLNAYMPWEGFNVSFNGSKGRIEMKVIEKSYINA
                     GGDKKDEGAAVLQSIHVYPMFGEPYEVIVEQEAGGHGGGDPRLLDDLFGEEKEEDPWN
                     RAATHVDGILSILTGIAANHSIASGKAVVIDELVSLKE"
     regulatory      24091..24188
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            24304..24885
                     /locus_tag="IXT20_000681"
     CDS             24304..24885
                     /locus_tag="IXT20_000681"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HAQ4859152.1"
                     /translation="MKELVERIQKDGRILGEGVLKVDSFVTHQVDPVLMEQMGKRFAE
                     VFKEQGITKVVTIEASGIAPALFAARELDVPMIFARKAKSLTMDEELLTASVYSFTKQ
                     VTSTISISRKFLSSEDKVLIIDDFLANGQAAKGLIELCQQAGAQVEGIGIVIEKSFQD
                     GRQLLEEMGLRVVSLARIASLTNGQVEFLEEDA"
     gene            24888..26195
                     /locus_tag="IXT20_000682"
     CDS             24888..26195
                     /locus_tag="IXT20_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HAQ4859153.1"
                     /translation="MEKETQNGKAAVLGLQHLLAMYAGAVAVPLLIGTGLGFNQEQMT
                     YLISIDIFMCGLATLLQLTVNRFFGIGLPVVLGCAIQAVAPLILIGTNEGVGAIYGSI
                     IASGIFVVLVSGVFSKIKKLFPPIVTGTVITVIGLTLIPVAIEKMGGGNATAADFGDK
                     TNLLLAFVTILLIVGVQMLAKGFIRSIAVLIGLVGGSILAAFMGMIDLSAIGDAPLFH
                     VPQPFYFGKPTFDVWSILLMIIISMVSMVESTGVYFALGDITGKNVGEEELKKGYRAE
                     GLAVILGGIFNTFPYTGFSQNVGLVQLSGIKTRKPIYFSAFFLIVLGLFPKIGALAQI
                     IPEPVLGGGMLVMFGMVAVQGIRMLLEVDFSNDKNLLIVAVSVGFGLGFNVTPTLFSQ
                     MPQTLQMFTGNGIVMSSITAIILNLIFNGLKKDEEILEEAFKK"
     gene            26611..27099
                     /gene="purE"
                     /locus_tag="IXT20_000683"
     CDS             26611..27099
                     /gene="purE"
                     /locus_tag="IXT20_000683"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HAQ4859154.1"
                     /translation="MTAKISVIMGSTSDWETMKEACQILDEFGVSYEKKVVSAHRTPD
                     LMFEYAQNARKKGIKIIIAGAGGAAHLPGMVASKTTLPVIGVPVQSRTLNGLDSLLSI
                     VQMPGGVPVATTAIGKAGAINAGLLAIQMLSMYDLELEAKLAERRSMLAKTVIESSDQ
                     LG"
     gene            27083..28216
                     /gene="purK"
                     /locus_tag="IXT20_000684"
     CDS             27083..28216
                     /gene="purK"
                     /locus_tag="IXT20_000684"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HAQ4859155.1"
                     /translation="MINLVKPLLPGSTIGIVGGGQLGRMMAISAKEMGFKVGVLDPVS
                     DCPAAQVADWHIESVYDDTFALKELARRTDVITYEFENVSVEALNAILPMSFIPQGTD
                     LLAITQDRLLEKSFLETNNIVIAPYATIVSPTDIQDAIDGIGYPCVLKTTRGGYDGKG
                     QYVLKSRADLAPAMDLLREGTCVLEAWIPFEKEISIMVAGNGQGDFTTFPVVENIHHN
                     NILHETIAPAAIDQDVIEEAERIARVIAEAVSLSGVLGVEMFLTKTGGLYVNELAPRP
                     HNSGHYSIEACSMSQFDAHIRGICGWPLGEKVRLISNAVMVNVLGEQLPESYRLIENK
                     PDWQFHFYGKKQAKKGRKMGHITILTEDIQQTLEEIKQTNIWD"
     gene            28229..29524
                     /gene="purB"
                     /locus_tag="IXT20_000685"
     CDS             28229..29524
                     /gene="purB"
                     /locus_tag="IXT20_000685"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HAQ4859156.1"
                     /translation="MLERYTRPEMGNIWTEENKYRAWLEVEILADEAWAELGEIPQED
                     VKKIREHASFDIDRILEIEKLTRHDVVAFTRAVSETLGEERKWVHYGLTSTDVVDTAY
                     GYLIKQANDILREDLKRFTEIIGEKAKEHKYTVMMGRTHGVHAEPTTFGLKLATWYSE
                     MKRNIERFEHAAKGVEAGKISGAVGTFANIPPFIEEYVCKQLGIRPQEISTQVLPRDL
                     HAEYFSAMALIATSIERFATEIRGLQKSETREVEEFFAKGQKGSSAMPHKRNPIGSEN
                     MAGLARVVRGHMVTAYENVTLWHERDISHSSAERIILPDTTILLNYMLNRFGNIVKNL
                     TVFPENMKRNMSATFGLIYSQRVMLKLIDKGMTREEAYDLVQPKTAYAWDNQVQFRPL
                     LEEDPTITSVLSKEEIDDAFDYHHHLKNVDVIFKRVGLE"
     gene            complement(29704..30081)
                     /locus_tag="IXT20_000686"
     CDS             complement(29704..30081)
                     /locus_tag="IXT20_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4859157.1"
                     /translation="MEDAIKQGQLFLSELTAEERSCLYNDLLSIKDVYLCNKDTSFYT
                     FRKIRRATKRCAECRKRTFLYQTEHKGELSISQIHEQKRFKNHYRIQILFNGQEMEYA
                     FLENLLQHLHNQHFSYNSQAIYA"
     gene            30337..31065
                     /locus_tag="IXT20_000687"
     CDS             30337..31065
                     /locus_tag="IXT20_000687"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HAQ4859158.1"
                     /translation="MEKKELLYEGKAKKIYLTSDENLIWIEYLDQATALNGARKDQIS
                     GKGELNNQITSAIFRYLTQRGIKNHFIEQLSKTEQLAKKVQIIPLEVVIRNIAAGSFS
                     KRLGVPEGKEFIKPIIEFYYKDDQLDDPFINEEHIYFLQLAKPEEISELKAQALAINE
                     ILTELFAKVGLVLVDFKLEFGRSNGQILLADEVTPDTCRLWDERTNEHMDKDVYRRDL
                     GDLIPVYQEVYNRLSELTEEKERV"
     gene            31065..31319
                     /gene="purS"
                     /locus_tag="IXT20_000688"
     CDS             31065..31319
                     /gene="purS"
                     /locus_tag="IXT20_000688"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010758866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HAQ4859159.1"
                     /translation="MYFVKVYVTYKDSVLDPQGEAVKKAVHRMGYETIEDIRIGKYFE
                     IQLADAEEPIDTVIETICDKLLANVNMETYRYEIKKMEEV"
     gene            31321..31992
                     /gene="purQ"
                     /locus_tag="IXT20_000689"
     CDS             31321..31992
                     /gene="purQ"
                     /locus_tag="IXT20_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HAQ4859160.1"
                     /translation="MKFAVIVFPGSNCDADMLWAVREIMGADAEFVRHDESSLEGFDG
                     VLLPGGFSYGDYLRCGAIARFSTIMEEVIRFAEEGKPVFGTCNGFQILTEAGLLPGVL
                     RRNESLHFICKTVPLKVNPKTAFTSEYEADEIIYLPVAHGEGNYFCDEETLQELKKNN
                     QIAFTYNDNINGSVENIAGITNKAGNVLGMMPHPERAVEDLLGSEDGKRFFASILKNF
                     GKVTV"
     gene            31993..34215
                     /gene="purL"
                     /locus_tag="IXT20_000690"
     CDS             31993..34215
                     /gene="purL"
                     /locus_tag="IXT20_000690"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HAQ4859161.1"
                     /translation="MKTLKEPTAEEIKKKRIYAEWGLTDEEYRMIEEDILGRMPNYTE
                     TGLFSVMWSEHCSYKNSKPVLRKFPTSGPHVLQGPGEGAGIVDIGDGLAVVFKAESHN
                     HPSAVEPYEGAATGVGGIIRDIFSMGARPIALLDSLRFGELDNPRTKYLLEEVVAGIG
                     GYGNCIGIPTVGGEIAFDPCYEGNPLVNAMCVGLIEHKHIQKGQAAGVGNSIMYVGAK
                     TGRDGIHGATFASEEFNQEEEQQRSAVQVGDPFMEKLLLEACLELILHHSDSLIGIQD
                     MGAAGLVSSSAEMASKAGSGLILTLDEVPQRETGMTPYEMMLSESQERMLICVKSGEE
                     EKIQELFQKYDLDAVTIGKVTDDGQYRLYHQGEEVANLPVDALAEDAPVYHKEMKEPA
                     RIVEFQQLAPYQPVIEEPGQVLLDLLQQPTIASKRSIYETYDSQVQTNTVVRPGSDAA
                     VMRVRGTNKALAMTTDCNARYLYLDPKIGGQIAVAEAARNIIASGGKPLAITDCLNYG
                     SPDKPEVFWELSTSADGIAAACETLGTPVISGNVSLYNETDGQAIYPTPMIGMVGLIE
                     DHKHITTQEFKKSGDLIYILGKTFADFDGSELQKMQLGRIEGVIRNFDLSIEKRNQEL
                     VLTAIQNGLIESAHDCSEGGLAIALAESAFKHQLGISVQFELSSAQLFAETQSRFVLT
                     VAPENKTRFEEMMGDAAVLAGKVTDEAIIEISATDGQIKIETAVARKCWEDAIVCLMK
                     "
     gene            34200..35639
                     /locus_tag="IXT20_000691"
     CDS             34200..35639
                     /locus_tag="IXT20_000691"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010768528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HAQ4859162.1"
                     /translation="MSYEVKSLNEECGIFGVWGHPDAASVAYFGLHSLQHRGQEGAGI
                     VSNDRGKLKGHRDLGLLSEVFKDQRKLQQLTGEAAIGHVRYATAGNGSVDNIQPFLFK
                     FHDQSIGLAHNGNLINARSLRKELEENGAIFHSNSDTEILMHLIRRSEETDFLNKLKS
                     ALNEVKGGFAYLLMTETAMIAALDPNGFRPLAIGQMKNGAYVIASETCALEVIGARFI
                     RDVQPGEIVIINDEGIQIDCFTKDTQLSICSMEYIYFARPDSNIAGINVHTARKNMGR
                     NLAKESPVKADMVVGVPNSSLSAASGYAEASGIPYEIGLVKNQYIARTFIQPTPELRE
                     QGVRMKLSAVRGVVEGKKVIMVDDSIVRGTTSRRIVHLLKEAGAKEVHVRIASPPLKY
                     PCFYGIDIQTRKELIAANHTIDEIKECIGADSLGFLSEEGLIESIGLEKDAPYSGLCM
                     AYFNGDYPTPLYDYEEKYQESLKEKVSFF"
     gene            35671..36714
                     /locus_tag="IXT20_000692"
     CDS             35671..36714
                     /locus_tag="IXT20_000692"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HAQ4859163.1"
                     /translation="MANAYAKAGVDVEAGYEVVERIKKHVKKTERLGMMGAIGGFGGC
                     FDLSVFQLKEPVLVSGTDGVGTKLAVAIEADKHDTIGIDCVAMCVNDILAQGAEPLYF
                     LDYLATGKNDPLRLENVVKGVADGCVQGNMALIGGETAEMPGMYSGNDYDLAGFAVGI
                     AEKSQLLTGETINPGDVLLGIASSGIHSNGYSLVRKIFFEQQQFSLSSKLPELKKTLG
                     EELLEPTKIYVSSLLPLIKEKKIKGAAHITGGGFVENIPRMLPESMAAQIKLGSWPIL
                     PIFGCLQKYGEIPEMEMYEIFNMGIGMVLAVEESKVKEIVHEIEQAGEKVYEIGKVIA
                     KEKHRVVFIGEER"
     gene            36711..37289
                     /gene="purN"
                     /locus_tag="IXT20_000693"
     CDS             36711..37289
                     /gene="purN"
                     /locus_tag="IXT20_000693"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HAQ4859164.1"
                     /translation="MRIAVFASGNGSNFQALADYLSKKGLESSIDWLFCDQPEAYVLK
                     RATALSVPADCFSPKEFDSKKEYEEAILHKLKEKKIDLIVLAGYMRIIGPVLLKNYDK
                     RIINIHPSLLPAFPGLHGIRDAFEAGVKETGVTIHYIDQGVDTGPIIRQEKVRIEQED
                     TFDSLEEKIHRVEHRIYPEVISEIIENGGYLK"
     gene            37286..38821
                     /gene="purH"
                     /locus_tag="IXT20_000694"
     CDS             37286..38821
                     /gene="purH"
                     /locus_tag="IXT20_000694"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HAQ4859165.1"
                     /translation="MTRALISVSDKTGIVAFAQGLIEQGVEIISTGGTKHALDKAGIK
                     TLSIEEVTGFPEMMDGRVKTLHPKIHGGLLGRRDLPSHMEAMETENIQPIDFVCVNLY
                     PFKETIMKPDVEIAEAIENIDIGGPSMLRSAAKNHEFVTSVVDPSDYEEVLSELRQYG
                     HTSLSFRQKLAAKVFRHTAAYDALIAQYLTDWVEEKEPEKLTLTYERKQTLRYGENSH
                     QAAAFYQSALLEKYSIAAAKQLHGKELSYNNIRDADAALRIMKEYDEPTVVALKHMNP
                     CGIGTASTILEAWEAAYEADPVSIFGGIIVLNREVDLPTAEQMHKLFLEIIIAPSYTQ
                     EALELLQKKKNIRLLTVDFAQEENQNREETVSVLGGLLVQDQDLAIENEEEWKVVTKR
                     KPTPAEAKALAFAWRAVKHVKSNAIVLANERQTVGIGAGQMNRVGSVKIAIEQAQEKM
                     EGAVLASDAYFPMDDSVEYAAKHGIKAIIQPGGSIKDQDSIDMANKYDLAMVFTDVRH
                     FRH"
     gene            38837..40111
                     /gene="purD"
                     /locus_tag="IXT20_000695"
     CDS             38837..40111
                     /gene="purD"
                     /locus_tag="IXT20_000695"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HAQ4859166.1"
                     /translation="MNILVIGAGGREHAISKKLLASPKVDTVYCAPGNPGMTKDGINC
                     VAISERDHSALIHFVKKEAIDWSIVGPEVPLLNGIVDDFMAEGLKIFGPTKAAALIEG
                     SKSFAKQLMDTYQIPTAKSRSFECYEKAAAYIKENGAPIVVKADGLAAGKGVVVATTV
                     KEALDAAEQMLNQHRFGDSSKKIVVEEFLAGEEFSLLAFVRNTKVYPMVISQDHKRAF
                     DNDKGPNTGGMGAYSPVPQISKKMIEKAVEEVLKPAALGMKELGRSFTGVLYAGLIAT
                     KEGPKVIEFNARFGDPETQVVLSRLKSDFAQVIDDLLENRTVELKWQQEGYNVGVVVA
                     AAGYPEEYETGMVLPDFLEEELSVYYAGVSSNNGKLVGAGGRLYLVEAYGETIKEAQK
                     KIYTALMDKTTDGTFYRTDIGEKAANSELIVK"
     gene            40233..41147
                     /locus_tag="IXT20_000696"
     CDS             40233..41147
                     /locus_tag="IXT20_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HAQ4859167.1"
                     /translation="MKKIQLGTSDQKAAAVILGCMRINNAEDPVKVIETAYENGIDFF
                     DHADIYGNGECEEIFADALAKTSIKREDLFIQTKCGIVPGVMFDFSKKHIIESVEKSL
                     TRLKMDYVDALLLHRPDTLVEPEEVAEAFDELERSGKVRYFGVSNQKPMQIELLKKYV
                     RQPLVANQLQFGLKHTGMIDQGIHVNMTDEASIDHDGSVLDYSRLHDMTIQAWSPYQY
                     GFFEGVFIGNEKFPELNQTLEELAAKYQTTPTGLASAWILRHPANMQVIAGTMNVGRI
                     KEIAQASEINMSREDWYELYRAAGNILP"
     gene            complement(41280..42845)
                     /locus_tag="IXT20_000697"
     CDS             complement(41280..42845)
                     /locus_tag="IXT20_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HAQ4859168.1"
                     /translation="MYKVFIIEDEYLIRNNLREQITVLGSYYPITYVGEAGDGEMALS
                     SIIDLQPDIILTDIQMPFMDGLTFSKEARKLLPWTRLIFISGFDDFDYMKGAIQVQAD
                     DYLLKPIKSSELKQAIEKAVTALDLQQKETHTSSDLVFELKKNHFLNGLFKGSLTISE
                     TLKLAESFNRSLAGKKMTVLLAANQVSTDFEDYAHLSDYLKFLFRENQQVIFSAVSSR
                     FIKFLIVDSLEENVIETSYQVAQTLVHELEKGQKNNFSVAIGPVAERISELPASFEIT
                     QMILETASSLKEQIISYEDLIQEAKLPVDSVFYFDLSLDLSSLSKNEVSKYIEHLTKP
                     QGTPTRTRLYRLFVLTKLLQHVCLKEKKIPQEMVHCTSTSQRLAISADDSLYRSTVYM
                     FISFLTALPSQPSRNKYQPILNHALDFIKENFTDPDMSLTWVAQQVALSPSYFSTIFS
                     QSFNQTFIDYLTEQRIELAKKLLQQSDYRISDIAFEIGYNDPNYFSYLFKKKQGISPK
                     EYRTQLFLKNKNN"
     gene            complement(42838..44289)
                     /locus_tag="IXT20_000698"
     CDS             complement(42838..44289)
                     /locus_tag="IXT20_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAQ4859169.1"
                     /translation="MTKNLSIKQIFAQANKAMLLFAVIPLLLSILLYSQQLLIYQQTI
                     ANIQKADAIALKVDQKVLESMWDLISGQVTVDEFNEENILEELRNDIREIKNNTNTLK
                     EISTLDVSLRTIDTLEDYQNELINNILAKDFMDTNEEIINQVDSVTQLLSDILQDFVR
                     VEIDLASKKNTTLVRSLILLTIVELGVLSTIFFYAKKNRRFLERKVQEPINQLIEMSE
                     QLSSGNLGFRLTLPETLELQTLTASMNKMAEDLNQSLRENALKQYHLAQSEVRVLQAQ
                     ITPHFIYNSLDAIISLIEQDCFEEALTMTHALSGFFRTSLSKGQDWIPLEKEISHIND
                     YLVILKIRYGHMLDYKIDIPETLFPVSILKMILQPLIENAVYHGTKYVRHKGFIRITT
                     CKIDEMIQFKVIDNGIGIPAEKLAEIRKELAKGIDSDFSIGYGLYNVNKRLLLYYGND
                     QKPITIDSKYREGTTVTLMIPLSPKTIGGNKHV"
     gene            complement(44303..45319)
                     /locus_tag="IXT20_000699"
     CDS             complement(44303..45319)
                     /locus_tag="IXT20_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAQ4859170.1"
                     /translation="MKRVLFFLALVVIAYQMMGCTPNTKEAPDPLVVGFSQSGTESSW
                     RKKHTESIITALEKEDYQVLYRNGYMNQERQIQDIRTFIAYKVDMIIFTPLQESGWDS
                     VLKEAKRAGIPVILVDRHIQTNDPELFVTHIGPSFKAEGNRAGLFVSNHFTNSKKQEI
                     RILELAGSENSTPTKLRSEGFLESINRKPTLKKDGTIDHKPPMTIVHRIVGDFIRMKG
                     KDQMKRYLETNDLSEIDVLYSHSDEMTHGALAAIKETSIKPGEDLIIVTIDGQEDMIE
                     KLRSGIVNCVVECKPDVGWYVANTVTRYFGNIQTGKTLPKDIFISETVFSQQNIANIP
                     TRNY"
     gene            45539..46627
                     /locus_tag="IXT20_000700"
     CDS             45539..46627
                     /locus_tag="IXT20_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HAQ4859171.1"
                     /translation="MKNKFRKIVGTIAILTASILLSACGNSGSADDSTGYVGIAMPTK
                     SAERWIADGNNMVSELEKLGYKTDLQYGEDKVENQVAQIENMITKGVDTLVIASIDGS
                     ALTDVLAKAKEADIKVIAYDRLLMNSENVDYYATFDNFGVGVSQAAYIEEHLGLKEGK
                     GPFTIELFGGSPDDNNALINYNGVMSVLQPYMDNGQLVVPSGQTSFSQIATLRWDGST
                     AQARMDNLLSANYTDQTLDAVLSPYDPISLGIISSLKGVGYGSESKPLPVITGQDATV
                     AGVKSIIAGEQTQTIFKDTRILAKNTIEMIKAISDGEEVPVNDTETYDNGVKTVPTYL
                     ANTVSVDKDNYQAELIDTDYYKESDLKN"
     gene            46681..48207
                     /locus_tag="IXT20_000701"
     CDS             46681..48207
                     /locus_tag="IXT20_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HAQ4859172.1"
                     /translation="MADCILKMADITKKFSGIRALDCVNLMIERGEIHALCGENGAGK
                     STLMNVLSGLYPYGSYDGEIFYDGKLCQFKDLKDSEEKGIVIIHQELALSPYLSIKEN
                     IFLGNEQAERGVIDWELTEKKTYELLNKVGLSIDPNLLVSQIGVGQQQLVEIAKALSK
                     SVRLLILDEPTAALNEEESANLLRLIRELKEQGVTSIIISHKLNEIVDIADRITILRD
                     GQTIETLENEMISEEHIIRGMVGRDLVDRYPERKPDIGEVYFKVEDWTVYHPLDHERI
                     INDHINLHLKKGEIVGIAGLMGAGRTEFAMSLFGHSYGSKISGRILKEGAEISVKTVP
                     SAIANGLAYVSEDRKSLGLNLLMDIRENVTLSSLDKISQKGVLDKEKEVTEAENFRKK
                     MRIKTNSIHQIVSSLSGGNQQKVVLSKWLMTQPDILFLDEPTRGIDVGAKYEIYTIIE
                     EMAANGKAVCLISSELPEILGMCDRIYTMNEGRITGEVSREDADQEVLMKLMTKEEVG
                     "
     gene            48209..49414
                     /locus_tag="IXT20_000702"
     CDS             48209..49414
                     /locus_tag="IXT20_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ4859173.1"
                     /translation="MEQAKNLTDKKEKKLNLKSRAADLFGRYSMVIILAGLLIAFQLM
                     TDGIFWRPLNITNLVLQNSHILVLAAGMLLVVLLGHVDLSVGSVMAFVGAIAGMMMVN
                     RQMNPWMTVIACLAVGGLIGAWQGFWVAYAGIPAFIVTLAGLLMFRGLTQVVLGGQSL
                     APFPAVFQKMSTGYLPDLSEGSSLHFLTLILGMIITIVLVVGQWRTRNRRKNHLFEVE
                     SPTAFLVKAVFTAVVVLGITYIFASYQGYPIILIILGLIVAAYGFLTNKTVAGRQIYA
                     TGGNRKAAELSGIKTKRITFWVFVNMGVMAALAGLVLAARLNAATPQAGTSMELDAMA
                     AVYFGGASTSGGIGTIMGAIVGGLVMGVLNNGMSILGVGVDWQQAIKGLILLLAVVLD
                     LYNKKKRTT"
     gene            49671..50648
                     /gene="guaC"
                     /locus_tag="IXT20_000703"
     CDS             49671..50648
                     /gene="guaC"
                     /locus_tag="IXT20_000703"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HAQ4859174.1"
                     /translation="MKVFDYEDIQLIPNKCIVNSRSECDTTVTLGKHTFKMPVVPANM
                     QTIIDETIAEFLAENGYFYIMHRFDEAARIPFIKKMKKRGLITSISVGVKKEEYSFIE
                     KLAEESLNPDYITIDIAHGHANSVIDMIQHIKKYLPETFVIAGNVGTPEAVRELENAG
                     ADATKVGIGPGKVCITKIKTGFGTGGWQLAALRWCAKAARKPIIADGGIRTHGDIAKS
                     VRFGATMVMIGSLFAGHEESPGETKVENGIVYKEYFGSASEFQKGEKRNVEGKKIWIQ
                     HKGSLKDTLVEMQQDLQSSISYAGGRDLEAIRKVDYVIVKNSIFNGDTI"
     gene            50912..52336
                     /locus_tag="IXT20_000704"
     CDS             50912..52336
                     /locus_tag="IXT20_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HAQ4859175.1"
                     /translation="MWEKVEKKQGPIYRQIMEQIMREIENGRINPGSQLESERKLGQL
                     FGVNRSTVVHALEELRELGVLTSKRGSGWFVNQTEWGKFAVPRIDWRQMISPRYEQTD
                     WYEQKIREAKQIQKTSFLDLYASEMPDELLPNFEFPSYSLEEILAEEKEMDLLGYTPL
                     RNKIKRYLESKFELAFSPNQLLVTGGGQQAIFLILQTILSAGEGIAVESPSFFYRLPL
                     FKASGTRLFGIPMDEEGIDLEKLKESIHKNKLKAVLINPNFQNPTGKVMSQKRREKLV
                     ELCRQYRLPIIEDDVFTDLSFLNQEEVLPIRTIDPDNVLYVGSLSRVLGKTTKIGWVI
                     GPETFISQLAQAQEMMEFSMNILTQITVANVFNEAIDLKMQDVRKKLKEKSRLISAWG
                     DSQSFFKLHSILGGYYAWITWDGKKLDPELAEKVVDMGLGIAPSLLFGQDINGIRINY
                     SRLDENQIPLFYSKMKILEKWLTE"
     gene            52797..53906
                     /gene="pdhA"
                     /locus_tag="IXT20_000705"
     CDS             52797..53906
                     /gene="pdhA"
                     /locus_tag="IXT20_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HAQ4859176.1"
                     /translation="MANKKAPIDFQALLDEVNADFPVYQALDQDGNVVNKDLVPDLSD
                     DELVELMTHMVWSRVLDQRSTALNRQGRLGFFAPTAGQEASQLASAYAFDKEDVLLPG
                     YRDVPQLVKHGLPLSQAFLWSRGHAAGNSYPEELKALPPQIIIGAQYVQAAGVALGLK
                     KRNKKNVVFTYTGDGGSSQGDFYEAINFAGAYHANAVFYIQNNGYAISTPREKQTAAK
                     TLAQKAVAAGIPGIQVDGMDPLAVYTVSKMAREWAISGNGPVLIETLTYRYGPHTLSG
                     DDPTRYRTKDTDDDWQKKDPLIRFRKYLTEKGLWSEEKEEQVIEATKEEIKAAIAEAD
                     KVPKQKVSDFLKNMFEVSPQSIKEQIAIYEAKESK"
     gene            53909..54886
                     /locus_tag="IXT20_000706"
     CDS             53909..54886
                     /locus_tag="IXT20_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HAQ4859177.1"
                     /translation="MAQKTMIQAITDALALELENDENVVVFGEDVGKNGGVFRATEGL
                     QEKFGEDRVFDTPLAESGIAGLSFGLALEGFRPVPEIQFFGFIFEAMDEVVAQMARTR
                     YRMSGTRNLPITIRSPFGGGVHTPELHSDNLEGLIAQSPGIRVVIPSNPYDAKGLLIS
                     SIRSNDPVVFLEHMKLYRSFREEVPDEAYEVPLDKAAVTREGTDVSIITYGAMVREAI
                     KAADNLAKENISVEIIDLRTVAPLDVETIIQSVEKTGRVVVVQEAQRQAGVAAQVVSE
                     ISERAILSLEAPIGRVSAPDTVFPFGQAENVWLPNAKDIEDKVKEIAEF"
BASE COUNT        17567 a         9381 c        11999 g        15983 t
ORIGIN      
        1 atgtttttga gaaaataaat gttaaaaatt tattattggt ttgctctata tactgtttaa
       61 taccattgac agtaatgggg gaaaccggtt ggagagcaac caccttgaac tatcagtggc
      121 cagtagcatt tagtttacta actttttatc cgttttttca actcttaaga ggagaagaaa
      181 taaatagaaa aatatattgg gtaagtattc ctttattgat atttttaact aatcaagaac
      241 aagtcaacgc ctgttttttt gttctaacaa gtatagttag tttatatttg attgtcaatg
      301 gacgatataa ttataagtta tcagtatttt caataataag cttagcagaa ttaattttct
      361 ctttgacaac tccagggaat gcattacgtg ctgcacatga aattaataaa tggtttcctg
      421 aatataaaaa tttcaacttt cttaataagt tagatttagg tatttcatca tttggaaagc
      481 cgttttttta gacatgaata tcttattttt attgctattt ttcttaattt tccttctaac
      541 atataggaaa tgtcaaaact attatgtgcg tatattgact gcattaccgt ttttcttaaa
      601 cctgattatt tattttggta atacaatggg tcaaagcttt acttacgtca atggaaataa
      661 aagggcaatg atttggagta gtagtaacct gaataatctc tttacagagt taggaacgaa
      721 actgtcactt ttttatccag gtacttggat agctacttta gtagtcttgg cattattact
      781 atgtttaatt gttggaattt atttaagttt tgacaataag aaaacttcta tttttttagt
      841 tattttgatg ataatgggat tttgttcaag gttaattatg ggattttctc ccacagtatg
      901 ggcttccgga atgcggacgt actatatact ttatgttgtt atcgccatct tagtactaat
      961 ggcagttaaa gagctaatga aaagcatgag tgtacaaaaa aatgaattta tgcaatttgg
     1021 attaaccgtg cttggtattt gtacctttat aataacggtg ataaacagat aatatacaaa
     1081 aaatccctcg ctttagtatt aggtgaggga ttttactcgt ttttcgatta attgttttaa
     1141 cacttgcaag tttctacgag tagcttgttt ccgaccttca tatactcacc tcaggctgtt
     1201 tacatgattg tacgttcttt ttgtaaagga tacagtgata tgtatataat ggcataaaga
     1261 aacgttcaaa caaaaaaact cgtgaagcaa aattaacttc acgagttgta gtgcagatga
     1321 tgggactcga acccacacga gctaacgctc acatgaccct caatcatgct tgtctgccaa
     1381 ttccaacaca tctgcgtaat aataacacac taatgatagc gaggttttac atagttgtca
     1441 agctcaaaaa tgggaggact tattaagatg gagaaatatg acggagaatt ttcaggactg
     1501 ggaatgatct taggtgtatt gattggactt gcattcggac gttttttgct tggttttatg
     1561 ctaggaatca tttgtggaat tgcaatggat tgggcagcga atctatggaa tgactatcat
     1621 gacaattaag ttctcaacgc ttgtttgtgt ttttttaata cttacgtttt tcctaaataa
     1681 gtggcaaaat tttctaggat ggagtatgat attgaaatag taaataacaa aaaatttgga
     1741 aagttctaac tttttgaaat gagggattgg ctaagtgatg agagaatttg aaaaatccaa
     1801 caaattagaa ggtgtcagtt acgatgttcg cggtcccgtt ctagaagaag cggagcggat
     1861 gcaagaagag ggcattagta ttttaaaatt gaacacggga aatcccgcac cttttggatt
     1921 tgaagcacca aacgaagtca ttcgtgattt gatcatgaat gcccgtgact cagaaggcta
     1981 ctcagattcc aaggggatct tttctgctag aaaagcaatt gagcaatatt gccaactgaa
     2041 acattttccg aatgtgacga tcaatgatat ctacaccggc aacggagtga gcgaattgat
     2101 cacgatgtgt atgcaggggt tgctggacaa tggagatgaa gtactggttc caatgccgga
     2161 ctatccgttg tggacggctt ctattgcatt agctggaggc acacctgttc attatatttg
     2221 cgatgaagag gcagaatggt atcctgatat tgatgatatc aaatcaaaaa taacgagtcg
     2281 gacaaaagct attgttatca ttaatcctaa taatcctact ggggcacttt atccaaaaga
     2341 attattagaa gaaatcgttg aagtagctcg acaaaataac ctgattattt attccgacga
     2401 aatctatgat cgcttagtga tggatggtct agaacatatt ccgattgcta cattagcgcc
     2461 tgatttgttc gttgttacgc tgaacggtct atccaaatct catcgtgtag caggatttcg
     2521 agtaggctgg atggtcctaa gtggagataa gtcacacgtc aaaggctata tagaagggct
     2581 gaatatgttg tcctccatgc gtttgtgttc caatgtattg tctcagcaaa ttatccaaac
     2641 cgcgctaggc ggttatcaaa gtgtcgatga gctgctcctg ccaggcggaa ggatctatga
     2701 acaaagagaa tatatctata atgcaatcaa tgatattcct ggtctttcag ccgtaaaacc
     2761 aaaagctgct ttttatatat tcccaaaaat tgatacaaag cgattcaata ttttggatga
     2821 tgaaaaattc gttttggact ttttacatga acatcatatt ctacttgtac atggtggtgg
     2881 atttaattgg aaccagccag atcattttcg aatcgtttat cttcctaaaa tggatgattt
     2941 gaaacaaaca gctaagaaaa tgagagaatt cctagcaacc tatcgccaaa aatgatttta
     3001 aaaaccaaat ttttcagtag gagggccaca gacaaacaca ctgaggctct ctttctagtg
     3061 gagttttctt aaataactaa agtatattat ttagttgcaa gtcttttctt gaagtttgcg
     3121 acttcctaaa gtaaaatacg aatagaatgc gaatacgcat attttgggga ggatacttat
     3181 ggaaacgtta atcgagtcct ttggaacgat cattaaagag attcgaaaag aacaaaaaat
     3241 gacgcaaaaa atgctttcgc atgatatttg ttcacaaagt gtattaagtc ggatcgaaaa
     3301 taatgaggaa ctgccgaatg tattggtgat gatgcagatt tgtcaacggc taggggtaac
     3361 gattgatcat gtgatgaatc tctctcgaaa aactactcgg acaaacgata aattatttga
     3421 actgttggat agttgtttcc aaaaacgtga ttatcggaag ttagaacaag tactaaaatc
     3481 ttctgatatt tctgaaaatc tgtatcaagc aactgacttt caacgctatt attattatct
     3541 cggtgtttgc gaattcacgt tgcgtcaaaa tatctctcag tccctttatt atttgaaaga
     3601 agcattaagc tactctagcc aaaaagaaag aatccatgtt tctgacacag aaatacaact
     3661 gattagttgt atcggaaaaa tatatggtgt ggcaggaaaa acggctgaag cacgctacta
     3721 tttgagtcga agcatccaac tgctgcatca atcagctgat gaacgatcaa aaagtgaatt
     3781 ttcgcaaatt ttttataatt atggaaattt cttattccat caaaatgaaa tcgacgaagc
     3841 gttagaacag gtgaatcaag gaatctattg ggctcaagag aagaatagct actattacct
     3901 caatgattta tttgtgctta aaagcttaat atacaagcgt aaagaagaac cagaaagagc
     3961 agcattctat gaaggcttag cacaagctgt gaaacaaatt tcaaaaaatt tgtaaatggt
     4021 gtagaccatt cttgacttct tctctaaata agagtacttt agaggtagaa aaacaaaggg
     4081 agagaataga atgagaactt ttatctatgc tgacaaattc tttttagcgt caggtgtcaa
     4141 acatgcaggc tatcttgaaa ttaccgatgg actttttggt gaatacacca aagaactacc
     4201 tgagggagaa gtcaacatca ttgaccaaag cggcaaatgg attgctcctg gtttggtaga
     4261 tacacatatt cacggctata tgaatcatga tgtaatggat aatgatgcag aaggcatcaa
     4321 agtgatgtct gaaggtctgt tatcttgtgg agtgacttca tttttaccaa caacattgac
     4381 gtcaagcaaa gaacgtttaa aagatgtagc tgagacaatc ggcaaaatgt atcaggaagc
     4441 accaggagct aaaatcaaag gaatctattt tgaaggtcct ttcttcacag aagaacacaa
     4501 aggggcacaa aacccaagtt atttcgggga tcctgatttg gatactttcc atgaatggca
     4561 agaagcttca ggtggtttaa tcaaaaaaat tgctttagca ccggaacgtc aaggtgtaga
     4621 agagtttgtc aaacaagtga ctgaagaagg agtagttgtt tctctaggac acagtaatgc
     4681 tacactagaa gaagcgcaaa aagctgttga agcaggcgca agtgtatttg tccatgcttt
     4741 caatggtatg cgtggattga atcatcgcga accaggtatg gtgggtgcgt tattatcttt
     4801 agaacatgtc ttttctgaat tgatttgtga tggccatcat gtacatccaa atgctgcggg
     4861 aatcctaatg gaaaaagcag gacatgatca tgtcgcatta attacagact gtatgatggc
     4921 cggtgggatg cctgatggaa attacaattt aggcgaattt ccagtagtag taaaagaagg
     4981 aacagcacgt ctagaatctg gcaacttagc tggaagtata ttgaaattga aagaagcaat
     5041 taaaaatgtt gtagactggg gattagcaac accagaacaa gcagtcatga tggctagtct
     5101 gattccagca atcagctgta aaatcgatga tacatgtgga aaaatcgctc aaggacgcga
     5161 tgcagacttt atcgtattga accctgatat gacattggaa gcaacttatc tagatggcgt
     5221 ggaacgtttc cacgcataat ataatttaag ttaaccttaa aactaaactt cttaagtttg
     5281 gttttaaggt tttttttgtg ttctagtaaa aatcacttta cataagtttc aaaagtttct
     5341 ttaatttcct attgttcaat aagtaacaat gtaaagataa aattaaaaat gttaatacgt
     5401 attagcaaaa taaaagcttt ttttattgtg aataaatgaa cataattagg aggagaaaga
     5461 tgaacttatt tgacattttt cgatcgactt tgacagatat gtcgattgtc agtgcaatca
     5521 cttcaacagt ttttattata ttattagggt ttgtttgtag aaaaaagggg attttttctg
     5581 aatctgttgg aaaaattcta tcgaaagtcg tcttaacagt ggctttgcca gcactggcat
     5641 ttcgagcttt catgcaagat atcaatacag aattactgat gcaaggaatc aatgtcttga
     5701 tttggggatt agttatttat attattctta ttctagtttc aaaacctttt ttcttaagct
     5761 ataaagggga taagcaaaca acgttgcgtg tcctgacgat ctttggttct acgacctttt
     5821 tcggaacgcc tatcgtcagt gcgatttatg gtcctgtcgg cgtgatgtat tcctcaattt
     5881 tcaatattgg ctaccgaatc tttttgtatt cttatggata cataaaaatg agtggcttga
     5941 agatggaagc taaaaatatc aaagcgatgt tcgtcaatcc aatcattatc gctacttttg
     6001 ccggcctttt catttggctg ttccaagggt atctgccaca agtgtcagtt acaggtgcag
     6061 aaggagagat aagccaagta gcgtttttgc gaattgacca gactgcaccg tggctatttc
     6121 agccgatgga ttatttagct ggtttaagtt caccattggc gtggctagca atcggcgcaa
     6181 cattaggtga agtaagcttc aaaaaagctg catctgaaaa aacttcatgg tattacagtc
     6241 tagtcaaagt ggtatttgta cctgcattga atatcgtgat tctagcagtt ttgacggcaa
     6301 cgaatattct accagtcaat tttgaagcac tggcaacaat cgttatcatg atggctacac
     6361 cagctgcaac agtggcagca gcctatgcta tcagttttga tagggaggca cttttagcct
     6421 ccaatgcatc cttactgtca actatttttg ctgtggtact gacacctgta tggatcgttg
     6481 tattacaaat gattcatggc ataggtttat tttcataaga aatggaggaa gaaagtatga
     6541 gaatcgcatg ttacggtgtt cgtccgaacg agatagattt ttttgaaaaa ttgaatattt
     6601 accattatga tctcagtctt tatgaagaac tgttgacaca tgacaatatc gtgtcagcaa
     6661 aggaccatga tgctgtattg ttgaggggaa attgtatagc agatgagacc aatcttgcaa
     6721 aaatgcagga atatgggatt cgttatgttt ttacacggac tgtgggagtc aatcatatcg
     6781 atctacaagc ggcagctgat tttggtatga cagtagcccg tgtaccatcg tattcgccaa
     6841 atgcgatagc agagttatct ttgacttttg ccatgatgct tttgagaaat actgcgtata
     6901 cgacgatccg aacatctttc aaagattttc gagtggatga gcaaatgttt agtcgtgaaa
     6961 tcagaaattg cacagtaggg atcataggaa caggcaggat agggttgacg gaagctgttt
     7021 tgtttaaggg actaggagcc aaagtgatcg gctacgatat tcatcagaca gaagctgcta
     7081 aaaaagtatt gacgttccac tcgcttgaag agttattagc caaaagcgat attgtcagta
     7141 ttcatgtacc tcatatccca ggtgaaaacg atcaaatgat caatgaagga tttttagctc
     7201 aaatgaaaaa aggtagcatc ttgattaata cagcccgtgg agagcttcaa gataatgaag
     7261 ctattttaag agctttgaaa agcaatcatt tagctggttt tgcaaccgat gtttttgcaa
     7321 atgaaacaga ggtttttttc cgatccttca agccatggga gacgattcca gaccctgcta
     7381 tccagcaatt agtagaaatg taccctagag ttcttgtaac cccgcatgta ggttcaaata
     7441 cagatgaggc attatcaaat atgatttcga caagtttcga gaactttcgt gaaataatag
     7501 aaactggaaa aacgaaaaat gaggtatctc ttcctaaagc ccgtcaatta agataagaaa
     7561 acatggatat tcgtcaataa aatgtttaaa aatagtccaa gtaaacagtt gttacttctg
     7621 tatacttgga ctattttgta tgggataatt cgatgactca acagtaatct gtgagctttt
     7681 tccgcccatt ctttgtcaat tcataacagg ttagcggccg gccgatttcc aaatatttga
     7741 ttctttcgtt caatatatct tgttctacta aaaaattcaa atatttcttg acggatatac
     7801 gtgacagtga gcttttttga gccagttctt ttgttgaaaa aatagtatct tcttttagaa
     7861 taaatgcact tatttttttt aaagtcagtt ttgacagacc tttaggcaaa tcgatgaatg
     7921 atggtatttc gtgaatggga gtggtgtggt tgaatacttg atcgatcaat ttctgatcgg
     7981 ctttttcagt cgtttggatt gtctgagaga aagcaagata cttttcgatc gccagctgaa
     8041 aacgttgcaa agtgaacggt ttgatcaaat aatctaaggc accgtagcgg atcacattct
     8101 ttatggtttc gatatcatct gcagcagtga tcaaaatcac tgggatatca tttccttgct
     8161 gtctaagcca agataaaaaa tggataccac tttcgccagg catgtataca tcaagcagca
     8221 gcagatctgc gtttttttgt ctgaaatatt tttttccttc ttctacattt ctgaaacttt
     8281 cgattacgtt catattaggg atgctttcca caaaagtacg attcaattct gcaaccattg
     8341 gatcatcttc gattatcagg acatttgtca tcattttgat cttccttttt tggtaattct
     8401 agatagacga ttgtaccgcc atttgtttgt ttggaaaatt ctaaaaatcc accgtggcgt
     8461 ttgacgattg aatggatgag attcaatcca aacccacgat tgattccctt cgtcgaaaaa
     8521 cctagcgtaa atattttttc tgagatttct tctgatatcc cgacaccgtc atcgcagacg
     8581 gtcacgatga atacttcagc ttctgtgtca tagtttaaat gaatccggac ctgtccgttt
     8641 ctttcatgtt tttgtagcac ggcttctttt gcattatcaa gtaagttccc taacacttgc
     8701 aaaagtgaat ggatggagtc atccatctcc attttaggga taaaagaatc gctttctaac
     8761 agcatttcaa cagattgttc tttggcttcg tttattttgc ctaataaaaa accggcaatt
     8821 gcaggtactt tgatcagact tgagacttgg ccaatctctt ttttataatt ttgatttagc
     8881 tgctggataa aatgggtgac ttcttcatac ttcttcaaat caatcaaacc agaaatgaca
     8941 tgaagtttat tcatgaattc atgggtttga gctctcatgg agtttatata ttgtttcgtc
     9001 cccccaagct cctccattaa tttcattact tctgatcgat ttcgtaaagt tgcgactgct
     9061 cctaaaaaac ggtcattgct aaaaagaggt gtaacgctag aaatagtttc tatcccattt
     9121 aaatacatgg attgagtccg aacggtcgtt tgatgctcaa tcgtctcttt aaacaaaacg
     9181 tcatatagat tatcactgat ttttttccta cttcgaaatc caatagctgc tctgtttggt
     9241 agaaaatgtt ttttgcaggt gtattgatca agaggatagt tttatcagaa gagatggcta
     9301 agattccttc atctacttca ttttcgataa tcgttttttc acttaactgt tctgctattt
     9361 cttgtggttc aagtccaagc agtacttttt tcattttttg ggcaagaaaa acggcgccta
     9421 tgatgcctac gctaagtcca ataatcaatg aaaaaacaat cattttttgt ccatctttga
     9481 cttgattgtc aatcgtgttg tttgttaagc caacacaaac tgcaccaata atctcttgat
     9541 cataataaat agggacgaaa tagcggaagc cgaaccctaa cattccttct ttttcagaga
     9601 aaaattcttg tcctccaatc acttttttca catcttcatg acttgagaaa ggaagaccga
     9661 tgtaacttgg gtctgcgtga gaatatcgaa tgccttcagg gtccatgaca acgataaaat
     9721 ctaaattcga tgcaatcatg atttcatttg ttattccttg aatcgcatgt gtcgggtcac
     9781 cagtctgcag ctttttttgg atgagtttat cattggcaac gattttagca atgctagaga
     9841 ttttatctga cacactagat cggttcatcg aaagaataaa attcctgatc aatatggcag
     9901 aaacaatcaa agaaacgaat gtggagaaac aaacgacaag gatgatcgtg ttctggagct
     9961 ttaatggttt tttcttaaaa aaaggaaaca taggctacct cgaaagaatt tattgtgaaa
    10021 attttaacat gaaaacaaaa atttaacaaa ttgtcaacga taaacacaac cttcctataa
    10081 aaaacaccac tgagtcggta aaagaagcta tacagaattt tcaaacattg gtatgataga
    10141 cgtagaaatg gaaggaggcg tgtatatgat agggggaatc gcacttgaga cgatttattg
    10201 gtatacattg ctgatttgtg caggattggc agtcctattg attatttttg gagatgtatt
    10261 cgattttgat ggcccgcttg atccgatgct tattatccct tggttaactt tcacaagctt
    10321 atttggtttt ctaggtgagc gtttaacaga tcaatcatct ttatttttat ttattgctag
    10381 tggaggcata tcgatagtgt tggtattttt gcttaatttc tatatattga tgccgatgaa
    10441 acatgccgaa tcaacgatgt cgtcttctga aaaaactctg gaaggacaag tagctgttgt
    10501 gatcacaccg atccctgtac gaggtatggg agaaattcag ttgaaaagtg ttaccggttc
    10561 cattagtcgt ccagcttgtt tttacagccc ccaagaacag acagctgagc ggggaacgaa
    10621 agtattgatc attgagatca aggaaagaat ctgttatgtc atgccttatg aaagcatgat
    10681 aaaagcttaa aggagcgtgt gttaaatgga aataccagtt gtttttatcg cgatcgcaat
    10741 cgcagctttg attttactga tgttgctgat cgtctttgta gcgaagtacc agacagcaaa
    10801 accggatgaa gcgttgatca tcagtggaag ttatttagga agcaaaaatg tgcacattga
    10861 tgaaagcaat aacaaaatca aaattgtgcg tggaggtggc gcattcgtct tgccagtttt
    10921 tcaacgttcg aatcggatca gtctattatc gagtaaattg gacgtctcca cccctgaagt
    10981 gtatacggaa caaggcgtac cggtgatgtg tgatggtact tcgatcatca aaatcggttc
    11041 atctgtagaa gagattgcta cagcagcaga acagttttta ggcaagacaa gagaagaact
    11101 ggaaaatgaa gcaagagaag tattagaagg acacttgcgc tcgattcttg gttctatgac
    11161 tgtagaagaa atctatcaaa atcgcgataa gttcagtcaa agcgtacaag aagtcgcttc
    11221 tgtagacctt gcaaaaatgg ggttgatcat cgtttctttc actatcaaag aggtcagaga
    11281 caaaaacggt tatcttgact ctttagggaa accaagaatc gcccaagtca aaagagatgc
    11341 agatatagca gaagcggaag ctctaaaaga aacgcgtatc aaaaaagctc aagcagaaaa
    11401 agaatcacaa gcagctgaat tacagcgaca aacagagatc gcagaatcat taaaagaaaa
    11461 agaactgaaa ttagcaacct acaaacaaga acaagatgtt gctaaagcaa aagcagacca
    11521 agcctacaat ttagaaagtg cccgagcaca acaacaagta atcgaacagg aaatgcagat
    11581 taaaatcgta gaacgtcaaa aacaaatcga actggaagaa aaagaaatca cacgtcgtga
    11641 aaagcaatat gattcggaag tgaagaaaaa agcagatgcc gatcggtacg ctaaagaaca
    11701 agaagcacaa gctcaaaaag tcaaagaagt gactgaagcc gaagcagaac gattccgtgt
    11761 ggaagcactg gcagaagcgg aagccaataa gacacgttta gctggacaag cagaagccga
    11821 agcagcacta gcaaaaggaa aagcagaagc cgaagcaaaa caaaagatcg ccaatgcctt
    11881 caaggaatat ggcgaagcag ctgtcttgag tatggttatc gatatgctac cgcaattgat
    11941 gcgcgaagca gcacagccgc taggaaatat cgaaaaaatc tctgttgtcg atacaggaag
    12001 cagttcagga gaaaccagtg gagcgaaccg agtgacaaat tatgccacaa atttgctttc
    12061 ttctactcaa gaaacactaa aagcgacaac aggacttgac ttgaaggaaa tcgtagagaa
    12121 tttgtcgaca cgtgggacta ctagccaagt aaatatgtat gaagcgccaa aagaggcaga
    12181 acaaaactcg atgaaggaag aataaagtag tacacttcac cgttcaatca aaaatggagg
    12241 aagacttttt aaagttcttc tctccatttt tttattttat tgcaaaagaa aattagtcct
    12301 cgctattttt cttttgtttt tggatcttag cttctatctc ttgtaaattt gttcttagca
    12361 atcgttcttt cttttggagt tcgaccaaat cttctgatag ttcttccttc ttttctgttt
    12421 caggaggacg aagaaagaaa ttgataccga ttgcaatgaa tgtccctata aaagtatcta
    12481 gtacacgttg gatagcatac aaagcggatt cgccttgagg gatgctcaac gcaatcagca
    12541 acaacgtagc tattgcggaa atgataccgg aattattgtt aataccatct gacacgacga
    12601 tcacaataat aactagaata ggaagcaaga tcaattctac caaaaaatca ttgtgaaaat
    12661 agcttttgac aaaaaaataa atgatagcta atgtaccacc taatgtattg ccgatgatcc
    12721 tagactttcc aaaagaaacg gttgtagtca agtcctgacg caaagaaaaa acagcagata
    12781 atgcagcaat catcgggatt cctcgttgag tcaaatgaaa aaacaagata cataaaaaaa
    12841 cagctaaggc acttttgatc gtacgcatac ctagtcgaaa acggccaaat tgcatagaga
    12901 ttctcctttt tggtcttttt actatcatac ctaaactttt gtctgatagc tagtttttca
    12961 ttattcaagt ataattaaaa taaaaaaggg ggaagtaaat gcgtatagga atgcgaacag
    13021 tcaaaacagt gatcagtgtc ttcttttcga tggtcacagc gggtagctta tcgctgttgt
    13081 attggccagc tgcagggatc attgcattat taagtgtagg aaataccaag cgctctacat
    13141 tgcgtacggg catgaatcga atcgcagcct ttatattagc tacggcaaca gcactgttca
    13201 gtttttttgc tgttgggtat actatttttg gcttcagtct ttttttattc ctgttcatcc
    13261 cgcttgcctc tcgcttcaaa ttaaccgaag ggatagtagt aaattcggtt ttggtcactc
    13321 attatttagt ggaggaaaac atgtcttggc agctgatcgg caatgaagcg ggattaatgg
    13381 cgattggatt agtatttgct ttgcttgcaa atgtttacat gccagatcgt acgaagcagc
    13441 tgaaggaaaa ccaaatacag atcgaaaaag aatttcgaaa tattttgaga caaatggcag
    13501 aatttctttt atcagaaaat aaagaagatg tgcagatcaa atgcgaacat ttactgacat
    13561 tcattcgcga aagccaagaa gatgccagag aacatcaaga aaactactgg ctcagacagc
    13621 cgctatacta cgaaacttac ttctctatga gaagagcaca ggccaatgtg attaaagaca
    13681 tgctggaaaa tctagagaga atccagcaac cagcttatta tggaaaacat atctatggtt
    13741 tattgattta tactgcagaa acattttcag agtcaaatga tggcagacaa attttaacac
    13801 gtattgaaga agtttatgta ttgtatcggc aaatgcctct gccgactagt cgccctgaat
    13861 tcgaggatcg agcagaattg ttccagtttt tgcagtcttt taaaagtttt attgaaatca
    13921 aggcagaatt ctcccaacaa aaaatccaaa aatagaggtt gtgaattcag ctaatattca
    13981 ggtcaaaaag ctgatttcac aacctcttct ttaaacggtg ttctcctgtc aactcctcgg
    14041 tcacaagtca caatattcaa caaacatgag aagctgtttt ttgaaattgc tccttgtgac
    14101 tcggagctga acggtaggct cacaacctct ttcttaaacg gtgttcaaaa gctttcctct
    14161 gcggtaataa gtccagccaa acgaaaaata ggaggagcta tttttgtttg gctgttctta
    14221 ttacttgatg cagaacagct ttttcacaac cttttaaaat tacatatttt tccatagaac
    14281 ttgcatttga tccaagattt cgatgacatc ttttgtcaag aagagagagc gattgggcaa
    14341 gtcaccttca atcatgtgta ccatattctt tatttcgtaa ttcattgctt cagatgtatt
    14401 cccgctttcg atgaattcct ttgtaccatc tgtaaataag atctccgcac gatcagcacg
    14461 cgggtaatca gcaatcgtca cataaccgtt ctcaaaggca acgataccga cttttggcat
    14521 ttttgcctga aaagtcaagc tgacagcagc aagttcattt tctttgttac gcaagatagt
    14581 gacggattgt tcgtccacgc ctgtttcaaa aggaaccatt gtagaggcaa ctacttcagg
    14641 ttgagagctc aaaaagaaac gagcgaaaga tacagcatac gtaccgatat ccaacagagc
    14701 tcctccagct aattctggat tgaagaaccg gtttttaggg tctggttctt tgtaactgcc
    14761 gaaaggtgct tggatcattt ttaaagcccc cagttttcct gtatccatta ttgagcgtaa
    14821 ctgctgataa agtggcatgt tgaagattgt catcgcttct gctaagatca aattcttttc
    14881 ttctgctaag cgcatagcgt cagccagttc ttttttattc atcgtgatgg ctttttcaca
    14941 tagaacgtgt ttgcctgctt ccaatgctgc caagatatgg tcgatatgct gtctatttgg
    15001 tacagcaatg tacacgatgt cgatttcagc atcgtctaac atttcttgat agcttccgta
    15061 agcttttgga atattgtaac ggtgagcgaa gttttccgct ttttctgaag ttctggaagc
    15121 aactgctgtt agttcgctcg tctcttgatt gaagctttcg gcaaactcat gtgcgatggt
    15181 accaagacca acgattcccc attgatattt tttcatctga aaacgcacct tcatagttgg
    15241 attgagtttc ttgtctgtct attattataa caaaaatcac gaagaataaa gaggagagga
    15301 cttgaaacat taaaaaaaat gaaagcagcg atttaagagg tgctttttga tcaaaactta
    15361 agtagaatag ttaaaaggag gggataaacg tgcatcaagt atatattgat cttattatga
    15421 gtgtattatt agaacagttt gaaactgaaa aaaatttttt agatgaatac ttgctgatca
    15481 gtccagaaca atggcaaaaa tggaaagatg gcgagaaaaa tctgccatct gaagcgatgc
    15541 agaaagtaaa aaatcttttc actgattatg aatggatgct aacgcaaaag attttgcgtc
    15601 aaacgattat ttttcccgaa aaacgaaacg ttgccgttgc agagtacaag caaatcaaaa
    15661 cacgcatcgc gcaaaaatgg cttagcagca aagcagcaaa agtcgagctg atccctttga
    15721 aaaataatca gcaggctggt agataccttg acttgaaagt aagtattcaa tatgatgaat
    15781 ggggctatga tgatattttg aattttcgtc tgccagcaga tgttcaaaaa cagatcgaag
    15841 gagaaaaaat cgaacttctc gattgggtga atgaaaatct ggaagaaact tacgttcatg
    15901 aggcgaatgg ggaacgtgaa gaacaacgaa aggatttttc aagtgaataa atttttagga
    15961 tttggagcag ttgtgattat gatcgcagca atgggctatg ttatttttag cgatgatgat
    16021 tcgcatgttc aagccggcaa taaagcagtc gaacgaacaa gcaccaaaac aactgctagt
    16081 aaagaaaaga acagtcaaac aaccagcagc aaggtaaagg acaaagaaac aaaagatcgt
    16141 acgtccaaac aattgccaga tgttaaaaca gacgattgga agctagtttt ggttggtcca
    16201 gatcacaaat tagaaaaaga aattgatgag tctactcaat tggcatcatt aagtaacgga
    16261 tacttgatcg acaaacgcat tgctccagaa tatgaagcat ttgccaaggc tgctgaagca
    16321 gctggatttc cattagtgat ggtctcagct taccggtctg tatcatccca acaacaagtt
    16381 ttcgaacaaa atgtccaaga tgtgatgagt cgtcaaggtg tatcagaaga ggaggcgaca
    16441 aagattacta aagaaacgat tacggagcca ggatacagcg aacaccacac aggtctggca
    16501 gtcgatgtcg tcgatcaaaa ctggtacaac agttatcctt ctactgtatt ggatgcaagt
    16561 tacggagatc agcctggagc taaatggatc gcagataatg ctgcgcagta tggatttatc
    16621 gttcgttatc caaaaggaca cgaagatata actaagatca catatgaacc ttggcactta
    16681 cgttatgtcg gtaaagaaaa cgctgagtat atcaccaaac accaattgac gctagaagaa
    16741 tatctgaatg aattaaatga aaagtaggaa ttgatcatgg cgaaaaaaaa gtggaaaaag
    16801 caaaagcgca agaatatccg ttggccagca gtggttgccg gtgtagcagt gatactctta
    16861 gcctttgttt tttccttgaa gaatctgtct tctcctttta ctgataatca gcaagaaaat
    16921 gaagcgcaat cccatcagca atttattgaa cggcttgttc cgcatgccca agaactacaa
    16981 gatggttatg gagtgcttcc tagtatcatt ttaggacaag ctatattaga atcaaattgg
    17041 ggaaaaagta ccttggctag caaatacaat aatcttttcg ggatcaaggc ttatggggat
    17101 cagaagaaag taagcctgga aacaaaggaa tttgttaacg aagaatggat aactatccaa
    17161 ggagacttca aagtgtatga ttcatgggaa cagtccatgg atgaccatac acaactgttc
    17221 gtccaaggag tagattggaa tcctgcgctg tatgaaaaag tgattacggc caccaactat
    17281 caggaagccg cacaagcatt gcaagatgca ggttatgcga cagaccccgg ttatgcgcaa
    17341 aaaatcatcc aggtcatcga gacgtatcaa ttgaatcagt atgaccggag atagcaaagg
    17401 agaagtggct gatggcagaa caatttacgc cagaaatcat gacggaattg agaaaagata
    17461 tcgtaagagt accggaaatc attttagaag caagcgggat caagatattt ggaaaattga
    17521 tccgttcgat catctttact acggatattg cgatcatacg taatacaaat gcgaatgcag
    17581 taattgctgt gtatcctttt acacctcatc cagcgattac taaaagcatt attgaggcag
    17641 ctgacattcc agttttctcc ggtgtaggtg gtggtttgac acaaggtgct cgttcttcct
    17701 acatgagcat gttcgcagaa gcgcaaggtt cgttaggggt cgttttgaac ggtcctacgc
    17761 ctttagagac agtaaaagaa gtttgtcgag tagttgatat tccagttatc agtacggtaa
    17821 cgtcgaaata tacagaaata gatgaaaagt tagcagctgg tatttcgatt atcaatatca
    17881 gtgcaggtaa agaaactgca gaagcagtac gccattttcg taaaaaatat cctgaacttc
    17941 ctatcattgc gacaggtggt ccaacggaag aaagtattgc tgagaccatt caagcaggag
    18001 caaatgccat cacatatacg ccaccaagta gcggagagct ttttagtcgt aaaatggaaa
    18061 aatatcggga aactgagaaa aagaatcatg aataacaggt gagaatcaaa agacgaggaa
    18121 gagcagatag tggctcttcc tcgtcttttg tactccagta ctatttgttc agaattcaaa
    18181 aaaaagagaa atttttttgt ttttttacct gtaatgtaca taagaagagt taaaaccttt
    18241 tgttattatt agcattctaa caaaaaaatg tcttgattta ttgaagcgct ttctatagaa
    18301 tgaaaaacga ataaaaacta aaaaaggcac ccgacaattt agtagcagct aaattgtcgg
    18361 ggcttgaaaa gtcagcggga actgaccaat caagttgcca tagtcaatgc ttgtgcatcg
    18421 acgtgtatat tgtacctaat ttcatacaga attactagtt atttttctgt tttttaggga
    18481 agagcgcaca cgaagaagca tgcttattta gctatgtcaa ggatattggg cagttgtctg
    18541 atatcctttt ttctttgcgt aattatcctc ccatcattga agcaagaagt gtttttagcg
    18601 atgaaagaag aatcagcact atctaatttt tcttgaaagt attgagttgt tcatcctagg
    18661 cagaacgata aggcaagtct tggtttttat tccccttgaa aaagatcgtt taataaatat
    18721 gttctgtata aaaatacagt atattgaaaa ataacaatgg aagtagaggt gcagagaatg
    18781 aagaaaagaa tgagaatcat cttagcttta agtgttatgt tattcttctt gacggcttgt
    18841 gggcaagaag atgacaaaaa aatcacgctg aacatggctt ggtggggttc gcaagtccga
    18901 cacgatgcaa cagtaaaagt aatcgagctt tatgaagaaa aaaatcccca tgtgacgatc
    18961 gattatgaat tttatgatta tgaaggatat tacacaaaat taggtacact agcagcatca
    19021 gatgatatct gggacatttt ccaactgaac aatcaatttc ctcaatacat caatcagatt
    19081 gaaccattag atgaatatgt ggagcaaggg ataattgata tttctaatgc agatgaatcc
    19141 tatttagcaa cgacgactta cgacgggcaa ttagttggac tgtctaacgg ggtgaattcg
    19201 tttgcgattg cttacaataa aaatatattt gaacaattaa atatccctga accagcttca
    19261 aattggacat gggatgaatt tgaacaaatc agctatcaaa tcactgaaga attaggaatc
    19321 tatgctattt caagattcga agattttata gctggctgta ttattcaaat accacaagtt
    19381 gagaaaggat tgaattttta taatcgggaa gatctatcta acagcttggg atttgaaaat
    19441 ccagactatc taacagattt tttccagctg cgtaaaaatc tggtagacaa aggggcccat
    19501 ccagaaccag gtggtggagc tgcaagtacg acggatctgt cttttcaggc ggttagggat
    19561 agagaagcgg caatggtgtt tctatcaagt aaccagttga cagaaatatt gtcaggggct
    19621 cctgaagata tggagattca gttgattaat ccacctcgca gaaaagcaga tggagaatct
    19681 ggtatccctt tacgttcttc gcaaatgctg agtatggcaa aaagctcagc ccataaagaa
    19741 gaagctgcta agtttattga tttctttcaa aacagtgaag aagccaatga aattttaaaa
    19801 ggagaacgtg gtgtaccaat catgtcgaat attcgagaac agctcgctcg tgcagatgat
    19861 gcaactttgc aagcgacatt tgattattta gaaatgattt ccgatatgga caacggcgaa
    19921 gtgaatgtct ttgatagtgc tttgaatccg gaaattcaag accaatacaa tcttttagtt
    19981 gaaaaagtca tttatgaaga agtaacagca gaagaagcag cagagagtct ttatgagttt
    20041 gcgtcaaaca ttctaggata gcagcaggag gaaggcatga aaaaagaaaa actaaaacgg
    20101 ttcttggatc aagacaatgt ggtcggttac gtatttgctt ctccattcat cctaggattt
    20161 ctattgataa cagtgatacc gatgatcttg tcactttatt attcgttcac cgattatcgt
    20221 ttgggaaatc cagtctcatg gatcggcttg gataattata taaagatgct gagtgatgcc
    20281 cgttttttga actccatccg agtcactttt ctctatgtat ttgcatctgt acctgtgaaa
    20341 ctgatctttg gtctattgat tgcttatctt ttaacgagga atatcaaagg aacgactttc
    20401 tttcgttctt tgtattatgt gcctagtttg atcggtggca gtatttctat cgctctagtt
    20461 tggcgggagt tgttttctaa aaacgggtta gtgaatattt tcctggaacg attaggcgca
    20521 cctgcgcttt cctggtttag cgatcaaaaa ctggtcatga tccctcttgt tttgatgtct
    20581 gcttggcaat tcggttcggc aatgatcatc tttgctgcag gtatcaaaga gatcccaggc
    20641 tcgtattatg aagcggcaga gatcgatgga gcgaacaaat ggcagacgtt ttacaagatc
    20701 acgttacctc ttctttcgcc gatcattttg tataacttgg ttatgcagac aatcgcggcg
    20761 tttatgactt ttacgcaggc atttatcatt acccaaggtg gtccgaatga tgccacgaac
    20821 ttttatgctc tttatgtgta taaccaagct ttttctttta attctatggg gtatgctagt
    20881 gctatttcgt ggatcatgct gttgattatg ggagctgtca cctttctgat tttcaaatca
    20941 tcaaaaaaat gggtcgttgt gtgaatgaga ggaggaagaa atgaataata cagtaacgaa
    21001 atggtttcaa tacggtttta ttttgctatt aggtctagtc atgatttatc cggtcttatg
    21061 gatgatcgca ggttctttta aaacggatca agagattatg agtggttcgc tgaatttgat
    21121 tccagagact tttcaatggg aaaattacgc aaaaggatgg gcggggtttg ctggaatttc
    21181 attttttact ttctttagga attcgtttgt catctctgca gtttcgacga tagggacagt
    21241 tctgagctcg acttgtattg cttacgcatt atcgagaatc gatttcaaag gaaaaagatt
    21301 ctggttcgtc gtcatgttag taacgatgat gattcctgca caagtgattt tgattccgca
    21361 gtttattatt tacaatcggc tgaatttggt tgggacgtat gtgccgctga ttttaccaca
    21421 tttttttgga caagcatttt tcatttacca gatcatgcaa ttcatggtca gcatccccaa
    21481 agagttagat gaatcagcga ttattgacgg atgcagcaag tatagtgtct ttaccaaaat
    21541 tatttttcct ttgctgaaac cttcgatcat cacgacgatc atcatccagt tttattggaa
    21601 atgggacgac ttcatgggac cattgattta tttaaacaag ccgagatctt acacggtatc
    21661 gattgccatc aaattatttg cagacgctgg atctagtacg acttacgcga gtatgtttgc
    21721 gatgtctacg ttgtcgctgc taccggtttt tctgattttc ttattcttca ataaatatct
    21781 tgtccagggg atcagtacaa gcggtctgaa gggctgaata tgaaaaagcc aatagaagag
    21841 atgtttcttt acaagctcaa ctactatgtc tgtcggattt ttttaaccag tcttatcagt
    21901 aatgctcttt tcgctttagt tctaggatgt ttgttcactt taggattagt gctgtctgct
    21961 cgtgttgtct atctgattta ttttttgatg atgtatcctt cttattatct atctgtccat
    22021 tacttggcag aaaaaaaggt atgttggagg aaaggaaaaa tcaaacggat gtgtttcatc
    22081 attacgttgt ttcagctgtt tgtcattatt tttgcagttg attggtattt attagtggga
    22141 aagattggag cttggtatct gattcctttt ctattgtgtc tgttgctttt tgtatcccat
    22201 agttttttta gtacgctaat tgatatctgc aaagaagata aagcagatag tcttgtttat
    22261 ttgatcaaaa ggaatatctg gctgttgtca aaaaacgtca aggggtttct gtggtattta
    22321 tgccttttgt atggactgtt ctggtgtata cagcatgatt ttttactcac tgcattactg
    22381 tatccagggc tgccatttgt cacagtattt tgcttaaaag aaaaggagaa tgactttaaa
    22441 tgaagaaaaa atatgtacaa gtcggacttg gtgggagagc gcgttttttt tatgaggcaa
    22501 ttgcctctac gtatcatcaa acttgtgaac tagtcggttt ttgtgatgta tctcaaacaa
    22561 ggatggatta tgcaaatcgg atacttgtga acaagtatgg acagaaaaaa gtaaagacat
    22621 gtatgccatc acaatttgag caactactgg atgacgtacg gccggctgaa gtcattatta
    22681 cgtcagtaga tcgaacgcat catgattata tcattcgtgc aatgaaaaaa ggatatgatg
    22741 ttatttgcga aaaacctctg actatcgatg aggaaaaagc tcaagaaata ataacggtcc
    22801 aaaaacagac gggaagaagc ctgcgagtca cttttaatta tcgttatgca ccgtatcaca
    22861 cgaagataaa ggaactgata gaaagtgatg tgattggtga tgtgttttct atccacttcg
    22921 aatggctgtt aaatacgcaa cacggggcag attatttcag aagatggcat cgaaataagc
    22981 acaatagcgg cgggcttctt gttcataaat ctacccatca ttttgatcta gtgaattttt
    23041 ggttgaacag ccgtccccaa aaggtatttg cattcggtgg attaaacttt tatgggatgc
    23101 ataatgcgga gaaacgcgga gtagaaatat tttacagtcg atgctatgga agcgaactag
    23161 ctaaagctga tccatttgct atagacctgt cagccaatga ggaattgcga gaaatgtatt
    23221 tagaagcaga aaaagacagt ggatatattc gtgaccgcag tgtattttcc gatgatatct
    23281 ctattgaaga tacactaggc gtaacggtca aatatgaaac tggtgcgatt ttgacgtatt
    23341 cgctaaacgc gtatatgccg tgggaaggat tcaatgtttc tttcaatggc agcaaaggac
    23401 ggatcgaaat gaaagtgatc gaaaagtctt acatcaatgc aggtggagat aaaaaggatg
    23461 aaggagcagc cgttcttcag tccatccatg tctatccgat gttcggtgag ccatatgagg
    23521 taatcgtcga acaagaagcg ggcggacatg gcggaggaga tccgcgttta ttagatgatc
    23581 tatttggtga agaaaaagaa gaagatccgt ggaatcgggc agcaactcac gtagatggta
    23641 ttttgtcgat tttgacagga atcgcagcaa atcattctat tgcttcagga aaagcagtag
    23701 tgattgacga gcttgtcagc ttaaaagaat aagaatagat ttacggaaat tacttcccgt
    23761 agtttataaa atcttgcctt attgccgata ggctgtcttt tgaacactat ttattcggaa
    23821 aaaggggcta tgacatgctt tttgtcatgc cttcttttta tgtgggctgc ttttttagat
    23881 aggagaaaat aaaaataact atttaaaccc gaacaaaaag gaataatgca ttaaaaatca
    23941 ttagatttta caaaaaaata agtaaaccac gttgtcagtt tcagaaagta gtgttagact
    24001 gttttcaata tttgaaacaa cattttttat tcaaatatgg caaactgcct tcacgtgcaa
    24061 aacagttgca agaaaatgaa tttacactat tattagtttt acatacctac ttatatatcg
    24121 tcataatatg gatgacagtt tctagccagt accgtaaatg ctggactata agtaaaagat
    24181 tggctatttc acccttcttt tcttatgata aagtggaggg tgtttttctt taacagaaac
    24241 atagtaggtt tttgcacaaa agcggttatt tcggtattaa taaatgataa aggagcaaat
    24301 aaagtgaaag aattagttga acgtattcaa aaagacggac gtatattagg cgagggcgta
    24361 ttaaaagtcg acagcttcgt cactcaccaa gtagaccctg tattgatgga acaaatggga
    24421 aaacggtttg cagaagtatt taaagaacaa ggaatcacga aagttgtgac cattgaagct
    24481 tctgggatcg caccagcttt atttgctgca agagaattag atgttccaat gatcttcgct
    24541 agaaaagcaa aaagtttgac gatggatgaa gaattattga cagcttctgt ttattctttt
    24601 acgaaacaag tgacaagtac gatttctatt tctcgtaaat ttttatcttc agaagacaaa
    24661 gtattgatta tcgacgattt cttggcaaac ggacaagcgg caaaaggatt gatcgaatta
    24721 tgccagcaag ctggagcgca agtagaagga atcgggatcg tcatcgaaaa gtctttccaa
    24781 gatggacgtc agttacttga agaaatggga ttgagagtcg tttctcttgc gcgtattgct
    24841 tccttgacga atggacaagt tgagtttctt gaggaggacg cataatcgtg gaaaaagaaa
    24901 cacaaaatgg aaaagctgcg gttttagggc tgcagcatct gctagcgatg tatgcaggag
    24961 cagtagcggt gcctcttttg atcggtacag gactaggctt taatcaagaa cagatgactt
    25021 atttgatttc aattgatatc tttatgtgtg gattagcgac attattgcaa ttgacagtta
    25081 accgattttt cggaattggc ttgcctgtcg ttttaggatg tgcgatccaa gcagtagctc
    25141 cactgatctt gatcggaacc aatgaaggag taggagctat ctacggatct attatcgctt
    25201 ctgggatctt tgttgtgtta gtttctggtg tcttttctaa aattaaaaaa ctattccctc
    25261 ctatagtaac aggaacggtt atcaccgtta ttggtttgac tttgattcct gtagcaatcg
    25321 agaaaatggg tggaggtaat gcaacagcag ctgattttgg tgacaaaaca aatctgctac
    25381 ttgcatttgt gacgattctc ctaattgtag gcgtgcaaat gctggcaaaa gggttcattc
    25441 gttcaatcgc ggtattgatt ggtttagtcg gaggaagcat cttagctgct ttcatgggaa
    25501 tgattgatct gagcgcgatt ggagatgcgc cgttgttcca tgttccacaa cccttttatt
    25561 ttggtaaacc aactttcgac gtatggtcga ttttactaat gatcatcatt tccatggtca
    25621 gcatggtaga atcaacaggt gtatattttg cacttggtga cattactggg aaaaatgttg
    25681 gagaagaaga attgaaaaag ggatatcgtg cagaaggact tgccgttatc ttaggcggta
    25741 ttttcaatac attcccttat actggctttt ctcaaaatgt cggattggtt caactttcgg
    25801 gaatcaaaac aagaaaaccc atctatttct ctgcattctt cttgattgtt ttaggattat
    25861 tccctaaaat cggtgcgtta gcacaaatca tccctgagcc ggtacttggt ggaggaatgt
    25921 tagtcatgtt cgggatggtg gcagtccaag gaatccgtat gctgttggaa gttgatttca
    25981 gcaatgataa aaatctattg attgttgctg tttctgttgg atttgggctg ggtttcaatg
    26041 ttacgccgac attattcagt caaatgccgc aaacattgca aatgttcact ggaaatggca
    26101 tcgtaatgag tagcattacg gctatcatat tgaatttgat ctttaatgga ttgaaaaaag
    26161 atgaagaaat attggaagaa gccttcaaga agtaatagag caaaagaaag gttgtgaaat
    26221 cagcgttttg acctgattgt tagagaaaca aacggaaaaa tagttcctcg tattttgccg
    26281 tttgtttgag ctaataccga gggtcagctg attgaacacc gttgaaggaa gaggtcgtga
    26341 gcctgccgtt catctccgag tcacaaggaa caatttcaaa aaaaagcttc tcatgtttgt
    26401 tgaatattgt tccttgtgac cgaggagttg gcaggagaac accgtttatc cataaaaagg
    26461 aatgtgacct aacttttgtc acattccttt tttttatctt taataaacac gaacaataaa
    26521 atgtttttct ttgttaacat tcgaaatttc tttgacatca aaaaaagtga ttgttaaaat
    26581 atgaatgtag aaaaactggg aggaaaaaca atgacagcaa aaatctctgt gattatgggg
    26641 agtacttcag attgggaaac aatgaaagaa gcatgtcaaa tattggatga attcggtgtg
    26701 tcttatgaaa aaaaggtcgt ttctgctcat cgaaccccag atttgatgtt tgaatatgca
    26761 caaaacgctc gaaaaaaagg aatcaaaatc attattgcag gagcaggggg agccgcgcat
    26821 ctgccgggta tggtcgcatc aaaaacaact ttgccggtta tcggtgtacc ggtccaatca
    26881 cgaacattaa atggtttgga ttcgcttctt tcgattgtcc aaatgcctgg aggagtacct
    26941 gtagctacta cagcaatcgg aaaagcggga gcaattaatg ctggactact tgcgatacag
    27001 atgctttcca tgtatgattt agaattggag gctaaattgg ctgaaagacg ttcaatgttg
    27061 gctaaaactg ttatagaaag tagtgatcaa cttggttaaa cctttgttgc ctggttcaac
    27121 gatcgggatc gtcggtggcg gtcaattagg tcgtatgatg gcgattagtg caaaagaaat
    27181 gggctttaaa gtaggtgtgc tggatcctgt gagcgattgt ccagccgcac aagttgcaga
    27241 ttggcacatt gaaagtgtct atgatgacac ctttgcgctg aaagagcttg ccagaagaac
    27301 agatgtcatt acctatgaat tcgaaaacgt cagtgtagag gcgttaaatg cgattttacc
    27361 catgtcattt attcctcaag ggacagactt gcttgcgatt acgcaagacc gcctgttaga
    27421 aaaatctttt ttggaaacga ataacatcgt gattgctcct tatgcaacga tcgtcagtcc
    27481 gacggatatc caagatgcga ttgacgggat cggctatcct tgtgttttga aaaccactcg
    27541 aggcggctat gatggcaagg ggcagtatgt tttgaaaagt cgggcggatc tagctcctgc
    27601 catggacctt ttaagagaag gaacctgtgt tttagaagca tggatcccgt ttgaaaaaga
    27661 gatttcgatc atggtggcag gaaatggtca aggagatttc acgacttttc cggtcgtgga
    27721 aaacatccac cacaacaata ttcttcatga aacgattgct ccagccgcga ttgatcagga
    27781 tgtgatcgag gaggctgagc gtattgcccg tgtgattgca gaagcagtga gcctttcagg
    27841 tgttttaggg gtagaaatgt ttttaacaaa aacaggcggg ctatatgtga atgaattagc
    27901 tcctcgtcct cacaattctg gacattattc tattgaagct tgttctatga gtcagttcga
    27961 tgcccatatt cgaggtattt gtggttggcc attaggagaa aaagtacgtt tgataagcaa
    28021 tgcagtaatg gtcaatgttt tgggagaaca actgccggaa agctatcgct tgattgagaa
    28081 taagccagac tggcaattcc atttctatgg aaaaaaacaa gcaaaaaaag gtcgcaagat
    28141 gggacatatc actatcttga cagaagacat ccagcaaacg ctggaagaaa tcaaacaaac
    28201 gaatatttgg gattagagga gaaaaaacat gttagaacgt tatacacgcc ctgaaatggg
    28261 gaatatctgg acggaagaga acaaataccg tgcgtggctc gaagtcgaga ttttagcaga
    28321 tgaagcttgg gcagaacttg gtgaaatccc gcaagaagat gtgaaaaaaa ttcgtgagca
    28381 tgcatcattt gatatcgatc ggatcttgga aatcgaaaaa ttaacacgcc atgatgtcgt
    28441 tgcgtttaca cgagcggttt ctgaaacatt gggagaagaa agaaaatggg tccattatgg
    28501 tctaacaagt acggatgtcg tagatactgc ttatggctat ctcatcaagc aagcaaatga
    28561 tattttacgg gaagacttga aacgttttac ggaaatcatt ggcgaaaaag caaaagaaca
    28621 caaatataca gtgatgatgg gtcggacaca cggtgtccat gctgaaccaa ctacttttgg
    28681 attgaaactt gccacatggt attcagaaat gaaacggaac atcgaacgat ttgaacatgc
    28741 ggcaaaagga gtagaagcag gaaaaatctc aggagcagtg ggaacgttcg caaatatccc
    28801 gccatttatt gaagagtatg tatgtaaaca attggggatc cgtccacaag aaatctctac
    28861 tcaagttctc cctcgggatc tacatgctga atatttctca gcgatggcgc ttattgctac
    28921 aagtattgaa cgttttgcaa cagagatacg cggattgcaa aaatccgaaa cacgtgaagt
    28981 agaagaattt tttgcgaaag gtcaaaaagg ttcttctgct atgccgcaca aaaggaatcc
    29041 gatcggttca gaaaatatgg ctggtctagc cagagtggtg cgcggacaca tggtcactgc
    29101 ctatgaaaat gtcacattat ggcatgaaag agatatttct cattcttcag cagaacgaat
    29161 catcttgcct gataccacga ttttattgaa ttacatgtta aatcgttttg gcaatatcgt
    29221 taaaaatcta acggtctttc ctgaaaacat gaagcggaat atgtctgcaa catttggttt
    29281 gatttatagc cagcgcgtta tgctgaaatt gatcgataag ggaatgacaa gagaagaagc
    29341 ttatgatctt gttcagccaa aaactgcgta cgcttgggac aaccaagtgc agttccgccc
    29401 gctgttagaa gaagatccaa ccatcacatc ggtattatct aaagaagaaa tagacgatgc
    29461 ctttgattat catcatcatt tgaaaaacgt tgacgtgata ttcaaacgag taggtttgga
    29521 ataaagaaga gtgagttgca aaattcaatt aaaatagaat agcaaaaata accaaaaaag
    29581 aagcagatcc gttagcggtt ctgcttcttt tagtttatta tattttctta tcaataatag
    29641 gaacctgatt ttaaaagcgg ctacctcttt accgtgcctc tttacataca aaaaatagag
    29701 gaattaagcg taaatggctt ggctattata actaaaatgc tggttatgga gatgttgcag
    29761 caagttttcg agaaaagcgt attccatctc ttgtccattg aataagatct ggatacgata
    29821 atgatttttg aaacgcttct gttcatgaat ctgactgatt gaaagttcgc ctttgtgttc
    29881 tgtctgataa agaaaagtcc ttttacggca ttcagcacag cgcttggttg ctcggcggat
    29941 ttttcggaat gtataaaaac tagtatcttt attgcataaa taaacgtctt tgatcgacag
    30001 caaatcatta tatagacagg aacgttcttc agctgttaat tctgataaaa aaagctgacc
    30061 ttgttttatt gcatcttcca tcggcaagtg cctcctcgaa cccattatcc aaatttttag
    30121 ttaattctaa tataccatca ttaagtataa atgtaaacgc ttgttgatgg tataagattt
    30181 tcgtttctga taaaacacga acattaaaag aatctaatgc cttaaatgta catatttttg
    30241 ttgatttata aaaagatgat tgctaaaatt taaagtgaaa aaacgaacaa aaaacgatgt
    30301 atttgctttt aatgtaaata aaaaggagag acgtacatgg agaagaaaga acttttgtat
    30361 gaaggaaaag ccaaaaaaat atatcttact tctgatgaga acttgatttg gatagaatat
    30421 ttagatcaag caactgcgtt gaatggcgcg aggaaagacc agataagtgg aaaaggagaa
    30481 ttgaataatc aaatcacaag tgcaattttc cgttatctga ctcaaagagg aataaaaaat
    30541 cattttattg aacaattgtc taaaaccgaa caattagcaa aaaaagtaca aattatccct
    30601 ctggaagtgg ttatccgaaa tattgcagca ggaagtttct caaaacgtct aggtgttccc
    30661 gaaggtaaag aattcatcaa accaatcatt gaattctact ataaggatga ccagttggat
    30721 gatccattta tcaatgaaga gcatatctat ttcttacaac ttgcaaagcc ggaagaaatc
    30781 agtgagctga aagcccaagc gttggctatt aatgaaattt tgaccgaatt atttgctaag
    30841 gtcggtttgg ttttagtaga tttcaagcta gaatttggcc ggtcgaatgg acaaattttg
    30901 cttgcggatg aagtaacacc ggatacttgc cgattatggg atgaacgtac gaatgaacat
    30961 atggacaaag acgtttaccg cagagatcta ggtgatttga tcccagtcta tcaagaagta
    31021 tataaccgtc tatcggaact aactgaagaa aaggagcgag tatgatgtat ttcgtaaaag
    31081 tatatgtaac ctataaagat tcagtgttag atcctcaagg ggaggcagta aaaaaagcag
    31141 tccatcgtat gggatatgaa acaatcgaag atatccgaat aggaaaatac tttgagatac
    31201 aacttgctga tgcagaagaa ccaatcgata cagtgattga aacgatttgt gacaagctgc
    31261 ttgcaaatgt aaatatggaa acataccgat atgaaatcaa aaaaatggag gaagtataac
    31321 atgaaatttg cggtcatcgt ttttccaggt tcaaattgtg acgcagatat gttatgggca
    31381 gttcgcgaga tcatgggcgc tgatgccgaa tttgtacggc atgatgagtc ctctttagaa
    31441 ggatttgatg gggttttact gccgggagga ttctcctatg gtgactattt gagatgcggt
    31501 gcgattgctc ggttttctac aatcatggaa gaagtgattc gttttgctga agaagggaaa
    31561 ccagtattcg gtacatgcaa tggattccag atattgacag aagcaggact tttgccaggt
    31621 gtactgagac gaaatgagtc tttgcatttt atttgcaaaa ctgtaccatt gaaagtcaac
    31681 cccaagacgg cctttacttc agagtatgaa gccgacgaaa taatctatct tccagttgct
    31741 catggggaag gaaattattt ctgtgacgaa gaaacacttc aagaactaaa aaaaaataat
    31801 cagattgcct ttacttataa tgacaacatc aacggcagcg tggaaaacat tgcagggatt
    31861 acgaacaaag cagggaatgt gttaggaatg atgcctcatc cagaacgtgc agtagaagac
    31921 ttgctaggat cagaagatgg gaaacggttc tttgcttcca ttttaaaaaa cttcggaaag
    31981 gtgaccgtat agatgaaaac attgaaagaa ccgacagctg aagagatcaa gaaaaaaaga
    32041 atctatgccg aatggggatt gactgatgaa gaatatcgaa tgatcgaaga agatatctta
    32101 ggaagaatgc ctaattatac ggaaactgga ctgttttctg ttatgtggag cgaacattgt
    32161 tcctataaaa attctaaacc ggttctacgc aaatttccta ctagtggacc tcatgtgttg
    32221 caaggccctg gagaaggcgc aggcatcgtc gacatcggtg atggactagc agttgtcttc
    32281 aaagcagaaa gtcacaatca tccttcagcc gtggagcctt atgaaggagc tgcgacagga
    32341 gttggcggaa tcatcagaga tattttcagc atgggggctc gtccaattgc gttgctagat
    32401 tcattgaggt tcggcgaatt agataatcca agaacgaaat atttactaga agaagtagtt
    32461 gcaggtatcg gcggatacgg caattgtatc ggcataccga ctgtaggcgg cgaaattgca
    32521 tttgatcctt gttatgaagg aaacccatta gtcaatgcaa tgtgcgtagg tttgattgaa
    32581 cacaaacata tccagaaagg gcaagccgct ggcgtcggta attcgatcat gtatgtaggt
    32641 gcaaaaacag gtcgcgacgg tatccacgga gcaacttttg cctcagaaga atttaatcaa
    32701 gaagaagaac agcaacgttc tgctgtacaa gtaggcgatc cttttatgga aaagctgctg
    32761 cttgaagcgt gtttggaatt aatcttgcat cattcggatt ctctaatagg tatacaggat
    32821 atgggagcag caggacttgt ttcatctagt gcggaaatgg cttcaaaagc tggctcagga
    32881 cttatattga cattagatga agtcccacaa agagaaactg ggatgacacc ttatgaaatg
    32941 atgttatcgg aatcacaaga acggatgttg atttgtgtta aatcaggaga ggaagaaaaa
    33001 atccaagaac tatttcagaa gtatgatcta gatgcagtca ctatcggtaa agtaacagac
    33061 gacgggcaat atcggctata tcatcaagga gaagaagtag caaatctgcc agtcgatgca
    33121 ttagctgaag atgcaccagt ttatcataaa gaaatgaaag aaccagctcg aatcgtagaa
    33181 tttcagcagt tagctcctta tcagccagtt atcgaagaac ctggacaagt attgcttgat
    33241 ctgctgcagc aaccgacgat tgcttcaaaa cgttcaatct acgaaactta tgattcacaa
    33301 gtacaaacca atacagttgt ccgtccagga agcgatgctg ctgtgatgag ggttagaggg
    33361 acgaataaag cgttagctat gaccacagac tgcaacgcgc gctatctgta tttggatcca
    33421 aaaatcggag gacaaatagc agtggcagaa gctgctagaa atattattgc tagcggtggt
    33481 aagcccttgg cgatcaccga ctgcctgaac tatggttctc ctgataaacc agaagttttt
    33541 tgggaattat cgacttcagc tgacggaatt gctgctgcgt gtgaaacgtt aggaactcct
    33601 gttatttcag gaaatgtctc tttatataat gaaacggatg gtcaagcaat ctatccaaca
    33661 ccaatgattg gtatggtcgg attgattgag gatcataaac atatcactac tcaggaattc
    33721 aaaaaatcag gggatctaat ctatatatta ggtaagacgt ttgcagattt tgatggcagt
    33781 gaattacaaa agatgcagct tggaagaatc gaaggtgtga tccgaaactt tgatctatcc
    33841 attgaaaaga gaaaccaaga gttagtattg acagccatcc aaaacggatt gattgaaagt
    33901 gctcatgact gttcagaagg tggcttggca attgcactgg cagaatcagc tttcaaacat
    33961 cagctaggga tatctgtaca gtttgaattg tcctctgctc aattatttgc agaaactcaa
    34021 tcaagatttg tgttgacagt cgcaccggaa aataagacac gattcgaaga aatgatggga
    34081 gatgctgctg ttttagcagg taaggtgacg gatgaagcga taatcgaaat ttcagcaact
    34141 gacggtcaga tcaaaataga aactgcagta gcaagaaagt gctgggagga tgcaatcgta
    34201 tgtcttatga agtgaagagt ttaaatgaag aatgcgggat cttcggtgtt tggggtcatc
    34261 cagacgccgc tagtgtggca tacttcgggc tccatagtct tcagcatcga ggacaagaag
    34321 gtgcaggtat cgtgtccaat gatcgaggaa agcttaaggg gcatcgagat ttggggctgc
    34381 tttctgaggt attcaaagat caaagaaaat tgcagcagtt aacaggagaa gctgcaatcg
    34441 gacatgtacg ttatgcaaca gcaggcaatg gaagtgtgga caatattcag ccttttctat
    34501 tcaaatttca tgatcagtcg atcggcttag cgcataatgg taacttgatc aatgctcgtt
    34561 cccttagaaa agagttagag gaaaatgggg cgatcttcca ttctaactcc gatacggaga
    34621 tattgatgca tttgatccga cgaagtgaag aaacagattt tttaaataaa ttaaaaagtg
    34681 ctttgaatga agtcaaaggt ggctttgcct atttattaat gacagaaacg gcaatgattg
    34741 ccgctttgga cccaaatggt tttcgtccat tagcaatcgg acaaatgaaa aacggagcat
    34801 acgtgattgc ctcggaaact tgtgcattag aagttattgg ggcacgtttt ataagagatg
    34861 tccagcctgg tgagatagtg atcatcaatg acgaaggaat ccagattgat tgctttacga
    34921 aggacacgca gctttctatt tgttcgatgg aatatatcta ttttgccaga ccagattcaa
    34981 acatcgcagg gatcaatgtg catactgcac ggaaaaatat gggacggaat ttagcgaaag
    35041 aatcgccagt aaaagcagat atggtcgttg gcgtacctaa ttcttctttg tcagccgcaa
    35101 gcggttatgc ggaagctagt ggaatccctt atgaaatcgg attagtaaaa aatcaataca
    35161 ttgcccgaac atttatccag ccaacgcctg agctaagaga gcaaggtgtt cgaatgaaat
    35221 tatctgctgt acgaggagta gtggaaggta aaaaggtgat tatggtcgat gattcaattg
    35281 ttcggggtac gacaagcaga cggatcgtcc atcttttgaa agaagcaggg gcaaaagaag
    35341 tccatgtgag gattgcttct cctcctttga aatatccatg tttttacggg attgatattc
    35401 aaacgcgtaa agaactcatt gcggcaaatc acacgattga tgaaatcaaa gaatgtatcg
    35461 gtgcagattc tttaggtttt ttaagtgaag agggtctgat cgaatcgatc ggtctggaaa
    35521 aagatgctcc gtattcagga ttgtgtatgg cttatttcaa cggagattat ccgacgccgt
    35581 tatatgacta tgaagaaaaa tatcaggaat cactaaaaga aaaagtatcg ttcttttaaa
    35641 agcagaccaa gaagggagta tatgaattca atggcaaatg catacgcaaa agctggcgta
    35701 gatgtcgaag ctggatatga agtagtagaa cggatcaaga agcatgtcaa aaaaacggaa
    35761 cgtttaggta tgatgggcgc tataggagga ttcggcggtt gttttgattt atcggtcttt
    35821 caattaaaag agccagtcct tgtctcgggg accgatggcg tggggacgaa attagcagtg
    35881 gcaatcgaag cagacaaaca cgatacaatc gggatcgact gcgtagcgat gtgtgtcaat
    35941 gatatcttag cacaaggcgc agagccgtta tatttcttag actacttagc aacggggaaa
    36001 aatgatccgt tacgtttgga aaatgtagta aaaggtgtag cagatggatg tgttcaagga
    36061 aatatggcgc tgattggcgg agaaacagct gaaatgcctg gtatgtactc aggtaatgat
    36121 tatgatctag ccggatttgc tgtagggata gctgaaaaat ctcaattatt gacaggagaa
    36181 acgatcaacc caggagatgt gttgcttgga atagcatcta gcgggattca ttcaaatggg
    36241 tactctcttg ttcgcaaaat tttctttgag cagcagcagt tttctttatc atcaaaactg
    36301 ccagaattga aaaaaacact tggcgaagaa ttgcttgagc caacgaaaat ctatgtttct
    36361 tccttgcttc cgctgatcaa agaaaagaaa ataaaaggag ctgcacatat cactggtgga
    36421 gggtttgtag aaaatatccc gcgaatgtta ccagaatcta tggctgcaca aatcaaactt
    36481 ggctcatggc cgattcttcc aatattcggc tgtttacaaa aatatggtga aattcctgag
    36541 atggagatgt atgaaatctt caatatgggc atcggtatgg tcctggcagt cgaagaatcg
    36601 aaagtaaaag aaatagtaca cgaaattgag caagctggcg aaaaagttta tgaaatcggg
    36661 aaagtgatcg ccaaagaaaa acatcgtgtg gtgtttatag gagaagaacg atgagaatcg
    36721 ctgtttttgc ttctggaaac ggaagcaact ttcaagcgct agccgattat ctatcgaaaa
    36781 aaggattgga atcttcgatt gactggttgt tttgtgatca gcctgaagca tatgttttga
    36841 aacgtgcaac tgcgttgagt gtaccggcag attgtttttc acctaaagaa tttgattcaa
    36901 aaaaagagta tgaagaagca attttgcata aattgaaaga aaaaaagatc gatttgatcg
    36961 tattagctgg atatatgcga atcattggac cggtattgct gaaaaattat gataaaagaa
    37021 tcatcaatat ccatccgtct cttttacctg ccttccctgg tttgcatggc atacgtgatg
    37081 cctttgaagc tggagtaaaa gaaacgggtg tcacaattca ttatatcgac caaggtgtag
    37141 atactggacc aatcatccgg caggaaaaag tgagaattga acaggaggac acgttcgatt
    37201 ctttagaaga aaaaatccat agagtagagc atcgaatcta tccagaagtg attagtgaaa
    37261 ttattgaaaa tggaggctat ctgaaatgac aagagcattg atcagtgtat cagataaaac
    37321 tgggattgta gcattcgccc aaggtttgat tgaacaagga gtagaaatca tttcaacagg
    37381 aggaacaaag catgcgttgg ataaagctgg gatcaaaacg ctttcgatcg aagaagtgac
    37441 agggtttcct gaaatgatgg atggccgtgt caaaacactt catcctaaaa tccatggtgg
    37501 tcttttagga agacgcgatc tccctagcca tatggaggca atggaaacgg aaaatatcca
    37561 accaatcgac tttgtttgtg tcaatttata tcctttcaaa gaaacgatta tgaaaccaga
    37621 tgtcgaaata gcagaagcta ttgaaaacat tgatatcggt ggtcctagta tgctccgtag
    37681 tgcagcaaaa aaccatgaat ttgtcacctc tgttgttgat ccatctgact atgaagaagt
    37741 actttcagaa ttacgtcagt atgggcatac ttcactttct ttcagacaaa aactagcggc
    37801 aaaagtcttt cgtcatactg cggcatatga tgcactgatc gctcaatact taacggattg
    37861 ggtggaagaa aaagaaccgg aaaaattgac acttacgtat gaacgaaaac aaacgcttcg
    37921 ttacggtgaa aatagccatc aagccgctgc cttttatcag tccgctcttc tggaaaaata
    37981 ttcgatcgca gcagcaaaac aactccatgg gaaagaactt tcttataata atatccgaga
    38041 tgcagatgct gcgctacgga tcatgaagga atatgacgag ccaacggtag tcgccttgaa
    38101 acacatgaat ccttgtggta ttgggacagc atctacgatt ttagaagcgt gggaagcagc
    38161 gtatgaagca gatcctgttt caatctttgg tgggatcatc gttttgaatc gagaagtaga
    38221 tctgccaaca gcagaacaga tgcataagct gtttctagaa atcatcattg ccccttctta
    38281 tacacaagaa gctttggaat tactgcaaaa aaagaaaaac atccgattac tgacagtaga
    38341 ctttgcgcag gaagaaaatc aaaatcgaga agaaacggtc tcagtcttag gtggacttct
    38401 tgtacaagat caagatctgg caatagaaaa tgaagaagag tggaaagtcg tgacaaaacg
    38461 taagccgact ccagcagaag caaaagcatt agcgtttgct tggagagctg tcaaacatgt
    38521 gaaatctaat gcgatcgtac tggcaaacga acgtcagaca gtaggaatcg gagccgggca
    38581 gatgaatcga gtcggttcag taaaaattgc gattgaacaa gcgcaagaga aaatggaagg
    38641 tgcagtttta gctagcgatg cttattttcc aatggatgat tcggttgaat atgcagccaa
    38701 acatggtata aaagcaatca ttcagccagg aggaagcatc aaagaccaag attctattga
    38761 tatggcgaat aaatacgatc tagcaatggt ctttactgat gttcggcatt ttagacatta
    38821 gagaggagtc ggataaatga acattttggt gatcggtgca ggtggtcgtg aacatgcaat
    38881 aagtaaaaaa cttttagcca gtccgaaggt ggatacggtg tattgtgcgc caggaaatcc
    38941 ggggatgaca aaagatggta tcaattgtgt cgctatcagt gagcgagatc attcagcgtt
    39001 aatccatttt gttaagaaag aggctattga ttggagtatc gttggtcctg aagttccttt
    39061 gttaaatggg atcgtcgatg attttatggc ggaaggattg aaaatcttcg gtccgacaaa
    39121 agcagcagct ctgatcgaag gttcgaaaag ttttgctaaa caattaatgg atacttatca
    39181 aatcccgaca gctaaatcca gaagttttga atgttacgaa aaagcagcag cgtatatcaa
    39241 agaaaatggt gcgccaatcg tagtgaaagc ggacggctta gcagctggga aaggtgtagt
    39301 cgtggctacc acagtgaagg aagcgctgga tgcggcagaa caaatgttga accagcatcg
    39361 atttggggat agtagcaaaa aaatcgtcgt agaagagttt ttggcaggag aagaattctc
    39421 ccttttggcg tttgtccgta ataccaaggt gtatccgatg gtcatttcgc aagatcataa
    39481 gcgggcattt gataacgata aagggccaaa tacaggcgga atgggtgctt attcaccagt
    39541 accgcaaatt tcaaaaaaga tgattgaaaa agctgtggaa gaagttttaa aaccagcggc
    39601 tttgggaatg aaagaactag gacgctcttt cacgggtgtt ttatatgcgg gtttgattgc
    39661 tacgaaggaa ggaccaaaag tgatcgaatt caatgcgcgt ttcggtgatc ctgaaacgca
    39721 agtcgttctt tctcggttga agagtgactt tgcgcaagta atcgatgatt tattagagaa
    39781 tcgtacagta gagttaaagt ggcagcaaga aggatacaat gtaggcgttg tggttgcagc
    39841 tgccggttac cccgaagaat atgagacagg aatggttcta ccagattttc tggaagaaga
    39901 gctttctgtt tactatgctg gagtttcctc aaataacggc aaactagttg gagcaggagg
    39961 acgtctttat ttagtagaag catacggtga gacgatcaaa gaagcacaga aaaaaattta
    40021 caccgcgtta atggataaaa cgacagatgg aacattttat cgtacagata ttggagaaaa
    40081 agctgccaat agcgaactga tcgtgaagta aatcagctgc ttccatttat aaaaaataca
    40141 acaaacataa tcggaagcgc cgattttagt gaagcctttt cataaagagt gtggtacact
    40201 tttgttgtgt atataaataa ggaggagttc agatgaaaaa aattcaatta ggtacttctg
    40261 atcagaaagc agctgcagtg atcttaggct gtatgcgtat caacaatgcg gaagatccag
    40321 taaaagtgat tgagacagct tatgaaaacg gcattgattt ttttgaccat gcagatattt
    40381 atggaaacgg tgaatgtgaa gaaatctttg cagatgctct agcaaaaaca tcaatcaaac
    40441 gagaagatct ttttattcaa acaaaatgtg gaatcgttcc aggtgtgatg tttgattttt
    40501 ctaaaaaaca tatcattgaa tctgtagaaa aaagtttgac ccgtctgaaa atggattatg
    40561 tggatgcttt acttcttcat cgtccagata cattggtaga accagaggaa gtagctgaag
    40621 cgtttgatga attggaacgg tcaggaaaag ttcgttattt tggcgtcagc aatcaaaaac
    40681 caatgcaaat cgaattgctg aaaaaatatg ttcgtcaacc attagtagct aatcagctgc
    40741 aatttggctt gaaacatact ggaatgatcg atcaagggat tcatgtcaat atgacagatg
    40801 aagcaagtat cgatcatgac ggtagtgtac ttgattattc ccgattgcat gatatgacga
    40861 tccaagcatg gtcaccatac cagtatggat tttttgaagg tgtatttatc gggaacgaaa
    40921 aattccctga attaaaccaa acattagaag aactagcagc taaatatcaa acgacaccaa
    40981 ctggcttggc ttctgcttgg attttacgcc atccagccaa tatgcaagtg atcgccggta
    41041 cgatgaacgt agggcgaatc aaggaaatcg ctcaagcttc agaaattaat atgtctcggg
    41101 aagattggta cgaattgtac cgtgctgctg gaaacatttt accttaaaga ttgtcataag
    41161 caggtcaaaa gggctataaa ttaaaaagac agaataactt gaaagctgtt ttcagcttcg
    41221 agatattctg tcttttttta ttgcaaatgg aaagagtatc attgtacgga tttcgtttat
    41281 tagttatttt tgtttttaag gaaaagctgt gtccgatatt cttttggaga aatcccttgt
    41341 tttttcttaa ataaataact gaaatagtta gggtcattat aaccaatttc aaaagcaata
    41401 tctgaaatac gatagtctga ttgctgcaat aatttttttg ctaattcgat tctttgttct
    41461 gtcagataat cgatgaatgt ttggttaaac gattgagaaa aaatcgtact gaaataagag
    41521 ggacttaatg caacttgttg agcgacccaa gttaaggaca tatctggatc ggtaaaattt
    41581 tctttgataa aatccaatgc atggttcaaa attggctgat atttgttcct tgaaggctgt
    41641 gagggcagag cagttaagaa agaaatgaac atataaactg tcgaacggta aagcgagtcg
    41701 tcggcgctga ttgctaagcg ttgactggtg gatgtgcaat gaaccatttc ttgcgggatt
    41761 ttcttttctt tcaggcagac atgctggagt agcttagtca agacaaataa acgatataat
    41821 cgtgttcgag ttggtgtgcc ttgtggtttc gtcaagtgtt cgatgtactt agatacttcg
    41881 tttttcgata atgaagacaa atcaagtgag aggtcaaagt aaaagacaga atcaactgga
    41941 agtttcgctt cttgaatcaa atcctcatag ctgataattt gttctttaag ggaactggcg
    42001 gtttcaagta tcatctgcgt gatctcaaaa gatgcaggta attcgctgat tctttctgcg
    42061 acaggaccaa tggcgacaga aaaattattt ttttgtccct tttctaattc atggaccaac
    42121 gtttgggcta cctgataact ggtctcgatc acattttctt caagagaatc aactattaaa
    42181 aatttgatga atcgagagga aacagcagag aagatcactt gttgattctc cctaaataaa
    42241 aacttcaaat aatcactgag atgagcgtaa tcttcaaagt ctgtactgac ttggtttgct
    42301 gctaataata cggtcatttt tttccctgct aaagagcgat tgaaagattc agcaagtttc
    42361 aatgtttcac tgatcgtaag tgagcctttg aacaaaccgt tcaagaaatg attttttttc
    42421 aattcgaaaa caagatcaga agaagtgtgc gtttcttttt gctgcaaatc taaagcagtt
    42481 actgcttttt ctattgcttg tttcaattcg ctacttttaa tcggtttaag cagataatca
    42541 tctgcctgta cctggattgc tcctttcata taatcgaaat cgtcgaatcc actaataaat
    42601 atgagacgag tccaaggaag tagtttcctt gcttcttttg aaaaagtcaa cccgtccatg
    42661 aaaggcattt ggatatcagt cagaatgatg tcgggctgta agtcgataat tgaagacagt
    42721 gccatctcac catctccagc ttcgccgaca taagtgattg gataataaga accaaggaca
    42781 gttatttgtt cccgcaagtt attgcgaatg aggtattcgt cttcgatgat aaaaacttta
    42841 tacatgtttg tttcctccaa ttgttttggg agatagaggg atcatcagtg tgacagttgt
    42901 tccttctcgg tatttgctgt caatcgtgat aggtttttga tcatttccgt aataaagaag
    42961 gagacgcttg tttacgttgt aaagtccgta gccgatactg aaatctgaat cgatcccttt
    43021 ggctaactct ttacgtattt ctgccagttt ttctgcaggg atgccaatcc cgttgtctat
    43081 aactttgaat tggatcatct catcaatttt gcatgtggta atacgaataa atcctttgtg
    43141 acggacgtac ttcgttccat ggtatacagc attttcaatc aatggctgca agatcatttt
    43201 taaaatagaa acaggaaaaa gtgtttctgg tatatcgatc ttatagtcaa gcatatggcc
    43261 gtatcttatt tttaagatga ctagataatc attgatatgg ctaatttctt tttcaagagg
    43321 aatccagtct tgtcctttgc ttaaagacgt acgaaaaaag ccggaaagtg catgggtcat
    43381 agtcagagct tcttcaaagc agtcttgttc gatcaaagaa ataatggcat ccaaactgtt
    43441 ataaataaaa tggggagtaa tctgagcttg caatacacga acttcacttt gtgctaaatg
    43501 gtattgtttc aaagcgttct cccgtaaaga ctggtttaga tcttccgcca ttttattcat
    43561 gctagcggta agtgtttgta attccagtgt ttccggcagg gttaaacgaa agccaaggtt
    43621 ccctgaagaa agttgttctg acatctcgat cagctgattg atcggttctt ggacctttct
    43681 ctctaaaaag cgacgattct ttttcgcata gaaaaaaatg gtactaagaa ctccaagttc
    43741 cactattgtt aatagaatga gcgagcggac aagtgtcgta ttttttttgc ttgctaagtc
    43801 gatctccaca cgaacaaaat cttgtaagat atctgacagc agttgagtga cagaatctac
    43861 ctgattgatg atctcttcat tggtatccat aaaatctttt gccaagatat tattgatcag
    43921 ctcgttttga taatcttcca gtgtatcgat cgtacgtagc gatacatcta acgtactaat
    43981 ttctttaagt gtatttgtat tatttttgat ctctcgaata tcattcctaa gttcttctag
    44041 gatattttcc tcattgaact catctacggt tacttggcca gaaatcaagt cccacatact
    44101 ttccaaaact ttttgatcaa ccttcaaagc aattgcatct gctttttgga tatttgcgat
    44161 cgtctgttga taaatcaaaa gctgttgcga atataacagt atgctcagta gcaaagggat
    44221 aacagcaaat agaagcatgg ctttgtttgc ttgggcaaaa atctgtttta tgcttaaatt
    44281 ttttgtcatg atacttctcc agtcaataat ttctggttgg gatgttagct atattctgct
    44341 gggaaaagac agtctcggaa ataaaaatat ctttaggtaa tgtttttcct gtttgaatat
    44401 ttccaaaata acgagtaacg gtattggcta cataccagcc gacatcaggt ttacactcga
    44461 ctacacaatt gacgattcct gagcgtagct tctcgatcat atcttcttgt ccatcaatag
    44521 ttacaatgat caaatcttct ccaggtttga ttgatgtttc ttttatcgca gctaaagcac
    44581 cgtgagtcat ttcatcacta tggctgtata atacatcgat ttcggataaa tcatttgttt
    44641 ctaaatatcg tttcatctgg tcttttcctt tcatccgaat aaaatcaccg acaattcggt
    44701 gaacaatagt catcggaggt ttatggtcga tggttccatc tttttttagg gttggtttac
    44761 gattgattga ttccaaaaat ccttcggagc gtagcttggt cggtgtggag ttctctgaac
    44821 cagcaagttc taggattctg atttcctgtt tctttgaatt tgtgaagtga ttgctgacaa
    44881 ataaacccgc ccgatttcct tctgctttga aactggggcc aatgtgggtg acgaaaagct
    44941 cgggatcatt cgtttgaata tgtctgtcga caagtatgac agggatccct gctcgctttg
    45001 cttcttttaa tacagagtcc cacccggatt cttgcaaagg agtaaaaatg atcatatcca
    45061 ctttataggc aatgaacgtc cgtatatctt ggatttgccg ttcctgattc atgtagccat
    45121 ttcggtagag aacttggtaa tcttcttttt caagcgcagt gataattgat tcagtatgct
    45181 ttttccgcca gctactctcc gttcctgatt gcgaaaaacc aacgactagt gggtcaggtg
    45241 cttcctttgt attaggtgta caccccatca tttggtaagc gattacaaca agggctaaaa
    45301 aaaataaaac acgtttcact ttaaatcact ccatctggat ttgttagtac tatcttatca
    45361 gataaaaggg aaaatcaaag tcaaaaggaa tcaatcgaaa aaaatccaag aaacttaaaa
    45421 aaatccaaga caaaaaaagt acacctaaaa aatttaaagt aaatcccata aaaaaatcaa
    45481 tgttaacgct ttctttatta gctatactcg gtgtatcaat tgaaatgggg gataaaaaat
    45541 gaaaaacaaa tttagaaaga ttgttgggac tatcgctatt ttgaccgcaa gcattctatt
    45601 aagtgcgtgt ggcaattcag gatctgcaga tgatagtacg gggtatgtag gaatcgctat
    45661 gccgaccaaa tcagcagaaa gatggatcgc tgatgggaac aacatggtct cggaattaga
    45721 aaaactgggt tataaaacag atcttcaata tggagaagat aaagtagaaa atcaagtagc
    45781 acaaatagaa aatatgatta caaaaggggt agatacactt gtgattgctt ctatcgatgg
    45841 ttcggcttta actgatgtgt tagctaaagc aaaagaagca gatatcaagg tgatcgccta
    45901 tgatcgttta ctgatgaatt cagaaaatgt cgattattac gcgacttttg ataattttgg
    45961 agtaggtgta tcgcaagcag cttatattga agagcactta ggattgaaag aaggaaaagg
    46021 accattcacg attgaattat ttggtggctc gcctgacgac aacaacgcac taatcaatta
    46081 caacggagtg atgtctgttt tacaaccata tatggacaac ggtcagttgg tcgtgccatc
    46141 tggacagacg agtttcagtc aaatcgctac gcttcgctgg gatggttcta cggcgcaagc
    46201 aaggatggat aatctgttaa gcgcaaatta tactgaccaa acattggatg ctgttttgtc
    46261 gccatatgat ccaataagtt taggaatcat ttcgtcatta aaaggcgtgg ggtatgggag
    46321 tgaaagtaaa cctttacctg tgattactgg acaagatgcg acagttgctg gcgttaaatc
    46381 gatcattgcc ggggaacaaa cacaaacgat ctttaaagac acacgtatct tagcaaaaaa
    46441 tacgattgaa atgatcaaag caatcagtga tggagaagag gttccggtca atgatactga
    46501 aacttatgat aatggggtga aaactgtccc tacgtattta gcaaatacag tatctgtcga
    46561 taaagataat tatcaagcag agctgatcga caccgattat tataaagaat ccgatctgaa
    46621 aaattaagag gaaccaaaca aggaactaaa gagatctaag tcagagagag gataagaatt
    46681 atggcggatt gtattttaaa aatggcagat atcaccaaaa aattttctgg aatccgagcg
    46741 ttagattgcg tgaatctgat gattgaacgt ggggaaatcc atgcattgtg cggagaaaac
    46801 ggagcgggaa aatcaacatt gatgaatgtc ttgagcggct tgtatccata tggctcctat
    46861 gacggggaaa tcttttatga tggaaagctg tgtcaattta aagatttgaa agatagtgaa
    46921 gaaaaaggca tcgtcatcat tcatcaagaa ttagcactaa gtccttatct ttcaataaaa
    46981 gagaatattt ttctcggaaa tgaacaagca gaaagaggcg tgattgattg ggaactgaca
    47041 gaaaagaaaa catatgaact gctgaataaa gtcggtttgt ctatcgaccc gaatcttcta
    47101 gtatcccaga ttggcgtagg gcaacagcaa ctagttgaaa tagctaaagc attatcaaaa
    47161 tccgtccgtc tgttgatttt ggatgagcct accgctgcat tgaatgaaga agaaagcgcg
    47221 aatttgctga gattgatccg agagttgaaa gagcaaggtg taacctccat cattatttca
    47281 cataagctga atgaaatcgt ggatatcgca gatcgaatca ccattttacg ggatggacag
    47341 acgattgaga cactagaaaa tgaaatgatc agcgaagagc atattattcg cggaatggtg
    47401 ggcagagatt tagttgatcg ttatcctgaa agaaaaccgg atattggaga agtttatttt
    47461 aaagtagaag actggacggt ctatcaccct cttgatcacg agcgaatcat aaacgaccac
    47521 atcaacttgc atttgaaaaa aggagagatt gtaggaatag ctggactgat gggagcgggc
    47581 agaactgaat tcgctatgag tctatttgga cattcttatg gaagcaagat atccggacgt
    47641 atattaaagg aaggggcaga gatatcagtt aagaccgttc cttcagccat tgcaaacgga
    47701 ctcgcttatg tctcggagga ccgaaaatct ttaggactga atctgctgat ggatattcgg
    47761 gaaaacgtga cgctgagcag tttggataaa ataagtcaaa aaggagtatt agataaagaa
    47821 aaagaagtaa ctgaggcaga aaatttccgg aaaaagatgc gtataaaaac aaactccatc
    47881 catcaaattg tcagtagtct tagtggtggg aatcaacaaa aagtcgtttt atccaagtgg
    47941 ctgatgacac aacccgatat tctttttttg gatgagccaa cccgtgggat agatgttggg
    48001 gcaaaatatg agatctacac gattatcgaa gaaatggcag caaatggcaa agctgtctgt
    48061 cttatctctt ctgaacttcc tgaaatatta gggatgtgtg atcgaatcta tacgatgaac
    48121 gaaggacgta ttacaggaga agtctcgcga gaagatgcag atcaggaagt cttgatgaaa
    48181 ttaatgacga aagaagaggt gggttaagtt ggaacaggca aaaaatctta cggataaaaa
    48241 agagaaaaaa ctaaatttga aaagtagagc agctgatctt tttggtcgct acagcatggt
    48301 gatcatttta gcgggactac ttattgcgtt tcaattgatg acggatggta ttttctggcg
    48361 tccattgaat ataactaacc ttgttctgca aaatagccat atattagtac tagctgcagg
    48421 gatgttatta gttgtgctct taggtcatgt ggatttgtcg gtagggtcag tgatggcgtt
    48481 tgttggtgca attgcgggca tgatgatggt caatcgccaa atgaacccgt ggatgacagt
    48541 tatcgcatgt cttgcagtag gcgggctgat cggcgcatgg caaggttttt gggtagctta
    48601 tgcagggata ccggcgttta tcgtgacttt agcaggattg ctgatgtttc gtggactgac
    48661 acaagttgtt ctaggaggcc aatctctagc accttttcca gctgttttcc aaaaaatgtc
    48721 tacgggttat ttaccagact taagcgaagg aagcagcctt cacttcttga cattgatttt
    48781 aggaatgatc atcacgatcg tgttagttgt tggtcaatgg cgaacaagaa atcgtcggaa
    48841 aaatcattta tttgaagtgg aatctccaac agctttcttg gtaaaggcgg tatttacagc
    48901 agtcgttgta ctaggaataa cttatatctt tgcctcttat caagggtatc cgattatttt
    48961 gatcattctt ggtttgatcg ttgcagcata tgggtttttg acaaataaaa cagtagcagg
    49021 tcgacagatt tatgcgacgg gcggaaatcg aaaagcggct gaactatcag ggatcaaaac
    49081 aaagcgaatc acgttctggg tattcgtcaa catgggagtc atggcagctt tagctggatt
    49141 agttttagct gctcgattga atgcggcaac acctcaagca ggtacatcga tggaactgga
    49201 tgcaatggcg gcagtatatt ttggtggtgc ttcgacttcc ggcggcattg gaacaatcat
    49261 gggtgcgatt gtcggtggct tggtcatggg tgtattgaac aatggcatgt cgattttagg
    49321 cgtcggggta gactggcagc aggcaatcaa aggattgatc ttgcttttag ccgttgtgct
    49381 agacttgtat aataagaaaa aacgaacaac gtaggagaac gccggttatt cagaaaaaag
    49441 actgtgacaa gacatttgtc acagtctttt tttcgctaaa agcgaacatt cattgtttcg
    49501 tttgtataga ctaaaaataa tatatgtttt tttaccattc gtataaattc tctttttgct
    49561 cttgattttt ttgcttaatt gagttaaatt acatagggcg ctaaaaaaag ccttgattaa
    49621 attaataatg ttcgtgtttt gctggatgtg aacatcagga ggataatgta atgaaagtat
    49681 ttgattacga agatattcaa ttaattccta ataaatgcat cgtaaatagc cgttctgaat
    49741 gtgatacaac ggtcacatta ggtaaacata cattcaaaat gccagtagtg cctgccaaca
    49801 tgcagacgat tattgatgaa acaatcgcag aattcttagc ggaaaatggt tatttctata
    49861 tcatgcatcg gtttgatgaa gcagctcgaa ttccgtttat caaaaaaatg aaaaagcgtg
    49921 gattgattac ctcgatcagt gttggcgtca agaaagagga atattccttt atagagaagt
    49981 tggcagaaga atcgctaaat cctgattata tcactatcga catcgcgcat ggacatgcta
    50041 actcagttat tgatatgatt cagcatatta aaaaatattt gcctgaaact tttgtgattg
    50101 ccggtaatgt tggtacaccg gaagccgtcc gtgaattgga aaatgcgggc gcagatgcaa
    50161 caaaagtcgg gattggtcca ggaaaagtat gtattacaaa aatcaagaca ggctttggga
    50221 ctggaggctg gcaactggca gctcttagat ggtgtgcgaa agcagctcgc aagccgatca
    50281 tcgctgacgg tgggatcaga acacatggcg atattgcaaa atctgtccgt tttggtgcga
    50341 caatggtcat gattggttca ttgtttgccg gtcacgagga atctcctggc gaaacaaaag
    50401 tcgaaaatgg aatcgtgtat aaagaatact ttggttctgc ttcagaattc caaaaaggcg
    50461 aaaaacgaaa tgttgaagga aagaaaatct ggatccaaca caaaggatct ttgaaagata
    50521 cactagtgga aatgcaacaa gaccttcaat catctatttc ttatgctgga ggaagagatc
    50581 tggaagccat tcgaaaagtt gactatgtca tcgtgaaaaa ttcgattttt aatggagaca
    50641 caatctaaaa aataaataaa atattaggat gttgtgaaaa agttgtcctg cattaagtaa
    50701 taagaacagc caaacaaaaa tagttcttta tatgttttgt ttggctggaa ttattatcgc
    50761 agatacaagc ttttgaacat cattattcag aataagggac tgtgataaga tttttgtcac
    50821 agtcccttat gttttgcaaa aaaagttttt cttttttctc gtaaattcgg taaaatcaaa
    50881 agaaaaaata gacaatagaa aagagaagct tatgtgggaa aaagtagaga aaaaacaagg
    50941 accgatttat cgtcaaatta tggaacagat tatgagagaa atcgaaaacg gaaggatcaa
    51001 tcctggaagt caacttgaat cagaacgaaa actagggcaa ctttttggtg tgaatcgttc
    51061 caccgttgtc catgcattgg aagagcttag ggagcttggg gtactaacga gcaaacgtgg
    51121 cagcggatgg tttgttaatc agactgaatg ggggaaattc gctgttccaa gaatcgattg
    51181 gcggcaaatg atctctcctc gttatgagca gacggactgg tatgagcaaa aaataagaga
    51241 agcaaagcag atccaaaaaa catcgtttct tgatttatac gctagcgaga tgccagatga
    51301 gcttttgccg aattttgagt ttccttctta ttctcttgaa gaaatcttag cggaagaaaa
    51361 agaaatggat cttttaggct atactccgtt acgtaacaaa atcaaacggt atttggaaag
    51421 taaatttgaa ttggcatttt cccccaacca gcttcttgtg actggtggcg gtcagcaagc
    51481 aatctttttg attttacaaa cgattctttc agctggagag gggatagctg tagagtcacc
    51541 ttcttttttt taccgattac ctcttttcaa agcttcagga acgcgtttat ttgggatacc
    51601 gatggatgaa gaagggatcg acttagaaaa actgaaagaa tcgatccata aaaataaatt
    51661 gaaagcagtg cttatcaacc cgaattttca aaaccctaca ggaaaagtga tgagtcaaaa
    51721 acgacgagaa aagctagtag aattgtgtcg acagtatcgg cttccaatta ttgaagacga
    51781 tgtatttacg gatctttcat ttctaaacca agaagaagtc cttcctatac gaacgattga
    51841 tccggataat gtgttatacg tcggttcgtt atctagggtt ttaggaaaaa caacgaaaat
    51901 cggttgggtg ataggacctg agacttttat ctcgcaacta gctcaagcgc aagaaatgat
    51961 ggagttttcg atgaatatcc ttacacaaat cacagtggct aatgtattta atgaagcaat
    52021 tgatttgaaa atgcaagatg taagaaaaaa gttgaaagaa aaaagccgac tgataagcgc
    52081 ttggggagat tcacaatcgt ttttcaagct tcattcaatt ttaggtggtt actatgcctg
    52141 gattacttgg gatgggaaaa aactagaccc cgaactagca gaaaaagtag tagatatggg
    52201 tcttggaatt gcaccaagtc tactttttgg gcaagatata aatggaatca gaatcaatta
    52261 cagtaggctg gatgaaaacc aaataccgtt gttttattca aagatgaaaa ttctagaaaa
    52321 gtggttaaca gaataatttc acaaagtaaa tagattaaag aatataacag cttggattta
    52381 aatttcaagc tgtttctttt ttttcggaaa aagaatacga tataaagaaa ctaagaaaaa
    52441 accattattt gtacgtccgt tcacgatgtg taaataaaat aaatgtttgc aacaaaactg
    52501 tataacacca acgtttttaa atagaaacag atggttgtaa gtttgattag aaaatagaaa
    52561 aaatgcagat gaaatataaa acgctttcct tattgtgatt ttattcacta atgctgttaa
    52621 tatgcggatt ggcataattt cctttcatcg tgaaaatttg tgcttttagc ttgtatttct
    52681 cataaatcca tgataattta ttaatgaaga cggttaaata gtgatacagt tttattcact
    52741 gtttcaatcg cagaatgaag agattttctt atagatgaga gaggaatgtg tgacaaatgg
    52801 caaacaaaaa agcaccgatc gacttccaag cactgctaga tgaagtcaat gcagatttcc
    52861 cagtatatca agcacttgat caagatggaa acgtagttaa caaagattta gtacctgatt
    52921 tatccgatga tgaactcgtt gaattaatga ctcatatggt atggtctcgt gtattagacc
    52981 aacgttcaac agcgctgaac cgccaaggac gcctaggctt ttttgcacca actgctggac
    53041 aagaggcaag tcagctggct agtgcttacg catttgataa agaagatgtt cttcttccag
    53101 gttatcggga tgtacctcaa ctagtaaaac atggtttacc tttatctcaa gcttttttat
    53161 ggtctcgagg acatgctgca ggtaattctt atccggaaga attgaaagct ctaccaccac
    53221 aaattatcat cggtgcacaa tatgtacaag cagccggtgt tgcattaggt ttgaagaaac
    53281 gaaacaagaa aaatgtcgtc ttcacttata ctggtgatgg cggttcatca caaggtgatt
    53341 tttacgaagc aatcaatttc gctggcgctt accatgcaaa tgccgtattt tatatccaaa
    53401 acaacgggta tgcgatctct acacctcgtg aaaaacaaac tgcagcaaaa acacttgcac
    53461 aaaaagctgt cgcagctggg attcctggta ttcaagtcga tggaatggat ccgcttgcag
    53521 tatatactgt atctaaaatg gcacgggaat gggcgatcag tggaaatgga ccagtcctaa
    53581 tcgaaacact gacttatcgt tatggaccac atacactatc tggtgacgat ccaacccgct
    53641 accgtacaaa agacacagat gacgattggc agaaaaaaga tccattgatc cgcttccgta
    53701 aatatttgac ggaaaaagga ctttggtctg aagaaaaaga agaacaagtc atcgaagcaa
    53761 ctaaagaaga aatcaaagca gctattgctg aagccgacaa agtaccaaaa caaaaggttt
    53821 cagatttctt gaaaaacatg ttcgaagtat ctccacaatc aatcaaagaa caaattgcaa
    53881 tttatgaagc gaaggagtcg aaataatcat ggcacaaaaa acaatgatcc aagcaatcac
    53941 agatgcctta gcacttgaac tagaaaatga cgagaatgtc gttgtttttg gtgaagatgt
    54001 tgggaaaaac ggaggagttt tccgggcaac tgaaggattg caggaaaagt tcggagaaga
    54061 ccgagtattc gatactcctt tagctgaatc tggaatcgca ggattgagtt tcggtcttgc
    54121 gttagaagga tttcgtccag ttcctgaaat ccaattcttt ggttttattt tcgaagcaat
    54181 ggacgaagta gtagctcaaa tggctcgtac aagatatcga atgagcggca ctagaaatct
    54241 acccatcaca attcgttcgc catttggtgg cggtgttcat acaccagaat tgcattcgga
    54301 taatttagaa ggtttgatcg ctcagtctcc tggtattcga gtagtgattc cttccaaccc
    54361 ttatgatgca aaaggactat tgatttcttc tattagaagt aacgatccgg ttgttttcct
    54421 agaacacatg aaactatatc gctcattccg agaagaagtg ccggatgaag cttacgaagt
    54481 tcctttggat aaagcagcag ttactcgtga gggaacagat gtatctatta tcacatacgg
    54541 agcaatggtg cgtgaagcaa tcaaagcagc agacaatctt gcaaaagaaa atatatctgt
    54601 tgaaatcatt gatcttcgta cagttgcgcc attagatgta gaaacgatca ttcaatccgt
    54661 ggaaaaaact ggtcgcgtag ttgttgtcca agaagcacaa cgacaagcgg gcgttgccgc
    54721 gcaagtagtt tctgagatct cagaacgcgc tatcctttca ttagaagccc caatcggacg
    54781 agtttctgca cctgatacag tcttcccatt tggacaagca gaaaatgtct ggttgccaaa
    54841 tgcaaaagat atcgaagaca aagtaaaaga aatcgcggaa ttttaagaaa agaagggacg
    54901 aaacaaagtc atggcttatc aatttaaatt
//
LOCUS       DACGLE010000010        53722 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0534
            SAMEA3304449-rid9560453.denovo.010, whole genome shotgun sequence.
ACCESSION   DACGLE010000010 DACGLE010000000
VERSION     DACGLE010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304449
            Sequence Read Archive: ERR985064
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 53722)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 53722)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702225.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702225.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 20:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,045
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 27 of 67
            Pseudo Genes (incomplete)         :: 25 of 67
            Pseudo Genes (internal stop)      :: 19 of 67
            Pseudo Genes (multiple problems)  :: 4 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..53722
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304449-rid9560453.denovo.010"
                     /strain="VREN0534"
                     /db_xref="taxon:1352"
     gene            160..840
                     /locus_tag="IXT20_000707"
     CDS             160..840
                     /locus_tag="IXT20_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HAQ4859178.1"
                     /translation="MKKNMIGTLLLSTLLLGGLATPAFAEGQATSKGDITFTEPTNTV
                     EPLNPTDPSKPVEPADPENPATGQTGPLTLDVVPELPFGTHEIESGTKTYQVDASKND
                     TPYLQVSDRRGVGADGQAQGWNVTVSVSDFVNGSQVLQGAELDFGTSTVKSTSDNEST
                     GPTSQTVTGLSKASAATPIFTAAKDQGLGTWLSVYDPANITLKVPKAAAGTFTADLTW
                     NLVAGPVA"
     gene            930..1964
                     /locus_tag="IXT20_000708"
     CDS             930..1964
                     /locus_tag="IXT20_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HAQ4859179.1"
                     /translation="MNQSKRLFVSFFSILSLFFIFPSISQAKDSAGDFGIKPVFPENQ
                     IDKAIGYFDLLVAPEQNQTLEVIISNSSDEERTFEVSVNPAVTSDGGTIDYSQKNPTL
                     DETLPFDVRDVLLIAKKEINVSAHAETTVPIEVKIPAKSFKGRVLAGIHVSPKEEAET
                     ENATEGAQIKNRIAYNLAVVLQESQETIEPDLKLLSGDLDEVNAKPTVQLRFQNPQPR
                     IISNLIFTSKIFYENQLYIENTSNAFLVAPNSNFHLNLDLAGDKAKAGDYRAEIIAKS
                     GDSNEWRFTQNFTIKKEKAQKVNENSVFAVQEQSFPWLYAGLVAAVLVLWLILLFLWV
                     HKKKKKGETT"
     gene            1978..2313
                     /locus_tag="IXT20_000709"
     CDS             1978..2313
                     /locus_tag="IXT20_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4859180.1"
                     /translation="MKTSILLGGIIASLLFFSWQEPVQADTKSSGTITFYDLSPIPHP
                     SGSVNGASKQQTQLPIYKESNQHGVFPETNEQKIRFLYLIIGIELWLLAWLLLWRRKE
                     KEDEHVATK"
     gene            2297..4777
                     /locus_tag="IXT20_000710"
     CDS             2297..4777
                     /locus_tag="IXT20_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4859181.1"
                     /translation="MLLQNKFSFFRSFFFTSLFVLSIFIFSPSAEAVGATPPAISYDP
                     YTYENIVPPNGGRSAVNGGKLVQLTGANQLPKLTATYLNDGSNDPWKGGTVRFHWESH
                     VKLTNVLDLVNITAQGFAFVAIINLPEHVSSSDVLKAIDWNNAYLYIAGAPIKVTSGS
                     LLSAGSHTLRFGMGSWDTNTLLTDIIAIITRGEFNVDDIPLQFDVTANVAKMTENGDK
                     YDTSPNKILTKGKLQPSMSKKAVFSVDFYDSNNIIQDRMFNSILAPAAGRFYPKLTGG
                     NLNPGAYAFSASAEINTWNSYISPSDSTGKYNTFADTTEYVDGNNQNLPGSTNSRTLT
                     MGLTEGRFDQLPFSRFDRVVNYFTGKNVTQGAILTHTPASTSGLNQTANIVYSGKDAQ
                     GGLLSPVALKVLEQYKLDGTVLPTNLTADSQWGKLLSSASYQVTANWSATELKKGSIH
                     YRLYSKGTQKLATGWTDQLFQTIQSNNGGVNTATSTLPALASGQYYFDYRLVDDVLTN
                     MYPSFAYKWQSEQAGITTLPQITVANFPSVSADSQLKNLSRQPETGDPLSALSGDTIQ
                     ENVTFTLTKSGDAITDKKITISLPENTTYINGSLKLNGTTIADTGIQQGVSVPADLLS
                     KIGDTIHLTYNYQLNTVDTSLQSVSILTKAAVLSSNITLADGAKLPNPVVQTSAKTIL
                     VPKQELTLVNVPDDFTFGNDLPKPLKTSYYESKGDFSFDVRDTRLPSTSPWQLTGTLT
                     SLFKNDQGQELSGTKLYFNHSGSKQLIQQGQNTLIYESDGTAKGEVLVDFPDTDGLLL
                     EVNSSTNAQPGATYQGMVTWELTAGPTS"
     gene            4833..6392
                     /locus_tag="IXT20_000711"
     CDS             4833..6392
                     /locus_tag="IXT20_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ4859182.1"
                     /translation="MRRKLLLLKSEMLEGVDLELFSLLEKNEQRQIKLFQLLLEEKGE
                     VSVNNVLTRLSVDRATLKEDLFYLQANLENIKQLEIVIQEGNILLIRHGNIAAVDVYY
                     HCYLRYSTKYQILLYLLENGSFDRQKLVMELNISSATLSRRVKELNDSLNEFHLKIKS
                     GRLTGPESQIRYFYFQLFWFGRPYLENKKEYSDLSGNTFLDLLQKEFSFAITEEGRLK
                     FEIWLSIMKKRMRCTKKSKPDYSNLPVGLFQTTQFLLDLHTFLTRYFFQYAFPWNQNE
                     TLVFYLFMISNFTLDTHNSHVQEFLISEKYKDDVVAQLNRTFKQSIEEYFSFYVFPEE
                     ISQKIALTVMQNHAHFAYFRGWMNIFGQEGLKQRLTEVEDKQFLVLCERLVRQTFAIV
                     DFTEVDEENARFELFARYASILRTLIKQLELRLQIAYDLPYERLMADIVKEIIQEQLL
                     NTIKYDLVEYEKEKQYDIILTSQLKEYPSQKSAKVFVFTSNEYNYDFPYLNQFLKECY
                     LEKLNLRMNKT"
     gene            6389..7753
                     /locus_tag="IXT20_000712"
     CDS             6389..7753
                     /locus_tag="IXT20_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAQ4859183.1"
                     /translation="MKIVIIGASHEGITAALNLRKLQPKAEVLLIDEDHKDGLCYVSN
                     GINLYLKGKIRSLAEVAHNMRTLQESGAKLITEWRVTELDPDKHQLILASKEGKNETI
                     TYDKLIVATGSSPVTLYKQIEAENVYTYKNLVQSKQVLAALKEAKEVVIFGAGYIGLE
                     LADALRNKGYIIHLVDYMPNVLSRYFDKDMINSFQNQLQTKQINFYPNEFLIDWKKSE
                     EKVVSVQLLSQTIKADMVIFSAQTRPNTTLLKEKVALYEDETVIVNEYLQTSDPDIYA
                     IGDIVPVSFDKNKRHLFLPLVTRAVHMARAVALTLSGQPTAYDLRQKITAAVIIDYFL
                     GTVGLTEDEAPFLEQNTGSCSGEFDLFPQYDEENKTVNAKLIYHPDTLEIIGGQLISQ
                     EFLLSDLNLLADIVKHKTTIPQLAVEDFGFLSEYTSRFHYLNELAFKVLAKRANSNRV
                     DKRL"
     gene            7924..8994
                     /locus_tag="IXT20_000713"
     CDS             7924..8994
                     /locus_tag="IXT20_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ4859184.1"
                     /translation="MFLALNEIMHSKLRYALVAGVMFLIAYLVFFLTGLAYGLAQDNR
                     TAVDKWEADSIVLSKDANSNLGMSMITKKIAEEVEGGKVAYLAQTPGVVTSKDSTEEG
                     KINVSFFGIDKNQFIMPNLVEGKAFDNDDEAVGDISLKEEYGLAIGDTVKLSGSDKTF
                     KLTGFTDHAKFNVSPVLYTTINAYQEIRFEKEDTSENARINAIVVRGKISNLPEDLEQ
                     IKISKFINELPGYNAQVLTFGFMIGFLIVIAAIVIGIFIYVLTMQKINIFGVMKAQGI
                     TGGFIARSVVAQTFILSFVGILLGLLGTVGTSLVLPDAVPFQSNWLFFGVISLLMLVV
                     AVLGALFSVRTIVKIDPLKAIG"
     gene            9009..9677
                     /locus_tag="IXT20_000714"
     CDS             9009..9677
                     /locus_tag="IXT20_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ4859185.1"
                     /translation="MKAIEFKEVEKSFQDGDQVVKALKETTMSIDKGEFVAIIGPSGS
                     GKSTFLTLAGGLQTPSSGSVFINDQEFSNQKESERVKLRFKEIGFILQASNLVPFLTV
                     KKQLLLVDKIKHEHRQKEANELFEQLGVDKLINKYPEELSGGERQRVAIARALYNDPS
                     IILADEPTASLDSEKAREVVDILAKESKDKNKATIMVTHDTRLIDKCDRIFMMEDGVL
                     KEKE"
     gene            9697..10275
                     /locus_tag="IXT20_000715"
     CDS             9697..10275
                     /locus_tag="IXT20_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAQ4859186.1"
                     /translation="MVHRYNKDTQQKILKEATDLFMSKGYLGTSTREIAQKVGITQPN
                     LYHYFGDKEKLYTAVLENHLKDVGKALREIVQTSETGFQVTLTKMAQFLIETHLVDLF
                     LMLHDLESNLSKETRDHLFFLWKKNYREPFEEIFSANQSVLRNGISKEIAARHFFLVL
                     APYITRSKRPVEEPLNVAQLIDLYLNGVLGEA"
     gene            10676..11587
                     /locus_tag="IXT20_000716"
     CDS             10676..11587
                     /locus_tag="IXT20_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAQ4859187.1"
                     /translation="MKKIIGAFVFAIIIIIICFQIDVFGQKKIVGQTVSSTNSTVTKT
                     KESFDDTEQETLIKIGKNDRTLYTDDSLTTPLATINGGELAEFLSETKNAYQIKTNDG
                     YTGYLALEDGTKVTKNIQTKPEELSDAVIVLDPGHGGNDTGALSNDEQTEEKDITLST
                     AIKVKKALEDAGATVYLTHTTDELVQLGDICDYSEEKKADVFISLHADSTEYANEATG
                     ITTYYYYGQEETLAQTIADSFTDLPLDSRGISTGNYQVLRENLQPSILIEMGYMNNDS
                     DLKELVTDSYQQQIAASLTQALTTYFQ"
     gene            complement(11965..12882)
                     /locus_tag="IXT20_000717"
     CDS             complement(11965..12882)
                     /locus_tag="IXT20_000717"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HAQ4859188.1"
                     /translation="MSNVIIKATNDQLSQMKAYYRSYLLNKQIPYTSFAAKKDGTTIT
                     AYTSGKVMFQGTNAAIEADKWGEAQDKQAKPAKTASSLPKNISQLSVLGSDEVGNGSY
                     FGPLTVCAAYVKRDQLAELRKLGVRDSKELKDSQIIQLADILKKTIPYKLLVLSPEKY
                     NEIQPKYNAVRMKVALHNQAIHLLLKKIEPEKPEAILIDQFTPEANFKKYARLEKNQL
                     QEKLYFVTKGEQYHLAVAAASIISRASFLEELDKASAEAGITLPSGAGTKSDQVAAKL
                     LEKGGMPMLEKYAKLHFANTEKALKLLKK"
     gene            13084..13521
                     /gene="zapA"
                     /locus_tag="IXT20_000718"
     CDS             13084..13521
                     /gene="zapA"
                     /locus_tag="IXT20_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HAQ4859189.1"
                     /translation="MAHEKTRYKAVIANQTYTIIGRETKHHMDIVTKLINEQLAELKQ
                     LSPQMDNEQAAILMAVNALSDQLKKQERILELEEETAELKKKMIKFTELENRVKRIEA
                     IENEAREVLKENGQADHTIKNHVEAQQILNEKRKGQIKQKSSN"
     gene            13579..14127
                     /locus_tag="IXT20_000719"
     CDS             13579..14127
                     /locus_tag="IXT20_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HAQ4859190.1"
                     /translation="MLSLLILFLLLIAFFSGARRGFALQIIFAIGYVLSFIAAQHFYK
                     PLASHLELYIPYPAVTPTSKLAFFDQVFAFHLDEAFYAGTAFLIILLIGWLLTRFVGV
                     FVHGLTYVPILRQVDWIAGGILSLIMAYVTIFLILQLLAFVPLDVVQNQFGEHSFARF
                     IVERTPFLTNKMYELWVTNILN"
     gene            14242..16602
                     /locus_tag="IXT20_000720"
     CDS             14242..16602
                     /locus_tag="IXT20_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HAQ4859191.1"
                     /translation="MNNRILETLEFEKVKQMVRQFVVTAQGKEELAELVPVSEKQTIT
                     NWLQETEDGLKVQRLRGGIPIPKIENIRPHMKRIEIGADLNGVELAQVSRVLSTTSEL
                     NRFIDDLSDSEIEFARLYMWADQLVTIPVLSRRLKEAIDEDGRVTDDASPELKSIRQN
                     IRRSEQAVREQLDGIVRGKNAKYLSDAIITMRNDRYVIPVKQEYRGVFGGVVHDQSAS
                     GQTLFIEPKQVVDLNNRLRQYQIAERNEIQRILSELSAELVPHRQEIIHNTYVIGKMD
                     LMNAKARFGKEVKAIVPGISEDNHVVLKQARHPLIDQEKVVSNDITIGKDYQAIVITG
                     PNTGGKTITLKTLGLLQLMGQAGLPIPAGEESQIGIFEEVFADIGDEQSIEQSLSTFS
                     SHMTNTVDILSKVNEKSLVLFDELGAGTDPQEGAALAIAILDDLGKKSAYVMATTHYP
                     ELKVYGYNRANTINASMEFDVDTLSPTYRLLIGVPGRSNAFEISSRLGLDTEVIDEAK
                     QLMNDESQDLNEMITDLENRRKMAETEYLEMRHFVSEAQELHDDLKEAYSYFFEEREK
                     EMEKAKKKANEVVSEAEEKAEKIIADIRKMQQQIGQGNVKEHQLIDAKTQLANLHQEE
                     TLKKNKVLKKAKEQKTLKPGDEVLVTTYGQRGTLLRKNGNQWQVEIGILKMNVSEDEL
                     TPVAPQKEPTQRVIHAVRSESSSHVPNQLDLRGKRYEEALSEVDQYLDSAILAGYPQV
                     TIVHGKGTGALRKGITDYLKNHRSVKSFEFAPANQGGNGATIVKFK"
     gene            16689..17003
                     /gene="trxA"
                     /locus_tag="IXT20_000721"
     CDS             16689..17003
                     /gene="trxA"
                     /locus_tag="IXT20_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HAQ4859192.1"
                     /translation="MSQVITDKTFNEETDKGLVLIDFWATWCGPCRMQAPILDQLEQE
                     YDEEEFRIAKMDVDENPETPQQFGIMSIPTLMLKKDGQVVEKAVGVHSKEQLRQMIDQ
                     YL"
     gene            17185..18963
                     /gene="uvrC"
                     /locus_tag="IXT20_000722"
     CDS             17185..18963
                     /gene="uvrC"
                     /locus_tag="IXT20_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HAQ4859193.1"
                     /translation="MNERIKNKLALLPDQPGCYLMKDKNGTIIYVGKAKILKNRVRSY
                     FTGSHNTKTERLVSEIEDFEYIVTESNIEALLLEINLIKKNDPKYNIMLKDDKTYPFL
                     KITNEKYPRLVITRKVLKDKALYFGPYPDVGAANETKKILDRLFPLRKCKPSQTKEPC
                     LYYHLGQCLCPYYFDVDPKVYTKIVDEVKNFLNGGHEKIESEIHQKMEKAAENMEFEK
                     AAEYRDQIRAIETIMTRQKMTNTDLLDRDVFGYAIDKGWMCVQVFFVRQGKLIERDVS
                     MFPFYNEAEEDFLTYLGQFYQENEHFIPKEVLIPDTIDKPSVEALLATKVLQPKRGEK
                     KKLVELASKNASVALNERFDLIARKQERTIGAVERLGNAMNIPTPIRIESFDNSNIMG
                     TNPVAAMVVFIDGKPARKEYRKYKIKTVNGPDDYASMREVIYRRYSRVIREGLPLPDL
                     ILIDGGKGQVDVAKDVLENQLGIDIPIAGMVKNDKHKTNELLFGTDLAVVPLERNSPE
                     FFLLQRIQDEVHRFAITFHRQLRSKNSFASRLDEIEGLGPKRKKMLLKEFKSLKNITA
                     ATTEELQEIGLPKNVAQNVFDKLHQE"
     gene            19108..20007
                     /locus_tag="IXT20_000723"
     CDS             19108..20007
                     /locus_tag="IXT20_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ4859194.1"
                     /translation="MLEVKDLVKTFGNYTAVDHVSFQIPDGKIMGLIGQNGAGKTTTF
                     RLILDFLTQDQGEVLWNGHHLGEKDYDVIGYLPEERGLYPKVTIQDQLLYFAELRGKS
                     RKEIEPKIDFWMEKFQVKGKKTDKVKSLSKGNQQKVQLIATLIHEPKLIILDEPFSGL
                     DPVNAELLKDGILELKKHGSCVIFSSHNMDNVEKICDHLIMLRNGKMVLNGKVHEIRE
                     SFGRTKLFLESGLSQQEVEEIEGVKKVILHEDNTLEITLEDPEAGKEIFTRATQFGYI
                     PMFNQQPPTLEEIFKLKAGEIDA"
     gene            20000..21262
                     /locus_tag="IXT20_000724"
     CDS             20000..21262
                     /locus_tag="IXT20_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ4859195.1"
                     /translation="MRKFWIITKDVYLKNVKSISFLIMILVPFIMLGIIYVAGNFAQQ
                     NSDVNKIGVLADNEQISQQMSQVKTDDFEFKAVDSEEKAQKELSEEKIDAYLVVSTEN
                     DKINGMLYSENSLGQSTQLLIQQQLTGLQSMLRASQLGISPEEVASLSQQASLTRQKV
                     NFDSKGKMTVGEDNSTVQYVVSYVATIVLFIIILTYAQIIAQEIASEKGTRIMEVILS
                     STTAQKHFYGKLTGVLLVALTQMALYGIIFGIGYSQFKDMEMVKNILDGISLDNLIGP
                     FLWYSLLFMIFGILIFSVLAALCGSLVNKAEDTAKAILPVTYLSLGGYMLGLILGASD
                     PNNIVIRITSYIPFLSSYIMPIRLANETVGMGGALISLLVLVAVTFLLMILSANMYKS
                     NVLVYSEGGLWSSLKQSISIMRNERKKA"
     gene            21423..23321
                     /locus_tag="IXT20_000725"
     CDS             21423..23321
                     /locus_tag="IXT20_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphatase class III"
                     /protein_id="HAQ4859196.1"
                     /translation="MDRYLDLLSEKFPSTEDVITEIINLEAILQLPKGTELFLSDIHG
                     EFPAFDHILRIGSGNLKEKIKDLFSEQMTETDISQFTIFTAYPEYALTTDWYKEQDKS
                     QLIHHLIDLLRFTTVKYTRSKVRKGLPKEYSYIIEELLYIDDRINGKKDYVKKIIEQL
                     VLMKEEEKFLTKLAQTIQKSVIDHLHIVGDIFDRGTQAAKVMDRIMDFSSVDIQWGNH
                     DILWIGAYCGSEACLLTLLRIAARYNYLFELEKEYGLNLRPLFSFAHQTYQKNPVFAP
                     KNSKNLSEREQEELEKIHQALSILQFKSEHQLLDRRPEFKMDDRKLLEKIDYEASTID
                     LEGQLYELKGTCFQTIQPDDPKKFLEEEQKVIDSLMYSFQHSLRMQKHMTFLIEKGGM
                     YLTNNQHVLFHGCIPVDEKGQLLAFELDQSYRGKELLGFFEKQITESAKDLTAKEDLA
                     TDLIWYAWSGPFSPLFGKSKMATFERYFLTEPHTHVEKSNPYYHLRDEEWFSEKILAE
                     FDTKEEGSAIINGHTPVKVKKGESPIKANGKVFVIDGGLSKAYQKTTGIAGYSLLCNS
                     YGFQIVTHYPFLPIEQQFAKKSDQTNVKKVIEQQLPRKLNRDTTKGMELQQQITQLKR
                     LLDYRKDD"
     gene            complement(23651..24370)
                     /locus_tag="IXT20_000726"
     CDS             complement(23651..24370)
                     /locus_tag="IXT20_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HAQ4859197.1"
                     /translation="MKPIIGIAGNQLIRATDTFQGNQVSYTPQGFVDAVLDAQGLPLI
                     LPVMSPDSAPQLIGQIDKLILAGGQDVSPQLYMEDPHPKLTETNIQRDQFEQALILEA
                     LKQRKPIFAVCRGLQLLNVVLEGTLYQDLSLYPKWSVKHEQQPTAPQFTTHEVKIVSD
                     SLLSNLLPDPYFVNSYHHQAVKDLSPLLKAIAFSNDGLVEAVQSKDDMHKILGVQWHP
                     ELTHRVHPSEQRLFDFFVNEF"
     gene            24707..25039
                     /locus_tag="IXT20_000727"
     CDS             24707..25039
                     /locus_tag="IXT20_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4859198.1"
                     /translation="MKQVLEALEQIRKAEEENEKRAQTLRQQLKIYATEKEQELETIQ
                     TNYRQQLKQLVEEKEQFEIQELEKEKMKLKAEADKVQATLKDQYQKHQSQAIGAIIER
                     VKETYGRH"
     gene            25026..27029
                     /locus_tag="IXT20_000728"
     CDS             25026..27029
                     /locus_tag="IXT20_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HAQ4859199.1"
                     /translation="MAVTKMEKVTLISDKRNQERILQAVQGIQNVEIRDLYQNRTNNQ
                     WVEKYFPDTTIIDKEAKLSALGNRLKDIQEAIQFIQHHGDSRQKKLHLKRQELSLQDL
                     EANYSEENFINKLKEVLDLKEKWEQLTEERDRLEEEEEWLLHWQQLDIAPSMYASKTT
                     IFEMGTVSAANFESFKEELSKLDDVYMEEIDYTPKQVYLSYVALKKSVAVIEEIAGKY
                     GFNKETYPYEQNPKEKLQETKQQLSIVHEQQKKITTALGQCSGYIQEFEWVEEVTLAV
                     AEREKIKERFIHATYLIVLQGWVDAEEKQNLLHVLNETVSEEDIYVSFETPNSEEIQQ
                     EVPTKLKNHPLVEPFELLTEMYSLPKYEEVDPTPWMTPFYLVFFGMMVADVGYGLLML
                     IGTILAQKLLVLPRGMKRFVKFFEILSIPSIIWGLIYSSFFGQSLPKELFGIRLPFPI
                     LSTTDDVNTILILSVIFGLIQILVGLFVAAKENIRRKDYLSAVSDGFAWQGILIGIVI
                     ALVGAMLIKNTAFVYLGAGLSIIAALCILIVPIIQTPSKVKGAAKGAYNLYGLTGYIG
                     DLVSYTRLMALGISGGSIGAAFNMLVAFMPPAARFSVGILLIIALHALNMFLTLLSAY
                     VHGARLQYVEFFGKFYTGGGRAFDPLKTAEKYVNINHKKKNNK"
     gene            27048..27518
                     /locus_tag="IXT20_000729"
     CDS             27048..27518
                     /locus_tag="IXT20_000729"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HAQ4859200.1"
                     /translation="MMDYLISQNGGMVFAVLAMATATIFSGIGSAKGVGMTGEAAAAL
                     TTSQPEKFGQALILQLLPGTQGLYGFVIAFLIFINLGNDMSVVQGLNFLGASLPIAFT
                     GLFSGIAQGKVAAAGIQILAKKPEHATKGIIFAAMVETYAILGFVISFLLVLNA"
     gene            27539..28123
                     /locus_tag="IXT20_000730"
     CDS             27539..28123
                     /locus_tag="IXT20_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase V"
                     /protein_id="HAQ4859201.1"
                     /translation="MNAIEKIISQMNEAAEQERAALEQEERMKIDQNFEQKRTQVETE
                     HQKQKEKQIELLEKKYRQLRNRQQVEVRQENLNAKQEFLRRLFADAVTEMENWDESEQ
                     IQFIKNALYSLPLTGKVAFIAGEKSAAYLSQTLLDEWNNELPFMMVLSDETVADQAGF
                     LINDQGVQYNFLFSSLVQDIQGTMSFEIANQLFE"
     gene            28138..29124
                     /locus_tag="IXT20_000731"
     CDS             28138..29124
                     /locus_tag="IXT20_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HAQ4859202.1"
                     /translation="MEYHELNPLIRGRELELLSNETFERMIQTNSVEALGEILKSTIY
                     SPYIYDGYEYDFEQNLLKEQSRLFRWLKESAPEPEIVWIYTMRYTFHNLKVLTKAEMT
                     NQNLDHLYIDDGFYSLETLKDAIHTQVSNELPESIMDSIREVRDYFEESTILQGIDVI
                     YDRQFLTEQRRLADKLGYQELLTEVIAFIDLTNITTMARGILQKRTYGFMTTVLSSSG
                     SIEKEVFLNFVDKDMEDYTNFLLETDYADFLRPVIREGNIDLVKLEQLKDDYLSSLYQ
                     EAQTQAFGPLPLLAFLNAKEVESKNLRLLVIGKRSHFESETIRKRVRQVYDA"
     gene            29114..29425
                     /locus_tag="IXT20_000732"
     CDS             29114..29425
                     /locus_tag="IXT20_000732"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HAQ4859203.1"
                     /translation="MTHKIGVVGDKDSVLPFKLFGFDVRYGTTKQTVRQAIEEMAKNE
                     YGVIYVTEQCAALSPETIERYKEQMIPAIVLIPNHQGSLGIGLEEIQKNVEKAVGQNI
                     L"
     gene            29447..31228
                     /locus_tag="IXT20_000733"
     CDS             29447..31228
                     /locus_tag="IXT20_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HAQ4859204.1"
                     /translation="MQIGKIIKVSGPLVMAENMSDASIQDMCLVGDLGVIGEIIEMRG
                     DVASIQVYEETSGIGPGEPVRSTGEALSVELGPGIISQMFDGIQRPLDTFMEITQSNF
                     LGRGVQLPALDHEKKWWFEPTVEAGETVSAGDVIGIVEETKVIKHKIMVPNGIKGTIK
                     TIEKGSFTIDETVCVIETENGDKNLTMLQKWPVRRSRPIKEKLNPDAPMITGQRVIDT
                     FFPVTKGGAAAVPGPFGAGKTVVQHQIAKWADVDMVVYVGCGERGNEMTDVLNEFPEL
                     IDPNTGESLMERTVLIANTSNMPVAAREASIYTGITIAEYFRDMGYDVAIMADSTSRW
                     AEALREMSGRLEEMPGDEGYPAYLGSRLAEYYERSGRVIALGSEGREGSITAISAVSP
                     SGGDISEPVTQNTLRVVKVFWGLDSSLAQKRHFPSIDWIQSYSLYSTEVGKYMDHILQ
                     ADWASMVTEGMRILQEEQQLNEIVRLVGIDSLSDNDRLTLEVAKSIREDYLQQNAFDD
                     VDTFTSREKQFKMLKMILTFGEEGRRALSLGAYLSEIMDGTVSIRERIGRSKYIPETE
                     LEKLDQIIGEIKQTMQTIVSEGGMSND"
     gene            31221..32597
                     /locus_tag="IXT20_000734"
     CDS             31221..32597
                     /locus_tag="IXT20_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HAQ4859205.1"
                     /translation="MIKEYRTIGEVVGPLMAVEKVAGVKYEELIEVRMQNGEIRRGQV
                     LEVQEDKAMVQIFEGTSGINLRDSSVRFLGHPLELGVSEDMIGRVFDGLGRPKDNGPE
                     ILPEKHLDINGEVINPVARDYPDEFIQTGISAIDHLNTLVRGQKLPVFSGSGLPHKEL
                     AAQIARQATVLDSSDDFAVVFAAIGITFEEAEFFMEDFRQTGAIDRSVMFMNLANDPA
                     IERIATPRMALTAAEYLAYEKGMHVLVIMTDMTNYAEALREISAARREVPGRRGYPGY
                     LYTNLATLFERAGRIRGLKGSVTQIPILTMPEDDKTHPIPDLTGYITEGQIILTRELY
                     KSGIQPPIDVLPSLSRLKDKGTGPGKTREDHAATMNQLFAAYAQGKQAKELAVVLGES
                     ALSDVDKIYAKFAERFEKEYVNQGFYTNRSITETLDLGWELLSMLPRTELKRIKDDML
                     DKYLPEGK"
     gene            32604..33239
                     /locus_tag="IXT20_000735"
     CDS             32604..33239
                     /locus_tag="IXT20_000735"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HAQ4859206.1"
                     /translation="MARLNVNPTRMELTRLKKQLTTATRGHKLLKDKQDELMRQFILL
                     IRKNNQLRQEMEESMTEAMSDFVLANASITEPFIEELFVLPAENVDLSIVDKNIMSVK
                     VPVMNFEYDEQLTDTPLEYGYLNSSAELDRSIDRFTTLLPKLLELTEIEKTCQLMASE
                     IEKTRRRVNALEYMTIPQLEETIYYIRMKLEENERAEVTRLIKVKNMGTTD"
     gene            33269..34624
                     /locus_tag="IXT20_000736"
     CDS             33269..34624
                     /locus_tag="IXT20_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk family potassium uptake protein"
                     /protein_id="HAQ4859207.1"
                     /translation="MIKQSKQIKKRLPAVQLLALGFFLLILVGGGLLTLPFFSNSGHG
                     TNFIDALFTATSAVCVTGLTTLNTAEHWNSAGQFLIMLLIEIGGLGFMMVPILCFALI
                     KKKVSLSTRIILKEALNLEEVSGVMKLMLYILKLAFVIQLIGTVALSFVFVPEFGWLK
                     GIWYSLFHAISSFCNAGFDLLGDSLANHQQNVYLIMVVSALIISGGLGFIVWRDLLEY
                     HQKKKMTIHSKIALTVTGTLLLGGFVVFFFTERNGATLVEGNMFQRLANTFFMSVTPR
                     TAGYYSVDYLHMSHAGLILTMFLMYIGGTSGSTAGGLKTTTLGVLLIQMRSMLKGRTR
                     AEAFGRTIRQGAVLRALTLFFVTLTLCVVAIMILSVTETIPKTSGIEYIAFEVFSAFG
                     TVGLTMGLTPDLTAIGKLIIIALMYIGRVGVLTVVFSLMTKAQRTQSKYKYPEESVMI
                     G"
     gene            complement(34816..36210)
                     /locus_tag="IXT20_000737"
     CDS             complement(34816..36210)
                     /locus_tag="IXT20_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HAQ4859208.1"
                     /translation="MPINSYDNYPLTWRPDKQKLKRPYYRSLIQQLEQDILKNKLQKN
                     TQLPSQRELADYLDLNFTTVGKAYKYGIEKGLLYTNIGSGTFISPNVFESITISTNEV
                     TEHIIDFGLVSSFEECNKFVVPFIQSTSNNPNLVGLLNYQDPKGTNFQLTTAANWLKT
                     QGVIPSLESLSIVSGVQNGLAITLSALFSPGDRIATDRYTYANFIELAQLYHLEIVPI
                     DFDEQGMRPDILIQECRKKKIHGVFLMPSCNNPIGFQISHKRREQLAEVIEKEGLWVI
                     EDDIHSFLTTYYQHTIIPPFQNLLPNQTIYLAGMTKFICSGLRVAYLVVPVHVQSKIE
                     QAIFNINVKTSGLDVEVITQILHSNEANRILETKLSLTKRANQLFDSLFKVEKPTNRV
                     PYYRILPVDPEIDPKIIENDFLANGIRLYHSRRFTTRAQPDAFVRISLSSNNLEILAS
                     GLSIVKEKIKKYEL"
     gene            36302..37273
                     /locus_tag="IXT20_000738"
     CDS             36302..37273
                     /locus_tag="IXT20_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HAQ4859209.1"
                     /translation="MKIINFEEVVASFSFEEAIQVMKKCFFDYESGKISQNPRLVEIL
                     PDGKGKNMFAMMPAYLGENRYFGTKVITAFPDNHQYHLPSHLGEIFLFDAQNGLPKAL
                     INANAVTWIRTAAVSALATDYLANPHAETLALIGAGQQALSHLTAISTIRPITTVFVY
                     DILEERRNTFIAKAKKQYPKITFINAISVEDAVRPAEIICTLTSSKEAFLQKEWVRED
                     AHINAVGTFTPDTREISTSLMETSQIYVDDYSAALTESGDLLIAMAEGDLEESCIVGT
                     LGELVSEKKTFYKGESRPTIFDAVGLAIEDLCCAEYIYNKLEKGALK"
     gene            37270..38052
                     /locus_tag="IXT20_000739"
     CDS             37270..38052
                     /locus_tag="IXT20_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HAQ4859210.1"
                     /translation="MNLDYYVVDAFADEVFKGNPAAVYVLEEWLPEGTMQKIAIENNL
                     SETAFTVKKNQEFELRWFTPDREIDLCGHATLATAFVLFNYYKIPDETIKFSTQSGNL
                     FVTRIKDYYYMDFPSIMPKKVPILSEYEEAIGAKIKEAYLARDLFFVLEDEKTVAKLQ
                     PDFTAIKNFELGIGVIVTAESVQKDFVSRTFFPKLTINEDPVCGSAHSNLIPYWAERM
                     NKTKLSAYQMSSRGGDLECELKEDRVIIGGKAVLFSKGTSFI"
     gene            complement(38221..38592)
                     /locus_tag="IXT20_000740"
     CDS             complement(38221..38592)
                     /locus_tag="IXT20_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HAQ4859211.1"
                     /translation="MKMAHTCVRVKDLEASIEFYQKAFGFEESRRRDFPENKFTLVYL
                     TLPGDDYELELTYNYDHGAYDLGDGYGHIAIAADDLEKLHEQHKAAGLEITDLKGLPG
                     TAPSYYFVIDPDGYKIEVIRG"
     gene            38726..39169
                     /locus_tag="IXT20_000741"
     CDS             38726..39169
                     /locus_tag="IXT20_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HAQ4859212.1"
                     /translation="MDKPIFGEKKQFADYQTRYAAYIVIERDQQIAVIEAPNGAFFLP
                     GGEIEGEETKEEAIVRELIEEMGIVAEISSYLGEADEYFYSNYRATYYYNPGYFFVAD
                     SWKRIGEPTEKTNKIWWVSPQEALVKLKRGSHRWAVERWIEKNKN"
     gene            complement(39212..40021)
                     /gene="yidA"
                     /locus_tag="IXT20_000742"
     CDS             complement(39212..40021)
                     /gene="yidA"
                     /locus_tag="IXT20_000742"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HAQ4859213.1"
                     /translation="MSIKLVAIDIDGTLLNEKREVTKEVKEAIAAAVAKGVSIVLCTG
                     RPLPGVQEQLNELNLFQDNDYVITYNGALVQQTKSGKIIARHGLTHEDFLEIEVMARR
                     VGSHLHSIDDKAIYTPNRDISAYTIHEASLVKMPLKYRTPEEMTPDMNIVKMMMIDEP
                     EILDAAIARLPQTFRDKYTTVKSAPYYFEVLNKEASKGAAVANLAQHLGIDQDEIMAI
                     GDNENDLSMIEYAGLGVAMGNAVSLVKEAANVITSSNDEHGVAEAIKKYVL"
     gene            complement(40040..41407)
                     /locus_tag="IXT20_000743"
     CDS             complement(40040..41407)
                     /locus_tag="IXT20_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAQ4859214.1"
                     /translation="MTTPYKYQMLPMEKVFRDPVHNYIHVQHQVILDLINSKEVQRLR
                     RIKQLGTSSFTFHGAEHSRFTHSLGVYEISRRICDIFSRNFSKEKIGNGGWDDQERLV
                     TLCAALLHDVGHGPYSHTFEHIFHTNHEAITVEIITSPETEVYQILNRVEEGFPEKVA
                     SVIQKTYPNPQVVQMISSQIDADRMDYLLRDAYFTGTEYGTFDLTRILRVIRPYEGGI
                     AFSMSGMHAVEDYIVSRYQMYVQVYFHPVSRGMEVILEHLLHRAHELYQEDASSFELH
                     SQLLLPFLDGSFTLEEYLKLDDGVLGTYFTQWSESADPILNDLAKRFLNRKPLKSATF
                     SGNRDSKLVQELTLLVEKVGYNPVYYTAVNSSYDLPYDFYRPEQGRHRTQIEILRNDG
                     TLIELSQVSQLVAALAGQEQGDERFFFPKEMVDPSLRDHYDLFDETYQEFASHIRNGA
                     LIEIN"
     gene            complement(41391..42221)
                     /locus_tag="IXT20_000744"
     CDS             complement(41391..42221)
                     /locus_tag="IXT20_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HAQ4859215.1"
                     /translation="MNPAIRLDQHIYQKNDFSPFALTDILTEYSKEHQQIFLHFWQYD
                     CTVILGMKDVRTPFLSDGIQTLEDAGYAPVIRNSGGLGIVSDEGILNISLIFPQSDTK
                     KITIDDAYEQMLLLTKYAFPKAPIQAFEITHSYCPGTYDLSIHGKKFAGIAQRRIKNG
                     ISVMMYLSVNGNQEKRGQVMKRFYQSALKEDFGTNGYPAVDPATMATLEELLDQPLFV
                     KEVKEAFAKAFEKLYSASDLQDSTDWLKKEIQLEQWNNQIQRMEQRNNIKELHYDNPL
                     "
     gene            42384..42821
                     /locus_tag="IXT20_000745"
     CDS             42384..42821
                     /locus_tag="IXT20_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HAQ4859216.1"
                     /translation="MELSQGTPISIKLRTKVKQQGEVQDFYFDLKGQMVTIGDTLYIR
                     YKEIQEETGDEIPVTIKLVPDGHVQLIRAGEMRMRLKFGYKERLETSYRTPYGMIQIA
                     TFTKELHVSLKDRPTAGKVRIDYDLFMGPEKIGEYYLTLDFTA"
     gene            42897..43517
                     /locus_tag="IXT20_000746"
     CDS             42897..43517
                     /locus_tag="IXT20_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit delta"
                     /protein_id="HAQ4859217.1"
                     /translation="MEISVFEGANKSELSMIEVAHAILEQRGDVMDFSDLANQIQNYL
                     GKSDSEIRDSLAQFYTDLNIDGSFISLGDNRWGLRSWYAIDSIDEEVNHGMDEEDEDT
                     PRRRKRKKVNAFINDDEDAIDYNDDDPEDTDLTEEDDDDLFDDDDDEDEEIAAYNSDL
                     QEIGADDPTDDEEDLPGNIEEDLSIIDEDDDDEDYEEEEFSDSEKE"
     gene            43706..44212
                     /locus_tag="IXT20_000747"
     CDS             43706..44212
                     /locus_tag="IXT20_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HAQ4859218.1"
                     /translation="MIFTGYKKKRQIEKYRHLLNGLIETDYQKMFRITMNYVHDKEEA
                     LDVMQDSFHKALNYFEKEKEIEHFSAWFYRILIRTALDSWRRQKRTPVDLFEIQDIPQ
                     QLPLETSVAELHMILDKIDSPEKEILILYFFEGFRLKEIATILDMNENTVKTKMYRSL
                     NALRQILE"
     gene            44229..45128
                     /locus_tag="IXT20_000748"
     CDS             44229..45128
                     /locus_tag="IXT20_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 and DUF4163 domain-containing protein"
                     /protein_id="HAQ4859219.1"
                     /translation="MYSQKENLKKLKKTYKEQAIPKGLKEEIRQRFIIEEQSFIKAKR
                     RKRRLQGFGVSLAIVMITTSSLLFNSQVRSFAEDLPILGSVIELILGERFTDRSEKID
                     IQVPKINTQNEKENKTIHGLNQKYFREGQADFEKAEKEYGNFETDHYQVLGDYQKIVD
                     DNRFLVIERQITQTAADSHVEKRYDTIDKKNSVQLSLPLLFKDDTYLSVLTKEVKRQM
                     AEQVKEDPSKYYWTEQDIQEGTIEKPTLVTPTRSFYLNKEHQLVLTFSQYEIAPGYMG
                     TPEFVIPKSVTKTILASEDYLDH"
     gene            45215..46099
                     /locus_tag="IXT20_000749"
     CDS             45215..46099
                     /locus_tag="IXT20_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HAQ4859220.1"
                     /translation="MKRNYLVISIIALSVLLFFEITSVASLITEVKGQTHSSEHSSQS
                     FKQSEQSTIKSQSTETANVKEAIQESAEKVQSQSSQSTQDSTKTSRDPAVKSVAISFD
                     DGPGATTTPQLLRILKEKNVHATFFVLGENTAQHPEIVKQTAEAGHEIGNHTYDHQNL
                     ATLSAQSMTEEVTKADTEIKKVTGKTPTFVRPPYGSVTNVGATIIQRPIIEWSVDSED
                     WKTRNPDLILQKIQATVYDGAIILFHDIYPETIRAVPQVIDYLKEQGYRITTVGDLLG
                     HPTAVENYYGRNDHRPVQ"
     gene            complement(46291..46362)
                     /locus_tag="IXT20_000750"
     tRNA            complement(46291..46362)
                     /locus_tag="IXT20_000750"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(46327..46329),aa:Arg,seq:ccg)
     gene            46422..47756
                     /gene="rpoN"
                     /locus_tag="IXT20_000751"
     CDS             46422..47756
                     /gene="rpoN"
                     /locus_tag="IXT20_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HAQ4859221.1"
                     /translation="MKFEQNFSQKQQQTQKLAMTQQLQQSIQVLQYNSEELLAFVENQ
                     AMENPLVEVVEPEWQPDYIKASSSSYEGEETNYLNQIPDTKGSLFDSLIEQVHLNYRD
                     TFLRKIVLYLVEYIDLNGFLTISLEEAGKEIGATPIQMLDALTLIQQLEPAGVGARSL
                     QECLMLQTERDDYAPELAYIVLEECFEELVERKWKEIAQRFSVDLHAVQQIFDYLQTL
                     SPSPGRIFDRSSELYIIPDVRVLVDDDKNVQVISNRKNQPNIRFQESYFKQMSQQADK
                     ETENYLKERKQEFEWLKKTILQRGDTILRVAQVIVSRQKAFFIDKERPIKPLTLKEVA
                     TEIDVHESTVSRAVNGKYLETDFGVFELKKFFTTRIPTNSTEQTEDLSADTAKKKLQE
                     LVDQEDKNKPLSDQKLVELLKKDEIAISRRTVAKYRDLLGIPSSSKRKRYDN"
     gene            47959..48996
                     /locus_tag="IXT20_000752"
     CDS             47959..48996
                     /locus_tag="IXT20_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SorC family transcriptional regulator"
                     /protein_id="HAQ4859222.1"
                     /translation="MLDEIKMIESVAPDIIEVLQERYKILRNIYWMQPIGRRSLSESM
                     GITERVLRTETDLLKNLNLIDTSKSGMTLTTKGEEVYQSLENFMDQLLGTHQTEQQLA
                     EYFGNQRCIVVSGNSEEQTKVADAFGEVLSEALDRLLPEGENIIAVMGGTTMAVVAEQ
                     LSNLENKKRHNLFVPARGGIGEAITVQANSVSARMAAKANGNHRALYVPEQLSLATYN
                     SLLNEPSIQEVLNLISEANCVIHSIGRALHMAARRKMTEKELVMLKQANAVAESFGYF
                     FNEKGEVVYKVPRIGIELQDLEKVPIIMAIAGGKSKAKAIRAYMKNAPKQTWLITDEA
                     AANEILKGVTL"
     gene            49037..50038
                     /gene="gap"
                     /locus_tag="IXT20_000753"
     CDS             49037..50038
                     /gene="gap"
                     /locus_tag="IXT20_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HAQ4859223.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIQDVDGIEVVAINDLTDAKMLAHL
                     LKYDTTQGRFNGTVEVHEGSFNVNGKEVKVLANRNPEELPWGELGVDIVLECTGFFTS
                     KSAAEKHLTAGAKRVVISAPGGNDVPTIVYNTNHETLTGKETVISGASCTTNCLAPMA
                     KTLNDKFGVVEGLMTTIHAYTGDQMTLDGPHPKGDFRRARAAAANIVPNSTGAAKAIG
                     LVIPELNGKLDGAAQRVPVPTGSLTELVTVLEKEVTVDEINAAMKEASNESYGYNTDE
                     IVSSDIVGMTYGSLFDATQTKVMTVGDKQLVKTVAWYDNEMSYTAQLVRTLEYFANL"
     gene            50148..51338
                     /locus_tag="IXT20_000754"
     CDS             50148..51338
                     /locus_tag="IXT20_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005228679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HAQ4859224.1"
                     /translation="MAKKTVKDIDLKDKKVLVRVDFNVPLKDGVITDDTRIKAALPTI
                     NYVLEQGGKAILFSHLGRVKTEEDKEGKSLAPVAKRLGELLGKEVTFVPETRGEQLEE
                     AIRNMNDGDVVVFENTRFEDIDGKKESKNDTELGKYWASLGDVFVNDAFGTAHRAHAS
                     NVGIASTGIPTVAGFLMEKEIKFIGEAVEEPKRPMIAILGGAKVSDKIGVIENLIPKA
                     DKILIGGGMTYTFYEAKGIKIGNSLVEADKVELAKELIEKAGDKLVLPIDNVCAPEFS
                     NDVETQVIEGDIPDGLMALDIGPASVKLFADTLQGAKTVVWNGPMGVFEMSNFAKGTI
                     GVCEAIANLEGATTIIGGGDSAAAAEQLGFADKFTHISTGGGASLELLEGKELPGLAA
                     INDK"
     gene            51442..52197
                     /locus_tag="IXT20_000755"
     CDS             51442..52197
                     /locus_tag="IXT20_000755"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HAQ4859225.1"
                     /translation="MRKPIIAGNWKMNKTASEAKEFAEAVKNKIPSNDKVDSVIGSPA
                     LFLQELMTSAEGTELKISAQNCYWENSGAFTGETSPAALADLGVHYVIIGHSERREYF
                     HETDEDINKKAKAIFANGMTPILCCGESLETYEAGKTAEWIEGQITAGLADLSAEQVS
                     NMVIAYEPIWAIGTGKSADAQIADEICGVVRQTVEKLYGKEVSEAVRIQYGGSVKPEN
                     IAEYMAKENVDGALVGGASLQADSFLSLLDAVK"
     gene            52313..53611
                     /gene="eno"
                     /locus_tag="IXT20_000756"
     CDS             52313..53611
                     /gene="eno"
                     /locus_tag="IXT20_000756"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HAQ4859226.1"
                     /translation="MSIITDVYAREILDSRGNPTIEVEVYTESGAFGRGMVPSGASTG
                     EYEAVELRDGDKARYGGKGVTKAVDNVNNIIAEAIIGYDVRDQMAIDKAMIALDGTPN
                     KGKLGANAILGVSIAVARAAADYLEVPLYHYLGGFNTKVLPTPMMNIINGGSHADNSI
                     DFQEFMIMPVGAPTFKEALRMGAEVFHALASILKARGLATSVGDEGGFAPNLGSNEEG
                     FEVIIEAIEKAGYVPGKDVVLAMDAASSEFYDKEKGVYVLADSGEGEKTTDEMIKFYE
                     ELVSKYPIISIEDGLDENDWDGFKKLTDVLGDKVQLVGDDLFVTNTQKLSEGIEKGIA
                     NSILIKVNQIGTLTETFEAIEMAKEAGYTAVVSHRSGETEDSTISDIAVATNAGQIKT
                     GSLSRTDRIAKYNQLLRIEDQLGEVAEYKGLKSFYNLKNK"
BASE COUNT        17826 a         9356 c        11350 g        15190 t
ORIGIN      
        1 ttaataaaaa aggaagaata ttcatgtaaa cgattttatt gtttttttct cttttataca
       61 aaaagggtca taatttcaag taagcggatg aattcatgag gattttttgc atactgtctc
      121 tactatactt aggtcactaa gaaaaaggag tgatttatta tgaaaaagaa catgattggg
      181 acattattac tttcaacact tcttctaggc ggcttggcaa caccagcttt tgctgagggt
      241 caagcgactt caaaaggaga tattaccttt actgaaccaa caaataccgt ggagcctctt
      301 aaccctactg atccttcaaa accagtagag ccagcagatc cggaaaatcc agcaactggc
      361 cagacaggtc ctttaacact ggatgtagtt cccgaattgc catttggtac acacgagatt
      421 gaaagcggaa caaaaacgta tcaagtagac gcttcaaaaa acgatacacc ttatctccaa
      481 gtatcagatc gtcgaggtgt aggtgcagat ggccaagcac aagggtggaa cgtgacagtt
      541 tcggtttcag attttgtaaa cggttcacaa gttttgcaag gcgctgaatt agattttgga
      601 acatccactg ttaaaagtac gtcagataac gaatccactg gaccaactag tcaaactgta
      661 actgggttga gtaaagcaag tgcagcgaca ccaatattta cagcagcaaa agatcaaggt
      721 ctgggaacat ggctttctgt ttatgatcca gcaaatatca cactgaaagt gccgaaagca
      781 gcagctggaa cattcacggc tgatttgact tggaacctcg tggctggccc agtagcatag
      841 agtatgaaat aaattcaaca aactgcggtg aaaatactat aggtgaagga ttttttaccg
      901 cagttgttgt tgaaaggatg tggtggacaa tgaatcaaag taaacgactg tttgtttcgt
      961 ttttttctat tttaagtcta tttttcattt ttccgtcaat cagccaagca aaggattcag
     1021 caggagactt tggaatcaaa ccggtatttc cagagaacca aatagataaa gcaatcggtt
     1081 attttgattt attagtggca ccggaacaaa atcagacact agaagtaatt atcagcaatt
     1141 cttcagatga agaacgaaca tttgaggtct ctgtcaatcc ggcggtaacg agtgatggtg
     1201 gaacgatcga ttatagtcaa aaaaacccaa cgttagatga gacattgccg tttgatgtcc
     1261 gtgacgtttt gctcattgcg aaaaaggaaa tcaatgtatc agcacatgca gaaacaactg
     1321 tacctataga agtgaaaatc ccagcaaaaa gctttaaggg acgtgtatta gcgggtattc
     1381 atgtttcacc taaagaagaa gctgaaacag aaaatgctac agaaggagca caaatcaaaa
     1441 atcgtattgc ttataatctc gcagtcgttt tacaagaaag tcaagagacg atagaaccgg
     1501 atctgaaact tttatcagga gatttggacg aggtcaacgc aaagccaact gttcagcttc
     1561 gtttccaaaa tcctcagcct aggattatca gcaatttgat atttacgagc aagatctttt
     1621 atgaaaatca gctttatatt gaaaatacgt ctaatgcatt tttagttgca ccaaattcga
     1681 attttcactt gaatcttgat ttagctggtg ataaagcaaa agccggcgat taccgcgcag
     1741 aaatcatagc aaaatcaggc gacagtaatg aatggcgatt tacacaaaac tttacgatca
     1801 aaaaagaaaa agcacaaaaa gtaaatgaaa attccgtttt tgcagtacaa gaacaatctt
     1861 tcccatggct ttatgcagga ttggttgctg cagttctagt gttatggttg attttgctgt
     1921 ttttgtgggt ccacaagaaa aagaaaaaag gagaaacaac atgatgaggt gatttcgttg
     1981 aaaacatcga tcttattggg agggattatt gcaagtttgc tgttcttcag ctggcaagag
     2041 ccagtccaag cagataccaa aagttcaggt acgattacat tttatgatct aagtccaatt
     2101 ccgcatccaa gtggttcagt caatggcgca tcaaaacagc agacacagct accgatttat
     2161 aaagaatcta atcaacatgg cgtattcccg gagacgaatg aacaaaagat ccgttttctt
     2221 tatttgatca ttggtataga attatggctt cttgcgtggt tgttgttatg gagaagaaag
     2281 gaaaaggagg atgaacatgt tgctacaaaa taaattttca ttttttcgct ccttcttttt
     2341 tacttcgctt tttgttttaa gtatatttat cttttcacct tcggcagaag ctgttggagc
     2401 aacgcctcct gccatttctt atgatccata cacgtatgaa aatatcgtac ctccaaatgg
     2461 tggacgtagt gcagtcaatg gcgggaagct tgtccaacta actggggcta accaattgcc
     2521 taaattgact gctacttatt tgaacgatgg ttcaaatgat ccttggaaag gtggcacggt
     2581 ccgttttcat tgggaaagcc atgtgaaact aacaaatgta cttgatttgg tgaatatcac
     2641 tgcacaagga tttgcatttg tggcgatcat caatttgccg gaacatgtat caagcagtga
     2701 tgtattaaaa gcaattgact ggaacaacgc ctacttgtat attgccggtg cccccatcaa
     2761 ggttactagc ggaagtttat tatcagcagg aagccatacc ttgcgttttg ggatggggtc
     2821 gtgggataca aatactctat tgacagatat tattgctatt attacgcgag gcgaattcaa
     2881 cgtggacgat atcccgcttc aatttgatgt tacagctaat gtcgcgaaga tgacagaaaa
     2941 cggagataaa tatgatacga gtccaaacaa aattttgacc aagggtaaat tacaaccttc
     3001 tatgtctaaa aaggccgttt tttctgttga tttttatgat tcgaataata ttatccaaga
     3061 tcgtatgttc aattcgattt tagctccagc tgcaggtaga ttttatccta aattaactgg
     3121 cggaaacttg aatccaggag catatgcatt ttctgcaagt gcagagataa atacgtggaa
     3181 tagttacatc tcaccaagtg attcaacagg aaaatataat acatttgctg ataccacaga
     3241 atacgtggat ggaaataacc agaaccttcc aggctctacc aatagtcgga cattgacgat
     3301 ggggctgact gaaggacgtt ttgatcagtt gccatttagt cgctttgatc gcgtagtcaa
     3361 ctattttaca ggaaaaaacg ttacacaagg agcaatactg actcatactc cagcaagcac
     3421 aagtggattg aatcagacag ccaatatcgt gtacagtggg aaagatgcgc aaggcggatt
     3481 gctttctcct gttgctttaa aggtattgga gcagtacaag ctagacggaa cggttttacc
     3541 aacaaactta acagccgata gtcagtgggg aaagctcctt tcttctgcat cgtaccaagt
     3601 cacggccaat tggtcagcaa ctgaactaaa aaagggttcg atccattaca ggctttattc
     3661 gaaaggaacc caaaaattag cgacaggatg gacagatcaa cttttccaaa cgatccaatc
     3721 aaataacggg ggtgtaaata cagctacttc tacactacct gcattggctt cagggcaata
     3781 ttattttgat tatcgcttgg tggacgatgt acttactaac atgtatccgt cttttgcgta
     3841 caaatggcag tctgaacaag ctggaatcac cactcttcct caaataacag tggctaattt
     3901 tccatctgtg agtgcagata gtcagctaaa aaaccttagc agacaacctg agacaggaga
     3961 tcccctgtct gctctatcag gagatacgat tcaagaaaat gtgacattca cgttaacaaa
     4021 atctggggat gcgataaccg acaaaaaaat cacgatctca cttcctgaaa atactactta
     4081 tatcaatggt tccttaaaat taaatggtac aactatcgca gataccggca ttcaacaagg
     4141 agtttctgtc ccagcagatc tattgagcaa aataggagat accattcatc ttacttacaa
     4201 ctatcaatta aatactgtag atacttcttt acaatccgtc agcatactga caaaggcagc
     4261 tgtactttcc agcaatatta cattagcaga tggtgctaag cttcctaacc cagttgtcca
     4321 aacatctgct aaaacaattc ttgtccccaa acaagaatta acattagtta atgtaccaga
     4381 tgattttaca tttggtaacg atctccccaa accgttgaag acaagttatt atgaatcaaa
     4441 aggcgatttt tcttttgatg tgcgggatac tcggcttcca agcacgtctc cttggcaact
     4501 gacaggtaca ttgacttctt tatttaagaa tgatcaaggt caagaattaa gcggaacaaa
     4561 attatacttc aatcattctg gatcaaagca attgattcag caaggtcaaa acacgttgat
     4621 ttatgagagt gatggaacag ctaaaggaga ggttctcgtt gactttcccg ataccgatgg
     4681 tttactctta gaggtaaata gcagtacgaa tgctcaacct ggcgcaacgt atcaaggcat
     4741 ggtcacatgg gaactgacag ctgggccaac cagctaagtg attagaattt gtatggatct
     4801 gtgataactg tcacagatcc tctttttcaa ggatgagaag gaaacttctg ctgttaaaat
     4861 cagaaatgct tgaaggagtg gacttggaac ttttcagttt gcttgaaaaa aatgaacaac
     4921 ggcaaatcaa acttttccaa cttttattag aggaaaaagg agaggtttcc gttaataacg
     4981 tacttacccg cttatcagtt gatcgggcta ccttgaaaga agatctgttt tatttacaag
     5041 ctaacttgga aaatatcaaa caattggaaa ttgttatcca agaagggaat attttattaa
     5101 tcagacatgg aaatatagca gcagtcgatg tttattatca ttgttacttg cgctattcta
     5161 caaaatatca gatacttctt tatctgttgg aaaatggctc atttgatcgg cagaaacttg
     5221 tcatggaact taatatcagt tcggctactt tgtctcgcag agtgaaggaa ttaaatgatt
     5281 ctttaaatga gtttcatctg aaaataaaaa gcgggcggct gaccggtcca gaaagtcaaa
     5341 ttcgttattt ttatttccaa ttattttggt ttggacgtcc ctatcttgaa aacaaaaaag
     5401 aatacagtga tttatcagga aacacttttt tagatctgct gcaaaaagag tttagttttg
     5461 ctattacaga agaaggacga ttgaaatttg aaatatggct ttccattatg aagaagcgaa
     5521 tgcgctgtac gaaaaaatcc aaaccagatt atagcaatct tcctgttgga ttgtttcaaa
     5581 cgacacaatt tttattggac ctccatacat ttttgacccg ttacttcttt caatatgcct
     5641 ttccttggaa tcaaaatgaa acgttggttt tttatctttt catgatcagt aactttacat
     5701 tagatacaca taactcgcat gtccaggagt ttttgataag cgaaaaatac aaagacgacg
     5761 tagtagctca attgaatcga acgtttaaac aatcgataga agaatatttc tctttttatg
     5821 tttttccaga agagatttcg caaaagattg cgttgacagt gatgcaaaat cacgctcatt
     5881 ttgcctattt tagaggatgg atgaatattt ttggccaaga aggattaaaa cagcggttaa
     5941 ctgaagtaga agataaacaa ttcctggttt tatgtgaaag attggtcaga caaacatttg
     6001 ctattgttga ttttactgaa gtagacgagg aaaatgcccg ctttgaatta tttgctcgct
     6061 atgcatctat tttacggacg ctgatcaagc agttagaatt acgtctgcaa attgcttatg
     6121 atcttcctta tgaaaggcta atggcggata tcgtgaaaga aatcatccag gaacaattgc
     6181 tgaatacgat caaatatgat ttagtcgaat acgagaaaga aaagcagtat gatatcattt
     6241 tgactagcca attgaaagag tacccgtcac agaagtccgc taaagttttt gtattcacta
     6301 gcaacgaata taattatgat ttcccctatt tgaaccaatt tttaaaagaa tgttatttag
     6361 aaaaattgaa tttaaggatg aataaaacat gaaaatcgtg atcattggtg cttcccatga
     6421 aggcatcaca gctgccctaa atctaagaaa acttcaaccg aaagcagaag ttttactgat
     6481 cgatgaagat cataaagatg gactatgcta tgtttcaaat ggaatcaacc tttatttaaa
     6541 aggaaagata cgtagtttag ctgaagttgc ccataacatg cggactcttc aagaaagtgg
     6601 ggcaaaactg atcaccgaat ggagagtaac tgaacttgat ccagacaaac atcagctcat
     6661 cctcgcatcc aaagaaggaa aaaacgagac cataacctat gataaattaa tcgtagcgac
     6721 aggttcctct cctgtcacct tgtataaaca aattgaagct gaaaatgtct acacctataa
     6781 aaatctagtg caatctaagc aagtacttgc cgctttaaaa gaagcaaaag aagtagtgat
     6841 cttcggtgct ggttacattg gattggaatt agcagatgct ttgagaaata aaggatacat
     6901 tattcatcta gttgattata tgccgaatgt tttatcaaga tatttcgata aggatatgat
     6961 caacagtttt caaaatcagc tgcaaactaa gcaaataaat ttttatccaa atgaattctt
     7021 gatcgattgg aaaaaatcag aagagaaagt agtatccgta caacttctgt ctcaaacaat
     7081 aaaggcagat atggtcattt tttctgccca aactcgtcct aatacgacat tgttaaaaga
     7141 aaaagtggct ttgtatgagg atgagactgt gatagtcaat gaatatctgc agacaagtga
     7201 tccggacatc tatgcgattg gagatatcgt cccagtcagt tttgacaaaa ataaacggca
     7261 tctcttttta cctttagtta ctcgagctgt acatatggca agagcagtag cgctaacttt
     7321 gagtggtcag ccaaccgctt acgacctcag acaaaagata acagcagcag tcattataga
     7381 ctacttttta ggaactgtag ggctgacaga agatgaagcg ccattcttgg aacaaaatac
     7441 cggttcgtgt tcaggagaat ttgatctatt tccacaatac gacgaagaaa acaaaacagt
     7501 aaatgcaaag ctgatctacc atccggatac attagaaata attggcggac agctgataag
     7561 ccaagaattc ttattatcag atttgaacct gcttgcggat atcgtgaaac acaaaactac
     7621 gattccacag ctagcagttg aggattttgg atttctgtct gaatacacct ctcgttttca
     7681 ctacctcaat gagttagctt ttaaagtact agctaagaga gcaaatagca atagagtaga
     7741 taaaagactg tgagaaatat cacggtcttt tattgtgtgg ggatataata aatattaatc
     7801 aatcgagaat aaaaaattca tataatgaaa tagcggaaga attttttgag cataatattc
     7861 gccaaaaatt tatcaagtga taaaattgtt gtataaaatg attatttcag gaggaggagt
     7921 taaatgtttt tagcattaaa tgaaattatg cattcaaagt tacgttatgc gttagttgca
     7981 ggggtgatgt ttttgatcgc ctacctggta ttttttttga ctgggttagc ttatggctta
     8041 gctcaagata atcgaacagc agtcgacaag tgggaggcag attcgattgt gctttcgaaa
     8101 gatgcaaatt caaatctagg gatgtccatg attactaaga agatagcaga agaagtcgaa
     8161 ggtgggaaag tggcgtatct tgcccaaaca cctggagtcg tgactagtaa agacagtaca
     8221 gaagaaggaa aaatcaatgt tagcttcttc ggtatcgata aaaatcaatt catcatgccc
     8281 aatcttgttg aaggaaaagc ttttgataac gatgatgagg cagtaggcga tattagtttg
     8341 aaagaagaat atggtctagc cataggagat acagttaagt tatctggtag tgacaagacg
     8401 tttaaactaa ctggctttac ggatcatgcc aaattcaatg tctcaccagt tctctataca
     8461 accatcaatg cttaccaaga aatccgcttc gaaaaagaag atacaagtga aaatgcacgg
     8521 attaacgcaa tagttgtcag aggtaaaatc agtaatttac ctgaagattt agaacagatc
     8581 aaaatttcta aattcatcaa tgaattacct ggatataatg cgcaagtatt gacttttgga
     8641 tttatgatcg gatttttgat cgttattgcg gctattgtga tcgggatttt catttatgta
     8701 ctgacgatgc aaaagattaa tatctttggt gtaatgaaag cacaagggat cacaggagga
     8761 tttattgcgc gttctgtagt ggcacaaaca ttcattttat catttgttgg tattcttctt
     8821 ggtctattag gaacagtggg aacatccctt gttctacctg atgcggttcc attccaaagc
     8881 aattggctgt tttttggcgt cattagcttg ttgatgttag tagtagctgt gttaggcgca
     8941 ctattttctg ttcggacaat cgtaaaaatc gatccgttga aggcaattgg ataagaagga
     9001 ggaagaacat gaaagcaata gaatttaaag aagtagaaaa aagtttccaa gatggcgacc
     9061 aagtagttaa agcattgaaa gaaacaacca tgagtatcga taaaggggaa tttgttgcaa
     9121 tcatcggtcc ttctggttca ggtaaaagta cttttttgac tttggcaggc ggattgcaga
     9181 cgcctagcag tggctctgta tttatcaatg atcaagaatt cagtaatcaa aaagaaagcg
     9241 aacgcgttaa actacgtttt aaagaaattg gctttatttt gcaagcttcc aatctggttc
     9301 cttttctgac agtaaaaaag cagttattat tagtagataa gattaaacat gaacatcgtc
     9361 aaaaagaagc aaacgaatta tttgagcagc ttggcgtgga taagttgatc aacaagtatc
     9421 cagaagaact atctggtgga gaacggcaaa gggtcgcaat cgctcgtgca ttgtacaatg
     9481 atccgtccat tattctagca gatgaaccaa ctgctagttt ggattcagaa aaggcacgag
     9541 aagtagtcga tatcttggca aaagaatcaa aagacaaaaa taaagcaacg atcatggtta
     9601 ctcacgatac acggctgatt gacaaatgcg atagaatatt tatgatggaa gatggtgtat
     9661 tgaaagaaaa agaataacca aaaagcaggt ggaaaaatgg tccatcgata caacaaagat
     9721 acacagcaaa aaattttgaa agaagcgact gacttgttta tgtccaaggg atatctcggc
     9781 acttcaacaa gagaaatcgc acaaaaagtt gggatcaccc agcccaatct ctatcattat
     9841 ttcggtgata aagaaaaact atatacggca gttttagaaa accacttaaa agatgttgga
     9901 aaagctttga gagaaatcgt tcaaacaagc gaaacgggat ttcaagtaac cttgacaaaa
     9961 atggcgcagt ttttgattga gacacatctg gtcgatttgt ttctgatgct tcacgatctt
    10021 gaaagcaatc tttcaaagga aacaagagat catcttttct ttctatggaa gaaaaattat
    10081 cgtgaacctt ttgaagaaat cttttcagca aaccaatctg tgctaagaaa tgggatttca
    10141 aaagaaatcg cagcaaggca ttttttctta gtgttagcac cttacatcac tcggtcgaaa
    10201 agacctgtgg aagaaccgct aaatgtggcg caattgatcg atttatattt gaatggtgta
    10261 ttaggagaag cgtaaagctg ttatataggg agaaaatagt taggtgaaga agcaagatat
    10321 gacgaagatc gttatatctt gcttctttat ataggtagtt tttcataaat acaaatttgt
    10381 tattctctca tgattatcag ggtatatttt tatttattat gtcggtgttc caggaagtgt
    10441 tttaatttaa taacgaagaa attaattgtt tgtagaatga aggaaactat ttattgggta
    10501 gatgaatcac cacgaatagt caaaaggaga tttcaggata tgccaaaaat gaaaaaaatc
    10561 attttagcag taatgttatt ttctacggtt atatctttta cagccccttt tttgcttact
    10621 taagctgatt ttaagttttg gcccttagtc tggatctacg aggtgagagt caagaatgaa
    10681 aaaaataatt ggagcttttg tatttgcaat cattatcatt attatctgtt ttcaaataga
    10741 tgtttttggt caaaaaaaga tagttggaca aacggtcagc agtacgaaca gtacggttac
    10801 aaagacaaag gaatcatttg atgatacaga acaagagaca ttaatcaaaa tcggaaaaaa
    10861 tgaccgtaca ctatatacag atgactcgct aactacccca cttgcaacta taaacggcgg
    10921 agagctggcg gagtttcttt ctgaaacaaa aaatgcttat cagatcaaga caaatgatgg
    10981 ctatactgga tatttagccc tcgaagatgg aaccaaagtc acaaagaata tacaaacaaa
    11041 accggaagag ctatctgatg cagtgattgt attagatcct gggcatggcg gaaatgatac
    11101 tggggcatta agcaatgatg aacaaacaga agaaaaagat ataacattaa gtacagccat
    11161 aaaagtaaaa aaggcgctag aggatgctgg agcaacggtt tatttgaccc atactacaga
    11221 tgaattagta cagcttggag atatatgcga ctacagtgaa gaaaaaaagg cagatgtctt
    11281 tatcagttta cacgcagact ctacggaata tgcgaatgaa gcaactggaa tcacgacata
    11341 ttattactat ggacaagagg aaacattagc acagacaatt gctgactcct tcactgatct
    11401 gcctctggat tcaagaggaa tctccactgg gaactatcaa gttcttcgag aaaatcttca
    11461 accttctatt ttaatcgaga tgggttatat gaataatgat agtgatttaa aggaattagt
    11521 aacagacagt tatcagcagc agattgctgc aagtttgaca caagcattga ccacttattt
    11581 tcagtaataa ataagcccag ccaaacaaaa atagctcctc atatttttcg tttggccggg
    11641 cttattatcg cagatgcaag cttttaaaca ccgtttaagg aagaggtcgt gagcctgccg
    11701 ttcaactctg agtcacaagg agcaatttca aaaaacagct tctcatgttt gatgactatt
    11761 gtgacttgtg accgaggagt tggcatgaga acaccgttta agaaaaaggt tgtgaaatca
    11821 acgttttgtt ctgagtatta gagaaacaaa cggaaaaata gttcctcata ttttgccgtt
    11881 tgttgagcta ataccgagga tcagatgatt gaacaccgtt tattcggaac aagggactgt
    11941 gacatgactt ttgtcacagt tccattattt ttttagtagc ttcaaagctt tttctgtatt
    12001 ggcaaaatga agtttggcat atttttcaag cataggcatg ccgcctttct ccaatagttt
    12061 tgccgctact tggtcagatt tcgttccagc tcctgacgga agggtgattc cagcttcagc
    12121 tgatgctttg tctaactctt ccaaaaagct ggcacgactg atgatagaag cagcagcaac
    12181 agctaaatga tattgttctc ctttagtaac gaagtacagt ttttcttgta attgattttt
    12241 ttctaaacgc gcatattttt taaaatttgc ttccggcgta aactgatcga tcaaaatcgc
    12301 ttctggtttt tctggctcga ttttttttag cagcaaatgg atcgcttgat tgtgcaaagc
    12361 gactttcatc cgaacggcat tgtattttgg ttggatctca ttgtattttt ctggagataa
    12421 gacaagcagt ttatagggga tggttttttt taagatgtca gcaagctgga tgatttggct
    12481 atcttttaac tcttttgaat ctctaacacc aagtttacgt aattctgcaa gctgatcacg
    12541 tttcacataa gcagcacata ctgttagcgg accgaaatag ctgccatttc cgacctcatc
    12601 actacctaaa acggataatt gactgatatt ttttggcaaa gaagaggctg tttttgctgg
    12661 cttggcttgt ttatcctgtg cctcccccca tttatcagct tctattgctg cattcgtacc
    12721 ttgaaacatc acttttccgg atgtataagc ggtaatggtc gtcccgtctt tttttgcagc
    12781 aaagctagta taaggaatct gtttatttaa caaataagag cgatagtatg ctttcatttg
    12841 actaagttgg tcgtttgttg ctttgataat gacgttgctc ataaaattct cctcgttttt
    12901 atacataaaa ctataccgca actcacgact tttcgttttc taagagattg cgagacatag
    12961 cattttttta aagtttatca tataatagtt aagtatgtga aaaatatttg tggagaacgg
    13021 tgaaagaacc aacttcatgg tacactaaat aaaactttca tcaaatagga ggaaatgatt
    13081 cgtatggcgc acgaaaaaac acgctacaaa gcggtgattg ccaaccaaac atatactatt
    13141 atcggacggg aaaccaagca tcatatggat atcgtaacga agctgatcaa tgaacagtta
    13201 gcagaattga aacaattatc accacaaatg gataatgaac aagcagcaat cttgatggct
    13261 gtcaatgcgc tttcggatca attgaagaaa caagaaagaa tattggagct ggaagaagaa
    13321 acagcagaat taaagaaaaa aatgatcaaa tttacagagc ttgaaaatcg tgtgaaacgc
    13381 attgaagcaa tcgagaacga agctcgggaa gtattgaaag aaaatggtca agcagatcat
    13441 accattaaaa atcatgtgga agcacaacag attttaaacg agaaacgaaa aggacaaatt
    13501 aaacaaaaat cttctaatta agatcctttt atcaaaataa gaaaaattta aaaaagttag
    13561 aagagaaagg aacgtagcat gctgagtttg ctgattttat ttttattgct aattgcgttt
    13621 ttttctggag cacgtcgcgg gtttgctttg caaatcatat tcgcgattgg atatgtattg
    13681 tcatttattg ctgcccagca tttttacaaa ccattagcaa gtcatttgga attatacatt
    13741 ccatacccag ctgtcacccc aacatcaaag ttagcatttt ttgatcaggt atttgccttt
    13801 cacctagatg aagcttttta tgcaggaact gcttttctga ttattttgct aattggctgg
    13861 ctgcttaccc gatttgtggg ggtttttgtc catggattga cgtatgtgcc gatcttgaga
    13921 caggtagact ggattgcagg aggcatcctg tcactaatca tggcttacgt tactatattt
    13981 ttgattttgc agttacttgc ctttgttcca ttagatgtgg tccaaaatca attcggcgag
    14041 catagtttcg ctcgttttat cgttgagaga acgccatttc ttaccaacaa gatgtatgag
    14101 ctttgggtaa cgaatatttt gaattaagga aaatagtgct ggaaaatatt tcgttttttg
    14161 tacagagtag tttatttaag aaggctgcgt agcgtatgtc cggcacagct cttttttcat
    14221 agtgaaagat aaggtgataa aatgaataac cgaatccttg aaacattgga atttgaaaaa
    14281 gtcaaacaga tggtcagaca atttgtagtg actgcacaag gaaaagaaga actggcagaa
    14341 ttggtaccag tgagtgagaa acaaacgatc acaaactggc ttcaagaaac tgaggatgga
    14401 ttgaaagtgc aacgtctgcg aggcgggatc cctataccta aaattgaaaa tatccgtccc
    14461 catatgaaac ggattgagat cggagcagac ttgaatggag tggagcttgc acaagtttcc
    14521 agagtattgt ctacgacaag cgaattgaat cgtttcattg atgatctttc tgacagtgaa
    14581 atcgaatttg cacgtttgta tatgtgggca gatcagttag tcacgatccc tgttttgagc
    14641 cgccgtttaa aggaagcaat cgatgaagac ggacgcgtga ctgatgacgc ttctcctgag
    14701 ttaaagagca taagacagaa cattcggcga agtgaacaag ctgttcgtga acagttagat
    14761 gggatcgtcc gtgggaaaaa tgcaaagtat ttaagtgatg cgatcatcac catgcgaaat
    14821 gaccgctacg ttatcccagt caaacaagaa taccgcggtg tatttggcgg tgtagtccac
    14881 gatcaaagtg cttcaggtca gactttattt attgaaccaa aacaagtggt ggatttgaat
    14941 aatcgtttgc gtcaatacca aatcgctgaa cgaaatgaga ttcaacgtat tttaagtgaa
    15001 ctttctgctg aacttgttcc ccatcgtcaa gaaatcatcc acaatactta tgtgattggt
    15061 aaaatggatc tgatgaatgc aaaagcacga tttggtaaag aagtaaaagc catcgtgccg
    15121 ggaatcagtg aagacaatca tgtcgtcttg aaacaagcaa gacatccact gatcgatcag
    15181 gaaaaagtag tttctaatga catcacaatc ggaaaagact atcaagccat cgtcattaca
    15241 ggacctaaca caggtgggaa aacgatcacg ttgaagactt taggtctgtt acaactgatg
    15301 gggcaagcag gattaccgat tcctgcaggt gaagaaagtc aaatcggtat ttttgaggaa
    15361 gtatttgcgg atataggaga cgaacagtcg attgagcaaa gtctatcgac cttttcttct
    15421 catatgacga acactgtaga tattttatca aaagtcaatg aaaaaagtct ggtattattt
    15481 gacgagctcg gtgcaggaac agatccgcaa gaaggtgctg ctctagcaat cgcaatctta
    15541 gatgatcttg gtaaaaagtc tgcttatgtg atggcaacaa cgcattatcc tgaattgaaa
    15601 gtttatggat ataatcgggc aaatacgatt aatgccagca tggagtttga tgtggataca
    15661 ttaagtccaa cgtatcgttt gctgattggc gttcctggac gaagcaatgc gtttgaaatc
    15721 tctagccgat taggtcttga tacagaagtt attgatgaag cgaagcagtt gatgaatgat
    15781 gagagccaag atttaaacga aatgatcaca gatttagaga atcgccggaa aatggcagag
    15841 accgaatatc tggaaatgcg tcattttgtt tctgaggcac aagaacttca cgatgatttg
    15901 aaagaagctt acagctattt ctttgaagaa cgtgaaaaag aaatggaaaa agcaaagaaa
    15961 aaagcaaatg aagtcgtgtc agaggcggaa gaaaaagcag aaaaaatcat tgctgatatt
    16021 cgtaaaatgc aacagcagat tgggcaagga aatgtgaaag agcatcagtt gatcgatgca
    16081 aagacccaat tagcaaatct acatcaagaa gaaactttga agaaaaataa agtattgaag
    16141 aaggcgaaag aacaaaaaac gttgaaaccg ggagacgaag tgttggttac gacttacggc
    16201 caaagaggga cgcttttacg taaaaacggg aatcagtggc aagtagaaat cggtatatta
    16261 aaaatgaatg tgtccgaaga cgagctgacg cctgttgctc ctcaaaaaga gccgacacaa
    16321 cgagttatcc atgctgtacg atctgaatct agtagtcacg tgccgaatca attggacctt
    16381 cgaggaaaac gatacgaaga agcgctaagt gaggtagatc aatatttgga ttctgccatt
    16441 ctagctggtt atcctcaagt gactattgtc cacggaaaag gaacaggcgc acttcgcaag
    16501 ggaattactg attatcttaa aaaccatcgg agcgtcaaaa gctttgagtt tgcaccggca
    16561 aaccaaggcg gaaatggtgc gacaattgtt aaatttaagt aaagcttact aaaggtaagc
    16621 agatggttcg ctatccaagt atagaaatgc tatactagtc atagatttta aaaatggagg
    16681 tctttgatat gtcacaagta attacagata agacatttaa tgaagaaaca gataaaggac
    16741 ttgtattgat tgatttttgg gcaacttggt gtggtccatg tcgtatgcaa gcaccgattt
    16801 tggatcagtt ggaacaagaa tatgatgaag aagaattcag aattgctaaa atggatgtgg
    16861 atgaaaatcc agaaactcct caacaattcg gtatcatgag cattccaaca ttaatgttga
    16921 aaaaagatgg tcaagtagta gaaaaagctg ttggtgttca ttcaaaagaa caactacgtc
    16981 aaatgatcga tcaatattta taaaaactta cagcaaaaat aagctgaggg tgggactgaa
    17041 tccagttccg tcctcggttt ttttgcggtt gagttaaggt tattcaaatt aatatttttt
    17101 gagtataatt acgttgtcag gctggattgt ttttttgaca gacatgctat aataacttta
    17161 attgaatatg aaggggagta ccagatgaac gaacgaatca aaaacaaatt agctttactt
    17221 cctgatcagc ccggctgtta cttgatgaaa gataaaaatg gaacgatcat ttatgtcggc
    17281 aaagccaaaa tattaaaaaa tcgtgtccgc tcatatttta ctggtagcca taatacgaaa
    17341 acggaacggc ttgttagtga aatcgaagac tttgaataca tcgttacaga atcgaatatc
    17401 gaagcattgc tcttagagat caatctgata aagaaaaatg atcctaaata caacattatg
    17461 ttaaaggatg ataaaaccta tcctttcctt aagataacga atgaaaaata tccccgtttg
    17521 gtgattactc gtaaggtact aaaagacaaa gcactgtatt ttggaccata tcccgatgtc
    17581 ggtgcggcga atgagacaaa gaagatcctc gatcgattat ttcctctgag aaaatgcaaa
    17641 cctagtcaga cgaaggagcc ttgtctttac tatcatttgg gacaatgcct gtgtccttat
    17701 tatttcgacg tagatccgaa agtctatacg aaaatcgttg atgaagtgaa aaacttttta
    17761 aacggaggac acgaaaaaat cgagtcagag atccatcaga aaatggaaaa agctgctgaa
    17821 aacatggagt ttgaaaaagc tgcagaatac cgagaccaga tccgtgccat tgagacaatc
    17881 atgactcgcc aaaaaatgac aaatactgat ttactggatc gagatgtatt tggttatgcg
    17941 attgataaag gatggatgtg tgtccaagtg tttttcgtaa gacaaggaaa gctaattgaa
    18001 cgagatgttt caatgtttcc tttctataac gaagcagaag aagatttctt gacttacctc
    18061 ggtcagtttt atcaggaaaa tgagcatttc attcctaaag aagtcttgat tcctgatacg
    18121 atcgacaagc ccagtgtaga agctttgtta gctacgaaag tgctgcaacc aaaacgagga
    18181 gaaaagaaaa aattagttga attggcgagt aagaatgcct cagttgcttt gaatgaacgg
    18241 tttgatctga ttgccagaaa acaggaacgg acgatcggag cagttgaacg tttaggaaac
    18301 gcgatgaata tcccgacccc aatacgtatc gagtcttttg ataactctaa tatcatgggg
    18361 acgaatcctg tggctgcgat ggtcgtattc attgatggaa aaccggcaag aaaagaatat
    18421 cggaaatata aaataaaaac agtcaacgga ccggatgatt atgcatcaat gagagaagtc
    18481 atctatcgca gatattcgcg agtgattcgc gaagggttgc ctttacctga tttgatcttg
    18541 atcgatggag gaaaaggaca agtagatgtg gcaaaagatg tcttagaaaa tcaattagga
    18601 atcgatattc ctattgctgg catggtaaaa aacgataaac ataaaacaaa tgagttgcta
    18661 ttcggtacag atttggctgt agtaccgctt gagcgaaatt caccagagtt tttcttactc
    18721 cagcgaatcc aagatgaagt ccatcgattc gcaatcactt tccatcggca gctacgcagt
    18781 aaaaacagtt ttgcttcccg attagatgaa attgaagggt taggaccaaa acgaaaaaaa
    18841 atgctgctta aagagttcaa atcgttgaaa aatatcacag cagccacgac agaagaatta
    18901 caagaaatcg gactgccgaa aaatgtcgca caaaacgtat ttgataaatt acatcaggaa
    18961 tgaggaaagg gcactttaca aagaaagtaa agtgtcctat cattttatgt acaacaaaaa
    19021 gagcgtttac gttttgttgg taaataataa atcgcttacg tctggtgtct aaggatatat
    19081 gtgatttctt gaagaaggag tgaagaaatg ttagaagtca aggatcttgt taaaactttt
    19141 gggaactaca cagctgtcga tcatgtctct ttccaaatcc ccgatggaaa aatcatgggg
    19201 ctaattggcc aaaacggggc tgggaagaca acgacttttc gtttgatctt ggattttttg
    19261 acacaagacc aaggagaagt gttatggaat ggccatcatc ttggggagaa agattatgat
    19321 gtcatcggtt atcttccgga ggaacgggga ctttatccga aagtaacgat tcaagaccaa
    19381 ctgctttatt ttgcagaact tagagggaaa tcaagaaaag agattgaacc aaaaatcgac
    19441 ttttggatgg aaaaatttca ggtaaaaggg aaaaagacgg ataaagtaaa atctttgtct
    19501 aaaggaaatc agcaaaaagt ccagctgatc gccacgttaa tccatgaacc taaattgatc
    19561 attttggatg agccattcag cgggttagat ccggtaaatg ctgaattgct aaaagatgga
    19621 atattggaat tgaaaaaaca tggttcatgt gtcatttttt ctagccacaa tatggataat
    19681 gtagaaaaaa tctgcgatca tttgatcatg ttgcgaaacg gaaaaatggt tttgaatgga
    19741 aaagtacatg agattcgtga atcatttggc cgtacgaagt tattcttgga atctggttta
    19801 agccaacagg aagtagaaga aatcgaaggc gtgaaaaaag tgatccttca tgaagacaat
    19861 acgttagaga ttaccttaga agatcctgaa gctgggaaag aaatctttac tcgtgcgact
    19921 cagttcggct acattcctat gtttaaccag caaccgccaa ctttggaaga gattttcaaa
    19981 ttgaaagcag gtgaaatcga tgcgtaagtt ttggattatt accaaagatg tctatttaaa
    20041 aaatgttaag tcgatttctt tcttgatcat gatattagtc ccttttatca tgctgggtat
    20101 catttatgta gcaggaaact ttgctcaaca aaatagtgat gtcaataaaa tcggcgtatt
    20161 ggctgataat gagcaaatca gtcagcaaat gagccaagtc aaaacagatg attttgagtt
    20221 caaagcagtt gattctgaag aaaaagcaca aaaagagctt tcggaagaaa aaatcgatgc
    20281 gtatttagta gtctcaactg agaatgataa gataaatggg atgctttata gcgaaaattc
    20341 attaggacaa agtacacaac tactgataca acaacaactg actggcttgc aatcgatgct
    20401 tcgagcgagt caattaggca tttcaccaga agaagtagct agcttgagcc aacaagcaag
    20461 tttgactcga caaaaagtca attttgattc aaaaggaaaa atgacagtcg gagaagataa
    20521 ctcgacagtc caatacgtcg taagttatgt agcaacgatt gtcttgttca ttattatttt
    20581 gacgtatgcg cagatcattg cacaagagat cgcttctgaa aaaggcacgc ggatcatgga
    20641 agtgatcctg tccagtacga ctgcacaaaa gcatttttac ggaaaattaa caggtgtttt
    20701 acttgttgca ttaacgcaaa tggcattata cggtattatt tttggtatag gatacagtca
    20761 attcaaagat atggaaatgg tcaaaaatat attagacggt atctcattgg ataacttgat
    20821 tggaccattc ttgtggtatt ctcttctatt catgatcttt ggcattttga ttttttctgt
    20881 tttagcagct ttatgtggct ctttggtcaa caaagcagaa gatacagcaa aagcaatttt
    20941 gccagtgact tatctatctc taggcggata catgcttgga ttgatactag gagcttcaga
    21001 tccaaacaat atcgttattc ggatcacttc gtatattcca ttcttatctt catatattat
    21061 gccgattcgc ttagcaaatg agacagtcgg tatgggtgga gcattgattt ccttgttggt
    21121 acttgtagcc gtaacattct tattgatgat cttgtctgca aacatgtaca aatcgaatgt
    21181 actcgtttat agcgaaggcg gattatggag ttcattgaaa caatcgattt caattatgag
    21241 gaatgaacga aaaaaagcat aaaacaatcc taaaatacag aaggaagctg gacatctatt
    21301 gttcccagct tctttttgta tagacctatt ttatttattt tactatattg gatatatatt
    21361 cattgaaaat agtgtataac aactttataa tattgagtat accaattttt ggaggcgttt
    21421 taatggaccg ttatttagat ttactttcag aaaaattccc ctcaacggag gatgtcatca
    21481 cggaaatcat caatctcgaa gcaattttgc agcttccaaa aggaacagag ttatttttga
    21541 gtgatataca tggcgaattt cctgcttttg atcatattct gcgtatcggt tcaggaaatc
    21601 ttaaagaaaa gatcaaagac cttttttctg aacaaatgac ggaaacggat atcagccaat
    21661 tcactatttt tacggcttat ccagaatatg cattaacgac tgattggtac aaagaacaag
    21721 ataaatcgca gctgatccat catttgatcg atcttttacg gtttacgaca gtcaaatata
    21781 cccggtcaaa agttcgaaaa ggattgccga aagaatatag ctatatcatt gaggaactct
    21841 tgtatatcga tgaccgtatc aatggaaaaa aagactatgt aaaaaaaatc attgaacaac
    21901 ttgttttgat gaaggaagaa gaaaaatttt taacgaaact tgcacagacg atccaaaaat
    21961 ctgtcatcga tcatttgcat atcgttggag atatttttga ccgaggaact caagcagcca
    22021 aagtgatgga tcgaatcatg gatttttctt ctgtagatat ccaatgggga aatcatgata
    22081 ttttgtggat tggtgcgtat tgcggctctg aggcatgttt actgacactt ttacggattg
    22141 cagcacgcta taattattta tttgaattag agaaagaata tgggttgaat ttacgtccat
    22201 tgttttcttt tgctcatcaa acttatcaga agaatccagt atttgcgcct aaaaacagta
    22261 aaaatctatc cgaaagagag caagaagaat tagaaaaaat ccatcaggca ctttctatcc
    22321 ttcaattcaa atcagaacat cagttattgg atcgtcgacc tgaatttaaa atggacgatc
    22381 gtaaattgct ggaaaaaatc gactatgaag catcaacgat tgatttagaa ggtcagctct
    22441 atgagttgaa aggaacttgt tttcaaacga tccagccaga tgatccaaaa aaattcttgg
    22501 aagaggaaca aaaagtgatt gatagtttga tgtattcatt ccagcactct ttgagaatgc
    22561 aaaaacatat gacgtttttg atcgaaaaag ggggaatgta tctcacgaac aatcagcatg
    22621 tgctgtttca tggctgtata cctgtagacg agaagggaca gctcttagca tttgaattag
    22681 accaatctta tcgcggaaaa gaactgctgg gcttttttga aaaacagata actgaaagcg
    22741 caaaagatct gactgcaaaa gaagatttag caaccgattt gatttggtat gcgtggagtg
    22801 ggccattttc gcctttattc ggcaagagta aaatggcgac ttttgaacgt tattttctca
    22861 cagagcctca tacgcacgtc gagaaaagca atccttatta tcacttgcga gatgaagagt
    22921 ggttcagtga aaaaatttta gcagaattcg acacaaaaga agaagggtca gcaatcatta
    22981 atgggcacac gccagtaaaa gtaaaaaaag gggagtcacc gatcaaagca aatggaaaag
    23041 tatttgtcat tgacggaggt ttatccaaag cttatcaaaa aacgacagga attgccggtt
    23101 attcactatt atgcaattct tatggtttcc aaatcgtgac acattatccg tttttgccta
    23161 ttgaacaaca atttgcaaaa aaatccgacc aaacaaatgt gaaaaaagta atcgaacagc
    23221 aactaccacg caaactcaat cgtgatacaa caaaaggtat ggaactgcag cagcagatta
    23281 cccagctcaa aaggctgttg gactatcgaa aggacgattg agaagaattc cagtgaacat
    23341 tgttccatag actggagact gagacaaaag tcgtgtcgca gtctcttttt ccggataaac
    23401 ggtgttcttc tgccaactcc tcggtcacaa gtcacactat tcaacaaaca tgaaaagctg
    23461 ttttttgaaa ttgctccttg tgactcagag ttgaacggca ggctcacgac ctcttggtat
    23521 aaacggtgtt caaaagccag tttctcggta ttaagccaaa tttatgcaaa aatgtgaaaa
    23581 gccattttca caaaattttt cttaatactc gaaactaaac agctttttca caaccccctc
    23641 agaaagtcta ttaaaattca ttcacaaaga aatcgaataa gcgctgttca gatggatgta
    23701 cacggtgggt aagttccgga tgccattgaa caccgaggat tttatgcata tcatctttgc
    23761 tttgaaccgc ttcaacgagt ccatcgttag aaaaagcgat ggcttttaat aaaggtgaaa
    23821 gatctttgac tgcctgatga tggtaagaat tcacaaaata tggatcgggg agaaggttgc
    23881 ttaacaaact gtctgaaaca atcttgactt cgtgagtcgt aaattgcggg gcagtgggct
    23941 gttgttcatg ttttactgac cactttggat ataatgaaag atcttgataa agtgtacctt
    24001 ccaaaacgac attcaatagt tggagtcccc ggcagactgc aaaaattggt tttctttgtt
    24061 tcaatgcttc caagatcagt gcctgttcaa attggtctcg ttgaatattt gtttccgtta
    24121 gctttggatg aggatcttcc atgtaaagtt gtggactgac atcttgacca cctgcaagaa
    24181 taagtttgtc gatttgtcca atcagttgag gagctgaatc tggagacatg actggcagaa
    24241 taagaggcaa tccttgagca tctaaaactg catccacgaa tccttgcggt gtatagctca
    24301 cttgatttcc ttggaaagta tctgttgctc gaatcagttg atttccggca atgccgatga
    24361 ttggtttcat ttgtattccc cctaaagtag acttattgta acaaggattt aaaaaaaatt
    24421 aaactatgta agacttgccc ctatacttat ctgttcttgc tgtgttacta tctgtaaaga
    24481 gaaatttcgt gtgaaattca tgacgatagt tgaaaatatt gcttaatttg ttataataaa
    24541 aagtaagcag aaataaaaag gttgggcgta agcttcaagg atctagttct tcaaggggtg
    24601 agaaggacat tttattgaag gggcctgcct atactaagtt atctttagag gtacatactt
    24661 tcaatgaaaa aagaaagcat gtacctcttt tttaaatcga aaggaagtga agcaagtgtt
    24721 agaagctttg gaacaaatcc gaaaagcaga agaagaaaat gaaaagcgtg ctcaaacact
    24781 gcggcagcag ctgaagatct atgcaacaga aaaagagcag gaattggaaa cgatccaaac
    24841 aaactatcga caacaactaa aacagttggt ggaagaaaaa gaacaatttg aaatacaaga
    24901 attagaaaaa gaaaaaatga aattgaaagc agaagcagac aaggtgcaag caactttgaa
    24961 agatcaatat caaaagcatc agagtcaagc gatcggtgcg atcattgaaa gggtgaaaga
    25021 aacttatggc cgtcactaag atggaaaaag tgaccctcat ttcagataaa agaaatcagg
    25081 agaggatttt acaagcagta caaggaatcc aaaacgttga aatccgggat ctgtatcaaa
    25141 atagaacaaa caaccagtgg gtggagaagt attttccaga taccacgatt attgataaag
    25201 aagcaaaatt aagcgcttta ggtaatcggt tgaaagacat ccaagaagct atccaattca
    25261 tacaacacca cggcgatagc cgtcaaaaga aactccattt gaaacgtcaa gaactttcct
    25321 tacaagattt ggaagccaat tattccgaag aaaatttcat aaataaattg aaagaagtcc
    25381 ttgatttaaa agaaaagtgg gaacaactaa ctgaagaacg tgatcgttta gaagaagagg
    25441 aagaatggct tcttcattgg caacagctgg atattgctcc aagtatgtat gcaagtaaga
    25501 cgaccatttt tgagatgggg actgtgagtg cagcaaattt tgaaagtttc aaggaagaac
    25561 tgagtaagct tgatgacgtt tatatggaag aaatagatta tacgccaaaa caagtttatc
    25621 tttcgtatgt tgctttaaaa aaatcagttg ctgttataga agaaattgct gggaaatacg
    25681 ggttcaataa agaaacgtat ccttatgaac aaaatcctaa agaaaaactg caagagacca
    25741 agcagcaatt atccattgtt cacgagcagc aaaagaagat aacgacagcg ttaggtcagt
    25801 gcagtggcta tatccaagaa tttgaatggg tagaagaagt cacacttgcc gttgcagaac
    25861 gagaaaaaat aaaagaacgg tttatccatg cgacttatct gatcgttttg caaggttggg
    25921 tagatgcaga agaaaaacag aatttgctgc atgtgttaaa cgaaaccgta tcagaagaag
    25981 atatatacgt cagtttcgaa acaccaaact cagaagagat tcagcaggaa gtccctacta
    26041 aattgaaaaa ccatccgctt gtcgaacctt ttgaattgct gactgaaatg tacagtctgc
    26101 caaaatatga agaagtagat ccaacacctt ggatgacacc attttatctt gtcttcttcg
    26161 ggatgatggt cgcagatgtt ggttatggtt tgctgatgct cattgggaca atactagctc
    26221 aaaaactact tgttctacca cgcgggatga aacgtttcgt aaaattcttt gagattctct
    26281 cgataccatc gatcatctgg ggattgatct acagttcatt tttcggacag tcattaccaa
    26341 aagaactgtt tggcatccgc ttgcctttcc caatcctatc tacgacggat gacgtcaata
    26401 cgattttgat tttatccgtg attttcggtc tgatacagat tttggttgga ttatttgtgg
    26461 cagcaaagga aaatatcagg agaaaagatt acttgagtgc tgtcagtgat ggatttgcct
    26521 ggcaagggat cctaattggg atcgttattg cgttggttgg tgccatgttg ataaaaaata
    26581 cagcatttgt ttaccttggc gctgggctat cgatcattgc tgccttatgt atcttgatcg
    26641 ttccgattat ccaaacgccg tctaaagtca aaggtgctgc caaaggtgcg tataatcttt
    26701 acggactgac cggctatatc ggtgatttag tcagttatac acgtttgatg gcattgggta
    26761 tttcaggcgg aagtattgga gcggcattca atatgctagt tgctttcatg ccgccagctg
    26821 ctcgattcag tgtagggatc cttttgatca tcgctttaca cgcactgaat atgtttttga
    26881 cactgctaag tgcctatgtc catggcgcac gattgcagta tgtcgaattt tttgggaagt
    26941 tttatacagg aggcggacgt gcctttgatc cgctgaaaac ggcggaaaaa tacgtcaaca
    27001 tcaatcacaa gaaaaaaaac aataaataat tggaggattt ttatataatg atggattact
    27061 taattagtca aaatggtgga atggtatttg cggtattagc gatggcaaca gcgacgattt
    27121 tttcagggat cggttcagct aaaggtgttg gaatgacagg tgaggcagca gcagcattga
    27181 caacaagtca acccgaaaaa ttcgggcagg ctctgattct gcaattgctt ccaggcacgc
    27241 aaggtttgta tggattcgtt attgcctttt tgatttttat caacttagga aatgacatgt
    27301 cagttgtaca aggactgaac tttctaggtg cttctctgcc gattgctttt acaggcttgt
    27361 tttcaggtat tgcccaagga aaagtcgcag cggcaggtat tcaaatttta gcgaaaaaac
    27421 ctgaacatgc tacaaaagga atcatttttg ctgcgatggt tgaaacatac gcgattttag
    27481 gtttcgttat ttccttccta ttagtgttga atgcctaagc ataaaggagg aaaagcagat
    27541 gaacgccatt gaaaaaatca tctctcagat gaacgaagca gcagagcagg aaagagcggc
    27601 gttagaacaa gaagagcgaa tgaagatcga tcaaaacttt gaacaaaaac gtacgcaagt
    27661 tgaaacagaa catcaaaaac aaaaagaaaa acaaatcgaa ttactcgaaa aaaaataccg
    27721 tcagctaagg aatcgccagc aagtagaagt acgacaagaa aatttgaatg cgaagcagga
    27781 atttttaaga cgattgtttg cagatgcggt aactgaaatg gaaaattggg atgaatctga
    27841 acaaatccag tttatcaaaa atgcattgta ttcattacct ttgacaggaa aagtagcatt
    27901 cattgctgga gaaaaatcag cagcctatct ttctcagacg ttgttggatg aatggaacaa
    27961 tgaacttccc tttatgatgg ttttaagtga tgaaactgtt gctgatcaag cgggttttct
    28021 gataaacgat caaggagttc agtataattt tcttttcagc agccttgtac aagatatcca
    28081 aggaacgatg agttttgaga ttgccaatca attattcgaa taaggaggga tcagtgtatg
    28141 gaataccacg aactaaatcc cttgattcgc ggaagagagc ttgaactttt gtccaatgag
    28201 acatttgaac ggatgattca aacaaattct gtcgaagcac ttggtgaaat attgaaatcc
    28261 acgatttaca gtccatatat ttatgacgga tatgaatatg acttcgaaca gaatctttta
    28321 aaggagcaga gccgtctatt tcgctggtta aaggaatcag caccggagcc tgaaatcgtt
    28381 tggatctata cgatgcgtta tactttccat aatctgaagg ttttgacaaa agctgagatg
    28441 acaaatcaaa atctggacca tttgtatatc gacgatggat tttattcatt agaaaccttg
    28501 aaggatgcca ttcatacgca agtttcaaat gaacttcctg aaagcattat ggacagtata
    28561 cgagaagtac gtgattattt tgaagagtct accattcttc agggaattga cgtgatttat
    28621 gatcgacagt ttttgacaga gcagcgacgg cttgccgata agctaggata tcaagaattg
    28681 ctgacagaag tcattgcttt tattgacttg accaatatca caacgatggc tcgaggaata
    28741 ttgcagaaaa gaacatatgg gttcatgacg acggtacttt ccagctcggg aagtatcgaa
    28801 aaagaagtat ttttgaactt tgtagataaa gatatggaag attacacgaa ttttctatta
    28861 gagacagatt atgctgattt tctgcgccct gtcatccgtg agggaaatat tgatctcgta
    28921 aagcttgaac agttgaaaga tgattatcta agttcattgt atcaagaagc acaaacccag
    28981 gctttcggac cactgccact actagccttt ttgaacgcaa aagaggtaga aagcaaaaac
    29041 cttcgtttac tggttatcgg caaaagaagc catttcgaat cagaaacaat cagaaagcgg
    29101 gtgagacaag tctatgacgc ataagatcgg tgtagttggc gacaaagatt ctgttttgcc
    29161 attcaaattg tttggctttg atgttcgtta tggtacaacc aagcaaaccg ttcgtcaggc
    29221 aattgaagaa atggcaaaga atgagtatgg cgtgatttat gtcacagagc aatgtgcagc
    29281 attgagccca gaaacgatcg aacgttacaa ggaacaaatg atacctgcta tcgtattgat
    29341 tcccaatcat caaggatcat tagggatagg gctggaagaa attcaaaaaa acgtagaaaa
    29401 agcagtaggt caaaatatct tatagtatga aaaggagata gaaattttgc aaattggaaa
    29461 aatcataaaa gtctccggac ctcttgtaat ggctgagaac atgtctgatg ccagtatcca
    29521 agacatgtgt ctagtgggag atctaggagt tatcggagaa attatcgaga tgcgtggcga
    29581 tgtggcgtct attcaagtat atgaagagac ttctggaatt ggtccaggcg aaccggttcg
    29641 ctctactggt gaagctttat ctgttgaatt aggaccaggg atcatttcac aaatgtttga
    29701 cggaatccaa cggccattag atacctttat ggaaatcaca caaagtaatt tcttagggcg
    29761 cggagttcaa ttgcccgcat tggaccatga gaaaaaatgg tggttcgaac caacagtaga
    29821 agccggagaa acggtgagtg ctggagatgt tatcgggatc gtagaagaaa caaaagttat
    29881 aaaacataaa atcatggtgc caaatgggat aaaaggaacg atcaaaacaa tcgaaaaagg
    29941 ttcattcact attgatgaaa cagtatgtgt cattgaaacc gaaaatggtg acaaaaattt
    30001 gacgatgctt caaaaatggc cagtaagacg tagtcggcca atcaaagaaa aattaaaccc
    30061 ggatgcaccc atgatcactg gacagcgagt gattgataca ttcttcccag tgacaaaagg
    30121 aggagcagca gctgtacctg gtccttttgg tgcagggaaa acagtcgttc agcatcaaat
    30181 tgcaaaatgg gccgatgtgg acatggttgt ctatgtggga tgtggcgagc gtggaaatga
    30241 gatgacggat gttttaaatg aattccctga attgattgac cctaatacag gagaatcgtt
    30301 gatggaacgt acggtattga ttgctaatac atcaaacatg ccagtagcag ctcgagaagc
    30361 ttctatctat acgggaatca caattgcgga atatttccgt gacatgggtt atgacgttgc
    30421 tatcatggca gactccactt cacgatgggc agaagcactt cgtgagatga gcggacgttt
    30481 ggaagaaatg cctggtgatg aaggttatcc agcttatctt ggttcacggc ttgcggaata
    30541 ctatgaacga tctggaagag tcattgcatt aggatcagaa ggtcgcgaag gaagtataac
    30601 cgcaatcagt gctgtatccc cgtctggagg ggatatttcg gaaccagtca cacaaaatac
    30661 attgcgggtc gtaaaagtat tctggggtct tgattccagt ttagcccaaa aaagacattt
    30721 tccttcgatc gattggatac aaagctattc tctttattca acagaagtag ggaagtatat
    30781 ggatcatatc cttcaagctg actgggcatc catggtcacc gagggcatgc gtattttgca
    30841 ggaagaacag cagctgaatg aaatcgtgcg tttggtaggg attgattccc tttcagataa
    30901 tgaccgtctg acattagaag tggcaaaatc cattcgtgaa gattatttgc agcaaaatgc
    30961 ttttgatgat gtcgatacat tcacttcaag agagaaacaa ttcaaaatgt tgaagatgat
    31021 cttgaccttt ggtgaagaag gacgccgtgc gttgtcgtta ggtgcttatt taagtgagat
    31081 catggacggt accgtatcga ttcgtgaaag gatcggacga agcaaatata tcccggaaac
    31141 agaattagaa aaactagatc aaattattgg tgaaatcaag caaacgatgc aaacgatcgt
    31201 ttcagaagga ggcatgagca atgattaaag aatatcgtac gatcggtgaa gtcgtcggtc
    31261 cattgatggc ggttgaaaaa gttgctggtg tcaaatatga agaattaatc gaagtccgta
    31321 tgcaaaatgg cgagattcgt cgtggacaag tattggaagt ccaagaagat aaagcaatgg
    31381 tccaaatatt cgaaggaaca agtggtatca atttacgtga ctcttctgta cgatttttag
    31441 gccatccttt agaactaggt gtatcggaag acatgatcgg tcgagtattc gatggtttgg
    31501 gacgaccgaa agataacgga ccagagatcc tgcctgaaaa gcatttggat attaacggag
    31561 aagtaatcaa tccagtagct cgggattatc cagatgagtt tatccaaaca ggtatctctg
    31621 caatcgatca tttgaataca ttagttcgtg ggcaaaaatt accagtattt tctggctctg
    31681 gtcttcctca taaagaatta gctgctcaga tcgcgcgaca agcaacggta ctcgattcat
    31741 cagatgattt cgccgtagtc tttgctgcta ttggaatcac ttttgaagaa gctgaatttt
    31801 tcatggaaga ttttcgtcaa acgggcgcaa tcgaccgttc tgtcatgttt atgaatttag
    31861 caaacgaccc tgcaatcgaa cgtatcgcta ctcctcgtat ggctttgact gctgcagaat
    31921 atcttgctta tgaaaaggga atgcatgttt tggttatcat gacagacatg accaactatg
    31981 cagaggcatt gcgagagatt tctgcagcac ggcgagaagt tccaggaaga agaggatatc
    32041 ctgggtatct ttacacgaac ttagctactt tattcgaacg agcaggaaga atccgcggac
    32101 taaaaggatc ggttactcaa attccgattc taacaatgcc agaagacgat aaaactcatc
    32161 caatccctga cttgactggt tatattacag aaggacagat catcttgact agagagcttt
    32221 acaagagtgg gattcagcca ccaattgatg tactcccgtc tctttcgcgg ttaaaagata
    32281 agggaactgg acccggaaag acaagagaag accatgcagc tacgatgaat cagctttttg
    32341 cagcctatgc acaaggaaaa caggcaaaag aattagccgt agttttaggt gaatctgctt
    32401 tatctgatgt agacaagatc tatgcgaagt ttgctgagcg gtttgaaaaa gaatatgtca
    32461 atcaaggatt ctacacgaac cgttcgatta ctgaaacact tgatctaggg tgggaactgc
    32521 tatcgatgtt gccaagaacc gaattaaaac gaatcaaaga cgacatgctg gacaaatatc
    32581 tgccagaagg gaagtgagtt ctgatggcac gattgaatgt gaatccgacc cgcatggaat
    32641 tgactcgatt gaaaaaacag ttgacaactg ccacaagagg gcataaatta ttaaaagata
    32701 agcaagatga actgatgcgt caattcatcc tgttgattcg aaagaacaac cagctgcgtc
    32761 aagaaatgga agagtctatg acagaagcaa tgtctgattt tgtattggca aatgcttcga
    32821 tcacggaacc tttcatcgaa gaattatttg ttttgccggc ggaaaacgtt gatttgtcta
    32881 tcgtagacaa aaatatcatg agtgtaaaag tacctgtgat gaattttgaa tatgatgaac
    32941 agctgacaga cacacctttg gaatacgggt atttgaattc cagtgccgaa ctggatcggt
    33001 caatcgatcg gtttactacc ctgcttccaa aacttttgga actgacagaa atcgaaaaaa
    33061 cttgtcaact gatggcgagt gaaatcgaaa agacacgtcg gcgagtcaat gcattagaat
    33121 atatgacgat ccctcaatta gaagaaacga tctattatat tcgtatgaag ctggaagaaa
    33181 acgagcgcgc agaagtaaca aggttgatca aagtaaaaaa tatgggtacc actgactaag
    33241 tggtatcttt ttttaaaaga ggaaaacgat gattaaacaa agcaaacaaa taaagaaacg
    33301 tttgccggct gttcagcttc tcgcattagg attttttctc ctgattttag ttggcggcgg
    33361 cctacttacc ctaccatttt ttagtaatag tgggcatggg acaaatttta tcgatgcgct
    33421 tttcactgca acttctgctg tctgtgtaac gggattgaca acattgaata cagcagagca
    33481 ttggaacagt gctggtcaat ttttgatcat gcttttgatt gagatcggcg gtcttggttt
    33541 tatgatggtt ccgattctat gctttgcatt gataaagaag aaagtcagtc tcagcacacg
    33601 tattatatta aaagaagcgc tgaatctaga agaagtatca ggtgtgatga agctcatgct
    33661 ttacatccta aaattggcct ttgtgatcca attgattggt acggttgctt tatcttttgt
    33721 atttgtgcct gaatttggct ggttgaaagg tatctggtac agtttgttcc atgcaatctc
    33781 tagtttttgt aatgcagggt ttgatttgtt aggagacagt ttagccaatc accagcaaaa
    33841 tgtttatttg atcatggtgg tatctgcttt gatcatttct ggtgggttag gcttcatcgt
    33901 atggcgtgat ttactggaat atcatcagaa aaagaagatg acgattcatt caaaaatcgc
    33961 attaaccgtt acaggtaccc ttttattagg cggattcgtt gtgttctttt tcacagagcg
    34021 caatggggca acgctagtgg aagggaatat gtttcaacga ttggcaaaca ccttttttat
    34081 gagtgtgaca cctagaacgg ctggatatta ttcagttgat tacctccata tgagccatgc
    34141 aggtctgatt ttaacgatgt ttttgatgta catcggcgga acatctgggt cgacggccgg
    34201 cggattgaaa acaactacgt taggtgtgtt gctgatccag atgcgttcga tgttgaaagg
    34261 acgtacgaga gcggaagcat ttggccgtac catccgacaa ggcgcagtgt tgcgggcact
    34321 tactttattt tttgttacgt tgacgttgtg tgtagtcgcc atcatgattt tatcggtgac
    34381 tgaaacgata cctaaaactt ctggcatcga atatattgct tttgaggtct tctctgcgtt
    34441 tggtacagta ggtcttacga tgggattgac gccagacttg actgcaatag ggaaattgat
    34501 cattatagcg cttatgtaca ttggacgagt tggtgtattg actgtggtct tctctctgat
    34561 gacaaaagca caaagaacac aatcaaaata caaatatcca gaagaaagtg tcatgattgg
    34621 ataatttcat cttctgacag aaacctagtg atttagtaaa ataagaaaat aaaataattt
    34681 ttcttgcttc ttggatactt taagtgcaaa atgttcgttt attttttgag gcactacccg
    34741 ttgttgaacc tcgggtagtg cctctttttt attgaaagga atcaataaga tttgattcct
    34801 ttatttatca ataggttata attcatattt ttttattttt tcttttacga tacttagtcc
    34861 agaagctaaa atttcaaggt tattagagga tagggaaatt cgaacgaatg catctggttg
    34921 cgcacgtgta gtaaagcgtc tggaatgata aagtcggata ccgttagcaa gaaagtcatt
    34981 ctcaattatc ttagggtcaa tctcaggatc aactggaaga attcgatagt aagggacacg
    35041 attcgttggt ttttctactt tgaaaagaga atcaaaaagc tgattggctc tttttgtaag
    35101 acttaattta gtttctaaga tcctatttgc ttcatttgaa tggaggattt gagtgatgac
    35161 ttccacatct aatccagaag ttttcacatt tatattaaaa attgcttgtt ctatcttgct
    35221 ttggacatgt actggaacta caagataagc aactcgcaat ccagaacaaa taaattttgt
    35281 cattccagct aagtagattg tttggttagg taataaattt tggaaaggag gaatgatcgt
    35341 atgttgatag taagttgtta aaaatgaatg gatatcatct tcaataaccc atagaccttc
    35401 tttttcaatc acttcggcta attgctctcg tcttttgtga gagatttgaa accctatagg
    35461 attattacaa gaaggcataa gaaagacacc atgaatcttc ttttttctac attcctgaat
    35521 caatatatca ggtcgcattc cttgctcgtc aaaatcaatt ggaacgatct ctaaatgata
    35581 taactgagcc aattcaatga aattagcgta tgtatatcgg tctgttgcta ttctatcacc
    35641 tggagagaaa agtgcagaaa gtgtgatagc caatccattt tgaacaccgg aaacaataga
    35701 tagagattct aaggacggaa tgactccttg agtttttaac caatttgctg cagtagtcaa
    35761 ttgaaaattt gtgcctttgg gatcttggta attaagcaaa ccaacaagat ttgggttatt
    35821 actagttgat tgaataaacg ggacaacgaa tttattacat tcttcaaaag aactaaccag
    35881 accaaaatca atgatatgtt cggttacttc attggtagag atggtgatgg actcaaagac
    35941 attgggagaa ataaatgtgc cacttccaat attcgtatac agcaaacctt tctcaattcc
    36001 atatttataa gccttgccaa cggtagtaaa atttaaatct aaataatcag ctagctctct
    36061 ttgggagggt aactgagtgt ttttctgtaa tttattcttt aaaatatctt gttctaattg
    36121 ttgaatcaac gatcgataat aaggacgctt caatttttgt ttgtctggtc gccaagttaa
    36181 cgggtaattg tcataagaat taataggcat tttctccact ccttaaaatt gtataccata
    36241 caattttacc attgattgta ttgattttag aagttaatct tttgtgagga gggaattgtc
    36301 tatgaaaatt attaattttg aggaagtagt agcttctttt agttttgaag aagcgattca
    36361 agtaatgaaa aaatgttttt ttgattatga aagtggtaaa atcagccaaa atcctcgatt
    36421 ggtggaaatt ttacctgacg gaaaaggcaa aaatatgttt gcgatgatgc ctgcttattt
    36481 gggggagaac agatattttg gcacaaaagt cattaccgct tttccggata atcatcagta
    36541 tcatttacct tctcatttag gagagatttt cttgtttgat gctcaaaacg gactacccaa
    36601 agcattgata aacgcaaatg cagtcacctg gattcgaaca gctgctgtat ctgcgctggc
    36661 aacagattac ttagcaaatc cgcatgcaga aacacttgct ttaattggag ctggtcaaca
    36721 agcattgtcg catttgactg cgatttctac cattcgtccc atcactacag tgtttgttta
    36781 cgatatttta gaagaaagac gaaatacttt tatcgctaaa gctaaaaaac agtatcctaa
    36841 aataacgttt ataaatgcaa tatcagtaga agatgcagta cgtccagcgg aaatcatttg
    36901 tacgttgacc tctagtaaag aggcatttct tcaaaaagag tgggtaaggg aagacgcaca
    36961 tatcaatgct gtgggcacat ttactcctga tacaagagag ataagtacat cgttgatgga
    37021 aacttctcaa atctatgtag atgattactc agctgcctta acagaaagcg gggacctgct
    37081 tatagccatg gcagaaggag atttagagga atcttgtatt gttggtacat taggcgaatt
    37141 agtttctgag aaaaaaacat tttataaagg agaatcgagg ccgacgattt tcgacgcagt
    37201 tggtctagct attgaagatc tttgttgtgc agaatatatt tataacaaat tagagaaagg
    37261 ggctttaaaa tgaacttaga ttattacgta gttgatgcat ttgcagatga ggtgttcaaa
    37321 ggaaatccag cagcggttta tgtgctagaa gaatggctgc cagaaggtac gatgcaaaaa
    37381 attgccattg aaaacaattt atctgaaaca gcatttacag tgaagaaaaa ccaagaattt
    37441 gagcttagat ggtttacgcc agatcgagaa atcgatcttt gtggtcacgc gacattagca
    37501 acagcatttg ttttatttaa ctattacaaa atacctgatg aaacgattaa attttctact
    37561 caaagcggta atctatttgt gacaagaatt aaagattatt attacatgga ttttcctagt
    37621 attatgccga aaaaagtgcc gatattgtct gaatatgaag aagcgattgg ggctaagatt
    37681 aaagaagcct acttagcacg tgatttattt ttcgtattag aagatgaaaa aacggtggct
    37741 aaattgcagc cagattttac agctatcaaa aattttgaat taggaatagg agtcatcgtg
    37801 acagcagaaa gtgtgcaaaa agactttgtt tctagaacct tctttccaaa attaacaatc
    37861 aatgaagatc cagtatgtgg atcagcacat tctaatctta ttccttattg ggcagaaaga
    37921 atgaataaaa caaaattgtc agcttatcaa atgtcatcaa ggggcgggga tctagaatgt
    37981 gaattaaaag aagatcgtgt gataatagga ggaaaagcag ttttgttttc taaaggtacc
    38041 tcgtttattt gatttctttc aaatgaatta aaaataaatt ggtggagagt ggcaacctta
    38101 tagctacaat ggttgttaaa aaaagacgca aactttttca atttaatgaa aagtttgcgt
    38161 ctcattttaa ttattggtgt ttgtttttta ttcaatcaag agtaaaatgc cccatactaa
    38221 ctatcctcgg atgacttcga ttttataacc atcagggtca atgacaaagt agtaagaagg
    38281 cgcagtgcca ggtaatcctt ttaagtcagt aatctcaaga ccagcagctt tatgttgctc
    38341 gtgtaatttt tctagatcat cagctgctat agcgatatga ccatagccgt ctcctagatc
    38401 atacgcacca tgatcatagt tgtaagtcaa ctctaattca tagtcatccc caggaagtgt
    38461 caaataaaca agtgtaaatt tgttttctgg aaaatcacgg cggcggcttt cttcaaaacc
    38521 aaatgccttt tgataaaatt cgattgatgc ttcaagatct ttcactcgaa cacatgtgtg
    38581 agccattttc atagattcat ctctcctttt ccttttactc aatcataaca aaaagaagta
    38641 aggaaatagt tgatttgtgc ttgttcatga gaaatttctc atgtaaaatg gatagaaaat
    38701 aaaactgtta aggtggaaac tattcatgga taaaccaatt tttggtgaaa aaaagcaatt
    38761 tgctgattat caaacccgtt atgcagcata tattgtcata gaaagagatc agcagattgc
    38821 agtaatcgag gctccaaatg gtgctttttt tcttcctggc ggggaaattg aaggagaaga
    38881 gacgaaagaa gaagcgattg tccgtgagct gatagaagaa atgggaattg tggcagaaat
    38941 ttcttcttat ctgggagaag ctgatgagta tttttactcg aattatcgtg caacttatta
    39001 ttataatcca ggatatttct ttgttgctga ttcttggaag cgtataggag aaccaactga
    39061 aaagaccaat aaaatttggt gggtgagccc tcaagaagcg cttgtgaaat tgaaacgtgg
    39121 tagccaccga tgggcagtcg agagatggat cgaaaaaaat aaaaactgaa agaaagcgag
    39181 tagatcagct ttctctcagt ttttttattt attacagtac atattttttg attgcttcag
    39241 ctacaccgtg ctcgtcgttt gatgaagtga tgacatttgc tgcttctttg acaagagaaa
    39301 cggcatttcc cattgctact cctagaccag catattcaat catggataag tcgttctcgt
    39361 tatctccgat agccatgatt tcatcttgat caataccaag gtgctgtgcg agattcgcaa
    39421 ctgccgcacc tttacttgct tctttattta gtacttcgaa gtaatagggc gcacttttga
    39481 ccgttgtgta tttatcacgg aaagtttgcg gtaaacgcgc aattgctgca tctaaaattt
    39541 ctggttcgtc gatcatcatc attttgacga tgttcatgtc tggtgtcatt tcttcaggtg
    39601 ttcgatattt tagcggcatc ttgactaaac ttgcttcgtg gatcgtataa gcgctgatat
    39661 ctcgattagg cgtgtagatc gctttgtcat cgatcgaatg gagatgactg ccgacacggc
    39721 gtgccattac ttcgatctct aggaaatctt catgtgtcag tccgtgacgg gcgatgattt
    39781 tacctgattt cgtttgttgt acgagtgccc cgttataagt aataacgtaa tcattatctt
    39841 gaaaaaggtt caactcgttt aactgttcct ggacacctgg taagggacgg ccagtgcata
    39901 aaacgatcga aacacctttt gcaacagcag cggcaatcgc ttcttttact tcttttgtta
    39961 cttctctttt ctcatttaat aaagtaccat cgatgtcgat agcgactagt ttgatagaca
    40021 taatttcctc caatatattt taatttattt caatcaatgc gccattacgg atatggctgg
    40081 caaattcttg ataagtttcg tcaaataaat cataatgatc acgcaaggac ggatcaacca
    40141 tttcttttgg aaagaaaaat cgctcatctc cttgttcttg tcccgctaat gcagcaacaa
    40201 gctgactaac ctgtgataat tctatcaaag ttccgtcatt tcgcaaaatc tcgatttgtg
    40261 tgcgatgtct gccttgttct gggcgataaa aatcataagg tagatcgtag ctagaattca
    40321 cagcagtgta ataaacagga ttatatccga ccttttctac taacaaagtg agttcttgga
    40381 ctaattttga atcccggttg ccagaaaaag tagcagattt cagtggttta cgattcaaaa
    40441 aacgtttagc taaatcattt aaaataggat cagcagattc agaccattga gtaaagtaag
    40501 tgcccaagac cccatcatca agtttgaggt attcttctaa agtaaagctt ccgtcaagaa
    40561 atggcagaag taattgagaa tggagttcaa aactgctggc atcttcttga tacaactcgt
    40621 gtgcgcgatg aagcagatgc tccaaaatta cttccatacc acgggaaaca ggatggaagt
    40681 acacttgcac atacatttga tatcggctaa cgatataatc ttccactgca tgcatgccac
    40741 tcatggaaaa agcgatcccg ccttcataag gacgaatcac acgtaatatt cgggtaagat
    40801 caaatgttcc atattctgtt ccagtgaaat aagcatcccg aagcaaatag tccattcgat
    40861 ctgcatcgat ctgactagaa atcatttgga caacttgtgg gttggggtaa gttttttgga
    40921 tcacactagc taccttttca ggaaaacctt cttctactcg gttaagaatc tgatacactt
    40981 ctgtttctgg agaagtgatg atctctacag tgatggcttc atgattcgta tggaagatat
    41041 gttcaaatgt atgagagtaa ggaccatgac cgacatcatg caacagtgcc gcacataaag
    41101 tcaccagccg ttcctgatca tcccagcctc cgttgcctat tttttctttt gaaaaatttc
    41161 gagaaaagat atcgcagatt cgtcttgaaa tctcataaac gcctaatgaa tgagtaaaac
    41221 ggctatgttc tgctccgtga aaagtgaaag aagaagtgcc taattgttta atgcgtcgta
    41281 gacgttggac ttctttcgaa ttgatcaaat ctaagattac ttgatgctgg acatggatat
    41341 agttatggac tggatcacga aaaacttttt ccataggcag catttgatat ttataagggg
    41401 ttgtcataat gtagctcctt tatgttatta cgttgttcca ttctctggat ttggttgttc
    41461 cattgctcca gctggatttc tttttttagc cagtcggtag aatcctgtaa atcacttgca
    41521 gaatacagct tttcaaaagc ttttgcaaat gcttctttga cttcttttac aaaaaggggt
    41581 tggtcaagca gttcttcaag tgttgccatt gtagcaggat ccactgcagg atagccattt
    41641 gtaccaaagt cttcttttaa agcagattga taaaatcgct tcatgacttg cccacgtttt
    41701 tcctgattac cgttgacact taagtacatc ataacagaaa tcccattttt gatccgacgc
    41761 tgagcgatac cggcaaattt tttgccatgg atgcttaaat cataagttcc aggacaataa
    41821 gaatgggtga tttcgaatgc ttgtatcggt gcctttggaa aagcgtattt agtcaatagg
    41881 agcatttgct cgtaagcgtc atctatggtt attttttttg tgtcactttg ggggaaaatc
    41941 aaagaaatat tcaaaattcc ttcatctgat acgatcccta atccacctga atttctgatc
    42001 actggagcgt aacctgcatc ttccagtgtt tggatcccgt cacttagaaa aggagttcga
    42061 acatccttca tacctaagat gactgtgcag tcatattgcc agaaatgtaa aaagatttgc
    42121 tggtgttctt tagaatattc cgttaatata tctgttaatg caaaagggga aaagtcgttt
    42181 ttttgataaa tgtgctgatc tagtcgaatc gcagggttca ttcaaccggc tcctttgtat
    42241 aaattcatat tttctacaat attatactct aaaatcaaag agttgttttg gaaaaaaggt
    42301 tttctgtaaa aatggacaag ttggttgaca aaacaaaaga aactaggaac aatgtagcta
    42361 tgagaagtaa aggagaatga ataatggaat tatcacaagg aacaccgatt tcaatcaaat
    42421 tgcgtacaaa agtaaaacag caaggggaag tgcaagattt ttattttgat ttgaaagggc
    42481 aaatggtgac aattggcgat acgctgtaca tacgttataa agagattcag gaggagacgg
    42541 gcgatgagat tcctgtaacg atcaaattag ttccagatgg tcatgtacag ctgatccgtg
    42601 caggagaaat gcggatgcgt ttgaaatttg gctataaaga acggttagaa acatcctatc
    42661 gaacaccata cggtatgatc cagattgcta cttttacgaa agaactgcat gttagtttga
    42721 aagaccgtcc cacagcaggg aaggttcgaa tcgattatga tctgttcatg ggaccggaaa
    42781 aaatcggcga atattatttg accttggatt ttacagcgta aatataaaag aaaagtttga
    42841 ttttttgtgt ctgattttgt atgataagga gtagactgtg aaaggacgtg tcctgattgg
    42901 aaattagtgt atttgaaggt gcaaacaaaa gcgaattatc aatgatcgaa gtagcacacg
    42961 cgatcttaga acaacgtggc gatgtcatgg atttttctga tttagctaac cagatccaaa
    43021 actatcttgg gaaatcagac agtgaaattc gtgattcact cgcacaattt tatactgact
    43081 tgaatattga cggcagcttt atctcattag gggataatcg ttggggcttg cgttcatggt
    43141 atgctatcga ttcgatcgat gaagaagtaa accatggaat ggacgaagag gatgaagata
    43201 ctccacgtcg ccgtaaacgc aagaaagtca acgcctttat caatgatgat gaagacgcga
    43261 tcgattacaa tgacgatgac ccagaagata cagatctgac agaagaagat gacgatgatc
    43321 tgtttgatga cgatgatgat gaagatgaag aaatcgctgc ttataattct gacttgcaag
    43381 aaatcggagc agatgacccg acagatgatg aagaagactt gccaggaaat atcgaagaag
    43441 atttaagtat catagacgaa gacgatgatg atgaagatta tgaagaggaa gaattttctg
    43501 actctgaaaa agaataaaca ttattttctt acgagaggtt gtgacagaga cgatcagcct
    43561 atcagaataa ggaggtgtga cacgattttt gtcacacctt cttttttgta tataaaaatc
    43621 ccaagtaaat acataaaaaa taaaaaaatt gaaactttta gcgcaccttg tacgtcatat
    43681 taagtgaggc agaaaggagt ggagaatgat ttttactgga tataagaaaa aacggcagat
    43741 cgaaaaatac cgtcacctgc tgaatggact catcgaaact gattatcaaa aaatgttccg
    43801 tatcacgatg aattacgtac atgataaaga agaggcctta gatgtgatgc aagatagttt
    43861 tcataaagcc ctgaactatt ttgaaaaaga aaaagagatc gagcatttta gtgcatggtt
    43921 ttatcggata ttgatccgca cggcattaga tagttggcgc agacaaaaaa ggacgcctgt
    43981 ggacttattt gaaattcaag atatccccca acagttacct ttagaaactt ccgttgcaga
    44041 gctccatatg attttagata aaatagacag tccggaaaaa gagattctga tcctctactt
    44101 ttttgaagga tttcgtttga aagaaattgc gactatcttg gatatgaatg aaaacacagt
    44161 taaaacgaaa atgtaccgct cattgaatgc tttacgtcaa atcctagaat gaaggagaga
    44221 agagtgccat gtattcccaa aaagaaaatt taaaaaaact aaagaaaact tataaagaac
    44281 aagcaattcc caaagggctt aaagaggaga tccgccagcg ttttattatc gaagagcaat
    44341 catttataaa agcaaagcgg agaaagagac gtctccaagg gttcggagta tctttggcta
    44401 tcgtaatgat tacaacaagt agccttcttt ttaatagtca agttcgaagc tttgcagaag
    44461 atttacccat cttaggatca gtcattgaat tgatcttagg cgaacgcttt actgatcgat
    44521 ctgaaaaaat cgatattcag gtaccgaaga tcaatacaca aaatgagaaa gaaaataaaa
    44581 caattcatgg attaaatcaa aaatactttc gcgaaggaca agcagatttt gaaaaagctg
    44641 aaaaagaata tggaaatttt gaaacggatc attaccaagt tttgggagat tatcagaaaa
    44701 tcgtagacga taaccggttt ctcgtgattg aaaggcagat cacccaaaca gcagcagata
    44761 gccatgttga aaaaagatac gacacaatcg acaagaaaaa cagtgtccag ctctcacttc
    44821 cacttttatt caaagatgac acctatctat ctgttttgac aaaagaagtc aaacggcaga
    44881 tggccgaaca agtcaaggaa gatccatcta agtactattg gactgagcaa gatattcaag
    44941 aagggacgat agaaaaacca acacttgtta cgcctacacg aagtttttat ttgaacaaag
    45001 agcatcaatt agttttgact ttttctcaat acgaaattgc tcctggttat atgggtacac
    45061 cagaatttgt cattcctaag tccgtgacaa aaacgatttt ggcatcggaa gattatttgg
    45121 atcattagac gagaagtagt tgcgactagc agtttcaatt ttcaagtaag tacaagtaaa
    45181 taataagaag agaaaaagag aaagaagaga tatagtgaaa agaaattatt tagttatcag
    45241 tattattgct ttatccgtgc ttttgttttt cgaaattact tcggtggcct ccttgatcac
    45301 cgaagtaaaa ggacaaacac actcttcgga gcattcgtca caatctttca aacaatcaga
    45361 acaatcaacc attaaaagcc aatcaactga aacagctaac gtcaaagaag ccattcaaga
    45421 atcagcggaa aaggtacaaa gtcagtcatc acaatcaaca caagacagta caaaaacgag
    45481 ccgagatccg gctgtcaaaa gcgtcgcaat cagctttgat gatggaccag gagcgaccac
    45541 aacaccgcag cttctgcgaa tcttaaaaga aaaaaacgtg catgccactt ttttcgtctt
    45601 aggagaaaat acggcgcagc atcctgaaat cgtgaagcag acagctgaag cagggcacga
    45661 gattggcaat cacacatacg atcatcagaa tctggccact ctttctgccc agtcgatgac
    45721 ggaggaagtg acgaaagcag acactgagat caaaaaagtc actggcaaaa cccccacttt
    45781 tgttcgtccg ccctatggtt ctgtcacgaa tgtaggagca acgatcatcc agcgaccgat
    45841 catcgaatgg tctgttgatt cagaagattg gaagacaaga aatcctgatt tgattttgca
    45901 gaagatccaa gcaacagtat acgatggggc aattatttta ttccatgata tctatcctga
    45961 aacgattcgt gccgttcctc aagtgatcga ttatctgaaa gagcaagggt atcgcatcac
    46021 gacagttggg gatttattag ggcatccaac ggctgtcgag aactattatg gcagaaacga
    46081 tcatcggcca gttcagtaag tgagacaagt atctttaata aataacacat tttaataagt
    46141 agttgaacac ataactacct tacttgcaaa ggcttaacaa tatctataca ggtacaacaa
    46201 aaaactgtcc accaattttc tttggcggac agttttcact taaaaataaa gaagcaacta
    46261 ttcgtaaatt atgctaaaat agcagtagaa cgcgtcctga gggaatcgaa cccccgtctc
    46321 aagaaccgga atcttacgtg atatccacta cactaaggac gcaagcactg ggataattgt
    46381 aactaaacag ataaaaaatt acaaggggat tggacaaaaa aatgaaattt gagcaaaatt
    46441 tttcacaaaa acagcagcag acacagaaac tagccatgac gcaacagttg caacagtcca
    46501 tccaagtttt acaatataac agtgaggagc tgttggcatt cgttgagaat caagcaatgg
    46561 aaaatccatt ggtggaagtt gttgagccgg aatggcagcc agattacata aaagcttctt
    46621 catcttctta tgaaggagaa gagacaaatt acctaaatca gattcctgat acaaaagggt
    46681 ctttatttga ttcattgatc gaacaagtcc atttgaatta ccgagatact tttttgagga
    46741 aaatcgttct ttatcttgta gaatacatcg atttgaacgg atttttgacg attagtttag
    46801 aagaagccgg aaaagagatt ggagctacgc ctatccaaat gctcgatgcg ttgactctga
    46861 ttcagcaatt agaaccagct ggagtaggtg cacgtagttt gcaagaatgt ctcatgcttc
    46921 aaacagaacg agatgattat gcgccggaat tagcttatat cgtgttggaa gaatgttttg
    46981 aggaactggt ggagcgaaaa tggaaagaaa tcgcacaacg tttttctgtt gatctacatg
    47041 ctgttcagca gatattcgac tatttacaaa cgttgagtcc aagccctggc cggattttcg
    47101 accgtagcag cgaattgtac attattcctg atgtccgggt attagtggac gacgataaaa
    47161 atgtacaagt tatctcaaac cgaaaaaatc agccaaatat acgctttcaa gagagttact
    47221 tcaaacaaat gtcacaacaa gcagataaag aaactgaaaa ttatttaaaa gaacggaaac
    47281 aggaattcga atggttgaag aaaacgatcc tacaacgtgg ggatacaatt ttgcgggttg
    47341 ctcaagtcat tgtttcccgg caaaaagctt tctttatcga caaggaacgt ccaatcaagc
    47401 ctcttacgtt aaaggaagtt gcaactgaga tagatgtgca tgaatccacg gtcagtcgtg
    47461 cagtcaacgg gaaatatttg gaaacagact tcggtgtatt tgaattaaaa aaatttttca
    47521 ctacacgaat tcctacgaat agtacagaac agacagaaga cctttcagca gatacagcga
    47581 agaaaaagct gcaggaatta gtcgatcaag aagacaaaaa caagccgttg tctgaccaga
    47641 aattggttga attgctgaaa aaagatgaga tagcgatctc aagacgaaca gtagcaaaat
    47701 atcgtgattt attaggaatc ccgagttcat caaaaagaaa acgttatgat aactaatcag
    47761 tcaaaagact agaaaaagta tttcactttt tctagtcttt ttagttgaaa aacgagagca
    47821 aaactgttag acttacagtc gtgaggttga gatttccctt tattgatgtg aaattcgaat
    47881 agttaatttc tttttttatc gtatatgggt cgttttaagt ctacattgga cgtaaagtgt
    47941 ccaactaagg agatcgctat gctggatgaa ataaaaatga ttgaatctgt tgcaccagat
    48001 attatcgaag tgttgcaaga acgctataaa attttacgaa atatctattg gatgcagccg
    48061 attggacgtc gaagtttatc ggaaagtatg gggatcacag aacgagtatt gcgaacagaa
    48121 acagatcttt tgaaaaatct gaatttgatc gacacctcaa aaagcggtat gacgctgaca
    48181 actaagggcg aagaagttta tcaaagcctt gaaaacttca tggatcagct tttaggtacg
    48241 catcaaaccg aacaacagtt ggcagaatat tttggtaacc aacgctgtat cgttgtttca
    48301 ggaaacagcg aagagcagac caaagtggct gatgcattcg gcgaagtgct gagcgaagcc
    48361 ctagaccgac tacttccaga aggcgagaat atcatcgctg tgatgggagg aactactatg
    48421 gctgtggtag ccgaacaatt gtctaatctg gaaaataaga aacggcataa cctgtttgtc
    48481 cctgcaagag gcgggattgg tgaagcgata actgtacagg caaattctgt cagtgcaaga
    48541 atggctgcta aagcaaacgg taaccaccgt gcactttatg ttccggaaca attaagtctg
    48601 gcaacttaca attctttact caatgagcca tctatccaag aagtcttaaa cttgattagt
    48661 gaagcgaatt gtgttattca tagtattggg cgtgcattac acatggcagc acgtcgtaag
    48721 atgactgaaa aagaattagt catgctgaaa caagcaaatg ctgttgcgga atcttttggc
    48781 tactttttca atgaaaaagg tgaagtcgtt tataaggtcc ctagaatcgg catagaattg
    48841 caagatttgg aaaaagtccc gatcatcatg gcaatcgcgg gcggaaaatc aaaagctaaa
    48901 gcaattcgag cttatatgaa aaatgcacca aaacaaacgt ggctcatcac tgatgaagct
    48961 gctgcaaatg agattttaaa aggggtaacc ctttaaaata aatatttttt tgattttcat
    49021 aaggaggaaa tcttaaatga cagttaaagt aggtattaat ggttttggac gtattggacg
    49081 tctagcattc cgtcgtatcc aagatgtaga tggaatcgaa gtagtagcaa tcaacgactt
    49141 gacagatgct aaaatgttgg cacacttgtt aaaatacgac acaactcaag gacgtttcaa
    49201 cggaacagtt gaagttcatg aaggttcatt caacgtaaac ggaaaagaag ttaaagtatt
    49261 agctaaccgc aacccagaag aattaccatg gggcgaacta ggcgtagata tcgttctaga
    49321 atgtactggt ttcttcactt ctaaatcagc agctgaaaaa catttaacag ctggtgctaa
    49381 acgcgttgtt atctctgctc ctggcggaaa cgatgttcca acaatcgttt ataacacaaa
    49441 ccacgaaaca ttaactggta aagaaacagt tatctcaggt gcttcatgta ctactaactg
    49501 tttagctcct atggctaaaa cattgaacga caaatttggt gttgttgaag gattaatgac
    49561 aactatccac gcttacacag gtgaccaaat gactctagac ggacctcatc ctaaaggtga
    49621 cttccgccgt gcacgcgctg ctgctgcaaa catcgttcct aactcaacag gtgctgctaa
    49681 agctatcggt ttagtaattc ctgaattgaa cggtaaatta gacggagctg ctcaacgtgt
    49741 tcctgtacca acaggttcat taacagaatt agtaacagtt cttgaaaaag aagtaactgt
    49801 tgacgaaatc aatgcagcaa tgaaagaagc ttcaaacgaa tcttacggat acaacacaga
    49861 cgaaatcgtt tcttcagata tcgttggtat gacttacggt tcattatttg atgctacaca
    49921 aactaaagta atgacagttg gcgacaaaca attagttaaa actgttgctt ggtacgacaa
    49981 cgaaatgtca tacactgcac aattagtacg tactttagaa tacttcgcta acttataaga
    50041 tagacaaacg tacaagtgta aattatagac atgtacattt aaaagcgggg aagcaatcgc
    50101 gcttcttccg ctttttttga taataagttt attcaggagg aaataaaatg gctaagaaaa
    50161 cagtgaaaga tatcgattta aaagacaaaa aagttcttgt ccgtgttgac ttcaacgttc
    50221 ctttgaaaga cggcgtgatc actgacgaca ctcgtatcaa agcagcattg ccaacaatca
    50281 actacgttct agaacaaggc ggaaaagcaa tccttttctc tcaccttgga cgtgtaaaaa
    50341 cagaagaaga taaagaagga aaatctttag ctccagttgc aaaacgtcta ggcgaattat
    50401 taggtaaaga agtaacattc gtacctgaaa ctcgcggaga acaattagaa gaagcaatcc
    50461 gcaacatgaa tgacggcgac gttgtcgtat tcgaaaatac acgatttgaa gatattgacg
    50521 gtaaaaaaga aagcaaaaat gatacagaac taggtaaata ctgggcttca ttaggcgatg
    50581 tatttgttaa cgatgctttc ggtacagctc accgtgcaca tgcttctaac gtaggtatcg
    50641 cttcaactgg tatcccaaca gttgctggat tcttgatgga aaaagaaatc aagttcatcg
    50701 gtgaagcagt agaagaacca aaacgaccaa tgattgcaat ccttggtggc gcaaaagttt
    50761 ctgataaaat cggcgtaatc gaaaacttga ttcctaaagc agataaaatc ttgatcggtg
    50821 gcggaatgac ttacacattc tacgaagcaa aaggaatcaa gatcggtaac tctctagtag
    50881 aagctgataa agtggaacta gcaaaagaat taatcgaaaa agcaggcgac aaacttgtct
    50941 tgccaatcga caatgtctgt gcaccagaat tttcaaatga tgttgaaaca caagtaatcg
    51001 aaggagacat tcctgatggt ttgatggctt tagacatcgg cccagcatca gtaaaattat
    51061 ttgctgatac attacaaggt gcaaaaacag ttgtttggaa cggacctatg ggtgtatttg
    51121 aaatgagtaa ctttgctaaa ggtacaatcg gtgtttgtga agcaatcgct aacttagaag
    51181 gcgcaacaac aatcatcggt ggcggagact cagctgcagc agcagaacaa ttaggatttg
    51241 ctgacaaatt cacacacatc tcaacaggtg gtggcgcaag cttggaattg cttgaaggta
    51301 aagaattacc aggactagca gcaatcaacg ataaataaac gaccaagttt gctcaattct
    51361 gacgagtaat cgatcaaaag gtgaagggag ttttcatgac tccctagcca tttttcaata
    51421 aattaaggag tgtgttcctc aatgcgtaaa cctattatcg caggtaactg gaaaatgaac
    51481 aaaactgcat cagaagcaaa agaatttgct gaagcagtaa aaaacaaaat cccttcaaat
    51541 gacaaagttg attcagtcat cggatctcct gcattattct tacaagaatt aatgacatca
    51601 gctgaaggta cagaactaaa aatctcagca caaaactgct actgggaaaa ctcaggtgcg
    51661 ttcactggtg aaacttcacc agcagctctt gctgatctag gtgttcatta tgtgatcatc
    51721 ggacattcag aacgtcgtga atatttccat gaaacagacg aagacatcaa taaaaaagca
    51781 aaagctatct ttgcaaatgg tatgacacca atcctatgct gcggcgaatc tttagaaacc
    51841 tatgaagcag gcaaaactgc tgaatggatc gaaggacaaa ttacagctgg tttagctgat
    51901 ctttcagctg aacaagtgtc aaacatggtt attgcttatg aaccaatctg ggcaatcggt
    51961 actggtaaat cagctgacgc acaaatcgct gacgaaattt gtggagttgt acgtcaaaca
    52021 gttgaaaaac tttatggaaa agaagtatca gaagcagttc gtatccaata cggcggttct
    52081 gtaaaaccag aaaatattgc tgaatacatg gcaaaagaaa atgttgacgg tgctttagtc
    52141 ggtggcgcaa gcttgcaagc agattcattt ttgtcattgt tagacgctgt aaaataattg
    52201 ttgaaaattt gcgaaaaatg caagaaatgt ttgcactaat gagcaaaatt aactaaaatc
    52261 gtatatgagg gaaatccctt aggaaaaaac aaactcaaag gagagacaaa acatgtcaat
    52321 tattactgat gtttacgctc gcgagatctt agactcacgt ggtaacccaa caatcgaagt
    52381 agaagtatac acagaaagcg gagcttttgg ccgcggaatg gttccttcag gtgcttcaac
    52441 tggtgaatac gaagcagttg aattacgtga cggagacaaa gctcgttatg gcggtaaagg
    52501 tgtaactaaa gctgtcgaca acgtaaacaa catcattgct gaagcaatca ttggctacga
    52561 tgttcgtgac caaatggcta tcgacaaagc tatgatcgct ttagacggaa ctcctaacaa
    52621 aggtaaatta ggtgcgaacg ctattttagg tgtttctatc gctgttgcac gtgcagctgc
    52681 tgactaccta gaagtacctt tataccacta cctaggcgga ttcaacacaa aagtattgcc
    52741 aacaccaatg atgaacatca tcaacggcgg atcacatgct gataactcaa tcgacttcca
    52801 agaattcatg atcatgcctg ttggcgctcc aacattcaaa gaagctctac gtatgggtgc
    52861 tgaagtattc cacgcattag cttcaatctt gaaagctcgc ggattagcaa cttctgttgg
    52921 tgacgaaggt ggatttgctc caaaccttgg ttcaaacgaa gaaggttttg aagtaatcat
    52981 cgaagcaatc gaaaaagctg gctatgtacc tggtaaagac gttgttcttg ctatggatgc
    53041 tgcttcttca gaattctacg acaaagaaaa aggtgtttac gtactagctg actcaggcga
    53101 aggcgaaaaa acaactgacg aaatgatcaa attctacgaa gaattagttt ctaaatatcc
    53161 aatcatctca atcgaagatg gattagacga aaacgactgg gacggattca agaaattaac
    53221 tgacgtatta ggcgacaaag ttcaattagt tggtgacgac ttgttcgtaa caaacactca
    53281 aaaattatct gaaggtatcg aaaaaggaat tgctaactca atccttatca aagtgaacca
    53341 aatcggtaca ttaacagaaa ctttcgaagc tatcgaaatg gctaaagaag ctggctacac
    53401 agcagttgta tctcaccgtt ctggtgaaac agaagattca acaatctctg atatcgctgt
    53461 tgcaacaaat gctggccaaa tcaaaactgg ttctctatca cgtactgacc gtattgctaa
    53521 atacaaccaa ttactacgta tcgaagacca acttggtgaa gttgcagaat acaaaggttt
    53581 gaaatctttc tacaacttaa aaaacaaata attcttttct aatgaagata catgatcgac
    53641 tattgaaaga tagtcgagta tgatcactta gttaagtaat cgaactctct acttgctgaa
    53701 atatcagcag gtagagagtt tt
//
LOCUS       DACGLE010000011        50182 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0534
            SAMEA3304449-rid9560453.denovo.011, whole genome shotgun sequence.
ACCESSION   DACGLE010000011 DACGLE010000000
VERSION     DACGLE010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304449
            Sequence Read Archive: ERR985064
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 50182)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 50182)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702225.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702225.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 20:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,045
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 27 of 67
            Pseudo Genes (incomplete)         :: 25 of 67
            Pseudo Genes (internal stop)      :: 19 of 67
            Pseudo Genes (multiple problems)  :: 4 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..50182
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304449-rid9560453.denovo.011"
                     /strain="VREN0534"
                     /db_xref="taxon:1352"
     gene            complement(77..149)
                     /locus_tag="IXT20_000757"
     tRNA            complement(77..149)
                     /locus_tag="IXT20_000757"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(114..116),aa:Lys,seq:ctt)
     gene            530..1099
                     /locus_tag="IXT20_000758"
     CDS             530..1099
                     /locus_tag="IXT20_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HAQ4859227.1"
                     /translation="MEKMRQQLMEWGKELETFRLPHWEELPDLELYMDQVRTLVDRYL
                     SPVIQGEKHPLLTSSMVNNYVKLGLIPAPVKKRYNKEHVAFLLAITTLKQVLTIPEIK
                     EGILFQGKTVGIREAYNLFCDEQEAAVWMVSQLAQGKSHPQKFDQATPVEYIAVRAAT
                     LSFAMKLLAEKTIVLETEYLKEEKTNEKQ"
     gene            1086..1946
                     /locus_tag="IXT20_000759"
     CDS             1086..1946
                     /locus_tag="IXT20_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAQ4859228.1"
                     /translation="MKNNKIAILVDSGTDVPEELIKKYEMYMIPLKIIYKDQVYTDKI
                     DITSEEIYQRLPEEIPGTSLPDGESITEIFEQIKKDGYEKVLAVTISSGLSGTYNVVR
                     LIAQQQEELEAYVLDTKNIGIGAGFSAIQAAKWLEEGVEWDQLISNLNELVERTKVFF
                     NVATLEYLQKGGRIGLVASIVGTALKLNPIISCNEEGIYYTVGKARGRKKSLDKTVAY
                     VKEKIGNAKVFNLAVAHGDAKEEAIEMEARLKREFPQANQIYFGQISPALVVHTGPGL
                     LGVGVQVLEG"
     gene            2136..2840
                     /locus_tag="IXT20_000760"
     CDS             2136..2840
                     /locus_tag="IXT20_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ4859229.1"
                     /translation="MKKVLVVDDEKPISDIVKFNLAKEGYDVYTAYDGEEALEKVAEV
                     EPDLILLDLMLPKMDGLEVAREVRKTYDMPIIMVTAKDSEIDKVLGLELGADDYVTKP
                     FSNRELVARVKANLRRGATAAKEPEEAAPAELTIGDLTIHPEAYMVTKRGETIELTHR
                     EFELLFYLAKHLGQVMTREHLLQTVWGYDYFGDVRTVDVTVRRLREKIEDNPSHPNYL
                     VTRRGVGYYLRNPEQE"
     gene            2845..4680
                     /gene="walK"
                     /locus_tag="IXT20_000761"
     CDS             2845..4680
                     /gene="walK"
                     /locus_tag="IXT20_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism sensor histidine kinase
                     WalK"
                     /protein_id="HAQ4859230.1"
                     /translation="MKRKVRFFRSVNFKIAITFILILLISIEIIGAYFIRGLERSTIN
                     TFIKDMNQTVESLATTISPELNRKDNADDEEVNANIKRFIENSATSDIIEIRVVDEKG
                     IIRGTTDVNEQSAVGKKNDYVDINDFTTKRYVALDNDDKRVNINVQPILSPTGDAVIG
                     ALYVKSNIEQKYSEINNTAVIFFTASLIAAFISMIVSVLVARSITQPIGEMREQAIRI
                     ARGDYSRKVKVHGQDELGQLADTFNQLAERIEEAQDTMEAERNRLDSVLSHMTDGVIA
                     TDRRGKVITINDMAVSLLDIKNEEAIGQSILTLLDIEEEYTLRKLLEDPNEMVLDRST
                     STLESDQMILRVDFSMIRRESGFISGLVAVLHDVTEQEKTERERREFVSNVSHELRTP
                     LTSMRSYIEALSEGAWKDEEVAPNFLKVTLDETDRMIRMINDLLNLSRMDTGNTQLQL
                     EYVNFNELVNFVLDRFDMMVGNQEKNYKIRREFTQRDLWVEVDTDKIIQVVDNIMNNA
                     IKYSPDGGTITCRLLETHNNVVLSITDQGLGIPKKDLNRVFERFYRVDKARARAQGGT
                     GLGLAISREVIKAHRGAIWAESKEGKGSTFYISLPYEPYEEEWWE"
     gene            4677..5993
                     /locus_tag="IXT20_000762"
     CDS             4677..5993
                     /locus_tag="IXT20_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4859231.1"
                     /translation="MRISEKVVRIGLILMVALSIYFSYAIWLSPAGKSSINVDETNTQ
                     MIESQSNRKTSEVFLPLHVTWHHSGMIQETNSENLVYRLQLIIDKARFGKLSEVVSGD
                     KSQFEQVKNLNSGFELAYNAPFLLREYKEAFDLDLDVSNLAKESENMYFTRIQFDQGN
                     KKIRFLNYSNHLVYEANISLNWTDINKELTAKDTKWTEMTTEPSTIDTQYATKHSIKL
                     KKYSYILSQQPYTLFRNAFFQKPDSVKNNDESNELIFYGGNETMSIHSETQVVDFRGE
                     LPEKKENASVFSESFPYISKLGSSLGNLRYFDRSDNTIDYRIFVEGFPVFGTDSRGQI
                     GVEVGGETNGSVQVNIQTSLNTIQVPIPSDEEVELASTNEIIQQLKAKGADSNKIGSV
                     IVGYTWQNVKETNRVVDLLPEWYVKYKDNWYSANDLLEKLPGLEAN"
     gene            5994..6863
                     /locus_tag="IXT20_000763"
     CDS             5994..6863
                     /locus_tag="IXT20_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4859232.1"
                     /translation="MDFKKIEWIFFVAFLGLNVFLFSSYHDAVYQENNVIRSNQTESI
                     QQRLASDDITYTGKISSENKQGYYLSAEQTNLSTSLANYRKAGKSGLLASGVFLDGDV
                     LTKSLSSSDAEKNVIDPDKISSSLEDFLSNKDNILFGNDYVYTKNFSHLEEEPLEIQA
                     SQEYEGIPFNDDTAKIVLSLEKNDNDYSITKYMQTHLSDIEQLREKTELYTEEEAINT
                     LYINNKIPRGSKILWRQLAYSCILKVREKNVYVPVWHVAIETSDKVVQIESVNAFSNT
                     IVTNNTVPKVEDQ"
     gene            6918..7727
                     /locus_tag="IXT20_000764"
     CDS             6918..7727
                     /locus_tag="IXT20_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HAQ4859233.1"
                     /translation="MAEEKPAFKISILASGSSGNSLYIESEKKRLLVDAGLSGKKITS
                     LMAQIDRTPDQLDGILVTHEHRDHIHGVGVLARKYHLDVFANQQTWEAMEPLIGNVPV
                     EQKHLFEMGKVRTFGDIDIESFGVSHDAAAPQFYRFHKENRSFVMLTDTGYCSDYMRG
                     VIENADGYLMESNHDLEMLRMGPYPWNLKQRILGDRGHLSNEDGALVMTDVIGNRTKR
                     IYLGHLSKENNMKELAHLTMENVLKEKDFGVGHDFEIYDTDPDEATELFSI"
     gene            complement(7830..8459)
                     /locus_tag="IXT20_000765"
     CDS             complement(7830..8459)
                     /locus_tag="IXT20_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase"
                     /protein_id="HAQ4859234.1"
                     /translation="MKIALIGATGHAGSFILDEALSRDLDVTAIVRHPEKLPSDVPFI
                     QKDLFELTTTDLASFDVVVDAFNAPKGKEEMHQTSLSHLIEMLEGTDTRLIVVGGASS
                     LFLDTEKTTRMIDQVPKNAPFYPTAFNMYEGLLKLKQSKNLHWTYISPASLFDPHGKR
                     TGTYLLSDDYLQRNAAGDSTISMADYAIALVDEILDEKHEKQHISVVSR"
     gene            8629..9921
                     /locus_tag="IXT20_000766"
     CDS             8629..9921
                     /locus_tag="IXT20_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ4859235.1"
                     /translation="MKEWLDRAFLPMITSVVNLRPLTALRNGMTYTIPIIIVGSLFFL
                     FAHFPVDYIAQWIDKTGFSMYFTQVYQATFDVIAFWIVFAIAYAYAKEEDIDRLPVGI
                     TALSAYLLLIQPLNTQIDTVWTGAEGILGAVTTGLIIGWGYARLVRLAKGWKQREKIP
                     ENVWQSFTSLIPIIVIVTFTLIGSAVFQQLFRMTVVQGVWTYVQLPIQSFIDTLFGVL
                     LLGFFVPFLWLFGLHGSAMVNGLVSPVLQANSLANAEILASGQELTVANGGHIVTQQF
                     LDQFMTVTGAGLTLGAVFFMTVFAKSKKYRELGNLALLPAFFNINESIIFSTPIVMNP
                     MMAVPFIFAPVVSGLITYSALYFGFVPLFTAVQVPWTTPPILSGFLVGGVPAAILQGI
                     VLSISFFIYFPFLKKIDSANCKKERQTKNDGAAEKMND"
     gene            complement(9997..10659)
                     /locus_tag="IXT20_000767"
     CDS             complement(9997..10659)
                     /locus_tag="IXT20_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HAQ4859236.1"
                     /translation="MSLKKYSFLSIFCYGLIFISPLILASIGLVKSTSELITATAVMY
                     ILGAVVLAIFYFKQREPLAIESAVKKASAPKIVIYGLVGIFVALILQSIAVMLESVLF
                     GKATPSENTQNIIQMIMEAPAFILATTVAGPIMEEFVFRRSILGIISRYSNFWVGAVI
                     SSLLFAFAHNDGHLLIYFFLGFFFSLEYKATGRIWTSMITHVGMNTLVVLVQLAVQKG
                     LV"
     gene            10884..11168
                     /locus_tag="IXT20_000768"
     CDS             10884..11168
                     /locus_tag="IXT20_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HAQ4859237.1"
                     /translation="MLKPLGDRVIIEVAKEEEKTVGGIVLASAAKEKPQTGTVVAVGE
                     GRLLENGEKVPAAVKAGDQVMFEKYAGTEVKYEGKEYLIVAGKDIMAIVE"
     gene            11219..12844
                     /gene="groL"
                     /locus_tag="IXT20_000769"
     CDS             11219..12844
                     /gene="groL"
                     /locus_tag="IXT20_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736685.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HAQ4859238.1"
                     /translation="MAKELKFAEDARAAMLRGVDKLADTVKVTLGPKGRNVVLEKSYG
                     SPLITNDGVTIAKEIELEDHFENMGAKLVSEVASKTNDIAGDGTTTATVLTQAIVREG
                     LKNVTAGANPLGIRRGIELATKAAVEELHNISTVVDSKEAIAQVAAVSSGSDKVGHLI
                     ADAMEKVGNDGVITIEESKGIETELDVVEGMQFDRGYLSQYMVTDNDKMEAVLENPYI
                     LITDKKISNIQDILPLLEQILQQSRPLLIIADDVDGEALPTLVLNKIRGTFNVVAVKA
                     PGFGDRRKAMLEDIAILTGGTVITDDLGLELKDVTIENLGNASKVVVDKDNTTIVEGS
                     GEKEAIEARVQLIKNQIAETTSDFDREKLQERLAKLAGGVAVVKVGAATETELKELKL
                     RIEDALNATRAAVEEGMVSGGGTALVNVISKVSAVEAEGDVATGIKIVVRALEEPIRQ
                     IAENAGYEGSVIVDKLKNVELGTGFNAATGEWVNMVEAGIVDPTKVTRSALQNAASVS
                     ALLLTTEAVVADKPEPAAPAAPAMDPSMMGGMM"
     gene            complement(12933..14081)
                     /locus_tag="IXT20_000770"
     CDS             complement(12933..14081)
                     /locus_tag="IXT20_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAQ4859239.1"
                     /translation="MLKVWVEQTKDGQFKFIERYVDPYTEKTRKKSTTLTSNSPQAWK
                     KAQKILDKKIKEALENYNKSDITFGELYKEWYEYYKQHVKRTSYLKVPMMMKHVSKHI
                     SDDTIVRNIDETLINKMIEDMYTFGDLSLNYTKQTKTTLSVMLNYAIDRKYIQRNPAL
                     AVKIHPKKVEEEKRKLSMDKKYLEKEEIDQILKQLYSNPRRKLHGIIAEFLYLTGLRY
                     GELLALQMKDYEDGKISINGTLDYTSVKMDNAIKTTPKNTYSQREVQLPNRAKELIES
                     VIADNILAGRPTDPDQYIFISTSGTPLTLHSFNAILHKVEEELELEKSLSSHIFRHSH
                     VSLLSELGVPLKAIMERVGHSDANTTLSIYNHVTKRAKQQVIDKLNSL"
     gene            complement(14183..14767)
                     /locus_tag="IXT20_000771"
     CDS             complement(14183..14767)
                     /locus_tag="IXT20_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4352 domain-containing protein"
                     /protein_id="HAQ4859240.1"
                     /translation="MAKKVMGQDGKMYKVSKPFYKKVWFWVLAVILIIIIGSALNGGS
                     DSNKASDNGGEKVTKSSTSASSSKEEKSDTFYKIGDTVKVGDAEYTLNSVELTDERNQ
                     FEENQPAQVVKITYTVKNDGDSDIPVGTDVEVYGPDDKKSETYANENTMGSVAPGKQM
                     DVTAHFTLNQKGEIEIHFSPLVSFEKAAIFKATV"
     gene            complement(14894..15325)
                     /locus_tag="IXT20_000772"
     CDS             complement(14894..15325)
                     /locus_tag="IXT20_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HAQ4859241.1"
                     /translation="MYLKLKEMLSEYNLKVVYMEMKEPGFYYPKPRIIFLNENLYGET
                     AEAFHLSHELAHFSASHFEFSVLYDTSTTFHSKFETEADKIAILILLNIYIENELTDE
                     SQFNLEKFMEYYSIQNKLRYTCYAVCQCYFKKKYSYARQYV"
     gene            complement(15343..15663)
                     /locus_tag="IXT20_000773"
     CDS             complement(15343..15663)
                     /locus_tag="IXT20_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ4859242.1"
                     /translation="MITFERIKELAKKQGKSLNKVEEDLGYGKNVLYRLKNSNPSTER
                     LQEIADYFDVSVDYLLGREEKETPKHVDLSENDTVFSFDGKEISKETMRKAIAIAKAL
                     EENE"
     gene            16395..16652
                     /locus_tag="IXT20_000774"
     CDS             16395..16652
                     /locus_tag="IXT20_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase"
                     /protein_id="HAQ4859243.1"
                     /translation="MNKEMSQTIKVQKMIDDLTHGIDSQADKIIKELQGQKVKDAKML
                     LKTINFEMNPTKRKLADVLEEKLASAINEQELLFETDTFNS"
     gene            complement(16623..16931)
                     /locus_tag="IXT20_000775"
     CDS             complement(16623..16931)
                     /locus_tag="IXT20_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4859244.1"
                     /translation="MKPEFVITMLDGEKIKIFADTLVVGYDDASNIEEKDGMFYSSQI
                     YVNTLQGDIGVSTLATSRPLVGLIGFLSKATFFSLGDDIDSDETVIYKTSAVKSIGFK
                     "
     gene            17009..17755
                     /locus_tag="IXT20_000776"
                     /pseudo
     CDS             17009..17755
                     /locus_tag="IXT20_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311416.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor"
     gene            17770..17973
                     /locus_tag="IXT20_000777"
     CDS             17770..17973
                     /locus_tag="IXT20_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ4859245.1"
                     /translation="MTDIAEITQRDREKIKEYVESSKFLTYTMLAERFGISKSYLSLI
                     LNGKKTSAEANRIIDSIITMYEL"
     gene            18078..18377
                     /locus_tag="IXT20_000778"
     CDS             18078..18377
                     /locus_tag="IXT20_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4859246.1"
                     /translation="MKKPTLSELIEAAEKAVKPDDWYRQSLILEKFHGMSKTTLVEYC
                     KEMETIPEFAEGILRPGHSTTFIHYHTFIWFLKWKDANKYRVKSLSPSDVLKEAS"
     gene            complement(18606..19085)
                     /locus_tag="IXT20_000779"
     CDS             complement(18606..19085)
                     /locus_tag="IXT20_000779"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4859247.1"
                     /translation="MSRKIDNIILLTTIFYLVIGATLSLLSFLSNSNLIVLNQKSILT
                     EFAHIDYFSFFKTGLICLFLSSFILYIGTTFCLMVLNLKMFIIEKWIVIIAKIFSSIS
                     IIIPMTLTFSKEQFNVATTFVSFIALFSFAVPKTFQSKSSNAKVNVENTDRKNDESY"
     gene            19130..19828
                     /locus_tag="IXT20_000780"
     CDS             19130..19828
                     /locus_tag="IXT20_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage regulatory protein"
                     /protein_id="HAQ4859248.1"
                     /translation="MQELVILKNKEAVTTSLQVAESFEKKHQHVLRDIDALKKDVSNF
                     GQMFSEGNEPDSYGRNRRVFFISRDGFFLLAMGFTGKKAIYFKQKYIEAFNEMEDVIR
                     KNTVPQTIEDMMIYQLEEMKDVKKDVSMLKDTMRISGQQEFEIKQKGNMKVMEVLGGK
                     ESQAYEKISKKVFSKFWSEFKRTFSIPRYGELPRKRFDDAVSFIEMWLPETAIRMEID
                     QLNRQQRLFGDENE"
     gene            20015..20356
                     /locus_tag="IXT20_000781"
     CDS             20015..20356
                     /locus_tag="IXT20_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4859249.1"
                     /translation="MEKELSTLDQYLTDPSWGKSNIKETNNRKIRRNLLTDEELACDQ
                     DDLGNFVSIWDHVYLIHLSRKSKKPEYIYVIEDGLIDALEEYDRDNLIDISYYGSGKK
                     YIAEMEAEFDE"
     gene            20349..21020
                     /locus_tag="IXT20_000782"
     CDS             20349..21020
                     /locus_tag="IXT20_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1071 domain-containing protein"
                     /protein_id="HAQ4859250.1"
                     /translation="MSENKGTTTFEKLFSRKLNKILKKKGNFDYLSWAHAWEIMKKND
                     PQATVTINEYKHYRVVSGTHQDFLVEEYKPFLMDETGTYVSVSVTVKGHTETELFPVL
                     DYRNQPVVKPNAMQINNSLKRCFVKALALHGLGLYVFQGEDIPTPPRIDTKKLNMLET
                     ILEAFNEQMGKDMTKTLIEYVNEQTDKLGLLADNVETIEQLSYEQCALMERAIAAKRK
                     ELDKK"
     gene            21026..21712
                     /locus_tag="IXT20_000783"
     CDS             21026..21712
                     /locus_tag="IXT20_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4859251.1"
                     /translation="MFKPLIDSYSAVLKKFKGKDIGATINEEVNIERLKTMYDGYDGD
                     RIIEVRFIDPRRFTVQQRNFIYALIGDIFIDTGMPTDFWKEFFYFRFEGVTGRKISLK
                     DESNTTVSDANVLANIILDFIFEHHIPFKEGYEILPANQEYYFYKCITKRVCCICGKT
                     GADIDHFDKALGRRKRKEVDHSEYTFAALCRIHHTEKHKIGVINFKNKYQIKGIKLNQ
                     ETIKKLRIGG"
     gene            21719..22570
                     /locus_tag="IXT20_000784"
     CDS             21719..22570
                     /locus_tag="IXT20_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HAQ4859252.1"
                     /translation="MSDNKRYYYLKLKENFFDSDEMVLLESMPDGYIYSNILLKLYLR
                     SLKHEGKLMFNDRIPFNSTMLATITRHSVGVVEKAVQIFRDLQLIDVLDNGAIYMSDI
                     QSFIGKSSTEADRKREYRKKIEEAKRNLITGGQVSDKCPDKTTPELEIELEKDIELEK
                     DIENVTPSKKSKAKPVRHKYGEYKNVLLSDEQMEKLKIEFPNDYQERIERLSEYCESS
                     GKTYKNYLATIRSWARKEKSESKNASSGYKRTGRREKLPEWAIDQEAYLKKKALERAN
                     RQSKAPF"
     gene            22582..22851
                     /locus_tag="IXT20_000785"
     CDS             22582..22851
                     /locus_tag="IXT20_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4859253.1"
                     /translation="MKIDYLELINEIAKYKTGEEIEILRDVYDQLEEAGIEGIKNDRS
                     SWSKLRYYFALYIDTTQLRNLAYTKLLFVDCVKGLQKHLNELEQV"
     gene            23013..23315
                     /locus_tag="IXT20_000786"
     CDS             23013..23315
                     /locus_tag="IXT20_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4859254.1"
                     /translation="MDKKAAMKRIIELTHSENWQEDKEIVTEVQKLGKSMWTEKPKRR
                     TPRKIAIWHGDRILVTGTAEQLSEITGLSKNIIWDRARSLWIDSKGRQFRYVEEKK"
     gene            23312..23623
                     /locus_tag="IXT20_000787"
     CDS             23312..23623
                     /locus_tag="IXT20_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4859255.1"
                     /translation="MNELITKVEQWAKDKGLDQADPKAQFLKVAEEFGEIASAMARSN
                     DELFKDSVGDVIVTLIILSMQKGTNVQECLEMAYNEIKGRTGKMVDGVFVKSSDLEDS
                     K"
     gene            23620..23838
                     /locus_tag="IXT20_000788"
     CDS             23620..23838
                     /locus_tag="IXT20_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr domain protein"
                     /protein_id="HAQ4859256.1"
                     /translation="MIGLTIFWCAIGGLLAFLGYYLICESRKGRKYTVTGIVLLIIGI
                     LVAVGAELNLYVNGTQEDLVRFMFWMKN"
     gene            23849..24664
                     /locus_tag="IXT20_000789"
     CDS             23849..24664
                     /locus_tag="IXT20_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prohibitin family protein"
                     /protein_id="HAQ4859257.1"
                     /translation="MNENKLIKLGVAGAVIVGIGVIGGFKFFEKIDNGYVGVRYSMNG
                     GIKDEALTQGVKFVGIDKVIQYPIRLQTIQSKNISVSTSDGKKTTIDIKYDYKVDSTK
                     AAKMYKEFGNITSEDIESGWLKSKLQKVAREVYAKYSLLDVLSGDSSKVEAEVLTNFA
                     KSVESKGFEVEDVTLGVPDVDKETQKSIDAIIRAGQENEKAKLDAETAKTQADSEAYK
                     KTKAAEAEAESNRKVAESVTDNLIRYEEAQARKKHGWVTVNGADTVVTDEAGK"
     gene            24803..25159
                     /locus_tag="IXT20_000790"
     CDS             24803..25159
                     /locus_tag="IXT20_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1140 family protein"
                     /protein_id="HAQ4859258.1"
                     /translation="MDLITQYSDIILKKIMAKIQKDKKAKERAELVKLEMAETGVGVR
                     SSRHWKAAANIEFYYNEIQKGFDQMRELDRQTNWSKKLHQDRFKFVEKYKEILEEYLR
                     RTANDKKAHSIQHGFI"
     gene            25119..25364
                     /locus_tag="IXT20_000791"
     CDS             25119..25364
                     /locus_tag="IXT20_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4859259.1"
                     /translation="MIKKLIQFSMDLYDIESGATLSVESDHLIINFGGKRQIILWVVD
                     DVLFPEIVHDFEESKAVEFEIVKKVMELIEKYEEDSE"
     gene            25361..25780
                     /locus_tag="IXT20_000792"
     CDS             25361..25780
                     /locus_tag="IXT20_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein"
                     /protein_id="HAQ4859260.1"
                     /translation="MIPKFRAWDKRKNVMRDVAVLHFTKNGKTNFIEYWINPTELKSY
                     HVRNIDLMQSTGMKDKNGVDIFEGDIVLVSVRNGFDYLDNKVCIVKNSIDYSGLVCAT
                     VDEDLEYQIFNTELFEEYTYEVTGNIYENSELLEEQR"
     gene            25777..26334
                     /locus_tag="IXT20_000793"
     CDS             25777..26334
                     /locus_tag="IXT20_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1642 domain-containing protein"
                     /protein_id="HAQ4859261.1"
                     /translation="MNKQEAIKKLESIKAIGNDAIAACYNESINSGITLMKKIDEPQK
                     PVIPQLVAGWLEKSTDPFTKAEKIAYLIKSKDGDSYYFCDWFVRDGIVTQEQGEELLA
                     WATRQSYETLLSLYNGYEVEKEPLWAIKNADGNYLTKCALWGKDGVNYSFECNPSHRL
                     LFTDKATADAAALLVNGTVEEVVER"
     gene            26331..26627
                     /locus_tag="IXT20_000794"
     CDS             26331..26627
                     /locus_tag="IXT20_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4859262.1"
                     /translation="MKLKDGFYASSHGIGGLMLDMPTKNPKTRKKSKFKVGDMVRCEA
                     EEFVYPFRGYVKKILSNSAIIRIENTMKCDRSTAKHKHYLAVARLVDMEVIKSK"
     gene            26704..27117
                     /locus_tag="IXT20_000795"
     CDS             26704..27117
                     /locus_tag="IXT20_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autolysin"
                     /protein_id="HAQ4859263.1"
                     /translation="MQLLREVDFKQTRCNARDVLKNFRRLERMAGRSLIDIKSPIITD
                     MPKAPKHGNKAEDAIIQMMDIETERDAILAALMALSLISRQILYYSFCVPDSFSNYRI
                     SREVGYSERSIQRMKSEALIEFAEAYKHGKIIAYK"
     gene            complement(27575..27850)
                     /locus_tag="IXT20_000796"
     CDS             complement(27575..27850)
                     /locus_tag="IXT20_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4859264.1"
                     /translation="MYKPYMISYDLNNPGQKYDKVFEIIKEFGAYIKLQKSFWLVKTN
                     LNPNQMCEKLNTVLDNNDSLFICELQKNYQGRATEENWKFINEHIFS"
     gene            28304..28510
                     /locus_tag="IXT20_000797"
     CDS             28304..28510
                     /locus_tag="IXT20_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4859265.1"
                     /translation="MLGQCFGNHARFDSLPATLNAYDGYDYRKKIVKKLYGATYGNVV
                     SVLSVHHQASVTVAEVEDAAVNGE"
     gene            28706..28873
                     /locus_tag="IXT20_000798"
     CDS             28706..28873
                     /locus_tag="IXT20_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HAQ4859266.1"
                     /translation="MYRPQYLEQKYEVITVHKGNGEKVYEYRRPIKSDTYKRKENNEV
                     IPLYGKRIAKH"
     gene            28899..29243
                     /locus_tag="IXT20_000799"
     CDS             28899..29243
                     /locus_tag="IXT20_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HAQ4859267.1"
                     /translation="MTEEFYRWLLQLTREDRLVKFYQSPKWRRLREKAMKRDHYECQE
                     CRRLGKYHRVENVHHIKEVKDRPDLALDLDNLICLCVEHHNEVHGRYLTALDKQEKKI
                     ESFANFDASERW"
     gene            29248..29529
                     /locus_tag="IXT20_000800"
     CDS             29248..29529
                     /locus_tag="IXT20_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4859268.1"
                     /translation="MIINDNGREYDTEKIEEYSSYTQGLIKRLIYVRYVGIRDLLSDN
                     CCSKYKVNQVREALNKDNNVERIKNVFGYSIEEINYYIDFAEAFIPMVR"
     gene            29632..29946
                     /locus_tag="IXT20_000801"
     CDS             29632..29946
                     /locus_tag="IXT20_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HAQ4859269.1"
                     /translation="MFKNELSQNRYREKLRRSLISQLESQKTNIEPFLDNVDRYISLW
                     ETAISLEEDISENGIRLENGKKNESVALLVSVNKQMGLMLDKLAITPELVGEANESIP
                     EL"
     gene            29924..31618
                     /locus_tag="IXT20_000802"
     CDS             29924..31618
                     /locus_tag="IXT20_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HAQ4859270.1"
                     /translation="MNQFLSYKHIENWFKAIEEGTIKVCKEQLLLKNYLEERVFTRED
                     IYFDKQMVEDSINIPAQYFPFELIPWEKFLQCFIYGVRWKKDKTLVFNRYLSLMGRGN
                     GKTGFASWNNFFLLTAKHGIKNYDIDIYANNESQAKTSFDDVFKVIKDHPDLDKKVFK
                     ATKEVIQNIATNSKLRYNTANARTKDGKRPGANRFDEIHENEDYSMINVATSGGGKIR
                     DYREFYDTTNGHVRGGPLDDIIEESKMILSGELGIDKDGAEFSSLFPFICRLDNDNEV
                     DDPDMWEKACPTINYNADLKRKMFQEYSQMQRNAGLRLTFMTKRMNRPMEDTRFAVAS
                     YDDVLHTKEKEFPEKMDEVIGTVDFADRRDFASVGLLGKYDKDVYFTQHTFIHESALR
                     LQNIKREVIDISIDQGKSQIVHGKNIEADYIVGWFLEMSNKYYIKKIAMDMYRAKILK
                     PALEEAGFTVEIVRSGSVTHGMLKDLVDDLFINQRLFFGDDAIMRWYCMNVYEEHISN
                     GNIRYEKIEPETRKTDGFFSFLHGLNFLDDIYDSAPVTVTNSSVENTGTGFTPLVF"
     gene            31638..32816
                     /locus_tag="IXT20_000803"
     CDS             31638..32816
                     /locus_tag="IXT20_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HAQ4859271.1"
                     /translation="MGIFQKAVGYFTKKATVPLEEYFCKLQVDFVYRKFAIETCIDLI
                     ANAMSKAEFKSYEDGKNKKNDLYYRLNVAPNKKNNATEFRKKLIRRLIFYNEVLIVSP
                     SNNSSEIFIADSWDVTEYALKDDVFSQVQINNIVLDREFLESDVIYIKYADQQIRQLV
                     DAYYQAYGKLISSAMNVYKRSNARRYVLKGNLFRPQDNTTQDQINKMMTSQFKAFMEA
                     DNAGAVFQLQNEYTLEDFSGNFQSNSRDIKNLIDDIFEMTAAAFHVPKNLLKGDMSGL
                     SDQVDAFLMFEIIPIAELIQDAFNASLYEVEEYLSGNFVRVDTTMIKITSFKDLVDAI
                     DVGIRNGVFTINEGRERVGNDRSDKAMADEIFITKNNQQVSKGGEANDDNENISSSKE
                     "
     gene            32779..33465
                     /locus_tag="IXT20_000804"
     CDS             32779..33465
                     /locus_tag="IXT20_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease ClpP"
                     /protein_id="HAQ4859272.1"
                     /translation="MTTMKTFLAVKNEGAVPQIFIQGFIGSSWFFEGNTDKGIKNILD
                     SLGDQEEIEVVINSNGGDVFQGIAIGNLLKSNKAKVNVVINGLAASAASIIAMAGDTI
                     KIYNNAQLMIHRASTYGEGNVDDFRTIADQLESIDKSVKASYKTRFNGTDEALQELLE
                     KESFMDAETALSYGLVDEIIDAENSSGTEAKKEQSVEEILNDVKEKRAEKIAAFTAAL
                     NKTFGQGDVK"
     gene            33465..34625
                     /locus_tag="IXT20_000805"
     CDS             33465..34625
                     /locus_tag="IXT20_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HAQ4859273.1"
                     /translation="MTVKNLKGVTAASDQLMKAFKDGNEESFSAAMVSLSKEIQDKIL
                     EEATAKNQDQLVLMNRGQRVLTTQETKFYNEVVKNEGFAGVEELVPATVFERVFEDLE
                     QSHPLLQKITFVNTTGVTEWIVSRGVNPAWWGKLCEAVKKVLDNDFDVINMKQFKLSG
                     YIPVCKAMLDLGPVWLDRYVRTVLVESLRIALEQAIVDGTGKDMPVGMMRDMSKQTSG
                     EYAEKKAEPITALDAVTMGGLMARLSKFNIEGVNDPIYRNVNPSDVVLIVNPTDYWSK
                     VFPAKTVLTANGEYVQVLPVPVSDLQSTAVPEGKAVIGVASDYFMGVGSTLKIEASDE
                     YHFVEDERIYLAKQYANGQPKRNDSFIVLDISALGTTTTTTKPTTTTTTTQA"
     gene            34635..35510
                     /locus_tag="IXT20_000806"
     CDS             34635..35510
                     /locus_tag="IXT20_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4859274.1"
                     /translation="MKYILCQPAINRFKWEFEVCLTNLKKLGIKDIVLLFSRHDDQIP
                     IFFEKEYGVEVHVYDDLRDDKEYIPSIKPYLWWKYLEEDHSREDDRYFYIDSDVIFNK
                     RINLRKLPSKDDVWYCSDCCSYLSLDYIRSCENGENILKDMANIVNVTVESLETINTN
                     SGGAQWVINRPKANYWKKVYLDSNRLYRYLRGQKTNIQIWTAEMWAQLWNMMYFNIGP
                     KVHEELDFCFATDPIEKVKEVKILHNAGVTTNDEDLFFKGRYVTSTPFDEDLSFVNKK
                     KCSYAYVKAIKAVVR"
     gene            35507..35818
                     /locus_tag="IXT20_000807"
     CDS             35507..35818
                     /locus_tag="IXT20_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4859275.1"
                     /translation="MTPEQVTEELLIAVKDNIYVTWNEEDESIKKMIAKNAVYLQSKV
                     STTLSFSPESLEYGLLIERCRYDWNRALDEFEQNFASELLGFIQHYALQEYIAGDVNG
                     E"
     gene            35808..36161
                     /locus_tag="IXT20_000808"
     CDS             35808..36161
                     /locus_tag="IXT20_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HAQ4859276.1"
                     /translation="MANNRRLEETFNDGWLKILTQTTKRNELGKKIGVEDTEITSLKF
                     RNLSMRDSDITAMDAMGSKLTKKVKTPFHPIAKKFNKDQYFIVIDSMRYNVIYADYDN
                     FYIYFYLESVGEYGD"
     gene            36151..36552
                     /locus_tag="IXT20_000809"
     CDS             36151..36552
                     /locus_tag="IXT20_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4859277.1"
                     /translation="MVIDNSKEKERLNKQISAIKTSLEEHFGLKLFQDSVGEDELPDD
                     FNYFILETGEIEMITEPKYSVGQNLYLTFYSENREDLTGDSLDIISLIQNRSIRFQRM
                     DPNHLKLENQDRYIDQLVFTFRRLLKSDCHG"
     gene            36545..36949
                     /locus_tag="IXT20_000810"
     CDS             36545..36949
                     /locus_tag="IXT20_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4859278.1"
                     /translation="MAKNSWELKINGHDELLVRMERYSSESERLINEALKSKGSAIAV
                     DRITEKIPVSEADLRRGHQHAKNSRPLKTQYINLGFIIRPTRKFEYLKYPDLGIGTSK
                     RNQPDEFMRRGLGLALDPITELLIRQFDKLNK"
     gene            36961..37569
                     /locus_tag="IXT20_000811"
     CDS             36961..37569
                     /locus_tag="IXT20_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAQ4859279.1"
                     /translation="MAKTKTVVTTFDNVSIKRIAFNFKNAGNAIATNCNGQLDGETEM
                     QTVVKKCGATEVKSKSKPINMTVTITAHVPMEVYRRFNGLKQDGRIKPGIYSYGPDSV
                     GEDFSLAAEIVDDFEENRKLVGMLACTSNTGLTFSIENGADEVAALELEAKVMQDEFG
                     KFYHEAIVAELEEDLTDQWMTNLSADVIKKTSVVATTATQSQ"
     gene            37589..37951
                     /locus_tag="IXT20_000812"
     CDS             37589..37951
                     /locus_tag="IXT20_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4859280.1"
                     /translation="MNEDCSKIELNDGTILNLEPKLNIKKLLMINRDFNTDEFAKMSM
                     GKGSMDITVIQGAKAVYVAYRQANMVDYISFDEFIDKWDFDMEVAVAVYSTMMFKQAR
                     DAYQKEFEKANKEKKLQK"
     gene            37954..38136
                     /locus_tag="IXT20_000813"
     CDS             37954..38136
                     /locus_tag="IXT20_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4859281.1"
                     /translation="MPKLLVETWVDVYSMLTDVFSMPSDLVLSDICLDDILQMAYNKS
                     AYEGWKNYAINQSQKN"
     gene            38154..41474
                     /locus_tag="IXT20_000814"
     CDS             38154..41474
                     /locus_tag="IXT20_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAQ4859282.1"
                     /translation="MAKKRTEAEVTFIANDDGLKSTLKEISAELTKNRAELKLEQAQL
                     QQTGSESDKLGSKLSSLEKQYELQSQKVEVTSQRLANAKKYYGENSTEVQKLERELIN
                     QQTAQQRLSNDIDKTSKALAQSKGEFKTYESTMKDLDNEQQHLKSSASLVESEYKKWQ
                     ATAGQSATESEKLAKAQEYVGKQSDIAEQKIEVLKKQLDATQKEFGETSTEALEMKTK
                     LNDAEREFEELSNAAKNVDTSTVDDIGKKLDMGNLMEATDHLSVIGDKLIDVGSKSIE
                     AAGKAQAMQAQFKQVFGSLEGEAQDAVEGMAEEFGMLPNTIKPVFTQYTSMFKGLGYD
                     TKEAMELAGDSTQLAADAAAFYDKSMDDASESLNSFIKGNYEGGEQIGLFANDTQMAA
                     YAVKHNLIPATEGAKKASEESLLAVEKAQSKYADAIKKHGEGSLEAREAALKLKDAQD
                     KINEELGPQTQKWSDLDEATKQAVRVQYAEDMQKLAGATGQASRESDGLENQMTRAKQ
                     ALEDFYASLGEDILPVFIKGLQAGAKALQGLAEWWSKLDGPMKNFILALGGILALLST
                     LAPVITAVVTIVGTFGSTVLLPIIGIIAGVAAVIAIVITAFQNWGAITDWFSDLWKKF
                     TDWLGDTWESIKESASSVWDGVKETWSGFVDWVQEIWQGVSDWFGELWSGLVEGASNI
                     WQGVQETWQAFVDWVSNIWNGVKEVWSIIWADIVGIVQIPWTLITSLIQAGINIIVGI
                     FDVAGQLLGAAWQAVWTPISDFLKNTWDTMTQWISIAWNGIVTTFHTIFDPVVAWWNG
                     IWTSISTTASNIWNSISATASSIWNSIKNTITSLVQAAATVIQNIWSTVSSWLGGIWN
                     SISSTASNIWSSVTSSISNAINAAKSAIQSVWNSISSWISGIWNGIKNTALNLWNGIT
                     STISSKVNDGKNAISSGWSNLTGIVSDIFNNVKSTIANIWEGIKKTVSAPIDWIRDKI
                     SSVFDNLNISIPHIPLPHFKLSGEFNPLKGKIPTLGVDWYAKGSVFNSPNIIGVGEAG
                     PEAVLPLKRSVLQEIGDRILSSTSVSSRAQTIQPVNNYEFNLTIDGNADEVTMKQTTQ
                     QIIDSITKVQNDNASAWR"
     gene            41525..42262
                     /locus_tag="IXT20_000815"
     CDS             41525..42262
                     /locus_tag="IXT20_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAQ4859283.1"
                     /translation="MTDCIHSIIDGFPDYLHKLALAERPTIPSPKRQRVETSVLGRLG
                     GLVQDYSFEDMSFTLHYNYLEDVEDHQAFKQSFYIMRHWLNYAKKLEFSDDPNVYYVI
                     QTIDIGDAENDIVEWGEFDVNITAKPFARVQEDVPITVDKPQSFNLLNNSLEESFPKI
                     IITPSATSCQFTLNDYVFSFEGLVVGTDIVIDSDLMLCYEEQSDGDILDRSNKMKTMQ
                     YPTLQVDINHFNCTGLSKIQIYRNGLR"
     gene            42272..44563
                     /locus_tag="IXT20_000816"
     CDS             42272..44563
                     /locus_tag="IXT20_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4859284.1"
                     /translation="MIDNLLTIYDKNDANNLAEHLYDTQGLGALSDWLTATVSNKLNG
                     AEIFQGTYSINGTNADLIVEGRIIQCYVDENRAKQRLRIYYAKTSVIGNTIEVKAEPI
                     FNDIRKSVLNKYDSGTEKITATQAWQNAKVLAKPAIPSQFSFSSLVDTLANVKIEKAN
                     FLEFFGGKEGSILDRFHGEFLKDNNTLRHEKRLGTDHKIKAIYTKNLTGLDLEIDAQS
                     VLVGVYPFISSSSEGEDEITLPEEVIFTDYVDDYPAGYVSFVDFKDKATDVATLREAA
                     KDWLKTNIDKQKPQVSGSIELVPLRHQRGYEKFVDLEKVSMGDGVDVYHPQLKVNMSA
                     RIVEYTFNVLTNSYDKLVVGNVKTNFLENTENNVSNLINDAIDQLKNGGEISDLINDI
                     VDHQTDMITGQNGGYVLLDPKEAPSRILIMDTPDKNTARNVLQINNAGIGFSKTGING
                     TYETAWTLDGGFNASFITAGEIVGITIRGTTLISDGADYRTSIANGKMTWYSKKVNKD
                     IMELEARDYVSADAGIVSYTMKTGGGFMIRNPQGNLVFSTWDNGNNRPFLSFGAPNFR
                     YSNASYVTSGDGNSLGIDGSAGNSWEFKVAGRTMKFTSDGMLTLPGCFFGSWEDGKLA
                     RFEQSTVQVYKDFTVRGTKNSTVPTEHYGQRLLNAYETPEYYFADYGEAVTGDDGKVR
                     VDIDPMFAETVNLSRYMTHVTPTELVLCAVTHEDIDHFIIETSKPNVLVRWNLVAHRL
                     GYEDIRLKEDTAYDSTVLDQKRF"
     gene            44587..46767
                     /locus_tag="IXT20_000817"
     CDS             44587..46767
                     /locus_tag="IXT20_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4859285.1"
                     /translation="MASSLYNLALDFSKELNYTKAIMARQGDKGITVTVKPFLNGLQM
                     DTSGGTFTLKGTTPSNRYVDSVATSVTSEEVIFSLDGTFMSEAGYYKHCYVEYRKGNQ
                     ILTTQDIIFFSLGVSDISQGQADEYVSQLEELIRKYNETFDAFMAEIKGRVDSLNQQI
                     TDLTGQAKTLQDKLDALKEEISKLGNLQVMYSNSIDFGGYDYSGNPNLMANINADSFS
                     QGTGALSVVDDGDEVVVTLDPNHKLELFKAKSQPNLLIGKTYTMSVEIMLEDDFTGDP
                     SKIGLRYTKMPNWVSELYTRNTLTTAKGVWQKLTGTVKITATSDNAESWFIMLQNKDA
                     NNSLSGKLRLRHAKVEYGSTATPYQPNLFDAPYYLSKAALGENIANKSVTFPIKSSDY
                     AVYDYKNIENFVVGEKYVATIKATKPATQVFEVRNTNSYGFGAMKPVEGLTDIWVLEF
                     TPTRIDAASPSSLQIYQSPKNTKGAVQIDWLKIEKGDTRTPNIEQYKYRGIGMRDSNN
                     PKDYVWDLAPEYVENNLATDIKVAEITGKANNYTDGKVSEINSQLTASINEVDTTAKD
                     AQTKVNELDNKIDERINDTATTAFYGAQGEVSNKTKVAEYGVGTGYTTTAVGETHFER
                     QSNGYVKCLKAGKYSISAQIRVQLGGKYASWLYTDLYKDGTSVDNLVAYGVDALQNRF
                     AASGNIVTDLEVNDVLNMRTEIGVADGILQFTSCRTLVLTRLSD"
     gene            46845..47087
                     /locus_tag="IXT20_000818"
     CDS             46845..47087
                     /locus_tag="IXT20_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holin"
                     /protein_id="HAQ4859286.1"
                     /translation="MKDEAIQDVVERLVRIETKLDNYELLREKAESAKDRADQAYSIA
                     LNNAEDIKEMKANNKWSWGYMIGLGITIIGYFLTKL"
     gene            47103..47300
                     /locus_tag="IXT20_000819"
     CDS             47103..47300
                     /locus_tag="IXT20_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holin"
                     /protein_id="HAQ4859287.1"
                     /translation="MVLPDKYYQVIKWTVLTVLPAASVLVATLGKAYGWNGTDMTVLT
                     INAVATFLGVITGVSAYNLKK"
     gene            47311..48330
                     /locus_tag="IXT20_000820"
     CDS             47311..48330
                     /locus_tag="IXT20_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAQ4859288.1"
                     /translation="MKKKITITAMSLLTALFLLPINGFAYTINNEFNLGANEGSSQVA
                     NNQYILLHETANETATGRNEAQYMQRSWTSAYTAYIVGDGGIVYQVGQPGYVQYGAGS
                     YANANSPVQIELQHTHNKATFEKNYKAYVELARDSAKKYGIPLTLDTPYNQPGIKSHL
                     WVTQNIWGDHTDPYGYLSEMGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGAAN
                     PTLTDGTNYAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADGIY
                     RSDVNQAYNTSGNVGYHVSFNMRNFPSKKVYVMMRATNDPEGNTKGGAQDFHDKRWYL
                     NIPKR"
     gene            48520..48593
                     /locus_tag="IXT20_000821"
     tRNA            48520..48593
                     /locus_tag="IXT20_000821"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:48554..48556,aa:Ile,seq:cat)
     gene            48637..49578
                     /locus_tag="IXT20_000822"
     CDS             48637..49578
                     /locus_tag="IXT20_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Abi family protein"
                     /protein_id="HAQ4859289.1"
                     /translation="MSLKDDKPILRFNEQLEKLNDLGIYVADNEIHEAKNILSNHNYF
                     FKLLAYRKNYDKNEQKKYNISFLSIVDLAKLDMQLRYVLLPYCLDIEHSLKTYLMRLI
                     TNRKNKNGEYAENGYEIVKSVLADEDTPKDLKDQIFNGVRYRDSDGNLAFQEGFEKYY
                     NEPPIWVVLDLASISKIKFFVNYLANKQPNNNNLKMIKNNIGYVSYIRNSCAHNKPIL
                     LNLQRKSIIKNSVYANARRIGITHDEITNLNVARIFALFELHRIMCSEGMRSHRNKDF
                     SNYLERVNKTLELHEQNMHIKNFFNSLTKILDEFCVE"
BASE COUNT        17461 a         7894 c        10425 g        14402 t
ORIGIN      
        1 taaaaaaaga acagcgtaaa tgttttcgaa atgctttcgc accaacattt acctgttccg
       61 tgggtttggc acccaatggg ccgtgagggg ctcgaacccg cgacccgctg attaagagtc
      121 agctgctcta ccaactgagc taacggcccg tacaaaaagt acttaataat agtagcacta
      181 agaattttaa aatgcaagac tacatttaaa gaaaatcaga aaaatcttta cttccttata
      241 ggaaatcatt catagattat tcacatttgc tcgctattat ataagcatac caacaaacaa
      301 agttcttgca agagaacaaa ccctaacact tttttcattt tttactcctt tcccgccgtc
      361 tccccggcgg gtctcctgtt cttaacgata aaagacaaga aggcttagcc ttcttgtttt
      421 ttgttttcat ggaatcccgc taatcaaatc acctagttaa agcaactaga ttgaatgttt
      481 atcataagtc tgatatcatg tttatgaaat gaaaaaagga gcggttatga tggaaaaaat
      541 gcgtcagcaa ttaatggagt gggggaagga actagaaaca ttccgtctgc ctcactggga
      601 agaactccct gacttagaac tttatatgga ccaagtcaga acgttagttg atcgttatct
      661 ttcacctgtg atacaaggag aaaagcatcc attgcttacc tcttctatgg taaacaatta
      721 cgttaaacta ggtttgatac cagcaccagt taaaaaaaga tataacaaag aacacgtagc
      781 atttttactt gcaatcacta cgttgaaaca agtattgact attcctgaaa tcaaagaagg
      841 gatattgttc caaggaaaaa cagtaggaat tcgtgaagcc tataatctat tttgcgatga
      901 acaagaagca gctgtgtgga tggtcagcca attagcacaa ggtaaatctc atccgcaaaa
      961 attcgaccag gcaacccctg tagaatatat tgctgtaaga gcggcaactt tatcgtttgc
     1021 aatgaaactt cttgcggaaa aaacgattgt tctagaaacc gaatatttaa aagaggagaa
     1081 aacaaatgaa aaacaataaa atcgcaattt tagtagattc aggtacggat gtgccagaag
     1141 aattgataaa aaaatacgag atgtatatga tcccattgaa aattatttat aaagatcagg
     1201 tatatacaga taaaattgac attacctctg aagagatcta tcaacgtttg ccagaggaaa
     1261 tccctggtac atctttgccg gatggtgagt cgatcacaga aatctttgag caaatcaaaa
     1321 aagacggtta tgaaaaggta cttgcggtaa caatttccag tggattgagc gggacatata
     1381 atgtggttcg tttaattgca caacagcaag aagaattgga agcatatgta ctagatacga
     1441 aaaatattgg tattggtgca ggtttttctg cgatccaagc agcaaaatgg ctggaagaag
     1501 gagtagaatg ggatcaactg atcagtaatc tgaatgagtt agtcgaaaga acaaaagttt
     1561 tctttaatgt tgcgacattg gaatatcttc aaaaaggtgg acggatcggt ttagttgctt
     1621 ctattgtagg aaccgcttta aagcttaacc caatcatctc atgtaatgaa gaagggattt
     1681 attacacagt aggaaaagct aggggacgta aaaaaagttt ggataaaacg gttgcttacg
     1741 tcaaagaaaa aatcggtaat gcaaaggtct tcaacttagc tgttgcccat ggggatgcga
     1801 aagaagaagc aattgaaatg gaagcgagac ttaaaagaga gttcccacaa gcaaaccaaa
     1861 tctactttgg ccagatttct ccggcattag tagtccacac tggtccggga ttattgggtg
     1921 tcggtgtaca agttttagaa ggataaatca aaaaaaggaa acactgaggt gaaaatcagt
     1981 gttttccttt tttattgaag ttagaacaaa aaataataaa gtattttgca ctttcatttt
     2041 aattttctct tttgttttat aggcaaagat gaaacaaaag cagaaatacg gtataatgag
     2101 aaggtatgac aataatatat aagaggtggc tctttatgaa aaaagtttta gttgtcgatg
     2161 atgagaagcc gatttcggat atcgtcaaat tcaatttagc caaagaaggc tatgatgttt
     2221 atactgcata tgatggggaa gaagccttgg aaaaagttgc tgaggtagaa cctgatttga
     2281 ttttacttga tttgatgcta cctaaaatgg acggcttaga agttgctcgt gaggtacgca
     2341 aaacttatga tatgccgatc attatggtaa cagccaaaga ttctgaaatt gataaagtcc
     2401 taggcttaga gttaggcgcc gatgattacg tgaccaagcc attttccaac cgagaattag
     2461 ttgcacgtgt caaagcaaat cttcgtcgcg gtgccacggc tgcgaaggaa ccagaagagg
     2521 cagcacctgc ggaattgacg attggtgatt tgacgatcca tccagaagct tacatggtga
     2581 cgaaacgagg agaaacaatc gagttaacac atcgtgaatt cgaactactc ttttacttgg
     2641 cgaagcacct tggacaagtg atgacaagag aacatttatt acaaacggtt tggggttatg
     2701 attattttgg agatgtacga acagtggatg taactgtacg tcgtttgcgt gaaaagatcg
     2761 aagataatcc aagccatcca aattatttag tgacacgtcg tggtgtcggt tactatttgc
     2821 gtaacccaga acaggagtaa ttggatgaaa agaaaagtcc gcttttttcg atcagtcaat
     2881 tttaagatcg ctattacctt tattctgatt ttattgatct cgatcgaaat tattggtgcc
     2941 tactttatac gtggtttaga acgttccacg atcaatacat ttattaaaga tatgaatcaa
     3001 acggtggaat cattagcaac aacaatcagc ccggaattga atcgaaaaga taatgcagat
     3061 gatgaagagg taaatgcgaa tatcaaaaga tttattgaaa acagtgcgac tagcgatatc
     3121 atcgaaatac gggtcgttga cgaaaaaggg atcatccgag gaacaaccga tgtcaatgaa
     3181 caaagcgctg tgggaaagaa aaatgattat gttgatatca atgatttcac gactaaacgt
     3241 tatgttgctt tagataacga cgataaacgg gtgaatatca atgttcaacc gatcctgtcc
     3301 ccgaccggcg atgccgttat cggggctctt tacgtcaaaa gtaatatcga acaaaaatat
     3361 tcggaaatca ataacacagc agtcattttc tttactgcct cactaattgc ggcatttatt
     3421 tcaatgatcg tatctgtttt agttgctcgt tcgattacac agccgatcgg tgaaatgaga
     3481 gaacaagcca ttcgaatcgc tagaggagac tacagtcgga aagtcaaagt gcatggacaa
     3541 gatgaacttg gacagctggc tgataccttc aatcagttag ctgaacgtat tgaagaagct
     3601 caagatacaa tggaagctga acgcaatcgt ttggatagcg tgctatccca tatgacagac
     3661 ggcgtcattg cgacagatcg caggggtaaa gtcattacga tcaatgatat ggctgtttcc
     3721 ttgctagata tcaaaaacga ggaagcaatc ggacaatcga ttttgacact tttagatatc
     3781 gaagaagaat atacgttgcg gaaattatta gaagatccaa acgaaatggt acttgaccgt
     3841 tcaaccagta cattggaaag tgatcagatg atcttgcgag tagacttttc tatgatccgt
     3901 cgcgaatcag gttttatcag cggattagta gctgtcttgc atgatgtgac agaacaagaa
     3961 aaaacagaac gggaacgtcg tgaattcgtc tctaatgttt cccatgaact tcgtacaccg
     4021 ttgacaagta tgcgtagtta tatcgaagca ttgagcgaag gtgcgtggaa agacgaggaa
     4081 gtagcaccaa attttttgaa ggtaacgcta gatgaaacag accgaatgat tcgaatgatc
     4141 aatgacctct taaatctatc tcgcatggat acaggtaata cccagcttca attagaatat
     4201 gtcaatttca acgagttagt gaattttgtt cttgaccggt ttgatatgat ggtgggaaat
     4261 caggaaaaaa attataagat ccgtcgtgaa tttacgcaaa gagatctttg ggtcgaagta
     4321 gatacagata aaatcatcca agtagtcgat aatatcatga ataacgcgat caagtattca
     4381 ccagatgggg gaacgattac ttgtcgctta ttagaaacgc ataataacgt ggttttaagt
     4441 atcaccgatc aaggattggg gattcctaaa aaagatctga accgagtgtt cgagcggttc
     4501 tatcgagtag ataaagcacg tgcaagagca caaggtggga caggacttgg attagctatt
     4561 tctagagaag tgattaaggc gcatcgaggc gctatttggg cagagagcaa ggaaggaaaa
     4621 gggtcgactt tttatatctc gctgccatat gaaccttatg aggaggaatg gtgggaatga
     4681 gaatctctga aaaagtcgtt cgtataggac tcattttgat ggttgcgtta agcatatact
     4741 tctcttatgc aatctggctg agcccagcag gaaaaagttc aatcaacgta gatgagacaa
     4801 acactcaaat gatcgagtct caaagcaatc gaaaaacttc cgaagtcttt cttccgcttc
     4861 atgttacatg gcaccattcg ggaatgatcc aagaaaccaa cagtgaaaat ctcgtgtacc
     4921 gattgcaatt gatcatcgat aaagcaagat ttggaaagct ttctgaagta gtttcaggag
     4981 acaaatcaca gtttgaacag gtgaaaaatc tgaactctgg ttttgagttg gcctataatg
     5041 cgcctttttt gctaagagaa tacaaggaag catttgattt agatttagat gtgtcgaact
     5101 tggcaaaaga aagcgaaaat atgtatttta ctagaataca attcgatcaa ggaaataaaa
     5161 aaatccgatt tttgaattat tcgaaccatc tcgtatatga agcaaatatt tcgttgaatt
     5221 ggacagatat caataaagaa ttaacagcaa aagacacaaa atggacagaa atgacaacag
     5281 aaccatcgac gatcgacacg caatatgcta cgaaacattc gatcaaactg aaaaagtata
     5341 gttatatcct gtcacagcaa ccctatacac tttttagaaa tgcttttttc cagaaaccag
     5401 atagcgtgaa aaataatgat gaaagcaatg aattgatttt ttacggtggg aatgaaacaa
     5461 tgtctatcca ttcggaaacg caagtagttg attttcgtgg agagctgcca gagaaaaaag
     5521 aaaatgctag tgtattttct gaaagtttcc cctatattag caaattaggc agttctttag
     5581 ggaacctcag atattttgac cgcagtgaca atacgatcga ttatcggata ttcgtggaag
     5641 gcttcccggt ttttgggaca gacagcagag gacagattgg tgtggaagtt ggcggtgaaa
     5701 cgaatggttc tgtgcaagtg aatatccaaa ccagtttgaa tacgattcaa gtgccgatac
     5761 cttcagacga agaagtagaa cttgcaagta cgaatgaaat catccagcag ttaaaagcta
     5821 agggagcaga tagcaacaag attggttcag tgatcgttgg ctatacatgg caaaatgtca
     5881 aagaaacgaa tcgtgtcgtt gatctcttac ctgaatggta tgtgaaatat aaagataact
     5941 ggtattcagc taacgacctt ctggaaaaat tacctggatt ggaggcgaat taattggatt
     6001 tcaaaaaaat cgaatggata tttttcgtcg cttttttagg gttgaatgtg tttttattca
     6061 gcagttatca tgacgcggta taccaagaaa acaatgtcat tcgctctaat cagactgaat
     6121 caatccaaca acgtctggct agcgacgata tcacttacac ggggaagatt tcgtctgaga
     6181 acaagcaagg ctattatctt agcgcggagc agaccaatct tagcacttca ttagcgaatt
     6241 atcgcaaggc tggtaaaagc ggcttgctgg caagtggtgt gtttttagac ggtgatgttt
     6301 tgacgaaatc tttatccagt tcagatgctg aaaagaacgt gatcgatcca gataaaatta
     6361 gtagttcctt ggaagatttt ttatctaaca aagacaatat tttattcggt aatgattatg
     6421 tgtacacgaa aaacttttct catttagaag aagaaccact agaaatccaa gcaagccaag
     6481 agtatgaagg catcccattc aatgatgata cagcaaaaat cgttctttct ttagaaaaaa
     6541 acgataatga ttatagcatc acgaaataca tgcagacgca tttatctgat atcgagcagc
     6601 tgcgcgaaaa gacggaactg tatacggaag aggaagcaat caatacattg tacatcaata
     6661 acaaaatacc aagaggatca aaaattttat ggcgtcagct agcttattcc tgtattctga
     6721 aagtccggga aaaaaatgtg tatgttcctg tttggcatgt agcgatcgaa acaagcgata
     6781 aagtggtcca aattgagagt gtcaacgctt tcagcaatac gatcgtgaca aacaatactg
     6841 taccaaaagt ggaagatcaa taaaaaagcg agtataatga aaaaagttta caaagtaaga
     6901 agggaagtag gagtcaaatg gctgaagaaa aaccagcttt taaaatcagt atcctagcaa
     6961 gtggaagttc gggtaattca ctttatatcg aaagtgaaaa aaaacggctt ttggtcgatg
     7021 caggacttag cggtaaaaaa atcacttcct tgatggctca aattgatcgg acaccggacc
     7081 aattagatgg aatacttgtg acgcatgaac atcgagatca tatacatggc gtgggtgtct
     7141 tggctcgtaa gtatcatttg gatgtttttg ccaatcagca gacttgggaa gcgatggaac
     7201 cactgattgg aaatgtacca gtggaacaaa aacatctatt tgagatgggg aaagtcagaa
     7261 cattcggcga tatcgatatt gagagtttcg gcgtctccca tgacgcagcg gctccgcaat
     7321 tttatcgttt ccataaagaa aatcggtcat tcgtcatgtt gacggacacc ggatattgca
     7381 gtgattatat gcgaggtgtc attgaaaatg ccgatggtta tttgatggaa agtaatcatg
     7441 atcttgagat gttacgcatg ggaccttatc cgtggaattt aaaacagcgt attttaggag
     7501 atagaggaca tctttccaac gaagacggtg cacttgtcat gactgatgtg atcggtaatc
     7561 ggaccaaacg aatctatctt ggccatctta gcaaggaaaa caatatgaaa gaacttgctc
     7621 atttaacaat ggaaaatgtt ttgaaagaaa aagattttgg cgttggacat gattttgaaa
     7681 tctatgatac agatccagat gaagcaactg agctattttc gatttaaaca gttcaatcaa
     7741 taaaaatata aaaagcagaa gataaaatca atcagggttg gttttatctt ctatttttat
     7801 ttttttagca tattaaaaaa ataaccgtat tatcgactga ctacactgat gtgctgcttt
     7861 tcatgtttct catccagtat ctcatctact aatgcaatcg cgtaatctgc catgctgatc
     7921 gtactgtcgc cagcagcgtt tctttgtaaa taatcatcac ttaacaaata agtacctgta
     7981 cgtttgccat gtggatcaaa taaagaagct ggactgatat aagtccaatg tagattttta
     8041 gattgtttca attttaacaa tccttcgtac atattgaaag ctgttggata gaagggtgca
     8101 tttttaggta cttggtcaat catacgagtc gtcttttctg tatctaagaa taacgaagag
     8161 gcacccccaa cgacaattaa gcgtgtatcg gttccttcaa gcatctcaat caaatgggac
     8221 aaagaagttt gatgcatttc ttcttttcct ttgggagcgt tgaaagcatc aacgacaaca
     8281 tcaaaagatg caagatcagt agttgtcaac tcaaataaat ccttttgtat aaacggaaca
     8341 tctgatggca atttttctgg atgacgcacg attgccgtca cgtctaaatc tctggacaat
     8401 gcttcgtcta aaataaatga acctgcatgg ccagtagctc caatgagtgc aattttcata
     8461 aaaaacctcc ttttttattc ctgtccattg taagcagttg ctgaatattc gtcaaagaat
     8521 ccgatttttg aaaagctcag tgcgcattcc tttcatggtt cgtgatcatt gggtatagtt
     8581 aaattaagaa aactgattga taaaaataga agttgaagga gataagaaat gaaagagtgg
     8641 ttagatcgtg cctttttacc aatgataaca tctgttgtga atcttagacc cttaactgct
     8701 ttgcgtaatg gaatgactta cacgatccca ataattattg ttggctcgct gtttttctta
     8761 tttgctcatt ttcctgtgga ttacatcgct cagtggatag acaaaacagg attttctatg
     8821 tattttacac aagtatacca ggctacattt gatgtgatag ccttttggat cgtttttgcc
     8881 attgcatacg cttatgcaaa agaagaagac atcgatcgct taccagtcgg cataacagcc
     8941 ttgtctgctt atctactgct tattcagcca ctaaatactc agatagatac tgtctggaca
     9001 ggtgctgaag ggatactagg agcagttaca actgggctca tcatcggatg gggctatgca
     9061 cgtttagtaa gacttgcgaa agggtggaag caaagagaga agatacctga aaatgtttgg
     9121 cagtctttta cttcattgat tccaatcatt gttatagtca cttttacgct tatcggttca
     9181 gcggtttttc aacagttgtt cagaatgact gttgttcaag gagtatggac atatgtccag
     9241 cttcccattc aatcgttcat agacacgttg tttggtgtgc ttttattagg tttttttgtt
     9301 ccgtttttat ggttatttgg tcttcatgga tctgcgatgg tcaatgggtt ggttagtccc
     9361 gtcttgcaag cgaattcatt agcgaatgca gaaatcttag cctcaggaca agagttgacg
     9421 gtggcaaatg gcggacatat cgttactcag caatttctcg atcagttcat gacggttacg
     9481 ggtgctggat tgacgttagg ggctgtattt tttatgacgg tttttgcgaa atcgaagaaa
     9541 tacagagaat taggaaatct tgctctttta ccagcctttt tcaacatcaa tgaatcaatt
     9601 attttttcca caccgatcgt gatgaacccg atgatggctg ttccttttat tttcgcaccg
     9661 gtcgtttcag gattgattac ctattccgct ttatattttg gattcgttcc tttatttaca
     9721 gcagtccaag tcccttggac aacaccgccg atcctctcgg gtttcttagt aggcggagtg
     9781 cctgcagcga ttttacaagg tatcgtgttg tcaattagtt ttttcattta tttcccattt
     9841 ttgaaaaaaa tagattctgc aaactgcaaa aaggaaagac agacaaaaaa tgatggagca
     9901 gcagaaaaga tgaacgacta aaaaacaaaa atactcctgt cgctttagat tgggaaaatc
     9961 accaatctag gatgacagga gtatttatag tcatgtttat actagccctt tttggacagc
    10021 taattgaaca agaacaacta atgtgttcat tcctacgtgt gtgatcatcg atgtccagat
    10081 ccgaccagtt gctttgtact ctaggctgaa gaagaaacct aggaaaaagt agattagcag
    10141 atgaccatca ttatgagcaa acgcaaacag caaagagctg atcactgctc cgacccaaaa
    10201 atttgaatat cgactgatga tccccagtat actgcggcgg aatacgaact cttccatgat
    10261 gggtccagca acagttgtcg ccaaaataaa agcgggtgct tccatgatca tctggatgat
    10321 attttgtgta ttctcggaag gggttgcttt gccaaacaat acagattcta acatgacagc
    10381 gatgctttgc agaatcaatg cgacaaagat tccaaccaat ccataaataa caatctttgg
    10441 agcagaagct ttttttaccg ctgattcgat cgctaagggt tcacgttgct taaaataaaa
    10501 aatagctagg acaacggcgc ctagtatata catgactgca gttgctgtga tcaattcaga
    10561 cgtagatttt acaagtccta tcgaagctag gatcaagggt gagataaaaa ttagaccata
    10621 gcaaaaaatg ctgaggaagc tatatttttt taaagacatg taattcctcc ttttaaggga
    10681 taacaagcat gcaaggcaag cttatatcga ttgatttcaa ttatatcata atttcaataa
    10741 gcagctgtca gaataaaatt tcttttcttt gatttaaaag cttgcaaaaa agagggagaa
    10801 tgagtattat taaaaatgta ggttagcact cgaaggttaa gagtgctaac agattaaaaa
    10861 aagagttatc ggagggattt atcgtgttaa aaccattagg tgatcgcgtc attatcgaag
    10921 tcgcaaaaga agaagaaaaa actgtcggag gaatcgtttt agcttctgct gctaaagaga
    10981 aaccccaaac tgggacagtt gttgcagtcg gtgaaggccg tttgttagaa aacggtgaaa
    11041 aagttcctgc cgctgtaaaa gcaggcgacc aagttatgtt cgaaaaatac gccggtacag
    11101 aagtaaaata cgaaggaaaa gaatatctga tcgtcgcagg aaaagatatc atggcgattg
    11161 ttgagtaaat ttggaagata aaaaaataat caaaaataat caatgaggtg aatcaatcat
    11221 ggcaaaagaa ttgaaatttg cagaagacgc acgcgcagct atgttacgcg gggtagataa
    11281 attagcagat acagtgaaag taacattagg acctaaaggc cgaaatgtcg tactagaaaa
    11341 atcttacggc tctccattga tcacaaatga tggtgtgacg atcgcaaaag aaatcgaatt
    11401 agaagatcat tttgaaaaca tgggtgcaaa acttgtatca gaagtagcat caaaaacaaa
    11461 tgatattgca ggagacggta caacgacagc aactgttctg acacaagcaa tcgttcgcga
    11521 aggtttaaaa aacgtaactg caggtgctaa tcctctaggc attcgtcgcg ggatcgaatt
    11581 ggcaacaaaa gcagcagtcg aagaattgca taatatctca actgttgttg attcaaaaga
    11641 agccattgca caagtagcag ctgtttcttc gggttctgat aaagttggac atttgattgc
    11701 tgatgcaatg gaaaaagttg gaaacgacgg tgtcatcaca atcgaagaat caaaagggat
    11761 cgaaacagaa ctagatgttg tagaaggtat gcagtttgat cgtggttact tatcacaata
    11821 tatggtaaca gataacgaca aaatggaagc tgttctagaa aacccatata tcttgatcac
    11881 agacaaaaaa atctctaaca tccaagatat tctgccattg ttagaacaaa tcttgcaaca
    11941 atcacgtcca ttgttgatta ttgctgacga tgtagatggt gaagcattgc caactcttgt
    12001 attgaacaaa atccgtggaa cattcaacgt agtagctgta aaagctcctg gtttcggcga
    12061 tcgtcgtaaa gcaatgcttg aagatatcgc tattttgaca ggtggtacag taattactga
    12121 cgatctaggt ttagaattga aagatgtaac aatcgaaaac ttaggaaatg cttctaaagt
    12181 tgttgtagat aaagacaata caacgatcgt agaaggttct ggagaaaaag aagcaatcga
    12241 agctcgtgtg caattgatca aaaaccaaat tgctgaaaca acttctgatt ttgaccgcga
    12301 aaaattacaa gaacgcttag ctaaattagc aggtggtgta gctgttgtta aagttggtgc
    12361 agctactgaa acagaattga aagaattaaa attacgcatt gaagatgcat tgaatgctac
    12421 acgtgccgca gtagaagaag gaatggtctc aggtggtggt acagctctag tgaacgttat
    12481 cagcaaagtt tctgctgtag aagcagaagg agacgtggca acaggtatca agatcgttgt
    12541 tcgtgcttta gaagaaccaa ttcgtcaaat cgctgaaaac gctggttatg aaggatcagt
    12601 gattgttgat aaattgaaga acgtggaact aggcactggt ttcaatgctg ctactggaga
    12661 atgggtaaac atggttgaag caggtatcgt agacccaact aaagtaacac gttctgcctt
    12721 gcaaaatgcc gcttccgttt ctgccttgtt attaacgaca gaagcagtcg ttgcagataa
    12781 accagaacca gcagcgccag cagctcctgc aatggatcca tcaatgatgg gcggtatgat
    12841 gtagtattta atagagaacc ttgatatatc agggttctct attctttttt tgttttattt
    12901 taaacgaaag gggcaaagaa ggggcaaaaa tatcaaaggc tatttagttt atcaattact
    12961 tgttgtttgg ctcttttggt tacatggtta taaatagaca gagttgtatt tgcatcagaa
    13021 tgccctacac gctccataat ggctttaaga ggtacgccta attcagataa tagtgaaacg
    13081 tggctatgtc tgaatatatg tgaggataga cttttttcta attccaactc ttcctctact
    13141 ttatggagta tagcgttaaa tgagtgcagc gtaagcggag tgccacttgt agatataaat
    13201 atatattgat cgggatctgt gggtctacct gcaagaatat tgtcagctat cacactttca
    13261 atcaattctt ttgcacgatt gggtaattgc acttcacgtt gcgaataagt attttttgga
    13321 gttgttttta tagcattatc cattttcaca gatgtgtaat ctaaggtccc attaatggaa
    13381 attttcccat cctcatagtc cttcatttgc aaagctagca attccccata tctcaaacca
    13441 gtcaaatata gaaattcagc tattatgccg tgtagttttc tgcgaggatt ggaatataac
    13501 tgtttcagta tttgatcaat ttcttctttc tccagatatt ttttatccat agaaagcttt
    13561 cttttttctt cttccacttt tttaggatgg attttaactg ctagagcagg gtttctttga
    13621 atgtattttc tatcaattgc atagtttagc ataacggata gagttgtttt tgtttgcttt
    13681 gtgtagttca gtgagagatc accaaaagtg tacatatctt caatcatctt attaatgagt
    13741 gtctcatcaa tgtttctaac gattgtatca tcgcttatgt gcttagaaac atgtttcatc
    13801 atcattggaa ccttcaaata gctagtacgt ttaacatgct gcttataata ttcataccat
    13861 tctttataca gctcaccaaa agtgatatct gatttattgt aattttcgag tgcttcttta
    13921 atttttttat ctagaatttt ctgagctttt ttccacgctt gtggtgaatt acttgtaagt
    13981 gttgtagatt tttttcgtgt tttttctgta taaggatcta catatctttc aataaacttg
    14041 aattggccat ctttggtttg ttcaacccac acttttaaca tctcctatca tttgctataa
    14101 taggcataac aaatagacct atataggttt gttttctaaa agcacgctct tactctggac
    14161 ggtggggcgt gttttttatt tattatacag ttgctttaaa aatagctgct ttttcaaatg
    14221 atactaaagg agagaagtgg atttctattt ctcccttttg gtttaacgtg aaatgagcag
    14281 ttacatccat ttgttttcct ggagcaacag atcccattgt attttcgttg gcatatgtct
    14341 ctgatttttt gtcatctggt ccatatactt ccacatctgt acctacaggg atatctgaat
    14401 cgccatcatt ttttacggta taagtaattt ttactacttg tgcaggttgg ttttcttcaa
    14461 attgatttct ttcatcagtt agttctacac tatttagtgt atattcagca tcaccaactt
    14521 ttacagtgtc accaatctta tagaaggtat cgcttttttc ttctttagaa gatgaagcag
    14581 aggttgaaga tttagttact ttttcgccac cattatcact tgctttatta ctatctgatc
    14641 caccatttaa agcagaacca ataattataa ttaaaattac tgctaatacc caaaaccata
    14701 cttttttgta aaaaggttta ctaactttat acatttttcc gtcttgaccc ataacttttt
    14761 ttgccattta aatattcctc acttcttgtt ataatatatt tgcgatctca gaaatgaggt
    14821 atgagtccgt gtgccagcac gggctttttt aatgttttgc tgaaatcggt ttttttaata
    14881 attcttgatg tattcataca tattgccttg cgtaagaata ttttttctta aaatagcatt
    14941 ggcagacagc ataacaagtg tatctgagct tattctgaat agaataatat tccataaatt
    15001 tttcgaggtt aaactgagat tcatcagtca gttcgttctc aatatagata tttaataaaa
    15061 ttagaatagc tattttatct gcttctgttt caaattttga gtgaaaagtt gtagaagtat
    15121 cgtacaaaac tgaaaattca aaatgagagg cgctgaaatg tgcaagttca tgagataaat
    15181 gaaaggcttc tgcagtttcg ccatatagat tttcattcaa aaaaatgatt ctaggcttcg
    15241 gataatagaa gccaggctct ttcatttcca tgtaaactac tttcaaattg tattcgctca
    15301 acatttcttt caatttcaaa tacatacaac ccatcactcc aactattcat tttcctctaa
    15361 agctttagca attgcaatcg ctttacgcat tgtctcctta gatatttctt ttccatcaaa
    15421 agaaaaaaca gtatcgtttt ctgataaatc cacatgttta ggggtttctt tttcttcgcg
    15481 ccccagaaga tagtctacag agacatcgaa atagtcagca atttcttgca aacgttctgt
    15541 agaggggttt gagttcttaa gtctatataa gacgttttta ccatagccta ggtcttcttc
    15601 aactttattt aatgattttc cctgtttttt cgctaactct tttattctct caaacgttat
    15661 catatcaata ttcctttcgt tcaagaaaaa atatttaact ttatttgtta aaattcgttg
    15721 actattttaa caaaaggtgt taatatcatt cttgtaaaca agtttaacaa ctaaaaagac
    15781 aacaaaaaac actattgatt aataaatgcc aaccgccaag aaagctttta aaatcaatgt
    15841 ttatatgcct tatttaacta tgttctgatt ttaacactgt atgttaattt agtcaacgca
    15901 gttttttaaa aagttgttaa atttgtttac gaatataaaa gaaaggagag aagaatatgg
    15961 aaaaaacagt ctcggcaaaa atcgaagatt tgaaattaga catactgaaa caagcaaaag
    16021 tggcgatgga acacgcggta gataaagaag actctgccat ggttgcagcc atagcagaga
    16081 ttttagctca cgtttagtca tcgtttttac ggtcttctgg aagctgacca tagataacag
    16141 cataatgaat atctaaataa gcttgaacaa tttcttttgg cgaaattgat tcgcctttag
    16201 tagatacttg tgattcgtga tttagccatg caacaactaa atccgcagcg atttcaaacg
    16261 gcgttacact ttgtgtcagt tgccataaag aatttcacaa tatgtatggt cgtggtaata
    16321 ataccaaaga gcaatttgag gaatttatgg aaattaaaaa atacaaaaaa tcatttatta
    16381 aaggaggcta aacaatgaac aaggaaatga gccaaacaat caaggtacaa aaaatgatag
    16441 atgatctaac acatggaatt gatagccaag ccgataaaat catcaaggaa ttgcaaggac
    16501 aaaaagttaa ggatgcaaaa atgcttctca agactatcaa ctttgaaatg aatccaacaa
    16561 aaagaaagct cgccgatgtg ttggaagaaa agttggcttc cgctatcaac gagcaagaat
    16621 tactatttga aaccgatact tttaacagct gatgttttat agatgacagt ttcatccgaa
    16681 tctatatcat cgccgagaga aaagaatgtt gcttttgaca aaaagccgat aagacctaca
    16741 agcggtcttg atgttgcaag agtagatacc ccaatatcac cttgcaaggt attcacgtaa
    16801 atttgagaag aatagaacat tccatctttt tcttcgatat ttgacgcatc atcgtatcca
    16861 acgaccaaag tatcggcaaa tattttgatt ttttctccat caagcatagt aattacaaat
    16921 tctggtttca taaattttca cctccttaac aattatttca gcctgtcaca ctgataagga
    16981 aattatacca aagaaaggaa tgagaaatat gaatacacca caaattttta atttcgagca
    17041 aaacgaagtt cgaactattt tagtaaatga tgaaccatat tttgtaggaa aagatgtagc
    17101 agacgttcta ggatattcga atccgcaaaa agctattcga gatcatgtgg acttggagga
    17161 taagacgcag aacgattcgt tcaccgtcaa tggaacagca gttgttttga ttaacgagtc
    17221 aggcctttac agtttaatct taaaatcaaa acttccctct gccaaaaaat ttaaacgttg
    17281 ggtaacgagt gaagtccttc caacaattag aaaaacaggt agctattcaa acgtacctca
    17341 aagttttgca caagcattgc gtttagcagc ggatttagaa gaaaagaacc aattactcga
    17401 acaacaaatt gccgagtacg aaccaaagat tagctactta gatacgattc tttcatcgac
    17461 agatacggta gctacttctc aaattgcagc tgattacgga atgtcggcaa ttgctctaaa
    17521 caaattgcta aacgagttag gtgttcagca taaagttagc ggacaatgga tactttaccg
    17581 aaaacatatg aaccaaggat acacaaaatc gcacacaagt gagataccga aagccgatgg
    17641 cggcactaaa gttgtaatga ataccaaata gacacagaaa gggcgagtgt ttatttacaa
    17701 cttattaatc gcagagggct attaccctca aatggattta gaggaaattg gttagaaagg
    17761 agttttagta tgactgacat tgcagaaatc actcaacgag atagagaaaa aatcaaagaa
    17821 tatgtcgaaa gttcaaagtt cttaacttac accatgcttg ctgaaagatt tggaattagc
    17881 aaaagctact tatctttaat tttaaacggt aaaaagactt ctgcagaagc aaacagaatt
    17941 atagattcga ttatcactat gtacgaattg tagagggagg taaagtcgtt ggaagataaa
    18001 atcattctta caaaatcaga gcttcaagaa atgttaaaca tcgagtatgg aacgataatt
    18061 tttaggaggg gtaataagtg aaaaaaccaa cactttcaga gttgatagaa gctgctgaga
    18121 aggcagtaaa accagacgac tggtaccgac aaagtttaat cttggagaag ttccacggca
    18181 tgtcaaaaac tactttagtt gaatactgca aggaaatgga aacaattcct gaatttgcag
    18241 aagggatact tcgtccaggt cactcaacca catttattca ttaccatact tttatttggt
    18301 ttttaaagtg gaaagacgca aacaaatatc gtgtaaaaag tttatcgcct tcagatgttt
    18361 tgaaggaggc aagctaatgg gcaaattcaa cagagcacta gtattcagcg caccgctaat
    18421 catctacgct ttaggcctct ggggaagcag acaagcgttg ataggtacga ttgtgtacat
    18481 ggtttggatt tttatagggc tggatgaagc tgagtacaaa acaaaaaagc cagtcgggag
    18541 ggactgacta aaaaagtcta atgtctataa gaaaggtttg acaataaatg aaagaattat
    18601 tttttttaat aactttcatc attttttcta tcagtgtttt ctacattaac ctttgcgttg
    18661 ctagatttgc tttgaaaagt tttaggaaca gcaaacgaaa atagagctat aaaagatacg
    18721 aatgtagtag ctacgttaaa ttgctcttta gaaaacgtca atgtcattgg aatgataatt
    18781 gatatcgatg agaaaatctt tgcgataata actatccatt tttcaattat aaacattttt
    18841 aaattaagca ccattagaca aaatgtagtt cctatataga ggataaatga agatagaaat
    18901 aaacatatta gtccagtttt aaaaaaagag aagtaatcta tgtgggcaaa ttctgttaat
    18961 atgctttttt gatttaacac aatcaaatta ctgttggata aaaatgacag taaggaaaga
    19021 gttgctccga ttactaaata aaaaatagtt gttaacaata ttatattatc aatttttctg
    19081 ctcatgtttt caccccacca ggattatatc aaaaaggaga gaagaaataa tgcaagaatt
    19141 agtaattttg aaaaataaag aagctgtgac tacgagtttg caagttgcag aaagttttga
    19201 gaaaaagcat caacatgttt taagagatat tgatgcactg aaaaaagatg tgtccaattt
    19261 tggacagatg ttttctgaag gtaatgagcc agattcctac ggaagaaatc gacgagtttt
    19321 cttcattagc agagatggat ttttcttgtt agctatggga tttacaggaa agaaagctat
    19381 ttacttcaaa caaaaataca ttgaagcatt caacgaaatg gaagatgtta ttcgcaagaa
    19441 tactgttcct caaacaattg aagacatgat gatctaccaa ctagaagaaa tgaaagatgt
    19501 taaaaaagat gtctccatgc ttaaagatac tatgcgaatt agcggacaac aagagtttga
    19561 aattaagcaa aaaggaaata tgaaagttat ggaagttcta ggaggcaaag aaagccaagc
    19621 ttatgaaaaa atcagcaaaa aagtattctc aaaattttgg tctgaattta aacgtacctt
    19681 ttcaatccca agatatggcg agttacctcg taagagattc gatgatgctg tttcatttat
    19741 tgaaatgtgg ttaccagaaa ctgcgatccg catggaaatc gatcaactga acagacaaca
    19801 aagacttttc ggtgatgaaa atgaatagag ctgaagcgct aagaatcggg acggtaattg
    19861 ctaatcgctg gtggagacac aataaacaca ataaaccaat catcctaagc caacaacata
    19921 ttgacaagca aaaagcatgg caacaaataa aaaagtgact ccgccggcaa gcaaagagtc
    19981 acaaagaaaa cacatcataa ggagatttta gcatatggaa aaagaacttt ccactctaga
    20041 tcaatatttg actgatccta gttggggcaa atcgaatatc aaggaaacaa ataatcgaaa
    20101 aatcagacga aatcttttga cggatgaaga actagcatgt gatcaagacg atttgggaaa
    20161 ttttgtgagt atttgggatc atgtctatct tatccattta tcgaggaagt ccaaaaaacc
    20221 tgaatatatc tatgtcatcg aagatggctt gattgatgcg ctagaagagt atgacagaga
    20281 taacttgatt gatatctctt attacggatc aggtaagaaa tacattgctg aaatggaggc
    20341 agaatttgat gagtgaaaac aaagggacaa cgacttttga aaaacttttt agtcgcaagt
    20401 taaataaaat actcaagaaa aaaggaaatt ttgattattt atcttgggct cacgcatggg
    20461 agattatgaa aaagaatgat ccacaggcaa cggtaactat taatgagtat aaacactaca
    20521 gagttgtttc tggaactcat caagactttc ttgttgagga atataaacct tttcttatgg
    20581 atgaaactgg gacttatgta tctgtctcag taacggttaa aggacacacg gaaaccgaat
    20641 tatttcctgt tttagattat cgaaaccaac cagttgttaa gccaaatgct atgcaaatca
    20701 ataactcatt gaagcgatgc tttgtgaaag cattggctct acacggactg ggattatatg
    20761 tatttcaagg ggaagatatt ccaacaccac ctagaatcga tacaaagaaa ttaaacatgc
    20821 tagagacgat tctagaagct ttcaatgagc agatgggtaa agatatgacc aaaaccttaa
    20881 tcgaatatgt taatgagcag acagataaat tagggctctt agctgataac gttgaaacta
    20941 ttgaacagtt aagctatgag caatgtgcct tgatggagcg agcaatagca gctaagagaa
    21001 aagaattaga taagaagtga tatgagtgtt taaaccatta atcgattcat attcagcggt
    21061 tctgaaaaag ttcaaaggaa aagacatagg tgcaactatt aatgaagaag tgaacatcga
    21121 acgtttgaag acgatgtacg acggatatga tggcgatcgg attattgaag ttcgatttat
    21181 tgatcctaga cgtttcactg tacagcaacg aaacttcatc tatgcgctca taggcgatat
    21241 tttcatcgat acaggcatgc caacggactt ctggaaggaa ttcttctact ttcgttttga
    21301 aggtgtcaca gggcgcaaaa taagcctgaa agacgaatcg aatacaactg tgagtgatgc
    21361 caatgtctta gcaaatatca tcttagattt catctttgaa catcatattc ctttcaaaga
    21421 aggctatgag attttaccag cgaatcaaga atattacttc tacaaatgca ttacaaaaag
    21481 agtctgctgc atctgtggca aaacaggagc tgacatcgat cactttgaca aagcgctagg
    21541 aagacgaaag cgcaaagaag ttgatcattc agagtacacg tttgcagcac tctgcagaat
    21601 ccatcacacg gagaagcaca aaataggtgt gattaatttc aaaaataagt atcaaatcaa
    21661 agggatcaag ttaaaccagg aaacaattaa gaaacttaga ataggagggt aaaaaatatt
    21721 gtctgacaac aaacgctact actatttaaa actaaaagag aattttttcg atagtgacga
    21781 gatggttctc ttagaaagta tgccagatgg ctatatttat tctaatattc ttctcaaact
    21841 ttatttaaga agtctaaaac acgaaggtaa gttgatgttt aatgacagga ttccatttaa
    21901 ctctacaatg ctcgcaacta ttacaagaca ttctgtagga gtcgtagaaa aagcggtaca
    21961 aattttccgt gatttgcagc ttattgacgt attagataac ggagcaattt atatgtctga
    22021 tatacaaagt ttcattggaa aatcctcaac tgaagcagat agaaaaagag aatacagaaa
    22081 gaaaatagaa gaggcaaaac ggaatttaat aactggagga caagtgtcgg acaaatgtcc
    22141 ggacaaaact acaccagagt tagagataga gttagagaaa gatatagagt tagagaaaga
    22201 tatagagaat gtaacgcctt cgaaaaaatc gaaggctaag cccgtccgtc ataaatacgg
    22261 agagtataaa aatgttcttt tgtcagatga gcagatggag aaactcaaaa tagaattccc
    22321 taatgactac caagagcgaa tagaacggct atctgagtat tgtgaatcat ctggtaagac
    22381 ttataaaaac tatttggcaa ctattcgaag ctgggcaaga aaagaaaaaa gtgaatctaa
    22441 gaacgcaagc agtggataca agcgcacagg aagacgagag aagcttcctg agtgggcaat
    22501 cgaccaagaa gcctatctca agaaaaaagc gctagaacga gctaatagac aatcaaaagc
    22561 accattctaa gaggtgggaa attgaaaatc gattatctag aactaattaa tgaaatagca
    22621 aagtataaaa ctggtgagga aatagaaatc ctgagagacg tatatgatca actcgaagaa
    22681 gctggaatcg aaggaattaa gaatgatcgt tcgagttgga gtaagctcag atactatttc
    22741 gcactttata tcgatacaac acaattaaga aatttagcat ataccaaatt actatttgtt
    22801 gattgtgtta aaggcttgca aaaacatctt aatgaacttg agcaggtgta atcagatgga
    22861 cctaaagaca tttacagcac agatcgaact aatgcatcaa gaagctttaa gacaaagcgc
    22921 ctcgtacgaa gacaagtggc tcaacacgtt ccatggtgga cgtgagagcg cacttgatca
    22981 agtactcaaa ttattgaaag gggaacgtcg ggatggataa gaaagcggca atgaaacgaa
    23041 tcatcgaact gacacattct gagaattggc aagaagacaa agaaatagtt acagaagtcc
    23101 aaaagctcgg taaatcaatg tggactgaaa agcccaaacg gagaacgccg agaaagattg
    23161 caatttggca tggtgatcga attctagtaa caggtactgc tgaacagtta tctgaaatta
    23221 ctggtctgag caaaaacatc atctgggata gagcaaggag cttatggatt gattcaaaag
    23281 gacgacaatt taggtatgtg gaggagaaaa aatgaacgaa ctaatcacaa aagtagagca
    23341 gtgggctaaa gacaagggac tggatcaagc agatccaaaa gcacagtttt tgaaagtagc
    23401 tgaggaattc ggggaaatcg cttcagcaat ggcaagaagt aatgatgagc tatttaaaga
    23461 tagcgtagga gacgtaatcg tcacgttgat tatcctttcc atgcaaaaag ggacaaacgt
    23521 acaagagtgt ttagaaatgg catacaacga aatcaaaggg cgcacaggaa aaatggtaga
    23581 tggtgtattc gtgaagtcga gtgatttgga ggacagcaaa tgataggttt aacaatattt
    23641 tggtgtgcga ttggtggttt actagcattt ctaggatact acctcatctg tgaaagtaga
    23701 aaaggaagaa aatatacagt gacaggtatt gtcctgttaa ttatcggaat tctagttgca
    23761 gttggagcag aactgaatct ttatgtaaat ggtacacaag aagatttggt gcgatttatg
    23821 ttttggatga agaattagga ggaaaaaaat gaacgaaaac aaattaatca aattaggtgt
    23881 agcaggagca gtaatcgtag gtattggagt tatcggagga tttaagttct tcgaaaaaat
    23941 cgataatgga tatgtgggtg tgcgctattc aatgaacggc ggtatcaaag atgaagcact
    24001 gacgcaaggt gtgaaatttg tagggattga caaagtgatc caatatccaa ttcgcttgca
    24061 aactatccaa tcaaaaaata tttcagtatc tacaagcgac ggcaaaaaga caacgattga
    24121 tatcaaatat gactacaaag ttgattcaac taaagcagca aaaatgtaca aggaatttgg
    24181 gaatatcact tcggaagata tcgaaagtgg atggttaaaa tctaagcttc aaaaggtcgc
    24241 tcgtgaagtt tatgcgaaat atagtctgct tgatgtcctt tcaggagatt cctctaaagt
    24301 tgaagctgag gtattaacga actttgctaa atcagttgaa tctaaagggt ttgaagtcga
    24361 agacgtaaca cttggtgttc cagatgtcga taaagaaaca caaaaatcaa tcgatgcgat
    24421 cattcgagct ggtcaagaaa atgaaaaagc gaagctagat gcagaaactg caaaaactca
    24481 agctgatagt gaagcttaca agaaaacaaa agctgcagaa gcagaggcag aatctaatcg
    24541 caaagtcgcc gaatcagtaa cagacaattt gattcgttat gaagaagctc aagctcgcaa
    24601 aaagcatgga tgggtaacag taaatggagc agatactgta gttacggatg aagcaggcaa
    24661 ataatatggg attctttatg gctaaaattc tcttgttctt aggtttagta ggagcagcat
    24721 atctcgtgta tgcgctcctt tcccaaactg atgacaaaga agatgacaac aacgatgaaa
    24781 tgaaatttta ggaggagaaa taatggatct cattacacaa tacagtgata tcatcctcaa
    24841 gaaaatcatg gcaaagattc agaaagataa aaaagcaaaa gaacgagcgg aattagttaa
    24901 gttggaaatg gctgaaacag gagtaggagt gcgaagtagc aggcattgga aagcagcagc
    24961 aaacattgaa ttttattaca acgaaattca aaaagggttc gatcagatgc gtgagctgga
    25021 tcggcaaaca aattggagca agaaacttca tcaagatcgt ttcaaatttg tagagaagta
    25081 taaagaaata ttagaagagt atttgaggag gacagcgaat gataaaaaag ctcattcaat
    25141 tcagcatgga tttatatgat atcgaatcag gagcaacact atctgtggaa tcggaccatc
    25201 taatcataaa ttttggtgga aagcgccaga ttattttgtg ggtagttgat gatgtactgt
    25261 ttccagaaat tgttcatgat ttcgaagaat caaaagcggt tgagtttgaa atagtgaaaa
    25321 aagtaatgga attgattgaa aaatacgagg aggacagcga atgataccga agtttagagc
    25381 gtgggataaa cgaaagaacg taatgagaga tgtagccgtc ttgcatttta ctaaaaacgg
    25441 caaaacaaac tttattgaat attggataaa tcctaccgaa ttgaaatcat atcacgtgcg
    25501 aaacatcgac ctcatgcaat ccacaggaat gaaagataag aatggtgtgg atatatttga
    25561 aggggatata gtattagtca gcgtgcgaaa tggcttcgat tacttagata ataaagtctg
    25621 tattgtcaaa aattcaatag attattccgg attagtttgt gccactgttg atgaagactt
    25681 agagtatcaa atttttaaca cagagctgtt tgaagaatac acgtacgaag tcaccggaaa
    25741 tatatacgag aatagcgagt tattggagga acagcgatga ataaacagga agcaatcaaa
    25801 aagttagaaa gtattaaagc gataggaaat gatgcaatag ctgcttgcta taacgagagt
    25861 ataaattcag gtattacgtt aatgaaaaaa atagacgaac cgcagaaacc agttattcca
    25921 caactcgtgg ccggttggct tgagaaatct acggaccctt ttacaaaagc tgaaaaaata
    25981 gcgtatttaa tcaaatctaa agatggtgat tcatattatt tctgtgattg gtttgtacga
    26041 gatggcatag tgacgcaaga gcaaggagaa gaattactcg cttgggcaac aagacaatca
    26101 tatgaaacac tattgagcct atacaacggc tacgaggttg agaaagagcc gttatgggca
    26161 ataaagaatg ccgatggaaa ctatcttact aaatgtgctt tatggggaaa agatggagta
    26221 aattatagtt ttgaatgcaa tccatctcat cgattgcttt tcactgataa agcaacagcg
    26281 gatgctgcag cattgttggt gaatggaaca gtggaagagg tggtagaaag atgaaactaa
    26341 aagacggatt ttacgctagc agtcacggta tcggcggttt aatgctagat atgccgacaa
    26401 agaaccctaa aacacgcaag aaatcaaaat tcaaagtcgg tgacatggtc cgctgtgaag
    26461 cagaagagtt cgtttatccg ttcagaggat acgttaaaaa gatactgtca aactcagcaa
    26521 tcattcgtat tgaaaataca atgaagtgtg atcgttcgac tgcaaaacac aagcattatt
    26581 tagctgtagc gagattagtt gacatggaag taatcaagag caaataaaaa aagccggatc
    26641 gctccgactg attcaataaa tccaacacat ttattatatc acataaagga gcggtttgac
    26701 ttgatgcaat tgttacgaga ggtagatttc aaacagacaa gatgtaatgc gagagatgtg
    26761 ctgaagaact ttcggcgttt ggagcggatg gcaggtcgct ctttgataga tattaagtcg
    26821 ccgattataa cggatatgcc gaaggcaccg aagcatggca ataaggcaga agacgcgatt
    26881 attcagatga tggatataga aacggagaga gatgcgattc tagcggcttt gatggctctt
    26941 agtctgatta gtcgtcagat actctactac agcttctgtg tgccagatag cttctcaaac
    27001 tacagaatta gccgtgaagt gggttattca gaaagaagta tacaacggat gaagtcggaa
    27061 gctctaatag agtttgcaga agcatataaa cacggaaaaa taattgctta taaataattt
    27121 ggcggttttt tggcggaatg atggcggttt ttagacattt accagtgtta ttatggtagt
    27181 gtcgaaagat taggaaacag gatcgacaaa ataaacgaag ggaggcaatc tccctcatcg
    27241 ttttaaactt cttcttagat agacagcaac taattatgaa gaaaaggatg tgaattcaac
    27301 tcctcctaaa ttcttcatcg gttgctgttt attttgatta gacagcagca ctcacaaaac
    27361 taaacatgtt cttgtcgtct ttcaactgct gctgtctatt aatttatgta ttggagggaa
    27421 gaaaaatgaa attatcagta gaaggcactc cagaagaaat acaagaattg ctccaagcta
    27481 ttggtagtag caaggagcaa aaggtaagtg tttctaattc agatattgac ggtatttgca
    27541 attgtttaaa cagagaaaaa atatctaatc cttactaaga gaaaatatgt tcgttaatga
    27601 atttccaatt ttcctctgta gctctacctt gataattttt ttgcagttca cagatgaata
    27661 aggaatcgtt attatcaagt actgtattta atttttcaca catttgattt ggattcaaat
    27721 tagttttaac taaccaaaaa gatttttgta gctttatata tgccccaaat tcttttatta
    27781 tttcaaatac tttatcatac ttttgccctg ggttattaag atcataacta atcatgtagg
    27841 gtttatacat gttgtcttgc acatccttgt tttatatttc agcggaccac tcgctgataa
    27901 ataaaattat acgcttagta tttattttca caatattaat ttatcgtaat aacttttgag
    27961 taaatagaac aaaaaacctg cactagtttc cgctagtaca ggcagtgact atatcggttg
    28021 ataatctagc atatattaaa aataattgca agaaaagaaa tttatagtgg tttataccaa
    28081 attatcaaag caatgttatt ttgttgctgt ctattgtttt ttaattattc acacgataac
    28141 taaaggtggg tgaagagaaa tgattccatt aataatttca atttttgcgc tctgtcttaa
    28201 tgtctatatg attggattta aaaatgggca aaataaaaaa tagtagcagc caagaataat
    28261 tttatagtgt cactgtggcg gaaagggtag acgctaagca tgtgtgctag gtcaatgctt
    28321 cggcaaccat gcaaggttcg attccttgcc agcgacatta aatgcctatg acggttacga
    28381 ctaccgaaaa aagatcgtta agaagctata cggtgctacg tacggcaatg tagtaagtgt
    28441 gctatctgta caccaccaag cttcggtcac tgtggcggaa gtagaagacg cagcggtaaa
    28501 tggcgagtag cctcgtgaga gcctggtaag ttctcgtgag tggtgcaatc ccactccagc
    28561 gactttaagc aaccgaggca tcggcggttt aaaaatatag gggtgcgcaa tttcgtacgc
    28621 gttttgtgca tcgtgcaagt tactattaca tattagatca ctctttgagt ggtcttttta
    28681 tttttgcaca aaggaggaaa caacaatgta tagaccacaa tacttagaac agaagtatga
    28741 agtaatcact gttcataaag gtaatggcga aaaagtatat gagtatagaa gaccaataaa
    28801 gagcgataca tataaacgaa aggaaaacaa tgaagttatt ccattgtatg gcaaaagaat
    28861 agctaagcat taaataagat tgcgaaagga gacggaacat gaccgaggaa ttctatagat
    28921 ggctattaca gttgacaaga gaagatcgtt tggttaagtt ctatcagtct cctaaatggc
    28981 gcaggcttag agagaaagcg atgaaacgag atcactatga atgccaagag tgtagaagac
    29041 taggtaagta tcatagagta gagaacgttc atcatataaa ggaagtcaag gatagacctg
    29101 acttagcttt agatttagat aatcttattt gtttatgtgt tgaacatcat aatgaagttc
    29161 atggcagata tcttacagca ttagataaac aagagaagaa gatagaaagc tttgctaact
    29221 tcgatgcaag tgaaaggtgg taagtgcatg atcatcaatg ataatggcag agagtatgat
    29281 acagaaaaga ttgaagagta ttcatcttat actcagggat taattaaacg tttgatatac
    29341 gttcgctatg taggtattag ggatctgtta tcagataact gttgtagtaa atacaaagtg
    29401 aatcaagtaa gagaagcgtt gaataaagat aataacgtcg aaagaataaa aaatgttttt
    29461 ggatatagta ttgaagagat taattattac attgacttcg ctgaagcttt cattccgatg
    29521 gtgagataac ccccccttaa aataaatcgc aaattttttg ggggtgatga aacggagggg
    29581 gctgtcagga aaagagattt tttcgaactt tatcatgaaa ggagggctaa aatgtttaaa
    29641 aacgaattgt ctcaaaatcg ctacagagaa aaattacgcc gctctttaat aagccaattg
    29701 gaaagtcaga aaacaaatat tgagccattc ttagataatg ttgatcgtta tatcagttta
    29761 tgggaaacgg cgatatcact ggaagaagat atatccgaga acggcattag actggagaat
    29821 ggtaaaaaga atgaatcagt agcgttgctt gtttctgtca acaaacaaat gggattgatg
    29881 ttggataaac ttgccattac tcctgaattg gtaggtgaag caaatgaatc aattcctgag
    29941 ttataagcat attgaaaatt ggttcaaagc tatagaagaa ggcactatca aggtatgcaa
    30001 agagcaatta ttgctaaaaa attatctaga agaaagagtc tttactagag aagatattta
    30061 cttcgataag cagatggtag aggattcaat caatatacca gcacaatact ttccattcga
    30121 attaattccg tgggaaaaat ttctacaatg ttttatttat ggtgttcgat ggaaaaaaga
    30181 taaaacacta gtgttcaata gatatctttc attaatggga cgtggtaatg gtaaaactgg
    30241 ttttgcttct tggaacaact tctttctact aaccgctaaa cacggtatta aaaattatga
    30301 tattgatatc tatgccaata atgaaagcca agcaaagact agttttgatg atgtatttaa
    30361 agtaattaaa gatcatcctg atttagataa aaaagtattt aaagctacga aggaagttat
    30421 tcaaaatatc gctacaaaca gcaaacttcg ttataacacg gcaaatgcta gaacaaaaga
    30481 tgggaagcga ccaggtgcaa accgctttga tgaaattcac gaaaatgaag attattcaat
    30541 gataaatgtg gctacttctg gtggtggtaa aattcgagat tatagagaat tttatgatac
    30601 aactaatggt catgttcgtg gtggtccgct tgatgacatt atagaagaat caaaaatgat
    30661 tctttctgga gaacttggaa ttgacaagga tggagcagaa ttttctagtt tgtttccatt
    30721 tatttgtcgc ttggataacg ataatgaagt tgatgatccc gacatgtggg aaaaagcttg
    30781 tccaactatt aattacaatg cagatttaaa acggaaaatg tttcaagaat actctcaaat
    30841 gcaacgtaat gctggtttaa gacttacgtt catgaccaaa cgaatgaaca gacctatgga
    30901 agatacacga tttgctgttg cttcatatga tgatgttctg catacgaaag aaaaagaatt
    30961 tcctgaaaaa atggatgaag tgataggaac agtcgatttt gctgatagac gagattttgc
    31021 cagcgttggg ttgctaggaa aatacgataa agatgtttat tttacacaac atacttttat
    31081 ccacgaatca gcccttcgat tacaaaacat caaacgagag gttatagata tttctataga
    31141 tcaaggaaaa tcacagatcg ttcatggaaa aaatatagaa gctgattata ttgtaggttg
    31201 gtttcttgaa atgagtaata aatattatat taaaaaaatc gctatggata tgtaccgtgc
    31261 aaaaatattg aagcccgctt tagaagaagc aggttttact gtggaaattg ttcgaagcgg
    31321 atctgttaca catggtatgt taaaagatct ggttgatgac ctttttatta atcaacgttt
    31381 attttttggt gacgatgcga ttatgcgttg gtattgcatg aatgtatatg aagagcatat
    31441 ttctaatgga aatatacgct atgaaaaaat agaacctgaa actagaaaaa cggatggctt
    31501 tttttcattc cttcatggtt tgaatttttt agatgatatt tatgattctg ctcctgtaac
    31561 agtcacaaat agctcagtag aaaatacagg aactggattt actcctctag tattctaact
    31621 tgaaaggagg tgagaaagtg gggatttttc aaaaggcggt aggatacttc acaaaaaaag
    31681 caacggttcc tttagaagaa tacttttgta aattgcaagt tgattttgtg tatcgaaaat
    31741 ttgcgattga aacttgtatt gatttgattg caaatgcgat gagtaaagcg gaattcaagt
    31801 catatgaaga tggaaaaaat aaaaagaatg atctttacta taggctgaat gtagctccta
    31861 ataagaaaaa taatgcaaca gaatttagaa aaaaactgat caggagatta atattctaca
    31921 atgaagtatt gatcgtttct ccgtctaata attctagcga aatatttatt gcggatagtt
    31981 gggatgtcac agaatatgca ttgaaagatg atgtgttttc tcaagtgcaa attaacaaca
    32041 tagtccttga tagagaattt ctagaaagtg atgttatcta tataaaatac gcagatcaac
    32101 aaattaggca actagtcgat gcgtattatc aagcgtatgg gaaactcatt tctagtgcca
    32161 tgaatgttta caagcgctct aacgctcgta gatacgtact gaaagggaat ttattccgac
    32221 cgcaagacaa tacaacacaa gatcaaatca ataaaatgat gacatcacaa tttaaggctt
    32281 ttatggaagc tgataatgca ggtgcggtat ttcaattaca aaatgagtac acattagaag
    32341 atttcagcgg aaacttccaa agcaattcaa gagatataaa aaacttaata gacgacatct
    32401 ttgagatgac agcagcagcg tttcacgttc cgaaaaacct actaaaggga gacatgagtg
    32461 ggttatcgga tcaagtggac gcttttttaa tgttcgaaat cataccgatt gctgaactta
    32521 ttcaggatgc gtttaacgct agtctctatg aagtagaaga atacttgtca gggaattttg
    32581 tacgtgtgga tacaactatg atcaagatta ctagcttcaa agatttggtt gacgctattg
    32641 atgtaggcat tagaaatgga gtatttacaa tcaacgaagg aagagaacgc gttggaaatg
    32701 atcgctctga taaggcgatg gcagatgaaa tatttataac taaaaacaat caacaagtat
    32761 cgaaaggagg tgaggcgaat gacgacaatg aaaacatttc tagcagtaaa gaatgaaggc
    32821 gcagtaccgc aaatttttat tcagggattt attggttcta gttggttctt tgaagggaat
    32881 actgacaagg gaatcaaaaa tattttggat agtctaggtg atcaagaaga aattgaagta
    32941 gtaattaatt caaacggtgg agacgtattt caagggattg ctattgggaa cttacttaag
    33001 tcaaataaag caaaagttaa cgttgtgatt aacggcttag ccgctagtgc tgcttcaatt
    33061 atcgcaatgg ctggcgatac tataaaaatc tacaacaatg cacaattgat gattcaccgc
    33121 gcttccacat acggagaagg aaatgtcgat gacttccgta cgattgctga ccaactggaa
    33181 tcaattgata aatcggtaaa ggcttcatat aaaacacgat tcaatggcac agatgaagca
    33241 ttgcaagaac ttcttgaaaa agaatcgttt atggatgcag aaacagcttt gagttatgga
    33301 ttggtcgatg aaattatcga tgcagaaaat agctcaggta ctgaagctaa aaaagaacaa
    33361 agcgttgaag aaattttgaa tgacgttaaa gaaaaaagag cagaaaaaat tgctgcattt
    33421 acagcagcat taaataaaac atttggacaa ggagatgtaa aataatgaca gttaaaaatt
    33481 taaaaggtgt aacagctgca agcgaccaat tgatgaaagc ttttaaagat ggtaacgaag
    33541 aatcttttag cgcagctatg gtaagcttat ctaaggaaat tcaggataaa attttagaag
    33601 aagcaacagc aaaaaatcaa gatcaattag tattaatgaa ccgtggtcag cgtgtattaa
    33661 ctacacaaga aacaaaattc tataacgaag tagtgaaaaa cgaaggtttt gcaggggtcg
    33721 aagaattagt gccagctact gtatttgaac gtgtatttga agatttagaa caatctcatc
    33781 cactattgca aaaaattact tttgttaaca caactggtgt aacagaatgg attgtgtcac
    33841 gtggagtcaa tccagcatgg tggggtaaac tttgcgaagc tgttaaaaaa gttttagata
    33901 atgactttga tgtaattaac atgaagcagt tcaagctatc aggttatatt cctgtatgta
    33961 aggcaatgct tgatttaggt ccagtatggt tagatcgtta tgtccgtact gttttagtag
    34021 aatcattgag aattgcatta gaacaagcaa ttgttgatgg tactggtaaa gatatgccag
    34081 tcggaatgat gcgtgacatg agcaaacaaa ctagcggaga atatgctgaa aaaaaagcag
    34141 aacctattac agctttagat gctgtaacta tgggcggttt gatggcgcga ctatcaaaat
    34201 tcaatatcga aggtgtgaat gatccgattt atcgtaatgt gaatccttct gatgtggtcc
    34261 taattgtgaa tccaactgat tactggtcta aagtattccc agctaagact gtactaactg
    34321 ctaatggaga atacgtacaa gtattgccag taccagtttc agatttgcag tcaacggctg
    34381 tgccagaagg aaaagcagtt attggggtag cctcagatta cttcatgggt gtaggatcta
    34441 cactaaaaat tgaagcttca gatgaatacc attttgttga agacgaacgc atttatctag
    34501 ctaaacaata tgcaaacggt caacctaaac gtaacgatag tttcattgta ttagatatta
    34561 gcgctttggg aactactact acaactacaa aaccaacaac cacaacaact acaacacaag
    34621 cgtaggtgat cagaatgaag tatattcttt gtcagccggc aatcaatcgg tttaaatggg
    34681 agtttgaagt ttgtttaact aatctgaaga aactaggaat caaagatatc gtattgcttt
    34741 tcagcagaca cgatgatcag attcctattt tttttgagaa ggaatatggt gttgaagttc
    34801 atgtgtacga tgatctgcgg gacgacaaag agtatattcc ttcgattaaa ccatatttat
    34861 ggtggaaata tttagaagaa gatcattcgc gtgaggacga ccgatatttc tatatcgatt
    34921 cggatgtcat tttcaataaa agaattaatt tgcgcaaatt gccttctaaa gatgatgttt
    34981 ggtattgtag cgactgctgt agttatctaa gtcttgatta tattagaagc tgtgaaaacg
    35041 gagaaaatat tctaaaagat atggcaaaca ttgtaaatgt tacagtagaa tctttggaaa
    35101 ctataaacac taattcagga ggcgcacagt gggttattaa ccgtcctaaa gcgaattatt
    35161 ggaaaaaggt ttatctggat tctaatcggc tatatcgcta ccttagaggg caaaaaacaa
    35221 atatacaaat ctggacagcc gagatgtggg cacagctttg gaacatgatg tatttcaata
    35281 ttggtcctaa agttcacgag gaattagact tttgttttgc tactgatcca atagaaaaag
    35341 ttaaagaagt aaaaatctta cacaatgctg gagtaacaac aaacgatgaa gatttatttt
    35401 tcaaagggag atacgtgact tctacgcctt ttgatgaaga tttatcattt gtaaacaaga
    35461 aaaaatgctc ttacgcatat gttaaagcaa ttaaggcggt ggttagatga cgcctgaaca
    35521 agtgactgaa gaattgctaa tagctgtgaa ggataatatt tacgttacct ggaacgaaga
    35581 agatgagtca attaaaaaga tgatagctaa aaatgctgtt tatcttcaaa gtaaagtgag
    35641 tacaacactt tctttttctc ctgaaagctt agaatacgga ttgctaatcg aaagatgtag
    35701 atacgactgg aatcgtgctt tagatgagtt tgaacaaaat ttcgctagtg agttattagg
    35761 tttcattcaa cattatgcgc tacaagaata tattgcaggt gatgtgaatg gcgaataatc
    35821 gtagactcga agaaacattc aacgatggtt ggttaaagat tttgacgcaa actaccaaaa
    35881 gaaatgaact aggaaaaaag attggtgtag aagatacaga aatcacttct ttaaaattta
    35941 gaaatctttc catgagagat agtgatataa cagctatgga tgcgatggga tcgaaattaa
    36001 ctaagaaagt aaagactcca tttcatccaa tcgccaagaa atttaataaa gatcaatatt
    36061 ttatcgtaat cgatagtatg cgttacaacg ttatctatgc cgattacgat aatttttata
    36121 tctattttta tcttgaaagt gtgggtgaat atggtgattg ataattctaa agaaaaagaa
    36181 cgtttaaata agcaaatttc tgctatcaaa acttccttag aagagcattt tggcctcaaa
    36241 ctctttcaag actccgtagg cgaggatgag ctacctgatg attttaatta cttcattctc
    36301 gaaacaggag aaatagaaat gatcactgag ccaaaatata gcgtgggtca aaatctatat
    36361 ctaactttct attcagaaaa tagagaagat ttaacaggag actcactaga tattatttca
    36421 ttgattcaaa atcgttcgat tcgttttcag agaatggatc ccaatcattt aaaactagag
    36481 aaccaagatc gctatatcga tcaattggta tttacgttta gacgattatt gaagagtgat
    36541 tgtcatggct aaaaatagtt gggagctaaa aataaatgga catgatgaac ttcttgtgcg
    36601 gatggaacgc tattcaagcg agagcgaacg actgattaac gaagcattga aatcaaaagg
    36661 ttcggctatt gcagtggata ggattacaga aaaaattcct gtttctgaag cagatttaag
    36721 aagagggcac caacacgcaa aaaatagtcg tccacttaag actcaataca ttaatttggg
    36781 tttcatcatt agacctacaa gaaaatttga gtatttaaaa tatcctgatt tggggatagg
    36841 tacttctaaa agaaatcagc cggacgaatt tatgagaaga ggattaggtc ttgcacttga
    36901 tccaattaca gaacttctga ttcgtcaatt cgataaatta aataaatagg gggaacaaca
    36961 atggctaaaa caaaaactgt agtaacaacg ttcgataacg taagtatcaa acgaattgct
    37021 tttaatttta agaacgcagg aaatgcaatc gcaacgaatt gtaacggaca attagatggc
    37081 gaaacagaaa tgcaaacggt ggttaaaaaa tgtggagcga cagaagtaaa atcaaaatct
    37141 aaaccaatca atatgacggt aacaattact gcacatgtac cgatggaagt ttatcgacgt
    37201 ttcaatgggt tgaaacaaga tggacgtatt aaaccaggca tttactctta cggtcctgat
    37261 tccgtaggcg aagatttctc acttgctgca gagatcgtgg atgacttcga agaaaataga
    37321 aagttagttg gtatgttagc atgcacttcg aatacaggat taacattctc tattgaaaat
    37381 ggtgcggatg aagtagctgc gttagaacta gaagcaaaag ttatgcaaga tgaatttggt
    37441 aaattttatc atgaagcaat tgttgcagaa cttgaagaag acttaacaga tcaatggatg
    37501 acgaatctat ctgctgatgt gattaaaaaa acttcagttg tggcaactac ggccactcaa
    37561 tcacagtaaa aaaaacggag gtagcgaaat gaacgaagat tgctcaaaaa ttgaactaaa
    37621 cgatggaaca attttgaatt tagaacctaa actgaatatc aagaaattat tgatgatcaa
    37681 tagagatttt aacacagacg agtttgcaaa aatgtcgatg ggaaaaggct ctatggatat
    37741 tacagttatt caaggtgcaa aagccgtata tgtagcttat cgtcaagcga acatggtcga
    37801 ttacatttca ttcgatgaat ttatagataa atgggatttt gatatggagg ttgcagtagc
    37861 tgtatacagt actatgatgt tcaaacaagc acgtgatgcc tatcaaaaag aattcgaaaa
    37921 agcaaataag gaaaaaaagc ttcaaaagta aaaatgccaa agctcttagt tgaaacgtgg
    37981 gtcgatgtct attcgatgtt gaccgacgtt ttttctatgc cttcagattt ggttttaagc
    38041 gatatctgtt tagatgacat tttgcaaatg gcttacaaca agagcgctta tgaaggatgg
    38101 aagaactatg caataaatca atcccagaaa aactaaagga aaggaggtaa aaaatggcta
    38161 aaaagagaac ggaagcagaa gtaactttca tagctaacga tgacggattg aaatctacgt
    38221 taaaagaaat cagtgctgaa ttaactaaaa atagagcaga attaaaacta gaacaagctc
    38281 aattacaaca gactggttct gaatcagaca agttaggaag taaattatct tctttagaaa
    38341 agcagtatga attacaaagt caaaaagttg aagtaactag tcaacgttta gccaatgcca
    38401 aaaaatatta tggagaaaat tccaccgaag ttcagaaact tgaaagagaa ctgattaatc
    38461 aacaaacagc tcagcaaagg ttatctaatg atatagataa aacgagtaag gcattggctc
    38521 aatctaaagg tgaatttaaa acgtatgagt caactatgaa agacttagat aatgagcaac
    38581 aacatctaaa gtcaagcgca tcattagtag aatccgaata taaaaaatgg caagcaactg
    38641 ctggccagtc tgctacagaa tctgaaaaat tagcaaaagc acaagaatat gtcggcaaac
    38701 agagtgatat tgctgaacaa aaaatcgaag tgctaaaaaa acaattagat gccacacaaa
    38761 aagaatttgg agaaacatct accgaagctt tagaaatgaa aactaagctt aatgatgcgg
    38821 aaagagaatt cgaagaatta agtaatgctg ctaaaaacgt tgatacctcc acagtagatg
    38881 atatcggcaa aaaacttgat atgggtaatt taatggaagc tactgatcac ttgtcagtga
    38941 tcggagataa gcttattgat gtaggtagta agtctattga agcagctgga aaagcacaag
    39001 ctatgcaggc ccaatttaaa caagtctttg gctctttaga aggggaagca caggacgccg
    39061 ttgagggaat ggctgaagaa tttggaatgt taccaaatac gatcaagcct gtttttacac
    39121 aatatacgtc aatgtttaaa ggacttggat atgataccaa agaagctatg gagttagctg
    39181 gtgatagtac tcagttagca gcagatgcag cagcttttta cgataagtct atggatgatg
    39241 ctagtgaatc tcttaattca tttataaaag ggaactacga aggtggggag caaataggtt
    39301 tatttgctaa tgatactcaa atggcagctt atgctgttaa gcataatttg ataccagcga
    39361 ctgaaggagc aaaaaaagcc agtgaagagt cattgttagc tgttgaaaaa gcacaatcta
    39421 agtatgctga tgctattaag aaacatggtg aaggatcttt agaagcaaga gaggctgctt
    39481 taaaacttaa agatgcgcaa gataaaataa atgaagaatt aggcccacaa acgcaaaaat
    39541 ggtctgattt agatgaggct accaagcaag cagttcgagt tcaatatgct gaagatatgc
    39601 aaaaattagc cggtgctaca ggtcaggcta gtagggagtc tgatggttta gaaaaccaaa
    39661 tgacaagagc aaagcaagcg ttagaagatt tttatgcctc attgggtgaa gatatactgc
    39721 ctgtgtttat caaagggttg caagcaggag cgaaagcttt gcaaggatta gccgaatggt
    39781 ggagtaaact tgatgggcca atgaaaaatt tcattttagc tcttggagga attctagcat
    39841 tattaagcac attagctcct gttataaccg cagttgttac gatagttggc acatttggtt
    39901 ctacagtttt gctgccaata ataggaatta tagcgggcgt tgcagctgta atagcgattg
    39961 taattacagc gtttcaaaac tggggcgcaa tcactgactg gtttagtgat ttatggaaaa
    40021 aatttaccga ttggttaggt gatacctggg aaagtataaa agaaagtgcc tcatcagttt
    40081 gggatggagt caaagaaacc tggtctggat ttgtagattg ggttcaagaa atttggcaag
    40141 gagtttctga ttggtttgga gagttatgga gcggattagt tgaaggagct tccaacatct
    40201 ggcaaggagt ccaagagact tggcaagcat tcgttgattg ggtttcaaat atttggaacg
    40261 gagtcaaaga agtatggtcg attatttggg cagacattgt aggaattgtt caaataccat
    40321 ggacattaat aacgtcattg attcaagctg gtattaatat tatcgtgggt atttttgatg
    40381 tagctggaca gttattaggc gcagcttggc aagctgtttg gacacctatt tctgatttcc
    40441 ttaaaaatac ttgggatact atgacacaat ggataagcat cgcttggaat ggaattgtaa
    40501 ctacattcca tactatattt gatccagtag tggcatggtg gaatggtata tggacatcta
    40561 ttagtactac ggcttcaaat atttggaact caattagtgc aacagcttct agtatttgga
    40621 atagtatcaa gaatacaatc actagcttgg tacaagcagc tgctacagta attcaaaata
    40681 tttggtcaac tgtatctagt tggttaggtg gaatttggaa ttcaatcagc tctacagcat
    40741 caaatatctg gagtagcgtg actagcagta taagcaatgc tataaacgca gctaaaagtg
    40801 ccattcaaag tgtttggaat agtatatctt cgtggatcag cggaatttgg aacggtatca
    40861 aaaacactgc tttgaatctt tggaatggaa ttacaagcac tattagctct aaagtaaacg
    40921 atggaaaaaa tgcaatttca agcggttggt ccaatctaac aggtattgtt tccgacatat
    40981 tcaataatgt taaaagcacg atagctaata tatgggaagg tatcaaaaag actgttagcg
    41041 ctccgattga ttggattaga gataaaatca gtagtgtctt tgataatttg aatatttcga
    41101 taccacatat tccgttacca cattttaaat tgagcggaga attcaatcca ttgaagggga
    41161 aaatcccaac attgggtgtt gattggtatg cgaaaggtag tgtgtttaat tctccaaata
    41221 ttatcggtgt cggtgaagca ggacctgaag cagttttacc tttgaaaaga tctgtgctgc
    41281 aagaaattgg ggatcgtatc ttgagtagca catcagtttc atctagggca caaacgattc
    41341 aacctgtgaa taactacgaa ttcaatctca caattgatgg taacgcagat gaggttacta
    41401 tgaagcaaac aactcaacaa atcattgata gcattacaaa agttcaaaat gataatgctt
    41461 cggcatggcg ttaaacagga gagtatttct cctgtttttt tagttttaaa aaggatgtga
    41521 aaaaatgact gattgtatac attctataat cgatggattt cctgattatt tgcataaatt
    41581 ggctttagcg gaaagaccaa ccataccttc tccaaaaaga cagagagttg aaacttctgt
    41641 tttaggacgg ctaggtggct tagtacaaga ttactcgttt gaagacatgt cgtttacatt
    41701 gcactataac tatttagaag atgtggaaga ccatcaagca ttcaagcaat cgttttatat
    41761 catgcgtcat tggttaaact atgcaaagaa attagaattc tctgatgatc ccaacgtcta
    41821 ttatgttatc cagactatcg atattgggga tgcagaaaac gatattgttg aatggggaga
    41881 gttcgatgta aatatcactg cgaaaccatt cgcaagagtt caagaagatg tacctataac
    41941 cgtagataaa ccacagtcat ttaacttgct gaataatagt ttagaagaaa gttttccaaa
    42001 gattatcatc actccttcag ctacttcatg ccaattcacc ttaaatgatt atgtgtttag
    42061 ttttgaaggc ttagtagtgg gaactgacat agtcattgat agtgatttga tgctttgcta
    42121 cgaagagcaa tcggacggag atattttaga tcggtccaac aaaatgaaga ccatgcaata
    42181 tccaacgttg caagtggata ttaatcattt taattgtact ggtttgagca aaatacaaat
    42241 ttatcgtaat gggttaaggt aggtgaaata gatgatcgat aatttactaa ctatttacga
    42301 taaaaatgac gcgaataatt tagctgaaca tttatatgat acgcaaggtt taggcgcttt
    42361 gtcagactgg ttaacagcta ctgttagcaa taaactaaac ggagccgaga tatttcaggg
    42421 tacttattca ataaacggaa ctaatgcaga tttgattgta gaaggacgta ttattcagtg
    42481 ttatgtagat gaaaatcgag caaaacagcg tctacggatt tattatgcaa aaacttctgt
    42541 aataggcaat acgatagaag taaaagctga acctattttc aatgatataa gaaaatcggt
    42601 gttgaataaa tatgacagcg gaacagaaaa aatcactgct actcaggcat ggcaaaacgc
    42661 aaaagtttta gcgaaaccag ctatcccttc gcagttttct ttctcgtcat tagtagatac
    42721 gcttgctaat gtgaagatag aaaaagcgaa ttttttagaa ttctttggtg gaaaagaggg
    42781 atctattcta gatcgatttc atggggaatt tcttaaagat aataacacat tacgtcatga
    42841 aaaaaggcta ggtacggatc ataaaatcaa agcgatttat actaaaaatt taactggtct
    42901 tgacttagag atagatgctc aaagcgtttt agttggagtt tatccattca ttagcagttc
    42961 ttcagaagga gaagacgaga tcactctacc agaagaagtc attttcacgg attacgtgga
    43021 tgattatcct gctggatatg tatcttttgt tgatttcaaa gacaaagcga ctgatgtagc
    43081 cacattaagg gaagctgcta aagactggtt gaaaacaaat atagataaac aaaaaccaca
    43141 agtgagtggt tcgattgaat tagtaccatt aaggcatcag agaggctatg aaaaatttgt
    43201 tgatttagaa aaagtttcga tgggtgacgg agtagatgtg tatcatccac agttaaaagt
    43261 gaatatgtca gcaagaatcg tggaatatac gtttaatgtt ttaaccaact catacgataa
    43321 attagttgtt ggaaacgtca aaacaaactt cttagaaaat acagagaata atgtaagcaa
    43381 tttgattaat gatgccattg atcaattgaa aaatggtggc gaaatcagtg atttaatcaa
    43441 tgatattgta gatcatcaaa ctgatatgat tactgggcaa aatgggggtt atgttttatt
    43501 agatcctaaa gaagcgccta gtcgtatttt gattatggac acgccagata agaataccgc
    43561 acggaatgtt ttacaaatca acaacgctgg tattggtttt tctaaaactg gtattaatgg
    43621 aacatatgaa accgcatgga cgttagatgg cggattcaat gcctcgttta ttacagctgg
    43681 tgagatagta gggattacta ttagaggtac tacattaatt agtgatggtg ctgattatag
    43741 aacaagtata gctaatggca aaatgacttg gtactcaaaa aaagttaaca aagatattat
    43801 ggagctagaa gcacgtgatt atgtaagtgc tgatgccggc attgtatcat acaccatgaa
    43861 aactggtggt ggtttcatga ttagaaatcc acaggggaat ttggttttta gtacgtggga
    43921 taatggtaat aacagaccgt ttttatcttt tggtgcgcca aatttcagat atagcaatgc
    43981 tagttatgta acttctggcg atggtaattc tttgggtata gatggtagtg cgggtaactc
    44041 atgggaattt aaggtagctg gtaggactat gaaatttact agtgatggta tgctaacgtt
    44101 accaggttgt ttttttggtt catgggaaga tgggaaactt gctaggtttg aacaatcaac
    44161 ggtacaagta tataaagatt ttactgttag aggtactaaa aactcaactg taccaacaga
    44221 acattatgga caacgactat tgaacgctta tgaaactcca gaatattatt tcgctgatta
    44281 tggggaagcc gttacaggtg acgatggtaa agttcgtgtt gatattgacc ccatgtttgc
    44341 tgagacagta aatctaagtc ggtatatgac acatgtgaca cctacagaac tagttttgtg
    44401 tgctgttact catgaagata ttgaccattt catcattgaa actagtaagc caaacgtatt
    44461 agttagatgg aatttagtgg cacaccgtct agggtatgaa gatattagat taaaagagga
    44521 tacagcatat gatagcacag tgcttgacca aaaacgtttt taaaacgaag acaaggaggt
    44581 atataaatgg ctagcagttt atataatttg gctttagatt tcagcaaaga attaaactac
    44641 accaaagcta ttatggctcg tcaaggtgat aaagggatta cggtgactgt taaaccattt
    44701 ttaaatggct tgcagatgga tacgagtggc ggaacattta ctttaaaagg aacaacacca
    44761 tctaaccgtt atgtagatag tgttgcaact agcgtaacta gtgaagaagt catattttct
    44821 cttgatggca catttatgag tgaagcagga tattataaac actgctacgt agaatataga
    44881 aaaggtaatc aaattttaac aacgcaagac atcatttttt tctcactagg agtgtctgac
    44941 atttcgcaag gccaagccga tgaatacgtt tcgcaattgg aagagttgat tcgaaagtat
    45001 aacgaaactt ttgatgcttt tatggctgaa attaaaggta gagtggatag cttaaatcaa
    45061 cagattactg atttaactgg tcaagctaaa acgctacaag acaagttaga tgctctgaaa
    45121 gaagaaattt ctaaattagg taacttgcaa gtcatgtaca gtaacagcat cgacttcggg
    45181 ggctatgatt atagcgggaa tccgaattta atggctaata taaacgctga cagcttctcg
    45241 caaggtactg gtgctttatc tgttgtggat gatggtgacg aggtagtggt cacgcttgat
    45301 ccaaaccata aattagagtt attcaaagcg aaaagtcaac caaacctgtt aataggtaaa
    45361 acatatacca tgagtgtaga aattatgtta gaagatgatt tcactggaga ccctagtaag
    45421 ataggtctaa gatatactaa aatgcctaac tgggtatcag aactatatac gcgtaataca
    45481 ttaactactg ctaagggtgt atggcaaaaa ctaactggca ccgttaaaat tactgctacg
    45541 agtgataacg ctgaaagctg gtttataatg ctacaaaata aagacgctaa taacagccta
    45601 tctggcaaac tacgtttgag acacgctaaa gttgaatacg gctcaacagc cacaccatat
    45661 caaccaaatt tattcgatgc accgtattat ttgagtaagg cggctttggg tgagaatatt
    45721 gctaataagt ctgtgacgtt tccaattaaa tctagtgatt atgccgtgta cgactacaaa
    45781 aacattgaaa attttgtagt gggggaaaaa tatgttgcga ctataaaagc aacaaaacct
    45841 gcaacacaag tatttgaagt aagaaataca aatagttatg gttttggtgc tatgaaacca
    45901 gtagaaggtt taactgatat atgggtatta gagtttactc caacaagaat tgatgctgct
    45961 agtccatcaa gtcttcagat atatcagtca ccaaagaata ctaaaggagc tgttcaaatc
    46021 gactggctca agatcgaaaa aggcgacaca cgaaccccga atattgagca atataaatac
    46081 cgaggaatcg gcatgcgaga ctcaaataat ccaaaagact atgtctggga tttagcacca
    46141 gaatatgtag aaaacaatct ggctacagat atcaaagttg ctgaaattac tggtaaagca
    46201 aacaattata ccgatgggaa agtatcggag attaattcgc agttgactgc ttcaattaat
    46261 gaagtagaca ccacagctaa ggatgctcaa acaaaagtga atgaattaga caataagatc
    46321 gatgaacgca ttaatgatac agctactacc gcattttatg gcgcacaagg tgaagtctct
    46381 aacaaaacga aagtagcgga gtatggagta ggaacagggt atactactac agctgtagga
    46441 gaaacacatt tcgaacgtca atctaacgga tacgttaaat gcttaaaagc tggtaagtat
    46501 tcaattagtg cgcaaatacg tgtgcaattg ggcgggaaat atgcttcatg gttatatact
    46561 gatttatata aagatggaac ttctgttgat aatttagtag cttatggggt cgacgccttg
    46621 caaaatagat ttgcagctag cggaaacatt gtaactgatt tagaagtaaa cgatgtacta
    46681 aatatgcgta ctgaaatcgg cgttgcagat ggaattttac aatttacatc ttgccgtact
    46741 ttagtcttga ctagactatc tgattaatac aatatttata attttgctat caacacgctc
    46801 aaaggagggt gttttttatt ttgcaatgaa aggaggctag ttggttgaaa gacgaagcaa
    46861 tacaagacgt ggtagaacgc ttagtgcgta ttgaaacgaa actggataat tacgaattat
    46921 tacgtgaaaa agcggaaagt gcaaaagata gagcggatca ggcatattct attgcgctta
    46981 ataatgcaga agacatcaaa gagatgaaag ccaataataa atggtcgtgg ggttacatga
    47041 tcggtttagg cattacaatc attggctatt tcttgactaa attgtaaagg aggtgagaag
    47101 aaatggtttt accagataag tattatcaag tcattaaatg gacagtttta acagtattgc
    47161 cagctgcatc tgtattagta gcgacgttag ggaaagcgta tggatggaat ggaacagata
    47221 tgacagtact cactatcaat gcagtagcaa catttttagg tgttatcact ggtgtgtcgg
    47281 cttataattt gaaaaaatag gaggaaacaa atgaaaaaga aaattactat tactgcgatg
    47341 agcctattaa cggctctttt tttattgcca attaatgggt ttgcctatac tatcaacaat
    47401 gaatttaatt tgggcgcaaa tgaaggtagc tcacaagtag caaataatca gtacatttta
    47461 ctgcatgaaa cggctaatga aacagcaaca ggacgcaatg aagcgcagta tatgcaacgt
    47521 tcatggacta gtgcttacac tgcttacatt gtgggtgatg gcggaattgt ttaccaagtt
    47581 ggacaacctg gttatgtaca gtacggtgct ggttcgtatg ctaatgccaa cagtcctgtg
    47641 cagattgagt tacaacacac acataataaa gcaacttttg aaaagaacta taaagcatac
    47701 gttgaattgg cgagagattc cgctaagaaa tacggtattc cgcttacatt ggacacgcct
    47761 tataaccaac caggaatcaa atcacattta tgggtaacac aaaacatctg gggcgatcat
    47821 acagatcctt acggttatct ttctgaaatg ggcgtaagta aagaaaaatt agcatatgat
    47881 ttagctcatg gatttaccga tgaaaatcca acaacttctg aaaacaagcc tgtcattgat
    47941 ccaacacgag ctggtgcagc taatcctaca ctgacagatg gaacgaatta cgcccacatt
    48001 gatcagtttg gagaaatcga aaatgcaaat ttgcatgtag ctggatggca cattgctaac
    48061 tataaatacg agtatatctt cattatggat tacaatactg ggaaagaatt agctcgagta
    48121 agagctgatg gaatttatag atcagatgta aatcaagctt ataatacttc tggaaatgtt
    48181 ggttatcatg tatctttcaa tatgcgtaat tttcctagta agaaagtcta tgtaatgatg
    48241 cgggcaacga atgatccaga gggaaacact aaaggcggtg cgcaagattt ccatgacaaa
    48301 cgttggtatt taaatattcc taaacgataa aaatagctcc tcgttgagga gcattacata
    48361 actatattga caactataaa aattattcga taaaatagtg atgttgtcac atatcttcac
    48421 tatcactcat aaatagtcac actccaagat atgcgataac aggtttgttg ccacaccttc
    48481 tactggttga ttgtttatgg ctttatgtgg caacaacctg tactcttagc tcagttggtc
    48541 agagcagacg gctcataacc gtccggtcgt aggttcgagt cctacagggt acataagaaa
    48601 tgttcaatat aaaggtctaa caaggaggat attggtttga gcttaaaaga tgataaacct
    48661 atacttagat ttaatgaaca actagaaaag ctaaatgatt taggaatcta tgtagcagat
    48721 aatgaaattc atgaagcaaa aaacattttg agtaatcaca attatttttt caagctatta
    48781 gcttatagaa aaaattatga taaaaatgag caaaaaaaat ataatatttc ttttttaagt
    48841 atagtagatt tggctaaact agatatgcaa cttcgttatg tgttgttacc ttattgtttg
    48901 gatattgaac actctttaaa aacatatctt atgagattaa ttactaatcg aaaaaataag
    48961 aacggtgaat atgcagaaaa tggttatgaa attgtaaaaa gtgttttagc agatgaagat
    49021 actccaaaag atttgaaaga tcaaatattt aatggagtga gatatagaga ttctgacggt
    49081 aatcttgctt ttcaagaagg atttgaaaaa tattataatg aacctcctat ctgggtagtt
    49141 ctagatttag cttctattag taaaattaag ttttttgtaa attatttggc aaataaacag
    49201 cctaataata ataatctaaa aatgattaag aacaatatag gatacgtatc gtacattaga
    49261 aattcttgtg ctcataataa acctatatta ttgaatttac agcggaaaag cataataaaa
    49321 aattctgtat atgctaatgc tagacggatt ggtattacac atgatgagat tacaaatttg
    49381 aatgttgcaa ggatatttgc tttgtttgaa ctacatagaa ttatgtgttc agaagggatg
    49441 cgttcacaca gaaataaaga tttttctaac tatttagaaa gagtaaataa aactttagaa
    49501 ctacatgaac aaaatatgca cattaagaat ttttttaatt cattaacaaa aattcttgac
    49561 gaattctgtg ttgaatgata gtattaattc aacttatgag gtagaaacct cgcttcgcct
    49621 cctttttagg ttggtgtagt aatatgaaaa aagaggagat attttttctc ctctttttat
    49681 tatttatata gggtcatata agaatggata atatttttat tgtagataga gggggcaaaa
    49741 aaggggcaaa aaactcttac tagtccgttc tagcctaata attttaagta ctatcaatgt
    49801 tgtttttcct tgatataatg ggatttttgc ccagtacgta caagctaaaa acgtttgtct
    49861 tgatgggcgg tatgatgtaa gaaaaagcaa cttgcttatc tacaactgat cattagtgaa
    49921 agacagataa aaagaggctg gaacagaagc gtttaactcc aagaaataag aagaaattca
    49981 cggaaattgc ttttcaaatt tttgtgaatt tcagcttatt tccgaaggag ttgcttctgc
    50041 ttccgctgtt tatacgtgtt tagagcgtga gacaaaagtg ttctttactt ttgtcccacg
    50101 ctcttttttg tgtgtaatta taatgaaaat agttattttt aatagttata atattttatc
    50161 tattaaaagt aaatatccat tc
//
LOCUS       DACGLE010000012        49865 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0534
            SAMEA3304449-rid9560453.denovo.012, whole genome shotgun sequence.
ACCESSION   DACGLE010000012 DACGLE010000000
VERSION     DACGLE010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304449
            Sequence Read Archive: ERR985064
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 49865)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49865)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702225.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702225.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 20:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,045
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 27 of 67
            Pseudo Genes (incomplete)         :: 25 of 67
            Pseudo Genes (internal stop)      :: 19 of 67
            Pseudo Genes (multiple problems)  :: 4 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49865
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304449-rid9560453.denovo.012"
                     /strain="VREN0534"
                     /db_xref="taxon:1352"
     gene            complement(87..266)
                     /gene="rpmF"
                     /locus_tag="IXT20_000823"
     CDS             complement(87..266)
                     /gene="rpmF"
                     /locus_tag="IXT20_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HAQ4859290.1"
                     /translation="MAVPARRTSKAKKAKRRTHYKLTIKGLNECPNCGEMKKSHHVCA
                     NCGYYDGKDVMSKEA"
     gene            complement(324..893)
                     /locus_tag="IXT20_000824"
     CDS             complement(324..893)
                     /locus_tag="IXT20_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HAQ4859291.1"
                     /translation="MKWSLLELRKYQETPLTFHETLDLKAALMKRDNLILDVAPVEVE
                     GLVSVDKKGYIVHYTVQTTLTVPSTRSLEPVELPMDFSVDEVFMTEEQFQSRDEQMSA
                     DEILLIEGPLLNLAESIEDNILLAIPMRVLTKEEEAATELPKGNDWEVVSEEEYQRRK
                     MEEAENKIDPRLAKLSELFDSKSKDDDNK"
     gene            1079..1981
                     /locus_tag="IXT20_000825"
     CDS             1079..1981
                     /locus_tag="IXT20_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAQ4859292.1"
                     /translation="MNTKDIEYYVQIVKQKNFTKVANYFRVSQPTITYALQRLEKEFD
                     TVLITRDRSHHELIVTPSGKQLFLHAQTILQEISTARAEITQLQKKKLRFGMPPIIGN
                     EYFPKLSSYFIKHELMNQIEITDGGSRDLYGMIRQGRIDLAVLGSTQPIVDQYLETEL
                     LLDKRFMIVVPPTHPLSKQDTVSFKDIADEQFVLLNEHYVHPAAFTKLSKQTHTHPKV
                     VYQSNDLTILKSMVRENVGIGFLAEIAVHPEDHLVCLPLSDEVQPRFLISLAKRTQQI
                     VTPLQEQVVQVIHEFVTAQKNSSS"
     gene            complement(2016..3800)
                     /gene="pyk"
                     /locus_tag="IXT20_000826"
     CDS             complement(2016..3800)
                     /gene="pyk"
                     /locus_tag="IXT20_000826"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HAQ4859293.1"
                     /translation="MKKTKIVSTLGPASNSVEIISQLIESGANVFRFNFSHGDHEEQL
                     SRMTMVREAVKKTGKDVGILLDTKGAEIRTTVQGTTEADFGRAGYIKFEVGDQTRISM
                     DPEHVGSKEKIAVTYPGLFDDVHVGGHILFDDGLIDMQIIEKDEANRELVVEVKNAGM
                     LGSRKGVNAPGVSISLPGITPKDADDIRFGLDNDIDFIAASFVRKAQDVLDIREILEE
                     KDMTHVQIFPKIESQEGIDNIDEIIKVSDGVMIARGDMGVEIPAELVPMVQKRIIKKC
                     NAAGIPVITATQMLESMQENPRPTRAEASDVANAVFDGTDATMLSGESANGDYPVEAV
                     STMARIDQEAENALSELGTFQLNEFDKTDVTETIGLSVARAAKNLGVKTIVAATESGY
                     TAKMISKYRPDADILAVTFDERTKRGLMLNWGVYPTVAEKPSTTDEMFELAAKKAVEL
                     GFASEGDLILITAGVPVGERGTTNVMKIQMIGSKLLEAQGVGQKSVVANAVIASSAEE
                     AVKKAKDGMVLVVPSTDKEYMPAIEKAAAVIVEEGGLTSHAAVVGIAQDIPVIVGAKG
                     ATTTITEGELITVDSRRGIVYRGETTAI"
     gene            complement(3874..4836)
                     /gene="pfkA"
                     /locus_tag="IXT20_000827"
     CDS             complement(3874..4836)
                     /gene="pfkA"
                     /locus_tag="IXT20_000827"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HAQ4859294.1"
                     /translation="MKRIGILTSGGDAPGMNAAVRAVVRKGIYEGLEVYGINYGFAGL
                     VAGDIRRLDVADVGDKIQRGGTFLYSARYPEFATEEGQLKGIEQLKKFGIEGLVVIGG
                     DGSYHGAMALTKRGFPAVGIPGTIDNDIPGTDFTIGFDTAINTVLESIDRIRDTATSH
                     VRTFVIEVMGRNAGDIALWSGVAGGADEIIIPEHDFDMASVAKKIQEGRDRGKKHCLI
                     ILAEGVMGGNEFAEKLSEFGDYHTRVSILGHVVRGGAPSARDRVLASKFGAYAVELLQ
                     EGKGGLCVGIHDNEIVASDIIDTLENNKHKPDLSLYDLNNSLSF"
     gene            complement(5004..8318)
                     /gene="dnaE"
                     /locus_tag="IXT20_000828"
     CDS             complement(5004..8318)
                     /gene="dnaE"
                     /locus_tag="IXT20_000828"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HAQ4859295.1"
                     /translation="MFFPQLYTITSYSLLQSTIRIREYVQEAKKQGYTSLAITDKDVL
                     YGAPEFYRICVEEGVQPIIGLHLSYTYEEKNYGLLAYAKDEIGYQQLMKLSSRKMIQE
                     KLELAEMTETDHLAFVVPANHSVSEAFNKEDNFFGHWQFLLENVFTRNFYIGLTENTP
                     DQLPEWYQYVIKENYSLCALHPIASLQQEELFALEVMKHIKEGSQIPLENLSASLPLT
                     ANVYLLSEQEMIKRFEKAGLSQAVIGAQRLAESISFEFQFHQKLLPHYPVPNGKQAGE
                     YLRELCYSQVERRITDYTKEYQERLDYELSVIHQMGFDDYFLIVWDVMAFAHRNKIVT
                     GAGRGSAAGSLVAYVLEITDVDPLEYDLLFERFLNPERFTMPDIDLDIPDNRREEVLN
                     YVREKYGQYHMAQIATFGTMAAKMVLRDAARVFGLSQSEANRWSKAIPNQLKITLTQA
                     YKTSHQLVELVQASEKNQLLFKTAVILEGLPRHVSTHAAGVVISDKNLLELVPLQRGS
                     DEAFLTQYTMNEVEAIGLLKMDFLGLRNLSIIDFTLKAVKRVEGYEIRLKEIPLNDAK
                     TLLLFQRGETSGVFQFESAGIRNVLRRLDPENIEDIAAVNALYRPGPMQNIDTFIARK
                     KGKEAIRYPDDSLIPILKNTYGVIVYQEQIMQIASTMAGFTLGQSDILRRAVSKKKKD
                     VLDEEKSHFVSGALQQGYSEKTAATVYEYIEKFANYGFNRSHAFAYSFVGFQMAYLKA
                     HHPGAFFTSLMNSVRHNTSKLKEYIAEARKNKLKLSSPSINQSNSSFELVNKEEIRFG
                     LTAIKGIRRDFVEDILNERKQNGPFQSVDQFLIRIDKRWLKLELLQSLTAVGVFDELS
                     PNRKQLMLDLEGKIQNIVYSGGSLDLLGIMALKEEEVADYTLEERLKLEEEYLGIYLS
                     GHPAEGFDRLRLAKQVQLITDIVPNQPVNLLVMIKEVREIRTKKGELMAFVEGTDSSG
                     EISVTVFPTLYRKYRNLFAVDRVIFVQGRSEISKYNQELQLIGESFADPNQLEAQYPS
                     QTCYLRITEEREDPEIMKQLQVIFQKHPGYIPVVMYHEKTQRKVVLSDAYWVDDSKSI
                     KGQLIYLLGEDNVVFK"
     gene            8549..8734
                     /locus_tag="IXT20_000829"
     CDS             8549..8734
                     /locus_tag="IXT20_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjzD family protein"
                     /protein_id="HAQ4859296.1"
                     /translation="MRYIVTLFWAVVLGQVVGYIGAALTSGTYDFTLTTIISFIAGVI
                     ILLIGAVAPRKETSAHS"
     gene            complement(8796..9992)
                     /locus_tag="IXT20_000830"
     CDS             complement(8796..9992)
                     /locus_tag="IXT20_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HAQ4859297.1"
                     /translation="MEINWRKNLVIAWIGCFFTGASISLVMPFIPVYVEQLGTPKDQI
                     ELFSGLAISVTAFASAVVAPIWGNLADRKGRKLMMIRAAAGMTLTMGSLAFVPNAYWL
                     LIMRFWNGILSGYIPNATAMIASQAPREKSGWALGTLSTGAIAGNLIGPSMGGALAQW
                     FGMENVFLITGALLMITTVLTIFLVKEDFHPIEKKDLISTKEIFSKMDHLSILIGLFI
                     TTLILQIGITTISPILTLYIRELSGSTENILFVSGLIVSIAGVSAVFSSPKLGKLGDK
                     IGNQKVLIGGLILSFCCYLPMAFVTTPLQLGILRFILGFSTGALMPSVNTLISKITPQ
                     EGVSRIYSYNQMFSNFGQVLGPMLGSTVAHAYNYSAVFIVTSLFVLSNILLSLFNFRK
                     VLRSRL"
     gene            complement(10162..11598)
                     /locus_tag="IXT20_000831"
     CDS             complement(10162..11598)
                     /locus_tag="IXT20_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HAQ4859298.1"
                     /translation="MEKFFGLKKNNTNVSTEIMAGVTTFFAMSYILFVNPTILSASGM
                     PFQAVFLATIIASIIGTLVMGLFANVPYAQAPGMGLNAFFTFTVVFGLGYSWQQALAM
                     VFICGLINIFITVTNIRKMIIRAIPESLQHAIGGGIGIFVAYVGIKNAGFLSFSADQS
                     AISSSVVEGGKATNVTINGGIVPALANFDNAPILLAVIGLVLTAILVVKNVRGAILIG
                     IVATTVLGIFMGVVDLSSIDWQTNSLGNSFKELGTTFGAAFGSEGMQSLFSDSSKIPQ
                     VLMTIIAFSLSDTFDTIGTFIGTGRRTGIFSKEDEIALEDGRGFKTKMDKALFADAIA
                     TSVGAIFGTSNTTTYVESAAGIGAGGRTGLTSVVVAVLFALSSLFSPLIAIVPNQATA
                     PALILVGVMMMASFADIKWLDMEEALPAFFASIFMGLCYSISYGIAAGFIFYTIVKVI
                     KGKYNQVSVALWIVDILFIINFIILATI"
     gene            11891..12700
                     /locus_tag="IXT20_000832"
     CDS             11891..12700
                     /locus_tag="IXT20_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAQ4859299.1"
                     /translation="MNKKLIAIDLDGTTLNQDSMITSKTKETLKKAINAGHHVSIATG
                     RPFRMSHQFYQQLELTTPMVNFNGALVHIPNQHWDGERETLINREIAFEILSQKKQLN
                     LDFIAAENRDTFFIDSFDFFDEKIFASSRPGEKNLLSPKNLTTNPTSLLVRTDKRFAG
                     AVSAELTRQFGSYVDVRTWGGPTAILEIVSKGIQKAKGVQEIANYLSIDQKDVIAFGD
                     EHNDLELLDYAGWGVAMANGTDQLKGIANDVTPLSNQEDGLAVYLEKLLKL"
     gene            complement(12886..15039)
                     /locus_tag="IXT20_000833"
     CDS             complement(12886..15039)
                     /locus_tag="IXT20_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HAQ4859300.1"
                     /translation="MMKDFMKKILNTSWLEKIRKGAQEPKNKTFRRSHVPFRLNFLFF
                     IIFTLFVALIARLGYLQIVNGEEMEARVKSTSTITVQESTPRGMIYDSTGKALVTNKA
                     NQAITYTRGTQVTAKDLLEIATKLNSLIDVPVDENLTDRDKKDFWLADPKHLKEATKR
                     LSAKEKQLGNSEQYSAMVNKVTADEINLNEEQLKIATIFKRMNSAYELNTVYIKNSGV
                     TDQELAVVAERASELPGVSTGTDWTREYNAASSLKSILGSVTTEKQGLPADEAEKYLA
                     KGYSRNDRVGQSYLEKQYEDVLQGTKTQYEVSLDNEGNVSNQKEIFSGEKGSNLMLSM
                     NAEFQSKVEEILKRNYQTLINNGKAQYSPGAYAVAMNPQTGEVLAMTGFSHEQGSKEI
                     TENALGTITSAFDPGSVVKAGTLTAGWASNAISGNQVLIDEPIRLQGASEKSSVFNRS
                     GQVALDAVKASELSSNTYMIKVALKMLGLDYTPGMGLPSLDEEAKAYQQLRDSFKEFG
                     LGTTTGIDLPNESPGISRSVDYMKKFNADNGKEWYAPGNFTDLAFGQFDTYTPIQLAQ
                     YASTVANGGKRVQPHLVKAIYGNDENGNLGEVKKEIGTTVENTVNISAENMAILREGF
                     HQVVHGTDPYTTAKPLASAKMDLSAKTGTAETVAEGHPDIATVNSNIVAYGPTDNPEI
                     AISVVLPNLLDEQDHINLTIAKEIMDAYYDMFMAK"
     gene            complement(15333..16181)
                     /locus_tag="IXT20_000834"
     CDS             complement(15333..16181)
                     /locus_tag="IXT20_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAQ4859301.1"
                     /translation="MSVISRIESMMEDYSSAEKKLANYIIGNIEKVPTMTANELAEAS
                     GLSAPTVVRFSKKIGFQSLTDFKITISTELQTGIDEGFSDIEANESFYSIKNKLGNNA
                     QVAIKETVDILEEETIQKVVECLESAETVFLYGVGASSLVVEDILQKWSRVGKPIIFE
                     KDIHVLLPQLVSNEKKKVLWLVSNSGRSADVVALAELAKSMNIEIIALTQFGNNPLSK
                     IADVLVQTSRPKEITNRSAATNSLLAQFATIDIIFYFYMAKNEKLSEKVKKTREAIQE
                     YFMDSK"
     gene            complement(16405..18345)
                     /gene="thrS"
                     /locus_tag="IXT20_000835"
     CDS             complement(16405..18345)
                     /gene="thrS"
                     /locus_tag="IXT20_000835"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HAQ4859302.1"
                     /translation="MIKITFPDGAVKEFESGTTTLAIAESISKSLAKKALAGKVNGKL
                     VDLTRPIEEDASIEIITPDHEDALGLVRHSAAHLMAQAMRRLYPNIHFGVGPAIDSGF
                     YYDTDNGQNQVTAEDLPAIEAEMMKIVKENLPIERRVLSKQEALEIFASDPYKVELIS
                     ELPEEEVITAYQQGEFIDLCRGPHVPSTGRIQVFKLLSVAGAYWRGNSNNQMMQRVYG
                     TAFFDKKALKEYIRLREEAKERDHRKLGKELDLFMVSPEVGSGLPFWLPKGATIRRTI
                     ERYIVDKEVSLGYQHVYTPIMGDVELYKTSGHWDHYQEDMFPPMDMGDGEMLVLRPMN
                     CPHHMMVYKNTIHSYRELPIRIAELGMMHRYEKSGALSGLQRVREMTLNDGHTFVRPD
                     QIKDEFKRTLELMVAVYADFNITDYRFRLSYRDPNNTDKYFDDDAMWEKAQAMLKAAM
                     DELELDYFEAEGEAAFYGPKLDVQVKTALGTEETLSTIQLDFLLPERFDLTYVGEDGE
                     NTHRPVVIHRGIVSTMERFVAYLTEVYKGAFPTWLAPIQATIIPVSVDAHGDYAYEIK
                     ERLQMKGLRVEVDDRNEKMGYKIRASQTQKIPYQLVVGDKELEDATVNVRRYGSKETA
                     VEDLNIFIDAMEAEVKNYSREN"
     gene            complement(18682..18861)
                     /locus_tag="IXT20_000836"
     CDS             complement(18682..18861)
                     /locus_tag="IXT20_000836"
                     /EC_number="5.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase"
                     /protein_id="HAQ4859303.1"
                     /translation="MPFVHVELVEGRSPEQLENMMKDITEAVHKNTQAPKEHIHVIIN
                     EMKKGTYGVNGEWKK"
     gene            complement(18908..20731)
                     /gene="cadA"
                     /locus_tag="IXT20_000837"
     CDS             complement(18908..20731)
                     /gene="cadA"
                     /locus_tag="IXT20_000837"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HAQ4859304.1"
                     /translation="MTHFKKFLLTLLIGGIALLSEFVFHQPALAFAIVAFTGGILAFL
                     MFLEMIKTLRSGRYGVDVLAITAILATLAVKEYWASLMILIMLTGGDSLEDYANKRAG
                     KELQSLLDNTPRIAHRFKEGKLQDIVIDEINIDDQLLVKPGELVPADGRVTKGESSVD
                     ESSLTGESRPETKKPGDQIMSGSLNGDGSIQMIAEKRAADSQYQMIIKLVEESKEKPA
                     RFVRLADRYAVPFTLIAYLIGGISWWFSKDPLRFAEVLVVASPCPLILAAPIALVAGM
                     SRSSKNGIVVKTGTTIEKLARTKTIAFDKTGTLTKGILEVEAIQPVNGIKKEELLLYA
                     ASAEQESSHILARSLVNNVKNSQLYPVSQLKEVTGQGIQAVVNNHEVKIGRASFVSAE
                     EIETSNTAIYVTIDRSFAGTILFSDQIRPEAAKTIKELRKLDVKNLMMITGDGSAIAQ
                     TIAEKVQLDSVHARSLPQDKLTILESIPKDQRPVAMVGDGVNDAPALAAADIGIAMGA
                     HGSTAASESAYAVILKDDLLRVAKAVVISRDTMRIAKQSVLIGIFICIFLMLIASTGL
                     IPALFGAVLQEVVDTVSILSALRAKKDPAIFPDSVPKTNIV"
     gene            21009..23288
                     /locus_tag="IXT20_000838"
     CDS             21009..23288
                     /locus_tag="IXT20_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAQ4859305.1"
                     /translation="MNEKQLEQTHVDETIELIQIEQNMLDTKQQELTQEMNHSTKDTA
                     NHKIRAGSNEAFYESAVEYRQHEQELLLKYHTLEAQQKRLQTLSVMKDSPYFARIDFK
                     EDRETETLYLGIASLRDLAEETIVIDWRAPIANLYYEGEIGPAFYETETDRIDVDLQL
                     KRQFKIQEGRILSMVDTSEVINDDFLLEILDESSSNHMKNIVSTIQKAQNAIIRDTTS
                     KVMLIEGIAGSGKTSALLQRIAFLLYHNRKWLEAENVLLFSPNHLFSDYISTVLPSLG
                     ESGVPTQTFKNYLVQLLPEFSLLDEQQQESGFLLGEKDPIQVMKSGLTLVDQIDRYIQ
                     GITNYGPLFRDMKINGRTILSKESIRQWYKETNEQLPLHHRLSLLQTKLLKKLGGLQK
                     DETRQQWVKDLAEEQLQELYATDPNLEYTEQKERSLRKKLANQIVRKRFRRIHRGIMN
                     YQFVNITKQYLHFLQTVPLSLLTRHKISETRWQEHLTTVRTHLRQRELRQEDAVLFFL
                     LMRRTYPVTVTHKARYIFIDEMQDFAPSQVALLQALFPKANLTFCGDLNQNVFGNETI
                     VGSLEDLFVDREVTRFQLTTSYRSTKEITDFANHFIVKEHQVETTARKGDLPLLIQGT
                     TDDIKLNWLADTIRTTQGKARYWRTAIIAKTQEECEALYAQLPDELKQQVQLILNESD
                     FMKRSIILLPAFLAKGLEFDRVFLWDVDAEHFSSQQDKLILYTMCTRAMHELTLFTSN
                     NESPLLNNMDISSYEKKVL"
     gene            complement(23381..24028)
                     /locus_tag="IXT20_000839"
     CDS             complement(23381..24028)
                     /locus_tag="IXT20_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4859306.1"
                     /translation="MEAKITLEPFERILSGYRKVEELAVNVTDCSKLAQKYARFGVEG
                     YRLGNYVGTGYLNRYLECMVDRAPMLIYRQNYLIPLLFRRSDSAFRLFEEEYRMEAFF
                     LLLEWSLKHRPEKILIERNEKIDTKKNKVIDSAYLAFRVSEILDCGGYPISNFQSIDQ
                     FIEWNRIYRLIDNGGIGRHSKVFDPEYPENMEELKMIISLVKLKYPETDLDLYIE"
     gene            complement(24207..24926)
                     /locus_tag="IXT20_000840"
     CDS             complement(24207..24926)
                     /locus_tag="IXT20_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAQ4859307.1"
                     /translation="MGRKWANIVAKKTAKDANNSRIYAKFGIEIYAAAKSGDPDPHAN
                     QKLRFVIDRAKTYNVPKHIIDRAIEKAKGAGDETYSELRYEGFGPSGSMVIVDTLTNN
                     VNRTAADVRAAFGKNGGNMGVSGAVSYMFDNTAIFAFAGEDADEILEYLMEKDIDVRD
                     VMEEEGQIIVYGEVADFHSIQEALKEKGITDFSIAEIQMIPQNEVTLSPEDQETFEKM
                     IDALEDLEDVQHVFHNVALED"
     gene            complement(25057..26208)
                     /locus_tag="IXT20_000841"
     CDS             complement(25057..26208)
                     /locus_tag="IXT20_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HAQ4859308.1"
                     /translation="MKSCGIIVEYNPFHNGHRYHVEMARKITGAEVVIAVMSGNFLQR
                     GEPAIIDKWHRANEALQNGVDLVVELPVEWAVQSADYFAKGAVKILQTLDCDSLCFGT
                     EEETSFDYSSFGRFVQENQDRIEEAFQELSDQTYSYPQKMTEVFRSLYPDITLDFSSP
                     NHILGLSYAKENAKYVHPMELHPIRRKSAGYHEKIITDQRIASATAIRHALEEGKRIA
                     GLVPPQTEEDLSTCSLQTWESYWPYLRYRLLSSTLLELTDIYQMTEGIESRMKTAAAT
                     ADSFEDFVSKVKTKRYTWTRIQRLSCYVLLNIKKQEIPVQQNKNYLRLLGFTDAGRRF
                     LKEKKEISIYSRIGKKEAQEAELLVRSDQIYQLGGNIPEQNFGQIPIRV"
     gene            complement(26425..27165)
                     /locus_tag="IXT20_000842"
     CDS             complement(26425..27165)
                     /locus_tag="IXT20_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAQ4859309.1"
                     /translation="MAYETFAFIYDEVMDETLYQQWLAFSKRHLQEDTKNILELACGT
                     GALAVAFAKDGYKVTGLDLSEEMLMIASQRAYEEDASIQFVEGNMLDLSEIGQYQAIT
                     CFSDSLCYMKNRQEVQQVFDEVYQALEENGRFLFDVHSTYQVDEVFPEYSYHYQGDNF
                     AFLWDSYPGSEKHSIEHFLTFFVEEQEGSGRFIREDELHQERTYPLESYLRMLENSGF
                     SKVEAFSDFTDEAASEKSKRWFFVAYKD"
     gene            complement(27169..27492)
                     /gene="rsfS"
                     /locus_tag="IXT20_000843"
     CDS             complement(27169..27492)
                     /gene="rsfS"
                     /locus_tag="IXT20_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HAQ4859310.1"
                     /translation="MEAADSKRAEDIIALDVRNVSLLADYFMICSANSERQINAITEE
                     IIDKEEENKYEVKRIEGKEGGKWVLIDLGDVIVHVFHAPERNFYNLEKLWSDAPLVDL
                     SEWLD"
     gene            complement(27549..28142)
                     /locus_tag="IXT20_000844"
     CDS             complement(27549..28142)
                     /locus_tag="IXT20_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAQ4859311.1"
                     /translation="MNMDYSNEYTAYSRENLMQKIQMRMSEQRFKHVLGVEETAVALA
                     KKYGASPEKASIAALTHDYAKERPDEEFKMVIVRDGFDPELLNYNNAIWHGLVGASFV
                     ERELGITDAEILHAIRVHTTGAAKMSLLDKIIYVADYIEPGRDFPGVQDARAIAWADL
                     DEAVAFETKHTLAHLLAQEQQIYPKTIETYNYWVAKK"
     gene            complement(28126..28773)
                     /locus_tag="IXT20_000845"
     CDS             complement(28126..28773)
                     /locus_tag="IXT20_000845"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HAQ4859312.1"
                     /translation="MNTQAKTFVRSQLFPEEMPQFLEKKKQVGILGGTFNPVHLAHLV
                     MAEQAGRNLGLDRVFLMPSYQPPHVDEKQTIDAKHRLNMLELAVEDNPFLQIETIELA
                     RGGKSYTYDTMKELTQNNPDTDYYFIIGGDMVEYLPKWYKIDELTSMVNFVGIRRPGY
                     TTDTPYPVIWVDVPEIDISSTKIRQKIKEGCSIRYLVPDKVIDYIQNEGLYEYGL"
     gene            complement(28786..29097)
                     /gene="yhbY"
                     /locus_tag="IXT20_000846"
     CDS             complement(28786..29097)
                     /gene="yhbY"
                     /locus_tag="IXT20_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HAQ4859313.1"
                     /translation="MSLRGKQKRFLRSKAHHLQPIFQIGKGGINSAMIVQIEEALEKR
                     ELIKVSLLQNTDEIAEEAAQVLEKEIDCEIVQIIGRVIVLYKPSTKEKYQRLSKEVKA
                     I"
     gene            complement(29113..30222)
                     /gene="yqeH"
                     /locus_tag="IXT20_000847"
     CDS             complement(29113..30222)
                     /gene="yqeH"
                     /locus_tag="IXT20_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YqeH"
                     /protein_id="HAQ4859314.1"
                     /translation="MTEELQCIGCGAVIQTNKPGELGYTPQTALEKGLASGEVYCQRC
                     FRLRHYNETQDVSLTDDDFLRLLNELGQKDALIVNVVDIFDFNGSLIPGLHRFVGDNP
                     VLLVGNKVDILPKSLKKTKLTQWMKERAYEAGIRPVDVLLTSAKKSHEMTELLEKIEE
                     YRDGRDVYIVGVTNVGKSTLINQIIKNTAGVQDVITTSQFPGTTLDKIEIPLDDGHFL
                     IDTPGIIHRHQMAHYLGKKDLRIIAPLKEIKPKVYQLNEGQTLFLGGLARFDFISGNR
                     ASFIAYVSNDVNIHRTKLEKADEFYQKHVGGLLQPPRPDEVEAFPELVRFEFSVKEKT
                     DIVFAGLGWITVTAPVVIAGWAPKGVDVLKRKALI"
     gene            complement(30219..30746)
                     /locus_tag="IXT20_000848"
     CDS             complement(30219..30746)
                     /locus_tag="IXT20_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HAQ4859315.1"
                     /translation="MFSNYKPTWMVEAIYQITPAQLKNLGIKAVLTDLDNTLIAWNNP
                     DGTEELLTWILEMKNAGIPVVVVSNNNSKRVARAIEKFDLLYVARAMKPLARGINIAK
                     KQLDLADDEIVMVGDQIMTDIRGANRAGIRSILVKPIIATDSWKTQFNRFWEKRIMAY
                     LLAKHPDMGWRGGIE"
     gene            complement(30926..31711)
                     /locus_tag="IXT20_000849"
     CDS             complement(30926..31711)
                     /locus_tag="IXT20_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HAQ4859316.1"
                     /translation="MDKTAHDVVQLARAKERWTSLDFFEEIFDSFEECHGDRLFADDE
                     AIVGGIARLDGKPVTVIGIQKGRDLQENIRRNFGSPHPEGYRKALRLMKQAEKFNRPV
                     ITLINTAGAYCGVGAEERGEGEAIARNLLEMSDLKVPIIAIIIGEGGSGGALALGLAD
                     EVWMLEETIYAVLSPEGFASILWKDGSRAAEAAELMKITAPELRELGIVDRVIPETFN
                     GEQLPRYKVAHIMKKALIERLTELVRLDQHKLLEKRYKRFRQY"
     gene            complement(31704..32573)
                     /locus_tag="IXT20_000850"
     CDS             complement(31704..32573)
                     /locus_tag="IXT20_000850"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit beta"
                     /protein_id="HAQ4859317.1"
                     /translation="MALFKKKKYIRINPNRESKPANSPSVPDNMWAKCPNCKHILYTK
                     DIGEEKVCPHCGYAFRIGAWQRLALIIDEKSFEEWDTDLVTKDPLAFPEYTEKIKKMQ
                     DKTGLHEAVLTGKATIQGIPFIIGVMDPNFIMGSMGTIVGEKITRLFERATKESLPVV
                     LFTASGGARMQEGIFSLMQMAKISAAVKRHSNEGLFYLTVLTDPTTGGVTASFAMEGD
                     IILAEPQSLIGFAGRRVIEQTIKQELPEDFQKAEFLLSHGFVDQIVPRMELKQKIHTL
                     LELHTQKGWIDRG"
     gene            complement(32574..33953)
                     /locus_tag="IXT20_000851"
     CDS             complement(32574..33953)
                     /locus_tag="IXT20_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HAQ4859318.1"
                     /translation="MFSKVLIANRGEIAVRIIRACRELGIQTVAIYSEADAEALHTQL
                     ADEAICIGPAKAVDSYLNVQEVLSAAIVTKAEAIHPGFGFLSENSRFASMCEECNITF
                     IGPKSATIDAMGNKINARQLMQEANVPVIPGSTGVLSTVEEALEIADRIGYPVMLKAA
                     AGGGGKGIRKVQSKEELPQHFSSAQQEAAAAFGNDDMYLEKIIYPARHIEVQILGDRF
                     GHVIHLGERDCSLQRNNQKVLEESPSVVISQTKREALGDAAVRAAKAVNYENAGTIEF
                     LMDEEGDFYFMEMNTRIQVEHPVTEMVTGIDLVKKQVEIAAGEPLNVCQEEVVFQGHA
                     IECRINAENPAFHFAPSPGKIQNLLLPAGGMGLRVDSAVYPGYTIPPYYDSMIAKIIV
                     HGNTRFEALMKMQRALSEFITEGIITNAEFQMDLISHPAVIAGDYSTAFLQEEFLPNW
                     TPETEIGGA"
     gene            complement(33961..34380)
                     /gene="fabZ"
                     /locus_tag="IXT20_000852"
     CDS             complement(33961..34380)
                     /gene="fabZ"
                     /locus_tag="IXT20_000852"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HAQ4859319.1"
                     /translation="MNIQEIKEIIPHRYPMLLIDRVEEMVEGERIVAKKNVTINEPFF
                     QGHFPEEPVMPGVLIVEAMAQAGAVALLSLEQFKGKTAYFGGLDKAKFRKKVTPGDTL
                     YLEVEILKVKASAGIGKGIAKVDGKKVAEAELTFMIG"
     gene            complement(34405..34881)
                     /gene="accB"
                     /locus_tag="IXT20_000853"
     CDS             complement(34405..34881)
                     /gene="accB"
                     /locus_tag="IXT20_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HAQ4859320.1"
                     /translation="MDINEIRELVSQFDQSSLTEFDLREGQFELYMNKNKTSRGMNMV
                     QTNETLPVSEPVNVIPEQATTAPEIMKEKESAVFDGIEVVSPIVGIVYLQPAPDKPAF
                     KKVGDQVAKGEVICIIEAMKLMNEITSEADGVITEILVENESVVEYGQPLFRIQQK"
     gene            complement(34884..36119)
                     /gene="fabF"
                     /locus_tag="IXT20_000854"
     CDS             complement(34884..36119)
                     /gene="fabF"
                     /locus_tag="IXT20_000854"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HAQ4859321.1"
                     /translation="MNRVVITGYGVTSPIGNDADSFLNSLKEGTNGIAPITKFDASET
                     GITLAGEVKDFPYDKYFIKKDSKRMDMFSIYGIYAALEAMEMSGLDKEKMNQDRFGVI
                     IGSGIGGLPTIENQVIRLHEKGAKRVSPMFVPMAIANMAAGNIALRVGAKGICTAIVT
                     ACASGTNSIGEAFRNIKHGYSDVILAGGTEATICEMGIAGFAALTALSESTDPNRGSI
                     PFDENRNGFVMGEGAGVLVLESLEHAQARGAKIYGEIVGYGANCDAYHMTAPTPDGSG
                     ASKAMKLAMEEAGVQPEQVGYINAHGTSTPANDQAEAKAIQIALGENFRDTYVSSTKS
                     MTGHLLGAAGGIEALATLLALEHQFIPPTINVEKQDPEIDLTVVKNESKPASFTYAMS
                     NSLGFGGHNAVLCMKRWEE"
     gene            complement(36138..36875)
                     /gene="fabG"
                     /locus_tag="IXT20_000855"
     CDS             complement(36138..36875)
                     /gene="fabG"
                     /locus_tag="IXT20_000855"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HAQ4859322.1"
                     /translation="MDVKGKNVFITGSTRGIGKAMALAFAKAGANIILNGRGEIPKEK
                     IEEIEAFGVKCVGVSGDISDYEKAGQMIKEAEEKLGSIHVLVNNAGITNDKLVMRMDA
                     EDFKKCLDINLIGTFNMTQHVLKKMMKQREGAIINLSSVSGLIGNIGQANYAASKAGV
                     VGLTKSVAREAATRGITCNAIAPGFITTDMTEVLADKVKEQAEKQIPMQRFGQVEDIA
                     QTAVFLAQNPYITGQVINVDGGLVMHG"
     gene            complement(36887..37804)
                     /gene="fabD"
                     /locus_tag="IXT20_000856"
     CDS             complement(36887..37804)
                     /gene="fabD"
                     /locus_tag="IXT20_000856"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HAQ4859323.1"
                     /translation="MKTAFLFSGQGAQYQGMGKDLYEEAVVKQTFDEASEILGYDMAE
                     LCFTENERLDQTQYTQPAILTVSIAYYRLLKEHGIIPDAALGLSLGEYSALVASNALS
                     FTEAVALVAKRGAYMTEAAPAGSGKMVAVMNAPIETIEESCHEASKYGIVSPANYNTP
                     QQIVIGGEEKAVDEAVLLLKEKGFKRMIPLNVSGPFHTAILEPAAKKLAKDLSQIQFS
                     EPSFPIISNTTTEIMKKETIAGLLEQQVMQPVRFYESIHKLKTIGIEQVIEVGPGKVL
                     SGFMKKIDKTIPVLRVENKQTFDETIAIL"
     gene            complement(37911..38135)
                     /locus_tag="IXT20_000857"
     CDS             complement(37911..38135)
                     /locus_tag="IXT20_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HAQ4859324.1"
                     /translation="MVFEKIQEIIAEELGKETEEIKLTTDIQEDLEADSLDLFQIINE
                     IEDEFDVKIETEDGIKTVQDLVTYVEKQTA"
     gene            complement(38188..39150)
                     /locus_tag="IXT20_000858"
     CDS             complement(38188..39150)
                     /locus_tag="IXT20_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HAQ4859325.1"
                     /translation="MEQFGMITATASVLPEKIITNNDLSKMMDTSDEWISSRTGIRER
                     RCVTNQETSDLCILVAEKLIKQKNLNPKDLDFILVATMSPDYTTPSVAAQVQGKLGAT
                     HAIAFDISAACSGFVYALSMAEKMIRCGSTKGLVIGGETLSKLIDWSDRTTAVLFGDG
                     AGGILLEANPQKKLLKEKLAADGTRSSSLTAGYQQNKNPFYSEDSEGSYYLQMTGRDI
                     FDFAVRDVANNIQEVMKDKPVDYLLLHQANLRIIEKIARKVKMPQEKFLTNMDKYGNT
                     SAASIPILLDEAVSSGKITLGKQQKVIFTGYGGGLTWGSTLMEL"
     gene            complement(39160..39600)
                     /locus_tag="IXT20_000859"
     CDS             complement(39160..39600)
                     /locus_tag="IXT20_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAQ4859326.1"
                     /translation="MEPDLKTVNDYLVSVFNDILTIEESELKKSQFKDLSITEMHTIE
                     AIGMYKKKTTSEVAKELSITVGTLTTAINKLVKKGYVERIRSEDDRRVVKLGLTKKGK
                     LLFRVHQHFHREMVKNILDGMATEEQHALLDALKNLHDFLQDYK"
     gene            39906..40871
                     /locus_tag="IXT20_000860"
     CDS             39906..40871
                     /locus_tag="IXT20_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HAQ4859327.1"
                     /translation="MLKEKMKTLPMLPGVYLMKDRDGKVIYVGKAKRLKNRVSSYFHQ
                     NKQHSKKVLRMIHHITDFDFVVVDTELDALLLECQLIQHYRPFYNRQMNYFSNYNYVH
                     ITNKGFVLTDTPTARTYGPFRLYKKMPSILRIMEETYQMPWLSEISLLALRVQLPDLQ
                     EMTFEQKKKELQGLFQGRNKKLLTYLKKRQQHFIYQLNFEKAGMLQKDIELVTYFIRR
                     IQEQKQFLRTPSLTFSMPLAADESQKKHYLVCYGQLAETMITSGDVSPDFYYEKKEAH
                     LSLKRQLSKEEIDPVQILISYRKKLEKEQFEIELLNKKEAEKQLN"
     gene            complement(40928..41158)
                     /locus_tag="IXT20_000861"
     CDS             complement(40928..41158)
                     /locus_tag="IXT20_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkuJ family protein"
                     /protein_id="HAQ4859328.1"
                     /translation="MTHSQLVAIIKRLEAMVESADNELQVRRFEKEGVEKCTVTYDKA
                     TDTFELTETDTHQQYEFDNIDIVAMEIYDLIQ"
     gene            complement(41244..42131)
                     /locus_tag="IXT20_000862"
     CDS             complement(41244..42131)
                     /locus_tag="IXT20_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HAQ4859329.1"
                     /translation="MKLGFIGTGVMGSAVARHLLEAGHEVAVYNRTKAKADPLVTEGA
                     IWADTPKAVAEQSNILFTMVGYPKDVEEIYYGQSGIFSADISGHILVDLTTSTPSLAE
                     KIAKTAKEKGADALDAPVSGGDLGAKNGTLTIMVGGEEAVYDQVLPLFKEFGTTFTLH
                     GSAGKGQHTKMANQIMIAGTMTGMTEMLVYAQKNGLDLKKVIETLSGGSAANWSLSNY
                     SPRILKEDYTPGFFVKHFIKDLKIALEEAEKMDLVLPATTQALKLYEELADKGFENDG
                     TQALIKLWWPEGKIPEKKS"
     gene            complement(42220..43443)
                     /locus_tag="IXT20_000863"
     CDS             complement(42220..43443)
                     /locus_tag="IXT20_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HAQ4859330.1"
                     /translation="MKIGFFTDTYFPQVSGVATSIKTLKQELERHGHEVYIFTTTDPN
                     ADKLEKDVIRMPSVPFISFKDRRVVVRGMWYAYLVAKELELDLIHTHTEFGAGILGKM
                     VAKKLKIPLIHTYHTMYEDYLHYIGKGKVIRQSHVKYLSRLFANHTTGVVCPSERVID
                     TLRSYGVIAPLRVIPTGIDIEKFKRSDITQQDISKLRESLGLQENHLMILSLSRISYE
                     KNIQALINGFPQVISAYPNARLVIVGKGPYVEELEELISELQLEEFVQFTGEVDNNDV
                     ALYYKAADYFVSASTSETQGLTYTEAMAAGTPCVVEGNAYLNRLFDHPSLGKTFETDE
                     DFAPALIDYISSEVPKDPKVLEAKLYDISSTHFGEAMIEFYQDMVVYHEEEMRRKEEE
                     DSIERIKIKLNSFKR"
     gene            complement(43460..44539)
                     /locus_tag="IXT20_000864"
     CDS             complement(43460..44539)
                     /locus_tag="IXT20_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HAQ4859331.1"
                     /translation="MLYYISEGGRKVKVLLYFESEKMLAKSGIGRALDHQKRALTEVG
                     ISYTLDEKEDYDILHINTYGINSHNMVNKARREGKKVVYHAHSTEEDFRNSFIGSNQL
                     SPIVKKYLVGLYQKADYLITPTPYSKQLLESYSIRVPIQAISNGIDLEKYRPDPMKEQ
                     KFREYFKLSPDQKVIICVGLFFERKGIIDFVEIAKKMPEYTFIWFGHVPMYSIPRNIR
                     KIVKEDHPENVLFPGYIRGEIIEGAYSGADLFFFPSYEETEGIVVLEALASKQNVLVR
                     DIPVYNGWLEDSKNCYMGKTNEEFIRLIQQITNQELPSTTQAGYQTAKERSINKIGEE
                     LKNVYESVLNEKVSSKLKKVNQVKD"
     gene            complement(44670..46397)
                     /gene="ptsP"
                     /locus_tag="IXT20_000865"
     CDS             complement(44670..46397)
                     /gene="ptsP"
                     /locus_tag="IXT20_000865"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HAQ4859332.1"
                     /translation="MVEMLKGIAASDGVAVAKAYLLVQPDLTFSKATVEDTAAEEARL
                     DAALAKSTEELQQIREKAAQSLGEAEAQVFDAHLMVLSDPEMVGQIKQNIKDNSVNAE
                     SALKEVTDMYIGMFEAMEDNAYMQERAADIRDVAKRILAHLLGVTLPNPSMINEEVVV
                     VAHDLTPSDTAQLDRNFVKAFVTDIGGRTSHSAIMARSLEIPAIVGTKEITAKVKEGD
                     ILAVNGIEGDVIIDPTDEQKAEFEKAGADYAAQKAEWEKLKNAETVTADGKHFELAAN
                     IGTPKDLVGVHNNGGEAVGLYRTEFLYMDSPDFPTEDDQYEAYKAVLEGMEGKPVVVR
                     TMDIGGDKELPYLQLPHEMNPFLGYRALRISLSEQGDEMFRTQMRALLRASVHGNLRI
                     MFPMVATLKEFRAAKAIFEEEKQKLISEGKEVSDTIQVGIMIEIPAAAVLADKFAKEV
                     DFFSVGTNDLIQYTMAADRMNERVSYLYQPYNPSILRLIKNVIDAAHAEGKWAGMCGE
                     MAGDQTAVPLLVGMGLDEFSMSATSILKTRSLMKRLDTAKMAELADRALKECDTMEEV
                     VELVHEYVK"
     gene            complement(46397..46663)
                     /locus_tag="IXT20_000866"
     CDS             complement(46397..46663)
                     /locus_tag="IXT20_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010773230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr"
                     /protein_id="HAQ4859333.1"
                     /translation="MEKKEFHVVAETGIHARPATLLVQTASKFNSDVNLEYKGKSVNL
                     KSIMGVMSLGVGQGSDVTITAEGADEADAMAAIVETMKKEGLSE"
     gene            47142..49376
                     /locus_tag="IXT20_000867"
     CDS             47142..49376
                     /locus_tag="IXT20_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HAQ4859334.1"
                     /translation="MLCQNCGKNEATIHLYATVNGQRTQLDYCQSCYQKIKNQQNGGL
                     TNMAQNDPFGFGSLDDLFRSMSRQMQQQGNYEQTPPTQFGGNNNFNGGQPPQGNTEGL
                     LGEYGINITEQARQGDIDPVIGRDDEIKRVIEILNRRTKNNPVLIGEPGVGKTAVVEG
                     LAQKIVDGDVPQKLMDKEVIRLDVVSLVQGTGIRGQFEERMQKLIEEIRQAENVILFI
                     DEVHEIVGAGSAGDGNMDAGNILKPALARGELQMVGATTLNEYRIIEKDAALERRMQP
                     VRVDEPTVEETISILKGLQKRYEDYHHVKYTDEAIEAAATLSNRYVQDRFLPDKAIDL
                     LDETGSKKNLTIQIVDPKTIEKKLQEAEEQKVLASREEDFEKAAYYRDQINKLQKMKE
                     RQLTEEETPVITEKDMEKIVEQRTGISVGELKEKEQTQLKNLADDLKAHVIGQDNAVD
                     RVAKAIRRNRVGLNKQNRPIGSFLFVGPTGVGKTELAKQLAYELFGSQDSMIRFDMSE
                     YMEKHSVSKLIGSPPGYVGYEEAGQLTEKVRRNPYSLVLLDEVEKAHPDVLHMFLQIL
                     DDGRLTDAQGRTVSFKDTIIIMTSNAGTGKVEANVGFGAAREGVTRSVLNQLNNYFTP
                     EFLNRFDGIIEFSALSKENLMTIVTLMLDDVNQMLAAQQLHIEVPTNVKEKLVDLGYD
                     PSMGARPLRRTIQEQIEDGIAEFYLDHPSIHELKAKLDKDGKIIVTSKPERLAKESAE
                     ETAE"
     gene            49524..49844
                     /locus_tag="IXT20_000868"
     CDS             49524..49844
                     /locus_tag="IXT20_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HAQ4859335.1"
                     /translation="MKLVNVTNSHSRLVLNQLENTDAHLVKVYTAGNTTIVYTEAPEH
                     NEILLINDKRKIQPKEIEDAKNYFLDKIKDAVYHEDEIKVIELAGLVEISIPKHELGA
                     TLAV"
BASE COUNT        14244 a        10705 c         8723 g        16193 t
ORIGIN      
        1 atatttttta tttaccgact acaaaaaaat aaacctcaag gctccatggt tagccttgag
       61 gttttacatt ttctaattaa aatgtattaa gcttcttttg acattacgtc cttaccatca
      121 tagtaaccac aatttgcaca tacgtgatgg ctttttttca tttcaccaca gtttggacat
      181 tcgtttaacc ctttgattgt caatttgtag tgtgtacgac gttttgcttt tttagctttt
      241 gatgttcttc tagctggtac tgccattttg ctacacctcc ttgtaaagtc ttgtatctaa
      301 attagacaca atatattcca atcttacttg ttatcgtcgt cttttgattt agaatcaaaa
      361 agttcagata gttttgcaag acgaggatca attttattct cggcttcttc catttttctg
      421 cgctggtatt cttcttcgga aacaacttcc caatcattgc cttttggaag ttctgttgct
      481 gcttcctctt cttttgtcaa tacccgcatt ggaatcgcca ataagatgtt gtcttcaatc
      541 gactctgcta aattcaataa tggaccttca attagcaaaa tctcatctgc agacatttgt
      601 tcatctctac tttggaattg ttcttctgtc atgaatactt cgtctacaga aaaatccatg
      661 ggcagttcaa caggttctag tgaacgtgtt gaaggaactg tcaaagtcgt ctgcacagtg
      721 taatgaacga tatagccttt cttatcaact gagaccaaac cttcgacttc cactggcgca
      781 acatcaagta ttaaattgtc tcgcttcatg agagcggctt tcaagtcgag cgtctcatga
      841 aaagttaacg gtgtctcctg gtattttctc aattccaata aagaccattt catttttcat
      901 cactcctaag caacaaaagc aattatacag ggcaaaaagc atgttgtcaa tgaaattttc
      961 ttgacacctt gcagttcata aaaaaacaga aaaatcttta ttagaaagcc tctgtttgct
     1021 atcatcctca gaatcattta caataatcat tattccatta aaggagcgga tgatcttcat
     1081 gaacacgaaa gatatcgaat actatgtcca aatcgtcaaa caaaaaaatt ttacgaaagt
     1141 ggctaattat ttccgtgtga gccaacctac tattacatat gcattacaac gtcttgaaaa
     1201 agagtttgac accgtactta ttacaagaga tcgatcgcat cacgaattaa tcgtcacgcc
     1261 ttctggaaag cagctgtttc tccatgcaca aaccattctc caagagattt ctactgcaag
     1321 agctgaaatc actcagttgc aaaagaaaaa gcttcgtttt ggcatgcctc ctattatcgg
     1381 caatgaatac tttcctaaac tgtcaagtta ctttatcaag catgaattga tgaatcaaat
     1441 tgagattacc gatggaggct ctcgggactt atacgggatg atccgacaag ggaggatcga
     1501 tttagctgta ttaggctcaa cacagccaat cgtggatcaa tatttagaaa cagagttact
     1561 tttggacaaa cgtttcatga tcgtcgtgcc acctacacat cctctttcca agcaggatac
     1621 tgtctctttc aaagatatag cagatgaaca gtttgtacta ttgaacgaac attatgtcca
     1681 ccccgcagca ttcactaagc tttctaaaca aacacatact catccaaaag ttgtttatca
     1741 aagcaatgac ttgacgattt taaagagtat ggtccgagaa aatgtgggta tcggtttttt
     1801 ggcagagatt gctgtccatc ctgaagatca tttagtttgc ctccctcttt cagatgaagt
     1861 tcagcctcgc ttcttgattt cattggcaaa acgaacacag cagatcgtca cgcctcttca
     1921 agaacaagta gtccaagtta tccatgaatt cgttacagca caaaaaaaca gctcttcgta
     1981 aaagaagagc tgtttttatt ttttattaag ctaaattaga tagctgttgt ttcgccacgg
     2041 taaacgatgc cgcgacgtga atcaacggta atcaattcac cttctgtaat tgttgttgtt
     2101 gcgcctttag caccaacgat tacaggaata tcttgtgcaa ttcctacaac tgcagcgtga
     2161 gaagtcaatc cgccttcttc aacgataaca gcagctgctt tttcgattgc aggcatatat
     2221 tctttgtcag ttgatggtac aactagaacc attccgtctt ttgctttttt tacagcttct
     2281 tcagcagatg aagcaataac tgcatttgca acgacagatt tttgtccaac gccttgagct
     2341 tctaataatt tagaaccaat catttgaatt ttcatcacgt tagttgttcc gcgttcacca
     2401 actggtacac cagcagtgat caagattaaa tcgccttctg atgcgaaacc taattcaaca
     2461 gcttttttag ctgctagttc aaacatttca tcagttgttg atggtttttc agcaactgtt
     2521 gggtaaacgc cccagtttaa catcaaacca cgttttgtgc gttcgtcaaa agtaacagcc
     2581 aagatatctg catctggacg atattttgaa atcatttttg cagtgtatcc tgattcagtt
     2641 gcagcaacga ttgttttcac gcctagattt tttgcagcac gagctacaga taaaccgatt
     2701 gtttcagtta catctgtttt atcgaattcg ttcaattgga atgtacctaa ttctgataat
     2761 gcattttcag cttcttggtc gatacgtgcc attgttgaaa cagcttcaac tgggtagtcc
     2821 ccgtttgcag attcaccaga tagcattgtt gcatctgtgc catcaaatac agcgttagca
     2881 acgtcagaag cttccgcacg tgttggacgt gggttttctt gcattgattc taacatttgt
     2941 gtagcagtaa taactgggat accagcagca ttacattttt tgataatgcg tttttgaacc
     3001 attggtacta gttcagctgg gatttcaaca cccatatctc cgcgggcaat cataacaccg
     3061 tcagaaactt tgatgatttc gtcaatgttg tcgatacctt cttgagattc gattttaggg
     3121 aagatttgaa catgagtcat gtctttttct tctaagattt cgcggatatc tagaacgtct
     3181 tgtgctttac gtacaaaact tgcagcgata aagtcgatat cgttgtctaa accaaaacga
     3241 atatcgtcag catcttttgg agtaatacct ggcaaactaa tagaaacgcc tggtgcattt
     3301 acccctttac gtgatcctag catacctgca tttttaactt ctacgactaa ttcacggttt
     3361 gcttcgtctt tttcaatgat ttgcatatcg atcaaaccat catcaaataa aatgtgtccg
     3421 ccaacgtgaa catcgtcaaa taatcctggg taagtaaccg cgattttttc ttttgaacct
     3481 acatgttcag gatccataga gatgcgtgtt tgatctccta cttcaaattt gatgtatcct
     3541 gcacgaccaa aatcagcttc tgttgtgcct tgaacagttg tacggatttc agctcctttt
     3601 gtatctaaaa gaataccgac atcttttcct gtctttttaa ctgcttcacg gaccatagtc
     3661 atacgtgata attgttcttc atggtcacca tgagaaaaat taaagcggaa gacatttgcc
     3721 ccagattcaa tcaattgaga aataatttct actgagttac ttgctggtcc taatgtacta
     3781 acgattttgg tttttttcat ttaaaaaact ctcccattca attttgttta tttaaagctg
     3841 tacacagctt aaatagcttg gtttctttca ctcttaaaat gaaagtgaat tatttagatc
     3901 atatagtgat aagtctggtt tatgtttgtt gttttcaagt gtatcaatga tgtcagacgc
     3961 aacgatttcg ttgtcatgga taccgacaca taatccacct tttccttctt gaagcaattc
     4021 gacagcgtaa gcaccgaatt tacttgccaa cacgcggtcg cgtgcgcttg gtgctccacc
     4081 acggacaacg tgtcccaaga tagatacacg tgtatgatag tcaccaaatt ctgaaagttt
     4141 ctcagcgaat tcatttccgc ccattacacc ttcagccaag atgatcaagc aatgtttctt
     4201 cccgcgatcg cggccttctt ggattttttt agcaactgaa gccatatcaa aatcatgttc
     4261 tgggatgatg atttcatccg ctccgccagc cacacctgac cataaagcga tgtctcctgc
     4321 attgcgtccc attacttcga taacgaatgt acgaacatga gaagtcgctg tgtcacgaat
     4381 acggtcaatt gattctagta ctgtattgat tgctgtatca aaaccgattg tgaaatcagt
     4441 cccaggaata tcattatcga ttgttccagg aatacctact gctgggaaac cgcgttttgt
     4501 caaagccatt gcgccatgat aagagccgtc tcctccgata accactagac cttcgattcc
     4561 gaattttttc aattgttcga ttccttttaa ttgaccttct tcagttgcaa attctggata
     4621 acgtgcagaa tacaagaatg taccaccacg ttggatcttg tcacctacat ctgccacatc
     4681 aagacgacga atatctccag caactaaacc ggcaaaacca taattgatcc cgtatacttc
     4741 cagtccttcg taaatacctt tacgaacaac ggcacgaaca gctgcattca ttccaggtgc
     4801 gtctccgcca ctggtcaaaa ttccgatgcg tttcataata aacattcacc tcattaaaat
     4861 actttatcct aagtatgact taggtttaca accatttttg cgttaaccac gactaacatt
     4921 ctaacattat ttttctcact tgtcttgatt tacggataaa aaaatgaaaa aaaatgaaag
     4981 caagtagtat tttttaccgt aagttatttg aaaaccacat tatcttcgcc aagcagataa
     5041 ataagttgcc cctttatact cttgctgtca tccacccaat aagcatctga cagaactact
     5101 tttcgttgcg ttttttcgtg atacataact actggtatat atcctgggtg tttctgaaaa
     5161 atcacttgta attgtttcat tatctcggga tcttcccgtt cttctgtaat acgcaaatag
     5221 caagtttgtg aaggatactg ggcttctaac tggttaggat cagcaaagct ttctccaatt
     5281 agttgcagtt cctgattata tttgctgatt tcgctccgcc cttgaacaaa gatcactcga
     5341 tcaacggcaa acaaattacg atatttacga taaagagttg gaaaaactgt gacagaaatt
     5401 tctccactac tgtcggttcc ttcaacaaag gccatcagtt ctcctttttt tgtccgaatt
     5461 tctctaactt ccttgatcat cactaataaa ttgactggtt gattagggac gatatctgta
     5521 atcagctgaa cttgtttagc taaacgaaga cgatcaaacc cttctgcagg atgacctgac
     5581 aaataaatac ctaagtactc ctcttccagc ttcaagcgtt cttctagtgt atagtcagcg
     5641 acttcttctt cttttaatgc catgattccc agcaagtcaa gacttccacc actgtaaacg
     5701 atattttgga tttttccttc gagatcaagc atcaattgtt tcctgttagg agataattca
     5761 tcaaaaactc ctactgctgt cagtgattgc agtaactcta atttcagcca acgcttatcg
     5821 atccgtatga ggaattgatc cactgactgg aacgggccat tttgttttct ttcgtttaat
     5881 atatcttcta caaaatcacg ccgaatacct tttatcgctg tcagtccgaa tctgatttct
     5941 tccttattga ccaactcaaa actggaatta ctttgattaa tggacggcga ggaaagtttg
     6001 agtttatttt tacgtgcttc tgcaatatat tctttcaatt ttgatgtgtt atgccgaaca
     6061 gaattcatca ggctggtaaa gaaagcacct ggatgatggg cttttagata cgccatttgg
     6121 aatcccacga atgaataagc aaatgcatgt gaccgattga atccatagtt tgcaaatttt
     6181 tcgatatatt catatacggt cgctgctgtt ttttccgaat atccctgttg taatgctcct
     6241 gatacaaaat gcgacttttc ttcatctaat acgtcttttt tctttttact cactgcccgt
     6301 cgcagaatat ctgactgccc taatgtaaat ccagccatag tcgaggcgat ctgcatgatt
     6361 tgttcctgat agacgatcac tccatatgtg tttttcaaaa tcgggatcag tgaatcatct
     6421 gggtagcgaa tggcttcttt tcctttcttc ctggcaatga aggtatctat attttgcata
     6481 gggcctggtc gatacaatgc attgactgcg gcaatatctt cgatgttttc tggatctaat
     6541 cttcgtaaaa cgttccggat accagcagat tcaaattgaa atactcctga ggtttcgcct
     6601 cgttgaaaca agagcagcgt ttttgcatca tttagcggga tctcttttaa tcggatctca
     6661 tacccttcta ctcgcttaac agctttcaaa gtaaaatcaa taatcgataa gttgcgtagt
     6721 cctaaaaagt ccattttcaa aagaccgatt gcttctacct cattcatcgt atactgggtc
     6781 agaaaagctt catcagaacc gcgttggagc ggaactagtt caagtagatt cttatcgctg
     6841 ataactacac ctgctgcgtg agtcgaaaca tgtcttggca atccttctaa aatcactgca
     6901 gttttgaata aaagttggtt tttttcgctt gcctgaacta attctactaa ttgatgcgaa
     6961 gttttataag cttgggtcaa tgtgattttc aattgattgg gtattgcttt tgaccagcgg
     7021 tttgcttcac tttgcgataa accaaacact ctagctgcat ctctaagaac catttttgct
     7081 gccattgtgc caaatgttgc gatttgtgcc atatgatatt gtccatactt ttctcgaaca
     7141 taattcaata cttcttcacg tcgattgtca ggaatatcca aatcgatatc tggcatcgta
     7201 aagcgttcgg ggtttaaaaa gcgttcgaac agcaaatcat attctaatgg atccacatct
     7261 gtgatttcta acacataggc tactaatgaa cccgcagcag aaccacgacc tgcaccggta
     7321 acgattttgt ttctatgagc aaaagccatt acatcccata cgatcaagaa ataatcatcg
     7381 aatcccattt gatggatgac agataactcg tagtccaaac gttcttggta ttcctttgta
     7441 tagtcagtga ttcggcgttc cacttgcgaa tagcataact ctcttaaata ttctccagct
     7501 tgttttccat taggaactgg ataatgcgga agtaatttct gatggaactg gaattcaaat
     7561 gaaatcgatt cagctaaacg ctgggcgcct attacagctt gggaaagacc cgctttttcg
     7621 aatcgtttga tcatttcttg ttctgaaaga agatagacat ttgctgttag tggtaaagat
     7681 gcagataaat tttctaaagg tatttggctt ccctctttga tatgtttcat cacttcaagt
     7741 gcaaaaagtt cttcttgttg taaactagcg atgggatgta atgcacaaag cgaataattt
     7801 tcttttatca catactgata ccattcaggc aattgatcag gcgtgttttc tgtcaagccg
     7861 atataaaaat ttctagtaaa aacattttca agtaaaaact gccaatgacc gaaaaagtta
     7921 tcctctttat tgaaagcctc agaaacagaa tgatttgccg gaacaacaaa cgctagatgg
     7981 tcggtttctg tcatttctgc aagttccaat ttttcttgga tcatttttcg gctggaaagt
     8041 ttcatcagct gctgataacc tatttcatct tttgcgtacg caagcaagcc ataatttttt
     8101 tcttcataag tataactaag atgtaatcca atgattggct gtactccttc ttctacacaa
     8161 atacgataga attcaggtgc accgtataag acatctttat ccgttatggc taatgaagtg
     8221 tagccttgtt tttttgcttc ttgtacatat tctcgaatac gtatcgtact ttgtaaaaga
     8281 gaataagaag taattgtata aagttgtggg aaaaacatct tgcattccgc cttttttttc
     8341 gttaaaattc actagcgtta aaaggtttga tccttttact ttcattaaga tagcaggcgc
     8401 tgaagttgat tattccactt catttgcttg cttgtaatct gtaggaatga gttctttcgc
     8461 aagaactcaa attttattga gatttggtct cgtttcttta cattcaaaga aaatctggta
     8521 aaattaagat agaaaagagg tgttctttat gcgatatatc gttacattat tctgggctgt
     8581 cgttctaggt caagtagtcg gttatattgg tgctgcatta acaagcggaa catatgattt
     8641 tactttgaca acaatcattt cattcattgc gggtgtcatt atccttttga ttggtgcagt
     8701 tgcgccaaga aaagaaacct ctgcccattc gtaaaaaaac agccaagagc ttgagatttc
     8761 tcaagctctt ttttattttc tactcctcac caccattata atctgctgcg aagaacttta
     8821 cgaaagttga ataaagaaag aagaatattt gacagaacaa acaaacttgt cacaataaaa
     8881 acagcactgt aattataagc atgcgcgact gtcgatccta gcatgggacc taaaacttgt
     8941 ccaaagttgc taaacatctg attgtagcta tagattcggc tgactccttc ttgaggcgta
     9001 atcttactga tcagtgtatt gacagaaggc atcaaagcgc cagttgaaaa gcctaaaata
     9061 aagcgaagta tacctaattg gagcggtgta gtgacgaatg ccataggtaa ataacagcaa
     9121 aatgataaaa tgagtcctcc aattagaact ttttgattcc caatcttatc ccctagtttt
     9181 cctagtttcg gtgaagaaaa gacagcggaa acaccagcta tcgaaacaat caagccgctg
     9241 acaaaaagga tattttctgt acttccgctc aattcacgaa tatataaggt caaaataggg
     9301 ctgatcgtag tgattcctat ctgtaaaatc aaggtcgtaa tgaagagacc gatcaaaata
     9361 gagagatgat ccatttttga aaaaatttct ttggtgctta tcagatcttt tttctcgatt
     9421 ggatggaaat cttctttgac taagaaaatg gtcaataccg ttgtgatcat caacaaagcc
     9481 cctgtgatca agaatacatt ttccatacca aaccactgag ccagtgcacc gcccattgaa
     9541 ggtccgatca gatttccagc aatcgctccg gtagacaatg ttcctaacgc ccatccgctc
     9601 ttttctctag gtgcctgtga agcaatcatc gctgtggcat ttggaatata tccagacaaa
     9661 atcccattcc aaaaccgcat gatcaaaagc caatacgcgt ttggtacaaa agccaaagat
     9721 cccattgtta atgtcatccc tgctgctgca cggatcatca tgagttttcg gccttttcga
     9781 tcagctagat ttccccagat tggtgcaacc acggcagatg caaaagcagt cacagagatt
     9841 gctagtcccg aaaacaattc gatttgatct tttggtgttc ctagttgctc aacataaact
     9901 ggtatgaatg gcataactaa actaatactt gcaccagtga aaaaacagcc aatccacgca
     9961 atcactaaat tttttctcca attaatctcc atctatcatc cctctttcgt ctcttttatc
    10021 atacctattt tcgaataaac aagcaacgat ctcgccagta aaaagagaaa aatcccgcaa
    10081 aggtcatttt atatgtaacg ttctttttat gtaaaacaaa aagagatcag aacaaatact
    10141 gttccaatcc cttctcttta gttaaattgt tgctagaatg atgaagttga taatgaataa
    10201 aatatcaacg atccacaaag caacagaaac ttgattgtat tttcctttga ttactttcac
    10261 aattgtatag aaaataaatc ctgctgcaat cccataggaa atgctatagc acaatcccat
    10321 aaagatcgat gcaaagaagg ccggtaaagc ttcttccata tctagccatt tgatgtctgc
    10381 aaaagaagcc atcatcatta ctccaactaa aatcaatgct ggcgcagttg cttgatttgg
    10441 tacaatggcg atcagtggag agaataaact acttaatgcg aatagcactg ccactactac
    10501 tgaagtcaaa ccagtacgtc caccggcccc gatcccagct gcagattcga cataagttgt
    10561 tgtattcgat gtaccaaaga tagcccccac agaagtagcg atcgcatcgg caaatagtgc
    10621 tttatccatt tttgttttga acccacgacc atcttccaac gcgatctcgt cttcttttga
    10681 gaaaatacct gtgcggcgtc ctgtaccgat aaatgttccg atcgtatcga aagtatcaga
    10741 taaactgaat gcgatgatcg tcatcaatac ttgaggaatc ttggaagaat cactgaataa
    10801 agattgcatg ccttcagaac caaatgctgc accgaaagtt gttcctaatt ctttaaatga
    10861 attacctaac gaattcgttt gccaatcaat ggaacttaaa tcaacaacac ccataaaaat
    10921 accaaggact gtggtcgcta cgataccgat aagaatagcc ccacggacat tttttacaac
    10981 taaaatggct gtcagtacca aaccaatcac agccaataag ataggagcgt tgtcaaagtt
    11041 ggctaacgca gggacgattc caccatttat cgtaacattt gtcgcttttc cgccttcaac
    11101 gacagagctc gaaatagctg actggtctgc tgaaaaagat aagaatcctg catttttgat
    11161 tccgacatac gcaacgaaaa taccgatccc gccaccaatc gcatgttgaa gactttcggg
    11221 aatcgcccga ataatcattt ttcgaatatt tgtgacagta ataaaaatat tgattaatcc
    11281 gcaaataaac accattgcca aggcttgttg ccaagaataa ccaagcccaa aaacgaccgt
    11341 aaaagtgaaa aatgcgttca atcccatacc aggtgcctga gcatatggta catttgcgaa
    11401 caatcccatt accagcgtac caataatcga tgcaatgatt gtcgctagaa atactgcttg
    11461 gaagggcata cctgatgctg acaaaatcgt cggatttacg aataagatat aactcatggc
    11521 aaagaatgtc gttactcccg ccattatttc agttgataca tttgtattgt ttttctttaa
    11581 accgaaaaac ttttccataa aaagttccac tccctgaatt tttttctgat ggaaaactat
    11641 aataataaaa aataagtaaa atagggcaaa tccatcgaac aatttcaaat tatatagacg
    11701 tttcattcgc ttttcaacgt ttttttaaca aaaaaagcga aaataatact taacgttcgt
    11761 gtttagctta tgtttagcta tttatatagc cataactgta gtttttgtta ggaatagctg
    11821 attgctcttg ttaattacct aaaatatgct agactaaaac aaatgctagt tgaggaagga
    11881 aggaccctta ttgaataaaa aattaattgc catcgatctg gacggaacta ccctcaatca
    11941 agattcgatg attacatcta aaacaaaaga gactctaaaa aaagcaatca atgccggtca
    12001 tcatgtcagt attgctacag gacgcccatt ccgaatgagc catcagtttt accaacaatt
    12061 agaattaaca acaccgatgg ttaattttaa cggcgcatta gtacacatcc ctaatcagca
    12121 ctgggatggt gaaagagaaa cgttgatcaa tcgtgaaatc gcttttgaaa tcttatcgca
    12181 aaaaaagcag ctaaatttag attttatcgc tgctgaaaat cgtgatactt tttttattga
    12241 cagttttgat ttctttgatg aaaaaatttt tgcttcttct cgtccaggag agaagaattt
    12301 gctttctcct aaaaatttga cgacaaatcc tacatctttg cttgtccgaa cagacaaacg
    12361 ttttgcagga gctgtatctg ctgaattaac acgtcaattt ggcagttatg tcgatgttcg
    12421 gacatggggt ggacctactg ccatcttaga gattgtttca aaaggaatcc aaaaagcaaa
    12481 aggcgtgcaa gagattgcta actacctgtc tatcgatcaa aaagacgtca ttgcctttgg
    12541 tgacgagcat aatgatttgg aattgttgga ttatgcaggt tggggagtag ctatggctaa
    12601 cggtactgac caactcaaag gcattgcaaa tgatgtcact cctttgagca atcaggaaga
    12661 cggtttagct gtttatctgg aaaaactact taaactataa tagagaatta gattccacaa
    12721 aaaagaggtt gtgacaattt ttgtcacaac cttttttgcc gagtaaacgg tgttcaaaag
    12781 ccagtttctc ggtatgaagt caaatttaag caaaaatgtg agaagctatt ttcacaaaat
    12841 ttttcttcat actcgaaact aagcggcttt ttcacgacct cttttttatt ttgccatgaa
    12901 catatcataa tacgcgtcca ttatttcttt agcaatcgtc aagtttatgt ggtcttgttc
    12961 gtctaataag ttcggcaaga caacactgat ggcaatttcc ggattgtctg ttggtccgta
    13021 tgccacgata ttactattga ctgtcgcaat atctggatga ccttccgcaa ccgtttctgc
    13081 agtccctgtt tttgcggaaa ggtccatttt cgcactggct aatggtttgg ctgttgtgta
    13141 aggatcggta ccatggacaa cttggtggaa tccttcacgg aggatcgcca tattttctgc
    13201 agaaatattg accgtatttt ctactgttgt gccaatttct ttcttcactt cacccaagtt
    13261 tccattttcg tcattgccat agatcgcttt gactaggtgc ggctgtactc gtttcccgcc
    13321 attagcgact gttgaagcat attgagctaa ttggattggt gtatatgtgt caaactgtcc
    13381 aaatgccaaa tcggtaaagt tacctggagc ataccattct ttgccattat ccgcattgaa
    13441 cttcttcata taatcaacag aacgtgagat cccaggagat tcattcggca aatcgatccc
    13501 agtcgttgta ccaagaccaa actctttgaa agagtctctc aattgctgat aagctttagc
    13561 ttcttcatct aaactaggta gtcccattcc aggtgtatag tctagtccta acatctttaa
    13621 cgccactttg atcatatacg tattagaaga caattccgat gctttgactg catcaagagc
    13681 aacttgtccg cttcgattaa agacagagct tttttctgaa gcaccttgca gacgaatcgg
    13741 ctcatcaatc aaaacttgat ttccgctgat cgcgttactt gcccagcctg ctgtcaaagt
    13801 accagccttg actacagacc ctggatcaaa ggcgctagta atcgtgccta aagcattctc
    13861 ggtgatttct ttggaacctt gttcatgact gaaaccagtc attgctagca cttctcctgt
    13921 ctgaggattc atagccactg cataagcacc aggcgaatac tgtgctttgc cgttgttgat
    13981 caatgtttga taattccttt tcaaaatttc ttctactttt gattggaatt ctgcattcat
    14041 agataacatt aaattagaac ctttttcccc agagaagatt tctttttgat tcgaaacatt
    14101 tccttcatta tcaagggaaa cttcatattg ggttttggtt ccttgcagga catcttcata
    14161 ttgtttttcc aaataacttt gtcctacacg gtcattccga gaataacctt ttgctaggta
    14221 tttctctgct tcatctgctg gaagtccttg cttttcagtc gtcacactgc caagaatact
    14281 tttcagtgaa cttgctgcat tatattctcg tgtccagtct gtgccagtcg aaactcctgg
    14341 aagttcagaa gcgcgttctg ccacaacagc taactcttga tcagtaacac cagagttttt
    14401 gatatagaca gtgtttaatt catatgcact attcatccgt ttaaagatgg tagcaatttt
    14461 taattgctcc tcgtttaaat taatctcatc agctgtgact ttattgacca tcgcactata
    14521 ttgttcgctg ttccctaatt gcttttcttt tgccgaaagt ctttttgtgg cttctttcaa
    14581 atgtttggga tcagccagcc aaaaatcttt tttatctcta tctgtcagat tttcatctac
    14641 cggaacatcg atcagcgagt tcagcttcgt tgcgatttca agcaaatctt tagccgtaac
    14701 ttgtgttcct ctcgtatatg tgatggcctg attcgcttta ttcgtaacta aagcttttcc
    14761 tgtggagtcg tagatcattc cgcgaggagt actttcttgg accgtaatcg tagaagtaga
    14821 cttcactcgc gcctccatct cttcgccgtt cacgatctgc aaataaccca atctagcaat
    14881 caacgcaaca aataacgtaa atataatgaa aaaaagaaaa tttaagcgaa aaggaacatg
    14941 ggaccggcga aacgttttgt tcttaggttc ttgtgcacct tttcttattt tttctaacca
    15001 agaagtattt aatatttttt tcataaaatc tttcatcatt tcctatcctt ccgcctattt
    15061 acattcatgc ttcattgtac ccaaaaactt cttaataaaa aagaaaaaat gggcggagca
    15121 agaaattctt tacctaattt caagataaaa tcaacttttt gtttcacagc aatcacttaa
    15181 agttacatat ccaactattt ttttcattaa aaaataaact tatttttgaa aattatgttt
    15241 tcttaagtcg tgaaacactt tgatggatca tttatttgtg gaaaactacg ttttgttatt
    15301 ctaaaaaact tatataaaaa atagtaaggt ttttatttcg aatccataaa atattcttgg
    15361 attgcttctc gtgttttttt cactttttcg ctaagctttt cgtttttagc catatagaaa
    15421 taaaaaatga tatcgatcgt agcaaattgg gccaaaagtg aattagttgc cgcacttcga
    15481 ttcgtgattt cttttggtct agatgtttgt acgagtacat cggctatctt gctaagcggg
    15541 ttatttccaa attgcgtcaa cgcaatgatt tcgatattca tgcttttcgc cagctcggct
    15601 aatgcgacta catctgcact tctacctgag tttgaaacaa gccaaagcac tttctttttc
    15661 tcattgctga caagttgcgg gagcaagacg tggatatctt tttcaaaaat gattggctta
    15721 ccgacacgag accatttttg cagaatatct tctactacta aagaagaggc gccaacccca
    15781 tataaaaaaa cagtctctgc cgattccaaa cactctacga ctttttggat cgtttcttct
    15841 tctagaatat ctactgtttc ttttatcgct acttgtgcat tgttaccaag tttgtttttg
    15901 atagagtaaa aggactcatt tgcttctata tcactaaaac cttcatcgat tccagtctgt
    15961 agttctgttg aaatcgtgat tttgaagtcc gtcagacttt gaaaaccgat ttttttggaa
    16021 aaacgtacaa ctgtcggcgc actcaacccg gaagcttctg ctaattcatt agctgtcatt
    16081 gtagggacct tctcaatatt accgataatg tagttggcca actttttttc tgctgaagaa
    16141 taatcttcca tcatactttc aatacgacta attacgctca taagttcctc caccctttta
    16201 atgatagtct atgtaagtat accatatcag acccttattc acggtcgtat actggataaa
    16261 aaacaaaaaa acaatccgcc caacagaata tcttatccca tcaatcccac agattctatt
    16321 gagcatctgt gtctttgagg aatacctgtt atctattgtt cggattgctt tctatctact
    16381 acttttttac aggaaattta cctattagtt ttctctgctg taatttttga cttctgcttc
    16441 catagcatca ataaagatat tcaaatcttc tacagctgtt tctttacttc cataacgacg
    16501 tacgttcact gttgcatctt ccaattcctt gtctccaaca actaattggt aagggatctt
    16561 ttgtgtttga gaggcacgta tcttatagcc cattttttca ttacgatcat caacttctac
    16621 acgcaacccc ttcatttgca aacgttcttt gatttcataa gcataatcgc catgtgcatc
    16681 aacagaaaca gggatgattg ttgcttgtat aggtgccaac catgttggaa aagcgccttt
    16741 gtatacttct gttaaataag cgacaaaacg ttccattgta gaaacaattc cgcggtggat
    16801 aactactgga cgatgcgtat tttccccgtc ttctccaaca tatgtcaaat caaagcgctc
    16861 tggcaataag aagtctaatt gtatagttga taatgtttct tctgtaccaa gagctgtttt
    16921 gacttgaaca tctagttttg gtccgtaaaa ggctgcctcg ccttctgctt caaagtaatc
    16981 tagttctaat tcatccatag cggctttcag cattgcttgg gctttttccc acattgcgtc
    17041 atcatcaaaa tatttatctg tattgtttgg atcgcgatag ctcaagcgga aacgataatc
    17101 tgtaatattg aagtcagcat atactgccac catcaattca agcgtacgtt taaattcatc
    17161 tttgatttga tcaggacgaa caaatgtatg accatcattc aacgtcattt ctcgtacacg
    17221 ttgtaaacct gacaaagcac ctgatttttc atagcgatgc atcattccta attcagcgat
    17281 acgaattggt aattcacgat aagaatggat cgtgtttttg tagaccatca tgtgatgagg
    17341 gcagttcatt gggcgaagaa caagcatttc gccatctccc atatccattg gcgggaacat
    17401 gtcttcttga taatgatccc agtgtccaga tgttttgtac aactcaacat ctcccattat
    17461 tggtgtatat acatgttggt aacctagact aacttctttg tctacgatat aacgttcgat
    17521 cgtgcgtcgg attgttgccc cttttggaag ccagaatggc aaaccagaac ctacttctgg
    17581 ggaaaccatg aacaagtcaa gttcttttcc tagtttacga tggtctcttt cttttgcttc
    17641 ttcacggaga cggatatatt ctttcaaagc tttcttgtca aaaaaggctg tcccgtatac
    17701 acgttgcatc atttgattgt ttgagtttcc acgccaataa gcacctgcca ctgataataa
    17761 tttaaatact tgtatacggc cagtggatgg aacatgtggt ccacgacaca aatcgatgaa
    17821 ttcgccttgt tgataagcag taatcacttc ttcttctggc aattcactaa tcagttctac
    17881 tttgtacgga tcacttgcaa aaatttctaa tgcttcctgt tttgaaagaa cacggcgttc
    17941 gatcggcaga ttttctttga cgattttcat catttctgct tcgattgccg gcaaatcttc
    18001 tgcagttact tggttttgtc cattatctgt atcgtagtaa aaccctgaat cgatagctgg
    18061 tccaacgcca aagtggatat ttggatagag gcgacgcatg gcttgggcca tcaaatgagc
    18121 tgctgaatga cggactaagc caagtgcatc ttcatggtct ggtgtgatga tctcaatact
    18181 tgcatcttcc tcgattggac gagtcaaatc aacgagtttt ccgttcactt taccagctaa
    18241 agcttttttt gctaagcttt ttgaaatgct ttcagcaatt gccaatgttg ttgttccaga
    18301 ctcaaattct ttgactgcac catctggaaa agtaattttt atcataatag tttcctcctt
    18361 gaaatgtttt gattgatcag atcatcaagc tgaaaaacaa aaaaatccta gcatctcttg
    18421 aaaagagata ctaggacgaa aagttcgtgg ttccacctaa tgttcaaacg caaatgcgtt
    18481 cctcaagcgt tgtaacggtc cgaccgccct tgcttcaaca agggtttcga aagtggtcat
    18541 cctttggttt tctcctggaa aagtttcagc ctagcttttc ctctcttatc gaagactctc
    18601 aaattcagtt ggctttctca tcaatcaatt atgtgtttat ttaaccacta ataaatagaa
    18661 aaatcaagtg ggaaactaaa cttatttttt ccattcccca tttacaccgt atgttccttt
    18721 tttcatctca ttgatgatga catggatgtg ttcttttggt gcctgcgtgt ttttatgaac
    18781 agcttccgtg atatctttca tcatattttc taattgttcc ggactgcgac cttctactaa
    18841 ttcgacatgt acgaatggca taatgctctc tcctctttct gatcgttctt ttttcaactc
    18901 gattctatca tactatgttt gtcttgggta ctgaatcagg aaaaatggct ggatcttttt
    18961 ttgctctcaa ggcactcaaa atagagaccg tatccacgac ttcttgcaaa acagctccga
    19021 ataatgcggg gattagtccc gtacttgcaa ttaacatcaa aaaaatacaa ataaagatgc
    19081 caatcagtac agactgtttc gctattctca tcgtatctct tgaaatcact actgcttttg
    19141 ctacacgcaa gagatcatct ttcaagatga ccgcatatgc actttcacta gcagctgtag
    19201 aaccatgagc ccccattgca ataccgatat ctgcagcagc aagtgctggt gcgtcattca
    19261 cgccgtctcc taccatcgca acaggtcttt gatctttagg gatactttct aaaatggtca
    19321 gtttatcttg aggcaagcta cgggcatgta cagaatctaa ttggactttt tctgcaatcg
    19381 tttgagcaat ggcagatcca tccccggtga tcatcatcaa atttttcaca tcaagttttc
    19441 ttagctcctt gattgtcttt gctgcttctg ggcgaatctg atccgaaaat aggatcgttc
    19501 ctgcaaaaga tcgatcaatt gttacataga ttgctgtatt ggacgtttct atttcttcag
    19561 ctgaaacaaa agaggcacgt ccaatcttta cttcatgatt attgactact gcttggattc
    19621 cttgaccggt cacttctttc agttgactaa caggatacag ttggctgttt ttcacgttgt
    19681 tcactaacga acgagctaag atatggctag attcctgttc tgcactagct gcatacagaa
    19741 gtagctcttc tttttttatt ccattgactg gctgaatcgc ctctacttcc aatatccctt
    19801 tggtaagcgt tcctgtttta tcaaaagcaa tcgtttttgt acgtgcgagc ttttcaatcg
    19861 ttgttccagt ttttacaacg atcccgtttt tacttgaacg gctcattcct gcaactaaag
    19921 caatgggcgc agctagaatc agtggacaag gtgatgccac taccagtacc tcagcaaatc
    19981 gtaaagggtc ttttgagaac caccaagaaa taccaccgat caaataagca atcaacgtga
    20041 aagggactgc ataacgatca gctaaacgga caaatcgggc aggcttttct tttgattctt
    20101 cgactagttt aataatcatt tgatactggc tgtctgcggc acgtttttca gcgatcattt
    20161 ggatcgatcc gtctccattt aaagaccctg acataatctg atctccaggt ttcttggttt
    20221 ccgggcggga ctcacctgtc aaagaagatt catcgaccga agattctcct ttggtcactc
    20281 gaccatctgc gggtacaagt tcgccaggct tgactagtaa ctggtcatca atatttattt
    20341 catcgattac tatatcctgc aattttcctt ctttgaatcg atgggcaatt cttggggtgt
    20401 tatctaacaa agactgcagt tctttacccg ctcgtttatt cgcgtaatcc tctagactat
    20461 ctccgcccgt aagcatgatc aatatcatca aactagccca gtattctttg acggctaatg
    20521 ttgcaaggat tgcggtaatc gctaatacat ctacgccata tcttcctgaa cgtaacgtct
    20581 tgatcatttc caaaaacatc aagaaggcga gaatccctcc agtaaatgcg acaatcgcaa
    20641 atgcaagggc cggctggtga aaaacaaatt cacttaataa agctatccca ccaataagca
    20701 aagtaagtaa aaattttttg aaatgtgtca ttgttttttc tcctctttta tgtgacttag
    20761 agaggttagt aataaatttc cagctgcctc tttcatctta agcatagtcc ttgactagca
    20821 ctttttaaaa caataggcct taattgaaaa caactatcac tatcagcgtt tccaaaattc
    20881 atcagttcta caaacaaaaa tattcagcaa ggattacttt ttcgattcat cagttaaaaa
    20941 gtaccttttt tcgaaaaact atgctatact tttaattcgt tatttacggc ggaaggagca
    21001 aacatgcgat gaatgagaaa caactagaac aaacgcacgt ggatgaaacg atcgaactaa
    21061 tccagatcga acaaaatatg ctggatacaa aacaacaaga attgactcag gaaatgaatc
    21121 attcgacaaa agacacagct aatcacaaga ttcgagctgg ttccaatgaa gctttttatg
    21181 aatctgctgt tgaatatcgc caacatgagc aggaattgct tttgaaatac catactcttg
    21241 aagcacagca aaaacgcctg caaacgttgt ctgtcatgaa agatagtcct tattttgccc
    21301 gtatcgactt taaagaagac cgtgaaacag aaacattata tctaggtatc gcttctttaa
    21361 gagatctggc agaagaaaca atcgttattg actggcgcgc accaattgcc aatctttatt
    21421 atgaaggtga gattggtccc gcattttatg aaactgaaac agatagaata gatgtcgatc
    21481 tgcagttgaa acgacaattt aaaatccaag aaggacgtat tttatctatg gtagatacgt
    21541 cggaagtaat caatgatgac tttttgctag agatattgga tgaatcatca agcaaccata
    21601 tgaaaaacat cgtctctacg atacaaaaag cacaaaatgc aatcattcgg gacacaacaa
    21661 gtaaagtaat gctgattgaa ggaatcgccg gaagtggaaa gacatccgct ttgcttcaac
    21721 gtattgcttt tctgttgtat cataatcgca agtggctcga ggctgaaaac gttcttctat
    21781 tttcacccaa ccatttgttt tctgattaca tctcgactgt attgccttca ttaggtgaaa
    21841 gcggcgtgcc tacacagaca ttcaaaaatt atttggtgca gctgctgcca gagttttctt
    21901 tactagatga acagcaacaa gaaagtggtt ttttgctagg ggaaaaagat ccgatacaag
    21961 tgatgaaaag cggattgacc ttagtcgatc agattgatcg ctatatccaa ggcatcacaa
    22021 actacggtcc cttgttccgt gatatgaaaa taaatggccg tacgatccta tcaaaggaat
    22081 cgattcgcca atggtataaa gaaacaaatg aacaattacc tttacatcat agactttctc
    22141 tattacaaac gaagctcctg aaaaaattag gcggtctgca aaaagacgaa acacgtcagc
    22201 aatgggtcaa agatctggcg gaagaacagc tccaagaact atacgcaaca gatccaaatc
    22261 ttgaatatac agaacaaaag gagcgttcat tacgtaaaaa actggccaac caaatcgtac
    22321 gtaaaagatt ccgtcgaatc catcgaggga tcatgaatta tcaatttgtc aatataacga
    22381 aacaatatct ccactttcta caaactgtgc cactttctct tctaacacga cataaaatca
    22441 gcgagaccag gtggcaggaa catttgacaa ctgttcgtac acacttgcgt caaagagaat
    22501 tgcgtcaaga ggatgctgta ctattctttt tactgatgcg ccgaacatat ccagtgacgg
    22561 ttacacacaa agctcgctat atctttattg atgaaatgca ggatttcgca ccatcacaag
    22621 tggcattgct gcaagccttg ttcccaaaag caaatttgac tttctgtgga gatctaaatc
    22681 aaaatgtctt tggaaatgaa acgatcgttg gatcattgga agaccttttc gtggaccgtg
    22741 aagtcactcg tttccaatta acgacaagct atcgctccac aaaagaaatc actgattttg
    22801 ctaatcattt tattgtgaaa gaacatcaag tagaaacaac agcaagaaaa ggagaccttc
    22861 ctttgctcat ccaaggaaca acagatgata tcaaattgaa ttggttagca gataccattc
    22921 gaacgactca gggaaaagcc cggtattggc gtacagcaat catagctaaa acacaagaag
    22981 aatgcgaagc tctttatgct caactgccag acgagttgaa acaacaagtc cagctgattt
    23041 taaatgagag tgactttatg aagcgttcca ttattttact tccagccttt ttagcaaaag
    23101 gtctggagtt tgaccgagtt ttcttgtggg atgtagatgc agaacatttt tcttctcagc
    23161 aagacaaatt gatcctttac accatgtgta cacgtgcgat gcatgaattg acccttttta
    23221 cctccaataa tgaaagtcct ttgttaaaca atatggatat ttcatcttat gagaaaaaag
    23281 tactgtaagt aaaaagacaa ctgctatcat taatgttagc agttgtcttt ttatttacaa
    23341 taataattgc agaaaaagta attcaatcat ttaaggtcat ttattctata tataaatcca
    23401 aatctgtttc aggatatttt aatttgacaa gcgaaataat cattttcagt tcttccatgt
    23461 tttctggata ttctgggtca aataccttgc tatgacggcc aatcccgcca ttgtcgatca
    23521 atcggtaaat tcgattccat tcaatgaatt ggtcaattga ttggaaattg ctgattggat
    23581 agccgccaca atccaaaatc tctgatacac gaaatgctag gtatgcacta tcgatcactt
    23641 tatttttttt cgtgtctatc ttctcatttc tttcgataag aattttttca ggacgatgtt
    23701 tcaagctcca ctctaatagt agaaagaaag cctccatccg atactcttct tcaaataacc
    23761 gaaaggctga atcagaacga cgaaaaagca gcggaatcag ataattttgt ctataaatca
    23821 gcattggtgc gcggtccacc atacactcta aataacggtt cagataccct gtaccgacat
    23881 agtttcctaa tcgatagcct tcaacgccga atcttgcgta tttttgcgct aattttgaac
    23941 aatcagttac gttcacagct aactcttcta ctttgcggta accgcttaag atacgttcaa
    24001 aaggttctaa agtgattttt gcttccatat tcttccccgt ccatcttcca catacttcat
    24061 atcatttcac atgatactta tgctctcatc ataccacagg ttgaattaga aaagaaaata
    24121 aatcacacaa aaaagcaaga caatctgtta gtgagattgt cttgcttttc gaaaaagact
    24181 gaatactttt cgttaaaact ggttgcttag tcttctaatg ccacattatg gaacacatgc
    24241 tgtacatctt ctagatcttc cagtgcatca atcatttttt caaatgtttc ttgatcttct
    24301 ggagatagtg ttacctcgtt ttgagggatc atttggatct cagcgattga aaaatcagtg
    24361 attccttttt cttttaatgc ttcttgtata ctatggaaat ccgctacttc accgtaaacg
    24421 atgatttgtc cttcttcttc cattacatcg cgaacatcga tgtctttttc cattaggtat
    24481 tcaaggatct cgtcagcgtc ttctcctgca aaagcaaaga ttgctgtatt gtcaaacatg
    24541 taagaaaccg ctccggaaac acccatgttc ccgccatttt tcccaaatgc tgcccgtacg
    24601 tctgctgctg ttcgattcac attgttcgtc aatgtatcaa caatcaccat tgagccgctt
    24661 ggtccaaatc cttcatagcg caattcagag tatgtttcat ctcctgcacc ttttgctttt
    24721 tcgattgctc ggtcaataat atgttttggt acattataag ttttagcgcg atcaatgacg
    24781 aaacgtagtt tttggtttgc atgcggatca ggatcacctg attttgctgc cgcgtagatt
    24841 tcaatcccaa attttgcata aatccggcta ttattagcat ctttagcagt tttctttgct
    24901 acgatatttg cccatttacg tcccatattt tcacttcgct ttctgatttg atagtttcat
    24961 aacgcaaaaa cgtcctgttc cattatactt gtgaatcacg attctgtgaa gctttttttc
    25021 tttcttcctt cattcttctc atttttagtc aattatctat actcggattg ggatttgccc
    25081 gaaattctgt tccggaatat tgcctcccaa ttgatagatt tgatcacttc taactaacag
    25141 ttccgcttct tgtgcctctt tttttccgat tcgggaataa atagaaatct cttttttttc
    25201 ttttaagaat cttcttccag catcggtaaa tccaagtagt cgaagataat ttttattttg
    25261 ttggacagga atctcttgct ttttgatatt cagcaagaca taacaagata atcgttggat
    25321 ccgtgtccat gtatatcttt ttgtttttac ctttgacaca aaatcctcaa aactatcagc
    25381 agttgcagct gctgttttca tgcgcgattc tattccttcg gtcatctgat agatatctgt
    25441 cagttccagc aaagtagaag ataacaaccg ataacgcaaa tacggccaat aagattccca
    25501 agtctgcagc gaacaagttg aaagatcttc ttctgtctga ggcggcacaa gtccagctat
    25561 tctttttcct tcttctaacg catggcgaat ggctgttgca ctggctatcc tctgatctgt
    25621 gatgattttt tcatgatagc ctgcactttt acgtcggatc ggatgtaatt ccatagggtg
    25681 gacatacttg gcattttcct ttgcataact taatcccaag atatgatttg gcgaagaaaa
    25741 atcaagcgta atatcgggat acagactgcg aaaaacctca gtcatttttt gcggataact
    25801 atacgtttga tcagacagtt cttgaaatgc ctcttctatc ctatcttgat tctcttggac
    25861 aaagcgtccg aaactgctgt aatcaaacga agtctcttct tctgttccaa aacacaaact
    25921 gtcacagtcc agtgtttgca agatttttac tgcaccttta gcaaaatagt cagctgactg
    25981 aaccgcccat tcgacgggaa gctccactac taaatccaca ccattttgta aagcttcatt
    26041 cgcgcgatgc catttatcga tgatagccgg ttcgcctctt tgcaaaaaat tcccgctcat
    26101 tacagcaatg acgacctctg ctcctgttat ttttcgtgcc atttccacat gatagcgatg
    26161 tccattgtga aaaggattgt actctacgat aatcccgcag cttttcatgc gcatcctcct
    26221 tcatagttat cgataaaaaa gaaatagaga caagtaaaaa aaggcaaaaa tcggaaaaaa
    26281 tacccgaact atataaacta ttctcgcaaa cacaatactt ctgcgacgaa aagctgcact
    26341 gcaagagcaa tgcctatatc gttcggatat ataatttatg ttgaagattt attcttttct
    26401 tgttaactcg agcaggcaaa accgctagtc tttgtatgct acaaagaacc agcgctttga
    26461 tttctctgat gctgcttcgt ctgtaaaatc agaaaatgct tcgactttcg aaaagccgct
    26521 gttttccagc attcttaaat aactttccaa tggataggta cgctcttgat gtagttcatc
    26581 ttcacgaata aatcgaccag acccttcttg ttcttcaacg aaaaaggtaa gaaaatgttc
    26641 gatgctgtgt ttctcgcttc ctggataact atcccacaaa aaagcaaaat tatccccttg
    26701 ataatgatag ctatattctg gaaaaacctc atctacttga taggtggagt gtacgtcaaa
    26761 taaaaatcgg ccattttctt ctaacgcttg atagacttca tcaaacactt gctgtacctc
    26821 ttgtctattt ttcatatagc acagcgaatc agaaaaacag gtaattgcct gatattgtcc
    26881 gatttctgat aaatcgagca tattgccttc cacaaattgg atcgatgcgt cttcttcata
    26941 tgctcgctgg ctggcaatca tcaacatctc ttctgaaagg tctaaccctg tcactttata
    27001 tccatctttt gcaaaggcaa ccgctaatgc tccagtaccg catgccagtt ccaaaatatt
    27061 ttttgtgtct tcttgtaaat gacgtttcga aaaagctaac cattgctgat aaagtgtttc
    27121 gtccatcact tcatcgtaaa tgaaagcaaa tgtttcataa gccatatgtt aatccaacca
    27181 ttcactaaga tcgactagcg gagcatctga ccatagtttt tccagattat agaaatttcg
    27241 ttctggcgca tggaaaacat gtacgatcac atctccaaga tcgatcaata cccattttcc
    27301 gccttctttt ccttcaatac gttttacttc atatttgttt tcttcttctt tatcaatgat
    27361 ttcttctgta attgcgttga tttgacgttc gctatttgct gaacagatca tgaaataatc
    27421 tgccaacaag gaaacatttc gaacgtctaa agcgataatg tcttctgccc gttttgaatc
    27481 tgctgcttca atcgcaattt ttaattgttc tttactatct atgatgattt cctcctaata
    27541 tgagcagctt atttttttgc tacccagtaa ttatatgttt cgattgtttt tggataaatc
    27601 tgttgttctt gcgcaagcaa atgggccaat gtatgttttg tttcaaatgc tacagcttcg
    27661 tctagatcag cccatgcaat ggctcgtgca tcttgtaccc caggaaaatc tcgacctggt
    27721 tcaatatagt ctgctacata aatgattttg tcaaggagac tcatcttagc agccccagtc
    27781 gtatgcaccc ggattgcatg aagaatctct gcatcggtga ttcctaattc ccgctctaca
    27841 aacgaagcgc caacaagacc atgccagatt gcattattat aattcaataa ctccggatca
    27901 aaaccgtctc ttacgattac cattttgaac tcctcatctg gtcgttcttt tgcataatca
    27961 tgtgtcaatg cagcaatact agctttttca ggagaagcac cgtatttttt ggcaagagcc
    28021 acggctgttt cttctactcc aagcacatgc ttgaagcgtt gttcgctcat tctcatctgt
    28081 attttttgca tcaggttttc tctagaataa gcagtatatt cattactata gtccatattc
    28141 atacaaccct tcattttgaa tataatcaat tactttatct ggaacaaggt agcggataga
    28201 acagccttct ttgatctttt gacgtatttt cgtggaactg atgtcgatct caggtacatc
    28261 tacccaaatt acaggatatg gagtatccgt tgtgtatcct ggtcgacgta tacccacaaa
    28321 gttgaccata gaagtcagct catcgatttt ataccatttt ggcaaatatt ctaccatatc
    28381 cccaccgata ataaagtaat aatctgtatc cggattgttt tgcgtcaatt ccttcatagt
    28441 atcataggtg taacttttcc caccacgagc taattcaatc gtttcaatct gcaaaaaagg
    28501 attgtcttcc accgctaatt ccaacatgtt gagtcgatgt tttgcatcaa tcgtctgttt
    28561 ctcatcaaca tgaggtggct gataagaagg catcaaaaat acgcggtcaa gacctaaatt
    28621 ccggccagct tgttcagcca tcactaaatg tgcaaggtga acgggattga atgttccgcc
    28681 caagatccct acttgctttt tcttctctaa gaattgaggc atttcttcag gaaataattg
    28741 tgaacgaaca aatgtcttag cttgcgtatt catcatggtc actccttata ttgcttttac
    28801 ttcttttgat agcctttgat acttttcttt tgtggaaggt ttgtataata caataacacg
    28861 tccaatgatt tggacgattt cgcaatctat ttctttttct aatacttgag ctgcttcttc
    28921 tgcaatctca tctgtatttt gaagtaacga tactttgatc agttctcttt tttctaaagc
    28981 ttcttcaatt tgaacgatca ttgctgaatt gatcccacct tttccgattt gaaaaatcgg
    29041 ctgaagatga tgcgctttgc tacgtaaaaa acgcttttgt tttcctctta aactcataat
    29101 tttccttctt ccttaaatca atgctttgcg tttcaataca tctacaccct ttggcgccca
    29161 gcccgcaatt actactggcg ctgttacagt gatccagcct agaccggcaa aaacgatgtc
    29221 tgttttttct ttcacagaaa actcaaaacg aacaagctcc ggaaatgcct cgacttcatc
    29281 aggacgagga ggctgtaata atccacctac atgtttttga taaaattcgt ctgctttttc
    29341 cagtttcgtt cgatgaatat ttacatcatt tgacacataa gcaataaatg aagcacgatt
    29401 gccgctgata aaatcaaatc ttgcaagtcc gcccaagaaa agtgtttgcc cttcattcag
    29461 ctgataaact ttaggtttga tttcttttaa cggtgcaatg atccgcaaat cttttttacc
    29521 taagtagtgc gccatttgat ggcgatggat aattcccggt gtatcaatca aaaaatgccc
    29581 gtcatctaaa ggaatctcga ttttatccaa ggtcgttcct ggaaactgcg aagttgtgat
    29641 tacatcttgt actcccgctg tatttttgat gatttgattg ataagcgtcg attttccgac
    29701 atttgttact ccgacgatat agacatcccg tccatcgcgg tattcttcga ttttttctaa
    29761 tagttccgtc atctcatgag atttcttagc actagttaat aatacatcta ctggacgaat
    29821 tcctgcttcg taagcacgtt ccttcatcca ttgtgttaat ttagtttttt tcaatgattt
    29881 aggtaatata tccactttgt tcccaactaa aagtactgga ttgtcaccga caaaacggtg
    29941 tagtcctggg attagtgaac cattgaagtc aaaaatatcc accacattga cgatcaatgc
    30001 atctttttgc cctaattcat tcagtaagcg caaaaagtcg tcatccgtca atgagacatc
    30061 ttgggtctca ttataatggc gtaatctgaa acaacgctga caatagactt ccccggaagc
    30121 taaacctttt tccaaagccg tttgcggcgt gtatcctaat tcaccgggtt tgtttgtttg
    30181 tataacggca ccacagccga tacattgtaa ttcttctgtc attcgattcc acctcgccag
    30241 cccatatcag ggtgttttgc taatagatag gccataattc ttttttccca aaaacgatta
    30301 aattgtgtct tccaactatc tgttgcgata atgggtttta caaggatgct acgtatccct
    30361 gcacgattgg ctccacgtat atcagtcata atctgatcgc ctaccattac tatctcatca
    30421 tctgctaaat ccaattgttt ctttgctatg ttgatccctc ttgctaaagg tttcattgct
    30481 cgtgcaacat acaaaagatc aaatttttca atcgctcgcg cgacacgttt tgaattattg
    30541 tttgaaacaa caacaacagg gattcctgca tttttcattt ctaaaatcca agtaagcaac
    30601 tcttctgttc catctggatt attccatgca atcaacgtat tgtctaaatc ggtcaatact
    30661 gctttgatgc ctagattctt taattgggca ggtgtaatct gatagatggc ttctaccatc
    30721 catgttggtt tgtaatttga aaacatgctg tctcctcatc tgcaaaaaat gaaggagcac
    30781 ataaagtact cctcaacccc gtattatact gcatatcagc aaaaaaaact acctctatac
    30841 tcgttcgtct ttcagaaaaa gcatttcttt acaaaatgac caaaaataaa gattgatcac
    30901 agaaaaattt agaaacaaaa aacaactaat attggcggaa cctcttgtat cgtttttcca
    30961 gtaatttatg ctgatctaat cgaacaagtt ccgtcaatct ttcaatcaac gcttttttca
    31021 taatgtgtgc gactttatat cgtggcaact gctcgccgtt gaacgtttca ggaataactc
    31081 gatcaacgat tcctaactcg cgtagttctg gcgcagtaat tttcatcaac tcagctgctt
    31141 ctgcggcgcg actaccgtct ttccacaaaa tcgacgcaaa tccttctgga gaaagcacag
    31201 cataaatcgt ttcttccagc atccagactt catccgctaa acctagagca agggcaccgc
    31261 cacttccacc ttcaccaatg atgatggcga tgatcggtac ctttaaatca gacatttcga
    31321 gcagatttct ggcaatagcc tctccttcgc cgcgctcttc tgctcctaca ccgcaatatg
    31381 cacctgctgt attgatcaag gtaatcaccg gtctattgaa cttttccgct tgtttcatca
    31441 gtcgtaaagc ttttcgatag ccttcaggat ggggagaacc aaaatttctg cggatatttt
    31501 cctgcagatc ccgtcctttt tgaataccga tcactgttac cggtttgcca tctaatcgag
    31561 cgattccacc aacaatcgct tcgtcatctg caaataaacg atcaccatgg cattcctcga
    31621 aagaatcgaa gatctcttca aaaaaatcca agcttgtcca tcgttcttta gctcttgcta
    31681 actgaacgac atcatgtgct gtcttatcca cgatctatcc atcctttctg tgtatgcaat
    31741 tcaagaagcg tatggatttt ctgtttcaat tccatgcggg gaacaatctg atcgacaaaa
    31801 ccatgagata ataagaattc agccttttgg aaatcttctg gaagttcctg cttgatcgtc
    31861 tgttcgatga cgcgtcttcc agcaaaaccg atcaagcttt ggggttctgc tagaataata
    31921 tcgccttcca ttgcaaaact ggcagttact cctccggtcg tcgggtccgt taaaacagtt
    31981 agataaaaaa gaccttcatt gctatgccgt ttgacagcag cagagatttt tgccatctgc
    32041 attagagaaa agattccttc ttgcatccgt gcgccacctg aagctgtgaa caagacgact
    32101 ggaagacttt cctttgttgc tcgttcgaat aatctggtga tcttttctcc gacaatcgtt
    32161 cccatacttc ccatgataaa attaggatcc atcactccaa taatgaaggg aatcccctga
    32221 atggttgctt taccagtgag gactgcttca tgaagtcctg ttttatcttg cattttttta
    32281 attttttccg tatattcggg aaatgctagc ggatctttgg ttacaagatc cgtatcccat
    32341 tcttcaaaac ttttttcatc aataatcaaa gctagacgct gccatgcacc gattcgaaaa
    32401 gcgtatccgc aatgagggca tactttttct tcaccgatgt ctttcgtata aaggatatgt
    32461 ttgcaatttg gacactttgc ccacatgtta tcaggaacag atggcgagtt agcaggtttg
    32521 ctctctctat taggattgat ccgaatatat ttctttttct taaacagagc catctatgcg
    32581 cctcctatct ccgtttctgg tgtccaattc ggtaaaaatt cttcttgtaa aaatgctgtg
    32641 ctgtaatcac ctgctatgac tgcaggatga ctaatcaaat ccatctgaaa ttctgcgttc
    32701 gtgatgattc cttctgtgat aaattcactt aaggctcgct gcatcttcat taatgcttca
    32761 aaacgcgtat ttccatggac gattattttt gcaatcattg aatcataata tggcgggatc
    32821 gtatatcccg ggtaaactgc actgtctact cgcagtccca tcccacctgc tggaagcagt
    32881 aagttttgga tcttccctgg agaaggagca aaatggaaag caggattttc tgcattaatc
    32941 cggcattcga ttgcgtgtcc ttggaatact acttcttctt gacacacatt caaaggctca
    33001 ccggctgcta tctccacctg tttttttact aaatcgattc ctgtgaccat ttctgttact
    33061 ggatgttcca cttggattcg agtattcatt tccatgaaat aaaaatcgcc ttcttcatcc
    33121 atcagaaact cgatcgttcc ggcattttca taattgactg ctttagctgc tcgaacagca
    33181 gcgtctccta gtgcttctct ttttgtttgt gagatcacta cagaaggaga ttcttccagt
    33241 actttttgat tgttgcgttg taaagaacaa tctcgctcgc ctaaatgaat gacatgacca
    33301 aatcgatcac ctaagatctg aacttctata tggcgagcag gataaatgat tttttccaaa
    33361 tacatatcat catttccaaa tgccgctgct gcttcttgct gagctgaaga aaaatgctgt
    33421 gggagctctt cttttgattg aactttccga atgcctttcc cgccaccacc tgcagctgct
    33481 ttcagcatca ctggataacc aattctgtct gctatttcca atgcttcttc tactgtagat
    33541 aatacaccag tacttcctgg aatgactggg acatttgctt cttgcatcaa ttgacgcgca
    33601 ttgatcttat ttcccattgc atcgatcgtt gcacttttag gaccaataaa tgtgatattg
    33661 cactcttcac acatggaggc aaatcggcta ttttcagata aaaaaccaaa tcctggatga
    33721 attgcttcgg cttttgtcac gattgcagcg ctcagtactt cctgaacatt cagatatgaa
    33781 tcaacggctt ttgccgggcc aatgcagatt gcttcatctg ctaactgtgt atgaagtgct
    33841 tcggcatctg cttctgaata tatcgctacg gtttggatcc ctagttcgcg gcatgcacga
    33901 atgatacgta cagcgatttc accgcggtta gctatcaata ctttagaaaa catatttcac
    33961 ctatccaatc ataaaggtta attccgcttc ggctactttt ttaccgtcta ctttagcgat
    34021 tcctttgcct attccggctg aagctttcac tttcaatatc tcaacttcaa ggtataaggt
    34081 atctcccggt gtaactttct tacggaattt tgctttgtcc aatccgccga aataagccgt
    34141 ctttcccttg aattgttcaa gagaaagtaa tgcaactgct cctgcttgag ccattgcttc
    34201 aacaatcaat acccctggca taacaggttc ttctggaaaa tgtccttgaa aaaagggctc
    34261 attgatcgtt acatttttct tagcaacgat ccgttctcct tcaaccattt cttccactcg
    34321 atcaatcaaa agcattggat aacgatgtgg aatgatttct tttatttctt gtatgttcat
    34381 tgttttccct ctttcctgct ttgcttattt ttgctgaatc ctaaagagtg gctgtccata
    34441 ttcgacaact gactcgtttt ctacaaggat ttctgtgatc actccgtcgg cctcactagt
    34501 tatctcattc atcagtttca tcgcttcaat aatgcagata acttctcctt tggcaacttg
    34561 atcacctact tttttgaaag ctggtttgtc gggcgctggt tgcagataaa cgattcccac
    34621 gattggcgaa acaacctcga ttccatcaaa tactgccgat tctttttctt tcattatttc
    34681 tggagcagtt gttgcttgtt caggtatgac attgactggc tccgaaactg gcaacgtttc
    34741 gtttgtttga accatattca tgcctcgact tgttttattt ttattcatat acaattcaaa
    34801 ttgaccttca cgcaaatcga attccgttag cgaggattga tcaaattgac tgactaattc
    34861 tctgatttca ttgatatcca aaattattcc tcccagcgtt tcatgcacag aaccgcatta
    34921 tgtccgccaa accctaatga attactcatt gcataagtaa aacttgcagg tttgctttcg
    34981 tttttgacaa ctgtcaagtc aatctcagga tcttgttttt ctacattgat cgtaggtggg
    35041 ataaattgat gttctaatgc cagcaaagtt gccaaggctt cgatcccgcc ggctgctccc
    35101 aataaatgtc cagtcatcga tttcgtgctg ctgacgtaag tatctctgaa attttcacct
    35161 aatgcgattt ggattgcttt tgcttctgcc tggtcattgg ctggtgtact tgttccgtga
    35221 gcattgatat atccgacttg ttccggctgt acaccagctt cttccatcgc aagcttcatt
    35281 gctttagatg caccagaacc atctggagta ggagctgtca tatggtaagc gtcacagttt
    35341 gcaccgtatc caacgatttc tccatagatt ttggcaccgc gagcttgtgc atgttctaaa
    35401 gattcaagaa ctagaacgcc tgctccttca cccatcacaa aaccattccg attttcgtca
    35461 aacggaatcg aaccacgatt aggatcagtt gattctgaga gtgctgttaa agctgcaaat
    35521 ccagcaatcc ccatctcaca aatcgttgct tctgttcctc cagcaagaat gacatcagaa
    35581 tagccatgtt tgatgttacg gaatgcttcc ccaattgaat tcgttccgct agcacaagct
    35641 gttacgatag ctgtacaaat accttttgca cctacacgca aagcaatatt tccagctgcc
    35701 atgttggcaa tcgccattgg tacgaacatt ggagaaactc gttttgcacc tttttcatgt
    35761 agacgaataa cttggttttc gattgttgga agaccaccga tcccagatcc gatgatgacg
    35821 ccaaaccggt cttgattcat tttttcttta tctaaaccgc tcatctccat tgcttctaaa
    35881 gcagcgtaga ttccatagat cgaaaacata tccattcgtt tgctatcttt tttaataaaa
    35941 tatttatcgt aagggaaatc tttgacttca cccgctaagg taatccctgt ttctgaagca
    36001 tcaaatttag tgatcggtgc gatgccattc gtcccctctt ttaagctatt caaaaagctg
    36061 tctgcgtcat ttccaatagg tgaagtgact ccatagccag taattaccac tcgattcatt
    36121 tgtttgctct ccttttctta tccgtgcatg accaatccgc catcgacatt gatgacttgt
    36181 ccggtgatat atggattttg tgctaagaaa actgctgttt gtgcaatatc ttctacttgt
    36241 ccgaaacgct gcatcggaat ttgcttttcc gcttgttcct tgactttgtc tgctaaaact
    36301 tccgtcatat cagttgtgat gaatcctggc gcaatggcat tacatgtaat gcctctagtt
    36361 gcagcttctc gtgccacaga ttttgttaat cctactacgc cagctttact agctgcataa
    36421 tttgcttgac caatatttcc aatcaaccca gatacactag agagattaat gatggcccct
    36481 tctcgctgct tcatcatttt tttcaatacg tgctgcgtca tattgaaggt tccaatcaaa
    36541 ttgatatcca gacatttttt gaaatcttct gcatccattc gcatcacaag tttgtcattt
    36601 gtgattccgg cattgtttac taacacatgg atcgatccaa gcttttcttc tgcttctttg
    36661 atcatttgtc ccgctttctc ataatcagag atgtcaccgg aaactcctac gcattttaca
    36721 ccgaatgctt cgatttcttc tatcttttct tttggaatct ctccgcgacc attcaaaatg
    36781 atatttgctc cagcttttgc aaaagctaag gccatcgctt tgccgattcc tcttgtacta
    36841 cctgtgataa atacattttt tccttttaca tccataacgt tcctccttat agtattgcta
    36901 ttgtttcatc aaatgtctgc ttattctcaa cacgcagaac tggtattgtt ttatcgattt
    36961 ttttcataaa tccgcttaat acttttccgg ggccgacttc gatgacttgt tcgatgccga
    37021 ttgtttttaa cttatggata ctctcataga aacgaacagg ttgcatgact tgctgttcaa
    37081 gtagtcccgc gattgtttct ttcttcatta tttccgttgt tgtgttgctt atgataggaa
    37141 aagaaggttc agagaattga atctgactta ggtccttcgc cagttttttt gctgccggtt
    37201 ctaaaatagc tgtatgaaaa ggcccgctga catttagagg gatcattctt ttgaaccctt
    37261 tttcttttaa tagtaaaaca gcttcgtcta ctgctttttc ttcgccacca atcacgatct
    37321 gctgaggtgt attataattt gcaggagata cgatgccgta cttgctagct tcatgacagc
    37381 tttcctcgat cgtttcaatc ggcgcattca tcactgcaac cattttcccg cttccagctg
    37441 gggcagcttc tgtcatataa gcgcctcttt ttgctactaa cgcaactgct tcagtgaagg
    37501 atagcgcatt actagctact aatgccgaat attctcctaa actcagtcct aaagctgcat
    37561 ctgggatgat tccatgctcc ttcaataagc gataatacgc gatgctgact gttagaatag
    37621 ctggctgtgt atattgcgtc tgatccaaac gttcattttc tgtaaagcaa agttcagcca
    37681 tatcgtagcc gagaatctca cttgcttcat caaatgtctg ctttacaacg gcttcttcat
    37741 ataaatcttt tcccatacct tgatattgtg caccttggcc actaaataag aatgctgttt
    37801 tcatgttttt ctccaatata tccagaatat atgtttgatt cttcctattc aaaaaggccg
    37861 agacattcct atttcaggaa atgtcccagc ccccaaccta attttaatga ttatgcagtt
    37921 tgtttttcta cgtaagtcac taaatcttga accgttttga ttccgtcttc tgtttcgatt
    37981 ttcacgtcaa attcatcttc aatttcattg atgatttgga ataaatccaa gctgtcagct
    38041 tctaagtctt cttggatgtc agtagtcaat ttgatttctt cagtttcttt tcctaattct
    38101 tctgcgatga tttcttgaat tttttcgaat accataataa tatattcctc cgatattgtt
    38161 tgctttttat ttttataatt taacagtcta caactccatg agagtacttc cccatgttaa
    38221 acctccgcca tagcctgtaa atattacttt ctgctgtttt cctaaggtga tctttccact
    38281 agaaactgct tcatccaata agatagggat gcttgcagca gatgtattcc catatttatc
    38341 catattggtc aggaactttt cttgcggcat ctttactttt cgggcaattt tctcaataat
    38401 tctgaggttt gcctgatgca aaagcagata atcaactggt ttgtctttca tgacttcttg
    38461 gatattgttt gcaacgtctc gtacggcaaa atcaaaaata tctctgccag tcatttgcaa
    38521 ataatacgaa ccttctgaat cttctgaata gaacggattt ttattttgct ggtagccagc
    38581 ggtcaatgag ctacttctgg taccgtcagc tgctaatttt tctttcagca attttttctg
    38641 tggattcgct tctaagagaa tcccacctgc tccatcgcca aacaacacag cagtcgtacg
    38701 atcagaccaa tcgattagct tagatagtgt ttcaccacca atcaccagtc ccttagttga
    38761 gccgcagcgg atcatttttt cagccatgct taacgcataa acaaatcctg agcacgcagc
    38821 actgatatca aaagcgatag catgagttgc tcccaatttc ccttgtactt gtgctgctac
    38881 agatggtgtc gtgtaatcgg gcgacattgt tgctactaaa ataaaatcca aatctttagg
    38941 gtttagattt ttctgtttta tcaatttttc cgccactaaa atgcataaat cagaagtctc
    39001 ctgatttgtt acacatcttc gttcacgaat acccgtcctt gacgaaatcc attcatcaga
    39061 ggtatccatc atcttggaaa gatcattatt agtaataatc ttttcaggaa ggacgctggc
    39121 tgttgcagta atcattccaa attgttccat taaatatcct cacttatagt cttgcaagaa
    39181 gtcgtgcaag tttttcagtg catccagcaa ggcatgctgt tcctccgtag ccattccgtc
    39241 taagatgttc ttgaccattt cacgatgaaa atgctgatgg acccgaaata gtaactttcc
    39301 ttttttcgtt agtcccaact ttacaacgcg gcggtcatct tcactgcgga tacgttcgac
    39361 atagcctttt tttactaact tgttaatagc agtggtcaag gtcccaacag taattgatag
    39421 ttctttggct acttctgaag ttgttttttt cttgtacatc ccgattgcct cgatcgtgtg
    39481 catctctgtg attgacagat ctttgaattg agattttttc agctcagact cttcgattgt
    39541 caaaatatca ttgaagacac taacaagata atcatttact gtttttaaat ctggttccat
    39601 gttccaccgt ccactaatta gtttgattat caaatctttt gatgttcaaa ctatatctta
    39661 acttttttca aaatgcaacc ttaaaagatc atttttttac tatatgagct ttttttaaga
    39721 ttttactcat ttaaaacaac tgataaatca gcatttataa tttcttttat tagaaaaatc
    39781 atctatctca catattttga ttatcaaagt attttctgtg aacagaacta tagaattccc
    39841 taaaacttgt ccattatttc aaaatttgtt atagtctttt tattcaaagc gtaaaaggag
    39901 atcctatgtt aaaagaaaaa atgaaaactc tccccatgct gccgggtgtc tatcttatga
    39961 aagaccgaga tggtaaggtc atctatgttg gtaaagcaaa acggctcaaa aatagagtaa
    40021 gcagctattt tcatcagaat aaacagcatt caaaaaaagt attacgtatg attcatcata
    40081 taacagattt tgatttcgtc gtagtagaca cggaattaga tgctttattg ctcgagtgtc
    40141 aattgatcca acattaccgt cctttttata accgacaaat gaattatttc agtaattata
    40201 attatgtaca tattaccaat aagggatttg tattaacaga tactcctacg gctcgaacat
    40261 acgggccttt tcgtctctat aaaaaaatgc cgtctatttt gcgtataatg gaagaaactt
    40321 atcaaatgcc ttggttgtct gaaattagtt tgttagcttt gcgagtccag cttcctgacc
    40381 tacaggaaat gacttttgaa cagaaaaaaa aggaactcca agggctgttt caaggccgaa
    40441 ataaaaagct actgacttac ttgaaaaaaa gacaacaaca tttcatctat cagttgaatt
    40501 tcgaaaaagc cggtatgctt caaaaagata tcgaattagt tacttatttt attagacgga
    40561 tccaggaaca aaagcagttt ttaagaacac catcgctcac cttcagcatg ccattagcag
    40621 cagatgagag tcagaaaaag cactacctcg tttgttatgg tcaacttgca gaaacaatga
    40681 ttacctctgg tgacgtttct cctgacttct attatgaaaa gaaagaagct catttatctt
    40741 tgaaacgtca attgtcaaaa gaagaaatcg atcctgtaca aatcttgatc agttaccgca
    40801 aaaaattaga aaaagaacag tttgaaattg aactgttaaa caaaaaagaa gctgaaaagc
    40861 aactaaatta gttgcttctc ggcttctttt aggcatcttc aacttcaaat gtcaattatc
    40921 ctaaaattta ttggattaag tcatagattt ccatcgctac gatatcgata ttatcaaatt
    40981 cgtattgctg atgagtatct gtttcagtta attcaaatgt atcggtcgct ttatcataag
    41041 ttactgtaca cttctctacg ccttcttttt caaaacggcg aacttgcagt tcattatctg
    41101 ctgactcgac catagcctcc agtcgtttta tgattgcgac taattgtgaa tgtgtcatcg
    41161 gaggtcctcc ttttcttatt tctgttatat tgtatcaaaa gcatagccta cttgcattga
    41221 ttttctctaa aaaaactcgt tttttatgat tttttttctg gtatttttcc ttcaggccac
    41281 caaagtttga tcaaagcttg ggttccgtca ttctcaaaac ctttatctgc caactcttca
    41341 tatagtttca atgcttgggt agttgctggc aatacaagat ccattttttc tgcttcttct
    41401 aatgcaatct ttaaatcttt gataaaatgt ttgacaaaaa aaccaggtgt gtaatcttct
    41461 tttaaaatac gaggagaata attgcttaaa gaccagttag ctgcacttcc acctgaaagc
    41521 gtctcaatca ctttctttaa atcaagtccg tttttttgtg catagacaag catttccgtc
    41581 attcctgtca tcgtacctgc aatcatgatc tgattagcca tttttgtatg ctgacctttc
    41641 ccagcagaac catgcaatgt aaatgtcgta ccaaattctt tgaataaagg cagtacttga
    41701 tcataaacgg cttcttctcc accgaccatg atcgtcagtg taccattctt tgctcctaag
    41761 tcaccaccgg ataccggtgc atctaacgca tctgcgcctt tttcttttgc tgtttttgca
    41821 attttttctg ccaggcttgg tgtacttgta gtcaaatcaa ctaaaatatg tcctgagata
    41881 tctgcagaaa aaatccccga ctgtccatag tagatttctt ctacatcttt ggggtaacct
    41941 accattgtaa agagaatatt actttgttct gctaccgctt ttggtgtatc tgcccagata
    42001 gctccttctg ttactaaagg atcagctttt gcttttgtcc gattatagac agctacttcg
    42061 tgtcctgctt ctagtagatg gcgtgcgaca gcacttccca tgacgcctgt tccgataaaa
    42121 ccaagtttca ttttttcgac ctcctaaaaa tcaaaaatcc ttgcttctat gctagtatac
    42181 gctagaaaca aggatttttt cataaaaaat gtttgaattt tatcttttaa atgaatttaa
    42241 ttttattttg attcgttcaa tcgagtcttc ttcttctttt cttcgcatct cttcttcatg
    42301 ataaacaacc atgtcttgat aaaattcgat catagcttcg ccgaaatgtg tagatgaaat
    42361 atcataaagt ttagcttcta aaacttttgg atctttagga acttcactgg aaatataatc
    42421 aataagtgct ggtgcaaagt cttcgtcggt ttcaaaagtt ttccctaatg aaggatgatc
    42481 gaacaatcgg ttcaaataag catttccttc cacgacacaa ggtgttccgg ctgccatcgc
    42541 ttctgtataa gtaagccctt gtgtttctga agtagaagca ctgacaaaat aatctgccgc
    42601 tttataataa agtgctacat cattgttgtc cacttctcct gtgaactgca caaattcttc
    42661 taactgcagt tctgagatca attcctctaa ctcttctaca taaggtcctt ttcctacgat
    42721 aactaatctg gcgttaggat aagcggagat gacctgagga aacccattaa tcaaggcttg
    42781 gatatttttc tcataagaaa tcctgcttaa agaaagaatc atcaaatggt tttcctgaag
    42841 acctagtgac tcccttaatt tgctgatatc ctgctgagtg atatcagaac gtttgaattt
    42901 ttcaatgtcg ataccagtag gaatcacgcg aagcggtgct attacaccat agctgcgtaa
    42961 tgtatcaata acacgttcac ttggacaaac tacacctgtc gtatgatttg caaataatct
    43021 agacaagtat tttacatggg attgcctgat cacttttcct ttaccgatat aatgaaggta
    43081 gtcttcatac atcgtatggt aagtatggat cagaggtatt ttcaacttct tagctaccat
    43141 cttcccaagt atccctgctc cgaattctgt atgggtatga ataagatcta gttctagttc
    43201 tttggcaacc aaataagcat accacatgcc tctaacaaca actcttcgat ccttgaaaga
    43261 aataaatggt acacttggca ttcgaatcac atctttttcc aacttatccg cattaggatc
    43321 ggtcgttgta aaaatataga cttcatgccc atgtctttct aattcttgtt ttaatgtttt
    43381 gatcgatgta gcaactccgc tgacttgcgg aaagtaggta tcggtaaaaa agccgatttt
    43441 catgtttttc gcctccaaac tagtccttga cctgatttac tttttttaat ttgctagaga
    43501 ctttttcatt cagtacactt tcgtatacat ttttaagttc ttcaccgatt ttattgatac
    43561 ttcgctcttt tgctgtttga taaccggctt gtgtcgtact cggtagttct tgattggtaa
    43621 tctgttggat cagtcggata aattcttcgt ttgtcttccc catatagcag ttcttgctat
    43681 cttctaacca gccattatac actggaatat ctcgaacaag tacgttttgt ttgcttgcta
    43741 aagcttctaa aacaacgatc ccttctgtct cttcataaga agggaaaaag aatagatctg
    43801 cacctgaata cgcaccttct atgatctctc cacgaatgta accaggaaac aggacatttt
    43861 caggatgatc ttcttttaca attttccgga tgttccgagg aatcgagtac atgggaacat
    43921 gaccaaacca aataaatgtg tattcgggca tcttcttagc aatttctacg aaatcaatga
    43981 ttcctttacg ttcgaaaaac aaaccgacac aaatgattac tttttgatcc ggagacagtt
    44041 tgaaatattc tctgaacttt tgttctttca ttggatcagg gcgatatttc tctaagtcga
    44101 tcccattgga aatagcttga atcggcacac ggatactata gctttccaac aactgttttg
    44161 aataaggtgt tggcgtgatc agatagtccg ctttttggta caatcctacc agatattttt
    44221 ttacgatagg agaaagctgg ttcgatccga taaaggaatt tcggaaatct tcctctgtag
    44281 aatgcgcatg atagacaact ttttttcctt ctcttctggc tttattgacc atattatgac
    44341 tattgattcc gtatgtattg atgtgcagga tgtcataatc ttctttttca tctagtgtat
    44401 aagagatacc gacttccgtc agagcacgtt tttgatgatc aagcgcacgc ccgattccag
    44461 attttgcaag catcttttca ctttcaaaat ataataatac cttcacctta cgtccccctt
    44521 cacttatata gtataacata gcctacaaac tgtcactcaa acttaagccc tatttttatc
    44581 tattcttact gacaaaaacg ttttcttaaa aaagaacaaa aaaactggcg tagctctttt
    44641 acaagccaca ccagcttttg atacgttgtt tattttacat attcatgaac aagttcaacg
    44701 acttcttcca tcgtatcgca ttctttcaat gcacgatcag caagttcagc cattttcgct
    44761 gtgtccaagc gtttcattaa actacgcgtt ttaagaatag atgtcgcact cattgagaac
    44821 tcatctagac ccatacctac tagcaatgga acagctgttt gatctccagc catttctcca
    44881 cacatacctg cccattttcc ttcagcgtgt gctgcatcaa ttacattttt gatcaaacgt
    44941 aaaattgatg ggttatatgg ttggtacaag taagaaacgc gttcgttcat acggtctgct
    45001 gccattgtgt attgaatcaa atcgttcgta cctacagaga agaaatctac ttcttttgca
    45061 aacttgtctg ctaagactgc tgctgctgga atctcgatca tgatgccgac ttggatcgta
    45121 tcagatactt ccttgccttc actgatcaat ttttgttttt cttcttcaaa gatcgctttt
    45181 gctgcgcgga attctttgag tgtagctacc atagggaaca tgatacgtaa gttaccatga
    45241 acagatgcac gaagcaatgc acgcatttgt gtacggaaca tttcgtcgcc ttgttcagat
    45301 aaactgatac gcaatgcacg atatcctaag aatggattca tttcatgagg taattgcaga
    45361 tatggtagtt ctttatctcc accgatgtcc attgtacgaa caactacagg ttttccttcc
    45421 ataccttcta atacagcttt gtaagcttca tattggtcat cttctgttgg gaagtctgga
    45481 gaatccatat acaggaattc agtacggtac agaccaactg cttctccacc attattatgg
    45541 acaccaacta gatcttttgg tgttccgatg tttgcagcta attcaaaatg cttgccgtca
    45601 gctgtgacag tttctgcatt ttttaatttt tcccattctg ctttttgtgc tgcgtaatct
    45661 gcacctgctt tttcaaattc agctttttgt tcatctgtag gatcgatgat tacatcgcct
    45721 tcgatcccgt tgacagccaa aatatcgcct tctttgactt tagcagtaat ttcttttgta
    45781 ccaacgattg ctggaatttc taaagaacgt gccatgatag cagaatgtga agtacgtcca
    45841 ccaatgtctg ttacaaacgc ttttacaaaa ttgcgatcta attgtgctgt atcacttgga
    45901 gtcaagtcat gtgcaacaac gaccacttct tcgttgatca ttgatggatt tggtaaagta
    45961 acgcctaata agtgcgctaa aatacgttta gcaacatcac gaatatctgc agcacgctct
    46021 tgcatataag cattatcttc cattgcttcg aacataccga tatacatatc cgttacttct
    46081 tttagcgctg attcagcatt tacgctgttg tctttaatat tttgcttgat ttgacctacc
    46141 atttctggat ctgaaagaac catcaaatgg gcatcaaata cttgcgcttc tgcttcacct
    46201 aagctttgcg ctgctttttc acgaatttgc tgcaattctt cagtagattt agctaatgca
    46261 gcatccaaac gagcttcttc agccgcagta tcttctactg ttgccttact gaatgttaaa
    46321 tccggttgaa ctagcaggta agcttttgca acggctacac cgtcactagc ggcaatccct
    46381 tttagcattt caaccattat tcagataagc cttctttctt cattgtttca acgattgctg
    46441 ccattgcatc agcttcgtca gcaccttctg cagtaatagt aacatcagaa ccttgaccta
    46501 cacctaaaga cataacgccc atgattgatt tcaagtttac tgatttacct ttatactcta
    46561 agtttacatc agagttaaat ttgcttgctg tttgtactaa tagtgtagct ggacgagcgt
    46621 ggatcccagt ttctgctact acgtgaaatt ctttcttttc catattaatc ggtctccttt
    46681 aagtgagtgt tgtttttatg tgagggtttt cttttttagt aattcagaga atcctaacaa
    46741 atgccaccct ttcatctgta aagattaaca ttttttttcg ttagatacaa cagttttcat
    46801 tcaatttcag ataaaagtat tcaaattatt ccggattctt tttcatcctt ttcttcacat
    46861 tttataacaa acgtgtttat tttctttttc ttgtcgcttt ctcttctcct gacgtttatc
    46921 attctttcct ttgaccctct ttgttttcat atgaaacgaa ttacttgctt ccctttttat
    46981 ttttagtata ataagagtca aaggtcagga atggtcaata gagatttcac cttttctacc
    47041 tcataaatct cttaaaaaac taggggttat tcttttacct catgaaaaac gagatgtaaa
    47101 atatggatgt acccattttt gtgaaaggac gtgaatgatt catgctttgt caaaactgcg
    47161 gcaagaatga ggcaacgatt cacttgtatg ccactgtcaa tggtcagcgg acacaactcg
    47221 attactgtca gagctgttat caaaaaatca aaaatcaaca aaatggaggt ctgactaata
    47281 tggcccaaaa tgatccattt ggatttggca gtttggacga tcttttccgt tccatgtctc
    47341 gccaaatgca acagcaagga aattatgagc aaacaccacc aacacaattt ggcggaaaca
    47401 acaacttcaa tggtggtcag ccacctcaag gcaatacaga aggtttattg ggagaatacg
    47461 gaatcaatat cacagaacaa gcacgacaag gtgatatcga tcctgtgatc ggacgcgatg
    47521 acgaaatcaa acgagtcatt gaaattttaa atcgccgaac caagaacaat cctgtcttga
    47581 ttggagaacc aggtgttggt aaaacagcag ttgttgaagg tttagcacaa aaaatcgtag
    47641 atggtgatgt accgcaaaaa ttaatggaca aagaagtcat tcgtttagat gtcgtatctt
    47701 tagtccaagg tactggtata cgaggacaat ttgaagaacg tatgcaaaaa ttgattgaag
    47761 agatccgtca agcagaaaat gtgatcctat tcatagatga agttcacgaa atcgtcggtg
    47821 ctggttctgc tggtgatgga aatatggatg ctggtaatat tttaaaacca gctttagctc
    47881 gaggagaatt gcaaatggtt ggtgctacta ccttaaatga gtaccgcatc atcgaaaaag
    47941 acgctgcttt agaacgtcgt atgcaacctg tacgggtaga tgaaccaact gtcgaagaaa
    48001 cgatcagtat tttgaaaggt ttgcaaaaac gttatgaaga ctatcatcat gtaaaatata
    48061 ctgatgaggc catcgaagca gccgcaactt tatccaatcg ttacgtccaa gatcgcttct
    48121 tgccggataa agccatcgat ttgctagatg aaacaggttc taagaaaaac ttgacgatcc
    48181 aaatcgttga tcctaaaaca atcgagaaaa aactgcaaga agcagaagaa caaaaagttt
    48241 tagcttctcg agaagaagat ttcgaaaaag cagcttatta tcgcgatcag atcaataaac
    48301 ttcaaaaaat gaaagaacgt cagctcactg aagaggaaac acctgtgatt accgaaaaag
    48361 atatggaaaa aatcgttgaa caacggacag gtatttcagt gggtgaatta aaagaaaaag
    48421 aacagacaca actgaaaaac ttagcagatg acttgaaagc tcatgtcatc ggacaagata
    48481 acgctgtaga tcgagtagcg aaagcgattc gccgaaaccg tgttggatta aacaaacaga
    48541 atcgtccaat cggctccttc ttatttgttg gaccaactgg tgtaggtaaa acagagcttg
    48601 cgaaacaact tgcctacgaa ctattcggtt cacaagattc aatgattcgt ttcgatatgt
    48661 ctgaatatat ggaaaaacat agtgtctcta aattgattgg ttctcctcca ggttatgtcg
    48721 gatacgaaga agctggtcaa ttaacagaaa aagtacggag aaatccttac agtcttgtct
    48781 tgttagatga agtagaaaaa gctcatccag atgttttgca tatgttcttg caaatcttag
    48841 atgatggtcg attgacagat gcacaaggaa gaacggtcag ctttaaagat acgatcatca
    48901 ttatgacaag taatgccgga actggtaaag tagaagcaaa cgttggtttt ggtgcggcac
    48961 gcgaaggtgt tactcgttct gtattgaacc aattaaacaa ctactttaca ccagaatttc
    49021 tgaaccgttt tgacgggatc atcgaattca gtgcattatc aaaagaaaac ttgatgacaa
    49081 tcgtcacact aatgctagac gatgtcaacc aaatgctggc agcacagcaa cttcacatcg
    49141 aagttcctac gaatgtcaaa gaaaaattag ttgacttagg ctatgatcca tctatgggtg
    49201 ctcgtcctct tcgacgcacg atccaagaac aaatcgaaga tggtatcgct gaattttatc
    49261 ttgatcaccc ttctatccat gagttgaaag caaaactgga taaagatgga aaaatcatcg
    49321 ttacttctaa accagaacgt ttggctaaag aatcagccga agaaacggct gaataaatcc
    49381 agttttatag cgtgagacgg tatttctttg agaaatgccg tctcattctt ttttcagaaa
    49441 agtcaaaata ctgttcattt tataaaaaag aaattataat aagagaaaca agcaattaaa
    49501 aatacataaa agaggtgagc gccatgaaat tggtaaatgt aacaaacagc cattcccgtc
    49561 ttgtattaaa ccagcttgaa aacactgatg cccatctagt caaggtctac acagccggaa
    49621 ataccactat tgtctacact gaagcccctg aacataatga aattctgctg atcaatgaca
    49681 aacgtaaaat ccagccgaaa gaaatcgaag atgctaaaaa ttatttctta gataaaatca
    49741 aagacgccgt ttaccacgag gatgaaatca aagtaatcga attggcaggc cttgttgaaa
    49801 tctcgattcc aaaacatgaa ttaggcgcta cattagccgt gtaaacaaat tttgaacata
    49861 ataat
//
LOCUS       DACGLE010000013        49312 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0534
            SAMEA3304449-rid9560453.denovo.014, whole genome shotgun sequence.
ACCESSION   DACGLE010000013 DACGLE010000000
VERSION     DACGLE010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304449
            Sequence Read Archive: ERR985064
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 49312)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49312)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702225.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702225.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 20:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,045
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 27 of 67
            Pseudo Genes (incomplete)         :: 25 of 67
            Pseudo Genes (internal stop)      :: 19 of 67
            Pseudo Genes (multiple problems)  :: 4 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49312
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304449-rid9560453.denovo.014"
                     /strain="VREN0534"
                     /db_xref="taxon:1352"
     gene            complement(85..1011)
                     /locus_tag="IXT20_000869"
     CDS             complement(85..1011)
                     /locus_tag="IXT20_000869"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-dependent inorganic pyrophosphatase"
                     /protein_id="HAQ4859336.1"
                     /translation="MSKILVFGHQNPDTDAIGAAIAFSHLQNELGKETEAVALGTPNE
                     ETQYALDHFQLSAPRVVESAKAENADVMLVDHNEFQQSISDISEVNILAVVDHHRIAN
                     FETANPLYYRAEPVGCTSTIILKLYKENQVTIPKEIAGIMLSAIISDTLLFKSPTCTP
                     EDEAAAKELAAIAEVDLNTYGLEMLKAGTNLSDKSAAVLLDLDAKSFPMGSSNVRIAQ
                     VNTVDLNEVMDRQAELEAAMEDEMKANGYDLFVLVVTNILDSDSELLVAGKGKEKVEE
                     AFQTTLTNNRALLKGVVSRKKQVVPQLTAVFS"
     gene            complement(1096..1857)
                     /gene="pflA"
                     /locus_tag="IXT20_000870"
     CDS             complement(1096..1857)
                     /gene="pflA"
                     /locus_tag="IXT20_000870"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase-activating protein"
                     /protein_id="HAQ4859337.1"
                     /translation="MEEKTIGYVHSIETFGSVDGPGLRFVVFMQGCRMRCQFCHNPDT
                     WNIGGGKEYTADELLDKAERFRPYWGDKGGITVSGGEPLLQIDFLIELFKKAKERKMH
                     TTLDTCGKPFTYEEPFFSRFQELMKYTDLLLFDIKHIDNEEHKKLTHWDNENILEMAQ
                     YLSKINKPVWIRHVLVPERSDYDEYLIRLDNFIQTLSNVDRVEILPYHTMGKYKWETL
                     GLKYPLEGIEPPTKERVENAKRLLHTEEYTKYLTR"
     gene            complement(2101..4332)
                     /gene="pflB"
                     /locus_tag="IXT20_000871"
     CDS             complement(2101..4332)
                     /gene="pflB"
                     /locus_tag="IXT20_000871"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HAQ4859338.1"
                     /translation="MEQWNGFKGTKWKEGVDTRDFIQNNYTEYRGDDSFLEGPTEATE
                     KLWDELQKLNDIQFERNGVYDMDTEIVSTITSHDAGYLDKDLEKIVGLQTDKPLKQAF
                     MPFGGINMANNALKSNGYTPNDELTHIFTDWRKTHNQGVFDAYTAEMRAARKNKIITG
                     LPDAYGRGRIIGDYRRVALYGIDYLMEQKKKDHDMTGYHTMSEEVIRLREEITEQYRA
                     LAQMKEMAAKYGYDISKPAANAKEAVQWLYFGYLAAIKSQNGAAMSIGRISAFLDIYI
                     QRDLDNGVITEKEAQELIDHLVMKLRMVKFARTPEYNQLFSGFPIWATLSIAGMGLDG
                     RSLVTKNDFRLLHTLTNMGPSPEPNLTVLYSEHLPEGFRTYASRIAIESSSIQFENDN
                     QLREQWGSDDCAIACCVSGTVVGKDMQFFGARANLAKTLLYAINGGVDEVTGAQVGPE
                     YRPITSEILDFEEVKHNFMNMMDWLAELYVNTLNIIHYMHDKYSYEAAQLALMDSDLQ
                     RTFATGIAGISHAADSLSAIKYAKVKAIRNEKGIAVDFEVDGDFPKYGNDDDRADELV
                     SWILEYFMGQIKRHKTYRNSKPTTSLLTITSNVVYGKNTGNTPDGREAGKPLAPGANP
                     SYNAEQSGLLASLNSTAKMNYGCALDGISNTQTINPSGLGKEQDTRIENLRNVLDGYF
                     SKGAYHLNVNVFSNDLLRDAMENPMKYPNLTIRVSGYAVKFRDLTREQQLDVLMRTSH
                     DRM"
     gene            complement(4609..7059)
                     /gene="parC"
                     /locus_tag="IXT20_000872"
     CDS             complement(4609..7059)
                     /gene="parC"
                     /locus_tag="IXT20_000872"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HAQ4859339.1"
                     /translation="MENRQEVQELTLEEVMGDRFGRYSKYIIQERALPDIRDGLKPVQ
                     RRILFAMNKDGNTYDKGFRKSAKSVGNIMGNYHPHGDISIYEAMVRMSQDWKLREVLI
                     EMHGNNGSMDGDPPAAMRYTEARLSKLSGEMLADIEKETVDLVWNFDDTEKEPTVLPA
                     RYPNLLVNGSTGISAGYATEIPTHNLAEVIDGTIYMIDHPQASLEKLMEYIPGPDFPT
                     GGILQGKDEIKKAYETGRGKVILRSKTKIEAMKGNKQQIVITEIPYEVNKATLVKKMD
                     EIRLNKKIDGIAEVRDESDRTGLQIVVELKKDANAQGILNYLFKNTELQINYNFNMVA
                     IDHMTPHQVGLKDILRSYIEHRKQVITKRSQFDLAKAQKRQHIVEGLMKALSILDEVI
                     ATIRESKDKKDAKKNLVDVFQFTEEQAEAIVTLQLYRLTNTDITELQKESESLIAQIT
                     ELNKILSNDKELFSVMKKELREVKKNYSSARLTTIEDEIEEIKIDTQVLVAQEDVIVS
                     VTREGYVKRTSLRSYSASKPEEIGMREGDYLLYSGELSTLDHVLLITNKANVIYRPVH
                     ELPDLKWKDAGEHISQTISNLSVDESILAVFPYQKIQAEKTFVFISKNGLIKQTRMTE
                     FEPWRTYKSRPLSCMKLKAEEDELIAVYLKEDQPGLDVFLVTHQGMGLRYPLTDVPVV
                     GAKAAGVKSMNLKDGDRIINGLLVLAEGDTPIVIVTQRGAVKRMLAQEISQTSRAKRG
                     VTVMRELKKNPHRIIYMSEGRSKEITLMNQKGQQLTIDPTDFPIGDRTSNGSFAMDEK
                     KGGEVIEVIDAPEIFIES"
     gene            complement(7071..9116)
                     /gene="parE"
                     /locus_tag="IXT20_000873"
     CDS             complement(7071..9116)
                     /gene="parE"
                     /locus_tag="IXT20_000873"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HAQ4859340.1"
                     /translation="MAKKVNNEYNDSSIQVLEGLEAVRKRPGMYIGSTDSRGLHHLVY
                     EIVDNAVDEALSGYGSEIDVTIHEDNSITVADSGRGMPVGMHASGIPTVEVIFTVLHA
                     GGKFGQGGYKTSGGLHGVGASVVNALSKWLIVTIVRDGVEYQQKFKNGGKPDGTLKKI
                     GKTKKANGTTVHFLPDDTIFSTTKFSYEILAERLRESAFLLKGVKISLSDLRGEEPVK
                     EIFHYEEGIKEFVDYLNEEKDTLTPVVYFSGEKEGIEVEVAYQYNDGYSENVLSFVNN
                     VRTKDGGTHEAGMKAAMTKSYNEYARKVGLLKERDKNLEGSDFREGLAAVLSIRVPEN
                     LLQFEGQTKEKLGTPVARTVVDNVISEQMGFYLQENSEMSQMLVRKAIKAREAREAAR
                     KAREESRNGKKRKKGESLLSGKLTPAQSRNPKKNELYLVEGDSAGGSAKQGRDRKFQA
                     ILPLRGKVINTEKAKMQDILKNEEINTMIYTIGAGVGPEFSIEDCNYDKVIIMTDADT
                     DGAHIQVLLLTFFYRYMKPLIEAGKVYIALPPLYKVSKGQGKKQVIEYAWTDDELAAM
                     IKKVGKGYMLQRYKGLGEMNAEQLWETTMDPTSRTLIRVRIDDAAQAERRVTTLMGDK
                     VEPRRKWIENHVQFTLEEDGSILDKKEDTEISPSVSNDLLDEERADKNENNQLFEVE"
     gene            complement(9282..9716)
                     /locus_tag="IXT20_000874"
     CDS             complement(9282..9716)
                     /locus_tag="IXT20_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HAQ4859341.1"
                     /translation="MSVKNPTQETIFNYLEEAKNIAVVGLSNKPERTSYMVAQVLQDN
                     GYKIIPVNPLLAGEEILGEKVYANLTEIDDPIDIVDVFRRSEFLPEIAGDFLKTDAKV
                     FWAQLGIENKEAAEMLRKAGRTDIVMDRCIKIELGKLKENQK"
     gene            9843..10481
                     /gene="plsY"
                     /locus_tag="IXT20_000875"
     CDS             9843..10481
                     /gene="plsY"
                     /locus_tag="IXT20_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HAQ4859342.1"
                     /translation="MKLILLLILAYLLGSIPSGVWIGKLFFKKDIRQHGSGNTGTTNT
                     FRVLGKTAGIVVLLMDILKGTLATCLPMIFHLSGINPLWFGVCAILGHTFPIFAGFKG
                     GKAVATSAGMLLGFNPVFFVYSCIIFIVSLFCTSMVSLSSMISAVLITLSTIVLPYVA
                     PVILAKPNWLLTIIAFLVSSFIFYRHKDNIKRIRNHTESRIPFGLNSSKKKS"
     gene            complement(10613..11488)
                     /locus_tag="IXT20_000876"
     CDS             complement(10613..11488)
                     /locus_tag="IXT20_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HAQ4859343.1"
                     /translation="MTVIIQNDQLIAEIAEHGAELISLKSKEHGLEYIWQGDPAYWGR
                     HAPVLFPFVGRLKDDQYVYQGKTYQMGQHGFARDMDFEVIEHGQEMASFILRSTPETL
                     EKYPFDFELVISYELGGDGITVHYQVANTGEKEMFFSIGGHPAFNVPLEEGLSFEDFY
                     LAFSPLKSRLRLPLKGPFIDMDQKTLGQTNTNLALNRELFEQDALVFETRGLNAYAIR
                     SEKSSHSVTLSYKDMPYVGIWSPYPKEAPFVCIEPWCGIADTVNSSGNLMEKVGINQL
                     GAHEIFKTKYSITVK"
     gene            complement(11570..12361)
                     /gene="codY"
                     /locus_tag="IXT20_000877"
     CDS             complement(11570..12361)
                     /gene="codY"
                     /locus_tag="IXT20_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HAQ4859344.1"
                     /translation="MTDLLTKTRKINTLLQQKNTFDMQADLPYDKMAVTLGGILDSNT
                     YIIGSDGVLLGYNERHDVNNERIKTMFVEKQFPESYTDTVAQLMKTEANIPITSDITA
                     FPVESREKYPFGLTTVVPIFGAGERLGTIILSRMEQSFADDDLVLAEYGATVVGMQIL
                     YQKSRSIEADVRSATAVQMAINTLSYSELKAVQAIFEALDGDEGRLTASNIADSIGIT
                     RSVIVNALRKLESAGIIESRSLGMKGTYLKVLNSRFKDELAKHSY"
     gene            complement(12379..13779)
                     /gene="hslU"
                     /locus_tag="IXT20_000878"
     CDS             complement(12379..13779)
                     /gene="hslU"
                     /locus_tag="IXT20_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HAQ4859345.1"
                     /translation="MYELKQTPRQIVSELDEYIVGQHAAKKSVAVALRNRYRRLQLDE
                     KMQQDITPKNMLMIGPTGVGKTEIARRLAKIVNAPFVKVEATKFTEVGYVGRDVESMV
                     RDLVENAIQIVEKEQYSRVYTSAKKNAEKRLVKILVPGIKKEQKQSTNQFEQMMNLFN
                     ASQQNTAEPQEEVTEDIRINRRTVLEQLQKGLLDSREVTIEVEDQKKPAPMMNNGLEQ
                     MGIDLNETLGALTPKKKVERTVTVKEALELLIKEESAKLVKDADIHSEAIKLAEESGI
                     IFIDEIDKITSKSQQSGEVSREGVQRDILPIVEGSQVNTKYGIIQTDHVLFIASGAFH
                     LSKPSDLIPELQGRFPIRVELDDLTAEDFVKILTEPNNALVKQYVALIGTENVSVTFT
                     KEAIERIAQIAFDVNRDTDNIGARRLHTILEKLLEDLLFEAPDMQMGEITITEAYVDD
                     KLGSIAANEDLSRYIL"
     gene            complement(13793..14341)
                     /gene="hslV"
                     /locus_tag="IXT20_000879"
     CDS             complement(13793..14341)
                     /gene="hslV"
                     /locus_tag="IXT20_000879"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase proteolytic subunit"
                     /protein_id="HAQ4859346.1"
                     /translation="MVESPFHSTTICAVEKDGKFAMAGDGQVTMGESVVMKGTAKKVR
                     RIYNGEVVVGFAGSVADAFTLEEKFEGKLNEYNGNLQRAAVELAQEWRTQQSMQKLEA
                     MLIVMNDKEMLLVSGTGEVITPDDGILAIGSGGNFALAAARAMKNYAQNDMSAKEIAE
                     NALNIAADICIFTNHNIIVEEL"
     gene            complement(14378..15283)
                     /gene="xerC"
                     /locus_tag="IXT20_000880"
     CDS             complement(14378..15283)
                     /gene="xerC"
                     /locus_tag="IXT20_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HAQ4859347.1"
                     /translation="MTEATWEQKFLRYLIVERGYSEKTKEAYEEDILHFKHFLEESGD
                     ADLLKAEYFTVRVYLSALADHRPSYSRNSISRKIASLRSFYQYLLKEEVIKENPFSYV
                     HLKKKNLRLPRFFYENEMQALFDSVAGDSPLDLRNRALLEVLYGSGIRLSECSSLSVS
                     DIDFDSEVMLIHGKGNKERYAPLGSFAQDALQEYFREGRQVLMTKYHEEHDIVFVNHH
                     GKPITPTGIEYVLNQMIKKSSLDSDIHPHMLRHTFATHLLNNGADLRTVQELLGHANL
                     STTQIYAHVTKESLQKNYRSFHPRA"
     gene            complement(15481..16857)
                     /gene="trmFO"
                     /locus_tag="IXT20_000881"
     CDS             complement(15481..16857)
                     /gene="trmFO"
                     /locus_tag="IXT20_000881"
                     /EC_number="2.1.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FADH(2)-oxidizing
                     methylenetetrahydrofolate--tRNA-(uracil(54)-C(5))-
                     methyltransferase TrmFO"
                     /protein_id="HAQ4859348.1"
                     /translation="MEITKKTSKNNRKEVLSLKMVNVVGAGLAGSEAAWQIAQAGVPV
                     RLYEMRPVKSTEAHHTSNFAELVCSNSLRGNSLANAVGLLKEEMRRLNSVVITSADET
                     AVPAGGALAVDRDSFSETITRKVKEHPLVTVINEEITEIPEGITVVATGPLTSHPLAE
                     SIKAFNGSDGFYFYDAAAPIVDKATIDMDKVYLKSRYDKGEAAYLNCPMTEEEFKAFH
                     EALINAEVAELKSFEKEKYFEGCMPIEVMAQRGFKTMLFGPMKPVGLEDPKTGKRPYA
                     VIQLRQDNAAASLYNIVGFQTHLKWGEQKRVFRMIPGLENAEFVRYGVMHRNSFMNSP
                     ELLKPTYQSTKREDLFFAGQMTGVEGYVESAASGLLAGINAARLAKEMEPVEFPQETA
                     IGSMAYYITHAEGKHFQPMNANYGLFPELPERIRDKKARYEAIAQRALKANEAIKTEL
                     EKTAIPNE"
     gene            complement(16826..18904)
                     /gene="topA"
                     /locus_tag="IXT20_000882"
     CDS             complement(16826..18904)
                     /gene="topA"
                     /locus_tag="IXT20_000882"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002380330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HAQ4859349.1"
                     /translation="MAYKYLVIVESPAKAKTIEKYLGRNYKVMASVGHIRDLPKSKMG
                     IDFENNYEPHYISIRGKGDVIKSLKAAAKKAQKVYLASDPDREGEAIAWHLAYLLGLD
                     LKEKNRVVFNEITKDAVKAAFKEPRTIDVDLVDAQQARRTLDRIVGYSISPILWRKVK
                     KGLSAGRVQSVALKIIIDREKEIREFVPEEYWSIDGNFKKARKKFKANFWGIDGKKKK
                     LPDAQSVKEVTARIDGTDFEVKKVEKKERKRNPANPFTTSSLQQEAARKLNFRTRKTM
                     MVAQQLYEGITLGKQGTVGLITYMRTDSTRIADTAKAEAAAFIEDTYGKEFSRHDHRK
                     IKNAQGAQDAHEAVRPTSVLRTPDELKQYLDKDQLKLYTLIWSRFVASQMTPAILDTM
                     KVTLEQNGVIFIANGSKIKFKGFMQVYVEGRDDGKEEKENILPELEEGDVVQSVDIEP
                     KQHFTQPPARFSEATLIRALEENGVGRPSTYAPTLDTIQRRYYVKLTQKRFEPTELGE
                     IVNSLICEFFPQIVDIHFTAEMEGDLDKIEEGTEAWVKVVDRFYKPFEKELTNAEEKI
                     EKIQIKDEPAGFDCDVCGHPMVIKLGKYGKFYACSNFPDCRNTKPIVKEIGVTCPVCH
                     EGQVIERKSKKNRLFYGCSRFPECEFTSWDKPVGRNCPKCDGYLVEKKVKGGKQVVCV
                     NGDYEEDIQK"
     gene            complement(19026..19883)
                     /gene="dprA"
                     /locus_tag="IXT20_000883"
     CDS             complement(19026..19883)
                     /gene="dprA"
                     /locus_tag="IXT20_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HAQ4859350.1"
                     /translation="MYQIEENLLKLAYTKGISSQGKWALANWMMQYPYKEIGFEDIIK
                     IGKITKHRELFRKSWQEIHANWGKIQSKQSFITCFDPKYPPQLLHLTYPPIVLFYNGD
                     LSLLSCNMLSVVGGRHTSALAKKTVYYLLKPVIEAGYVIVSGCAKGIDTYGHQAAISH
                     GGRTIAVIGTGIEQVYPAENRDLQEVIANDHLLLSEYPPQSGPKKHCFPMRNRIIAAL
                     SKGTCVFEAKKKSGSLITAQQALDLGRTVFSVPGNILTEHSTGCHHLIQDGAVCTVSG
                     EDILAEFKE"
     gene            complement(19939..20706)
                     /locus_tag="IXT20_000884"
     CDS             complement(19939..20706)
                     /locus_tag="IXT20_000884"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HAQ4859351.1"
                     /translation="MTESIQSIKERLKSIQRIDDPTLESLRNDPRKGVQQALASFERR
                     LKKEALIKEKYEEMFCFEKQGYAQGHQWIAGIDEVGRGPLAGPVVAAAVILPKNEIIL
                     GLNDSKQLSEKKRELLYQQVQEKALAIGIGIVDQQTIDDVNIYEASKLAMIQAVEKLS
                     IVPSYLLIDAMTLDLPIQQEKIIKGDARSVSIAAASIIAKVYRDQLMKEYDVLYPGYG
                     FSHNAGYGTKEHLEGLERQGITPIHRKSFAPVKKYLQ"
     gene            complement(20699..21559)
                     /gene="ylqF"
                     /locus_tag="IXT20_000885"
     CDS             complement(20699..21559)
                     /gene="ylqF"
                     /locus_tag="IXT20_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HAQ4859352.1"
                     /translation="MTIQWFPGHMAKARREVSEKMKYVDIVFELVDARLPLSSRNPML
                     DQIIQQKPRLVLLNKADLADPQQTKLWQEYFRKQGHLALAITAQESKGIKGLVPAAKE
                     ALKEKRARDAARGIKPRAIRAMVLGIPNVGKSTLMNRLVGKKIAQTGNKPGVTKGQQW
                     LRLGNELELLDTPGILWPKFEDEEIGKKLALTGAIKDQLVHLDDLAIYGLDFFARYYS
                     GRISDRYGLSIEQEEKLAPELLMDITAKRGYKEDYDRGSELVVHDIRQGKLGRYTLDR
                     CEEMEQKSDD"
     gene            21819..22217
                     /locus_tag="IXT20_000886"
     CDS             21819..22217
                     /locus_tag="IXT20_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HAQ4859353.1"
                     /translation="MNSMVFVNFPVADVNRSVAFYEKLGFKKNEEFSTEEAGSMVWDD
                     TFWIMLLSHDFYKKFIKDKEIADNKKTSGALISFSVENADEVKKFAQIAKENGGTFHE
                     VDMGMPEEEMFSLEVQDLDGNTLEPVWMKM"
     gene            22454..22996
                     /locus_tag="IXT20_000887"
     CDS             22454..22996
                     /locus_tag="IXT20_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HAQ4859354.1"
                     /translation="MNTLIAKDVFRLPEMNARLLSTSALLLALDVILYKIAIGPAYFQ
                     ITFGFLSMALMGYLYGPIWAGLLEMLSNTLNFTIFGSGTFQAAFLITAFLGGFINGLF
                     LYHKKINLPSVIAAQLLIMIPVSLFINSWLMSVLFGADFKVIFLGRFIRNMIQIPIQI
                     MVVYSFIKTLEARKVFKRAV"
     gene            complement(23079..23630)
                     /gene="lepB"
                     /locus_tag="IXT20_000888"
     CDS             complement(23079..23630)
                     /gene="lepB"
                     /locus_tag="IXT20_000888"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HAQ4859355.1"
                     /translation="MTKKQRYINRFWLVFKYLLVSVFLAFMLRGFLFIPVPVEGNSME
                     NVLKQGDMVVMEKFSEIRRFDIVVFQLADGTIYIKRVIGLPGENVSYQNDQLKINGKV
                     VKEPYLTKNLKSDHANASYTTDFTLQELTGQSKLPEDNYFVLGDNRRVSKDSRSFGTI
                     NKTDILGKARFVYYPLDEIKWIQ"
     gene            complement(23782..24009)
                     /locus_tag="IXT20_000889"
     CDS             complement(23782..24009)
                     /locus_tag="IXT20_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HAQ4859356.1"
                     /translation="MNRSFFHYLMTLRGGGTHDALSVFATEAGKDIQFPKHSSSYEEI
                     SNYLELNVDYLPSMDIFDEAWEKYLENNHDI"
     gene            complement(24006..24524)
                     /gene="msrA"
                     /locus_tag="IXT20_000890"
     CDS             complement(24006..24524)
                     /gene="msrA"
                     /locus_tag="IXT20_000890"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HAQ4859357.1"
                     /translation="MERAIFAGGCFWCMIQPFDTLPGIHTVMSGYTGGHVPDPTYEQV
                     KSQTTGHTEAVEIIYDPTLISYEQLVELYWQQTDPTDAFGQFEDRGDNYRPVIFYLTE
                     EQRIIAEKSKKQLAESGRFEQPIVTKIEPAQPFYLAEEDHQEYYKKHPENFKRNHARR
                     AAFIEENWRNEG"
     gene            complement(24544..25257)
                     /locus_tag="IXT20_000891"
     CDS             complement(24544..25257)
                     /locus_tag="IXT20_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpmS family protein"
                     /protein_id="HAQ4859358.1"
                     /translation="MKTRSDVSSNKTKKTNYWKFAFLILLGVVLGSTVFLGSRIFANR
                     EPELPEIPALTERQGDPVLTINSNKEKVNQIISFFLSEYQKDSDIEYKFYLENEALLN
                     GTFEVLGFPIDFYLYFDPYVMENGNVQLKARSLSIGTLSLPIKDVMNMIKRNYKLPDW
                     IEVNTDDLTIMLRLDQFRMQNGMYIKADKIDLVNDDIRFSLYLPKDTSDASNNSNTNK
                     TSTSSTESKTKENKKSSDE"
     gene            complement(25260..26156)
                     /locus_tag="IXT20_000892"
     CDS             complement(25260..26156)
                     /locus_tag="IXT20_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HAQ4859359.1"
                     /translation="MKLTKRHLILVLFPLLIAIIIYAGLAASIPKAGPLMKPVTTVRE
                     DPNKKEKLHYIAIGDSLTEGVGDQTKQGGFVPLVANDLKDRYNLTAVETENYGVNGER
                     SDQILKRVKTKEEIRNNIKTADFITLTVGGNDLMKVIQNNLFGLTVNSFKKPLKNYQE
                     NVTELLEEFRSLNKTAPIYILGIYNPFYLNFPEITDMQTIVNNWNKGTETVTKKMPNC
                     YFIPINDLLYQGLNQEVGVTEEGTDQTTSTTENLNGIKNNVLYEEDHFHPNNLGYQLM
                     ANAVRDQLIETQNQWLEKEDDQ"
     gene            complement(26314..27156)
                     /locus_tag="IXT20_000893"
     CDS             complement(26314..27156)
                     /locus_tag="IXT20_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAQ4859360.1"
                     /translation="MTNVKIVTDSSCTMEKNVRDALGIRVMPLSIMIDGTVYPDDDQL
                     DGERFMEMMAKAKNLPKTSQPPIGEFISMYDELGEDGSEVISIHMTKGLSGTVEAARQ
                     ASNLTKTKVTVIDSDTTDQGLSFQVIRAAELAKEGKTVEKILEEVKKVQDNTKLYIGI
                     STLDNLVKGGRISRTTGLLSSVLNIRVVMNFAHSELIPVTKGRGKKTFDKWFNNLKNE
                     LKELPNVRQIGISHADAKELAQEFKEGLQELFPDMHIPVLHTNPVIATHTGKDAFAIM
                     YYVD"
     gene            27288..27941
                     /locus_tag="IXT20_000894"
     CDS             27288..27941
                     /locus_tag="IXT20_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HAQ4859361.1"
                     /translation="MEKTKFNRTYEILNEVFNAVTHGIGTGLSIAGLVVLLVKGAHLH
                     SSLHIVSYAIYGSMMILLFLTSTLFHSLIFTKAKKVFQVFDHSSIFLLIAGSYTPFCL
                     LSIRGWLGWTLFVLIWLFAILGIIYKALTLHKRERVSKVSTIIYIFMGWLCVVAAVPL
                     YHSLGMTGVILMVAGGLSYTIGAFFYSLNSIRYMHVVWHLFVMLGAGFMYFSVLFFT"
     gene            complement(28004..28531)
                     /locus_tag="IXT20_000895"
     CDS             complement(28004..28531)
                     /locus_tag="IXT20_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAQ4859362.1"
                     /translation="MLAAIWAQDENGLIGKEDRLPWRLPNDLKFFKQMTEANTLVMGR
                     KTFEGMGSRPLPNRQTIVLTRDSSYKAEGVHVMHSVEDVLALEKETDGIFFIAGGSAV
                     YQEFLPFCTILYRTVIHHVFDGDAYFPPVDWSDWSLINTSQGEMDEKNRYPHQFETYQ
                     RNENPVKSGTGELTI"
     gene            complement(28551..29498)
                     /locus_tag="IXT20_000896"
     CDS             complement(28551..29498)
                     /locus_tag="IXT20_000896"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HAQ4859363.1"
                     /translation="MEQAYLDLGRKLLEEGHRKEDRTGTGTLSLFGYQMRFDLQKGFP
                     LLTTKRVPFGLIKSELLWFLKGDTNIRYLLQNKNHIWDEWAFERYIKSPDYNGPDMTD
                     FGHRCLTDPEFNEEYKKQSKLFCEKILTDDSFAETYGDLGHIYGYQWRHWETKDGGFI
                     DQIKQVIEAIKKTPDSRRLIVSAWNPEDVPSMALPPCHTMFQFYVQEGRLSCQLYQRS
                     GDVFLGVPFNIASYALLTHLIAHETGLAVGEFVHTLGDAHLYINHIEQMKEQLSREIR
                     AFPTLVLNQEKQSVFDFEMEDIQLEGYDPHPAIKAPIAV"
     gene            complement(29510..31396)
                     /locus_tag="IXT20_000897"
     CDS             complement(29510..31396)
                     /locus_tag="IXT20_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAQ4859364.1"
                     /translation="MLKELKVTDLKKTYGEKDLFDQISFLVHEKDRIGLIGTNGTGKT
                     SLLNIIAGIDSSDGDRQTVFYPTDYRIGYLSQAAEFSDELTVLQAVFQGNSPLIQTVR
                     AYEEALIELGENGEDPDVQKRYAKAEEQMNKEDAWTTDTNAKIILQKLGISELDKKIS
                     TLSGGQKKRVSLAQVLIDEPDLLLLDEPTNHLDYEAIEWLENYLKQYRGALLMVTHDR
                     YFLDRVANRIFELSFGKLYEYKGNYEAYVLEKAERDRVEVEQEEKRKRLYKQELAWMR
                     AGVQARGTKQQARINRFEDLKENLYQVNQEDDLELNLATQRLGKKVLEIKDGSYRIND
                     QTLLEHLDLLIQSRERLGITGKNGAGKSTLLNILAGRIPLDSGTMSIGETVHLAYYTQ
                     ENEEMAPDKRMIAYLQEAAEEAKTADGSQIGVAELLERFLFPRFMHGTLIGKLSGGEK
                     RRLFLLKLLIQQPNVLLLDEPTNDLDIATLTILEDYFRSFPGAVITVSHDRYFLDKVA
                     DKLLVFQGNGKQELYYGNMSSYLLKQKETQQPAEKAKPKIQSKEPAEKKKLSYMEQKE
                     WETIEDEIAELEEKISLLQEEMNHQGDNFTRLQELQNDVSETEAQLEEKMARWEYLSE
                     WVED"
     gene            complement(31556..33634)
                     /locus_tag="IXT20_000898"
     CDS             complement(31556..33634)
                     /locus_tag="IXT20_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HAQ4859365.1"
                     /translation="MEFAELVIVFAVTITLSNIASRILPMIPAPLIQIFLGVVLGLTE
                     WGQSIDFEPELFLVMIIAPLLFREGEKADISSILKNFGTILFLAFGGVILTLVGVGAT
                     LSFLLPSVPLAACFAFGAALGPTDAVAVSSLSGRVNIPKKAMHILEGEGLLNDASGVT
                     AFQFALGALITGSFSAVNAGMSLVVSSIGGALIGFLLVWFKQKIIHLIEKASAQDVTG
                     YLLIELLLPFLAYVLAEAFDVSGIIAAVAAGILQASGFRKISVFDAELSSLSHSTWTT
                     IAFTLNALVFIFLGIELTQVFSPVWGDGLYPNGLLLAIIVLISVMLFVIRFISISLFY
                     VFKDGSKKFKKQLNEILILTFGGVKGTVSLATIFILPFSINNMMFYQRSLLLFLTAGV
                     ILVTLVIGIIVLPMLTETEEAKSTDLNALMILEEVVEVLRKEIKEIDRNTKEFLATEA
                     VIENYQERIRDLYLEDLTDDEKQEVQEIQALILSIERDGLDESYRSGKLSSNGYRFYS
                     RFLSRFEHSITSQILSFIGFWFIVVRRLMRIVLHPKMFWQRRYADRQTFISDEDIQEI
                     RETYQKNTQLIIESLDNLTDIYDETMVQFFIQQRKLEGIKMNSGNLISSWMIQQDSLF
                     TKKMLRGYYLERKIIDEYEVAEKITTFSANSYRRNINLLESYTMNKPSDNFSFRFAFR
                     AKSKKTSPIK"
     gene            complement(33647..33922)
                     /locus_tag="IXT20_000899"
     CDS             complement(33647..33922)
                     /locus_tag="IXT20_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4859366.1"
                     /translation="MRESFEQQKKLLHDRYGALSMDDRRQILCKLRKRNILMYRQLER
                     LKHDLLRLESKRVQCELEGNQTQVEVVETKILKKKEQFLKMLTQNKK"
     gene            complement(34284..34739)
                     /locus_tag="IXT20_000900"
     CDS             complement(34284..34739)
                     /locus_tag="IXT20_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4859367.1"
                     /translation="MSKEMTALKFYFRNGETWTINRRHIGDLWIKQITTSFGRINGSE
                     FVEIHPCAGFKIEIFHEGDAVATHDINLGGLEMGMFNRALKYEDIERMEILYRNGTPD
                     LVYFPYLDKGTEGLDNQYQSTKISEKTGNLYIVINPDQRVEDVYGEFFE"
     gene            complement(34893..36101)
                     /locus_tag="IXT20_000901"
     CDS             complement(34893..36101)
                     /locus_tag="IXT20_000901"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HAQ4859368.1"
                     /translation="MKLKQLPLEYQKAIPVLKKLENAGYEAYFVGGSVRDILLNQPIH
                     DVDIATSAFPEEIKQLFPKTIDIGIEHGTVLVLENDEQYEITTFRTESTYQDYRRPDH
                     VEFVRSLAEDLKRRDFTINAFALKEDGEIIDLFDGLQDLEEQILRAVGNPHERFHEDA
                     LRMMRGLRFVSQLGFKLEEETFYAIKENHELLGKISVERINVEFIKMLLGNERAAGIH
                     AFIETECYICCPGLKEEGEALLRFAELPSVPLKEESQAWALLISQLDLKENEIRSFLK
                     SWKCSNQMIKDVQALVQGLRQRETGALEPFELYFLGKKHALQVEELMIYFDETPQMDA
                     VGESYDRLPIKSLSELAVDGKILLEETGKKPGKWVSEALQLAEKAVVEQRIKNDKKQV
                     LDFLSKEKLI"
     gene            complement(36213..36545)
                     /locus_tag="IXT20_000902"
     CDS             complement(36213..36545)
                     /locus_tag="IXT20_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HAQ4859369.1"
                     /translation="MDKKRSLYSMQQEVDEYIQQFKVGYFSPLAQMARLTEEVGELAR
                     EVNHTYGEKSKKASEPVNSVAEELGDVLFVTMIMANSLNIDLTEVFEKNMEKFNQRDH
                     NRFERKDE"
     gene            36693..37568
                     /locus_tag="IXT20_000903"
     CDS             36693..37568
                     /locus_tag="IXT20_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAQ4859370.1"
                     /translation="MVKRKTVKEVLFIIIGTSIYAFGLVYLNIANHLAEGGVSGITLI
                     LRALFGIDPAYTTLLINIPLILLGGKILGKRSLAYTVLGTLSLSFFLWIWQRVPLEIN
                     LQHDLLIVSLLAGLVAGVGSGIVYRMGGTTGGSDIIARILEQNYGITMGRSLLAFDII
                     VLLASLTYIDLKRMMYTLIVSYVFSRVIDSILDGGYSAKGLLVVSNKSEEIAPLLMTG
                     LQRGVTFLSGEGAFSGNSKKIIYMVVSSSELTEAKRIVHEIDEKAFLSIINVHEVEGQ
                     GFTYLKPQTRLLKKL"
     gene            complement(37650..38231)
                     /locus_tag="IXT20_000904"
     CDS             complement(37650..38231)
                     /locus_tag="IXT20_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IDEAL domain-containing protein"
                     /protein_id="HAQ4859371.1"
                     /translation="MLIDVREKKNFLTWMVNHVSFSRREVFWILNYLANHEAILSNVH
                     FVEGANFADRGLQISDLSVEGEPMKLFLQGKEFTDTDQIFHEIRLNWKKPLYVECIFE
                     NAWQTREYLSILEDNPFSSWDSSVSEEDTELIERYFQEKEEEAKLKMLYAQIDQALED
                     GDRDAFLKLSDEVNRKILLQTQKKSNRVSEHNN"
     gene            complement(38237..39496)
                     /locus_tag="IXT20_000905"
     CDS             complement(38237..39496)
                     /locus_tag="IXT20_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HAQ4859372.1"
                     /translation="MNTNSEKMLQALSDEDLSQAQIFLEKALKEDPADILAELGEELL
                     AIGFLIEAKQIFEQLINQYPENDGLNIPLAEIAIEDNEIDLAFEYLEKVDKQSDYYPQ
                     SLLAIADLYQVIGIPEVSEAKLKEAAGLLPDEPLIQFALAELYFSVDRFKEAAVVYET
                     LLASGINEISGISMQERLGQSLSMQGEFEAAIVPLEEALKEERTDDRLFQLAFTNLQL
                     KENQQAINYLEELREVNPHYQSLYLYLGQALQEEEMIEEAQTVLEEGIKENPYQVELY
                     HLASENAFRLHDRQRAEELLLKALEVGDKQDETLLTLSNLYLDDERYEDVIQVINQME
                     ETANPYAEWNLAHAYNELEDFTVAAVHYEQAYHELSHEPDFLKEYAFFLREEGQLKRA
                     QELLTYYLTLEPGDMEALSLLDDLTER"
     gene            complement(39498..40544)
                     /locus_tag="IXT20_000906"
     CDS             complement(39498..40544)
                     /locus_tag="IXT20_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4859373.1"
                     /translation="MELDYQVEDALTKVFYGETLVSQLRELSIDQPILFLTNQRYYDL
                     FADKINPLFANQANNDWYICANTQCNHLTELKNLLDFVKRYPENQSMFIIGFGNEGIM
                     DLAGFFQKHTTLDSSLWMIPVSIRSLSRALTPQRTILQLPNRVVLQTINLPERIVYDQ
                     TISERQVDGKQIDFLTFICCGMICDHRFLQNLYKNYPNKKQLTTRPFAGMIEEMIYFY
                     QLEAENLSNYGKLFEQAFYLTENGHLLSASMKKFLGMMFHFVWNLQAYPFDFQLKNFF
                     VWLDLLGYPIHWLEQISKMEYLEKVLFLAEKSKKILVLEKIGIIDGYQHPNEKELLRT
                     MTVFQQIVSEIRGN"
     gene            complement(40779..41054)
                     /locus_tag="IXT20_000907"
     CDS             complement(40779..41054)
                     /locus_tag="IXT20_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008214712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HAQ4859374.1"
                     /translation="MANKAELIEKVASATDLTKKDATAAVDAVFSTIQDALANGEKVQ
                     LIGFGNFEVRERAARKGRNPQTGEEIEIPASKVPAFKPGKALKDAVK"
     gene            complement(41297..42607)
                     /locus_tag="IXT20_000908"
     CDS             complement(41297..42607)
                     /locus_tag="IXT20_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HAQ4859375.1"
                     /translation="MANPTIAIVGRPNVGKSTIFNRIAGERISIVEDTPGVTRDRIYA
                     KGEWLGREFSVIDTGGIDLGDEPFMDQIKHQAEIAIEEADVIICVVSGREGVTDADEM
                     VAKILYRSNKPVILAVNKVDNPEMRNDIYEFYSLGLGDPYPVSGSHGLGIGDILDEAV
                     KYFSEETEEEDDDTIKFSLIGRPNVGKSSLINAILGEERVIVSDIEGTTRDAIDTHFV
                     SESGQKFLMIDTAGMRKRGKVYENTEKYSVMRAMRAIDRSDIVLMVLNAEEGIREQDK
                     RVAGYAHEAGRGIIIVVNKWDLVKKETNTMRDFEQEIRDEFRYLDYAPIVFVSAVTKQ
                     RLERLPEMIEQVSMNQNLRISSAVLNDIIMDAVAINPTPTDKGKRLKIFYATQVAVKP
                     PTFVVFVNEEELMHFSYERFLENQIRKAFTFEGTSIRIIPRRRK"
     gene            complement(42789..44018)
                     /gene="rpsA"
                     /locus_tag="IXT20_000909"
     CDS             complement(42789..44018)
                     /gene="rpsA"
                     /locus_tag="IXT20_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HAQ4859376.1"
                     /translation="MTEFDQNQENNQSMEDAMKSVQEVKVGDVVKGEVLVIEDKQVIV
                     GIEGAGVEGVVPAKELSTLPVEDISEVVKVGDVLDLVVISTIGKDKENGSYLLSKRRL
                     DAKKVWEDIERDFQEGKVIEAPVTNVVKGGLVVDVGVRGFVPASMVEDHFVADFSDYK
                     GKTLSFKIIEIEPSENRLILSHKAVVENEREEKKKEILSTIHEGDVLSGRVARLTDFG
                     AFVDLGGIDGLVHVSEISHSHVAKPSDVLAVNDDVNVKVLSINPEQERVSLSIKDTQP
                     GPWSEIDTKAPVGTVLDGTVKRLTSFGAFVEVFPGVEGLVHISQISHKHIATPHEVLH
                     EGDEVKVKVLEIHPEEHRIALSIKALEEKPASEEEPKNVESYELPEENTGFTMGDILG
                     DALKEEVEEKATDDSEK"
     gene            complement(44153..44833)
                     /locus_tag="IXT20_000910"
     CDS             complement(44153..44833)
                     /locus_tag="IXT20_000910"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HAQ4859377.1"
                     /translation="MTQISIAIDGPASSGKSTVAKILAKELNYIYTDTGAMYRAVTYL
                     AITNNVSFSDEEGLVSLIKKYPISFSQQEDGQHVFIASKDVTLEIRQPDVTKAVSEVS
                     AHGKVREELVAVQRKIGERGGVVMDGRDIGTAVLPNAEVKIFLVASVSERAERRFKEN
                     QEKGIPTDLETLTKEIQERDHYDSTRDVSPLKQAEDAVRIDTTGKSIPEVVAAIKAVV
                     LKKGYLLF"
     gene            complement(44882..45517)
                     /locus_tag="IXT20_000911"
     CDS             complement(44882..45517)
                     /locus_tag="IXT20_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAQ4859378.1"
                     /translation="MSRKDRHQSTENNETQEPWEQPIYDTDDEVSSRSSQRQQKKGNT
                     LFLSLFLILLVLCIAIPAGAYFWIRNGSSNNATAATSSETSTSVVESSTSTTKEESTE
                     TSTVESSTIAEESTVSQDTGVTSESSIAAETTPTSSSEATYQAGDSSSNAAETTPSSS
                     AAAGSTTTVQAGEGPKQVAERAGITTDQLFELNGLDPNNFMLYPGQELRIK"
     gene            complement(45583..46986)
                     /locus_tag="IXT20_000912"
     CDS             complement(45583..46986)
                     /locus_tag="IXT20_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HAQ4859379.1"
                     /translation="MLINELKKYFGFDSFRPGQEEIIQALLDGEDTLAILPTGTGKSL
                     CYQLTGYLMEGLVIIVSPLLSLMEDQVTQLQKRGEKRVAAFNSLLSQSERRYVLRHLS
                     QYKFLFLSPEMLTNPSLLEHLKKQNIALYVVDEAHCVSQWGVDFRPEYQQLGKIRKLL
                     GNPVTLALTATATDLVARDIRHVLFDRPPKEIRQSVNRQNISLFVRKTQQKEQELEHF
                     MERAQGAAIIYCATKKEVERLYHLFRERFTVGYYHGGLDAAQRRQLQQQFVKKQLQFL
                     IATNAFGMGIDKSDIRYVVHYDLPDSLENYVQEIGRAGRDQEESAAILFYQSGDERIH
                     YFFNQLSREQRQSFELYLEYATEQAPFDELQKKWMELIQQSEKPENWVERLKRQEKEK
                     EFRLQQMLRYINEENCRRKFILAYFGEKLSEKPQNCCDIDGAKAELLPKRKLFSKTEQ
                     LHWETILLNLFKKNMEH"
     gene            complement(46973..48004)
                     /locus_tag="IXT20_000913"
     CDS             complement(46973..48004)
                     /locus_tag="IXT20_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4859380.1"
                     /translation="MIVFILSLFSSGHKLRISSLYQLLVGKRTTSVLIYGFTHELLFI
                     HNSFPELKQDKFYQILQKIAQQGWIEINENEAKLTPAGADMLSEHQIEHTGLRFDRYG
                     RTGETSWRLIKFAVQVISNLATGIQDYLPAETSPFYTFQLKKWLSESQLPREILIDTA
                     YESLVQLFSEIPEEAADFLANQLSGNERTGLLPYQLAKNNDESAVYLQQSRCIHLLLA
                     QIEKRPDSLWHALIDSLLQQNFNQSMMITKQMFMNGQTIDQIMAIRHLKRGTVTDHLI
                     EWALFFDDFPYEWILSAETIERLEPNKDSVREWRFSEWNVDGQLDYGEFRLYQIYLLR
                     KEAIQNVNK"
     gene            48035..48256
                     /locus_tag="IXT20_000914"
     CDS             48035..48256
                     /locus_tag="IXT20_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HAQ4859381.1"
                     /translation="MSLLCKLVPERCIACGLCQIEAPDIFDYDEEGIVLFAQEPEAHQ
                     QFVMPEEETYVKKAYQKCPVRAILLENKI"
     gene            complement(48421..49158)
                     /locus_tag="IXT20_000915"
     CDS             complement(48421..49158)
                     /locus_tag="IXT20_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase"
                     /protein_id="HAQ4859382.1"
                     /translation="MKIFRRILIGLVIILGLLGGAGYYYIQQNTYEPSSEAQVLVKEG
                     KNKEDYLFFEGEKKNHGKSPLLIFYPGALVEPESYSIWAQDLAKNGFSVAIVKMPLDL
                     AVMGGNKADKVKEDFPNSDYVIGGHSLGGVMASRYVNKNHSDKHLKGVFFMASYPDKK
                     GTLTESAVPVLSITATKDKVLNQEAYQEAKQYLPERTEYVSINGGNHGGFGSYGEQKG
                     DGEAEISNADQQTVIVNTLENWLKKLK"
BASE COUNT        13321 a        10444 c         8470 g        17077 t
ORIGIN      
        1 ttcttcggcc tttagtatgt tctatgtttc tttttatatc aatcgcttat ccattcattt
       61 tgaatgcttc atactaccat ttgattagct aaatacagcc gtcaattgag gtacgacttg
      121 ttttttacgt gaaacgacac ctttaagcaa agcacggttg ttggtcaaag ttgtttggaa
      181 agcttcttct actttttctt ttcctttacc agcaacaagt aattctgaat cactgtctaa
      241 gatgtttgtg acaactaata cgaacagatc gtagccattt gctttcattt catcttccat
      301 tgctgcttct agctctgctt gtcgatccat cacttcatta aggtccactg tattgacttg
      361 agcgatacga acgttggaac tgcccattgg gaaacttttt gcatccaaat caagaagaac
      421 cgcagctgat ttatcactta gatttgttcc tgctttcaac atttcaagtc cataagtgtt
      481 tagatcgact tcagcgattg ctgcaagttc ttttgcagct gcttcatctt caggcgtaca
      541 tgttggtgat ttgaacaaca atgtatctga aatgatagct gagagcatga ttcctgcgat
      601 ttctttagga attgttactt ggttttcttt atatagtttt aaaataatcg tacttgtaca
      661 tccaactggt tctgcacgat agtacaatgg attagctgtt tcaaagtttg caatacgatg
      721 gtggtctact acagctaaaa tatttacttc gctgatatct gaaatacttt gttggaattc
      781 attatgatcc acaagcatga catctgcatt ttctgcttta gctgactcta ctactcgtgg
      841 tgcagatagc tgaaagtggt ctaatgcgta ttgtgtttct tcattaggtg tacctagagc
      901 aactgcttct gtttctttac ctaattcatt ttgcaagtgt gaaaacgcaa tggctgcccc
      961 gattgcatct gtatctggat tttggtgacc aaaaactaaa atttttgaca tgtcattcgc
     1021 tcctttgtga tttctcctat ttaataatag caaaaaaacg gaaagaagca atacttatct
     1081 tctttccgat gcgacctatc gcgttaaata ttttgtatac tcttctgtat gaagcaatcg
     1141 tttagcattt tcgacacgtt cttttgtagg tggttcgatc ccttctaatg gatatttcaa
     1201 accaagtgtt tcccacttgt attttcccat cgtatgataa ggaagaatct ctactctgtc
     1261 tacatttgac aaggtttgaa taaagttgtc tagtcggatc aaatattcat cataatcact
     1321 tcgctcagga actagtacat ggcgaatcca aaccggtttg ttgatttttg acaaatattg
     1381 agccatttct aaaatatttt cattgtccca atgcgttagt tttttatgct cttcattatc
     1441 gatgtgtttg atgtcaaata acagtaaatc tgtatacttc atcaattctt gaaaacgtga
     1501 aaagaaaggt tcttcatacg taaaaggttt tccgcatgta tccaatgtag tatgcatctt
     1561 ccgctctttt gcttttttaa atagttcgat caaaaaatcg atctgcaata gaggttctcc
     1621 accactaaca gtaataccac ctttatctcc ccagtaaggt ctgaagcgtt ctgctttatc
     1681 taaaagttca tctgctgtgt attctttccc tccgccaata ttccaagtat ctggattgtg
     1741 acaaaattgg caacgcatgc ggcatccttg cataaacacg acaaagcgca gtccaggtcc
     1801 gtctactgaa ccgaaagttt cgatagaatg aacatagccg attgtttttt cttccattta
     1861 agttcacatc ttttctttga tttcatttta agtacaagtt agctgtcgaa gcgaagcttt
     1921 ctgaacaaaa tttttcgcaa ttgccgcttc gttatgtgta aaatttacag ggacggaaca
     1981 tacaagtcct gcccctgtaa atcgtttctt tcaaataagc atactgatta ttcgattttg
     2041 atgcgatggc tattcaacgt cctttcgacg ctaagtcgaa aaactttttt cacgaacttt
     2101 ttacattctg tcgtgtgaag ttctcatcaa gacatctaat tgttgttctc ttgtcaagtc
     2161 acggaattta actgcataac cagatacccg gatcgtcaag ttcggatatt tcattggatt
     2221 ttccattgca tcacgcaata aatcattact gaatacattg acattcaaat gataagctcc
     2281 ttttgagaag tatccatcta agacattacg caagttttcg attcttgtat cttgttcttt
     2341 tccaagacct gaaggattga ttgtttgggt atttgaaatt ccatccaatg cacagccgta
     2401 attcatttta gctgttgagt tcaggctagc taataaccca ctttgttccg cattgtaact
     2461 tggatttgct cctggcgcaa gtggttttcc agcttcacgg ccatcaggag tatttcctgt
     2521 atttttaccg taaacaacat ttgaagtaat cgtcaataat gaagttgttg gttttgagtt
     2581 gcggtaagtt ttatgacgtt tgatttgtcc catgaagtat tccaagatcc atgaaactaa
     2641 ttcatctgca cggtcatcat catttccgta tttagggaag tcgccatcca cttcaaaatc
     2701 aactgctatt cctttttcat tacggattgc ttttactttt gcatatttga tcgctgacaa
     2761 gctgtctgcc gcatgagaga tcccagcaat acctgtagca aatgtgcgtt gcaagtcaga
     2821 atccatcaaa gccagctgag ctgcttcata agaatattta tcatgcatat aatgaatgat
     2881 gttcaacgta ttgacatata gttctgctaa ccaatccatc atattcatga agttatgttt
     2941 cacttcttca aagtctagta tttctgatgt gattggacga tattctggtc caacttgggc
     3001 gccagtcact tcatcgactc cgccattgat ggcatataat agtgttttag ccaagttagc
     3061 acgagcgcca aagaattgca tgtcttttcc gacaactgtt cctgatacac agcaagcaat
     3121 cgcacaatca tcagaacccc attgttcacg aagctgatta tcattttcga attgaatcga
     3181 agaactttcg atagcgattc tgctagcata tgttctgaat ccttctggaa ggtgttcaga
     3241 atacaaaaca gtcaagtttg gttctggtga aggtcccata tttgttaatg tatgaagcag
     3301 acggaaatca ttctttgtaa ctaatgaacg accatctaat cccatacctg caatggataa
     3361 agtagcccag atagggaatc ctgagaataa ttggttatat tcaggtgtac gcgcgaattt
     3421 gaccatacgt aatttcatta ctaagtggtc gatcaattct tgtgcttctt tttctgtgat
     3481 tactccatta tccaaatcac gttggatata gatatcaagg aatgctgaga tacgtccgat
     3541 tgacatcgca gcaccatttt gtgatttgat tgctgctaaa tatccgaagt atagccattg
     3601 gacagcttct tttgcatttg cagctggttt agaaatatca tagccatatt tcgcagccat
     3661 ttctttcatt tgcgctaatg cacgatattg ttcagtgatt tcttcacgta aacgaatcac
     3721 ttcttcactc attgtatgat aaccagtcat atcatgatct tttttctttt gttccatcaa
     3781 atagtcgatt ccatacaatg ccacacgacg atagtcaccg atgatacgtc cgcgaccgta
     3841 agcgtctgga agccctgtaa tgattttgtt tttacgagca gcacgcattt cagcagtata
     3901 agcgtcaaat acaccttgat tatgtgtttt gcgccagtca gtgaaaatat gtgtcaattc
     3961 atcgtttggt gtatagccat tgctttttag cgcattgtta gccatattga ttccgccgaa
     4021 cggcatgaat gcttgtttca aaggtttgtc tgtctgtaga ccgacgattt tttccaaatc
     4081 tttatccaaa tagcctgcat cgtgtgaagt aatagttgat acgatttctg tgtccatatc
     4141 atagacaccg ttacgctcga attggatatc atttaacttt tgcaattcat cccataattt
     4201 ttctgttgct tctgttgggc cttccaaaaa gctgtcatca ccgcgatatt cagtgtagtt
     4261 attttgaata aagtctctag tgtctacgcc ttctttccat ttggtacctt taaatccatt
     4321 ccattgctcc attgtatgga cctcctaaat ttctgtttta tgtaacagtt tttacgtgat
     4381 tagtataaca cagcacccta tctttgcaag ggttttcttg ttttttattt aacaattttt
     4441 atgttttatt tacatatatt taaatcaacg ttgacatacc aacatttaag cattgtgaac
     4501 actttcatct ttttttgcaa acgtataagg atttattaaa aatgaatgaa aaagagacgc
     4561 cacctgtatt aatacagatg gcgtctcttc attaaaatac tacaacagtt acgactcgat
     4621 gaaaatctca ggagcatcga taacttcgat gacttctcca ccttttttct catccatcgc
     4681 aaatgatcca ttcgatgttc gatcacctat agggaaatca gttgggtcaa tcgttaactg
     4741 ctgccctttt tgattcatca aggttatttc ttttgatctt ccttccgaca tatagataat
     4801 acggtgcgga ttttttttca gctcacgcat gaccgtcact ccccgcttcg cacgtgaagt
     4861 ttggctgatt tcttgagcaa gcatgcgttt aacagctcct cgctgagtaa cgattacgat
     4921 cggcgtatca ccttctgcta acacaagcag cccgttgata atcctatctc catctttcaa
     4981 attcatagac ttcactccag ctgcctttgc tccaacaacg ggaacatctg ttaaaggata
     5041 gcgcaaaccc attccttgat gcgttactaa gaaaacatct aagcctggct gatcttcttt
     5101 cagatacaca gcaatcagtt catcttcttc cgcctttagc ttcatacagc tgagcggacg
     5161 gctcttatag gtgcgccatg gttcgaactc tgtcattcgt gtttgcttga tcaatccatt
     5221 cttgctgatg aatacaaatg ttttctctgc ttgaattttt tgatatggga aaacagctaa
     5281 aatcgattcg tctacagaca gattggagat tgtttgagaa atgtgctcac cagcatcttt
     5341 ccatttcaaa tcgggtagtt catggacagg gcggtagatc acatttgctt tatttgtgat
     5401 cagaagaaca tggtccaatg tactcaattc acctgagtat agcaagtagt cgccttctcg
     5461 catcccgatt tcttctggtt tggaagcact ataagaacga aggcttgttc gcttcacata
     5521 gccttctcgc gtcacactga caattacgtc ttcttgtgcc actaagactt gcgtatcaat
     5581 tttgatttct tcgatttcgt cttcgattgt cgttagacga gcggaagaat aatttttttt
     5641 cacttctcgc aattcttttt tcataacaga gaaaagctct ttgtcatttg aaaggatttt
     5701 attcaactct gtgatttgcg caatcaagct ttcggattcc ttttgaagct ctgtgatatc
     5761 cgtgttcgtc aaacggtata attgcaatgt gacaattgct tctgcttgtt cttctgtaaa
     5821 ttgaaagacg tcgactaaat tctttttggc gtcttttttg tctttacttt cacgaatcgt
     5881 tgcgattact tcatccagaa tcgataaagc tttcatcaat ccttctacga tatgctgtct
     5941 tttttgagct ttggctaaat cgaattgact acgttttgtt atgacttgct ttcgatgctc
     6001 gatataactt cttaaaatgt ctttcagtcc aacttgatgc ggagtcatat ggtcgatagc
     6061 gaccatattg aaattgtaat tgatctgtag ctcagtgttt ttgaacaaat aattcaaaat
     6121 tccttgagca ttggcgtctt ttttcaattc gacgacaatt tgcagacctg ttctatcact
     6181 ttcatctcgt acttcagcta ttccatcgat tttcttattc aaacggatct catccatttt
     6241 tttcaccaag gtcgctttat ttacttcata tgggatttct gtgatcacga tttgctgttt
     6301 attacccttc attgcctcta tctttgtttt cgaacgaaga ataacttttc ctcgtcctgt
     6361 ttcataagct tttttgattt catcttttcc ttgcaagatc cctcctgttg gaaagtcagg
     6421 tccaggaata tattccatca atttttcaag gctcgcttgt ggatgatcga tcatatagat
     6481 tgtgccatcg atcacttcag ctaagttatg agtcgggatt tctgttgcat atccagcaga
     6541 tatacctgtc gatccattga cgagaaggtt tgggtatcga gctggaagga ctgttggttc
     6601 tttttctgta tcatcaaaat tccacacgag atcgactgtt tctttctcga tatctgctag
     6661 catctctcca cttaacttgg ataatctggc ttcagtataa cgcatcgcag caggtggatc
     6721 accgtccata ctcccgttat tcccgtgcat ttcgatcagt acttcccgca acttccagtc
     6781 ttggctcatg cggaccatcg cttcataaat actgatgtca ccatgaggat gataattccc
     6841 catgatattt ccgacagatt ttgctgattt acggaatcct ttgtcatacg tattgccatc
     6901 tttgttcatg gcaaataata tccggcgttg cactggcttc aatccatcac ggatatcagg
     6961 cagtgcacgt tcttggataa tatatttgga ataacgtccg aagcggtcac ccatcacttc
     7021 ttctaaagtt aattcttgta cttcttgtcg attttccatc aggtgacctc ctattctact
     7081 tcaaataact gattgttttc atttttatct gctcgttctt catctaataa atcattagaa
     7141 actgaaggag agatttcagt atcctctttt ttatctaaga tactgccatc ttcttcgagc
     7201 gtaaattgca cgtgattttc gatccatttt cttcgaggtt ctactttgtc acccatcagt
     7261 gtggttactc gacgttcagc ttgcgcagca tcatcgatac ggacgcggat caacgtacgg
     7321 gaagtcggat ccatcgttgt ttcccacaat tgttccgcat tcatctctcc aagtccttta
     7381 tatcgctgca gcatatatcc tttgccgact tttttaatca ttgctgccaa ttcgtcatct
     7441 gtccacgcat attcaatgac ttgcttcttt ccttgtcctt tggatacttt atataaaggc
     7501 ggtaacgcga tatatacttt cccagcttca atcagcggtt tcatataacg ataaaagaaa
     7561 gtaagcagta atacttgaat atgggcgccg tctgtatccg catcggtcat gatgattact
     7621 ttgtcgtaat tacagtcttc gatagaaaac tcaggaccga ctcctgcacc aatcgtatag
     7681 atcatcgtat tgatttcttc atttttcaag atatcttgca ttttagcttt ttcggtattg
     7741 atgacttttc ctcgtaaagg tagaattgct tggaactttc tgtctcttcc ttgtttagca
     7801 gatccgccag cagaatcccc ttcgactaaa tacaattcat ttttcttagg gtttcgggat
     7861 tgtgcgggtg tcaattttcc tgaaagaaga gattcgcctt ttttacgctt cttcccattt
     7921 cggctttctt ctctggcttt acgtgctgct tctctcgctt ctctggcttt gattgcttta
     7981 cggaccaaca tctggctcat ttcgctattt tcctgcaaat aaaagcccat ttgttcactg
     8041 atgacgttat ctactacggt acgagcaact ggcgtaccaa gtttttcttt tgtttgccct
     8101 tcaaattgca gaagattttc aggtacacgg atagaaagta cagcagctaa cccttctctg
     8161 aaatcactac cttccagatt cttgtcccgt tctttcagca aaccaacttt tctcgcatat
     8221 tcattataag atttcgtcat cgccgctttc ataccagctt catgggttcc cccatctttt
     8281 gtccgaacgt tattgacaaa agataaaaca ttttctgaat aaccgtcatt gtattgataa
     8341 gcgacctcta cttcgatacc ttctttttct cctgaaaaat aaacaactgg tgtcaatgta
     8401 tctttttctt cattcagata atcaacaaat tctttgattc cttcttcgta atgaaaaatt
     8461 tccttgactg gttcttctcc acgaagatca gaaagagaga ttttaacgcc ttttagaaga
     8521 aaggcagatt ctcttaaccg ttcggctaaa atttcatatg aaaatttcgt tgtactgaat
     8581 attgtatcat ctggtaaaaa atggacagtc gtcccgtttg ctttttttgt ttttcctatc
     8641 tttttcaaag taccgtcagg tttaccgccg tttttaaatt tctgctgata ctctacgcca
     8701 tctctaacga tcgttacaat tagccatttc gataaggcat ttaccacact ggctcctaca
     8761 ccgtgcagtc caccagaagt tttgtaccct ccttgaccaa actttccgcc agcatgcagc
     8821 acagtaaaaa tcacttcaac tgttggtatt ccggaagcat gcattcctac aggcatccca
     8881 cgtccgctat ctgcaacagt tatactattg tcttcatgga tggtcacatc aatctcactg
     8941 ccataacctg ataatgcttc gtcaactgca ttatcgacga tttcatagac taaatgatgc
     9001 aagcctcggc tgtcagtgga tccaatgtac atccctggtc tttttctaac tgcctctaat
     9061 ccttccaata cttggatgga cgagtcattg tattcgttgt ttactttttt tgccaaggca
     9121 aacgctcctt caataatact ttgcggcaaa accgcaccac gattatcata ctagaaaagg
     9181 tcgtgaaaaa aaagagggta gacgagaaat caattttaat tatctcctaa ccgctttttc
     9241 gacattcccc tctatcgatc atctcttctt tgtggtcatt tttatttttg gttttctttc
     9301 aacttaccaa gttcaatctt aatacaacga tccattacga tatctgtacg tcctgctttt
     9361 cttagcatct cagctgcttc tttgttttca atccctaatt gtgcccagaa cactttagca
     9421 tcagttttca aaaaatcacc tgctatttca ggtaaaaatt cactccgtct aaatacatct
     9481 acgatatcga ttggatcatc gatttctgtc aaattcgcat agactttctc gcctagaatt
     9541 tcttcaccag ccaataatgg atttactggt atgattttat atccattgtc ttgcaatacc
     9601 tgtgcaacca tgtaacttgt tcgttctggt ttattgctca agcctacgac ggcaatattt
     9661 tttgcttctt ccaaatagtt gaaaatcgtt tcttgtgttg gattttttac agacatttct
     9721 gccacccctt gcttttcttt ttattccatc tattatactt gtatctttca ttttttcaat
     9781 tgttcctgtt acctattcat gctaaaataa aggatagttt tcaacttgaa agagggtaaa
     9841 aaatgaaatt gatcttatta cttattttag catatctttt aggttctatt ccttccggtg
     9901 tctggattgg caaattgttt ttcaaaaaag atatccgaca acacgggagc ggtaatactg
     9961 gaacgaccaa tacatttcgt gtattaggca aaacagccgg gattgttgtc ctgcttatgg
    10021 atattttaaa aggtacattg gctacttgtt tgccgatgat cttccacctt tctgggatta
    10081 accctctttg gtttggggta tgcgccattc ttggccatac atttccgatt tttgctgggt
    10141 tcaaaggagg aaaagctgtt gcgacaagtg caggaatgct tttaggattt aatcctgtat
    10201 tttttgttta ttcttgtatc atctttatcg ttagtttatt ttgtacaagt atggtgagtt
    10261 tgagcagtat gatcagtgca gtattgatca ctttatctac gatcgtcctt ccttatgtag
    10321 ctcctgtgat tttggctaag cccaattggt tactgacgat cattgccttt ttagtttctt
    10381 cctttatctt ttatcggcat aaagacaata tcaagcgtat cagaaaccat actgaaagtc
    10441 gaatcccgtt tggtttgaat agcagcaaga aaaaatcctg atactttttc attccaaaga
    10501 gataaaacgg gagtgtgaca aatcgatgtc acactccctt attacttaca ggatagcttt
    10561 tttacacacc ttggtaaacg gtgttccctg ctaacttctc ggtcacaacc tcttacttca
    10621 ctgtgatgga atattttgtc ttgaagattt catgggcacc tagttgattg atccctactt
    10681 tttccatcaa gtttccacta ctgttcaccg tgtcagcgat tccgcaccac ggttcgatac
    10741 aaacgaaagg cgcttctttt ggataaggag accagatccc tacataaggc atatctttat
    10801 agcttaaagt tacgctatgg gaagattttt cacttcgaat ggcgtacgca tttagacccc
    10861 ttgtttcaaa aaccaaggca tcttgctcaa aaagttcacg attcaaagcc agattcgtat
    10921 ttgtctgacc taacgtcttt tgatccatat cgatgaatgg gcctttcaat ggtaggcgta
    10981 atcttgattt caatggagaa aatgccagat agaaatcttc aaatgacaat ccttcttcaa
    11041 gaggtacatt gaaagcagga tgtccaccga tagaaaagaa catttctttt tcaccggtat
    11101 ttgccacttg ataatgaacc gtgatgccgt ctcctcctaa ttcatagctg atcactaatt
    11161 caaaatcaaa tggatacttt tctaatgtct ccggcgtact ccttaagatg aaactagcca
    11221 tttcttgtcc atgttcaatg acttcaaaat ccatatcccg agcaaatccg tgttgcccca
    11281 tttgatatgt tttcccttga taaacatact gatcatcttt taaacgtcca acaaaaggga
    11341 ataaaaccgg tgcatgtctg ccccaatacg ctggatcacc ttgccagata tactctaaac
    11401 catgttcttt tgatttcaga ctgatcaatt ccgcaccgtg ttcagcgatt tcggcgatca
    11461 gctggtcatt ttgaataata acagtcattg ccattcctcc aacttcgact tactttttaa
    11521 aatgggaagg ctggaacaaa catttctatc tgccccagtc cccttacttc taataactgt
    11581 gttttgctaa ctcgtcttta aaacgggaat tcaatacttt taaataagtt cccttcatgc
    11641 ctaacgaacg agattcaatg attccagctg attccaattt tcgtaaagca ttgacgatca
    11701 cagaacgagt gatgccgata ctatcggcaa tattagaagc agtcagacga ccttcatctc
    11761 catccaatgc ttcaaagata gcttgtacag ccttcaattc gctataagat aatgtgttga
    11821 ttgccatttg gacagccgtt gcactccgta catctgcttc gatgcttcga gacttctgat
    11881 aaaggatctg cataccgaca accgtagctc catattctgc taagactaga tcgtcatctg
    11941 caaacgattg ttccatacga gataagataa tcgtacccaa acgttcgcca gctccaaaga
    12001 tcggaacgac agtcgtcaga ccaaatggat atttctctct tgactctacc ggaaatgcag
    12061 taatatcact agtaattggg atattagctt cagttttcat caactgtgcc actgtatcag
    12121 tataagattc aggaaattgt ttctcaacaa acattgtttt gattcgttca ttgttcacat
    12181 cgtgacgttc attataacct agtaatactc cgtcacttcc tatgatatag gtgttgctgt
    12241 caagaatacc acctagtgtg acagccattt tatcgtaagg caaatctgct tgcatgtcaa
    12301 aagtattctt ttgctgcaaa agtgtgttta tcttgcgggt tttcgttagc aagtctgtca
    12361 ttgtcgtcct ccgatttttt ataaaatata gcgacttaaa tcttcatttg ccgcaattga
    12421 gccaagtttg tcatccacat atgcttctgt gatcgttatt tctcccattt gcatatctgg
    12481 tgcttcaaat aatagatctt ctagtagttt ttccaaaatg gtatgcagtc tgcgtgcgcc
    12541 gatattatct gtgtcacggt tgacatcgaa agcgatttga gcgatccgtt caattgcttc
    12601 ttttgtaaac gtaactgaca cattttctgt accgatcaac gcgacgtatt gttttactaa
    12661 agcattattt ggttcggtca gaatcttcac aaaatcttca gctgtcaaat catctaattc
    12721 tacccgtatc gggaagcgtc cttgaagttc tggaattaga tcacttggtt tagacaaatg
    12781 gaaagctcct gaagcaatga ataggacgtg atctgtttgg atgatcccgt attttgtgtt
    12841 tacttgtgaa ccttcgacaa ttggcaatat gtctctttgg acgccttctc tggatacttc
    12901 tccagattgc tgtgatttag aagtgatctt atcgatttca tcgataaaaa taattccact
    12961 ttcttccgct aatttgattg cctcactatg gatatcagca tccttgacta attttgctga
    13021 ttcttccttg atcaataact ctaaagcttc tttgactgtt accgtccgtt ccactttttt
    13081 cttcggtgtc aaagcaccta gagtttcatt taggtcgatc cccatttgct ccaatccatt
    13141 gttcatcatt ggtgcaggtt tcttttggtc ttcaacttca atggttactt cacgactatc
    13201 cagtaaaccc ttttgtagtt gttccaagac tgtacggcga ttgattcgaa tatcttctgt
    13261 cacttcttct tgcggttcag cggtattttg ctgactagca ttaaaaagat tcatcatttg
    13321 ttcaaattga ttagtggatt gtttctgttc ttttttgata ccaggtacca agatttttac
    13381 caaacgtttt tctgcatttt ttttagcact tgtgtataca cggctgtact gctctttttc
    13441 tacgatttgg atcgcatttt ctactaaatc ccgaaccatt gattctacat ctcttccgac
    13501 ataaccgact tcagtaaatt ttgttgcttc gactttgaca aatggtgcat tgacgatttt
    13561 tgccaaacgt cgtgcgattt ctgttttacc gacccctgta ggtccgatca tcagcatatt
    13621 ttttggtgta atatcttgtt gcattttttc atctaactgc aaacggcgat agcggttacg
    13681 caaagcaacg gcaacagatt tttttgctgc gtgctgtcct acgatatatt catctaattc
    13741 acttacgatt tgtcttggtg tttgttttaa ttcatacata tctactcacc tcttacagtt
    13801 cttctacgat aatattatga ttagtaaaga tacagatatc tgcggcaata tttaacgcat
    13861 tttctgcaat ttctttcgca ctcatgtcgt tttgagcata gtttttcatt gctcgggctg
    13921 cagccaacgc aaaattccct cctgatccga tagctaagat accatcatct ggtgtgataa
    13981 cttctcctgt acctgaaaca agaagcattt ctttatcatt cattacgatc agcatcgctt
    14041 caagtttttg catagattgc tgtgtccgcc attcttgagc taattcgaca gctgcgcgtt
    14101 gtagattccc gttatattcg tttagtttgc cttcaaattt ttcttctaaa gtaaacgcat
    14161 ctgcaacact tccggcgaaa cctacaacaa cttctccatt gtagatacgt cgtactttct
    14221 ttgcagtgcc tttcatgaca acagattcac ccattgttac ttggccatct ccagccatag
    14281 caaatttccc gtctttttcg acggcacaga tagtggttga atgaaatggt gattcgacca
    14341 tgtttgtttc ctcctaaatt tgttcttttc cttattttta cgctcttgga tggaaagaac
    14401 gatagttttt ttgtaaactt tcttttgtta catgggcata gatctgggta gtcgataaat
    14461 tggcatgtcc tagcaattct tggactgtcc gcaaatctgc cccattattt aataaatgag
    14521 tagcaaatgt atggcgcagc atgtgaggat ggatatcact atccaaactg ctttttttga
    14581 tcatttgatt caagacatat tcaattccag taggcgtgat tggttttcca tgatgattca
    14641 caaaaacgat atcatgctct tcgtgatatt ttgtcatcaa tacctggcgg ccttccctga
    14701 aatattcttg cagagcatct tgagcaaaag aacctagagg agcatatctc tctttgttcc
    14761 ctttaccatg gatcaacatg acctctgaat caaaatcaat gtctgatacc gacaaactgc
    14821 tacactcact taaacggatg cctgacccgt aaaggacttc cagtaaagcg cggttcctca
    14881 aatccaaagg actatctcct gctacgctgt caaaaagtgc ttgcatttca ttttcataaa
    14941 aaaaacgtgg cagtctcaaa tttttctttt tgagatgaac gtaagaaaat ggattttctt
    15001 ttatcacttc ttctttgaga agatactgat aaaacgaacg taggctggct atcttgcgac
    15061 tgatagaatt tctgctatag ctaggacgat gatcagctaa tgcactcaaa tagacacgaa
    15121 ccgtaaaata ttccgccttc agcagatcag cgtctccaga ttcctccaaa aaatgtttga
    15181 aatggagaat gtcttcttca tatgcttctt tcgttttttc tgaatatcct ctttcgacaa
    15241 tcaaataacg caaaaatttt tgttcccacg tggcttctgt cattttctca ccttcctttt
    15301 gtcccagaga ataatttagc acagcgttta taaaaagaca aataaattgt cagaatttaa
    15361 ccaatatttc taagtttgtt cacaattggc gcaattcctc acattttgtt tgagaaattc
    15421 ttaagaaaaa cgcaagaaaa agagcgagac tatccgtctc accctcttca tggagtttcc
    15481 ttactcattt gggattgcag ttttttctaa ttctgttttt attgcctcat tagcttttaa
    15541 agctcgttgt gcaattgctt catatcttgc ttttttgtct cgaatgcgct ctggcagttc
    15601 aggaaacaga ccatagttgg cattcatcgg ttggaaatgt tttccttccg catgagtaat
    15661 gtagtaagcc atacttccaa tagctgtttc ttgtggaaac tcgactggtt ccatttcttt
    15721 tgctaagcgt gcggcgttga ttccagcaag tagtccgctc gctgcacttt caacatagcc
    15781 ttctacacca gtcatctgac ctgcaaagaa cagatcttcg cgtttcgttg attggtaagt
    15841 cggtttcagc aattctggcg aattcataaa gctgtttcgg tgcatcacac catagcgaac
    15901 gaattcagca ttttctaacc caggaatcat ccggaaaaca cgtttttgct cgccccattt
    15961 caagtgagtc tgaaagccca cgatgttgta taaagaagct gcagcattgt cttgacgcag
    16021 ctggataaca gcataaggac gtttgccagt tttaggatct tccaatccaa ctggtttcat
    16081 aggtccgaaa agcatcgttt tgaatcctcg ctgtgccatt acttcaattg gcatacatcc
    16141 ttcaaaatat ttttctttct caaaagattt aagttctgcc acttcggcat tgattagtgc
    16201 ctcatggaaa gctttgaatt cctcttctgt catcggacag ttcaagtaag ctgcttctcc
    16261 tttgtcatag cgggacttca aatatacttt atccatatcg atcgtagctt tgtctacgat
    16321 tggtgcagct gcatcataga aataaaaacc atctgaacca ttgaatgctt tgatgctttc
    16381 tgctaatgga tgagaagtca aaggtcctgt tgctactacg gtgattcctt ccggtatttc
    16441 agtaatttct tcgtttatca ctgttactaa tggatgctct ttgacttttc gggtaattgt
    16501 ttcagaaaaa gaatcgcggt caacagctaa ggcaccacca gctggcactg ctgtttcatc
    16561 tgccgaagtg attactaccg aattcaatcg gcgcatttct tctttcaata accctactgc
    16621 attagccaaa ctgtttcctc ttaatgaatt ggaacatact agttcagcaa aattacttgt
    16681 gtgatgcgcc tcagtcgatt tgactggacg catttcatat aaacggacag ggactcctgc
    16741 ttgtgcgatc tgccaagcag cttcacttcc ggcaagccct gctccaacaa catttaccat
    16801 tttcaatgaa agaacctctt ttctattatt tttggatgtc ttcttcgtaa tctccattta
    16861 cacagactac ttgtttaccg cctttgactt tcttctcgac cagatatccg tcacattttg
    16921 gacaatttct gcccactggt ttatcccaag aagtgaattc acattctggg aaacgggaac
    16981 aaccatagaa tagtctgttt ttcttagatt ttcgttcaat gacttgtcct tcatgacata
    17041 ctgggcaagt cacaccgatt tcttttacga ttggtttcgt attccgacaa tcaggaaaat
    17101 tactgcaagc ataaaacttc ccatatttcc ctaattttat caccatcgga tgtccacaga
    17161 catcacaatc gaatcctgct ggttcatctt tgatttgaat tttttcgatt ttttcttccg
    17221 cattcgtcag ttctttttca aacggtttat agaaacgatc aaccactttg acccacgctt
    17281 ccgtgccttc ttcaatttta tccaaatctc cttccatttc tgctgtgaaa tggatatcga
    17341 cgatttgagg gaagaattca cagattaatg aattgacaat ttctcctagt tcagtcggct
    17401 caaatctttt ttgtgtcaat tttacatagt agcgtctttg gatcgtatca agtgttggtg
    17461 cataagtgga aggtcgccct accccgtttt cttctagtgc acgaatcaac gtagcctcac
    17521 taaaacgtgc tggtggttga gtaaaatgtt gttttggttc aatatcaaca gattggacta
    17581 catcgccttc ttcaagttct ggaagaatat tttctttctc ttctttgccg tcatctctac
    17641 cttccacata cacttgcatg aatcctttga atttgatttt tgaaccgttc gcaatgaaga
    17701 tgacaccatt ttgttctaat gtcactttca ttgtatctaa aatagctggt gtcatctgac
    17761 tagccacaaa acgtgaccag atcaatgtat aaagtttcaa ttgatcttta tctaaatatt
    17821 gtttgagttc atctggtgta cgaagcacac tcgttggacg cacagcttca tgggcatctt
    17881 gtgcgccttg agcatttttg atctttcggt gatcatgtct agaaaattct tttccatacg
    17941 tatcttcaat aaatgctgcg gcctctgctt ttgctgtatc ggcaatccgg gtagaatcag
    18001 tacgcatgta tgtaatcaag ccgactgttc cttgtttacc caaagtgatt ccttcatata
    18061 attgctgtgc taccatcatg gtttttcttg ttcgaaagtt taacttacgg gcagcttctt
    18121 gttgcaggct acttgtagtg aaaggattcg cagggtttct tttgcgctct ttcttttcta
    18181 cttttttaac ttcgaagtct gttccatcaa ttctagcagt gacttctttt acactttgtg
    18241 catctggtaa tttctttttc ttaccgtcga ttccccagaa atttgctttg aatttcttgc
    18301 gagctttttt aaaattaccg tcgatgctcc aatattcttc tggaacaaat tctcggatct
    18361 ctttttcacg gtcaataata atttttaaag cgacagattg gacacgtcct gcacttaacc
    18421 ctttcttgac cttacgccag agaataggac tgatcgaata accaacgatt ctgtctaagg
    18481 tacgacgagc ttgctgtgca tctactaaat ctacatcgat cgttcttggt tccttaaaag
    18541 ctgctttgac tgcgtctttt gtgatttcat tgaagaccac ccgatttttt tctttcagat
    18601 ccaacccaag taggtacgct aaatgccaag caatcgcttc tccttctcta tccgggtcac
    18661 ttgccaagta aactttttga gctttttttg ctgcggcttt caggcttttg atgacatcgc
    18721 cttttccgcg tatagaaata taatggggtt cataattgtt ttcaaaatcg atacccattt
    18781 tacttttcgg taaatctctt atatggccaa cactggccat tactttatag tttcgtccaa
    18841 gatatttctc aattgtcttg gcttttgcag gtgattctac aatcactaaa tatttatacg
    18901 ccattactga atggactcct ttccattgtc gtgttcctac tattaaaaga ggattcatac
    18961 aaatcgtagc taatcatatc cattcagaaa acgtttgtca agaaaagcaa atgaaaagta
    19021 tggtgttatt ctttaaattc tgccaagata tcttcacccg aaaccgtgca aactgcaccg
    19081 tcttggatca aatgatgaca tccggtagaa tgttctgtta aaatatttcc tggaacagaa
    19141 aaaactgttc ttccaagatc caatgcttgc tgtgctgtta tcagagagcc gctttttttc
    19201 ttggcttcaa agacacatgt tcctttagac aaagctgcga taatccgatt tctcattgga
    19261 aaacaatgct ttttcgggcc ggattgagga ggatattcac tcaaaagaag atgatcattt
    19321 gcaatcacct cttgcaagtc acgattttca gcaggataga cttgctctat ccctgttcca
    19381 atgactgcga ttgtccgtcc accatgagag attgctgctt ggtgaccgta tgtatctatc
    19441 cctttcgcgc agccactgac gatcacatat ccagcttcta tcactggttt tagtaaataa
    19501 taaactgttt ttttggctaa agcagaagtg tgtcttccac caacaacgct taacatgtta
    19561 caagaaagta aagatagatc accattataa aacagtacaa ttggcggata ggtcaagtga
    19621 agcagctgag ggggatattt gggatcaaag caagtgataa acgactgttt cgattggatt
    19681 ttgccccagt tagcgtgaat ttcctgccaa cttttgcgaa acagctcccg gtgtttggtt
    19741 attttaccta tcttaatgat gtcttcaaat ccgatttctt tataaggata ctgcatcatc
    19801 cagttagcaa gtgcccattt tccttgacta ctgattcctt ttgtataagc aagtttcaat
    19861 aaattttctt ctatttgata catcataaaa ccgcctttcg tctctctttc taataagata
    19921 cgtaaaagcg gctgtttttt attgtaaata tttttttaca ggtgcaaaac ttttacgatg
    19981 gattggcgta attccttgtc gttcaagtcc ttctaagtgt tcctttgttc cataacctgc
    20041 attatgcgag aaaccatatc caggataaag cacgtcatat tctttcatca attggtctcg
    20101 atatacttta gcaatgatac ttgcagcagc aatcgacaca gatcgtgcat cccctttgat
    20161 tattttttcc tgctgaatag gcaaatccaa ggtcatcgca tcgatcaaca aatagcttgg
    20221 tacaatggac aatttttcta ctgcttggat catcgctaat ttacttgctt cataaatatt
    20281 cacatcatcg attgtctgct gatcaacgat cccgatcccg attgccaggg ctttttcttg
    20341 aacttgttga taaagaagtt ctcgcttttt ctctgataat tgtttggagt cattcagccc
    20401 taatatgatc tcatttttcg gcaagataac tgcagctgcc acgacaggtc cagctaatgg
    20461 tcctcttcct acttcatcaa tgccggcaat ccattggtga ccttgtgcgt acccttgctt
    20521 ttcaaagcaa aacatttctt catatttttc cttaattaat gcttcctttt tcaatcgtcg
    20581 ttcaaaagaa gccagtgctt gctgcacgcc tttgcgtgga tcatttctta atgattctag
    20641 tgtaggatca tctattctct gtatgctttt caatcgttct ttgatacttt ggatggattc
    20701 agtcatcact tttttgctcc atttcttcgc aacgatccag tgtgtatcgt ccaagttttc
    20761 cttgacgaat atcatgcacg actaattcgc tgccacgatc atagtcttct ttatacccac
    20821 gttttgctgt aatatccatc agtaattccg gtgccaattt ttcttcttgc tcgattgata
    20881 aaccataacg atcgctgatt cttcctgaat aatatcgggc aaaaaagtct aatccgtaaa
    20941 tcgctaaatc atctaaatgg acaagctggt ctttgatcgc gcctgtcagc gctagttttt
    21001 tgccgatttc ttcatcttca aatttgggcc ataaaatccc tggtgtgtct aacaattcca
    21061 actcattacc taaacgaagc cactgctgac cttttgttac gccaggctta tttcctgtct
    21121 gtgcaatttt cttgcctact agtcgattca tcaacgtaga tttcccaaca tttggaatac
    21181 ctaagaccat tgcccgaata gcgcgaggct tgatcccccg agcagcatct cttgcccgtt
    21241 tttctttcaa tgcttcttta gccgcaggta ctagaccttt aattccttta ctttcttgtg
    21301 ccgtgatagc taatgcaaga tggccttgtt tacgaaaata ttcttgccac agctttgtct
    21361 gttgtggatc tgccagatcc gctttattca ataaaacaag gcgaggtttt tgctgaatga
    21421 tctgatctag cattggatta cgagaagata aaggtaatcg tgcgtctacc aattcaaata
    21481 cgatgtccac atatttcatt ttttcagaaa cttctcgtcg ggcttttgcc atatgtcccg
    21541 gaaaccattg gatggtcatt gttctctcca ttcctttcat accggaatat tccccggctt
    21601 tacttgtttt tttctttttc aatataaatt cactttttta ttataaagaa attccatcta
    21661 ttagttaagt gaaagtgaac cagtcctcat tctatcacat cttcactttt ttgatgatgc
    21721 aaatttttat tttggtttct atatatgaat ctaaattttt gacagcgttt cctaaataag
    21781 agatgataga cgaacaaaac agaaaggaga cgaaacaaat gaatagtatg gtttttgtta
    21841 attttcctgt tgctgatgtc aatcgttccg ttgcttttta tgaaaagctg ggttttaaaa
    21901 aaaatgaaga attttctact gaagaagctg gttcaatggt atgggatgac actttttgga
    21961 taatgcttct ctctcatgat ttttataaaa aatttataaa agataaagaa atagctgata
    22021 ataaaaaaac aagtggtgct ttgatttcgt ttagtgttga aaatgcggat gaagtaaaaa
    22081 aatttgctca aattgcaaaa gaaaatggtg gtactttcca cgaagtagat atgggaatgc
    22141 ctgaagaaga aatgttttca ttagaagtcc aagatctaga cggaaataca ttagaacccg
    22201 tatggatgaa gatgtaagca gataactaat ctgacgtttt ttataacaaa aaaacaaagg
    22261 cggatgatac gataagtatt ctccgcctct atttttttta ttcgtacaat ctctttttta
    22321 cagtttataa aagcagtctt ctcacccttt ttttaattgt tagatgccag tgtatgtaaa
    22381 tcaccaatgt gtcatgacac attggtgatt ttatggtatc cttttattat taactgacaa
    22441 aggagagaat attttgaaca ctttgattgc aaaagacgtt tttagattgc ccgaaatgaa
    22501 tgcaagattg ttgtcgacct ctgctctttt attagccctt gatgtcatct tatacaagat
    22561 agcgattggt cctgcttatt ttcaaatcac attcggattt ttaagtatgg cgcttatggg
    22621 ttatctatat ggaccgatct gggctggttt gcttgaaatg ttgtcgaaca ctttaaattt
    22681 cacgatattt ggttcaggaa cgtttcaagc tgcttttctt atcacggctt tccttggtgg
    22741 atttattaat ggcttatttt tatatcataa aaagataaac ctgccttccg tcatcgctgc
    22801 ccaattattg attatgatcc cagtcagcct ttttatcaat tcttggctga tgtctgtttt
    22861 gtttggtgca gatttcaaag taatttttct aggtagattc atcaggaata tgatccaaat
    22921 ccctattcaa ataatggttg tttattcatt tatcaaaact ttagaggcta gaaaagtgtt
    22981 taaacgagct gtttgatttt acgtactaag gtagtaaact gattttggat taaacaataa
    23041 agcgtgcagt ctcaacagac tgcacgcttt attgtttctc actggatcca ttttatttca
    23101 tcaagtggat aatagacaaa tcttgcttta cctagaatat cagttttatt gatcgtgcca
    23161 aacgaacgac tatcttttga aactcttcga ttatctccaa gtacaaaata attatcttcc
    23221 ggaagcttgg attgtcccgt tagctcttgt agtgtaaagt ccgtcgtata ggatgcattt
    23281 gcatgatctg atttcaggtt tttagttaga taaggttcct taactacttt cccattgatc
    23341 ttcaattgat cattttggta gctgacattt tctcctggaa gaccgattac tcgtttgata
    23401 taaatcgtgc catctgctaa ttgaaaaaca acaatatcaa atcgtctgat ctccgaaaat
    23461 ttttccatca caaccatatc accttgcttc aacacgtttt ccattgaatt gccttccact
    23521 ggtaccggga tgaataaaaa tccccgcaac atgaacgcaa ggaatacaga aacaagtaag
    23581 tatttgaata caagccagaa gcgattaata tagcgttgtt tttttgtcaa tctctatcaa
    23641 tccatccctc tttttttcat tctttcttta ttataagaaa aatatcaaaa aaatgcgact
    23701 agatagctga aataaaaata attctttcaa ttaggctatt ggacaaaaaa cttttttgat
    23761 cataaagtac aaaactatct cttatatgtc atgattattt tctaaatatt tttcccatgc
    23821 ttcgtcaaag atatccatgg acggcaaata gtcgacattc agttccagat aattagaaat
    23881 ttcttcgtag ctagagctgt gcttaggaaa ttggatgtct ttaccggctt ctgtagcgaa
    23941 aacagataaa gcatcatgtg tgccaccgcc tcgcagtgtc attaaataat gaaaaaagct
    24001 tcgattcacc cttcgttcct ccaattttct tcgataaagg cagcccggcg tgcatggttt
    24061 cttttaaaat tttcaggatg ctttttataa tactcttgat ggtcttcttc tgctagataa
    24121 aatggttggg ctggttcgat ttttgtaacg attggctgtt cgaaacgtcc actttcagct
    24181 aactgtttct tacttttttc agcaatgatc cgctgttctt ctgtcaaata aaagatcacc
    24241 gggcgataat tgtcaccgcg atcctcgaat tgtccgaaag cgtcagttgg atcagtttgc
    24301 tgccagtaaa gttctactaa ttgttcatat gaaatcaatg ttggatcata gataatctcc
    24361 actgcttctg tatgtccagt cgtttggctt tttacttgtt cataagtcgg atctggtaca
    24421 tgccctccag tatatcctga catcactgta tggataccag gaagcgtgtc gaaaggctgg
    24481 atcatacacc agaagcagcc tcctgcaaat attgctcgtt ccattatgat tccccctcta
    24541 atactactca tccgatgatt ttttattttc ttttgtcttg ctttcagtac tgctggtgct
    24601 agttttgttg gtatttgagt tgtttgaagc atcagaggta tcttttggca aatatagact
    24661 aaaccgaatg tcatcattta ctaagtcgat tttatctgct ttaatataca tcccgttttg
    24721 cattcggaat tgatccaacc taagcatgat agtcagatca tctgtattga cttcgatcca
    24781 atctggtaat ttatagtttc tcttgatcat attcatcaca tctttgattg gcaaactcaa
    24841 tgttccgata gaaagacttc tagcttttag ttggacattt ccgttttcca tcacatacgg
    24901 atcaaaatat aaatagaaat cgataggaaa acctagtact tcgaaagtac cattgagtag
    24961 tgcttcattt tccaaataaa atttatattc aatatcacta tctttttgat actcactaag
    25021 gaaaaagctg atgatttgat tgactttttc tttatttgaa ttgatcgtta agacaggatc
    25081 tccttgtctt tccgtaagag ctggtatttc tgggagttct ggttcccgat tagcaaaaat
    25141 acggctgcct aaaaatacgg tcgaaccaag tactacccca agaaggataa ggaaggcgaa
    25201 tttccagtaa tttgttttct ttgttttgtt tgaagatacg tcagatcttg ttttcatgtt
    25261 tattgatcat cctccttctc taaccattgg ttttgtgtct cgatcagctg atctctcact
    25321 gcatttgcca tcaattgata acctaaatta tttggatgga aatgatcttc ttcatagagc
    25381 acattatttt tgatgccatt tagattttct gtcgtgctag ttgtttgatc tgttccttct
    25441 tctgtgacac ctacttcctg attcaatcct tgataaagca aatcattgat tgggataaag
    25501 tagcagttcg gcattttttt tgtcaccgtt tctgttcctt tgttccaatt gttaacaatt
    25561 gtctgcatgt cagtgatctc aggaaagttc aaataaaatg gattgtagat gcctaaaatg
    25621 taaataggtg cggttttatt gagggatcga aattcttcca atagttctgt gacattttcc
    25681 tgatagtttt tcagtggctt tttaaaggaa ttgaccgtta acccgaataa attattttgg
    25741 ataactttca tcaaatcatt accgccaaca gtcagtgtga taaaatcagc tgttttgata
    25801 ttgtttcgaa tttcttcttt tgtttttact cgctttaaga tctggtcact tcgttcacca
    25861 tttacaccat agttttctgt ttcaaccgcg gtcaaattat aacggtcttt tagatcattt
    25921 gcgacaaggg gaacgaatcc tccttgcttg gtttgatctc ctactccttc agtcagcgaa
    25981 tctccgatag ctatgtagtg aagtttttct tttttatttg gatcttctct cacagtagtt
    26041 actggtttca ttagtgggcc agctttcggt atagaagcag ctaaacctgc atagatgatg
    26101 atcgcaatta ataatgggaa caaaacgagg atcaaatgtc gtttcgttaa tttcattttt
    26161 tcacctcttg tattcgattg gttctaatgt tatcaaaaat tttatcaaaa aggtagtctt
    26221 tatgaaaaaa gaaaacaatt attttcctaa ttttataaag aaaaagagaa taagctgcaa
    26281 gcctttcagg ctgagggctt atcctctttt cgactaatct acataataca tgattgcaaa
    26341 cgcgtcttta ccagtatgcg tagcgataac cggattcgta tgcagtacag ggatatgcat
    26401 gtccgggaat aattcttgca aaccctcttt gaattcttgc gccaattctt ttgcatccgc
    26461 atgtgaaata ccgatttgtc gcacatttgg caattctttc agttcatttt tcaaattatt
    26521 aaaccattta tcgaacgttt tctttcctcg ccccttagtt acagggatta attcagaatg
    26581 ggcgaaattc atcaccacgc gtatgttcaa aacactagac agcaaaccag ttgttcggct
    26641 gattcttcca ccttttacca agttatctag tgtggatatt ccgatgtata attttgtatt
    26701 gtcttgtact tttttgactt cttcaaggat tttctctact gttttacctt cttttgctaa
    26761 ttcagctgct cgaattacct gaaaagataa tccttggtca gtggtatcac tgtcgataac
    26821 cgttactttc gtttttgtta gattactggc ttgtcttgct gcttctactg ttccactcaa
    26881 gccttttgtc atatggatcg aaatcacttc actgccgtct tctcctagct catcatacat
    26941 cgagatgaat tctccaattg gtggttggct tgttttgggc agattttttg ctttcgccat
    27001 catttccata aatctttcgc cgtctagttg gtcatcatcg ggatatactg ttccgtcgat
    27061 catgatggat agcggcatga cacgaatgcc taaagcatcg cgtacatttt tttccatcgt
    27121 acatgaagaa tctgttacga tttttacatt tgtcattatt ttctctctct ctttctttaa
    27181 caagatgcct tattcatgtt aaactacatg taagacaatt tctattttta gtataacaga
    27241 caaataatga tttttcatta attacaccta gtaaaggagc cgttttcatg gaaaaaacga
    27301 aatttaatcg aacgtatgaa atattaaatg aagtgtttaa tgcagtcacc catggaattg
    27361 ggactggatt gagtatcgcg ggactagtgg ttcttttagt aaaaggtgcc cacttgcatt
    27421 cctccctcca tattgtctct tatgctatct atggatcgat gatgattctt ttatttttaa
    27481 cgtctacttt gtttcattca ttgattttta caaaagcaaa aaaggtgttc caagtattcg
    27541 atcatagttc gatttttctt ttgattgcag gaagttatac tcctttttgt ttgctgagta
    27601 tccgaggatg gctaggttgg actttatttg tattaatctg gttgtttgcg attcttggca
    27661 ttatatataa agcgttgacg ttgcataaac gggagagagt atcaaaagta tctacgatca
    27721 tctatatttt tatggggtgg ctctgtgtcg tggctgctgt tcctctttat cactctcttg
    27781 gaatgactgg tgttattttg atggtagcag gcgggctttc ttacacgata ggcgcattct
    27841 tttatagctt gaattctatc cgctatatgc atgttgtttg gcatttgttt gttatgttag
    27901 gtgcaggttt tatgtacttc tctgttttat tctttactta ataaaaaaaa taaagctatg
    27961 gatgggacag ctctaaataa cagctagtct atccatagct tttttatatc gtcaattcgc
    28021 ctgtcccgct ttttacagga ttctcattac gctgatacgt ttcaaattga tgcggataac
    28081 gatttttttc atccatttct ccttgactag tattgatcag tgaccaatca gaccagtcaa
    28141 caggtggaaa ataagcatct ccatcgaata catgatggat gactgtacgg tataatatcg
    28201 tacaaaatgg cagaaattct tgataaacag ctgaccctcc agcgatgaaa aagataccgt
    28261 ctgtttcttt ttccagagca agcacgtctt ccacactatg catgacatgc acgccttctg
    28321 ctttatagga agaatctctc gtcaaaacaa tcgtctgacg attcggcaac ggacggctac
    28381 ccatcccttc gaatgttttt cgtcccatca ctagtgtatt tgcttctgtc atttgtttga
    28441 agaatttcag atcatttggc aaacgccaag gaagacggtc ttcttttcca atcaatccgt
    28501 tttcatcctg tgcccaaatt gcagctaaca ttttctcatc ctttctgatt ctaaacagcg
    28561 ataggagctt tgatcgccgg atgtgggtca tatccttcta attggatatc ttccatctca
    28621 aaatcaaata ctgactgctt ttcctgattc aaaactaaag tcggaaatgc gcgtatttct
    28681 cggctcaatt gttctttcat ttgttcgata tgattgatat aaagatgagc atctcctaat
    28741 gtgtgaacaa attcgccaac cgctaaacct gtttcatgtg caattaggtg agtcagcaac
    28801 gcataacttg caatgttgaa cggaacgcct agaaacacat ccccactgcg ttgatacaat
    28861 tggcagctta gccgtccctc ttgaacataa aattgaaaca tcgtatgaca aggaggcaaa
    28921 gccatgctag gaacatcttc cggattccat gcagaaacga tcaatcttct agaatcagga
    28981 gtctttttga ttgcttcgat cacttgtttg atttgatcga taaagccacc gtcttttgtt
    29041 tcccagtgac gccattgata gccgtagatg tgtccaagat cgccgtatgt ttcagcaaag
    29101 gaatcatcgg ttaggatctt ctcgcaaaaa agcttgctct gctttttata ttcttcattg
    29161 aattcaggat ctgtcaaaca tctatggcca aaatcagtca tatctggtcc attatagtct
    29221 ggacttttga tgtagcgttc aaatgcccat tcatcccaaa tatggttttt attttgtaat
    29281 agatagcgga tattcgtatc tccttttaaa aaccaaagca attcgctttt gatcaatcca
    29341 aaaggtactc gtttcgttgt taacaaagga aacccttttt gcagatcaaa ccgcatttgg
    29401 tatccgaata agcttaatgt gcctgtgcct gttctgtctt ctttacgatg cccttcttcc
    29461 aacaattttc ttcctaagtc aagatacgct tgttccatcc tttttcctcc taatcttcta
    29521 cccattcact caagtattcc caccgtgcca ttttttcttc aagttgtgct tctgtttcag
    29581 atacgtcgtt ttgcaattct tgtaatcgag tgaaattatc tccttgatga ttcatctctt
    29641 cttggagcaa tgagattttt tcttcaagtt ctgcaatctc atcttcaatc gtttcccatt
    29701 ctttttgttc catgtaagat aatttcttct tttcagcagg ttccttgctc tgtatttttg
    29761 gtttcgcttt ttcagcaggc tgctgggtct ctttttgctt cagtaaataa ctggacatgt
    29821 ttccgtaata aagttcttgt tttccgtttc cttggaatac taataactta tctgctacct
    29881 tgtccaagaa ataacggtca tgggaaacag tgatcacagc acctggaaaa cttctaaaat
    29941 aatcttccaa aatcgtcaat gttgctatgt ccaaatcatt ggtcggttca tccaataata
    30001 aaacattagg ttgctgaatc agcagtttca acaaaaataa ccggcgtttt tctccgcctg
    30061 aaagtttgcc tatcagtgtc ccatgcataa atcttggaaa taaaaatcgt tcaagtaatt
    30121 ctgctactcc gatttgactg ccatctgctg tttttgcttc ttcagcagct tcttgtaagt
    30181 aagcaatcat ccgtttatct ggtgccattt cttcattttc ttgtgtataa tacgcaagat
    30241 gaaccgtttc accgatagac attgtaccgc tatccagggg aattctgcca gctagaatat
    30301 taagcaatgt cgattttccc gcaccatttt taccagtaat acctagtctt tcccgagatt
    30361 gaatcaataa atctaaatgt tctagcagtg tttggtcatt tatacggtaa ctgccatctt
    30421 tgatttccag tacttttttc cctaaacgtt gtgtcgctag gttcagctct aaatcatctt
    30481 cttggtttac ttggtacaag ttctccttta gatcttcgaa acggttgatc cgtgcttgct
    30541 gcttggtgcc acgtgcctgt acgccagccc tcatccaagc taattcctgt ttataaagcc
    30601 gcttgcgctt ttcttcttgt tctacttcta cacgatcacg ttctgctttt tctaagacgt
    30661 aagcttcata atttccttta tactcgtata attttccaaa tgaaagttcg aagattcggt
    30721 ttgctactct atctagaaaa taacgatcgt gggtaaccat caatagtgcg ccacggtatt
    30781 gtttcaaata gttttccaac cactcgatcg cttcataatc taaatggttt gttggttcgt
    30841 ctagtaatag taaatcgggt tcatcaatca acacttgcgc cagactaact cgttttttct
    30901 gaccacctga taaagtactg atttttttat ctaattcact gattcctaat ttttgcaaaa
    30961 taatctttgc gttggtatct gtagtccaag catcttcttt attcatttgt tcttctgctt
    31021 tagcataacg tttttgcaca tcagggtctt ctccgttttc ccctagttcg atcaatgctt
    31081 cctcatatgc tcgaacagtt tggatcaagg ggctgtttcc ttgaaagact gcttggagaa
    31141 cagttaattc atcggaaaat tcagctgctt gagaaagata accaatacgg taatcagttg
    31201 gataaaacac tgtttgccga tcaccgtcgc tgctatcgat accggcaata atatttaaga
    31261 gacttgtttt acccgtcccg ttcgtcccaa tcaacccgat acggtctttt tcgtggacca
    31321 aaaaagaaat ctggtcaaac aaatcttttt caccataagt ttttttgagg tcagttactt
    31381 ttaattcttt caacatgttc cctccttatc ttggatattg tatcataaaa atagtgaact
    31441 tcaggaaaat ttttttggta tttgttcttt ttgtgttctc aaaaaaagaa caggaccgat
    31501 tcgatcctgt tctgatatgt tttgtttttt ataaaacttg aaaattttta cctttttatt
    31561 ttattggact cgtttttttt gactttgctc gaaacgcaaa tcggaaagaa aagttatcag
    31621 atggtttgtt catcgtataa gattctaaca aattgatatt cctgcgatag ctgttcgcag
    31681 aaaacgtcgt gattttttca gcaacttcgt attcatcaat gatttttctt tcaagataat
    31741 aacctcttag catttttttc gtaaacagac tatcttgctg gatcatccac gaagaaatca
    31801 aattccccga gttcattttt attccttcaa gtttgcgttg ctggataaaa aattgaacca
    31861 tcgtttcatc ataaatatca gtcaagttat ctaaactttc aataatcaac tgagtatttt
    31921 tttgatatgt ctctctgatt tcttggatat cttcatcact aataaatgtc tgacgatcag
    31981 catatcttct ttgccaaaac attttcggat ggagaacgat tcgcattaac ctacggacaa
    32041 caatgaacca aaagccgata aaagacaaaa tctggcttgt gatagaatgt tcgaatcgag
    32101 aaagaaaacg ggagtaaaaa cgataaccat tagaagaaag cttgcccgaa cgataacttt
    32161 catccaatcc atcccgttcg atcgacaaaa tcagtgcttg tatttcttgt acttcttgct
    32221 tttcatcatc ggttaaatct tccaagtaca gatcacgtat ccgttcttga taattttcta
    32281 tcactgcttc agtggctaaa aattcttttg tattacgatc aatttccttt atttcttttc
    32341 gcaaaacttc aacgacctct tctaaaatca tcaaagcatt caaatccgtg cttttcgctt
    32401 cctctgtttc cgtcagcata ggcaagacaa tgatccctat gaccaaagtc actaagatga
    32461 cacccgccgt cagaaacaaa agcaaagacc gctggtaaaa catcatatta ttgattgaaa
    32521 atggcaaaat aaaaatcgta gccaaactga ctgttccctt tacaccgcca aatgttaaga
    32581 tcaagatctc gttcaactgt tttttgaact ttttactgcc atctttgaat acataaaaaa
    32641 gactgataga aatgaatctg atcacaaaaa gcatgacaga gattaatacg ataatcgcta
    32701 ataaaagacc gtttggatat aaaccgtctc cccatactgg cgagaacact tgcgtcaatt
    32761 cgattcctaa aaatataaat actaatgcat tcagtgtaaa agcaatagtt gtccatgtac
    32821 tgtgggacaa gctcgacaat tctgcatcaa acacagagat tttcctaaaa ccagaagctt
    32881 ggagaatacc tgctgccact gcagcaatga ttccagatac atcaaatgcc tctgccaata
    32941 cataggctag aaaaggaagc aaaagttcga tcagcagata ccctgtgaca tcttgtgctg
    33001 atgctttttc gatcaaatga attatttttt gtttgaacca gacaagcaaa aagccgatca
    33061 aagctccccc aatactagaa actactagac tcatccccgc gtttactgct gaaaaagatc
    33121 cggtgatcag cgcaccaaga gcaaactgga aggcagtaac accagatgca tcattcaaaa
    33181 gaccttctcc ttccaaaata tgcatcgcct ttttagggat attcactcgt ccagaaagag
    33241 aactgactgc taccgcatct gtcggaccta acgctgctcc aaaagcaaag caggcagcaa
    33301 gtgggacact tggcaacagg aatgataacg tcgcacctac cccgacaagg gtcaaaatga
    33361 ctcctccaaa tgccaagaac aaaattgtcc cgaaattttt gagaatcgaa gaaatatctg
    33421 ctttttctcc ttctctaaat aacaatggtg caataatcat aactaagaac aattcaggtt
    33481 caaaatcgat tgattgtccc cactctgtca gacctaatac cactccaagg aaaatttgga
    33541 tcagtggagc tggaatcatt ggcaaaatgc ggctagctat gttcgataag gtgatcgtta
    33601 ctgcaaatac aataactaac tcagcaaatt ccatcgtcca tcccctttat tttttatttt
    33661 gtgtaagcat ttttaaaaat tgttcttttt tctttaaaat tttggtttcg actacttcaa
    33721 cctgtgtttg gttcccctct aattcacact gtacacgttt tgactccaaa cgaagcagat
    33781 catgtttcag acgttccaac tgacgataca tcaatatatt tcttttacgc aacttacaaa
    33841 gtatttgacg gcggtcatcc atcgaaaggg cgccatagcg atcatgcaac agtttttttt
    33901 gttgttcaaa actctctctc attttccaga tctcctatac acaaacttcc atcattatag
    33961 caaagttctt tttggatgaa aaatatttca ttgtttcatc gtttattgct agttagaaat
    34021 ttccataaat ttttcaacgc ctcattctat tccaaatagt atcagatatc aagtaaaact
    34081 taagaaagat aaaagtttct gacaaaagga tgtttacttt cccattcctg cctttcttgt
    34141 tcttttatca caacaaaaaa atggggctgc gacaaaaatc ttgtcacagt cccttattct
    34201 gaataaacgg tgtggaagag acagcctatc agtagtaata agacacatct ctttcacagc
    34261 ctcatttttg cgtaaaaatc gatttattcg aaaaattccc cgtatacatc ttctacacgt
    34321 tgatctggat tgatcacgat atacaagtta cctgtttttt cgctgatttt tgttgattga
    34381 tattgattgt ctaatccttc tgttccttta tctagatatg ggaagtacac aagatcaggt
    34441 gtgccatttc ggtaaaggat ttccattcgc tcgatatctt cgtatttcaa tgcgcgatta
    34501 aacatgccca tttccagtcc gcctaggttg atatcatgtg tcgcaactgc gtccccttca
    34561 tggaagattt cgattttaaa tccagcacac ggatggattt caacaaattc actgccattg
    34621 attcgtccaa aacttgttgt gatttgtttg atccaaagat cgccgatatg acggcggttg
    34681 atcgtccagg tttctccatt tctaaaataa aattttagcg ctgtcatttc tttactcatt
    34741 aaaaagactc ctttatgatg taatttattc ttgtactacc tcagtttagc acaaatttat
    34801 gtaagtggtc acatcttatg ttttttcttc taatgacgta tctgcccact ttaagtagcc
    34861 aacaaagctg agcagataca gtctagtgtt ttttaaatca atttttcttt cgataaaaaa
    34921 tccaaaacct gttttttatc attttttatt ctttgttcta cgacggcttt ttcagctaat
    34981 tgcaaagctt cactaaccca cttaccaggt ttttttccag tttcttctag caagatttta
    35041 ccatctactg ctaattcgct taatgacttg ataggtaaac gatcatagga ttctcctacg
    35101 gcatccatct gaggtgtttc gtcaaaatag atcatcaatt cttctacttg cagtgcatgt
    35161 tttttgccta agaagtacaa ttcgaatggt tccaaagctc cagtttctcg ttgtcgtagt
    35221 ccttggacaa gtgcttgaac gtctttaatc atttggttag aacacttcca cgatttcaaa
    35281 aaggaacgaa tctcattttc ttttagatcc agttggctga tcaataatgc ccatgcttgg
    35341 ctctcttctt tcaatgggac ggatggtaat tcagcaaaac gtaaaagtgc ttcgccttct
    35401 tctttcaaac cgggacagca aatgtagcac tctgtttcaa taaatgcatg gattcccgcg
    35461 gcccgttcat ttcccaagag catcttgatg aattccacat ttatacgttc tacagatatt
    35521 ttacctaata gctcgtggtt ttctttgatg gcgtagaagg tttcttcctc taatttgaac
    35581 cctagttgac tgacaaaacg caacccgcgc atcattcgta aggcatcttc atggaaccgc
    35641 tcatgaggat tgcctacagc tcgcaagatc tgttcttcta gatcttgcag accatcgaac
    35701 aaatcgatga tttctccatc ttctttcaaa gcaaacgcat tgatcgtaaa atctcttctt
    35761 ttcaaatctt ctgccaacga acgaacgaat tcgacatgat ctggtctacg gtagtcctga
    35821 taagttgatt ccgtccgaaa tgtggtgatc tcatattgtt catcattttc taataccaac
    35881 acagttccat gttcgatccc aatatctatc gtttttggaa aaagctgttt gatttcttct
    35941 ggaaatgcgc ttgttgcgat atccacatca tggattggct ggttcagcaa gatatcacga
    36001 acacttccac ctacaaaata agcttcatat cctgcattct ctaatttttt cagaactgga
    36061 attgcctttt ggtattccag aggtaattgt tttaatttca tttttccaac ctttcctgcg
    36121 tgtttcttgt cctgagataa agactcagtt gcatggtgac tgtttgacaa gtccgtttca
    36181 gagtcttcaa ggccaggacc acttcatctt gtttactcgt ccttacgctc aaagcgatta
    36241 tggtctcttt gattgaattt ttccatgttt ttttcaaaaa cttcagttaa gtcgatattc
    36301 aatgaattag ccatgatcat cgtaacgaat aaaacatcac caagttcttc tgctaccgag
    36361 ttaactggtt cagaagcctt tttgcttttt tctccataag tgtggttgac ttctcttgcc
    36421 aattcaccta cttcttcagt taatcgagcc atctgagcta atggagagaa ataccccacc
    36481 ttgaattgct ggatatattc atccacttcc tgctgcatac tgtataaaga acgcttttta
    36541 tccatctttt tccctccaaa actatcttac caaaacttta acgcaaatcc tagataagag
    36601 caattgagaa aatcgaattt ccatgctaca tttaggaagg aatttctaaa tggaaattct
    36661 gtgaaaatac ataattgaaa gctggaattc atatggtcaa acgaaaaaca gtaaaagaag
    36721 ttctttttat cattatagga acaagtattt atgcatttgg tcttgtttac ttgaacatcg
    36781 ctaatcattt agcagaaggg ggagtctcag ggatcacctt gatcttaaga gctttatttg
    36841 gtattgatcc agcttatacg actttattaa tcaacatccc attgatttta ctaggaggaa
    36901 agatcctcgg taaacgttcc ttggcctata ctgttctagg tacactttct ttgtcattct
    36961 ttttatggat ctggcaaagg gttccactag aaatcaattt acagcatgat ctactgatcg
    37021 tttctctact tgcgggttta gttgcaggtg taggaagcgg gatcgtctac cgcatgggtg
    37081 gaacaacagg cggcagtgat attatcgctc ggattttaga acaaaattac ggcatcacta
    37141 tggggcgttc cctattggca tttgatatca tcgtcttact tgcttcatta acctatatcg
    37201 acttgaaacg tatgatgtat acgttgattg tttcgtatgt tttcagtcgt gtgattgatt
    37261 cgatcttaga tggcggatat tctgcaaaag gtctacttgt cgtctccaac aaaagcgaag
    37321 agattgcacc actactgatg accggtctgc aaagaggtgt gactttcctt tctggagaag
    37381 gcgcattctc tggtaattca aaaaaaatca tctatatggt cgttagctct agtgaattaa
    37441 ctgaagctaa gaggatcgtc cacgagattg acgaaaaagc tttcctttca atcatcaatg
    37501 tgcatgaagt ggaagggcaa ggctttactt atttgaagcc acagacaaga ttactgaaaa
    37561 aactataaga aaaaagacaa agataggcta tgaggaaaga ttccacatcc agctgacctt
    37621 tgtctttttt agtactatac tatttttaac taattgttat gttcacttac ccggttactt
    37681 ttcttttgtg tctgcaaaag gatctttcgg ttaacttcat cagataattt caaaaaagca
    37741 tccctatctc catcttctag tgcttgatcg atttgagcat aaagcatttt tagttttgcc
    37801 tcttcttctt tttcttgaaa atatcgttca attaattcag tatcttcttc actgactgaa
    37861 gaatcccaag aagaaaatgg attgtcttct aagatagata gatattctct tgtctgccaa
    37921 gcattttcga atatacattc cacatataaa ggctttttcc aattcaaacg gatttcatga
    37981 aaaatctggt ctgtgtccgt aaattctttt ccctgcaaaa acaatttcat gggttcccct
    38041 tcaacagata gatcactgat ctgcagtcct cgatccgcaa aattcgcccc ttcaacaaag
    38101 tggacattgc ttaaaatcgc ttcatgattg gcaagataat tcaagatcca aaaaacttct
    38161 cttcggctaa aagatacatg attgaccatc cacgttaaaa aattcttctt ttcccgtaca
    38221 tcaattagca tttttctcac ctctctgtta ggtcatctaa taatgacagt gcctccatat
    38281 ctcctggttc aagtgttaaa taataagtca ataattcttg cgctcttttc agctgccctt
    38341 cttctcttaa gaaaaaggca tactctttca agaaatctgg ttcatgtgat agttcatgat
    38401 aagcctgttc atagtgtaca gctgcaacgg taaagtcttc taattcatta taagcatggg
    38461 ctaggttcca ttctgcataa ggattagcag tttcttccat ctgattgatc acttgtatca
    38521 catcttcata tctttcatca tccaagtaaa gattacttaa agtaagtaat gtttcatctt
    38581 gtttgtctcc tacttctaaa gctttcagta aaagttcttc tgctcgctgt ctatcatgca
    38641 gacggaaagc attttccgag gccaggtgat aaagttcgac ttggtaagga ttttctttga
    38701 ttccttcttc taacactgtt tgtgcttctt caatcatttc ttcttcttga agagcttgtc
    38761 ctagatatag atataacgat tgataatgcg gattaacttc tcgaagttct tccagatagt
    38821 tgattgcctg ctgattttct ttcaattgaa gattcgtaaa agctagctgg aacagtcgat
    38881 catctgtcct ttcttctttc agtgcttctt ctaaaggtac gatagctgct tcgaattctc
    38941 cttgcatact taaggattgt cctaaacgtt cctgcatcga gattcctgaa atttcattta
    39001 tcccgcttgc caacaatgtt tcataaacca ctgctgcctc tttgaaacga tcaactgaaa
    39061 aatacaattc agctagcgca aactggatca atggttcatc aggaagcaac ccagctgctt
    39121 ctttcaattt tgcttcactc acttcaggga ttccgataac ttggtacaaa tcagctatcg
    39181 ccaataagct ttgcggatag taatcacttt gcttatccac tttttccagg tattcaaaag
    39241 ctaagtcgat ctcattatct tcgattgcaa tttctgctaa cgggatgttc agtccgtcat
    39301 tttctgggta ttggttgatc agttgttcaa aaatctgttt tgcttcgatc aaaaagccaa
    39361 tagccagcaa ttcttcgcct agttcagcta atatatctgc tgggtcttct tttaatgctt
    39421 tttctaaaaa gatttgtgct tgtgacaagt cttcatctga taaagcttgc aacatttttt
    39481 cgctatttgt attcatttca attccctctt atctcactga cgatttgttg aaataccgtc
    39541 attgttctca ataattcttt ttcgtttgga tgttggtatc cgtctattat acctattttt
    39601 tctaaaacaa gtattttttt gcttttttct gccaaaaaaa gaactttttc caaatactcc
    39661 atctttgaaa tctgttccag ccagtggatt ggatagccaa gcaagtccag ccacacaaaa
    39721 aagtttttta gttggaaatc aaaaggatat gcttggagat tccacacgaa atgaaacatc
    39781 attcctaaaa attttttcat actagcagaa agtagatggc cattttcggt cagataaaaa
    39841 gcttgttcaa acagtttacc ataatttgac aggttttctg cctctaattg ataaaaatag
    39901 atcatttctt cgatcatccc ggcaaacggc cgagtcgtta attgcttttt atttggatag
    39961 tttttataca gattttgcaa aaaacgatga tcacaaatca ttccgcaaca gataaaagtc
    40021 aaaaagtcaa tctgctttcc gtccacctgc cgctcgctga ttgtttgatc ataaacgatg
    40081 cgttctggca gattgatggt ttgcagcact acacgatttg gtagctgcaa aatcgtccgc
    40141 tgcggtgtta atgctcggct tagagaacgg atcgatacag ggatcatcca tagtgatgaa
    40201 tcaagggttg tatgtttttg aaaaaaacct gccagatcca tgatcccttc atttccaaat
    40261 ccgatgataa acatggattg attttcggga tagcgtttga cgaaatcaag caggttcttt
    40321 aattctgtta aatgattgca ttgagtgttg gcacaaatat accagtcatt gtttgcttga
    40381 tttgcaaata acgggttgat cttatctgca aacaaatcat aatagcgctg attcgttaaa
    40441 aataagattg gctgatcaat ggaaagttct cttagctgac tgactagcgt ttctccgtaa
    40501 aacactttcg tcaacgcatc ttctacttga taatcaagtt ccatacgcat ccctcctctt
    40561 cttattttta ttttaccatt agtaagcaaa ttctagaaaa gataagagca aagctcacct
    40621 cataacttgc aaataaataa aaattaaata aaatacatgt gttttctatg tatgacatac
    40681 ggattcggaa tctctgtctc ttatttattc cttttctgct aggaaataaa aagaaaagca
    40741 cttgtagccc tagagccaca agcgcttttt caactgtctt atttaacagc gtcttttaat
    40801 gctttacctg gtttgaatgc aggtactttg ctagctggaa tttcgatttc ttcaccagtt
    40861 tgtgggttac gacctttgcg agctgcgcgt tcgcgaactt caaagttacc aaaaccgatt
    40921 aattgaactt tttcgccgtt agctaaagca tcttgaatag ttgaaaatac agcatctact
    40981 gctgcagttg catctttttt agttaaatca gtagctgaag caactttttc gattaattct
    41041 gctttgtttg ccatggatag attcacctcc tccacaagag atatgataga aatatcattt
    41101 agttaaaact ttctgagtgg tccagaagtt taaaaagaaa tattgattaa cttcaatatt
    41161 ctgttatcac gatatcatat gaaccgctta atagcaaggt ttttaccaat tttcacatgc
    41221 tttttttagt catagaaacg taacaaacga aactgtcttt ttcttttgaa aaaaaaacgt
    41281 cgatatggta aaatgcctat tttctcctac gagggataat tcggatggac gtcccttcaa
    41341 aagtaaatgc ttttctgatt tgattttcta agaaacgttc ataagaaaaa tgcatcaatt
    41401 cctcttcatt gacaaaaacg acaaatgtag gtggtttgac agcgacttgt gtagcataaa
    41461 aaatcttcag gcgttttcct ttatctgttg gtgttggatt gattgctaca gcatccataa
    41521 tgatatcatt caacacagcc gaagagatac gaagattctg gttcatactt acttgttcga
    41581 tcatttcagg cagacgttcc aaccgttgtt tagtcactgc agaaacaaaa acgattggag
    41641 cgtaatcaag ataccggaat tcatcacgaa tctcttgttc gaaatcacgc attgtattgg
    41701 tttctttctt tacaagatcc catttattca cgacgataat aattccacga ccagcttcat
    41761 gggcgtaacc cgctactcgc ttgtcttgtt cccgaatccc ttcttcagca ttcagtacca
    41821 tcaaaacgat atcagaacgg tcaatggcac gcattgcacg catcacgctg tatttttcag
    41881 tattttcgta tacttttccg cgtttacgca taccagctgt atctatcatt aagaatttct
    41941 gaccactttc agaaacgaag tgggtgtcga ttgcgtctcg ggtcgttcct tcaatatccg
    42001 aaacaattac acgttcttct cctaaaatcg cattgatcaa agaggattta ccaacatttg
    42061 ggcgaccaat caaactaaac ttaatcgtgt cgtcgtcctc ttcttcagtc tcttcagaga
    42121 aatacttcac tgcttcatct aaaatatcac caatccctag tccatggctt cctgaaacag
    42181 gataaggatc gcctagccct aaggaataaa attcatagat atcgtttctc atttctggat
    42241 tatctacttt attgactgct aaaatcactg gtttattact tctgtataaa attttcgcaa
    42301 ccatctcatc tgcgtctgtc acgccttcgc gtccgctgac tacacaaatg attacatctg
    42361 cttcttcaat cgcgatttct gcttgatgct tgatctgatc cataaaaggt tcatcgccaa
    42421 gatcaattcc gcctgtgtcg atcacgctga attctcgacc taaccattca ccttttgcat
    42481 aaatacgatc acgagttact ccaggagtat cttcgacaat cgagatacgc tcaccagcga
    42541 tgcggttaaa aatcgtagac ttaccgacgt tcgggcggcc aacgattgca attgttggat
    42601 ttgccatatt atttcctcct cacaagtttc caatctcact tagtttaacg aatgcattgc
    42661 caactttcaa gcatagattg tcaattaaac gaagtatttt tattccttat tgaaatacta
    42721 atcgcttttc aaaagaaaaa ctgagacaaa agcagctgct ttcatctcag tcacagatac
    42781 aaaatcaatt atttttcaga atcatcggtt gctttttctt ctacttcttc tttcaacgca
    42841 tcacctaaaa tgtctcccat agtaaatcca gtattttctt ctggtaattc ataagactca
    42901 acattttttg gttcttcttc tgaagctggt ttttcttcta aagctttgat actcaatgca
    42961 atacgatgtt cttctggatg gatctctaat actttcactt tcacttcgtc accttcatga
    43021 agcacttcat gtggtgtcgc aatatgttta tgtgaaattt gagagatatg gactagccct
    43081 tctactccag ggaacacttc aacaaaggca ccgaagcttg tcaaacgttt aactgttccg
    43141 tctaatactg tgccaactgg agcttttgta tcgatttctg accaaggtcc aggctgtgtg
    43201 tctttgattg acaaggaaac acgttcttgt tcaggattga tagacaacac tttgacattt
    43261 acatcatcat tgacagccaa tacatcactt ggttttgcta catgactatg tgaaatctct
    43321 gagacatgga ctagaccgtc aattccgcct aggtcgacaa atgcgccaaa atcagtaaga
    43381 cgtgcaacac gtccactgag aacatctccc tcatggatag ttgaaagaat ttcttttttc
    43441 ttttcttctc tttcgttttc cactaccgct ttatgcgata agatcaaacg attttctgat
    43501 ggttcgattt cgatgatttt aaagctcaat gttttgcctt tatagtctga aaagtctgca
    43561 acaaaatgat cttccaccat tgatgcaggg acaaaaccac gtacaccgac gtcaacgact
    43621 aaaccgcctt tgacaacatt tgtaacaggt gcttcgatga ccttgccctc ttggaaatct
    43681 ctttcgatat cttcccaaac ttttttagca tctaaacggc gttttgaaag tagatagctg
    43741 ccgttttctt tatctttgcc gatcgtactg ataacgacta aatctaatac atcaccgact
    43801 tttactactt cgctgatatc ttctaccggc aaagttgata attcttttgc cggaacgact
    43861 ccttcaactc cagccccttc gattccgaca atcacttgtt tgtcttcaat gactaaaact
    43921 tcgcccttaa caacatcgcc aacttttact tcttgtacgc ttttcattgc gtcttccatt
    43981 gactggttgt tctcttgatt ttgatcaaat tctgtcataa cacttgtcct cctaaccaac
    44041 attctgccgt aagtacggta ctgtttttag tttaattgat tattcaaaaa aaataaagcg
    44101 atttcgttcg ttttccttga aaaaacaatt gtttttcatt ttttttctta atttaaaaaa
    44161 gaagataacc ttttttcaaa acaacagctt ttattgctgc aacgacttct ggaatcgatt
    44221 tgccagtcgt atcgatacga acagcatcct ctgcttgctt cagcggagag acgtcccttg
    44281 ttgaatcata atggtctctt tcctgtatct ctttcgtcag tgtttcaaga tcagtcggaa
    44341 ttcctttttc ttggttctct ttgaaacgtc tttctgcacg ctcacttacg cttgctacta
    44401 aaaagatttt cacttctgca ttcggtaata cagctgtccc gatgtctctt ccgtccatga
    44461 caacgccacc gcgttcccct atttttcgtt ggacagcaac taattcttct ctgacttttc
    44521 catgtgcaga gacttcagaa acagcttttg tgacatctgg ttgacggatt tctaaagtca
    44581 catcttttga tgctatgaat acatgctgtc catcttcttg ttgcgaaaaa gagataggat
    44641 actttttgat cagcgaaact agcccttctt catctgaaaa agatacattg ttcgtaatag
    44701 ctaaataagt tactgcgcga tacatggcac cggtatctgt ataaatataa ttcaattctt
    44761 ttgctaatat cttggcaacg gtacttttcc cagatgaagc aggcccgtcg attgcaatac
    44821 tgatttgtgt catgatataa ctcctttttc tcagaaaaaa agccgcaaag cgcggctatt
    44881 attatttaat tcttaattct tgaccagggt aaagcataaa gttgtttgga tctaagccat
    44941 tcaattcaaa caattgatca gtggtgatcc ctgcacgctc agccacttgt tttggacctt
    45001 cgcctgcttg aacagtcgtt gttgaaccgg ctgcagctga tgaagaaggt gttgtttctg
    45061 ctgcattgct gcttgaatcc cctgcttgat aagttgcctc acttgaggaa gtcggtgttg
    45121 tctctgctgc aatactgctt tctgacgtta cacctgtatc ttgagacaca gtagattctt
    45181 ccgcgattgt actagattct actgttgaag tctcagttga ctcctctttt gttgtacttg
    45241 tagaactttc tactactgat gtcgaagttt cagaagaagt tgctgctgtt gcattgttgc
    45301 ttgatccatt tctgatccag aaataagcac ctgctggaat agcgatacat aagaccagta
    45361 aaatcaaaaa tagcgaaaga aataatgtat ttcctttctt ttgttgtctc tgagaacttc
    45421 ttgatgaaac ttcatcatct gtatcgtaga ttggttgttc ccatggttct tgtgtttcgt
    45481 tattttctgt agattgatgt ctatcttttc tgctcactgt tttacctcct aaactctatc
    45541 gtgtgtatta taccatagga aaaagtgtct tgtcctaaaa aatcaatgtt ccatgttttt
    45601 tttaaataaa tttagtaaaa tcgtttccca atgaagttgt tcagtcttag aaaaaagctt
    45661 cctctttggc agaagttctg cttttgctcc atcaatatca caacaattct gcggcttttc
    45721 ggagagtttt tcgccaaaat aagctaagat gaattttctg cggcagtttt cttcattaat
    45781 atagcgaagc atctgctgca ggcggaattc tttttctttt tcctgacgtt tgagccgttc
    45841 cacccaattt tctggttttt cagactgctg gatcagctcc atccattttt tttgcagctc
    45901 atcaaaggga gcttgctctg tggcatattc caaataaagc tcaaaacttt gtcgctgttc
    45961 tctagaaagt tgattaaaga aataatggat acgttcatcc ccactttgat aaaaaagaat
    46021 tgctgcactt tcttcctgat ctctgcctgc tcgaccgatt tcttgtacat agttttctag
    46081 actgtctggc aaatcataat ggaccacata acggatatca gatttatcga tccccatacc
    46141 aaatgcattc gtagcaatga gaaactgcag ctgttttttt acaaactgct gttgaagttg
    46201 tcttctttga gctgcatcaa gaccaccgtg atagtatcca actgtaaaac gttccctgaa
    46261 taaatgatac aatcgttcga cttctttttt tgttgcacaa taaatgattg ccgctccttg
    46321 tgctctttcc atgaagtgtt caagttcttg ttctttttgc tgcgttttcc gaacaaaaag
    46381 cgagatattt tgccgattga ctgattgtct gatctctttt ggtggtcgat caaatagtac
    46441 atgacgtata tctcttgcta ctaaatctgt tgctgtcgcc gttagtgcca gtgtgaccgg
    46501 gtttcctaaa agctttcgga tttttcctaa ctgttgatat tctggacgga aatcaactcc
    46561 ccattgagac acgcaatgag cctcatctac tacatataac gcaatatttt gttttttcag
    46621 atgctctaaa agagaaggat tagtcaacat ttcgggactc aaaaacaaaa atttatattg
    46681 cgatagatga cggaggacgt atcttctttc agattgcgaa agtaaactat tgaaagcagc
    46741 tactcgtttc tcccctcgtt tttgtaattg cgtcacttga tcttccatta gagaaagcag
    46801 cggtgacacg atgatgacca gaccttccat taaatacccc gtcaattgat agcaaaggct
    46861 ttttcccgtt cctgtaggca aaatagcgag agtatcttct ccatcaagca acgcctgaat
    46921 aatttcttct tggccgggac gaaatgaatc aaatccaaaa tattttttta attcatttat
    46981 taacattctg tatcgcttcc tttctcaata gatagatttg atacaaacga aattctccat
    47041 aatctaattg tccatctaca ttccattcgc taaagcgcca ctctcgcacg gaatccttat
    47101 ttggttccaa acgttcgatt gtttctgcag agagaatcca ctcgtatgga aaatcatcaa
    47161 aaaacaaggc ccactcaata agatgatctg ttactgttcc tctttttaaa tgacggattg
    47221 ccatgatctg gtcaattgtc tgaccattca taaacatctg cttggtgatc atcatgcttt
    47281 gattgaaatt ttgctgtaat aatgaatcga tcaaagcgtg ccaaagcgaa tctggccgtt
    47341 tttcgatttg tgctaacaaa aggtggatac atctgctttg ttgcagataa accgctgact
    47401 catcattgtt ttttgccagc tgataaggaa gcagccctgt tcgctcattt ccagataatt
    47461 ggttagctaa aaaatctgct gcttcttctg gtatctccga aaacagctgc accaaactct
    47521 catacgcagt atcaatcaaa atttcacgtg gcaattggct ttcactaagc cattttttta
    47581 gttggaaagt ataaaaagga cttgtctctg ctggaagata gtcttgaata cccgttgcca
    47641 gattagaaat tacttgaaca gcaaatttaa tgagccgcca actagtttcc cctgttctgc
    47701 catagcggtc aaaacgcaaa ccagtatgtt ctatttgatg ttcggacaac atatcagccc
    47761 ctgcaggcgt tagctttgct tcattttcat ttatttcgat ccaaccttgc tgcgcaattt
    47821 tttgcaaaat ttgataaaac ttatcctgct tcaattctgg aaaactgtta tggataaaca
    47881 aaagttcgtg ggtaaatcca taaataagta ctgaagtcgt tcgttttccc acaaggagtt
    47941 ggtaaagaga actaatccgc agcttgtgtc ctgatgaaaa tagcgataaa ataaaaacaa
    48001 tcatattatt ctccttaagt taggatggtt agaaatgtca ctactatgta aacttgtacc
    48061 tgaacgctgt attgcatgcg gtctttgtca aatcgaagcg ccagatattt tcgattatga
    48121 tgaagaagga atcgttttat ttgcccaaga accagaggct catcaacagt tcgtgatgcc
    48181 ggaagaagaa acatatgtaa aaaaagcgta tcaaaaatgt ccagttcgag cgattttatt
    48241 agaaaataaa atataaatat aaacgaaact ttaaaaacgt acactaagtt ctctaaactt
    48301 gaaaataagc cgggatctgt ttctcttgtt ccgtgaaatc ccgacttatt ttgaaagatt
    48361 catttgctat ctatagttaa ttgactagtt ttttcacgga ccctatagga cccatttata
    48421 ttatttcaac tttttcagcc agttttccaa tgtattgacg atcacagttt gctggtctgc
    48481 gttagaaatt tctgcctctc catctccttt ttgctcacca tagctgccaa aacctccatg
    48541 atttccgcca ttgattgata catactctgt tctttccggt aaatactgct tagcttcttg
    48601 ataagcttcc tgattcaata ctttatcttt tgtcgccgtg atcgataaaa caggaacagc
    48661 tgattctgtc aatgttcctt ttttgtcggg ataacttgcc atgaagaata ccccttttaa
    48721 atgtttgtct gaatgattct tattgacata acgacttgcc attacgccac ctaatgaatg
    48781 gccaccgatc acataatcgg aatttggaaa atcttctttg accttatccg ctttgtttcc
    48841 gcccatcact gctagatcaa gtggcatttt gacaatagcg actgaaaagc cgttttttgc
    48901 taaatcttgc gcccaaatac tataactttc tggctctacg agagcaccag gataaaaaat
    48961 aagtaatgga gactttccat gattcttttt ctctccttca aaaaataagt aatcttcttt
    49021 gttttttcct tcttttacca acacttgcgc ttctgacgat ggttcgtacg tattttgctg
    49081 tatatagtaa taaccggctc caccgagtaa tcctaaaatg atgactaagc caattagtat
    49141 tcgtctaaat attttcactt tctcccacct tttctatctg agtgacccct ttttcttaat
    49201 caagtatgat tataccttaa aaaaataaga gagaaatgca aactttccag catcaaataa
    49261 aaaaagacgg aatatccatt tgaatatccg ccctttttgt atgtttattt tt
//
LOCUS       DACGLE010000014        43165 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0534
            SAMEA3304449-rid9560453.denovo.015, whole genome shotgun sequence.
ACCESSION   DACGLE010000014 DACGLE010000000
VERSION     DACGLE010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304449
            Sequence Read Archive: ERR985064
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 43165)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43165)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702225.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702225.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 20:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,045
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 27 of 67
            Pseudo Genes (incomplete)         :: 25 of 67
            Pseudo Genes (internal stop)      :: 19 of 67
            Pseudo Genes (multiple problems)  :: 4 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43165
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304449-rid9560453.denovo.015"
                     /strain="VREN0534"
                     /db_xref="taxon:1352"
     gene            complement(105..629)
                     /locus_tag="IXT20_000916"
     CDS             complement(105..629)
                     /locus_tag="IXT20_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ4859383.1"
                     /translation="MSEEVGIILREAVPGDAKDILLMMGQVNKETEFLVLDEAELLLP
                     PETLEEELDYIYESNNNLLLLAIYEGTIIGTASVKADSQFRLSHVGEVGISILQEYWG
                     MGLGTLMLEEIISWAKEMGVLFRLELDVQVRNERAVHLYRKMGFQIEAVMPRGARTDL
                     GEFLDVYKMSYLIE"
     gene            complement(622..1095)
                     /gene="tsaE"
                     /locus_tag="IXT20_000917"
     CDS             complement(622..1095)
                     /gene="tsaE"
                     /locus_tag="IXT20_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HAQ4859384.1"
                     /translation="MIELSGLTMTEQFAEAIGHSAMPGDNLILTGDLGAGKTTLTKGI
                     AQGLGITQMIKSPTYTIIREYSQGRIPLYHMDIYRVAASGADLGLDEYFEGDGLSVVE
                     WGNLLEEALPEDYLELILEKSDTDLEKRYVKLISYGEQSETFKQRILEKWAENHE"
     gene            complement(1218..2198)
                     /gene="pta"
                     /locus_tag="IXT20_000918"
     CDS             complement(1218..2198)
                     /gene="pta"
                     /locus_tag="IXT20_000918"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HAQ4859385.1"
                     /translation="MELFDSLRFKIVRRGIKIVFPEATDARILGAAARLKAEELVEPI
                     LVGNEEEIRNAAHARGIKTSSFTIISPDNYDKWDEMVDTFVERRNGKASKEQAEKILK
                     DVNYFGTMLTYMGIADGMVSGAIHSTGDTVRPALQIIKTKPGISRTSGAMIMVRGRDQ
                     EKYVFSDCAINVNPTAQELAEIAVDSAKTAELFDIEPKVAMMSFSTKGSAKSPEVDKV
                     VEATKIAKKLAPQYMIDGELQFDASYVPSVAQLKAPDSEVAGQATVFVFPELQSGNIG
                     YKIAQRFGNFEAIGPILQGLNKPVSDLSRGANEEDVYKLSIITAAQTLID"
     gene            complement(2351..3031)
                     /locus_tag="IXT20_000919"
     CDS             complement(2351..3031)
                     /locus_tag="IXT20_000919"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HAQ4859386.1"
                     /translation="MKEIIHNSWQEVLSSEFSKDYYLHLREFLKKEYASQKIHPDMYH
                     IYEALELTPYEEVKVVILGQDPYHGENQAHGLSFSVQPGVKIPPSLRNIYKELYDDLG
                     IAPVQHGNLVSWAKQGVLLLNTVLTVREGQAYSHRGKGWERLTDTIIEKLNEREKPIV
                     FILWGKPAQEKIKMIDKSRHIIITSPHPSPLSASRGFFGSKPFSKTNDALLALGEEPI
                     DWQLPETV"
     gene            3157..4026
                     /locus_tag="IXT20_000920"
     CDS             3157..4026
                     /locus_tag="IXT20_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAQ4859387.1"
                     /translation="MIKLIASDMDGTLLDAHMSVSPENAEAIRFANDAGVEFMVATGR
                     NYQEARAALDEVGIDCAMITLNGAQVFDKEGNSLFTVSIPNNQAISVLDILDANGIYY
                     EVATNDGLYSENQAKRIESFASMVATHLPHLTYKMAIAMASAKLELLHITYVDSIRAI
                     LEEKNLEVLKIICFHTEGPAVLGPVGKQISLLGDLAVTSSGQNNIEVNHKNAQKGIAV
                     AHVAHERGISLEEVMTIGDNFNDVSMLQVAGVSFAMGNAEIEVKDYAKYTTDTNLESG
                     VGKAILRAIAEDL"
     gene            4052..4567
                     /locus_tag="IXT20_000921"
     CDS             4052..4567
                     /locus_tag="IXT20_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4859388.1"
                     /translation="MSEFFIQEKQLGRATRTIVKNEKGHSLFLMVGRWGTKGDALSLY
                     AMNGDLVAHIKQISLTFGTRFELYKEFEKVGTMQKIFNWPGDFYYIRRLHWTVQGDIY
                     NHQYQIHHFNQQIMKMDKATLFSGDYYVLDVSNDEDAPVCICIAAVLDYWLYRKDKSR
                     DKPVSYRLNLS"
     gene            complement(4614..5177)
                     /locus_tag="IXT20_000922"
     CDS             complement(4614..5177)
                     /locus_tag="IXT20_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ4859389.1"
                     /translation="MIRFARKEDAPEIAPLILVILKDMELPFLKQTGEQKTLEILEEA
                     ITDPNYRYSYSRGIVEEIDGKVAGVAYGYTDEEESIIDQPLVPILEKYQLDASTRLFI
                     DKETFPNEWYLDSISVSEDFRGQGIGSRLLEALPKLAKKANRSVIGLSVDEKNPKAKK
                     LYERHDFKVVGQRMISGHLYDHMQKNI"
     gene            complement(5232..5411)
                     /locus_tag="IXT20_000923"
     CDS             complement(5232..5411)
                     /locus_tag="IXT20_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4859390.1"
                     /translation="MEKMKCPNCGKKFAYEEVNNVVEHNDKEMPVVCPYCRTEAARIV
                     THGYFITEKIEDFLK"
     gene            complement(5488..5997)
                     /locus_tag="IXT20_000924"
     CDS             complement(5488..5997)
                     /locus_tag="IXT20_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4859391.1"
                     /translation="MKKMMFGVAALIVIGITGMTGLLNAAFHMDDISDQEYRISLKKA
                     AAIVKNETKEHNISAIHFAVEKDLENPTNATYEYQFFCEGQVIAVNPSTGKTTVRNDT
                     EKAAEHLFDIEKTEEFKQPQAAMKEAVSYYGKKAKVKEWTLLVKDERPYYKVRLIDGE
                     QDRQVWISA"
     gene            complement(6093..6473)
                     /locus_tag="IXT20_000925"
     CDS             complement(6093..6473)
                     /locus_tag="IXT20_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HAQ4859392.1"
                     /translation="MNLTTIHHIAIIVSDYQKSRDFYVNKLGFEVIRENYRKERDDYK
                     LDLKLGSAELEIFGIANAPQRPNYPEACGLRHLAFKVDDIEETIAELQSLGIESEPIR
                     EDTFTGKKMTFFFDPDGLPLELHE"
     gene            6803..7552
                     /locus_tag="IXT20_000926"
     CDS             6803..7552
                     /locus_tag="IXT20_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide transporter"
                     /protein_id="HAQ4859393.1"
                     /translation="MKSYFQFLAHQLKGWPQQNYYLFFFSLGCQVMTLVNSPITWLSV
                     ITFIGTTLGVLCILSINAAKSVNGILGILSAICFIIVGFSAKNYLSIGEQIAYMITLD
                     IPVLLSKEWNHNMASKIRKFTGKSWFIAIISTFLVYLVSGFLIGRYTDDPRPWVDAIA
                     FSISLTAGIISFLRYNNQYYWWLASGLAQMVLWYISFTHGSASLAMFVNSSVYLLNDV
                     LAFTVSPWYNQKERERMQKMEEEYYNAQQHA"
     gene            complement(7736..9013)
                     /locus_tag="IXT20_000927"
     CDS             complement(7736..9013)
                     /locus_tag="IXT20_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4859394.1"
                     /translation="MEEWQSVFEEWFPKEISKSYPIKISKQYTSSQRWEIYAKLTKKQ
                     RELVDKHRRYLISSRFMEEHYLAATDWVFSDFKINPFFRTKRSQQKLYCECGRELKVQ
                     YIVKSPKTGKILKLGINHFADHLHVSPTVAASIHQGMTKVDLALDELLWLKQKNIDFP
                     EGLWQKYCFVLYQNRRMKQPYLPDIKLAQRLAEFRQVEMPIYIADYQALENEIKKISE
                     HINGQSKKRQIKKELFDDFAEELVKDVEEFLINYRAFLRKDWQSIVYEEVPVHPNAYF
                     ETFISVLRKTKRQRTPEVTAQMEYFAKNQRFIQPKIYLFIWKQYCRYGFTEGFFDSIP
                     RIVRNGFLKVLRKEREAIQSADKKDRTVSKEKWQLVVKDIQSGNVQETIDKWKGKHYR
                     FTEAQKQALEYYQKLEESLRFNDEARKYLKELL"
     gene            complement(9092..9736)
                     /gene="pcp"
                     /locus_tag="IXT20_000928"
     CDS             complement(9092..9736)
                     /gene="pcp"
                     /locus_tag="IXT20_000928"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HAQ4859395.1"
                     /translation="MKVLVTGFDPFGGDKVNPAYEAVKKMPDEISGAEIIKVEVPTVF
                     EKSSQVVKEAIQQHQPDVVICVGQAGGRSAVSFERVAINLAEARIPDNEGNQPFDTAL
                     EENGPAAYFTSLPIKAMTKNVQDHGLPAYISYTAGTFVCNDIMYRLLHLIETQFPAIR
                     GGFIHVPFSPDQVIDRPVGTPSMSLEDIASSLTYAVEAAVENKEDISGNAGTTH"
     gene            complement(9935..10879)
                     /locus_tag="IXT20_000929"
     CDS             complement(9935..10879)
                     /locus_tag="IXT20_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HAQ4859396.1"
                     /translation="MDFFTNPDILLGDKLLEILYIVMGLVLIYTGVRNLTDKTNPHRY
                     GSAFFWTVLGVVIAGGRWLPAIVNGGLIFAMTIPAIAKKVSKGESRLPSKEYMEKMSD
                     KLGMKIFIPALSIGVFAILFALFTNLGALVGVGVGVFAAMLILMFFSRDNRPSTFLDD
                     AADMLGTVGPLSMLPMLLASLGAVFTSAGVGTVISNAVGTIVPEGNVNIGIIIYALGM
                     VIFTVIMGNAFAAITVMTVGIGAPFVFSHGANPALIGMVALTCGFCGTLLTPMAANFN
                     IVPVAMLEMKDKYGVIKNQLFIALFMLVFQIAYMILFK"
     gene            complement(10881..11567)
                     /locus_tag="IXT20_000930"
     CDS             complement(10881..11567)
                     /locus_tag="IXT20_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HAQ4859397.1"
                     /translation="MENYLSLLGVLIVILGFAFKLDSILIVMVALVVTALTGGLGIQG
                     MLELLGTSFVNNRGMAIFIIIMLATGTLERNGLKESAASLIKRFKKVSAGMIVDIYGI
                     FRMIFAAFNVSFGGVAGFVRPIILPMALGTIESKGLEVHPKHEEELKGMASAMENVCW
                     FFGQVLFIGGAGALLVQSTLKDLGYDVTLGQLALVEVPVAITALVVASVYFYLKEKKL
                     AKKYYPGKGQ"
     gene            12041..12361
                     /locus_tag="IXT20_000931"
     CDS             12041..12361
                     /locus_tag="IXT20_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HAQ4859398.1"
                     /translation="MAWMELIIAGILEVFWSTMMKWSDGFSKINYSFYTVIGMIASFY
                     FLSKAIKSLPMSLSYPIWTGIGAVGSVIIGVVLFHDKLNLSTWFFVGMLLISIIGIKI
                     TSGH"
     gene            12515..13420
                     /locus_tag="IXT20_000932"
     CDS             12515..13420
                     /locus_tag="IXT20_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ4859399.1"
                     /translation="MKSLSVQELSKNFGHKQALSDVSFSLSPGKIVGLVGPNGAGKTT
                     IMKAILGLIHYSDGTICIGENEISPSSHKGLEQVGALIEYPGIYPFLTGYDHLKLFSE
                     TNDVSAIDTIVNQLKMEKYIHKKAKSYSLGMKQKLGIALALLNHPSIVILDEPMNGLD
                     PQATRDVRKIITDLATQGTSFLISSHLLSELEKMVDELILINQGKIIKQCTMAELNQT
                     AQDFIVISTTNDLAARTILTEAGYTLEDPDKIKLLKENEELLGTVIQLLSAQNIHVTD
                     VQHLTNDLESSLLEILDSVKGEEEK"
     gene            13417..14190
                     /locus_tag="IXT20_000933"
     CDS             13417..14190
                     /locus_tag="IXT20_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAQ4859400.1"
                     /translation="MTLLVKQELFKLIKKKSTAVLSVLLVVLLIGTALLAKKYTTIID
                     PVEMTAQLFSATSWIVFIMIAAASTIISMEAQYGTLKNLLYRKYSRGEILVSKWITLV
                     IYSVYLYLLAIIVTVLMKLILFPSISFTEKVSTGQTLIQSLFTYTLGSYIGLWLILSL
                     VLMIACFINSSGASISAGIVFYFASSIISGILIALIQKWEWIKWNPISMLNLQNQIGN
                     EEIMKQLTHLSTNQMLFGNLAYIVLFLALGYLVFKRKNV"
     gene            complement(14371..15795)
                     /locus_tag="IXT20_000934"
     CDS             complement(14371..15795)
                     /locus_tag="IXT20_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ4859401.1"
                     /translation="MDTEQQKKGIGLIPLAALVIGSAIGGGVFGIMTDISGSAGGPAI
                     FSWVLVGTAMLMLSLSINNINKKRPDLKGGIFSYAEEGFGRFAGFISGWGYWLTCWLG
                     NVAFATLLMSALGYFFPVFEGGQNLASIVMSTVFLWGYAWLVNRGVENASFVNAIVTF
                     CKLVPLFLFIVVAISTFSIQQFIDNFSSDIILNANGQPIPFSSQVLSCIMVMLWVFVG
                     IEGASVVGSRAKKQSDVGKATILGIFGLIIIYVLVSMLPYGTMTMNQLQTIKAQPAMG
                     YVFEMMVGKWGAVVINGGLIISLVGVWLSWTILPIETMGNMAEDGLLPKAWGKLNKNG
                     APTYAIVLTTLCTNVFLLSLLFTDSAYNFAYTLGTAAIFFTWLFLGLYQMKLSYQRKE
                     WVQFLVGLLASAFQVWAMLVVAFNEVMLVLVLFLPGIYFYLKARKEQNEPVQHIHLDK
                     ILLIGVTMVGVIAMVLFATGMLHV"
     gene            16006..16452
                     /gene="nrdI"
                     /locus_tag="IXT20_000935"
     CDS             16006..16452
                     /gene="nrdI"
                     /locus_tag="IXT20_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HAQ4859402.1"
                     /translation="MNILYISISGNTRAFAKHLAEYAEKMHAEDPVNPEVTLKEIHEN
                     SDFTKETEPFFTFVPTYLDGGNGLDNGDTEILTETMREYLEHEDNHKLCLGVVGSGNK
                     NFNNQYCLTAKQYAQTFGFPFLADYELRGTPSDVTRIYQILAENNK"
     gene            complement(16517..18499)
                     /locus_tag="IXT20_000936"
     CDS             complement(16517..18499)
                     /locus_tag="IXT20_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ4859403.1"
                     /translation="MAKNELTLEELAKQIYKGAGGMGNVESIVHCMTRVRMKIINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQQMVDLAGVKLGEKLSSVSAATAN
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAATWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALAAGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKVVPQTSA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVVSSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAAGK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(18513..19409)
                     /gene="murQ"
                     /locus_tag="IXT20_000937"
     CDS             complement(18513..19409)
                     /gene="murQ"
                     /locus_tag="IXT20_000937"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HAQ4859404.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELTSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISKHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEEAQAKLAEANGFVKGTL"
     gene            complement(19412..20473)
                     /locus_tag="IXT20_000938"
     CDS             complement(19412..20473)
                     /locus_tag="IXT20_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HAQ4859405.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQKRLIELGSEAKKNKLELMVDISGEALERAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPKDIDELKEAEADFSQIEAWHNYYPRPETGLDKEWYQKK
                     NQWLKIQGLLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSENVQEQFASFQKEQTVLLHTEPVDEEFYEYILGKHTNRQDDARDVIRSADARF
                     REIPPIPARNTATRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            complement(20684..21211)
                     /locus_tag="IXT20_000939"
     CDS             complement(20684..21211)
                     /locus_tag="IXT20_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HAQ4859406.1"
                     /translation="MLYVTRHGETTWNAQGLVCGRADVPLTEKGQMQAQKLAEKVVDL
                     PVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYDGLPSKDENFQKAR
                     LAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIRVINAYFHPMPNEG
                     FFTFMVDNTELVSYE"
     gene            complement(21278..22780)
                     /gene="lsa"
                     /locus_tag="IXT20_000940"
     CDS             complement(21278..22780)
                     /gene="lsa"
                     /locus_tag="IXT20_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lsa family ABC-F type ribosomal protection
                     protein"
                     /protein_id="HAQ4859407.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKSQPKHLSISYVRQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            complement(23038..24159)
                     /locus_tag="IXT20_000941"
     CDS             complement(23038..24159)
                     /locus_tag="IXT20_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HAQ4859408.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALGGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPHGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            24284..25225
                     /locus_tag="IXT20_000942"
     CDS             24284..25225
                     /locus_tag="IXT20_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HAQ4859409.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFRSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEAKLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKTAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAVWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            complement(25375..26271)
                     /locus_tag="IXT20_000943"
     CDS             complement(25375..26271)
                     /locus_tag="IXT20_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HAQ4859410.1"
                     /translation="MNKNDVLLVAKNKSLYFLPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFNMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMDFSSSEPKTDLFFGATNNGRKERAESYKQEFIKR
                     DVTTNYFILPHRGNEQPGYLAYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYHFYRPENIFLLRERDLDELPEFLHTPFKPIPSELLEFYDAENWAKR
                     FITNDSTIYEQYEYDLSSVENK"
     gene            26540..27880
                     /locus_tag="IXT20_000944"
     CDS             26540..27880
                     /locus_tag="IXT20_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ4859411.1"
                     /translation="MEDKKLKKQLSSRHITMLALGGAIGAGLFKGSGEAIGIAGPSVL
                     IAFLIGGLILYIVMKGLGKIVLSGGDTHHGLSGLIRPYLGAHSADFTDWVYWSMWIIN
                     IIAEAVAAASFLQLWFPHIPAWVFVFMLAVLTTIINLYSVRLFAETEYWLAFAKISVI
                     ILLIIFATYLVGQQMLGTGVFPTLQQLTDHGGFTPHGMKGIISSLLVVIYSYGGSELI
                     AITVSEADDPKRAIPKAIKGVMGRIISFYIIPLFLLLILFPWNTLAGTNVSPFVMVFE
                     KMNIPFAADIVNFVIVLALFSSINSGVYASSRILFFRLKDRKGSSRKLAVLNKHQVPQ
                     RAVLFCTSVLYLGVALSYFVGDKLFGYLAGSLSYTVLLIWIIISAASFVLALRKGTLW
                     DKGISFFALAVLGLIFLGILFTNSIGVTVLTGLLYLFIYFSYQKKNDAFVLTNE"
     gene            28098..28541
                     /locus_tag="IXT20_000945"
     CDS             28098..28541
                     /locus_tag="IXT20_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HAQ4859412.1"
                     /translation="MKKYWKFLLMIGVSTVVMFGMMYLNVYALDHIFFSRTRVFMALM
                     MGAVMAVIMLLFMWKMYENKKINVMILAASLLVFAGSLFMVRSQTTVNDTAWMKAMIP
                     HHSIAILTSKRADLKDPEVKKLAEEIIQAQEEEIDKMKKMIKEME"
     gene            complement(28806..30224)
                     /gene="nhaC"
                     /locus_tag="IXT20_000946"
     CDS             complement(28806..30224)
                     /gene="nhaC"
                     /locus_tag="IXT20_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HAQ4859413.1"
                     /translation="MGKKGTPSFLEATLVLMVIVLGIAIGVVGLKVSPNITILAAIGM
                     VMLYAVIKRYPTDWLHEGIINGIKPGIIPIFIFILVGSLIAVWIQAGIIPTLMVIGFK
                     LISVQWFVPSVFVVCAIVGSAVGSAFTVMSTIGIAFFGIGTTLGLNPALVVGAIVSGA
                     VFGDKMSPLSESTNLAAAIVDADLFKHIKHMMWSTVPAFVVSFFLFMILGHTSKAASL
                     SSIREVTDVLEANFTISIWSLIPLFLMLLCAWKKVPAILTILLNIAVAVGMIFIQNPQ
                     TSLTKLAGVIESGFVSNTGNQQIDSLLSRGGIESMMPTVSLIILTLSLGGLLIEFGLI
                     STVMERVSRKMTNTPKLIFTTLMTSIGVNIFIGEQFLSVILPGNAFKETYEKAGFDPT
                     VLGRTLEDGGTVINYLVPWGIAGSFVASTFGIPTLTYLPFVFFSLLSPVFSMVSAFTG
                     WGISKKFVKPQEAVSKTAQLEQ"
     gene            complement(30627..31850)
                     /locus_tag="IXT20_000947"
     CDS             complement(30627..31850)
                     /locus_tag="IXT20_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HAQ4859414.1"
                     /translation="MTTTISTNQLKDLIQYRLPEYQALALDIHEHPEVSNYEFYSSAA
                     LIKQLEKEGFWVEKEVAGHRTAFDARYISNKPGPTIAFLAEFDALPGIGHACGHNLFG
                     TYSVLAASIVEQFIDETGGEIRVYGTPGEEGGENGSAKGSFVREGFFEDVDVALCVHP
                     AFRYGKTTESLANDPVDIKFYGVASHAAAAPEKGVNALEALIQVFNGINALRLQLPKD
                     VNIHGIITDGGVAANVIPDYAAGRFYLRAGNRQTLDKVYEKVENIVRGAAISTGTTFE
                     FGLFQNAVDDVIVTPSFDDLFFQHAKEAGVPDEEIETEQRTSLGSSDVGNVSQVIPTI
                     QPTVAISETYIAGHTEEFKAAAKSDKGLASIAIAAELLANTALDLLKEPKLLERIKQE
                     HQEIVARKKQEGATF"
     gene            complement(31881..32720)
                     /locus_tag="IXT20_000948"
     CDS             complement(31881..32720)
                     /locus_tag="IXT20_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HAQ4859415.1"
                     /translation="MKKIIRFVVITFVAIIWLAGCSQGKADTTDKKETTVKLGIIGED
                     TDVWDNVKDRLKKENINLEYVKFTDYNQPNAALADGSIDLNSFQHQFFLDNYNKEHGT
                     DLVSIGNTVNAPLGIYSNTLKDVADIKDGAKVAIPNDVTNGGRALLLLQTAGLIKVDK
                     AKGQAPTVSDITENRKNLDISELDASQTARALSDVDISVINSGVAVDAGFNPASDAIF
                     LEPVDDHARPYVNIIVARKEDEKNKTYQKIVDAYQTEDTVKVIEKTSKGSSVPAWETF
                     GRK"
     gene            complement(32737..33417)
                     /locus_tag="IXT20_000949"
     CDS             complement(32737..33417)
                     /locus_tag="IXT20_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ4859416.1"
                     /translation="MTDFFKTYLPNVYLIPDEFVEATKQTLYMSFWTAFIGGMIGIIL
                     GVTLVVTRPNGLLANRLLFEILDKLINIIRSIPFIILLSLLALTTRFLVGTTIGAKAA
                     LVPLIFGVVPFFARQIENALLEVDPGVIEAAEAMGTSPIGIILRVYLLEGLPGIIRVS
                     ALTIINVIGLTTMAGAVGAGGLGNLAISRGYNRFQTDVTIVATLLILILVFLSQFISN
                     QLIKKITH"
     gene            complement(33419..34492)
                     /locus_tag="IXT20_000950"
     CDS             complement(33419..34492)
                     /locus_tag="IXT20_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ4859417.1"
                     /translation="MIELRDVSVCFKQKEKEIQAVDSVDLTIEKGDVYGIVGYSGAGK
                     STLVRVMNLLQKPTAGEVIVNQTNLSQLSPKELRKERKAIGMIFQHFNLMESRTIFDN
                     VDFSLKYSGKSKQERRQKVNELLELVGLEEKASAYPKQLSGGQKQRVAIARALANEPK
                     VLLCDEATSALDPKTTLQILALLKKLNRQLGLTIVLITHEMQVVKEICNKVAVMEDGR
                     IIEKGSSIQIFSNPEEELTKDFIRTATHLDQALETIIAHSAFADQIANKWLVELSYIG
                     NQTNEPLIAHLYSKYQVTANILYGNVELLQETPLGSLIVTLAGETKQRQKALDYLIKS
                     GVKINILQKNELDSRIKVIEGGR"
     gene            complement(35138..35809)
                     /locus_tag="IXT20_000951"
     CDS             complement(35138..35809)
                     /locus_tag="IXT20_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAQ4859418.1"
                     /translation="MKGAIFDLDGLLVDTEKTYRDGWLWGFQQFDIHIPKEIVDAWGG
                     KNWKQSFDILVKAAGSPEKVQEVRAKREEYFYQQLTSGAIHLKPYAKEILADLKERHM
                     KLGIATTTVTKRATDILDHFKLADYFDTFTFGDEVSENKPSPIPYLTALERTGLEASS
                     AFAVEDSLVGATAASRAGLGVVLIPDSSFVRNYTVEEKEKLKLLAEGKDLRTVIEMLD
                     KKTEK"
     gene            complement(35896..38574)
                     /gene="mgtA"
                     /locus_tag="IXT20_000952"
     CDS             complement(35896..38574)
                     /gene="mgtA"
                     /locus_tag="IXT20_000952"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HAQ4859419.1"
                     /translation="MSFGHKGEIEMEKKKDTQKIYAAFAGKTKQELFELFHASANGLT
                     EEQIERSRENYGENAISYGKKTPFIVEILKAYITPFTLVLIALGIISFITEYVLAAPG
                     DKDLFGVAIIFTMVIVSGTMTLIQSVRSNQAAEKLKSLVKVTAAVKRNGKYTEIPMEE
                     IVCGDLVRLSAGDMIPADMRLLSAKDLFISQAALTGESYPVEKHAKVCEDDQQSETSY
                     ENLVFMGSNVISGSAEGIIVSVGNETLFGHVAETLSEKPIQSSFELGIHKTSMLLIKF
                     MSLMAPAVIVINGLTKGDWLEAFLFGLSVAVGLTPEMLPMIVTTNLVKGASVMAKKGT
                     VIKNLNAIQNFGAIDVLCTDKTGTLTQDKIILEYHMDCEGNEDDRVLRHAYLNSYYQT
                     GLKNLLDVAIIDEATETLDTDKINYQKVDEIPFDFERRRMSVVVADPAGKTQMITKGA
                     IEEMLNISKFIDLGGTVTPLTKERKEAVLAKVQSLNEDGLRVIGVAQKTNPSVVGEFS
                     VKDESDMVLIGYLTFLDPPKETTKQALKALKEHGVAVKVLTGDNELVTRSVCRQVGLE
                     INELVTGEKISEMSDHELAQVAEDHEVFVKLNPQQKARLTTALRKNGHTVGFLGDGIN
                     DAPAMKVADIGISVDTAVDIAKESADVILLEKDLTILERGLLSGRETFGNIMKYIKAT
                     ASSNFGNMFSVLIASMFLPFLPMLPLQLLFLNLIYDVSCISLPWDRMDKEYLDEPKKW
                     NASSIGKFMVYFGPTSSIFDITTYLLMYFVICPAVVGGSFHTLDSSQQVIFIALFHAG
                     WFVESLWTQTLVLHALRTPKVPFIQSNVTFAMFVITTLGIIVGSVLPFTSFGAELGLM
                     PLPGNYWLWLFATVIAYLALVTAVKKIYIHRFQELL"
     gene            39233..39670
                     /gene="ndk"
                     /locus_tag="IXT20_000953"
     CDS             39233..39670
                     /gene="ndk"
                     /locus_tag="IXT20_000953"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HAQ4859420.1"
                     /translation="MERTLVIIKPDGVRRHLVGSIIQRFEAKGLAIAEMKFETMTPEL
                     AKEHYQHLTERSFFDELIDYMTSGPVVYLVLVGEEVIDIVRKMVGATKAVDAVPGTIR
                     GDYALPGTENIIHASDSRDAAVKEIARFFPPFDINKPSEKAAK"
     gene            complement(39692..40558)
                     /locus_tag="IXT20_000954"
     CDS             complement(39692..40558)
                     /locus_tag="IXT20_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAQ4859421.1"
                     /translation="MYDGELIKKLRVNKKLTQSQLAEGICSKTSLVGIETNSVKKMSF
                     VTLRSFLERMNMTLAEYEWMRNQLDEPIKLKKKRRMLDRVQEDSFDPYKEISNNRRHF
                     KKTEDLYYLVLSLHMVEKTEVSETEFEFLRYECRIIEEYLMQIPDYGQFELSILADFP
                     WIFSDRFIENNYLKIKKRMRRLAQLNNHEQYLFTFLMNLTSFYIENGRLKKARSVNED
                     MKRSLAHKERSTVIYETLMADYYQRLISAALGEKKTKDYQEFFTVLEYMLGKNQRRSL
                     EKKLLAIAEPSN"
     gene            complement(40718..42811)
                     /locus_tag="IXT20_000955"
     CDS             complement(40718..42811)
                     /locus_tag="IXT20_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HAQ4859422.1"
                     /translation="MTQLKKLCQNRLAIVLTAVFFFWMKTIIAYYADFSLGVEGTIQY
                     FILWINPIATTLLFFGLSLYIKKPKPALAILLIIDILNTLLLYLNIIFYREFTDFITV
                     KSVLGFSKVSQGLSGSSFSLMKPHDVLYWLDIATFIGILVWLTIKKIPIKSQPVSKPM
                     AIAVTCLSGLIFSGNLALSEANRPQLLQRTFDRSYIVKYLGIDAYTIYDGIKTGMTSS
                     VRAHASSNGIDEVLDYTKKHYAEPNPETFGIAKGKNVIVLHLESFQQFLINMKVDGQE
                     VTPFLNSIFQNQATISFDNFFHEVGQGKTSDAENMLETGTFGLPQGSLFTELGSDNVF
                     QAAPAILGQKQGYTSAVFHGNVASFWNRDHVYKNLGYDNFFDRSYFDESDETLGYGIL
                     DKDLFSESAQYLEHLQQPFYTKFLSVTNHTPYYTDDKNFDFPSLNTGNSTVDNYVRTA
                     HYLDQSLEQFFTYLKKSAIYQNSIFVIYGDHFGISNTDNKDLASALGKDPDTWNEFDN
                     AQMQRVPLMIHIPGYTKGSVNHEYGGEIDVLPTLLHLLGIDDKDYLHFGTDLLSPQHD
                     QVVAFRNGNFVTPKYTILGGKAYNNQTGEIIDTKAAGIDEEISKDQEKVKSALSLSDK
                     LNQENLLRFYVPNGFETIDPKKYDYKNEAERNIAIEKQKGEHSTSVFSKNGNKTTTNL
                     YPVTRTDVEQQKDNQ"
BASE COUNT        12458 a         9038 c         7617 g        14052 t
ORIGIN      
        1 tattcaaaaa aaatggactg cgacacgact tttgtcacag tcccttcgtg tgtttttctg
       61 ccttctcaaa aggatgacag tttctcactc ttttcctatt tcttctattc aatcaaataa
      121 ctcattttat atacatctaa aaattcacct agatctgttc ttgcccctct aggcatgacc
      181 gcttcgattt ggaagcccat ttttcggtaa aggtgtacgg ctctttcatt acgaacttga
      241 acgtcaagct ccaatcgaaa aagtacaccc atttccttcg cccaactgat gatttcttct
      301 agcataagag tgcctagtcc cattccccaa tattcctgca gaatactgat accgacttct
      361 cccacgtgag acaaacgaaa ttgactgtct gccttgacag atgctgttcc aataatcgtc
      421 ccttcataga ttgcaagcag caataaatta ttattgcttt cgtatatata atccaactct
      481 tcttctaagg tttctggtgg taacaggagt tctgcttcat ccaaaactaa aaattctgtc
      541 tccttattaa cttgtcccat catcaaaaga atgtcttttg catctcctgg cactgcttcc
      601 cgcaagatga tcccaacttc ttcactcatg attttcagcc catttttcta gaattcgctg
      661 tttgaaggtt tcactttgct ccccgtatga gattagtttc acataccgct tttctaaatc
      721 tgtatcactt ttttctaaaa tcagttccaa ataatcctcc ggcagtgctt cttctaatag
      781 atttccccat tcaacaacgg aaagcccatc accttcaaaa tattcatcta accctaaatc
      841 tgctccgcta gctgctaccc gataaatatc catatgatac aacggaatcc gaccttgact
      901 atattctcta ataatcgtgt atgtcggact tttgatcatt tgcgttatcc ctaatccttg
      961 ggcaattcct ttcgtcaacg ttgttttacc cgcacctaga tcaccagtca atatcagatt
     1021 gtcacctggc attgcggaat gaccaattgc ctctgcgaat tgttctgtca ttgttaatcc
     1081 tgatagttcg atcatttttc atcttctccc gctcttcttt ctttttgcca gtatatcgcg
     1141 taatgaagtt tttttaaaga aaaaatgaac tagtcaagaa cagcagctgc tgtttttggc
     1201 tagttcgcgt gtatttgtta atcaatcaat gtttgtgctg ctgtgataat cgataattta
     1261 tatacatctt cttcatttgc tccacgagac aaatcagaaa ccggtttgtt caagccttgt
     1321 agaattggtc cgattgcttc aaagttaccg aaacgctgag caattttata gccgatattt
     1381 cctgactgca attctggaaa tacaaagact gttgcttgac cagctacttc tgaatctggc
     1441 gctttcagtt gtgcaactga aggaacataa gaagcatcaa attgcaattc gccgtcgatc
     1501 atgtattgtg gcgctagttt tttagcgatt ttagttgctt caactacttt gtctacttct
     1561 ggcgatttcg ctgagccttt tgtcgagaag ctcatcattg ctacttttgg ttcgatatcg
     1621 aacaattctg ctgtttttgc agaatctacg gcgatttcag ccaattcttg cgcagttgga
     1681 ttcacgttga tcgcacaatc cgaaaagaca tatttttctt gatctcttcc acgtaccatg
     1741 atcatcgctc cgcttgtccg actgatgcca ggctttgttt tgatgatctg aagtgctggg
     1801 cgaacagtgt cgccagtcga atggatcgct ccagagacca ttccgtctgc aatccccata
     1861 tacgtaagca tcgtaccaaa gtaattcaca tcttttaaaa tcttttctgc ttgttctttg
     1921 gatgcctttc catttctacg ttcaacaaaa gtatccacca tttcatccca cttatcgtaa
     1981 ttatcagggc tgatgatcgt aaagctagat gttttgattc ctctggcatg cgctgcattt
     2041 cttatttctt cttcgttccc tactaaaatg ggttccacaa gttcttctgc tttcaaccgg
     2101 gcagctgcgc ctaaaatacg agcatcagta gcttccggga aaacgatttt gatcccacgg
     2161 cgaacaattt taaatcttaa actatcaaac aattccacaa gatgacctcc agacgttttt
     2221 atatgtatta tcatacacta ttccagaaaa aaagaacagt ttattttatc tgtatcagtc
     2281 aaaaatgctg gaaaaattaa gtttttcaca tacaaagcga actgcactat aacaggaaag
     2341 cgctgtttta ttacactgtt tcaggcagtt gccaatcaat cggttcttct cctaacgcaa
     2401 ggagcgcatc attcgttttc gagaagggtt tggaaccaaa aaagccacga cttgcagata
     2461 acggactggg atgtggagat gttatgatga tatggcgact cttatcgatc attttgattt
     2521 tttcttgtgc cggttttccc cacaagataa acacgattgg tttttcccgt tcatttaatt
     2581 tttcaatgat cgtgtctgtt aatctttccc aacctttccc gcgatgagaa tacgcttgcc
     2641 cttcccgaac ggtaagaacg gtgttgagta aaaggactcc ctgtttagcc catgacacta
     2701 gatttccatg ttgaacaggt gcgattccaa gatcatcata aagctcttta tagatatttc
     2761 ttaacgatgg cgggatcttt acgccaggtt gcacagaaaa agataagcca tgagcttgat
     2821 tttctccatg ataaggatct tgtcctaaaa tcaccacttt aacttcttca taaggagtca
     2881 attccaatgc ttcatagata tgatacatat ctggatggat cttttgggac gcatattctt
     2941 tttttaaaaa ttctcttaga tgcagataat aatcttttga aaattctgag gacaaaactt
     3001 cttgccaact gttatgaatg atttctttca aaaactccac ctcctaataa aaagaataac
     3061 tcacattttc ttcaatcaca agggacaatc ttcaccctca gcacttgatt aatatggtaa
     3121 actaggaaca agaagcagaa aggaacgata atcattatga tcaaattaat tgcatcagat
     3181 atggacggca cattattaga tgcccatatg agtgtttctc ctgaaaatgc cgaggcgata
     3241 cgttttgcaa atgatgccgg tgttgagttc atggtcgcta ctggtcgaaa ctatcaagaa
     3301 gctcgcgcag ctttagatga ggtaggaatc gattgtgcga tgatcacttt gaacggggca
     3361 caggtatttg ataaagaagg aaattcattg tttaccgtgt cgattccaaa taatcaagca
     3421 atctctgttt tagatattct agatgctaac ggtatttact atgaagtagc aaccaacgat
     3481 ggtctttatt ctgaaaatca agcgaaacga attgaaagtt ttgcttccat ggtggctacc
     3541 catttacctc accttacgta taaaatggcg attgccatgg cctcagctaa gctggagtta
     3601 ttgcacatca catacgtgga cagtattcgt gctattttgg aagaaaagaa tctggaagtt
     3661 ttaaaaatta tttgttttca tacagaaggg ccagctgtat taggtccagt tggaaagcag
     3721 atttctcttt taggcgatct agccgttact tcctctggtc aaaacaatat cgaagtgaac
     3781 cataaaaacg ctcaaaaagg aattgctgtt gcccatgtcg ctcacgagcg tgggatttct
     3841 ttagaagaag tcatgacgat tggcgataac ttcaatgatg tcagcatgct gcaagtagca
     3901 ggagtcagtt tcgcaatggg aaatgcagaa atcgaagtaa aagattatgc caaatataca
     3961 acggacacaa accttgaatc tggcgtagga aaagccatat tgcgcgcgat tgcagaagac
     4021 ttgtagtttt atctagaaaa gagggatata gatgtcagaa tttttcattc aggaaaagca
     4081 gcttggacgt gcaactcgga caattgtcaa aaacgaaaaa ggacattccc tgtttttgat
     4141 ggttggtcgc tggggaacaa agggcgatgc attgtctctt tatgccatga atggcgatct
     4201 cgttgctcac atcaagcaga taagcctgac attcggtacg cgttttgagc tgtataaaga
     4261 gttcgaaaag gtgggaacca tgcagaaaat attcaattgg cccggtgact tttattatat
     4321 tcgtagactt cactggacag tccaaggtga tatctataat catcaatatc aaatccatca
     4381 tttcaatcag caaatcatga aaatggataa agcaacactt ttttcaggag attactatgt
     4441 attggatgtc tcaaatgatg aagatgctcc agtttgtatt tgtattgctg cggttttaga
     4501 ttactggctc taccgtaaag acaaaagtcg cgataagcct gtttcttacc gacttaactt
     4561 aagttaaaaa gatgtacctg aaatgcggaa atacgcattt caggtacatc tttttagata
     4621 ttcttttgca tatgatcata aagatgacca ctgatcatcc gctgaccgac aactttgaag
     4681 tcatggcgtt cgtataattt tttagccttt ggattttttt catccacact tagaccgatg
     4741 acggagcgat tagctttctt cgccaattta ggtaatgctt ctaataatcg cgaaccgatg
     4801 ccttgtcctc taaaatcttc tgatacactg atagagtcca aataccattc gttgggaaac
     4861 gtttctttat caatgaacaa acgtgtagac gcatctagct gatatttctc taagataggt
     4921 accagtggct gatcgatgat tgattcttct tcatctgtat aaccgtaagc aactcctgca
     4981 actttaccgt ctatttcttc tacgatacct cgtgaataac tgtaacgata atttggatca
     5041 gtgattgctt cttctaaaat ctctaaagtc ttttgttccc ctgtctgctt caaaaatggc
     5101 agttccatat cttttagaat cactaagatc aatggggcga tttctggagc atcttctttt
     5161 ctcgcaaaac gaatcatagt ttcctctcct tttctccatt ctaacatgca gaaaaagaaa
     5221 tcgcaaaatc gttattttaa aaaatcttca atcttttcag taatgaaata accatgagta
     5281 acgattcttg ccgcttcagt tcggcaataa gggcagacga ctggcatttc tttatcatta
     5341 tgttcgacta cattattgac ttcttcgtat gcaaattttt ttccgcaatt agggcatttc
     5401 attttttcca tgttatctca acttcctttc atctttactc tcttagctta ctcttatatt
     5461 gtaaaaaaga gaagcaatat ccttcttcta tgcagatatc catacctgac ggtcctgttc
     5521 tccatctatt aatcggactt tataataagg acgttcatct ttgactagaa gtgtccactc
     5581 tttcactttt gcttttttcc cgtagtagct gactgcttcc ttcatcgctg cttgcggttg
     5641 tttgaactct tccgtttttt ctatatcaaa aagatgttct gcggcctttt ccgtgtcatt
     5701 tcgtacggtt gttttcccag tggatggatt gactgctatg acttgtcctt cacaaaaaaa
     5761 ctggtattca tatgttgcat tcgtaggatt ctccaaatct ttttcaactg caaaatgaat
     5821 agcagatatg ttatgttctt ttgtttcgtt ttttacgatt gctgctgctt ttttcaatga
     5881 gatccgatat tcctggtcac taatatcatc catgtgaaaa gcggcattga gcagtcccgt
     5941 cataccggta atccctatta cgatgagggc agcaacacca aacatcattt ttttcattct
     6001 tcattctcct gtttcttttt tttaaagaat atcaagaaat gaacgaatga aaaatcgaat
     6061 gaaacgaaaa aaaagtgaca attttgttat ctttattcgt gcagttctaa tggcaatccg
     6121 tctggatcaa agaaaaacgt catttttttt cctgtaaagg tatcttctct aatcggttct
     6181 gattcgatcc ctaaggactg caattctgca attgtttctt caatatcgtc tactttgaaa
     6241 gcaagatgac gcaagccaca agcttctgga tagtttggtc gttgtggcgc attagcaatt
     6301 ccaaaaatct ccaattcagc acttcctagt ttcagatcta atttatagtc atctcgttct
     6361 ttgcggtaat tttctctgat tacttcaaat cctaatttat taacataaaa atcacgtgat
     6421 ttctgataat ctgaaacaat gatggctata tgatggatcg ttgtaaggtt catatgagca
     6481 ctcctattct tcgtttaagc tcggatctat attaatctcc agttccattg taaatgtgtc
     6541 agattgaaaa aaatagctgt ttttcctttt tataagaaaa aaggcaaaaa aaacagaaag
     6601 taggtcctcc aagacactct tcctgttccc aatataataa atgaaagtaa gaaattatat
     6661 tttcaagaaa acaaacagaa aattatcata acacaaatta tttataattg aaagactagt
     6721 tttttttcaa gaaaagcata caatagattg tgttagtaaa tacagaacac actacattta
     6781 gtgtcttaag gagtcctcat acatgaagtc atattttcag tttttagctc atcagctaaa
     6841 aggctggccg caacaaaatt attatctttt ctttttcagt cttggctgcc aggtcatgac
     6901 tcttgtcaat agtcccatca cttggctgtc cgttattaca tttatcggta ccacgcttgg
     6961 tgtattgtgt attttatcca tcaatgcggc aaaatcagtc aacggtattt taggtatttt
     7021 gtctgcgatt tgttttatta tcgttgggtt ttcagcaaag aactatttaa gtatcggtga
     7081 acagattgct tatatgatca cattggatat ccccgtattg ttaagtaaag aatggaatca
     7141 taatatggct tcaaaaatcc gaaaattcac tggaaaaagc tggtttatcg caatcatcag
     7201 tactttcctt gtctatcttg tttcaggatt cctgattggt cgttatacag atgatcctcg
     7261 gccgtgggta gatgccattg ctttttctat tagcttgact gctggtatca tcagtttctt
     7321 gcgttacaac aaccaatatt actggtggtt agcttcaggg cttgctcaaa tggtcctttg
     7381 gtatatttca tttacgcatg gatctgcaag tttggcgatg ttcgtgaaca gctctgttta
     7441 cttgctgaat gatgtccttg catttaccgt ttcaccttgg tataaccaaa aagaacgtga
     7501 acgtatgcaa aaaatggaag aagaatacta caacgcacaa caacacgcat aaaaaaactg
     7561 agcgtgggac aaaagtaaag aacacttttg tctcacgctc taaacacgta taaacggcgg
     7621 aatcagaagc aactccttcg gaaataagct gaaattcaca aaaatttgaa aagcaatttt
     7681 cgtgaatttc ttcttatttc ttggagttaa acgcttctgt cccagcctct attttttata
     7741 gtaattcctt cagatacttt cttgcctcat cattgaaacg cagtgattct tctaacttct
     7801 gataatattc caacgcttgt ttctgagctt cagtaaaacg ataatgtttt cctttccatt
     7861 tgtcgattgt ctcttgcaca tttccagatt ggatatcttt cactactagt tgccattttt
     7921 cttttgagac tgtacggtct ttcttatcag cagattgtat agcttctcgt tctttacgca
     7981 atacttttaa aaaaccattt ctgactatcc gaggaatgct gtcgaagaac ccttctgtaa
     8041 aaccatagcg acaatattgc ttccaaataa atagatagat ttttggttgg atgaatcgtt
     8101 ggtttttcgc aaaatattcc atctgagcag tgacttctgg tgttcgttga cgttttgtct
     8161 ttctcaacac ggaaataaat gtttcaaaat aagcattagg atgtactggc acttcttcat
     8221 atacgatgga ttgccaatct ttacgtaaaa atgcgcgata gttgatcaag aattcttcta
     8281 catcctttac taattcttct gcaaagtcgt caaatagttc tttttttatc tgtcgctttt
     8341 ttgactgccc attgatgtgt tcacttattt tcttgatttc attttccaat gcttgataat
     8401 ccgctatgta aatcggcatt tctacttggc gaaattctgc gagccgctga gctaatttta
     8461 tatccggtaa atatggctgt ttcatccgac gattttggta caagacaaaa cagtattttt
     8521 gccatagtcc ttcaggaaaa tcgatattct tctgcttaag ccataataat tcgtctaagg
     8581 ccagatcaac ttttgtcatt ccctgatgga tcgaagctgc cactgtcgga gagacatgca
     8641 ggtgatcggc aaagtggttg atgcctagtt ttaggatctt tccggttttt ggcgatttaa
     8701 cgatgtattg cacttttaat tctctaccac actcacaata caatttttgc tggcttcgtt
     8761 ttgttcgaaa aaacggattg atcttgaagt cagaaaacac ccaatctgtt gcagctaaat
     8821 aatgttcttc cataaaacga gagctgatta gatatcggcg gtgcttatcg actagttccc
     8881 gctgtttttt cgttaatttt gcatagatct cccaacgttg actgcttgtg tattgtttag
     8941 aaattttgat gggatagctt ttgctgattt cttttggaaa ccattcttca aagacggatt
     9001 gccattcttc cacggctgtt tctttccttt ctaattacaa aaagctggct actaacataa
     9061 cgttagcaac cagccagtgt ttcttgctaa attagtgagt tgttccggca tttccggaaa
     9121 tatcttcttt attttctact gctgcttcaa ctgcataagt caaagaagaa gcaatgtctt
     9181 ctaaagacat agaaggcgta cccactggac gatcgatcac ttgatcgggt gagaatggca
     9241 cgtggatgaa cccacctcga attgccggga attgtgtttc gatcaaatga agcaaacgat
     9301 acatgatatc attgcagaca aatgttcctg ctgtataaga gatgtacgct ggtagaccat
     9361 gatcttgtac attttttgtc attgctttga ttggcaatga ggtaaagtaa gcggctggac
     9421 cgttttcttc taaagccgta tcgaatggct gatttccttc attatcaggg attcttgctt
     9481 ctgctagatt gattgcgaca cgttcgaacg agactgctga ccgcccacct gcttgtccga
     9541 cacagatcac gacatctggt tggtgctgtt ggatggcttc tttgactact tgactgcttt
     9601 tttcgaatac ggtcggtact tccactttga tgatttcagc accgctgatt tcatctggca
     9661 ttttttttac tgcttcatag gctggattca ctttgtcccc accaaatgga tcaaaacctg
     9721 ttactagtac tttcatctgt tgttcctccg tccgattgat ttaacatatg gttatctttt
     9781 atcataccat tttccttgaa cagcgtaaat aaaggaaaca atagtttaaa taacataaca
     9841 gatcaaactt aatatattct tataaaaaaa tagtgttcca ctacaatgtt agaagaggtt
     9901 gcgacaatta ttctgtcaca acccctctct tctattattt aaatagaatc atataagcaa
     9961 tttggaaaac tagcataaat aacgcaataa acaactggtt tttgatcact ccgtatttgt
    10021 ctttcatctc cagcatcgct acaggcacga tattgaagtt agcagccatc ggtgtcagca
    10081 gggtcccaca aaaaccgcaa gtcaatgcaa ccattcctat taacgctgga ttcgctccat
    10141 gactaaatac gaatggtgca ccgatcccaa ccgtcatcac ggtgatggcc gcaaatgcat
    10201 tccccatgat taccgtaaat atcaccatgc caagagcgta aatgatgatg ccgatgttca
    10261 catttccttc tggaacgatc gtacctactg cattggaaat cactgtccca actccagcgc
    10321 ttgtaaatac cgcacctaga gaagctaaaa gcattggcaa catgcttaac ggccctactg
    10381 tgcccagcat atctgctgca tcatctaaaa atgtacttgg tcgattatct cgagaaaaga
    10441 acatcaagat tagcatggct gcaaaaacac cgacgcccac gccgactaaa gcacctaaat
    10501 tggtaaatag agcaaacaag atcgcaaaga caccgatact caaagctgga ataaaaattt
    10561 tcattcctag tttgtctgac attttttcca tatattcttt tgatggaagc cggctttctc
    10621 ctttgctgac tttcttagca attgctggta tggtcatcgc aaagatcaag ccaccgttca
    10681 cgattgccgg aagccatctt cctcctgcaa taactacccc taagaccgtc cagaaaaagg
    10741 ctgacccata acgatgagga ttggttttat ctgttaaatt tcttactcca gtgtaaatca
    10801 atacgagtcc catcacgata taaaggatct ccagtaattt atcgcctaat aaaatatcag
    10861 gatttgtaaa aaagtccatc ttattgcccc tttcctggat aatatttctt cgctaatttc
    10921 ttttctttaa gatagaagta gacacttgcc acaactaaag ccgtgatagc cactggaact
    10981 tccactaaag caagctgtcc caatgttacg tcatatccta gatctttcag tgttgattgg
    11041 actagcaacg cccctgctcc tccgatgaat agtacttggc caaagaacca gcagacattt
    11101 tccatagctg aagccatacc ttttagttct tcttcatgct taggatggac ttctaaccct
    11161 tttgattcga tggtccccaa tgccatcggt aaaatgattg gacgaacaaa tccagcaact
    11221 ccaccaaaac tcacgttaaa agccgcaaag atcattcgaa agatcccata aatatctaca
    11281 atcataccag cagatacttt cttgaatcgc ttgatcaagg aagccgctga ttctttcaga
    11341 ccatttcttt caagcgttcc tgttgccaac ataatgatga taaaaatcgc catcccgcga
    11401 ttgtttacaa aacttgtacc taaaagttct aacattcctt gaatgccaag accgccagtc
    11461 aaagctgtaa caactaaagc aaccattacg atcaatatcg agtctaattt aaatgcaaac
    11521 ccgagaataa caatcaagac gcctaaaagg ctaagataat tttccatata ttttcctcca
    11581 taaaatgaca atattttttt aattataaag caaaacacat aaaaaatata gttgttttct
    11641 cacgaaatag acactatttt taaatatttt tcgttatttt tttatcacaa aaattatctt
    11701 acttattgta aggtaaagct ttttttaatt tattgcccga taatatatag aaataaggtc
    11761 tagttactgt tttttttaaa tgattaataa ataacaactt ctttctatga aattgcaaag
    11821 cattcatctt cttttccgct aacgatatat ccgtcagtgc ttggtacact ttactaaaaa
    11881 gtatgagatt gccgatatct ggataggtgt gctatactac agaaaaagat atacgatctg
    11941 ccaccgggta ggataaagag gatgatcatt ccattaggtc ttttaaggga tgattttcct
    12001 ctttttgtgt atttttttat agttaaggag gaatgtaagt atggcatgga tggaattaat
    12061 catagcagga attttggaag tattctggtc aacgatgatg aaatggagtg atggtttttc
    12121 taaaatcaat tattcttttt acacagtgat tggcatgatt gctagttttt actttttgtc
    12181 aaaggcaatc aaaagtttgc cgatgagtct ctcttatcct atctggacag ggattggcgc
    12241 agttggttcg gtcataattg gtgtggtctt gtttcatgat aagctgaatc tttccacttg
    12301 gttctttgtg gggatgctac tcatcagtat cattggcatc aagattacta gcggccacta
    12361 gacacttgga ataagtactg atcagaaaaa ataagccttt ttatttaata ttcaattttt
    12421 ttatgaaaaa ataagaaata agtccaatga cctagcgact tctccactca tcaggtgtat
    12481 gcttgtacta tctatagaaa aggagtgaat atttttgaaa agtctttcgg tccaagaatt
    12541 atccaagaac tttggtcaca aacaagccct cagcgatgtc agttttagct tatcccctgg
    12601 caaaatcgtt ggattagtag gtcctaatgg cgctggaaaa acaacgatca tgaaagctat
    12661 tttaggactg atccattact cagatggaac aatctgtatt ggtgaaaatg agatttctcc
    12721 ctctagtcat aaagggttgg aacaagtagg agcattgatc gaatacccgg gtatctatcc
    12781 attcttaaca ggctatgatc atctgaagtt attctctgaa accaacgatg tatctgctat
    12841 cgacactatc gtgaatcagc tcaagatgga aaagtacatt cacaaaaaag cgaaatccta
    12901 ctcgttggga atgaaacaaa aattgggaat tgctcttgcc ttactgaacc atccgtctat
    12961 cgttatttta gatgagccga tgaacggttt ggatccacaa gcaacaagag atgtgcgaaa
    13021 aatcattacc gacttggcta ctcagggcac ctctttcttg atctccagtc acttactcag
    13081 cgagcttgaa aaaatggtcg atgagctgat cctgatcaat caaggaaaga tcatcaaaca
    13141 atgtacaatg gctgaattga accaaacagc gcaagatttc atcgttatct ctacaacaaa
    13201 tgatctagct gctcgaacga tcttgacaga agctggatat actttggaag atcctgataa
    13261 aatcaagctt ttaaaagaaa acgaagaatt attaggaacc gtgatccagc tattgtctgc
    13321 acaaaatatc catgtgacag acgtccagca tctgacgaat gatttggaat cttctctatt
    13381 ggaaattctg gattcagtaa agggggaaga agaaaaatga cattgcttgt aaaacaagaa
    13441 ttatttaaat tgatcaagaa aaagtcaact gctgttttat ctgttctatt agttgttttg
    13501 ctcataggaa cagcacttct agccaaaaaa tacacgacga tcattgatcc agttgaaatg
    13561 actgctcagc tttttagtgc cacatcttgg attgtcttta tcatgattgc agctgcaagc
    13621 acgatcattt cgatggaagc acaatatggt acattgaaaa atctccttta tcgaaagtat
    13681 tctcgaggag aaatattagt aagcaaatgg atcacattag ttatttattc tgtttattta
    13741 taccttttag ctattattgt cactgtgttg atgaaactga ttcttttccc ttcgatttcc
    13801 ttcacggaga aggtttctac tggtcaaaca ctgatccaaa gcttgttcac ttatactttg
    13861 ggtagttata tcggtttatg gctgatttta agtttagtgt tgatgattgc ttgctttatc
    13921 aatagttccg gtgcatccat ctcagcagga atcgtctttt attttgcttc gtcaatcata
    13981 tctggtatcc ttattgcact catccaaaaa tgggagtgga tcaaatggaa tccgatcagt
    14041 atgttgaatc tgcaaaacca aattggaaac gaagagatca tgaagcaatt gactcatctt
    14101 tctactaatc aaatgctatt cggtaattta gcttacatcg ttttgttctt agcattagga
    14161 tatctcgtat ttaagcgaaa aaatgtatga tgactcatca aaaaagaggc tgtgaaagag
    14221 acgttcagcc ttattgccga ttggctgtct cttaaacacc gtttattctg aaaaaggagc
    14281 tgtgacaaca tttgtgtcac agctcctttc ctagtatgtt tttgaataaa cgacaggctc
    14341 acaacctctc tttatacttc ttttatactt ctatacgtga agcatcccag tggcaaataa
    14401 aaccattgca atcactccga ccatcgtcac acctataagt aaaatcttat ctaagtggat
    14461 atgttgtaca ggctcgtttt gctccttacg tgctttgagg taaaaataaa tgcctggcaa
    14521 gaaaagaaca agaacaagca ttacttcatt aaaggctacg actagcatag cccatacttg
    14581 aaaggcactt gctaaaagcc ctactaaaaa ttggacccat tctttccgct gataagaaag
    14641 tttcatttgg tacaatccta agaaaagcca cgtgaagaaa atagctgctg taccaagtgt
    14701 gtaagcaaag ttataagcac tgtcagtgaa taataatgaa agtaaaaaga cattcgtaca
    14761 taacgtcgtc aatacgatag cataagttgg tgccccattt ttgttgagtt ttccccatgc
    14821 tttaggcaaa agtccgtctt cagccatgtt tcccattgtt tcaatcggta agatcgtcca
    14881 tgagagccaa acaccaacta aagaaatgat caaaccgccg ttgatcacga ctgctcccca
    14941 tttaccaacc atcatttcaa acacatagcc catagcaggc tgcgccttga tcgtttgcag
    15001 ctgattcatc gtcattgtcc catatggaag catggaaacc aagacgtaaa taataatcaa
    15061 gccgaatatc cctagaatcg ttgctttccc aacatctgat tgtttttttg ctcgtgaacc
    15121 tacgacagag gctccttcaa taccaacaaa tacccaaagc atcaccatga tgcaactcaa
    15181 tacttgactg gaaaacggga tcggctgtcc attcgcattc aatataatgt cgctagaaaa
    15241 gttatcaatg aattgttgga tcgaaaacgt gctgatagct actacaataa ataaaaataa
    15301 tggcactaat ttacagaatg tgacgatagc attgacgaaa ctagcatttt cgactccccg
    15361 attcactagc catgcatatc cccataaaaa gactgtactc atcacgatcg atgccagatt
    15421 ttgcccacct tcaaatactg ggaagaaata tcctaatgcg ctcatcaaca atgttgcaaa
    15481 agctacgttg cctaaccagc atgtgagcca gtaaccccaa ccgctgataa aaccagcgaa
    15541 acggccaaaa ccttcttctg cgtaactaaa gatcccgcct tttaaatctg gacgtttttt
    15601 attgatatta ttgatcgaca aggagagcat caacattgct gttcctacga gtacccaact
    15661 gaaaatagct gggccgccag ccgatccgga gatgtccgtc ataataccaa atacgccacc
    15721 tccaatcgct gaaccaatta ctaaggcagc aagtggaatt aaaccaattc cttttttctg
    15781 ttgctctgta tccatgtaag catcctccaa aataaaaccg cttttatcaa aaagtataac
    15841 aaaccttttt tatttttact taattaataa aaactttaat agaaatataa ttctaccggc
    15901 ttttttctca ttatacaagc aaagcgccta gtccctttcc aaattacacc accaaaaaac
    15961 ttaaaaaaga tgctatacta aaaggaaaga attggaggat gatccatgaa cattttatat
    16021 atatcgattt caggaaatac gcgtgccttt gccaaacatc tagcagaata tgctgaaaaa
    16081 atgcatgcag aagatcctgt caatcctgaa gttactttaa aagaaatcca tgaaaatagt
    16141 gattttacga aagaaacaga accatttttt actttcgttc ctacttattt agacggcgga
    16201 aatggtttag ataacggtga tacagaaatc ttgaccgaaa cgatgcgtga atatctggaa
    16261 catgaagaca atcataaact ttgtcttggt gtagtcggta gcggaaataa aaacttcaat
    16321 aatcagtatt gtctaacagc caaacaatat gcacaaacat ttggctttcc tttcttggca
    16381 gattatgaat tacggggaac accatctgat gtgacacgaa tttatcagat tttagcagaa
    16441 aacaataaat aataaaaaag aagaagctga aaaatagatt tcagtttctt ctttcgtttc
    16501 tattgtttct atcatattaa gctttgacat ccatcacagg tgcttttgct tttgctttac
    16561 ctgttttttc caagacaaag ttttttacac gatccatatt ggttaccaag acgatcatgg
    16621 atgtttcttt gcctgctgct ttgatcgctt ctaagtcagc ttctgctaca agcgtatcag
    16681 gtgtcacttg ctgcccttct gttactttca catcaaatgg cgtaccgcct aagtcaactg
    16741 tgttgattcc catatgaagc aagatttcta aaccatttgg cgttgtgatc ccaatagcgt
    16801 gtttcgttgg gaagacagag ctgactaccc cagtaactgg agaatagata tgtccgtttt
    16861 ctggttctac aaagtagcct tcacccatca tcttttgtgc aaaaactgga tcacttgctt
    16921 tatctaaaga aacaactgtt ccgtcagcaa ctgatgagag gatagtcaca ctttcagctg
    16981 aagtttgagg tactactttt tcaggcactt catttactgt ggctgtttca gtcgctaatt
    17041 cttctttttc taaacggtct ttaggaatac cgaagaagaa tgtagcaacg aagccaccag
    17101 cataagctgc caataatcca aggatgtagc ctaaccattt accgtcagag atcaatggga
    17161 tcaaagcggc tcctgaaggt ccaatagcga tagccccgac atttccgatc attccgatca
    17221 ctgccccgcc gatacctcca ccgatacaag cagtaataaa tgggcgtcct agaggtaatg
    17281 ttactccgta aatcaatggt tcgccgattc ctaggatccc gactggtaaa gctcctttga
    17341 tcatttctgt taattcttta tcttttttgc agcgaatcca taatgccaat gctgcaccca
    17401 cttgtcctgc accagccatc gcaagaattg gaagcaatag cgtacgacca gtttcatcaa
    17461 tcatttgcac atggattgga gtcaaaattt gatgcagtcc gaacataacc atcggcaaga
    17521 ataatgtgcc tagaacgaag ccggcaaatg ctccaccaac gccaagtacc cagttgatcc
    17581 caccgaccat gccatcagaa atgaaacctg ctaaaggcat gatgaagaaa atttcaatca
    17641 gaccgatggc aatcaatgaa agagttggtg taacgatgat atctaccgca tcaggtacta
    17701 ctttatgcag ttttttctca acgattgaca atagccaaac agcgaagatg acacctagga
    17761 tcccaccttg tccagctgca agagcttcac cagtaaataa gtttttcagt ggtgcttccg
    17821 gattcatccc tgtcagcatc acaacggccc cgataactcc accaagagtt ggtgttgcac
    17881 caaaaacttg tgcagcattg atacctgtat agataactag gtagctaaac atcccgtttt
    17941 tcaagatgtt catcacatcg atatattgtt gccatgttgc tgcatcaagt gttccggctg
    18001 tgactaagtt tgctaaaact gcggcaatcc ctgccacgat cccagtaccg acaaatgcag
    18061 gaatcattgg cacaaagata ttagaaatgt cttttaatac cgatttgatt ttagatggtt
    18121 tttgtttcga tttttgtgcc gctttcattt cttgtgcccg ttcgtttaca cggtcttttc
    18181 cagatgctga tgaatttgct gtagctgcac ttacagaaga aagtttttct cctaatttca
    18241 cgcctgccaa atcaaccatt tgctgtgcta ctttgttcac tttcccaggt ccgatgatga
    18301 cttgaagctg ttcatcttct actacaccaa gcactcctgg aatcgctttt aattcagaga
    18361 catttacaag atcatcattg atgatcttca tacgcacacg agtcatgcag tggacaatgc
    18421 tttctacatt gcccatgccg cctgcccctt tataaatttg ttttgccagt tcttccaaag
    18481 tcagttcgtt ttttgccatg gtcttttccc cctcataagg tcccttttac aaatccgttt
    18541 gcctcagcta attttgcttg agcttcctct ttatcactat ttgtcaaaat catgacgata
    18601 gccagcttaa cattttgatc tgcttcttca aagtactttt cagctgtttc gtaatcagct
    18661 tctgtcgctt gcatgatgat tcgttttgag cgttcgatta gtttttcatt tgttggtttg
    18721 acatcgacca tcaagttatt gaatacttta cctataccaa tcatactgat agtagaaatc
    18781 atattcaaga taagtttttg tgctgttcct gacttcaatc gagtagagcc tgtcaaaaat
    18841 tctggacctg catcgacttc gattgggaaa tccgcgtgtt tactgatttc ggcttgttta
    18901 ttgcaagaaa tcgttcctgt gacagcaccg atcgattttg cgtaatctaa tccaccgatc
    18961 acatatggtg tacgaccgct agctgcgata ccaataacta tatcattttt tgtcaaattc
    19021 agcttgatta gatcttcttg ccctagctca taagaatctt cggccccttc gacagctaca
    19081 gtcattgctt cttctccacc agcaattaga cctatgacca tttccggttc tacaccgaat
    19141 gtcggtacgc attcagcagc atctaacaca cctagtcttc cacttgttcc ggctcccata
    19201 taaatcagac gtccaccttt tttaaaggac tcaatcgtgt tagcaatcac tggttcgata
    19261 cttgtcagtt ctttttcaac cgcagcggcc actttctgat cttctttgtt cattagttga
    19321 acggcttgag aaatactcat ctcatctaat ccaaaagttt cttggtttct gcgttccgtt
    19381 gttaggttct ctaaattgat tttgctcaat tttatcttcc ttcttttctt ataaattgat
    19441 agttcatacc agctttgatt tggttgatca atggtagctc ttctttgata actttagcta
    19501 ctacattcac tttttcatcc gctggcaaat catatttggt taattggatc tcacccatat
    19561 agcgcaaata cttttcgtta tctaaagtaa tagacccttt catacgcgta gctgtatttc
    19621 ttgctgggat aggcggaatc tctcgaaaac gtgcatctgc gcttcgtatg acatctcttg
    19681 catcgtcttg ccgattcgta tgcttcccta atatatattc gtaaaattct tcatctacag
    19741 gttccgtatg gagcaagact gtctgttctt tttggaaaga agcaaattgc tcctgaacgt
    19801 tttctgacag acctgcatct ccgatgtaaa caatatccgt gttacttgct aatagatcta
    19861 gagcgcctgc aagaggatgg atccctcgat gttcttccaa agttggcaat ccttgatata
    19921 gtgggcctct caactctgta tctcctggta caaaaccttg gacaaggaga ccttgaattt
    19981 tgagccattg attttttttc tgataccatt ctttgtccag ccctgtttcg ggacgtggat
    20041 aataattatg ccaagcttca atctgtgaga aatcagcttc tgcttctttc aattcatcga
    20101 tatctttagg cgtgatcgtg ctagcattca aactgatttt caattgatgt gaccagtcgg
    20161 caatctgctg attggaaata tgataatcca ttcttaaacc tgttacacca attgctttca
    20221 aaggcgttag atcttctaat gaaaaacccg ctcgttccaa tgcctctcct gaaatatcga
    20281 ccatcaattc tagtttattc tttttcgctt cgcttcctag ttcgatcaat cgcttttgat
    20341 atgccgaaac atcatcttct ggtatatgca tggaagtgaa aataccgaca aagccattgg
    20401 atgccatttg gcggatatac tgtttcttct cttctgacaa agaatcgttc ataaatattg
    20461 aaaagccaaa catattatcc ctctttttct gtatccgtct tcatttctat gactagagta
    20521 tatatctaaa gaaacaaaat ttcaatactt ttattaaaat aattaaattt catttctaaa
    20581 ggttgagttt atattatcaa aagaattgtc ataacctaaa aaaggcactg ctaagcaaaa
    20641 gcagtgcctt gaaatatttt atttaaataa cacaaaaaca taattactca taagaaacca
    20701 attctgtatt atctaccatg aatgtaaaga acccctcatt aggcatcgga tgaaaataag
    20761 cattgatcac tcttatcaag gcattatggc aaactaacag atagacattt tcttcgtctt
    20821 cgatgcattc tttcaataaa ggaacgatcc gtgcatacac atctaagact gactccccat
    20881 ttgggaatcg tacagcgaac gctaaacgtg ctttttgaaa gttctcatct ttgcttggca
    20941 gtccatcata atccccaaaa tccatttcta ccaatcgttc atcaacggtc agcggcaagc
    21001 tgagacgatc agcaactgct tgtgctgtat ctcttgctcg ttgaagaggg gaatggatga
    21061 ttttcgtgat tggaacaggc aaatctacta ccttttctgc tagtttctgt gcctgcatct
    21121 gtcctttttc agtcagcggc acatctgccc gtccgcaaac taatccttgt gcgttccaag
    21181 ttgtttctcc gtgtctcgtt acataaagca tgtgattctc tccttcgttg gcaaagttcc
    21241 ccttaaccat accttgttgt tcttgagatt acaagggtta gattctctca agagaaataa
    21301 tctcagtaga tactttatcc aggaaggttt gatcatgttc cactagtaac atggcaggtt
    21361 tcacgttcaa gatcagttgt tctaattgtt cttgattgaa gacatccaaa taattcaatg
    21421 gttcatccca tgtatatagt tcagctggct gtgataaaga tttagccaat tccacttttt
    21481 tccgttggcc catactcatc tgctcgatct tgttatgaaa aacatctctt tccatcccaa
    21541 gctttcggag gttgttcaaa aatgcttggt agtctacttg gtttttctct gcaaattccg
    21601 ccaacgttcc tcgattgtct tcataatttt ggcgtacata actaatgctc agatgttttg
    21661 gctgggattt ttctcctata accttgccgt tgaatgcccc cagaagataa tggataatgg
    21721 atgactttcc tgcaccgtta ggacctgaaa tcgccacccg ctgatgaggc tcgattgtaa
    21781 aatgaattgg ctcgaataac agattttcat accctaattg aagatcttct acgcttaaaa
    21841 gtcgcttatg gtgagacgct tggctattca tcgtcaaaga atcgatatat tcgatatctt
    21901 ttaatagttt ttctttctcg ctgatctggg tctccatccg attcacgatt gctttggatc
    21961 gtttcatcgt ccgtgcagca tcagcaccca ctgctccttt attcactcgt ctagattcag
    22021 tatcgatgaa tccgacttgt ttctttgttt tatctccttc tcgggaacga gaccattcgg
    22081 ctttttcagc tgctgttttc tttagcctac tgacttcttt tttcaatttt tcattttgag
    22141 ccatttcaaa ttcatcacga agttttttct gttcttcata gatagagaaa ttcccttgat
    22201 aaagttccag ttgacttttt tcgattgcta aaacatggtc cactacttca tcgataaatc
    22261 cccggtcatg gctgaccacg atgaagcctt gttttttctt tttcaaataa gccgctactt
    22321 gttttctacc agagatatcc aaatgattcg ttggttcatc gattaacggg aaatgagtgt
    22381 catccacaaa taaaagtgcc agtaggactt ttgtcttctc tccccccgat agtgtgctga
    22441 attctctcca taagatttca ggatctgttt gcatcaattg gagctctctt tcgatttccc
    22501 atatctcaaa atccgtaata tcatttaaca cgtaataggt taaacgttct ttatcttttg
    22561 tctgttgcgg gaaataggca aattcttgct gatggattac ttgcccttga taaggtagtt
    22621 tgttttgtag aatattcagt aaagttgtct ttcctcgacc gtttcgtccg ataagtccta
    22681 gtttccattg tgtgtcaaaa tttagatttg cttgttcaaa taataatgtg ccttggctgt
    22741 cgtagccgaa tgtcagattt tttatttcga ttttagacat tttccatcac ctcgtaaaaa
    22801 aagaccagtc ctattttcag aactggctaa agaggatgca agggttggat acacacaaaa
    22861 agacccatac atcgaaaaaa ccaagccaat cccgaaaatc tgcacactgc ttccttgtct
    22921 ccacaaggta cagtctgaat gcagctttca tcaatcgact taatttttca atgcacggat
    22981 ccctcacttt gtcctatatt tgaaattacg atagcataaa gaaaatgatt ttgtcaacta
    23041 cttcgggagg atggtgaaaa atctgctaag tgtctgtcct aagatttttg ttttttctaa
    23101 tgtttgcctg tgttccattg cttcttctaa acctattgga cccaaatgaa tacttaagac
    23161 aattgggaga aaatcagaga gtccttctag ctcttggtct ctacttccgc aaatagcgat
    23221 tattggtttt cgatattttt ttgcaagcac tgctacaccg tgtggaactt ttccgtggat
    23281 cgtttgctga tcgatccgcc cttctcctgt aatgatgaca tctgcttgtt cgatcttttt
    23341 ttccaatcca gtgatttggc tgactaaatc gaaaccagaa accatcttcg ctcccaacaa
    23401 aaaaagggct ccaccaatac cgcctgctgc tccacttcct ggaatatcat ctaaatccac
    23461 ctcccagaaa tcttttattt gctgagcaat ctttcctgct tgtttatcta agaatgctag
    23521 cgtcgtatct attcctcctt tttgtcttcc aaattgccaa gcagctcctt gttgacctgt
    23581 atatggattg gtaacatctg cagcgataat caattttact ttagaaacaa gaacgtttct
    23641 tcgatcaaaa tgaagttttt tagtagataa taaaggattt ccttctgtgt acccttctaa
    23701 agaagcaccc aaggattgga gtaatcctag accaccatcg ctacacccgc ttcctcctaa
    23761 agtgacaata atttgttcta cttcttgaag cagtgcatct ttgataagtt ctcctaaggc
    23821 ataactggaa gcaagtcgga tagtttgatc cgttggttta tcaagtagat ctaaccccaa
    23881 tgcttgtgca gattcgatga ctgcggtatt ttttccttgc caagttgtca ataaatagct
    23941 gatttttcgc tgacggaata aaagatccat cccatcaatg gtgcgccatg ttcccccgag
    24001 cgcgtaatag attgcttctg cagtattttc tccgccatct gcaatcggca cattgatttt
    24061 tcttccttta cgatcttccc agccatttag aactgcctga tttagttcaa atgaagaagc
    24121 acttccttta aatgaatcaa ttgccgccaa aatatccatc tgtatccctc actttcttct
    24181 taagtaagac actttttttc atttttttca aacaaaacaa tgctattttc ttccattttt
    24241 ctatatacta aaagggaact cattgataaa ggagagaaaa aacatgaaaa tcgcaatagc
    24301 tggagccgga gcaatgggca gccgaatcgg attgatgcta catcaaagcg gaaatgaggt
    24361 cctcttgatc gatcgttggc ctgcacacat tgaagccatt cgtacaaatg ggttgattgc
    24421 tgactttaac ggtaaagaag tggtcgcaaa actgccaatc tattcaccag aagaaatcat
    24481 cgaaagcaac gaacacgtgg acttgatcgt agcattaaca aaagcgaatc aattagatga
    24541 tatgtttcgt tcgatccaat ccattattac tgacaatact tatgttttgt gtttactaaa
    24601 tggattaggt catgaagatg tactggaaaa atatgtgcca aagaaaaata tcttatttgg
    24661 gatcacgatg tggacagctg gtctagctgg tcccggaaaa gtcacattgt taggtgatgg
    24721 agaaatcgaa ctagagaact tggaaccaga aggcgaagca tttacaaaaa aagttgtgga
    24781 agtctttcaa gaagcaaaat taaatccgat ctatagccat aatgttcgtt attccatttg
    24841 gcgaaaagct tgtgtaaatg gaacattgaa tggtttatgc acgattttgg attgtaatat
    24901 tgctgaacta ggcgcgcaaa aaacggcaga gagcatggtt cgaacaatcg tatctgaatt
    24961 tgcttctatc gcagcaaaag aaggaatcat attagatcaa gaagaagtgt accaacatat
    25021 tgcttctact tatgatcctg acaacatcgg gttgcattac ccatctatgt accaagacct
    25081 gatcaaaaac catcgtttga ctgaaatcga ctacatcaat ggtgccgttt ggcgaaaagg
    25141 acaaaaatat gatattgcta caccatattg tgcctttttg acccaattag tccatgcaaa
    25201 agaaggaatc cttggcgcag aataaaaggc tctctgatga gaatcagctg aatcagcagc
    25261 tggttctctt tttttaagcc caatgaaaga tcaaaaagaa aacacaactc tcatgatgcc
    25321 ataagaattg tgcttgtttg tttcggtaca taatctcttc aaaatgtagg ttttttattt
    25381 attttcaaca gaggagaggt catattcgta ttgctcataa atagtggaat cgttagtgat
    25441 aaatcgtttt gcccaatttt ctgcatcata aaattccaat agctctgatg ggattggctt
    25501 aaaaggtgta tgcagaaatt ccggtaattc atctaaatca cgttctcgaa gcaaaaaaat
    25561 attttcagga cgataaaaat gataatgagc aatcgcttga ttatccgtca ctagtttctt
    25621 ttgaaaatac atagattcaa atgtccgcaa agtcactcct tgctgaccct cccgcaacag
    25681 ttccagaatc ccgcgcgatt cccctgtttt tttcaggtac tcttcataag caagatatcc
    25741 aggttgttca ttccctcgat gtggaagaat gaaatagttg gtcgtaacat ctctttttat
    25801 aaattcctgc ttatagcttt ctgctcgttc ttttctccca ttattcgttg caccaaaaaa
    25861 tagatccgtc tttggttcgc tagaagaaaa atccatatgt ttcgaataaa atgaactatt
    25921 atgtttcagc ccaagttctt ttgcttccaa aaaatcaaaa tgataaatct catcgattgc
    25981 cggttcatct tgtgcctgct tcagcatcgc ctcgtgttct gctttgaagt gattccaaaa
    26041 aaataacaca atttttgcat tggtaaatgg gcgcacgcgt cgagccatct ccatattaaa
    26101 tccataatct aaaaaaacga tcagctgata atttgccaac tgttgtctag ctgatttaat
    26161 gaattccgac tgtagtaaat cttttccgga ccaaacatct aaagagcgga tggaaggcaa
    26221 aaaataaagt gatttatttt ttgcaactag cagtacgtca tttttgttca tcttttccgc
    26281 ctcactttct ctgtcacttt ttcctatgat agcctgttta aatagtaaaa agccagtaaa
    26341 acgcttccta tatttgtatg aaaatttaac atttgtatga aaatatgatg aaataaatca
    26401 ttcttctatt aaaattcgcc taaaagtttt ctgaatagtt gtaattgtct gaaaatttgt
    26461 tatcattagt cattgttaaa tattgctatt tattcttaat ataaattttg attgtgaatt
    26521 taaaagaaag aggtcattta tggaagataa aaaattgaaa aaacaactta gctccagaca
    26581 tatcaccatg ttagctttag gtggagcaat cggtgcaggt ctatttaaag gaagtggcga
    26641 agcgattggg atcgccggcc cttctgtatt gattgctttt ttgattggcg ggctgatttt
    26701 atatatcgtg atgaaaggtc tgggaaaaat cgttttaagc ggtggcgaca cgcatcacgg
    26761 attatctggt ttgatacgcc cttatctagg tgctcattct gctgatttta ctgattgggt
    26821 ctactggtct atgtggatta tcaatatcat cgctgaagct gttgcagctg cttccttttt
    26881 gcagttgtgg ttcccgcaca ttccagcctg ggtatttgtt tttatgttgg ctgttttaac
    26941 tacgatcatc aatctttatt cggtacggtt attcgcagaa acagaatatt ggctggcttt
    27001 tgctaaaatc agtgtcatta tcttactaat catttttgct acttatcttg tcggtcagca
    27061 aatgttgggc acaggcgttt tccctaccct tcagcaattg actgaccatg gtgggtttac
    27121 tcctcatggt atgaagggaa tcatcagttc tttgcttgta gttatctatt cttacggcgg
    27181 ttctgaactg attgctatca cggtaagcga agcagatgat cctaaacgtg cgattcctaa
    27241 agccatcaaa ggtgtcatgg gaagaatcat ttccttttac atcattcctt tgtttttgct
    27301 tttgattctt tttccttgga atactttagc aggcacgaat gtcagtcctt ttgtgatggt
    27361 atttgaaaaa atgaatatcc catttgcagc ggatatcgtc aattttgtta ttgtccttgc
    27421 tttgttctca tctatcaatt cgggtgtgta tgcatcttca agaattttgt tcttccgttt
    27481 gaaggatcga aaaggttcct ctagaaaact agctgtttta aacaagcatc aagtaccgca
    27541 acgtgctgtt ttattttgta ccagtgttct ttatcttggt gtagctcttt cttattttgt
    27601 cggtgacaaa ttatttggct atctggcagg ttccctttcg tatacagtat tattgatctg
    27661 gatcattatc agtgcggcat cctttgtact ggcactaaga aaaggaacat tatgggataa
    27721 aggaatcagc ttttttgcat tagctgtctt aggattgatt ttccttggta tcctgttcac
    27781 gaactccatt ggggtaactg tattgactgg cctgctttac ctgttcattt atttcagtta
    27841 tcaaaaaaag aatgatgcgt tcgtattgac gaatgaatga aatcgataga aaattttatt
    27901 ttatttatgt ggaatagatg gaactattga agaaaggagt tgtgacaaaa gtcttgttgc
    27961 aactcctttt tccgaataaa cagtctcatg acctcttggt aaacggtgtt caaaaaccct
    28021 ctgtgcaaaa taattgattg ttctccatgc tcatgacatg atagaagaaa ggaatgaaaa
    28081 aaaggagttt gttttaaatg aaaaagtatt ggaagttttt gttgatgatc ggtgtttcaa
    28141 cggtagttat gtttggtatg atgtacttga atgtctatgc gctcgatcat atcttcttca
    28201 gtcgtacacg agtattcatg gcgttgatga tgggtgcagt catggcagtc atcatgctgt
    28261 tgttcatgtg gaaaatgtat gaaaacaaaa agataaatgt tatgatctta gccgctagtc
    28321 ttcttgtttt tgccggttcc ttgtttatgg tacgaagcca aactactgtc aacgacacag
    28381 cttggatgaa ggccatgatt cctcatcatt ctatcgctat cctgacaagt aaacgagctg
    28441 atttaaaaga tcctgaagta aaaaaattag cggaagaaat catccaggca caagaagaag
    28501 agatcgacaa aatgaaaaaa atgatcaaag aaatggaatg accattatcc gtccaaataa
    28561 aaaaagagga tgttccagaa gtgatcagct tcaagaaata agaagaagct acttctggaa
    28621 caccgtgaat aaggggttgt gaaaaagctg ccctgcatca agtaataaga acagccaaac
    28681 aaaaatagct tctcctattt ttcgtttggt taggcttatt accgcagatg caagcttttg
    28741 aacaccgttt attcgtaaaa gggattgtga caaaaaaagt cttgtcacaa tcccttttta
    28801 tgtttttatt gttctaattg cgctgtcttg cttactgctt cttgcggttt cacaaatttt
    28861 tttgagattc cccaacctgt gaaggcactg accatcgaaa acacaggaga aagcaaactg
    28921 aaaaatacga atggcaagta agtgagtgta ggaataccaa atgtacttgc tacgaaactt
    28981 ccggcaattc cccaaggcac taagtagttg attactgttc caccatcttc tagcgttcta
    29041 cctaatacag ttggatcaaa tcctgctttt tcataagttt ctttaaatgc attaccaggc
    29101 agaataacag ataggaattg ttcaccaata aaaatattta ctccgatact tgtcatcaat
    29161 gttgtaaaaa tcaattttgg tgtgttcgtc atctttcttg atactcgttc catcacggtt
    29221 gagatcaagc caaattcgat caacaatcca cctaatgaca aagttagaat aatcaatgac
    29281 acagtaggca tcatactttc gattcctccg cgactaagca gtgaatcgat ttgttggttt
    29341 ccagtatttg aaacaaaccc tgattcgatg accccagcca acttcgttaa ggaagtttgt
    29401 gggttttgaa taaaaatcat tcctactgct acagcgatat tcaacaaaat cgtcaaaatt
    29461 gccggcacct ttttccatgc acacagtaac attaaaaata aaggaatcag tgaccagatt
    29521 gaaatagtaa agtttgcttc tagaacatca gtcacttcac gaatagatga aagacttgcg
    29581 gctttacttg tgtgccctaa aatcatgaat aaaaagaaag aaacaacaaa tgccggaaca
    29641 gttgaccaca tcatatgctt gatgtgtttg aacaaatcgg catcaacaat agctgctgct
    29701 agatttgttg attcggataa tggagacatc ttatctccaa aaacggcacc tgaaacaata
    29761 gctccaacaa ccagcgctgg atttaggcct agtgttgtcc caataccgaa aaatgcaatc
    29821 ccaatcgttg acataacagt aaaggcactc cctacagcgc tacctacgat tgcacacaca
    29881 acaaatacag aagggacaaa ccactggaca ctgatcaatt tgaacccaat gaccattaga
    29941 gttggaatga tccctgcttg tatccataca gcaatcaatg aacctactaa aataaaaata
    30001 aagattggga tgattcctgg tttgatgcca ttgatgattc cttcgtgcag ccaatctgtc
    30061 gggtatcttt ttatcactgc atacaacatg accattccga tcgctgctaa gatcgtgata
    30121 ttaggtgaga cctttaatcc gacaacgcca atcgcaatgc ctaaaacaat aaccattaat
    30181 acaagtgttg cttctaaaaa acttggtgtt cctttttttc ccatgataat ctctcctttt
    30241 ttagttgttc ttatgttcta aaaaagttaa gatatcggat tactccgccg caattcatcc
    30301 gctattcctc ctttataaaa ataaaaagac ctgttttaaa ctaaggacga atgactccgc
    30361 ggtaccacct taattaaaaa cagatctgtt tttcactttc aaacttttat tcaaatgtat
    30421 ggactttcca tgtaattcgt caattaggct tgatcggttc acagcgacca ccgactttct
    30481 gaacaaaaca cctaaccgct actgcgtgaa aagttgactt gtatgaagtt taccttccat
    30541 aagatcattt gtcaatgaaa aagaaaatat tttttcattt ttctgaaaat tatttttata
    30601 actttcacac acaagtttat tttttactag aaagtagcac cctcttgttt ttttcttgct
    30661 actatttctt gatgttcttg cttgattctt tctaatagct ttggctcttt caacagatca
    30721 agagctgtat tggcgagtag ttctgctgca atcgcaatag aagcaaggcc tttgtcactt
    30781 ttagcagcag ctttgaattc ttctgtgtgt ccggcaatat atgtctcaga gattgctacc
    30841 gtaggctgga ttgttggtat gacttggctg acatttccta catcagaaga accgagactt
    30901 gttctttgtt ccgtttctat ttcttcatct ggaacacctg cttcttttgc atgctggaag
    30961 aataagtcat caaaagaagg agtaacgatg acatcatcaa ctgcattttg gaacaatcca
    31021 aactcaaaag tcgtgcccgt acttatcgca gcacctcgta cgatattttc cactttttca
    31081 tatactttat ctaatgtttg ccgatttcct gcacgtaaat agaaacgtcc agcagcgtaa
    31141 tctggaatga cgttggcagc tactcctccg tctgtaatga tcccatggat attgacatct
    31201 tttggcaatt gcaagcgtag agcgttgatt ccattaaaca cttggatcaa tgcttccaaa
    31261 gcatttactc ctttttcagg tgctgctgct gcatgggaag cgacaccgta aaatttgatg
    31321 tcgactggat cattcgctaa agattctgtc gttttcccat aacgaaaagc tggatggacg
    31381 cataacgcta catcgacatc ctcaaaaaaa ccttctcgaa caaagctgcc tttagctgat
    31441 ccgttctcgc ccccttcttc ccctggggtg ccatatacac ggatttctcc accagtttca
    31501 tcaataaatt gctccactat gctggcggct aatacggagt aagtaccaaa caaattatgc
    31561 ccgcatgcat gaccaatacc tggtaaagca tcgaattctg ccaaaaaggc aattgtgggt
    31621 ccgggtttat tggaaatata acgtgcatca aaggctgttc ggtgtccagc tacttctttt
    31681 tctacccaaa acccttcttt ttctaattgt ttaatcaacg ctgctgagga ataaaactca
    31741 tagttactta cttcaggatg ttcgtggata tctagagcaa gtgcttgata ttctggtaaa
    31801 cgatattgga ttagatcctt caattggttt gttgagattg ttgttgtcat aaaaaattcc
    31861 tcctatgcta gtcatttttc ttattttctc ccgaatgttt cccatgcagg cacgcttgat
    31921 ccttttgatg ttttctcgat tactttgact gtgtcttctg tttgataagc gtctactatt
    31981 ttttgatacg ttttattttt ttcatcttct tttcttgcaa cgatgatatt cacataagga
    32041 cgtgcatggt catccactgg ttctaagaag atggcatcag aagcaggatt aaatccagca
    32101 tctacagcta caccgctatt gatcactgaa atgtctacat ctgacaaagc acgtgctgtt
    32161 tgggaagcat ctaattcaga aatgtctaga ttttttctat tctcggtaat atcagaaaca
    32221 gtgggcgctt gtccttttgc cttatccact ttgatcaatc cagcagtttg caataacaga
    32281 agagctctgc cgccatttgt aacatcatta gggattgcta cttttgctcc gtctttgata
    32341 tcagctacat ccttcaaagt gttagaataa attcccagcg gtgcattgac agtatttccg
    32401 atcgatacta aatcagttcc gtgttcttta ttgtaattgt ctaaaaagaa ttgatgttgg
    32461 aaagaattca aatcaatcga tccatctgct aaagccgcat ttggttgatt gtaatctgta
    32521 aatttcacgt attccaaatt aatgttctcc tttttcaaac ggtctttcac gttgtcccat
    32581 acatctgtat cttctccaat aattcctagt tttacggtag tttctttttt gtcagttgta
    32641 tctgcttttc cttggctaca ccctgccaac caaataattg ctacaaatgt aatgactaca
    32701 aaacgaataa tttttttcat tttttctcct ccactttcaa tgtgtaattt ttttaatcag
    32761 ttgattactg ataaattgac ttaaaaaaac taagattaga atcaataatg tagccacaat
    32821 cgttacatct gtttggaatc gattataccc tctcgatata gcaagattcc ctaatccgcc
    32881 tgcacctact gctccagcca tggtagtcag tccgatcacg ttgatgatag tcaacgccga
    32941 gacccgaatg ataccaggca aaccttctaa caaatacaca cgtaaaataa taccgattgg
    33001 actggttccc atcgcttctg ccgcttcgat caccccagga tcaacttcta gcagtgcgtt
    33061 ctcgatttga cgagcaaaaa acggaacaac tccaaaaatc aatggcacta aagctgcttt
    33121 cgcaccaatc gtcgttccaa ctaagaaacg agtagtcaat gctaaaagag aaagcaagat
    33181 aatgaaaggg atggaacgaa tgatattgat cagcttatca agtatttcaa atagcagacg
    33241 gttagctagt aatccattag ggcgtgtcac tactaacgtc acacctaaga tgattccgat
    33301 catcccgcca atgaatgctg tccaaaaaga catatatagt gtttgcttcg tcgcttcgac
    33361 aaattcatcc ggaatcaaat aaacattggg caaatatgtt ttaaagaaat cagtcatttt
    33421 atcttcctcc ttcgatcact ttgatacgac tatctaattc gtttttttgt aaaatattta
    33481 tttttacacc agatttgatc aaataatcca atgctttttg ccgctgtttg gtttctccag
    33541 ctaaagtaac gatcaaactc cctaacggcg tttcttgtaa taactctaca ttgccatata
    33601 aaatattcgc tgttacttga tatttgctgt acagatgagc gattagaggt tcgttcgtct
    33661 gatttccaat ataacttaat tccaccagcc atttattggc gatctgatcg gcaaaagcag
    33721 aatgggcaat gatcgtttct aatgcttggt caagatgagt agctgttcga ataaaatctt
    33781 tggtcaattc ttcttcagga ttgctgaaga tctggatgct actacctttt tcaatgattc
    33841 ttccatcttc cataaccgct accttgttgc agatttcttt gacaacttgc atctcatgag
    33901 tgatcaggac gattgttaaa cctaactggc gattcaattt ttttaacaat gcaaggatct
    33961 gaagggtcgt tttcggatca agtgcactgg ttgcttcatc acataataat accttaggtt
    34021 cattcgccaa agctcgtgcg attgctactc gttgtttttg tccccccgac agttgtttag
    34081 gataagcaga tgctttttct tccaaaccga caagttccag tagttcattc actttttgac
    34141 gacgttcctg tttcgatttt ccagaatact ttaaagaaaa atcaacgttg tcaaaaatag
    34201 ttcggctttc cattagattg aaatgctgaa agatcatacc gatggccttt ctttcttttc
    34261 gcaattcttt gggagagagc tgactcaaat tcgtctgatt gacaatgact tctcctgctg
    34321 ttggtttctg cagcaaattc atcacacgaa ctaacgtact tttccctgca cctgagtaac
    34381 cgacgattcc ataaacatcg cctttttcaa tcgttaaatc tacagaatct actgcctgta
    34441 tctctttttc tttttgtttg aaacatacag aaacatctcg caattcaatc attaaaattc
    34501 ctccttatct aaaaaaagcg tcctcaagaa aatgaaaatg tcattttctt gaggacgctt
    34561 ttttgcgtgt taccacctca tgttcaagct gtgttcacac acaactcctc ttcaagtacg
    34621 atcatactct agcaagatat cggtcgcagc cgtcatcgcc ttacatctat gctcgggatg
    34681 aattgctcga tggccatttt ccaatagtcg ttccttgctt cttctcagct accgaagctc
    34741 tctgtcaaag gttcttctat cgtacttacc atttcaatgc aaataatatt caaaaaaaaa
    34801 tccttttact aacataaact atgtcagtaa aaggacgacg tatcgcggta ccaccttttt
    34861 tcggaagttt ccttccctca agcccaacat ctcaaaaaga gagattggcg ttcgttttaa
    34921 cggtcgaaca gttccgaatt tgcctactta tcgacaaatt attctccaag gccattttca
    34981 gcttttctta cttgactctt ctcaccagcc agagcctctc tgcaaagttt ccaaacttac
    35041 tttccttttc aacgaaatcg atattttgtt gttgggatta atctacatga ggaaaactaa
    35101 aaagtcaagc acgaaattca aattattttt tctatattta tttttctgtt tttttatcta
    35161 acatttcaat aacagttcga agatcttttc cttcagcaag aagtttcaat ttttcctttt
    35221 cttcgacggt ataatttcgg acaaaactgg aatcaggtat caaaacaacg cctaatcctg
    35281 ctcgtgaagc agctgttgct cctactaaag aatcttccac tgcaaaggct gaactagctt
    35341 ctaatcctgt tcgttccaaa gctgtcagat aaggaatcgg tgaaggcttg ttctccgaca
    35401 cttcgtctcc gaatgtaaac gtgtcaaagt aatcagctaa tttaaagtga tccaagatat
    35461 ctgttgctct tttcgtaaca gtggtcgtag caatgcctag tttcatatga cgttctttta
    35521 agtctgccaa tatttctttt gcatatggtt ttaagtggat ggctccgctt gtcagctgtt
    35581 gataaaaata ttcttcacgc ttcgctcgga cttcctgtac tttttcaggg ctacctgctg
    35641 ctttcactaa aatatcaaaa ctttgtttcc agttttttcc gccccaagcg tcaacgattt
    35701 cttttgggat atggatatca aattgctgaa atccccaaag ccagccatca cgatacgttt
    35761 tttctgtgtc aactaacaaa ccatctaaat caaatattgc acctttcatc tatacattaa
    35821 tcctttccaa aacaatacac atagtttagc acgactggtc caaaaataac agaagcaaag
    35881 aaccagcctt ctcgtttata agagttcttg gaatcgatgg atatagattt tcttcacagc
    35941 tgtcacaagc gccagataag cgatgactgt agcaaataac cataaccagt aattgccagg
    36001 aagtggcatc aagcctaatt cagcaccaaa acttgtaaaa ggaaggacag aaccgacaat
    36061 aattcctaat gtagtgataa caaacatggc aaatgttacg ttgctttgga taaatggtac
    36121 tttcggtgta cgcagtgcat ggagcactaa ggtttgtgtc catagtgatt cgacaaacca
    36181 tcctgcatgg aataatgcga tgaaaatcac ttgctgtgaa ctatccaatg tatggaaact
    36241 tccgccaact acagcaggac aaataacaaa atacatcaac aagtaagtag taatatcaaa
    36301 tatcgaactt gttgggccaa agtaaaccat gaatttccca atactagatg cattccattt
    36361 ttttggctcg tccaaatatt ctttatccat ccgatcccat ggcaaggaaa tgcaggaaac
    36421 atcatagatt agattcaaaa acagaagttg taaaggcagc attggtaaaa atggcaggaa
    36481 catacttgca atcagcacgg aaaacatatt gccaaaatta gaacttgctg tagccttgat
    36541 atatttcatg atattcccaa aagtttctcg tccagaaaga aggcctcttt ccaaaatcgt
    36601 caaatctttt tctaaaagga tcacgtctgc agattctttc gcaatatcca cagctgtatc
    36661 tacagaaata ccgatatcag ccactttcat tgcaggagcg tcattgatac catctcctaa
    36721 aaatccgaca gtatgaccat ttttacgtaa agctgtcgtc aaacgtgctt tttgctgagg
    36781 attcaatttt acgaatactt catggtcttc agctacttgt gctaattcat gatcactcat
    36841 ttccgagatt ttctcaccgg taaccaattc attgatctct agacctactt gacggcacac
    36901 tgaacgtgta actaattcgt tgtccccagt cagaactttt acagctacac catgttcttt
    36961 cagagctttc aacgcttgtt tcgttgtttc ttttggtgga tcaaggaaag taagataacc
    37021 aatcaaaacc atgtctgact catctttgac tgaaaattct ccaacgacgc ttggattggt
    37081 cttttgtgcg acaccaatca ctcgtaagcc atcttcatta agtgactgta cttttgcaag
    37141 tacggcttct tttcgttctt ttgttaatgg tgtaacagta ccgccaagat caataaattt
    37201 agaaatgttc agcatttctt caatcgcgcc ttttgtgatc atttgtgttt taccagctgg
    37261 atcagcaacc acaacactca tacgacgacg ttcaaaatca aaaggaattt catccacttt
    37321 ttgataattg atcttatctg tatctaaagt ttctgttgct tcatcaatga tagccacatc
    37381 taacaaattt ttcagccctg tttgatagta gctatttaga tacgcatggc gcaaaacacg
    37441 atcatcttca tttccttcac aatccatatg atattcaaga atgattttat cttgtgtcaa
    37501 tgtccccgtt ttatccgtgc ataacacatc gattgcacca aagttttgga tggcattcaa
    37561 atttttgatc actgttcctt ttttggccat cacacttgct cctttgacca aattcgtcgt
    37621 aacgatcatt ggaagcattt caggtgttag accaactgct acagatagtc caaaaagaaa
    37681 agcttctagc caatcccctt ttgtaagccc attgatgacg ataactgctg gagccatcaa
    37741 tgacataaat ttgatcagta gcatggatgt tttatggatg cctaattcaa agcttgattg
    37801 aattggtttt tcagatagcg tttctgctac atgaccaaat agtgtttcat ttccaacaga
    37861 aacaatgatt ccttccgcac ttccacttat gacattactt cccataaaga caagattttc
    37921 gtaacttgtt tcgctttgct gatcgtcttc acatactttc gcatgctttt ctactggata
    37981 gctttcccct gtcaatgctg cttgggaaat gaataaatcc tttgcggaca ataaacgcat
    38041 atccgctggt atcatatccc ctgctgaaag ccgaacaaga tctccacaga cgatttcttc
    38101 cataggaatc tctgtgtact ttccattacg ttttacagct gccgttactt tcacaagtga
    38161 tttcagtttt tcagccgctt gattggaacg tacggactga atcaacgtca ttgtcccgct
    38221 gacgatgacc atagtgaaga taatggctac accgaaaaga tctttatctc caggagctgc
    38281 taatacatat tccgtgatga atgaaataat tcctaatgcg atcaacacta atgtaaatgg
    38341 cgtgatgtat gctttcaata tttctacgat gaatggtgtt tttttgccat atgaaatcgc
    38401 attttcaccg taattttctc ttgaacgttc gatttgttct tctgtcaatc catttgctga
    38461 tgcgtggaat agttcaaata attcttgttt cgtttttcca gcaaaagctg catagatttt
    38521 ttgtgtatct ttcttttttt ccatctcaat ctctcctttg tgtccaaaag acagctgttc
    38581 tcgctgcttt tggtctttag ctattcatcg gatcgataga gaaaaaactg aaaggggctg
    38641 agaacagata gataatagcc ttggcctttc ctctagatcc ttgtttttcg aatgactgtc
    38701 atccatttgt tctcatctcc tttactaagt aattttaatt catgacttcc aatcattttt
    38761 cgaatagtta ttttcccaat tagaccacct cctttttatt tttcaccgat ggggatttgc
    38821 ggcaacaaaa aaacctctcg ccaaatactc agcgaaaggt ttgaaaaagc atattaaaaa
    38881 aagcttctca gatctcatcg ttgagttttg acactgtaca acgtaaaaag cagatacctg
    38941 cttttagcta ccttatgaaa agccttatct cgacaattcc tgttctaccc attggcgtct
    39001 ctcgacgttt ttgggcagta gcctatgttt gtataggagc ctcacctaac aaatcttatt
    39061 cggttttcct tcgttaatat aacaactaaa aaagaggatg tcaaatcaat tttccatgtt
    39121 tttcatactt tttttaaaat acacacgtta tattcctgtc tcacgcggtt ttatttgtct
    39181 cattttccac ctcccgctat aatagaaata tagtaggaaa cgaggtgctg ttatggaaag
    39241 aacacttgtt attatcaagc cagacggcgt gagacgccat ttagtcggaa gcatcattca
    39301 acgttttgaa gcaaagggac tggccattgc tgaaatgaag tttgaaacta tgactcctga
    39361 attagcaaaa gaacattatc agcacttgac tgagcgcagt ttttttgatg aactgatcga
    39421 ttacatgact tccggtcctg tcgtttatct tgtgctggtc ggcgaggaag tgatcgatat
    39481 cgtccgtaag atggttgggg caacaaaagc tgtcgatgct gttccaggta cgatccgagg
    39541 agactacgcg ctgccgggga ctgaaaacat cattcacgct tctgattccc gtgatgctgc
    39601 ggtaaaagag atcgcccgct ttttcccgcc attcgatata aacaaaccgt ctgaaaaagc
    39661 ggcgaagtga tacaaattct aaactgctgg tctagttgct gggttcagca atggccagca
    39721 gttttttctc taaggacctc cgttgattct ttcctaacat atattcgagc actgtgaaga
    39781 attcttggta gtctttcgtc tttttttccc ctaaagcagc agaaatcaat ctttgataat
    39841 aatctgccat caatgtttca tagatcactg tacttctttc tttatgtgct aagctccgtt
    39901 tcatatcttc attcacacta cgtgcctttt tcaaccgacc attttctata taaaacgaag
    39961 tcaggttcat taaaaaagta aagagatact gctcatgatt gttcaactgt gctaatcgtc
    40021 gcattctttt tttgattttc aaataattgt tctcaatgaa ccgatcactg aatatccaag
    40081 gaaaatcagc tagaatagat agttcaaatt gaccatagtc tggtatctgc atcaagtatt
    40141 cttcaatgat tcggcattca taacgtaaaa attcaaattc tgtctcagat acttctgttt
    40201 tttcgaccat gtgaagactc aaaaccaaat aatacagatc ttctgttttc ttaaaatgcc
    40261 tacggttatt gctgatctct ttataaggat caaagctgtc ttcttgtacc cgatctagca
    40321 ttcgtctttt tttctttaac tttattggtt catccagctg gtttcgcatc cattcatatt
    40381 ctgcaagtgt catgttcatt cgctctaaaa atgatctcaa tgttacaaat gacatttttt
    40441 tcactgaatt tgtctcaatc ccgactagag aagtcttaga acagattcct tccgctaatt
    40501 gggactgtgt caacttcttg ttgactcgaa gtttcttgat caattcacca tcatacataa
    40561 tagttctcct ttttaattta aatttttcca cattcaataa atagatacgt taaaaaggaa
    40621 aaatacacga aaaaaataaa aaaatgtttt tttcataaaa aataccaacc ttcaagcatt
    40681 agaatgttgt ctaactactg aaggttggtt tgattgttta ttgattatct ttttgctgtt
    40741 caacgtctgt tcttgttact ggatataagt tggttgttgt tttattgcca ttttttgaaa
    40801 agacacttgt cgaatgttcg cctttttgct tttcgatggc tatgttacgt tctgcttcat
    40861 ttttatagtc atattttttc ggatcgatcg tttcaaatcc gtttggcaca taaaaacgca
    40921 agagattttc ttgattcagc ttatctgaaa gacttaaagc agatttgact ttttcctgat
    40981 ctttggaaat ttcctcgtcg attcctgctg ctttcgtatc gatgatttct cctgtttggt
    41041 tattgtaagc ttttcctcct aagatggtgt atttgggtgt cacgaagttc ccatttcgaa
    41101 aagccactac ttgatcatgc tgaggagata ataaatccgt accaaaatgg agatagtctt
    41161 tatcatcgat tcccaagaga tgaagtaatg tcggcaaaac atcgatttct ccaccgtatt
    41221 cgtgattgac tgacccttta gtataaccag gtatatgaat cattaatggc acacgctgca
    41281 tttgtgcgtt gtcaaattca ttccatgtat ctgggtcttt tccaagagca cttgctaagt
    41341 ctttattatc tgtatttgag atgccaaaat gatccccata aatcacaaag atcgaatttt
    41401 ggtaaatagc ggattttttc aggtacgtaa aaaattgttc caatgactga tccaaataat
    41461 gtgccgttcg aacatagtta tctactgtgc tatttccagt gttcaaagat ggaaaatcga
    41521 aattcttatc atccgtataa taaggcgtgt gattggttac ggaaaggaat ttcgtgtaga
    41581 acggctgttg tagatgttca agatattggg cagattcact gaataaatct ttatctaata
    41641 ttccataacc tagcgtttca tctgattcat caaaataaga tcgatcaaag aaattgtcat
    41701 aacctaagtt tttatataca tgatctctat tccaaaaact agctacattc ccatggaaaa
    41761 cagcgcttgt atagccttgc ttttgtccaa ggatcgctgg tgcggcttga aagacattat
    41821 ctgacccaag ttctgtgaat aatgagcctt gaggtaaacc aaatgtgcca gtttccaaca
    41881 tattttcagc atcacttgtc tttccctgtc ctacttcatg aaaaaaatta tcaaagctga
    41941 tcgttgcttg attctggaaa atactattta aaaacggtgt gacttcttgt ccgtctacct
    42001 tcatattaat caaaaactgc tggaaacttt ctaaatggag aacgatcaca tttttccctt
    42061 tagcgatccc aaatgtttcg ggatttggct ctgcataatg tttctttgta taatctaaaa
    42121 cttcatcaat cccattactg cttgcatgcg cgcgaacact gctagtcatt cctgttttga
    42181 ttccatcata aatagtatac gcatcgattc ctagatattt gacaatatat gaacggtcaa
    42241 atgttcgctg cagcaattgt ggacggttgg cttcgctcag cgctagattt cctgaaaaaa
    42301 taagccctga aagacaggtc acagcgatag ccatcggttt gctgacaggt tgacttttga
    42361 taggtatttt cttgatcgtc aaccaaacca gtatcccaat gaacgtagca atatccagcc
    42421 agtagaggac atcatggggt ttcatcaaag aaaaggagct tcctgataac ccttgcgaca
    42481 ctttcgaaaa tcccaataca gatttgactg taatgaaatc tgtgaattcc cgataaaaaa
    42541 taatgttcaa gtacaacaaa agcgtgttta aaatatcaat gatcaaaaga atggctaatg
    42601 ctggctttgg ctttttaata tacaaagaaa ggccaaaaaa caataaggta gttgcaatcg
    42661 ggttgatcca caagataaag tactggatcg taccttccac acctaacgaa aaatctgcat
    42721 agtaagcaat aatcgtcttc atccagaaaa agaaaacagc tgtcaaaacg attgctaaac
    42781 gattttggca caatttttta agttgtgtca cgtatatcac ttctctctat tcacattttt
    42841 ccttattttg ctcctgaaaa atgaaaattc cctaaaaaag ttgttatctt aagatttttt
    42901 tattctttct aaagattatt ccgtgcactt ttcacagcat aaaaccgctg tgatataagc
    42961 gattgaaaca gtaaggtgat aaaagtcttg tcgcagctcc tgtttagttg atgcaggaca
    43021 gctttttcac aacttcttct ttaaacggtg ttctcctgcc aactcctcgg ccacaagtca
    43081 caatatttat aaaacatgag aagctgtttt ttgaaattgt tccttgtggc tcagagttaa
    43141 acggcatgct caagacctct tgata
//
LOCUS       DACGLE010000015        42895 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0534
            SAMEA3304449-rid9560453.denovo.016, whole genome shotgun sequence.
ACCESSION   DACGLE010000015 DACGLE010000000
VERSION     DACGLE010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304449
            Sequence Read Archive: ERR985064
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 42895)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42895)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702225.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702225.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 20:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,045
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 27 of 67
            Pseudo Genes (incomplete)         :: 25 of 67
            Pseudo Genes (internal stop)      :: 19 of 67
            Pseudo Genes (multiple problems)  :: 4 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42895
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304449-rid9560453.denovo.016"
                     /strain="VREN0534"
                     /db_xref="taxon:1352"
     gene            214..498
                     /locus_tag="IXT20_000956"
     CDS             214..498
                     /locus_tag="IXT20_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1905 domain-containing protein"
                     /protein_id="HAQ4859423.1"
                     /translation="MREIYSFESKIYASEVGKGGAYIIFPYDIRKEFGAGRVKVQATF
                     DNVPYEGSIVNMGVKNPDGSVCYILGLRKDIRKNIGKEIGDIVAVTVKQK"
     gene            complement(779..1198)
                     /locus_tag="IXT20_000957"
     CDS             complement(779..1198)
                     /locus_tag="IXT20_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid decarboxylase"
                     /protein_id="HAQ4859424.1"
                     /translation="MEKETIVEVTKLGFTIQAAVKVIGKDLLITLTGGDTPHIGTVTT
                     FSRDTEMQTVRFPSHDGRFHKDDVLAEKIAVIIKTAVPGSCTITSGIHVDHISNAQIN
                     ASFPMSEELGYKILDWLEKIDFHIKEPVYYKDGEKPL"
     gene            complement(1200..1784)
                     /locus_tag="IXT20_000958"
     CDS             complement(1200..1784)
                     /locus_tag="IXT20_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HAQ4859425.1"
                     /translation="MEQLAQRKKKIVIGVSGASGTIYAINLVKKLKEYPMIETHGIIS
                     PWAKQNLKLESDLSLTEFESLFDYHYSNKDLGAAVASGSFLTDGMIIVPASMKTVAGI
                     SVGLGDNLIARAADVTMKEQRKLVIVPRETPLNAIHLENLTKLARLGVQIIPPIPAFY
                     NQPQTIQDLVNHHTMKLLDQFEIVNEHSKRWQGD"
     gene            complement(1831..3306)
                     /locus_tag="IXT20_000959"
     CDS             complement(1831..3306)
                     /locus_tag="IXT20_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HAQ4859426.1"
                     /translation="MTDQPYDLRKVIEELKTLPGQYHETDIEIDPEAELSGVYRYIGA
                     GGTVKRPTQEGPAMTFNNIKGFPGTRVNIGTMASRKRVGEILHHDYRTLGRFLKDAVE
                     NPIKPVKVDKADAPAQEVVHLATDADFDIRNILPAPTNTPEDAGPYITMGVVLGSDPE
                     KTMSDVTIHRMVLEDKDTIGMYIMPGGRHIGHFQKQFEALNKPMPITINIGLDPAIAI
                     GTTFEPPTTPLGYNELWCAGALRNEPIQIVDGIAVDEVGIARAEFIIEAEIMPNETMQ
                     EDINTNSGKAMPEFPGYTGDANPAVQVVKVKAVTHRKDNPIMQTTIGPSEEHVSMAGI
                     PTEASILALVDKAIPGKVVNVYNPPAGGGKLMTIMQIRKENEADEGIQRQAAILALSA
                     FKELKTVILVDEDVDIFDMNDVVWTFNTRFQGDKDIIVIPGMRNHPLDPSELPEYNPG
                     SIRFRGISAKTIFDGTVPFDMKEHFVRAKFQEVEDWEKYLK"
     gene            3487..3798
                     /locus_tag="IXT20_000960"
     CDS             3487..3798
                     /locus_tag="IXT20_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4859427.1"
                     /translation="MKKMEESFPKAVKVENIANILKVTFENGEVKYVKSHWTEEITDA
                     LQFGKKGRGKRKNLLALSRNMWIGTEVTIEADGTVFINGKDRYTPEELWYKGKKSIPE
                     L"
     gene            complement(3913..6249)
                     /locus_tag="IXT20_000961"
     CDS             complement(3913..6249)
                     /locus_tag="IXT20_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ4859428.1"
                     /translation="MLQLKDIKKYYKVGETTTKALDGVSVAFRQKEFVAILGPSGSGK
                     TTMLNVIGGLDNYDSGDMVINGKSTKDFKDSDWDAYRNNSIGFIFQSYNLIGHLGIIE
                     NVELGMTLSGVSKDEKRKRAEDALHRVGLTNHMHKKPNQLSGGQMQRVAIARALANDP
                     DILLCDEPTGALDTETSIQIMELIQELSKEKLVIMVTHNPELAHQYADRIIEFSDGKI
                     LTDSHPHIERPKDDQFNLRRTKMSFWTALKLSFNNIRTKKGRTFLTSFASSIGIIGIA
                     IVLSLSSGFQKQIDNTQAETMAKFPITISKVTTNQTRDDAGLGASKADYPDSKTITAK
                     VSEEDKAQHTNKIDQTYVDYVTDIDPNLSNNIGFTRTTGINLLRDVNGKVQPVSFSNQ
                     NPDTESLSLSSTMSAMTGVGVSSFPTQLDTSKENFLKDNYSLLAGSYPASATDVVLIV
                     DGNNNTNINALKNLGFDVKEDEKLDFDDIVGTTFKLVNNNTYYTKLPTGNFIPNTDYD
                     AMYQNASDELKISGILRVKSSSTMNLLSPGIAYSDQLTTQIVNENKESEIVKAQRDSG
                     VNVLTTEKVDENAKQTLLSYLGGDSLPSSIMIYPNNFEDKEKILDYLDDYNKGKSDED
                     KIIYTDLAGTMTELTGGLMDAITYVLIAFAGISLVTSMIMISIITYTSVIERTKEIGV
                     LKALGARKKDITRVFDAETCILGISSGILGVFIAWLATFPINSILYNMTDLKNVAQLN
                     PVHAIILVIVSTILTMLGGHLPARMAAKKDAAIALRAE"
     gene            6391..7059
                     /locus_tag="IXT20_000962"
     CDS             6391..7059
                     /locus_tag="IXT20_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ4859429.1"
                     /translation="MNRILVVEDDPQLQLLYRSVLERAGFSVITALNGEDALKKLEAT
                     RIELIITDIMMPKMDGYDLLDSLRSTRFDTPVLMITAKADFEDKKRGFKLGADDYMTK
                     PIDVNEMILRVEALLRRSKINYSSSLIIGQTKLELDAYTVLQDQHSLILPQKEFLLLY
                     KLLSYPNKIFTRQQLMDDIWGLDTNTEERTIDVHIKRLRTRFEKTEDFQIITVRGLGY
                     KAVI"
     gene            7062..8105
                     /locus_tag="IXT20_000963"
     CDS             7062..8105
                     /locus_tag="IXT20_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAQ4859430.1"
                     /translation="MKKKLFSQLWIYFASIVFISILVTLLCFLGFIFLLSRHSISPAE
                     SAGKPTLYPLFVFAGFSMVVGTGISIFVGRRILHPISALGTNMSLVATGDFSIRMDEQ
                     QKVAEVQQLYKDFNVMVQELNSIETLRNDFVSSVSHEFKTPLATIQGYVQLLQAPNLS
                     DEERQIFLYRIIESITQLSQLTENILKLNKLENQRIQLEKKEYRLDEQIREVIVFFQP
                     KWEKEQLELDIELAAVNYTGNEEFLYQVWLNIMDNAIKYNQINGQIHIKLFETATEIV
                     LEVTDSGVGMNEETRDRMFEKFYQGDTSRQISGNGLGLSLVKKILELHDGRIDYSSIE
                     GVGTTAMIRLSKQ"
     gene            complement(8342..9223)
                     /locus_tag="IXT20_000964"
     CDS             complement(8342..9223)
                     /locus_tag="IXT20_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAQ4859431.1"
                     /translation="MDIHKFEVFLDLAETMNFTKTADRQFTTQGNISKQILSLETELD
                     VKLFERAHRKIELTEAGSLLLPYVKNVVEQYHAMQEVLETYTKDNNLSLNILTIPTMI
                     NYKGFSKITEFLKRHPEFTVQLKEVESVELTYNDANEHSDTIHFARSFQAPPSSIEWL
                     PTETDDFVAVLPKNHPLASKKKLDLSELKKENFLLLGPTTNLYQPVLDLCREAGFEPK
                     ISYKGARIDLIINMIANDMGIAIVMEKTVKNLLKENTVILPISPTTESYLAFTRKVGE
                     HSLASDTFWEYLNQEEN"
     gene            9382..10110
                     /locus_tag="IXT20_000965"
     CDS             9382..10110
                     /locus_tag="IXT20_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HAQ4859432.1"
                     /translation="MPTGVLINVGSVLFGGLIGGLVGNKLSEHFKAQLTMVFGVCSMG
                     MGIYSIAPMKNMPAVIFALVIGTAIGLIVHLGNGINKGAALMQVPISKIFPSEKLGMT
                     HDEFISTLVTVIVLFCASGTGIYGSLDSGMTGDSTILISKSVLDFFTAAIFACNLGYV
                     VSVIAIPQFIIFYILFLLAKFIYPLTTPDMILDFKACGGFLMVATGFRMINVKMFPVA
                     DMIPAMIVIMPLSWMWTNWIMPLL"
     gene            10266..10583
                     /locus_tag="IXT20_000966"
     CDS             10266..10583
                     /locus_tag="IXT20_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAQ4859433.1"
                     /translation="MHIQIPTVLLDGTVLAALSKEDMYGYALTKIVQESLSVSESTLY
                     PVLRRLKKEGYLETYDEPFEGRNRRYYSLTPMGSRHLEEIIDEWIVFRDSLDRLLEES
                     TNE"
     gene            10576..11184
                     /locus_tag="IXT20_000967"
     CDS             10576..11184
                     /locus_tag="IXT20_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HAQ4859434.1"
                     /translation="MNEYLNEVVDHLQEIPEDDRFDLIQYYEEYFLDSGKTLDQIIDE
                     YGTPKQFALKLKISYFSEEEQAENELVKNPKRQIRLIWLIIIGLFASPVLIPLALGFI
                     LVIGGLLVALLALIFSIYVLCISILGVGLFMIISGISVFGQSILSGLFFIGVGLFATG
                     SAVFISPLLLKGTKGLFYVMMNFVKWMGRRFITKRDLHSAGV"
     gene            11200..12030
                     /locus_tag="IXT20_000968"
     CDS             11200..12030
                     /locus_tag="IXT20_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat protein"
                     /protein_id="HAQ4859435.1"
                     /translation="MRLKHFLITGIGLMLTGGLLAGATYTLGAQKSLTWDNGPRLIDM
                     VTETKKIKDANKIVIHAENQTVSVIRGLDFEIKTTYDKTEKPEIKEKNGVLTVNAVKK
                     STGAIISLEDTSAQITIVVPADHELSELTIEGNNSAIDINEVSIKKVNTKTHDGWVSF
                     NEFTGGTITAKDELGSLRLDEANVDNLIVTGENSNFHLEDSLISKEGKVILNGGNLSL
                     HEMENSGYTIQTSGESYIEKDYQPITNTLLEQGQKKLKITAKDTSINITTDQEDNETD
                     "
     gene            12168..12656
                     /locus_tag="IXT20_000969"
     CDS             12168..12656
                     /locus_tag="IXT20_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5348 domain-containing protein"
                     /protein_id="HAQ4859436.1"
                     /translation="MKVEEGQREKLKTEMERLHTYITQLSQTFYDPDKEKVMVNYPNN
                     SEGRQLEQVYHEVFKHLLTVKKELDYYSLPIIDTGILKYDGKKERFIFKSVREDLPLS
                     AGMDLEVLVEDYFTEEKHWVRTKLDYLPQAAGGTQASGWYITEDKELELEGVMARIRK
                     KN"
     gene            12707..13579
                     /locus_tag="IXT20_000970"
     CDS             12707..13579
                     /locus_tag="IXT20_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HAQ4859437.1"
                     /translation="MREKRAFYINGYAGIVGLVVLALVGLFFFYLGMWQAKVWALFLS
                     IFLWLITLLLLSSATVVSPNQAKVILFFGQYLGTIRENGFFLTIPLAQKMTVSLKVRN
                     FNSSVLKVNDLDGNPIEISAVVVFKVVDTAKALFDVAYYQDFVEIQSETAIRHIASQY
                     PYDTFNENDLTLRGNTTAVSDELQKELQERLAVAGVEVIETRLNHLAYATEIASAMLQ
                     RQQAKAILSARQTIVEGAVTITQMALEQIEDGQEINFTDDRKVQLINNLLVSIITDKG
                     TQPVINTGDMKDRK"
     gene            13755..14117
                     /locus_tag="IXT20_000971"
     CDS             13755..14117
                     /locus_tag="IXT20_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HAQ4859438.1"
                     /translation="MKKLLIGLLVFIVVVLAGVKIADYVVMGGEEYYVQITTDGTKTV
                     SKSDSGQETVNYRYILPGYTKEGKEKELDFKGQLTRPLRKGAYLKITWNKNKGVTSYE
                     EVEKKDVPKTALEKLIKG"
     gene            14126..14878
                     /locus_tag="IXT20_000972"
     CDS             14126..14878
                     /locus_tag="IXT20_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ4859439.1"
                     /translation="MNKIVEVQNVTKVYGKNNEKKTQALSGISFDVEKGEFIGIMGAS
                     GSGKSTLLNILSTLDKPTDGHIRINQKDVTTLKGNQLADFRANEIGFIFQDFNLLESL
                     TAQENIAVPLSLQGVRPKEIKQRVQKIAERLSISHILESYPSEISGGQKQRVAAARAL
                     ITQPTILLGDEPTGALDSKSARDLLDTMDELNTKDHVSILLVTHDPFSASYCQRILFI
                     KDGGIHQEVKRGDQSRDSFYREILSILGNLEQ"
     gene            14892..16694
                     /locus_tag="IXT20_000973"
     CDS             14892..16694
                     /locus_tag="IXT20_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ4859440.1"
                     /translation="MLWKLSLTGIKSRLRDYLVLFSGLVMASAIFYMFESMASNEAFL
                     KSNTIVAATVIIFRFGSVLLGIITFVYILYANSFLMTMRQKDYAMFMMLGAKGRKIAQ
                     MIFSETFLVGIAATLVGSAVGVGLTSIVHRLLVDQLDLNISHFTPFSIHGLLITVLFF
                     AVLFLLAAVVNAFSIVKKPILALIRADQTPTRIKQHKFLFFLEVVLGIAFLGCGYYMM
                     KHVGTYQVFGIGVALVTIVLGTYFIFHSVIIFFLSLLKKSEQISLKKLNNFTLSQLNF
                     RIREYTQMLSMVAMLFALALGALTVGLGFKNEIADLTENNSGYDLVMNNPRAEDQQKI
                     KELSPTLSASYTQKEDAGTIYYNASEFAAQPLIMIKHEDGTPPKITKEKVPAKKMNEL
                     TVQEELRSYELPEQQEKEIKVVSQSEFDQLNLPQSTLQLVQVKDFQANLKPIETLVAQ
                     NKMNNPSLQKVEYSNQKYEMYEVYNGLFSGFEFMGFFLGLAFLTMLASCLMFKILSGS
                     KSDIARYEMLEKIGTRRGLLKQSIRREVGVLFLAPGILGVVHVLFGLQMFGLFMQNPY
                     KDIWVPFTIFFVLYFIYYVLTTWLYTGIVLKKRL"
     gene            16971..18011
                     /locus_tag="IXT20_000974"
     CDS             16971..18011
                     /locus_tag="IXT20_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HAQ4859441.1"
                     /translation="MEINEQNFGENYHLITITNQNGTSLSVSDLGARVVHFQTIIDNE
                     KRELILGFDEAKEYLEKDPYIGASIGRMAGRITNGTFKLDDETFEVKTDPDTGHCLHG
                     GAPSFEAKQWHYTVINGENEASVIFYQTSPDMENGFPGTLDVEVRYTLTNDDTWRVTV
                     QGISDRKTVFNPTNHVYFNLTGDPAQSIDEHILWLNSRLFATLNKDTTPTGEVVSVSG
                     TAFDFQRPKKLGEVFASDFRQSMIVGGIDHPFLLKKQSGLLNAAELTSPDEKVSIQVK
                     TDASGIVFFTANFGEDGPEMRGNKLVNHGGITFETQELPGAERLESFGDVTLEPNQVR
                     EFVTEYKINIKK"
     gene            complement(18063..20207)
                     /locus_tag="IXT20_000975"
     CDS             complement(18063..20207)
                     /locus_tag="IXT20_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAQ4859442.1"
                     /translation="MKNKKTIRLLAATWLIAPSVLSTNTVFAVDNQIADQTVTTDTTT
                     SSSATSETTDSFTEDPTGTTNSTTGSTEPTEIDPADPSENEKPAETYSLTIAPSLIGK
                     IQLTASTADSPEQQPLDITESGTVEGLLAETKVTYTITPAEGEQIASVAVNGAKEVNY
                     DAKTFTVGTNNLSITAEFKAGPDTSTEKPTDPSEEVPSTPDPETPNKPDVGNNNNNNG
                     SNNSNNNNNNSNSGNSSNQTNPSGSNNNGNTQREPNHPNNGSSNGGTNNASSRPNTAN
                     QSGIENPSSASSDFVVKSPIDAVLPTNTTNVQQAIVREAFKHLGKPYVWGAKGPNTFD
                     CSGLTYYVYMKATGHYIGGWTGEQQYAGTQIPVSQAQPGDLVFWGPSSGVTHHVGIYI
                     GNGQFIHAPQPGDKVRVTSISDFTPDFAVRVNLAGLPAASGSLTGGSSILDGLDGSFH
                     FSQNQTTDQFLKKIADDAQEIGQKEGIYASVMMAQAILESGSGNSLLSSEPNHNLFGI
                     KGSYKGSSVTFNTLEQDSSGQSYQIRAQFRKYPSYKESLEDYADLIKNGLTGNPDFYK
                     PTWKSETKDYKEATKYLEGRYATDRQYSQKLNAIIEAYDLTKYDEPKKEAATTEEANA
                     EILNMSKRFDVPIKWTTDGVPITGLEFSTIKRVNNASSIFRVASIWDLWNNFTARQIP
                     ETTTRTVKAATVPVLTLLSLDKSFWLDLPLKK"
     gene            complement(20564..21937)
                     /locus_tag="IXT20_000976"
     CDS             complement(20564..21937)
                     /locus_tag="IXT20_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ4859443.1"
                     /translation="METTMNPEQMVLSEQIHMLIEQLGGKDNIDSVLPCATRLRIKLK
                     KQPVANAEKLKQQIPSIKYFILLDRYAEIIMDQGLAPAYKELTRQIDHSAPKEKLNPI
                     EKLFGLISEIFTPIIPLFAGSGILKGLVVLATTIGWLSETSGTYHILSAASNAVFYFL
                     PIFLAFSAAKTFKVNGYIAAVIAAALIEPNLTSLLSDSGKAATDFLGIPVVLMQYTST
                     VMPAILGIFFYSFVEKFLKKYIRENMQLVFVPLLSLVITVPLTLMVFGPAGVYLGEGL
                     AAAITWMMDINGPISGAIIAGGWNILVIFGIQWAVNPVMISNISAYGFDRIVPLTGAA
                     NFGMAGAALGVFLRSKRSKTRSISGSAFASILLAGVTEPTVYGIAIPLKKPFVAACIG
                     AAAGGAVMGFAQVKAIAFVFGSLTTLPAFISGTFFWYLAGLAVSLVVAMITTLVSGFD
                     EDLMSYE"
     gene            complement(21937..22332)
                     /gene="rbsD"
                     /locus_tag="IXT20_000977"
     CDS             complement(21937..22332)
                     /gene="rbsD"
                     /locus_tag="IXT20_000977"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002829567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HAQ4859444.1"
                     /translation="MKKGKVINSDISRVIAQMGHFDKLSIGDAGMPVPFGTEKIDLAV
                     SNGLPSFLSVLENVLEELEVQSIELAEEIKTMNPEILEKIKLLLPDTPISFIPHEEMK
                     KELNNCHAFIRTGEMTPYANIILVSGVVF"
     gene            complement(22360..23277)
                     /gene="rbsK"
                     /locus_tag="IXT20_000978"
     CDS             complement(22360..23277)
                     /gene="rbsK"
                     /locus_tag="IXT20_000978"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HAQ4859445.1"
                     /translation="MNTVTVIGSINMDTTLRLTNMPKPGETMHAHEIFHAGGGKGANQ
                     AVAAKRSGARTSFIGGVGADSEGQQLLDLLTKENIDTSGIAEIQGATTGQAMIMVDAA
                     GENSILIHAGANNAFHEQEVLKNKQLITNSDFIIAQFESSLDATILAFSIAKDAGKTT
                     ILNPAPARETIPTELLEKTDIIIPNETETEIITGIRVTDHNSLVAAAEKLHELGIGTV
                     IITLGSAGAFYHTEKEHGIVPAFKVDAVDTTAAGDTFIGALSSTLQPDLSNLKEAILY
                     GNLASSVAVQSYGAQPSIPYREALEQSAV"
     gene            complement(23385..24398)
                     /locus_tag="IXT20_000979"
     CDS             complement(23385..24398)
                     /locus_tag="IXT20_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAQ4859446.1"
                     /translation="MVKKKITIKEVAKLSGVSITTVSLILNGNTEKFSPKTVQKVLAA
                     KEELGYQPDYFARQMITKETKTIGVLVPDITNPFFSTLMRGIEDILYKQNFVTILCNA
                     DSDHQKEIEYLAELTRRGVDGFIIATSAVSTDAINENLKKQGRPFIVLDQKKSEGFSD
                     AVRTDDFRGGYLAGMHLLSLGHQTIALVYPENPPENVHARIEGFKSALDVYQIPHDQL
                     ILLPTQFSKQGGYQITAELLDSAATGVFALNDELAFGLYRGLEEAGKSIPEDYSIIGY
                     DNIDMCEYIKPKLTTIAQPIFELGQTSAKLLLDRIQFPEKEWEEKRLPVRLEKRFSTA
                     PLK"
     gene            complement(24479..25078)
                     /locus_tag="IXT20_000980"
     CDS             complement(24479..25078)
                     /locus_tag="IXT20_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAQ4859447.1"
                     /translation="MNDLKENMIFDLSKTAFVAIDMQKEIVNNGTLSPHTAASILTNN
                     DLLVRTLKRTAALKVLVNVDISTFPYLSQQTDMGGMTASVPPEFTDLLLKDSLKDTDN
                     MLTITKYNPSAFFGTSLDLQLRRRGIETIILSGVATTNGVYATALDAFQHGYHIVLAE
                     DACSDRDKESHQLFIKKIFPKTARVRSTKQIIEAIQQSK"
     gene            25264..25965
                     /locus_tag="IXT20_000981"
     CDS             25264..25965
                     /locus_tag="IXT20_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ4859448.1"
                     /translation="MKYVEVKNEYKRYKMGETTITANDDISFSIEKGELVIILGPSGA
                     GKSTILNILGGMDTPDEGQIIIDDTDIAQFSDKQLTAYRRTDVGFVFQFYNLVPNLTA
                     KENVELATEVSPDALDPVEVLRQVGLAHRLNNFPSQLSGGEQQRVSIARALAKNPKLL
                     LCDEPTGALDFETGKQVLKLLQNASRQHGNTVLIITHNSALAPIADRVIHINDAKVRS
                     VELNDHPSSIDEIVW"
     gene            25975..28686
                     /locus_tag="IXT20_000982"
     CDS             25975..28686
                     /locus_tag="IXT20_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HAQ4859449.1"
                     /translation="MKTRTYLKAGFREIRQSKGRFIAIILIVMLGTLLFVGVKTAGPV
                     MQKTMDHYVGTAGLSDLQIVSTGGLTQKDIAQAEKIPDAKVETGKQIYYSNTGKNEVI
                     QIFSYNKNNKQNKLQLTDGKLPEKPNQLVLDEKAEDEGYKIGDIYQIDSDELKEKEYT
                     ITGFVRSPLFINNLERGYANVGNGSVDYFVYLPESVFKTDIYSVLYLDFTNVKNLNTY
                     SKAYKDKMEQNQEKAEKYLQDRPEERLEELKKSANEELEPAQQKIADGKAQTTQARTR
                     LEDAKKQLAQQSATIQQLPEAQRTIAVQTLSQQEAQLAEQERQLVEKERELASAEEEL
                     NRNAEKIENLTKPSYLYNERSENVGFQEFGDLSDRIAAIANVFPVFFFFIAALITFTT
                     MTRMVEENRREIGTLKALGYTKVEIAKKYAIYASLASGIGIVLGTILGTNLLPRIIFE
                     LSNERYDIGSALIFYDWPPILQAAAAFFIAAFGAAMIVLFKDLRERPAALLQPKAPKP
                     GKRIFLEYITPLWSRLSFNQKISYRNLFRYKSRMLMAIIGIAGCAGLMVAGVGLKDSL
                     SSVSAKQFGPIIDYQAIVTLDSSTEDSRQKAADMLQKQEKVNEILAIHTQTVELRKKG
                     HASQSLTVIVPSKTADLDRFIHLTDLEENPRSLPKTGIAITQKVAELFDLSTGDHISL
                     YDEDQKEWKVEVKEIVKNYLGSFIYMSPEYYASVTDQSMMDNAFLLRTDKMTTKEEEM
                     LSEKLLATDAVTNTSFMSKQIETQEESMANLDSVVLIFVVLSGLLAFIVLYNLTNINI
                     SERVRELSTIKVLGFFDKEVTMYIVRENIIFTLLGIIGGFGVGYILTDFILQQASMEN
                     VIFPLVITWWAYVLSAGLTIVFTVIVMIVTHFKLKHIDMIDALKSNE"
     gene            29137..30765
                     /gene="treC"
                     /locus_tag="IXT20_000983"
     CDS             29137..30765
                     /gene="treC"
                     /locus_tag="IXT20_000983"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HAQ4859450.1"
                     /translation="MTFKDKVIYQIYPKSYKDSNGDGIGDLKGIKEKLPYLNDLGIDM
                     IWLNPIYPSPQKDNGYDISDYTAIDPIFGTMEEFEQLIAEAKKYRIEIMLDMVLNHVS
                     TEHEWFKKALAGDQHYQDFFILREEPTDWVSKFGGSAWSPFGDTGKYYLHLYDRTQAD
                     LNWRNQAVREELFKVVRFWMEKGVKGFRFDVINVIGKDEQLKNNKENDGKPEYTDKPI
                     THTYLKELNQATFGTDSTIITVGEMSSTTIENCILYTEPSREELSMVFNFHHLKVDYE
                     NGNKWSTPPFDFAELKRLFHTWGEKMSEGNGWNALFFNNHDQPRALNRFVAIDQYRKQ
                     GAEMLATMIHLNRGTPYIYMGEEIGMIDPDYSSISDYIDVESLNAYKLLLEQGFTEKE
                     AFRRIKAKSRDNSRTPMQWSDTEQAGFTTGKPWLKVGGKLEEINVEKELSSEDSILSY
                     YKKLIRLRKTYPIVAQGDYHAYEANHPEVYGFLRQFEGQQLLVLTNFYDKDTRIAIPE
                     GFTEANILIDNYSDTVLHQSLELKPYQAIALLKN"
     gene            complement(30825..32006)
                     /locus_tag="IXT20_000984"
     CDS             complement(30825..32006)
                     /locus_tag="IXT20_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAQ4859451.1"
                     /translation="MKKETDTTNRLIRFLGGKTSYYVLGIVILLALIIYFFHQISFIF
                     KPVGIIIATILPPLLFALILYYLLEPVIKRLSKHLSRNLSVIIVYVIILFLLGLGSIW
                     LIPFLEEQAKTLISSLPDLFQDFQESLRQFLEKTPFADSFNQFFASIDDVTSDIAGFI
                     GNYWESGAQRISNIFSTVTAIFITLFTGPIVAFFLLRDPQKFYQSVLAIVPPAFRTDF
                     KNLTKIANQQLGSFLKGQIIASFILGAVYWVCFLLIGLEFASVLAISAGLLCIIPYIG
                     PFIAFFPGLIIAFQDSTFMAVKFVIVWFAVQLLHGDLVIPRVMGDRLQIHPITILIVL
                     LVMGDLMGIVGVIFGIPIYTLVKLLVTFIFRKFKQRYNKFYGDKGEYENSSFSEEDYF
                     K"
     gene            32258..33781
                     /locus_tag="IXT20_000985"
     CDS             32258..33781
                     /locus_tag="IXT20_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HAQ4859452.1"
                     /translation="MKRLIGILAMLVLAGMLTACGASGKAEDSKEKLSVMTTFYPMYD
                     FTKAIVGDEGEVELLIPAGTDSHDYEPSAKDMAKIQDTDIFVYNDENMETWVPAIQKT
                     LQEGNVHTIKATEGMLLLPGSEEGHDHDHEHGEEGHTHELDPHVWLAPSLAIKQVANI
                     RDQLIEAYPEKQEVWTKNAAAYTEKLQALHQLYQETFKQAKQRSFVTQHAAFNYLALE
                     YGLNQVSIAGLSSSEEPSAARIAELKHFVKEHGINYIYFEENAKDSIARTLANEAGVS
                     LEVLNPLEGLTNEQIENGENYLSIMEANLEALKKTTETENPLEDTLTPKKEKSVYNGY
                     FEDEDVQDRSLSDWAGTWQTVDTYVEDGTFDPVFEYKEKLNQDKTAQEYKEYYTKGYQ
                     TDIASIKISEKEMAFTFKNGTTKRSNYRYVGKEILTYEAGNRGVRYLFEAEDKESGAF
                     RYIQFSDHTIAPADSEHFHLYAGNESQEVLLKEMENWPTFYPDTMDGREIAQEMLLH"
     gene            complement(33850..34578)
                     /locus_tag="IXT20_000986"
     CDS             complement(33850..34578)
                     /locus_tag="IXT20_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ4859453.1"
                     /translation="MIPIYICEDDPKQLEVLSTVIKNRIMIENLDSYVHISTSDPEEL
                     LNAAKIRTSSFGIYFLDIELEDSEMDGVAIGKLIRELDPLGKIIYVTSHTEMSMKILK
                     SNIEPTDYIVKEDLYDLKENVEHILSKIFEDFQMSQIQKDIFKIEFNDEIKFLPIKDI
                     QYFSTSLGAPHKLEVHLTHAQLQFYEKIKEIEKMHRNFIRCHKSYVINTQNVRSINKK
                     TREVTLANGETIPASIRGLKKLIS"
     gene            complement(34591..35871)
                     /locus_tag="IXT20_000987"
     CDS             complement(34591..35871)
                     /locus_tag="IXT20_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HAQ4859454.1"
                     /translation="MFLWSIALQYLIYTICILLIDHHIRYKYLFAGVILISCILIGWV
                     YSILGIFTALIAIGMLMLTFFFFSTSRQQLVYALPFGLLTSLLGDHLTSIGDFFVLKS
                     SVIEPGDSLQYFHIVLSAFLSALLAFCLKKSLETWFHTVDRRMIGTIGTLILFTYYII
                     IFYTRFSGETPDVLALNTSFFILYLCVGLIILVYYWKISNKKLADQLLEQQVRMQQDY
                     IKDLEKNYQDLREFKHDYQNLLFSLHSYISEGDLEGLRSYYDQTILPTRQMMNLFPAN
                     LSMLDNMKVPEIRSLLSLKLMMAQEKGLIVQLHFPEPIELDTKHTVNLVRMLGIILDN
                     AIEGASAAKKKKIELTILKKNHSVMIRVINTTTNTLPLNQLKQKGFSTKTNPKNEGLG
                     LFILDELTKTNPYLFLETSIENQRFSQTIYIQIK"
     gene            complement(36012..36614)
                     /locus_tag="IXT20_000988"
     CDS             complement(36012..36614)
                     /locus_tag="IXT20_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory regulator AgrB"
                     /protein_id="HAQ4859455.1"
                     /translation="MKDKVVNKLLEALFSGDKSEEDMVYVQVKFVLEVLLNNLGKLAV
                     VLVFSLVTGSWAETGITFLSYICIRRYAYGLHSDSEFVCLLWTLLYLWGVPLVMKHLQ
                     LTISFPMMVFLLISCFLLLLRYGSRGTAINPIEPEKRPPLLKKAISMFLIFSLITLFF
                     SASYFSTYLLLGIVLEIATLLPITNYLMNFGGIFHEKNNH"
     gene            36859..37065
                     /locus_tag="IXT20_000989"
     CDS             36859..37065
                     /locus_tag="IXT20_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4859456.1"
                     /translation="MENEQLFEVGETYAFKSDLFESPIKGKIEKIYENSVMIRVMKCA
                     VEDNDTAHNLHWQLVVRKSSTIEE"
     gene            37377..38096
                     /locus_tag="IXT20_000990"
     CDS             37377..38096
                     /locus_tag="IXT20_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HAQ4859457.1"
                     /translation="MPIIVIIVAGTIGAGKSTLTEMLAQDLETKPFYENVEDNEVLPL
                     FYSNPEKYTFLLQIFFLNKRFLAIKDAFSHDDNVLDRSIYEDSMLFHLNADLGRVSEV
                     EVKQYEGLLETMLKELEEISPQKKPDLLVYIRVSFETMLARIKKRGREYEQLEQDPEL
                     YSYYKELNRRYEEWYEQFDICPKIVIDGDKYDFVADPACGEQIVQEIKMRAKKMTEEA
                     DAQSIRYTTNNPKAKGIFTTK"
     gene            complement(38177..38860)
                     /locus_tag="IXT20_000991"
     CDS             complement(38177..38860)
                     /locus_tag="IXT20_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1275 domain-containing protein"
                     /protein_id="HAQ4859458.1"
                     /translation="MQTSFHEGRTIGLLLTFIGGAMDSYTYIQYGAFASAQTGNIILS
                     IIQAFDGEWLSVGKKVLSTLFFFLGILLTKYLIDYFRKKEKHFWRLFVLYYEAVIFFF
                     VSLTPVHEHPALVTILIAFTASIQWVAFDKINGRAYTNLFTTGNLKGVATNLYDYCIS
                     KEKADFDRFFHYLSVVLAFIAGAIVLVFCHHLFGAHSILIVSILFLIMALTQTFHLWQ
                     FYHTNRFTI"
     gene            39078..41264
                     /locus_tag="IXT20_000992"
     CDS             39078..41264
                     /locus_tag="IXT20_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HAQ4859459.1"
                     /translation="MNEKEILRLQKELKRWENKYKKLQQVFRLQEEIIATYEAAYGEA
                     PGLIEEEVQVKESEGNSSFFKMMEKRSYNRFNQEQKAAITYDMKKHLRIIAGAGSGKT
                     QTICAKAVYLMTQKQVDEERILMITFTRNAANELKKRVDNFSQRKTAVHIGTFHSIFF
                     RIYNEICRKFPEVAMQGIQGDFSKDTAQKVNAVLQQLIRKYNLYIFDQYGEKTIASRL
                     DYWQNMNLSVEEMVQLVKEKYDSIDKNARQPISERLYGLLTELQEQKRSQQLLEFNDV
                     LQNLKSALENEEIQHYIGQKYDYLFIDEFQDTNPLQWEIVKRMTKENRIKLIVVGDDD
                     QSIYGFRGSEPAYIKNFEKEYPTKTLFLLTNYRSRAAIVQGANRLISYNKNDRIPKAM
                     IPAQKEAGVMEAYLFSDTKAESQWLIKQIQTFIQKNGKYKESIILYRSASQTQQLVQS
                     LLKTNIPFVLEADSPYEGIFGIKSFQHFYQKIVHWQTALNLQKKNQAYQQLLRQLMAD
                     CYLKKSECDQYFSPKYQNIAVADYILSISPNLKSKSAEIREFEEALRKTDQHQEIYSL
                     VQSYLHLPRIAKEIDQSDQEWIREEVKQHATFRRLQALSQETQATAKELKTRLIAYRQ
                     GKLDALCIQSIHKSKGLSYRNVFLIGCNEGSLPYNGATEKKVIDFNEIKAEPVTTIEE
                     ERRLFYVAMTRARNRLYLCVPQMKNSRKLKVSRFIKETGSRVKKGK"
     gene            41610..42020
                     /locus_tag="IXT20_000993"
     CDS             41610..42020
                     /locus_tag="IXT20_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HAQ4859460.1"
                     /translation="MIKVYTSPSCTSCRKARAWLQANQLEFEEKNIFAEPLTESEIKK
                     ILAATEGGVQDIISTRSKIYEKLGIDFNELTLKQMIVLFKEYPSLLRRPILIDEKRIL
                     IGFNEDEIRSFVPREIRCVARNLIYLQNNNILNS"
     gene            42034..42390
                     /locus_tag="IXT20_000994"
     CDS             42034..42390
                     /locus_tag="IXT20_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase"
                     /protein_id="HAQ4859461.1"
                     /translation="MEVKVYGSAHCVTSLKAKQWLEHHQLPYQFVDLEERDLSTEEAE
                     ELCSFEEVQAEQLFATWSEAFHNIAIDLSCVGKKQLIFLCTQHTNLLRRPLIIIDEVL
                     FIGYNEQLMEQLILNK"
BASE COUNT        13980 a         7546 c         8575 g        12794 t
ORIGIN      
        1 aggaagaggt tgtgagcctg ccgtttaact ctgagtcaca aggagcaatt tcaaaaaaca
       61 gcttctcatg tttgttgact attgtgactt gtgaccgagg agttggcagg agaacaccgt
      121 ttattcgaaa aaaggaactg cgacaagact tttgtcacag ttccttcttt tttttgctat
      181 gctatactag atgtaaaaaa taggagaaat aaaatgaggg aaatttattc ttttgaatca
      241 aaaatctatg cttccgaagt cggcaagggc ggggcatata ttatttttcc gtatgatata
      301 agaaaagaat tcggtgctgg aagagttaaa gtccaagcaa cttttgacaa tgtgccgtat
      361 gaaggcagta tcgtgaatat gggcgtgaaa aatccagatg gttcagtctg ctacatcctt
      421 ggactacgaa aagatattcg taaaaacatc ggaaaagaaa tcggtgatat tgtagcagtt
      481 acagtgaagc aaaaataaaa gaggttgtga caaaagacgt gtcacaacct cttgataaac
      541 ggtgttcaaa agccagtttc tcggtagtaa gtcaaattta tgcaaaaatg tggaaaccca
      601 tgtttacaaa atttttctta ctactcgaaa cgaagcggct ttatcacaac ctcttgataa
      661 acggcgttca atcagctgtc cctcggtatt agctcaaaaa cggcaaaata tgagaagcta
      721 tttttccatt tttttctctc atactcaggt cataacgctg atttcacaac ctcttctttc
      781 ataatggttt ttcaccatct ttataataaa ctggttcttt gatatggaaa tctatttttt
      841 ccaaccaatc taaaatcttg taacctaact cttctgacat cggaaaagag gcgttgattt
      901 gtgcattgga gatatggtca acatggatac cggaagtgat cgtacaactt cccggaacgg
      961 cagttttgat gatcacagct attttttcgg ctaagacatc gtctttatgg aaccgaccat
     1021 cgtggctagg gaaacgtaca gtttgcatct ctgtatcacg agaaaatgta gtgactgttc
     1081 cgatatgtgg tgtatctccg cctgttaaag tgatcaataa gtctttaccg attactttaa
     1141 cagcagcttg gatcgtgaaa cctagtttgg ttacttcaac tatcgtttct ttttccatat
     1201 taatcaccct gccaacgttt tgaatgctca tttacaattt caaattgatc caaaagtttc
     1261 attgtatggt gattaacaag atcttggatc gtttgaggct gattataaaa agcaggaatt
     1321 ggtggtatga tttgcacacc tagtcgtgct aatttagtca agttttccaa gtgaatggca
     1381 tttaatggcg tttcacgagg aacaatgact aactttcgtt gctccttcat tgttacatca
     1441 gcagcacgtg caatcaggtt gtcgcctaaa ccaacagaga tacctgcgac tgttttcata
     1501 cttgccggta cgatgatcat tccatcggtc aaaaatgatc cgctggcgac agcagcacct
     1561 aaatctttgt ttgaataatg gtaatcaaac aggctttcga attcagttag tgataagtcg
     1621 gattccagct ttaagttctg ttttgcccat ggactgatga ttccgtgtgt ttctatcatc
     1681 ggatattctt ttagtttctt cactaggttg atggcataaa tggtgcctga agcgccactt
     1741 acaccaatca cgattttttt cttccgttgt gccaattgtt ccattttccg cactcctttt
     1801 tatgatgtca aaacagttgc agatcattca ttatttcaaa tatttttccc aatcttctac
     1861 ttcttggaat tttgcacgta caaaatgttc tttcatatcg aaaggaactg taccgtcaaa
     1921 gatagttttt gcacttatcc cacggaaacg aatagagcca ggattatatt ctggtaattc
     1981 tgatgggtcc aatggatggt tcctcattcc tgggatcacg atgatatctt tatctccttg
     2041 gaaacgagtg ttgaaagtcc aaactacatc gttcatgtcg aaaatatcaa catcttcatc
     2101 gactaaaatg actgtcttca attctttgaa ggctgataaa gcaagaatag ctgcttgacg
     2161 ctgaatacct tcatccgctt cgttttcttt acggatttgc atgatggtca tcaatttacc
     2221 accacctgca ggaggattgt agacatttac tacttttcct ggaatagctt tatcgaccaa
     2281 ggcaagaata cttgcttctg taggaatacc ggccatagat acgtgttctt cactgggacc
     2341 aatcgttgtt tgcatgattg gattgtcttt tcggtgagta actgctttga ctttcactac
     2401 ttgtacagct ggattagcat ccccagtata gccaggaaat tctggcattg cttttcctga
     2461 gttcgtgttg atatcttctt gcattgtttc atttggcatg atttctgctt caatgatgaa
     2521 ttcagcacga gcaataccca cttcatcgac tgctatacca tcaacgattt gaataggttc
     2581 gttacgcaaa gctcctgcac accatagttc attgtagcca agtggagtgg taggtggttc
     2641 gaaagtcgtg ccaatggcaa ttgctggatc taaaccaata ttgatagtga taggcattgg
     2701 tttattcaag gcttcgaatt gtttttggaa gtgaccaatg tggcgtccgc caggcatgat
     2761 gtacataccg atcgtatctt tatcttctag aaccatacga tggatcgtta catcgctcat
     2821 tgttttttct ggatctgatc ctaagactac acccattgtg atgtatggac cagcatcttc
     2881 aggagtattt gtaggtgcag gcaaaatgtt gcgaatatcg aagtctgcat cggtagctag
     2941 atgaacgact tcttgtgctg gagcgtctgc tttgtctact ttcacaggtt tgattggatt
     3001 ttctacagcg tctttcaaga aacgaccaag tgttcgataa tcatggtgca aaatttcacc
     3061 tacacgttta cgactagcca ttgttccgat gttcacacgt gtgccgggga aacctttgat
     3121 attgttgaaa gtcatagctg gtccttcttg agtcggacgt tttactgtac cgccagcacc
     3181 gatataacgg taaacaccag ataattctgc ttcaggatcg atttcaatat ctgtttcgtg
     3241 atattgacct ggaagtgttt ttagttcttc gatgactttt cttaaatcat acggttgatc
     3301 tgtcatgaga gattcctcct tagttgtctt tacctatcta tagtaaaagg tgaaaaagag
     3361 gaaaacaatt caacttgttc ggtaaagtat tctacaatgg aatggatgcg tttttttgat
     3421 aatagaataa actatgatat acttatcact aaaagaaagc gcatacttag tgttggagga
     3481 aggaaagtga aaaaaatgga ggagtcattt cctaaagcag taaaagtgga aaatatagcg
     3541 aatattttga aagttacatt tgaaaacgga gaagtaaaat acgtgaaatc tcattggaca
     3601 gaagagataa ccgatgcctt gcaatttgga aaaaaaggaa gaggaaaaag aaaaaatctt
     3661 ctcgcacttt ctcgtaatat gtggattggg actgaagtaa cgattgaagc agatggaaca
     3721 gtattcataa atggaaaaga caggtatacg ccagaagagt tgtggtacaa aggaaagaaa
     3781 agtataccag aattataaaa aataattaaa aaaaacagcg cgaaacaagg aacatattac
     3841 ttcctcgttt cttgctgttt tctactattt cagcgctctt atgactcaaa tctaaatgtg
     3901 gttgtcacaa ccttattctg cacgcaaagc gatagcggcg tcttttttcg ctgccattcg
     3961 tgcaggaaga tgaccgccaa gcatcgtcaa tatagtagag acgatcacta gaataatcgc
     4021 atggacagga tttagttgag caacattttt tagatccgtc atgttgtata agatgctatt
     4081 gatagggaac gtagctagcc atgcaataaa cacacctaat atcccagaag aaatacctaa
     4141 aatacacgtt tctgcgtcaa atacgcgagt gatgtctttt ttacgtgcac ctaaagcttt
     4201 tagtacaccg atttcttttg tccgttcaat cacagatgta taagtaatga tactgatcat
     4261 gatcatactt gttactaaag agatccctgc aaaagcaata aggacataag tgatagcatc
     4321 catcaatccg ccagtcagtt cagtcatcgt tccagctaaa tcagtataaa tgattttatc
     4381 ttcatctgat ttgcctttat tgtaatcatc tagataatcc aagatctttt ctttgtcttc
     4441 aaaattgttc ggataaatca tgatacttga aggcaagctg tctccaccta aataactaag
     4501 caatgtttgt ttggcatttt cgtcaacttt ttcagtagtt aagacattta caccactgtc
     4561 tctttgagct ttgacgattt ctgattcttt gttttcgttg acgatttgag tcgttaattg
     4621 atcgctataa gcgattcctg gagataagag attcattgta gaagaagatt tcacgcgtag
     4681 gatccccgag atcttcaatt catcagaagc attctgatac atcgcatcat aatctgtatt
     4741 agggataaaa ttcccagttg gaagttttgt atagtaagta ttgttgttta ctaatttgaa
     4801 tgttgtacca acgatatcat caaaatcaag tttttcgtct tctttcacat caaatccaag
     4861 attcttcaat gcgttgatat tagtattatt attgccgtct acgatcagca cgacatctgt
     4921 tgctgaggct ggataacttc cggcaagtaa agaataattg tcttttaaaa agttttcttt
     4981 gctagtgtca agctgtgtag ggaaacttga cactcccaca cctgtcatcg ctgacatcgt
     5041 actagataaa gaaagagatt ccgtatcagg attttgattc gagaagctga caggctgtac
     5101 tttgccattt acatcacgta aaagattgat tcctgtagta cgggtaaatc cgatattatt
     5161 acttaagtta ggatcgatgt ctgttacgta atcgacgtat gtttgatcaa tcttattcgt
     5221 gtgctgtgct ttatcttctt cgctgacttt tgcagtaatc gttttgctgt ctggataatc
     5281 cgctttgctt gcacccaatc cggcatcgtc acgagtctgg ttagtcgtta ccttagaaat
     5341 agtaatcgga aatttcgcca tcgtttcagc ttgcgtgtta tcgatttgtt tttggaatcc
     5401 agaagaaagg gacagaacga tcgcgattcc gatgatccca atactggaag caaaggaggt
     5461 caagaaagta cgtccttttt ttgttcggat attgttgaag gaaagcttta acgctgtcca
     5521 aaaactcatc tttgttctgc gtaagttaaa ttgatcatct tttggtcgtt cgatgtgagg
     5581 atgggaatct gttaagatct taccatcaga aaattcaatg attcgatctg cgtattgatg
     5641 ggccagttca ggattatgtg tgaccatgat cactaatttt tctttcgata actcttggat
     5701 caattccatg atctggatac ttgtttctgt atctaacgct ccggttggtt catcgcacaa
     5761 aaggatatct gggtcattgg ctagtgctcg agcgatggcc actcgctgca tttgtccacc
     5821 agataactga tttggctttt tatgcatatg atttgtcaag ccgactcggt gcaatgcatc
     5881 ttctgcccgt tttcgttttt cgtctttaga aacaccactt aatgtcatcc caagttcaac
     5941 gttttcaata atgcctagat gaccaatcaa gttgtaactt tgaaaaatga aaccgataga
     6001 gttgtttcgg taggcatccc agtcactgtc cttaaagtct ttagtagact ttccgttgat
     6061 taccatatca ccggaatcat aattatctag tccaccaatc acattcagca tagtcgtttt
     6121 accagaacca ctaggaccta aaatcgctac gaattctttt tgtctgaaag cgacagacac
     6181 accatccaat gctttggtgg tggtttcgcc gactttataa tattttttta tatcttttaa
     6241 ttgaagcata atcacccttc tcccgtattg aaatattcgg tatactaata agtatatcag
     6301 aacaatgatc gtagtatacg aaataaatgt taactgaaga tgaactacaa gaaaaaatct
     6361 agaaattttt caaaaaacag agggagatca atgaatcgta ttttagtagt agaagatgat
     6421 ccgcaattac agttgcttta ccgttctgtg ttggaacgag cgggcttttc agtcatcacc
     6481 gcattgaatg gagaggatgc gctgaaaaaa ctagaagcga cacgtataga attaatcatt
     6541 acagatatca tgatgccgaa aatggatggc tatgacttgc tagactcatt acgtagcacc
     6601 agatttgaca cacctgttct aatgattacg gcaaaggccg attttgagga taagaaacgc
     6661 gggttcaaat taggagcaga tgattatatg acaaaaccca tcgatgtcaa tgagatgatc
     6721 ctacgtgtgg aagcattgct tcgacgttca aagatcaatt acagcagtag cttgataatc
     6781 ggacaaacaa aattagagct agatgcttat acagtgttac aagatcagca ttcgttgatt
     6841 ctgcctcaaa aagaatttct gcttctgtat aaattactgt cttatcccaa caagatcttc
     6901 actcgccaac agttgatgga tgatatttgg ggattagata cgaatacaga agaacgaacg
     6961 atcgatgtac atatcaaacg tttaaggacc cgttttgaaa agacggaaga ttttcaaatc
     7021 atcacagtac ggggactagg ctataaggcg gttatttaaa gatgaaaaaa aagctatttt
     7081 cacaattatg gatctatttt gctagtattg tcttcatttc gatcttagtg acactgctgt
     7141 gttttctcgg ttttattttc ttattgtctc gacattctat ctcgcctgct gaatcggctg
     7201 gaaaaccaac gttatacccg ttattcgtct ttgcggggtt cagtatggtc gtgggtacag
     7261 gaatatcgat ttttgttggg cggcgtatcc tgcatccaat cagtgcgcta gggacaaata
     7321 tgagtctggt agcaacggga gatttttcga ttcgaatgga tgaacagcag aaagtagcag
     7381 aagtacagca actgtataaa gatttcaacg ttatggtcca agaactcaat agtatcgaga
     7441 cactgaggaa tgactttgtg tccagtgtat cccatgaatt caaaacacca ctagcaacaa
     7501 tccaaggata tgttcaattg ttacaagccc caaatctctc tgacgaagaa cgccaaatat
     7561 ttttatatcg gattatcgaa agtatcacgc agctttcaca attaacagag aacatcttaa
     7621 aattgaacaa actggaaaac cagcgtatcc aactggaaaa gaaagagtat cgattagatg
     7681 aacaaatcag agaagtaatc gttttttttc agcctaaatg ggagaaagaa cagctagaat
     7741 tggatatcga acttgctgca gtaaattaca caggcaatga agagtttttg taccaagtgt
     7801 ggctgaatat catggataac gccataaagt acaatcaaat caatggacag attcatatca
     7861 aactatttga gacagccaca gagattgtac tggaagtgac cgattcaggt gtaggaatga
     7921 atgaagagac cagagatcga atgtttgaaa aattttatca aggagataca agtcgtcaaa
     7981 tttctgggaa tgggctagga ctttcactgg ttaaaaaaat tctagaactg catgatggac
     8041 gtattgatta tagcagtatc gaaggagtag gaacgacagc aatgatacgg ttaagtaaac
     8101 aataggaaaa agaataaaac ccctaaaaat tctattgaag gtttttaggg gtttttagtc
     8161 gtttttttgt gattagggaa atgttcttac ggttcttttg gcataaaaat cgctgctttc
     8221 ttattgaact acgagaaatg ttagaggtgt cattgaaaca aggacatgat gcaagaaaga
     8281 gagcgaaagt catgcgtcgc tctctttttt tgggtggata gatggacaga aaatgtaggg
     8341 cttagttttc ttcttgattt aaatactccc aaaatgtatc tgaagccaaa gaatgctcac
     8401 cgactttacg agtaaatgca agataactct ctgttgttgg agaaataggg agaatcacag
     8461 tattttcttt taatagattc ttgactgttt tttccataac aatagcaatc cccatgtcat
     8521 ttgcaatcat attgatgatc aagtcgattc ttgctccttt ataagagatt tttggttcaa
     8581 atccagcttc gcgacacaga tctagtacag gttgatacaa gttcgtagtg gggccaagga
     8641 gcaaaaagtt ctcctttttc agttcgctta gatccaattt ctttttacta gctaaaggat
     8701 gatttttagg tagaactgct acaaaatcgt ctgtttcagt aggcaaccac tcaatcgaac
     8761 ttggtggggc ctggaaactt ctagcaaagt ggatcgtgtc actatgttca ttggcgtcat
     8821 tgtaagttaa ttcgacactt tccacttctt tcaactggac tgtaaattca ggatggcgtt
     8881 tcaaaaactc ggtgattttg gaaaaacctt tataattgat cattgtcggt atcgttaaga
     8941 tatttaatga aagattgttg tctttcgtat aggtttcaag cacttcttgc atagcatggt
     9001 attgttctac gacatttttt acatatggga gcaataagct tccagcttcg gtcaattcga
     9061 tttttctatg ggcgcgttcg aataatttga cgtctaattc tgtttctaac gaaagaatct
     9121 gtttagaaat atttccttgg gtggtgaatt gtcgatcagc agttttggtg aaattcattg
     9181 tttcggctaa atcaagaaaa acttcgaatt tatggatatc catcatattc ctcctactta
     9241 ttccaataaa gaatacttta acacaaaaaa tgaattggtg taatcttctc tgtctgttac
     9301 aatctttctt gcagcgaaag caataattac agaaattttt ctgatttttt tcgcttcgta
     9361 gaaaaagaga ggagtttttt gatgccaact ggagttttga tcaatgtagg ttccgttttg
     9421 tttggcggac taattggcgg attagtagga aataaattaa gtgaacattt taaagcacag
     9481 ctgacgatgg tttttggtgt gtgttcgatg gggatgggaa tctattccat tgcgccgatg
     9541 aaaaatatgc cggctgttat ttttgcttta gtgataggaa cagcgattgg tttgattgtt
     9601 catttaggta atgggataaa taagggggct gcgctgatgc aagtacctat ttctaaaatc
     9661 ttccctagtg aaaagcttgg tatgacccac gatgaattta tcagcacatt agtgacggtc
     9721 attgttctat tttgtgcaag tggtacgggg atctatggaa gtttagattc tgggatgaca
     9781 ggagacagta cgattttgat ttctaaatct gtccttgatt ttttcacagc agctattttt
     9841 gcgtgtaatt tagggtatgt cgtttctgtg atagcaattc cccaattcat tattttttat
     9901 attttgttct tattggcaaa atttatttat ccattgacta caccagacat gatcttagat
     9961 tttaaagctt gcggcggatt cttgatggtc gctaccggtt ttagaatgat caacgtgaaa
    10021 atgttcccag tagctgacat gatacctgca atgatcgtta tcatgccact gagctggatg
    10081 tggacaaatt ggattatgcc actgttataa taaaaatcaa agagaagcca ttttttgctg
    10141 actaaaaagg atcaaatctt ttagtcagtt tttttgtatt tgacaaactg attttgaaac
    10201 tatattatat gttgtataat atagattgaa ataaaatatt acacttgaga atagaaggtg
    10261 acgtaatgca catccagatt ccaactgttc ttcttgatgg tactgtgcta gcagcattaa
    10321 gcaaggaaga tatgtatggg tatgccttaa caaaaatcgt acaagaaagt ctctctgtca
    10381 gtgaatctac actatatccg gttttgagaa gattgaaaaa agaaggatat cttgagacct
    10441 atgatgaacc ttttgaaggc agaaaccgtc gatattactc gttgacacca atgggaagtc
    10501 gtcatttaga agaaattatt gatgaatgga ttgtttttcg agattcttta gaccggttat
    10561 tggaggagag cacaaatgaa tgaatatttg aatgaagttg tggatcatct tcaagaaatt
    10621 cctgaagacg atcgttttga tttgatccag tattatgagg aatatttttt agattctgga
    10681 aaaacattag atcagatcat tgatgaatat ggtacaccaa agcagtttgc cttaaaatta
    10741 aaaatcagtt acttttcaga agaagaacaa gcagaaaacg agttagtgaa aaatccaaaa
    10801 agacaaattc gcctgatttg gttaatcatt atcggtttat ttgcttctcc tgtcctcatt
    10861 ccactagcac tggggtttat attggttatt gggggactat tggttgcttt actagcgctc
    10921 atttttagta tctatgtttt atgtatcagt atcttaggtg ttggcctatt catgatcatc
    10981 tcaggaatca gtgtgtttgg acaatcgatt ctgagtggat tattctttat cggtgtcggt
    11041 ctgttcgcca caggaagcgc agtatttatt tcgccactgt tgctgaaagg aacaaaaggg
    11101 cttttttatg tcatgatgaa ctttgttaaa tggatgggac gacgttttat tacaaaaaga
    11161 gatctgcatt cagcgggcgt ttgaaggagg acacataaat tgagattgaa acattttttg
    11221 attaccggta ttggtctgat gcttactggt ggcctcctag caggtgcaac ttatacatta
    11281 ggcgctcaaa agagtctgac ttgggataat ggtcctcggt tgatcgatat ggttactgag
    11341 acaaaaaaaa tcaaggatgc aaacaaaatc gtcatccatg cagaaaatca aacagtttcg
    11401 gtcatacgcg gattggattt tgagataaaa acgacttatg ataagacaga aaagccagaa
    11461 ataaaggaga aaaatggcgt gttgactgtg aatgcagtca aaaaatcaac aggtgcaatc
    11521 attagtttag aagacacaag tgcacaaatc actattgttg tccctgcaga tcatgaatta
    11581 tctgaattga caatcgaagg gaataatagt gcaattgata tcaacgaagt atcaatcaaa
    11641 aaagtgaata ctaagacaca tgatggctgg gtatcgttca atgaatttac aggaggaaca
    11701 ataactgcga aagatgaatt aggctcactt cgattagatg aggccaatgt ggataacttg
    11761 atcgtcacag gtgaaaacag caacttccat ctagaagaca gtttgatcag caaagaagga
    11821 aaagtcatcc taaacggtgg caatttatct ttgcatgaga tggaaaacag tggctatact
    11881 atccaaacgt ctggtgaatc ttatattgaa aaagactacc agccaataac aaatactttg
    11941 ttagaacaag gacagaaaaa gctaaagatc actgctaaag acacatcgat caatattacg
    12001 acggatcaag aggacaatga aacagattga tcctgtactc aactggatat tttaagaaaa
    12061 aacagcaaag atccttccaa tctttgctgt ttttcatcgc ttaacgcaca taactatagt
    12121 aaaataagaa agaaaatatc taaaaagaaa acagggtgaa agagtatatg aaggtagaag
    12181 aaggtcaaag agaaaagtta aaaacagaaa tggaaagatt acatacttat atcactcaac
    12241 taagccaaac cttctatgat ccagacaaag aaaaagtgat ggttaattac ccgaacaatt
    12301 cagaaggtcg ccaattagag caagtgtacc atgaagtttt caaacattta ttgacggtga
    12361 agaaagagct ggattattat tctttaccga ttatcgatac aggaatattg aagtatgatg
    12421 ggaagaagga acgatttatc tttaaatctg ttcgggaaga tttgcctctt tcagcaggga
    12481 tggatctaga ggtcttggta gaagactatt ttacagaaga aaagcactgg gttcgaacaa
    12541 aattggatta tttaccgcag gcagccggag ggacacaggc aagcggttgg tacatcactg
    12601 aagacaaaga gttagaacta gaaggtgtaa tggcgcgtat tcgaaaaaaa aattaaaaaa
    12661 gtaacattag atatttattg aaggataaag gagcgcagga agtagcatga gagaaaaaag
    12721 agcattttat atcaatggtt atgcagggat tgtcggattg gtcgtattag ctttagttgg
    12781 attgtttttc ttctatttag gtatgtggca agcgaaagtt tgggcacttt ttttaagtat
    12841 ttttctttgg ttgatcacgt tattgttgtt aagttcggca actgtagtca gtcctaatca
    12901 agcaaaagta attttgtttt ttggacaata tttaggtacg attcgagaaa atggcttttt
    12961 ccttacgata cctttggcac aaaaaatgac tgtttcgttg aaagttcgga atttcaatag
    13021 ttctgtttta aaagtcaatg atttggatgg aaaccccatc gaaatctcag cagtagtagt
    13081 ctttaaagtc gttgatacag caaaggcatt attcgatgta gcgtattatc aagattttgt
    13141 agaaatccaa agtgagacag ccattcggca tatcgctagc caatacccgt atgatacatt
    13201 caatgagaat gatttgacgc ttcgaggaaa cacgacggca gtgtctgacg agttgcaaaa
    13261 agagctgcaa gagcgtttag cagtagcagg tgtcgaagta atcgaaacac gcttgaacca
    13321 tttagcctat gcaacagaaa tcgctagtgc catgctccaa cgtcaacaag caaaagccat
    13381 tttgtctgct cgacaaacca ttgttgaagg agctgtcaca atcactcaaa tggccttaga
    13441 gcaaatcgaa gatggacaag aaatcaattt caccgatgat cggaaagtcc aattgatcaa
    13501 taatttactt gtctcaatca ttacagacaa aggaacgcaa cctgttatca atacaggcga
    13561 catgaaagac cgtaagtgac atactttttg taagatagga agaaggagtt taggacggat
    13621 tattgtcttg aactcttttt ttagttgttt taaagaaaat gacaaaattg aaagaagaat
    13681 gatagttgat tcgaaggcag aattgttcgc ttttttctat aatcagtata taaagtaaga
    13741 gagaagggga aaaagtgaaa aaacttctga tcggtctact tgttttcatc gttgtggtac
    13801 ttgcaggggt gaaaatcgca gattacgtag ttatgggtgg agaagaatac tatgtacaaa
    13861 ttacgacaga tggtacaaaa acagtttcaa aatcagactc tggacaagaa acagtcaact
    13921 atagatatat cctgcctggc tatacgaaag agggtaagga aaaagaactg gacttcaagg
    13981 gacaattgac tcgaccgctt cgtaaaggag catatttaaa aatcacctgg aacaaaaata
    14041 aaggtgtcac ttcgtatgaa gaagtcgaaa aaaaagacgt gccgaaaaca gcactggaaa
    14101 aactaattaa agggtaggga aagaaatgaa caaaatagtc gaagtacaga atgtaacaaa
    14161 agtatacgga aaaaacaatg agaaaaagac gcaagcatta agcggcatca gcttcgatgt
    14221 agaaaaaggc gaatttattg ggatcatggg cgcttctggt tctggtaagt cgacactttt
    14281 gaatatttta tccacattag ataaaccaac agatgggcat atccgaatca atcaaaaaga
    14341 tgtgaccaca ttaaaaggaa atcaactagc agattttcgt gcgaatgaga ttggctttat
    14401 tttccaagat ttcaatttat tagaaagctt gacagcacaa gaaaatatcg ctgttcctct
    14461 ttctttacaa ggcgtcagac caaaagaaat caagcaacgt gtccaaaaaa ttgcagaacg
    14521 tttatccatc tctcatattt tggaaagcta tccttctgag atttccggtg gacaaaaaca
    14581 acgggtcgcc gccgcacgtg cattgatcac acaaccaacg attctattag gggatgagcc
    14641 tacaggtgcg ctggattcca aaagtgcccg ggatctttta gatacgatgg acgaattgaa
    14701 tacaaaagat catgtatcga ttcttcttgt gacgcatgat ccgttttctg caagttattg
    14761 ccaacgtatt ttattcatca aggatggcgg tatccatcaa gaagtgaaac gtggagatca
    14821 aagccgtgat tccttctatc gagaaatact atctattctt ggaaacttgg aacagtaggg
    14881 ggagattgac tatgctttgg aaattatcac ttactgggat caagagccgt ttaagagact
    14941 acttggtgtt attctcagga ttagttatgg catctgctat tttttacatg tttgaatcaa
    15001 tggcaagcaa cgaagctttt ttgaaaagta atacgatcgt tgcggctaca gttatcattt
    15061 tccgattcgg ttctgtttta ctagggatca tcacattcgt ttatatttta tatgcaaatt
    15121 cctttttgat gacgatgcgg caaaaagact atgcaatgtt tatgatgctg ggagcaaaag
    15181 gcagaaaaat cgcacagatg attttttctg aaacattctt agtagggatc gcagctactt
    15241 tagtgggttc agctgtcggt gtaggattga caagtatcgt ccaccgatta ttagtggatc
    15301 agttagatct caatatttcg cattttacac cttttagtat tcacgggttg ttgatcactg
    15361 ttttgttttt tgctgttttg tttttattag ctgctgttgt caatgctttt tcgattgtca
    15421 aaaaaccgat tttagcattg attcgtgcgg atcagacacc aacaagaatc aagcagcata
    15481 aattcttgtt tttccttgaa gtagtactgg gaattgcttt tctaggctgc ggttactata
    15541 tgatgaaaca cgtggggacc tatcaagtct ttggtatagg ggtagcctta gttacgatcg
    15601 tattaggaac ttatttcatt ttccattctg tgatcatttt ctttttatca ttactaaaga
    15661 aatcagaaca gatttcattg aaaaaattga acaatttcac attgtcacag ttaaactttc
    15721 gtattcgtga atatacacaa atgctctcaa tggtcgctat gctgtttgcc ttggcactgg
    15781 gcgctttgac ggtaggatta ggttttaaaa atgaaattgc agacttgaca gaaaacaatt
    15841 ctggatatga tctagtgatg aataatcctc gggcagaaga tcagcaaaaa atcaaagaac
    15901 tttcaccaac actttctgct tcttatactc aaaaagaaga tgcgggaacg atttactata
    15961 atgcaagtga atttgcggct caaccattga tcatgataaa acatgaagat ggaacaccgc
    16021 cgaagatcac gaaagaaaaa gttcctgcta aaaaaatgaa tgaactgact gtacaagagg
    16081 aacttcgaag ctatgaattg ccggaacagc aagaaaaaga aatcaaggta gtcagtcaat
    16141 cagaatttga tcaactgaac ttgccacaat ccactttaca gctagttcag gtaaaagact
    16201 tccaagcaaa cctgaaacca atcgaaacac ttgttgctca aaataaaatg aacaatccat
    16261 ctttgcaaaa agtagagtac agcaaccaga aatatgaaat gtatgaagtg tataatggtc
    16321 tattctctgg ttttgaattt atgggattct tcctaggttt agctttcttg accatgcttg
    16381 ctagctgttt gatgttcaag atcttgtcag gaagcaagag tgacattgct cgttatgaaa
    16441 tgcttgaaaa gataggaaca cgacgcgggt tgcttaaaca atcgatccgt agagaagtag
    16501 gcgtgttatt cttagcacca ggaatcttag gtgtagtcca tgtcttattc ggattacaaa
    16561 tgtttggtct attcatgcaa aatccataca aagatatttg ggttccgttt acgatattct
    16621 tcgtattgta tttcatctac tatgtactga cgacatggtt atatactgga atcgtattga
    16681 aaaagagatt gtgaaaaagc tgtcctgcat caagtaataa gaacagccaa gcaaaaatag
    16741 ctcctcatat ttttcgtttg gttgggctta ttaccgcaga tgcaagcttt tgaacaccgt
    16801 ttgttcggaa aaagaggttg tgacaatatt tttgtcacaa cctttttttc acggtctttt
    16861 attagaatat agtaaatttt ttctcgttac tcacggaata agctgttaag aaaacggttt
    16921 ccatgtatac tggaatcagt gcaaatgaaa gaaaagaggg tgcaaggaac atggaaatca
    16981 atgaacaaaa ttttggcgaa aactatcact tgattacgat cacgaatcaa aatgggacat
    17041 ctttgtctgt cagtgatctt ggggcacggg ttgttcattt tcaaacaata atcgataatg
    17101 aaaaacgaga gttgatctta ggttttgatg aagcaaaaga atatctagaa aaagaccctt
    17161 atatcggtgc ttcaatcgga cgtatggctg gacggattac gaatggcaca ttcaaattag
    17221 atgatgaaac gttcgaagta aaaacagacc cggacactgg tcattgttta catggtggtg
    17281 cacctagctt tgaagccaag cagtggcatt atacagtcat caatggtgaa aacgaagcat
    17341 cggtcatttt ctatcagact agcccagata tggaaaacgg atttcctgga actttagatg
    17401 tagaagtacg gtatacgctg acgaacgatg atacatggcg agtgacggtc caagggatca
    17461 gtgaccgcaa aacagtattc aatcctacaa atcatgtcta tttcaattta accggtgatc
    17521 cagcacagtc aatcgacgaa catatacttt ggttgaatag ccgattattt gctacgctca
    17581 ataaagatac aacgcctaca ggagaagtag tttcagtaag tggcacagca tttgacttcc
    17641 aacgtcctaa aaaattaggt gaggtctttg ccagtgattt tcgccaatca atgatagtgg
    17701 gcggcatcga tcatccattt ttgttgaaaa aacaatctgg tttactaaat gctgcggaat
    17761 tgacaagccc agatgaaaaa gtatcgatcc aagtaaaaac agatgcttca ggaatcgtgt
    17821 tttttacagc aaattttgga gaagatggac cggaaatgcg cgggaataaa ttagtaaatc
    17881 atggcggtat cacatttgag actcaagaac tgccaggagc ggagcgactt gaaagttttg
    17941 gagatgtcac tctggaacct aaccaagtac gcgagtttgt aacggaatac aaaataaata
    18001 taaagaaata gaataaatta aaaagggagt gtgacagtgt cacatcccct ttttaattta
    18061 ttctatttct ttaaaggcag atcaagccag aatgatttgt ctaaacttaa caaggttagt
    18121 acaggaacag ttgcagcttt aactgtacga gtagttgttt ctggtatctg tcttgccgta
    18181 aaattgttcc acaaatccca gattgaagcg acacggaaga tggaagaagc gttgttgacg
    18241 cgttttatcg ttgaaaattc taatcctgtg attggcacgc catctgttgt ccatttgatt
    18301 ggcacatcga atcgcttaga catattgagg atttcagcgt ttgcttcttc agttgttgct
    18361 gcttcttttt tcggttcatc atattttgtc aggtcgtacg cttcgatgat cgcgttcaat
    18421 ttttgtgaat attggcgatc tgtagcataa cgtccttcca aatatttggt tgcttctttg
    18481 tagtcttttg tttccgactt ccaagttggt ttgtagaaat caggattgcc agtcagtcca
    18541 tttttgatca agtcggcata gtcttccaaa ctttccttgt aagaaggata tttacggaat
    18601 tgtgcgcgga tttggtagct ttgtccggaa ctatcttgtt caagtgtatt gaaggtcacg
    18661 ctgcttcctt tatatgatcc tttgatacca aaaaggttat gatttggttc gcttgataat
    18721 aagctgtttc cagatccact ttcaaggata gcttgagcca tcatgactga tgcgtagatt
    18781 ccttcttttt gcccgatttc ctgtgcatca tctgcaatct ttttcaagaa ctgatcagtt
    18841 gtttggtttt gactgaaatg gaacgagcca tctaatccat ctaaaataga tgagccgcct
    18901 gtcagcgaac ctgaagcagc aggtaaacct gctaagttta ctcggacagc aaaatcaggc
    18961 gtgaaatctg aaatagaagt tactcgaact ttatctcctg gttgaggcgc atggataaat
    19021 tgtccgttcc caatatagat acctacatgg tgagtaacac cggaagaagg tccccagaag
    19081 acgaggtcgc caggttgggc ctgacttact ggaatctgcg tacctgcata ttgctgttcg
    19141 cctgtccagc caccgatata atgaccggtt gctttcatat atacgtaata agtcaaacca
    19201 gaacagtcaa aagtatttgg accttttgct ccccatacat atggttttcc aagatgcttg
    19261 aatgcttctc gaacgattgc ttgttggaca ttcgttgtat ttgtaggcaa tacagcatca
    19321 attggtgatt ttacaacaaa atcagaagaa gcagaagatg gattctctat accgctttga
    19381 tttgctgtat ttggacgaga tgaagcattg ttcgttccgc cattcgatga accattgttt
    19441 ggatggtttg gttctctctg agtatttcca ttgttattcg atccagatgg gtttgtttga
    19501 ttcgaagaat tcccgctgtt cgaattattg ttattattgt ttgaattatt tgaaccatta
    19561 ttattgttgt tattacccac atctggtttg tttggcgttt ccggatctgg tgtactaggg
    19621 acttcttcac taggatcagt tggtttctct gttgaagtat ccggtccagc tttaaattct
    19681 gcagtaatag ataaattgtt cgtacctact gtaaaggtct ttgcatcata atttacttct
    19741 ttggcaccat taacagcgac tgaagcaatt tgttcacctt cagctggcgt aatagtgtaa
    19801 gttaccttcg tttccgctaa taaaccttct accgttccac tttccgtaat atccaaaggt
    19861 tgctgttcag gggaatccgc tgtcgaagca gtcagttgga tcttaccaat caaagaagga
    19921 gcaattgtaa gggaatatgt ctcggctggc ttttcatttt ctgatggatc agccggatca
    19981 atttcagttg gctcggtcga tcctgttgtt gagttagttg ttccagttgg gtcttctgta
    20041 aaagaatccg ttgtttcact agtagctgag ctggatgtag tcgtatctgt tgtgactgtt
    20101 tgatcagcta tttgattatc tacggcaaat acggtattgg tcgacaaaac agacggagca
    20161 atcagccaag tagcagctag caaacgtatc gtttttttat ttttcataat aaactagtcc
    20221 ttttcctttc attgattttc tgtcttattc agcattcatt agacattttt tggaacagat
    20281 aatcgtgtcg aaaagctttt tcttttcata aatctgtctg tgtattctaa ataaaacaat
    20341 ttgtaaataa gcaacgagca ttcattcgtt tttctttgac gttcatttta ttgcatagac
    20401 cacctttgtt tggggataaa caagtgggga taaaaatggt tatgcatatg taaattatac
    20461 caaagtttgg aaagtatcac aaaaatttat aaaaataatg atcctaaaaa gaaaaagact
    20521 gaaatatgtg ataaacatct catacttcag tctttttcat taactattca taagacatta
    20581 ggtcttcatc aaacccgcta acgagagtag tgatcattgc tacgactaga ctaacagcca
    20641 gtcctgctaa gtaccagaaa aaagtaccgg aaatgaatgc aggcaatgtg gtcagactac
    20701 caaatacaaa agcgatcgct ttcacttggg caaatcccat tacagctcca ccagcagctg
    20761 caccaatgca agcagcaaca aatggttttt tcaatgggat cgcaattccg taaacagttg
    20821 gttcggtcac tcccgcaagt aaaatcgaag caaaagcaga accggagatc gaacgggttt
    20881 ttgaacgttt acttcgcaga aatacaccta aagctgcacc agccattcca aaattggctg
    20941 ctcctgtcaa cggaacgatt cggtcgaacc cataagcaga gatattactg atcatgacag
    21001 ggtttacagc ccattggata ccaaatatta ccaaaatatt ccaaccacca gcgatgattg
    21061 cgccactgat cggtccgtta atatccatca tccaagtaat tgcggcagct aaaccttcgc
    21121 caaggtaaac acccgcaggc ccgaatacca ttaacgtcaa aggaaccgtg ataactaatg
    21181 aaagtaaagg tacaaatact aattgcatat tttcgcgaat atattttttg aggaattttt
    21241 caacaaaaga ataaaagaaa atacctaaaa tcgccggcat gacagtagac gtatactgca
    21301 tcagtactac aggaatcccc agaaaatcag tagctgcttt gccactgtct gaaagtaaag
    21361 aggtcagatt cggttcaatc agtgctgcag caatgacagc cgcaatatat ccattcactt
    21421 taaacgtttt ggcagcagaa aaagcaagaa agattggcag aaaataaaag acggcattcg
    21481 acgcagcaga caaaatatga taagttccgc ttgtttcact aagccagcca atcgtagtcg
    21541 ctaaaacaac cagacctttt aatataccag aaccggcaaa cagcggaatg atcggcgtaa
    21601 agatttccga tatcaaacca aatagttttt cgatcggatt tagcttttct ttcggagctg
    21661 aatgatcgat ttgacgcgtc aattccttgt aagcaggagc caatccctga tccatgatta
    21721 tttctgcata tctatcgagt aaaatgaaat acttaatgga agggatttgc tgttttaatt
    21781 tttctgcatt ggctactggc tgttttttca atttgatacg aagccgagtc gcacatggaa
    21841 ggacagaatc aatattgtct tttcctccca actgttcaat cagcatatga atctgctcgg
    21901 acagtaccat ctgttcagga ttcattgtcg tttccattag aagaccactc cactcactaa
    21961 tataatattg gcgtaaggtg tcatttcgcc tgtacgaata aatgcatgac agttattcaa
    22021 ctcttttttc atttcttcat gtgggataaa gctaatcggt gtatctggta acaataattt
    22081 gattttttcc aatatttccg ggttcattgt ctttatttct tccgccaatt caatcgattg
    22141 tacctcaagt tcttctaaaa cgttttctaa aacagataaa aaacttggaa gaccgtttga
    22201 gacagccaaa tctatttttt cagtaccaaa ggggacaggc atgcctgcat ccccgatact
    22261 taatttatca aagtgcccca tttgtgcgat tactcgtgaa atatctgaat tgattacttt
    22321 tccttttttc atgtttgttt cttcctctca ttgttaattt tagactgctg attgttctaa
    22381 agcttcgcgg taagggatag aaggttgcgc accataagac tgcacagcaa cagaagaggc
    22441 tagatttcca taaaggatcg cttctttcaa gttactaagg tctggctgaa gtgtgctgct
    22501 taatgcgcca ataaatgtat cacctgcggc tgttgtgtca acggcatcca ctttaaaagc
    22561 agggacgata ccgtgttctt tttctgtatg atagaaagcg ccggcactgc ctagtgtaat
    22621 gattactgtt ccaataccta attcatggag tttttcggct gctgcgacca aactgttatg
    22681 atctgtcact ctgattcctg taatgatttc tgtttctgtt tcatttggta taatgatatc
    22741 tgtcttttca agtagttcag taggaatcgt ttcacgagct ggtgcaggat tcaggatcgt
    22801 tgtttttcct gcgtcttttg caatagaaaa agctaggatc gtcgcatcta agctactttc
    22861 aaactgagca ataataaagt cactgtttgt aatcagttgc ttgtttttta ggacttcttg
    22921 ttcatggaaa gcattatttg cccctgcatg gatcaagata ctattttctc ctgctgcgtc
    22981 taccataatc attgcttgtc cggttgtggc gccttggatt tcagctatcc cagatgtatc
    23041 gatgttttct tttgtcagta gatctaataa ctgttgtcct tcgctgtcag ccccaacgcc
    23101 tccaataaaa gatgtccgtg cgcctgaacg tttggctgcc actgcttggt tggcaccttt
    23161 acctccgcca gcgtgaaaaa tctcatgcgc atgcatcgtt tcgcctggct tgggcatgtt
    23221 ggttaaacga agggtagtgt ccatattaat acttccaatt actgtaaccg tgttcatttt
    23281 acaaacctcc aactatctca tttattttga tgggaaaatt attttttaaa acgttttagt
    23341 aaaacgtttt aatagttcga taaaaatata gcacaccttg aaagctattt caagggtgca
    23401 gttgaaaatc ttttttctaa tcgtacaggt aatctttttt cttcccattc tttttctggg
    23461 aattggatcc gatctaacaa taacttggca gatgtttgtc ccaattcgaa gattggttgt
    23521 gcgatcgttg tcagtttcgg cttgatatac tcgcacatat ctatattatc atagccaata
    23581 atgctgtagt cttcagggat gctttttcca gcttcctcta atccgcgata taatccaaaa
    23641 gctaattcat catttaaagc aaacacacca gttgcagcag aatcaagaag ttctgctgtg
    23701 atctggtagc caccttgttt tgagaactgt gtaggtaaga ggatcagttg gtcgtgaggg
    23761 atttggtaaa catcaagagc agatttaaac ccctcaatcc gagcgtggac attttccggt
    23821 ggattttcag gatagacaag ggcgatcgtt tgatgtccta gactaagcaa gtgcatgccc
    23881 gctaggtaac caccgcgaaa atcgtctgtt cgtacggcat cactaaatcc ttcagatttt
    23941 ttctgatcca aaacaatgaa gggccggcct tgttttttca aattttcgtt gattgcatct
    24001 gtagagacag cagaagtcgc gataatgaat ccatccacac ctcgacgtgt gagctcggct
    24061 agatattcga tttctttttg atgatcagaa tcagcattac ataatatcgt gacgaaattc
    24121 tgcttataca agatatcctc aattcctcgc atcagtgtac tgaagaatgg attcgtgata
    24181 tcaggaacga gtacaccaat tgttttcgtt tccttggtaa tcatttgtct agcaaaatag
    24241 tcaggctgat agcctaattc ttctttggca gctaaaactt tctgaacagt tttcggactg
    24301 aatttctccg tatttccatt taatatcaag gagacagttg tgattgaaac gccggaaagt
    24361 ttagcgactt ctttgattgt gatctttttt ttcaccatgt tcatactcct atctaaatcc
    24421 tgtggttatt ttcccatgaa atcaaaaaga agtaaatgaa aatcacgtgt gccagtactt
    24481 atttgctttg ttggatagct tcgattattt gtttcgttga gcgtactcgt gcagttttcg
    24541 gaaatatctt ttttatgaac aattgatggg attctttgtc acgatcactg caagcatctt
    24601 cagcaagcac gatatggtac ccgtgttgaa aggcatctaa tgcagtagca tatacaccgt
    24661 ttgttgtcgc tacaccgctt aaaatgatag tttcgattcc acgccggcga agttgaagat
    24721 caagagaagt accaaaaaaa gcacttggat tgtattttgt gatagtcaac atattatctg
    24781 tatctttcaa agaatccttc aaaagtaaat cagtgaattc tggaggtaca gaagcagtca
    24841 ttccacccat atcagtttgt tgactcaaat aagggaaagt ggagatgtct acgttcacta
    24901 aaactttcaa ggcagcggta cgtttcaatg ttcttactaa caaatcattg tttgtcagaa
    24961 tagatgctgc agtgtgtgga gagagtgtac cgttatttac gatctctttt tgcatgtcga
    25021 tcgcgacaaa agcagttttt gataaatcaa aaatcatatt ttcctttaaa tcattcatga
    25081 tgttccttct ttcaaataac aatgtttttt atgtaagtaa atcataaatc gtttatttgt
    25141 caactaaatt aaaggattcc tttctttagt ctagagaaat tcagtggtaa aataaataag
    25201 tgtgagaaaa cgcattcgat gcatagatat agcgagaaat atcgtgaaaa tggaggaatc
    25261 agagtgaagt atgtcgaagt aaaaaatgaa tacaaacgat acaaaatggg agaaacgacc
    25321 attacagcaa atgacgatat ttctttttca attgaaaaag gcgaattagt gattatactt
    25381 ggtccaagtg gagcaggaaa gtcaacgatt cttaatattt taggtgggat ggatacacct
    25441 gatgaaggtc agatcattat tgatgacaca gatattgcgc aattttcaga taaacagctt
    25501 actgcttacc gacgaacaga tgtagggttc gttttccaat tctacaatct cgttcctaat
    25561 ctaacagcaa aagaaaatgt ggaattggca acggaagttt ctcctgatgc gcttgatcct
    25621 gtagaggtat tgaggcaagt gggcctagct catcgattga ataactttcc gtcccaatta
    25681 tcaggaggcg aacagcagag ggtttcaatt gctcgtgcac tagctaaaaa tcccaaactt
    25741 cttttatgtg atgaaccgac aggcgcgctg gattttgaaa cgggcaagca agtattgaaa
    25801 ttattgcaaa acgctagcag acaacacggc aataccgttt tgatcattac acataattct
    25861 gctttagcgc ccatcgctga tagagtcatt cacattaatg atgctaaagt aaggtctgtt
    25921 gaattaaatg atcatccttc atccattgat gagattgtat ggtaggggga ttttatgaaa
    25981 acaagaacat atcttaaagc aggttttcgc gaaattcggc aatctaaagg acgattcatt
    26041 gcaatcatcc ttattgtcat gctaggaaca cttttatttg taggtgtcaa aactgctgga
    26101 cctgtcatgc aaaaaacgat ggatcactat gttggtacag caggactttc tgatttgcaa
    26161 atcgtttcta ctggtggctt gacacaaaaa gatattgctc aagcagaaaa gatcccagat
    26221 gcaaaagtag aaacaggaaa acagatctat tattccaata ctggcaaaaa cgaagtgatt
    26281 caaatatttt cttataacaa aaataacaag cagaacaagc tacaattaac tgatggaaaa
    26341 ctcccggaaa aaccaaacca gcttgtattg gatgaaaaag cggaagatga agggtataaa
    26401 atcggtgata tttatcagat tgattcagat gaattgaaag aaaaagagta taccatcacg
    26461 ggctttgtcc gttctccatt gttcatcaac aatttggaga gaggatacgc gaacgtcgga
    26521 aatggtagcg tcgattattt tgtctattta cctgaatccg tttttaaaac agatatttat
    26581 tctgtcttat atcttgattt tacaaacgta aagaatttga atacgtacag caaagcttac
    26641 aaagataaaa tggagcaaaa tcaagaaaag gcagaaaagt atttacaaga tcgacccgaa
    26701 gaacgcttag aagagctgaa aaaatcggcc aacgaagaat tagaaccggc acagcaaaaa
    26761 atagcggatg gcaaagcaca aacaacgcaa gcaagaacac ggttagaaga tgctaaaaaa
    26821 cagttagcgc aacagtcagc aactatccag cagcttcctg aagcgcaacg aacaatcgct
    26881 gttcaaacat tatctcaaca agaagctcaa cttgctgaac aagaaaggca attagttgag
    26941 aaagaacgtg aactggcctc tgcagaggaa gaattgaatc gaaatgcaga aaaaattgaa
    27001 aatttaacta aaccatcata cctatacaat gaacgttcag aaaatgtcgg ttttcaagaa
    27061 tttggtgatc tgtctgatcg tatcgctgcg attgccaatg tttttcctgt tttctttttc
    27121 tttattgccg cattgatcac ctttacgaca atgacacgta tggtagaaga aaaccgcagg
    27181 gaaatcggga cattaaaggc gttaggttat acgaaagttg aaatcgcaaa aaaatatgca
    27241 atctatgcaa gtctagcctc agggataggt attgttttag gaacgatatt aggaacgaat
    27301 cttttgccaa ggatcatttt tgagctgtct aacgagcggt atgatattgg gagtgcgctt
    27361 attttttatg actggccgcc gattttacaa gcagctgccg cattcttcat tgccgcattt
    27421 ggcgctgcaa tgatcgtttt attcaaagat ttaagagaac ggcctgctgc attgcttcaa
    27481 ccgaaagcac cgaagccagg gaaacgcatt tttcttgaat acatcactcc gctgtggtca
    27541 cgccttagct ttaatcaaaa aatcagttac cgaaatctct tccgatataa gtcaagaatg
    27601 cttatggcga ttatcggaat tgctggatgt gccgggctga tggttgcagg cgtgggattg
    27661 aaagattctc taagttctgt ttcagccaaa caatttggac cgatcattga ctatcaagca
    27721 atcgttacat tggattcatc cacggaagat agtcgtcaaa aagcagcgga tatgctgcag
    27781 aagcaagaaa aagtaaatga gatcctagct attcatactc aaaccgtaga attgagaaaa
    27841 aaaggacatg cttcgcaaag tctgacagtg atcgtaccaa gtaaaacagc tgatttggat
    27901 cgttttatcc atttaactga tcttgaagag aacccacgct ccttgcctaa aacaggaatc
    27961 gctattacgc aaaaagtagc agaactattt gacttatcta caggagatca tatttcgctt
    28021 tatgatgaag atcaaaaaga atggaaagtc gaagtaaaag aaatcgtgaa aaactatcta
    28081 ggtagcttta tttatatgtc ccctgaatat tatgcttcag ttactgatca atcgatgatg
    28141 gataatgcat ttttgctgcg tacagataag atgacaacaa aagaagaaga aatgctttcc
    28201 gagaaactgc ttgccacaga cgctgtgaca aatacgtctt tcatgtccaa acaaatcgaa
    28261 acgcaagaag aatcaatggc gaacttagac tcggttgtct taatctttgt cgtattatca
    28321 ggactgctgg cttttatcgt cttgtataat ctaactaata tcaatatttc cgagcgtgta
    28381 cgcgaattat ctacaataaa agtccttgga ttttttgata aagaagtcac gatgtacatt
    28441 gttcgagaaa atatcatctt tactctttta ggaatcatcg gaggatttgg tgtaggatat
    28501 atattgactg acttcatctt gcaacaagcg tctatggaga atgtcatctt tccattagtg
    28561 atcacatggt gggcttatgt cttatcagca ggattaacta tcgtatttac ggttattgtg
    28621 atgattgtta cccatttcaa attaaagcat atcgatatga ttgatgcgtt gaaatcgaat
    28681 gaataaaaac agaggttgtg aaatcagcgg tttgacctga gtattagaga aacaaacggc
    28741 aaaatagttt ttcatatttt gccgtttttt gagctaatac cgaaggacag ctgattgaac
    28801 accatgaatc agaggttgta aaaaagccgt cctgcatcga gcaaaaagaa tactcaaata
    28861 aaattagctc ctcatgtttt acatttgaga agactgattg ccgtagataa aagcttttga
    28921 acaccattta tagaagaaac aagaatattt tgctatttta aacaagagga tagacaaata
    28981 catgtctgat ctcttgtttt ttattgggca acagacctct tagaatgtat aaagataaat
    29041 tttacaaacg ctttcggttc agttataata ataaatgata acttgtatat ataagttgtg
    29101 gtaggtaata aatcagttga atggaggtaa atatccatga catttaaaga caaagtcatt
    29161 taccaaatct atcccaaatc ttacaaagac agtaatggtg atggaattgg agatttaaaa
    29221 ggaatcaaag aaaaactccc gtatctgaat gatctaggca tcgacatgat ttggctaaac
    29281 cctatttatc caagccctca aaaagacaac ggttatgata tctctgatta cacagccatc
    29341 gatcctatct ttggtacgat ggaggaattc gaacagctaa tagccgaagc aaagaaatac
    29401 cggatagaaa tcatgctcga tatggtgctg aatcacgttt cgacagaaca tgaatggttc
    29461 aaaaaagcac tagcagggga ccagcattat caggacttct tcattttaag agaagagcca
    29521 acggattggg tatcaaaatt tggtggaagt gcttggtcgc catttggaga tacggggaaa
    29581 tattatttgc acttatatga tcggacacaa gctgacttga actggcggaa ccaagcagta
    29641 agagaggaac tattcaaagt tgttcgtttt tggatggaaa aaggggtaaa aggattccgt
    29701 ttcgatgtta tcaatgttat cggtaaagac gaacaattga aaaataataa agaaaatgat
    29761 ggcaaacctg aatatacgga caaaccaatc acccatacgt atctaaaaga attgaaccaa
    29821 gcaacgtttg ggacggattc cactatcatc acagtcggtg aaatgagctc tacgacgatc
    29881 gaaaattgta tcttgtatac agaacctagt agagaagaat tatcaatggt ctttaatttc
    29941 catcatttga aagtcgatta tgaaaatggg aataaatggt ctactcctcc atttgatttt
    30001 gcagaattaa agcgactatt tcatacatgg ggagaaaaga tgagtgaggg taacggctgg
    30061 aatgctctat tttttaataa tcatgatcag ccacgtgcgc tcaatcgatt tgtagctatc
    30121 gatcagtatc gaaaacaagg agcagagatg ctagcaacca tgatccattt gaaccgtggg
    30181 acgccctata tttatatggg agaagaaatc ggcatgatcg acccagacta ttcatcgatt
    30241 tccgactata ttgacgtaga aagtttgaat gcttataagt tactccttga acaaggattc
    30301 acagaaaaag aagcgtttcg tcggatcaaa gcaaaatctc gcgataactc tcgaacaccc
    30361 atgcaatggt cagacacaga acaagcagga tttactacag ggaaaccttg gttaaaagtt
    30421 ggaggcaagc tagaagagat taatgtagaa aaagaactat cttctgaaga ttctattttg
    30481 tcctattaca agaaattgat tcggctgaga aaaacatatc caatcgttgc acaaggagat
    30541 tatcatgctt atgaagcaaa tcatccggaa gtttatggct ttcttcgcca atttgaagga
    30601 cagcagctgc ttgtattaac aaatttctat gataaagata caaggatcgc catcccagaa
    30661 ggattcacag aagccaatat tcttatcgat aattattccg atactgttct tcatcaaagt
    30721 ctggagctaa aaccatacca ggcaattgct ttgctgaaaa attaaaacaa aaacacaaaa
    30781 cgtcgttcct acgagctatt ctcgtaagat cgacgttttg tgttttattt aaaataatct
    30841 tcttcagaaa atgaagagtt ttcatactct cctttatcgc cataaaattt attgtagcgt
    30901 tgtttgaatt tacggaaaat aaaagtaacg agtaatttga ctaacgtata gataggaata
    30961 ccaaaaataa cgcccacaat tcccattaga tcacccatga caagtaaaac gattaagatc
    31021 gtaatcggat ggatctgtaa acgatcaccc attactcgag gaatcaccaa gtcaccgtgg
    31081 agaagttgta cggcaaacca gacaatgaca aatttgacag ccataaaagt agagtcttgg
    31141 aatgcaatga tcaaacctgg aaagaaggcg ataaacggcc caatatatgg aatgatacat
    31201 aacaacccag cagaaatagc taaaacacta gcaaattcta atccgatcag taagaaacaa
    31261 acccagtaga ctgctccaag gataaatgaa gcaatgattt gcccctttag aaatgaaccc
    31321 aattgttgat tagcaatctt tgtcaaattt ttaaaatctg tacgaaatgc tggtgggaca
    31381 atcgctaaaa ctgactgata aaatttttga ggatctctca aaaggaaaaa agcgacaatt
    31441 ggaccagtaa ataaagtaat aaaaatagct gtaacagttg aaaaaatgtt gctgatccgt
    31501 tgtgcgccac tttcccaata atttccaata aatccagcta tatcagacgt cacgtcgtca
    31561 atagaagcga aaaattgatt aaatgaatcg gcaaaaggtg ttttctccaa aaattgtcgg
    31621 agactctctt gaaaatcttg aaacaaatct ggcaaactac tgatcaatgt ctttgcctgt
    31681 tcttcaagaa aaggaataag ccatatgctt ccaagcccca ataaaaataa aataatcaca
    31741 taaacgataa tgactgacaa gttcctcgaa aggtgcttgc tcaaacgttt gatgactggt
    31801 tccaaaagat aatacaagat cagtgcaaac aataatgggg gcaagatagt agcgataatg
    31861 attccgacag gcttgaatat aaacgagatc tgatggaaaa aataaatgat cagcgcaagt
    31921 aagataacta ttcctaaaac atagtaagag gtttttcctc ctaaaaagcg gatcaaacga
    31981 ttcgttgtat ctgtttcttt tttcactctt tttcctcctt tatctataaa ttcattatag
    32041 acaaaagcgg gtataaaagg aaaatgatag aggttgattg caaaaatatc gtcaatcatt
    32101 catataaact ttctcatcaa ttaaaataag aaaacgcttg acagttcttt taaccatatg
    32161 ataaattaaa tcctgttgct aaaacgtaac tattacggtt tgcgaaaagc gaatcggaac
    32221 attcaaatga aagaagatga gaagaggagt gtcataaatg aaacgattga taggtatatt
    32281 ggccatgctg gttttagcag gtatgttaac tgcttgcggg gcaagtggga aagccgagga
    32341 cagtaaagaa aaattgagcg tgatgacgac cttttatccc atgtatgatt ttacaaaagc
    32401 aatcgtcggg gatgaaggag aagtagagtt attgattcca gcagggactg attcccacga
    32461 ttatgaacct tctgctaaag acatggcaaa gatccaagat acagatatct ttgtttataa
    32521 tgatgaaaac atggaaacat gggtccccgc aatccaaaaa acattacaag aaggcaatgt
    32581 ccatacgata aaagcgacag aaggtatgct tttacttccg ggaagtgaag aaggacatga
    32641 tcatgaccat gaacacgggg aagaaggcca tacacatgag ctagacccac atgtatggct
    32701 agctccaagt ttagcaatca agcaggtagc aaacatacgt gatcagctga ttgaagctta
    32761 cccagaaaaa caggaagtat ggacaaaaaa tgcagcagcc tataccgaga aacttcaagc
    32821 actccatcag ctttatcaag aaacctttaa gcaagcaaaa caaagatctt ttgtcacgca
    32881 acatgctgca ttcaactatc tagcgttaga atatggattg aatcaagtat cgattgctgg
    32941 tctttcttct agtgaagaac cgtctgcggc gagaattgct gaactgaaac actttgttaa
    33001 agaacatgga atcaactata tttattttga agaaaacgcc aaagatagta ttgccagaac
    33061 gttagcaaac gaagcaggtg tatctttaga agtattgaat cctttggaag gattgacgaa
    33121 tgaacaaata gaaaatggag aaaactatct ttctatcatg gaagcaaacc tcgaagcgtt
    33181 gaaaaaaacg acagagacag aaaacccgct agaagatact ttgaccccca aaaaagaaaa
    33241 gagcgtctac aacggttatt ttgaagacga ggatgtacaa gatcgctctc tttcagattg
    33301 ggcaggaact tggcaaacag tagatacgta tgtagaagat gggacttttg atcctgtttt
    33361 tgaatataaa gaaaaattga atcaggataa gacagcacaa gaatataaag aatattatac
    33421 gaagggctat caaacagata ttgcttcaat caagatcagt gagaaagaga tggcatttac
    33481 gtttaagaat ggaacaacga aacgttcaaa ttatcgttat gtagggaaag aaatcttgac
    33541 ctacgaggca ggaaaccgtg gcgtaaggta tctattcgaa gcagaagata aggaaagtgg
    33601 cgcttttaga tatattcaat tcagtgatca cacgattgca ccggctgact ccgaacattt
    33661 tcatttatat gcaggaaatg aaagtcaaga agtattgctg aaagaaatgg agaattggcc
    33721 aacattttat ccagatacaa tggatggacg tgaaattgca caggaaatgc ttttgcactg
    33781 ataggcaaaa aagaaccgaa gggtttggat cggctggtca tttagtcggt ccaaactatt
    33841 cggttctttt tatgaaatca atttctttaa tccacggata gaggctggga tggtctcgcc
    33901 gtttgccaac gtgacttctc tggttttttt attaatcgac cgcacatttt gtgtattgat
    33961 aacataggat ttatgacaac ggataaaatt ccgatgcatt ttctcgattt ctttgatctt
    34021 ctcatagaat tgtaattggg catgagtcaa atggacttcc agtttatgtg gcgctcctag
    34081 cgaagtagaa aaatattgga tgtctttgat cggtaaaaat ttgatctcat cattaaactc
    34141 tattttgaag atatcttttt ggatttggga catttgaaaa tcttcgaaaa ttttgctcag
    34201 gatatgctcg acattttctt ttaaatcata aagatcttct ttgacgatat aatcagtcgg
    34261 ttcgatgttg ctttttaaga ttttcatcga catttccgta tgggaagtca catagattat
    34321 ttttcctaaa ggatctaatt cacggatcaa tttaccgatc gctacaccat ccatttcact
    34381 gtcttctagt tcgatatcta aaaagtaaat accaaaagaa gaagttctga tcttggctgc
    34441 attgagaagt tcttccggat cagaggtaga aatatggaca taactgtcta agttttcaat
    34501 catgattcta tttttgatca ccgttgataa aacttctaat tgttttggat cgtcttcaca
    34561 aatataaatg ggaatcatat cattactcct ttattttatt tgtatataaa tcgtttgaga
    34621 aaaacgctgg ttttcaattg atgtttcaag gaaaagataa ggattggtct ttgtcaattc
    34681 atccaagata aacaagccaa ggccttcgtt ttttggattt gtttttgttg agaacccttt
    34741 ttgtttcaac tggtttaacg gtaatgtatt tgttgttgta ttgatcacac gaatcatcac
    34801 ggaatgattc ttcttgagta tggtcaattc gatttttttc ttctttgcag cactagctcc
    34861 ttcaatcgca ttgtccaaga taataccaag catacgtaca aggtttaccg tatgtttcgt
    34921 atccagttcg attggctcag gaaaatgaag ctggacgatc aacccttttt cctgtgccat
    34981 catgagtttt aatgacagta aactacggat ctcaggaact ttcatattat ccaacatact
    35041 tagattggcc ggaaaaaggt tcatcatctg gcgtgtaggt aatatcgttt gatcataata
    35101 agaccgtaat ccttctaagt cgccttctga aatatagctg tgaagagaaa aaagcagatt
    35161 ttggtaatca tgtttgaatt ctctcaagtc ttgatagttt ttttctagat ctttgatata
    35221 atcctgttgc atcctgactt gttgctctaa taactgatca gctaatttct tattagagat
    35281 tttccaataa taaaccaaga tgatcaggcc aacacacaga tatagaatga aaaaactcgt
    35341 attcaaagct aagacatcag gtgtttcacc agaaaaacgg gtataaaaaa taatgatata
    35401 gtatgtaaat aatatcaatg tcccgatcgt accgatcata cggcggtcca cagtgtgaaa
    35461 ccaagtctct aacgattttt ttaaacaaaa agctaataaa gcagataaaa aagcggataa
    35521 gacaatgtga aaatactgca atgaatctcc tggttcaata accgaagatt tcaaaacaaa
    35581 aaaatcgccg atgcttgtta agtgatctcc taaaagcgac gtcaataacc caaaaggaag
    35641 cgcgtaaacc aattgttgac gcgaagtcga aaagaagaaa aaagtaagca tcagcattcc
    35701 tatagcaatt agagcagtaa aaatccctaa aatcgaatag acccatccaa tcaaaataca
    35761 agaaatcaga atgactcccg caaacagata tttgtatcgg atgtgatggt caatcaataa
    35821 gatacaaatt gtgtaaatca aatattgcaa cgctatggac cataaaaaca taaagtacag
    35881 ctcccattga tttttaccaa attatagcac aattggcgta ttattttttt aatttttcag
    35941 ggattttgaa ttcagaactc ataccaaagc agcagccaga tacaaaaaag tcagataagt
    36001 tagttaattg tttaatgatt gtttttttca tgaaagattc ctccaaaatt catcaagtag
    36061 tttgtaatag gaagcaacgt agctatttct aaaacaatcc caagtagcaa ataggtagaa
    36121 aagtaagaag ctgagaaaaa taacgtaatg agagaaaaaa ttagaaacat gctgatcgcc
    36181 ttctttaata atgggggacg tttctcgggt tcaatgggat tgattgctgt acctcttgat
    36241 ccatagcgaa gcagtaacaa aaagcagctg attaacaaaa agaccatcat tgggaaggag
    36301 atagtcaatt gcagatgttt catcaccaag ggaacgcccc ataaataaag tagtgtccac
    36361 agcagacaaa cgaattcaga gtcggaatgc aatccataag catatctgcg gatgcagata
    36421 taagaaagaa aggtgattcc tgtttccgcc catgagcctg tcaccagaga aaagactaga
    36481 acgaccgcta gttttccaag attattcaat aaaacttcta agacaaattt tacttggaca
    36541 tacaccatat cttcttccga tttgtctccg ctgaataagg cttctaataa tttgttcaca
    36601 actttatctt tcatctcata cgtacctcca ccacaaattt tatgctaaaa aaaataaaaa
    36661 acgtcttttg tttcataaaa agtggttttg acatcaaaac cgtcatgatc tcctaaaaaa
    36721 atcttcgttt tccaagaacc ttaccaaaaa aagtgacagt tttgatacga aaagtacttt
    36781 ttatgacaag tttgtgcttt ttcgagtgat ttatgttaaa aaattactga ccttataaaa
    36841 ggtgatggag ggattcttat ggaaaatgaa caattgtttg aagtcggcga aacatatgca
    36901 tttaaatctg atttatttga atcaccaata aaaggaaaaa tagaaaaaat ctatgaaaat
    36961 agtgtgatga tacgtgtcat gaaatgcgca gtagaagaca atgataccgc tcataatctg
    37021 cactggcagt tagttgtacg caaatcaagt acaatagaag aataaaaaca gataaaatgt
    37081 tgagagcata aagaggttgt gagtctcccg tttagctctg agttacaagg aataatgtta
    37141 aaaaacagct tttcatgttt tatgaatatt gccttgtgac cgaggtgttg gcaggagaat
    37201 actgtttatt tgaaaaaagg gctgcgacaa gacttttgtc acagccttta ttttcatgaa
    37261 taggagttac ttttggctac ttttagagaa gtagctttta aggagcagat aaagtcaagg
    37321 cttttatttc tataaatgaa gtagtaaaat gaagaacaga ttttattaca ggaaggatgc
    37381 cgataattgt gatcattgtt gcaggaacga tcggtgcagg gaaaagcaca ctaactgaaa
    37441 tgcttgccca agatttagaa acaaaacctt tttatgagaa cgtggaagat aatgaagtat
    37501 tgccgctatt ttattctaat ccagaaaagt atactttttt attacagatt ttctttttga
    37561 acaaacgctt tttggcaatc aaagatgctt tcagccatga tgacaacgta ctagaccgct
    37621 ccatctacga agatagtatg ctgtttcatt tgaatgcaga tttagggcgt gtttctgaag
    37681 tagaagtaaa gcagtatgaa ggtcttttag aaacgatgct gaaggagtta gaagaaattt
    37741 cgccacaaaa gaaaccagat ctactggttt atattcgtgt atcctttgaa actatgttag
    37801 cgagaatcaa aaaaagaggc cgggaatatg aacagctaga acaagatcct gaactctatt
    37861 cctactataa agaactcaat cgacgatacg aagagtggta tgaacagttt gatatatgtc
    37921 ccaaaatagt gatcgatggg gacaaatatg atttcgtagc cgatccggca tgtggagaac
    37981 aaatcgtaca agaaatcaag atgcgggcaa agaaaatgac agaagaggca gatgcccagt
    38041 cgatccgcta cactacaaat aacccgaaag caaagggaat ctttacaact aaataatata
    38101 aaaactaagc aaattgctca ctcgcagata tttctctgtg agcagcatct tgcttagttt
    38161 ttttgattca ttgagatcaa atagtgaacc gattcgtatg ataaaattgc cataaatgaa
    38221 atgtctgagt gagggccatg atcaaaaata aaatggaaac gatcaaaatc gaatgggcgc
    38281 caaacagatg atgacagaaa acaaggacaa tagctcctgc gataaaggca agaacgacac
    38341 ttagatagtg gaaaaatcga tcaaaatctg ctttttcttt agaaatacaa taatcatata
    38401 agttcgttgc tacccctttt aaatttccag ttgtaaataa attagtgtag gcacgcccgt
    38461 tgattttatc aaaagctacc cactggatcg atgcagtaaa agcaatcaaa atcgttacta
    38521 atgcgggatg ctcatggacg ggtgtcaaac taacaaagaa aaaaatcact gcttcataat
    38581 atagaacaaa caagcgccag aaatgttttt ctttttttct aaaataatca attagatact
    38641 ttgttagaag aatccctaag aaaaagaaaa gggtagacaa tactttttta ccaacactta
    38701 gccattctcc gtcaaatgct tggatgatcg atagaatgat attgccagtt tgagcagagg
    38761 caaaagcacc atattggata taagtataac tgtccatggc tcctccgata aatgtcagca
    38821 aaagtcctat tgtacgcccc tcgtgaaagg aagtctgcat ttttttcatc accttttatg
    38881 aatatttttg tcttccttat cttagcataa acgattgaaa aaaaggaaaa attttcagaa
    38941 aactaaaatc atatcgcttc ccattttcat ttttagaaag gtctgttagt ataaagaaaa
    39001 aatagacaac gaaactttaa ataaccaact tgaaataaac aaattcaaag aagaaagatg
    39061 ggaaggaaat caatcagttg aatgaaaaag aaattctccg attacaaaaa gaattgaaac
    39121 gctgggagaa caagtataaa aagcttcaac aagtctttcg gctacaagaa gaaatcattg
    39181 ctacctatga agcggcttat ggcgaagcac ccggacttat tgaggaagaa gtacaagtaa
    39241 aagaaagtga aggaaacagc tctttcttta aaatgatgga gaaacgttcc tataatcgct
    39301 tcaatcaaga acaaaaagca gctatcacat atgatatgaa gaaacatctt cgaatcatag
    39361 caggagctgg gagcggcaaa acgcaaacaa tctgtgcaaa agctgtttat ctgatgacgc
    39421 aaaaacaagt agacgaagaa cgcatactaa tgatcacttt tacaaggaac gctgcaaatg
    39481 aactaaaaaa acgagtagat aatttcagcc agagaaaaac cgctgtccat atcggaacat
    39541 tccatagtat cttttttagg atatacaatg aaatttgtcg aaaatttcct gaagtagcaa
    39601 tgcaaggaat acaaggagac ttttcaaagg atactgcaca aaaagtgaat gctgttttgc
    39661 aacagctgat acgtaaatat aatctttata tatttgatca atatggagaa aaaacgattg
    39721 cttctcggtt agactattgg cagaatatga acctttcagt agaagaaatg gttcagttag
    39781 ttaaagaaaa atatgattcc attgataaga atgcacgaca gccgatatca gaacgactgt
    39841 atggattgct cacggaactc caagaacaaa aaagaagcca gcaattgcta gagttcaatg
    39901 atgtactcca aaatctgaaa agcgctttgg agaatgagga gatccagcat tatatcggcc
    39961 aaaaatatga ttatctattc attgatgaat ttcaagatac taatccactt caatgggaaa
    40021 tcgtgaaacg gatgacaaag gaaaacagga tcaagttgat tgttgttgga gatgatgatc
    40081 agagtatcta tggattcaga ggatcagagc cagcatacat caagaacttt gaaaaagaat
    40141 atcctaccaa aactcttttc ttattaacta attatcgttc tcgggcagct attgtccaag
    40201 gagcaaatag attgatcagc tataataaaa atgatcggat accaaaggcg atgattccag
    40261 cacaaaaaga agcaggtgtt atggaagcct atttgttttc tgatactaaa gcagaaagtc
    40321 agtggctaat caaacaaatt cagacattta ttcaaaaaaa tgggaaatat aaggaatcaa
    40381 tcatattata ccgatcagct tcacaaacgc agcagcttgt ccaatctctg ctgaaaacga
    40441 atattccttt tgtactggaa gcagatagtc catatgaagg tatctttggt atcaagtctt
    40501 tccaacattt ctatcaaaaa attgttcatt ggcaaacagc actgaatctg caaaagaaaa
    40561 atcaggccta ccagcaattg ctccggcagc tgatggcaga ctgctacctg aaaaaaagtg
    40621 aatgcgatca atacttttct cctaaatatc aaaatatagc cgttgcagat tatattttgt
    40681 cgataagccc caatttaaaa tcgaaatctg cagaaatccg agagtttgag gaagcgttgc
    40741 gtaaaacaga ccagcatcaa gaaatctatt cgcttgttca aagctatctt catctgccac
    40801 gtatagctaa ggaaatcgac cagagtgatc aagaatggat acgagaagaa gtaaagcagc
    40861 atgctacttt cagaagactc caagccttat cgcaagaaac ccaagcgaca gctaaagaat
    40921 taaaaacccg cttgattgct tacagacaag gaaaacttga tgcactatgt atccagagca
    40981 ttcacaaaag taaaggtctt agttaccgaa atgtcttttt gattggttgt aacgaaggaa
    41041 gtttgcctta taacggagcg acagaaaaga aagtaatcga ctttaatgaa ataaaagcag
    41101 aaccagtaac gacgatagaa gaagaacgaa gactatttta tgttgcgatg actagagcaa
    41161 gaaatcgtct gtatctatgt gtacctcaaa tgaaaaattc aagaaaattg aaagtatcga
    41221 gatttatcaa agaaaccggg agtcgtgtga aaaaaggaaa gtgatagaag agagggtgtg
    41281 acagaagtgg tcagtttcaa gaaagaagaa ggaatgtacg aaaattgctt ctcaaatttt
    41341 tgtgaaattt cagcttattt tcgaagaagc tatttctgga acacagttta ttcggaaaag
    41401 ggaggtgtga caagaatttt gtcacacctc ccttatttat tttgaaaatg agaatactat
    41461 tatcaaaata gagaatatat agaaaaattc tcattattca aacgtaaata atcagttatt
    41521 ttgatttttt ttatatttta ttagctaata attggtattt taaaagaaat tactgtagaa
    41581 ttggattata attaaaaagg agtgagaaaa tgataaaagt atatacttcg cctagctgta
    41641 cttcctgtag aaaagcaaga gcatggttgc aagcgaatca acttgaattt gaagagaaga
    41701 atatttttgc cgaaccgtta acagaaagtg agatcaaaaa gatcttggca gcgactgaag
    41761 gaggagttca agatattatt tccactcggt cgaaaattta cgaaaagctg ggcatagatt
    41821 ttaacgaatt aactttaaag caaatgatcg tcttattcaa agaatatcct tcattattga
    41881 gacgacctat tttgattgat gaaaaaagaa tactaatcgg atttaatgaa gatgagatcc
    41941 ggtcatttgt ccctcgtgaa attcgatgtg ttgccagaaa tctgatctat ttacaaaaca
    42001 ataacatctt aaattcatag ggagggagcc ttcgtggaag tgaaagtcta tggatcagcg
    42061 cactgtgtaa caagtctgaa ggcgaaacaa tggttagaac accatcaatt gccttatcaa
    42121 tttgttgact tagaagaacg cgatctaagt acagaagaag cagaagaatt atgcagtttt
    42181 gaagaagtgc aagcagaaca gctatttgcg acttggtctg aggcttttca caacattgcg
    42241 attgaccttt cctgtgtagg aaaaaaacaa ctgatttttt tatgcacaca acataccaat
    42301 ctcctcagac gtcctttgat tattatcgat gaagttctat ttattggata caatgagcag
    42361 ttgatggaac agcttatctt aaataaatag actttaactg ttttaatcaa gtcactgagc
    42421 atttcataca gttgtaacag tcgaacactt agcatcaagc gtgttatgtg ttcttttttt
    42481 gactttattt ttctactata gagacatgct caatttatgt ggtcaaaatt caaatctagg
    42541 tggggatata gagttaaaaa tatattgaca tgtgtaaatt tacatgatat attcatttct
    42601 gttgttttaa gtagcaaagc aataaaatag tattttaaaa aacatataaa atagttgttg
    42661 acatgttaga aatgacatga taagataact gagttgttaa aaaagaacca cttgtgaagc
    42721 ttgattctat cgaaaaaaac ttcaaaaaag ttcttgacaa ataacaaatg ataagttatg
    42781 ataaatgagt tgctgtaaga tttcgaaaaa cgaaatcaaa aaaactttta aaaaagagtt
    42841 gacaattact tgtctctctg atatgatata aaagttgcta cggcagccaa aaacg
//
LOCUS       DACGLE010000016        42763 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0534
            SAMEA3304449-rid9560453.denovo.017, whole genome shotgun sequence.
ACCESSION   DACGLE010000016 DACGLE010000000
VERSION     DACGLE010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304449
            Sequence Read Archive: ERR985064
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 42763)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42763)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702225.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702225.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 20:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,045
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 27 of 67
            Pseudo Genes (incomplete)         :: 25 of 67
            Pseudo Genes (internal stop)      :: 19 of 67
            Pseudo Genes (multiple problems)  :: 4 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42763
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304449-rid9560453.denovo.017"
                     /strain="VREN0534"
                     /db_xref="taxon:1352"
     gene            complement(5..628)
                     /locus_tag="IXT20_000995"
     CDS             complement(5..628)
                     /locus_tag="IXT20_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4859462.1"
                     /translation="MRKKRPFLLFWLFGFFLLLILSAAFSGKMTADLAQKETEKIVAS
                     KESVDNFKKEFSTYWWAEYKITANYEKGELDIYLPKGKEENQFINIDDVLSAISLRQY
                     QFKDKHLTVLLLTHDGDKVARLKNGKVITAYSEKVDKKVVADRLAEWKKTNEQVKKTT
                     VSSQKNESTGTSPSDSGSGQTITGSEENQASIITNESSQGQYQSYGQ"
     gene            complement(787..2184)
                     /locus_tag="IXT20_000996"
     CDS             complement(787..2184)
                     /locus_tag="IXT20_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAQ4859463.1"
                     /translation="MKKYQFPANFWWGSAASGPQTEGRISGDGKGENIFDYWYKKEPQ
                     KFFDQVGPEKTSQVYTKYQEDVQLMKQTGHNSFRTSIQWSRLIPDESGTINEEAVRFY
                     HRYFDELIENGIEPFVNLYHFDMPMYLQEKGGWLNRETVEAYEKYAKSCFELFGSKVK
                     KWFTHNEPIVPVEAGYLYRWHYPEESDMKKAIQVGYHEALASALAIKSYHEMEQGGVI
                     GIILNLTPSYPRDESNPEDVKAAQIADAFFNRSFLDPAVKGEFPSELIEIVKELDLMP
                     VIHPEDLTVIKENTVDLLGINYYQPRRVKAKETPIDTKNGPMPEDYFDNYEMPGRKMN
                     PYRGWEIYEQGIYDILINVRDHYGNIDCFISENGMGVENEARFIKEDGMIHDDYRIKF
                     VQSHLKYVHQAIQEGANCLGYHMWTCMDNWSWTNAYKNRYGFISVDLAQDGKRTIKKS
                     GHWFKDVSDKNGFEA"
     gene            complement(2216..3187)
                     /locus_tag="IXT20_000997"
     CDS             complement(2216..3187)
                     /locus_tag="IXT20_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAQ4859464.1"
                     /translation="MASIRDVAKMAGVSVGTVSRYLNGQQLKEKNMKKIAEAISALDY
                     KENIIAKGLKNNRSFSIGLLINGISSRFGSEVVSGIERVAEENGYSLLLSGFADQPEK
                     IEQKIEYLMKHVIDGLIVFLSEEEWSGFEMLTKMSIPVIALNCPEGPAGIDTILVNDR
                     ESVAKVIAHHGEQGHKKIGFIAATQTDYVARERLAGAKEAVQNDPSIQLEVFTGDYSR
                     KSGYYGAKELLEKGVTAIFVSNYNMSIGAIECFNQEGIRIGKDIAFSHYDYLDKNDLS
                     ILPKITITPPTDKIGEQAAEQLLKRIKKEEEENEKVIVMENIIHGIN"
     gene            3561..3986
                     /locus_tag="IXT20_000998"
     CDS             3561..3986
                     /locus_tag="IXT20_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4859465.1"
                     /translation="MKKLIVLFILSAGLLTLTGCNLFGGSDQSSDETTDSSVLKAEKA
                     DITNSVYTLKVRKDMDLSAGQLTFTDKKMEWTRTYQTQETDSSESSEEKTVLTDIKIT
                     TKDDEYIISGKENKKEATITFTKIGNYRLKDEDGNIYSL"
     gene            complement(4474..5175)
                     /locus_tag="IXT20_000999"
     CDS             complement(4474..5175)
                     /locus_tag="IXT20_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HAQ4859466.1"
                     /translation="MSVILISGTIGAGKSSLTDMLAKEIDSKPFYENVEDNEVLPLFY
                     SNPEQYAFLLQIFFLNKRFLAMKNALVNDDNVLDRSIYEDSLLFHLNADLGRVTDIEV
                     QQYDSLLDTMLNELDDVAPKKRPDLMVHIKVSLDTMLERIKKRGRDYEQLESDETLYT
                     YYETLNTRYNQWYEDFDVCPKIQVDGDKFDFVENPEDAKKVIHQIQEKLAELEGKPAG
                     KYFTDNQHAKGQELG"
     gene            complement(5191..5631)
                     /locus_tag="IXT20_001000"
     CDS             complement(5191..5631)
                     /locus_tag="IXT20_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deoxyribosyltransferase"
                     /protein_id="HAQ4859467.1"
                     /translation="MSQIYLAGPFFSEEQIDRVSRIEKALEENKTVTSFYSPRHHQES
                     NYELFSAGWAQEVYEKDMEELTNAEFVVAILDFEHQTIDPGTAYELGVATMLKKPMII
                     VQEETVPTNLMITQSLHTYLKSDQAVREYDFETLPVETYVGEYL"
     gene            complement(5631..6206)
                     /locus_tag="IXT20_001001"
     CDS             complement(5631..6206)
                     /locus_tag="IXT20_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HAQ4859468.1"
                     /translation="MKLTVFCGSRFGNKESYKFIAQTLGKYMAQEEIELVYGGSYSGI
                     MGVISKTVLENNGKVTGIYPNGLFETELPGKDVTTFIPTETIDERKVLLFEKGDAVLV
                     FPGGLGTLEEFSQLLSWIAIGLTPDKPIGILNIGGYYSGLQQLLETFAKEDFMDKKWL
                     DQVIFSNNPLELVNELRAVSIKNQLLLKEAK"
     gene            complement(7053..7532)
                     /locus_tag="IXT20_001002"
                     /pseudo
     CDS             complement(7053..7532)
                     /locus_tag="IXT20_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015510350.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
     gene            complement(7691..8392)
                     /locus_tag="IXT20_001003"
     CDS             complement(7691..8392)
                     /locus_tag="IXT20_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ4859469.1"
                     /translation="MKRTRVLYLEVADKIKEDIFSGKYPVGSMLPTESELEELFQVSK
                     ITVRKAIELLASDEYVAKKSGRGTTVLSNRPYNRLSKAASFTQILENSRYEVRKETLD
                     LQLVHIKKEDPLFSFFGEQAVCFKRLYYLNNQPYIYFVHYLPQEMMQFSREEFGKESL
                     YRLLTKKGYTIDSFSDDFSAVFLTEEEQGILKTDAQIGIKRIRKSSTQIGEMIEYSEA
                     VYHTELHPYHIDYET"
     gene            complement(8500..9909)
                     /locus_tag="IXT20_001004"
     CDS             complement(8500..9909)
                     /locus_tag="IXT20_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HAQ4859470.1"
                     /translation="MEQLLPNQLEGAYDRDGKGLSVADAMPGGKQRFAIIGSEEFDWT
                     IDQEKYIYPNHRGIDHYDRFKEDLALFAEMGFKCYRFSIAWTRIFPNGDEGTPNEAGL
                     EFYDQLIDECLKYDIEPVITISHYEMPLHLAKEYGGWKNRKLIDFYERFAQTVLERYS
                     SKVKYWMTFNEINSAFHFPALSQGLVKSNGAGEYQNIFQAWHNQFVASSKAVKIGHEL
                     RSDIQIGCMIIYATTYSIDANPVNQAATMIQNQEFNFFCTDVQVRGEYPAYTARTHKK
                     YGVDPEKLEQTEKDFQLLKEYPVDYIGFSYYMSTAINETDPQAATSEGNLLGGVKNPF
                     LEASEWGWQIDPEGLRIALNELYNRYQKPLFIVENGLGAIDHMEEDGTIIDDYRIDYL
                     RRHIEAMANAVADGVDLMGYTPWGCIDLVSASTGEMSKRYGFIYVDLDDEGNGTLNRS
                     KKKSFDWYKEVIASNGVNL"
     gene            complement(9957..11822)
                     /locus_tag="IXT20_001005"
     CDS             complement(9957..11822)
                     /locus_tag="IXT20_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ4859471.1"
                     /translation="MDNQAVGRRVWEAVGGQKNVKSLVHCATRLRFRLKDESLADTQK
                     LKEDPDVIQVVQSGGQYQVVIGSNVADVYQSIVDEEGLAVSEDGKEESKNILNRLIDI
                     ISSIFTPFLGAMAAAGVLKGFLSLATVMGWLTADSGTYQILFAAADGVFTFLPVMLAF
                     TAAKKFKANQFLAVAIAMALVYPAITAVAGAGEAISFFGIPVILSPSGYTSSVIPIIL
                     AVWVQSKLEPFVKKVIPQFLQMILVPLVVLVVMVPLTFLALGPIGTVAGNALGGLFNS
                     IYGFSPIVAGLIMGSLWQVFVMFGMHWGFVPIMFLNIEQYGFDVLMPMLLPAILAQGG
                     AALAVALRTKDTKLRALGISSTVTSLFGITEPTVYGVTLPLKKPFIAACISGGIGGAI
                     IGFSGVKAFSSSLVSLLTIPTFINTVDGVESNVTVAVIATGIAFVLAFVGTLILGFDE
                     QTQDNQLENKHANAGEPITSARHTLKSPLTGKVLPLSEVPDQVFSSGVMGKGIAIDPE
                     VGELVAPADGEITTIFPTGHAVGITTTDGAEILIHIGMDTVELNGNGFEILVKQGDLV
                     KAGDLLIRFDIEAIRAAGYSVITPVVITNTDAFADILELDQKEIIANEDVLAIVK"
     gene            complement(11943..12794)
                     /locus_tag="IXT20_001006"
     CDS             complement(11943..12794)
                     /locus_tag="IXT20_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ4859472.1"
                     /translation="MKIDKILNNNVVISKNGFGEEVVCMGKGLAFQKKIGDEISPEAV
                     QKEFVLRDSFAKNQFQQLMADIPAEEIEWVKQVVELAEDKLKVEFSPNIYLTLTDHLH
                     YAITRAKENIQLPNPLLFETKKFYPKEYQAAKEALLFVKQKTGVELSEDEAGFIAFHF
                     VNSQQGNEDMQVTMTATTMVRDILSIISKFFGIVFDEESLNYQRIITHLQFFAQRYLK
                     GEGSDEQDEFLYALVQGKYPKAFHCVERINEYLRQTQKQEMGIAEQIYLTIHIQRVVS
                     EKKSIQS"
     gene            complement(13104..13817)
                     /locus_tag="IXT20_001007"
     CDS             complement(13104..13817)
                     /locus_tag="IXT20_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HAQ4859473.1"
                     /translation="MKKKGKAKRWLVNILLFLLLLVGLALIFNEQIKDYFVRETGDKY
                     AIANVTKEDLKKNNDKDVSFDFDAVEPMTTEGVMRSQMRGTDLPVIASIAVPSVSINL
                     PVFKGLDNTSLLYGAGTLSPDQEMGKGNYALASHRATNPELLFTPLENLEMGAKIYLT
                     DLENVYTYKTFFKEKVAPTDTQLLNEVEGKEIVTLITCGDMDAVTRLVVQGELESVTS
                     IKDATDDMRSAFNLETKTF"
     gene            complement(13885..14211)
                     /locus_tag="IXT20_001008"
     CDS             complement(13885..14211)
                     /locus_tag="IXT20_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HAQ4859474.1"
                     /translation="MNSMEKVNQFRDERNWRQFHNEKDLAISISLEASELLELFQWKT
                     PEEVKETQLERIKEELADVLIYSYMMADNLHLDLDEIIEEKLIKNNLKYPVEKSAGVR
                     KKYTEL"
     gene            complement(14334..14561)
                     /locus_tag="IXT20_001009"
     CDS             complement(14334..14561)
                     /locus_tag="IXT20_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HAQ4859475.1"
                     /translation="MLTQQLRELEQDESIHRKAYNQVPPKVEYSLTEEGKSLREILVA
                     LSVWEEERIEKQKASREEDTLLHTHGGYLNY"
     gene            complement(14883..16136)
                     /locus_tag="IXT20_001010"
     CDS             complement(14883..16136)
                     /locus_tag="IXT20_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HAQ4859476.1"
                     /translation="MVATVTLYLTFTLILDLGIYLFFSKQLLVKNSFGCIALMVVTLL
                     LPIPKVSFPYLVGLLLMFLFYFTTKNLFISCLISNLFFAITGLSFFLVFDLMDYLGKR
                     HLGTASFFALLIAFIFYLLVKSIDKQTRFLNLLFTYPTEHYFGGICILLSWGVIYLVL
                     GSVHFRSSNYLLLSLLSIVISFFSLFFSIMLISFQVKERQHQESLQLYQINEEYYHHL
                     EEFKHDYKSLLFSLKITLQENKKETLEFLEILEDHSAAIFEKPQIQQLTNIVSPAVRG
                     VFLKFLEKAESEKIPIQVVIPVEVKTIPIDLVVFIRCLSIFISNAFDHRVADQSPITI
                     NLLEEKEGSRFSIANKANPTNLTLSEMVKRGKTSKKNGGLGLYSAKKMLDVYENAELK
                     IEFSKQNHYFFVEMYLASRKSKSAY"
     gene            16197..16936
                     /locus_tag="IXT20_001011"
                     /pseudo
     CDS             16197..16936
                     /locus_tag="IXT20_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342003.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
     gene            complement(17022..17780)
                     /locus_tag="IXT20_001012"
     CDS             complement(17022..17780)
                     /locus_tag="IXT20_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HAQ4859477.1"
                     /translation="MKKTIALSTVLAGSLLLCTQSVFAENKSSATTEGTISVTKPDAA
                     NPEDVVPVPPVDPNDPDTPFTPDEPNQGTSGLLTIDQAPDLDFGTIKLGTAKTVYAAL
                     QSGTDSTGAQTQVQNFVQVTDKSGNYAGWKLSVKRTEFIGTTSDTNKLTGSKVKFKNA
                     VIRNSATNSSGMPSTVSGTGAEGIEIPADQAVDLVTAGANEGIGTWIYSLGENVEQGR
                     QSVELFVPVSNYAVDNYVSTFTWSLTDAPTEEEA"
     gene            complement(17777..18805)
                     /locus_tag="IXT20_001013"
                     /pseudo
     CDS             complement(17777..18805)
                     /locus_tag="IXT20_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628085.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
     gene            complement(18825..21992)
                     /locus_tag="IXT20_001014"
     CDS             complement(18825..21992)
                     /locus_tag="IXT20_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4859478.1"
                     /translation="MNQCVKKLIASILFVFPLLMVTSVFADVEKTNESTQSENQSFDK
                     TTFSEESITSETTESQSEASTNKRQEENKTSETSEVAEQTSDSSEAALARAVSADGYS
                     EAATTEELAQLLADESVTKIRLIQPLTLDRELEIKRDIVIDFGGFAHNFGTHHIYINE
                     IPQSIEFQNFKGTAAHPGGLTPNIDGNAIIIAYMTDFWGSRYHFTGEIKFTGTLDLYD
                     GSKLGLIYAPRATVTLDGVSGVLDVQPVKEGLNAAPGKAYFCRSYQLNVINGSQLYGP
                     YLGKFYGFLDSGDETGSGKTAPGIHILSGSKVSLDYDRADGVSDEGEAVDTLPGNVMF
                     EVSGSGSEFSVNTKINITNDNNRGIIQMRGSGSKVTVSNGGKITINTATTGGFRLQGD
                     SSQIHVSSGGQISVKMAGDDDQPGNNGMRFVGKGLSLIVEGAGSVIDIDKQSGRSSAV
                     RFENGTQKLTVTNGGSLRVRNTGDGKSYVNRLNQGNQAIQFYDASGFLESPGSADFTV
                     TGEKSNIDIRADSGATVDTTGDIDLNFTAGEKTYLVMVGKTGEDKTGIISGDVLNVDL
                     ESPTYFDFQNKRIGSETNGIGGWVFQGNGDSSLKIAHSEIALWRKTGATFDNFEKDPD
                     YFSDMTDLSINGTDLGNFVSSPSAGLTNEFAQTGAGMKNYNRISANNQLPMIESLRVP
                     TNADKKIYGHVVVPEGVEAKPRDAWTGEVEVTLRITYADTTKSSVEITALTQGQTDEN
                     EGAGYNPWGEGEQGGLFEVQVPNDERLKTEDQVQVVAAKKVRGGQEVTELDSAKTTVD
                     VIPPEPANLSTTLINAASRTITGTGEAGALVSLKKNQAVLSETTIDDTGEFVLSIPEN
                     QLFAGDVLEIVLNDQAGEATAKGVQNKPSTNDEIGNHNPSDTPVVYHDAPPFAPATKI
                     VVEGGLSFLAPSKLDFGQIKATGLTQEAFGQMDIQEKLWIYDQRETKNPWILTIKLSQ
                     PFTNDAGFSMQDVLYFKKGEEYQLVNEEELEIVRGQNSTNVQADYSEYLNNGRAFKLV
                     LTKENQIAGNYSAEITWTLADAPTE"
     gene            complement(22216..23100)
                     /locus_tag="IXT20_001015"
     CDS             complement(22216..23100)
                     /locus_tag="IXT20_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HAQ4859479.1"
                     /translation="MKKVLLVVNPSSGGEQAKEFEQLAIAKLESVFDEVVVLHTKKAG
                     DAKNFTREAATEGYHSVFVMGGDGTVNEGISGIAEQEHRPNFGFFPLGTVNDLARALG
                     IPLEPEEAINHFSIESVKPLDIGKINDDYFMNVVAIGSIPESINDVDAEKKTKFGKLA
                     YFMSGIKQLASTQSYSFHVEVDGKKEEIESSTLLIGLTNSVGGFETLLPNAKVDDGKL
                     HLVYLKDSSLIDTLKTLPDLLKGVDESTKNLAYLTCEQLVVSLADEEELTTNVDGDEG
                     DKLPVTIKVLPSHLQVYC"
     gene            complement(23195..23632)
                     /locus_tag="IXT20_001016"
     CDS             complement(23195..23632)
                     /locus_tag="IXT20_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4859480.1"
                     /translation="MEWFCKFLLFLEVIHVYGFVFLTNKKAGSTMDHLYIKYIHQFSV
                     LVLGTLIIAGIVAFIIQGRDILTVHQEFFSYAFGIPLIAIRSDNKKWKKRYYILLLFL
                     CFYLPPKIFTLLGIQCLSFSFISYIIVLIFLSFIVWMFYLVKK"
     gene            complement(23849..24760)
                     /locus_tag="IXT20_001017"
     CDS             complement(23849..24760)
                     /locus_tag="IXT20_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAQ4859481.1"
                     /translation="MKKRFLALAIVLGTGLLSGCTNAGEKTAVSYKGGTISEQEVMDS
                     LKKMQGADSAVQQLIVYQVFEDKYGDDVSTKEIDSQYDQTKKQLGDSFDSQLKSAGYT
                     EQTFKDSIKQSLAFQEGLKKHIKLTDEDLKTAWESFHPEVEAQIIQVASEDDAKDVKK
                     AADKGDDFSKLAKDKSTDTTTKEDGGKVKFDSTTTTVPAEVKEAAFKLKDGQVSDVIT
                     STNASTYTTEYYVVKMVKNQNKGNDMDKYKKELKEIATDTKLSDSTFQNKVIGEVLKD
                     ANVKIKDKDFENVLSTFTSDSSTASSK"
     gene            complement(25217..25645)
                     /locus_tag="IXT20_001018"
     CDS             complement(25217..25645)
                     /locus_tag="IXT20_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4859482.1"
                     /translation="MKKAVIICALGIGFVGGNAMSSEVEANSVHGEIYYKSLKPGEIH
                     FDAKGNIIKSNLPVDREISRTAINHSSGRWVYYSDTTCTRKTGHSNHYSRVYNRHGAK
                     AKVGNSIRSANATKGVWAYASATGNRTDTFSCWYNPTGWY"
     gene            complement(25673..26311)
                     /locus_tag="IXT20_001019"
     CDS             complement(25673..26311)
                     /locus_tag="IXT20_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ4859483.1"
                     /translation="MEPLIVIKGAKKIIRNKTILHLDRFTADAGDLVTVVGESGAGKS
                     TLLNILGLNDQFSSGQFYLFDQETKQLTGREKRLIKRNTISFLFQDFGLIEYETIDFN
                     LDIGLKFSNLNVSDKRTAKYEALKKVNINKKLSTPIYTLSGGEKQRIACARVLLKNSK
                     IILADEPTGSLDKRNKDKVVEILSQQASQGTLVIVVTHDPYLTKIGNKKIML"
     gene            complement(26315..28351)
                     /locus_tag="IXT20_001020"
     CDS             complement(26315..28351)
                     /locus_tag="IXT20_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1430 domain-containing protein"
                     /protein_id="HAQ4859484.1"
                     /translation="MIIKKKFTVLLTIIVTGIMSGFALVSINKQVLYRTNEFFHQTDT
                     YTPIRLPQHFIGQEEYEQLIMVFENVSSETELSYMKKTVNESNEVDTSWNFKIQPKLE
                     MTLYYQGDVKSSHEKVPYSNTSFRVTSIEESIKIKDFEGEFFLMTREKEAYERVISLF
                     TDRFNQAFHTDYATNIFEDFENSYSYSVISNTDVYNYRVYLTIGLILLFLLLSSFFLL
                     MNKEISLLLNNDFSLHQVLWCLLGKKKIIITFIIAGIIGGFIFMYYKEQFLYLFQMYV
                     FLFSGIYGISLISLLLVEKIASLREAFNYIQYILFTFGLLFSLLLTVANVELATIVFS
                     SSQLAVSHEPPKEIAGEDYHVFYPVTIGKNHVDFIYSNRFASAADQELYETLNKNGSI
                     LVNVSDYLNEENEQFGRGIKINLNYLKKFPLIDVSGRLIQITEKETHPVILIPERYRH
                     TDLKNDLAQITEYYQEISEKEPKFLFIKNNQPIYTFIPSIPWIEHYPVVEILTLKNSM
                     YLERNILSGEIYPPLKIKIGSLSKERLFELIEESQLRDNLQLSFPYTQTEEIAVKVLS
                     RSFHYLIQIFLLTFFSFFLLSYVMLCIYFVYNCRKIAIFRSSGYSLFETYKDFFMMNL
                     IKWGTTSVIFLFLIEREPKYLFNIFFFSFIDFILSVVFILSTEKKSQLLLMNGG"
     gene            28477..29142
                     /locus_tag="IXT20_001021"
     CDS             28477..29142
                     /locus_tag="IXT20_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ4859485.1"
                     /translation="MNTGEKIRTYRRNCKLTQKELGEKIGVSDKTISSWENSRTMPDL
                     EMLSLLHHTLGLPIDFPSIESATESIEPTLPDEPLVNKKSYSSKQPFSLKKTLSFFLL
                     VFCLVYLFLINYQYKNETIDRFNPFLKPQIGYTTLPVGYVDNSIERWVCNDIFGHGTW
                     LSFHAGEVEKEKNHAVIKHKGKHVMNVRLLTWEAIPSDYQEILSQDYDDALKRSPQEP
                     DRH"
     gene            complement(31077..34226)
                     /locus_tag="IXT20_001022"
     CDS             complement(31077..34226)
                     /locus_tag="IXT20_001022"
                     /EC_number="3.1.21.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HsdR family type I site-specific
                     deoxyribonuclease"
                     /protein_id="HAQ4859486.1"
                     /translation="MKISHREEAEVEEQLIRVLGEGHNQWTYRPDLKSEEDLWVNLRQ
                     KIISNNQAELNDFPLTDKEFETIKTELLLRTKTPFDAAKWLKGENGMARITIEREDPQ
                     LGSVSLILYSNQDIGGGISTYEVVHQIAKRGSNIEARDRRFDVTLLINGLPIVQIELK
                     QVTAKDGFYQAFNQIKKYAEEGMFRNNIFSTLQLFVVSNEQTTRYFANALPKDLHPKF
                     LFSWRTKDNKKVENLYEFCKQVLNIPDAHRLIADYTIVSEDQDNKILMVLHPYQVHAI
                     QALFIAANKHQSGYVWHATGSGKTLTSFVSTKLLARKTGIDRTIMLVDRKDLDNQTTT
                     EFTKFASEFNTGISSGNAKANSLIVGTGSAKELSETLLADANANVVIITTRQKLDAAL
                     KYAKKQEEKKGTNRFQKLTGQHIVFVVDECHRALSAENMEEIKKMFPKSTWFGFTGTP
                     IFPENRKQAKGQLARTTHDQYGEVLHTYTIKNALEDGSVLGFQVEHENTVEPTSLENK
                     IYRKLKEVETYAEYSSEQINRMIDQMEPVKKESYLDPAVFEADEHIQKVIHKMFRPDN
                     AYTKFDFRNGRPTKSAILTTSSIDMAKKYYRAIKEMTKEPDWLTKEFPNQLIREGRTM
                     EDPDFPRIAITYSLEENSRDSSAQQEEMQKIIEEYNQYYDTSWSLADIERYNGDINNR
                     LARKRAEFKQFGKQIDLAIVVDRLLTGFDAPTIQTLFVDRNLEYAGLIQAFSRTNRTY
                     PDKTKGLIVTFRKPATMEKNVEDATKLYSKAKEESGLIYPSYQESKKRFKQAYGKLKE
                     VSSMESIDEHTPLEIRIEYVKAFQELNRAYESLVTYDDYNDDMVKSPTLNNQIQLLEE
                     QIGVYETVKGSLIEEEPEKEGEDFSTIEFYSENTTKLYDIDSTYIDQLLGTYAANSSD
                     AREEIEKALAKLNKTEGVKEVYRQILNAFDVGTLDSNEDIFVIKRRYFTQASDDLIHV
                     FSNEWFVSEKELHASNLQYLPGEDPIPNMKAIINSKDYEGYKAKHPEAKPFKYPQEMK
                     RAWRKMLDDELIPLENELR"
     gene            complement(34239..35450)
                     /locus_tag="IXT20_001023"
     CDS             complement(34239..35450)
                     /locus_tag="IXT20_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HAQ4859487.1"
                     /translation="MSNDTQPEIRFPGFTDDWEQRKLGDFVVDYVEKTSVQNQFPMLT
                     SSQQKGIVLQEDYFANRQVTTENNIGYFVLPRGYFTFRSRSDNDVFVFNRNDIIDRGI
                     ISYFYPVFTLKSADSDFFLRRINNGIQRQLSIQAEGTGQHVLSLKKFKNIVAMFPSEE
                     EQQKIGTFFKQLDDTIALHQRKLDLLKETKKGFLQKMFPENGAKVPEIRFPGFTEDWE
                     ERKVFEISKVTYGGGTPKTNTKEFWNGNIPWIQSSDLEINRLFNVSPKKKITNEAVKK
                     SAAKIIPPNSIAIVTRVGVGKLALMPFEYATSQDFLSLSELQIDSYFGIFSLYSMLQK
                     ELKNIQGTSIKGMTKSDLLEKKVTIPKKYEEQQKIGNFFKQLDDMIALHQRKLDLLKE
                     TKKGFLQKMFV"
     gene            complement(35440..37035)
                     /locus_tag="IXT20_001024"
     CDS             complement(35440..37035)
                     /locus_tag="IXT20_001024"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HAQ4859488.1"
                     /translation="MGAELNQKLFSAADNLRSKMDASEYKNYLLGLIFYKYLSDRLLE
                     QVVLLADESLEEYDTVSKQTMLYRELLSDEESKEDLIATIVDILGYAIAPEYLFNVLA
                     DQAKQATFQLNDLNKAFVQLASTYNQFNGLFDDVDLQSKKLGTDEQQRNVTITEVIKK
                     LNDVDVLEHDGDVIGDAYEFLISQFASEAGKKAGEFYTPHMVSDMMAQIVTLDQKERR
                     FFSVFDPTMGSGSLMLNVRNYLTHPDNVKYHGQELNTTTYNLAKMNLILHGVDAEEMN
                     LRNGDTLNKDWPTDEPYTFDAVVMNPPYSANWSADTTFLDDSRFNRYGKLAPKSKADF
                     AFLLHGFYHLKETGTMAIVLPHGVLFRGAAEGVIRQKLLEDGSIYAVIGMPANLFFGT
                     SIPTTVIVLKKNRQTRDVLFIDASREFVKGKNQNKLSEENIQKILETYAERKDVEKYA
                     HLATFDEIKENDYNLNIPRYVDTFEEEEPIDMVHVGNDIKKIRQEQQVLEKKLLEAIS
                     SLQTTPENEAWLQGALEVFKHEQ"
     gene            complement(37544..38326)
                     /locus_tag="IXT20_001025"
     CDS             complement(37544..38326)
                     /locus_tag="IXT20_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4859489.1"
                     /translation="MENSSNSAEVNVSTSTSEVVKCSQETTSLPEELLGDWQINNKDA
                     GIEISDSKAVFRIGRSQVESKDYYVDVNNKMLLHVYFSFEYDSVKYIEVSIERTGYSE
                     FISKSVGILTMHTKDGQYISLGVKRVSGENNETEAVTEQTMDERIDSSKSNETEVDTG
                     KTIDERIDSFIETYTAIPPTWKLVNEDKGITINTDTEISTDAQSLSDLLANLTGISNA
                     VTEQVGKDIPLRLLENGKKDYSVVFKNGQVTDYNEVFKKVIE"
     gene            complement(38391..39227)
                     /locus_tag="IXT20_001026"
     CDS             complement(38391..39227)
                     /locus_tag="IXT20_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ4859490.1"
                     /translation="MRVIKTLNNSLILCLDKQGKEVILMGKGIGFQLKKGDLVPADKI
                     QKVFKLDSLSDALKKDYIKLFEAAPDDLIICVKRIIDYANAKFDFKLNKSLFFTLVDH
                     LKHAIERSQKGIVFQNRLLIEIQKYYPKEFEIGCYAIQLINKKMNVQLPEEEAGNIAF
                     HIVNAQSEQANMEETMLSVRALKDILAMLGYLFPEKKVDKDSMLYLRFVTHLQFFIAR
                     MIKKEELPEKDDFLLASVKQRFPKEYRAALKIKEYVQEEMGASANQDELLYLTLHISR
                     VY"
     gene            complement(39245..40654)
                     /locus_tag="IXT20_001027"
     CDS             complement(39245..40654)
                     /locus_tag="IXT20_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010757186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAQ4859491.1"
                     /translation="MQYKQLSAFPEKFLWGASISAYQAEGAYQEDGKQPSIIDKYQHT
                     EGVANFEVASDFYHHYKEDIQLFKELGLKAFRFSIAWTRIMDIENNKTNQKGISFYHQ
                     VIDECLNQGIEPIVTMYHFDLPYFLEEQGGWLNRATIDYFVDYVQVLLTEYGDKVNYW
                     LTINEQNTMILHPGAIGLPPSGELPSKKELFQINHHVLLAQARVIHLYHQLNLKGKIG
                     PAINLTAMYQATSSPEDAIAAHNWETLRGWSFLDVAVRGKYNYLFENYLNDRGLYPKI
                     EAEDQAILSSGKPDFIAINYYSTATIAASKNDGSDVSARAGDQQIMLGEEGVYRAAEN
                     PYVDKTPYGWVVDPTGLRLTLRKLYDRYDLPILITENGYGAPDIVEEDRRINDQDRID
                     YLAKHILAIQAALTDGVDVFGYLPWSAIDVVSTHQGFNKRYGFIYVDRTDENLKELKR
                     IKKESFFWYQKVIARNGLA"
     gene            complement(40668..42491)
                     /locus_tag="IXT20_001028"
     CDS             complement(40668..42491)
                     /locus_tag="IXT20_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ4859492.1"
                     /translation="MDYREVGRNVLQQVGGKENVVSLTHCATRLRFELKDKRKADTKA
                     LEKTLGVISVVDSGGQYQVVIGNEVQTAFKEIQKEVGTSIKKGPSSDNQESIISRLIS
                     VISTTFTPMIPAITGAGMVKAILAVLTLTGVLSDSSQTYIILNTIADAAFYFMPVLLA
                     YGAAIKFDVNPILAITVAGILLHPNISQLFSTGDAVSFLGIPVLAADYAGSVLPIIFT
                     VWIMSYVEPFADRISPSFIKFFTKPMLVFLIMGPLALVVIGPFGTLLNDLIAMGAEWI
                     NGKAAWLIPFLMGGLQPFLVVTGTAWAMTPIATMQLSSTGSEMINGPGMLASNIAQSG
                     ATFAVAAKTKNKELKQLAVSTGITALMGITEPSLYGVTLKLKKPLVASMIGGAIAGLY
                     AGLSGLVRYAFVSPGLAALPAFIGENPMNIVHAIITCVIAFVATFIIAYLIGFDDTSD
                     ESEQTAIAEAVIVENPVKGTVCPISEVNDAVFSKGLLGKGVAIHPEENSIYSPVEGEV
                     ITILESKHAIGLRSKEGIELLIHVGIDTVNLKGEPFEVFVKAGDQVSAGQLLINADFA
                     AIRNAGYDDTVIVVVTNSENYAGISEASGSLKEHEPLMQIS"
BASE COUNT        12495 a         8855 c         6937 g        14476 t
ORIGIN      
        1 tttttcattg tccataggat tgatattgtc cttgtgatga ctcattcgta ataattgagg
       61 cttgattttc ttcacttccc gttatcgttt gtccactgcc agaatctgaa ggacttgttc
      121 ctgtcgactc gtttttttga gaagagacag ttgttttctt gacttgttca tttgtctttt
      181 tccattccgc taaacgatcg gcgaccactt ttttgtctac tttttcagaa taagcggtga
      241 tcacttttcc atttttcaaa cgggctactt tgtcgccatc atgagtcaaa agcaaaacgg
      301 tcaaatgctt gtctttaaat tgatattgtc tgagagaaat cgcactaagt acgtcatcga
      361 tattaataaa ttgattttct tcttttcctt ttggcaaata gatgtccaat tcgccttttt
      421 cgtaattggc agttatttta tactccgccc accaataagt tgagaattct tttttgaaat
      481 tatctactga ttctttgctt gctacgattt tttctgtttc tttctgagca agatctgctg
      541 tcatttttcc tgaaaatgct gcggataaaa taagcagtaa gaaaaaaccg aataaccaaa
      601 agagaagaaa cggtcttttt ttcctcatgc tttcctccac tcctatttaa ttttgactat
      661 tctattatat acaaaaaatg aaatagaaag acaaaaaagt tgaaaaagat cgtaaataat
      721 tgaacttctt tttcacaaaa tggtaagaat ggagaatttc acgcatttgc tctcaactat
      781 tctttcttaa gcctcaaatc cgtttttatc tgatacgtct ttgaaccagt gacccgattt
      841 tttgattgtt ctttttccat cttgtgctaa gtctactgaa ataaagccat atcgattctt
      901 atatgcattg gtccatgacc agttatccat acatgtccac atatgatagc ctaaacaatt
      961 ggcaccttct tgaattgctt gatggacata tttcagatga gattggacaa atttgatccg
     1021 ataatcatcg tggatcatgc catcttcttt gataaatcgt gcttcgtttt ctacacccat
     1081 accgttttca gaaataaagc agtcgatatt tccataatga tctcgtacat tgatcaaaat
     1141 atcatagatt ccttgttcat agatctccca tccgcgataa ggattcattt tacgaccagg
     1201 catttcataa ttgtcgaaat aatcctcagg cattggaccg tttttagtat cgattggtgt
     1261 ttcttttgct tttacccggc gtggctggta ataattgata cctaaaagat ctactgtatt
     1321 ttctttgatg accgtcaaat cctctggatg gatcacaggc atgagatcaa gttcttttac
     1381 tatctcaatc aattcacttg gaaactcgcc ttttacagct ggatcaagga atgaacgatt
     1441 gaagaacgca tctgctattt gcgctgcttt aacatcttca gggtttgatt cgtctcttgg
     1501 atagcttggt gtcagattca agatgatacc aataacacca ccttgctcca tttcatggta
     1561 agatttgatg gctaacgctg aggccaacgc ttcatgatag ccaacttgga tcgctttttt
     1621 catatctgac tcttctggat aatgccatcg atataaataa cctgcttcga ctggaacgat
     1681 tggttcatta tgagtaaacc atttttttac tttcgaaccg aacaattcaa aacaactttt
     1741 ggcatatttt tcataggctt caaccgtttc tcggttcaac catccgcctt tttcttgcag
     1801 atacatcggc atatcgaaat gatacagatt cacaaagggt tcgataccgt tttcaatcaa
     1861 ctcatcaaaa taacgatgat agaaacgaac ggcttcttcg ttgattgttc cagattcatc
     1921 aggtatcaag cggctccatt ggatagatgt tcgaaatgaa ttatgcccag tctgtttcat
     1981 caactgtaca tcctcttgat acttcgtata aacttgtgaa gttttttcag gtcctacttg
     2041 gtcaaagaac ttttgcggtt cttttttgta ccaataatca aaaatgttct ctccttttcc
     2101 atcacctgaa atccttcctt ctgtttgtgg gccacttgct gcagaacccc accaaaaatt
     2161 tgctggaaat tgatattttt tcatcaaagc acctcttttt cttttttagt tcgttttagt
     2221 tgattccatg tatgatattt tccatcacaa ttactttttc attttcttcc tcttcttttt
     2281 tgattcgttt caacagctgt tctgcggctt gttccccgat tttatctgtt ggtggtgtaa
     2341 tcgtgatttt cggaaggatg ctcaaatcgt ttttatccaa ataatcataa tgactaaacg
     2401 caatatcctt cccgattctg ataccttctt gattgaaaca ttcgatagca ccgatcgaca
     2461 tattataatt gctgacaaaa atcgctgtta ctcctttttc taataattct tttgccccat
     2521 aatagccact ttttctagaa tagtctcctg taaagacctc taattggatc gatggatcat
     2581 tttgtaccgc ttcttttgct cctgctaaac gttctcttgc tacgtagtct gtttgagtgg
     2641 cagcaataaa tccgattttt ttatgtcctt gttcaccatg atgagcgatc acttttgcta
     2701 cactttctcg atcattcact aatattgtat caatacctgc tggtccttct gggcaattca
     2761 atgcgataac tggaatactc atttttgtga gcatttcaaa tccagaccac tcttcttcgg
     2821 ataagaagac aatcagccca tcgatcacat gcttcatcag gtattcaatt ttttgttcaa
     2881 ttttttctgg ctgatcggca aacccgctta ataataagct atacccattt tcttcagcta
     2941 ctctttcgat ccctgagaca acttccgacc caaaacggct ggaaatcccg tttatcaaaa
     3001 gtccaataga gaagcttcga ttattcttca accctttagc aatgatgttt tctttatagt
     3061 ccaacgctga aatcgcttct gctatttttt tcatgttctt ttccttcagt tgctgaccat
     3121 tcaagtaacg ggaaactgtt cccactgaaa caccagccat cttagccacg tcacggatcg
     3181 atgccataca ttctcactcc tgccagttac atttgatttc tctttcttgt tgttcgatta
     3241 tgatgaccgc tttctttgca tagtgttgct tgtcatcaat atccatataa ctaaagtctt
     3301 cagtagagca acttcctctc actattaatt atacttaaac aagtatttga accggttcat
     3361 ttttaatata gcattttttt aaaagattgc aacacttttt taaaacgctt tcctaaaatg
     3421 gcgaattttt tgccttttta aaattttatt ctaaaattta ctctatttgt gggtgttttg
     3481 tgcgtttttc tcttccatcc gtttgaagtt ttactttcca atggtataat ttctttatcc
     3541 tatcaaatgg gggaagtatt ttgaagaaat taatcgtact atttattttg tctgcgggac
     3601 ttttgacatt gactggctgt aatttgtttg gcggctctga ccaatcctcg gatgaaacaa
     3661 ccgattcttc tgtcttaaaa gcggaaaaag cagatatcac taacagtgta tatacgctaa
     3721 aagtacgtaa agatatggat ttatccgcgg gacagttgac atttacagat aaaaaaatgg
     3781 aatggacacg cacgtatcag acacaagaaa ctgattcttc tgaatcatct gaagaaaaaa
     3841 cagtgctgac agacatcaag attactacca aagatgacga atatattatt tcaggaaaag
     3901 aaaataaaaa agaagcaacc atcacattta ccaaaatcgg taattaccgt ttgaaggatg
     3961 aggatggcaa catctacagt ctatagtgcc gtctgttgga tcttcttttg tttctcccct
     4021 tcttttctgc tatagtaaag aagcaagatg attgtaaagg agacgaaatg gatgatcgat
     4081 agtcctgatt attgtacatg taataacaac aaaaatatcc atacagaatt taatgaccaa
     4141 ggaagcttga gcatatgcga caactgtcac aaactagtca aagattcatt taaaacattt
     4201 gaatcttatc ctgttcaaaa ttatgtaaaa agtagaagaa gataaacaaa tccaagcaaa
     4261 actcactatg gctgattttt atgaacttca aactcagctt cttcctttga tcaagcttca
     4321 tcagtaaaac aaacagtgga tcatttcagt ttacaaatca aagagtaaag atttatttat
     4381 ttcaaatatt tgaatataaa ataaccaaac gatagaaagg cttattctac atcacgtagt
     4441 aatttttctt tcccgtcgtt tggtttttta tttttagcct aattcttgac cttttgcatg
     4501 ctggttatcc gtaaaatatt ttccagcagg ctttccttcc aattcagcta gtttttcttg
     4561 gatctgatgg atcacctttt ttgcatcttc tggattttct acaaaatcga atttatcccc
     4621 atccacttgt attttaggac atacatcaaa atcttcatac cattgattat agcgggtgtt
     4681 caacgtttca taataggtat ataacgtctc atcgctttct agctgttcat agtcacgccc
     4741 acgttttttg atacgttcta gcatcgtgtc caaagaaact ttgatatgga ccatcaaatc
     4801 aggccgtttt tttggagcaa catcatctaa ctcgtttagc atcgtatcta ataagctatc
     4861 gtactgctgt acttcgatat ccgttacccg acctaaatca gcgtttagat ggaacaacag
     4921 gctatcttca tagatggatc ggtctagcac attgtcatcg ttcactaaag catttttcat
     4981 tgctaaaaat cgcttgttca aaaagaagat ttgcaataaa aaagcgtact gctcaggatt
     5041 tgaataaaag agcggcaata cttcgttatc ttcgacattt tcataaaatg gtttagaatc
     5101 aatctctttt gcaagcatat ccgtcaaact gctcttacct gcaccaatcg ttcctgaaat
     5161 caaaatcact gacatacaac ccttcctctt ctataagtac tcgcctacgt aagtttcaac
     5221 aggcaatgtt tcaaaatcat attcacgtac agcttggtct gattttaaat acgtatggag
     5281 actttgtgta atcatcaagt tcgtaggtac cgtctcttct tgtacaatga tcatcggttt
     5341 tttcaacata gttgccacac ctaattcgta agccgttcct ggatcgattg tttgatgctc
     5401 aaagtcaagg atagcaacaa caaattctgc atttgtcagt tcttccatat ctttttcgta
     5461 tacttcctgc gcccatccag cgctgaatag ttcataattg ctttcttggt gatgacgtgg
     5521 agagtagaaa cttgtgactg ttttgttttc ctccaacgct ttttcgatac gacttactcg
     5581 atcgatttgc tcctctgaaa aaaatggtcc tgctaaatat atttgactca ttattttgcc
     5641 tccttcaata ataactgatt tttaatagat actgctcgta attcattgac cagttccaat
     5701 ggattgttgg aaaagattac ttgatccaac cattttttat ccataaaatc ctcttttgca
     5761 aaagtttcta ataattgttg caagccgctg taataaccgc caatattcaa tattccgatt
     5821 ggcttgtcgg gcgtcagacc gattgctatc caagaaagaa gttgtgagaa ttcctccaat
     5881 gtacctagac ctccagggaa aacgaggaca gcatctccct tttcaaataa taataccttt
     5941 ctttcatcga tcgtttctgt aggaataaat gtagtaacat cttttccagg caattcagtt
     6001 tcaaaaagcc cattcggata gatacctgtg acttttccgt tattttccag tactgtttta
     6061 ctaatcactc ccataatccc ggagtatgaa ccaccgtaaa ccagttcgat ttcttcttgt
     6121 gccatgtatt ttcctaacgt ttgcgcaata aatttgtaac tttctttatt accaaaacgg
     6181 gaaccgcaaa aaacagtaag tttcataaaa aaacactccc taactcaaaa gtctatcttc
     6241 attctacacg tgttttttta aaataaaaga ctagattttg tagtttttta gtggtatata
     6301 ggatagacac cactatatat agtatttaga aaaaaacatt tttcagctat tttatagata
     6361 aaacaaaaat aaataacgtg ttttcatcga tgtgaaatga cttacttata gaagatactg
     6421 ggatttcgtc ctatatgcca ataacgtgta ttattttaaa caacaaatac ttgtttcttt
     6481 tattaacgaa aaaatcattt tgttctcgtg ttatgtttca ctgataaatg atgtttctct
     6541 aaataaaaaa gccgacgaac taaacgatca aaatttgatc atttttgttc atcagcttct
     6601 cctaaaaatc tttttgttaa cgataaatag ctggatttgt aaaaatatca cttgaatcgt
     6661 cactgctgga agaaaattca gtgataaccg cacctgttgc gcctgcctgg aactagtgtt
     6721 tgatattggg ctcgaacgtg tactgatctc tagcatgaag aataatttct ttttttactg
     6781 tataataact ttctttcctt tttggaatag tacaggaaag tttttttgtt tcctttcctt
     6841 cgacataagg tataccagac cctttcgaca acgaaaggtt ctcatttttc cttcatcgtc
     6901 atttcaagga gggtgccgat gctctggaca gtttcgatcg ctcctaaacc aaaatctgta
     6961 tattcgattc tttctaactc tgattctttc ataccattcc tgcctcaagg tacatctatt
     7021 gtactttttt cttgtaatcc attctgcctc tcctaactaa ttgtcaaatc atcccaagat
     7081 atgtcttctt cctctttttc ttcttggatg atttctgcat tcggatctac tggttttttc
     7141 aataataaaa tggttattcc tgtgacaaaa gaaccagcta acaatgcacc aatgaatgcc
     7201 catggtctgg tcatcgtagc gactgcaaac attcccgatg ccggaatacg gctatctgcg
     7261 ccccaaaaca tgcttaatcc gccacctact gtcccaccga ctcccgttgc tatgacattg
     7321 cgtgccaagt cgttcaatac gatagggaaa catccctctg tgatctgaca gatccccata
     7381 ggaaaagcgg tcttcaatgt atcgatttct tgacgattga gtatattttt acgaatgatt
     7441 ttccctaaga aataagcgat cgtcaagccg aatggggtta ccatcgaagc taaaatcaat
     7501 acagtgattg gttcatagat tccttctgaa aatcgtgcca caccccctta tccagaataa
     7561 atggtaatcc agaaacagcc gtgtatctgc ggtaataagc ctaaccaaac gaaaaatatg
     7621 agaagctatt tttgtttggc tgttcttatt acttgatgca gggcagcttt ttcacaacct
     7681 ctttttttat ttatgtttca taatcaatat ggtaaggatg cagctccgta tggtagaccg
     7741 cctccgaata ttcaatcatt tccccaattt gcgtagacga tttccgtatc cgtttgatgc
     7801 cgatctgtgc gtctgttttc agaattcctt gttcttcttc tgttaaaaat actgccgaga
     7861 aatcatcaga aaatgagtca atagtatatc cttttttcgt cagcaaacga tacaaagatt
     7921 ctttgccaaa ttcttctctg ctgaattgca tcatttcttg tggaagataa tgaacaaaat
     7981 aaatataagg ctgattgttc aaataatata agcgcttaaa acaaacagct tgctcaccga
     8041 aaaaggaaaa caaaggatct tccttcttta tatggaccaa ttgcaaatcc agcgtttctt
     8101 tccggacttc atatcgggag ttctccaaga tttgcgtaaa gcttgccgct ttggataatc
     8161 gattgtaagg acgattgctt agaaccgtcg tcccacgacc acttttcttt gcgacatatt
     8221 catctgatgc cagcagttcg attgcctttc ggacagtgat tttgctgact tgaaacagtt
     8281 cttctaattc actttctgtc ggcaacatcg aaccgactgg atattttcct gagaaaatat
     8341 cttctttaat tttatctgcg acttccaagt atagtacacg tgtccttttc ataacgtcac
     8401 cctttgcttt actattacta aaagaatagc acataaaaaa taaaaaaaga acacaaatca
     8461 catgtgagaa gtgtgttctt tttcattcag ctgctttaat tacaagttta cgccatttga
     8521 ggcaattact tctttatacc aatcaaaaga ttttttcttt gatcgattca gtgtcccatt
     8581 tccttcatcg tctagatcca catagataaa gccgtaacgt ttactcattt ctcctgttga
     8641 tgcactgaca agatcgatac atccccaagg cgtatagccc atcaaatcta caccgtctgc
     8701 tactgcatta gccatcgctt cgatatgacg acgtaaataa tcgatccgat aatcatcaat
     8761 aatcgtgcca tcctcttcca tatgatcaat ggctccaaga ccgttttcaa caatgaataa
     8821 tggtttctgg taacgattgt ataattcatt caatgcgatt ctcagccctt ctggatcgat
     8881 ctgccagccc cattcacttg cctcaagaaa cggatttttt actcctccca gcaagttccc
     8941 ttctgaagtt gctgcttgtg gatcagtctc attgattgcc gtagacatat aataactaaa
     9001 tccgatgtag tccactggat attcttttag caactggaag tctttttccg tttgctctaa
     9061 tttttccgga tcgactccat attttttatg ggtacgcgct gtgtatgcag ggtattctcc
     9121 acgtacttgg acatccgtgc agaaaaaatt gaattcttgg ttttggatca ttgttgctgc
     9181 ctgattgact ggattggcat cgatgctgta agttgttgca tagataatca tgcagccgat
     9241 ttggatatcc gaacgcaatt cgtggccgat ttttacggct ttgctacttg caacgaattg
     9301 attatgccat gcttggaaaa tattttggta ctctcctgct ccattgcttt ttactaatcc
     9361 ttgacttaaa gctggaaagt gaaaggccga attgatttca ttgaaagtca tccaatattt
     9421 gactttactg ctgtatctct ctaatactgt ctgagcaaat cgttcataga agtcgatcag
     9481 ttttcgattt ttccagccac cgtattcttt tgccaaatga agaggcattt cgtaatgaga
     9541 aatcgtgatc acaggttcaa tatcatattt taagcactca tcaatcagct gatcataaaa
     9601 ttctaatccc gcttcgttag gagtaccttc gtctccattt ggaaaaattc gtgtccaggc
     9661 aatcgaaaaa cgataacatt tgaatcccat ttccgcaaac aaggcaagat cttctttgaa
     9721 acgatcgtaa tgatcgattc ctcgatgatt cgggtaaata tatttttctt gatcaatcgt
     9781 ccagtcaaat tcttcactgc cgataatcgc aaagcgctgc ttacctccag gcatagcatc
     9841 tgcaacggat agtccttttc cgtctcgatc ataagcccct tctaattgat ttggcagcag
     9901 ttgctccacc ccataaaaat ttttctgaaa aacgtgattg catcctgatt tcctccttat
     9961 tttacaattg ccagaacatc ttcgtttgca ataatctctt tttggtctaa ttcaagtata
    10021 tcggcaaatg catcagtatt cgtaatgaca actggagtga ttacgctata gccagctgct
    10081 ctaattgctt cgatgtcaaa acgaatcaat aaatctcctg ctttgacgag atccccttgt
    10141 ttgactaaga tctcaaagcc attcccattt aattctactg tatccatccc gatatgaatc
    10201 aaaatctccg ctccgtcagt cgttgtgatc cctacagcat gtccagttgg aaaaatagtc
    10261 gtgatctctc cgtctgcagg tgcaactagt tctcctactt ctggatcaat agcgatacct
    10321 tttcccatca cgccagaaga aaaaacttgg tctggtactt cactcaatgg cagtacttta
    10381 cccgttaaag gactttttaa ggtatgccgt gctgaagtaa tcggttctcc tgcattcgca
    10441 tgtttgtttt ctaattgatt gtcttgtgtt tgttcgtcaa atcctaaaat caatgtacct
    10501 acgaatgcaa gaacaaaagc aattcctgta gctattactg ctactgtcac atttgactct
    10561 acaccatcca ccgtattaat gaaagtcgga atcgtcaata agctgaccaa actgctggag
    10621 aaagctttta ctccggaaaa tccgataata gctccaccaa tacctccaga aatacaagcg
    10681 gcaatgaatg gtttcttcaa aggtaatgtc acaccataca cagttggttc agtgattcca
    10741 aataacgaag tcactgtaga agaaatccct aatgctcgaa gtttcgtatc ttttgttcgc
    10801 aatgctacag ctaaagcagc cccaccttgt gccaagatgg ctggaagaag cattggcatc
    10861 aatacatcaa acccatattg ttcgatattc aaaaacatga tcggtacgaa tccccaatgc
    10921 atgccgaaca tcacgaatac ttgccataaa cttcccatga ttagacctgc tacgattgga
    10981 ctaaatccat aaatcgagtt gaagagaccg cctaaagcat taccggcaac cgtcccgatt
    11041 ggtcctaaag caagaaatgt taatggaacc atcactacta aaacgacaag cggaactaaa
    11101 atcatttgca gaaattgcgg aatcactttt tttacgaatg gctctaattt actttgaacc
    11161 catacggcta gaatgattgg tatgacagaa gaagtataac cagatggtga aagaatcact
    11221 gggatcccaa agaaactgat agcttctccc gcaccagcaa ctgctgtgat agctggatac
    11281 acaagtgcca tagcaattgc tactgctaaa aactggttgg ctttgaactt tttagctgct
    11341 gtaaaagcaa gcatgacagg taagaaggta aagacaccat ctgctgcagc aaacaatatt
    11401 tgataagtac cgctatcagc tgtcagccag cccatcaccg ttgccaatga taagaatcct
    11461 ttcaataccc cggcggctgc catcgcccct agaaaaggtg taaatatact agagataata
    11521 tcgatcagac gattcaaaat atttttggac tcttcttttc catcctccga aactgcaaga
    11581 ccttcctcat ccacgatcga ttgataaaca tctgctacat tactgccaat cactacttga
    11641 tactgaccgc cactttgtac gacttggatc acgtccgggt cttcttttaa cttttgcgta
    11701 tctgctaaag attcatcttt taatctgaat cgcaaccgcg tagcacaatg tactaaactt
    11761 ttgacattct tttgtcctcc taccgcttcc catacacgac gaccaactgc ttgattatcc
    11821 attgtttttt ccttctttct tttttattta aaaaataaaa acccagattg aaaaaaacag
    11881 tcacaaacat tcgtgaacct attttttcaa tccaggtttt gcctgccata cagtaacaat
    11941 ccttatgatt ggatgctttt tttctcactt actactcttt gaatatgaat cgttaaataa
    12001 atctgctctg cgatccccat ttcctgtttt tgcgtttgtc gaagatactc attgatccgt
    12061 tcgacacaat gaaacgcttt tggatacttt ccttgaacta atgcgtagag aaattcatct
    12121 tgctcgtcag atccttcccc tttgagataa cgctgggcaa aaaactgcag atgagtaatg
    12181 atacgctgat aattcaaaga ttcttcatcg aacacgatgc cgaagaattt actgatgata
    12241 ctcagtatat ctcttaccat tgtggtagcg gtcatcgtca cttgcatgtc ttcgtttcct
    12301 tgttggctat tcacaaaatg aaaggcaata aaaccggctt catcctcaga aagttccaca
    12361 cccgtctttt gtttcacgaa caaaagagcc tctttcgctg cttgatattc tttcggataa
    12421 aatttttttg tttcaaataa taatggatta ggcagttgga tattttcttt tgctcgtgtg
    12481 atcgcataat gaagatgatc ggtcaatgtc agataaatat taggcgaaaa ttcaactttt
    12541 agtttatctt cagccagttc gactacttgt ttgacccatt cgatctcctc tgccggaata
    12601 tcagccatca gctgctggaa ttggttttta gcaaaagaat ctcttaaaac aaattctttt
    12661 tggaccgctt ctggcgaaat ctcgtcccca atcttttttt gaaaagctag tccttttccc
    12721 atacaaacga cttcttcccc gaatccattt ttcgaaatga cgacattgtt attcaaaatt
    12781 ttatcaattt tcattttgct tcgccctctc aagaaaaata aaaaaaccta gaattcgcct
    12841 actgtttctt agctgaattc tggttttgcc tgccgtacag taacaatcca tttcttattc
    12901 aaaatcatca taccatcgaa tttttattta tgcaagcctt ttcatataaa aaataaaaga
    12961 actggaacac aagtctatct agaaaacaac caaatcagag atgcacctct ttgcgtttct
    13021 ctttttttca gcaaattggt ttttccttat cgattcatat cctttggctg tttttaatct
    13081 gtaacgactt atgtcccagt atcctaaaat gtttttgtct ctagattgaa agcagatctc
    13141 atatcatctg tcgcatcttt tatcgatgta acactttcca gttctccttg tacaactagt
    13201 cgcgtgacgg cgtccatgtc gccacaagta attagtgtga caatttcttt tccctcgact
    13261 tcattcagta attgggtatc tgtaggagca actttttctt taaaaaatgt tttgtatgta
    13321 tacacatttt ctaaatctgt caaatagatc ttggcaccca tctctaaatt ttctagcggt
    13381 gtaaacagca attcagggtt cgttgcacga tggcttgcta aggcataatt tccttttccc
    13441 atttcttgat ctggagataa tgttcctgct ccgtaaagta gtgacgtatt atccaagcct
    13501 ttgaaaaccg gtaaattgat tgaaactgaa gggactgcga tactagcaat cactggtaga
    13561 tctgttcctc tcatttgcga cctcatgacc ccttctgtcg tcattggttc taccgcatca
    13621 aagtcaaaac tgacatcttt atcgttattc tttttcagat cttcttttgt tacattcgct
    13681 atagcatatt tatctcctgt ctctctgaca aaataatcct tgatctgttc attgaaaatc
    13741 aaagctagtc ctactaataa caagaggaac aagaggatat tgactagcca tctttttgct
    13801 tttccttttt ttttcatttt ctctccctcc agactcattt tactgttttt cttttgctct
    13861 ttccaacagc cgaatgacac atttctacaa ctctgtgtat ttttttcgta cacctgcgct
    13921 tttttctaca ggatatttca aattattttt gatcaatttt tcttcaatga tctcatctag
    13981 atccaggtga aggttgtctg ccatcatgta tgaatagatc aatacatctg ccagttcttc
    14041 tttgattcgt tctaattgtg tttcttttac ttcttccggc gttttccatt gaaaaagttc
    14101 taacaattca gatgcttcca aagaaataga gatagccaaa tctttttcgt tatgaaattg
    14161 tcgccaatta cgttcatctc taaactggtt gactttttcc atactattca tttgcttgcc
    14221 ttcttccaat cacttcttac ttgattaatc ataccatttt tttacataaa aaatagatga
    14281 tgcttttaaa aaaactcctg ttttctttta tttaaaataa atgtcataga aaatcaataa
    14341 tttaggtagc caccatgtgt atgcagtagc gtgtcttctt cccttgaggc tttttgtttt
    14401 tcgatacgtt cttcttccca aacagacaac gctactagaa tctctcttaa tgattttcct
    14461 tcttcggtga gcgagtactc tactttaggc gggacttggt tataggcttt tcgatggata
    14521 ctttcatctt gttctaactc tcttaactgc tgggtcagca ttttttttgt gatcgtaggg
    14581 atttgcttgc ttctccacgc ctaaaataag cgatattgct cttcccttta tctggtgtaa
    14641 gcaaagcagt tagcattttg acgatcgtgg gttccgcttt tcacttctta tctacttagt
    14701 atcatgaagt atactttcgt actaatacta agtagcggaa gtgtcaacta ctttgtttaa
    14761 ctacatagca aaagaaacag aagggtgaca acggtcatca cgcttctgtt tcttttgaat
    14821 atgctgtgtt gttgtctcta tctccgtgta gactttcctc aaacttatcc tttgacagta
    14881 agtcagtagg ctgatttgct ctttctagat gccagataca tttctacaaa aaaatagtga
    14941 ttctgttttg aaaattcgat ttttagttct gcattttcgt aaacatccaa catttttttc
    15001 gcactgtata gacctaaccc gccatttttt ttagaggttt ttcctctttt gaccatctcc
    15061 gatagcgtca aattcgttgg atttgccttg ttcgctatac taaaacgtga tccctctttt
    15121 tcttccagca gattgatcgt aatcggactc tgatcggcta cacgatgatc gaatgcgtta
    15181 gaaataaaaa tagataaaca gcgaataaaa acaaccagat caatcgggat tgttttcact
    15241 tcgacaggaa taactacttg gatcgggatt ttctccgatt ctgctttttc tagaaatttt
    15301 aaaaagacac ccctgacagc tggggataca atatttgtta gctgttgtat ttgcggtttt
    15361 tcaaaaatag ctgcagaatg atcttctaat atttctaaaa attccaacgt ttctttttta
    15421 ttttcctgaa gagtaatttt taatgaaaac agtagacttt tataatcatg tttgaattcc
    15481 tctagatgat ggtaatattc ttcatttatt tgatacagtt gaagcgattc ctgatgttgt
    15541 ctttctttta cttggaaact gatcaacata atggaaaaaa acaaagaaaa aaaactgatg
    15601 actatcgaca aaagagatag aagcagataa ttagatgagc ggaagtgaac acttcccaat
    15661 accaagtaaa tcacgcccca gcttaataaa atacaaatac ctccaaaata gtgttcagtt
    15721 ggataagtaa aaagcaagtt gagaaatctc gtctgtttgt ctatactttt tactaataga
    15781 tagaaaataa atgcgataag tagtgcaaaa aaacttgctg ttccaagatg tctctttcct
    15841 aaataatcca tcagatcaaa aaccagaaaa aagctgagtc cagtgatagc aaaaaaaaga
    15901 ttactgatca aacagctgat aaacaggttt ttcgtagtaa aataaaaaag aaacatcaat
    15961 aataagccaa ctaggtaagg aaagctcact ttagggatag gaagtaaaag tgtcaccacc
    16021 atcaaagcta tacaaccaaa gctgtttttt actagcagtt gtttcgaaaa gaataaatag
    16081 atacctaaat ctaaaatcaa agtaaaggtc aaataaagtg tcactgttgc gaccatctta
    16141 ttcctccatt gttttgtttc aaaatgtaca catatttttt taataggagc ctgcatatga
    16201 aaaagtattt tatcatagaa gataaccagt ttcacagcga atggatcatc agagaattgg
    16261 tcaatcatca tatcgagaat atcgtgcagc tcacttctat ctctcaaatc aatcagttga
    16321 tccagttggg cactattgat aatcacgatg ttttctttat cgatatcgac ttgaagcaat
    16381 cactcacagg tattgatgtc gccgaacaaa tacgtaaaaa aaatgcacat agtaatattg
    16441 ttttttcacc gcttatacga attttgcagt agagatcatc aatcggaaaa tccttccgat
    16501 tggatatatc agaaaagaca ggaatttatc tgaacaaatt cgtcaagtgc tagaacagat
    16561 caattggaaa gaacagcaga tcgtaaatgt cgataaaatc tttattgatc aaaaaggtgg
    16621 aagtctcgtc ctgtatcctc gggaaatttt atatattgaa agtatcaaag gtttgaaaaa
    16681 ccaagttatg gtcaaaacaa ctacagaaga taaattagtg aatacttctt taaaacaatt
    16741 gaagacagag ttagaaaagt atgcttactt tttactttta aaatcttact gtatcaactt
    16801 atcgcaattg cagaaaattg attctgctca ttatgtgtta gaatttttca acggtgactc
    16861 cttactcgtt ggtcgaaaga tctttgaaaa gacaaaggaa cgtttccatg acttccaaaa
    16921 aactgcttcg agttgatgaa taagagctga gacgtaagca tagcagattt cagggagtgg
    16981 gccttgaatc ggctccactc cctgaaatcg ttgatatgat tttacgcttc ctcttctgta
    17041 ggagcatctg ttaacgacca tgtgaatgtt gaaacgtagt tgtctacagc ataattggat
    17101 acaggaacaa ataattccac tgattgtctc ccttgttcca cattctctcc taatgaataa
    17161 atccatgtgc caatcccttc atttgctcct gcagttacta gatctactgc ctgatctgct
    17221 gggatttcga ttccttcagc gccagttcca gaaacggtag atggcattcc actgctatta
    17281 gttgcgctat ttcgaatcac ggcgtttttg aatttcacct ttgaaccagt tagtttattc
    17341 gtatcagatg ttgtaccaat aaattctgtt cgtttgactg ataatttcca accggcataa
    17401 tttccgcttt tatccgttac ttgtacgaaa ttctgtactt gtgtttgtgc tccagtactg
    17461 tccgttccgc tttgtaatgc agcatatact gtttttgctg tacctaactt gattgttcca
    17521 aaatcaagat ctggagcttg gtcaatcgtc agtaatccgg atgttccctg atttggttcg
    17581 tcaggtgtaa aaggagtatc aggatcattt ggatcaacag gaggaactgg aacaacgtct
    17641 tcaggatttg cagcatctgg tttggtcaca gaaatcgttc cttctgtagt ggctgagctc
    17701 ttattctcag caaaaacact ttgtgtacat aataatagac ttccagctag aacggtactt
    17761 aatgcaattg tttttttcat ttctttttcc ttctttcttt taaaataaat aatccagcaa
    17821 ataagccaat aagaatgact ccaacaagta tcagccatcg ccagtcaaat gtttctggct
    17881 gattttttaa atcaatatcc ttttcattca attggtccgc tgcatcatca gttatttcaa
    17941 aatcggtatg gagattccag cgtttttctt tccaagcacc cttcacaacc gcttgataat
    18001 gtcccggagc taattttttt ccttctaatg ggacaagtaa atcaaaattt gtattgggtg
    18061 ctacatctat tttttctttt tttattgaaa ggtaaggctc ttttgtttct tttttgtaga
    18121 tttcaaccga tgtagtcaag ccgaacatat aagaagcatt tggattttga agattcgcct
    18181 taatcacatt tcttccatcg ctttgatccg cataagcttt ttttaatttc aaatctgggg
    18241 aagactcttt ttcgctattt caaatcaaga ttgctttcac ttgagaaaac tgattgacga
    18301 caccaactgt acttttgtta gaaacgttct cttttttttc ttgaaacgtg atccctcctg
    18361 cgaccacacc agccagctct ttttctggcg ctttcagtga aagtgtctca gtgaccgcac
    18421 tttgaggagg aacagttatt tctcccactg tttttgctaa gtttttcata tcataactta
    18481 atgtttcatc aaatttactt tttgattctc catattcgat cacaccgttt gaattcgtcg
    18541 tagcggagtg gatcaatgtc tcaactgtta ctgatttatc tgttcggtta aagaccgtgt
    18601 aggggatttc ctgtgttttc ccaggctcca tccttacatt tatatatgtt ttgttcttat
    18661 caatctgatt agccggtaat tcaaaatcta ccgcaaaatt gaattcggag gcatggacag
    18721 gtgtctgcca aaggatgaat ccaaggagaa ggatcataag taaagagcag ttgtttcgat
    18781 gacttttatt ccatattcga ttcatcaatc atcctccttg atctttattc tgtcggcgca
    18841 tctgctaatg tccatgttat ctctgctgag taattaccag ctatctgatt ttctttggtt
    18901 aagacaagtt taaacgcccg accgttattt aaatattctg aataatctgc ttgcacattc
    18961 gttgaatttt gtcctctgac aatttccagt tcttcttcat tcactaattg atactcctct
    19021 cctttcttga aatataggac atcctgcata gaaaatcctg catcatttgt gaaaggctga
    19081 gataatttta tcgttaagat ccacggattt ttcgtttctc gctgatcata aatccataat
    19141 ttttcctgta tatccatttg gccaaatgct tcttgtgtca aaccagttgc tttgatctga
    19201 ccaaaatcga gtttggaagg ggcaagaaat gagagaccac cttctactac gatcttcgta
    19261 gcaggtgcaa aaggcggggc atcatgataa acaactggcg tatcacttgg attgtgattc
    19321 ccaatttcat cgttggtact tggcttgttc tgcacaccct tagcagtagc ttctcctgct
    19381 tggtcgttta atacaatttc taatacatct cctgcaaaaa gttgattttc tgggatagaa
    19441 agaacaaatt cccctgtatc atcaatagtt gtttctgaca aaacagcttg attttttttc
    19501 aatgaaacca aggctcctgc ttcacctgta cctgtgatcg tgcgagaagc tgcattgatc
    19561 aatgtggtac ttaaattagc aggttcagga ggaatcacat cgactgtcgt tttggcagag
    19621 tccagttctg tcacttcttg gccacctctt acttttttag cagcaacaac ttgcacttga
    19681 tcctccgttt ttaatctctc atcattgggt acttgaactt cgaacaagcc gccttgttcg
    19741 ccttctcccc atgggttata tcctgcccct tcattttcat ctgtttgtcc ttgcgtgaga
    19801 gcagtaattt ctacagatga tttcgtcgta tctgcgtaag taatacgtaa agtcacttct
    19861 acttcgcctg tccaagcatc tcttggcttt gcctcgacac cttcgggaac aacgacatgc
    19921 ccatagattt ttttatctgc attcgttggc actcttaagg attcaatcat cgggagctga
    19981 ttattggcac tgattcgatt ataatttttc attccggcac ctgtctgagc aaattcattt
    20041 gttaaacctg cactagggct tgatacgaaa ttgcctaagt cggtcccatt gatcgataag
    20101 tctgtcatat cagaaaaata atccgggtct ttttcaaagt tatcgaatgt tgcgcctgtt
    20161 tttcgccata aagcgatttc tgaatgggcg atctttaaag aagaatctcc atttccttgg
    20221 aatacccagc cgcctatgcc attggtctcg cttccaatcc gtttgttttg gaaatcaaaa
    20281 taggtaggag attctaagtc cacattcaac acatccccac tgatgatgcc ggtcttatct
    20341 tcccctgttt tcccgaccat gactaaataa gttttttcac ctgccgtaaa atttaaatca
    20401 atatctccag ttgtatctac agttgctccg gaatctgcgc gtatatcgat atttgatttt
    20461 tctccagtga ctgtaaaatc agctgaacct ggactttcta gaaaaccaga agcgtcataa
    20521 aattggatcg cttgatttcc ttgattgagt ctgttcacat aagatttgcc atcacctgta
    20581 tttcttaccc tcaatgagcc tccattggtt actgtcagct tttgcgtacc attctcaaaa
    20641 cgaactgcgc tggaacgccc actctgctta tcgatgtcga taactgatcc ggctccttct
    20701 acgatcaaag aaaggccttt tcctacaaaa cgcatcccat tgttcccggg ttgatcgtca
    20761 tctccagcca ttttgaccga gatttgtcca ccacttgata catggatttg actagaatct
    20821 ccttgcaagc gaaatccgcc cgtagttgct gtattgatgg tgattttccc accattcgat
    20881 acagtgactt tcgatcctga acctctcatt tggatgattc cgcgattgtt gtcattggta
    20941 atattgatct ttgtatttac cgaaaattcg cttcccgaac cactgacttc aaacatgaca
    21001 ttaccaggta gcgtatcgac tgcctctcct tcatcgctaa cgccatctgc ccgatcataa
    21061 tcaagagaga cctttgaacc gcttaagata tggattcctg gcgctgtttt tccactacca
    21121 gtctcatctc ctgaatctag aaaaccgtag aatttcccta gatacggtcc ataaagttga
    21181 ctaccattga tcacattcaa ttgatagctt ctacaaaaat aggctttacc cggagcggca
    21241 ttcagtcctt ccttcactgg ttggacatcc aacacaccgg aaacaccatc taatgtgaca
    21301 gttgcacgag gagcgtaaat cagtcctaat ttggacccat catacaaatc aagtgtcccg
    21361 gtaaatttta tttctcccgt aaaatgatat ctgcttcccc aaaaatcggt catataagca
    21421 atgataatgg catttccatc gatatttggt gtcaatccac ctggatgtgc agctgttcct
    21481 ttaaaattct ggaactcgat cgactgaggt atctcattga tataaatatg gtgtgtgccg
    21541 aaattgtgag cgaatccacc aaaatcaata acgatatctc gtttgatctc taattccctg
    21601 tctaaagtca aaggctgaat cagtcggatc ttagttactg attcgtctgc gagaagctgc
    21661 gccagttctt ctgttgtggc agcttcgctg tacccatccg ctgaaacagc tcttgccagt
    21721 gccgcttcac tgctatcaga cgtttgttca gctacttctg aggtctcact tgtcttattt
    21781 tcttcttgcc ttttattagt agaagcctca ctttgagatt ctgtagtttc agaagtaatc
    21841 gattcttctg aaaaagtagt cttgtcaaaa ctttgattct ctgattgcgt tgattcgttt
    21901 gttttctcta catcggcaaa aacggatgtc accatcaaga gagggaagac aaacaaaatc
    21961 gacgcaatca gcttttttac acattgattc attctccacc atcctctctg acgttttgac
    22021 aattttattt tatcgtttta atctatttcc aattgtactt tcatatttaa aatatgtatt
    22081 tttatggaaa aatttttcta ttaggtgtaa atttcttttt tgtacaaaaa aaaggctgtg
    22141 acaaaagtct tgtcacagtc ccttatcccg aacaagcggt gttctcccgc caactcctcg
    22201 gtcacaacct ccattttaac aataaacctg taaatgtgag ggcaatactt tgattgtcac
    22261 aggtaattta tccccttcat ctccatcaac attcgtcgtc aattcttcct catcagctaa
    22321 tgacacaact aattgctcac aagtaaggta ggctagattt ttcgtagatt catctactcc
    22381 ttttaatagg tctggcaatg ttttaagcgt atcgatcaaa gaagaatctt ttaaataaac
    22441 caaatggagc ttaccatcat ccacttttgc atttggaagt aatgtttcaa aaccgccgac
    22501 tgagttagtt agtcctatca gcaaggtact gctctcaatt tcttccttct ttccatcaac
    22561 ttctacgtga aacgagtagg attgtgtgga agctaactgt ttgattcctg acatgaaata
    22621 agctagtttt ccaaatttcg tcttcttctc cgcatctaca tcattgattg attcagggat
    22681 agagccaatt gcgacgacat tcataaaata gtcgtcattt atttttccaa tatccaatgg
    22741 cttgactgat tcaatcgaaa aatgattgat cgcttcctca ggttctagcg gtatccctaa
    22801 tgctcgagcc aagtcattca ccgtacccaa tgggaaaaat ccgaaatttg gccgatgttc
    22861 ttgttcagcg attccactga ttccttcgtt gactgttcca tctccgccca tgacaaacac
    22921 actatgatac ccttccgtag ccgcttcgcg agtaaagttt tttgcatccc ctgctttttt
    22981 tgtatgcaag acgaccactt catcaaacac cgattccaat tttgctatcg ctaattgctc
    23041 aaattccttt gcttgttcac cgcctgaact ggggtttacc actaataaaa cttttttcat
    23101 cactttcttt cccttctgat tgatttcttt aatataacga tagcataaag aaaaaaatta
    23161 ggctgtgatg aagtactgtt catgaaacat tcatttattt tttgacaaga taaaacatcc
    23221 atactatgaa actgaggaaa attaaaacaa tgatatagga aataaaagaa aatgataggc
    23281 attgtatacc taataaagta aaaatcttag gtggcagata aaaacacaaa aatagcaaga
    23341 gaatgtaata gcgtttcttc cactttttgt tatcagacct tatagcgatc agaggaatac
    23401 caaatgcata actgaaaaac tcttgatgta ctgttaatat gtcacggcct tgaataataa
    23461 atgcgacaat ccccgctata attagtgtgc ctaacaccaa aacggaaaat tgatgaatat
    23521 attttatata caaatgatcc attgtacttc ctgccttttt atttgtgaga aaaacaaatc
    23581 cgtatacgtg aataacttct agaaataaaa gaaatttgca aaaccactcc atttatctac
    23641 ttctacgtgc attttttgat attgttttct ttatttaaga ttatacagaa aaatatctag
    23701 ggtcgctgga ttctaagtaa aaaaaatttt taaaataaaa agaactaaat caagtagtaa
    23761 ttaaactact gttgatttag ttctaataaa aagatttaga tgaacagttt tattgattta
    23821 aatagttcat attttttatt tataccgctt atttactact tgctgtacta gaatctgaag
    23881 tgaatgtact caaaacattt tcaaagtctt tgtctttgat ttttacatta gcgtctttca
    23941 atacttcgcc aattacttta ttctggaagg tactgtcact taattttgtg tctgttgcaa
    24001 tttctttcaa ttcttttttg tatttatcca tgtcgttgcc tttgttttgg tttttcacca
    24061 ttttaaccac ataatattcc gtagtataag ttgaagcatt ggtagaagtg atcacatcag
    24121 aaacttgacc atctttcaac ttaaaggctg cttctttgac ttctgccgga accgttgtag
    24181 tagtggaatc aaatttcact tttccgccat cttcttttgt cgtagtatca gttgacttgt
    24241 ctttggctaa tttagagaaa tcgtctccct tatcagcagc tttcttcaca tcttttgcat
    24301 catcttcact tgctacttgg ataatttgag cttccacttc tggatggaaa gattcccaag
    24361 ccgtttttaa atcttcatcg gtcaatttga tatgtttctt tagaccttct tgaaaggcta
    24421 gactttgttt gatactgtct ttgaatgttt gttctgtgta acctgcagat ttcaattgtg
    24481 aatcaaatga atcacccaat tgtttctttg tttgatcata ttgagaatcg atttctttgg
    24541 ttgatacgtc atctccatat ttatcttcaa atacttgata aacgatcaac tgttgcacag
    24601 cggaatctgc tccttgcatt tttttcaagc tatccattac ttcttgttcg ctgattgtac
    24661 cgcctttata actaacggct gttttttcac cggcattcgt acagcctgac aataagccag
    24721 tgcctagtac gatggcaagt gctaaaaatc tttttttcat ttatcaattc tcctactttt
    24781 cgttttttgc gaattgctaa aaacaaaggt gttcaagatg aaaatcatca agaacacctt
    24841 tactaatctg ctcgatacgt cctgaataga tataaggcta cacaatccag aaagttcgct
    24901 cagcagaata aatgtcaagt agcacatcac accttaatat actacttctt ttgttaccgt
    24961 ttttcaagtc tatcttgcgt tccgtattta acgtaactaa ttcttagaac aaatgttatc
    25021 gattttgttt ttcttcttcc gctgctggga tattcaaatc agacactttt atgttcgatt
    25081 ttccagacat ttttttgttg tttttattat gatatatttt tatacaacta aaaatgaata
    25141 tattaaaact accaaaaata acttttaacg ctgtttcatt atatgttttt tctattttat
    25201 ataaactttt ggatacttaa taccaaccgg ttggattgta ccaacatgag aatgtgtcag
    25261 ttctatttcc tgtcgcactt gcataggccc atactccttt tgttgcattt gctgatcgaa
    25321 tagagtttcc aactttcgct tttgcaccgt gacgattata gacacgacta tagtggttag
    25381 aatgacctgt ttttctcgta caagttgtat cactataata aacccatctt ccagagctat
    25441 gattaattgc tgttctagaa atttcccggt ctactggtaa atttgactta ataatattac
    25501 ctttagcatc aaaatgaatc tctcctggct ttaaagattt gtaatagatt tcaccgtgaa
    25561 ctgaatttgc ttccacttca gatgacatag cgtttcctcc aacaaaacct atccctagtg
    25621 cacaaataat aactgctttt ttcataaaac gacatccttt cctttttgat ttttaaagca
    25681 tgatcttctt gtttcctatt ttggtcagat atggatcatg cgtgactacg ataactaacg
    25741 taccttggga agcttgttgc gataagattt caactacttt atccttattt cttttatcca
    25801 atgacccagt cggttcatcg gctaaaatga ttttgctatt cttcaataag actctcgcac
    25861 aggcgattct ttgcttctca ccaccagata atgtgtagat aggcgtactt agcttcttgt
    25921 ttatatttac ttttttcaat gcctcatact ttgctgttcg cttatctgaa acatttaaat
    25981 tcgaaaactt caatccaata tctaaattaa aatcgattgt ttcatattca atcaacccaa
    26041 aatcttgaaa aagaaaagat atggtatttc tttttatcaa gcgcttctct ctcccagtta
    26101 attgtttcgt ctcttgatcg aacaaataga attgtcctga actgaactga tcattcaatc
    26161 ctaatatatt caataacgta cttttacctg ctccactttc tccaacaaca gtcacaagat
    26221 ccccagcatc cgcagtgaaa cgatccaaat gaagaatcgt tttgtttcga atgatttttt
    26281 tcgcaccttt tatcactatc aacggctcca attgttaacc tccattcatc agcaataact
    26341 gacttttctt ttctgtagac aaaataaaaa caactgaaag aataaaatca atgaagctaa
    26401 agaaaaaaat attaaaaaga tatttaggct ctctttctat aagaaaaagg aagattacac
    26461 ttgtcgttcc ccatttgatg aggttcatca tgaagaaatc tttatacgtt tcaaagagcg
    26521 aataaccact tgaacgaaag atagcgattt tccgacaatt gtagacaaaa tatatgcata
    26581 acatgacata gctcaacaag aagaaagaaa agaaagtgag taaaaatatc tgtatcaagt
    26641 aatgaaatga tctgcttaac actttgacag ctatctcttc agtctgagta tacggaaatg
    26701 ataactgcaa gttatcacgt aactgacttt cttcaatcag ctcaaataac ctttctttcg
    26761 acaaagagcc tatcttgatc tttaacggtg gatagatttc tccgctgaga atatttcttt
    26821 ccaaatacat actgtttttc aatgtcagta tttcaacaac cggataatgt tcgatccagg
    26881 ggatagaagg aataaatgta taaatgggtt gattgttttt tataaataga aatttcggtt
    26941 ctttttcact gatttcttga taatattctg ttatttgcgc caggtcattt tttaaatctg
    27001 tatgtctata tcgttcaggg atcaatataa cagggtgcgt ttctttttcg gttatttgga
    27061 tcaaccttcc agagacatca atcaaaggaa atttcttaag ataattcaga tttattttta
    27121 tccctctgcc aaactgttca ttttcttcat tcaagtaatc tgatacattg actaatatac
    27181 ttccgttttt attcaaagtt tcatataatt cctgatctgc tgctgatgca aaacgattgg
    27241 aataaataaa atccacgtgg tttttcccga tggttactgg ataaaataca tgatagtcct
    27301 caccagcgat ttcttttggt ggttcatggg aaacagctaa ctgtgaagaa gaaaaaacga
    27361 tagttgctaa ttcgacattg gcaacggtca aaagaagaga aaacaacaat ccaaatgtaa
    27421 aaagaatgta ttggatatag ttgaacgctt ctcttaacga agcgattttt tcaactaata
    27481 aaagagaaat caaagagata ccatatattc cgctgaacaa gaaaacgtac atctggaaca
    27541 aataaagaaa ttgctcctta taatacataa aaataaaacc accaatgatt cctgcaatta
    27601 tgaatgtaat aatgattttc tttttcccca ataaacacca caatacttga tgtaatgaaa
    27661 agtcattatt caacaataaa ctgatttctt tattcatcaa taagaaaaag gatgataaca
    27721 aaaggaataa aagaatcaat ccaatagtta aatacactcg ataattatat acgtctgtat
    27781 ttgaaataac cgaatacgaa tagctatttt caaagtcttc aaaaatatta gtggcataat
    27841 cagtgtgaaa tgcttgattg aatcgatcag tgaatagtga aatgacccgt tcataggctt
    27901 ctttttctct cgtcatcaag aaaaattcac cttcaaaatc ttttattttg atcgattctt
    27961 caatggacgt tacgcgaaag gaggtattag aatagggtac cttctcgtga gaagatttga
    28021 catccccttg ataatacaat gtcatttcaa gctttggctg tatcttgaag ttccaagatg
    28081 tatctacttc attggattca tttactgttt ttttcatata agaaagctca gtttctgatg
    28141 agacattttc aaataccatg attagctgct catattcttc ttgcccaata aaatgttgtg
    28201 gcaatcgaat cggagtataa gtatctgttt gatggaaaaa ttcatttgtc cgatagagta
    28261 cctgtttatt tatactaacc aaggcaaaac cactcatgat accggtcact atgattgtga
    28321 gtaaaactgt gaactttttt ttgattatca ccactctccc ttacttaaat gattctttga
    28381 taaaatcata ccgtttttca tcaaaaaaac gtatctatca agtgtatagg tcatgtagaa
    28441 taaatctata tactgtagaa tcgaaaggag aagaagatga acaccggaga aaaaatacga
    28501 acttaccgca gaaattgtaa gcttacgcaa aaagaattag gcgaaaaaat tggtgtttcc
    28561 gataaaacga tttctagttg ggaaaattct cgtacaatgc cagaccttga aatgctgtca
    28621 cttttacatc atactttagg tctgccgatt gattttcctt ctattgaatc agctaccgaa
    28681 tcaatagaac caacattgcc agatgaacct cttgttaaca agaaatctta ttcatcaaaa
    28741 caacccttct cactcaaaaa gacactatct ttctttctgc tcgtcttttg tttggtctat
    28801 ctttttctta tcaattatca atacaaaaat gaaacgatcg atcgattcaa tccttttttg
    28861 aaaccacaaa tcggctatac cacactccct gtaggatacg tggacaattc catcgaacgt
    28921 tgggtatgta atgatatctt tggccatggt acatggctgt cttttcacgc tggagaggta
    28981 gaaaaagaga aaaaccatgc tgtaatcaaa cacaaaggaa aacacgtcat gaacgttcgc
    29041 ctgctcactt gggaagccat tccctcagat taccaagaaa tcctcagcca agactatgac
    29101 gacgccttaa aaagaagccc tcaggagcca gataggcatt aagtaggaga tgaacgtaga
    29161 gatagaaaaa atcaatgcac atgatcttat ccaaaaaaca ggggtacaaa ttaattgaaa
    29221 aagccttgag atgaaagttc ctaaataaaa aagttcagaa tgataggtat ttctggctta
    29281 tagtataata tttacgccac agcctcccaa tgattctttt tggaatcata atcaaggatg
    29341 gacattcaca aaaatatttt gctcattcga tccgaataga ttaacgacca atctagtaag
    29401 taatatagat tggtacgtag tcaagctgca cataatttag atcaatttta gttctttttt
    29461 atatttctat gatctgctca tttactttta tatgatggct tacagtaaca taaataaata
    29521 tgataatcgc tggtagttta tccaagagta ctcaataaaa tacaaaatgg attgttcaag
    29581 cgcctatcat aatgataaaa tgaaacaaat aaacgataca aggaggaaga gactttgagt
    29641 aatggcagaa caattttttc attgtaaaaa ttgttcagtt gttaatctat gggatttcgg
    29701 tagcatgggc atttttttat agaggaatca caccaacctg gccgttttat ctcttgttag
    29761 tgggaatatt agtaggaaat tttgcccctc ttcactatcg tacgaacttt tttccaactg
    29821 attctggcct ttttcttaaa acaaagacgc gaaaattaga aaacattatt gaaatgacaa
    29881 ttacctgtat aatcattttc ttagttatat tcctaaacgt cttctaataa gaatctctta
    29941 aaattagctt gttacagtta gttacctagt ttatataaca cagttatctt atcttcctta
    30001 tcaacatctt ttgccaactc tttgtcttaa attcgaaaca tataagcaga gctacgaaca
    30061 ccaagactgt ttcggctctg ttttttgcat caaattcact cttatatagc tgataatctc
    30121 ggctctttaa aagaaattat tttcgcttca ttttattata aaaaggctag gaaccttgat
    30181 gttatcaggt tcctagcctt tatttaagta ttaccccaca gacccttcca tatagatttt
    30241 taaattacac actagtttaa actaaaacaa acaaggaaaa acttaccatt taatcaaaaa
    30301 attagctcat atgaatatat tagaatgtaa attagactgg tcaaaatctg gtcaaaaaag
    30361 aggacgaata caggtagtcc tcttttttgt tattttgata ttctaaatca ttttttattg
    30421 gattattccc ttgttcgata tagacccaaa ttgatctatc aataattatc aaactgagtc
    30481 atatagagac aaaaatttca aaaataacct gttaaatggt catcgaatat catttaaaat
    30541 caatacgacg gagtcagaca cggagtcaaa acaagcccgc tgagagtaaa aaatatacag
    30601 tcccataggt aaagtgccac aggactgtat atttttttat ttgagattac ctgaaatgat
    30661 tccatttaat ttttatctca ttttttccta ctagttagta tcaaatactc tcctattttc
    30721 tcaataaact attttgggtt acatggtatc tgaaacagct aaaaccttgt cattatagca
    30781 aaaaatcact ttatctccgt ttcatttcag atacatttca cttaagcgga ttccaaaaaa
    30841 acaaaagaaa aaggaacccc tagttagcct atgcttttta ttggttcctc tcaaaacacg
    30901 gtcatataac aacggggtgg ggtaaatgaa tgaaaaaggt ggaggaaaca ttattcaata
    30961 gctgtcttaa atacttcgtt ataaaatgtg aacaaataaa agacatctat ccctattttc
    31021 tacacttcca cttgactacc atctttgaat cgtacaattt tcttaactct tctcgtttac
    31081 ctcaattcat tctcaagtgg aatcaactca tcatctaaca ttttacgcca tgctcgcttc
    31141 atttcttgtg ggtatttaaa tggcttcgct tctggatgtt tggctttata tccttcgtaa
    31201 tccttactgt tgataattgc tttcatatta ggaattggat cttcacctgg tagatattgc
    31261 agattagaag catgtaattc tttctcagaa acaaaccact cattggaaaa gacatgaatc
    31321 aaatcatcac tcgcttgtgt aaaatagcga cgtttaatga caaagatatc ttcattacta
    31381 tctaaagtcc ccacatcaaa tgcatttaaa atttgtcggt aaacttcttt gactccttct
    31441 gttttattca atttagccaa agctttttca atttcttctc gtgcatctga actattcgca
    31501 gcataagttc ctagtaattg atcaatataa gttgaatcaa tatcatacaa cttagtggta
    31561 ttttcactat aaaattcgat cgttgaaaaa tcttctcctt ctttttctgg ttcttcctct
    31621 attaatgaac ccttcacggt ctcgtacacg ccaatttgtt cttccaaaag ttgaatttga
    31681 ttgttcaaag ttggagattt caccatgtca tcattgtaat cgtcataagt cactaacgat
    31741 tcatacgcac gattcaattc ttgaaatgct ttgacgtact cgatacggat ttcaagtggc
    31801 gtatgttcat caatggattc catggatgaa acctctttta acttaccata tgcttgtttg
    31861 aatctttttt tggattcctg ataacttgga taaatcaaac ctgattcttc ttttgctttt
    31921 gaatacagtt ttgtcgcatc ctcgacattc ttctccattg tcgctggctt acgaaacgtc
    31981 acaatcaatc cttttgtctt atcaggatac gtacgattcg tacgtgaaaa agcttggatc
    32041 aatcctgcat actctagatt acgatccaca aatagggttt gaatcgtagg ggcgtcaaaa
    32101 cctgttaata gacgatccac tacaatcgct aaatcgattt gtttcccaaa ttgtttgaat
    32161 tccgctcttt ttcgtgccaa acgattattg atgtctccgt tataacgctc aatgtctgct
    32221 aatgaccagg aagtatcata atactgatta tattcctcaa taattttttg catttcttct
    32281 tgttgggcac ttgaatctcg gctattttct tctaatgaat aggtaatagc gattcgtgga
    32341 aaatcaggat cctccatcgt tcgtccttca cgaatgagct gattgggaaa ctcttttgtt
    32401 aaccaatcag gctctttcgt catctctttg atggcccgat aatacttttt ggccatgtca
    32461 atggaactcg tggttaagat tgcagatttc gttggcctgc catttcgaaa atcaaatttt
    32521 gtataggcat tatctggacg gaacatttta tgaatgactt tttggatatg ttcatctgct
    32581 tcaaaaacag ctgggtccaa ataactttct tttttgacag gttccatttg atcaatcata
    32641 cgattgatct gttctgatga atactctgca tatgtttcta cttcttttaa tttccgatag
    32701 attttattct ctagtgaagt cggctccacg gtgttttcat gctctacttg aaatcctaaa
    32761 accgagccat cttctaatgc attcttgatg gtataggtat gcaagacctc tccatactga
    32821 tcatgggtcg tacgtgccaa ttgccctttg gcttgtttcc gattctcagg gaaaatcggt
    32881 gtacctgtaa agccaaacca agtggatttc ggaaacattt ttttgatttc ttccatattt
    32941 tcagcactta acgctcgatg acactcatct actacaaaca cgatatgttg ccccgttaat
    33001 ttttggaagc gattggttcc ttttttctct tcttgtttct ttgcatattt caacgctgca
    33061 tctagctttt gacgggttgt aatgatcaca acatttgcat tggcatctgc caacagggtt
    33121 tcacttaatt ctttggcact tcctgttccc acgatcaagc tattcgcttt ggcattacca
    33181 gaagaaatcc cagtattaaa ctccgaagca aatttcgtaa actctgtggt ggtttgatta
    33241 tctaaatctt tgcggtccac taacatgatc gtccgatcga tccccgtttt tcgtgctaaa
    33301 agcttagtag atacaaaact agtcaacgtt tttcctgaac cagtcgcatg ccacacatac
    33361 cctgattgat gtttatttgc agcaataaac aaggcttgaa tcgcatgtac ttgataagga
    33421 tgtaacacca tgaggatttt attgtcttga tcttcactga caatcgtata atccgcaatc
    33481 aagcgatgag catccgggat atttagcact tgtttgcaaa actcatataa gttttctacc
    33541 tttttattat ctttcgttcg ccaactaaat aaaaacttag gatgcaaatc ctttggtaag
    33601 gcatttgcaa aataccgagt ggtttgttca ttggaaacca caaacaactg taaggtcgaa
    33661 aagatattgt tccgaaacat accttcttct gcatattttt tgatttggtt aaacgcctga
    33721 taaaaaccat cttttgcggt tacttgtttc agctcgattt ggacaatcgg taatccattg
    33781 atcagtaagg tgacgtcaaa acgtcgatcc cgtgcttcta tgttacttcc acgttttgca
    33841 atttgatgga ccacttcata agtagagatt cctccaccaa tgtcttgatt ggaataaaga
    33901 atgagcgaga cagaacccaa ttgcggatct tcccgttcaa tcgtgatccg agccattcca
    33961 ttttcacctt ttaaccattt cgcagcatca aagggggttt tcgttcgtag caataattcc
    34021 gttttgatcg tttcaaattc tttatcggtc aaaggaaaat cattcagttc tgcttgatta
    34081 tttgagatga ttttttgacg taaatttacc caaaggtctt cttctgattt tagatcggga
    34141 cgatacgtcc actgattatg tccttccccc agtacacgaa tcaactgttc ttcgacttcc
    34201 gcttcctcac ggtgactaat tttcattctt cttccctcct agacaaacat cttttgtaag
    34261 aagccttttt tcgtttcctt cagcaaatct aacttacgtt gatgaagagc gatcatgtcg
    34321 tcgagttgtt taaagaagtt accgattttt tgttgttctt catatttttt tggaatggtc
    34381 acttttttct ctaataaatc tgacttagtc attcctttaa tagatgttcc ttgtatattc
    34441 ttaagctctt tttgaagcat agaatataaa gagaagattc caaaatatga atctatttgt
    34501 aactctgaaa gtgataaaaa atcttggcta gtagcatact caaaaggcat taatgctaat
    34561 ttaccaaccc ccactcgtgt aactatcgca attgagttag ggggaataat ttttgctgca
    34621 gattttttaa cagcttcatt cgtaattttc tttttaggag aaacattaaa taatctgtta
    34681 atttctaaat cgctactttg aatccatgga atgttaccgt tccaaaattc ttttgtatta
    34741 gttttaggag taccaccacc ataagtaact tttgaaattt caaaaacctt acgctcttcc
    34801 caatcttccg taaaaccagg aaaacggatt tctggcactt ttgctccgtt ttcagggaac
    34861 attttttgta agaatccttt tttcgtttcc ttcagcaaat ctaacttacg ctgatgaaga
    34921 gcgatcgtgt cgtcaagttg tttgaagaag gtgccgattt tttgttgttc ttcctcacta
    34981 ggaaacattg ctactatatt tttaaacttt tttagagaca gaacatgttg acctgttccc
    35041 tctgcttgaa tagataattg tcgttggatt ccattgttaa tacgtcttaa gaagaagtcg
    35101 gaatcagcag attttaaagt aaatacagga taaaaatagc ttataatccc tctatcaata
    35161 atgtcattac ggttaaacac aaacacatca ttatcacttc gactacggaa cgtgaaatat
    35221 cctctaggta gtacaaagta accaatatta ttttctgtag ttacttgccg atttgcaaaa
    35281 tagtcttctt gcaaaactat ccctttttgt tgagatgaag ttagcatagg aaattgattt
    35341 tgtacacttg tcttttcaac ataatctaca acgaagtccc ccaacttcct ctgttcccaa
    35401 tcatctgtaa atcctgggaa acggatttct ggttgtgtat cattgctcat gtttgaacac
    35461 ctccagcgca ccttgtaacc acgcttcatt ttcaggtgtt gtttgcaggg aagagatagc
    35521 ttctaaaagt tttttttcta gcacttgctg ttcttgtcgt atcttcttga tgtcatttcc
    35581 gacatgaacc atatcaattg gttcttcttc ttcgaaagta tctacatagc gtgggatatt
    35641 taagttatag tcattttctt tgatttcatc aaaggttgcc aaatgagcgt atttctcgac
    35701 atcttttctt tcagcgtagg tttcgaggat tttttgaatg ttttcttccg atagtttatt
    35761 ttgatttttc cctttaacaa attcacgact agcatcaatg aataatacat ctcgagtttg
    35821 acgattcttc ttcaacacaa tcaccgtcgt tggaatcgat gtcccaaaga acaagttcgc
    35881 aggcataccg atcacagcat aaatactgcc atcttctagt aatttttgac ggatgacccc
    35941 ttctgcagcg ccacggaata aaactccgtg tggtaagacg atggccatgg tacctgtttc
    36001 cttcaaatga tagaacccat gaagaagaaa cgcaaaatct gctttggatt ttggcgctaa
    36061 tttcccataa cgattaaacc gagaatcatc taagaaagtc gtatctgctg accaattagc
    36121 cgaataaggt gggttcatta ctactgcatc aaatgtgtaa ggttcatctg ttggccagtc
    36181 cttattcaac gtatccccat tgcggagatt catttcttcg gcgtcgacac catgtaaaat
    36241 caagttcatt tttgctagat tgtatgtcgt tgtatttagt tcttgcccat gatatttaac
    36301 gttatctggg tgtgttaagt agttacgtac gtttaacatc aatgaacctg aacccatcgt
    36361 tggatcaaaa acactaaaga atcgtcgttc cttttgatcg agcgttacga tttgcgccat
    36421 catatcagaa accatatgag gtgtgtaaaa ttctccggct ttcttccctg cttctgaagc
    36481 aaattgactg atcagaaact catacgcatc cccgatcacg tccccatcgt gttctaaaac
    36541 atctacatca ttcaatttct tgatgacttc ggtaatcgtc acatttcgtt gttgctcatc
    36601 tgttcctagt ttttttgatt ggagatccac atcatcaaat aacccattaa attgattgta
    36661 ggtagatgcc aattgcacaa aggctttgtt tagatcgttc aattggaacg ttgcttgttt
    36721 tgcttgatcc gctaagacgt tgaaaagata ctctggtgca attgcatatc ccaaaatatc
    36781 aacgattgtg gcaatcagat cttcttttga ttcttcatct gaaagtaatt cacgatacaa
    36841 cattgtttgt ttgcttactg tgtcatattc ctctaaggat tcatctgcta aaagaaccac
    36901 ttgttccaac aatcgatcgg ataaatattt atagaaaatc agtcccaata aataattttt
    36961 atattctgac gcatccattt tactacggag attatctgcc gcactaaata atttttgatt
    37021 taattctgca cccaaaaaga tacactccta tctcttattt aaagttgcta tataccctct
    37081 tattttagca tacccccttt tataaaaagc aaaaaaaaaa gaaagaaact tattcttgct
    37141 aataaactac gcgaaaggag tctaacgttg tttgatataa cttagactga tcgttaactt
    37201 attttttata tttctacata gttatcttta gaaccatttc agatacatat gaaacaatta
    37261 catatgattt tttcataaaa aaacagtact gtctagtagt ataaaatata caaatttctt
    37321 aaaaattttt tcactaaaat cactacctga aacagtctaa aacacttata tatcaacgtt
    37381 taatgaaact atctccgttt catttcagat acaaataaat atagtcgggc tccaaatccg
    37441 aaaaaaaata cccccagtag cttatcctaa gctactggag atgtataagt acggtcatat
    37501 aacaacgggg tagggtaaat aaatgaaaaa gatggaagaa tcattattca ataacttttt
    37561 taaatacttc gttgtaatca gttacctgtc cgttcttaaa taccacagaa taatcttttt
    37621 tcccattttc aagtaaccga agtggaatgt ctttaccaac ttgctctgtc actgcattac
    37681 tgatacctgt tagattagct aataaatctg ataaagattg agcatctgta gaaatctctg
    37741 tatcagtatt tatcgtgatt cctttatctt catttacaag cttccaagta ggtggtatag
    37801 ctgtgtatgt ttcgatgaat gaatcaattc tttcatctat cgtcttccca gtatctactt
    37861 ctgtctcatt actcttagat gaatcgattc tttcatccat cgtctgctcc gtaactgctt
    37921 ctgtctcatt attctcaccg gaaactctct tcacacccaa acttatatat tgtccatctt
    37981 tagtgtgcat agttaaaatt cccacgcttt tactaataaa ttcactataa ccagttcttt
    38041 caatagagac ttcaatgtat ttcacactat catactcaaa agaaaaatag acatgtaaaa
    38101 gcattttgtt gtttacatct acataataat cctttgactc tacttgagac cttcctatac
    38161 ggaatacagc tttactgtct gatatctcta taccagcatc cttattatta atctgccaat
    38221 ctccaagtag ctcttctggc aagctagtag tttcttgtga acactttacc acttcactgg
    38281 tagaggtcga aacattcacc tccgcactgt tagaagaatt ttccaaggaa tcaggagcct
    38341 tagcacaacc aacaagtctt tatatattgt gcagccgata ttttcttcat tcagtacacc
    38401 cttgaaatat gcagtgttag ataaagcagt tcgtcctgat ttgctgaggc acccatctct
    38461 tcttgaacgt actccttgat tttcaaggcc gcccgatact ctttcggaaa acgctgcttc
    38521 actgaagcca gcaaaaaatc atccttctcc ggcaactctt ccttcttaat cattcgggca
    38581 ataaaaaact gtaaatgcgt cacaaatctt aagtaaagca tcgaatcttt atcaaccttt
    38641 ttttcgggaa acagatatcc tagcatagcc aagatatcct ttagtgctct taccgagagc
    38701 atggtttctt ccatatttgc ttgttcactt tgggcattta caatgtgaaa cgcaatgttt
    38761 ccagcctcct cttctggcaa ctgaacattc atttttttgt tgatcaattg gatagcatag
    38821 cagccgatct caaattcttt tgggtaatac ttttgaattt caatcagcag gcgattttga
    38881 aaaacaatgc ctttttgact gcgttcaatg gcatgcttca agtgatcaac caaggtaaaa
    38941 aatagacttt tattcagctt aaagtcaaac ttggcgttgg cataatcgat aattcgcttg
    39001 acgcaaataa ttagatcatc cggagcagcc tcaaacagtt taatgtaatc cttttttaga
    39061 gcatcagaaa gtgaatctaa tttaaatact ttttggattt tgtcagccgg aaccaaatcg
    39121 cctttcttta gttgaaatcc aatccctttc cccatcaaaa ttacttcttt gccttgctta
    39181 tctaagcaaa gaattaaact gttgtttaac gttttaatta ctctcattag attcaccgtt
    39241 ctctctaagc taagccattt cttgcgatta ctttttgata ccaaaagaag ctttcttttt
    39301 ttattcgttt taattcttta agattctcat cggttcggtc aacatatata aaaccataac
    39361 gcttattgaa gccttgatgg gtactaacaa catcaatcgc tgaccaaggt aaataaccaa
    39421 aaacatctac accatctgtt aaagctgctt gaatagccag aatatgtttg gctagataat
    39481 caattcgatc ctggtcatta attcgtctat cttcctcaac tatgtcgggt gccccatagc
    39541 cattttcagt aattaaaata ggtaaatcat agcgatcgta caatttcctc aatgttaatc
    39601 gtagtccagt agggtcaact acccagccat atggcgtttt atctacataa ggattttcag
    39661 ctgcccggta gacaccttct tctcccaaca tgatctgttg atcccccgct ctagcggaaa
    39721 catctgatcc gtcatttttg ctggcagcga tggtggcagt tgaataataa ttaatcgcaa
    39781 taaaatcggg tttaccggag gataaaattg cctgatcctc tgcctcaatt ttaggatata
    39841 atccacgatc attcaaatag ttttcaaaca aataattgta ttttccccga actgctacat
    39901 ctaagaaaga ccagcctctc aatgtttccc aattatgggc tgcaattgca tcttctggac
    39961 tggaggtagc ttgatacata gccgttaaat tgattgctgg accgattttt cctttaagat
    40021 tcaactgatg atacaagtga attactctag cttgagctaa taaaacatgg tgattaattt
    40081 gaaagagttc tttttttgaa ggcagctctc cacttggtgg caaaccaatc gctccaggat
    40141 gaaggatcat cgtattttgc tcattaattg tcagccaata gttcaccttg tcaccatact
    40201 cagtaagcaa tacctgtaca tagtccacaa aataatcgat agttgctcga tttaaccaac
    40261 cgccctgctc ctctagaaaa taaggtaaat cgaaatggta catggtgaca atcggttcga
    40321 ttccctgatt caggcattca tcaattactt gatgataaaa agaaattccc ttttgattgg
    40381 ttttattatt ttcaatatcc ataattcgcg tccaagcgat ggaaaagcga aaagctttta
    40441 agcctaattc tttaaacagc tgtatatctt ctttatagtg atggtaaaaa tccgaggcaa
    40501 cttcaaaatt agctacccct tctgtatgct gatacttgtc aataatagac ggttgtttcc
    40561 catcctcctg ataggcaccc tctgcctggt aggcactaat ggaggcaccc cataaaaact
    40621 tctctggaaa tgctgaaagc tgtttgtatt gcacaaaaat cctctcctta actaatttgc
    40681 atcaatggtt cgtgttcctt taagcttccg cttgcttcac taattcctgc gtaattttca
    40741 ctgttggtca ctaccacaat cactgtatca tcgtagccag cgtttctaat ggcagcgaaa
    40801 tcggcgttga ttaataattg tcctgctgac acctgatctc ctgcttttac aaatacttca
    40861 aagggttccc cttttaaatt cacggtgtcg atccccacat ggatcaataa ttcaatacct
    40921 tctttagaac gtagaccaat cgcatgttta gattctaaaa tagtaatgac ttctccctca
    40981 acaggtgaat aaatgctgtt ttcttctgga tgaatggcta cgccctttcc caatagtccc
    41041 ttagaaaaga cagcatcgtt cacttcagaa ataggacaca cggttccttt taccggattt
    41101 tcaacaatga ctgcttcggc tattgccgtt tgctctgatt catcagaagt atcgtcaaat
    41161 ccaattagat aagcaatgat aaaggttgca acaaaggcga ttacacacgt aataattgcg
    41221 tgaacaatat tcattggatt ttctccaata aaggctggta aggctgctaa gccaggggaa
    41281 acaaaggcat atctgactaa accgctcaag ccagcataca aaccagcaat tgcaccacca
    41341 atcatggaag caactaatgg ttttttcagc ttcaaggtta ctccatagag tgaaggctca
    41401 gtaattccca ttaaagcagt gatcccagtg gacacagcta gctgcttcaa ctctttgttt
    41461 ttagttttcg cggctacagc aaaggtagca cctgattgag caatattaga agccagcatt
    41521 cctggcccat tgatcatttc agaacccgta ctgctcaatt gcatagtagc gattggcgtc
    41581 attgcccaag ctgtacccgt aaccaccaaa aatggctgca aaccacccat caaaaatgga
    41641 attaaccaag ctgctttgcc gttaatccat tcagccccca ttgctataag atcgttcagc
    41701 aacgttccaa acggtccaat gacaaccaac gctaaaggcc ccataattaa aaataccagc
    41761 atcggcttag taaagaattt aataaacgaa ggcgaaattc gatccgcaaa gggctctaca
    41821 taggacatga tccaaacagt aaagataatc ggtaaaactg atcccgcata atctgcagct
    41881 aataccggaa ttcctagaaa agaaacggca tcgccagtag aaaaaagctg tgaaatattt
    41941 ggatgaagca aaatgccagc aacagtaatc gccaaaattg ggttgacatc aaactttatg
    42001 gctgccccat aggctaatag tactggcata aaataaaagg ctgcatcagc aatagtattc
    42061 aaaatgatgt aagtttgact actgtctgat agtacaccag tcaaagtcaa gacggccaaa
    42121 atggctttta ccatcccggc acctgttata gctggaatca tcggtgtaaa ggtggtagaa
    42181 atgacactaa tcaatcttga aataatactt tcttgattgt ctgaagaggg gcccttttta
    42241 attgaagttc ccacttcctt ttgaatttcc ttgaaggctg tttggacttc attgccaatc
    42301 accacttggt attgtccacc gctatccacc acactaatta caccaagtgt tttttctaag
    42361 gctttcgtat cggctttcct tttatctttg agttcaaaac gcagtctggt ggcacaatgg
    42421 gttaagctga ccacattttc ttttccccca acctgttgca atacatttct tcctacttct
    42481 ctgtaatcca ttattctctc tccttaaagc agtaaaaaag accaagtcaa accacgcccc
    42541 aatcaacatt gaagtgttag tttgtcttgg tcttgcctgc ataatcagta acatgccaat
    42601 aacgttattt tgctgtaatc caataatagc acattatgat agcgcaatca acagggaatt
    42661 taatttttta tcttttcgtt tgaccgtata gggtaatata acagttagat cacatcaact
    42721 agggtatatc tgcgtatact tactgctaaa ataagtatat cta
//
LOCUS       DACGLE010000017        40286 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0534
            SAMEA3304449-rid9560453.denovo.018, whole genome shotgun sequence.
ACCESSION   DACGLE010000017 DACGLE010000000
VERSION     DACGLE010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304449
            Sequence Read Archive: ERR985064
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 40286)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40286)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702225.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702225.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 20:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,045
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 27 of 67
            Pseudo Genes (incomplete)         :: 25 of 67
            Pseudo Genes (internal stop)      :: 19 of 67
            Pseudo Genes (multiple problems)  :: 4 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40286
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304449-rid9560453.denovo.018"
                     /strain="VREN0534"
                     /db_xref="taxon:1352"
     gene            complement(21..251)
                     /locus_tag="IXT20_001029"
     CDS             complement(21..251)
                     /locus_tag="IXT20_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4859493.1"
                     /translation="MMEEFIRKNISDEYADFYDQSSKKDKFQMDVSILAILAFSENKQ
                     PVTAKKETVFSEGKIKTRYILEVETNFKNRSE"
     gene            complement(687..1253)
                     /locus_tag="IXT20_001030"
     CDS             complement(687..1253)
                     /locus_tag="IXT20_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HAQ4859494.1"
                     /translation="MLQTALHFSHTLLQEILQPGDHVVDATMGNGHDTVFLAEHIGKT
                     GHVYSFDIQQQAIDATRERLEQRQLEERVSLFLQGHETLGEVIAEQQNLKAGIFNLGY
                     LPKSDKAIITMPETTRTAMEEILKRLVPRGRLILVVYYGHEGGEKELDMVDSFCSKLP
                     QETYNVLNYRFINQKNQPPILYCIEKKR"
     gene            complement(1253..2209)
                     /locus_tag="IXT20_001031"
     CDS             complement(1253..2209)
                     /locus_tag="IXT20_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390904.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HAQ4859495.1"
                     /translation="MTFPYSEGNKRYHSWNYALRNEFGGKIFKVPIDGGFDCPNRDGT
                     VAHGGCTFCSVSGSGDMIVAPEDPLPIQFQKEIDMMHQKWPHVQQYIVYFQNFTNTHA
                     PVEIIKERFEQVVNLPGVVGLSVGTRPDCLPDEVVDYLAELNERMYLWVELGLQTTFE
                     ETSKLINRAHDYQTYLDGVAKLRKHNIRVCTHLINGLPGESLEMMKENVRRTILDSDI
                     QGIKLHLMHLMRKTRMLRDYHEGRLQLMSRPDYVNVICDQLEMIPKEIIIHRLTGDAP
                     WDSLIGPMWSLKKWEVLNAIDEELLRRDSFQGKYDVRKKVSV"
     gene            complement(2209..2865)
                     /locus_tag="IXT20_001032"
     CDS             complement(2209..2865)
                     /locus_tag="IXT20_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HAQ4859496.1"
                     /translation="MKYKTSFQVAGSFFLVLFFILGWIVKFHLSWLSGFDQWFTGIVR
                     ASYPDLNSYQLFMTKFGNPLTVIILFACAALWMWYKKKRAETFWFVSGFIVIAGIINP
                     VIKLFFMRERPSLAHLVVETSYSFPSGHAAGSMILYGSLLFLLPAFVQKRTLRTLLQL
                     LMMFIIVSVGISRIYLGVHFPTDIIGGYLLSLSWLCLTYPVYREKRKQIDNSYRKKDF
                     "
     gene            3235..5253
                     /locus_tag="IXT20_001033"
     CDS             3235..5253
                     /locus_tag="IXT20_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAQ4859497.1"
                     /translation="MENIARKERRRLEQTKRFRKAKRGAAIVGTAMVGCSVAAPLVQP
                     VPVQADETPVQFGARINTAAFITEIATYAQPIAQANDLYASVMIAQAVVESGWGSSAL
                     SQAPYYNLFGIKGSYQGQTVYMDTLEFLNNQWVNKKEPFRQYPSFAESFNDNAYVLRN
                     TSFGNGYYYAGTWKSNTKSYTDATACLTGRYATDPGYAGKLNNIIATYGLTQYDTPAS
                     GNAGGGATTGGNNTGSAGSTGGSSNSGNTNSGSATSTTYTVQSGDSVWGISNKFGITM
                     DQLIQWNNIQNNFIYPGQKLTIKGSQENGSSTNNSGNNTNSSGNAGTSNGGQTTGAKY
                     TVQSGDSVWKISNDHGITMAQLIEWNNIKNNFVYPGQQLIVSNGGSSNAGANTGSNTG
                     NTSGSSSSSSSSSNTASGTKYTVKAGDSVWSVSNKYGITMAQLIQWNNIQNNFIYPGQ
                     QLTVSNSGSTNSTSGNTGSSTNTNNSSSVNTGSSSSQGTYTVKAGESVWSVANKNGIT
                     MDQLIEWNNIKNNFIYPGQQLIVKGGTSANTNTGSTTSAAKPNTPNTSATTSTSSTGN
                     TMYTVKAGESVWSVANKHHITMDQLIEWNNIKNNFIYPGQQVIVKKGSSQSTNQQSVT
                     GSKTYTVKSGESVWSVADSHGITMAQLIEWNNIKNNFIYPGQTLIVAK"
     gene            5680..8094
                     /locus_tag="IXT20_001034"
     CDS             5680..8094
                     /locus_tag="IXT20_001034"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016183651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HAQ4859498.1"
                     /translation="MSYNHKEIEKKWQKYWAKTNTFNTHDDPEKPKFYALDMFPYPSG
                     QGLHVGHPEGYTATDILSRVKRSQGYNVLHPMGWDAFGLPAEQYALDTGNDPAEFTQK
                     NIETFRRQINSLGFSYDWNREVNTTDPEYYKWTQWIFTKLYEKGLAYEAEVAVNWVPE
                     LGTVISNEEVIDGKSERGGYDVVRKPMRQWMLKITAYADRLLDDLELVDWPESIKEMQ
                     RNWIGRSVGANIEFKVAGTDKSYTVFTTRPDTLFGATYSVLAPELDLVREITTPEQKA
                     AVEAYIEETAKKSDLKRTDLAKEKTGVFTGAYAINPVNGKEIPIWIADYVLSSYGTGA
                     IMAVPAHDERDFEFAQTFGLEILPVIEGGDVEKAAYTEDGTHINSEFLDGMNKQEAID
                     KMNEWLEENGVGKKEVSYRLRDWLFSRQRYWGEPIPIIHWEDGTVTAVPEEELPLRLP
                     KTKNIKPSGTGESPLANIEEWVNVVDPVTGKKGRRETNTMPQWAGSSWYYLRYIDPHN
                     KKELADYEKLKRWLPVDIYIGGAEHAVLHLLYARFWHKFLYDIGVVPTKEPFQKLYNQ
                     GMILGENNEKMSKSRGNVVNPDDVVETYGADTLRMYEMFMGPLDASIAWSENGLEGSR
                     KFLDRVWRLIVDENNKMRDRITTLNDGKLDKVYHQTVKKVTEDYENLHFNTAISQLMV
                     FINEAYKVDALPYEYIEGFVQLLAPIAPHMGEELWAILGNDGGISYAPWPTYDEAALV
                     EDEVEVVFQVNGKVRAKSNVPRDLGKDELEKAALANEIVQEYIEGKTVRKVIAVPNKL
                     VNIVAN"
     gene            8442..9995
                     /locus_tag="IXT20_001035"
     CDS             8442..9995
                     /locus_tag="IXT20_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HAQ4859499.1"
                     /translation="MDQELFIIEVLSAKQKIIEKAKKRAKRIMFKLLSLVFFSGLLFF
                     GAVSFLYRHMSAVVRSAEGMQKLNNLYHFSWGVGGITIVVLIVFLLLIWLRYHQLSRH
                     FDQWLSQRAQQFLHNKPTSQDDDYLYFEKTSTSQAFRIQKSDCQLLLRSLDDLPIYLG
                     ESKSKFGMNTFHIFLLENEPRWEKSQSSPGGYLTTKMSTLVTGFFLILLAGTIYYGRY
                     TQPKMSSHINAASHMTMNSTNDTHIQKTNEGLLADPKQKVNLTTQKINDLKLDTETHS
                     LYMTTDSGANWVFVPIDSQWLRFGEYTLTTGLIPEGYWMDNTYDISADFSWFIYSSGD
                     DVRLLTSRDNGKTWQTSIITQSVQPIRYRKAVFYGNDGLFICTSASGMSAETLYIYTT
                     MDGGITWIKSGSTMINQPVQNVSFVTTSLGFVSTRESLFYTNNGGRSFKESVLSIPEN
                     YQTGGIDLFQSPNEVTQISANTLETKFYLLKKGPIDAGKMFACLFRSTDNGETWQFVQ
                     QLSQVEQGD"
     gene            complement(10376..12160)
                     /locus_tag="IXT20_001036"
     CDS             complement(10376..12160)
                     /locus_tag="IXT20_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ4859500.1"
                     /translation="MEKKKTLNTRALIRFWKMIKPEHKYFYGLFLCSLFGNLLVVAMP
                     LIMGIGIDDLLSEIRKTGIGQLSFSDIKQALLFPVLLLIGCSVLSSITSFIQERTMAA
                     LSEKITLRVRKEITKKFKTLPMSFFDNHQVGDIISRTTTGLNQLSQVLLTGINQFFTS
                     VVTIAAAGLMLFYIESKLTLLVLLLIVGSSIVTTKIANKNKQISEQNQYELGVLNNRM
                     EEILAGNLVVKTFNQQAHAEAAIHEVNKKQYNAFKRAQFMNCAIYPAIRFINQLAFII
                     SAVFGAMLVLSGGVTIGFLQAYLQYINQISEPISTASYVINSIQSAMAAIDRIFEILD
                     ETDEIPDLPDARLLEQPKGKIAFKNVQFGYTSDKILMNQVDFSVKPKQTVAIVGPTGA
                     GKTTLVNLLMRFYELNKGKIEFDGLDITKLSRGELRRSFGMVLQNTWLFEGTVADNIA
                     YGKMDATREQIIEAAEIAQCDHFIRTLPDGYDTIISSENGTLSQGQQQLLTIARVILA
                     NPPVVILDEATSSVDTRTEALIQQAMEAVTKDRTSFVIAHRLSTIENADMILVMKNGD
                     IVEKGTHSELLQSPTLYASLYNSQFQIA"
     gene            complement(12175..13896)
                     /locus_tag="IXT20_001037"
     CDS             complement(12175..13896)
                     /locus_tag="IXT20_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ4859501.1"
                     /translation="MSILKTFLQKNKGLALLTFVMICLEIAGTLGVPKLVAKLIDTGI
                     ASGEASVIKKIGVEMLLVAFLGTIAAVASSYFSALASARFGFQTREKYFRKFQQLSMK
                     DISRFSSGSLLTRMTNDVDNVQQMIVLFCQMILPAPVICVFTILMMFRYSLLLTWVTL
                     ISVVFYVWIVYRLMKRGAPLSLSIQPKMDRITITLREFFTGINMIRAFDNQSYEEKRT
                     NKSFADYANQMIKVNRIFAWVTPIAFLLMGIVYASILWFGGNLVAEGALQIGVVTAVV
                     EYSMLTLAYLMIAAMVLVIIPKSFASLRRIEEILQAEIEIEEETIKTVAEPTFDQTNA
                     AAFEHVTFKYTEISDPVLEDIDFTIPKGKTTAIVGGTGSGKSSLAKLLLRLSDPTIGK
                     VTLGGIPLTQMTQEQIRSHISYVPQKAFLFSGTILSNLQMGNEYASTEELSKAIKIAQ
                     LEELVDTLPDGLDSFVAQGGSNYSGGQKQRICIARALIKPAEIYVFDDSFSALDYKTD
                     AALRAALHKHMSEKTLVIVAQRLSTIQQADNIIVLDEGKIVGQGTHEELLSNCQTYQE
                     FAKSQGI"
     gene            13996..14757
                     /locus_tag="IXT20_001038"
     CDS             13996..14757
                     /locus_tag="IXT20_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAQ4859502.1"
                     /translation="MKKMYTIGQVSELFDLPKSTIRYWDEQGLIRSSRQEENDYRLFD
                     IDDIFMLYDIVFYRKLDIPIKQMKNLYGKTLTELYETLDETERRIHKELVVMKQKQKE
                     IREQKKQLKLMIDTNEEEFPVEEIPFDCMISTEFEDIVEIKKFLPNYSSFGMMSMPAS
                     SSQTMYGFFIDPSEVHLFTQDVIWEKKDTAVYRRFLLKSEMNHAERNNILEIRERMYE
                     KGWKTGEVIGQYLLTNTDENNIRTEYYHAWIEMKK"
     gene            15724..15992
                     /locus_tag="IXT20_001039"
                     /pseudo
     CDS             15724..15992
                     /locus_tag="IXT20_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720767.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS607 family transposase"
     gene            16664..19786
                     /locus_tag="IXT20_001040"
     CDS             16664..19786
                     /locus_tag="IXT20_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAQ4859503.1"
                     /translation="MRNKFHKLLSMFLVMLIVINQFGNITVLAESLAEQPERSLTIEV
                     DQEEAALGEQFQLRVRGTPDLLNALVIDKSEDLIEGEVKQLNDYERLITYSSQHSGKY
                     ELTARSGDIQSNTVSIEISVPEESTSESFNVPEESTSESFNIPEESLSESFNEARSSE
                     PSITQESQIERQEELLNYDIGISAERPTLPSRHTQTLKGYIQGGAGDILEEYGEMVVR
                     VPRASIGLIEFIRVEVADDSPLKPLAFYQEGTDVVFPFTFDYEGLNEAAQSYTFSITF
                     EVAFFQSGGTEPNNDFQVSLTYAERAANTEFSITPIPSSRPIFSKLYYGSREIDSQGN
                     LGNLLIDGQDVTNSNKFILQVNYEGSRKDGVVVTDVLPQGTTLAPNPTTWSSTGNREP
                     ISGGVRIAEVTNPGEANVRTHYVSEEFVDKVSFNKETNTLVVDFGNLAEGQSYFIEYA
                     LEVTDVDLFNEGRQEDGFSRNRASMTYNNQVMSRDYRLRIPESNLPAISLRKSVDKNV
                     INLNENELTYTLHVSMFSGTRNTLVIEDVLEEGMEFVSFLSDIPSFIDAEVNNGIVRF
                     SANNTIGGPTSFTLSFKVNVEAYAVGDQILNFATLVNSDNRLNTSTVRTRKIDGRIKV
                     IKTDENGQRLAGAKFEILSSDSQVIQEGITAENGEFLSEPLPVGSYQVRETEAPEGYL
                     LDATVHDVFINEYSIEEVTLTIENILDTGGVELIKHAEGSGEVLQGAVFELQNREGET
                     LQTGLTTGEDGKLAIDGLAPGAYQLVETQAPIGYELDATPIEFEIERSQTAVVELTKA
                     NRLTPGGVVLTKIDDQSGEVLQGAVFELQDANGTVLQSGLTTGADGKLAIDGLAPGVY
                     QLVETQAPTGYELDATPIEFEIERSQTAVVELTKENRLTPGGVVLTKIDDQSGEVLQG
                     AVFELQDANGTVLQSGLTTGADGKLAIDGLAPGAYQLVETQAPTGYELDATPVTFKIE
                     KEQEVAIFLTKENRKVADSSDIRGNNPTSSGNKHLPKTGETIISQFILSILGVLLVFI
                     SIKFRKKRNI"
     gene            complement(20210..20380)
                     /locus_tag="IXT20_001041"
     CDS             complement(20210..20380)
                     /locus_tag="IXT20_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAQ4859504.1"
                     /translation="MPRLTNYFLSCTNPKEIEFLVTDMNATYFQLTKHVLPNAKLVID
                     RLLGFSTPFKEA"
     gene            complement(20574..20753)
                     /locus_tag="IXT20_001042"
     CDS             complement(20574..20753)
                     /locus_tag="IXT20_001042"
                     /inference="COORDINATES: protein motif:HMM:NF026041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4859505.1"
                     /translation="MPMVIRLKKQRYTCKNCRSHWNAQSYFIRPRHSISNHVRHKITS
                     LLTEKVSLFFISKSC"
     gene            21140..22252
                     /locus_tag="IXT20_001043"
     CDS             21140..22252
                     /locus_tag="IXT20_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAQ4859506.1"
                     /translation="MTVKNKSKKKGRQQVDFYQSLQLDPFILKQKIREAASKKEKCMY
                     ICSLFLRSLFIVLFAICFIIIITTLFESKHKPYAVVLFCMLMSIRFVDFGYKISHSII
                     GLAIVMFSLLVAPYVQLIKWSVMGMLIHFILLSSILMATASDPKMGNASLYGFSYLFI
                     VYSLPKDSLNKNFFTQTSSLLFLFFCWFSVLLYRKHREKNKDKSLFKEIFLKGMYSQE
                     KIWMLIYAFGISLLIVAGEYVPFQRLMWAGFAFSSIVSSYGLMSIGFKERAVDRIIGS
                     LIGCVLFIGISQFIPFNWVGILGGLALGVCSTYRYKTVFNSFGALAITASLFGVPGAV
                     TIRIFENILGVCLGIMYIGVTEILIRKIREKHGLNH"
     gene            complement(22330..22623)
                     /locus_tag="IXT20_001044"
     CDS             complement(22330..22623)
                     /locus_tag="IXT20_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HAQ4859507.1"
                     /translation="MSITVNILYTSTNGQARKFAEEMVERNIVKAIRAEEGNEKYDYY
                     FPLEDPESLLLIDRWKDEEAIEKHHKSEMMAQINELRKKYKLKMKVERFTDLP"
     gene            22791..23105
                     /locus_tag="IXT20_001045"
     CDS             22791..23105
                     /locus_tag="IXT20_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIB"
                     /protein_id="HAQ4859508.1"
                     /translation="MKKALIICAAGMSSSMMASKTTDFFKQKREEVLVDAVSATEGDN
                     MIKNSDFDLFLISPQTTMFLDKFKKLGESVGKPVVSIPFQAYVPIPTGIQKLAEVIEE
                     NI"
     gene            23126..25104
                     /locus_tag="IXT20_001046"
                     /pseudo
     CDS             23126..25104
                     /locus_tag="IXT20_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345663.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
     gene            25117..25443
                     /locus_tag="IXT20_001047"
     CDS             25117..25443
                     /locus_tag="IXT20_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIA"
                     /protein_id="HAQ4859509.1"
                     /translation="MAEKINSETLQVTSFDIIFHSGNARTLIHEAFRSMRKEMFEEVE
                     EKLRQANEEILHAHQSQTQLLKEYASGTKIEMEIIMVHAQDHLMTTMTLLEVAQEMNY
                     LYQKNK"
     gene            25459..25977
                     /locus_tag="IXT20_001048"
     CDS             25459..25977
                     /locus_tag="IXT20_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4859510.1"
                     /translation="MQDTVKRKIELEKKQLSLKNMYFNRYLIVRYLTAFFFFMNMQWM
                     ILLLSAKSLGSSLPMILILAILPAVGEQVKLYRKHQTNVPWTKWYFLFQSVFNLLVIP
                     VLFTSGFTFLYPFMANNNGGQLFVFILVVSGIFASAFIQYRLKKISLNQDQQYIRIKQ
                     YEKALYLGKENN"
     gene            25977..27329
                     /gene="celB"
                     /locus_tag="IXT20_001049"
     CDS             25977..27329
                     /gene="celB"
                     /locus_tag="IXT20_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HAQ4859511.1"
                     /translation="MFDFLQKYLMGPMGKIAQLKIVRAVMAAGMASIPFTIVGSMFLV
                     LNILPLPFPFLEGFFNATFFKVSDLYMIVNTMTMGILSIYFAIVFAYELTSIERDEQK
                     LNVNPLTGALLSVFAFFMCIPELIMTDGKISLIASINETETVVNGIRMGSFVERLGTS
                     GIFTAIIMSIIAVELYCMCVKRNWVIKMPDVVPPGVSRSFTALIPTFVIAFVVMIING
                     LLVALGTDIFKMIAIPFGFVTQLTNTWLGVMVIYFLIHALWIVGIHGANIITSFLTPI
                     VLANMAANAQGANYPLAGEFNNSYVTVGGSGATLGLIIFIAFLAKSDQLKVLGKASLV
                     PGLFNINEPIIFGMPIVYNPYLAVPFFLAPMASATLAYFAIRLEIVKPMLAQMPWPSP
                     VGIGAFVGSGGDWKAAVLAVICAIIAFIIWFPFIKFYDNKLLNEEREKAAELASEKLK
                     "
     gene            complement(27413..28918)
                     /locus_tag="IXT20_001050"
     CDS             complement(27413..28918)
                     /locus_tag="IXT20_001050"
                     /EC_number="6.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L-
                     lysine ligase"
                     /protein_id="HAQ4859512.1"
                     /translation="MSFSLEDIRHLLLKENLLKEFVSTRGWHLDVPEKAEFLQLSYDS
                     RKADASTLFFCKGMNFKEEYLDSAIEQGIQYYVSEQPYENSAVGIIVTDIRKAMALLA
                     MAFYDYPQNKLKVIGFTGTKGKTTAAYFTKAILDHTTNKKTALLSTMNTTLDGKTYFK
                     SHLTTPESLDLYRMMSEAVENGMTHLVMEVSSQAYKTQRVYGLTLDVGIFLNISPDHI
                     SPIEHPTFDDYFYCKRQLILNSKTVVLNHESDYFDLLRETADLFQIPTITYGRSEESN
                     YQVIRSDKGTHGFTLSSHEDRLAICDTAYDILLAGGFNQENAASAIIAAALTGASSQD
                     AQDGLKEARVPGRMDQLIQSNGAHVYVDYAHNYLSLKTLLKFAKNEHPDGRVIVVLGS
                     PGNKAISRRHDFGKVLSETADIAFLTADDPAFEDPKKIAEEINEAITNPDLIVHYEMD
                     RPEAIRRALAESAPQDSVVIAGKGVDPYQKINGVDEPYEGDYAIVKRLIEE"
     gene            complement(29010..29600)
                     /locus_tag="IXT20_001051"
     CDS             complement(29010..29600)
                     /locus_tag="IXT20_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAQ4859513.1"
                     /translation="MTQEIKKLPVIFLGLFFITVGLNWFLLPHDIASAGVGAIGHLVE
                     TAFSIQRSVTIWSINLMMLLLAAVLLGKSVFFKTVAGSLLFPVVLEIVPKVEMISSHF
                     FSLISGSLLFSLGVYTLYTVGASNGGITIPPIIFHKFFRLPMPQGLLLTNSLIVFLNY
                     YVFGLLETLFVLLSITVSSLFMKILIRLSPVSRKFE"
     gene            29779..31425
                     /locus_tag="IXT20_001052"
     CDS             29779..31425
                     /locus_tag="IXT20_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAQ4859514.1"
                     /translation="MDNQRKPALDQLTPQEKMARGSAWMTASNIISRLLGAVYIIPWY
                     AWMGENAKAANGLFNMGYNIYALFLLISTAGIPAAIAKQTARYNSLNEYGTSRRLFIR
                     ALQMMGGLGLLFALFMYIASPWLAHASGGGEELIPTMRSLSIAVLVFPCMSVIRGYLQ
                     GNQEMMPYALSQIVEQVARIFYMLLATFIIMKVMDGDYVTAVTQSTFAAFIGMLASIL
                     VLLYFLKKHQAYTSAFIHYSENKINIATKELLLDTVKEAIPFIIVGSGVTIFKLVDQF
                     TFMKIMSASTEYSNAQLLDLFSIFSANPDKLTMVVIALATSIASTGLPLITEAVTLKD
                     RVGLAKLTSGNLQLFSFFMFPATFGVMLLAYPLNTLFYTPDSLGSNVLIQASFVGLFL
                     GLYMLVSNMLQGMFENKAAINYLVIGFLVKLLLQYPAIRLFEVYGPLLATMIGFAVSC
                     TLILKRIHEVARFKPSFVWRRTLLIFILTLIMLLASWITKMIFGAFLNEDSKFQSLLL
                     ILVVAGIGALVYGYLALKIRLAERLLGPGIARLRHKLKIK"
     gene            31474..32193
                     /locus_tag="IXT20_001053"
     CDS             31474..32193
                     /locus_tag="IXT20_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HAQ4859515.1"
                     /translation="MRLDKFLADMGLGSRKEVKGLIKKGTIEVNGQVVKSDKAQVNEF
                     EDAVTYLGEIITYQKDFYYILHKPAGVISATQDNHDQTVIDLLEDQDYREDLFPVGRL
                     DKDTEGLLILTNDGVFAHQLLSPKKHVEKEYLAEVSGIMTEEDIRLFAEGLIIDGDEQ
                     TLPAKLQIDQVDQETETSIIRLILHEGKFHQVKRMVKAVGKEVLYLKRIRMGKFWLPK
                     DLNKGQYRPMTKEELEQVREK"
     gene            32672..33619
                     /locus_tag="IXT20_001054"
     CDS             32672..33619
                     /locus_tag="IXT20_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ4859516.1"
                     /translation="MKKRKGFNHFFMNLWRYKALVLMAVPGMLWMIFFFYIPVMANVV
                     AFKNFHISADGFLASLRESPWVGWENFKFLFSSDQAFLITKNTILYNVAFILLNLLIS
                     VVFAIIMSELRNKRLVKVYQTMSLLPYFLSWVIISYFVYAFLSPDKGIFNQWITGHGG
                     EAVNWYNEPKYWPFILVFIGTWKGIGYNSIIYFASVMGIDPTYYEAAMVDGASKWQQI
                     KHVTIPQLIPLMTILTILAVGNIFRADFGLFYNIPRNSGALYDVTQVLDTYIYNGLTS
                     TGDFGMTAAAGLYQSVVGFVLLMITNTIARRFDSDSALF"
     gene            33631..34551
                     /locus_tag="IXT20_001055"
     CDS             33631..34551
                     /locus_tag="IXT20_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAQ4859517.1"
                     /translation="MKKKKVERVQVRSFNKTANFFSNIMIALFAISCILPFIFVIAIS
                     FTDESILSTHGYSFWPEKFSIFGYTYLFDQMQDKIFQALFVTVLVTVLGTLINSTATS
                     LYAYAISRSNFPFRRFFTVFCLITMLFSPGMVANYLVMTNLLQLKDTIWALILPMAVS
                     PFNIIVMRTFFKRSVSDSIIESARIDGASELRIFVQIVLPLAVPGIATISLFAALGYW
                     NDWFNALLYIQDDKLVPLQYLLMKIQSNIQYLTQNSGAGSQLTGGLASVPGESARMAI
                     VVISTLPIAISYPFFQKHFVKGLTIGGVKE"
     gene            34592..36049
                     /locus_tag="IXT20_001056"
     CDS             34592..36049
                     /locus_tag="IXT20_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAQ4859518.1"
                     /translation="MKLWKKLAFTGVSALLLGTLAACGGSGSKDQAEASNSDTLQMYQ
                     IGDKPDNFDQLMEVANKRIEKEIGVKVNINYIGWGDYEKKMNVIISSGENYDIAFANN
                     YVSNAQKGAFADLTELAPKYAEKAYNDLDEAYIKGNLVNGKLYAFPVNGNVFAQQVLT
                     FNKALLDKYDLSIDGIQSYADAEKVLKEFHEKEPNTAAFAIGQGFKVQGNFDYPLGNT
                     LPFAIDLDGDDSKIINQYDNETYIGLLRTMHSWYKQGLIPSDAATSNKDYPLEGNTWL
                     MRGETQGPYDYGDTILTNAAKQELVSKAITVPLKSTAQAQMANFVVSNTSKNKEKSVE
                     LLGLLNSDPELLNGLVWGIEGEAWEKVDGSDDKIKLLDGYQPNTHMSAWNTGNNKILY
                     TQESITDDMIAKRDQSIADAETSPILGFSFNTDSVKTELSNISNVMNQYLDGLNTGTV
                     DPDETLPKLKDALDKAGYDKVLKEMQKQYDEFRQE"
     gene            36394..37008
                     /locus_tag="IXT20_001057"
     CDS             36394..37008
                     /locus_tag="IXT20_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HAQ4859519.1"
                     /translation="MLGKVLEELFIRIWVIIKLTLYFWIYTFAGGIIFGLGAAWKTVN
                     ELFYLYGFEYKEITIKRGWNIYKRNFLRGNLLFSLFLSGTALLSYNLYLSVQIKGLIF
                     LAIDFILLFALLCLFATYQYSMILDSEYTISVPNLLKLSFISVFSSFSSFLKIIIGSG
                     IILGVTWQFKGLILFGVIGLLTVWNGTMTTHWREELDKQLESYE"
     gene            37001..38725
                     /locus_tag="IXT20_001058"
     CDS             37001..38725
                     /locus_tag="IXT20_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAQ4859520.1"
                     /translation="MNKFRILFSKGTLLNRLLRKYAVIMIGVTMTATVIFSIHTWEQN
                     QKQAENMTSDAVQSTSRMLNDKTTLSRIIKNQLVGNSEKIENVTTYLTKPIDQYLMYV
                     YEQQNSTDELVSFPNQIKDLYANYEELSAIYIVLNQLPEYYESSRENKGGEIKKGTPK
                     LLDAFYIKLPITQGGAESGSIFIGFQKKELDMILENLTSFNGLSLYMISGTTNRLYTF
                     HDNKIAKETFERQEMIITESLKKNSQLPIDDLEKNNFVQHQELSDDYRILAVLDKKSV
                     KIETGKNLAPLIIGIIILDNILLIFLYKTFKRYSQQVSIVMQAMDQTAAGNLETRIDI
                     TNTEYELKELSIGINEMLDSINQFVEDIYKLEIKQQDAHMRALQAQINPHFLYNTLEY
                     IRMYAISEGSEELADVVYAFSALLRNNTNQEKTITLKEELDFCEKYVYLYQMRYPNRV
                     AYHFMIDPDLEKIEVPKFVIQPLVENYFKHGIDFTRFDNALSVKVLQEGKRVRIIIKD
                     NGKGMTEKRLKQIEEKLSHPKVELHGSIGLQNVNERLRASFGSSYYMSLENNETGGLT
                     VSITFKEG"
     gene            38722..40173
                     /locus_tag="IXT20_001059"
     CDS             38722..40173
                     /locus_tag="IXT20_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ4859521.1"
                     /translation="MSVYQVLLVDDEYMILNGLKKIIDWQSLGFQIVATAENAKEGLA
                     VLEQRQIDLVVTDVTMPEINGLEFIEAAQKERHNFEFMILSGYQEFDYLKGGMQLGAV
                     NYLMKPVNKFELVESLKKIKTRLDQQNEQKNQQEIYQELLFSQWLNEELDEISEEEII
                     GQISEKQHRIVLIQLARTHDTLINRWLQERQQIFYYQRNYGDRVLITLLLEADEVDGF
                     CCFVQENFPDQEWLISIGEETDSLDKIPETFQQAKDNLQLHQFYGDKDEHVFYAVQAE
                     TKDQTIDFSSFRRVLQNKQLEVAQKMITDFFEQFQLAAMMPEDIRYSSFLLFMEIQRE
                     LIDLEDEEYLQGIEKIHQAKTVQELQQLLLSFIKKHQRQKKYSDNVEKVIVILHQHYQ
                     EPLTLKEVSENLHLNVMYLGQLFKKETKKSFSAYLNHLRMEKAKQLLLHSNQNINEIA
                     SEIGYNNTTYFSKLFKKIVGRSPKEYRENSGES"
BASE COUNT        13104 a         6976 c         8123 g        12083 t
ORIGIN      
        1 ctcattaaaa agcataacca ttactccgat ctgtttttaa aattagtttc tacctctaat
       61 atatatcgag tttttatttt gccttcagag aatactgttt ctttttttgc agttacaggt
      121 tgtttatttt cggaaaaagc taatatagct aagattgaaa catccatctg gaatttatct
      181 tttttactgc tttgatcata aaagtctgca tattcatcac tgatattttt tctaataaat
      241 tcttccatca taaaaatcac ctcaaaacga ttatacgaac ttacgttctt gttgtaaagc
      301 gatatttgag gggcaaaaaa ggggcataat ttgactgata ttaagttagc tattgatata
      361 tgaatgacat acaaaaatat aaaatctttt aaaaatgctt atatatcaat gtttttgcca
      421 ttcttttata tatctgttat atgtcaaaaa aatgtgttca atttcaattt gtatttgaat
      481 aaagaggttg tgaaaaagct gtcctgcatc aagtaataag aacagccaat caaaaacagc
      541 ttctcatgtt tttgatttgg ttggacttat taccgcagat ataagctttt gagcaccgtg
      601 aataaagagg ttatgaaatc aacgctctaa cctgagtatt agagaaaaaa acgcaaaaca
      661 tatcatttat gttttgcgtt ttttatttat ctttttttct caatacaata taaaataggc
      721 ggctggttct tttggttgat aaaccgataa ttcagcacat tatatgtttc ctgtggcagt
      781 ttggaacaaa aagaatcaac catatccagt tctttttctc caccttcatg accatagtag
      841 acgacaagga tcaatcgtcc gcgaggcaca agtcgtttca atatttcttc catagctgtg
      901 cgtgtagtct ctggcatagt gatgattgct ttatcacttt ttgggagata ccctagattg
      961 aaaatccctg ctttgagatt ttgttgttca gctatcactt ctcctaacgt ttcgtggcct
     1021 tgtagaaaca atgagacacg ttcttctaac tgacgctgtt ctagcctttc acgagtagca
     1081 tcgattgctt gttgctggat atcaaaacta tagacatgac cagtttttcc gatgtgttct
     1141 gctagaaaaa ccgtatcgtg gccattgccc atcgttgcat ctacgacgtg atcccctggt
     1201 tgcaagattt cttgcagtaa tgtatggctg aaatgcaaag cagtttgcaa catcagacgc
     1261 tcaccttttt ccgtacatca tattttcctt ggaagctatc tctgcgaaga agttcttcat
     1321 caattgcatt caagacttcc cattttttca agctccacat tggtccaatc aaggaatccc
     1381 acggcgcatc ccctgtcaaa cgatggatga tgatctcttt tggtatcatt tccagctggt
     1441 cgcaaatgac attgacatag tcaggacggc tcattaattg aaggcgacct tcatgatagt
     1501 cccgcaacat tctcgttttg cgcatcagat gcatcaaatg gagcttgatt ccttggatat
     1561 ctgaatctaa aatggtgcgt ctgacatttt ctttcatcat ttctaaactt tcgcctggta
     1621 atccgttgat caaatgcgta caaacacgga tattatgttt gcgtaattta gccacgccat
     1681 ctaaataagt ttgataatca tgtgctcggt tgatcagttt tgaagtttct tcaaaagttg
     1741 tttgcaatcc aagttctacc cataaataca tccgctcgtt cagttcagca aggtaatcaa
     1801 ctacttcatc tggcagacaa tccggtcgtg taccgactga taatcctacg acacctggta
     1861 aattgactac ttgctcaaat cgttctttga ttatttcaac tggcgcatgg gtgtttgtaa
     1921 aattttggaa ataaacgatg tattgttgga catgaggcca tttttgatgc atcatgtcga
     1981 tttctttctg gaattggatt ggaagagggt cttcaggtgc aacgatcatg tctcccgaac
     2041 cagatacact gcaaaaagta caaccgccat gggcaacagt cccatctcga ttcggacagt
     2101 caaatcctcc atcgatcggc accttgaaaa ttttcccgcc aaactcattt cgtaaagcgt
     2161 aattccaaga atgatatctt ttatttcctt ctgaataagg aaaagtcatt aaaagtcctt
     2221 ctttctgtaa gagttatcta tttgttttct tttttctcta tacactggat aagtcaggca
     2281 cagccaagaa agactgagta agtagccgcc gatgatatca gtaggaaaat gaacgcccag
     2341 ataaatgcgg cttatcccga cacttactat aataaacatc atcagcaatt gaagcaatgt
     2401 gcgtaaggtt cttttttgaa caaaagctgg aagcagaaat agtaaagaac cgtaaagaat
     2461 catactgcct gctgcatgtc cactagggaa actgtaactt gtttcaacta ctaagtgcgc
     2521 caatgaagga cgctctcgca tgaaaaatag tttgatcacc gggttgatga ttccagcaat
     2581 tacaataaat ccagaaacga accaaaatgt ttctgccctt tttttcttat accacatcca
     2641 taaagccgca caagcaaata aaatgatgac ggtaagagga ttaccgaatt tagtcataaa
     2701 gagttggtag ctgttcagat caggataaga agctctgaca atgcctgtaa accactgatc
     2761 gaaaccagaa agccacgaca gatggaactt tacgatccag cccaaaatga aaaacaggac
     2821 aagaaagaag ctaccagcca cctgaaatga tgttttatat ttcatacaat tcctcctttg
     2881 aagcaatgac ttattatagc atttttggtc cagcgaaaaa aacggtaaag agataaaacc
     2941 aatgtatgat tcctctttga ataaccaata ttcttataaa aaaaataact gtaatcggaa
     3001 agacattttt tggtaatgca aatgaaatgt tagggaaata aaatcagtgt ttttcgaaat
     3061 ccttttaaac aagaacgtag acgtaggaag aacttgaaaa tgaacagaag ttgttcatta
     3121 cggtaaatta caatatgatt acaaaaaaca acaaagctct taaataactt ttaataaata
     3181 ttaagagaag ttataattat taataataga taaattgaag ttggaggata gaagatggaa
     3241 aacattgcaa gaaaagaacg gagacgttta gaacagacca aacgttttcg aaaagcaaaa
     3301 cgaggggcag caatcgtggg aactgcgatg gtaggctgtt ctgtcgctgc cccattagta
     3361 cagccggttc ctgttcaagc ggatgaaaca cctgttcagt tcggcgcaag aatcaataca
     3421 gcggcattta tcacagaaat tgctacttat gctcaaccaa tcgctcaagc caacgatcta
     3481 tatgcatcag tgatgatcgc acaagcagtt gtagaaagcg gttggggcag tagtgcgtta
     3541 tctcaagcac cttattataa tctttttggg ataaaaggca gctatcaagg acaaaccgtc
     3601 tacatggata cgcttgaatt tttaaataat caatgggtaa ataaaaagga gcctttccga
     3661 caatatcctt cttttgctga atctttcaat gacaatgcct atgtacttag aaatacttca
     3721 tttggcaacg gttattacta tgccggtact tggaagagca acacgaaatc ctatacagat
     3781 gcgacagctt gtttgacagg gcgttatgcg actgatcctg gttacgcagg caaattaaat
     3841 aatatcatcg caacttacgg attgacccaa tatgatacac cagctagcgg aaacgcaggc
     3901 ggtggagcga ctacaggtgg caataatact ggaagtgcag gtagtactgg cggttcaagc
     3961 aattctggta atacaaactc aggttcagca acttcgacca cttatactgt ccaatctggt
     4021 gattctgttt ggggaatctc aaataaattc ggtatcacaa tggatcaatt gatccaatgg
     4081 aacaacatcc aaaacaattt catttatcca ggacaaaaac tgacaatcaa aggtagccag
     4141 gaaaatggct cttcgacgaa taattccggt aacaatacca actcttcagg aaatgctggt
     4201 acatcaaacg gaggacaaac aactggtgcg aaatacactg ttcaatctgg tgattctgtt
     4261 tggaagatct caaatgatca cggaattacg atggctcaat tgattgaatg gaacaatatc
     4321 aagaacaatt tcgtttatcc aggacaacag ctaatcgtat caaatggcgg ttcttctaat
     4381 gctggagcaa atactggctc aaatactggg aatacaagtg gctctagttc gtccagctca
     4441 agttcttcta atacagcttc aggaacgaaa tatacagtta aagccggaga ctctgtttgg
     4501 agcgtctcaa ataaatacgg aattacgatg gctcagctaa tccaatggaa caatatccaa
     4561 aataacttta tctacccagg acaacaactg actgtttcaa acagtggatc aacaaacagc
     4621 acgtcaggta atacaggttc gtcaaccaat acaaataaca gcagttcagt taatactggc
     4681 agcagttcgt ctcaaggcac ttataccgtg aaagcaggtg aatctgtttg gagcgtggca
     4741 aataaaaacg gcatcacgat ggatcagtta atcgaatgga ataatatcaa aaacaacttc
     4801 atttatccag gacagcaatt gatcgttaaa ggtggaactt cggcaaatac aaatacaggt
     4861 tcaactactt ctgcagcgaa accaaataca ccgaatacgt ctgctacgac aagtacatca
     4921 tccactggta atacgatgta taccgtgaaa gcgggcgagt cagtttggag cgtagcgaac
     4981 aaacatcata tcacgatgga tcagttaatc gaatggaata atatcaaaaa taatttcatc
     5041 tatccaggac agcaagtgat tgtgaagaaa ggttcatctc aatcaacaaa ccaacaatca
     5101 gttactggtt cgaaaaccta tactgtgaaa tctggagaat ctgtttggag tgttgctgac
     5161 agtcatggaa ttacaatggc tcaactaatc gaatggaaca atattaagaa caactttatt
     5221 tatccaggtc aaacgttgat tgtagctaaa taaaatagct gttcgaagaa ataacgattg
     5281 aattccacaa aaatatttgt tactataagg ctacttaaat atgacaacac ttgaaaagga
     5341 agtagtaaaa agactgaagt ttcagagagc gcatggctgg tgagaatgtg cacggatgct
     5401 tttgaactcg cctttgagtt gcccttgctg aatacccgag taagcaagcg gcggtgacgg
     5461 ccgttacaac gttcgaggcc ttttcactaa ggtaaaatta ggtggtaccg cgtagattac
     5521 gtcctataaa aagagcaatc tttttatagg atttttttat ttcttttgtg aagtttgaga
     5581 aaacaaagca aaaggaaata aaaataaaca gattctgccg gttttttcgt tatatttata
     5641 atagaaacaa gtgttttcaa tagaacagga ggacagcaaa tgagttacaa tcataaagaa
     5701 attgagaaaa aatggcaaaa atactgggca aaaacgaata ctttcaacac ccatgatgat
     5761 cctgaaaagc caaaatttta cgcactggat atgtttccct atccatccgg tcaagggcta
     5821 catgtagggc atcctgaagg ttatacggca acggatatcc tttcacgtgt gaaaagaagt
     5881 caaggataca acgtgctgca tccaatgggg tgggatgcat tcggtctgcc tgctgaacaa
     5941 tatgcgctag atacaggaaa cgatccagct gagtttaccc aaaaaaatat cgaaacattc
     6001 cgacgccaaa tcaattcttt aggatttagt tatgactgga atcgcgaagt gaatacaact
     6061 gatccggagt attataaatg gacacaatgg atctttacga agctttatga aaaaggtcta
     6121 gcttatgaag cagaagtagc ggtaaactgg gtacctgaat taggaacagt tatttcaaat
     6181 gaagaagtga tcgatgggaa aagtgaacgt ggtggctatg atgtagtcag aaaaccaatg
     6241 cgccaatgga tgctgaaaat cactgcttat gctgatcgct tattggatga tctagaatta
     6301 gttgattggc cagaaagcat taaagaaatg caaagaaatt ggatcggtcg ttcagtagga
     6361 gcaaatattg aatttaaagt ggctggtacg gataaatcct ataccgtatt tacgacacga
     6421 ccagatactc tattcggtgc aacttactcg gttcttgcac ctgaactaga tcttgttaga
     6481 gaaattacga cccctgaaca aaaagctgct gtagaagctt atatcgaaga gacagctaaa
     6541 aaatcagatc taaagagaac ggatcttgca aaagaaaaaa caggagtatt tactggtgct
     6601 tatgcaatca atccagttaa tggcaaggaa atcccaatct ggatcgccga ttatgtatta
     6661 tcttcatacg gcacaggagc catcatggct gttccagcac atgatgaacg tgactttgaa
     6721 ttcgctcaaa ctttcggact agaaattctt ccagttatcg aaggtggaga tgttgaaaaa
     6781 gctgcttaca cagaagatgg tacgcacatc aactctgaat ttctagacgg catgaacaaa
     6841 caagaagcaa tcgataaaat gaatgaatgg ctagaagaaa atggcgtagg gaaaaaagaa
     6901 gtcagctatc gcttacgtga ctggctattc tcccgtcaac gttactgggg tgaaccaatc
     6961 ccaatcatcc attgggagga cggaacagtc acagctgtcc cagaagaaga gctaccatta
     7021 cggttgccta agacaaaaaa catcaagcca agcggtactg gagaatctcc attagcaaat
     7081 atcgaggagt gggtcaatgt cgttgatcct gtaacaggta aaaaaggccg tcgcgaaacg
     7141 aatacaatgc cacaatgggc aggaagctca tggtattacc tgcgttatat cgaccctcat
     7201 aacaaaaaag aactagcaga ttatgaaaag ttgaagcgtt ggctaccagt tgacatctac
     7261 attggtggag cagaacatgc ggtacttcat ttactatatg cacgattctg gcataaattc
     7321 ttatacgata tcggtgttgt tcctacaaaa gaaccattcc aaaaactata caatcaagga
     7381 atgatcttag gcgaaaacaa cgaaaaaatg tcaaaatctc gtggtaatgt agtcaatcct
     7441 gatgatgtgg tcgaaactta cggtgcagat acactgcgca tgtacgaaat gttcatgggg
     7501 ccattagatg cttcgattgc ttggagcgaa aatggcttag aaggaagccg taaattcttg
     7561 gatcgtgtat ggcgtttgat cgttgatgag aacaataaaa tgcgtgaccg tatcactaca
     7621 ttgaatgatg gcaagcttga taaagtgtat catcaaacag tgaaaaaagt gacagaagac
     7681 tatgaaaatc ttcatttcaa tacagcaatc tcccaattga tggtctttat caatgaagcc
     7741 tataaagtgg acgctctgcc ttatgaatat atcgaaggat ttgttcaact actcgcaccg
     7801 attgctcctc acatgggtga agaactatgg gcaatccttg gaaatgatgg tggtatttct
     7861 tacgcaccat ggccaacata cgatgaagca gcacttgttg aagacgaagt agaagttgtt
     7921 ttccaagtaa acggtaaagt tcgtgcgaaa agcaatgttc cccgagactt aggaaaagat
     7981 gagctagaaa aagcagcatt agccaatgaa atagtgcaag aatatattga aggcaaaaca
     8041 gtcagaaaag ttattgcggt accaaacaag ttagtcaata tcgttgctaa ttaattggct
     8101 ctttgtcaat aaggactgat gagttgtgca aaatcaaatc tgggccagaa tgaacttcat
     8161 tctgtctcag attttttgtt atttcaaatt gattaattga ttgatcaaaa aggttttgat
     8221 tttcatgttc tcaaacacgt tcaatgtctt tttcttctga atggtcttgt aagagatctc
     8281 tgtaatcatc aaatttttgt ctattaatct tagtattttt tgatgtataa actacctgaa
     8341 attgagagta cataccaaat tcataaattt tttattagtg ggactagtga ttgtatggat
     8401 tttgacgcat aatgaatgaa gtatgagaaa aggagagaca aatggatcaa gaactattta
     8461 ttatcgaagt attatcagct aaacaaaaaa ttatcgagaa agcgaaaaaa agagcgaaaa
     8521 gaataatgtt caagctttta agcttagtat ttttctctgg tttgcttttt ttcggagctg
     8581 tatcgttcct atatcgccat atgagtgcag tagtccgttc agctgaaggg atgcaaaaat
     8641 taaataattt gtatcacttt tcgtggggag ttggcggaat aacaattgtt gtgcttattg
     8701 tttttcttct acttatttgg ctccgttacc atcaattgtc gcgtcatttc gatcaatggc
     8761 ttagtcaaag agcacaacag tttttacata ataaaccaac ttctcaagat gatgactatc
     8821 tttattttga gaagacttcg acgagtcaag cttttcggat acaaaaaagt gattgccagt
     8881 tattgctgag atcacttgat gatttaccta tctacttagg cgaaagcaaa tcaaaattcg
     8941 gaatgaacac attccatatt tttttactag aaaacgaacc cagatgggaa aaaagccagt
     9001 cttcgcctgg tggatacctg acaactaaga tgagtacatt agtcactggt tttttcctca
     9061 ttcttttagc tggaacaatc tattatggga gatacactca gccaaaaatg tcttctcata
     9121 taaatgctgc ttctcacatg acaatgaact caactaacga tacgcatata caaaagacaa
     9181 atgaagggtt gttagctgat cctaagcaaa aggtaaattt gacaacacaa aagataaatg
     9241 atctcaaact tgatacagag acgcactctc tctatatgac aacagatagt ggtgctaatt
     9301 gggtatttgt gccgattgat tcgcaatggt tgcgttttgg cgaatacacc ctgacaacgg
     9361 ggctgattcc tgaaggttat tggatggaca acacatatga tatttccgcc gatttctcat
     9421 ggtttatcta ttcttctggg gatgatgtac gtctgctgac ttcaagagat aatggtaaaa
     9481 catggcaaac gtctattatc acccaatcag ttcagccgat acgttatcga aaagcggtat
     9541 tttatggcaa cgatggtcta tttatctgta catcagccag tgggatgtct gcagaaactt
     9601 tgtacattta cactactatg gatggcggaa tcacttggat aaaaagtgga agtacaatga
     9661 tcaatcagcc agtacaaaat gtcagcttcg ttacaacttc tttaggtttt gtttcgacac
     9721 gtgaaagttt gttttataca aacaacggag gacgctcatt caaagaatca gttctgtcga
     9781 ttccggaaaa ctatcagaca ggggggatag atctttttca atcaccaaat gaagttactc
     9841 aaataagcgc gaatacgtta gagacaaaat tttatttgtt gaaaaaagga ccaatcgatg
     9901 cagggaaaat gtttgcctgc ctcttccgtt caactgataa cggggaaaca tggcaattcg
     9961 ttcagcaact atcgcaagta gaacaaggag attaaagaag agagattgtg agcctgacgt
    10021 tcagcaccga gtcacaagaa acaaattcaa aaaacagctt ctcatatttg ttgaatagtg
    10081 taactcgtga ttcggagttg acaagagcaa caagaagcga ttttgaagta tagcttctcg
    10141 tattttacaa gatcgtgtct tattgccgat aggttgtctc ttgaacaccg tttattcgta
    10201 aaaagggatt gtgacaagtc ttttgtcact acttaccgaa cgaaactgaa ggagctatcc
    10261 aaaaagtatc attagtatta ttctttgtgt ttctagtggc accactagta catttaaaaa
    10321 agctatcgat catggactaa tcatacatga tcgatagctt tttagataat gtttgttacg
    10381 ctatctgaaa ttgactatta taaagactgg cataaagcgt tggtgattgg agtagttcag
    10441 aatgtgttcc tttttcgacg atatcgccat tcttcattac aaggatcatg tctgcatttt
    10501 caatcgttga caaacggtgt gcaatcacaa aacttgtcct atcttttgtc acagcttcca
    10561 ttgcttgctg aatcaatgct tctgttcgtg tatctacgct agaagttgct tcatctaaaa
    10621 taacgacagg aggattcgct agaatgactc gagcaattgt caataattgt tgctgtcctt
    10681 gagataacgt gccgttttcg ctagaaatga tcgtatcata tccgtctggc agtgttcgaa
    10741 taaagtgatc acattgtgcg atttcagccg cttcgataat ttgttctctt gtggcatcca
    10801 ttttcccgta ggcgatatta tcagctaccg ttccttcaaa cagccaggta ttttgtaaaa
    10861 ccatcccaaa agaacgacgt agttctccac gtgaaagctt ggtaatatca agaccgtcga
    10921 attcgatctt tcctttattc agttcataaa aacgcatcag cagattgact aaagtcgttt
    10981 ttcctgcacc agttggacca acgatcgcta ccgtttgttt tggctttact gaaaaatcga
    11041 cttgattcat caatatttta tctgatgtgt aaccaaactg tacatttttg aaagctattt
    11101 ttcctttcgg ttgttctaaa agtcttgcat ctggaaggtc tggaatttca tctgtttcat
    11161 ctaatatctc aaatatccta tcgattgctg ccattgcgga ttggatcgag ttgatcacat
    11221 aagaagcggt agaaattggt tcagaaatct gattgatgta ctgaagataa gcttgtaaga
    11281 agccgatcgt cacaccacct gatagaacaa gcatagcacc gaataccgca cttatgatga
    11341 aggcgagttg attaatgaaa cgaatggccg gatagatagc acagttcata aattgagcac
    11401 gtttaaaagc gttgtattgc tttttattta cttcgtgtat agcagcttct gcatgagctt
    11461 gctgattgaa tgttttgacc actagattac cagctaagat ttcttccatt cgattattta
    11521 aaacacccag ttcatactga ttctgttcag aaatctgttt gtttttgttc gcaatcttag
    11581 ttgtaacaat tgaactgcca acgatcagca ataagacgag caaagtaagc ttgctttcga
    11641 tataaaacag catcagacca gcagcagcta ttgttacgac agaagtaaaa aattgattga
    11701 tacctgtcaa taacacttga gaaagttgat tgagaccggt agttgtccga ctaatgatgt
    11761 cacctacttg atgattgtca aaaaacgaca tcggtaatgt tttgaatttt tttgtgattt
    11821 cttttcgcac cctcaatgtg atcttttcgc ttaacgctgc cattgtccgt tcctgaataa
    11881 atgaagtgat gctgcttaag actgaacagc cgatcagcaa taagacaggg aacaacaatg
    11941 cttgtttgat atcactgaaa gaaagctgtc caatacctgt ttttcttatt tctgataaga
    12001 gatcatcgat cccgattccc atgatcaagg gcatggctac gactaatagg ttcccgaata
    12061 aactgcatag aaacaatccg taaaaatatt tatgttcagg tttgatcatt ttccaaaaac
    12121 gtatcaaagc acgcgtgttc aacgtttttt tcttttccat cgtatccctt ctttctatat
    12181 tccttgtgat tttgcaaact cctgataagt ttggcaatta gacagtagtt cttcatgagt
    12241 accttgtcca acgatttttc cttcatctaa aacaatgatg ttatctgctt gttggattgt
    12301 actgaggcgt tgagcaacaa tcacgagagt tttctccgac atgtgtttat gaagggctgc
    12361 tcttaatgct gcatctgttt tatagtctaa agcggagaaa ctatcatcga atacatagat
    12421 ctctgctggt ttgatcaacg ctcgagcaat acatatccgc tgtttctggc cccctgaata
    12481 attagaaccg ccttgagcaa caaagctgtc caatccgtca ggaagggtgt ccactagttc
    12541 ttctaattga gcgatcttga tcgctttaga caactcttct gttgaggcat attcatttcc
    12601 catctgcaag ttgcttaaaa ttgtcccgct gaataagaaa gccttctgag ggacataact
    12661 gatgtgggag cggatctgtt cttgagtcat ttgagtaaga ggaataccac caagggttac
    12721 ttttcctatc gtagggtcac taagtctcaa gagaagtttc gccaaactac tttttccaga
    12781 tccggtcccg ccaacgatcg ctgttgtttt tcctttaggt attgtgaaat caatatcttc
    12841 tagaactgga tcagagattt cagtatattt aaatgtgaca tgttcaaaag cagctgcatt
    12901 tgtctgatca aaggttggtt cagctactgt tttgatcgtc tcttcctcaa tttcgatctc
    12961 tgcttgcagt atttcttcta tccgtcgtaa tgaggcaaac gatttaggga taatgaccaa
    13021 taccatagct gcgatcatca agtaagcaag tgtcagcata gagtattcga ctacagcagt
    13081 cacgacaccg atttgtaatg ctccttctgc tacaagattt ccgccaaacc acaaaataga
    13141 agcataaacg attcccatga gcaaaaaagc aattggagtg acccaagcaa atattcggtt
    13201 cactttgatc atttgattag catagtcagc aaagctctta ttcgttcgct tttcttcata
    13261 tgattgatta tcaaaggcac ggatcatatt aatcccagta aagaattctc tcaatgtgat
    13321 ggtgatccga tccatttttg gctggatact caaagataaa ggtgcccctc gtttcatcag
    13381 tcgataaaca atccatacat aaaaaacaac agagatcaat gtaacccatg ttagtaatag
    13441 agaataacga aacatcatga gaatggtgaa aacgcaaata accggtgctg gtaagatcat
    13501 ttggcaaaat aaaacaatca tttgctggac attatcgaca tcattcgtca ttcttgtcaa
    13561 aagagatcca gaactaaaac gactgatatc cttcattgac agctgttgga atttacggaa
    13621 atatttctcc cgcgtttgga atccaaacct agcggaagcc aatgctgaaa agtaactaga
    13681 ggcaactgca gcgattgttc ctagaaacgc tacaagcagc atttcaactc caattttttt
    13741 tataactgag gcttcgccac ttgctatacc tgtgtcgatc agctttgcta ctaattttgg
    13801 tacacctaat gtaccggcga tttctaaaca gatcatcaca aaggtaagga gagcgagtcc
    13861 tttgtttttc tgtaagaatg tttttaagat agacatatta ttcctccttt tttcctgata
    13921 cttttcgatt ctataccttc aagtaacttg aaggtcaata gaaaaaagaa taaactgaaa
    13981 aagaggtgag aaaaaatgaa aaaaatgtat acgattggcc aagtatcgga actatttgat
    14041 ttgccaaaat caacgatccg ctattgggat gaacaaggat taatccgttc aagtagacaa
    14101 gaagagaatg attatcgatt gtttgatatc gatgatattt ttatgcttta tgatatcgtt
    14161 ttttatagaa aattagatat accgatcaaa cagatgaaga atttatatgg aaaaacattg
    14221 acggagttat atgagacact tgatgaaaca gaaagaagaa tccataaaga actggtggtg
    14281 atgaaacaaa aacaaaaaga gataagggag caaaaaaaac agttgaaatt gatgatcgat
    14341 acaaacgagg aagaatttcc tgtagaagag atcccttttg actgtatgat ctcaacggag
    14401 tttgaagata ttgtggaaat caaaaaattt ctacctaact attcaagctt tgggatgatg
    14461 agtatgccag cttccagttc acagacgatg tatggatttt ttatcgaccc tagtgaagta
    14521 catttattta ctcaagacgt gatttgggag aaaaaagata cggctgttta tcgtcgattc
    14581 ttattgaaat ccgagatgaa ccatgcggaa cgtaataata ttttagaaat cagagaaaga
    14641 atgtatgaaa aaggatggaa aacaggagaa gtgatcggcc agtatctatt gacgaatacg
    14701 gatgaaaaca atatcagaac tgaatattat catgcatgga tagaaatgaa aaaataggaa
    14761 atatgtgttt aagataactc gaaaatgatg gttgttttag ttataaagaa aaattttctg
    14821 gttcgattac gggtttaatg tgggggatta ttagtcgtgg atagatagaa ttttagtttt
    14881 ttagaggaaa tatgtcttta aaaatggaat ggttgcaatt cttagcgata tatacgatgt
    14941 aattgtcctc ttaaaaaagt atggaaaaat aattgtattg ggtgttatgt ataattcatg
    15001 gtgaactgag actaatgaga agaaaaattc atattgttat gagtgagatt gtatcagatg
    15061 agaatgataa attaaatagg tgcataaaag cattacactt aaaatacatt gttgcttaca
    15121 ttattacaat atgttaataa ggcaaaaata aatagatgga ttgaataaac atatttttta
    15181 atcaaatagt ccctactgtg actttcctca gctatcggtg atactaccga tagcattttt
    15241 ttgcttataa aataattata agtatttaga gatttttctt ctaacattct tcaaacaaga
    15301 aaaatcacaa aggttattgt tatttttata tattcttttt aagaatgatt gtttttttat
    15361 agaaatttta aaaaataaaa cacgttattg atggatagtt agtttgttat ttggtaatat
    15421 tatagaagtt gactggtagt gatttatttt tcgtcgtttt ataaaaatgt tattttttgg
    15481 aaaaattcac aaatatgtaa cgaatgtttt tctggaattt gctgtgttaa aaagcagtat
    15541 gactaatata aatactattg tcatttatgc aattttttat ggatagtaat tttagagggg
    15601 aatcaatgaa ggaagaatag aagggaaaaa tagttgcaaa tacgccagtt agcaaaaata
    15661 gcaatacatc ttcaagcttt aaaattcgag agaatcatct tggtagtgga atcaactaca
    15721 acaaaaggat taaaccaatt aatagataga attaccaatg gaaaaataga aaaggtagtt
    15781 attctataca aaagttgttt aattcgcttc ggttatgaac ttagtgaaac cctttgcaat
    15841 atatggtaca gaatatagaa attattgaca ttacagaaaa tgatgaagat ttaatacaga
    15901 ttgtcactat ttgtagatac cttttacaag gaaaccgagc caataaagct aagaagatga
    15961 ttaaggagtt gatggaagac gatacaagtt aagaaagtga aactacgccc aaataaagag
    16021 caaaaagagt aattggacgt ggaaaatgca agaaatgaac catcgattcg gtggacagtt
    16081 tattagtaat gacggtttga gaacatccat gacgagaatg aaggaacatc ctaaatgcgt
    16141 ttagttgaac caagtgccaa ataatatggc caaataagcg atcagttatc gatgaatccc
    16201 aagtattcta aactatatat tcattttgat gctaagcatt gatatatttt tttgggattg
    16261 atgaaggtta accaatacag ggactcactg atgaaattct agagtctgac cttggtatca
    16321 acgatttagt gattgtttct aatggaaaag tataaaagta tataagtaca ttaacaaaat
    16381 aactattagg gttacgctcc agcttgtgca aggcactggc aggaggaatc gtgaaatcat
    16441 aattatggtt aagccacacg gacaactaaa agtcatacaa agctatcgaa tcaatcaaca
    16501 gaagacaatc ggcaatcatt agctttgttt acagcttaca ttgcaacgct tgagttgaac
    16561 cgaaaatacg agggaggtaa cgcagccatt ttgagttggg gaatcttttt agaaaattat
    16621 cgggtacctt gttgtttagt caaaattata agggggagta atagtgagga acaaatttca
    16681 taaactgtta agtatgtttc tagttatgct tattgtgatc aatcaatttg ggaacataac
    16741 ggttctagct gagtctttag cagaacagcc agagcgttcg ctgacgatcg aagttgatca
    16801 agaggaggct gctttgggtg agcagttcca gttgcgagtc agaggaacac cagacctgct
    16861 aaatgcattg gtcattgata agagtgagga tttgattgaa ggggaagtga agcaactcaa
    16921 tgattacgaa cgtctgatta cttattcttc ccagcattca ggtaaatacg aattgaccgc
    16981 aagaagcggt gatattcaat caaatacggt ctcaatagaa atcagtgtac cagaagaaag
    17041 cacaagtgag tcttttaatg taccagaaga aagcacaagt gaatctttta atataccaga
    17101 agaaagctta agtgaatctt ttaatgaagc cagatcatct gagccatcta taacgcagga
    17161 atctcagatt gaaagacaag aggagctctt gaattatgac atcggcatct ctgctgagcg
    17221 ccccactttg ccgagcaggc atacacaaac gttaaaggga tacattcaag gaggcgctgg
    17281 cgatattctt gaagaatatg gggagatggt cgtacgagtt cctagagcat cgattggctt
    17341 aattgagttc attagagtgg aggtcgctga tgattcacct ttaaagccac tggcatttta
    17401 ccaagaaggc acagacgttg tttttccatt tacctttgat tatgaaggat taaatgaagc
    17461 cgctcagtcg tatacattca gtatcacgtt tgaagttgct ttctttcaat caggcggtac
    17521 tgagccaaat aatgactttc aagtgagtct tacttatgct gagcgagcag caaacacgga
    17581 attttcaatt acgcctattc cttcatcaag acccattttt tcgaaattat actatggctc
    17641 tagagaaatc gacagccaag gaaatcttgg gaatctgctg attgacgggc aagacgtgac
    17701 caacagtaat aaatttattt tgcaggtaaa ttatgaagga tcacgaaaag acggtgtagt
    17761 ggttaccgat gtactaccgc aaggaacaac tttggcaccg aatccgacta catggagttc
    17821 cactggaaat agagaaccta tttcaggtgg ggttcggatc gcagaagtaa cgaatccagg
    17881 tgaagcaaac gttagaactc attatgtttc cgaggaattt gtggacaaag tatcttttaa
    17941 taaggaaacg aatacacttg ttgtggactt tggtaatcta gcagagggtc aatcgtattt
    18001 tattgaatat gctcttgaag tgaccgatgt cgacttgttc aatgaaggaa gacaagagga
    18061 tgggttttct cggaatcgag cgagtatgac ctacaataat caggtaatga gccgagacta
    18121 ccgcttgaga atccctgaaa gtaatcttcc ggcaatctct ttaagaaaaa gtgtggacaa
    18181 aaatgttatc aatctcaatg aaaatgaact aacgtatact ctgcatgtct cgatgttttc
    18241 tggaacaaga aatacgcttg ttattgaaga tgttttggaa gaaggcatgg aatttgtgtc
    18301 atttctatca gatattccct ctttcattga tgccgaagtc aataatggaa tagttcgttt
    18361 cagtgcgaat aatacgattg gtggtccgac aagttttacc ctctctttta aggtcaatgt
    18421 agaggcgtat gcggtcggcg atcaaatttt aaactttgca acgcttgtga atagtgacaa
    18481 tcgattgaat acgagtactg taagaacaag gaaaatcgat gggcgtatta aagtaattaa
    18541 aactgatgag aatggccaaa ggctggcagg agcaaaattt gaaattttat caagcgattc
    18601 acaagtgata caagaaggta taacggcaga aaacggagaa tttttaagtg aaccgcttcc
    18661 tgtaggtagc tatcaagtcc gagaaacaga agcaccggaa gggtatcttt tagatgcgac
    18721 ggtacatgat gtttttataa atgaatactc catagaagaa gtgacgttaa ccattgaaaa
    18781 catccttgat acgggtggtg ttgaactaat taaacatgct gaaggcagtg gtgaagtcct
    18841 tcaaggagct gtttttgagt tacagaaccg agaaggagaa acgctacaaa ccggtttaac
    18901 tacaggagaa gatggaaaac tggcaattga cggtttagca ccaggagcat atcagctggt
    18961 agaaactcaa gcgccaatag ggtatgaatt agatgcgaca ccaatcgagt ttgagatcga
    19021 aagaagtcag acagcggtgg tagaattgac gaaagcaaac cgacttacac caggcggtgt
    19081 cgtgttgaca aagatcgatg accaaagcgg ggaagtcctg caaggagctg tttttgagtt
    19141 acaagatgcc aatggaaccg ttctgcagtc aggtctaacc acaggagcag atggaaaact
    19201 ggcgattgac ggcttagcac caggagtgta tcagctggta gaaacccaag cgccaacggg
    19261 gtatgaattg gatgcgacac caatcgagtt tgagatcgaa agaagtcaga cagcggtggt
    19321 agaattgacg aaagaaaatc gacttacacc aggcggtgtc gtgttgacaa agatcgatga
    19381 ccaaagcgga gaagtcctgc aaggagctgt ttttgagtta caagatgcca atggaaccgt
    19441 tctgcagtca ggtctaacca caggagcaga tggaaaactg gcgatagatg gcttagcacc
    19501 aggagcatat cagctggtag aaactcaagc gccaacgggg tatgaattgg atgcgacacc
    19561 agtaactttc aagatcgaaa aagaacaaga agtggcgatc tttttaacaa aagagaatag
    19621 aaaagttgct gatagctcag atataagagg aaacaatcca acaagcagtg gaaataagca
    19681 tcttccaaaa actggagaaa ccatcattag ccagtttata ttgtcaatat tgggtgtgct
    19741 acttgttttc atcagtataa aatttagaaa gaaacgaaat atatgagcta aaataaaaaa
    19801 ttgaataagc atagttttta aaatcaacta gtacccatca attgaatgga aactaccgat
    19861 tttagaaggg gtatacaaac aaatattagt atcggagtag agaaggttga acccaaccag
    19921 taaataagta aagacaattt tgtgctgcgt tgagaagtga aaaataaacc tttgtagtaa
    19981 tactagttaa taaaaaatat acaaaaacac tgcgaaaatc attcttgagt ttcgcagtgt
    20041 tttttgccta atgactcaag tttattgggt aatactttga ttttctttct acaaataatc
    20101 agcgatcagt tgtaattttt tctctagttt taatggctct ttgtcaataa ggagaaaaac
    20161 aagtcgggtc ttttgtctcg gaaagcttca accaactcgt gaataaaggt tacgcctcct
    20221 tgaaaggcgt ggaaaaacca agtaatcgat caatcaccaa tttcgcattt ggtagaacat
    20281 gtttggtgag ctgaaagtag gtggcgttca tgtctgtaac taagaattct atttctttag
    20341 gattagtgca gctaaggaaa tagtttgtta atcgaggaat tttttgtgta ggtaaatttt
    20401 ctcgattaat ttttctgtct cgccatccgc ggaaataaaa ctcagtttat cctcaacgga
    20461 aacgtgagaa cgaaattcac aaccctcaac actcaagaca gaattctttt ggaagacttt
    20521 ggtaaatagc ttctcaactc tttcaatgtg cgaataacgg ttgtcaaaga tacctaacaa
    20581 cttttcgaaa taaaaaataa cgacactttt tcagtcagta aagaagtgat tttatgtctg
    20641 acatgatttg aaatcgagtg ccttggacgg ataaaataac tttgagcgtt ccaatggctt
    20701 cgacagtttt tacaggtata gcgctgcttt tttaggcgta taaccattgg catatgatta
    20761 tattattcaa agtggacaat ggtttccttc ttcttgtttt tcaccaccat taatttccta
    20821 tttccatcta ccacagcaga accacaattt ctacagacac atggagcagg gaagagaact
    20881 gcagcaacga tcaatgtttt tttcttctgt aaggtttcgt aagagacctc tgtaatcatc
    20941 aaatttttgt ctattgatct cagaattttt ttgatagaac cattcataaa gcatatcgtt
    21001 ctctcagttg tttattttgt ggtgatttaa tcatacaaga aaacgatatg tttttttata
    21061 aaaacatgaa taggaaaaat agttgacaag aaaatgacaa cgccatatga tgttccatag
    21121 tatttacaac ctgtcataga tgactgtaaa aaataaaagt aaaaagaaag gacgacaaca
    21181 ggtggatttt tatcagtctc tacaactaga tccgtttatt ttaaaacaaa aaatacgaga
    21241 agcagcttct aaaaaagaaa agtgcatgta tatttgttca ttgtttttac gatcattatt
    21301 catcgtattg tttgccattt gttttatcat tataattaca acattatttg aatcgaagca
    21361 taaaccatac gctgtggttt tattctgtat gttgatgagt attcgttttg ttgactttgg
    21421 ttataagatt tcccattcga tcataggttt agcaatcgtc atgttctcac tattagtagc
    21481 accgtatgtc cagctgatca aatggtcagt tatgggcatg ttgattcatt ttattttact
    21541 aagcagcata ttaatggcaa ccgctagtga tcccaaaatg gggaatgctt ctttgtacgg
    21601 cttttcatat ctctttattg tttattcgtt acctaaagat tcgttgaaca agaatttctt
    21661 tacacaaaca agtagcttat tatttttgtt tttttgttgg ttttcagttc tcctttacag
    21721 aaaacatcga gaaaagaaca aagacaaaag cttgtttaaa gagatttttt tgaaaggcat
    21781 gtattcgcag gaaaaaattt ggatgctcat ctatgcattt ggcattagtc ttttgatcgt
    21841 tgcaggagag tatgtccctt ttcagcgatt gatgtgggca gggtttgcat tttcttctat
    21901 tgtatctagc tatggattga tgtcgattgg tttcaaagaa cgagcagtag accgaatcat
    21961 tggctcattg attggatgtg ttttatttat tggtatcagc caatttattc cttttaactg
    22021 ggtcggtata ttaggtggtc tagctttagg tgtttgttct acctatagat ataaaacagt
    22081 ttttaattct tttggtgctt tggcaatcac tgccagttta ttcggggttc caggagcagt
    22141 aacgatccgc atttttgaga atatccttgg ggtatgttta ggaatcatgt atattggggt
    22201 gactgaaata cttatccgaa aaataagaga aaaacacggt ttgaatcatt agatgagatc
    22261 gaataaaaaa taaggaaaaa gtgaagatac acatggatga taaattgcta tcattccatg
    22321 tgtatctttt tacggtaaat cagtgaaacg ttcgactttc atttttaatt tatacttttt
    22381 acggagttcg tttatttgag ccatcatttc ggatttgtga tgcttttcga ttgcttcttc
    22441 atctttccaa cggtcgatca gtaaaagaga ttctggatct tctagtggaa agtaatagtc
    22501 atatttctcg tttccttctt ctgcacgaat cgctttgaca atgtttcttt cgaccatttc
    22561 ttctgcaaat tttctggctt gtccatttgt acttgtatac aaaatattca ctgtgatact
    22621 cactaaatcc cctcctgaaa aaattctatc actagtatat cacggtgcca gaaattatta
    22681 ttcaagatat gatacacatt ttttccgcaa tgctacggaa atatatattt tgaatagaaa
    22741 acgcttacaa gatatgcttg gtttataaaa ataaaaggag tgattgattt atgaaaaaag
    22801 cattgattat ttgtgcggca ggaatgtcat cctcgatgat ggcctcaaag acaacggatt
    22861 tctttaagca aaaaagagaa gaggtactcg tagatgcagt ttcagctaca gaaggcgata
    22921 atatgattaa gaatagtgat tttgatttat ttctgatcag cccgcaaacg acgatgtttt
    22981 tggataaatt caaaaaatta ggagaatctg ttggaaagcc tgttgttagt atccctttcc
    23041 aagcgtatgt cccaatccct acaggcatcc agaagttagc agaggtgatc gaagagaaca
    23101 tatgatggaa taggagtgga tgataatgaa acagaaggaa aaattgctgt tgagatattt
    23161 gattgatcac aagaaggagt atgtcaccag tcaacgttta gcttctgaat tgttattatc
    23221 tgatcggaca atccgtaatt acttgcagcg gatcaaagag ttagtagaaa aaaacggagg
    23281 taagatcatt gctaaaccag gttatggcta ccaactgcat attttacaaa gactgacatt
    23341 tgacttgttt ttgagtcggc aagaaatagt acctggatat gctagagaac cgcaaggatt
    23401 ttatgagtcc gaagatcgac aaaagtatat attgaaccaa ttgcttttgg aagatgccat
    23461 tctttttatg gatgatttgg cagaagagct ttatatcagt cgttccagtt tgacaaagga
    23521 catgcaagaa atcaaggagc gattgattcc atattcacta aaaatcgttt ctaagcatgg
    23581 tcaagggaca tggatcgaag gggaagaaag aaatcgacgg cattttatca tggatacttt
    23641 ttttggaaac cattatggaa attcattgaa agaatattta gggcaaagcc gttttttccc
    23701 agacatcagt tttgaagagc tagtcattat cattctcgat gaaacaagag aagctaagtt
    23761 aaaagtttcg gattttatca tacagaatct gactttacac ttggctttgg ggatcaaacg
    23821 gctccgagaa gggtttgaaa tcaaggaatt aggcatcgaa caagaagttt ctaagcgagt
    23881 agagtatcaa gtggcgcagc ggatcgttca acgtattgag gtaatcgcag gtgtatcgtt
    23941 tcccgaagaa gaaattgctt atttgacatt acatctcatg gctaagtcga atcacaaaga
    24001 aaatcaaaat gatcaggaac tatcaaagga attagcgaaa gttctgaaaa gattgtctca
    24061 ggaatttcat ttttcattag tagaggatct ccagcttttc aatggactac tcgatcattt
    24121 gtcaccgatg atgattcgat tgagccgagg tattcgcatt gagaacccat tgactgagga
    24181 gataaagaaa gaaaatccca aagtctttga cgcagtaaaa aggcatttca gcaacatgcc
    24241 ggctttgaaa aactatacca tcaatgaaga tgaatgggcc tatttggctt tgcatctcat
    24301 ggcggcattg gaaaaagaaa gagctgcgca taaactacat gcattgatta tctgtgcaac
    24361 gggttacggc agtgcccaac tattaaagaa tcgcgtagtc agtgaattcg gtaaaaatat
    24421 tacagtggta agtgtaaaag gctattatga gatcaatgaa acaactttaa aaggaatcga
    24481 cctgattatt tcttctattg atctttcgac aatgtttttc aaaattccag ttttacatgt
    24541 cagtattttt ttgaatgaag atgatgttcg taaaatacga aaggtagttg gagaatctat
    24601 accctatcat tcctcagata gaccgatacc agcattttct ctacagcaaa aaaagcagat
    24661 ttacaccgaa cagttgtctg aaaatttttc gagacttata cgtacgaacc gaaaaaggaa
    24721 gaaattcttc atgatttgct gcaaaagctg agcataaatg aagaagaagg atatataaga
    24781 gaaatgcgaa agcagatcca gcaacgtgaa aatatgggac aaatcatatt cagtgataca
    24841 gtggcagtcc cacatcccgc tgttccagtt ggaatcgaaa caaaggtagc cgttgcattg
    24901 attccttctg ggatgcagtg ggatcaatat caagaaattc attttgtttt tttagtttcg
    24961 ccttcttatg tcgaaaatga aggaattacg gtcgtgacta aagcaatcgt taggttagtt
    25021 gaccagctaa aagtacagca ggaaatctta gctgaaccta tttttgagaa tttcagcagt
    25081 aaatttatca aactgatcga ataaggagga aaacagatgg ctgaaaaaat aaacagtgaa
    25141 acactacaag tgacttcttt tgatatcatt ttccatagcg gaaatgcacg gacactgata
    25201 catgaagcat ttcgctcgat gcgtaaagag atgtttgaag aagtagaaga gaagttgcga
    25261 caagcaaatg aagagattct tcatgcgcat cagtcacaaa ctcaacttct gaaagaatat
    25321 gccagcggga caaaaataga aatggaaatt atcatggtcc atgcacaaga tcatctgatg
    25381 acgacgatga cattattgga agttgcacag gaaatgaatt atttgtatca aaaaaataaa
    25441 tagaaaggaa ggatcattgt gcaggataca gtaaaaagga aaatcgaatt agaaaaaaag
    25501 caacttagtt tgaaaaacat gtatttcaat cgttatctaa tcgtacgtta cctgactgcc
    25561 tttttctttt tcatgaacat gcaatggatg attctgcttc tatcagctaa aagtttagga
    25621 agtagtttac cgatgattct gattttagct attttgcctg cagtaggaga acaagtaaag
    25681 ctttatcgaa aacaccaaac gaacgttcca tggacaaagt ggtatttttt gttccagagt
    25741 gtctttaatt tattagtgat tccagtgttg tttacctcag gatttacatt cttgtatcct
    25801 tttatggcaa ataacaatgg aggacagtta tttgttttca ttcttgttgt aagcggtatt
    25861 tttgccagtg catttataca gtatcgttta aagaaaattt cactcaacca agaccaacag
    25921 tatatccgta ttaaacaata tgaaaaagcg ttatatttag gaaaggagaa taactaatgt
    25981 ttgatttttt gcagaaatac ctgatgggac ctatggggaa aattgcgcaa cttaaaatcg
    26041 ttcgggctgt catggcagcg gggatggctt ctattccttt cacgattgta ggctcgatgt
    26101 ttttggtttt aaatattttg ccattaccgt tcccattttt agaaggattt tttaatgcaa
    26161 cttttttcaa agttagtgac ttgtacatga tcgtcaatac aatgacgatg gggattttat
    26221 ctatttactt tgccattgtg tttgcttatg aattaacgtc aattgaacga gatgaacaaa
    26281 aattaaatgt taatccgctc acgggggcat tactatctgt ttttgcattc tttatgtgta
    26341 tccctgaatt gatcatgact gatggtaaaa tcagcttgat tgcttctatc aatgagacag
    26401 aaactgtagt aaatggtatt aggatgggct cttttgtcga acgtttagga acttcaggaa
    26461 tctttacagc tattattatg tcgattattg cggtcgaatt gtattgtatg tgtgtcaaga
    26521 gaaattgggt cataaaaatg ccagatgttg ttccgccagg agtttctcgt tctttcactg
    26581 ctctgatccc aacatttgtc attgcgtttg tggtgatgat catcaatgga ttattagttg
    26641 cactaggaac ggatatcttc aaaatgattg ctatcccatt tgggtttgtt acgcagttga
    26701 cgaatacatg gcttggtgtc atggtgattt atttcttgat ccatgcattg tggatcgtag
    26761 ggatccatgg agcgaatatc atcacttctt tcttgacccc gatcgtatta gctaatatgg
    26821 cagctaacgc acaaggcgca aattatccgt tggcaggaga attcaacaat tcttatgtaa
    26881 ccgttggtgg atcaggtgcg actttaggat tgatcatctt tattgcattc ctcgcgaaat
    26941 ctgatcagct gaaagtgtta ggaaaagcct cacttgtacc aggattattc aatatcaatg
    27001 aaccaatcat tttcgggatg ccaatcgtct acaatcccta tttagcggtt ccgtttttct
    27061 tagctccgat ggcttctgct acactcgctt attttgccat tcggctggaa atcgtcaaac
    27121 cgatgcttgc acagatgccg tggccttcac cagtcgggat cggagcattt gttggtagtg
    27181 gtggtgattg gaaagctgct gtgttagcag tcatttgtgc aatcatcgca ttcattatct
    27241 ggttcccatt tatcaaattc tatgataata aattattaaa tgaagaaaga gaaaaggcag
    27301 cagaattagc tagtgaaaag ttaaaatgat ttctaagaaa aaaggaactg tgacaaaaaa
    27361 tcacagtccc tttttttctt agaaatggga aaccactagt atataaatac cattattctt
    27421 caatcaaccg cttcacgatt gcataatccc cttcataagg ttcatctaca ccattgattt
    27481 tttgataggg atccacgcct tttcccgcaa taacgactga atcttgcgga gcgctttctg
    27541 ctaaagcgcg tcgaatcgct tcaggacgat ccatttcata atgaacgatc aaatctggat
    27601 tagtaattgc ttcattgatt tcttccgcga tttttttcgg atcttcaaat gctgggtcat
    27661 ctgccgtcaa aaaggcgatg tcagctgttt ctgatagcac ttttccaaaa tcatgtcgac
    27721 gggagattgc tttgtttcct gggctgccta atacaacaat tacacggcca tctggatgtt
    27781 catttttggc aaatttcagc aatgttttca agcttagata attatgtgca tagtccacat
    27841 agacatgcgc tccattggat tggatcaatt gatccattcg gcctggtaca cgagcttctt
    27901 ttaatccgtc ttgtgcatct tgactggagg cacctgttag tgctgcggca atgatcgcac
    27961 tggctgcatt ttcttggttg aatcctccag ccagcaaaat atcataagca gtatcgcaga
    28021 tagctaagcg atcttcatga gaagataaag taaaaccgtg agtaccttta tctgaacgga
    28081 tcacttgata attgctttct tccgaacgac cataagtgat cgttggaatc tggaatagat
    28141 cggctgtttc cctaagtaaa tcaaaatagt cactttcatg atttaaaaca actgtttttg
    28201 aattcaagat caattgtcgc ttgcaataga aatagtcatc aaatgtcgga tgttcgatcg
    28261 gactgatatg gtcaggcgag atattcaaga agattccgac atctaaagtc aagccgtata
    28321 cgcgttgtgt tttgtaagct tgtgaagaaa cttccatcac tagatgagtc ataccatttt
    28381 caactgcttc tgacatcata cgatataaat ctaatgactc aggcgttgtc aaatgggatt
    28441 tgaaatatgt tttgccatct aacgttgtat tcatagtaga aagcaatgct gtttttttat
    28501 ttgttgtgtg atccaagatt gcttttgtaa agtacgcagc agttgtttta ccttttgttc
    28561 cagtgaatcc gatcactttc aatttatttt gcggatagtc gtaaaaagcc atagcaagaa
    28621 gcgccattgc tttacggata tccgttacga tgattcctac tgctgaattt tcgtatggtt
    28681 gttctgatac ataatattga ataccctgtt cgattgctga atccaaatat tcttctttga
    28741 aattcattcc tttacagaaa aacaaagtag aagcatcagc ttttcttgaa tcataactta
    28801 actgaagaaa ttcagctttt tctggtacat caagatgcca gcctcttgtt gaaacaaatt
    28861 cttttaatag attttctttt aatagtaaat gacggatgtc ttctaaagag aagctcattg
    28921 attgatccct cgctttattc aatttcttgg tttattatag cacactcttt ttaatatatc
    28981 gtttgtttcc gctatgacaa aatccagctt tattcaaatt ttcttgacac aggggacaag
    29041 cggatgagta ttttcataaa caatgaactg actgtaatag ataggagaac gaatagtgtc
    29101 tccaacaatc caaaaacata ataattcaaa aagacgataa gactattggt aagtaaaagt
    29161 ccttgcggca ttggaagccg aaaaaatttg tggaaaataa tcggcggtat cgttataccg
    29221 ccatttgagg ccccgactgt gtacaatgtg taaaccccaa gactgaacaa aagacttcct
    29281 gaaatcagtg aaaagaaatg tgaggagatc atttccactt ttggaacaat ctctaaaaca
    29341 acaggaaaaa gcagacttcc cgctacagtc ttaaaaaata ctgattttcc taataggaca
    29401 gcggctagaa gcagcatcat aagattgatg ctccaaatag taactgagcg ttggatagaa
    29461 aaggccgttt caactaaatg tccgatggcg cccactccgg cggaagctat atcgtgaggg
    29521 agcaagaacc aattcaatcc aactgtgata aagaaaagac ctaaaaatat aacgggtagt
    29581 ttttttattt cttgtgtcat tgatatctcc tataaatgtt agccataaaa ggctgtatat
    29641 ttctttactt taccaaaaaa acgaaaccga attcaaaaaa acaattgttc ttataaaagt
    29701 tatataaaat cgattgccag tttctgtttt accaattaaa atgatagtaa tgttttgtag
    29761 aaagttggaa tctgaccgat ggataatcaa agaaaaccag cattagatca attaacaccg
    29821 caagaaaaaa tggcacgagg ctctgcttgg atgaccgcca gtaatatcat ttcaagattg
    29881 ctaggcgctg tctatatcat tccctggtat gcttggatgg gcgaaaatgc taaggctgcc
    29941 aatggattat tcaatatggg atacaatata tatgcattgt ttctactgat ctctacagca
    30001 ggaatacctg ctgcgattgc aaaacaaact gcacgctata attcattgaa tgaatatggc
    30061 acttcacgac gattatttat ccgtgcatta cagatgatgg gcgggttagg cctgttgttt
    30121 gctttgttca tgtacatcgc ctctccttgg ctagctcatg cttctggtgg cggagaagaa
    30181 ctgattccaa cgatgcgttc tttgagtatc gctgtattgg tttttccttg tatgagtgtg
    30241 atacgagggt atttgcaagg caatcaagaa atgatgcctt atgcgctgtc gcagattgta
    30301 gaacaggtgg cacgtatttt ttatatgcta ttggctacat tcatcatcat gaaagtcatg
    30361 gatggtgact atgtgacggc ggtcacgcaa tcaactttcg cggcatttat cggtatgcta
    30421 gcaagtattc ttgtcctttt gtattttttg aagaaacatc aagcgtatac atcggcgttt
    30481 attcactaca gcgaaaacaa aataaatatt gcaacgaagg aattgttgtt agataccgtt
    30541 aaagaagcga ttccgtttat tattgtgggt tcaggggtaa cgatcttcaa attagtcgat
    30601 cagttcactt ttatgaagat catgtctgca tccaccgagt attccaatgc acagttactg
    30661 gatctttttt cgatttttag tgccaatcca gataagttga cgatggtagt tattgcattg
    30721 gcaacatcga tcgcgtctac tgggttaccg ttgattacag aagctgttac gttgaaagat
    30781 cgagtaggtc tggcaaaatt gacaagcgga aatttacagt tgttttcatt ttttatgttt
    30841 ccggcgacat ttggtgttat gttacttgcc tatccgctaa atacattatt ttatacaccg
    30901 gattctctag gaagtaatgt actgatccaa gcaagttttg taggactctt cctaggacta
    30961 tatatgctag tttccaacat gctgcaagga atgtttgaaa acaaggcggc aattaattat
    31021 ttggttattg ggtttctagt gaaactcctt cttcaatacc cagccattcg tttatttgaa
    31081 gtatacggcc ctttattggc gacaatgatc ggatttgctg tgtcttgtac cttgatctta
    31141 aagagaatcc atgaagtagc ccgctttaaa ccaagttttg tctggcgcag gacattgctg
    31201 atctttattc ttaccttgat tatgcttttg gcttcgtgga tcacaaagat gatttttggt
    31261 gcttttttaa acgaagatag caaattccag tctctattgt tgattcttgt agtagcaggt
    31321 ataggggctt tagtgtatgg ttatttagct ttgaaaatcc gcttggcaga aagactacta
    31381 ggaccaggaa tagcgcgttt acgacataaa ttaaaaataa aataagtgaa tcagaagagc
    31441 tagattgatg ctcttttgaa atgggggaaa cgcatgcgtt tagataagtt tttagcagat
    31501 atgggacttg gcagccgcaa agaagtcaaa ggattaatca aaaaaggaac gatcgaagtc
    31561 aatggacaag tggtgaaaag cgataaagcg caagtaaatg aattcgaaga tgcggttact
    31621 tatttaggag aaatcattac gtatcaaaaa gatttttact acattttgca taaacctgca
    31681 ggagtcattt ctgctactca ggataatcat gatcaaaccg taatcgattt attggaagac
    31741 caggattatc gggaagatct ttttccagtc ggccgattag ataaagacac agagggcctc
    31801 ttgattttaa cgaatgacgg ggtgtttgcc catcagttac tatcacctaa aaaacatgtg
    31861 gagaaagaat atctagctga ggttagcggt ataatgacag aagaagatat ccgtttattt
    31921 gcagaaggac tgattattga tggcgatgag cagacattgc cggcaaaact ccaaattgat
    31981 caagtagatc aagagacaga aacttcaatt atccgtctga ttttgcatga aggaaagttt
    32041 caccaagtta agcgaatggt caaagcagta ggaaaagaag tcttatattt aaaaagaatc
    32101 cgtatgggaa aattttggct gcctaaagat ttgaataaag ggcaatatcg acccatgacc
    32161 aaagaagagc tagaacaagt gagagaaaaa taattgaatg gatagctaaa agaataagca
    32221 gagtagttgt agcgccatct gaaaagcaga cttgtccgtc tgactttcgg atggcttttt
    32281 tgtgaaattt cggcttattt ccgtagaagc tacttctgga acaccgtttg ttcggaataa
    32341 gggactgtga caagactttt gttacagtcc ccattgttcc ttgtgactca gagttagata
    32401 gtaaggacac gatctcccta atactcaggc caaaacgctg aattcacaac ctctgataaa
    32461 cggtgttcaa tcagctgacc ctcggtatta gctcaaaaaa cggcaaaata tgaggaacta
    32521 ttttcccgtt tttttctcta atactcaggc caaaacgctg atttcacaac ctctcctata
    32581 ctttgtttca ttttccctat actttgttta tttattctca ttattgaaag cgctataatt
    32641 acgttgcgaa tgaatcgaaa ggggttttta gatgaaaaaa cgaaaggggt tcaatcattt
    32701 ttttatgaat ctatggcgtt ataaagcact tgtcctgatg gctgtaccgg gtatgctttg
    32761 gatgattttt ttcttttaca tacctgtaat ggcgaatgtc gtagcattca aaaattttca
    32821 tatttcagca gatgggttct tggctagctt gagggaaagt ccttgggtgg gttgggaaaa
    32881 tttcaaattt ttattttctt cggatcaagc atttctcatc actaaaaata caattttata
    32941 taatgtagca tttatccttt tgaatctgct gatctcagtc gtatttgcaa tcattatgag
    33001 cgagttgaga aataaacgac tcgttaaagt ttatcaaacg atgtccttac taccttattt
    33061 tctatcttgg gtcattatca gttactttgt ttacgctttt ttaagtcctg acaaaggaat
    33121 attcaatcag tggatcactg gacatggagg agaagctgtc aattggtata atgaaccgaa
    33181 atattggccg tttatcttgg tgttcattgg tacatggaaa gggattggtt ataacagcat
    33241 tatttacttt gcatctgtta tgggaatcga tccgacttat tatgaagctg ctatggttga
    33301 tggagctagc aaatggcagc agatcaaaca tgtgacgata ccacaactta ttccactaat
    33361 gacgatttta acgattttag ccgtaggaaa cattttccga gctgattttg gcctgtttta
    33421 taacattcct aggaattccg gtgcattata tgatgtgact caagttttgg atacgtatat
    33481 ctacaatggc ctgacatcca caggagattt tggtatgacg gctgctgcag gactttacca
    33541 atcagttgtc ggctttgtct tgttgatgat cacaaatacg atcgcccgac gttttgatag
    33601 cgattctgca ttattctaag gaggaaaaaa atgaaaaaaa agaaagtgga gagagtccaa
    33661 gtccgctctt tcaataagac ggctaatttt ttctctaata taatgattgc actctttgct
    33721 atttcctgta ttttgccatt tatatttgtt atcgccatct cttttaccga tgagtccata
    33781 ttgtcgactc acgggtacag tttttggcca gaaaagttca gtatatttgg ttatacgtac
    33841 ttgttcgacc agatgcagga taaaattttc caagcgcttt tcgtcactgt acttgttact
    33901 gttttaggta cactgatcaa tagcacggct acttctttat atgcttatgc gatttcacgg
    33961 tcaaactttc cattccgtcg tttttttacc gttttttgtt tgattacgat gttgttctca
    34021 ccagggatgg tggccaatta tcttgttatg acaaatcttc ttcagttaaa agatacgatt
    34081 tgggcattga tcttgccaat ggctgtcagt cccttcaata ttattgtgat gcgcacattc
    34141 tttaaacgtt cggtctctga ttcgattatt gaatcggcac ggatcgatgg agctagcgaa
    34201 ctgcgaatat ttgttcagat cgttttaccg ttagctgttc caggtattgc gacaatcagt
    34261 ttatttgccg cattgggtta ctggaatgac tggttcaatg ctttactata catccaagat
    34321 gataagctag ttccactaca gtatcttttg atgaaaatcc aaagtaatat tcaatatctg
    34381 acacaaaatt caggagcagg aagccaattg acaggcggat tagcctcagt tccaggagaa
    34441 tctgccagaa tggcgatcgt agtcatctcg acgttgccta ttgcgatcag ctatccattt
    34501 ttccagaagc attttgtcaa aggtctaaca atcggcggag taaaagaata aagaaacaaa
    34561 aatataaatc ataaaattag gaggaagaaa aatgaagtta tggaagaaac tagcctttac
    34621 aggtgtttca gcgttgttgt tgggaacatt agcagcatgc ggaggttctg gttctaaaga
    34681 ccaggcagaa gctagcaatt cggacacttt gcagatgtat caaatcggag ataaaccaga
    34741 taatttcgat caattgatgg aagtagcaaa caaacgaatc gaaaaagaga tcggtgtaaa
    34801 agtcaatatc aattacattg gctggggcga ttacgaaaag aaaatgaacg tcatcatttc
    34861 ttccggggaa aattatgata tcgcctttgc gaacaactat gtttccaatg cccaaaaagg
    34921 agcctttgct gatttgactg aattagcgcc taagtacgct gaaaaagctt ataatgattt
    34981 agatgaagca tatattaaag gaaatctggt caatggaaaa ttatatgctt tcccagtcaa
    35041 tggaaatgtc tttgctcaac aagtacttac ttttaacaaa gctttactag ataaatacga
    35101 tctttcaatt gatggaatcc aatcttacgc tgatgcagaa aaagtcttga aggaattcca
    35161 tgaaaaagaa ccaaacacgg cagcttttgc cattggacaa ggcttcaaag tccaagggaa
    35221 ctttgattat cctttaggga atacgttacc atttgctatt gatctggatg gagatgattc
    35281 gaaaatcatc aaccaatacg ataatgaaac gtatatcgga ctgttacgta caatgcatag
    35341 ctggtacaaa caaggactca ttccaagtga cgctgcaacg agcaataagg attatccact
    35401 agaaggaaat acttggctaa tgcgtggaga aacacaagga ccgtatgatt atggcgatac
    35461 aattttgaca aatgcagcaa aacaagaact tgtctcaaaa gcaattactg ttccactaaa
    35521 atctacagct caagcacaaa tggcaaattt tgtcgtatca aatacttcaa aaaataaaga
    35581 aaaatcagta gaacttctag ggctgcttaa cagtgatcca gaattattga atgggcttgt
    35641 ttgggggatc gagggagaag catgggaaaa agtcgatggt tcagatgata aaatcaaatt
    35701 gctagatggt tatcagccaa atacacatat gtctgcttgg aatacaggaa acaacaagat
    35761 tctatataca caagagtcta ttacggatga catgattgcc aaacgtgatc aatcgattgc
    35821 tgatgcagaa acttcaccga ttttaggatt cagcttcaac actgacagtg tcaaaacaga
    35881 attaagtaat atttctaatg tcatgaatca atatttagat ggattgaata cagggactgt
    35941 tgatccagat gaaacattac cgaaattgaa agacgctttg gacaaggctg gctatgataa
    36001 ggtattgaaa gaaatgcaaa aacaatatga tgagttccga caagaataat aatgaataaa
    36061 gacaaagggg ttgtgacaaa agacgtgtca caacctcttg ttccgaataa acggtgttca
    36121 aaagccagtt tctcggtatt aagctaaata tatgtaaaaa tgtggaaagc catattcaca
    36181 aaatttttct tactactcga aactaagcgg ctatttcaca aactcttggt aaacggtgtt
    36241 caatcagcta accctcggta ttcgctcaca aaacgacaaa atatgaggaa ctatttttcc
    36301 gtttgtttct ctaataatca ggtcaaggtg ctgattgccc aacctttttt tgtataatga
    36361 gaaagtataa acttaatgtt tggaggagaa gatatgttag ggaaggtttt ggaagagtta
    36421 tttatccgga tctgggtcat tataaaacta accttgtatt tctggatata tacatttgcc
    36481 ggcgggatca tctttggatt aggtgcggca tggaaaacag tcaatgaact attttatttg
    36541 tacggattcg agtataaaga aatcacaata aaaagaggct ggaatatata taaaagaaat
    36601 tttttaagag gcaatctgct atttagttta tttttatctg gtactgcttt attgagttac
    36661 aatttatatt tatctgttca aatcaaaggg ctcatttttt tagctattga ttttatttta
    36721 ctttttgcct tgctctgcct atttgctact tatcaataca gtatgatttt agatagtgaa
    36781 tatacgatct ctgttcctaa tctgctaaag ctaagtttta tttcggtttt ttccagcttt
    36841 tcgtcttttc taaaaataat tattggtagc ggtataattt taggcgtgac ttggcaattc
    36901 aaaggattga tcctgtttgg tgtcattggt cttttgacgg tttggaatgg tacgatgaca
    36961 acacattggc gtgaagaatt agataaacag ttggaaagtt atgaataagt ttcggattct
    37021 attttcaaaa ggaaccttac taaatcgtct gttgcggaaa tacgcggtta tcatgatcgg
    37081 tgtaacgatg actgctaccg ttatttttag tatccatact tgggaacaaa atcaaaaaca
    37141 ggcagaaaac atgacaagtg atgccgttca aagcacaagc agaatgctga atgataaaac
    37201 aaccttgagt aggatcatca aaaaccagct tgtagggaat tccgaaaaga tcgaaaatgt
    37261 gacgacctac ttaacaaaac ccatagatca gtatctgatg tatgtatacg aacagcaaaa
    37321 cagtaccgat gaattagttt ctttcccaaa tcaaatcaaa gatctctacg caaattatga
    37381 agaactaagt gcgatatata tcgtgttgaa tcaactaccg gagtattatg aatcttctag
    37441 agaaaacaaa ggtggggaaa taaaaaaggg gacaccaaaa ttactagacg ccttttatat
    37501 caagttgcct atcacacaag gaggagctga gtcaggaagt atttttattg gttttcaaaa
    37561 gaaagaacta gatatgattt tagaaaatct aacgtcattc aatggactct ctttatacat
    37621 gatttctgga acgacaaatc gactatacac attccatgat aataaaatag ctaaagaaac
    37681 gtttgaaaga caagaaatga tcatcacaga gagtctgaag aaaaattcac aattgccgat
    37741 tgacgatctg gaaaaaaaca attttgttca acaccaagaa ctgtctgatg attatcgtat
    37801 attagctgta ctagataaaa aaagtgtaaa aatcgaaacg ggaaaaaatc ttgccccgct
    37861 gatcattggt atcattattt tagacaatat actgcttatc tttctttata aaacattcaa
    37921 acgatattca cagcaagtat ctatcgtcat gcaggccatg gatcaaacag cagcagggaa
    37981 tttagagacg cggatcgaca taacaaacac ggaatatgaa ctgaaggaac tttcaatcgg
    38041 tatcaacgaa atgttggata gcatcaatca gtttgtagaa gacatctaca aattagaaat
    38101 caaacagcaa gacgcacata tgcgggcgct tcaagcacag atcaatccac actttttata
    38161 taatacatta gaatatatcc gaatgtatgc aatcagcgaa ggaagtgaag aattagcaga
    38221 tgttgtgtat gcgttttccg ctttgcttcg aaacaatacg aatcaagaga aaacgatcac
    38281 tttaaaagaa gaactggact tttgcgaaaa atatgtctat ttgtatcaaa tgcgttatcc
    38341 taatcgagta gcctatcatt ttatgatcga tcctgatttg gaaaagatcg aagtacctaa
    38401 atttgtgatc cagccattag ttgaaaatta ttttaaacac ggtattgatt ttactcgatt
    38461 cgataatgcg ctaagtgtca aagtcttgca ggaaggcaaa cgggtaagga tcatcatcaa
    38521 agataatgga aaagggatga ctgaaaaacg gctaaaacag attgaagaaa aactgtctca
    38581 tcctaaagtg gaacttcacg gttctatcgg tctgcaaaac gtgaatgaac gtttacgtgc
    38641 cagcttcggc agcagttatt atatgtcgct tgagaataat gagacaggtg gactgaccgt
    38701 ttctattaca ttcaaggagg gatgagcgtg tatcaagtgt tattggtaga tgatgaatac
    38761 atgattttga atgggttaaa aaagataatt gattggcaaa gtttaggctt tcaaatagta
    38821 gcaacagcag agaatgccaa agaaggacta gctgtacttg aacagcgaca gattgattta
    38881 gtagtaacgg atgtgacaat gccagaaata aatggactgg aatttattga agcggctcaa
    38941 aaagaacgtc ataattttga atttatgatt ttatcaggat accaagaatt cgattatttg
    39001 aaaggaggta tgcaattagg cgccgtgaat tatttgatga aacccgtgaa taagttcgaa
    39061 ttggttgaaa gcctcaagaa aatcaaaaca cggttagatc agcaaaatga acaaaaaaat
    39121 caacaagaaa tctatcaaga actgttattc agtcaatggt tgaatgaaga attggatgaa
    39181 ataagtgaag aagaaataat aggacaaata agtgagaagc agcataggat agtattgatt
    39241 caactggcaa gaactcatga tactcttatc aatagatggt tgcaagaacg gcaacaaatc
    39301 ttttactatc aacgaaatta cggagaccgt gtattgatca cgttgttgtt ggaggcagat
    39361 gaagttgatg gcttctgttg ttttgttcaa gaaaactttc ctgatcagga atggctgatc
    39421 agtatcgggg aagagacaga ttctttggac aaaatccctg aaacattcca acaagcgaaa
    39481 gacaatctac agttgcatca attttatgga gataaagacg agcatgtttt ttacgcagta
    39541 caagcagaaa caaaagatca aaccatcgat ttttcaagtt tcagacgcgt gctgcaaaat
    39601 aaacaattgg aagtcgctca aaaaatgatc actgattttt ttgaacagtt tcagttagca
    39661 gccatgatgc cggaagatat acgatattcc agttttttac tgtttatgga gattcaacgg
    39721 gaattgattg atctggaaga tgaggaatat ttacaaggaa tcgaaaaaat ccatcaggca
    39781 aaaacggtgc aagaacttca gcagctactt ctgtcattca tcaaaaagca tcagagacag
    39841 aaaaagtaca gtgacaatgt ggaaaaagta atcgtaatcc tgcaccagca ttatcaagaa
    39901 ccacttacac tcaaagaagt gtcagaaaac ctgcatttaa atgtcatgta tctaggacag
    39961 ctgttcaaaa aagaaacgaa aaaaagtttt tcagcttatt tgaaccattt acgtatggaa
    40021 aaagcgaaac aactgctttt gcacagtaat caaaacatca atgagatagc aagtgagatt
    40081 ggctacaaca acaccacgta tttctctaaa ttattcaaga aaatcgtcgg cagatcccct
    40141 aaagagtacc gagaaaacag tggagaatca taaaagaaaa agccaatgta atgaaataac
    40201 ttataaaaaa ttgtctagaa atctaatttt gggctgttct tattacttga ggcaaggcag
    40261 ctttttcatg acctctcgat aaacgg
//
LOCUS       DACGLE010000018        36767 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0534
            SAMEA3304449-rid9560453.denovo.019, whole genome shotgun sequence.
ACCESSION   DACGLE010000018 DACGLE010000000
VERSION     DACGLE010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304449
            Sequence Read Archive: ERR985064
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 36767)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36767)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702225.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702225.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 20:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,045
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 27 of 67
            Pseudo Genes (incomplete)         :: 25 of 67
            Pseudo Genes (internal stop)      :: 19 of 67
            Pseudo Genes (multiple problems)  :: 4 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36767
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304449-rid9560453.denovo.019"
                     /strain="VREN0534"
                     /db_xref="taxon:1352"
     gene            complement(<1..334)
                     /locus_tag="IXT20_001060"
     CDS             complement(<1..334)
                     /locus_tag="IXT20_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4859522.1"
                     /translation="MKKVTREEVASILVKDKYFSKGNYWLKIWQTLVALLGWMIVVLP
                     FIWVFFPLTRPERAKDFSLYAFLSEKKMLYFLIGFFVLIFFSFLITSFVLTRWNNRNL
                     YKKVNKSFE"
     gene            complement(509..2644)
                     /locus_tag="IXT20_001061"
     CDS             complement(509..2644)
                     /locus_tag="IXT20_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAQ4859523.1"
                     /translation="MVNTQKQLEEAYLKNVYQKLIDKKNELRESMDTAKIEGNQSLKE
                     MAGELRLNFDSYLDNLDTFSMIEMKNREIDQMNIKQKNAENQLAKIERLLKSPYFGKV
                     VVDFLDNEPKESFYIGTANFTDDADENLVYDWRSPIAELFYNNMIGPSSYTVRQNKID
                     TVIKERRQFLLERDRLVKFFDTSVAIQDDVLLEVLGQDETNEMKAITATIQSEQNTII
                     RDVKSQNILVNGVAGSGKTSAIMQRIAYLLYLYRDTMTSDDLLILSPNHRFIDYISNV
                     LPSLGERNPLNLTIIQLVSQLSAEEIEGEEAYFKRITGENVSEQTERLRSKKFIDNLK
                     QSDPLFLDHPNFIRGLTKNGKTVLSKKTIEKIYEKVPAHPKLIDRLQATKKALMSEWK
                     NHLLKQAKSPAVQNQVLSLTEDRQLELFGKLISDDSEQSIVAYARKLLQKKYRKITRQ
                     IEEMAWVDEHQLFERIYEKRYGSAYAWQPTRTVDEAVIILAIRHLLVEKVNVPAFRYL
                     LIDEVQDYTLAQLGLLIELFPKTHFTLVGDENQAIFNSSTTFADIMRCFDDYHLPIHR
                     YDLRNSYRSSGAITELFKTYAVDQEKIDIVSIRPQGKEPEYRSFRSSEELLKILAEIL
                     VSLKGEKAAVITQTEEETQTLQESIKKNSYGLENCQIIPLSLAKGLEFDHVILYPFEN
                     DGDEQRRRRQMYTAISRGMKSIVVLERAT"
     gene            complement(2727..4382)
                     /locus_tag="IXT20_001062"
     CDS             complement(2727..4382)
                     /locus_tag="IXT20_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAQ4859524.1"
                     /translation="MKIVIVGGVAGGMSAATRLRRLMEDAEIVVFEKGPYVSFANCGL
                     PYYLSGEISERENLLVQTPESLSARFCLDVRPNHEVTAIFPENKTVEVVHEGQKHIEQ
                     YDALVLSPGAKPVVPSIPGITEADNVFSIRNVPDIDKVMHALEKQPKRAVIVGAGFIG
                     LEMAENLKRRGLEVMVIEQAPHILPTLDEEMAAFIEKELSHQGVEVITSHAVAGFEDH
                     GKRLRLDDGRTIPADLVILSIGVRPDNQLAVTAGIELGIRGGILVDERYQTNIPDIYA
                     VGDAIVVKQQITGKDALISLASPANRQGRQVADTISGISRRNQGGIGTAIIRTFGMTA
                     ASTGLSERTAKENELSFEVIHVSGKDHASYYPEATDILLKLIFHPETGEIYGAQGVGA
                     KGVDKRIDILATAIKGHLTIFDLPELELTYAPPFGSAKDPVNMLGYAAMNIAEGLSET
                     VQWHELPTELAKGKILLDVRTAEELEKGKFKEAKHIPLNELRDRLNELDSQQEYIVSC
                     HSGLRSYLAERILKQSGYHVKNLDGAFSLYQTVRPEELIYPNK"
     gene            complement(4384..4698)
                     /locus_tag="IXT20_001063"
     CDS             complement(4384..4698)
                     /locus_tag="IXT20_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HAQ4859525.1"
                     /translation="MNLLFQSIKSITVPELKEKLLENPALLDVRTPAEYRGGHIKGAK
                     NVPLQSINRYDGDKEKTVYVICQSGMRSKQAAKELKKSGYDVVNVRGGMNQWFDRTVG
                     GK"
     gene            4868..5128
                     /locus_tag="IXT20_001064"
     CDS             4868..5128
                     /locus_tag="IXT20_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HAQ4859526.1"
                     /translation="MEIDNKNILNRLKRTEGQIRGIQKMIEDEKECMDVITQLSAVRS
                     SIDRVMGMIVADNLKNCFEKPDSNPEEQAAKLEQAIKMIIKK"
     gene            5142..5456
                     /locus_tag="IXT20_001065"
     CDS             5142..5456
                     /locus_tag="IXT20_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur reduction protein DsrE"
                     /protein_id="HAQ4859527.1"
                     /translation="MKVVFHIDELEKWSETGKNVKNLIKASPETTIVVSVNGIAITGY
                     LDSANAEFLDLQGVVFHACANAMRANHISESSLPEQVIVVPAGVLDLVELQSQGYAYI
                     KP"
     gene            complement(5941..6495)
                     /locus_tag="IXT20_001066"
     CDS             complement(5941..6495)
                     /locus_tag="IXT20_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HAQ4859528.1"
                     /translation="MKLSTDWRKEIQTIPNLLSIFRIFLLPIYLYFVLRQSFYIAGAV
                     IVVSGLSDYLDGVIARRYNQVTDLGKVLDPFADKLTQLFLILSMAWYRPWLWLLFGLF
                     LIKEGFMFVAGLIGLSKNIKLSGAKWYGKVATAVIYVGMILLLLFPELPTLWVRVIFA
                     VITYGLLQSFVLYAVEYRKMFQRK"
     gene            complement(6621..8549)
                     /locus_tag="IXT20_001067"
     CDS             complement(6621..8549)
                     /locus_tag="IXT20_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HAQ4859529.1"
                     /translation="MDNEMISQLQFDRIKKEIQARAIGNYSKKRISDMTVSTNLQTVL
                     DRQEETREARLILESSQHVPFMGLPRIDALTEQVKKGLILQPTDLIEYADFLRSSRMI
                     TKFFDKNQYQTPLLFAYSKHLPDLINVEELIDQKIKNNKVSDDASRNLRKVRKQLQLI
                     EKEIQSKLLKFLRHPKNKEMIQEAMIVQKGEYYTIPIKASYKNKIDGTIIDESNKRTT
                     VFIEPTVISKLNEHYQLLKAEEISEEYQVLAALTGAIAENEEVIDLLIETITVLDIIF
                     ARAKFSREINGSTPRINKSEHIIIKQGRHPFLPEHAVPLDVEIGKDYRGLIITGANAG
                     GKTVVLKTVGLLTLMAMFGMQVPAKEGTELAVFDEIFVDVGDHQNLENALSTFSGHMQ
                     NIAAILKKIKRNTLVLLDEIGSGTEPNEGAALAIAIMESMYEQGALIIATTHYGEIKK
                     FAGDHEDFVPAAMAFDREALRPKYQLRVGETGESQALWIAHKMDMSMKLIHQAERYLA
                     EKAYRTQKKEFLPKTAVTNRKENKKERQLFAKGDRIFVNEYQKEALVYEDIGEDTIDV
                     YLDKKIIHVPRQRVRLVRSAEDLYPTGYDLDSLFIDYKTRKRQRDLERGSKKAHKVLV
                     KEMRKRQEERRVNDENSK"
     gene            complement(8855..9571)
                     /locus_tag="IXT20_001068"
     CDS             complement(8855..9571)
                     /locus_tag="IXT20_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HAQ4859530.1"
                     /translation="MNSDMTQIMGEFIGTLILVLLGDGVCAAVNLNKSKAQASGWIVI
                     AFGWGLAVTMAVYISGFMGPAHLNPAVSLAMAMTGAISWNLVVPFIIAQVLGAFAGAI
                     LVWLSYLPHWNATKDESAILGTFATGPAIRNYPANVITELIGTFVLVLGLLAFGQNEF
                     APGTNVFAVGGLILAIGLSLGGPTGYAINPARDFGPRLAHAVLPIANKGTSDWAYSWV
                     PIAGPMIGAIIAVGVYSLMV"
     gene            complement(9572..11395)
                     /gene="glpO"
                     /locus_tag="IXT20_001069"
     CDS             complement(9572..11395)
                     /gene="glpO"
                     /locus_tag="IXT20_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glycerol-3-phosphate oxidase"
                     /protein_id="HAQ4859531.1"
                     /translation="MFSNKTRQDSIQKMQQEELDLLIIGGGITGAGVAVQSAASGIKT
                     GLIEMQDFAEGTSSRSTKLVHGGIRYLKTFDVEVVADTVGERAVVQSIAPHIPKPDPM
                     LLPIYEGEGATTFNMFSVKVAMDLYDKLANVTGTKYENYTLTPEEVLEREPFLKKEGL
                     KGAGVYLDFRNNDARLVIDNIKKAAEDGAYLVSKMKAVGFLYDGDQIVGVKARDLLTD
                     EVIEIKAKLVINTSGPWVDKVRNLNFTRPVSPKMRPTKGIHLVVDAKKLPVPQPTYFD
                     TGKQDGRMVFAIPRENKTYFGTTDTDYQGDFTDPKVTQEDVDYLLDVINHRYPEANIT
                     LNDIEASWAGLRPLLIGNSGSDYNGGDNGSISDKSFNKVVDTVSEYKENKVSRAEVED
                     VLNHLENSRDEKAPSTISRGSSLEREPDGLLTLSGGKITDYRKMAEGALRLIRQLLKE
                     EYGIETKEIDSKKYQISGGNFDPTKLEETVTELAKEGVAAGLEEEDATYIADFYGTNA
                     RRIFELAKEMAPYPGLSLAESARLRYGLEEEMVLAPGDYLIRRTNHLLFERDQLDEIK
                     QPVIDAIAGYFEWTEEEKAQQTKRLEALIAESDLRELKGEK"
     gene            complement(11409..12905)
                     /gene="glpK"
                     /locus_tag="IXT20_001070"
     CDS             complement(11409..12905)
                     /gene="glpK"
                     /locus_tag="IXT20_001070"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HAQ4859532.1"
                     /translation="MTESTYIMAIDQGTTSSRAIIFDKKGRHIGSSQKEFTQYFPQES
                     WVEHDANEIWNSVQSVIAGAFIESGIKPNQIAGIGITNQRETTVIWEKDTGRPIYHAV
                     VWQSRQSAGIADNLKNEGYQEFFHEKTGLVIDAYFSATKIRWILDHVEGAQERAEKGE
                     LMFGTIDSWLVWKLTDGQAHVTDYSNASRTMLFNIHQLDWDQEILDLLNIPRVMLPKV
                     TSNSEVYGYTQGYHFYGSEAPISGMAGDQQAALFGQMAFEPGMVKNTYGTGSFIVMNT
                     GEKPQLSKNNLLTTIGYGINGKVYYALEGSIFVAGSSIQWLRDGLQMLQKASDSEDAA
                     KRSTSEDEVYVVPAFVGLGAPYWDQAARGAMFGLTRGTTKEDIIKATLQSIAYQVRDI
                     IDTMQDDTGIKIPVLKVDGGAANNEYLMQFQTDILNVPIQRAENLETTALGAAFLAGL
                     AVGYWKDTDEIREFYEAGKLFEVQMEEERREKLYNGWKKAVKATQAFE"
     gene            complement(13041..14501)
                     /locus_tag="IXT20_001071"
     CDS             complement(13041..14501)
                     /locus_tag="IXT20_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAQ4859533.1"
                     /translation="MKIEALLERKEQLQILILRNLVLQGGTASINGLREHVQLSKASF
                     DQYLEDIELIGRMMEKKVEVQRNEYQALLVLDEDVSLEKILLFLLQESLKFKMLVYLL
                     EHQQVSIVRLATAFNISESSVFRKIKELNQLLEEFGLQIKNGQLYGEELQIRYFYYEL
                     FQYIPEDQRPLFLQNTPEKRPFILGLDRVLETTFTASAEAQIACWLGITKKRLLNEKS
                     TYATLKEKKLLYQSDRLYQAIDPIIVMHLSRTAAELNVYETMMFYSFFVSFSIVDEEI
                     FYQYDLTRSKKLPTAVLDTYIRETMLWHYRPRRLKIKEEKAVGYQISQINNEWFFFVG
                     KIEVYERDRLLEQQQKMLGHSLTQLLAKLQETAVQQLPRKRAEDSELSYLMIQYANVL
                     LMIDFYIAKPVVIGIDLESLPIYRIAFQQYLIRELRGIGGIEIGSYEEGKEYDLVITF
                     CQRNKKQCEYYLSEFASPYDIIRLKRRIETLKKEKN"
     gene            complement(14491..15786)
                     /locus_tag="IXT20_001072"
     CDS             complement(14491..15786)
                     /locus_tag="IXT20_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAQ4859534.1"
                     /translation="MNIVIVGGSFAGIHAAIYLRQLMPTSEIYLIEKQSKLGWIPSGF
                     NLILKGKVTSEKQLSWITKEELTDRYRIHVYTEKTVIGLKEKKVVLAGGKKLDFDRLI
                     LATGSNQKFRNISAESTLIHPVKKIQNVTALQQKIMNAKKIAIIGAGQAGIEIAEGLA
                     SRKKQIHLYESRKSILFRYLDPEMTEPLVKEMKNQGLSLFLEEQVVSLTENETAIVET
                     EKRKENYDLVLLANHSRPDNQMWEEQLTLNDDGTIWVDDYLQTSQKDVYAIGDAIQVT
                     FRPTNEKMYVSLVNNAVRTARNVSKTISGSQTKDQGTYRPVGNQWFGYFLGSVGLTEE
                     ESIFYPQKIETGYVDYKLTAINQSKVKLKFLFDSEKKILGAQLQSKKEMFHLLDQLTI
                     AVKEGWTFEQLEEHELFFQPEYRVPDMVWSKVANTAYED"
     gene            complement(16118..16795)
                     /locus_tag="IXT20_001073"
     CDS             complement(16118..16795)
                     /locus_tag="IXT20_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HAQ4859535.1"
                     /translation="MKVLIVLTNVEKYEKIERPTGLWLSELTHFYDVLVKNGIDADFV
                     SPKGGYVPLEPQSVLNMDEIDWAYYEDEDFRNRALGNTLRPDDVDANDYAAVYYAGGH
                     GTMWDFPEAIDLGKVASQIYRNEGLVSAVCHGVVGLLPVTDESGKSILAGRTVTGFSN
                     EEEEANGTTNEVPFLAEDALKEKGANYQSGAAFSEVVQIDGRLLTGQNPQSAHQLGEA
                     VVEALKN"
     gene            complement(16825..17916)
                     /locus_tag="IXT20_001074"
     CDS             complement(16825..17916)
                     /locus_tag="IXT20_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent flavin oxidoreductase"
                     /protein_id="HAQ4859536.1"
                     /translation="MSQFDQSITLPSGVQLKNRLMLSPMTTQLSFFNGVITEDEITYY
                     KQRSKGLGAVITGAANVQDIGKGWPGELSIAHDEMIPRLSELAKAIQGEGAKAIIQIF
                     HGGRMTSRATLSGEQPVSASAVAAKRPDAETPRAMSEEEIVETIQAFGKATRRAIESG
                     FDGIELHGANTYLIQQFFSPHSNRREDQWGGSLENRYRFIEELLTTVFSAVEQYAKKP
                     FIVGYRFSPEEYETPGIRFEDTIWLLERLRESKLDYLHVSLNMYDRIARSDKYNDKSI
                     LEVVHNTIQGKIPLVGVGGIRNREDVEQVLKYAELAAIGQQMIVDPDWAEKLLENRED
                     EFVTKPFEEAYKELYLPSPLYNFLKARYQ"
     gene            complement(18017..18451)
                     /locus_tag="IXT20_001075"
     CDS             complement(18017..18451)
                     /locus_tag="IXT20_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAQ4859537.1"
                     /translation="MEKPIEKALDECLFFSVKKLDRMLNKLADEAFRRTGLAPTYGFI
                     LLILKEKDGIPQKDIAQMLYSAPSTIARFVEKLEYKGYVKTVSEGRLSLVYLTEQGRQ
                     FAKEVDGSWEELHQSYKAVLGNEASKELAQTMNDATDKLQKK"
     gene            complement(19035..20492)
                     /locus_tag="IXT20_001076"
     CDS             complement(19035..20492)
                     /locus_tag="IXT20_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAQ4859538.1"
                     /translation="MKKFPEGFLWGGATAANQCEGAWDVDEKGLSVSDVYTFDSDLPK
                     EKWTDQWHMMTHQQVKAAQDPNSKYYYPKRHGNDFYHHFKEDIRLFAEMGFKCYRMSI
                     AWTRIFPHGDEETPNEAGLFFYDQVFDECLKYGIEPVVSLSHYEMPLYLVTEYGGWPN
                     RKLIQFYVRFAETVFKRYKNKVKYWMTFNEINCVKHHPFVSVGVIEENHPHLEQAKYQ
                     GAHHQFIASALATKACHEIIKGSQVGCMISYQLLVPYSCDPDDIQKTIEQQRTSLFFS
                     DVQARGYYPAYTQRMFEEKGVNLKIEVGDEEILAAYPVDFVSFSYYMSSAVSAHPENL
                     EGVQGNLITGGIKNPYLPSSEWGWQIDPKGLRIALNQLYDRYQKPLFIAENGLGAIDK
                     VEEDGTVQDDYRIDYLKQHIEQMKEAIEDGVDLFGYTSWGCIDIVSASTSQMSKRYGY
                     IYVDQDDEGNGTKKRLKKKSFDWYRQVIQSNGEIL"
     gene            complement(20489..22384)
                     /locus_tag="IXT20_001077"
     CDS             complement(20489..22384)
                     /locus_tag="IXT20_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ4859539.1"
                     /translation="MEKPFSKNYTYLVRRIATALINKGEVKMDYSQLAKDIVRFVGGE
                     ENVSNVYHCATRLRFTLKDNKKADKEKVEQLEGVITVVEAGGMFQVVVGNAVNEVYDV
                     LSKQMKLEDDASSGKRGTEKKGILNSFIDMIAAVFAPTLGVLAGSGLIKGVLALCTSL
                     NLLTTESGTYIILNAAADAFFYFLPIFLAYTAAKKFNTDRFIAMVIAAALVYPTIVSA
                     YSDSITLRFLGMPVILARYTSTVIPAILAVWVLSYIEPKIRKSLHESIRNLLTPFICI
                     IVMVPITLLVVGPIADYASQLIAAGYLAVYNFSPVLSGAVIGGFWQVLVIFGLHWGLV
                     PVMTNNLSFYGRDTLGPACMTAVAAQAGAVLGVFLKTKNKKVKSLSLSAFITALFGIT
                     EPAVYGVTLKYKRPFYIACICGAIFGGVAGAAGAGALAVATRSILSFPIYIGEGFVWL
                     VASYFLAMISSCMLTFLFGYKDEIEEESSKDIVLSTPAAGEIIDLSEVNDPTFASGSL
                     GEGFAIIPTDGKIYSPVNGEVSTVFPTKHAIGVVSEEGAEILIHIGIDTVNLNGKYFQ
                     SAVSDGKKVRKGDLLMEVDLQELIKEGYDPTTMVIVTNSSRFKNIITSHKKNNQTRKL
                     ASLGGSI"
     gene            complement(22442..24217)
                     /locus_tag="IXT20_001078"
     CDS             complement(22442..24217)
                     /locus_tag="IXT20_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAQ4859540.1"
                     /translation="MFITKRRCKQMDLNKRQLQILVVLRNKNHWITSEELAEALGTNK
                     KTIQTEIKNMLAIYEKKIIIQSNKRSGYFLESIESETKQQIIQEVTKHKIYSSMDFRA
                     STIVTYLLFQKGYVSMQKIADIFFLSKTAVSLQIKTILRWIERNPKLELEVSSTKGLK
                     IIAEENSKRIFLSLVGTETVIQHARLPQQISTIFSNIMPTVQKTLKEVLISYNYIVSG
                     EDFIRFSRYLVLTILRREELLEKTNRYTLFIPMVETLTKKLSSELPYSFSNQEKQAIE
                     NRLLELNYLMINDEKNKEIEKNLRAFEQTIIRFLDLPVSLLFHETEFLLTHIKQMKTR
                     MDAGHNTMNHFVQKIISRYPLEIYLIRRFFPIHFQLRPNLAESAYLVLYLAEALNPFR
                     KQGEILIVSNQPISILNTLKKQIQQSMNMWIKECRIEPVYLFKNQSNKITEYDAILTT
                     EQELIFEWDDLIYLPSVLDEFESSEIYQILREKLTIVEEKKKTKLLDHYYKEENQIKI
                     HKKIDSIQQLISPSSDLVMLPLNAETLLCCLIKSQVETKITEYQLEIPLIHQQRKIKR
                     VFFVSFDYKRNDILVFFQAVSDLLG"
     gene            complement(24267..25763)
                     /locus_tag="IXT20_001079"
     CDS             complement(24267..25763)
                     /locus_tag="IXT20_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAQ4859541.1"
                     /translation="MDQEIFPENFLWGGAVAANQCEGAWLEDGKLPNVTDTLIGIMNQ
                     HPSIQWNEEKKIWEIALDESLHYLSHEAIDFYHRFEEDIRLLKELGLKAFRTSISWAR
                     IFPRGDEQKPNEAGLVFYDRLINTLNRYDIEPVITLSHYETPLALVGEYGGWQNRKLI
                     DFFEFYAQTVFERYQGKVKYWMTFNEINNAFRMPYAAAGLVTFPARDKLEPIATLTKK
                     EIYQACHHMFLANARATQLLKKIDPNAKMGIMCSFSALATYAYDCDPENVFGSLQFKR
                     NSWFFSDVMCRGHYPAYIYRIWKEEDCAPVMLDGDEQYLQANTVDYIAFSYYRSAVYK
                     KEAEMRVDTGGANGFDNPFLKEKSPEPWSWPIDPLGFRYVMNELTDRYELPLFIVENG
                     IGLDEAPDELNRIHDPKRCEYLKIHLQEIAEAIKDGCQIIGYLWWGPIDIVSAGTGEM
                     RKRYGFIYVDRQNDGSGSFRRLKKDSFEYYKQIIQSNGKNLEYRSFFK"
     gene            complement(25957..27579)
                     /locus_tag="IXT20_001080"
     CDS             complement(25957..27579)
                     /locus_tag="IXT20_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HAQ4859542.1"
                     /translation="MEQKWWHHSVVYQIYPRSFQDSNQDGIGDLRGIISRLDYLAYLG
                     IDAIWLSPVYESPNDDNGYDISNYQAIMAEFGTMEEMEELIEEAKKRNIRIIMDLVVN
                     HTSDEHPWFIEARKNKENPYRDYYIWRDPVNGKEPNRLRSIFSGSAWQLDEKTGQYFL
                     HLYSKRQPDLNWKNKQVRQEIYQMMNFWIDKGIGGFRMDVIDMIGKEPDKEITSNGPK
                     LHEYLQEMNQATFGDKNLMTVGETWGATTEIAKLYSNPKRNELSMVFQFEHIGLDQQE
                     GKDKWDLKPLSISELKKVLSKWQTSLGDEGWNSLFWNNHDLPRIVSRWGNDDVYRKES
                     AKMFAILLHMMKGTPYIYQGEELGMTNCPIDDIAEAKDIETINMYNERISSGFTKEEI
                     LESINAKGRDNARTPMQWNAREHAGFTTGIPWLRVNPNYKEINAEEALADQDSVFYMY
                     KKLIDLRRKHDIIVWGDYELVEKTPEHVFAYSRIYEDQQWLVICNMSSEKQTFTVPRE
                     ALRVIVSNYPLKQLPDGKVDLRPYEAFVLEVK"
     gene            complement(28029..29342)
                     /locus_tag="IXT20_001081"
     CDS             complement(28029..29342)
                     /locus_tag="IXT20_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HAQ4859543.1"
                     /translation="MKEKILSYFEIEKLNTTVKREVLAGFTTFISMAYILFVNPTVLG
                     ASGMDEGAVFTATALASAIGCILMGVLARYPIGTAPALGINAFFAYSVCLGMGIPWET
                     ALAGVFVASLIFILITIFKLREMIIDAIPADLKYAISGGIGLFIAFLGLSEGGIIVSN
                     ESTLVGLGSLNVGSTWLTIFGLVVTAILLVRRVPGGIFIGMAATTILGLVTGLIPMPS
                     EIVAAAPSLKPTFLVALKHVGDINSLQMWVVVLTFLLVTFFDTAGTLVGLANQAGFMK
                     DNKMPRVGKALASDSTAMLAGSLLGTSPVGAFVESSAGIAVGGRSGITAITTGIFFLL
                     GLFFSPLLSVVTSQVTAPALIMVGVLMAQSLRQIEWGNLEIAIPSFLILIGMPLTYSI
                     SDGIALGFIFYPITMLAAKRGKEVSPIMYGLFFVFMGFMWILNVK"
     gene            complement(29587..29811)
                     /locus_tag="IXT20_001082"
     CDS             complement(29587..29811)
                     /locus_tag="IXT20_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4859544.1"
                     /translation="MDLMGRLEGYLKYRRAKHSEKLRAKNLKSGKKKTKGWGKMVDTG
                     FGGLNNLNPVDTKEFIKEEQKRNGKIKSHY"
     gene            complement(29831..30199)
                     /locus_tag="IXT20_001083"
     CDS             complement(29831..30199)
                     /locus_tag="IXT20_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HAQ4859545.1"
                     /translation="MIQKTRVMLYVNDVEMICRFFVEKIGAEISETIELPEEFKSIVL
                     SISKELELGVFPKVFVQKFSPEVLGPPPSLVFFTDEFETIYENMEEPGEITDNNGVLT
                     FNFSDPEGNYFVIGKAESSD"
     gene            complement(30395..31105)
                     /locus_tag="IXT20_001084"
     CDS             complement(30395..31105)
                     /locus_tag="IXT20_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4859546.1"
                     /translation="MKKVIVDTNFWEVFPEATIEILSVNGIDNHVTKESEETYHQLLN
                     EAAKEARNYLTEETFSQNEVIAQWRQAFTTFKTKKGARSSIEALLKRANQEREFFPIN
                     PLVDIYNSISLSFGVPCGGEDIEKIAGNLHLGKAQGDESFLPLGADSDAPALPEEIIY
                     YDEQGAICRCLNWREAQRTMLTEETQAAVLVIESINAEQAARGAQAIEALKEKIDQTF
                     GVTSVRHTLTKDESEAEL"
     gene            complement(31123..31619)
                     /locus_tag="IXT20_001085"
                     /pseudo
     CDS             complement(31123..31619)
                     /locus_tag="IXT20_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321138.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            complement(31656..32108)
                     /locus_tag="IXT20_001086"
     CDS             complement(31656..32108)
                     /locus_tag="IXT20_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003331879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAQ4859547.1"
                     /translation="MAEILRDIGMIARALDSISNIEFKEVDLTRGQYLYLVRICENPG
                     IIQEKLAEMIKVDRTTTARAIKKLESNGMIERLEDKENKKIKKLYPTKKGAEIYPFII
                     RENNYSNAVALNGLSDEEAKQLEYLLNKVCKNVSEDWNFVKKGNKRIY"
     gene            complement(32243..32848)
                     /locus_tag="IXT20_001087"
     CDS             complement(32243..32848)
                     /locus_tag="IXT20_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HAQ4859548.1"
                     /translation="MNKKLAQYKRLLALSQAGLYYGKDVFDKERFEEIRTISLELISE
                     IGKETFEEIKALTTIGEGHPTPKVDVRAYIKKDGKVLLIEDKRTKEWSLPGGFAEIGL
                     SPEENVRKEVYEETGLTVETTQLRAVFDTNKQKDIPQLFQYYKLVFACAIHGEEAKFI
                     ENNETSNMGFFSIEELPKLSEKRTTKKQLLILENKNQIYCD"
     gene            complement(33298..33918)
                     /locus_tag="IXT20_001088"
     CDS             complement(33298..33918)
                     /locus_tag="IXT20_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAQ4859549.1"
                     /translation="MKKYFILLAGSPGTGKTYLMNKLRERFPDMYAITLDEIKEYYAE
                     SIGFNNLEERAEQERTKVYPFYYKALDLYMEAGKKVIVSEYPFSDKQKARLQELADRY
                     GYQVITIRLTADFEVLWQRRYKRDRESDRHLSYIMDHYHYGDVLEDRTKGTNHITKEA
                     FKKIIADRKYDKFQLGTLYEFDVTDYTQVDYTPLLDKLEKEINDNK"
     gene            complement(33920..34789)
                     /locus_tag="IXT20_001089"
     CDS             complement(33920..34789)
                     /locus_tag="IXT20_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase family
                     protein"
                     /protein_id="HAQ4859550.1"
                     /translation="MYTTLKEVTKTAEELNFTVGAFNTHNLEMLPAMLGAAKEMGSPI
                     IIQTSVDTAKYIGMHVLVNVMTAIAEEEMVDAVLHLDHARDFDDIKEAIDSGYTSVMY
                     DGSHLPFKENILKTKAVVEYAHAHGVSVEGELGTIGGTEEGIHVAENDKVYTRPEDAV
                     KFVEATGVDALAIAIGTNHGQFKSKTEVNIPLLKEIDAVVDVPLVIHGGTGVKEEDYP
                     ELINNGIRKFNVGTELLVNWTKVAKETFSETEINKSLRHNVIPANKAVKEIVKHKIGL
                     FMNLESPINLGNK"
     gene            complement(34812..35219)
                     /locus_tag="IXT20_001090"
     CDS             complement(34812..35219)
                     /locus_tag="IXT20_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HAQ4859551.1"
                     /translation="MFGFFKKKEKEPKKVLAKEEKALSPEATEKIQSEITQLKQEIST
                     TQDRHKLAELYEQVGLKFSELHENDQAIQYLEKSLENKQTIGDGYKKLMSLYNQKRAD
                     AARAGDDQGIDYYMGKMDEMRQIAKQVTIKGNK"
     gene            complement(35238..36527)
                     /locus_tag="IXT20_001091"
     CDS             complement(35238..36527)
                     /locus_tag="IXT20_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018378547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ4859552.1"
                     /translation="MSVINFIIENILTQASITIALIAMLGLLLQKKSAGQVISGTLKT
                     LLGFQVLSAGSSIIVGSLTYFGKIFTEGFHMQGIIPSIESINDQAMNDLGLGRDIALT
                     FLAIFVFNIILARFTKWKYIFLTGQAILWMATMTTVFGYFAGLRGIVLILVGGFIGAC
                     FAIAMPAVAQPIIRKITGSNDIALGHFCTIGYLFEAGVAKLFGEKGENKKSIEDIKLP
                     THFEFLQDTYLSVMVVMVPLYIITVLFAGEPFASELSGDQNYIMFAFLQAIQFVVGVY
                     ILLAGVRLLLGEIVPAFRGIAMKLVPDAIPALDCPVFFPYSPNAVILGFITTTIGTII
                     AMFTLPMFGLAMILPGMLTNFFAGGTAGIFGNAVGGRRGAIIGGIAHGFFITLLPALL
                     VTIFNSMGFINATATDVDTVAAALLYAWILSPVLKAF"
     gene            complement(36554..>36767)
                     /locus_tag="IXT20_001092"
     CDS             complement(36554..>36767)
                     /locus_tag="IXT20_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ4859553.1"
                     /translation="LGVEAKLDHCDISSAVSMKPDVIITAENFRSQFEKFAVDPKTAM
                     IYLKNIVSKNEIEEKITPVLQDKGVI"
BASE COUNT        10422 a         7890 c         6120 g        12335 t
ORIGIN      
        1 attcaaaaga tttatttact tttttgtata gatttcgatt gttccaccta gtcaatacaa
       61 atgaggtaat caggaaactg aaaaaaatca atacaaaaaa gccgattaag aaatacagca
      121 ttttcttttc tgataaaaaa gcgtacaagg aaaaatcctt ggctctttct ggtctcgtta
      181 atgggaaaaa gacccatata aaaggaagaa caacgatcat ccatcctaaa agagccacta
      241 acgtttgcca aatctttaac cagtaattac cttttgaaaa atacttatct ttcaccaaaa
      301 ttgatgcaac ttcttctctt gttacttttt tcatatcctc acctcacgtt tttttgttac
      361 gacgtaggag tttaccatac atttcatttc ttttcacata aagtgttcct aaatttttcc
      421 taagaaaaag aacaaaacat ttctcatcag caaatgaaaa atattttgtt ctttttatca
      481 gttgttcttt ttatttttta tctcttaatt atgttgctct ttccaatacg acaatgcttt
      541 tcattcctct agatatcgcc gtgtacattt gtctgcgtct tctttgctca tctccatcgt
      601 tttcaaatgg atataagatt acatgatcaa attccagtcc ttttgctaga ctcaaaggaa
      661 tgatttggca gttttctaat ccatatgaat tttttttaat cgattcttgt agcgtttggg
      721 tttcctcttc tgtttgggtg ataactgccg ctttttctcc ttttagagaa accaaaatct
      781 cagctagtat tttcaacaat tcttcagagg atcgaaaaga gcggtattcg ggttcttttc
      841 cttgcggtcg gatcgatacg atgtcgatct tctcttgatc cacagcatag gttttgaaaa
      901 gctctgtgat tgctccgcta gaacggtaac tatttctaag atcataccga tggatcggca
      961 gatggtagtc atcgaaacaa cgcataatat cagcaaaagt agtggaagaa ttaaagattg
     1021 cctgattctc gtcacctact aaagtaaagt gagtttttgg aaataattcg atcaatagac
     1081 ctaattgtgc cagggtataa tcttgtactt catcaatcaa tagatagcga aatgctggta
     1141 catttacctt ttcaacaagt aaatgacgaa tagctaatat aatgactgct tcatcaacag
     1201 tacgtgtggg ttgccaagca taagctgagc catatcgttt ttcataaatt ctttcaaaca
     1261 actgatgctc atccacccat gccatttctt cgatttgtct ggttattttt cgatattttt
     1321 tttgtaaaag tttccttgca taagctacaa tactttgttc cgaatcatca gaaatcaatt
     1381 taccgaataa ttccaactgc ctatcttctg tcaacgagag tacctgattc tgcacagcag
     1441 gactttttgc ttgtttcaaa agatgattct tccattcgct catcagtgcc tttttcgttg
     1501 cttgaagcct atcgatcaat ttaggatgag caggaacttt ctcatatatt ttttcgatcg
     1561 tttttttaga aaggactgtt tttccatttt tagtaagtcc acggataaaa ttcggatgat
     1621 ctaaaaacaa aggatctgat tgttttagat tatcgataaa ctttttgcta cgcaaacgtt
     1681 ctgtctgctc cgatacattt tctcccgtga tacgtttgaa ataagcctct tcaccttcta
     1741 tttcctctgc tgaaagctgt gaaaccagct gtatgatggt tagattcagt ggatttcttt
     1801 cacctaatga tggtaggaca ttcgaaatat agtcgataaa ccgatgatta ggtgatagaa
     1861 tcagcaagtc atcggatgtc atcgtatctc gatataaata gagcagatac gcaatacgct
     1921 gcatgatagc agaggtttta ccacttcctg ctacgccatt taccaagata ttttgacttt
     1981 tcacatcccg aatgattgtg ttttgttccg attggatcgt agcagtgata gccttcattt
     2041 cattggtctc atcttggcct aagacttcta ataatacgtc atcttgaata gctacagacg
     2101 tatcaaaaaa cttgaccaaa cgatctcttt ccaaaagaaa ttgtcgtctt tctttaataa
     2161 ccgtatcgat tttattttga cgaaccgtat aagaagatgg accaatcatg ttattgtaaa
     2221 aaagttcggc aattggtgaa cgccaatcat aaacaagatt ttcatctgca tcatctgtga
     2281 aatttgctgt accgatataa aaggattctt ttggctcatt atccagaaag tctacaacga
     2341 cttttccaaa gtagggactt ttcaataaac gttcgatctt tgctagctga ttttctgcgt
     2401 ttttctgttt gatattcatc tgatcgattt ctctattttt catttcaatc atcgaaaaag
     2461 tatccaggtt atctaagtaa ctgtcaaaat tcaatcgaag ctcgccagcc atttctttca
     2521 acgactggtt tccttctatt tttgccgtat ccatcgattc ccgcagttcg ttttttttat
     2581 caattagctt ttgatacacg ttcttcagat atgcttcttc taactgtttt tgtgtgttga
     2641 ccatacacat tctcctcttt ttttaaagtg gtggaaccga ttagtttacc tcttttaaga
     2701 gtcgaatgca agctttttaa cgagtatcat ttgttaggat atatcagttc ttcaggtcgg
     2761 acagtttgat ataaagaaaa tgcaccatca aggtttttta cgtggtagcc agactgcttc
     2821 aagattcttt ccgctagata gctacgtaat ccgctatgac agctgacgat atattcttgt
     2881 tggctgtcta attcatttaa tcggtcccga agttcattca aagggatatg tttggcttcc
     2941 ttgaatttgc ccttttccaa ttcttctgct gttcgcacat ctaataaaat ttttcctttt
     3001 gctagttctg tcggcaattc atgccattgt acggtttcac taagcccttc tgcaatgttc
     3061 attgctgcat atcctagcat gtttactgga tctttggctg agccaaatgg cggtgcatac
     3121 gtcaattcta attccggtaa atcgaagatc gtcaaatgcc ctttgattgc tgttgctaaa
     3181 atatcgatcc gtttatccac accttttgcc ccaacacctt gtgcaccata aatctcgcca
     3241 gtctctggat ggaagatcaa cttcagtaaa atatctgttg cttctggata atagcttgca
     3301 tgatcttttc ctgatacatg aatgacttca aaagacagtt cgttttcttt ggctgttctt
     3361 tcacttaaac cggtggatgc ggcagtcatt ccaaacgttc gtataattgc tgttccaata
     3421 ccgccttgat ttcttcgaga aattccggaa atcgtgtccg caacttgtct accttgacga
     3481 ttggctggtg aagcaagaga aataagtgca tcttttccag tgatttgctg ttttacaacg
     3541 atagcatccc ccaccgcata aatatcagga atattcgttt gataacgttc gtctactagg
     3601 atacccccgc gtatgcctaa ttcgattcca gcggtcactg ctagctggtt atcaggacga
     3661 acaccaatgg ataaaataac taaatcagca gggatggtac gcccatcatc cagtcgcaat
     3721 cgtttcccgt ggtcttcaaa tccagcgaca gcatgagaag taatcacttc tactccttga
     3781 tgagacaatt ctttttctat aaaagctgcc atttcttcat ccagtgttgg aagaatatgt
     3841 ggtgcttgtt cgatcaccat gacttctaaa cctcttcttt ttaggttttc tgccatttcc
     3901 aatccgatga atcctgcacc aacgatcacg gcacgctttg gctgtttttc taatgcatgc
     3961 atcactttat cgatatctgg tacatttcta atagaaaaaa cattgtcggc ttctgttatc
     4021 cctggaatcg atggaacaac tggttttgca ccaggagata aaaccaatgc atcgtactgt
     4081 tcaatgtgtt tttgaccctc atgtacgact tctaccgttt tgttttcggg aaagatggct
     4141 gtcacttcat gatttggacg cacatctaaa caaaaacgag cggataatga ttctggcgtt
     4201 tggacaagaa gattttcccg ttcactgatt tctcccgaaa gataataagg caagccgcaa
     4261 tttgcaaaag aaacatacgg tcctttttca aaaacaacga tttctgcatc ttccattaat
     4321 cggcgaagcc gtgtagcagc agacattcca cctgctacac ctccgacaat aacgattttc
     4381 atcttatttc cctcctactg ttcgatcaaa ccattgattc atgccaccac gtacattcac
     4441 tacatcataa ccagatttct ttaattcttt agctgcttgt ttactcctca tccctgattg
     4501 acaaatcaca tagacggtct tttctttgtc tccatcatat ctgtttatac tttgtaatgg
     4561 aacattttta gctcctttta tatgcccgcc ccggtattct gcaggtgttc taacatcaag
     4621 taacgctgga ttttccaaaa gcttttcctt taattctggg actgttatcg attttattga
     4681 ttgaaatagc aaattcattt tctgcacctc atttacattt ttataaatac ctcatggggt
     4741 atattaaaca ggagctttat taatatgtca accagaatga ctcttcattt ttttcactac
     4801 tttttatatt tgcataaaat accgataagg ggtattatct aattataaaa atagaggaga
     4861 atgtgttatg gagattgata ataaaaatat cctgaaccgt ttgaaacgta cagagggaca
     4921 aattcgtggt atccaaaaaa tgatcgaaga cgaaaaagaa tgtatggatg tcatcactca
     4981 attaagcgct gttcgttcta gtattgatcg cgtaatgggc atgatcgttg cagataatct
     5041 gaaaaattgc tttgagaaac ctgatagtaa tccagaagaa caagcggcta aactagaaca
     5101 agcgatcaag atgattatta aaaagtaaag gaatgatttc aatgaaagtc gttttccata
     5161 ttgatgaatt agaaaaatgg tcagaaacag ggaaaaacgt aaaaaacctg ataaaagctt
     5221 ctccagaaac gacgattgtc gtatctgtaa atggcatcgc gattaccggt tatctagact
     5281 ctgccaatgc agaattctta gatttgcaag gagtcgtttt tcacgcttgc gccaatgcta
     5341 tgcgtgctaa tcatatttca gaaagttctt tacctgaaca agtaatcgtt gtaccagcag
     5401 gtgtccttga tttagttgaa ctgcaatccc aaggttatgc ttatatcaag ccttagtcag
     5461 tagaggttgc gacagaagtg atcagcttca agaaatttcg gcttattttt cgaagaagct
     5521 acttctggaa aaccgtgaat caaaggttgt gaaaacgctg tcctgcatca agtactaaga
     5581 acagccaaac aaaaatagct tctcatattt ttcgtttggt tgggcttatt accgcagatg
     5641 caagcttttg aacaccgttt aaggaagagg ttgtgaaatc agcggtttga cctaagtatt
     5701 agagaaaaaa acgggaaaat agctcctcat attttgccgt tttttgagct aataccgaag
     5761 gtcagctgat tgaacaccgt ttaccaagag gttgtgagcc taccatttaa ctccgaatca
     5821 caagaaataa tttcaaaaac aacttctcct gtttttggtt tggttggctt attatcgaga
     5881 agtagctact gggagtctat ttatcaaaaa aaggaatgcg acataggtca cacccctttt
     5941 ttattttctt tgaaacatct ttcggtactc gacagcatac aagacaaaag attgtaaaag
     6001 accgtaagtg atgacggcaa aaatgactct cacccacagc gttggtaatt ctgggaaaag
     6061 cagcaaaagg atcatgccaa catagatgac agcagtcgct actttgccat accacttcgc
     6121 tccacttaat ttgatatttt tagatagccc gattaatcca gctacaaaca taaagccttc
     6181 ttttatcaag aatagaccaa acaacagcca aagccacggt cgataccatg ccatagacaa
     6241 aatcagaaaa agttgtgtca atttatctgc aaacggatca agcaccttcc ctaagtctgt
     6301 tacctgatta tatctccgag caatgacccc gtctagataa tcagacaaac ctgagacaac
     6361 aatgaccgct cctgctatat aaaatgactg tctcagcaca aaataaagat aaatcggcag
     6421 caaaaaaata cggaatatcg ataaaagatt cggaatcgtt tgtatttctt tacgccagtc
     6481 tgtgcttaat ttcattttat cgccctacct cctcagcact cttttctttt tattttagct
     6541 tacacgggga ataaaaccaa tgatttcact gtttttattt ttctttacag ccactgatat
     6601 tatgagctaa tttgagataa tcacttgctg ttttcatcgt ttactcttct ttcttcctgt
     6661 cgtttgcgca tttcttttac tagtacttta tgagcttttt ttgatcctcg ctctaggtca
     6721 cgctgccgtt tacgtgtttt ataatcgata aacagactgt ctaaatcgta acctgtcggg
     6781 tacagatctt ctgctgaacg caccagacga acacgctgac gaggcacgtg gattattttt
     6841 ttgtctaaat atacatcaat cgtatcttca cctatatctt cataaactaa cgcttctttt
     6901 tggtattcat tgacaaatat ccgatcacct tttgcaaaga gctggcgttc ttttttattc
     6961 tccttcctat ttgtaactgc tgtcttagga agaaattctt ttttctgtgt ccgataggct
     7021 ttttccgcca agtaacgctc tgcttgatgg atcaatttca tcgacatatc cattttatga
     7081 gcaatccaca aagcttggct ttctcctgtt tctccaaccc gcaactgata tttaggacga
     7141 agtgcttccc gatcgaaagc catagcagcc ggcacaaaat cttcatgatc tccggcgaat
     7201 ttcttgattt ctccataatg ggtcgtggca atgatcaatg ccccttgctc atacatagat
     7261 tccatgattg cgatcgctag tgcagcccct tcatttggct ctgttccact gccaatctca
     7321 tctaaaagta ccaacgtatt cctttttatc tttttcaaaa tagcagcgat gttttgcata
     7381 tgaccagaaa atgtactcaa cgcattttct aaattttggt ggtcaccgac atcgacgaaa
     7441 atctcatcaa agacagcaag ctctgtccct tcttttgccg gaacttgcat accgaacatc
     7501 gccatcaaag tcaataaacc gactgtcttc aacacgactg ttttgcctcc ggcatttgct
     7561 ccggtgataa tcaagcctcg atagtcttta ccgatttcaa catccaatgg taccgcatgc
     7621 tcaggtaaaa atggatgtct gccttgcttg ataatgatat gttccgattt attgattcta
     7681 ggtgtgcttc cattgatttc tcggctaaat tttgcccgag caaagatgat atctaacacc
     7741 gtgatcgttt cgatcaataa atcgatcact tcttcgtttt ctgcgatagc accagtcaat
     7801 gccgccagga cttgatattc ttcactgatt tcttcagctt ttaacaactg atagtgttca
     7861 ttcaatttag aaatcacagt tggttcaata aaaaccgtcg ttcttttatt tgattcatcg
     7921 ataatcgttc catctatttt atttttatag ctggctttga taggaatcgt atagtattcg
     7981 cctttttgaa caatcattgc ttcttggatc atttctttgt ttttcggatg acgcaagaat
     8041 ttcagtaatt ttgactggat ttctttttcg attagttgta attgtttgcg tactttacgc
     8101 aaattacgtg acgcgtcgtc actgacttta ttatttttta ttttctggtc aatcaactct
     8161 tctacattta tcaaatcagg aagatgtttg ctataagcaa acaacaaggg tgtctggtat
     8221 tgattcttat caaaaaactt tgtgatcatc cgactgcttc taagaaaatc cgcatattcg
     8281 atcaaatcgg ttggctgcag aatcaaccct tttttgactt gttctgtcag tgcatcgatc
     8341 ctcggcaacc ccataaatgg aacatgctga ctgctttcta agatcaagcg tgcttctctt
     8401 gtttcttctt gacgatccaa gacagtttgt aggtttgtcg agaccgtcat atcgctgatt
     8461 cgttttttgc tatagtttcc aattgctctt gcttggattt cctttttgat ccgatcaaat
     8521 tgtaattgac taatcatttc gttgtccata ttctctcctt ttcttgaaga gatacctgtc
     8581 ccaaaataaa taagggccta gagataaata aaaggataga acacagctta aaaaagtcat
     8641 gtcctatcct tattagaata caaagatatt gttttcctga caggtaccgt ttcataagca
     8701 caccaaataa actttgttta ttggctattc gacttactga ttagatacca gctacactca
     8761 caaaacatac cccattcttc taggtcttat ttattttcca cttaactata gcaagacaca
     8821 ttttggttgt caaactttag tatgttttat agatttacac cattaatgaa tagacaccta
     8881 ccgcgataat tgctccgatc attggacctg cgattggtac ccatgaatat gcccaatctg
     8941 aagttccttt attagctatt ggcaataccg catgtgctaa tcgaggacca aagtcccgag
     9001 cagggttgat tgcatatcct gttggccctc ctaatgaaag accgatggct aagatcaatc
     9061 cacctacagc gaatacgttc gttccaggtg caaattcgtt ttgtccaaaa gctagcaacc
     9121 caagaactaa tacaaatgta ccaattaatt ctgtgatcac atttgctgga taattccgta
     9181 tagcaggacc tgttgcaaac gttcctaaaa tcgcgctttc atctttcgtt gcattccaat
     9241 gtggcaaata agaaagccaa acaagaatgg cccctgcaaa agctcctaaa acttgcgcaa
     9301 tgatgaaagg aacgacaaga ttccaactaa ttgcccctgt catcgccata gctagagaga
     9361 ctgcaggatt caaatgagct gggcccatga atccagaaat atagacagcc attgttacag
     9421 cgagtcccca accaaatgcg atgacaatcc agccagaagc ctgtgcttta cttttgttta
     9481 aattcacagc cgcacacaca ccatctccaa gcaaaactag gatcagtgtc ccaataaact
     9541 cccccatgat ttgagtcata tcactgttca tttatttctc cccctttagt tcccgcagat
     9601 ctgattctgc aatcaatgct tctaaacgtt tagtctgttg cgccttctcc tcttctgtcc
     9661 attcaaaata tccagcaatt gcatcgatca caggttgctt gatctcatca agctgatctc
     9721 gttcaaacaa cagatgattt gtacgacgaa tgagataatc gcctggggcc aaaaccattt
     9781 cttcttctaa accataacgt aaccgagctg actcagcgag actcaaacca ggataaggtg
     9841 ccatttcttt tgctaattca aagatacgtc gggcattagt cccgtaaaaa tcagcgatat
     9901 aagtagcatc ttcttcctct aaaccggctg ctactccttc ttttgctaat tctgtcactg
     9961 tttcttctaa tttagttgga tcgaagtttc cacctgaaat ctgatatttt ttagagtcga
    10021 tttctttcgt ctctattccg tattcttctt ttaataattg gcgtatcaat cgcaaagctc
    10081 cttctgccat cttacggtaa tcggtgattt tcccaccaga taaagtcagc aaaccatctg
    10141 gttctctttc taaagaacta cctctggaaa tcgtagaagg tgctttttca tcacggctgt
    10201 tttccaaatg gttcaacacg tcttctactt cagcacgaga aactttgttt tccttatatt
    10261 cacttactgt atcgacaact ttattgaagc tcttgtctga aatcgatcca ttatctccac
    10321 cattgtaatc agaaccagaa ttaccaatca acagtggacg aagtcctgcc cagcttgctt
    10381 cgatatcatt caatgtgata tttgcttctg gatagcgatg attgatcaca tccaatagat
    10441 aatctacatc ttcttgtgtg actttcggat ccgtaaaatc tccttggtaa tccgtatctg
    10501 tcgtaccaaa gtaagtcttg ttttctcttg gaatagcaaa aaccatccgc ccatcttgct
    10561 ttcctgtatc gaagtatgtg ggttgcggta caggcagttt tttcgcatct acaactaaat
    10621 ggatcccttt agttggacgc attttaggag agactggacg cgtaaaattc aagtttcgta
    10681 ctttatctac ccaaggacca ctcgtattga tcactaattt tgctttgatt tcgatcactt
    10741 catctgtcag caggtcacga gctttgacgc caacgatttg atcgccatca tataaaaagc
    10801 caaccgcttt cattttactt actagataag ccccatcttc ggcagccttt ttgatattat
    10861 caatcactaa acgggcatca ttgttgcgga aatctagata gacacccgcg ccttttagcc
    10921 cttctttttt caaaaacggt tctctttcca atacttcttc tggtgtcagc gtataattct
    10981 catatttggt tcctgtcaca tttgccagtt tgtcataaag gtccattgct actttgaccg
    11041 agaacatatt gaaggttgtt gctccttcgc cctcatagat tggcaaaagc attggatctg
    11101 gttttgggat gtgtggggca atactttgta cgactgcacg ttcaccaact gtgtcagcta
    11161 ctacttccac atcaaatgtc ttcagataac gaataccgcc atgaacaagt ttggtcgagc
    11221 gagaggaagt cccttctgca aaatcctgca tttcgatcaa tcctgttttg attcctgatg
    11281 ctgctgactg gactgccaca ccggcaccag tgattccgcc accgatgatc aacagatcca
    11341 attcttcttg ctgcattttt tgaatgctat cttgtcttgt cttgtttgaa aacatatgga
    11401 tttcctcctt attcaaatgc ttgggtagct tttactgctt ttttccaacc gttgtaaagt
    11461 ttttctcggc gctcttcctc catttgaact tcaaatagct tgccagcttc ataaaattcg
    11521 cggatctcgt ctgtatcttt ccaataaccg acagctaatc ctgctaaaaa tgctgcccct
    11581 agcgccgttg tttccaagtt ttctgctcgt tggatcggta cattcaagat atctgtttgg
    11641 aattgcatca gatattcatt gtttgctgca ccgccatcta ctttcaatac aggaatcttg
    11701 attccagtgt catcttgcat cgtatcgatg atatctctca cttgataagc aatcgactgt
    11761 aaagtagctt taatgatatc ttcttttgtc gtaccacgtg tcaagccaaa catagcccct
    11821 cgtgctgcct gatcccaata aggagcacct agcccaacga atgcaggaac aacataaacc
    11881 tcatcttcgc ttgtagagcg tttcgctgcg tcttctgaat cgctagcttt ttgcaacatt
    11941 tgaagtccat ctctcagcca ttgaatcgaa gaaccagcca caaaaatact tccttctaaa
    12001 gcatagtaaa cctttccatt tatgccgtaa ccaattgtcg tcaataaatt atttttggat
    12061 aattgcggtt tttcaccggt attcatgaca ataaatgaac ctgtaccata agtattcttg
    12121 accatgcctg gttcgaatgc catttgtccg aataatgcgg cttgctggtc tcctgccatc
    12181 cctgaaattg gtgcttcgct tccataaaaa tgataacctt gcgtataacc ataaacttct
    12241 gagttagaag tgacttttgg taacatcact cgaggaatat ttagtaaatc taaaatctcc
    12301 tgatcccaat ctagctgatg gatattgaac aacatcgtcc gactggcatt cgagtaatca
    12361 gtaacatgtg cttgtccatc ggtcagtttc catactaacc aagaatcgat cgttccaaac
    12421 atcaattcgc ctttttcagc tcgttcttgc gcgccttcta catgatcaag aatccagcgt
    12481 atcttcgttg ctgagaaata ggcatcgatc actaaccctg ttttttcgtg gaaaaattct
    12541 tgatagcctt cattttttaa gttatctgca attccagctg actgtctgga ttgccaaaca
    12601 actgcatgat agattggacg tcctgtatct ttttcccata ttactgttgt ttcacgctgg
    12661 ttcgttatgc caatcccagc aatctgattt ggtttgatcc ctgattcgat gaacgctccg
    12721 gcaataactg actgtacaga attccagatt tcatttgcat catgttccac ccaactttct
    12781 tgcgggaaat attgcgtgaa ctctttttga gaactgccga tgtgtcttcc tttcttgtca
    12841 aagataattg ctcgtgagct agttgtacct tggtcaatcg ccatgatata cgttgattct
    12901 gtcattgtga ttccctccat tatatgcaaa cgctttcttt aactactctt atcatactac
    12961 tgttttaaaa aaagaaactg tcaaaatttt attctttttt tctttttttg acagtttttt
    13021 attatgaagc tattttttaa tcaattcttt tcttttttca gcgtctctat tcttcttttc
    13081 aatcgaataa tgtcgtaagg tgacgcaaat tctgacagat aatattcaca ctgttttttg
    13141 tttctctggc aaaatgtgat caccaaatca tattctttac cttcttcata acttccaatc
    13201 tcaataccgc cgattcccct cagttctctg atcagatatt gttgaaaagc aatacgataa
    13261 atcggcagac tttctaaatc gattccaatc actactggtt ttgcaatata aaaatcgatc
    13321 atcaaaagaa cattggcata ctggatcatc aggtaactaa gctcactatc ttcagctctt
    13381 ttcctcggca attgctgcac cgctgtttcc tgtaatttcg ccagtaactg tgttaaggaa
    13441 tgcccgagca ttttctgctg ctgttccaaa agccgatcac gctcatagac ttcgatcttt
    13501 ccaacaaaga aaaaccattc attgttgatc tgggaaattt ggtagcctac tgccttttct
    13561 tctttgattt tcaaacgacg aggacgataa tgccataaca tcgtttcccg aatgtaagta
    13621 tctaaaacag cggttggtaa ctttttgctc cgtgtcaaat catactgata aaatatttct
    13681 tcgtctacta tcgaaaaaga aacaaaaaag ctgtaaaaca tcattgtttc gtatacattc
    13741 agttctgctg ctgttcgact aagatgcatc acgatgattg gatcgattgc ttgataaagc
    13801 cggtccgact gatagagtaa tttcttttct ttcaaagttg cgtacgtact tttttcattc
    13861 aatagtcttt ttttcgtaat cccgagccag caagcaatct gtgcttctgc tgatgccgta
    13921 aacgttgttt ctaatacacg gtccaatcct agaatgaacg gtcttttttc tggtgtattt
    13981 tgcaaaaaaa gcggtcgctg atcttccggg atatattgga atagttcata ataaaaatat
    14041 cgtatctgta gctcttctcc gtataattga ccgtttttga tttgtaatcc aaattcctct
    14101 aagagctggt tcaattcttt gatctttcga aaaacagaag actcactgat attaaacgct
    14161 gtcgctaatc gaacgatgga tacttgctga tgttctaata aatagacgag catcttaaac
    14221 ttcaagcttt cctgcaataa aaacaataga attttttcca aacttacatc ttcgtccaac
    14281 acaagcagcg cctgatactc atttcgctgt acttctactt ttttttccat cattcggcca
    14341 atcaattcaa tatcttccaa atattgatca aacgaggctt ttgacagttg gacatgctct
    14401 ctcaaaccat tgatagaggc tgttccccct tgcagaacaa ggtttcgcaa aatcaggatc
    14461 tgtaactgct cttttctttc taataatgct tcaatcttca taggcagtat tcgccacctt
    14521 tgaccacacc atatctggca cacggtattc tggctggaaa aatagctcat gttcttctaa
    14581 ctgttcaaac gtccatcctt cttttactgc aattgtcagt tggtcaagaa ggtggaacat
    14641 ctcttttttg ctttggagct gtgctccaag aatctttttt tcagaatcaa atagaaattt
    14701 gagtttaact ttgctttgat ttattgctgt aagcttgtag tctacgtatc ctgtttcaat
    14761 tttttgggga taaaaaatgc tctcttcttc tgtcaaacct acgcttccta agaaatagcc
    14821 aaaccactga ttgccgactg gacgataggt tccctgatcc tttgtctgtg agccactaat
    14881 tgtcttgctg acatttcgtg ctgtacggac agcgttattt actagagaaa catacatttt
    14941 ttcatttgtt ggacgaaaag ttacttggat cgcatcgcca atcgcataaa cgtctttttg
    15001 agacgtttgc aagtagtcat ctacccagat cgttccatca tcattcaatg ttaattgttc
    15061 ttcccacatc tgattgtcgg ggcgagaatg attagccagc aaaacaagat catagttctc
    15121 ctttcttttt tccgtctcta caatagcggt ttcattttcg gtcaaagaca caacttgttc
    15181 ttctaaaaaa agtgaaagcc cttgattttt catttccttt actaacggtt ctgtcatttc
    15241 aggatctaga taacgaaaaa gaattgactt tcggctttcg taaagatgga tttgtttttt
    15301 tcttgatgcc aacccttcag ctatttcgat tcctgcttgt cctgcaccaa taattgcaat
    15361 tttctttgca ttcatgatct tttgctgaag agccgttacg ttttgtatct ttttaactgg
    15421 gtgaataagc gttgattctg cagaaatatt tcgaaacttt tgatttgaac cagtcgctag
    15481 aatcaaacga tcaaaatcca atttttttcc tcctgctaaa acgacttttt tttctttcaa
    15541 tcctattact gtcttttctg tgtaaacatg gattcgatag cggtcagtca gttcttcttt
    15601 cgtgatccaa gaaagttgtt tttcactggt aacctttcct tttaatatca aattgaaccc
    15661 gctgggaatc catcctaact tcgattgctt ttcaatcaaa tatatttcac ttgttggcat
    15721 caattggcga agataaatag ccgcatgaat ccctgcaaac gacccgccga caattacaat
    15781 gttcatctgc attcctcttt cttataaggt gttctactgc taacttctca atcagaaaac
    15841 acagtattca taactcgctt tcaaccttat gatacatagc gatgcagcag aatcaaccct
    15901 ctccatcata ccgtgttcta ggtctgcttc tcaataatgt actagtggac ctaattgaag
    15961 agtatcattt ctgaagagat gtttcaaaaa atgtactctt ataaaagaaa aaaactcgcc
    16021 ttttacgtac ctctgtccaa ataggaaaca gaaaagaacg aaaagacgag ttttagcgtg
    16081 tgtaatgtaa tagttagaat agacacatca tttattttta attctttaat gcttcaacaa
    16141 ctgcttcgcc taattgatga gcagattgag gattttgccc tgtaagaagc cgcccatcaa
    16201 tttgaaccac ttctgaaaaa gctgctccag actgatagtt tgctcctttt tctttcaaag
    16261 catcttctgc taagaatggt acttcattcg ttgtaccatt tgcttcctct tcttcattac
    16321 tgaacccagt cactgtacgt ccagctaaaa tagattttcc agactcatct gtaactggca
    16381 acaatcctac aacaccgtga caaacagctg agaccagccc ttcatttcga tagatttgtg
    16441 aagcgacttt cccaagatct attgcttctg gaaaatccca cattgttccg tggccaccag
    16501 cataataaac cgctgcataa tcatttgcat ccacatcatc aggacgtaag gtatttccca
    16561 aggcccgatt tctgaaatct tcatcttcat aataggccca atcgatttca tccatattca
    16621 aaacactttg cggttctaaa ggtacatatc cgcctttagg actgacaaaa tctgcatcga
    16681 tcccattttt tactaataca tcataaaaat gagtcagttc acttaaccac aaacctgtag
    16741 gacgctcgat cttttcgtac ttttctacat ttgttaatac gatcaatact ttcattttcc
    16801 catcctcttt ctataaaaat tgcgttattg ataacgtgct ttcaagaaat tatataatgg
    16861 gcttggcaaa taaagctctt tatatgcttc ttcaaaaggt ttcgttacaa attcatcctc
    16921 acggttttcc aataatttct ctgcccagtc aggatcgacg atcatttgct gtccaattgc
    16981 tgcaagttct gcatatttca atacctgctc tacatcttcg cgattgcgga tcccaccgac
    17041 accaactaaa gggatctttc cttgaatcgt gttatggacc acttcaagaa tcgatttatc
    17101 gttgtattta tcagaacgag ctattcgatc atacatattt aatgaaacat gcagataatc
    17161 tagttttgac tcacgtaatc gttcaagaag ccagatcgtg tcttcaaaac ggatacctgg
    17221 tgtttcatat tcttctggtg agaaacgata ccctacgatg aatggttttt tagcatattg
    17281 ttcaaccgct gagaaaaccg ttgttaataa ttcttcaata aaacgataac gattttccaa
    17341 gctaccgccc cattgatcct ctcgtctatt cgaatgcgga gagaaaaatt gttggatcaa
    17401 atacgtattt gctccatgca actcgattcc atcaaatcct gattcaatcg ctctacgtgt
    17461 ggctttgcca aatgcttgga ttgtttcaac gatttcttct tcactcattg ctcgtggtgt
    17521 ttccgcatca ggacgttttg ctgcaactgc acttgcagat acaggctgtt ctccagataa
    17581 agttgctcga cttgtcattc gtccaccgtg gaaaatctga ataatcgctt ttgcgccttc
    17641 tccttggatt gctttcgcta gctcacttaa tcgaggaatc atttcatcat gagcgatgct
    17701 gagttcacct ggccagcctt ttccgatatc ttggacattt gcagcaccag taataacagc
    17761 accaagacct ttagaacgtt gtttataata agtgatctca tcttcggtga tcactccatt
    17821 gaaaaagctg agttgtgtcg tcataggcga cagcatcaat cggttcttca attgaactcc
    17881 tgaaggtaaa gtaattgatt gatcaaattg tgacattgtc attcctccca taattcgctc
    17941 tgattaacct atcgagaatc atctatcatt aaaccactcg attagttgta catgcaacta
    18001 atttgcttaa aaaattttat tttttttgta acttatctgt tgcatcattc attgtctggg
    18061 ctaattcttt acttgcttca tttcccaaaa ctgctttgta tgactgatga agttcttccc
    18121 agctaccatc gacttcctta gcaaattggc gtccttgttc tgtaagataa actaatgaca
    18181 agcgaccttc gctaactgtt ttgacgtatc ctttatattc taatttttca acaaaacgag
    18241 caatcgttga tggggcggaa tagagcattt gtgcaatatc tttttgtggt attccatctt
    18301 tttcttttaa tatcaataaa ataaaaccat aagttggtgc cagacctgtc cttctaaacg
    18361 cttcatcagc taatttattc aacatgcgat ctagcttttt cacgctaaag aaaagacatt
    18421 catctaacgc tttttcaatt ggcttttcca tttcatcccc tccattggcg tacatatcat
    18481 agtatgattt ttattttttg tcaaaaaaca cggaataatg tccgcctcct ctgtctagat
    18541 ttgaaaaaaa gaagtagcca tcttcaaaga atttcggctt gttttcgaca aaactccttc
    18601 tggaacaccg tgaatcagag gttgtgaaaa agtcgtttag ttttgagtat gaagaaaaat
    18661 tttgtgaaaa tggctttcca catttttgca taaatttggc ttaataccga aaaactggct
    18721 tttgaacacc gtttatatca gaggttgtga aaaagccgtc ctgcatcaag taataagaac
    18781 agccaaacaa aaatagttcc tcatattttg tcgttttttg agctaatgcc gatggtcagc
    18841 tgattgtaca ccgtttattg agaggtcgtg agcctgccgt tgaactccga gtcacaagga
    18901 gcaatttcaa aaaacagctt ctcatgtttt atgactatgg tgacttgtga tcgaggagtt
    18961 ggcaggagaa tactgtttat tcgtaaagaa ggggctgtga caagactttt gtcacagtcc
    19021 cttatttcct tttattatag gatttcgcca tttgattgga tgacttgtct gtaccaatca
    19081 aatgattttt tctttaaccg tttctttgtg ccattccctt cgtcatcttg atcaacatag
    19141 atatatccat aacgttttga catttgtgaa gtagaagcgc tgacgatatc aatgcagccc
    19201 cacgacgtat aaccaaataa atctactcca tcttcgatag cttccttcat ctgctcaata
    19261 tgctgcttca aatagtcaat gcggtaatcg tcttgaacgg ttccatcttc ttctactttg
    19321 tctatagctc cgagtccgtt ttcagctatg aacagtggtt tttgatacct atcatataat
    19381 tgattcaaag caattctcaa acccttagga tcaatctgcc agccccattc acttgatgga
    19441 agatacggat ttttaattcc tccagttatt aagtttcctt ggaccccttc caaattttct
    19501 ggatgagcac tgactgcact agacatgtag tagctaaaag aaacaaaatc aacagggtag
    19561 gctgctaaga tttcttcatc acctacttct atttttaagt tgacaccttt ttcttcaaac
    19621 attcgttgcg tatacgctgg ataatatcct cttgcttgaa catcactaaa gaacaaagaa
    19681 gttctttgct gctcaattgt tttttgaata tcatccggat cacaagaata cggaacaaga
    19741 agctgatagc tgatcataca accgacttgt gaacctttga taatttcatg acaagctttt
    19801 gtagctaaag cacttgctat aaactgatgg tgtgcacctt ggtatttcgc ttgttccaaa
    19861 tggggatggt tttcttcaat tactcctacg ctaacaaaag ggtgatgttt tacacagttg
    19921 atctcattga aagtcatcca atattttact ttatttttat agcgtttgaa tactgtttca
    19981 gcgaaacgaa cgtaaaactg aataagcttt cgattaggcc atccaccata ttctgtaacc
    20041 aaatataacg gcatttcata atgggaaaga gaaacgactg gctcaatccc atattttaga
    20101 cattcgtcaa acacttgatc ataaaaaaat aatcccgctt cattgggtgt ttcttcatca
    20161 ccatgaggaa aaattcttgt ccatgcgata gacatccgat agcatttaaa acccatttca
    20221 gcaaataaac ggatgtcttc tttaaagtga tgatagaaat catttccgtg tcttttagga
    20281 taataatact tgctgttggg atcctgcgcc gcttttactt gctgatgcgt catcatatgc
    20341 cattgatctg tccatttttc tttaggtaaa tcgctgtcga atgtatacac atcagacaca
    20401 gataatcctt tttcatctac atcccatgca ccttcacatt gattggccgc tgtcgctccg
    20461 ccccataaaa atccttctgg aaattttttc atatcgatcc ccctaaactt gctagttttc
    20521 ttgtttgatt atttttctta tgactagtaa tgatattttt gaatctacta ctatttgtaa
    20581 caataaccat tgtggtcgga tcatatccct ctttgattaa ttcttgcaaa tctacttcca
    20641 tcaataaatc gcctttacgt actttttttc catcagatac tgcactttga aagtatttgc
    20701 catttaaatt cactgtatca ataccgatat ggatcagtat ctctgctcct tcttcactga
    20761 ctaccccaat tgcatgtttc gttggaaaaa ctgttgaaac ttccccgttt acaggactgt
    20821 agatttttcc gtccgttgga atgatcgcaa acccttctcc taaagatcca ctagcaaatg
    20881 ttggatcgtt tacttcagat aaatcaatta tttcacctgc tgcaggagta cttaacacta
    20941 tatcctttga ggattcttct tcgatttcat ccttgtagcc gaaaagaaaa gttagcatac
    21001 aggaactgat catcgctaag aaataactag caactagcca aacaaatcct tcaccaatat
    21061 aaatcgggaa agacaaaata cttcttgtag caactgccaa agcaccggct cctgcggcac
    21121 cagctactcc accaaatata gctccacata tacaagcaat gtaaaatggt cgtttatatt
    21181 taagagtcac accataaaca gcaggttcag tgattccaaa taatgctgtg ataaatgcag
    21241 ataaagataa actttttact ttcttatttt tcgttttcaa aaatacccca agtactgctc
    21301 ctgcttgcgc agctacggca gtcatacatg ctggacctag cgtatcacga ccgtagaaag
    21361 aaagattgtt ggtcattact ggtacaagtc cccaatgtaa cccaaaaatc actaacactt
    21421 gccaaaatcc gccgataaca gcaccagata aaacagggct aaagttataa acagctaaat
    21481 atccagctgc aatcagctga cttgcataat ctgcaattgg acctacaact aataaagtaa
    21541 ttgggaccat cacgatgata caaatgaatg gtgtcaataa atttcgaata ctttcgtgca
    21601 agcttttgcg aatcttaggc tcaatataag aaagaaccca tacagctaaa attgctggaa
    21661 taaccgtact tgtatatctt gctagaatca cgggcatccc tagaaatcgc aaggttatgc
    21721 tatcactata tgcactgaca atcgtaggat aaacgagagc tgctgcaata accatagcga
    21781 tgaaacgatc tgtattgaac ttttttgcag cagtatatgc caaaaatata ggcaagaaat
    21841 agaaaaatgc atcagctgct gcattcaaaa tgatataagt tcctgattca gtagttaata
    21901 aattcaaact cgtacataag gcgagtactc ctttgatcaa accacttcct gccaagacgc
    21961 ctaaagtcgg ggcaaaaaca gcggcgatca tgtctataaa agagtttaaa atgccttttt
    22021 tctcagttcc tctctttcca ctagatgcat catcttctag tttcatttgt ttggataata
    22081 cgtcatacac ttcatttact gcatttccta ctacgacttg aaacattcct ccagcttcga
    22141 ctacagtgat cacaccttct agttgctcta ccttctcctt atctgccttc ttattatctt
    22201 ttagggtaaa gcgtaacctt gttgcacaat ggtaaacatt gctgacattt tcttcgcctc
    22261 cgacaaatcg gacaatatct ttcgctaatt gtgaatagtc catttttact tcccccttgt
    22321 taattaatgc cgtagcaatc cttcttacaa gataagtata gttcttagaa aacggtttct
    22381 ccattcaatt agtacccctt ctaatagcga acgaaactgt acaatagtgt gtatggttta
    22441 cttagccgag caaatcactc actgcttgaa agaaaacaag tatatcgttt ctcttatagt
    22501 caaaggagac aaaaaaaaca cgcttgatct tcctttgctg atgtataagt ggtatctcaa
    22561 gctgatactc tgtgattttt gtttctactt ggcttttgat aagacagcac aacaaagtct
    22621 cggcatttaa cggaagcata actaggtcag aactaggaga gattagttgc tgaatggaat
    22681 ctatcttttt atggattttt atttgatttt cttctttgta gtaatgatca agcagttttg
    22741 ttttcttttt ttcttcaact atggtcagct tttcacgcaa aatctgataa atctccgaag
    22801 attcaaactc atctagaaca ctaggtagat aaatcaaatc atcccactca aaaataagct
    22861 cttgctcagt agtcaaaatt gcatcatatt ccgttatttt gtttgattga tttttgaaaa
    22921 gatacactgg ttctatccgg cattctttga tccacatgtt catactttgt tgaatctgtt
    22981 tttttagtgt attcaaaata ctaattggct ggttactaac aatcagtatc tccccttgtt
    23041 ttcgaaaagg attcaacgct tcggcaagat acaaaaccaa ataagcgctt tcagctaaat
    23101 tgggacgtaa ttgaaaatgt attgggaaga aacgccgaat taaataaatt tctaaaggat
    23161 aacgggagat gatcttttgt acaaaatgat tcatggtatt atgtccggca tccatcctcg
    23221 ttttcatttg tttgatatgg gttagcaaaa attctgtttc atggaacaat aacgaaacag
    23281 gaagatctaa aaaacgaata atcgtttgct caaatgctct tagattcttc tctatttctt
    23341 tgtttttttc atcattaatc atcaaataat ttaactctaa tagtcgattt tcaattgctt
    23401 gtttttcttg attggaaaaa gaatagggta gttctgatga taactttttt gtgagggtct
    23461 ctaccatcgg aataaataat gtatacctgt ttgttttttc taaaagttct tctcttcgaa
    23521 gaatggtcaa aacgagatac cgagaaaatc gtatgaagtc ctctcctgaa acaatataat
    23581 tatatgatat aagaacctct tttaatgtct tttggacagt cggcatgata ttggaaaaaa
    23641 ttgtactgat ttgttgaggc aaccttgcgt gttggatcac cgtttctgtt ccaacaagag
    23701 ataagaaaat tctttttgaa ttttcttcag caatgatttt tagtcctttt gtcgacgaaa
    23761 cttccagttc taatttagga ttgcgctcga tccaacgcaa aatcgttttt atttgtaatg
    23821 atacggctgt ttttgaaaga aagaaaatgt ctgctatctt ttgcatactg acataccctt
    23881 tttgaaacaa taagtacgtc acaatagttg aagctcggaa atccatgctg gaatagattt
    23941 tatgtttcgt cacctcttgt atgatttgct gtttagtttc agattcaatt gattctaaaa
    24001 aataacctga acgtttattt gactggataa tgattttctt ctcgtagata gcaagcatat
    24061 ttttgatttc agtttggatc gttttcttgt tggtacctaa agcttcagct aattcttcgc
    24121 ttgttatcca atgattctta tttcttaata ctacaagaat ttggagttgt cgtttattta
    24181 agtccatttg cttacacctc ctctttgtta taaacataca ggaaaaacct ctcaaatgac
    24241 aatagtcact tgcagaggtt taaaaatcat ttgaagaaag atctatattc aaggttttta
    24301 ccattagact ggataatttg tttataatat tcaaaactat cttttttcaa tctcctaaag
    24361 cttccgctcc catcattttg tctatccaca taaataaagc catatcgctt cctcatttcc
    24421 cctgttcctg ccgatacgat atcgattggt ccccaccata agtaaccaat gatttggcaa
    24481 ccatctttta ttgcctcagc aatttcctga agatgtattt ttagatactc acaacgtttt
    24541 ggatcgtgga ttcgattcaa ttcatctggt gcttcatcta gtccaatgcc attttcaact
    24601 atgaataatg gtaattcata acggtctgtc aattcattca tgacataacg aaaccctaga
    24661 ggatcaattg gccaactcca aggttcggga gatttttcct tcaaaaaggg attatcgaaa
    24721 ccatttgctc cgccagtatc tacgcgcatt tctgcttctt ttttatagac agcgctacga
    24781 taataactaa atgcaatata atctaccgtg ttagcttgca agtactgttc gtctccatct
    24841 aacattactg gtgcacagtc ttcttccttc catatcctgt aaatataagc tggataatgc
    24901 cctcggcaca tgacatcgga gaaaaaccat gaattgcgtt taaattgcag tgaaccaaat
    24961 acattctctg gatcacagtc atatgcataa gtcgctaatg cagaaaagct acacataata
    25021 cccatcttag catttggatc gattttcttt aataattgag tagcacgggc gttcgctaga
    25081 aacatatgat gacaggcttg ataaatctct ttttttgtta gtgtagcaat gggttcaagt
    25141 ttatctctag cagggaatgt aaccaatcca gcagctgcat aaggcatacg aaaagcatta
    25201 ttgatctcat taaaggtcat ccaatatttc actttccctt ggtagcgttc aaagacggtc
    25261 tgcgcataaa attcaaaaaa gtcgattaat tttcgatttt gccacccccc atactctcct
    25321 accaatgcta agggggtttc ataatgacta agagtaatta ctggctcgat atcatatcga
    25381 tttaatgtat tgattagtct gtcataaaat acgagaccag cctcatttgg cttctgttcg
    25441 tctcctcttg gaaatattcg tgcccatgag atgcttgttc gaaaagcttt taaacctaac
    25501 tccttcagta aacgaatatc ctcttcaaat cgatgataaa aatcaatcgc ttcatgagaa
    25561 agataatgga gtgattcgtc taaagcaatc tcccaaatct ttttttcttc attccactga
    25621 atactaggat gctggttcat gatcccgata agcgtatctg tcacattagg taatttgccg
    25681 tcttccaacc atgcaccttc acattgatta gcagctactg ctccgcccca taaaaaattt
    25741 tctggaaata tttcctgatc cataactatc tctcctcgct atcaacaatt tcttacttga
    25801 agtatagctc gattcatcaa aacaagatat tctaactatt tcggcagaag agaagaaaaa
    25861 aattgaatgc tactcttcat ttttaatatt tatatataac aaatacataa gacgatcccc
    25921 gaaagaaaac actcgttaac catctaagca aacgatttat tttacttcca acacaaatgc
    25981 ttcataaggt cgtaaatcaa ctttgccgtc tggtaactgt ttcagaggat aatttgacac
    26041 aataaccctt aaagcttcac gtggaacagt gaacgtctgc ttttcactgg acatattaca
    26101 aataacgagc cattgctggt cttcatatat gcgagaatag gcaaagacat gctcaggcgt
    26161 cttttccact aattcatagt ctccccagac gatgatgtca tgttttctac gcaaatcgat
    26221 caatttcttg tacatataaa aaacagaatc ttgatctgct aacgcttctt ctgcattgat
    26281 ttctttataa tttggattga ctcgaagcca tggaattcca gtagtaaacc cagcatgttc
    26341 acgggcgttc cattgcatag gcgtcctagc attgtctctt cctttcgcat tgattgattc
    26401 caagatttcc tcttttgtaa atccagaaga aatcctttcg ttatacatat tgattgtctc
    26461 aatatctttt gcttccgcga tatcatcgat tgggcaattc gtcatgccta actcttctcc
    26521 ttgatagata taaggggtgc ctttcatcat atggagcaag atagcaaaca tcttggcact
    26581 ttcttttcga tacacatcgt catttcccca acgagaaaca attcgcggaa gatcgtgatt
    26641 attccaaaat aaactattcc atccttcatc acctaaagat gtttgccatt tggataatac
    26701 ttttttcagt tcagagatac tcaagggttt caaatcccat ttgtctttgc cttcttgctg
    26761 atctaatcct atgtgttcga attggaagac catggataat tcatttcttt taggattgga
    26821 ataaagttta gcgatttcgg tagttgctcc ccatgtttcg ccaacagtca tcagattttt
    26881 gtcaccaaaa gttgcttgat tcatttcctg aagatactca tgcagtttag gtccattact
    26941 tgtgatttct ttatccggtt ctttgccaat catatcgatc acgtccatac gaaagccacc
    27001 aataccttta tcgatccaga aattcatcat ttgataaatc tcttggcgaa cctgtttatt
    27061 tttccaattc aaatcaggtt gacgcttgct atacaagtgc agaaaatatt gccccgtttt
    27121 ttcatccaac tgccaagcag aaccactgaa tattgagcgc aatctattag gctcttttcc
    27181 attgaccgga tcacgccaaa tataatagtc ccgataagga ttttctttat ttttacgggc
    27241 ctcaatgaac cacggatgtt catcagaagt atgattcacc accagatcca tgatgatacg
    27301 gatatttctt ttctttgctt cttcaatcag ttcttccatt tcttccatcg taccgaattc
    27361 tgccatgatt gcttgataat tactgatatc atacccattg tcatcattcg gagactcgta
    27421 tactggagaa agccaaattg catcaatacc taaatacgcc agataatcca acctggaaat
    27481 aatcccccgc aaatctccaa taccatcttg atttgaatct tgaaaacttc ttggatagat
    27541 ttgataaaca actgaatggt gccaccattt ttgttccatg attttcctcc tataaatgga
    27601 tgtttctatt ttttgcttaa tcatttattt tagtctctct cactaaaaat tgtaacgctt
    27661 tcaaatagct attgcacctt gttatcggta acagaataaa acaaaaaaac agctgaggaa
    27721 attccctcaa ctgttttttg aaaggattga tttattcagg gaagaatgct gatttcacaa
    27781 cttctttata aacggtgttc actcagctgt ccctcggtat tagctcaaaa aacggcaaaa
    27841 tataagaaac tatttttttc gtttttttct ctaatactca ggccagaaca ctgatttcac
    27901 aacctcttta taaacggtgt tcactcagct gtcccttggt attagctcaa aaaaaggcaa
    27961 aatatgagga actatttttt cgtttttttc tctaatactc aggccagaac actgatttca
    28021 caacctcttt atttcacatt caatatccac atgaatccca tgaatacaaa gaacagacca
    28081 tacatgatgg gagatacttc ttttcctctt ttagctgcta acatggtaat tggatagaag
    28141 ataaaaccta atgcaatccc atctgaaata ctataagtca aaggcatgcc gatcaagatc
    28201 aagaatgaag ggatcgcaat ctctaagttt ccccattcga tttgtcgcaa agattgtgcc
    28261 atcaatactc ctaccataat caatgctggt gcagttactt gtgaagtaac aactgatagt
    28321 aatggagaaa agaacaagcc taacaagaag aaaatccctg tagtaatagc tgtgatacct
    28381 gaacgtcctc caacggcgat accagctgat gattctacaa atgcaccaac aggagaagta
    28441 cctaataagg aacccgcaag catagctgtt gaatcagaag ctaaagcttt ccctacacga
    28501 ggcatcttgt tgtctttcat gaaacctgct tggtttgcta atccaactaa agttcctgct
    28561 gtatcaaaga aagtcacaag caagaatgtc agcactacga cccacatttg aagagaattg
    28621 atgtccccta catgttttaa tgcaactaaa aatgttggtt tcaaacttgg tgctgcggca
    28681 acaatttcag aaggcattgg aatcaaacct gtgaccagac ctaaaatcgt tgtagctgcc
    28741 atcccgatga agatgccacc tggtacacgc cgaacaagca agatagcagt tacgactaat
    28801 ccaaaaatcg tcaaccaagt agaaccaacg ttgagtgatc ctaatcctac taatgtagat
    28861 tcattcgaaa cgatgatccc accttcgctc agaccaagga aagcaataaa tagaccgata
    28921 ccgccagaaa tcgcatattt caaatctgct gggatcgcat caatgatcat ttcccgcagc
    28981 ttgaagattg tgatcaaaat aaaaatcaaa gaagcaacga atacgccggc taatgctgtt
    29041 tcccatggaa tccccattcc taaacaaaca gaataagcaa aaaaagcatt gatcccaaga
    29101 gcaggtgccg ttccaattgg ataacgggct aataccccca ttaaaataca gccgatcgca
    29161 ctggctaatg cagtcgcagt aaacactgct ccttcatcca ttccagaagc acctaataca
    29221 gttggattta caaataagat ataagccatt gagataaatg ttgtaaatcc tgccagtacc
    29281 tcacgtttta ccgtggtgtt caatttttct atttcgaagt aggaaagtat tttttccttc
    29341 atcttccatc cccctaaatc cgaacattat aaataaagtc cgttaaaatt tcgatttatt
    29401 aataaaacaa cgtgttttat catatactaa aacaaaacat aagtaaacaa aaaattcgga
    29461 ttttaagcaa aatccgaacg ttccatctga ttaaatccct ttatctcaaa gttcatgaac
    29521 attcactttg gattacaaga atttctttat tgtgttaata aagtttcttc attctagtct
    29581 caaaaatcaa tagtgtgatt tgattttacc atttcgtttc tgttcttctt tgatgaattc
    29641 ttttgtatcg acaggattca gattatttag tccgccaaaa cctgtatcga ccatttttcc
    29701 ccagcctttt gtcttctttt ttcctgattt gagattcttt gctcgaagct tctcactatg
    29761 ttttgctcgc cgatacttca aataaccttc taatcttccc attagatcca ctgtttctcc
    29821 tcctttttat tcaatcagac gactcagctt ttcctataac aaaataattg ccttcagggt
    29881 ccgaaaaatt gaaagtcaag acgccattgt tgtctgtgat ttctcctggt tcttccatgt
    29941 tctcgtagat agtttcaaat tcatccgtaa aaaatactaa agaaggcggt ggtcctaata
    30001 cctctggtga aaacttctgc acaaatactt ttgggaatac gcctaattct aattcttttg
    30061 agatgctgag tacaatactc ttaaattctt ctggcaattc gattgtttca gaaatttctg
    30121 caccaatttt ttctacaaag aatcggcaaa tcatctctac gtcattgaca taaagcatca
    30181 cacgtgtttt ttgtatcatc gtttgaccct tctttctatt tgattttctt tcctgtctat
    30241 aaactgattc cattatatca cgaaagcgca aacaccttgt gtttgatact attcaagaaa
    30301 aataaagcaa tagtttctag gattgctttc ctccacaaac tttttttaca aaaaaaaaac
    30361 ggcaatgaat gccgctcttc ttatttcgat tcttttataa ttctgcttct gattcatctt
    30421 tggttagtgt gtgtctaaca ctagttacgc caaatgtttg atcgattttt tccttcaacg
    30481 cttcgattgc ctgtgcgcca cgtgccgctt gttcagcatt gatggattca atgaccagca
    30541 ccgctgcttg tgtttcttcc gtcagcatcg ttctttgggc ttcacgccag ttgagacagc
    30601 gacagatcgc tccttgttca tcatagtaga tgatttcttc cggaagtgcc ggagcatcgg
    30661 aatctgcacc taaaggcagg aatgattcgt caccttgggc ttttcctaaa tgcagatttc
    30721 ctgcgatctt ttcgatatct tctccgccgc aaggtacgcc aaaagacaag gaaatactat
    30781 tatagatatc tacaagcgga ttgattggga aaaattcccg ttcttgattt gcacgtttca
    30841 gtaaagcttc aatagaagaa cgtgcacctt ttttagtctt gaatgtggta aatgcctgac
    30901 gccactgagc aatgacttcg ttttggctga atgtttcttc tgtaaggtaa tttcttgctt
    30961 cttttgctgc ctcattcaat aattgatgat acgtttcttc actttctttt gttacgtggt
    31021 tatcgatacc atttacggat aagatttcga tggttgcttc aggaaatact tcccagaaat
    31081 tggtatccac tattactttt ttcatctgtt cacacctctt gattagaata attgcttgat
    31141 gaatagatag tctgtttggg gatcatctcc catataaaaa acatgttcgc tgtatttgat
    31201 aaatcctttt ttacgataaa aagcgagcgc aacatcgttg tgttcccaaa caccgagcca
    31261 aatggctgtt ttttgctttt cttttgcttt ttcaatagct gtatctagta aaaatgtacc
    31321 gataccttga tgtttaaatt tttttcgtac atagattcgc tcgatttcta aaaaatgatc
    31381 agccatttcc tcagattgac tcgttcctgt atttagtttc aggtaacccg caggctcttg
    31441 atcgatactc actaggaaaa aatctgtttc tttatctttt agctcttctg taagtttttc
    31501 tagtgcataa gcttgtgtca agtaatcagc taaatgctcc ggtaattatc ttttccaaat
    31561 gtatctgtaa atgtttcgat actaatttgt tgtagtaagt ctacatctga aggcgtcatt
    31621 tttcgaattt cgtatttatt cacggcttct tcctttcaat agatcctttt attgcctttt
    31681 ttgacaaaat tccaatcttc gctaacattc ttgcatactt tatttaaaag atattccaat
    31741 tgttttgctt cttcgtcaga aagaccattt aatgcgactg cattggagta gttgttttct
    31801 cgaatgatga aaggatagat ttctgctcct tttttagtcg gatagagttt cttgattttt
    31861 ttattttctt tatcttccaa acgttcgatc atcccattgg attctaattt cttgattgct
    31921 cgggcagtcg ttgtacgatc tactttgatc atttctgcta gtttttcttg gatgattcca
    31981 ggattttcgc aaatacgtac taaataaaga tactgtcctc ttgttaaatc gacttctttg
    32041 aattcgatat tgctaataga gtctagcgct cgggcaatca ttccgatgtc tcgaaggatt
    32101 tcagccattt catcccacct ttattttgtt tgtaagacta gtttacctct ttttgttgca
    32161 aatgcaacta tttttaataa aaaaatcaag ttctttttta ataactagta aagtatattc
    32221 ttttcttctt ttacgaaaaa gctcaatcac aatagatctg gtttttgttt tctaaaataa
    32281 gtaactgttt cttcgttgtt cgcttttctg atagctttgg caactcttct atactaaaaa
    32341 atcccatgtt ggaagtttca ttattctcaa taaacttggc ctcttcaccg tggatcgcac
    32401 aagcaaaaac aagtttataa tactgaaaaa gctgtggaat atctttttgt ttattcgtat
    32461 caaaaactgc tcgcaactga gtagtttcta ctgttaatcc tgtttcttca tagacttctt
    32521 tacgcacatt ttcttcagga gacaatccta tttcagcaaa tccaccagga agtgaccatt
    32581 ctttcgtccg tttatcttcg attagtagga ccttgccatc ttttttgata taggcacgaa
    32641 catctacttt tggtgttgga tgtccttctc caatcgttgt gagcgctttt atttcttcga
    32701 aagtttcttt tcctatttca ctgatcagtt ccaatgaaat cgtccggatc tcctcaaaac
    32761 gctctttatc aaatacatct tttccataat atagaccagc ttgagaaagt gcgagtagtc
    32821 ttttgtattg agccaacttc ttgttcattc tcttcccttc ttttttagca aatgtatgac
    32881 tatgtaatca agtatagaag actggtttaa ttacggcaaa taaatataat gaaatctgca
    32941 ataaataaaa gaatgttaaa aaactcgaga acaaaagaca ttcgacttct ctggggctgt
    33001 gaataaatgg cgttcaaaag cttgcatctg cggtaataag ccccatcaaa cgaaaaatat
    33061 gacgagctat tcttgtctgg ctgttcttat taattgaggc aggacagctt tttcacaacc
    33121 tccccactaa acggtgttct cctaccaact cctcggtcac aagtcacaat attcatcaaa
    33181 catgagaagc tgtttctttg aaattgctcc ttgtgactcg gagctgaacg acaggctcac
    33241 gacctctcgg taaacggtgt tcaaaagcca atttttcgat attaagccat ttctttgtta
    33301 cttattgtca ttgatttctt tttctaattt atccaatagt ggagtgtaat ccacttgagt
    33361 atagtctgtt acatcaaatt catatagtgt tcctaactgg aatttgtcat attttcggtc
    33421 ggcgatgatc tttttgaatg cttctttcgt aatatgattg gtccctttcg ttcgatcttc
    33481 caatacgtca ccatagtgat aatgatccat gatatagctc aaatgacgat ctgattcacg
    33541 atcacgtttg taccgacgtt gccataacac ttcaaagtct gctgttaggc gaatcgtgat
    33601 cacctgatat ccgtatctat ctgctaattc ttggagtcgt gctttttgct tatcactaaa
    33661 aggatactcg gacacaatca ctttctttcc tgcttccata tacaaatcaa gtgctttgta
    33721 atagaaagga taaactttcg tcctttcttg ttccgcccgt tcttccaaat tgttaaaccc
    33781 gatggattct gcatagtatt ccttgatttc atcgagcgtg atggcataca tatctggaaa
    33841 tcgttcccgt agtttattca taagatatgt ttttcctgta ccaggactac cagccagaag
    33901 aatgaaatac tttttcatct catttatttc ctaagttaat tggactttcc aagttcataa
    33961 acagtccaat cttatgcttg acaatttctt ttactgcttt atttgctgga atcacattat
    34021 ggcgaagaga cttgttgatt tctgtttcac taaaggtttc tttagctact ttcgtccagt
    34081 tgactaaaag ctccgttcca acattgaatt tgcggatccc gttattgatc aattcaggat
    34141 aatcttcttc tttcacacct gttcctccat ggataactag ggggacatca actactgcat
    34201 cgatttcttt cagcaaggga atgtttactt ctgtttttga tttgaattgt ccatgattcg
    34261 taccgatagc aatagctaat gcatccacgc ctgtagcttc tacaaatttc actgcgtctt
    34321 ccggtcttgt atataccttg tcattttcag cgacatggat tccttcttct gtaccgccga
    34381 ttgttccaag ttctccttct acggaaacac cgtgcgcatg ggcatattct actactgctt
    34441 ttgtcttcaa gatattttct ttgaaaggta gatgggaccc gtcatacatg acagaagtat
    34501 atccgctatc gattgcttct ttgatatcat caaagtctcg ggcgtggtct aagtgtaaga
    34561 ctgcatcaac catttcttct tctgcaatag ctgtcattac attgactaac acatgcatgc
    34621 cgatatattt agcggtatcg acacttgttt gaataatgat tggcgaaccc atttcttttg
    34681 ctgcgcctag catggcagga agcatttcta ggttatgcgt attaaaagct ccaactgtaa
    34741 aattcaactc ttctgctgtt tttgttactt ctttcaatgt tgtatacatc ttttattgcc
    34801 accttatctt tttatttgtt tcctttgatc gttacttgtt ttgcaatttg tcgcatctca
    34861 tccattttgc ccatgtagta atcgatccct tgatcatcac cagcacgagc agcatctgca
    34921 cgcttctggt tatagaggct catcagtttt ttatacccat ctccgatcgt ctgtttattt
    34981 tccaagctct tttctaaata ttggatggct tgatcgttct catgcaattc agaaaattta
    35041 agtccaactt gttcataaag ttccgctaat ttatgcctat cttgggtagt ggaaatctct
    35101 tgtttcaatt gtgtaatctc tgattgaatc ttttcggtcg cttctggcga tagggctttt
    35161 tcttcttttg ccagaacttt tttcggttct ttttcttttt tcttaaaaaa tccaaacatc
    35221 attttcccta ctttcaatta aaacgctttc aaaactgggc ttaagatcca ggcatataag
    35281 agtgctgcgg ctactgtatc tacatcggtt gctgtagcat tgatgaatcc catcgaatta
    35341 aagatcgtta ctagcaaagc gggcaataat gtaataaaga aaccatgagc aataccgcca
    35401 atgatcgctc ctcggcgtcc gccaactgcg ttgccaaata tccctgctgt ccctccggca
    35461 aagaaattgg ttagcatacc aggtaaaatc atcgctaagc cgaacatggg caaagtaaac
    35521 atggcaataa tcgtaccaat cgttgtcgta atgaaaccta aaatgacggc atttgggcta
    35581 taaggaaaga aaactggaca atctaaggca gggatcgcat caggaactaa tttcatagct
    35641 atccctcgaa atgccggcac gatttcacct aacagtagac gtacaccagc tagcaatata
    35701 tacacaccca ctacgaattg gatggcttgt aaaaaggcaa acatgatata gttttgatct
    35761 cctgataatt cagaagcgaa tggttcgcct gcaaataaaa cagtgatgat atatagcggg
    35821 accatcacga ccatgacaga caaataagta tcttgcagaa attcgaaatg tgtcggcaac
    35881 ttgatatctt cgatcgactt tttattttcg cctttctcac caaaaagctt agcaacaccg
    35941 gcttcaaata agtacccgat cgtacaaaaa tgacctaaag caatatcatt tgatccagtg
    36001 attttacgaa tgatcggttg cgctactgcc ggcatagcaa tggcgaaaca agctccgata
    36061 aaaccaccga ccaagatcaa tacgatccct cgcagaccgg caaaatagcc aaagactgtc
    36121 gtcatcgttg ccatccataa aattgcttga ccagttaaaa aaatgtactt ccattttgtg
    36181 aaacgagcca aaataatatt gaatacaaaa atggctaaga acgtcagtgc aatatctcta
    36241 cctaaaccca aatcattcat tgcttgatca ttgatagatt caatagaagg gatgatccct
    36301 tgcatatgaa atccttcagt aaagattttc ccgaaataag ttaaacttcc aacgatgatg
    36361 ctcgaaccgg cactcaatac ttgaaatcct aggagtgttt ttaacgtgcc agagatgact
    36421 tgtcctgctg atttcttctg cagtagtaga cccaacatag caatcaatgc aatggtgatc
    36481 gatgcctgag tcaaaatatt ttcgatgata aaattgataa cgctcatgtt tttaccctct
    36541 tttctttttg tatttagata actcccttat cttgcagaac tggggtgatt ttttcttcaa
    36601 tttcattttt tgaaacaata tttttcaaat agatcatcgc cgttttagga tcaactgcga
    36661 acttttcgaa ctgcgaacga aaattttctg ctgtgatgat cacatctggt ttcatggaaa
    36721 ctgcggaaga aatatcacaa tggtccagtt tggcttctac tcctaat
//
LOCUS       DACGLE010000019        36009 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0534
            SAMEA3304449-rid9560453.denovo.020, whole genome shotgun sequence.
ACCESSION   DACGLE010000019 DACGLE010000000
VERSION     DACGLE010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304449
            Sequence Read Archive: ERR985064
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 36009)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36009)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702225.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702225.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 20:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,045
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 27 of 67
            Pseudo Genes (incomplete)         :: 25 of 67
            Pseudo Genes (internal stop)      :: 19 of 67
            Pseudo Genes (multiple problems)  :: 4 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36009
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304449-rid9560453.denovo.020"
                     /strain="VREN0534"
                     /db_xref="taxon:1352"
     gene            44..379
                     /locus_tag="IXT20_001093"
     CDS             44..379
                     /locus_tag="IXT20_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4859554.1"
                     /translation="MKQYLNEWKAIEGSLVTQRIKELPDCLEKDHLFQIREMLKKEQF
                     DPDQFLVVEYPTIGVYCCNHINDGKYFIIQEYGGQLTPFYTTWEMSEEGINNFPCESI
                     EDSISHTEC"
     gene            complement(636..2219)
                     /locus_tag="IXT20_001094"
     CDS             complement(636..2219)
                     /locus_tag="IXT20_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4859555.1"
                     /translation="MFILDDEIKKVKNPRIIPLLEEVRSSFYQGNYRSAIAVNYSALI
                     LDLIDKLSDLANIYEDNTSKKILEDIARLRKSDPNSPKWEMELLEKAYSSTELIDSYE
                     YEDLKYIKDQRNYSAHPVVTYNGNTWELRDITKETCQDVIRKSYEIIFLKNPILGKKV
                     TIEIIQYASRIHAMSVTPEEFHSALKNSYLNKLSEKAKENLCKTAFKFVFQLSYGNEE
                     ADRDRESTFRLLNALIFDNKEFYLNILKKEIAKYRFTAESREEINKSLGENQQITYTK
                     GFFLLTFIATHPELQHDFSDETIQNLQISINDFKPKQQVLTEIEYYYRLRSLAVLGNN
                     NEELQNHLNSLVDPILQQKMRVSSLPSSTLETIYNQYLYFGEKEMFIDFMIEHLTDAS
                     SFRLADKDMEVLPWIYNKLNQEQFNKLLYNLNGNNQFYKNSNFPPFISNLLDFYNQKF
                     QVNLRKHYSGLLYPNAVTMDQGFQLKDKELKKLYELAESKSDMYLDIIDNIINKSEDY
                     LRKEINSEKELLNYVHKIK"
     gene            2341..2925
                     /locus_tag="IXT20_001095"
     CDS             2341..2925
                     /locus_tag="IXT20_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4859556.1"
                     /translation="MDLKQSFYIWLYPDNPMSIMADIFVVSILLYLFYLIFFHNLIGY
                     MILDWKGMFDIERKPFANNQTYTVFESGDWEENEVDVIHEIGLALSGIQDADVLVSMT
                     TDTGYNAYEVFYLMYFIWRDGQEIINGRAEKTINSNTEISFCMGEGEMIVYKDGSANI
                     KTAFGDIETSIKELREYVQNPYNFQYLAGRINLV"
     gene            2936..3181
                     /locus_tag="IXT20_001096"
     CDS             2936..3181
                     /locus_tag="IXT20_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4859557.1"
                     /translation="MKKIINQPTNHLIIEGVIFVTMYFTVGNGISNTIIQKNPQYLLF
                     SLYAWIVQMVWVSILMLLFYWIVRVILLLLNKRKKDR"
     gene            3187..3909
                     /locus_tag="IXT20_001097"
     CDS             3187..3909
                     /locus_tag="IXT20_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4859558.1"
                     /translation="MKSTKLKVVLLSTAALITLTACGTTNSANNNKDTKSSSTMVTKN
                     STTSASTTMSSMTENNAEKYQKYEDINKGQKPDEVYNNFINTVKNDENYGNDPLQNKN
                     LTIVLYKPDCPDCQKAESKIMQEIKEGFSYAPTLMHDITINKPAYEYYAVAVDITDGV
                     PEWVQNIPGLADENNQYHTPTVAVVTPKKVNGTLYWDVMGMTTNTTDEMIESTTKYYM
                     DLGAYKLYQQSEPVMDDQSKVK"
     gene            4040..4783
                     /locus_tag="IXT20_001098"
     CDS             4040..4783
                     /locus_tag="IXT20_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4859559.1"
                     /translation="MKEISANEAHIPQKNQVGIYKVLHGEGIYKIQLLTGYNNGANGK
                     WLKATSIRELRSEVEKIRLGLTSEEIAKLKGNNSFVIEKEKEKHLLYRELKIEEYYEI
                     DLPEKLRIIDQRSEEKWKTEIYPSLNDYKTSGKPMYNYTGFLTYTQYKYAFRKLKGIK
                     QAEIALYEMKKVDRLKLIQDLVQNTWNQAIVELEKENGKILTNKYLIVNEIEELIGLF
                     SCEKGEVFLKSSPTYSILDSPYQFIQARR"
     gene            4789..5154
                     /locus_tag="IXT20_001099"
     CDS             4789..5154
                     /locus_tag="IXT20_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4859560.1"
                     /translation="MSSLKDDYIQAKQEYEKIQMLKSEKVELFLTQKLAEDHIANLDA
                     KTLEEYDRQFNQIHHNIILLVEQAKRRFEKAEYDLLDQGIVCLDKGLRRYYGAELKKQ
                     NVDRNALKHEILELIMQRI"
     gene            5170..5700
                     /locus_tag="IXT20_001100"
     CDS             5170..5700
                     /locus_tag="IXT20_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4859561.1"
                     /translation="MSQNISAVKQLIEEDLETTVQRLAERIHFANYQTISNWSSRNRN
                     PEEIPMKFFYRIGKEYGVDPIKIFEKTMIYYYHGTPNEVLEHLLEEAKEKSSILELSQ
                     NDKVIQVKCVDPTGKIIVGFDIERKSKIDFITQLKKINNLDILTLRPDHEQLFMSFKK
                     QLKEIQKRYNKVFPNA"
     gene            5862..6170
                     /locus_tag="IXT20_001101"
     CDS             5862..6170
                     /locus_tag="IXT20_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4859562.1"
                     /translation="MWNNRLKTGLLLIVISCAMMIGMRIQREQSYFEVSANNVIEKCY
                     YGQHYWSEEVRENIDREYVQRIVWDAYSIKDYPKSLTSRLFYSEKDNQKLSDLMMKKV
                     "
     gene            6166..6585
                     /locus_tag="IXT20_001102"
                     /pseudo
     CDS             6166..6585
                     /locus_tag="IXT20_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352869.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            6722..7522
                     /locus_tag="IXT20_001103"
     CDS             6722..7522
                     /locus_tag="IXT20_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4859563.1"
                     /translation="MSEDLRELKLRKKQYDQYFSGLLNKHIEEGLDHDFGKDEYCLYC
                     ERYNEKMSEYLQLKKEIQKLEKKVHAWKYKDSMSGYVESQNKPRKKKQAYVLKMVNGE
                     ELIVLAYSEEEVRELVETPVRKKVETINEIDDSQYNSIYIETSHGGYKKLATFLRSSK
                     VGIISKKDISAVTDSFCTLSVPKAYFQYDSKRFERLEKFCRPYTVLKTKKFNKTKRVS
                     IQLPTGQISLGEKDYLIKVGKELIIKVPEHLFKENFEIRETLVWKEEK"
     gene            7559..7741
                     /locus_tag="IXT20_001104"
     CDS             7559..7741
                     /locus_tag="IXT20_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4859564.1"
                     /translation="MAVNKILQVLIIDEFGGGEWFDVQSIDFEEEVVVIENIYGEVYS
                     YSLEGENIEDLRLIGE"
     gene            7852..8169
                     /locus_tag="IXT20_001105"
     CDS             7852..8169
                     /locus_tag="IXT20_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4859565.1"
                     /translation="MKREEYKQRLNELLEEDETLTHGSPDEILYMIDNMVIFGGYELG
                     NRSVDHNILEFDDVSWEEILDWGILAVPETKTYISDTMVPFFEELDYKRLPKNENHIL
                     GGN"
     gene            8172..8404
                     /locus_tag="IXT20_001106"
                     /pseudo
     CDS             8172..8404
                     /locus_tag="IXT20_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321015.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(8519..8998)
                     /locus_tag="IXT20_001107"
     CDS             complement(8519..8998)
                     /locus_tag="IXT20_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4859566.1"
                     /translation="MTDKTSDLPKLAPKTSRVKSKKDGKTKNAVKKKSVKETSSLLLD
                     IPMSSFDDNTDETSVKIATHVGMNNQNDSFYNLLNSLRELEDKKNQVIASKAPDLTTD
                     LDAEIIQIKQDERSVKSLLKTYFDGFDKELAPYSQAILTKPIQAPHISISLQKKSDD"
     gene            9118..10455
                     /locus_tag="IXT20_001108"
     CDS             9118..10455
                     /locus_tag="IXT20_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HAQ4859567.1"
                     /translation="MLMVQFTGIAMSVITINPQVIHADGIATYDAQFNCTTEGSGSGT
                     DSDGNDTGSGAVTGDVAKNIEDIYKYMNGEYGLSGASIAGILGNWKQESGIDPRAVEG
                     YYGDGQREVDFIKQFTDGLMGIGYGQWTAGRHTALVDWSKKNNGGSWWTTEAQLDFMF
                     KGDGGFVGILKDYALNASDDVVENAVTFHKNWEISADSESAVIAVRGENAKEIWKYMK
                     SKGMDGAKDESKINKISGGSSTGDGSNNSSSSASGTVEDACATTDDANSGSGSIGKEG
                     KINGKKGQVIANMTYEEALKKYGKAITLPKFKEPDWSTSPFNGIYQGQCTELTWAYMS
                     QLYDGNQPTTGNGGYVWESYKSAGAKITNKPIVGYGFSASDGWLWAVGDAGHTGVVIA
                     VFEDGSFLCANFNVPPTQAPTRGVSVTLIDGVDGSDNVHFFSGVGKAKFEIEK"
     gene            10478..11005
                     /locus_tag="IXT20_001109"
     CDS             10478..11005
                     /locus_tag="IXT20_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4859568.1"
                     /translation="MNSKVKDTIIISLLSGMLLALLVGILFFNKQNQDIEAEKHSVHI
                     EDKLTFFKEVKPNEVDGLRKDLENDIQEMLNGSVQNKFADQSGEEFLRKLFIVTGKPP
                     ITDKSSEKEKIEYYKPFTFKLTNFGIKTSSSGLYEALTDFEVQYNGKKVYDTTLTITL
                     NNDKKVTGGAFYAKS"
     gene            10992..11555
                     /locus_tag="IXT20_001110"
     CDS             10992..11555
                     /locus_tag="IXT20_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4859569.1"
                     /translation="MQNHEITTAKKAIGILSIIVILCCVVLFQKKNAYDNNVKELEDL
                     KSKQIEVVKNNQKVKAMQEAQYKELGNAEIKEQSRKFFNLFYNWNSWEKYTKNMKEIQ
                     KEFPQIVKSSPVDISGTMVGTGNSPISSYTADYYTTNQKGKMVELITQTRNTPTTATT
                     TIWRGVTSIGENQLFYLEDLDSYVRAE"
     gene            11977..12714
                     /locus_tag="IXT20_001111"
     CDS             11977..12714
                     /locus_tag="IXT20_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4859570.1"
                     /translation="MADHKLEKLLEETWNPKEFSEFFMENFETDLAVIVKDALREQGY
                     PETANYININFTLYTENKGTWDFWATLANKELSDKSDTGIRNFFESNRDDYMYANNQN
                     KLNFRVEFDETPEEFIERQPPKENVAKVLEDRWNSDEIVSTISELGGQYEPLVEAVRE
                     ELRLNKFPDVQNIDVSQIEINVKITNKLDYGSWADIALEKYIYSTLKEFIENRMDIMY
                     LQHPQYLNFGVEIATPLEEWKMEQGLD"
     gene            complement(12731..13147)
                     /locus_tag="IXT20_001112"
     CDS             complement(12731..13147)
                     /locus_tag="IXT20_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4859571.1"
                     /translation="MNKKRKLSVLITLVSILTILSVILSLKIFNQINTSSNAKDGIVV
                     ENTSNSKETGTTTKKDKSAEITTKNYLIKTVYFDERKTAILKDEEGSELKLPITVSDK
                     FNGTFSLQTAEGSPDNLKVGQTIILGKNDELYSFVK"
     gene            13540..14787
                     /locus_tag="IXT20_001113"
     CDS             13540..14787
                     /locus_tag="IXT20_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4859572.1"
                     /translation="MNMKRNSLGMVLLASAVLAGLSVPVTSHADEWTANTPDQIQITT
                     GQSSYAFKKGDTIWSTVEKLKSLGYKTTVAEFAELNNVDLAKGEQRTIPIGRTFNISK
                     DTLTVKEADGEVVSQTVQKDKPDPIENTEQENKKSETTKESEPTANTSDKTETSASSV
                     DGASSSNTNNSTSEKEASQGTSESSTTEADTGTSTNNSQSTSNDANESTSTSSSDQSQ
                     TGSSDETDSSTSPSKPEQPTNVVLGVPFISQGNTMLCEGTSLLEALHYKGVATNQDLM
                     TFVKSMPLSPDNNPNNGFSGEWRHNVDGTYQGMNPAPVVSWGVANGGNVVDISGSSVQ
                     GLKDEIVNGNPVVAWVTYAFEPAQHKPMFWGDAIWNRHVVAVDGFKDGFYHVVDPVFG
                     ASWINAGSFETAYNVSQLAVSVR"
     gene            15055..17445
                     /locus_tag="IXT20_001114"
     CDS             15055..17445
                     /locus_tag="IXT20_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-Gln thioester bond-forming surface protein"
                     /protein_id="HAQ4859573.1"
                     /translation="MKHEKTIILKSKIIYCFATLVMMLSTLIMPFSAIRAEADVTKPT
                     SAKFIETGVKTDGYIRVNMPNHPNEWMISSQFKDSHGNIGYCMDSELPSPTGSGAGSL
                     KYKGAGSDEFYRMFKGGFPSKTAKELGAGNDTEAWYATQLVSWVLAGNFKVSQIVWSH
                     PNHTAAETARVKKAFEKIYDYAKNGKDTPNTEFSITASKTADEGKYHTFTYKTASNKT
                     GNAKLTFTSAKPAGMKIYDADGKEITNNTVKLNSSFTIKVPVTTPSGTLSFKGTANVS
                     TTNPFTFDGRGVYQDAVVMITTSETKDSKSLSAKWTRAVGQVTVKKVDQDGNALKGAE
                     FTLTDNNNAKSVKTTGDDGLATFDLQQGLTYSLEETKVPEGYQGSFKKDGITVNTDGQ
                     EFSYTATNTIKKGKVKVKKVDQDGKPLANAEFTLTDDSSQTQAEKTDENGLASFDIVA
                     NKTYSLEETGVPTGYTGVFKQEGITVKDDGQTFEYTATNTLKKGLVKVHKVDQDGKPL
                     ANAEFTLTDEKGATQVEKTDENGLASFNIVANKTYSLEETGVPVGYTGAFKQEGITVK
                     DDGQTFEYTATNNLKKGVVKVHKVDQDGKPLADAEFTLTDEKGVKEVKKTDATGLATF
                     NIIANKTYNLEETKVPTGYTGAFKQEGITVKDDGQVFEYTAKNTLKKGVIKVHKVDDQ
                     GKALANAEFTLTDDKGVKVVQKTDSNGLATFNIVANKTYKIEETQNPNGYTGKWSKEG
                     ITVADDGQTFEYTVINHLVPETVRTGGIGTSAFLLVFLIAGLSMVLLYYREQHKAK"
     gene            17494..19329
                     /locus_tag="IXT20_001115"
     CDS             17494..19329
                     /locus_tag="IXT20_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HAQ4859574.1"
                     /translation="MKSKKIFSNLMLFGTLLGVGLGASSISSSLGLGGQVAYAQQNYN
                     YQGTGTIHIHKVTNTNGDGNPVSDIDNKGKEIALPNGVKPLEGVEFSYVKLTKDEAKS
                     LGWTDSDTTKGQHIDDAKARLLLNKKYSEVSRMKTDSQGNIDIPIQVNTDDLTDNLYL
                     IVETGTVKGVVEEAYPMIVNVPELNGSDDQYFNYDVHVYPKNYFQLNSAELDKKDEDG
                     KALAGTVFELRKDDGSDQVVGTYKTDSTGKLTVDGLELGDYYFLETDLGTDANKTTYL
                     LDGKKFKFSVKRGETAKVVLDVTNYKRPVITKVIDKETVDTGEDVQYTLKPSIPGNIG
                     NYEKYVVKDVLGAELTYDSSEVIEGMTLDTDYSIERDGQTTNYVFTESGMDKLQKQFD
                     AGDRFFTILMNAHTNSKAKMDTPLKNDVSTIYNNGYEPEDETPVDHAYTIIGDHQFIK
                     TDGDTSKALSGAKFVVKNKIDPNKVTYLKQDPTTKSVTWIADKSKATEFVSGSDGKFQ
                     IEGLQYTYDVDFDKQGNPTITDKKINDYALEEVVAPSGYTPTRNDVPFVVSNDDASSE
                     VTDIKNLKTPETVRTGGIGTVVFLVVAGVAGTGLYVVYKREQHKA"
     gene            19404..20261
                     /locus_tag="IXT20_001116"
     CDS             19404..20261
                     /locus_tag="IXT20_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAQ4859575.1"
                     /translation="MKKKNIKLIILKIGMLLCLGVGLYPVITQAYTYYQTQQTLNSYK
                     EKYDEASEKENEEVFKRYSEVNRKRKDTEQTSVTIPEDNNNIPSNGNLQKDKLGELVG
                     EILIPKINVDLPIYDGVNDFQLSAGAGILTGTDYPTGGIGNHSVISAHSGIPNKKYFT
                     DLELLKLGDKFFIDISGKKLAYQVDRIKTILPTEFEDLLPVAGKDYVTLMTCTPVGDN
                     SHRLLVRGVSIPYQKSIVEKEKKKEDLKVKAQMNWYPIVVVGVLLIIAVIVIIKKIVA
                     KRRGMKNEE"
     gene            20251..21360
                     /locus_tag="IXT20_001117"
     CDS             20251..21360
                     /locus_tag="IXT20_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAQ4859576.1"
                     /translation="MKNKILLFLTLICTLVLTIYPANQLYHQFKTKENVAEIQEKIEK
                     KQTPSKEEVAKKVEQIKSNSGNQGLDNTQKTDKGVDNTQETDGGSFDIREAEGKVNPY
                     TGDVFSKNQTKDFEPSTYSGAVGYVYIPKINVLELLFLGASQEHLYRGVAQMSATSLP
                     LSGKGHQTVIAGHRGGYTSPQFLYVDQLVAGDHIYIDYLGEKAVYTVFRTDVIRNYGQ
                     NSTLTDIPDLDMLTLYTCHPYPQNYDRLLVRSLRDEKAKYITPQVTVGNTAYADAIDS
                     ESVSDKTETVTSSNDLFEDVDRANKELDGNNDLNIPEQGSVEMQDRTFAEKASSIFTG
                     ITNKHQKMLLVIWTVIGALASLKVLILLIKEFRKA"
     gene            21699..22499
                     /locus_tag="IXT20_001118"
     CDS             21699..22499
                     /locus_tag="IXT20_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4859577.1"
                     /translation="MVGVSKTALDSLAKLFSSRFDGAEFVEYLSQNESGKTLIETMKK
                     DYLNENDFPTWTNIEDATWDVVCTIYSPWVTIADFVLDKIGGDTYPDAKSLATSDFKT
                     FYGYLIGLIGNGIDLKVTPKQTPEEIEKEKGEVQMAYANDREKVKLLEGKLSEEELGK
                     MVQEINEDSNYFPELKVYQNDEDFFNEMFSNSPWEAVSKTNLGEWNFQDDLVAFSNDG
                     YLRSYSESEYRAELKTYETDILMSYLNLLESGQGELRDKVEELLKEEL"
     gene            22587..23393
                     /locus_tag="IXT20_001119"
     CDS             22587..23393
                     /locus_tag="IXT20_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4859578.1"
                     /translation="MRGDQTMSATTDMALEAVIKDNTGNVENVEDFSDIAINLSAWIS
                     AVGEENIEFEIKNMDDLKAKFPFIASKLSQVAEDKSWNELVSFSEKIYDITQEDECVS
                     WEMNNQYKKIDAFVKSRIEGTAFEFEGIDTADNWLNKESFIKSIADDLEKQWSKEDYA
                     EYLKTERDTEVKEKLTEVCLDEHIDPNIVKQEDYEVVIDKVPTDFEEVVKYIVEEALS
                     QYSSDEEVDEETVRTRVWQLVFDDKDYKYLSDQNSVNWSVRLLIDQNDIE"
     gene            complement(23415..24842)
                     /locus_tag="IXT20_001120"
     CDS             complement(23415..24842)
                     /locus_tag="IXT20_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182 family transposase"
                     /protein_id="HAQ4859579.1"
                     /translation="MMRKQSIEGRNQFAMLTIDDLVPKDHLVRKIDAAIQFDFIYPIV
                     ESTYSTYGRPSIDPVVLIKLVFIQYLFGIRSMRQTIKDVETNVAYRWFLGYSFEDPIP
                     HFSTFGKNYVRRFRETTLFEDIFSHILEQAVKAGFVTEDNLYIDSTHIKANANKHKFT
                     KEMTYGQAKAYQDELEDEINKERIAVGKRPFTWDTESELSLQKISHSDPESGYYVKGE
                     REKQFAYSAHTSCEDNGFILSTLITPGNVHDSQVAIDLVKQSKRAFPNIKRVVADAGY
                     KTPKFVHFLSHLKLYPILPYTAPHGVKGMFRKKDFVYDAYFDCYLCPNNQPLLFSTIT
                     RDGYRTYKSNPKFCVSCPLLENCTLSTKHQKIVTRHIWADLMDELEHQRHTALNREIY
                     KKRKQTIERIFADAKEKHGMRWTKYRGLEKVATHTMLTFAAMNLKKLATWLWKTKKPM
                     AFYFKIMQRLTKRSGLITNSFLSTI"
     gene            25163..26032
                     /locus_tag="IXT20_001121"
     CDS             25163..26032
                     /locus_tag="IXT20_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease"
                     /protein_id="HAQ4859580.1"
                     /translation="MKKKKFSRSLLGILVLVAVGLSGKGYFQNNSTQEHDFQIKSSVQ
                     VGNKADSESKEFLGIRYDPNKYPNNIVNINDGKYTLTKEDHEALSEKGSQTFWVNYAD
                     LDQLGRAGDVEALITYDAVNSHSSKNVERPSFKSSVHIAGEYKDGVFDQERDTWVGKD
                     SNNEITQLEGYKGYLYNKSHSLAWSLGGDMEVHNLTLGTRAQNVGKNDQNGGMAYSET
                     IVRDAIYKNKNEKVLYKVDPIYKGNELVPRGSHVEAFSVNDNGKTVNLNVYVFNNQKG
                     ININYQNGTWVRD"
     gene            26052..26399
                     /locus_tag="IXT20_001122"
     CDS             26052..26399
                     /locus_tag="IXT20_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4859581.1"
                     /translation="METISINGLDKSVANRIKKGNGYYLLKKGKYSVARELETMSIVY
                     VAKNHFDVEQAIFDPDSEIGKCVELSEKTVSTKGAMIIEREMLDEIRVIIPKDTQMCE
                     DSISDDEPKSERK"
     gene            26412..27674
                     /locus_tag="IXT20_001123"
     CDS             26412..27674
                     /locus_tag="IXT20_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4859582.1"
                     /translation="MSEKSIELKEYKRSDGEKYYPVASFSKSVYLVGRALVVGAIGIY
                     ILHWVTKVVQLLVDTLVSILVSLNDVNGTDDLGLKEMFKELLNAFFPNYLSYSYNVQS
                     FFAKLHLVDISILVLEAGTVFFVGYLVTLMFRHHYGEQRPFFDDMESRRLKREALEAM
                     DVKYDPNKIIGDETNNKKRKRNKTQLESDVLSTLKNIKISVHTRRDLSVNEDIREYIV
                     KIPQPKGKKVRETVLRQIQDLGSILTRATSGRAKFGEKEDEVSGDYFIFRASVNIERE
                     RKERVSTKNKETERATSSIAVSNSQFSFPINLFADNSKNIQNQTEKARKYAETQKQSL
                     ELYLSSADVPASLTDMHVGNTSIEYLFTLSRTRKIQLSSMKEEIETYLHISGVIVRIN
                     AGRIQITLPLGENDRIPIDVPSMINSVF"
     gene            27754..29130
                     /locus_tag="IXT20_001124"
     CDS             27754..29130
                     /locus_tag="IXT20_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsK"
                     /protein_id="HAQ4859583.1"
                     /translation="MAMKLLEKVKKRAEKAESILLDKEPNIKLNGKFSVNMLNTKAKD
                     PTDAIFGIKPDGTIAHFCISEMPHMLVAGTTGSGKSVLLNEILVTVMCHSTPDELKIG
                     IVDPKRVEFGRYKKLPFMLADPITDMDEAYDFFEYLVILMHERYKLMEKAGLQNITLY
                     NEYAEKNGLDRFPYVILLVDEYSQLVGTHKEVEGLIVQLGQMARAAGIHVILATQSPR
                     STVVTGIIKANFPSRVCLMVASDLESRIVLDEGGGESLSPKGDMLIKLVDGSMVRAQG
                     AYISNKEIETIFNHLRNTMPEPEYVDYRAIVAETRGEGGEDEDFGGIGKAPVRKSAKR
                     NLNTPLANPSSKEPSIEKKSAFAIKLEKNKEEKVEQGAKTLEKLQAEKLEKVLRALGK
                     ADPEPQTSNNTNNKQPIASKTKPKANDPKGSENVVITTDKSEKKQINGSTSPIMQKML
                     KAKKQVHK"
     gene            29127..29756
                     /locus_tag="IXT20_001125"
     CDS             29127..29756
                     /locus_tag="IXT20_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4859584.1"
                     /translation="MIIVSSFLRWLTPYKYTDEVVVKRAKKRDKKAVLTPKQMLDHGI
                     LGVKVLEVNKPFESNSAREKLLGAEADVAYFYHDKSKHQIAIKFNPKSAWRYQARDDL
                     KRRKLRPMLYPEGKEAFDRTHIVPIGFHGTESDNRLLVGWNSNQNRVQINKFEKKVRK
                     FNDRKTILWFADICKQPDGTAKWEAVIFDENGKKVMSETWHDKAKFVWQ"
     gene            30574..30627
                     /locus_tag="IXT20_001126"
                     /pseudo
     CDS             30574..30627
                     /locus_tag="IXT20_001126"
                     /inference="COORDINATES: protein motif:HMM:NF019230.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            30631..31230
                     /locus_tag="IXT20_001127"
     CDS             30631..31230
                     /locus_tag="IXT20_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HAQ4859585.1"
                     /translation="MREKNKNSSRYLVATVVFVCVLIFLFDFLQIGKSIIGNTLSAKE
                     MVALGGLYEGHLFPNIFISIVSHINLYHLISNLLFLVVLEEALNDQYEWLTLLIVLII
                     SGFLGSLVPFLLHSNIVSMGLSGGIFGWIALFAIAYIRFDSKKHLLLSVFVLLIIGIL
                     LVSTFESGEANVLSHVIGFVCGMILGFILKNVSLIKQKN"
     gene            31246..31722
                     /locus_tag="IXT20_001128"
     CDS             31246..31722
                     /locus_tag="IXT20_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4859586.1"
                     /translation="MPKRKEKKIPVFLSVSEMLSLQILLRKKIETIDEEVRNKRAMYE
                     QREALRERIHYFDDIHTKIYEARTNHEDESKLGLAQQRILEWLNTNTDQEPLRVISNL
                     FEFGTKHWFNAVEKDADLQEAFDTLSKDSKSMRDLLITYLNEHEFEQTKTKKKNKE"
     gene            31739..32641
                     /locus_tag="IXT20_001129"
     CDS             31739..32641
                     /locus_tag="IXT20_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4859587.1"
                     /translation="MLLTLLGYLCLILAFFGAIAIIIGQFQTNNPIMRNIGIGIVIVT
                     FIVFNATKIFGSNDKQFGISGVNNSYAPDSLGRVVIDGTFENVTSYKNVSLTVKEDGK
                     KASDDVLATIKPSQSQFEVFYTVKENSPVHKLTLVFNVDGEEYYKDVKINKTSTKKII
                     ESAKKEVATEESTTESSSTVEAELPDKIDFDPEKVKSFESVSGSMADRFNTEVFKAIK
                     TVKLDTEKQMINVTLFNSVESMDQDKKVTVARELGKRVIGLYRGTAKPDSDGADLPVH
                     VVYEDGKVFGDSTANEALTIKLAK"
     gene            32714..32905
                     /locus_tag="IXT20_001130"
     CDS             32714..32905
                     /locus_tag="IXT20_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4859588.1"
                     /translation="MVVNKEKVRKYVTLKIASVKYLEKRAKEEERARGKKVTVSELLE
                     ELIENDQTIRRAFTNRSIR"
     gene            33069..33656
                     /locus_tag="IXT20_001131"
     CDS             33069..33656
                     /locus_tag="IXT20_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4859589.1"
                     /translation="MENNNDIRHFSIGERVTTVSELSKDYRDKEGTIISLNKNRRGVW
                     QYVVDLGDGVTVECKDSQLIRNNGSHSIEKAILVYPKVSSKIMNDWLLGKFGEYNKKE
                     IAEVIGKSYPTVLGYLKKITSKEAKIEDVLTVRELQSLIEYRNKQITNPEYLRKEDGL
                     LKQLEQVNKIRKMQKVAPLSLDTFKKIYLKEIKKD"
     gene            33656..34555
                     /locus_tag="IXT20_001132"
     CDS             33656..34555
                     /locus_tag="IXT20_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HAQ4859590.1"
                     /translation="MWTVTLPFQPVSAPRPSARRNAADETNDVAEKRISTYYDKKYLD
                     YLKKIKSFIEDQDLLDDEFYSIVNDPMGAIMEVDFYYQIPKSYKKVYNIMKTTAPDLD
                     NLVKSAMDGIFNISAIRDSHITGLIAFKYNVANEGKTVVRIKRYSEFEWDTSEGLNGD
                     IWEATFDFKPVATPRPKSKFRGNGIITYYPKEYNDYLENIKNTLKEKDLVNDQLKKVM
                     NAPMGVIAQIDYFYQVRKDKKKLDSLMRTSQPDIDNLVKGTLDGIFNKEIGIKDSRVV
                     GLIAFKYNTFEKSKTNVRLQEMG"
     gene            34555..35469
                     /locus_tag="IXT20_001133"
     CDS             34555..35469
                     /locus_tag="IXT20_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4859591.1"
                     /translation="MNNNEKILHVLDSFEIIQEELKKYRDVLEQRYDFVNQQKSNHMD
                     FILNMNDLKKKLVERKEQEKLIKAYFELGEKEVKNAMELNEERRVLDQLLEQLLVMFQ
                     KGRIDEDLIEEGLRKYPSNSGIGIVLKAIDEEEIEAFIPPEDFESAMEYIKYYSQGIT
                     AFREFDPEDVIHDLNNLKEWCESYGVDDSGLDYLISIMEIEEEMPDKPDPTDILELIH
                     EARNPIAYISRGYTVLEYYKPYISAMNHLRRVLREKREYRSVLNATNRLEKAVSELDA
                     YYREHYLQAGGMPRNTKANISRYIKKAE"
     gene            35521..35736
                     /locus_tag="IXT20_001134"
     CDS             35521..35736
                     /locus_tag="IXT20_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4859592.1"
                     /translation="MKRDVFELYVLERLEDGMMAQEIIDEIDDMISRFGQLELNDGNK
                     NSVVLKQFWLNNFSYKDFIKEKANTLY"
BASE COUNT        13408 a         4992 c         7061 g        10548 t
ORIGIN      
        1 aatttcctag gaaaaatagt taacataaaa ggaatgaatt gttatgaaac aatatttaaa
       61 tgaatggaaa gcgatagagg ggtcattagt aacgcaaaga ataaaggaat tacctgattg
      121 cttagagaag gatcatttgt ttcaaatacg agaaatgttg aaaaaagaac agtttgatcc
      181 agaccaattt ttagtagtcg agtatccaac tattggagtg tactgttgta accatatcaa
      241 tgatggaaaa tattttatta ttcaggagta tggaggacag ttaactcctt tctatactac
      301 atgggaaatg agtgaagaag gaatcaataa ttttccatgt gaaagcatcg aagattcaat
      361 ttcacacacc gaatgttaat tgattataac tataaaaaag gattgaacat tatgttcaat
      421 ccttttttca atgaaatgtt ttccgccatt tcattgtttg gtgcgatttc tattttatta
      481 tatcaaaata aagagagatt ctaaagaaag aagagggaaa tatataaatc aagcgacagt
      541 atatttatta ttaaatgcta ttgtgagaat ataactaatt agtttaattt gaaagtaaat
      601 atacgctata aaagtgcatc ttgtgttata aaaaattatt ttattttgtg aacataattt
      661 aacaattctt tctcggaatt gatttctttt cttaaatagt cttcagactt attaataata
      721 ttatctatta tgtctagata catatcactt ttcgattcag ctaattcata cagttttttt
      781 aattctttat cctttaattg aaaaccctga tccatagtca ctgcatttgg atatagaagc
      841 ccactataat gtttacgtaa attcacttgg aatttctgat tataaaagtc taacagatta
      901 gaaataaaag gcggaaaatt actgtttttg taaaattgat tatttccatt taggttgtat
      961 agtaatttgt taaattgctc ctgatttaat ttattgtaaa tccatggtaa tacttccata
     1021 tccttatctg ctaacctgaa tgaactagca tcggtaagat gttcgatcat aaaatctatg
     1081 aacatctctt tttcaccaaa atataaatat tgattatata ttgtttctaa tgttgaagat
     1141 ggtagcgaag aaactctcat tttttgctgg agtataggat caactagact atttaagtga
     1201 ttttgtaatt cttcattgtt gtttcctaga actgcaaggg aacgtaaacg gtagtaatat
     1261 tctatttctg ttagtacttg ttgtttcggt ttaaaatcat ttattgatat ttgtaaattt
     1321 tgaattgtct cgtctgaaaa atcatgttgt aattctggat gggtagctat aaaagtaagt
     1381 aaaaagaacc cttttgtata cgtgatctgt tgattttcac caagtgattt attaatttcc
     1441 tctcttgatt cagctgtgaa cctatacttt gcaatttctt tttttagaat atttaaatag
     1501 aattctttat tatcaaaaat tagggcatta agtagtctga aagtactttc cctatctcta
     1561 tctgcttcct catttccata agataattga aaaacaaact taaaggctgt cttacatagg
     1621 ttttctttgg ctttttcgct tagtttattt agatagctat tttttagtgc agagtgaaat
     1681 tcttcaggtg taacagacat agcatgtatg cgacttgcat attgtataat ttcgatcgtt
     1741 actttttttc ctaatattgg attttttagg aatataattt cataggactt ccgtataaca
     1801 tcctgacaag tttcttttgt tatatctctt aattcccaag tattaccgtt gtaagtgact
     1861 acaggatgag ctgaataatt tctttgatcc ttaatatatt ttaaatcctc atattcatag
     1921 ctatcaatta gctcagttga actataagct ttttctagta gttccatttc ccattttgga
     1981 gagtttggat cactttttct tagtctagct atatcttcta aaattttctt agaagtatta
     2041 tcctcgtaaa tatttgctaa atcagataat ttatcaatta aatctaaaat aagcgcactg
     2101 taattgacag ctattgcaga acgataattt ccctgataaa aactacttct aacttcttct
     2161 aataatggaa taattctagg atttttaact ttttttattt catcatctaa tataaacata
     2221 taaatctcct ttttttattt gtatgacaag tttaccataa aaaaaaatta aaaagcgaga
     2281 catatttatg ttgttgtgtt atcataataa cataaatatg tagagtgaga tgatgaaaaa
     2341 atggatttga aacaatcgtt ttacatctgg ttatatcctg ataatcctat gtcaataatg
     2401 gctgatattt ttgtagttag catcttattg tatttatttt atctaatttt ctttcacaat
     2461 ttaatcgggt acatgattct tgattggaaa ggaatgttcg atatagaaag aaaaccattt
     2521 gctaacaatc aaacctatac cgtcttcgaa agtggagatt gggaagaaaa tgaagttgat
     2581 gtgatccatg aaattggatt agctttatca ggaatacaag atgctgatgt tttggtatca
     2641 atgacaacgg atactggata caacgcttat gaggtatttt atcttatgta ttttatatgg
     2701 cgtgatggtc aagaaattat caatggtcgt gctgaaaaaa caatcaattc taatactgaa
     2761 atttcttttt gtatgggtga aggggaaatg attgtatata aagatgggtc agcaaacatt
     2821 aaaacagcct ttggtgatat tgaaacaagt attaaggaac ttagggaata tgtgcagaac
     2881 ccatataatt ttcaatattt agcaggaaga ataaatttag tttaggagta aaaatatgaa
     2941 aaaaattata aatcaaccaa caaatcactt gataattgaa ggtgttattt tcgtaacaat
     3001 gtattttact gtaggaaatg gcatctcaaa tacaatcatt caaaaaaatc cacaatacct
     3061 attatttagt ctttatgctt ggattgtaca aatggtttgg gtttccatat tgatgctgtt
     3121 gttttattgg atagtacggg ttattctttt attgttaaac aaaagaaaaa aggataggtg
     3181 aataaaatga aatcaactaa attgaaggtt gtattgttat cgacagcagc attaataacc
     3241 cttacagcat gtggtacaac taatagtgct aataataaca aagacacaaa gtctagtagt
     3301 acgatggtta ctaaaaacag cactacatcg gcatcaacaa caatgtcttc aatgactgaa
     3361 aataatgctg aaaagtatca aaaatacgaa gacattaata aaggtcaaaa gccggatgaa
     3421 gtttataaca actttattaa tacagtaaaa aatgatgaaa attatgggaa tgatccattg
     3481 caaaataaaa atctgactat cgttctttac aaacctgatt gtccagattg tcaaaaagca
     3541 gaatcaaaaa tcatgcaaga aataaaagaa ggatttagtt atgctccaac attaatgcat
     3601 gatattacca taaataaacc agcttacgag tattacgcag ttgctgtaga tattactgat
     3661 ggtgtacctg agtgggtaca aaacattcct ggtctggctg atgagaacaa tcaatatcat
     3721 acgccaacag ttgcagtagt aacaccaaag aaagttaacg gcacactata ctgggatgta
     3781 atgggaatga caacaaatac aactgatgaa atgattgaaa gtacaactaa atattatatg
     3841 gatttgggag catataaatt atatcaacaa tcagagcctg ttatggacga tcaatccaaa
     3901 gttaaataga tctttagggc gtaagcccta tttttttaaa attaagtgat aacaaataaa
     3961 tgatttctga aacaaacgtt cttactcaac tactcgaaac acacaagcta attaactaga
     4021 ttataaagga gaatgcgtaa tgaaagaaat cagtgcaaat gaagctcata ttccgcaaaa
     4081 gaatcaggtt ggtatatata aggtgcttca tggtgaagga atttataaaa tacagttact
     4141 taccggttac aataatggtg caaatgggaa gtggttaaag gcaacaagta ttcgtgaact
     4201 tagaagtgaa gtagaaaaaa ttaggcttgg tctcacaagt gaagaaatag ctaaactgaa
     4261 gggaaataat tcttttgtaa ttgaaaaaga aaaggaaaaa catctacttt atcgtgagct
     4321 gaaaattgaa gaatactatg aaattgattt acctgaaaaa ttaagaatta ttgatcaacg
     4381 ttcagaagaa aaatggaaaa ctgaaatata tccaagctta aatgactaca agacatcagg
     4441 aaagcccatg tataactaca caggcttttt aacctacaca caatacaaat atgcctttcg
     4501 caaattaaaa ggcattaaac aagctgaaat tgccctgtat gagatgaaaa aagtggatag
     4561 attgaaattg attcaagatt tagtgcaaaa tacatggaat caagcgattg tcgagctaga
     4621 aaaagaaaat gggaaaattt taactaataa atatctgatc gtaaatgaaa ttgaagaatt
     4681 aattggttta ttttcatgtg aaaaaggtga agtcttcttg aagtcatcac caacctattc
     4741 aattttggac agtccgtatc agtttataca ggcaaggaga tgagttagat gtctagcttg
     4801 aaagatgact atatccaagc aaaacaagag tatgaaaaaa tacagatgtt gaaatctgag
     4861 aaagtcgaat tatttttaac acaaaaatta gcagaggatc atattgctaa cttagatgct
     4921 aaaactttag aggaatatga tcggcagttt aatcagatac atcacaatat cattttgctt
     4981 gttgagcagg caaagcgaag gtttgaaaaa gcggaatatg atctattaga tcaaggaatt
     5041 gtatgcttgg ataagggatt gcgaagatat tatggtgcag agttaaaaaa gcaaaatgta
     5101 gatagaaatg cactaaaaca tgaaatctta gaattgataa tgcaacgaat ttgaagggat
     5161 agaaagcaaa tgtcacaaaa cattagtgct gtaaaacaat taatagaaga agatttagag
     5221 accacagttc agagattagc ggaacgaatc cattttgcaa attatcaaac catctctaac
     5281 tggtcaagca ggaacagaaa tccggaagaa attccaatga aattttttta tcgaatcgga
     5341 aaagaatatg gtgttgatcc gataaaaatt ttcgagaaga ccatgattta ttactatcat
     5401 ggaacaccta acgaagtgtt agaacatctt ttggaagagg caaaagaaaa atcatcaata
     5461 ttagagctat cgcaaaatga caaagttatt caagtgaaat gcgttgatcc aacaggaaag
     5521 attattgtcg gttttgatat tgaaagaaaa tccaaaatag attttattac acaattaaaa
     5581 aagataaata atttagatat tctaacgctt agaccagatc atgaacagtt gtttatgagt
     5641 tttaaaaaac aattgaaaga aatacaaaaa agatataaca aggtgtttcc taatgcgtga
     5701 aaagctcaaa gaaatcagag ttttttggat aattaacaaa gaatgtatca tcttatatac
     5761 tcttggattt cttagcatga tcttgttttg gtggttatat ttaaaacagt aattggagag
     5821 ggtcaagagg atttttatca agataaggat gtggaaactg catgtggaat aatagattaa
     5881 aaacaggatt gttattaata gttatttcct gcgctatgat gattggtatg aggatacaaa
     5941 gggaacaatc ctattttgaa gtttctgcta acaacgtaat agaaaaatgc tattacggtc
     6001 aacattattg gagtgaggaa gtcagagaaa atatagatcg tgaatatgtt caaagaattg
     6061 tttgggatgc atattctatc aaagattatc ctaaaagttt aacgagtaga ttattttaca
     6121 gtgaaaaaga taatcaaaaa ctctcagatt tgatgatgaa aaaagtatga ctattattac
     6181 gaaagagaaa cagagtgcta tattcgaggg gaaaaagcct acaacaaaag tgactattgg
     6241 ggtgacaagg cgaagcgatt aactgcaaat tttagagtgt atgaatcaga gtttataaac
     6301 ttgacctttc ttaatagtgt ttttattgat tatgtaatta ctacgaaacg cattggaagt
     6361 tattcaagta cctacgcatc cttagttcca atgttaaact cgatgagccg tttcttaaaa
     6421 gaaagagaaa agaaagagct acagtatatc aaagaaatca tgccggattt tgaaatgcac
     6481 gaatggcaaa ttgctttatc agattggaaa ttggcgaata aggttcgtag aataacacct
     6541 tttcaagcca agcgcttttt gagagctatg aaatttgtgc aataaaaaaa gccaccagac
     6601 aagttgatgg agactaacac aattaccaac agttgtaatt gtgttagctt acaaattaat
     6661 tatggtgttt agtcaatccc aaattgtgaa atggcaccaa taattggaaa ggaaaaatga
     6721 aatgtctgaa gatttacgag agctgaagtt acgaaagaag cagtatgatc agtatttttc
     6781 cggtttgttg aataaacata ttgaagaagg acttgatcat gattttggaa aagatgaata
     6841 ttgtttatat tgcgaacgtt acaatgaaaa aatgtctgaa tatctacagc tgaaaaaaga
     6901 gatacagaaa ttagagaaaa aagtacatgc ttggaagtat aaagattcca tgtctggtta
     6961 tgttgaatct caaaataagc caagaaaaaa gaagcaagcg tatgttctaa agatggtaaa
     7021 tggggaagag ttaattgtat tagcgtacag tgaagaagag gttcgagaac tcgttgaaac
     7081 acctgtcagg aaaaaggttg aaacaataaa tgagatagac gacagccaat acaatagcat
     7141 ctatattgaa acaagtcatg gtggatataa gaaattagca actttcctta gaagttctaa
     7201 agttgggata atttctaaaa aagacataag tgctgttacc gatagttttt gtactttatc
     7261 tgttcctaaa gcttatttcc aatacgatag taaaagattt gaacgcttag agaaattctg
     7321 tagaccgtat actgtattga aaactaaaaa atttaataaa actaaaagag tttctataca
     7381 gttaccgact ggtcaaatct cattaggtga aaaagactat ctgataaaag taggaaaaga
     7441 gttaattata aaagtaccgg aacacttatt taaagaaaat tttgagatac gtgaaacctt
     7501 ggtatggaaa gaagaaaaat agtttggaag acatagaatg gtataagggg aaattgaaat
     7561 ggcagtaaat aaaatattgc aggtactaat catagacgaa tttggcggag gagaatggtt
     7621 tgatgtgcag tccatagact ttgaagaaga agtcgtagtt atagaaaata tttacggcga
     7681 agtatattca tattcgcttg aaggtgagaa tattgaggat ttacggttga ttggtgagta
     7741 gaaaaaaaca gaaatatcaa aatggagata aaaattaata gcatgtaaag atagtcatgc
     7801 ttgggttaaa aatgttaaaa ttcaaaaaaa tgagtcagaa gggataaaag tatgaaaaga
     7861 gaggaataca aacaaagatt aaacgaactg cttgaagagg acgaaacatt aactcatggt
     7921 tcgcctgatg aaattctata catgattgat aatatggtga tctttggtgg atatgaactt
     7981 ggtaataggt cagttgatca taatatttta gaatttgatg atgtatcatg ggaagaaatt
     8041 cttgattggg gaattttggc ggtacctgaa actaaaacct atattagtga cactatggtt
     8101 cctttttttg aggaattgga ttataaaaga cttcccaaaa atgaaaatca tatactggga
     8161 ggaaactaac aatgttaagt gacttattta ttttactgat ctatctaggt gtaagtgaaa
     8221 ttattgcgta ttttttgttt gtcattgcta attatggtat ttccagtgca atcgttcaaa
     8281 tgtttagatt accagtaagt gaactaaaaa aatgatgaga ttatcagcat atcttttaat
     8341 gtttttgaat gtggtcacgt taatctttgt agtaatcggt gtgactttcg atttaatgga
     8401 atagtgggaa atctataatg gaaaaaagag acacgaatga acatattttt tgaatcaata
     8461 ttgttgatgg tttttgcgat cagcgcttac cagaaatcta aaaaagagta atgattgttt
     8521 aatcatcact ctttttttgt agggaaattg aaatatgtgg agcttgtatt ggtttagtta
     8581 ggattgcttg gctataagga gctagttctt tatcaaaacc atcaaagtag gttttcagta
     8641 gcgatttaac tgatcgctca tcttgtttga tttggataat ttcagcatcc aaatcggttg
     8701 tgagatcagg agcttttgat gcgattacct gatttttttt atcttccaac tctcttaggc
     8761 tgtttaacaa gttgtaaaaa gaatcatttt gattgttcat tccaacgtga gttgcaattt
     8821 taacagaagt ttcgtccgta ttgtcatcaa aagaactcat tggaatatca agtagtaacg
     8881 aggatgtttc ttttactgac tttttcttta cagcattttt tgtttttcca tcttttttgc
     8941 tctttactcg agatgttttt ggagctagtt taggtaaatc acttgtttta tcagtcattt
     9001 attagctttt cctttctatt aatttatgtt attatattat cataataaca taaaaaagta
     9061 cactttttgg gggcgtagtt attgaaaaaa tctaagagaa agattgccac attgcttatg
     9121 ttaatggtgc aatttacagg gattgcaatg agtgtaatca caataaatcc gcaagtgata
     9181 catgctgatg ggatagctac ttatgatgca caatttaact gtactactga aggtagtggg
     9241 tcaggaacag atagcgatgg aaacgatact ggaagtggcg ctgtaacagg tgatgtagcc
     9301 aaaaatattg aagatattta caaatacatg aatggagaat atggcttatc aggtgctagt
     9361 attgcaggta ttttaggtaa ctggaaacaa gaatcaggta ttgatcctcg agcagtagaa
     9421 gggtattacg gagatggaca gagagaagtt gacttcatca aacaatttac tgatggtctg
     9481 atgggtattg gctatggtca atggacagct gggcgacata cagcattagt tgattggtct
     9541 aagaaaaata atggcggttc ttggtggact acagaagccc aattggactt tatgttcaaa
     9601 ggtgatgggg gattcgtagg aatattaaaa gattatgctt taaatgcttc tgatgatgtc
     9661 gtagaaaatg cagtaacttt tcataaaaac tgggaaattt cagctgattc cgaatcagct
     9721 gtaatagctg tacgtggtga aaatgcaaaa gaaatctgga agtacatgaa aagcaagggt
     9781 atggacggtg caaaagatga aagtaagatc aataaaatat ccggaggttc aagtaccgga
     9841 gatggatcga ataattcatc aagttcagca tcaggaacag tagaagatgc ctgtgcaact
     9901 acggatgacg ctaattctgg ttctggatca attgggaaag aaggaaaaat taacggtaaa
     9961 aaaggtcaag taatagcgaa tatgacttat gaagaagctt tgaaaaaata tgggaaagcg
    10021 atcacgctac caaaattcaa agaaccagat tggtcaactt caccttttaa cggaatatat
    10081 caaggtcagt gtacagagtt gacgtgggct tatatgagcc aattatacga tgggaatcaa
    10141 ccgactactg gaaatggtgg ttatgtatgg gaaagctaca aatcggcagg agcaaaaata
    10201 acgaacaaac caatcgtagg ttatgggttt agtgcttcgg atggatggtt atgggctgtt
    10261 ggagatgcag ggcatactgg tgtagttata gcggtgtttg aagatggatc atttctatgt
    10321 gctaacttta atgttccacc aacccaagct ccaacaaggg gagtttcggt aacgttgatt
    10381 gatggagtgg acggatcaga taatgtgcat ttctttagcg gtgtaggaaa agctaaattt
    10441 gaaatcgaaa aataaattgg aatgaaggac gatatagatg aatagtaaag tgaaagatac
    10501 aattatcatt tctttgcttt ctggtatgtt attagcactg cttgttggaa tcctattctt
    10561 caataagcag aatcaggaca tagaagctga aaaacacagt gtgcatattg aagataagct
    10621 gacattcttt aaagaggtta aaccaaatga ggtggatgga ttacggaaag atttagaaaa
    10681 tgatattcaa gaaatgttga atggatcagt tcaaaataag tttgcagatc aatcaggtga
    10741 agaatttttg agaaaactat ttattgtaac aggtaaacca ccaattactg ataagagtag
    10801 tgagaaagaa aaaatcgagt actataagcc ttttactttt aagctaacaa actttggaat
    10861 taagacatct agtagtgggt tgtatgaagc attgaccgat tttgaagttc aatacaatgg
    10921 aaaaaaagtg tatgatacca cattaaccat cacactaaat aatgataaga aagtgactgg
    10981 gggtgctttt tatgcaaaat catgaaatca ctactgctaa aaaggcgatc ggaatacttt
    11041 ccataattgt gatcctttgt tgtgtggtac tatttcaaaa gaaaaatgct tatgataata
    11101 atgtcaaaga attggaagac cttaaaagta aacaaatcga agtagtcaag aataaccaga
    11161 aagtgaaagc tatgcaggaa gctcaataca aagaattggg aaatgctgaa attaaggaac
    11221 aaagtaggaa attctttaat ttattttaca actggaattc atgggaaaaa tatacgaaga
    11281 atatgaagga aatacaaaaa gaatttcctc agatagtaaa gtctagtcct gttgatattt
    11341 ctggcacgat ggtaggcact ggtaatagtc caatatctag ctatactgct gattactata
    11401 caacaaatca aaaaggtaaa atggttgaat tgattacaca gacaagaaat actccaacaa
    11461 cagcaacaac aacaatttgg cgtggagtaa caagcatagg agaaaatcaa ttgttttatt
    11521 tggaggattt agatagctat gtaagagcag agtaaaataa aaaaagagct aacggtacca
    11581 acgttagctc tttgtgctga cgatgataac aaaacctacc gaaaaaggat tgtttcatca
    11641 caaacacaaa cagaaaagaa ttgataaaaa acccgaacct cagaattttt attaatcaat
    11701 tcatttagat tataataaat tgagctgaac attacaatac gtatatgttg taaaacgtaa
    11761 aaaaagtcaa aagtaaatac tttcttaaaa agtcaaataa aactattaaa gacaatatat
    11821 atggtttatt tgacataaga aaatggtcta gatgataatt gtaatataga attgtagact
    11881 taaataaagc ataaaatacc ttttatgata aggtatagct cttaatttta agcagtttta
    11941 gatataagat tgaaagtaaa gtgggggaga ataataatgg cagatcataa attagaaaag
    12001 ttattagaag aaacgtggaa tccaaaagaa tttagtgaat tttttatgga gaattttgaa
    12061 actgacttag cagtgattgt taaagatgct cttagagagc aagggtatcc tgaaactgca
    12121 aactatataa acattaattt tactttgtat actgaaaata aaggaacttg ggatttttgg
    12181 gctactttag caaataagga gttaagtgat aaatcagaca caggaattcg taactttttt
    12241 gaatcaaaca gagatgatta tatgtatgct aacaatcaaa ataaattgaa ctttagagta
    12301 gagtttgatg aaacacctga agaatttata gaaagacaac cgcctaaaga aaatgtagca
    12361 aaggtattgg aagatcgttg gaattcagat gaaatagttt caacaattag cgaactaggc
    12421 ggacaatatg aaccgctagt agaagcggtt agagaagagc taagactgaa taagtttcct
    12481 gatgttcaaa atattgatgt ttctcaaatt gaaataaacg tcaagatcac gaataaactt
    12541 gattatggta gttgggcaga tatagcatta gaaaaataca tctactcaac gttaaaagag
    12601 tttatcgaaa atagaatgga tatcatgtat ctacagcatc cacagtattt aaactttggt
    12661 gttgaaatag ctacacctct tgaagaatgg aaaatggagc aagggcttga ctagcctttg
    12721 ctccattttt ttattttaca aatgaataca gttcgtcgtt tttaccaaga atgattgttt
    12781 gcccaacttt gagattgtca ggtgatcctt cagcggtttg taaggagaac gttccattga
    12841 acttatcgga taccgttata ggtagcttta attctgatcc ttcctcatct ttgagaattg
    12901 ctgtttttcg ttcatcaaaa taaacagtct ttattaaata atttttcgta gtgatctcag
    12961 cagatttgtc tttttttgta gtagtaccag tttctttaga attagaggta ttttctacga
    13021 ctattccatc tttggcattt gagcttgtgt ttatttgatt gaatattttc agcgacaaga
    13081 taacgctaag aatagtcaat atagaaacaa gtgtaattaa tactgataat ttcctttttt
    13141 tgttcatttt ttcttaacat ccttttttgt tttgattagt tagatattcc ttatctaaca
    13201 agccttttta ggctattaaa tgaagataaa ccccaaaaaa atattgcaat tatgacagtg
    13261 caatggtact attattatac aataaatttt gcgaaagggg taggtctaaa tcgcaaacaa
    13321 aaagaaattg aacctagaca aatacaactt gtataccgca agggaagcaa gtttgatttt
    13381 gggtaaaggt agaaattatg ttacaaacat aatcaactat tatccaaatc gtttgcctaa
    13441 aggttcgttc aagaaagtgg gtgacaaaac ctatattata acggaagaag gaatagaagc
    13501 attgcgatca caaacagaaa ataaaaacaa aaagaaggaa tgaatatgaa gagaaattcg
    13561 cttggtatgg ttttattggc atcagctgta cttgcggggt tatcagtacc agtaacctca
    13621 catgctgatg aatggactgc aaacacaccg gatcaaatcc aaattacaac aggacaatca
    13681 agttatgcgt tcaaaaaagg agatactatt tggtcaactg tagagaagct taaaagctta
    13741 ggttacaaga ctactgttgc tgagtttgcc gaattgaaca atgttgattt ggcaaaagga
    13801 gaacaacgga ctatcccgat tggacgaact ttcaacatca gtaaagatac attaacagtt
    13861 aaggaagcag atggcgaagt agttagtcaa actgttcaaa aagataaacc tgatcctatt
    13921 gaaaatacgg agcaagagaa caaaaaatcc gagactacaa aagaatcgga accaacagca
    13981 aacacttctg ataagacaga aacaagtgct tcaagtgttg atggagcaag ttcatctaat
    14041 acaaataatt ctacaagtga gaaagaagca tcacagggaa caagtgaaag tagcactacg
    14101 gaagctgata ctggaacatc aacaaataac tcacaatcaa catcaaatga tgcaaatgaa
    14161 tcaaccagta catcttctag tgatcaatca caaacaggtt cgagtgatga aactgacagt
    14221 tctacttcac caagtaaacc cgaacaacca actaatgttg tgctaggcgt tccatttatt
    14281 agtcaaggta acacaatgct ttgcgaagga acatctttgc tagaagcgtt acattataaa
    14341 ggtgttgcaa caaatcaaga tttaatgact tttgttaaat caatgccact atctcctgac
    14401 aataatccaa ataatggttt ttctggtgaa tggagacaca atgtggatgg aacttatcaa
    14461 ggtatgaatc cggctcctgt agtttcatgg ggtgttgcta atggcggaaa tgttgtagat
    14521 atttctggtt catcggtaca aggattaaaa gatgagattg taaatggaaa tcctgtagta
    14581 gcgtgggtaa cttatgcctt tgaacctgct caacataaac caatgttttg gggcgatgct
    14641 atttggaatc gacatgtagt agccgttgat ggatttaaag acggttttta ccatgttgtc
    14701 gatcctgtgt ttggggcaag ttggattaat gcaggatcat ttgaaacagc atataacgta
    14761 tctcaattag cggtttcggt acgttaataa aagatatcgt ttatatacta agtagttata
    14821 tagtctttat ttgtccttgc ttaactactt agaaaacttg ttgctcatgg tgggtagaac
    14881 taaaaggggt gatgctatga atactagagc gttgaaacag cttgctttca acgaaaaaaa
    14941 tgattaatag gtgtggtgat tttaacttag gaatgggcgc gaaggtcatt accacaccga
    15001 catatcgtgg ctcattttct tcgcctaact ggtataaaga aggagtaacg agagatgaag
    15061 catgaaaaaa cgataatttt aaaatcaaaa ataatttatt gttttgcgac tttagtaatg
    15121 atgttatcta cgcttataat gcctttttca gcaataagag ctgaagcaga tgtcacaaaa
    15181 ccgacaagtg caaaatttat agaaactggc gtaaaaacag acggttatat tagggttaat
    15241 atgccgaatc atccaaatga atggatgatt tcaagtcaat ttaaagattc tcatggaaat
    15301 attggatatt gtatggattc agaattgcca tccccaacag gaagtggagc cggaagcttg
    15361 aagtacaagg gagctggcag tgatgagttc tatcgtatgt ttaaaggtgg ttttccatca
    15421 aaaacagcaa aggaactggg tgcaggaaat gatacagaag catggtatgc aacacaatta
    15481 gtttcatggg ttttagccgg aaactttaaa gtttcacaga ttgtatggtc acatccaaac
    15541 cataccgccg cagaaacagc tcgtgtaaaa aaagcatttg aaaaaattta tgactatgct
    15601 aagaatggaa aagatacgcc caacactgag ttttctatca cagcttctaa gacagctgac
    15661 gaaggaaaat atcacacttt cacatataaa acagcatcaa ataagactgg taatgcgaag
    15721 ctcactttta catcagcaaa accagctggt atgaagattt atgatgcaga tggtaaagaa
    15781 attactaaca acactgtgaa attaaattca agttttacta ttaaagtacc tgtgactaca
    15841 ccaagtggta ctttgtcatt taaaggtact gctaatgtta gcacaaccaa tccttttact
    15901 tttgatggac gaggtgtgta tcaagatgcc gtagttatga tcactacttc agaaacaaag
    15961 gactctaaat ctttgagtgc taaatggaca agagctgttg gtcaggtaac agttaagaaa
    16021 gttgatcaag atggaaatgc tttaaaagga gcggagttta ctctaactga caataacaat
    16081 gctaaatctg ttaagacaac cggagatgat gggttagcta cgtttgattt gcaacaaggc
    16141 ttaacataca gcttagaaga aacaaaagtt cctgaaggtt atcaaggttc atttaaaaag
    16201 gatggtatta ctgttaatac cgatggacaa gaatttagtt acactgctac gaacacaatt
    16261 aaaaaaggta aagtaaaagt taaaaaggtt gaccaagatg gaaaaccatt agctaatgct
    16321 gaatttacct tgacggatga ttcatcccaa acacaagcag aaaaaacaga tgaaaacgga
    16381 cttgcaagct tcgatattgt agcaaacaag acctacagcc ttgaagagac cggagtacca
    16441 actggttata caggcgtatt caagcaagaa ggaattaccg ttaaagatga tggacaaact
    16501 tttgaatata cagctacaaa cactttgaaa aaaggtttag taaaagttca taaagtcgat
    16561 caagatggaa aacctttggc aaatgcagag ttcactttaa ccgatgaaaa aggtgcaaca
    16621 caagtagaaa aaacagatga aaatggactt gcaagcttca atattgtagc aaacaagacg
    16681 tatagcctag aagagaccgg agtaccagta ggttacacag gtgcatttaa gcaagagggt
    16741 atcaccgtta aagacgatgg gcaaactttt gaatatacag ctacaaataa tttgaaaaaa
    16801 ggtgtagtaa aagttcataa agtcgatcaa gatggaaaac ctttagcaga tgcagaattc
    16861 accttaacgg atgaaaaagg tgtaaaagaa gttaaaaaga cggatgcaac tggtctggct
    16921 acgttcaaca ttattgcgaa caaaacttat aacttagaag aaacaaaagt accaacaggt
    16981 tacacaggcg cattcaaaca agaaggaatt accgtcaaag atgatggaca agtctttgaa
    17041 tatactgcta aaaacacttt gaaaaaaggt gtgatcaaag ttcataaagt ggatgatcaa
    17101 gggaaagcat tagcaaacgc agaattcact ttgacagacg acaaaggtgt aaaagttgta
    17161 caaaaaacgg acagcaatgg cttggcaaca tttaatattg ttgccaacaa aacgtacaaa
    17221 atcgaagaaa cacaaaaccc taatggttat acagggaaat ggtctaaaga aggcatcacc
    17281 gttgctgacg atggacaaac ctttgaatat acagtaatta atcaccttgt acctgaaaca
    17341 gtaagaacag gcggaatcgg tacatcagcc ttccttcttg tatttctaat tgcaggattg
    17401 agtatggtgc tactgtatta tcgagaacag cacaaagcaa aataaaaaag tgttaatgga
    17461 aaagaagtaa atatgaaaaa agaaggctga cgtatgaaat caaaaaaaat tttttcaaat
    17521 ttaatgctat ttggtacttt gctaggagtg ggattgggcg caagctcaat ctcttctagc
    17581 cttggactag gggggcaagt agcatatgca caacaaaact ataattatca aggaactgga
    17641 acgattcata tccataaagt aacaaatact aatggggatg ggaaccctgt ctcagatatt
    17701 gataacaaag ggaaagaaat agctttacca aatggtgtaa aacctcttga aggcgttgag
    17761 ttctcttatg tgaaacttac taaagatgaa gctaaatcct tgggatggac ggatagtgat
    17821 actacaaaag gtcaacatat tgatgatgct aaagcacgat tattactaaa caaaaaatac
    17881 agtgaagtaa gtagaatgaa gactgattca caagggaata tagacatacc tatacaagta
    17941 aatacagacg atttaacaga taacttgtat ttgattgttg aaacaggaac agtcaaaggt
    18001 gtagttgaag aagcgtatcc tatgattgtt aacgtacctg aactaaacgg atcagatgat
    18061 caatacttta attatgatgt acatgtctat cctaaaaact atttccaatt aaactcagca
    18121 gagcttgata aaaaagacga agatggaaag gctcttgccg gaactgtttt tgaattgcga
    18181 aaagatgatg gatctgatca ggttgttgga acttataaaa cagacagcac aggtaaatta
    18241 actgtagatg gtttggaatt aggtgactat tatttcctag aaaccgattt gggaacagat
    18301 gctaataaaa caacctattt actagatggt aaaaaattca aatttagcgt taaacgtggg
    18361 gagactgcta aagtagttct tgatgttacc aactacaaac gacctgtaat tactaaggta
    18421 attgataaag aaaccgttga tactggcgaa gatgttcaat atacattgaa accatcaatt
    18481 cctggtaata ttggtaatta cgaaaaatat gttgtcaaag atgttctagg tgcagagtta
    18541 acttatgatt catcagaagt gatcgaagga atgactttag atactgatta cagtatcgaa
    18601 cgtgacggac aaacgacaaa ctatgtcttt actgaatcag gtatggataa gcttcagaaa
    18661 caatttgatg caggggatcg cttctttaca attttgatga acgctcatac taatagtaaa
    18721 gctaaaatgg atacaccact taagaatgat gtttcaacga tctacaacaa tggatatgaa
    18781 cccgaagacg aaacaccagt tgatcacgct tatacaatca ttggtgatca ccaattcatt
    18841 aaaacagatg gagatacaag caaagcattg agtggagcaa aatttgtagt gaagaacaaa
    18901 attgatccga ataaagttac ttacctaaaa caagatccaa caacgaaatc tgttacttgg
    18961 attgcagata aatctaaagc tactgaattc gtttccggat cagatggtaa gttccaaatt
    19021 gaaggcttgc aatataccta tgatgtagat tttgataaac aaggtaatcc aacaatcaca
    19081 gataaaaaaa tcaatgatta tgctcttgaa gaagttgttg ctccttcagg atatacacca
    19141 actagaaatg atgttccatt tgtggtatcg aatgatgatg cttctagcga agtaacagac
    19201 atcaagaacc ttaaaacacc tgaaacagtt cgtacaggtg gaattggaac agtagtattt
    19261 ctagtggttg caggtgtggc aggaacaggg ctatatgttg tctacaaacg agaacaacac
    19321 aaagcttaat gaaataaaat tccctagagt gtttttggct ctagggtttc tttttaaaaa
    19381 gtgtgtgaaa agtaggtatg agcatgaaaa agaagaatat caagttgatc atcttaaaaa
    19441 ttgggatgtt gctatgttta ggagtaggtc tatatcctgt aattactcag gcttatacct
    19501 actaccaaac tcaacaaaca ctaaactcct ataaggaaaa atatgatgaa gcttctgaaa
    19561 aggagaatga agaagtcttc aagagatatt cagaagttaa taggaaacga aaagacacag
    19621 aacaaacctc agtcacgatt cctgaagata ataacaatat tccgtcaaat ggaaatcttc
    19681 agaaggataa attaggtgaa ttagtgggtg aaattcttat accaaagatt aatgtggatt
    19741 tgccaattta tgatggtgta aacgatttcc aactatcagc aggagcagga atattaaccg
    19801 gaactgatta tccaactggt gggattggaa accattctgt tatatcagct cattcaggca
    19861 ttcctaataa aaaatatttt acagatttag agttgctcaa acttggtgat aaatttttta
    19921 ttgatatttc agggaaaaaa cttgcttatc aagtagatcg gataaaaaca attctaccaa
    19981 ccgaatttga ggatttgctt cctgtggcag gaaaagatta tgtaacactt atgacatgta
    20041 cacctgttgg agataattct cataggttac tcgttagagg ggttagcata ccttaccaaa
    20101 aaagtattgt tgagaaagaa aagaagaaag aagatttaaa ggtaaaggcg cagatgaact
    20161 ggtatcctat cgtagttgta ggggttttat taataatagc tgtgatagtc attattaaaa
    20221 aaatagtagc aaagagacga ggaatgaaaa atgaagaata aaatactact ttttttgact
    20281 cttatttgca ctttggtttt aacaatttat cctgcaaatc agctctatca tcaattcaaa
    20341 acaaaagaga atgtggcgga aatccaagag aaaattgaga aaaaacaaac tccttcaaaa
    20401 gaggaagtgg caaaaaaagt cgaacaaata aaatcgaaca gcggtaacca aggattagac
    20461 aatactcaga aaacagataa aggagtagac aatactcagg aaacagacgg tggatcattt
    20521 gatattcgtg aagctgaagg aaaggtcaat ccatacacag gagatgtttt ttcaaaaaat
    20581 caaacgaaag attttgaacc atctacctat tcaggagcgg ttggttatgt ctatattcca
    20641 aaaataaatg tgctagagct tttgtttcta ggcgcttcac aggagcattt atatagaggt
    20701 gtggcacaaa tgagtgctac tagtctacct ttatcaggta aaggtcatca aacagtaata
    20761 gcaggtcatc gtggcggtta tacgtcacca caatttcttt atgttgatca attagtagcc
    20821 ggagaccaca tttatataga ttatcttggt gaaaaagcag tttatacggt tttccgaaca
    20881 gacgttatta gaaattacgg tcagaattca acactgactg atattcctga tttagatatg
    20941 ttaacacttt atacttgtca tccatatcct cagaactacg atcgtttatt agttagaagt
    21001 ctaagagatg aaaaagcaaa atacatcacg ccacaagtaa cagttggcaa taccgcatac
    21061 gctgatgcca ttgatagcga gtccgtttca gataagacag aaactgttac atcttctaat
    21121 gatttatttg aagatgttga tcgtgccaac aaggaattag atggaaataa tgatcttaat
    21181 attccggaac aaggtagcgt agagatgcaa gacagaacgt ttgctgaaaa ggcaagttca
    21241 atatttacag gaattaccaa taaacatcag aaaatgcttt tagtcatttg gactgtaata
    21301 ggagcattag caagtctaaa ggttcttatt cttttaatca aagagtttcg aaaagcataa
    21361 tgaagattat aaaaattgat agagtacagt aaaaagggat tgatactatt attgatctct
    21421 tttttatttt gaatagagct agattcactt ttaaataaaa tcaaaactga tcttaattct
    21481 aaagaaaaag tcaagaaaaa aatacaataa atttttttat cagaatattt atcagaatat
    21541 tattagaaaa gataaactca aaagcttaat ttaatagggt tattggttgt atttagaaat
    21601 aatgtatgga aaagctataa agcaatagat aagatgtaga aaatcttatt tgtgaatgaa
    21661 aaacaagtca tcgtaaaata ttaataggag gtgtagtaat ggtgggagta tcaaaaacag
    21721 ctttagactc attagctaaa ctatttagta gcaggtttga tggtgcagaa tttgttgaat
    21781 atctaagcca aaacgaaagt ggaaaaactc tgatcgaaac aatgaaaaaa gactatttga
    21841 atgaaaatga ctttccgaca tggacgaata ttgaagatgc tacatgggat gtagtatgta
    21901 cgatatattc tccttgggta acgattgctg attttgtttt agataaaatt ggaggggata
    21961 cataccctga tgccaaatct cttgctacaa gtgactttaa aacgttctat ggttatctaa
    22021 taggtctaat aggcaatgga atagatttga aagttacgcc aaaacaaaca cctgaagaaa
    22081 ttgaaaaaga aaaaggagag gttcaaatgg cttatgcaaa tgacagagaa aaagtaaaat
    22141 tattggaagg taaattatct gaagaagaac ttggaaaaat ggtgcaagaa atcaatgaag
    22201 acagcaacta ttttcctgaa ttgaaggtat atcaaaatga cgaagatttc tttaatgaaa
    22261 tgtttagtaa tagtccatgg gaagcagtaa gtaaaacaaa tttaggagaa tggaactttc
    22321 aagatgatct agttgcattt agcaatgatg ggtatttacg atcatattca gaaagtgagt
    22381 atagagctga attaaaaacg tatgaaacgg atattctgat gagctattta aatctattag
    22441 agagtggtca gggagagtta agagataaag ttgaagaact attaaaagaa gagctatagg
    22501 gattatttca acataaacac aagcagaaaa ctaccaaaca aaacgtacca tgcaaaaagt
    22561 cggttaaacc ggctttttgc tctattatga gaggagatca aaccatgagt gcaacaaccg
    22621 atatggcttt agaagcagta ataaaagata atacaggaaa tgtcgaaaat gtagaagact
    22681 ttagcgatat tgcaatcaat ctttcagcat ggatttcagc tgtaggagaa gaaaatatcg
    22741 aatttgagat caagaatatg gatgacttaa aagcaaaatt tccttttatt gcatcgaaac
    22801 taagtcaagt tgccgaggat aaatcgtgga atgaattagt tagtttttcc gaaaaaattt
    22861 atgatattac gcaagaagat gagtgtgttt catgggaaat gaataatcaa tacaagaaaa
    22921 ttgacgcttt cgtcaaatca cgtatagaag gaacagcttt tgagtttgaa ggtatagata
    22981 ccgcagataa ttggttaaat aaagaatcct ttattaaaag tatagcggat gatcttgaaa
    23041 aacaatggtc taaggaagac tatgcagagt acctaaaaac agaaagagat acagaagtga
    23101 aagaaaaact cactgaggta tgtttggatg aacatattga tcccaatatc gtaaaacaag
    23161 aagactatga agtggttatt gataaagttc ctactgattt tgaggaagtt gtaaagtata
    23221 tagttgaaga agcattatcg caatactctt cagatgagga agtggacgaa gaaactgttc
    23281 gtaccagagt ttggcaactg gtttttgatg acaaagatta taaatatttg agtgatcaaa
    23341 actcggttaa ttggagtgta cgattattaa ttgatcaaaa tgatattgaa taaaatagag
    23401 ctgaaaagct ctattcagat tgtagacaaa aaggagttgg taattaagcc actccttttt
    23461 gttagtcttt gcataatttt gaaataaaaa gccataggct tttttgtttt ccatagccat
    23521 gtggctagtt tttttaaatt catggcagca aacgtaagca tcgtgtgcgt ggcgactttt
    23581 tcaagacctc gatatttcgt ccaacgcata ccatgctttt cttttgcatc tgcaaatatt
    23641 cgttcaatgg tttgttttct ctttttgtaa atttctctat tcaacgccgt atggcgttgg
    23701 tgttcaagtt catccataag atcagcccaa atatgtcgag tcactatttt ttgatgtttt
    23761 gtactcaaag tacaattttc aagtaatgga caactcacac agaattttgg atttgattta
    23821 taggttcgat aaccatcacg agtgatagtt gaaaataaca acggttgatt attgggacat
    23881 aaatagcagt caaaataggc atcataaaca aagtcttttt tacgaaacat tcctttcact
    23941 ccatgaggtg cagtatatgg aagaatcggg tagagtttca agtgtgacag aaaatgaaca
    24001 aatttaggcg tcttatatcc tgcatcagca accacacgct ttatatttgg aaaagctctt
    24061 ttcgactgtt taaccagatc gattgcgact tggctgtcat gcacatttcc tggtgtgatt
    24121 aatgtagata agataaaacc gttatcttca caggaagtat gtgcagaata agcaaattgt
    24181 ttttctcgtt cacctttaac atagtaacca ctttctggat ctgaatgact tattttttga
    24241 agactcaact cactttctgt gtcccatgta aaaggacgtt ttcctacagc aatacgttct
    24301 ttatttattt catcttctaa ttcatcttga taagcttttg cttgaccgta ggtcatctct
    24361 ttcgtgaact tatgtttatt ggcatttgct ttgatatgag tagaatcaat ataaaggttg
    24421 tcttccgtaa cgaagccagc ttttacagct tgttctagaa tatgagagaa aatatcttca
    24481 aataaagttg tttctctaaa tcttcgaaca tagtttttac caaaagtaga gaagtgtggg
    24541 ataggatcct cgaatgaata acctaaaaac caacggtaag caacatttgt ttcaacgtct
    24601 ttaattgtct gtctcatcga acggatacca aataaatatt ggataaatac taatttaatt
    24661 aagaccactg gatcaatact gggacgacca taggttgaat acgtagattc tacaatggga
    24721 taaatgaaat caaactgaat cgcagcatca atttttcgaa ctaagtgatc tttaggtaca
    24781 agatcatcaa ttgttaacat agcaaattga tttctacctt caatcgattg ttttctcatc
    24841 attagaatca ctcccagaat agtttataca attagtataa aacaaaaaac tgcaaacaac
    24901 actccaaatt taggagtttg tctacagtct gaatagagct gaaaagctct attttttttg
    24961 ataaaaaaac tagctatttt attaaaaata atttggttca aaatttattt ttttaacttg
    25021 ataatttttt gccatccttt tttaaattgg ctatatatca aggtttatag tgggaataaa
    25081 taaaaataca tttgacaaaa tttacaatat agtttattat atatttatct tattatgata
    25141 acacaataaa agtaatggtg gtatgaaaaa gaagaagttt agccgatcgt tgttaggaat
    25201 tttggtttta gttgcggttg gtcttagtgg caagggttac tttcagaata acagtactca
    25261 ggaacatgat tttcagatca aaagtagtgt tcaggtaggt aataaagcag acagtgaatc
    25321 aaaagaattt ttagggatac gttatgatcc aaacaaatat ccaaataata tcgtaaacat
    25381 taatgatggg aaatatacac tgacaaaaga agaccatgaa gcattatcag aaaaaggcag
    25441 tcaaaccttt tgggtaaatt atgcagattt agatcaactt ggaagagcag gagatgttga
    25501 agcgttgatc acctatgatg ctgtaaattc tcactcatct aaaaatgtgg aacgtccatc
    25561 tttcaaaagt tctgtccata ttgcaggaga atacaaggat ggagtatttg atcaagagag
    25621 agatacttgg gtcggcaaag attcaaacaa tgaaatcact cagctagaag gctataaagg
    25681 ttatctctat aacaaaagtc attcattagc atggtcgcta ggaggagata tggaggtaca
    25741 caatttaaca cttggaacga gagcgcaaaa tgttgggaaa aatgatcaga atggagggat
    25801 ggcttactca gaaacgattg tacgtgatgc aatctataaa aataagaatg aaaaagtatt
    25861 gtataaagtt gatccaattt ataaagggaa tgaattagtt cctcgtggtt ctcatgtcga
    25921 agctttctca gttaacgata atggaaaaac ggtaaacctt aatgtttatg tatttaacaa
    25981 tcaaaaagga atcaacatca actatcaaaa tggtacttgg gtaagagatt agaaattgag
    26041 aaggtgaaga attggaaaca ataagcatca atggattaga taaatcagtt gcaaatcgaa
    26101 taaaaaaagg aaatggctac tatcttctga agaagggtaa atattcagtt gctagagagt
    26161 tagaaacaat gagtatcgtg tatgtagcta agaatcattt tgacgtagaa caggctattt
    26221 ttgatccaga ttctgaaata gggaaatgtg ttgaattatc agagaaaact gttagtacaa
    26281 aaggggcgat gatcattgag agagaaatgt tagacgaaat cagagtaatc attcctaaag
    26341 atacgcagat gtgtgaggac agcataagtg acgatgaacc aaaatcagaa agaaaataag
    26401 gagatattca gatgagcgaa aaatccattg aattaaagga atataagcga agcgatggag
    26461 aaaaatatta tcctgtagcc agtttttcta aatcagtata tctggttgga cgtgcattgg
    26521 tagttggtgc aattggcata tatattttgc attgggtaac aaaggttgta caactgcttg
    26581 tagatacctt agtaagtatt cttgtctcgt taaatgatgt taacggaaca gatgatttag
    26641 gattaaaaga gatgtttaaa gagttactca atgcgttttt tcccaattat ttaagttatt
    26701 catataatgt acaaagcttt tttgcaaagc tacacttggt agacatctct attttagtat
    26761 tagaagcagg aacggtattc tttgttggat atttggttac tttgatgttc aggcatcact
    26821 atggagaaca aagaccattt tttgatgata tggaaagtag aaggttgaaa agagaagctc
    26881 tagaagctat ggatgtgaag tatgatccaa acaagattat aggagatgaa acgaacaaca
    26941 aaaaaagaaa acgaaacaaa acgcaattag aatccgatgt gctatcaacg cttaaaaata
    27001 tcaaaatttc tgttcataca agacgtgatc taagtgtcaa tgaagatatt cgagaatata
    27061 tcgtaaaaat cccgcaacca aaagggaaga aagttagaga aacagtttta cgacaaattc
    27121 aagatttagg gtcgatcctg acaagagcaa caagcggacg agccaaattt ggtgaaaaag
    27181 aagatgaagt ttcaggcgat tatttcattt tccgagcatc agtaaatatt gaaagagaac
    27241 gtaaggaaag agtaagtacc aaaaacaaag aaacggaaag agcaacatct tcaatcgcag
    27301 tttcaaattc acaattttct tttccaatca atctctttgc cgataattcc aaaaatatcc
    27361 aaaaccaaac cgaaaaagct agaaaatatg ccgaaactca aaagcaatct ttagaacttt
    27421 atctatcttc tgctgacgta cctgcaagtc taacagatat gcacgttggg aatacatcta
    27481 ttgaatattt gtttacttta tctcgtacca gaaaaattca actaagctca atgaaagaag
    27541 aaattgaaac ttacttgcat atatcaggtg taatcgtgcg gatcaacgca gggcgtattc
    27601 aaattacttt accattagga gaaaatgatc gtattccaat tgatgtacca tcaatgatca
    27661 atagcgtgtt ttagaagaca tcacaaatag aaacagaaaa tacaaactac cagttcacaa
    27721 acagaaaagt accgagaaaa gtgaggaata gaaatggcta tgaaattatt agagaaagtc
    27781 aaaaagagag ccgaaaaagc tgaatctatt ttgctagata aagaacctaa tataaagctt
    27841 aatggaaaat tcagtgtcaa tatgttgaac acaaaggcta aagacccaac agatgctatt
    27901 tttggaatta agcctgatgg cacaattgct catttttgca tcagcgaaat gccacacatg
    27961 ttggttgcag gaacgacagg atcagggaaa tcagtattac tgaatgaaat tcttgttaca
    28021 gtaatgtgtc actcaacgcc tgatgaatta aaaataggaa ttgtagaccc aaaaagggtt
    28081 gaatttggaa gatacaaaaa attgccgttc atgttggctg atccaattac agatatggat
    28141 gaagcttatg atttttttga atatcttgtg atcttgatgc atgaacgtta caaactgatg
    28201 gaaaaagcag ggttacaaaa catcacgcta tataacgaat atgccgaaaa aaatggatta
    28261 gatagatttc cctatgtgat cttattagtt gatgaatatt cacagttggt tggaacacat
    28321 aaagaagtag aaggtttgat tgttcaatta ggacaaatgg caagggcagc aggtattcat
    28381 gttatcttag caacacaatc tcctcgatca acagttgtca ctggtattat caaagccaat
    28441 tttccgtcac gtgtttgcct tatggtagca agtgatttag aaagtaggat tgtattagat
    28501 gaaggcggtg gagaaagtct aagtccaaag ggagatatgc ttattaagct tgttgatgga
    28561 agtatggtta gagcgcaggg agcttacatc agtaacaaag aaattgaaac aatcttcaat
    28621 caccttagaa atactatgcc agaaccagaa tatgttgatt accgagcaat cgtagctgaa
    28681 actcgtggtg agggcggtga agacgaggat tttggtggaa ttggtaaagc tcctgtaaga
    28741 aaatctgcta aaagaaattt gaacacacct ttagcaaatc catcatctaa agagccatca
    28801 attgaaaaga agagtgcgtt tgctattaag cttgaaaaga acaaagaaga aaaagtcgag
    28861 cagggagcta aaacactaga aaaactgcaa gcagaaaaac tagaaaaagt tttaagggca
    28921 ttaggaaaag ctgatcctga accgcaaacc tcaaacaaca cgaataataa acagccgatt
    28981 gcatctaaaa ccaaacctaa agctaatgat ccaaaaggct cagaaaacgt agtgatcaca
    29041 actgataaat ctgaaaagaa acaaataaat ggaagtactt cacctatcat gcaaaaaatg
    29101 ttaaaagcaa aaaaacaggt gcataaatga tcatcgtcag ttctttttta agatggctta
    29161 ctccatataa atatacagat gaagtagttg tgaaacgagc gaagaaaaga gataaaaaag
    29221 cggtactaac tcctaaacag atgcttgatc atggcatttt aggggtgaag gttttagaag
    29281 tgaataaacc gtttgaatca aatagtgcaa gagaaaagtt gcttggagct gaagccgatg
    29341 tcgcatattt ttaccatgac aaaagcaagc atcaaatagc tattaaattc aatccaaaat
    29401 cggcatggcg gtatcaagca agagatgact tgaaacgtag gaaacttaga ccaatgctct
    29461 atccagaagg aaaagaagca tttgatagaa cccacatcgt tcccattggt tttcatggta
    29521 cagaatcaga taaccgccta ttagtaggtt ggaacagcaa tcaaaataga gttcagatca
    29581 ataaatttga gaagaaagta aggaaattca acgatcgaaa aacgatccta tggtttgcag
    29641 atatttgtaa acagccggat ggaacagcaa agtgggaagc ggtgatattt gacgaaaatg
    29701 gtaaaaaagt aatgtctgaa acatggcatg ataaagctaa atttgtttgg cagtagcagt
    29761 agaggtgaaa aagattgcta gaactttttg aagcaaatag aacaggcttt aatgggattg
    29821 gcttagatcc aatcatcaag aaccgtagga actacgggga tagcttggta aataagagac
    29881 accgctgtta gtaaagaaat acgctatcaa gtatgctcta ttcccaagaa gctcccactt
    29941 caagcgttaa gaaccgtcag gtttagctaa gtggtgagta gttcactaga accttggtca
    30001 caggcagaaa ttgataaagc agtaaaattg aaccgattat caaaatttaa cgtattagga
    30061 aatgcctttt aaaaatcgca atacacaaac agaaaaatac caaaaaatta gcaatgaacg
    30121 taggtaagaa catgacaata aataataata accctaaaaa atataatgct cttgtccaat
    30181 gctatatcga aaacaaaatt acaatgcaag aagtcgctga tgaattaggc aaatcaaaga
    30241 gcctagtatc acacgatttt aagcacaatg tttttccgaa cccgattgag ctgaggaaaa
    30301 aatttcaaaa gagagtttat caagaaatag gtaagtggat tgatgaaggg aatgcaattg
    30361 accctttaat tgaaaaatat ccttttttaa aaagcaaaaa taaaattcaa aggttaattc
    30421 gtcaaggcga tattcaagct aaagaacctg ttttgacaac caatgatttg gctaaaataa
    30481 atcttcataa acaaattatt gaagactttg aaaaaaatct ttcaacaact gaaattttaa
    30541 aaaaatacaa tattccgaga ggaaagctaa catcgactct ttttagagaa tatggagaat
    30601 cggcagttac taaagcaaag gaagtagcga atgagagaaa aaaacaaaaa tagtagtcga
    30661 tacctagtag cgactgtagt ttttgtttgt gtattgattt tcctgtttga ttttttgcag
    30721 attggaaaaa gcataattgg aaatacattg agtgcaaagg aaatggttgc tttaggtggt
    30781 ttgtatgaag gacacttgtt cccgaatata ttcatttcta tcgtttctca tattaacttg
    30841 taccatttaa tttctaattt attgttctta gtagttcttg aagaagcact gaatgatcag
    30901 tatgagtggt taacattatt gatcgtttta atcatatcag gatttctagg aagcttagtt
    30961 ccttttctgc tacacagcaa catcgtaagt atggggctta gtggaggtat ttttggatgg
    31021 attgctttat ttgcgattgc atacatccgt tttgatagca agaaacattt attactaagt
    31081 gtttttgtat tattgataat cggcatttta ttggtatcta cttttgaatc aggcgaggca
    31141 aatgtcttgt cacatgtaat cggatttgtc tgtggaatga tcttgggatt tattctaaag
    31201 aacgtttcat tgatcaaaca aaagaattaa aagggagtgc taaaaatgcc aaaaagaaaa
    31261 gaaaagaaaa ttccagtgtt tttgtctgtc tcagaaatgt tatcacttca aatactgtta
    31321 agaaaaaaga ttgaaacaat agacgaagaa gttagaaata aaagggctat gtacgaacaa
    31381 cgtgaagcgc taagagaacg aatccactat tttgatgata tacacactaa aatctatgaa
    31441 gcacgtacaa atcacgaaga cgaaagtaaa ttaggattag ctcaacaacg gatacttgaa
    31501 tggcttaata cgaatactga tcaagaaccg ttaagagtga ttagtaatct ttttgaattt
    31561 ggaacgaagc attggtttaa tgctgtagag aaggatgctg acttacaaga agcgtttgat
    31621 acgctaagta aggatagcaa atccatgcga gatttattga ttacttatct aaatgagcat
    31681 gagtttgaac aaacgaaaac caaaaagaaa aataaagaat agaaagttgt gatgaatcat
    31741 gctattaacc ctgttaggtt atttatgttt aattcttgct ttttttgggg caatcgctat
    31801 aattattggt caatttcaaa caaataatcc gattatgcgt aacataggca ttggaattgt
    31861 gattgtaacc tttattgtgt ttaatgccac taagattttt ggaagtaacg ataaacaatt
    31921 tggtatatcg ggagttaata atagttatgc tccagattct ttgggaaggg tagtaataga
    31981 tggaacattt gaaaatgtaa cgagttataa aaatgtttcg ctgacagtga aggaagatgg
    32041 caagaaagca tcagacgacg ttctcgctac tattaagccg tctcaatcac aatttgaagt
    32101 gttttataca gttaaagaga atagccctgt acacaagctg acattggttt ttaacgttga
    32161 tggagaagaa tactataaag atgtaaaaat aaataaaaca tcaacgaaaa agattatcga
    32221 atcagcgaaa aaagaagtag ctacagaaga aagtacaact gaatcaagtt ctactgtcga
    32281 agcggaacta ccagataaaa ttgattttga tcctgaaaaa gtcaaatcgt ttgaatctgt
    32341 ttctggttct atggctgatc ggtttaacac tgaagtcttc aaagctatca aaactgttaa
    32401 attggatact gaaaagcaaa tgattaatgt taccttattc aattcagttg aatctatgga
    32461 tcaggataaa aaagttacgg ttgcaagaga actaggaaaa agagtaattg gtttgtatcg
    32521 tggtactgct aaaccagatt cagacggtgc agatttgcca gttcatgtag tttatgaaga
    32581 cggtaaagtt tttggagatt ctacagcaaa cgaagcatta acgattaaat tggctaaata
    32641 gccagaaagt tataataaac aaatggtgtc acaccaataa tatataggtg tgacaccttg
    32701 gagatgatag ctgatggtag taaataaaga aaaagttaga aagtatgtga ctttaaaaat
    32761 agcttctgtt aaatatttag aaaagcgagc taaagaagaa gaaagagcaa gaggtaagaa
    32821 agtaacagtt tcagagttgt tagaagaatt gatcgagaat gatcaaacaa taagacgtgc
    32881 atttactaat aggtctatca gataacaata taattaataa aaaagaaaga gtgatttggt
    32941 cttagactaa atcactcttt ttatataaag tttctttaca ctcattccga ataatgttaa
    33001 aattctttat atgatatata gttccttgtc agaggaagag ataaccagca atattggagg
    33061 gataataagt ggaaaacaat aatgatatta gacattttag cattggggaa agagttacaa
    33121 cagtaagcga attatcaaag gattatagag ataaagaggg aacgattatt tctcttaata
    33181 agaatcgaag aggcgtttgg caatatgttg ttgatttagg tgatggcgtt acagttgagt
    33241 gtaaagatag tcaactgatc agaaataatg gcagtcattc cattgaaaaa gcaattcttg
    33301 tatacccaaa agtatcaagt aagatcatga atgactggtt gcttggaaaa tttggtgagt
    33361 acaataaaaa agaaatcgca gaagtgatcg gcaaatctta tccaacagta ttagggtatc
    33421 taaaaaagat aacgagtaaa gaagcaaaaa ttgaggatgt tttaacagta agagagcttc
    33481 aatcattaat cgagtatcgg aataaacaaa taacaaatcc tgagtacctt agaaaagaag
    33541 atggtctatt gaaacaattg gaacaagtaa ataaaatccg gaagatgcaa aaggtagctc
    33601 ctttaagcct tgataccttt aaaaaaatct atttgaaaga gatcaaaaaa gactaatgtg
    33661 gactgtaact ttaccgttcc aacctgtatc tgcaccaaga ccaagtgcaa gaaggaatgc
    33721 tgctgatgaa actaatgacg tagcagagaa aaggatttca acctactatg ataaaaagta
    33781 tcttgattat ttgaaaaaaa taaagagctt tatcgaagat caagatttat tagatgatga
    33841 attttactcg attgtaaatg atcctatggg cgcaattatg gaagtggact tttactacca
    33901 gataccaaaa agctataaaa aagtctataa cattatgaag acaacagcgc cagatttaga
    33961 taatttagta aaaagtgcta tggatggtat ttttaacatc tcagctatac gagatagcca
    34021 tatcacaggc ttaattgctt ttaaatataa tgtggcgaac gaaggaaaaa cagttgtgag
    34081 gatcaaacga tattcagaat ttgagtggga tacttctgaa ggattgaacg gagatatatg
    34141 ggaagctaca tttgatttta aaccagtagc aacgccaaga ccaaagtcta aatttagagg
    34201 aaatgggatt atcacttact atccaaaaga gtacaacgac tatttggaaa atatcaagaa
    34261 tacgcttaaa gagaaagatt tggtaaatga tcaactgaaa aaggtaatga atgctcctat
    34321 gggggttata gctcagatag attattttta ccaagtgaga aaagataaga aaaagcttga
    34381 ttcactgatg cgtacttcac agccggatat tgataattta gtcaaaggaa cattggacgg
    34441 aatttttaac aaggaaatag gcatcaaaga tagcagggtt gtagggttaa ttgcttttaa
    34501 atacaatact tttgaaaagt cgaaaacaaa cgtgagacta caggagatgg gctgatgaat
    34561 aataatgaaa aaattcttca tgtacttgat agctttgaaa taattcaaga agaactaaaa
    34621 aaatatcggg atgttttgga acaaagatat gattttgtca atcagcaaaa gagtaatcac
    34681 atggatttca ttctcaatat gaatgacttg aagaagaaat tagttgaacg aaaagaacaa
    34741 gaaaagttga tcaaagctta ctttgaactc ggtgaaaaag aagttaaaaa tgcaatggaa
    34801 ctgaatgaag agcgcagggt tttagatcag ctgttagaac aattattggt gatgtttcaa
    34861 aaaggacgaa tagatgagga tttaattgaa gaaggattga gaaaatatcc ttcaaactca
    34921 ggtatcggaa tagtgctgaa agcgattgat gaagaagaaa ttgaagcttt cataccgcca
    34981 gaggattttg aatcggcaat ggaatatata aaatattata gccaagggat cactgctttt
    35041 agagagtttg atcctgaaga tgttattcat gacttgaaca acctaaaaga atggtgtgaa
    35101 agttatggag tcgatgattc cggattggac tacttgatca gtattatgga aatcgaagaa
    35161 gagatgccgg ataagcctga tccaacagat attttagaat tgatccatga agctagaaat
    35221 ccaattgcct atatttctag aggatatact gtattggaat actataagcc ttatatatca
    35281 gcgatgaacc atttaagacg tgtattaagg gaaaaacgtg aataccgctc agtgttgaat
    35341 gcaacgaata gattagaaaa agctgtttca gagctagatg cttattatcg tgaacattac
    35401 ctgcaagcag gtggtatgcc aagaaatacg aaagcaaata tcagtaggta tataaaaaaa
    35461 gctgagtagg cggagttagc ccataaaatg gcaaaaggta acctgaaagg ggcattttga
    35521 atgaaaaggg atgtttttga attgtacgta ttagaacgtc tagaagacgg catgatggca
    35581 caagagataa ttgatgaaat tgatgatatg atcagcagat ttggtcaatt ggagttgaat
    35641 gatggaaaca aaaattcggt agtgctaaaa caattttggt tgaacaattt ttcgtataag
    35701 gattttatca aagaaaaggc aaatacattg tactgactaa attcacgagg agcagtgatg
    35761 agagcttatt gctaagagca gttaggctct tttttgtttg tgtaaaatta tttgttttat
    35821 aatacacatt tatacacaaa aaatattgac agatagtaca caaatgtggt aggatatatt
    35881 tgtaagcaag ttagattaac aagttaaagg aagagcagtt atgaaaaaag aagaactaat
    35941 agatatattt caaattgttg aacgagcaaa taatatggga attatgtttt ttgatcgtat
    36001 ttctttaaa
//
LOCUS       DACGLE010000020        35882 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0534
            SAMEA3304449-rid9560453.denovo.021, whole genome shotgun sequence.
ACCESSION   DACGLE010000020 DACGLE010000000
VERSION     DACGLE010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304449
            Sequence Read Archive: ERR985064
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 35882)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35882)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702225.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702225.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 20:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,045
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 27 of 67
            Pseudo Genes (incomplete)         :: 25 of 67
            Pseudo Genes (internal stop)      :: 19 of 67
            Pseudo Genes (multiple problems)  :: 4 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35882
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304449-rid9560453.denovo.021"
                     /strain="VREN0534"
                     /db_xref="taxon:1352"
     gene            <1..1152
                     /locus_tag="IXT20_001135"
     CDS             <1..1152
                     /locus_tag="IXT20_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005236379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4859593.1"
                     /translation="PSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHNSAPSTSAPSA
                     EAPKEKVETSGSASVVEAADPNKRVLAMPSVRQFAREKDVDISQVTATGKGGRVTKED
                     IENFLAGGPSSAPAKSEAPEAAAPKEAAPAAESKPAAPAKPFKSNLGDLEERVAMTPT
                     RKAIAKAMVNSKHTAPHVTLHDEVEVSKLWDNRKRFKEVAAANGTKLTFLPYVVKALT
                     ATVKKYPVLNASIDDANQEIVYKHYYNIGIATDTDHGLYVPNVKDADRKGMFAIADEI
                     NEKAKLAHDGKLSAEDMRNGTITISNIGSVGGGWFTPVINYPEVAILGVGTIAQQPIV
                     NAEGEIVVGRVMKLSLSFDHRIVDGATAQQAMNNIKRLLADPELLMMEG"
     gene            1160..2566
                     /gene="lpdA"
                     /locus_tag="IXT20_001136"
     CDS             1160..2566
                     /gene="lpdA"
                     /locus_tag="IXT20_001136"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HAQ4859594.1"
                     /translation="MVVGDFAVELDTVVIGAGPGGYVAAIRAAEMGQKVAIIEREYIG
                     GVCLNVGCIPSKALIAAGHHYQESLDSEMFGVTSENVKLDFAKTQEWKENKVVHTLTS
                     GVGFLLKKHKVETIEGEAFFVDDHTLRVIHPDSAQTYSFNNAIIATGSRPIEIPGFKF
                     GGRVLDSTGGLALKEVPKKFVIIGGGVIGAELGGAYANLGAEVTILEGSPQILPTYEK
                     DLVKLVEDDFKKKGVTVVTNAMAKEAVDNGDSVTVKYAVDGKEESVTADYVMVTVGRR
                     PNTDDMGLEQAGVEVGERGLITVDKQGRTNVPNIYAIGDIVPGAALAHKASYEAKIAA
                     EAISGKKVAVDYKAMPAVAFTDPELASVGMTIKEAKDAGIEAKAYKFPFSGNGRALSL
                     GKTEGFIRLVTTIEDNVLIGAQIGGVGASDMVSELALAIESGMNAEDIALTIHPHPSL
                     GEIVMDASELALGLPIHI"
     gene            2728..3003
                     /locus_tag="IXT20_001137"
     CDS             2728..3003
                     /locus_tag="IXT20_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0223 family protein"
                     /protein_id="HAQ4859595.1"
                     /translation="MQEYQYPLDLDWTTDEMVIVMKMWEDLERANEKGINNEAFLKTY
                     QQFKTVVKSIGEEKRLGREFEKASGYSLYRTVQQAKKNPNKTLRMNG"
     gene            3009..3776
                     /locus_tag="IXT20_001138"
     CDS             3009..3776
                     /locus_tag="IXT20_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HAQ4859596.1"
                     /translation="MEQMIDHIKSWLEEAGEKIKSSQQTLTISQKSNRTDLVTNVDRN
                     IQNFLIEKIHSFDPEAKILAEEEGCNQLSDLDGRVFIIDPIDGTLNFVIEGENFCIML
                     AVYESGVGKLGFIYDVMRNELFWGGKGIGVYKNDEPLPRPANKSLSEGLLGVNSYLFG
                     HNKYGIREIAESSMGTRMYGCAGLELVAILKGNHIAYISNLSPWDYAAGNVLLEEFGM
                     VTSGLFGTPLTFEKREYYLAGTSCAYAEIADLLKKEG"
     gene            3944..5773
                     /gene="typA"
                     /locus_tag="IXT20_001139"
     CDS             3944..5773
                     /gene="typA"
                     /locus_tag="IXT20_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HAQ4859597.1"
                     /translation="MNYRNDIRNVAIIAHVDHGKTTLVDELLKQSDTLDAHTQLQERA
                     MDSNALEKERGITILAKNTAVDYKGIRVNIMDTPGHADFGGEVERIMKMVDGVVLVVD
                     AYEGTMPQTRFVLKKALEQHITPIVVVNKIDKPSARPEHVVDEVLELFIELGADDDQL
                     DFPVIYASALNGTSSLSDDPADQEPTMAPIFDTIIEKIPAPVDNSDEPLQFQVSLLDY
                     NDYVGRIGIGRVFRGTIKVGDQVALIKLDGTVKKFRVTKLFGFFGLKRLEIQEAKAGD
                     LIAVSGMEDIFVGETVTPVDHQDALPILHIDEPTLQMTFLVNNSPFAGREGKFVTARK
                     IEERLMAELQTDVSLRVEPTNSPDAWTVSGRGELHLSILIENMRREGYELQVSRPEVI
                     EKEIDGVKCEPFERVQIDTPEEYMGSVIESLSLRKGEMQDMVHTGNGQIRLTFLTPAR
                     GLIGYSTEFLSMTRGYGIMNHTFDQYLPMLPGQIGGRHQGALVSIDTGKATTYSIMSI
                     EERGTVFVEPGTEVYEGMIIGENSRDNDLTVNITKAKQMTNVRSATKDQTSVIKKPKQ
                     LTLEESLEFLNDDEYCEVTPESIRLRKQILEKNAREKASKKKK"
     gene            6034..7806
                     /gene="recQ"
                     /locus_tag="IXT20_001140"
     CDS             6034..7806
                     /gene="recQ"
                     /locus_tag="IXT20_001140"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HAQ4859598.1"
                     /translation="MNDLLKQYFGYDEFRPGQKEIIQKVIDQENVLGIMPTGSGKSIC
                     YQLPALLLDGLTVVVSPLISLMKDQVDAANQLGIPATFINSSLDGYETARRFQEIDRQ
                     QYRLLYIAPERFIMPDFIQAMKRWNVRMIAIDEAHCISQWGHDFRPSYLQMANQLDQL
                     PNRPVIVALTATATVQVAADIKRLLKIPENNHIQTGFERENLRFQVIKDQKKEQYLIE
                     YLKINKNQSGIIYAATRKEVDRLYHLLKKFDFSVGRYHGGLNENERTEMQEAFLYDRL
                     QLIVATNAFGMGINKSNVRFVIHYQIPGSLEAYYQEAGRAGRDGLSSEAILLFSPQDI
                     QVQKFFIQQSQREEGQKQKEYEKLKAMTEYVYIESCLQQYILNYFGETSSPCNRCGNC
                     LDDREIVEVTTEAQMVLSCLKRMGENYGKQMLMKVLAGSKEQKLQALGFGHLSTYGLM
                     KNQSQKETMQLIEYLISNGYLLTINGEYPVLKVTERGIQVLKGQESVYRKEPKKVQQL
                     SDEETDTLFEVLRELRTDLASEAGVPPYVVFSDSTLKEMSRIRPSSRLEMLQIKGVGQ
                     SKLDKYGEAFLSRIKNADSNVLDK"
     gene            7920..8222
                     /locus_tag="IXT20_001141"
     CDS             7920..8222
                     /locus_tag="IXT20_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaN family protein"
                     /protein_id="HAQ4859599.1"
                     /translation="MDSLSKKLAIQVLNDEAERIKRLIRNQNNSLCISQCKAFEEVVD
                     TQMYGFSRQVGFAVRIGVIGKNEGQLMLSELERELNQLYSEVYQEIYDKKEVGKEE"
     gene            8225..9388
                     /locus_tag="IXT20_001142"
     CDS             8225..9388
                     /locus_tag="IXT20_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HAQ4859600.1"
                     /translation="MPKKVKKRHLLDYSILIPYLILCVLGLIMVYSSTSYLLLENGQN
                     PSASVINQSIFWVLSLIAIALLYKMKTDVLKNQRLIMAAIAVLTILLLIVVFFGKEIN
                     GAKGWLQIAGFSIQPAEYLKIISIWYLSLTLSKRQNSVQKDFLGTVKRPLAMVIGLTA
                     LVAILPDFGNAAVIFLIILVLLLASGVNYVYTLIVGVGGFCLSTFTIWLINITNGKIL
                     PGRLQYIYNRFAIYQNPFSDELNKGHQLVNGYYAMFNGGLFGRGLGNSIQKKGFLQEA
                     QTDFIYAIVVEELGVIMGILILALLFFMIVRIVLVGIRSKDPFNSLLCIGIGAMFLIQ
                     VFVNLGGITGVIPLTGITFPFLSQGGSSLLMLSICVGFVLNISADEKRKSLGL"
     gene            9414..12842
                     /locus_tag="IXT20_001143"
     CDS             9414..12842
                     /locus_tag="IXT20_001143"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase"
                     /protein_id="HAQ4859601.1"
                     /translation="MKKVLVANRGEIAVRVFRACTELGIKTVGIYAEEDEYSVHRFKA
                     DEAYLVGQGKKPIDAYLDIEGIISIAKECGADAIHPGYGLLSENLNFAQRCEEEGIIF
                     VGPKLHHLDIFGDKIKAKAAAIEAGIASIPGTDGPIASIDDALEFAKQYGYPIMIKAA
                     LGGGGRGMRVAHDEKSAREGYERAKSEAKAAFGSDEVYVEKYIANPKHIEVQILGDTH
                     GNVIHLFERDCSVQRRHQKVVEVAPCVSMNEQQRQKICQAAVQLMKYVGYVNAGTVEF
                     LVEGDDFYFIEVNPRVQVEHTITEMITDIDIVTTQLLIAQGLDLHKEIGLPQQEGIKL
                     NGSAIQCRITTEDPLNNFLPDTGEIDTYRSPGGFGVRLDVGNAYAGYVVTPYFDSLLV
                     KVCTHGATFETAIQKMERCLKEFRIRGVKTNIPFMLNVITHPEFQSGNAKTTFIDSTA
                     TLFEFPRLRDRGNKTMKYIGEITVNGFPGIESGEKPFYEEPRMPKDLITRTDYVTAKN
                     VLDAKGADALVEWIKGQENLLLTDTTFRDAHQSLLATRVRTKDFKQIARLTGEGLPEL
                     FSSEMWGGATFDVAYRFLNEDPWQRLRKIRSLMPNTLLQMLFRGSNAVGYSNYPDNVL
                     VEFVKEAAAQGIDVFRIFDSLNWTPQMEKSIQAVRDTGKIAEAAICYTGDINDPSRAK
                     YNVQYYKDMAKELEQLGAHIIAIKDMAGLLKPQAAYRLISELKETTDLPIHLHTHDTS
                     GNGIITYSAASKAGVDIVDVAMSAMSGNTSQPSMSSLYYALVNGPRLPEITIENAQKL
                     NHYWEDVRMYYKPFENGLNAPETEVYMHEMPGGQYSNLQQQAKAVGLGHRWDEIKQMY
                     HTVNLMFGDIVKVTPSSKVVGDMALFMVQNDLTEEDIYEKGETLSFPESVVTFFQGEL
                     GQPVGGFPKKLQKIILKGRPALNERPGLLAESVDFNEVKKELAEKIGYEPKQDEVLSY
                     LMYPQVFLDYQKAYNQFGDVTLLDTPTFFQGIRLGETINVQIERGKTLIIRLDEIGEP
                     DIEGNRVLFFNLNGQRREITIKDNSIISTVQTRLKAEPTNREQIGATMSGSVLDVLVK
                     KGDNVKKGDTLMITEAMKMETAIEARFDGEVAHVYVSSGDTISSGDLLIEVTEK"
     gene            12863..13987
                     /locus_tag="IXT20_001144"
     CDS             12863..13987
                     /locus_tag="IXT20_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4859602.1"
                     /translation="MKRLGLFWLILLLVIVAFYMEPVLFPPKLEVKENSDQQISNQMT
                     TWKYQELKATGFSTYIGKPIDQLEKDFGQAYDRLTSGFGFETRYYENKASHLTFEANI
                     QDSRVTTVKVLQTGPADIAPFYLGMTMQDLTKITTIYTNFTFEYHDTEVGIELMEEDM
                     NYRPLIAFDNDTFAILFFDQTTNGLFSVVYLDKDSLLKLLPYQVFGEGLPHYQADKSA
                     DWESINRVKERKSTTLLNMLRREDELPIYNQTLAFQNHSQLLLHDFLTRPEEILSEER
                     MADWQKSLGSAQTTVKFSLTNDELEKLISKQKLENTSGLFIHPAIDATFSFLYWYSDP
                     YNHDRFMDPGTDSVGIAFSKENMLVLLQEENGESSDSSDR"
     gene            13977..14414
                     /locus_tag="IXT20_001145"
     CDS             13977..14414
                     /locus_tag="IXT20_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HAQ4859603.1"
                     /translation="MIVNEELFQLEDQCLQVAEMIKKSKTMASYLEHKQSMEESAKVC
                     QLKEAFLSKKEAFENVAAYGEYAPDYREKKRAVRKAKRQLDMDEHVAAFRYSETQLQS
                     LLDQIVQVLAEEVSNEIKVDAGSPFFETKKHFGCGGNCHAIRQ"
     gene            14398..14736
                     /locus_tag="IXT20_001146"
     CDS             14398..14736
                     /locus_tag="IXT20_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbG family protein"
                     /protein_id="HAQ4859604.1"
                     /translation="MQLDNSEFEITKRRGLIVWVYSLKQLKNLKKFGLLHYVSRRMKY
                     VVLYLNEETLEETEERIRKLHFVRNVERSYRPDIEMNFAEKIGNKTVLKDEGFEIEEL
                     NTEIRLAEHL"
     gene            14855..15415
                     /gene="rsmD"
                     /locus_tag="IXT20_001147"
     CDS             14855..15415
                     /gene="rsmD"
                     /locus_tag="IXT20_001147"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HAQ4859605.1"
                     /translation="MRIISGNYGGRRLKSLAGANTRPTTDKVKESIFNMIGPYFDGET
                     VLDLFAGSGGLAIEAVSRGCSHAVCVDKNYQALKIIKENIEITKEPEKFTTIKLDADK
                     AIERLAAEKQQFDYLFLDPPYAKQKIIDQIHKMEELGLFSKTAVIVCETDKTVELPET
                     VAGFHQIRKQIYGITAVTIYRKEGNQ"
     gene            15412..15903
                     /gene="coaD"
                     /locus_tag="IXT20_001148"
     CDS             15412..15903
                     /gene="coaD"
                     /locus_tag="IXT20_001148"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HAQ4859606.1"
                     /translation="MKRALFPGSFDPFTKGHLDTVERAAKLFDEVVIGVFINTSKKSL
                     FPPEERMTLITKAVSHLPNVKVMHQENQLTVETAKEIGADALIRGIRSIKDFEYEREI
                     AQMNHHLYDELETVFLLAKPEYSHVSSSILKEVLHFGGDVSSYLPPVINEALAGKRES
                     NES"
     gene            15893..16936
                     /locus_tag="IXT20_001149"
     CDS             15893..16936
                     /locus_tag="IXT20_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HAQ4859607.1"
                     /translation="MKAKQWLKTVLIIIFGMLIIGFVVVPLPYYVESPGATIDLKELI
                     TVNDKEDKGSGSFSLTSVGIRQATGFLALKAKMTPFEELISEEELTGGATNEEYNQMQ
                     QYYMDSSQNAAIEQALKLADKPYKMEFKGVYVMSVEKTSNFFGKLSVGDTVTKVDGKS
                     FQSTEAFMDYVKSQKVGQTIEISYLHNGEEKTASGDLIELPTDKKAGIGITLTDHTEI
                     ESDTDVRINSGSIGGPSAGLMFTLEIYEQVTGKNLRHGKQIAGTGTINSEGEVGRIGG
                     IDKKVASADKAGVEIFFAPDDTISDDVKKEYPEIKTNYEEAKAAAKKLNSSMKIVPVK
                     TVQEALDYLEKMK"
     gene            17095..17823
                     /locus_tag="IXT20_001150"
     CDS             17095..17823
                     /locus_tag="IXT20_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016925838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComEA"
                     /protein_id="HAQ4859608.1"
                     /translation="MKGVMNDFYWSFFEKRTMIEKISKKILASPKLIIWMSMIVLIFC
                     IASGFLLSNLLFYRETEETAAKEILSSFSSSGEDPSAEGNDVEEDARPKVMYTDIKGS
                     VKEPGIYSFSSEERVYDVLKRAGGLLEEADSDRINFSAKIEDQQVLYIPAVGEELPEH
                     LDQLASSEGKQPTADSEPPKININTATPSELQQIPGIGSVKAQEIIRFREENGSFQKV
                     EDLQEISGIGEKTVEKLKNFVTIK"
     gene            17939..18184
                     /locus_tag="IXT20_001151"
     CDS             17939..18184
                     /locus_tag="IXT20_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HAQ4859609.1"
                     /translation="MIVRKETLKKPMLNVYLQNKISGIHIMNTAVSGNNSQALRERFA
                     KDVLSYTADKVFILIGTNDLAEHKQLSKETYQKICSG"
     gene            18181..18399
                     /locus_tag="IXT20_001152"
     CDS             18181..18399
                     /locus_tag="IXT20_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HAQ4859610.1"
                     /translation="MIVQLKKDYSSDHAFFITPPAVDETKQKYRNNKLIKEYISVLTE
                     VAKRHDCEVSIFIRNFLNKWIRRIFIMG"
     gene            18535..19074
                     /locus_tag="IXT20_001153"
     CDS             18535..19074
                     /locus_tag="IXT20_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComE operon protein 2"
                     /protein_id="HAQ4859611.1"
                     /translation="MLEKSSKACDTNKEKVTDKSHNERIPWDQYFMAQAVLLSLRSTC
                     TRLEVGATLVREKRIIAGGYNGAVSGDVHCIDEGCYVVDGHCLRTIHAEMNALLQCAK
                     LGIPTEGSEIYVTHFPCLACTKALLQAGVKGINYLHDYRNDPYAQALIEQLGVSVHQV
                     ALDSQHFSKLQNELTGKHV"
     gene            19067..21340
                     /locus_tag="IXT20_001154"
     CDS             19067..21340
                     /locus_tag="IXT20_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HAQ4859612.1"
                     /translation="MSSVLERHVRNRLIFPAITSMIICLLYYTDNWGYRLLLAGIFLW
                     FSLKEGRFIAYLCILCSITVSISYRLQEYSFLEEKTTSAQITVFTDTIRANGNFLTME
                     GRLSENKQKLQLSYQVNTEAELKTWLNFRGKGIELTAEGEFISPAKKRNLYNFDQEAY
                     FRNHRISGRFQIQKIEQITMQNHWRNWLQRKRGSFISFVQSEFPQKTSVYINSLLWGY
                     KDAGFREPEEIFRESGLLHLFSLSGLHIQFYLGWLYYLFRRIGWPLHVSIIPLSLLTI
                     CYVSIAGSSISVLRASLLFLLRLLVKLLNKHYSTMDLFAVVFWLLLLFDPRFLFHAGG
                     QLSFFMSFLFILISFDGDWLKKTASHVLFTMITMPLIVWHFYEWPILGSLFTLLIAPV
                     FKFLFLPGVLFLTLFNRWLPETLLLLIEEGLILFERIIDFSSGSSLIIGRLPLVLSGM
                     YIFGLLICMDRLKEQPVKHLFYVGLFSFIFLTLPFLRFSSTIAFIDVGQGDSIFLQSP
                     FRKETILIDTGGQLHFEREKWAQGMIRPPAERNIVPYLKGQGISVIDKLILTHDDTDH
                     VGELPTLSQHFTIKKIYIGWGAGRNEPLQRHLSEAQKNGTEIIEIKHGDRISGYYDLY
                     VLTPFEKGEGRNEDSIGLWMEYNNRRFLFLGDLNQAMEKQLLLIYPNLKADVVKLGHH
                     GSRTSSNTDFLSHIEAKHGIISCGVNNRYGHPHSEVLETLEENQIKTWRTDQQGMITY
                     RWHPVFHPHGLVETMID"
     gene            21378..22424
                     /gene="holA"
                     /locus_tag="IXT20_001155"
     CDS             21378..22424
                     /gene="holA"
                     /locus_tag="IXT20_001155"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HAQ4859613.1"
                     /translation="MVSNLSIQAELQKIRKEKLASCYLILGTEKFLQDQVRTEIVKKI
                     QLEGEDDLNFLSFDMENASLDEVVAEAETLPFFGDQRLVFVENPYFLTGEKVNNGIEQ
                     NTDLLTAYLKEPLESTVLVFFAPYEKLDERKKVTKQLKKTAIIINVQQLNEKEVRQYL
                     MNTLENSSIKMERSAIDLFLRLTDLDLSKMMRELQKLVLYGQNQQQITVREVEELVPK
                     TLEHNIFDMTQYILKGKTEQALRLYEDLVMQGEETIKINAILLSQLRLFLQTKFLVKI
                     GYQQANIAETLKIHPYRVKLAMQEVKKFDEHLLVQLFDQLVEMDYQIKTGQIEKELSF
                     QLFVLRTGQLLRNR"
     gene            complement(22513..22767)
                     /locus_tag="IXT20_001156"
     CDS             complement(22513..22767)
                     /locus_tag="IXT20_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HAQ4859614.1"
                     /translation="MPNIESAIKRVRTSENANVKNSSQTSAMRTAIKKFEDAVASGAD
                     NVDALYKEAVKAIDMAESKGLIHKNKANRDKSRLSKKIAK"
     gene            complement(23167..23436)
                     /locus_tag="IXT20_001157"
     CDS             complement(23167..23436)
                     /locus_tag="IXT20_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4859615.1"
                     /translation="METFEGLLIDTYVLQEQPMMLTGINLQLEDRVINGLLKDRYLAK
                     YILRLPLNKYVLEVSGKWNRRNQLVIKKMLIKNIDEYIKIIGTPE"
     gene            complement(23906..24361)
                     /locus_tag="IXT20_001158"
     CDS             complement(23906..24361)
                     /locus_tag="IXT20_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HAQ4859616.1"
                     /translation="MESWLFLGIILLVAILAKNQSLMIAAAFVLIMKLIPASRSFFPW
                     LQGKGINIGVTIITAAILIPIATGEIGLRDLLEAFKSPIGWVAVFCGGLVAVLSSKGV
                     GLIASDPQITVALVFGTILGVVFLRGIAAGPVIASGITYCILQVFALGK"
     gene            complement(24499..26427)
                     /locus_tag="IXT20_001159"
     CDS             complement(24499..26427)
                     /locus_tag="IXT20_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HAQ4859617.1"
                     /translation="MTNQKKAFAATIGCLLFMIFGFIFDRFSLTITPLFYISAIIIGG
                     YKQTSEGLKELWNDRTLNVDLLMALAAIGACLIGNYFEGAMLTFIFCLSGALEEYTTN
                     KSQKEITALMNLQPQKAQLLKENGQMQEVDVSQLKVDNLVFVAKGAAVPIDGFLESTQ
                     ATIDESALSGESVPVEKYLGDEMYAGTLNQGNPITIRVTKTSSETVFAKIIQLVEEAQ
                     NTPTQTASFIERIENNYVKLILLAVPLMILLPHFFLGWSWDESFYRGMVLLVVASPCA
                     LVASATPASLAALSNSAKNGILIKGGIHLEKLSELKAIAFDKTGTLTKGKPVVTDSFF
                     FDEKDLAQQLLVAMEQKTTHPLAHAILQYFDCTIPEEIQYLPIEEITGFGLQTTYLGA
                     QWKVGKHAYDPETMIISKEIAEMIERLENQGKTVIYLSKDQQLIAVLGLLDIPKANTQ
                     QVISYFKSQNIHTSMITGDHSGTAKAIAEQVGIEHYYASCTPEEKTQLVKKENELYQV
                     NAMVGDGVNDAPALAAASLGVAMGQGTDAAMDIADIVLMKSDLNKFVYSHRLSLKMRR
                     IIKQNIIFSILVILLLIASNFLQFLNLPLGVVGHEGSTILVILNGLRLLRPLPDLSDS
                     SKKCKSCPLYKARLSSVK"
     gene            complement(26624..27457)
                     /locus_tag="IXT20_001160"
     CDS             complement(26624..27457)
                     /locus_tag="IXT20_001160"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HAQ4859618.1"
                     /translation="MFFANIIKAVILGIIEGITEWLPISSTGHLILADEFIKLDASSQ
                     FMSMFNVVIQLGAILAVVVLYFHKLNPFSPSKSTVEKKDTWTLWFKVIVACIPAIIIG
                     LPLDDWLEANFHKFLPVAIVLIVYGIAFIIVEKRNKNKTPRWTSLNDFTYQAALLVGL
                     FQVLALVPGTSRSGATILGSILIGASRFVATEFSFFLGIPVMFGASGLKIVKYLAEGN
                     SFKMEETVVLLVGTFVSFVVSVLAIKFLLSYLKKNDFTAFGWYRIILGIILIGYWFIA
                     M"
     gene            complement(27649..28353)
                     /locus_tag="IXT20_001161"
     CDS             complement(27649..28353)
                     /locus_tag="IXT20_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HAQ4859619.1"
                     /translation="MTETTLYIVRHGKTMFNTIERVQGWCDTPLTKQGQEGIHYLGRG
                     LKNVDFSFAYSSDSGRAIETARIILGEHSKGKEIPYFIEKRIREWCFGSLEGGYDMEM
                     WGVIPRVLNFKTYDEMFTTEVSFEQIANAVYNLDTAGWAETYETLRDRVWTGFEDIAH
                     HCEKNGGGNVLVVSHGLTIAFLLNLIDPKQPVRAGLLNGSVTKVTYANGRFSIHGIND
                     TSYIDNGKTHSRDSFL"
     gene            complement(28376..29002)
                     /locus_tag="IXT20_001162"
     CDS             complement(28376..29002)
                     /locus_tag="IXT20_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HAQ4859620.1"
                     /translation="MHIERLKTGMIEENCYLVYNEEALLIIDPGEDAERIKQEIKKTN
                     QQPVAILLTHTHYDHIGAVEPLRAYYQIPVYVSPLEQKWLGDPILNLSGLGRHDDIAD
                     VIVSPAEYEFEMKTYTLGNMSFRVVPTPGHSIGSVSFIFDDFVVSGDALFRGSIGRTD
                     LYTGNLEQLLHSIRTQLFVLPDEFAVYPGHGDATTIEQEKRTNPFFNR"
     gene            29328..31703
                     /locus_tag="IXT20_001163"
     CDS             29328..31703
                     /locus_tag="IXT20_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HAQ4859621.1"
                     /translation="MIKQSKISVRHLVEFILRRGSIDNRKKSNHTALEGARIHRKLQK
                     DAGENYEKEVFLKTTVQLDEYYLIVEGRADGIFKRDGIYYIDEIKTSEPRFEDLEPEQ
                     VELFFHQARVYAYIYSQEHDLQEINLQLTYFQTAEEVITRKVEHQTREQLETFFKKLT
                     EDYKEWLVFQENWRTVRNASLMALKFPHDEYRKGQRELAVAAYKTIRTKQKLFVEAPT
                     GTGKTISTLFPALKAVGEEEGEKIFYLTAKTITRQVAEDAMTALKDTGAEVKSVTLTA
                     KDKICFLDETTCNPDQCPYANGYYNRINEGLWDLLNHENQITREVIETYARKHTLCPF
                     ELSLDVSIWCDVIIGDYNYLFDPTVYLRRFFEDEKNEDYLFLIDEAHNLVNRSREMYS
                     AELSYEKTKRSKEAIPKEFKKLHRRFNKLLKEFDSIREIAKEDHWDYHHQKAPAESLV
                     KAGYQLSEKIKEWLAEFPEHPSQEQLLPYYFDLLHFLKVSEFYDDHYETTVEKTYRDL
                     IVKEFCIDPSLFLEQSLDKGKSSILFSASFSPLSYYQETLGGQKSLAYKLPSPFPEGN
                     QQVFIANYLETTYRKREHSLAKLVETIHAFADGKVGNYFVFFPSYQYLDLAVEAFRQQ
                     YPDSNVLIQETDMNEEERERFLTKFQTNPAETLIGFCVLGGIFSEGIDLRGDRLIGSM
                     IVGVGLPQMNHEQELIKSYYDEKEHLGFAYAYQLPGMNKVLQAGGRVIRGMADQGIVV
                     LADQRFSSFSYRRLFPQHWQTAREVRSVGELEEGIQRFWLSKEAVNQKNEE"
     gene            complement(31727..32218)
                     /locus_tag="IXT20_001164"
     CDS             complement(31727..32218)
                     /locus_tag="IXT20_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HAQ4859622.1"
                     /translation="MAKDASFDIVSEVNTEEVKNAIQQTTKELSNRFDFKDSTVEIKL
                     ENSNLVVLSDDDFKIEQIKDVLFGKLIKRNVPIKNIHFSESEHALGGKARQTADLVNG
                     IDRENAKKITTAIKNEKLKVKAQIQEDQIRVTGKNRDDLQAVIALLRKLDLPIELQFT
                     NYR"
     gene            32405..33325
                     /locus_tag="IXT20_001165"
     CDS             32405..33325
                     /locus_tag="IXT20_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAQ4859623.1"
                     /translation="MTAYHKNERLKKIVVILITGLTAAVALNYFLIPANVFSAGMNGI
                     AQIISSLLYEWFHIKIDTGTFIFLLNIPVFVLGFLKVGKRATILSFVNVVCVSVMTML
                     LPVGQITDNILMNALVGGVLLGLGVGLSLKMGFTTGGMDIISLVLSQTTGKTVGNYML
                     ILNGIIVVIAGFFFNWESALYTIISIYAMTQVIDAIHTSHQKVTAMIVTVKPEEVTEE
                     LAKRMVRGMTLLPSVGGYSKKQGNMIMMVITRYEMYDLDQIVHEIDDDAFINILPTHS
                     VFGRFANENEQKMYRTTGVLPEIKNRSQKK"
     gene            33479..33823
                     /locus_tag="IXT20_001166"
     CDS             33479..33823
                     /locus_tag="IXT20_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HAQ4859624.1"
                     /translation="MSEANQERSGQEIELIKERILAALEMVIDPELGIDIVNLGLIYD
                     IEFNPENGETVIKMTLTTMGCPLADILTESIHDALKEVPEVTKAEVKLVWYPAWTTDK
                     MSRYARIALGIR"
     gene            complement(34013..34513)
                     /locus_tag="IXT20_001167"
     CDS             complement(34013..34513)
                     /locus_tag="IXT20_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ4859625.1"
                     /translation="MKLIKLWEADLKKAYQLQTSFGPNESGFINNAYGYTFAEFLDYV
                     KKCEKTSLGIDLPESYVPATVFILESDEHEYVGIFNLRHKLNETLRNGAGHIGYGISP
                     NYRKKGYATQGLALVLQEAKKIGIDEALLSVNKKNLGSLKAQQKNGAAIYREDEKEYY
                     TKIKLM"
     gene            35041..35124
                     /locus_tag="IXT20_001168"
     tRNA            35041..35124
                     /locus_tag="IXT20_001168"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:35075..35077,aa:Leu,seq:cag)
     gene            complement(35448..>35882)
                     /locus_tag="IXT20_001169"
     CDS             complement(35448..>35882)
                     /locus_tag="IXT20_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HAQ4859626.1"
                     /translation="YSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEPVQAEQPVVQET
                     VQTETQAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTATNGRYIGRYQL
                     DSSYLNGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
BASE COUNT        11868 a         6262 c         7405 g        10347 t
ORIGIN      
        1 ccgtcacctg tcacagggac agttaaaaac gtgattgttc cagaaggcac tgtagcaaat
       61 gttggggatg tattagtaga aatcgatgct cctggacaca atagtgcacc gtcaactagt
      121 gcgccatcag ctgaagcacc aaaagaaaaa gtggaaacat caggttcagc aagtgtcgtt
      181 gaagctgccg atccaaataa acgtgtattg gctatgccat ctgttcgtca atttgcccgt
      241 gaaaaagatg tagatatctc acaagtaact gcaactggaa aaggtggcag agtaacaaaa
      301 gaggacatcg aaaacttctt ggcaggtggt ccaagcagtg caccagcgaa atcagaagcg
      361 ccagaagcgg cagcaccgaa agaagcagct cctgcagcag aaagcaaacc agctgcacca
      421 gcaaaaccat tcaagtccaa cttaggagac ttggaagaac gtgtagcaat gacgccaaca
      481 cgtaaagcta tcgctaaagc aatggtcaac agtaagcata cagctccaca cgtgacattg
      541 catgatgaag tagaagtgtc taaactatgg gataatcgga aacgatttaa agaagttgct
      601 gctgcaaatg gtacgaaact tactttcttg ccatatgtcg tgaaagcttt aacagcgact
      661 gttaagaaat acccagtatt gaatgcatct atcgacgatg caaaccaaga aatcgtttat
      721 aaacattact acaatatcgg tattgcaact gatacggatc acggactata tgtaccaaat
      781 gtgaaagatg cagaccgcaa aggaatgttt gctattgcag acgaaatcaa tgagaaagca
      841 aaattagctc atgacggcaa actatctgca gaagatatgc gcaatggaac aattacgatc
      901 agcaatatcg gatctgtagg tggcggttgg tttacaccag tcatcaacta tccagaagta
      961 gctattttag gtgtaggaac gattgcacaa caaccaatcg taaatgctga aggcgaaatc
     1021 gttgtcggtc gtgtgatgaa actgtcatta agtttcgatc accgtatcgt agatggagca
     1081 actgcgcaac aagcaatgaa taatatcaaa cgtcttttag ctgatccaga gctattaatg
     1141 atggaaggat gataaacaaa tggtagttgg agattttgct gtagaattag atacagttgt
     1201 aattggtgcg ggacctggtg gctatgtagc tgccatccgc gctgctgaaa tgggacaaaa
     1261 agtagcaatc attgaaagag agtatattgg cggtgtttgt ttgaatgtcg gctgtattcc
     1321 ttcaaaagca ctgatagctg caggacacca ttatcaagag tcactagatt ctgaaatgtt
     1381 tggagtaact agtgaaaacg tcaaactaga ttttgcaaaa acacaagaat ggaaagaaaa
     1441 caaagttgtc catacgttga cttctggtgt tggtttccta ttaaaaaaac ataaggttga
     1501 aacaatcgaa ggagaagcgt tcttcgtaga tgatcacaca ctacgcgtca ttcatccaga
     1561 ttcagctcaa acttattcgt tcaataacgc aatcatcgca acaggttccc gtccaatcga
     1621 gattccagga tttaaattcg gaggacgtgt gttagattct actggcggat tagcattgaa
     1681 agaagtacct aagaaatttg tgatcatcgg tggcggtgtg attggtgccg aattgggcgg
     1741 agcgtacgca aacttaggag cagaagtaac gattcttgaa ggctcaccac aaattttacc
     1801 aacttacgaa aaagatctag taaaattagt agaagatgat ttcaagaaaa aaggcgtaac
     1861 agtcgtaacg aatgcaatgg cgaaagaagc agtcgataac ggtgacagcg tgacagtgaa
     1921 atatgcagtg gacgggaaag aagaatccgt tactgctgat tatgtgatgg tcactgtagg
     1981 tcgtcgtccg aatacagatg atatgggctt agaacaagcg ggtgtcgaag taggagaacg
     2041 aggattgatc acggtggata aacaaggacg tacgaacgta cctaacatct atgccatcgg
     2101 tgatatcgtt ccaggggcgg cattagcaca caaagccagt tatgaagcaa aaatcgctgc
     2161 tgaagcaatc tctggcaaaa aagtagctgt agattataaa gcaatgccgg cagtagcctt
     2221 cactgatcct gaattggctt ctgtaggtat gacgatcaaa gaagcaaaag atgctggtat
     2281 cgaagcaaaa gcatataaat tcccattttc agggaacggt cgggcattat cgcttggtaa
     2341 aacagaagga ttcatccgtc tagttacgac gatcgaagat aacgtgctta ttggcgcgca
     2401 aatcggtgga gtcggcgcaa gtgatatggt ctctgaatta gctttagcaa tcgaatcagg
     2461 aatgaatgct gaagatatcg ccttgacgat tcatcctcat ccatctcttg gcgaaatcgt
     2521 catggatgct tcggaattag cattaggttt accaatccat atttgatttg aataatacag
     2581 gcgaaacgca actgaaaaat cagttgcgtt ttgtttttta gaattatcaa taatattatt
     2641 atgtaaactt aaaaatatga caacttcttg gtttattacc acttcctatg ctacactgaa
     2701 gaggaatatg aaataggaga gttgaagatg caagaatatc agtatccact tgatttagat
     2761 tggacaacag acgaaatggt cattgtaatg aagatgtggg aagatctgga acgtgcgaat
     2821 gaaaaaggga taaacaatga agcttttttg aagacttatc agcagtttaa aactgttgtc
     2881 aaatccattg gtgaggaaaa acgattggga cgggaatttg aaaaagcttc cggttattct
     2941 ttatatcgta cagtgcagca agcaaaaaaa aatccaaaca aaactttgag aatgaacggg
     3001 tgagataaat ggaacagatg attgatcata tcaagtcctg gcttgaagaa gctggtgaga
     3061 aaatcaaatc ttctcaacaa acattaacaa tctcgcaaaa atcaaatcga acggatctag
     3121 taacaaatgt cgatcgaaac atccagaatt ttctaatcga aaaaatccat tcctttgatc
     3181 cagaggcgaa aatattggca gaagaagaag ggtgtaatca attatcggat ctcgatggaa
     3241 gagtctttat catcgatcct attgatggca ccttgaattt tgtgattgaa ggggaaaatt
     3301 tttgtattat gctcgctgtt tatgaatccg gcgttggaaa gcttggtttc atttatgacg
     3361 tgatgagaaa tgaacttttt tggggtggaa aaggaatagg cgtgtataaa aatgatgagc
     3421 cattgcctag accagcaaat aaatccttat cggaaggctt acttggtgtg aatagttatc
     3481 ttttcggtca taataaatat ggaatccgag aaattgcgga aagcagcatg gggacacgta
     3541 tgtacggatg cgctggtctt gaactagtgg caatcttgaa aggaaaccac attgcttata
     3601 tttcaaacct tagcccttgg gattatgcag cgggaaatgt cttattggaa gagtttggta
     3661 tggtaacaag cggtcttttt ggtactcctt tgacatttga aaaaagggaa tactatttag
     3721 cagggacttc ttgtgcatat gcagaaatcg cagacctttt gaaaaaagaa ggctgaaaaa
     3781 aaggtagaaa tcattcacaa aaatgataaa aacgagcaaa agattaattt tctcgaaaaa
     3841 atgaaaacat tttactatat ttcatgaaaa aagtctgcta taattagcag tcgagtaaaa
     3901 tttgccctgt cataaagaac caataaaaaa ggagttagtt acattgaatt atagaaacga
     3961 tatccgtaac gtcgcaatta ttgcccacgt tgaccacgga aaaacaacat tagttgatga
     4021 actattaaaa caatcagata cattagacgc acatacgcaa ttgcaagaac gtgcgatgga
     4081 ctcaaatgcg ctagaaaaag aacgtggtat cacgatttta gccaaaaaca cagctgtaga
     4141 ctataaaggt attcgcgtaa acatcatgga tactccagga cacgcggact tcggtggcga
     4201 agtagaacgg atcatgaaaa tggttgacgg cgttgtgttg gtcgtagatg cgtatgaagg
     4261 aacaatgcct caaacacgtt ttgtattgaa aaaagcactg gaacaacata tcacaccaat
     4321 cgttgtcgta aacaagattg acaaaccttc tgctcgtcct gagcatgtcg tagatgaagt
     4381 tcttgaacta ttcatcgaat taggagcaga tgatgatcag ttagactttc ctgtgattta
     4441 tgcatctgca ctaaatggta cgtcaagctt gtcagatgat ccagcagatc aagaaccaac
     4501 catggcacct atttttgata caattattga aaaaatccct gctcctgtcg ataacagcga
     4561 cgaaccatta caattccaag tttcattact tgactacaac gattatgtag gtcgtatcgg
     4621 aatcggacgt gtatttagag gaacaatcaa agtaggagac caagtagcat taattaaatt
     4681 agacggaaca gttaagaaat tccgtgtaac gaaattattt ggtttctttg gactaaaacg
     4741 tctagagatt caagaagcaa aagctggaga cttgattgct gtctcaggta tggaagatat
     4801 ctttgtcggt gaaacagtta ctcctgtgga tcatcaagat gcgttaccta tccttcacat
     4861 tgacgaacca acattgcaaa tgactttctt agttaataat tcaccatttg ccggtcgaga
     4921 aggtaaattc gtaaccgctc gtaagattga agaacgtttg atggcagaac ttcaaacaga
     4981 tgtatcctta cgagttgaac caacaaactc tccagatgca tggacagttt ctggtcgtgg
     5041 agaattgcat ttgtctatct tgattgaaaa catgcgccgt gagggatacg aattgcaagt
     5101 atctcgtcca gaagttattg aaaaagaaat cgacggtgta aaatgcgaac cgtttgaacg
     5161 cgtgcaaatc gatacaccag aagaatatat gggtagtgtt atcgagtcct tgagcttacg
     5221 taaaggtgaa atgcaagata tggtccatac aggaaacgga caaatccgtt tgacattctt
     5281 aacacctgcc cgcggtttga ttggttattc aactgaattc ctttcaatga cacgtggtta
     5341 cggaatcatg aaccatactt ttgaccaata tctgccaatg cttcctggtc aaattggcgg
     5401 acgtcaccaa ggggcgcttg tttcgatcga tacaggaaaa gcaacgactt attctatcat
     5461 gagtatcgaa gaacgcggta cagtcttcgt agaaccaggt acagaagtat atgaaggaat
     5521 gattatcggg gaaaacagcc gtgataatga tttgactgtc aacatcacga aagcaaaaca
     5581 aatgacaaac gtacggtctg ctactaaaga ccaaacatct gtcatcaaaa aaccaaaaca
     5641 attgacgttg gaagaatctt tggaattctt gaatgatgat gaatattgcg aagtaacacc
     5701 agaaagcatt cgcttgagaa aacaaatttt agagaaaaat gctcgtgaaa aagcatctaa
     5761 aaagaaaaaa taatctgtaa aaggatgaag agagaaacca gcttgttata aaaaatagct
     5821 ggtttctttt tttgtccaac tcttttctgt ggagaagtca tctttgtcaa tggacataaa
     5881 ttcagaaaat acgccgaaag acgtttaaaa gacttttaaa aaacttattt ctagagagtt
     5941 tttgtttcat aataagataa tgtttcacga aaggagatca acttgtcctt cgaagaaaat
     6001 cgaagtaaaa tggcaagaga aaggagcggg aagatgaacg acttattaaa acaatacttt
     6061 ggctatgatg agtttagacc tggtcaaaaa gaaattatcc agaaagtcat tgatcaggaa
     6121 aatgtattag gaatcatgcc taccggaagt gggaaatcaa tctgttacca gcttccagct
     6181 ttactattag atgggcttac tgtggttgtg tcccctttaa tctctttgat gaaggatcag
     6241 gtggacgcgg cgaatcaatt aggtataccc gccactttta tcaatagctc gttagatggt
     6301 tacgaaacag ctagacgctt ccaagaaatc gatagacagc agtaccgttt actttatatc
     6361 gctcctgaac gttttatcat gccggatttt atccaagcaa tgaagcgatg gaacgtccgt
     6421 atgatcgcta ttgacgaggc acattgtatc tctcagtggg ggcatgattt ccgcccaagt
     6481 tatttacaga tggcgaacca gctagatcaa ctgccaaatc gtcctgtaat tgttgctttg
     6541 acagctacag cgactgtgca agtagctgct gacataaaaa gacttttaaa aattccagaa
     6601 aacaaccata ttcagactgg ttttgaacga gaaaatctac gatttcaagt aattaaagat
     6661 caaaaaaaag aacaatatct aatcgaatat ttaaagatca acaaaaatca atctggtatc
     6721 atttatgcag caacgagaaa agaagtagac cgtctctatc atttgcttaa aaaattcgat
     6781 ttttccgtgg gtagatatca tggtggtcta aatgagaatg agcgaacaga aatgcaagaa
     6841 gcctttttat acgatcgctt gcagctgatt gtagcgacaa atgcatttgg tatgggtata
     6901 aataaaagca atgtccgttt tgttatccat tatcaaattc ctggctctct agaagcatac
     6961 tatcaggaag ctggtcgggc gggaagagat ggtctttcca gtgaagccat cctgcttttc
     7021 tctcctcaag atatccaagt ccaaaagttt tttatccagc agtcccaaag agaagaaggg
     7081 caaaagcaaa aagaatatga aaaattaaaa gcgatgacag aatacgtgta tattgagtcc
     7141 tgtcttcagc aatacatcct aaactatttt ggcgagacaa gcagtccgtg caaccgttgt
     7201 ggtaactgtt tagatgacag agaaatagta gaagtaacga cagaagcaca gatggttctt
     7261 tcttgcttaa aacgtatggg tgagaactat ggtaaacaaa tgctgatgaa agtcttagca
     7321 ggatcgaaag aacaaaagct gcaagcttta ggttttggac atttatctac ctatggactg
     7381 atgaaaaacc aatcgcaaaa agaaacgatg cagttaattg aatatttgat atcgaatggc
     7441 tatcttctga ccatcaatgg tgagtatcct gttttgaaag taacagaaag agggatacaa
     7501 gtattgaaag gacaagaatc tgtttatcga aaggaaccca agaaagtcca gcagctatcg
     7561 gatgaagaaa ccgatacact atttgaagtg ctgcgggaac tgcgaacgga tctagcatca
     7621 gaagcaggcg ttcccccgta tgtcgtattt tcagattcca cattgaaaga aatgagccga
     7681 atacgtccat caagcagatt ggagatgctt cagataaaag gagttggcca aagcaagctt
     7741 gataaatatg gcgaagcctt tttatctcgg ataaaaaatg cggactctaa cgttctggat
     7801 aagtagaaag aacgaatacg taaaaatgaa taaaaacaac tggttaaatc tgtttttttt
     7861 gctccttatg aaaatcaaat atgttataat taaggaatta gaatgggagg taagcacaga
     7921 tggattcttt gtcgaaaaaa cttgcgatcc aagtattaaa tgatgaagca gaacggatca
     7981 aacgattaat ccgaaatcaa aacaacagtc tctgtatctc acaatgcaaa gccttcgaag
     8041 aagtggtaga tacacagatg tacggctttt cccggcaagt cggttttgct gtccgtatag
     8101 gagtaatcgg aaagaacgag ggccagctga tgttaagtga gctagaacga gaattgaatc
     8161 agttatatag tgaggtttac caagaaattt atgataaaaa agaagttggc aaggaggaat
     8221 agacttgcct aagaaagtga aaaaaaggca tctattagac tatagcatct tgattcccta
     8281 tttaatttta tgtgtattgg gattgatcat ggtatatagt tctacgtcgt atctattatt
     8341 agaaaacgga caaaatccgt ccgcatcagt cattaaccag agtatttttt gggtgcttag
     8401 cttgattgct atcgcactac tttataaaat gaaaacagat gttttgaaaa accagcgttt
     8461 gatcatggca gccatcgcag ttttgacgat tttgctgctg attgttgtgt tttttggtaa
     8521 ggagatcaat ggggcgaaag gttggctgca aattgcagga ttctcgatcc aacctgccga
     8581 atacctaaaa atcatttcta tctggtacct gtctctgact ttatctaaaa gacaaaacag
     8641 tgtacaaaaa gattttcttg ggactgtgaa acgtccactt gcaatggtta tcggacttac
     8701 tgcattagtc gctattcttc ccgattttgg taatgcggca gttatctttt tgattatttt
     8761 ggttctgctt ttagccagcg gagtcaatta tgtgtatact ttgattgtag gagtaggagg
     8821 cttttgtcta agtactttta cgatctggct gatcaatatc acaaacggaa agatcttacc
     8881 cggacgttta cagtatatct ataatcgatt tgctatttac caaaatcctt tctcagacga
     8941 attaaataaa gggcatcaac tagtcaacgg ctactatgcc atgttcaacg gaggactatt
     9001 tggcagggga ttaggtaaca gtatccagaa aaaaggattt ttacaagaag cacaaaccga
     9061 ttttatttat gcaatcgtag tggaagaact gggagtgatt atggggattt tgatattagc
     9121 gttgctattc tttatgattg tgcgcattgt ccttgtaggt atccgttcaa aagatccgtt
     9181 caattcgctt ttgtgcattg ggatcggcgc aatgtttttg atccaagtat ttgtcaatct
     9241 tggaggaatt actggcgtta ttcctttgac agggatcacg ttcccatttt taagtcaagg
     9301 aggctccagt ttgctgatgc tttcgatttg tgtcggcttt gtcctcaaca tcagtgcgga
     9361 tgaaaaacgc aagagcttag gtttataata aaaaaagatg taggagtggt tcgatgaaaa
     9421 aagttttagt tgctaacagg ggtgaaatcg ctgtacgtgt atttcgtgct tgtacagaat
     9481 taggaattaa aactgttgga atctatgcag aagaagatga atattccgta caccgtttca
     9541 aagcggatga agcatattta gtaggacaag gaaaaaaacc gattgatgcg tatctagata
     9601 ttgaagggat catttctatc gcgaaagaat gcggagctga cgctattcat ccgggttacg
     9661 gtcttttgtc ggaaaacttg aattttgcgc aacggtgcga agaagaaggc atcatttttg
     9721 ttggacccaa actgcatcat ttagatattt ttggagataa aatcaaagca aaagctgcag
     9781 ctatcgaagc ggggattgct tcgattccag gcacagatgg accaatcgct tctatagacg
     9841 atgccttgga gtttgcgaaa caatacggtt atccgattat gatcaaggca gctctaggcg
     9901 gcggcggacg cggcatgcgt gtagctcacg atgaaaagag tgcaagagag ggctacgaac
     9961 gtgcgaaaag cgaagcaaaa gcagcctttg gtagtgatga agtgtatgtt gaaaaataca
    10021 tcgctaatcc taagcatatc gaagtacaga ttctcgggga tacgcatgga aacgtcatcc
    10081 atctttttga aagagattgt tctgttcagc gccgtcacca aaaagtagta gaagtagcgc
    10141 cttgtgtctc tatgaatgaa cagcaacgtc aaaaaatctg ccaggcagct gtccaattga
    10201 tgaaatatgt cgggtatgtc aacgcaggta cagtagaatt tcttgttgaa ggagatgatt
    10261 tttactttat cgaagtcaac cctcgcgtcc aagtggaaca tacgatcaca gaaatgatta
    10321 cagatattga tatcgtaaca acgcaactgt tgattgctca aggtttggat ctgcataaag
    10381 aaatcggatt gcctcagcaa gaaggaatta aattaaatgg ttcagccatt caatgccgaa
    10441 tcactacaga agacccgttg aataactttt taccagacac aggggaaatc gatacgtatc
    10501 gttcacctgg tggttttggc gtacgtctgg atgtcggaaa tgcttacgcg ggctatgtag
    10561 tgacaccgta ttttgattca ttgctagtaa aagtatgtac gcatggtgct acctttgaaa
    10621 cagccattca gaaaatggaa cgttgcttga aagaatttag aatccgtgga gtgaaaacaa
    10681 acattccatt tatgctcaat gtcatcacac atcctgaatt ccaatcagga aatgcaaaaa
    10741 caaccttcat tgatagtaca gcaacattat ttgaatttcc tcgactacga gaccgcggaa
    10801 acaaaacgat gaaatatatc ggtgaaatta ctgtgaatgg ttttccaggt atcgaaagtg
    10861 gcgaaaaacc attttatgaa gaaccacgta tgcctaaaga tctaatcaca agaactgatt
    10921 acgtaacggc taagaatgta ttagatgcga agggtgccga tgctttggtc gaatggatca
    10981 aaggacagga aaatttgttg ttgacggata cgacatttag agacgcccat caaagtctgc
    11041 tagcaactcg tgttcggaca aaggatttca agcagattgc acgtttgact ggagaaggtc
    11101 ttcctgaact cttttcaagt gagatgtggg ggggagcaac atttgatgtg gcttaccgct
    11161 ttttgaatga agatccttgg caacggttga gaaaaattcg ttctttaatg cctaatacgt
    11221 tgctgcaaat gcttttcaga ggttcaaatg ccgttgggta ttctaattat cctgacaatg
    11281 tattggtgga atttgtcaaa gaagcagcag cgcaaggaat cgatgtattt cgaatctttg
    11341 atagtttgaa ctggactcca caaatggaaa aaagtatcca agctgtacgg gatactggta
    11401 aaatcgcaga ggcagctatt tgctatactg gcgatatcaa cgatccaagt cgggcaaaat
    11461 acaatgttca gtattataaa gacatggcaa aggaactaga acaactgggt gcgcatatca
    11521 ttgcaatcaa agatatggct gggctgctta aaccgcaggc agcttatcgt ttgattagtg
    11581 aattgaaaga aacgacagat ctgccaatcc atctgcatac tcatgatacc agcgggaatg
    11641 gaatcatcac ttattctgct gcttcaaaag caggagtaga catcgttgac gtggcaatga
    11701 gtgcaatgag tgggaatact agtcagccta gcatgagcag cttgtattat gcactggtca
    11761 atggtccacg gctgccagaa atcacgattg aaaatgcaca gaaattaaac cattattggg
    11821 aagatgtgcg catgtattac aaaccgtttg aaaacggctt gaatgctcct gaaacagaag
    11881 tatatatgca tgaaatgcca ggaggacagt attcgaatct acagcaacag gcaaaagctg
    11941 tcggattagg tcatcgctgg gatgaaatca aacaaatgta tcatactgtc aacttgatgt
    12001 ttggcgatat cgttaaagta actccatcat ctaaagtagt tggagatatg gcactattta
    12061 tggtccaaaa tgatcttaca gaagaagata tttatgaaaa aggcgaaaca ctaagtttcc
    12121 ctgaatctgt tgtgactttc ttccaaggag aactagggca acccgttggc ggatttccta
    12181 aaaaattgca aaagattatt cttaaaggca gacctgcatt gaatgaacgt ccaggtcttt
    12241 tggctgaatc agtcgacttc aacgaagtaa aaaaagaatt ggcagaaaaa attggctatg
    12301 aaccaaaaca agacgaagta ttaagctatt tgatgtatcc gcaagtcttt ttggactatc
    12361 aaaaagcata taatcaattt ggcgatgtta cattactgga cacaccaaca tttttccaag
    12421 gtatacgttt gggtgaaaca atcaatgtac aaatcgaacg cggaaaaacg cttatcattc
    12481 gtttagatga aatcggagaa cccgatatcg aagggaatcg cgtactgttc ttcaatttga
    12541 atggacaaag acgtgaaatc actatcaaag ataattcgat catcagtacg gtccaaaccc
    12601 gattgaaagc tgaaccgaca aaccgagaac aaattggagc aacgatgtct ggttctgttt
    12661 tggatgtatt agtgaaaaaa ggcgataacg ttaaaaaagg tgatacgttg atgattactg
    12721 aagcgatgaa aatggaaacg gccatcgaag cacgttttga cggagaagtc gcacatgtat
    12781 atgtgtcttc aggagacact atttcgtctg gtgacctttt gattgaagtg actgagaaat
    12841 aataagcgaa aggtggggaa acatgaaaag actgggtttg ttttggctga ttctgctatt
    12901 agttattgtg gcattctaca tggaaccagt cttgtttccg ccaaaacttg aagtgaagga
    12961 aaactctgat caacaaatat caaaccaaat gacaacgtgg aaataccagg agcttaaagc
    13021 aactggtttt tctacttata tcggaaaacc gatcgatcag ctggaaaagg attttggcca
    13081 agcctatgat cgattaacta gcggatttgg ttttgaaaca agatattatg aaaataaagc
    13141 tagtcatctc acatttgaag cgaacatcca agacagcaga gtcacaactg ttaaagtact
    13201 gcaaacaggg cctgctgata tcgcgccgtt ttatttaggg atgacgatgc aagatcttac
    13261 gaaaatcact actatctata ctaatttcac ttttgagtac catgatacag aagttggaat
    13321 cgaactaatg gaagaggata tgaattatcg tccgctaatt gcttttgata acgacacttt
    13381 tgctatttta ttttttgatc aaacaacaaa cggcctattt tcagttgtgt atttagataa
    13441 agatagtctt ttgaaactat tgccatatca agtgtttggt gaaggccttc cacattatca
    13501 agcagataaa tcggctgatt gggaatcaat caatcgcgtt aaagaacgaa aaagtacgac
    13561 tttattaaat atgcttagaa gagaagacga acttcccata tacaatcaaa cacttgcctt
    13621 tcaaaaccac agtcagctgc tactacatga ttttttgaca aggcctgagg aaatattgtc
    13681 agaggaacgt atggctgatt ggcaaaaatc gctaggcagt gctcaaacga cagtgaaatt
    13741 ctcactgacg aatgatgaat tggagaagtt aatcagtaaa caaaaattag aaaatactag
    13801 tgggctattt attcatccgg cgattgacgc tactttttcg tttttatatt ggtacagcga
    13861 tccttacaac catgaccgtt tcatggaccc gggaacggat tctgtaggca ttgctttttc
    13921 taaagaaaat atgttagtct tattgcaaga agaaaacgga gaaagtagtg atagcagtga
    13981 tcgttaacga agagctgttt caactggagg atcaatgcct ccaagtcgct gaaatgataa
    14041 aaaagagtaa aacgatggct tcttatcttg aacataaaca gtcaatggaa gaatctgcaa
    14101 aagtttgcca gttgaaagaa gcgttcttgt ctaagaaaga agcatttgaa aatgtagcgg
    14161 catatggtga gtatgcacct gattatcgtg aaaaaaaacg agctgttcga aaagcaaaaa
    14221 gacagcttga tatggatgag catgttgcag ctttccgata tagtgaaaca cagctgcaaa
    14281 gtctgttgga tcaaattgtc caagtacttg ccgaagaagt ttctaatgaa atcaaagtag
    14341 atgcaggtag cccgtttttt gaaacgaaaa aacactttgg atgcggaggt aattgtcatg
    14401 caattagaca atagtgaatt tgaaataaca aaacgacgcg ggctgatcgt atgggtctac
    14461 agcttaaaac aattaaagaa tctgaaaaag ttcggcttgt tgcattatgt ctcaagaaga
    14521 atgaaatatg tcgtgcttta cttaaatgaa gagacattgg aagaaacaga agaacgaatc
    14581 cgtaaattac attttgttcg aaacgtagaa cgttcttacc gaccggatat cgaaatgaat
    14641 tttgccgaaa aaataggaaa caaaacagta ttaaaagacg aaggtttcga aattgaagag
    14701 ctaaatacag aaatccgatt agctgaacat ctttaaaaat ctcttatgag cagtttttaa
    14761 ataaaaaata acttggataa cgatcgattt ttgatcattg tccaagtatt tttttggtat
    14821 actagtacca gtaccatttc taaggagtga gtagatgcgg ataatatcag gaaattatgg
    14881 cgggcgccgt ttaaaaagtt tagcaggggc gaacaccaga ccaactacag ataaagtaaa
    14941 agaatccata ttcaatatga ttggaccata ttttgatgga gagacagtat tggatctgtt
    15001 tgcaggaagc ggtggtttag ctatcgaagc agtatctaga ggctgcagtc atgcggtatg
    15061 cgtagacaaa aattaccaag cgctaaaaat catcaaagaa aacatcgaaa ttacgaaaga
    15121 accagagaaa tttaccacaa taaaacttga tgcggacaaa gcaatcgaac ggctagcagc
    15181 ggagaaacaa caattcgact acttgtttct tgatccgcca tatgcaaaac aaaaaataat
    15241 cgatcaaatc cataaaatgg aagaactggg acttttttct aaaaccgcag ttatcgtatg
    15301 cgaaacggat aaaacagtcg aattgccaga aactgtggct ggattccatc aaataagaaa
    15361 acagatttat ggcataacgg ccgtaactat ctatagaaaa gagggaaatc aatgaaacgt
    15421 gcattattcc caggaagttt tgatcctttt acaaaaggtc acttagatac ggtggaacgt
    15481 gcggctaaat tattcgatga ggtagtaatt ggtgttttta tcaatacttc aaagaaaagt
    15541 ttgtttcccc cagaagaaag aatgacattg atcacaaaag ctgtttctca tcttcctaat
    15601 gtaaaagtca tgcatcaaga aaatcaattg acggtagaaa cagcaaaaga gattggtgca
    15661 gatgcgttga tcagaggcat acgaagtatc aaagactttg aatatgaacg agagattgct
    15721 cagatgaatc atcatctata tgatgaattg gaaactgtct tcttattagc aaaaccagaa
    15781 tatagtcacg tgagttccag tattttaaaa gaagtactgc actttggcgg agatgtgagc
    15841 agctacttac cgccagtgat caacgaagct ttagctggaa agcgtgaatc aaatgaaagc
    15901 taaacaatgg ttgaaaacag tactgattat catatttgga atgttgatca ttggatttgt
    15961 agtagttccc ttgccgtatt atgtagaatc accgggagcc acgatcgatc tgaaagaact
    16021 aattacggtc aatgataagg aagataaagg ttctgggtct ttctctctta cctctgttgg
    16081 tatcaggcaa gctacaggtt tcttagcttt aaaagctaaa atgacccctt ttgaagaatt
    16141 gatcagtgaa gaagaattga ctggtggggc gacaaatgaa gaatataatc aaatgcaaca
    16201 gtattatatg gattcttccc aaaatgcagc aatcgaacaa gcgttaaaac ttgcagacaa
    16261 gccttataaa atggaattca aaggcgtata tgtcatgagt gtagagaaaa catcgaattt
    16321 ctttggcaag ctttcagttg gagatacagt aacaaaagta gacgggaaaa gctttcaaag
    16381 tacagaagca tttatggatt atgtaaaaag ccaaaaagtt ggacaaacaa tcgaaatcag
    16441 ttatttgcat aatggtgaag aaaaaacagc ttctggtgat ctgatcgagt taccaacaga
    16501 taaaaaggca ggaataggta tcacgcttac tgaccatacc gagatcgaat cagatacaga
    16561 tgttcgtatt aattctggct ctatcggtgg gccatcagcg ggattgatgt tcactttaga
    16621 aatctatgaa caagtcactg gtaaaaactt gcgacatggt aagcaaattg caggaactgg
    16681 aacgatcaac agtgaaggag aagttggccg aatcggagga atcgataaaa aagtggcaag
    16741 tgcggataaa gctggcgtag agattttctt tgcaccagat gatacgattt cagacgatgt
    16801 taagaaagaa tatcctgaaa taaaaaccaa ctacgaagaa gcaaaagctg ccgctaaaaa
    16861 attgaattcc tcaatgaaaa tcgtaccagt aaaaacagta caagaagcgc ttgactatct
    16921 agagaaaatg aaatgaaact aaataattaa agaggggttg tgacacgagt tctgtcacaa
    16981 cccctctttt tcgtcaaaaa tcttcacgat aaaaataaaa acaaaagaaa aaattgacgt
    17041 aattagatgt tgaagggacg attttgccag agctcactgt ttcttcaatt ttaaatgaaa
    17101 ggagtaatga atgattttta ctggtcattc tttgaaaaaa gaacaatgat agaaaaaatc
    17161 agcaagaaaa tactagcttc acccaaattg atcatttgga tgtctatgat agttcttatc
    17221 ttttgtatcg caagtggttt tttattatca aatttgctat tttatagaga aacagaagag
    17281 actgctgcaa aagaaatact ttcttcgttt agttccagtg gagaagatcc atcagctgaa
    17341 ggaaacgatg tagaagagga tgctcgtcca aaggtcatgt acaccgatat caaaggaagt
    17401 gtgaaggagc ctggtatcta ttccttttct tcagaagaac gggtctatga tgtactaaag
    17461 cgtgcaggtg gattattaga agaagcggat agcgatcgaa tcaacttttc tgcgaagata
    17521 gaagatcagc aagtcctata tatccctgct gtaggtgaag agctgccaga acatcttgat
    17581 cagttggcat cctcggaagg aaaacagcct acagcagatt ccgagcctcc caaaataaat
    17641 atcaatacag ccactccatc agaacttcag cagattcctg ggatcggatc agtcaaagcg
    17701 caagaaatca ttcgatttag agaagaaaac ggatccttcc agaaagtgga agacctgcag
    17761 gagatttctg gtatcggtga aaagacagtt gaaaaactaa aaaacttcgt tacaattaaa
    17821 tagaatgatt caaaatcgac tggcacgagc aataaatgga cgctaatgga tactaagaat
    17881 aatccaattc taagagcttg gcaggacgag gaggtagtat ttttggatag gaaatagctt
    17941 gattgttcgc aaagaaacat taaaaaaacc aatgctgaat gtctatctcc aaaataaaat
    18001 atctgggatt catattatga atacggctgt cagcggcaat aacagtcaag ctctacgaga
    18061 acgctttgcc aaagatgtac ttagctatac agcagataaa gtatttattc tcattgggac
    18121 gaatgatcta gcagagcata aacaactttc taaagaaacg taccaaaaaa tctgcagtgg
    18181 atgatagttc agctgaaaaa agactatagt tctgatcatg ctttctttat cacgccacca
    18241 gcagtcgatg agacaaaaca aaaatatcgg aataacaagt tgatcaaaga atatatctct
    18301 gttctcacag aagtagcaaa acgccatgat tgtgaagtat cgatttttat acggaatttc
    18361 ttgaacaaat ggattcgaag aatttttata atgggctaat ggatgacgga cttcatttcg
    18421 gcagtgaagg gtaggatctt ttgtcagaca ggatgattcg ttatatttaa agaatatttt
    18481 gtttaagcaa tgagtacttt ttaatcaact tgaaaaaacg gaggaatata aaacatgtta
    18541 gaaaaatctt caaaagcatg cgatacgaat aaagaaaaag ttactgataa gtcacataat
    18601 gaacgcattc cttgggacca gtactttatg gctcaggcag tactgttatc tttacgtagt
    18661 acgtgtacac gtttagaagt aggagcgaca ctcgttcgag aaaaaaggat cattgctggc
    18721 ggctataatg gagcagtcag tggagatgtc cactgcatag atgaagggtg ttacgtcgtg
    18781 gatggacact gcttgcgaac gatccatgca gagatgaatg cactcctaca gtgtgcaaaa
    18841 cttggtatcc cgacggaagg ttcagaaatc tatgtcacac attttccatg tttggcctgt
    18901 acaaaggctt tgttgcaagc tggtgtgaaa gggatcaatt atctccatga ctatcgaaat
    18961 gatccttatg cccaagcgtt gatcgaacaa ttaggtgtat ctgtacacca agttgcttta
    19021 gactcacagc atttttcaaa acttcaaaat gaattaacag gaaaacatgt ctagcgtttt
    19081 agaaaggcat gttcgaaatc ggttgatttt tccggcaatt acgtcgatga ttatctgttt
    19141 gttgtattac actgacaatt ggggttatcg tttgcttctt gccggaattt ttttatggtt
    19201 ttcgctaaaa gaaggacgtt ttatcgcgta cttgtgtatt ttgtgtagca taacggtatc
    19261 catttcttat aggctacaag agtattcatt tttagaggaa aaaacaacat ctgcgcaaat
    19321 tactgtattt acagatacga ttcgtgcaaa tggtaatttt ttgacaatgg aaggaaggtt
    19381 atcagagaat aaacaaaagc tccagctttc ttatcaagta aacacagaag cagaattgaa
    19441 aacatggctt aacttccgag gaaagggaat agaattgact gctgaaggcg agttcatctc
    19501 tccagcaaaa aagcggaatc tttataattt tgaccaagaa gcttacttca ggaaccatag
    19561 aatttctgga cggttccaaa ttcaaaagat tgaacagatc accatgcaaa atcactggcg
    19621 aaactggctt cagagaaaaa gaggatcttt tatctccttt gtccaatctg aatttccgca
    19681 aaaaacaagt gtatatatta attctttatt atggggctat aaagatgcag gattcagaga
    19741 accagaagaa atttttaggg agagtggttt gctgcatctg ttcagtctat cgggtctaca
    19801 catccaattt tatttagggt ggctgtatta tttatttcga cgtatcggat ggccactaca
    19861 cgtcagtatt attccgttga gtctactaac tatctgttat gtttctattg caggaagttc
    19921 tatttctgtt ttgcgtgcta gtttgctgtt tttattgcga ttgctagtca aattgctgaa
    19981 taagcattat tccaccatgg acttatttgc agttgtcttt tggcttcttt tactatttga
    20041 tcctcgattt ctttttcatg cgggtggaca gcttagtttt ttcatgagtt ttttgtttat
    20101 cctgatttct tttgatggag attggttgaa aaaaacggcc tctcacgtcc tgtttactat
    20161 gatcacgatg ccgttgattg tatggcattt ttatgagtgg cctattttag gtagtctttt
    20221 tacattactg attgcaccgg tattcaagtt tctttttctt cctggtgtgt tatttctcac
    20281 actcttcaat cgctggctgc cggaaacttt acttttgctg atagaagagg gattaatctt
    20341 atttgagcga ataatcgatt tttcttcagg gtcgtctctc attataggac gtttgcccct
    20401 tgtgctatct gggatgtata ttttcggatt actgatttgt atggataggt tgaaagaaca
    20461 acctgtgaaa cacttgtttt atgttggtct tttttctttt atttttttga ctttgccatt
    20521 tttacgcttt tcttcaacga tcgcttttat cgatgtcggt caaggagaca gtatcttttt
    20581 gcaaagtcct tttcgaaaag aaacgatttt gatcgatact ggaggtcagt tacactttga
    20641 aagagaaaag tgggcgcaag gtatgatacg tcctcctgct gaaagaaata tcgttccata
    20701 tctaaaaggt caggggataa gtgtaatcga taaactcatc ttgacacacg acgatacgga
    20761 tcatgtggga gaattaccca cgttatctca gcattttact atcaaaaaaa tttatatcgg
    20821 ttggggcgct ggacgaaatg agccattaca aaggcattta tctgaagctc aaaaaaatgg
    20881 tactgagatc attgaaatca aacatgggga caggatcagc ggttattatg atctttatgt
    20941 attgactcct tttgaaaagg gagagggaag aaatgaagat tcgattggtc tatggatgga
    21001 atataacaat aggcgctttt tatttttagg agatttgaat caagcaatgg aaaaacaatt
    21061 gcttttgatt tatcctaatc tgaaagccga tgtagtcaaa ttaggtcatc acggtagccg
    21121 tacgtcaagt aacacagatt ttctttccca tattgaagca aaacatggga ttatatcctg
    21181 cggcgtaaat aatcgttatg gtcacccgca cagcgaagta ttggagacac tagaagaaaa
    21241 tcagataaaa acatggcgaa cggatcaaca aggaatgatc acctatcgct ggcatccagt
    21301 ttttcatcct catgggctag tggaaacaat gatagactag taattctcat ctgctcatgg
    21361 taggataatt tttgaaagtg gtgagtaatt tgagtataca agcagagtta caaaaaatac
    21421 gtaaagaaaa acttgcatct tgttacttga ttttaggaac agaaaagttt ctccaagatc
    21481 aagttcggac agaaattgtc aaaaaaatcc aattagaggg cgaagatgac ttaaattttt
    21541 taagttttga catggaaaat gcaagtttag atgaggtagt agctgaagca gagacgttgc
    21601 cgtttttcgg agatcaacgg ttagtattcg tagagaatcc ttatttttta acaggtgaaa
    21661 aagtaaacaa tggaatcgaa caaaatactg atttattgac ggcttatttg aaagaaccat
    21721 tagaatcgac tgtattagta ttctttgcgc catatgaaaa gttggatgaa cggaaaaaag
    21781 taacgaaaca attgaaaaaa acagcaatta tcatcaatgt ccaacagttg aatgaaaaag
    21841 aagtacgtca atatctgatg aataccttag agaattcctc aatcaaaatg gaacgaagtg
    21901 caattgattt attcttgagg ttgaccgact tagatttatc caaaatgatg agagagttac
    21961 agaaattggt cctttatgga caaaatcaac agcaaatcac ggtaagagaa gtagaagaat
    22021 tagttcctaa aacgttagaa cataatattt ttgacatgac ccaatatatt ttaaaaggga
    22081 agacggaaca agctttgcgt ttatacgaag atttagttat gcaaggggaa gaaacaatca
    22141 aaattaatgc gattttgctt tctcaattac gtttattttt acagacaaaa tttcttgtaa
    22201 agattggtta tcagcaggca aatattgctg aaacattgaa aatccatcca tatcgtgtaa
    22261 aacttgccat gcaggaagta aaaaaattcg atgaacattt gctggtacag ttgtttgatc
    22321 aattagttga gatggattat caaatcaaaa cgggacaaat cgaaaaagaa ttaagttttc
    22381 aactattcgt tttgcgcaca gggcagttgt taagaaatcg ataagaagaa agacgagggc
    22441 aaagcatgcg ggaaggtcgt ttgtttttgt cctttttttg acaaataaaa aaatcggccg
    22501 aagccgattc ttttatttag cgattttttt gcttaaacga gatttgtcgc ggttagcttt
    22561 gtttttgtgg attagacctt ttgattcagc catatcaata gctttaacag cttctttata
    22621 taacgcatct acgttgtcag cacctgaagc tacagcatct tcaaattttt tgattgcagt
    22681 acgcatagcg cttgtttgag atgaattttt cacattagcg ttttcgcttg tacgaacacg
    22741 tttgattgca gattcaatgt ttggcattag tttcacctcc gataacttaa gttcttacgc
    22801 gtgttaccgc ataattcaac attcattatt atacctaaag gcagcgtgct ttgcaatatg
    22861 aacatgacaa gtttttttcc ttaacagtct tttattagga ttttttttca gtaaaaacgg
    22921 atagtacaag ctgaaaaaaa tcaaaaagac gctattgtgg gaggcaatag cgtcaaacca
    22981 aactagattt ttatttatct atttaaacaa agtttgaatg ggaatgtttg gtactaagtt
    23041 attatataac aaagagaacc gtttgcaaag ttttttttga aaagggatac gattccctgt
    23101 tgttctttgt ttttctatta agaaactgca gttattttat aaccagtatc ctttgatcag
    23161 taaaggctat tctggtgtac cgattatttt aatgtattca tcgatatttt ttatcaacat
    23221 tttttttatg accaactggt tccgacggtt ccatttgcca cttacttcca gcacatattt
    23281 atttaggggc aaacgtaaga tatattttgc caaatagcga tctttaagca ggccattgat
    23341 cacacggtcc tcaagctgca agttgatccc agtcagcatc attggctgtt cttgtaaaac
    23401 gtacgtatca attaaaagac cttcaaaagt ttccacaagc gtcttccttt cttttcaaag
    23461 taacctgaaa acaggcacta attcttccat tttgatattc gttgaagtgg atgcgatcat
    23521 tctttctttt gtccactgta atctagtata acaaaaaaga gcagaagagc caaaaagaaa
    23581 aaagagcatc gaattctata ggaagtctca ttggcacggc ctaaattatt acaaaacatt
    23641 gattaaaaaa agcccctgca tcgcaggagc atcccctata tcgttttaat aggtcaatca
    23701 aaaagcagta tcaaacacat tttgtatcaa atcaggaaat aatagataag ctaagcccag
    23761 aatcaaaagt accgctagaa gaaaccagcc gattgttcct atgaaatact ttaatttttc
    23821 gaacattgta tcacctcgtt gtcagtatat cacggatcag taaattgggg gtgttaagaa
    23881 tctcctaaaa tagacaaaat ttgttctatt ttcctaaagc aaatacttgt aagatacagt
    23941 aagtgatacc agaagcaatg accggtccgg cggcgattcc tcttaagaag acaacaccta
    24001 aaatcgtgcc gaaaacaagt gcaacagtga tttgtggatc agaagcaatc aatcctacac
    24061 ccttggaaga taagacagcc actagcccac cgcaaaaaac agctacccag ccaatagggg
    24121 atttaaaggc ttctaataga tcacgtagac cgatttctcc tgttgcgata gggattagaa
    24181 tagccgctgt gatgatcgtc accccaatat tgattccttt gccttgaagc caagggaaaa
    24241 aggaacggga ggctggtatg agcttcataa tcaaaacaaa agctgccgct atcatcagac
    24301 tttgattttt agctaaaatt gctacaagta aaataattcc taaaaaaagc caactttcca
    24361 tgaaatatat tcctcacttt cttcgaaaca atcataacat gtttttagaa agttacaaaa
    24421 gtaggaattc atccttcaag acctattcat aagctgtttt tttcataaaa taaacaaaat
    24481 cataaaaaaa cactttgctt atttgacaga agacagccgc gctttgtata gtggacaaga
    24541 cttacatttt tttgaagaat cagaaagatc aggtaaaggt cttagtaaac gcaagccatt
    24601 cagaataacc aatattgtgc ttccttcgtg tcctacaact ccaagaggta gatttaaaaa
    24661 ttgcaggaag ttagaagcaa tcaacaagag aataacaaga atcgaaaaaa taatattttg
    24721 tttgatgatc cgccgcattt ttaaagatag ccgatgactg tagacaaatt tatttaagtc
    24781 gcttttcatt aagacgatgt ctgcgatatc catagcggca tctgtccctt gccccatagc
    24841 gacacctaaa gaagcggctg ctaaagcagg cgcatcattg actccatcac caaccatcgc
    24901 atttacttgg taaagttcat tctctttttt gactagttgt gttttttctt ccggagtaca
    24961 gcttgcatag taatgttcaa tccctacttg ttcagcaatg gcctttgctg tgccagaatg
    25021 gtcgccggtt atcatacttg tatggatgtt ttgggattta aagtaggaaa ttacttgttg
    25081 ggtatttgct tttggaatat ctagcagacc taaaacagca attaactgct gatctttaga
    25141 aagataaatc acggttttgc cttgattttc caaacgttcg atcatttccg caatctcttt
    25201 gctgattatc atcgtctcag gatcatatgc gtgcttgcca actttccatt gcgcacctaa
    25261 ataggtagtt tgtaatccaa aaccagtaat ttcttctatt ggtaagtatt ggatttcttc
    25321 aggtattgtg caatcgaaat attgcaaaat cgcatgagca agtggatgcg tcgttttttg
    25381 ttccatagca acaagaagtt gttgtgccaa atccttttca tcaaagaaaa agctgtcagt
    25441 tacaactggt tttcctttag tgagcgttcc tgttttatca aaggcaatcg cttttaattc
    25501 agaaagcttt tctaaatgga taccgccttt gattaaaatc ccattttttg cagaatttga
    25561 aagagcagct agagaagctg gtgtagcaga tgcgacaagc gcacaaggag aagccacaac
    25621 taataatacc attcctcgat aaaagctttc atcccaactc caacctagga aaaagtgggg
    25681 gagaaggatc attagaggaa cagcaagcaa aatcaatttt acataattat tttcgattcg
    25741 ttcgataaat gaagctgttt gagttggcgt attctgcgct tcttctacta attggataat
    25801 cttagcaaag acagtttcgg aactcgtttt tgttactcga atcgtgatgg gatttccttg
    25861 attcagcgtt cctgcataca tctcatcgcc taaatatttt tctacaggca cagattcacc
    25921 gcttaatgca gactcatcga tcgttgcctg tgtgctttct aaaaagccat ctatcggaac
    25981 agctgctcct ttagcgacaa ataccaagtt atccaccttt agttgagaaa catctacttc
    26041 ttgcatttgt ccattttctt ttaataattg ggctttttgt ggttgaagat tcatcaaagc
    26101 agtaatttct ttttgacttt tattagttgt gtattcctct aatgcaccgc ttaaacaaaa
    26161 gatgaatgtc aacattgctc cttcaaagta attaccgatc aaacaggcac caatagcagc
    26221 taaagccatc aataaatcca cattcagtgt ccgatcattc cacaattctt tcaagccttc
    26281 cgatgtttgt ttatatccac cgatgataat ggcactgata taaaaaagtg gggtgatggt
    26341 aagagagaat cggtcgaaga taaaaccgaa gatcataaat aaaaggcagc cgattgttgc
    26401 tgcaaaagct tttttctgat tagtcatcgt tattagacct ccttgtcaaa acaaggatag
    26461 cataataaaa atgtaattga taataataac agctaaatga gaacgataat ggttatcatt
    26521 tggtgattaa gcttgatgtt gtttttatta tttataatga ttattattaa agaaaataaa
    26581 aaaccagaac atttctcaaa taagagaagt gttctggttt tttctacatt gcaataaacc
    26641 aataaccaat caaaatgata ccaaggataa tacgatacca gccaaaagcg gtaaagtcgt
    26701 ttttcttcaa atagcttagt aagaatttga tagctaagac ggaaaccaca aaagatacga
    26761 atgttcctac aagaagaaca acagtttctt ccattttgaa tgaattccct tcagctaaat
    26821 atttgacgat cttcaagccg cttgcgccaa acatgacagg aatccctaag aaaaatgaaa
    26881 actctgtagc gacaaaacgc gaagcaccaa tcaagataga tcctaggatc gttgcgccag
    26941 aacgggatgt tccgggaaca agtgccaaga cttggaataa cccaacaaga agagctgctt
    27001 ggtacgtaaa gtcattcaga gaagtccaac gaggtgtttt gtttttgttg cgtttttcga
    27061 caataataaa ggcaataccg taaacgatca aaacaatggc aactggtaag aacttgtgga
    27121 aatttgcttc taaccaatca tccaaaggca aaccaatgat gatggctggt atgcaagcaa
    27181 cgatcacttt aaaccaaagt gtccacgtat cttttttttc taccgttgat ttacttggag
    27241 aaaatgggtt taacttgtga aaataaagaa cgacgacagc caagatcgcc cctaattgga
    27301 taacgacatt gaacatcgac ataaattgtg aactggcatc cagtttgatg aattcatctg
    27361 ctaggatcaa atgaccagta ctactgatgg gcagccattc cgttattcct tcaataatac
    27421 cgagaatcac tgctttgata atatttgcaa aaaacataaa ttccttcctt cccaattaag
    27481 atacatctta tagtataccg ttattttttg agaaacccaa tctgtttttt aatattaaga
    27541 aaaacttgag aaataaagca gataagtaca cgaagagaaa aaagaagaca aagccctcgt
    27601 tcagactttg ccttttatga ttctctggta aaaattaaaa tatccccatt atagaaacga
    27661 gtcgcgacta tgtgttttcc cgttatcgat ataagacgta tcattgatgc cgtggataga
    27721 aaatcgaccg tttgcataag tgacctttgt tacgctgcca tttaaaagcc cagcacgaac
    27781 aggctgttta ggatcgatca gattaagcaa aaaggcgatc gtcaaaccat gagatacgac
    27841 taaaacattt ccaccaccat ttttttcgca gtgatgggcg atatcttcaa atcctgtcca
    27901 tacacggtct cgcaatgttt cataagtttc cgcccaccca gcggtatcta agttgtatac
    27961 agcattagca atttgttcaa aagaaacttc tgtggtaaac atttcatcat atgttttaaa
    28021 attcaataca cgagggatca caccccacat ttccatgtca tatccacctt caagagaccc
    28081 gaaacaccat tctctaatcc ttttttcaat aaaataagga atttcttttc ccttagaatg
    28141 ttctcctaga ataatacggg cagtttcgat tgcacgacca ctgtcactag aataagcaaa
    28201 tgaaaagtcg acatttttta acccgcgacc taaataatga atgccttctt gtccttgctt
    28261 tgtgaggggt gtatcacacc agccttgtac tcgttcaatt gtattgaaca tcgtttttcc
    28321 gtgtcgtacg atataaagtg tagtttctgt cattttattc tcctcctaag tcgttttaac
    28381 gattgaaaaa aggatttgtc cttttttcct gttcaatcgt cgttgcatcg ccatgtcctg
    28441 gatagactgc aaattcatca ggtaatacaa acagctgggt ccgaatacta tgaagtaatt
    28501 gttctaaatt ccctgtatac agatcggttc taccgatact tcctctaaac agggcgtcgc
    28561 cagaaacgac aaaatcatca aaaataaagc tgacgcttcc tatagaatgc cctggggtag
    28621 ggacgacgcg aaaagacatg tttcctaaag tataagtttt catttcaaat tcatattctg
    28681 caggcgagac aataacatca gcaatatcat catgtctacc gagtccagaa agattcaaaa
    28741 tcggatcacc tagccatttt tgctctagtg ggctgacata aactgggatt tgataatacg
    28801 cgcgtaaagg ttccactgca ccgatatgat cgtagtgagt atgggtaagc aaaatcgcta
    28861 ccggctgttg gtttgttttt ttgatttctt gtttgattcg ttccgcatct tctccagggt
    28921 caatgatcaa aagtgcttct tcgttgtaga ccaaataaca gttttcttca atcattcctg
    28981 tttttaatct ttcgatatgc atgtgcattc ctccttgata gccaaaactt ctttcatcca
    29041 ttagtataac gcattcattg ctaggaaaca aaaacaggta cttatttctc tttacttatc
    29101 tgaattatca gactatatga aaagtatttg gatattctaa agagaaggaa agtaagtttt
    29161 catagcattt taatatgtcc ttgttacatt agattcatcc aaaaatccaa attctatatc
    29221 ctatatccct gcctaatcag cagggtgttt ttttatgttt ggaaagagag ctctttttgc
    29281 taaactaggt tttttcaggt aatcttagag agaaagaggt ggtggaaatg atcaaacagt
    29341 caaaaatatc tgtgcggcac ttagtagaat ttattttgcg acgtggaagt atcgacaatc
    29401 gtaaaaaaag taaccatact gcattagaag gagcgagaat ccaccgcaag ctccaaaaag
    29461 atgcgggaga aaactatgaa aaagaagtct ttttaaaaac aacagttcaa ttagatgaat
    29521 attacctgat cgttgaaggc cgtgcagacg gtattttcaa aagagacggt atttattata
    29581 tagatgaaat caaaacctcc gaaccacgat ttgaagattt agaaccagaa caagtggaac
    29641 ttttctttca tcaagcaagg gtgtatgcct atatctatag tcaagaacat gatttacaag
    29701 aaataaatct gcaattgact tacttccaga cggcagaaga agtgatcacg agaaaggttg
    29761 aacatcagac aagagagcag ttagaaactt tttttaaaaa gctgactgaa gattataaag
    29821 aatggcttgt ttttcaagaa aactggcgca ctgtacgcaa tgcctctttg atggctttga
    29881 aattccccca tgatgagtat aggaaaggtc agcgtgaact agcggtagct gcttataaaa
    29941 cgatccgtac gaaacagaaa ttgtttgtag aagcacctac cggaacagga aaaacaatct
    30001 ctacgttatt ccccgctttg aaagctgtag gggaagaaga gggagaaaag atcttttatc
    30061 taacggctaa aacaataact agacaagtag ctgaagatgc tatgacagca ttaaaagata
    30121 caggagctga agtaaaaagt gtcactttga cggcgaaaga caagatctgc tttttagatg
    30181 agacgacctg taatccagat cagtgcccat atgccaatgg ttattacaat cggattaatg
    30241 aaggtttatg ggatcttttg aatcatgaga atcagatcac tcgtgaagtc atcgaaactt
    30301 atgcaagaaa gcatacactt tgtcctttcg aactatcatt agatgtcagt atctggtgtg
    30361 acgtaatcat tggggattac aattatttat ttgatccaac tgtttattta agacgttttt
    30421 ttgaagatga aaaaaacgag gactatctgt ttttgattga cgaagcacat aatttggtga
    30481 atcgttcccg agagatgtat tcggcagaac tatcttatga aaaaacgaaa cgaagtaagg
    30541 aagctatacc aaaggaattc aaaaaacttc atcgccgttt caacaaacta ttaaaagaat
    30601 ttgattcgat ccgagaaata gcaaaagaag atcattggga ttatcatcat caaaaagcac
    30661 cggctgaatc tttagtaaag gcaggttatc aattaagcga aaaaattaaa gaatggctgg
    30721 cagaatttcc tgagcaccca tcacaagaac aattgcttcc ttattatttt gatttgcttc
    30781 attttttgaa ggtcagtgaa ttttatgatg atcactacga aacaacagtc gaaaaaacgt
    30841 atcgcgatct gatagtaaaa gagttttgta ttgatccaag cttgtttctt gagcagtcgt
    30901 tggataaagg aaaaagcagt attctttttt ctgctagttt ttcaccactc tcttattatc
    30961 aggaaacact ggggggacaa aaaagtctgg cttacaaact gccgagtcct ttcccagaag
    31021 gaaaccagca agttttcatc gcaaattacc tagagacgac ttatcgaaaa agggaacaca
    31081 gtctggcaaa attagtagag acgattcatg cttttgctga tggaaaggtc gggaattatt
    31141 tcgttttctt tccttcttat caatatttag atcttgcggt ggaagcattc agacagcaat
    31201 atcccgacag caatgtgcta atccaagaaa cggacatgaa tgaggaagaa cgcgaacgat
    31261 ttttaacaaa atttcaaaca aatcccgctg aaacattgat tggattttgc gtacttggcg
    31321 ggattttttc agaaggaatc gatttgagag gggaccgatt gatcggcagt atgatcgtag
    31381 gtgtaggttt gccgcagatg aatcatgagc aggaactgat caaatcatat tatgatgaga
    31441 aggaacacct tggatttgct tacgcttatc agcttcctgg aatgaacaaa gtcttgcaag
    31501 ctggtggtcg agtgattcgt ggcatggcgg atcaaggaat cgttgtattg gcagatcaac
    31561 gattttctag tttttcttat cgacgtttat tcccacaaca ttggcaaaca gcaagagaag
    31621 tacgaagtgt tggggaattg gaagaaggaa tacagcgttt ttggttatca aaagaagcag
    31681 taaatcaaaa aaacgaggaa tgacagacgt cagcctcgtt ttttgattaa cgatagttag
    31741 tgaattgcaa ctcgattggt aagtcaagtt tacgtaataa agcaatcaca gcttgtagat
    31801 catcacggtt cttacctgtc actctaatct gatcttcttg gatttgtgct tttactttca
    31861 gtttttcatt tttgatagct gtagtgattt tcttcgcgtt ttcacgatcg attccgttga
    31921 ccaaatcggc agtttgtcga gctttgccac ctagagcgtg ttctgattcg gaaaaatgga
    31981 tatttttgat tggcacattt cttttgatca gcttaccaaa taatacatct ttgatctgtt
    32041 cgattttgaa atcatcatcg ctcaatacaa ctagattgct gttctctaat ttgatttcta
    32101 ctgtagaatc cttgaagtca aagcgattgg acaattcttt tgttgtttgc tgaatcgcat
    32161 tttttacttc ttctgtattg acttctgata caatgtcgaa gctagcatct tttgccatag
    32221 gaaccctcct gatatacata taaatattta ccatacatct atcttatcat aggattaggg
    32281 cagattctta cctgtttgct aaaattcttc tcatttgagt tcgaagtatt ttattttaat
    32341 agataaactg ttattcttac atttggaata ccaaagtagg ggagaggtgc ttgtgagtaa
    32401 aattatgact gcgtatcata aaaatgaaag attaaagaaa atagtggtta ttttgataac
    32461 cggattgacc gctgcggtcg ctttaaatta ttttttgatt ccagccaatg tcttttctgc
    32521 agggatgaat gggatcgctc aaatcatttc ctcgctttta tatgaatggt ttcatatcaa
    32581 gatagacaca ggaacattta tttttctttt gaatatccct gtgtttgtat tagggtttct
    32641 caaagtagga aaacgagcaa cgatccttag ttttgtgaat gttgtatgtg tatcggtgat
    32701 gacgatgctg cttccagtcg gacagatcac tgataatatt ttgatgaatg ccctagtagg
    32761 cggtgtttta ttaggtcttg gcgtaggatt atctttaaaa atgggcttta caactggtgg
    32821 gatggatatc atctctcttg tactttcaca aacaactgga aaaacagtcg gaaactacat
    32881 gctgattcta aatggtatca ttgtagtcat cgcaggcttc ttctttaact gggaaagtgc
    32941 cttgtatacg attatttcca tttatgcaat gactcaagta attgatgcca tccacacgag
    33001 tcatcaaaaa gtaacagcaa tgatcgttac cgtcaagcca gaagaggtaa cagaagaact
    33061 agcaaaacgg atggtacgtg gaatgacatt actgccgtct gtcggtggct attcaaaaaa
    33121 acaagggaac atgatcatga tggtcatcac ccgctacgaa atgtatgatc ttgatcagat
    33181 cgttcatgaa atcgacgacg atgcatttat caatatttta ccaactcatt cagtatttgg
    33241 ccggtttgca aatgaaaacg aacaaaaaat gtatcgaaca actggcgtat tgccagaaat
    33301 aaaaaatcgt tcacaaaaaa aatgaggaat caggtttatt ttagaataaa tttaatctat
    33361 tacatctaat aagaacattt ttataaattt aaacgagaat cttacttcta aattctcatt
    33421 tacaagcagc taaaaaaaga gtatgataaa taaaaatcgt gttaaggagt gtcgtacgat
    33481 gagcgaagca aaccaagaac ggtctggaca agaaatagaa ctaatcaaag aacgtattct
    33541 tgctgccttg gaaatggtga ttgatcctga attaggaatc gacatcgtca atttaggctt
    33601 gatttatgat atagaattca atcctgaaaa tggagaaaca gtcattaaga tgactctaac
    33661 aactatgggc tgtccattag cagatatttt aacagaatct atccatgacg cattaaaaga
    33721 agtgccagaa gtaacgaaag cagaagtcaa actagtctgg tatccagcat ggacgacaga
    33781 taagatgagc cgttatgcac ggattgcgtt ggggatacga tagaaatctt ggctgactag
    33841 ctatgagaag caaataggaa aaacaaaaaa gccttttatc ctttctagaa gaaaagagat
    33901 aaaaggtttc tttgtttata aaattttgtc tgctatatta taagttaatt aaataaatgt
    33961 gttctattga taaaggacat tcaaatccct ttccttttgg aatccttctt ttttacatca
    34021 gtttgatttt cgtatagtat tctttctcat cttcacgata aatcgcagcc ccattttttt
    34081 gttgtgcttt aagactgcca agattttttt tatttacaga caaaagtgcc tcatcaatgc
    34141 cgattttttt tgcctcttgt agtactaatg ctaatccttg tgtggcatat ccttttttac
    34201 gataattagg ggagatgccg taaccaatat ggccagcgcc gtttcttaat gtttcgttca
    34261 gtttatggcg caaattaaaa attcctacat actcatgttc atcactttct aaaataaaga
    34321 cggttgcggg aacgtaactc tcaggaagat cgattcctaa agacgttttt tcgcattttt
    34381 ttacataatc taaaaattca gcaaatgtat agccataagc attgttgata aatccactct
    34441 catttggtcc aaagctagtt tgtagctgat aagctttctt taaatctgct tcccatagtt
    34501 tgatcagttt catcgtattc acctctttaa gtaaccttat tcatttgttt tctactatag
    34561 cactattgtt gtaaatagcc atgcctattt ttatttattg tgtcatatcg tattaagaat
    34621 catcgaacaa tacaaatatg aattacaata aaaaaattat ttgaagttcg ttcattcgtt
    34681 acttatgatc tctagaagaa agaaaacgtg aattacttca gcgtctagaa ttccagaagg
    34741 atagtaatca acagaagaaa taatcaatat ccaaatgaaa gaagatagct gaatcgagaa
    34801 aaagaggaaa aaagaaatga agaaaaaaat aaaatagtta ctaatcccag agtcgttcgt
    34861 ttaacattga aatcgatcat atggataaaa cagatttttt caccaaccct caatagatga
    34921 tggagttgct cggatgaatt atttgtgtag atggcataag ggagggcgta cagaaatgaa
    34981 aaagaacaaa aatatgttct tgacaaaaaa cacatgtaat cctaaactaa aagatgtgat
    35041 gcggctatgg cggaattggc agacgcgcta gcttcaggcg ctagtgaggg tgacctcgtg
    35101 gaagttcgag tcttcttagc cgcataattt gtttttttat aaagagaagg tacacctgga
    35161 gcgatgagaa agaaaataac atattatcta aaagaatata ggaaaaattt taaacaacgg
    35221 aaccagattc aaataaatca aaaaaaattt gtgtgaaggt gggacagttt aaaacctaat
    35281 agattgattc aaatagtaga tttatagatt ctgatacaag aaatactggc aaacgatagt
    35341 taaggaataa agactaaact taaactcaaa aagaggtcat gcgaaagaat aaatcgcatg
    35401 acctcttact atattttgaa tgtgtttaaa cgttcaaata atgaatctta gtaccaaccg
    35461 tttgcttccc agaatgcttt cgccgcttcc caagaaccat aacgtgaagt aacatattgt
    35521 tctgctactc tttcttggtt tgcagcagag tagtcaccat ttaagtaaga agaatctaat
    35581 tggtaacggc cgatgtaacg tccgttcgtt gctgtgtaag aaccgcttga ttctttttgt
    35641 gcgatccatt cttttgcaga gcttgtactt gctggagtag cagcagtttc tgttacagca
    35701 ggagcaggtt gtgtttcagc aacaggcgct gcttgtgttt ccgtttgaac cgtttcttgt
    35761 acaacaggtt gttcagcttg aacaggttca gcttggactg gtgcttcttg aacggctgct
    35821 tgaggttcag cagctgcttg tgcagagcct tctgttggga ttgttaattg ttgtcctgag
    35881 ta
//
LOCUS       DACGLE010000021        35717 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0534
            SAMEA3304449-rid9560453.denovo.022, whole genome shotgun sequence.
ACCESSION   DACGLE010000021 DACGLE010000000
VERSION     DACGLE010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304449
            Sequence Read Archive: ERR985064
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 35717)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35717)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702225.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702225.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 20:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,045
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 27 of 67
            Pseudo Genes (incomplete)         :: 25 of 67
            Pseudo Genes (internal stop)      :: 19 of 67
            Pseudo Genes (multiple problems)  :: 4 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35717
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304449-rid9560453.denovo.022"
                     /strain="VREN0534"
                     /db_xref="taxon:1352"
     gene            complement(<1..705)
                     /locus_tag="IXT20_001170"
     CDS             complement(<1..705)
                     /locus_tag="IXT20_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HAQ4859627.1"
                     /translation="MLEVKKLNVCYNDFLALNEISLKIQEGALTGIIGPNGAGKSTLL
                     KAMLNIIPHQGEVSINNEDINKKLVKIAYVEQKADIDFTFPIKVKECVSMGTYAGMKV
                     FQRIKNAEWQRVSKALEKVDMGKYSNHQIGELSGGQFQRVLLARCLAQNADFIFLDEP
                     FVGIDLVSERIIMDTLKELKHQGKTVLIVHHDLSKVKKYFDNIILLNRNLIAHGSVES
                     VFNEENLKKAYGDTIFI"
     gene            1393..1551
                     /locus_tag="IXT20_001171"
     CDS             1393..1551
                     /locus_tag="IXT20_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ4859628.1"
                     /translation="MNKKQTCEFLGIDNNTLDKWIRIYNFPVIRIDGIVRFDIEDIKD
                     FMNTFKTI"
     gene            1623..2843
                     /locus_tag="IXT20_001172"
     CDS             1623..2843
                     /locus_tag="IXT20_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAQ4859629.1"
                     /translation="MPTKLSNGKYKTNLRYPKKFREITGITSEKYQKVFPTRQLAIKA
                     ENAMKRKIETVLREENANSLELKGKIKFKEFYESKWLPRYELGQTTRSKRAPSYVTIS
                     NTKDIFRLHILPMFGEYAMNYLNSNTEIISDELTKKSKEYANIKIIKGYVRSMFDIAE
                     ILNYIEFNRTTKIIQCITAPKKIALEERRIREGNQALSSEELIAWIEAVNTDLNNHLL
                     TLHDYTLFMLTLYLGDRKSETYALQWKYIDFEKQTVRLKHALDKYQKKKFTKGRKDTV
                     IQVPEIVMALLSKWKSVQAEQLLRLKINQTPDQYLFTYTKPSGEVNCPVHADYLNYRI
                     NSIKRRHPELAHLSPHKLRHTYATIARQGGANMNQISNALTHSDISTTKIYVNTPDVV
                     DKTVFEAFQKGLKN"
     gene            complement(2936..3328)
                     /gene="rpsI"
                     /locus_tag="IXT20_001173"
     CDS             complement(2936..3328)
                     /gene="rpsI"
                     /locus_tag="IXT20_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HAQ4859630.1"
                     /translation="MAQVQYIGTGRRKNAVARVRLVPGTGKITVNKKDVEEYIPHADL
                     REVINQPFAVTETKGAYDVFVNVNGGGYAGQSGAIRHGIARALLEVDPDFRLALKRAG
                     LLTRDARMVERKKPGLKKARKASQFSKR"
     gene            complement(3344..3787)
                     /gene="rplM"
                     /locus_tag="IXT20_001174"
     CDS             complement(3344..3787)
                     /gene="rplM"
                     /locus_tag="IXT20_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HAQ4859631.1"
                     /translation="MRTTYMAKPGEVERKWYVVDATDVPLGRLSTVVASVLRGKNKPT
                     FTPHLDTGDFVIVINADKVKLTGKKATDKIYYRHSNYPGGLKSITAGELRAKNSRRLI
                     ETSVKGMLPKNTLGRKQFTKLNVYGGAEHPHAAQQPEVLDITNLI"
     gene            complement(4306..4773)
                     /locus_tag="IXT20_001175"
     CDS             complement(4306..4773)
                     /locus_tag="IXT20_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HAQ4859632.1"
                     /translation="MKFQQTKEVLNQLVADLSQMSVVVHQTHWYMRGPGFLTLHPMMD
                     EFMDDLNAQLDEVSERLITLDGSPYSTLREFADNTKIPDEIGRWDRTIPERLETLVAG
                     YRYLADLYQKGIEVSGEEGDDPTQDIFIEFKTATEKRIWMIQAHLGKAPEIDA"
     gene            complement(4893..6431)
                     /locus_tag="IXT20_001176"
     CDS             complement(4893..6431)
                     /locus_tag="IXT20_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HAQ4859633.1"
                     /translation="MNVSIGLDIGTTQTKAVAFTDEAQEAASAYFRYPLIQETQGMAE
                     QDQELIFQGVCTVIKEVIKQLPSDTIIQFVSFSSAMHGLILLDEEKTPLSRMITWADN
                     RAADEAEKLKYTVLGKEFYRQTGTPIHPMTPLNKIKWLQNSQPELFKKTAYFVGIKDY
                     IFYRLFGELISDYGTASGTGYFDIHEFQWAEEVLDYLNIRIEQLPQVFPSTYQVTGLP
                     SKTAAELGLPNDISFVLGGADGPLSNLGLGAFGDTIAALTVGTSGALRFITTQPQFHP
                     EMETFCYVLDQKHWVVGGATSNGAGIFDWAGHTFLQEVVQSAKEKGENPYDALLSAIA
                     FVPAGANGLIFHPYLLGERAPLWDADAFGSFIGLQRQHTEKEMMKAILEGICLNLYRI
                     LKGVCQENQLTEIRATGGFAQSPIFRQMMADVLGYPLVFPTVTEASALGAVILGWRSI
                     GKIQSISEAQELIELKEQVGVQETDHKKYQKIYPLFVSTQKHLAQTYQQFAQLREELA
                     DSEK"
     gene            6511..7197
                     /locus_tag="IXT20_001177"
     CDS             6511..7197
                     /locus_tag="IXT20_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HAQ4859634.1"
                     /translation="MLLYFIIGCCIGSFLCLTAQRIPLGHSIIYPRSHCVKCCRSLSW
                     YELIPILSIIVQRFRCRYCRCRLPFYYLLAEALCGGLFAWFFTFSSARNFSTFIWMLS
                     ALLFSLMDLFYFMVHSHTLFCSWAILWIFWLQAGVFQWQSVLLTFIVGGVCLRYGQSY
                     LGAGDTLLMLSWSGGLSLEELLQILFLASLFGISVFSVYFICHKKKLEKLPFIPFLSS
                     ALLIVLHLRY"
     gene            complement(7398..7925)
                     /gene="msrA"
                     /locus_tag="IXT20_001178"
     CDS             complement(7398..7925)
                     /gene="msrA"
                     /locus_tag="IXT20_001178"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HAQ4859635.1"
                     /translation="MTKKAVFAGGCFWCMVKPFEEQPGIISVTSGYTGGHVPNPTYEQ
                     VTTGTTGHTEAVEIEYDPEVISYDQLVEIYWQQTDPTDALGQFADRGDSYRPVIYYND
                     EEERNIAERSKQALQDSGRFDKPIVTKIEPRSEFYPAEDYHQDYYKKNKLHYNLYREG
                     SGRAGFLRRHWGVKK"
     gene            complement(8009..8686)
                     /gene="pgmB"
                     /locus_tag="IXT20_001179"
     CDS             complement(8009..8686)
                     /gene="pgmB"
                     /locus_tag="IXT20_001179"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HAQ4859636.1"
                     /translation="MKIKAALFDLDGVLVDTARYHYEAWLVLANQLSIPFTEKENEQL
                     KGISRTESLERLLSFGKMEQKFSEKEKSAFAEQKNNLYLQAIQKMDETSVLPGAIAVL
                     EYLKKTNIKIGLGSASKNARLILEKTNLTSYFDVLIDGTQVSKAKPDPEVFLKGAQQL
                     NVPPNACLVIEDSEAGCQAALAGNMHVLGIGENINLPSAEYVIPDLTVFDQVRSFWHL
                     SEAVSRI"
     gene            complement(8683..9813)
                     /locus_tag="IXT20_001180"
     CDS             complement(8683..9813)
                     /locus_tag="IXT20_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HAQ4859637.1"
                     /translation="MRILIAPDSFKESLTALEAAQAIQSGFQEVFVQAEYDLLPIGDG
                     GEGTVSSLADSLRLKRQTAVVTNAFGKNKEIQYASDGQTAIFEMAEIVGLKDVPLNQQ
                     QPLRLSTRGVGELIRQLLQQGVRHIIIGVGGSSTNDGGIGMAEGLGYAFYDQDNQKVE
                     ALGENLGKIATVDKTNVPIELIKAKITVITDVTNLLCGKEGASFVFGPQKGLKENELA
                     VADQAMHDFYMRFAPQVIEASGSSAGGGMGAGLLAFASGKIVSGIDYVLDLLEFDKRV
                     QQADLVIVGEGRLDGQSLKGKAPVGIARRVPEGVPVIAICGSVGKGSEKAADVGISAV
                     FPILPAAVPLQEALQQATDNLHRTAKNIASLLQVVHFQGGNR"
     gene            complement(9813..11996)
                     /locus_tag="IXT20_001181"
     CDS             complement(9813..11996)
                     /locus_tag="IXT20_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HAQ4859638.1"
                     /translation="MKVEEKQFDLEYINKYASQMTIGNGFFGIRGSQEEDYRNQVRGM
                     FAAGVYNRPLGAESAELVNLPDVIRWEIKIDGEHFSLETMKVLAYERALDLEMGELKR
                     YALIETAQGKKVEVITCRVADQNNLHQAVLKVVIKPLTEDCQVDVKTGIDGQQTNFGT
                     QQLVEKELRVFGDDLISASYQTTESKIDIGIAAKFNKAGVFQAKNRRITASFSEKIRK
                     NTSFTLEKIIVLSTSLQQTDPFKKSIAQAQKSVSYTEILEQSMSYWTDFWHQHRITIQ
                     GDSSFDQLAMDFAAYHLQIMTPRNDPSVSVGAKGLTGEGYKGHVFWDTEIFILPFFLY
                     QFPEVAKNLLLYRYDRLEGARQKARDYHYQGALFPWESAHTGQEETPKFAALNIKTGT
                     RQEVASAAAEHHISADLAYAVQEYVQATEDQSFLETKGKTLIHETAAFWLSRAVKVDD
                     HLEIHRVIGPDEYTEYIDNNAYTNYLAHYNVALAVKYQAGDPLFLKECQNFLDKLYLP
                     VPNKEQLIPQDDTYLSKPVIDLTTYKERPGSQSILLDYARSEVNELQVSKQADLVMLL
                     YLLPEYFSQPIVSKNLHYYENKTIHDSSLSKAIHAIEACRIQEMEWAYHLFQEACLID
                     LGPDPHSSDDGIHAASLGALWLAVIFGFSGITVTNGTLSISPRLPSAWEKISFPFIWK
                     NHRLLFVLTKKQIVIKKETKETLPIFVNGDQYQLEKELILERRGK"
     gene            complement(11993..13018)
                     /locus_tag="IXT20_001182"
     CDS             complement(11993..13018)
                     /locus_tag="IXT20_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAQ4859639.1"
                     /translation="MKKVAIIGAGQVAEKVHAAYYQKRSDVLELAAVVDPVSQRAEDF
                     CRRTGFSHAYTSVTQMLNEISPDIVSVCTPNRFHFEHVLAALEANASVMCEKPPAMTA
                     DQARKMAETADKNGQILAYGFQHRFSDEATMIKNHLDVLGDIYYVEANALRRSGVPGW
                     GTFIDKELQGGGPLIDYGIHMLDTAMYITGFPKVKSVRAYSFQKIGTKKNSGKFGKWD
                     PSKFTVEDAIFGTLELENGKIIQLNTSFALNIREDKLNVAFCGDQAGANLYPGELFED
                     VDGKLVSLQKTTPVNDPHELSMAHFVNRALGDQTQMIADGWQGYVIQNLVENLYRSAE
                     TGEVITL"
     gene            complement(13063..13890)
                     /locus_tag="IXT20_001183"
     CDS             complement(13063..13890)
                     /locus_tag="IXT20_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HAQ4859640.1"
                     /translation="MKFGTQDQAFFPRDILEKFRFIKDCGFEEYEIDGKLLIENIDTV
                     KQAIKETGIPVVTACNGYDGWIGDFIEERRLNGVRQIAEILRALSEVGGKGIVIPAAW
                     GMFTYRLPPMVSPRSKEGDYKAVSESLRYLDKVAEETGTVIYLEPLNRYQDHMINLLA
                     DARHYIDSNQLKHVQIIADFYHMNIEEDDISKALTTQKEYVGHIHLADNHRYQPGSGA
                     IDFTRHFQTLLQNEYEGYMVYECRLRGENEVKIYQESLQYLKNCLETARHLNDLEKE"
     gene            complement(13887..14954)
                     /locus_tag="IXT20_001184"
     CDS             complement(13887..14954)
                     /locus_tag="IXT20_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HAQ4859641.1"
                     /translation="MKKLVALEPRIAGFKDYEDREIEKNEVRIRVIFATPKPGTEIVD
                     FRGLSPFIDEEFSEEWRLFLPREEGSQRGVVFGEFQLGNMIVGTIEETGSLVTEYQKG
                     ELVCTYGPIRETLITNGVDNYKLRKLDRLDQWKNAVCYDPAQFALGGIREAHVRAGDA
                     VAIFGLGAIGQIAIQLAKKAGATTIVGIDPIAQRRELAEKHGADYTLDPMKADVGFRL
                     KELTDKRGVDSVIETSGSAAALQASLIGIGYGGIISYVAFAKPFPAGFNLGREAHFNN
                     AKIVFARASSEPNPDYPRWNRKRIEDTCWELLMNGYLDCEEIIDPVVPFAESAEAYMR
                     YVDQHPEESIKMGVQVRGGEQ"
     gene            complement(14980..15816)
                     /locus_tag="IXT20_001185"
     CDS             complement(14980..15816)
                     /locus_tag="IXT20_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAQ4859642.1"
                     /translation="MKNKKSRKILFYATILLLLAVNLFPFLIMLMTSFKDSQEAIATT
                     QTIFPKEWTFEHYQDIFNAEIFPFIMYFKNSLVISLIASVTAVVIGISGAYALSKLRF
                     KARSKINASFYTVYMFSGILIVVPLFKIISGLGLYDTNTALIITMVVQTLPTAIFMLK
                     SYFDTIPTDIEEAAMIDGLSRIQIIIRIIIPLAISGIISVFVYCFMVSWNDYLFASIF
                     LSTSEKFTLPIGLNTLFSTPDYIWGRMMAASLVTALPVIIMYALSERFIKGDLVEGGV
                     KG"
     gene            complement(15813..16682)
                     /locus_tag="IXT20_001186"
     CDS             complement(15813..16682)
                     /locus_tag="IXT20_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ4859643.1"
                     /translation="MNRPRKHSDLKLAMGLLAPSMLLLAVLVLIPMLSNIQISFFDMP
                     LNPNSDAVFIGLSNYLEILSDSEFYRSLGLTVAYTFLVVAGSTALGLAVAILLNRKFR
                     FRKTVRSLILLSYVTPSISLIFAWKYMFNNSYGIVNDLLVDRLQLFNEAPLWFDQPVA
                     SFFMVVLFAIWRYYPYAFISFLAILQTVDKTLYEAAEMDGANAWQKFKIVTLPAIKPV
                     LATVIVLRSIWMFYMFTDVYLLTNKVNILGVYLYQTAFAFNDLGKAAAISILLFIIIA
                     AVVLLSRKKVDLK"
     gene            complement(16752..18035)
                     /locus_tag="IXT20_001187"
     CDS             complement(16752..18035)
                     /locus_tag="IXT20_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HAQ4859644.1"
                     /translation="MKKIIVTTCLLATGFLLTACGESQSSNESQQTTIQFMHSSVEQE
                     RLSVINELVADFEKENPDIKIKQVPVEESAFNTKIVTLARSGKLPEVMEVSQDFAKVM
                     DKDELIDQTAVQNVIEEAGEDNYYDGAKNLVRSEDGKSYIAAPISGWVQGIWYDKEKL
                     SEAGFSEPENWDDILKIAQHFTDKENKQYGIAMPTAEGTMSEQAFSQFALSNGANVLD
                     DEGEVTIDTEKMREALSFYQNLSQYTMPGSNDVTEIKDAFMNGTAPMAIYSTYILPSV
                     YEAGNSENIGFAIPTQKDQAVYGTVSGLTISSGLDEKQKEAAEAFTAFLSQPKNMEKW
                     VLMSPGGAQPVNKQVVELDGYKENEVIKSFGELPSEIASAFDEVQVFGLVGEKNFTKM
                     GDITSSGVIGKAVNQVTVGNEDVDQALADAQKNTK"
     gene            complement(18049..19734)
                     /locus_tag="IXT20_001188"
     CDS             complement(18049..19734)
                     /locus_tag="IXT20_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphorylase"
                     /protein_id="HAQ4859645.1"
                     /translation="MYTKLLQAIYRDSDLSEKTASMIEQKVSVYQQKKGQAGKLHWDE
                     EDIFLITYGDQFFEEQQPTLRTFKKFYQKYLADTFRVVHFLPFFPYTSDDGFSVVNYQ
                     AINDRLGTWEDIEEMRSETRLMFDFVCNHMSAESTWFQEYLNMNDTYKDFFIDLPLDT
                     DTSSVTRPRTSPLLTTFIDKNGKEKHVWTTFSEDQVDLNFKNPEVLLKMIDILLFYLE
                     KGASFIRLDAVGFLWKELGTSCIHLPETHKIIQLFRAIADEAAPGTVLITETNVPHKD
                     NISYFGNGENEAQMVYQFSLPPLTLQAIRSGNTSKLKEWLKTIDFASKKTTFFNFLAS
                     HDGIGLNPVRGILPEKTILELVEQLKKEGARVNYKKNPDGTTSPYEINATYLDALSSP
                     TDTTEWRSKRFLAAHSFLVTLPGVPAIYIQSVLGSRNDYKGVEKTGENRKINRQKFLL
                     ADIEKELQTDPLRTAVYGELTQMLSIRKKEKAFHPNSLIYLLDSADSFISFIREAKEE
                     KVLVIHNFSNQSAGYLLPKGDYLDLFSGQIIHCSQERKLIFQPYEYMWLKIIK"
     gene            20009..21034
                     /locus_tag="IXT20_001189"
     CDS             20009..21034
                     /locus_tag="IXT20_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAQ4859646.1"
                     /translation="MKPTIYDIAKKANVSKSTVSRVLNNQPNISDKAKASVLQAIKEL
                     NYEPSKIARGLSGNGFDAILALSHRTSHTTYGNPFFSDVIQSISAVSEQNNFDLILQT
                     AKTPEEELEKCAKKIQEKMIRGIIILSSTMDETLLFELDQYNIPIVVIGKVGNDYTNI
                     YSVDTDNFQDSYDLVETLIKNGHRHIGCLHAPTEVYVSNDRLNGYRQCLFDHHLDIRN
                     EWIIDGGFRLEDSMAAMNDLMKLRERPTAIFSTDALKSLSIYKVAEQSGKQIPDDLSI
                     AGFSDPLLSSLFSPQLTRIDIPTNQLGIKATELLFQLIHHQYPEEKRIILSTEITLTD
                     SIQQQEM"
     gene            complement(21629..25282)
                     /gene="rpoC"
                     /locus_tag="IXT20_001190"
     CDS             complement(21629..25282)
                     /gene="rpoC"
                     /locus_tag="IXT20_001190"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HAQ4859647.1"
                     /translation="MIDVNKFESMQIGLASPEKIRSWSYGEVKKPETINYRTLKPERE
                     GLFCERIFGPTKDWECACGKYKRIRYKGIVCDRCGVEVTRSKVRRERMGHIELAAPVS
                     HIWYFKGIPSRMGLVLDMSPRALEEIIYFASYVVIEPGNTNLEKKQLLTEREYREKRE
                     QYGQEFQAAMGAEAIKQLLDNVDLDAEAAELKEELKSAQGQKRTRAIRRLDILEAFRA
                     SGNQPSWMVMDVIPVIPPDLRPMVQLEGGRFATSDLNDLYRRVINRNNRLKRLLDLNA
                     PGIIVQNEKRMLQEAVDALIDNGRRGRPVTGPGNRPLKSLSHMLKGKQGRFRQNLLGK
                     RVDYSGRSVIVVGPFLKMYQCGLPKEMAIELFKPFVMRELVQRELASNIKNAKRKIER
                     QEDEVWDVLEDVIKEHPVLLNRAPTLHRLGIQAFEPVLVQGRAIRLHPLVCEAYNADF
                     DGDQMAVHVPLNEEAQAEARMLMLAAQNILNPKDGKPVVTPSQDMVLGNYYLTMEEEG
                     REGEGMVFRDQNEAVIAWRNGYVHLHSRIGVNPNSLGEKPFTEWQKERTMITTVGKII
                     FNEIMPPEFPYLNEPTDFNLTVQTPDKYFVEAGTDIPAHIKEQELVLPFKKKNLGNII
                     AEVFKRFKVTETSKMLDRMKDLGYKHSTHAGITVGIADISVLNEKQEIIENAHKQVET
                     ITKQFRRGLITDDERYERVIAVWNAAKDSIQQKLMEGLEAKNPIFMMSDSGARGNISN
                     FTQLAGMRGLMAAPNGRIMELPIISNFREGLSVLEMFISTHGARKGMTDTALKTADSG
                     YLTRRLVDVAQDVIIREEDCGTDRGLEIQAIREGNEIIESLEDRLVGRYTRKEVRHPE
                     TNELIIAGNQLISEDIAKQIVDAGVETVTIRSVFTCNTKHGVCKHCYGRNLATGSDVE
                     VGEAVGTIAAQSIGEPGTQLTMRTFHTGGVAGDDITQGLPRVQEIFEARNPKGQAVIT
                     EVTGDVIDISEDPATRTKEVTIKGKTDTRTYTVPYTARMKVAEGDMIHRGAPLTEGSI
                     DPKQLLQVRDVLSVENYLLREVQRVYRMQGVEIGDKHIEVMVRQMLRKVRVMDPGDTD
                     ILPGTLLDIADFKDQNYNTLVSGGVPATSRPVLLGITKASLETNSFLSAASFQETTRV
                     LTDAAIRGKKDPLLGLKENVIIGKIIPAGTGMQRYRDMEPKEVGVASENVYSISDIEA
                     QMAAEDAMNNQTK"
     gene            complement(25361..28978)
                     /gene="rpoB"
                     /locus_tag="IXT20_001191"
     CDS             complement(25361..28978)
                     /gene="rpoB"
                     /locus_tag="IXT20_001191"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HAQ4859648.1"
                     /translation="MAGHVVKYGKHRERRSFARISEVLELPNLIEIQTDSYQWFLDEG
                     LREMFEDILPIDDFNGNLSLEFVDYELKEPKYTVAEARAHDANYSAPLHVTLRLTNRE
                     TGEIKAQEVFFGDFPLMTEQGTFIINGAERVIVSQLVRSPGVYFHGKVDKNGKEGFGS
                     TVIPNRGAWLEMETDAKDISYVRIDRTRKIPLTVLVRALGFGSDDTIFEIFGDSETLR
                     NTVEKDLHKNASDSRTEEGLKDVYERLRPGEPKTADSSRNLLNARFFDPKRYDLANVG
                     RYKVNKKLDLKTRLLNLTLAETLVDPETGEIIVEKGTVLTHQVMETLAPFIDNGLNSV
                     TYYPSEDGVVTDPMTVQVIKVFSPRDPEREVNVIGNGYPEAAVKTVRPADIIASMSYF
                     LNLMEGIGNVDDIDHLGNRRIRSVGELLQNQFRIGLARMERVVRERMSIQDTETLTPQ
                     QLINIRPVVASIKEFFGSSQLSQFMDQTNPLGELTHKRRLSALGPGGLTRDRAGYEVR
                     DVHYSHYGRMCPIETPEGPNIGLINSLSSYAKVNKFGFIETPYRRVDRETGRVTDQID
                     YLTADIEDHYIVAQANSPLNEDGTFAQDVVMARAQSENLEVSIDKVDYMDVSPKQVVA
                     VATACIPFLENDDSNRALMGANMQRQAVPLINPQAPWVGTGMEYKSAHDSGAALLCKH
                     DGVVEYVDASEIRVRRDNGALDKYDVTKFRRSNSGTSYNQRPIVHLGEKVEKGDTLAD
                     GPSMEQGEMALGQNVLVGFMTWEGYNYEDAIIMSRRLVKDDVYTSIHIEEYESEARDT
                     KLGPEEITREIPNVGEDALKDLDEMGIIRIGAEVQDGDLLVGKVTPKGVTELSAEERL
                     LHAIFGEKAREVRDTSLRVPHGGGGIVHDVKIFTREAGDELSPGVNMLVRVYIVQKRK
                     IHEGDKMAGRHGNKGVVSRIMPEEDMPFLPDGTPIDIMLNPLGVPSRMNIGQVLELHL
                     GMAARQLGIHVATPVFDGASDEDVWETVREAGMASDAKTVLYDGRTGEPFDGRVSVGV
                     MYMIKLAHMVDDKLHARSIGPYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYGAAYTL
                     QEILTYKSDDVVGRVKTYEAIVKGEPIPKPGVPESFRVLVKELQSLGLDMRVLDIEET
                     EIELRDMDDEDDDLITVDALTKFAEQQTAKELEKKAAEQVEDEKDDVIQNFETAEDNL
                     D"
     gene            29706..30674
                     /locus_tag="IXT20_001192"
     CDS             29706..30674
                     /locus_tag="IXT20_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAQ4859649.1"
                     /translation="MKKNIIIGLGAALLLALGGCAQKDNSAEASDGQKTIGVLQLVQH
                     NSLDAAYKGFKEGLAEEGYKEGDNLTIDYQNAQNNQDNLKSMSEKLVKEDPDLLLGIA
                     TPAAQSLLNETTTIPMTVTAVTDLEQAHLVTSNEKPGGNVTGTTDMVPIEKQIGLLLN
                     IVPDAKTIGIMYNNGEANSKIQADLAEKELKKAGVKVKVLTANSTNDVQQVTKSLAKD
                     VDGIYIPTDNTFASAASVVGEVAKETKTPVVAGAVDQVKDGGLATVGIDYESLGKQTG
                     IMAAKILDGKNPSELPVETANDLELYVNKDMAKALGINPDSIKEAE"
     gene            30692..31597
                     /locus_tag="IXT20_001193"
     CDS             30692..31597
                     /locus_tag="IXT20_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ4859650.1"
                     /translation="MTNLLSLFLSSASQGVLWALLAIGVYVTFRILDIADLTAEGSFP
                     LGAGIAAVSITNGYHPLTACLLGFLGGAAAGLVSGLLHTKLKIPALLAGIITMTGLYS
                     VTSRVMGAPNISLLGQPTIFHWVESFGITKDNAVLIVGLLFALIVVCLLVLFLRTETG
                     LAIRATGDNLAMSEANGINTDNMKIIGYMISNGCIALSGSLLAQNNGFADLNSGIGTI
                     VIGLASIIIAEVLLRNQPLFLRLLTVVLGAVIYRFILALVFELNVEPSDSKLASAFVL
                     VICLSFPQIQQKFHLPQKKKGGSVK"
     gene            31594..32400
                     /locus_tag="IXT20_001194"
     CDS             31594..32400
                     /locus_tag="IXT20_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ4859651.1"
                     /translation="MNDVLTLENLHHTFEKGTINENHVLKGINLSLDPGDFVTIIGGN
                     GAGKSTLLNSIAGTFSVDQGQILLNGKNITKKSVVERSKSISRVFQDPKLGTAVRLTV
                     EENLALAMKRGKKRGFFRGVKPQDRTFFKDQLVRLNLGLENRLSSEIGLLSGGQRQAI
                     TLLMATLQRPELILLDEHTAALDPATSKTVMELTEKLITEQNLTAFMVTHDMEDAIRY
                     GSRLIMLHQGRIVVDIQQEEKQQLTVPQLLALFKQNSGTALKDDQVLLSS"
     gene            32557..33525
                     /locus_tag="IXT20_001195"
     CDS             32557..33525
                     /locus_tag="IXT20_001195"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HAQ4859652.1"
                     /translation="MTTKDKVLQLLKKTPDFLSGEKLAQQLEVSRTSIWKAIKELEKA
                     GYRFEHGPTGYRYLPSDVLDAKEIQQELRSFIPDLKVQTMLTSESTMKDAKLAAAQGD
                     SVSKLFVADTQEQARGRFGRPFFAEPGRGIYMSLLLHPDKRFDELPQYTIIAASACVK
                     AIESVTGRSASIKWVNDIYMDGKKVCGILSEAVSDMESGRISHIIIGIGLNFSVPQNH
                     FPADLQEKAISVFPDGQASVTRNQLISKIWQELFTYLSHPEKALDFYKEKSFVLGKTV
                     SFNQKGTTYVGLAKEIGDHGELIVQTNEQKMSLSSGEISLTSIRTQ"
     gene            complement(33842..34366)
                     /locus_tag="IXT20_001196"
     CDS             complement(33842..34366)
                     /locus_tag="IXT20_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HAQ4859653.1"
                     /translation="MDYLDMLCESVDRAYDRFERLFEGVSIEEANRFPVEDRAPQIKS
                     MTWLAWHTARELDFQIADLAGTSPIWHSQDWESRFPMTIADNEQDWKHSLEQAKQIQA
                     NQIEDLLGYLRGAADFAISYLRRLEPSELDEVIDENWTPPVTRGMRLVSIIDDAAMHS
                     GQVIYARRLLGLRD"
     gene            34653..>35717
                     /gene="scm"
                     /locus_tag="IXT20_001197"
     CDS             34653..>35717
                     /gene="scm"
                     /locus_tag="IXT20_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HAQ4859654.1"
                     /translation="MKKRASLLVLVALLFNIFSYGVAVSAETYIPKDPGNIYNDLTGN
                     YGLLGIASQFHVFAKNKTTVNAHTDGNIATHELDAKNNFGTELHEGLLNQEINYIQKT
                     DSFANSSGIPTGSTRMNKFVVGSNVDVALQDGHPTVNGSRLDHLTPDDFYKDRAGHEY
                     IDFEQEFAKLNVAANDLATITPAKTYTAADFPDMNNRTIDLTDLSDTGFLLVNIDAEV
                     LTMNTPLQIINPNDQVVVFNVINSSSALNVQSPIKYNDRSNHETEDFSDANISWNFGN
                     EMTDLTISAPFQGTILAPNATIRITQNQDGTIIGTNVILDAATNRWDPNEIFISDNGT
                     DTTDTSDSTDTSTTSDSSDSS"
BASE COUNT        10273 a         7540 c         6609 g        11295 t
ORIGIN      
        1 aataaatata gtatctccat aggctttttt taaattctct tcattaaata cagattctac
       61 acttccgtga gcaattagat ttcgattcaa aagaataata ttatcaaaat acttctttac
      121 cttgcttaaa tcatgatgca caattagcac tgtttttccc tgatgtttca attcttttaa
      181 cgtatccata attattcgtt cactaactaa gtctatccct acaaatggtt catctaaaaa
      241 tatgaaatcc gcattttgag caagacatct tgctaaaagt acacgttgaa attgtcctcc
      301 agataattct cctatttgat gattagaata ttttcccata tcgacctttt ccaaagcctt
      361 tgaaactctt tgccattctg catttttaat tctttgaaaa actttcattc cggcatatgt
      421 tcccatagat acacattctt ttacttttat tggaaaagta aaatcaatat cagctttttg
      481 ctctacatat gcaatcttca ccagtttttt attgatatct tcattattaa tggagacctc
      541 tccctgatga gggataatat ttaacattgc tttaagcaat gtagattttc cagcaccatt
      601 tggaccaata atacctgtta aagcaccttc ttgtattttt aaagaaatct cgttcaatgc
      661 taaaaaatcg ttataacaaa cattaagctt tttcacttct aacataactt tctccttcta
      721 aatgataatg attatcacga tcatatggta tacaaaaatt atattttagt caaccctaaa
      781 ttttataatt tagatttttt tctgttgctg aagattagaa tgacaaaact atttgaaact
      841 acgcgtatag taagtagctt tcaaaatata taaaaaagga gcgttctcaa atgagaacat
      901 gctcctaaaa ctactttatt ggcgtatgga aaaattatac ttaaagtgga tagcgggttg
      961 aataagttga taagcatcaa aacatttata aaaattattc aagactatgt ctgttccata
     1021 tacaaatatg aaaatcagta aactccattt aacttttttc gtagtgtgtc tttttataca
     1081 tgtttatcgt tattaatagt gaataggaaa aaacctattt ttgatcattg acaattaaat
     1141 acaatttttg aataacgatc cctgctattc gagctagaaa ctcaagtacg ccatgacctg
     1201 attagcgaca aatacttaga gatttctttt tgttctggga aaacaaaatg ccatgacaat
     1261 agcagcaaat aatgatactt ccgtaaggct cgccgtaaac tggggagagg tgaaattcct
     1321 atgtgtttac ccaaagcaat tcaaaaattc taatcttttt taggagatga ttttataaaa
     1381 ttgagagtct acttgaataa aaaacaaacc tgtgagtttc taggaattga taataatact
     1441 ttagataagt ggattcgtat atataatttt ccagtaatca gaattgacgg tatagttcgc
     1501 tttgacatag aggatatcaa agattttatg aatacattta aaacaatttg aaaatttctt
     1561 ctcgccgcat atactaagaa tagcgaaata tactgcctta ctttttaagg gagtgaggaa
     1621 ttttgcctac aaaactttca aatgggaaat ataaaactaa tctgaggtat ccaaaaaaat
     1681 tcagagaaat cactggaatt acatcagaaa agtatcaaaa agtatttcca actaggcaat
     1741 tggctattaa agccgaaaat gcaatgaaaa ggaaaattga aacagtttta agagaggaga
     1801 atgcaaattc actagaatta aaaggaaaaa taaaatttaa ggaattctat gaatcaaaat
     1861 ggcttcctag atatgaacta ggtcagacta ctagatcaaa aagagctcca agttacgtaa
     1921 caattagtaa tactaaagat atattccgat tgcacatcct tccaatgttt ggtgagtatg
     1981 caatgaacta tctaaattca aatactgaga taatatcaga tgaattaaca aaaaaatcga
     2041 aagaatacgc aaatataaaa attatcaaag gatatgtccg ttctatgttt gatattgcag
     2101 aaatactaaa ttatattgaa tttaaccgaa ctaccaaaat tattcaatgt atcactgcac
     2161 ctaaaaaaat tgctttagaa gaaagaagga tacgggaagg taatcaagca ttatcgtcag
     2221 aagagcttat tgcttggata gaagcagtaa acactgactt gaataatcac ttgcttactc
     2281 ttcatgacta tactcttttc atgcttaccc tatatcttgg tgatagaaaa agtgagacgt
     2341 atgctttgca atggaaatat atcgactttg agaaacaaac ggttcgcctt aaacatgctt
     2401 tggataaata tcaaaaaaag aaatttacaa aaggtcgaaa ggataccgta attcaagtac
     2461 ccgaaattgt aatggcatta ctaagcaaat ggaaatcagt gcaagctgag caattgttga
     2521 gattaaaaat taatcaaaca ccagatcagt atctctttac ctatactaaa ccatccggag
     2581 aagtaaattg ccctgttcac gctgattacc taaactatcg aatcaattca attaaaagac
     2641 gacatcctga attggctcat ttatctccac ataagcttcg tcatacctat gcaactatag
     2701 ctagacaagg cggagcaaat atgaatcaaa tatctaatgc tttaacacac tcagatattt
     2761 ctactacaaa gatatacgta aacacaccag atgtagttga taagactgtt ttcgaagcat
     2821 ttcaaaaagg attaaagaac taggatgaat gtgttctcga atgtgttctt aaactaaaaa
     2881 aagacacttt ccaaataatt gaaaagtgtc ttgaaacatt gatatacaaa gaatattaac
     2941 gttttgaaaa ctgtgaagct ttacgagctt ttttaagacc tggtttttta cgttcgacca
     3001 tacgtgcgtc acgagtaagt aagccagcac gtttaagagc taaacggaag tcaggatcaa
     3061 cttctagcaa tgcacgagcg ataccatgac ggatagctcc tgattgtcca gcgtatcctc
     3121 cgccgtttac gttaacaaat acgtcatatg cgccttttgt ttcagtaaca gcgaaaggtt
     3181 gattgattac ttcacgcaag tcagcatgtg gaatgtattc ttcaacatct tttttattta
     3241 cagtaatttt gccagttcct ggtactagac gtacgcgagc aactgcgttt ttacgacggc
     3301 ctgtgccgat atattgaact tgtgccaatg aaattccctc ctattagatt aagtttgtga
     3361 tgtctaaaac ttctggttgt tgtgctgcat gtggatgttc tgctccaccg tatacgttca
     3421 attttgtaaa ttgtttgcgg cctaaagtgt ttttaggaag catacctttt acagaagttt
     3481 cgatcaaacg acgagaattt ttagcacgca attcgccagc agtgattgat ttcaatcctc
     3541 ctgggtagtt gctgtgacgg taataaattt tgtcagtcgc ttttttacct gttaatttca
     3601 ctttgtctgc attgatgacg atgacgaaat cgccagtatc caaatgaggt gtgaaagttg
     3661 gtttattttt accacgtagt acagatgcaa caactgttga aagacgtccc aatggaacat
     3721 cagttgcgtc tactacatac catttacgtt ctacttcgcc tggtttggcc atatatgttg
     3781 tacgcacgtt attttcctcc aatttaagtt ctgatgtttg acatacactt cgagtttccg
     3841 gggctctttg tggggcaaac aataccattt aacatattac ccgctaatct agtcaatgtc
     3901 aacgtttttc tgcttgattg aaaaatttat atgaaacttt acttcttttc tttcttttat
     3961 ataaaagggt cgtttcctgt caaaatgaaa aacaaaaaga ggctgtgaca aaagtcttgt
     4021 cacaacccct tattttgaat aaacggtgtt ctcctgccaa ctcctcggtc acaagtcaca
     4081 atatccataa aacataagaa gctgtttttt aaaattgttt cttgtgactc ggagctgaac
     4141 ggcaggctta caacctcttg gtaaacggtg ttcaatcagc tgaccctcgg tattagctca
     4201 aaaaacggca aaatatgagg aactattttg acgtttgttt ctctaatact caggtcaaaa
     4261 cgctgatttc acaacctctt tttcggtcaa gttcaacaac agtttttacg cgtcgatctc
     4321 tggcgctttg cccaaatgag cttggatcat ccagatacgt ttttctgtgg ctgttttgaa
     4381 ttcgataaag atatcttgtg ttggatcgtc gccttcttct ccagaaactt cgattccttt
     4441 ttggtaaaga tctgctagat aacgatagcc tgcaacaagt gtttccaaac gttctggaat
     4501 agtacgatcc cagcggccaa tttcatcagg gatttttgtg ttatctgcaa attcacgcaa
     4561 tgtagaataa ggtgaaccgt ctaatgtgat caagcgttcg gatacttcat ctaactgagc
     4621 gttcaaatca tccatgaact catccatcat tgggtgcaat gtaaggaaac ctggtcctct
     4681 catgtaccaa tgtgtttggt gaacaacaac agacatttgg cttagatctg caactaattg
     4741 atttaatact tctttggttt gttggaattt catctttctt gcctcctaac attttcttac
     4801 acttaaaata atagtagact ttcaatgaaa agtacaacaa aatcatttac ctgagaacga
     4861 ttttcagttc gcttttttat ccgtaaagcc aatcattttt cagaatcagc taattcttct
     4921 cgcagttgag caaattgctg ataagtctgt gctaaatgct tttgtgtcga aacaaataat
     4981 ggatagattt tctgatattt tttatgatca gtttcttgta ctccaacttg ctctttcaat
     5041 tctatcaatt cctgtgcttc actgatactt tggatctttc cgatgcttct ccagcctaaa
     5101 atcacagctc ctaatgcgga tgcttctgtg acagtaggga aaacaagcgg gtaacccagt
     5161 acatcagcca tcatctgacg aaaaatcgga gattgagcaa acccgcctgt agcacggatc
     5221 tctgtgagct gattttcctg gcaaactcct tttaaaatcc gataaaggtt gagacagatc
     5281 ccttctagaa tcgccttcat catttctttt tctgtatgtt gccgctgtaa accgataaaa
     5341 ctgccaaacg catctgcgtc ccaaagcggt gctcgctcac caagtaaata gggatgaaaa
     5401 atcagaccat tggcacccgc tggcacaaaa gcgatagccg aaagcagtgc atcatatgga
     5461 ttttctcctt tttctttagc agattgaacg acttcctgta aaaatgtatg ccctgcccaa
     5521 tcaaagatgc cagcaccatt gctagtcgct cctcccacta cccaatgttt ctgatccaga
     5581 acataacaaa acgtctccat ttctggatgg aactgaggct gagtagtaat aaaacgaagt
     5641 gctccgctcg ttcctacggt caaagcggcg atcgtatcac caaaagcacc taaccctaga
     5701 ttagaaagcg gcccgtctgc ccctcccaaa acaaatgata tatcatttgg caaacctagt
     5761 tctgctgctg tttttgatgg tagacctgtt acttgatagg tggaaggaaa gacttgcggc
     5821 aattgctcta ttcgaatgtt caagtagtca agcacttctt ctgcccattg gaattcatgg
     5881 atatcaaaat atccagttcc tgaagctgtt ccgtagtcgc tgatcagttc gccaaacaat
     5941 ctataaaaga tatagtcttt gataccgaca aaataagcag tttttttgaa taattcaggt
     6001 tggctgtttt gcagccactt gattttgttc aatggtgtca ttggatgaat cggcgtccca
     6061 gtctgccgat aaaattcttt gccaagcacg gtatatttca gtttttctgc ttcgtccgct
     6121 gctcggttat ctgcccaagt gatcattcga gataacggtg ttttctcttc atccaacaat
     6181 atcaggccat gcatcgcaga ggagaaggag acaaattgaa tgattgtgtc tgaaggcaac
     6241 tgtttaatta cttcttttat aacggtgcag acaccttgaa agatcaattc ctgatcttgt
     6301 tctgccatcc cttgtgtttc ttggatcaaa gggtagcgaa aataagcgga tgctgcttct
     6361 tgggcttcat ctgtgaaagc aactgctttg gtttgtgtgg ttccgatatc taatccgatt
     6421 gaaacgttca ttttttcacc tcaaatttta tgtatgtaca aaaatttagt ttaagcttgc
     6481 gtatcttggt tttgaaaagg gggagatggc gtgttacttt attttatcat tggttgctgt
     6541 atcggatcat ttttatgttt aaccgcccag cggattccgt taggtcactc tattatttat
     6601 cctagatctc attgtgtcaa atgctgtcgt tcgctttctt ggtatgaatt gattccgatt
     6661 ctctcgatca tcgtccaacg atttcgctgc cgctattgca ggtgtcggtt gcccttctac
     6721 tatttgttgg cagaagcctt atgtggtggt ctttttgcct ggttctttac tttttcttcc
     6781 gcaagaaatt tttcgacgtt catatggatg ctttcggctt tgctattttc gttgatggat
     6841 ttattttact tcatggtcca ttctcacacg ctattctgtt cttgggcaat tttatggatt
     6901 ttttggttgc aggccggcgt cttccaatgg caaagtgtgc tgttaacgtt tatcgtaggc
     6961 ggtgtgtgcc ttcgctatgg tcaatcctat ctcggagctg gcgatactct gttgatgctt
     7021 tcttggagtg gtggcttgtc tctagaagag ttgcttcaga tcttgtttct tgctagtctg
     7081 tttggaatat ccgttttttc tgtttacttt atctgtcata aaaaaaagtt ggaaaagctg
     7141 cctttcattc cttttttgag cagtgcgttg ctgattgtcc ttcatcttag gtattgaaaa
     7201 aaggttgtga caaacgactt gtcacaacct ctgatatata gtgttctctt accaaatcct
     7261 tgtgacttgg agctaggcga caggttcaca acctctccac taaatggtgt tcaatcagct
     7321 gaccctcagt attagctcaa aatgctggtt ttacaacctc tgatatacgg tgttccataa
     7381 gtagcttttt cggcttctta tttcttgacg ccccaatgtc gacgaaggaa acctgcacgt
     7441 ccagaacctt ctcgataaag attgtaatgg agcttgtttt tcttgtaata atcctgatgg
     7501 taatcttctg caggataaaa ttcgcttctt ggttcgattt ttgtcacgat tggcttatcg
     7561 aatcgcccgc tgtcttgcaa tgcttgtttt gaacgctctg caatatttcg ttcttcttca
     7621 tcattatagt agatcactgg acgataagaa tcaccgcgat cagcgaactg tcctaatgcg
     7681 tcagtcggat cggtttgctg ccaatagatt tccaccaact gatcataaga aataacttct
     7741 ggatcatatt cgatctctac tgcttctgta tgccctgttg tgcctgttgt tacttgttca
     7801 taagttggat ttggtacatg cccgccagta taaccggatg tcacagaaat gatccctggc
     7861 tgctcttcaa atggtttgac catacaccaa aagcatccgc ctgcgaaaac tgcttttttt
     7921 gtcatataaa atcccccttt tttctcatca taccttgatt tccaaaagga ggataacgtt
     7981 ttgcttgtga cagaaaaaac ggaaactatt atatacgtga tacggcttct gataaatgcc
     8041 aaaacgaacg tacttgatca aaaacggtta aatcaggaat cacatattca gcagaaggaa
     8101 gatttatgtt ttcacctatt cccaatacat gcatgttacc agccaaagct gcttgacaac
     8161 cagcttctga atcttcaatg accaagcatg catttggcgg tacattcaat tgctgtgctc
     8221 ctttgagaaa aacttctgga tcaggttttg cttttgatac ttgcgtgccg tcaataagga
     8281 catcaaaata acttgtcaaa ttcgtttttt ctaaaatgag gcgtgcattt ttgcttgctg
     8341 atcccaatcc gatcttgatg tttgtttttt tcaagtactc aagaacagct attgcaccag
     8401 gcaaaacgga tgtttcatcc attttttgaa tcgcttgaag atatagattg tttttttgct
     8461 cagcaaaggc tgatttctct ttttctgaga atttctgttc catttttcca aatgacagca
     8521 atcgctctag tgactcggtt ctggatattc ctttcagttg ctcgttttct ttctcagtga
     8581 atggaatgga cagctggtta gctaaaacca gccacgcttc gtaatgataa cgagctgtgt
     8641 ctacaagaac gccatctaaa tcgaatagtg ctgctttgat tttcatcgat tacctccttg
     8701 aaaatgaacg acttgaagta aagatgcgat gtttttagct gtgcgatgaa gattatcagt
     8761 ggcttgctgt aaagcttctt gtaaaggcac agctgcagga aggatcggaa aaacggccga
     8821 aatgccaaca tctgctgctt tttcgcttcc tttaccaaca cttccgcaaa tagctatcac
     8881 aggaacgccc tctggtactc ttctagcaat cccaactggc gcttttcctt ttagactttg
     8941 tccatctaga cgtccttcgc ctacgattac gaggtctgct tgctgtaccc gtttatcgaa
     9001 ctccagtaaa tccaatacgt agtcgatgcc agagactatt ttccctgaag caaatgctaa
     9061 cagtcctgca cccattccac caccagcact actgcccgat gcctcgatta cttgaggggc
     9121 aaatctcata taaaaatcat gcatggcttg gtcagccact gctaattcat tttctttcaa
     9181 gcctttttgt ggtccgaaaa caaaagaagc gccttctttt ccgcataaaa gattagtaac
     9241 atcagtaatg acagtgattt tggctttaat cagctcaata ggcacgttcg ttttatcaac
     9301 tgtagcaatt ttacctagat tttctcccag tgcttctact ttttgattat cttgatcgta
     9361 aaaagcataa cctagacctt ctgccatgcc gatgccgcca tcgttggtac tacttccgcc
     9421 aacgccgatg atgatgtgtc gaacaccttg ttgcaacaat tgtcgtatca gttctcctac
     9481 tcctcgcgtg gataacctaa gtggctgttg ttggtttagt gggacatctt tcaatccgac
     9541 gatttctgcc atttcaaaaa tcgcagtttg accgtctgaa gcgtactgga tttctttgtt
     9601 tttgccaaat gcattagtaa cgactgctgt ttgccgcttc aaccttaaac tgtctgctaa
     9661 tgatgaaacc gttccttctc ctccatcgcc tataggtagc agatcatatt cagcctgaac
     9721 aaaaacttct tgaaaacctg attgaatggc ttgtgctgcc tctaaggctg tcagactttc
     9781 tttgaaagaa tctggcgcaa tcagaatacg cattattttc ccctccgttc caagatcagt
     9841 tctttttcca gctggtattg gtctccattg acaaaaatag gtaatgtttc tttcgtctct
     9901 ttttttatta ctatttgttt ttttgtcaaa acaaatagca acctgtgatt tttccaaata
     9961 aaaggaaaag aaattttttc ccatgctgaa ggaagtcttg gagaaataga aagagttcca
    10021 ttcgttactg taatacctga aaaaccaaat ataactgcta gccatagtgc tcctaatgaa
    10081 gcagcatgaa ttccgtcatc tgatgaatgc ggatctggtc ctaaatcaat caggcacgct
    10141 tcttggaata aatgatacgc ccattccatt tcttggatac ggcaagcctc tatggcgtga
    10201 atcgctttac tcaacgatga gtcgtggatt gttttgtttt catagtaatg caaattctta
    10261 gaaacgattg gttgagaaaa atactcaggt aataagtata acagcatgac aagatctgct
    10321 tgcttgctga cttggagttc atttacttcg ctacgggcat aatcaagtaa aatagactgg
    10381 ctgccaggac gttctttgta agtggtcaaa tcaataactg gcttactgag atacgtgtca
    10441 tcttgcggaa tcagctgttc tttgttaggc actggcaaat ataacttatc taaaaaattt
    10501 tgacattctt tcaaaaaaag cggatcgcca gcttgatatt taacagctaa ggccacatta
    10561 taatgcgcca gatagttcgt atatgcatta ttgtcgatat attctgtgta ttcatctgga
    10621 ccaatgactc tgtgtatctc taaatgatca tcgactttta cagctctgct cagccaaaaa
    10681 gctgctgttt catgaatgag cgtttttccc tttgtttcta aaaacgattg atcctctgtt
    10741 gcttggacat actcttgaac ggcataagca aggtctgcac tgatatgatg ttctgcggca
    10801 gctgaagcaa cttcttgtct tgttccggtt ttgatattca atgcagcaaa tttgggtgtc
    10861 tcttcctgac cagtgtgtgc gctttcccat gggaaaagtg caccttgata gtgataatct
    10921 cttgcttttt gccgtgcgcc ttctaatcga tcataacggt acagcagcaa attctttgct
    10981 acttcaggaa actgatataa aaagaaaggc aggataaaaa tttccgtatc ccagaaaaca
    11041 tgccctttat aaccttcgcc agttagtccc ttcgccccta cactaacact tggatcattg
    11101 cggggagtca taatctgtaa atggtaagca gcgaagtcca tcgctaactg atcgaaagaa
    11161 gaatcgcctt ggatcgttat ccgatgctga tgccaaaaat ctgtccaata agacatcgac
    11221 tgctctaaaa tttctgtata actaacgctt ttctgggctt gggcaatact tttcttaaac
    11281 gggtctgttt gctgaagaga ggtagagagc acgatgatct tctccagcgt aaaggacgta
    11341 ttttttctga tcttttctga aaaggaagcg gtgatccggc gattttttgc ttggaataca
    11401 cctgctttat tgaatttagc tgcaatccca atatcaattt tactttctgt tgtctggtag
    11461 gatgcactga tcaaatcgtc accaaatact cgcagttctt tttcaaccaa ctgctgtgta
    11521 ccaaaattgg tttgctgtcc atcgataccc gttttcacat ctacttggca gtcttcggtc
    11581 aacggcttga tgaccacttt caggactgcc tgatggagat tattttgatc agcaacacga
    11641 caagtaatga cttccacttt ttttccttga gcagtttcaa tgagcgcata gcgtttaagc
    11701 tctcccattt ctaaatctag tgcccgctcg tatgcaagaa ctttcatcgt ctcaagagaa
    11761 aaatgttcac cgtcgatttt tatttcccat ctgatgacat ctgggagatt cactaactcg
    11821 gctgattctg ctcctaaagg tcggttatat acaccagctg caaacattcc ccttacttga
    11881 ttccgatagt cttcctcctg gcttcctctg ataccaaaaa atccatttcc aatggtcatc
    11941 tgactcgcat acttattgat atactctaaa tcaaactgtt tttcttctac tttcataagg
    12001 taataacttc tcctgtttct gccgaacgat ataaattttc caccagattc tggatgacat
    12061 atccttgcca gccatctgca atcatctgag tctggtcacc gagcgcccga ttcacaaaat
    12121 gagccatact tagttcatgt gggtcattaa ctggcgtggt cttttgtaag gaaaccagct
    12181 ttccatctac atcttcgaat agttcgcctg gatacagatt agcacctgcc tgatcgccac
    12241 aaaaagccac attcagtttg tcttctctga tattcaaagc aaacgaagta ttcagttgga
    12301 tgatcttgcc gttttctaat tccagtgtgc caaaaattgc atcttctact gtgaatttgc
    12361 ttggatccca tttcccgaat ttccctgaat tttttttcgt accaattttt tggaagctgt
    12421 acgcccgaac gctttttact ttaggaaatc cagtaatata catcgctgta tcaagcatgt
    12481 gaatgccata atcgatcaaa ggcccgcctc cttgaagttc tttgtcgata aacgttcccc
    12541 agcctggaac accagaccga cgaagcgcat tcgcttctac gtaataaata tccccgagaa
    12601 catcaagatg attcttgatc attgttgctt cgtcagaaaa acgatgctgg aatccataag
    12661 ccaaaatctg cccgttttta tcggctgtct ctgccatctt tcttgcttga tcagctgtca
    12721 tagctggtgg tttctcgcac atcactgaag cattagcttc gagggcagcc aatacatgtt
    12781 cgaagtgaaa gcgattgggt gtacagacac tcacgatatc gggagaaatc tcattcagca
    12841 tttgtgtaac tgatgtatac gcatgagaaa atcctgtacg tcggcaaaag tcttctgctc
    12901 gctggctgac tggatcaacc acagcggcca attctaagac atctgatctt ttttgataat
    12961 aagcagcatg tactttttct gctacttgtc cagcgcctat gattgctact ttcttcattt
    13021 atttctctct cctttgctta tttacttatt gttattcttt ctctattctt tctctaaatc
    13081 atttaaatga cgcgctgttt ccagacagtt tttcaagtat tgcaatgatt cctgataaat
    13141 tttcacttca ttttctcctc gcaaacgaca ttcatatacc atataacctt catactcatt
    13201 ttgaagtagc gtttgaaaat ggcgagtaaa atcaattgcg ccagatcctg gttggtagcg
    13261 atgattatct gctaaatgga tatgacccac atactctttt tgcgtagtca atgcttttga
    13321 aatatcatct tcttcaatat tcatatgata aaaatcagcg atgatctgca catgtttcaa
    13381 ttgattagag tcaatgtaat gacgcgcatc cgctaacaaa ttgatcatat ggtcttggta
    13441 tcggttcaaa ggctccaaat aaataaccgt acctgtttct tcggctactt tatctaaata
    13501 acgaagtgat tcgctgactg ctttatagtc tccttctttg cttcggggtg aaaccatcgg
    13561 cggtaatcga taagtgaaca ttccccaagc tgctgggata acgattcctt tgcctccaac
    13621 ctcgcttagt gcacgcaaaa tctcagcaat ttgacgtaca ccattaagac gccgttcttc
    13681 gataaagtca ccgatccacc catcgtagcc gttacaagct gtcactactg ggatccctgt
    13741 ttctttgatt gcctgtttga ctgtgtcgat attttctatt aacaacttac cgtcgatttc
    13801 gtattcttca aatccacagt ctttgataaa tctaaatttt tctaaaatgt ctcttgggaa
    13861 aaatgcttga tcttgcgtac cgaatttcat tgttctcctc ctcggacttg aacgcccatt
    13921 ttgatacttt cttctgggtg ctggtctaca taacgcatgt aagcttctgc gctttctgca
    13981 aatggcacaa ctggatcaat gatttcttca caatccaagt aaccgttcat caataattcc
    14041 caacatgtgt cctcgattcg tttacggttc catcttgggt aatctgggtt tggttcacta
    14101 gatgctcgag caaagacgat ttttgcatta ttgaaatgtg cttcgcgacc aagattaaac
    14161 ccagctggaa aaggtttagc aaaggcaaca taagaaatga tcccgccata gccgatccct
    14221 attaaagaag cttgtaatgc ggcagcactg ccactcgttt caataactga atctactcca
    14281 cgtttatctg tcaattcctt tagacgaaaa ccgacatctg ctttcatagg atctagcgtg
    14341 taatcggcac cgtgtttttc tgctaattct ctacgctgcg ctatgggatc gatcccaacg
    14401 atggtagtag cacctgcttt tttagctaat tgaatcgcaa tttgcccgat agcgcctaaa
    14461 ccgaagattg ctacagcatc acctgctcga acatgtgctt cccggatacc acctaaagcg
    14521 aattgagcag gatcgtaaca tactgcattt ttccattgat ccaaccgatc aagtttacgt
    14581 agtttataat tatcaacacc gtttgtaatc agcgtttcac gaatgggtcc ataagtgcag
    14641 actaactctc ctttttgata ctctgtcacc aaactgcccg tttcttcgat cgtaccgaca
    14701 atcatatttc ctaattgaaa ttcgccaaat accacaccgc gttggcttcc ttcttctctt
    14761 ggcaagaata ggcgccattc ttctgaaaat tcttcatcga taaatggact taatcctcta
    14821 aagtcgacaa tttctgttcc tggttttgga gtagcgaaaa ttactcggat acgtacttcg
    14881 ttcttttcga tctcacggtc ttcgtaatct ttgaatccgg ctattcttgg ttctaatgca
    14941 actaattttt tcatcttttc tttcctcttt cttttctttt tatcctttta cgccgccttc
    15001 gactaaatca cctttaataa atcgttcaga taacgcatac ataatgatga ctggtaaagc
    15061 ggtcaccaat gaagcggcca tcattcgtcc ccaaatgtag tctggtgtac tgaataatgt
    15121 gttcaaaccg attggtaaag taaatttttc ggaggtcgac aggaaaatcg aagcaaataa
    15181 ataatcattc cacgaaacca taaagcaata aacgaagaca gaaatgattc cagaaatagc
    15241 caatggtata atgatccgaa taatgatctg gatccggcta agaccatcga tcattgctgc
    15301 ttcttcaata tcagtcggaa tcgtgtcaaa ataacttttt agcatgaata tcgcagtagg
    15361 aagtgtttga acgaccatcg tgataatcag tgctgtattg gtatcataca aacctagacc
    15421 ggaaatgatt ttaaataatg gaacaacgat caaaataccg gaaaacatat aaacggtgta
    15481 aaagctagca ttgattttcg agcgagcttt gaagcgtagt tttgacaaag cgtatgcccc
    15541 gctgatccca atcactactg cagttacgga ggctatcaag gagatcacta aactattttt
    15601 gaaatacatg atgaatggaa aaatctcagc attgaaaata tcctgataat gttcaaaagt
    15661 ccattctttc ggaaaaatag tttgtgtcgt tgcaattgct tcttgtgaat ccttgaatga
    15721 tgtcatcagc atgatcaaaa atggaaacaa attgacagct aatagcaaca aaatcgtcgc
    15781 gtaaaataag attttcctgc tctttttatt tttcatttca agtccacctt ctttcgtgag
    15841 agcaagacga ccgctgcaat aatgatgaac aatagaatcg atatagctgc tgcttttcca
    15901 agatcattga acgcaaaggc tgtctgatac aaataaacgc ctaagatatt gactttattc
    15961 gtcagtagat agacatctgt aaacatatag aacatccaga tagatcgtaa aacaatcaca
    16021 gttgctagta ctggtttgat tgccggcagt gtcacgatct tgaacttctg ccaagcattt
    16081 gcaccgtcca tttcagctgc ttcataaagc gttttgtcga ctgtttgcaa aatagccaaa
    16141 aatgaaataa acgcatacgg atagtatctc cagatagcaa acaaaacgac cataaagaag
    16201 ctagcaactg gctggtcgaa ccacagcggt gcttcgttga aaagctgcaa cctatctact
    16261 aacagatcat tgacgattcc ataactatta ttgaacatat atttccaagc aaaaatcaaa
    16321 gaaatagatg gcgtaacata acttaataaa atcagtgaac gcaccgtttt cctaaatctg
    16381 aacttgcggt taagcaaaat agctacggct aatcctaaag ctgtactgcc tgctacaact
    16441 aagaatgtgt aagcaactgt taaacctaaa gaacgataaa attcactatc acttaaaatt
    16501 tcaagataat tgcttaatcc gataaaaacg gcgtctgagt taggattcag cggcatatcg
    16561 aaaaaactga tctgaatatt ggaaagcatt ggaatcagca ctaaaacagc taataaaagc
    16621 atgcttggtg ctaaaagtcc catggctaat tttaagtcag aatgtttcct tggacgattc
    16681 attgaaacct ccccctgata atctgccaat agctatctac ttttttcgaa caccattggc
    16741 agaacttctt gttatttcgt atttttttgt gcgtcagcta aagcttgatc gacatcttcg
    16801 tttccgacag ttacttggtt cactgctttt ccgatcacac ctgagcttgt gatatctccc
    16861 attttcgtga aattcttttc cccgactagt ccaaagactt gtacttcatc aaaagcacta
    16921 gcaatctcag atggcagctc gccaaaagac ttgatgactt cattttcttt gtaaccatcc
    16981 agttcgacta cttgtttatt cactggttga gcaccaccag gagacatcaa gacccacttt
    17041 tccatgtttt tcggttgaga taagaaagct gtgaatgctt cagcagcctc tttttgtttc
    17101 tcgtctagac cagaagaaat cgtcaatcca gatactgtgc catagacagc ttgatctttt
    17161 tgtgttggga tagcaaaacc gatattttct gagttccccg cttcataaac agaaggcaaa
    17221 atgtaagttg aatagattgc cattggtgct gttccattca tgaaagcatc tttgatttct
    17281 gtcacatcat tagagcctgg catcgtgtat tgggataagt tttgataaaa cgacaatgct
    17341 tctctcatct tttccgtatc gatcgttacc tcgccttcat catccaaaac atttgctcca
    17401 tttgacaaag caaattgaga gaacgcttgc tcactcattg ttccttcagc agtaggcata
    17461 gcaattccat attgtttatt ctctttatct gtgaaatgtt gagcaatttt taaaatatca
    17521 tcccaatttt ctggttctga aaaaccagct tctgataatt tttctttgtc ataccaaata
    17581 ccttgaaccc agccgctaat tggtgcagca atatagcttt ttccatcttc tgaacgaaca
    17641 aggtttttgg ctccgtcata ataattatct tctccagctt cctcgataac gttttggact
    17701 gccgtctgat caatcagttc atctttatcc atcactttag caaaatcctg actgacttcc
    17761 attacttctg gtaatttacc ggaacgagct aacgtaacga tttttgtatt gaacgctgat
    17821 tcttctaccg gcacttgctt gattttgatg tctggatttt ctttttcaaa atcagcaacc
    17881 agttcattga tcacacttaa tcgttcctgt tcgacagaag agtgcatgaa ttgaatcgtt
    17941 gtctgttgtg attcgttaga gctttgactt tcaccacaag cagtaagaag aaatccagta
    18001 gctagcagac aggtagtaac aataattttt ttcatttttt tccacctctt attttattat
    18061 tttcagccac atatattcat acggctggaa tatcaacttt cgttcttgac tgcaatggat
    18121 aatttgtcca gaaaacaggt ctaggtaatc tccttttggt aaaagataac ctgctgattg
    18181 attggaaaaa ttatggataa ctaagacttt ctcttccttc gcttcgcgaa taaaactgat
    18241 aaaagaatct gctgagtcca gtaagtaaat caaactattc ggatggaagg ctttttcttt
    18301 ttttcgaata gatagcatct gggtcaattc accatatact gctgttctta atggatctgt
    18361 ctgcaactct ttctcaatat ctgctaacaa aaatttttgc cgattgattt ttcgattctc
    18421 tcctgttttt tctacgcctt tgtaatcatt tcgacttccc agcacacttt gaatataaat
    18481 tgccggcacg cctggtaacg ttaccaaaaa tgaatgagca gctaaaaagc gtttgcttct
    18541 ccattccgtc gtatctgttg gagaacttaa cgcatctaga tacgtagcgt ttatctcata
    18601 tggactggtc gttccatctg ggtttttctt gtagtttact cgggcaccct ctttcttcag
    18661 ctgttccact aattcaagga tcgttttttc cggcagaata cctcgtaccg gattcaatcc
    18721 gataccgtca tgggaggcta aaaaattaaa aaaagttgtt tttttcgatg cgaaatcgat
    18781 ggtttttaac cattctttca acttacttgt attacccgaa cgaatcgctt gaagggtcaa
    18841 aggtggtagc gaaaactgat agaccatctg tgcttcattc tcgccattcc caaaataact
    18901 gatattatct ttgtgtggca catttgtctc agtgatcaat accgttcctg gagctgcttc
    18961 atcagcgatt gcacggaata attggatgat tttatgcgtt tctggcaggt gtatacaaga
    19021 agttcctagt tctttccata aaaatcccac agcgtccaag cgaataaatg aagcgccttt
    19081 ttctaagtaa aacaaaagaa tatcgatcat tttcaataaa acttctggat ttttaaaatt
    19141 caagtctact tgatcttcgc taaaagtcgt ccatacgtgt ttttctttcc cgtttttgtc
    19201 aatgaacgtt gtaagtaacg ggctggtcct tggtctagtc acgctgcttg tatctgtatc
    19261 cagtggcaaa tcaataaaaa aatctttgta cgtatcattc atgtttaaat attcctgaaa
    19321 ccaagtactt tcggcagaca tatgattgca gacaaagtcg aacattaacc gtgtttcgct
    19381 tctcatttct tctatatctt cccatgtgcc caaacggtcg ttgattgcct gataatttac
    19441 aacggagaaa ccatcatcag atgtatatgg gaaaaatggc aagaaatgta caactctaaa
    19501 tgtatctgcc aaatactttt gataaaattt cttaaatgta cgcaaagttg gttgctgctc
    19561 ttcaaaaaat tgatccccat aagtaatcaa gaaaatatct tcttcgtccc aatgcagttt
    19621 gcctgcctgg cctttcttct gctgataaac actgactttt tgttcaatca tagaggcggt
    19681 tttctcagac aaatctgagt cgcgataaat cgcttgtaaa agttttgtat acatcggatt
    19741 cccctttctt ttttgggagc gttcccaacg atttgatcat attatggaaa acgcttactg
    19801 tcaatagcat ttttttaaat agttttgata tacacaaaaa tacaaataag aaagcttatg
    19861 tatcaataat taatagcttc ttaaaaaaat atggtctctc atgatcatgc aagctattcc
    19921 taaattttca aaatcataga caaaaaagaa tgtctattgt actatggaat tattctaaaa
    19981 cgctcccaaa aaggaggtgc atataaaatt gaaacctact atttatgaca ttgcgaaaaa
    20041 agctaatgtc tcaaaatcaa ctgtctccag agtattaaat aaccagccaa atatctctga
    20101 taaagcaaaa gcaagtgtac ttcaagcaat caaagaattg aattatgaac cgagcaaaat
    20161 tgcaagagga ctgtcaggaa atggttttga cgcgattcta gcattatctc atcgtacttc
    20221 tcatacgacc tatggcaatc cttttttttc agatgttatt caatccattt ctgccgtttc
    20281 tgaacaaaat aacttcgact tgattctaca aacggcaaaa acaccagaag aagagctaga
    20341 aaaatgcgcg aaaaagatcc aagaaaaaat gatccgagga attattattt taagctctac
    20401 gatggatgaa acacttcttt ttgaattgga tcaatacaat attcctattg tggtaatcgg
    20461 aaaagtcgga aacgactaca cgaacatcta ctcagtggac acggataatt tccaggatag
    20521 ttacgatttg gtggaaacac tgatcaagaa tggacaccgt catatcggct gtctccatgc
    20581 tccgacagaa gtttatgtat ccaatgatcg tctcaatggt taccgtcaat gcttatttga
    20641 tcaccaccta gatatccgta atgaatggat tatagacggc ggctttcgct tagaagatag
    20701 catggcagca atgaatgatt taatgaagct acgagaaaga ccaactgcga ttttttctac
    20761 cgatgcatta aaatcattga gtatttacaa agtggcagaa caatctggaa agcagatacc
    20821 tgatgatctt tcgatcgcag gattcagtga tcccttattg tcctccctat tttcgccaca
    20881 gttgactcgg atagacattc cgacaaatca gttaggaatc aaagccacag aacttttatt
    20941 ccaactaatc caccaccaat accctgaaga aaaaagaatc attctttcaa ccgagattac
    21001 tctcacagat tcgatccaac aacaagaaat gtagaacaga attataaaag aacagaaaca
    21061 ctgaggataa gtcagttact ctgttctttt ttcaatcact catccctcgt taccccctcc
    21121 cggtatttta gcgaattttt cttattttcc atctgtttta gatagctgtg gataactata
    21181 atgtttgtgt taaactattt tttccacagg gaagaaaaaa ctatttttaa ccacgttgtg
    21241 tataagtttt tatagttcgt ttttttaaac acatttaccc ttatttcaat ccatgttatt
    21301 tggggatact tatctctaaa acataaatag aataacagac atgtctactg gttttgtttt
    21361 attcgaggaa ttattcaata atagctagca aaatattaaa aataaccgct ctatcttccc
    21421 atcaatttat tttgcattca aaaaatgatc tatctttact tttgttgatt gacttctctt
    21481 ggaagatgat cgttccgata ccttaattgg atacgaaccg atgttgttaa gatcctttcc
    21541 cacttttctt ctagttagac aaattatttt agacaaacca aaaaagaggt tgtgacaata
    21601 atttgtcaca acctcttttt taaaatatct attttgtttg attgttcatt gcgtcttctg
    21661 cagccatttg cgcttcaata tctgaaatac tgtagacatt ttcgcttgct acaccgactt
    21721 cttttggttc catgtcacga taacgttgca taccagtacc agctgggatg atcttaccta
    21781 taataacatt ttctttcaaa ccaagaagag gatctttctt accgcggatc gcagcatccg
    21841 tcaagacacg agttgtttct tggaatgatg cagcagataa gaaactattt gtttctaatg
    21901 atgcttttgt gatacctagt aagacaggtc gagaagttgc aggaacgcct cctgagacta
    21961 gcgtattgta gttttgatct ttaaagtctg cgatgtctaa taatgtacct ggtaagatat
    22021 ctgtatcacc tggatccatt acacgaactt tacgcagcat ttgacgaacc attacttcga
    22081 tatgcttgtc gccgatttct accccttgca tacggtaaac acgttgtact tcacgaagca
    22141 agtagttttc aacagataat acatcgcgaa cttgcaacaa ttgttttgga tcgattgatc
    22201 cttctgtcaa tggtgcacca cggtggatca tatcgccttc tgctactttc atacgtgctg
    22261 tataaggaac agtatacgta cgtgtatctg tttttccttt gattgttact tctttggtac
    22321 gtgttgcagg atcttctgag atatcgatga catcccctgt tacttctgtg atcactgctt
    22381 gccctttcgg attacgtgct tcaaagattt cttggacacg aggaagacct tgagtgatat
    22441 cgtctccggc aaccccacct gtatggaatg tacgcatcgt caactgagta ccaggttcac
    22501 cgattgattg agcggcgatc gtaccaactg cttctccgac ttcaacgtca gagccagttg
    22561 caaggttgcg tccgtaacaa tgtttgcaga caccatgttt tgtattacat gtgaagacag
    22621 aacggattgt cactgtttcg acacctgcat caacgatttg ttttgcgatg tcttcggaaa
    22681 tcaattggtt tcctgcaatg atcaattcat ttgtttcagg atgacgtact tctttacggg
    22741 tgtaacgtcc cactaaacga tcttctaatg attcgatgat ttcatttccc tcgcggatgg
    22801 cttggatttc aagacctcgg tcagtaccgc aatcttcttc acggatgatt acatcttggg
    22861 ccacgtcaac cagacgacga gtcaagtaac ctgaatcggc agtcttcaag gctgtatcgg
    22921 tcatcccttt acgagccccg tgagttgaga taaacatttc caagacggat agtccttcac
    22981 ggaagttcga aatgattggc aattccatga ttcgaccatt aggagcggcc atcaaaccac
    23041 gcattccggc aagctgtgta aagttggaaa tgttaccacg agctccagaa tcagacatca
    23101 tgaagattgg atttttcgct tccaaacctt ccatcagttt ttgttggatg ctgtcttttg
    23161 cagcattcca aacagcaatg acacgttcat aacgttcgtc atctgtgatc agaccacgac
    23221 ggaattgttt tgtaatagtt tcaacttgtt tatgggcatt ttcaatgatt tcttgttttt
    23281 cattcagtac actgatgtca gcgatcccta ctgtgatacc ggcatgtgtc gaatgtttgt
    23341 accctaagtc tttcatacgg tcaagcatct tagaagtttc tgttactttg aatcgtttga
    23401 agacttctgc gatgatgttt cctaagtttt tcttcttgaa cggtaatact aattcttgct
    23461 ctttgatatg tgcaggaata tcagtaccag cttcaacaaa gtatttgtcc ggtgtttgga
    23521 cggtcaagtt gaagtctgtt ggttcattca gataagggaa ttcaggaggc atgatctcgt
    23581 tgaagatgat cttaccaact gttgtgatca tcgtacgttc tttttgccat tcagtaaatg
    23641 gtttttcacc taatgagtta gggttcacac cgatacgtga atgtaaatga acatatccgt
    23701 tgcgccatgc gataacggct tcgttttgat ctctgaagac cattccttcg ccttcgcgtc
    23761 cttcttcttc cattgtcaag tagtagttac ctagaaccat atcttgagaa ggagtaacaa
    23821 ctggtttacc atctttaggg ttcaagatgt tttgagcagc taacatcaac atacgtgctt
    23881 ctgcttgtgc ttcttcattc aatggaacgt ggacagccat ttggtctcca tcgaaatcgg
    23941 cattataagc ttcacataca agtggatgca aacggattgc gcgtccttgt actaaaactg
    24001 gttcgaatgc ttggatacca agtcggtgaa gcgtaggtgc ccggttcagc aatactgggt
    24061 gttccttgat tacgtcttct aaaacatccc atacttcgtc ttcttgacgt tcgattttac
    24121 gtttcgcatt tttgatgttt gaagctagtt cgcgttgaac aagctcacgc atcacgaatg
    24181 gtttgaataa ttcaatggcc atttcttttg gaagtccaca ttggtacatc ttcaagaaag
    24241 gtccaacaac gataacggaa cgacctgagt agtcgacacg tttacctagc aagttttgac
    24301 ggaaacgacc ttgtttccct ttcaacatat gagaaagaga tttcaatggg cggttacctg
    24361 gtccagtaac tggacgtccg cgacgaccgt tatcgatcaa tgcatctact gcttcttgca
    24421 gcatacgttt ttcattttga acgatgattc caggtgcatt caagtctaac aaacgtttca
    24481 aacggttatt acggttgatt acacgacggt aaagatcatt caagtcactt gttgcaaaac
    24541 gtccaccttc taattgaacc attgggcgta aatctggtgg aataacaggg ataacgtcca
    24601 taaccatcca gcttggttga tttcctgaag cgcggaatgc ttctaaaata tccaaacggc
    24661 ggattgcacg tgtacgtttt tgtccttgtg cagatttcaa ttcttctttc aattcggctg
    24721 cttctgcatc tagatcaaca ttatcaagta attgtttaat cgcttctgca cccatagcag
    24781 cttggaattc ttgtccatat tgttcacgtt tttcgcgata ttcgcgttct gtcaacaatt
    24841 gttttttctc taaattcgta tttcctggtt cgatgactac atatgaagca aaataaatga
    24901 tttcttccaa agcgcgtggg ctcatgtcta atacaagacc catacgtgaa ggaatccctt
    24961 taaagtacca gatatgtgaa acaggagctg ctaattcaat atgtcccatg cgttcacggc
    25021 gaactttcga acgagtaact tccactccgc atcgatcaca aacgattcct ttataacgga
    25081 tacgtttgta ttttccgcag gcacattccc agtcttttgt aggaccaaaa atccgctcac
    25141 agaacaaccc ttcgcgttca ggttttaagg tacgatagtt aatcgtctca ggttttttta
    25201 cttcaccgta agaccagctt ctgatttttt cgggagaagc caaacctatt tgcatacttt
    25261 cgaatttatt tacatcgatc aaaaggggtt ccctccattt catttaatgg caaaccgcaa
    25321 gacggctgat cttcgaccgc cttgcagctc atagaccgga ttagtctaaa ttatcttctg
    25381 cggtttcaaa gttttggatg acatcgtctt tttcatcttc cacttgttca gcagctttct
    25441 tttctaattc tttggcagtt tgctgttcgg caaattttgt caaagcatca acagtgatca
    25501 aatcgtcgtc ttcatcgtcc atgtcgcgaa gttcgatctc tgtttcttcg atatccaaga
    25561 cacgcatgtc cagacctagt gattgcaatt ctttcaccaa tacgcggaat gattccggta
    25621 cacctggttt tggaattggt tcacccttga cgattgcttc gtatgttttc acacgtccga
    25681 ctacgtcatc tgatttgtat gtcaagattt cttgtaaagt ataagccgca ccgtaagctt
    25741 ccagtgccca tacttccatt tcaccaaaac gttgtccacc aaattgagct ttacctccca
    25801 atggttgttg tgtaaccaat gagtaaggtc cgattgaacg tgcatgcaat ttgtcatcga
    25861 ccatgtgagc aagtttgatc atgtacatca caccgacaga cacacgtcca tcaaatggtt
    25921 cacctgtacg tccgtcgtac agaacagttt tcgcatcgct ggccataccg gcttcacgaa
    25981 ctgtttccca tacgtcttca tcacttgcac catcaaatac tggcgttgct acgtggatac
    26041 ctaactgacg tgcagccata cccaagtgta attccaatac ttgcccgatg ttcatacgtg
    26101 aaggcacccc taaaggattc agcatgatat cgattggtgt tccatcaggt aggaatggca
    26161 tatcttcttc cggcataata cgggatacaa cccctttatt tccgtgacga ccggccattt
    26221 tatccccttc atggatttta cgtttttgaa cgatataaac acgaactaac atgtttacgc
    26281 ctggtgacaa ttcatctcca gcttcacgag taaagatttt cacatcgtga acgataccgc
    26341 cgccgccatg tggtacacgt aatgatgtat ctcggacttc acgagctttt tcgccaaaga
    26401 tcgcatgcaa tagacgttct tctgcagata attctgttac cccttttggc gtgactttcc
    26461 ctacaagcaa atcgccatct tggacttctg caccaatacg gatgataccc atttcgtcta
    26521 aatcttttaa tgcgtcttcc ccgacattag ggatttcgcg tgtgatttct tcaggtccta
    26581 attttgtatc gcgggcttct gattcatatt cttcaatatg gatagaagtg tatacgtcat
    26641 cttttaccaa acgacggctc atgatgatcg catcttcata gttgtaccct tcccaagtca
    26701 tgaaaccaac taaaacgttt tggcctagag ccatttcgcc ttgttccata gatggtccgt
    26761 ctgccagagt atcccctttt tcgactttct cacccaaatg aacgattgga cgttggttgt
    26821 agcttgttcc ggagtttgaa cgacggaatt ttgttacatc gtatttatct aatgcaccgt
    26881 tgtcgcggcg tacgcggatt tcagaagcat caacgtattc aacgacaccg tcatgtttac
    26941 ataacaaagc agctcctgag tcatgcgctg atttgtattc cataccagta ccaacccatg
    27001 gtgcttgagg attgatcaac ggcactgctt gacgctgcat gttggcaccc atcaaggcac
    27061 ggttggagtc atcgttttcc aagaaaggaa tacatgctgt cgcaacggca actacttgtt
    27121 taggtgatac gtccatgtag tctactttat caatagatac ttctaagttt tcactttgag
    27181 cacgagccat aacaacgtct tgagcaaatg ttccatcttc attcaatggg ctgtttgctt
    27241 gggctacgat gtaatggtct tcgatatcag ctgtcaagta gtcgatttgg tcagtaacac
    27301 gacctgtttc acggtctaca cgacgataag gtgtttcgat aaatccgaat ttatttactt
    27361 tcgcataact ggacaaacta ttgatcaacc cgatgtttgg gccttcagga gtttcgatcg
    27421 gacacatacg gccatagtgg gagtagtgaa cgtcacgaac ttcataaccg gcgcggtcac
    27481 gagtcaatcc gccaggtcct aaggctgaaa gacgacgttt gtgtgtcaat tcgccaagag
    27541 ggtttgtttg atccatgaac tgtgacaact gagaagaacc aaagaattct ttgatacttg
    27601 ccacaactgg acggatattg atcaattgtt gaggtgtcaa tgtttcagta tcttggatcg
    27661 acattctttc acgaaccaca cgttccatac gtgctagccc gatacggaat tggttttgta
    27721 aaagttcacc gactgaacgg atacgacgat ttcctaagtg gtcgatatca tcgacattgc
    27781 cgataccttc catcaagttc aagaaatagc tcattgaagc aatgatgtcg gctggacgaa
    27841 ccgtttttac agctgcttct ggatagccgt ttccgatcac gtttacttca cgttcagggt
    27901 ctcttggtga aaatactttg atgacttgga cagtcattgg atctgtgacc acaccgtctt
    27961 ctgatggata gtaagtcact gaatttaggc cattatcaat gaaaggagct agtgtttcca
    28021 tgacttggtg agtcagaact gttccttttt caacgatgat ctcgcctgtt tctggatcaa
    28081 ctaatgtttc agctaatgtc agattcaata gacgtgtttt caaatctaat tttttgttta
    28141 ctttgtaacg accaacgttt gctaagtcgt aacgttttgg atcgaagaaa cgagcattca
    28201 gcaagttacg tgagctgtct gctgtttttg gttcgcctgg acgcaaacgt tcgtaaacgt
    28261 ctttcaaacc ttcttctgtt cttgagtcgc ttgcgttttt atgcaagtct ttttcgactg
    28321 tattgcgtaa tgtttcgctg tcgccgaaga tttcaaagat cgtatcatcg gaaccaaatc
    28381 ctaacgcacg aactaatacg gttaaaggaa tcttacgtgt acggtcgata cgtacataag
    28441 agatgtcttt tgcatcagtt tccatttcta gccaagctcc acggttaggg ataacagtag
    28501 aaccgaatcc ttcttttccg tttttgtcga cttttccatg gaaatagaca cctggtgaac
    28561 ggactaactg agaaacgatt actcgctctg ccccgttgat gatgaatgtt ccttgttctg
    28621 tcattaatgg gaaatcaccg aagaatactt cttgtgcttt gatttcgcca gtctcgcggt
    28681 ttgtcaaacg caatgttaca tgtaatggtg cagagtagtt tgcatcatgc gcacgagctt
    28741 ctgctactgt atactttggt tctttcaatt cataatctac aaattccaaa gatagattgc
    28801 cattgaagtc atcgattggc aaaatgtctt caaacatttc tctaagtccc tcatctaaga
    28861 accattggta agagtctgtt tgaatttcga tcaaatttgg taattccaac acttcactga
    28921 ttcttgcgaa acttctacgt tcgcgatgtt ttccgtattt tactacgtgt ccagccaagc
    28981 tcttcacccc tctaaaatgt cattcaaaaa gactacccga tcaattgtta caataatatt
    29041 tcaaatatta tttttaggta acaacagaca ttttttgcgg cttttaggca aaaaaaaacc
    29101 aaaaatagaa cattctcttg aaaccttact tcgtgagctt ctacttttgt ttccttatat
    29161 taaagccgaa acggacaata tttcgtttct gccttttctt catcagatag tttttgcaac
    29221 ttcatatact acacttattt tctataaaag tcaacacttc ctggcgtttc ttctgctttt
    29281 tcgcaacatt ttcttaatgt tttcaatttt atgttttcaa ttatctgaca attcttaatt
    29341 gacagacctt tttatctcgg ttatacttta gaaaattcat tttgtggagg aacttctatg
    29401 cgaaccaaca tcaaccaaat cgcaccaaca caactgaata acttttactt tagctacttt
    29461 agataggttt gtattcatca tgatggatac aaacgttaaa cgacagtttg tatctatgat
    29521 ggctaaagaa ctttgtattg cagcaaaaag ccacgtagac aaaatgctaa gtggctgagc
    29581 agtgaaaata caaagtaaaa gtatcaagca ttctactttt tcaatttatt ttccgacttt
    29641 tcaatccatt tggacatttt gtctgaatgg attttttgtt tcccaaaatt aaggaggaaa
    29701 aagaaatgaa aaaaaatata atcatcggat taggcgctgc cctgctacta gctttaggag
    29761 gttgcgcaca aaaagacaat tccgcagagg catcagatgg acaaaaaaca attggcgtat
    29821 tgcaacttgt acaacataac tcgttagatg ctgcctataa aggattcaaa gaaggattag
    29881 cagaagaagg gtataaagaa ggcgataatc taacaatcga ttatcaaaat gcccaaaaca
    29941 atcaagacaa cctgaaaagt atgagtgaga aactcgtcaa agaagatcct gatttgctac
    30001 ttggaatcgc cactccagca gcacaatctt tattaaatga aacaaccact atcccgatga
    30061 ctgtcacagc ggtcacagac ttggaacaag cccatttagt cacatccaat gaaaaacctg
    30121 gcggcaatgt gactggcacg acagatatgg tgccaatcga aaaacaaatc ggcttactgt
    30181 taaatatcgt gccagacgcc aaaacaatcg gcatcatgta taacaacggt gaagcaaact
    30241 ctaagatcca agctgatttg gcagaaaaag aattgaaaaa agcgggcgtc aaagtcaaag
    30301 tactgacagc aaattccaca aatgatgtcc aacaagtgac gaaaagtcta gctaaagatg
    30361 tagatggaat ctatattcct actgataaca cgttcgcaag tgcggcaagt gttgtcggtg
    30421 aagtcgcaaa agaaacaaaa acacctgtcg ttgctggcgc agttgatcaa gtcaaagacg
    30481 gcggtctagc aacagtagga atcgactacg aaagcttggg caaacaaaca gggatcatgg
    30541 cagcaaaaat cttagacggc aagaatccaa gtgaacttcc tgtcgaaaca gcgaatgatt
    30601 tggaactgta tgtcaataaa gacatggcca aagcattagg catcaatcca gacagcatca
    30661 aagaagctga ataaaacaca ggaggattct catgacaaat ttgctctctc ttttcttatc
    30721 atctgcatcg caaggggtct tatgggccct acttgcgatt ggtgtatacg tcactttccg
    30781 tatcttggac attgccgact tgactgctga aggaagcttt cctttaggtg cagggatcgc
    30841 ggcagtaagt attacaaatg gctatcatcc attgacagct tgtcttctag gctttttagg
    30901 gggtgcggca gcagggcttg tttccggact gcttcacaca aaattgaaaa ttcccgccct
    30961 tcttgcaggg atcatcacca tgactggttt atattctgtc acatcccgtg tgatgggtgc
    31021 gccgaacatc tctttattgg gtcagccaac gatctttcat tgggtcgagt cattcggcat
    31081 aacaaaagac aacgctgtac tgatcgtcgg tctgcttttt gctttgatcg ttgtatgttt
    31141 acttgttttg ttcttacgta cagaaacagg acttgctatt cgtgcaactg gcgataatct
    31201 ggcaatgagc gaagcaaacg ggatcaacac agataatatg aagatcatcg gttatatgat
    31261 ttctaatgga tgcattgctc tttctggctc cttacttgcg caaaacaacg gctttgctga
    31321 tttaaattct ggtattggaa cgatcgtaat tggacttgct tctattatca tcgcagaagt
    31381 tttgttacgt aaccagccac tatttctccg tctcttgact gtcgttttag gtgcggtcat
    31441 ttatcgtttt attttagcgt tagtcttcga actgaatgtt gaaccaagtg attcgaaact
    31501 tgcttctgct ttcgtgctag tgatctgtct ttctttcccg cagatccagc aaaaattcca
    31561 tttacctcaa aaaaagaaag gcggtagtgt caaatgaatg acgtcttaac attggaaaat
    31621 ctccatcata cctttgaaaa aggaacgatc aacgaaaatc atgtcctgaa aggcattaat
    31681 ctttccttag atcccggcga cttcgtgacg atcatcggtg gaaatggggc tggtaaatct
    31741 actttgctga acagcatcgc cggtacattt tctgtcgatc agggtcaaat tttattgaat
    31801 ggaaaaaata tcacgaaaaa atctgttgtc gaacgttcga aatccatcag tcgcgtattc
    31861 caagatccta aactagggac agccgttcgt ttaacggtag aagaaaatct tgctcttgct
    31921 atgaaacgag gaaaaaaacg cggcttcttc cgtggtgtca aaccacaaga ccgaacattt
    31981 ttcaaagacc agctggttcg gctaaatttg gggttggaaa atcgtttatc ctcagaaatc
    32041 ggtttgctgt ctggaggaca acgtcaagct attacgttat tgatggccac attacagcgt
    32101 ccagaactga tcttattaga tgaacacact gcagcgcttg accctgctac ttccaaaacg
    32161 gtgatggaat tgacagaaaa gctgatcact gagcagaatc tcactgcttt tatggtcact
    32221 catgacatgg aagacgcgat ccgctatggg agtcgactga tcatgctgca tcaaggaagg
    32281 atcgtcgtag atatccagca agaggaaaaa cagcagctca cagtgcccca attattagcg
    32341 ttgttcaaac aaaacagcgg cacggcatta aaagacgatc aagtactttt atcttcataa
    32401 tcagaaagag gatgggacaa aaaatgaacg cccaccctct tcttttttct ctttccttct
    32461 ttcgtttttg ctttatgtat caaccgttgt ttcttcgaaa actattttgt tctctccttc
    32521 ttcctgatag aatggatggg aaaggagctt gatgccatga caacgaaaga caaggtactt
    32581 caattattga aaaagacacc agattttctt tccggagaaa agttggcaca gcaactggaa
    32641 gtttcccgga caagtatttg gaaagccatc aaagaattgg aaaaggcagg ctatcgtttt
    32701 gagcacgggc ctacagggta tcgttacctg ccctctgacg tactagatgc aaaagaaatc
    32761 cagcaagagc tacggtcgtt tattcctgat ttgaaagtac aaacgatgtt gacttctgaa
    32821 tctacgatga aagatgccaa actggcagca gctcaaggag attctgtttc gaagttattt
    32881 gttgctgata cacaagaaca agctcgtgga cgttttggac ggcctttttt cgctgaacct
    32941 ggacgaggaa tctacatgag tctcttgctc catccagaca aacggtttga tgaattgcct
    33001 caatatacga tcatcgcagc cagtgcctgt gtgaaagcca ttgaatctgt aaccggtcgt
    33061 tctgcttcga tcaaatgggt caatgatatc tacatggatg gaaaaaaagt ctgtgggatt
    33121 ttatctgaag cagtcagtga tatggaaagc ggcaggatca gtcatatcat cattgggatc
    33181 gggcttaatt tttctgttcc tcaaaatcat tttccagctg atctccaaga aaaagccatc
    33241 tctgtctttc ccgacggaca agctagtgtc acaaggaatc agctaatcag caaaatctgg
    33301 caagaattgt tcacttatct ttctcaccca gaaaaagcgt tggacttcta taaagaaaaa
    33361 tcattcgtat tgggaaaaac cgtgtcgttc aatcaaaaag gaacgactta tgtcggtctt
    33421 gcaaaagaaa tcggtgatca tggcgaactg atcgtccaaa caaatgaaca gaagatgagt
    33481 ttgtcttctg gcgaaatcag tctcacatct atccgtacgc aataattatc agatttcaca
    33541 aaaggacacc tgatctcata cagtgtcctt ttttctgaat tctcttcaaa agactctcct
    33601 tattctatca gctgacagtt tctctatcct ctagaaaaag gtattctaaa aatatctata
    33661 aagcatggta cactttcttt aaatgaatca atgcaaaaaa caagagagtc atcagtttac
    33721 acgatcaaaa gagatagcag gccgcttcct ttttcaatct tatagataga ccatgggtaa
    33781 cgataaaaac aaaaaagaac ttgcgactta ttttgccgta agttcttttt tagctagtgc
    33841 cttaatctct taatcccaac aaacgtctcg catagatcac ctgaccagag tgcatagcag
    33901 catcatcaat aatagaaacg agacgcattc ctcgtgtgac tggtggtgtc cagttctcgt
    33961 caattacttc atctagctcg gaaggttcca gccttcttaa gtaagaaata gcaaaatcag
    34021 cagctcctcg caagtatcct agcagatctt ctatttgatt agcttggatc tgctttgctt
    34081 gttccagact atgcttccaa tcttgttcgt tatctgctat cgtcatcgga aaccggcttt
    34141 cccagtcttg actatgccaa attggagaag ttcctgctaa atcagcaatc tggaaatcta
    34201 attctcgagc ggtatgccaa gccagccaag tcattgattt gatctgcggg gctctatctt
    34261 ccactggaaa acgattggcc tcctcaatag agacgccttc aaacaaacgt tcaaaccgat
    34321 cgtatgcccg atccacactc tcgcatagca tatctaaata atccatttat tttcctcctt
    34381 tttatttatc ctacttactt tttcctaatt ctaggatacc tacttatatg ttactacaca
    34441 cccttatttt ttacacttta acagtcacca tcatcaatag tttggagatt gaaagaaaaa
    34501 gatagtgtcg aactatcaat tgaacaagaa gcaacaatat gaacgtttca ttaatattct
    34561 ttaaataaac aatgattttt caaacgaaca gtttaagact aggagaaaaa gagtataata
    34621 ggaaaatgtt tgaaaggaaa ggaaggggaa aaatgaaaaa aagggcaagt ttactagtcc
    34681 tagttgcttt gttgttcaat atcttttcgt atggtgtggc cgtttcagcg gaaacataca
    34741 ttcctaaaga ccctgggaat atttataatg atctaactgg taactatggc ttgttaggaa
    34801 ttgctagtca attccacgta tttgctaaaa ataagacaac tgtgaatgca catacagacg
    34861 gaaatatagc aacacatgaa cttgatgcaa aaaataattt tggtacagag cttcatgaag
    34921 gtttattgaa ccaagaaata aattatatac aaaaaactga ttctttcgct aattcttcag
    34981 gcatacctac aggtagcaca agaatgaata aatttgtcgt tggcagtaac gtggatgtag
    35041 cacttcaaga tggtcatcct acagtcaatg gatccaggct tgatcactta actccagatg
    35101 atttttataa agatcgtgct gggcatgaat atattgattt cgagcaggaa tttgctaaat
    35161 tgaatgtagc agcaaatgat cttgcaacga tcactccagc taaaacatat actgctgcag
    35221 attttccaga catgaacaat cggacaatcg atttaactga tttatcagat accgggtttt
    35281 tattggtaaa tatcgatgca gaagtgttaa cgatgaatac gccacttcaa atcattaatc
    35341 caaatgatca agtagttgta tttaatgtca tcaacagttc ttctgcattg aacgtacagt
    35401 caccaatcaa atacaatgat cgttcaaatc atgaaacaga agatttttcg gatgcgaata
    35461 tctcttggaa ttttggaaat gagatgactg atttgacaat cagtgctcct tttcaaggaa
    35521 caatccttgc gcctaatgct actatccgca ttactcaaaa ccaagacgga acgattatag
    35581 gaacaaatgt gattttagat gcagctacca atcgttggga tcctaacgaa atatttatca
    35641 gcgacaatgg tacagatacg acagacacaa gcgacagtac agatacatct accacaagtg
    35701 atagctcaga ttcatca
//
LOCUS       DACGLE010000022        34661 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0534
            SAMEA3304449-rid9560453.denovo.023, whole genome shotgun sequence.
ACCESSION   DACGLE010000022 DACGLE010000000
VERSION     DACGLE010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304449
            Sequence Read Archive: ERR985064
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34661)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34661)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702225.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702225.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 20:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,045
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 27 of 67
            Pseudo Genes (incomplete)         :: 25 of 67
            Pseudo Genes (internal stop)      :: 19 of 67
            Pseudo Genes (multiple problems)  :: 4 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34661
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304449-rid9560453.denovo.023"
                     /strain="VREN0534"
                     /db_xref="taxon:1352"
     gene            complement(313..2205)
                     /locus_tag="IXT20_001198"
     CDS             complement(313..2205)
                     /locus_tag="IXT20_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ4859655.1"
                     /translation="MSKTNTRPSHGPAGGPGHGGPGRNLGVKGEKPKNFWGTVRRLFG
                     YMSKRMVAIIAVFVLAIAAVIFQIQTPKVLGKATTEIFKGVMAGAQQMQQGQQVTKFP
                     IDFDKIAQIIATVIVMYLISAVFNFLQQFIMTRVSQRTVYELRHDLEAKMNRVPISYY
                     DTHTNGDIMSRAINDMDNIASTLQQNLTQFVTSAVTLIGVIVMMLSISWQLTLVALLM
                     IPLSLIIVMIVAPKSQVFFADQQKSLGLLNNQVEETYGGHTIVKTFNHTEKDQEVFEK
                     ENQNLYAAGWKAQFISAIIMPLMNFVKNLGYVFVAVLGGVKVANGNMTLGDVQAFLQY
                     TTQFSQPITQLASLMNTIQSTVASAERVFEVLDEEEMSNEKSGLPEEKDTPYKVRFEN
                     VQFGYTPDNLLMTDFNLDVKPGEMVAIVGPTGAGKTTLINLLERFYDVSGGSIRYDGV
                     DTRDLSRDELRAQFSMVLQDTWLFTGSIYDNIKYGNDDATDEQIIEAAKAAHVDDFVR
                     KLPEGYNTVLNEDASNISQGQRQLITIARAFLANPDVLILDEATSSVDTRTEILIQKA
                     MNRLLENRTSFVVAHRLSTIRDADNIIVMNHGSIVETGNHDTLMEKDGFYADLYNSQF
                     SEEEAS"
     gene            complement(2202..3929)
                     /locus_tag="IXT20_001199"
     CDS             complement(2202..3929)
                     /locus_tag="IXT20_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ4859656.1"
                     /translation="MLKIVKRISLTSAIAAAVFMVIQVLADLYLPTLTSNIIDKGVAQ
                     GDVDYIWHTGFVMIGFSLISILAAIANTFFATRESQKLGKQLRTDVYKKSESLTKDAF
                     DKYGTASLITRTTNDVTQIQMVTQMFLRMMINAPITLIGASILAYQKDHQLTKIFLVV
                     IPVMIILIGGIMYFAVPLFKSMQKKTDRLNLVFREGLTGVRVIRAFDKTRFEENRFDL
                     ANKDYTNTAIKVNTIVALMMPMMTLIMSGTNVAITWFGGHYIADMTLEVGNLIAFMTY
                     AMQILISFMMLSAIFIMVPRAQASADRINEVLDEKIGIHDPENPKTVSFAGKNATLAF
                     NHVNYRYHGAEKLALEDIDFQAKSGEIVAIIGGTGSGKTTLVNLIPRLYDIESGSIQI
                     NGTDISDMTQYNLRELMGFVPQKAVLFSGTIRDNMQYGKPDATDEMIWKALEIAQAKD
                     FVSEMEDGLDSHVEQGGGNFSGGQRQRLAIARALVKTADIYVFDDSFSALDFKTDANL
                     RQALKTNMKESITVLVAQRVSTVMDADMILVLDEGKLVGKGTHEELLATNETYQEIVH
                     SQLREEDLA"
     gene            complement(3945..4595)
                     /locus_tag="IXT20_001200"
     CDS             complement(3945..4595)
                     /locus_tag="IXT20_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAQ4859657.1"
                     /translation="MPTQTFFHLPKEKQKRLIEAARIEFSRVPLKEASIANIVKLADI
                     PRGSFYQYFEDKEDLYFYYFETMRRDSSKDMLQLMKESDGDLFKGFESYFSKMIYEIL
                     EGENASFYRNLFMNMDYRASRKVAPHTSRQKTPHDPHKEHKQDAKELLELIDRTKLKV
                     KNDHELELLIQLVMNTVFTTIAHSYRLFSTSEDYSVEQAISEFNLKLSWLKNGVYK"
     gene            4799..5095
                     /locus_tag="IXT20_001201"
     CDS             4799..5095
                     /locus_tag="IXT20_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HAQ4859658.1"
                     /translation="MAQSISVQDFNKLPLDADSIVLDTRNTLDYTKNHLSHSLSIPAA
                     ILPRQLKNLDPKKLYYILSYTGRRSEALADQLTANGFRAVYIIGGMEALQSSVA"
     gene            5287..5907
                     /locus_tag="IXT20_001202"
     CDS             5287..5907
                     /locus_tag="IXT20_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HAQ4859659.1"
                     /translation="MASVLVVKGHPLTAEESRTVKALTSFLTSYKENHPDDEVTVLEL
                     YRDDIPEIDEELLSGWEALRAGAEFTSLSESQQQKIARFNELTDQFLAADKIVIANAL
                     WNLNIPTKLKAWFDTVNVAGKTFRYTENGPEGLVTGKKALHIQSNGGVYNGQDFASQY
                     VKGILNFVGIDQVDQLFIEGIDYDPDRADELMQNALDKAAALGKSF"
     gene            complement(5999..7165)
                     /locus_tag="IXT20_001203"
     CDS             complement(5999..7165)
                     /locus_tag="IXT20_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAQ4859660.1"
                     /translation="MEGKNKKNRPVSWFWRWFLNNQVVTALLVVLLILLIVFLFTKVS
                     YLFAPIWQFLAIVGLPIILAGILYYLMNPVVDYFEKRKVPRLYSIIGLFVLVVALIAW
                     GSVVIIPKIQEQTVSFISNFPQYVDTIDNKLTEILRDPLFNQFREQLETAGDKFMNSA
                     GDMIQDISKSTVQSLGSFVGAVATILVALLTMPFILFYLLKDGKQLAPYFVKFLPTRM
                     QKPTLNVLSEMNSQVSSYIRGQLTVAFAVAIMFMIGFAVIGLEYAVTLGIIAGFLNLI
                     PYLGSFLAMIPAIFLAIVAGPFMIIKVLIVFAVEQTIEGRLISPLVLGNQLAIHPVTI
                     LLVLLTSGKLFGIIGVILGIPVYAAAKVVITHVFEWYTTISSLYEENPLKKQKE"
     gene            complement(7177..7530)
                     /locus_tag="IXT20_001204"
     CDS             complement(7177..7530)
                     /locus_tag="IXT20_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HAQ4859661.1"
                     /translation="MIQAKITEIGTEAINKEEPMLILFDKTATAALRKYSVIQEVSKD
                     EPFSLKAGGTIAFDDQVYTIDYVGPMANGNLKTVAHVSLIFDACPEEGQIANGIYLHP
                     HRLPAIGLGSQIFYN"
     gene            7658..8680
                     /locus_tag="IXT20_001205"
     CDS             7658..8680
                     /locus_tag="IXT20_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HAQ4859662.1"
                     /translation="MIEKIILGTYTRRESKGIYTIDLDTEKEELANLSLAVEENSPTY
                     VAKSKAGNLYTVTSVDGLGGAGAYDKDFHFLNAVTESGAPLCYVAVDEARQLLYGANY
                     HKGEVNVYHILGDGELKAADSIFHQEATGPHKNQDHAHVHYTDLTPDQRLVVCDLGTD
                     RVYTYDVSENGKLNLVTTFTAEPGTGPRHLVFHPNGQFAYLFGELDSTVRVLSYNKET
                     GAFEELQKISTLPEEFDGENGGAAIRISNDGKFLYASNRGHNSIAVFAVFEDGSHIQN
                     KQIISTEGDFPRDFALDPSNNFVVCANQNTDNLTLYRRHPETGLLELLKKDVYVPECV
                     CVYFDR"
     gene            complement(9119..9553)
                     /locus_tag="IXT20_001206"
     CDS             complement(9119..9553)
                     /locus_tag="IXT20_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4859663.1"
                     /translation="MLPNIGYYVQSIDQIVKETEAIGEGMHPKYEVIRNAIDEGKTSE
                     LDKSTLEEIILLFEEGTKKYRAMLMKVSTLRPPAKAMGIHKNFEKAYLSYVAGCEEMI
                     QSIDLESGVDEDLFNTSEAKQDQATDQLTAAIQKMTNLLLKK"
     gene            complement(9663..11522)
                     /locus_tag="IXT20_001207"
     CDS             complement(9663..11522)
                     /locus_tag="IXT20_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HAQ4859664.1"
                     /translation="MKRYISRLSTKQKKILKGTAAIISIFFLVVFSNLFLQWCQNDLS
                     VDLALKFAFSWHTEKFFLACLVLLIILIFLIALAGSVPLGSLTYVVAIGVLGFANYMK
                     MSYRQEPIYPDDLKMITEIGLLKDMTGTMLFTVILAAAGTVIGLFCWYMFRSLKKGRR
                     FQLIRLTTLLVAIGLLGYISNFNNPDNLLRKSYNKTALWIPYSQKMNYYNTGFIGGFL
                     YNLKVEPMDEPEGYSKEKIKEITEKYQKLADEKNKTVEEESPNIVFVMSESFSDPSRL
                     NGVEVSGEPLADYYEVADQTYSGNMLSQNYGGGTANIEFEALTGFSMALFNAQLTTPY
                     TMLVPKMDQLPSIVSALNAQSYQTTAIHPYNTSMYKREDVYQTLGFDQFISERTMTYT
                     DTIENNPYISDESAYKEVMTLLKEEKTPQFIHLVTMQTHMPYDGKYDNLDYPAKTSDG
                     SGTLDLENYLQDISYSSTALKQFTEELKNLSRRTLVVFWGDHLPGIYSDTIQAKNDKQ
                     TLHETQFLMFDSKGELEKQTTHDAITSPFYFAANLMEQTNQTTNGFYQLLLSLEQELP
                     AFERELYYQNGQWYKEAQFNRSQQEIYDEYQLIQYDIVAGKQYSLADGFFEHE"
     gene            complement(11640..12799)
                     /locus_tag="IXT20_001208"
                     /pseudo
     CDS             complement(11640..12799)
                     /locus_tag="IXT20_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332175.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
     gene            complement(13106..14530)
                     /gene="araA"
                     /locus_tag="IXT20_001209"
     CDS             complement(13106..14530)
                     /gene="araA"
                     /locus_tag="IXT20_001209"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HAQ4859665.1"
                     /translation="MLNIGEKEFWFVVGSQHLYGEEALREVKKHAQEMVDELNENGQL
                     PYPIRLQELAVTADTITKIMKEVNYREEVAGVITWMHTFSPAKMWIRGTKLLQKPLLH
                     LATQYNESIPWKTIDMDFMNLNQSAHGDREYGFINARLNKQNKIVVGYWKRPEIQKEI
                     ADWMDVAVAYNESFGIKVARFGDNMRNVGVTEGDKVEAQIQFGWTVDYFGIGDLVQVI
                     DRVSDEEVDQLFEEYKELYTFDYGDYEEKTWEEHVKVQAQQEIGIRRFLEEGGYNAFT
                     TNFEDLYGMKQLPGLAVQRLMAEGYGFAGEGDWKTAAIDRLLKIMARGKDTGFMEDYT
                     YELASGQEAILESHMMEVDPTLAATKPRIVVSPLSMGDREDPARLVFDGKAGEGVVVS
                     MADFGTHYKLLINEVEAFEPTTEAPNLPVARVLWKTKPNFHEGVHSWIQAGGGHHTVV
                     SLNLTTDQIETWAKLVELETVVIR"
     gene            complement(14546..15244)
                     /locus_tag="IXT20_001210"
     CDS             complement(14546..15244)
                     /locus_tag="IXT20_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HAQ4859666.1"
                     /translation="MLEQLKEEVYQANLDLPKHGLVKYTWGNVSAFDPETRYFVIKPS
                     GVSYEEMTADDMVVVDLDNHIIEGKLNPSSDTPTHAVLYRSFPQIGGIVHTHSTWATI
                     WAQAGLDVPAMGTTHADTFYGSVPCARFLTQQEIDSGYEYETGKVIIETFKERKIDPL
                     AIPGVLLHGHGPFTWGKDAQSAVMNAVVLDEVCKMNLFTRQLNSFSEELPQRILDKHY
                     LRKHGKNAYYGQNK"
     gene            complement(15201..16862)
                     /locus_tag="IXT20_001211"
     CDS             complement(15201..16862)
                     /locus_tag="IXT20_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HAQ4859667.1"
                     /translation="MKEVNAQSMLDVIDGRTSLGIEFGSTRIKAVLIDSSFTPIAAGS
                     YEWENKLENGIWTYSLEDIWKGLRVSYRKMAAEVFEIYGENLTTIGSIGFSAMMHGYL
                     AFDSKEQLLVPFRTWRNTITEEAERILTEKFQYNIPQRWSIAHLYQSILNKEEHVKEI
                     NFLTTLAGYVHWQLTGEKVLGVGDASGMFPIDPSTKTYHQEMKNIFGQLIEKAHVAID
                     LDHILPKVLVAGEEAGKLSESGAYLIDPTGNLRPGIPVCPPEGDAGTGMVATNSVAKG
                     TGNISAGTSAFAMIVLEKELENVHPEIDMVTTPSGELVGMVHTNNCSSDINAWVSIFE
                     EFTNSLGMEISRDKLFTVLFNKALEGDTDCGGLLSYGYFSGENITGVNEGRPLFVRTP
                     GSRFDLANFMRIHLASAFGAMRIGMDILKSENVQIDRLVGHGGIFKTPEVGQRILASA
                     MEAPVTVMDTAGEGGAWGIALLAAYMMDRHGKSLENFLAEDVFGQDQGITITPSAEEI
                     AGYQIFMRRYREGIDIEKTAIHQLQEKGVIENAGTTERRSIPSKS"
     gene            17080..18480
                     /locus_tag="IXT20_001212"
     CDS             17080..18480
                     /locus_tag="IXT20_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HAQ4859668.1"
                     /translation="MKKEKKVSSSFIYFFGAFGGILFGYDIGVMTGALPFLQHDWGLA
                     GKASLIGWITSSVMLGAILGGSLSGLLSDKLGRRKMILLSALIFMAGSVLSASAPHNG
                     SYFLIAARILLGLAVGAASALVPAYMSEMAPARLRGRLSGINQVMIASGMLLSYVADY
                     LLKGLPETMAWRVMLGLAAVPALILFFGVLALPESPRFLMQSGRLEEAKRVLNYIRTP
                     KEAEQEFEQIQLNVKQEKTTGTSWHTLFLEKYRSLVIAGIGVAVFQQFQGANAIFYYI
                     PLIVEKATGHAASDALMWPIIQGIILVAGSLLFLVIADKFNRRTLLKIGGSVMGLSFI
                     LPAVLGTVLDAHTNSLLILLFLCIYVAFYSCTWAPLTWVIVGEIFPLAVRGRASGLAS
                     SFNWIGSFLVGLLFPVMTASMSQEIVFGIFGIICFLGVLFIQEIVPETRGKSLEEIEQ
                     SASKKTYPKRISIHHS"
     gene            complement(18732..19811)
                     /locus_tag="IXT20_001213"
     CDS             complement(18732..19811)
                     /locus_tag="IXT20_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ4859669.1"
                     /translation="MAKYQDIAEEIKQKILREEYEVNTTIPSEFKLQEIYDVSRHTIR
                     QAIAVLVNEGYLRKEKGSGTYVDDSYKKEKTPLNRTKTIGVITTYLSDYIFPSIIRGI
                     ENELRESGYSLLLASTNNDHELEKECLKKMIAYGVDGLIVEPTKSNQYNPNLALYVAL
                     REQGIPMVMINAAYEELNASYICLDDVKVGFMAAEYLIKKGHQRLLFITKIDDLQGKY
                     RMKGFISACEQYNVRFLTEDIITFTTETRETIAEKTVQRLKEEQALDGIICYNDQIAS
                     LMLDKLTENGYHIPDDLSIVGTDDSSLSHLGNKKLTTLTHPKEKLGQDAARWIVTTIE
                     KGQAQPNILYEPVLVERETVKENEK"
     gene            complement(19897..20856)
                     /locus_tag="IXT20_001214"
     CDS             complement(19897..20856)
                     /locus_tag="IXT20_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HAQ4859670.1"
                     /translation="MMKTLYNNPIVRQRADPWVYKHTDGYYYFTGSVPGYQVIELRRA
                     KSLNELEHAASLIVWQAHEEGPMSELIWAPEVHYINGKWYIYFAASNHKTIRDESHHH
                     RMFVLENQHIDPMNTDWIEKGQIKTKRETFSLDGTTFSSQGKLYYVWAQQDPEITGNS
                     NLYLSEMDNPWTLKGKQILLSIPEYEWEKRGFSVNEGPAVLTRNGKIFITYSGSATDE
                     NYAMGLLWADEHKNLLLKESWHKLSEPVFVSSEKNKQFGPGHNSFTVSEDGCFDILIY
                     HARPEKNQKGDPLDNPNRHANAQCFTWNEQGFPEFGEPVPYAL"
     gene            complement(20868..22391)
                     /locus_tag="IXT20_001215"
     CDS             complement(20868..22391)
                     /locus_tag="IXT20_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HAQ4859671.1"
                     /translation="MDEEVKVKIREDFTVGEISSRLFGSFVEHMGSVVYTGIYEPGHE
                     TADGDGFRQDVLSYVKELELGVIRYPGGNFTSGYDWVDTVGRKEQRPKKIDLAWRGIE
                     PNSFGLHEFFKWISHVQAKPIFTVNLGTKGIDEAKNLVEYCNFPKGTFWSDLRIKNGQ
                     KKPFGVKLWCLGNELDGPWQVGAKSAYEYGRLANEASKVMKLVDDSIETILVGSSTPR
                     LPSYPEWDRIALEEAYENVDYLALHNYIDKYDEEDLTKPPKNERDDTATYLAKAYRFD
                     RQIEEIVATCDYVKGYLRSEKTMYLAFDEWNVHAQPEKRHRDFEVGSPIDWCYFTMED
                     TLLFGSLGLAILRHADRVKISCQSLLVNTIPLILTDAKGQAWRNPTYYVMQHLSKYAK
                     GQVLKQQIICPVYDCEKIKNVPYVDSVIVDNGNQLTIFLINRTNEQQKITIEHDFNLL
                     DQGEHYTLTSSDLLDKNTREEPEKIKPEINYFSIEKHDELNVGIEKYSWNVIRIKKM"
     gene            complement(22401..22964)
                     /locus_tag="IXT20_001216"
     CDS             complement(22401..22964)
                     /locus_tag="IXT20_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HAQ4859672.1"
                     /translation="MKIVETKLYGQLIVVSDYILLGILWVIVCLPVITIVPATCAVFY
                     VMAQWKGEGSGNIIYFFFQGIKQHFGKNLFLSLLTIIIYFLVNQLLEEQTIVLSISGY
                     VVLLIYSMFLLSWIYVISENKDITFLQIFEQSAWKVFFDFLRNILCCVLLLGFVLLIF
                     LFPPFIFIFAGGVWKLVSIILTIGNER"
     gene            complement(22970..23833)
                     /locus_tag="IXT20_001217"
     CDS             complement(22970..23833)
                     /locus_tag="IXT20_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAQ4859673.1"
                     /translation="MAPESSITVTVNSRKAVSGKKIIRTISMLVILLVIAFPFLWLII
                     SSFKHEKDIISFPPRIFADSYTLDNYIKVWTTIPLLDYIKNTVIFAGGTVITSVFFDS
                     LAGYAFARMRFKGKSVLFYFVLLTMMIPFQVFMIPLFIEVNLLGMLDTYAGLIIPRMT
                     TAFGIFMMRSFFITLPDSLEEAARIDGLSEFNIFLKIMLPLSKPTLLSLGIFTLMNSW
                     NDLLYPLILTSSSKMRTLPAGLALFTGQNISFYGPVMAGTVISMLPLLVVYIFAQKYF
                     VQGTAMSGMKE"
     gene            complement(23847..24743)
                     /locus_tag="IXT20_001218"
     CDS             complement(23847..24743)
                     /locus_tag="IXT20_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ4859674.1"
                     /translation="MNGFTKYWNRPDRAAYLFLTPSFLVLLLFTVIPLLGTFAISFTD
                     LNIFFTSRNFVSFENFTRIFSDERAVNSLIHSLYFTVLETPTQIIVGLLAAVALSKNT
                     RFNRFCRSTFYIPVICSLTSIAIIFSMLLDPNVGAIPYLFSQVGLPIPQFFRDPTLAM
                     PTVAIMTVWKNFGVTMTILLTAIQGISPSLYESAQMDGATNRQQFFNITLPQIVPSLG
                     FCILTNLIGSMQVFDQVYVATAGGPQFKTETAVQYIYSRGFTAPYELGYASALSSVLF
                     IIVATIAISTNVYMSRKEKQMK"
     gene            complement(24918..26189)
                     /locus_tag="IXT20_001219"
     CDS             complement(24918..26189)
                     /locus_tag="IXT20_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAQ4859675.1"
                     /translation="MKGKKWLIGSLLVAATGILGACGGGSSAESDSDKEVTLTFWNGF
                     TASDGEILQEIVNEFNKTNDKNIKIEMDIMTWANLNEKLPTAISSKKAPDFIALNYPD
                     FAQYVENGAVQPLDDFWEYDGVDKSDFKETAIELGEVEGKQYFVPMQVQGMYMYWNND
                     LFKQAGLDTEQPPTTWEELTEMAPKLTDSSKNVSGFVFNKDGTAPLYNWMLANGGKLV
                     NDDYTKSEFASPENLKTLKAIQKMIHVDKTGPESISGAEMDNLMNAGQLAIEINGPWL
                     NNGLKANEIDYNVTTLPQGSDSDKKTAILDGVGYAIPASTDNNKKEAVYEFLKYWNTT
                     EIGKKWSIENGFPAYLDSVAKDEEVKNDPIVSELSKQMDYAEPYLPGFTQISAINNDI
                     INPLIEKLLAGEDPETLMNDADDAINQLLQK"
     gene            complement(26179..27696)
                     /locus_tag="IXT20_001220"
     CDS             complement(26179..27696)
                     /locus_tag="IXT20_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HAQ4859676.1"
                     /translation="MEATMLISSVKRVGKVDPRLYSSFIEHMGRAVYEGIYQPDHPSA
                     NKSGFRQDVIHLVKELNIPMIRYPGGNFVSGFRWEDSIGPIANRPRRLDLAWRTIETN
                     EVGIHEFYNWCQHVGAEINMAVNLGTRGVDAARNLVEYCNFPKGTYWSDLRRSNGQES
                     PFGIKTWCLGNEMDGPWQIGHKTAEEYGRLAEETAKAMKLVDDSIEVVLCGSSNRQMP
                     TFGDWELTVLDQAYDQIDYLSLHQYYGNQKNDLSHYLARSLDMDRFISEVTAMCDTVK
                     AKKHSKKQINLSFDEWNIWYHSAEQDEQAAPWQIAPPLLEDNYNFEDALLLGCLLITL
                     LKHADRVKIACLAQLVNVIAPIRTETNGEAWRQTIYYPFMQAAVYGQGEVLSPQIRSA
                     NYATEDFEEVPYLESIAVINDKELVIFAVNRGEEEMNLTVHLSELSLEGVIDFSEMSG
                     FDTKATNLPKSEQVKPSHSTNVKAEDGKINCLLKPLSWNVVRCRLSDEGGTKNER"
     gene            27894..28784
                     /locus_tag="IXT20_001221"
     CDS             27894..28784
                     /locus_tag="IXT20_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HAQ4859677.1"
                     /translation="MQLQINEESLPVYEALASKTRIKIIQLLSKKKMNVKDLAKELGV
                     SSAITTMHVKKLEEANIIKTEKVGQQKISSLRVDKIDISFPEKIFNAFDTKETSIPIG
                     HYTNYAIEPTCGLATIHDFIGKVDEPRYFMDPRRMDARILWFTSGFVEYQAPNFLNTE
                     DTLEMLEVSVEISSEFPFSNDNWPSDITFSLNGVELGTWTSPGDFADIRGKYTPDWYP
                     DNLNQYGLLKTIRITKHLTNMNGEPLSNITINDIPKEQDTWHLRIEVKDDAKHVGGCT
                     LFGKGFGNYDQDIKLKVYYS"
     gene            complement(28857..29681)
                     /gene="nadE"
                     /locus_tag="IXT20_001222"
     CDS             complement(28857..29681)
                     /gene="nadE"
                     /locus_tag="IXT20_001222"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HAQ4859678.1"
                     /translation="MSLQTEIINELGVKPTIDPKEEIRKSIDFMKAYLKKYPFLKTFV
                     LGISGGQDSTLAGRLAQLTMEEMREETKDENYQFIAVRLPYGEQADEEDAKAALDFIQ
                     PDVSLRVNIKPAVDAQVQVLSEAGVGISDFNKGNIKARQRMITQYAVAGERAGAVLGT
                     DHAAENITGFFTKFGDGGADILPLFRLDKRQGKQLLQALNAPEKLYTKIPTADLEDGK
                     PMIADEVALGVTYNEIDDYLEGKEVPAQAQEKIEAWWNKTQHKRHLPISVLDDFWK"
     gene            complement(29693..31165)
                     /locus_tag="IXT20_001223"
     CDS             complement(29693..31165)
                     /locus_tag="IXT20_001223"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HAQ4859679.1"
                     /translation="MNNTYPDDSLTLHTDLYQINMMQTYWELGRADLHAVFECYFREM
                     PFNHGYAVFAGLERLVNYLENLTFNDSDIAYLRGLEVYPEGFLEYLQNFEFKATVRSA
                     REGELVFANEPLIQVEGPLAHCQLVETALLNMVNFQTLIATKAARIKSVIGEDPLLEF
                     GTRRAQELDAAVWGTRAAYIGGADATSNVRAGKIFGIPASGTHAHSLVQSYGNDYEAF
                     MAYAKTHKDCVFLVDTYDTLKSGVPSAIRVAKELGDKINFQGVRIDSGDMAYISKRVR
                     EQLDAAGFTEAKIYASNDLDEATILNLKMQKAKIDVWGVGTKLITAYDQPALGAVFKL
                     VSIEDDEGKMMDTIKLSSNAEKVTTPGKKQVWRITRNFDGKSEGDYVTLWDEDPREEE
                     AIFMFHPVHTFINKTVRDFTARPVLQDIFIEGKRVYELPALNEIKEYTKENLDSLWEE
                     YKRDLNPQKYPVDLSTECWNHKMAIMERMKKSVAELHTEA"
     gene            complement(31802..32164)
                     /locus_tag="IXT20_001224"
     CDS             complement(31802..32164)
                     /locus_tag="IXT20_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HAQ4859680.1"
                     /translation="MKLIETPVNSNLNIKTFYPKVVEFFFGNTAINYYKLFSLDRTQL
                     LLVDTYDKKQVVMINTKKKITRQEIDYAIHHVLKMTREDVKVHVGVKQELERAGIQFK
                     RPNKDIVVIEQKNTKDSI"
     gene            32387..33139
                     /locus_tag="IXT20_001225"
     CDS             32387..33139
                     /locus_tag="IXT20_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAQ4859681.1"
                     /translation="MEYTIKKMSEISGVSPRTLRFYDEIGLLKPARINSSGYRIYGKK
                     EVDRLQHILFYRTMAFKLDDIQEVLDNPSFDHQKALIKHQQMLLEKRAQIDTLLTTVQ
                     QTLDMYEGGRKMSDKEKFEGFKQQKLKENEESYGQEIRQKYGKKKVTASNEKWMNMDK
                     ETYEEMQTVEKQMLFDLTLYLKEPEDESLADRIFQAHKKWFSYSWPEYQPKAHKGLGM
                     MYEADERFTAYYDERSGHGAAKALNEIIQKKA"
     gene            complement(33279..34202)
                     /locus_tag="IXT20_001226"
     CDS             complement(33279..34202)
                     /locus_tag="IXT20_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAQ4859682.1"
                     /translation="MANIRDIAKLTGYSVSTISRVINGHPYVDEEKRKEILAVMKEVD
                     YIPNANARQLSYGKTKSIGVILPYTNHPYFDQLISGIIEAAFDYDYKVTLLPTGYHKE
                     KERRYLEEFAAKAFDGLIITSRANTLDDLLAYQKYGPLVFCEEIKGKQASCVFIDREQ
                     SITEGLTYLKQQGIKQLGITLGRSGRLSYNSKITLQLCHQLFPSFDESLVFWDCIDAE
                     KGKQAGFFFKELGVDGVFTNSDMVAAGILQSYLQDKTPVVIGRDNLLISELLGFPTID
                     HHLEACGEMAFQLFLTEKKDKVKIPYTFIQR"
     gene            complement(34217..34489)
                     /locus_tag="IXT20_001227"
     CDS             complement(34217..34489)
                     /locus_tag="IXT20_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4859683.1"
                     /translation="MNTYVEITDGTTNNYFYAFVEFKGSVLTLYSFQGLNRNIVKEIP
                     MNEIENLTKDIYWGGQRISFSHEGKMYQFFECGPAVVDYLQENLFV"
BASE COUNT        10230 a         7211 c         5981 g        11239 t
ORIGIN      
        1 caagaggttg tgagcctgcc gtttaactcc gagtcacaag gagcaatttc aaaaaacagc
       61 ttctcaggtt tgttgactat tgtgacttgt gaccgaggag ttggcaggag aacaccgttt
      121 aaggaagagg ttgtaaaatc agcggtttga cctgagtatt agagatacaa aacggaaaaa
      181 tagcttctca tattttgccg ttttttgagc taataccgaa ggacagctga ttgaacaccg
      241 tttattcgga aaaagggact gtgacacgac ttttgtcaca gcccctaaaa aaaataataa
      301 atttgtacta ttctatgaag cttcttcttc agaaaactga ctgttataaa gatctgcata
      361 gaatccgtct ttttccatca atgtatcgtg gtttcctgtt tctacgatcg aaccatgatt
      421 catcacaata atgttgtctg catcgcgtat tgtagacaag cggtgagcaa caacgaaact
      481 tgtccggttt tccaataggc ggttcatcgc tttttgaatc agtatttctg ttcgtgtatc
      541 tacactagat gttgcttcat caaggatcag gacatctgga tttgctaaga atgcccgcgc
      601 aattgtgatt agctgacgct gcccttgcga aatattgctt gcatcctcgt ttaatacagt
      661 gttatatcct tctggcaatt tacgaacaaa atcgtctacg tgagcagctt tagcagcctc
      721 aatgatttgt tcgtctgttg catcgtcgtt cccatatttg atattgtcat agatacttcc
      781 tgtaaacagc caagtatctt gcagtaccat tgaaaactgt gcgcgcaatt catcacgtga
      841 aagatcacgg gtatctacac catcgtaacg aatactacct ccagaaacat cgtagaaccg
      901 ttcaagaagg ttgatcagtg tcgtcttccc tgcaccagtc ggacctacaa tagcgaccat
      961 ctcaccaggt ttgacatcca agttaaaatc agtcattagc aagttatctg gtgtgtagcc
     1021 gaattgaaca ttttcaaaac gaactttgta tggtgtatct ttttcttctg gaaggccaga
     1081 tttttcattg gacatctctt cttcatccaa cacttcaaat acccgttcgg ctgaagcaac
     1141 tgttgattgg atagtgttca tcaaacttgc aagttgagtg attggttgag agaactgtgt
     1201 tgtatattgc aagaacgctt gaacatcccc taatgtcatg ttcccatttg caacttttac
     1261 tccgcctagt acagcgacga acacatagcc tagattttta acgaaattca tcaacggcat
     1321 gatgattgcc gaaataaatt gagctttcca accagcggca tacagattct gattttcttt
     1381 ttcaaatact tcctggtctt tttcagtatg attgaatgtt ttaacaatgg tatgaccacc
     1441 atatgtttct tctacttggt tattcaataa acctaaactt ttttgctgat ctgcgaaaaa
     1501 cacttgtgat ttcggcgcta cgatcatcac gatgatcaag cttaaaggga tcatcaataa
     1561 cgcgactaac gtcaattgcc agctgattga aagcatcatc acgatgaccc caatcaatgt
     1621 gactgcacta gttacaaatt gagtcaaatt ttgctgcagc gtgcttgcga tgttatccat
     1681 atcattgatt gctcgagaca tgatatcccc atttgtatgt gtatcataat aggaaatcgg
     1741 tacacgattc attttggctt ccaaatcatg acgtaattca taaactgttc gttgggaaac
     1801 acgtgtcata ataaattgct gtaaaaagtt gaatacagca gaaatcaaat acatcacgat
     1861 tactgtcgca atgatctgcg cgattttatc aaagtctatc ggaaattttg ttacttgttg
     1921 gccttgttgc atctgttgag cgccagccat tacacctttg aatatttctg tggttgcttt
     1981 ccccaagacc tttggtgttt ggatttggaa gatcacggct gctattgcta gtacaaatac
     2041 agcgatgatc gcaaccatac gtttagacat atagccaaac aatcggcgta ccgttcccca
     2101 gaaattttta ggcttttcac cttttacacc gagatttctg ccaggtccac catgtcctgg
     2161 accgcctgct ggtccatgag agggacgtgt gtttgtttta ctcatgctaa gtcctcctct
     2221 cttagttgtg aatgaacgat ttcttgataa gtttcgtttg ttgctaatag ttcttcatgt
     2281 gttcctttgc caaccagttt tccttcatct agcacaagga tcatatctgc atccattact
     2341 gtacttactc gttgagcaac taaaacggtg atactttctt tcatattcgt tttcaatgct
     2401 tggcgaagat tggcatccgt tttgaaatcc aacgctgaga aggaatcatc aaacacatag
     2461 atatctgctg ttttgactaa tgcccgtgca attgccagac gctgtctttg tccaccagag
     2521 aagtttcctc ctccttgttc cacatggcta tctaagccat cttccatctc tgaaacaaaa
     2581 tctttagctt gtgcgatttc taatgctttc caaatcattt catctgttgc atccggctta
     2641 ccatactgca tattgtctcg gatcgttcca gagaacagaa ctgctttttg aggaacaaag
     2701 cccattaatt ctcgcaaatt atattgagtc atgtcactaa tgtccgttcc atttatttgg
     2761 atcgaaccag attcgatgtc atataatcga gggatcaaat tgactaaggt cgttttacct
     2821 gaaccagtcc cccctatgat tgcgacgatc tcgcccgact tggcttggaa atcaatatct
     2881 tccaatgcaa gtttttctgc tccatgatat cgataattga catgattgaa ggccaatgtt
     2941 gcgttttttc cagcaaaaga aactgtttta ggattttcag ggtcatggat accgattttt
     3001 tcatccaaca cttcattaat acggtcagct gaagcttgtg cacgaggcac catgataaaa
     3061 atagcggaaa gcatcataaa gctgatcaag atctgcatcg cataagtcat aaatgcaatc
     3121 agattaccga cttccaaagt catatctgct atgtagtgtc caccaaacca agtgattgct
     3181 acgtttgtac cgctcatgat caatgtcatc attggcatca tcaatgcgac gatcgtatta
     3241 actttgattg ccgtatttgt ataatcttta ttcgctaaat caaaacgatt ttcttcaaag
     3301 cgtgtcttat cgaatgctcg aatcactctg acccctgtca atccttctcg aaataccagg
     3361 tttaaacggt cagttttctt ttgcatactt ttgaataatg gaacagcaaa gtacatgatt
     3421 ccgccgatca aaataatcat tactgggatg acaactaaga agatttttgt caactgatga
     3481 tctttttgat aagcaagtat gctcgcgcca atcaacgtga ttggtgcatt gatcatcata
     3541 cgtaaaaaca tttgtgtaac catttggatt tgagtcacat cattggtcgt tcttgtgatc
     3601 aaagaggccg ttccatattt atcaaacgca tcttttgtca gactctctga ttttttatag
     3661 acatccgtac gcaattgctt acccagtttc tgtgattcac gagtagcaaa aaatgtattt
     3721 gcgatagcag ctaagatact gatcaaagaa aagccgatca tcacaaaacc tgtatgccaa
     3781 atataatcga catctccttg cgctacccct ttatcaataa tattggaggt aagtgtcggc
     3841 aaatataaat ctgccagtac ttggatcacc atgaaaactg cagctgcaat tgctgaagtc
     3901 aaggagatcc gttttacgat ttttagcatc tagttccctt ctttctattt atatactccg
     3961 tttttcagcc aagaaagttt caaattgaat tcactgattg cttgctctac tgagtaatct
     4021 tcagatgttg aaaataagcg atacgaatgc gcaattgttg taaatacagt attcatcact
     4081 aattggatca gtaattccaa ttcatgatca ttttttactt tcagcttcgt ccgatcgatc
     4141 aattcaagca actcttttgc atcttgtttg tgttctttat gtggatcgtg aggcgttttt
     4201 tggcgactcg tatgaggagc gacttttctt gaggcacggt aatccatgtt cataaacaag
     4261 ttacgataaa aagaagcatt ctcgccttcc aaaatctcat aaatcatttt agaaaaatag
     4321 gattcgaatc ctttgaatag atcaccatcg gattctttca ttagttgtaa catatcttta
     4381 gagctgtcac gtctcattgt ctcaaagtaa taaaaataaa gatcctcttt atcttcaaaa
     4441 tactgataga aactgcctct agggatatcc gctaatttga cgatattagc aatcgaagct
     4501 tcttttaagg gtactctaga aaactcgatt ctcgcagcct cgattagtct tttttgtttt
     4561 tctttaggca aatggaaaaa tgtctgtgtc ggcatatgga ttctcctttc tttcaagatg
     4621 acacattgtc atatgacgct atgtcattat aacacctcct acctgttttg caagacattt
     4681 gcatagtttt tttcattatc atccgataca ttaaaaaagt gataagagga atccttccat
     4741 ttgaattaat gagagttgcc cattaaaata gccacatact aaacaaagga tgagataaat
     4801 ggcccaatca attagtgtcc aagacttcaa taaattgcct ctcgatgctg acagcatcgt
     4861 tttagataca agaaatacct tagattacac aaaaaaccac ttaagccact cgctttctat
     4921 ccccgctgct atcttgccaa gacaattaaa aaatctagat ccaaagaaac tttattatat
     4981 tctaagctat acaggcaggc gatcagaagc attagccgat caacttactg ctaatggttt
     5041 tcgtgctgtt tatatcattg gtgggatgga agctcttcag tcgagtgttg cttgaatatt
     5101 gcttgttaga ttcaaaagag gctgtgacaa aaaatcgtgt cacagcctct tttataccct
     5161 gtcttttttt agttatttat tcttcaacct tatcaatatg acttgatagc cttttctttt
     5221 tcgagtaaac tgattttgtt agtcatagtt ataacaatca tagcaataaa aggagaaggt
     5281 aagcatatgg catctgtttt agtagtcaaa ggtcatcccc taactgcaga agaatcgcgc
     5341 acagttaagg cattgacgtc ttttttaaca agttataaag aaaatcatcc agatgatgaa
     5401 gtgactgttt tggaacttta tcgcgatgat attccggaaa tcgatgaaga attattatca
     5461 ggttgggaag cattacgtgc tggtgcagaa ttcacttcct tatcagaaag tcaacaacaa
     5521 aaaattgctc gtttcaatga attgactgat cagttccttg ctgctgataa aatcgttatc
     5581 gcaaacgcct tatggaatct aaatatccca acaaaattaa aagcttggtt cgataccgtc
     5641 aatgtagctg gaaaaacgtt ccgctataca gaaaacgggc cagaaggatt agtgacaggt
     5701 aaaaaagcac tgcacatcca atctaatggc ggtgtataca acggacaaga ctttgcctct
     5761 caatatgtaa aaggtatttt aaactttgta ggcatcgatc aagtagatca gctattcatc
     5821 gaaggaatcg attatgaccc tgaccgtgca gatgagttga tgcaaaatgc attagataaa
     5881 gctgctgctt taggtaaatc attttaaaaa ttgagataaa tgcatacaaa agagacagag
     5941 gaaaaaatct tcaaagcagc atttgtgcct tggatatttt cccatctgtc tctttttgtt
     6001 attctttctg ctttttcaac ggattttctt cataaagaga agagattgtt gtgtaccatt
     6061 caaaaacatg agtaatcact actttagctg ctgcataaac aggaatcccc aagatcacac
     6121 cgatgattcc gaacaatttc ccagaagtca gcagaactaa taaaatcgtt actggatgga
     6181 ttgctaactg attccctagt actaatggag agatcaagcg tccttctatt gtctgctcta
     6241 ctgcaaaaac gatcagtact ttgatgatca tgaatggacc tgctacaatg gctaaaaaga
     6301 tagccggaat catcgctaaa aatgatccta agtaagggat caggtttaaa aaacccgcaa
     6361 taatccccaa cgtgacagca tactctaaac caatgaccgc aaaaccaatc ataaacatga
     6421 ttgccacagc aaaagccaca gttagttgtc ctcttatata agaagaaact tgactattca
     6481 tttcactcag cacattcaac gtcggttttt gcatgcgtgt cggtaaaaat ttgacaaaat
     6541 aaggtgctaa ttgtttaccg tctttcaata gataaaataa gataaacggc atcgtcagca
     6601 gagccactaa aatagtcgct accgcgccga caaaacttcc aagactctga actgtactct
     6661 tagatatatc ttggatcata tcgcctgccg aattcatgaa cttatcacct gctgtttcca
     6721 actgttcgcg aaattggttg aacagcgggt cccgcaaaat ctcggttaat ttattatcaa
     6781 tcgtatctac atattgaggg aagttactga tgaagctcac tgtttgttcc tgaatcttag
     6841 ggatgatcac tacgctcccc catgcgatta gtgcaacaac caaaacaaac agcccaatga
     6901 tactgtaaag ccttggtact tttcgctttt caaagtaatc aactaccgga ttcatcaagt
     6961 aatacaagat tcctgctaaa atgataggca gacctacgat tgctaaaaat tgccagattg
     7021 gtgcaaacaa atatgaaact tttgtaaata aaaagacgat cagcaatatt aacaatacaa
     7081 caagtaaggc tgtgactact tgattattta aaaaccagcg ccaaaaccac gataccggtc
     7141 tgttcttctt attttttcct tccataattc ctcctgttag ttataaaaaa tttggcttcc
     7201 caaaccaata gcaggcagtc tatgcggatg aagatagatt ccatttgcaa tttgtccttc
     7261 ttctggacaa gcatcaaaaa tcaatgatac gtgagctact gtctttaaat tcccattggc
     7321 catcggaccg acataatcga tcgtatatac ctgatcatca aaagcaattg ttccgcctgc
     7381 tttcaaagaa aaaggttcgt cttttgatac ttcttgaatc accgaatatt ttcgtaaggc
     7441 agctgttgct gttttatcga acaaaatcag catgggttct tccttgttta ttgcttctgt
     7501 cccaatttct gtaatctttg cctgaatcat tgcactctct cctttaattg ctttcaaaaa
     7561 cagtataaca cattctcatt ctttagccgt gtggaataat tatgtgatag aataaaaacg
     7621 aaaactattt cattacaatt aggaggaatc agctcatatg atcgaaaaga taattttagg
     7681 aacctatacg cgtcgcgaaa gtaaaggaat ctatacgatc gaccttgata cagaaaaaga
     7741 agaattagcc aacctttcat tagctgttga agaaaatagc cctacctatg tagcaaaaag
     7801 taaagctggc aatctttaca cagttactag tgtggatgga ttaggtggtg ccggtgcgta
     7861 cgataaggat tttcattttt tgaatgcggt aacagagtct ggcgctcctt tgtgttatgt
     7921 agcggttgat gaagcacgtc aattactata cggggcgaac tatcataaag gtgaagtaaa
     7981 tgtctaccat atattaggag atggcgaact gaaagcagca gattcaatat tccatcaaga
     8041 agcgacaggt cctcataaaa accaagatca tgcgcatgtc cactatacgg atctgactcc
     8101 tgaccaacga ttagtcgtat gtgatcttgg tacagatcgt gtgtatactt atgatgtgtc
     8161 cgaaaacggc aaattgaatc ttgtaactac cttcactgca gagccaggaa caggccctcg
     8221 ccatcttgtc tttcatccaa atggacaatt cgcttattta tttggtgaat tagacagcac
     8281 agtccgcgtg ctttcttata ataaagaaac aggtgctttt gaagaattac agaaaatatc
     8341 gaccttgcca gaagaattcg acggtgaaaa tggaggggca gctatccgca tttcaaatga
     8401 tggcaagttc ctttatgctt ctaaccgagg acataactcg attgctgtat tcgcagtttt
     8461 tgaagatggt tcccacattc aaaacaagca aatcatctca acagaagggg attttccccg
     8521 cgatttcgca ctagatccaa gcaataattt tgtcgtgtgt gccaatcaga acaccgataa
     8581 tctgacatta tatcgccgtc atcctgaaac aggattactg gaattactga aaaaagatgt
     8641 ctatgtacca gaatgtgtct gtgtttattt tgatcgttag taagaaaagg agaatgaaat
     8701 gaatcagcaa gacgtattag acagactacg ggaagaacta aatattcctt tctttgatgg
     8761 gaaaatcgaa gataaagagt atagcgaaga ggaatatcag aaactcaaag ccgatttaca
     8821 gaactacttt gaacaatatg tacagaatgt agaaaactaa tcagctttcc atactatctg
     8881 aaaagaacat caggacaaga cttttccgtc tatcctgatg ttcttttcta caaataaaaa
     8941 accgccgaga tggttagact cgacggaaaa ggagtattta ctcgattttg agtaagtaca
     9001 aggttgttgt tggttatagg tatgtcatta tcataaagga ttttgatcac tctatcataa
     9061 aggtaatatt acatttatat tagataactc tagtttttat tgttatcaaa gatttctttt
     9121 attttttcaa caacaaatta gtcatttttt ggatggcagc tgtcaattgg tctgtcgctt
     9181 ggtcttgttt tgcttcagat gtattgaata gatcttcatc tacccctgat tcaagatcga
     9241 tactttggat catttcttca cagcctgcta catatgaaag ataagctttt tcaaaatttt
     9301 tatgaatgcc cattgctttt gctggtggac gcaaagtact gactttcata agcattgccc
     9361 gatatttttt tgttccttct tcaaataaca ggatgatctc ttcaagagtc gatttgtcca
     9421 gttcacttgt ttttccttca tcaattgcgt ttctgatcac ttcatatttc ggatgcattc
     9481 cttctccgat tgcttctgtt tccttgacga tttgatcgat ggactgcaca taatagccaa
     9541 tattcggtaa catgttcgtt ttcctacttt ctccattgtt gttattattt attctacttg
     9601 tttttatcct caaatgacaa gtacttgaaa aaatttaatg gtttctatac agctcgccat
     9661 tcttattcgt gctcaaagaa accatctgct aaactatatt gtttccctgc gacaatgtca
     9721 tattggatca attgatattc gtcgtaaatc tcttgttgcg agcgattgaa ttgcgcttct
     9781 ttataccact gtccgttttg atagtacagt tctcgttcaa acgccggaag ttcctgttct
     9841 aatgataaaa gcagctgata aaagccattt gttgtctgat tcgtctgttc cattagattc
     9901 gctgcaaaat aaaacggact ggtgattgca tcgtgagtcg tttgcttttc taattctcct
     9961 ttactgtcga acataagaaa ttgtgtttca tgcaatgttt gtttgtcatt ttttgcttgg
    10021 atcgtatctg aatagatccc tggaagatga tccccccaaa aaacaactaa agtccttcta
    10081 gaaagattct tcaattcttc cgtgaactgc ttcagtgccg tgctgctata ggaaatatct
    10141 tgcagataat tctctaagtc aagtgtccca cttccatctg aagtttttgc aggatagtct
    10201 agattatcat actttccatc gtagggcata tgtgtctgca tcgtgactaa atgaatgaac
    10261 tgaggtgttt tttcttcctt gagaagtgtc attacttctt tgtaagctga ctcatcagaa
    10321 atatatggat tattctctat cgtatccgta taggtcatcg tacgctcgct gatgaactgg
    10381 tcaaatccta atgtctgata aacatcctct cttttataca tcgaagtatt gtatggatgg
    10441 atcgctgtcg tctggtatga ttgtgcgttt aacgctgaaa cgatagaagg aagctgatcc
    10501 atttttggta caagcatcgt gtaaggggtg gtgagttgcg cattaaataa cgccatagaa
    10561 aaaccagtca atgcttcaaa ttcgatattt gctgttccac cgccatagtt ttgagaaagc
    10621 atattgccac tatatgtttg atcagctact tcataataat ctgccaatgg ctcaccagat
    10681 acttctacgc cgtttaatcg actaggatct gagaaacttt cactcatcac gaacacgatg
    10741 ttcggcgact cttcctctac agttttattt ttttcatcgg ctagcttctg gtatttttca
    10801 gtgatttctt tgatcttttc tttcgaatat ccttcaggtt catccattgg ttctaccttc
    10861 agattgtaca aaaacccacc gataaaacca gtgttatagt agttcatttt ctggctgtaa
    10921 ggtatccata gtgccgtttt attatatgat tttcgcaaca aattatcggg attgttgaaa
    10981 ttactgatat accctagcag tccaattgcc acaagcagtg tcgttaatcg gatcaattgg
    11041 aatcgccttc cttttttcag gcttcgaaac atgtaccagc aaaacaatcc tatcacggtt
    11101 cctgcggcag ctaagatcac tgtaaacagc atagtgccgg tcatatcttt cagcaatccg
    11161 atttcagtga tcatcttcaa atcgtctgga taaatcggtt cttgccgata actcattttc
    11221 atataatttg caaaacctaa tacaccgatg gctacgacat acgtaaggga acctaaaggg
    11281 acagatcctg ctagtgcaat cagaaaaata agaataatca gcaagacgag acaggctaag
    11341 aagaattttt ccgtatgcca agaaaaagca aatttcaacg caaggtctac ggatagatca
    11401 ttttgacacc actgcaaaaa taaattacta aaaacaacaa gaaaaaagat actgatgatt
    11461 gctgctgttc cttttaaaat ctttttctgt ttggtcgaaa gtctgcttat atatcttttc
    11521 aaatgcttgc tccttcattt ctatcaataa aagaatgttg cttctcttat tttagttgta
    11581 cgaaattcga aagtaaagaa cggaaagaag aattaatgaa atccaaatat tctctttctt
    11641 tatggccttt ctggtttctt taataagcgg acctttcgta ctccagaaat tttttctagc
    11701 tgttgtttca cttgattgag ctgttcaaaa tcagtactgt ctatgtcaat caatatgtag
    11761 acatgctcat tcttatggtt acgcgatata tctgcgatcg ctagctcgtt ctcattcagc
    11821 agagaaaata tctctgctaa tatattggtt ttcttttgat aaaatatagt gaatcgaaac
    11881 ggcgcttgga aaaacattcg tgcggatgga taattgaccg cttctctaac cgtaccgaac
    11941 aaaagataat cttttaaggc ttcaactgcc aaacgtccgc tgtctgccaa agcttcctga
    12001 gtcgatcccc tagatgaggc aacataagta tacggtcatt ttctaagaac tcttcttctg
    12061 gaaaatcagt caagtattta gccagatgtt gtttttccaa tgcttgcata acagcatatt
    12121 tatcaacaat ttcacttctt cctacattga taagtacagc atcttttttc attttttcta
    12181 tattcgcctg atcgatcaat ccttttgtat cttctgtcaa cggcagcatg actacgatgt
    12241 aatccgaggt agaaagaact gtttctaaat ctgcttgccg aacataatcc aactgggcat
    12301 cgcggataga ataacccaag acatccatcc ctaagctata gcaacttagc gctacttttg
    12361 tgcctatcgc tccaagacca agaagaccga tggttttgcc ttgcaattct cgtcccacat
    12421 aagcacttct tttgttttct gcctgttcta aaatattagg acctgttagt gtttgtacca
    12481 tacgacttgc ttcgattatc ggacgggaag ataagagcaa acagcacaaa acaagctctt
    12541 ttactgcatt agcatttaca cctggtgtat tcatgacgat cgtcccgttt tcagaagctt
    12601 tttcaacgtt gatcgtgttc accccaacac cagctcttga aattgctagc aaatctggtg
    12661 taagccattc atctttcact ttcgtactac gaataactat tccttcggct ggcaaatcat
    12721 ctattatttt gaaatattcc ccttgtaact tttctatctc ttgttctgga atagcatcaa
    12781 tcaaatgaac gtgatacata aaaccccctc cttactaaca agtatctcga aatttctgca
    12841 attagacaac tgtttaccat ttgacttcct agaagcaaaa agagcagtta gagcagagta
    12901 ggctgagtga taaaaataag gctatgacaa gttactgcca taagccttat tttcgagagc
    12961 aaaatattga gggtgagata tcttgataaa tggtgttccg ttagctgaca ttgataataa
    13021 gactaaattt caaaaaagtt gaaaagcaaa tttcgtcaat ctcttcttat ttcttaaaac
    13081 taagtggctt tttcacaacc tctttttatc taataaccac tgtttccaac tcaactaatt
    13141 ttgcccacgt ttcaatttga tctgttgtta gatttagcga gacaacggta tgatgaccgc
    13201 cacctgcctg aatccatgaa tggacacctt catggaaatt cggtttagtt ttccataaca
    13261 cacgtgcaac aggtaaatta ggtgcttcag tcgttggttc aaaggcttct acttcattga
    13321 tcagcagttt ataatgcgta ccgaaatctg ccatcgatac gactacccct tctcctgctt
    13381 tcccatcaaa tacaagacgc gccggatctt ctctatctcc cattgataaa ggagaaacaa
    13441 cgattcttgg tttcgtcgca gcaagcgtcg gatctacttc catcatatgg gattccaaga
    13501 ttgcttcttg gccactagct agctcgtaag tataatcttc catgaatcct gtatctttcc
    13561 ctcgagccat gattttcagc aaccgatcaa tcgctgcagt tttccagtcg ccttccccag
    13621 caaaaccgta tccttctgcc atcaaacgtt gtacagctag tccaggtagt tgtttcattc
    13681 catacaggtc ttcaaaatta gtcgtaaaag cgttatagcc tccttcttct aagaaacggc
    13741 gaataccgat ttcctgctga gcttgtactt ttacatgttc ctcccatgtc ttttcttcat
    13801 aatcaccata gtcgaaggta tacaattcct tgtactcttc aaataattga tcgacttctt
    13861 cgtctgatac gcgatcgatt acttgtacaa gatctccgat accaaaataa tcaacagtcc
    13921 aaccaaattg aatctgtgct tccactttgt ctccttctgt gacacccacg tttctcatgt
    13981 tgtcaccaaa acgagccact tttattccaa aactttcatt ataggctact gcaacatcca
    14041 tccaatcagc aatctctttt tggatctcag gtcgtttcca gtagccaacg acgattttat
    14101 tttgtttatt taatcgagca ttgatgaacc catattcgcg gtcaccatga gcggattgat
    14161 ttaaattcat gaaatccata tcgatcgttt tccaaggaat gctttcgtta tattgcgtag
    14221 ctaaatgaag caatggtttt tgtagaagct ttgtcccgcg gatccacatt tttgctggtg
    14281 agaaagtatg catccaagtg atcacacctg ctacttcttc tcgataattg acctctttca
    14341 tgatttttgt gatcgtatca gcagtaactg ctaattcttg aaggcggatt ggataaggca
    14401 attggccatt ttcattcagt tcatcgacca tttcttgtgc atgtttcttt acttctcgca
    14461 gtgcttcttc cccatataaa tgctgtgatc ctacaacaaa ccaaaattct ttttctccaa
    14521 tattcaacat aattagccac tccttttatt tgttttgtcc gtaatacgca ttttttccgt
    14581 gttttcgtaa ataatgttta tctaaaatcc gttgaggcag ctcttctgaa aatgaattga
    14641 gctgtctggt aaataaattc atcttgcata cttcatctag tacgactgcg ttcattaccg
    14701 cactttgcgc atctttcccc caagtaaatg gaccgtgtcc gtgtaaaagt acccctggaa
    14761 ttgctaaggg atcaattttg cgttctttga atgtttctat gataactttt cctgtttcat
    14821 attcgtaccc gctgtcgatt tcctgttgtg tcaaaaatcg agcacaaggt accgatccat
    14881 aaaaagtatc tgcatgcgtc gtccccatcg caggaacatc caatcctgct tgtgcccaga
    14941 tcgtcgccca tgtcgagtga gtatggacga ttcctccgat ttgaggaaat gaacgatata
    15001 aaacagcatg agtaggcgta tccgaagagg gattgagttt accttcaatg atatgattgt
    15061 ccaagtccac aacgaccatg tcgtctgctg tcatctcttc ataactgact ccgctaggtt
    15121 tgataacgaa atatcgtgtt tcaggatcaa aagcgctcac atttccccat gtatatttga
    15181 cgagtccgtg tttaggcaag tcaagatttg cttggtatac ttcttctttc agttgttcca
    15241 gcattttcta tcactccttt ttcttgcaat tgatgaatcg ctgttttttc aatgtcgatc
    15301 ccctcacggt aacgtctcat aaatatctga tagcctgcta tttcttctgc tgaaggtgtg
    15361 atggtgattc cttggtcttg tccaaacacg tcttctgcca agaagttttc taaagacttc
    15421 ccatgtcgat ccatcatata ggctgctaat aacgcgattc cccacgctcc accttctccg
    15481 gctgtgtcca tcactgtaac tggtgcctcc atagcagatg ctagaatccg ctgtccaact
    15541 tctggtgttt taaaaatccc cccatgcccc actaaacgat caatttggac attctcagat
    15601 ttcaatatat ccattccgat tctcattgca ccaaaagcac tggctaaatg gatccgcatg
    15661 aaattcgcta aatcaaaacg gctacctggt gtacgtacaa ataatggcct tccttcattt
    15721 actcctgtaa tgttttcacc ggaaaaataa ccataagaaa gtaatccccc gcaatccgta
    15781 tcgccttcta gcgctttgtt gaataatacc gtgaataatt tatctctgct tatctccata
    15841 cccaaagaat tggtaaattc ttcaaaaata ctgacccaag cattgatatc cgaagagcag
    15901 ttattcgtat gaaccatgcc tactagttct cctgaaggtg tagtgaccat gtcgatttcc
    15961 ggatggacat tttctagttc tttttctaat acgatcatag cgaaggcgga agtaccagcc
    16021 gaaatgttcc ctgtcccttt ggctacactg ttcgtagcga ccataccggt tcctgcatcc
    16081 ccttctggcg gacaaaccgg gattcctggt ctcaaatttc ccgtagggtc gatcagataa
    16141 gcccctgatt cgcttaactt gccagcttct tctcctgcta ctagaacttt aggcaaaatg
    16201 tgatccaagt caatcgctac gtgcgctttt tcgattaact gaccaaaaat atttttcatt
    16261 tcttgatggt aggttttcgt tgaaggatcg ataggaaaca tgcctgatgc atctcctacg
    16321 cctagtactt tctcaccagt aagctgccaa tggacataac ctgcaagcgt tgtcaaaaaa
    16381 ttaatttctt tcacatgttc ttctttattc aaaatagatt gatacaaatg agcgatactc
    16441 catctctgcg gaatattgta ttgaaatttt tccgttaata ttcgttctgc ttcttcagtg
    16501 attgtatttc gccaagtacg aaatggtact agtaactgtt ccttgctatc aaatgctaga
    16561 taaccgtgca tcattgcact gaacccaatc gacccgattg tcgtaagatt ctcgccatag
    16621 atttcgaata cttctgctgc catttttcgg taactgacgc gcagtccttt ccaaatatct
    16681 tctaatgaat aggtccagat accattttct agtttgtttt cccactcata actaccagcg
    16741 gcaataggag taaaagatga atcaatcaat acagctttga tcctagttga tccaaactca
    16801 atccctaaag atgtacgacc atctatgaca tctaacatac tttgcgcatt tacttccttc
    16861 ataatgattt aactccctcc ttctcgtcaa acctgatcaa accgtctttt tagtagcgct
    16921 tacaatgatt attgtatact tttgtacgta catgtcaagc agttatttac aagaatacaa
    16981 taaaaaacac ttgttttgta cgtacataag ttataaaatg aaattgctta caaaatctga
    17041 tcaaccgcct tctcgaaatt ctagaaggag agaatactta tgaaaaaaga aaaaaaagtt
    17101 tcctcatcgt ttatttattt ttttggcgca tttggcggca ttctatttgg atatgatatt
    17161 ggtgttatga ccggcgcctt acctttttta cagcatgatt ggggtttggc aggtaaagct
    17221 tctttgatag gatggatcac gtcttcggtc atgttaggtg cgatccttgg cggctcttta
    17281 tctggtcttc tttctgataa gcttggacga cgaaaaatga ttcttctttc tgcacttatt
    17341 tttatggctg gttctgtcct ttctgcatct gcccctcaca atggttctta ttttctgatt
    17401 gcagcaagga ttcttctagg tttagctgtt ggcgctgcat ctgccctagt tcctgcttat
    17461 atgtctgaaa tggctcctgc tagactaagg ggaagattgt cagggatcaa tcaagtgatg
    17521 attgcttctg gcatgctcct ttcctatgtt gcagattatt tgctaaaagg gttgcctgaa
    17581 acgatggctt ggcgcgtgat gttagggctc gctgctgtcc ccgcgctgat tttatttttc
    17641 ggtgttttag ctctaccaga gtcaccgcgt tttcttatgc aatctggccg cttggaagag
    17701 gcaaaaagag tattgaacta tattcggaca ccaaaagaag ctgaacagga attcgaacaa
    17761 atccagctga atgtcaaaca agaaaagaca accggaactt cttggcatac tttgttttta
    17821 gaaaaatatc gttccttagt cattgccggt attggtgtag cagtattcca gcaatttcaa
    17881 ggagctaatg ctatttttta ctatattccg ctgatcgtag aaaaagcgac tgggcatgca
    17941 gcgagcgatg cattgatgtg gccgatcatc caaggaatca ttttagttgc tggttctttg
    18001 ctctttttgg ttattgcaga caagttcaat cggcgaactt tattgaaaat aggcggtagc
    18061 gtgatgggac tttctttcat cctaccagct gttttaggaa ctgtactgga tgctcataca
    18121 aattcgttgt tgattctctt gtttttatgt atttatgtcg cattttattc ttgcacatgg
    18181 gctccactga catgggtaat cgtaggcgaa atattcccac tagctgtccg cgggcgagca
    18241 tctggcctag cctcttcttt taattggatt ggttctttcc tagttggctt gctttttcct
    18301 gtgatgactg cttctatgtc tcaagaaatc gttttcggca tttttgggat catctgcttt
    18361 ttaggggttt tatttattca agagatcgta ccagaaactc gtgggaaatc tttagaggaa
    18421 atcgaacaaa gtgcatctaa aaaaacttat cctaaacgta tttctatcca tcattcttaa
    18481 agaggttgtg acagaagtgg tcagcttcaa gaaataagaa ggaaccgctt agtttccagt
    18541 cacaaggaac aatttcaaaa aacagcttct catgtttgat gaatattgtg acttgtgacc
    18601 agttggtagg agaacaccgt ttagtggaga ggtggtgaaa gaaacgttca gccttgagcg
    18661 atgacaaaag ctctcacaaa cattgcgacg agtaaccgta ggagcaataa gaggcggtaa
    18721 aaacccgtct cttatttttc gttttctttc actgtttccc gttcaactag tacaggttca
    18781 tagagaatat ttggctgtgc ctgtcctttt tcaatcgttg tgacgatcca ccgtgcggca
    18841 tcttgtccca acttttcttt tggatgcgtc aatgttgtca attttttatt ccctaaatgg
    18901 cttaaagaag aatcgtctgt tcctacgatc gataggtcat ctggaatatg atagccattt
    18961 tctgtcaact tatctagcat taaactagct atttgatcgt tatagcaaat gatgccatct
    19021 aaagcttgtt cttctttcaa ccgttgtact gttttttcag cgattgtttc tcgtgtttcc
    19081 gtggtgaaag taatgatatc ttccgtcaaa aaacgcacat tatactgttc acacgcgctg
    19141 ataaaacctt tcatcctgta ttttccttga agatcgtcga tttttgtgat gaacaacaga
    19201 cgctgatgtc ctttttttat caaatattct gctgccataa aaccgacttt cacatcatct
    19261 aaacaaatat aagaggcatt taactcttca taagcagcat tgatcatcac catcggaatg
    19321 ccttgttctc tcaaagcaac ataaagtgct aagtttgggt tatactgatt acttttcgta
    19381 ggctcaacga tcaaaccatc taccccataa gcaatcattt tcttcaaaca ctctttctcc
    19441 aattcgtgat cgttattcgt actggctaaa agcaatgaat acccagattc tcgtaattca
    19501 ttttcgattc ctcggataat cgacggaaaa atataatcag agagatatgt ggtgatgaca
    19561 ccgatcgttt ttgttctgtt caatggtgtt ttttctttct tatagctgtc atctacatat
    19621 gtgcccgacc ctttttcttt acgcagatag ccctcattta ccaacacagc gattgcttgc
    19681 cgaatcgtat gtctgctgac gtcatatatt tcttgcagct tgaattctga gggaatggtt
    19741 gtatttactt cgtattcctc acgaaggatc ttttgtttta tctcttctgc aatatcttga
    19801 tattttgcca tttgccttgc ccctttttat ttgtacgtac atatatcata acgagtctgc
    19861 aaggtttatt caagcaaaaa tcaaggtttc ttgtgctcat aaggcatacg gaacaggttc
    19921 accaaattct ggaaatcctt gctcattcca cgtaaagcat tgcgcgttcg cgtgccgatt
    19981 aggattgtct aacggatccc ctttttggtt cttctctggt cgagcatgat agatcaaaat
    20041 atcaaagcac ccgtcttctg aaaccgtaaa cgagttgtga ccaggaccaa attgcttatt
    20101 tttttcagaa gaaacgaata ctggctccga taatttatgc catgattctt ttaataaaag
    20161 attcttgtgc tcatcagccc acagcaggcc catggcatag ttttcatctg ttgcactacc
    20221 agaatacgta atgaatattt tcccattgcg agtaaggact gctggacctt catttactga
    20281 aaaaccccgt ttttcccatt catactcagg tatagaaagg aggatctgct tcccctttaa
    20341 cgtccaagga ttatccattt cggatagata aagattagaa ttcccagtaa tttcaggatc
    20401 ttgttgggcc cacacataat atagctttcc ttgagaagag aaagttgttc cgtctaaact
    20461 gaaagtttcc cttttcgttt ttatttgtcc cttttctatc caatctgtat tcattggatc
    20521 tatatgctga ttttcaagta cgaacatgcg gtgatgatga gattcatcac gtatagtttt
    20581 atgattgctt gcagcaaagt aaatatacca ttttccattg atataatgga cttcaggtgc
    20641 ccagatcaat tcactcatcg gtccttcttc atgagcctgc catacaatca ggcttgcagc
    20701 atgttctaat tcattcagtg actttgccct tcttaattca atcacttggt agcctggaac
    20761 cgatcctgta aaataataat aaccatctgt atgtttatat acccaaggat ctgcccgttg
    20821 ccgaacgata ggattattat aaagtgtctt catcataaca aatttcctca cattttcttt
    20881 attctaatta cattccaaga gtatttttct atccctacat ttaattcgtc atgtttttct
    20941 attgaaaaat agtttatttc aggttttatt ttttctggtt cttcacgtgt atttttgtcc
    21001 aacagatcgc tactggttaa tgtgtagtgc tctccttgat ctagcaaatt gaaatcatgt
    21061 tcaatcgtta tcttttgttg ttcatttgtg cgattgatca agaaaatagt cagctgattg
    21121 ccgttatcta cgatcacact gtccacataa ggaacatttt ttatcttttc acaatcataa
    21181 acagggcaaa tgatttgttg tttcaatact tgacctttag catatttcga taagtgttgc
    21241 atcacataat aagttggatt cctccatgct tgtcctttag catctgtcag tattaaaggt
    21301 attgtgttta ctaacaatga ttgacaacta atctttacgc gatctgcgtg tcgtaaaata
    21361 gccagcccta atgaaccaaa taataatgta tcttccatag taaagtaaca ccaatcgatg
    21421 ggactaccga cttcaaaatc cctatgtctt ttttccggtt gggcatgtac attccattca
    21481 tcaaacgcta gatacattgt tttctcactt ctcaaatatc cttttacata atcacaagta
    21541 gcaacgattt cttcaatttg acgatcgaat cgataagctt ttgcaaggta tgtcgctgta
    21601 tcatcacgct catttttagg aggtttagtc aagtcttctt catcatactt atcaatatag
    21661 ttatgcaaag ctaaataatc tacattctca taagcttctt ctaatgcgat acgatcccat
    21721 tcagggtacg atggtaaacg tggcgtagag cttccaacta agattgtttc aattgaatca
    21781 tccactaact tcatgacttt tgaggcttca tttgctaacc taccatattc ataagcactc
    21841 ttagccccca cttgccatgg accgtctagc tcattcccta aacaccataa tttaacgcca
    21901 aaaggctttt tttgcccatt ttttattctt aaatcactcc agaaggttcc ttttggaaaa
    21961 ttacaatatt ctactaaatt ttttgcttca tcaattcctt ttgttcctaa attcaccgtg
    22021 aatatcggtt tcgcttgtac atgactgatc catttaaaaa attcgtggag tccaaaagaa
    22081 tttggttcta tacctcgcca tgctaaatca attttttttg gtcgttgttc tttcctacct
    22141 acagtatcca cccaatcata accagatgta aagtttcctc caggatagcg aatgacacct
    22201 aattctaatt cctttacata agagaggaca tcttgtctaa acccgtcacc atcagctgtt
    22261 tcatggcccg gttcataaat ccctgtatag acgacacttc ccatatgctc aacaaatgag
    22321 ccaaacagcc gagaactgat ctctcctacc gtaaaatcct ctctgatttt tactttcact
    22381 tcttcgtcca acagctttcc ctacctttca tttcctatag tcaagataat agatacaagt
    22441 ttccatacgc caccagcaaa gataaaaata aaaggaggga aaagaaatat gagcagtaca
    22501 aatcctaata acaatacgca acaaagaata tttctcaaaa aatcaaaaaa tactttccat
    22561 gcactttgtt caaaaatttg taaaaatgtg atatctttgt tttcagaaat cacatatatc
    22621 caggataaaa gaaacatgga ataaataagt aaaactacat atccactaat tgaaagtacg
    22681 attgtctgtt cttctaataa ttgattaaca agaaaataaa taatgatcgt taacagacta
    22741 aggaacaagt tcttaccaaa atgttgcttt attccttgaa agaaaaaata aatgatattt
    22801 cctgatccct cacctttcca ttgcgccatt acataaaaaa cagcacatgt agcaggcacg
    22861 atagtaataa caggcaaaca cacgattacc cagagaatac ctaataagat gtaatcagaa
    22921 accacaatca actgtccata aagtttagtc tcaactattt tcatcttcct cactctttca
    22981 ttccagacat agctgttcct tgaacaaaat atttctgtgc aaaaatataa accactagta
    23041 atggcagcat tgaaataacc gttccagcca ttacaggtcc ataaaaagaa atgttctgcc
    23101 ctgtaaataa agctaaccct gctggtaaag ttctcatttt tgaactactt gtcaatatca
    23161 atggataaag caagtcattc caactattca tcagcgtgaa aatccctaat gataaaagag
    23221 taggtttgct caatggcaac atgattttca aaaaaatatt aaactcactt aatccatcaa
    23281 tgcgtgctgc ctcttccaaa gaatctggta aagtaataaa aaaggaacgc atcatgaaga
    23341 taccaaatgc agttgtcata cgaggaatga tcaaacctgc ataagtatca agcatgccaa
    23401 gcaaattgac ttcaataaat agaggaatca tgaagacttg gaatgggatc atcattgtca
    23461 gcaaaacgaa atagaacaaa actgattttc ctttgaaacg catacgagca aatgcatacc
    23521 ccgcaagaga atcaaagaat acagaagtga tcactgtccc tccagcaaaa atcactgtat
    23581 ttttgatgta atctaataaa gggatcgttg tccaaacctt aatatagtta tctagggtat
    23641 agctatctgc aaatattcta ggcggaaatg agataatgtc tttttcgtgt ttaaaagaag
    23701 aaataatcag ccacaaaaaa ggaaacgcaa taaccaacaa tataactagc atactaatcg
    23761 ttcgaatgat ttttttacca ctaactgctt ttcgactatt cacagtaact gtaatcgagc
    23821 tttctggtgc catacaggtt tccctcctat ttcatttgtt tttctttccg tgacatgtac
    23881 acattagtgg agatagctat agttgcaaca atgataaaca aaaccgaaga cagtgctgat
    23941 gcatagccca attcataagg tgctgtaaat cctcgactgt aaatatattg aacagctgtt
    24001 tctgtcttaa attgtgggcc accagcggtt gcaacatata cttggtcaaa cacttgcatc
    24061 gaaccaatca gattcgttaa aatacaaaaa cctaaggagg gaacgatctg aggtagcgta
    24121 atgttaaaaa actgctgtcg atttgttgca ccatccattt gagcagattc atacaaagag
    24181 ggagagattc cctgaatagc agtcaagaga atcgtcatag taacaccaaa attcttccaa
    24241 acagtcatga tggctaccgt cggcatagca agggttggat cgcgaaaaaa ttgagggatc
    24301 ggcaagccga cttgtgaaaa caagtaagga atcgccccta cattcggatc aagcaacata
    24361 gaaaaaataa tcgcaattga cgtcaatgaa caaataactg gaatgtaaaa tgtcgaacga
    24421 caaaaacgat tgaatctggt atttttagat aatgctactg ctgctaataa accaacgata
    24481 atttgtgttg gcgtttcgag tacggtgaaa tataaagaat gaatcaacga attaactgct
    24541 cgttcatcac taaatatacg tgtgaaattt tcaaaactta caaaatttct tgatgtaaag
    24601 aaaatattca aatctgtaaa actaatagca aatgttccta aaagcggtat gacagtaaat
    24661 aataataaaa ctaaaaaaga tggagttaaa aacaaatacg cagcacgatc aggacgattc
    24721 caatatttgg tgaatccgtt catatagtcc acctcatctt tcattttgat aaaatataca
    24781 atcttggttt aaacgaatct gaataagata tccgaacaat cgctgtgatt tttgcaataa
    24841 cacctatctg caaatactag gtcgatcatt cagatatctg tatcttgcca acagtctatc
    24901 ctgtataaag ttctgtacta tttttgcagt aattgattga tggcatcatc tgcatcgttc
    24961 atgagtgtct caggatcttc tcctgctaat aatttttcaa tcagtggatt gatgatatcg
    25021 ttattgatcg cagagatttg ggtaaaacct ggtaaatatg gctctgcata gtccatttgt
    25081 ttcgataatt cagatacaat aggatcattt tttacttctt catcttttgc tactgaatcc
    25141 aagtaggcag gaaaaccatt ttcgatactc cattttttac caatttcagt tgtattccaa
    25201 tatttcaaaa actcatagac agcttctttt ttattattat ctgtacttgc cggaatggca
    25261 tatcctacgc catctaaaat agctgttttt ttgtctgaat cactcccttg tggtaaggta
    25321 gtcacattat aatcaatctc gtttgctttc aatccgttat ttaaccatgg cccattgatt
    25381 tcaatggcta gttgaccagc attcattaaa ttgtccattt cggcgccaga aatcgattct
    25441 ggaccagtct tgtctacatg gatcattttt tggatcgctt ttaacgtttt aaggttctca
    25501 ggagacgcaa attcagactt agtataatca tcgttcacta atttaccgcc atttgctaac
    25561 atccagttat ataacggtgc agttccatcc ttattgaaga caaaacctga tacattttta
    25621 ctactatcag ttaattttgg tgccatttct gtcaattctt cccaagtagt aggtggttgt
    25681 tcagtatcta atccagcttg tttaaacaaa tcattattcc aatacatata catgccttgt
    25741 acctgcatgg ggacaaaata ctgtttacct tctacttcac ctaattcaat agccgtttct
    25801 ttaaaatcag atttatctac tccatcatat tcccagaaat cgtctaatgg ttgtaccgct
    25861 ccgttttcta catactgtgc aaaatctgga taatttaatg caataaagtc aggtgctttt
    25921 ttagaagaaa tcgctgtagg aagtttttca ttcaaattgg cccaagtcat gatatccatt
    25981 tcaattttga tgtttttatc atttgtttta ttaaattcat taacgatttc ttgcaatatt
    26041 tcaccatcag atgcagtaaa cccattccaa aaagtcaaag tcacttcttt atcagaatca
    26101 gattctgctg aacttccgcc accgcaagct cctaaaatac ctgttgcagc taccaacaaa
    26161 gagccaatta accatttttt acctttcatt ttttgttcct ccttcatctg ataaacgaca
    26221 tctgaccaca ttccaagata gcggtttcaa taaacaattg attttcccat cttcagcttt
    26281 tacatttgtt gaatgacttg gttttacttg ttcactttta ggaagattag ttgcttttgt
    26341 atcaaatcca ctcatttcag aaaaatcaat cactccctct aatgataatt cagacaagtg
    26401 aacagttaga ttcatttctt cttctccacg gttaactgca aatatgacaa gttctttgtc
    26461 atttattacg gctatacttt ctaaatacgg cacttcttca aaatcttccg tcgcatagtt
    26521 tgctgaacga atttgtgggc ttaacacctc tccttgtcca tacacagcag cttgcataaa
    26581 tggataatag atcgtctgtc tccaagcttc tccgtttgtt tctgtcctga ttggtgcaat
    26641 aacatttacc aattgagcaa gacaagcaat ctttactcta tctgcatgtt tcaataaagt
    26701 gattaataga cagcctaata gtaatgcatc ttcaaaatta tagttatctt ctagtaaagg
    26761 aggagcaatc tgccaaggcg ctgcttgctc atcttgttcc gctgaatgat accaaatatt
    26821 ccattcatca aaggacaggt tgatctgctt cttactatgt ttcttcgcct tgactgtatc
    26881 gcacatcgct gtcacttcag aaatgaaccg atccatgtct aaggaacgtg caagataatg
    26941 agaaagatca tttttttggt tgccataata ttgatgcaga gacaaataat ctatttgatc
    27001 gtacgcttga tcaagaactg ttaactccca atcaccaaat gtaggcattt gccgattcga
    27061 acttccgcat aaaaccacct caattgaatc atctacgagt ttcatcgcct tagccgtttc
    27121 ctctgctaac cgtccatatt cttccgctgt tttatgtcca atctgccaag gaccgtccat
    27181 ttcattccct aaacaccatg tcttaattcc aaaagggctc tcttgaccat tcgaacgcct
    27241 taaatcactc caataagttc ctttaggaaa attacaatat tcgactaaat tacgagctgc
    27301 atctactcca cgagtaccca gattgacagc catattgatt tcggccccca catgctgaca
    27361 ccagttataa aattcatgaa tcccaacttc attcgtttca attgtccgcc aagctaaatc
    27421 caaccttcta ggtctatttg ctatgggccc aatactgtct tcccaacgaa atcctgacac
    27481 aaaatttcca ccaggatatc taatcatggg aatgttcaat tcttttacca agtgaatgac
    27541 gtcttggcga aatccacttt tattagctga tggatgatct ggctgataaa tcccttcata
    27601 tactgctcgt cccatatgtt caataaatga actgtacaac cgcggatcaa ctttgcctac
    27661 acgcttgaca ctactaatca acattgtggc ttccataact gacctccttt aatttataca
    27721 tgaataatac acctcgttga aagcgttgtc aataatataa atgttaaact tgcttatttt
    27781 ttatcgattt taattaacca aaatattaat aatgattaaa acaaataatt taatataact
    27841 tttattaaca aatatgttaa tatataagaa aaacctttta agaggtgata tatatgcaac
    27901 tacaaataaa cgaagaatcc ttaccggtat atgaagcatt agctagtaaa acaaggatca
    27961 agatcatcca gctactttcc aaaaaaaaga tgaatgtcaa agatttggca aaagaacttg
    28021 gagtgagcag tgctatcacg acgatgcacg tgaaaaagct agaggaagca aacatcatca
    28081 aaacagaaaa agtaggacaa caaaaaattt cgagccttcg tgtcgataaa atcgatatca
    28141 gttttccaga aaaaatattc aatgcttttg atacgaaaga aacatcgata cctattggtc
    28201 actatacaaa ttatgcaatt gaaccaacat gcggtttagc tacgatccat gattttatcg
    28261 ggaaagtgga tgaaccacgc tattttatgg atcctcgaag gatggatgcc cgcattctct
    28321 ggtttaccag cggatttgtc gagtatcagg cacctaattt tttaaataca gaggatacat
    28381 tggagatgtt ggaagtttct gtggaaattt cttctgaatt tcctttttca aatgacaatt
    28441 ggccttcaga tatcactttc agcctaaatg gtgtggagct aggaacatgg acgagccctg
    28501 gagattttgc tgatatccgc ggtaaatata cgcctgattg gtatccagat aatcttaatc
    28561 aatacggctt gctgaagacg atccgaatta ccaaacactt gacgaatatg aatggagaac
    28621 ccttgtcaaa catcacgatc aacgacatcc ctaaagagca agacacttgg catttgcgaa
    28681 tcgaagtgaa agacgatgcc aaacatgtgg ggggctgtac tttatttggt aaaggattcg
    28741 gtaattacga tcaggatatc aaattaaaag tttactacag ctgatatttt tcttttctat
    28801 tttataaaac aaaaacagcc aatccctatg aagggacggc tgtttttgct ttattgttat
    28861 ttccagaaat catccaaaac ggaaattggc aaatgtcgtt tatgttgtgt cttgttccac
    28921 catgcttcga ttttttcttg tgcttgagcg ggaacttctt ttccttctag ataatcatca
    28981 atttcattat atgtgacacc tagtgcgact tcatctgcaa tcattggttt cccatcttca
    29041 agatctgcag tcggaatttt tgtgtataat ttttccggtg cattcaatgc ttgaagcaat
    29101 tgtttccctt gtcgtttgtc caaacggaat aatggcaaaa tgtcggctcc accatcacca
    29161 aatttcgtga agaatccagt aatgttttct gcagcatgat ctgtccctaa tacggctcct
    29221 gctctttctc cagctacggc atattgcgta atcatacgtt gacgtgcttt gatatttcct
    29281 ttgttaaaat cgctgattcc cacacctgct tcgcttaata cttgtacttg tgcatcaaca
    29341 gctggtttga tatttactcg cagactaaca tcgggctgga taaagtcaag agcagctttt
    29401 gcatcttctt catctgcttg ttcaccataa ggaagacgaa cagcgataaa ttgatagttt
    29461 tcatcttttg tttcttcacg catttcttcc atcgttagtt gcgccaaacg gcctgctaaa
    29521 gtagaatctt ggccgccgct gatacctagt acaaaagttt taagaaacgg atattttttc
    29581 aaatatgctt tcataaagtc aatactttta cgtatttctt cttttggatc aattgttggt
    29641 ttgacaccta attcattgat aatttctgtc tgcaggctca tttattctcc tcctatgctt
    29701 ctgtatgaag ttctgctacg ctttttttca ttcgttccat gatagccatt ttatggttcc
    29761 agcattcagt agacagatcg actgggtatt tttgcggatt caagtctcgt ttatactctt
    29821 cccatagtga atctagattt tcttttgtat attctttgat ttcgtttagt gcaggtaatt
    29881 cgtaaactct ttttccttcg ataaagatat cttgtaaaac tgggcgagct gtaaagtctc
    29941 gaacagtttt attgataaac gtatgcactg gatggaacat gaagatagct tcttcttctc
    30001 gagggtcttc atcccataat gtcacgtagt ctccttcaga tttcccatca aaatttcgag
    30061 tgatgcgcca cacttgtttt ttccctggag tagttacttt ttctgcattg cttgataact
    30121 tgatcgtatc catcattttt ccttcatcat cttcgatcga tactagcttg aatactgcac
    30181 caagggctgg ctgatcatag gctgtgatca attttgtccc aacaccccat acatcaattt
    30241 tagctttttg catctttaaa ttcaaaatcg ttgcttcatc caagtcattc gaagcataga
    30301 tttttgcttc cgtaaatccg gctgcatcta attgttcccg aacacgctta gatatatagg
    30361 ccatatctcc actgtcgatg cgtacgcctt ggaagttgat tttgtcacct aattctttcg
    30421 ccacgcgaat cgcacttggc acacctgatt tcaacgtatc ataagtgtcg actaagaaca
    30481 cgcagtcttt atgggtttta gcgtaggcca taaatgcttc ataatcattt ccataagatt
    30541 ggactaacga atgagcatgg gtcccgctag ctggtatccc aaatatcttc ccagcacgta
    30601 cattgcttgt tgcatctgct ccgccgatat atgccgctct agttccccag actgctgcat
    30661 ccagttcttg tgcccgtcta gttccaaatt ccaatagtgg atcttctccg atgacagatt
    30721 tgatacgagc tgcttttgtc gcgatcaacg tttggaaatt gaccatattc aacagtgctg
    30781 tttctactaa ttggcagtgc gctaaaggcc cttccacttg gattaatggt tcatttgcaa
    30841 aaactagttc tccttcacga gcagaacgaa ctgttgcttt aaattcaaaa ttttgtaaat
    30901 actccaaaaa accttcagga tagacttcta atccacgcaa gtaagcaata tcggaatcat
    30961 tgaacgtcag attctctaaa taattgacta accgttccaa tcctgcaaaa accgcgtatc
    31021 cgtggttaaa aggcatttct cgaaagtaac attcaaaaac agcatgtaaa tcagctcgac
    31081 ctaattccca gtatgtctgc atcatattga tttgatatag atccgtatgc agcgtcaaac
    31141 tgtcgtctgg gtaagtatta ttcatcttgt attactcctc ttggcaagtt ttcatttcct
    31201 acccattata acaaaaaaaa ttaataggaa aagaaatctt ccattttttc tgaaaaagca
    31261 catgttttac ggatatttct tgctaaaaat gaactccatc tgctaaaact atcctttctt
    31321 attggtattt aacctaaacg agaaagaata cttattttat tcaatgtatc cgagacacga
    31381 agaaattagc gttttctatt tttcttactt gataaaataa gtagattttc ctactacaat
    31441 cgtagccgtt aagataccga tgattgaaca aatataacca aatgtctcta caacataatc
    31501 aatggcaaaa gtttttgaag caataatacg tctttccttt tttgcttctt atccccaaag
    31561 aaacggatat aacttattat gtattgaatt tttttggaaa ggcgaattat gacaagatat
    31621 accttgcgtt gaaaaaactc gcggaaaaaa tatcacacgt ttttaaaaag atacaaagta
    31681 agaaagcagc tgtcaataga atggaaacaa aaatggcgag acatcaatca gtctcgcctt
    31741 gttttttatt gtatacatta gatatggagc aggttaccat aaagatgatt attagtcgtt
    31801 attaaataga atccttcgta tttttttgtt ctatcactac aatatcttta tttggacgtt
    31861 tgaattgtat tccggcacgt tctagctcct gtttcactcc tacatggact ttgacatctt
    31921 ctcgtgtcat ttttaaaacg tgatgaatcg catagtcgat ttcttgcctt gtgatttttt
    31981 tcttcgtatt gatcatcacg acttgctttt tatcatatgt gtctaccaat agcagttgtg
    32041 ttcgatctaa cgaaaataat ttgtaataat taatagccgt attcccaaaa aagaattcca
    32101 ctactttagg ataaaatgtc ttaatattta aattactatt cacaggtgtt tcgattagct
    32161 tcatctcatt cctcttttct atatagtcga tttagatcca atcttaagtt atgttaccat
    32221 ctttcctagc attaaggaat cggttacaat tgaaccttta aagacaaaag gatttacctt
    32281 ttatcactta atagtataac ataatttcgt gagaaaaaaa tacaattttt tcttgaccat
    32341 ctcgttacgt aaaggattat gcttacattt gtcaggagga aaaataatgg agtacacaat
    32401 aaagaaaatg tcagagatat caggagtcag tccgagaacc ttgcgctttt atgatgagat
    32461 tggactgcta aaaccagcta ggatcaattc ttcaggttat cggatttatg gcaaaaaaga
    32521 agtcgatcgt ctgcaacaca tcttatttta tcgaacaatg gcattcaagt tggatgatat
    32581 ccaagaggtc ttagacaatc catcttttga ccaccagaaa gctttaatca agcatcagca
    32641 aatgttgctg gaaaaacggg cacaaatcga taccctcttg acaactgtcc aacagacact
    32701 tgatatgtac gaaggaggaa gaaaaatgag tgacaaagaa aaatttgaag ggtttaaaca
    32761 acaaaagttg aaagaaaatg aagaatccta tggacaagag attcgacaaa aatacggaaa
    32821 aaaaaaggtt acagcatcca atgaaaaatg gatgaatatg gataaagaaa cgtatgaaga
    32881 aatgcaaact gttgaaaagc agatgttatt tgatctcact ctttacttaa aagaaccaga
    32941 agatgaaagc ttagcagacc gtatcttcca agctcataag aagtggttct cttacagctg
    33001 gcctgaatat cagccaaaag cgcataaagg actaggaatg atgtacgaag ccgatgaacg
    33061 gttcactgca tattatgacg agcgaagtgg tcatggtgca gcaaaagctt taaatgaaat
    33121 tatccaaaaa aaggcgtaat cttttacatg atccaacaaa aaagtacacg taaaatgacc
    33181 taataacaaa acagcagaca cccaagttag atttttatgc taacttttgg tgtctgctcc
    33241 attctttcac aagttttttc atcctacttt aaatatattc atcgttgaat aaacgtataa
    33301 ggtattttca ctttatcctt tttttctgta agaaacaatt gaaatgccat ttctccgcaa
    33361 gcttccagat ggtggtcgat ggttgggaaa ccaagaagct cgctgatcag caaattatcg
    33421 cgtccaatca ccactggtgt cttatcttgc agatagcttt gcaagatgcc tgctgcaacc
    33481 atatccgaat ttgtaaagac accatcaact ccaagctctt taaaaaagaa gcctgcttgt
    33541 tttcctttct ctgcatctat acaatcccaa aaaacaagtg attcatcaaa agatggaaaa
    33601 agttgatgac atagctgcaa agtgattttt gagttataac ttagcctgcc acttcgcccc
    33661 aacgtgatgc ccaattgctt tataccttgc tgcttcaaat aagtaagacc ttctgtaatt
    33721 gattgttctc gatcaataaa gacacagctt gcctgttttc ccttgatttc ttcacaaaaa
    33781 actagaggtc catatttttg atatgccaat aaatcatcta gtgtattggc acgagacgta
    33841 atgatcaatc catcgaaagc tttagctgca aattcttcaa gataccttcg ctctttttct
    33901 ttatgatagc ccgtcggtaa aagtgtcact ttgtaatcat aatcaaacgc tgcttcaatg
    33961 atcccactga tcaactgatc aaaatacgga tgattggtat aaggcagaat cactccaatg
    34021 cttttcgttt ttccataact taactgtctg gcatttgcat tagggatata atcgacttct
    34081 ttcatgaccg caagtatttc tttccttttt tcctcatcta catatggatg tccattgatt
    34141 acacgtgaaa tagtagaaac tgaataacct gtgagctttg cgatatctcg aatgtttgcc
    34201 actgctgctt cgccacctat acaaaaagat tttcttgtaa atagtctacc acagctggac
    34261 cgcattcaaa aaactgatac atttttcctt catgcgaaaa actgatccgc tgcccgcccc
    34321 aatatatatc ttttgtcaaa ttttcgattt cattcattgg tatttctttt actatattcc
    34381 tattcaaccc ttgaaaactg tacaacgtta atacggatcc cttgaactcg acaaacgcat
    34441 aaaaataatt gttcgttgtg ccatctgtta tctctacata agtattcatt gcaagccctc
    34501 cttttgatac tttgagtata gtgtatgaaa cgcgtctcat agcaaggagt ttgttttgcc
    34561 aaagaaaagt tttattcctc tttcattatt accaaagtct cttattttaa attgaataca
    34621 ttataatgat actgtaaata tatctttatt attagtgaat g
//
LOCUS       DACGLE010000023        34427 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0534
            SAMEA3304449-rid9560453.denovo.024, whole genome shotgun sequence.
ACCESSION   DACGLE010000023 DACGLE010000000
VERSION     DACGLE010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304449
            Sequence Read Archive: ERR985064
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34427)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34427)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702225.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702225.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 20:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,045
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 27 of 67
            Pseudo Genes (incomplete)         :: 25 of 67
            Pseudo Genes (internal stop)      :: 19 of 67
            Pseudo Genes (multiple problems)  :: 4 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34427
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304449-rid9560453.denovo.024"
                     /strain="VREN0534"
                     /db_xref="taxon:1352"
     gene            407..1096
                     /locus_tag="IXT20_001228"
     CDS             407..1096
                     /locus_tag="IXT20_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ4859684.1"
                     /translation="MKILIADDDKEIVELLSIYVHNEGYEAVKAYDGKEALSKIRTIP
                     DIELLILDIMMPEMDGMQVVKELRKESQIPIIMLTAKTTDMDKIKGLVAGADDYVTKP
                     FNPLEVMARVKSLLRRTKMQIAPDQPDVLEVNALIINKDSHEVKTIDGKEIQLTALEF
                     GILYLLASHPNRVFSADEIFERVWKQESIVSAKTVMVHVSHLRDKIEEATGGEKVIQT
                     VWGVGYKIDAR"
     gene            1083..2291
                     /locus_tag="IXT20_001229"
     CDS             1083..2291
                     /locus_tag="IXT20_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAQ4859685.1"
                     /translation="MTQDSKRATEKRRRINLTSKEISELLAEGIITIILLLLLNVSIL
                     VVISSVINSSPSLTNAIWDSKNIFAERLNTDLFWNGRNFIIPFFFLLDIGVLYWRLIR
                     RYRQMQLRHIISELHYIANGNYDHRIPFELSGDLSRVVTSINGLVDSTVAAIEDERKI
                     EKSKDELITNVSHDIRTPLTSIIGYLGLIEDGQYHSEEDLLKYTHTAYIKAKQMKSLV
                     DDLFEYTKVRQPAVPVNFSAFDMIQLIEQLAADFELEASKKNIQILVQSKVDSLIMDG
                     DTEKLVRVFNNLLTNALKYGKGATKIVIEVERIGSEVVATVKNNGAMIPQQAIDNLFD
                     RFYRVEESRSQATGGTGLGLAIAQSIVALHGGYIYAKSDKQWTSFIMHLPIKKNEKLP
                     IDTQEVIDES"
     gene            2585..3856
                     /gene="serS"
                     /locus_tag="IXT20_001230"
     CDS             2585..3856
                     /gene="serS"
                     /locus_tag="IXT20_001230"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HAQ4859686.1"
                     /translation="MLDVKMMRQNIEEVQEKLMTRGVKKETLAEFMELDENRRKLLVK
                     SEELKKYRNDVSAEIAQLKRAKEDASAKIAEMKEVGGNIKALDTEIAEVDEKLKQIAT
                     TLPNLPHDSVPVGTDEEDNVEIRRWSEPRTFAFEPKPHWEIAENLDILDFERGAKVAG
                     SRFVYYKGLGARLERAVYNFMLDQHIYEHGYTEMITPYIVNSKAMFGTGQFPKFKEDV
                     FQLENTDLTLIPTAEVPLTNYYNDEILDGADLPIYFTALSPAFRSEAGSAGRDTRGLI
                     RLHQFNKVEMVKFSDVEHSYEELEKMTADAEDILKKLHLPYRVMALSTGDMGFSAAKT
                     YDLEVWIPAQDTYREISSCSNCEDFQARRAMIRYRDNDGKIHYTHTLNGSGLAVGRTV
                     AAILENYQNEDGSVTVPEALVPYMGNLKVIK"
     gene            complement(4405..5889)
                     /gene="guaB"
                     /locus_tag="IXT20_001231"
     CDS             complement(4405..5889)
                     /gene="guaB"
                     /locus_tag="IXT20_001231"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HAQ4859687.1"
                     /translation="MSNWETKFTKKGFTFDDVLLIPAESHVLPNEVDMSVQLAKNIKL
                     NIPIISASMDTVTDSKMAIAMARQGGLGVIHKNMTISQQADEVRKVKRSESGVIIDPF
                     FLTPQHLVADAEELMSKYRISGVPIVETLENRKLVGIITNRDMRFVTDYHMPIADVMT
                     KDNLVTAPVGTSLKDAEKILQKHKIEKLPIVDNEGRLSGLITIKDIEKVIEFPNAAKD
                     EHGRLLVAAAVGVTSDTFERANALLEAGADAIIIDTAHGHSAGVIRKIQEIRSTFADA
                     TLIAGNVATAEATKALYDAGVDVVKVGIGPGSICTTRVVAGVGVPQLTAIYDAASVAR
                     QYGKAIIADGGIKYSGDIVKALAAGGHAVMLGSMLAGTDESPGEFEIFQGRRFKTYRG
                     MGSLGAMEKGSKDRYFQGSVNEANKLVPEGIEGRVAYKGSVADIIFQMIGGLRSGMGY
                     VGAANLQQLRDEAQFIQMSGSGLKESHPHDVQITKEAPNYSVES"
     gene            complement(6394..7089)
                     /locus_tag="IXT20_001232"
     CDS             complement(6394..7089)
                     /locus_tag="IXT20_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1129 domain-containing protein"
                     /protein_id="HAQ4859688.1"
                     /translation="MEPEQLRAIVKENNSLETKLTKRNQQYIFDLKKSLEAANLSEEE
                     KVKALHDILPTLVAEQKGGRTARQLFGTVSERTEAIINKPAEVKNSSTPLLMWLDNTL
                     LLLGLLSIMIAITGLFSRGTTPAYGFSTLVLGSAIGGWVFYMMYKYIYQYEYPGADRS
                     KKPGMGKTLLILGGTTLLWVVLFSATTLLPPVINPVLDPVVVIIIGATALAIRYYLKK
                     KYGIIGSLSVPRR"
     gene            complement(7179..8279)
                     /gene="ychF"
                     /locus_tag="IXT20_001233"
     CDS             complement(7179..8279)
                     /gene="ychF"
                     /locus_tag="IXT20_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HAQ4859689.1"
                     /translation="MALTAGIVGLPNVGKSTLFNAITKAGAEAANYPFATIDPNVGMV
                     EVPDWRLQRLTELVHPKKTVPTTFEFTDIAGIVKGASKGEGLGNQFLSHIRQVDAICH
                     VVRCFDDDNITHVEGRVDPLEDIDTINLELVLADLESINKRYTRVAKVAKTKDKDAVA
                     ELAVLDKIKPVLEEGKSARTIEFTEEEQKIVKSLFLLTTKPILYVANVSEDDVADADA
                     NHYVQEVRNFAAEENAEVIVVSARAEEEIAELDEEDKAEFLEALGIEESGLDQLIRAA
                     YDLLGLATYFTAGEQEVRAWTFKKGIKAPQAAGIIHSDFERGFIRAETVSFEDLDHYG
                     NMHAAKEAGRVRLEGKDYVVQDGDVMLFRFNV"
     gene            complement(8288..8479)
                     /locus_tag="IXT20_001234"
     CDS             complement(8288..8479)
                     /locus_tag="IXT20_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HAQ4859690.1"
                     /translation="MYDLHDIVEMKKPHACQTNRWEVIRMGADIKIRCMNCGHIVMMT
                     RRDFEKKLKKVIEKNAEKE"
     gene            complement(8500..9390)
                     /locus_tag="IXT20_001235"
     CDS             complement(8500..9390)
                     /locus_tag="IXT20_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HAQ4859691.1"
                     /translation="MKKSKGLGRGIDALFQDLENLETVDVKDDTVVQLPLNELRPNPY
                     QPRKTFEEVSLQELANSIEQSGVFQPIIVRRSAVKGYEIIAGERRFRASKLAEKETIP
                     AIVRDFDEEAMMQVAVLENLQREDLNPLEEAEAYEMLMKNLKLTQAEVASRLGKSRPY
                     IANYLRLLSLPDLVKEMVQDQRLSMGQARTLLGLKNKTLILKLANRAVTENLTVRQLE
                     QLVSELNQNDGKEKKKIPRLAKEKPYYIRESEDRLMDKFGTSVAIQEKNGKGKIEIEY
                     LSQKDLTRILDILNIQFDEE"
     gene            complement(9377..10144)
                     /locus_tag="IXT20_001236"
     CDS             complement(9377..10144)
                     /locus_tag="IXT20_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HAQ4859692.1"
                     /translation="MAQIISVANQKGGVGKTTTTVNLGACLASLGKKVLLVDMDAQGN
                     ATSGVGIRKPDVTRDIYDVLVNELPIDEATLITEHENLSIVPATLQLAGAEIELTSMM
                     ARESRLKGSLAEVSSQYDYILIDCPPSLGHLTINSFTASDSILIPVQCEYYALEGLSQ
                     LLNTVRLVQKHFNPELEIEGVLLTMYDARTNLGNEVVEEVRKYFREKVYETIIPRNIR
                     LSEAPSHGKPIIDYDPRSRGAEVYQALAKEVVSREEK"
     gene            complement(10156..10869)
                     /gene="rsmG"
                     /locus_tag="IXT20_001237"
     CDS             complement(10156..10869)
                     /gene="rsmG"
                     /locus_tag="IXT20_001237"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HAQ4859693.1"
                     /translation="MIPEEFRQSLAEKGIELTDHQMNQFAIYYRTLVEWNEKMNLTAI
                     TDEKDVYLKHFYDSLTLAFEVDFTQPLSLCDVGSGAGFPSIPLKIAFPELQITIVDSL
                     NKRITFLSELTRLLGIDASLYHDRAETFGQNPHFRASFDLVTARAVARLNVLAELCLP
                     LAKQEGYFYALKAAKSEEELTEAKQAIALLGGKFLKEEAVTLPNVSDQRHIIVIQKKK
                     PTPKKYPRKPGLPNKQPIK"
     gene            complement(11101..13002)
                     /gene="mnmG"
                     /locus_tag="IXT20_001238"
     CDS             complement(11101..13002)
                     /gene="mnmG"
                     /locus_tag="IXT20_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HAQ4859694.1"
                     /translation="MEHYEADAYDVIVVGAGHAGSEAALAAARMGSKTLLLTINLDMV
                     AFMPCNPSVGGPAKGVVVREIDALGGEMGKNIDKTYIQMRMLNTGKGPAVRALRAQAD
                     KHAYSTEMKHTIEKEPNLTLRQGIVEELIVEDGVCHGVVTSTGASYRAKAVVITAGTA
                     LRGEIIIGELKYSSGPNNSQPSIGLANHLKELGFAIERFKTGTPPRVKSSTIDYSVTE
                     EQPGDKEPNHFSFSTPDSAYKLDQESCWLTYTGETTHQIIRDNLHRAPMFTGIVEGVG
                     ARYCPSIEDKIVRFADKPRHQLFLEPEGLNTEEVYVQGLSTSLPEDVQVNMLHSIAGL
                     EKVEMMRTGYAIEYDVVEPHQLRPTLETKVVENLYTAGQTNGTSGYEEAAGQGLIAGI
                     NAALKVQGKEPLVIKRSDGYIGVMIDDLVTKGTNEPYRLLTSRAEYRLLLRHDNADLR
                     LTEMGHRVGLVKEDQYKNYLKKKEAVEQEIKRLMNVRIKPTEEVQDFLATINAAPLKD
                     GVLASDFLRRPEITYAELLRFIPENEELTRKEIEQVEIQIKYEGYIKKAMEKVEKLKR
                     MEAKKIPENIDYAAINGLATEAKQKLQKIQPETIAQASRISGVNPADLSILMVYIEQG
                     KIAKVSEPA"
     gene            complement(13019..14416)
                     /gene="mnmE"
                     /locus_tag="IXT20_001239"
     CDS             complement(13019..14416)
                     /gene="mnmE"
                     /locus_tag="IXT20_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HAQ4859695.1"
                     /translation="MAEITLEFDTIAAISTPPGEGAISIVRLSGDQAVQLADKVYQSG
                     NKRLSEVPSHTIHYGHIVDPKSNQLVDEVMVSVMRAPKTFTREDVVEINCHGGIVVVN
                     QILQLLLREGARLAEPGEFTKRAFLNGRVDLSQAEAVMDLIRAKTDKAMGLALNQLDG
                     NLSALIRSLRQEILETLAQVEVNIDYPEYDDVEELTTKLLLEKAQMIQQRIQALLATS
                     KQGKVLREGLSTAIIGRPNVGKSSLLNHLLREEKAIVTDIAGTTRDVIEEYVNVRGVP
                     LKLIDTAGIRETEDVVERIGVERSRKALAEADLILLVLNQSEPLTAEDEQLLEATSGL
                     KRIILLNKTDLPAQLEQEKLKKLIENEPVFSISVAKNDGLDRLESAISDLFFSGETGE
                     RDATYVSNTRHIALLEKASLSLEEVIAGIDSGMPVDLVQIDMTRCWDYLGEVVGDSVQ
                     DELITQLFSQFCLGK"
     gene            14760..15098
                     /locus_tag="IXT20_001240"
     CDS             14760..15098
                     /locus_tag="IXT20_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4859696.1"
                     /translation="MSPFRSAALEEESDPRINDVVLSTGNINQKYIVRDIVFVVECLE
                     ADLFDPSFPKDDLFLTTKRKLKTKVLEYGANAVINCHFEYQQKKRDGKYFLELFAYGT
                     VIQFIQTTIG"
     gene            complement(15266..16024)
                     /locus_tag="IXT20_001241"
     CDS             complement(15266..16024)
                     /locus_tag="IXT20_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HAQ4859697.1"
                     /translation="MPIYEAATIDEAVQLGLADLGLAKDQVEIEILEEGKKGFLGMGR
                     KNARLSIQPSISEKVSETVEQTVEEILEEEPTAEIEVVNETILEEKTEEVSELGDEAA
                     LTELAVYLTEISKQLDAPALVKMERESNNMVVFHLETQKQGILIGKHGKTLNALQYLA
                     QVFIHRLARNKLSVVVNVGNYREKRQEIIQRLADRTAEKVKRTGQPVFLEPMPAFERK
                     QIHAALSKDEHVKTHSEGEEPYRYLVVEPAKKYY"
     gene            complement(16042..16860)
                     /locus_tag="IXT20_001242"
     CDS             complement(16042..16860)
                     /locus_tag="IXT20_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HAQ4859698.1"
                     /translation="MKKYKRLLLMAGIVSLVIVLSGCGTGEVSAQSTGIWDRYIVYYF
                     AEAIKALSFGNAGIGIILFTLIIRVILLPLMHFQTKSMRKTQELQPKLKALQQQYSSR
                     DPETQSKLREEQQRLYAENGVNPYAGCLPLLVQMPILMALWQSISRVPELKQGHFLWL
                     NLGNADPTFILPILAAIFTFASTYLSSMSQIESNASMKIMNFVMPVMILVMGVNLASG
                     LSLYWVVSNAFQVVQTLLINNPFKIRKEREEEARRIRERERALKKAMNPKKKRK"
     gene            complement(16857..17213)
                     /gene="rnpA"
                     /locus_tag="IXT20_001243"
     CDS             complement(16857..17213)
                     /gene="rnpA"
                     /locus_tag="IXT20_001243"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HAQ4859699.1"
                     /translation="MRKAYRVKKEREFQAVFHTGDSFANRKFVVYRLEKPGQPHFRVG
                     LSVGKKVGNAVNRNEVKRKIRAAVYSIKEEIDPELDFIVIARPSVKGLTYDEVRSNLV
                     HVLKLAKIIDREEKSK"
     gene            complement(17331..17465)
                     /gene="rpmH"
                     /locus_tag="IXT20_001244"
     CDS             complement(17331..17465)
                     /gene="rpmH"
                     /locus_tag="IXT20_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003397591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HAQ4859700.1"
                     /translation="MKRTYQPNKRKRQKVHGFRKRMSTKNGRRVLASRRRKGRKVLSA
                     "
     gene            17988..19322
                     /gene="dnaA"
                     /locus_tag="IXT20_001245"
     CDS             17988..19322
                     /gene="dnaA"
                     /locus_tag="IXT20_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HAQ4859701.1"
                     /translation="MVSLDALWNELKATYQKDLSPASYNTWIETANPRTLDQNQLVVE
                     VPSKIHKEYWEKNLATKIVEVGYMLSGNEIIPRFITGEEAEQEEVIEEKNPKVVAPSP
                     LKKAMLNPKYTFDTFVIGKGNQMAHAAALVVAEDPGSIYNPLFFYGGVGLGKTHLMHA
                     IGHQMLQSQPNAKVKYVSSETFANDFINSIQNKTAEEFRQEYRNVDLLLVDDIQFFAE
                     KEATQEEFFHTFNALYNEGKQIVLTSDRLPNEIPKLQERLVSRFAWGLSVDITPPDLE
                     TRTAILRKKAAAERLEIPDDTLSYIAGQIDSNIRELEGALVRVQAFAAMNSEDISTSL
                     AADALKTLKSGKGHPQLSILQIQEEVAKYYHIQLKDLKGKKRVKSIVVPRQIAMYLAR
                     ELTDNSLPKIGAEFGGKDHTTVIHAHEKIQQLMDSSVSMQNEVSEIKNFLLN"
     gene            19520..20650
                     /gene="dnaN"
                     /locus_tag="IXT20_001246"
     CDS             19520..20650
                     /gene="dnaN"
                     /locus_tag="IXT20_001246"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HAQ4859702.1"
                     /translation="MKVTLNRASFMQELQTVQRAISSKTTIPILTGVKITLTQEGLTL
                     TGSNADISIETFLSVENEKANMQIESTGSIVLQARFFSEIIRRLPEETFTLEVLENKQ
                     VAITSGKANFIVNGLDADNYPHLPVVESHNQMKLPVHVLTKLINETVFAVSQHESRPI
                     LTGVHFILSDNSLLAVATDSHRLSQRVIPVEQAADHFDIVIPGKSLIELSRSLTNEEE
                     IVEISIMENQVLFKTETMYFYSRLLEGNYPDTNRLIPSSFNTEVEFSVPSFLAAIERA
                     SLLSHEGRNNIVRLSIRPDAVVLYGNSPEIGKVEESLSYTASSGDPLDISFNPDYMKA
                     ALRAFGDMSIKVKFISAIRPFTLEPTEDGVQFIQLITPVRTN"
     gene            20879..21121
                     /gene="yaaA"
                     /locus_tag="IXT20_001247"
     CDS             20879..21121
                     /gene="yaaA"
                     /locus_tag="IXT20_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HAQ4859703.1"
                     /translation="MKQQVILNTEFMTLGQMLKEVTVIGSGGQAKWYLAENTVLVDGE
                     PENRRGRKLYPGMMVEVPEVGTFFMVKKGNDDNEAE"
     gene            21108..22232
                     /gene="recF"
                     /locus_tag="IXT20_001248"
     CDS             21108..22232
                     /gene="recF"
                     /locus_tag="IXT20_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HAQ4859704.1"
                     /translation="MRLNKLYLKNYRNYEELNIEFSKNLVIFLGENAQGKTNLLESIY
                     VLAMTRSHRTSSEQELIGWTDDQAMIQGEITKGSSTLPLEILLSKKGRKTKINHIEQK
                     KLSTYVGQMNVILFAPEDLSLVKGSPQTRRKFLDMELGQINPIYLYDLVQYQAILKQR
                     NQYLKQLNEKKQTDLLYLDILSEQLAAFGSKVLKARAQFVKRLEFWANSLHQQITHQK
                     EQLEIEYLTAVDSLETHTQEQIQEQFLALLNQNKKKDLFRGTTTVGPHRDDLSFFINQ
                     KNVQTYGSQGQQRTTALSVKLAEIDLIKEETGEYPILLLDDVMSELDDNRQLHLLETI
                     EGKVQTFLTTTTLDHVKNKMTVEPEIFYVQQGKLKGEETE"
     gene            22229..24175
                     /gene="gyrB"
                     /locus_tag="IXT20_001249"
     CDS             22229..24175
                     /gene="gyrB"
                     /locus_tag="IXT20_001249"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003129249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HAQ4859705.1"
                     /translation="MTEERSLVERAKEYDASQIQVLEGLEAVRKRPGMYIGSTSSEGL
                     HHLVWEIVDNSIDEVLAGFATKIHVIIEKDNSITVIDDGRGIPVDIQAKTGRPAVETV
                     FTVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSTQLDVKVYKEGNVYYQEYRRGAVVD
                     DLKIIEQTDRHGTTVHFTPDPEIFTETTEFDFSKLATRIRELAFLNRGMRISIEDKRE
                     EEPVINEYHYDGGIKSYVEYLNANKTVIFPEPVYLEGEQQDIAVEVSMQYTDGYHSNI
                     MSFANNIHTYEGGTHESGFKTALTRVINDYARKQKLMKENDDNLTGEDVREGLTAVIS
                     IKHPDPQFEGQTKTKLGNSEVRTVTDRLFSEHFMKFLLENPSVGRQIVEKGLLASRAR
                     LAAKRAREVTRRKGALEISNLPGKLADCSSNDPEKCELFIVEGDSAGGSAKQGRNREF
                     QAILPIRGKILNVEKASMDKILANEEIRSLFTAMGTGFGEDFDVSKARYHKLVIMTDA
                     DVDGAHIRTLLLTLFYRYMRPIVEAGYVYIAQPPLYGVKQGKNITYVQPGKNAEEELK
                     QVVASLPASPKPSVQRYKGLGEMDDHQLWETTMDPSNRMMARVSVDDAIAADQIFEML
                     MGDRVEPRRAFIEENAHYVKNLDI"
     gene            24195..26666
                     /gene="gyrA"
                     /locus_tag="IXT20_001250"
     CDS             24195..26666
                     /gene="gyrA"
                     /locus_tag="IXT20_001250"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HAQ4859706.1"
                     /translation="MSEEIRENIHDVNLTSEMKDSFIDYAMSVIVARALPDVRDGLKP
                     VHRRILYGMNELGVTPDKAHKKSARIVGDVMGKYHPHGDYAIYESMVRMAQPFSYRYM
                     LVDGHGNFGSVDGDGAAAMRYTEARMSKIATEMLRDINKNTVDFQSNYDDTEKEPVVL
                     PARFPNLLVNGTTGIAVGMATNIPPHNLSEVVAAIDLLMDNPDVTTNELMEVLPGPDF
                     PTGGLVMGKSGIRRAYETGKGSITVRAKVEITEMPNGKERIIVTELPYMVNKAKLIER
                     ISELHRDKRIEGITDLRDESSREGMRIVIDIRRDASASVILNNLYKLTSLQNSFGFNM
                     LAIEKGVPKVLSLKQILENYIEHQREVITRRTVFEKEKAEARAHILEGLRIALDHIDE
                     IIAIIRGSKSDDEAKATMIERFDLSDRQSQAILDMRLRRLTGLEREKIENEYQELLKL
                     IEDLTDILARPERVTEIIKNELADLNQRFGDKRRTELLVGEVLSLEDEDLIEEEEIVI
                     TLTNNGYIKRLANNEFRAQRRGGRGVQGMGIHNDDFVKNLVSCSTHDTLLFFTNNGKV
                     YRAKGYEIPEYGRTAKGIPIINLLGIDSSESIQAIIAVTGEAEEGHYLFFTTRQGTVK
                     RTSVTAFANIRSNGLIAIGLKEDDELVNVLLTDGQSNIIIGTHNGYSVTFKEEAVRDM
                     GRTASGVRGIRLREEDYVVGAALMKEDQEVLVITEKGYGKRTKVSEYPVKGRGGKGIK
                     TANITEKNGPLAGLTTVSGDEDIMLITDKGVIIRFNVSTVSQTGRSTLGVRLMKMEAD
                     TKVVTMAAVEPEAAEQTVKEQGSEE"
     gene            26902..27201
                     /locus_tag="IXT20_001251"
     CDS             26902..27201
                     /locus_tag="IXT20_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HAQ4859707.1"
                     /translation="MENTKYEIMYIIRPNIDEEAKTALIERFDAILKDNGAEVIESKD
                     WEKRRFAYEMNGYREGIYHIVKVSSPSSANAVNEFDRLAKINDDIIRHMIVKEEA"
     gene            27251..27763
                     /gene="ssb"
                     /locus_tag="IXT20_001252"
     CDS             27251..27763
                     /gene="ssb"
                     /locus_tag="IXT20_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HAQ4859708.1"
                     /translation="MINNVVLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQSGNR
                     EADFINCVIWRKSAETLANYARKGTLLGVTGRIQTRSYDNQQGQRVYVTEVVADNFQL
                     LESRSASENRQQSGGFQSSGQSAGGFGGNNNSNQTSQSSNGMPDFDRDTSDPFGSSST
                     IDISDDDLPF"
     gene            27789..28025
                     /gene="rpsR"
                     /locus_tag="IXT20_001253"
     CDS             27789..28025
                     /gene="rpsR"
                     /locus_tag="IXT20_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003129253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HAQ4859709.1"
                     /translation="MAQQRRGGRKRRKVDYIAANHIEYIDYKDIELLKRFISERGKIL
                     PRRVTGTGAKNQRKITVAIKRARIMGLLPFVSED"
     gene            28168..30141
                     /locus_tag="IXT20_001254"
     CDS             28168..30141
                     /locus_tag="IXT20_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HAQ4859710.1"
                     /translation="MENKNNHLSPKPLFLILFILIEVLVVLILPFRFVSASVILLMNL
                     WGVRYWLKMKRWLILNKREKIQQASTVAETNLAYVSEVMPVGVIAYQPSSQKIEWLNP
                     LALKIKDQSGLTEEELLDTFFTAKEKKKNQVYLADKTFQFDMDTVKGVVYFNDITQNQ
                     LLKLHQTNSQPVIGILSIDNYADAIDKLDDKEISYLNSFVTTLVSDWMNEYHVFFKRI
                     NAERFFFVAQMIDLKKMMDKKFDLLDRFRAEAEKQSSPITLSMGIAYGSRTLDEIGGE
                     AQNNLDVALVRGGDQVVVKEVKEGAKPVYYGGKSAKNVKRTRVRSRAMSTALRRIFDE
                     TGDVFIMGHKFPDMDALGSAFGVSCLARFNQKKAYIVINENEIIPDVERCLEEIHKHP
                     ELEMQLISPERAIELKKEEDLLVMVDYHKPSLSISQGLYEQFDKIVIIDHHRRGEEFP
                     SKPLLTYIESSASSASELVSELIQYQSSKTNRLDRFEATLLLAGMVVDTNSFNVRTTA
                     RTFDVASYLRSCGADASLVQYLLSSDLATYLEISNLIAGSDYVTSDIVVAAGTDEKEY
                     NSVIAAKTADTLLSMININAAFVITKRTDGLIGISARSTGTINVQIIMESLGGGGHFT
                     NAATQLENTTVEETKQKLFAAIKENMNQIYDKE"
     gene            30147..30599
                     /gene="rplI"
                     /locus_tag="IXT20_001255"
     CDS             30147..30599
                     /gene="rplI"
                     /locus_tag="IXT20_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HAQ4859711.1"
                     /translation="MKVIFLQDVKGKGKKGDIKEVPAGYAQNYLIKNNLAKEATKSSI
                     SQLAGQKKAQEKHEAEILEEAKKLKEFLEKEDTVVELKAKAGEDSRLFGSIPSKQIAE
                     GLNKQYKIKLDKRKIELENPIRSLGYTNVPVKLHHEVTATIKVHVVQE"
     gene            30873..32240
                     /gene="dnaB"
                     /locus_tag="IXT20_001256"
     CDS             30873..32240
                     /gene="dnaB"
                     /locus_tag="IXT20_001256"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HAQ4859712.1"
                     /translation="MNEVWQDRIPPQNIEAEQAVLGSVFLDAETIIDAMEYIEPKDFY
                     RRGHQIIFETMIELNDRNEAIDVVTVKDRLEQANLLEDAGGLSYLSDLALAVPTAANI
                     VYYAKIVEEKSLLRTLIQTATGIVTKGFEQGEEVQTILDDAERSILEVSEKRNRSGFL
                     SIAEVLNSTIENIDRLAQNNEEITGLPTGYQGLDKMTAGFQPEELIILAARPAVGKTA
                     FALNIAQNVGTKTDRSVAIFSLEMGAESLVNRMLCAEGSIEASHLRTGQLSEEEWSNL
                     IVAMGSLSRANIFIDDTPGIKISEIRAKCRKLAQEKGNLGLILIDYLQLIEGNGKENR
                     QQEVSDISRQLKKLAKELKVPVIALSQLSRGVEQRQDKRPVLSDIRESGSIEQDADIV
                     AFLYRDDYYEREGGDDDDGNEPKNDNVIEVIIEKNRSGARGTVELLFIKEYNKFSSLS
                     PREEF"
     gene            33020..34312
                     /locus_tag="IXT20_001257"
     CDS             33020..34312
                     /locus_tag="IXT20_001257"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HAQ4859713.1"
                     /translation="MSSVVVVGTQWGDEGKGKITDFLSENAEVIARYQGGDNAGHTIK
                     FNGVTYKLHLIPSGIFYKDKISVIGNGVVVNPKSLVKELAYLHEKGVETSNLRISDRA
                     HVILPYHIKLDQLQEDAKGENKIGTTIKGIGPAYMDKAARVGIRIADLLDKEIFEERL
                     RLNLDDKNRQFVKMFDSEPLDFDDIFEEYYEYGQQIKQYVTDTSVILNDALDAGKRVL
                     FEGAQGVMLDIDQGTYPFVTSSNPVAGGVTIGSGVGPSKINKVVGVCKAYTSRVGDGP
                     FPTELFDETGEQIREVGREYGTTTGRPRRVGWFDSVVMRHSKRVSGITNLSLNSIDVL
                     SGLETVKICTAYELDGELIYHYPASLKELNRCKPIYEELPGWSEDITGCKTLAELPEN
                     ARNYVRRISELVGVRISTFSVGPDRTQTNILESVWAQI"
BASE COUNT        10506 a         6694 c         6811 g        10416 t
ORIGIN      
        1 gaatcaagct tcgcaagtgg ttctttttta acgactcagt tatcttatca tgtcatttcc
       61 aacatgtcaa caacttattt tttattattt aataagttat tggtgttctt ttgaacaaat
      121 aataatatat caacatcaca tgtgaaagtc aataagaaaa tgcttattta tcaacatttc
      181 tagcttacat gatcattggt tagttctggt cctttttcgt tcgtctttat aactccgaga
      241 gacgtaaaat ccttttgttt tcagtttaat gttcatgttc ttgtcttatt cacaccaact
      301 agatgtctca ccttctaaaa ttttccttaa caaagtgctg cttttatcta tttctgcttt
      361 tttttgctag aataatagta acaatatgac tctggaggat ttatttatga aaattttgat
      421 tgctgacgat gataaagaaa tcgttgaatt attaagtatc tatgttcaca atgaggggta
      481 tgaagcagta aaggcttatg atgggaaaga agcattatct aaaatcagaa caatccctga
      541 tattgaatta cttattttag atatcatgat gcctgagatg gatgggatgc aagtagtgaa
      601 agagctacga aaagaatccc aaataccaat cattatgctg acagcaaaaa ctactgatat
      661 ggataagatc aaaggactgg tcgctggtgc agatgattat gtgaccaagc catttaatcc
      721 gttagaagta atggctagag taaagtcttt attacggcgt acaaaaatgc agattgcccc
      781 agatcagcct gatgtattag aagtaaatgc attaatcatc aataaagact ctcatgaagt
      841 taaaacaatc gatgggaaag aaatccagtt aacagctttg gaattcggta tcctctactt
      901 gcttgctagt catcctaatc gagtatttag cgcggatgaa atttttgagc gtgtttggaa
      961 acaagaaagt attgtctctg ctaaaactgt catggttcac gttagtcatt taagagataa
     1021 gattgaagaa gcaactggtg gtgaaaaagt cattcaaacc gtttggggag tgggctataa
     1081 aattgacgca agatagtaaa cgagcaactg aaaaacgccg tcggatcaat ttgacttcaa
     1141 aagaaatcag cgaattgtta gcggaaggaa tcatcacgat tatccttttg cttctattaa
     1201 atgtttctat tctggttgtt atcagttcgg tgatcaacag ttcaccttct ttgacgaatg
     1261 cgatttggga ttcaaagaat atttttgcag aacgattgaa cactgatttg ttctggaacg
     1321 gccggaattt tattattccc tttttctttc tattagatat tggtgttttg tattggcgct
     1381 tgattcgaag atatcgccaa atgcaattgc ggcatatcat cagcgaactt cactacatcg
     1441 caaacggaaa ttatgatcat cgtatccctt ttgaactgag tggtgactta agccgagtgg
     1501 taacaagtat caatggattg gtggatagta ccgtagcagc gattgaagac gaacgtaaaa
     1561 tcgaaaaatc aaaagatgaa ttgatcacga atgtcagtca tgatatccgt accccactca
     1621 cgtccatcat tggataccta ggactgatag aagatggcca gtatcactca gaagaagatc
     1681 ttttaaagta tacgcatact gcttatatca aagcaaagca gatgaaatct ttagtggacg
     1741 acttatttga atacactaag gtacgccagc ctgctgtccc ggtgaatttt tctgcctttg
     1801 acatgatcca gttgatcgaa cagttagcag ctgattttga gttggaagct tctaaaaaaa
     1861 atatccaaat acttgttcaa tcaaaagtag actcccttat catggatggc gataccgaaa
     1921 aacttgtacg tgtcttcaat aatcttttga cgaatgcgtt gaaatacgga aaaggagcaa
     1981 cgaagatcgt aatcgaagta gaacgtatcg gttcagaagt tgttgcaaca gtcaaaaata
     2041 acggagcaat gatcccacaa caggcaatcg ataatctatt cgaccgtttt tatcgtgtag
     2101 aagaatctcg ttcacaagca actggaggaa ctggacttgg tttagcaatc gctcaaagca
     2161 ttgtggcttt gcacggcgga tacatttatg caaaatcaga taagcaatgg acttctttca
     2221 tcatgcacct tccaatcaag aaaaatgaaa aattacctat tgatacacag gaagttattg
     2281 acgaaagctg aaatattcgc tattcttatt acaattatta gaataaatga aaaacacaga
     2341 acccagtaca ctttattctt tttcagagag ctgatggcag gtgtaaatca gcaaaggata
     2401 atttggaagt tccagttcgt ttacgtgccc tttcgaaata agaaaggtcg gttcaacagc
     2461 cgttatgtgt tcaacaagtg cagctgacgc tgaacttggg tggtaccgcg aatttcagac
     2521 tgattcgtcc caagaataat gggatggatc agtctttttt tattttcaaa aaggaggaaa
     2581 tcatatgtta gatgtaaaaa tgatgcgaca aaatattgaa gaagtacaag aaaaattgat
     2641 gactcggggc gtaaaaaaag aaacattagc tgaattcatg gaactagatg aaaatcgtcg
     2701 taagctacta gtcaaaagtg aagaattaaa aaaatatcga aatgatgttt ctgctgagat
     2761 cgcccaattg aagcgtgcaa aagaagatgc ttctgctaaa atcgctgaaa tgaaagaagt
     2821 cggtggcaac atcaaagcat tggatactga aattgctgaa gtggatgaaa aattgaaaca
     2881 aattgcaact actttgccaa accttcccca tgattctgtg ccagttggaa cagatgaaga
     2941 ggacaatgta gaaatccgtc gttggagcga accacgtaca tttgcttttg aaccaaaacc
     3001 acactgggaa attgcagaaa atcttgatat tttagatttt gaacgtggcg caaaagtagc
     3061 cggtagccgt tttgtctatt acaaaggatt aggtgctcga ttagaacgtg ctgtctacaa
     3121 cttcatgctt gatcagcata tttacgaaca cggctatact gaaatgatca ctccttatat
     3181 cgtcaacagt aaagcgatgt ttggtacggg acaatttcca aaattcaaag aagatgtttt
     3241 ccaattggag aatactgatc taacattaat cccaacagct gaagtaccat taactaacta
     3301 ttataatgat gaaattttag atggtgcaga tttacctatt tatttcactg cactaagccc
     3361 tgcttttcgt tctgaagccg gaagtgctgg tcgggataca cgaggactga ttcgtcttca
     3421 ccaattcaat aaagtagaaa tggtcaaatt ttcggatgta gagcattctt acgaagaatt
     3481 ggaaaaaatg acagctgatg cagaagatat cttgaaaaaa ttgcatttgc cttatcgtgt
     3541 gatggcctta tcaactggag acatgggctt ttctgcagct aaaacttatg acttagaagt
     3601 atggatacct gcacaagata cttatcgtga aatcagttct tgttctaact gtgaagattt
     3661 ccaagcacgc cgcgcaatga tccgttatcg cgataatgat ggaaaaatcc actatactca
     3721 tactctgaat ggttcaggac ttgcagttgg ccgtacagta gctgccattt tggaaaacta
     3781 ccaaaatgaa gatggttctg tcacagttcc agaagcgctc gttccttata tggggaatct
     3841 gaaagttatt aaataaagag gctgtgaaaa agccgcttag tttcgagtat taagaaaaat
     3901 tttgtgaaaa tggctttcca catttttgca taaattggac ttaataccga gaaactggct
     3961 tttgaacacc atttatcgag aggttgtgaa atcagcgaat taacctgagt attagagaaa
     4021 aaaatggaaa aatagtccct catattttac cgtttgttga actaataccg agggtcagct
     4081 gattgaacac cgtttagtgg agaggtcgta agcctacccc ccagctccta gttacaagga
     4141 caaattcaaa aaacagctct tcatatttta tgaatgttgt acttgtgatt cggaggtggc
     4201 aagggaacac tgtttattca aaaagaggct gggacagaag cgtttaactc caagaaataa
     4261 gaagaaattc acgaaaattg cttttcaaat ttttgtgaat ttcttcttat ttccgaagga
     4321 gttgcttcta cttccgccgt ttatacgtgt ttagagcgtg agacaaaagt gttctttact
     4381 tttgtcccac gctctttaat gtttttaaga ttcaactgaa tagtttggtg cttcttttgt
     4441 gatttgtaca tcatgagggt gtgattcttt taatccactg ccgctcattt ggataaattg
     4501 cgcttcatca cgtaattgct gtagattggc tgccccaaca taacccattc ctgaacgcaa
     4561 accgccgatc atttggaaaa taatatctgc aacacttcct ttataagcaa cccgaccttc
     4621 gattccttct ggtactaatt tatttgcttc attgacacta ccttggaagt aacggtcttt
     4681 tgaacctttt tccatcgcgc ctaatgagcc catacctcgg tatgttttga atcgacgtcc
     4741 ttggaagatt tcgaattcac ctggtgattc atctgtccct gccaacatac ttcctagcat
     4801 cactgcatgt ccgccggcag ctaatgcttt gacgatgtca ccagaatatt tgatccctcc
     4861 gtctgcaata atcgctttgc catattgacg agcaactgat gctgcatcat aaatagctgt
     4921 gagctgaggt acacctacac cggctaccac acgtgtcgta caaatcgatc ctggtccgat
     4981 accaactttt acgacatcta cacctgcatc atataaagct tttgtcgctt cagccgtcgc
     5041 aacgtttcct gcaatcaatg ttgcatctgc aaaagttgaa cggatttctt gaatttttcg
     5101 gatcacacct gcactatgtc catgagctgt atcgatgatg attgcatctg cccctgcttc
     5161 taataaagca ttcgcacgct caaatgtatc gcttgttaca ccaacggctg ctgctacaag
     5221 caatcgacca tgttcatctt ttgctgcatt tggaaattcg atcactttct cgatgtcttt
     5281 gatcgtgatc aatccgctta aacgaccttc gttatctacg attggtaatt tttcgatttt
     5341 atgtttttgc aaaatctttt ctgcatcttt tagagaggta cctactgggg cagtcactaa
     5401 gttgtctttt gtcatcacat cagcaatcgg catgtgataa tctgttacaa aacgcatgtc
     5461 gcggtttgta attatcccta ccaattttct gttttcaagt gtttcaacga ttggcacacc
     5521 actgatacga tatttgctca tcaattcttc tgcgtctgct acaagatgct gtggcgttaa
     5581 gaagaacgga tcaatgatta cgccactttc agaacgtttg acttttctta cttcatccgc
     5641 ttgctggctg atcgtcatgt ttttatggat cacacctagt ccgccttgtc ttgccatagc
     5701 aatggccatc ttgctgtcgg taacagtatc catacttgca ctgatgattg gaatattcaa
     5761 cttaatattt ttagctaatt gtacgctcat atccacttcg tttggtaaca cgtgactttc
     5821 tgctggtatc aataagacat catcaaaagt aaatcctttt ttcgtgaact ttgtttccca
     5881 gttggacatt tttagtacca ctcctctaat ttagtttatc tattaaaata attgattttt
     5941 tcatttaagt caatgatcta cttggaatca agttagcttt ctcatagaat accaatataa
     6001 agcgtttaca tctgtgtata ttaaaagaga ttgtaaaaaa gctgtcctgc atcaagtaat
     6061 aagaacagcc aatcaaaaac agcttctcat gtttttgaat tggttgggtt tattaccgca
     6121 gatgcaaact tttgaacacc gtttagtgga gaggctgtga aaaagctgct tagtttcgag
     6181 tagtaagaaa aattttgtga aaatggcttt ccacattttt gcataaattg gacttaatac
     6241 cgagaaactg gcttttgaac accgtttatc gtaaaaaggg actgtgacaa gacttttgtc
     6301 acagtccctt actattattt aactatggat aaacaaaatg aggttcttta ctacccctag
     6361 atatctagca aaatagctga tctttttgct agtttatctt cttgggacgg ataaacttcc
     6421 aataatcccg tattttttct tcaagtagta acggattgct aaagcagttg ccccgataat
     6481 gataactact actggatcta atactggatt gataactggt ggcaatagtg ttgtcgcaga
     6541 aaacagtacg acccaaagaa gtgtcgttcc acctaggata agcaatgtct tacccattcc
     6601 tggtttcttg cttcgatctg ctccgggata ttcatattga tagatgtatt tgtacatcat
     6661 ataaaagacc cagccaccga ttgcagaacc tagaacgagt gtagaaaagc cataagctgg
     6721 cgttgttcca cgtgaaaaca atccggtaat cgcaatcatg atacttaata gaccaagcaa
     6781 cagcaacgta ttatctaacc acatcaataa aggggtggat gaatttttta cttctgctgg
     6841 cttattgatg attgcttccg ttctttcaga gactgtccca aacaattgac gggctgttct
     6901 tccgcctttt tgttctgcaa ctaaagtagg cagaatgtca tgaagggctt ttactttttc
     6961 ttcttcagac aaattcgctg cttctaaaga ttttttcaaa tcaaaaatgt actgttgatt
     7021 tcttttggtt aattttgttt ctaaactatt gttttctttt acgattgctc gtaattgttc
     7081 tggttccatt atctaatagt cctccttagc cgttcctttt ctcaacggtc gtcacaaaag
     7141 acattctacc atattctaca gctagtttga tagccctttt acacattaaa gcggaataac
     7201 attacgtcgc cgtcttgtac gacataatct tttccttcta atcgaacacg tccagcttct
     7261 tttgctgcat gcatgtttcc gtaatgatcc aagtcttcaa aagagacagt ttctgcacgg
     7321 ataaatccgc gttcgaaatc tgaatggatg attcctgctg cttgtggtgc tttgattcct
     7381 tttttgaatg tccaagcacg tacttcttgt tccccagcag taaagtaagt agctaagcct
     7441 aacaaatcat aagcagcacg gatcaactga tccaaacctg attcttcgat tcctaaagct
     7501 tctaaaaatt ccgctttgtc ttcttcatct aattctgcga tttcttcttc tgcacgagca
     7561 ctgaccacga tcacttctgc attttcttct gcagcaaagt tgcgcacttc ttgtacataa
     7621 tgattcgcgt ctgcatctgc tacgtcatct tctgagacat tggctacata aagaattggt
     7681 tttgtcgtca ataaaaatag tgatttaacg attttttgtt cttcttcagt aaattcgatc
     7741 gttcgggcag attttccttc ttccagtaca ggtttgattt tatccagtac agctagttct
     7801 gccactgcat ctttatcttt ggttttagca acttttgcta cacgagtata gcgtttgttg
     7861 atagattcta agtctgctaa cactagttcc aagttgatcg tatcaatatc ttctagtgga
     7921 tcaacacgtc cttctacgtg agtaatatta tcatcatcaa aacagcgaac aacgtgacaa
     7981 atagcgtcta cttggcggat atggcttaag aattgatttc ccaaaccttc tcctttactt
     8041 gcgcctttta cgattccagc aatatctgta aattcaaaag tcgtcggaac agtttttttc
     8101 ggatgtacaa gttctgttaa gcgttgcaaa cgccagtcag gtacttctac catccctacg
     8161 ttggggtcga tcgtcgcaaa gggatagttt gctgcttctg ctcctgcttt tgtgatcgca
     8221 ttaaacaaag ttgattttcc tacgttgggc aacccaacaa ttccagcagt caatgccata
     8281 aatttattca ctctttctct gcattttttt caattacttt ttttaatttc ttttcaaaat
     8341 ctcgacgggt catcatgacg atatgtccgc aattcataca tctgattttg atatctgcgc
     8401 ccattcgaat gacttcccag cgattcgtct ggcaagcgtg aggttttttc atttcaacga
     8461 tatcgtgcaa gtcgtacata aaaatagcct cctttttgtt tattcttcat caaattgaat
     8521 attcaagata tcaagaattc gagtcaaatc tttttgagaa agatattcaa tctcgatctt
     8581 acctttccca tttttttctt ggatagctac acttgtacca aatttatcca tcaaacgatc
     8641 ttcgctttct cggatataat atggtttttc ctttgctagc cttgggattt ttttcttttc
     8701 ttttccatca ttttgattta actcagaaac aagctgttcc agttggcgga cagttaaatt
     8761 ttcagttact gcacgattgg ctaatttcaa gatcaatgtt ttatttttta aacctaacag
     8821 cgtacgggct tgtcccatag ataaacgctg atcttgtacc atttccttga caagatccgg
     8881 taaagaaagc aagcgcaaat aattggcgat ataaggtctg ctctttccta aacgactagc
     8941 tacttctgct tgtgttagtt tcaggttttt catcagcatc tcatatgctt ctgcttcttc
     9001 taacggattc agatcttctc gctgcaagtt ttccagcact gctacttgca tcatcgcttc
     9061 ttcgtcaaag tctctaacga tagcagggat cgtttctttt tcagctagtt tggacgctcg
     9121 aaaacgtcgc tcccctgcaa taatctcata tccttttact gcggaacggc gtacgatgat
     9181 gggttgaaag acacctgatt gttcaataga attagctagt tcctgcaaag atacttcttc
     9241 aaatgtttta cggggctgat acggatttgg tcgcagctca ttcaatggta gctggacaac
     9301 ggtatcatct tttacatcca ccgtctctaa gttttctaaa tcttgaaaca gtgcatcgat
     9361 tcctcggcct aatcctttac ttttcttcac gacttaccac ttcctttgcc aatgcttggt
     9421 agacttcagc tcctcgagaa cgtggatcgt agtcgatgat cggttttcca tgactaggtg
     9481 cttctgataa acgaatattt cttggaatga tcgtttcgta aactttttcg cggaagtatt
     9541 tacgtacttc ttctacgact tcatttccca gatttgttcg tgcatcatac attgtcagta
     9601 agactccctc gatctctaat tctggattaa agtgtttttg gactaaacga accgtattca
     9661 gtaattggct taatccttcc aatgcgtaat actcacactg aacaggtatc aaaatcgaat
     9721 cacttgctgt aaatgaattg attgtcagat ggccaagaga aggcgggcaa tcgatcaaaa
     9781 tataatcata ctgactgctt acttctgcta aagatccttt taatcttgat tctcttgcca
     9841 tcattgacgt caattcgatc tctgctcctg ctaattggag agtcgcaggc acaatactca
     9901 aattttcgtg ttcagtgatc aaagttgcct catcaattgg caattcattg actagaacat
     9961 catagatgtc tcttgtcaca tcaggttttc tgatcccgac accactggta gcatttcctt
    10021 gtgcatccat atccactagc aatacttttt tgccaaggct agcaagacaa gcacctagat
    10081 tcaccgtcgt cgttgttttg ccgacgccac ctttttggtt cgcaacagat attatttgtg
    10141 ccatctcttc tcctcctatt tgattggctg cttattaggc agtccgggct ttcttgggta
    10201 tttctttggg gttggtttct ttttttgaat aacgatgata tgccgttgat cagagacatt
    10261 cggcaaggta accgcctctt ctttgaggaa tttaccacct aataaagcaa tcgcttgttt
    10321 tgcttctgtc aattcctctt cactttttgc tgctttcaat gcataaaaat agccttcttg
    10381 tttagccaag ggcaaacaca attccgctaa cacattcaaa cgagcaaccg cacgagcagt
    10441 gacaaggtca aatgatgcac gaaagtgagg attttgccca aatgtttctg cccggtcatg
    10501 atataaggaa gcatcgattc ctaataatcg ggttaactca gataagaagg ttattcgttt
    10561 attcagagaa tcaacgatcg ttatctgcag ttctggaaag gcaattttta aaggtatgct
    10621 aggaaaccct gcccccgaac caacatcgca taaagataaa ggttgcgtaa agtctacttc
    10681 aaatgctaat gtaagtgaat cgtaaaaatg cttcagatat acgtcttttt catccgtaat
    10741 agctgtaagg ttcatctttt cgttccattc gactaatgtc cgataataaa tagcgaattg
    10801 attcatttga tgatcggtaa gctctatccc tttttctgcc aaggattggc gaaattcttc
    10861 tggtatcata aatattcctt tcattgtgaa acagcaattg tttcacagat tctctcccta
    10921 ctttaacgca aaatctctag acatgcaatc cttccatatg gattcttaaa aatttgaaaa
    10981 gtaagaaaag ccgatagact ttttctttct agtatgactc ataaaatata cacagaagag
    11041 agaactagct ttgagcaatt tctgcaaaaa ctagttctct cttctattta ttattacgta
    11101 ttacgctggt tcactgactt tcgcaatctt cccttgttcg atatagacca ttaggatact
    11161 taggtctgca gggtttacgc cactgattcg gctagcttgg gcaattgttt ctggttggat
    11221 tttctgcaat ttttgttttg cttctgttgc taatccgttg attgctgcgt agtcgatgtt
    11281 ttctggaatc tttttcgctt ccatgcgttt taatttttct actttttcca tggctttttt
    11341 gatgtagcct tcatatttga tttggatttc tacttgttcg atttcttttc gtgtcaattc
    11401 ttcattttct ggaataaacc gtagaagttc tgcataagta atctcaggac gtcttaaaaa
    11461 gtcgcttgct agtacaccat cttttaatgg tgccgcatta atcgttgcta aaaagtcttg
    11521 gacttcttct gttggtttga ttcgtacatt catcaaacgt ttgatttctt gttcaactgc
    11581 ttcttttttc ttcaaataat tcttgtattg atcttctttt actaagccta cacggtgacc
    11641 catttctgtt aatcgcaaat ctgcattatc atgacgtaaa agcagacggt attcagcacg
    11701 tgaagtcagc aaacgataag gttcgttcgt tccttttgtt accaaatcat cgatcatgac
    11761 accgatgtaa ccgtcacttc gtttgatcac taaaggttct tttccctgaa ctttcaaagc
    11821 tgcattgata ccggcaatca gcccttgtcc agccgcttct tcataaccgc ttgtaccatt
    11881 tgtttgtcca gctgtataaa gattttcaac gactttagtt tctaatgtcg gacggagctg
    11941 gtgtggctct actacgtcat attcgatagc atatcctgta cgcatcattt ctactttttc
    12001 taatccagca atagaatgaa gcatgttgac ttgaacatcc tctggtaaag aagtagataa
    12061 accttgaaca tagacttctt ccgtatttaa gccttctggt tccaaaaata attgatgacg
    12121 tggtttgtct gcaaaacgca cgatcttatc ttcgatggat ggacaatagc gagcgcctac
    12181 cccttcaacg atccctgtaa acattggggc acgatgtaag ttgtctcgaa taatttggtg
    12241 agtcgtctca cctgtatagg tcagccagca agattcctga tctagtttat aggcactatc
    12301 tggtgtgctg aagctaaaat gattcggctc tttgtcacca ggctgctctt ctgtaacaga
    12361 atagtcgatc gtacttgatt ttactcgagg cggtgttcct gttttgaacc gttcgatagc
    12421 aaagccaagt tcttttagat gattcgctag tccaatagaa ggttgagagt tattcggacc
    12481 tgatgaatat ttcaattcac cgataatgat ttctccgcga agtgctgttc cggctgtaat
    12541 aacaactgct ttggcccggt agctcgctcc tgtggacgtc actactccat gacaaacacc
    12601 atcttcgacg atcagttctt cgacgattcc ttgtcttagc gtcaaatttg gttctttttc
    12661 gatcgtatgt ttcatttctg ttgaataagc atgtttgtct gcttgtgctc gcaatgctcg
    12721 gacagctggg ccttttcctg tgttcaacat acgcatttgg atataggttt tatcgatatt
    12781 cttacccatt tctccgccta acgcatcgat ttctcgaacg acaacgcctt tagctggtcc
    12841 gcctacagaa ggattacatg gcatgaatgc taccatgtcc aaatttattg tcagtaataa
    12901 tgtttttgat cccatacgtg cagcagcaag tgcagcttct gaaccggcgt gtcctgcgcc
    12961 aacaacgatc acgtcatacg catctgcttc ataatgttcc aatcgtttgt tcctcctttt
    13021 attttcctaa acaaaattga ctgaataatt gagtgattag ctcatcttgt acactgtcgc
    13081 cgacaacttc tcctagatag tcccagcaac gcgtcatatc tatttgaacg agatccacag
    13141 gcattcctga atcaatacct gcaatgactt cttcaagtga gagggaagct ttttctaaca
    13201 acgcgatatg gcgggtatta gaaacataag tagcgtctct ttctcctgtt tctccgctga
    13261 agaataaatc agaaatagct gattccaaac gatctagtcc gtcgttttta gctacggaaa
    13321 ttgaaaaaac tggttcattt tcgatcaatt tcttcaattt ttcttgttcc agctgtgctg
    13381 gcaaatctgt tttatttaga agaatgatcc gtttcaatcc acttgtcgct tccaataatt
    13441 gttcatcttc tgctgtcagt ggttcgcttt ggtttagaac aagtaagatc aagtctgctt
    13501 ctgctaatgc ttttcggctt ctttcgaccc cgatccgttc caccacatct tctgtttccc
    13561 gaattccagc tgtatcgatt agcttgagcg gtaccccccg aacattgaca tactcttcga
    13621 ttacatctcg agtcgttcct gcaatatccg taacgattgc tttttcttct cttaacaaat
    13681 ggtttaacaa acttgatttg ccgacatttg gccggccgat gattgccgta cttaaccctt
    13741 cccgcagaac cttcccttgc ttggatgtag caagtagcgc ttggattctt tgttggatca
    13801 tctgcgcttt ttcaagcaat aacttcgtag tcaattcttc gacatcatca tattctggat
    13861 aatcaatatt tacttctact tgtgctaatg tctctaaaat ctcttgtctc aatgagcgga
    13921 tcaaagcaga cagattgccg tctaactgat taagagctag tcccattgct ttgtctgttt
    13981 ttgcccgaat caaatccatc acagcttctg cctgtgaaag gtcaacacga ccattcaaga
    14041 atgctcgttt tgtgaattcg cctggctcag ctaatcttgc accttctctt aacaatagct
    14101 gcaaaatttg attgactaca acgatcccac cgtgacaatt gatttctact acatcttccc
    14161 gcgtaaatgt ctttggtgct ctcatcacgg agaccatgac ttcatcgaca agctgattgc
    14221 tcttgggatc aacgatatgt ccataatgga tcgtatgact tggtacttct gataatcgtt
    14281 tgtttccaga ttgataaact ttatctgcta gttgcacagc ttgatcgcca cttaaacgaa
    14341 cgatactgat tgcaccttct ccaggtgggg tagaaattgc cgcaatcgta tcaaattcta
    14401 aagtaatttc agccattttt tcacttcctt tttcattttg cgcacaaaaa aagtgcccac
    14461 tccacccttt atgcacaata aacggtgaac ttcgcacttt gactgcttgt tcttgattaa
    14521 attctggtca tagattatca cacttgctat aaggaatcaa gtgtttaaaa caattagatg
    14581 aagttgagcc tgttctgctt ccattacctt atatttaatt gtccttggat gtctctttgc
    14641 gttctttggt ttattcgaag agaagatact gtataaacaa ggctttatct ttcttaaaaa
    14701 aaacggtaca ctaaatggta gcgctatcta aactatacac ttttgaaagg agaaaatatg
    14761 tgagcccgtt cagatctgct gcattggaag aagaaagtga ccctcggatc aacgatgtcg
    14821 ttttatctac agggaatatc aaccagaaat atatcgtccg cgatatcgtt tttgttgtgg
    14881 aatgcttaga agctgatttg tttgacccat cttttcccaa agatgacctt ttcttaacaa
    14941 cgaagaggaa attaaagacg aaggtacttg aatatggtgc gaatgcagta atcaactgtc
    15001 attttgagta tcagcaaaaa aaacgcgacg gaaagtattt tctggaactt tttgcttatg
    15061 gaacagtgat ccaatttatc caaacaacga ttggttaaat gaaacagaca aaaaaagcgg
    15121 atcaaaactg aagacagttt cgatccgctt ttttctatca aagttagtga ctacttcatc
    15181 gtatactctg ataagaatga tttctagtaa aatatacttg gtttgaaaaa ctttgtcaac
    15241 gtgatttatt caaaaagtaa tggaatcaat aatatttttt agcaggttct actactaaat
    15301 aacgataagg ttcctcacct tccgaatgtg tttttacatg ttcatctttg cttaatgcag
    15361 catggatttg tttacgttca aatgcaggca ttggttctaa gaaaacaggc tgccctgtcc
    15421 gttttacttt ttcagccgtg cgatcagcta aacgctgaat gatctcttgg cgcttttcac
    15481 ggtaattgcc tacattgact actacagaca gcttatttct tgctaaacga tggatgaaga
    15541 cttgtgccag atattgcaat gcattcagtg tttttccatg tttcccaatc agtatgcctt
    15601 gcttttgtgt ttctaaatgg aaaacaacca tattatttga ttctctttcc attttgacaa
    15661 gagctggagc atcaagctgt tttgaaattt cagtaagata aactgccaat tccgttaaag
    15721 ctgcttcatc acctagctca cttacttctt ctgttttttc ttcaagaatc gtttcgttga
    15781 ctacctcgat ctcagctgtt ggttcttctt ccaaaatttc ttctacagtc tgttctactg
    15841 tttcagaaac tttttcgctg atacttggct ggatcgaaag ccgtgcattc tttcgaccca
    15901 ttcctaggaa accttttttt ccttcttcca gtatttcgat ttcgacttgg tctttggcca
    15961 aacccaaatc agccagacct agttggacag cttcgtctat tgttgcagct tcataaatcg
    16021 gcattagcat tacctcccct attatttccg ttttttctta ggattcattg ctttcttaag
    16081 agcacgttct ctttcgcgga ttctccgtgc ttcttcttca cgctcttttc gaattttgaa
    16141 agggttgttg atcagcagcg tttgtacaac ttggaaggca ttggaaacta cccagtataa
    16201 tgaaagaccg ctggctaaat tgacacccat cactaaaatc attacaggca tcacgaaatt
    16261 catgatcttc attgatgcat tcgattcaat ttgactcatg ctagataaat acgtacttgc
    16321 aaaagtaaag attgctgcta aaatcggcaa aataaatgtt ggatccgcgt tccctaagtt
    16381 caaccataag aaatgccctt gtttcaactc tggtacacgt gaaatagatt gccataacgc
    16441 catcaagatt ggcatttgta ctaataaagg aagacaacca gcatatggat tgacgccatt
    16501 ttctgcatat aaacgctgct gctcttctct taacttactt tgtgtttccg gatctcttga
    16561 actatattgt tgctgcagtg cttttaattt cggttgtaat tcttgcgttt tacgcatgct
    16621 ctttgtttgg aaatgcatca acggcaatag gatcacgcgg atgatcagtg taaataaaat
    16681 gatccctatc cctgcattac caaatgacaa cgccttgatc gcctcagcaa agtaataaac
    16741 aatataacga tcccaaattc ctgtactctg tgcgctgact tccccagttc cgcaaccgga
    16801 taagacgatg accaaactaa cgataccggc catcaaaagt aaacgtttgt atttcttcac
    16861 ttactctttt cctctctgtc gattattttt gcaagtttta agacgtggac taaattggag
    16921 cgaacctcat cataagtcaa ccccttcaca cttggtctag caatcacaat aaaatccaac
    16981 tccggatcaa tttcttcttt tatagaataa actgctgctc ggatttttcg tttgacttca
    17041 ttgcggttta ccgcgttgcc gacctttttt cctacagata aaccaactcg aaaatgaggc
    17101 tgtcccggtt tttccaaacg gtaaacgacg aatttgcggt tagcaaaaga atctcccgta
    17161 tggaacacgg cttgaaactc tcgttctttt tttactcgat acgcttttct cattagtttt
    17221 ctttcctttt ctatctctca cttacgctca taatcatacc ataattacga ttgtcctttc
    17281 atcatttccc taaaaaagag tataaaaaaa accactgaaa ccccagtggc ctaagctgaa
    17341 agaacttttc ttcctttgcg acgacggcta gctaacacac gacgtccatt tttagtactc
    17401 atacgtttac ggaatccgtg aactttttga cgtttacgtt tatttggttg gtatgttctt
    17461 ttcatatatt ccacctccaa aatgttctta tctattttat aaacacagac atactttgat
    17521 agtataacga caaagtagtg attttgtcaa tataaaatag aagatcgatt gttaaaactt
    17581 atccacaaga ttattttttt ctatgggaat cctatgtgga aaaagttatg cacagggatt
    17641 atccacagat aataaaaggc tggttttaca cagaaaacac acgtgtaaaa aaagtaatcc
    17701 acaatcaggc gatttcctgt gtattccgtc tcctaactat tggtatatct gtatttttta
    17761 tccacagacg caagaggaaa tctattggtt tttacgagtt ttccacctgt gcattagtgg
    17821 tgtggaaaac tttttttgac aactgtgttt ttctcttttt tctttttgtc gattgtgtgg
    17881 ataattgtaa ttaaaaaatt tttttcttcg gtttctgtgg aaaactattg aaaaaaatgc
    17941 taaattagta ctagttattc actttttaat gaaggaggag gccttatatg gtatccctcg
    18001 atgctttatg gaatgaatta aaagcaacat accaaaagga tttatcgcct gctagctata
    18061 atacatggat cgaaacagcg aaccctcgaa cccttgatca aaaccagctc gttgttgagg
    18121 ttcctagcaa gattcataaa gaatattggg aaaaaaacct ggcgacaaaa atcgttgaag
    18181 tcggttatat gttatcagga aatgagatta tccctcgctt tattactggc gaagaagcag
    18241 agcaagaaga agttatagaa gaaaaaaatc caaaagttgt ggcacctagt ccgctaaaaa
    18301 aagccatgct gaaccctaaa tatacctttg atacgtttgt cattggtaaa ggaaaccaga
    18361 tggctcatgc agcagcgctc gttgtagcag aagatccagg ttctatttat aacccgctct
    18421 tcttttatgg aggcgtggga cttggtaaga ctcacttgat gcatgcaatt ggtcatcaaa
    18481 tgctgcaaag tcagcctaat gccaaagtaa aatatgtaag cagtgaaact tttgctaatg
    18541 atttcatcaa ttcgatccaa aacaaaacag cagaagagtt ccgtcaagaa tatcgaaatg
    18601 ttgatttact gctagtggat gacattcaat tttttgcaga aaaagaagct acgcaagaag
    18661 agtttttcca tactttcaat gccttgtaca atgaagggaa acaaatcgta ctaaccagtg
    18721 atcggttgcc aaacgagatt ccgaaactgc aagaacgcct tgtttctcgc tttgcatggg
    18781 ggctgtctgt tgatattaca ccgccggatc ttgaaacaag aacagcgatt ttacgtaaaa
    18841 aagcggcggc tgaacgtcta gagatcccag atgacacact aagctacatc gcaggacaaa
    18901 ttgattctaa cattcgtgaa ttagaaggtg cgcttgttcg agtgcaagct tttgctgcga
    18961 tgaatagtga agatatttcg actagtctag ctgcagacgc actgaaaacg ttaaaatcag
    19021 gaaaaggaca tcctcaattg tcaatcctgc agatccaaga agaagtggca aagtattatc
    19081 acatccaatt gaaagatctc aaaggaaaga aacgcgtcaa atctattgtt gttcctagac
    19141 aaatcgctat gtatttggca cgtgagctca cagataattc tttaccgaaa atcggagctg
    19201 aattcggtgg caaggatcat acaacggtta tccatgctca tgaaaaaatc cagcagctga
    19261 tggattctag tgtcagtatg caaaatgaag tatcagaaat caaaaacttt ttactaaatt
    19321 aatttgtcga ttctatcaac tgaaaatagc atgtggataa aagaaaaaaa cgttcaaaag
    19381 ttatacacac tttttccaca gctgtttttt cgtttctctc ttacgttttt tgagttttcc
    19441 acagaatcaa catgccctat tacttttatt attcttttta actaataata aataaacaaa
    19501 cattcaaagg agatacatca tgaaagttac tttaaaccga gctagcttta tgcaggaatt
    19561 gcaaactgtt caacgagcta tttcaagcaa aaccacgatc cctattttga caggtgtaaa
    19621 aatcacactg acacaagaag gtttgacttt gacggggagc aacgctgata tatcaattga
    19681 aacttttttg tctgttgaaa acgaaaaagc aaatatgcaa atcgaatcta ctggttccat
    19741 tgttttacaa gcacgtttct ttagcgaaat cattcggaga cttcctgaag aaacatttac
    19801 tttagaagtt ttagaaaata aacaagtagc gatcacttct ggaaaagcga attttatcgt
    19861 aaatggatta gatgcagata actatcctca tcttcctgtt gtcgaaagcc ataaccagat
    19921 gaaattacct gtacacgtat tgactaaact aatcaacgaa acagtttttg ctgtctcaca
    19981 acatgagagt cgtccaatct tgacaggtgt gcatttcatt ttatctgata attctttatt
    20041 agctgtagct actgattctc accgtctaag tcaacgcgtg attccagtag aacaagcggc
    20101 tgatcatttt gatattgtta ttcctggaaa aagtttgatc gaattatctc gctcattaac
    20161 aaatgaagaa gaaatcgtcg aaatcagcat tatggaaaac caagtgttgt tcaaaacaga
    20221 aacgatgtat ttctattccc gtttgctaga aggaaactat ccagatacca atcgtttgat
    20281 tccttcaagt tttaatacag aggttgaatt ttctgttcct agctttttag cggcgatcga
    20341 acgggcttct ttactttctc atgaaggccg taacaacatc gttcgtttat ctattcgtcc
    20401 agatgcggtt gttttatacg gaaattcgcc agaaatcggg aaagtagaag aaagcttgag
    20461 ttatacagca agcagtggcg atccattaga tatttctttc aatccggatt atatgaaagc
    20521 agcgttgcgt gcatttggag atatgagcat caaagtgaaa tttatctctg ctattcgtcc
    20581 atttacattg gaaccaacgg aagatggcgt tcaatttatt cagctgatta ctcctgtacg
    20641 tacaaattaa caatgatttt caatgttctt gaaaatttaa ttgaaaagaa atagaaaaaa
    20701 taaaatttta aacagaaggc cctaaaatct cttttaaggc tttctgttta tttttgttta
    20761 aatcaccttt tttagattaa atcgtcttat atggaaaaat atatggataa atttgtttta
    20821 ttgcgaaaaa aaaggtataa tagagtatac ttttaataat gaaaatgagg gatgggcatt
    20881 gaaacaacaa gtcattctaa atacggaatt catgacgttg ggccaaatgt tgaaagaagt
    20941 cacagttatc ggtagtggcg gacaagcaaa atggtatttg gcagaaaaca ctgtgttagt
    21001 cgatggcgaa ccagaaaatc gtcgtggacg caaactttat cccggcatga tggttgaagt
    21061 tccagaagta ggaacttttt ttatggtaaa aaaaggaaac gacgataatg aggctgaata
    21121 agctgtattt aaaaaattat cgaaattacg aagagctgaa catcgaattt tccaagaacc
    21181 tcgtcatctt tttaggagaa aatgcgcaag gaaaaacgaa tcttttggaa agtatctacg
    21241 tattagctat gacgcgaagt catcggacaa gtagtgagca ggaattgatt ggctggactg
    21301 acgatcaggc gatgattcaa ggagagatca caaaaggttc atccactctt ccattagaaa
    21361 tcttattatc taaaaaaggc cgaaaaacca agatcaacca tatcgaacag aaaaagttaa
    21421 gtacttacgt gggacaaatg aatgtcattt tatttgcacc agaagatctt tctttagtaa
    21481 aaggcagccc gcagaccagg cggaagtttt tagatatgga attaggccag atcaatccaa
    21541 tctatcttta tgatcttgtc caataccaag caattttgaa acaaagaaac cagtacttga
    21601 agcaattgaa tgaaaaaaaa caaactgatt tgctttattt ggatattttg tccgaacaac
    21661 tagcagcgtt tggcagcaaa gtattgaaag caagagcaca gttcgtcaag cgattggagt
    21721 tttgggctaa ttctttgcat cagcaaatca cccatcaaaa agagcagctg gaaatcgagt
    21781 atctcaccgc agtcgattct ttagaaacac atacgcaaga gcaaatccaa gaacaatttc
    21841 tcgcactttt gaaccaaaat aaaaagaaag acctttttcg gggaacaacg acagttggtc
    21901 ctcatcgtga cgatctttcc ttttttatta atcagaaaaa tgtgcagact tatggatcgc
    21961 aaggacagca aagaacaact gcactaagtg tcaaattagc agaaatcgat ttgattaaag
    22021 aagagacagg ggaatatcca atccttttgt tagatgatgt gatgagcgag ctggatgata
    22081 atcgtcaact ccatctgcta gaaacaatcg aaggaaaagt tcaaacgttc ttaacaacaa
    22141 cgacacttga ccatgtaaaa aataaaatga ctgttgaacc tgagattttt tatgttcaac
    22201 aagggaaact aaaaggagaa gaaactgaat gacagaagaa agaagtttag tagaacgcgc
    22261 taaagagtat gatgccagtc agattcaagt acttgaagga cttgaagccg ttcgtaaacg
    22321 acctggcatg tatatcggtt cgactagttc tgagggtctg catcaccttg tttgggaaat
    22381 tgtcgataat tcgatcgatg aagtattagc aggttttgca acgaaaatcc atgtgattat
    22441 tgaaaaagac aacagtatca ccgttattga cgacggtcgg gggatacctg tagatatcca
    22501 ggcaaaaact ggacgtcctg cagttgaaac ggtctttact gttttacatg ctggaggaaa
    22561 attcggcggt ggtgggtaca aagtttctgg tggacttcac ggagtaggat cttctgttgt
    22621 taacgcgctt tctacacagc ttgatgtcaa agtatacaaa gaaggaaatg tgtactatca
    22681 agaatatcgt cgtggagctg tagtggacga cttaaagatt atcgaacaaa cggatcgcca
    22741 tggaacaacg gttcacttta cgccagaccc tgaaattttt acagagacaa ctgagtttga
    22801 tttttcaaaa ttggctaccc ggatccgtga acttgctttc ttgaaccgcg ggatgcgtat
    22861 atcgattgaa gacaaacgag aagaagaacc agtcatcaat gaataccatt atgacggcgg
    22921 gattaaaagt tatgtcgaat acttgaatgc aaacaaaacc gtgatttttc cagaacctgt
    22981 atatttggaa ggtgaacagc aagatatcgc agttgaagtc tctatgcaat atacagacgg
    23041 ctaccattcc aatatcatga gttttgccaa caatatccat acgtatgaag gcggaacaca
    23101 tgaatcagga ttcaagactg ctttaactcg tgtgataaat gattatgccc gaaaacaaaa
    23161 gctgatgaaa gaaaatgacg acaacttgac cggtgaagac gtgcgagaag gattgacagc
    23221 tgttatttct atcaagcatc ctgaccctca attcgaagga caaacaaaaa caaaattggg
    23281 caattccgaa gtacggacag tcactgatcg gttatttagc gaacatttca tgaaattctt
    23341 gctggaaaac ccaagtgtgg gccgtcaaat tgttgaaaaa ggattactag cttccagagc
    23401 tcgtctagca gcgaaacgtg cgcgtgaagt aacgagacgt aaaggtgctt tagagattag
    23461 taatctgcca ggaaaattag cggactgctc tagcaacgat cctgaaaaat gcgaattgtt
    23521 catcgtcgaa ggagattcgg ccggcggatc agctaagcaa ggtcgtaatc gagagtttca
    23581 agccatcttg cctattcgtg ggaaaatctt gaatgttgaa aaagcaagca tggacaagat
    23641 cttggcaaat gaagagatcc ggtctttatt tactgctatg ggtacagggt ttggagaaga
    23701 ttttgatgta tcgaaagctc gctaccacaa attagttatc atgactgatg ccgatgtcga
    23761 cggcgcgcat atccgtacat tgctgctgac tcttttttat cgttacatgc gtcctattgt
    23821 tgaagctgga tacgtctata ttgctcagcc acctttatac ggggtaaaac aagggaagaa
    23881 tattacgtat gtacaaccag gaaaaaatgc agaagaagag ttgaaacaag tcgttgcttc
    23941 acttcctgcc tcaccaaaac caagcgttca gcgttataaa ggacttggag aaatggatga
    24001 tcatcaacta tgggaaacaa ctatggaccc aagcaatcgg atgatggccc gcgtcagtgt
    24061 ggacgatgct attgcagcag atcagatatt tgaaatgttg atgggggatc gagtagaacc
    24121 ccgtcgtgca ttcatcgagg aaaatgcaca ctatgtgaag aacttagata tttaatcatg
    24181 aaggaggaaa aagaatgagt gaagaaatca gagaaaacat ccatgatgtc aatctaacca
    24241 gcgaaatgaa agactccttc atcgattatg cgatgagtgt tattgtcgca cgtgcacttc
    24301 ctgatgtcag agatggtctg aaacccgttc atcgccgtat tttatacggc atgaacgaat
    24361 taggtgtaac accggataaa gcccataaaa aatctgccag aatcgtcgga gatgtcatgg
    24421 gtaaatacca tcctcatgga gactatgcga tctatgagtc aatggtacgg atggcacagc
    24481 cattcagcta ccgttatatg ctagtggatg gtcatggaaa ctttggttct gttgacggtg
    24541 acggtgccgc cgctatgcgt tataccgaag cacggatgag caaaatcgct acagaaatgc
    24601 tgcgtgatat caataagaac actgttgatt tccaaagcaa ctatgatgat acagaaaaag
    24661 aaccagttgt actacctgct cgcttcccga atcttttagt gaacggaaca acggggatcg
    24721 ccgtaggtat ggccacgaat atcccacctc ataacttgtc tgaggtagta gcggctatcg
    24781 atttgctgat ggataatcct gatgtgacga ccaatgaatt gatggaagta ttacctggac
    24841 cagattttcc aacaggtggt ttggttatgg gaaaatcagg aatccgaaga gcgtatgaaa
    24901 caggtaaggg ttctattacc gtacgtgcaa aagttgagat tactgagatg ccaaatggta
    24961 aagaacggat catcgtgaca gaattgcctt atatggtcaa taaagcaaaa ttgatcgaac
    25021 ggatttctga actgcacaga gacaagcgta tcgaagggat cacagatttg cgggacgaat
    25081 cttcccgtga aggtatgcgg atcgtcattg acatccgtcg tgacgccagc gcatctgtta
    25141 tcttgaataa cttatataaa ttgacatcct tgcaaaattc atttggattc aatatgttgg
    25201 cgatcgaaaa aggtgtgccg aaagttttga gcttgaaaca aattcttgaa aactatatcg
    25261 aacatcagcg cgaagtcatc acaagacgga cagtctttga aaaagaaaaa gcagaagcaa
    25321 gggcacacat tttagaaggt ttgcgtattg ctttggatca tatcgatgaa atcattgcga
    25381 ttatccgcgg gtctaaatca gatgacgaag caaaagcgac gatgatcgaa cggttcgatt
    25441 tatccgatcg ccagtctcaa gctattttag atatgcgttt acgccgattg accggtttgg
    25501 aacgggaaaa aatcgaaaat gaataccaag aactcttaaa actgattgaa gatttaactg
    25561 acattctggc aagaccagaa cgtgttacag aaatcatcaa gaatgaatta gcagacttga
    25621 atcaacgatt tggtgataaa cgacgcacag aattactagt cggtgaggtc ttaagtctgg
    25681 aagatgaaga tttaatcgaa gaagaagaaa tcgttattac attgacaaac aatggttaca
    25741 tcaaacgttt ggcaaataat gaattcagag ctcaacgaag gggtggacgt ggtgtccaag
    25801 gaatgggtat ccacaatgac gacttcgtca aaaatctggt ttcttgttct acccatgata
    25861 cattgttatt ctttacgaat aacggaaaag tgtatcgggc aaaaggatac gaaattccag
    25921 aatacgggcg cacagccaaa ggaataccaa ttatcaattt actaggtatc gattcgagtg
    25981 aaagcatcca ggcaatcatt gcggtgacgg gagaagcaga agaaggccac tatctcttct
    26041 ttacgacgcg tcaaggtaca gtaaaacgta cttctgtcac tgcttttgct aacatccgaa
    26101 gtaatggtct gatcgcaatt ggtttgaaag aagacgatga gctcgtcaat gtgctactga
    26161 cagatggtca atctaatatc attattggaa cacataacgg ctactctgtc actttcaaag
    26221 aagaagcagt ccgcgatatg ggacgtacag cttccggggt tagaggaata cgcctgcgtg
    26281 aggaagatta tgtcgttggg gcagccttaa tgaaggaaga tcaagaagta ttagtgatca
    26341 cagaaaaagg ttacggaaaa cggacaaaag tatcggaata tccagtgaaa ggccgcggag
    26401 gtaaagggat caaaacggcg aacatcactg aaaaaaatgg tccactagct ggattgacca
    26461 ctgtttctgg agatgaagac atcatgctga ttacagataa aggtgtcatt atccgtttca
    26521 acgtatcgac tgtttctcaa actggacgtt caacattagg cgtacgtttg atgaagatgg
    26581 aagctgatac gaaagttgtc acgatggcag cagtcgaacc agaagctgca gaacaaacag
    26641 ttaaagaaca aggatcagaa gaatagccat caagactaat tgtgtaagct agcaagaatg
    26701 accgattaaa aaatctggtt attcctgctg gcttttttga ttagttgaat ttctcttaaa
    26761 atattcgaaa ctgttcattc tgtattgctt ttgaccaaaa tagagtgtat aatcagagct
    26821 tgtgagtagt ctaattaatt agataactct ccttgctctt aggaacctaa gagccgaaag
    26881 tccacaggga ggtgaatatc aatggaaaac acgaaatatg aaatcatgta cattattcgt
    26941 ccaaacattg atgaagaagc aaaaactgct ttaatcgaac gtttcgatgc aatcttgaaa
    27001 gataacggag cagaagttat cgaatcaaaa gattgggaaa aacgccgttt cgcttatgaa
    27061 atgaacggtt accgtgaagg tatctatcac atcgttaaag tatcttctcc atcatcagca
    27121 aatgctgtca atgaatttga ccgtcttgct aaaatcaacg acgatatcat tcgtcacatg
    27181 attgttaaag aagaagctta attgttgttt catgtgaaac aatagaaaac gatgagagga
    27241 gatgactatt ttgattaata atgttgtatt agttggacgt ctgacgaaag atccagattt
    27301 gcgttacaca gcaagtggta cggcagtcgc aacatttact ttggctgtga atcgtaattt
    27361 tacaaaccaa agcggcaatc gcgaggctga ttttatcaac tgtgtgattt ggcgcaaatc
    27421 agcagaaact ttagcaaact atgctcgtaa aggaacactt ttaggtgtta ctggacgtat
    27481 ccaaactcgt tcttatgata atcagcaagg acaacgtgta tacgtgacag aagtagtagc
    27541 tgacaacttc caattgctgg aaagtcgttc tgcatctgaa aatcgccaac aaagcggcgg
    27601 attccaaagt tctggtcaat cagctggcgg atttggcgga aacaataact ctaatcaaac
    27661 atctcaatca tccaacggta tgcctgattt cgaccgtgat acttctgacc cattcggctc
    27721 aagttcaacg atcgacattt cagacgatga tttaccattc taaacgaatt tgataggagg
    27781 gaaatagaat ggcacaacaa agaagaggcg gacgtaaacg tcgtaaagta gactacattg
    27841 ctgctaacca tattgaatat atcgattata aagatatcga attactaaaa agatttattt
    27901 cagaacgcgg taaaatcttg ccacgtcgtg taactggtac aggcgctaaa aaccaacgta
    27961 aaattactgt tgcaatcaaa cgcgctcgta tcatgggctt gctacctttc gttagcgaag
    28021 actaataaat cctaggaatt gaatgactga tccttttgat cagctattca attccttttt
    28081 ttctgtaaaa ataacctcta ttcgtttagt cgctcgaatg acgaaagtta tggtaaaatc
    28141 aaattagtca tatatgggag gaaaattatg gaaaataaaa acaatcattt atccccgaaa
    28201 cctctcttcc ttatcctgtt tatattaatc gaagtattgg tagtcttaat tttaccattt
    28261 cgttttgtta gcgcttcggt cattctattg atgaatttat ggggtgttcg ctattggttg
    28321 aaaatgaaac gttggcttat tttaaataaa cgtgaaaaaa tccagcaggc ttcgactgta
    28381 gctgaaacca atctagctta tgtctcagaa gtcatgcctg taggagtgat tgcttatcag
    28441 ccatcaagcc aaaaaatcga atggctcaat ccattagcat tgaaaatcaa agatcaaagt
    28501 ggattaacag aggaagaatt gctagatact ttttttactg caaaagaaaa aaagaaaaac
    28561 caagtctatc tagcagataa aacatttcag tttgatatgg atacagtaaa gggtgttgtt
    28621 tattttaatg atattaccca aaaccagttg ttaaaactcc atcagacgaa cagtcagccg
    28681 gtgattggta ttttatctat tgataactat gcagatgcca ttgataaatt ggatgacaaa
    28741 gagatttctt atttgaactc tttcgtcact acactagttt ctgactggat gaatgaatac
    28801 catgtctttt tcaaaagaat caacgcagaa cggtttttct tcgtagcaca gatgatcgat
    28861 ctgaaaaaga tgatggataa aaaatttgat ttgctggatc gctttcgagc agaagctgaa
    28921 aaacagagct cgcctatcac ccttagtatg gggattgctt atggctcaag aaccctcgat
    28981 gaaatcggtg gagaagctca gaataattta gatgttgctt tagtccgtgg aggcgaccaa
    29041 gttgtcgtga aagaggtaaa agaaggagca aaacctgttt attatggggg gaaatctgct
    29101 aagaatgtca aacgcacgcg tgttcgttct cgagcgatga gtaccgcttt gcgtcggatt
    29161 tttgatgaaa caggcgatgt gtttatcatg gggcacaagt ttccggatat ggatgcttta
    29221 ggctctgctt ttggtgtcag ttgtctggca cgcttcaacc agaaaaaagc ttacatcgtt
    29281 atcaacgaga acgagatcat tccagatgta gaacggtgtc ttgaagaaat ccataagcat
    29341 cctgaattag aaatgcaact aatctctcca gaacgtgcca tagagctgaa aaaagaagag
    29401 gacctcctcg tgatggtgga ttaccataaa ccttctctat cgatctctca gggcctatat
    29461 gagcaatttg ataagattgt gatcattgat caccatcgtc gtggggaaga gtttccaagt
    29521 aaacctctgc tgacttatat cgaatcttca gcttcttcag cttcagagct agtttctgag
    29581 ctgatccaat accaaagcag caaaacaaat cgcttagatc gttttgaggc cacattattg
    29641 cttgcaggga tggttgtaga tacaaatagt ttcaatgtcc gtacaactgc acgaactttt
    29701 gacgttgcca gctatttaag gtcttgtgga gcagacgcat ccttagtaca atatttattg
    29761 agctcggatt tagcgacata cttggagatc agcaatttga ttgctggaag cgactatgtg
    29821 acttctgata ttgttgtggc ggcagggaca gacgaaaaag agtataatag tgttatagca
    29881 gcaaaaacag cagatacatt gctttcgatg atcaatatca atgcagcttt cgtcattaca
    29941 aagcggacag acggcttgat tgggatcagt gccagaagta ccggcacgat caacgtccaa
    30001 atcatcatgg aatccttagg tggcggagga cacttcacaa acgcagctac tcaactggaa
    30061 aatacgacag ttgaggaaac aaaacagaag ctatttgctg caatcaaaga aaatatgaat
    30121 caaatatatg ataaggagta atccaaatga aagtaatttt tttacaagat gtaaaaggaa
    30181 aaggtaaaaa aggggacatc aaagaagttc cagcaggtta tgcacaaaat tatctaatca
    30241 aaaataactt ggcaaaagaa gcaacgaaaa gcagtatcag tcagttagca ggacaaaaga
    30301 aagcacaaga aaaacacgaa gctgaaatct tggaagaagc taaaaagtta aaagaattct
    30361 tggaaaaaga agatactgta gtcgaattga aggcgaaagc aggggaagac agccgtttgt
    30421 ttggttcgat cccttcaaaa caaattgctg aagggttaaa caaacaatac aaaatcaaat
    30481 tagataaacg aaaaatcgaa ttagaaaatc cgatccgttc actaggctac acaaacgtac
    30541 ctgttaagct tcatcatgag gtgacggcta cgatcaaagt tcatgtcgta caagaataac
    30601 aaatcttgat agcgatgttc agctggtaag tataaccaga aggaaaaaag acaaaccgaa
    30661 atttatctga gaaaacagct gcagcaatga aagcaaaagc tgcaaatcga tttcagattg
    30721 atttcggttt cttttttggg aacagcaaaa tagcataaag aaaacgagta taataaaaga
    30781 gaactatttt tttattacac tttcggtacg aatgatgact tttctctcat tttcttgtaa
    30841 aatgaagcaa ttgaagacaa ggagcgatac gtatgaatga agtgtggcag gatcgaatcc
    30901 caccgcaaaa tattgaagct gaacaagcgg ttttaggctc tgtttttctt gacgcagaaa
    30961 cgatcattga tgccatggag tacatagagc caaaagactt ttatcgtcgt ggccatcaaa
    31021 ttatttttga gacaatgatc gagttaaatg atcgtaatga agcgattgac gtggttactg
    31081 tcaaagaccg gctggaacaa gccaatttgt tagaagatgc cggtggttta agttatctct
    31141 cagacttggc attagcagtt ccgacagcag caaatatcgt ctactatgcg aaaatcgttg
    31201 aagaaaaatc attgctgcgt acattgatcc agacagcaac aggaatcgtt acgaaaggat
    31261 tcgagcaagg agaagaagtt caaacaattt tagatgatgc cgaacgcagt atcttggagg
    31321 tttcggaaaa aagaaatcga agcggctttt tatctattgc tgaagtactg aactccacga
    31381 ttgaaaatat cgatcgacta gctcaaaaca atgaagaaat cacaggtcta ccaactggct
    31441 atcaaggtct ggataaaatg acagcaggtt tccagcccga agaactaatc attcttgctg
    31501 cacgtccagc ggtaggtaaa acagcgtttg cattgaatat tgctcaaaat gtaggaacaa
    31561 aaacagatcg ttctgttgcg atatttagtt tggaaatggg ggcagaatct cttgtaaatc
    31621 ggatgctttg tgcagaaggt tcgattgagg ccagccattt acgtacaggt cagctttcgg
    31681 aagaagaatg gtcgaatctg atcgttgcga tggggagtct ttctcgagca aatattttta
    31741 tcgatgatac accagggatc aaaatctctg aaatccgtgc gaaatgtcgg aaactagcgc
    31801 aagaaaaagg caatctaggc ttgatattga tcgattattt gcagttgatt gaaggtaacg
    31861 gtaaagagaa ccgtcaacaa gaagtatcag atatctctcg ccaactgaaa aaactggcga
    31921 aagagctgaa agtacctgtc attgctcttt cacagttatc tcgtggagta gaacagcgac
    31981 aagacaaacg accagtgcta agtgatattc gtgaatccgg atcaattgaa caagatgcgg
    32041 acatcgtagc cttcttgtat cgagatgatt attatgaaag agaaggcgga gatgacgatg
    32101 atggaaacga gccaaaaaat gacaacgtca tcgaagtcat catcgagaag aaccgaagcg
    32161 gtgcacgagg aacagttgaa ctactgttta tcaaagaata caataagttt tcgtcgcttt
    32221 cgccaagaga agaattttaa agaagaggtt gtgagccttg agcaatcccg agagctctcg
    32281 taagaagatg caaggagtaa ccgcaggaac gactagagta cttaatttaa acagatctcc
    32341 aaagggattg tgacaaaagt cttgtcacag tccctttttc cgaataaacg gtgttcaatc
    32401 agctgaccct tggtattagc tcagcaaacg tcaaaatatg aggaactatt ttgacgtttg
    32461 tttctctaat actcaggtca aaacgctgat cttacgacct cttttcctta aacggtgttc
    32521 tcctgccaac tcctcggtca caagtcacaa tatcatcaaa catgagaagc agttttttga
    32581 aattgctcct tgtgactcag agttaaacgg caggctcaca acctctttct taaacggtgt
    32641 tcaaaagctt gcatctgcgg taatacgccc aatcaaacga aaaatatgag aagctatttt
    32701 tgtttggctg ttcttattac ttgatgcagg acagcttttt cacaacctct gattcacggt
    32761 gttccaaaag tagcttcttc gaaaaataag tcgaaatttc acaaaaattt gagaagcaat
    32821 tttcgtacat tccttcttat ttcttgaagc tgaccacttc tgttacaacc tcttttttaa
    32881 taaccattct aaaaagataa agttcgtttt ttaagcgttt ttataaaata aaaataaaaa
    32941 tcattcgatt ttatttgaaa tatgtatcta ttattgatac aatggttcag gattaaataa
    33001 tatagaacga ggtgttcaca tgtcatctgt agtagtagta ggtacgcaat ggggcgatga
    33061 agggaaagga aaaatcacag actttctcag tgaaaatgct gaagtgattg cacggtacca
    33121 aggtggcgac aatgcagggc acacaattaa gtttaacggt gtgacttata aactgcattt
    33181 gattccttct gggatttttt ataaagacaa gatcagtgtg atcggaaacg gcgttgtagt
    33241 caatcctaaa tcattggtta aagaattagc ttatttacat gaaaaaggtg tagaaacaag
    33301 taatttgcgt atttctgacc gcgcgcacgt aatcttgcct tatcatatca aattggacca
    33361 attacaagaa gatgctaagg gcgaaaataa gattggtaca acgattaaag gaatcggacc
    33421 tgcctatatg gataaagcag ctcgtgtagg tatccgtatt gcagacttat tagataaaga
    33481 aatttttgaa gaacgtcttc gtttgaatct agatgacaaa aaccgtcaat ttgtaaaaat
    33541 gtttgattca gaaccacttg attttgacga tatttttgaa gaatattatg aatacggaca
    33601 acaaatcaaa caatacgtca cagatacttc cgttatcttg aatgatgctt tggatgcagg
    33661 caaacgcgtg ttgtttgaag gcgcacaagg tgtaatgctg gatatcgacc agggaactta
    33721 tccttttgtt acttcttcta acccagtagc cggtggggta acaatcggaa gcggtgtagg
    33781 gccttcaaaa atcaataaag ttgtcggcgt ctgcaaagcc tatacgtcac gtgtcggtga
    33841 tggaccattt ccaactgaat tatttgatga aacaggagaa caaatccgcg aagtaggacg
    33901 agaatacggt acgacaactg gtcgtccgcg tcgtgtaggc tggtttgatt ctgtagttat
    33961 gcgccattct aaacgagttt caggtattac caacctatct ttaaactcga tcgatgtatt
    34021 aagtggattg gaaacagtaa aaatttgtac ggcttatgaa ttagatggcg aactaatcta
    34081 ccactatcca gcaagcttga aagagttgaa ccgctgcaaa ccaatctatg aagaattgcc
    34141 aggctggtca gaagacatta caggatgcaa aacacttgca gaactgccag aaaacgcacg
    34201 taactatgta cgtcgtatct cagagcttgt tggtgtacgt atctcaacat tttctgtcgg
    34261 acctgataga acacaaacaa acatcttaga aagcgtctgg gcgcaaatct aaggataaaa
    34321 acgacgactg aatcaggacg ttcttaccag agcgagtcga agtgtttatc tatacgataa
    34381 taataacttg aacactgttt attcagaata agggactgtg acaaaaa
//
LOCUS       DACGLE010000024        32571 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0534
            SAMEA3304449-rid9560453.denovo.025, whole genome shotgun sequence.
ACCESSION   DACGLE010000024 DACGLE010000000
VERSION     DACGLE010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304449
            Sequence Read Archive: ERR985064
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 32571)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32571)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702225.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702225.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 20:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,045
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 27 of 67
            Pseudo Genes (incomplete)         :: 25 of 67
            Pseudo Genes (internal stop)      :: 19 of 67
            Pseudo Genes (multiple problems)  :: 4 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32571
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304449-rid9560453.denovo.025"
                     /strain="VREN0534"
                     /db_xref="taxon:1352"
     gene            <1..189
                     /locus_tag="IXT20_001258"
     CDS             <1..189
                     /locus_tag="IXT20_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002419316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAQ4859714.1"
                     /translation="NSNGILRRNGLPKSIDFREVNQTFISSVSNQRNHIPRKSLNYRT
                     PIEIFLSYVQEAFYSSLI"
     gene            complement(1055..1585)
                     /locus_tag="IXT20_001259"
     CDS             complement(1055..1585)
                     /locus_tag="IXT20_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transferase"
                     /protein_id="HAQ4859715.1"
                     /translation="MRLGLIIKNILNLPKTIYFNYKFLHISDAWRLPILVHRKVRITR
                     KSRGKIEIVLQSGKIVYLGFPVLNTAGNEETIISIEGILRIQGPAFIAQGAHIDIRKN
                     AVLDIGSKFNSTGKMSIICFKEIQFKDGCTVSWDTLFMDTDSHTIFELNTHRILNKNN
                     KIVIGKHCWIGAKSVI"
     gene            complement(1582..2199)
                     /locus_tag="IXT20_001260"
     CDS             complement(1582..2199)
                     /locus_tag="IXT20_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HAQ4859716.1"
                     /translation="MIIVIAEIITDILYKLYEPFGFAVLLTILGLVYFMYATDGIAAG
                     EGYKRILMELYLHLKKDRKYRKVCALIFYTAMILFTTLINRTIWLNPLSDVMGDWWIY
                     KYNTTTGTKELSTACLENIILFIPYVYMVMSIKYENIKKPKIWSSIVYGGKIAFYFSA
                     IIEFLQLFLRLGTFQISDFVYNTLGGILGVILYKLILITKRDGER"
     gene            complement(2781..3002)
                     /locus_tag="IXT20_001261"
     CDS             complement(2781..3002)
                     /locus_tag="IXT20_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transferase"
                     /protein_id="HAQ4859717.1"
                     /translation="MGSSERIKIGNNVFIGMKSRILKGVHIGNNVVIGANSLVNKDVS
                     DDCVVAGNLAKVIMSLDEYYKKINNMKNP"
     gene            complement(3180..4712)
                     /locus_tag="IXT20_001262"
     CDS             complement(3180..4712)
                     /locus_tag="IXT20_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAQ4859718.1"
                     /translation="MIINRKQNAKRNIITGTIFKLYQIIIPFFMRTLMIYQMGMEYAG
                     LNNLFTSIFQMLNLAELGIGAALTVSMYKPIAEDNRKEICALLNAYKKYFSIIGWIVF
                     TLGLLCIPFLRFLVKGSIPGNLNMTVLYLMYLINTVLSYWLFSYRKSLIYAHQRNDVI
                     NVSMLITFTFQFVMQAYVLIFLKNYYLYLLASILGQIVLNLMCAVVSQKMYPDYTPVG
                     ELEKETIDDIIKKVKGLVTNKVGGTILRSSDSIIISSFLGLSVLAIYQNYYFIISAIM
                     SIFAIIYESCIAGIGNSLIVESQEKNYIDFCDITTIIGWFLAISCACFITLFQPFMII
                     WVGSDNLMSISFVIFMIVHFFLYEIDQLIGLYKDAAGIWYADRFRPLITAMINIVLNI
                     ILVNWFGLYGVLAATIVSVLLVDLPWLLYNVFQNIFKFESAGKYVWKLISLCVIAFVA
                     SYLSYFACSYVNIGNFKVISLVINLVISVVISILVILLFNIRNKKFKYILKVLTSVLV
                     KR"
     gene            complement(4848..5933)
                     /locus_tag="IXT20_001263"
     CDS             complement(4848..5933)
                     /locus_tag="IXT20_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HAQ4859719.1"
                     /translation="MKKICFISGVISRSGGTERVGSIIANSLSDLGYDVTILSFWNSG
                     EPYFGLDKTIKVDYMLNPKTEGKLYRTYLYPVWKLHKYICKNEFDIVIDIDTLLSLYT
                     TRAIKGTKCKLISWEHFNYWTMEMLKEKKRFKAKKLIKKYAKRLVVLTEEDRQKHIDE
                     YSLSEDFVITMPNPCISNIEYEYHYDKKRFLAVGRLVEEKNFADMISAWKIVEKKVDD
                     WDLIIVGKGELEKDLRQQIKDLNLQRVTIAGHSDHVEEYYKSASCYVLSSVYEGFPMV
                     ILEAQSYGLPVISYDCKTGPRDLVHHNFNGMLVEDKNIDQLAKSMIMFTKNTDLAMRM
                     SLNAYNNVQKFNLKEITKQWVALIENV"
     gene            complement(5948..6667)
                     /locus_tag="IXT20_001264"
     CDS             complement(5948..6667)
                     /locus_tag="IXT20_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HAQ4859720.1"
                     /translation="MIPKKIHYCWFGGNPLPKKDRKCIESWKKYCPDYEIIEWNESNY
                     DLNKNRYMADAYKERKWGFVPDYARFDIIYNHGGFYLDTDVELIKSLDILRENHAYMG
                     FEGEIWVNGGIGFGAEEGHSVIKGLRDIYDSLSFYQNDGTLNLKPSPYYITDYLESIG
                     LVRDNSEQIINGLHIYPTDYFAAKDYDTGKIYKTSNTISIHQYNASWKTPKQKFVYRL
                     KSTIGQKKFNRLLALKKFLHL"
     gene            complement(6664..7974)
                     /locus_tag="IXT20_001265"
     CDS             complement(6664..7974)
                     /locus_tag="IXT20_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HAQ4859721.1"
                     /translation="MRISIKRWSGADWTLFTLAFFAISIVLVYTFNAPRAILYLGDII
                     NIFVLLNALSFRRRKGIYASKNEMLIVLFLLVSICSSVINLENIGLIIWGIRNNTRMF
                     IVYINCATYLTIKDYYKVFAIIEKLFWISLPLCLIERFMVTYPTGTIVGDMIGGVFWN
                     YSGCNMPLNLILCVTVTVVTIGYFKGEKNFIHFILCVCAALFMSALAELKVFIPELFI
                     IFVAIVFTLKVDFKVVIKILSCVILVSIVLSFFITIFITLNDNGQSYADNFTIQGLLE
                     YASRDSGYDGVGDLNRITGISRVVDEIFNNNFRLVLMGVGIGNAEFTNFFVSEFYKSF
                     GNLHYQYFHHIWLFIEVGILGVSIFLFFIIKTFFNAKRSFMKDKDASLVKVIAIMSVF
                     FFVYNTTLRGETTGPLIYMILAMPEVVKRKEHYYSEKNTKGEIL"
     gene            complement(8001..8591)
                     /locus_tag="IXT20_001266"
     CDS             complement(8001..8591)
                     /locus_tag="IXT20_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-gamma-glutamate biosynthesis protein"
                     /protein_id="HAQ4859722.1"
                     /translation="MEISSKEYLKAVLRMEKNLYIESKGQHTKLLKIMKSHPDYNAWR
                     YTRRMRITSYYYSKRKKNIIYAIMYIVSCRRMNSLGRKCGIECGENVFDEGVRFYHTQ
                     GIVINGNAIIGKNCYLYGNNCIGNDGIDPKCPVIGNNVRVCVGAKIIGNVKIANNVVV
                     AAGAIVIKDCLENGAILAGVPAKIIGYVNLTKVFLE"
     gene            complement(8909..9970)
                     /locus_tag="IXT20_001267"
     CDS             complement(8909..9970)
                     /locus_tag="IXT20_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAQ4859723.1"
                     /translation="MNSSDLISVIIPVYNVEKYVGSCLESVCNQTYKNLDIIIVDDGS
                     KDSSNKICEDYALKDSRIRIIHKNNAGLGLARNTGLEHVKGKYVAFIDSDDFVAANYI
                     EAMLNGIKRSNADTCYAGYYEYYDDNNIISKPARYDSTIFTNNAIIEKVLLGMIGTEP
                     SYKVDFILPMSVWHALYSSEIIQQHNIKFPSERDYISEDIIFHISYLQHSKITSFIKD
                     CIYYYRCNNNNSLTHNFRDNEFDCNIELVKKIEGDLSYILENEIFVNKTRRLLLGRCR
                     TCIQKAVRYASFNKEFDLNSYLSKILMNDELIACIHSYPYKQNPIKQRIFNYFLLNQS
                     IIGVKTLAYLSGMGRNRRF"
     gene            complement(10538..11605)
                     /locus_tag="IXT20_001268"
     CDS             complement(10538..11605)
                     /locus_tag="IXT20_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HAQ4859724.1"
                     /translation="MQRVLMIGNDSSVKGGITSIITQMLNFNWEKKNIEMTFIPSYKG
                     GNAIFKSLYFMYAFAKIIGISLLSKPNIIHMHMSYRGSFSRKYVLNKWFRLLNIPVII
                     HLHGSEFKKWYDSVSVKKQNQIRLLLRESDSTIVLGEKWKKIIKEIEPNCKTVVVENS
                     IVVPEYTIKWNSKMCRFLFMGVLIKRKGVHDLVEAVKNIAEQYPEKKFIFEIAGAGDE
                     EISLKELIEEYNLQSYFEFTGWVVGEKKEECYRRSNVMILPSYNEGLPISILEAISYG
                     MPVIATDVGDISSAVRDGENGYLFQPGDVSALTSAILKINDKDKYVKFSRCSKDLAIT
                     KFNDGNFFDRILNLYQKGSKE"
     gene            complement(11625..12104)
                     /locus_tag="IXT20_001269"
     CDS             complement(11625..12104)
                     /locus_tag="IXT20_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta(1,3)galactosyltransferase EpsH"
                     /protein_id="HAQ4859725.1"
                     /translation="MIFITLGSQKFQFDRLLKAIDDLIEKGVIIESTFAQSGYSDYKP
                     LNYSYKAFLNRDEFTETMQKAEMVITHGGTGAIIGAVKKGKRVIAVPRLAKYGEHVDD
                     HQLQLIRQFDEMNIIYPCYELENLEDAVKETRVRMFNKYVSNTETIINSIEVLIEKI"
     gene            complement(12121..12582)
                     /locus_tag="IXT20_001270"
     CDS             complement(12121..12582)
                     /locus_tag="IXT20_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAQ4859726.1"
                     /translation="MNKKICFAASSGGHYEQLMMLKPLMEKYESFVITEQTEYSAESK
                     GEKTYYMRQVNRKEKFFLCTILINLFKSIIIYIKEKPDLVICTGVLAMIPICLITKMM
                     GKKLIYIESFAKVTSPTQTGKLMYHFADQFYVQWETMLQFYPNAIYLGGIY"
     gene            complement(12596..13846)
                     /locus_tag="IXT20_001271"
     CDS             complement(12596..13846)
                     /locus_tag="IXT20_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HAQ4859727.1"
                     /translation="MKKKYNIAVAGTGYVGLSIATLLAQHHQVTAVDIIPEKIELINK
                     RKSPIQDEYIEKYLTEKELDLTATLDARSAYKSADFVVIATPTNYDSRTQHFDTSAVE
                     SVIEVVMDVNPDTIMVIKSTIPVGYTESVRETTGSKNIIFSPEFLRESKALYDNLYPS
                     RIIVGTDMTDERLLNAAHVFATLLQKSAIKENVDTLFMGFTEAEAVKLFANTYLALRV
                     SYFNELDTYAEMKGLNTQQIINGVCLDPRIGTHYNNPSFGYGGYCLPKDTKQLLANYA
                     DVPGNLIEAIVESNRTRKDFIADRVLQMAGYYNYAEYNQYDVNRENPVLIGIYRLTMK
                     SNSDNFRQSSIQGVMKRVKAKGATVVIYEPALKDGETFFGSKVINDLEQFKAMCDSII
                     ANRYDTCLDDIKEKVYTRDIFQRD"
     gene            complement(14224..15675)
                     /locus_tag="IXT20_001272"
     CDS             complement(14224..15675)
                     /locus_tag="IXT20_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004612957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HAQ4859728.1"
                     /translation="MYKKSSRGWVKHIDFIILDLLCIQLAFYLSYVLRQVDWNPYVIP
                     MYQNMAVFIELADLLVIFMFEAYRGVLKRGYYIEFASSVKQAIMLVLLGSLYLISVQG
                     GNDYSRVVLFLMGGLYVVITYITRLLWKHYLKHKMKNEGNKSLIIVTSSSIAEVVIRN
                     VKEHNYKMYKINGVIIIDKSMIGEDVEGVTVVSDSKNAADYLCQGWVDEVFIILNKST
                     SCMKEFIERCSEMGLTVHLNLAKVSDSALGKQSIGRIGEYTVLTTSINVMTTQQAFIK
                     RTIDILAGIVGCIATGIIFIFIAPLIYINSPGPIFFAQERIGQNGKPFKMYKFRSMYM
                     DAEERKVELMKENKMSNNLMFKMEFDPRIIGNKILPDGTKKTGIGQFIRSTSLDEFPQ
                     FFNVLTGKMSLIGFRPCLKSEYEEYDYHHRARIAIKPGITGMWQVSGRSDITDFEEVA
                     KLDTEYIKNWSMGLDFKILFKTVKTVLQRGGSM"
     gene            complement(15996..16760)
                     /locus_tag="IXT20_001273"
     CDS             complement(15996..16760)
                     /locus_tag="IXT20_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein phosphatase"
                     /protein_id="HAQ4859729.1"
                     /translation="MIDLHCHILPGIDDGAENLEASIAMAEKAIQQGITHILCTPHHN
                     NGKYSNEKSQVISLVASLQAELEKRQLPLTLLEGQEVRITGTLIEDIHRDEILFTDLD
                     DTYLLIEFPTLEVPLYAERLFFELLELGKTPVIVHPERNVHFREDPNHLLPFLDMGCL
                     AQLTAPSYVGVFGKDIQKTAKTMVKHNLVQMAASDAHGVSKRTFYLKECYEQIAKDFG
                     KEKVVQMKQVAKDLINGDEISYPVYEEVKKKKFGLF"
     gene            complement(16788..17486)
                     /locus_tag="IXT20_001274"
     CDS             complement(16788..17486)
                     /locus_tag="IXT20_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpsD/CapB family tyrosine-protein kinase"
                     /protein_id="HAQ4859730.1"
                     /translation="MARTQKQKAAQTHAVSLITLTNPSSPIAEQYRTIRTNIQFASAA
                     GQQIKTIVVTSSGPGEGKSTTAANIAVVFAKSGQRVLLVDADLRKPVVYKTFQLNNAS
                     GLSTALSSSGSVADVIQRTPVENLSILPSGPKPPNPSELLSSPRMDQILSEARQLFDV
                     VIFDMPPVVAVTDAQIMSLKTDGTLLVVRENMSRKESLTKAKELLEMVQARVLGVVYN
                     GAEHSKDAGYYYGN"
     gene            complement(17498..18277)
                     /locus_tag="IXT20_001275"
     CDS             complement(17498..18277)
                     /locus_tag="IXT20_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein kinase"
                     /protein_id="HAQ4859731.1"
                     /translation="MEETISLKEIFETLRKHLVIIIISMFAGLAISGIVTFFILTPKY
                     SSQAQLIVTLPQSDTANVNDVNTNLQMINTYKDMIVSDLVLNEVKDRLETEDNVKMTA
                     EQIKDAISVNQAENSQMFSIQAISTNAVAAQQIANTTSQVFQENAKDVMNVDKISIIS
                     GAVANTTPVSPNNKLNLMTGLMLGMMVGVGLAFLLELLDRTVKDEKYVTDTLGFPILG
                     TVPEMSAKELNAGIRKKGTAPARPKATNDKTAPSRRSRSRV"
     gene            complement(18293..19237)
                     /locus_tag="IXT20_001276"
     CDS             complement(18293..19237)
                     /locus_tag="IXT20_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAQ4859732.1"
                     /translation="MARRRRKSRGKKIFLIILAVLIVLVLAIVGVGAKLYLDLSHSIE
                     KTYESVERTQGERTKPVDLDKQESFSVLLMGIDTGDLGRVDQGRSDTMMVATVSPEDK
                     QTTIVSIPRDTYVDIVGRDTKDKINHAYAFGGAAMAMDTVQKYVDIPIDHYVSINMKG
                     LKELVDAVGGIEVNNDLTFSQDGYDFPIGKITLNGDQALSYSRMRYEDPNGDYGRQGR
                     QRKIVEGVAKKVLSLNGVSNYQSVLNALESNMKTDMNFDEMKKIALDYREAFGTIKQD
                     QLQGEGFMQDNISYQKVSDQELDRVKKELKDQMNLENK"
     gene            complement(19283..20062)
                     /locus_tag="IXT20_001277"
     CDS             complement(19283..20062)
                     /locus_tag="IXT20_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4859733.1"
                     /translation="MEKPFVKLLATVAVGTGAIVICLFGYHLNNQRQYHQRINYAESA
                     ITNQKDTVTSLSKEVDKLYSTKEKIFLNPEITEETVSNLSHKLSSIKLSADDYDIKES
                     ELPKEAAAIQEEKKAVLTQLEDAESKLKIQTAVNKLFTKNVSNWQQAVDDVIIKEKLA
                     SADVAHVRENMSFFKDSAWKTVVMQYLGFADTQIAQVTQLDQLFDTMLKDGQVTATAT
                     YDQYLTALSQIEQIRNEKIRAAYATKAQTVAQQMGYSNISY"
     gene            complement(20442..22517)
                     /locus_tag="IXT20_001278"
     CDS             complement(20442..22517)
                     /locus_tag="IXT20_001278"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HAQ4859734.1"
                     /translation="MAKDLLLEIGLEEMPAHVVTPSMKQLEEKTAKFLDENQLTYDTI
                     ETFSTPRRLAIKVTNIPERQADSEEEVKGPAKKIALDADGNWTKAAEGFVRGQGLTTD
                     AITFRELKGVEYVYVTKYTHGKEAKEVLTGLAGVIKSLTFPVTMHWAEYDFEYIRPIH
                     WIVALLDDEVIPFEILDVKTGRASRGHRFLGEDVTFAHAKEYEEKLNEQFVIASPQKR
                     QEMIVTQANELAEKNHWVIDLDEDLLEEVNNLVEYPTVFSGDFEEKYLSVPEEVLVTS
                     MKEHQRYFEVRNDQGMLLPHFISARNGDRVKLEDVVRGNQKVLTARLEDAEFFYNEDK
                     KASIDQYVEKLKTVTFHEKIGSIYEKMQRVQVIARIIGEQVGLSEEELMDLQRAAEIY
                     KFDLVTNMVGEFPELQGIMGEKYALLKGEKPAVATAIREHYMPISSEGELPQSTVGSV
                     LAIADKLDSIFSFFAVGMIPSGSNDPYALRRQAYGVVRIIESKKWAFPLSVLQETISD
                     VISKDTDRFGIGLSEGQQQVIDFIKGRLRQLLTTKNIRHDVIDAVLNAEQKDLTKVFA
                     AAQLFKQHLADEDFKPSMEALTRVVNLAKKAQLEKQSEVDPELFENDAEKELHKAVEA
                     MKASFADQTIHENYDQLVALRPLIENYFDQTMVMADEPAVRKNRLTQLSQIAAMALSL
                     ASLDQIITK"
     gene            complement(22519..23436)
                     /gene="glyQ"
                     /locus_tag="IXT20_001279"
     CDS             complement(22519..23436)
                     /gene="glyQ"
                     /locus_tag="IXT20_001279"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HAQ4859735.1"
                     /translation="MSKKLTVQDMILTLQKFWSSNGCMLMQAYDTEKGAGTMSPYTFL
                     RAIGPEPWNAAYVEPSRRPADGRYGENPNRLYQHHQFQVVMKPSPENIQELYLESLRL
                     LGIDPLEHDIRFVEDNWENPSMGCAGVGWEVWLDGMEITQFTYFQQVGGLPCKPVTSE
                     ITYGLERLASYIQEVESVYDLEWADGVKYGEIFKQPEYEHSKYSFEVSDQDLLLSNFD
                     RFEKEAKRCIDENLVHPAYDYILKCSHTFNLLDARGAVSVTERAGYLSRIRNMARAVA
                     KIFVAEREKLGFPLLNKNTANPETVKEAE"
     gene            complement(23822..24640)
                     /gene="recO"
                     /locus_tag="IXT20_001280"
     CDS             complement(23822..24640)
                     /gene="recO"
                     /locus_tag="IXT20_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HAQ4859736.1"
                     /translation="MSQLAESKGLILSSRNYKEKDKLVKIFTESSGKMMFYIKEIHRQ
                     NQPLAPALLPFTEAVYIGNFRSEGLSFLSSVKDVHAFRHIQEDIFLSGYGTYLLNLVD
                     AAIEDHQYDPHLFQFTQQALQRMDQGDDAEIITNIFEVQILQRFGIAPIWTHCVVCGE
                     TKGKFDYSSKYGGVICERHWSMDDHRYHADPRAVYFVRMFSAISYDKISGIHLKEETK
                     QAIRELIDQLYDEYVGIHLKSKKFINQMKSWAHVLKTSEKDMSEKEKETPEKEE"
     gene            complement(24783..25682)
                     /locus_tag="IXT20_001281"
     CDS             complement(24783..25682)
                     /locus_tag="IXT20_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HAQ4859737.1"
                     /translation="MPEHKSGFVAIVGRPNVGKSTLLNRIVGQKIAIMSDKAQTTRNK
                     IQGVYTTPEAQLIFIDTPGIHKPKHRLGDFMVEAAYSALKEVDAVLFMISADQKRGRG
                     DDFIIERLKNVHSPVYLIINKIDKIHPDELLGIIEDYSTQMPFAQVIPISATEGNNVE
                     RLMDVLVSEMPEGPQYFPDDQVTDHPEYFIVSELIREKVLFLTRDEVPHSVAVVVDSM
                     KRNENDKIHIQATIIVERDSQKGIIIGKGGKMLKQVGTKARKDIENLLGDKVFLELWV
                     KVQKDWRDKKVYLQDFGYRKDEY"
     gene            complement(25697..26098)
                     /locus_tag="IXT20_001282"
     CDS             complement(25697..26098)
                     /locus_tag="IXT20_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HAQ4859738.1"
                     /translation="MPMDLKDKNVEKNKHFLMSVEFAVQGVKTVFEEERNMRKHVAFG
                     ILALFMGVIFQLDRIEWLWLLLAVFLVWIVEIINTVFENVVDMFTDFHFHPIGKKIKD
                     MAAGAVLVTACFTVIVGLILFVPKIYHLLFN"
     gene            complement(26076..26552)
                     /gene="ybeY"
                     /locus_tag="IXT20_001283"
     CDS             complement(26076..26552)
                     /gene="ybeY"
                     /locus_tag="IXT20_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HAQ4859739.1"
                     /translation="MDITFMDETNQVSEEKMKEIDDLLQFAASYLELPEETEMSVTFM
                     DNAAIQVINRDYRGKDAPTDVISFALEEEGEDEIPVIFEDGENPLPRVLGDIMISTER
                     AAEQAEAYGHSYDRELGFLAVHGFLHINGYDHMTPEDEKEMFGLQKEILDAYGLKR"
     gene            complement(26581..28773)
                     /locus_tag="IXT20_001284"
     CDS             complement(26581..28773)
                     /locus_tag="IXT20_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HAQ4859740.1"
                     /translation="MLNKTLQKLQEKLGKAALPFLLFLFFIIVCGVTFSSVKQKAIDF
                     REGQVAEESIRANKTIENTEETEQRRKLAAEAVTPEYTYQKDLADDQNNRIKQLFELI
                     DKTNDTINKSYNGKVDKAKEDENIPKPTTEERIATLKKNFENVNAENVAFYQKLPNNF
                     YSTIFEMTETEIHTVRDESLKLIDEQMSKQVRESELEAFKQEAEDQIQYLNVTTEQQQ
                     MIRYLVDQGIVVNDVLNEKKTEELKQSAREAVQPVMIFQGEIIVREGNQIDASAMKKL
                     ELLGLTNQTTSIFPLIAMVLAVLLQIGVLIYNSLQFQNAGERTKYVLFYVTAMSVSVV
                     LMKFFQLFQTEQAAYIPLFYPAAFAPLVLSFFLNRRAGIMAGIFQAVSALFIFYDSIG
                     TNFLTVILMSYVFSGLMATIVRRKRISEQGLSAAMWVIIFPVMMDVILIIYQGMSFGD
                     ATTWLTLVCGFTGALFSFLLTIGLHPYIELMVNDDSVIVLNELSNPNHPLLKQLLEEA
                     PGTYHHSMMVANLSANAVAEIGGRSLLTRVACYYHDIGKIKHASFFVENLPSGAENPH
                     NFLLPEDSKHIIFGHVTDGAKILEDYNMPQMVIDICRQHHGTTLMRYFYVKAKERNPE
                     VTEEQFRYPGPKPQSREAGIVNIADSCEAAVRAMDHPTSEKIESFVHNLIEERISDGQ
                     LDDSGLTLKEIRKVEKSLISGLSSTFHSRIKYPKMKSEVEKIKEEQEE"
     gene            complement(28797..29768)
                     /locus_tag="IXT20_001285"
     CDS             complement(28797..29768)
                     /locus_tag="IXT20_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HAQ4859741.1"
                     /translation="MTEESSSLEIRLTAADDVSMLLGAHDKHIKLIEETTETTIHTRG
                     EIIQIIGEQSQISLAASVIRTLQELIKRGIHVSTPDVVTALKMGQKGTLDYFVEMYEE
                     EIVKDRNGKPIRVKNSGQKKYVESVAKHDIVFGVGPAGTGKTFLAVVLAIAALKKGEV
                     QKIILTRPAVEAGENLGFLPGDLKEKVDPYLRPVYDALYQIFGLDHTNRLMERGVIEI
                     APLAYMRGRTLDDAFVILDEAQNTTVAQMKMFLTRLGYQSKMIVNGDTSQIDLPRGTT
                     SGLVHAERTLKQIKKIDFVNFEASDVVRHPVVAEIIKAYEKADLHQE"
     gene            30473..31852
                     /gene="brnQ"
                     /locus_tag="IXT20_001286"
     CDS             30473..31852
                     /gene="brnQ"
                     /locus_tag="IXT20_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HAQ4859742.1"
                     /translation="MEETKTYSWKQLLMVSSLIFGMFFGSGNLIFPVHLGQMAGANWV
                     SAGVGFAISGALFPLLAILAVVVTKSDGLYDLAKPVGKWYAALFLILVHLTIGPFFGT
                     PRTAATAFEMAAKPFLPEKYNSLGMFLFSALFFLLAYFLTVHPTRLVKYVGKYLNSLF
                     LALLAIIFVVSLFHPMGNLNQSAADTYQTNALTNGVLEGYNTVDAVALLALSVTFVHA
                     VRDLGFKDKKVTELTAKAGSLSVALEVMIYFGLVLLGAMSLNKMSLSENGGTALSQIT
                     NFYLGNFGASFLGVMVTLGVFTTAMGLVVSFAQDFHKLFPKVSYMTWLRLTTFVSFVV
                     ANAGLDNIIQWSLPVLMLLYPLSLALILLSLTAKFFQKTPFVYQVTMLFAAVPAVLDM
                     LANSPALVSQQRVVASMLEFYHHHVPFAALGLGWMVPTLLGYAGSLLFYYAYRLSGYK
                     QEANELPEE"
     gene            32134..>32571
                     /locus_tag="IXT20_001287"
     CDS             32134..>32571
                     /locus_tag="IXT20_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAQ4859743.1"
                     /translation="MSYSEFTKELLDILDLNLIFHEDAFRKERINDETCFVFDGTLTY
                     QPEECFHCHYQNKQTIIKWGWKKVSILLNDVSNYKTILRINKQRFKCKHCGKTFLAED
                     SVSDRRCSIARRVKQAILELLSEPLSMSLIARMKHISPTTVIRI"
BASE COUNT         9788 a         6654 c         4870 g        11259 t
ORIGIN      
        1 aattctaacg ggattctgcg tcgtaatgga ctgccgaaat caatagattt tagagaagtg
       61 aatcagacat ttatttccag tgtcagcaat caacgcaatc atattccaag aaaatcattg
      121 aattacagaa caccaatcga aatatttttg agctatgtac aagaagcatt ttattctagc
      181 ttaatttgac aaatcatata atttaaaatt aacattgatt ctcctatgaa aatatctttt
      241 acctttctcc tgtgaaaagg aaatttcctt ttttatagtg ttattgatat aatcagaata
      301 ttcatcaaac tctgtatctg gagttaaaaa tctacagtaa catctataag ctttgctagt
      361 aatcttcttg ggttctctct ccattgtcaa gcaacatcaa aacacctact tactattcga
      421 ttcttttctg ccaaaaacga gtcagcatca ctcacaactt atgtgttgct gactccgact
      481 agtcctttgt tcaatatcaa cttattaaac agatataata tttttcttta tttcgtatac
      541 tccaagactt ccatctttct ttcatcttat ctctgaaaaa aacacagcaa agtgccctta
      601 ataaatatac attagaattt gcaaattttt tcatcttaag atatatccta ttcatattct
      661 ttacaatcct aatgggtgat tacaaatcta tttagttcaa gttctacatt ataaacacct
      721 ttcttgatag tatcatctgc tgcacaagcg atgtcgatcc acttattagg ggatttacgt
      781 gctcaaaagt ggctattcct tttgggaagt ccatccataa taatggtcca tggatttttt
      841 tagggtcttg tcgttcaaat ttctcaaaaa ctttattata cttccatagt tttataatta
      901 tttggtttca ctttggctcg ttcaaacacc aacttgtatt tcttttcaac gttcgaactg
      961 gagccccccc tataatctca ttattatttt catgactttt tactaccgtg gaattacaag
     1021 caacaacaat attatctgct aaatgaacac ctttctatat cacgcttttt gctccaatcc
     1081 aacaatgttt acctataact attttattat ttttattcaa aatcctatga gtattcaatt
     1141 caaaaatcgt atgtgaatcg gtatccataa ataatgtatc ccacgatact gtacagccat
     1201 ctttgaattg aatttcctta aagcaaatta tactcatttt tcctgtcgaa ttaaactttg
     1261 acccaatatc taaaactgca tttttcctta tgtcaatatg agccccttgt gcgataaacg
     1321 ctggcccttg tattcttagt attccttcaa tacttataat cgtttcttca tttcccgcag
     1381 tatttaatac tggaaagccc aaataaacta tttttccaga ttgtaatact atctcaattt
     1441 tccctctcga tttgcgtgta atccgtactt tacgatgcac caaaataggt aatctccacg
     1501 catctgaaat atgaagaaat ttataattga agtatatcgt tttaggtaaa tttaatatat
     1561 tctttattat caatccgagt ctcatctttc accatctctt tttgtaatca aaattagttt
     1621 ataaaggatt acacccaata taccgcccaa cgtattatat acaaaatccg aaatttgaaa
     1681 cgttcctagc ctcaaaaaaa gttgtaaaaa ttcaattatt gctgaaaaat aaaatgcaat
     1741 ttttcctcca tatactattg aggaccatat tttgggtttc tttatatttt catatttaat
     1801 agacattacc atataaacat atggaataaa aagaataata ttttctaaac acgctgtaga
     1861 taactctttt gttccagttg ttgtattata cttataaatc caccagtctc ccataacatc
     1921 agataaagga tttaaccaaa ttgttcgatt gattaatgtt gtaaataaaa tcatcgcagt
     1981 atagaatatc aaagcacaaa cttttctata ttttctatcc ttctttaaat gaagataaag
     2041 ttccattagt atacgtttat agccctcacc tgcagcaatg ccatctgttg catacataaa
     2101 atagaccagt cctaaaattg tcaataaaac agcaaaccca aagggttcat ataatttata
     2161 taatatatct gtaataattt ctgcaatcac tattatcata attatttctc caatatcaag
     2221 tatacactta tcgcttccaa ttacacatga tcatattaga gtgtattatc acattctcta
     2281 atattcaatt tatttttcct aaaatattcg aaataatcgt cttgataacg agtaagtatt
     2341 tttatacgat tgataacata gtaacaatat ttttaattat agtaaaaaat catctgcctt
     2401 tttcatccac ctaatcttat ttttcatttt attcaacatc cttaaataaa cttccgttcc
     2461 taagctctat aatatcttct taaaaaataa tattatggtg tcgttttcaa actcgtaatc
     2521 ttttacattg gaaaaataca tacttttctc aatgtagcaa ctcctatctc acagtattca
     2581 aaaattcttc aatattatga aatacttgtg catgttcttt aaataccttg tcagtctgtt
     2641 tataatcccc tactaaattt tgcattgctc ccaactcttc gacaaattgt catctccatc
     2701 tgtaaacagc cctaaaaact catataatgc tttttcatca atatttttcc catattattc
     2761 tcggtatagt ttcacaagtt ttacggattc ttcatattgt ttatcttttt ataatattca
     2821 tctagggaca taattacttt tgctagattt cctgcaacaa cacaatcgtc tgatacgtcc
     2881 ttgtttacta aactatttgc acctataact acattatttc ctatatgtac accctttagt
     2941 attctagact tcattcctat aaaaacatta ttacctattt tgatcctctc cgatgatccc
     3001 aaagcttctc catactactt ttaaaaccga tcaatcataa acatgagtca aaattgtaac
     3061 tccttcatat catctccaat ttcaacaaga gtcttcgttg gaacaaaaac tatacatcgt
     3121 tcgcctattc ggcagcctaa aaattgtagt tactttatat atcattctga tgtctcttct
     3181 catcttttta ccaataccga cgtaagaact ttcaaaatat acttaaactt cttattacgg
     3241 atattaaaca gaagaataac taaaatcgat atgacaaccg atataactaa atttatcact
     3301 aaacttatca ctttgaaatt tccgatatta acatacgaac aagcaaaata acttaaataa
     3361 gaagcaacaa aagctattac acacaaactt atgagcttcc aaacatattt acctgccgat
     3421 tcaaatttaa atatattttg gaaaacatta tataataacc agggtaaatc aaccaacaat
     3481 actgaaacaa tggttgccgc caaaactcca tataatccga accaattaac gaggatgata
     3541 tttaatacaa tgtttatcat tgctgtaatt aatggtctaa acctgtctgc gtaccatatt
     3601 ccagcagcat ctttgtataa cccaattaac tgatcaattt catataagaa aaaatgaact
     3661 atcataaata taacaaatga aatactcata agattatcac ttcccaccca gataatcatg
     3721 aacggctgaa ataatgtaat aaagcatgca caactaattg ccagaaacca acctataatt
     3781 gttgtaatat cacaaaaatc tatataattt ttttcttgac tttctacaat aagactgtta
     3841 cctatacctg ctatacatga ttcataaatt attgcaaata tactcattat ggcagaaata
     3901 ataaagtaat aattctgata aatcgcaagt actgaaagac ctaagaaaga tgaaataata
     3961 attgaatctg aagatcgtaa tattgtaccg cctaccttgt ttgtaactaa tccttttact
     4021 ttttttataa tatcgtcaat agtttctttc tctaactcac caacaggagt ataatcagga
     4081 tacatctttt gagaaacaac agcacacatt aagttaagaa ctatttgtcc caaaatagaa
     4141 gccaataaat acaaatagta attttttaag aaaatcagaa catatgcctg cattacaaat
     4201 tggaatgtaa aagtaattag catagacacg ttaattacat catttctttg atgtgcatat
     4261 attagactct ttctatatga aaataaccaa tacgacaaaa cagtatttat caaatacatt
     4321 aaatacaaaa ctgtcatatt taaatttcca ggaatcgaac ctttaactaa aaatctcaaa
     4381 aaaggaatgc ataacaatcc taatgtaaac actatccatc caataattga aaagtacttt
     4441 ttatatgcgt tcagtaaagc acaaatctct tttctattgt cttctgctat aggtttatac
     4501 atacttacag ttagtgcagc acctattccc aattccgcta aatttaacat ttgaaatata
     4561 gatgtgaaca ggttgttcaa tccagcatac tccattccca tctgataaat cataagtgta
     4621 cgcataaaaa agggaatgat aatctgatat aacttaaaaa ttgttccagt aattatattc
     4681 cttttagcat tttgtttacg attaattatc atatcatatg gctccaaact ttctattttt
     4741 tattgttacc taatactata taatttctaa aatcttgaaa tgtaataggt ttcttctagc
     4801 tctttgatta attacgctaa ttttcatgtt tggacaacca caaaaaacta tacattttca
     4861 atcaaagcaa cccattgttt tgtaatttct ttcaaattaa atttttgtac attattatat
     4921 gcgttcagtg acattctcat tgctaaatca gtattcttag taaacattat catgctctta
     4981 gctaattggt ctatattctt atcttcaacc aacatcccat taaaattatg atgtaccaaa
     5041 tctctcggac cagttttaca atcataagaa ataactggta atccataaga ctgtgcttct
     5101 aatataacca taggaaatcc ttcataaaca gaactaagca cataacaaga tgcagattta
     5161 taatattcct ccacatggtc tgaatgccct gctatagtta ctctttgtag atttaaatct
     5221 tttatctgct ggcgaagatc tttttctaat tcaccttttc ctacaatgat aagatcccaa
     5281 tcatctactt ttttttctac aattttccaa gcacttatca tgtcagcaaa atttttctct
     5341 tccactaatc gccctactgc aagaaatctc tttttatcat aatgatattc gtactcgata
     5401 ttagatatac acggatttgg catagttata acaaaatctt ctgataaact atattcatca
     5461 atatgttttt gacgatcttc ttctgtgaga accacaagcc tctttgcata tttttttatc
     5521 aattttttag ccttgaatct cttcttttct ttcagcattt ccattgtcca gtaattaaaa
     5581 tgttcccaag aaatcaattt gcactttgtt cctttaattg cccttgtagt atacaaagac
     5641 aacaaagtat caatatcaat tactatatca aattcatttt tgcatatata cttgtgcaat
     5701 ttccaaaccg gatataaata agttctatat aattttccct ccgttttagg atttaacata
     5761 taatcaacct taatcgtctt atctaaccca aaataaggct ctccactatt ccaaaagctt
     5821 aaaatagtga catcataacc taaatcagac aagctattgg caataatact ccctactctt
     5881 tcagttcccc cagaacgaga aattacacca ctaatgaaac aaattttctt caaagtactt
     5941 ttctccttta taagtgtaaa aattttttca gtgcaagtaa cctgttaaat tttttttgtc
     6001 caattgttga ttttaatcta taaacaaatt tttgtttagg tgttttccaa gatgcattat
     6061 attgatgaat gcttattgta ttactagttt tataaatttt tccagtatca taatccttgg
     6121 cagcaaaata atccgtagga tagatatgca ggccatttat tatttgttcg ctgttatcgc
     6181 gaaccaatcc aatagattca agataatctg taatataata cggactcggt ttcaagttca
     6241 atgtaccatc attttgataa aaacttaatg aatcgtaaat atcccttaaa cctttaataa
     6301 cagaatgtcc ttcttctgca ccaaaaccaa taccgccatt aacccagatt tccccttcaa
     6361 atcccatata ggcatgattt tcacgaagta tatctaacga cttaattagc tcaacatctg
     6421 tatctaaata aaatccacca tgattataaa taatatcaaa tcttgcataa tctggaacaa
     6481 aaccccattt acgttcttta tatgcatcgg ccatataacg gttcttattt aaatcataat
     6541 ttgattcatt ccattcaata atctcataat cagggcaata ctttttccag ctttctatgc
     6601 actttctatc ttttttcgga agtggatttc ccccgaacca acagtaatgt atcttcttag
     6661 gaatcataat atctctcctt ttgtattttt ttcactataa taatgctctt tccttttcac
     6721 aacttcaggc atggctaaaa tcatataaat cagtggcccc gttgtttcac ccctaagtgt
     6781 tgtgttatac acaaaaaaga ataccgacat aattgcaata actttaacaa gacttgcatc
     6841 tttatccttc ataaaagatc tttttgcatt gaaaaatgtc tttattataa aaaataggaa
     6901 tatcgaaact cctaatattc caacttctat aaataaccaa atatgatgaa aatactgata
     6961 atgaagatta ccaaacgatt tataaaactc actgacaaaa aaattggtaa actctgcatt
     7021 gccaatgcct actcccatta ggactaatct aaaattatta ttaaatattt catctactac
     7081 tctagagata ccagttattc gatttaaatc accaacacca tcatatccag aatctcttga
     7141 cgcatattct aaaagacctt gaattgtgaa attatcagca tagctttgtc cattatcgtt
     7201 caatgtaata aatattgtta taaaaaagga taatactatc gacacaagta ttacgcatga
     7261 taatatcttg atcactactt taaaatcaac ttttaaagtg aatactattg caacaaatat
     7321 tataaataat tcaggaataa aaactttcaa ttcagctaat gcagacataa aaagtgctgc
     7381 acaaacacat aaaataaaat ggatgaaatt tttttctccc ttgaaatatc cgattgtaac
     7441 aacagtaact gtcacacaca aaattaaatt caatggcata ttacaacctg aataattcca
     7501 aaaaactcca cctatcatat ctcctacaat tgttccagtt ggataggtga ccatgaatct
     7561 ttcaattaaa caaagtggca gtgaaatcca gaataatttt tcaattatag cgaatacttt
     7621 atagtaatct ttaattgtta aatatgttgc acaatttata tatactatga acatacgagt
     7681 gttatttcgt atcccccaga taatcaatcc tatattttct aaattaataa ctgagctaca
     7741 aatagaaact agtaaaaaca aaactatcag catctcattt ttgctagcat atattccttt
     7801 tctgcgtcta aagctaagtg catttaataa cacaaagata ttaattatat ctcccaaata
     7861 taaaattgct cttggggcgt taaaagtata tacaagaact atggagattg caaaaaatgc
     7921 caacgtgaac aatgtccaat ctgctcctga ccatcttttt attgaaatac gcatcgttat
     7981 tcaccttctt tcgctaaaaa ttattctaaa aatacttttg ttaaattaac ataccctatt
     8041 atttttgccg gtacacctgc aagaattgca ccattctcta gacaatcttt gataacaata
     8101 gctcctgccg caacaactac attgttagct atttttacat taccaataat ctttgcaccc
     8161 acgcatacac gtacattatt tccaattaca ggacacttag gatcgattcc atcatttcca
     8221 atacagttat tcccatataa gtaacaattt tttcctatta tggcgttacc attaataaca
     8281 attccttgtg tatggtaaaa tcttacacct tcatcaaata cattttcacc acactctata
     8341 ccacattttc ttcccaaact attcattctt ctgcaagaca caatatacat aatcgcataa
     8401 atgatatttt tctttctctt tgaataataa taagatgtaa ttctcattcg acgagtatac
     8461 cgccaagcgt tgtagtccgg atgtgatttc attattttta gtaattttgt gtgttgtcct
     8521 ttagattcta tatatagatt cttttccata cgaagaaccg cttttaaata ttctttactg
     8581 ctaatttcca ttcatatctc ctattaattc aatttatact taacaatttt attcggattt
     8641 cttgcaacaa gagcatcatt caatgctact gaatctacaa cgatggatat gttatcttca
     8701 tcgttgattc caccggatac aatattactt ataaaaatcg taacattttt tccaaaagaa
     8761 gggatgtccg tgatgtataa tgcccatttt ccgatatttc cagttattta aacctaacga
     8821 atttttacaa tagtacaagt atcacaatca ttaatatata actggaatat aaatctccaa
     8881 aaattcacta tagtacagtt ttttatgatt aaaatctcct atttctaccc attccgctta
     8941 aatatgctaa tgttttaacc ccaataatgg attggtttag taaaaaatag ttaaatattc
     9001 gttgtttaat tggattttgt ttatatgggt atgagtgaat acaggcaatc aactcatcgt
     9061 tcataagtat cttagacaaa taggagttta aatcaaattc tttattaaaa ctagcatatc
     9121 taactgcctt ttgaatacaa gtacgacagc gtcccaacaa aagtctcctt gttttgttta
     9181 caaatatctc attttctaat atatagctta aatcgccttc aattttttta actaattcaa
     9241 tattacaatc gaactcatta tctctaaaat tatgtgtcaa tgaattatta ttattacatc
     9301 tataataata gatacaatct ttaataaaac ttgttatttt tgaatgttgc aaatatgaaa
     9361 tatggaatat gatatcttca gatatataat ccctttctga cggaaacttg atattgtgct
     9421 gttgaattat ctcagatgaa tacagcgcat gccaaactga cattggcaat ataaaatcga
     9481 ctttataact cggctcagtg ccaatcattc caagcaacac tttttcaatt atagcgttat
     9541 ttgtaaatat cgtactatca tatcttgctg gctttgaaat aatattatta tcatcataat
     9601 attcataata tccagcataa catgtgtctg cattactcct ttttatacca tttaacattg
     9661 cttcaatata attagctgca acaaaatcat cagaatcaat aaaagcaaca tattttcctt
     9721 ttacatgttc aagtcctgta tttcttgcca atcccaagcc agcattattt ttatgaataa
     9781 tacgaatcct tgaatccttt aatgcatagt cttcacatat tttattagat gaatccttag
     9841 atccatcatc cacaattata atatctaggt tcttatatgt ttgattacac actgattcca
     9901 aacaacttcc tacatacttt tcaacattat atacaggtat gattacacta attagatcac
     9961 tcgaattcat ccttctatcc tttcactcac gttttctcaa ttagtcaatg acggcatcaa
    10021 cgattttctt aaattctctg aaaaaaatag actctgatct tgatgatgtg aatgacatac
    10081 tatcttcttc tagaaaaatt atttccttta aattatttat taaatcaata atatgatcct
    10141 cgtaagtatg ctttcccttg gaataatgaa aatatacttt tgtattacac aatattgcaa
    10201 ttttatttct taatatgatt atttcactca ttcattactg ccacatatac acaacttata
    10261 accaccccct gcaaagttta gggtaagtta tcagataata tcttcctctt catcatccag
    10321 aatacaaaat ctatttattt atagaaatat cattaatgtt tttatataat gtaccgtgct
    10381 tgtactcttg ctctattaca agcactaaaa cactactatt catttgaatt cttacacgga
    10441 taaaaattta catcgtctat ttcaatattt tttctctcta ttttattcac ttaataagtt
    10501 cattacataa tcgaaacatt ttttattttc cttgtcatca ctccttgctc cctttttgat
    10561 acagatttaa aattctatca aagaaatttc catcgttaaa tttcgttatt gccaaatcct
    10621 tcgaacaacg tgaaaatttt acgtatttat ctttatcatt gatttttagt attgcagatg
    10681 tcaacgctga tacgtcacca ggttgaaaca aatatccatt ttctccatca cgtactgctg
    10741 aagaaatatc tcctacatct gtagcaatta caggcattcc ataagaaatc gcctctagaa
    10801 tagatatagg caatccctcg ttataggacg gtaaaatcat cacatttgat cttctatagc
    10861 actcctcctt tttttctccc acaacccaac ctgtaaattc aaaatacgat tgcaaattat
    10921 attcttctat caattctttg agcgatattt cttcatcacc tgctccagca atctcaaaaa
    10981 taaacttttt ttctggatat tgttctgcaa tgtttttaac tgcttctacc aaatcgtgta
    11041 caccttttcg ctttatcaaa actcccataa acagaaatcg gcacattttt gaattccact
    11101 ttatggtata ttctggaact acaatagaat tttctacaac aacagtttta caatttggct
    11161 caatttcttt aattatcttc ttccattttt ctccaagtac aatcgtggaa tcagactctc
    11221 ttaacagcaa gcgaatctga ttttgttttt ttacagaaac actatcatac cattttttaa
    11281 attccgatcc atgaagatgg atgattacag gaatatttaa taatctaaac cacttattca
    11341 gcacatattt cctactaaaa ctccctctgt acgacatgtg catatgaata atattaggtt
    11401 tactcaataa agagatacct ataattttag caaatgcata cataaaatat aaagatttaa
    11461 atattgcatt tccaccctta taactcggta taaaagtcat ctcgatattc tttttttccc
    11521 aattaaaatt gagcatttgt gtaattatac ttgtaattcc accttttacc gagctgtcat
    11581 taccaatcat aagtactctt tgcataacgc tttactcctt atatttatat tttttctatt
    11641 aatacctcta tcgaattaat aatcgtttcc gtgttagata catacttgtt aaacatccga
    11701 actcttgttt ctttaacggc atcttctaaa ttttctaatt cataacaagg atagataata
    11761 ttcatctcat caaattgtct aattaactgc agttggtgat catcgacatg ctcaccatac
    11821 ttagccaatc gaggaacagc aatgaccctt ttaccttttt ttacggcccc aattatagca
    11881 cctgtacctc catgagtaat caccatttct gctttttgca tagtttcagt aaactcatcc
    11941 ctatttagaa atgctttata ggaataattt agtggtttat aatcactata accactctgt
    12001 gcaaatgtac tttcgattat tactcctttt tcaattaaat catcaatagc ttttaacaat
    12061 ctatcaaatt gaaatttttg tgaacctaat gttataaata tcattttttt ctcctattat
    12121 ttaatatatc ccacccaaat aaatagcatt aggataaaat tgtagcatag tttcccactg
    12181 aacataaaat tgatctgcaa aatgatacat caattttcct gtctgagtag ggctggttac
    12241 ttttgcaaac gattcaatat aaatcagttt ttttcccatc atttttgtta ttaagcagat
    12301 agggatcata gctaaaacac ctgtacaaat cacgagatcc ggtttttcct ttatatatat
    12361 aataattgat ttgaataaat taataagtat agtacacaaa aaaaactttt cttttctatt
    12421 tacttgtctc atgtagtacg ttttttcgcc ttttgactca gctgaatatt ctgtctgttc
    12481 tgttataaca aaactctcat acttctccat cagtggtttt aacatcatta actgttcata
    12541 atgcccccca gaggaagccg caaaacatat ctttttgttc atattaacct ctctatcagt
    12601 cacgctgaaa aatatctcta gtgtaaactt tttctttaat atcatccaag caggtatcat
    12661 atcgattggc aataatagaa tcacacatcg ccttaaactg ctctaaatcg ttgataacct
    12721 tacttccgaa aaaagtctct ccatctttca gtgcaggctc ataaatcacc acagtcgcac
    12781 ctttagcttt tacgcgcttc ataactccct ggattgaact ctgacgaaaa ttatcactgt
    12841 tgctcttcat ggtcagccgg tatataccga ttaaaactgg attttccctg ttgacatcat
    12901 actgattata ttcagcgtag ttgtaatagc ccgccatctg aagaacacga tctgcgatga
    12961 aatccttacg ggtacgatta gattcaacga tagcctcaat caaatttcca ggaacatctg
    13021 catagtttgc caacaactgc ttggtatctt ttggcaagca ataacctcca tatccaaagg
    13081 atggattgtt gtagtgagtt ccaatacgag gatcgagaca aacaccattg attatctgct
    13141 gagtattcag ccccttcatt tcagcataag tgtctaattc attgaaatag ctgactcgca
    13201 gtgctaaata ggtatttgca aacagcttta ctgcttcggc ttcagtgaat cccataaaaa
    13261 gagtatccac gttctcctta attgcacttt tctgcagaag tgttgcaaaa acatgagcag
    13321 catttaacaa gcgctcatca gtcatatcag taccaacaat aatacgagat ggatacagat
    13381 tatcatataa agccttgctc tcgcgcagaa actctggact aaaaataatg ttcttactcc
    13441 ccgttgtctc acggactgat tcagtgtagc caacaggaat tgtagacttg atgaccatga
    13501 tggtatcagg attcacgtcc atcacaacct caataactga ctcaacagca gaagtatcga
    13561 aatgctgagt acgactgtca tagttagtcg gtgtagcaat aacaacgaaa tctgcggatt
    13621 tataagcgct ccttgcatcc aaagtagcag ttaagtcaag ttctttttct gtaagatact
    13681 tttcaatata ttcatcctga attggagact tccgtttatt aatcagttca atcttttcag
    13741 ggataatgtc taccgcggtt acctggtgat gctgcgccaa tagtgtggcg atactcaaac
    13801 caacataacc ggtgccggct actgcgatgt tgtacttttt cttcacaata tctcctcttt
    13861 cctctcttaa gtatggagaa tgttactcct acggctattc atcatgtcaa aataacacag
    13921 cttagaatgt tgataaaata attttccaga atgtgataaa atctctttca tttataaacc
    13981 acacatctaa ttttatctat tctccacaag acatgtagag tattctttaa taacaagata
    14041 cgaccttact aaatacttca aaaaattatc atatttaatt cataaatata aatcttcatt
    14101 ttaaatgtat gcccaaaaat ttatctgtaa tgcgtcatca aaatctagat tccaaatctt
    14161 tatcaatttt tatccactca ttctaccact ttagtaatat tttctattta taacactttc
    14221 gtcctacatt gatccccccc tctgaagtac agtttttact gtcttaaata aaattttaaa
    14281 atctaacccc atactccaat tttttatata ttccgtgtca agttttgcta cttcttcaaa
    14341 atcagtaata tcacttctac cacttacctg ccacattcca gtaattcctg gcttaatagc
    14401 aattcttgca cgatgatgat aatcatattc ttcatactct gatttaaggc atggtcgaaa
    14461 tcctataagg ctcatctttc ctgttagaac gttgaaaaat tgagggaatt catctaaaga
    14521 cgtggatcta ataaattgac cgattcctgt cttttttgtt ccatctggaa gtattttatt
    14581 tccgataatt cttggatcaa actccatctt aaacataaga ttattgctca tcttattttc
    14641 tttcattaat tctacttttc tttcttctgc atccatatac atacttctaa acttatacat
    14701 cttgaacggt ttaccattct gacctatacg ttcttgtgca aagaaaattg ggcctggtga
    14761 attaatataa ataagtggag caataaagat aaaaataatt cccgttgcaa tacatccaac
    14821 aatacctgca agaatatcaa ttgttcgttt tataaatgcc tgttgagttg tcataacatt
    14881 gatacttgtt gtaagtactg tatattctcc tatacgacca atagattgtt ttcccaaagc
    14941 actatctgat acttttgcaa gattcaaatg tacagtaagg cccatttcag agcatcgttc
    15001 aataaattct ttcatacacg aagtactttt attcaaaata ataaagactt catctaccca
    15061 tccctgacat aagtaatccg ctgcattttt ggaatcagaa acaactgtca ctccctcaac
    15121 atcttcacca atcatgcttt tatctatgat aatgacacca tttattttat acattttata
    15181 gttatgctct ttcacattac gaataactac ttcagcaata ctactagatg taactataat
    15241 aagtgatttg ttcccctcat ttttcatttt atgtttcaag taatgtttcc ataagaggcg
    15301 agttatatat gtaattacaa cgtataaacc tcccatcaag aaaagaacga ctctagaata
    15361 atcatttcca ccctgaacac taatgagata caaacttcct aacagcacca acataatcgc
    15421 ttgctttaca gaagaagcaa attctatata gtaccctctt tttaatacac ctctatatgc
    15481 ttcaaacatg aaaataacta aaagatctgc cagttcaatg aagactgcca tgttttgata
    15541 cattggaatc acataaggat tccaatctac ctggcgtaag acatacgata gataaaaagc
    15601 aagctggata cacaataaat ctaaaataat aaaatcaata tgtttaaccc atccccttga
    15661 acttttcttg tacattcctt tttactcccc tctttttaat aaaataaatt gtattacttg
    15721 ttttctctcc taaatatctt tctgaaggat tattgactcg acaaattggc ttgtggatat
    15781 tcttatatat agaacatccg taagccaact taaaactcct taacaaaatt tttttactca
    15841 tattgtagat caaaacaagt atccaagaaa tcatatttta tttctttttt ctgcttactc
    15901 aacaacagct aaaagaaaaa tcatcataaa caaattcaac taagccaacg atccccttgt
    15961 gaaatcaagt tttttcttga tttacgaata aagagctaaa ataaaccaaa cttcttcttt
    16021 tttacctctt catagactgg ataactgatt tcatccccgt tgatcagatc ctttgcgact
    16081 tgtttcatct gtactacttt ttccttacca aagtcctttg caatctgctc atagcactct
    16141 tttaagtaaa aggtgcgctt agagacccca tgggcatcag aagcagccat ctgcacgaga
    16201 ttatgtttga ccatcgtttt ggctgttttc tggatgtctt tgccaaatac cccaacataa
    16261 cttggcgcag tgagttgggc caaacatccc atatctagga acggaagcaa atgatttggg
    16321 tcttcgcgaa aatgcacgtt gcgttctgga tggacgatta ctggtgtctt tcctagttct
    16381 aacaactcaa aaaaaagccg ttctgcatat aacggcactt ccagtgtcgg aaattcgatc
    16441 agaagatatg tatcatctaa atctgtaaat aatatttcat ctcggtggat gtcttcgatg
    16501 agcgttccgg taatacgtac ttcttgtcct tccaataacg tcaaaggcaa ctgtcttttc
    16561 tctaattcgg cttgtaacga agcgactaga gaaatgactt gtgatttttc attgctgtat
    16621 tttccattat tgtggtgtgg tgtgcataaa atatgcgtga tgccttgctg aatcgctttt
    16681 tcagccattg caatacttgc ttctaagttt tctgccccgt catcgattcc tggtaggata
    16741 tggcaatgta gatcgatcat ttctcttgtc tcctctcttc ttattcctta atttccgtag
    16801 taataaccag catctttgct atgttctgct ccgttgtaca caacacctaa gactcgtgct
    16861 tgtaccatct ccagcagttc ttttgcttta gtcaaggatt ctttacgtga catattttca
    16921 cgtacgacca ataaagtgcc atccgttttt aaagacatga tctgtgcatc tgttacggct
    16981 actactggtg gcatatcgaa tatcaccacg tcaaataact gtcttgcttc ggataggatc
    17041 tgatccattc ttggcgagct taacaattct gatgggttgg gtggcttagg tccactaggc
    17101 aaaatcgaca agttctcgac tggcgttcgt tggatcacat ctgctacact tcctgatgaa
    17161 ctaagtgctg tacttaatcc actggcattg ttcagctgga atgtcttata gacgacgggt
    17221 ttacgtaaat ctgcatcgac tagtagaact cgttgacctg atttagcaaa aacgactgca
    17281 atgtttgctg cagtggtgga tttcccttct ccaggacctg atgaagtcac gacaatcgtt
    17341 ttgatttgct gaccagctgc ggaggcaaac tggatattcg tccggatcgt acgatattgt
    17401 tcggcgattg gtgaagatgg gtttgttaat gtgatcaaac tgactgcatg agtttgtgct
    17461 gctttttgtt tctgtgttcg tgccattttt cttcctccta gactcttgag cggcttctgc
    17521 gtgatggggc tgttttatcg tttgtagctt ttggtcgtgc aggagctgtt ccttttttac
    17581 ggataccagc gttcaattct ttcgctgaca tttctggaac tgttcctaag attgggaatc
    17641 ctaatgtatc tgtcacatat ttttcatctt tcactgtccg atcgagtaat tccaataaga
    17701 atgccagacc tacacctacc atcattccta acattaagcc agtcattaaa ttcaatttat
    17761 tatttggcga aacaggtgtc gtattggcaa ctgcacctga aatgatcgag attttatcaa
    17821 cattcatcac atcttttgcg ttctcttgga aaacttgtga agttgtgtta gcgatctgtt
    17881 gagcagccac tgcattcgta cttattgcct gaatcgagaa catttgtgaa ttttctgcct
    17941 gattaactga gatggcatct ttgatttgtt cagcagtcat tttcacattg tcttctgttt
    18001 ctaaacgatc tttgacttca ttgagcacta aatcactgac gatcatatct ttatacgtat
    18061 tgatcatttg caggttcgta ttgacatcat tgacatttgc agtgtctgat tgtggcagtg
    18121 tcacgatcaa ttgtgcttgt gaactgtatt ttggtgtcaa gatgaaaaat gttacgattc
    18181 ctgagatggc caagccagca aacattgaga tgatgattat taccaagtgt ttgcgtaatg
    18241 tttcaaaaat ttctttcaag ctgattgtct cttccatttt ttctccactt tcctatttat
    18301 tctctagatt catttgatct tttaattctt ttttcacacg atcaagttct tgatcactga
    18361 ctttttgata ggagatatta tcttgcatga agccttctcc ttgcagctga tcttgcttaa
    18421 tcgtaccaaa tgcctcccga taatctaaag caattttttt catttcatca aagttcatgt
    18481 ctgttttcat attcgactct agagcattca aaacggattg atagttgctg acaccgttta
    18541 gactcaatac ttttttcgct acgccttcta cgatctttct ttggcgccct tgtctaccgt
    18601 aatcaccatt tgggtcttca taacgcatac gagaataaga taatgcctga tccccgttca
    18661 atgtaatttt accgattgga aagtcatagc catcttgtga gaaagtgagg tcattattga
    18721 cttcgatacc accaactgca tcaacgagtt cctttagtcc tttcatattg atagagacat
    18781 agtgatcgat tgggatgtct acatattttt gtacggtatc catcgccatg gcagctccgc
    18841 cgaatgcgta agcgtggttg atcttgtctt ttgtgtcacg acctacgatg tcgacatacg
    18901 tatctcgagg aatgctgacg atcgttgtct gtttgtcttc aggactgact gtggcaacca
    18961 tcatcgtatc tgatcttcct tgatcgaccc gacctaaatc tccagtatca atccccatca
    19021 atagaacaga gaaagattct tgtttgtcca gatcaactgg tttcgttcgt tctccttggg
    19081 ttcgttccac tgattcgtaa gttttctcaa tagaatgtga gagatccaga tataattttg
    19141 ctcctactcc tacaattgct aatactaata cgataagaac tgctaaaata atcaaaaata
    19201 tttttttccc tcttgatttt cttcttcgtc ttgccatagt ttcctccgtt tgctaacctt
    19261 gcttgaatca acgttttaac tgtcaatagc ttatattgct atagcccatt tgctgagcaa
    19321 cagtttgtgc ttttgttgcg tatgcggctc ttattttttc attccggatc tgctctattt
    19381 gactgagcgc cgtcaaatat tgatcgtaag tagcggtagc tgttacttga ccgtctttca
    19441 gcatcgtatc gaatagctga tctagttgcg tgacttgtgc aatttgggta tcagcgaatc
    19501 ctagatattg cataacgact gttttccaag cactatcctt aaaaaagctc atattttcac
    19561 gtacatgggc gacatctgca cttgccaact tttctttgat gatgacatca tccacagctt
    19621 gctgccagtt tgatacgttt ttagtaaata atttattgac agccgtctgg atcttcagtt
    19681 ttgattctgc atcctctaac tgcgttaaga ctgctttctt ttcttcttgg atcgcagcag
    19741 cttctttagg taattctgat tccttgatgt cataatcatc tgccgaaagt ttgatagaag
    19801 atagtttatg tgacaaattt gaaactgttt cttctgttat ttcaggattt aggaaaattt
    19861 tttcttttgt actgtacagc ttatctactt cttttgataa agaggtcacg gtgtcttttt
    19921 gattggtgat ggcagattct gcgtaattga tccgttgatg atattgccgc tgattattga
    19981 gatggtatcc aaacaagcaa atcacaatag cgcctgtgcc taccgccaca gtagccaata
    20041 acttgacgaa cggcttttcc atagattcac tcctaaatat tgatgataga taagtaaaga
    20101 tgtgattcat tcacatcaga atgagcgctt tttattttaa aagcataatt aaactaatat
    20161 attcatagaa atatttataa acaggttttc cctatataat cataccacat acagtttttt
    20221 tgactaggga aattcctaat ttaaaagtaa ataaaacagt aacaagatta taattaatcg
    20281 ttttcatttt tatttcctca cttttcatga gcgttttgaa aattcgaaca aagaataaaa
    20341 taacaaaaat tattaaaata gaattaataa cagctattta taacaaaaaa aacagagaca
    20401 cagcacgttt cgtgcatgct ctctgttttt caaacaaaag actattttgt gatgatttga
    20461 tccaagcttg ctaatgacaa agccatggca gctatttgac ttagttgtgt tagtcggttc
    20521 ttacgtactg ctggttcatc agccatgacc atcgtttgat caaaataatt ttcgatcagt
    20581 ggacgaagag cgacaagctg gtcgtagttt tcgtggatcg tttggtcagc aaaacttgct
    20641 ttcattgctt ctactgcttt gtgtaattct ttctctgcgt cgttttcgaa cagctcaggg
    20701 tctacttctg attgtttttc aagctgcgct tttttcgcta agttcacgac acgtgtcaaa
    20761 gcttccattg aaggtttgaa gtcttcatct gcaagatgtt gtttgaacaa ttgtgctgct
    20821 gcaaatactt ttgtcagatc tttttgttcc gcattcaaaa cagcatcgat gacatcatga
    20881 cgaatattct tcgttgtcaa taattggcgc agacggcctt tgataaagtc gatcacttgt
    20941 tgttgccctt ctgataaacc aatgccgaaa cgatccgtat ctttactgat aacatcgcta
    21001 atcgtttctt gcagaacaga caaaggaaag gcccattttt tcgattcgat gatccgaacg
    21061 acaccgtatg cttggcgacg taaggcataa ggatcgtttg acccgcttgg aatcatccca
    21121 actgcaaaga aagaaaagat gctatctaat ttatctgcaa tcgctaaaac agaacctact
    21181 gtagactgag gtagctctcc ttcactggat ataggcatgt aatgctcacg aatcgctgtc
    21241 gctacagcag gtttctcgcc tttcagaaga gcatattttt cgcccatgat cccttgtagt
    21301 tcaggaaatt cccctaccat gttggttact agatcaaatt tatagatttc agcagctcgt
    21361 tgcaaatcca tcaattcttc ctcagatagt cctacttgtt cgccaatgat acgggcaatg
    21421 acttgtaccc gttgcatttt ctcatagata ctaccgattt tttcatggaa agtaacagtt
    21481 tttagttttt caacgtattg gtcgattgaa gctttcttgt cttcattata aaagaattcc
    21541 gcatcttcta aacgagccgt caatactttt tgattgcctc gaaccacatc ttccaatttt
    21601 acccgatcac cattacgagc agaaataaaa tgaggaagca gcataccttg atcattccgt
    21661 acctcgaaat aacgttgatg ttctttcata gaagtgacta gtacttcttc aggaacagaa
    21721 agatattttt cttcgaagtc gccagaaaag acagttggat attcgactaa attgttgact
    21781 tcttctagta aatcttcgtc tagatcaata acccagtgat ttttttctgc taattcgttt
    21841 gcttgtgtaa cgatcatttc ttgtcttttt tgcggagacg caatgacgaa ttgttcattc
    21901 aatttttctt catattcttt tgcatgcgca aatgtcacat cttcgcctaa gaaacgatga
    21961 cctcgagagg ctctgcctgt tttgacatcc aagatttcga atgggattac ttcgtcatct
    22021 aacaatgcca cgatccagtg aatagggcgg atatattcaa aatcgtattc tgcccaatgc
    22081 atcgttacag ggaaagtcaa acttttgatc acccctgcta agcctgtcaa tacttctttt
    22141 gcttccttac catgagtgta tttcgttaca tagacatatt ccacaccttt taattctcta
    22201 aaagtgatag cgtctgtcgt cagaccttgt ccgcggacaa atccttccgc tgctttcgtc
    22261 cagtttccat ctgcgtccaa agcaattttt ttggctggtc ctttgacttc ttcttcactg
    22321 tccgcttgtc gttctggaat atttgttact ttgattgcta aacggcgcgg tgtggaaaat
    22381 gtttcgatcg tatcataagt caattggttt tcatctaaaa attttgctgt tttttcttct
    22441 aattgtttca tgcttggtgt gacaacatgg gcaggcattt cttccagccc gatttcaagt
    22501 aataagtctt ttgccatctt attcagcctc cttcacggtt tctggatttg ctgtgttttt
    22561 attcaacaaa gggaatccta atttttcgcg ttctgccaca aagattttgg ctacagcacg
    22621 agccatgttg cggatgcgag aaagataacc ggcacgctct gttacggata ctgcaccgcg
    22681 ggcgtctaat aagttgaatg tatggctaca tttcaagata taatcatacg ccggatgaac
    22741 aagattttca tcgatacagc gtttagcttc tttttcaaaa cgatcaaagt tgcttagtag
    22801 caagtcttgg tcgcttactt caaaagaata tttggaatgt tcatattctg gttgtttgaa
    22861 gatctcgcca tatttcacgc catcagccca ttctaaatca tagacacttt caacttcttg
    22921 gatataagaa gcaagacgtt ctaatccata agtgatctca gaagttactg gtttacaagg
    22981 cagtccacca acttgctgga agtacgtgaa ttgtgtgatt tccattccgt caagccatac
    23041 ttcccaaccc acaccggcac agcccatgga tgggttctcc cagttgtctt caacaaaacg
    23101 gatgtcgtgt tctaatggat cgattcctaa aaggcgcaag ctttctaaat aaagctcttg
    23161 aatattctca ggtgaaggtt tcatcaccac ttggaattga tgatgctgat acaaacggtt
    23221 tgggttttca ccataacgtc catcagctgg tcggcgagat ggctctacgt aagcagcatt
    23281 ccatggttct ggtccgattg cccgtaaaaa ggtatatggg ctcatcgtac ctgctccttt
    23341 ttctgtgtca taagcttgca tcagcatgca gccgtttgat gaccaaaatt tttgtaatgt
    23401 taaaatcata tcttggacgg tcaatttttt actcatctgt tttgcccctt tcaattttaa
    23461 agcgttgctg tcaaataaga atacaggaaa gaaaacgaca aaaaatccct atgtctgatt
    23521 acagacatag ggacgaataa ttcgcggttc caccctactt ccgaataaag attcggcagc
    23581 ttcatttatc gtactcgcaa gtgcctcttc aaaaatcgtt tgggtccggc tcccactctc
    23641 ccgaactcgc taaaccacgt gttttttact gatcttggtc aatgtacgta ttcattttgc
    23701 agttttcttg tttctataat agatcctatc actgaacgat ctatttaatc tatggttaat
    23761 aatagacgga actccctgtg tttgtcaaat ggttttgatt ttttgttgta tgttttattt
    23821 actattcttc tttttcaggt gtctcttttt ctttttcaga catgtctttc tctgacgtct
    23881 ttaatacgtg tgcccaagac ttcatttgat tgatgaactt tttgcttttc aaatggatac
    23941 cgacatattc atcatacagc tggtcgatga gttcacggat tgcttgtttg gtttcttctt
    24001 tcagatggat tccactaatt ttatcataag aaatagctga aaacatccga acgaaataaa
    24061 cagctcgagg atctgcatga taacggtgat cgtccatcga ccaatgtctt tcgcatatca
    24121 caccaccata tttggaagaa tagtcaaatt ttccttttgt ttccccgcat acgacgcagt
    24181 gtgtccagat tggtgcaatt ccgaagcgtt gcagtatttg gacttcaaaa atattcgtga
    24241 tgatttctgc atcgtctcct tggtccatcc gttgcagtgc ttgttgcgta aactggaaca
    24301 aatgcgggtc gtactgatga tcttcgattg ccgcatctac tagattcaat aaatatgtgc
    24361 catagccgct caaaaagatg tcttcttgga tgtgtcgaaa cgcatggaca tctttgacac
    24421 tgcttaaaaa tgacaaacct tcacttcgaa aattcccgat ataaactgct tcagtaaaag
    24481 ggagcaaagc cggagctaag ggctgatttt gccgatggat ctctttgata taaaacatca
    24541 ttttgccaga agattctgta aagattttga ctaatttatc tttttctttg taattacggc
    24601 tagataaaat caagcctttt gattctgcaa gctggctcat attctccctc ctaaaaaata
    24661 aatctggaaa agcagattct gccgatccag atcatttatg ttgcgatctt ccgcagcttt
    24721 tctcaacacg cggattttta ttcagtttat ttctatacct aatttacaag aagctcagaa
    24781 tatcagtatt cgtcttttcg gtagccgaag tcttgcagat agactttttt gtctcgccag
    24841 tctttttgaa ctttgaccca aagttctaga aagactttgt ctcctaaaag attttcgata
    24901 tcttttcttg ctttcgttcc tacttgcttc agcatttttc cgcctttgcc gataatgatc
    24961 cctttttggc tatctctttc gacaataatc gtagcctgga tatggatttt atcattctcg
    25021 tttcgcttca tcgaatcaac aacgactgcg acagaatgag gcacttcgtc tcgtgttaaa
    25081 aacaagactt tctcacggat caattcagaa acgatgaagt attcaggatg atcagtcact
    25141 tgatcatctg gaaaatattg tgggccctct ggcatttcac tgactaatac atccatcaaa
    25201 cgttcgacgt tgtttccttc tgtagcagag attggaatca cttgtgcaaa aggcatctgc
    25261 gttgaataat cttcgatgat tcccaacaac tcgtctggat ggattttgtc gattttattg
    25321 ataatcagat aaacaggaga atgcacgttt tttagacgtt caataataaa atcgtctccc
    25381 cgaccgcgtt tttgatcagc actaatcata aataagaccg cgtccacttc tttcaacgca
    25441 ctgtaagctg cttctaccat aaaatctcct aaacgatgct ttggtttatg gattcctggt
    25501 gtatcaataa aaatcaactg agcttctggt gtagtgtata ctccttggat tttatttcta
    25561 gttgtctgtg ccttgtcact catgatggca attttttgac ccacgattcg attcaataat
    25621 gtggatttcc ctacatttgg gcggccgacg attgcgacaa accctgattt atgttctggc
    25681 atatatgttc tcctttttaa ttaaacaaca aatgatatat tttagggaca aataaaatca
    25741 acccaacgat gacggtaaaa caagcggtca ctaaaactgc tccagctgcc atatctttga
    25801 tttttttccc aatcggatga aaatgaaaat ccgtaaacat gtccacgaca ttttcaaaaa
    25861 cagtattgat gatctctact atccatacga gaaaaacagc taaaagcagc cataaccatt
    25921 ctatccgatc aagttggaaa atcacgccca taaacaaagc gaggattccg aacgcaacat
    25981 gtttcctcat gttgcgttct tcctcaaaaa cagttttgac tccttggacg gcaaattcca
    26041 cggacatcag gaaatgtttg tttttctcta cgtttttatc ttttaagtcc ataggcatct
    26101 aagatctctt tctgtaaacc aaacatttct ttctcatcct ctggtgtcat atggtcatac
    26161 ccgttgatat gcaagaatcc atgaaccgct aaaaagccaa gttcccgatc atacgaatgg
    26221 ccgtaagctt ctgcttgctc tgcagcacgt tcagtagaga tcatgatgtc tcccagtaca
    26281 cgaggcaatg gattctctcc atcttcaaaa atcactggga tttcgtcttc gccctcttct
    26341 tccaaggcaa aactgatgac gtctgtcggt gcatcttttc cacggtaatc acgattgatt
    26401 acttggattg ctgcattgtc catgaaagta acagacattt ctgtttcttc tggcaactct
    26461 aaatatgatg ctgcaaattg cagcaagtcg tcgatttctt tcattttctc ttctgagact
    26521 tggtttgttt catccatgaa ggtaatatcc attatacatc gcttctttct attcttcttg
    26581 ctattcttct tgttcttctt ttatcttctc aacttccgat ttcattttag gatatttgat
    26641 ccgagaatgg aaggtactgc ttaaaccgct gatcaatgat ttttctactt tacggatttc
    26701 ttttaacgtc aatccactgt cgtccaattg tccatcggaa atacgctctt caataagatt
    26761 atggacaaaa ctttcgattt tttcgctcgt tggatggtcc attgcccgta ctgccgcttc
    26821 acaactgtca gctatattga cgattcctgc ttcacggctt tgaggttttg gtccaggata
    26881 acggaattgc tcttccgtaa cttccgggtt gcgttccttt gccttaacgt agaaataacg
    26941 catcagcgtg gttccatgat gctgtctaca aatatcgatc accatttgcg gcatattata
    27001 atcttccaat attttggcac catctgtcac atgtccaaag ataatatgtt tactgtcttc
    27061 tggcaataag aaattgtgcg gattttccgc tcctgaaggc aagttctcaa cgaaaaagct
    27121 ggcgtgtttg atcttgccga tatcatgata atagcaagct actcgagtta gtaaagaacg
    27181 tccaccgatt tctgctacag cattagcact tagattggct accatcatgc tatggtgata
    27241 cgtacctggt gcttcttcca gcaactgttt caataaagga tgattgggat tgctgagttc
    27301 atttaatacg atcacactat catcattgac catcaattca atataggggt gcagacctat
    27361 cgtcagcaag aatgaaaaca gtgcacctgt gaatccgcag actagcgtta accaagtcgt
    27421 tgcatcccca aagctcattc cttggtagat aatcaaaatg acatccatca tcacaggaaa
    27481 aataatgacc cacatagcag cggataaccc ttgttctgag atccgtttgc gtctgacgat
    27541 cgttgccatg agaccagaaa atacatagga catcaaaata actgtcagaa agttcgtgcc
    27601 aatcgaatca taaaaaatga aaagtgcgga tactgcttga aaaataccag ccatgatccc
    27661 tgctcttcga ttcaagaaaa agcttaaaac aagtggagca aaagccgcgg ggtaaaacag
    27721 cggaatataa gcagcttgtt ctgtttggaa gagttggaaa aatttcatta gtacgacgct
    27781 tactgacatt gccgtcacgt agaaaagtac gtattttgtt ctttctcctg cattttggaa
    27841 ttgaagtgaa ttataaatca gtacaccgat ttgaagcaaa acagctaaaa ccatggcaat
    27901 gagtgggaaa atagaagtcg tttgattcgt caatcctagt aattccaatt ttttcatcgc
    27961 tgaagcatca atttgatttc cttcgcgaac aataatttct ccttggaaga tcatcactgg
    28021 ttgtacagct tctcgtgcag attgttttaa ttcttctgtc tttttctcat tcaatacatc
    28081 attaactacg atgccttgat ccactaagta gcgaatcatt tgttgctgct ctgttgtgac
    28141 attcaaatat tgaatctgat cttctgcttc ttgtttaaaa gcttccaatt cgctttcacg
    28201 aacttgttta ctcatctgtt cgtcaatgag cttcaaactt tcatccctaa ctgtgtgaat
    28261 ttccgtttca gtcatttcaa atatcgtcga ataaaaattg ttcggcagtt tttggtaaaa
    28321 cgcaacgttt tctgcattca cgttttcaaa atttttcttc agtgtagcaa tccgctcttc
    28381 tgtagtaggt ttcgggatat tttcatcttc cttcgcctta tctaccttgc cattataaga
    28441 tttgttgatg gtatcattcg tcttatcgat caattcgaat aattgcttga tccgattgtt
    28501 ctggtcatct gccagatctt tttgataagt atattctgga gtcactgctt ctgctgccag
    28561 ttttcttctt tgttcggttt cttccgtgtt ctcaatcgtt ttattcgcac ggatactctc
    28621 ttctgctact tggccttctc tgaaatcaat ggccttttgt ttgacactac taaatgtaac
    28681 gccgcagaca atgataaaaa acaagaacag aaggaaaggc aatgctgcct ttccgagttt
    28741 ttcttgtagt ttctgcagtg ttttattcaa catgtgctta tcctcctaag caaagcttat
    28801 tcttgatgaa gatccgcctt ttcataagct ttgatgattt cggcaactac agggtgccga
    28861 acaacgtcac ttgcttcaaa attaacaaaa tcgatttttt tgatttgctt taatgttcgt
    28921 tctgcatgaa caagaccact tgtcgtccca cgaggcaagt ctatctgact tgtatcccca
    28981 ttgacaatca tttttgattg ataaccgaga cgggtcaaga acattttcat ctgagcgact
    29041 gtcgtgtttt gtgcttcgtc caagataacg aatgcatcat ccaatgtccg cccccgcatg
    29101 taagctaaag gtgcgatttc gatcacgcct cgttccatca atcggttcgt atgatccaat
    29161 ccaaaaattt ggtataaagc atcataaaca ggccgcagat aaggatccac tttttctttc
    29221 agatccccgg gtagaaagcc taggttttcc cctgcctcca cagcgggccg cgtcaaaatg
    29281 attttttgca cttcgccttt ttttagggca gcaattgcta atactacggc aagaaacgtt
    29341 ttccctgttc cggctggccc tacaccaaat acgatgtcat gtttggctac cgattcaacg
    29401 tatttctttt gaccgctgtt ttttacccgt ataggttttc cattacggtc tttgacgatc
    29461 tcttcctcat acatctcaac aaaataatct aatgtacctt tttgccccat tttcaatgcc
    29521 gtcacaacat ctggcgtact tacgtggatt cctcgtttga tcaattcctg taatgtccga
    29581 atgactgagg cagccaagga aatctgggat tgctcaccaa tgatctgaat gatttctcca
    29641 cgtgtatgga tggttgtttc cgttgtttcc tcgatcagtt ttatatgctt gtcgtgtgca
    29701 cctaaaagca tgctgacatc atcagcagct gtcaatctaa tctctaaaga actagactct
    29761 tccgtcaaaa atagacatcc cctttttttc gattcattct ttcctatttt accatactca
    29821 gaagaaacta tatagcaaat aagaaaaggt tgtaaaagag tcgcttaacc atacgtacca
    29881 agaaacgtta aaaaatagcc gtttccattc gctaaaggtc tcgttttgat aaaaagaagc
    29941 tgagaatgta agttatatga aagtggagaa caaaatattt cgtttcattc aacaagtatt
    30001 ctctaggtat ttttcctgtt tcaaaaaaag aaaacaaaat agttaaaata ttcttgcatt
    30061 tcaaaaaaaa caggtgtatt attacgtcaa caaataaaca tacagcaaag cgtcgaggaa
    30121 aacagtaggc tattcaacga tttattccca gtgagtcatg taagtgagaa atgacaataa
    30181 atcgaataga cgaacatcat ttccgagtca atgtcgaaaa cgtagatcat tgtggctgcc
    30241 tccattatca ggctagcgga taactttatc aaaaagcgtc cgtgaatgaa gaagcttttt
    30301 taagcgaaaa aaggtggtac cgttctagaa tctagagccc ttttcccaag tggaaaaggg
    30361 ctttttttta ttttaaggtg gtggttttca tggaatctga tattagtggc attactaatt
    30421 gctgcaacgt ttatatcgtc aatcaaaatg ataattattg gaggaaaaat ttatggaaga
    30481 aacaaaaact tattcatgga aacaactatt gatggtcagt tcacttatct tcgggatgtt
    30541 cttcgggtca ggtaatctaa tctttcctgt ccacttaggt caaatggctg gcgcaaactg
    30601 ggtcagtgct ggcgtaggat ttgctatctc aggtgctctt ttcccattac tagctatctt
    30661 agctgtagta gtgacaaaaa gtgatggtct gtatgactta gctaaacctg tcggaaaatg
    30721 gtatgctgcc ttgttcttaa tccttgttca tttgacgatc ggaccatttt tcggtacacc
    30781 acggacagct gctactgctt ttgaaatggc tgctaaacca tttttgcctg aaaaatataa
    30841 ttcattaggt atgtttttat tctctgctct tttcttttta cttgcttatt tccttactgt
    30901 ccatccaaca cggttggtca aatacgtcgg aaagtatcta aactccttgt ttcttgcatt
    30961 attagcaatc atttttgtcg tttctttatt ccatccaatg ggaaatctaa atcaatctgc
    31021 tgcagatact tatcaaacaa acgctttaac aaatggtgtc ctagaaggtt ataacacggt
    31081 tgatgcagta gctttgcttg ccttaagtgt cacttttgtc catgcagtcc gtgaccttgg
    31141 tttcaaagat aaaaaagtca cagaattaac ggcaaaagct ggtagcctga gcgttgcgct
    31201 agaagtcatg atctattttg gacttgttct tctcggtgcg atgagtttga ataagatgtc
    31261 tttatcagaa aatggcggaa ctgcactttc tcagatcact aatttttatt taggtaactt
    31321 cggcgctagc ttcttaggag tgatggtcac attaggtgtc ttcactaccg ctatgggact
    31381 cgttgtttct tttgcgcaag atttccataa actgttccct aaagtcagtt atatgacatg
    31441 gctgcggtta acgactttcg tctcttttgt cgttgcaaat gctggtttag acaatatcat
    31501 ccaatggtct ttaccagtat tgatgctttt gtatccattg tcgcttgcgt tgatcttgct
    31561 ttccttaaca gcaaaatttt tccaaaagac accatttgtc tatcaagtaa cgatgttgtt
    31621 tgctgctgtt cctgccgtat tggatatgct ggcaaattcg cctgctttag tgagtcaaca
    31681 aagagttgta gcaagcatgc tcgaatttta tcatcatcac gttccttttg ctgcattagg
    31741 acttggctgg atggtcccaa cgttacttgg ttacgctggc agcctactgt tttattacgc
    31801 ctatcgccta tcagggtata aacaagaagc aaatgaacta ccagaagaat aaccaaatca
    31861 aacacaattt aacataagaa atattaaaca ggaaaaaacc agctagatga aagaatccta
    31921 ttctttcgtc tagctatttt ttagttctct tttttacgcg cacaaaaaaa ctgcggacaa
    31981 tgattgtccg cagtttaaat gggctctata atatctagtg ttggtgacgc cttctgttgc
    32041 caatactttt tcttttttct aataaacgca aacagacacc ttgaaatcct ttagaataaa
    32101 gtcgacgaaa accattcaaa ggagtgtttc aagatgtcct attcagagtt taccaaagag
    32161 ttactagata ttctagacct aaatcttatc tttcatgaag atgcctttcg caaagagcga
    32221 atcaatgacg aaacatgttt tgtttttgat ggcacattga cctatcaacc agaggaatgc
    32281 ttccattgtc actatcaaaa taagcaaacg atcattaaat ggggctggaa aaaagtatcc
    32341 atcttattga atgatgtcag taactacaag accattctgc ggattaacaa acagcgcttc
    32401 aaatgtaaac actgtggaaa gactttttta gcagaagatt cagttagtga tcgtcgctgc
    32461 tccattgctc gcagagtcaa acaagccatt cttgagctat taagcgaacc tctttctatg
    32521 tctttgatcg cacgtatgaa gcacatttct ccgaccactg tcattcgaat a
//
LOCUS       DACGLE010000025        32298 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0534
            SAMEA3304449-rid9560453.denovo.026, whole genome shotgun sequence.
ACCESSION   DACGLE010000025 DACGLE010000000
VERSION     DACGLE010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304449
            Sequence Read Archive: ERR985064
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 32298)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32298)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702225.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702225.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 20:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,045
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 27 of 67
            Pseudo Genes (incomplete)         :: 25 of 67
            Pseudo Genes (internal stop)      :: 19 of 67
            Pseudo Genes (multiple problems)  :: 4 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32298
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304449-rid9560453.denovo.026"
                     /strain="VREN0534"
                     /db_xref="taxon:1352"
     gene            complement(124..1227)
                     /locus_tag="IXT20_001288"
     CDS             complement(124..1227)
                     /locus_tag="IXT20_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate oxidase"
                     /protein_id="HAQ4859744.1"
                     /translation="MEKTYQAGTNEGIVDFINMEDLEIAASQVIPAGGYGYISSGAGD
                     LFTYQENERAFNHQLIIPHVLRDVELPDTTTHFDKETLTAPIIMAPVAAHGLAHVKAE
                     KASAKGVADFGTIYTASSYASCTLEEIREAGGEKAPQWFQFYMSKDDGINLDILEVAK
                     RNGAKAIVLTADATVGGNRETDRRNGFTFPLPMPIVQAYQSGVGQTMDAVYKSSKQKL
                     SPKDVEFIAAHSDLPVYVKGVQSEEDVYRSLESGAGGIWVSNHGGRQLDGGPAAFDSL
                     QYVAEAVDKRVPIVFDSGVRRGQHVFKAIASGADLVAIGRPVIYGLSLGGSTGVHQVF
                     DFFKTELEMVMQLAGTQTVEDIKKIKLRENRFI"
     gene            complement(1449..1919)
                     /locus_tag="IXT20_001289"
     CDS             complement(1449..1919)
                     /locus_tag="IXT20_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4809 family protein"
                     /protein_id="HAQ4859745.1"
                     /translation="MKEVQLIRKSELSEGGCNACGVVEATSYTLKLGANKAIISELTV
                     GGLVDSLALAEGFIGEDIYEMFSEIRQLKKGENCIEVHHESPNVRFKRGDNEMIFNNH
                     VSDHTELYGIVNQILTELFGLGPYAFKEENGNPKLNEEWQETIEIQRNNPHLFQ"
     gene            2176..3495
                     /locus_tag="IXT20_001290"
     CDS             2176..3495
                     /locus_tag="IXT20_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1380-like element IS1678 family transposase"
                     /protein_id="HAQ4859746.1"
                     /translation="MTSLHKNQVKFNSNITISHTGGQLSSDSGLVLVKELMNIFGFSE
                     LAKQHIHIEDERAYFTHDNLSILEQFIMQLIAGYSADSAANLLRQDPVFKAVLDRKEL
                     ASQSSLSRFLDRLSEENIHELQALNQELIDKARLIRNDTELIIDLDSTHSDTFGHQEQ
                     TNYNTHYQTYGYHPLVAFDGLTGDFLKAELRSGNQYTSKGVKEFLTPLLEHYNHSLPN
                     TDILVRGDSGFATPGVYDSCESKKSHYVIRLKNNRRLGQIAEKSVLYGDNQKWEEREV
                     QYFSTTYQAQSWSQSRRVCIRSTREAGELLFRHEFIVTNLSENVSPDTIFSIYAKRGT
                     MENFIKEAKAGFYFDKTDSPRFLENHVRMMLSLIAYNLVNFLRTIGFEEVQKGMTIHS
                     IRLKFLKVAGKLVQTGRRVYLKLSSYHVYQNEFYRVFARLRRASQWI"
     gene            3936..5576
                     /locus_tag="IXT20_001291"
     CDS             3936..5576
                     /locus_tag="IXT20_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent malic enzyme"
                     /protein_id="HAQ4859747.1"
                     /translation="MLTGLNLLNNPFLNKGTAFTKEERAKYGITGMLPSTVQTLEQQS
                     VQAYGQYLSKQTDLEKRIFLMNLFNTNRTLFYKLMGEHLVEFMPVVYDPVVADSIEQY
                     NEIFLKPQDAVFLSIDEPENIKASLKNAADGRDIRLIVVTDAEGILGMGDWGVNGVDI
                     AIGKLMVYTAAAGINPAQVLPVSIDAGTNNEELLNNPLYLGNRHARVEGETYYEFIDQ
                     FVQSATELFPELLLHWEDFGRGNAATILEKYEDKITTFNDDIQGTGIVVLAGVLGGLN
                     ISGEQLKDQTILTFGAGTAGVGIANILLDEMIRQGVPEEEARQHFYQVDKQGLLFEDT
                     KGLTPGQIPFARKRSEFTNADELTNLEAVVKAIHPTIMIGTSTQPGAFTEEIVKEMAA
                     HTPRPIIMPLSNPTKLAEAKAKDLIEWTDGKALVGTGIPAADVEYNGVTYQIGQANNA
                     LMYPGLGLGLIASTATRVNAEIISQASRALGGIVDVTKPGAAILPPVAKITEFSQTIA
                     ETVAKSVVAQKLNREEITDIKEAVESAKWVPEYKSLED"
     gene            5730..7052
                     /locus_tag="IXT20_001292"
     CDS             5730..7052
                     /locus_tag="IXT20_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxycarboxylate transporter family protein"
                     /protein_id="HAQ4859748.1"
                     /translation="MAQKTTTATAVEEKKKEGFWSTKITGVSLPLYLIMVLVLIIAMA
                     LGKLPTNMIGPIAVLVIFGNLFHFIGTKIPIVKSYLGGGSVFAIFASAAIATFGILPE
                     YVVKSTENFVSNMGFLDFYIAALITGSILGMNRNLLMKASVRFIPVALISMVVSFFAV
                     GLVGMLIGNGFANSVLYISLPAMAGGVGAGAVPLSTIYANVLGTDASSIISRLIPATA
                     MTNVLAIIGAALVARAGQSMPKFNGNGKLMEKGDLGDGKPRNVKPSIPQLGVGLMVSL
                     TFFILGQICNYFVPKVHAYAFMIIIVVICKIANLLPEYYEDAAIMFNNLIVKNLTAAV
                     LAGIGIALLNLNVLASALTWQFVVLCLTSVIAISVVSGFVGRLFGLYPIESTITAGLC
                     NNSMGGTGNVAVLSAANRMELIAFAQMGNRLGGAIVLIISGFLMQLLS"
     gene            complement(7114..7545)
                     /locus_tag="IXT20_001293"
     CDS             complement(7114..7545)
                     /locus_tag="IXT20_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ4859749.1"
                     /translation="MLREMRLGDAEALREINAEQLGYDVPLTVTVRQMKKLLKEPEKN
                     FFLVYEDPTSKKAAGYVQAESYESIFSDPMFNIMALAVSKHAEKRGIGKALMTGVEEA
                     KRRGISAIRLNSAEYRVEAHRFYERIGYHSDKMQKRFLKIL"
     gene            complement(7609..8445)
                     /locus_tag="IXT20_001294"
     CDS             complement(7609..8445)
                     /locus_tag="IXT20_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HAQ4859750.1"
                     /translation="MIGLIYFVVIVLANTVGAISGMGGGVLIKPILDLIGAHSVAGIS
                     FYSTVAVFTMSIVSTVRQVSSGKSLNWQIVGWVSSGAVVGGVVGNIVFEVFLQLFENE
                     KHVQLIQIFLTVLTLVFAFFYTKHHQPKFHLTSWTWYLICGGVLGFLASFLGIGGGPV
                     NVSLLMLMFALPIKEATLYSLSTIFFSQLAKLVTIALTSSFMRFDLSMLFYVIPAAVI
                     GGLWGARFSRVLSPKKVTFIFQAIVIVVLLINLYNAFVILQTLEGSGFKQVKITEKSF
                     FK"
     gene            complement(8635..8937)
                     /locus_tag="IXT20_001295"
     CDS             complement(8635..8937)
                     /locus_tag="IXT20_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HAQ4859751.1"
                     /translation="MTEHNHSHEHEGHEHITLVDDQGNETLYEILLTVDGQEEFGRNY
                     VLLYPAGVSEDEDVELQAYAYIENEDGTEGELEQIETDAEWDMIEEVFNTFMAEEE"
     gene            complement(8963..9394)
                     /gene="ruvX"
                     /locus_tag="IXT20_001296"
     CDS             complement(8963..9394)
                     /gene="ruvX"
                     /locus_tag="IXT20_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HAQ4859752.1"
                     /translation="MRVMGLDVGSKTVGVAVSDPFGWTAQGVEIIRINEAEEEFGFDR
                     LKELVAQYEVDRFVVGLPKNMNNSIGPRAEASMSYGEKIKELFSLPVDYQDERLTTVQ
                     AERMLVEQANTSRAKRKKVIDKLAAVMILQNYLDGSGKQLF"
     gene            complement(9391..9657)
                     /locus_tag="IXT20_001297"
     CDS             complement(9391..9657)
                     /locus_tag="IXT20_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HAQ4859753.1"
                     /translation="MGFTDETVRFGFDDSRKKEVSETLAIVYRALEEKGYNPINQIVG
                     YLLSGDPAYIPRYRDARNLIRRHERDEIMEVIVKDYLSNHGVNL"
     gene            complement(9911..10636)
                     /locus_tag="IXT20_001298"
     CDS             complement(9911..10636)
                     /locus_tag="IXT20_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HAQ4859754.1"
                     /translation="MSKKTTKAIVGIVAAVIAGALGISTTQKDSDIFQQITTIFDSTP
                     QASQVADVPEYDGKHQEIEINGNQPEFTKEDLSLSKGTWESYSNIDKLNRVGQANAML
                     GKDLFPTEKREPLYIDPTGWKQKKLSDGQWLYNRSHLIGFQLTGQNNNIKNLMTGTRS
                     LNAPYMLAHENDIAAYIKETNHHVRYRVTPHFEGNELVARGVQLEAESIEDKKIEFNV
                     FIYNVQDGYTINYETGQATKTKN"
     gene            complement(11002..11072)
                     /locus_tag="IXT20_001299"
     tRNA            complement(11002..11072)
                     /locus_tag="IXT20_001299"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11038..11040),aa:Gly,seq:ccc)
     gene            11189..11941
                     /locus_tag="IXT20_001300"
     CDS             11189..11941
                     /locus_tag="IXT20_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HAQ4859755.1"
                     /translation="MTGKELDLLLDTCLLAGKIMMESNAEMYRVEDTMSRIALASGNF
                     RLVSYVTQTGLFIGLDRTSTIRMEQITNRSINLEKVVKVNDLSRKYVASELTLEELYC
                     ELQAIDRDRSFFPVWLQIVSAAVISGTIMVLFGGKMTDLPVTLVIGGLGYAAYLYSLK
                     FFRIKFLSEFLSSLLIGIAAIYSVRFGLGSNQDLIIIGCVMPLVPGVQITNAIRDLLA
                     GHYVSGVSRGTEAMMTATMIGFAIAFIFQLFY"
     gene            11957..12436
                     /locus_tag="IXT20_001301"
     CDS             11957..12436
                     /locus_tag="IXT20_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter"
                     /protein_id="HAQ4859756.1"
                     /translation="MGTIFIQFSFSFLATAAFAVITNVPRRSLICCGLSGAFGWMIYW
                     ICVQIGGSAAFGSLLGSLGVAFISDLFSKRLKMPVTIFNIPGMVPLVPGGLAYQAVRN
                     LVTESYREAAHYAVQAIMIAGAIALGLVLSEVFNHNIRNFKVKRDAIMMKRKKRLFK"
     gene            complement(12687..14267)
                     /locus_tag="IXT20_001302"
     CDS             complement(12687..14267)
                     /locus_tag="IXT20_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HAQ4859757.1"
                     /translation="MYKRQLLELIRVNLLYVNPQLTNKAREKGKKGKRLTIYLLSQNL
                     FSGLIFLLIYGFAMFAMNFVKLPGFFTYYVALFGMLGLTQSISVIYNIFFEGNDLQVF
                     LPLPFGQEQVFSAKILVVALTIIPFVFPLLVMFILTGWRADLGILLTIIISILLFSLF
                     LIILFCLASLIVFGLARTAVFKKHRKTVSSILLGSSMLIAFAGIMWMNHQTGTIENEL
                     TDRHPISILLPFYDAASQPLSQQGLLGWGILAITALGFFLAVKQLIVPKLTEQLTDSN
                     SEFKIKRTHKKNQNVHQLLFSYNAQLVKEPNLIMQVLSSSLLTPIIFILAFALGGEIK
                     LTDLDNKLIGVVFLVGIALAFLTVNQTSFISNLISLDQENFLFIRSLPISMNQYLKEK
                     FRFGWILQSFLTGGIALLSGLSFQLPLFFIPSLLFGSLFGCYLLSLRYFARDYRLLLL
                     NWTNINQLFNRGSGSLGLVATMMGSLIISIILIVLYGMLALFYPFWLVNIPVIGLVLI
                     LSALWIVYYQRTFWKKFE"
     gene            complement(14260..15003)
                     /locus_tag="IXT20_001303"
     CDS             complement(14260..15003)
                     /locus_tag="IXT20_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ4859758.1"
                     /translation="MIEFINVSKSYGKKQALKDLSLSIRQGEIFGFLGHNGAGKSTTI
                     KSLVSIIEPSSGTILVDGMKLTENRLSIKQKIGYVPDSPDIFLQLTAGEYWDLISAAY
                     ELDTQKKEKRLAELTALFDMYSHQNETIASFSHGMRQKTILIGTLLPDPDIWVLDEPL
                     QGLDPQAAFDLKEMMKAHAAKGKTVIFSTHVLDTAQQLCDKLAILKKGELIYQGAVSE
                     LLNGSPDETLEKIYLKMAGRQATGDGPYV"
     gene            complement(15179..15955)
                     /locus_tag="IXT20_001304"
     CDS             complement(15179..15955)
                     /locus_tag="IXT20_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ4859759.1"
                     /translation="MVTRIFILDDSLVFEKMLVEILEESRILLSPWKFEIVQGLNVIQ
                     FVEFIKNNEIYSSDIFFLDIDLNAYYSGIELGEMLREKNEKCKIVYLTSHPNKAITVI
                     NRKITPSGYLIKEFTYEKNKKSIQSYLLKEKENYPVQHATKKRWTTVRYGNSDHYIHY
                     DEILYLMSMPGYKNKIGLVLKDEEILVNGTIKKYKKSLEYEYLCKDLKAYIINTENIT
                     SISRVNEIVTFANGEQLEMGKRSIDKLKNFMQEKIENESF"
     gene            complement(16203..17039)
                     /locus_tag="IXT20_001305"
     CDS             complement(16203..17039)
                     /locus_tag="IXT20_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03943 family protein"
                     /protein_id="HAQ4859760.1"
                     /translation="MLRFLILSGYFELMMYLQVSGKLDQYINVHYRYLAILSMILSAL
                     LALVQLYLWVKNGKAEEKAHEHDHDHGLSKPYQRGIAYVLLALPVIVGTLFPTVSLDT
                     TIVEAKGFNFPVSKESVGDPEMQTQYLKPDTSIYFNKSDYLDQMNELKEKYGDKQTIK
                     ITDENYLEVMELIYNYPSEFIGKKISYEGFVYQTPNDSNTDIFLFRFGIIHCVADSGV
                     FGLLTHMPEGVTVKNDEWYKIEGTIQSDYYQPFKREIPSVVVTNAEKVSAPKNQYVYR
                     SF"
     gene            complement(17050..17979)
                     /locus_tag="IXT20_001306"
     CDS             complement(17050..17979)
                     /locus_tag="IXT20_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HAQ4859761.1"
                     /translation="MFSFIPNSVLQMSTIFLSIVIEALPFVLLGCLISGALQVFLTPE
                     RVKRWLPENRIFSIFTGSILGFFFPSCECGIVPIVHQFVKKGVPVHTAFAFMLTAPII
                     NPIVIFSTFIAFGNSWQMASWRVIGSFFVALVIGVWLAYFQKDAVLKEKITSKVAENH
                     HHHVHEEEQSEKNEPKNQLIHQTGHVLTHSIDEFFDTGRYLIIGGLIAAAMQTYLPTR
                     IMLTLGSTKILAIIIMLLLAFTMSLCSEADAFIGSSLLSLFGTAPVAAFLVFGPMVDI
                     KNLLMMKRYFTGRFILSLIGLIALSVIGFTLFI"
     gene            18171..18524
                     /locus_tag="IXT20_001307"
     CDS             18171..18524
                     /locus_tag="IXT20_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HAQ4859762.1"
                     /translation="MKQTTNLTEVQDILQQSSVAVIYLSMPNCSVCHAVRPRLEELLT
                     HYDIPALHLDAFETPEVASKFEVLTAPVVLIFHQGKEVFRQARFIDFKKIRYLLDELT
                     AEDDSLSYEEIFRTK"
     gene            complement(18598..19254)
                     /locus_tag="IXT20_001308"
     CDS             complement(18598..19254)
                     /locus_tag="IXT20_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HAQ4859763.1"
                     /translation="MKDHIIPKATAKRLPLYYRYLRILNNAGKKKVSSTELSEAVQVD
                     SATIRRDFSYFGELGKRGYGYDVEDLMHFFAKTLNDDQLTNVALIGVGNLGSALLKYK
                     FHQSNSIRISCAFDVNEEIVGRIVDGIPVYPMEDMSEQIKIQQIEVAILTIPAERAQE
                     VVNQLTEAGIKGILNFTAARLNAPSDVLIQNVDLTNELQTLIYFLHSDANAHSENTAE
                     "
     gene            19480..21414
                     /locus_tag="IXT20_001309"
     CDS             19480..21414
                     /locus_tag="IXT20_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAQ4859764.1"
                     /translation="MILLQANQIARHFGAEVLFENIHLEISTGARVALVGRNGAGKST
                     LLKIIAGIEAPDEGKIAKNKTATLGYLAQDTGLDSNETVWNEMLKAFEQVRQMEERMR
                     ELEIFISEGTPDTPAYSSYLKEYDRLQHDFSDRNGYGYENEIRSVLHGFKFDSSFYDQ
                     KIDTLSGGQRTRLALARMLLQHPDILILDEPTNHLDIETLSWLENYLQSYTGALLIVS
                     HDRYFLDKVVNEVYELSRRKMRHYKGNYSKYLDLKAEQLASEWKAYEKQQTEIHKLED
                     FVARNLVRASTTKRAQSRRKTLEKMDRLDRPQGDEKQANFLFSIEKPSGNVVLQIEDA
                     AIGYDNVTLSEPINLDIRRQEAIALVGPNGIGKSTLLKSIIHQIPFIRGKETLGTNVT
                     IGYYDQGQADLHTNKTILAELWDEHPTTPEKEIRSALGAFLFSGEDVEKPIPLLSGGE
                     KARVALAKLAMNKENFLILDEPTNHLDIDNKEVLENALIDYDGTLLFVSHDRYFINRI
                     ATKVVELSENGSKVYLGDYDYYLEKKQEEEEIAELLAQDEPESAPAPTNKSDYYQSKE
                     QQKLIRSLQRKITQVEEEMARIDEWIDQLEQEMVAPENLEDHVKLNELNQDLEAARQD
                     QETKLTEWEELSLELEEYEN"
     gene            complement(21500..22723)
                     /locus_tag="IXT20_001310"
     CDS             complement(21500..22723)
                     /locus_tag="IXT20_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronyl hydrolase"
                     /protein_id="HAQ4859765.1"
                     /translation="MLKAGGEMLDKVMFEEIIHPEKWINECYKELMEKEWNNEIERCR
                     RHTERLIKRISNSQYQFPSECPIKGRYPLRDNIGWTEGFWTGINWLLFEKTGDPLFQQ
                     TAEKADIDFIHRIDCQIITNHHDLGFLYSLSTVADYKITGNRKMKEASIDAAETLLQR
                     FWEKAGVIQAWGDPSDPSQQGRMIMDCCMNLPLLYWASEETGNQKYKKAAQAHIQNAA
                     DYLVREDGSTYHTFYMDVKDGKPRYGSTHQGYSDNSCWARGQAWGIYGFALSYRYLKE
                     QKYFDLSKILANYFMNRLPEDTICYWDLIFDDSSREEKDTSSMVIMVCGLLELIESGL
                     LDKTEKNLYQSAVWKIMRQLKERYATTEEEDGFLTQSVYNKPKNNGVNTPCIWGDYYY
                     LEALVRMTSNWSSYW"
     gene            complement(22717..24615)
                     /locus_tag="IXT20_001311"
     CDS             complement(22717..24615)
                     /locus_tag="IXT20_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2264 domain-containing protein"
                     /protein_id="HAQ4859766.1"
                     /translation="MVVKRGENTMKHPVPLLRTRQDYFDLFKKINQPLQPFYRASGAQ
                     IDYIHQGVGYGNKIAGMEGFSRVLWGAGPAIDQLDERWLKLILSGIKAGTDPSHPDYW
                     GEIHERDQRMVEMPAMLLALYHHEQLLWKKLDTGEKSQIIHWFSQIFEYECADGNWQF
                     FKIIVGTIIQKFGYPVKESALKEAFDNIENCYLQNGWYRDSSRGRQDYYNPFAFHYYG
                     LIYSVLEPEKPISQIYKNRAIDFSRDYIHFFAEDGANVPFGRSMIYRFAVSSFWSAML
                     WADLLPFKLGEIKGLVNRNIRWWMQKEIFDEKGILNIGYTYPQLTLTEPYNSTLSPLW
                     LNKIFLLLALPEDHEYWTAKEMPMPIREGTKLLSEANLLIQHDNGHTFFLNAGQLGPN
                     FHTLTNEKYLKFAYSSQFGFSIPRANQLKAEMAMDSMLGIQRADTYITTSFKNESIKT
                     YGQFIVRNNVRDIVCKKEVLASTWEPTLQAKIRTWLIPFEGWQIRVHKVELDTEYVLY
                     ETGFAIECSPEKEGIIIEEDKENYRVSEAGFSGIICLSDDTIKRKSTAIMGLPNTNLM
                     TWENTFIPGLEGTFGKGTHWLGTGVVAHQDRDYSLEVWNNRPKIDFDGTTGLTIQNKN
                     NKKRLKLC"
     gene            complement(24609..25727)
                     /locus_tag="IXT20_001312"
     CDS             complement(24609..25727)
                     /locus_tag="IXT20_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 88"
                     /protein_id="HAQ4859767.1"
                     /translation="MNEQTKEWSRKILNVASEKIDAELDRMGNKIPYISYNGKYTDVI
                     KDRGIDWWTNGFWSGILWQLFHKTKREKFKEAAIQQEQLLESALYHFSDVHHDVGFLF
                     LPSAIAHYQQSQNKHSRQLGLHAATVLAGRYNISGNYLVAWNDHKPGWMIIDSMMNLP
                     LLYWASRETGDPRFSKIARQHADTTAKYLVRKDGSTGHIAEFDAESGKYIRLLGGQGY
                     NENSCWSRGNAWALYGFALSYRYTKNPYYLAIAEKIAVNFIQKIEKTGYIPLIDFAVK
                     DSNIDTSAGVCAACGLLELAGYVSSVRSKMYHEAAINILQKTEKHYANWNMDEDGIIG
                     GGAEAYHRELTYNVPLIYSDYFFIEGLLRLEQSELAIW"
     gene            complement(25810..26643)
                     /locus_tag="IXT20_001313"
     CDS             complement(25810..26643)
                     /locus_tag="IXT20_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAQ4859768.1"
                     /translation="MTKQKIQSRIFTYVIIVFGALIFLMPYLYMIVASTQSNQEILSG
                     GINFKFGDMLLKNWQAIMARYNYPLIIWNSIVIAVISTVSATASSTMAGYALAKYRFM
                     GNKLLFNTVMLSRMVPFFTTLIPLFYIMSRLGLSNTYAGVIIPSIASTTSVFMMRQYS
                     SQFPTALMEAARIDGANEWVIFTKIAVPILSPTIITSGLLIFMGSWNQYLFPLVMLSD
                     KDKYTVPLIIKNMTVSSTGEPIKYGAIMLILATSVIPMVLIYVWAQSKFKENDIGAAN
                     K"
     gene            complement(26636..27553)
                     /locus_tag="IXT20_001314"
     CDS             complement(26636..27553)
                     /locus_tag="IXT20_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ4859769.1"
                     /translation="MMQSSLPSKTIKNSKKKATDTSHKKSAYGLVTPFVIFFIVTILI
                     PIIISFAFSFMKIGYSFEFVGFDNYLAIFRDPLFIKSYTNVLIIMAGSIPITMLLAIY
                     FAVLLNSAQLKGKGFFRTVYYIPTITSTVAVASVFMTFFNPTGLFNGFLNALGINGVQ
                     WLTNPFWIRISMIITMIWLSVGYNTILFLAGLQGISKEIYESAEIDGASKFRQFFLLT
                     IPMLKPIILMATVLATINGLGNFNIPNIFFGTSNGPENSALVVGVNLYKTSFEMVDFG
                     KASAIAWTMVFVSVILSAIQFKFGGDKND"
     gene            complement(27780..28904)
                     /locus_tag="IXT20_001315"
     CDS             complement(27780..28904)
                     /locus_tag="IXT20_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAQ4859770.1"
                     /translation="MKKRYKLVIALLNITLLFSLSACGKTNEEVTANIADPMIKKATT
                     LEVYVANGKLTNYVENAAKIYNRKTGANIDLKIVNVASGTATVQMITPKLVTHEDMPD
                     IISMADTSAAGVLEKFQDAFYSADEYGFYDKYGDQFYDQKLNILRQQTSNETIIPWAS
                     DFTPALCYYQPKKFAEIGVDFEEISSWEEYIQVAKALKEKTGSFGIALPESGDQELFI
                     NMLAQQNQSLLDEEGNIRLNTKEAKHAAKLIKEMIDSDIVHFYGAQDAEKAFQESAMF
                     VAGGWYATNMSLNFPDASDKWRMAPLIPFSKENPGKGAVSGGSSFYVPKDAKNPQVAQ
                     QFLTFMLTDEECLANALELGVATSNSLAYQTEAAEKKFDY"
     gene            29087..29995
                     /locus_tag="IXT20_001316"
     CDS             29087..29995
                     /locus_tag="IXT20_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ4859771.1"
                     /translation="MVLLLTNNQSSNLVKKTLTYSIDTHLHLSAAPYFRLLINESANL
                     YAEFAHKSIELDNPSLLFIDKQQEVQIFSTQKETQTFIIIQFYFDEQLLAHITGQSFS
                     IDQEITSIITFLKNEQFKIDYLKHSKPAISYDQEINSFFILCQQMSIHLLSLLVTYCL
                     QKIQLSSLPRHYQQELALLTIPSNEKVIPKKKHILYSDELVDPIIRYLEEHLSENFLL
                     EQLAQIFFISPASLKKHFRKVNGKSVISYFKELKMEKAKRLVLENELTYTEIADILGF
                     HSIHHFSSAFKKYTGLSPSKYYLSLK"
     gene            30136..31749
                     /locus_tag="IXT20_001317"
     CDS             30136..31749
                     /locus_tag="IXT20_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-disulfide reductase"
                     /protein_id="HAQ4859772.1"
                     /translation="MKVVVIGGVAGGPSFATRFRRLNEAHEIIIYERGENISYASCAL
                     PYYLGGVITDRDSLIERTPEILKTKNNIDVFTKHEVTAIDPSTKRLTVKDLSTNEEIK
                     TDYDKLIISSGARPDYPDIPGVFEAENGFVLRSVTDADRIKSFLEEKNPQHVVILGAG
                     VMGLELAENFKHRGLDVTLIDQLPQVAFPYDPEIANLVYDKLLKEGLAVHLETRVTEI
                     RDKGREIVLSDGSVLSADMLIFAVGVSPKNEVVKATGIQLSDTGQIIVDDQLQTNLPD
                     IYAIGDIIETTSVVTGQPIQSMLSSAANRQGHMLADILNGTPMRYRGYIGAGVAKIFD
                     HTASYAGMTEHELKASGITNYKTVFITPFDHAYFYPEATRLNLKLIFDADSGRILGGQ
                     AFGEKGVDKRMGELSVAITGNLTVFDLPDLELPYSPPYSTTRDPLNIAGYVAINQMTN
                     IVETIKASDIPENDLKEAFFLDIREPNKAPSGSISATKNIPMNELRDRINEIPKDKKV
                     YITFRRGLNTYTSARILAGLGIKAILIEE"
BASE COUNT         9975 a         6413 c         5501 g        10409 t
ORIGIN      
        1 tatgaaaaaa tatttcaatt ttattttttc gtttcaaatt attctattag tgacatcctt
       61 gaactataaa aataggagta cggcatcatc tgccgcactc ctattttttt cgtcatttgt
      121 ttcttaaata aaacgattct cacgcaattt tatttttttg atatcttcta ctgtttgtgt
      181 tccagctaat tgcatgacca tttccaattc tgttttaaag aaatcaaaca cttgatggac
      241 tcctgtactt cctcctaaag acaatccata aataacgggt ctgccaatag caacaagatc
      301 tgcacctgat gcgattgctt tgaaaacatg ctgtccgcgt cgtacaccgc tgtcaaacac
      361 gattggtaca cgtttatcta ctgcttctgc cacatattgc aacgaatcaa acgctgctgg
      421 accaccatct agttgacggc caccatgatt agatacccag attccgccag caccagattc
      481 taatgaacga tagacatctt cttctgactg gacacctttc acatagactg gcaaatcaga
      541 atgagcagca ataaattcta catcttttgg actaagcttc tgtttggaag atttgtacac
      601 agcatccatc gtttggccta ctcctgattg ataagcctga acgatcggca ttggcagtgg
      661 gaaggtaaac ccatttctgc ggtctgtttc acggtttccg ccaactgttg catccgctgt
      721 caatacgatt gcttttgctc cattacgctt ggccacttca agaatatcca aattgatccc
      781 atcatctttg ctcatataaa attgaaacca ttgcggtgct ttctccccgc ctgcttctct
      841 tatctcttct aatgtacaag aagcgtaaga acttgctgta tagatcgtcc caaaatctgc
      901 tacacctttt gcagaagctt tctccgcctt cacatgagct aagccatgag cagcaactgg
      961 tgccatgatg attggtgcag tcagcgtttc tttgtcaaaa tgtgtagttg tatcgggtaa
     1021 ttctacatct cttaatacat gaggaataat cagctgatga ttgaatgccc gttcattttc
     1081 ttgataagta aataagtcgc cagcaccact gctgatgtaa ccataaccac cagctggaat
     1141 cacttgtgaa gcggctattt ctaaatcttc catattgata aaatccacta ttccttcatt
     1201 tgtacctgct tgatacgttt tttccatttc aatgccttcc ctctataaaa aattgaatga
     1261 ataatatgat agcgtttaca cactttatat tactcttccg tattgataca ggcaaataat
     1321 ctgtttacat ttcagtcatt ccagttgtta ctactctgaa agaaatgttt agaacacaaa
     1381 tagataaaaa acgatgggat tttgacaaaa gtcgtgtcaa agtccctttt acgaaatact
     1441 gtggatgcct attgaaataa gtgcgggttg tttctttgta tttcaatcgt ttcttgccat
     1501 tcttcgttta attttggatt accgttttct tccttgaacg cataaggtcc caatccaaaa
     1561 agctcagtta gtatttgatt cacgattcca tatagctcgg tgtgatcgga cacatgatta
     1621 ttgaagatca tctcattatc accacgtttg aatctcacat tcgggctctc atgatgaacc
     1681 tctatgcagt tttccccttt ttttaactga cggatttctg aaaacatctc ataaatatct
     1741 tctccaataa aaccttcagc aagcgccaga ctgtccacaa gaccgcctac tgtcaactca
     1801 gagattatcg ctttgttggc accaagcttt aatgtatagc ttgttgcttc tactacgccg
     1861 catgcattgc aaccaccttc tgatagttcg ctttttctga tcaattgaac ttctttcatt
     1921 ttctgttcct cctcttagtt ttctacttat gtatttttta tcacctgacg taagtgtatt
     1981 ttatcgtcac gatcacgtga tttattcttc agagattcta cctgaataat tcatactttt
     2041 aattaaaacc atgtgaaact gcctcacggc agcttacaat tactttttca tcttcacccg
     2101 ttaagtgatg aaaaaagaac ccctttctgc tatggttaaa gtgactaaac caaccaaaag
     2161 aaaggagttc ttttcatgac tagcttacac aaaaaccaag taaaattcaa ttcaaatatc
     2221 actatttctc atacaggtgg tcaattatcg agtgattcgg gtctcgtcct agtgaaagag
     2281 ctgatgaaca tctttggttt ttctgaactg gctaagcaac acattcacat tgaagacgaa
     2341 cgagcgtatt ttactcatga caacttatcc atacttgagc agttcattat gcaattaatt
     2401 gctggttact cagctgattc tgcagctaat ctcctgagac aagatcctgt gtttaaagct
     2461 gttttagata ggaaagaact cgcttctcaa tcttcacttt cccgattttt agatcgacta
     2521 tctgaggaga atatccatga acttcaagct ttgaaccaag aacttatcga taaagcacgc
     2581 ctcatccgta atgatacgga attaatcatt gatttagatt cgacccattc agatacattt
     2641 ggtcaccaag aacaaacgaa ttataataca cactaccaaa cctatggtta ccatccattg
     2701 gtagcttttg acggattgac tggtgacttc ttaaaagctg aactacgttc aggaaatcaa
     2761 tatacatcaa aaggcgtaaa ggagtttctc acacctttat tagagcacta taatcactct
     2821 ctaccaaaca ctgacatctt ggttcgtgga gacagcgggt tcgctacacc tggtgtgtat
     2881 gattcgtgtg aatcaaaaaa gagtcattat gttattcgac tgaagaataa tcgtagacta
     2941 ggtcaaatag ctgagaaatc agtactttat ggcgataatc aaaagtggga agaacgagaa
     3001 gttcagtact tctcgaccac ttatcaagca caatcctggt cacaaagtcg tcgcgtgtgt
     3061 atacgctcaa cacgtgaagc gggcgaacta ctctttcgac atgaatttat cgtgacgaat
     3121 ctatcagaaa atgtttctcc tgataccatc ttttctatct atgccaaacg tggcacaatg
     3181 gagaacttca ttaaagaagc gaaagctggc ttttactttg acaaaacaga cagtcctcgc
     3241 tttttggaga atcatgtccg aatgatgctc agcttgatag cttacaactt agtcaacttc
     3301 ttaagaacga ttggctttga agaagtccaa aagggaatga ccattcattc tattcgattg
     3361 aagtttctga aagttgctgg gaaattagtc caaacgggta gacgagtcta tctcaaatta
     3421 tctagttatc atgtgtatca gaatgaattc tacagggtct ttgctcgcct gaggcgagcc
     3481 agtcaatgga tctaatccaa caatctaacg atttttttac tgggaagtta tccagggaga
     3541 agtatgctca aaatgcctta cacccaaaat ttattcctca atatttttga acagtgaatg
     3601 tttgaagtaa ctttttagtc aaaatcagtt gatagggata ttagtgatac atatttcaat
     3661 aaaatgtgcc aaagaattaa agctatgaat tattcaggat tctatttatc tatctaacat
     3721 atcctttatt cttctacttt tctttcttta gcatataaga gatacgcata gtaatcaagc
     3781 aaggattgtt gatataaaaa gaacaaatac cgctattttg atttcttttg actctattac
     3841 tcatataaat aaaatttatt atacctgctc tttttgacta tatcctcatc tgactcctat
     3901 acttcgtttg tattaaaact taggaggaat cattcatgct tacagggcta aacttattaa
     3961 ataatccttt tctaaataaa ggaacagctt ttacaaaaga agaacgggca aaatacggaa
     4021 tcactggtat gcttccaagt actgtccaaa cattggaaca acaatctgtc caagcttacg
     4081 gtcaatactt aagcaaacag accgatttgg aaaaacgtat cttcttaatg aatctattca
     4141 atacgaaccg gacattgttt tataaattga tgggcgaaca tttagttgaa ttcatgcctg
     4201 tcgtttatga tcctgtcgtt gcggattcaa tcgaacaata caacgaaatt ttcttgaaac
     4261 cacaagatgc tgtatttctt tcaatcgacg aaccagaaaa tatcaaagca agtttgaaaa
     4321 acgcagcaga tggacgcgac attcgtttga tcgttgtgac agatgcagaa ggaatcttag
     4381 gtatgggtga ctggggtgtg aacggtgttg acatcgctat cggtaaattg atggtctaca
     4441 ctgccgcggc tggtatcaat cctgcacaag tcttgcctgt atcaatcgac gctggtacta
     4501 ataacgaaga attattgaat aatccattat acttagggaa tcgtcatgct cgtgtagaag
     4561 gcgaaactta ctacgaattc attgatcaat tcgttcaatc agctactgaa ttattcccag
     4621 aattgttatt acattgggaa gattttggac gaggaaatgc tgcaacgatc ttagaaaaat
     4681 acgaagataa aatcactact ttcaatgatg atatccaagg cacaggaatc gttgtactag
     4741 ctggtgtatt aggtggattg aatatatcag gtgagcaatt gaaagatcaa acgatcttga
     4801 catttggtgc tggtactgct ggtgtaggta tcgcaaacat ccttttagat gaaatgatcc
     4861 gtcaaggtgt acctgaagaa gaagcacgcc aacatttcta tcaagtagat aaacagggtt
     4921 tattgtttga agatacaaaa ggattgactc ctggtcaaat cccattcgct cgtaaacgct
     4981 cagaattcac taatgctgat gaattgacaa acttagaagc agttgtaaaa gcaatccatc
     5041 caacgatcat gatcggtact tctacccaac ctggagcatt cactgaagaa atcgtgaaag
     5101 aaatggcggc tcatacccca cgtccaatca tcatgccttt gtctaatcca acaaaattgg
     5161 cagaagcaaa agcaaaagat ttgattgaat ggacggacgg aaaagcttta gttggaacag
     5221 gtatccctgc tgctgatgtg gaatataacg gtgtaactta ccaaatcggt caagcaaata
     5281 atgcgttgat gtacccaggt ttagggcttg gtttgattgc ttcaacagct acacgagtaa
     5341 atgctgaaat catttctcaa gctagtcgag cattaggcgg aatcgttgat gtaacaaaac
     5401 caggagcagc tatcttgcct ccagtagcaa aaatcactga attctctcaa acgattgctg
     5461 aaactgtcgc aaaatcagtt gttgcacaaa aattgaatcg tgaagaaatc acggatatca
     5521 aagaagctgt tgaatctgct aaatgggttc ctgaatataa atctttagaa gattaagatt
     5581 gttatagtaa aagaacatac agaaactttg tatgacgtct aaaaagaaaa aagcgactgc
     5641 ggcaaaacag cagccagcca actactgctc ttttgccgct tctctttggc ttttttcata
     5701 cgatacagaa taagaaagga tctgtaaaaa tggcacaaaa aacaacaacc gcaactgcag
     5761 ttgaggaaaa gaaaaaagaa gggttttggt caacaaaaat taccggcgta agtttgcccc
     5821 tttatttgat catggtcctt gtcctgatca ttgcaatggc tttaggaaaa ttaccgacga
     5881 atatgattgg cccaatcgct gttttggtca ttttcggtaa tttatttcat tttatcggaa
     5941 caaaaatccc tatagtcaaa agttatttag gtggtggctc cgtttttgcg atctttgcct
     6001 ctgccgcgat tgctacattt gggatcttac ctgagtatgt agtaaaatcc acagaaaact
     6061 ttgtcagcaa tatgggcttt ttagactttt atattgctgc cttgattact ggtagcatcc
     6121 ttggtatgaa ccgtaatcta ttgatgaaag cttctgttcg ttttattccc gttgcactta
     6181 tttcaatggt cgtctccttt tttgccgtag gactcgtagg tatgcttatc ggaaatggtt
     6241 ttgcaaattc cgttttatat atctcattgc ccgcaatggc tggtggtgtt ggagccggag
     6301 ctgtaccttt atcaacgatt tatgcaaatg ttttaggtac ggacgcttct tctatcattt
     6361 cccgcctgat tcctgcgaca gcaatgacaa atgtcctagc tatcattggt gcagcgttag
     6421 ttgctcgtgc gggacaatca atgccaaaat tcaacggtaa tggtaaatta atggaaaaag
     6481 gcgaccttgg cgatggaaaa cctagaaatg taaaaccttc cattccacaa ttaggtgtcg
     6541 gtttgatggt atcattgact ttcttcatct taggacaaat ctgtaactat tttgttccta
     6601 aagtccatgc ttacgcattc atgatcatta tcgttgttat ctgtaaaatc gcaaacctat
     6661 taccagaata ttacgaagat gcagctatca tgttcaacaa cttgattgtc aaaaacttga
     6721 cagcagctgt tcttgccggt atcggtattg cgttattgaa tctaaatgta ttggcttctg
     6781 ctttgacatg gcaatttgtt gtcttatgtt tgacaagtgt tatcgcgatt tcagtagttt
     6841 caggatttgt cggtcgtttg tttggtttat atccaatcga atcaactatt actgctggct
     6901 tatgcaacaa ctcgatgggt ggtacaggaa atgtagctgt cttatctgca gctaatcgga
     6961 tggaacttat tgcttttgct caaatgggca atcggcttgg tggtgccatc gttttgatca
     7021 tctcaggttt cttgatgcaa ttactttctt agtcaaaaaa aatacctgat catcaagtat
     7081 cggcttgatg accaggtatt ttttgataaa aatttacagg attttcaaaa atcttttttg
     7141 cattttatcg gaatgataac cgatgcgttc ataaaatcgg tgtgcttcta ctctgtattc
     7201 tgcagaatta agtcttattg ctgatattcc tcgtcttttc gcttcttcta cgccagtcat
     7261 caaggccttt ccgattcctc ttttttcagc atgtttagac acagcaagtg ccataatatt
     7321 gaacatcgga tcgctgaaaa tcgattcata ggactccgct tgtacatagc cagctgcttt
     7381 tttagaagta ggatcttcat agaccaagaa aaaatttttt tctggctcct tcaacagttt
     7441 cttcatttgt ctcaccgtaa cggttaatgg tacatcatat cctaattgtt ccgcattgat
     7501 ttctcgtagt gcttctgcat ctcctaaacg catttcccgc aacatccaaa tcaccagcca
     7561 atttttattt ttttaaatga atttttttat cacagtatac tccttttttc acttaaaaaa
     7621 agacttttca gtgattttga cttgcttgaa tccagatcct tctaatgttt gcaagatgac
     7681 gaaagcattg taaagattga tcaaaagaac cacgatgaca atcgcttgaa aaataaaggt
     7741 cacttttttt ggagacaaaa ccctgctaaa tcttgctccc cacaaacctc caataacagc
     7801 agcggggata acataaaaca acatagataa atcaaagcgc ataaagctac ttgttaaagc
     7861 aatcgttacc aattttgcca gttgagaaaa gaaaatcgta gatagagaat atagtgttgc
     7921 ttcttttatt ggcaatgcaa acatcagcat caacaaagaa acattgactg gtccaccacc
     7981 aatacctaaa aaactagcta aaaagcccag aaccccccca catataaggt accaagtcca
     8041 tgacgtcaaa tgaaattttg gctgatgatg cttggtgtag aagaacgcaa aaaccaaggt
     8101 caataccgtc aaaaaaattt ggatcagctg cacatgcttc tcgttttcaa ataactgtaa
     8161 aaaaacttcg aatacaatgt tgccgactac tcctccaaca acagctccac tagataccca
     8221 accgacaatc tgccaattca gtgatttccc gcttgatact tgtcgcacag tcgaaacaat
     8281 ggacatcgtg aataccgcaa ctgttgagta aaaagaaatc cctgcaacag aatgggcgcc
     8341 gataagatca agaatcggtt tgatcaacac gcctccaccc atcccggaaa tagcgccaac
     8401 tgtgttcgcc agaacgatca ccacaaaata aatcagacca atcatcttgt atttcctttc
     8461 tcttgtttat atcttttagc gattgttact cttttctttt attatacagt aaaagaaaca
     8521 aacgaggctt cgaaagcttt tctacttttt aaggaaattt agtgctaaac gaaaaaaccg
     8581 ggctctactt ttcagagagt ccagcttttc ttacacctta tcgattcttt tgttctattc
     8641 ttcttccgcc atgaatgtat tgaatacttc ttcgatcata tcccattctg cgtctgtttc
     8701 gatttgttct aactcgcctt ctgttccatc ttcgttttcg atgtatgcat aagcttgtaa
     8761 ttctacatct tcatcttctg aaacaccagc tggatataaa agaacatagt tacgtccaaa
     8821 ttcttcttgt ccgtcaactg ttagcaagat ttcgtataat gtttcatttc cttggtcatc
     8881 caccaatgtg atgtgttcgt gtccttcgtg ttcgtgggaa tggttatgtt ctgtcataag
     8941 attacctctt ttctttttat tattaaaata attgcttacc tgagccgtct aaataatttt
     9001 gtaaaatcat cacagctgct aatttatcga tgactttttt tcgtttcgct cttgaagtat
     9061 ttgcctgctc tactagcata cgttctgcct gaacagtggt tagtcgttca tcttgataat
     9121 caaccggcaa ggagaatagt tctttgatct tctctccata tgacatggaa gcttctgccc
     9181 ttggtccgat ggaattgttc atattcttag gtaatccaac tacaaaacgg tctacttcgt
     9241 actgcgccac taattccttc aaccgatcaa aaccaaactc ttcttctgcc tcattgattc
     9301 gaatgatttc tactccttga gctgtccagc caaagggatc gctcacagca acgcctactg
     9361 tcttagaacc gacatccaag cccattactc tcataaattc actccatgat ttgagaggta
     9421 gtctttgaca attacttcca tgatttcatc gcgttcgtga cggcgaatca aatttcgtgc
     9481 atcacgataa cgaggaatgt aggccgggtc tccagaaagc aagtagccga caatctgatt
     9541 gattggattg tagccttttt cttccaaagc ccgataaaca atcgctaatg tttcgctcac
     9601 ttcttttttg cgactatcgt caaaaccaaa acgtacggtt tcatctgtaa aacccatgat
     9661 tctacacctc tttcccgcaa ggtaattcca gcttaccttc tacaccctta ttttactcga
     9721 agaaagggaa aactacaaac aaatattcca aacttctgaa taaattaaga aacacgattt
     9781 gtctgatttt tccttttttc tgtggctttt tcaaaagccc tctttccata aagtatacgc
     9841 tatatttctt tgaaagtatg tttttacaca attttttcat aaagttcata aaaaattcga
     9901 attgctgcta ctaatttttc gttttcgttg cttgccctgt ctcataattg atggtgtagc
     9961 catcttgaac attgtagata aagacattga attcaatttt tttgtcttca atgctttctg
    10021 cttccaactg cacacctctt gctaccagtt catttccctc gaaatgagga gtgacacgat
    10081 agcgtacatg atgatttgtt tccttgatat atgccgcaat atcattttcg tgggcaagca
    10141 tgtacggagc attcaacgaa cgtgtccctg tcattaagtt tttaatgtta ttattttgac
    10201 cagtcaactg gaaaccaatc aaatggctgc ggttgtataa ccattgtccg tcacttaatt
    10261 tcttttgttt ccaacctgtc ggatcaatat acaaaggttc tctcttttca gtaggaaaca
    10321 agtcttttcc caacatcgca ttggcttgtc ctactctgtt caatttgtct atatttgaat
    10381 agctttccca tgtccctttg gaaagactaa gatcctcttt tgtaaactcc ggctgattcc
    10441 cattgatttc tatttcttga tgttttccat catattctgg cacatccgct acttgagaag
    10501 cttgcggtgt ggaatcaaaa atggttgtaa tttgttggaa gatatctgaa tctttttgtg
    10561 tggtggaaat accgagtgcc cctgcaatca cagcagcgac gatccccacg atcgccttgg
    10621 ttgttttttt gctcatttct ttactccttt actaacagtt ccctaaatct taacatatgt
    10681 tcccctataa ataaatactg gaaagaaata gaaacagtaa aaaaataaaa ccagttttta
    10741 gacggcttaa gaatacaaaa aaagagacgg attcatcgtc tctactcaat cacaaaacac
    10801 tatttacaag gtatttatta tcattcaaaa aatttttctt aatactcaaa actaaacggc
    10861 ttctgtacga cctcttggta aacagtgttc aaaagcttgc atctgcggta ataagtccaa
    10921 ccaagtcaaa aacataagaa gcttttttgc ttgactattc ttattacttg atgcagggca
    10981 gctttttcac agcctctttt aagcgggtga cgggaatcga acccgcgaca tcagcttggg
    11041 aagctgggat tttaccacta aactacaccc gcgatacaaa aacagtatac gcctctttca
    11101 ctccttcctc aagttttttt attctttacg cttccgatat gttttgtagt aaaataaaac
    11161 taatcaatca attattggag aagatgctat gacgggaaaa gaattagatc tgttgctgga
    11221 cacttgttta ctcgctggga aaatcatgat ggaaagcaat gcggagatgt atcgggtaga
    11281 agatacgatg agtcgaattg ctttagcttc tggaaatttc cgtttggtca gctatgtcac
    11341 tcaaaccggt ctctttattg gtcttgatcg tacctcgacc atccgaatgg aacaaattac
    11401 caaccgttcg attaatttag aaaaagtagt aaaagtaaat gatctttcac gtaaatatgt
    11461 tgcttcagag ctaacattag aagaacttta ctgcgaactg caagcaatcg accgagaccg
    11521 ttctttcttt cctgtctggt tgcaaatcgt tagcgcggca gtgatcagtg gaacaattat
    11581 ggtgttattt ggcggaaaga tgaccgattt acctgtgact ttagtcattg gaggattggg
    11641 atacgcagct tatctatata gtttgaaatt ttttagaatc aaatttttat cggaattttt
    11701 atcttcttta ttgattggca tagccgctat ctacagcgta cgttttggct taggaagcaa
    11761 tcaggatcta atcattatcg gatgtgtgat gcctttagtt cctggagttc aaattacgaa
    11821 cgctatccgt gatttgctag ctggacatta tgtctcagga gtttctcgcg gaacagaagc
    11881 gatgatgact gctacgatga tcggttttgc gattgctttt attttccagc ttttttatta
    11941 ggaaaggaac acaagtatgg ggacgatatt tattcagttt tcttttagtt ttttagcgac
    12001 tgcggcattt gccgttatta caaatgtacc taggcgttca ttgatttgtt gtggtctttc
    12061 tggtgctttt ggctggatga tctactggat ctgtgttcaa ataggcggat ctgctgcttt
    12121 tggttcattg cttggttctt taggagtagc attcatcagc gatcttttct ctaaacgttt
    12181 gaaaatgccc gtaacgattt tcaatattcc cgggatggtt cctctagtcc ctggtggttt
    12241 agcttatcaa gcggttcgaa atctcgttac tgaatcctat cgagaagcgg ctcattatgc
    12301 ggtccaagca attatgattg ctggagcaat cgctttaggt cttgtattat cagaagtctt
    12361 taaccataac atccgtaatt tcaaagtaaa gcgtgacgca atcatgatga aacgaaaaaa
    12421 acggcttttt aaataaacga agtgtaacaa agaaaaggtt gtgaaagaga cgttcagcct
    12481 tgagcgatga caaaatatct cacaaacatt gcgacaagta accgcaggag caataagaag
    12541 taattatgag tattaaaaaa agttttgtaa aaatagcttc tcacattttt acataaaatt
    12601 gacttaatac tgagaaactg acttttgaac accctctatc tgtaaaaaag gggctgcgac
    12661 atgatttttg tcacggcccc tcttctttat tcaaattttt tccaaaacgt tcgttgataa
    12721 taaacgatcc atagagcgga aagtattaga actaaaccga tcacaggaat attcactaac
    12781 caaaatggat aaaacaaagc caacattccg tataagacga taagaataat actgatgatc
    12841 aatgaaccca tcatagttgc gactaaaccc aaacttcctg aacctctgtt aaataattgg
    12901 ttaatattgg tccagttcag cagcagcaac cgataatctc tagcgaaata acgtagtgac
    12961 aataagtagc agccgaataa agaaccaaat agcaagctag ggataaaaaa caaaggcagc
    13021 tgaaaagaca gtccgcttaa taatgcgatc ccaccagtta aaaaactctg caatatccat
    13081 ccgaatctga acttttcttt taaatattgg ttcatagaga ttggcaaaga acggataaac
    13141 agaaaatttt cttgatccaa agaaataaga ttgctgataa aagaagtctg attaactgtt
    13201 aagaaagcca atgcgatccc taccaggaaa acaacaccga ttaacttatt atctaaatca
    13261 gttaatttta tttcgcctcc tagggcaaaa gcaagaataa aaatgattgg agttaacaag
    13321 gaagaagaaa gaacttgcat aatcaaatta ggttctttta ctaattgagc attgtaacta
    13381 aataaaagct gatgcacatt ttggttcttt ttatgggttc tttttatttt gaactcactg
    13441 tttgaatcag tcagttgctc tgtaagcttt ggtactatca gctgttttac agcaaggaaa
    13501 aatcccagag ccgtgatagc tagtattccc caacccaata aaccttgttg agaaagtggc
    13561 tgactggcag catcatagaa tggcaataat atcgaaatcg gatgccgatc tgttaactca
    13621 ttttcaatag tccccgtttg atgattcatc cacatgattc cagcaaatgc gatcagcatc
    13681 gagctgccaa gcaagatact agaaactgtt tttctatgtt ttttaaacac tgctgttcga
    13741 gccaaaccaa aaacaatcaa actagccaga caaaaaagaa taatcaaaaa tagagaaaac
    13801 aaaaggattg agataatgat agtaagcaaa atccctagat cagctcgcca tccggttaaa
    13861 ataaacatga ctagaagcgg aaaaacaaaa ggaatgatgg tcaaagcaac taccaaaatc
    13921 tttgctgaaa aaacctgttc ttgtccaaaa ggcagaggca aaaatacttg caggtcattt
    13981 ccttcaaaaa agatattata aatgactgat atactttgag ttaaacctaa cataccaaaa
    14041 agtgccacgt aataagtaaa gaaacctggc aatttaacaa agttcatagc aaacatagca
    14101 aatccgtaaa tcagtaaaaa aatcagccct gaaaaaagat tttgagacaa aagatagata
    14161 gttaatcgct ttcccttttt tcctttctct cttgctttat tcgtgagctg tggattaaca
    14221 tataaaagat taactcgaat taattctaat aattgtcgct tatacatacg gtccatctcc
    14281 tgtcgcttgc cttccagcca ttttcaaata aatcttttct agcgtttcat caggtgagcc
    14341 attcaataat tcactaactg ccccctgata aatcaattca cctttcttca aaatagccag
    14401 tttatcgcat aactgttgtg cggtatccaa tacatgtgta gaaaaaatga ctgtttttcc
    14461 ttttgcggca tgagccttca tcatttcttt caaatcaaat gctgcttgag gatcaagtcc
    14521 ttgcaaaggt tcatccaata cccaaatatc tggatctggc agcaacgtgc cgatcaaaat
    14581 cgttttttga cgcattccat gtgaaaaact tgcgattgtt tcattttgat gactatacat
    14641 atcaaaaagc gctgtcaatt ctgctaaacg cttttctttt ttttgtgtgt ctaattcata
    14701 ggcagctgaa atcaagtccc aatattctcc agccgtcaat tgtaaaaaaa tatctgggga
    14761 atcaggcaca taaccgattt tttgtttgat actcaatcga ttttccgtca atttcattcc
    14821 atctactaaa atcgttcccg atgatggttc aataatacta actaaacttt taatcgtagt
    14881 tgattttccg gcaccattat gtcctaaaaa tccaaaaatt tccccttgtc ttatcgagag
    14941 agacaagtct tttaatgctt gttttttccc gtaactttta cttacattga tgaattcaat
    15001 catttctgta ctccttttgt ttgttattac tttttattgt acccttcgtt attggtggag
    15061 taaatgtcaa aaagagtgca taacaaaaaa ggatagaaac gactaggctg ctacttgggt
    15121 tgttctcttc tacaggaaaa cactcttcta acaggtaaga tatgagtctt tgttttcttt
    15181 aaaatgattc attttctatc ttttcttgca taaaattctt tagtttatca atacttctct
    15241 ttcccatctc tagttgttct ccatttgcaa atgttactat ttcgtttact ctgctaatag
    15301 atgtaatgtt ttcagtatta atgatataag cttttaaatc tttacataaa tactcatatt
    15361 cgagtgattt tttatatttt ttaattgtgc cgtttacgag aatctcttca tctttcaata
    15421 ctaggcctat tttattttta taacctggca tgctcattag ataaaggatt tcatcgtaat
    15481 ggatatagtg atcgctatta ccataacgga cagttgtcca cctttttttc gtagcatgtt
    15541 gaacaggata gttttctttt tctttcaata agtaactttg gatgcttttt ttattttttt
    15601 cataagtgaa ttcttttatc aaataaccac taggcgtaat ctttcgatta ataacagtta
    15661 ttgctttatt cggatgagac gttagataaa caattttaca cttttcattc ttttctctaa
    15721 gcatttcgcc tagttcgata ccgctataat aggcgttcaa atcgatgtct aggaaaaaaa
    15781 tatctgagga atagatttca ttatttttaa tgaattcaac aaattgtata acgttcaatc
    15841 cctgaacgat ctcaaatttc caaggactta ataaaattct ggattcttcc aaaatttcta
    15901 ctagcatttt ttcaaaaacc aaactatcat ccaaaataaa tatacgcgtt accataaaaa
    15961 gtacctccac tcgatatttt actatcaaaa tcatttcata caacttttca acatacatgt
    16021 taggaataaa ttgtaattct cttcacaatc ttttccaaca cctttgaaac cgatatcaaa
    16081 tactttcagg atgctgcttc taaaataaat atcaaacaaa tgaatacatc atgcaaaaaa
    16141 agccagacaa ccgacgtcct tgcgtgtcag ctgtctgagc tatgtttatt tattttgttc
    16201 ttttaaaaag aacgatacac atactgattt ttcggagcag agactttctc agcgtttgta
    16261 acaactacag atgggatttc acgcttgaat ggttgataat agtctgattg gatggttcct
    16321 tctattttat accattcatc atttttaacc gttactcctt ccggcatatg cgtcaacaaa
    16381 ccaaagactc ctgaatctgc cacacaatgg ataatgccga atcggaacaa gaagatatct
    16441 gtattgctat cgttcggtgt ttggtaaaca aatccttcat agctgatttt tttgccaatg
    16501 aattcgctag ggtaattata aattagctcc ataacttcta aatagttttc atctgtgatc
    16561 ttgatagttt gcttatcccc atacttttct ttcaactcat tcatttggtc taagtagtct
    16621 gatttgttga aataaatact ggtatccggc tttaagtatt gtgtctgcat ctccggatcc
    16681 cctactgatt ctttacttac tgggaaatta aatcctttgg cttctacgat cgtagtgtcg
    16741 agactaactg ttggaaacaa cgtccccacg attactggca aagctagcag tacataagcg
    16801 attccacgtt gataaggttt tgacaaccca tggtcatgat cgtgctcgtg tgccttttct
    16861 tctgcttttc cgtttttgac ccacaaatac agctgaacta aagccaaaag agcagataaa
    16921 atcattgaaa gaatagctaa gtagcgataa tggacattga tatattgatc gagtttgcca
    16981 gaaacttgca agtacatcat caattcgaaa tacccagata aaatcaaaaa ccgcagcata
    17041 ttttccctcc taaataaata atgtgaatcc aataacgcta agggcaatca gtccgatcag
    17101 cgataaaata aagcgtcctg taaaatatcg tttcatcatc agtagatttt tgatatcgac
    17161 cattgggcca aagaccaaaa aggcagctac aggtgctgtg ccgaacagac ttaacaaaga
    17221 agatcctata aacgcatctg cttctgagca aagagacatc gtaaaggcca ataacagcat
    17281 gatgataatg gctaaaatct ttgttgagcc gagcgttagc ataattctag ttggtaaata
    17341 agtctgcatc gcagctgcga ttaatccgcc aatgatcaaa taacgacctg tatcaaaaaa
    17401 ttcgtcgatt gaatgtgtca acacatgccc agtttgatgg atcagctgat tttttggttc
    17461 attcttctca ctttgttctt cttcatggac atgatgatgg tgattttctg ctactttaga
    17521 cgtgattttt tctttcaata cagcgtcttt ttgaaaatag gcaagccaga caccaatcac
    17581 aagggcaaca aaaaagctgc ctatcacccg ccagcttgcc atctgccaag aattgccaaa
    17641 tgcgataaaa gtggaaaaaa tcacgattgg attgatgatc ggagcagtca gcataaaagc
    17701 aaacgctgtg tgaactggta cgcctttttt aacaaattga tgaacgatcg gtacgatgcc
    17761 gcattcacat gaaggaaaga aaaatcctaa gatcgaacca gtaaaaatag agaagatacg
    17821 attttcagga agccatctct tcacacgttc tggggtcaaa aatacttgta aggcgcctga
    17881 gataagacag cctaataata cgaaaggtaa tgcttcaata acgatcgata agaaaatcgt
    17941 actcatctgc aaaaccgaat ttggaataaa agaaaacatg agtttcctcc tttttatttc
    18001 tagttcattc gctttgcaaa tatacgcttt tattcaaaaa aaacaagttg ctctgtaaat
    18061 ttttacacaa ctcttatttt ttaccaaagc acttcaattg actttttctg ttattcccat
    18121 taaattaagg tctcatacat atcacttcta tcataaaagg gagaataaaa atgaaacaaa
    18181 ccactaactt gactgaagtt caagacatac tgcaacagtc atccgttgcc gtcatttatc
    18241 tttcgatgcc taattgcagt gtttgtcacg ctgttcgtcc acgactagag gaacttctca
    18301 cacattatga tattccggca cttcatttgg atgcatttga aacaccagaa gtagctagta
    18361 aatttgaagt gctgactgcg ccagttgtcc tgatttttca tcaaggaaaa gaagtctttc
    18421 ggcaagcacg gttcatcgac tttaaaaaaa tccgttatct actagatgaa ctgacagcag
    18481 aagatgattc cctttcttat gaagaaatct tccgaacaaa ataatcgttg tccaaacaga
    18541 aaaaagcgat tgaacaagtg aaagttttgc cttcatttgt tcaatcgctt tttattttta
    18601 ttctgctgta ttttcggaat gcgcatttgc gtcagaatgc aagaaataga tcaaggtttg
    18661 caattcattc gtcaaatcaa cattttggat caatacatct gacggagcat tcaatcgagc
    18721 agcggtaaag ttcaagattc ctttgattcc tgcttccgtt aactgattga ctacttcttg
    18781 tgctctttca gcgggtatcg ttaaaattgc tacttcgatt tgttggatct tgatttgttc
    18841 tgacatatct tccattggat aaacggggat accgtctacg atgcgcccaa cgatttcctc
    18901 attcacatca aaagcacaac tgatacgtat gctattactt tgatggaatt tgtattttag
    18961 taaagcactt cccaagttcc ctacaccgat cagcgcaaca tttgtcaatt gatcgtcgtt
    19021 taatgtcttt gcaaaaaagt gcataagatc ttctacatca taaccatacc cacgttttcc
    19081 taattcgcca aaataagaaa aatctcgacg gatcgttgca ctatctactt gaacggcttc
    19141 acttaattca gttgaagaca cttttttctt tccagcatta tttaaaatcc ttaaatagcg
    19201 ataatacaaa ggcagtcttt tagctgtcgc ctttggaata atatgatctt tcacgaaatg
    19261 acctccacgt attgtctaat tacatctttg atttctccat catagcagta tttataggaa
    19321 aaaatcaatt ccattgctca gatattgtga aattttattc tttattcatt tatttatcat
    19381 ttctgcaaat ctcgcagtat aatggaattt atggtagact actaattgaa aaatagtttt
    19441 ttatttgagg agaattcaca aagtagaaaa gaggattcca tgatattatt acaagccaat
    19501 cagattgctc gccattttgg tgcagaagtc ttatttgaaa acatacattt agaaatcagt
    19561 acaggcgctc gtgtcgcttt agttggcaga aatggcgctg gaaaatcgac tttattaaag
    19621 atcattgctg gaattgaagc acctgatgaa ggaaagatcg ctaaaaataa aacagcgact
    19681 ctcggatacc tcgctcagga cacaggactt gattccaatg aaacagtttg gaacgagatg
    19741 ctgaaagctt ttgaacaagt tagacaaatg gaagagcgga tgcgcgagct agagattttc
    19801 attagcgaag gtacgccaga cacacctgct tatagttctt atttaaaaga atacgaccgg
    19861 ctgcaacacg atttttcaga tcgaaacggc tatggttatg aaaatgaaat tcgttccgta
    19921 ttgcatgggt ttaaattcga ttcttcattt tatgatcaga aaatcgatac tttatcaggc
    19981 ggacagcgca cacgtttagc tttagctcgc atgcttttgc aacaccctga cattttgatt
    20041 ctagacgaac caacgaacca tttagatatc gagacacttt cctggcttga gaattatctg
    20101 caaagctata ctggtgcatt attgattgtt tcccatgacc gttatttttt agataaagta
    20161 gtcaatgaag tctatgaatt aagcagaaga aaaatgcgcc attacaaagg taactattcg
    20221 aaatatctag atttgaaagc agaacagtta gcaagtgaat ggaaggctta tgaaaagcag
    20281 caaacagaaa tccataaatt agaagacttc gttgctcgga accttgttcg cgcctctacc
    20341 acaaaacgag cgcaaagccg cagaaagaca ttagaaaaaa tggaccggct ggatcgtcct
    20401 caaggggatg aaaaacaagc aaactttcta ttcagtatag aaaagccttc tggaaatgta
    20461 gtcttgcaaa tcgaagatgc cgctatcgga tatgataatg tcaccttatc tgaaccaatc
    20521 aatctagata tccgtcgtca agaagcgatt gctctagtag gtccgaatgg tatcgggaaa
    20581 tcgacgttgc tgaaatcaat cattcaccag atccctttta tccgtggaaa agaaacgcta
    20641 gggacaaatg tcaccatcgg ctattacgat caaggtcaag ctgatctgca tacaaacaag
    20701 acgatcctag cagaattgtg ggatgagcat cctaccactc cagaaaaaga gatccgaagt
    20761 gctttaggtg ctttcttatt ttctggcgaa gatgtagaaa aaccaatccc tctgctaagt
    20821 ggcggtgaaa aagcacgggt tgctttagcc aagttagcga tgaacaaaga aaattttttg
    20881 attttggatg aaccaacaaa ccatttagat attgacaaca aagaagtatt agaaaatgca
    20941 ttgatcgatt atgacggcac actgcttttt gtttcccatg accgatattt catcaatcgg
    21001 atcgcgacaa aagtcgtcga actctctgaa aatggcagca aagtctatct aggagactat
    21061 gattattatt tagagaaaaa gcaggaagaa gaagaaatcg ccgaattgtt ggcacaagat
    21121 gagccagaat ctgctcctgc tcccacgaac aaatctgatt attaccaaag caaagaacag
    21181 caaaagctta tccgtagcct tcaacgaaaa attactcagg ttgaagaaga aatggcgcgg
    21241 atcgatgaat ggatcgatca gttggaacaa gaaatggtcg cgccagaaaa cctggaagat
    21301 cacgtaaaac tcaatgagct gaaccaagat ttagaagctg ctagacaaga tcaagaaaca
    21361 aaattgacgg aatgggaaga actaagcttg gaattagaag aatatgaaaa ttaacgaaaa
    21421 aaccaaaaca gaaaccattc atgctgtttt ggtttttctt tttttatttt atcaaacaaa
    21481 aattccatat ttaccttttt caccaataag aagaccaatt actagtcatt ctcactaaag
    21541 cttctaaata atagtaatca ccccaaatac aaggtgtatt taccccatta tttttgggct
    21601 tattataaac agactgtgtt aaaaagccat cttcttcttc cgttgtagca tatctttctt
    21661 tcaattgtcg cataattttc caaacagcag actggtataa gttcttttct gttttgtcta
    21721 atagacctga ttctattaat tctaataatc cacaaaccat gatcaccata ctagatgtat
    21781 ctttttcttc tctgctagaa tcatcaaaga tcaaatccca ataacaaatg gtatcttctg
    21841 gcaaccgatt cataaaataa ttggctaata ttttgcttaa atcaaaatat ttttgttctt
    21901 tcaaataacg ataacttaat gcaaaaccgt aaatccccca tgcttggcct ctagcccaac
    21961 aggaattatc actataacct tgatgcgtac ttccatatct tggttttcca tctttgacat
    22021 ccatataaaa tgtatgataa gttgaaccat cttctcttac taaataatct gctgcatttt
    22081 gaatatgagc ctgagcagct tttttatatt tttgatttcc agtttcttca ctcgcccaat
    22141 acaataatgg aagattcatg caacaatcca tgatcattct accttgttgt gaaggatcac
    22201 taggatctcc ccaagcttgt ataactcctg ccttctccca aaaacgctga agtaatgttt
    22261 ctgcagcatc aattgaagct tccttcattt ttcgatttcc cgttatttta taatctgcta
    22321 cggttgataa agaatacaaa aaaccaagat cgtgatgatt agtgataatt tgacagtcaa
    22381 tcctgtggat aaaatctata tctgcttttt cagctgtttg ttgaaataaa ggatcacccg
    22441 tcttctcaaa taacaaccaa ttaattcctg tccaaaatcc ttctgtccaa ccaatattgt
    22501 ctcttaatgg ataacgtcct tttataggac actcgcttgg gaattgatac tgactgttac
    22561 tgatacgttt gattaacctc tccgtgtgtc tgcgacaacg ctcgatctca ttattccatt
    22621 ctttttccat taattcttta taacattcat ttatccattt ttctggatgt attatctctt
    22681 caaacatcac tttatcaagc atttccccac cagcctttag cataatttta gtcttttttt
    22741 attgttttta ttttggatag ttagcccggt tgtcccatca aaatcaattt tgggacgatt
    22801 gttccatact tctaaactat aatcccgatc ttgatgagca accactcctg ttccgagcca
    22861 atgggttcct tttccaaatg taccctctaa tccaggaata aaagtgtttt cccatgtcat
    22921 caagtttgta ttgggcaaac ccataatagc tgtggatttt cgtttaatag tatcgtcact
    22981 taaacagata atacctgaaa agccagcttc actcacccta tagttttcct tgtcttcttc
    23041 tatgattatt ccttctttct caggagaaca ttcaatagca aaccctgtct catatagaac
    23101 atattctgta tctaattcta ctttgtgtac gcgaatctgc cagccttcaa aaggaataag
    23161 ccaagttctg atttttgcct gtaaagtagg ttcccatgta gaggcaagta cctctttctt
    23221 acatacaatg tctcgtacat tatttcgaac gatgaactgt ccataggttt tgatgctctc
    23281 attcttaaaa ctggtcgtga tatatgtgtc cgctcgctga atccctagca ttgaatccat
    23341 tgccatttct gctttcaatt gattcgctcg cggtatcgaa aaaccaaatt gactagaata
    23401 agcaaatttt aaatattttt cattggtcaa tgtatggaaa tttggcccta attgtcccgc
    23461 attcaaaaaa aatgtatgtc cattatcatg ttgtatgagc aaattggctt ctgaaagaag
    23521 tttagttcct tcacgaatag gcataggcat ttctttagcc gtccaatact catggtcttc
    23581 aggtagcgcc aacaataaaa aaatcttatt cagccataat ggagataacg tactattata
    23641 gggttcagtc aatgtcaatt gagggtaggt ataaccaata ttcaggatac ctttttcatc
    23701 aaatatttct ttttgcatcc accatcttat attccgattg actaacccct tgatttcacc
    23761 taatttaaaa ggcaaaagat cggcccacaa catagctgac caaaagcttg aaactgcaaa
    23821 acgataaatc atgcttcgac caaaaggcac atttgcacca tcttccgcaa aaaaatgaat
    23881 atagtctcta gaaaaatcaa tagctcggtt cttatatatc tggctaatgg gcttttctgg
    23941 ttctagtacg ctataaatca aaccataata atgaaatgca aaaggattat aataatcttg
    24001 gcgacctctt gaactgtctc gataccagcc attttgaaga taacaatttt ctatattatc
    24061 aaatgcttct ttcagtgctg attctttaac tggataacca aatttttgga taattgttcc
    24121 gacaattatc ttaaaaaatt gccaattacc atctgcacat tcatattcaa aaatttgcga
    24181 gaaccaatga ataatctggc ttttttctcc tgtatctaat tttttccata ataattgttc
    24241 atgatgatat aacgctaata acattgcggg catttctacc atacgctggt ctctttcatg
    24301 tatttctccc caataatcgg gatgggaagg atctgttcca gcttttattc ctgatagaat
    24361 taatttcaac catcgttcat ctaattggtc aattgcgggc cccgctcccc ataaaacccg
    24421 agagaatcct tccattcctg caattttatt tccatatcct accccttgat gtatataatc
    24481 aatttgtgcg ccacttgctc gataaaacgg ctggagtggt tgatttatct ttttaaataa
    24541 gtcaaagtaa tcctgtctgg tccttaatag tggtactgga tgtttcattg tattttctcc
    24601 tctttttact accaaatagc taattccgac tgttctaatc gaagtaagcc ttcaataaaa
    24661 aaataatcag aatagatcag tggcacatta taagtcagtt ctctatggta tgcttctgct
    24721 ccgcctccaa tgatcccatc ttcatccata ttccaatttg catagtgctt ttctgtcttt
    24781 tggagtatat tgatagcagc ttcatgatac atttttgatc gtacagagga aacatatcct
    24841 gctaattcca gaagaccgca tgcagcgcat actccagcac tggtatcaat attcgaatct
    24901 tttacagcaa aatcaatgag aggaatataa ccagtttttt cgattttctg aataaaattt
    24961 actgctatct tttctgctat agctagatag tacggatttt ttgtgtaacg ataacttaac
    25021 gcgaatccat ataaagccca tgcatttcct ctgctccaac aagaattctc attgtatcct
    25081 tgcccgccaa gtaaccgaat atatttccct gattctgcat caaattctgc gatatgcccg
    25141 gttgatccat ctttcctcac tagatactta gctgttgtat ctgcatgctg ccgagcaatt
    25201 ttagagaacc tcggatcacc agtttccctc gaagcccaat acagtaatgg taaattcatc
    25261 atactatcaa taatcatcca accaggttta tggtcattcc atgctactaa atagttccct
    25321 gaaatattat agcgtccagc aagtaccgta gctgcatgta accctagttg tcgactatgt
    25381 ttgttttgag actgttgata atgtgcaata gccgatggta agaataaaaa acctacatca
    25441 tgatgaacgt ctgaaaaatg gtacagggca gattctaata attgttcttg ttgtattgct
    25501 gcctctttga acttttctcg ttttgtcttg tgaaagagct gccataaaat accactccaa
    25561 aaaccattgg tccaccagtc gattcctctg tcctttatta cgtctgtata ttttccgttg
    25621 taagaaatat atggaatttt attccccatt ctgtctaatt ccgcatctat cttttcagaa
    25681 gctacattca aaatttttct tgaccattcc ttcgtctgtt cattcatgga tatcccgcca
    25741 ttcttttaat ttttatgaat gtggagttgc tcctagaacg aactagatgg ttaacattcg
    25801 ttattttttt tacttatttg cagctccaat gtcattctcc ttaaatttag actgcgccca
    25861 cacataaatc aagaccattg ggatcactga agttgctaaa attagcataa ttgcgccata
    25921 cttaattggc tcacctgtac tagaaaccgt catatttttg ataattaagg gaaccgtata
    25981 tttatcttta tctgaaagca tgactaatgg aaatagatat tgattccaac ttcccataaa
    26041 gataagtaaa ccactagtaa taatcgtagg tgataaaatc ggtacagcga ttttcgtaaa
    26101 aatcacccac tcattggcac catcgattct ggcagcctcc atcagtgctg taggaaattg
    26161 actgctatat tgtcgcatca taaacactga agttgtactg gcaatcgaag ggatgatcac
    26221 acctgcataa gtattcgata atcctaaacg actcataatg taaaataaag gaatcaatgt
    26281 ggtaaaaaaa ggcaccatcc tagatagcat aactgtattg aacaacagtt tattccccat
    26341 aaaacgatac tttgctaatg cataacccgc cattgtagaa ctggctgtag ctgaaactgt
    26401 actgattaca gcaataacaa tcgaattcca aataatcaag ggataattat atctcgccat
    26461 gatggcttgc cagtttttta atagcatatc cccaaacttg aaattgattc cgccacttaa
    26521 aatttcttga ttactttgcg ttgaagctac aatcatatat aaatatggca tcaaaaatat
    26581 taaggcacca aacacaataa tgacgtacgt aaaaattctg ctttgtatct tttgtttagt
    26641 catttttatc acctccgaat ttaaattgaa tagccgataa tataacagat acaaacacca
    26701 tcgtccaggc aatagcagag gcttttccaa aatcaaccat ttcaaaactt gttttatata
    26761 aattgactcc tacgaccaaa gctgaatttt ctggtccatt ggaagtccca aagaatatat
    26821 ttgggatatt gaaattaccc aagccattga tagtggctaa aactgtggcc attaaaatga
    26881 ttggctttag catggggatc gttaataaaa agaattgccg aaatttcgat gctccatcta
    26941 tttcagcact ttcgtatatc tctttagaaa tcccttgaag accagctaaa aataaaatag
    27001 tgttatatcc tacactaagc caaatcatcg tgatgatcat tgatattcgt atccaaaaag
    27061 gatttgttaa ccactgtaca ccgttaattc ctagcgcatt caaaaatcca ttgaataacc
    27121 cagtcggatt gaaaaaagtc atgaatacgg aagctacagc caccgttgat gtaatggttg
    27181 gaatataata gacagttcta aaaaatcctt ttccttttaa ctgtgctgaa ttcaataaaa
    27241 cagcaaagta aatagctaat agcatcgtta ttggaattga tcctgccatg ataatcaaaa
    27301 cattcgtgta agatttaata aacaatggat ctcggaagat agctagataa ttgtcaaatc
    27361 caacaaattc aaatgagtag ccaattttca taaaactaaa agcaaacgag ataatgatgg
    27421 gaatcaaaat cgtcacaata aagaaaataa caaaaggtgt gaccaatcca taagcacttt
    27481 ttttatgaga cgtatctgtt gcttttttct tgctgttctt tattgtcttg cttggtaacg
    27541 aagattgcat catcgttaag tcctcctaat attttttaat caccagttaa ttttacgccg
    27601 tacttatgag caaaattttc tgcttctttt agatatgaat tttcaaaatc acctgtttgc
    27661 caatagttat atgcagcggt agtcaaataa gacgtagcat ctgaataagt ggctggtaaa
    27721 acagtatttg atgtattttc attgatttta ttcaatagtt catagtattt ttgtcctcct
    27781 tagtagtcaa actttttctc agctgcttct gtttgatagg ccaatgagtt agatgtagcc
    27841 acgcccagct ccagtgcgtt ggctaagcat tcttcatctg tcagcataaa tgtaagaaat
    27901 tgttgtgcca cttgtggatt tttggcatct ttagggacat aaaaacttga tcctcctgat
    27961 actgcacctt ttcctggatt ttcttttgaa aaaggaatca gtggagccat gcgccatttg
    28021 tcagatgcat caggaaaatt caaagacata ttcgtagcat accagccgcc agcaacaaac
    28081 attgcagatt cttgaaatgc tttctcagca tcttgtgcac cgtagaaatg tacaatatct
    28141 gagtcgatca tttctttgat taactttgca gcatgtttcg cttctttagt attcagacgg
    28201 atatttcctt cttcatcaag caaagattga ttctgctggg ctagcatgtt gataaacaat
    28261 tcttggtccc cagattctgg caaggcaatt ccaaaactac cagttttttc ttttaatgct
    28321 tttgccacct ggatatattc ttcccaagaa ctgatttctt caaaatctac acctatttca
    28381 gcaaattttt ttggttgata ataacaaagt gctggggtaa aatcactagc ccaaggaata
    28441 atcgtttcat tactcgtttg ttgacgcaaa atattcaatt tttgatcata aaactgatcg
    28501 ccatatttgt cataaaatcc atattcatca gcactataaa aagcatcttg aaatttttcc
    28561 agcacacctg ctgctgaagt gtctgccatg gagataatat ccggcatatc ttcgtgtgtt
    28621 accaattttg gggtgatcat ttgtactgtt gctgttcccg aagctacatt cacaattttt
    28681 aagtcaatat ttgctccagt tttgcggtta tagatttttg cagcattttc aacatagttt
    28741 gtcagtttgc cgtttgcgac atatacttct aatgtagtgg ctttttttat cattggatct
    28801 gcgatgtttg ctgttacttc ttcgttagtt tttccacatg cacttaagct gaataatagg
    28861 gtaatgttta gcaaagctat caccaatttg tatctttttt tcatgtctat cccttctttc
    28921 ttttcaagtt cttatataaa acgctttcac ttatgtatat taacggaatt agtaacacaa
    28981 atatttatat aaaaaaatat tttacttgtc gttttggctt ttcatttttg ttataatagt
    29041 cattaattat aaatagaaag ctttttatat aaaaagagaa aggaaagtgg ttcttttgtt
    29101 aacaaacaac caatcctcca atcttgtcaa aaaaacacta acctattcca tagataccca
    29161 tcttcattta tctgctgctc cctattttcg actactaata aatgaaagcg ctaatttata
    29221 tgctgaattt gcacataaat ctatagaact agataatcct tcccttttat tcattgataa
    29281 acagcaagaa gtccaaatct tttctactca aaaggaaaca caaactttta tcattatcca
    29341 gttttatttt gatgagcaat tattagcaca tattacggga caatcttttt ctatagatca
    29401 agaaataaca tctatcatta cttttctgaa aaatgaacag ttcaaaattg attatctaaa
    29461 acatagtaag ccagctattt cctatgatca ggaaatcaat tcttttttca tactatgcca
    29521 acagatgagc attcatttgc tttccttatt agttacttat tgtctgcaaa aaattcaatt
    29581 gtcttcctta ccaaggcatt atcaacagga acttgcttta ttgacaatac cttcaaatga
    29641 gaaagtcatt cctaaaaaaa agcatattct ttattcagat gaattagttg atccaatcat
    29701 acgatattta gaagaacatc tttcagaaaa ttttttgctt gaacaattag cccaaatttt
    29761 ttttatcagt ccagccagct taaaaaaaca ttttagaaaa gtgaatggaa aaagtgttat
    29821 tagctatttt aaagaactaa aaatggaaaa agcaaaacgt ctcgtgctag aaaatgaatt
    29881 aacctataca gaaattgcag atatattagg atttcattcg atccatcatt tttcttcagc
    29941 ttttaaaaaa tatacagggt tatccccttc aaagtattat ctatccttaa aatagaaaaa
    30001 aatttttagg ttttatccac aaattatttc tctatatttt ctgtctattc aaaataaaca
    30061 ataattttcc ggcacaatta tttgctgttt tttaccataa catagtacga tcaacgtaga
    30121 aaaggagatg tttttatgaa agttgttgtc attggcggtg tagctggcgg tccttcattt
    30181 gccactcgtt tccgtcgatt gaatgaagcg cacgaaatca ttatctatga acgcggagaa
    30241 aatatttctt atgcaagttg tgctttgcct tattatttag gaggtgtgat cactgaccgt
    30301 gactcgctga tcgaacgtac accagaaata ttgaaaacaa aaaacaacat cgatgtcttt
    30361 actaaacacg aagtaacagc aatcgatcct tctactaagc gattaacagt taaagaccta
    30421 tccacaaatg aagaaataaa aacagattac gataagttga tcatctcttc tggtgctaga
    30481 ccagattatc cggatattcc cggagttttt gaagcagaaa acggctttgt cctccgtagt
    30541 gtgacggatg cggatcgaat caaatcgttc cttgaagaaa aaaatccaca acatgtcgtc
    30601 attcttggtg caggtgttat gggtctggaa ttagctgaga atttcaagca tcgcggctta
    30661 gacgtgactt taatcgatca attgccacaa gtcgctttcc cttatgatcc agaaattgct
    30721 aatttagttt atgacaaatt gctgaaagaa ggattagccg ttcatttaga aacaagagtt
    30781 actgagatcc gtgataaagg tcgagaaata gtattatcag atggctccgt cctttctgct
    30841 gatatgctaa tttttgctgt tggtgtttct ccgaagaatg aagtggtgaa agcaaccggc
    30901 atacaattat cagatacagg tcagatcatt gttgatgacc agttacaaac caatctgccg
    30961 gacatctatg cgattggcga tattatcgaa acgactagtg tagtgactgg tcagccgatc
    31021 caaagtatgc tttccagtgc ggccaatcgt caaggacata tgctggcaga tattttaaat
    31081 ggtacgccta tgcgctatcg cggatatatt ggtgcaggtg tcgcaaaaat ctttgatcat
    31141 acagcaagtt atgcgggaat gacagaacat gaattaaaag catcaggcat aacaaattat
    31201 aaaactgttt ttatcactcc ctttgaccat gcctatttct atccagaagc tacaagatta
    31261 aatctaaagc tgatttttga tgcagatagc ggtcgtattt taggtggaca agcatttgga
    31321 gaaaaaggtg tcgataaacg gatgggagaa ctttctgtag caatcaccgg aaacttgaca
    31381 gtctttgatt tgcctgattt ggagttgcct tactctccac cgtattctac tacccgtgac
    31441 ccgttgaata tagctggtta tgtcgcaatc aatcaaatga cgaatatcgt agaaacgatc
    31501 aaagcaagtg atatacccga aaacgatttg aaagaagcgt tctttttaga tatacgtgaa
    31561 cctaataaag caccttctgg cagtatctcg gcaacaaaaa atattccaat gaatgagtta
    31621 cgagatcgaa tcaatgaaat cccaaaagat aaaaaagttt acattacttt cagaagagga
    31681 ttgaatactt atacttctgc ccgaatcttg gcaggtttgg gtatcaaagc gattttgatt
    31741 gaagaataaa aaaacgaccg cggtgggtag ccgcggtcta ggagttattt ttacttgagg
    31801 agtaaaaatg aaaaagtata tagggttgtt ggttggtatg tatatagaat agaagataaa
    31861 tgtgaagaaa ttgtgcatta tttcttcaca tttattaggt tatagtttaa gaaagtttaa
    31921 acattgcgca atttaattat tttagctgtt tcttttcata aaaaaacatg ttgtgactct
    31981 gagacaaatc atagtccata gtgatgtctc tagagtaact acgcctaata ttacgaggca
    32041 tcatattaat ctatcagcac tagtacctaa actagcatcc ctttcgaata ttttttctta
    32101 tataataaag tttgctttca ctcctctaat ctcaccaaat cgactggctt gcgttgtgtg
    32161 atactaggaa ctgattggtt tagaacgact tttctattgt aatcatattt ttcgccatcc
    32221 tcgttgtctt ctaatccttc aatgaatgat ccctaatgtg tgtattaaat aatttaattt
    32281 ttttgttcct atttattt
//
LOCUS       DACGLE010000026        30547 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0534
            SAMEA3304449-rid9560453.denovo.027, whole genome shotgun sequence.
ACCESSION   DACGLE010000026 DACGLE010000000
VERSION     DACGLE010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304449
            Sequence Read Archive: ERR985064
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 30547)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30547)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702225.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702225.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 20:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,045
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 27 of 67
            Pseudo Genes (incomplete)         :: 25 of 67
            Pseudo Genes (internal stop)      :: 19 of 67
            Pseudo Genes (multiple problems)  :: 4 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30547
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304449-rid9560453.denovo.027"
                     /strain="VREN0534"
                     /db_xref="taxon:1352"
     gene            492..1199
                     /locus_tag="IXT20_001318"
     CDS             492..1199
                     /locus_tag="IXT20_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ4859773.1"
                     /translation="MSNQLPVYIQIHDQLKNEIEQGIWKVGDRLPSERELAVKFNVSR
                     MTLRQAIQNLADEGILERKIGSGTYVAREKVQEKMSGATSFTEIMESQNRVPSSRTIS
                     YFLTSPSSSEMEKLNLSKDDTILRMERIRFADDVPICFEVASVPQKLIDGYSKAEITE
                     SLYRTLEEKGGKKIGPANQTITAVLASEKIADYLEVKRGDAVLRLRQISYFEDGTPFE
                     YVRTQYVGNRFEFYLEK"
     gene            complement(1258..1902)
                     /locus_tag="IXT20_001319"
     CDS             complement(1258..1902)
                     /locus_tag="IXT20_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HAQ4859774.1"
                     /translation="MIERYRTIKENGQSEIEIKKSRFICSLRRIESEDQAKSFIQEIK
                     KEHWKANHNCSAFIIGEKNEIQRSSDDGEPSGTAGVPMLEVLKKNQLINVAAVVTRYF
                     GGTKLGTGGLIRAYTHAVSQALSDIGIVEGTLQQEISVMITYPQLGKLQNYLELQHFF
                     IKEIQYTDQVSVICAVDEKSADHFKESIIELLNGQVQFEEGSVSYHEQLITDHI"
     gene            2029..2316
                     /locus_tag="IXT20_001320"
     CDS             2029..2316
                     /locus_tag="IXT20_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4859775.1"
                     /translation="MQERMKKYDAITHYLKNNGGSQVTLTFTQFDELLFPSNGLPKTA
                     RESTDWWANDYKHPEKGAYGWINAGYEVVVINLDKEYVVFNKLVKSSWLFD"
     gene            2424..2744
                     /locus_tag="IXT20_001321"
     CDS             2424..2744
                     /locus_tag="IXT20_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ4859776.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEAKGIDSDIFAVSASDA
                     DNNLANKDVDVLLLGPQVRFMKADFEKRLEPKGIPLDVINMADYGMMNGEKVLDQAIT
                     LIGE"
     gene            2825..4138
                     /locus_tag="IXT20_001322"
     CDS             2825..4138
                     /locus_tag="IXT20_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HAQ4859777.1"
                     /translation="MNHFWGRRLLTRDIEAIDCEEVNLPNYEKIAAVERLGNWILCHR
                     CGMRTSVIDGQLADYGYFCTHCLYLGRCDTQQDLYLFDQPEAKPREVVFFWTGQLTEK
                     QTEISKRILCHSEKRHHLIWAVTGAGKTEMLYPILVETLKAGGRVAICTPRVDVCNEL
                     FLRYRYVFPEEKITLLHGNTATAYTFTSFVICTIHQLYRFYRSFDLIVIDEIDAFPYN
                     GDAGLAHAVQTAIKPDGRFIYLSATPDKKLLKEIKQTFDIHKLALRFHRRLLPEPVLV
                     FMNNWRQKCKNKKKIKSLVRIIHQLIKENHILLFCPSITMMHRLKATLQEALPRYEIT
                     DVSSRDNQRAEKVQKMREAKYDILLTTMILERGVTFERISVIVLGADHPVFTKSSLVQ
                     IAGRADRKGPYTNSQVYFFYEEKTAAIRKACQEIKEMNEEGKKML"
     gene            4135..4854
                     /locus_tag="IXT20_001323"
     CDS             4135..4854
                     /locus_tag="IXT20_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HAQ4859778.1"
                     /translation="MRCSCCSKDIFQNLTFSEIIGVSKYRLPQTCSVCRAQFHLLETR
                     GCSGCQKPIHFNLESTKKKVKSIPVYCSDCQEWKKKYPTYDFQHRAFFVYDENMKAWL
                     NQYKFMGDIRLAGTFAYKWTQIKKEYPTYIYCPIPLSEKRRTERGFNQVSEMLKAARI
                     PFQMLLKREKHLLAQAKNTRKERLEMPQPFELAVTFSKIQNKKILIIDDVYTTGQTLF
                     HAADCLQVAQPKIIRTISLAR"
     gene            4982..5533
                     /gene="raiA"
                     /locus_tag="IXT20_001324"
     CDS             4982..5533
                     /gene="raiA"
                     /locus_tag="IXT20_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HAQ4859779.1"
                     /translation="MFRYNVRGENIEVTEAIRDYVEKKVGKLERYFSDAPDATAHVNL
                     KVYTEKTAKVEVTIPLPYLVLRAEETSPDLYASIDLVVDKLERQIRKFKTKINRKSRE
                     TGMNTADAAALFNEEDENENDSELDIVRTKRLSLKPMDSEEAVLQMNMLGHNFFIFED
                     AETNGTSIVYRRKDGKYGLIETD"
     gene            5755..8289
                     /gene="secA"
                     /locus_tag="IXT20_001325"
     CDS             5755..8289
                     /gene="secA"
                     /locus_tag="IXT20_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HAQ4859780.1"
                     /translation="MANLIRSLIENDKKELKRLDSIAKKVEAYADQMAALTDEQLQAK
                     TAEFKGRYQKGETLDQLLPEAFAVVREAAKRVLGLYPYHVQLMGGIVLHDGNIPEMRT
                     GEGKTLTATMPVYLNALTGEGVHVVTVNEYLATRDSTEMGELYNFLGLTVGLNINSKS
                     SDEKREAYNCDITYSTNNELGFDYLRDNMVVYRHQMVQRPLNYAIVDEVDSILIDEAR
                     TPLIISGQAEKSTALYTRADNFVKRLKEEEDYKIDVQSKTISLTEAGIEKAEETFGLD
                     NLYDIENTALTHHLDQALRANYIMLLDIDYVVQDGKVLIVDQFTGRIMDGRRYSDGLH
                     QAIEAKEGVEIEDETKTMATITFQNYFRMYKKLAGMTGTAKTEEEEFREIYNIQVYQI
                     PTNRPVIRDDRPDLLYPTLQSKFHAVVQDIKDRYRKGQPVLVGTVAVETSELLSDMLN
                     QERIPHEVLNAKNHFKEAEIIMNAGQKGAVTIATNMAGRGTDIKLGLGVRELGGLAVI
                     GTERHESRRIDNQLRGRAGRQGDPGMSQFYLSLEDDLMKRFGSERIKAFLDRMKIGEE
                     DAVIQSKMLTKQVESAQKRVEGNNYDTRKNVLQYDDVMREQREVIYKQRQEVIMEEKS
                     LSKPLMNMVKRTISRVVDAHTQLEKENWNLESIIDFAGSTLVHEDSITLADIEGKTSQ
                     EIKEYLNERAQEVFNTKAAQLNGPEQLLEFEKVVILRVVDSKWTDHIDAMDQLRQSIG
                     LRAYGQNNPLVEYQTEGYKMFEDMVGAIEYEVTRIFMKAEIRQNVQREQVSQGTASHS
                     EDGDAKENSNTSAKKQPIHVNKVGRNDPCPCGSGKKYKNCHGKNAN"
     gene            8415..9516
                     /gene="prfB"
                     /locus_tag="IXT20_001326"
     CDS             join(8415..8477,8479..9516)
                     /gene="prfB"
                     /locus_tag="IXT20_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_078989099.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HAQ4859781.1"
                     /translation="MEISEIRNLLTEMQEKVTSFRGLFDLDQLEEDIAEAEAKMAEPG
                     FWDDSEAAQKVISENNANKETYDQFHSLAEELEEIELMVEMLQEEPDAAMQEEAETRI
                     NALDEKMKTYELSMLLDGPYDRNNAIVELHPGAGGTESQDWGSMLLRMYTRWAEQHGY
                     QVETLDYQAGDEAGIKSVTLLIKGHNAFGYLKSEKGVHRLVRISPFDSAKRRHTSFCS
                     VDVMPELDETIDIEINPDDLKIDTYRASGAGGQHINKTESAVRITHLPTGTVVASQAQ
                     RSQLKNREQAMGMLKAKLYQLEVEKKEQEAAALRGEQKEIGWGSQIRSYVFHPYSMVK
                     DHRTNVETGNVQAVMDGDLDLFIDGYLKMHLQ"
     gene            9621..10307
                     /gene="ftsE"
                     /locus_tag="IXT20_001327"
     CDS             9621..10307
                     /gene="ftsE"
                     /locus_tag="IXT20_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005237293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HAQ4859782.1"
                     /translation="MIEMKDVMKKYSNGTTAIRNLSIVIEQGEFVYVVGPSGAGKSTF
                     IKLMYREEKASKGTLNVAGHDLIAIKNREVPYLRREIGVVFQDYKLLPRKTVYENVAY
                     AMQVIGRKPRDIKKRVMEVLDLVGLKHKVRVFPSELSGGEQQRVSIARAIVNTPKVLI
                     ADEPTGNLDPENSWEIMKLLDRINAQGTTVVMATHNSTIVNTIRHRVIAIENGRIIRD
                     QAEGEYGYDD"
     gene            10291..11184
                     /locus_tag="IXT20_001328"
     CDS             10291..11184
                     /locus_tag="IXT20_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ4859783.1"
                     /translation="MDTMIRTFFRHLLESIKSLKRNGWMTVASVSAVTITLTLVGVFM
                     AVIMNATKLAQDIEGNVDVSVFVDIGTKDEEMKKLEKELNDLDHVKKVEFSSKEQELK
                     KIQNEMGDSWKLFEGDNNPLYDVYVVSATDPSYTKQVAEEAADLKNVSRSDYGGASSD
                     RIFQIAGAVRTWGLVAAALLLFVAMFLISNTIRITILSRQREIQIMRLVGAKNGYIRW
                     PFFLEGGWIGLIGAILPILIITFGYAWFFQLINPSLLRSHYSLIRPQNIVWKINLLMV
                     GIGVIIGSLGSIISMRRFLKF"
     gene            11336..12784
                     /gene="cls"
                     /locus_tag="IXT20_001329"
     CDS             11336..12784
                     /gene="cls"
                     /locus_tag="IXT20_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HAQ4859784.1"
                     /translation="MKIIVDNFFTILLIMNILLSFIIVFRERKETAQTWAWLLVLMFI
                     PVVGFILYIFLGRGISKEKIFDLKIQGKIGKNLEIEEDKQALMRGLYPHPPTGQVDVK
                     QLIYMLTVFESTLYTTKNEITLFTDGREKFDALIEDIKQAEDHIHFQYYIYRSDALGE
                     EVRDALTEAARRGVKVRVLLDAWGSTQVSSSFFQNLKKAGGEVAFFFPLFVPYINPRI
                     NYRNHRKIVVIDGKIGYTGGFNVGNEYLGLVKKFGYWRDNHLRIYGEAVYTLQNRFLM
                     DWNSQHTKEVGYSPKFFPSIHTTGDIAVQIVTSGPDTEHEQIKMTYLKMISLAKREIL
                     IQTPYYIPDGSIHEALKLALLSGVKVHIQIPNKPDHLLVYWATYSFAAELIEYGARIE
                     TYENGFIHAKTMIIDGGIVSVGSANIDVRSFRLDFEVNTLVYDERMASRVRKAFFEDS
                     KISTHLTKEMYENRGIIIKMKEGLARLISPLL"
     gene            13121..13978
                     /locus_tag="IXT20_001330"
     CDS             13121..13978
                     /locus_tag="IXT20_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HAQ4859785.1"
                     /translation="MKKLKLFLPIIGLAILLSGCSKWIDRGESITAVGSSALQPLVET
                     VAETYQTENPGKFINVQGGGSGTGLSQVQSGAVDIGNSDLFAEEKSGIDASALVDHRV
                     AVVGITPIVNKDIGVKDISMENLKKLFTGEIKNWSEVGGKDQPVVILNRASGSGTRST
                     FEKWVLDGETAVQAQEQDSSGMVRQIVADTPGAISYVAFSYVSDDVDTLNVNGVEPTD
                     ENVTTNKWTIWAYEHMYTRDHPKKLTKEFLDYILSDEVQDNIVGELGYIPVSHMKVER
                     DWQGNIVKE"
     gene            14093..15013
                     /gene="pstC"
                     /locus_tag="IXT20_001331"
     CDS             14093..15013
                     /gene="pstC"
                     /locus_tag="IXT20_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HAQ4859786.1"
                     /translation="MEDVKKVLLTKSKRSKMEQRGKFISFLCIALIVLVVLSIFYFVA
                     SKGLATFFTDKINVFDFLFGTDWNPSQIGANGKPMVGALPMITGSFIVTFLSAIVATP
                     FAIGAAVFMTEISPKNGTKILQPVIELLVGIPSVVYGFIGLSVIVPFVRSIFGGTGFG
                     ILAGTFVLFVMILPTVTTMTVDALKAVPRHYREASLALGATRWQTIYKVVLRAAVPGI
                     LTAVVFGMARAFGEALAIQMVIGNAALMPTGLTTPASTLTSILTMGIGNTIMGTVENN
                     VLWSLALILLLMSLFFNIVIRMIGKKGELK"
     gene            15013..15897
                     /gene="pstA"
                     /locus_tag="IXT20_001332"
     CDS             15013..15897
                     /gene="pstA"
                     /locus_tag="IXT20_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010764435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HAQ4859787.1"
                     /translation="MNAKRSDKIATGVLYIVSGIIVLILAALLLYILVRGIPHISWSF
                     LTEPSKAYQEGGGIGIQLFNSIYLLLITMIISFPISLGAGIYLSEYAGKNWLTDVIRT
                     SIEILSSLPSVVVGLFGFLVFVIQFQYGFSIISGALALTVFNLPLLTRTVEESLQAVH
                     YTQREAGLALGLSRWETVMKVVVPEATPGILTGVILSSGRIFGEAAALIYTAGQSAPA
                     LDFTNWNPLSVSSPISIFRQAETLAVHIWKINTEGTMPDGVSVSAGASAVLIIAVLLF
                     NFGARAIGKRLYRKMTSA"
     gene            15912..16718
                     /gene="pstB"
                     /locus_tag="IXT20_001333"
     CDS             15912..16718
                     /gene="pstB"
                     /locus_tag="IXT20_001333"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HAQ4859788.1"
                     /translation="MKKYNWNETNVITMPKEKEVALYTDDIHVWYGDNEAIKGVDLEF
                     EKNKITALIGPSGCGKSTYLRSLNRMNDGIANTKITGKIMYQGVDINAPQVDVYEMRK
                     RIGMVFQRPNPFSKSIYDNITFALKQHGEKNKKKLDEIVETSLKQAALWDQVKDSLDK
                     SALALSGGQQQRLCIARAIAMKPDILLLDEPASALDPISTGTVEETLVNLKEDYTIII
                     VTHNMQQAARISDYTAFFYLGKVMEYDKTNKIFTRPKIQATEDYVSGHFG"
     gene            16734..17492
                     /locus_tag="IXT20_001334"
     CDS             16734..17492
                     /locus_tag="IXT20_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HAQ4859789.1"
                     /translation="MTKEIISSKDLHLYYGKKEALKGIDLSFNQGEITAMIGPSGCGK
                     STYLRSLNRMNDLIPNVTITGSVLYKDKDIYGPKTDVVELRKEIGMVFQQPNPFPFSI
                     YENVIYGLKLKGEKDKKVLDQVVEESLKAASVWDDVKDKLHKSALSLSGGQQQRVCIA
                     RVLAVNPEIILLDEPTSALDPVSTGKIESMLLELRERYTMIIVTHNMSQASRISDKTA
                     FFLDGHLIEYNDTKKIFMNPEKQETEDYISGRFG"
     gene            17505..18182
                     /gene="phoU"
                     /locus_tag="IXT20_001335"
     CDS             17505..18182
                     /gene="phoU"
                     /locus_tag="IXT20_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010723432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HAQ4859790.1"
                     /translation="MLRTQFEEELLNLHNQFYEMGMMVSSAVHKSVRAYIKHDKILAQ
                     EVIENDININNMETRLEKKSFEMIALQQPVTTDLRMIITVMKASSDLERMADHAVSVA
                     KSTIRVKGQTRIPEIEKEISDMSDYVKKMVDNVLVAYVKTDEKDARTIANMDQRVNDY
                     FNRIYNATIKNMQENPETVISGTDYLNVASYLERIGDYVTNICEWIVYLATGKITELN
                     TNHNEIF"
     gene            18405..19436
                     /locus_tag="IXT20_001336"
     CDS             18405..19436
                     /locus_tag="IXT20_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HAQ4859791.1"
                     /translation="MQKKISIITIFFICVLTSWVIQHQAALYEVPVIEIEKVRIKEDH
                     QLITGKLMNSDQKGESIYLSVPYQKNQIETANIKARQMYLVQMHGDEWQLVAKKNDGW
                     LFFFTSIFIGAMLLIGGKQGLFALGSLIINIVLLLLLLLIFTKTEGISLLNKSILFVV
                     IGMIISILALDGWNRSSIIKISAAVTSVFLAFFICLLTMNHWKDQGLRYEELNYLTRP
                     YRSVFLSSVLIGTAGGTLDTVITVIATLEELTKRQPEITAKQLWLSGRQVGRDVIGSM
                     TNILLFVYISGSVPSLLLYLGNQWSFKETIEQHLSLEFLRVIAGSLGIVLSIPISVLF
                     FLTVRRLKR"
     gene            19433..20200
                     /locus_tag="IXT20_001337"
     CDS             19433..20200
                     /locus_tag="IXT20_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HAQ4859792.1"
                     /translation="MNVLIILVGILAISVLLIGGTQGMYILLGLFINLGIFFLLLFGY
                     HQKWPILALSVIGFLLIAVVILFFINGYNLKMRAAFVSILIFLFCFLLLLPITDFLAI
                     QGFTSVELEELSGLDKTLAIDFRLLTRSLLLISLSGAVLDASVAISSGTFEVYQANPQ
                     LSFYQLRHSSFAIAKKILASTVMTLLFAFMGSSLALILWFIDLDIPFQQVINEKSFVL
                     EYTMAILTTLSALIVLPLTCVTTAYLFTKKKEQLKME"
     gene            20300..21868
                     /gene="liaX"
                     /locus_tag="IXT20_001338"
     CDS             20300..21868
                     /gene="liaX"
                     /locus_tag="IXT20_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="daptomycin-sensing surface protein LiaX"
                     /protein_id="HAQ4859793.1"
                     /translation="MKERERILDLVKKGILSTEEGLDLLESMATEKDEKQIKKEADKV
                     TASHKEKDKASQLIDKLENGEEEISEPVDPKERERQDQENLEKILDELATEANKTSAR
                     LDEVNAQFADNKAARNEKQEALMQLNTKEELGELTEEELAQRQALEAEIKELEASGDT
                     LLEEQLKLEAELKDIRKNQWSEKKETFTDKFELPDDWKDQATDTLNQVGEKMSEAGSQ
                     LGKFLKKTFQTVSETVNDNMEWKDVSLRVPGIATTKFEHEFYYEAPAASILDIKAANG
                     NVTLKTWDSNDVKVEAKIKLYGKMGAEPFEAFSERSQIEVNEDHISFQIPNKRVRADL
                     VFYLPKRVYDHAAIKLLNGNIMIETLEAKDIYTKSTNGNIIVDQLTATMLEVEGVNGN
                     IDIRNGNILDSIIETVNGTVTFGATAENLSVSLVNGDVRLTIKEDNLKKVEASSVNGN
                     VKVALPDGIGLEGHAKTSLGSINSRLSNYEVVREKKERTNQMLQFRRVSDDEIAQVQL
                     STTTGSIYLKDTDK"
     gene            22168..22485
                     /locus_tag="IXT20_001339"
     CDS             22168..22485
                     /locus_tag="IXT20_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HAQ4859794.1"
                     /translation="MKKKLTKSNKNVVLTGTLAGIGEYIGIDPTVIRVLYVFLSLVAF
                     GSPIILYILLALIIPSARSARTNYGHNNAYYEKNNYREQTREQTKKRKEAEKIDDDEW
                     SDF"
     gene            22485..22835
                     /locus_tag="IXT20_001340"
     CDS             22485..22835
                     /locus_tag="IXT20_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HAQ4859795.1"
                     /translation="MSYFQRILVNTLTFISLAVLFPAMVHVSSIWTAVLASFVLSVLN
                     MFVKPILTILSLPFTLVTLGLFTFVINAAILKMTSFFVGAEHFSFSSFGAALLVAILM
                     SLVNVIVSEHNSRK"
     gene            23002..23940
                     /locus_tag="IXT20_001341"
     CDS             23002..23940
                     /locus_tag="IXT20_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr kinase/phosphorylase"
                     /protein_id="HAQ4859796.1"
                     /translation="MSEVVKVSQLVKKLSLEIVTGDEKSLDRVIVTGDISRPGLELTG
                     YFNYYSHNRLQLFGSKEISFAERMIPEERLIIMRRLCSEDMPAFIISRGLPAPEEMIQ
                     AANENGIAVLRSPISTSRLLGELSSYLDGRLAPRTSVHGVLVDVYGLGVLIQGDSGIG
                     KSETALELIKRGHRLIADDRVDVYQQDELTVIGEPPKILEHLIEIRGIGIIDVMNLFG
                     ASAVRGSMQVQLAVYLEAWAKDKKYDRLGSEDTTVEIAEVGIPQIKIPVKTGRNVAII
                     IEVAAMNFRARTMGFDATKTFEERLSRLIEENSGND"
     gene            23955..24785
                     /locus_tag="IXT20_001342"
     CDS             23955..24785
                     /locus_tag="IXT20_001342"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HAQ4859797.1"
                     /translation="MLAQINRVAFELVGIPIYWYALIIVSGIIIAMWLSSREAVRVGL
                     KADDVTDFMLWGLPISIIGARLYYVLFDLPQYIADPIQIFNIRSGGLAIYGGLIAGAI
                     TLYFFTKYHFISLWTFLDIAAPSVLLAQAIGRWGNFMNHEAYGPDTTRSFLESLHLPQ
                     FIIDNMYIEGIYRQPTFLYESLWSLIGFILLLFLRKKPHLLKKGEVLLCYLMWYSFGR
                     FFIEGLRTDSLYFLGVIRISQLLSAVLFVGTLILFIWRRKKGNLPDYDRSLGKNQQII
                     "
     gene            24829..25854
                     /locus_tag="IXT20_001343"
     CDS             24829..25854
                     /locus_tag="IXT20_001343"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HAQ4859798.1"
                     /translation="MNYRVAVLGPGSWGTALAQVLAENGHDVRIWGHRASQVEEINTQ
                     HTNQRYLPDHYLPDSIQAFTNMEDAVKDADAVLFVIPTKAIRSVAKELVPKLNTKPVI
                     IHASKGLEQGTHKRISDILLEEIPAEKRKAVVVLSGPSHAEEVAKHDITTITAASTDA
                     KAAKYVQELFMNNYFRIYTNPDVIGVEMGAALKNIIAIGAGAIHGLGFGDNAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGIGDLVVTCTSVHSRNWRAGNLLGQGQPLEEVLA
                     NMGMVVEGVNTTQAAMELAQMMEIEMPITQTIYEVLYEQKEIKTAAKEIMLRDGKKEN
                     EFSLTIF"
     gene            25869..26807
                     /gene="galU"
                     /locus_tag="IXT20_001344"
     CDS             25869..26807
                     /gene="galU"
                     /locus_tag="IXT20_001344"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HAQ4859799.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LKSGIEDILIVTGKAKRPIEDHFDSNIELETNLSEKGKTELLKLVEETTDVNLHFIRQ
                     SHPKGLGHAVLQARAFIGNEPFVVMLGDDIMEDEVPLTQQLIDDYERTHASTIAVMQV
                     PHDETSKYGIINPGEVLEDGLYNVKNFVEKPDPSKAPSDLAIIGRYLLTPEIFDVLSE
                     QQPGAGNEIQLTDAIDTLNKTQRVFARKFTGKRYDVGDKFGFMKTSIEYGLTHPEVGA
                     PLREYIMSLGKTLEAEGSKINHNPKGITEIKEEKKK"
     gene            26932..27372
                     /locus_tag="IXT20_001345"
     CDS             26932..27372
                     /locus_tag="IXT20_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HAQ4859800.1"
                     /translation="MSGGEIAGLIAAIAFAILVVFIVRVLLQVSKTLEKVDQTVAEAN
                     TTIEIVTQDVDLLSRQVEGLLVKSNELLLDINGKVATIDPLFTAVADLSESVSELNHS
                     SKNIVTKVGGIGKTTAKATVAGKVGGTAMKFFKNKKHTETTGGK"
     gene            27375..27977
                     /locus_tag="IXT20_001346"
     CDS             27375..27977
                     /locus_tag="IXT20_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HAQ4859801.1"
                     /translation="MAKKGGFLLGAVVGGTAAAIAALLLAPKSGKELRDELAAQADDL
                     MDMASDYTDMAKEKSSELTGIAKEKAQGLSQQAGDLAGNVKQKVSESAEEAGSESDDI
                     LLNLKEQSADLSNRFKKTAEDLKKPADELGSIAKDTSEDIMIDVKDAADKAGETVNEG
                     MSEAKPVADDTKEMLKMAKEDAKEAKKEMKDTMTDSTTNH"
     gene            complement(28040..29143)
                     /locus_tag="IXT20_001347"
     CDS             complement(28040..29143)
                     /locus_tag="IXT20_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HAQ4859802.1"
                     /translation="MNNVKINELRQWMAQHQVELAYISDPGHIAYFSGYESDPHERVL
                     ALFIPLEQDPFLFTPALEVEDAQNSSWAYDVRGYLDSENPWEIIGKELRARYGQPGNV
                     GIEKNALTVDRFDAISDILTGTDSYTDLTSIIQRLQLIKTVEEKDKLIEAGKWADVAF
                     EIGFKTVKEGVTEQAIIAEIEYQLKKQGVSQMSFDTLVLAGANAASPHGTPGSTKVSP
                     NELVLFDLGVIWNGYCSDATRTVAYQKPTEFQEKIYNITLEAQLAAQEAVRPGVTAGE
                     LDQIARNVINSYGYGEYFNHRLGHGIGTTVHEFPSLVEGNDLVIEEGMCFSLEPGIYI
                     PEKVGVRIEDCVYVTSDGCVPFTTTPKELLVLE"
     gene            29353..30354
                     /gene="ccpA"
                     /locus_tag="IXT20_001348"
     CDS             29353..30354
                     /gene="ccpA"
                     /locus_tag="IXT20_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite control protein A"
                     /protein_id="HAQ4859803.1"
                     /translation="MEKQTITIYDVAREANVSMATVSRVVNGNPNVKPATRKKVLEVI
                     DRLDYRPNAVARGLASKKTTTVGVIIPDVSNMFFASLARGIDDVATMYKYNIILANSD
                     GNDQKEVNVLNNLLAKQVDGVIFMGHHITDEIRGEFSRSKTPVVLAGSIDPDEQVGSV
                     NIDYVSATKDAVNKLAKSGNKKIAFVSGALIDPINGQNRLKGYKEALSENNLPYSEGL
                     IFEAQYNFKDGLSLVDRIHNSGATAVYVSDDELAIGILDGLLDRGVKVPEEFEIITSN
                     NSLLTEVARPRLTSITQPLYDLGAVSMRLLTKMMNKEEVEEKTIILPYGIEEKGSTK"
BASE COUNT        10064 a         5311 c         6587 g         8585 t
ORIGIN      
        1 ttagttcgag agctcttttt tttgctttgc taccttgttc cttgaagatt ctgctgaatt
       61 aagatagagt taagttaact acaagaattg gagggatcca catgaaatgg aaagaaaaag
      121 tagacgaagc agctgaaaag ttatatgatt tggtcaaatc agaaaaatac aatattgaag
      181 tgaatattcc gaaaaaaggc ggaaaagcgg tccaggtaaa atcaaaacgc ccaacgaatc
      241 atacaaaaaa atggatggct aaaaaccgct aataataaca aaagatccat aagtcggcat
      301 tttcatcgat tttagggtct tttgttattt tctaggttga acatatctaa aaaattgttt
      361 ctgacaatag gagtagggag aacgatgtgt gtctaaatcc tctgttgatg gatagaaacc
      421 tttatctttt tcagttgtga atcaggtata taccagttat aatagaagaa acgaaaggac
      481 gggtgtacgt tatgtcgaat caattacctg tatatataca gattcatgac caattaaaaa
      541 atgaaatcga acagggtata tggaaagtcg gagaccgtct tccttcagaa agagaattag
      601 cagtgaaatt caatgtcagc cgtatgacgt tgagacaagc aattcaaaac ttggcagatg
      661 aaggaatctt agaaagaaag atcggttcgg gtacgtatgt tgcgcgagaa aaagtacaag
      721 aaaaaatgtc tggtgcaaca agttttacag aaattatgga atcacaaaat cgagtaccgt
      781 ctagtcggac aatctcttat ttcttgactt cgccaagctc aagtgaaatg gaaaaactaa
      841 acttgagtaa agatgacacg atcttgcgaa tggaacgtat tcgtttcgca gatgatgtcc
      901 ctatttgttt tgaagttgcc agtgttccac aaaagctgat cgatggctac agcaaagcag
      961 agattactga atcgttgtat cgcacgctag aagaaaaagg cgggaaaaag atcggtccag
     1021 cgaatcaaac gattactgct gtgttggctt ctgagaaaat tgctgactat ttagaggtaa
     1081 aacgaggaga tgcagttctt cgtttgcgcc agatttctta ttttgaagac ggtacgccgt
     1141 ttgagtatgt gcgtacgcaa tacgtaggaa atcgttttga gttttatttg gaaaaataaa
     1201 aaacaggtga tccctgattc cttattagaa ataaaagaga atcagaatca cctgttttta
     1261 tatatgatca gttatcagtt gctcgtgata agaaacagaa ccctcttcaa attggacttg
     1321 tccgttcagc agctcaatga tggattcttt aaagtggtca gcactttttt catctacagc
     1381 acagatgacg ctaacttgat cagtatattg gatttctttt ataaaaaaat gttggagttc
     1441 aagataattc tgtaattttc ctagttgcgg atacgtgatc atcacagaga tctcctgttg
     1501 caaggtacct tctacgattc caatgtcaga caatgcttga gagaccgcat gagtgtatgc
     1561 tcgtattaat ccgcctgtgc cgagtttcgt tcctccaaaa tatcgagtga ctactgcagc
     1621 tacattgatc agttgatttt ttttgaggac ttccaacatc ggtacgcccg ccgttccact
     1681 tggttctcca tcatcgctgc ttcgctggat ttcatttttt tctccaatga tgaaggcgct
     1741 gcagttatgg ttcgctttcc aatgttcttt ttttatttct tgaatgaaac tttttgcttg
     1801 gtcctctgat tctattcgtc taagagaaca gataaagcgt gattttttga tctcaatctc
     1861 actttgtcca ttttctttta ttgtacgata gcgctcgatc atcttgtcac ctcttttttt
     1921 cattttcttt attcagtata aataaagttc taggttgttt caacactgta acggttgcgc
     1981 aattgctgta gtatgcgata ataaagacaa aggcaggtgg atctggggat gcaggaacga
     2041 atgaaaaagt acgatgcaat tacgcattat ttgaagaaca atggcggctc tcaagtaacg
     2101 ttgaccttta cccaatttga cgaactgctg tttcccagta acggacttcc caaaacagca
     2161 agggaaagta cagattggtg ggcaaatgat tataaacacc ctgaaaaagg tgcctatggc
     2221 tggataaatg caggctatga agtagttgtc atcaatttgg acaaagaata tgtcgtgttc
     2281 aataaattag tgaaatctag ttggctattt gattaaataa accgcgttta gaaaacgttg
     2341 acaaatttct ttctttttat ataatagagt aacagacgcg aagaaaacgt ttgcatagtt
     2401 tgttttaaaa aggagagaaa agtatggcta aaaaaacaat catgcttgta tgttcggctg
     2461 gtatgagcac aagtttacta gtaacaaaaa tgcaaaaagc tgctgaagca aaaggaatcg
     2521 actcggatat tttcgctgtg tccgcttctg acgcagataa caaccttgca aataaagacg
     2581 tggatgtgtt gttattaggc ccacaagttc gctttatgaa agctgatttt gagaaacgtc
     2641 tggaaccaaa aggcattcct ttagatgtca tcaatatggc tgattatggt atgatgaatg
     2701 gcgaaaaagt gttagatcaa gcaatcacgc tcataggtga ataaatagaa cagtataact
     2761 atcgaacaag cgatccaaaa aatagatcgc ttgttttttc gtatattttt ttagggcgaa
     2821 gaatatgaat catttttggg gtagaaggct tctgacgaga gatattgaag caatagactg
     2881 tgaggaggta aatttaccaa attatgagaa gatcgcagca gtggaacgtt tgggcaactg
     2941 gattctttgt catcgctgtg gaatgaggac gtccgtcatt gatggacagc ttgcagatta
     3001 cggttatttt tgtacacatt gtctatattt agggcgatgc gacactcaac aagatttata
     3061 tttgtttgat cagcctgaag caaaacctcg agaagtcgtc tttttttgga caggacagct
     3121 tactgaaaaa caaacggaaa tctcaaagag aatactttgc cattcagaaa aaagacatca
     3181 tcttatttgg gctgtaacag gtgcagggaa aacagagatg ctttatccta ttttagtgga
     3241 aactttaaaa gctggaggaa gagtagcgat ttgtacaccg cgagtcgatg tttgtaatga
     3301 attgttttta cgctatcgtt atgtttttcc agaagaaaaa atcacgttgc tccatgggaa
     3361 tacagcaacg gcttatacct ttacttcttt tgttatctgt acgattcatc agctataccg
     3421 tttttatcgg tcctttgatt taatagtgat cgatgagatc gatgcgtttc cttataacgg
     3481 ggatgcagga ttagctcatg ctgtgcaaac agcgattaaa ccagacggac gattcatcta
     3541 cttaagtgcc acacctgata aaaagttact aaaggaaatc aagcagacgt ttgacatcca
     3601 caaacttgct ttacgctttc atcggcgttt gcttcctgaa cccgtcttgg ttttcatgaa
     3661 taattggcga caaaaatgca agaacaagaa gaagataaaa tctttggtgc ggatcattca
     3721 tcaattaatt aaagaaaacc atatcctttt attttgtcca agtatcacga tgatgcatcg
     3781 tttgaaagcc acacttcaag aggctcttcc gagatatgag atcacagatg tttcttcaag
     3841 agacaatcag cgggcggaaa aagtccagaa aatgcgagaa gcaaaatatg atatactgtt
     3901 aaccacgatg atccttgaac gtggagtcac atttgaaaga atatccgtta tcgttttagg
     3961 agcggatcat ccggttttta ctaaatcttc tttggtacaa atcgcaggaa gagccgatcg
     4021 taaagggcct tatacgaaca gtcaagtata ctttttctat gaagaaaaaa ctgcagctat
     4081 tcgaaaagct tgtcaggaaa tcaaagaaat gaatgaagaa gggaagaaga tgctgtgagg
     4141 tgcagttgct gcagtaaaga tatcttccaa aatttaacat tcagcgagat tattggtgtt
     4201 tcgaaataca gattacctca gacttgttca gtttgtcgcg cacaatttca tttattggaa
     4261 acaagaggct gttctggctg tcaaaaacca atccatttca atctagagag tacgaaaaag
     4321 aaagtaaagt ctattcccgt ttactgcagt gactgtcaag aatggaagaa gaaatatccg
     4381 acctatgatt ttcagcaccg tgcctttttt gtgtacgatg aaaatatgaa ggcctggctc
     4441 aatcaataca agtttatggg agatatccga ctagcaggca cctttgcata taaatggacg
     4501 cagataaaaa aagaataccc gacatacatc tattgtccaa tacctttatc tgaaaaaagg
     4561 cgaacagaaa gaggattcaa tcaagtaagc gagatgttga aagcagcaag aattcctttt
     4621 cagatgctac tcaaaagaga gaagcatctg ctggctcaag caaaaaacac gcgaaaggaa
     4681 agattggaga tgcctcaacc ttttgaactt gcagtcacct tttctaagat acaaaataaa
     4741 aaaatattga ttattgatga tgtctataca acaggacaga ctcttttcca cgcagcagat
     4801 tgtttgcagg tcgcacagcc gaaaatcatc cgaaccattt ctttggcgag atgaattaga
     4861 tgtggaaatt tgatagcgat ttaatttgca aataacctag agttcgatat aatagaatta
     4921 agaagaggga aagagtggtc cttctccttc ttgattgtgg tagacgaaag gggtaatttt
     4981 tatgtttaga tataatgtac gcggcgaaaa tatcgaagta actgaagcga ttcgcgacta
     5041 cgttgagaaa aaagtaggca aactagaacg ctatttcagt gacgcacccg atgcaacggc
     5101 gcatgtgaac ttaaaagttt atacagaaaa aacagcaaaa gtggaagtaa cgatcccatt
     5161 accttatctt gtattacgtg ccgaagaaac ttctccagat ttatacgcaa gtatcgattt
     5221 ggttgttgat aaattggaac gccaaatccg caaattcaaa acaaaaatta atcgtaaatc
     5281 ccgtgaaacg ggcatgaata ctgcagatgc tgctgcttta ttcaatgaag aggacgaaaa
     5341 cgaaaatgat tctgaattag atatcgtacg tacgaaacgc ctttcattga aaccaatgga
     5401 tagtgaagaa gcagtcttac aaatgaacat gctgggacat aacttcttta tttttgaaga
     5461 tgcagaaaca aatggtacaa gtatcgttta ccgccgtaaa gatggaaaat acggactgat
     5521 tgaaacagac tgatcagcct tacttaaata acaacgaaaa cttgcagcag ctactgacag
     5581 ctgccgcaag tttttttgag tttcctcctc ttttagatga aatatgaata aattatgaag
     5641 ttcttacttt aatacgctta agatatttca attatctata actaatgata aaatgaaaag
     5701 taaagtctac atagtctgga taatacacag aaggaaaagg agattacaca acgcatggcc
     5761 aatttgattc gttcacttat cgaaaacgat aagaaagaac taaaacgttt agatagcatt
     5821 gcaaaaaaag ttgaagcata cgctgaccaa atggcagcat taactgatga acaattacag
     5881 gcaaaaactg ctgaattcaa gggcagatac caaaaaggag aaacattaga tcaattactt
     5941 ccggaagctt ttgcagtcgt ccgagaagca gcaaaacgtg tattggggtt atatccatac
     6001 cacgtacaat tgatgggtgg aatcgtgctt cacgatggaa atatccctga aatgcgtact
     6061 ggtgaaggta aaaccttgac cgcaacgatg ccagtatatc taaatgctct gactggcgaa
     6121 ggggtacacg tagttaccgt caatgaatat ttagcaaccc gtgactcgac ggaaatgggc
     6181 gaactttata atttcttagg attaacagtg gggttgaata tcaactcaaa atcttcggat
     6241 gaaaaacgcg aggcttataa ttgtgatatc acttatagta cgaataatga attaggattt
     6301 gactacttga gagacaatat ggtcgtttac cgccatcaaa tggtacaacg tccgctgaat
     6361 tatgccattg tcgatgaagt tgactcaatc ttgattgatg aagcgagaac gccgctgatc
     6421 atttcagggc aagcagaaaa atccacagct ttatatacaa gagcagacaa cttcgtgaaa
     6481 cgtttgaaag aagaagaaga ttataaaatc gatgtacaat caaaaacaat cagtttaacc
     6541 gaagcaggga tcgaaaaagc agaagaaaca ttcggtttgg ataacttata cgacattgaa
     6601 aatactgcat tgacccatca cttggatcaa gcattgagag ccaactatat catgctttta
     6661 gatatcgatt atgttgtcca agacggaaaa gtactgattg ttgaccagtt cactggtcgg
     6721 atcatggatg gacgccgtta ttcagatgga cttcaccaag caatcgaagc taaagaaggc
     6781 gtagagatcg aagacgaaac gaaaaccatg gcgacgatca ctttccagaa ctatttccgt
     6841 atgtacaaga aacttgcggg aatgactggt acagctaaaa cagaagaaga agaattcaga
     6901 gaaatttata acatccaagt ataccagatt ccgacaaatc gtccggttat ccgtgatgat
     6961 cgtccagatt tactttaccc aacattacaa agcaagttcc acgctgtcgt gcaagatatc
     7021 aaagatcgct accgcaaagg tcaacctgtg ttagtcggaa ctgttgcggt tgaaacatct
     7081 gagttattat cagatatgct gaatcaagaa cgtattcctc atgaagtgct gaatgcgaaa
     7141 aatcacttta aagaagcaga gatcatcatg aatgctggtc aaaaaggtgc tgtgacgatt
     7201 gctaccaaca tggctggtcg gggaacagat atcaagttag gacttggggt aagagaactt
     7261 ggtggtctag cagttatcgg gacagaacga catgaatctc gtcgtatcga taaccagctt
     7321 cgaggacgtg caggacgtca aggagaccct ggtatgtcac aattctactt gtcattggaa
     7381 gatgatttga tgaaacgatt cggttcagaa cgaatcaaag ctttcttaga tcggatgaag
     7441 atcggtgagg aagatgctgt tatccaaagc aagatgctga cgaaacaagt agaatctgca
     7501 caaaaacgtg tagaaggaaa taactatgac acacgtaaaa acgtcttaca atacgatgat
     7561 gtgatgcgtg aacaacgtga agtaatctat aaacagcgac aagaagtaat catggaagaa
     7621 aaaagcttat ccaagccact aatgaacatg gtcaaacgga caatcagtcg tgtagtagat
     7681 gcgcatacac aattagagaa agaaaattgg aatttggaaa gtatcatcga ttttgccggc
     7741 agtactcttg tccatgaaga tagtattact cttgcagata tcgaaggaaa aacatctcag
     7801 gagatcaaag agtatctaaa cgaacgagca caagaagtat ttaataccaa agcggctcaa
     7861 ctaaatggtc cagagcaact gttggaattt gaaaaagtcg tgatcttgcg tgttgtggac
     7921 tctaaatgga cagaccatat tgatgcaatg gatcaattac gtcagtcaat cggcttacgt
     7981 gcttatggac agaacaatcc attagtcgaa tatcaaacag aaggctataa gatgtttgaa
     8041 gatatggtag gcgccatcga gtatgaagtc actcgaatct ttatgaaagc tgagatccgt
     8101 caaaacgtac agcgtgaaca ggtttctcaa ggaacagctt ctcactcaga agacggagat
     8161 gccaaagaaa atagtaatac tagcgcgaaa aaacagccaa tccatgtgaa caaggtcgga
     8221 agaaatgatc cttgtccttg tggaagcggg aaaaaatata agaactgtca tggaaaaaat
     8281 gcaaactaat ttctgacagc aggattggtg agacaaattc tttttttaag aattcgtctc
     8341 accttctttc tgttatttgc tgattctgtt agactagata taatgaaaaa agtatagtga
     8401 aagaaggaaa aaacatggaa atatccgaaa ttcgaaatct cctgacagaa atgcaggaaa
     8461 aggtcacaag tttcaggagg tctctttgac ttagatcagc tggaagaaga catcgcggaa
     8521 gcagaagcaa aaatggctga acctggtttt tgggatgaca gcgaagccgc acaaaaagtg
     8581 atcagcgaaa ataacgctaa taaagaaacc tatgaccaat tccattctct tgccgaagaa
     8641 ctagaagaaa tcgaattgat ggtggagatg ctgcaagaag aacctgatgc agcaatgcaa
     8701 gaagaagcag aaacacgtat caatgcatta gatgaaaaaa tgaagacgta tgaattatct
     8761 atgctgttag acggacctta tgatcgaaac aatgcgatcg tcgaattgca tccaggggct
     8821 ggcggaacgg agtcccaaga ctggggcagc atgcttttac gtatgtatac acgttgggca
     8881 gagcagcatg gctatcaagt agaaacacta gattatcaag caggtgatga agcagggatc
     8941 aagagtgtga ccctgctgat taaaggacat aacgcttttg gttatctaaa atcagaaaaa
     9001 ggtgttcatc gccttgtacg gatttcgcct tttgactcag cgaaacgacg tcatacttct
     9061 ttttgttcgg tagatgtgat gccagagctt gatgaaacga tcgatataga aatcaatcca
     9121 gatgatttga aaatcgatac gtaccgagca agtggtgcag gtggtcagca catcaataaa
     9181 acagaatctg ctgtacggat cactcacttg cctacaggaa cagtagtagc tagtcaggca
     9241 caacgctcac aattgaaaaa ccgtgagcaa gcgatgggga tgttaaaagc taagctttat
     9301 caattagaag tggaaaagaa agaacaagaa gcagctgcac ttcgaggaga acaaaaagaa
     9361 atcggctggg gttcacagat ccgttcttat gttttccatc cctattctat ggtaaaagac
     9421 catcggacaa acgtagagac aggaaatgtc caagcagtta tggacggtga ccttgattta
     9481 tttattgacg gctatttaaa aatgcatcta caataagaac tcattgttaa attgaaataa
     9541 aattgtaaga ttttgcaagc atgttgaaac attgccatgc tataatagct aaagactgaa
     9601 aaagatatgg agagaacgcc atgattgaaa tgaaggatgt aatgaagaag tactcaaatg
     9661 gtacgaccgc tatccgcaat ctctcgattg tcatcgaaca aggagagttt gtctatgtgg
     9721 ttggtccgtc tggagccggt aaatctacat tcatcaaatt gatgtatcga gaagaaaaag
     9781 cttcaaaagg aactttgaac gtcgcaggtc atgatctaat agcaatcaaa aaccgcgaag
     9841 ttccttatct acgcagagaa atcggcgtgg tgttccaaga ctataaatta ttaccaagaa
     9901 aaacagtata tgaaaatgtt gcgtacgcta tgcaagttat cggcagaaaa cctcgagata
     9961 tcaaaaaacg agtaatggaa gtgctggacc ttgttggttt gaaacacaaa gttcgagtct
    10021 ttcctagcga attatccggt ggggaacagc aacgtgtctc tattgctcgg gcaatcgtga
    10081 atacgcctaa agtacttatc gcggatgaac cgacaggaaa tctggatcca gaaaactcat
    10141 gggaaatcat gaaattatta gatcggatca atgcccaagg aactactgtc gtgatggcta
    10201 cacataacag tacgatcgta aatacgattc ggcaccgagt aattgctatc gaaaacggcc
    10261 gcattattcg agatcaagcg gaaggggaat atggatacga tgattagaac ctttttccgc
    10321 catttattag aaagtatcaa gagtttgaaa cgtaacggct ggatgacagt tgcttcagtc
    10381 agtgcggtaa cgatcacgtt gactttagtc ggtgtattca tggcagttat catgaatgca
    10441 actaaattag ctcaggatat agaaggaaac gttgatgtat ctgtattcgt agatatcggt
    10501 acaaaagacg aagagatgaa aaagctagaa aaagaattga atgacttaga tcatgttaaa
    10561 aaagttgaat tttcaagcaa agaacaggaa ttgaaaaaaa tccaaaacga aatgggtgat
    10621 tcctggaaat tgtttgaagg tgacaataac ccgttatatg atgtgtatgt agtaagtgct
    10681 actgatcctt cttatacaaa acaagtagca gaagaagcag cagatttgaa gaatgtttct
    10741 cgttctgatt acggtggagc ttcctcagat cggattttcc aaatcgctgg tgcagttcgg
    10801 acatggggat tagttgcagc ggcattgctg ctgttcgtag cgatgtttct catttcgaac
    10861 acgattcgga tcacgatttt gtctagacag cgtgaaattc aaatcatgcg tttagtcggt
    10921 gcaaaaaatg gatatattcg ctggccgttt ttccttgaag gtggctggat cggtctgatc
    10981 ggtgcgattc ttccgatttt gatcatcaca tttggttatg catggttttt ccaattgatc
    11041 aatccatcat tattacgttc ccattattca ttgattcgtc cacaaaatat cgtttggaaa
    11101 atcaatcttt taatggtcgg cattggtgtg atcattggtt cacttggctc gatcatttct
    11161 atgagacgtt ttttgaaatt ctaataccac aatataaata aaatgagaaa gttggacagt
    11221 ttcgttccag ctttcttttt ttattggcta ggagaataga aggaagcggc ttttttccct
    11281 ttactttaaa aaacaacttg ggtattctta agttggtaga agaagaggtg aagccgtgaa
    11341 aattatcgtt gacaattttt ttacgatcct tttgattatg aatattttgc tttcctttat
    11401 catcgttttt cgagaaagaa aggaaactgc tcaaacatgg gcatggctgc tcgttttgat
    11461 gttcattcca gttgtaggat ttattttata tattttttta ggaagaggga tttccaaaga
    11521 gaaaatcttt gatttgaaga tccaagggaa aatcggtaag aacctggaaa ttgaagaaga
    11581 caaacaggca ttgatgcgag ggctttatcc tcatccgcct actggacaag tagatgtgaa
    11641 acaattgatc tatatgctca cggtatttga aagtacgctc tatacgacta aaaatgaaat
    11701 cactttattt acagatggtc gtgaaaaatt cgatgcgctg attgaagata tcaagcaggc
    11761 agaagaccat atccacttcc agtattatat ttatcgcagt gatgcattag gtgaagaagt
    11821 tcgagatgca ttgacagagg cggcaagacg aggggtaaaa gtcagagtcc ttcttgatgc
    11881 atggggttcc acacaagttt cttccagctt ttttcagaat ttgaaaaaag caggcggaga
    11941 agtcgcattt ttctttcctc tatttgttcc gtatattaat cctcggatca attatcgcaa
    12001 tcatcgtaaa atcgtagtta ttgatgggaa aatcggatat acaggtgggt tcaatgttgg
    12061 aaatgaatat ctaggattgg taaagaaatt tggttattgg cgagacaatc atttgcgtat
    12121 ttacggagaa gcggtgtata ccttgcaaaa ccgttttttg atggactgga attctcagca
    12181 tacaaaagaa gtaggctatt ctcccaaatt tttcccgtcc attcacacta ctggtgatat
    12241 agctgttcaa atcgtgacaa gcggaccaga taccgaacat gaacaaatca aaatgactta
    12301 tttgaaaatg atttcgttag ccaaacggga gatattgatc caaacgcctt attacatacc
    12361 ggatggatcg attcatgaag cactgaagtt ggctcttctt tcaggcgtaa aagttcatat
    12421 ccaaatacct aacaaaccag atcatcttct cgtttactgg gcaacgtatt ctttcgctgc
    12481 agagctgatc gagtatggag caaggatcga aacatatgag aatggattta ttcatgcaaa
    12541 gacgatgatt atcgatggtg gcattgtttc agttggctca gcaaatatcg atgttcgttc
    12601 atttcgcttg gactttgagg tcaatacact ggtttatgat gaacgaatgg cttcacgtgt
    12661 aagaaaagct ttttttgaag actcgaaaat ttctacgcat ctcacaaaag aaatgtatga
    12721 aaaccgagga ataatcatca agatgaaaga aggattagca agattgattt cgccgctcct
    12781 ttaaaacata aaaaggacat gagaaccatc aacagatgga gatctcgtgt ccttttctaa
    12841 cggggccatt tctgagagta cgtcagaaat ggagaaagcg tctcgcatga gcgaaattaa
    12901 ctttggggta tctcttgttt tttggggaaa gcaagaaata agctaatata tgctattaaa
    12961 agagacacat ttcaaagcca gtatagcata gaaactttta atgagatagt tttttttaca
    13021 attatgagaa tttattttgt tatttaatga ttttgattcg ttttcatcaa gtaatgaaaa
    13081 tgataaaata aacatacttt aggattaaag gagatttcta atgaaaaaac ttaaattatt
    13141 cttaccaata ataggattag ccattctttt atcaggctgt tcaaagtgga ttgatcgagg
    13201 agaatccatt acggctgttg gctcttctgc tttacagcct ttagtagaaa cagttgctga
    13261 aacttaccag acagaaaatc ctggaaaatt catcaatgta caagggggcg gaagcggaac
    13321 tggtttgtct caagtacagt caggagcagt cgacatcgga aactctgatt tgtttgcgga
    13381 agaaaaatcg gggatcgatg cctcagcatt agtcgatcat cgagtagcag ttgttgggat
    13441 cactcctatt gtgaacaagg atattggcgt aaaagacatc agtatggaaa acctgaaaaa
    13501 actatttaca ggcgagatca aaaactggag tgaagtcggt ggaaaagatc agccagtagt
    13561 tatattgaac cgcgcttcag gaagcggaac ccgtagcacg tttgaaaaat gggtattgga
    13621 cggagaaaca gctgttcagg cacaagaaca agattcaagc ggaatggtgc gccagatcgt
    13681 tgccgacaca cccggtgcaa tcagctatgt tgctttttct tatgtttccg atgatgtaga
    13741 tacgttgaat gttaacggtg tagaaccgac agatgaaaat gtgacaacga acaaatggac
    13801 gatttgggca tatgagcata tgtacacaag agatcatcca aaaaaactga caaaagaatt
    13861 tttagactat atcttatctg atgaagtaca agataacatc gttggagaac taggttatat
    13921 ccctgtttct catatgaaag tcgaacgtga ttggcaagga aatatcgtta aagagtaatc
    13981 tttacacaaa atttacaaac ttagctaaac ttttacatgt tattaaaacc aaattgatgt
    14041 tcaatttgta cactgagtga gtatgttaaa cctgattaag ggggagtcat ctgtggaaga
    14101 tgtgaaaaaa gtattattaa caaaatcaaa acgttcaaaa atggaacagc gtggaaaatt
    14161 catcagtttt ttatgtatcg cgctgatcgt gttggttgta ttatctattt tttattttgt
    14221 agctagtaaa ggattggcca ccttttttac tgataagatc aatgtatttg attttctgtt
    14281 tggtacagat tggaatccaa gccaaatcgg agcaaatggg aaaccaatgg taggggcatt
    14341 gcctatgatc acaggttcgt ttattgtgac ctttttatct gctatcgttg ccacgccatt
    14401 tgccattggt gcagcagtat tcatgacgga gatctctcct aaaaacggaa cgaagatctt
    14461 acagccggtc atcgagctgc ttgtaggtat tccttctgtc gtttatggtt ttatcggttt
    14521 atcagttatc gtgccttttg tcagaagtat ttttggcggg acagggtttg gtatcttagc
    14581 tggtacattt gtgttgttcg ttatgatctt gccgactgtc acaacgatga ctgttgatgc
    14641 attaaaggca gtaccacggc actatcggga agcttcatta gcgcttggtg ccacaagatg
    14701 gcaaacaatc tacaaagtcg tgctacgagc agccgtgccc gggattttaa cagcagtcgt
    14761 ttttggtatg gctcgtgcgt ttggtgaagc cttagctatc caaatggtca tcggtaatgc
    14821 agcattgatg ccaacaggac ttactactcc tgcttctaca ttgacgtcca tcttaacaat
    14881 gggaatcgga aatacaatca tgggaacagt tgaaaataat gtgttatggt cattagcgct
    14941 gatcttgcta ttgatgtcat tattctttaa tatcgtgatc cgtatgattg ggaagaaagg
    15001 agagctgaaa taatgaacgc aaaacgctca gataaaattg ctacaggtgt tttatatata
    15061 gtttcgggga tcatcgtatt gattttagca gctttactac tttatatctt agttcgggga
    15121 ataccgcaca tttcatggtc cttcttgacc gaaccttcca aagcctacca agaaggtggt
    15181 ggaatcggga tccagctatt taactcgatt tatttgcttt tgatcacgat gatcatcagt
    15241 tttcctattt ctttaggtgc gggtatctat ctttcagaat atgctggaaa aaactggcta
    15301 acagatgtga ttcgcacgtc gattgaaatc ttgagctcgt tgccttctgt cgttgtcggt
    15361 ttattcggtt tcttggtttt cgttattcaa ttccagtatg gtttctcgat tatttctggt
    15421 gcattggctt tgactgtatt caacttgcct cttttgacta gaacagttga ggagtcttta
    15481 caagcggtcc attacacaca acgagaagca ggattggcct taggtttgtc acgctgggaa
    15541 acagtgatga aagtggtggt tccagaagcg acaccaggga ttttgactgg tgtgatccta
    15601 agttctggaa gaatttttgg tgaagcagct gcattgatct atacggctgg acaaagtgca
    15661 cctgcgttag attttacgaa ctggaatcca ttaagtgttt caagcccgat cagcattttc
    15721 cgtcaagctg aaacattagc tgtccatatt tggaagatca atacagaagg cacgatgcct
    15781 gatggtgttt ctgtttcggc tggcgcttct gccgtgttga tcattgctgt tttactgttc
    15841 aacttcggcg ctcgtgcgat tggcaaacga ttataccgaa aaatgacttc agcctaagga
    15901 gaagaagcaa aatgaaaaaa tataactgga atgaaacaaa tgtcatcacc atgccaaaag
    15961 agaaagaagt agcactatat acggatgata tacatgtctg gtatggcgac aatgaagcaa
    16021 tcaaaggagt ggatctggaa tttgaaaaaa ataagatcac tgcactgatt ggtccttccg
    16081 gctgcggaaa atctacgtat cttcgttcgt tgaatcgaat gaatgatggg atcgctaata
    16141 cgaaaatcac tgggaaaatc atgtatcaag gcgtagatat taatgcgcct caagtggatg
    16201 tttatgagat gcgcaaacgg attggcatgg tttttcaacg tcctaatccg ttcagcaagt
    16261 cgatttatga caatattaca tttgctttga aacagcacgg cgaaaaaaac aaaaagaaat
    16321 tagatgaaat cgttgaaaca agcttgaaac aagctgcctt gtgggaccaa gtaaaagaca
    16381 gcttggacaa aagtgccttg gctttatctg gcgggcaaca gcaacgttta tgcattgctc
    16441 gtgcgatcgc gatgaaacct gatatcttgc tgctagacga gccggcaagt gctttggacc
    16501 ctatttcgac aggaacggtt gaagaaacac ttgtaaactt gaaagaagac tatacgatca
    16561 tcatcgtgac gcataacatg caacaagcag ctcgtatcag cgactatact gcctttttct
    16621 atctaggaaa agtaatggaa tatgacaaaa ctaacaagat atttaccaga ccaaaaattc
    16681 aggcaaccga agattatgtg tccggtcatt ttggttaaga cggaggcctc aacatgacaa
    16741 aagaaattat ttcatctaaa gatcttcatc tgtattatgg aaagaaagaa gcactaaaag
    16801 ggatcgatct aagtttcaat caaggggaaa tcactgcgat gatcggtcct tctggttgcg
    16861 gaaaatccac ttatctgcgt tctttgaacc ggatgaatga cctgattccg aatgtaacga
    16921 tcaccggaag tgttttatat aaagacaaag acatctatgg tccaaaaaca gatgttgttg
    16981 aactaagaaa agaaatcggc atggtattcc agcagccaaa cccatttcct ttttctatct
    17041 atgaaaatgt catctatgga ttgaaattaa aaggggaaaa agataaaaaa gtccttgatc
    17101 aagtagttga agaaagcctg aaggcagcgt ctgtttggga tgatgtgaaa gacaaattgc
    17161 ataaaagtgc tttatcttta tccggcgggc agcagcagcg tgtatgtatc gcacgtgttt
    17221 tggcagttaa tccggaaatc atcttattgg atgaaccaac aagtgcgctt gatcctgttt
    17281 ctactgggaa aatcgaaagt atgctgttag aattaagaga gcggtatacg atgatcatcg
    17341 tgacccataa tatgtctcaa gcttcacgta tttcagataa gacggcattc tttttagatg
    17401 gtcacttgat cgaatacaac gatacgaaga agatattcat gaatccagaa aaacaagaga
    17461 cagaagatta tatttcaggt cgatttggat aaggaggaaa cggaatgcta cgtacacaat
    17521 ttgaagaaga attgctgaat ctccataatc agttctacga aatggggatg atggtgagca
    17581 gcgccgtgca caagtcagtt cgtgcataca tcaaacacga taaaatctta gcccaagaag
    17641 ttatcgaaaa cgatataaat atcaataaca tggaaacacg cttggagaag aaaagttttg
    17701 aaatgatcgc attgcaacag ccagttacaa ctgatttgcg tatgatcatt acggtcatga
    17761 aagctagctc ggatttagaa cgtatggccg atcatgcggt gtctgttgct aaatccacga
    17821 ttcgcgtaaa agggcagacg agaataccgg aaatcgaaaa agagatctcc gatatgtccg
    17881 attatgtgaa aaaaatggtg gacaacgtcc tagttgctta tgtcaaaaca gacgaaaaag
    17941 acgcacgtac gatcgccaat atggatcaaa gagtcaatga ctatttcaat cgaatctata
    18001 atgcaacaat caaaaatatg caggaaaatc ctgagacagt catcagtgga acagactact
    18061 tgaatgttgc cagttatcta gaacggatcg gagactatgt gacaaacatc tgtgaatgga
    18121 tcgtctactt ggctacaggg aaaatcactg agttaaatac gaaccacaat gagattttct
    18181 aaactgaagc tgtgattatg ccgtttgttt atgaaaagag gttgtgaaaa agctgttcta
    18241 catcaagtaa taagaacagt caaacaaaaa atagctcctc atatttttcg tttggctgga
    18301 cttattaccg cagatgcaag cttttgaaca ccgtttattc gggaaagggt ttgtgacaaa
    18361 acatgtcaca aacccttttc atcatttcat agaaaggata aagaatgcaa aaaaaaatta
    18421 gcatcatcac tatatttttt atttgtgtgc ttacttcatg ggttattcag catcaagccg
    18481 ctttatatga agtacctgtc atcgagatag aaaaagtacg aataaaagag gatcatcaat
    18541 tgattacagg aaaactgatg aactcggatc aaaaaggaga atcgatttat ttatcggttc
    18601 cttatcaaaa aaatcaaata gaaacagcaa atataaaagc gagacaaatg tatctagtcc
    18661 aaatgcatgg agatgaatgg cagctagtag caaaaaaaaa tgatggctgg cttttcttct
    18721 tcacaagtat cttcattggc gccatgttgc ttattggcgg aaaacagggt ctatttgctt
    18781 taggaagtct catcatcaat atcgttttac ttttactttt gttgctcatc tttactaaaa
    18841 cagaagggat ttctctatta aataaatcta tcctgttcgt cgttataggg atgattatta
    18901 gcatattggc tttagatgga tggaatagaa gcagtataat caaaatttca gctgcagtta
    18961 cgagtgtttt tttagcattt tttatttgtc ttttgacaat gaatcattgg aaagaccaag
    19021 gactgcgcta tgaggaactg aattatctga cacgaccgta tcgttctgtt tttctttcta
    19081 gcgtactgat tggaacagct ggaggaacac tcgataccgt tatcacagtc attgcgacat
    19141 tagaagaatt gactaagcga caaccagaaa taacagcgaa acagttatgg ctttcaggaa
    19201 ggcaagtggg tcgagatgtg attggctcga tgaccaatat ccttttgttc gtctatatca
    19261 gtgggtcagt tccatcgctt cttttatatt taggaaatca atggtctttc aaagaaacga
    19321 tagaacagca tttatcattg gaatttttac gcgtcattgc tggcagctta ggaatagtat
    19381 tatcgatccc catctcggtc ctcttttttc tgactgtaag gaggctgaaa cgatgaatgt
    19441 acttattata cttgttggga ttttagcaat cagcgtgcta cttattggcg gtacgcaagg
    19501 tatgtatatt cttttaggct tattcatcaa tttagggatc tttttcctgt tgttattcgg
    19561 ttatcatcag aagtggccga tactggcatt gtcggtcata ggatttttat tgattgctgt
    19621 ggttatctta ttttttatca acggttataa tctaaaaatg cgtgcagctt ttgtgagtat
    19681 actgatcttt ttattttgtt tcctactatt gcttcctatc actgattttt tagcgatcca
    19741 aggttttaca tcggtagaac tagaagaatt atctggctta gataagactt tagcgattga
    19801 ttttagatta cttacgagat cgttgcttct gattagtctt agtggtgctg ttttagatgc
    19861 gagtgtagca atttctagtg ggacattcga agtatatcag gcaaatccgc aattatcttt
    19921 ttaccaattg cgccattcta gtttcgctat tgctaaaaaa atattggcgt caacagtaat
    19981 gacacttctt tttgcattca tgggaagttc tttagcgttg atcctctggt ttattgattt
    20041 agacattcct tttcagcaag tgatcaatga aaaatcattt gttctagaat atacgatggc
    20101 tattcttacc acattgtccg cactaattgt tcttccttta acctgcgtga ctacagcata
    20161 tctctttaca aaaaagaaag aacaactcaa gatggagtaa aagttacaac ttaagtccta
    20221 tgacattttt actgaatcag tgcataatat ggatgtaagg aaaagcttgc aaacaatttt
    20281 gcttaaggag gcaatttcca tgaaagaaag agaacgtatt ttagatttag tgaaaaaagg
    20341 aatcctttcg actgaagaag gcttagattt attagaaagc atggcaactg aaaaagacga
    20401 aaagcaaatc aaaaaagaag cagacaaggt aactgctagt cataaagaaa aagataaggc
    20461 aagtcaattg atcgataaac ttgaaaacgg cgaagaagaa atttctgagc cagttgatcc
    20521 aaaggaaaga gaaagacaag atcaagaaaa cttagaaaag atcttagatg aactagcaac
    20581 tgaagcgaat aaaacttcgg cgcgtctaga tgaagtaaat gctcaattcg ctgacaataa
    20641 ggcagcacgc aatgagaaac aggaagcatt gatgcagctg aatacaaaag aagaattagg
    20701 cgagttgaca gaagaagaat tagcacagcg tcaggcgtta gaggcagaaa tcaaagagtt
    20761 agaagctagt ggcgatacac tgttagaaga acaattgaaa ttagaagcag aattaaaaga
    20821 tatccgtaag aatcaatggt ctgagaaaaa agaaacgttt actgataagt ttgaattgcc
    20881 agatgattgg aaagatcaag caacagatac actgaatcaa gtaggcgaaa aaatgtcaga
    20941 agctggatca cagttaggca aattcttgaa gaaaacattc caaactgttt cagaaacagt
    21001 gaatgacaac atggaatgga aagatgtcag cttacgtgtc cctgggattg cgacaacgaa
    21061 atttgagcat gaattttact atgaagctcc tgcagcaagt atccttgata tcaaggcagc
    21121 aaacggaaac gtgacgttaa aaacgtggga cagcaacgat gtgaaagttg aagctaagat
    21181 caaactttac ggaaaaatgg gtgctgaacc atttgaagca ttctctgaac ggagccagat
    21241 tgaggttaat gaagatcata tctccttcca aattccaaat aaacgtgtgc gtgcagacct
    21301 tgttttctac ttacctaaac gagtatacga ccacgcagct attaaacttc taaatggcaa
    21361 catcatgatt gaaacattgg aagctaaaga tatttataca aaatctacga atggtaacat
    21421 catcgtggac caattaactg caacgatgct ggaagtagaa ggtgtaaacg gcaatatcga
    21481 tattcgaaac gggaacatct tagattctat tatcgaaaca gtaaatggta cagtgacatt
    21541 tggtgcgaca gcagaaaatc tttctgtgtc tttagtaaat ggtgatgtac gtcttacgat
    21601 taaagaagat aatttgaaaa aagtagaagc aagttcagtg aacggaaatg tcaaagtcgc
    21661 tttaccagat ggcatcggtt tggaaggaca tgctaaaaca agtcttggaa gtatcaatag
    21721 ccgtctgtcc aattacgaag tagttcgtga gaaaaaagaa cggacaaacc aaatgttgca
    21781 attccgtcgt gtttcagatg atgagatcgc gcaagtgcaa ctttcaacga caacaggaag
    21841 tatctattta aaagatacag ataaataaag aggttgtgaa agagatgttc agccttgagc
    21901 aataagaagc gattttgtaa aatagcttcc cctattttac aaaattgtgc cttattgccg
    21961 atagtctatc tcttgaacac cgtttaccaa gagattatga acctgccaat tatcgccgag
    22021 tcacaaggaa taatttcaga aaaagctcgt cacgttttat gaatatggta atttgtgacc
    22081 gagaattttg cagaaagaca ccgattaaga agaaggttgt cacaacgatt ttcttcattt
    22141 aaaatagtag aacagaggtg agacatgatg aaaaaaaagt tgacaaaatc taataaaaat
    22201 gttgttttga cagggacttt agcaggaata ggagaataca tcgggattga tccgactgtt
    22261 atccgtgtgc tttatgtatt cctgagctta gttgcttttg gaagtccaat tattttatac
    22321 attttattag ctttgattat cccatctgcg cgttctgcac gaacaaacta tggacataac
    22381 aatgcatatt atgaaaaaaa caattatcgt gaacaaactc gtgaacaaac aaaaaaacgc
    22441 aaagaagcgg aaaaaatcga tgatgacgaa tggagtgact tttaatgtcg tactttcaac
    22501 gtattttagt taatacgtta acattcatat cattggctgt tctttttcct gcgatggtcc
    22561 atgtgagcag tatttggacg gccgttcttg ccagcttcgt attgtctgtg ttgaatatgt
    22621 ttgtcaaacc gattttgacg atactgtccc tgccctttac attagtcaca ttgggattgt
    22681 ttacgtttgt tatcaatgca gcgattctta aaatgacctc cttttttgtg ggagcagaac
    22741 attttagttt ttcttcattc ggtgcagcgt tgttagtagc tatcttgatg tcgcttgtca
    22801 atgtaattgt cagcgaacat aacagtagaa aataaaagaa agctgtgccg aactatcggc
    22861 gcagtttttt tatttagaat caggaatgcg ttttctatta cattggtaat taaataaaaa
    22921 cgtttttttt aattggtagt acttttggct agacaatgat aaaatgatag agaacactct
    22981 aatagaaatg aggtagcatg aatgtcagaa gttgtaaaag ttagccaatt agtgaaaaaa
    23041 ttatctttgg aaatcgtgac aggcgacgag aaaagcttag accgtgtcat cgtaactggg
    23101 gacatctcac gcccaggact agagttgact ggttatttta attattattc ccataatcgt
    23161 ttacagctat ttggcagtaa agaaatcagt tttgcggaac ggatgatccc agaagagcgt
    23221 ttgatcatca tgagaagatt atgtagtgaa gatatgcccg catttattat ttcaagagga
    23281 ttaccagctc cagaagaaat gatccaggca gcaaatgaaa atgggatagc tgtactgcgt
    23341 tctcccatct caacttctcg tttattagga gaattatcca gttatttaga tggtcgcctt
    23401 gcccctcgaa caagtgtcca cggtgtttta gtggatgttt acggattagg tgtactgatt
    23461 caaggtgaca gtgggatagg aaaaagtgaa acagcgttgg agttgatcaa acgcggccat
    23521 cgattgatcg cagacgatcg agtagatgtt tatcagcaag atgaattaac ggtgatcgga
    23581 gaaccgccta aaatcctgga acatctgatt gaaatccgag gaatcgggat cattgatgta
    23641 atgaatcttt ttggtgcgag tgcggtacgt ggctctatgc aagtccagtt ggctgtttat
    23701 ctggaagcat gggcaaaaga taagaaatac gaccgtctgg gctctgaaga cacgaccgtt
    23761 gaaatcgcgg aagtgggcat tccacaaata aaaatccctg tcaaaacagg acgaaacgtt
    23821 gcaatcatca ttgaagtagc cgcgatgaat ttccgtgcac gtacaatggg ctttgacgca
    23881 acgaaaacat ttgaagaaag attaagtcgc ttgattgaag aaaactcagg caacgactag
    23941 caaggagtga caatatgtta gcacaaatca atcgagttgc ttttgagctt gtagggattc
    24001 ctatctactg gtatgcattg atcatcgtat caggtatcat tatcgctatg tggcttagca
    24061 gcagagaggc tgttcgggta ggattaaaag cagatgatgt gacagatttt atgttgtggg
    24121 gattgcctat ctccattatt ggcgctcgct tatattatgt tttatttgat cttccccagt
    24181 atatagctga tccaatccaa atattcaata tccgttcagg aggcttagct atttatggcg
    24241 gactgattgc aggcgcgatc acgctgtatt tttttactaa atatcatttt atttctttgt
    24301 ggactttttt agatatcgct gcaccaagtg tattattagc acaggcaatc ggtcgttggg
    24361 gaaatttcat gaaccacgaa gcatatggac cagacacgac gcgaagcttc ttagaaagtc
    24421 tgcatttacc gcaattcatc attgataata tgtatatcga agggatctat cgtcaaccaa
    24481 catttcttta tgaatcactt tggagtttga ttggatttat tttgcttttg tttttacgaa
    24541 agaaaccaca ccttttgaaa aaaggcgaag tccttttatg ttatctaatg tggtatagtt
    24601 tcgggcgttt ctttattgaa ggattacgta cagacagtct ttacttttta ggtgtgatcc
    24661 gtatttctca attattatct gctgtgttat ttgtcggtac attgattcta tttatttggc
    24721 gcagaaaaaa aggaaattta ccggattatg accgctcatt aggaaaaaat caacaaatta
    24781 tctgattttt agagcgttaa aaatgaaaaa aatttcagga gtggtaaaat gaattataga
    24841 gtagcggttt taggaccagg ctcttggggt actgctttgg cacaagtcct cgccgaaaac
    24901 ggacatgacg ttcggatttg gggacatcgt gcttcacaag tagaagaaat caacacccaa
    24961 cacacgaatc aacgttattt accagatcat tatctacccg attccatcca agcattcacc
    25021 aatatggaag atgcagtaaa agacgcagat gcggtgctat tcgtcattcc aacgaaagca
    25081 atacgttctg ttgcaaaaga actagtgcct aaattgaata caaagccagt gatcatccat
    25141 gcaagtaaag gattagaaca gggaacacat aaaaggatct ctgacatctt gttagaggaa
    25201 atcccagcag aaaaaagaaa agctgttgtt gttctttctg gaccaagcca tgcagaagaa
    25261 gtagcaaaac atgacatcac tacgatcaca gctgcaagta cagatgcaaa agcagcaaaa
    25321 tatgtacaag agctgttcat gaataattat tttcggatct ataccaatcc tgatgtgatc
    25381 ggtgtagaga tgggggcagc tttgaaaaat attattgcta ttggtgctgg cgctattcac
    25441 ggcttagggt ttggcgataa tgcaaaggca gcaatcatga cgcgcggatt agctgagatc
    25501 agccgtttag gtgtagcaat gggtgccaac ccgctgacct ttatcggatt aagcggaata
    25561 ggcgatctag tcgttacttg cacaagtgtt cattctagga actggcgcgc aggcaacttg
    25621 ttaggacaag ggcagcccct agaagaggtg ctagccaata tgggaatggt cgttgaaggt
    25681 gtgaatacta cccaagcagc gatggaacta gcccaaatga tggaaatcga aatgcccatc
    25741 actcaaacaa tctatgaagt gctgtatgaa caaaaagaaa tcaaaacagc cgcaaaagaa
    25801 atcatgttac gtgatggaaa aaaagaaaat gaattctcat taacaatttt ttgaaggagc
    25861 gaatgtatat gaaagttaaa aaagcagtta ttccagcagc aggattagga acaagatttt
    25921 taccagcaac aaaagctatg gcaaaggaaa tgttgccaat tgttgataaa ccaacaatcc
    25981 agttcatcgt tgaagaagcg ttgaaatcag gtatcgaaga catcttgatc gttacaggaa
    26041 aagctaaacg accaatcgaa gatcactttg attcaaatat tgaattagaa acaaacctaa
    26101 gtgaaaaagg aaagaccgaa ttattgaaat tagtagaaga aacgacagat gtcaacttgc
    26161 atttcatccg tcaatctcat ccaaagggtt taggacatgc cgttcttcaa gcaagagcct
    26221 ttattggaaa tgaaccattt gtcgttatgc taggcgatga tataatggaa gacgaagtac
    26281 cattgacaca gcaattgatc gatgactatg agcgtacaca cgcttctacg attgccgtta
    26341 tgcaagttcc tcacgatgaa acttcaaaat acggaatcat caacccaggg gaagtactgg
    26401 aagatgggtt atacaatgtc aaaaactttg ttgaaaaacc agatccaagc aaagcaccaa
    26461 gtgatttagc tattattggg cgttacttat tgactccaga aatctttgat gtattatcag
    26521 aacaacaacc aggtgctgga aatgagatcc aactaactga cgcaatcgat acgttgaata
    26581 aaacacaacg cgtatttgcc cgtaaattca caggtaaacg ttatgacgtt ggtgacaaat
    26641 ttggttttat gaaaacaagt attgaatatg gtctgacaca tccagaagta ggggcacctt
    26701 tgcgtgaata catcatgtct ttaggtaaaa cattagaagc agaaggaagc aagatcaatc
    26761 ataatccaaa aggaataaca gaaatcaaag aagaaaagaa aaaataatct ttttttctaa
    26821 cgagcttcag ctgctaattg aataaaaagt agttgaagct cgtttttatt tgtttaaaaa
    26881 acacgaaaat gctatcctta tagaaaagag agacaagaag ggagtttgat catgtcagga
    26941 ggagaaattg caggattgat cgcagcaatc gcctttgcga tacttgtcgt tttcatcgtt
    27001 cgcgtattgt tgcaagtttc caaaacttta gaaaaagtag accagactgt cgcagaagca
    27061 aatacgacaa ttgagatcgt cacacaagac gtagatcttt tatctagaca agtagaaggc
    27121 ttgctagtca aaagtaatga gctgctcttg gatatcaatg gaaaagtcgc aacgatcgat
    27181 ccattgttca cagcagttgc tgatttaagt gaaagtgtat cggaacttaa tcattctagt
    27241 aaaaacatcg tgacgaaagt cggcggaatc ggaaaaacga cagctaaagc gactgttgcc
    27301 ggaaaagtcg gtggaacagc gatgaaattt tttaaaaaca aaaaacatac tgaaacgaca
    27361 ggaggaaaat aagaatggct aaaaaaggtg gatttttatt aggtgcagta gttggaggta
    27421 cagccgcagc aatcgccgca ttattgttag cacccaaatc tggtaaagaa cttcgggatg
    27481 aattggctgc tcaagcagat gatttgatgg acatggcttc agattacaca gatatggcaa
    27541 aagaaaaatc atctgagtta acaggtattg caaaagaaaa agcgcaaggt ctttctcaac
    27601 aagcagggga tctagcaggc aatgttaagc agaaggtaag tgaatcagca gaggaagcag
    27661 gaagtgaatc agatgatatc ttgttaaacc tcaaagaaca atcagctgat ttatctaatc
    27721 gtttcaaaaa aacagcagaa gatctgaaaa agccagcaga cgaattaggt agtattgcta
    27781 aagatacttc agaagatatc atgatcgatg taaaagatgc tgcagataaa gcaggagaga
    27841 cggtcaatga aggaatgtca gaagcaaaac cagttgcaga tgacacaaaa gaaatgttga
    27901 aaatggcaaa agaagatgca aaagaagcta aaaaagaaat gaaagataca atgacagatt
    27961 cgactaccaa tcactaagat tactcatttc tagaataaaa aaggtgagag cggaccagct
    28021 ctcacctttt ttattttcat tactctaaaa caagtaattc ttttggtgta gtcgtgaatg
    28081 gcacacaacc atcacttgtc acatacacac aatcttcgat acgtacccct actttttcag
    28141 gaatatagat tcctggttca agagagaagc acatcccttc ttcaatcact aagtcgtttc
    28201 cttctacaag agaagggaat tcgtggacgg ttgttccgat tccgtgacct aaacggtgat
    28261 tgaaatattc gccgtagcca tagctgttga tcacgtttcg tgcgatttga tcaagttcac
    28321 ctgctgtcac acctgggcga actgcctctt gtgcggcaag ctgtgcttca agagtgatgt
    28381 tatagatttt ttcttggaac tcagttggtt tttgataagc aactgtacga gtagcatcac
    28441 tgcaatagcc attccaaata acgccaagat cgaataacac tagttcattt ggtgaaacct
    28501 tcgtgcttcc tggtgtacca tgagggcttg cagcatttgc tcctgcaagg actaatgtgt
    28561 cgaaagacat ttgtgaaaca ccttgttttt ttaattggta ttcgatttct gcgataattg
    28621 cttgttcagt tacgccttct tttactgttt tgaagccaat ttcaaaggca acatcggccc
    28681 atttaccagc ttcgatcagt ttgtcttttt cttctaccgt tttgatcagt tgcagacgct
    28741 ggataataga agttagatcg gtgtaagaat ctgtacctgt caagatatca gagatagcat
    28801 cgaagcgatc gacagtcaga gcattctttt caatacctac attaccaggt tgcccatagc
    28861 gtgcgcgaag ctcttttccg atgatctccc aaggattttc gctgtctaag tagccacgta
    28921 catcgtacgc ccagctgctg ttttgagcat cttcaacttc cagagcagga gtgaaaagga
    28981 aaggatcttg ttccagaggg ataaaaagag ctagtacacg ctcgtgagga tcactttcat
    29041 atccggaaaa gtaagcgatg tggccagggt cgctgatata agccaattct acttggtgtt
    29101 gtgccatcca ttgacgtagt tcattgattt ttacgttatt catttggcaa cctactttct
    29161 aaattaattt tgtattcaat aattgtacca ctgattgatt aaaagaccaa cgttcttaaa
    29221 actgaaaaaa gtaaaaattt tcatgaaaac agagataaaa cggttgcaat tgttgagatt
    29281 atttgatatc ctattttaga aatgtaagac gtattttcag aaaacatata aaatatgaat
    29341 aggagaacac aaatggaaaa gcaaacaatc actatctatg atgtagcgag agaggcaaat
    29401 gtttcgatgg ctactgtttc acgtgtcgta aatggcaatc caaacgtcaa accagcaaca
    29461 agaaaaaaag tcttggaagt tatcgatcgc ttggattatc gtccaaatgc agtcgcacgt
    29521 ggcttggcaa gtaagaaaac aacaacagtt ggggtaatca ttcctgatgt cagcaatatg
    29581 ttctttgcct ctttagcacg tggtatcgac gatgtggcaa caatgtacaa atacaatatc
    29641 atcttagcaa actcagatgg caacgatcaa aaagaagtca atgtattgaa taatttattg
    29701 gctaagcaag tggacggcgt gatctttatg ggtcaccata tcacagacga aatccgtgga
    29761 gaattttcac gttcaaaaac gcctgttgta ctagctggtt ccattgatcc agatgaacaa
    29821 gtaggtagcg taaacattga ttatgtgtca gcaacaaaag atgcagtaaa taaattagca
    29881 aaaagcggca ataaaaaaat cgcttttgtc agcggtgcgc tgattgatcc tatcaatggt
    29941 caaaaccgtc taaaaggcta taaagaagca ttaagcgaaa acaatctacc atatagcgaa
    30001 ggattgattt ttgaggcaca atacaacttt aaagatggtc tgtctctagt tgaccggatc
    30061 cataatagcg gtgcaacagc tgtctatgtt tcagacgatg aattagccat tggtatcttg
    30121 gatggtttat tagaccgcgg cgtaaaagta ccagaagaat tcgaaatcat cacaagcaac
    30181 aattctttac tgacagaagt agctcgtcct cgtttgacaa gtatcacaca accattatac
    30241 gatttaggag cggtatccat gcgcctattg acgaaaatga tgaataaaga agaagtcgaa
    30301 gaaaaaacga ttatcttgcc ttatggaatc gaagaaaaag gatcaactaa ataataaaag
    30361 atcaatcatt taaaggttgt tccaaaagtg gtcaacttct ggaacaacgt ttatacgaga
    30421 aagggggtta tgacaaaatt ttgtcataac cccttttttt ttgtgacttt aaaaaaagag
    30481 aaaatggcgg aaacaaacct tacgtccatc gtatacgaca caatctgtgc gtagatgcta
    30541 cattttg
//
LOCUS       DACGLE010000027        29459 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0534
            SAMEA3304449-rid9560453.denovo.028, whole genome shotgun sequence.
ACCESSION   DACGLE010000027 DACGLE010000000
VERSION     DACGLE010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304449
            Sequence Read Archive: ERR985064
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 29459)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29459)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702225.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702225.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 20:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,045
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 27 of 67
            Pseudo Genes (incomplete)         :: 25 of 67
            Pseudo Genes (internal stop)      :: 19 of 67
            Pseudo Genes (multiple problems)  :: 4 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29459
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304449-rid9560453.denovo.028"
                     /strain="VREN0534"
                     /db_xref="taxon:1352"
     gene            complement(159..1469)
                     /locus_tag="IXT20_001349"
     CDS             complement(159..1469)
                     /locus_tag="IXT20_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HAQ4859804.1"
                     /translation="MRTKKGIINALNFKTNDTQQDDTIILQKAIDQGAKEHLPVFIPK
                     GIYLVGALFLKDKSHLIFEEGAVLKGRTDIEAFPEIDTRVAGVEMKWPAAILNVLSAK
                     DILIEGKGIIDGQGDHWWELYWGKDQKSGTRAEYDQKGLRWIADYAIKRPRACLLYHA
                     EHVVVRDLIFQKSGFWNLQITYSNDVLVEKVIIRNNDGPSTDGIDIDSSTNVRVYECD
                     LACGDDCIAIKSGRDGNGARVNRKSSRIEVARCKIRSGYGVTIGSEVSAGVSDVYIHD
                     IDFFQSDCGIRMKSSKERGGVIENIRVENLNMIDVQFPFSWIMDWHNEYNRKNSNDLA
                     QMPESWQAVAEEIPESKQMTKVKDIHIKNVQACLSPGYSLPSRAFDLVAFPEKPIEDV
                     CFSHCQITAKEFGRIEAVQNLCFKSCDLSIETGNTAANNTFDNR"
     gene            complement(1481..2899)
                     /locus_tag="IXT20_001350"
     CDS             complement(1481..2899)
                     /locus_tag="IXT20_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ4859805.1"
                     /translation="MLSQNAVTANTIKKEKKEFGISDKIGYMFGDLGNCFILGLVNSF
                     LMIYYTNVLGVSGAIVGTLFLISRILDAFADVMVGRLCDVSELTETGRFIPWIRKMKY
                     PFCLVTVILFLPFVQHLPMTVKIAYIFVSYIVYGIFLSTINIPYGAMAAAISSNPNDR
                     TSLSTFRSIGSAIGGSTTGFFIPILMYTHNKSGQQVVSGNHFFWISIGCAAIAFVAYM
                     LTCKLTTERVRVVKNEKVSASQLVKGLLANRALIILVIVDIFIVINQILAGTNMTYLF
                     NDYFHNKQAMSVALLFTYGTVVVLAPFATKLTKLFGKKEASVFALLASSCMYLLMYFL
                     HITNAWVYLILLFIATLGSGLFNLMVWAFITDVIDYHQYKTGLREDGTVYGVNSFARK
                     VGQACAGAVGGFMLALIGYQSSTTGGAIQSVLVQERIYTMANLLPAFCLFVSAIILLV
                     GYPLNRSKTVFIGEELKKINER"
     gene            complement(3139..4707)
                     /locus_tag="IXT20_001351"
     CDS             complement(3139..4707)
                     /locus_tag="IXT20_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HAQ4859806.1"
                     /translation="MQTIKVKNPILRGFNPDPNILRVKERYYIIVSSFEWLPGIRVYE
                     SKNLVNWSHTTDILTTQVSLQGNPTNGSIWAPQISYADGLFYLVYTDVKSAGRPFKDV
                     HNYLITASEITGPWSEPIYLNSNGFDPSLFHDLETGKKWLVNELWDYRLDTPNKSSGI
                     VLQEYDAEKKKLDGPIYKIFAGTELAKTEAPHLYFRNGYYYLMTAEGGTGRGHSVTVC
                     RAKNITGPYELDPGFPMLTASDKPASTLQCTGHGSLVQTPSGRWYMTYLCTRPLNGAA
                     ILGRETAIQEVYWTSDDWLRLKDSQTTPMEEIMIETNEEVQQEKDHQFMDTFEGELKK
                     NWNARRILPNADWCDTKCRPGFLRLISGESLQSTFEHHLLAIRQTDFTFDAETAFEYM
                     PNNFNQMAGLVLYLNESNYIYCYVTWNEDKGKCLRMIESENGVAQIEDWTIPLTEKKT
                     FLKVTVKKETAQFHLKDVSTEEWKKVGPQKNMCILSGGFTGNFIGIAVHDLNKKRGSY
                     ADFEFFKYDGKDHL"
     gene            complement(4711..5121)
                     /locus_tag="IXT20_001352"
     CDS             complement(4711..5121)
                     /locus_tag="IXT20_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HAQ4859807.1"
                     /translation="MKRKWELLLGMVGGSLSLIFFGGLAVTLSNMSASEFKKSYQSLA
                     VDHSTLSLENTFGLLQDMTGLFAVVLFISLAFLAVALFLTAKGKYLTTATGLYFITGF
                     ILLIGTQFIAFPFAFFYFAAGAFSLYRVRMRKGA"
     gene            complement(5118..6041)
                     /locus_tag="IXT20_001353"
     CDS             complement(5118..6041)
                     /locus_tag="IXT20_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pectin esterase"
                     /protein_id="HAQ4859808.1"
                     /translation="MRMKDKLIIGKNAIDTPTDFETIQEGIDYLAGLPDSHPKVLIVK
                     EGRYDEYIESRLSNFKLVGAGTVVISGNRFAKQEYLDGSQLGTFRSATFFLEGSNVVL
                     ENLHIENTAGPGEKVGQAVALFNSGDRVSLKNCRLSGYQDTLCTGPLPDLQKDGTPFC
                     TPASANPEYCRQTYEYCWIEGTVDFIFGGADAVFHQCHIHSLASKNPGYITAASTPKN
                     QKTGYRFESCTLTAEPDTRNVYLGRPWRPYANVLFEACKIGGHVHPTGWDNWDDKKNE
                     QTVKFSERNNNYETSIKRPEWIHLEKQGVQK"
     gene            complement(6038..6916)
                     /locus_tag="IXT20_001354"
     CDS             complement(6038..6916)
                     /locus_tag="IXT20_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HAQ4859809.1"
                     /translation="MQLNDFLGAPSSRHTPSGKRLYREKQNGFMLEYYQLYLNELETV
                     PALYAYPEKEGPFPPVLYLHSHGGDFSVGKSELIHGAPYLASPSFAEVLTGMGYAVWS
                     IDAWGFEERGGISESELFKQFLLTGKTLWGMRIYDVISLIDYLETREDTDTQRLATIG
                     LSMGGLLSWWVTALDSRVKVCIDLAAQVDIEILLLHRRLDHHGFYYYVPNLLTAYTTL
                     AIQEKIAPRPRLSLVGKNDTMCLDEGVLKLRKGLDKKYQSMGSPENFSSFSLTGGHME
                     TQEMRNIWKQFIREHL"
     gene            complement(6903..8240)
                     /locus_tag="IXT20_001355"
     CDS             complement(6903..8240)
                     /locus_tag="IXT20_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HAQ4859810.1"
                     /translation="MYDILTFGASIDELNTEAIQQAIDAAASDGGGTVVVPAGEFLTG
                     ALFLKSNVELHLSAGAVLKFSDDPKDYPVVHSRWEGVHRKVYASCIYAQNVENISVTG
                     FGTLDGNGKKWWHTFRNEPDNLAYPRPKLMSFHNCHRITVKDIKLIQSPSWTINPILC
                     SNATFDNLTILNPADSPNTDGIDPESCKNVRISNCHIDVGDDCIAIKAGTEDTYERIA
                     CENITITNCTMVHGHGGVVLGSEMSGSIRNITISNCIFQETDRGIRLKSRRGRGGIVE
                     DIRVSNIVMDNVMCPFILNLYYFCGPRGKEPYVWEKKAYPIDERTPAFRRIHFSNITA
                     RNVHASAGFIYGLAEQFIQEITFDNIDVSMAQDAVPGVPAMMTGIEEMSRQGFYIGFA
                     GKVSFNQMSIENHEGPAFLLENTKEISIEKFQSNQSENMDELVVRKNFAYAVE"
     gene            complement(8243..9346)
                     /locus_tag="IXT20_001356"
     CDS             complement(8243..9346)
                     /locus_tag="IXT20_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 88"
                     /protein_id="HAQ4859811.1"
                     /translation="MENWSKKLAKSIMERTPRLYEEKWYKGKWSYDYGVVLKGFQLLW
                     ERTQEKIYFDFIKDNIDYFVQEDGTIRGYSVEEYNIDHVNTGKLFFLLYKETGEEKYK
                     KAAELLSQQLANHPRTSEGAFWHKEIYPYQIWLDGLYMGSPFYAEFIQNFSEEKDYQD
                     VLRQFEISYKHLFDPKTNLLIHAWDEKKVQPWADPKTGLSHHFWSRSIGWYLMAAADT
                     YELLHEETDCSLLKEILEKTLAALLPYQEENSGTWYQVTTETKRKGNYLEASGSSMFV
                     YAMAKGIRLGLLNKEEWLPQVKKSHQGLLDEFVLETKEGWLNLNKNCQVAGLGGADQR
                     DGSYAYYISEPIICNDQKGVGAFLQALIEVEAL"
     gene            complement(9420..10928)
                     /locus_tag="IXT20_001357"
     CDS             complement(9420..10928)
                     /locus_tag="IXT20_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAQ4859812.1"
                     /translation="MKKATLVKLGLSFAGILALAACGSSSSNSSSETEDGKAKITWLN
                     ILHHASPPTDTIVNLIEEKTDTELTFNWIPDASKEERLTTALASDELGDIVTLQIAML
                     KNSSVRNALKSGVFWDVSEYLKDYKNLSQISEDRIEAASIDGALYGVPIQKDYARAGL
                     VIRKDWLDNLGLDVPTTMDELYEVAKQFTENDPDGNGVDDTVGFGDRAPSDIRYTSFK
                     LFTSYFGAPNGWAVDDNGKFTPEFDTEEYMKALNFSRDLFENGYLAQDFAVTQKTDQQ
                     EQFAQGRTGIYTGMVDIKNLKTMGQGIQDDMELVPVNKISDGEGGGYHVWSEGNGIGG
                     LLAFPKSQVKDEAQLKKLLQFVDDLLEEDVYKLMTGGIEGTHYELNDDGSIEFIDMDL
                     WQQEVQPLSSSRPSEVTFSFKDTDPEKEFSNQLIKENEEFAVMDPSVPLDSPTNNEVG
                     SEIEKIVIDATVQYIMGQLDEDGFKKAVEDWKAQGGDRITQEYEEAYKAAAQ"
     gene            complement(10894..11586)
                     /locus_tag="IXT20_001358"
     CDS             complement(10894..11586)
                     /locus_tag="IXT20_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HAQ4859813.1"
                     /translation="MLQSLDKINGRLNRILQLVYLNFLWGGLVIAGLGVFTFGPASYA
                     MVAVIRKWLRSKETFPLTKTYFTYFKENYRETLLMSWIYGAIGIVLLVDLSFIHNWYL
                     RIALYIAAFIYVLSALYIFPIMAHYDWKGYFFKMKMAFLFGFSHLHYSLVLILSMVGI
                     YLMIIQTMPGVLTFIGGSLFFSAVTWTANSIFCHLEWKNSYTEKTEENKNIIKTVKEK
                     IYEKSNARKIRS"
     gene            complement(11608..12582)
                     /locus_tag="IXT20_001359"
     CDS             complement(11608..12582)
                     /locus_tag="IXT20_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ4859814.1"
                     /translation="MESVKKQEPFQLTLSKKEEKLRRRKKIIAGIKTNKFLYVMILPG
                     VLYFLIFRYLPMGGLVIAFQDYQPFLGIMGSPWVGFKHFIRLFTEPTFFTLLRNTLIL
                     FGLNIFVFFPMPIILSLLLNEVTNKRLKNGIQTIIYIPHFMSWVIIVSITYVFLNVDG
                     GVLNEFLATMGFEKISFLTSPEWTRTVYIGQVIWKELGWSTIIYLSAITAVDTQLYEA
                     AEMDGAGRLRKTWHVTLPAIRPVIITLLILKIGQTLDLGFEHMYLLLNSLNRSVAEIF
                     DTYIYTAGLKNGQLSFSTAMGLFKGVVGLFMVILANKIAKKLGEDGVY"
     gene            12882..13754
                     /locus_tag="IXT20_001360"
     CDS             12882..13754
                     /locus_tag="IXT20_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAQ4859815.1"
                     /translation="MKKKVSIGERSFTLFNNTFLILLALICIVPFLNIIATSFASTQE
                     VVAKKFILFPTTFSLDAYRYILSTPTIFRALAVSIGVTGVGTIVSMCATSLMAYGLSR
                     KYLFGRGFVNFLVVFSMLFSGGMIPTFLVVRSLGLVNSYWAMILPVAVNAMNMIIMRN
                     FFQALPDSLEESAKMDGCTDFGVFFKIMLPLALPSIATISLFYAVTYWNTYMTAILYI
                     NDSSKWPIQILLRQIVIVSSGMQAESSAVDVIPPAQTVKMAVIVIATVPMLIVYPFVQ
                     KYFVKGALVGSVKG"
     gene            complement(14212..16410)
                     /locus_tag="IXT20_001361"
     CDS             complement(14212..16410)
                     /locus_tag="IXT20_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ4859816.1"
                     /translation="MKKSTVFYKIFLPMFLLGAVLVIGFSLFIYQNTYESIESSYLTD
                     KKNLLRQVKTNVEWKIRTIEYSFSTYGSTKNFSDIFKHPLTYTDYSTYSEIRKELNFI
                     ETIVMDDNNYDLVSLAGKWGVINGSLSQLTEEEIEKYQQKYINNKNNLFWQKKNKGIE
                     MVISLPMFEKEKYALGIATIEKHTIEQVVGESGEDILSIENEVGNLFTTQTVKNKEQP
                     TVLTDVDLDYNQLKVIREKDKTYIVLKSDYNNWIYRLEVDSSAIGAMIRNLRIGLVAV
                     SLTLVGLIGLLSYLFSDRFARPISQIQERLNLKSKGFSGKELDLVAQSVSRIIGEKEA
                     LSAHLVTQKPQLETLFVLSLFRNRVERRELDQRLQQFGYSSQNDCYYTALVQIDLLED
                     SHGGERDLLLLAINNMITEIVPKENRMLPIVLNEEMQATIYRMDKDDAEASKKVMEYC
                     RLIQKRVKEYLNLTISIGISNRFETLNESKQSVDRAKEALYYRVNTGPSSIIFYQEIV
                     PAAHEKTLIRYPIEEQNRLFEAIRSGNQEVKNLVHELIDALFAQNKNPLSREVVSVRL
                     INELVQLGQLLGVDSKNFDNMKQIYVKALNNYHPKELEKLVLEQLVLPITECSQAITD
                     HEFKNLSENIMHIVYTEYDRDLSLDVIADRLHYNPNYLSNVFKKETGEKFGDFVQNHR
                     LEVAKKWLKETNLSVKEIAERLQYRNPQNFIRFFKKKESITPGEYRKKYL"
     gene            16642..17634
                     /locus_tag="IXT20_001362"
     CDS             16642..17634
                     /locus_tag="IXT20_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAQ4859817.1"
                     /translation="MVTIKDVAKAAGVSHTTVSRALNNSSLIKNETKQKIRQLAKEMN
                     YSPNYSAKGLVNQKKYLIGLFFSSLHKGTSSNFLADSIAGIHSVLDTNYSLSVESIDV
                     VDLTEINLQRYDGLIVMSQSDDDQPFIDYLKEKEFPFVVVNRHINDPAVINVTADDAA
                     GVMQAVDYGIGCGHTKIAYIGGKKNFRSTKEREKGVLKSLKKASIPVNPAYFLTGDYS
                     LESGLQNMEYLLSLSDLPTLVFCGNDDIAIGALRAVHLAGLRVPQNISLIGFDDSPVV
                     SYLNPPLTTVRKPLKEMCQEGTKLLLDLIEGRTIEQSKIELSTLLILRESINYL"
     gene            17694..19115
                     /locus_tag="IXT20_001363"
     CDS             17694..19115
                     /locus_tag="IXT20_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HAQ4859818.1"
                     /translation="MKKLTKELVKKNEAPVKVLQFGEGNFMRGFIDWQIQQLNNQQLF
                     KGNVAIIQPLEQGLGNMMNQQDNLYTVILQGLSNGEIVDTSEIITVVEQMINPYDEWD
                     AYLSLAENDDLAYIVSNTTEAGIQFVETDNLTDTPPQSLPGKLTAFLYRRFKLNKAGF
                     TIIPCELIDRNGERLKEIILRYAEKWDLEPAFVEWLEKENVFCCSLVDRIVPGYPRED
                     AGVFNQRHNYEDQLMVKAEPFMLWVIEGPKELENQLPFKQAGLNVIITDDMTPYRERK
                     VHLLNGPHTAMVPLALLAELETVEEVMKDPLFSAYVDQLFNLELIPMLSLPKDELAIY
                     ADQIKERFLNPFAHHKLEAISLNSVSKFSTRLLPVFKKYIEEQNQVPPLITVSLAALL
                     LMYRGDQVKPHDDEKTISEFTDAWSDNGTAIPRLLQNAALWGEDLSQIPNVTDTVQEI
                     AAKIDQKGVRSVLQEMKGTMVHA"
     gene            19108..20607
                     /locus_tag="IXT20_001364"
     CDS             19108..20607
                     /locus_tag="IXT20_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009224330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HAQ4859819.1"
                     /translation="MLETLQPTMKLNRRDSVVVALTPIPKQTQLTIDQQTITTLEDIP
                     QGHKIALGDLKAGDNVIKYGYPIGHVTTDVTAGQWLHTHNVKTNLSGELDYRYEKDVH
                     PSHYLFENRTFQGYLRKNGKVGIRNDLFLVPTVGCVNGIAELIVKQFKEKHPDLGQFD
                     HITILKHPYGCSQLGKDHQNTREILADAVNHPNAGGVLVFGLGCENNTVPEFKKILGE
                     YDEERVKFLVAQDVYDEIEQGVALLEELLTAAENDQRISVPLSKLNVGLKCGGSDGLS
                     GITANPLLGAFSDYLIAQGGSTILTEVPEMFGAEQVLMARAENEEVFDSIVDLINDFK
                     QYFLSYGEPVYENPSPGNKAGGITTLEDKSLGCTQKSGSSPVVDVLQYGEKIRKPGLS
                     LLQAPGNDLVAASALASSDCQLVLFTTGRGTPFGSYVPTVKVSTNTTLFERKGHWMDF
                     NAGVLLEQPMEVILESFVQKIIAVASGEETKNEQNDVREIAIFKNGVTL"
     gene            20646..22040
                     /gene="uxaC"
                     /locus_tag="IXT20_001365"
     CDS             20646..22040
                     /gene="uxaC"
                     /locus_tag="IXT20_001365"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HAQ4859820.1"
                     /translation="MFLSDDFLLKDDWAKKLYHSYAKNMPIIDYHCHLDPKEIYENNN
                     FSNLTEAWLGGDHYKWRLMRACGVPEEKITGNASDFEKFLAWCQTVPKIIGNPLYSWS
                     HLELKRFFGIDLLINEENAEKIWTQANEILAAPDFRRREIAKKSNVEVICTTDDPADD
                     LHYHQLLAHEEPDLKVLPSFRPDKALNIEKEGFNAWVTLLEEAADQTITTYQELIEVL
                     GQRIEYFHQHGCRVSDHALDILRYKEADETVLEEIFQKARANKLLSSDEIDAYRTETL
                     IHLTHFYHSHNWTMQLHIHAYRNCNTQMLEKLGPDTGYDGMNDLSLTIPLQKLLNRAE
                     ETDQLPKTILYSLNPNDYPAILALMGCFQKEQNGKLQLGSGWWYNDTRAGMRDQLTQF
                     ADGSALGNFVGMLTDSRSFLSYTRHEYFRRVLCEFLGEMVMRGEAPEDEQLLGSLVQA
                     ISYTNAKNYFGFFA"
     gene            22199..22882
                     /locus_tag="IXT20_001366"
     CDS             22199..22882
                     /locus_tag="IXT20_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnogalacturonan acetylesterase"
                     /protein_id="HAQ4859821.1"
                     /translation="MPTIHIAGDSTAAEKDENKRPETGWGEKISAYFSPEIQINNQAK
                     NGRSTKSFIQEGRLARLASQFQPNDFLFVQFGHNDQKIQEERGTLPFGSYLKNLQIFF
                     ECAKKANVQMVLLTSVTRRDYLENVTLNPDILGDYPKAMRAFAEKHHIPLLDVFSRSQ
                     ELFQTFSKEETKKFYLHLIPDTCKNYPEGLKDNTHFSPEGADKVARIIVELIKESRLP
                     LANYLQKGV"
     gene            22884..23720
                     /locus_tag="IXT20_001367"
     CDS             22884..23720
                     /locus_tag="IXT20_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HAQ4859822.1"
                     /translation="MFPLNLGIRAHDLDVRNREEIVQKVSQLHLSHIQFAPQKAFPEQ
                     LKLTDMTLGSAAYFGEYFQKNNIELSILGCYINLSSKNQEVRAQALATFKQQIALCSA
                     YQAKMIATETGSVSVGYTKENFTEEAYQIARNSIIELVAHAENFGVTVAVEAGINHPI
                     YNYQLAKRLIDEVASPNLKIILDCANLMHKENHADQEKVIRGALDNLHGYITALHLKD
                     YIMENGKIRMVPVGKGCLDFRPVLHYIKYERPLMYATLEATPEIYVPEAIEHLQFIYQ
                     DC"
     gene            23731..25485
                     /locus_tag="IXT20_001368"
     CDS             23731..25485
                     /locus_tag="IXT20_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ4859823.1"
                     /translation="MEERIQKSYQKYHANAFQTLLHLYQGNYWRFAMSGLFFIIKHLP
                     SWLMPIAIANVINAASSKDSSQLQAIYFNAFLMACLIGQNILTNYFHVKYHSTSIRQV
                     EAGLRATLIKKIQELSIPYQKELQSGRIQSKIIRDVEAIQTLSSQVFVSGLNIAVNLF
                     VALGITLYKSPVVFMFFLLTVPLAAFVTLFFRKRIRQSNSDFRQEMEETSAKVIEVIE
                     LAPIARAHGLEKHETTRLNKHFTQIYEKGFHLDILQSVFGSVSWASFQIFQLICLIFT
                     GILALQGRIQPGDVVMYQTYFTTIVNSVSGMITLIPTISKGMESITSIGELLIAGDVE
                     TYHGKKKIQHLDGNFRFEDVSFLYPDSLKPIIDHFSLEVKAGETIAFVGPSGSGKSTL
                     LNLLIGFIQPTSGKIFLDDQPLDELDMRSVRNYLAVVPQTTLLFSASIKENITYGLKN
                     VSKERLDEVIEAAQLSSLIDQLPDGLDTLVGEHGNKLSGGQKQRISIARALIRQPKII
                     LLDEATSALDNQSEKKIQQALNYLTETPTTFIVAHRLSTIKEADKIVVIDEGKIVEIG
                     TYEKLLAQKGYFYRLKNE"
     gene            complement(25761..26102)
                     /locus_tag="IXT20_001369"
     CDS             complement(25761..26102)
                     /locus_tag="IXT20_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HAQ4859824.1"
                     /translation="MSNIYDSANQIEREIRELPEFKALKEAYEKVQANEEAHKLFKDF
                     QAMQIELQEKQMSGQEFSDEDAMKAQEMAVKIQSEEVINDLMQKEQGFSTIINDLNRI
                     IMTPVRDLYSE"
     gene            complement(26170..28341)
                     /locus_tag="IXT20_001370"
     CDS             complement(26170..28341)
                     /locus_tag="IXT20_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HAQ4859825.1"
                     /translation="MDFQSILEKIKAALISFWRWIRPYLGQFHRWRKRIWKKYHVNKL
                     ILLIGLICVLVTSIYLFILAKQANVETLKSGLSQSTVIYDKKNEEAGTLYAQKGTYVE
                     LNAISPYIQDAVISTEDRNFYEHHGFDIKGIARAAVRMVLSGGTSGGGGSTITQQLAK
                     NAYLTLDQTFDRKAKELFLAIEIEKKYSKEEILTMYLNNAYFGNGVWGVQDASRKYFG
                     VDATNVTLSEAATLAGMLKGPGIYNPIDHPKNANDRKNTVLSVMEENGKITKEQEQAE
                     SQTDISAYLNDTYDNSDSGYRYPYYFDAVIDEAVNEYGLDEEDVMNKGYKIYTALDQD
                     YQKQLEATYQNSALFPANASDGAMVQSASVALDPNTGGVQALVGRRGDHVFRGFSFAT
                     QMRRSPGSTIKPLSVYTPALEAGYKPSSILKDEPQSYYDAHNFDGTYQGEVPMYQAVA
                     QSLNLPTVWLLHEIGLQKGYDKAEEFGLPLAKSDKYYGLALGGLEKGVSPLIMAGAYS
                     AFANDGQMYTPHLITKIVDSTGAVIVDNTNKKPKQVISKETADEMTSMLLGTFSNGTA
                     AAANPAGYTIAGKTGTTETNFDATKANDQWMIGYTPDVVISTWMGYEESSKLHYLEGT
                     SGNVVGKVFKSAAEGILPYTSKTGFSVADAYATGGQVVAADQVGNTGNSNGESGNWQD
                     NLNQYGEQAKEGLKNFGDMVKEGVQGIGEAAKDLWRKYQGE"
     gene            28552..29409
                     /locus_tag="IXT20_001371"
     CDS             28552..29409
                     /locus_tag="IXT20_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HAQ4859826.1"
                     /translation="MKITITLPMNHPETTIRDLLEKEWLVPRKVRHFLRIRKNVWINQ
                     QPALFHHEVKGNDQITLLFEETDYNYQEVQLGDAKHIRVLYEDEHLIIVDKPAGMKTH
                     PNEPTENSTLLNHLAAYLDTKQQRPYVVHRLDKETSGAVLFAKNPFVLPILGRMLEQK
                     MIYRRYQAIVWGEIAKDMTITNKIGRDRHDRRKRVVDDKNGQSALTHVTVNKVLSEQT
                     QVYCVLETGRTHQIRVHLAHKGHPIVGDPLYQNRTAERLMLHALELHMIHPFTQEKIV
                     GKALPGLWE"
BASE COUNT         8840 a         6048 c         5212 g         9359 t
ORIGIN      
        1 acgacttagt tatcttatca cgtctaaaaa catgtgtcaa ctcttttttg gaattttcat
       61 aaaatgtttc aatgaagcgc gacaacgttg tttaatatat cattaaaaaa aataaaggtc
      121 aagctaaaat attatttaag cttgacctta ttataatttc aacgattgtc aaaagtattg
      181 tttgcagctg tgttgccagt ttctatagat aaatcacagc ttttgaaaca taagttttgt
      241 actgcttcga ttcgcccgaa ttcttttgca gttatttggc aatgagaaaa gcagacatct
      301 tctataggct tttcaggaaa tgctactaaa tcaaaagctc gggaaggaag tgagtaacct
      361 ggagaaaggc atgcctgtac gtttttgatg tgtatgtctt tgactttcgt catttgcttt
      421 gattcaggta tttcttcagc cactgcttgc caactttctg gcatttgagc aaggtcattg
      481 ctattttttc tattgtattc attatgccag tccattatcc aagaaaaagg aaattgtaca
      541 tcaatcatat tcagattttc taccctgata ttttcgatca ctccccctct ttcttttgaa
      601 gatttcattc gaataccaca gtctgactga aaaaaatcga tatcgtgaat atatacgtca
      661 ctaactccag cactaacttc gctaccaata gttacaccgt atcctgaacg gattttacat
      721 ctagcaacct cgattcttga tgattttcga ttgactcttg caccattgcc atctctgcca
      781 gacttaatag caatacaatc atcaccacag gccaaatcac attcatagac acgcacattt
      841 gttgaagaat caatatcaat cccatctgta cttggtccat cattgttgcg gatgattact
      901 ttttctacta atacatcgtt agaataagtg atttgcaagt tccaaaaacc tgatttctga
      961 aaaatcaaat cacgaacaac aacatgttct gcatgataca gtaaacaagc gcgtggacgt
     1021 ttaatagcat aatccgcaat ccaacgaagt cccttttgat cgtattcagc tcttgtcccg
     1081 cttttttgat cttttcccca atacagctcc caccaatggt ctccttgacc gtcgattatt
     1141 ccttttcctt ctataagaat atctttcgcc gagagaacat tcaaaatcgc tgctggccat
     1201 ttcatttcta cgccggctac tcgtgtatcg atttctggaa atgcttcgat atctgttctc
     1261 cccttgagga ctgcaccttc ttcaaagata agatgactct tatcttttaa aaaaagggct
     1321 ccgactaaat aaatgccttt tggaataaat acaggtaagt gttctttcgc accttgatcg
     1381 attgcttttt gcagtattat tgtatcatct tgttgagtgt cgtttgtttt aaagttcaaa
     1441 gcattgataa tccctttttt tgtccgcaat acgtttctcc ctatctttca ttgatttttt
     1501 tcaattcttc accaataaat acagtttttg aacgattcag tgggtaacct actagcaaaa
     1561 tgattgcgga tacaaataag cagaatgctg gtaacaagtt tgccatcgta taaatccttt
     1621 cttgtactaa aactgattgg atggctccac ctgttgtaga cgattggtaa ccaatgagtg
     1681 ccagcataaa tccgcctacc gctcctgcac atgcttgtcc tacttttctc gcaaaagaat
     1741 ttactccata tactgttcca tcttctctta agcctgtctt atattgatga taatcaatca
     1801 cgtcagtaat gaatgcccag accatcaaat tgaataaacc agatcctaaa gtagcgataa
     1861 ataaaagaat cagatatacc catgcatttg ttatatgaag gaaatacatc agcaagtaca
     1921 tacatgaaga agcaagtaac gcaaatacgc ttgcttcctt tttaccaaat aattttgtca
     1981 gctttgtagc aaatggtgct aaaactacta ctgttccgta agtaaataaa agagcaacag
     2041 acattgcttg tttattatgg aagtaatcat tgaataaata agtcatattc gtcccagcta
     2101 aaatctgatt gatcacaatg aaaatgtcaa caatgactaa aataatcagt gctcgattag
     2161 ctaacaaacc ttttaccaac tgagaagcag atactttttc gtttttgaca actcttactc
     2221 tttcagtagt caatttacaa gttaacatat atgcgacaaa tgctattgca gcacatccga
     2281 ttgaaatcca aaagaaatga ttccctgaaa cgacttgttg tccactcttg ttatgggtat
     2341 acattaaaat cggaatgaag aatcctgtag ttgatccgcc aatcgcagaa ccaatgcttc
     2401 ggaacgtgga caacgaggtg cggtcattag gatttgagct aattgctgct gccattgctc
     2461 catacgggat attgattgtt gagagaaaaa tcccataaac aatgtatgaa acaaaaatat
     2521 aagcaatctt caccgtcatt ggaagatgtt ggacgaatgg caaaaacaaa atgacagtca
     2581 ctaagcaaaa tggatacttc atttttctga tccaaggaat aaatcggcca gtttctgtta
     2641 actctgagac atcacataat cttccaacca tcacatcagc aaacgcatcc aaaattctcg
     2701 aaatcaagaa taacgtacct acgattgccc ctgaaacgcc aagaacattc gtataataaa
     2761 tcatcaagaa actattgact aaacctaaaa taaaacagtt acctagatca ccgaacatgt
     2821 agcctatctt atcggaaatc ccaaattcct ttttttcttt ttttattgta tttgctgtaa
     2881 cagcattttg tgaaagcatt tgaattcctc ctacctcttc tagtaaacgt tttcaaaatt
     2941 ataactcttt ttttcttgtt tttatattca caatgttggc tttttattgc attatcttga
     3001 cagataaaaa acaaaaaata atctaaaaac attgatgcat aagattgata acttttttta
     3061 cataacaata tatacaaaca aaaaaattca ctaactagtg aaaataagtt agtgaacctc
     3121 atttattaaa gaattaaatt acagatgatc ttttccatca tacttgaaaa actcaaaatc
     3181 agcataactt cctctttttt tatttagatc atgcactgct atcccgataa aatttccagt
     3241 aaaccctcct gagagtatac acatgttttt ttgcggacct actttcttcc attcttcagt
     3301 tgatacgtct ttcaaatgaa actgtgctgt ctctttcttt acagtgactt tcaaaaaagt
     3361 cttcttttct gtgagtggaa tcgtccaatc ttctatttgt gctactccgt tttctgattc
     3421 tatcatacgt aggcattttc ccttgtcctc attccaagta acataacaat aaatataatt
     3481 ggattcattt aagtacagta ccagtccagc catctgatta aaattattag gcatgtattc
     3541 aaatgctgtc tccgcatcaa aagtaaaatc cgtttgccga atagctaaca aatgatgttc
     3601 gaaggtagat tgaagagatt caccggaaat cagacgtaaa aaacctggtc tacattttgt
     3661 atcacaccaa tcagcatttg gcaaaatgcg tctggcattc cagttcttct ttagctctcc
     3721 ttcaaatgta tccataaact gatgatcttt ttcttgttgt acctcttcat ttgtctcaat
     3781 cattatttct tccatcggag ttgtctggct atctttaagt cttaaccaat cgtcggacgt
     3841 ccaatacact tcttgaatag cggtttctct tcccaaaata gccgcaccgt ttaatggtct
     3901 ggtacaaaga tatgtcatgt accatcttcc tgacggggtt tgtactaagc tcccatgtcc
     3961 tgtacattgc aatgtagaag caggtttgtc actagctgtc agcataggaa aaccaggatc
     4021 aagctcgtaa ggaccagtaa tattctttgc tcggcaaact gtaactgaat gtcctcttcc
     4081 tgtaccgcct tctgccgtca tcaaataata atacccgtta cgaaaataca aatgtggtgc
     4141 ctccgttttc gctaattctg tacctgcaaa tattttatag atgggaccat caagcttctt
     4201 cttctctgca tcatattctt gtaatacgat accgctggat ttattcggtg tgtccagacg
     4261 ataatcccat agttcattga caagccattt ttttcccgtt tcaagatcat ggaacaaaga
     4321 aggatcaaag ccgttactat ttaaataaat tggttcggac caaggacctg tgatctcaga
     4381 tgcagtgatc aaataattgt ggacatcttt aaacggacgg cctgcacttt tgacatctgt
     4441 gtaaacaaga taaaacaatc catcagcata actgatctgt ggcgcccata tacttccatt
     4501 tgtaggattt ccttgcaaag atacttgagt ggtcaaaata tccgttgtat gtgaccagtt
     4561 gactaaattt ttcgactcat atactcggat tcctggaagc cattcaaaag aggagacaat
     4621 aatgtaatac cgctctttca ctcttaatat attaggatct ggattgaatc ctcttaaaat
     4681 aggatttttc actttgatag tctgcatgat tcaggctcct tttctcattc ttactcgata
     4741 taaactgaat gccccagctg cgaaataaaa aaatgcaaaa ggaaacgcaa taaactgagt
     4801 gccaatcaac aatatgaatc ctgttataaa atacaaacca gtggcagttg ttaagtattt
     4861 tcctttagca gtcaaaaaca atgctactgc taaaaaagca agtgaaataa acaacacaac
     4921 ggcaaacaga ccagtcatgt cctgtaataa tccaaatgta ttttctaaag acaaagttga
     4981 atgatctaca gcaagcgact gataactttt tttaaactct gacgcagaca tattacttaa
     5041 agtaactgct aacccgccaa agaaaatcaa agatagactg ccaccgacca ttcctagcaa
     5101 tagttcccat tttcttttca tttctgaacc ccctgctttt ctaaatgtat ccattcaggt
     5161 cttttgatcg atgtttcata attgttatta cgttcagaga atttgacagt ctgttcattt
     5221 ttcttgtcat cccaattatc ccaaccagtt gggtgaacat gcccacctat cttacacgct
     5281 tcaaataaga catttgcata cggacgccac ggtcttccta aatagacgtt tctagtatca
     5341 ggttctgcag tcaaggtaca gctttcaaaa cgataccctg ttttttgatt tttcggcgta
     5401 gatgctgctg tgatgtatcc aggattttta ctggctaagc tatggatatg acactgatga
     5461 aaaacggcat ctgccccacc aaatatgaaa tccaccgttc cttcaatcca gcaatattca
     5521 taggtctgac gacaatactc aggattggca gaagccggtg tacaaaaagg agtaccatct
     5581 ttttgcaaat caggcaacgg acccgtgcat aaggtatcct gataaccaga tagtcgacag
     5641 ttttttaaag atacgcgatc tcctgaattg aataaagcaa cagcctgtcc taccttttct
     5701 cctggacctg ctgtattttc gatgtgaaga ttttctaata cgacattcga tccttctaag
     5761 aaaaaagtag cacttcggaa agtccctaac tggctaccgt caagatactc ctgttttgca
     5821 aaacgattac cggagataac tactgttcct gctcccacaa gtttaaaatt ggataatctg
     5881 ctttctatat attcatcata tcttccttct tttactatca agacctttgg atggctatcc
     5941 ggcaggcctg ccaaataatc gattccttct tggatagttt caaaatccgt cggggtatct
     6001 atagcattct ttccaattat cagtttgtct ttcatcctca taaatgctcc cttatgaatt
     6061 gtttccaaat atttctcatt tcttgtgttt ccatatgacc gcctgttaaa gaaaaagaag
     6121 aaaagttttc tggactaccc attgattgat actttttgtc cagccctttt ctcagcttca
     6181 gtacgccttc gtccaagcac atcgtatcgt tttttccaac taagctaaga cgagggcgcg
     6241 gagcgatttt ttcttggatt gctaacgttg tataagctgt caatagattt ggcacatagt
     6301 aataaaaccc atgatgatct aggcggcgat gtagcaaaag tatttcgatg tccacctgtg
     6361 ccgctagatc aatacaaact ttgacacgag aatccaatgc tgtcacccac cagcttaata
     6421 agcctcccat agaaagacca atcgttgcga gccgttgagt atctgtgtct tcccttgtct
     6481 ccaaataatc gatcaagctg atcacatcat aaatacgcat tccccataac gtttttcctg
     6541 ttaataaaaa ttgtttgaac aactcgcttt cagaaatacc tccgcgttct tcaaatcccc
     6601 aagcatcaat cgaccaaaca gcatatccca ttcctgtcag tacttctgca aaagatggag
     6661 aagctaagta aggagcacca tggatcaatt cacttttccc gactgaaaaa tctccgccgt
     6721 gagaatgcag gtaaaggacc ggcggaaacg gtccttcttt ttctggataa gcatagaggg
     6781 ctggtactgt ttccaattca tttaagtaca attgatagta ttcaagcata aaaccgtttt
     6841 gtttttctcg gtataatcgt tttccactag gggtatgtct gctagatgga gcacctaaaa
     6901 aatcattcaa ctgcataagc aaagttttta cgaacaacca gttcatccat attttctgat
     6961 tgatttgatt ggaatttctc aatactgatt tctttagtat tttctaatag aaatgctggt
     7021 ccctcgtgat tttcaatcga catctggtta aaggaaacct tacccgcaaa gccaatataa
     7081 aatccttgtc tgctcatttc ctctattcct gtcatcatag caggaacacc gggaactgca
     7141 tcttgggcca tcgagacatc gatattgtca aatgtgattt cttgtatgaa ttgttccgct
     7201 aatccataaa taaaacctgc agaagcatgg acattccttg ccgtgatatt cgaaaaatgg
     7261 atccttctga atgctggtgt ccgttcatct atcggataag cctttttttc ccatacatac
     7321 ggttctttcc ctcgcggacc acagaaataa taaagattca gtataaatgg acacatcaca
     7381 ttatccatga ctatattgga tactcgaata tcttcaacga tcccgcctcg acctcgtcta
     7441 gatttcaatc gtattcctcg atcagtttct tggaagatac aatttgaaat ggtaatatta
     7501 cgaatactac cactcatttc acttcctaat acgacaccgc catgtccatg gaccatcgtg
     7561 caatttgtga tcgtgatatt ttcgcaagcg atcctttcgt acgtatcttc agtccctgcc
     7621 ttgattgcaa tacagtcatc tcccacgtca atatggcaat tactaatacg cacatttttg
     7681 catgattctg gatcaattcc gtctgtattt ggtgaatccg ctgggttcaa gatcgtcaag
     7741 ttatcaaacg ttgcattact gcaaagaatc gggttgatcg tccaacttgg tgactggatc
     7801 aatttaatat ctttcacagt aattcgatga cagttatgga aactcatcaa tttaggacga
     7861 ggataagcga gattatctgg ttcatttcta aaagtatgcc accatttttt tccatttcca
     7921 tctaatgtac cgaaaccagt gacagagatg ttctctacat tttgagcata gatacaggaa
     7981 gcataaactt ttcgatggac tccttcccaa cgtgaatgaa caacaggata atcttttgga
     8041 tcgtcagaga atttcagaac cgctcctgcg cttaaatgaa gttcgacatt gctttttaaa
     8101 aataaagctc ctgttagaaa ttcacctgct ggaacaacta cggtcccgcc tccgtcactt
     8161 gctgcagcat caatggcttg ttgtattgct tcagtattca attcatcaat tgatgcgcca
     8221 aatgtcaaaa tatcatacat atttataatg cctccacttc aatcaatgct tgcaagaatg
     8281 caccaacgcc tttttgatca ttacaaataa ttggttcact gatgtagtaa gcatatgaac
     8341 catctcgctg atctgctccg cctaaccctg ctacttgaca gtttttattc aagttcagcc
     8401 agccttcttt tgtttccaga acaaattcat caagtaatcc ttgatgtgat ttcttcactt
     8461 gtggaagcca ttcttctttg ttcagcagtc ccaaacgtat tcctttagcc attgcgtaaa
     8521 caaacatact actgccggaa gcttccagat aatttccttt ccgttttgtt tctgttgtta
     8581 cttgatacca tgtcccgctg ttttcttctt gatatggaag taatgcagcc agtgtctttt
     8641 ctagaatttc tttcagcaaa gaacaatctg tttcttcatg aagtaattca taggtatctg
     8701 ctgcagccat caaataccag ccaatcgatc ggctccaaaa atgatgggat agacctgttt
     8761 ttggatcagc ccatggttgg accttctttt catcccatgc atgaatcagc aaatttgttt
     8821 ttggatcgaa tagatgttta taactgattt caaactgtct caaaacatct tggtaatctt
     8881 tttcttcact aaaattctgg ataaattcag cataaaaagg agaacccata tataagccat
     8941 ccaaccaaat ttgataagga taaatttctt tatgccaaaa agctccttcg gatgtgcgtg
     9001 gatggtttgc caactgctgg gaaaggagtt ctgctgcttt tttatatttt tcttcgcctg
     9061 tttctttgta caataaaaag aaaagctttc ctgtatttac atggtcgata ttgtattctt
     9121 caacgctgta gccacggatc gttccatctt cttgcacgaa atagtcgata ttgtctttta
     9181 tgaaatcaaa ataaattttt tcttgggttc gttcccaaag taattggaac cctttaagta
     9241 cgacgccata gtcataagac cattttcctt tataccactt ttcttcatat aagcgaggtg
     9301 ttctttccat gatacttttt gctaatttct ttgaccagtt ttccattatt tgctctcctt
     9361 taaaaattaa aataggattt gggacaaata ccaagaagta tttgtcccaa tcgtctatat
     9421 tactgtgccg ctgctttgta tgcttcctcg tactcttgtg tgattcgatc gcctccttgt
     9481 gctttccagt cttctacagc ttttttgaat ccatcttcgt caagctgtcc catgatgtat
     9541 tgcaccgtag catcaatgac gattttttca atttctgatc ctacttcatt gttcgtcggt
     9601 gaatccaatg gcacagaagg atccattaca gcaaattctt cattttcttt gatcaattga
     9661 ttagagaatt ctttttcagg gtctgtatct ttgaaagaga acgtcacttc actaggacga
     9721 gagcttgata acggttgcac ctcttgttgc caaagatcca tatcgataaa ttcgatagaa
     9781 ccatcatcat tcaattcata atgcgttcct tcgataccgc cagtcatcaa tttatacaca
     9841 tcttcttcta aaagatcatc aacaaattga agtaatttct tcaattgtgc ttcgtctttt
     9901 acttgagatt tgggaaatgc tagcaagccg ccaataccat ttccttctga ccaaacatga
     9961 taaccgccgc cttcaccatc tgagatcttg tttacaggca ctaattccat atcatcttga
    10021 ataccttgac ccatcgtctt cagattcttg atatccacca ttccagtgta gattccagtt
    10081 cttccttgtg caaattgttc ttgctgatca gtcttttggg taactgcaaa gtcttgtgcc
    10141 aaatagccat tctcaaatag atctcttgaa aaattcaaag ctttcatata ctcttctgta
    10201 tcaaattctg gagtgaattt gccattatcg tctacagccc agccatttgg tgctccaaaa
    10261 taagaagtga aaagtttaaa gctggtataa cgaatatcac taggcgcacg atcgccaaaa
    10321 ccaactgtgt cgtccacacc atttccatca ggatcgtttt ctgtaaattg tttagctact
    10381 tcataaagct catccatggt agttggaaca tctagtccta gattatccaa ccagtctttt
    10441 cgaatgacta agccagcacg agcgtagtct ttttgtatcg gtacgccgta caaggcaccg
    10501 tcgatactcg cagcttcgat tctatcttca gaaatttgac ttaaattttt atagtctttt
    10561 aaatattctg atacatccca gaatacgcct gattttaacg cattgcggac agaagagttt
    10621 ttcaacatcg caatttgaag tgtaacgata tctcctagtt catcagaagc caatgcagtt
    10681 gttaatcttt cttcttttga agcatcagga atccagttga atgtcagttc cgtatcggtt
    10741 ttttcttcga tcaagttcac aatcgtgtct gtaggcggtg aagcatgatg caaaatattt
    10801 aaccaagtga ttttagcttt tccatcttct gtctctgaag aactgttact tgaactgctg
    10861 ccgcatgcgg caagtgctag aattcctgca aaactaagac ctaattttac gagcgttgct
    10921 tttttcataa attttctcct tcactgtttt aataatattt ttgttttctt ctgttttttc
    10981 tgtatatgag ttcttccatt ccaaatggca aaatatagaa tttgctgtcc acgtgactgc
    11041 ggaaaagaag agactgccac cgataaaggt taaaacacct ggcattgttt ggatgatcat
    11101 cagataaatc cctaccatac ttaatatcaa cactaatgag taatgaagat gtgagaatcc
    11161 gaataaaaat gccattttca ttttgaaaaa atatcctttc caatcataat gagccatgat
    11221 tgggaaaata tagagtgctg agagcacata tatgaacgca gcaatataca aagcgatcct
    11281 aagataccaa ttgtgtatga aagaaagatc cacaagtaaa acaatcccga tggcgccata
    11341 tatccagctc atcagcaagg tttcacgata gttttcttta aaatatgtga agtacgtttt
    11401 cgtcaaagga aacgtttctt ttgagcgcag ccattttcta atcactgcaa ccatcgcata
    11461 cgatgcagga ccgaaagtaa aaactcccaa acctgcgatg actaaaccgc cccacagaaa
    11521 attcaaataa accaactgta aaatacgatt tagccttccg tttattttat ctaatgactg
    11581 cagcatatct ttccctctct ttctatctta gtaaacgccg tcttctccta gctttttggc
    11641 aatcttatta gctaaaatta ccataaacaa tcctacgacc cctttaaaca atcccattgc
    11701 tgtactgaaa cttaactgtc cattcttcaa tccagctgta taaatatagg tgtcgaaaat
    11761 ttctgcaacg cttcgattca atgaattcaa taacaagtac atatgctcga accctaagtc
    11821 taatgtctga ccgattttta gaatcagcaa ggtaatgatt actggacgaa ttgccggcaa
    11881 tgtaacatgc catgttttgc gtaagcgtcc tgctccatcc atttctgcag cttcatacaa
    11941 ctgtgtatcg actgcagtta tcgctgaaag gtagatgatc gtagaccagc ccaactcttt
    12001 ccagatcact tgtccgatat atactgtgcg tgtccattca ggggatgtta gaaaactgat
    12061 tttttcaaat cccattgttg caaggaattc attcaatacc cctccgtcaa cattcaaaaa
    12121 tacataagta atcgaaacaa tgattaccca agacatgaag tgagggatgt aaatgatcgt
    12181 ttgaatcccg tttttcaatc gtttgttcgt tacttcattt aatagtaaag acaaaatgat
    12241 tggcatcgga aaaaacacga agatatttaa tccgaacaag atcaacgtat ttcttaataa
    12301 tgtaaagaat gtcggttctg taaacaaacg aatgaaatgt ttgaaaccga cccaaggact
    12361 tcccattatt cctaaaaatg gttgataatc ttgaaaagca ataacgagtc cccccatagg
    12421 aagataccta aaaatcaaga aataaaggac tcccggcaga atcatcacat ataaaaattt
    12481 attcgttttg attcctgcaa ttattttttt tcgtcttctg agcttttctt cttttttcga
    12541 tagcgtcagt tgaaatggtt cttgtttctt tacactttcc ataagcccct ccttagtttt
    12601 gtgaatgttt gctgcttatg acttcatgat agataactat ctgaattacg tatataatca
    12661 tttttaccgt aagtaaaaac gcttacatag caatgatttt tgtgctatac aaaacgataa
    12721 agtgtatttt ttcacaaata aactacgcat agcaaacctt ctattttttt ctatctgaca
    12781 cttagcatag gaaatgatta tatacgtaat ctttcaccga tgataaattg aacatataga
    12841 aacagaaaca tttaatttat tttgaggagg agcctatcta tatgaaaaag aaagtctcta
    12901 taggagaaag aagttttaca cttttcaaca atacgtttct aattttgcta gctttgatct
    12961 gtatcgttcc ttttttaaat atcatcgcta catcttttgc ttctacacaa gaagtcgtcg
    13021 cgaaaaagtt tatccttttt cctactacct tttctttaga cgcgtatcgc tacattcttt
    13081 ctacgccgac catttttaga gcacttgctg tttcgattgg tgttacaggt gtcggaacga
    13141 tcgtcagtat gtgtgcaact tcactgatgg cctacggtct ttccagaaag tatttgtttg
    13201 gtcgaggttt cgtcaacttt cttgttgtat tttctatgtt gttcagcggg gggatgattc
    13261 caacgttctt agttgttcgt tctctagggc tggtcaattc ttactgggcc atgatcctgc
    13321 ctgtggctgt taatgcaatg aatatgatca ttatgagaaa ctttttccaa gccttgccgg
    13381 atagtttgga agaatcagca aaaatggatg gttgcacaga ttttggtgta tttttcaaaa
    13441 ttatgctgcc attagcgtta ccgtctattg ctactatctc gcttttttat gctgttactt
    13501 actggaacac ttatatgact gctattttgt atatcaacga ttcatctaaa tggccgatac
    13561 agattctttt acgccaaatc gtcattgtat caagtgggat gcaagcagaa agtagtgccg
    13621 ttgatgtgat tccaccagca caaacagtta aaatggctgt tatcgttatt gcaacagtgc
    13681 ctatgctgat tgtttatcct tttgttcaga aatattttgt caaaggagca ctcgtaggtt
    13741 ctgtcaaagg gtgattatgt actgaacgca aaatggtagt tgtgtcagaa gtggtcagct
    13801 tcaagaaata agaaggcatt tacgaaaatt gcttcttaaa tttttgtgaa atttcggctt
    13861 atttttcgaa gaatctactc ctggaacacc gtgtatcaga ggttgtgaaa gagacgttca
    13921 gccttgagcg atgataaaaa ctctcacaaa cattgcgacg agtaacccac aggagcaata
    13981 agaagcgatt ttgaaaaata gcttcccata tttcacaaaa ttatgcctta ttgtcgatag
    14041 cctgtctctt gaacgccatt tattcgtaaa aaagaactgt gacaagactt ttgtcacagt
    14101 tctttttgcg tgcttagcct tgaaatttaa ctatccataa atacaaatac tagggttatt
    14161 ctaacttctt ttattgcttg aatcagtcca caagaaaaac agcctcttga ttcacaaata
    14221 tttttttcga tattctcccg gcgtaatcga ttccttcttt ttaaagaaac gaataaaatt
    14281 ttgcggattt cgatattgca atctttctgc aatctctttt acggagaggt tagtctcttt
    14341 caaccatttt tttgctacct ctaaacgatg attttgaaca aaatcaccaa atttttctcc
    14401 tgtttccttt ttaaatacat tacttaaata gttagggtta taatgcaacc tatcagcgat
    14461 cacatctaaa gacagatcac gatcatattc tgtgtagacg atatgcataa tattctccga
    14521 caaattttta aattcatgat cagtgattgc ttgagagcat tctgtgattg gcagaactaa
    14581 ttgttctaaa acaagttttt ctagttcttt gggatgataa ttgttcagtg cttttacata
    14641 aatttgtttc atgttatcaa agttttttga atctactccc aataactgtc ctaattgaac
    14701 gagttcattg atcaagcgga ccgaaactac ttcccgactc aaaggatttt tattttgtgc
    14761 aaacaatgca tcaatcaatt catgtactaa attcttcact tcctgatttc cagaacgaat
    14821 ggcttcaaac aatcgatttt gttcttctat cggatatcta ataagtgttt tttcatgagc
    14881 agcaggaacg atttcctgat aaaaaataat tgaacttgga ccagtattta cacgataata
    14941 cagggcttct tttgcccgat caaccgattg cttgctttca ttcagcgttt cgaaccggtt
    15001 actgatgcct atactgatag ttaaattcaa gtattctttt acacgttttt gaatgagtcg
    15061 gcaatactcc atgacttttt tgcttgcttc tgcatcatct ttatccattc gataaatagt
    15121 ggcttgcatt tcttcattca aaacgatcgg cagcattcga ttttcctttg gcacaatttc
    15181 cgtgatcatg ttgttgatcg ctaaaagtag caaatcacgc tccccaccgt gactatcttc
    15241 taacagatcg atttgaacca aagctgtata atagcagtca ttttgagaac tataaccaaa
    15301 ttgctgcaac cgctgatcta gttctcgcct ttctactcga tttctaaata aactaagtac
    15361 aaatagcgtt tccagctgtg gcttttgggt aactaagtgt gcacttaacg cttctttttc
    15421 tcctatgatc cgactgactg attgagcgac caaatctagt tctttcccac tgaatccttt
    15481 tgatttaaga ttcagacgtt cctgtatttg agaaatcgga cgggcaaatc tgtccgaaaa
    15541 taagtaggaa agaagaccaa tgagtcctac cagtgttaaa gacactgcaa ctagcccgat
    15601 tctcagattg cggatcattg cgccaattgc actagagtca acttctaatc tgtaaatcca
    15661 attattataa tctgatttca gtactatata cgttttatct ttttctctta tcactttcag
    15721 ctgattgtaa tctagatcca cgtccgttaa tacagttggt tgctctttat tttttactgt
    15781 ctgagtagta aaaaggttgc caacttcgtt ttcaatagaa agtatgtctt ctccactttc
    15841 accaacaact tgttctattg tatgtttctc tatcgtagca attcccaatg catatttttc
    15901 tttttcaaac attggcaaag aaatcaccat ttcgattcct ttgtttttct tttgccaaaa
    15961 taaattattt ttgttattta tgtacttctg ttgatacttt tcaatttcct cttcggtcag
    16021 ttgactcaac gatccattga ttactcccca tttaccagcc aaactgacta aatcataatt
    16081 attatcatcc ataacaatcg tttctataaa atttagttct ttcctgattt cagaataagt
    16141 ggagtaatct gtatatgtca gcggatgctt gaatatgtcc gagaaatttt tggtcgaacc
    16201 atacgtagaa aacgaatatt caatcgttct aattttccat tcaacgttag ttttaacttg
    16261 cctgagcaaa ttctttttgt ctgttaaata actcgattcg atcgattcat aggtattctg
    16321 gtaaatgaat aaactaaatc ctatgacaag tacggctcct aataaaaaca tcggtaaaaa
    16381 tattttataa aatactgtac ttttcttcat tttcttcctc tcctcctttt aaaataaaag
    16441 gctttcattt ttctgtaaaa ttgatactca tctaattaaa tgagcattcc ccgctttcaa
    16501 gaatagcata attaaaaata aaaatccaag taatttttaa cgtgtgcaat cttttcatat
    16561 caaagatcta ttgacttatc gtagttatcg taatatgatg aatgcgttaa caaaaagtga
    16621 aaaagtaagg aaaatgatgg aatggtaacg atcaaagatg tcgctaaagc agctggcgtt
    16681 tctcatacga cagtttctcg tgctttgaat aacagcagtt taataaaaaa tgaaacaaaa
    16741 caaaaaatac gacaactagc taaggaaatg aattattcgc caaactatag tgcaaaaggt
    16801 ttagtgaatc aaaaaaaata tctaatcgga ctatttttct caagtttgca taaaggaact
    16861 tcatcaaatt tcctagcaga ttccattgca ggcatccatt cagtattaga cacaaattac
    16921 agtttatctg ttgaaagtat cgatgttgtt gatttaacag aaatcaacct gcaacgctac
    16981 gatggactga tcgttatgag tcaaagtgat gatgatcagc ctttcatcga ctatctcaaa
    17041 gaaaaagaat ttcctttcgt tgttgtcaat cgtcacatca atgatccagc ggttatcaac
    17101 gtcactgccg atgatgcggc cggtgttatg caggcagtag attacggtat tggatgcgga
    17161 catacgaaga tcgcttatat tggcggaaag aaaaattttc gttcgacaaa agaacgggaa
    17221 aaaggggtat tgaaaagtct aaaaaaagcg agcatccctg tcaatccagc ctatttttta
    17281 acaggagatt acagcttaga aagcggcctc caaaacatgg aatacctatt atcactttct
    17341 gacctgccta ctctggtttt ttgcggcaac gacgacatag cgattggtgc attgagagct
    17401 gtacatttag ctggcctccg tgttccccag aacatttctc tgatcgggtt cgatgattct
    17461 ccagtcgtct cctatctcaa tccgccgctt accaccgtac gtaagccttt gaaagaaatg
    17521 tgtcaagaag gaacaaagct tttactagat ttgatcgaag gcaggacgat cgaacaatct
    17581 aaaattgagc tctccacctt gttgatcctt cgtgagtcga tcaattactt gtaaactaca
    17641 agtaatttta tataaaaatg ttaacgtgtg cataacacag aaaggaagtt tatatgaaaa
    17701 aactgacaaa agaattagtg aaaaaaaacg aagcacctgt aaaagttctt cagtttggtg
    17761 aaggcaattt tatgcgtgga tttattgatt ggcagattca gcagctgaat aaccaacaat
    17821 tgtttaaagg taatgttgca atcattcaac ctttagaaca aggtcttgga aatatgatga
    17881 accagcaaga taatttatat acggtcattt tacaaggact cagcaatggc gaaatcgttg
    17941 atacttccga gattatcacc gtagtagaac aaatgatcaa tccttatgat gaatgggatg
    18001 cttatctatc tctagcagaa aatgacgact tggcctatat cgtctccaat acaacagagg
    18061 caggaatcca attcgtggag actgataatt taacagatac tcccccacag agcctcccgg
    18121 gcaaactgac agccttttta taccgacgtt tcaaattaaa caaagctggc tttaccatca
    18181 ttccttgcga actgattgac agaaacggcg aacgtctaaa agaaatcatt ttacgctacg
    18241 cagaaaaatg ggatttagaa cctgcttttg ttgagtggtt agaaaaagaa aatgttttct
    18301 gttgtagttt agttgatcgc atcgtcccag gttatcctcg cgaagatgca ggtgttttca
    18361 atcaacggca taactatgaa gatcaattga tggtcaaagc agaaccattc atgctttggg
    18421 tgatcgaagg gcctaaagaa ttggagaatc aactaccatt caaacaggct ggcttgaatg
    18481 tcatcatcac agatgatatg actccttatc gtgaacgtaa ggtgcatttg cttaatggac
    18541 cgcatacagc gatggtccct cttgctttgt tggcggaatt ggaaactgta gaagaagtaa
    18601 tgaaagatcc tttattctcc gcatatgttg atcagttatt caatctagaa ttgattccta
    18661 tgctttcttt acctaaagat gaattagcca tttatgctga ccaaatcaaa gaacgtttct
    18721 tgaatccttt tgcccatcac aaattagaag cgatttcatt aaatagtgtt tcaaagttca
    18781 gcacccgtct gcttccagtg ttcaagaagt atatcgaaga acaaaatcag gtaccaccgt
    18841 taatcactgt ttcactagct gcgctacttc ttatgtatcg aggcgatcaa gtaaagccac
    18901 atgatgatga aaagacgata agcgaattta ctgatgcttg gtctgataat ggaacagcta
    18961 ttcctcgatt attgcaaaac gctgcgcttt ggggagaaga tttgtctcaa ataccgaatg
    19021 tgacagatac agttcaagaa atagctgcaa agatcgatca aaaaggtgtc cggtcagtat
    19081 tacaagaaat gaaaggaacg atggtccatg cttgaaaccc tgcaaccaac aatgaaatta
    19141 aatcgacgag acagtgtggt cgtcgcatta acccctattc caaaacaaac acaactgacg
    19201 attgatcagc aaacgatcac cactcttgaa gatatcccgc aaggtcataa gattgcattg
    19261 ggtgatttga aagctggaga caacgtgatc aaatatggct atccaatcgg tcatgtgacg
    19321 acagacgtaa ctgccggtca atggctgcac actcataatg taaaaaccaa tctttctggt
    19381 gaattggatt atcgatatga aaaagatgtc catccaagtc attacctttt tgagaaccga
    19441 acatttcaag gttacttgcg aaaaaatgga aaagtaggga ttcgtaatga tttgttttta
    19501 gtacctaccg ttgggtgtgt gaatggcatt gctgaactga tcgtcaaaca gttcaaggaa
    19561 aaacatccag atctgggaca gtttgaccat attacgattt taaagcatcc ttatggctgt
    19621 tcgcagcttg gaaaagatca tcaaaatact cgagaaattt tagcggacgc agtaaatcat
    19681 cccaatgccg gaggagtttt agtatttgga ttaggttgtg agaacaatac agttcctgaa
    19741 ttcaaaaaaa ttttaggtga atatgatgaa gaacgagtga aatttctagt ggctcaagat
    19801 gtttacgatg agatcgaaca aggtgtggct ctattagaag agcttttaac agcggcggaa
    19861 aatgaccagc gcatatcagt cccgctctct aagttgaatg tcggtttgaa atgcggcggt
    19921 tctgatggtc tatcgggaat aactgctaat cctttacttg gtgcattttc tgactacttg
    19981 attgctcaag gaggcagcac gatcttaaca gaagtgccag aaatgtttgg cgcagaacaa
    20041 gtattgatgg cacgagcgga aaatgaagaa gtttttgact caatcgttga cttgatcaat
    20101 gacttcaaac agtatttctt gtcttacggg gaaccagttt acgaaaaccc atctcctggt
    20161 aataaagcag gtgggatcac tacattagaa gataagtctt taggctgcac gcaaaaatca
    20221 ggaagctctc ctgtcgttga tgtgctacaa tatggcgaaa aaatcagaaa accagggctt
    20281 agtttattgc aagctcccgg gaatgatctt gtagctgcct ctgctcttgc ttcatccgat
    20341 tgccagttgg tcttgtttac cacaggaaga gggactccgt ttggctcata tgtcccaacg
    20401 gtcaaggtat ctacgaatac aactcttttt gaacgcaaag gtcattggat ggattttaat
    20461 gcaggcgtct tattagaaca accaatggaa gtaatcttag aatcgtttgt tcaaaaaatc
    20521 atcgctgtag ctagtggaga agaaacgaaa aatgaacaaa atgacgttcg agaaattgcc
    20581 atttttaaaa acggagtaac actttgattt tcataaaaca gtataaaaag aaagaaggaa
    20641 gatttatgtt tttatctgat gattttttat taaaagacga ttgggcgaaa aagctttacc
    20701 attcctacgc aaaaaacatg cccattattg attatcactg tcacttagat ccaaaagaaa
    20761 tttacgaaaa caacaatttc agcaatctga cagaagcttg gttaggaggc gatcattaca
    20821 aatggcgctt gatgcgagct tgcggcgtgc ctgaagaaaa aatcacagga aatgcttctg
    20881 attttgaaaa atttttagct tggtgtcaaa ccgtaccaaa aattatcgga aatccgcttt
    20941 acagttggtc acatcttgaa ttgaagcgtt tcttcggtat tgacttgttg ataaatgaag
    21001 aaaatgcaga gaaaatttgg acacaagcaa atgaaatttt ggcagctcct gattttcgcc
    21061 gtcgggaaat cgcaaaaaaa tcgaatgtag aagtgatttg tacaacagat gatcccgcag
    21121 acgacttgca ctatcatcag ttgcttgctc atgaagaacc agatttgaag gttctacctt
    21181 cattccgtcc agataaagca ctcaatattg aaaaagaagg atttaatgca tgggtcacac
    21241 tactggaaga agcagctgac caaacgatca ccacctatca agaactgata gaagtcttag
    21301 ggcagcggat agaatacttc catcaacatg gatgtcgtgt ttccgaccac gctttagaca
    21361 tcttacgcta taaagaagcg gatgaaacag tattagaaga gatttttcaa aaagcacgag
    21421 ctaacaaact attatcttca gatgaaatcg atgcttatcg tacagaaaca ctgatacatt
    21481 tgacccactt ctatcattct cataattgga cgatgcagct gcacatccat gcttatcgta
    21541 actgtaatac acaaatgttg gaaaaattag ggcccgatac cggctatgat ggaatgaatg
    21601 atctttcttt gactattcct ctgcaaaaat tattgaaccg tgcagaagaa acggatcagt
    21661 tgccaaagac gatcctttat tcattgaatc cgaatgacta tcctgcgatt ttagctttga
    21721 tgggctgttt ccaaaaagaa cagaatggaa aactgcagtt aggttctgga tggtggtaca
    21781 acgatactcg agctggtatg cgcgatcaat tgacacaatt tgctgatggt agtgctttgg
    21841 ggaattttgt cggcatgctg acagattcaa gaagtttcct gtcctacact agacatgaat
    21901 atttccgtcg tgttctttgt gaatttttag gcgaaatggt catgcgagga gaagcacctg
    21961 aagatgaaca acttttagga agtctagttc aagcgattag ttatacaaat gctaaaaatt
    22021 actttggctt ttttgcctaa ttgattaata gtaacagtaa cgaatcaaaa acaggatagt
    22081 gaaataacgg aatccgttta tccacgtttt agagaagaag acggaccaaa tttgctccgt
    22141 cttcttctct tgctgttctg cttcattgcc tataaacctt tatgaaagaa gagataacat
    22201 gcctactatc catattgccg gtgattctac tgcggcagaa aaagatgaaa acaagcgtcc
    22261 cgagacaggt tggggagaaa aaatctctgc ttatttttca cccgagatcc agatcaataa
    22321 ccaagcaaag aatggacgca gcaccaaatc atttatccag gaaggaaggt tagcccgtct
    22381 agcttcacag tttcagccga atgatttctt atttgtacaa tttggacata acgaccagaa
    22441 aatccaagaa gaaagaggta cgcttccctt cggtagctat ttaaaaaatc tgcagatatt
    22501 ttttgaatgc gcaaaaaaag cgaacgtcca aatggtctta ctgacatctg tgacgcgacg
    22561 agattactta gaaaacgtaa cattgaatcc agatatactt ggagactatc caaaagcgat
    22621 gcgtgcattt gctgagaaac accatattcc tcttttagat gtgttctcaa gatcacaaga
    22681 attgttccag accttctcaa aagaagagac aaaaaaattc tacctgcatc ttatacctga
    22741 tacttgtaaa aattacccag aaggactaaa agacaatacc cactttagtc cagaaggcgc
    22801 tgataaagtt gcccgaataa ttgttgaatt gataaaagaa tcccgtctgc ctcttgctaa
    22861 ttatttacaa aaaggagttt gagatgtttc cattaaattt aggcatccgt gcccacgatt
    22921 tagatgttcg aaatcgtgaa gaaatcgttc aaaaagtatc acagcttcac ttgtcccata
    22981 tccaatttgc accacaaaaa gcatttcctg aacaacttaa attaactgac atgacacttg
    23041 ggagtgctgc ctattttggt gaatacttcc aaaaaaacaa tatcgaactt tccattttag
    23101 gatgctatat caacctatcc agcaaaaatc aagaagttcg tgcacaagca ttggcaactt
    23161 tcaaacagca aattgctctg tgctctgcct atcaagccaa gatgattgct acggaaaccg
    23221 gtagtgtctc tgtcggttac acaaaagaaa attttactga agaagcttat cagatcgcca
    23281 gaaattctat cattgaatta gtcgctcatg cagaaaattt tggcgtaact gttgctgttg
    23341 aagcagggat caatcatcca atctataact atcagttagc taaacgttta atcgatgaag
    23401 tagcttcacc aaatttaaaa atcattttag attgtgcgaa tttgatgcat aaagaaaatc
    23461 atgctgatca ggaaaaagtg atccgaggtg ccttggacaa tttacatggt tatattacag
    23521 cgcttcatct caaagactat atcatggaaa atggaaagat ccgcatggta cctgttggca
    23581 agggctgttt agatttccgc ccggtactgc attatatcaa atatgagcgt cctctaatgt
    23641 acgccactct tgaagctact cctgagattt atgttcctga agcaatagaa cacttgcagt
    23701 ttatttacca agattgttaa ggagaagatt atggaggaac gaatacagaa aagttatcaa
    23761 aaatatcatg caaatgcttt tcagacattg ctgcatcttt atcagggaaa ttattggcgt
    23821 tttgccatgt caggactctt ttttattatc aaacatcttc catcttggtt aatgccgatc
    23881 gctattgcca atgtaatcaa tgcagcttca tcgaaagatt cttctcagtt gcaggccatt
    23941 tatttcaacg cttttttgat ggcttgtctg attggtcaaa atatattgac gaattatttt
    24001 catgtaaaat accatagcac ttcgatccgt caagtagaag ctggactaag agcgaccctt
    24061 atcaagaaaa tccaagaact atctattcct tatcaaaaag aattgcaatc tggacgaatc
    24121 cagtcaaaga tcatccgtga tgtagaagct atccaaacgt tgtcctctca agtatttgtc
    24181 agcggattaa atatcgctgt caatttattc gtagctttag gtatcacgct ttataaaagt
    24241 cctgttgttt tcatgttttt cttattgact gttcctctcg ccgcattcgt tactctcttt
    24301 ttccgtaaaa ggatcagaca gtccaactca gacttcaggc aagaaatgga agagacttct
    24361 gcaaaagtga tcgaagtgat cgaattagca ccgattgctc gagctcatgg gttagaaaaa
    24421 cacgaaacaa ctcgcttaaa taagcatttt actcaaatct atgaaaaagg attccactta
    24481 gatatcctgc aatctgtttt cggatcagtc agctgggcta gttttcagat atttcagctg
    24541 atttgtttga ttttcactgg gatattagct ttacaagggc gcatccaacc cggcgatgtc
    24601 gtgatgtatc agacgtattt cacgacgatc gtcaattccg tttctggaat gatcacgctg
    24661 attcctacga tttcaaaagg catggaatct atcacttcta ttggcgaact gttgattgct
    24721 ggcgatgtag aaacttatca cggtaagaaa aaaatccaac atttggatgg aaattttcgt
    24781 tttgaggatg tttcttttct ttatccggac tcactcaaac cgattatcga tcatttttct
    24841 ctagaggtga aagctggaga aacaattgct tttgttgggc catctggctc tggaaaatct
    24901 acactcctta acttattgat tggatttatc cagccgactt caggaaaaat ttttctagat
    24961 gatcagcctt tggatgaact ggatatgcga agtgtgcgaa attatttagc cgttgttcct
    25021 caaaccactt tgttgttctc cgcttcgatc aaagagaaca tcacttatgg cttgaaaaac
    25081 gtatcaaaag aacgtttaga tgaagtcatc gaagctgcgc aactttcttc actgattgac
    25141 cagcttcctg atggtctgga taccttagta ggcgaacatg gcaacaagct gtctggtggc
    25201 caaaagcaac gtatctccat tgcacgagcg ttgatacgtc agccaaaaat cattttgcta
    25261 gatgaagcaa cctcagcttt agataaccag tcagagaaaa aaatccagca agccctgaat
    25321 tacctgacag aaacacccac tacttttatt gtggctcatc ggctttcaac gatcaaagag
    25381 gcagataaaa tcgttgtgat cgatgaagga aaaatcgttg aaataggtac ttacgaaaaa
    25441 ctgttagctc aaaaaggcta tttctaccgc ttgaaaaatg aatgacaaaa aataggagct
    25501 atgacaagac ttttgtcgta gctcctattt ctgaataaac ggtgttctcc tgccaactcc
    25561 tcggtcacaa gtcacaatag tcatcaaaca tgagaagctg ttttttgaaa ttgttccttg
    25621 taactcagag ttaaacggta ggctcacgac ctcttggtaa atggtgttca agagacagcc
    25681 tatcggcaat aagaagcaat tttgtcatcg ctcaaggccg aacgtctctt tcacaccctc
    25741 tgttgtttca taaaatagtt ttattcgctg taaaggtcgc gaactggtgt catgatgata
    25801 cggttcaagt cattgatgat tgtactgaag ccttgctctt tttgcatcaa gtcattgatt
    25861 acttcttctg attggatttt cacagccatt tcttgtgctt tcattgcatc ttcgtcgctg
    25921 aactcttgtc cactcatttg tttttcttgt aattcgattt gcatcgcttg gaagtctttg
    25981 aacagtttgt gtgcttcttc gtttgcttgt actttctcgt acgcttcttt caacgctttg
    26041 aattctggta attcacggat ctcacgttcg atttgattcg cgctatcgta gatgtttgac
    26101 atgttatgcc ctcttttctt ttagtagtta ctctctattg taacatgggg ttattcgttt
    26161 tcaaaggggc tactctcctt gatatttccg ccagaggtct ttagccgctt caccaattcc
    26221 ctgaacgcct tctttcacca tatcgccgaa gtttttcagc ccttcttttg cttgttcacc
    26281 atattgattc aagttatctt gccaatttcc agactctcca ttagaatttc ctgtgtttcc
    26341 aacttgatct gcagctacca cttgtcctcc tgtggcatag gcatctgcta cactaaatcc
    26401 tgtttttgaa gtatacggaa ggatgccttc tgccgctgat ttgaagactt tcccgacaac
    26461 atttccactt gtcccttcca aatagtgcag cttgctggat tcttcgtaac ccatccatgt
    26521 cgaaatcact acgtctggcg tgtaaccgat catccattgg tcatttgctt ttgtggcatc
    26581 gaaattagtt tctgtggttc ctgttttccc tgctatcgta taacctgcag gattcgcagc
    26641 agccgctgtt ccatttgaaa atgtaccaag aagcatgctg gtcatttcat cggctgtttc
    26701 ctttgatatg acctgttttg gttttttgtt tgtgttatcg acgatcaccg ctccagtcga
    26761 atcaacgatt ttcgtgataa gatgcggcgt atacatctga ccatcattgg caaatgcact
    26821 gtaagcaccc gccatgatca atggagatac tcctttttcc agtccaccaa gggctaagcc
    26881 ataatactta tcagattttg ctaaaggcag gccgaattct tctgctttat catatccttt
    26941 ttgtaaaccg atctcatgca aaagccatac agtaggcaaa ttcaaacttt gtgcaacagc
    27001 ttgatacatc ggtacttctc cttggtatgt cccgtcaaaa ttatgcgcat cgtaataact
    27061 ttgcggttcg tctttcaaga tactggaagg cttgtatcca gcttccagtg ctggtgtata
    27121 tacgcttaat ggtttgatcg ttgatcctgg tgaacgacgc atctgtgtag caaagctgaa
    27181 tcctctaaag acatgatctc ctcttcgtcc tactagtgct tgtacaccac ctgtattcgg
    27241 atctaaagct actgaagcag attggaccat ggcaccatct gaagcatttg ctgggaataa
    27301 tgcactgttt tgataagtag cttctaattg cttttgataa tcctgatcta aagctgtata
    27361 gattttatag cctttattca tgacatcttc ttcatcaaga ccatactcgt taactgcttc
    27421 atcgatcact gcatcaaaat aataaggata acgataacca gaatctgaat tatcgtatgt
    27481 gtcattaaga tacgcgctga tatctgtctg gctttctgct tgttcttgtt cttttgtgat
    27541 ttttccgttt tcttccatta cacttagtac tgtgtttttc cgatcattcg cattttttgg
    27601 gtgatcgatc ggattataaa taccaggacc tttcagcatt cctgccaaag tagcagcctc
    27661 agacagcgta acattcgttg catctactcc aaaatacttt ctggaagcgt cttggacacc
    27721 ccataccccg ttgccaaaat aggcattgtt aagatacatg gtcaagattt cttctttgct
    27781 gtacttcttt tcgatctcga ttgccaaaaa taattccttc gcttttcgat caaacgtttg
    27841 gtctaatgtc agatacgcat ttttggcaag ctgctgagtg atcgtacttc ctccgccccc
    27901 tgatgttccg ccagacaaga ccattcggac tgctgcacgg gcaatccctt tgatatcaaa
    27961 cccgtgatgc tcgtaaaaat tcctatcttc tgtcgagatt actgcatctt ggatataggg
    28021 agaaatcgcg tttaattcta catatgttcc tttttgggcg tacagtgtgc cagcttcttc
    28081 gtttttctta tcataaatga ctgtcgattg gcttaatcct gattttaacg tttcgacatt
    28141 tgcttgtttt gctaaaataa agaggtagat acttgtcact aagacacaga tcaaaccaat
    28201 cagcaaaatc aatttgttca catgatattt tttccagatg cgctttcgcc atcgatggaa
    28261 ttgtccgaga tatggtctga tccaacgcca aaaactgatc aaagcagctt ttatcttttc
    28321 taaaatgctt tgaaaatcca tttgcttctt ctcctgtcta tttgattcaa tcgtcttatt
    28381 ttaccatagt gaaagagtgt tttcttcctg cttaagttgt agttcaatat gtttttttac
    28441 gttagtgcaa gtagagtaac ataagaatga acagaaaaat gtaagttttt tcttaaatat
    28501 gttttaaaaa tggtacaatt agtctgcgat tgcactgaaa ggagtacact tgtgaaaatc
    28561 acgatcactt tacctatgaa ccatcctgaa acaacaattc gcgacttgct tgaaaaggaa
    28621 tggttagttc ctagaaaggt tcgacacttt ttgcgtatac gtaaaaacgt ttggatcaat
    28681 caacaacctg ctttgttcca tcatgaagta aagggcaatg accagatcac attgttattc
    28741 gaagaaacag attataatta tcaagaagtt caattaggag atgcgaagca catccgtgtt
    28801 ttatatgaag atgagcattt gattatagtg gataagcctg caggaatgaa gactcatcca
    28861 aatgaaccga cagaaaacag taccttactc aatcatttag ctgcttatct cgacactaaa
    28921 cagcaacgac cttacgttgt ccaccgcttg gacaaagaaa caagcggtgc cgttttattt
    28981 gcaaaaaatc catttgtctt gccgatactt ggaagaatgt tggaacaaaa aatgatctat
    29041 cggagatatc aagccatcgt atggggagaa attgccaaag atatgaccat caccaataaa
    29101 attggacgag atcgccatga tcggagaaaa agggtcgtag atgataaaaa tggtcagagc
    29161 gccttgacac atgtcactgt caacaaagta ctttctgaac aaacacaagt ctattgtgtt
    29221 ttggaaaccg gacgtacaca ccagatcaga gttcatttag ctcataaggg acatccgatc
    29281 gttggagacc ctctctacca aaatagaaca gctgaacgac tgatgctcca tgcattagaa
    29341 ctgcacatga tccatccatt tactcaagaa aaaatagtcg gtaaagcttt acctggatta
    29401 tgggaataga aataaaaaag aacagagtct tatcagcaga taatgataag actctgttc
//
LOCUS       DACGLE010000028        29152 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0534
            SAMEA3304449-rid9560453.denovo.029, whole genome shotgun sequence.
ACCESSION   DACGLE010000028 DACGLE010000000
VERSION     DACGLE010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304449
            Sequence Read Archive: ERR985064
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 29152)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29152)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702225.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702225.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 20:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,045
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 27 of 67
            Pseudo Genes (incomplete)         :: 25 of 67
            Pseudo Genes (internal stop)      :: 19 of 67
            Pseudo Genes (multiple problems)  :: 4 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29152
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304449-rid9560453.denovo.029"
                     /strain="VREN0534"
                     /db_xref="taxon:1352"
     gene            complement(174..1754)
                     /locus_tag="IXT20_001372"
     CDS             complement(174..1754)
                     /locus_tag="IXT20_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HAQ4859827.1"
                     /translation="MNNPELKQQVDNRRTFAIISHPDAGKTTITEQLLLFGGAIRQAG
                     TVKGKKTGNFAKSDWMEIEKQRGISVTSSVMQFDYQGKRVNILDTPGHEDFSEDTYRT
                     LMAVDSAVMVIDSAKGIEAQTKKLFQVVKKRGIPIFTFINKLDRDGREPLELLEELEE
                     LLDIESYPMNWPIGMGKGLEGLYDIYHNRVEFYRPENYQDERLVELDEDGLLPENHPL
                     TENSLYEQVLEEVELIKEAGDAFNQEKIARGDQTPVFFGSALTNFGVQTFLETFVELA
                     PAPYGHKTQDDQMVSPYEEEFSGFVFKIQANMNPAHRDRIAFVRICSGTFERGMDVWL
                     ERTNKKLKLSNVTQFMADSRENVEKAVAGDIIGVYDTGNYQIGDTLFEGKLKVAYEEL
                     PSFTPELFMKVTAKNVMKQKSFHKGIYQLVQEGAIQLYKTYLTEEYIIGAVGQLQFEV
                     FQYRMLNEYNAEVIMSPMGHKIARWIDPEDLDEKMSSSRNILARDRFDQPLFLFENQF
                     AERWFADKYPNVKLKSLM"
     gene            complement(2155..3531)
                     /locus_tag="IXT20_001373"
     CDS             complement(2155..3531)
                     /locus_tag="IXT20_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010767236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HAQ4859828.1"
                     /translation="MNADPESQSLLAQILLLIVLTLINAFLAASEIAVVSINKNRIEQ
                     KADEGDVKSQKLLKILQNPNNFLSTIQVGITLVNILSGASLANTLSERLAPVLGGGTA
                     AKNIANIIVLAILTYVSIVFGELYPKRIALNKSEEVAKFTSGLIRLIGVVAKPFVWLL
                     SASTSLLARITPMTFDDEDSKMTRDEMRYMLENEGVLNNEELEMLQGVFSLDTKVARE
                     VMVPRTDAFMIDINDTVEENVNEVLSENYSRIPVYNEDKDKVVGILHTKNLLKAAHKF
                     GFDNLDIKKIMQEPLFVPETVFIDDLLYEMKKTQNQMAILLDEYGGVVGLATLEDLLE
                     EIVGEIDDESDEVENLYEKIDEHEYIIQGRMLIDEFNEAFDSDLHMSDVDTMAGYLIT
                     ALGMIPDEGEKLSFDVDNITLVSEEMEGSRILKIRVIFHDPEETEAEPEEERRYFRKE
                     FEDDEPRR"
     gene            complement(3659..4777)
                     /locus_tag="IXT20_001374"
     CDS             complement(3659..4777)
                     /locus_tag="IXT20_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAQ4859829.1"
                     /translation="MFEKLRQSKLMFWSLELLIIAALIMISSEINFIFEPIGTFFSTL
                     FAPVLIAGFLYYLLNPIVKLLMKAKIKRIWAVAIVLLLLVAAIIWILLSVIPSLVQQI
                     SSLASNMPDFIKQVESWLKEVAELPLFKEVDINKYFEQLDISYGTIIQQFLSGVSNSL
                     GSIVGTIASATVVIITAPFILFYMLKDGEKLVPNIQQFFPEKRRNQIVELLGQLNKTL
                     ANYISGQAIECLFVGTFTFLGYMVIGVDYAFLFGVIAGLTNLIPYLGPYLGLAPAFLV
                     TVFNDPVKALLCCVVVLIVQQLDGNIIYPNVIGKSLKIHPLTIIIVLLVAGNIAGLLG
                     IFLGVPFYAICKTIISYIIKIVKEDKQNENKKKIATTS"
     gene            4919..5353
                     /locus_tag="IXT20_001375"
     CDS             4919..5353
                     /locus_tag="IXT20_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ4859830.1"
                     /translation="MKVVQTKDTMSNIYLDAVRIRHQVFVVEQGVPLSREIDKDEAHC
                     IHFVLYSDKKEPQGTVRLLPLENGKMKLQRMAILSEYRHQGLGKILIEEAENFAKNQG
                     YNTILLGAQSTAETFYEKLGYTAYGDPFEDAGMPHIYMKKTL"
     gene            complement(5431..5964)
                     /locus_tag="IXT20_001376"
     CDS             complement(5431..5964)
                     /locus_tag="IXT20_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HAQ4859831.1"
                     /translation="MGVPKEGEFVTIQSYKHDGKLHRTWRDTMVLKTSEHSMIGVNDH
                     TLVTESDGRRWVTREPAIVYFHKKYWFNVIAMIREKGVSYYCNLASPFLLDDEALKYI
                     DYDLDIKVFPDGEKRLLDVDEYEMHSKMMNYPNDIDFILKENVKILVDWINNGDGPFS
                     EGYIDIWYNRYKQLSRK"
     gene            complement(6058..7236)
                     /gene="mutY"
                     /locus_tag="IXT20_001377"
     CDS             complement(6058..7236)
                     /gene="mutY"
                     /locus_tag="IXT20_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HAQ4859832.1"
                     /translation="MNKEWEKWTDKETKEFQDQFIQWYEQEKRNLPWRYNRDPYRIWI
                     SEIMLQQTRVDTVIDYFYRFMEWFPTIEELANAPEEKLLKAWEGLGYYSRARNIQAAA
                     KQIMSEFDGEMPQTPEEISSLKGIGPYTTGAIASIAFGLPEPAVDGNVMRVVSRLFCI
                     EADIAKASSRKIFDEAMRKIIDEKHPGEFNQAMMDLGSAICTPTSPKCETCPIQAFCL
                     ANKRGIQTSFPVKTKKAKPKDVYYISAALQNHSGAYYFEERDSQKLLANMWTFPMMEV
                     TQEEYERLKKEWETKPEIDLFDDLVAEEEQNLPFEKQELFVWQTRHLGEVTHIFSHLK
                     WHVLLFYGRATEGAEQEFTENKTSKWLKPDAFDSVVFPKVQMKLVDQLEKNRNNRNPF
                     "
     gene            complement(7229..8026)
                     /gene="recX"
                     /locus_tag="IXT20_001378"
     CDS             complement(7229..8026)
                     /gene="recX"
                     /locus_tag="IXT20_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HAQ4859833.1"
                     /translation="MLTVTRISKGKGPFYRVDLSEGEALQVSEDVLVRYRLLKGRELD
                     EETIQEIKKSSGADFGFHLAMNYLSYQLRSEKEIRTYLKEKEILQEDRNKIVARLKEL
                     DLVDDLVYGESYVRTNMRLSDKGPKKLAQQMQQKGLKPEIIEQALSQYTFEDQVQNAH
                     QTAEKAFGKNRGKSQKELLRKIQQTLMTKGFSQEVIQQAMADLPQEADQEIEYDYLVK
                     QGDKLWRRNLRFEPKKRNLKVKQSLYQKGFDLDMIQRFITEKEEESE"
     gene            8133..9515
                     /gene="rlmD"
                     /locus_tag="IXT20_001379"
     CDS             8133..9515
                     /gene="rlmD"
                     /locus_tag="IXT20_001379"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HAQ4859834.1"
                     /translation="MAETIVKTGQTVPLKIKRLGINGEGIGYFKRLIIFVPYALPKEE
                     VLVKITKATPRYAEGELIKVKKTSKDRVVAPCPVYYECGGCQLQHLAYQAQLDFKKDL
                     LLQALEKFKPAGFRSYQLLPTIGMDEPWHYRNKAQFQLRKNKQTQKIEAGLYEANSHQ
                     LVPITDCLVQEPRTQKVMNTVVQLLNKFDAPIYDERTNSGIFRTIMVRIGVKTGELQV
                     VFITRSKKFPQKNAMIREINERLPEVVSIMQNVQAQKTSLVMGDETIHLWGKEAIEEQ
                     INEVTFDLSPRAFFQLNPQQTELLYGEGIKALDVQPNETIVDAYCGVGTIGLSVAKQA
                     KEVRGMDTVPQAIADAKKNAERLGYHNTRYEVGTAEQVLPKWLNEGFQPDGIIVDPPR
                     TGLDNALIQSLLKYPPKKLVYISCNVSTLARDLVNLSKKFKIEYLQSVDMFPQTARCE
                     VVVKMTRLAD"
     gene            complement(9689..10267)
                     /locus_tag="IXT20_001380"
     CDS             complement(9689..10267)
                     /locus_tag="IXT20_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitin-binding protein"
                     /protein_id="HAQ4859835.1"
                     /translation="MKKISTLLFIGLVSLGLFGFSIPGFAHGYITSPGSRAYLGTSAA
                     GNLNQNVGRAQWEPQSIEATKNTFIDGKIASAGVSGFEPLDEQTSNRWHKNMVNPGTL
                     NITWNLTAQHRTSTWDYYITKPTWNPNQPLKFSDFELITKIDDKATVPPKTVNQTITL
                     PQDRKGYNVILAVWNISDTTNAFYQVIDVNIQ"
     gene            10589..11059
                     /locus_tag="IXT20_001381"
     CDS             10589..11059
                     /locus_tag="IXT20_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan-rich sensory protein"
                     /protein_id="HAQ4859836.1"
                     /translation="MKMIKDIRFWVCVIGIVILGFLSGLLSGNPGEYYYSLQLPPFAP
                     PSWIFGPMWTLLYILMGISLYLLLNHNNKKQRNNLVGLFVIQFIFNFIWSALFFNLRN
                     IFIAAIDITLLVIFLSVLMYQLWLHHRLAMWLMIPYYLWVLFATLLNYSIYFLN"
     gene            complement(11167..12393)
                     /locus_tag="IXT20_001382"
     CDS             complement(11167..12393)
                     /locus_tag="IXT20_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytR family transcriptional regulator"
                     /protein_id="HAQ4859837.1"
                     /translation="MKRWQKVLLTLLGVFVLLIAGLSAYGIRFMGEASQTVNKISKNS
                     NRISSKRSERVSIDDKEPFSVLLLGLDTGGLGRTEQGRSDTMMVVTVNPKQKKSTIIS
                     LDRDIYTNIVGYGTVDKLNHAYAFGGVEMAMDSIEQLLDIPIDHYVTINLDGMEDLIN
                     AVGGIKVNNKIDFTLDGVHVPVGEQTLNGEKGLAYSRMRHEDPEGDIGRQRRQREVVT
                     KIVNKVLSLDGVSNYRKILKAVENNVTTDLDWDDMLDVATNYTPAFETIKQDQLQGEG
                     QMINDIYYQILGKNDLLSIQNELKKQLNIKTSDTLPNLKNENASMMFFDDSEDGDSTN
                     GDGSNSEGYTDYSSYSENTYDQYQDPNTYYQQTEDTSQYYTPSQDTQSYDPYAGQEQN
                     GYGYDQNNEYGTTSGY"
     gene            complement(13147..13347)
                     /locus_tag="IXT20_001383"
     CDS             complement(13147..13347)
                     /locus_tag="IXT20_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAQ4859838.1"
                     /translation="MEHGTVKWFNNEKGFGFITVDGGDDVFVHFSAIQGDGFKSLEEG
                     QEVEFTIVEGARGPQAAEVTKL"
     gene            complement(13481..14089)
                     /locus_tag="IXT20_001384"
     CDS             complement(13481..14089)
                     /locus_tag="IXT20_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HAQ4859839.1"
                     /translation="MTYTLPDLPYAYDALEPYIDEETMHLHHDKHHNTYVTNLNAAIE
                     KYPELGEKTIEELLSDMDAIPTDIKTAVRNNGGGHANHSFFWEIMAPNAGGEPTGEIK
                     EAINEAFGDFSSFKEEFKKAAAGRFGSGWAWLVMENGKLAITSTANQDSPLMEGKTPI
                     LGLDVWEHAYYLKYKNVRPDYIAAFWNVINWDEVNKRFEAAK"
     gene            complement(14174..14644)
                     /locus_tag="IXT20_001385"
     CDS             complement(14174..14644)
                     /locus_tag="IXT20_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HAQ4859840.1"
                     /translation="MKQYRQLKGYLEAKGYWEILVYLFFGGLATIVNFVSFALARQYF
                     DLSMALSNSISWFCSVLFAFVTNKLWVFHSKSPNFTHALIECGKFFFYRILSYGLDMG
                     AMVLLINVMNSNEYVAKIITQIIVILANYIFSKLFIFKETEVFEEEVGQSEKDD"
     gene            complement(14692..15606)
                     /locus_tag="IXT20_001386"
     CDS             complement(14692..15606)
                     /locus_tag="IXT20_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HAQ4859841.1"
                     /translation="MEEGIVPTQPQRSSKKLFGVVVLNAVITLSEFIGGIMSGSLSLV
                     SDAFHNLSDTLAIIFSYGAQKMARKEANKKRTYGYQRLEILSAFINSFILIILSLFLT
                     VEAFKRFTSPEKINSHLMLTVAVIGLLANLFSVLLLRQEADESLNIKSSYLHLLSDTL
                     SSVSVIIGAILIRFFDIYWIDPVITLIISIYILIEAIMVIKKAAAILIQSAPAIDYEK
                     MEQEIEAIDGVKNVHHVHIWQYSEKIIIFDGHIDFEDQLLSEIEKIYPRIISLLKLKY
                     GITHVTIQAETKAKDQKKLIFLNKDESY"
     gene            15752..16555
                     /locus_tag="IXT20_001387"
     CDS             15752..16555
                     /locus_tag="IXT20_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1189 domain-containing protein"
                     /protein_id="HAQ4859842.1"
                     /translation="MTTKQLIISSFIRFKDLKETRNVPFWKAIVYLLVLSIIMALPIS
                     FQVFQVLNNIQLDGQKIATRIPDFTIQNGQLQTEEKEGFIYQTNSIIFTFDPEGKRTE
                     QDISTDLMGNFVSVGMLKNKLIIALPNTGTTSALLNNNQLELPYTNESLKNLTGKELR
                     SFLSKASIPLWIKVLTFIFSIYPSFLNLLITLLFTNIAAFLYARFRLTKATFLDCLKT
                     LIYSISLPTIIATILMVFLPSFDTSAFIAFAGIFIFAQAVKGWAKISIN"
     gene            16733..17680
                     /locus_tag="IXT20_001388"
     CDS             16733..17680
                     /locus_tag="IXT20_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HAQ4859843.1"
                     /translation="MSERIIYADRPFRDEFIEEIHTIAPGYIFKTELQPEDLQNVEIS
                     LGWNSDYQEKLLASSTLKWVQSISAGVDQLPLETFSKKHILLSNGSGIHSESITEHVL
                     GIILGYSRGLFQAQKAQSDMKWLGSSVHYQSIQGKKILIIGTGHIGKMIAKKCKALDM
                     NCYGINTSGHPAEGMEKTFSLNQLKEVLPEVDFVVNILPLTEETTGLFDQHMFEHFDP
                     KSVFINVGRGASVKTQDLVQALNNQQLSFAALDVFEEEPLPQDHPLWKMDNVLITSHI
                     AGLTPDFQKKLMAIFLTNLKSYFATHELQTNQVKLSAGY"
     gene            complement(17748..18041)
                     /locus_tag="IXT20_001389"
     CDS             complement(17748..18041)
                     /locus_tag="IXT20_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis protein"
                     /protein_id="HAQ4859844.1"
                     /translation="MELTVTPKAQKWFDSEIDLPETYGIRFFGKVYGKTEVHDGFSIG
                     MSVEQPEHPVKKVEMDGTLFFIEEADEWFFKGYDLLVDYDPNLEEPTYHFKKQ"
     gene            complement(18069..18734)
                     /locus_tag="IXT20_001390"
     CDS             complement(18069..18734)
                     /locus_tag="IXT20_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HAQ4859845.1"
                     /translation="MKQNFAIVGLGRFGGSICRTLIEAGQEVLAIDSNEDRVNEYMNI
                     ATHAVVANAQDEMTLRSLGIRNFDHVIVAIGEDIQASILVTLMVKEMGVPNILAKAQN
                     EYHARVLEKIGADRVVHPERDMGIRIAHNLVSKNILDYLELSDKYSLAEIRVSNPKFF
                     NKTLAELNFRQRFDLTVVAIRRVDQTVIASPAADEYVRENDNLLVIGETGDVDILDDK
                     MNQ"
     gene            complement(18874..19506)
                     /locus_tag="IXT20_001391"
     CDS             complement(18874..19506)
                     /locus_tag="IXT20_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ4859846.1"
                     /translation="MIKVLLVDDHEMVRLGVSSYLSIQEDIEVIGEAENGRQGYEKAM
                     ALRPDVILMDLVMEEMDGIESTKAILKDCPKAKIIIVTSFIDDEKVYPAIEAGAAGYL
                     LKTSTAHEIADAIRATQRGERVLEPEVTTKMMEKMSRRNDPVLHEELTNRENEILMLI
                     SEGKSNQEIADELFITLKTVKTHVSNILAKLEVEDRTQAAIYAFKHGLVK"
     gene            complement(19513..20580)
                     /locus_tag="IXT20_001392"
     CDS             complement(19513..20580)
                     /locus_tag="IXT20_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAQ4859847.1"
                     /translation="MMGRISKAMLAVYSGIAAFLIILFSLFTYFYASNQSHWWGELLR
                     ARLLYVPLIFHLMAISLGVGLIVFLLLSLIQKAKYGKIEEKLRALSSGNYESKLLHQP
                     IPSASDDLYIKDIDMEIAKIKEKMIEVSSELQIMTSRPQYVDGQTKEEILELERHRLA
                     RELHDSVSQQLFAAMMMMSALTEQAEKSETPEMFRKQLKMVAEIINASQSEMRALLLH
                     LRPVNLEEKSLKQGIEQLLKELQNKIQISLKWDVEDVKLTSSIEDHLFRIVQELLSNT
                     LRHAKANELEVYLHKIDNNLLLRIIDDGTGFNMNETKTGSYGLNNIKERVAGIGGTVK
                     IISFKGQGTSVEIKVPLMKEA"
     gene            complement(20577..21308)
                     /locus_tag="IXT20_001393"
     CDS             complement(20577..21308)
                     /locus_tag="IXT20_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-active antibiotics response protein"
                     /protein_id="HAQ4859848.1"
                     /translation="MNSSWRFFVVVEALLLLFAVWQIVNNTGLLLLVIFGIFNIYLAM
                     RKYPRTKFQNFQLILGSLVIFFSLVNSPALWMMVVLAVLFIGLKGVEISGIDLTKNAF
                     WRKKQIMMVQTEQLKTHNNERKRQQLFGNQRIGNDVYEWDDINIAIISGDTIIDLGNT
                     LLPKDDNIVIVRKGIGRTRILVPLGVAIRLEHATLVGNVLFEEEQFSLKNEQIKICSS
                     DYDENPRRLKIITNTLVGDVEVIRI"
     gene            complement(21476..21955)
                     /gene="greA"
                     /locus_tag="IXT20_001394"
     CDS             complement(21476..21955)
                     /gene="greA"
                     /locus_tag="IXT20_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HAQ4859849.1"
                     /translation="MVEKVYPMTLEGKEKLEKELEELKTVKRGEIIERIKIARGFGDL
                     SENSEYESAKDEQAFVEGRITTIENMIRFAQIIDNDGVDADEVSIGKTVTFIELPDGD
                     EEEYTIVGSAEADPFSGKISNDSPIAQALIGKRKNDEVTIATPGGDMLVKIVKVEQA"
     gene            complement(22091..23266)
                     /gene="mltG"
                     /locus_tag="IXT20_001395"
     CDS             complement(22091..23266)
                     /gene="mltG"
                     /locus_tag="IXT20_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HAQ4859850.1"
                     /translation="MSGNKQHPSKDKKEASKAATADIIDKRRNLRKKEDKIVRKIILV
                     IALTLLIIGGFLGFTVYRYVDSGLKPLDKSDDQLVQVEIPNGSSNKQIGEILEKDNII
                     KSGIVFNYYTKFKNLTGFQAGYYQLAPNMTLDEIGKQLQEGGTSEPTKVADGKIAIPE
                     GYDIDQIAERVAKVTGKDKKEFLDLVNDETFFNELYQKFPELLESASKAENTKYRLEG
                     YLFPATYDYYTSTSLKDLVIEMVNKTNTVMQNYYSAIKQKNLTVQEVLTLASLVEKEG
                     VKENDRKNIAQVFFNRIKANMPLQSDISVLYALGEHKELVTYEDTAVDSPYNLYTNTG
                     YGPGPFDNPSEKAIKAVLEPAENDYYYFVADTSTGNVYFAKTYEEHMELVQKYVNNS"
     gene            23431..24591
                     /locus_tag="IXT20_001396"
     CDS             23431..24591
                     /locus_tag="IXT20_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HAQ4859851.1"
                     /translation="MAKGKHQKKQQISLSFLAVLLLAVSFFSTGIITGWQLTKQPQPQ
                     IYKTEPAQLASNLTSNEQMLASKIDQLLQKSDYIGSIYVRKNNRVILEKGYGYANLRI
                     KESNSPSLYYQIGSIQKAMTALLILKQIQSGKITFDTKLSEFYPQIPGSQQISIKNML
                     YMRSGLHRTASPKKPMSDSEIIQFALHHLKFTGYDTYHYEPLNYTLLTGVLIKLMNQP
                     YETLLKKEIIRPLHLEHTDFYENVKNSPQHAVSYQMSATDDYSKALVEPETDIRNELG
                     TGNISMSVYDLNKFFTSVLSGDIIPKELLFSLWQNNGDKHPYSGGVYSGNDYILAQGN
                     INRFHAVAAMKKDTKDAIVMESNIQSDKNIKLPATDLRNQIYELMEGVNLKS"
     gene            complement(24631..25578)
                     /locus_tag="IXT20_001397"
     CDS             complement(24631..25578)
                     /locus_tag="IXT20_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HAQ4859852.1"
                     /translation="MDTILITGGAGFIGSTLANYLGKENKIVVVDDLSMGKKENLDME
                     KQVTFIEGDVSDPQLMERIMKQYQFAYIFHLAAVASVADSVERPLETHRVNFDSALLL
                     LELVRKYQSGLKRLVFSSSAAVYGDEPTLPKKEESVIRPLTPYAIDKFASEQYVLDYC
                     HLYDVPTSAVRFFNVYGPNQNPNSPYSGVISILVDRYKKQLAGEKTEFTLFGDGSQSR
                     DFVYIEDVIQALLLVAKEEKALGQQFNVGTGKSTTLLELIHSIDQILGTELALKYEAE
                     RSGDIRDSLADISKIRSLGYQPKFDILNGMERYLKTEID"
     gene            complement(25711..27102)
                     /locus_tag="IXT20_001398"
     CDS             complement(25711..27102)
                     /locus_tag="IXT20_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymerase"
                     /protein_id="HAQ4859853.1"
                     /translation="MLNEKFLKYVKGWYFIYFGFILMLRAIGPYLLLPTRYDTLLFWP
                     AGIAGVVLIIIDAIIAFREKNLKKYDWLLIAFIGAMLVSSLMNLKYGYSENIKLIMWQ
                     LIFFFMIYQFGKDYSSNFFFKVFTWILSLVWFLANLVSLYWFVIQYGIKIPIKYKYYP
                     VRLGWLGNRLFGVYTDPNFGAVISLIVIIIAIYYLVTYKHLNLLFKIFFAINIFIQFS
                     FIALSGSRTTLVCLLIATFVFSFFTSYSSRFLKTNTLKRWLTALVVGLLSIIAMYYVT
                     DITAQAWAHVPHITEIKKDISDSSGTTSSNKVDLKRPDTDGGDVSNLRFGLWKSAIEI
                     FKSKPISGGSPGYYIEYAHDKLPYTLMGKDTLTAHNFIFLVMAATGGLGLLTLLTFLF
                     IQCFKTLRYCFTQPNLVKDKHFFAILMILTIAVSALFITELILVSTIGSFIFWGYLGS
                     IQKTISKEEGVTN"
     gene            27228..27638
                     /gene="tagD"
                     /locus_tag="IXT20_001399"
     CDS             27228..27638
                     /gene="tagD"
                     /locus_tag="IXT20_001399"
                     /EC_number="2.7.7.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate cytidylyltransferase"
                     /protein_id="HAQ4859854.1"
                     /translation="MKRVITYGTFDLLHYGHINLLKRAKQYGDYLIVALSTDEFNWNA
                     KQKKCYFSYEKRKQLLEAIRYVDLVIPEENWEQKVKDIKEYHADYFVMGDDWAGQFDE
                     LEQLTDAQVIYLPRTPEISTTQIKNELNSLNSDK"
     gene            27818..29107
                     /locus_tag="IXT20_001400"
                     /pseudo
     CDS             27818..29107
                     /locus_tag="IXT20_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351973.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
BASE COUNT         9004 a         5825 c         4661 g         9662 t
ORIGIN      
        1 taactagcac cacgggtatt tttatatttt tttgattaga catttctatg attttaatat
       61 agtaaaaaga gccgtcacgg aaaatccgtc acggctctaa acaaaatatg agaggctgtt
      121 tttgtttggc tattcttatt acttgatgca gggcagcttg tgtcaagact tctttacatc
      181 aaacttttta attttacatt aggatattta tccgcaaacc agcgttctgc aaattggttc
      241 tcgaataaga acaatggttg gtcgaaacgg tcacgagcca agatattgcg acttgaactc
      301 atcttctcat ccaaatcttc tggatcgatc cagcgagcga ttttgtgtcc cattggagac
      361 atgattactt cggcgttata ctcatttaac atacgatatt ggaatacttc gaattgtagc
      421 tgaccaacag ctccaatgat atattcttcc gttagatatg ttttataaag ctgaatcgct
      481 ccttcttgaa ctaattgata gattcctttg tggaaggatt tttgtttcat gacatttttt
      541 gcagtcactt tcataaacag ctctggagta aatgaaggga gttcttcata agcaactttt
      601 agttttcctt cgaacaatgt atccccaatc tgataattgc ctgtatcata tacaccgatg
      661 atatcgcctg ctactgcttt ttcgacgttt tctctcgaat ctgccataaa ttgtgtgaca
      721 ttgcttaatt tcagtttttt atttgtacgt tccaaccaga catccattcc tcgttcgaat
      781 gttccagaac agattcggac aaaagcgatt cggtcacgat gggctggatt catatttgct
      841 tggatcttga atacgaaacc tgaaaattct tcctcataag gacttaccat ctgatcatct
      901 tgtgttttgt gtccataagg agctggtgcc aattcaacga aagtctctaa aaaagtctgt
      961 acaccgaagt tagtcaatgc agaaccaaaa aatactggtg tctgatcgcc acgagcaatt
     1021 ttttcttgat taaatgcatc tccagcctct ttgattagtt ctacttcttc taagacttgt
     1081 tcatataagg aattttcagt tagaggatga ttttcgggaa gcagtccatc ttcatccaat
     1141 tctactaatc gttcatcctg ataattttcc ggacgataaa actctacacg attatggtag
     1201 atatcataaa gaccttctag ccctttaccc ataccgattg gccaattcat cggataagat
     1261 tcgatatcta atagctcttc taattcttct aacaattcca atggttcacg accatcacga
     1321 tctagtttgt tgataaaagt aaatatcggg atgccacgtt tttttacaac ttggaataat
     1381 ttctttgtct gtgcttcgat cccttttgca ctatcgatta ccatgaccgc actatccacg
     1441 gccatcaaag tacgataagt atcttcagaa aaatcttcat gtcctggtgt atccaagata
     1501 tttactcgtt tcccttgata atcaaactgc ataacagaac tagtaacaga aatcccacgt
     1561 tgtttctcga tttccatcca gtctgatttg gcaaaattac ctgttttttt gccttttacg
     1621 gtcccggctt gacggatagc cccaccaaac aatagtaatt gttctgtaat cgttgttttc
     1681 ccagcatccg gatgggaaat gatcgcaaaa gttctgcgat tgtccacttg ttgctttaat
     1741 tctggattat tcataaccta actccatttc ttccattcaa ttatttatat tttctctagg
     1801 cacagattgt ccgatctctt aaagaaagac aatttcatat agggctgtga caaaagccgt
     1861 gtagcagtct ctttttccga gtaaacggcg ttctagagac agcctatcag caataagccc
     1921 acccaagcca aaaatacgaa aagctgtttt tgcttggctg ctcttattac ttaatgcagg
     1981 atagcttttt cacaaccttt gtcaactact acaactgaat cagtatagca tggattcatc
     2041 gaatgaaaaa gccaccaaac cataagagtc aaatattttt ttgtcagctg actaaaagaa
     2101 aagacgtgtt gcttgtttat tcaagcaaca cgtctttcat aaccaagttt ccaattatct
     2161 tcttggctcg tcatcttcga attcttttct gaagtatctt cgttcttctt caggttccgc
     2221 ttctgtttct tccggatcat gaaaaatgac acggatttta aggattcgag aaccttccat
     2281 ttcttcagaa accagcgtaa tattatcgac atcaaacgat aacttttcgc cttcatccgg
     2341 aatcatgcct aatgctgtaa tcaaataacc agccatcgta tctacatcac tcatatgcag
     2401 atcactgtca aaagcttcat tgaactcatc aatcagcata cgtccttgga tgatatattc
     2461 gtgttcatcg attttttcat aaagattctc aacttcatct gattcatcat cgatttctcc
     2521 gacgatttct tctaaaaggt cttctagtgt tgctaaccca acgacgccgc catattcatc
     2581 taataaaata gccatctgat tctgcgtctt tttcatttca tataacagat catcaataaa
     2641 gaccgtttca ggaacgaata aaggctcctg catgattttc ttgatatcta aattatcaaa
     2701 accgaattta tgggcagctt ttagtaaatt ctttgtatga aggattccga ctactttatc
     2761 tttgtcttcg ttataaacag ggattcttga atagttttcg gataaaactt cattcacatt
     2821 ttcttccaca gtgtcgttga tatcaatcat aaatgcatcc gttcgtggga ccatgacttc
     2881 tcgggcaact ttcgtatcaa gagaaaaaac cccttggagc atttctaatt cttcattatt
     2941 caatacgcct tcgttttcaa gcatataacg catttcatct ctggtcattt ttgaatcttc
     3001 atcatcaaaa gtcatcggcg tgatacgcgc caataaactt gtggaagcag aaagcagcca
     3061 gacgaagggc ttagctacta caccaatcaa gcgtatcagc cctgaagtga actttgcgac
     3121 ttcttctgat ttatttaaag caatacgttt agggtacaat tctccaaaaa caatcgatac
     3181 ataggtcaaa atagccagca caatgatatt tgcaatattt tttgccgctg tgccgccacc
     3241 taataccgga gctaaccgtt ccgataaagt attagctagt gaagcaccag acaaaatgtt
     3301 gaccaaagtg attcctactt ggattgtcga taggaaatta tttggattct gcaatatttt
     3361 taaaagcttt tgagatttca catctccttc atcagctttt tgctcgattc gattcttatt
     3421 gatcgaaaca acagcaattt cggaagctgc taaaaaagca ttgatcaatg tcaaaacgat
     3481 caataataaa atctgcgcta acagcgactg actctcaggg tcagcattca taatcgcatt
     3541 ctctccttat aaaattaatt tttagtttac gatagaaaaa aacgtggcac tatagccacg
     3601 ttaaaatggt atcacaaagc aattttcttt gcaataaaaa caagtgtact aaaactacct
     3661 acgaggtcgt agcaattttc ttcttgtttt cattttgctt gtcttctttg acgattttga
     3721 ttatatagga aatgatcgtc ttgcagattg cgtaaaaagg aacacctaag aagataccaa
     3781 gcaatccggc aatatttcca gctactagaa gaacgataat gatcgttaat ggatggattt
     3841 tcagtgattt cccaattaca ttaggataaa tgatattccc atccaactgt tggacgatta
     3901 gcacaaccac acaacaaagt aatgctttta ccggatcatt gaagactgtt actagaaatg
     3961 caggtgctag tcctagataa ggtcctaaat atggaattag gtttgtcaat cccgcaatga
     4021 ccccaaataa gaaagcataa tccacaccaa ttaccatata tcctaaaaat gtgaaggtac
     4081 caacgaataa acattcgatt gcttgtccgc taatgtaatt agccaatgtt ttattcagtt
     4141 gccccaataa ctccacaatt tggtttcttc ttttttctgg gaaaaactgt tggatgtttg
     4201 gcactagttt ttctccgtct ttcaacatat agaacaagat gaatggtgct gtaatgatta
     4261 ctacggttgc cgaagcgatc gttccaacga tcgaacctaa actattggat actccactaa
     4321 ggaattgctg aatgattgtc ccgtaagaaa tgtctaattg ctcaaaatat ttattgatat
     4381 ctacttcctt gaacaaaggc aattcggcta cctcttttaa ccagctttct acctgtttga
     4441 tgaaatcagg catattagat gccaatgagg aaatttgctg tactagactt gggatgacac
     4501 taagtaaaat ccaaataatt gctgcaacta aaaggagcag cacaattgcg acagcccata
     4561 ttcttttgat ttttgctttc atcaacagct tgacaatagg attcaataaa tagtaaagaa
     4621 agcctgcaat cagtacaggt gcaaataacg tggaaaaaaa tgtcccgatc ggttcgaaaa
     4681 taaagtttat ttctgatgaa atcatgatca atgcagctat gatcaataat tcaagcgacc
     4741 aaaacattag tttagactgt ctaagttttt caaacaatag atttcctcct ttctttctta
     4801 ctagtatata ttatattcgt taaaaattct tttattcttt ttttattttt gaaaacttgc
     4861 catacttttt atttgtttta ttttccagta tactatacga aaggaaggtg gctagaccat
     4921 gaaagttgta caaacaaaag atacaatgag caatatttac ttagatgctg tacggatcag
     4981 gcatcaagtt tttgtagtcg aacagggtgt gcctttgtct cgagaaatag acaaagatga
     5041 agcccattgt atccacttcg tcctgtactc agataaaaaa gaaccgcaag gaactgtacg
     5101 tcttttgcct ttagaaaacg gaaaaatgaa attacagcgg atggcgattc tttctgaata
     5161 cagacaccaa gggttaggaa aaatactgat tgaagaagca gaaaactttg ctaagaatca
     5221 aggctataat acgattttat taggggcaca gtccacagct gaaacttttt acgaaaaact
     5281 aggatataca gcttacggcg atccttttga agatgctggc atgcctcata tttatatgaa
     5341 aaagacttta taaaaattta tttcgaaaaa agcaaaaaac agcaagcttt tgagaaaaat
     5401 ctctcactgg cttactgttc ttttttgtgt ttacttacgc gacaattgct tgtaacggtt
     5461 ataccaaata tcaatatagc cttccgagaa cgggccatct ccattattga tccagtcaac
     5521 cagaatcttc acattttctt ttaggatgaa gtcaatatcg ttcggataat tcatcatttt
     5581 gctgtgcatc tcatattcat cgacatccaa tagtcgtttc tcgccgtcag gaaatacctt
     5641 gatatccaag tcatagtcga tatatttcaa ggcctcatcg tccagcaaaa aaggtgaagc
     5701 tagattacaa taataggaaa cccccttttc tctgatcatt gctattacat tgaaccagta
     5761 ttttttatga aaataaacaa tcgctggttc gcgcgttacc catcggcgtc catcagattc
     5821 agtgactaat gtgtgatcat tcacgccgat catagagtgt tcgcttgttt ttaataccat
     5881 ggtatctcgc cacgttcgat gcaatttgcc gtcgtgcttg taactttgga tcgtcacaaa
     5941 ctctccttct tttggaactc ccatagcaac ccttccttaa gcagcatttt gtgctttgtg
     6001 tttgggtgtt cattacaccg acatcataat tattatatca ataaaaatga gctttgccta
     6061 gaaaggattc ctattgtttc gatttttctc taactgatct accagtttca tttggacctt
     6121 aggaaaaacg accgaatcaa atgcatcagg cttcaaccac ttcgaagttt tgttctcggt
     6181 aaattcttgt tctgctccct cggttgcccg tccgtaaaaa aggagaacat gccatttcag
     6241 atgactaaaa atatgagtaa cttctcctaa atgtcgtgtc tgccaaacaa agagttcctg
     6301 cttttcaaat gggagatttt gctcttcttc tgctactagg tcatcaaaca gatcgatttc
     6361 tggctttgtt tcccattctt ttttcaatcg ctcatactcc tcttgtgtca cttccatcat
     6421 tggaaatgtc cacatattcg ccagcaattt ttggctatct ctttcttcaa aataatacgc
     6481 tccagaatga ttttgaagag ccgcactgat ataataaacg tcttttggct ttgctttttt
     6541 tgtttttaca ggaaaactcg tttggattcc tcgtttgtta gctaaacaaa aggcttggat
     6601 cggacaagtc tcacactttg gagaagttgg tgtacagata gctgaaccta gatccatcat
     6661 tgcttggttg aattcacctg gatgcttttc atcaatgatt ttccgcatcg cttcatcaaa
     6721 gattttcctg ctagaagctt ttgcgatgtc tgcttcaatg caaaacaatc tgctgactac
     6781 tcgcatcacg ttgccgtcaa ccgcgggttc tggaagtcca aatgcgatac ttgcaatcgc
     6841 gcctgtcgta tatggtccta ttcccttcaa tgagctaatt tcttcaggcg tttgcggcat
     6901 ttccccatca aattctgaca tgatctgttt agcagcagct tggatgttcc gggctcttga
     6961 gtaataacca aggccctccc atgcttttaa aagcttttct tctggagcat ttgctagctc
     7021 ttcaattgta ggaaaccatt ccatgaaacg ataaaaataa tcgatcactg tgtctactct
     7081 ggtttgctga agcatgatct cagagatcca gatacgataa ggatcacgat tatagcgcca
     7141 aggcaggttt cttttttctt gttcatacca ttgaatgaac tgatcttgaa attcttttgt
     7201 ttctttatct gtccattttt cccattcttt attcactttc ctcttccttt tccgtaatga
     7261 atcgctggat catatccaaa tcaaatcctt tttgatataa gctttgcttt acttttagat
     7321 ttcttttttt aggttcaaac cttaaatttc ttcgccatag tttatctcct tgtttgacta
     7381 aataatcata ttctatttct tgatctgctt cttgcggaag atcagccatc gcttgttgga
     7441 tcacttcttg agaaaaacct ttcgtcatca atgtttgttg gatttttctc aataattctt
     7501 tttggctttt tccgcgattt tttccaaacg ctttttcggc tgtctgatgt gcattttgaa
     7561 cttggtcttc aaatgtatat tggctgagcg cttgttcaat gatttcaggt ttcaaacctt
     7621 tttgctgcat ctgctgcgct aatttctttg gtcctttgtc acttaagcgc atattcgtgc
     7681 gcacataact ttccccatag accaaatcat cgacaaggtc caattctttt aagcgggcta
     7741 cgattttatt tcgatcttct tgaaggattt ccttttcttt taagtatgtt cggatttctt
     7801 tttccgatcg caactgataa cttaagtaat tcattgctag gtgaaaaccg aaatcagctc
     7861 cgctactctt cttgatctct tggatagttt cttcgtctaa ttcccgtcct tttaataatc
     7921 gatagcgtac taatacatct tcagatactt gtaaagcttc tccttcagat aaatctacgc
     7981 gatagaaagg gcctttgcct ttactgattc tcgtaacggt taacataaac atcctcctcg
     8041 aacatatatt ctctatcata ccacagaaaa tccccgtaat caggtattat atgttagaat
     8101 aaaccacaga aaaaagaaaa agggagatac ttatggcaga aacaattgtc aaaactggac
     8161 aaacggttcc tttgaaaatc aagcgtttag gcataaatgg tgaaggaatc ggttatttca
     8221 aacggttgat catatttgtt ccatatgcac taccaaaaga agaagtacta gtgaaaatca
     8281 cgaaagcaac tcctcgctat gcagaaggag aattaataaa agtcaaaaaa acaagcaaag
     8341 atcgcgtagt ggcaccatgt cctgtttact atgaatgcgg tggctgtcaa ttacaacact
     8401 tagcttatca agctcaattg gattttaaaa aggatctgct tttgcaagca ttggaaaaat
     8461 tcaaaccagc aggctttcga tcttatcaac ttttgccgac gatcgggatg gatgagcctt
     8521 ggcattatag aaacaaagca caatttcagt tacgaaaaaa taagcagact caaaaaattg
     8581 aagctggttt atacgaagca aactctcatc agttagtccc tataacagac tgcttggttc
     8641 aagaacctcg aacacaaaaa gtaatgaata ctgttgttca actattgaat aaatttgatg
     8701 cacccatcta tgatgaacgg acaaacagtg gaatcttccg cacaatcatg gttagaatcg
     8761 gtgttaaaac tggggagtta caagttgttt ttattactcg cagtaaaaaa ttcccgcaaa
     8821 aaaacgcaat gatccgtgag atcaatgaac gactgcccga agttgtttca atcatgcaaa
     8881 atgtccaggc acaaaaaacg tctctcgtga tgggggatga aacgatccac ttatggggaa
     8941 aagaagccat tgaagaacaa atcaatgaag tgactttcga tctttctcct cgtgccttct
     9001 ttcaactcaa tcctcagcag accgaattgc tttatggcga aggaatcaaa gcattagatg
     9061 tccagcctaa tgaaacgatc gtggatgctt attgcggagt tgggacgatc ggcttaagtg
     9121 tagcaaaaca agccaaagaa gtacggggaa tggataccgt tcctcaagca atcgctgatg
     9181 caaaaaaaaa tgcggaaaga ttaggatatc acaacactcg atatgaagtc gggacagcag
     9241 agcaagttct gcctaaatgg ttgaatgaag gttttcagcc tgatggtatc atcgtagatc
     9301 caccacgtac gggtttggat aacgcattga tccaatcatt attgaaatac cctccaaaaa
     9361 aacttgttta tatctcctgt aatgtttcta cacttgccag agatctggtt aatttaagta
     9421 aaaagttcaa gattgaatat ttacagtctg tcgacatgtt tcctcagact gcacgttgtg
     9481 aagttgtcgt gaagatgacg agactcgctg actaaatgaa agaggttgtg aaaaagtctg
     9541 ccctgtatca agtaataaga acagccaaac aaaaatagct cttcatgttt tatgaatacc
     9601 gtacttgtga ccgaagagtt aacaggagga cactatttat ttaaaaaaag ggttgtgaca
     9661 tagtttttgt cacagccctt tttgattatt attggatatt cacgtctatc acttgataaa
     9721 atgcatttgt cgtatcgctg atattccata ctgctaaaat cacattatat ccttttcgat
     9781 cctgcggaag tgtgatcgtt tggttgactg tttttggtgg aacagttgct ttgtcgtcta
     9841 tttttgtgat caattcaaaa tcactaaatt tcaaaggttg attaggattc caagttggtt
     9901 tggtaatgta gtaatcccaa gtacttgtcc gatgttgtgc tgttaaattc caagtaatat
     9961 ttaatgttcc tggattaacc atgtttttat gccatctgtt agatgtctgt tcatctaaag
    10021 gctcaaagcc gctgactcct gcactagcaa tttttccatc aatgaatgta tttttggtag
    10081 cttcgatact ttgcggttcc cattgtgctc ttccgacatt ttgatttaag tttcctgctg
    10141 ctgatgttcc taggtatgca cggcttcctg gagaagtaat ataaccatgc gcaaaacctg
    10201 gaatgctaaa accgaacaac cctaaactaa ctaacccaat aaacaataac gtactaatct
    10261 ttttcatgta gaaacctcct tcatttgata accacattgt aaacgcttta ttgtattaac
    10321 acaaacatta tgcttttata attttatgtt gaagtaaaaa aggtgaaaaa agtaagatgc
    10381 agattataac agaagtagac gagaaaagta caaatagaaa acatgagaac caaatcatat
    10441 gatctttttc aaaaacatca ttcatataaa caaatgttca actttttaat aacaacaatc
    10501 atcaattttt ttcttacttc acaaactaaa tacaggtctt gctgtgagcc ttcatggtaa
    10561 actactatta tagaaaggag ttagtaaaat gaagatgata aaagatattc gtttttgggt
    10621 ttgtgttata ggaattgtca tattaggctt tttatcaggg ttgcttagcg gaaatcccgg
    10681 agaatattac tattctttgc aacttccacc atttgcacca cctagctgga tatttgggcc
    10741 gatgtggacc ttactgtata tattgatggg tatttctctt tatttgttac ttaatcacaa
    10801 caacaaaaaa cagcgaaaca atcttgtcgg tctttttgtg attcagttca tttttaattt
    10861 tatatggtct gctctatttt ttaatttaag aaatattttc attgcagcaa tcgatattac
    10921 acttttagtt attttcttgt cagtgcttat gtatcagttg tggctacatc atcgcctggc
    10981 aatgtggctc atgattcctt attatttatg ggtactattc gcaactctat tgaattattc
    11041 aatttatttt ttaaactaag cgctaaaaag agacgtcctg taatttaaca catagaaaaa
    11101 gtgccaccta agttagattt tttgtctaat ttaggtggca ctctttatta accaaactat
    11161 ttttttctag tacccacttg ttgttccata ctcattattc tggtcataac cataaccatt
    11221 ttgttcctgt ccagcgtaag gatcataact ttgagtatct tgacttggtg tatagtactg
    11281 acttgtatct tctgtttgct gataataggt attcggatct tggtactgat catatgtgtt
    11341 ttctgaatag gaagaataat cggtgtaccc ttcactattc gatccatcac cgtttgtcga
    11401 atcgccatct tccgaatcat caaagaacat cattgaagca ttctcatttt tcaaattagg
    11461 taatgtatcg cttgttttta tatttaattg tttcttcagt tcattttgga tcgaaagaag
    11521 atcatttttt cctaaaattt gatagtagat atcattgatc atttgtcctt cgccttgcaa
    11581 ctggtcttgt ttgattgttt caaacgcagg ggtgtagttc gtcgctacat caagcatgtc
    11641 atcccaatct aagtccgtcg tcacattgtt ctcaactgct tttaaaattt ttctataatt
    11701 gcttacgcca tctaaactta aaactttatt gacgattttc gtcactactt cccgctgacg
    11761 acgttgtcga ccgatatcgc cttctggatc ttcgtgtcgc attcgagaat aggctaaacc
    11821 tttttctcca ttcaatgttt gttcacctac tggtacatgg acaccatcca aagtaaagtc
    11881 aattttatta ttcactttga tgccaccgac cgcattgatc aaatcttcca tgccatccaa
    11941 attgatcgtt acataatgat caataggaat atcgagcagt tgctcaattg aatccatggc
    12001 catttcgaca ccaccaaatg cataggcatg atttaattta tctaccgttc cataacctac
    12061 gatattcgta taaatatctc ggtccaaact gatgatcgtt gatttttttt gtttcggatt
    12121 aactgttact accatcatcg tatcagaacg tccttgttcc gttcttccta atccaccggt
    12181 atctaaacct aatagcaaaa ccgaaaaagg ttctttgtca tcaatgctta ctctctcaga
    12241 acgtttggag gatattcgat tagagttttt agaaattttg tttactgttt ggctcgcttc
    12301 tcccataaat ctgattccgt atgctgataa tccggcaatc agcaaaacaa atacccctaa
    12361 caaggttaat agaacttttt gccatcgctt cataatacac ctctgtttca cattttctaa
    12421 aatattgtct catataactg ttcaatcagc aaattcactt tttaatcata agaaaacttt
    12481 aaaaaacatt tttgtgtaac gattactatt ttttaaaatt tctcaaaatt cttaactatc
    12541 ataccatacc gtgaaaaaga tcgaaagacc gaatacacgc agttcgcaat cgttttatct
    12601 ttttttacgt attttttcca gaaaaagcga acaacaaggt attttccaaa taatcgtgca
    12661 gtaaaatgat cgcacatacc tttcccatct ttttaacagt ctttgcctac cgttcagatt
    12721 atcattcgat ttcacgaaaa tttcttccta tgactggtcc ttttgttaaa cccatcttct
    12781 attcgtttta tttccagctc actgcatggc tcttaaaatg actcgaacta gtgatttcat
    12841 taccatcatc ttttaatgga tagatatgct cctcttcaaa gtaaaacgat tttcttttaa
    12901 agtaatagaa ttgacatggt aatgagtatg tatactagtg gtttcacaaa tggtctttta
    12961 ttcaacatcc aaaaactgaa atttctttaa aatccattac taacgaagaa aatgttcctt
    13021 gcttgataat tagttaggac atgtcaagtg aacaaatgca tttacttagc caaaaaatct
    13081 aaaaacttca taaatagaca aaaagacacg actctctaag aagtcgtgtc tttattcaac
    13141 aaattattat aatttagtca cttcagcagc ttgaggtccg cgtgcacctt caacaatcgt
    13201 gaattctact tcttgacctt cttctaggct cttgaagccg tctccttgaa ttgctgagaa
    13261 atgaacaaat acgtcatctc cgccgtctac tgtaataaac ccaaatcctt tttcattgtt
    13321 gaaccattta accgttccgt gttccataaa tgtatagcct ccaaaaaagt tcttgcaagt
    13381 aaccttgcaa gaacattata cccaatttag aaaacgctag caagctttcg ctcttttatt
    13441 ttccaaatta tttttaagac tgatatgacg gaggaaatgg ttattttgct gcttcgaaac
    13501 gtttgtttac ttcatcccaa ttaataacat tccagaatgc tgcaatataa tctggtcgga
    13561 catttttata tttcaaataa taagcatgtt cccaaacgtc caaacctaga attggtgtct
    13621 ttccttccat caatggagaa tcttgatttg cagtagaggt aatagctaat tttccatttt
    13681 ccattacaag ccaagcccat ccagaaccaa atcgtccagc ggctgctttt ttgaattctt
    13741 ctttaaaaga agaaaaatca ccaaaagctt cattaatcgc ttcttttatt tctcctgtag
    13801 gttcgccacc agcatttggt gccatgattt cccagaaaaa tgaatggtta gcatgtccgc
    13861 caccattgtt acgtacagct gtcttgatat ctgttggaat agcgtccata tcagataata
    13921 attcttctat tgttttttcg cctaattctg ggtatttctc aattgctgca tttaaattcg
    13981 tcacataagt attgtgatgc ttatcatgat gcagatgcat cgtttcttcg tcaatataag
    14041 gttccaatgc gtcatatgca taaggtagat ctggtaatgt gtaagtcata aaaaaattcc
    14101 tcctcaatgt tttctttata atgtctacat tacaaagagt aacaaagaga aggaaattct
    14161 tcaattgtta tgcctagtcg tctttttcac tttgacctac ttcttcttca aaaacctcag
    14221 tttctttaaa aataaacaac ttgctgaaaa tatagttcgc taggataaca ataatttgag
    14281 tgatgatttt cgccacatac tcattgctgt tcataacatt gatcaacaat accattgccc
    14341 ccatgtccaa tccgtaagaa aggatacggt agaagaagaa cttaccgcat tcaataagtg
    14401 catgtgtaaa attcggtgat tttgaatgga atacccaaag tttgttcgtt acaaaagcaa
    14461 acaatacaga acaaaaccac gaaatagaat tggataaggc catacttaaa tcaaaatatt
    14521 ggcgtgcgag agcaaaacta acaaaattta caatcgtagc caaaccacca aaaaagagat
    14581 agactagtat ctcccaatat ccttttgctt ctaaatatcc ttttaactgt cgatactgct
    14641 tcatgagcca acctacctta ttaaaaattc ttatttatca tactctatat ttcaataact
    14701 ttcatcttta tttaaaaaaa ttaatttttt ttgatccttt gcctttgtct ctgcttggat
    14761 cgtcacatgt gtaataccat attttaattt taataaagaa attatacgag gatatatctt
    14821 ttcaatctcg cttagtagct gatcttcaaa atcaatatgt ccgtcgaaaa taattatctt
    14881 ttcgctatac tgccaaatat ggacatgatg aacattcttt accccatcga ttgcttcaat
    14941 ttcttgttcc attttttcat agtcaatagc aggtgcactt tgtatcaaaa tagcggcagc
    15001 ttttttgatc accattatcg cttctatcaa tatatatatc gaaatgatca gtgtaataac
    15061 tggatcgatc cagtagatat cgaaaaaacg gatcaaaatt gctccgataa tcacacttac
    15121 cgaagacagt gtatcactta acaaatgcaa ataactagat tttatattca aactttcatc
    15181 tgcttcttgt cgtaataata atactgagaa caaattcgcg agcaatccaa taactgctac
    15241 tgttagcatc aaatgactat ttatcttttc tggtgaagtg aaccgcttga atgcttcaac
    15301 ggttaaaaac aaggataata tgatcaatat gaatgagttg atgaaagcac tcaatatttc
    15361 taaacgctgg tagccatacg tacgtttttt atttgcttct tttcgggcca ttttttgtgc
    15421 cccgtaagaa aaaataattg ccaatgtatc actaaggtta tggaaagcat ctgaaacaag
    15481 agacaaactc cctgacataa tccctccaat aaattctgat aaagtgatca ccgcattcaa
    15541 aaccactact ccaaatagtt tttttgatga tctctgtggc tgtgtaggca ctattccttc
    15601 ctccatttta tttatattgg atagtcttta gacaagaata acattttttt gatatttcac
    15661 caatcattca gatgtttccc taaagcactt gtatttcact tgatacttcg ctagaattgc
    15721 aagtgatgac actactagat gaggagtact tatgacaaca aaacaattaa ttatcagttc
    15781 ctttatacga ttcaaagacc taaaggagac gagaaatgtc ccattttgga aagccattgt
    15841 ctatctttta gttttgagta taattatggc tttgccaatc tcttttcaag tttttcaagt
    15901 cttaaacaac attcaattgg atgggcaaaa gattgctact cgcattcctg attttacaat
    15961 ccaaaacggg cagcttcaaa ccgaagaaaa agaaggattc atttaccaaa caaactccat
    16021 tatcttcaca tttgatccag agggaaaacg aacagaacag gacatctcta ctgatttgat
    16081 ggggaatttt gttagtgtcg ggatgttaaa aaacaaactt atcatagctc ttcctaatac
    16141 aggcacgact tctgctttgt tgaacaataa ccaattagaa cttccctaca ccaatgaatc
    16201 tttaaaaaac ttaacaggta aggagctccg ttctttttta agtaaagcat ctataccact
    16261 ttggataaaa gtcctgacat ttatattttc tatttatcct agttttctga atctgctgat
    16321 cactttgcta tttaccaaca ttgctgcttt tctctatgca cgctttcgac taacaaaagc
    16381 tacttttctt gattgtttaa aaacattgat ctattcaatt tcattgccta ctataattgc
    16441 aacaatcttg atggtctttc ttccttcttt tgacacaagc gccttcattg cttttgcagg
    16501 aatctttatt tttgctcaag ctgtcaaagg atgggcaaaa atatcgatca attaagaaat
    16561 aaagacaact cgttatttct acttcaatag acccttttag aggttgtgac atttttttgt
    16621 cacagcccct tttttatttg aaaaatattt ctttctcaaa aaacaagcaa ggcgcttcaa
    16681 agttcttctt gtatgcgcta cacttagagt aaagaaaggc gggaaattaa ctatgtcaga
    16741 aagaatcatt tatgcagatc gtccatttcg tgatgaattt atcgaagaaa tccatactat
    16801 agcgcctggt tatatcttta aaacagaatt acaaccagag gatctacaaa atgtagagat
    16861 cagtttgggc tggaactcag actaccaaga aaaactatta gcctcttcta ccttgaaatg
    16921 ggtccaatct atctcagctg gtgttgatca gctacccctt gagacttttt caaagaaaca
    16981 tattttactt tcaaatggta gtggcatcca cagtgaatcg attacggagc atgtcttagg
    17041 aatcatcctt gggtattctc gtggtctttt tcaagcacaa aaagcacaga gtgacatgaa
    17101 atggttaggt tcatcggtcc attaccaatc tatccaagga aaaaaaatac taatcattgg
    17161 aactggccat attggaaaaa tgattgcaaa aaaatgcaaa gcattagaca tgaactgcta
    17221 tggaatcaat acatccggtc atccagcaga aggaatggaa aagacttttt ctttaaatca
    17281 actaaaggaa gttcttcctg aagtagattt cgtagtcaat atcttacctc taacggagga
    17341 aacaactggg ctctttgatc agcatatgtt tgaacacttt gatccaaaat ctgtattcat
    17401 caatgttggc cgtggtgctt ctgttaagac acaagatctg gtgcaggctt taaataacca
    17461 gcaactttcc tttgcagcat tagacgtttt tgaggaagag cctcttccac aagatcatcc
    17521 tctttggaaa atggacaatg tactgattac ttcccacatt gcaggtttga cacctgattt
    17581 tcaaaagaaa ttaatggcta tttttttaac caatctgaaa agctactttg ccacccatga
    17641 gcttcaaacc aatcaagtaa aattgtctgc aggatattaa tagctgaaaa caatattttc
    17701 aaaagaacaa agagaaggtg acaaaacttg tgtcaccctc tcttttttta ttgtttttta
    17761 aagtgatagg ttggctcctc caggttggga tcataatcaa caagtaaatc atatccttta
    17821 aagaaccatt catctgcttc ctcgataaaa aaaagtgtcc catccatttc cactttcttt
    17881 acaggatgtt ctggctgttc aactgacatt ccaatcgaaa aaccatcatg aacctctgtt
    17941 ttgccgtaga cttttccaaa aaaacgtatt ccgtaagttt ctggcagatc aatttctgag
    18001 tcaaaccact tttgtgcttt tggcgttacc gttaattcca tcccaacaac ctactttctc
    18061 ttttctttct attgattcat cttatcgtct agaatatcca catcgcctgt ctctccaatc
    18121 accagtagat tatcattttc gcgaacatat tcatctgctg caggagaagc gataaccgtt
    18181 tgatctactc ttcgtatggc gactactgtt aagtcaaaac gttgcctgaa atttaattca
    18241 gctaatgttt tgttgaaaaa tttaggattg gagactcgga tttctgctaa agaatattta
    18301 tctgacagct ctaagtagtc tagaatattc tttgatacta agttatgtgc aatccgtatc
    18361 cccatgtctc gctcagggtg tacgacacga tctgctccga tcttctctag tacacgggca
    18421 tggtactcat tttgggcttt tgctaaaata ttcggtacgc ccatttcctt taccatcaga
    18481 gtaactaaga tactggcttg gatatcttct ccaatagcga caattacatg atcgaaattt
    18541 cttataccta atgaacgaag tgtcatctca tcctgtgcat ttgccacaac agcatgagtg
    18601 gctatattca tatattcatt gactcgatct tcattactgt caatagccag tacttcttgt
    18661 ccagcttcga tcagtgtacg gcagatgctt ccgccaaatc gtccaaggcc tacaattgca
    18721 aaattttgtt tcaccatttt cctcctttag tatcttcttt tctcagtata acaaagcctt
    18781 tccaaaaaca aaagcaagca tcattattgt aaaaaagcca agaaaaataa tagttatttt
    18841 ctcagctttt gattatcctc tttatcatcg tttttatttc accaaaccat gtttaaacgc
    18901 atagatagct gcttgagttc ggtcttctac ctctaactta gctaatatat tcgacacatg
    18961 cgttttaact gttttcagtg tgataaacaa ttcatccgcg atttcctgat tacttttccc
    19021 ttcagaaatc agcatcaaaa tttcattttc tcgattcgtt aattcttcat gcaatactgg
    19081 atcatttcgc cgactcattt tttccatcat ttttgttgtc acttcaggtt ccaatactcg
    19141 ctcgccgcgt tgcgtagccc ttatagcatc agctatttca tgtgcggtag acgtcttcaa
    19201 caaatatcct gctgctcctg cttcaattgc aggataaact ttttcatcat caataaaact
    19261 tgtgacgata atgattttgg cttttggaca atcttttaag attgcttttg ttgattcgat
    19321 gccatccatt tcttccatga ctagatccat taaaatgaca tctggtcgaa gtgccatcgc
    19381 cttttcatag ccttgacgtc cattttcagc ttctccaatc acttcaatgt cttcttggat
    19441 agataaataa gaagagacgc ctaagcgtac catttcatgg tcatctacta ataaaacttt
    19501 tatcatcttt atctatgcct ccttcatcaa agggaccttg atctccacgc tcgtaccttg
    19561 ccctttaaaa ctaatgattt ttactgttcc accaatacca gctactcgtt ctttgatatt
    19621 gttcaatcca taacttcccg ttttggtttc attcatatta aatcctgtcc cgtcgtcaat
    19681 gatacgtaat aaaagattgt tatctatttt atgcaaatag acttctaatt cattagcctt
    19741 agcatgtctt aatgtgtttg ataacaattc ttgaacgatt cggaacaagt gatcctcaat
    19801 ggaactagtt aattttacat cttctacatc ccattttagc gaaatctgga ttttattctg
    19861 caattccttc aataactgct caatgccttg ttttaaactt ttttcttcta aattgactgg
    19921 acgcagatga agaagcagcg cacgcatctc agactgggaa gcgttgatga tctctgccac
    19981 cattttcaac tgcttacgga acatttctgg tgtctcactt ttttctgctt gctccgtcaa
    20041 tgccgacatc atcatcatcg ctgcaaataa ttgctgtgaa actgaatcat gaagctcacg
    20101 agctaatcga tgcctttcta attctaaaat ctcttctttg gtttgtccat ctacatattg
    20161 aggacggcta gtcataatct gtaattcact ggatacttcg atcatttttt cttttatttt
    20221 agcaatttcc atatcaatat ctttgatgta cagatcatcc gacgcgcttg ggatcggctg
    20281 atgcaaaagt ttggattcat aattgccaga agaaagtgca cgaagctttt cttcgatttt
    20341 cccgtatttt gccttttgaa taagtgataa caatagaaat acgatcaatc ctacacctaa
    20401 tgaaattgcc atcaaatgga agataagcgg aacgtataat aaacgcgcac gcagcaattc
    20461 tccccaccaa tgactttggt tactggcata aaaataagtg aaaagtgaaa atagaataat
    20521 aaggaaagcc gcaatccccg aataaacagc tagcattgct ttggatattc ttcccatcat
    20581 attcgtatca cctcgacatc tccaaccaag gtattggtaa tgatcttcaa tctgcgagga
    20641 ttttcatcat aatcactgct gcagattttg atctgttcgt tttttaagga aaactgctct
    20701 tcttcaaaca agacatttcc tactaatgtc gcatgctcta aacgaatagc cactcccagg
    20761 ggtactaaga tccgtgttct gcctatccct tttcttacaa tcacaatatt atcatctttt
    20821 ggcaaaagtg tattacccaa atcgatgata gtatcaccag aaatgatggc aatgttgata
    20881 tcatcccatt catagacgtc gtttccaata cgttggttac caaataattg ctgtctttta
    20941 cgctcattat tatgtgtttt taattgctct gtttggacca tcatgatctg tttttttcgc
    21001 caaaaagcat tttttgtcaa atctattcct gaaatctcta cacctttcag cccgataaat
    21061 aaaacggcca atacaaccat catccataaa gcaggactgt tgactaagct aaaaaaaatg
    21121 accaagctcc ctaagatcaa ttgaaaattt tgaaatttag tacgaggata tttgcgcata
    21181 gctagatata tattgaaaat accgaatatt actaaaagca aaagtccagt attgtttacg
    21241 atttgccaaa cagcaaataa cagcagcagt gcttcgacta ccacaaaaaa acgccaagaa
    21301 ctattcatcg ctttttctcc ctcctgacac attctcattt catcatacct atttttttga
    21361 ctgttggcta taggacttta gatgtaagat cgttcagtga aacctgcaat actcaaagag
    21421 aaaagatcta gagtgcgtca tcagtaagat gaactctcta gatctttcag tcagactacg
    21481 cttgttctac tttgacgatt ttaactaaca tatcgccacc tggtgtagca atggttactt
    21541 catcattttt acgtttgcca atcaatgctt gcgcgatagg agaatcattg gaaatctttc
    21601 cagaaaacgg gtctgcttca gcacttccga caatcgtgta ctcttcttca tctccatcag
    21661 gcaactcgat gaacgtaact gttttaccga tcgatacctc atctgcatct acgccgtcat
    21721 tatcgatgat ttgtgcaaaa cggatcatat tttcaattgt tgtgattctt ccttcaacaa
    21781 atgcttgttc atctttagct gattcgtatt cggagttttc ggaaagatct ccgaaaccgc
    21841 gagcgatttt gattcgttca atgatttctc cgcgtttaac tgtttttagt tcttctaatt
    21901 ctttttctaa tttttcttta ccttctagag tcattggata aactttctct accatgtgta
    21961 acgtctcctt ttttcgataa aataccgcaa aaggtaatct tttgaggaat atagtccaaa
    22021 aaagattacc acgcttttct taaaatgtaa atgtttttct aaaaatgatc aatgactagc
    22081 ccctgttttt ctaagaatta tttacatatt tttgcactag ttccatatgt tcttcatacg
    22141 ttttagcgaa ataaacattt ccagtgctcg tatctgctac gaaataataa tagtcatttt
    22201 ctgctggttc taatactgcc ttgattgcct tttcgcttgg attgtcaaaa ggaccaggcc
    22261 catagccggt attggtatac aaattgtatg gtgaatctac agctgtatct tcatacgtca
    22321 ccaattcttt atgttctcct aatgcatata aaacagagat atctgactga agtggcatat
    22381 ttgccttgat tcggttaaag aagacttgtg caatattttt acgatcattt tcttttacgc
    22441 cttctttttc aactagagaa gccaaagtta aaacttcttg tacagtcaaa tttttctgtt
    22501 tgatagctga ataatagttt tgcatgaccg tatttgtctt gttcaccatc tcaatcacaa
    22561 gatccttaag cgaagtactc gtgtagtaat cataagtagc tgggaataag tagccttcca
    22621 aacggtattt tgtgttctca gctttagagg cactttccaa caattctggg aatttttgat
    22681 acaactcgtt aaagaaagtc tcatcattta ctaaatctaa aaattctttc ttgtctttac
    22741 ctgttacctt cgctacccgc tcagcaatct gatcgatgtc gtagccttct ggaatggcaa
    22801 ttttcccatc tgcaactttt gttggctcag atgtgccgcc ttcttgcaac tgcttgccaa
    22861 tctcatcaag agtcatatta ggcgctaact gataataacc agcttgaaag ccagttaagt
    22921 ttttaaattt tgtataataa ttgaaaacaa tcccactttt aataatattg tctttctcca
    22981 aaatttcgcc aatctgctta ttagaagagc cgtttgggat ttccacttgg actaattggt
    23041 cgtccgattt atctaaaggc ttcaacccgc tatctacata acgatatacg gtaaacccga
    23101 gaaatccgcc aataatcaat aatgtaagag cgatcactaa aatgatcttc ctcacgatct
    23161 tatcttcttt tttacgcaga tttctccgct tgtcgattat atctgctgta gctgccttac
    23221 tggcttcttt tttgtctttg cttgggtgtt gtttgtttcc tgacaaaaca actcctcctt
    23281 actatcatct tcgtaactct gtttattata catgaaattt ctactgccgt gaattatttt
    23341 accaaatatt atgtcaaaat tgtaagtggc aaatgctctt tttgaggatt acaatgaaaa
    23401 atagttaaaa atggatagaa aggatttaat atggcaaaag gaaaacatca aaaaaaacag
    23461 cagatctctc tttcttttct cgcagttttg ttgctggctg tcagcttctt ttcaacaggc
    23521 atcattacag gatggcaatt aacaaaacaa cctcaaccac aaatttataa aacggaaccc
    23581 gcacaacttg cttccaattt aacatctaat gagcagatgc ttgccagcaa aattgatcaa
    23641 ttactgcaaa aatcagatta tatcggttcg atttatgtac gcaaaaataa tcgagtgata
    23701 ttagaaaaag gatatgggta cgcaaatcta cgcataaaag aatcgaattc tccttctctt
    23761 tactatcaaa tcggttcgat ccaaaaagcg atgactgctt tacttatcct aaaacaaatt
    23821 caatctggca agataacttt tgacacaaag ctatctgaat tttatcctca gattcctgga
    23881 agtcagcaaa tctcaatcaa aaatatgctt tacatgcgct ctggtcttca tcgaaccgct
    23941 tctccaaaaa aacctatgtc agatagtgag atcattcagt ttgcccttca tcatttgaaa
    24001 tttactggtt atgatacgta tcattatgaa ccattaaatt atacattgct tacaggggta
    24061 ttgatcaaac taatgaatca gccttatgaa actcttttaa aaaaagaaat cattcggcca
    24121 ctccatctag aacatacaga tttttatgaa aatgtaaaaa attcccctca gcatgctgta
    24181 tcttaccaaa tgtctgcaac tgacgattat tcaaaagcat tggtcgagcc tgaaacagac
    24241 atccgaaatg aattaggcac aggaaatata agtatgtctg tttatgactt gaacaaattc
    24301 tttaccagtg ttctgtctgg cgatattata ccaaaagaac tacttttctc attgtggcaa
    24361 aataatggtg ataaacatcc ttacagcggc ggtgtttaca gtggaaatga ttatatcctt
    24421 gctcagggaa atatcaatcg attccatgca gtagcggcga tgaaaaaaga cacaaaagat
    24481 gcaatcgtca tggaaagtaa tatccaatct gataaaaaca tcaaattgcc ggccactgat
    24541 ttaagaaatc agatttacga actgatggaa ggtgttaatt taaaaagcta agtcgagaca
    24601 aaaaaaccag caggatgctg gttttttgtt tcaatcgatt tctgtcttta aataacgttc
    24661 cattccgttc aatatatcaa atttgggttg ataacctaaa gaacggatct ttgaaatatc
    24721 cgctaaagaa tcacgaatat cccctgaacg ctccgcttca tatttaagcg ctagctctgt
    24781 tcctaagatt tgatcaattg aatgaatcaa ttcaagtagc gtagtgcttt ttcctgtacc
    24841 gacattgaat tgctgtccta aagctttttc ttccttagca acaagtaaaa gtgcttgtat
    24901 aacatcttct atataaacga agtctctaga ttggctacca tcaccaaata aagtaaattc
    24961 tgttttttct cctgccaatt gttttttgta tcggtccact agaatcgaaa taactccgct
    25021 atatggagag ttaggatttt gatttggacc ataaacgtta aaaaatcgga cagcactagt
    25081 cggaacatca tataaatgac agtaatccaa aacatattgt tctgatgcaa acttatcgat
    25141 ggcatatggt gttaaaggac ggatgaccga ttcttctttt tttggcaacg ttggttcatc
    25201 gccataaact gcagctgatg aactaaatac caatcgtttt aaaccagatt gatattttcg
    25261 taccaattca agcaataaaa gcgcactgtc aaaatttaca cgatgagttt ctagaggacg
    25321 ttctacagaa tctgccacac tagctacagc agctaaatga aaaatatagg caaactgata
    25381 ctgtttcatg attctttcca tcaattgagg atctgataca tccccctcaa tgaaagtcac
    25441 ttgtttctcc atatccaagt tttctttttt tcccattgat aaatcgtcga ctactacgat
    25501 tttgttttct tttcccaaat aattcgctag cgttgaaccg ataaaaccag ctccaccagt
    25561 aatcaatata gtatccatca aattcactcc aattatgcat tctcatactt aataaagtta
    25621 gtttaccatt tttatattgt aaaaactata aaatttgttg aagttgggat aactagtttt
    25681 cttttctact ttatatacct aaaaatagtt tcaattagtt actccttctt ctttagatat
    25741 agttttttgg atagaaccca gatatcccca aaaaataaat gaacctatcg tactaaccaa
    25801 aatgagttct gtgataaata atgccgaaac tgctattgta agaatcatta aaatagcaaa
    25861 aaagtgctta tcttttacta gatttggttg tgtgaaacaa taacgcaaag ttttaaagca
    25921 ttggataaat aagaaggtta acaatgtcaa tagacctaat cctcctgtag cagccataac
    25981 taagaaaata aaattatgag cagttaaagt atcttttccc attaatgtat atggtagttt
    26041 atcatgtgcg tattcaatat agtatcctgg agaaccgccg gatatcggct tactcttgaa
    26101 tatttcaatt gcacttttcc aaagtccaaa ccttaaatta gaaacatccc cgccatcagt
    26161 gtcagggcgt tttaaatcaa ctttattgct gctagttgtt cctgatgaat cagatatatc
    26221 tttttttatt tctgtaatat gcggaacatg tgcccatgct tgagcagtta tatctgttac
    26281 ataatacatc gcaattatac ttagcaatcc tactaccaat gcagttagcc atctttttaa
    26341 agtattagtt ttaagaaaac gactactata acttgtaaag aaactaaaaa caaaagttgc
    26401 tattaataaa caaactaatg tggtgcgtga gcccgataaa gcaataaacg agaattgaat
    26461 aaaaatatta attgcaaaaa atattttaaa tagaagattt aaatgcttgt aagtgaccaa
    26521 ataatatata gcaataatga tgacaattaa tgatataacc gcaccaaaat ttggatctgt
    26581 atatacacca aataaacggt tgcctaacca ccctaaacga actgggtaat acttatattt
    26641 tattggaatt tttatgccat attgaatgac aaaccagtat aatgatacta aattagctaa
    26701 aaaccatacc aatgaaagta tccaagtaaa tactttgaaa aaaaagttac tactgtagtc
    26761 tttgccaaac tgataaatca taaagaaaaa gattaattgc cacatgatta acttgatatt
    26821 ttcgctatat ccatatttta aattcattaa actgcttact agcatcgcgc caataaaagc
    26881 aattaatagc caatcatatt ttttaagatt tttttctcta aatgcaataa ttgcatctat
    26941 aatgatcaag acaactcccg ctattccagc aggccaaaaa agtaaagtat catatctagt
    27001 aggtaatagt aaatacgggc ctattgctcg taacattaga ataaaaccaa aataaataaa
    27061 ataccaacct ttcacatatt ttaaaaattt ttcattcaac atttttcctc catccaccta
    27121 tttctcataa acatttctta tcctttttag gataaactgg ttctttcaat tctaacaaaa
    27181 aaatgctaaa ctaacaatgt atattaatga atttcgagag gtgaaagatg aaaagagtaa
    27241 ttacttacgg tacctttgat ttgcttcact acgggcatat caatttactg aaacgagcaa
    27301 aacaatatgg agattatctt attgttgccc tttctacaga tgaattcaat tggaatgcga
    27361 aacaaaaaaa atgttatttt agttatgaaa aaagaaaaca gttacttgaa gctattcgct
    27421 acgttgattt agttattcct gaagaaaatt gggaacaaaa agtaaaagat ataaaagaat
    27481 accatgcaga ttatttcgtg atgggcgatg attgggctgg acaatttgat gaacttgaac
    27541 aactaacaga tgcacaagta atttatttac caagaacacc tgagatctct acaactcaaa
    27601 taaagaacga acttaattct ttaaatagtg ataaataata ttttttaaac aatgaaagaa
    27661 caatatatct ggataagacg taaaaaaatg gctctataat ttctaggact gatagaattt
    27721 tctgtcgatc ctagattttc ttgtaaaaaa aacagacata caaaaatttc gataaaatag
    27781 agtcgtctaa aacatcaatc tgaaaggaag attttgtata tccatttctc gttctatcaa
    27841 agagcacttc actattaaag ataaaaatat tgaaattaat tcttttcaaa aaacttttta
    27901 taaggacaag aaagccatcg taatcgaagg tacattagtt cgcactttta gacattgtct
    27961 attttgtaaa gaaactgcga agcagctcgt gaaaaacggc aagaagattt caatgattat
    28021 cttaaatcgc agtgggaata aataaaccta tttgcgtctt agaaaacaac attaccacta
    28081 ccaatcatgc ggaaaatatt ttactgctca tacgtatctt gtcgttcctt catgttttat
    28141 ttcgaaacaa gttcattaca cgatattaga agagttgacc gagcgtcaag cgttaaagac
    28201 acttacgaaa cgttgtgatc tttcagtaac gacatttcaa cggacattat ctgctctatc
    28261 ggcaatgctg cgacagatgc tagactattt acctcattgt ttactattcg acgagtttcg
    28321 ctctctttca atttcccatg gaaagttctc cttttcttgt atgaatagag agacagggaa
    28381 gctctttgac attttgtcca gccggagaaa aaaagatttg attgcttatt tcatgcgttt
    28441 tacaagaaaa gctcatatgg gggttcgata cattgtcaca aatatgaatg ccccttattt
    28501 ttcattagtc aaagagtcct ttccaaacgc ccaaatcatc attgattgct ttcacattgg
    28561 acaacacttg aacaagtttt tcgatcgtgt tcgaaaacgt gtgatgaagc agttgaatca
    28621 aaaagacact tcacaagcta agtactatcg acaattgaaa cctttgtata aattacttct
    28681 aaaatcagat gacgagttgg attacacaac attcaagaaa tggcagaatt ttcaatggcg
    28741 ctatttaaca gaaagtgaag tagttgatcg tctaccctca atctcttatg aacttaaaat
    28801 tgctaagcag tttatcaaaa gctgcttagc aattatcaca agaaacagct agctacttcg
    28861 agaaacgacc aaagaagttc cctcgaaact tcactatctc aaaaagcctt tttcatatct
    28921 gaaaaaggct tttaactagc attttcgaaa aagtattcta ataacaaaat tagagaatct
    28981 tcgcacacat atcaaaacat tgaaacgatt tgcttatggg tttcgcagtt ttaccaatat
    29041 gcgcttgtgg atttttctaa tcaacaggtt aatcagctat gcattaaaag ctagtaactc
    29101 ttactaactt tgttgttcat aaatgacctt atcagcccga tttgacaaag aa
//
LOCUS       DACGLE010000029        29109 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0534
            SAMEA3304449-rid9560453.denovo.030, whole genome shotgun sequence.
ACCESSION   DACGLE010000029 DACGLE010000000
VERSION     DACGLE010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304449
            Sequence Read Archive: ERR985064
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 29109)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29109)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702225.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702225.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 20:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,045
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 27 of 67
            Pseudo Genes (incomplete)         :: 25 of 67
            Pseudo Genes (internal stop)      :: 19 of 67
            Pseudo Genes (multiple problems)  :: 4 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29109
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304449-rid9560453.denovo.030"
                     /strain="VREN0534"
                     /db_xref="taxon:1352"
     gene            complement(<1..1030)
                     /locus_tag="IXT20_001401"
     CDS             complement(<1..1030)
                     /locus_tag="IXT20_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ4859855.1"
                     /translation="MKSNEKTPLTTSLILLLSITCGVVVANMYYIQPIGTKVAASLSV
                     STSAVGILTMLTQLGYALGLLFLVPLGDVVDRPKLIIRMVALSAVSLLAAFFAPSFTL
                     FACASFLIGLLSIVPQIIIPYGAVLAGPKARGKVMGTLLSGLLVGILLSRTVSGIVAS
                     LFSWRMIYLFALCAVGILTLLLYWKMPRSVQQSSTAVSYLDSLKSLPHLVASQRLLRE
                     AAVSGFFMFGTFSIFWSTLIFYISSPAYNWGTFEAGILAIFGLSGAVAAPIVGRLADS
                     YSERKIVYMGLVMQTISFIALLVGGSHLAILLLAIILLDVGNQFGQVANQTRVQGLGE
                     AASNRNNTV"
     gene            complement(1112..1801)
                     /locus_tag="IXT20_001402"
     CDS             complement(1112..1801)
                     /locus_tag="IXT20_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HAQ4859856.1"
                     /translation="MKKKIIRLMLISLGIILILVVGYRLYLEYQTDIQLFLNPRASKA
                     ELLAHIRSHGTFASILLIVLTAVMCAVPGIPTSVVGVIAGLSFGPLTGAIINILGNAL
                     GNITAIFLMHRLKFLDKKTETNHWVQAIRQMKHPKIGVMLGYMIPVIPSSMVNFAADA
                     MKLPLQQIILSIVIGVIPSSVLYACGGEALFHGYNKTAVTLVASVTVLVGLVVFIYKD
                     RKKKQGKQKHK"
     gene            complement(1907..2275)
                     /gene="rplL"
                     /locus_tag="IXT20_001403"
     CDS             complement(1907..2275)
                     /gene="rplL"
                     /locus_tag="IXT20_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HAQ4859857.1"
                     /translation="MALNIENIVAELKEATILELNDLVKAIEEEFGVSAAAPVAVAAA
                     GGAAAAEEQTEFTVELTAAGDQKVKVIKAVREATGLGLKEAKAVVDGAPAPVKEGVSK
                     EEAEELKAKLEEVGASVTVK"
     gene            complement(2328..2831)
                     /locus_tag="IXT20_001404"
     CDS             complement(2328..2831)
                     /locus_tag="IXT20_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HAQ4859858.1"
                     /translation="MSEAIIAKKAAIVDEVSEKFTAAASVVVVDYRGLTVDEVTRLRK
                     QLRDANVEMKVIKNSILSRAAKKAGLEGMDEVFTGPTAVAFSNEDVVAPAKIMDEFAK
                     EAKALEIKGGIIEGKVSSLEEITALAKLPNREGLLSMLLSVLQAPVRNVAYAVKAVAD
                     KEDGDAA"
     gene            complement(3241..3930)
                     /gene="rplA"
                     /locus_tag="IXT20_001405"
     CDS             complement(3241..3930)
                     /gene="rplA"
                     /locus_tag="IXT20_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HAQ4859859.1"
                     /translation="MAKKSKKMQDALKKVDSSKVYPVAEAVALAKETNIAKFDATVEV
                     AYRLNVDPKKADQQIRGAVVLPNGTGKTQTVLVFAKGDKAKEAEAAGADFVGDDDMVQ
                     KIQNGWFDFDVVVATPDMMATVGRLGRVLGPKGLMPNPKTGTVTMDVTKAVNEVKAGK
                     VTYRVDKAGNIHVPIGKVSFDDEKLVENFAAIHDVLLKAKPSAAKGQYMKNVTITTTF
                     GPGIHVDQASF"
     gene            complement(4034..4456)
                     /gene="rplK"
                     /locus_tag="IXT20_001406"
     CDS             complement(4034..4456)
                     /gene="rplK"
                     /locus_tag="IXT20_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HAQ4859860.1"
                     /translation="MAKKVEKIVKLQIPAGKATPAPPVGPALGQAGINIMGFTKEFNA
                     RTADQAGLIIPVVISVYEDRSFTFVTKTPPAAVLLKKAAKIEKGSGEPNKNKVAKVSS
                     DQVREIAELKMEDLNAANVEAAMRMVEGTARSMGITVE"
     gene            4799..5467
                     /gene="sdaAB"
                     /locus_tag="IXT20_001407"
     CDS             4799..5467
                     /gene="sdaAB"
                     /locus_tag="IXT20_001407"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit beta"
                     /protein_id="HAQ4859861.1"
                     /translation="MKDLRYRSVFDIIGPVMIGPSSSHTAGAARIGKIIRSIFGEQPD
                     SVDIYLYESFAKTYRGHGTDIALVGGLLGMEPDDERLADSLKLAYEAGMEVLFVPKSE
                     KAEHPNSVKMLLKKGTRKLSVTGISIGGGNIQISELNGFKISLSMGTPTLIVVHQDVP
                     GMIAKVTSILSETNTNIGTMTVTRESKGENAIMIIEVDDPQVEETVKRLKQLPNIDSV
                     NYFD"
     gene            5480..6352
                     /gene="sdaAA"
                     /locus_tag="IXT20_001408"
     CDS             5480..6352
                     /gene="sdaAA"
                     /locus_tag="IXT20_001408"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HAQ4859862.1"
                     /translation="MFLSIEELVDQAKAYPSVAELMIAVEMEMSGRSRSHIIEIMEKN
                     LSVMEQSINEGKEGVTSVTGITGGDAKKLDDYLNEADFLSGETILKAVRNAVAVNEVN
                     AKMGLICATPTAGSAGVVPGVLMAVRDRLDLTHEQQLEFLFTAGAFGLVIANNASISG
                     AEGGCQAEVGSASAMAAAALVCAKNGTPHQAAQAVAITLKNMMGLICDPVAGLVEVPC
                     VKRNALGSSQAFISADMALAGIESVIPPDEVVAAMYQVGRQMPSIFKETAEGGLAVTP
                     TAKRLAEEILNKRA"
     gene            complement(6443..7462)
                     /locus_tag="IXT20_001409"
     CDS             complement(6443..7462)
                     /locus_tag="IXT20_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate oxidase"
                     /protein_id="HAQ4859863.1"
                     /translation="MEAGAFGYIRGGSEDEWTMKENTTSFNTKKIMPRILRGIDSADL
                     HTSVFGIELDTPIIQAPSAAQGLAHEKGEADTAKGVAAAGSIFSISTYANTTIKDAAD
                     AAPGAPQFFQLYMSKDDRFNEFILNKAVEAGAKAIILTADSTLGGYREEDVINQFQFP
                     LPMPNLAAYSEQSASGNGEGKGIAEIYAAAKQGLTPDDIKTIKEITHLPVIVKGIQSP
                     EDAVIAISAGADGIWVSNHGGRQLDGGPASFEVLPKIAEVVNKRVPVIFDSGVRRGEH
                     VFKALASGADLVAIGRPVIYGLNLGGAEGVTSVFEHLNKELSITMQLAGTKTIDEVKN
                     TKLMD"
     gene            complement(7635..8522)
                     /locus_tag="IXT20_001410"
     CDS             complement(7635..8522)
                     /locus_tag="IXT20_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HAQ4859864.1"
                     /translation="MTEPIKNLCKRATAAALVLISLFALSGCQTTKINTTEVSDKEKT
                     AESSKVIEKTTIPTLFFHGYSGTENSFKGMLRRLEKNQQAVKELVLTVGTDGQIQATG
                     DLSGKADNPMVQVLFEDNKNNEWNQAEWIKTCLLYLKENYGIDKVNIVGHSMGGVSAL
                     RYLGTYGQDTSLPQVQTLTAIGAPFNDFVDDSAQSLTDELAKGPAVVSNRYQDYQQMI
                     GNIPITTRFFLIAGQLDETDLSDGTVPLNSALAVYALLKQRGNEIEEKVVTGENASHS
                     MLHENQEVDQLVSRFIWKE"
     gene            complement(8599..9144)
                     /gene="nusG"
                     /locus_tag="IXT20_001411"
     CDS             complement(8599..9144)
                     /gene="nusG"
                     /locus_tag="IXT20_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HAQ4859865.1"
                     /translation="MESFEKNWYVLHTYSGYENKVKANIESRAQSMKMADFIFRVVVP
                     EEKETEVKNGKEKEIVHKTFPGYVLVEMIMTDASWYVVRNTPGVTGFVGSHGAGSKPA
                     PLLPEEVNHILRSIGMSLRQNDLEVEVGEVVKIIEGAFAGLEGQVTEVDGEKEKLKVN
                     IDMFGRETSTELDFDQVDKID"
     gene            complement(9245..9415)
                     /gene="secE"
                     /locus_tag="IXT20_001412"
     CDS             complement(9245..9415)
                     /gene="secE"
                     /locus_tag="IXT20_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HAQ4859866.1"
                     /translation="MKFLKSVKDEIKQVTWPSRKQLRKDTLVVIETSIIFGLMFYVMD
                     TAIQSLFGWILK"
     gene            complement(9439..9591)
                     /gene="rpmG"
                     /locus_tag="IXT20_001413"
     CDS             complement(9439..9591)
                     /gene="rpmG"
                     /locus_tag="IXT20_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HAQ4859867.1"
                     /translation="MAKKKAALACSVCGSRNYTKSVSEGKSGERLEINKFCKYCNQYT
                     LHKETK"
     gene            9753..10397
                     /locus_tag="IXT20_001414"
     CDS             9753..10397
                     /locus_tag="IXT20_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HAQ4859868.1"
                     /translation="MLLKTLVYKKKDLTTVTETSTLEEALKILETSGYRCVPILDSSG
                     NIFRGNIYKMHIYRHKANGGDMTLPVTHLLKNATKFIYLDTSFFKVFFTIKELPYIAV
                     LDSENHFYGILTHSTLLNMLSQSWSVEQGSYVLTIASTGKQGDLAAIAKIISKHSSIA
                     SCITLDIEKDEYIRRTLITLPANTEAKVCETIVNHLEKKNYKVVEIENLQNQEA"
     gene            complement(10453..10956)
                     /locus_tag="IXT20_001415"
     CDS             complement(10453..10956)
                     /locus_tag="IXT20_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HAQ4859869.1"
                     /translation="MQKTKSRQRLSVIVVNGIIMALYLALTILVAPVSSGPIQFRISE
                     SLNHLVVFNRKLLWGVLGGVIVYNAFFGFGIMDVVFGGGQTLLSLGLTALLQNKVKNV
                     KMRLVLNTIFFTISMGLIAYMLVPTGGQAFWTTYGTLALSEFIVMAVSAPLMYYLDRA
                     LDFEKRV"
     regulatory      complement(10969..11015)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            11553..12518
                     /locus_tag="IXT20_001416"
     CDS             11553..12518
                     /locus_tag="IXT20_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HAQ4859870.1"
                     /translation="MKFHYHILINPSAGSGNGYKVAERILPVLKNKHIDYTLHYSEYK
                     GHDAEIAETLAKETLIPWIEEEQKTIDTFPLLIIVGGDGTLHQVLDTFYQMEVEFPVA
                     YIPAGSGNDFARGAGLPKNPKKGLQLILAAQSPEKVHIMAYEEKISEKKGIAVNNFGI
                     GLDAAIVHATNHSSTKKRLNKYNLGSFSYLFSILRALFTQKGFPILVDAGGKQLSFSN
                     AFLCTATKHPFFGGGIAIVPTADASKPVIDFVVVERINFFKILWLILLLIQKKQLKSK
                     YFHHYTTNRLRIVSTTPQHGQEDGEEMEKRPFDITITTTKQLFWC"
     gene            complement(12617..13789)
                     /locus_tag="IXT20_001417"
     CDS             complement(12617..13789)
                     /locus_tag="IXT20_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ4859871.1"
                     /translation="MTLYKTIWTLLKANKSNLLIGVIITVVATFFFAQNIDVENTSLQ
                     DAKIALISEEESPVVDGLRKYLNDKETVVTLDGTSQKEIDDALYFNRVNIILHLPADF
                     TEQVEVGKMATIKIQSRPDAFSKTVVTQQVNTFLQTLILFQEEDKTIDAAMEQTQMAL
                     SVTGEVELSAGYSQRMKKLLTGTTFNFLSYGLFLSIFSGFSVINLAFNRKEISKRNQS
                     APITKRNLNRKVTFSLISYSLLLFSGFLLLMVLLVIHTSWDITVWYHILNTFLFLLPI
                     ISFSACITSLVKNSETSGGIKDIFITGSCFIGGVFVPAEYLPEMVSKIAAFTPTYWFV
                     QNNNLISETLSYNQTFAESFWFNGCVLVAFAAVFSMIQFILGKERNYRWQPNWAKN"
     gene            complement(13776..14921)
                     /locus_tag="IXT20_001418"
     CDS             complement(13776..14921)
                     /locus_tag="IXT20_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ4859872.1"
                     /translation="MFWHFYKYRTKILLRNKSMLFWTLAFPILLGLMFMAAFGEIDQG
                     GNLETINTGIVIKADQTALSENFTTVLEQVEVNDKPLFEVQELSEVEANEKLNADDLA
                     GFYLIDGSSVSLHVGQAGMSQTLMKNVMDQFLQRIGIISSKVAEVENVDLAPIMTVFE
                     QEVSFQEVHAGRNMSFKSFYFFTLIGMAILYGTMWGVRNVQDQQANQSSNGIRLSLIP
                     RKQSLVSIANLAASFTIVLTEIYIMLAVFRFIYQVDFGERWQWLMIVTALGSLCAILL
                     GTLVGNLIPKMNLVQKDSILISVTIAMSFFAGMMGSEQVKYWIDLHIPIVGQLNLVNL
                     ISESLYKLYFYTDLASFYTNLLWLIGFIFLFALGNAWMERGVRYDAL"
     gene            complement(14935..15867)
                     /locus_tag="IXT20_001419"
     CDS             complement(14935..15867)
                     /locus_tag="IXT20_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ4859873.1"
                     /translation="MIIEIDKLVKRYGDFVALNHFDLQVQEGEVLGLLGPNGSGKSTA
                     INCMLSLLNYDKGTIKLFGTTMTPENYAVKAMIGVVPQDVAVFDELSVQENIDYFCGL
                     YVSEKEKRKALIEEVIQLVGLEKYVSFRPGELSGGLKRRLNIACGIAHKPRLIFLDEP
                     TVAVDPQSRNKILESIKELNRQGATIVYTTHYMEEVEILCDQIVIMDQGQVIAKGTKE
                     SLKDMVRTADRLTIDIPLLPENVLSAIQRTIASDGIAYKKGQLQVTTTELNTVLLPIL
                     TILQEEKISYGKIATKEASLNDVFLEITGKDLRD"
     gene            15984..17063
                     /locus_tag="IXT20_001420"
     CDS             15984..17063
                     /locus_tag="IXT20_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HAQ4859874.1"
                     /translation="MHKSRLLNETSFLLVISLLLLVSQGFVAMQVIYLLIAVLFTGVS
                     YLLPTKPNWIFQVILFLLCAFLPDLVLFLPATMRVIAPEESHLDYLFLLGFLLISTVG
                     NHPPIKVAFVVILLFFSFYLRKKDQRTTDLEKKYIVSKDTHWEKQQELSEQNKALALS
                     QETQLALQIVEERNRIARDIHDNVGHLLSSAILQIGALEMINQEPKLKEPLENLSATV
                     HTGMDRIRQSVHDLHETSISFQHSLEFLIADFPSSVTIEGNLFEGLNETQQNCFLMVI
                     KEALTNSIKHSHAKTVVLHFRTLPAFYRLQITNDGTSPQKKSAPGIGLKTMRQRVQEQ
                     KGQVHIFQENQQFQLTIILPREGHQ"
     gene            17060..17689
                     /locus_tag="IXT20_001421"
     CDS             17060..17689
                     /locus_tag="IXT20_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ4859875.1"
                     /translation="MITVVIIDDDPFVTKSLQTILESTKEIRVLGIGHCAKDALVLYE
                     THRPDVLLTDIRMPKQTGIDAAKEILAKFPKAILLLLTTFKDEEYIREAFSIGVKGYL
                     IKQNLQAIIPSVKSAYNGQAVFGNEIIETFTQMMKNKPTIDSHAYASFSERELAIIKE
                     VAAGKNNKEIADALYLSDGTVRNYISQLLEKLDLRDRTQLAIYFYQHLQ"
     gene            complement(17799..18026)
                     /locus_tag="IXT20_001422"
     CDS             complement(17799..18026)
                     /locus_tag="IXT20_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005229738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAQ4859876.1"
                     /translation="MAKETEDKLAKLAKESGNDFKKLDEMEKEFKLNESPDVERDDRE
                     ESKLEKTLDKAPIFPPNTPNNPTNPGSPANP"
     gene            complement(18129..20717)
                     /locus_tag="IXT20_001423"
     CDS             complement(18129..20717)
                     /locus_tag="IXT20_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HAQ4859877.1"
                     /translation="MQPEEMPYFVGTVAAIFFQNPSNFYKVLLVHISETTADYREKEI
                     VVTGSFGDIQENETYRFYGELVDHPKYGRQLKLERYEQEKPTTANGIVAYLSSEKFPG
                     IGKKTAEKIVELLGEDAIEKIIADPSVLKQISGLTKPKREMLAETIRLNHGMDQVIMG
                     LNRYGFGSQLAFSIYQAYKNESLDIIEENPYQLVEDIEGIGFKRADNLAEQIGIAADS
                     PRRIRAAILHQIFQHAVQTGDTYVPAETLLQQTIRILETSRPIEIQPDQVATVIIQLV
                     EEGKIQQEETNLYENSLYFSEWGIGNSIQRLLSRKKEIHYPEKEVQKNIRRIEKIFGI
                     QYGPSQEQAIAEAIRSPLFILTGGPGTGKTTVINGIVNLFAELNGLELDPSKYTQEIF
                     PILLAAPTGRAAKRMNETTGLPASTIHRLLGLNGREKTPSVSTKELEGGLLIVDEMSM
                     VDTWLANTLFKAIPTNMQVILVGDKDQLPSVGPGQVLHDLLEIEQIPKMELNQIYRQG
                     DGSSIIPLAHEIKEGRLPKDFTKNQKDRSYFRSDAYQIEPLISKIVEKAKNKGFRAQD
                     IQVLAPMYRGPAGIDALNKMMQEIFNPGKPGKKEVHWNDTVYRIGDKVLQLVNTPELN
                     VFNGDMGEITGIILAKDSEDKVDELVIQFDANEVTYKRNEWNKITLSYCCSIHKSQGS
                     EFKMVILPMVHQYQRMLQRNLLYTAVTRSKELLILLGEEQAYVTCVANESASRMTTLK
                     SRIMDNDKMNITLRTKLAAYEDSLVGNDPFEDGNYTKEPRVVKEKLPVEDDSGTNETK
                     NQVMETVEPDLFSSFVGEEKKEEVEPAVSKDFVLTAEVIDNQLVDPMIGMENSSPYDF
                     IPAKNA"
     gene            complement(20737..21396)
                     /locus_tag="IXT20_001424"
     CDS             complement(20737..21396)
                     /locus_tag="IXT20_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HAQ4859878.1"
                     /translation="MELYFTRHGKTQWNQERRFQGSNGDSPLLPQSYEEIKAFGKKVK
                     YVPFKAIYSSTAKRARDTAEGINKELARPVEIFYTDKLRELGLGTLEGQSIDEMYQKY
                     PDNLPNLRNHLDLYDPSPFHGETIESAITRIETVVADAVARHKDGPLLFVGHGASLTA
                     AIQWMTGKELSELREMGGLFNSSLTILETEEPCRLLPYELKVWNQTDFLGTETAPEPL
                     L"
     gene            complement(21445..21948)
                     /gene="trmL"
                     /locus_tag="IXT20_001425"
     CDS             complement(21445..21948)
                     /gene="trmL"
                     /locus_tag="IXT20_001425"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HAQ4859879.1"
                     /translation="MNHIVLFEPLIPANTGNIARTCAATNTPLHLIEPLGFSTDDKHL
                     KRAGLDYWNDVNITYHSNLEAFLTFLGNRKLHLISKFAHKVYSDENFADGEEHFFLFG
                     KETTGLPETFMRENEEKCLRIPMNDEHVRSLNLSNTAAMIVYEALRQQGFPNLELTHH
                     YENDKLD"
     gene            complement(22169..23035)
                     /locus_tag="IXT20_001426"
     CDS             complement(22169..23035)
                     /locus_tag="IXT20_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HAQ4859880.1"
                     /translation="MLKKIDRGRQFLAENHQLFRCPVCQEEVKKSDNGLICKNNHRFD
                     LSKKGTLYFLSHAVKTEYDQGMFVPRRRMIQSGMYQPVIETIAQHLPEEVSLLDVGCG
                     EGSFLNELSKLKKIQNAVGFDISKEGVYAATEQSAEAFWCVADLTNLPFADQTFNAVL
                     NIFSPSHYQEFQRVLSEDGEVLKVVPQENYLKELRQAFYPNQPEKQSYSNERVVAKFA
                     EAMKLTERQRVTYEFEIPEENQKDLLAMSPLEWQVEERIKADLYKNPLKKITIDIELL
                     KGTKRKRFSSSF"
     gene            23144..23785
                     /locus_tag="IXT20_001427"
     CDS             23144..23785
                     /locus_tag="IXT20_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HAQ4859881.1"
                     /translation="MIKEFCAENYTSIPLAIARGADRIELCDNLAVGGTTPSTGVIEE
                     TLSYAGEKDIPVMTIIRPRGGDFVYNDIELKIMHTDLIEAKKLGTDGVVFGCLTPSGW
                     LDEEALELLIETAEGLQITFHMAFDSIPEDRQFEAIDWLVEHGVDRILTHGGPADKTI
                     VENFPRLKELIAYTDGRLIILPGGGVTAENAAHVAKELNVSEVHGTKIVDLQL"
     gene            complement(23847..25208)
                     /gene="mgtE"
                     /locus_tag="IXT20_001428"
     CDS             complement(23847..25208)
                     /gene="mgtE"
                     /locus_tag="IXT20_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HAQ4859882.1"
                     /translation="MDENQELEERFAQLTQDLRENNIQAFRNSFLEMHIYEQGQFYQS
                     LTEENRQLIYSYLSPKELADMFDVIEEDNEHMKEYLDEMRPSYAADMLSEMYTDNAVD
                     LLNTLDKKQIAKYLSLMSADDASEIKELLHYEDETAGAIMTTEFVSIVANQTVRSAMY
                     VLKNEADVAETIYYIYVVNQEGQLVGVISLRDLIVNDDDAMISDLMSERVLSVHVGDD
                     QEDVAQTFRDYDFLALPVTDYDDHLLGIVTVDDIIDVIDDEAASDYSGLAGVNVEEIN
                     ENPVKAASRRLPWLITLLFLGMSTASLISHYEDLVSEASILAVFISLITGTAGNAGTQ
                     SLAVAVRRLAISDEKDHGFVRLVLAEVLTGVVTGAVTGITIFLIVGFWKHNFILGFVI
                     GMAMLFAITVANLAGSLIPMLMDRLGFDPAVASGPFITTLSDLTSVLIYFNIASLFMG
                     YFM"
     gene            complement(25226..26125)
                     /locus_tag="IXT20_001429"
     CDS             complement(25226..26125)
                     /locus_tag="IXT20_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HAQ4859883.1"
                     /translation="MQFTWDYEKDHSQQVKYFLKEKGISKGLLAKIKFQGGQIKVNDQ
                     VENVLFSLAKDDKVTIVIPAEGEHETVLLDETPIDIVYEDEHVLVVNKPAGVSSIPAQ
                     YHPNGTMANRVKAYYKKQGYENQVIHVVTRLDRDTSGLMLFAKHGFAHAKLDMQLREK
                     KFVKKYQALVSGRLSDLSAHGHIDLPIARDYSSLLKRMVSDEGRSAQTEYWIEKRLTE
                     IALVDIQLHTGRTHQIRVHFSAIGCALLGDDMYGGRMDLGIKRQALHCYDLRFYHPFT
                     EELLEFKQPLAADMEAVVRRFEE"
     gene            complement(26127..26924)
                     /locus_tag="IXT20_001430"
     CDS             complement(26127..26924)
                     /locus_tag="IXT20_001430"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HAQ4859884.1"
                     /translation="MKVAIVHNKEAKTIEVTERLNALLEQAGIQRDDHEPELVISVGG
                     DGTLLSAFHHYSHCLNDVRFLGVHTGHLGFYTDWRDYELEELVDSLRTNREQSVSYPL
                     LDVRISYLDETPDQHFLALNESTIKRANRTMVADVYIKNELFESFRGDGLTISTPTGS
                     TAYNKSVGGAVIHPSINAFQLAEIASLNNRVFRTLGSPIVIAHDEWLEIKLENTEDYL
                     VTVDQLNVAKANIRSIYYRIAEERIHFASYRHMHFWHRVKDAFISED"
     gene            complement(26902..27606)
                     /locus_tag="IXT20_001431"
     CDS             complement(26902..27606)
                     /locus_tag="IXT20_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HAQ4859885.1"
                     /translation="MERDWEAFLAPYEQTVSELKVKLRGIRKQFREQNRHVPIEFVTG
                     RVKPVDSIVTKSQLRHIPMGRLEEEMSDIAGLRIMCQFVEDIHQVVEIIRKRKDMKVL
                     QERDYITNKKASGYRSYHLVIEYPVQLISGEKKILAEIQIRTLAMNFWATIEHSLNYK
                     YQGVFPEEMSERLQRAAEAAYQLDEEMSSIREEIQEAQRLFSHGRGKSDDVYYRTLLK
                     KESEREEAGDESSDRS"
     gene            27737..28312
                     /locus_tag="IXT20_001432"
     CDS             27737..28312
                     /locus_tag="IXT20_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HAQ4859886.1"
                     /translation="MTQMLEIEYKSMLTKQEYEIFIAHYQLTDKDFRVQTNIYFDTVD
                     EQLKKQNCGLRIRFVGHQAEYTLKTPAEKGRLETTDTFITEEAEAFIFEKRLPRSGAV
                     FQKLSDLNIDPASLIQTGKLTTKRAEFPIEEGLLAIDESWGDNLHDFELELEVGDASV
                     GKIAFINFLKRFSLPYRPAKNKIQRMLEAKN"
     gene            28430..29107
                     /locus_tag="IXT20_001433"
     CDS             28430..29107
                     /locus_tag="IXT20_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HAQ4859887.1"
                     /translation="MIEIYLFVNPLGEYCFEMEQQLLQFIEEEYGTPSKEKMQFRFLP
                     LVNLQTIGDVMQQKGISQNNLEERNHLFSMTYSAALDCKAAQFQGKKKGRRFLIKLQE
                     AVGCNDIPYSQELAESIFEEIGGDLNMFKEDRQSDFVKEVFFSDQKIAREMGITKHPS
                     AVVYNYACDRDFGVLVEVKGTSSLWRITELCKTTDNSYQVFNGEHLHTNDSQFHSRTS
                     HLHLLSN"
BASE COUNT         8566 a         6169 c         5232 g         9142 t
ORIGIN      
        1 atacagtatt atttcggttg cttgctgctt cacctagccc ttgtactcgt gtttgattgg
       61 cgacttgacc aaattgattc ccgacgtcta ataaaataat agctaaaagc aaaatggcca
      121 aatggcttcc gccaaccaat aaagcaataa agcttattgt ttgcataaca agtcccatat
      181 acacgatttt tctttcagaa taagaatctg ccagtcgtcc cacgataggt gcagcaactg
      241 ctccagacaa accaaaaatc gctaatatcc ctgcttcgaa tgttccccaa ttgtaggcgg
      301 gactgctgat ataaaaaatc aacgtagacc aaaagatcga aaatgttcca aacatgaaaa
      361 atccactgac tgccgcttct cttaatagac gttgagaagc aactaaatgc ggcaaacttt
      421 taagtgaatc aaggtatgaa acagctgtac tactttgctg gacagaacgc ggcattttcc
      481 agtaaagcag aagtgtaaga attccgactg cacataaagc aaataaatag atcatccgcc
      541 aagaaaaaag actggcaaca ataccagata ccgtccgaga aagcaaaatc cccacgagta
      601 aaccgctaag caatgtaccc atgaccttcc ctcttgcttt tggtcccgct agtaccgcac
      661 cgtaaggaat gatgatttgc ggcacgatcg aaagtaatcc tattaaaaaa gaagcgcatg
      721 caaataacgt aaacgaaggc gcaaaaaaag cggccaacag agagacggca gataacgcta
      781 ccattcgaat gatcaatttt ggacgatcca ccacgtcccc taaaggaact aaaaacagta
      841 accctaacgc atagcctaat tgggtcaaca tcgtcaatat cccgactgca cttgtgctaa
      901 cagataaact agctgctact tttgttccaa tcggctggat gtaatacata ttggcaacaa
      961 caacgccaca agtgattgat aatagtaaaa taagcgaagt tgttaaaggt gttttttcat
     1021 tactcttcat tgatttcccc catttcctat cttagtttac aacttatgga aaagattgga
     1081 aatagaaaaa tacaactatt cgccacactt attatttgtg cttttgtttt ccttgttttt
     1141 tcttgcgatc tttataaatg aagacaacta accctactag cacagtaaca cttgcaacca
     1201 acgtgactgc agttttgtta tatccgtgaa acagagcttc tccaccacac gcatacaaca
     1261 cagacgaagg gatgactcca atcacgatcg acaatatgat ttgctgtaga ggcaatttca
     1321 tcgcatctgc agcaaaattg accatagaag atggaataac cggtatcata tagcctaaca
     1381 ttacacctat ttttggatgt ttcatttgtc ggattgcttg cacccaatga ttcgtttcgg
     1441 ttttcttatc taaaaatttt aagcgatgca taaggaaaat tgcagtgatg ttaccaagag
     1501 cattacccaa aatgttgatg atcgcacctg tcagtggtcc aaagctcaaa ccagcaatga
     1561 ctccaactac tgaagtagga atacctggaa cagcacacat caccgctgtc aatacgatca
     1621 agagtatcga tgcaaaagtc ccatgcgaac gaatatgtgc gagtagctct gccttactcg
     1681 ctctcggatt taaaaataat tggatgtctg tctgatattc taaatacaac cgatagccca
     1741 ctaccagtat cagaatgatt cccaacgaga tcaacatcaa tcggattatt ttctttttca
     1801 tatttcctcc tgaaaaaaat ctatattcat taaataatag tttaacataa aaacaaaaaa
     1861 gcaccgaaat ccaacatagg atctcagtgc ttttaaatag taaaaattat ttaacagtta
     1921 cagatgcgcc aacttcttca agtttagctt tcaattcttc agcttcttct ttagatacgc
     1981 cttctttaac tggtgcagga gcgccatcaa ctacagcttt agcttctttc aagcctaaac
     2041 cagttgcttc acgaactgct ttgataactt taactttttg gtctccagct gcagttaatt
     2101 ctacagtgaa ttctgtttgt tcttcagcag cagcagcgcc accagcagca gctacagcta
     2161 caggagcagc agcagatacg ccaaattctt cttcaatagc tttaactagg tcgttaagtt
     2221 ctaggatagt tgcttctttt aactcagcaa cgatgttttc aatgttcaat gccattataa
     2281 tttcctccgt ttaattgttc ttttttattt aatgctttat gcggcaatta agctgcatct
     2341 ccatcttctt tgtctgccac tgctttgaca gcgtaagcca cgttgcggac tggcgcttgt
     2401 agtacagata gcaacataga tagaagtcct tcgcgatttg gcaattttgc caatgctgtg
     2461 atttcttcca atgaagagac tttaccttcg atgataccgc ctttgatttc taatgctttt
     2521 gcttctttag caaattcgtc catgattttc gctggtgcga ctacatcttc gttactgaat
     2581 gcaacagctg taggtccagt gaatacttcg tccatgcctt caaggccagc ttttttagct
     2641 gcacgtgaaa gaatagaatt tttgataact ttcatttcta cgttagcatc acgaagttgt
     2701 ttacgtaaac gtgttacttc atcaactgtt aatccgcggt agtcaacgac tactactgat
     2761 gcagctgctg taaatttttc gcttacttca tcaacgattg ctgctttttt tgcgataatt
     2821 gcttcactca tttttgagtt tcacctcctg aattttgatg gaagagtttt ttgcactgaa
     2881 aaggcaagtc acttttatag ttactttgct caaccagtat tattcatcac ctattcactt
     2941 cgttcatagt gattctactc gctttgctcg aaaggaaact cctttcgctt tgctcaacca
     3001 gtattgttca tcatctcttc acttcgttca tagtgattca caataaaaaa ctctatgcca
     3061 ccgcagacat agagggacta ttgaatatat tcaataaacg tcctcggtag gaaattaagg
     3121 cttgatgcca cctactgtct tcggtacaag ttaattatta acctcaacta gcttagcatg
     3181 ctaaactagt tgagtcaaga attatttttc agttggatca atcaacattc taatatttaa
     3241 ttagaaagaa gcttggtcaa catggatacc aggtccaaat gttgttgtga tcgttacatt
     3301 tttcatgtat tgacctttag ctgctgatgg tttagctttt agcaatacat catggattgc
     3361 tgcaaagttt tcaactaatt tttcatcgtc aaatgaaact tttccgattg gtacatggat
     3421 atttccagct ttgtccacac ggtaagttac tttaccagct ttcacttcgt ttactgcttt
     3481 tgttacgtcc attgtaactg taccagtttt agggtttggc attaagcctt ttggtcctaa
     3541 tactcgtcct agacgaccta cagtagccat catatcaggt gttgcaacaa ctacatcaaa
     3601 gtcaaaccat ccgttttgga ttttttgaac catgtcgtcg tcacctacga aatctgcacc
     3661 agctgcttca gcttcttttg ctttgtctcc tttagcaaaa actaaaacag tttgtgtttt
     3721 accagttccg tttggtaata caacagcacc acggatttgt tggtctgctt ttttagggtc
     3781 tacgttcaaa cggtatgcaa cttcaactgt tgcatcaaat tttgcaatgt ttgtttcttt
     3841 tgctaaagct actgcttcgg caacaggata aactttactt gaatcaactt tttttaatgc
     3901 atcttgcatt tttttgctct ttttagccat tttggttcct ccttgattgt ggttataacg
     3961 gttgtacctc ccacgtgttc tatatctttc aatatagagc caactgagaa gctgctgtct
     4021 tagggtgtat agattattct acagtgatcc ccatgcttcg tgcagttcct tcaaccatgc
     4081 gcattgctgc ttcaacgtta gctgcgttta ggtcttccat ttttaattca gcgatttctc
     4141 ttacttgatc gctagaaact ttcgcaactt tatttttgtt tggttcgcct gaaccttttt
     4201 cgattttagc tgcttttttc aatagaacag cagctggcgg tgtttttgta acgaatgtga
     4261 atgaacggtc ttcgtataca gaaataacaa ccggaatgat caaacctgct tgatcagctg
     4321 tacgagcgtt gaattctttt gtgaatccca tgatattgat acccgcttga cctagtgcag
     4381 gacctactgg tggagctgga gttgctttac ctgcaggaat ttgcaattta acgatttttt
     4441 ctactttctt tgccacgaga catacctcct taagtccgtg atgtggttaa ttggagatgc
     4501 atatttctcc tcccacgttt tatctgatct gaaaaaatcc agacaaataa tgtgcaaaac
     4561 aatgcacact ggtacagtat agcaaattta tttgtttttt caagattttt ttctgctatt
     4621 tttacatttt gcttgaataa acgctaaatt tattctaaaa aaacaacaat ttttcgttct
     4681 catttcttct tttatgttac acttgcgcta gcatggttca acagatgaaa gcaaacaata
     4741 acacggtttt ttagaccctt gtacaaagat tcctacataa caaaatgagg tggagaaaat
     4801 gaaagattta aggtatagaa gtgtctttga tattattgga cctgtgatga ttggtccaag
     4861 tagctctcac actgccggag ctgctagaat cgggaaaatc atacgtagta tatttggcga
     4921 acagcctgat tctgtagata tttatctata tgaatcattt gcaaaaacct atcgggggca
     4981 cggaacagat attgcattag ttggcggcct gttagggatg gaacccgatg atgaacgttt
     5041 agctgattct ttgaaattgg catatgaagc cggtatggaa gtcttgtttg ttcctaagag
     5101 tgaaaaagcc gaacatccaa actctgtaaa gatgcttcta aaaaaaggaa cacgaaagct
     5161 ttcagtcact ggcatttcga tcggcggtgg caatatccag atttctgaat tgaatggatt
     5221 caaaatttct ttaagcatgg gtactcctac cttgatcgtt gtccaccaag atgttccagg
     5281 tatgattgct aaagtgacta gtatactttc tgagaccaat acgaatatcg gcactatgac
     5341 agtgacacgg gaatcaaaag gtgaaaatgc cattatgata attgaagtag atgatcctca
     5401 agtagaagaa acggtaaaaa gactcaaaca gctgccaaat atcgacagcg taaattattt
     5461 cgattaagga gctaaaaaaa tgtttttatc gattgaagaa ttagtagatc aagcaaaagc
     5521 ttatccaagt gtagcagaat tgatgattgc agttgaaatg gaaatgagtg gccgttcccg
     5581 tagtcacatc atcgagatca tggaaaaaaa cctctcagta atggaacaat caatcaacga
     5641 gggaaaagaa ggtgttacct ccgtcacagg aattaccggt ggtgatgcaa aaaaattaga
     5701 tgattattta aatgaggcag actttttaag tggagaaaca atcttaaaag ctgtacgcaa
     5761 tgctgtagct gttaatgaag tcaatgcaaa aatggggttg atttgtgcta caccgactgc
     5821 cggaagcgcg ggtgttgttc ctggtgtgct gatggctgtc cgagatcggc ttgatttgac
     5881 acatgaacag caattggaat tcttatttac cgctggcgct ttcgggttag tcatcgcaaa
     5941 caacgcttct atcagtggtg cagaaggggg atgtcaagca gaagttggtt ctgcaagtgc
     6001 aatggcagca gcagctttgg tttgtgcaaa aaatgggact cctcatcaag cagcacaagc
     6061 agtagccatt acattgaaaa atatgatggg actgatttgc gatcccgttg caggcttagt
     6121 tgaagtcccg tgtgtcaaac gtaatgcttt aggctcttct caagctttta tctctgctga
     6181 tatggcatta gcgggtatcg aaagtgtcat tccgccggat gaagtcgttg ctgcaatgta
     6241 ccaagtcgga cgtcaaatgc cgtcgatttt caaagaaaca gcagaaggtg gcttagctgt
     6301 tacgccaact gctaaacgac ttgcggaaga gattttaaat aagagggcgt gataaagctc
     6361 cttaatttcg agtattaaga gattagttat cttcataaca aaaaacgaag agaggactaa
     6421 ttgcccttct cttcgttttt tattaatcca tcaatttcgt attttttact tcatcgatcg
     6481 tttttgttcc tgcaagttgc atcgtgatcg acaattcttt gttcaggtgt tcaaaaacag
     6541 atgttactcc ttctgcccca cctagattca acccataaat cactggtcgg ccaatcgcaa
     6601 ctaagtcagc accacttgcc agagctttga agacatgctc tcctcgacgg acaccagagt
     6661 cgaaaatcac tggtacacgt ttgttgacta cttcggcaat ttttggcaat acttcaaagg
     6721 acgcaggacc tccatctaat tgacgtccac catgattgga tacccagata ccatctgccc
     6781 ctgcagagat agcgattact gcatcttctg gcgattgtat tccttttacg ataactggaa
     6841 gatgggtgat ttctttgatt gttttgatgt catctggtgt caagccttgt tttgcagctg
     6901 cataaatttc tgcgattcct tttccttcgc cattaccact tgcactttgt tcgctatacg
     6961 cagctaagtt aggcattggt aatggaaatt ggaattgatt gatgacgtct tcttcacggt
     7021 aaccaccgag cgttgaatct gccgttaaaa tgatagcttt agcccctgct tctactgctt
     7081 tgttcaaaat gaactcattg aatctgtcgt ctttactcat atataattgg aaaaactgcg
     7141 gtgcgcctgg cgctgcatcg gctgcgtctt tgattgtcgt atttgcataa gtgctgatgg
     7201 aaaagattga tccagcagca gctactcctt tagctgtatc agcttcccct ttttcatgtg
     7261 ccaatccttg ggccgctgat ggcgcttgga taatcggtgt atctaattca attccgaaaa
     7321 cagatgtatg caaatcggct gagtcgattc cgcgtaatat ccgtggcatg attttctttg
     7381 tattaaaaga agtcgtattt tctttcatcg tccactcatc ttcagaacca ccgcgaatat
     7441 agccaaaagc tcctgcttcc atgcgctctt ttacacgacc ttctagttag gcaatgttta
     7501 caatatcgat tggatgttct tcattacttg tatgataagt catagtagat ccttctttca
     7561 tcttcttgat tacatataaa tcatactaaa cttacaaaaa gtaagcaaac atttactctc
     7621 agatactttt atcgctattc tttccaaata aatcgactta caagctgatc tacttcttgg
     7681 ttttcatgaa gcatactatg agaggcattt tcacctgtca ctactttttc ctctatttca
     7741 tttccgcgtt gcttcaacaa cgcatagacc gctaatgcac tgtttaacgg tactgttcca
     7801 tcactcaagt ctgtttcatc tagttgacca gcaatcaaga aaaatcgagt ggttatagga
     7861 atattaccga tcatttgctg gtaatcttga tagcggttag aaacgacagc aggccccttt
     7921 gctaactcat ctgtcagaga ctgcgcacta tcatcgacaa aatcattaaa aggcgcgcca
     7981 attgctgtta atgtctgaac ttgtggcaat gacgtatcct gtccatacgt tcctaaatag
     8041 cgtagtgcac taactcctcc catggaatgc cccactatgt tgactttatc tatgccgtag
     8101 ttttctttca gatacaaaag acaagttttg atccattctg cttgattcca ctcgttattt
     8161 ttattatctt caaacaatac ttggaccatc ggattgtctg cttttccaga caaatctcct
     8221 gttgcttgta tttgtccatc tgttcctact gttaaaacca attcttttac tgcttgctga
     8281 tttttttcca aacgccgcaa catcccttta aaggaatttt ctgtcccgct gtagccgtgg
     8341 aaaaaaagtg ttggaatagt ggttttttct atcactttac tggattctgc cgttttttct
     8401 ttatcagaaa cttcagtagt atttatcttc gtcgtctggc aaccacttaa cgcaaacaaa
     8461 ctgataagga ctaaagctgc tgcagttgca cgtttgcata aatttttgat tggttctgtc
     8521 atacacctct tcctttccat atagaaaaaa gaaagacttg aaaggccaat catatgatcg
     8581 acatttcaag tctctttatt aatcgatttt atctacttga tcgaaatcaa gttctgtact
     8641 ggtttcacgt ccaaacatat cgatgttgac tttcaatttt tctttttctc cgtcaacttc
     8701 tgttacttgt ccttctaatc cagcaaatgc gccttcgatg attttcacga cttcgccaac
     8761 ttctacttct agatcatttt gtcgcaggct cataccgata gaacgcaaga tatgattgac
     8821 ttcttctgga agtaatggtg ctggtttgct tccggctccg tgtgaaccaa cgaatcctgt
     8881 cacaccaggt gtgttgcgga caacatacca tgaagcgtct gtcatgatca tttcaacaag
     8941 tacgtatcct gggaatgttt tatgaacgat ttctttttct ttcccatttt tgacttcagt
     9001 ttctttttct tcaggaacaa cgacacggaa aataaagtcg gccattttca tactttgcgc
     9061 acgagattcg atatttgctt ttactttgtt ttcatatcct gaatatgtat gcaagacata
     9121 ccagtttttt tcaaaagatt ccattgaagg ctcctcattt cattgtaaaa taaaaaaacc
     9181 tcagtacacc cgaagttttt ctttcgcttc attatagcac taaaagtacg ttgtggctag
     9241 cgagttattt taaaatccag ccaaataagc tttggatggc tgtatccatt acgtaaaaca
     9301 tcaatccgaa aataatagaa gtttcgatga ctactaacgt atctttacgg agttgttttc
     9361 tgcttggcca agttacttgt ttgatttcat cttttacgct ttttagaaat ttcatgagac
     9421 aacctccctt gctactgttt atttcgtttc tttatgtaat gtatattgat tacaatattt
     9481 acaaaactta ttgatttcca aacgttcgcc gctttttcct tcactgactg attttgtgta
     9541 gttgcgagac ccacaaacgg aacaagcgag tgctgctttt ttctttgcca tttacttctg
     9601 cccccttgct gagaatcaga cttatacttc ataacagtat cacgtaactt tttacttgtc
     9661 aatttgtgaa ataaactatc tattgttttg gataaaatta tgatatgatt aatttgaaat
     9721 taagctaaaa tgagacaatt ggaggtatag cgatgctatt aaaaacactt gtatataaga
     9781 aaaaagatct gacaaccgtt acagagacta gtacattaga agaagcatta aaaattttag
     9841 aaacttccgg ataccgttgt gtgccgatct tagattcttc aggtaatatt ttccgtggaa
     9901 acatttacaa aatgcatatc tatcgtcata aagcaaatgg cggtgatatg acgcttcctg
     9961 tcactcatct tttgaaaaac gctacaaagt tcatttacct ggatacttct ttcttcaaag
    10021 tattctttac gatcaaagag cttccttata ttgctgtcct tgacagtgaa aatcattttt
    10081 atggtatttt gacacatagt acgttgctta atatgctctc tcaatcttgg agcgtagaac
    10141 aaggaagcta tgtactgaca attgcttcca caggaaagca aggagatttg gctgctattg
    10201 caaaaatcat ttcaaaacac agcagcattg ccagctgtat tactcttgat atcgaaaaag
    10261 acgaatacat tcgacgtaca ttgatcacgc taccggctaa cactgaagcg aaagtatgtg
    10321 aaacgatcgt caaccactta gagaaaaaga attataaagt ggtagaaatt gaaaatttgc
    10381 aaaaccaaga agcataatca aaaagcgaaa aaagaaaaca gctaatctgc tttctttttt
    10441 cgctttttat aattagacac gcttttcaaa atcaagcgcg cgatctaaat aatacatcaa
    10501 tggtgctgaa acagccatga cgataaactc acttaatgca agcgtgccat aggttgtcca
    10561 aaatgcttgg ccacctgttg gaactaacat ataagcaatc agtcccatgc tgatggtaaa
    10621 gaagatcgtg ttcaatacta aacgcatctt tacatttttc actttgtttt gcaataaagc
    10681 tgtcaggcct aaagaaagca atgtttggcc gccaccaaaa accacatcca tgataccaaa
    10741 accaaagaag gcattataga cgatgacccc gcctaaaact ccccaaagca atttacggtt
    10801 aaagacaact aaatgattca aactttctga aatacgaaac tggatcggcc ctgaggatac
    10861 aggcgcaact aaaatagtta acgctagata aagagccatg atgatgccat ttacaacgat
    10921 tacggataac cgttgtctgc ttttcgtttt ttgcattttc tctcctcttt tctcctagtt
    10981 tttttaacgc gggatggttg atgaaccgcg gttatgtgaa acaaacaata attataacag
    11041 acttttcatt tatttcaatc cgtcttatct gcttttaaaa aagcaaaagc ttaaacaatg
    11101 taaacgtttg ctaaaaatcg tgggatatgg tataaagtaa gtgtaagggg tcataagaat
    11161 tcccagtaaa gcagacgaag taggatctgc aagaacagaa ttgttgtgaa acttaaataa
    11221 ataaaaaagg atgtgggaat ttgagcaacc tcttactaga gaacggtcta gcaagcagct
    11281 gaagatctaa gtggacatgc cgaaccttgg aacttctcct tacgtaagta atggatcatg
    11341 cgcttgctag gcttttttgt gtttttcggt cttcggacct ttttcatttt cctagacttc
    11401 tgctataata accaagaatt aattttgtaa cagatttttt tgaaaaaaca agtttctttc
    11461 attcaaaaaa tagaaaaagc ttgctaatta ttggttaacc agcaaacagg agtattaaaa
    11521 tatcttgttt tattgaatag caggaggaaa tcatgaaatt ccattatcat atattgatca
    11581 atccatcggc aggcagcgga aatggctata aagtagcaga aagaatttta ccggtattaa
    11641 aaaataagca tattgactat acacttcatt atagtgaata caaaggacac gatgctgaga
    11701 ttgctgaaac tttggcaaaa gaaacattga ttccttggat agaagaagaa cagaaaacaa
    11761 tcgatacttt cccccttttg atcatcgtag gaggtgacgg caccttacat caggtacttg
    11821 atactttcta tcagatggaa gtagaattcc cagttgctta tattccagca ggttctggaa
    11881 atgattttgc tcgaggggca ggccttccaa aaaatcccaa aaaaggactt cagctgattt
    11941 tagcagctca atcaccagaa aaagtccaca taatggctta tgaagaaaaa ataagtgaaa
    12001 aaaaaggaat agctgttaat aatttcggta taggattaga tgcagctatc gtccatgcga
    12061 caaatcattc ttccacaaaa aagcgattga acaaatacaa cttaggatca ttttcgtatt
    12121 tattctctat tctccgtgct ttattcactc aaaaaggatt tccaattctg gtagatgcag
    12181 gaggaaaaca attaagtttt tcaaacgcat ttttatgtac agcaacgaag catccttttt
    12241 ttggcggagg aatcgcgatt gttccaactg ctgatgcgtc taaaccagtc attgattttg
    12301 tcgtggtcga acgaatcaat ttctttaaaa tcttgtggct cattctttta cttatacaga
    12361 aaaaacaatt gaaatcgaaa tatttccatc actacacaac aaacagatta cgtatcgttt
    12421 caacaacacc gcaacatggt caagaagatg gagaagaaat ggaaaaacga ccttttgata
    12481 tcacaataac cacaactaaa cagttgtttt ggtgttaata aaagaggttt tgtcagaagc
    12541 tacatctgga acaccgttta ttcggaaaaa aggactgtga cacgactttt gtcacagtcc
    12601 tttttttact atgcagttaa ttctttgccc aattgggttg ccatcgatag tttctttctt
    12661 tacccaaaat aaattggatc attgaaaaca ccgcagcaaa agcaactaag acacaaccat
    12721 tgaaccaaaa agattctgca aatgtttgat tgtaactgag tgtttcactg atcaagttat
    12781 tattttgaac aaaccaatat gttggtgtga atgccgctat tttgctaacc atttctggca
    12841 agtattctgc tggtacaaac accccaccga taaagcaact gcctgtgata aaaatatctt
    12901 tgatcccacc gctggtttct gagtttttta ccaatgaagt gatacaagcc gaaaagctaa
    12961 tgatcggcaa taaaaacaag aaagtattta aaatatgata ccataccgtt atatcccacg
    13021 aggtgtggat cactaacaga accattagaa gtaagaagcc actgaacaac agcagcgagt
    13081 aactaatcaa actaaaggtt actttgcgat tcaagtttcg tttggtaatg ggcgcactct
    13141 ggtttcgttt actgatctct ttgcgattaa acgctagatt gatgacgctg aaaccgctaa
    13201 aaatggatag aaacaaccca taagacaaga aattaaaggt tgtgcccgtc aacagctttt
    13261 tcatccgctg actatatccc gcacttagct caacttctcc agtaaccgag agtgccattt
    13321 gtgtctgttc catagctgca tcgattgttt tatcttcttc ttgaaacaga atcagtgttt
    13381 gcaaaaaggt gttcacttgc tgtgtaacaa ctgttttaga aaaggcatct gggcgactct
    13441 gtatcttgat cgtcgccatc tttcctactt cgacttgttc ggtaaagtct gcaggcagat
    13501 gtaagataat gtttacacga ttaaaataaa gagcatcatc gatttctttt tgtgaggttc
    13561 catccaatgt aacgactgtt tccttgtcat tcaaatattt acgaaggcca tcgactacag
    13621 gcgactcttc ttcactaatc aaggcgattt tcgcatcttg cagagaggta ttttcaacat
    13681 caatattttg cgcaaaaaag aatgtggcaa caacagtgat aattacacct atcagtaaat
    13741 tcgatttatt tgctttaagc aatgtccaaa tcgttttata aagcgtcata acgcacccct
    13801 ctttccatcc aagcatttcc taacgcaaaa aggaaaataa atccgatcaa ccatagaaga
    13861 ttggtataaa aactagccaa atcagtgtag aaataaagtt tatacaaact ttcgctgatc
    13921 aagttgacga gatttaattg accaacaatc ggaatatgaa gatcaatcca atacttcact
    13981 tgttccgacc ccatcatacc agcaaaaaag ctcatcgcaa tcgtcactga aatcaagatg
    14041 ctatcctttt gcaccagatt catttttggt atcaaattac caaccaatgt acctaacaaa
    14101 atcgcacaca acgaccccag agcggtaaca atcatcaacc attgccaacg ttcaccaaag
    14161 tctacttgat agataaaacg aaagacagct aacataatat aaatctctgt caaaacaatc
    14221 gtaaagcttg ctgccaaatt agcgattgaa actaacgatt gctttctagg aatcaaggac
    14281 aagcgaatac catttgacga ttgatttgct tgctgatcct gtacattgcg gaccccccac
    14341 atcgtcccgt agagaatcgc cattccaatc agagtgaaga aataaaaact tttgaaactc
    14401 atattacgac ctgcatgaac ttcttgaaaa gatacttctt gttcaaaaac agtcatgatg
    14461 ggcgcgagat caacgttctc cacttctgcc actttactcg aaattatacc aatacgctgt
    14521 aaaaattgat ccatgacgtt tttcatcaac gtctgactca ttcctgcttg acccacatgg
    14581 agcgaaacag atgaaccatc aatgagataa aaaccagcca aatcatctgc atttagcttt
    14641 tcattcgctt ctacttccga tagttcctgt acttcaaata atggtttatc attgacttcg
    14701 acttgttcta agactgtggt aaaattttcc gaaagcgctg tttgatccgc tttgattaca
    14761 attccagtat taatcgtttc aagattgcca ccttgatcaa tctcgccaaa ggccgccata
    14821 aacatcaatc ctaaaagaat tggaaaggcc aatgtccaaa acaacatgga tttatttcgc
    14881 agcagtattt ttgtgcgata tttataaaaa tgccaaaaca ttatttcccc tcctctagtc
    14941 tctcaaatcc ttgccagtaa tctctaaaaa gacatcattc aaggacgctt ctttggttgc
    15001 tatttttccg taactgatct tttcttcttg taagattgta agaatcggca acaatactgt
    15061 atttagttca gtagttgtta cttggagctg acctttttta taagcgattc catcagaagc
    15121 tattgttcgc tgaatcgctg acaacacatt ttctgggagt aatgggatgt cgatcgtcaa
    15181 acgatccgcc gtccgtacca tatcttttag agattctttg gttcctttag caatcacctg
    15241 tccttgatcc atgatcacga tctggtcgca aagaatttct acttcttcca tgtaatgtgt
    15301 tgtatagaca atggttgctc cttgtcgatt cagctccttg atactttcta aaattttatt
    15361 ccgactttgg gggtctacag ctactgtcgg ctcatctaaa aaaattaacc gcggcttgtg
    15421 ggcaatccca catgcgatgt tcaaccgacg tttcaagcca ccactaagtt ctcctggacg
    15481 aaacgaaaca tatttttcta atcccactaa ttgaatcacc tcttcaatca aggctttcct
    15541 tttctctttt tcggaaacat ataaaccgca aaaataatcg atattttctt gcacacttag
    15601 ttcgtcaaag accgcaacgt cttgaggaac gaccccaatc attgccttga cagcataatt
    15661 ttcaggagtc attgttgtcc cgaacaattt aatggttcct ttgtcataat ttaacaaaga
    15721 aagcatacag ttgatggcag tcgatttccc actgccgttt ggacctaaca gacctaagac
    15781 ttctccttct tgtacttgca gatcaaaatg attcaatgct acaaaatctc catagcgttt
    15841 gactaattta tcaatttcga taatcatcat gaaaaactcc ttccaaattt cttgttgatc
    15901 tgactataat aaagtgcaag aaaagaaact agtgacgatt ggctaaacca cacatgacat
    15961 ttgtcacaat gaaggagtct gctatgcata aatctcggct actcaacgaa actagctttc
    16021 tactggttat ctcattgctt ttgctggtaa gtcaaggatt cgttgctatg caagttatct
    16081 atttattgat cgccgtttta ttcactggtg tcagttattt actgcctaca aagccaaatt
    16141 ggattttcca agttattcta tttcttttat gtgccttttt acctgatctt gtcctgtttc
    16201 tgccagcgac gatgcgagtg atcgcccctg aagaaagcca ccttgattac ttgtttttgt
    16261 taggtttttt gttgatttcg acggttggta atcatccccc catcaaggtc gcctttgtcg
    16321 taattttgct gtttttttcc ttttacttac gaaaaaaaga tcaacgaacg accgatcttg
    16381 agaaaaagta cattgtctcc aaagatactc attgggaaaa acaacaagag ttatcggaac
    16441 aaaataaagc attggccctg tcacaagaaa cacaattagc cttgcaaatt gtggaagaac
    16501 ggaaccgaat tgcccgagac atccatgaca atgtcggtca tctgctgtca agtgcgattt
    16561 tacaaattgg tgcgcttgaa atgatcaatc aagaaccaaa actaaaagaa ccattagaaa
    16621 atttaagtgc caccgttcac actgggatgg atcggattcg tcaaagtgtc catgacctcc
    16681 atgagacatc gatctctttt caacactcct tagagtttct gatcgctgat tttccttctt
    16741 ctgttacgat tgaaggaaac ttgtttgaag gattgaatga aacgcagcaa aactgcttct
    16801 taatggtcat caaagaagcc ttaaccaaca gtatcaaaca tagtcatgcc aaaaccgttg
    16861 ttttacattt tcgaacgttg ccggcctttt atcgcttgca aatcacgaat gatggcacca
    16921 gcccacaaaa aaaatctgct cccggtattg gtttgaaaac gatgcgtcaa cgtgttcaag
    16981 aacaaaaggg acaagtccat attttccaag aaaaccagca atttcagtta acgatcattt
    17041 taccaaggga gggacaccaa tgataacagt agtcattatt gatgatgatc catttgtgac
    17101 gaaatcgtta caaaccattt tagaaagcac gaaagagatc cgtgtgttag gcattggtca
    17161 ttgtgccaag gatgcgctag tcttatacga aacccatcgt cccgatgttt tgctgacgga
    17221 catccgcatg cctaaacaaa cgggcatcga tgctgccaaa gaaattttgg ctaaatttcc
    17281 caaagccatc cttttgcttt tgacgacatt caaagatgaa gaatacatcc gcgaagcatt
    17341 ttccatcggt gtcaaagggt atttaatcaa acagaacttg caagcgatta ttccttctgt
    17401 caaatccgct tataatggac aggccgtatt tggtaatgaa atcatcgaga ctttcactca
    17461 aatgatgaag aataaaccga cgattgatag tcatgcttat gcttctttta gtgaacgtga
    17521 gcttgcaatc atcaaagaag tagctgccgg gaaaaacaat aaagaaattg cagatgcctt
    17581 gtacttgagt gacggcactg ttcgcaatta catcagccaa ttactagaaa agctagattt
    17641 acgggatcgc acgcaattgg ctatctattt ttatcagcat ttgcaataag tcttatctaa
    17701 cacaaaaaaa cctgaatgat gctccttcgc atcattcagg tttttttcac atcttctatt
    17761 caattgtccc gcaaaccatt tcctcacgat tgaaagaatc aaggattcgc agggctgcca
    17821 ggattcgttg gattgttcgg cgtgttaggc ggaaaaatag gcgccttgtc taacgttttt
    17881 tctagttttg attcttcacg atcgtctcgt tctacatcag gactttcatt caatttgaat
    17941 tctttttcca tctcatctaa ctttttaaag tcattgccag attcttttgc taatttcgct
    18001 aatttatctt cggtttcttt agccataaag taacgccccc cttttgattt cagcttagca
    18061 tatcacactg aaaaaacaaa agaaaacgct aaccaacgat catttttagc tatttctcat
    18121 ctttaaaatc aagcattttt tgccggtata aaatcgtagg gagaactgtt ttccatacca
    18181 atcatcggat ctactaactg gttatcgatc acttctgctg taagaacgaa atctttagaa
    18241 acagcaggtt ctacctcttc tttcttttct tctccaacaa atgaagaaaa cagatctggt
    18301 tccactgttt ccattacttg gtttttcgtt tcattcgtcc cactgtcatc ttctacaggt
    18361 agcttttctt ttacaacccg tggctctttt gtatagttcc catcttcaaa tggatcattt
    18421 ccgaccaaag aatcttcata agctgctagt ttggtccgga gagtgatatt cattttatca
    18481 ttatccatga tccttgattt caacgtagtc atccggcttg ctgattcatt cgctacacaa
    18541 gtgacatagg cttgctcttc ccctaacaaa atcaacagtt ccttactgcg agtcactgcc
    18601 gtgtagagca aattacgctg aagcatccgc tgatattgat ggaccatggg caaaataacc
    18661 atcttgaatt cactgccttg tgatttatgg attgagcaac aataagaaag agtgatttta
    18721 ttccattcgt ttcttttata agtgacctca tttgcatcaa actggataac taattcatcc
    18781 actttatctt cagaatcttt tgccaagatg atacccgtga tttctcccat atctccattg
    18841 aatacattca attcgggagt attgactaat tggaggactt tgtctcctat ccgataaacg
    18901 gtgtcgttcc aatgtacttc cttttttcca ggtttccctg gattgaatat ctcctgcatc
    18961 attttattta acgcatcgat ccctgcaggt ccccgataca ttggtgctaa tacttggata
    19021 tcttgtgcac ggaatccctt atttttcgct ttttccacaa ttttagagat caagggttca
    19081 atttgataag catcacttcg gaaataggaa cgatcttttt gattctttgt gaaatctttt
    19141 ggcagacgtc cttctttgat ctcatgggca agaggaataa tactagaacc atcaccttgc
    19201 cgataaatct gattgagttc cattttggga atttgttcga tttccaaaag atcatggagt
    19261 acttgaccag gaccgacaga gggaagctga tctttgtctc ctactaagat aacttgcata
    19321 ttcgtcggaa tcgctttgaa taatgtgtta gccagccatg tatcgaccat agacatctca
    19381 tcaacaatca aaagtccgcc ttctaattct ttcgtactga ctgaaggtgt tttttcccgt
    19441 ccattcagcc ccagaagacg atggatcgta ctggcaggca gacctgtcgt ttcgttcatt
    19501 cgtttagctg ctcggccagt tggtgctgca agtagaatag gaaaaatttc ttgggtatat
    19561 tttgaaggat ccaattccag tccgttcaac tctgcaaaaa gattgacgat tccgttgatc
    19621 acagtagttt tccccgtacc tggtcctcct gttaaaataa atagagggga acggattgct
    19681 tcagcgatag cttgttcttg cgaaggtcca tactgtatgc cgaatatttt ttcgatacgc
    19741 cgaatattct tctgcacttc tttttcagga taatggattt cttttttacg actgagaagg
    19801 cgctggatcg aattaccgat cccccattca gaaaagtaca aactgttttc gtaaagatta
    19861 gtctcttctt gttgtatctt tccttcttca acaagttgga taattacagt agcaacctga
    19921 tcaggctgga tttcaattgg gcgacttgtt tctaaaatac ggatcgtttg ctgaagcaat
    19981 gtctccgccg gtacgtatgt atctcctgtt tgaactgcat gctgaaaaat ctgatgcaag
    20041 atagctgctc gaattcttct cggtgaatct gctgcaatac cgatttgttc ggctagatta
    20101 tctgcacgtt tgaacccgat tccttctatg tcttcgacta gctggtacgg attttcttcg
    20161 atgatatcca aagactcgtt cttataggcc tggtagatac tgaaagctaa ctgactaccg
    20221 aacccgtaac gattcagtcc catgatcact tgatccatcc cgtggtttag ccgaatcgtc
    20281 tcagcaagca tttctctttt tggcttcgtc aaaccggaga tttgttttaa taccgaagga
    20341 tcagcaatga ttttttcgat agcatcttct cccagcaatt ctacaatttt ttctgctgtt
    20401 ttcttcccga ttcctgggaa tttttcgctg gaaagatacg ctacgatccc atttgcagtt
    20461 gttggctttt cttgctcata tctttcgagt ttaagctgcc ttccgtattt aggatgatcc
    20521 actaattctc cataaaaacg ataagtctca ttttcttgaa tatcgccgaa acttccggtg
    20581 actacaatct ctttttcacg gtaatcagct gtggtttcac tgatgtgtac caaaagcact
    20641 ttgtaaaaat tactaggatt ttgaaaaaaa atcgcggcaa ctgtaccaac gaaataaggc
    20701 atttcttctg gctgcatcgg aagccgcctc cttcttctat agtaaaggtt ctggtgctgt
    20761 ttcagttcct aaaaaatctg tttggttcca tactttcagt tcatatggca ataatctgca
    20821 aggctcttca gtctctaaaa tcgtcaaact actattgaac aatccaccca tttcacgcaa
    20881 ctcacttagc tcttttcctg tcatccattg aatggctgca gttaaagaag ctccatgtcc
    20941 tacgaataaa agtgggccat ctttatgtcg ggcaaccgca tctgctacaa cagtttcgat
    21001 acgtgtgatt gccgattcaa tcgtttctcc gtgaaaagga gaaggatcat acagatctaa
    21061 atgattacgc aaattcggca gattatccgg atatttctgg tacatttcat cgatcgactg
    21121 accttctaaa gtacccagcc ctagttctct tagcttatct gtataaaaaa tctctaccgg
    21181 acgagcaagc tctttgttga taccttctgc tgtatcacgt gctcgctttg ctgtactgga
    21241 ataaattgct ttaaaaggaa catattttac ctttttccca aatgctttga tttcttcgta
    21301 actttgtggc aaaagcggag aatcgccatt gctcccttga aaacgtcttt cctgattcca
    21361 ttgtgtcttc ccatgtcggg taaaatatag ttccatacgc agcaaactcc tgtctttctt
    21421 ttattttact ctataataaa ctgtctaatc cagcttgtca ttttcatagt gatgcgtcaa
    21481 ttctaaatta gggaatcctt gctggcgaag tgcttcatac actatcattg cagctgtatt
    21541 cgataaattc aatgagcgga catgctcatc attcataggg atgcgtaaac acttttcttc
    21601 gttttcacgc ataaatgttt ctggcaaacc ggttgtttct tttccaaata aaaagaagtg
    21661 ctcttctcca tcagcaaaat tttcgtcact atatacttta tgggcaaatt ttgagatcaa
    21721 atgtagttta cgattaccta aaaatgttaa aaaagcttct aaattactgt ggtaagtaat
    21781 atttacatca ttccaataat ccaaccctgc ccgttttaga tgtttgtcat cagttgaaaa
    21841 acctagtggc tcgattaaat gaagcggagt attcgtggct gcacacgtac gggcaatatt
    21901 tccagtgttt gcagggatta atggttcaaa taaaacgata tgattcatca ggatcattcc
    21961 tctcatattt atgttcgttt ctattttctg acaatttgat ttttgcataa aaaaaaacgt
    22021 atcaactcgg tgtcagtcac tgcgagtcgt tacgttagtg aagctcttct cactaacttt
    22081 tatcagaaca ggaacctgat aaaaaccaat gaaaaaacaa cttgttgcta atataacacc
    22141 ctcaactttt ttttgcaacc tctactcatt aaaaactgct agaaaaacgt tttctcttag
    22201 ttccttttaa taattctatg tcaattgtaa tcttcttcaa aggattctta tataagtctg
    22261 ctttgattcg ctcttctacc tgccattcta aaggtgacat ggccaaaaga tctttttgat
    22321 tttcctctgg tatctcaaat tcataggtta cacgctgacg ttcagttaat ttcattgctt
    22381 cagcaaactt ggctactaca cgttcattgg aataactctg tttttcaggt tgattcggat
    22441 aaaaagcctg acgtagttcc tttaaataat tttcttgtgg caccaccttc aacacttctc
    22501 catcttctga gagtacacgt tggaattctt ggtaatgaga aggcgaaaag atatttaaaa
    22561 ccgcgttgaa tgtttgatca gcaaacggca aattggtcaa atcagctaca caccaaaatg
    22621 cttcggcaga ttgttctgta gctgcgtaga ctccttcttt tgaaatatca aatccaacag
    22681 cattttgaat ctttttcaat tttgaaagtt cattcaagaa gctaccttct ccgcagccaa
    22741 catctaatag cgagacttct tcaggtaaat gctgcgcaat agtctcaata acgggctgat
    22801 acatgccaga ttggatcatt ctacgccttg gaacgaacat tccttgatcg tattctgttt
    22861 taaccgcatg ggataaaaaa taaagtgttc cttttttgga aaggtcaaaa cggtgattat
    22921 ttttgcatat caagccatta tcagactttt ttacttcttc ttggcaaacc ggacagcgga
    22981 acagctgatg attttctgcc aaaaactggc gtcctcggtc aatttttttt aacatagttc
    23041 cccctcattt ctgttttatc ttaccgttag atgctcactt tggcaactgc taaaaatatg
    23101 atacaatgcc tagaagaaag tgaaagataa aggagaagat tagatgatca aagaattttg
    23161 cgctgaaaac tacacatcta ttcctctagc aattgctaga ggagccgatc ggatcgaatt
    23221 gtgtgacaac ttagcggtcg gtggcacaac cccgagtact ggtgtgatcg aagaaacgtt
    23281 aagttatgct ggggaaaaag atatccccgt aatgacgatt attcgtccta gagggggcga
    23341 cttcgtctat aacgatatcg aattgaaaat catgcatact gatttgattg aagcaaagaa
    23401 attagggacg gatggcgtag tatttggttg tcttactcct tcaggctggc tagacgaaga
    23461 ggccttagaa ctattgatcg aaacagcaga agggttgcag atcaccttcc atatggcttt
    23521 tgacagtatt cctgaagatc ggcaattcga agcaatcgat tggctggtag aacatggtgt
    23581 tgaccgtatc ttgactcatg gcggtcctgc tgacaaaact attgtagaaa atttcccacg
    23641 gcttaaagaa ttgattgcct atacagatgg ccgcttaatc attttgcctg gtggtggtgt
    23701 cactgcagag aatgcagcac atgtagctaa agaattgaac gtaagcgaag tacacggaac
    23761 caaaatcgtg gatcttcagt tataatctta taaacaaaaa aacatttgca acaggcataa
    23821 tgcttattgc aaatgttttt gtttttttac ataaaatagc ccataaataa agaagcaata
    23881 ttgaaataaa tcagtacact cgtcaaatcg ctcaaagtcg tgataaaggg accgcttgct
    23941 actgctggat caaatcctag acgatccatt agcatcggga ttaaactacc tgcaagattc
    24001 gccactgtga ttgcaaatag catagccatc cctatgacaa accccaagat aaaattatgt
    24061 ttccaaaaac ctacgattag aaaaatcgtg atccccgtaa cagctcctgt gacaacgccg
    24121 gtcaagactt cagccaaaac aagtcgaaca aatccatgat ctttctcatc agaaatcgca
    24181 agtcgacgaa cagccacagc tagcgactgg gttccagcat taccggcagt tcctgtaatc
    24241 agcgaaatga agactgctaa aatactcgct tcacttacca agtcttcata gtgactgatt
    24301 aaagaagcgg tcgacatacc taaaaacaac agagtgatca gccaaggcaa acgtcgagac
    24361 gctgccttta ctggattttc attaatttct tccacattga ccccggcaag tcctgaataa
    24421 tcgctagcag cttcatcatc aataacgtcg atgatatcat cgactgtgac gattcctaac
    24481 aaatgatcat cataatcggt aacaggaaga gctaagaagt cataatctct aaatgtttgt
    24541 gccacatctt cttggtcgtc gcctacatga actgaaagca ctcgttcgct catcagatca
    24601 gaaatcattg cgtcgtcatc attcacgatt aggtcgcgca gagaaataac ccctactagc
    24661 tgaccttctt gattaacgac atagatgtaa tagattgttt cagctacatc tgcttcgttc
    24721 ttcagcacgt acatcgcaga gcgaacagtt tgattggcta cgatcgaaac aaattcagtt
    24781 gtcatgatcg cgccagccgt ttcatcctcg taatggagaa gttccttgat ttcactggca
    24841 tcgtctgccg acatcaaact taaatattta gcaatttgct ttttatccag cgtattaagc
    24901 aagtcaacag cattatctgt atacatctca gaaagcatat cagctgcata gctaggacgc
    24961 atttcatcta ggtattcttt catatgttcg ttgtcttctt caatcacatc gaacatatcc
    25021 gctaattctt taggcgacag ataggaatag atcagctgcc tgttctcttc tgtaagagat
    25081 tggtagaatt gcccctgctc atagatatgc atctctaaaa aactgtttct gaatgcctga
    25141 atattatttt cccttaaatc ttgagtcaac tgggcaaatc tttcctctaa ttcttgattt
    25201 tcatccaaac caatctcacc tcgccttatt cttcaaaccg tctcacaact gcctccatgt
    25261 ctgcggctaa aggctgcttg aactccagca actcttctgt aaatggatgg taaaaacgca
    25321 gatcataaca atgaagggct tgtcttttta ttcccagatc cattcgcccg ccatacatat
    25381 catcaccgag cagggcacag ccaattgcgg aaaaatgaac tcttatttga tgtgtccttc
    25441 ctgtatgcag ctggatatca actaacgcga tttctgttag tcgcttctct atccagtact
    25501 cagtctgtgc cgaacgtcct tcatcagaga ccattcgctt caatagcgaa gaataatctc
    25561 gggcgatcgg caaatcgata tgtccatgtg ccgataaatc tgaaagcctt ccactgacta
    25621 aggcctgata ttttttcaca aacttctttt cacgaagttg catatctaat tttgcgtgcg
    25681 caaatccatg ctttgcaaaa agcatcaagc cagatgtatc ccgatctagt cgcgtcacca
    25741 catggattac ttggttttcg taaccttgtt ttttataata agcctttaca cgattagcca
    25801 ttgtcccatt tggatggtat tgggcaggaa tagacgaaac gcctgctggt ttatttacta
    25861 ccagaacgtg ttcatcttca tacacgatat cgatcggtgt ttcatccaaa agtacggttt
    25921 catgttctcc ttcagcagga atcacaatgg ttaccttatc atccttagct agagaaaaca
    25981 agacattttc tacttgatca ttaactttga tttgaccgcc ttgaaatttt attttcgcta
    26041 acagaccctt tgagatccct ttttctttta aaaaatattt tacttgctga gaatgatctt
    26101 tctcataatc ccatgtaaac tgcatatcaa tcctcactaa taaaggcatc tttcacacga
    26161 tgccaaaaat gcatatgtcg ataagaagca aaatggatcc gttcttctgc tattcgataa
    26221 taaattgagc ggatatttgc ttttgcgaca ttcaattgat caaccgttac aagataatct
    26281 tctgtatttt ccaattttat ttctagccat tcatcatggg caatcacgat cggagaaccc
    26341 aaagtacgaa acactcgatt attcagtgaa gcaatctctg ccaactgaaa ggcattgata
    26401 ctaggatgga tcacagcacc gccgacactt ttattatatg ctgtcgaacc agtaggcgta
    26461 gaaatcgtca atccatctcc tctaaaactt tcaaacagct cgtttttgat gtatacatcg
    26521 gctaccatcg tacgattggc ccgtttaatc gttgattcat tcagtgctaa aaaatgctgg
    26581 tccggtgttt catccaaata actgatccga acatccagca agggataact aacactttgt
    26641 tcccggttcg ttcgtaagct gtctactagt tcttccagct cgtaatcccg ccaatccgtg
    26701 taaaacccta aatgacctgt atgcacgccc aagaaacgca catcattcag acaatgactg
    26761 tagtgatgga acgccgacaa taatgtgccg tctccgccga ctgagatgac caattctggc
    26821 tcatgatcat ctcgttgaat ccctgcttgt tctaaaagtg cgttcaaacg ttctgtcact
    26881 tctatagtct ttgcttcttt attatgaacg atcgctactt tcatcccctg cctcctctct
    26941 ttcgctttct ttcttcaaaa gcgttcgata gtagacatca tctgattttc ctcttccatg
    27001 ggagaataac cgctgtgctt cttggatttc ttcacggata ctggacatct cttcatccaa
    27061 ctgataggca gcttctgccg cacgctgaag tcgttcactc atttcttctg gaaaaacgcc
    27121 ttgatattta taattcagcg aatgctcaat cgttgcccag aaattcatcg caagagtccg
    27181 aatctggatt tccgccaaaa ttttcttctc accgctgatc agctgtacag gatactcgat
    27241 caccagatga taagaacggt agccacttgc ttttttattt gtgatgtaat ctcgttcttg
    27301 gagaactttc atgtcttttc ttttacggat gatctcaaca acttgatgga tatcttctac
    27361 aaactgacac atgatcctta acccggcaat atcagacatt tcttcttcta aacgtcccat
    27421 tggaatatgc cgtaattgtg attttgttac aatactatcg actggcttga ctctcccagt
    27481 gacaaattca atcgggacat gtcgattttg ttcacggaac tgtttgcgga tcccccgcag
    27541 tttcactttc aattctgata cagtttgttc atatggtgct aaaaatgctt cccaatctcg
    27601 ttccatagct gatacctcta cttcattaac attcattgtt tattctacca tatatgcaaa
    27661 gttttttcgt actcttgatt tatgcattga ccaaaaaaag aaaattaggc aaaatagaca
    27721 aaaaggagtc gatcaaatga cacaaatgct tgaaatcgaa tataaatcta tgctgacaaa
    27781 gcaagagtac gagatattca tcgctcacta tcaattaaca gataaagatt tccgtgtaca
    27841 gactaatatt tatttcgata ccgttgatga acagctgaag aaacaaaatt gtggcctacg
    27901 gatccgtttc gttggtcatc aagctgaata tacattaaaa acacctgctg aaaaagggcg
    27961 tctcgaaaca acggatacct ttataacaga agaagcagaa gcatttatct tcgaaaaacg
    28021 tctgcctcgt tcaggcgcag tttttcaaaa actttctgat ttgaatatcg atcctgcttc
    28081 attaatccaa accggaaagc ttacaacaaa acgagctgaa ttccctattg aagaaggact
    28141 tttagctatt gatgaaagct ggggagacaa tctacacgat tttgaactgg aactagaggt
    28201 aggggatgca tctgtgggaa aaattgcttt tataaatttt ttaaaacgtt tttctttacc
    28261 ttatcgtcct gctaagaaca agatccaacg tatgcttgaa gctaaaaatt agaaaccttt
    28321 cattttcaca aggcacttga gttcattttt ttactttttt ctgttaagtt ataggatgtg
    28381 aaagaatcta gtcactagtt gtatagaatc tattttgggg gagattcata tgatcgaaat
    28441 ctatttattt gttaatcctt taggggaata ctgtttcgaa atggaacaac agttattaca
    28501 atttatcgaa gaagagtacg ggacaccatc aaaagaaaag atgcaatttc gctttcttcc
    28561 tttggtcaat ttgcagacaa ttggggatgt catgcaacaa aaaggaattt ctcagaacaa
    28621 tcttgaggaa cgtaatcact tattctcaat gacttattct gcagcattgg actgcaaagc
    28681 ggcgcagttt caaggaaaga aaaaaggccg ccgttttttg atcaaattgc aagaagcagt
    28741 cggctgtaat gacataccat attctcaaga acttgctgaa tccatatttg aagaaatcgg
    28801 cggcgatttg aatatgttta aagaagatcg tcagtctgac ttcgttaaag aagtattctt
    28861 ttctgatcaa aaaattgctc gggaaatggg catcacaaaa cacccttctg cagtcgttta
    28921 caattatgcc tgcgaccgtg attttggtgt tttagtagaa gtaaaaggta caagtagcct
    28981 ttggagaatc acagagctgt gtaaaacaac agataactca tatcaagttt ttaatggtga
    29041 acatttacat acaaatgatt ctcagttcca ttcaagaact agccatttac acttgctttc
    29101 taattaaaa
//
LOCUS       DACGLE010000030        28529 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0534
            SAMEA3304449-rid9560453.denovo.031, whole genome shotgun sequence.
ACCESSION   DACGLE010000030 DACGLE010000000
VERSION     DACGLE010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304449
            Sequence Read Archive: ERR985064
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 28529)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28529)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702225.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702225.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 20:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,045
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 27 of 67
            Pseudo Genes (incomplete)         :: 25 of 67
            Pseudo Genes (internal stop)      :: 19 of 67
            Pseudo Genes (multiple problems)  :: 4 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28529
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304449-rid9560453.denovo.031"
                     /strain="VREN0534"
                     /db_xref="taxon:1352"
     gene            complement(45..1241)
                     /locus_tag="IXT20_001434"
     CDS             complement(45..1241)
                     /locus_tag="IXT20_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HAQ4859888.1"
                     /translation="MDHKPTETKDVTPVNDTLEDLLNNPFSTPTDTLTTSQQAEIDQL
                     QEQQTAARLIDKLPAERQEQAKQLAAKIDANDAQSVISYGSAAQAKLSEFSQSMLNHV
                     QAQDIGPVGDSLTELMYRLQEANPDELRAGEGNIFQRVFGKVKQSIYEVTAKYQKIGA
                     QIDKISVKLDKEKDGLLKDNLMLEQLYQKNKDYFDALNIYIAAGELKMEELQTTIIPE
                     AMKRAEETGDQMDVQIANDYTQFLDRLDKRTHDLRLARQITIQQAPQIRLIQNTNQAL
                     AEKIQASIATAIPLWKNQVVIALTLLRQKDAVTAQRQVSETTNDLLKKNSEMLKISAI
                     ETAKENERGIVDIETLQTTQNDLIETIQETLRIQKEGKEKRRHAEVELGHMEEDLKQK
                     LLDLTQ"
     gene            complement(1365..2027)
                     /locus_tag="IXT20_001435"
     CDS             complement(1365..2027)
                     /locus_tag="IXT20_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-bromo-4-chloroindolyl phosphate hydrolysis
                     protein"
                     /protein_id="HAQ4859889.1"
                     /translation="MIKRNWPWLLALVLLFLFVRNNSFSFVLGLLLIGVVLVLWGLFG
                     SRRKKGKQEEIPALTDELETHYEKSGMTANEISFFRQTMNQTKNEINQLQMNMQQTAK
                     LKAIDLRHDPVKAAKALFKELVKEPNRLHEASQFLYTHLPNLVDLTNKYNEINDHEIK
                     NKQTYEKLEESAQIIDQLSALIAQDYQKFVAEDLDDIDVEISVAKQSLKRDNKYEPEQ
                     KD"
     gene            2255..2818
                     /locus_tag="IXT20_001436"
     CDS             2255..2818
                     /locus_tag="IXT20_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HAQ4859890.1"
                     /translation="MLNNEQFEEKTISRKEIYSGKIIDVAVDEVRLPDGGTSKRELVF
                     HPGGVGIIAFDEQDRLLLVKQFRKPLEKVILEIPAGKIDPGEGQNPEMTAARELEEET
                     GYRAKSLSHLTSMYLSPGFANEVLHIYHAQGVEKVENPLAQDEDEVLELYHLTLEEAQ
                     QAMKDQLICDAKTIYAIQYWELLTKGK"
     gene            2831..3115
                     /locus_tag="IXT20_001437"
     CDS             2831..3115
                     /locus_tag="IXT20_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4859891.1"
                     /translation="MAKGPLITRSELRKRQQAQASESLKKQRKAETAYQQEEKKIASF
                     YRKESKKNKPITKTRISEREKTTKWNSFLMKSLIIVILMLCVVFLAIAFI"
     gene            3134..3826
                     /locus_tag="IXT20_001438"
     CDS             3134..3826
                     /locus_tag="IXT20_001438"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HAQ4859892.1"
                     /translation="MKIGIIGAMEEEVKILRENLSEPLSWERAGALFISGSLGNHEVI
                     VVRSGIGKVLASITTSLLIQQYGVNMVINTGSAGGIGEGLQVGDIVISDKVAYFDADA
                     TGFGYNPGQLPGMPLYYEASTYLRSEMVKAAKATNLNAKEGLIVTGDTFVDSPDKIKE
                     ILTNFPEALACEMEGAAVGQTARQFNIPFLIVRAMSDTADHSATQSFDEFIEDAGKRS
                     AEMVIEFVKHLV"
     gene            3838..4383
                     /locus_tag="IXT20_001439"
     CDS             3838..4383
                     /locus_tag="IXT20_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAQ4859893.1"
                     /translation="MNALISIDYTYDFVAEDGKLTTGTAGQVIEKKLTAHTKKFIDQK
                     DFVVFAIDAHDPKDSFHPENRLFPPHNVIGTGGRELYGSLKSLYQDYEQEENVYWIDK
                     RHYSAFSGTDLDIRLRERQITDIYLTGVCTDICVLHTAVDAYNLGYKLHIFKDSVASF
                     DPVGHEWALRHFESTLGAEIL"
     gene            4654..7932
                     /locus_tag="IXT20_001440"
     CDS             4654..7932
                     /locus_tag="IXT20_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1565 domain-containing protein"
                     /protein_id="HAQ4859894.1"
                     /translation="MLKKRIKYSALMMNLLMLSTPVLISVVEVAANEQQSVNEENAHV
                     NIEGMSTMEEIPRTSEDMDTEEGNDPMMKESENEKTKKQSEEANEEWKGVVFGESTSA
                     SRYAIEPLEDGVRLSSTNNGGKFQSSGSDGMTYYYQAIPKDINFRMRATIEIESWTYT
                     NAQEGFALMVRDAVPKNHLFGQAFYSNSFAILGSRIEYVWDSDRQEVVNTSGSKYTMR
                     LGLGTRAITGISSEDPQAAPAPGSVSVETTPLETSAGFLEKETGSYNIFGNGHGNLFP
                     ATNSITKLDVEMKKTNTGLEAYYYDPETGEEMASDIMYDWEKLYASDESVIYAGFAAA
                     RNMTIKVEGIEVAYSDPATDPPGQERPTRLIDPIYTVTSSNHSGNQDHTMSFRANADG
                     LLTIKNKATGEVLKNAKNLAITANREFDYQTKLMEGTNEFTFSFTPDKNYPPEEYVEM
                     TSYETKEILHTVDYRKPKYSTVYVTPEGSDAGNGSRQNPLSLTQALRYAYAGQTIVMA
                     PGTYSFERGLTIPKGVNGTRENPIQLIAEKNPENKRPVLDFKGTGNGMTLFSHYWGLR
                     GFDITNSAAMQKGLQISGNHNLIEEIHAYRNGNTGIQISGSSNDPFEAWPAHNLVKNC
                     TSFENADPGFEDADGFAAKLTIGEGNVFDGCIAYHNADDGWDLFAKNETGSIGKVIIK
                     NSVAYRNGWVPGIEGEGNGNGFKMGGSSLTGPHELINSLSFENLAKGIDSNSGTDIIV
                     NSSTSFNNGSYNVALYTSSAGNTDYHASGILSFRTENLEMREQIRPLNQNEDQIYHSL
                     NYYWNEREKQSENTLGHTISKDWIESLNTCSKEGQQPVTRYENGSIHVHGLMQIKPGN
                     RQTEKERVDGLPLSVGAIFDGETSPSSEYPEYAIVQEPNQTKPNEQNNPIIIQEANGV
                     AENAEGNIIELKPFTVFVSTFTPNQGTPVFEDMRYFPEKIQIVETQSSAQFSNHTAIF
                     SAPSKGEYTLEVTYRSEIFDGEKWSAHTESVTQQKAITVRETADVPPGNDKPERENPG
                     NESPNGKPNGKPSHPDRTAKESEKNGTQGLPKTGEFNNPLLALAGGILLIGVVVYVMK
                     QRKK"
     gene            complement(8473..10380)
                     /locus_tag="IXT20_001441"
     CDS             complement(8473..10380)
                     /locus_tag="IXT20_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M13 family peptidase"
                     /protein_id="HAQ4859895.1"
                     /translation="MNKERLKQDFYEAVNEEWQKTAKIPADKPATGGFQDLVDGIDQL
                     LMGETDDLLAHPEKVSSEKMKHFLAYYQLANDYDRRNELHAQPLQPIIEKVESLQSFD
                     ELNKQFPEWLLDTLPLPFNLDVDADMKNAQTNAFFAYPPSLFLPDKTYYASDHPNGAQ
                     LIGVFFDMAVQLLELAGKEKSQAEAITEQAVQFDKLIAPHVKSAEENADYSKMYNPQS
                     FDTFIKYTDKLDLKKLVTGLIGTTPDKVIVTDPVYFDHLNDILTEAHFPLFKSWMIIK
                     TVRSLSGYLSEEFRQVSGIYSRVLSGTDEAMPQKKAAYYLASGQFDQVVGDYYGKKYF
                     GEAAKKDVEQMVKKMIAVYKKRLEENDWLGDATKEKAVIKLNNLGIQVGYPDKIPALF
                     DQFKTVPADQGGTLLSNTLVFSKLTLKDRFSKWNKPVDRSEWEMSADTVNAYYHPFRN
                     VIVFPAAILQAPFYSLEQSSSANYGGIGAVIAHEISHAFDNNGALFDEYGNLNNWWTE
                     EDLTQFQNKAQAMIEEFDGLPFADGKVNGKLTVSENIADAGGLSCALEAAKTESDLSI
                     EEFFVNWATIWRTKAKKEYQQLLLQIDVHAPAKLRANVQPKNLDEFYETFGITEQDAM
                     YLAPEKRVRIW"
     gene            10510..11385
                     /locus_tag="IXT20_001442"
     CDS             10510..11385
                     /locus_tag="IXT20_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide dehydrogenase"
                     /protein_id="HAQ4859896.1"
                     /translation="MKKRRRSQLNQVVDKPLYFKVDKKIKKLASTQQLQSRKSKLLFL
                     ALVFEDQSYVIIDQSGHPVEYSPAKYTYQEGLSCKKWKLINEEPIELSRWINRKEDIP
                     VLIEEKRSGKELANCWVGLPEERFLRYKKWATPSGYLCGTYAAAVLLAYYQDYRKGWM
                     LPNEIRKKNTADSLVLTKALRSQIQPLGLPTIPFQVSTGISSFLKKNGNHERARATLL
                     GSWQRATKRIREGKPVMIGILKVLGSTYGNHWVTAYAYFETETGERYYKVHDNWGDYH
                     KVIPASWSNGTVSLP"
     gene            11465..12439
                     /locus_tag="IXT20_001443"
     CDS             11465..12439
                     /locus_tag="IXT20_001443"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HAQ4859897.1"
                     /translation="MSNKYQDDTLKIFSLNGNRPLAEKIAKVFGTELGKSVVKQFSDG
                     EISINIEESIRGDHVYIVQSTNAPVNDYYMELLIMIDAMKRASAKTINVVLPYYGYAR
                     QDRTAKPHEPITAKLIANLIEEAGATRVLTLDLHTVQVQGFFDIPVDNLFTMPLFAHY
                     YRQLGLTGDDIVVVSPKNSGVQRARSLSEYLNSTLAIVDHADDASTEGGYVIGDVQGK
                     TCIMVDDILNTGATLARAANVLKENGAKEVYACASHGLLSDHAKSILDNAPIKDICIT
                     DSVFTEENRHPENLTIVTCSELMGEAVKRIHENTPMSPLFRLEEKEFE"
     gene            12628..13764
                     /locus_tag="IXT20_001444"
     CDS             12628..13764
                     /locus_tag="IXT20_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HAQ4859898.1"
                     /translation="MKTIYLDHAATTPMHPQVIEAMTAIMQDTFGNPSSIHGFGRHAH
                     EKLETARQIIADSLQAKPHEIIFNSGGTEGDNTAILETAFSRQKEGRHLITTAIEHPA
                     VLNTMKYLETKGFDVTYLPVDEKGNLSIEEVKKALREDTILVSVMYGNNEIGNLMPIK
                     EIGELLADHPAYFHTDAVQAYGNQTIHPEELGIDLLSISAHKINGPKGVGFLYKRDGV
                     NIPALLLGGEQEEKRRAGTENLAGIWGMAKAVELLSPEEREARNNKYKGFQQLILDTL
                     EANEIPYQVNGDLTNKLPHVLNLRLIGEANDLLLMKLDLNGVAISTGSACTAGNIEPS
                     HVLEAMYGEDTPILKESIRISFGLGNTEDDIQYFLDQLVRAVKK"
     gene            13901..14245
                     /locus_tag="IXT20_001445"
     CDS             13901..14245
                     /locus_tag="IXT20_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1831 domain-containing protein"
                     /protein_id="HAQ4859899.1"
                     /translation="MAFETTATVLGAPISYRLHPDAKRYTLRDNGFTETNGGNYQLIR
                     PLDATPQSKEGFKLKITVSKDIQTLKMSITTANGLKAVNIFKDPKQKMSQDKFYFLMD
                     GMISRGLFEKVE"
     gene            14516..15802
                     /gene="efmA"
                     /locus_tag="IXT20_001446"
     CDS             14516..15802
                     /gene="efmA"
                     /locus_tag="IXT20_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EfmA"
                     /protein_id="HAQ4859900.1"
                     /translation="MENEQSVVLTNWKRNYLFFLSGQFLSGITSMVVQYAIIWYLTRE
                     TGSATILSFATLLGMIPMVLLSPFVGPLVDRWDKKALLIVTDIIVAIFALILAVVGTI
                     SESFPIWLVFVSLFMRSVAQTFQMPTIQSIMPTIVPSSHITRTNGQLGMVQSANFIIA
                     PALGAALFSVVPVNYLILLDVLGAVFGVGLLIFVKIPKVSPEILEVPLTIFKDAKFGL
                     QQLMDNKGLWYITINGAFVMLLFMPAISLYPLMTLDYFGGSVGQAGAVEVVYAVGMLL
                     GGALISFIGTWKDRMKPIIIAYIIMGLTIGASGLVPNDSQGFLYFLILNAGAGCATPY
                     FNTLLMAMIQQSYESNVLGRVLGNFNSLMNLAGPIGLLFAGPLADRLGVEKMFLFSGI
                     GILLCGIVLFLTSAARNYDKELQKKLVKEHHEQKDE"
     gene            complement(15940..16539)
                     /locus_tag="IXT20_001447"
     CDS             complement(15940..16539)
                     /locus_tag="IXT20_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphatase"
                     /protein_id="HAQ4859901.1"
                     /translation="MNYFTSDMHFFHERLLGKNDFAPRLFANVDEMNQQLITSWNAVI
                     KETDHVYHLGDLAMHPQYEKANAEILELVTQLQGTIHLIKGNHDSRAFFRYLEKNDPR
                     LTDGSPKFYFYDVGAIVKFDHQQYYLTHYPMLLGPNDTIRNLHGHIHHYSVPIENDVN
                     VGVDAPERELLKEKLPFGTPLSEQQIKEIYEAKRKYDRS"
     gene            16943..18064
                     /gene="mnmA"
                     /locus_tag="IXT20_001448"
     CDS             16943..18064
                     /gene="mnmA"
                     /locus_tag="IXT20_001448"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HAQ4859902.1"
                     /translation="MTDNSKTRVVVGMSGGVDSSVTALLLKEQGYDVIGIFMKNWDDT
                     DENGVCTATEDYKDVAKVAAQIGIPYYSVNFEKEYWDRVFEYFLAEYRAGRTPNPDVM
                     CNKEIKFKAFLDYAMDLGADYVATGHYAQVTRDENGVVHMLRGVDNNKDQTYFLSQLS
                     QEQLSKTMFPLGGMEKSEVRAIAERAGLATAKKKDSTGICFIGEKNFKQFLSNYLPAK
                     KGNMVTLDGEVKGQHAGLMYYTIGQRQGLGIGGGGDSQEPWFVVGKDLATNTLYVGQG
                     FHHPALYATSLDASEIHFTTNEPMPKEFKCTAKFRYRQQDVPVTVRLLDDNHAEVVFD
                     EPVRAITPGQAVVFYDEMECLGGGLIDHAYQETKVLQYV"
     gene            18310..19731
                     /gene="arcD"
                     /locus_tag="IXT20_001449"
     CDS             18310..19731
                     /gene="arcD"
                     /locus_tag="IXT20_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine-ornithine antiporter"
                     /protein_id="HAQ4859903.1"
                     /translation="MEKKSTGISKWGLVALVVSSSIGSGVFGITSDLASSAAPGPAIL
                     SWIIVGIGILALVLSLNHLGEKRPDLDGGIFGYAEASFGKLGGFISGWGYWLSAWLGN
                     VAFATMLMSAMGEFFAIFKGGQNVPSILFASIFIWGLTLLVNNGVEGASVINTIVTIC
                     KLVPLFLFLIFVFIAFKVNIFTADFWGNVSDNLVNGTGQQGTIWLQIKGCLMVMMWVF
                     VGIEGASVLANRAKKRSEAQQASIIGLLCLLFIYILASILPYGVLSQEELATISQPAM
                     ANILKGIVGNWGAALINIGLIISIIGSWLSWTMLPAETTMLMARDGLLPKGWGKTNKK
                     NAPTYSLVVTAILTNLFLLTFLVTDYAYQFAYSLCTAAILICYLLVGIYQVQFSLKEG
                     IKKQVVIGGIAVAFELMGIILAGFSYVLLCSIAYLPGFCFYWIACRETKHQITGKEKV
                     MIGLICVAALLSIGLLAVGWIKI"
     gene            19745..20863
                     /locus_tag="IXT20_001450"
     CDS             19745..20863
                     /locus_tag="IXT20_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HAQ4859904.1"
                     /translation="MKIANFGVEEWLNKWEKKAVYDIAQSSIEALTLDELVGLDGTSV
                     SEFFEKRKNEPLDYGWIEGSDVFKQEVASLYQTVDPENILQTNGATGANLLALYALVE
                     AGDHVVSMLPTYQQLYDIPKSLGAAVDFVHLKEEEDWQFDLEQLEALVKPETKIICLN
                     SANNPTGTLLDRKTLEKIAEIARTVDAYVLIDEVYAPLTDKGEFLSIVDVYEKGIATN
                     SLSKTYSIPGIRIGWTATGPELAEVFRKYRDYTMICGGVLSDDLAVHALKNKEKILER
                     NKKIITENLVILKQWVANEPKVELVAPNYVSTSFIKLAIEEDDQTFCINLLEETGVLL
                     VPGSAFDLPKHARLGYCCKKETLEKGLFLLSEFLKKQA"
     gene            complement(20923..22215)
                     /locus_tag="IXT20_001451"
     CDS             complement(20923..22215)
                     /locus_tag="IXT20_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HAQ4859905.1"
                     /translation="MAARIKEKLFRKRETWEQNLFVLWFGTFMAGIGFSLVMPFMSLY
                     IDTLGNYSTSQLNFWSGLTFSSTFLVTTLISPWWGKLAHQKGRKLMLLRASLGMAIVI
                     SLMGCVTSVYQLVALRLLQGIFSGYISNAAALVATGTPKEKSGQVLGTLATGSVTGTL
                     LGPLLGGISASAFGYRPTFFITGIILFFVFLLSLFFVHEKFVPVKKENMVSAKQIFKD
                     LKYPHVVIGMFVTTMIIQASNNSISPIISLYIRQLLHGHGNVTLISGIIASIPGIATL
                     IAAPRFGRLGDKIGSERILAIGLGFAILVYIPMAFVTNVWQLAALRFLMGISDACLLP
                     AVQALITKYSPHQAAGRIFSYNQSFQATGNVVGPMIGSSVSSAFGYRGVFLSTSLLVL
                     VNFLLVRHNTQEIHQQETAEEKASGVSISSGHSPSHRQ"
     gene            22365..22925
                     /locus_tag="IXT20_001452"
     CDS             22365..22925
                     /locus_tag="IXT20_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAQ4859906.1"
                     /translation="MKKRNLSQEKIIDCFRELAEEMDVQQITFQHLAKRLDIKAPSLY
                     NHFKNIRDVKTALTAKLLQELNDQLRRALVGKSGQEALQIYAETYRSFAFSNQAVYEL
                     LISVPHTNEAVLLEGIHETNQIILQIFEAFQLTKEEKVHRSRELCSMIHGYLSLRFLG
                     YFTKESTIDPEDSYFWMINDFIATLP"
     gene            23054..23431
                     /locus_tag="IXT20_001453"
     CDS             23054..23431
                     /locus_tag="IXT20_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1033 family protein"
                     /protein_id="HAQ4859907.1"
                     /translation="MYQVITMFGDNEPWWFFEDWEEDIEAEETFESFEEARQAYEKQW
                     NEIHQNYEYINAKSNFLSAFWNDGDERWCEECDDDLQQYKGLALLKNHQPITVESGKE
                     FYETTNSSGKTKRCERPKQGAWC"
     gene            23364..25436
                     /locus_tag="IXT20_001454"
     CDS             23364..25436
                     /locus_tag="IXT20_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HAQ4859908.1"
                     /translation="MKQLILAEKPSVAKDLSKVLGANQKHKNYYEGPKVIVTWALGHL
                     LGLKMPEDLNKEWQSWQMDTLPMIPKKLGIKPLPKTGHQLKAIKQLAQRKDVSEAVIA
                     TDAGREGELVARWILEYVHFNKPVKRLWISSQTDKAIKTGFKQLRPAKEYDALYESAL
                     ARAKADWLVGLNVTRALTVKYADNLSAGRVQTPTLALVRDQEKKIEQFRPQTYFTITL
                     QAGKEQAKMLQKNQFALKTQEEAQKLVQLISTSKGTVTEVQEKLKTESAPLPYDLTEI
                     QREANQRYGYSAKKTLSLVQSLYETHKIVTYPRTDSKYLTNDMKSTMKERLQAVSDFA
                     PEVKQYLKNGAIVQQTKVFQDSKVTDHHALLPTENRARYEKLSNEEQKIYQMIVSRFL
                     GLFAQPHKVSQTKVTVEFGKERFIFRQNRVVQAGWKGETETETETVKWEKEMRINPDF
                     TIKKELSAPPKPLSEASLLGQMEKYSLGTPATRAEIIEKLIKSELMERTPQGLQVSPK
                     GKQLLVLVNPSLVTPDLTESWEKDLEAIAAGKKQAGTFLNGIEKETKRLVNEIKSSKQ
                     EYQDFSITQKKCPECGANLREKNTRDGKIYVCTNSDCNYRRRKDPKVSNHRCPQCHRK
                     MEIIEGKNGAYFRCKYDGTTEKMLDKKEQKKKMTKHEERRLLKKYAQAEEPEESPLAA
                     ALKAAMKK"
     gene            complement(25647..26594)
                     /locus_tag="IXT20_001455"
     CDS             complement(25647..26594)
                     /locus_tag="IXT20_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAQ4859909.1"
                     /translation="MKVSIVVPCFNEEKTVPLFFDEVEKFKGAHEFEYLFIDDGSKDG
                     TLAAIKKLASAYPYVRFVSFSRNFGKEAALYAGLQASTGDLVTVMDVDLQDPPELLPE
                     MINRITTSDVDSVGTRRSTREGEPAIRSFFAKKFYQLINKISDTEMVDGARDFRLMTR
                     QMVDAVLELTEYNRFSKGLFSWVGFKTEYISYENRERVAGETSWSFWKLFNYSIDGIV
                     NFSDAPLTIASFIGALSCVGSGIAILFIILRALLFGDPTSGWPSMVSIFLFIGGIQLL
                     CLGIIGKYIGKIFLETKQRPVYIIRETEKVKPNELKPHQ"
     gene            26839..27303
                     /locus_tag="IXT20_001456"
     CDS             26839..27303
                     /locus_tag="IXT20_001456"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HAQ4859910.1"
                     /translation="MKIHTPIGFLWLDADESGLTAVSFQPISESESANQQILTRTQEQ
                     FEEYFAGKRKEFDVPLSIKRGTPFQQNVWKALSTIPYGEIRTYKEVAVAVDSPKAVRA
                     IGQANRTNPLPIVIPCHRVIGQNGKLTGYMGNAIDGVEIKRQLLLLEGYLQN"
     gene            complement(27365..28408)
                     /locus_tag="IXT20_001457"
     CDS             complement(27365..28408)
                     /locus_tag="IXT20_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HAQ4859911.1"
                     /translation="MKSLSHPIKSVKDSAVREILPGLLLSFAIAAFSRLLAIYLPQLG
                     GATIAILLGILLGNTFFKQPVLAIGTKFAESKLLEFSVFLLGATVTAQTIAQIGIKGI
                     VFVLLQMSVTIIGAYLIGKKLQFSDTSSLMMAGGNAVCGSSAIASIAPSIHAKEEEKG
                     QIITLVNLLGTVMMLTLPFLGLQLFGGNLIEKSALLGGTLQSVGQVVAGASLLDNSVV
                     EFAMLFKILRIMMLVVVVYLFEKFMHKKQPALFETPTDTQKKRKLPLPWYVLGFVIFC
                     LVNSLFSLPVVIDQSAHFISSWFEITALAAIGLRLDFAKFMKEGTRFLSYGLAVGLLQ
                     TVMALLFIWLIKV"
BASE COUNT         9160 a         5218 c         5993 g         8158 t
ORIGIN      
        1 aaactgttgt tgggcgtttg ttttagtaac aagcgaaagg tgatctattg ggttaaatcc
       61 aacagttttt gtttcagatc ttcttccatg tgtccaagct ctacttctgc atgacgacgt
      121 ttttcttttc cttctttttg gatacgcaat gtctcttgga ttgtttcgat caaatcattt
      181 tgagtggttt gaagggtctc gatatccacg attccacgtt cattttcttt cgctgtttcg
      241 atggctgaga ttttcagcat ttctgaattt ttcttcaata agtcgtttgt tgtttcagag
      301 acttggcgtt gagcagttac tgcatctttt tgacgaagca aggtcaatgc gatcacgact
      361 tggtttttcc acagcggaat ggctgtagcg atagatgctt ggatcttttc agccaaggcc
      421 tgatttgtgt tttggatcaa acggatttgt ggtgcttgct ggatcgtgat ctgtctagct
      481 aagcgaagat catgcgtacg tttatccaga cgatctagga attgcgtgta atcattggca
      541 atctgaacat ccatctgatc gccagtttct tctgctcttt tcatcgcttc tggaatgatc
      601 gttgtttgaa gttcttccat tttcaattct ccagcagcga tataaatatt cagtgcatca
      661 aagtagtctt tgtttttctg gtaaagctgt tctaacatca gattatcttt tagcagaccg
      721 tctttttctt tatctaattt cactgaaatc ttgtcaattt gtgcaccaat cttttgatat
      781 tttgctgtca cttcatagat cgattgtttg accttcccga agacacgctg gaagatgttc
      841 ccttctccgg cacgcagttc gtcaggattg gcttcttgga gacgatacat cagttcagtt
      901 aaggaatctc ctacaggacc aatatcttgt gcttgcacat gattcaacat cgattgtgag
      961 aattcactta attttgcttg ggcagccgat ccgtagctga tgacagactg tgcatcatta
     1021 gcatcgattt ttgctgctag ctgtttagct tgttcttggc gttccgccgg tagtttgtca
     1081 attaaacgag cagctgtctg ttgctcttgg agctgatcaa tctccgcttg ttgtgaagtg
     1141 gtcagcgtat ctgttggagt tgaaaacggg ttgttcaata aatcttcaag tgtatcgtta
     1201 actggtgtaa catcttttgt ttcagtcggt ttatgatcca taaaaaattc ctccttgttg
     1261 aatctgttct cgattcttac atgaatgtct ctactttaga aaaagtactc gaaacagcca
     1321 gacatcatct gaatgttccg agtcttcttt tatgaacaaa agagttaatc cttttgttct
     1381 ggttcatatt tattatcacg ctttaagctc tgtttcgcaa cagatatctc tacgtcgata
     1441 tcatctagat cctcagcaac gaatttttga taatcttgtg cgattaaagc tgacagctgg
     1501 tcgatgatct gcgcgctttc ttctagtttt tcataggtct gtttgttttt gatctcatgg
     1561 tcattgatct cattatactt atttgtcaaa tcaactagat tcggcagatg cgtataaaga
     1621 aactgacttg cttcatgcag tcggtttggt tctttcacca attctttgaa taaagctttc
     1681 gctgctttga ctggatcatg gcggagatcg attgctttaa gtttggctgt ctgctgcata
     1741 ttcatttgca attgattgat ctcatttttt gtttgattca ttgtttgtcg aaaaaatgaa
     1801 atttcattcg ctgtcatacc gcttttttca tagtgagttt caagttcgtc agtcagtgca
     1861 ggaatttctt cttgttttcc ctttttcctg cgcgaaccaa agagtcccca caaaactaaa
     1921 actacaccta tcaataaaag tcctagaaca aaagaaaaac tattgtttcg gacaaagaga
     1981 aatagcaaga ccaatgctag aagccacggc caatttcgtt ttatcataat aaaccctcca
     2041 tgtcatttat cgttgagtat tgaattcttt tcgttattcg attacatact tattttagca
     2101 tacggactac tagaaagagt atcggactaa agaaggattt tctatattta gagaaaatct
     2161 aaggtttatc tatggtaaac taataaggag tttttgagaa aaattgaaaa atgaaaaaaa
     2221 agcagttatg attacaaaga aagcaggaga agatatgctg aacaatgagc aatttgaaga
     2281 aaaaacaatc agtcgcaaag aaatctatag cggaaaaatt attgatgttg ctgtagacga
     2341 agttcgtttg ccagatggag gaacttcgaa gagagaactg gtatttcatc ctggcggtgt
     2401 cgggatcatt gcttttgacg aacaagatcg tctgcttctt gtcaaacaat ttcgaaaacc
     2461 tttagaaaaa gtgattttgg agattccagc tggaaaaatc gatccaggag aaggacaaaa
     2521 tcccgaaatg acagcagcta gagaattaga agaagaaact ggctatcgtg cgaagtcgct
     2581 gagccatctt acttcgatgt atctttcacc aggttttgca aatgaagtcc ttcatattta
     2641 tcatgcacaa ggggtagaga aggtcgagaa tcctttagca caagatgaag acgaagtact
     2701 ggagttgtat catctaactc ttgaagaggc gcaacaagcg atgaaagatc agttgatttg
     2761 tgatgcaaag acgatttatg cgattcaata ctgggaacta ttgaccaaag gaaagtagag
     2821 ggatcggctt atggccaaag gaccgcttat tacgcgaagc gaacttcgca agcgacaaca
     2881 agcacaagca agcgagtcat taaaaaaaca gcgaaaagca gaaacagctt atcagcaaga
     2941 agaaaaaaag attgccagct tttatcgtaa agaaagcaaa aagaataaac caatcacgaa
     3001 aacaaggatc agcgaacggg aaaagacgac caaatggaac tcttttttga tgaaatctct
     3061 tattattgtt atcctaatgt tatgtgtggt ttttttagca atcgcattta tatagaaaga
     3121 aggaattaaa aagatgaaaa tcggtattat cggcgcaatg gaagaagaag taaaaatttt
     3181 gagagagaac ctaagcgaac cattatcatg ggaacgcgca ggcgctttat ttatttcagg
     3241 ttcattaggt aaccacgaag tgatcgtcgt acgttccggt ataggaaaag tcttagcatc
     3301 tattaccaca agtctgttga tccaacaata cggcgtgaac atggtgatca atacaggttc
     3361 agctggcgga atcggtgaag gcttacaagt aggtgacatc gttatatcag acaaagtagc
     3421 ttattttgat gcagatgcaa caggatttgg ctacaaccca ggacaattac caggaatgcc
     3481 tctatattat gaagcaagca catatttgcg cagtgaaatg gttaaagcag caaaagcaac
     3541 gaatttgaac gcaaaagaag gattgatcgt tacaggtgat acatttgtcg actcgccaga
     3601 taaaatcaaa gaaatcttaa cgaatttccc tgaagcatta gcttgcgaaa tggaaggagc
     3661 cgctgttgga caaacagctc gtcagttcaa tatcccattt ttgatcgtac gtgcaatgag
     3721 cgacacagca gatcactcgg cgacacaaag ctttgacgaa tttatcgaag acgcaggcaa
     3781 acgatcagca gaaatggtga tcgaatttgt caaacatctc gtataaggag gactaagatg
     3841 aacgcattga tctcgattga ctacacatat gattttgttg cagaggatgg aaagctgact
     3901 actgggacag caggacaagt aattgaaaaa aagctgactg ctcatacaaa gaagtttatt
     3961 gatcaaaagg attttgtcgt ttttgccatt gatgcgcatg atccaaaaga ttcctttcat
     4021 ccggagaacc gcttatttcc tccgcataat gtgataggaa cgggtggacg ggaattgtac
     4081 ggctctttaa aaagtttata ccaagactat gagcaagagg aaaatgttta ttggatagac
     4141 aaacggcatt attccgcttt tagtggaaca gatctagaca tccgtttgcg agaacgccag
     4201 atcacagata tttatttgac cggcgtatgt acagatatct gtgtgttgca tacagcagtc
     4261 gatgcgtata atctaggata caaactccat attttcaaag attccgtggc aagtttcgat
     4321 cccgttggac atgaatgggc attgagacat tttgagtcca cactaggcgc agaaatttta
     4381 taaaaaaaca aagtgatttg ataaaataaa aagcacaaaa taataaaaga agagcataaa
     4441 acaaacgact ttgttttatg ctctttttta tacgttattt taaaaaaact tattcttttt
     4501 ccgttaataa atctctttat tatcaacaac ttaagttata ttttttcttt ttataaaata
     4561 cacttattat aattttgttt tttattgaca gtaaaaataa attaggtaat actttaagta
     4621 aacgctttct aatttgttac gggaggaata cacttgttaa aaaaacgtat aaaatacagt
     4681 gcgctaatga tgaatctcct tatgctcagt accccggttt taatttctgt cgttgaagtt
     4741 gctgctaacg aacaacagtc tgtgaacgaa gaaaatgcac acgtgaacat tgaagggatg
     4801 tcaacaatgg aggaaatacc aagaactagt gaagatatgg atacggaaga aggaaatgat
     4861 cctatgatga aagaatcaga aaatgaaaag acaaaaaagc aatctgaaga agcaaatgaa
     4921 gagtggaaag gggttgtttt cggagaaagc acaagtgcta gtaggtatgc cattgaacca
     4981 ttagaagacg gcgtgcggct ctcttccacg aataacggcg gaaaattcca gtcaagcgga
     5041 tcagatggaa tgacctatta ttatcaagca atccccaaag acatcaactt taggatgcgg
     5101 gcgacgatcg aaatagaatc ttggacctat acgaatgccc aagaaggatt tgctttaatg
     5161 gtaagagacg cagtaccaaa aaatcatttg tttgggcagg cattttattc aaattcattt
     5221 gccatactag gcagtcggat cgaatatgtc tgggactcgg atagacaaga agtggtcaac
     5281 acgagcggca gcaaatatac gatgcgactt ggattgggga cacgggcaat cacaggaata
     5341 tcatctgaag atccgcaagc tgcgccagct cctgggagtg tctcagtgga aacaacacct
     5401 ttagagactt cagcagggtt cttggaaaaa gaaacaggtt cttataatat attcggaaac
     5461 ggtcatggga atctgtttcc ggccacgaac tcgattacta aactggacgt agagatgaaa
     5521 aaaacgaata ctggtttaga ggcatattat tacgatcctg aaacaggaga agaaatggct
     5581 tctgacatca tgtatgactg ggaaaaatta tacgcttcag atgaatcggt tatttatgca
     5641 ggatttgctg cagcaaggaa tatgacgatc aaggtagaag gaatcgaagt tgcttactca
     5701 gatcctgcga cagatcctcc tgggcaagaa agacctactc gcttgattga tccgatttac
     5761 actgtgacct ctagtaatca ttcaggaaac caagatcata ctatgtcctt tagagcaaat
     5821 gctgacggat tattgacaat taaaaataaa gcaacaggag aggtgcttaa gaacgcaaaa
     5881 aacttggcta tcactgcgaa tcgggaattt gattatcaga caaaactgat ggaaggaacg
     5941 aacgaattta ctttcagttt tacgccagat aaaaactatc cgccagaaga atacgtagag
     6001 atgactagtt atgaaacaaa agagattctt catacagtgg actaccgtaa acctaaatat
     6061 tcgacagttt atgtaacacc agaagggtca gatgcaggaa acggaagcag acaaaatcca
     6121 ttatctctga cacaagcatt gagatacgca tatgctgggc agacaatcgt catggcgcct
     6181 ggaacttatt ccttcgaaag aggattgacg attccaaaag gcgttaacgg gacaagagaa
     6241 aacccgattc agttgattgc agagaaaaat ccagaaaaca agcgtcctgt acttgatttt
     6301 aaaggaactg gaaatgggat gacactgttc agtcactact ggggtttacg agggtttgac
     6361 attacaaata gtgcagctat gcaaaaagga ttacagattt ctggaaatca taatttgatc
     6421 gaagagattc atgcttatcg gaatggaaat acaggaatcc aaatcagcgg tagctcaaat
     6481 gatcctttcg aagcatggcc cgcgcataac ttagtcaaga actgtacatc ttttgaaaat
     6541 gccgatccag gatttgaaga tgccgatgga tttgcggcca aactgacaat aggagaagga
     6601 aatgtatttg acggttgtat cgcgtatcat aatgcagatg acggttggga cctttttgct
     6661 aaaaatgaaa caggttcgat cggaaaagta atcatcaaaa actcagttgc ttatcgaaac
     6721 ggctgggtac caggaattga gggagaaggt aacggtaatg gattcaaaat gggcggttct
     6781 tctttaacgg gccctcatga attgatcaat agtttgtctt tcgaaaatct tgcgaaagga
     6841 atcgattcca atagcggaac agatattatc gtcaatagca gtacaagttt taataacggt
     6901 tcatataatg tggctcttta tacctcttca gcaggaaaca cagactacca tgcttctggt
     6961 atcttatctt tccgaacaga gaatcttgag atgagagagc agatccgccc attgaatcaa
     7021 aacgaagatc aaatctacca ctcgttgaac tactattgga atgaacgaga gaaacaatca
     7081 gaaaatactc ttggacatac catatcaaaa gattggatcg agtctctaaa tacatgttcg
     7141 aaagaaggtc agcagcctgt tacgcgctat gaaaatggaa gtatccatgt tcatggactg
     7201 atgcagatta aaccggggaa tcgtcaaaca gaaaaggaaa gagtggatgg tcttccatta
     7261 tctgtaggtg ctatttttga cggagaaacc tcaccgtctt cggaatatcc agagtatgca
     7321 atcgtacaag aaccaaatca gacaaaacca aatgaacaaa ataatccgat tattatacaa
     7381 gaagcaaatg gtgtggcgga gaatgcagaa ggaaatatca ttgaactgaa gccttttaca
     7441 gtttttgtgt caactttcac accaaatcaa ggaacgcctg tctttgaaga tatgcgttat
     7501 ttcccagaaa agatccaaat cgttgaaaca cagtcaagtg ctcaattttc taatcatacc
     7561 gctatttttt cagcaccttc aaaaggagaa tatacgctag aagtcactta tcgttcggaa
     7621 atatttgatg gggaaaaatg gtctgctcat acagaatctg ttacccagca aaaagcgatc
     7681 actgtgcgag aaactgccga tgtgcccccg ggaaatgaca aaccagaaag agaaaatcca
     7741 ggaaatgaat caccaaatgg aaaaccaaat ggaaaacctt ctcatccgga taggacagca
     7801 aaagaaagtg aaaaaaatgg cactcaagga ctcccaaaaa cgggagaatt taataacccg
     7861 ttgctagcat tagctggagg aattttgtta atcggtgtag ttgtttatgt catgaaacag
     7921 cgaaaaaaat aagtggaatt tctcctttta tgatgtaaaa taattgactg aggaaagtaa
     7981 aaatagaagg aaatgagacc aatagatgtc tcacttcctt ctatcttttt atagatgagg
     8041 ttctccttgc tgcttttgat tggctgttct tattacttga tgcaggacag ctttttcaca
     8101 atctctccac taaacggtgt tcaatcagct gaccctcggt attagctcaa caaacggcaa
     8161 aatatgagga actatttttc cgtttttttc tctaatactc aggtcaggac gctgatttca
     8221 caacctctcg ataaacggtg ttcaaaagcc agtttctcgg tagtaagtca aatttatgca
     8281 aaaatgtgga aagccatttt cacaaaattt ttcttactac ttgaaactaa gcagcttttt
     8341 cacaacctct aatacatggt attccagaag taacttcttc gaaaaataag ccgaaatgtc
     8401 acaaaaattt gagaagcgat tttcccaaat tccttcttat ttcttgaagc tgaccacttc
     8461 tggcacaacc tcttaccaga ttcgcactct tttttctggc gctagataca tagcgtcttg
     8521 ttccgtgata ccaaatgttt catagaattc gtcaaggttt tttggttgca cgtttgctcg
     8581 gagtttagca ggggcatgga catcgatttg cagtaaaagt tgttggtatt ctttttttgc
     8641 ttttgtacgc caaatcgttg cccaattgac aaagaattcc tcgatagaaa gatctgattc
     8701 cgtttttgct gcttccaatg cacagcttag accgcctgca tcagcaatat tttccgaaac
     8761 ggttaatttt ccgtttactt ttccgtcagc aaaaggcaga ccatcgaatt cttcgatcat
     8821 tgcttgtgct ttgttttgaa attgtgtaag atcttcttct gtccaccagt tgttcaagtt
     8881 gccatattcg tcaaataaag cgccgttatt atcaaatgca tgggaaatct catgagcgat
     8941 aacagctccg attccaccat aattagcaga gcttgattgt tctagactgt aaaatggtgc
     9001 ttgaaggatc gctgccggaa agacgatcac attccgaaat ggatgataat aagcattgac
     9061 cgtgtcagca ctcatttccc attctgaacg atccactggt ttgttccatt tgctgaaacg
     9121 atcttttaat gtgagtttgc tgaaaacaag agtatttgaa agcaaagtac caccctgatc
     9181 tgcaggtact gttttgaact ggtcgaataa tgctgggatc ttatcggggt agcctacttg
     9241 gatacctaag ttatttaatt ttatgacggc tttttctttt gttgcatccc caagccagtc
     9301 attttcttct agacgttttt tataaacggc gatcattttt ttcaccattt gttctacgtc
     9361 ttttttagca gcttcgccaa aatatttttt tccgtagtaa tcgccaacta cttgatcgaa
     9421 ttgtccagag gcaaggtaat acgctgcttt tttctgaggc attgcttcgt ctgttccaga
     9481 caatacgcga gaatagattc cgctgacttg gcggaattct tctgatagat aaccactaag
     9541 agaacgtact gttttaatga tcatccagct tttgaataac ggaaaatgag cttctgtcag
     9601 gatgtcattc aaatgatcga agtacactgg atcagttacg atcactttat ctggagttgt
     9661 cccaatcaaa cccgtgacaa gttttttcaa gtccagttta tctgtgtatt taataaatgt
     9721 atcaaaagat tgcggattat acattttgct gtaatctgcg ttttcttctg cactttttac
     9781 atgaggagca atcaatttat caaattgcac tgcttgttcc gtgattgctt ctgcctgact
     9841 tttttctttg ccggcaagct ctaataactg aacagccata tcaaagaaaa cgcctattag
     9901 ttgtgcaccg ttgggatggt ctgatgcata atacgtctta tctggaagga ataaagaagg
     9961 cggataagca aagaaggcgt tcgtttgcgc attcttcata tctgcatcta catccagatt
    10021 aaatggcaat ggtaatgtat ccaacagcca ttctggaaat tgcttattca attcgtcgaa
    10081 agactgcaat gattcaactt tttcgattat aggttgaagc ggttgagcat gcaactcatt
    10141 tcttcggtca taatcgtttg ccagttgata atatgcaagg aaatgtttca ttttttctga
    10201 agacactttt tctggatggg caagcagatc gtctgtttct cccatcaaaa gttggtcgat
    10261 cccgtcaact aaatcttgga aaccgcctgt agctggttta tctgcaggga tttttgctgt
    10321 tttttgccat tcttcattga ctgcttcata gaaatcttgt ttcaatcgtt ctttgttcat
    10381 tcatcgatcg ctcctttttt gtttcgtgtg cccattatct tacaaaatga aggaattaac
    10441 aaatagaaca taaaaaattg aaaattcgaa caatcaaact gatttgtttt tttcaatcct
    10501 tcacttataa tgaaaaaaag gaggcgatcc caactgaatc aagtagtgga taaaccttta
    10561 tatttcaaag tagataaaaa aattaaaaaa ttagccagca ctcagcagct gcagtcaaga
    10621 aaaagtaaat tgcttttttt agctctggtt tttgaagatc aaagttatgt gattatcgat
    10681 caatcaggac atccggtaga atattctcca gcaaaatata cttatcaaga gggtctttct
    10741 tgtaaaaaat ggaagctgat caatgaagag ccaatcgagt tatctagatg gatcaacaga
    10801 aaagaagata ttcccgtttt gatcgaagaa aaaaggtcag gaaaggaact cgcaaattgc
    10861 tgggtgggat tgccagaaga acgttttttg aggtataaga aatgggctac cccctcaggc
    10921 tatctttgtg gtacttacgc ggcagcagtt ctccttgcct attatcaaga ctatcgtaag
    10981 gggtggatgc ttccaaatga gattcgcaag aaaaatacgg ctgattcgtt ggttttgacg
    11041 aaggcattgc gtagccagat ccaaccacta ggacttccta cgattccttt tcaggttagt
    11101 acagggattt ctagtttttt aaagaagaat ggcaatcatg agcgtgcacg agcaactctt
    11161 ctaggttcat ggcagcgagc tacaaaacgt atacgtgaag gcaaacctgt gatgattggt
    11221 attttgaaag tacttggcag tacatatggc aatcattggg tgacagcgta cgcttatttt
    11281 gaaactgaaa caggagaacg ttattacaaa gtacacgaca actggggcga ttatcataaa
    11341 gtcattcctg ccagttggag taacggaaca gtttcacttc cttaagaatt tgctgaaatt
    11401 aacagaaaat cttggaaaaa tatgttaaac tatcgaagaa tagagaagtt ttggggagga
    11461 ctacatgagt aataagtatc aggatgacac gttgaagatt tttagtttga atggcaaccg
    11521 tccattagct gaaaaaatcg cgaaagtgtt cggaacagaa ttaggtaaaa gcgtcgtgaa
    11581 gcaatttagt gatggcgaga tttccatcaa tatcgaagaa agtattcgag gggatcatgt
    11641 gtatatcgtt caatcaacga atgcacctgt caatgattac tatatggaac tactgatcat
    11701 gatcgatgca atgaagcgag caagtgcgaa aacgatcaat gtcgtattgc cttactatgg
    11761 gtatgctagg caagaccgca cggcgaaacc tcatgaaccg atcactgcga aattgatcgc
    11821 taacttgatc gaagaagcag gggcaactcg agtactgact ttagatctgc acacggttca
    11881 agtacaagga ttctttgata ttcctgtaga taatttattt acgatgccgt tgtttgctca
    11941 ttattaccgt caattaggtc tgacgggaga tgacatcgta gttgtttcgc caaaaaacag
    12001 cggtgttcag cgtgcacgta gtctttcaga atatttgaac agcacattag ccatcgtaga
    12061 tcatgcagat gatgcttcaa ctgagggtgg ttatgtgatc ggtgatgttc aaggaaagac
    12121 atgtatcatg gtagatgata tcttgaacac aggagcaacg cttgcacgag cagcaaatgt
    12181 attaaaagaa aacggcgcaa aagaagtata tgcctgtgcg tcacatggat tattgtccga
    12241 tcatgcgaaa agtattttag acaatgcacc aatcaaagat atttgtatca ctgattctgt
    12301 tttcacagaa gaaaatcgtc atccagaaaa cttaacaatc gttacatgtt cagaattaat
    12361 gggtgaagcg gtcaaacgca tccatgaaaa tacaccgatg agtccgttat tccgtctgga
    12421 agaaaaagaa ttcgaataaa caaaaaaaag agggaaagaa gttgtgacag aagtgagcag
    12481 cttcaaggag ctgcgacaag acttttgtca cagctccttt tttctatgcc catgaaacgt
    12541 cctctagaca aaaatgagct cttctttggt ttgttcgcta ttttatgcta gaatgtgcct
    12601 agcaataatt tggaagggat gaatgaagtg aaaactattt atctggatca tgcggcaact
    12661 acccccatgc atccacaagt aattgaagcg atgacggcaa tcatgcaaga cacgtttgga
    12721 aatccttcaa gcatacatgg atttggtcgc cacgcgcatg aaaaattgga aacagcaaga
    12781 caaatcattg cagacagtct gcaagcaaaa cctcacgaga tcatctttaa cagtggggga
    12841 acagaagggg ataatacagc tatcttagaa acagcgttct cgcgtcaaaa agaaggcaga
    12901 catctaatca ctacagctat cgaacatcct gctgttctta ataccatgaa atatttagag
    12961 acaaaggggt ttgacgttac atatctgcct gtagatgaaa aagggaatct gtcgatcgaa
    13021 gaggtcaaaa aagcgttacg agaagatacg atcttggtct ctgtgatgta cgggaacaat
    13081 gaaatcggaa atttgatgcc aatcaaagaa atcggtgaac ttttagcgga tcatccagct
    13141 tatttccata cagatgcggt acaagcgtac ggaaaccaaa cgatccatcc agaagaatta
    13201 ggaatcgatt tattaagtat ctctgctcat aaaatcaatg ggccaaaagg cgtcggtttc
    13261 ttatataaac gagacggagt aaatatccct gcattgcttt taggcgggga gcaggaagaa
    13321 aagcggcgtg caggtacaga aaatttagca gggatctggg gtatggcaaa agcagttgaa
    13381 ctgctgtcac ctgaagaacg tgaagcacgt aacaacaaat ataaaggatt ccagcaattg
    13441 attcttgata cattagaagc aaacgaaatc ccttatcaag taaatggtga tttaacgaat
    13501 aaactccctc atgtgttgaa tcttcgctta attggcgaag caaatgattt gctactgatg
    13561 aagcttgatc ttaatggggt cgctatttct actggttcag cttgtacagc aggaaatatc
    13621 gagccttcac acgtattaga agcaatgtat ggagaagata caccgatact aaaagaatcc
    13681 atccgtatca gctttggttt gggaaatacc gaagacgaca tccagtattt ccttgatcaa
    13741 ttagttcgtg ctgtcaaaaa ataactagtg aagtcattct tatatgaaca gatgtctgag
    13801 agaacgcctt cattttttag ggaacttaac tgaaaaacct agtaaaaatc aggataaact
    13861 tttataataa agagaaacta attttatcga ggtgaaaatc atggcatttg aaacaacagc
    13921 aacggtactg ggcgcaccaa ttagctatcg tttgcatcct gatgcaaaaa gatatacatt
    13981 gcgcgataat ggatttacag aaacaaacgg aggcaattat caattgatcc gaccattaga
    14041 tgccacgccg caaagcaaag aaggattcaa attaaaaatc actgtatcaa aagatattca
    14101 aacattgaaa atgtcgatca ctacagcaaa tggattgaaa gcagtcaata ttttcaaaga
    14161 tccaaaacag aaaatgagcc aagataaatt ttattttctg atggatggga tgattagccg
    14221 tgggttattc gaaaaagtcg aatgatctgt ggatataaaa agacaactag ttttgaaacg
    14281 gcctattgtt agatgactaa ctttgtaagg acgacgcaaa ttagttgtct tttttatttt
    14341 taaatttaac acatgttgtt atttttaata ttttgctgat ttattgtttc taagtaaaga
    14401 aatttaaatt gaaacgattg ttatttaggg aaaaaggtat tttttgaaaa aagaatcccg
    14461 tataatgatt gttgtgcaaa cgctatacaa aatgaaaaag aaaagaggga tgtttatgga
    14521 aaatgaacag tcggtcgttt taacgaattg gaagagaaat tatttgttct ttttatcggg
    14581 acagtttttg tcaggaatca ctagtatggt cgtccaatat gcgatcatct ggtatttgac
    14641 aagagaaacc ggttcggcga caattttgag ttttgctact ttactaggaa tgattcccat
    14701 ggttttacta agcccatttg taggaccgct agtggatcgc tgggataaaa aagctctttt
    14761 gattgtcacg gatattattg tagcgatttt cgcgcttatt ttagcagtag tcggaacgat
    14821 ttcagaatcc ttcccgattt ggctagtttt tgtgtctttg tttatgcgtt cggtcgcaca
    14881 aacattccaa atgccaacga tccagtcgat tatgccgaca atcgttccat cttctcacat
    14941 aacaaggacg aatggacagt taggaatggt ccaatcagcg aatttcatca ttgcgccagc
    15001 tctaggtgcg gctttgtttt cagtcgttcc tgtcaattac cttattttat tagatgtatt
    15061 gggggcagtt tttggtgtcg gcttattgat ttttgtgaag atcccaaaag tttctcctga
    15121 aatattggaa gttccgctta ctatttttaa agatgcaaaa tttgggctgc agcaattgat
    15181 ggataataaa gggttatggt acataacgat caatggtgca tttgtgatgc tgttgttcat
    15241 gccagcaatc agtctgtatc cattgatgac gctagattac tttggtggtt ccgttggaca
    15301 ggcgggagca gtggaagtag tctacgcagt cggaatgctc ttaggcggtg cactgatcag
    15361 ttttatagga acatggaaag accgaatgaa acctattatt atagcctaca tcattatggg
    15421 cctgacgatc ggtgcaagcg gattggttcc aaacgatagt caagggttct tgtactttct
    15481 tatcttgaat gcaggagcag gttgtgcaac gccatatttc aatacactgc tgatggcaat
    15541 gatccagcag agttatgaat cgaatgtttt aggtcgtgtc ttagggaact tcaactcttt
    15601 gatgaacctg gcaggtccaa ttggattatt atttgcagga ccattagctg atcgtttagg
    15661 ggtagaaaaa atgttcttgt tttccggaat cgggatttta ttatgcggaa tcgttttatt
    15721 tttaacgtca gctgcccgaa attatgataa agaattacag aaaaaactgg taaaagaaca
    15781 ccatgagcaa aaagacgaat agtatggaat ggagttgtga cagaagtctt gttacaactc
    15841 catttctgat cctaatgttc ctgtggcaat aagcctactc aaatgaaaaa tatgagtaat
    15901 tatctttatt tgtttaatac ttgatatagg gcagtttttt cacgacctat cgtacttcct
    15961 ctttgcttca tagatttctt tgatttgttg ctcagataac ggagtgccaa atggtagttt
    16021 ttcctttaag agttctcttt ccggtgcgtc tacgcctaca ttgacatcgt tctcgatagg
    16081 tacgctgtaa tgatggatat gtccatgaag attgcggata gtgtcgttag gcccgagcaa
    16141 catcggataa tgagtcaagt aatattgttg atggtcgaat ttaacgatgg cacctacatc
    16201 ataaaaataa aatttgggac ttccgtcagt aagcctagga tcattttttt ctaaataacg
    16261 gaaaaaagcg cgactgtcat ggttcccttt gatcaagtgg attgttcctt gcagttgagt
    16321 gaccagttcc aaaatttctg catttgcttt ttcatattgc ggatgcatag caaggtcgcc
    16381 tagatgataa acatggtcgg tttcttttat tactgcattc cagctagtga tcaactgttg
    16441 gttcatttca tccacatttg caaagagccg cggagcaaag tcgttttttc ctaataagcg
    16501 ttcatggaaa aagtgcatat cactggtaaa gtaattcatg tatcttttgc ctcccttttt
    16561 tcttagagta aacaaaatca cttatctgag caactcataa catctaaaaa gaaaaggttg
    16621 taaatgagac gtgtagcttt aagcgataag aagcaatttt gaaaaatagt ttttcacaat
    16681 ttataaaatt attccttatt gccgaaaggc tggccctttt tatgagtatg ccaatttttt
    16741 atatcgaatg ctcaaaagat gagtgatttt ttggttgact atcatgtttg tgtactgaat
    16801 aggtccagta gaacagtatt tttacgactt gataattaag ggatgaaatt ttcagaaata
    16861 tgacgtataa tgtaaaatac tgaaaagttg cgaccttcaa ctcgtagatg tttcagaatc
    16921 taattgaaat gatggtgata tcatgacaga caacagcaaa acacgcgttg tcgtagggat
    16981 gagcggcggt gtcgactcat ctgtaacagc acttttgtta aaggaacaag gatacgacgt
    17041 gatcggaata ttcatgaaaa attgggatga cacggacgaa aacggtgtat gcacggccac
    17101 ggaagattat aaggatgtag cgaaagtagc tgctcaaatc gggattcctt attattctgt
    17161 gaattttgaa aaagaatatt gggatcgtgt atttgaatat ttcctagccg aataccgcgc
    17221 aggacgtaca cctaacccag acgtgatgtg caacaaagaa atcaaattca aagcattctt
    17281 ggattatgca atggatcttg gcgccgatta tgtagctact ggacattatg cgcaagtcac
    17341 tcgcgatgaa aacggcgtgg tccacatgct tcgtggagta gacaataata aagatcaaac
    17401 ttacttcctt agccaattat cacaagaaca gctttcaaaa acgatgttcc ctctaggcgg
    17461 catggaaaaa tcagaagtac gggcaattgc tgaacgcgca ggccttgcga ctgctaagaa
    17521 aaaagattca acgggaatct gttttattgg tgaaaagaac ttcaaacaat ttttgagcaa
    17581 ctatctgcca gcaaaaaaag gaaatatggt cactcttgat ggtgaagtca aagggcaaca
    17641 tgctggcttg atgtattata cgattggtca acgtcaagga ctagggatcg gtggcggagg
    17701 agattcgcaa gaaccctggt ttgttgtagg aaaagatcta gcgacaaaca cattatatgt
    17761 ggggcaaggg ttccatcatc ctgcattgta tgccacaagc ttggatgcaa gtgaaatcca
    17821 ctttacgacg aatgaaccaa tgccaaaaga attcaagtgt acagcgaaat tccgttatcg
    17881 tcagcaagac gttcctgtga cagttcgctt attggatgat aatcatgcag aagtcgtatt
    17941 cgacgaacct gtacgtgcaa tcactcctgg acaagcagtt gtattttatg atgaaatgga
    18001 atgtctgggt ggtggtttga tcgaccacgc atatcaagaa acaaaagttt tacaatacgt
    18061 ttaagcgata ttaaatgaat caaaacagct atggctaaca aaagaaacac cttttctttt
    18121 ggatagcata gctgtttttt tgcaataaaa ttaattttta ttcattttat acttgaaata
    18181 cttgtgtaaa aagttcataa tatccaataa tatgaataaa gttgcttttt gttaagcaag
    18241 agcagttgca aaatagaagg aaatcttttg caagtgattt gttcaatttt ataagtgagg
    18301 gtgatggaaa tggaaaagaa atcaacgggt attagcaaat ggggactggt tgctctggtt
    18361 gtcagttcgt caatcggaag cggtgtgttt gggatcacaa gtgatctggc tagttctgca
    18421 gctccagggc cagcgatcct ttcatggata atcgttggga ttggtatttt agctttggta
    18481 ttatcgctga atcatttagg agaaaaacgt ccagatctag atggtggtat tttcggttac
    18541 gcggaagctt catttggtaa attaggtggc ttcatcagtg gttggggtta ctggttgtct
    18601 gcgtggctgg gaaatgttgc ttttgcaacg atgctgatga gcgccatggg agaatttttc
    18661 gcgatattca aaggtggaca aaatgttcct tctattttat ttgcaagtat ttttatttgg
    18721 ggtctgactt tattagtaaa caatggagtt gaaggagcga gcgtcatcaa tacaatcgtg
    18781 acgatctgta aattagtgcc attattccta tttttaatat ttgtatttat cgcatttaaa
    18841 gtgaatatat tcactgctga tttttggggt aacgtatctg ataatctagt gaatggaaca
    18901 gggcagcaag gaacgatctg gttgcagatt aaaggttgtt tgatggttat gatgtgggtc
    18961 tttgtaggga tcgaaggcgc aagtgtttta gccaaccgcg cgaagaaacg ttcagaagcc
    19021 cagcaagcat cgatcattgg tctgctttgt ttattgttta tctatatcct tgcttcgatc
    19081 ttgccatacg gcgtcttatc tcaagaagag ttagcaacta tttcacaacc tgctatggca
    19141 aatattttaa aaggaatcgt gggaaattgg ggagcagcgc tgatcaatat cggcttgatt
    19201 atttcgatca ttggttcttg gttgtcttgg acgatgctcc cagcagaaac aacgatgttg
    19261 atggctcgag acggtctgtt gccgaaaggt tggggaaaaa cgaataaaaa aaatgcccca
    19321 acttattcgt tagttgttac tgcaatcttg actaatctat tcttattgac tttcttagtt
    19381 actgattatg cctatcaatt tgcctattct ttatgtacag cggcaatttt gatttgttat
    19441 ctacttgtcg ggatctatca agtccagttt tctttaaaag aaggaatcaa aaaacaagta
    19501 gtgatcggtg ggattgctgt agcttttgaa ttgatgggca tcattttagc aggattttct
    19561 tatgtccttt tatgtagtat tgcctatctt cctggttttt gcttctactg gatcgcttgt
    19621 cgtgaaacca aacatcaaat tacgggaaaa gaaaaagtaa tgattgggct gatttgtgtg
    19681 gccgcactgc tttcaatcgg gttgcttgct gtaggctgga tcaagatata agggggaaag
    19741 aaacatgaaa atcgcaaatt tcggtgtaga agaatggcta aataaatggg aaaagaaagc
    19801 ggtatatgat attgcacaaa gctcgatcga agcattgaca ttagacgaac tcgttggctt
    19861 agatgggact agcgtaagtg aatttttcga aaaacgcaaa aacgaaccat tggactatgg
    19921 ctggatcgaa ggatcagatg ttttcaaaca agaagtcgct tctctttatc aaaccgtcga
    19981 tccggaaaac atcttacaaa cgaatggagc gactggtgct aatcttttag ctctatacgc
    20041 gttagtggaa gcaggagacc atgttgtttc gatgcttcct acttatcagc aattatatga
    20101 catccccaaa tctttaggtg ccgctgttga ctttgttcat ttgaaggaag aagaagattg
    20161 gcagtttgat ttagaacagc tagaagcctt agtcaaacct gaaacaaaaa tcatctgttt
    20221 gaatagtgca aacaatccta caggcacatt gctagatcgg aaaactttag aaaaaatcgc
    20281 agaaattgct cgtacagtag atgcatatgt attgatagat gaagtatatg cgcctttaac
    20341 ggataaaggt gaattcttgt cgatcgtaga tgtctatgaa aaaggtatag caaccaattc
    20401 cctatccaaa acctactcca ttccaggtat tcgtattggc tggacagcta ctggacctga
    20461 gttggcagaa gtttttcgaa agtatcgaga ctacacgatg atttgcggcg gagtcctgtc
    20521 agatgatctt gctgtacatg cattgaagaa taaagaaaaa atattggagc gaaacaaaaa
    20581 gatcattaca gagaaccttg tgattttaaa acaatgggta gccaatgaac cgaaagttga
    20641 acttgtcgct cccaattacg tctctacttc tttcattaaa ttagccatcg aagaagatga
    20701 ccagacattt tgtatcaatt tattagaaga aacaggagta ctattagtac caggaagtgc
    20761 ctttgattta ccgaagcatg cccgactagg ttactgttgt aaaaaagaaa ccttggaaaa
    20821 gggattattc ctcttatctg agttcttgaa aaaacaggcc taaacaaaaa cactgcaagg
    20881 acagtctgtg acaactgttc aagcagtgtt tttcatttac gcttattgtc tatgagaagg
    20941 agagtgccct gaagatatgg agactcctga agctttttct tctgcagttt cttgctgatg
    21001 gatctcttgt gtattgtgtc gtacaagcaa gaagttgacc aaaacaagaa gagaagtcga
    21061 aagaaagacg ccacgataac caaatgcact agaaacactg gagccgatca taggtcctac
    21121 tacgttacca gttgcttgga aagattggtt ataactgaag atccgtccag cagcttgatg
    21181 aggagaatat tttgtgatca atgcttggac agcagggagc agacaagcat cagagatacc
    21241 catcaaaaaa cgtaaggcag ctaattgcca aacattcgtg acgaatgcca ttgggatata
    21301 aaccagaatc gcaaaaccca aaccaatcgc aagtattctt tcactcccaa tcttgtcacc
    21361 taagcgacca aatcttggag cggctatcaa agtggcaatc ccaggtatcg aagcgatgat
    21421 tccgctgatc aacgtaacat tcccatgtcc atgcagcaat tggcgaatgt acagactgat
    21481 gattggacta atggaattgt tggatgcttg gatgatcatc gttgtgacga acataccgat
    21541 gacgacgtgt ggatatttca agtctttgaa aatctgtttg gcagaaacca tgttttcttt
    21601 tttcactggg acaaattttt catggacgaa aaacagactc aacaagaaga cgaaaaataa
    21661 gatgatccct gtaatgaaaa aggtagggcg atagccaaac gctgaagcag agatcccgcc
    21721 aagaagcggt ccaagtaatg ttcctgtaac agaaccagtt gctaaagttc caagaacttg
    21781 accacttttt tcttttggcg tcccagttgc gacaagggca gcggcattac tgatataacc
    21841 agaaaaaatc ccttgaagca gacgcaatgc gactaattga tacacactag tcacgcaacc
    21901 catcaaagaa atgacgattg ccattcctaa tgaagcacgc aacagcatca attttcttcc
    21961 tttttggtgg gctaattttc cccaccaagg agagatcagc gtagtcacca agaaagtaga
    22021 tgaaaaagtc aatccgctcc aaaagttcaa ttgtgacgta ctataattac ctaacgtatc
    22081 tatgtaaagt gacataaaag gcatgactaa actaaatccg ataccagcca taaatgttcc
    22141 aaaccataag acaaacaagt tttgttccca agtttctctt ttcctaaata atttttcctt
    22201 tattcttgca gccaattaga ctccttcttt ctgaaaattt tcatctacag ctttttgtta
    22261 taaactacaa tatgtagttt atcaatgaaa gtctcttttt tctctttctt tttcgtggta
    22321 aactgaaaga gtgaaggatt tattttatga gaggaaacat gaaaatgaaa aaaagaaatc
    22381 tgtctcaaga gaaaatcatc gactgctttc gagaactagc agaagagatg gatgttcagc
    22441 aaataacctt tcagcattta gcaaaacgct tggatatcaa ggctccctcg ctgtataacc
    22501 atttcaaaaa tattcgtgat gtcaaaacag cgctaacagc gaaacttctg caggaactta
    22561 atgaccagct gcgtcgggct cttgtgggaa aaagtggtca ggaagcactg cagatatatg
    22621 cagaaactta tcgatccttt gctttttcaa atcaagcagt gtatgagtta ctgatcagtg
    22681 tgccccacac caatgaagca gttttattag agggaatcca tgaaacgaac cagatcattt
    22741 tgcagatctt tgaggctttt caattaacga aagaagaaaa agtccaccga agcagagaat
    22801 tatgtagtat gatccacggc tatctttcct tgcgtttttt aggatacttt acaaaagaat
    22861 cgacaatcga tccagaggac agctattttt ggatgatcaa tgactttatc gctactttgc
    22921 cttaaacaat cagattcatt tatatcattt tcaagatgtt cattaaatga tccttttata
    22981 agagaaaatt cttgcaactt tcttgaaaag tgactaagat aagtctagaa aagctgaaag
    23041 aaggagcacg agtatgtatc aagtaattac catgttcggg gacaatgagc cttggtggtt
    23101 ttttgaagac tgggaagaag atattgaagc agaagaaaca ttcgagtctt tcgaagaagc
    23161 acgacaagcg tacgaaaaac aatggaacga aatccatcaa aattatgaat atatcaatgc
    23221 aaaaagtaac tttctcagcg ccttttggaa tgacggagat gagcgctggt gtgaagagtg
    23281 tgacgatgat ttacagcaat acaaaggact tgcgttgttg aaaaatcatc agcccatcac
    23341 agtagaaagc ggaaaggaat tttatgaaac aactaattct agcggaaaaa ccaagcgttg
    23401 cgaaagacct aagcaaggtg cttggtgcta atcaaaagca caaaaattat tatgaaggac
    23461 ccaaggtgat cgttacttgg gcccttggtc atttattagg attgaaaatg ccggaagatc
    23521 tgaataagga atggcagagc tggcaaatgg acacgttgcc aatgatccct aaaaaactag
    23581 gaatcaaacc acttcctaaa acaggacatc aattaaaagc aatcaagcag ctggctcagc
    23641 gaaaagatgt ctcagaagca gtcattgcga cagatgccgg aagagaaggg gaacttgttg
    23701 ctcgctggat cttagagtat gtgcatttca ataaaccagt gaagcggtta tggatctctt
    23761 ctcaaacaga taaagcaatc aaaactggat tcaagcaact aagacctgcg aaagaatatg
    23821 atgcattgta tgaatctgct cttgcccggg caaaagcgga ctggctggta ggattgaatg
    23881 tgaccagagc cttaactgtg aagtacgcgg ataatttatc agcaggccgt gtgcagacac
    23941 cgacactagc attagtgaga gaccaagaaa agaaaatcga gcagttccgt ccacaaacgt
    24001 atttcacgat cacgttgcaa gctggaaaag aacaagccaa aatgcttcaa aaaaatcaat
    24061 ttgctttaaa aacacaagaa gaagcacaaa aacttgttca attgatcagt acaagcaaag
    24121 gaactgtcac agaagtccaa gaaaaattaa aaacggaatc tgcgccattg ccttatgatt
    24181 taacagagat ccaaagagaa gccaatcaac gctatggcta ttctgcaaag aaaacattaa
    24241 gtcttgtaca aagtttgtat gagacccata agatcgttac ttatccgcgt acagacagca
    24301 aatatctgac aaatgacatg aaaagtacga tgaaagaacg attgcaagca gtcagcgact
    24361 ttgctcctga ggtcaagcag tatttgaaaa acggagccat cgtccaacag acaaaagtat
    24421 tccaagacag taaagtgacc gatcaccatg cacttttgcc aactgaaaac agagcacgtt
    24481 atgaaaaatt aagtaatgaa gagcaaaaaa tctatcaaat gattgtctct cgttttttag
    24541 gactgtttgc tcaaccccat aaagtcagcc aaacaaaagt tacggttgaa tttggtaaag
    24601 aacgattcat ttttcggcaa aatcgtgtcg tccaagcagg atggaaaggc gaaactgaaa
    24661 ctgagacgga aacagtcaaa tgggaaaaag aaatgagaat caatcccgat ttcaccatca
    24721 aaaaagaact atctgcaccg cctaaaccat tgtcagaagc aagcttgctt ggtcaaatgg
    24781 aaaaatacag tctgggaacc ccagctacgc gtgcagagat tatcgagaaa ctaatcaaat
    24841 ctgaactgat ggaacgtacc cctcaaggac tgcaagtatc tcctaaaggt aagcaattat
    24901 tagtattggt aaatccctct ttagtaacac cagatttgac agaaagctgg gaaaaagacc
    24961 ttgaagctat tgctgcagga aagaaacaag caggaacctt cttgaatgga atcgaaaaag
    25021 aaacaaaacg cttggtgaat gagatcaaat caagcaaaca agaatatcaa gatttctcga
    25081 ttacccaaaa gaaatgccca gaatgtgggg ccaatctgcg cgagaaaaac acacgtgacg
    25141 gcaaaatcta tgtctgcacc aattcagatt gcaactaccg cagaagaaaa gatccaaaag
    25201 tctctaatca tcgatgcccg caatgtcacc ggaaaatgga aatcatcgaa ggtaaaaacg
    25261 gtgcttattt ccgttgtaaa tacgatggaa cgacagaaaa aatgcttgat aaaaaagagc
    25321 agaagaagaa aatgaccaaa catgaagaac gacgcttgct gaaaaaatat gcacaagcag
    25381 aagaaccaga agaaagccca ttagcagcag cactaaaagc agcaatgaag aagtaataag
    25441 atatgaaaga ggttgtgata gaagtggtca gcttcaagaa ataagaagga atttacgaaa
    25501 attgcttctc aaatttttgt gaaatttcgg cttatttccg aagaagctac ttctggaaca
    25561 ccatttattc ggaaaaaagg actgtgacaa gacttttgtc acagtccttt ttcatttaaa
    25621 aaaatgttta cttcatttga cgtatattat tggtgaggct tcaattcatt tggcttcact
    25681 ttttctgttt ctcttatgat ataaacagga cgctgtttcg tttctaagaa gattttcccg
    25741 atatatttcc cgatgattcc aagacataac aattggattc caccaatgaa caggaagatc
    25801 gagaccatgg aaggccatcc tgatgtagga tcaccaaaca acaatgcccg caaaataatg
    25861 aacaaaattg caataccaga accgacacaa gacaaagcgc cgataaatga agcaatcgtt
    25921 aacggtgcat ccgagaagtt cacgatacca tcaatcgagt agttgaataa tttccagaag
    25981 gaccaagatg tttcgccagc cacacgttcg cggttttcat aagaaatata ttctgtttta
    26041 aatccgaccc agctaaataa tccttttgaa aatcggttgt attctgttaa ttctaggacg
    26101 gcatccacca tttgacgagt catcaagcgg aaatcacgag cgccatcgac catttctgta
    26161 tcgctgattt tattgatcaa ctggtagaat tttttcgcaa agaaactgcg tatcgcaggc
    26221 tcgccttcac gagtcgaacg ccgggtgccg acactatcga catcagaagt agtgatccgg
    26281 ttgatcatct caggaagcag ttcgggcgga tcttgcagat caacatccat cacggtcact
    26341 aaatctcctg tagaagcttg caatccagcg tacagagccg cttctttccc aaaatttctg
    26401 gaaaatgaga caaagcggac ataaggataa gctgaggcta gcttcttgat tgctgctaac
    26461 gtaccgtctt ttgatccgtc gtcaataaac aaatattcga actcatgggc acctttgaat
    26521 ttttcaacct catcaaaaaa aagaggaaca gttttttctt cattaaaaca tgggacaaca
    26581 attgatactt tcatcgtttc tatcctttca atcgtatgtt tatatatggt ttacattcaa
    26641 aatttaataa accttaaata acacctttta aatttgattt tatggaaaag aacagctatt
    26701 ctttggtaaa ctaacgtatt tagtttacca tgtttcctcg gtttatcaaa cttttaagaa
    26761 caatttttat tagttggcaa gtagttttgg atagagaaaa aggaggaagt tccagtctac
    26821 tgaccaggag caaacaaaat gaaaatacac acacctatcg gctttttatg gttagatgca
    26881 gatgaatccg gattgactgc tgtatctttt cagccaattt cagaaagcga atcagcgaat
    26941 cagcaaattt taacgcgcac acaagaacag tttgaagaat attttgcggg aaaaagaaaa
    27001 gaatttgatg ttcctttatc tataaagaga ggtacgcctt ttcagcaaaa tgtctggaaa
    27061 gctttgtcga ccattcctta tggagaaatc cgaacgtata aagaagtagc tgttgctgtt
    27121 gatagtccga aagctgtgcg agcgattggg caggcaaatc gaaccaatcc attgccaatc
    27181 gttattcctt gtcatcgagt gatcgggcaa aatggaaaac tgacaggata tatgggaaac
    27241 gcaatagatg gggttgaaat caaacgccaa ttgttgttgt tagaaggata tctgcaaaat
    27301 taaaaacagt tgtgattcgg acattgttcc atgcgagaat cacaactgtt ttttcatgtt
    27361 cattctaaac tttgatcagc caaataaata gtaaagccat cactgtttgg agaagcccga
    27421 cagctaatcc ataagataag aagcgtgtac cttctttcat gaattttgca aagtctaaac
    27481 gcagaccaat tgccgctaaa gcagtgatct caaaccagct gctgataaaa tgtgccgatt
    27541 ggtcaataac tacaggtaaa gaaaacaagc tgttgaccaa acaaaagatc acaaacccta
    27601 atacatacca aggaagtggt aattttcgtt ttttctgcgt atcagttggc gtttcaaaca
    27661 gagctggttg tttcttgtgc atgaattttt caaacagata gacaacaaca acgagcatca
    27721 taatacgtag aatcttaaat agcatggcga attcgacaac agaattgtca agtaagcttg
    27781 ccccagcgac tacttgacca acagattgga gtgtgccacc caaaagtgca cttttttcaa
    27841 tgagatttcc accaaacaac tgtaatccaa ggaaagggag agtcaacatc atcacagtac
    27901 ccagtagatt gactagtgtg atgatctgtc ctttttcttc ctctttcgca tggatggaag
    27961 gggcgatcga agcaatagca gaagaaccac aaacagcgtt tcctccagcc atcatcaaag
    28021 aagaagtatc gctgaattgc agttttttac caatcaagta agcaccgatg atagtgactg
    28081 acatttgcag taacacaaaa acaattcctt ttataccaat ttgagcaatc gtttgagctg
    28141 tgacagttgc acctagtaaa aaaacagaga attctaatag tttgctttca gcaaattttg
    28201 tgccgattgc tagaacaggc tgtttaaaaa aagtatttcc taataaaata cccaataaaa
    28261 tagcaatagt cgctccacct aactgtggca gataaatagc aagaagcctg ctgaaggcag
    28321 cgatggcaaa agaaagcagt aaaccgggta agatttcgcg gaccgctgaa tcctttacag
    28381 attttattgg atgtgacaat gatttcattt gatcggttcc tttcgtcaat aaatactaaa
    28441 agcatactcg aacatagtag cactaaacca gtattttgac aaaagaacaa gaggtcgtga
    28501 caaaagtctt gtcacgacct cttgtttcg
//
LOCUS       DACGLE010000031        28300 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0534
            SAMEA3304449-rid9560453.denovo.032, whole genome shotgun sequence.
ACCESSION   DACGLE010000031 DACGLE010000000
VERSION     DACGLE010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304449
            Sequence Read Archive: ERR985064
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 28300)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28300)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702225.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702225.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 20:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,045
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 27 of 67
            Pseudo Genes (incomplete)         :: 25 of 67
            Pseudo Genes (internal stop)      :: 19 of 67
            Pseudo Genes (multiple problems)  :: 4 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28300
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304449-rid9560453.denovo.032"
                     /strain="VREN0534"
                     /db_xref="taxon:1352"
     gene            complement(3..1343)
                     /locus_tag="IXT20_001458"
     CDS             complement(3..1343)
                     /locus_tag="IXT20_001458"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sapep family Mn(2+)-dependent dipeptidase"
                     /protein_id="HAQ4859912.1"
                     /translation="MKKFVTEQHHEQALESLSELIRIPSVLDEADSGQGHPFGKKVIE
                     ALDKVLEISEKLGFRTFKDPEGYYGYSEIGSGDELFGILCHMDVVPAGDENNWETKPF
                     DPTIKDGWLVGRGSQDDKGPSIAAMYAVKALIDAGVEFKTRIRFIFGTDEENLWRCLE
                     KYNEKEEGITQGFAPDAEFPLIYAEKGLLQAYLTGPGTNEFSVKAGGALNVVPDAAPY
                     SGEKLSEVKEALKKHEFDFEEQGEGIVVLGKSIHAKDAAQGVNAISRLAIALSEVFDF
                     GPINFLGKLVQENATGEAVVGKTEDEQSGELTMNFASLEITPEQTKIGVDMRIPVTFK
                     KDDLVAKLTKTAEKYGLTYEEFDFLDSLYVPLDSELVKNLLGTYRDITGDMTEPFVSG
                     GATFARTMNQCVAFGAMFPDTPDFMHQANERWELSSMYKAMEIYAEAVYRLCAK"
     gene            complement(1580..3103)
                     /locus_tag="IXT20_001459"
     CDS             complement(1580..3103)
                     /locus_tag="IXT20_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HAQ4859913.1"
                     /translation="MEQTKKKKFQMPSAYTVLFIIIAVIAVLTWFIPAGHYSVDDAGN
                     IVAGSYERVQQNPQGLWDIFMAPINGMLGVPAGELTKETPAAIPISFFILVVGGFLGI
                     INKTGALDAGIASVVKKFKGKEKMLIPVLMLLFALGGSTYGMAEETIPFYALLIPVMM
                     AVGFDTVVAVAIVLVGSQVGCLASTVNPFATGVASQAVGISMGEGIGLRFLMFVILLA
                     ISIVYVYRYASKIEKDPTKSLVYNQRKEDMKHFDIEAIGRQEVITKKQKHVNVLFFIT
                     FGIMIISLIPWTTLNANFTIFESLTNWLTNLPVLGTVLGKSMLPLGDWYFPEITMLFL
                     VMSIVVAITYGMKESEFVSEFLAGASDLIGVAIVVAVARGIQVVMNNGLITDTILHWG
                     EQGLSSLSSSVFIIITFIFYIPMSFLIPSTSGLAAATMGIMGPMGEFAGVGKDLVITA
                     YQSASGLVNLITPTSAIVMGAVAIARFDVSVWWKFTGKLIAILFVAICVILGVAAMF"
     gene            complement(3481..4092)
                     /locus_tag="IXT20_001460"
     CDS             complement(3481..4092)
                     /locus_tag="IXT20_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAQ4859914.1"
                     /translation="MKNTRVQKMVAVAMFAAIALVLQYFAFPIMPAFSFLKIDFSDIS
                     VLISMFLFGPAAGVATAFLRSVLHLLTTGFSPDNLVGDTASFLSTTIFTFPIYYFFSV
                     KKQHKGRNKFLGVITGTIALTIFMSIANYFVITPLYLMFLGLNATQMLGMSLANYVLI
                     GIVPFNLIKGFIVSAVFLVLHAQLLPWLSRKQHQFEQRHTAVK"
     regulatory      complement(4215..4340)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            complement(4422..5138)
                     /locus_tag="IXT20_001461"
     CDS             complement(4422..5138)
                     /locus_tag="IXT20_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HAQ4859915.1"
                     /translation="MERLQKVIAHAGITSRRKAEEFILAGRVKVNGQVVRELGIKVGK
                     HDTVEVDEVPIYQEEYGYYLLYKPKGVISAVADDKGRKVVTDLLPMVKERIYPVGRLD
                     YDTSGILLLTNDGDFAQRLTHPKHEVDKVYVAKVKGIATKSMLAPLTKGIKIEGRKTS
                     PAKFQILSVDPKTNHSIVELTIHEGRNHQVKNMLQAVGLPVQKLKREKYGDLTLQGLR
                     PGDYRELNKKEVSSLLNHSK"
     gene            complement(5144..5713)
                     /gene="scpB"
                     /locus_tag="IXT20_001462"
     CDS             complement(5144..5713)
                     /gene="scpB"
                     /locus_tag="IXT20_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HAQ4859916.1"
                     /translation="MTKLSELEALLFIVGEEGISNEELSGLLNLSEAEVTSLVKELKE
                     SYTQNSHSALHIFETESHYVLTTKKELAPLLKRYAQGTSNTLSQAAVETLAIIAYKQP
                     ITRAEIEQIRGVQVSGAVQRLAAHKLIEEKGRVEGPGRPILYGTTGYFLDYFGLKSLE
                     ELPDTQEMEISLEEDVSPDLFFDGMNKGE"
     gene            complement(5697..6494)
                     /locus_tag="IXT20_001463"
     CDS             complement(5697..6494)
                     /locus_tag="IXT20_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HAQ4859917.1"
                     /translation="MASKEKEERVNELTEINIKLDVFEGPLDLLLHLIQQMEIDIYDI
                     PIATITEQYMNYIHTMKTLELAVAGEYLVMAATLMAIKSKTLLPVQEFVTDDEELWEE
                     DPREELVTQLLEYRKYKYAAERLTEKAEERSLYYTKEPMNIDGLADAEKPLKRGQVKK
                     IDLIRAMQKILERKKFSQKVEKTITPDDTTIEERMVFIEERIKRNAHGCTFDSFFESP
                     SKSEVVTTFMALLELMKKGQIIAQQKENYESIMLYPAVGDTEHDEIK"
     gene            complement(6464..6835)
                     /locus_tag="IXT20_001464"
     CDS             complement(6464..6835)
                     /locus_tag="IXT20_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HAQ4859918.1"
                     /translation="MLTHYRKENRKIAMGLLSFHKKLTGKTSLLKEIDTYESEDDYEL
                     FFFTPEGSTNIQGIIGIYWATNDQLIIHDISLNPSYRGENMGFQMLDELQEKYPDVSI
                     RATEITEPYIGKWLAKKKKKE"
     gene            complement(6854..7741)
                     /gene="xerD"
                     /locus_tag="IXT20_001465"
     CDS             complement(6854..7741)
                     /gene="xerD"
                     /locus_tag="IXT20_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HAQ4859919.1"
                     /translation="MKEQINDYLHYLNIERGLSANTRQSYERDLEQYLHFLQEQKIAS
                     WQDVDRFLVIRFLEKLHEEKKATATVTRMISSLRRFHQFLRQERITDHDPMQHIDTPK
                     KAQKLPSTLTLTEVEKLIEAPDTTKTLGIRDRAILEVMYATGMRVSELIGLKLGDLHL
                     SLGLVQTVGKGDKERIIPLGDYAIQWLERYLDEARPLLVKDASEMHVFVNNHGKGLSR
                     QGIWKNLKQLVREAGIYKEVTPHTLRHSFATHLLENGADLRTVQELLGHADISTTQIY
                     THITKKRMTDVYKQHFPRA"
     gene            complement(7837..8310)
                     /locus_tag="IXT20_001466"
     CDS             complement(7837..8310)
                     /locus_tag="IXT20_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HAQ4859920.1"
                     /translation="MVMNAISAMKKTKQQLHEAGFKLTPQREATLLVLLENEKEHLSA
                     EEIFFLVKKKSPEIGLATVYRTLEILTELKIVDKVSFNDGLARYDLRKEGAKHFHHHL
                     LCLNCGTIEEVEEDLLLEVEQVVEERYHFLVKDHRLTFHGICQECYEKEKRNQSV"
     gene            complement(8415..9278)
                     /locus_tag="IXT20_001467"
     CDS             complement(8415..9278)
                     /locus_tag="IXT20_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HAQ4859921.1"
                     /translation="MNDLLATIFTGIIIDENEKNYFVQKNGQTFGLSKSEGEHKIGEA
                     VEGFGYQNQKHENRITTQIPKSRIGHYAFGIVTATRKDLGAFVDIGLPDKDFVVSLDE
                     LPTMTELWPKKGDRLMISLRVDAKDRIWGSLADEKIFKSLSKYGTEEMKNKDVIGTAY
                     RLKLTGTYVLTEDFYLGFIHPSERYNEPRLGEQIKGRVIGVRPDGVLNLSLKPRAYEA
                     ISDDAQMILTILERSADQQINFTDKSDPEEIMKAFGISKGAFKRALGNLLKQGLITQE
                     NGVTKLVKKSN"
     gene            complement(9474..11573)
                     /locus_tag="IXT20_001468"
     CDS             complement(9474..11573)
                     /locus_tag="IXT20_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HAQ4859922.1"
                     /translation="MKKCPNCGSKKMEQNTICPDCGHQITAEDQQIKESIEETNTDAA
                     MQDKSTEDAVFPDTELNDPIEWSELKDLPLESVMELFEKAEPEDEEPPAHDQVTNIEK
                     NEQKKATVHETSEESGKNQEETLESKQKRRVAELKEIVDNEEENSILSAYIKAHREDT
                     KEEHAKELLKMISEKIAQENKVEPEKEQETEKDSDRENATGKETVKIKDVKTDITEKT
                     EVFTEKQLEKEKTNKLPEAEKKEILENTDKKSTEPDEINSESDAENQSVSKEGTDLEA
                     KIEKEENQPKLFEAEAKSITIVPDTEKTHQESNLEKTDPDKAQKKKSKKVPYLVLAAV
                     LLLGGGSWAYYDHHQKVEAEVAAEAKRQQQMEELHEKLAAFYTDDHHQFIRADMIHQD
                     LSKLKNDLSSLKNEKGYSELETTYQDIQSKIRDIQKVNEWFIAPAIVDDHLADDIKLK
                     ADKEIQAIETDNTDFGKLIQQAQTEAKNQYAELQTAKEKTAIVFKDGKVVDSATRDQY
                     KTAKEAVEKVKNTDLSAQLNEQLKQVDKMLTDKEKKEEEEKKKAEEAKKAAEEQAKQA
                     AQAAAAAQAAQQAAVAQAAQQEAASSTPSSSNSANQPIKSARASDIADASNPAWTWAP
                     GVQSKVIATCIQRGYIVEGGYRLEKARIENGEGYYNLYATSTKSALMKGIGESALPFY
                     IVTINCKTGWFGGNGSN"
     gene            complement(11793..12902)
                     /gene="rpoD"
                     /locus_tag="IXT20_001469"
     CDS             complement(11793..12902)
                     /gene="rpoD"
                     /locus_tag="IXT20_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HAQ4859923.1"
                     /translation="MANGTDKKTYEAAVAAFIKENKPKRQVVYDDLSNQLATPFTLNA
                     DEMDKLIQKIEDAGISVVDENGDPSIHSLKSAEKKAEQAKTEDLSAPTGVKINDPVRM
                     YLKEIGRVSLLTAEEEVALALKIEEGDQEAKQRLAEANLRLVVSIAKRYVGRGMQFLD
                     LIQEGNMGLMKAVEKFDYRKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETIN
                     KLIRIQRQLLQDLGREPTPEEIGAEMDLPTEKVREILKIAQEPVSLETPIGEEDDSHL
                     GDFIEDQEATSPAEHAAYELLKEQLEDVLDTLTDREENVLRLRFGLDDGRTRTLEEVG
                     KVFGVTRERIRQIEAKALRKLRHPSRSKQLKDFLE"
     gene            complement(12926..14767)
                     /locus_tag="IXT20_001470"
     CDS             complement(12926..14767)
                     /locus_tag="IXT20_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HAQ4859924.1"
                     /translation="MAQRIPQEVIETVRSQTNIVEVIGQYVQLKKSGKNYLGLCPFHE
                     ERTPSFSVAEDKQIFHCFGCGKGGNVFTFLQELEGLSFPEAVVKVADFEQIPLEDQWR
                     QRTIAVQNPESATGKLIAAHEKAAEIYHHMLLHTKAGQPALEYLQSRGLSLELIEEFN
                     IGFAPNERSFLQQVFKNESISNELLERSGLFVTHDDGRMSDRFYQRVMFPLRNSQGQT
                     IGFSGRWLAPEKGQEKDQPKYLNSPETELFNKRQVLFNLDKARSDIRKNGEILLFEGF
                     MDVIAAWQAGIKTGLASMGTSLTAQQIQQMEKLTKELVFCYDGDNAGFEATNRGIELL
                     RQNSRLALSVVVVPEKLDPDEYLRKYGEASFQELVAHGRETVFTFKSRYLKQGKNLDN
                     EKDKIEYLEELLVELSRVVSPIEQDMYLSQLSTEFQIGRETIQKQLRDLRRTNQQNRQ
                     EPQESQHTQVTRQETARKKRPLTQVEKAEQILLYRAFKEVSVRNILKEREFQFIHDAY
                     AEVYFLFDAYVSQHYEFNLAEFLDFLQDENLRRLVVEIEYLPVAEESSPREIQDLLDV
                     ISKSGLADEITLKKQMQQEARRTGNKQLELELTIEIINLTKQLKQAK"
     gene            complement(14949..17342)
                     /locus_tag="IXT20_001471"
     CDS             complement(14949..17342)
                     /locus_tag="IXT20_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HAQ4859925.1"
                     /translation="MFLILEHARLKHVIIFESWRKMEKKKMNGLTQQEVVQKMASGQQ
                     NDYQEDASKSTKQIFSDNILTLFNFLNFGIGICLLFVGAYSNMAYLAIIVVNIVIGIY
                     QEIHARNLVRKLSIVSQSEVLVIRDGKEQSISTKELVLGDLVKIGAGEQIPADLQVIS
                     GRAEANEALLTGESDLIVKETGAELLSGSFLTSGSVMAEVIRVGADNYAVRLTSEAKT
                     HKAIRSELVDSIRKVSKFTSFVIIPLGIILFLEALFIRDASIKISVIASAAALLGMLP
                     KGLVLLISIALTTGVTKLAKKRILVQDMYSIETLAHVDTLCLDKTGTITEGKMSIQRV
                     EALHPLYEKAIPEIMGTYLAESSDNNITMRAMRDYFELSNRYDVTNKVAFSSDRKWGA
                     MEFPELGVVYVGAPERLVEEDRLPDSLKTAQANGYRVLMLGIAPNQQLNEEKPAFVEP
                     VALFEIDDPIRKNAQETLAYLHEEGVDLKVISGDNPFTVSNIARRAGLPNYDAYVDLS
                     QITEEMEVREAAHRYTVFGRVSPQQKKLLVNELKESGRTVAMTGDGVNDVLALREADC
                     SIAMAEGDGATRQIANLVLLDSDFTTLPEVLFEGRRVVNNVTKVSGIFFIKTIYSFIL
                     SIICAVTAIGFPFIPIQITLIDLAIEGYPSFFLSFEQDKRKITYRYLPTALINALPNA
                     LLVVVNIIAVYLLGKTFAWGNAETTTLMYYLLIGISCMAVVKACYPFNPLRIFLAVTT
                     IVGIYVAAMLFHGILEVNLGLGESVGYFFVFMLVNIVLRVLYWKADIQNQLLKRVAN"
     gene            complement(17449..18309)
                     /locus_tag="IXT20_001472"
     CDS             complement(17449..18309)
                     /locus_tag="IXT20_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HAQ4859926.1"
                     /translation="MEKRQKMENQEVGYTTKDWMLRFVKGMFIGSGFILPGVSGGALA
                     AVFGMYERLISFLAHLTKNFKENVLFFLPVGLGGVTGIFLLSFLVSFLLGAYETTILW
                     FFVGCIAGTVPALWKEAGKKGRDTSDVIIMVVTFVLAYLFLLYGAQLFDGQVDQNFGT
                     WLIAGGLIGLGMIVPGLSPSNFLVYMGLYKAMADGFKEVRLEVIVPIGIGGILCVLGL
                     SKIMDYIFSKAYSKLFHFILGVVFASTIMIIPTDYAGLGVAGVLACPVLFIAGTALGW
                     WMSRLEEQYK"
     gene            complement(18368..19000)
                     /locus_tag="IXT20_001473"
                     /pseudo
     CDS             complement(18368..19000)
                     /locus_tag="IXT20_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289460.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme family protein"
     gene            19209..19928
                     /locus_tag="IXT20_001474"
     CDS             19209..19928
                     /locus_tag="IXT20_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAQ4859927.1"
                     /translation="MKKEKKYAKMPDGSEIYYEKSGQGFPLFLLHGNDGSGRFFSEQV
                     PVLERYYTVYLVDSRGHGRSTNEASMLNFQLMAEDLNTIMLLEKIDQADFLGFSDGAN
                     LALVFASSFPKKVHRLILNSGNTLVKGVRFSARVISNIHYAWVWLLSLFRPSLRKNLL
                     VIKLLLHDIGLTENDLKKINSPTLIIVGKKDVIKLKHSLYIAKTIPKASFVLVKEQGH
                     ELARKDPERFNREVLQFLSET"
     gene            19954..22545
                     /gene="mprF"
                     /locus_tag="IXT20_001475"
     CDS             19954..22545
                     /gene="mprF"
                     /locus_tag="IXT20_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HAQ4859928.1"
                     /translation="MLKNTIQWLKNHLGLFKTIFLISVIVIIVGELMSIGKTLSIQQL
                     GETFATIPVWKSGLMLLIGLISVLPMVGYDIILNRMLEQKQNPRYLFETSWLINTINN
                     IAGFGGFVSVGLRSELYGQKKESRNVIQALSKVFLFLMAGLSIYSLLSFLLVVFTPVA
                     PFLKQYWIWLIGGGLYFPVVFLFTTFKKKGFLGGTSLKTQGQLLLVSLLEWTGVLFSF
                     LSVGYLMEIRIDLWQTIPLFIAASVIGIVSMIPGEIGSFDVMMIIGLSAIGVPREIVV
                     VWILLYRLFYYIIPFLIGIVFFFKNIGSTFDQRYSGIPKQLATEIAHKIVVVLLYFSG
                     IMLVLSATIPQAFTEFRWLHSLNPLKFHFIIQFPSILLGFLLIIMGRGIAARVKRAYL
                     PTIFLIVLALLYVLLSDFSFTPVVFLSILLLIILASKNELFREQLIYSWEWRTIDGII
                     IGALSLLYIVIGVYNLPDFPHRRHHFISFFLFPSEKIWFSGLLAIIAVSFMIVLFVHF
                     LQGEKKQIGEAFNEEKALKILTTYGGNSDSQLIFLKDKRMFAYEKDGEPTVLLQFACF
                     NNKCIVMGDPSGKKEDFPEAIEAFIEETDRLCYLPVFYETSEEIVMILHEFGYDFIKM
                     GEEAYVDLNSFTTSGKKMKGTRAVLNRIEREGFTFDVLQPPFSTEQMSTFKNISDNWL
                     GSRKEKGFSLGFFSEDYLQRAPIAVVKNTQEEIVAFATIMPTYTNNKVGTIDLMRYDS
                     KKAPSGSMDFLFLHLFAYMKEEHIQWFNLGMAPLSNVGTSRKSFLQERIAYLVYEFGS
                     HFYSFHGLREYKSKYATNWVSRYTLYSRDSWIAYVMIALLIIDNAPVEQTQGRISGLK
                     KWIRRKY"
     gene            complement(22733..23920)
                     /gene="tuf"
                     /locus_tag="IXT20_001476"
     CDS             complement(22733..23920)
                     /gene="tuf"
                     /locus_tag="IXT20_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HAQ4859929.1"
                     /translation="MAKEHYDRSKPHVNIGTIGHVDHGKTTLTAAITTVLGKKGLANP
                     QDYASIDAAPEERERGITINTAHVEYETEKRHYAHIDAPGHADYVKNMITGAAQMDGA
                     ILVVSATDGPMPQTREHILLSRQVGVKYLIVFLNKVDLVDDEELIDLVEMEVRELLSE
                     YGFPGDDTPVIKGSALKALQGDPDAEAAIMELMDTVDEYIPTPERDTDKPLLLPVEDV
                     FSITGRGTVASGRIDRGAVRVGDEVEIVGIKPETQKAVVTGVEMFRKTLDYGEAGDNV
                     GVLLRGIQRDDIERGQVLAKPGSITPHTKFKAEVYVLTKEEGGRHTPFFNNYRPQFYF
                     RTTDVTGTITLPEDTEMVMPGDNVTIDVDLIHPIAVENGTTFSIREGGRTVGSGIVTE
                     IEA"
     gene            24288..24644
                     /locus_tag="IXT20_001477"
     CDS             24288..24644
                     /locus_tag="IXT20_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HAQ4859930.1"
                     /translation="MKKLIGVLVVLGIFLGGSFAAYHYFYGGEAYYTKITTNGEMSAT
                     QADNGEKFTTYTYKQAAYNKDGEKKQVTLREEREHPLKMNAYLKLKVNPRKGVLSWEE
                     IKASEVPKNAAEKIDN"
     gene            complement(24885..25889)
                     /locus_tag="IXT20_001478"
     CDS             complement(24885..25889)
                     /locus_tag="IXT20_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HAQ4859931.1"
                     /translation="MDIYLKKAILHIIDRETGSPVFSQKELDLTKEYIRDFLQKKIQK
                     ISSAQTKTGQLAEESMIVESLKIAQENFVEASEAIVQRWFDIYQESEEAPSADVFVVL
                     YELDTVMYLSLLKVNYREAYTHFVEADEAGIDNRLILNRAILGAKTQKADEAFAVNLT
                     DWTYELIEKKYEFSGKKELYFSGRVIESVPAPSLEENVKVIQKVAKHLGKKFETEEFD
                     IMADLKEAVYDTIEEKGRLDHEMIAEKVFKENITAKMAFKEEVQEQGFVPEAPPVKEV
                     QEISEKKFGKQKFKLSNGIELIVPVDVYRNPDLIEFVNNPDGTISVMIKNVDEVLNRL
                     "
     gene            26055..26906
                     /locus_tag="IXT20_001479"
     CDS             26055..26906
                     /locus_tag="IXT20_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HAQ4859932.1"
                     /translation="MNSLTSTYRLSNGYEIPVVGFGTWQTPDGDVAVSSVKEALAAGY
                     RHIDTAQGYKNEESVGQAIKESGIPREEIFLTTKLWNANHSYELVMSSFEESLKKLQT
                     DYLDLFLIHWPNPVAFRDNWEQANADTWRAFEELYEAGKIKAIGVSNFLPHHLDTLAK
                     TAKIMPMVNQVFLAPGELQTTVVEYAKKHDMILEAYSPLGTGKIFDVPEMKQIAEAHD
                     KTIAQVALRWSLQHEFLPLPKSVTPSRIKENTELFDFELTEEEMKQIDQLDGVVGKAK
                     DPDTTQF"
     gene            26923..27168
                     /locus_tag="IXT20_001480"
     CDS             26923..27168
                     /locus_tag="IXT20_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4859933.1"
                     /translation="MDPIERLNSLSEEVTQTFHSDFVFLIDAEKIQHFPARNWTHDQI
                     IEELKKRFDHSLMVKPWHEHEVIYSPELPVFALIPKK"
     gene            27355..28059
                     /locus_tag="IXT20_001481"
     CDS             27355..28059
                     /locus_tag="IXT20_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4859934.1"
                     /translation="MKKKQKQKLLNQFRPSLDGVRGQLFRSLEEESLRRYGLPLQVML
                     DPKNRQILTIGLAGKTNEDTRINVPIDDNFNTVLKRIRSGENGIFERFRDNLLIEIVS
                     YWNDQRLKNDEPTAQAVATPVTEETVSVPEEKTEKPKADTTENTRSTDTNAALSFADF
                     SKAVAEYPKFYTEQNEHEVVINEKNADEPRKLATISMTTENEFVIEKALERKYKVKLT
                     LIPLIEQFAATAITDR"
BASE COUNT         8028 a         5905 c         4901 g         9466 t
ORIGIN      
        1 ttttatttag cacataaacg atacacagct tctgcataaa tttccattgc tttatacata
       61 ctacttaatt cccatcgttc attcgcctgg tgcataaagt caggagtatc agggaacatc
      121 gcaccgaatg ccacacactg gttcatcgta cgagcaaaag tagcgccgcc tgatacaaac
      181 ggttcagtca tgtctccagt aatatcgcga tatgtcccca atagattttt cactaactca
      241 ctgtctagtg gcacgtataa tgaatcaagg aagtcaaatt cttcataagt caacccatat
      301 ttttctgctg ttttagttaa tttagctact aaatcatctt tcttgaatgt aacagggatt
      361 ctcatatcta caccgatttt tgtttgttct ggtgtgattt ccaaacttgc aaagttcatc
      421 gttagctcgc cggattgttc atcttctgtt ttgcccacga cagcttcacc agttgcattt
      481 tcttgaacta attttcctaa gaaattgatt gggccaaaat caaatacttc tgaaagagca
      541 attgccagac gagaaatagc attcactcct tgtgctgcat ctttcgcgtg gatactttta
      601 ccaagaacga cgatgccttc accttgttct tcgaaatcga attcgtgttt tttcaatgct
      661 tcttttactt cagaaagctt ttctccagag taaggagctg catcaggaac aacattcaac
      721 gctcctccag cttttacaga aaactcgttc gttcctggtc ctgtcaagta agcttgcaat
      781 aagccttttt ctgcataaat taaaggaaat tcagcatctg gcgcaaatcc ttgcgtaatt
      841 ccttcttctt tttcgttata tttttctaaa caacgccata aattttcttc atctgtacca
      901 aagataaaac gaatacgtgt cttgaattcc acgccagcat caatcaaagc tttgacggca
      961 tacattgccg caatagaagg acctttgtca tcttgggatc cgcgaccaac taaccagccg
     1021 tcttttatag ttggatcgaa tggttttgtt tcccaattat tttcgtcccc tgctggaaca
     1081 acgtccatat gacacaagat accgaataat tcatctccag agccaatctc tgaataaccg
     1141 taatagcctt ctggatcttt aaatgttcta aatcctagct tttcactgat ttccaatact
     1201 ttatccaaag cttcaatcac ttttttaccg aagggatgtc cttgtcctga atctgcttca
     1261 tctagtacgg aaggaattcg gatcaattcg ctcaatgatt ctaatgcttg ttcatgatgt
     1321 tgttctgtca caaatttttt catgatatta tcctcctaat atgttaaaaa gggtcatgtc
     1381 aaattcctcc gacacaacct cttcttctca aataatgacg ttttaagcct gctccatcag
     1441 caagcagaat gttcttaaca aatgatgaaa gaaaaaacac tcactgaatg gagctattga
     1501 cttatccacg tgcttatcga tagccgttta tcggtttctt cattcttatg atagaccttg
     1561 tacctgtatt tgtctaggtt tagaacattg ctgctacacc taggatcaca cagatggcta
     1621 caaataatat cgcaattaat ttaccggtaa atttccacca cactgaaaca tcgaaacgag
     1681 caattgctac agcacccatt acgatagcag atgttggtgt aattaagttt actaaaccag
     1741 atgcagattg ataagcagtg attactaaat ccttaccgac gccggcaaat tcacccatag
     1801 gtcccatgat tcccattgtt gcagcagcta agccggaagt tgaaggaatc aagaatgaca
     1861 tagggatata gaaaataaat gtaataatga tgaatacact tgaagataat gaacttaatc
     1921 cttgttcgcc ccaatgtaag atggtgtctg tgattaatcc attgttcata accacttgga
     1981 taccacgagc aacagctact acgattgcta caccgatcaa atcagaagca ccagctaaaa
     2041 attcagagac aaattcagac tctttcatgc cataagtgat tgctactacg atggacatta
     2101 ctaagaataa catagtaatt tctgggaagt accagtcacc taatggaagc atgcttttac
     2161 ctagaaccgt acctagtacc ggaaggtttg ttaaccagtt tgtcaatgat tcaaaaatcg
     2221 tgaaattcgc attcaaggtt gtccaaggaa tcaaactgat gatcatgata ccaaaagtaa
     2281 taaagaacaa tacattaaca tgtttttgtt ttttcgtgat tacttcttga cgaccgattg
     2341 cttcaatatc gaaatgcttc atatcttctt tacgttggtt gtatactaat gattttgttg
     2401 gatctttttc gatctttgat gcataacgat acacatatac aatagaaata gctaacaaaa
     2461 taacgaacat caagaatcga agaccaatac cttcacccat cgaaatccca acagcttgtg
     2521 aagctacgcc tgttgcaaaa ggatttaccg ttgaagctag acagcctact tgagacccta
     2581 ctaatacgat tgctacagct actactgtat caaagcctac agccatcatg actgggatca
     2641 gtaaagcata gaatggaatc gtttcttctg ccattccgta agtagagccg cctaatgcaa
     2701 ataacagcat caatactgga ataagcattt tttcttttcc tttgaatttc ttcacaactg
     2761 aagcaatccc ggcatctagt gcccctgttt tgttgataat acctaaaaat ccaccaacaa
     2821 ccaaaatgaa gaaagagatt gggattgcag caggtgtttc tttagttagc tcccccgctg
     2881 gtacacctag catcccattg attggcgcca tgaaaatatc ccacagccct tgaggatttt
     2941 gttgtactcg ttcatatgat ccagcgacga tgttgcctgc atcgtctaca ctatagtgcc
     3001 cagctggtat aaaccaagta agtacagcga ttactgcaat aataataaat aagaccgtat
     3061 acgctgaagg catttgaaat tttttctttt ttgtttgttc catcttcttc ttcctttctt
     3121 ttccgttaga gacaacaatt ttggctctaa tgttatttta tcaaaaagag tatcagaaca
     3181 aaaataataa cacgcatttt gtaaaacttt tgttcaaaaa gttcgttttt ccctattccc
     3241 tcctcttttt tcaggttttt aaagctttct tctttttcat tgtagcgttt cctttttttt
     3301 ataaacaaac attttcgtct tttaaaagga gaagaatttc acatttgcaa agaattgatt
     3361 gatttttatg cataaatcaa aaaaatactc aaaacaaaca aataaaacgt ttacattatt
     3421 gcatgtcttg tatttttttc caaaaaaaaa gaccaagaaa tttcttgatc tttttgatat
     3481 ttatttgact gctgtatgac gttgttcgaa ctgatgttgt tttcgactta accatggaag
     3541 aagttgcgca tgcagcacta aaaagacagc actcacgatg aatcctttga tcaagttgaa
     3601 aggaacgatc ccaatcagta catagtttgc taaagacatg cccagcatct gtgttgcgtt
     3661 cagccctaaa aacattaagt aaagaggcgt aataacgaaa taatttgcga tactcataaa
     3721 aatagttaga gcgatggttc ctgtaatgac acctaaaaat ttgtttcttc ctttatgctg
     3781 tttctttaca ctaaagaaat aataaatcgg gaatgtaaag atcgttgttg ataagaaact
     3841 agcagtgtct cctaccaaat tatccggaga aaaacctgta gtcagcagat gtaatactga
     3901 acgcaagaac gctgttgcaa ctccggccgc tggaccgaat aagaacatgc tgatcaatac
     3961 cgagatatca ctaaaatcga tcttcaaaaa actaaaagcc ggcatgatcg gaaaagcaaa
     4021 atactgtaaa actaatgcga tcgcagcaaa catcgctact gcaaccattt tctgtacacg
     4081 ggtgttcttc atttgaaatc ctcctgttag gactcctctt cagcgaactt tttgacgttt
     4141 ctctccaaat gaaaaaactc tattttccag ggaaaataga gttagagtta tttgatcaag
     4201 cgtcgtcaaa taagctctat cttctttatc cagactatac tgtcggtatc ggaatttcac
     4261 cgattcagct atttttttgc aaaataggtc gtggactata accaccggtc gggaatttca
     4321 ccctgcccct gaagacgaac cttatataat ttttgtttac atattcatta tacatatcta
     4381 ttcaaaaaac gcaacatcta acacttataa aaagtaagtg ttcattttga gtggttcagc
     4441 aaactgctga cttctttttt gttcagttca cggtaatccc caggacgtag gccttgtaaa
     4501 gtcagatctc cgtatttttc acgttttagt ttttgcacag gaagaccaac tgcttgaagc
     4561 atatttttca cttgatgatt acgtccttcg tggatcgtca attccacgat actgtggttt
     4621 gtcttaggat ccactgataa aatttggaat ttagctggag atgtttttcg tccttcgatc
     4681 tttattcctt ttgtcaaagg tgcaagcatc gattttgtag cgatcccttt tacctttgca
     4741 acgtaaactt tatccacttc atgttttgga tgtgtcaggc gctgagcaaa atctccatca
     4801 tttgtcagta gcaagatccc cgaagtatcg taatccagtc gacctacagg atagatacgt
     4861 tcttttacca tcggcaaaag atccgtaaca acttttcttc ctttgtcatc tgcaacagca
     4921 gaaataactc ctttaggttt gtacaataaa tagtaaccgt attcttcttg ataaattggg
     4981 acttcatcca cttccacagt gtcatgtttc ccaactttta tccctaattc ccttacaact
     5041 tgtccgttta cttttacacg tcctgcaagg atgaattctt ctgcttttcg acgagaagtg
     5101 atgccagcgt gggcaattac tttctgcaat ctctccatta caattattct cctttattca
     5161 ttccatcaaa gaatagatct ggtgacacgt cttcttcaag tgaaatttcc atttcttgtg
     5221 tatcaggcag ttcttctaat gatttcaaac caaaataatc taagaagtag ccagttgttc
     5281 catataatat cggccgacct ggtccttcta ctctaccttt ttcttcaatc agtttatggg
     5341 cagctagacg ctgtaccgct cctgaaacct gaactcctcg aatctgctcg atttcagctc
     5401 ttgtgattgg ctgtttataa gcgatgattg ctaaggtttc gacggcggct tgagataatg
     5461 tgtttgatgt cccttgcgca tatcgcttaa gtaagggagc cagctctttt ttagtcgtta
     5521 atacataatg gctttccgtt tcaaaaatat gcaaagctga gtgcgagttt tgagtgtaag
     5581 attcttttaa ttctttcact aaacttgtga cttctgcttc gctgagattt aacagtccgc
     5641 ttaattcttc attactgatc ccttcctcgc caacaataaa aagaagggct tcaagttcac
     5701 ttaatttcgt catgttcggt atctcctact gctggataaa gcataattga ttcatagttt
     5761 tctttttgtt gcgcaatgat ctgccctttt ttcatcaatt ctagcaaagc cataaaagta
     5821 gtgacaactt cacttttaga aggtgactca aaaaaagaat cgaaagtaca gccatgcgca
     5881 tttcttttta tacgctcttc gataaatacc attcgttctt cgatcgttgt gtcatcgggc
     5941 gtgatcgttt tttccacttt ttgagaaaat ttcttacgtt ctaagatttt ctgcatggca
     6001 cggatcaaat cgattttttt gacttgtcct cgcttcaacg gcttttcagc atccgctaac
     6061 ccatcgatgt tcataggttc ttttgtgtaa taaagacttc gttcctctgc tttttctgtc
     6121 agtctttcag ctgcatattt atatttacgg tattctaaaa gctgagtgac caattcttct
     6181 cgaggatctt cttcccataa ttcttcatcg tctgtgacga attcttggac aggaagcaat
     6241 gtcttgcttt tgattgccat caatgtagca gccatcacta agtattctcc tgcaacagct
     6301 agctcgagtg ttttcattgt atggatataa ttcatatatt gctccgtgat cgtagcgatc
     6361 ggtatatcat atatatcgat ttccatttgc tgaatcagat gaagcagcag atcgagagga
     6421 ccctcgaata catctaattt aatattgatc tccgtcaact cattcactct ttcttctttt
     6481 tctttgctag ccattttcca atgtaaggct ctgtgatttc agttgcacgg atggagacat
     6541 ccgggtactt ttcctgtaat tcatctaaca tttgaaagcc catattctca ccgcgataag
     6601 acggattcaa agaaatgtca tggatgatca gctgatcgtt cgttgcccag taaataccaa
     6661 taatcccttg aatgttggtt gaaccttctg gcgtaaagaa aaaaagttca taatcgtctt
     6721 cagattcgta cgtatcaatc tccttcaaca aagaagtttt acctgtcagt ttcttatgga
     6781 aactcaatag tcccatagca attttacggt tttctttccg gtaatgtgtt aacatcttct
     6841 caccttccta tgactaagcc cttggaaaat gctgcttata aacatccgtc attcttttct
     6901 tcgtgatatg tgtataaatc tgagtcgtag agatatccgc gtgacctaac aattcctgta
     6961 ctgtccgcaa atcagctcca ttttctagca gatgtgtggc aaagctatgc cgcagcgtat
     7021 gtggtgttac ttctttgtaa atcccagcct ctcgtactaa ctgcttcaga tttttccaga
     7081 ttccttgccg agaaagacct ttcccatgat tattgacaaa tacatgcatt tcagatgcgt
     7141 cctttacaag taatggacgt gcttcgtcta agtagcgttc cagccattgg atcgcataat
     7201 cacctaaagg aatgatccgc tctttatctc cttttccaac tgtctgtacc agtccaagag
     7261 aaagatgaag atctcccaat ttcaatccaa tcaattcact gacgcgcatc ccagtagcat
     7321 acatgacttc caagattgca cgatctctta tccctaacgt ttttgtcgta tcaggtgctt
     7381 caatcagctt ttctacttct gtcagagtca atgtacttgg taatttctgt gctttcttcg
     7441 gcgtatcgat atgttgcatc ggatcatgat cagtgatacg ctcttgccgt aaaaactgat
     7501 ggaatcttct gagacttgag atcatccgag taacagttgc tgtcgctttt ttttcttcat
     7561 gcaacttctc cagaaaacgg atgactaaaa agcgatcgac atcctgccat gaagcaattt
     7621 tctgctcctg tagaaaatgc aagtactgtt ccaaatcacg ttcatagctt tgtcgtgtat
     7681 tggcggacaa tccccgttca atattcaagt agtggagata atcgttgatt tgctctttca
     7741 taataatccc cctcatgcct ctctatcata tcagataccg cttcattctg atagtctttt
     7801 tccataatct gagcaaagaa aaagccgtct agacactcag acggactggt ttcttttttc
     7861 tttttcatag cattcctgac agattccatg gaacgttagc cgatgatctt ttactaaaaa
     7921 atgataacgt tcttctacta cttgctccac ttcaagtaaa agatcctctt ctacttcctc
     7981 gatcgtgccg cagttcaagc atagtaaatg atgatggaaa tgtttggcgc cttcttttct
     8041 caaatcataa cgggctaatc catcattaaa acttacttta tcgacaattt tcaattctgt
     8101 caagatttca agggttcgat aaacagtagc aagtccaatt tccggactct tttttttcac
     8161 taaaaagaag atttcttctg cagataaatg ctctttctca ttttccaaaa gcacaagcag
     8221 agtagcttct ctttgcggtg tcagcttaaa ccccgcttca tgtagctgct gttttgtttt
     8281 tttcatcgcc gaaatagcat tcatgaccat ttactcaacc ccaacaattt ataataatta
     8341 caaataaaaa aagatataac actatgttaa ttaaaattat tataagctag ttaagaaaaa
     8401 catgcaaggg attctcaatt agattttttg actagttttg tcacaccgtt ttcttgtgta
     8461 atcaatcctt gtttcaaaag attacctaat gcgcgcttaa atgctccttt gctgatacca
     8521 aaagctttca tgatttcttc tggatccgat ttatctgtaa agttgatttg ctgatctgca
     8581 gaacgttcta aaatggttaa aatcatttgt gcatcatctg atatagcttc atatgctctt
     8641 ggcttcaatg aaagattcag tacaccgtct ggtcggacac caatcactcg accttttatc
     8701 tgctcgccta gacgaggttc attatatcgt tcagaaggat ggataaaccc aagatagaaa
     8761 tcttccgtta aaacgtacgt acctgttaat ttcaagcggt atgctgttcc tatcacatct
     8821 ttgtttttca tttcttctgt tccatatttg cttaaagatt tgaatatttt ttcatctgct
     8881 aaactgcccc aaatacggtc ttttgcatca actctaaggg aaatcatcaa gcgatcccct
     8941 tttttaggcc atagctctgt cattgtcggc aattcatcaa gagatacgac aaagtcttta
     9001 tctggaagac cgatatcgac aaacgcacct aaatcttttc gtgtagcagt aacgattcca
     9061 aaagcgtaat gaccgatacg gcttttagga atttgtgtag tgatccgatt ttcatgtttt
     9121 tgattttggt aaccgaatcc ttcaactgct tcaccgattt tgtgttcacc ttctgatttg
     9181 cttaagccaa aggtttgtcc gtttttttgg acaaagtagt ttttttcatt ttcatcaata
     9241 ataatacctg taaatatagt tgctaataaa tcgttcattt ttttctccct ctatagtgaa
     9301 aggttttctt aatacatttc tttgctatca taacataatg ttatctactt gaatagctga
     9361 gccttggttt ggaaaggtga aaaaaggagt atgacaacag ttgtgtcata ctcccctttt
     9421 tcgaataaac ggtgttaaac ggcaggctca cagcctcttt catttgccta aaattaattg
     9481 ctcccatttc ctccgaacca tccagtttta cagttgatgg ttacgatgta aaatggcaaa
     9541 gcactttctc caatgccttt catcaatgca gattttgttg aagtggcata tagattataa
     9601 tagccttccc cattttcgat tctggctttt tctaatctgt aacctccttc aacgatatat
     9661 ccacgttgga tacatgtagc aatcactttg ctttggacac ctggtgccca cgtccatgct
     9721 ggattagatg catcggcaat atcacttgct ctggcagatt tgattggctg attcgctgag
     9781 tttgaagagg aaggggtcga ggatgccgct tcttgctgtg ctgcttgtgc gaccgcagct
     9841 tgttgagcgg cttgagctgc agctgctgct tgagcggctt gttttgcttg ttcttcagct
     9901 gcttttttcg cttcttctgc ttttttcttt tcctcttctt cttttttctc tttatcagtg
     9961 agcatcttat ctacttgttt caattgttca ttcaactgag cagatagatc tgtatttttc
    10021 actttttcta ctgcttcttt tgccgtcttg tattggtcgc gtgttgctga atccactact
    10081 tttccgtctt taaagactat agctgttttt tctttagctg tttgcaattc agcatattga
    10141 tttttagctt ctgtttgtgc ttgttgaatc aattttccaa aatctgtgtt atccgtttcg
    10201 attgcttgga tctctttatc tgctttcaac ttgatgtcat ctgctagatg atcatcgacg
    10261 atggcaggtg ctatgaacca ctcattcact ttttggatat ctcggatctt agattggata
    10321 tcttggtaag ttgtttctaa ctctgaatat cctttttcat tcttaagact ggataaatca
    10381 ttttttagtt ttgataaatc ttgatgaatc atatctgcac gaataaattg atggtgatca
    10441 tctgtataaa aggcagcaag tttctcatga agttcttcca tttgttgctg tctttttgct
    10501 tctgctgcta cttcggcttc tactttttga tgatggtcat aataagccca gcttcctcca
    10561 ccgagtagaa gtactgctgc taaaacgaga taaggtactt tttttgattt tttcttttgc
    10621 gctttatctg gatctgtttt ttctaagttt gattcttgat gtgttttttc agtatctggc
    10681 acgattgtta ttgatttcgc ttctgcttca aacagcttag gctggttctc ttctttttct
    10741 atttttgctt caagatcagt accctctttg cttacagatt gattttcagc atcggattca
    10801 ctatttattt catcgggctc tgttgacttt ttgtctgtgt tttcgagtat ttcttttttc
    10861 tctgcttccg gcagtttatt cgttttttcc ttttctaatt gtttttcagt aaatacttca
    10921 gttttttctg tgatatccgt ttttacgtct tttattttta ctgtttcttt acctgtggcg
    10981 ttttctctat cagagtcttt ttcagtttct tgttcctttt ctggctctac tttgttttcc
    11041 tgtgcaattt tttcactgat cattttgagc agctctttcg cgtgctcttc ttttgtatct
    11101 tcacgatgtg ctttgatata agcggatagt atcgaatttt cttcctcatt atcgacgatt
    11161 tctttcagtt cagctactct tcttttttgt tttgattcaa gcgtttcttc ttgattcttt
    11221 cctgattctt ccgaagtctc atgaacagtt gctttcttct gttcattttt ctctatatta
    11281 gttacttgat cgtgagcagg tggttcttcg tcttctggtt ctgctttttc gaataattcc
    11341 attacagatt ctagaggtaa atctttcaat tccgaccatt cgattggatc gtttaattct
    11401 gtatcaggaa agactgcatc ttcagttgat ttatcctgca tagctgcatc tgtatttgtt
    11461 tcttcaattg attcttttat ttgctgatct tctgcagtta tttgatgacc gcagtctgga
    11521 cagatagtat tctgctccat ttttttcgat ccgcaatttg gacatttttt catcacttag
    11581 cgatcggctc ctttactagg tttacgctac catttaatct atcatattct atacgtcacg
    11641 ccaatagctt atttttagga tttacttaaa aataatgaat gaatttcctt cctatagaaa
    11701 aaaaagcttg agactgcgta gtctcaagct ttttttcttt taaaataaac caaacagttt
    11761 tactgatttc tcttctggtc aatagtccac gcttattcta agaaatcttt taattgtttt
    11821 gaacgagaag gatggcgcaa ttttcttaat gctttagcct cgatctgacg aatacgttcc
    11881 cgagtaacac caaatacttt ccctacttct tccaatgttc tcgtccggcc atcatctaaa
    11941 ccaaaacgta agcgaaggac attctcttcc cgatcagtca atgtatctag tacatcttcc
    12001 aattgttctt ttagtaactc ataagctgcg tgttctgctg gacttgttgc ttcctggtct
    12061 tctatgaaat cacctaagtg agagtcatct tcttcaccga ttggcgtttc aagggaaaca
    12121 ggttcttgcg cgattttcaa gatttctcgg actttttctg ttggaagatc catttccgca
    12181 ccgatttctt ctggcgtagg ttctcttcca aggtcttgca gcaattgacg ttggatacgt
    12241 atcaatttat taattgtttc aaccatatga actggaatac ggatcgtacg tgcttggtca
    12301 gcgattgctc gagtgatggc ttgacgaatc caccatgtag cataggttga aaacttaaat
    12361 cctttgcggt agtcaaattt ttcaactgcc ttcatcagtc ccatgtttcc ttcttggatc
    12421 aagtcaagga actgcatgcc tcgtcctaca taacgtttag cgatacttac tactaaacga
    12481 aggtttgctt ctgccagacg ctgtttcgct tcttggtcgc cttcttcgat tttcaatgct
    12541 aacgctactt cttcttccgc agttaaaagg gagacacggc caatttcttt cagatacata
    12601 cgaaccgggt cattgatttt tacaccggtt ggcgcagaaa gatcttctgt ttttgcttgt
    12661 tcagcttttt tctcagcact cttcaagctg tgaattgatg gatcaccatt ttcatccacc
    12721 acactgatac ctgcgtcttc aatcttttgg atcaatttgt ccatttcatc tgcattcaaa
    12781 gtgaatggtg tagcaagctg attggataaa tcatcatata caacttgtcg tttgggtttg
    12841 ttttctttga tgaaagctgc tactgctgct tcatacgttt ttttatctgt tccatttgcc
    12901 ataaaagaag gccccccttg agtagctatt tggcttgttt taactgtttc gttaaattga
    12961 tgatttcaat cgtcaattca agctcaagct gtttgtttcc agttcgtctg gcttcttgct
    13021 gcatttgttt ttttaatgtt atttcatcag ctaagccaga tttgctgatc acatctagca
    13081 agtcttggat ttctcgtggc gaactttctt ctgccaccgg taaatattcg atttccacca
    13141 ccaaccgcct taaattttca tcttgcaaaa agtctaaaaa ctctgcaagg ttgaattcat
    13201 aatgttgtga gacatatgca tcgaataaaa aataaacttc tgcatatgca tcatgaataa
    13261 actgaaattc tcgttctttt aaaatattac ggacagaaac ttctttaaat gcacgatata
    13321 atagaatctg ctctgccttt tctacttgcg tcaaaggtct ctttttccgt gcagtttctt
    13381 gtctcgtaac ctgagtgtgt tgactttcct gaggttcttg acgattttgc tgatttgtcc
    13441 ttcgaagatc gcgcagttgt ttttgtatcg tctctctgcc gatctgaaac tctgttgaca
    13501 gttgagatag atacatatct tgttcgattg gtgaaacaac tcggctaagt tcaactagta
    13561 attcttcgag gtattcgatc ttgtcttttt cattatctaa atttttccct tgttttaaat
    13621 aacgtgattt aaaagtaaag actgtttcgc gcccatgagc cactaattct tggaaagatg
    13681 cttctccata cttacggaga tattcatcag gatcaagctt ttctggaacg actacgacag
    13741 aaagtgctaa tcgactgttt tgccgaagca attcaatccc tcgattggtt gcttcaaaac
    13801 ctgcgttgtc tccgtcatag caaaagacca attctttcgt cagcttttcc atttgttgga
    13861 tctgttgagc tgttagactt gtccccatag aagctaatcc agttttgata ccagcctgcc
    13921 atgcagcaat cacatccata aacccttcga acaacaaaat ttctccgttt ttccggatat
    13981 cgcttcttgc tttatcaaga ttaaataaaa cttgtctttt attgaacagt tctgtttctg
    14041 gactattcaa gtatttgggc tgatcttttt cctgcccttt ttcaggtgca agccagcgtc
    14101 ctgaaaaacc gatcgtctgg ccttgagaat tacgaagtgg aaacatcact ctttgataaa
    14161 aacgatcaga catacggcca tcatcatgcg tgacaaacaa tccagagcgt tctaacaatt
    14221 catttgaaat tgattcattc ttgaatactt gctgaagaaa agaacgctca ttaggtgcaa
    14281 aacctatgtt gaattcttca attaattcta aagacaaccc acgactttgc aaatattcta
    14341 aagctggctg tccagctttt gtatgcaata gcatgtgatg atagatttcc gctgcttttt
    14401 catgagcagc aatcagtttc cccgtagcag attcaggatt ttggacagct atagtacgct
    14461 gacgccattg atcttctaac ggtatctgtt cgaagtctgc cacttttacc actgcttctg
    14521 gaaaacttag accttctagt tcttgaagaa aagtaaacac gtttccgcct ttaccacaac
    14581 caaagcaatg aaaaatctgc ttgtcttctg caacagaaaa agacggtgta cgttcttcat
    14641 gaaaaggaca cagccccaag taatttttac cagacttttt caactggaca tattgtccaa
    14701 tcacctcaac gatattcgtt tgactgcgta ctgtttcgat aacctcttga ggaatccgtt
    14761 gagccatttt ttcaccccct gctctattga tatcttttgg gtttaacaga agatatttca
    14821 ggagaataac cggtaaaaaa tcgcactaac tgaaatcagt gcgattttaa aggacacaat
    14881 tatacatgtt tgattgtatc atatttcatt gctttttcaa gtataacaca tttctatctc
    14941 attaagcatc aatttgctac tcttttcagt aactgatttt gaatatcagc cttccagtac
    15001 aatactcgaa gaacaatatt tacgagcatg aatacaaaga agtatcctac gctctctccc
    15061 aaccctaagt ttacttccaa aattccatgg aacagcattg cagctacata tatccctacg
    15121 atcgtcgtta ctgctaggaa gatgcgcaat gggttgaagg gataacaagc ttttacaact
    15181 gccatgcaac tgatcccaat cagcaagtaa tacatcaaag tagtagtttc agcatttccc
    15241 catgcgaatg tttttccaag tagatacacc gcaatgatat tcactactac aagcaaggca
    15301 tttggtaaag cattaataag tgctgtaggc aaataacggt aagtgatttt tcttttatct
    15361 tgttcaaaag ataaaaagaa ggaaggatac ccttcaatcg ctaaatcaat cagcgtgatt
    15421 tggataggga taaacggaaa tccaattgct gtaacggcac atataatcga taaaataaat
    15481 gaataaatcg ttttgataaa aaagatgcct gagactttcg tcacattgtt gaccactcgg
    15541 cgtccttcaa acaggacttc tggcaatgta gtaaaatcag agtccaacaa taccagattc
    15601 gcaatctgac gtgttgctcc atcaccttct gccatagcaa tgctgcaatc tgcttctctt
    15661 aacgccaaaa catcattcac accgtcgcct gtcattgcaa ccgttctgcc gctttctttc
    15721 aattcattta ccagcaattt cttttgctgc ggactcactc gtccaaaaac cgtatagcga
    15781 tgagcagctt ctcgcacttc catttcttca gtaatttgag aaagatcgac atacgcatca
    15841 taattcggta atcctgcacg tctagcgata ttggagacag taaaaggatt gtctccagaa
    15901 atcactttca gatccacacc ttcctcatga agataagcta gtgtttcttg agcgtttttt
    15961 cggattggat catcgatttc aaacagagca accggctcaa caaatgctgg tttttcttcg
    16021 ttcaattgct gatttggagc gatccccaac ataagtacgc gatagccatt cgcttgggct
    16081 gtttttagac tgtccggaag ccgatcttct tccaccaaac gttcaggcgc tcctacgtac
    16141 acgactccca gttctggaaa ttccatcgcg ccccatttgc gatcagaaga aaatgcgact
    16201 ttgtttgtca catcataccg attagaaagc tcaaaataat ctctcatagc ccgcatagtg
    16261 atgttgttat cactgctttc tgccagatac gtccccataa tttctgggat ggctttctcg
    16321 tacagcggat gtagtgcttc tacccgctgg atactcattt tcccttcagt aatggtccct
    16381 gttttatcca agcataatgt atcgacatgg gccagcgttt caattgaata catgtcttga
    16441 acaaggattc gtttttttgc cagtttggtt acccctgtcg ttaatgcaat acttattagc
    16501 aataccaaac cctttggaag catgcctaac aaagctgctg cagatgcaat aacagaaatt
    16561 ttgatagaag catcgcggat gaacagtgct tccaaaaata gtatgatccc caaaggaata
    16621 atcacgaaac tagtgaattt ggatactttg cgaatcgaat ctacaagttc agaacgaata
    16681 gctttgtgag tctttgcctc gctagtcaag cggacagcat aattatctgc acctactcgg
    16741 ataacttcag ccatgacaga accgcttgtc aaaaagcttc cagaaagcaa ttcagctcca
    16801 gtttctttga caatcaaatc agattcgcct gtcaaaagag cttcatttgc ttctgcccgt
    16861 ccagatataa cctgtaaatc ggctggtatc tgttctcctg ctccgatttt taccaaatct
    16921 cctaatacca gctctttagt agaaatcgat tgttctttac cgtcccggat cactagtact
    16981 tctgattggg aaacaatcga taatttccgt acaagatttc ttgcatgtat ttcttgataa
    17041 attccaataa cgatattgac gacaataatt gctaaatacg ccatgttgga atacgcacct
    17101 acaaataaca gacaaatccc aataccaaag ttcagaaaat tgaataacgt caatatatta
    17161 tcgctgaata tttgttttgt actttttgat gcatcttcct ggtagtcatt ttgctggcca
    17221 gaagccattt tctgaactac ctcttgctgt gtaagtccat tcattttctt tttctccatt
    17281 ttccgccaac tttcaaaaat aatcacgtgt ttaagtctag cgtgttccaa gattaaaaac
    17341 aacttgtttt tataaaattt atagaaaaac acacgaatga ttttacaaaa aagatgccaa
    17401 cctttttgtt tgaatcaccc gtgtgctgtt tttattatca atctatcttt acttatattg
    17461 ttcctccaaa cggctcatcc accaacctag tgctgtaccg gctataaaaa gaacaggaca
    17521 agctagtacc ccagcaactc ccaatcccgc gtaatcggta ggaatgatca taatcgttga
    17581 agcaaaaact acacctaaaa tgaaatggaa tagcttgcta tatgcctttg agaaaatgta
    17641 atccatgatt tttgacaatc caagaacaca aagaatccca ccaattccga tcggcacaat
    17701 cacttcaagt cgtacttctt taaatccgtc agccatcgct ttatacaatc ccatatacac
    17761 caaaaaatta gaagggctca atcctgggac gatcataccc aaaccaatca atccgccggc
    17821 aatcagccat gttccaaaat tctgatcgac ttgcccgtca aataattgag cgccgtataa
    17881 taaaaacaaa taagccagaa caaaagtaac aaccatgatg atcacatcag aagtatctct
    17941 tccttttttc cctgcttctt tccataacgc aggaacagtt ccagcgatac atccgacaaa
    18001 aaaccataat atcgttgttt catacgcacc taataaaaaa ctgactaaaa aagaaagtag
    18061 aaagatgcct gtcactccgc ctagaccaac aggcagaaaa aacaaaacat tttctttgaa
    18121 attttttgta aggtgggcta aaaaagaaat cagccgctca tacatgccaa agacagctgc
    18181 taatgcacca ccgctgactc ctggcaagat aaatcctgaa ccgataaaca ttcccttcac
    18241 aaaccgtaac atccagtctt tggttgtgta ccccacttct tgattttcca ttttttgcct
    18301 tttttccact atttgcgcac tccttctttc atttaatttt attcatgatt gaacatttcc
    18361 tgctatttta cttaaagact ttttcatagg tctctattat tttttcattc attttgacaa
    18421 tatcaggata aaacatgtta cctccattat gatagagata gccgccattg taaagcaagg
    18481 actggatccg ccaatatcgg tattatttcc cttcctcatt tcctaaaagt gggctcagca
    18541 catctttaga atacgcttct gctaattcta ctgtgttttc tccgccatga ttttttacat
    18601 aggcaatata atctttccca aaattatatg cttggacagc tgtccataaa tcgcatcctt
    18661 gtttctttgc ttctactatc atttccgcca aatatttgac accttgatca atactgtcag
    18721 ctggattatc aatgatctca gcttctccat gcagactttc cgagctctgc atgagatctg
    18781 tcccttttcc ttttgattcg gtcaatatta tcgccttgat caaattctcg tagttttcca
    18841 tatgataatt ttttgcagct gttgttattt ctatttgata gctttccact cgttttaccg
    18901 aacgataagt tcttcctcct aaaaacaaca ccgcaccaat aaccgtcaga atgatcatgt
    18961 aaaaaagaat acggataata gataatcgtt ttcgcttcat ttcatcacct agttattttg
    19021 acatcttttt ttatcttact atttctttat cttcttggct atttcttcct tgatttctgt
    19081 gtacgcctta agaaaaaatg aattttcctg tattttttga catcttacga aaatgatcgt
    19141 tgtttttcag taaaaacaat tgcccaatga ctgatataat cgttatactt tacacagtag
    19201 gtgattatgt gaaaaaagaa aagaaatatg caaaaatgcc agatgggagc gaaatttact
    19261 atgaaaagag cggtcaaggc ttccctctct ttttactgca tggcaatgac ggcagcggcc
    19321 gctttttttc agaacaggtc cctgtactcg aacgctacta taccgtgtat ctagtagata
    19381 gccgaggaca tggtcgttcg acaaacgaag catctatgtt gaattttcag ttaatggccg
    19441 aggatctgaa tacgatcatg ttgctggaaa aaatcgatca ggcagatttt cttggattta
    19501 gtgatggagc aaatctagca ctggtatttg ccagcagctt ccctaaaaaa gttcatcgat
    19561 tgattttaaa ttctggaaac acgcttgtta aaggcgttcg tttttctgcc agagtcatca
    19621 gtaatattca ttatgcatgg gtatggttac tctctttatt tcgcccaagt cttcgcaaaa
    19681 atcttttagt tatcaaactt ttgctgcatg acatcgggct tacagaaaat gatttaaaaa
    19741 aaatcaactc ccccactttg atcatcgtgg gaaaaaaaga tgtaatcaag ctaaaacatt
    19801 ctttgtatat agctaaaact ataccaaaag cttcctttgt gcttgtgaaa gaacaaggac
    19861 atgaattagc tagaaaagat cctgaacgct tcaatcgaga agttcttcaa tttctttctg
    19921 aaacataact attaggaata gaaggtgatt tatttgttaa aaaataccat acaatggctg
    19981 aaaaaccacc taggactgtt caaaacaatc tttttgattt ctgtcattgt cattattgtc
    20041 ggtgaattga tgtccatcgg taaaactcta tctatccagc aattaggaga aacgtttgcc
    20101 actatacctg tatggaaaag cggactaatg ctattgattg gtcttatctc tgtgttacct
    20161 atggttggtt atgatattat tttgaatcgg atgcttgaac aaaagcagaa tcctcgttac
    20221 ctgtttgaga caagctggct gatcaatacg atcaacaata ttgccggttt cggcgggttt
    20281 gttagtgtgg ggcttcgctc tgaactatat ggacaaaaga aagagagcag aaacgtgatc
    20341 caagcattat cgaaagtttt cttgtttttg atggctggac tttctattta cagtctgctt
    20401 tcttttttac ttgtagtctt cacgccagtt gctccttttt tgaagcaata ttggatatgg
    20461 ttgatcggcg gcggtctata ttttcctgtc gtctttttat ttactacttt caaaaagaaa
    20521 ggattccttg gtggtacctc acttaaaact caaggacagc tattgctagt ttcattgttg
    20581 gaatggaccg gtgtcctgtt tagtttcctt tctgttggtt atttaatgga aatccggatt
    20641 gatctgtggc aaacaattcc attattcatt gctgcttcag tgataggaat cgtgtctatg
    20701 attccaggag aaattgggag tttcgatgta atgatgatta tcgggttgtc cgctattggt
    20761 gtgccaagag aaatagtcgt tgtgtggatc ttgctctatc gcctgttcta ctacatcatt
    20821 ccgtttttga ttggaatcgt ctttttcttc aaaaatatcg gttccacttt tgatcagcgt
    20881 tattctggta tccctaaaca attagcaaca gaaattgcgc ataaaatcgt ggttgtctta
    20941 ttatactttt ctggaatcat gctagtactt tctgccacta tcccgcaagc ttttacagag
    21001 tttcgctggc ttcatagttt gaacccattg aaatttcatt ttattatcca atttcctagt
    21061 atactattgg gatttctttt aatcattatg ggcagaggga tcgcagcacg tgtgaaacga
    21121 gcatatttgc ctacaatctt cttgattgtt ctagctttac tttatgtttt attaagtgat
    21181 tttagtttca caccggtggt tttcttgtct attctgcttc tgatcattct tgcttctaaa
    21241 aacgaattat ttcgggaaca attgatctat tcatgggagt ggcgtacgat cgatggaatc
    21301 attattggtg cactgtcctt gctttacatc gttattgggg tatacaatct gccagatttc
    21361 cctcatcggc gtcaccattt tatttccttt ttcttatttc cttctgaaaa gatttggttt
    21421 tcgggattgt tggccatcat cgctgttagt ttcatgatcg ttctatttgt tcatttcctt
    21481 caaggagaaa agaaacagat tggcgaagcc tttaatgaag aaaaggccct gaagatcctt
    21541 acaacttatg gaggaaactc cgatagtcaa ttgatcttct taaaagataa gcggatgttt
    21601 gcctatgaaa aagacggtga accaactgtt cttctccaat tcgcttgctt caataataaa
    21661 tgtattgtaa tgggtgatcc ttctggtaag aaagaagatt ttccagaagc aatcgaagct
    21721 tttatcgaag aaacagatcg actatgctat ttaccagttt tctacgaaac aagcgaagag
    21781 attgttatga ttttgcatga attcggctac gatttcatta agatggggga agaagcttat
    21841 gtagacctga atagttttac aacttcaggg aaaaaaatga agggcactcg tgctgtcttg
    21901 aatcggatcg aacgagaagg atttacattt gatgtcttac aaccaccatt ttctactgag
    21961 caaatgagta ctttcaaaaa tatctctgat aattggttgg gttcacgaaa ggaaaaaggg
    22021 ttttctttag gctttttctc tgaagattat ctacaaagag cgcctattgc agttgtcaaa
    22081 aatacccaag aagaaattgt cgcttttgca acgatcatgc ctacttacac gaacaataaa
    22141 gtaggaacga ttgatctgat gcgatatgat tctaaaaaag caccttctgg cagtatggat
    22201 ttcttgttct tgcatttgtt tgcctatatg aaagaagaac atatccaatg gttcaattta
    22261 gggatggccc ctctttcaaa tgtcggaact tccagaaaga gttttttaca ggaaaggatt
    22321 gcatacttgg tttacgagtt tggttctcat ttttattctt tccatggatt aagagaatat
    22381 aaaagcaagt acgctacaaa ctgggtctca cgttatactc tatattctag agatagttgg
    22441 attgcatatg ttatgattgc tttgctgatt attgataatg cgccagttga gcagacacaa
    22501 ggtagaataa gtggtttgaa aaaatggata cgaagaaagt attaaaaact gcataccgat
    22561 agatgaacac tctcatgatg aataaaagag actgaagtaa aacgaaagtt catgcttcta
    22621 caatctcttc ttatacaatt gaagaacaaa gagcggtcca aacagaaatc ctgtttggac
    22681 cgctctttta ttttaaacat ttttcattac caaaatattc gagtcgcgtt tcttatgctt
    22741 cgatctctgt aacgatacct gaacctactg tccgtccgcc ttcacgaatt gagaatgtcg
    22801 ttccgttttc aacagcgatt ggatggatca agtcaacatc aatcgtcacg ttgtcaccag
    22861 gcatgaccat ttctgtatct tctggcaatg tgattgttcc tgtaacatca gttgtgcgga
    22921 agtagaactg tggacgatag ttgttgaaga atggagtatg acgtccacct tcttcttttg
    22981 tcaacacgta cacttctgct ttgaattttg tatgtggagt aatggaacct ggtttagcaa
    23041 gtacttgtcc acgttcgata tcgtcacgtt ggatcccccg taacaacacg cctacgttat
    23101 ccccagcttc cccgtaatct aacgttttac ggaacatttc tacacctgtt acaactgctt
    23161 tttgtgtttc aggtttgatc cctacgatct ctacctcatc accgacacga acagcaccac
    23221 gatcaatacg acctgaggca acagttcctc gacctgtaat tgagaagacg tcttccactg
    23281 gcaatagtaa tggtttatct gtatcacgtt ctggtgttgg gatatattca tctactgtat
    23341 ccatcaattc cataatagca gcttcagcat ctggatcgcc ttgcaatgct tttaatgcgg
    23401 aacctttgat cacaggagtg tcatcgcctg ggaaaccata ttcgctcaat aactcgcgaa
    23461 cttccatttc taccaaatcg atcaattctt catcatcgac taaatcaact ttgttcaaga
    23521 aaacaatcag gtattttaca ccaacttggc gtgacaataa aatgtgttca cgtgtttgag
    23581 gcatcggacc gtcagttgca gaaacaacta agattgcacc atccatttgt gctgcccctg
    23641 taatcatgtt tttcacatag tccgcgtgtc ctggtgcatc aatatgcgcg tagtggcgtt
    23701 tttctgtttc atattctacg tgtgcggtat tgatcgtaat cccacgttca cgttcttctg
    23761 gagctgcatc aatactagca tagtcttgag gatttgctag gccttttttg cctaatactg
    23821 tagtgatcgc agcagttaaa gtagttttcc catggtcaac gtgtccaatc gtaccaatgt
    23881 taacgtgtgg tttacttctg tcataatgtt cttttgccat aatttacaaa cctcctaaat
    23941 aatatagatc actgaaaaat ttcagctctc acctttttat tatagtcttt ttttgactaa
    24001 atcaaaaaga atacgtccga cttacttttt gtaagattcg aactaagctt attatacgtc
    24061 aatattggtc aaagtcaact aagagcactc gtctgtctta tacgcgaaaa aagtaccttt
    24121 tataaataaa aaaatgtgtt ttttgagaaa aacttcaaga tagtccttaa ctttcttttg
    24181 aagcaataag aacaaaatga ggaaaagttt aaaattcagc tgttgccaac aactttcaat
    24241 agacgaaatc ttctatttcg ttaataataa ggaaagaggt gaagtacatg aaaaaattaa
    24301 ttggcgtact tgtcgtttta ggcatatttc tcggcggaag ttttgcagct tatcattatt
    24361 tttacggcgg cgaagcttat tatacaaaaa tcacgacgaa tggcgaaatg tcagctaccc
    24421 aagcagataa cggagagaaa ttcacgactt atacctacaa gcaagcggct tataataaag
    24481 atggtgaaaa aaaacaggtg acgcttcgcg aagaaagaga acatccatta aagatgaatg
    24541 cgtatcttaa actcaaagtc aatcctcgta aaggtgtatt aagctgggaa gaaataaagg
    24601 cttctgaagt gcctaaaaat gcggcagaaa aaatcgataa ttagtaaatg gtcttaaaat
    24661 tttattagat aaaaagaggc tgggacagaa gcgtttaact ccaagaaata agaaggagtt
    24721 gcttctgctt ccgccgttta tacgtgttta gagcgtgaga caaaagtgtt ctttactttt
    24781 gtctcacgct ctttttttgt tatattcgtc ttttccgatc cttctcttga cacaattcat
    24841 ttaactgatc actggtactt gaacacctaa tattctagtc atttttataa acgattcaat
    24901 acttcatcca catttttgat catcacggat atcgtaccat ccggattatt cacaaattca
    24961 atcaaatctg ggttgcgata tacatctact ggtacgatca gttcgatccc gtttgatagc
    25021 ttgaattttt gttttccaaa ttttttctca gaaatttctt gtacttcttt tacaggaggt
    25081 gcttctggga caaatccttg ctcttgtact tcttctttaa aagccatttt tgccgtgatg
    25141 ttttccttga agactttttc cgcaatcatc tcatgatcca accgaccttt ttcttctatc
    25201 gtatcataga cagcttcttt caaatcagcc atgatatcaa attcttctgt ctcaaatttt
    25261 tttccaagat gcttggctac tttttggatc acttttacat tttcttctaa agaaggtgca
    25321 ggaacacttt cgatcactct tccagaaaaa taaagctctt ttttgccaga aaactcatat
    25381 tttttttcaa ttaattcgta ggtccaatca gtcaaattca cagcgaatgc ttcatctgct
    25441 ttttgtgttt tcgcccctag aattgctcga ttcaaaatca agcggttgtc gatccctgct
    25501 tcgtctgctt caacaaagtg agtgtaagcc tcacgataat tgactttcaa taaagataag
    25561 tacatcactg tatctaattc atacaagaca acaaatacat ctgcactagg tgcttcttca
    25621 ctttcttggt agatatcgaa ccaccgctga acgattgctt cacttgcttc cacaaaattt
    25681 tcttgagcga ttttaagtga ttcaacgatc atgctttctt ctgctagttg tccagttttt
    25741 gtttgagcag aagagatttt ttggatcttc ttttgaagaa aatcacgaat atattctttt
    25801 gtcagatcca gctctttttg tgaaaagact ggagaaccag tttctctgtc aataatatgc
    25861 agaattgctt tttttaaata gatatccatt ttctcattcc cttctgtctt agtttacgga
    25921 aaaaaaatca aaaataccag aatcaaaatc attttctttt aaacaaaaat ttcaagagta
    25981 gaaacaaaat ggattctttt tttcgaaaaa tgcgctacac ttatagttga acgaataaaa
    26041 aaggagctga aagtatgaat tcattaacaa gcacttatcg tctatcaaat ggttacgaaa
    26101 tcccggtagt aggattcggc acatggcaaa cacctgatgg ggatgtcgct gtttcctctg
    26161 ttaaagaagc gctggctgca ggttatcgcc atatcgatac tgcccaagga tacaagaatg
    26221 aagaaagcgt cggccaagcg attaaggaaa gtggaattcc tagagaagag atattcttga
    26281 caacaaaatt atggaatgca aaccacagct atgaacttgt gatgtcttct tttgaagaat
    26341 cattgaagaa gctccaaaca gattatttag atctgttttt gatccactgg ccaaaccctg
    26401 ttgcttttcg tgataattgg gaacaagcaa atgcagatac atggcgagca tttgaagaac
    26461 tgtatgaagc aggtaaaatc aaagcaatcg gtgtgagcaa tttcttaccc caccatctag
    26521 atacactagc aaaaacagca aaaattatgc cgatggtcaa tcaagttttc cttgcaccag
    26581 gcgaattaca gacaactgta gttgagtatg caaaaaaaca tgatatgatc ctcgaagcat
    26641 atagtccatt aggcactggt aaaatcttcg atgtgcctga aatgaaacaa atcgctgaag
    26701 cacatgacaa gacgattgca caagttgctt tgcgttggtc gctgcaacac gaattccttc
    26761 ctcttcctaa gtcagtgaca ccaagcagaa tcaaagaaaa tactgaatta tttgattttg
    26821 aattaacaga agaagaaatg aaacaaatcg atcagttaga tggtgtagtc ggcaaagcaa
    26881 aagacccaga tactacccaa ttttaagtcg aaaggtgatt ttatggatcc aatcgaacgc
    26941 ttgaattctc tctcggaaga agttacccag acttttcatt ccgattttgt ctttttgatc
    27001 gatgcagaga aaatccaaca ttttcctgca agaaactgga cacatgatca aatcatcgaa
    27061 gaactgaaaa aacggttcga tcattcgcta atggtcaaac cctggcacga acatgaagtc
    27121 atttattcac ctgaactacc tgtttttgcc ttgataccga aaaaatagaa agaatggaac
    27181 ggattatgta tatatacata atccgttcca ttttttattt ttctttcaaa aaacgggaaa
    27241 atacgcaaat ttttctgttg taatttgcgt atacgcaagt ttttttgcat tactcctttt
    27301 catttggctg tttttttctt ataatcgaaa gggtatgagc gaaggagaga acatatgaag
    27361 aaaaaacaga aacaaaaatt attaaatcaa ttccgtcctt cattggatgg tgtaagagga
    27421 caattattcc gttctctaga agaagagagc ttgcgacgtt atggtttgcc gttacaagtc
    27481 atgcttgatc ccaaaaaccg tcaaatctta acgatcggat tagctgggaa aacaaatgaa
    27541 gatacacgga tcaacgtgcc aatcgatgac aacttcaata ctgtattaaa gagaattcgt
    27601 tcaggagaaa acgggatttt cgaacgattc cgtgacaatc ttcttattga aatcgtgtct
    27661 tactggaatg atcaacgatt gaaaaacgat gaaccaactg cgcaagctgt tgcgacacca
    27721 gttacagaag aaacagtcag tgtgccagaa gaaaaaacgg aaaaaccaaa agcggacaca
    27781 actgaaaata cacgctcaac tgatacgaat gccgctttaa gttttgctga tttttcaaaa
    27841 gccgttgctg agtatcctaa attttacaca gaacaaaatg aacatgaagt agttattaat
    27901 gaaaaaaatg ctgatgaacc tcgtaaatta gcaactattt ctatgactac tgaaaatgag
    27961 ttcgttatcg aaaaagcact cgaacggaaa tacaaagtca aattgacttt gattcctttg
    28021 atcgaacaat ttgcagcgac agcgattact gatcgataaa aattctattt gtcataaaag
    28081 gctgggattt aaaatcccag cctttttagt catataataa tttctacagc aagtaaaaag
    28141 aaattccttc taacatcagg ctcaaatatc agcttgacct gtccaacaaa tcacctatga
    28201 tcctacaatg ttaggcgact tctcttctac ctgaagatat cttggagttg cctgatttct
    28261 taagatgcct aaccttaact ataaaaaatt ctaataataa
//
LOCUS       DACGLE010000032        28104 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0534
            SAMEA3304449-rid9560453.denovo.033, whole genome shotgun sequence.
ACCESSION   DACGLE010000032 DACGLE010000000
VERSION     DACGLE010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304449
            Sequence Read Archive: ERR985064
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 28104)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28104)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702225.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702225.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 20:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,045
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 27 of 67
            Pseudo Genes (incomplete)         :: 25 of 67
            Pseudo Genes (internal stop)      :: 19 of 67
            Pseudo Genes (multiple problems)  :: 4 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28104
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304449-rid9560453.denovo.033"
                     /strain="VREN0534"
                     /db_xref="taxon:1352"
     gene            complement(111..692)
                     /locus_tag="IXT20_001482"
     CDS             complement(111..692)
                     /locus_tag="IXT20_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAQ4859935.1"
                     /translation="MKTKEKILEATKDLIYKKGYHETSIRDILAASDTGKGQFYYYFS
                     SKKEVCLAVIHAHMDVWQTELFEDILNAGKQPEEALEEMLDWIFADHETQITYHGCPV
                     GNLIIELSTVDEEYRLLLLELVNQWVKYTKQNILALSEEKLSDEEADRQAKALIAEIQ
                     GSILLLKVTQDLSLLRQNFQLIKERLLTSQKVL"
     gene            complement(875..1501)
                     /locus_tag="IXT20_001483"
     CDS             complement(875..1501)
                     /locus_tag="IXT20_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAQ4859936.1"
                     /translation="MFNNKNTAVVLTDPQVEFLKPAGGGFGLTKDILEKYNTIENMIE
                     MVKQAKEKGYKLFISPHYFYPHDHKWQFGGAGEHMMLEQQMFARKSQYESVEEGSGAD
                     FIEELKPYLDENTIISSPHKIFGPESNDLALQLRKNGIDTVILGGMNGNLCSDSHMRE
                     LVESGFNVVVATDAIGAPGQEAMDAALTNFGFIADQVMTTEEVLEQLS"
     gene            complement(1523..2854)
                     /locus_tag="IXT20_001484"
     CDS             complement(1523..2854)
                     /locus_tag="IXT20_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-containing oxidoreductase"
                     /protein_id="HAQ4859937.1"
                     /translation="MMEKYQNIVIGFGKGGKTLAKALASKGESVLVVEKSTRMYGGTC
                     INIGCIPSKSLIFNGERGIDFTEAVARKEKLTGMLRAKNYHMISDEATGTVMDGTARF
                     LSNHQIEVTNNGEKVIVEGERIFINTGSEPIILPIKGLNTSRYLIDSTQAMDQEQLPE
                     KLVIIGAGYIGLEFASMFNEYGSKVVVLDAHSEFLPREDEDIAQMILEDMTNAGIEFH
                     LGVSVDQVADQESTAAVTFTEDGQEVTIQASKVLAATGRKPSTASLGLENTDVKLTDR
                     GAIAVDEYLRTDADNIWAIGDVKGGLQFTYISLDDYRIIMDQLKGENKRTTNNRKAVP
                     YSVFITPTLSNVGLTEKQAKAQGVNYKLFKYMTSGVPKAQVLEDPKGVFKVLVDPETD
                     LILGASIYAEDSHEVINLISLAMNGKLPYTLLRDQIYSHPTMSEALNDVLK"
     gene            complement(3009..3410)
                     /locus_tag="IXT20_001485"
     CDS             complement(3009..3410)
                     /locus_tag="IXT20_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HAQ4859938.1"
                     /translation="MKKAYETAMINHGGRNGEVEAPNGSMHMKITPPGIHAEGTNPEQ
                     LFAAGYASCFNGAVQHMIKEAGMTAESTVKARVSLYNLDDGGYQIGVVLEVHIDGISE
                     EEAQKIAEDAHEFCPYSKATRGNIDVEVTIV"
     gene            complement(3496..3702)
                     /locus_tag="IXT20_001486"
     CDS             complement(3496..3702)
                     /locus_tag="IXT20_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4859939.1"
                     /translation="MVTNMVIFLTVLVYGVNFLVYMYLKINHIKDYVEKIAIYFGTNM
                     TLLFTSSIFLFFGKIVEDGILVFE"
     gene            complement(3797..5299)
                     /locus_tag="IXT20_001487"
                     /pseudo
     CDS             complement(3797..5299)
                     /locus_tag="IXT20_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293678.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
     gene            complement(5317..5772)
                     /gene="argR"
                     /locus_tag="IXT20_001488"
     CDS             complement(5317..5772)
                     /gene="argR"
                     /locus_tag="IXT20_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HAQ4859940.1"
                     /translation="MKKKQRQALIRQIITEQPIGTQEELLARLHEAGADVTQATISRD
                     IREMKLVKSQNENKIVRYTLFNQPSVSLNEERLRTAIRREVLRIQSVQFMVIVLTERN
                     GADVVTNWLDEVAYPEVVGTMAGVDTFIIICRSEEEAQRFAEKLEKMRE"
     gene            complement(5870..6487)
                     /locus_tag="IXT20_001489"
     CDS             complement(5870..6487)
                     /locus_tag="IXT20_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4859941.1"
                     /translation="MEKIEWRKDKSYSMKKSPQILEVPKKSFFSIRGIGDPNQEDFKR
                     RVACLYAVSYAIRMSPKKNWLIPDYQMYTVYPLEGQWGLQEKYLHEPVMKKEHFSYQL
                     MIRQPDFVTEAIAQEAIQRSSKLPEDLKEQVVFKKMEEGLCAQILHVGPYAEEPATFE
                     KLEAFIAKEGYERASKEHKEIYISDPRKSAPEKMKTILRVKIEKG"
     gene            6708..8081
                     /locus_tag="IXT20_001490"
     CDS             6708..8081
                     /locus_tag="IXT20_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HAQ4859942.1"
                     /translation="MTKQKQLLLVPAVIATGMMSFAGVLIETAMNVTFPTLIRQFDLT
                     TAQVQWVTTIYLLMISIIVPLSTYLNRNFSLKKLFLASNLLFLAGIMIDFFSPTFMVL
                     LFGRLLQGAATGIALPLMFHIILTFAPLNRRGAMMGVGTLTTAIAPAIGPTYGGIMTS
                     HFTWNHIFLFLVPVLFLSLIIGWFAIPEMAVQKSGYLDWISVLGICLLFSGSLTFLST
                     LGTVSSWIALIIGGIGWFLFYYRSKKVEHPLIRLSILSNKTFRLFLIGFLVYQFLLLG
                     VSFVLPNFLQIVQGSSPFVAGLAMLPGAAVGALLSPFSGKMLDQYGPKKPIFAGLILS
                     LIGWLALFLLIGHASVGVLIACHVFYMIGIGLSYSNMMTTGMNALNEELQGDGNAMFN
                     TLQQFSGAVATSLVAVIINYVQHHTSHNYEVSTTLGSKAALGVLLLLLLVSFARFAYY
                     LFFAKKA"
     gene            8156..9268
                     /locus_tag="IXT20_001491"
     CDS             8156..9268
                     /locus_tag="IXT20_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HAQ4859943.1"
                     /translation="MEKIAIIGGGIIGMTLASYLDTSKFNVALFDEGTGQATKASAGI
                     ISPWLSKRRNKQWYRLAKDGAAFFPKLIKDFQLDQTIYAQSGTLLLRPEKDLAELAVL
                     AEKRKEEAPEIGNIEWLSPEQTSQALPLLKPAVSLRISGGGRLDGKAYLEHLGQRLAQ
                     KQMEQIQAKVTLHKKSDSWLIKGPFGEYTADRVILTPGPALKPLLQELGYAADIRPQK
                     GQLLAFQTSYKMSQDWPVAMLDGEADFIPFMDGKILLGATHENDQGWDLAPTSEAYKG
                     LTKSTEQFLTDPDQIKNWPYEFRVGTRAYTSDFSPFFGVLPDDPSLLAASGLGSSGLT
                     TGPYIGYLLANFLNTGHWPTEDYQKDISTYITKNPS"
     gene            complement(9340..10977)
                     /locus_tag="IXT20_001492"
     CDS             complement(9340..10977)
                     /locus_tag="IXT20_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HAQ4859944.1"
                     /translation="MSTKESLELNEITSGNPLLSSIRSIVETTFYGNNVHEVFDRKTA
                     YQLAKGSPGTIITDLTISHAEELDLPADVRTLVFNDGSIVGRTASARRIFEDLDKEQS
                     KYEKILREAVYQSRKRQFYHTKVIVGLSEEFSVQSHLLIPEGFENNLYSYLLNFQPAN
                     SKILETYQKSVSFDEGDLYIFCDPEWTHETFPNGLILFDALHNTAAVLGLRYFGELKK
                     ATLTLAWALGNRNGFIACHGGMKQYASNDQTYTMAVFGLSGSGKSTITLAKHTEGHSV
                     SILHDDAFVISRKNGFATALEPSYFDKTQDYPSDDPAISSFLTCQNVGVTCTSKGEKV
                     LVTGDIRNGNGRTVKSRYATPDRVDHLSEKIDAVFWIMKDDSLPPIIKINDPVLAASF
                     GATLATKRSDAENITTKRQLDELVIEPFANPFRVYPLREDYQAFRELFLKQQTACYVL
                     NTGYFDDKKIKPETTLSIVERIVQQTAEFKSFGPFEDIHYLPIPDFPVSFEDTSYKNL
                     VKQRLDIRLRFIEAQIGKDLLPDEAKESLENLIQKLN"
     gene            complement(11145..11753)
                     /locus_tag="IXT20_001493"
     CDS             complement(11145..11753)
                     /locus_tag="IXT20_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAQ4859945.1"
                     /translation="MNSFKKIVLGTTFAAGATAMFVGTTNAHADEVYTVKSGDSLSKI
                     SQKFAGDNSMIDAIAEKNSIANINRIYVGEQLTIPTSNDSSATTENKTTENTASTTET
                     ATQEHTYVAPVETVEVAPAAPAAATAPTSSSAKEWIAQKESGGSYTATNGRYIGRYQL
                     DASYLNGDYSAANQERVAEQYVTSRYGSWDAAKTFWLANGWY"
     gene            complement(12137..13288)
                     /gene="queG"
                     /locus_tag="IXT20_001494"
     CDS             complement(12137..13288)
                     /gene="queG"
                     /locus_tag="IXT20_001494"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HAQ4859946.1"
                     /translation="MTLKEEIIQESKRLGIDKIGFTTAEPFDSLKESLEEQKAAGHTS
                     GFEHPNIDERLYPEMTFDQPKSIISIALAYPTRIHEEVPRDEKRGQFARASWGIDYHD
                     ILRDRLNRLIEFIQTRAEKWQKEEEWRLAPQVDTGELIDVAVAQRAGLGFIGRNGLLI
                     TEEFGSFVYLGEIVTNIVFEPDKPGEFGCGDCMRCVTACPTKALLGDGRMNAQRCLSY
                     QTQTKGMMPEEYRKKMRNVIYGCDICQLVCPYNQGKDFHFHEEMEPKVDEIYPKLKPM
                     LSMSNKDFKQQFGHLAGSWRGKKPLQRNALIALANLGDRTALPEISECLTDVRPVIRG
                     TAAWAIGRLGTKEPEKWLDVLDEILAKETEEVVINELEHAQKLLRRKLK"
     gene            complement(13307..14059)
                     /locus_tag="IXT20_001495"
     CDS             complement(13307..14059)
                     /locus_tag="IXT20_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4859947.1"
                     /translation="MNFTRKDKYTIKEEVFRLISQQENTDKDQDLQFLEAELAKLFIA
                     NQNETTEPDEELYIGKWLFDEFQPLDENTGIGYFVFLSPVGFTPGVLVKQNDEITVKA
                     WPALFSVIKAEGVIKKIQSAYYKSQFIANSKLMNMFYNRLVEIARPGKIPQTFTYTQQ
                     KNLYEEVLYSRDLSEFSPWEEVRLHELLSTKPVSLNFFNALDNKRKLISTNYWEVFAA
                     RKKWMEEKKIADQQIQFVQLKKEKLYYGLYSR"
     gene            complement(14200..15150)
                     /locus_tag="IXT20_001496"
     CDS             complement(14200..15150)
                     /locus_tag="IXT20_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HAQ4859948.1"
                     /translation="MKTKKSLFLILAVSFLVLAGCGKQASDQADEGSKEKLSVVATNS
                     ILADMAKEVGTDQIDIHSIVPVGTDPHEYEVLPEDIKKASDADVILYNGLNLETGNSW
                     FDNLMETAKKEEGKDYFAVSKNVEPLYLTSGEEHTKADPHAWLDLSNGIKYVEEIARI
                     FSEKDAENATLYKKNAEAYVEKLKELDTQAKESFASIEENKKLLVTSEGAFKYFSRAY
                     DLPAAYIWEINTESQGTPDQMKAIIDQIRAKEVPVLFVETSVDSRSMERVAKETGLKI
                     YDKLFTDSIAKEGEQGDSYYQMMKWNIETIHEGLSQTKES"
     gene            complement(15151..15990)
                     /locus_tag="IXT20_001497"
     CDS             complement(15151..15990)
                     /locus_tag="IXT20_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HAQ4859949.1"
                     /translation="MIQSFIDGLMNYQFLQYALVTSMLVGLASGVIGSFIILRGMSLM
                     GDAISHAVLPGVAISYMFGFSYIFGATAFGMLTAAVIGFVTQRSRLKNDTAIGIVFSS
                     FFALGIILISYAQSATDLYHILFGNVLAVRESDLLLTALVSGIVLIFVFFFYKELKIT
                     SFDPTMAKAYGLNTSLIHYLLMFFLTLVAVVSLQTVGTILVIAMLITPAATAYLLTNH
                     LLKMIITAAGIGMLSAVVGVFFSYSYNWPSGATIVLACTAFFILAFLFSPTKGILFQK
                     GSR"
     gene            complement(15987..16733)
                     /locus_tag="IXT20_001498"
     CDS             complement(15987..16733)
                     /locus_tag="IXT20_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HAQ4859950.1"
                     /translation="MERTAIVIQDLSAAYQGKTVLKTINLTINPQKITGIIGPNGAGK
                     STFMKSLLELIPATTGKVTFSGQSVNSVRKKIAYVEQRSELDLSFPIDVLGVVLLGTY
                     PSLRIGQRPGKKEKERARQALKKVGMEEYAKRQISELSGGQLQRVFIARALAQGAEWI
                     FLDEPFVGIDALSERKIFDILQELKNSGKTILIVHHDLHKVEEYFDEVILLNKQLIAS
                     GPVQEVFTSKNLQLAYGEIIRHLVKGGEKK"
     gene            16971..18341
                     /locus_tag="IXT20_001499"
     CDS             16971..18341
                     /locus_tag="IXT20_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ4859951.1"
                     /translation="MEEEKLTRSLSARHIQMIALGGTIGVGLFMGASSTIRWTGPSVM
                     GAYAIAGFFLYLIMRALGEMLYVDPSTGSFANYASEYIHPVAGYLTAWSNIFQYIVVG
                     ISEVIAVGSYMNYWWPDLPAIIPGIIVVLFLMLANLISVKAFGELEFWFSIIKVITII
                     LMIIAGLGVILFGFGNHGQAVGISNLWKNGGFFTGGVKGFFFALSIVVASYQGIELIG
                     MTAGEAENPKKTIIEAVQSTIGRILIFYIGAIFIIVSIYPWNQLSEIGSPFVQTFSKV
                     GITFAAGLINFVVITAALSGCNSGIFSASRMVYTLAINGKMSKKFLKLSRHGAPFYPV
                     VAISGGILIGLILNYLLPMFINQSENIFVFVYSSSILPGMVPWVVILISEIRFKKKHA
                     DKMHDHPFKMPLSPYTNYVTLAFLAIVLIFMFINPETRISLIIGLVFLLYMTIHYFVR
                     EKRTGK"
     gene            complement(18396..19292)
                     /locus_tag="IXT20_001500"
     CDS             complement(18396..19292)
                     /locus_tag="IXT20_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAQ4859952.1"
                     /translation="MYDENEWGENVRMTRRRQQKQAARKRMIMIGAAVAVFLIIAGGV
                     AGLYFHGKNSSEAKSTTITETTTKKTETKQTSESSTKTKETQTSEEKTANGLPEQLQA
                     IVNDYSKKIEEKTPALIEEYQTEIQGNQEGIAGLSAVANQKARELQAISDEGIRKLRA
                     AYQAAENKDGVDLDTLINQLSANYTNHVAKISDIYLQTSASLQAESTSTQDTTSSDSE
                     TAESVEASEMQARTTQDTTDSSETNTNSATSQAATTVVLEGEGPNQIAERTGVPVETI
                     LSLNGMSMENYFLTPGETLKLN"
     gene            complement(19573..20250)
                     /locus_tag="IXT20_001501"
     CDS             complement(19573..20250)
                     /locus_tag="IXT20_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HAQ4859953.1"
                     /translation="MRTLIIISHPTIEESLNQQFFKEASAGLPATWHHLESSYPDGKI
                     DIETELQLLAQHDRIIFQFPFYWYSSPAHLKIWQDNVLERAAHVLKGKELGIVLTTGV
                     AEKEYQAGGKEEYTISEFLRPYQRIANKFHMTYLPPFVLAQFMYLSQEKRWEKLIAYQ
                     QYLSLEGKPSLTQRIDWFIQRVQENQKMQEEESEKQAYIVEALTEAKEQIEDLTFTLQ
                     EMKGTSL"
     gene            complement(20341..21246)
                     /locus_tag="IXT20_001502"
     CDS             complement(20341..21246)
                     /locus_tag="IXT20_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAQ4859954.1"
                     /translation="MEQSIFLKKEHQQVEKELYFDFCGFSKTMPFHSFGPAIRNDYIL
                     HVVMEGKGSYHVKNQQFQLKKGDLFLIRPGDSTFYLSDGEDPWMYCWLSFGGSAAEEI
                     IRQSLFREDQYTMVSSDIQKYIDIILECMKDTEETLENELQLTALTYRFLSVLLKDGG
                     KVSGGVQKVHSSLAVETVKYIEAHYAEKLSVEEIAQHLAVNRSHLSRVFKNHMGTSIK
                     EYLIGVRINRGAFLLSLTDESVESIAYQVGFNSLVVFSRMFKKSTGETATNYRKRMKN
                     ENFKNIALDTLKKQLEKQTAVSWTT"
     gene            21421..22875
                     /locus_tag="IXT20_001503"
     CDS             21421..22875
                     /locus_tag="IXT20_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ4859955.1"
                     /translation="MKSKMIQRVSYALGAFGHDTFYVTLSTYFMVFVTSQMFAGTDKA
                     TNAKMIATVTSLVVGIRLVEIIFDPLIGGIIDNTRTKYGKFKPWLVIGGVVSSVLLVA
                     IFTNFFGLATHNTTLFIILFAIVFILLDSFYSFKDIAFWSMIPALSTETKEREKFATF
                     ARFGSSLGANGTTLVVVPIVTFFTYLVTHKHIEGASGWFWFGVIAAVISGGTAIITAL
                     GTKEVDTAIRQQNEKAGIKDVFKAVAQNDQLMWLALAYIAYAIANAATTAVLFYFFKY
                     VLGQPTEFWIVGLVAALLGLVAVPLFPMLTRVISRRYVYLGGIAMMISSYFFFTIAGS
                     SLPIVIIGLVLFYFPQQLIFLSALMTITDSVEYGQWKNGIRNEAVTLSIRPLLDKIAG
                     ALSNGIVGFVAIASGMTGNATAADITAHGVTTFKAYAFYAPAALMIFSAIIFAWKITL
                     TEKKHAQIVEKLENKLAPADTMEDELHDAVDPSH"
     gene            22891..23922
                     /locus_tag="IXT20_001504"
     CDS             22891..23922
                     /locus_tag="IXT20_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HAQ4859956.1"
                     /translation="MKITEKDFGQGYHLITLENKNKLALSISDLGARIVSLKSNDREL
                     VLGFDTAEEYIEKDPYIGASIGRTAGRIENGRFSLNGKTYQLATDPKTGHNLHGGAPG
                     FELKKWSYVILNGENEASVIFTTTSPDGEHGFPGTMDVEIRYTLTKDNIWRVTSRGTS
                     DQDTLFNPTNHVYFNLTGDASQSIDQHELWLNSEAYAPLRTDSIPIGVKENAAGSAFD
                     FQIPKKLASVFASDLDQKNLVDGIDHPFFLKETGLGKEAARLTSPDKRIQVDIATDAS
                     SVVIFTANFGTETPEMRNRKLAHHGGITFETQTAPGAERFSAFGSIHLKAGSVFETVT
                     EFKIKTRKE"
     gene            23929..25116
                     /locus_tag="IXT20_001505"
     CDS             23929..25116
                     /locus_tag="IXT20_001505"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HAQ4859957.1"
                     /translation="MEIKRSLNTKFAETFGPKKTTQYFSPGRINLIGEHTDYNGGHVF
                     PASITYGTQGVAAPRDDNKILVYSTNFDDIGVISFTLDELEYDKKANWANYVKGMVLK
                     LKEAGHSIDHGFELLVEGTIPNGAGLSSSASLELLVGVVLEDLFDLKTDRLELVQTGK
                     KVENEFIGVNSGIMDQFAIGFGELDHAILLDTNTLKYEMVPVKLDGYAIVIMNTNKRR
                     ELADSKYNERRSECEEALARLQKTLPISSLGDLDEEEFESTIDQIGDDTLIRRARHAV
                     SENQRTLKAKAELEAGNLEAFGQLLNDSHHSLRYDYEVTGIELDTLVDAAQKQEGVLG
                     ARMTGAGFGGCAIALVKEENIPAFKNNVYDEYMKVVGYAPEFYVAHIGNGTTKIDPEA
                     VHV"
     gene            25272..26654
                     /locus_tag="IXT20_001506"
     CDS             25272..26654
                     /locus_tag="IXT20_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HAQ4859958.1"
                     /translation="MWANQNPVFVYEKEPSRDILCIDCKSFYASVECVEKDLDPLTTK
                     LVVMSYPSDSTETRGSGLILASSPAAKKAYGITNISRARDLPFPYPSDLYIVAPRMAY
                     YMEKNREINSIYKKYADEANHHVYSVDESFLDVTNSMALFGKTNAYDMAKLIHKDVFE
                     QTGIYTTIGIGDNPLLAKLALDNGSKDGKQMFAEWRYEDVPEKLWKISDITDFWGIGK
                     RTAKRLKQMGIHSVYDLAHADYYHLKSKLGVIGTQLYAHAWGIDRSFLGQEYTPKSKS
                     IGNSQILDRDYTRREEIEIVIKEMADQVGTRLRRSAAKTQVVSLWVGYSLGYLDPNGK
                     TGFHQQMKIEATNASRDLADALLTIFHQHYHYQDIRRVGVNCSDLVYATGLQLNLFED
                     PEKQINEAKVDFVVDTIRKKYGFQSIIHAHSLLQGGRAIARSSLVGGHAGGMSGLEGG
                     KDETDSKKIH"
     gene            26781..27011
                     /locus_tag="IXT20_001507"
     CDS             26781..27011
                     /locus_tag="IXT20_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4859959.1"
                     /translation="MTLQEIDSILTEAFLSRKAVIIQLNLKDEFGRLLDEVEGHFIGE
                     AYEDYFMIDHQPILWEDVRYIELKETKKWFVT"
     gene            complement(27211..27879)
                     /locus_tag="IXT20_001508"
     CDS             complement(27211..27879)
                     /locus_tag="IXT20_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HAQ4859960.1"
                     /translation="MIFSITMKLVFGLIGLLLVVRLLGKKSMSEITPFDLVYTLVLGG
                     ILEESTYDDNVHVGHVLFAIALWAVMIYGIERIVQKNEKVNRWVKGEPSVLIKDGVIN
                     MTELTNNHIEMEQLRAILRQQECFFLENAKHVILENAGQMSVLKKSDEDKALSILLVD
                     EGQIQHKVLQSNQLTEAWLMENLKKEGYADVKQLIYVEWSEEKGLYILNKEDVMHESY
                     RIDG"
BASE COUNT         7829 a         5750 c         5159 g         9366 t
ORIGIN      
        1 agtagcttct tcgaaaaata agccgaaatt tcacaaaaat ttgagaagca attttcgtaa
       61 attccttctt atttcttgaa gctgaccact tctgtcacaa cctctaatta ttaaagaacc
      121 ttttgcgaag ttaacaatct ttctttgatc aattgaaaat tctgcctaag tagtgataga
      181 tcctgtgtga ctttcaacaa taagatactt ccctggattt cagcaatcaa cgctttggct
      241 tgacggtctg cttcttcatc ggacagtttt tcttcagaaa gtgccaggat attttgtttc
      301 gtatacttga cccattgatt gactaattct agtagcaaca gacggtattc ttcatccact
      361 gtactcaact caataatcaa attacctaca ggacagccat gataagtgat ctgtgtctca
      421 tgatcagcga aaatccagtc caacatttct tctaatgctt cttctggctg ttttccagca
      481 tttaagatgt cttcgaacaa ttctgtctgc catacgtcca tatgcgcatg gatcaccgct
      541 agacaaactt ctttttttga agaaaagtaa taataaaatt gaccttttcc tgtatcagaa
      601 gctgctagaa tatctcggat tgacgtttca tgatagcctt ttttataaat aagatctttg
      661 gtcgcttcca agatcttttc ctttgttttc aatgatattc tcctttttca tttctcatag
      721 tcctatatta tacagtaaat tcatcatttt ttcttcttaa tcgtttaaaa aagactgtgc
      781 caaattatct gacacagccc tcactataaa actgttctca tatagctaga tgttcattga
      841 ttcaagaact gcgaggacaa aagcaattcc tatcttatga tagttgctct aatacttcct
      901 ctgttgtcat cacttgatct gcaataaagc caaagttcgt caacgctgca tccattgctt
      961 cttggccagg agcaccaatt gcatctgtcg ctaccacaac attgaatcca ctctctacca
     1021 attcacgcat atgggaatcg ctacacaagt ttccgttcat tccaccaaga attactgtgt
     1081 cgattccatt ttttctcaat tggagcgcca aatcattgct ttctggtcca aagattttat
     1141 gaggagagct gatgatcgta ttttcatcca aataaggttt caattcttcg ataaaatcgg
     1201 ctcctgaacc ttcttccact gattcatatt gactttttct agcaaacatc tgctgctcaa
     1261 gcatcatatg ttctcctgca ccgccaaatt gccatttatg gtcatgtggg taaaaataat
     1321 gaggagaaat aaagagtttg tatccttttt cttttgcttg ttttaccatt tcaatcatat
     1381 tttcgatcgt attgtatttt tctaagatat cttttgtcaa accaaagcca ccgcctgctg
     1441 gtttcaagaa ctcgacttgc gggtcagtga gaactactgc tgtattttta ttattgaaca
     1501 tggtggattc tccattcgtt atttatttta atacatcatt tagagcttca ctcattgtcg
     1561 gatgagaata gatttgatct ctcaataacg tataaggaag ttttccattc atagctagcg
     1621 aaatcaagtt gatgacttca tgagaatctt ccgcatagat acttgcccct agaatcaagt
     1681 cagtttcagg gtcgactaaa actttgaata cacctttagg atcttctaat acttgtgctt
     1741 tagggacacc gcttgtcata tacttgaata atttgtaatt gacgccttgt gctttggctt
     1801 gtttttctgt taaacctaca ttggataaag tcggagtgat aaatacactg taaggcacag
     1861 ctttccggtt attcgttgtt cgtttattct cgcccttcaa ctgatccata atgattcgat
     1921 aatcatctag agagatataa gtgaactgca atccgccttt gacgtctcca atcgcccaaa
     1981 tgttgtctgc atctgtacga aggtattcat ccacagcgat tgctccgcga tctgttagtt
     2041 taacatctgt attttctaat ccaagtgatg ctgtacttgg ttttcttcca gttgcagcca
     2101 aaacttttga cgcttggatt gttacttctt ggccatcttc tgtaaaggtg acggcagctg
     2161 ttgattcttg gtctgctact tgatctacag atacacctag atggaattcg attcccgcat
     2221 ttgtcatgtc ttctaaaatc atttgcgcga tatcttcatc ttcacgtgga agaaattcag
     2281 aatgggcatc aagaacgaca actttagatc cgtattcatt gaacattgat gcaaattcca
     2341 agccaatata tccagccccg atgatcacta atttttccgg taattgttcc tgatccatcg
     2401 cttgcgtaga atcaatcaag tagcgacttg tattcaatcc tttgattggt aaaatgattg
     2461 gttcagaacc agtattgatg aaaatccgtt cgccttcaac aatcactttt tcaccattat
     2521 tcgtcacttc gatttgatgg ttagaaagaa aacgtgctgt accgtccatc actgtcccag
     2581 ttgcctcatc gctgatcata tgataatttt tggcgcgaag catgcccgtc aatttttctt
     2641 tccgagcaac agcctctgta aagtcgattc ctctttctcc attgaaaatc aatgatttag
     2701 aaggaataca gccgatgttg atacatgttc cgccatacat acgtgttgac ttttcaacga
     2761 ctaaaaccga ttcacctttt gaagctaacg ccttcgctaa agtcttaccg ccctttccaa
     2821 aaccgatcac aatattttga tatttttcca tcatttttcc ctcgcttctt ttttacctac
     2881 cgattggtac aaaaatatga taaccgataa ttgtctattc tccaaatcat ctgctttatt
     2941 tgtcaaaaaa aaccagacaa aaagcggtcc tttcacgttt ttagtctggt tcgatttcct
     3001 ttattcttct aaacaatcgt tacttctaca tcgatatttc ctctagttgc tttggagtaa
     3061 gggcagaatt catgcgcatc ttctgcaatt ttttgggctt cttcttcgct gatgccatcg
     3121 atatgcactt ctaatactac accgatctga tagccgccat catctaaatt ataaagagag
     3181 acacgtgctt ttactgtgga ctctgctgtc attcctgctt ctttgatcat atgttgtacc
     3241 gccccattga aacaagaagc ataacctgct gcaaataatt gttctggatt tgttccttct
     3301 gcatggattc ctggaggagt gattttcata tgcatcgagc catttggtgc ctcgacttct
     3361 ccattgcgcc cgccatggtt gatcattgct gtttcgtatg cttttttcat tgtactgctc
     3421 ctttcgattg accatttgct acttggctat aattatagtg tatgacaagt aaggcgcttt
     3481 ctacaaaaat tctgcttatt cgaataccag aatgccgtct tctactattt ttccaaaaaa
     3541 caaaaaaata ctactcgtga acaacagcgt catatttgtt ccaaaataaa tagcaatctt
     3601 ttctacataa tcttttatat gattgatttt aagatacata tataccagaa agttgacccc
     3661 ataaaccaat actgttaaaa aaataaccat attcgttacc attttttcct ccttttgctg
     3721 gtctccctac cagcactcga tttaaccata ccactttttt tgccgtccaa gcaactagcc
     3781 tttcaaaaag actggtttat tctgctgtta atgattcaag atcagaaaaa tgtccgaata
     3841 attttgctaa ttttactaac gccaacttgt tctttccgaa ttcatcattt tctatctgta
     3901 atactagtaa cggctcatgt aagcttaaac ggatcaaaaa ccagccgttt ccgtatggct
     3961 cttctagatc atatcgaata ccttcttcat tttccgggtc gaagttcatc cctttttctt
     4021 cttccaacag tcgcttcatt tcttctaata tcttgtttcc ttgttcgcgt ggttcatcgc
     4081 tgaacaattt taatcggatt tcttgtactt ctacgggctg tttcaactct ttgatttgtt
     4141 catctaatgt gatacctttt ctttttaatt ctggcaaaag catcaggatc tttgcgatga
     4201 catatgcacc atcatccaga aaatagtttt ccttgaatgc agcgtgtccg cttgtttcga
     4261 tggctagctg tgtatctttc ccaagacggt taagttctaa tgctttattg attacatttc
     4321 gatagccgga aatataacga acttggatgc ctcctttttc ttcaatgaat ctttgtagat
     4381 gagaagaagt aggggaattc gtcacgattc ttgttcctgg atgctctttt aatacgattg
     4441 tacttaatac tgcgatcaaa ttatttcgat tgatcactgt tccagaagca ctgacaactg
     4501 cagaacgatc cacatctgta tcgaaaatga tcccaagatc tgcttcattt gctaaaacag
     4561 cctgcttgat actttccatc gcttctttat tatcaggatt tgggatatga ttaggaaatc
     4621 ggccatctgg ttccaaaaat tgggaacctg ttgaatccgc acctaagcta ttcaatactt
     4681 tttcagcaaa gaatccacca gcaccatttc cggcatcaac aatgattttc caaccattta
     4741 gtggtttgct ttctagtgtt ccaatcccct ttttaatttt tgtgactaaa tcttgcgcat
     4801 aaggtgtcaa aaggtctgct ttttctacgc ttccagtttg ttcgcttttt attttttgct
     4861 gctgtgatag gataaaagca atatcttctt tttctgctcc tccttctgag gtaaagaatt
     4921 ttattccatt ataataatag ggaagatggc tagcagtcag cataaccgca gcatcgcacg
     4981 agaactgttc aaattgagtg gacataaaca tagcaggcgt agtagctaat tcgaaatcga
     5041 tgacatgtat cccttgttct ttcagacttt ggatcaacgc ctctttcagt gctggtccgc
     5101 ttatcctact gtcatgacca atcccaatcg tccatttttt atttggatcg gttgatttct
     5161 tctttttctt tatccagttt gctagtcccc agccgatctt ttttaccatg tctgtcgtca
     5221 agtttacttc atatccttca actggtaatg cgattcctca aatatccgat ccattttgca
     5281 gcgctttcat atctttcata taaaagtcct cctcatttat tccctcattt tttccagctt
     5341 ttctgcaaat cgctgtgctt cttcttctga gcggcaaata ataatgaatg tatctacacc
     5401 ggccatcgtt ccgacaactt cagggtaagc cacttcatct agccaattcg ttaccacatc
     5461 ggcaccattt cgttcagtca atacgataac catgaactga acagactgga tacgcaaaac
     5521 ctcgcgtcta attgctgtac gcaaacgttc ttcatttaaa gaaacagatg gctggttaaa
     5581 taatgtatag cgtactattt tgttttcatt ttgacttttt actaacttca tttctcgaat
     5641 gtctcgagaa atcgttgctt gagtcacatc ggcgcctgct tcatgcagcc gcgcaagcag
     5701 ctcttcctgt gtcccaatag gttgttctgt aatgatctgg cggatcagtg cttgtcgctg
     5761 tttctttttc atattagaca cctctcaatg ttgattatag caaaagataa gtaatatttc
     5821 ttcatatttt ggtatttttt acttttattt tttgttttca ttcaggttgc taaccttttt
     5881 ctattttaac acgcaaaatc gtcttcattt tttcaggtgc tgattttctt ggatcgctga
     5941 tgtaaatctc tttgtgttct ttagaagctc tttcatagcc ttcttttgcg atgaatgctt
     6001 ctaatttctc aaacgttgct ggttcttcgg catagggccc gacgtgtaag atttgggcac
     6061 aaagaccttc ttccatcttt ttgaatacta cttgttcttt caagtcttcc ggaagtttgc
     6121 tagaacgctg aatggcttct tgtgctatcg cttctgtaac aaagtcaggc tgtcggatca
     6181 tcaattgata agaaaaatgt tcttttttca tcactggttc atgcaaatac ttttcttgca
     6241 gtccccattg tccttccagt ggatagacag tatacatctg ataatcagga atcagccaat
     6301 tttttttagg cgacatgcgt attgcatagc tgactgcata tagacaggct actcttcgtt
     6361 taaagtcttc ctgattcggg tcgccgatcc ctctgatgct aaagaatgat tttttaggaa
     6421 cctctagtat ttgcgggctt tttttcatac tatagctctt gtctttacgc cattcaattt
     6481 tttccatttt tcttcctccg cgaaattcca tttagttatt tattatcata cctctttttg
     6541 agtgacttgg ctaatcgtgt tgacactatt tttcagtttg tgctagaata agtgaagtaa
     6601 ataaatcgta cagataacac gtttcggcgt gtcgagagag aggcggtttc tatttttata
     6661 atagaaatca ctctcttttt ttacgaaatt ttttagaggt gatgtaaatg acaaaacaaa
     6721 aacagctgct tttagtacct gcagtaattg caacagggat gatgtctttt gcgggcgtat
     6781 tgatcgagac ggccatgaat gtaactttcc caactcttat cagacagttt gatttaacga
     6841 ctgcacaagt ccaatgggta acaacgattt atcttttgat gatttcgatc attgttccgc
     6901 tgtctactta tttgaatcgc aattttagtc tcaaaaaact ctttttagct tcaaatctgc
     6961 ttttcttggc aggtataatg atcgatttct tctctcctac cttcatggtc ctattgtttg
     7021 gtcgattgct ccaaggtgca gcgacaggta tcgctcttcc gttgatgttc catattatct
     7081 tgacatttgc ccccctcaat cgacgaggcg cgatgatggg tgtcggtact ttgacaacgg
     7141 ccattgctcc agcaattggc cccacttatg gaggcattat gacttcacat tttacatgga
     7201 atcatatctt cctgtttctt gtgcccgttt tgtttctttc tttgattatc ggctggtttg
     7261 ctattcccga gatggcagta caaaaaagtg gttatttgga ttggataagc gttttaggaa
     7321 tttgtctttt gtttagtgga agtctaacat ttttgagtac gttgggaaca gtttccagct
     7381 ggattgcttt aatcatcggc ggtattggct ggttcctttt ttactatcga agtaaaaaag
     7441 tggagcatcc actgatccga ctgtctattc tttcaaataa aacattccgc ttgtttttga
     7501 ttggcttctt agtttatcag tttttattac ttggtgtatc ttttgtcttg ccgaatttcc
     7561 ttcagatcgt tcaaggaagt agtccttttg tcgctggatt agcgatgctt cccggcgcag
     7621 cggttggtgc tttgctttct cctttttccg ggaaaatgct ggatcaatat ggtccgaaga
     7681 agccaatttt tgctgggctg attctttctt tgattggttg gctagcatta ttcctactca
     7741 taggacacgc ttcagtgggt gtattgattg cttgtcatgt gttttatatg atcggtatcg
     7801 gattatctta cagcaatatg atgaccaccg gaatgaatgc actgaatgaa gaactacaag
     7861 gagacgggaa cgcaatgttc aataccttgc agcagttttc tggcgctgtg gctacttcat
     7921 tagtagcggt cattatcaat tacgtccagc atcatacttc ccataactat gaagtgtcta
     7981 ctacattagg atcaaaagca gcattaggcg tcttgttgct gttgcttctc gtaagctttg
     8041 ctcgattcgc ctattatctg ttctttgcta aaaaagcgta gattccatat acggcaggtg
     8101 ttgcatctgc cgtttttttg tgtgaaaatg gttgtttgta aaaggaggct tttttatgga
     8161 aaaaattgcc attatcggtg gcgggatcat tggtatgacc ctagccagtt acttagatac
     8221 atcaaaattc aatgttgctt tatttgatga agggacagga caggcaacta aagctagcgc
     8281 tgggattatc tccccttggt tgtcgaaacg cagaaacaaa caatggtatc gtctggctaa
     8341 agacggcgct gcttttttcc ctaaacttat aaaagatttc caacttgatc aaacgattta
     8401 cgcacaatca gggacacttt tgcttcgacc tgaaaaagat ttagctgaat tggctgttct
     8461 tgccgagaaa cgaaaagaag aagcgccaga aattggaaac atcgaatggc tttcacctga
     8521 acagacatct caagcacttc cgttattgaa accagcagtt tcactgcgta tcagtggtgg
     8581 tggtcgtctg gatggaaaag catatttaga acatcttggt cagcgtttag cacaaaaaca
     8641 aatggagcag atccaggcaa aagtcacttt gcataagaag agcgattctt ggctcatcaa
     8701 gggaccgttt ggagaataca cagctgatcg tgtaattttg acacctggcc cggcacttaa
     8761 gcctttatta caagaattag gctatgctgc agatatacgt ccgcaaaaag ggcagctctt
     8821 agcatttcag acatcttata agatgagtca agactggcct gttgctatgc ttgacggaga
     8881 agctgatttc attcctttca tggatggaaa gatcctttta ggtgctaccc atgaaaacga
     8941 tcaaggctgg gatttggctc ctactagcga agcttataag ggattgacta agagtacaga
     9001 acagttcttg actgatccag atcagataaa aaactggcca tacgagttcc gtgtaggtac
     9061 ccgtgcttat acgtcagatt tttccccatt tttcggcgta ctgccagatg atccaagctt
     9121 actggctgct agcggtctag gctcttctgg cttgaccaca ggtccttata tcggctatct
     9181 attagccaac ttcttaaata ctggtcattg gcctacggaa gattatcaaa aggacatctc
     9241 cacctatatt acgaaaaatc catcatagaa aaacagcttt ttcaagtaag gatcccgagc
     9301 gatattgcgc atggattact tcttgaaaaa gctgttattt tagttcaatt tctgaatcaa
     9361 attctccaaa ctttcttttg cttcatctgg caagagatct tttcctatct gagcttcaat
     9421 gaatcgtaaa cgaatatcaa gtcgttgttt caccagattt ttataggacg tatcctcaaa
     9481 agaaacagga aaatctggta taggtaaata gtggatgtct tcaaatggac caaacgactt
     9541 gaattctgcc gtttgttgca cgattctttc aacgattgac aatgtcgttt caggtttgat
     9601 ttttttatca tcgaaatatc cagtattcag gacatagcag gctgtttgct gtttcaaaaa
     9661 caactctcga aaagcttgat agtcttccct aagaggatag acacggaaag gattagcaaa
     9721 tggttcaatg actagctcat ctagctgtct ttttgtagtg atattttccg cgtctgatcg
     9781 ttttgttgct aatgttgcac caaaagacgc tgctaaaact gggtcattga tcttgataat
     9841 tggcggaagc gaatcatctt tcatgatcca aaaaacagca tcgatctttt cgctaagatg
     9901 atcgactcgg tcgggtgtcg cataacgtga tttgactgtc cgcccatttc cgtttcgtat
     9961 atctcctgtt actaggactt tttctccttt acttgtacat gtcacgccca cattctgaca
    10021 agtaaggaaa gaagaaatgg ccggatcatc agacggatag tcttgtgttt tatcaaagta
    10081 agaaggttcc aaggcagtag caaaaccatt ttttcgagaa ataacgaaag catcgtcatg
    10141 caagatcgaa acagagtgac cttccgtatg ttttgctaag gtaatcgttg attttccaga
    10201 accggacaac ccaaatacag ccatcgtata ggtctggtca ttactagcat attgtttcat
    10261 cccaccgtgg caggcaataa agccattgcg atttcccaat gcccaagcaa gcgtcaaagt
    10321 cgcttttttt aattctccaa agtaacgtaa tcctagcaca gccgctgtat tatggagagc
    10381 atcaaacaaa atgagtccat ttggaaatgt ttcatgtgtc cattcgggat cacaaaagat
    10441 ataaagatct ccctcatcaa aggaaacaga cttttgataa gtctctaaga tcttcgaatt
    10501 cgctggctga aaattcagca aataagaata caaattgttc tcaaatcctt ctggaatcag
    10561 caaatgactt tgcacagaaa attcttcaga taggccaacg attacttttg tatggtaaaa
    10621 ctggcgcttg cgactttgat acacagcttc tcttaaaatt ttctcgtatt tgctttgttc
    10681 cttgtctaag tcctcaaaga tccgccgagc acttgctgtt cttcccacaa tgcttccatc
    10741 attgaatact agtgtcctaa catcagctgg taaatctagt tcttcagcat gtgagattgt
    10801 taaatctgtg atgatcgttc ctggactccc ttttgctagt tgataagctg tcttcctatc
    10861 aaaaacttca tgaacattat taccgtaaaa tgtcgtttct acaatcgatc gaatactgga
    10921 taacagtgga tttccacttg tgatttcgtt taattcgaga ctctcttttg tactcatggc
    10981 tcctcctcat tccctaaaca aaaaaacgtt ttctttatta tacattattc gagtttttat
    11041 ttgtactgct gcgctgtggg aagcaaacga gttatttttc acacaaaaaa accgagaact
    11101 tgaataaaca agtttctcgg tttcatcatt ttccttttat cggcttagta ccagccgttt
    11161 gctaaccaga atgttttagc agcatcccat gaaccataac gagatgttac atattgttct
    11221 gctacacgtt cttgattggc agctgaatag tcgccgttta agtatgaagc atctaattga
    11281 taacggccga tgtaacgtcc gttagtagct gtgtatgaac cgcctgattc tttttgtgcg
    11341 atccattctt ttgcagagct tgaagttggc gctgttgcag cagctggggc agcaggagct
    11401 acttcaactg tttcaacagg tgcaacgtat gtatgttctt gcgttgctgt ttcagttgta
    11461 gatgctgtat tttctgttgt tttgttttct gttgttgctg aagaatcatt tgaagttggg
    11521 attgttaatt gttcaccaac ataaatgcgg ttgatgtttg cgatcgaatt tttttcagca
    11581 attgcatcga tcattgagtt gtctcctgca aatttttgtg agatcttaga taatgaatca
    11641 cctgatttta ctgtataaac ttcatcagcg tgagcatttg tagttcctac aaacattgca
    11701 gtagcaccag cagcaaaagt tgtacctaaa acaattttct taaatgaatt catattaaac
    11761 tccttttttt ctccgttttt tattctttcg acgctattaa ctttatcatt tagaaatatt
    11821 tcataggtaa aagaaacatg acatttaatg acaaaataac gaattattta atatttgtaa
    11881 catttaaaca aatatttttt tgatacaaaa ccataaaagc cttgttgtat aagcaaagta
    11941 atcgtttttc tatgtttttt tatttttgaa tgaaaaacgc agattctgta acattcactg
    12001 caataatcca taaaaaaaag caaaaaaatt tttgattttt atcaaattct cttaaaaaaa
    12061 tgtaaaaaat acaaaaaaac cagtaattgt tacaacaatt actggacaaa aactttgaat
    12121 aatgttaact atttttttat tttaactttc tccgcaataa tttctgggca tgttccaatt
    12181 cgtttataac gacttcttct gtttctttag ctaatatttc atctaataca tcaagccatt
    12241 tttccggttc ttttgttcct agacgtccaa tagcccaagc tgctgttcct ctgatgacag
    12301 ggcggacatc tgttaaacat tctgaaatct caggcaatgc cgttcgatca cctagattag
    12361 ctaaagcaat caatgcgttt ctctgcaaag gtttcttccc acgccatgaa ccagctaaat
    12421 gcccaaattg ctgtttgaag tctttattag acatcgaaag cattggcttt aattttggat
    12481 atatttcgtc tacttttggt tccatctctt catgaaaatg aaaatctttt ccttgattgt
    12541 aagggcagac taactggcag atatcacatc cataaatgac atttcgcatt tttttacgat
    12601 attcttccgg catcatccct tttgtctgag tttgataaga caaacatcgc tgggcattca
    12661 ttcttccgtc accaagcaat gcttttgtcg gacaagcagt cacgcagcgc atacagtcac
    12721 cgcaaccaaa ttctcctggt ttatcaggct caaatacaat attagtaacg atctcaccaa
    12781 gatagacaaa tgaaccgaac tcttcagtga tcaacaatcc gtttcgtcca ataaatccta
    12841 aaccagctcg ctgtgctaca gcgacatcga tcagttctcc agtatctact tgcggagcta
    12901 gacgccattc ctcttctttt tgccattttt ctgctcttgt ttggatgaat tcgatcaatc
    12961 ggttcaaacg gtctctgagt atatcatggt aatcgatccc ccaagaagca cgagcaaact
    13021 gcccgcgttt ttcatcccta ggcacttctt catggatcct agtaggatac gcaagtgcaa
    13081 tggaaatgat cgatttcggc tgatcaaatg tcatttctgg ataaagacgc tcatcgatat
    13141 ttggatgttc aaagccagaa gtatggccag ctgctttttg ttcttccaac gattctttta
    13201 atgaatcaaa aggttccgcc gtggtgaacc cgattttgtc aattcctaat cgtttgcttt
    13261 cttgtatgat ttcttctttt aatgtcatgg tcttcctcgc ttgctgttaa cggctgtata
    13321 acccataata tagtttttct tttttcagct ggacaaactg gatctgctga tcggcaatct
    13381 tcttttcttc catccatttt tttcttgcag caaatacttc ccaataattt gttgaaatta
    13441 attttctttt gttatctaat gcattaaaaa aattcaatga aactggtttt gtacttaaca
    13501 gttcatggag ccttacctct tcccatggcg aaaactcaga cagatcgcgg gaatacaata
    13561 cttcttcata gagatttttt tgctgtgtgt acgtgaatgt ttgcgggatt ttcccaggcc
    13621 tagcaatttc tactagacga ttataaaaca tattcattaa tttgctgttg gcaataaatt
    13681 gacttttata ataggccgac tggatctttt tgattactcc ttctgctttg atgactgaaa
    13741 ataatgcagg ccatgctttg acggttattt cgtcgttttg tttaactaat acgccaggtg
    13801 tgaagccgac tggagaaagg aacacaaaat aaccgatacc ggtattttca tctaaaggct
    13861 gaaattcatc aaacaaccat ttgcctatgt acagctcttc atctggttct gttgtctcat
    13921 tttgatttgc tatgaataat ttagctaatt ctgcttcaag aaattgaagg tcttgatcct
    13981 tgtcggtatt ttcctgctgg ctgatcaatc gaaagacttc ttctttaatc gtatatttat
    14041 ctttacgtgt aaaattcata gccacacctt ccttccttac tctaactata acggaatttc
    14101 ttcaaaaaag gtatctgtac gacgaaaaac agcataattt tttttcatat ctcttataaa
    14161 aaatgcaagg tctgctcctc aagctagacc ttgcactttt cagctttctt ttgtttgtga
    14221 caaaccttca tggatcgttt caatattcca tttcatcatc tgatagtaag aatctccttg
    14281 ttctccttct tttgctatgg aatcagtgaa cagtttatca tagattttca aacccgtttc
    14341 ttttgctacc cgttccatgc ttcttgagtc tacactggtt tccacgaata aaaccggtac
    14401 ttcttttgct cttatctgat caatgatcgc tttcatttga tcaggcgtac cttgactttc
    14461 tgtattgatc tcccatatat aagctgctgg caaatcatat gctcgtgaaa aatacttgaa
    14521 tgccccttca cttgttacta ataatttttt gttctcttcg atagaagcaa aactttcctt
    14581 cgcttgtgta tctaattctt ttagtttttc cacatatgct tctgcatttt ttttatagag
    14641 tgtcgcattt tctgcatctt tttcagagaa tatacgtgcg atttcctcca catattttat
    14701 tccgttagat aggtctagcc aagcatgggg atctgctttt gtatgttctt caccgctagt
    14761 taaataaaga ggttctacat ttttgctaac tgcaaaataa tcttttcctt cttctttttt
    14821 agccgtttcc atcaagttat cgaaccagct gttacctgtt tcaagattca aaccgttgta
    14881 taaaataaca tctgcatcgc ttgccttttt gatgtcttct ggtagtactt catattcatg
    14941 cggatctgtt ccgactggta cgatactgtg gatatctatt tgatctgttc cgacctcttt
    15001 tgccatgtcc gccaagatcg aattggtagc cacgactgac aacttttctt tacttccttc
    15061 atccgcttgg tcagaagcct gctttccgca accggctaat accaagaaac taactgccaa
    15121 gattaaaaag agtgattttt ttgttttcat ctatctgctt cctttctgaa ataaaattcc
    15181 ttttgttgga gaaaataaaa aagcaaggat aaaaaatgcg gtacatgcta acacgatcgt
    15241 agctcctgat ggccaattgt aactataact gaaaaacaca ccgacaactg cacttagcat
    15301 accgattcct gcagctgtaa tgatcatttt cagtaaatgg ttcgttagca agtaagccgt
    15361 ggcggctggt gtgatcaaca tggcaatcac caagattgtt cctactgtct gtaaactgac
    15421 tacagccact aatgtcaaaa agaacatcaa aagataatgg atcagcgatg tgttcagccc
    15481 ataagctttt gccattgtcg gatcgaatga tgtgatcttc aattctttat aaaagaaaaa
    15541 gacaaatatc aagacgattc cactaacgag tgctgtcagc aacaaatcgc tttccctaac
    15601 agctaagaca ttcccaaata aaatatgata caaatctgtc gcactctggg catatgaaat
    15661 caagataatc cctaatgcaa aaaaggagct aaagacaatt ccaatcgctg tatcattttt
    15721 caatcggctc cgctgagtca cgaaaccaat cactgctgct gttagcatcc caaaagcagt
    15781 ggctccaaag atgtaactaa aaccaaacat ataagaaatg gctacacctg gaagaactgc
    15841 gtgagagata gcgtctccca tcaaagacat ccctcgtaaa ataataaaag aaccgatgac
    15901 tccagaagca agtcctacaa gcatggaagt gaccaaagcg tattgcagga attgataatt
    15961 cattaatcca tcaataaagg attggatcat tttttctctc ctccttttac taaatgtctg
    16021 atgatctcac cataggccaa ttgaaggttc ttcgatgtaa agacttcttg tactggaccg
    16081 gaagcgatca gctgtttatt taaaagaata acctcatcga aatattcttc tactttatga
    16141 agatcatgat ggacgatcaa aatcgttttt cctgaattct tcaattcctg caagatgtca
    16201 aagatctttc gttcacttaa cgcatcaatc cctacgaatg gttcatctaa aaagatccat
    16261 tctgctcctt gggctagagc tcttgcaata aaaactctct gaagctgtcc acccgatagt
    16321 tcgctgatct gtcttttggc atattcttcc atccctactt ttttcaaagc ttgtcttgca
    16381 cgttcttttt cttttttccc aggtctttgt ccaattcgta aagatggata tgttcctaaa
    16441 agtacaacgc ctaatacatc aattggaaag gaaagatcca attcacttcg ctgttccacg
    16501 taggctattt ttttacggac tgagtttacc gactgtccag aaaaggtgac tttaccagta
    16561 gtagctggta tcaactctag caaagatttc ataaatgttg attttcctgc accgtttggt
    16621 ccgataatac cggtaatttt ttgtggattt atcgtcaagt tgatagtttt caaaacagtt
    16681 ttcccttgat aagcggctga aaggtcttga attacaatgg ccgtacgttc caaatcttac
    16741 actccttttt aggtttacct aactttttat ttaaatatag tttaactaaa atggaaagca
    16801 ttttcaagta gaatgatgaa aaagtttttt tcgaattttc ctcgcatttt atgaaaattt
    16861 tctgtagaat cacatcaaga gttcaataag gctaacctaa taatgaaaaa catgtgttga
    16921 aacgtttgat tattttttct tgttgactca acaacgaaag gagtttttgt atggaagaag
    16981 aaaagttaac tcgaagtctt agtgcccgtc atatccaaat gatcgcacta ggtggaacga
    17041 tcggtgtcgg tctgtttatg ggtgcctcct caaccatacg ttggacaggg ccctcagtga
    17101 tgggagccta tgcgattgca ggattctttt tgtatttgat tatgcgtgct ttaggcgaaa
    17161 tgctttacgt tgatccttcg actggttctt ttgcaaatta cgcgagcgag tacatccatc
    17221 ctgtggctgg ctacttgacc gcttggagta atatttttca atatattgtc gtaggaataa
    17281 gtgaagtgat tgctgtagga tcttatatga attattggtg gcctgatttg ccggcaatta
    17341 ttcccggaat cattgtcgtc ttgtttctta tgctggcaaa tcttatttct gtcaaggcgt
    17401 ttggcgaatt agaattttgg ttttcgatca tcaaagtcat aacgatcatt ttgatgatca
    17461 ttgcaggtct tggggtaatc ttatttggat ttggtaatca tggtcaggca gtagggattt
    17521 caaatctttg gaaaaatggt ggattcttca ctggaggcgt caaaggattt ttctttgcat
    17581 tatcgatcgt tgttgcttcc tatcaaggaa tcgaactgat cggaatgact gctggtgaag
    17641 cagagaatcc taaaaagacg atcattgaag ctgtccagtc cactatcggt cgaatcttga
    17701 ttttttatat tggtgctatt tttatcattg tgagtatcta tccgtggaat caattgagtg
    17761 agattggttc accttttgta caaacttttt ctaaagtcgg cattacattt gctgcaggac
    17821 tgattaattt tgtcgtgatt acagcggcat tatcaggttg caattcaggg attttcagcg
    17881 caagtcgtat ggtctataca cttgccataa atggaaagat gtctaaaaaa tttctgaaac
    17941 tatctcgtca cggtgcgcct ttttatcctg tcgtagcaat ctcaggtgga atcttaattg
    18001 gtctgatctt aaattatctt ctaccaatgt tcatcaacca gtcagaaaat attttcgttt
    18061 tcgtatacag ctcaagtatt ttgcctggta tggttccttg ggtagtgatt ttgatcagcg
    18121 agattcgctt caaaaagaag cacgcagata aaatgcatga tcatcctttt aaaatgccac
    18181 tatctcctta tacgaattat gtgacacttg cttttttagc tatcgtattg attttcatgt
    18241 ttatcaatcc tgaaacgaga atctcattga ttattggact cgttttcttg ctttacatga
    18301 cgatccatta ctttgtacgc gaaaaaagaa ccggaaaata aaaaaagaac gaagcttcac
    18361 cttagtggaa gcttcgttct tttttattgc ttttatcaat tcaattttag cgtctcaccg
    18421 ggagtcagga aataattctc catgctcatc ccattcagcg acaagatcgt ttctactggt
    18481 acgccagttc gttcggctat ttgatttgga ccttctcctt ccaaaactac ggttgtcgca
    18541 gcctgtgaag tcgcgctgtt ggtattagtt tcagaggaat ctgttgtgtc ttgtgttgtt
    18601 cgagcttgca tttcagaagc ttctaccgac tctgctgtct cagaatcaga agatgttgtg
    18661 tcttgtgtgc ttgttgactc tgcttgaaga gaggcgctag tctgcaaata aatatcggaa
    18721 atcttagcga catgattcgt ataattagcc gataattgat tgatcaaagt atctaagtct
    18781 acaccgtctt tattttctgc tgcctgatac gctgcacgta attttctgat cccttcgtcg
    18841 gagattgctt gaagctcacg tgctttttgg ttagcaactg ctgataaacc agctattccc
    18901 tcttgatttc cctgaatttc tgtctggtat tcttcaatca atgcaggcgt cttttcttcg
    18961 atctttttcg aataatcatt tacgattgct tgaagttgct ctggcaaacc atttgctgtt
    19021 ttttcttcag aagtttgtgt ctcttttgtt ttagttgatg actcacttgt ttgtttcgtc
    19081 tctgttttct ttgtcgttgt ttccgttatg gtcgtacttt tcgcttctga cgaatttttt
    19141 ccatgaaaat atagacccgc aactcctcct gctatgatca aaaacacggc aactgctgcc
    19201 ccaatcatga tcatccgctt acgagctgcc tgtttttgct gtcttcttcg ggtcatccga
    19261 acattttctc cccactcatt ttcatcatac atttttttca ccaccaattt ttatgtataa
    19321 tagcgttatc aaacgcttca tattcttatt gtagcaaata attgtcgctg tgcacatttt
    19381 tatctttgtt atttataaga aaatttcacc agttgagatg attccaagat tgtccgtcga
    19441 tttctccttg cagctgcatt gttcgttcgt ctatctcagc catcagtttc tttatttcat
    19501 ataagagcgc acggtcttga tattctgatt tttcgcttaa ttcatctaat tgttggatca
    19561 gccaatcatt tgtcataatg aagttccttt catttcttgc agtgtaaagg tcaagtcttc
    19621 gatctgctct tttgcctcag tcagtgcttc tacgatatat gcttgttttt cagattcttc
    19681 ttcctgcatt ttctgatttt cctgtactct ttgaataaac cagtcaatcc gctgagtcag
    19741 cgatggtttg ccttccaatg agagatactg ctgataagca attagcttct cccaacgttt
    19801 ttcctgggat aaatacataa attgtgctaa aacgaatggc ggaagatagg tcatatggaa
    19861 tttatttgcg attcgctgat acggcctcaa aaattcggaa atcgtatatt cttcttttcc
    19921 tccagcttgg tattcttttt ccgccacacc tgttgtcaat acgattccta gttctttccc
    19981 ttttaataca tgtgctgctc gttccaagac attatcttgc caaattttta aatgagcagg
    20041 agaactatac cagtaaaacg gaaactggaa aatgatccga tcatgctggg ccaagagctg
    20101 tagttcagtc tctatatcta tttttccgtc tggataactt gattccaaat gatgccaagt
    20161 agctggtaaa ccagcagaag cttctttgaa aaattgctga tttaaagatt cttcgatcgt
    20221 tggatggcta atgatgataa gtgtacgcat cccttttcct cctttttagt cttcgcttta
    20281 cagtgtagca taaccgccac aagtttcttt tgccgatgct tgtcatttta accagactaa
    20341 tcaggttgtc caagaaactg cggtttgttt ttccaactgt tttttcaagg tatccaaagc
    20401 aatattctta aaattttcgt ttttcatacg tttacgatag ttcgtagccg tttctccggt
    20461 actctttttg aacattcggc taaatacgac tagactattg aaccctactt gataggcaat
    20521 actttccacg gattcatccg tcagagaaag aagaaacgcc cctcgattga tccgtacccc
    20581 gatcaaatat tccttgatac tggtacccat atgatttttg aatactcgcg acaaatggct
    20641 ccgattgacc gctaaatgct gggcgatttc ctcaacagac aatttttcag cataatgtgc
    20701 ctcgatgtat ttgactgttt caacagctaa agaggaatga accttttgta caccaccgct
    20761 gacctttccg ccatctttta aaaggactga taaaaaacgg taggttaatg ctgttaactg
    20821 taattcattt tccaaggttt cttccgtatc tttcatgcat tctaaaataa tgtcgatata
    20881 tttttgtatg tcagaagaga ccatcgtgta ttggtcttct ctgaaaagcg actgccgaat
    20941 gatttcttct gcagcactcc caccaaatga aagccagcaa tacatccacg gatcttctcc
    21001 atctgataag taaaacgtcg agtctcctgg acgaatcaag aacagatcac cttttttcaa
    21061 ttggaattgt tgatttttca catgataaga tcctttgcct tccatcacga catgcaggat
    21121 gtaatcattg cgaatagcag gaccaaaaga atgaaacggc atcgttttag agaaaccaca
    21181 aaaatcaaaa tacaattctt tttctacttg ttgatgttcc ttttttaaaa aaatcgattg
    21241 ctccacgtct atccctcctt actcctttag tataatgcaa caaaaagtta aataaaagtc
    21301 acagaaatat attaaaaagg aagcgatttc ttgttaagtt taagtcatta aaagatcttt
    21361 gttattcgtt gtaaccggtt tacttagtat tatatattga acaggagagg aatacagatt
    21421 atgaaaagca aaatgatcca acgtgtgtcc tacgctttag gcgcttttgg acacgacaca
    21481 ttctatgtta cattaagcac ttattttatg gtattcgtga caagccagat gttcgcagga
    21541 acagataagg caacgaatgc aaaaatgatt gctacagtta caagcttagt tgtaggtatc
    21601 cgcttagttg aaattatttt tgatcctttg attggtggca tcatcgataa tactcgtaca
    21661 aaatacggta aattcaaacc atggttagtt atcggcggcg ttgttagttc cgtactcttg
    21721 gtagcaatct ttaccaattt ctttggctta gctactcata atacgacttt atttatcata
    21781 ttatttgcaa tcgttttcat cttattggat agtttttatt catttaagga tatcgctttt
    21841 tggtcaatga ttccagcttt gagtacggaa acaaaggaac gtgaaaaatt tgctacgttt
    21901 gctcgtttcg gctcttctct aggtgcaaat ggtactactc tagtcgttgt ccctatcgtt
    21961 actttcttta cctatcttgt tactcataaa catattgaag gcgcttcagg ctggttctgg
    22021 ttcggtgtga ttgctgctgt tatttcaggc ggtactgcga ttatcactgc cttgggcaca
    22081 aaagaagtcg atactgcgat ccgtcagcaa aatgaaaaag ctgggatcaa agatgtgttc
    22141 aaagcagttg ctcaaaatga tcagttgatg tggcttgccc ttgcttatat cgcttatgcg
    22201 attgccaatg cagcaacaac tgccgtatta ttctacttct tcaaatatgt acttggccaa
    22261 ccaactgaat tttggatcgt tggtctagtt gctgccttac ttggtttagt agctgttcca
    22321 ctattcccaa tgcttacacg cgtcatctct cgtcgctacg tttaccttgg agggatcgca
    22381 atgatgattt cttcttactt tttctttacg atcgcaggtt caagtttgcc aatcgttatc
    22441 atcgggctag tattgttcta cttccctcaa caattgattt tcttatccgc tttgatgacg
    22501 atcacagact ctgttgaata tggtcaatgg aaaaatggta tccgtaacga agctgttact
    22561 ctatcaattc gccctttact agataaaatc gctggtgctt tgtctaacgg gatcgtaggg
    22621 tttgtcgcta tcgcttctgg gatgactggt aatgctactg ctgctgatat cactgctcat
    22681 ggcgtaacga ctttcaaagc ttatgcattc tatgctcctg ctgctttgat gattttctcc
    22741 gctattattt ttgcttggaa gattacattg acagagaaaa aacatgcaca aatcgttgag
    22801 aaattggaaa acaaattggc tccagctgat acaatggaag atgaacttca cgatgcagta
    22861 gatccttccc attaagaaag gatcttgaaa atgaagatta cagaaaaaga ttttggccaa
    22921 ggttatcact tgataacctt ggagaacaaa aacaagctag cactttctat ctctgattta
    22981 ggtgctagaa tcgtcagttt aaaatcaaat gatcgagaat tagttttggg atttgacacc
    23041 gctgaagaat atatcgaaaa agatccgtat atcggtgctt ctatcggtcg caccgcaggg
    23101 agaatcgaaa atggccgctt ttctctcaat ggaaaaactt accaattagc cacagatcca
    23161 aaaactggac ataatctgca tggtggtgct cctggatttg aattaaaaaa atggtcttat
    23221 gtcattttga atggcgaaaa tgaagcttct gtcattttta ctactacaag tcccgacgga
    23281 gaacatggtt ttccaggtac aatggatgtg gaaatccgct atactttgac aaaagataat
    23341 atctggcgag tgacctctcg cggcaccagt gaccaagata ctttattcaa tccgacaaat
    23401 catgtttact tcaacttgac gggagatgct tctcagtcaa tcgatcaaca tgagctttgg
    23461 ctcaatagcg aagcttatgc tcctttgcga acagatagta ttcctatagg ggtaaaagaa
    23521 aatgctgcag gaagtgcttt cgattttcaa atacctaaaa aattagcgtc cgtttttgct
    23581 agtgatttgg atcaaaaaaa tcttgttgac ggaatcgatc atccattttt cttaaaagaa
    23641 accggcttgg gtaaagaagc cgctcgcttg actagccccg ataaaagaat ccaagtagat
    23701 attgctacag atgcttctag tgtggttatt ttcactgcaa atttcggcac agaaacacca
    23761 gaaatgcgca accgaaaact ggcacatcac ggtggcatca catttgagac tcaaacagct
    23821 ccaggtgcag agcgcttctc agcatttggt tcgattcatt taaaagccgg atcagtattt
    23881 gaaacagtaa cagaattcaa aatcaaaaca agaaaggaat aactcatcat ggaaatcaaa
    23941 agaagcttaa acacaaaatt tgccgaaaca ttcggaccta aaaaaactac acaatacttc
    24001 tcaccaggac gcatcaattt gattggtgaa cacacagatt acaatggagg ccatgtcttc
    24061 cccgcttcca tcacttatgg aacgcaaggt gtagcagcac ctcgagacga taataaaata
    24121 ttagtttatt ccacaaactt tgacgatatc ggtgtcatta gttttacttt agatgagcta
    24181 gaatatgata aaaaagcaaa ctgggcaaat tacgtaaaag gaatggtcct taaattaaaa
    24241 gaagctggac actcaatcga tcatgggttt gagttattag tggaaggaac aatccctaat
    24301 ggtgcaggac tttccagtag cgcttcatta gaattattag taggtgttgt attagaagat
    24361 ctgtttgatt taaaaacaga tcgcttggaa ctagtccaaa caggtaaaaa agtagaaaat
    24421 gaattcatcg gtgtcaattc aggtatcatg gatcagtttg ccatcggctt cggtgagcta
    24481 gatcatgcaa tcttattaga tacaaatacg ctgaaatacg aaatggttcc agtgaaacta
    24541 gatggttatg caatcgtcat catgaatacg aataagcggc gggaattggc agattcaaaa
    24601 tacaatgaac gccgtagcga atgcgaagaa gccttggcac gtttgcaaaa aactttgcct
    24661 atctcttctt taggagactt agatgaagaa gaatttgaat caactattga ccaaatcggc
    24721 gatgatacat tgatacgcag agctcgccat gccgtttctg aaaatcagcg gactttgaag
    24781 gcaaaagctg aattagaagc tgggaactta gaagcatttg gtcaactgtt aaatgattct
    24841 caccactctc ttcgctatga ttatgaagta actgggatcg aattagatac actagtagat
    24901 gctgctcaaa aacaagaagg tgtcttaggt gcacgaatga caggagctgg atttggtggc
    24961 tgtgcgattg ctcttgtcaa agaagaaaat atacctgcct tcaaaaacaa tgtctatgat
    25021 gaatacatga aagttgtcgg ctatgctccg gaattttatg ttgcacacat tggaaatggg
    25081 acaacaaaga tcgatccaga agctgttcat gtttgataaa aaaatcaacg atctcagcta
    25141 aaaacaaata gataaaaata acctctcagc aatttccaaa tgtgagcctt ttgctcgtat
    25201 ttgaaaattg ctgtttttta ttgacacgga aagagaacgt gtgttctaat tgtgttgaag
    25261 aggtgattgt tatgtgggcg aatcaaaatc cggtatttgt ttatgaaaaa gaaccttcaa
    25321 gagatatttt atgtattgac tgcaaatctt tttacgcctc cgtagagtgc gttgaaaagg
    25381 atcttgatcc ccttacaact aaattagtag tcatgtccta tccgtccgat tcgacagaaa
    25441 cacggggtag cggattaatc ttggcttcca gtcctgcagc aaaaaaagct tacggcatca
    25501 ccaatatcag ccgtgccaga gatcttccct tcccctatcc aagtgattta tatattgttg
    25561 ccccaaggat ggcctattat atggaaaaga acagagaaat caattctatc tataaaaaat
    25621 atgcagacga agcgaaccat catgtctatt ccgtcgatga aagcttcctt gatgtaacaa
    25681 atagcatggc actctttggt aaaacaaatg cgtacgacat ggctaaactg atccataaag
    25741 atgtgtttga gcagaccgga atctacacga ctataggaat tggcgataac ccgctacttg
    25801 ctaaactagc cttagacaat ggctcaaaag acggaaagca aatgtttgct gaatggcgtt
    25861 atgaagatgt accagaaaag ttatggaaaa tctcagatat cactgacttt tggggaatcg
    25921 gcaaacgaac agcaaaacgt ctgaaacaaa tgggtataca ttctgtttat gatttagctc
    25981 acgcggatta ttatcacttg aaaagcaagc taggggtcat cggtactcag ctttatgccc
    26041 atgcttgggg catcgatcga agttttttgg gacaagaata tacaccaaaa tcaaaaagta
    26101 tcggaaatag tcaaatccta gatagagatt atacaagacg agaagaaatc gagatcgtta
    26161 tcaaagaaat ggccgaccaa gtcggcactc gcttgagacg atctgcagca aaaacacaag
    26221 tagtcagctt atgggtagga tactctttag gctatcttga tccaaatggc aagaccgggt
    26281 ttcaccagca aatgaaaatc gaagcgacaa atgctagcag agatctagca gatgctttat
    26341 tgaccatttt ccaccagcat taccactacc aggatattcg gcgtgtaggc gtgaactgtt
    26401 ccgacctcgt atacgcgaca ggtttacagc ttaacctttt tgaagatcct gaaaagcaga
    26461 taaatgaagc gaaagttgat tttgttgtcg atacgattag aaaaaaatac ggctttcaat
    26521 caatcatcca tgcgcatagt ctcttgcaag gaggacgcgc catcgcacgt agttctctcg
    26581 ttggcgggca tgcaggtggt atgagtggat tagaaggtgg aaaagatgag acggacagca
    26641 aaaaaattca ttgactataa cgagtatcga gatcggccat ttggattaaa atgggggact
    26701 gcttttgcga tggaagaact agtcaacggt atcaaaaaaa tgaagaagaa gcacgcaaac
    26761 agcctcttcc taaacaacaa atgacattgc aagaaatcga tagtatactg acagaagcct
    26821 ttttatctcg taaagcagta atcattcagc tcaatttaaa agatgaattt ggaagactct
    26881 tggatgaggt cgaagggcac ttcattggtg aagcctatga agattacttt atgattgacc
    26941 atcagccgat attgtgggaa gacgtacggt atattgagtt aaaggaaaca aaaaaatggt
    27001 ttgtgacatg agaggttgtg acagaagtga tcaacttcaa aaaataagaa aggatttttc
    27061 gaaaattgct tcttaaattt ttgtggaatt ttggcttatt tttcgaagaa gcaacttctg
    27121 gaacaacgta tattagatat cagaggttgt gaaaaagtcg cttagttttg agtaacaagc
    27181 caaggcgctg atttcacaac ctctgtctat ctatccatct attcggtaag attcatgcat
    27241 gacgtcttct ttatttagta tgtagagccc tttttcttct gaccattcaa cataaatcag
    27301 ctgttttaca tctgcatagc cttctttctt caagttctcc ataagccatg cttcagtcaa
    27361 ttgattggac tgaagcactt tatgctgaat ttgcccttca tctacaagca ggatcgataa
    27421 agctttgtct tcatctgatt ttttcaatac actcatttga ccggcattct ctaggataac
    27481 atgtttggca ttttcaagaa aaaagcattc ttgctgtctt aagattgctc gcaactgctc
    27541 catctcgata tggttatttg tcaactctgt catgttgatg acgccgtctt tgattagaac
    27601 agatggctcg cctttgaccc aacgattgac cttttcattt ttttggacga tacgttcgat
    27661 accataaatc attactgccc aaagcgcaat ggcaaatagg acatgcccta catggacatt
    27721 gtcatcatag gttgattctt ctaagatccc acctaaaaca agcgtataga ctaaatcaaa
    27781 cggtgtgatt tctgacatgg atttcttccc aagcaaacgt accactaata ataaaccgat
    27841 caacccgaac accagtttca ttgtaattga aaaaatcatt ttttctcccc ctaactatct
    27901 gttaattaaa ttatatatgc tttctcacga acacgcatgg aatatacaag gaagaaatgt
    27961 atccttttac taaaaaaagg actgtgacac tgtagtgacc cccaaaagtt agactttttt
    28021 ggagtggaga aatccactcc ttttttttat gctctaaaat gatattttta cacaaccgtt
    28081 acggttgttt tacgaaattg gacg
//
LOCUS       DACGLE010000033        27510 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0534
            SAMEA3304449-rid9560453.denovo.034, whole genome shotgun sequence.
ACCESSION   DACGLE010000033 DACGLE010000000
VERSION     DACGLE010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304449
            Sequence Read Archive: ERR985064
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27510)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27510)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702225.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702225.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 20:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,045
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 27 of 67
            Pseudo Genes (incomplete)         :: 25 of 67
            Pseudo Genes (internal stop)      :: 19 of 67
            Pseudo Genes (multiple problems)  :: 4 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27510
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304449-rid9560453.denovo.034"
                     /strain="VREN0534"
                     /db_xref="taxon:1352"
     gene            complement(<1..188)
                     /locus_tag="IXT20_001509"
     CDS             complement(<1..188)
                     /locus_tag="IXT20_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4859961.1"
                     /translation="MAYSTIGVVVEKSRDNLVFVTEIQTGRAFVVTDKAAKAYQSGDI
                     LTLNLTTKTFVDAAEDYP"
     gene            complement(335..670)
                     /locus_tag="IXT20_001510"
                     /pseudo
     CDS             complement(335..670)
                     /locus_tag="IXT20_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316069.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(682..2274)
                     /locus_tag="IXT20_001511"
     CDS             complement(682..2274)
                     /locus_tag="IXT20_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4859962.1"
                     /translation="MNKSPAVILTCQFTLPNKKSFSTYIDYMTREKALEEMERRTPEE
                     EQELQRIKNALEDFYIPEGETFSESKTDQEFSETSNEAQSLMGNGINFDKLEEQDFTK
                     YLSYMTRHYALEQKKELTQREQKEYQRLNQAIKKYQTEPHKKASGNLPGVFSMASDEV
                     KGSDLKEIKQIFQRGQQKGSIIYQDVVSHDNSYLEKIGLYDPKTDTLDEEGLKAAGRK
                     MMETLFEKEQLNETGYWMATIHRNTKHIHIHFAVVEKENTRKPYTEVENGVSYVVPKG
                     KRRQATLDAMKTNYVHELERFSYEKDRMIGMEKRNLLTRKSDLRNSLTKVVKEPVRYD
                     RHAMNLLNEVYRNLPEKRSEWNYGDENRTKLSPYTREKLDELTQYVLTKEPEYQEYIQ
                     ISKALKEEAKNLYGESKRESKDAEKNALFDLKKRTGNAILTELKRQDSQIKPLIESVD
                     QMQSIYNERSFRTLKQLPTNEYKYSYEEFMKRRKQFQQRVVTQRSLRQLNRTINAHEE
                     KYHASKAYEEQQRRIQMEQERQ"
     gene            complement(2294..2716)
                     /locus_tag="IXT20_001512"
     CDS             complement(2294..2716)
                     /locus_tag="IXT20_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4859963.1"
                     /translation="MAKATRKHVYLYDMHIEVLDEIISTHEDISNYSEAVRHLCMMYD
                     PKIEEKINPIQIKLNAMGKEISILTEMVSEFADLHLKDTGILIGTNSTVYQEAKERVE
                     GKIKANQTKKYSPKIKKSINESVHARVDPFDPTKFLKR"
     gene            complement(2758..3033)
                     /locus_tag="IXT20_001513"
     CDS             complement(2758..3033)
                     /locus_tag="IXT20_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4859964.1"
                     /translation="MDLLIRNIEEKYINKIDKRCEELTVKTGKKWSRNQYLKVLIEND
                     FDHALLNYKKDQFDRLLEKFVDIQTYNTKTLEEYIATNNQLIGLLIE"
     gene            complement(3817..4689)
                     /locus_tag="IXT20_001514"
     CDS             complement(3817..4689)
                     /locus_tag="IXT20_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4859965.1"
                     /translation="MKSTDWIKHTWLPLKRNSKRKEFWILQLWPLGKLCSYLARFILD
                     LIIYSIVVFGTAYYLLNDPNRIAASDIFTVLMTLIFVMLLSIFTQIVMQFTRQKLTAG
                     KLFKSLFDLIIVNAILATLIKQIEQFGRTTKGVKEFLMTFVHFVNGHMYLFEVGIILF
                     LVGYVSTKLIKRSIEKEIEIDLIPTLLGYEYYVDFGRYDELINTDIVHTSIHEATVKG
                     IRTTLKDGQETKAIDRRKEVYNFYEVEANLRYGIGKLSLNGRNIPQNKPYIGWKSDAL
                     EVTKKLPMEEEEIQ"
     gene            complement(4696..4935)
                     /locus_tag="IXT20_001515"
                     /pseudo
     CDS             complement(4696..4935)
                     /locus_tag="IXT20_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332286.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(4953..5183)
                     /locus_tag="IXT20_001516"
     CDS             complement(4953..5183)
                     /locus_tag="IXT20_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4859966.1"
                     /translation="MKRANLVGKDTFAEQKVLIKAYLKVVKAKEICQEVNLSNTTISE
                     LKNGRKSLDTLKFRSFEALYLAAKKYFTAERK"
     gene            complement(5206..6012)
                     /locus_tag="IXT20_001517"
     CDS             complement(5206..6012)
                     /locus_tag="IXT20_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4859967.1"
                     /translation="MSKKKGARQRFKKRKSYLRWLGIFMILGFLILASPIYTHKKVDI
                     PDEISTNAFVNTKELTMVSKEKGANQQLVFEFAIDDEDQSLLKELANLNYRVEVKTAK
                     GDYQAIKAKVIKVSDDYFAVKLSHVPEEYIAMRLTIIPEKIDPKVDMQEPQDLIYYVH
                     EDKVKDQVKDEDYEQHAINYKVKGYKKEQKAAQKQIESFQATIDLNEELVKKLKDQLP
                     YQVADDQENTENKINSYQQEIETSKTQMKDQEEIIQKLQEKIDRMTKENI"
     gene            complement(6023..6667)
                     /locus_tag="IXT20_001518"
     CDS             complement(6023..6667)
                     /locus_tag="IXT20_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4859968.1"
                     /translation="MENKTLKIVLTAAISVSLIGNIALFWQLKEEQETNTFKIEEVAK
                     NASEKLKKNSSEAQAKIDKLNQTINEKNQEIDQLKQQDGTKAKEEVTEAQRKTAEEFG
                     QLAIDKSLSRNELTEKLKDVATQEVIDKLSPADDDHQHDDYGSYSFTLGDVQTYAQTS
                     SVKEEQNLVVFVDYTIGNPQFKDVKPQKIKGGLTVTEKQVDGQWKVTDFSYFTR"
     gene            complement(6683..7888)
                     /locus_tag="IXT20_001519"
     CDS             complement(6683..7888)
                     /locus_tag="IXT20_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HAQ4859969.1"
                     /translation="MENQNESLIKQYVKRRAKRRLFLWLFGTSAGLITILITVFVTLF
                     LILAAGSIDNSDSDSSSGGEAFTGEYSEGLPIYKEIKGRGPFSDEIAQYAVGAAVKYK
                     LLPSVILSQYGYESAFGTSASARNDLNYFGITWFDGCLFPKGTARGIGGIEGGWYMKF
                     PNSKAAFSYYGFMVATQSNFNACVGNKSPGASLLILGRGGYAAAGITEDSPYYTGCMS
                     IITSNKLTEYDEFAIKHWGEGGNNNGTITGEWTNPFPGSSLDKNSFSGGQLFGTNPGG
                     EFRPNGFHDGLDFGSVDHPGSEIHAVHGGKVVYVGNPGISGLGACVIVINYDGLNMVY
                     QEFANSTGNSRVKVGDQVKVGQVIGIRDTAHLHLGFTRMDWRQAQGHAFTDDGTWIDP
                     LPFLNSSKK"
     gene            complement(7888..9912)
                     /locus_tag="IXT20_001520"
     CDS             complement(7888..9912)
                     /locus_tag="IXT20_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HAQ4859970.1"
                     /translation="MLEAIKTAFSPQNDQKVLDPLKQLGNREIEILKSKGYDLDFLKQ
                     VMPQGGLNFDEEYVTYGNGYSRCVTVYHYTKNPTLFWLANLMNNPHTVATLDVSTDEK
                     QKVIDQINRALDELNDRSDNERKATDRNKSAEEYLELAEYARKLTSNGEISKQVKVRV
                     HVYGNTIQEVDERAQELIENLKGMDYRAVTYLHKTKRDWQSMFTMFDEQAEWFGNIKG
                     QSLPSLNIGGGFPFHHQTLKDPGGTQYGETMTGGPFVWNATYKNSVRLSTNTLLLGMM
                     GAGKSTVLKMIAEAHLAAGDFIWGFEKGKDFIPFLKEYNGIMVRLDGSDGMINPLEIF
                     ATRTYDEASSLYDDGSVKDLKINEAASYQTHLDKVVYQVQLVSPQLKGTMKAEFKTYL
                     NRFYEEYGTVPRGFTSSNSRSNTETQVTGKDPEAYPTFKEFLDFLGQLELPGASQEKK
                     NRKEEMESIVESLCETYGMIFDGHSTIRHLDQQQLVFFDIDSITGLDRGVQQCQMYMA
                     MTLIWNQALIQGRRQRRLIKEKQMEEQDFKCFLAMIDECHNAINPDFIETVNYVTNFQ
                     REMRKVEAMTVLATQSPQEMVPENMSSDNFSKLKKVFEFSSTKIFMRMDESILRHIEG
                     LVGKSMTNSELESIPQQNQGDAVINFGSKETIRVHFSPNKRQLKLFDGGK"
     gene            complement(10075..10668)
                     /locus_tag="IXT20_001521"
     CDS             complement(10075..10668)
                     /locus_tag="IXT20_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4859971.1"
                     /translation="MRVKGQNSVDRKSILQFGKPEERSILDLFPYKSCTEEGVLITKE
                     DHFQRYYRVVSTDVEGLNEQEKLERMNQLTTVMRTYVPTIKLVSLTTETDLTEQIVQK
                     RQLLDKNRMEQSLGRNLQRLRKYEKKLVEEIQELKRAEQERPDLSFFFVIEGKTIKDL
                     QTRNRQLMRSSGILGLKPLYKKELIKILYRMNNMNDE"
     gene            complement(10669..11001)
                     /locus_tag="IXT20_001522"
     CDS             complement(10669..11001)
                     /locus_tag="IXT20_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4859972.1"
                     /translation="MDKYIHMKNPPRFSGEWKFFAFFFLDLLTIGGCFFWAKIVNNLV
                     HLPLFFALLNYGLWIAIALVIVWRPYNNPGQRQIFAIIESIFFTDKNHYHALDPNRNY
                     HGTYKEDE"
     gene            complement(11095..11565)
                     /locus_tag="IXT20_001523"
                     /pseudo
     CDS             complement(11095..11565)
                     /locus_tag="IXT20_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014401498.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(11626..13986)
                     /locus_tag="IXT20_001524"
     CDS             complement(11626..13986)
                     /locus_tag="IXT20_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4859973.1"
                     /translation="MIIGGIFSGIADWWSGLFGDGDADPKTVTKVLQDYGNYLQYQNV
                     FSWIYNTFLWGLIKGLYNLNTLLEKTIYQSFDLKDLLNAAGVNELFQSLISKVLALFC
                     VVTLIYLGLKFFMSKHPPKIKNILVNLFMAMILIFGGSSLIDEGLNISKNFYGDVTAA
                     NKADKTGSPAFQIIQNNVIDVSTLLENDPNKVEHIPTDKRNALTAKNFKTANINGIIT
                     PDQAKKMSESSDISTVQKDRLKALQYRLELDDNGKEIPVQISDEGLAKYVYTSGYRRY
                     ISSPGIILAGESSLSVAYLFILFTTVTCIIELVFKKFYLVIAASTDFETGQRMKSAIE
                     DISQSFLLLAFTILELRIYTLMLSGIGDLHAAGKINGFLYVVALIVLTIALFKGSQSV
                     TKIFGVDTSLKNGSNSLLSMFALGNIAKNVGSGAKNLASAGKSGLSNLNDIRKNGFGN
                     RKDVTPDNGEEQSDDKATTVPNSKKTVSEMFGKAKNGVSKAAEGLGYVNERGLKGTAE
                     DLTEKAKDTLEEKLDGTRAKELAEAVKNPSETLEKQKEKASEVKEGLKDAYGEGQVNA
                     AVKANTTTEKENQEGAAIPSAKDHQDELHSNENSEEGQNGQLPKTDPEQYRYKSNINS
                     ENEKRISDLPKRNQEVQTDDDSIDLPNDNGVEVGTEKSTKNGLPKAQNLPMKDSRLNQ
                     FKENDQSFVHQSTAGNVQMKPSRLPENGNLTTVSYNLSESGETGNASVQNVVTREAAP
                     EPIQKVHTGYKPANVAFTAPTKINVTRDTKNRNNALKEQLLKDIKP"
     gene            complement(13983..16592)
                     /locus_tag="IXT20_001525"
     CDS             complement(13983..16592)
                     /locus_tag="IXT20_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HAQ4859974.1"
                     /translation="MRVRETTRRKDKRFNLEKNRYLSHFYRYTPSGEELPEKHDKRIS
                     QKIFYSGLAYLPLNYVVTRIPEAFHRFEIIPGSLQGVNIFQLQEMPQLFHFDSTGFWN
                     PLTPTTSLGTRLVVSTICVVPAVLMGLKMDMWFRPLADGQKGDNRLKTLKEIQQIYPQ
                     IPESKGGFPGYGGIPITHYKKYWYIQTDTVNTCIVGTSRSGKGQIEVLATIDNLSRAE
                     RQSSMVVNDPKTELYVASKDELEARGYDVYAFNITDPLQSMSDNPLALIVKYWKRGDI
                     DTATQLTNTFTNTIYYDANASDNKYFNENAQKAVNALIFALLEQADQTGDYSKLTPNN
                     LVELLSEIGGFNYQDPDNEFKQLNALDEFMNQLPPGNTAKKQYGATKIGSDKAKGNIL
                     STAITGLNPFTLTKIAKMTSQSTIDYKSIGFPKYLDLKLHESLLNQRIVVEFYHQGKK
                     FHEERVKVGYRGFCEINFDCHLEQGDHVCLKYDHRGKEYVAWYEFSQRVLKDEEGNIV
                     YRKKRGETHLPELEKEAVLTLDEDKSNLLVESIRMHYTDKPMAIFMLTPDYDPSNHII
                     VSIFLSQLYKELSTQCVKTKGDKCHRRIHFILDEFGNMPPMDNMEGIMTVTAGRNMLW
                     DLFIQSYQQLYAKYGEQNAAIIKENCQTHIYIMSTDDNTIEEFSKKVGNKTVEQENST
                     INSLGMNTHINRNVDSDRILTPERISTFLEGETIVLAPLKRQDLKGHKIRPYPIFNHG
                     ETNLPYAYQYIEDFNPSWDINDIDIHCTHADLDLKALQIDYDYFLPDIYARRLYHNMH
                     DQIEEEAKENIFIKIVQSLDNSTAQKLLIQFYEEKQIDRLQEYLEKLRQNEEITEVIR
                     KRLLLEAEKAVKE"
     gene            complement(16592..18655)
                     /locus_tag="IXT20_001526"
     CDS             complement(16592..18655)
                     /locus_tag="IXT20_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4859975.1"
                     /translation="MYFTIAGNTKTDKELIKKATKEVGKRTSFLPKTIKYSVIETEEQ
                     LHVLGKVLEHESRSSVKLTLTLHDTEGKIEKAGTEYVFGQLDYQISEGFYPTLMDLIR
                     LDVFKETDETMSVEESYRNEKLLQVLEEELESDQRKVNQGSEVEVNEEVPWERPIFPK
                     KESKEKETVYVDEQADIPTQKSIFEEENDVMGQLEVDPFEETMEDEETAEVEVDPVEA
                     NEESTWTEGDSQEYIEPLISEEVEKNEIEIQKATVFDYILDQYPPEHEWMKEKNKLFL
                     NELYSKYQLPQTYQAFLESKEELISQGKGTLLEQLESVQSTNWHELAQEDLLATFEER
                     QQETDQEINEYATEQSSNWEKAKEELNKEEQRVIEEEVQKIKERYEKKRDNAYSLCVA
                     KINNFTQTNQEALSKEKENLLKERIKERKEEFYRGLRTDKLKISKELNEQLNKLYQTT
                     TEVLMEKHEKIQQELQVQIPKWKKEYEIQQQELKEQEEKQRQKEKEREQLELKKREQD
                     LAEQQMAIEKQRLNQEKEKEAAARKERQEQMDTLRQAQLNYLAQPQPTPSVAAVQTPM
                     NNQTNLTPSPQRSFNGWMVGCIASLSLLLGGGTVFAFNHINQSSQADSVATTTSIKEE
                     AQEQARKEYEQQLEELLNRTNQSTTESSMENSKESDPSKAKEGSSDSTVQSNTSESSE
                     SNEVK"
     gene            complement(18725..19027)
                     /locus_tag="IXT20_001527"
     CDS             complement(18725..19027)
                     /locus_tag="IXT20_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4859976.1"
                     /translation="MFESITMNFLTQPKVVAAVDIFRALQTAATSLKNNLMIFAAAAA
                     VASIVIGGLFFLFGRRGAENGKMHVGYVLMGVGVILAAASIVVSLFGLFGQTPQGL"
     gene            complement(19045..19287)
                     /locus_tag="IXT20_001528"
     CDS             complement(19045..19287)
                     /locus_tag="IXT20_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4859977.1"
                     /translation="MIASLFICPEGGITVAKAKTPFEKAASKILERRGVSETQWKQEV
                     LEKAQFDLLRGKDKDLEGYIYQREREKLVFDEIIKH"
     gene            complement(19284..19748)
                     /locus_tag="IXT20_001529"
     CDS             complement(19284..19748)
                     /locus_tag="IXT20_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4859978.1"
                     /translation="MKKLIIVVTSLIAMIVILVGCSNEKNKQTNQDNGVLKSGTMWKE
                     EVGGLVYNLKIIDETTWEYSESVWHPDPVQITVKRQKDYKGLERYKIVDSAGVREFIN
                     KSDSLFIVVPYEKNGVKKIIFLGSSKDEKRTKEKLIHDGSQSNKYKLQKTSE"
     gene            complement(19775..21865)
                     /locus_tag="IXT20_001530"
     CDS             complement(19775..21865)
                     /locus_tag="IXT20_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HAQ4859979.1"
                     /translation="MNFKHSWTRRLKYVFPIMMMLGTLFGLKTANVFAEKVIVDPSNP
                     IQNVKNNFIIPKDVPNAKTNITVNGASKVYKYHVDANKGGFTDDYVGLVEGNKNIRYP
                     SFQKEYGETNLVLEGVTTNTKVNIDYGKIGTYNGKKVNIKLVLSNIHLYSDTLPWNLL
                     DNNYTKTHFRDDGYKNTNGAMSKSKKRTVLWISDNLFSGIVYHSTQMNVQLVATYEDG
                     SPVQFSGDTFISFNSLNPAGGKTTDLKGEYAHYDKMNTTDWYVRKDTVLSEFKSFYNN
                     LNVVGGHPGGSSKLTQADNDFNNLHDKLGDPKFGQGTVSFKISEANPTFVIGSSNVQT
                     WFTLSSATIFSVVPDQPEKTGVDKNGNNVNDKMLQVGDTIQYRIKQKVNRLGVDLLAV
                     YDRFELIDNLPKEVNYVDAHVESGTNKKFDVSGEVTYDKTKHQVKYAAKADTLKNRMK
                     YNGETYELVINVKVNELANQNSVAKNQGTSIINKVEKDTNIITVYFPKIPVKEVQQNG
                     KDVNGRNDGKKGTPTAPLNAGSEVQYLVTQKWHTKGVDAASDHYKQFSIQDPIEARLT
                     YKEGSAQVIDKSKGKDITSEGTLTYDSNSRTLKWEASADFLSNNLLDGREIQLIFTAK
                     TPLQSEKNIDNQAVVAVDNVSNKTNVVTIGVDPNLPQVIVPKTGSTHLVTISAVSLLL
                     LVLATFSYAVLRYI"
     gene            complement(21862..22122)
                     /locus_tag="IXT20_001531"
     CDS             complement(21862..22122)
                     /locus_tag="IXT20_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4859980.1"
                     /translation="MKLIKLLLFNFSCLTAVFLYCGLLFSKLSILPSYQLYLMRILNI
                     PAASMPLDIMEKLIRNPLFTTINLIVIVGISLVIYKKEGMKG"
     gene            complement(22135..22896)
                     /locus_tag="IXT20_001532"
     CDS             complement(22135..22896)
                     /locus_tag="IXT20_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4859981.1"
                     /translation="MRNRKWLFVCAAALFSFTAYTQLTFADRQESTDSTEQTIQTSDS
                     STIQSSDETVDSSTVDSSEEPKIAEDDQIVKQTIHVQKIISSEQLDNQGQKLEKQEGV
                     NGAKFSVYDVSDILQKMDVKDLTTDQIESQLKDRVKKLFSDQLKLVSNGKTKTIDQQT
                     GVFEFSIEVQANQKQAYYIVNESSPENISNSEDILLLTPVSDKNGEFLKDVWIYPKSE
                     ASQPKEEVKKIVSTGVKKNFFENCWDFVTHLFSRD"
     gene            complement(22912..23664)
                     /locus_tag="IXT20_001533"
     CDS             complement(22912..23664)
                     /locus_tag="IXT20_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAQ4859982.1"
                     /translation="MIIRKPKKKRIMGKWIIAFWLLSAVGVLLLMPAEASVAKYQQNQ
                     QIVAIDRTGTAAETDSSLDVAKIELGDPVGILTIPSISLKLPIYDGTSDKILENGVGI
                     TEGTGDITGGNGKNPLIAGHSGLYKDNLFDDLPSVKKGEKFYIKVDGEQHAYQIDRIE
                     EVQKDELQRNFVTYLEPNPNEDRVTLMTCTPKGINTHRFLVYGKRVTFTKSELKDEEN
                     KKQKLSWTWLLGSTVFLSVMIIGSLFVYKKKK"
     gene            complement(23679..25655)
                     /locus_tag="IXT20_001534"
     CDS             complement(23679..25655)
                     /locus_tag="IXT20_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HAQ4859983.1"
                     /translation="MAINRYVKKAGMLVGMLGIIASCFQGYQAYAEDVTQKTPPEKVN
                     ITVHKLMYDQGTQLNVDIDGIKNDGYTHDQYPTGVTKYNKADYGDVEFTLGNITDQVL
                     PTEDSDLTNAKVDEIVKDVEDKGSNSEYVKNATNKITSAVDENGEITFADQPAYVNSK
                     GNVYVVYESKSAAGLVTQKAKPMVVIAPMTDNTGSGFLKDIHLYPKNIVSKLSFELTK
                     FGDDGTAQSKQTPLKGAKFELYKGEPGKGTKLGDLVSDDQGKLTATDLTLGKYYFVEV
                     PSEVVVGSDKEPTADQYLLGADARNDAHNKLTFEITNDGVTSDLKASYVNYKAPVIDK
                     TVTNGTGQEHSFQIGDAVNYQGTIHIPTDIAGGAGGITVNGVKSETSPYSVFKWGDIA
                     GQGLSYVAAKANIKVTNKDGSVVLKENTDYKIQNSENGFVIDFIVNNGQVSDTVASLH
                     GQDLQMTYNMYVNDSAAVANPLTNSVDFVYNNNPFNQEEHHEKTKADVVTYGAKFLKV
                     DSGLFGIGIKATPLEGAEFAAKNAEGKYYGGLVDTDKNGVKEAVWVDDVANAAILKSD
                     KEGHFEITGLTEGEYSLEETKAPENYQKLTKEISFKVDKDSYKEENRITIKNNQKASV
                     PMTGSNGFQTYVLISCLLLGAGALSAVVYFKKKA"
     gene            complement(25708..26382)
                     /locus_tag="IXT20_001535"
     CDS             complement(25708..26382)
                     /locus_tag="IXT20_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HAQ4859984.1"
                     /translation="MDEKMVIFFLGVALIVFCSVFGYVSYQKHEGEVFKQNIEKKIPV
                     DQINAHAKSYKEDATNVNNDMSLGQMLSIQKEAIEMGVNKQVFAQIQIPALGLALPIF
                     KGTNQYTLSLGAATYFYEDGEMGKGNYVLAGHNMEMPGVLFSDIQKLSLGEVMDLVSN
                     DGVYRYKVTRKFIVPEYFKLIDGVPEENSFLSLPKKGEKPLLTLFTCVYTSQGKERYV
                     VQGELQ"
     gene            complement(26459..>27510)
                     /locus_tag="IXT20_001536"
     CDS             complement(26459..>27510)
                     /locus_tag="IXT20_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014401493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="EpaQ family protein"
                     /protein_id="HAQ4859985.1"
                     /translation="YDIIYDNAAQLVTFFVGCLFLLNLYKVKRNDLFFIAAGILFYLF
                     FSFFRSYRVDTFYGDLIVPTIIAIILIVKWTEFTVFDRSLYLVVFYVFLLVTVYRVFT
                     EIPVPEGQSIWNPSNKLSDIWINTNTIGSSLMTLALLITGFASSFVKWYMRLLSLPAL
                     ILAGATIWVCQSKAALYALVIFVILEILPKKIFRNTHLSFWMYAAVSVLALPISYFAA
                     MSDKVHLFTGREEIWRKFYETLGEKTGQIFVGMKPFFFHIGNQILGNHNTYHALVNHY
                     GLIGFALSIIPLLSFLWLLVRKCELTNGQLTFLWAFLAIMCQSFMEETLTTISWVPIV
                     FLMLAMATHQYKTTR"
BASE COUNT         7542 a         5556 c         4297 g        10115 t
ORIGIN      
        1 aatggataat cttctgccgc atcgacgaac gtcttcgtag tcagatttaa cgttaaaata
       61 tcgccacttt gataagcttt tgcagctttg tctgtgacta caaaagctct gcctgtttga
      121 atttctgtca caaaaacgag attgtctctg cttttttcaa caacaactcc aatagttgaa
      181 taagccattt ctgcttcctc ccttctcttt cttttgatat aatgtagtcg gaaatcgcac
      241 cactcaatga attggcggaa aacaattcag tgaaaaaaaa gagtcctgga caatcacgtt
      301 caggactctt ttacttcttt tattcactta ataactaaca ctctgtgagt gaaatagact
      361 cttttatgct tttacatgga aagttattga ttccctcttc attcatttcc caagtagtat
      421 aataaggtgt taacttggcc tcatattctt gaatgataaa atatttttca ccgttcacat
      481 gattacaaca atacacacca gtagctgggt actcaacgac taaaaattaa tttggatcaa
      541 tctgttcgct tctcaacatt tctcttatct gaaacaaatg ctctttttcc aaacaatcgg
      601 gaagttgttc gattcgttct ttttttagtg atccttctat gactttccat tgattcaaat
      661 atcgtttcat ttctcttcct cctactgtct ttcttgttcc atttgaattc ttcgttgttg
      721 ctcttcgtat gctttacttg cgtggtattt ttcttcatgg gcattaatcg ttcgatttaa
      781 ttgacgcaaa ctccgctgcg tgacaactct ttgttggaat tgtttcctgc gcttcatgaa
      841 ttcttcgtaa ctatatttgt attcattcgt tggcaattgt ttaagcgttc gaaaggatcg
      901 ttcattgtaa atagattgca tctgatcaac actttctatt aatggtttta tttgactatc
      961 ctgtctcttc agctctgtta ggatggcatt tcctgtgcgc ttttttaaat caaagagagc
     1021 atttttctct gcatcctttg attctctctt gctttcccca taaagatttt ttgcttcttc
     1081 tttcaatgct tttgaaatct gtatatattc ttggtattca ggttcttttg ttaacacata
     1141 ttgggttaac tcgtcgagtt tctctcttgt gtatggtgaa agtttggttc gattttcatc
     1201 cccataattc cattccgaac gtttttcagg taagtttcta tatacttcat ttaataagtt
     1261 cattgcgtgt cgatcataac gtactggctc ttttaccact tttgttaagc tgttacgcaa
     1321 atcacttttt cgtgtcaata aatttcgttt ctccatacca atcattcgat ccttttcata
     1381 agagaaacgt tctaactcat gcacataatt ggttttcatt gcgtctaatg ttgcttgcct
     1441 tctctttcct ttcggtacaa cataactcac accattttca acctcagtgt aaggtttacg
     1501 cgtattttct ttttcaacaa ctgcaaaatg aatatggata tgtttagtgt tacggtgaat
     1561 cgttgccatc cagtagcctg tttcgttcag ttgctctttc tcgaataatg tctccatcat
     1621 ttttcttcca gctgctttta acccttcttc atctaaagta tctgttttgg gatcgtacaa
     1681 tcctattttt tcaagatacg aattatcatg agagacaaca tcttgataaa tgattgagcc
     1741 tttttgttgt cctctttgga agatttgctt aatctccttc agatcacttc ctttcacttc
     1801 gtctgaagcc atagaaaaaa ctccaggaag gtttcctgaa gcttttttgt gtggctctgt
     1861 ttgatatttt tttattgctt ggttcaagcg ttggtattct ttttgttccc tttgcgtcag
     1921 ttctttcttt tgttctaacg catagtgacg ggtcatataa gaaagatatt tggtaaaatc
     1981 ttgttcctct aacttgtcaa agttgattcc atttcccatc aaagattgag cttcattcga
     2041 cgtttcgctg aattcttgat ccgtcttact ctccgaaaaa gtttctcctt caggaatata
     2101 aaaatcctct aaagcattct tgattcgttg cagttcctgt tcttcttcag gcgtacgtcg
     2161 ttccatttct tccagcgctt tttctcttgt catataatca atgtatgttg aaaaagattt
     2221 tttattgggc aaggtaaatt gacaagtgag aatgactgca ggcgatttat tcatcatgtt
     2281 ccccttctac tttttatctt tttaaaaact ttgttggatc aaacgggtct actctggcat
     2341 gaacagattc atttattgat ttcttgattt taggagaata cttcttcgtt tgattcgctt
     2401 ttattttccc ttccacacgc tcttttgctt cttggtaaac agttgaattt gtaccaatta
     2461 aaatccctgt atctttcaaa tgaagatccg caaactctga aaccatctcg gttaaaatag
     2521 aaatttcttt tcccattgca ttcaatttaa tttgaattgg atttattttt tcttcgattt
     2581 ttggatcata catcatacac aaatgtctga ctgcttcact ataattagat atatcttcat
     2641 gagtagaaat tatttcatca agtacttcga tatgcatatc gtaaagatat acatgttttc
     2701 ttgttgcttt tgccaaaatt gatttcctcc ttttataaat tcacaatact atttttatta
     2761 ttcaattaac aaaccaatta attgattatt tgttgcaata tattcttcta aagtttttgt
     2821 attataagtt tgaatatcca caaatttttc taacaaccga tcaaattggt cttttttata
     2881 attcagaaga gcgtgatcaa aatcattttc gatcaaaact tttaggtatt gatttcgact
     2941 ccattttttg ccagtcttga ccgtaagttc ttcacaacgt ttgtcaattt tattaatgta
     3001 cttttcttca atatttctaa ttaataagtc catattttcc tccaaattta ttatgtgtag
     3061 ggatatgtgt agctacacat atccctacac attttttgat tttgctacac acaaacgctt
     3121 attttactgc ccacattcgc tttcatccta cccacattcg ctttcatcct acccacaaat
     3181 ctacacacaa atccaaaatt ttgatatcat ttatctttct tagaaacgtg gtaggcacag
     3241 cctacctagc acctttcgta agaaagtgat atcacgtttc cttatgctct ttaaaaatta
     3301 aggttatttg tgtatttaga caataaaaaa tccttctaac tttgtcgatt agaaggaaat
     3361 attttgtcat atgaaaaatg aatttaagaa aaagtgtatg ctataattga tacgctgtct
     3421 tcctgtttgg ttgaaatctt cagccagaaa ggaggagtgt taaatgtttt atgtgcttga
     3481 tgtttgcgtt agaatcgtgg ttaatgtttt ctcccatctc attaacaaat ggttagattc
     3541 taaacatgat aaagacagct agcctgttca ttatttggtt aatagtgaat aaagaaaaga
     3601 gccttacgca gctgcaactg ctagggctct ttttgagtat taaatgtttt atgtgtttgc
     3661 attatcagta tagcatacac ttttttcaca gacaactttc tacatttttt cttctttttt
     3721 caatcaaaat aaactaatga ataatataaa ctcttattga gaactgttgg aaatgattac
     3781 tgatatcttc aaacaaatga ttcaaattga ctagactcat tgaatctcct cttcttccat
     3841 aggaagcttt ttagttactt ctaaagcatc acttttccag ccaatgtatg gtttgttttg
     3901 tggaatattt cttccattta acgacaattt tccaatgcca tatcttagat ttgcttcgac
     3961 ttcataaaaa ttgtagacct cttttcttcg gtcgattgct ttcgtctcct ggccatcttt
     4021 taacgtagtt ctgatgccct taacggtagc ttcatggata ctcgtgtgaa caatatctgt
     4081 attgatcaat tcgtcatatc ttccaaaatc aacatagtac tcataaccaa gaagtgtcgg
     4141 aatcaagtca atctcaattt ctttttcaat tgaacgttta ataagtttcg tggatacata
     4201 cccgactaaa aagagaataa tacccacttc aaacaaatac atgtgcccat ttacaaagtg
     4261 aacaaacgtc attaaaaatt ccttcactcc ttttgttgtt cttccaaatt gttcgatttg
     4321 ttttattaaa gttgctagaa ttgcatttac aataattaaa tcgaatagac ttttgaaaag
     4381 ttttcctgct gttaattttt gcctggtaaa ttgcatgact atttgagtaa aaatagacaa
     4441 tagcatgaca aaaatcaacg tcattagcac tgtaaaaatg tctgatgctg caatcctatt
     4501 gggatcattt aataagtaat aagccgttcc aaatactaca atcgagtaaa tgattaaatc
     4561 aagtataaaa cgagccaaat agctacaaag tttacctaat ggccataatt gtagaatcca
     4621 aaattcttta cgtttgctat ttcttttcaa aggaagccaa gtgtgtttga tccagtcggt
     4681 cgattttatt tcaatttctt ctctcatttc tttcttcttt tcttgccaac gagaaggcag
     4741 aagtttgttg atttctgtcc ccaacaaaat caataggacg catgagatta cactcatgag
     4801 tcccttcaat aataaatctt cattagtaaa ccatagaaaa atcgttccca aggtcacggc
     4861 tacaataaaa aagaagccaa cgatacctac ggtgatatag ctgatccata aacatttttg
     4921 atacactttt ttcacataaa atcctcctgt ttttattttc tttcagccgt aaaatatttt
     4981 ttagcagcta aatacaaagc ttcaaatgat ctgaatttta aggtatctaa actctttcta
     5041 ccattcttca actcagatat tgttgtgtta gataaattca cttcttgaca tatttcttta
     5101 gcttttacaa ctttgagata ggctttaatc aatactttct gctcagcgaa agtatctttg
     5161 ccgaccaagt tcgctcgctt catttctttt actccaccta ttcttctaaa tattttcttt
     5221 tgtcatacga tcaatttttt cttgaagttt ttgaatgatc tcttcttgat ctttcatttg
     5281 cgttttcgat gtttcaattt cttgctggta actattaatc ttattttcag tattttcctg
     5341 atcgtctgca acttgataag gcaattgatc ttttagtttc ttcactaatt cttcattcaa
     5401 atcaatggtt gcttggaagg attcaatctg tttttgtgct gctttttgtt cttttttgta
     5461 tcctttcact ttgtaattga tcgcatgttg ttcataatct tcatctttca cttgatcttt
     5521 aaccttatct tcgtgaacgt aataaatcag atcttgcggt tcttgcatat ccacttttgg
     5581 atcaattttt tcaggaataa tggttaaacg catggcaata tattcttctg gtacatgaga
     5641 tagcttcact gcaaaataat catcagacac tttgatcact ttggctttta ttgcttgata
     5701 atccccttta gctgtcttta cttctacgcg ataattaaga ttagccagtt cttttaataa
     5761 gctttgatct tcatcgtcga ttgcaaattc aaaaactaat tgctggttcg ctcctttttc
     5821 ttttgaaacc atcgtcagtt ccttcgtgtt tacaaatgcg ttcgtgctta tctcatctgg
     5881 tatgtctacc ttcttatgcg tataaattgg actagcaaga atcaaaaagc cgagaatcat
     5941 aaagatcccc agccatctaa gataactttt tcgttttttg aaacgttgtc ttgctccttt
     6001 tttcttactc attttcttcc tcctaacgag tgaaataact aaagtctgtg actttccatt
     6061 gaccatctac ttgtttttca gtgacagtta atccaccttt tattttttgc ggtttgacat
     6121 ctttgaattg aggattgccg attgtataat caacaaatac gacaagattt tgttcctctt
     6181 ttacactgct tgtttgagcg taagtttgca cgtcacctaa tgtaaaggag taacttccgt
     6241 aatcatcatg ttggtgatca tcgtctgcag gacttaattt gtcgatcact tcttgggttg
     6301 cgacatcttt tagtttttcc gttaattcat ttctgcttaa tgatttatca atggccaatt
     6361 gaccgaactc ttctgccgtc tttctttgtg cttcagttac ttcctctttt gcttttgtgc
     6421 catcttgttg cttcagctga tcgatttctt ggtttttctc attgatggtt tgatttaact
     6481 tgtcgatctt cgcctgtgcc tcagaggaat tttttttgag cttctcagac gcgtttttag
     6541 ccacttcttc aattttaaag gtatttgtct cttgttcttc tttcagctgc caaaacaacg
     6601 cgatattgcc gattaaacta acgctaattg cagctgttaa gacgattttt aaagttttat
     6661 tttccatatt ttttctcctg atctattttt tgctgctatt caaaaatggt aatggatcaa
     6721 tccatgtacc atcgtctgtg aatgcgtgtc cttgtgcttg acgccaatcc attcgagtga
     6781 aacctaaatg caagtgtgct gtatctcgta tcccgattac ttgaccaact ttcacttgat
     6841 cgccaacctt cacacgagaa tttcctgtac tattcgcaaa ttcttgatat accatattca
     6901 aaccgtcgta attaatcacg atcacacatg cacctaaacc tgaaatacca ggattgccaa
     6961 cgtacacgac ttttcctcca tgcaccgcgt gaatttcact tcctggatga tcgactgaac
     7021 caaagtctaa tccatcatgg aagccattcg gacggaattc accgcctgga tttgttccaa
     7081 ataattgacc accactgaag gaatttttat ctaaactgct gccagggaat ggattcgtcc
     7141 attcaccagt gatcgtgccg ttgttattgc caccttcacc ccaatgtttg atggcgaatt
     7201 catcatattc ggttaattta ttagaagtga taatggacat acatcctgta taatacggac
     7261 tatcttccgt aattcctgca gcggcataac cgcctcgtcc taagatcaat aaacttgcgc
     7321 cagggctttt atttcctaca catgcattaa aattggattg tgtggctacc ataaagccgt
     7381 aatatgagaa ggcagctttt gagttgggaa acttcatgta ccagccacct tcaatgccac
     7441 caattcctcg tgcagttcct ttaggaaaca gacaaccatc aaaccaagta atcccaaaat
     7501 agttgagatc atttcttgcg gacgccgacg taccaaacgc tgattcataa ccgtattgcg
     7561 aaaggatcac acttggtaat aatttatatt tcactgctgc acctacagca tattgcgcaa
     7621 tctcatcaga aaaagggcct cttcctttga tttctttata aatcggtaag ccttccgaat
     7681 attctcctgt aaaagcttcg ccaccactac ttgaatctga atcagaatta tcaatcgaac
     7741 ccgccgcaag tattagaaaa agggtcacga acacagtaat caaaatagta atcaaacctg
     7801 cagaagtacc aaacaaccac aaaaataagc gtcgctttgc acgacgctta acatactgct
     7861 tgattaaact ttcattttgg ttttccatta ttttcctcca tcaaacaatt ttaattgacg
     7921 tttgttcgga ctaaaatgaa cacgaatggt ttcttttgag ccaaaattga tcacagcgtc
     7981 tccttgattt tgttgaggaa tggattctaa ttctgagttt gtcattgatt taccaactaa
     8041 tccttctata tgacgtaaaa tagattcatc catacgcata aagatcttcg tagaagaaaa
     8101 ttcaaagact ttcttcagtt tcgagaagtt atcagaagac atattttctg gcaccatttc
     8161 ttgtggggat tgtgtagcta aaacagtcat tgcttctact ttacgcattt ctcgttgaaa
     8221 gtttgtgaca taatttaccg tttcaataaa atctggatta atcgcattat ggcactcatc
     8281 aatcatcgcc aagaagcatt tgaagtcttg ttcttccatt tgtttttctt taattaaacg
     8341 tcgttgtctt cttccttgaa tcaatgcctg attccaaatc aacgtcatag ccatgtacat
     8401 ttgacattgt tgtaccccac gatctaaacc tgtaatagag tcaatgtcaa aaaagaccaa
     8461 ctgttgttgg tcaaggtgtc gaatcgtact atgaccatca aaaatcatgc catacgtctc
     8521 acataaggat tctacgatgg attccatttc ttcttttcga ttctttttct cttgggaagc
     8581 acctggtaat tccagttgtc ctaaaaagtc taaaaattct ttgaacgtcg gataagcttc
     8641 tggatctttc cctgtgactt gcgtctccgt atttgaacgc gagtttgagg aagtgaaacc
     8701 gcgtggtact gttccatact cttcataaaa acgatttaaa taggtcttaa attctgcttt
     8761 catcgttcct tttagctgag gactcactaa ctgtacctga tagaccactt tgtctaagtg
     8821 cgtttgatag gacgctgctt cattgatctt caaatctttg actgatccat cgtcatataa
     8881 actggaagct tcatcatacg tacgtgtggc aaatatttct aaaggattga tcatcccgtc
     8941 agatccgtct aaacgcacca taatgccgtt atattccttt aaaaagggaa taaagtcttt
     9001 tcccttttca aagccccaaa taaagtctcc tgcagctaaa tgggcttccg cgatcatttt
     9061 aagaacggtc gatttaccag ctcccatcat tcctagaagc aaggtattgg tagacaaccg
     9121 cacactattt ttatacgtgg cattccaaac aaacgggccg cctgtcatgg tttccccata
     9181 ttgtgttcca cctggatctt ttagcgtttg gtggtggaac gggaaacctc cgccaatgtt
     9241 tagcgatggt agtgattgcc ctttgatatt tccaaaccat tcagcttgtt catcgaacat
     9301 ggtgaacatc gattgccagt ctcttttcgt tttgtgcaga taggtcactg cacgataatc
     9361 catccctttg agattttcga tcagttcctg tgctcgttca tctacttctt gaatcgtgtt
     9421 tccgtaaaca tgtactctca ctttgacttg tttagaaatt tccccattag aagtcaattt
     9481 tcgggcatat tccgctagtt ctaggtattc ttccgcgctt ttatttcggt cagtcgcttt
     9541 tcgttcatta tctgaccgat catttaattc atctaaggca cgattgattt gatcaatcac
     9601 tttttgcttt tcatctgtgc taacatctag tgttgccact gtatgaggat tgttcatgag
     9661 attggccaac caaaaaagcg taggattttt tgtgtagtga taaactgtca cacatcgtga
     9721 ataaccattt ccataggtca catactcttc atcgaaattt aatccacctt gtggcatcac
     9781 ttgttttaaa aagtctaaat catacccttt ggattttaaa atttctattt cccgatttcc
     9841 tagttgcttt aatggatcaa gtaccttttg atcattttgt ggtgaaaaag ctgttttaat
     9901 tgcttctaac atgtacagtc acctcttctt gtttgattct ttacttggtt aaaagatttt
     9961 ccttttccca acttacccag gcttaaagtg tgaggaaatt ttctagtggt ctcttctttc
    10021 ggaacatctt tttctaacca tatcaacgga tatttcattc tctcttcccc attcctactc
    10081 atcattcata ttattcattc gataaagaat cttgatcaat tcttttttgt agagtggttt
    10141 taaccctaaa atgccactag atcgcattaa ctgacggttt ctggtttgca aatccttgat
    10201 tgtctttcct tcaatgacga aaaagaagct taaatcaggg cgttcctgtt ctgctcgttt
    10261 caattcctgg atttcctcta ctaatttctt ttcatatttt cttaatcgct gtagatttct
    10321 tcctaaggac tgttccatac gatttttatc taataactgc cgcttttgca cgatttgctc
    10381 cgttagatcc gtttctgtgg tcaaactaac taacttaatc gttggcacat acgtacgcat
    10441 gacagtggtc aactgattca ttcgttccag tttctcttgt tcatttaatc cttccacatc
    10501 tgtagaaact acacgataat aacgttgaaa atgatcttct ttagtaatta agactccctc
    10561 ttctgtacag gatttataag gaaataagtc caagatagag cgctcttctg gttttccaaa
    10621 ctgcaaaatg gattttctgt caactgaatt ttgacctttg actcgcattt attcatcctc
    10681 cttatacgta ccatgataat ttcggttagg atctaacgca tgataatggt tcttgtccgt
    10741 aaaaaaaata gactcgataa tcgcaaaaat ctggcgttga cctgggttat tatagggtcg
    10801 ccaaacaatc actaaagcta tcgcaatcca taagccataa ttcaagagtg caaaaaagag
    10861 tggtaagtgg acaagattat tgactatttt cgcccaaaaa aagcaaccac caatcgttaa
    10921 tagatctaaa aagaaaaagg cgaaaaactt ccattcccca ctaaatcttg gtggattttt
    10981 catatgaatg tatttatcca taatgtaacc atactcctta tgttctttat ttttagaaac
    11041 aaaaaacact gtgatttctc acagcgtttt tctaaatatt ataactctaa ttcaattttc
    11101 ttcttttaat tcaaaaattt tttgttctag gtttcttttc atttgaatca actcataatt
    11161 ttgtgcgcgt aattcttcgt tttccaaatg aactcgttgc atttcatcgt ttaatcgatt
    11221 taaattctcc aatgattctt gtatcgtttc ttgcctaaaa ttaagtgagc caagttgacg
    11281 atctttttgt tgtctttgct ctttcaattc tgacgatatt tgctcaacag aatgaaatag
    11341 ctccgttaat tttactgtga atgtgggcat agcatctttc aaataagtga atccttctga
    11401 aaggtgtaca ataatgcttt cggtatgtgt tttttgacca tctaaaatag actgatgatc
    11461 tttttgtaaa ctctccgtat ctttaaatcc tttaatagtc gccacaaata tcgttaaaaa
    11521 tgctcccagt gcagttataa acgtagctat taaaatatcc tccacaattt tctcctttcc
    11581 attttaaact tgttttataa agaaaaaggc ccttaaatag gccttttatg gtttgatatc
    11641 cttcaatagt tgttctttca atgcattgtt cctgtttttg gtatctcttg tcacatttat
    11701 ttttgtcggt gcagtaaatg ccacatttgc tggtttgtaa cctgtgtgaa ctttttgaat
    11761 aggttctggt gcagcttctc ttgtcacaac attttgaacg gacgcattcc ctgtctcccc
    11821 actttcagat aaattgtaag aaacagttgt cagatttcca ttttcaggca aacgacttgg
    11881 tttcatttga acattacctg cagttgattg gtgaacaaag ctttgatcat tctccttgaa
    11941 ttgattcaaa cgactatctt tcattggtaa attttgtgcc ttaggtaaac catttttcgt
    12001 tgatttttca gttccaactt cgaccccatt atcatttggc agatcgatgg aatcatcatc
    12061 cgtttgtact tcttgatttc gttttggtaa atctgaaatg cgcttttcat tttcagaatt
    12121 aatgttagat ttataacgat attgttctgg atctgttttt ggtaattgac cattttgtcc
    12181 ttcttctgaa ttttcgttag aatgcaactc atcttggtga tctttggctg aaggaatcgc
    12241 tgcaccttct tgattttctt tttcggttgt tgtattagct tttactgctg cattgacttg
    12301 tccttcacca tacgcatctt ttagtccttc tttgacttct gaagcttttt ctttttgttt
    12361 ttctagtgtt tcggatggat tcttcacagc ttcagccaat tctttagctc tcgtgccgtc
    12421 taatttctct tctaaggtgt ctttggcttt ttctgtaaga tcttcagctg ttccttttaa
    12481 cccacgttca ttgacgtaac caagaccttc tgcggcttta gaaacaccat ttttcgcttt
    12541 cccgaacatt tcggaaactg ttttcttact attaggtaca gttgtagctt tatcgtctga
    12601 ttgttcttcc ccgttatcag gggtaacatc ttttcgatta ccaaatccat ttttacgaat
    12661 atcatttaaa ttgcttaaac cactcttacc agcacttgca aggtttttag ccccgctacc
    12721 cacatttttc gcaatatttc ctaaggcaaa catgctaaga agagaattgc tgccgttttt
    12781 cagactcgta tcaacgccga aaattttcgt aactgattga gagcctttga atagcgcaat
    12841 ggttaataca attaaagcga caacatacaa gaatccattg atctttcctg ccgcatgcaa
    12901 atcaccgata cctgaaagca ttaatgtata gattcgtaat tctaaaatcg taaatgccaa
    12961 taacaagaag gattgagaaa tatcttctat agcacttttc atccgttgtc ctgtctcaaa
    13021 gtccgttgac gcggcaatca ctaaatagaa ttttttaaat accagctcaa taatgcaagt
    13081 aacagtcgta aacaagataa ataaataggc taccgacaaa gaagattcac cagctaaaat
    13141 aatacctgga ctactaatgt atcggcgata gcctgaagta tacacatatt ttgctaaacc
    13201 ttcatcacta atttgaacag ggatttcttt cccattatcg tctaactcta gtcgatactg
    13261 taatgccttt aaccgatctt tttgtactgt actaatatca cttgattcag acattttctt
    13321 agcttgatca ggtgtaataa tcccattgat attcgctgtt ttaaaatttt tcgccgtgag
    13381 agcatttctt ttgtctgttg gtatgtgttc tactttgttc ggatcatttt ctagtaacgt
    13441 actcacatca atcacattat tttgaataat ttgaaaagca ggtgaaccag ttttatctgc
    13501 tttatttgct gcagtcacgt ctccgtaaaa gtttttacta atatttagcc cttcatcgat
    13561 taaagaacta ccaccaaaaa tcaaaatcat ggccataaat agattaacca agatattttt
    13621 gatttttggt ggatgtttac tcataaaaaa ttttaagcct aaataaatta atgtgacgac
    13681 acaaaaaaga gccaatacct tactaatcaa actttgaaac agttcattaa caccagcggc
    13741 gttcaataag tcttttaaat caaagctttg ataaattgtt ttttctagta aagtattgag
    13801 attatataac cctttaatca aaccccataa aaaagtatta taaatccaac tgaatacatt
    13861 ttgatactgc agataattac cataatcttg taagactttc gttacggttt tgggatctgc
    13921 atccccatcc ccaaataagc ctgaccacca gtcggcaatg ccggaaaata ttcctccaat
    13981 gatcattctt tcaccgcctt ttcagcttct agcaaaagtc gtttccttat aacctctgta
    14041 atttcttcat tttgacgcaa tttttcaagg tattcttgca aacgatctat ttgcttctct
    14101 tcataaaatt gaataagtaa cttttgtgcc gttgagttgt ctaaagattg aacgatttta
    14161 ataaaaatgt tttctttagc ttcctcttct atttgatcgt gcatattgtg ataaagtcga
    14221 cgtgcataga tatcaggtag aaaataatcg taatcaattt gtaaagcctt aagatccaag
    14281 tccgcatgtg tacaatgtat gtctatatca ttgatatccc acgatggatt gaaatcttca
    14341 atatattgat aagcataagg aagattcgtt tccccatgat taaaaatagg atacggccgt
    14401 attttatgac ctttcaaatc ttgtcgtttt aaaggagcga gtacaatcgt ttcaccttct
    14461 aaaaaagtag aaattctttc aggagttaag atgcgatctg aatcaacatt gcgattgata
    14521 tgagtattca tacctaatga attgatggta ctgttctctt gttcaaccgt cttattcccc
    14581 acttttttgg aaaattcttc tatggtatta tcgtctgtcg acataatata aatatgtgtc
    14641 tgacaatttt ctttgataat cgctgcgttt tgttccccat acttcgcata aagttgctga
    14701 taggattgga taaacaaatc ccacaacatg tttctaccag ctgtgactgt catgattcct
    14761 tccatattat ccattggtgg catattccca aattcatcta aaataaaatg aatcctacgg
    14821 tgacatttat ctcctttagt cttaacacat tgcgtagata attctttata caactgacta
    14881 agaaaaatac taacaatgat atggttagat ggatcataat caggtgtgag cataaaaata
    14941 gccattggtt tatcggtata gtgcatccga atagactcta ctaataagtt acttttatcc
    15001 tcatctaatg ttagaacggc ttctttttct aattctggca aatgggtttc tcctcgtttt
    15061 tttcgataaa cgatatttcc ctcttcgtct ttcaatactc tttgagaaaa ctcataccaa
    15121 gcaacatatt cttttcctcg atgatcgtat tttaagcaaa catgatctcc ctgttctaaa
    15181 tgacaatcaa agttaatttc acaaaaacct ctatagccta ctttgacacg ttcctcatga
    15241 aatttttttc cttgatgata aaactctaca acaattcgct ggtttaacag actctcgtgt
    15301 agtttgagat ccaaatactt cggaaaacca atggacttat aatcaatggt tgattgagaa
    15361 gtcattttcg caattttcgt cagtgtaaat ggatttagtc ctgtgatcgc tgtcgataga
    15421 atgttgcctt tcgctttatc agaaccaatt tttgttgccc catattgttt ttttgctgtg
    15481 tttccaggtg gtaactgatt cataaattca tctaaagcat tgagctgctt gaattcatta
    15541 tcaggatctt ggtaattaaa accaccaatc tcagaaagaa gttcgacgag attattcggt
    15601 gttaatttgg agtaatcccc tgtctgatcc gcttgttcca ataacgcaaa aataagcgca
    15661 ttgacagctt tttgcgcatt ttcgttaaag tatttattgt ctgaagcgtt ggcatcataa
    15721 taaatcgtat tcgtgaaagt attggttaac tgtgtcgctg tgtctatgtc tcctcgcttc
    15781 cagtatttca cgatgagtgc caatggatta tcactcattg attgcagtgg atcggtgata
    15841 ttaaatgcat acacatcata ccctctagct tctagttcat ctttactagc aacatataat
    15901 tctgtttttg gatcattaac aaccattgaa ctttgtcttt ctgcacgaga caaattgtca
    15961 atggttgcta aaacttctat ctgtcctttc cctgaacggg aagtaccaac aatacaagta
    16021 tttactgtat ccgtctgaat ataccagtac tttttataat gagtaattgg tattccgcca
    16081 taaccaggaa aacctccctt tgattctggt atttgcggat aaatttgttg gatttccttt
    16141 aacgttttta atcggttatc tcctttttgc ccatctgcta gaggacgaaa ccacatgtcc
    16201 attttcaatc ccataagtac agctggaaca acacaaatcg tagaaacaac taaacgtgtg
    16261 cctaaacttg ttgttggggt caaaggattc caaaaaccag ttgaatcaaa atggaataac
    16321 tgcggcattt cttgtaactg aaaaatattc actccttgta aacttcctgg aataatctca
    16381 aatcgatgaa atgcttctgg tattcttgta acaacatagt ttaaaggaag ataagctaag
    16441 ccactataaa aaatcttttg agaaatacgt ttgtcatgtt tttctggtag ctcttctcct
    16501 gaaggagtat agcgatagaa atgagaaaga tacctattct tttctaaatt gaatcgtttg
    16561 tcttttcgtc tcgttgtttc tctaacacgc attatttcac ctcatttgat tctgaacttt
    16621 cggaagtgtt tgattgtaca gttgaatcgc ttgatccttc tttagcctta ctaggatcac
    16681 tctctttgct attttccata cttgattctg ttgttgactg attggtacga ttcaataatt
    16741 cttctaattg ttgttcatac tcttttcttg cttgttcttg ggcttcttcc ttgattgacg
    16801 tcgttgtggc cacagaatca gcctgtgaag attgattgat atgattaaaa gcaaatactg
    16861 ttcctccacc tagaagcaag cttaaacttg caatacatcc caccatccat ccgttaaagg
    16921 aacgctgtgg tgaaggagtt agatttgttt ggttgttcat tggggtttga acagcagcta
    16981 ctgaaggcgt tggttgaggt tgtgctagat aatttaactg agcttgtcgc aatgtatcca
    17041 tttgttcttg acgttcctta cgagcagctg cttctttttc cttttcttgg tttaaccgtt
    17101 gtttttctat tgccatttgt tgttccgcaa gatcctgttc acgttttttt agttctaatt
    17161 gttcgcgctc tttttctttt tgccgttgtt tttcttcttg ttctttcaat tcttgttgtt
    17221 gaatttcata ctcttttttc cattttggaa tttgcacttg taattcttgc tggatctttt
    17281 cgtgtttttc cataagaact tcagtcgttg tttgatatag cttattcagt tgttcattta
    17341 attctttgct aatctttagc ttatctgtac gcaaacctcg gtaaaattct tcttttcgtt
    17401 ctttaatacg ttcttttaac aagttttctt tttctttcga taacgcttct tgattcgtct
    17461 gagtaaaatt attgatcttt gctacacaca aggaatacgc attgtcgcgt ttcttttcgt
    17521 atcgctcttt aattttttgt acttcttctt caatcactcg ctgttcttct ttatttagtt
    17581 cctcttttgc tttctcccaa ttagacgact gttccgtcgc atactcatta atttcttgat
    17641 ctgtttcttg ttgacgttct tcaaaggtgg ctaataaatc ttcttgtgca agctcatgcc
    17701 aatttgttga ttggacagac tctaactgtt ctaaaagtgt gcctttaccc tgtgagatta
    17761 attcttcttt actctctaaa aatgcttgat aggtttgggg taattgatat ttcgaataaa
    17821 gctcatttaa aaataatttg tttttttctt tcatccattc atgctctggc ggatattgat
    17881 ccaaaatata atcaaataca gttgcctttt gtatctctat ttcgtttttt tctacttctt
    17941 cactgataag tggttcaata tattcctgag aatccccttc agtccatgtt gactcttcat
    18001 tagcttcaac tggatcaact tctacttcgg ccgtctcttc atcctccatt gtttcttcaa
    18061 agggatctac ctccaattga cccatcacat cgttctcttc ctcaaatatc gatttctgag
    18121 tcggaatatc agcttgttcg tcaacataga ctgtctcttt ttcttttgac tccttctttg
    18181 gaaatattgg tcgttcccat ggtacttctt catttacttc gacttcactc ccttgattca
    18241 cttttctttg gtcgctctct aactcttctt ctaaaacttg taaaagtttt tcgtttcgat
    18301 aagactcttc cacactcata gtttcatccg tttctttaaa cacatcaagg cggattaaat
    18361 ccatcaatgt aggatagaaa ccttctgaaa tttgataatc cagttgccca aaaacgtatt
    18421 ctgtaccagc tttctctatt tttccttctg tgtcatgtaa cgtgagagtc aacttaacag
    18481 aagatcgact ttcgtgttcc agtactttcc ctaacacatg tagctgttct tccgtttcaa
    18541 tgacagaata tttaattgtt ttcggtaaaa aagaagtacg ctttcctact tccttcgttg
    18601 ctttttttat taactcttta tctgttttgg tattgcctgc aatcgtaaag tacatgagta
    18661 ttctcctttc ataaaaaagg agtaaaaagt atcgactact tcttactcct acttttttac
    18721 tttcttataa tccttgaggt gtttgtccaa acaatccaaa taacgaaacc acaatagaag
    18781 ctgctgctaa aataactcct acccccatca atacatagcc tacgtgcatc ttcccatttt
    18841 cagctcctct tcgtccaaat aggaagaaaa gacccccaat aacaatggaa gcaacggccg
    18901 cagcagcagc aaaaatcatc agattgtttt ttaaactagt tgcggcagtt tgtaatgctc
    18961 taaaaatatc aaccgcagct actactttcg gttgtgttaa aaaattcatt gtaattgatt
    19021 caaacataaa attgttcgct ccttttagtg tttaataatt tcgtcgaaaa caagcttttc
    19081 acgttcacgt tgatagatat atccttctaa gtccttgtct tttccccgta ataggtcaaa
    19141 ttgtgctttt tcaaggactt cttgtttcca ctgtgtttca gatacaccgc ggcgttctaa
    19201 gattttagac gcggcttttt caaatggcgt tttagctttt gccactgtga ttcctccttc
    19261 aggacaaata aaaagactag caatcactcg ctagtctttt gtaacttata tttattggat
    19321 tgtgatccat cgtgaatcaa tttctctttc gttcgctttt catccttgct agatccaaga
    19381 aaaattattt tttttacgcc attcttctca tagggaacaa caataaataa agaatcagat
    19441 ttgtttataa attccctaac accagctgaa tctactattt tgtatctttc taaaccttta
    19501 tagtcttttt gcctcttgac tgttatttgt acaggatctg gatgccatac actctcagag
    19561 tattcccagg tagtctcgtc tataattttc aaattataaa caagaccacc tacttcttct
    19621 ttccacatag ttcctgattt caatacgcca ttatcttgat tagtttgttt atttttttca
    19681 ttactacatc cgactaaaat aacaatcatg gcaatcaagg aagtaactac tattattaac
    19741 tttttcattt tattcattct ttcgtttttt tattttaaat gtatctcaga accgcataac
    19801 taaaagttgc taacacaagt aatagtaagc tgaccgctga aatcgttact aaatgtgtag
    19861 aacctgtttt aggaacaatg acctgtggca agttaggatc aaccccaatt gtcacaacat
    19921 ttgttttatt agaaacatta tctactgcta ctacggcttg gttatcaata tttttttctg
    19981 attgtaatgg agttttagct gtaaatatca gctgaatttc tcgtccatct aaaagattgt
    20041 tacttaagaa gtcagcggaa gcttcccact tcagcgttct tgaattgcta tcataggtta
    20101 gtgtgccttc agaagtaata tcttttcctt tactcttatc tattacttgc gcagaacctt
    20161 ctttatatgt caaacgagct tcaattggat cttgaatact aaattgtttg tagtgatctg
    20221 acgcagcgtc tacgcctttt gtgtgccatt tttgtgtgac tagatattgt acttcagatc
    20281 ctgcgtttaa aggagctgtc ggcgtaccct tcttaccgtc atttcgtccg tttacatctt
    20341 ttccattttg ttgtacttct ttcactggaa ttttagggaa ataaacggta atgatatttg
    20401 tatccttttc tactttatta atgatcgacg ttccctgatt tttggctaca ctattttgat
    20461 tggccaattc attgactttt acattgatca ctaattcgta agtttcgccg ttatacttca
    20521 ttcgattttt taatgtatca gctttcgctg catattttac ctggtgtttt gttttgtcat
    20581 aggtcacttc accagataca tcgaattttt tatttgtacc actttcaacg tgtgcatcga
    20641 cgtaatttac ttcttttggt aaattatcga ttaactcaaa tcgatcgtaa accgctagta
    20701 aatccactcc taatcgattc accttctgtt tgattcgata ttgaatcgtg tctcctactt
    20761 gcaacatttt atcgttcacg ttatttccat ttttatcaac ccctgttttt tcaggttgat
    20821 caggaacaac actaaagatt gtcgcagaag ataaagtaaa ccaggtttga acattagaag
    20881 aaccaatcac aaacgtcgga tttgcttcag atattttaaa ggacacagta ccttgaccaa
    20941 acttcggatc tcctaattta tcatgcaaat tattgaaatc attatccgct tgtgtcagct
    21001 tagagcttcc tcctgggtgg ccacctacca cgttcagatt gttgtagaaa gatttaaatt
    21061 ctgaaagaac cgtatctttt ctgacatacc aatctgtcgt attcattttg tcatagtggg
    21121 catattctcc tttaagatcc gtggtttttc ctcctgcagg gttcaaagag ttaaaactga
    21181 taaatgtatc tcctgaaaat tgaacggggc taccgtcctc atatgtggct actagctgca
    21241 cattcatttg tgttgaatgg taaacaattc ctgagaaaag attgtctgag atccaaagta
    21301 ctgttctttt tttagattta ctcattgcac cattcgtatt tttatagcca tcgtctcgaa
    21361 aatgagtttt tgtatagttg ttatctagta gattccacgg caacgtatcc gagtataagt
    21421 gaatattaga tagaactagc ttgatattga cttttttccc gttatacgta ccgatttttc
    21481 catagtcaat attgactttg gtgttggttg ttacgccttc taaaacaagg tttgtttcac
    21541 cgtattcttt ttggaaagaa ggatagcgta tatttttgtt tccctccact aatcctacgt
    21601 agtcgtctgt aaaaccacct ttgttggcat caacatgata tttgtaaact tttgacgctc
    21661 cgtttacagt aatatttgtt tttgcattag gaacatcttt tggaataata aaattattct
    21721 ttacgttctg gattggattt gacggatcga caataacttt ttcagcaaag acattggctg
    21781 tttttaaccc aaacaatgta cctaacatca tcattatggg aaaaacatat tttaaccgtc
    21841 ttgtccacga gtgtttaaaa ttcatccctt catcccctct tttttataaa tgactagtga
    21901 aattccgaca ataacgatca aattgatcgt agtaaataat ggattcctaa tgagcttttc
    21961 cataatatct agcggcatgg aagcagctgg tatgtttaaa atcctcatta ggtatagttg
    22021 gtagcttggc aaaatagata gtttagaaaa taataaaccg cagtacaaaa agactgcggt
    22081 taaacaagaa aaattgaata ataataattt gatcagtttc attttatttc ttccttaatc
    22141 tctagaaaac aagtgagtaa caaaatccca acaattctca aagaagtttt tctttacgcc
    22201 agtagaaacg atttttttta cttcttcttt aggttgcgaa gcttctgatt taggatagat
    22261 ccacacatcc tttaagaatt caccgttttt atcagaaaca ggtgttaata gcaaaatatc
    22321 ctcgctatta gaaatgtttt caggacttga ttcattgaca atataataag cttgtttctg
    22381 atttgcctgt acttctatcg agaattcaaa gacacctgtt tgttgatcaa ttgtctttgt
    22441 ttttccatta gaaactagct tcaattgatc actgaatagt tttttcactc gatcttttaa
    22501 ttgggattca atttggtcag tagtgagatc cttaacatcc atcttttgta aaatgtccga
    22561 tacatcataa acagaaaact tcgcgccatt tactccttct tgtttttcaa gtttttgccc
    22621 ctggttgtct aattgttcgg aagaaataat tttttgaacg tgaatggtct gtttgactat
    22681 ttgatcgtct tcagcaattt tcggttcttc tgaagaatca actgttgacg aatcgacggt
    22741 ttcatcgctg ctttgaatcg tgctgctatc tgacgtttgg attgtttgtt ctgtgctatc
    22801 cgtgctttct tgtctgtctg caaacgtcaa ctgtgtgtag gcagtgaaag aaaaaagagc
    22861 ggctgcacaa acaaatagcc attttctatt tctcatgttg gttaccctcc tttatttttt
    22921 ctttttataa acaaatagcg aaccaatgat catgactgaa agaaaaacag tacttcctaa
    22981 aagccatgtc caagataatt tttgtttctt attttcttca tcttttaatt ctgattttgt
    23041 aaaggttact cgcttcccat acactagaaa acggtgtgtg ttgatcccct taggcgtaca
    23101 tgtcattagc gttactcgat cttcattggg atttggttct aagtaagtga caaagtttct
    23161 ttgtagttcg tctttttgca cttcctcaat ccgatcgatt tgataagcgt gctgctcacc
    23221 atcaactttg atataaaact tttctccctt ttttactgaa ggtaaatcat caaaaagatt
    23281 atctttataa agtccactat gacctgcgat taaaggattt ttaccgtttc ctcctgtaat
    23341 atcaccagtt ccttccgtaa tacctacacc attttctaaa attttgtcgg acgttccgtc
    23401 ataaattggt agttttaaag aaatacttgg aattgtaaga ataccaacag gatcgcctaa
    23461 ctcaatctta gcaacatcca aagaagaatc cgtttctgct gccgttcctg ttcgatcgat
    23521 tgcgacaatc tgctgatttt gttggtattt cgccacgctc gcttcagcag gcatcaaaag
    23581 taagacacct acagctgaaa gtaaccagaa agcaatgatc catttaccca tgattcgctt
    23641 tttcttaggc ttcctgataa tcattggttt tgatccttct aggctttctt cttaaagtaa
    23701 acaactgcag aaagtgcccc tgctcctagc aacaagcaag agattaatac atacgtttga
    23761 aaaccgttgc taccggtcat tggtacggaa gctttttgat tgtttttgat cgtgattcgg
    23821 ttttcttctt tgtatgaatc tttgtctact ttgaaagaga tctctttcgt taatttttga
    23881 tagttttcag gcgcttttgt ttcttccagt gaatattcac cttcagttaa accagtgatt
    23941 tcaaagtgac cttctttatc tgattttagg atcgctgcat ttgctacatc gtctacccat
    24001 actgcttctt tgacaccgtt tttatccgta tcaactaatc caccataata ttttccttct
    24061 gcgtttttag cagcaaattc tgcgccttca agcggtgttg ctttgattcc tattccaaat
    24121 agtccgctat cgacttttaa gaattttgca ccataagtca caacgtcagc tttcgttttt
    24181 tcgtggtgtt cttcctgatt aaatggattg ttgttataaa cgaaatccac gctatttgtt
    24241 aatggattgg caacagctgc gctatcattt acatacatgt tataagtcat ttgaagatct
    24301 tgtccgtgta aacttgctac agtatcactt acttggccat tattcacgat aaaatcgatc
    24361 acaaagccat tttctgaatt ttggatttta taatctgtat tttcttttaa taccacagat
    24421 ccatctttat ttgttacttt gatatttgct ttagcagcga cataagaaag tccttgtcct
    24481 gctatatccc cccatttaaa tacactgtaa ggagaagttt cgcttttcac tccgtttact
    24541 gtgataccgc ctgctccgcc tgcaatgtct gttgggatat gaatcgttcc ttgataattt
    24601 actgcgtcac caatttggaa agaatgttct tgtcctgttc catttgttac tgttttatcg
    24661 atcactggtg ctttatagtt tacgtatgaa gcttttaagt cacttgtcac gccatcattc
    24721 gtaatttcaa aggttaattt attgtgcgca tcgttgcgag cgtctgcgcc taataaatat
    24781 tgatctgctg ttggttcttt atctgatccc actacaactt ctgaaggaac ttccacaaaa
    24841 tagtattttc ctaacgtcaa atcagtagca gtcagctttc cttgatcatc cgatacaaga
    24901 tctcctaatt ttgttccttt tccaggttcg cccttgtata actcaaattt tgcccctttt
    24961 aaaggtgttt gtttgctttg agctgtgcca tcgtcaccaa atttcgttaa ttcaaaactc
    25021 aacttagaaa caatattttt aggatataag tgaatgtctt ttaaaaaacc tgaacctgta
    25081 ttatcagtca ttggcgcaat cacaaccatt ggttttgctt tttgtgtgac taatccagca
    25141 gcggatttac tttcataaac cacatataca ttccctttgg aattgacata ggctggttga
    25201 tcagcaaaag taatctcacc attttcatct acagctgaag taattttatt ggttgcattc
    25261 ttgacgtact cactgttaga tcccttgtct tctacatctt taacgatttc gtccactttc
    25321 gcgttggtta gatcagaatc ttcagtcggt aatacttgat ccgtaatatt tcctaacgta
    25381 aactccacat caccataatc tgccttgttg tatttcgtta cacctgtagg atattgatcg
    25441 tgcgtataac catcgttttt gatcccgtca atatcaacat tcaattgcgt tccttgatcg
    25501 tacattaatt tatgaaccgt gatattgacc ttctctggtg gcgttttttg tgtgacatct
    25561 tctgcatatg cttgataccc ctgaaagcag cttgcaatga ttcctaacat tccaacaagc
    25621 attccagctt tttttacata ccgatttatt gccatttttt tatctcctca tttcatattt
    25681 caacgactcg cgttgattaa aaccttgtta ttgtagctct ccctgtacca cgtatctttc
    25741 ttttccttgt gacgtataaa cacaagtaaa caacgttaat aagggtttct ctcctttctt
    25801 tggcaaagac aagaaagagt tctcttcagg aacgccatcg attaatttaa agtactcagg
    25861 tacgataaat ttacgcgtta ccttgtaacg atagacgcca tcgttactaa ctaagtccat
    25921 cacctcccct aatgacaact tttgaatatc agaaaataaa acacctggca tttccatgtt
    25981 atgacctgct aggacatagt ttcctttgcc catttcgcca tcttcataaa aataagtagc
    26041 cgctccaaga ctcaaagtgt attgatttgt ccctttaaaa attggcaatg ctaaaccaag
    26101 tgcaggaatc tgaatttgtg caaatacttg tttattgacg cccatttcga ttgcttcttt
    26161 ttgtatggac aacatttgac ccaaagacat atcattattg acgttcgttg catcctcctt
    26221 ataactcttt gcgtgtgcat tgatttgatc tacgggtatc tttttttcta tattttgctt
    26281 aaacacctct ccttcatgtt tttggtagct aacatagcca aacacactgc aaaagacgat
    26341 taaagcgact cctaaaaaaa aaataaccat tttttcatcc atctgcctcc tttcagataa
    26401 aacaaaaaac actttttcac aacgaaaaag tgttttctct tattactttc tatttcattt
    26461 atctggtggt tttatattgg tgtgtagcca ttgctaacat taagaataca attggcaccc
    26521 aactgattgt agttaatgtt tcttccatga atgattgaca catgatcgct aaaaaagccc
    26581 acaagaatgt tagttgtccg tttgtcagct cacattttct tacgagcaac cataaaaaac
    26641 taagcaaagg aataatcgat agagcaaaac caatcaatcc atagtgattg acgagtgcgt
    26701 gatacgtatt atggttacct agaatctgat ttcctatatg gaaaaagaaa ggtttcatcc
    26761 ctacaaatat ttgtcctgtt ttttcgccta atgtttcata gaatttgcgc caaatttctt
    26821 ctcgtcctgt aaataaatga actttgtctg acattgcagc aaaataagaa atcggtaaag
    26881 ctagaaccga aactgctgca tacatccaaa aggacaagtg tgtgtttcga aaaatcttct
    26941 taggtaaaat ttctaggata acgaagataa caagcgcgta taatgctgct ttcgattggc
    27001 aaacccaaat cgttgcccca gcaagaatca acgctggtaa tgatagcaat cgcatgtacc
    27061 attttacaaa tgaactagca aatcctgtga taagaagggc taatgtcatt aaagaactac
    27121 ctatcgtatt tgtattgatc caaatatctg ataatttgtt cgatggattc caaatacttt
    27181 gtccttctgg tactggtatc tcggtaaata ctcgataaac cgttacgagt aaaaagacat
    27241 agaatactac aagatacaag ctacgatcaa acacagtaaa ttccgtccat tttacaatca
    27301 aaataatggc aataattgtc ggaacgatca gatcaccata gaaagtatct actcggtagc
    27361 ttctaaaaaa agaaaagaaa aggtaaaaaa gaatgcctgc tgcaatgaag aataaatcgt
    27421 ttctctttac cttgtacaga ttcaaaagaa acaaacagcc tacgaaaaac gtcactaact
    27481 gtgcagcatt atcataaata atatcgtagg
//
LOCUS       DACGLE010000034        25877 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0534
            SAMEA3304449-rid9560453.denovo.035, whole genome shotgun sequence.
ACCESSION   DACGLE010000034 DACGLE010000000
VERSION     DACGLE010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304449
            Sequence Read Archive: ERR985064
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25877)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25877)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702225.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702225.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 20:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,045
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 27 of 67
            Pseudo Genes (incomplete)         :: 25 of 67
            Pseudo Genes (internal stop)      :: 19 of 67
            Pseudo Genes (multiple problems)  :: 4 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25877
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304449-rid9560453.denovo.035"
                     /strain="VREN0534"
                     /db_xref="taxon:1352"
     gene            complement(<1..701)
                     /locus_tag="IXT20_001537"
     CDS             complement(<1..701)
                     /locus_tag="IXT20_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HAQ4859986.1"
                     /translation="MRYSTILISTFAALSLSLTINQTVHTEEIETAKWTETKWANVNR
                     ITFQDEFSSFNSGVAKLRKGQTATIKAKIDYSGDLAPILQSAVTFENVDPALSIGYDE
                     NTLTIQHKQAIFTFTVTLNQDLLKPALFTVKVSDGLPNDHHHLTPYSQRQTIEQDSAI
                     DSGGDLVEEPTDKPENENKPEVPPTENPDGEQKPEIEPGEEPDTETQPEPDNESKPEI
                     TPGEKPDVDPEEKPDV"
     gene            849..2402
                     /locus_tag="IXT20_001538"
     CDS             849..2402
                     /locus_tag="IXT20_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAQ4859987.1"
                     /translation="MQTQEAIKTFLLKKKSNTKLDIFLFLSEHRFFITTQYLADHFHM
                     SESNFLLYIKELEQDFERLNLTELHIDKQKPFLKLNFEGIDPAYCYYRLFGRYCNESV
                     SYQILTSLFSCQTNSIISLSQQTNYSASYLYTKMKKINAFLALYGLSISFSNNGRKSF
                     SGKEVQIQYAALDIYWNIFSSTATEFYDEDPQVVAFMMNTFFKKEVLDRLNSGMIDKL
                     YFLLKICKENFPATSVKDVQKEFTEYEYIDFFIDPSVDILRPNLPICREQRILVNILA
                     RLSITKIESEETSLEQYKLLKKANVPHVLYSREMVESFCTVFDLFIPEKEKILYSLAF
                     ARNKLYNGFLNFNQPNTPLPTFMLYQEHSVLKEVQTAIEQFYMDFREQNQHLLPVILE
                     KENVQWMVEDLVHLYDRYKKQPRIVIGVNYTRDFYISKDLIVKIEQIFSNESIKIQND
                     FMENCNIVISDCPLKQLPSHIKKIYILKGIITPEDWKKVIMRISEYIFELKEEALMDQ
                     SKKENQVTS"
     gene            complement(2510..3592)
                     /gene="uxuA"
                     /locus_tag="IXT20_001539"
     CDS             complement(2510..3592)
                     /gene="uxuA"
                     /locus_tag="IXT20_001539"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HAQ4859988.1"
                     /translation="MEMTFRWYGSQEEKITLDEIRQIPAVKGIVGTLMDIPVGEVWPR
                     ERIKELKEEIESRGLTLKVIESVNVHDAIKIGLPSREQYIENYKQTIRNLAEYGIEVI
                     CYNFMPVFDWVKSDLDYRLEDGSSTLAFISDDIPDDPREIVARIEHSSNEFELPGWEP
                     ERLAHIKSLLEAYASVDEEKLRENFAYFLQSIIPTCEEVGVKMAVHPDDPPYSIFGLP
                     RIVKNREDLDWLCRIVDSPYNGITLCTGSIAEDPENDVYGILAEFCQRDRIPFAHVRN
                     IKFLANRDFYEAPHKSEYGSLDMYRILKTMYDNGFNEYIRPDHGRMIWNETGRPGYGL
                     YDRALGAVYLNGLWEAIEKGNKSKDN"
     gene            complement(3583..5229)
                     /locus_tag="IXT20_001540"
     CDS             complement(3583..5229)
                     /locus_tag="IXT20_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HAQ4859989.1"
                     /translation="MIDLNNWRNFIPELSQKEVEIPHFDRASLRENGQKNPVWIHFGG
                     GNLYRGFHAEIAQRLADLGELSTGVIVCETFDEEIIERAYQDFDDLILEVVMPSSGEF
                     VKRILAATADSLYCHPDCSEHFDKMIRHFENPSLQLVTFAITEKGYRLKDTNNKYIGP
                     IAVDIKNGPKKPSHTMSILAALLYKRFLSGAWPIALVSTDNFSQNGSQLRTSLLTIAK
                     EWQKEGYVQNTFIQYLSNEEIVAFPWTMIDRITPNPSLSVSKMLAKEGFSNMSIFQTA
                     KGNNIAPFVNTEDIHYLIMEDHFPNGRPKFEKAGVFLTDRATVDKADTMKVTTCLNPL
                     HTSLAIMGCLLGYRSIAEEMKDPDLSLLVSEIGYNEGLPVVESPGIIDPKIFLEEVIT
                     VRFSNPNIPDMPQRIATDTSQKVAIRYGETIKKYMEIEVKDPQTLVFIPLTLAAWLRY
                     LIGIDDQGKPFIPSPDPLLTDFQHKLSDIQLGTQDTSHIHEKMAPILSNKEIFGVDLY
                     QAELGRKIEEMFIKMVAGPNAVRKTLQDYLQEHGGNLSWK"
     gene            5413..6075
                     /locus_tag="IXT20_001541"
     CDS             5413..6075
                     /locus_tag="IXT20_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ4859990.1"
                     /translation="MKSSLQSQAYQAIRKQIIYADLEPGRKISEKALEEHLAIGRTPI
                     REALIQLRQQELVYTIPQSGTYVSKINLVSAENARFVREQLEEQIMIECCSKLDTQTR
                     IVLETILEEQEKAVKINDERAFFRADNLFHEACFEIAGRKEVWNWLDDHNTHLERFRW
                     LRVTIQGLPWETIMEQHYDLLKALVDKKPEEARFQTGLHLHMMLKEQETVTQRYPEYF
                     QQ"
     gene            6219..6782
                     /gene="ahpC"
                     /locus_tag="IXT20_001542"
     CDS             6219..6782
                     /gene="ahpC"
                     /locus_tag="IXT20_001542"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003756453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HAQ4859991.1"
                     /translation="MSLIGKKIEEFNAHAYHQGEFLEVSEKDLMGNWSILCFYPADFT
                     FVCPTELEDLQEQYSTLKSLGVEVFSCSTDTHFTHKAWHDTSDAIGKIEYVMIGDPSH
                     QISRIFDVLDEEQGLAQRGTFIIDPDGIVQAMEINADGIGRDASALIDKIRAAQYVRT
                     HPGEVCPAKWKESGETLKPSFDLVGKI"
     gene            6792..8321
                     /gene="ahpF"
                     /locus_tag="IXT20_001543"
     CDS             6792..8321
                     /gene="ahpF"
                     /locus_tag="IXT20_001543"
                     /EC_number="1.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HAQ4859992.1"
                     /translation="MLDKETKQNLEQYLALIESPIVFSVSLDNSENSQKLAEFTKEIA
                     EMSPKISWEKRDSMRTPSFSINPVGKESGIVFAGIPLGHEFSSFLLAMLQISGRAPKI
                     SESLSLKIKKITQTLHFDSYVSLTCHNCPDVVQALNILAVLNPNITHTMIEGGMFQKE
                     IDDKKIMAVPTVFLNGEEFSSGRMTIEQIVEKITGPLIEEELFEKEPYDVLVIGGGPA
                     ASSAAIYAARKGIRTGLVADSFGGQVVETLGIENMIGTSYTEGPKLMQQVESHVRSYP
                     IDIMMNQQAVSLNKEQHFINIGLANGISLKAKTAIIATGAHWRSINVPGEKEFKNKGI
                     AYCPHCDGPLFKGKEIAVIGGGNSGVEAAIDLAGLAKHVYVLEFLPELKADQILQDKL
                     VSLANVTVIKHAATKEISGTNQVESLSYIDRRTMEMHTLAVSGVFILVGLLPNTDWLD
                     GTIEMNPRNEIVTEKNGATNIPGVFAAGDCTDSPYKQIIVSMGSGATAALGAFDYLMR
                     T"
     gene            8478..8828
                     /locus_tag="IXT20_001544"
     CDS             8478..8828
                     /locus_tag="IXT20_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2200 domain-containing protein"
                     /protein_id="HAQ4859993.1"
                     /translation="MAQNRIFTTSFASVYPMYVAKAERKDRIKEEVDEIICWLTGYSQ
                     TELEYQLQKQVTFEQFFAEAPVPNPSRSLITGVVCGVRVEDIEDNLMKEIRYLDKMID
                     ELAKGKKMEKILRS"
     gene            8927..9457
                     /locus_tag="IXT20_001545"
     CDS             8927..9457
                     /locus_tag="IXT20_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4859994.1"
                     /translation="MFETIKQNIQKYALLRAAVYIISGIAIVLNPSAVFHFIGYLITA
                     YLVLLGLLNLFEAYRFKRKTGSWSIGLFSGLFFLILALIVYAFAPAIVSILPILLGLA
                     VIINSLFQLFFSMNTKAKSWSVYSSILLLIGGFVLLFNPFKSLMLLFQIFGFILIFMG
                     IQEIINFIRIRKMTIR"
     gene            9611..10762
                     /locus_tag="IXT20_001546"
     CDS             9611..10762
                     /locus_tag="IXT20_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HAQ4859995.1"
                     /translation="MEFIGILCLILISTTIGSHISRRLGIPAVIGQLLVGVLLGQAGL
                     NWVKPDILVHDFSEIGVFLLMFLAGLESDLSLLKKYFRPGMFVALLGIIFPVVFGWLT
                     GKAFQVSGNEAIFFGIILAATSVSISVEVLKELNVVNTKEGSTILGASVVDDILVVLV
                     LSFSLSFLTGEASSNASLPLVLIEQLIYFVFIFLLVKWIAPFLLSFAEKVYANSSIII
                     VSLVICLGMSYVADLVGLSSVIGAFFAGIAVSQTKVKEEVYHNVEALGYAVFIPVFFV
                     SVGLEVDFSDFSEQLLFISILTLVAILTKLLGGYFGAQIAGFSQHSSLMVGAGMISRG
                     EMALIILQIGQQSQLIEPQYYSPLVIVILLSTLISPLVLKYFTKKVYEV"
     gene            complement(10871..12910)
                     /locus_tag="IXT20_001547"
     CDS             complement(10871..12910)
                     /locus_tag="IXT20_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HAQ4859996.1"
                     /translation="MKRSDKHGKNRTGAYIAGAVILIAAAGGGYFYYQHYQETQAVEA
                     GEKTVEQFVQALNKGDYNKAAEMTSKKAANKSALSEKEILDQYQNIYGAADVKGLQIS
                     NLKVDKKDDSTYSFSYKAKMNTSLGELKDLSYKGTLDRNDGQTTINWQPNLVFPEMEG
                     NDKVSLTTQEAARGNIIDRNGEPLATTGKLKQLGVVPSKLGDGGEKTANIKAIASSFD
                     LTEDAINQAISQSWVQPDYFVPLKIIDGATPELPAGATIQEVDGRYYPLGEAAAQLIG
                     YVGDITAEDIDKNPELSSNGKIGRSGLEMAFDKDLRGTTGVKLSITDADGVEKKVLIE
                     HEVQNGKDIKLTIDAKAQKTAFDSLGGKAGSTVATTPKTGDLLALASSPSYDPNKMTN
                     GISQEDYKAYEENPEHPFISRFATGYAPGSTFKIITAAIGLDNGTIDPNEVLTINGLK
                     WQKDSSWGSFKTTRVSSDVSQVDLKTALIYSDNIYAAQETLKMGEKKFRTGLDKFIFG
                     EDLDLPISMNPAQISNEDSFNSDILLADTGYGQGELLITPIQQAAMYSVFTNNGTLVY
                     PKLIADKETKDKKNVISETAVQTIVPDLREVVQDVNGTAHSLSALGIPLAAKTGTAEI
                     KEKQDVKGQENSFLFAFNLDNQGYMMVSMLENKEDDDSATKRASELLQYLNQNYQ"
     gene            complement(12968..13525)
                     /locus_tag="IXT20_001548"
                     /pseudo
     CDS             complement(12968..13525)
                     /locus_tag="IXT20_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303250.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
     gene            complement(14064..14270)
                     /locus_tag="IXT20_001549"
     CDS             complement(14064..14270)
                     /locus_tag="IXT20_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4859997.1"
                     /translation="MLKISYKPSTDSKEMKKEYETVNDFLQGQYLEVPPLQDHFVVTT
                     VTLDGKEIEMPDQTISGLFNYFNK"
     gene            complement(14286..14900)
                     /locus_tag="IXT20_001550"
     CDS             complement(14286..14900)
                     /locus_tag="IXT20_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HAQ4859998.1"
                     /translation="MFERSPSELTERENYKLLIGSVIPRPVAVVTTKSAKDIVNIAPF
                     SYFNIVSSNRPILSLAIQRKQDAVKDTAKNILETREAVIHILDEDNVEEVNKTAANLP
                     NEESELYLTDLTLTDSVIISVPALTEAKVRFETSLYEHVEIRNKNKVTADLFLLEVQK
                     YHLNETVYDDKTGRIDPRKLNAVSRLAGNDYAAIGEIFTIARPE"
     gene            complement(15047..15730)
                     /locus_tag="IXT20_001551"
     CDS             complement(15047..15730)
                     /locus_tag="IXT20_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HAQ4859999.1"
                     /translation="MDTLEQIKGGVVVSCQALENEPLHSSFIMGRMALAAKEGGAVGI
                     RANTTEDINEIKRIVDLPVIGIIKRDYDGSEVFITATEKEVEELLETQAEIIALDATS
                     RKRPTEKTTAELVQMIHEHGRLAMADISTFEEALQAQEDGFDLLSTTLAGYTEYSRKT
                     QTPDFDLLNQIIEQVDIPVIMEGHTDSPEQVEKAYELGAFSVVVGSIITRPQLITKRY
                     VEAASKANN"
     gene            complement(15839..16297)
                     /locus_tag="IXT20_001552"
     CDS             complement(15839..16297)
                     /locus_tag="IXT20_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HAQ4860000.1"
                     /translation="MYQQVEEYVKKFQEKYPSYQSEKLGFFSFGDTEKMADELSELVV
                     KKIKTGTSSGYDLYKQENEPLPKVGQLDVVLNGKNEPVCIIKNINVTILPFSEVTKEH
                     AWKEGEGDRTLAYWRKVHAEFFKHAYKEALNIEFTEEKLVVYEEFEVIFF"
     gene            complement(16381..18144)
                     /locus_tag="IXT20_001553"
     CDS             complement(16381..18144)
                     /locus_tag="IXT20_001553"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HAQ4860001.1"
                     /translation="MSTVLHVLDAINSPADVKKLSVEEMKQLAEEMRQKILQKDSDVG
                     GHVGPNLGVVELTIAFHYVFDSPKDKIVWDVSHQSYGHKLLTGRKNGFEKGHYREVSG
                     YSNQHESKHDYFTVGHTSTSISLATGLAKARDLKKESGNVVAFIGDGSLSGGLAFEGL
                     NNGGVLQSNLIVLVNDNEMSIDENHGSLYKNLAELRQTNGKSSNNLFKAMGFDYRYVD
                     NGNDLETVIRLFEEVKDIDHPIVLHVHTEKGRGYQPAIDNKMKYHWHVPFDLSTGEAK
                     QVTSGETFNMIIMDYLDKKMQTSTAPIAAVNAAIPGAFGLKTFQEKHPDHYFDVGIAE
                     QHSITFASALASQGVRPVVFQSSTFLQRAYDQLSHDLAINENPVIILVGGGTISASDR
                     THQGTFDIPYLSSIPNIKYLAPATKEDMISMLDWAFEQTAQPVAIRIPTHGVEHGTAI
                     ANDYSIPAYQTVNNGGKVAILALGGFLELGKETVKAFRKKGIEATLINPGTITENDVE
                     TLDKLKETHELVITLEDGSLSGGFGENISRYYGDSSMNVLNFGAEKEFTDSVPVGQLY
                     ERYHLTPKQIVEDSLRVLNIQ"
     gene            complement(18365..19825)
                     /locus_tag="IXT20_001554"
     CDS             complement(18365..19825)
                     /locus_tag="IXT20_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4860002.1"
                     /translation="MLENYIERNIFRKVYLCEQLFEFQEIDIEQTAISLRVTTPTILH
                     DLESLAECLEYCIKEQVREKHKYKLVFKHGIALSELTQFLYGQSYFLKFLSYYFNGIF
                     TSTELADLEFISLSKVYTIKKIVLDFFKENDYLKNKEIIIPEFDSRNILLALVRYVNW
                     EGYENKNPYVEEACNELIDYVETHFFKRRYSEDEKSFIIRGIEIAIGRKAFPLGLKEE
                     DKKSAETKPLFQLVCQGLEEQKGILDLQEEDVYYIFSLFNSRNYTNENMELLRKDFEV
                     VYKNFVLNDLSLNELILQIISIAEIDDAENFIFKQSFLQFVRTLWADGQVFLPERIYL
                     LSEKEQLLYGKIVEILEEWKKKNQIQLRWNENFIRKFVKSLSLINSEQAGRQEIEIFV
                     VSESSVKQFFYRTQFHLYAEEGIAEINPILFRSLEELPDECLCAKKRVVLCDAASYQN
                     GLETEKTKIYPISLKGANIHLYHIAQELHLLKKHIS"
     gene            complement(20033..20959)
                     /gene="glsA"
                     /locus_tag="IXT20_001555"
     CDS             complement(20033..20959)
                     /gene="glsA"
                     /locus_tag="IXT20_001555"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HAQ4860003.1"
                     /translation="MEVKELEQLIGKNFHYYQTGQVADYIPALAEVDPKQLGMAIYDL
                     DTKQLIEGGDADVRFAIESMSKVPVLLLAIKDNGIEKVFQTINTEPTGFAFNSIMNME
                     INHRKHPMNPFVNAGAIATTSLIAGKDADEKFERILSFMKEICDDPNITLNEEIYHSE
                     SRTGDINRSLAYYMKGNQMIEGDVPEILDAYFRQCSLNVTATGIAKLAAVLANKGIAP
                     WNGKQIITEESATIVKSIMTTAGLYDESGEFSVHVGVPAKSGVGGGLMAAVPNRYGIG
                     VFSPALDPFGNSAAGIQLLKDVVKELDADIFE"
     gene            complement(20982..22409)
                     /locus_tag="IXT20_001556"
     CDS             complement(20982..22409)
                     /locus_tag="IXT20_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ4860004.1"
                     /translation="MQQSKTKIPAKKLTFFGFLAMTISMVVSLYEYPTFATSGFSLVF
                     FLLLGGLLWFIPVALCAAEMATVKGWEKGGVYTWVSRTLGKRFGFAAIFFQWFEITVG
                     YLTMLYFLTGALSYATGIGAIQNNKYLKLAILLIIFWAILLSQLKGTKYTSLIARIGF
                     VAGILLPALVLFGLGIHYVASGAPLQTILSMKTLIPDFSKLPTLVVFVSFILAYMGVE
                     TSASHANEMENPKKDYPLAMFVLVILAIILDTFGGLTVATTVPQHGLSLNTGVIQSLE
                     FLLRHIDPNLAWIAKILAILVCLGVIGEIASWVTSPSKALHVAAEEGLLPEYFAKENT
                     HGVPAHLMIANGIVATIWAAVFTLSGGGNNLSFLAAMSLTVVIYLMLYFMFFAGYLKL
                     VFKDDSLERAYQVPGGKVVKVIVACLGLLTSLFAFCISFVPPASIAAGQHGIYETILI
                     VSYLIVLVIPFTIYACRHKFQHAEK"
     gene            complement(22440..23654)
                     /locus_tag="IXT20_001557"
     CDS             complement(22440..23654)
                     /locus_tag="IXT20_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HAQ4860005.1"
                     /translation="MNTLPINTGDTAFMILCTAMVCLMTPGLAFFYGGLARKNNILTI
                     MSQSFLSVGITTIMWVFGGFGLAFGRDIGGVIGNPADFFLMRHVTSFPNMFHGATIPF
                     LMFFLYQLMFIVITVPLMTGAFAGRLNLKGYIILVIFWNLLIYFPVCHWIWGGGFLDQ
                     MGFRDFAGGAVIHTTAGFGSLACILTLGQRKIPLGERSQHSSLMAAAIGTGLLWFGWF
                     GFNSGGALRADEQAVNAFGSTFIALAFAMITWLIIAKVKGNGFDFVDVLTGSVAGLAA
                     ITPCAGYVEAKSAMLIGIIAGIVCHAAVDFRKKKQWDDALDVWGVHGMGGFTGTILIG
                     IFASNSHLLTNPSSWYFLGIQVIGVIITAVYAYVLTTVILKSAMHFTTITTTKEEQEE
                     GLDFFFFGNKLN"
     gene            complement(23995..24237)
                     /locus_tag="IXT20_001558"
     CDS             complement(23995..24237)
                     /locus_tag="IXT20_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAQ4860006.1"
                     /translation="MGFIWALIVGGIIGAIAGAITNKGSSMGIIANIIAGLVGSAIGQ
                     ALLGTWGPTIAGMAIIPSLIGAIILVLVVSFFARKL"
     gene            complement(24269..24826)
                     /locus_tag="IXT20_001559"
     CDS             complement(24269..24826)
                     /locus_tag="IXT20_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HAQ4860007.1"
                     /translation="MENKNMNTTNGSVLANEKGENRMMNGELTYEDKVIQKIIGYALK
                     DVDGLLTLDGGFFSNMAEKLVNTDNVTAGINTEVGKKQVAVDLDVIVEYGKDIEDIYN
                     QIKELISTEVNNMTHLDVIEVNVNVADIKTQEEYQKDQETVQDKVTEAAKSTGQFASR
                     QTDKAKSAVGKGAQKVKENNEPRVQ"
     gene            complement(24839..25027)
                     /locus_tag="IXT20_001560"
     CDS             complement(24839..25027)
                     /locus_tag="IXT20_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HAQ4860008.1"
                     /translation="MKELFSAYKLPIIFGGLGLLLAVLWLTIGFFKTLLIIIMTAIGA
                     GIGFYLKQSGIVDQFFKS"
     gene            complement(25040..25606)
                     /gene="amaP"
                     /locus_tag="IXT20_001561"
     CDS             complement(25040..25606)
                     /gene="amaP"
                     /locus_tag="IXT20_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HAQ4860009.1"
                     /translation="MNKGLKIIGVIMSLLLLSVFLAIALINAPYIMPEQLERFRFFTL
                     TNYYMQQYIFWAAVVFAVLAIIILLVVLFYPKSRGTFVMKREDGKLTIDKKAIEGLVR
                     SHLHEEEFIHSPKVRIRSTKNRIHIHVNGDLKRTSSLVGKTGVLMQDIEEEVTKVLGT
                     KETVKVAVTYSGYEEQEDTRDYKHSRVE"
BASE COUNT         7585 a         5042 c         4519 g         8731 t
ORIGIN      
        1 acatctggtt tttcttctgg gtctacatct ggtttttctc caggggttat ttcaggtttt
       61 gattcattgt ctggctctgg ttgagtttct gtatcgggtt cttctccagg ttctatttcc
      121 ggtttctgtt ctccgtctgg attttctgta ggcggtactt ctggcttatt ttcattttct
      181 ggtttatctg ttggttcttc aacaagatct cctcctgagt cgatagctga atcttgctca
      241 attgtctgcc gctgactata cggcgttaaa tgatgatgat cgttcggtag tccatctgat
      301 accttgacag taaaaagcgc tggttttaac aagtcctgat ttaatgtaac ggtgaaggta
      361 aatatcgctt gcttgtgttg gatagtcaaa gtgttctcgt catagccaat cgacaaagcg
      421 ggatcaacat tctcaaatgt aacagctgat tgtagtatag gtgctaagtc tcctgaatag
      481 tcaatttttg ctttgatggt cgctgtttgt ccttttctta attttgcgac tcctgaattg
      541 aaggatgaaa attcatcctg aaaggtaatt cgattgacgt tggcccactt ggtttctgtc
      601 catttggctg tttctatctc ctctgtgtgg actgtctgat ttatagtaag cgataagctt
      661 aaggctgcaa atgttgatat taagatggtt gaatatctca taactctctc actccttgat
      721 ttttttgtat gcaagaaata agatagtaga gattctacct atatgtttac tgtttttttg
      781 tctaaattta tgaaaaaaac agtaatattt tcaagtttta acaatttaag taaaaaggag
      841 cctctttcat gcaaactcaa gaagcgatta aaacgtttct cttgaagaaa aaaagcaata
      901 caaaattaga tatttttctt ttcctatctg aacatcgttt ctttattact acacaatatt
      961 tagcagatca tttccatatg tctgaatcaa attttttact atacatcaaa gaattagaac
     1021 aagactttga gcgtctcaat ctaacagaac tgcatattga taagcaaaaa ccttttttaa
     1081 agttgaattt tgaaggaatt gatccggctt actgctatta tcgtctattt ggaagatact
     1141 gcaacgaatc agtcagttat cagatcttga cttctttgtt cagctgtcag acaaattcga
     1201 ttatcagttt aagtcagcaa acaaactata gtgcctctta tttgtatacg aaaatgaaaa
     1261 agattaatgc atttttagca ttgtacggtt tgtctatctc cttttctaat aatggcagaa
     1321 agtctttttc tggaaaagag gtacaaattc aatatgcagc attagatatt tattggaaca
     1381 tcttttcaag tactgctaca gaattttatg atgaagatcc gcaagtagtg gcttttatga
     1441 tgaatacttt ttttaaaaaa gaagtacttg accgactaaa tagcggtatg atagataaat
     1501 tatattttct attaaaaata tgtaaagaga attttcctgc aacttctgtg aaagacgtac
     1561 aaaaagaatt cacagagtat gaatatattg acttttttat tgatccctct gtagatattt
     1621 tgcgacctaa tctgccaatt tgtcgcgaac aacgcatctt agtgaatatt ttagctcgac
     1681 tttctatcac gaaaatcgaa tcggaagaaa caagcttgga gcaatacaaa cttttaaaaa
     1741 aagcaaacgt tcctcatgtt ctttattcaa gagaaatggt ggagtccttt tgtactgtat
     1801 ttgatttgtt tatacctgaa aaagaaaaga ttctttactc attggcattc gcacgaaata
     1861 aactttataa tggatttctt aacttcaatc aaccaaatac gccccttcct actttcatgc
     1921 tttatcaaga acatagcgtg ttgaaagaag tccaaaccgc cattgaacaa ttttatatgg
     1981 atttcagaga acaaaatcaa cacttgttac cagttatttt agaaaaggaa aatgtgcaat
     2041 ggatggttga agacctagta catttgtatg atcgctacaa aaaacagccg cgaattgtga
     2101 ttggtgtgaa ctatacgcgt gatttttata tcagtaaaga tctgatcgtt aagatagaac
     2161 aaattttttc aaatgaatcg atcaaaatac aaaatgattt tatggagaac tgcaacatcg
     2221 ttatcagcga ctgccctttg aaacaattgc cttctcatat caaaaaaatc tatattttaa
     2281 aaggaatcat cacaccagaa gactggaaaa aagtgattat gaggatctca gaatatatct
     2341 ttgaattaaa agaagaagca ctcatggatc aatcgaaaaa agaaaatcaa gttacttcct
     2401 agaccaaata agatacataa atccgaatgc ttattaacaa cctccggatt tatgtatcaa
     2461 gaatttgaac atccttctta tttacataat aaaattacag tttttttcac taattatctt
     2521 tacttttgtt tcctttttcg attgcttccc ataatccatt tagataaaca gcacctaatg
     2581 ctcgatcata taagccgtat cctggtcttc ctgtctcatt ccagatcatt cttccatgat
     2641 caggacggat gtattcgtta aatccgttgt catacattgt ttttagtatg cggtacatgt
     2701 ctaatgaacc gtattcggat ttatgcggtg cctcatagaa atctcgatta gctaaaaatt
     2761 taatatttcg aacatgtgca aatgggattc gatctctttg acaaaattct gctaaaatcc
     2821 catatacatc attttctgga tcttctgcaa tactccctgt acaaagagtg attccgttat
     2881 aagggctgtc tactatgcgg cacaaccaat ctaaatcttc tcgattcttt acgattcgag
     2941 gtaatccaaa aatcgagtat ggcggatcat ctggatgtac agccattttg acccctacct
     3001 cttcacatgt tgggatgatt gattggagaa aataggcaaa attttctcga agtttttctt
     3061 cgtctactga tgcataggct tcaagcaagg atttgatatg agctaagcgc tcaggttccc
     3121 atcccggcaa ttcaaattca ttgctggaat gttcgatgcg cgcgacgatt tctcttggat
     3181 catctggaat atcatcagat ataaaagcta acgtggatga gccgtcttct aagcggtagt
     3241 caaggtcaga tttcacccag tcaaatacag gcataaaatt atagcagatg acttctattc
     3301 catattctgc caagtttcgg attgtctgct tatagttttc gatatactgc tcacgggaag
     3361 gtaatccaat tttaatggca tcatgcacat tgacgctttc aattactttt agtgttaacc
     3421 cacgagactc aatttcttct tttagttctt tgatcctctc cctgggccat acctctccta
     3481 ctggtatatc catcaatgtg cctacaatcc ctttgaccgc aggaatctgt ctgatttcat
     3541 ctaaggtaat cttttcttcc tgtgatccgt accatctaaa agtcatttcc atgataaatt
     3601 tcctccatgt tcttgtaagt aatcctgtaa tgtttttcgt acggcattcg gtcctgctac
     3661 catcttgatg aacatttctt ctatttttct gccaagctct gcttggtaaa gatctacacc
     3721 aaaaatttct ttatttgaca aaataggagc cattttctca tgaatatgtg atgtatcttg
     3781 cgtacccagt tgaatatcgg aaagcttatg ctgaaagtct gtcaatagtg ggtctggact
     3841 cgggatgaat ggttttcctt gatcatcaat gccaataagg tatcgaagcc aagcagcgag
     3901 agtcaatggt atgaatacaa gtgtctgcgg gtctttaact tctatttcca tatatttttt
     3961 aatcgtctca ccataacgaa tcgccacctt ttgagaggta tccgtggcga ttcgctgagg
     4021 catatcagga atatttggat tagaaaagcg cacagtaatt acttcttcca gaaagatctt
     4081 tggatcaatg atacctggag actccacaac tgggaggcct tcattgtacc cgatttcaga
     4141 aactagcagc gataaatctg gatctttcat ctcctcagct atgctcctat accccagtaa
     4201 acaacccata atagctaaag acgtatgcaa aggattaagg caagtcgtca ccttcatcgt
     4261 atctgcttta tctaccgttg cacgatcagt caagaatacc ccagcttttt cgaatttcgg
     4321 acgtccattg ggaaaatgat cttccatgat taaataatgg atgtcctctg tattgacaaa
     4381 tggagcaata ttatttcctt ttgcggtttg gaaaatcgac atatttgaaa agccctcttt
     4441 tgccaacatt ttgctaactg acaaggaagg attcggggtg atacgatcaa tcatcgtcca
     4501 tggaaacgca acaatttctt cattcgataa gtattggata aatgtatttt gtacataacc
     4561 ttccttttgc cattcttttg cgatcgttag caaagaagta cgtaattgac taccgttttg
     4621 tgaaaaatta tctgtagaga caagtgcaat cggccaagct ccagataaaa aacgtttata
     4681 aagtagcgca gcaagaatac tcattgtatg tgatggcttc tttggaccat tttttatatc
     4741 gactgcaatc ggtcctatat atttattatt tgtatctttc aatcgataac ctttttcagt
     4801 aatagcaaaa gtaactaatt gtaaactagg attttcaaag tgtcgaatca tcttatcaaa
     4861 gtgttcagaa caatctggat gacaatacag actgtcagct gtcgcagcta atatacgttt
     4921 gacgaactct ccactgcttg gcatgaccac ttcaagaatc aaatcatcaa aatcttggta
     4981 agccctttca attatttctt catcgaatgt ttcacaaaca atgacaccag tggacaattc
     5041 gcctagatcc gctaaacgtt gagcgatttc tgcatgaaat cctcgataaa gatttccgcc
     5101 accgaaatgg atccagacag gatttttttg tccattttct cttaatgatg cacggtcaaa
     5161 atgaggtatt tctacttctt tttgagataa ttcagggata aagttcctcc agttgtttaa
     5221 atcaatcatt tttcccctcc taaactaaca tattagtttt tgtattcaag tgaattttat
     5281 acaataaaaa tcgctatgtc aaccaaaatt ttactattta tacaaattta ctagttaatg
     5341 tgacatacca gattgttttt cagacctctt ctgttatact acgttcatag aaatgatttt
     5401 ggaggaataa agatgaaaag cagcttgcaa tcacaagctt atcaagcgat tcgtaaacaa
     5461 attatctatg ctgacttaga acctggaagg aaaatatctg aaaaggcact ggaggaacat
     5521 cttgctatcg gccggactcc cattcgcgaa gcattgattc aactacgtca gcaagaactc
     5581 gtgtatacga tacctcaaag tggtacgtat gtttccaaaa ttaatcttgt ttctgcagaa
     5641 aatgctcgct ttgtccgaga acagttggaa gagcaaatca tgattgaatg ttgttccaaa
     5701 ttggacacac aaacacgaat agtgctagaa acgattttag aagaacaaga aaaagcagtc
     5761 aaaataaatg acgaacgcgc ttttttccgt gcagataatt tatttcatga agcttgtttc
     5821 gaaattgcag gaagaaaaga agtttggaac tggttagatg accataatac ccatttagaa
     5881 cgtttccgct ggttacgagt gaccattcag gggcttccat gggaaacgat catggaacaa
     5941 cattatgatt tactcaaggc tttagttgat aaaaaaccag aagaagctcg atttcaaacg
     6001 ggattgcact tacacatgat gctgaaagaa caggaaactg ttactcaacg ttatcccgag
     6061 tatttccagc agtagcattt tcttgcgacc tcttctattt ttataattaa ccttcgtttt
     6121 tgagtaatct ttattaaata acaaaatatc cattgacttt gcaactccaa ctaaattaca
     6181 ataattataa ataaataatt attattggag gttttactat gtcattgatt gggaaaaaaa
     6241 tcgaagaatt taatgcacat gcttatcatc aaggagaatt tttagaagtc agtgaaaaag
     6301 atcttatggg gaactggagt attctttgct tctatccagc tgattttact tttgtttgtc
     6361 ctactgaatt agaagactta caagaacaat acagtacatt gaaatcttta ggtgttgaag
     6421 tcttttcttg ctctaccgat acccacttta ctcacaaagc atggcatgat acatcagatg
     6481 ctatcggtaa aatcgaatac gtgatgatcg gtgatccgtc tcatcagatt tctcgtattt
     6541 ttgatgtttt ggatgaagag caaggattag ctcagcgagg aacatttatt attgatccag
     6601 acggaatcgt tcaagctatg gaaatcaatg cagatggaat cggtcgtgac gctagtgcac
     6661 tgattgataa gattcgcgct gctcaatatg tacgtaccca cccaggtgaa gtttgtccgg
     6721 caaaatggaa agaaagtggc gaaacattga aaccaagttt tgatcttgtt ggaaagattt
     6781 aggtggtgga tatgttagac aaagaaacta aacaaaatct cgaacaatat ttagcactga
     6841 ttgaatctcc tattgttttt tctgttagtt tggataatag tgaaaatagt caaaaactag
     6901 ctgaatttac gaaggaaata gcagaaatgt ctcctaaaat ctcttgggaa aaaagagatt
     6961 cgatgcgcac acctagtttt tctatcaatc cagttggcaa agaaagtgga atcgtttttg
     7021 ctggtatccc cctcggtcat gaattttcct cttttttact ggctatgtta caaattagtg
     7081 gacgggcacc gaaaatcagt gaatctttga gtcttaaaat caaaaaaata acacaaacat
     7141 tgcattttga cagttatgtt agcttgactt gtcataattg ccccgacgtt gtacaggcat
     7201 tgaatatatt agctgtatta aatccaaata ttactcatac gatgattgaa ggtgggatgt
     7261 tccaaaaaga aatcgatgat aaaaaaatca tggcagtgcc aactgtgttt ttaaatggag
     7321 aagaatttag tagcggacgc atgacgatcg aacaaattgt tgaaaaaatc actggccctt
     7381 tgattgaaga agagttgttt gaaaaagaac cttatgatgt attagtaatt ggcggtggac
     7441 cagcagctag tagtgcagct atctatgcag cacgtaaagg aatccgtaca ggacttgtcg
     7501 ctgattcttt cggcggacaa gtcgttgaaa cactagggat tgaaaatatg attggtacct
     7561 cttacacaga aggtccaaaa ttgatgcagc aagtagaaag tcatgtgcgt agttacccta
     7621 tcgacattat gatgaatcaa caggccgttt ccttaaataa agagcaacat tttattaata
     7681 ttggcttagc taacggtata tcacttaaag caaaaaccgc catcatcgcg acaggtgcgc
     7741 actggcgttc tatcaatgta ccaggcgaaa aagagtttaa aaataaagga attgcttatt
     7801 gcccacactg tgacggccct ttatttaaag gaaaagagat tgctgttatc ggtggtggta
     7861 attctggtgt tgaagcagca atcgatttgg ctgggttagc aaaacatgta tatgttctag
     7921 aattcttacc agaattaaaa gcagatcaaa tcttacagga taaactagtc tctcttgcga
     7981 atgtcactgt tatcaaacat gcagcgacga aagaaatcag cggtacgaat caggtagaat
     8041 ctctctccta tatcgatcgc cgtacgatgg agatgcatac actagctgtt tcaggagtat
     8101 tcattctagt cggcttactc cctaatacgg attggctaga tggaacgatc gaaatgaatc
     8161 caagaaatga aatagtgacc gagaaaaatg gcgcaacgaa tatccccggc gttttcgcag
     8221 ctggtgattg tacagatagt ccatacaaac aaatcattgt ttcaatgggt tcaggagcta
     8281 ctgcagcttt aggagcattc gattatctga tgcgcacttg agctatgaag attgtaaaaa
     8341 gtcagagagc ggaaaaaagg atggtgacaa gaattttgtc acaatccttt ttgttttttt
     8401 gaataatcgc tttttcttct ctttttctta tatactataa gtagtaaata atttgaaaaa
     8461 aagaaaggtg actgattatg gcgcaaaatc gtatttttac gacaagtttt gcaagcgttt
     8521 atcctatgta tgtagcaaaa gcggagagaa aagatcgtat aaaagaggaa gtagatgaaa
     8581 tcatttgttg gctgacgggt tattcccaaa cagaactaga atatcaacta caaaaacaag
     8641 tgactttcga acaatttttt gcggaagccc ctgtccccaa tccttcacgt tccttgatta
     8701 caggtgttgt ttgtggtgta cgagtagaag acatcgagga taacttgatg aaagaaattc
     8761 gttatttaga taaaatgatc gatgagctag caaaaggcaa aaaaatggaa aagatcctca
     8821 gaagttgatt tttccagaga tattcttctc cttcgctcat tttgtaacat ttaatttggt
     8881 aaaatgacta gttcacgtta aaataagtca aaggaggttt tcttatatgt ttgagacgat
     8941 taaacaaaat attcaaaaat acgccttatt aagagctgct gtttatatta tttctgggat
     9001 tgcgattgtg ctaaaccctt ctgctgtttt tcattttatc ggttatctga tcaccgccta
     9061 tcttgttttg cttgggttgt tgaatctttt tgaggcttac cgttttaaaa ggaaaacagg
     9121 ttcttggagt atcggtctgt tcagtggtct ttttttcctg atcttagctc tgatcgtcta
     9181 tgcttttgca cctgcaatcg tttctatcct gccgattctt cttggattag ctgttatcat
     9241 caacagcttg ttccagttgt tcttcagcat gaatacaaaa gctaaaagtt ggagtgttta
     9301 tagtagtatt ctgttgttga ttggcggatt cgtcttattg ttcaatccat tcaaaagttt
     9361 gatgcttcta ttccaaatat tcggctttat tttgattttc atgggcatcc aggagattat
     9421 caatttcatc agaataagaa aaatgactat acgttaatca tttttagtta ttaaaataga
     9481 ccgtataaaa accaacaatc caaaaaaatc ttttggattg ttggtttttt tgtgagtata
     9541 ttttcctatt ttttcaataa tgtatgttat gctaagttcg ggctatattt tatacaaagg
     9601 aatgatgaga atggaattta ttggtatttt atgcctgata ttgatttcaa caacgattgg
     9661 ctcacatatc tcgcggagat taggtatccc agcagtgatt ggacaattac ttgttggtgt
     9721 tttgctaggc caagctggat tgaattgggt caaacctgat attctagtcc atgacttttc
     9781 ggagattggt gtgttcttat taatgttttt agctggtctg gaaagtgatt tatctctttt
     9841 gaaaaaatat ttccgacctg gtatgttcgt tgctttgctt gggatcatct tcccggttgt
     9901 ttttggctgg ttgacaggca aagcattcca agtttcggga aatgaagcga ttttctttgg
     9961 gattattctt gcggcaactt ctgtcagtat ttcagtagaa gtattaaaag aactaaacgt
    10021 agtgaataca aaagaaggat caacaatttt gggtgcttct gtggtagatg acattttagt
    10081 cgttttagtt ctaagtttca gcctatcttt tctaacgggg gaagcttcta gcaatgcttc
    10141 tttaccgctt gttttgattg aacagctaat ttattttgtt tttattttct tattagtcaa
    10201 atggattgcg cctttcctac tttcatttgc agaaaaagtg tacgcaaatt cttctattat
    10261 catcgtatcg ctagttattt gtttaggaat gtcttacgta gcggatcttg ttgggcttag
    10321 ctctgtcatt ggtgcttttt ttgctgggat agctgtcagt cagacaaagg ttaaagaaga
    10381 agtataccac aatgtagagg ctttaggata tgcagtattt ataccagtat tttttgtcag
    10441 tgtaggatta gaagttgatt tttctgactt tagtgaacaa ctactcttta tttctatatt
    10501 gacgcttgta gcgattttga ccaaattgct tggaggttat tttggtgctc agattgccgg
    10561 tttttctcaa catagttcat tgatggtcgg tgctggtatg atttcgcgtg gagaaatggc
    10621 tttgatcatt ttacaaattg gacagcaaag tcaattgatc gaaccacagt actattcacc
    10681 tttagttatc gtgattttat taagtacact tatttctcca cttgttctca aatattttac
    10741 aaaaaaagtt tatgaagtgt gagtaaatca aaaagaagtt gtgaaagaga catcgtgaaa
    10801 accgtttatt tgaaaaaagg actgtgacga taacgccaca gtccttttac tgtacttaaa
    10861 aagcaatttt ttattgataa ttttggttga ggtattgcaa tagttccgat gctcgtttgg
    10921 ttgctgaatc atcatcttct ttattttcca acatgctgac catcatataa ccttggttat
    10981 caagattgaa agcaaacaag aaactgttct cttgcccctt tacatcctgt ttttctttga
    11041 tttccgctgt accagttttc gctgccaacg gaatccctaa agcagaaaga gaatgtgctg
    11101 taccatttac atcttgcaca acttctctca gatctggcac gatcgtttgt actgctgttt
    11161 cgctgatgac attcttctta tcttttgtct ctttatctgc aatcaattta ggatagacaa
    11221 gtgtgccatt gttggtaaaa acagaataca ttgctgcttg ctggatagga gtaattagaa
    11281 gttcgccctg tccatatcca gtatcagcta gcaagatatc tgagttaaag ctatcttcat
    11341 tagaaatttg tgctggattc atactaattg gcaaatcaag gtcttcacca aaaatgaatt
    11401 tatctaagcc tgtacgaaat tttttctcac ccattttcaa cgtttcttgt gccgcatata
    11461 tattatcgga atagatcaaa gcagttttta agtctacttg tgatacatca ctactgacac
    11521 gcgttgtttt aaaagatccc caagaactgt ctttttgcca tttaagcccg ttgatcgtca
    11581 acacttcatt tggatcgata gtgccgttgt cgagaccgat tgctgctgta attattttaa
    11641 acgtagatcc aggagcataa cctgtcgcaa atcggctgat gaatggatgt tcaggatttt
    11701 cttcataagc tttgtaatct tcttgtgaga tcccgtttgt cattttgttt ggatcatagc
    11761 ttggagagct agcaagcgca agaagatcac cggtttttgg cgttgtcgca acagttgatc
    11821 cagcttttcc tcctagactg tcaaaagctg ttttttgtgc cttcgcatcg attgtcaatt
    11881 tgatatcttt gccattttga acttcatgtt cgatcagaac ttttttctct acaccgtctg
    11941 catctgtaat gcttaatttc acacctgttg tcccacgaag atccttatca aaagccatct
    12001 ccaaacctga gcgaccgatt tttccattac tgcttaattc tggattctta tcaatatctt
    12061 ctgctgtgat atctcccacg taaccaatca gctgagcagc tgcttcaccc aaaggataat
    12121 atctgccgtc tacttcttga atggtagctc cagctggaag ttctggcgtt gctccatcaa
    12181 tgattttcaa tgggacaaag taatcgggtt gtacccagct ttgtgaaatc gcctgattga
    12241 tagcatcttc tgttaagtcg aaggaggaag caatcgcttt gatattggct gttttttcgc
    12301 ctccatctcc aagtttactt ggaacgactc ctaattgttt tagtttgcct gttgttgcta
    12361 atggttcacc atttcgatct ataatattcc ctcttgctgc ttcttgtgtg gtcaggctta
    12421 ctttgtcatt tccttccatt tctggaaaaa ccaagttggg ttgccagttg atcgtggttt
    12481 gaccatcatt tctgtctaat gttcctttat aagaaagatc tttcaattca cctaatgagg
    12541 tattcatctt tgctttatat gaaaaactgt aagtagaatc gtctttttta tctactttta
    12601 gattcgatat ctgaagtcct ttgacatcag cagcaccgta tatattctgg tattgatcta
    12661 agatctcttt ttcagataat gcacttttat ttgccgcctt ttttgatgtc atttctgcag
    12721 ctttgttata atctcccttg ttcaaagctt ggacaaattg ctcaactgtc ttttctccag
    12781 cttctactgc ttgggtttct tgatagtgct ggtagtaaaa ataaccgcca cccgcagctg
    12841 ctattaagat cactgcgccg gcaatataag cgcctgttcg atttttgccg tgcttgtcac
    12901 ttcttttcat tttttcctcc ttctcttgct tgtcattcct ttatttcact atttatacct
    12961 gtttattcta acatatgtct ttgaggaaat ccataccaca atccagaagc acactttctg
    13021 gaaggttcgt ttggatacta ccaagtaatt tcccaaccgc tttgggtagc ttgaatattg
    13081 aagttacgtc tttcagctgc tgagagatag ctttgatcac ttgctgctgc cttctgatcc
    13141 tcccgaagtc tccttctttg tcttcccgaa atcgagcata ctggagtaaa gtattaccat
    13201 ccatcacttg ctctcctttg tttatatata cgccatctaa atctagagat ttttctgcat
    13261 cgatcttgac tccaccagga aacagttcat taacgcaatc gataaaatct tgaaatgtga
    13321 tacttgcata atagggagct tcaaatttga aattttcctt taatgtttga tttaataaat
    13381 caactccccc ataagagtaa gctgcattta tcttattcat tccgtaacct ggaatatcga
    13441 catatgaatc tctcatgata gagatcagtt ttgcttgttg tgttttctgg ttgtattgtg
    13501 caaccagaag tgaatccgag cgacctccac caatcgatct tttttcaccg catctcctcc
    13561 tcacacagca atccacttgt caggtgagga gcgagtttgt ctcatcaaat aagtcagtca
    13621 caaaaatata aagaaaaggt cagaaacctt ttcgaacaag aattctgacc tagcatcatc
    13681 tcatcgtccg agttttagca ctatacaacg taaagaaaac attcttagct attggaaaaa
    13741 gccttaatcc aacagtttct gttttaccca ttggcgactt tcgacgtttc tgggcaacag
    13801 cctatgtttg tataggagcc tcacctaaca aggttattac tatattctat ttacgagttc
    13861 atgatagact tttcctaatg attaggcaat ggaatttgaa atgaaatagt gaaatttaac
    13921 aatctttaat attttttaga aaaaaataac aaataagttg aaagaatccc gttgcaaatt
    13981 ctttcagttg caccaggatt ctttgataat tttttagttt tatgtgtttt tttatatgtt
    14041 ttttcacttt tcggaataaa tatttatttg ttaaagtaat taaacaatcc gctaatcgtt
    14101 tgatctggca tttctatttc cttaccatct aaagtaacag tagtaacgac aaaatggtct
    14161 tgcaatggcg gaacttctaa gtactgccct tgcagaaaat cattcactgt ttcatattct
    14221 tttttcattt cttttgaatc tgtcgatggt ttatagctta tttttaacat aactagtgct
    14281 cctttttatt cgggacgtgc aatcgtgaaa atttctccta ttgctgcata atcatttcct
    14341 gccaaacggc ttacagcatt caattttctt ggatcgatcc ttcctgtctt atcatcatag
    14401 actgtttcat ttaaatgata tttctgtact tctagcaaaa ataaatcagc tgttacttta
    14461 tttttattcc gtatctcgac atgttcatat aaggatgttt caaaacgaac ttttgcttct
    14521 gtcaatgctg gtactgaaat gattactgaa tcagttaaag tcaagtcagt caggtacagt
    14581 tcgctttcct cattaggcag attggctgct gttttattta cttcttctac gttgtcctca
    14641 tcaaggatat ggataacagc ttctcttgtc tcaagaatat tctttgctgt gtcttttact
    14701 gcatcttgtt ttctctggat cgccaaggat aagatgggtc gattcgaact gacgatattg
    14761 aagtaactga agggtgcgat attcactata tcttttgctg atttagtcgt tactactgct
    14821 actggtctag ggatgactga accaatcagc aacttgtaat tttcacgttc tgtcaattct
    14881 gaaggactcc gttcaaacat ggtttcgcct tctttctata aaattcgtta aaaagcagat
    14941 cttgaagaca aaatgaaaag taatgctttt ttcatcttct tagaatctgc tttaaactat
    15001 ctatcaattt ttcttttttg tcaagaatct gtgccgtgcg tgttttttag ttatttgctt
    15061 tacttgcggc ttcgacatat cttttagtga tcaattgcgg acgggtgata atagatccaa
    15121 ctacgactga aaaagcacct aattcatatg ctttctctac ttgttcagga gagtcagtat
    15181 gtccttccat aattactggg atatcgactt gttctatgat ttgattcaat aaatcaaaat
    15241 caggtgtctg cgtctttcgt gaatattctg tatagcctgc taaagtcgta gacaaaagat
    15301 caaaaccatc ttcttgcgct tgaagcgctt cctcaaaagt cgaaatatct gccatagcta
    15361 accgaccgtg ttcgtgaatc atttggacaa gttcagcagt tgttttttct gtcggacgtt
    15421 tacgagaagt tgcatccagt gcaatgattt ctgcttgcgt ctccaaaagt tcttccactt
    15481 ctttttctgt cgcagtaata aacacttctg acccatcgta atctcttttg ataatcccta
    15541 ttaccggtaa atcaacgatt cgcttgattt cattgatatc ttctgtcgta tttgctcgaa
    15601 taccaactgc tccgccttct tttgcagcca aagccattct ccccataata aaagaactgt
    15661 gtagcggctc gttttccaaa gcttgacaag aaacgacgac tccgcctttg atttgttcca
    15721 gtgtatccac aatatctcct ccagtaatgt tatataaaaa tacagcttca tataaaaatg
    15781 taaccctttt ctattcagga gacaataatg agctatttct ttcagcagac ctttcttact
    15841 aaaagaaaat tacttcaaat tcttcgtata cgaccagctt ctcttctgta aattctatgt
    15901 tcaacgcttc tttataagca tgtttaaaaa actcagcatg gactttccgc caataagcta
    15961 atgtccgatc cccttctcct tctttccaag catgctcttt cgtgacttca ctaaaaggca
    16021 agatggtcac atttatattt ttgatgatac aaactggttc attttttcca ttcaggacca
    16081 cgtctaattg accaactttt ggtaaaggct cattctcttg tttatacaga tcgtaaccac
    16141 ttgatgtacc agtctttatc ttttttacaa ctagttcgct caactcgtct gccatttttt
    16201 ctgtatcccc aaatgaaaag aaacctaatt tttctgattg ataactagga tatttttctt
    16261 ggaacttttt gacatattct tctacttgct gatacataac atccacccct ttgcttttta
    16321 taatgaaaaa accatccaga tactagtata aacaattatc tggatggtga gcataatgaa
    16381 ttattgaata ttcaagacac gcagactatc ttcaacgatt tgttttggtg ttaaatgata
    16441 acgttcataa agctgcccta caggaacgct atccgtgaat tctttctctg caccaaaatt
    16501 taatacgttc attgaagaat caccataata acgactgata ttttcgccaa atcctcctga
    16561 caaactacca tcttctaatg tgatgactaa ttcgtgtgtt tcttttaatt tatctaatgt
    16621 ttctacgtca ttttctgtga tcgttcctgg attgatcaac gttgcttcga ttcctttttt
    16681 tctaaaagct tttactgttt cttttcctaa ttcaagaaaa ccgcctaaag ctaaaattgc
    16741 tacttttcct ccattattta ctgtttgata ggctggaata ctataatcat ttgcgatagc
    16801 tgtaccatgt tctacaccat gtgtaggaat tcggattgct acaggctgcg ctgtttgttc
    16861 gaacgcccaa tctagcatag agatcatatc ctcttttgtt gctggagcaa gatatttgat
    16921 gttcgggata cttgataagt aaggaatgtc aaatgttcct tggtgtgttc ggtcactagc
    16981 tgagatcgtt ccgccaccga ctaaaataat cactggattc tcgttgatcg ctaaatcatg
    17041 tgataattga tcatatgccc gttgcaagaa agtacttgat tggaagacga ctggacgtac
    17101 tccctgagaa gccaaagcag aagcgaaagt gatagagtgc tgttcagcga tccccacgtc
    17161 aaaataatga tctggatgtt tttcttggaa tgttttcagt ccaaaggcgc ctggtatcgc
    17221 tgcattgact gcggcaatag gagctgtact tgtttgcatt ttcttatcca aataatccat
    17281 gatgatcata ttaaatgttt ctccagaagt tacttgcttt gcctccccgg tggacagatc
    17341 aaatggtaca tgccaatgat atttcatttt attatcgatt gccggctgat aaccgcgacc
    17401 tttttccgta tggacgtgaa gtacaatcgg atgatcaata tcttttactt cttcaaataa
    17461 acggatcacc gtttccagat cgtttccatt atccacgtaa cgataatcaa atcccattgc
    17521 tttaaatagg ttattgctgc ttttaccgtt ggtttgacga agctcagcta agtttttata
    17581 taaactgcca tgattctcat caatagacat ctcattatcg tttactaata cgattagatt
    17641 ggattgtaaa acaccaccat tgttcaatcc ttcaaaagct aagccaccac ttagggagcc
    17701 gtcaccgata aatgctacca catttcctga ttctttcttc agatctctcg ctttagctaa
    17761 acctgttgct aagctgatag atgtagatgt gtgccccact gtaaaatagt catgtttact
    17821 ttcatgttgg ttggaatatc cgctcacttc acggtaatgt cctttttcaa aaccattttt
    17881 tcgaccagtt aaaagcttat gaccataaga ctgatgggaa acatcccaaa cgattttatc
    17941 ctttggtgaa tcaaatacat aatgaaacgc aatcgttaat tctactacac ctagattcgg
    18001 accaacgtgc ccacctacat cgctgtcctt ttgcaagatt ttttggcgca tctcttctgc
    18061 caactgtttc atttcctcaa cagataattt tttgacatct gcggggctat tgattgcatc
    18121 caatacatgt aatactgtac tcataataca ttctctcctc cgtactatct tcttttaatg
    18181 aaaatagatt tgttacactt tatctattct actacttaga gttgactcta agtcaatagc
    18241 attttaaaaa aatttcagag aaagcttgtt atataagtag atcttcgcac gaatcaggac
    18301 tcaaataaaa accttctaca gacaattaaa aagctttgta gaaggtctcc taattaaggt
    18361 ctcatcaaga aatatgtttt tttaataggt gaagttcctg agcaatatga tacaaatgga
    18421 tgtttgcacc ttttaatgag attggataga tctttgtctt ttcagtttct aatccatttt
    18481 gatagctagc agcatcgcac aaaaccactc tttttttcgc acatagacat tcatcaggca
    18541 attcttctaa tgaacgaaat aaaataggat ttatttcagc gatgccttct tccgcataca
    18601 aatgaaattg ggttcgataa aaaaattgtt tgactgaact ttctgaaaca acaaaaattt
    18661 ctatttcctg tctccccgct tgttcactgt tgatcaaaga aagacttttg acgaattttc
    18721 gaatgaaatt ttcattccat cttagttgga tttgattttt ctttttccac tcttccaata
    18781 tttctacaat ttttccgtac agaagttgtt ctttttctga tagaagatag atcctttccg
    18841 gtaaaaatac ttgtccatct gcccacaacg tacggacaaa ttgtaaaaag gattgcttga
    18901 aaataaaatt ttctgcatcg tcgatttcag cgatactaat aatctgtaaa atcaattcat
    18961 tcagtgatag gtcattgaga acaaaatttt tataaacaac ttcgaaatct tttcttaaca
    19021 gctccatgtt ctcattggta taatttctac tgttgaacaa agaaaaaata taatagacat
    19081 cttcttcttg taagtctaga attccttttt gctcttccaa tccttgacag actaattgaa
    19141 ataacggctt cgtttctgcg cttttcttat cttcttcttt cagtccaagg ggaaatgcct
    19201 ttcttccgat tgcgatctct attcctcgta tgataaagga cttttcatct tccgaatatc
    19261 ttcttttaaa aaaatgagtc tctacataat caatcaactc gttacaagct tcttctacat
    19321 acggattttt attttcatag ccttcccaat tgacataacg tactaaagct agcaagatgt
    19381 tccgcgagtc aaattctggg ataatgattt ctttattttt caaataatca ttttctttaa
    19441 aaaaatccaa tacgattttt ttgattgtat atactttgct caaagaaata aattcaagat
    19501 cagccagttc tgtactcgtg aatataccat taaaataata acttaaaaac tttaaaaaat
    19561 aagattgacc gtacagaaac tgtgtgagct cagaaagagc aataccatgt ttgaatacga
    19621 gtttatactt atgcttctct cttacttgtt cttttataca atattctaga cactcggcta
    19681 gactttctag atcgtgaagg atcgttggtg tagtcaccct tagagaaata gctgtttgtt
    19741 cgatgtctat ttcttggaat tcaaataatt gttcacacag atacactttt ctaaatatat
    19801 ttcgctcgat gtaattttcc agcattcctg atacacgcct ccagtttttc ttatgatttt
    19861 atttaatcat atctgtaaat cgtatttttg tttccttttt atttagaaaa gccctttcaa
    19921 aaaccaataa aaaaagaaac tgggacaaaa gtcaaacaag ctcacaattt cagcctcatt
    19981 gtaggtagct ttgagccgtt tggactttta ccccagcctc tttcaagaat ggttattcaa
    20041 agatgtcagc gtctagttct ttgactacat ctttcaacaa ttggatacca gctgcgctgt
    20101 taccaaatgg atctagtgcc gggctgaaga cacctatgcc gtaacgattt ggcacagctg
    20161 ccatcaaccc gccgcctaca ccgctttttg caggtacacc aacatggacg gaaaactctc
    20221 ctgattcatc atacaaacca gcagttgtca taattgattt gacgattgta gcactttctt
    20281 cagtaatgat ttgtttgcca ttccaaggag caattccttt atttgccagc acagcagcta
    20341 attttgctat gcctgtagcg gtgacattga gggagcattg tctgaaataa gcatcgagta
    20401 tctccggtac gtctccttcg atcatttgat tacctttcat atagtaagca agagagcgat
    20461 tgatatcacc tgttcttgat tcagaatgat agatttcttc attcaatgtg atattaggat
    20521 catcacagat ctctttcata aatgacaaga ttcgctcaaa tttttcatct gcatctttac
    20581 cggcaatcaa cgaggttgtt gcaatcgctc ctgcattaac aaaaggattc attggatgtt
    20641 tgcgatgatt gatttccata ttcatgatag aattgaaggc aaaaccggtt ggttccgtgt
    20701 tgattgtttg aaagactttt tctattccgt tgtctttgat tgctaataac aatacgggaa
    20761 cttttgacat gctttcgatg gcaaagcgca catcggcgtc tcctccctca atcagttgtt
    20821 ttgtatccaa atcataaatc gccataccta attgtttggg atccacttca gctaatgctg
    20881 gaatgtaatc cgccacttgt cctgtttgat aatagtggaa attttttcca atcaattgtt
    20941 ctaattcttt tacttccatt tgaataacca gctttcattt tttatttttc tgcatgctgg
    21001 aatttatgac ggcatgcgta gatcgtaaat ggaataacta agacgataag ataagaaaca
    21061 atcagaatcg tttcataaat cccatgctgt ccagctgcga tactagcagg tggaacaaat
    21121 gagatacaaa aagcaaaaag tgaagtcaaa agacctaaac aagcaacgat tacttttaca
    21181 acttttccgc ctggaacctg ataggctctt tcaagactgt catctttaaa aaccagcttc
    21241 aaatagcccg cgaaaaacat aaagtacaac atcaggtaga ttacaacagt caatgacatc
    21301 gctgcaagaa atgaaagatt attccccccg ccgcttaatg taaagacggc tgcccagatt
    21361 gttgcaacga tcccatttgc gatcatcaaa tgagcaggaa caccgtgtgt gttttctttt
    21421 gcaaaatatt caggaagtaa tccttcttct gctgctacgt gaagtgcttt tgaaggagag
    21481 gttacccaag aagcaatttc tccgatgaca cctaaacaaa caagaatcgc aagaattttc
    21541 gcaatccatg caagatttgg atcaatgtgt cttaatagga attctaacga ttgaatcacc
    21601 cctgtattaa gagacaaacc atgttgaggt actgtagtag cgacggtcaa tccgccaaaa
    21661 gtatctaaaa tgattgccag aataaccaat acaaacattg ctaatggata atccttttta
    21721 gggttttcca tttcatttgc atgactggca gatgtttcta cgcccatata tgctaaaata
    21781 aaggaaacaa aaacaaccag cgtaggcaat ttcgaaaaat caggaatcaa tgttttcata
    21841 gacagtatcg tttgtagtgg agcgcctgaa gcgacataat ggatccctaa gccaaacaag
    21901 actaatgctg gcaacaagat accagctaca aaaccgatcc gagcaatcaa tgaagtgtat
    21961 ttggttcctt ttaactgtga gagtaaaata gcccagaaaa taatcaatag gattgccaat
    22021 ttcagatatt tattgttttg tatagcacca atacccgttg cataggataa tgcaccagtt
    22081 aagaaataca gcatggtcaa gtagccaact gttatctcaa accattggaa aaagattgcg
    22141 gcaaaaccga atcgttttcc caatgtgcga ctgacccaag tatagacacc gcctttttcc
    22201 catcctttca ccgttgccat ctctgctgcg cacaatgcca cagggataaa ccatagcaaa
    22261 ccgcctaata acaagaaaaa tactaagctg aagcctgagg tcgcgaatgt gggatattca
    22321 tataatgaaa cgaccattga gatggtcata gccaaaaagc caaagaacgt taattttttt
    22381 gcaggaattt tcgttttgct ttgttgcatg aatcttcctc ttttctattt gaatttgttt
    22441 caattgagtt tgttgccaaa gaagaaaaag tccaatcctt cttcttgttc ttcttttgta
    22501 gtagtgatcg ttgtaaagtg catcgccgat tttaaaatca ctgttgtcag cacataggca
    22561 tacacggcag taatgatgac accaatcact tgaatcccta agaagtacca gctgcttgga
    22621 ttcgttagta aatggctgtt agatgcaaag atccctatca gaatcgtacc tgtgaatccg
    22681 cccatgccat gaacacccca cacatcaagt gcatcatccc attgtttctt tttacgaaaa
    22741 tcaacagcag catgacagac gatacccgca atgatcccaa tcagcatggc actttttgct
    22801 tctacataac cggcacaagg cgtaatggct gccaatccag caacggatcc tgttagtacg
    22861 tcgacaaaat caaagccatt tccttttact tttgcaatga tcagccaagt aatcatcgca
    22921 aatgcaagag cgatgaatgt tgaaccaaat gcattgactg cttgttcatc tgctcgaagt
    22981 gcaccaccgg aattaaaacc gaaccagcca aaccaaagca gtcctgtacc gatagcagct
    23041 gccataagac tgctatgctg acttctttct cctaaaggaa tctttctttg accaagtgtg
    23101 aggatacatg ctaaagagcc gaatccagct gtcgtgtgga tgactgctcc accagcgaaa
    23161 tctctaaatc ccatctgatc caaaaatccg ccaccccata tccagtggca aacagggaaa
    23221 taaatcagca gattccaaaa aataacaagt ataatatagc cttttaaatt caatctccct
    23281 gcaaaagctc ctgtcatcaa aggaacggta atcacgataa acatcagttg gtaaagaaag
    23341 aacattaaaa atggaatcgt tgcaccatga aacatgttgg ggaagcttgt aacatgtctc
    23401 atcaaaaaga aatctgccgg gtttccaata acaccgccga tatcccgtcc aaaggccaat
    23461 ccgaatccac caaataccca cataatcgtt gttatcccaa cggagagaaa agattgagac
    23521 atgatcgtta gtatattatt cttccgtgct aatccgccgt aaaaaaaagc tagaccagga
    23581 gtcatcaggc aaaccattgc tgtacataaa atcatgaatg ctgtatctcc tgtattgata
    23641 ggtaatgtat tcattttttt cgcctccttt ctacgattac gagtataaaa gaaggtgtag
    23701 ttgttttttt cttctgtttt tttgaattaa tacttgcatt tataaaagac agacaattaa
    23761 taaaagcttg atgtgttgtg ctctttttcg tttttcacac aaataaagac aaaaaataaa
    23821 cggtcttctt tttatagaaa accgtttatt ttcgtctttt tttattttta aagatatttt
    23881 ttcataagga ataaagaata cttcatataa aatatataaa aaagaaccgc agcgagtgtc
    23941 gctacggttc tcaaatttgt tcttcatttc aacataagtt tttcttcaat ttgcttataa
    24001 tttacgtgca aagaatgaaa cgactaatac taaaatgatt gcaccgatca atgatggaat
    24061 gatagccatt cctgcaattg ttggtcccca agttcctagt aaagcttgtc caattgctga
    24121 acctactaaa ccagcaatga tgtttgcgat gatccccatt gatgatcctt tattcgtgat
    24181 tgctcctgca attgctccaa tgataccgcc gacgattaat gcccaaataa atcccatttt
    24241 aaattcctcc taactgactt cttacttctt attgtacacg tggttcgtta ttttctttga
    24301 ctttttgtgc acctttgcct acagcagatt ttgctttatc tgtttggcgt gaagcaaatt
    24361 gtcctgtgga ttttgctgct tctgttactt tgtcttgtac tgtttcttga tctttttggt
    24421 attcttcttg tgttttgatg tctgcaacat tcacattcac ttcgataaca tctaaatgtg
    24481 tcatattgtt tacttcagta ctgatcaatt ctttgatttg gttgtaaata tcttcgatgt
    24541 ctttaccgta ttctacgatc acatctagat ccacagctac ttgtttctta cctacttctg
    24601 tattgatacc tgctgttacg ttatctgtgt tgactaattt ttcagccata tttgagaaaa
    24661 atccgccatc taatgtcaat agaccatcta catctttcaa tgcataaccg atgatttttt
    24721 ggatgacttt gtcttcataa gtcaattcac cattcatcat gcggttttct cctttttcat
    24781 ttgccaaaac tgatccattt gttgtattca tgtttttgtt ttccatcttt gtttcctcct
    24841 atgatttaaa aaattgatca actattcctg actgtttcaa ataaaaaccg attcctgcgc
    24901 caattgctgt catgattatg atcaacaatg tcttgaaaaa tccaatcgtt aaccataaaa
    24961 cagctagcag tagtccaaga cccccaaaga taatagggag cttgtatgct gaaaatagtt
    25021 ctttcattat ttggccctcc tattccactc gagagtgctt gtagtctctt gtatcttctt
    25081 gttcttcata accgctgtat gtgactgcca ctttgaccgt ttcttttgtt ccgagtactt
    25141 tagttacttc ttcttcgata tcttgcatca atacacctgt tttccctact aatgaagaag
    25201 ttcgtttcaa gtctccgttg acatggatat ggattcgatt tttcgttgaa cgtatccgta
    25261 ctttaggtga atgaatgaac tcttcttcgt gtaaatggga acgtaccaac ccttcgatcg
    25321 cttttttgtc gattgttagt ttgccatctt cccgtttcat aacgaaagtt cctcttgact
    25381 ttggatagaa aagaacgact agcaagatga taatagcaag aacggcaaat actacagctg
    25441 cccagaaaat atattgttgc atgtagtaat tcgttaaagt aaagaagcga aatcgttcca
    25501 attgttcagg cataatatac ggcgcattga tcaacgcaat cgctagaaat actgaaagta
    25561 gcagtaaact catgataaca ccgattattt ttaacccctt attcattttc cacctcctat
    25621 aaacttattt ttaccctgta aataactcta ccgacaatca cagtatagat agcgaacagt
    25681 tagcatagca tcagtaaata aagcgctacc aaacattgct ggatcataac ggcgttttat
    25741 tgccagtctg atactgaaat aacaattgct tacttattct ctatttactt ttgattgctg
    25801 gctatactta aaggatatta ttcaagatta aaaattgcaa agattatgct cagttattat
    25861 tacaagattc tatttat
//
LOCUS       DACGLE010000035        25297 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0534
            SAMEA3304449-rid9560453.denovo.036, whole genome shotgun sequence.
ACCESSION   DACGLE010000035 DACGLE010000000
VERSION     DACGLE010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304449
            Sequence Read Archive: ERR985064
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25297)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25297)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702225.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702225.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 20:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,045
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 27 of 67
            Pseudo Genes (incomplete)         :: 25 of 67
            Pseudo Genes (internal stop)      :: 19 of 67
            Pseudo Genes (multiple problems)  :: 4 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25297
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304449-rid9560453.denovo.036"
                     /strain="VREN0534"
                     /db_xref="taxon:1352"
     gene            162..1103
                     /locus_tag="IXT20_001562"
     CDS             162..1103
                     /locus_tag="IXT20_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HAQ4860010.1"
                     /translation="MDYQVLLYYNYTKIEEPEEFAKNHLAFCKSLDLKGRILVAEEGI
                     NGTVSGTVENTTAYMKAMKADPRFADTMFKIDPSEGHAFKKMFVRYRPELVSLKLEED
                     IDPNQVTGAYLSPQEFKEAILDEQTVVIDARNDYEYDLGHFRGAVRPDIRSFRELPDW
                     IREHKEEFMEKRVVTYCTGGIRCEKFSGWLVREGFKDVGQLHGGIATYGKDPEVQGEL
                     WDGKMYVFDERIAVDINQVDKQIVGRDWFDGTPCERYINCSNPECNRQILASIENEEK
                     HLGACSIECAKHPRNRYVQDHGISPEEAELRFTALIK"
     gene            1172..1903
                     /locus_tag="IXT20_001563"
     CDS             1172..1903
                     /locus_tag="IXT20_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein phosphatase"
                     /protein_id="HAQ4860011.1"
                     /translation="MKSLIYAIGDVHGKHELFEKLIHYYDPKIHQLVLLGDLNDRGPR
                     TKECFLLGKQLVEETGAIYLRGNHEEYFLQFLQSPEDWYAPYMRNGGKETFESLIHPG
                     ASEEYSPTEISMMIQSRYSELIKFLVQRPLYYEWENYLFVHAGVDLTKKDWRKTSLRD
                     FIWIREPFHEGKNNTGKTIVFGHTITPMLHGDMQTTDLWIQDHKIGMDGGAVFGGSLH
                     GVVFDKEGILQDIEYQNNEGPWHGI"
     gene            2047..4227
                     /locus_tag="IXT20_001564"
     CDS             2047..4227
                     /locus_tag="IXT20_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HAQ4860012.1"
                     /translation="MTENLNQTIIQLVQKELSDYRPKQLTTVLNLLNEGNTVPFIARY
                     RKEMTGSLDEVQIREIEERYAYLENLEKRKTEVIRLIDEQGKLTPELEAEITQAVKMQ
                     QVEDLYRPYKQKRRTKATIAKEKGLEPLAKWLMQLTDGEVQSEAEKYIDKEKEVSSAE
                     EALHGAHEIIAEQVSDNAKFRTWIRSYTYNKGMYVSNVKDEQADEKGVYEMYYDFAEP
                     VHKMVSHRILATNRGEKEDILKVFFQVDEAAILAYLDRQIVKNPASPSSSFVREAYQD
                     SYKRFIQPAIERELRNELTEKADEQAIAIFGENLRNLLLQPPLKGKVVLGFDPAYRTG
                     CKLAVVDATGKVLAIEVIYPHKPAAQAKREAAGPAFIQLINKYQVDMVAIGNGTASRE
                     SELFVAEQLKSADHKTYYAIVNEAGASVYSASEIARKEFPHLQVEERSAVSIARRLQD
                     PLAELVKIDPKAVGVGQYQHDVSQKRLAEQLDFVVETAVNQVGVDVNTASPQLLQHIS
                     GLNKTTAQNIVIYREENGEFTARTQLKKVPRLGPKAYEQAIGFLRVPGGKNILDNTGI
                     HPESYSIAKEILTSVQLSEKELGTEEAVEKLTRLSVEKLAESLSVGEETLADILAGLT
                     QPGRDMRDEMPAPLLRTDVLSMEDLKPGMELTGTVRNVIDFGAFVDIGVKQDGLVHIS
                     KLSKKFVKHPTDVVSVGDIVTVWIEQVDTKKGRISLTMLSPYEE"
     gene            4247..4729
                     /locus_tag="IXT20_001565"
     CDS             4247..4729
                     /locus_tag="IXT20_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family protein"
                     /protein_id="HAQ4860013.1"
                     /translation="MTQEELQQLVEEISLKSFQLPFKHQAMFNPRLRTTGGRYHLNDH
                     HLDFNAKLFEESDEETIAGIIKHELCHYHLHLAGRGYQHKDMDFKYLLKKTGGLRYAP
                     ALTKKQIKMEYYRCQDCSATICRKRRIDVRKYRCGRCHGEIKWIETKREDFNEAQKIR
                     "
     gene            4745..5506
                     /locus_tag="IXT20_001566"
     CDS             4745..5506
                     /locus_tag="IXT20_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HAQ4860014.1"
                     /translation="MEKSEQRNLKDWNQLLNFLAAPEEGNFEELTAPTLILAGNCLPI
                     LADKAAQMYLNGQVDQLFLVGGVGHATRILYENFEKQGFHFEDGMSESEICRQYLKEV
                     YDLPDKAFLIESKSTNSGENATFSLEILHSLGAVPEKILLMNDPTLQRRTRATFEKVW
                     QNEQTIFANAVPFVPEILHFSEEIIFTAKELNHQWPKEYFHALVLGEMERLHDDENGY
                     GPKGKDFIPHIDISEEVWSSYTRIKESIKTDFSRT"
     gene            5637..6971
                     /locus_tag="IXT20_001567"
     CDS             5637..6971
                     /locus_tag="IXT20_001567"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HAQ4860015.1"
                     /translation="MENQNKLYHFVGIKGSGMSSLALVLHEQGLNVQGSDIEKYFFTQ
                     RDLEKANITILPFNADNVKPGMTIIAGNAFPDSHEEIQRAKELGLEVIRYHDFIGHFI
                     QNYTSIAVTGSHGKTSTTGLLSHVLSGVRPTSYLIGDGTGHGDPQAEFFSFEACEYRR
                     HFLAYSPDYAIMTNIDFDHPDYYTSIDDVFTAFQTMAGQVKKAIFAYGDDAYLRKLKA
                     NVPIYYYGVTENDDIQARNIERTTSGSAFDVYHGDEFVGHFTVPAFGKHNILNALGVI
                     AVAYFEKLDLKEVAEEMLTFPGVKRRFSEKIVADMTVVDDYAHHPAEIKATIDGARQK
                     YPDKEIIAVFQPHTFTRTIALMDEFAEALDLADKVYLCDIFGSAREEQGNVKIEDLGA
                     KIKKGGEVIKENNVSPLLDYHDAVVIFMGAGDVQKFEQAYEKLLSSTTKNVL"
     gene            7278..7763
                     /locus_tag="IXT20_001568"
     CDS             7278..7763
                     /locus_tag="IXT20_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydratase"
                     /protein_id="HAQ4860016.1"
                     /translation="MNIGKPRKLGKTINEIEEGDSLSLTESIEDNQLLLYLGLTNDAN
                     PLYIQHDFAQKTEYQQPIVPSVMLMGIITSAISKHLPGPGSHVVNFSVNFIEPVFHYE
                     TLTFQFEVIKVDKMKDVVTISVEAENTNEDRVLDAVVMVQPPQVPELEIAIESEGELN
                     E"
     gene            7756..8439
                     /locus_tag="IXT20_001569"
     CDS             7756..8439
                     /locus_tag="IXT20_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HAQ4860017.1"
                     /translation="MNNPSTSMSGINRFYAKVYGFLGMGLALSAVTSYLALWVFPLQL
                     ATFINNFPLGFVGLWIVELILVMVLGVKAQKNPSLAIGGFIAYSLLNGITLAVTLAYY
                     DIGTVTQAFLTATLMFMGMSVVGVTTKRDLSGVGRAGQAALIGVIIALVLNIFILRSS
                     VVELFISLLLVVIFAGITAYDNQSIRNIYVQTNGTAHSGIAVFMALKLYLDFINLFLA
                     FLRIFGRNN"
     gene            8565..11210
                     /gene="polA"
                     /locus_tag="IXT20_001570"
     CDS             8565..11210
                     /gene="polA"
                     /locus_tag="IXT20_001570"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HAQ4860018.1"
                     /translation="MTKNKLLLVDGNSVAFRAFFALHNSLERFKNRNGLHTNAIYAFN
                     TMFENVMQKEQPTHVLVAFDAGKTTFRTEMYAEYKGGRSKTPGEFKEQMPYIRDLLTG
                     LGVQYYELPNYEADDIIGTLAEKVDKDQFDVVVLSGDRDLTQLASKEVKVDITVKGVS
                     DIESYTPEHVAEKYDGLTPKQIIDMKGLAGDASDNIPGVTKIGEKTAIKLLKQYGSVE
                     GIYEHIDEMKQSKMKENLINDKEQAFLSKKLATINTSAPVDVSIDSLKYEGKNLDKLV
                     PFYKEMDFNQFLSKLNIVEEEVKMDDILFEVVHEFKEEMFTTDMALYVEMMGDNYHTE
                     EIVGVAWGTEKKIYVTNDLSVFENRAFHSWITDPTRLKKVYDAKRTYVALNRYTGKTK
                     GIDFDVLLGAYLLDTNEKSTDIEGIAAHYGYNDIQSDEAVYGKGAKKGLPEEEELFFA
                     HLARKVAAINALTPKLSQELADKNQEDLFRKMELPLSIILAEMEISGIKVDATRLQEM
                     KGEFSARLREIEQKIYEEAGEEFNLNSPKQLGVILFEKMGLPVIKKTKTGYSTAVDVL
                     EQLREQAPIVEDILTYRQIAKIQSTYVEGLLKVIQSDGKVHTRYVQTLTQTGRLSSVD
                     PNLQNIPIRLDEGRKIRQAFVPREKDWLIYSSDYSQIELRVLAHISNDEHLKAAFLEG
                     QDIHASTAMRVFGIEKAEDVTPNMRRQAKAVNFGIVYGISDYGLSQNLGISRKAAQQY
                     IDTYFEKYPGVKEYMERIVREAKDQGYVETLYHRRRYLPDINSRNYNIRSFAERTAIN
                     TPIQGSAADILKIAMIELDKRLKETGLQATMLLQVHDELVFEVPEKELESLDKLVKEV
                     MEQAVSLHVPLITDSSWGKTWYEAK"
     gene            11255..12091
                     /gene="mutM"
                     /locus_tag="IXT20_001571"
     CDS             11255..12091
                     /gene="mutM"
                     /locus_tag="IXT20_001571"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HAQ4860019.1"
                     /translation="MPELPEVETVRKGLERLVVGKTIQKVQVLWPKIIEQPETPIFEA
                     SLVGETIQSIGRRGKFLIFHLDHCELISHLRMEGKYQFTKENTPIDKHTHVLFFFEDG
                     SQLRYNDVRKFGRMTIVEKGASATYRGIMKLGPEPLPDSFLLADFANGLKKSHKAIKP
                     LLLDQRLVTGLGNIYVDEALWEAKIHPEQPANTLRPKEVEQLRLSIIDVLDRAIEAGG
                     TTIRSYLNALGESGGFQVALHVYQQTGKPCIRCGTPIVKTKVAQRGTHYCPNCQRLKI
                     AK"
     gene            12055..12684
                     /locus_tag="IXT20_001572"
     CDS             12055..12684
                     /locus_tag="IXT20_001572"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HAQ4860020.1"
                     /translation="MSELSKAKNRKVGFVLGLTGGIATGKSTAAKVFQSHGFPLIDGD
                     VIARETVEPGTLALRKIVSVFGQEVLQPDGQLDRGKLGMIVFPSKEKRQKLDQLLDPF
                     IRKAIKDQIASLSSNHPLVIVDIPLLYEGHYDHYMDAVAVVYTTPETQLQRLMKRNQL
                     TLEQAQQRISSQLPIEEKKQRADILFDNNGTKEKLVGQIENWLFANRFL"
     gene            12736..13068
                     /locus_tag="IXT20_001573"
     CDS             12736..13068
                     /locus_tag="IXT20_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HAQ4860021.1"
                     /translation="MGSLKNQKDKVVGNAKEKIGEMLNKENLIDEGRTQSETAEEKEK
                     QYQARIDEIEDQQAEEEAKRQDLTQERKNPTPDRSKTPLPESDHISDEQKIEEQRMKH
                     YTGIEEKL"
     gene            13380..13871
                     /gene="nrdR"
                     /locus_tag="IXT20_001574"
     CDS             13380..13871
                     /gene="nrdR"
                     /locus_tag="IXT20_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor NrdR"
                     /protein_id="HAQ4860022.1"
                     /translation="MRCPRCQHNNSRVIDSRQADDGRAIRRRRECENCSFRFTTFERI
                     EAAPLLVIKKNGEREEFNRDKILRGLIRSAEKRPVAMEQMEQIVNHVENRVRSLGENE
                     VSTNLIGEYVMEDLVDLDEIAYIRFASVYRQFKDMSVFLKELQDIVDKAKENNKENNK
                     ENN"
     gene            13894..15267
                     /locus_tag="IXT20_001575"
     CDS             13894..15267
                     /locus_tag="IXT20_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Replication initiation and membrane attachment"
                     /protein_id="HAQ4860023.1"
                     /translation="MENAWKELQPKNIYQGRKAAPLREDSKNALLYLYQPLIGGEALS
                     IYFTLLSEISQESGKGPEGMHADLLSSLGCGIPQFYDARKKLEGIGLLDVYFKEDSDL
                     GPRFIYELLEPLIPKAFFKDSVLAFLLLEKVGERRFQKLTQFFEPKRHTAEGYQKITK
                     KFLDVYRFNEAVYAANQENIESLNKSFEESESVALLEADSTFDWQFLNDWLAKKHIAS
                     LDDPIIKQVDMYHQLYGFDELTLGELISQSYDFSEQKVSEKELQKAILAMQRRPVKQT
                     KETVAVSENSNEPSSNEHLTNAALQLIKEAQSIPPMKYLEAIKSEKGGYVSKQENWLL
                     QDLVSQSGLSSSVINVLLNYVLVIKNNASLNASYVNTVANEWAQKKIATAEEAIQHIR
                     QISNQAKQSKQKKQTNYQTGKRNVRREKLPDWVNQPKDEKQISSEKKAEIDRRFKEYL
                     AKKEGDI"
     gene            15267..16193
                     /gene="dnaI"
                     /locus_tag="IXT20_001576"
     CDS             15267..16193
                     /gene="dnaI"
                     /locus_tag="IXT20_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaI"
                     /protein_id="HAQ4860024.1"
                     /translation="MEDVGKELKRIISKRNYQEKYAEMIAEVLNDADVQAFLSAHQEE
                     LTQADIEKSYAKLYEFVQEKRKYQKNDPTMIAPGYEPKLTLNFHYVDVTYVPTEALLA
                     RQRQEEIRSRVKAMDMPKDIQSASFENFERTDGRGYASLEALDFVEKYTADPKGYHKG
                     LYLVGSFGIGKTYLLGATARELAIAGFTTTLVHFPTFAVEMKQAIGKDQVAEKLDAVK
                     RSPILMIDDIGADAMSSWIRDEVFGVILQYRMQEQLPTFFSSNFTMDELEQHLSVTQR
                     GDEEPLKAKRIMERIRYLTKEIEMTGRNRRNG"
     gene            16322..16762
                     /locus_tag="IXT20_001577"
     CDS             16322..16762
                     /locus_tag="IXT20_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HAQ4860025.1"
                     /translation="MRAIFSSISVVGLFFLLAGCGSADSNTGSVDSIDAGQTVKTSST
                     KTERQSIFVGTIESITQVEAEEETLQLKLVDVMAKEDPANMGTSFSNDGVILNATAEK
                     ISVDINELKIGDKVQFTLVEMPIMTMSIPPQVPGDSIIDVSLNK"
     gene            16906..17607
                     /locus_tag="IXT20_001578"
     CDS             16906..17607
                     /locus_tag="IXT20_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine-N(1))-methyltransferase"
                     /protein_id="HAQ4860026.1"
                     /translation="MNHLDLSQRLAAVGDFVPESARLADIGSDHAYLPVALILRGKIE
                     YAVAGEVVQGPYESARKQVKKDGLEDRITVRLANGLDAIQPQDGITAVTIAGMGGSLI
                     RDILEAGKLNQRLSGQERLILQPNIGERTLRSWLQENQYKIQSEKILEENKKIYEIIV
                     AEKEQKPVTYNEMELMFGPFLLEEKSEIFKSKWTRELQQRKAIREQLKQAANQEARQK
                     QIEQEIQMIEEVLKP"
     gene            17604..18725
                     /locus_tag="IXT20_001579"
     CDS             17604..18725
                     /locus_tag="IXT20_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HAQ4860027.1"
                     /translation="MTISGRTFIQKFESYCPQWLAEEGDPVGLHIGTLDKPIQRVMMT
                     LDVRPEVVEEAIKKKIDLLIAKHPPIFRPVKRLVTDQPQEKMYADLLKHDIAVYAAHT
                     NMDIIWDGLNDWFCELLGIEVESYLVKTHEIHYKKLAVYVPVDHAQKMREVLAAAGAG
                     TQGDYTGTSFTSIGHGRFTPEAGAQPAIGKVGKTEQVQEAKVEVILPETIEKQVIQAM
                     RSAHPYEEPAYDLFAIDEPVEMFGLGRVGELPQEISIEAFVEQVKEAFQLDGLRIVQP
                     KNAKSSVKRIAICGGSGEKFYPQAIAQRADVYITGDIYYHTAHDMQSAGLIAIDPGHY
                     IESLCKQRFIEKFESWKQEENWDINFFVSETNTNPFQFN"
     gene            18740..19972
                     /gene="pepT"
                     /locus_tag="IXT20_001580"
     CDS             18740..19972
                     /gene="pepT"
                     /locus_tag="IXT20_001580"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HAQ4860028.1"
                     /translation="MYENLLPRFLRYVKTETRSDATSKTTPSTQTQVAFAQELKKELE
                     ELGLADVFYNEDNGFVMGTLPSNVDHEVRSIGFISHMDTADFNAVGVNPQIIENYDGE
                     STIVLDAEERYTLNTKDFPNLKNYEGHTLITTDGSTLLGADDKSGIAEIMTAMEILIN
                     NPSIPHGTIKVAFGPDEEIGVGADKFDVEQFDVDFAYTVDGGPLGELQYETFSAAQAN
                     ITIQGKNVHPGTAKDTMINALQLAIDFHNQLPADEVPEKTEGYEGFFHLMALDGNPEE
                     ASMSYIIRDHDREKFEARKAQITHIQEELNQRFDQERIKVDMYDQYYNMKEIIEKDMS
                     IVELAKQAMIELGISPVIEPVRGGTDGSKISYMGIPTPNLFAGGENMHGRFEFVSLQV
                     MEKATDVIVKIAELNTKS"
     gene            20104..21012
                     /locus_tag="IXT20_001581"
     CDS             20104..21012
                     /locus_tag="IXT20_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegS/Rv2252/BmrU family lipid kinase"
                     /protein_id="HAQ4860029.1"
                     /translation="MEKLLVFYNKSSGKDEGEKLARWFQDYVKEKQPELYVELAETGP
                     SIDDAELVKKAEAIEADTLVIIGGDGTIHHIVQAFQKTLSQYHVGLLPGGTVNNLARV
                     LEIPLKKEEAADVILEGNVRKIDYGQVNDEVIISTLTIGILADTAVWISQEEKQRYGS
                     WIFIKKFFKLLLKKKKYHLDLETDEEHWKGKSQLLTVTMSNSVGGFTNFDDSATLDDG
                     KFHVTIVPSLDIFRFIFYLPRIMRGKFYTIPGITYFTAKRMKIQAQEKSVGTRTDGDT
                     TDDLPVELSVVSKGLTVYSPKKEEKA"
     gene            21047..21319
                     /locus_tag="IXT20_001582"
     CDS             21047..21319
                     /locus_tag="IXT20_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HAQ4860030.1"
                     /translation="MDVLLHNLLWFYLFIVNFYLFCLMGYDKHQARTSGWRVPENNLI
                     FVSLLGGGLGGLISRKVFHHKTRKKKFTIGFAAGVLVDLILIYFFH"
     gene            21330..22376
                     /locus_tag="IXT20_001583"
     CDS             21330..22376
                     /locus_tag="IXT20_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase-like domain-containing protein"
                     /protein_id="HAQ4860031.1"
                     /translation="MSSKGTKTKIILNLLLLIVIFAIIYYLINQSFADIFAELLSTSV
                     VVLAAAVILGTVYQIAEGRSIKEIAEPFAPNFTTKDGFLTSSYIAFYRIVSFGTGTLI
                     SEIYFYNKKGMKLSQGAGVTALHMIMYKLAVITYALAGLVIQFSLFYSNGPSMIWFII
                     AGILLTIVIVAFLLAMSVSLNVQIFFVSLSNKLFKSERLRKWVDTCNQQIYSLRETVQ
                     TIIQDRTALLRIYLWNMLKLVFWYVLPYLILIENHPKIDFLLTISFISFAVVLSGVIP
                     TPAGIGSFEFVYLLLFRPVVGTVDAVSSLLLYRFSSFILPFLYGFVYVIAERRRVIKE
                     EIIEVKKENKENQG"
     gene            22627..25236
                     /gene="clpB"
                     /locus_tag="IXT20_001584"
     CDS             22627..25236
                     /gene="clpB"
                     /locus_tag="IXT20_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HAQ4860032.1"
                     /translation="MNIEKMTTTLQEAIAEAQQIAVTRKHQDIDIAHVWKIFLQPNHF
                     GRNFYTDAGLDVESFEHEIDRLLDEYPVVSGGNVQYGQNLSQNFFRLLNEADQIRESF
                     GDEFLSTEVVILALMKLKNYPLTVYLNKNGLSEKELRKNIEEMRGGERVTSQNQEEQY
                     KALEKYGVDLVQQVKSGKMDPIIGRDEEIRDVIRILSRKTKNNPVLIGEPGVGKTAIV
                     EGLAQRIVRKDVPENLKDKTIFSLDMGALIAGAKFRGEFEERLKAVLKEVKKSDGRIL
                     LFIDEIHNIVGAGKTEGSMDAGNLLKPMLARGELHLIGATTLDEYRQYMEKDKALERR
                     FQKVLVKEPTVEDTISILRGLKERFEIHHGVNIHDNALVAAATLSDRYITDRFLPDKA
                     IDLIDEASATIRVEMNSMPTELDQVTRRLMQLEIEEAALKKESDDASKKRLKNLQEEL
                     AELREEANAMKMQWETEKEEVNSVSAKRAEIDKAKHELEDAENNYDLERAAVLRHGTI
                     PQLEKELKELEAKAKDSEIKMVQESVTENEIAQVVGRLTGIPVTKLVEGEREKLIKLN
                     ETLHKRVIGQDEAVDAVSDAVIRSRAGLQDPNRPLGSFLFLGPTGVGKTELAKALAEN
                     LFDSEDHMVRIDMSEYMEKHAVSRLVGAPPGYVGYEEGGQLTEAVRRNPYTIVLLDEI
                     EKAHPDVFNILLQVLDDGRLTDSKGRVVDFKNTVLIMTSNIGSQLLLEGVTADGTIPE
                     AVAEQVNTLLRGNFKPEFLNRIDDTILFTPLSLDNVKGIVDKMVAQLAQRLEHQEILL
                     TISDEAKTWIAENAYEPAYGARPLKRFITKEVETPLAKEIVAGHVMPKSKVTITLLDG
                     QLHFKTEELEEIV"
BASE COUNT         8578 a         4407 c         5553 g         6759 t
ORIGIN      
        1 ttttctgtgc gaaacaggaa agcgaagtct ttttaattca aataataata ttatgtaaac
       61 aaaaaacttt ccatgcgtaa ttatacacaa gcaaataaat cgtcaaatgc gaacgccttc
      121 tttatactgt ttaggaacga aaagaaggaa ggaagaaata catggattat caagtactgc
      181 tttattacaa ttatacaaaa atcgaagaac cagaggaatt tgccaaaaat catttagcct
      241 tttgcaaatc tttggatctt aaaggtcgta ttctagtagc tgaagaagga ataaatggta
      301 ctgtgtctgg aaccgttgaa aacacgactg cctatatgaa agcaatgaaa gctgatcctc
      361 gatttgctga tacgatgttc aagatcgatc caagcgaagg acatgccttt aagaagatgt
      421 ttgtaagata ccgtccagag cttgtaagtt taaagctaga agaggacatt gatccaaacc
      481 aagtgacagg tgcctatctt tcaccgcaag aatttaaaga agctatttta gatgaacaga
      541 cagtggtgat tgatgcacgg aatgattatg aatatgactt gggacatttt cgaggggctg
      601 tacgcccaga tatacgcagt tttcgagagc ttccagactg gatccgcgag cacaaagaag
      661 aatttatgga aaaacgggta gtgacctact gtactggtgg cattcgttgc gagaagtttt
      721 ctggttggct tgtccgtgaa ggattcaaag atgtcggaca gctgcatggc ggtattgcta
      781 cttatggtaa ggatcctgaa gtccaaggag aattatggga cggcaagatg tatgtgtttg
      841 atgaacggat cgctgtcgat atcaaccaag tagataaaca aatcgtcggt cgcgactggt
      901 ttgacggaac accatgtgaa cgatatatca attgtagcaa tcctgagtgc aatcgtcaga
      961 ttcttgcttc cattgaaaat gaagaaaaac atttaggagc atgtagcata gagtgtgcga
     1021 aacatccgcg caatcgctat gttcaagacc atggaatctc tccagaagaa gcagaactgc
     1081 ggtttacagc tttaattaaa tagaaaacaa tagttttgat tgtcgaccat ttccaatatg
     1141 cgctatacta gcgttgagga tggtgatcca catgaaatca ttgatttatg ctattgggga
     1201 tgtccatggc aaacatgagc tttttgaaaa acttattcac tattatgatc caaagatcca
     1261 tcagcttgtt ttattagggg atctcaatga tcgaggtcct cgtacaaaag aatgtttttt
     1321 attaggaaag caattagtcg aagaaacagg agccatttac ctccggggaa accatgaaga
     1381 atattttttg caatttctgc aatcaccgga ggactggtat gcgccttata tgagaaatgg
     1441 tggaaaagaa acgtttgaaa gcctgattca cccaggggca tcggaggaat actccccaac
     1501 tgaaatctcg atgatgatcc aatcgagata ttcagaactg attaaatttt tagtccagcg
     1561 ccccttgtat tatgaatggg aaaattatct ttttgtccat gccggtgtgg atttgacaaa
     1621 gaaagactgg cgtaagacaa gtctaagaga ctttatttgg ataagggaac catttcatga
     1681 agggaaaaac aatacaggta agacaattgt atttggacat acgattacac caatgctcca
     1741 tggagatatg cagacaacag acctctggat tcaggatcat aaaatcggaa tggacggtgg
     1801 ggctgtattc ggagggtcat tgcatggggt tgtttttgac aaggaaggta ttcttcaaga
     1861 tattgaatat cagaacaacg aaggaccttg gcatggaatc tgactaatgg aaagaatgaa
     1921 ggagctgtga ctttgttgcg gctcttttta tttttataga gaatattgtt tctttcagta
     1981 gtttaaccca ggataaaaag tatgatagaa taaagaagca atttttagca gaaaagaggc
     2041 attttaatga cggaaaattt gaaccagacg atcattcagc ttgtacaaaa agaactttca
     2101 gactatcgtc ctaagcagtt gactactgta cttaatttgt taaatgaagg aaacactgtt
     2161 ccttttatcg cgcgttaccg aaaagaaatg actggaagtc ttgatgaagt ccagattcgt
     2221 gaaatcgaag agcggtatgc ctatcttgaa aacttagaaa aacgtaaaac tgaagtgatt
     2281 cggctaatcg atgaacaagg gaaactgact cccgagttag aagcagaaat cacccaagcc
     2341 gtgaaaatgc aacaagtgga agatttgtat cgtccataca aacaaaaacg acggacaaaa
     2401 gcaacgattg ctaaagaaaa aggacttgaa ccactcgcga agtggctgat gcagcttaca
     2461 gatggagagg tgcaatctga agcagaaaaa tatattgaca aagaaaaaga agtctcttct
     2521 gccgaagaag cgttgcacgg tgctcatgag attattgctg aacaagtcag cgataatgca
     2581 aaatttcgga cttggattcg gtcttatacg tataacaaag gtatgtatgt cagcaatgtc
     2641 aaggatgagc aagcagatga aaaaggcgta tatgaaatgt attatgactt tgcagaaccc
     2701 gtccataaaa tggtttctca tcgaatatta gctactaatc gtggagaaaa agaagatatt
     2761 ctgaaagtat tctttcaagt agatgaagca gcaattttag cttacttgga tcgccaaata
     2821 gtcaaaaatc ctgcctctcc ttcttcttca ttcgtcagag aagcctatca ggatagttat
     2881 aaacgcttta ttcagccagc aatcgagcga gaactgcgca atgaattgac tgaaaaggca
     2941 gatgagcaag caatcgctat tttcggcgaa aacctccgta atcttttgtt gcaaccgcca
     3001 ctaaaaggta aagttgtcct gggatttgat ccggcttatc gtactggatg taaactagca
     3061 gtcgtagatg cgacagggaa agttttagca atcgaagtta tttatcctca taaacctgca
     3121 gcgcaagcaa aaagagaagc tgctggacca gcttttatcc aattaattaa taaatatcaa
     3181 gtagatatgg tagctatcgg aaatggtaca gccagtcggg aatctgaact ttttgtggct
     3241 gagcagttga aatccgccga ccataaaacc tattacgcta tcgtcaacga agcaggcgct
     3301 tctgtttact ccgctagcga aattgcacga aaagaatttc cgcatctaca agttgaagaa
     3361 agaagtgctg tttctattgc ccgtcgcctg caagatcctt tagctgaatt agtcaaaatc
     3421 gatcctaaag ctgtcggagt aggccagtac caacatgacg tttctcaaaa acgtttggct
     3481 gaacaattgg attttgttgt ggaaacagcg gttaaccaag taggtgtgga tgtgaataca
     3541 gcaagcccgc agctcttgca gcatatatca ggattgaata aaacaactgc ccaaaacatt
     3601 gtcatttatc gtgaagaaaa cggagaattc accgcacgaa ctcaattgaa aaaagtgcca
     3661 cgcttaggac caaaagcata tgaacaagca atcggattct tgcgagtccc tggtgggaaa
     3721 aacattttgg acaacacagg tatccatccc gaaagttatt ctattgcaaa agaaatatta
     3781 acttcagtcc aactttcaga aaaagagctt ggtacagaag aagcagtaga aaaattgaca
     3841 cgattatccg tagagaaact ggctgaaagc ctatcagtcg gagaagaaac gttagccgat
     3901 attttagctg ggctgacaca acctggacga gatatgcgtg atgagatgcc agcgccctta
     3961 ttacgtacag atgtattgag tatggaagac ttgaaaccag ggatggaatt gactggaacg
     4021 gtccgtaatg tcattgactt cggcgcattt gttgatatcg gtgtgaagca agacggactt
     4081 gtccatatat cgaaactcag caaaaaattc gtcaagcatc ctacagatgt cgtgtcagta
     4141 ggagatatcg taacagtttg gatagaacaa gtcgatacga aaaaaggcag aatcagtttg
     4201 acgatgcttt ccccttatga agagtaacaa tgaggagtaa caaaaattga cacaagaaga
     4261 actgcagcag ttagtagaag agatatcatt aaaaagcttc cagcttcctt tcaagcacca
     4321 agcaatgttc aatccacgct tacgtacaac tggcgggcgc tatcatttaa acgatcatca
     4381 tttagatttt aatgcgaagc tatttgaaga gtcagacgaa gagacgattg caggaatcat
     4441 caaacatgaa ctttgccatt atcatcttca tctagctgga agagggtatc agcataaaga
     4501 tatggatttc aagtatcttt tgaaaaaaac aggagggttg cgctatgcac cagccttgac
     4561 gaagaaacag ataaagatgg agtattatcg atgccaagat tgttctgcca cgatctgccg
     4621 caagagaaga atcgatgtac gcaaatatcg ttgtggaaga tgtcatggag aaatcaaatg
     4681 gattgagaca aaaagagaag actttaatga agcacaaaaa atcagatgaa aggatagaag
     4741 gcctttggaa aaaagtgagc agaggaatct aaaagactgg aatcagctat taaatttttt
     4801 agccgcacct gaagagggaa attttgagga actgacggca cctacactta ttttagctgg
     4861 aaactgtctt cctatcttag ctgataaagc agcacaaatg tatttaaatg gccaagtaga
     4921 tcaattgttt ttggtcggag gggtggggca tgcgactaga atcctttatg agaattttga
     4981 aaagcaagga tttcattttg aagatggcat gagtgaaagt gaaatctgtc gtcagtatct
     5041 aaaagaagtc tatgatttgc ccgataaggc ttttctgata gaatcgaagt ctacaaattc
     5101 tggagagaac gccacttttt cactggagat tcttcattcc ttaggagctg taccagaaaa
     5161 aatcttattg atgaatgatc cgacactgca acgcaggact cgagcaactt ttgagaaagt
     5221 atggcaaaat gaacaaacta ttttcgcgaa tgctgtgcca tttgtgccgg aaatccttca
     5281 cttttctgaa gagatcatct ttacagcaaa agaactgaat catcaatggc ctaaggaata
     5341 tttccatgcg ctagtattag gagaaatgga acgtctccat gacgatgaga acggctatgg
     5401 tccgaaagga aaagatttta ttcctcacat tgatatatca gaggaagttt ggtcttctta
     5461 caccagaatt aaagagtcaa taaaaacgga tttttctcgt acttgaccat tttagcaagc
     5521 gaatcctcct ttccaaataa atttggttga aattttcaat cgtttataag ataatgagga
     5581 gcgtgttgaa tgtaatagct aaaagaatgg acgctagata agagaagagg attcaaatgg
     5641 aaaatcaaaa taaattgtat cattttgttg gtatcaaagg ttcaggaatg agctccttgg
     5701 ctcttgtttt acatgaacaa ggtctaaatg tccaaggatc agacattgaa aaatatttct
     5761 ttacacaaag agatttagaa aaagcaaata tcacgattct gccatttaac gcagataatg
     5821 taaaaccagg gatgacgatc attgcaggaa atgcatttcc tgattcacat gaagaaatcc
     5881 agcgagcaaa agaattaggt ttggaagtga ttcgctatca cgactttatt ggtcatttta
     5941 tccaaaatta tacgagtatc gctgtaacag gctctcacgg aaaaacaagt acgactggac
     6001 tgctctctca tgtattgagt ggtgtccgtc caacaagtta tctaattgga gatggaacag
     6061 gacatggtga tccgcaagcg gaattctttt catttgaggc ctgtgaatat cgccgtcatt
     6121 tccttgccta ttcaccagac tatgcaatca tgaccaatat cgattttgat catccggatt
     6181 attatacaag tatcgacgat gtatttacag ctttccaaac aatggctgga caagtgaaaa
     6241 aagctatttt tgcgtatggt gacgatgctt atttacgcaa attaaaagca aatgtgccga
     6301 tttattatta tggtgtcaca gaaaacgatg atatccaagc acgaaacatc gaacgcacaa
     6361 cttctggttc tgcttttgat gtctatcatg gggatgaatt tgttgggcac ttcactgtac
     6421 cagcattcgg taaacacaat atcttaaatg ctttaggtgt tattgcagta gcttatttcg
     6481 aaaaactgga tctgaaagaa gttgcagaag aaatgctgac tttcccagga gtaaaacgtc
     6541 gattcagtga aaagatcgta gcagacatga ccgtagtcga tgattatgcg catcaccctg
     6601 cagagatcaa agcaacgatt gatggcgcac gccaaaaata tccagataaa gaaataattg
     6661 cagttttcca accccatacg tttacgcgta cgattgcatt gatggatgaa tttgcagaag
     6721 cacttgattt agctgataaa gtttacttat gcgatatttt tggttcggct cgggaagaac
     6781 aaggaaacgt taagattgaa gaccttggag cgaaaatcaa aaaaggtgga gaagtcatca
     6841 aagaaaataa tgtatctcca ttgttagatt atcatgatgc agtagtaatc ttcatgggtg
     6901 ctggagatgt tcaaaaattt gaacaagcgt atgaaaaact tttaagcagc acgacgaaaa
     6961 atgtactgta atacaattac tgcagtgtcc ttataaaaat agaaagaggg ggagtggcaa
     7021 aaatcttgcc aagccccctt ttttgcaact tatgataaac ggtgttcaaa agcttgcatc
     7081 tgcggtaata agcccaatca aatcaaaaac atgagaagct gttttttctt ggctgttctt
     7141 attatttgat gcaggacagc tttttcacga cctctgtaaa ctataggaat ctgttaaatc
     7201 aagggagcag aacttgcctt ttacgctact tttggtacaa taatgtatga ttaaaaataa
     7261 actggagggt ttctatcttg aacataggta agccgagaaa actaggaaaa acaataaatg
     7321 aaatcgagga aggcgactcg ttatccttaa cagaatctat tgaagataac cagcttttgc
     7381 tgtatttggg attgacgaat gatgcaaatc ctttgtatat ccaacatgac tttgcacaaa
     7441 aaacagaata tcaacagcca atcgttccat cagtgatgct gatggggatc atcacaagtg
     7501 caatttcaaa gcatcttcct ggaccaggat cacatgttgt caatttttct gtcaatttta
     7561 ttgaaccggt tttccattat gaaacactga cttttcagtt tgaggtcata aaagtagata
     7621 agatgaagga tgtggtgacg atttctgtag aagcggagaa tacgaacgaa gatcgtgtgt
     7681 tggatgctgt cgttatggtc caaccgccac aagttcctga attggaaata gcaatcgaaa
     7741 gtgaaggtga attgaatgaa taatccaagt acatcaatgt caggtatcaa tcgtttttat
     7801 gcaaaagttt atggattttt aggtatggga cttgccctta gtgcagtcac ttcttacttg
     7861 gctctttggg tatttccttt gcaattagca acttttatca ataattttcc actaggcttt
     7921 gtgggtctgt ggatcgttga actgatttta gtaatggttc taggcgtaaa agcacaaaag
     7981 aatccgagct tagcaatcgg tggatttatt gcctactctc ttttgaatgg cataacatta
     8041 gcggttactc ttgcatacta cgatattgga acagtaacgc aagctttctt gacagctact
     8101 ttgatgttca tggggatgtc tgttgtcgga gtaacaacaa aaagagatct tagcggggtt
     8161 ggaagagcag gacaggcagc tttaatcgga gtgatcattg cactagtctt gaacatcttc
     8221 attttgagaa gtagcgtagt tgagctgttc atctccctac tgttagtcgt catctttgca
     8281 gggattacag cttatgataa ccaaagcatc cgtaatatct atgtccagac aaacggaaca
     8341 gcacatagtg gaattgctgt attcatggca ttgaagctgt atttggactt tatcaacttg
     8401 ttcttggcgt ttctgagaat ctttggacgg aacaactaaa aaagaaaaaa attcatcagt
     8461 tggggctata ccgaattatt tggtatggtc ccgctttttc ttttcgcatt ccgcaggatt
     8521 ctttgttaga atgaataaga actttacagt aaggagttaa gaagatgaca aaaaataaat
     8581 tattactcgt tgatgggaac agtgtggctt ttcgcgcatt ctttgcgctt cacaattctc
     8641 ttgaacgttt caaaaataga aacggtcttc atacaaatgc aatctatgca ttcaatacga
     8701 tgtttgaaaa tgttatgcaa aaagagcaac cgactcatgt tctggttgcg ttcgatgcag
     8761 gaaaaacgac ttttcggaca gaaatgtatg cggagtacaa aggtggaaga tccaaaacac
     8821 ctggcgaatt caaagaacaa atgccatata tacgtgattt gttgacagga ctaggggtac
     8881 agtattacga attgcccaat tatgaagcag atgatattat cggtactttg gcagaaaaag
     8941 tagacaaaga tcaatttgat gtcgtcgtgc tttcaggtga tcgggatttg actcaattag
     9001 ctagtaaaga agtaaaagtg gatatcactg taaaaggtgt cagcgatatc gaaagctata
     9061 caccagaaca tgtagcggaa aaatatgatg ggttgacgcc aaaacagatt attgacatga
     9121 agggactagc cggagatgcc tctgacaata tccccggtgt gactaaaatc ggtgaaaaaa
     9181 cagctatcaa gctattgaaa caatatggct cagtcgaagg aatttatgaa catatcgatg
     9241 aaatgaagca aagcaaaatg aaagaaaatc tgataaatga caaagagcag gctttcttat
     9301 cgaaaaaatt ggcgaccatc aatacttcag cgcctgtgga tgtctctatc gactcattga
     9361 agtatgaagg gaaaaatctc gataaactcg ttccgtttta taaagaaatg gatttcaatc
     9421 agttcctttc taaattgaat atcgtagaag aagaagtaaa aatggatgat attttgtttg
     9481 aagtcgttca tgaattcaaa gaggaaatgt ttactacgga tatggcgttg tacgtggaaa
     9541 tgatgggcga caattatcat acagaggaaa tcgtcggagt agcgtggggg acagaaaaga
     9601 aaatttatgt cacgaacgat ctatctgtat tcgaaaaccg agcgttccat tcttggatca
     9661 ctgatccgac tcggctgaaa aaagtatatg atgcgaaacg aacttatgtt gctttaaacc
     9721 gctatactgg aaaaacgaaa ggaatcgatt ttgatgtctt gctaggtgca taccttctcg
     9781 atacgaatga aaaaagcaca gatatcgaag gaatagctgc tcattacgga tacaacgata
     9841 tccaatcaga tgaagctgta tacggcaaag gggcgaaaaa aggtttacca gaagaagagg
     9901 aactcttttt tgctcattta gcgcgaaaag tagctgcaat caatgcgttg acaccaaaat
     9961 tgagccaaga actggcagat aaaaaccaag aagatctttt ccggaaaatg gaactgccat
    10021 tgtctatcat tttggcagag atggaaataa gcgggatcaa agtagatgcg actcgcttac
    10081 aagaaatgaa gggcgagttc tctgctcgct taagagagat cgaacaaaaa atctacgaag
    10141 aagccggcga agaattcaat ttgaattcac caaagcaatt aggagtgatc ttatttgaaa
    10201 aaatgggatt acccgttatc aaaaaaacaa aaactggata ttccacagcc gtagatgtac
    10261 tggaacagct tcgtgaacaa gcaccaatcg tagaggatat cctcacctat cgtcaaattg
    10321 caaaaattca atctacctat gtcgagggac tgctgaaagt gatccaatca gatggaaaag
    10381 tgcatacccg ttatgtccag acattgaccc aaacaggacg attaagctct gttgatccta
    10441 atttgcagaa catcccgatt cgattagatg aagggagaaa gatacgtcag gcatttgttc
    10501 ctagagaaaa agactggctg atttattctt ctgactattc acaaatcgag ttacgcgtac
    10561 ttgcgcatat ctcaaatgat gaacatttga aagctgcctt cctagaaggt caagatatcc
    10621 atgccagcac agcgatgcgt gtatttggaa tcgaaaaggc agaggatgtc acaccaaata
    10681 tgcgtaggca ggcaaaagca gtgaattttg gtatcgtgta tggcatctct gattacggac
    10741 tttcacaaaa tctaggaatc agccgaaaag cagcacagca atacatcgat acgtactttg
    10801 aaaaataccc aggtgtcaaa gaatatatgg aaagaatcgt acgcgaagca aaagaccaag
    10861 gctacgtcga aacgctgtat caccgccgtc gatatctgcc agacatcaat tcaagaaatt
    10921 acaatatccg ttcttttgcg gaacgaacag ctatcaatac accaatccaa ggaagtgctg
    10981 ctgatatttt gaaaatcgcg atgatcgaat tggataaacg attaaaagaa acaggtcttc
    11041 aagctacgat gctgttacaa gttcatgatg aacttgtctt cgaggtaccg gaaaaagaac
    11101 ttgaatcatt agataaattg gtgaaggaag ttatggaaca agctgtttct cttcatgtac
    11161 cactgatcac tgacagcagt tggggaaaaa cgtggtacga agcaaaataa acattgttta
    11221 agacagagca atataatgaa ggtgaaaaga aaaaatgcca gaattaccag aagtagaaac
    11281 cgttcgtaaa ggattggaac ggttagtagt aggaaaaacg attcaaaaag ttcaggtctt
    11341 gtggcccaag atcatcgagc aaccagagac acccatcttt gaggcttctc ttgttggcga
    11401 aaccatccaa tccattggac gtagagggaa gtttttaatt tttcatctcg atcattgtga
    11461 actgatctca cacttgcgaa tggaaggaaa atatcaattt acgaaagaaa atacaccaat
    11521 cgataaacat acgcatgtac ttttcttttt tgaggatgga agccaacttc gctacaacga
    11581 tgtacgaaaa tttggtcgta tgacaatcgt cgaaaaagga gcaagtgcaa cttaccgagg
    11641 aatcatgaaa ttgggacctg agcctttgcc agatagcttc ttattagcag atttcgcaaa
    11701 tggactgaaa aagtcccaca aagcaatcaa gccattgctg ctagaccaac gtctagtgac
    11761 tggtctaggt aatatttatg tagatgaagc attatgggaa gcgaagatcc atccagaaca
    11821 accggcgaac acactgcgcc ccaaagaggt agaacaatta cgtctatcga tcattgatgt
    11881 tttggatcgt gcaatagaag ccggaggtac cacgattcgc agttatttga atgcattagg
    11941 agaatcaggc ggatttcaag tagcactgca tgtctatcag caaactggca aaccatgtat
    12001 acgttgtgga acgccgatcg tgaaaacaaa agtggcacaa agggggacgc actattgtcc
    12061 gaattgtcaa aggctaaaaa tcgcaaagta ggatttgttt taggtttgac gggtggaata
    12121 gctaccggta aaagtacggc agccaaagtc tttcagtctc atggatttcc tttgatcgat
    12181 ggcgatgtga ttgccagaga aactgttgag cctggaacgc ttgctctgag gaaaattgta
    12241 tcagtgtttg gccaagaggt attgcagcct gatgggcaac tagatcgagg gaaacttggg
    12301 atgattgttt ttccatcaaa ggaaaaacgg caaaaacttg atcagctatt agatccgttt
    12361 atccgaaaag cgataaaaga tcaaatcgcc tctctaagtt ccaatcatcc tttagtaatt
    12421 gtagatatcc cattgctcta cgaaggtcat tatgaccact atatggatgc agtagctgtc
    12481 gtttatacta cgccagaaac tcagcttcag cgtttgatga aacgtaatca actgacgcta
    12541 gaacaagcgc agcagagaat tagtagtcaa cttccaatcg aagaaaaaaa gcagcgtgcg
    12601 gatattttgt tcgacaacaa tggaacaaaa gaaaaacttg tgggacaaat cgaaaattgg
    12661 ctttttgcta atcgctttct ttgagtatct gtgtcaattt tgctatcctg agtatataga
    12721 aagtgaggga tagcgatggg atcattaaaa aatcaaaagg ataaagtcgt aggtaacgca
    12781 aaagaaaaaa tcggcgagat gctcaataaa gaaaatctaa tcgatgaagg tcgtacgcaa
    12841 tcagaaacag cagaagaaaa agaaaagcag taccaagcaa gaatcgatga aatagaagac
    12901 cagcaggcag aagaagaagc gaagcggcaa gatttgactc aagaacgaaa aaatccgaca
    12961 cctgatcgta gtaaaacacc gctcccagaa agtgaccaca tttccgatga gcaaaaaatc
    13021 gaggaacagc gaatgaaaca ctatacagga atcgaagaga agttataaaa tagtaaaaaa
    13081 tcgagatgtg acttgaagca gatgagaagg tgttcatctg cttcaagttt tttgtgctag
    13141 atgactaaat cagcttttgt gtaagttatt tctcagtaat caaggtgatc agtattggaa
    13201 aattgaaatt tagtaaggac aggggacaca attacgcatg aaagtagaaa aaattgttct
    13261 tatatccgtg ttttttgggt aggacagtcg cgtttgtgat ttgtttcgtg ttataataca
    13321 actagttgta aagaaagaca catcctttat ataaaatgcg atgcaagagg tgagacaaaa
    13381 tgagatgtcc cagatgtcaa cataataatt cacgagtaat cgacagccgt caagcagatg
    13441 atggacgtgc gatccgtcgc cgtcgcgaat gtgagaattg ttcgttccga ttcactacat
    13501 ttgaacgaat tgaagctgct ccgttattag taatcaagaa aaacggggaa cgcgaagagt
    13561 tcaatcgcga taaaatttta agaggactca tccgttcagc agaaaaaaga cctgtagcta
    13621 tggaacaaat ggaacaaatc gtcaaccatg tcgaaaaccg cgtacgcagt ctcggagaaa
    13681 acgaagtatc gacgaatcta attggtgaat atgtgatgga agatttagtt gatttagacg
    13741 aaatcgctta tattcgtttt gcaagtgttt atcgccagtt caaagatatg tctgttttct
    13801 taaaagagtt gcaagatatc gtggataaag caaaagaaaa taataaagag aataataaag
    13861 aaaacaatta agaaacaaaa aggaggcatt tccttggaaa atgcttggaa agagcttcaa
    13921 ccaaaaaata tttaccaagg aagaaaagcg gctccgctga gagaagacag caagaacgcc
    13981 ttgctgtatc tttatcagcc actaatcggc ggagaagcct tgagtatcta tttcaccctg
    14041 ttatcagaaa tctctcaaga atctggcaaa ggtcctgaag ggatgcatgc agatctgctt
    14101 agtagtctag gctgcggtat cccgcaattt tatgatgcaa gaaaaaagct agaaggaatc
    14161 ggcttactgg atgtttattt taaagaagat agtgatctgg gacctcgttt tatttatgag
    14221 ctgttagaac cactcattcc taaagcattt ttcaaagact ctgttctggc ttttcttctt
    14281 ctggaaaaag ttggtgaacg acgattccag aagcttaccc aattctttga acctaaaagg
    14341 catacagcag agggatatca aaaaatcaca aaaaaatttt tagatgttta tcgcttcaat
    14401 gaagcagtgt acgctgctaa tcaagaaaac atcgaatcgc tgaataaatc ttttgaagaa
    14461 tctgaatcag tagcattact tgaagcagat tcgacattcg attggcagtt tttgaatgat
    14521 tggctggcaa aaaaacatat tgcaagtctt gatgatccaa tcatcaaaca agtcgacatg
    14581 tatcatcagc tttacggttt cgatgaattg acacttggcg aactgatcag tcaatcttat
    14641 gactttagtg aacaaaaagt atctgaaaaa gaactgcaaa aagccatttt ggcaatgcag
    14701 agacgtccgg tgaagcaaac aaaagaaaca gttgcggttt ctgaaaacag taatgaaccc
    14761 tcgtcaaacg aacatttgac aaacgcagcg cttcagctga taaaagaagc acagagcatt
    14821 ccaccgatga aatatctgga agcaatcaag tcagaaaaag gcggatatgt ttctaaacaa
    14881 gaaaactggt tactgcaaga tcttgtttct caatcaggtc tttccagtag cgtgatcaat
    14941 gttttgctga attatgtact agtgatcaag aataatgcta gcttaaatgc cagttatgta
    15001 aatacagtgg caaatgaatg ggcacaaaag aaaatcgcca cagcagaaga agcgatccag
    15061 cacattcggc aaataagtaa tcaagcaaaa caatcaaaac aaaagaagca aacaaattat
    15121 caaactggca agcgaaatgt tcgacgggaa aagcttcctg attgggtcaa ccagccaaaa
    15181 gatgaaaaac agatcagcag cgagaaaaaa gcagagattg accgccgttt taaagaatac
    15241 ctggcaaaaa aggagggtga tatctagtgg aagacgtagg caaagaattg aagaggatca
    15301 tcagcaaacg caattatcaa gaaaagtatg ctgaaatgat cgctgaagtc ttaaatgatg
    15361 cggatgttca agcttttcta agcgcacacc aagaagaatt gacacaagcg gatattgaaa
    15421 aaagttacgc taaattgtat gaattcgtgc aagaaaagcg taaatatcaa aaaaacgatc
    15481 caacgatgat cgctcccggc tatgagccga agctgacgtt gaattttcat tatgtggatg
    15541 tcacttatgt accaacggaa gcattgttag cacggcaaag acaagaagaa atcagaagcc
    15601 gagtaaaagc aatggatatg ccaaaagata ttcaatcggc ttcttttgaa aactttgaac
    15661 gtacagatgg acgaggatat gcgagcttag aagccctaga ttttgtagag aaatatactg
    15721 ctgatccaaa agggtatcat aaaggactgt atctagtagg aagttttggt attggaaaaa
    15781 catatttatt aggagcaaca gcgagagaat tagcgattgc tggattcacc acgacgctag
    15841 tccattttcc gacatttgcc gttgagatga aacaggcaat cggaaaagat caagtggcag
    15901 aaaagttaga tgcagtgaaa cgttcaccta ttttgatgat cgatgacatt ggtgcagatg
    15961 cgatgagtag ttggatacga gacgaagtat tcggtgttat ccttcaatat cggatgcagg
    16021 aacagttacc aaccttcttc tcatcgaatt ttacaatgga tgaattggaa cagcatttgt
    16081 ccgtcactca acgtggagat gaagagccgc taaaagcgaa acggataatg gaacgcatac
    16141 gttatttgac aaaagaaatc gaaatgactg gtcgcaaccg acgcaatggt tgaacagttg
    16201 acaagtccaa ctgacatttg acctagtagt aaaagcaggt cagcgaatca ttatatggac
    16261 agaaatgacc ggagacaatc accgcaacaa gtaatgcaga aacttaaacg gaggattttt
    16321 tatgagggct attttttcta gtatatctgt agttggtcta ttttttcttt tagcaggctg
    16381 cggtagcgca gatagcaaca caggttctgt tgattcgatt gatgcgggtc aaacagtaaa
    16441 aacaagcagt acgaaaacgg agagacaaag catatttgtc ggaacgattg aaagtattac
    16501 tcaagtagaa gcagaagaag aaacgcttca gttgaaactc gtcgatgtca tggccaaaga
    16561 agatccagcg aatatgggaa cgtcattcag caatgacgga gtgatcttaa atgctactgc
    16621 tgaaaaaata agcgtggaca taaatgaatt gaaaatcggt gacaaagtcc aatttacatt
    16681 agttgagatg ccgatcatga cgatgtctat cccaccgcaa gttcctggag attcgattat
    16741 tgatgtttca ttgaacaaat aaccaataac attttttcaa aaataagaaa atgttgtttt
    16801 atagaacgaa aaggtatatt ttttaaggag atatgggaat ttttttattc ctagtctcct
    16861 tattttattt ggaaaatgct ataattatct agaaggagtg aagctatgaa ccatttagat
    16921 ttatctcagc gcttagccgc agtgggtgac tttgtccctg aatccgctcg tttagcagac
    16981 attggatcag atcacgctta cttacccgtt gcattgatat taagaggaaa aatcgaatat
    17041 gcagttgctg gtgaagtcgt tcaaggtcct tatgaatcag cacgtaagca agtcaaaaaa
    17101 gatggattag aagaccgtat caccgtacgc cttgcaaatg gtctagatgc tatccagcca
    17161 caagatggta ttacagctgt cacgattgcg ggtatgggag gttcactgat ccgagatatc
    17221 ctagaagctg gcaagttgaa tcaacgctta agcggacaag aacgtttgat ccttcagcca
    17281 aacatcggag agcgtacatt acgatcttgg ctgcaagaaa atcagtataa aatccaatca
    17341 gaaaaaatac ttgaagaaaa taaaaaaata tatgaaatca ttgtagccga aaaagaacaa
    17401 aaaccagtta cttacaatga aatggagctg atgttcgggc catttctgct tgaagagaaa
    17461 agtgagatct tcaaatcaaa atggacaaga gagcttcaac aaagaaaagc tatccgtgaa
    17521 cagctcaaac aagcagctaa tcaagaggca cgtcaaaaac aaatcgagca ggaaatccaa
    17581 atgattgagg aggttttaaa gccatgacaa tcagcggacg cactttcatc caaaagtttg
    17641 aaagctattg tcctcagtgg ctagctgaag aaggagaccc agtcggactg catatcggaa
    17701 cattggataa gccaatccaa cgagtaatga tgacattaga cgttcgtcca gaagtggtcg
    17761 aagaagcaat caagaaaaaa atcgatttgc tgattgctaa acatcctcca atctttcgtc
    17821 ctgtaaaacg gctggttact gatcagccac aagaaaaaat gtatgcagat ttgttgaaac
    17881 atgatattgc cgtctatgca gcacacacga acatggatat tatttgggat ggactgaatg
    17941 actggttttg tgaacttctc ggtattgaag tggagagtta tttagtaaag actcatgaga
    18001 ttcattataa aaaactggcc gtttatgtac ctgtggatca tgcacagaaa atgcgtgaag
    18061 tattagctgc agctggtgct ggtacacaag gtgattatac cggtacaagt tttacctcga
    18121 ttggacacgg tcgatttact ccggaagctg gtgcacaacc agcgattggg aaagtaggga
    18181 agacagaaca agttcaagag gcaaaagttg aggttatttt gccggaaact atcgaaaaac
    18241 aagtgattca agccatgcgg tcagctcatc cttatgagga acctgcttat gatcttttcg
    18301 ctatagatga accagtcgaa atgtttgggt taggacgagt aggtgaattg ccgcaagaaa
    18361 tttctataga ggcatttgta gaacaagtaa aagaagcgtt ccaactagat ggactgagaa
    18421 tagtccagcc aaaaaatgca aaatcctctg tgaagcgcat tgcgatttgc ggtggaagcg
    18481 gtgaaaaatt ttatccgcaa gcaatcgccc aaagagcaga tgtctatatc acgggagata
    18541 tctattatca tacggctcat gatatgcaaa gcgctgggtt gattgcaatt gatcccggtc
    18601 attatattga atcgttatgc aagcaacgat ttatcgagaa atttgaatct tggaaacaag
    18661 aagaaaattg ggatattaac tttttcgttt ctgaaacaaa tacaaaccca tttcaattta
    18721 attaaaagga gagaatacta tgtacgaaaa tcttttgcca cgttttttac gatatgtcaa
    18781 aacagaaaca cgctccgatg caacaagcaa aacgactcca tcgactcaga cacaagttgc
    18841 ttttgcgcaa gaattgaaaa aagaattaga agagttaggc ttagctgatg ttttctataa
    18901 tgaagataac ggattcgtta tgggaacatt accaagcaat gttgatcatg aagtgcgttc
    18961 cattggattt atttcccaca tggatacagc agattttaat gcagtaggag tcaatccaca
    19021 aatcattgaa aactatgatg gcgagtctac gatcgtacta gatgcggaag aacgctatac
    19081 attgaataca aaagattttc caaatttgaa aaattatgaa ggccacacat tgatcacaac
    19141 agatggcagt acattactgg gagcagatga caaatcaggg attgcggaaa tcatgactgc
    19201 aatggagatc ttgatcaata atccttctat ccctcacggt accatcaaag tggcttttgg
    19261 tcctgatgaa gaaatcggtg tcggagcgga taaattcgat gttgagcaat tcgatgtaga
    19321 ttttgcttat acagtcgatg gtggtccttt aggtgagttg cagtatgaaa cgtttagtgc
    19381 tgctcaagca aatattacga tccaagggaa aaatgtacat ccagggacgg caaaagatac
    19441 aatgatcaat gctctacagt tagcaatcga ttttcataat cagcttccag ccgatgaagt
    19501 tccagaaaaa acggaaggat acgaaggttt cttccatttg atggcattgg atgggaatcc
    19561 tgaagaagct tctatgagtt atatcattcg cgatcatgat cgtgaaaaat tcgaagcacg
    19621 caaagcacaa atcacacata tccaagaaga gttgaatcag cgatttgatc aagaacgaat
    19681 caaagtagat atgtatgatc aatattacaa catgaaagaa atcattgaaa aagatatgag
    19741 catcgtggaa ttagcgaaac aagcaatgat cgagcttggt atctctccag tgatcgaacc
    19801 agttcgtgga ggaacagatg gttcaaaaat ctcttacatg ggtattccga cgccaaatct
    19861 atttgccgga ggagaaaata tgcacggacg attcgaattt gtttctttac aagtaatgga
    19921 aaaagcaaca gacgtgattg taaaaattgc agaattgaac acaaagagtt aaaatcgaaa
    19981 aagaacagct tatgacaagg cgttcgtccc aatgatggaa tgtcatctgt ctaaggctaa
    20041 agggcaggac aatggaattg gttgtcccgc cctctttttt gaagagtaaa taggaggtaa
    20101 agaatggaaa agctgctagt tttttataac aaaagttcag ggaaagatga gggtgagaaa
    20161 ttagcacgct ggtttcaaga ttatgtaaaa gaaaagcagc cagaattata tgtagaacta
    20221 gctgaaactg gcccatcgat tgatgatgcg gaattagtca agaaagcaga agccatagaa
    20281 gctgatactt tagtaattat aggtggagac ggcacgattc atcatatcgt ccaagcgttc
    20341 caaaaaacat tatctcaata ccatgtcgga ttacttccag gtggaactgt caacaacctt
    20401 gcacgtgttc ttgagattcc cctcaaaaaa gaagaagctg ctgacgtgat cctggaaggc
    20461 aatgtacgga aaatagacta tggtcaagtc aatgatgaag tcattatctc tacactgaca
    20521 atcggaatct tagcagatac agcagtttgg atcagtcaag aagaaaaaca gcgttacggc
    20581 tcgtggatct ttatcaagaa gttttttaag ttattgctga agaagaaaaa atatcatttg
    20641 gatttagaaa cagacgagga gcattggaaa ggaaaatccc aactgttgac agtcacgatg
    20701 tctaactccg tgggcggttt tacaaatttt gacgactcag cgaccctaga tgatggaaaa
    20761 tttcatgtaa cgatcgtacc ttccttagat attttccgat ttatttttta tttgcctaga
    20821 atcatgagag gcaaattcta tacgatcccc ggcattactt atttcacagc caaacgcatg
    20881 aaaatccaag cgcaggaaaa atctgtgggc actcgaacgg atggagacac gacagatgat
    20941 cttccagttg aattatctgt tgtatcaaaa ggattaacag tttattcacc aaagaaagaa
    21001 gaaaaagctt agaaatagta gttaacagaa agttggaaga agagtaatgg atgtattgtt
    21061 acacaatctt ttgtggtttt accttttcat tgtgaatttt tacttatttt gtctgatggg
    21121 gtatgataaa catcaggcta gaactagcgg ctggcgtgtt cctgagaata atttgatatt
    21181 tgtcagcctg ttaggcggtg gtttgggagg attgatctct cgaaaagttt tccatcataa
    21241 aactcggaaa aagaagttta ctataggatt cgctgcaggt gtacttgttg atttgattct
    21301 catttatttt ttccattaag gagcaggaga tgtcgtctaa aggaacgaaa actaaaatca
    21361 ttctgaatct gcttttgctg attgtgattt ttgccattat ttattattta atcaatcaat
    21421 catttgcaga tatctttgcc gaattgctgt ctaccagtgt tgttgttttg gcagcggctg
    21481 ttatattagg gacagtttac caaatcgctg aagggcgctc aatcaaagaa attgctgaac
    21541 ctttcgcgcc gaattttaca acgaaagatg ggttcttgac ctctagctat attgcgtttt
    21601 accgaatcgt ttcgtttggt acaggaacgc tgatctcgga gatttatttt tataacaaaa
    21661 aaggaatgaa gctatcccag ggagcagggg tgacagcact ccatatgatt atgtacaaac
    21721 tggcagtgat cacttatgca ttagctggct tggtcatcca attctctctt ttttattcta
    21781 atggtccatc aatgatctgg tttatcatcg ctggtatcct tttgacgatc gtgattgttg
    21841 cctttttatt ggcgatgtcc gtcagcttga atgttcaaat attttttgtc agcttatcga
    21901 ataagttatt taaaagtgag cgtttgcgga aatgggtgga tacatgcaat cagcagatct
    21961 actctttaag ggaaacggtc caaaccatta tccaagacag gactgcgcta ttgcggattt
    22021 atctatggaa tatgttaaaa cttgtatttt ggtatgtact tccttatttg attctgattg
    22081 aaaaccatcc taaaatagat tttctactga ccatttcgtt tatcagcttt gcagtagtgc
    22141 tttcaggggt tattcctacg cctgctggta ttggttcctt tgaatttgtc tatttgttat
    22201 tattcagacc agttgttgga acagttgacg cagtttcttc gcttttactg tatcgtttca
    22261 gttcctttat cttgccattt ctctatggat ttgtgtatgt gattgctgaa cggcgaagag
    22321 tgatcaaaga agaaataata gaagtaaaaa aagagaataa agaaaatcaa gggtaggtct
    22381 tttataagat ctaccctttt tatcaaatca aagaaaaacg ttatggataa gataatttta
    22441 cgtttgtttg gctctttttt tatatacata caatcaattt tgttttttta agatttatat
    22501 aagtcgagaa aaaaagtcaa aaaaccatgt gtatattttg accaaaattg accaaagcga
    22561 tataatagtt tttgtaaaga tcaaagaaat aagtgatctt acctttaatt aggagggtat
    22621 acctgtatga atattgagaa aatgactacg acgcttcaag aagcgattgc tgaagctcaa
    22681 cagattgcgg tcactcgaaa acatcaagat atcgacattg cccatgtatg gaaaatcttt
    22741 ttacaaccta atcattttgg tcgaaatttc tatacggatg ccggattgga tgtcgaatct
    22801 tttgaacatg aaattgatcg tctactggat gagtatccag ttgtttctgg tggaaacgtc
    22861 caatatggac aaaacctcag ccagaatttc tttcgattat tgaatgaagc agaccaaata
    22921 cgtgaaagct tcggcgacga gtttctttca acggaagtcg ttattttagc gttgatgaaa
    22981 ttaaaaaatt atccgttaac tgtttatttg aataaaaacg gattatctga aaaagaattg
    23041 cgtaaaaaca tcgaagagat gagaggagga gaaagagtga cctcacaaaa ccaagaagaa
    23101 caatacaaag cattagaaaa atacggcgtt gatttagtac agcaagtcaa aagcggaaaa
    23161 atggatccaa tcatcggtcg tgatgaagaa atccgtgatg tcatccgtat tctttcacga
    23221 aaaacaaaaa ataatcctgt tctgatagga gaacccggtg tagggaaaac ggcaatcgtt
    23281 gaagggcttg ctcaacgtat cgtacgcaaa gatgtgcctg aaaatttaaa agataaaacg
    23341 attttttcat tagatatggg tgcattgatc gctggagcaa aattccgtgg tgaatttgaa
    23401 gaaagactaa aagcagtctt gaaagaagtc aaaaaaagtg atggacgtat cctcttgttc
    23461 atcgatgaaa tccacaatat cgtaggggca ggaaaaacag aaggaagtat ggatgccgga
    23521 aatctgttga aaccaatgct tgctcgcggt gaattacatt tgatcggtgc cacaacttta
    23581 gatgagtacc gtcaatatat ggaaaaagac aaagcgttgg aacgtcgctt tcaaaaggta
    23641 ttagtcaaag aaccaacagt ggaagatacg atcagtattc tgcgtggatt aaaagaacgt
    23701 tttgagatcc accatggtgt taacatccat gacaacgcat tagtagcagc agctacttta
    23761 tctgaccgtt atattactga tcggttctta ccggacaaag caatcgattt gatcgatgaa
    23821 gccagtgcaa cgattcgggt agaaatgaac tctatgccaa cggaattaga tcaagttact
    23881 agacgtttga tgcagttgga aatcgaagaa gctgcactga aaaaagaatc agatgatgca
    23941 agtaaaaaac gcttgaaaaa tttgcaagaa gaattagctg aacttcgtga agaagcaaat
    24001 gcgatgaaga tgcagtggga aacagaaaaa gaagaagtca attctgtttc agcaaaacgt
    24061 gctgaaatcg ataaagcaaa acacgaatta gaagatgcag aaaataacta tgatttagaa
    24121 cgcgctgctg tattgcgtca tggtacgata cctcaattag aaaaagaatt gaaggaatta
    24181 gaagctaaag caaaagacag cgagattaag atggttcaag agtccgttac cgaaaatgag
    24241 attgctcaag tcgttggcag attaacgggt atccccgtaa cgaaacttgt agaaggtgaa
    24301 cgagaaaaac taattaaatt gaatgaaaca cttcataaac gagtgattgg tcaagatgaa
    24361 gcagttgatg cggtcagtga cgcggtaatt cgttcacggg ctggattgca agacccaaat
    24421 cgtcctctag gttctttcct tttcctagga ccaactggtg tcggaaaaac cgaattagcg
    24481 aaagcgttag ctgagaactt gtttgactca gaagatcata tggtacggat cgatatgagt
    24541 gaatatatgg aaaaacatgc tgtgtctcgt ctagtcggag ctcctccagg ctatgtaggt
    24601 tacgaagaag gtggtcaatt aacggaagct gtcagacgaa atccttatac aatcgtcttg
    24661 ttggatgaaa tcgaaaaagc gcatcccgat gtcttcaata tcttgttaca agtcctagat
    24721 gacggacgct tgactgattc aaaagggcga gtagtagatt tcaaaaatac ggttctgatc
    24781 atgaccagca atatcggttc acagttactg ctggaaggcg tgactgctga tggaacgatt
    24841 ccagaagcag tggctgaaca agtcaacacg ctattacgtg gaaacttcaa accagaattc
    24901 ttgaaccgaa tcgacgacac gattttattt acaccattga gcctagacaa cgtgaaagga
    24961 atcgttgaca aaatggtggc gcaattggca caacgtctgg aacatcaaga aatcctgctg
    25021 acaatcagtg atgaagccaa aacgtggatt gctgaaaatg cttatgaacc agcatatggt
    25081 gcaagaccat tgaaacgatt tatcactaag gaagtagaaa caccactagc taaagaaatc
    25141 gttgcaggac atgttatgcc taaatcaaaa gtaacgatta ctttacttga tggacaactt
    25201 catttcaaaa cagaagagct agaagaaatc gtctaatctt ccattgagac tataaaagaa
    25261 aaaaggggtc tgacacaact agtgtcagac ctccttt
//
LOCUS       DACGLE010000036        24336 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0534
            SAMEA3304449-rid9560453.denovo.037, whole genome shotgun sequence.
ACCESSION   DACGLE010000036 DACGLE010000000
VERSION     DACGLE010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304449
            Sequence Read Archive: ERR985064
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24336)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24336)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702225.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702225.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 20:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,045
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 27 of 67
            Pseudo Genes (incomplete)         :: 25 of 67
            Pseudo Genes (internal stop)      :: 19 of 67
            Pseudo Genes (multiple problems)  :: 4 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24336
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304449-rid9560453.denovo.037"
                     /strain="VREN0534"
                     /db_xref="taxon:1352"
     gene            complement(132..2477)
                     /locus_tag="IXT20_001585"
     CDS             complement(132..2477)
                     /locus_tag="IXT20_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HAQ4860033.1"
                     /translation="MPRKNTRKKQNRRKKEKWFVPKIIFRVFQSLTVFITVLLLLFAA
                     LGIGIGAGYFAYLVEDTQLPTKKELQTELGNITETSKIVYADNTEISKIQTDLMRTTI
                     SSDKISPLLKTAIISTEDEYFDKHQGYVPKAVLRALVSEATGIGSSGGSTLTQQLVKQ
                     QILTDETTFKRKANEIVLSAQVEKHFSKNEIITTYLNVSPFGRNNKGQNIAGVQEAAR
                     GIFGVDAKDVTLPQAAYIAGLPQSPITYSPYTNTGALKEDLSAGLARKDFVLFSMYRE
                     GQITKEQYEEAKAYDLTKDFLPQQIAEQTDREFLYYTVMNEATRIIAQQLAEKDNADM
                     SDSSVSEAYYQKAQQTIQNKGYTIHSTIDKDIYAALQTGVENYGYMLDDGAGSQVETG
                     NVLMDNRTGRIYGFVGGRNYSQNQNNHAFDTERQAGSSIKPVLVYGPAIDMGLVGSES
                     RVSDYATTWQEGKNAGEKIVNATNEGSNTFQTVRESLEWSNNIPAYHLYQDVLNSGGS
                     KQYAYEHYLAKMNYPANDNWGVESAPLGTVDVTTLQQTNGFQALANGGVYEEGYIIDS
                     ITDNAGNVIYKHESNSVRIYSEATASIMNDMMRSVINEKITTPFKDVISSLNGNLGNA
                     DWVGKTGSTNEYRDSWLIVSTPSITISSWAGHDDNTGMDSQARIRSANYLANLINQAY
                     QANPEIFGTDEKFDLSSDVMKKKVSGFTGQTAGKVTVNKKSIQTPGKTVESLWAKNGP
                     KKSAFKFGVGGTDENYTDYWNTASAYARANPETKESDDKDD"
     gene            2756..3658
                     /locus_tag="IXT20_001586"
     CDS             2756..3658
                     /locus_tag="IXT20_001586"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HAQ4860034.1"
                     /translation="MLLGSHVSMSGKKMLLGAAEEAASYGSSTFMIYTGAPQNTRRKA
                     IEEMNIDAGKAFMAENGLTNIVVHAPYIINLGNTIKPEMFPFAVQFLREEIVRAEALG
                     ATQITLHPGAHVGAGAEAGIARIIEGLNEVLTKEQTAQIALETMAGKGTEIGRTFEEL
                     AQIIDGVKLNEKLSVTFDTCHTNDAGYNIKEDFSGVMEEFDKVIGLDRLKVLHINDSK
                     NPQGSHKDRHANIGFGTIGFDTLNKIVHDPMFDNISKILETPYVGKDKKKAYAPYGKE
                     ISMFKAQAFDPEVFPELLAEEVTL"
     gene            3869..5644
                     /locus_tag="IXT20_001587"
     CDS             3869..5644
                     /locus_tag="IXT20_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyltransferase"
                     /protein_id="HAQ4860035.1"
                     /translation="MLNILNPLIDQLKKKHTYFILGIAVLFTIIRLYLYSKAIYGTDM
                     SATYDDQLLIHYAQNILDGKWLGEYTTTTLSKGISYSLFLVLAHKLHLSYAVLFGMFS
                     IFASILVALAFKPIVKNKYLLLSIYFYFLFSPISFTHEYSTRTYRNALVIPAVEIIIA
                     CLLAIYYRKYGSSRKLLPWFLGLSVAFPFFWFIREDSIWLLPFTVVALGISFVQILLG
                     QDFSFKEVKKLKKRLFLLSKREWLKLSLFLLPIVGFSIVYQWIEYKNETTYGIATIND
                     RSSGEFGRLMKQLIRIDDETNLNKENSEIWVSHAALNKALAASPTFHTVSTRIDELYT
                     THAWTQGGKVKELPGDIIFWALRDVVSESGYYQNNAKETNDFWKKVNQELEEAYHKGT
                     IKKKKEFYLMGSGDGKTMSDLPIVFDFLQSGYAYAWRYKDFHQGGNYSFGTQEQVKQA
                     QELLNLPLMENWIDSNEPRTPNFQLTKSAKVANVIIKIYQVLAIPVLIASIAGILLMI
                     LGTVFAKADRAYYSSSLIVVTGLILTQLVFLFGVSWFCSYAPEKKDYFLSVYTGAGVP
                     LTQFIVVTSMLVFSKLNLKKALSSK"
     gene            complement(5710..7296)
                     /locus_tag="IXT20_001588"
     CDS             complement(5710..7296)
                     /locus_tag="IXT20_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HAQ4860036.1"
                     /translation="MNNQSNKQFHKHRLIEHTNGLSLAEVNGTVDVPKGKSFFKTLLA
                     YSGPGALVAVGYMDPGNWSTSITGGQNFQYLLMSVILMSSLIAMLLQYMAAKLGIVTQ
                     MDLAQAIRARTSKTLGVILWILTELAIMATDIAEVIGAAIALYLLFDIPLVIAVFITV
                     LDVFVLLLLTKVGFRKIEALVVCLIFVILFVFVYQVGLSNPDWHSLFAGFIPNSHTFS
                     DSPVIGGETPLTGALGIIGATVMPHNLYLHSAVSQTRKIDHQNEEDVAQAVRFSTWDS
                     NIQLSMAFLVNALLLIMGVAVFKTGSVQDPSFFGLYEALSDPSAMSNGILIAVAKSGI
                     LSTLFAVALLASGQNSTITGTLTGQVIMEGFIHMRIPIWLRRLVTRLLSVIPVLICVL
                     FTSTKGTIEEHAALNNLMNESQVFLAFALPFSMIPLLMMTNSQAEMGSRFKNSFIVKL
                     LGWFSVISLTYLNLRGLPGQIESFFGEQATSAQLALADHIAYVIIVLVLLLLAWTIGD
                     LYKGNKKYEQLLQETVEEIE"
     gene            7547..7771
                     /locus_tag="IXT20_001589"
     CDS             7547..7771
                     /locus_tag="IXT20_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YneF family protein"
                     /protein_id="HAQ4860037.1"
                     /translation="MVSTGIVVLIAIIALLIGAVGGFFLARKYMKDYLEKNPPVNEDM
                     LRSMMMSMGQKPSEKKIRQMMQQMKNQGKK"
     gene            7913..9664
                     /locus_tag="IXT20_001590"
     CDS             7913..9664
                     /locus_tag="IXT20_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ4860038.1"
                     /translation="MSIFRKLGWFFRQEKKHYLIGIFSLFAVALVQLVPPKVIGIIID
                     EIADKNLSIKIISWWILLLILAAFLQYLFRYIWRTNIWGSAARLERELRQQLFKHFTE
                     MDNLFYQKYRTGDLMAHATNDLNAIQNVAGAGILTFADSVITGGTTIIAMILFIDWRL
                     TLIALIPLPLLAVTSRVLGSKLHDAFRDSQEAFSSINDKTQESITGIKVIKTFGQEKE
                     DLADFEEKIEDAIIKNKRVNFLDSLFDPFITLIIGLSYVATIILGGKYVLEGTISIGQ
                     MVSFISYIGMLVWPMFAIGRLFNVLERGNASYDRVNELLHEKTHIVEQLGAVQTPAKG
                     ELNVAISSFNYPKEESPALEQIKFAIQEGETLGIVGKTGAGKTTIIKLLMREYDDYQG
                     KIQFGGRSIKEYSLNALLGSIGYVPQDHFLFSMTIRDNIRFSNPKLSEEEVEHAASLA
                     FINNEIKELPKGYDTLVGERGVSLSGGQKQRISIARALIKNPELLILDDALSAVDAKT
                     EEAILHNLKEIRKEKTTIIAAHRLSSVMHAKEIIVVDQGKIIERGTHHELLALGGWYE
                     RMWEKQQLEAKIEGGEA"
     gene            9664..11448
                     /locus_tag="IXT20_001591"
     CDS             9664..11448
                     /locus_tag="IXT20_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ4860039.1"
                     /translation="MDENYQSEWTKSIPLKEQKKIIKRLMKFAKPFRRTFIVAILFAF
                     ALSVINVLLPRIIQTFMDDHLAKQSATTQVILFFAGVYLFGVIVKSIIWFFQWYLYSM
                     ASLKTYQYVRVRLFEKLHTLGMRYFDQTPAGSTVSRVTNDTETLFEFWYVFLMVLTGI
                     FAVVSSFVAMFQINGEIALWTLVFLPILGIVIWYYQKFSSRIYRNMREKLSQLNTKLN
                     ESISGMRIIQQFRQEARLAKEFEQVNNEYLDTRYAMIKTNSILLSPIINFLYALAIAL
                     ALTLFGIDALHSPVEVGLIYAFTTYVQEFFNPMSQMMDFLSTFTDGMVAGSRILKIMD
                     TEEYAPQQNPGADQAITSGKIEFRNVSFSYDGEHNVLNNISFVANPGETVALVGHTGS
                     GKSSIINVLMRFYEFYEGQILIDDHDIREYPMEELRKKMGLVLQDAFLFYGDIAGNIR
                     MMNPAITDEQIRHAAEFVQANKFIDELPKGYHSKVIEHGASYSSGQRQLITFARTIVT
                     DPKILVLDEATANIDTETEGLIQEGLAKMRQGRTTIAIAHRLSTIRDAELILVLDKGH
                     IVERGNHETLLAQNGLYADMYKLQSSGV"
     gene            11783..12635
                     /locus_tag="IXT20_001592"
                     /pseudo
     CDS             11783..12635
                     /locus_tag="IXT20_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289170.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
     gene            12732..14618
                     /locus_tag="IXT20_001593"
     CDS             12732..14618
                     /locus_tag="IXT20_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ4860040.1"
                     /translation="MGKYQELARKIVENVGGKENINGLTHCITRLRFKLKNEEKANDE
                     ILKNMDGIVTVMRAGGQYQVVIGNHVPVVFEEVIKAGNLTFDEAVSTEKMRPFDMLID
                     GISGCFQPFLAILAADGMIRGLTAFLVFLGAFDRGSGTFVMFDNIGDSVFQFMPVIIG
                     LTAARKFKVNEFVGMLLGAALMNPSLSLEALSGAAEAPLTTIFSGTIFEAPIYQTVFG
                     IPWIARNYASSVIPIIFIVLLASQIQKPIKKLVPEMIANFFVPFFTVLITMPLGFLLV
                     GPVFTFATDILMAGFETLLALSPVIYGAIVGFFWQILVMFGLHWAIVPMGLMQFSVNG
                     WQNIMTPVAVVSFGQTAALTALYFKLRNPKDKAIAIPAIVSGIVGITEPAIYGFTLPR
                     KKIFIFTCVGGALGGAYSGLMNLTSWNQGGLGIFTIPNYIRPDGDLTDVINVLIGIAI
                     AMVVSFALTFFFWKEEADETDDRQKRSGKEIVKTPIQGQIAPLNAAKDAAFAQGTLGR
                     GILIYPEKGEVRAPFDGTVMSLFPTKHAIGMVSEAGLELLIHVGLDTVQLEGKYFESL
                     VQQGERVKQGDLLLRFDKKKIEEEGYSLETPVIVINYMDYLDILENQGETVGPDDALI
                     TALT"
     gene            complement(14659..15561)
                     /locus_tag="IXT20_001594"
     CDS             complement(14659..15561)
                     /locus_tag="IXT20_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAQ4860041.1"
                     /translation="MLLREKIKEISFSPSENEVIQFLLSDSPALGEMTIQEIAERCYV
                     HPSTLIRVAKKMGFDGWVSFRNAFLAETAYLEGNFQDVDANLPFSTTDGIMTIANKMA
                     SLEQMTIQDTLSLIHHDQLQKAKQLMLQAKKIVIFASNSNTLISQDFMLKMKRIKMDV
                     TIVTTFGESFYEAYNCTSDMCGILISYTGENNVILRTAEILKQNHVPILALTSIGDST
                     LSSMSTAALRLTTRERLYSKIGNFTINTSICYLLDVLYSCVFAENYQQNLVHLISVGQ
                     KFDKRPISSAIMKEPFNFENKEVD"
     gene            15791..17194
                     /locus_tag="IXT20_001595"
     CDS             15791..17194
                     /locus_tag="IXT20_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HAQ4860042.1"
                     /translation="MAKGIKIATIGGGSSYTPELMEGFIKRYEELPIREIWLVDVEEG
                     KEKLEIVSQMSQRMWDASPYEVKIYATLDREEALKDADFVTTQFRVGQLDARIKDERI
                     PSYYGMIGQETNGAGGIFKAFRTVPVVLAIVEDMKRLCPNAWLINFANPSGMVTEAVI
                     RYGKWDKVIGLCNVPVMAMMTEPTMLGKKKEELIYKFAGLNHFHWHKVADNKGNDRTA
                     ELIDRLYDEDNGLPKNIFDIPFFKEQLKQMNMIPCGYHRYYYREEEMLEHALEEYQTI
                     GTRAQQVKETEAELFELYKDPELDHKPEQLQKRGGAYYSDAACETIASIYGNKETQIV
                     VSTKNDGAVPDLPADCVVEVTAYVGAQGARNVAFGSLPAAERGWLQVMKNMELLTIEA
                     AVTGDYGTALQAFTINPLIRSGKTAKRIMDELFIAHQQYLPQFKETISRLKAEGIEVQ
                     DELARELSSNTHVMEEV"
     gene            17194..18627
                     /gene="ascB"
                     /locus_tag="IXT20_001596"
     CDS             17194..18627
                     /gene="ascB"
                     /locus_tag="IXT20_001596"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HAQ4860043.1"
                     /translation="MGFRKDFLWGGATAANQCEGGYDEGGRGLANVDVVPIGEDRFPV
                     ITGKKKMFEFDENHFYPAKIAIDMYHHYKEDIALFAEMGFKTYRMSIAWSRIFPMGDE
                     KEPNESGLKFYEDVFKECQKYGIEPLVTITHFDCPMHLIKEYGAWRSRELVGFYENLC
                     RVIFNRYKGLVKYWLTFNEINMILHAPFMGAGLYFEEGENEEQIKYQAAHHELVASAI
                     ATKIAHEVDPENKVGCMLAAGAYYPYTCNPEDVWASRKSDRENYFFIDVQSRGEYPAY
                     ALKEMERNGIQIKMEPGDAALLKEHTVDFISFSYYSSRVSTTDNELLEQTAGNIFASV
                     KNPYLEASEWGWQIDPLGLRITMNDIYDRYQKPLFIVENGLGAVDTPDENGYVEDDYR
                     IDYLAAHIQAMKDAVELDGVDLLGYTTWGCIDLVSAGTGEMKKRYGFIYVDRDNEGNG
                     TLARSKKKSFNWYKKVIDSNGENLSND"
     gene            18772..19698
                     /gene="trxB"
                     /locus_tag="IXT20_001597"
     CDS             18772..19698
                     /gene="trxB"
                     /locus_tag="IXT20_001597"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HAQ4860044.1"
                     /translation="MYDVIVIGAGPAGMTAALYASRSNLSVLMIEQGAPGGQMNNTAE
                     IENYPGFDSIMGPDLAYKMYEGVSRFGTENAYGIVQDIKDHGSHKEVICEDKTYEGKT
                     VIIATGCIHRKLGVPGEEEFAGRGVSYCAVCDGAFFRNKRLVVIGGGDSAVEEAIYLT
                     QFASEVVIVHRRDELRAQKIIQDRAFANEKISFMWDTVVDEIVGNEMVVTGVKARNVK
                     TGNEFDLAADGVFIYVGLDPLTAPFREAGLTNEAGWIPTDNEMKTNIPGVFAIGDVRE
                     KDLRQITTAVGDGGIAGQQVYKYLESLEETTA"
     gene            19911..21638
                     /locus_tag="IXT20_001598"
     CDS             19911..21638
                     /locus_tag="IXT20_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HAQ4860045.1"
                     /translation="MSWEQVYEQWVHEENLEENLKKQLNDLGEDPEKLEDAFYAPLEF
                     GTAGMRGILGPGINRMNIYTVRQATEGLARFMDTQGEETKRRGVAIAYDSRHQSPEFA
                     MEAAKTLAHHNIPSYVFESLRPTPELSFAVRHLKTFAGIMVTASHNPAAYNGYKVYGE
                     DGGQMPPADADALTKYVREVSNPLKVEVLSDDEAEHSGLITIIGEEVDAAYLEEIKAV
                     TIDHELVETMGKDLKLVYTPLHGTGKMLGERALKQAGFEQFVLVPEQAVADPDFTTVK
                     SPNPEEHSAFEYAMRLGEKEGADLLIATDPDADRLGAAVRLPNGSYEVLTGNQIGALF
                     AQYILEAHQSRGTLPENAAVLKSIVSSELPTAIAEHYGATMFNVLTGFKFIAEKIQQF
                     EEDHSHTFMFGFEESYGYLVKPFVRDKDAIQALLLLAEVAAYYKKQGKTLYDGLQTIF
                     ETYGYYAEKTISVTMSGQEGSAKISALMEKFREEAPTEFAGAKVIQTEDFKELTRTDR
                     EGNVEKLTTPPSDVLKYTLEENGWIAIRPSGTEPKIKFYIGVKGNSSEDAAEKVAAYE
                     AIIKKMTEE"
     gene            21711..22004
                     /locus_tag="IXT20_001599"
     CDS             21711..22004
                     /locus_tag="IXT20_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HAQ4860046.1"
                     /translation="MPDTQKTKVEKVSQLFKMLSDPTRLKILLYLKDGEQNVTAITQA
                     VEMEQSAVSHQLRLLRENHVVKSHREGKAILYSLDDSHVLDILNQTLKHVEHQ"
     gene            complement(22098..22958)
                     /locus_tag="IXT20_001600"
     CDS             complement(22098..22958)
                     /locus_tag="IXT20_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase/pyrophosphorylase"
                     /protein_id="HAQ4860047.1"
                     /translation="MSSDSTEIITFFVISDSAGETATKLAQATMAQYPTVEFNLFRRT
                     FVTDEKTLMQALQDALKEKALVLHTLINQELIEMTRSFCEEHQLFSFDVMTPPISEIE
                     RLTGIKPIRQPGALHLLNENYFKRIKAMEFAVKYDDGKDPRGFLEADVVLLGVSRTSK
                     TPLSLFLANKNLKVANLPLIPQAHIPKQLWEIDPKKIVGLTNNPDILNNIRKERMRSY
                     GLNPDTAYSDIEKIRAELDFANDLYEKLGCVVINVASLSIEETASLILNALDLEDHSY
                     YGTETTEETD"
     gene            23050..23478
                     /locus_tag="IXT20_001601"
     CDS             23050..23478
                     /locus_tag="IXT20_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HAQ4860048.1"
                     /translation="MKKQTLIEQSLETLKNNGFKYTKKREALLTYLVKRNRYVSAKEV
                     FDYMNQEFKGVSYDTIYRNLHDFAEIDLLEETDLNGEMKFRFHCCQGEIGHHHHFICT
                     VCGTTKEIHMCPMNYFEEQLGGCTIEGHRFEIFGRCENCQ"
     gene            23640..23816
                     /locus_tag="IXT20_001602"
     CDS             23640..23816
                     /locus_tag="IXT20_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010754017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HAQ4860049.1"
                     /translation="MSKTVVRKNESLDDALRRFKRSVSKAGTLQESRKREFYEKPSVR
                     RKKKSEAARKRKKF"
     gene            23843..24289
                     /locus_tag="IXT20_001603"
     CDS             23843..24289
                     /locus_tag="IXT20_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HAQ4860050.1"
                     /translation="MSLLSRLNDDMKTAMKAKDKESLQVIRMIKSSIQNEQIKEGHDL
                     TEEEELTVLSREMKQRRDSLHEFEEAGRDDLAEKVKSEIVIVEKYMPEQLSDEEIRQL
                     VQEAITQTGASSMKEFGKVMGAIMPKVKGKADGNQVNAIVKELLQS"
BASE COUNT         7708 a         4352 c         5186 g         7090 t
ORIGIN      
        1 gtttttcaca tttttacata aatttgtctt aatatcgtag atgtaagctt ttgaacacca
       61 tttattcgga aaaagggagc tgtgacaaga tttttgtcgc agctcccttt aattctgatt
      121 aatctattag atcaatcatc tttatcgtct gattctttgg tctctggatt cgctctagca
      181 taagctgaag cggtgttcca gtaatctgta tagttttcat cagtaccgcc tacgccgaat
      241 ttaaaggcgc ttttcttcgg accatttttt gcccaaagtg actccactgt ttttcctggc
      301 gtttggatgg attttttatt gacagtaact tttccagctg tttgtcctgt gaacccagat
      361 acttttttct tcatcacatc ggatgataag tcaaatttct catcagtgcc gaaaatctca
      421 gggtttgctt ggtaagcttg attgattaga ttggcaagat aattagccga acgtattctt
      481 gcttggctgt ccattccagt attgtcatcg tgtcctgccc aactactgat agtgatagat
      541 ggagttgaga cgatcagcca tgaatctcgg tattcattgg tagaaccagt ttttccgacc
      601 caatctgcat ttcctagatt tccgtttaag cttgagatga catctttgaa tggtgtagtg
      661 attttttcat tgatcacact acgcatcatg tcattcatga tcgatgccgt tgcttcggaa
      721 taaatacgga cagaattact ttcatgttta taaatcacat tacctgcatt atctgtgatg
      781 gaatcaatga tatagccttc ttcgtagact ccaccatttg ctaaggcctg gaatccattt
      841 gtctgttgaa gcgtcgttac atctactgtc cctaaaggag ccgattcaac gccccaatta
      901 tcatttgctg ggtagttcat cttcgccaag tagtgctcgt aagcgtattg tttcgatcca
      961 ccggaattca acacatcttg atataagtga taagctggga tattgttgga ccattccagt
     1021 gactcacgga cggtttggaa ggtgttcgac ccttcattgg tcgcattgac gattttctca
     1081 ccagcatttt ttccttcttg ccaagtcgtt gcataatctg atacacggga ttctgatccg
     1141 acaagtccca tatcgattgc aggaccgtaa acaagaaccg gtttgatgga tgaaccagct
     1201 tgacgttctg tatcaaaggc gtggttattt tgattctggc tgtaattccg accacccacg
     1261 aagccataga tacgtccggt tctgttatcc atcaatacat ttccagtctc cacttggcta
     1321 ccagcaccat catctaacat gtagccataa ttttctacgc cggtttgtaa tgcagcatag
     1381 atatctttat cgatcgtcga atggatcgta tatcctttgt tttggatcgt ttgctgtgct
     1441 ttttgataat aagcttcact gaccgatgaa tctgacatat cagcattatc tttttctgcc
     1501 aattgttgtg caataatcct tgttgcttcg ttcatcacgg tataatacaa gaattctctg
     1561 tcagtctgtt ccgcgatttg ttgtgggaga aagtcttttg taagatcata ggctttcgct
     1621 tcttcgtatt gttctttcgt aatttgtcct tcacggtaca tactgaataa aacaaaatct
     1681 tttctggcaa gaccggcaga aagatcttct tttagcgcgc ctgtattcgt gtatggacta
     1741 tacgtgatag ggctttgagg caaaccagcg atataagcag cttgtggcag agtcacatcc
     1801 tttgcatcta caccaaagat tcctctagca gcttcctgga ctccagcaat attttgcccc
     1861 ttattattcc gaccaaatgg tgagacattc aaatacgtag taatgatctc attttttgaa
     1921 aagtgttttt ctacttgtgc agaaagaacg atctcgtttg ctttacgttt gaatgttgtt
     1981 tcatcggtta ggatctgttg tttgactaat tgctgtgtca aagtggatcc tccagacgag
     2041 ccgatgccag tcgcttctga aactagtgct cgtaaaacag ctttagggac ataaccttga
     2101 tgtttatcga agtattcatc ttctgtactg ataatcgcgg ttttcaataa aggcgaaatc
     2161 ttatcagatg aaatagtcgt ccgcatcaga tctgtctgga tttttgatat ctctgtatta
     2221 tctgcataaa caatctttga cgtttccgta atgtttccta gttctgtctg cagttctttt
     2281 ttagtcggca attgtgtgtc ttcgacaaga taggcaaagt agccagcacc tattccgata
     2341 cctagagctg cgaacaacaa aagaagaaca gtaataaaaa cagtcaatga ttgaaacact
     2401 ctaaaaataa tcttaggaac aaaccatttt tctttttttc ttctattctg tttttttcga
     2461 gtatttttgc gtggcaaaag ggtcacctca actaatggtt ttatttcgct aattatagca
     2521 aataatagga caaaaaaaca gacaaggctg tttttgacag gaatttcttt ttcgaatgag
     2581 ataaaacgtg aggaaatgat cttttttatg aaagagttct taagcttttc tttagggaaa
     2641 gtttcagtta ctatggagaa cagggcaaag aaaaacaaac cattttcttt gtacatcatc
     2701 ttatcaatat acaggaattg ttcgaaacaa ttgcctttca aggaggaatc catccatgtt
     2761 attaggctca catgtcagca tgagcggaaa gaaaatgctg ttaggcgcgg cagaagaagc
     2821 agccagttac ggatcttcga cctttatgat ctatacaggc gctcctcaaa atactagaag
     2881 aaaagctatc gaagagatga atattgatgc cggaaaagca tttatggcag aaaacggact
     2941 aacaaatatc gtcgttcatg cgccttatat cattaatttg ggaaatacga tcaaaccaga
     3001 aatgtttcca tttgctgtac aatttctgag agaagagatt gtccgtgcgg aagctttagg
     3061 tgctactcaa atcacccttc acccaggcgc tcatgtagga gcaggggcag aagctgggat
     3121 tgctagaatt attgaggggt tgaatgaagt tttgacaaaa gaacagacgg cacagatcgc
     3181 tttggagacg atggcaggga aaggtacgga aatcggtcga acatttgagg aattagcgca
     3241 aatcatcgat ggagtaaaat tgaatgaaaa attatccgtt acttttgata catgtcatac
     3301 gaatgatgcc ggatataata taaaagaaga tttttcaggt gtaatggaag agtttgataa
     3361 ggtcatcgga ttagatcggc ttaaagtact gcatatcaat gattctaaaa atccacaagg
     3421 ttctcataaa gatcggcatg ccaatatcgg ttttggaaca attggatttg acacattgaa
     3481 caaaatcgtc catgatccaa tgtttgacaa catttcgaaa atattagaaa ccccatatgt
     3541 cggtaaagat aaaaagaaag cttacgcacc atatggaaaa gaaatttcca tgttcaaagc
     3601 gcaagcattc gatcccgaag tgttccctga gttattagca gaagaagtca cactttaagt
     3661 tttctacctg acagcaacaa aaaaggattg ggcagtctgc cttgtccttt tttgtttaag
     3721 aaacaaagga gtgctttttg cattcgtttt cgccatgctc cttttgcccc ctctttttaa
     3781 attcagacaa acagggtata ctgatagaaa agtaaaaaac gaaaagtttc acaaacaagt
     3841 agcaaaacgt gattgaaagg acgaaaagat gctgaacatt ttaaacccct tgatcgacca
     3901 actaaaaaag aaacatacat actttattct tggcatagcc gtattattta caattattcg
     3961 gttgtattta tatagtaaag caatctatgg aacagatatg tcggcgacct atgatgatca
     4021 gctgctgatt cactatgctc aaaatatttt ggatggtaaa tggctagggg agtatacgac
     4081 tacaacactt tccaaaggaa tctcctacag cttattttta gtacttgcac ataaactgca
     4141 tctttcttat gcagttttgt tcggcatgtt cagtattttt gccagtatat tggttgcact
     4201 cgcgtttaaa ccgattgtga agaacaagta tcttctttta agtatctatt tttatttttt
     4261 gttttctccg attagtttta cacatgaata ttccactcga acatatcgta atgccctcgt
     4321 gattccggct gttgagatca ttatcgcttg tttattagct atttattacc gcaaatacgg
     4381 ttctagcaga aaattgctgc catggttttt agggctttct gtcgcttttc ctttcttttg
     4441 gttcattcgc gaagattcta tctggcttct tccttttact gtagtagctt tgggtatcag
     4501 tttcgtccag atcttgctag ggcaagactt ttctttcaaa gaagtaaaga agttaaaaaa
     4561 acgactcttt cttctctcga aacgggaatg gctgaaactt tcattgtttt tacttcctat
     4621 agttggcttt tccatcgtct atcagtggat cgaatataaa aatgagacaa catacggtat
     4681 tgcaaccatc aatgaccgtt ccagtggaga atttggacgt ttgatgaaac aactgatccg
     4741 aatagatgat gaaacgaact tgaataaaga aaacagtgag atatgggtct cacatgctgc
     4801 actaaataaa gcattagccg catctcctac attccatact gtttctaccc gtattgacga
     4861 attatatacg actcacgcgt ggactcaagg tggaaaagta aaagaattac ccggagatat
     4921 catcttttgg gcattgcgag atgtggtaag cgaatctggt tattaccaaa acaatgcaaa
     4981 agaaaccaat gatttttgga aaaaagtcaa ccaagaatta gaagaagcct atcacaaggg
     5041 aaccatcaaa aagaaaaaag agttctatct gatgggatca ggagatggaa agacgatgag
     5101 tgatctgcca atcgtattcg atttccttca atcaggatac gcttatgcat ggcgatataa
     5161 agatttccat caaggaggaa attatagttt cggaacgcaa gaacaagtca aacaggctca
     5221 agaattatta aaccttccgc taatggagaa ttggatcgat agtaatgaac cgagaacacc
     5281 taatttccaa ttaacaaaat cagcaaaagt cgcgaatgtc atcataaaaa tttaccaagt
     5341 tctggctatt cccgtgctga ttgcttcaat agcagggata ttacttatga tactaggaac
     5401 cgtatttgca aaagctgatc gtgcctatta tagttcctct ttgattgtgg tcaccggatt
     5461 aatactgaca caattggttt ttcttttcgg tgtctcatgg ttttgttctt atgcgccaga
     5521 aaaaaaggac tattttttaa gtgtctacac tggtgcaggc gtgccattaa cgcaattcat
     5581 cgtagtaaca agcatgctag ttttcagtaa attaaatctg aaaaaagctt tatcttctaa
     5641 ataaaaagac ccttggtagc tagattttta acctaactat caagggtctt tgtgtggaaa
     5701 aatcagtttt tattcgattt cttctaccgt ttcctgcaaa agctgttcat attttttgtt
     5761 tcctttatat aaatcaccaa tcgtccatgc caacaggaga aggacaagaa caatgattac
     5821 ataagcgatg tgatcagcca atgctagttg agcggaagtg gcttgctcgc cgaagaaact
     5881 ttcgatctgt cctggtaatc ctcgtaagtt caagtaagtt agactaatca cagagaacca
     5941 gccaagaagt tttacgatga atgaattttt aaatcggcta cccatctccg cttggctgtt
     6001 ggtcatcatc agtaaaggaa tcatcgaaaa tggcaacgca aaagccaaga acacctggga
     6061 ttcattcatt aagttattca aagcggcatg ttcttcgatc gttccttttg tacttgtaaa
     6121 caaaacacaa atcaatacag gaatcacaga aagcaatcgt gtgaccagtc ttcttagcca
     6181 aatcgggata cgcatatgga tgaatccttc catgatgact tgaccggtca acgtaccagt
     6241 aatagtagag ttctggccag aagcaagaag tgctacagca aataaagtgg acaagatacc
     6301 agattttgct acagcaatca aaataccatt gctcatagct gatggatcag ataaagcttc
     6361 atacagacca aaaaaggaag gatcttggac agaaccggtt ttgaagacag cgacacccat
     6421 gatcaatagc agtgcattga ctagaaaagc cattgacaac tggatattag aatcccaagt
     6481 agaaaaacgt acagcttgtg ctacatcttc ttcgttttgg tgatcgatct tccgagtttg
     6541 tgaaactgct gaatgaagat ataagttatg cggcatgact gttgcaccaa taatccctaa
     6601 agctccagtc agcggagttt cgccgccgat gaccggtgag tctgagaatg tatgactgtt
     6661 agggataaag ccagcaaata aactgtgcca atctgggtta gacaagccaa cttgatagac
     6721 aaaaacgaat aaaatgacga aaattaagca gacaaccagt gcttcgatct tacgaaaccc
     6781 gacttttgtc agtagtaaga gaacgaaaac atctaataca gtaataaaga cggcaatcac
     6841 caatggaata tcaaaaagta agtagagggc gattgctgca ccaatgactt ctgcgatgtc
     6901 tgttgccata atggctaatt cagtcaagat ccataaaatg acacccaatg tcttacttgt
     6961 ccgtgctcgg attgcttggg ccaaatccat ttgggtcacg attcctaatt ttgctgccat
     7021 atactgtaaa agcatagcaa ttagactaga catcaaaata actgacatca ataaatattg
     7081 gaaattttgt ccaccagtga tcgaagtgga ccagttacct ggatccatat aaccaactgc
     7141 tactaatgca cctggtccag agtaagcaag aagtgtttta aaaaaacttt ttccctttgg
     7201 tacatccact gttccattta cttcagctaa agataaacca tttgtatgtt caataagtcg
     7261 atgcttatga aattgtttgt tggattgatt attcaagaaa aaaccacctt tcctatttat
     7321 ctacgattac attctacacc tcagaaagaa aaaattaaag aaaaagattc ggtatgccta
     7381 attttttttc gtggtgtgtc ttgctggtta atgaaagaaa ttttgttctt ctctaaaaaa
     7441 accactattt tttgcatgaa actaaagaat acgcctttct tttttcaata aactttagta
     7501 aaataaatca ttgagagcaa ttcggaaata gaaaggagag atttatatgg tatcaacagg
     7561 tattgtcgta cttattgcta ttattgcttt gctaatcggc gcagtaggtg gctttttcct
     7621 agcgcgtaaa tatatgaaag actatttaga aaaaaatcct cccgttaatg aggacatgtt
     7681 gcgatcaatg atgatgtcaa tggggcaaaa accatctgag aagaaaattc gccagatgat
     7741 gcagcaaatg aagaaccaag ggaaaaaata ggttctgacc caaagacttt ttgcgggctg
     7801 attttttcag tccgtttttt atttcgtaat ttgttagact attcagacta ttcagcaaaa
     7861 aagacaagca cagacaatcc tatgctcttg ataaagacaa aggaggagta aaatgtcgat
     7921 ttttagaaaa ttaggttggt tttttcgtca ggaaaagaaa cattatttaa ttgggatttt
     7981 ctccctattc gcagtggcct tggtacagtt agttcctcca aaagtgattg ggatcatcat
     8041 tgatgagatc gccgataaga atttatcaat aaagatcatt tcctggtgga tcttattatt
     8101 gatccttgca gctttcttac aatatctttt tcgttatatc tggcggacga atatttgggg
     8161 aagtgcagct cggttggaga gagaacttcg gcagcagcta ttcaaacatt ttacagaaat
     8221 ggacaatctc ttttaccaaa aatatcggac tggagatttg atggctcatg caacgaatga
     8281 tttgaatgca atccagaatg tagcaggagc tggaatcttg acctttgcag attcagtgat
     8341 tacgggcgga acaacgatca ttgcgatgat tttgtttatt gattggcgct taacactgat
     8401 tgcgctgatt cctctgcctt tgctagccgt tacttcacga gtgctggggt caaaactcca
     8461 cgatgctttt cgtgattcgc aagaagcttt ttctagtatc aatgataaga cacaagaaag
     8521 tatcactggg atcaaagtca ttaaaacatt tggacaagaa aaagaagatc tcgcggattt
     8581 tgaagaaaag attgaagatg caattattaa aaacaaacga gtcaattttt tagactcctt
     8641 atttgatcca ttcattacct tgattatcgg tctgtcttat gttgcaacaa ttattttagg
     8701 tggaaagtat gtcttggaag gcacgatctc aatcgggcag atggtctcat ttattagcta
     8761 tatcggtatg cttgtttggc cgatgtttgc gattggtcga ttgttcaacg tattggaaag
     8821 aggaaatgct tcttatgatc gcgtcaatga acttttgcat gaaaagacgc atatcgtgga
     8881 acaactgggt gcagtgcaga ccccggcaaa aggcgagctg aatgtagcta tttcctcttt
     8941 taactatccg aaagaagaat caccagcatt agaacaaatc aaatttgcga tccaagaagg
     9001 agaaacatta ggtattgttg ggaaaactgg tgcgggaaaa acgacaatca tcaaattact
     9061 gatgcgtgaa tacgatgatt atcaagggaa aatccaattt ggcggacgtt cgatcaaaga
     9121 atattcattg aatgctttat taggatcaat cggttatgtg cctcaggatc acttcctgtt
     9181 ttcgatgacc attcgagata atatccgttt ttctaatcca aaactttcag aagaagaagt
     9241 agagcatgcg gcttctttag catttatcaa caatgaaatc aaggaattgc caaaaggtta
     9301 tgatacctta gttggagaac gtggagtctc tttatctggc ggacagaaac agaggatttc
     9361 gattgctcga gcattgatca aaaatcctga attgctgatc ttagatgatg cactttctgc
     9421 agtagatgcc aaaacagaag aagctatctt gcataattta aaagaaataa gaaaagagaa
     9481 aacaaccatc attgcagctc accgtttaag cagcgtgatg catgcaaaag aaatcattgt
     9541 tgtcgatcaa gggaaaatca ttgaacgagg aacccaccac gagctgcttg cattaggtgg
     9601 ctggtatgaa cgaatgtggg aaaaacagca attagaagcg aagatagaag gaggcgaggc
     9661 ctgatggatg aaaattatca atcggaatgg acaaaatcga ttccacttaa agaacagaag
     9721 aagatcatca aacgactgat gaagttcgcc aaacctttta gaagaacgtt catcgttgct
     9781 atcttatttg cgtttgccct gtctgtgatc aatgtcctat tgccaaggat catccaaact
     9841 tttatggatg atcatctggc taaacagtca gctacgacac aagttatttt gttttttgct
     9901 ggtgtgtact tgtttggagt catagtcaaa agtatcattt ggtttttcca atggtatttg
     9961 tattccatgg cttcattgaa gacctatcag tatgttcggg taagattatt tgaaaagctg
    10021 catacactag gtatgcgtta ttttgaccag acaccggcag gatcgaccgt ttctcgagtg
    10081 acgaatgaca cagaaacgct atttgaattt tggtatgtct ttttgatggt attgactggg
    10141 atctttgcag tcgtttcttc tttcgttgcc atgtttcaaa tcaatggaga aattgccttg
    10201 tggactttgg tgttcttgcc gattttagga atcgtcatct ggtattacca gaaattcagt
    10261 tctcgtattt accgaaatat gcgggaaaaa ttaagtcagc tgaacacgaa attgaatgaa
    10321 tcgatttccg gcatgcgtat tatccagcag tttcgccaag aagctcgttt agctaaagaa
    10381 tttgaacaag tgaacaacga gtatttagac acgcgctatg cgatgatcaa aacgaattcg
    10441 attctgttaa gtccaatcat caattttctc tatgcattgg cgattgcgtt ggcgttgact
    10501 ttgtttggga tcgatgcgtt gcattctccc gtagaagttg gactgatcta tgcatttacg
    10561 acctatgtcc aggaattttt taatccaatg tctcaaatga tggacttttt gagcaccttt
    10621 acagatggga tggttgccgg tagtcgtatt ttgaaaatca tggatacaga agaatatgca
    10681 ccgcaacaaa atcctggagc agatcaagcg attacttcag gtaagatcga gtttcgcaat
    10741 gtaagctttt cttatgatgg agagcacaat gtattgaaca atatctcttt tgtcgctaat
    10801 ccgggtgaga ccgttgcatt agtggggcac accggaagcg gaaaaagctc gatcatcaac
    10861 gtattgatgc ggttttacga attttatgaa ggccaaatct tgattgatga tcatgacatt
    10921 cgagaatatc caatggaaga gttacgtaag aaaatgggat tggtcttgca agatgccttc
    10981 ttgttttatg gagatattgc aggaaatatc cggatgatga atccagctat cacagacgaa
    11041 cagattcgcc atgcagctga gtttgttcaa gcaaacaagt tcatcgatga attaccaaaa
    11101 ggatatcact caaaagtgat cgaacacggt gccagctatt ctagtggaca acgacagctg
    11161 atcacttttg cgcgaacaat cgtgacagat cctaaaatac tagtattgga tgaagcaaca
    11221 gcaaatatcg atactgaaac cgaaggattg atccaagaag gattggcaaa aatgcgacaa
    11281 ggacggacaa cgatagcgat tgcccatcgg ctgtccacga ttcgggatgc agaattgatt
    11341 ttagttttag acaaaggtca tatagtagag cgtgggaatc atgaaacact tctagcgcaa
    11401 aatggtttgt atgcagatat gtacaaactg caaagcagtg gtgtgtaaaa agaggttgtg
    11461 acagtagcat agtagcattt tgccctaagc gctagagtga aaataaaaca gagcttcgca
    11521 gattcttcat ctgtgagcgt tagtggcgta gtcacccgat ttaaaaaaga cgacgacagc
    11581 tagtcaaaag cggctaataa ccactaagaa agcgttgaca aaagcgtttg ctttcgataa
    11641 tataaagaaa acgatacaac ttggatagtg attgcctgat gcaagctaaa cctgatgagt
    11701 ctcccatggt tccatggacg gctttttggg tttttctttt tgttttggat acagccctat
    11761 atatcatgga aaggagcaaa acatgctgat tgagaaagta ctgaataaca acgtcgtaat
    11821 aaccagggat gaaagaaatc aggaaatgat cgtgatggga aaaggtttgg cattcaaaaa
    11881 gaaagtgggt gacccgattt ttgactcatg catcgacaaa aaatttcagt tgacaaatga
    11941 cgggctttca caacagttcc aagaattggc acaagcgatt cctcttgagt acttagaaat
    12001 ttcttgtgaa atcattgaat atacaaaaga ggctctacaa gtccagttga atgattctgt
    12061 gtatattgca ttgacggatc atttgtatac ggctatcgaa cgtcacaaaa tgggaatatt
    12121 tgtaccaaat gtattgctgt atgatatcaa acaactattt cctaaagagt atgccatcgg
    12181 gaaaaaaaac agtgaagaaa atctcggata aataccacat caagttatca gaagatgaag
    12241 ccggatttat cgcattgcat ttagtcaatg cacaaggcat tcaaagccaa caagacaata
    12301 cctacctgtt cactataaca atacaggata ttctaaatat cgtccgctat tactataaga
    12361 ttacatttga tgaagattca gtccattttt ttcgattcgt cacacactta cgtttttttc
    12421 tgacacgcgt cctagatgga gaaaaatcag aagtgggaga agtttctgag gaattgtttc
    12481 aggttattca aaaaaaatat aaggatacgt ttggttgcgt tgagaaaata gcagactttt
    12541 tagccgagac gttcaactac cagatgtcca cggacgaaaa aatgtattta acgatccata
    12601 ctgcgcgttt agtgcatgag aatcgttgta aataaataaa aaaaggagga taaattaaca
    12661 agaacgtatg aaataagggt agtgacattc agttggctaa accttccact aataaattgg
    12721 agggaataga aatgggaaaa taccaagaat tagcgagaaa aatcgtagaa aatgtcggcg
    12781 ggaaggaaaa tatcaatggt ttgacacatt gtatcacaag gcttcgtttc aaattaaaaa
    12841 acgaagaaaa agcaaatgat gagatcttga aaaatatgga cggtattgta acggtaatgc
    12901 gagcgggagg gcagtatcaa gttgtcatcg ggaatcatgt gccagtcgtg tttgaagagg
    12961 ttataaaagc aggaaacctt acttttgatg aggctgtttc aacagaaaaa atgagaccgt
    13021 ttgatatgct tattgatgga atatcgggct gtttccaacc attccttgct atcctagctg
    13081 ctgacgggat gattcgtgga ctgacagcat tccttgtctt cttaggcgcg tttgaccgcg
    13141 gttctggcac atttgttatg tttgataata tcggtgattc tgtattccaa tttatgcctg
    13201 ttattatcgg tttaacagct gcccgtaaat ttaaagtaaa tgaattcgta gggatgttgc
    13261 ttggcgcagc attaatgaat cctagcttgt cgctggaagc tttgagtggt gcagcagaag
    13321 caccactaac gaccatattt tctggcacta tttttgaagc acctatttat caaacagtat
    13381 tcggcattcc ctggattgcg cgtaactacg cttcttctgt cattcccatt atctttattg
    13441 tgttgttagc tagccaaatt cagaaaccaa ttaaaaagtt agtacctgag atgatcgcga
    13501 atttcttcgt tccattcttc accgttctta ttacaatgcc tttaggtttt ttactcgttg
    13561 gaccagtatt cacattcgca actgatattt taatggctgg atttgaaaca cttttagcac
    13621 tttcgccagt tatttatgga gcaatcgtag gcttcttctg gcaaattctg gtgatgtttg
    13681 gtctccattg ggcaatcgta ccaatgggat tgatgcaatt ttctgtgaat ggttggcaaa
    13741 atattatgac acctgttgca gtagtgtctt ttggacaaac tgcagcactt accgcactct
    13801 actttaagct tagaaatcca aaagacaaag caattgcaat accagccatt gtttctggta
    13861 ttgtggggat tacagagcca gctatttatg ggtttacatt gccacgtaaa aaaatattta
    13921 tttttacctg tgtcggtggt gcgttagggg gcgcgtatag tgggttaatg aatttaactt
    13981 cttggaatca aggtggacta ggcatcttta caattcccaa ctatattaga ccagatggtg
    14041 atttaacaga tgtgattaat gtattgattg ggatcgccat tgcgatggtg gtatcctttg
    14101 cgctgacttt tttcttttgg aaggaggaag cagatgaaac agatgataga cagaaaagat
    14161 caggtaaaga aattgtaaaa acaccgatac aaggacaaat cgctccactg aatgcagcaa
    14221 aggatgcagc ttttgcacaa ggaacactag gtcgaggaat cttgatctac cctgaaaaag
    14281 gagaagtacg tgcccctttt gacggaacag tcatgtccct gtttccgacg aaacatgcaa
    14341 tcgggatggt ttctgaagca ggtctggaat tgctgattca tgtcggattg gatactgttc
    14401 agttggaagg gaaatatttt gaaagcttgg tgcagcaagg agaaagagtg aaacaaggcg
    14461 atctgttgct tcgcttcgac aaaaagaaaa tcgaagagga aggatacagt ttagaaacac
    14521 ctgtgattgt catcaattac atggattatt tggatatttt ggaaaatcaa ggggaaacgg
    14581 tcgggccaga cgatgcactt atcaccgcac tgacctgaac ggttaggtga aagaatggac
    14641 taggcttaac tattcttttt agtccacttc tttattttca aaattgaaag gctctttcat
    14701 gatggctgag ctgatagggc gtttatcaaa tttttgtcct acagaaatta gatggactaa
    14761 attctgttga taattttctg caaaaacaca actatataac acgtctagaa gataacaaat
    14821 cgatgtattg atcgtaaaat taccaatttt cgaatacagt cgttcgcgtg tcgtaagtcg
    14881 caaagcggct gtactcattg aagatagcgt tgaatcaccg atactggtca aagctaaaat
    14941 tggcacatga ttttgtttca atatttctgc ggtacgaagg atcacattgt tttcacccgt
    15001 ataagaaatc agaatcccac acatatcact tgtgcaattg tacgcttcat agaacgattc
    15061 accaaaagtg gtgacaatcg tgacatccat tttgatgcgc ttcattttca gcatgaaatc
    15121 ttgagaaatc aaagtattcg agttactggc gaaaatcacg attttttttg cttgaagcat
    15181 tagttgtttg gctttttgca gttggtcatg atgaatgaga gaaagtgtat cttgaatcgt
    15241 catctgttct aaagaagcca ttttatttgc gatcgtcata ataccgtctg tggtggaaaa
    15301 aggaagattc gcatctacgt cttgaaaatt cccttcaaga taggctgttt cagctaaaaa
    15361 agcattacga aaactgaccc agccatcaaa acccattttt tttgctacac ggatgagtgt
    15421 ggacgggtgg acataacatc gctcagcaat ctcttggatg gtcatttcgc caagtgctgg
    15481 agaatcgctt aagagaaact gaatcacttc attttctgaa ggagaaaagg agatttcttt
    15541 gattttttca cgtaataaca tgtagatacc ttcttcttgt ttttttacgt aaatcataac
    15601 ttttttgaaa atgatttgta aacggattta tgttatatag cttaataaag acattttttg
    15661 acaagatatt tacaaaaatc cagaagaaaa cactaacttt tgtaaatgtt actggaaaga
    15721 aacgaatttc tattgtttgt tttgtttaca aaaaagataa taaaagtgta aaaagtaaag
    15781 gagtgagaac atggcaaaag gaatcaaaat cgctacgatt ggtggaggca gcagttacac
    15841 ccccgagttg atggaaggat ttatcaaaag atatgaggaa ttaccgatac gagaaatctg
    15901 gctcgtagat gtagaagaag gaaaagaaaa gcttgagatc gtcagtcaga tgtctcagcg
    15961 tatgtgggat gcttctccgt atgaagtcaa aatctatgca actctggatc gggaagaagc
    16021 attgaaagat gcggattttg ttacgactca gtttcgtgtc ggtcaattag atgcacgaat
    16081 caaagacgag cggatcccta gttattatgg gatgattggg caagaaacaa atggtgctgg
    16141 cgggatattc aaagcttttc gtaccgttcc agtggttttg gcaatcgttg aagacatgaa
    16201 gcgtttatgt ccaaatgctt ggctgattaa tttcgcgaac ccaagcggga tggtgacaga
    16261 agcagttatc agatatggca aatgggataa agtgatcgga ttatgcaatg tgccagtaat
    16321 ggcgatgatg acagaaccaa caatgcttgg caagaaaaaa gaagagctga tctacaagtt
    16381 tgccggtcta aatcacttcc attggcacaa agtagcagat aataaaggaa atgaccgcac
    16441 ggcagaattg attgatcgtt tatacgatga agacaatgga ttgccgaaaa atatctttga
    16501 catcccgttt ttcaaagaac aattaaaaca aatgaacatg atcccttgtg gctaccaccg
    16561 ctattactat cgtgaagaag aaatgctgga acatgcttta gaagaatatc aaacgattgg
    16621 tacaagagcg cagcaagtga aagaaacaga agcagagctg tttgaattat acaaagatcc
    16681 agaattggat cataaacctg aacaattgca aaaacgaggt ggtgcttatt attcagatgc
    16741 tgcctgtgaa acgattgctt cgatctatgg aaataaagaa acacagatcg tggtatctac
    16801 aaagaatgat ggagctgttc ctgatctgcc ggcagattgt gtagtagaag tcactgccta
    16861 tgttggcgca caaggcgcac gaaatgtagc gtttggttcc cttcctgctg ctgaaagagg
    16921 ctggttgcaa gtaatgaaga atatggaatt gctgacgatc gaagcagccg tgactggtga
    16981 ttatggaaca gctttgcaag cgtttacgat caatccgctt atacgatcag gaaagacagc
    17041 aaaacggatc atggatgagt tgtttattgc acatcagcag tatttaccgc aattcaagga
    17101 aacaatcagc cggttaaaag cagaaggaat cgaagtccaa gatgaactgg caagagaatt
    17161 atcaagcaat acacatgtaa tggaggaagt atgatgggat tcagaaaaga ttttttatgg
    17221 ggcggagcta cagcagccaa tcaatgcgag ggcggttatg atgaaggcgg acgtgggtta
    17281 gcgaacgtag atgttgtgcc aatcggagaa gaccgttttc ctgtcattac tggaaaaaag
    17341 aaaatgtttg aatttgatga aaatcatttt tatccagcaa aaattgcgat cgatatgtat
    17401 catcattata aggaagatat tgctttattt gcagaaatgg ggttcaagac ttatcggatg
    17461 tccattgctt ggagtcgtat ttttccaatg ggcgatgaaa aagagccaaa tgaatctggg
    17521 ttgaaatttt atgaggatgt ctttaaagaa tgtcaaaagt acgggatcga gccgcttgtg
    17581 acgattacac attttgattg tccgatgcat ttgatcaaag aatatggtgc gtggcgttcc
    17641 agagaactcg ttggttttta tgaaaatctg tgccgagtca tcttcaatcg ctataaagga
    17701 cttgtcaaat attggttgac attcaatgag atcaatatga tccttcatgc accattcatg
    17761 ggcgcagggc tgtattttga agaaggagaa aatgaagaac aaatcaaata tcaagcggca
    17821 catcatgagt tggttgcaag tgccatcgca acgaagatcg ctcatgaagt agatccggag
    17881 aacaaagtag gctgtatgct ggcagcagga gcatattatc catacacttg caatcctgaa
    17941 gatgtttggg cttctagaaa gtcagatcga gaaaattatt tcttcattga tgtccagtca
    18001 cgaggagaat atcccgctta tgcattgaaa gaaatggaac gaaatggcat tcagatcaaa
    18061 atggaacctg gggatgccgc attgctgaaa gaacatactg ttgattttat ctccttttct
    18121 tattattctt ctcgagtttc gactactgac aacgaactct tggaacagac tgcaggaaat
    18181 atctttgctt ctgtcaaaaa tccatatctg gaagcaagtg aatggggttg gcaaattgat
    18241 ccattaggct tgagaataac gatgaatgat atttatgatc gttatcaaaa accactattt
    18301 attgtagaaa acggcttagg tgcagtcgat acgcccgatg agaatggtta tgtggaagat
    18361 gattatcgaa tcgactatct ggcagcacat atccaggcaa tgaaagatgc agtagaatta
    18421 gatggagtcg atctattagg ctatacgact tggggatgta ttgatttagt atcagctggt
    18481 acaggtgaga tgaagaaaag gtacggattc atttatgtcg atcgtgacaa cgaaggaaat
    18541 ggcacattag cccgttcgaa gaagaaatca ttcaactggt ataaaaaagt aattgattca
    18601 aatggagaaa atctttcgaa cgattgatca atgatttata aaagtctatt gtttttcagc
    18661 ggatttttcg agtataataa agctattcga gaaacgcaag cagaacaata gacttttttt
    18721 cgcattggct gtaaagtatg cttacttgat taagtaaagg agaatctaca tatgtatgat
    18781 gtaatcgtta ttggcgcagg gcctgctggg atgactgctg ctttatatgc ttcacgttca
    18841 aacttatccg tattgatgat cgaacaaggc gctcctggcg gccaaatgaa caatacggca
    18901 gaaatcgaaa actatcctgg tttcgattca atcatgggtc ctgatctagc ttataagatg
    18961 tatgaaggtg tttcaagatt tgggacagaa aatgcttatg gaatcgtgca agatatcaag
    19021 gatcacggca gccacaaaga agttatttgt gaagataaaa cttacgaagg aaaaacagtc
    19081 attatcgcaa caggctgtat ccaccgtaaa ttaggcgtac ctggcgagga agaatttgct
    19141 ggacgtggcg tttcttactg tgcagtttgt gatggtgcat ttttccgtaa caaacgtcta
    19201 gtagtgatcg gaggaggaga ctcagcagta gaagaagcca tctatttgac acaatttgct
    19261 tctgaagtag tcatcgtcca ccgtagagat gaattacgtg cacaaaagat catccaagac
    19321 cgtgcatttg cgaatgaaaa aatttccttt atgtgggata cagtcgttga cgaaatcgtt
    19381 ggaaatgaaa tggtagtgac aggtgtgaaa gcacgtaatg tcaaaactgg caatgagttt
    19441 gatttggcag cagacggagt attcatctat gtaggattgg atccattaac tgcaccattt
    19501 agagaagctg ggttgacgaa cgaagccggc tggattccaa cagacaacga aatgaagacg
    19561 aatataccag gcgtttttgc catcggagat gttcgtgaaa aagatttgcg acaaatcact
    19621 actgctgttg gtgatggtgg gattgctgga caacaagtgt ataaatactt ggaatcttta
    19681 gaagaaacaa ctgcataagt ttaaaaagag tgaaagaacg gtttcaattg ttcttttact
    19741 cttttttttg cgtgaaatct actgaatccg atttatgcga atacttgtaa ccgtgcttga
    19801 taaaagaatc ttttcttgat ttaagcgttt tttggtcctc atactttcat atctaataag
    19861 tgaatgctat acttattaag aaccatattt cagaaagagg tgttattttc atgtcttggg
    19921 aacaagtgta tgaacaatgg gtacatgaag aaaatttaga agaaaactta aaaaaacaat
    19981 taaatgatct gggtgaagat cctgaaaaac ttgaagatgc tttttatgca ccgttggaat
    20041 tcggtactgc aggcatgcgg ggaatcttag gacctgggat caatcgaatg aacatctata
    20101 cggtaagaca agcaacagaa ggacttgcac gtttcatgga tacacaaggt gaagaaacaa
    20161 aacgccgagg tgtcgctatc gcttatgact cacgccatca atcaccagaa tttgccatgg
    20221 aagcagccaa aacattagca catcacaata tcccttctta tgtattcgaa agtttacgtc
    20281 caacaccaga gctttctttt gcagtccgtc atttgaagac gtttgcggga atcatggtaa
    20341 ctgcatccca caatccagca gcttacaacg gatataaagt ctacggagaa gatgggggac
    20401 aaatgcctcc tgcagacgca gatgcattga cgaaatatgt ccgtgaagtc agcaatccgt
    20461 taaaagttga agtattatct gacgacgaag ctgaacatag cggattgatc acgatcatcg
    20521 gagaagaagt ggatgccgct tatctagaag aaatcaaagc agtaacgatt gatcatgaat
    20581 tggtggaaac aatgggtaaa gatttgaagc ttgtgtacac accacttcac ggaacaggaa
    20641 aaatgttggg cgaacgtgcg ttgaaacaag ctggattcga acaattcgta ctagttccag
    20701 aacaagctgt cgcagacccg gatttcacta cagtcaaatc accgaatcca gaagaacact
    20761 ctgctttcga gtatgcgatg cgacttggcg agaaagaagg cgctgactta ctgattgcaa
    20821 cggatcctga tgcagaccgt ttaggcgcag ccgttcgttt gccaaatggc tcctatgaag
    20881 tattgacagg aaaccaaatc ggagccctgt ttgctcaata tattttggaa gcacaccaat
    20941 cgcgaggcac tttacctgaa aatgcagcgg tattgaaatc aatcgtttct agcgaattgc
    21001 ctacagcaat tgcggaacac tatggggcta ccatgttcaa tgtattgaca ggattcaaat
    21061 tcattgctga aaaaattcaa caatttgaag aagatcattc ccatactttt atgttcggat
    21121 tcgaagaaag ttacggttat ttagttaaac ctttcgttcg cgacaaagac gcgattcaag
    21181 cattgctctt attagcagaa gtagcagctt actataaaaa acaaggcaaa acattgtatg
    21241 acggcttgca aacgattttt gaaacatatg gctactacgc agaaaaaacg atttctgtga
    21301 caatgagcgg acaagaaggc tctgcaaaaa tctctgcttt gatggaaaaa ttccgtgaag
    21361 aagcaccgac agaatttgcc ggagcaaaag tcatccaaac agaagacttc aaagagttga
    21421 cgagaacaga ccgtgaagga aacgtagaaa aactaacgac accgccatct gacgtattga
    21481 agtatacgct ggaagaaaat ggctggatcg ctatccgtcc ttcaggaact gagccgaaaa
    21541 tcaaatttta tatcggtgta aaaggaaact cttcagaaga cgcagctgaa aaagtagccg
    21601 cttatgaagc gattatcaaa aaaatgacag aagaataaaa aaacttgaga ctgatggaaa
    21661 aagtggtaaa ctttagaagt cagtcttttt tcaagttgga ggtgaatgga ttgccagaca
    21721 cgcaaaaaac aaaagtagag aaagtcagcc aactattcaa aatgttgagt gaccccactc
    21781 gtctcaaaat tctgctgtat ctgaaagacg gggaacaaaa tgtgacagca atcacccaag
    21841 cagtagaaat ggaacaatct gctgtatcgc atcaactgcg tttattaaga gaaaaccatg
    21901 tcgtcaagtc ccatcgtgag ggaaaagcga ttttgtatag tcttgatgat tctcacgtac
    21961 tggatatttt gaaccagacg ctcaaacatg tggaacatca ataacgatag gtgcaaatag
    22021 agacaaactg gactgtatca acgaatcttc ttcgctgaca cagtccagtt tttttaagaa
    22081 aaagacaaga aaaactgtta gtctgtttct tcggtggttt cagttccata ataactatga
    22141 tcttctaggt ctaaagcatt caaaataagt gaagctgttt cttcgataga taaagaagcg
    22201 acattgatta ctacgcagcc caatttttca taaagatcat tcgcaaaatc gagttcagct
    22261 cgtatttttt cgatatcgga ataagcagta tcaggattta atccgtaaga gcgcatccgt
    22321 tctttacgga tattgtttaa aatatctggg ttattcgtca gtcccacgat ttttttagga
    22381 tcgatctccc aaagctgttt gggaatatgt gcttgtggga tcaaaggtag attggcaacc
    22441 tttaaatttt tgttagctaa aaacaagctc aaaggcgttt tggaagttct ggatacgcct
    22501 agcaaaacga catctgcttc tagaaaccca cgaggatctt tcccgtcatc gtattttaca
    22561 gcaaattcca ttgctttgat ccgtttgaaa tagttctcat tcaataaatg aagggcgcca
    22621 ggttgccgaa tgggcttgat accagttaag cgctcgattt ctgatatcgg cggagtcatc
    22681 acatcaaaac taaataattg atgttcttca caaaaagaac gcgtcatctc gatcaattct
    22741 tgattgatca atgtgtgtag gacaagggct ttttctttta gtgcatcttg cagtgcctgc
    22801 atcaatgttt tctcatctgt cacaaatgtt ctgcggaata aattgaactc aacggtagga
    22861 tattgggcca tagtggcttg cgctaatttt gtcgcagttt ctccagcaga gtcagagatg
    22921 acaaaaaaag taatgatttc tgtagaatcg gaagacatgg ataaaacctc acttgcattt
    22981 tttttatgtt tattatacca tactctagaa tgtgtacttc gtaatcattt tgatttggag
    23041 gaataagtca tgaaaaaaca gacattgatt gaacaatcac tagaaacatt gaaaaataac
    23101 ggttttaaat atactaaaaa aagagaagca ttgttaactt atttagtgaa gcgcaatcgc
    23161 tacgtatccg ctaaagaagt ttttgactat atgaatcagg aattcaaagg agtaagttac
    23221 gatacgattt atcgaaatct ccatgatttt gcagaaatcg atttactgga agaaaccgat
    23281 ttaaatggag agatgaagtt tcgttttcat tgctgtcaag gggaaatcgg ccatcaccat
    23341 cactttatct gtacggtgtg cggaacaacg aaagaaatcc atatgtgtcc aatgaattat
    23401 ttcgaagaac agctcggtgg atgtacgatc gaagggcata gattcgagat tttcggaaga
    23461 tgtgaaaatt gtcaataagg atttaaataa aataacattt gataatttta aaaaaaagca
    23521 agatttcctt gacggtcaaa acagcattag atataatatt tacaggacag tcttgttttt
    23581 tgagatatat ttcaataaca attacggtta ctttttaagc tcggagggag ggaattaaca
    23641 tgtcaaaaac agtcgttcgc aaaaacgaat cacttgacga tgctcttcgt cgcttcaaac
    23701 gttccgtgtc aaaagcgggt actttacaag aatctcgtaa acgtgaattc tacgaaaaac
    23761 caagtgtaag acgcaagaaa aaatctgaag cagctagaaa acgcaaaaaa ttctagttct
    23821 gtgatgaatt ggagttggta gtgtgtcact actaagtaga ttaaacgatg acatgaaaac
    23881 agcgatgaaa gcgaaagata aggaatcttt acaagtcatc cgaatgatca agtcatctat
    23941 acaaaacgag cagattaaag aaggtcacga cttgacagaa gaagaagaat taacggtgtt
    24001 gtctcgcgaa atgaaacagc gacgtgattc tttgcatgag tttgaagaag caggacgtga
    24061 cgatttagct gaaaaagtaa aaagtgaaat tgtgatagtc gaaaaataca tgccggaaca
    24121 actttccgac gaagaaattc gacaacttgt tcaagaagca atcacacaaa ctggtgcttc
    24181 atcaatgaaa gaatttggca aagtcatggg tgcgatcatg cctaaagtca aaggaaaagc
    24241 agatggaaac caagtaaatg ccattgtgaa agaactatta caatcttaat tttatcattt
    24301 aaaggctctt cgtcaatgag tgttggtgaa gaaatt
//
LOCUS       DACGLE010000037        23827 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0534
            SAMEA3304449-rid9560453.denovo.038, whole genome shotgun sequence.
ACCESSION   DACGLE010000037 DACGLE010000000
VERSION     DACGLE010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304449
            Sequence Read Archive: ERR985064
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23827)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23827)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702225.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702225.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 20:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,045
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 27 of 67
            Pseudo Genes (incomplete)         :: 25 of 67
            Pseudo Genes (internal stop)      :: 19 of 67
            Pseudo Genes (multiple problems)  :: 4 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23827
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304449-rid9560453.denovo.038"
                     /strain="VREN0534"
                     /db_xref="taxon:1352"
     gene            complement(53..787)
                     /locus_tag="IXT20_001604"
     CDS             complement(53..787)
                     /locus_tag="IXT20_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HAQ4860051.1"
                     /translation="MDYSLMTRLAAEMIGTALLIVLGNGAVANVDLKGTKGYGSDWML
                     IAVGYGFGVMMPAMIFGGISGNHINPAFTIALASNGLFPWAEVVPYIIAQLIGAAIGQ
                     LIVVACYKPYYDQTEDVNHVLGTFSTINSVGSKFNGFVNEFFGSFVLFFGALGITKAP
                     FFQDNLGTAHLALGFLVWTLVASLGGPTGPGLNPARDFAPRLVHALLPLKHKGSSQWG
                     YAWVPVVAPICAGVVAVFLYKLLFMA"
     gene            1114..1524
                     /locus_tag="IXT20_001605"
     CDS             1114..1524
                     /locus_tag="IXT20_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HAQ4860052.1"
                     /translation="MKFFQEFFIIIFFSVLGEGIRIFAHLPIPGSILGILFLFLAFEM
                     HLVDPEKIGATGDFLLNNLAILFVPAGVGLLEYFDDIASIWPVLLGAVVVCSIVTMVA
                     AGKTAEGVEALLGYVRKKKAERVSVNEMEENQID"
     gene            1517..2218
                     /locus_tag="IXT20_001606"
     CDS             1517..2218
                     /locus_tag="IXT20_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HAQ4860053.1"
                     /translation="MTEFFVNPLFGLSLTLGVYVLSDKFLRKYKLPVLNPLVLSIVVI
                     ILFLHFSGISYKDYNIGGNFLTMLITPATVALAIPLYKNFRLLKENFYPVMAAILAGI
                     VANCLVSVGFGYFFSLHKEMVISLLPKSVTTAISVDLSHTMGGVNAVTLAIVVSTGIF
                     GSLIATHIFRLFKIESPVARGVALGSTSHAIGTAKAIEIGEIEGIISGLAICVNGILT
                     VLLLPLFFQPFAGLF"
     gene            2218..3804
                     /locus_tag="IXT20_001607"
     CDS             2218..3804
                     /locus_tag="IXT20_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HAQ4860054.1"
                     /translation="MRKPLFYATITKTFEHKKGGKDVYLSSEYLKQFDKDLYYKILLL
                     ESFEDQAWHTAAQLAQVVQLDARSVSKYLNELSKNYQQFSGKTHPLFTKNHRSGYNFY
                     DTLDSIEHERFLIYLVQSTLKFQLLHDIFFEEFHTMYQFAQKHYISESTAHRKINEWK
                     QQLQTYGIRLQRGTYIAQGEEEIIRLYLHMTFWQLFRGKIWPFETISQMDVKNMAEHI
                     MAFFNVRLNEIKKRRLEYMLGAFFLRKSQKHYVVLNEKKRRLISDNLLFQRFCQVMEP
                     VFPNYFQVEDELGALFLVLMTREEYYSDPKIRKKIFDFHQQAKTPPFTALSEAKAALS
                     LYQEEQGLPAENLTFEAENYLFSSHFFAYLFPNAKETIDGNSSDFINHLVIENKELKQ
                     WLVHFFESRHKHPNHLAFKNHAFLMGRYLTVFKTLGAFTPQLPKITILLMTDFPLFEE
                     QLLEEGLRTFFRNEYQLIFLPTDYRGREVDLLISTSKVHRKPWADLDYFIVTEELKLI
                     DYIQLSQKFEMIQKQKLLKQ"
     gene            3823..4044
                     /locus_tag="IXT20_001608"
     CDS             3823..4044
                     /locus_tag="IXT20_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2829 domain-containing protein"
                     /protein_id="HAQ4860055.1"
                     /translation="MTFEEILPELKKGAKIIRKGWSGFELYVVLVSDDEFDGCPVTPY
                     LLIKTSDEGFSSFAPTVCDILADDWAIVE"
     gene            4041..4802
                     /locus_tag="IXT20_001609"
     CDS             4041..4802
                     /locus_tag="IXT20_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HAQ4860056.1"
                     /translation="MKNQYPDLAKQVVFITGAASGIGAAQARAFLEQDAFVFGLDQKF
                     GKMEEISLRFPETFAYALGDVRQMKELQQAVSKCQNHFGEVTILLNTAGILDAYKPLM
                     ETDESLWDLIYETNVKSMYQLTKIILPDMIRQKSGTIINMASIAGLIAGGGGIAYTSA
                     KHAIVGFTKQLALDYAGQGIRVKGIAPGAIQTPMNAADFEGKGEMAEWVANETPVKRW
                     AQPEEVAELTLFLASPQASYIQGTIIPIDGGWLLK"
     gene            5182..5691
                     /locus_tag="IXT20_001610"
     CDS             5182..5691
                     /locus_tag="IXT20_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HAQ4860057.1"
                     /translation="MEKNNTRSAKRWTTTDVTRMALVTGLYVAVTLVLSVFSFGVIQI
                     RLSEMFNYLSLYNKRYIVAVTAGVALANIASPLGLIDVAVGSVSTLLVLLINYKITSR
                     IKNMKIKMAVTACVFAFSMFTVAGQLTILYQLPFFLNWWVIALGELASMIVGGIIIYW
                     IGKKVDLSK"
     gene            complement(5766..6305)
                     /locus_tag="IXT20_001611"
     CDS             complement(5766..6305)
                     /locus_tag="IXT20_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HAQ4860058.1"
                     /translation="MNRTLKELILAAEFAAIISVLSQMTIPFGLVPLTGQTFAIGLTA
                     TFLGRKLGLISVGVYLLLGLIGLPVFSGMTGGIAVLFGPTGGYLIGFLLQTWITGWMI
                     ERTDAHYLYAIFANLMGSLAALICGTIWLKISGDLTFTTAFASGLLPFLLPEAIKATG
                     AAYIGILMKKRLNRFLHTN"
     gene            complement(6445..7056)
                     /gene="rpsD"
                     /locus_tag="IXT20_001612"
     CDS             complement(6445..7056)
                     /gene="rpsD"
                     /locus_tag="IXT20_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010766410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HAQ4860059.1"
                     /translation="MSRYTGPSWKISRRLGISLSGTGKELARRPYAPGQHGPNSRGKQ
                     SEYGMQLTEKQKLRHMYGMNERQFRNLFVKASKIKEGKHGVNFMILLEQRLDNVVYRL
                     GLATTRRQARQLVNHGHILVDGKRVDIPSYHVEVGQVISVREKSQNIVTVKEAVEATV
                     GRPAFVSFDAEKLEGSLTRLPERDELYPEIDEALVVEYYNQSL"
     gene            7372..8169
                     /locus_tag="IXT20_001613"
     CDS             7372..8169
                     /locus_tag="IXT20_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate-nitrite transporter"
                     /protein_id="HAQ4860060.1"
                     /translation="MNPVSPLFEKIDASIEKKANLFENSFSRYAVRAVLACLFLTLGT
                     AVAFAIAMKGEDIAHGLGKILYAFMFSWGLVMILYMNAELGTSNMLYMTVGVFRQRLA
                     LSKAAKILFACIFFNMIGGILCGFLISMTTPFQDLTLDNFMLESIAVKLAKPSATILV
                     EGMFANIVVNTAVLISMRMKEDVGKVITVVFIIFIFAFLGYEHVIANFPAFTLTYFAS
                     HGQMDGMTVSNVLHNLFFALAGNYIGGGLVMGLGYAWLNQSKSSYVD"
     gene            8197..8832
                     /locus_tag="IXT20_001614"
     CDS             8197..8832
                     /locus_tag="IXT20_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HAQ4860061.1"
                     /translation="MEVLVFPKNEEKALQMEKYMKNLFSFAGVPKPERALLQQPLLDE
                     SKNLSISEVLAFVHYYYEQIEREYQYVAIDLVQKNIKRLTFEDLVVLLPLVQKKEWWD
                     SIDSWRKVYSTWCKAYPEQREEVFQLFEKKEDFWLRRIAITLQLGYKQQTDTGLLIRA
                     IDEDRQTKEFFIQKAIGWALRDYSKTDADWVKRQLTTDLSKLAVREASKYL"
     gene            8852..9625
                     /locus_tag="IXT20_001615"
     CDS             8852..9625
                     /locus_tag="IXT20_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAQ4860062.1"
                     /translation="MRRKLFAFDIDGTLLDSNKQALDSTREALEKLRKAGHLVTIATG
                     RSRFHAQEIIRDLDFTNYILCNGAAGFLDHEQVYKNLLDRDQLDAFVQEAHHEQIDTA
                     FVSLDNIKRSSSNDVTVMEEAMHSFGAVLPELDQFFAEEQEIYQALAFFDQTYDGKFS
                     SYDKIRFVRWHENSVDVVPKNGSKAATILHLAEQVGIPHEDIISFGDGQNDREMLRMS
                     GIGIAMGNAVPEVQAEAKMVTDTNDQDGIWKALKELSVI"
     gene            10033..10830
                     /locus_tag="IXT20_001616"
     CDS             10033..10830
                     /locus_tag="IXT20_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HAQ4860063.1"
                     /translation="MRVLFIGDVVGSMGREMITEYLPRLKKKYRPQVTIVNGENAASG
                     RGITEKIYKKFLQDGVDVVTMGNHTWDNRGIFEFVNEAKKMVRPANFPEGTPGQGMVF
                     VKVNQVELAVINMQARSFMVDLDDPFRKMNELVEEARKRTPIIFVDFHGETTSEKQAM
                     GWFLDGKVSAVVGTHTHVQTNDARILPRGTAYLTDVGMTGPYDGILGMRREPVIEKFL
                     TALPKRFEVVEQGRSILSGCILELDDTTGHAKEIQLIQINDDRPFME"
     gene            10808..11221
                     /locus_tag="IXT20_001617"
     CDS             10808..11221
                     /locus_tag="IXT20_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4860064.1"
                     /translation="MIDRSWSRKRGSFLQQEPEIQKEVRKLTKLLRENETIIRYKELE
                     EKIQQNQYLAELREKIKQAQKDAVHFAHYDKPAAEKEAIKQADQFMQEFDQHPLVVAY
                     RKQLLEADDLLHHLTTMIQEEINGQIEEEKHASKN"
     gene            11205..13850
                     /gene="mutS"
                     /locus_tag="IXT20_001618"
     CDS             11205..13850
                     /gene="mutS"
                     /locus_tag="IXT20_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HAQ4860065.1"
                     /translation="MPQKTKNTPMMEQYLSIKAQYQDAFLFYRLGDFYELFYEDAIKA
                     SQLLELTLTSRNRNAEEPIPMCGVPHHAAQGYIDALIEKGYKVAICEQVEDPKTTKGM
                     VKREVIQLVTPGTVMDSKGLSAKDNNFLTAVVSQGNEFGFAYADLSTGELKTARLHDE
                     EAVLNEASALQTKELVLGSEITDTLREQLSGRLGLVFSQQNEMEENAEFSFLTSELVD
                     PLEKEATGKLLTYLSVTQKRSLAHIQKAVEYQPDHFLKMDYYSKFNLELSQSIRTGKK
                     HGTLLWLLDETKTAMGGRLLKQWLDRPLIQAKQIKARQEMVASLLDSYFERIDLQSAL
                     TKVYDLERLAGRVAFGNVNGRDLIQLKTSLEQVPMIRGLIEGIDQGQWQSLLSEMQPM
                     DHLVQLIDQAIQDDPPLQITEGNIIKDGFNEQLDMYRSAMRNGKKWLAELEAKERQET
                     GIKNLKIGFNRVFGYYIEITKANLGNAELEKYERKQTLANAERFITPELKELETQILE
                     AEEKSVDLEYQLFLAVREEVKKAIQPLQVLAKAISAVDVLQSFATISERYQYVRPELV
                     SDKHQLLIKDGRHPVVEKVLGHQEYIPNSVEMAEDEMILLITGPNMSGKSTYMRQLAL
                     TVLMAQMGCFVPAKQAVLPIFDRIFTRIGASDDLIAGQSTFMVEMMEANQALRYATPN
                     SLILFDELGRGTATYDGMALAQAIIEYIHRHVQAKTLFSTHYHELTVLEKELPQLKNV
                     HVGAVEKDGEVVFLHKMMDGPADKSYGIHVAKIAGLPSNLLERAADILHTLENGDNGH
                     HTVPAEVEEKTKTAKQEQIKPPAEKVNQDHIQDRVKEENQQLSLFSELSENETEVIES
                     LKQINLLEMTPMDALNTLYELQKQL"
     gene            13868..15976
                     /gene="mutL"
                     /locus_tag="IXT20_001619"
     CDS             13868..15976
                     /gene="mutL"
                     /locus_tag="IXT20_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HAQ4860066.1"
                     /translation="MAKIQELSERLANQIAAGEVVERPASVVKELVENAIDAGSTQID
                     ILVEEAGLRTIQVIDNGEGILADDVENAFKRHATSKIHNRDDLFRIRTLGFRGEALPS
                     IASVSEMTLETANQEEKQGTYLSLKGGEVIEHRPAPLRQGTKITVSNLFFNTPARLKY
                     VKTLHTELANIGDIVNRLALSHPSIAFRLVHDGNKMLATAGNGDLKQTIAGIYGLSTA
                     KKMLKIEGKDLDFELFGYVSLPEVTRASRNYLSTIINGRFIKNFSLNKAIVAGYGSKL
                     MVGRFPIAVLEIKMDPLLVDVNVHPTKQEVRLSKEKELTELISNAIQKALSQENLIPS
                     AADNLRFKKKVPDQPKTEQIEIDLSYAFEEEKPKKPSSLNFDRETGTFFVEELKNTFV
                     DRIVDKSVENVENYSGFSEENTTYPEKTAKTVDKFGENVDNFVEKTVEKSEKKEFPIV
                     DNMEKTNEEQADKSVEEDEKIYEEELTLHPEFDFTQGNDKTLEQAIKKLETEKPVQRF
                     PQLEYFGQMHGTYLFAQSSDGLYIIDQHAAQERIKYEYFREKIGEVSDDLQELLVPFV
                     LDYPNSDALKLKEQKHLLEDAGIYLEEFGQNSFIVRAHPTWYPNGEEESIIREMIDML
                     LTTGSVSVKKFREATAIMMSCKRSIKANHYLNEAQARVLLKDLARCENPFNCPHGRPV
                     LIHFTNSDMERMFKRIQDPH"
     gene            15998..16558
                     /locus_tag="IXT20_001620"
     CDS             15998..16558
                     /locus_tag="IXT20_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation inhibitor Maf"
                     /protein_id="HAQ4860067.1"
                     /translation="MKIVLASQSPRRKELLAHIVSEFEIEPADIDETPFPDEQPVDYV
                     RRMAEEKARTVWEQDEKEDHLVIASDTTVVLNQEIMGKPEDLAEAEFMLKKLSGETHT
                     VYTAVVLKTKEREERILAEAHVTFYPLTDEEIKRYLETGDYADKAGAYGIQNAASVFV
                     KEISGDYYSIVGFPIGAVNQALKTFI"
     gene            complement(16643..18631)
                     /gene="treP"
                     /locus_tag="IXT20_001621"
     CDS             complement(16643..18631)
                     /gene="treP"
                     /locus_tag="IXT20_001621"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system trehalose-specific EIIBC component"
                     /protein_id="HAQ4860068.1"
                     /translation="MAKYQADAEKLLHDIGGKENIAAVSHCATRMRFVLNDPGKADEK
                     AIEDIPSVKGMFTNAGQFQVIIGNDVSTFYNDFVAVSGVEGVSKEQGKAAAKQNLHPV
                     QRAIAVLAEIFTPLIPAIIVGGLILGFRNVLEGIQFESLGGTIVEHSKFWNGVNAFLW
                     LPGEAIFHFLPVGITWSIAKKMGTTQILGIVLGITLVSPQLLNAYSVASTAAADIPFW
                     DFGFAQVQMIGYQAQVIPAMLAGFMLAYLEIFFRKYIPQSISMIFVPLFSLLPTVLAA
                     HVILGPIGWTVGSWISNIVNTGLTSSISWLFSAVFGFLYAPLVITGLHHMTNAIDMQL
                     IADFGSTNLWPMIALSNIAQGSAVLAIIFLHRGNKKEEQISIPAMISCYLGVTEPAMF
                     GINLKYVYPFVAAMVGSGLAGMFANLMGVRANAIGVGGLPGILAIQAETWVPFIIAMI
                     IAVIIPFGLTIIFRRQGILNKIDPAVPENAADVQLQTANGATATPQSFEPVSATGTAV
                     ATKETLFAVAAGTIKEITEVNDPVFSQKMMGDGYAVEPSNGKVYAPVNGKVTSVFETK
                     HAIGILSNEGLEVLVHMGLDTVELKGVPFNVFVKEGYLVTPETLIAEMDLPEIEQAGK
                     KTDIIVALTNNEKVAGLSLDQSGLVRPGEAVGKAEVKS"
     gene            18854..19567
                     /gene="treR"
                     /locus_tag="IXT20_001622"
     CDS             18854..19567
                     /gene="treR"
                     /locus_tag="IXT20_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor"
                     /protein_id="HAQ4860069.1"
                     /translation="MNKFNEIFLDLEQKILAKEYPPHSLLPSENQLIKIYGVSRETIR
                     KALNLLTTEGYIQKKQGKGSIVLDRNRFDFPISGLTSYKELQETQRIPSETIIHTLEE
                     TEVTKALNEITGWEIGAPVWHLIRERKINGEVVILDTDYLLKEIVPILTPAQAQGSIY
                     EYFENELSLTIAYAQKEITVEEVTEIMKTAMDLHDDTYAVVVRSLVHLEDTRCFEYSE
                     SVHRLDKFRFVEFARRRKV"
     gene            19644..20090
                     /gene="msrB"
                     /locus_tag="IXT20_001623"
     CDS             19644..20090
                     /gene="msrB"
                     /locus_tag="IXT20_001623"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HAQ4860070.1"
                     /translation="MDKPIRPEKEELKEKLSPMAYAVTQENATERPFSGKYDDFYEKG
                     IYVDIVSGEPLFSSAEKYDAGCGWPSFSKPITKRQVREKADFSHGMHRIEVRSKEADS
                     HLGHVFTDGPVDQGGLRYCINSASLKFIPYEKMDELGYGEFKSLVE"
     gene            20260..20862
                     /gene="ruvA"
                     /locus_tag="IXT20_001624"
     CDS             20260..20862
                     /gene="ruvA"
                     /locus_tag="IXT20_001624"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HAQ4860071.1"
                     /translation="MYEYLTGVVTFINPYYIVVETNGIGYQIALGNPYRYSSKLNQKI
                     KIYVHQVIREDAHLLYGFDSLEEKQLFLRLVSVSGIGPKSALAIMASEDHSGLIQAVE
                     SSDVTYLTKFPGVGKKTAQQMILDLKGKFGELSIDTPFSLFDEANTKDATALSEAMEA
                     LSALGYSDREIKRVEKQLKEVDNQTTDEYLRQALKLMMKK"
     gene            20875..21876
                     /gene="ruvB"
                     /locus_tag="IXT20_001625"
     CDS             20875..21876
                     /gene="ruvB"
                     /locus_tag="IXT20_001625"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HAQ4860072.1"
                     /translation="MTDEYLLSPESGENELSLEKSLRPQLLSQYIGQRKVKQELQIYI
                     EAARNREEPLDHVLLYGPPGLGKTTMAMVIANEMTVNIRTTSGPAIERPGDLVAILNE
                     LEAGDVLFIDEIHRLPRVVEEMLYSAMEDFYVDIMVGQGPTAHPVHFPLPPFTLIGAT
                     TRAGMLSAPLRDRFGIISHMEYYDEQDLQEIVLRSSDIFQTEIIEEGALEIARRSRGT
                     PRIANRLLKRVRDFAQVQGDGKIDRPIADQALTLLQVDQAGLDYVDQKLLRTMIELYG
                     GGPVGLSTLSVNIGEERETVEDMYEPYLIQKGFLKRTPRGRIATAYAYEHFGYDYHI"
     gene            21905..22483
                     /locus_tag="IXT20_001626"
     CDS             21905..22483
                     /locus_tag="IXT20_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HAQ4860073.1"
                     /translation="MSDTTRTKQLIKNVLIDLCQTQSFRKISVQKIANEAKINRQTFY
                     YHFTDKYDLLRWVYCEDALHYLDPQCLSLDNWEEQALKMLKAIAEKKEFYANTVLSDP
                     EILQKEFSQLIQKSFIKIFEQMDEERQLSARDKLFYARFFSYGCSGVLVNWITNEYEE
                     TPMEIAMQLFRLAKDTELYSYRLYAKEELEEQ"
     gene            22535..23017
                     /locus_tag="IXT20_001627"
     CDS             22535..23017
                     /locus_tag="IXT20_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight phosphotyrosine protein
                     phosphatase"
                     /protein_id="HAQ4860074.1"
                     /translation="MTKILFVCLGNICRSPMAEGLLRKRIALEGREAEFFVDSAATST
                     YEVGKTPHPGTKKILNQEKVDMTNMVARQITPHDFETFDWIIGMDQENVEELKRRAPR
                     SAQGKIHLFLSSIPGKEKENVPDPYYTNNFDETYHLITEGLNHWWNIWIDENKITNYL
                     "
     gene            23134..23508
                     /locus_tag="IXT20_001628"
     CDS             23134..23508
                     /locus_tag="IXT20_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4860075.1"
                     /translation="MNEETVSPMKKLTKLVTHTSYQLFDSFVNDTEEHAKNVRLQGKF
                     ILELQKAATEHSLVILQVRANRLSSKYETISGWVATKTVEKDRVMIRLQNEEQQIRMV
                     PVEQIEKITILSPAGEQERLSK"
BASE COUNT         7780 a         4285 c         5115 g         6647 t
ORIGIN      
        1 aaaaaaactt ggtacgcaat cgctgtacca agttttttta tttagctttt gattaagcca
       61 taaataataa tttatataag aatactgcta cgacaccagc acagataggt gctacaactg
      121 gtacccatgc atatccccat tgagaagaac ctttatgttt caatggaaga agtgcgtgta
      181 caagacgtgg agcaaagtcg cgggctgggt ttagtccagg acctgtagga ccaccaagtg
      241 aagcaactaa tgtccataca aggaaaccaa gtgctaagtg agctgtacca agattatctt
      301 ggaagaatgg tgcttttgta atacctaggg caccaaagaa taaaacgaat gaaccaaaga
      361 attcattgac gaaaccattg aatttagaac caacgctgtt gattgttgag aatgtaccta
      421 acacatggtt tacgtcttct gtttggtcgt agtaaggttt gtaacaagct actacgatca
      481 attgtccaat cgctgcacca atcaattgtg caatgatata aggaacaact tcagcccaag
      541 ggaatagacc attagatgct aaagcaattg taaatgctgg gttgatgtgg ttaccgctga
      601 ttccaccaaa aatcattgct ggcatcataa ccccgaatcc atacccgaca gcaatcagca
      661 tccagtcact gccatatcct tttgtacctt ttaaatctac gtttgctaca gcaccgttac
      721 ctagaacgat tagtaatgct gtaccaatca tttccgccgc aagacgggtc attagtgagt
      781 aatccataaa tacctccaaa agttataaaa atgcaaaaaa aaaagaaaca tcaacacgaa
      841 aagcatcggt gcagtgtttc gtgtcggaag gatttctgca gtcttaacaa atgtgaataa
      901 aaaaatgaat tccattactt ataagaaacc agaccaacag taaaactcca actcttttta
      961 aaagttctcc acaaatcacc aagcttaact ataaatatac caaaaaaaaa acgttttttt
     1021 tctcaaaaat tgtatcttag aaacagcatg ttttgaaact ttcagaaaag gtttgttagt
     1081 aaaatgaaag ctatacgtaa ggagtgttgt ttgatgaaat tttttcaaga gtttttcatt
     1141 attattttct tttctgtatt aggagaaggc atacggattt ttgctcattt acctattcct
     1201 ggaagtatct taggtattct ttttttattt ttagcatttg aaatgcatct tgttgatcct
     1261 gaaaaaatcg gagcaactgg tgatttttta ttgaataatc tagccatttt atttgttcct
     1321 gctggtgtag gtttattgga atattttgat gatattgctt cgatatggcc tgttttactg
     1381 ggagcagtcg tcgtgtgttc catcgtcacg atggttgcag cgggaaaaac ggcagaaggt
     1441 gtagaagctt tgctgggcta tgtccgtaag aaaaaagcag aaagggtatc tgtaaacgaa
     1501 atggaggaga atcaaattga ctgagttttt tgtgaatcca ttatttggct taagtttgac
     1561 tttaggagtg tacgtattat ctgacaaatt tttgcggaaa tataaactgc ctgttttgaa
     1621 tcctctcgta ttatcgattg ttgttattat cttatttttg catttttctg ggatcagcta
     1681 taaagactat aatatcgggg gcaatttttt gactatgctg attactcctg cgacagtggc
     1741 acttgccatc cctctttata aaaacttccg tttactgaaa gagaattttt atcctgtaat
     1801 ggctgcgatt ttagcaggaa tagtcgcaaa ttgcttggtt agtgtagggt tcggttactt
     1861 cttctctctt cataaagaga tggtgatctc attactacct aaatctgtaa cgacggctat
     1921 ttctgtcgat ttatctcata cgatgggcgg ggtgaatgcc gtgacacttg ctattgttgt
     1981 ctctacaggt atttttggtt cgctgatcgc cactcatatt ttccgcctat tcaagatcga
     2041 aagtccagtt gctcgcggtg tggcgttagg aagtacttct catgctattg gtacagcaaa
     2101 agcaatcgag attggtgaaa tagaaggaat catttccgga cttgcgatat gcgtcaacgg
     2161 aatattgacc gttttattgc taccgttatt ctttcagcct tttgctggct tgttttgatg
     2221 cgaaaaccac tattttatgc tacaataacc aaaacgttcg agcataaaaa aggagggaaa
     2281 gacgtgtatt tgtcttccga gtatcttaaa caatttgata aagatctata ttataagatt
     2341 ctcctcctag aaagttttga ggatcaagct tggcataccg ctgcacaatt agcgcaagtt
     2401 gttcagctgg atgcccgcag cgtgtcgaaa tacctgaacg agctgtctaa aaactatcag
     2461 cagtttagcg gtaagacaca ccctttgttt acaaaaaatc accgttctgg ttataacttt
     2521 tatgatacgc tcgattcaat tgaacatgag cgttttttga tctatctcgt gcaaagtacg
     2581 ctgaaattcc agttgctgca cgacattttt tttgaagaat tccatacaat gtaccaattt
     2641 gcgcaaaagc attacattag tgaatctact gctcatcgaa aaatcaatga atggaaacag
     2701 caactgcaaa cttacggaat tcggctgcaa cgaggtacat atatcgctca aggcgaagaa
     2761 gaaatcattc gactttactt gcatatgaca ttctggcagt tgtttcgagg gaaaatctgg
     2821 ccatttgaaa cgatttctca aatggacgta aagaatatgg cagaacatat catggctttt
     2881 tttaatgttc gtctgaacga gatcaaaaaa agacgattgg aatatatgct aggtgccttt
     2941 tttttaagaa aatcacagaa gcattatgtt gtattgaatg agaaaaaaag acggctgatt
     3001 tctgacaatc tgttgttcca gcgattttgc caggtcatgg agccggtatt tcctaattat
     3061 tttcaagtag aagacgagtt aggcgcatta tttcttgtac tgatgactcg tgaagaatat
     3121 tatagtgatc ctaagatacg gaagaaaatt tttgattttc atcaacaagc aaaaacaccg
     3181 ccctttacag ccctttcaga agcaaaagcc gctctttctc tttatcagga agagcagggg
     3241 ctgccagcag aaaatctaac cttcgaggca gaaaactatt tattcagcag tcattttttc
     3301 gcctatcttt ttcctaatgc gaaagaaacg attgatggca atagtagtga ttttattaat
     3361 catttagtta ttgaaaacaa ggaattgaaa caatggctcg tacatttttt tgaaagtaga
     3421 cacaaacatc ccaatcatct ggcatttaaa aatcatgcgt ttttgatggg gcggtatctg
     3481 actgtgttta aaacattagg agcctttact ccacagcttc caaagatcac gatcctttta
     3541 atgacagatt tcccattatt tgaagagcaa ctgttagagg agggattacg gacatttttc
     3601 cgtaatgagt atcagctgat ttttctgcca acagattatc gggggagaga agttgatctt
     3661 ctgatttcga caagtaaagt ccaccgcaaa ccatgggcgg atcttgacta ttttattgtg
     3721 actgaagaac taaaattaat tgattacatc caactatctc aaaaatttga aatgattcaa
     3781 aaacaaaaac tattaaaaca ataacaaaga aggagaaaaa agatgacatt tgaagaaatt
     3841 ttaccagaat tgaaaaaagg tgcaaagatt attcgtaaag gatggagtgg atttgaactt
     3901 tatgttgttc tcgtatccga tgatgaattt gatggctgtc cggtgacgcc gtacttattg
     3961 atcaaaacaa gtgacgaagg tttttccagc ttcgctccta cagtttgtga tattttagca
     4021 gatgattggg cgatcgtaga atgaaaaacc agtatccaga tctagctaaa caggttgtat
     4081 ttatcacagg agctgcttct ggaattggtg ctgcgcaagc ccgagcgttt cttgaacaag
     4141 atgcttttgt atttggatta gatcaaaagt tcggtaaaat ggaagaaatt tctttgcgat
     4201 ttcctgaaac ttttgcctac gcactaggtg atgttcgaca aatgaaagaa ctccaacaag
     4261 cggtatcgaa atgccaaaat cattttggtg aagttacgat tttactgaat acagcgggga
     4321 tattggatgc ctacaaaccc ttgatggaaa ctgacgaatc gctgtgggat ctgatttatg
     4381 agacgaatgt gaaaagcatg tatcagctga caaaaatcat ccttcctgat atgatccgcc
     4441 aaaaaagcgg aacgatcatc aatatggctt cgattgctgg cttgattgct ggtggaggtg
     4501 ggatcgctta tacaagcgcg aaacatgcga ttgtcggatt taccaaacaa ctcgctctgg
     4561 attacgcagg acagggtatc cgagtaaaag gaattgctcc aggagccatc caaacaccaa
     4621 tgaatgcggc tgattttgaa gggaaaggtg agatggcaga gtgggtggca aatgaaacac
     4681 cagtcaagcg atgggcacag ccggaagaag tagctgagct gacgttattt cttgcctctc
     4741 ctcaagcaag ctacatacaa ggaacgatca tcccaatcga cggcggatgg ttgttaaaat
     4801 aaccttgtgt ttttttcaag cttctgtatg ataaaactaa agagagaaac aacggttgat
     4861 gataaaaaaa gtcgatgact gttcccgaca ttcgatgccg aacagccagt tcaaaaaatt
     4921 ctttttaata atcgttaata ttcgaaacta agcggctttt cacgacctct tgatataaac
     4981 gatattcaaa agcttgcaac tgcggtaaca agtccagcca aatgaaaaat ataaggagct
     5041 atttttattt gaccattctt attacttgtt gcagggcagc tttttcacta ccttctgcga
     5101 cacttcagtt ggaattttct tcccatcctt tggtcgcggt cattaccaag ataaaattat
     5161 cttgcaaagg agaaatagaa catggaaaaa aataataccc gttcagccaa acgctggaca
     5221 acgacagatg tgactcggat ggcgctagtc actggtttat atgtggcagt gacgctggtt
     5281 ttgtctgtct ttagctttgg agtgattcag attcgtctct cggagatgtt caactatctg
     5341 tccttgtata acaaaagata catcgtcgcg gtaacagcgg gagtagcttt ggctaatatt
     5401 gcttctcctc ttggactgat agatgttgca gtaggaagcg tcagcacgct gcttgtattg
     5461 cttatcaact ataaaatcac tagtcgcatc aaaaacatga aaatcaaaat ggccgtgaca
     5521 gcatgcgtat ttgctttttc catgtttaca gtagctggtc agctaacgat tttatatcaa
     5581 ctaccttttt tcttgaattg gtgggtgatt gctttaggag aattggcatc aatgattgtc
     5641 ggtgggataa ttatttattg gatcggtaaa aaagtggatt tgtcaaaata aagatctgtt
     5701 taatttaaaa tagggcagca tggaagagac tgaatgttga gtcttcttcc atgctgcttt
     5761 tttgattaat ttgtatgaag aaatcgattt agccgttttt tcatcaagat accgatataa
     5821 gctgctccag ttgccttgat tgcttccggc agtaaaaacg gcagtagtcc actggcaaat
     5881 gcagttgtaa aagtaagatc accacttatt ttcagccaga tcgttccgca gatcaatgca
     5941 gcaagagagc ccattagatt ggcaaagatt gcatataagt aatgagcatc cgttctttcg
     6001 atcatccaac cagttatcca cgtttgtaag agaaatccaa tcaaatatcc gccagttggc
     6061 ccaaataaaa cagctatacc tccagtcatc cctgaaaata caggaagacc aatcaagcca
     6121 agtaaaagat aaactccaac agatatcaat cctagttttc ttccgagaaa agttgctgtt
     6181 aatccaatag caaatgtttg ccctgtcaaa ggaaccaaac caaaaggaat ggtcatctga
     6241 gaaaggacag aaataatcgc ggcaaattcc gcagcaagta tcaattcttt caatgttcgg
     6301 ttcataacgc cctcttttcg ttaacttaat atttttattt ggttaacgaa attgtaaaac
     6361 aaagtgatta ctttgtaaat agaaaatata aaaaaagacc agctatccaa tagatagcgg
     6421 tctttttcaa gaaagctttt gaaattataa gctttggttg tagtattcaa cgacaagagc
     6481 ttcgtcgatt tctggatata gttcgtcacg ttctggtaaa cgagtcaatg aaccttccaa
     6541 tttttcagcg tcaaagctta cgaatgctgg gcgtccaaca gttgcttcta cagcttcttt
     6601 tactgttacg atgttttgtg atttttcacg aactgagatc acttgaccca cttctacgtg
     6661 gtatgaaggg atatcaacgc gtttgccatc aactaaaatg tgaccatggt taactaattg
     6721 acgtgcttga cgacgagtcg ttgcaagtcc taaacggtaa acaacattgt ctaaacgttg
     6781 ttctagtaag atcatgaagt taacaccatg tttaccttct ttgattttgc tagcttttac
     6841 gaacaagtta cggaattgac gttcgttcat tccgtacatg tgacgaagtt tttgtttttc
     6901 agttaattgc atgccgtatt ctgattgttt gccacggcta ttaggtccat gttgacctgg
     6961 tgcgtaaggg cgacgtgcta attcttttcc tgtgcctgac aatgagatgc ctaagcgacg
     7021 agaaattttc catgatggtc ctgtataacg agacattaaa atttcctcca atgaatataa
     7081 gtttttggag taaaataatc cgttgaaaaa ttcataatca tatagtttgt tcttcaatct
     7141 tcacctttgc agccgcggtt acacaatcga acccgagacg gggcaacaaa ctagggatga
     7201 gttattattt ttctgctgca ttattttaca caaattacag tatatgatag atcatccacg
     7261 aatgtcaaga taattagtac ataaaaacgc tttacaaacc taggctgtct ttataaaatg
     7321 agagggtatg tgcaaatgga aaaaagcgaa ggaagtttaa ggagaaaatc aatgaatccg
     7381 gtatcaccat tatttgagaa aatcgatgct tcaattgaaa agaaggcgaa tctttttgaa
     7441 aacagctttt cacgttacgc tgttcgagcg gttctcgcct gtttgttttt gactttagga
     7501 acggctgtgg catttgcgat cgcgatgaag ggggaagata tcgcgcatgg tctaggaaag
     7561 attttatacg catttatgtt cagctggggt ctggtcatga ttctatacat gaacgccgaa
     7621 ttaggaacat cgaatatgct ttatatgacc gtgggtgttt ttcggcagag acttgcttta
     7681 agtaaggcag ccaagattct atttgcctgt atttttttca atatgattgg cggtatatta
     7741 tgtggatttc taatttctat gactacgcca tttcaagatt taacgttgga caactttatg
     7801 ttagaatcaa tagcagtaaa actagcaaaa ccttcagcaa cgattttggt ggaagggatg
     7861 tttgcgaata tcgttgtcaa taccgctgta ctgatcagca tgcggatgaa agaggatgtc
     7921 ggaaaagtga tcacagtcgt attcatcatt ttcatttttg cttttttagg atatgaacac
     7981 gtgatagcga attttcctgc ctttacgctt acttactttg cttctcacgg acagatggat
     8041 ggaatgacag tcagcaatgt gttacataat ttgttttttg ctttggcagg aaattatatt
     8101 ggtggcggtt tagtaatggg actgggatat gcttggctga atcagtcaaa atcatcctat
     8161 gtggactaaa aagtcgttca ttaaagggga agtgccatgg aagttttagt ttttccaaaa
     8221 aatgaggaaa aagcgttaca aatggaaaag tacatgaaaa atcttttttc ctttgctgga
     8281 gtacctaagc cggaacgcgc tcttttacaa caaccgttac tggatgagag caaaaattta
     8341 tctatttctg aagttttagc ttttgttcat tattattatg aacaaatcga aagagagtat
     8401 cagtatgtag cgatcgattt agtccagaaa aacatcaaac ggcttacatt tgaagactta
     8461 gtcgtattgt tgccactagt tcagaaaaaa gaatggtggg acagcatcga ctcttggaga
     8521 aaagtataca gcacatggtg taaagcctac ccagagcagc gtgaagaagt ctttcaattg
     8581 tttgaaaaaa aggaagattt ttggctacgt cgaatagcaa tcacgttgca attaggttat
     8641 aaacagcaaa cagacacagg actattgatc cgagcaatag acgaagatcg tcaaacaaag
     8701 gaattcttta tccaaaaagc aattggttgg gcattacgcg attatagtaa gaccgatgcc
     8761 gattgggtaa aaagacagct gacaacagat ttgagtaagt tagctgttcg agaggcaagt
     8821 aagtatttat aagaaagcga gacgacaaga gatgagacgc aaattatttg cttttgatat
     8881 tgatggaacg ttgttagatt caaacaaaca agctttagat agcacaagag aagcattgga
     8941 aaaattacgc aaagccggtc atctggtcac gattgctaca gggcggagcc gatttcatgc
     9001 gcaagagatc attcgcgatc ttgattttac gaactacatt ttatgtaatg gagcggccgg
     9061 ttttttagac catgaacaag tatataagaa tttactagat cgcgaccagt tggatgcgtt
     9121 cgttcaagaa gcgcatcatg agcagatcga tacagctttt gtcagcttag acaatatcaa
     9181 gcgttcttct tccaatgatg taacggtgat ggaagaagcg atgcattctt tcggtgctgt
     9241 cttgcctgaa ttagatcagt tttttgcaga ggaacaagaa atataccaag cgttagcatt
     9301 tttcgatcaa acatatgatg ggaagttctc ttcatatgat aaaattcgat ttgtccgctg
     9361 gcatgagaac agtgtagatg tcgtacctaa aaatggttct aaagcagcga caattctgca
     9421 cttagcagaa caagtaggca tccctcatga agacatcatc agctttggcg atggacaaaa
     9481 cgaccgtgaa atgttgcgca tgtcaggaat tgggatagct atggggaatg ctgtgccaga
     9541 agtacaagca gaagctaaga tggtcactga tacaaacgat caggatggaa tttggaaggc
     9601 attgaaagag ttaagcgtga tttaaaagag gttgtgaaaa agctgttctg catcaagtaa
     9661 taagcccagc caaacaaaaa tagctcctcc tatttttcgt ttttctgggc ttattaccgc
     9721 agatgcaagc tttcgaacac cgtttaagga agaggtcgtg acagaagtgg tcagtttcaa
     9781 gaaataagaa gggatttacg aaaattgctt ctcaaatttt tgtaaaatta cggcttattt
     9841 ctgaagaagt agatgaaaca acgtttattc gggaaatggg actgtgacaa gatgtttgtc
     9901 atagtcccat ttttttattt tatttttcaa aactaagaaa taccaggaaa gagttccttt
     9961 tgtttgacct tttctttctt attgaaaaca actataatat agagtgatgt taaaagagaa
    10021 gaggaattta gtgtgcgggt attgtttatt ggtgatgtag taggttcaat gggacgagag
    10081 atgattactg aatatcttcc tagattgaag aagaagtacc gtccgcaagt aacgattgta
    10141 aatggtgaga atgccgcttc tggacgtgga atcacagaaa aaatctataa aaaatttttg
    10201 caagacggcg tagatgtcgt gacgatgggg aatcatacgt gggacaaccg cggaatcttt
    10261 gagtttgtaa atgaagctaa aaagatggtt cgtccggcaa attttccgga agggacgcct
    10321 ggacaaggga tggtctttgt caaagtgaac caagtcgaac tggcagtgat caacatgcag
    10381 gctcgttctt ttatggtgga cttggatgat ccttttcgta aaatgaatga gctggtagaa
    10441 gaagccagaa aacgtacgcc gatcattttt gtagattttc atggagaaac aacaagtgaa
    10501 aaacaagcaa tgggctggtt tttagatggg aaagtttcag cagtggtcgg tacacataca
    10561 cacgttcaga caaatgatgc gcgaattttg ccaagaggaa cagcgtatct gacagacgtt
    10621 gggatgacag gtccctatga tggtattttg ggtatgcgca gagaacctgt aatcgaaaag
    10681 tttctgactg cattaccaaa acgttttgaa gttgtggaac aaggccgatc gattttaagc
    10741 ggctgtatct tggaattgga tgatacgaca ggtcatgcaa aagaaatcca gctgattcag
    10801 atcaacgatg atcgaccgtt catggagtag aaagagaggc agttttttgc agcaggagcc
    10861 ggaaattcaa aaagaagtaa ggaaactgac aaaactttta agagagaatg aaacgatcat
    10921 tcgctacaaa gaacttgaag aaaagatcca gcaaaaccag tatttagctg agttgagaga
    10981 aaaaatcaaa caagcgcaaa aagatgctgt tcattttgcc cactatgaca aaccagcagc
    11041 agaaaaagaa gcaatcaaac aagcggatca attcatgcaa gaatttgatc agcatccgct
    11101 tgttgtagct tatagaaaac agctgttgga agcagatgat ctgcttcatc atttaacaac
    11161 tatgatacaa gaagaaatca atggacaaat agaggaggaa aaacatgcct caaaaaacta
    11221 aaaatactcc tatgatggaa caatatctaa gcatcaaggc ccaataccaa gatgcttttt
    11281 tgttctatcg tttaggagac ttttatgaac tattttacga agatgcgatc aaagcgtcac
    11341 agcttttgga attaacgttg acgagcagaa atcgaaatgc agaagaacct attcctatgt
    11401 gcggtgtacc tcaccatgca gcacaaggat atatcgatgc cttgattgaa aaaggctata
    11461 aagtagcgat ttgcgagcag gtagaagatc caaagacgac taaagggatg gtcaagcgcg
    11521 aagtgatcca gctagtcact cctggtacag tcatggacag caaaggattg tcggcaaaag
    11581 acaacaattt tttaacagct gtagttagtc aaggaaatga atttggcttt gcttatgccg
    11641 atttaagtac gggagaattg aagacggctc ggttgcatga tgaagaagct gttttaaatg
    11701 aagcctctgc attgcagaca aaagaactcg tattaggcag tgagatcact gatacccttc
    11761 gtgaacagct aagcgggcgt ttagggctag tcttttctca acaaaatgag atggaagaaa
    11821 acgcggaatt tagctttttg acaagcgaac tagtcgatcc gctggaaaaa gaagcaaccg
    11881 gcaaactatt gacttattta tcggtgacac aaaagcggag tttagctcat atccaaaaag
    11941 cagtagaata ccaaccagat cattttttga aaatggatta ttattcaaaa ttcaacctag
    12001 aattaagcca atctattcgg actgggaaaa agcacggcac tcttctatgg ttgttagatg
    12061 aaacaaaaac agcaatgggt ggccgtttac taaaacaatg gctagaccgt ccgcttatcc
    12121 aggcaaaaca aattaaagca agacaagaaa tggtcgcttc tttgctagac agctattttg
    12181 aacgtatcga tttgcagtca gcattaacga aagtctatga tctggaacgc ctagcaggtc
    12241 gagtagcttt cggtaatgtc aatgggcgag acttgatcca gctgaaaact tctttagaac
    12301 aagtgccaat gatcagaggg ttgattgaag gaatcgatca aggtcaatgg caaagtctgc
    12361 tttcagaaat gcagccgatg gaccatctcg tccaattgat cgaccaagct atccaagatg
    12421 atccgccgtt acaaataacg gaaggaaaca tcatcaaaga tggattcaat gagcagctcg
    12481 atatgtaccg ttctgctatg agaaacggaa agaaatggtt ggcagaactt gaagcaaaag
    12541 agcgtcaaga aacaggaatc aagaacttaa aaatcggatt taaccgcgtt ttcggttatt
    12601 atatagaaat tacaaaagca aatcttggaa atgcagaact ggagaagtat gaacgaaaac
    12661 aaactttagc aaatgcagag cgtttcatca caccagaact aaaagaattg gagacacaaa
    12721 tcctcgaagc agaagaaaaa tctgttgatt tagaatatca gttgttttta gctgtgagag
    12781 aagaagtcaa aaaggctatc cagccgctac aagtacttgc aaaagcaatc agtgcagtag
    12841 atgtgctaca aagttttgca acgatcagcg aacgctatca gtatgttcgg ccagaacttg
    12901 tcagtgacaa gcatcagctt ctgatcaaag acggacgaca tcctgtagta gaaaaagtat
    12961 taggacatca ggaatatatc cctaacagcg tagaaatggc agaagacgaa atgatcttgc
    13021 tgattacagg accgaatatg tctggtaaaa gtacgtatat gcgacagttg gcattaactg
    13081 ttctgatggc gcaaatgggc tgtttcgttc cggcaaaaca agcagtttta cctatttttg
    13141 atcggatttt tacccgaatt ggtgcaagtg acgacttaat tgctggacaa agtactttta
    13201 tggtagagat gatggaagcc aatcaagccc tgcgttatgc tacgccaaac agtttgattt
    13261 tgtttgacga actagggcga ggaacagcaa cctatgacgg aatggcgttg gcccaagcaa
    13321 tcatcgaata tatccatcgt catgtacaag caaaaacact cttttctact cattatcatg
    13381 aactgacggt acttgagaaa gaacttccgc agcttaaaaa tgtccatgtt ggagccgttg
    13441 aaaaagacgg agaagttgtc ttcttgcata aaatgatgga tggacctgcg gataaaagct
    13501 acggcataca tgtggcaaag atcgcaggat tgccttccaa tctactagaa cgagcagcag
    13561 atatcctgca tacgctagaa aatggcgata acggacacca tactgttcca gctgaagtag
    13621 aagagaaaac taaaacagcc aaacaggagc aaataaaacc acctgcagaa aaagtaaatc
    13681 aagaccatat acaagaccgt gtaaaagaag aaaaccaaca gctttcttta ttcagtgaat
    13741 tatccgaaaa tgaaacagaa gtcattgaat cactgaagca aataaattta ctagaaatga
    13801 cgccaatgga tgcactaaac acattatatg aattacaaaa acaactgtag gaagaaggcg
    13861 gatatcgatg gccaaaatcc aagaattatc tgaacgttta gctaatcaga tcgctgcggg
    13921 agaagtagtg gagcgaccag cttccgttgt gaaagaatta gtggaaaatg caattgacgc
    13981 cggcagtact cagattgata tcttagttga agaagcggga ctgcggacga tccaagtgat
    14041 tgataacgga gaaggcattt tggcagacga cgtagagaat gccttcaagc ggcatgcgac
    14101 aagtaagatc cataatcgcg acgatctttt tcgcatacga acgctgggct ttcgagggga
    14161 agctttgcca agtattgcct ctgtttctga aatgactctg gaaacggcga atcaggaaga
    14221 aaaacaaggc acctatctgt cgctgaaagg cggagaagtt attgagcatc gaccagcacc
    14281 actgcgtcaa ggaacgaaga ttaccgtcag caatctgttt ttcaatacgc cagcccgctt
    14341 gaaatacgtg aaaacactgc acacagaatt agcgaacatc ggggacatcg tcaatcgact
    14401 ggcattgagt catccatcga ttgcttttcg tttagtacat gatggaaaca aaatgctggc
    14461 aactgctgga aatggcgatc tgaaacaaac aattgcggga atctacggat tatctaccgc
    14521 aaaaaaaatg ctgaaaatcg aaggaaaaga tctagatttt gagttatttg gctatgtttc
    14581 attgcctgag gtgactcgtg ccagtaggaa ttacttatcg acgatcatca atggacgttt
    14641 tatcaagaac ttttccttga ataaagcaat cgttgcaggt tatgggtcaa aattaatggt
    14701 cggtcgtttc cctattgctg tattggaaat caaaatggac ccgcttttag tagatgtaaa
    14761 tgttcatccg acgaagcagg aagtccggct gtcgaaggaa aaagaactaa cagagctaat
    14821 tagtaacgcc attcaaaaag cattgtctca agaaaatctg attccaagtg cagcggataa
    14881 tctacggttt aagaaaaaag tacctgatca gcctaaaaca gaacaaatcg aaatcgattt
    14941 gtcctacgct tttgaagaag aaaaacctaa aaagccaagt agtttgaatt ttgatcgaga
    15001 gacaggtacc tttttcgtag aagaactaaa gaacacattt gttgacagaa ttgttgataa
    15061 gtctgtggaa aatgttgaaa actattctgg tttttctgaa gaaaacacca cttatccaga
    15121 aaaaacagcg aaaacggtgg ataagtttgg tgaaaatgtt gataactttg tagaaaaaac
    15181 agtggaaaaa tctgaaaaga aagaatttcc cattgtggat aacatggaga aaacaaatga
    15241 agaacaagca gataaatccg tagaagagga tgaaaagatt tacgaggaag aattgacact
    15301 ccatcctgaa tttgatttca ctcaaggaaa cgacaaaaca ttagaacaag ccatcaagaa
    15361 attagaaacg gaaaaaccag tacaacgttt tcctcagttg gaatattttg gacagatgca
    15421 cggaacgtat ctttttgccc aaagtagcga tgggttgtac atcattgatc agcatgctgc
    15481 ccaagagcgc attaagtatg aatatttccg ggaaaaaatt ggggaagtat ctgatgatct
    15541 gcaagaattg cttgttccat ttgtgttaga ctatccaaac agtgatgctt tgaaattaaa
    15601 agagcaaaaa cacttgctag aagatgcagg gatttattta gaagaatttg gtcaaaacag
    15661 ctttattgta cgggcccatc ctacttggta tcccaatgga gaagaagaat cgattattcg
    15721 ggagatgatc gatatgctgc tgacaactgg aagtgtgagt gtaaaaaaat tccgtgaagc
    15781 aacagcaatc atgatgagtt gcaaacgatc gattaaagcc aaccattatt tgaatgaagc
    15841 acaagcacgt gtgctgctga aggatctggc acgatgcgaa aatcctttca attgtcccca
    15901 tggccgtcca gtattgattc actttaccaa ttcggatatg gaaaggatgt tcaaacgaat
    15961 ccaagacccg cattaagtca aagagaagag ggaaaacatg aagattgtat tagcatccca
    16021 atcaccaaga agaaaagagt tattggctca tatcgtttca gaattcgaga ttgaacctgc
    16081 tgatatcgat gaaacgccat ttccagacga acagcccgtc gattatgttc gtagaatggc
    16141 tgaagaaaaa gcacgaactg tctgggaaca agatgaaaaa gaagaccatt tagtgattgc
    16201 cagtgacacg actgttgttc tcaatcaaga gatcatgggt aaaccagaag atctggctga
    16261 ggctgaattc atgctgaaaa aactaagcgg ggaaacacat actgtatata cagctgttgt
    16321 tttaaaaaca aaggaacgag aagaacggat actagcagaa gcacatgtca ctttttatcc
    16381 tttgacagac gaagagatca aacgctactt ggagacgggg gattatgcgg ataaggcagg
    16441 ggcatacggc atccaaaatg ctgcgagtgt attcgtcaaa gaaatttctg gagattatta
    16501 cagtatcgta ggatttccga ttggtgctgt caatcaggca ctgaaaacat tcatttaaac
    16561 aaaaaagcgg actatctgat tttcgattga aaatccatga tagcccgttt tttttattga
    16621 aaagattact tatctactct gactaagatt ttacttcagc tttaccgact gcttcgcccg
    16681 ggcgaacaag tcctgattgg tcaagagaaa gtcctgcaac tttttcatta ttggtcaaag
    16741 caacaataat gtcagttttc ttgccagctt gttctatttc tggcaaatcc atttcagcaa
    16801 tcagggtctc tggtgtgaca aggtatcctt ctttcacgaa tacattgaaa ggaacaccct
    16861 ttagttcaac agtatctaat cccatgtgaa caagtacttc cagcccctca ttagaaagaa
    16921 taccaatggc atgttttgtt tcaaagacac tcgtcacttt accgtttact ggtgcataga
    16981 ctttgccgtt tgacggttcg acagcataac cgtcacccat cattttttgt gagaaaactg
    17041 ggtcatttac ttcagttatt tccttgatgg tccctgcagc gactgcgaat aatgtttctt
    17101 ttgttgcaac tgccgtacca gttgctgaaa caggttcgaa agactgtggc gttgctgttg
    17161 cgccatttgc tgtttgaagt tgtacatcag cagcattttc aggaacagca ggatcaatct
    17221 tattcaaaat tccctgacgt ctaaagatga tcgtcaagcc aaatggaatg atgacagcaa
    17281 tgatcattgc gatgatgaaa ggtacccatg tttctgcttg gatagctagt attcctggca
    17341 aaccgcctac tccgatcgcg ttagctcgaa cacccatcaa gttagcaaac atgccagcta
    17401 atccagagcc gaccattgca gcgacgaaag gatagacgta tttcaaattg attccgaaca
    17461 tagcaggttc agttacgcct aagtaacaag aaatcattgc tggaatcgag atttgttctt
    17521 cttttttatt cccacgatgt aagaagatga tggctaagac tgcagagcct tgggcgatat
    17581 ttgataaagc aatcatcggc cataaattcg ttgatccgaa atctgcaatc agctgcatat
    17641 cgatggcatt ggtcatatgg tggagaccag taatcactaa aggagcatac aggaatccga
    17701 atacggcact aaacaaccag ctgatagaag acgtcaatcc agtatttacg atatttgaga
    17761 tccagcttcc gactgtccag ccgataggac ctaaaataac gtgtgcagct aaaacagttg
    17821 gtaacaatga aaacaatgga acaaaaatca tcgagatcga ctgagggata tatttgcgga
    17881 agaagatttc tagatacgct aacataaacc ctgccagcat ggctggaata acttgcgctt
    17941 gataaccaat catctggact tgagcaaaac caaaatccca aaatggaata tctgctgcgg
    18001 ctgtggaagc tacactataa gcatttaata attgcggaga aactaacgta attcctaaaa
    18061 cgattcctaa tatttgtgta gtacccatct tttttgcaat actccaagtg atacctactg
    18121 gaagaaaatg gaaaattgct tcacccggta accacaaaaa ggcattgaca ccattccaaa
    18181 acttcgagtg ttcaacgatt gttccaccaa gactttcaaa ctggatacct tcaagtacat
    18241 ttctgaatcc aaggatcaat ccaccgacaa tgattgcagg aatcagtggt gtaaagattt
    18301 ctgctaaaac ggcaatagca cgttgaacgg gatgaaggtt ttgttttgca gctgcttttc
    18361 cttgttcttt ggaaacccct tcaactccag atactgcaac gaagtcgtta taaaaagtag
    18421 aaacatcgtt tcctatgatg acttggaact gtcctgcatt cgtgaacatc ccttttacgc
    18481 taggtatatc ttcgatcgct ttttcgtccg cttttcctgg atcattcagg acaaaacgca
    18541 tacgagtcgc acaatgacta actgcagcaa tattttcttt tccgccgata tcatgtaaca
    18601 gcttctcggc atctgcttga tattttgcca tatcaataaa cccctttctc ttaacttgtc
    18661 tatgcaagtt gtttcttcat ttactatact ttttttcgga gagaattgca agcgttttat
    18721 taaaaagtac gagagaaaat gatatcgttt taaaaagctt aaaataagct tttcttaaaa
    18781 ataggaacat tgaaatacaa aaagagctgt agtatagtga agaaatgatg gaagaacgag
    18841 tgagggaaaa aacatgaata agttcaatga aattttttta gatttggaac agaaaatttt
    18901 agcgaaagaa tatcctccgc attcgctttt acccagtgag aaccaattga tcaaaattta
    18961 tggtgtttca cgagaaacaa ttagaaaagc gctgaatctt ttgactacgg aaggttatat
    19021 tcaaaaaaaa caaggaaaag gttcgatcgt actggatcgg aatcgcttcg actttcctat
    19081 ttctggattg actagctata aagagctaca ggagacccaa cgtattccaa gtgaaacgat
    19141 catccatacg ttagaagaaa cagaagtgac aaaagcattg aacgagatca ctggctggga
    19201 gatcggtgcg cctgtttggc atttgatacg cgaaagaaaa atcaatggcg aagtagttat
    19261 attagacacc gattatctat taaaggaaat cgttcctatt ttaactccag cacaagcaca
    19321 aggatcgatc tatgaatatt ttgaaaatga gctttctttg acgattgctt acgcgcaaaa
    19381 ggagatcaca gtagaagaag taacggaaat catgaaaaca gcgatggatc ttcacgatga
    19441 cacttatgca gtagtagtca gaagcctcgt tcatctggaa gatacccgct gtttcgagta
    19501 ttctgagtcg gttcaccgat tagataaatt ccgtttcgtt gagtttgccc gacgccgaaa
    19561 agtgtaaata gttcaaaaga aagcaaattg ttggcatctg tccactttgg cgctatgata
    19621 ggatcacaaa gggggcattt ttaatggata aaccaataag accagaaaaa gaagaactga
    19681 aagaaaagtt atcacccatg gcttatgcag tcacacaaga aaatgcaaca gaacgaccat
    19741 ttagcgggaa atatgatgat ttttacgaaa aggggatcta tgtagatatc gtcagcggtg
    19801 aaccgttatt cagctctgca gaaaaatatg atgcaggttg tggctggcca tcattcagca
    19861 aaccaataac gaaaagacaa gtgcgcgaaa aagccgattt ttctcatggc atgcatcgaa
    19921 tagaagttcg aagcaaagaa gcagattctc atctcggaca tgtgtttacc gatggaccag
    19981 tagatcaagg tggtctacgt tactgcatca attctgcctc actgaaattc attccatatg
    20041 aaaagatgga tgaattaggt tatggggaat tcaaatcact tgtagaataa tggaaataca
    20101 caaagagcgg tacaatacgg ggatttactc gatcgtccgc tcttttttta ttcaagcgat
    20161 tgctatttga aagaaacgct ccctagactc cttttttcac aaggtgaaat gtgctacaat
    20221 aaagggaacg tatgtgtgca aaagaaagag gactagaata tgtatgaata tctgacaggt
    20281 gtggttacct ttatcaaccc ctattacata gtagtagaaa ccaatggtat cggctaccag
    20341 atcgcactag gaaatcctta ccgctacagt agcaaactaa atcaaaaaat aaaaatttat
    20401 gtccatcagg taatacgtga agatgcgcat cttctgtatg gatttgattc gctagaagaa
    20461 aagcaactgt ttttgcgatt agtaagtgtt tcaggaatcg gtccgaaaag tgcccttgca
    20521 atcatggcaa gcgaagacca ctccggtttg attcaagcag tagaatccag tgatgtcact
    20581 tatttgacga aatttccagg agtagggaaa aagacagcac aacaaatgat cttggatcta
    20641 aaaggaaaat tcggtgagct gagtatcgat acacctttta gtctatttga tgaagcaaac
    20701 acaaaagacg ctacagctct atctgaagca atggaagctc tctcggctct tggttacagt
    20761 gatcgagaaa tcaaacgtgt agaaaaacaa ttaaaagaag tagataatca gacaacggat
    20821 gagtatctac gccaagcact caagctaatg atgaaaaaat aaggaggaga ccaaatgaca
    20881 gatgaatact tgttatcacc agaatctggc gaaaatgaac tgagtttgga aaaatctctt
    20941 cgaccacagc tgctttctca atatatcgga cagagaaaag tcaaacaaga acttcagatt
    21001 tacatcgaag ctgccagaaa tcgggaagaa cctttagatc atgttctgtt gtacgggcct
    21061 cctggattag gaaaaacgac gatggccatg gtcattgcta atgagatgac cgtaaatatt
    21121 cgtacgacga gtggccctgc tatcgaacgt ccgggtgact tagtagcaat cttgaatgaa
    21181 ctggaagcag gggatgtcct gtttatcgat gagatccatc gattacctag agtggtagaa
    21241 gagatgcttt attcagctat ggaagatttc tatgtagata tcatggtagg acaagggcca
    21301 accgcacatc cggttcattt ccctcttcct ccctttactt tgattggcgc gacgacacgg
    21361 gcaggtatgc tttctgcacc tttacgagat cgatttggca tcatttctca tatggaatat
    21421 tatgacgaac aggatctgca ggagatcgtt ttacgttctt cggatatttt tcaaacagag
    21481 atcattgaag aaggggcatt ggaaattgct cgccgatcac gaggaacgcc tagaatcgct
    21541 aatcgcttgc tcaaacgggt tcgtgatttt gcgcaagtcc aaggagacgg gaaaatcgat
    21601 cgtcctatag cggatcaggc acttactttg ctacaggtcg atcaggctgg attggattat
    21661 gttgaccaga aattgttgcg aacaatgatc gagttatatg gcggcggacc agtagggtta
    21721 agtacgcttt cagtaaacat aggagaagag cgtgagacgg tagaagatat gtatgaaccg
    21781 tatctgatcc aaaaaggatt tttaaagcgt acacctagag ggcgaattgc tactgcttat
    21841 gcctatgaac attttggtta cgactatcat atatgatttt tgcttttaga ttggaggaaa
    21901 atgaatgagt gatacaacac gtacgaaaca attgatcaaa aacgtattga ttgatttgtg
    21961 ccaaacgcaa tcttttcgaa aaatcagcgt ccaaaaaatc gcaaacgaag caaaaatcaa
    22021 tcgacaaaca ttttattatc attttacaga taaatacgat ttgcttcgtt gggtctattg
    22081 tgaggatgca ttgcattatt tagatccaca atgtctttca cttgataatt gggaagagca
    22141 agcattgaaa atgctgaaag caattgctga gaagaaagaa ttttatgcaa atactgtgct
    22201 ttctgaccca gaaattttac aaaaagagtt ttctcaactg atccaaaaat catttatcaa
    22261 gatttttgaa caaatggatg aagaaagaca actttctgca agagacaaac tattttatgc
    22321 tcgttttttt tcttacggtt gcagtggtgt attagtcaat tggatcacga atgaatatga
    22381 agaaacaccg atggaaatcg ctatgcagct tttccgttta gccaaggata cggaattata
    22441 ttcttatcgt ctttatgcaa aagaagagtt ggaagaacag taatagaaaa attttttgtt
    22501 ggaaaagcaa taatcgctaa aaggagatgg ggaaatgaca aaaattttat ttgtttgttt
    22561 gggaaatatt tgccgttcac cgatggcaga aggcttgctg agaaaacgaa tagcattaga
    22621 aggaagggaa gcagaatttt ttgtggattc tgctgctaca agcacgtatg aagttggaaa
    22681 gacaccgcac cctggaacga aaaaaatatt aaaccaagaa aaagtagata tgacgaatat
    22741 ggttgctcgg caaatcacac cccacgattt tgaaactttt gattggatca ttggcatgga
    22801 tcaagaaaat gtagaagaac tgaaacgaag agcaccacgc tcagcgcagg ggaaaatcca
    22861 tttgttttta agtagcattc ctggaaaaga aaaagaaaat gttcccgacc catactatac
    22921 caataacttt gatgagacat atcacttgat cacagaaggc ctgaatcatt ggtggaatat
    22981 ttggattgat gaaaataaaa taacaaatta tttgtaaagt taacgctttc tttttaaggc
    23041 gatgttagag catttccttg tttttttgta aaatgttagg taaaataaaa ttaattaagt
    23101 aacactttct tagctaaagg aggagtgcat tcgatgaacg aagagacagt atcgccaatg
    23161 aagaagctta cgaagttagt tactcatact tcgtatcaac tttttgattc ttttgtaaat
    23221 gacactgaag aacatgctaa gaatgtacga ttgcaaggaa agtttattct tgagctgcaa
    23281 aaagctgcta ctgaacatag tctagttatc ttacaagtaa gagcaaatcg tttgagtagc
    23341 aagtatgaaa cgatctcagg ctgggtggcg acaaaaacgg tcgaaaaaga ccgagtgatg
    23401 atccgcctac aaaacgaaga gcagcagatc cggatggttc ctgtagaaca aattgaaaaa
    23461 ataacaatcc tttcaccagc aggggaacag gaacggttat caaaataatt tataccagat
    23521 agaaaagcac gtatgagctt gtaccatcaa tcattactaa tcaaaaataa ccgaatgctt
    23581 taagctgata tcagtaaaac ggaacgtttt aggatgagta tcatagcatg acagcattcg
    23641 gttgattagt tgaatcgatt ggatggtaca agtttttttg ataaaaaaag gtcctgctag
    23701 ccggggagct agcaggataa ggagttaaaa atgaaaaagt gttgttaggg ttgtttgttg
    23761 gtatgcttat ataatagtca ttagttgtga atattttgtg acgaaagttt tcgaaagttg
    23821 ttaagtt
//
LOCUS       DACGLE010000038        23571 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0534
            SAMEA3304449-rid9560453.denovo.039, whole genome shotgun sequence.
ACCESSION   DACGLE010000038 DACGLE010000000
VERSION     DACGLE010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304449
            Sequence Read Archive: ERR985064
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23571)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23571)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702225.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702225.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 20:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,045
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 27 of 67
            Pseudo Genes (incomplete)         :: 25 of 67
            Pseudo Genes (internal stop)      :: 19 of 67
            Pseudo Genes (multiple problems)  :: 4 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23571
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304449-rid9560453.denovo.039"
                     /strain="VREN0534"
                     /db_xref="taxon:1352"
     gene            complement(459..1088)
                     /gene="udk"
                     /locus_tag="IXT20_001629"
     CDS             complement(459..1088)
                     /gene="udk"
                     /locus_tag="IXT20_001629"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HAQ4860076.1"
                     /translation="MTKSKPIIIGVTGGSGSGKTSVSRAIFNNFPDHSIMMLEQDSYY
                     KDQSHLSFEERLNTNYDHPFAFDNDLLIQHVGDLLNYKAIEKPVYDYVAHTRSQATII
                     QEPKEVIILEGILILEDERLRDLMDIKVYVDTDDDIRIIRRIKRDMEERGRTLDSVIE
                     QYLTVVKPMYHQFIEPTKRYADIIVPEGGENHVAIDLITTKVASFLNHK"
     gene            complement(1172..1867)
                     /locus_tag="IXT20_001630"
     CDS             complement(1172..1867)
                     /locus_tag="IXT20_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="noncanonical pyrimidine nucleotidase, YjjG
                     family"
                     /protein_id="HAQ4860077.1"
                     /translation="MKYKTLLFDVDDTLLDFQLTEANALSALFAEQGITLTPEIETSY
                     KALNHHLWQEFEKGRLSREEIIGSRFGLLFQQFGKTVDSDQMEKRYRHYLNQGHDLVP
                     GSLEILKKVHPHADLYIVTNGVSHTQHRRLTDAGMLDYFKDIFVSEAVGAQKPMKEFF
                     DHVFSHIPNFDKKQTVIIGDSLTSDIKGGNMAGIDTIWFNRNKLPEIEEIQPTYRIDS
                     LEELYSLLEIEKD"
     gene            complement(1880..2596)
                     /locus_tag="IXT20_001631"
     CDS             complement(1880..2596)
                     /locus_tag="IXT20_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HAQ4860078.1"
                     /translation="MRLDKLIEEKLETTRKEMKRLFALKQVMIDGTVEFRQNRNVDSL
                     LHQIEVAGEHLETNESYYLLNKPEKAVTAVSDPEKTTVIDLIASADRFQPLYPVGRLD
                     RDTTGLLLITSNGQLGYEMLLPEKKVSKTYQVVVNERVTPADVTAFENGIVFADGYQC
                     KPADLKILKADKHQSTVLLTIQEGKFHQVKKMFLAQGKKVTALKRLSMGPLELDEQLG
                     VGEYRSLTLAEMKKLSIYFK"
     gene            2926..3531
                     /locus_tag="IXT20_001632"
     CDS             2926..3531
                     /locus_tag="IXT20_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HAQ4860079.1"
                     /translation="MSVSLEVSKREVRPRSLRNKLRHEGKVPAVVYGYKVESTPISFD
                     GLTLSKLLREHGANTVITMDIDGQKVNTLVHKIQTNTFTNHFEHAEFLSVNMSEETEV
                     ETEVVLVGESVGAKSGGVLAQNLYTVVVSATPDKLPERIEVDVTNLELGDSITVADLP
                     KNDDYKFVTDSEEQIAAVVAQSEEESTGEGGEPEVIGSTEE"
     gene            3856..4179
                     /locus_tag="IXT20_001633"
     CDS             3856..4179
                     /locus_tag="IXT20_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HAQ4860080.1"
                     /translation="MFESFDSSRNRYASVGVVSSLPDELIDSIWFIIDLDLKGVIPLE
                     NILTFDLIENRGKVTMHFSQAGSNVEMDIDLPFAYSNQFPAQVYAYDDGTRETILLPS
                     EIRQY"
     gene            4361..5731
                     /locus_tag="IXT20_001634"
     CDS             4361..5731
                     /locus_tag="IXT20_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HAQ4860081.1"
                     /translation="MRDLTKGSPAKLILMFTVPLLIGNVFQQFYNMIDMIIVGQTLGK
                     NALAAVGATGSLTFLIIGFAQGLTAGLAIITAQRYGAKDYRGLKKSFAASVVISLVVT
                     VILTVLSLLFIRPMLQLMQTPPEILDQAQTFISIILLGIFASVSFNLLSNVIRALGDS
                     RTPLFFLIIAVIINVVLDLIFIIYFGMGVEGAAIATVIAQVSSSVLCLVYIKKKMPLL
                     QLRKKDFKFEKEEFAVHLNAALPMAFQSSIIAVGAIVLQAALNSLGTDVVAAQAAAGR
                     IDQFANQPMMSFGIAMATFTAQNYGAKEYGRILQGVRQTLLMSVGFSLLAGAIVIFFG
                     HFFVQLFVSPSETRVFELAQTYFNINGSLYWILAVLFILRYTLQGLGQSKIPTIAGMM
                     ELLMRSFAAIVLTGMWGYPGAAAASPLAWIGSVAVLLYSYMRSMKQLKHMGEEEHFTL
                     EHAEAR"
     gene            complement(5861..7162)
                     /locus_tag="IXT20_001635"
     CDS             complement(5861..7162)
                     /locus_tag="IXT20_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HAQ4860082.1"
                     /translation="MVINFIILFLMIAITSFFVASEFALVKIRRSRLEQLEKENVKNA
                     KLALHVTHHLDNYLSASQLGITLTGLIIGWVGEGSVAALLEPVIGKLPFSSAISSTIS
                     VALGFILVTYIDVVIGELLPKSYSIVNTEKVVLFVVKPLHYFYKVMFPFIWVLNHSAA
                     GLGKLLGVRLVSEGEETLSQEELTLVALNSYEKGELTKEEYHYLENVFEFDDTLAKDI
                     QVDRTSMQVFEASETVKEAIKASIEQGHTRYPVILESKDNVLGYVTLPDLIKQSYKDD
                     QLTVEQLIEEPIVTTETIPIKKLLTIMRKKGKHIAILKDEYGGTSGLVTIEDILEEIV
                     GEIRDETDLDEALIAEQSDGSYIISGKLTLDDFQRYFHVEIPEFEETNFTTLAGFASS
                     RYKEIKAGTIIEIASFRFTVLEYQHAHIDYFKVESTERKIE"
     gene            complement(7392..9383)
                     /locus_tag="IXT20_001636"
     CDS             complement(7392..9383)
                     /locus_tag="IXT20_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter Kup"
                     /protein_id="HAQ4860083.1"
                     /translation="MATTHNTKKISMAGLLVAMGVVYGDIGTSPLYVMKSIVEDNGGL
                     SGINPDFVIGSVSLIFWTLTLLTTIKYVVIALNADNHGEGGIFSLYTLVRKGGKYLII
                     PAMIGGAALLADGVLTPAVTVTTAIEGLRGIPQFFDRFGNNQNVIVIITLVIIFVLFM
                     VQRFGTDIVGKAFGPIMFAWFTFLGVMGLMNFSQDWTVIRALNPYYAIQLLFSADNKL
                     GLFILGNIFLATTGAEALYSDLGHVGKRNIRLSWPYVKICLVINYFGQAAWLLNVYQN
                     PEAQQIKNLNPFFQMMPQSWIVIGVAFATVAAVIASQALITGSFTLVSEAIKLKLLPR
                     LKIMYPGNSIGQMYIPAVNLILWIACSLVVVTFRTSHHMEAAYGLSITVTMLMTTALL
                     YFYLIQNGHSRWLAYLVTFFFGAIEVVFFISSVVKFFRGGFVAVLIGLIILVVMFIWE
                     QGNIIRESSTEDVSLRDYIPQLRELRDDTSLPIYQTNVVFLVPDMAEDKVGRQFIYSI
                     LDKRPKRARVYWFVHVEVTDEPYTKEYQVDMMDTDFIVQVNLFLGFRVQQEINVYVRQ
                     VIHDLMKQGRLPKQPQRYSLTPGREVGDFQFVLIDEVVSNVTTLGKWQRQIMQAKLAI
                     KKIATSPETWFGLEYSEVKHETVPLIIGNTRKTWLKERL"
     gene            complement(9527..9793)
                     /locus_tag="IXT20_001637"
     CDS             complement(9527..9793)
                     /locus_tag="IXT20_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAQ4860084.1"
                     /translation="MLNFLWSLFIGGILGLIAGAILGKEVPGGVSGNIIIGFLGSWLG
                     EFLLGPLGPELGGFYLIPALLGAIFCLALYSFVARRIRERPKKE"
     gene            complement(10303..12429)
                     /gene="pnp"
                     /locus_tag="IXT20_001638"
     CDS             complement(10303..12429)
                     /gene="pnp"
                     /locus_tag="IXT20_001638"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HAQ4860085.1"
                     /translation="MSEKQVFKTTWGGRPLQIEVGQLAKQANGAVLVRYGDTVVLSAA
                     VASKEAKDTDFFPLTINYEEKMYAAGKIPGGFIKREGRPSTEATLTARLIDRPIRPMF
                     AEGFRNEVQVTNIVMSVETDCSPAMAAMLGSSLALSISDIPFDGPIAGVEVGRVNGEY
                     VLNPTVEQAEQTDIELTVAGTKQAINMVESGAKEVSEEDMLGALLFGFDAIKELVAFQ
                     EEIVQAVGKEKMEVTLLQVDEVLKKEIFDASYATMKAAVMTEEKLAREDNIEQVKIDI
                     REAYAEKFAGHEDEDHLLKEVKQITEDLEKDVVRELITIDKIRPDGRKLDEIRPLSSE
                     VSLLPRVHGSGLFTRGQTQALSACTLAPLGEHQIIDGLGVEVSKRFIHHYNFPQFSVG
                     STGRAGSPGRREIGHGALGERALAQVIPSEEEFPYTIRLVAEVLESNGSSSQASICAG
                     TLALMDAGVPIKAPVAGIAMGLVSDGENYTILTDIQGLEDHLGDMDFKVAGTKDGITA
                     LQMDIKIQGITEQILTEALTQAKQARMEILEELTSTIAAPREELSQYAPKIEMIQIEP
                     AKIKDVIGKGGDTINGIIDETGVKIDIDQDGKVSIASADAEMIKKAIKIIEDLTKEVK
                     VGEVYLGKVVRIEKFGAFVNLIKGKDGLVHISQLANDRVNKVEDVVKLGDEILVKVTE
                     IDKQGRVNLSRKAMLNEDGSTKNEEK"
     gene            complement(12617..12886)
                     /gene="rpsO"
                     /locus_tag="IXT20_001639"
     CDS             complement(12617..12886)
                     /gene="rpsO"
                     /locus_tag="IXT20_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HAQ4860086.1"
                     /translation="MAISKERKNEIINEYARHEGDTGSPEVQIAVLTEEINHLNEHAR
                     VHKKDHHSYRGLMKKVGHRRNLLAYLRKTDVQRYRELIKRLGLRR"
     gene            13137..13700
                     /locus_tag="IXT20_001640"
     CDS             13137..13700
                     /locus_tag="IXT20_001640"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HAQ4860087.1"
                     /translation="MITMDDIIREGNPTLREVAKEVSLPLSEEDISLGKEMLEFLKNS
                     QDPIKAEELHLRGGVGLAAPQLDISKRIIAVHVPSPDPEADGPSLSTVMYNPKILSHS
                     VQDACLGEGEGCLSVDREVPGYVVRHAKITVSYYDMNGEKHKIRLKNYESIVVQHEID
                     HINGVMFYDHINDQNPFALKEGVLVIE"
     gene            complement(13953..14474)
                     /locus_tag="IXT20_001641"
     CDS             complement(13953..14474)
                     /locus_tag="IXT20_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HAQ4860088.1"
                     /translation="MIEGETRRKQIMQTLANTEKPVSASKFAKTFGVSRQIIVGDIAL
                     LRAAGKSIVATARGYILETEETNTGHISKIAVQHGKDQTEEELRLIVENGGEIIDVIV
                     EHPLYGELAGTLHIKTPQDIDSFMRRYQKSNATLLSELTGGIHLHTIRYPEKHMLKNI
                     KQKLAEAGILYEG"
     gene            complement(14477..15319)
                     /locus_tag="IXT20_001642"
     CDS             complement(14477..15319)
                     /locus_tag="IXT20_001642"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HAQ4860089.1"
                     /translation="MTEFKTEKEKMIAGELYFANDPELVADRMFARSQSQIINQAESA
                     ELRSQLLKETFGRTGKKIYMEPVINFDYGYNIFVGENFYANFNCTFLDVSTIEIGDNC
                     MFAPNVQLYTATHPLHPVKRNSGLEYAKPIKIGNNVWLGGGVIVTPGVTLGDNVVVGA
                     GSVVTKSFPDNVVIAGNPARIIKTVEEELTEESLETLRHKIDMIDRELVALLEKRMDT
                     VTKIGQVKKEENQAVYDAKREQQVLDKVVSLLKNKGYKETITDTYVDLMKHSREYQNK
                     MKEE"
     gene            complement(15312..16037)
                     /locus_tag="IXT20_001643"
     CDS             complement(15312..16037)
                     /locus_tag="IXT20_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HAQ4860090.1"
                     /translation="MKQLTVDEAAKEILAYQQITFLTGAGVSTPSGVPDYRSLSGIYH
                     GMEQPEYLLSHQAMVNEPDKFYQFVKKIYHPEAKPNIIHLEIAHLAKEKNVWVVSQNI
                     DGLHAKAKSPQLVNFHGSLYQCHCRKCQQTVDWKEYLHSDKHQACGGQIRPDIVLYGE
                     GFQDGVMEQAAYAVSQAELIVIVGTSFQVHPFCDLVQFRQPSAKILVINQTPVYLAEP
                     YTFIQEDGAVVFKKIQELGADYD"
     gene            16213..16509
                     /locus_tag="IXT20_001644"
     CDS             16213..16509
                     /locus_tag="IXT20_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4860091.1"
                     /translation="MLPFFIYWGVILACIAWLALSVYFSVFYLVRKENGNLWAFALFN
                     VIAAIVLAITLAVYRTWGWGITQYSSLIYLILAIYGVVVLLQAILGREPKKAAA"
     gene            17077..18669
                     /locus_tag="IXT20_001645"
     CDS             17077..18669
                     /locus_tag="IXT20_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750719.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAQ4860092.1"
                     /translation="MKKNMVSVAVLFAFVFSLFSVGSLANADEKTPSDEFRVGMEAGY
                     APFNWSQQTDENNALPIQGQNSYAGGYDVQIAKKVADGLGKKLVIVQTKWDGLAPALQ
                     SGKIDAIIAGMSPTAERRKEIDFTDPYYESQLVVVVQKDSKYAKAKSLADLSGAKITA
                     QLNTFHYTVIDQIPDVQKQQAMDNFSAMRTALASGMIDGYVSERPEGVTATSVNKDLE
                     MLEFSGADGFQTNPEDVQISVGMRKGDPDIQKVNQILSGISNDERTKIMDQAVKDQPA
                     ATDSEEKPGIISDFTTIWKQYGNMFLRGAGLTLFIALIGTVVGTTLGLLIGVFRSVPE
                     SGNALTRFFQKIGNALLSIYVEVFRGTPMMVQAMVIFYGLALAFGISLNRTIAALFIV
                     SLNTGAYMTEIVRGGIFAVDEGQFEAAQAIGMTHGQTMRKVVIPQVLRNILPATGNEF
                     VINIKDTAVLSVIGVADLFFQGNSASGANFQFFQTFTIVGIIYLVMTFTITRILRAVE
                     KKIDGPSAYTKLEEIDNSNLQE"
     gene            18682..19422
                     /locus_tag="IXT20_001646"
     CDS             18682..19422
                     /locus_tag="IXT20_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HAQ4860093.1"
                     /translation="MNEKIIEIKHLRKSFGENEVLKDISVTVNKGEVVTIIGSSGSGK
                     STFLRCINLLEKPTGGEIIYNGENVLAPKYNLPKYRTNLGMVFQSFNLFNNMNVLENC
                     MSGQTTVLKRDKETAKKVAIENLEKVGMDRYIEAKPAQLSGGQKQRVAIARALSMNPD
                     VLLFDEPTSALDPEMVGEVLNTIKDLAHTGLTMLIVTHEMEFAKDVSDRVIFMDKGVI
                     AEEGTPEDIFVHPKEERTKEFLSRILNA"
     gene            19590..20939
                     /locus_tag="IXT20_001647"
     CDS             19590..20939
                     /locus_tag="IXT20_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ4860094.1"
                     /translation="MNEEQELQRGLKNRHVQLISIGGAIGTGLFLGSGKSIQLAGPSI
                     LLAYLITGCICFFIMRALGELLLSNTNNHSFLDFVAEYLGKKAAFITGWTYWFCWVSI
                     AMADLTAIGMYVRYWAPGVPQWLPELIALGLLLCLNMVAVSLFGELEFWFALIKVVAI
                     IAFILVGAYMILTHYTTDIGTASITNLWSHGGFFPTGAKGFILAFQMVTFAFAGIELV
                     GLVAGETENPEKVLPEAINNIPIRIILFYLGSLFVIMAIYPWNSLNPDNSPFVEVFSE
                     IGITIAASLINLVVLSAAASACNSAIYSTGRMLRSLAQEGSAPKKFKKLTTHHVPGNA
                     LTFSTIVIFISVILNYVMPSEVFTLVSSIATTCFLFIWSMLVYTHMKYRKSILGKKAH
                     SFKMPLYPFSNYLVFLYMAFVCVVLFLGKDTRIALLLTPVWFLLLLLIYHMKYERKK"
     gene            complement(21067..21657)
                     /locus_tag="IXT20_001648"
     CDS             complement(21067..21657)
                     /locus_tag="IXT20_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1211 domain-containing protein"
                     /protein_id="HAQ4860095.1"
                     /translation="MTKSRLEAFTDGVVAIVLRVIVLEIKIPNTSSFHSLWVIRNTLL
                     AYTISFIFVAVIWVNHHRIFQMAERINYRVVWSNIFLLFWLTLCPSVTSWVGRNPEAF
                     WPEMAYVLVYTMWSFSFGILMRQIMKANKPDSHVVRVLSKDRRSLISMMINLCLIAGV
                     FFVPLIGLFGRFFVSGIWIFSYKKADAYYQRLFPGK"
     gene            21804..23156
                     /gene="brnQ"
                     /locus_tag="IXT20_001649"
     CDS             21804..23156
                     /gene="brnQ"
                     /locus_tag="IXT20_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HAQ4860096.1"
                     /translation="MTKKLSFKDYLFIGSMLFGLFFGAGNLIFPVHLGQEAGAATFWA
                     NLGFLVTGIGLPFLGVIAIGVSKSSGVYELAKRINQTYAMVFTILLYLVIGPFFALPR
                     LATTSFEIGLAPFIDPSRQTLYLAIFSIVFFVLAWWFSRKPTKILDYVGKFLNPAFLV
                     LLGALLLLAFLNPLGSVNQAPIQPNYQEHAFFTGFTQGYNTLDALAALAFGIIIVTTI
                     QNMGVTKPAEIAKDTIKSGAISIILMGIIYTLLAYLGAMSLGSFALSENGGITLAQIA
                     DHYLGTYGSILLAFIVILACLKTGIGLITAFAETFTDLFPQRNYAFFVALASILPCLA
                     ANVGLTNIIQFSLPVLMFIYPLAMTLILLVLVGPLFKQRTAVYRMTTYFTLIASIFDG
                     LNACPETIKQTPIVQNLLYVAESYLPFFKLGMGWIVPAVIGFVIGLIWSFAKKEEVAD
                     "
BASE COUNT         6750 a         4746 c         4490 g         7585 t
ORIGIN      
        1 gggggtgcga caaaagagct tcttcaaaaa aataagtcga aatctcacac aaattcgaga
       61 aataattttc gtaagtttct tcttatttat tgcatctgac cacttctgtt acaacctctc
      121 gttaaacggt gttcaaaagc ttgcatctac gataataagc ccaacccaac gaaaaacgtg
      181 aggagctgtt ttatttggat gctcttatcg cttgatgcaa agacagcttt tttacaacct
      241 ctacttcacg gtgctcaatc agctgacctt cggtattagc tcaaaaaacg gtaaaatata
      301 aacagctatt ttccgtttct tctttttaat acttaggtca agatgctgtt ttcacaacct
      361 cttgttataa ataaaaaaac agttggctgg tgactgttca aaaagcagaa tccttgcttt
      421 tcaaacggtc gcagtccgtt caactgtttc gctgcgcact atttatggtt taagaaacta
      481 gctacttttg ttgtaatcaa atcaatagca acgtggtttt ctccgccttc tggaacaatg
      541 atatccgcat agcgcttcgt tggttcgata aattgatgat acatcggttt aacaactgtc
      601 aaatattgtt cgatcacgga gtccaatgtg cgtccccgtt cttccatatc ccgtttgata
      661 cgacgaatga tacgaatgtc atcatctgta tctacgtata ctttgatatc catcaagtca
      721 cgcagacgtt cgtcttctaa aatcaggatt ccttctagaa tgatcacttc ttttggttcc
      781 tgaatgatcg ttgcctgact tcttgtatgc gctacatagt catacacggg tttttcaatg
      841 gctttatagt tcagtaagtc gcctacatgc tgaatcagca gatcattatc aaaggcaaat
      901 gggtgatcgt aatttgtgtt caatcgttct tcaaaactca aatggctctg atccttgtaa
      961 taagaatctt gttcaagcat catgatggaa tggtccggga agttgttgaa aatcgcacgg
     1021 ctgacacttg tctttccgct tccggatcca cccgtcacac caatgataat tggcttgctt
     1081 tttgtcattg actaaaacct ctttcgtatc cgttttttca ttctaatcta ttatagcgaa
     1141 aaaacacgaa aatgctatat ttttctgaaa attaatcctt ttcaatttcc aataatgaat
     1201 aaagttcctc taatgaatcg attcgataag taggctggat ttcttcgatt tctggtaatt
     1261 tatttctatt gaaccaaatc gtatcgatcc ctgccatatt tcctcctttg atatcagaag
     1321 tcaaagaatc accaatgatc acagtttgct ttttatcaaa attaggaatg tgggaaaaga
     1381 catgatcaaa aaattctttc atcggctttt gcgctccgac agcttctgag acaaagatat
     1441 ccttgaagta atcaagcatt cctgcatctg ttaatcgccg atgttgcgtg tgcgacacac
     1501 cgttcgtcac aatataaaga tcagcatgcg gatggacctt tttcaggatt tctagacttc
     1561 ctggaacaag atcatgacct tggttcaagt agtggcggta gcgtttttcc atttgatcac
     1621 tatctactgt tttcccaaat tgctggaaaa gtaacccaaa acgactgcct attatttctt
     1681 ctcgtgatag tctgcctttt tcaaactcct gccataaatg atgattcaaa gccttatagc
     1741 tggtttcgat ttctggtgtc aatgtgattc cttgttctgc aaacaaagca cttaaagcat
     1801 tggcttcagt taattgaaaa tctaacaatg tatcatctac atcgaacaat aaagttttat
     1861 atttcatctt tttgacctcc tatttaaaat aaatacttaa ctttttcatc tctgctaatg
     1921 tcaacgatcg atactcgcct actcctagct gttcatctaa ttccaatggt cccatgctta
     1981 gccgtttcaa tgcggtcact ttctttcctt gggctaagaa catctttttg acttgatgga
     2041 attttccttc ttggatcgtc aataaaaccg tactttggtg cttgtctgct ttgagtattt
     2101 ttaaatcagc cggcttacat tgatacccat cagcgaatac gattccattt tcaaatgctg
     2161 ttacgtctgc cggtgtcacc ctttcgttca caactacttg ataagtcttt gaaacctttt
     2221 tttctggaag tagcatttca tatcccaatt gtccattcga agtgatcagc aatagtcctg
     2281 ttgtatctct atctagtctt cccactggat ataatggttg gaaacgatcg gcacttgcaa
     2341 tcagatcgat tactgtcgtc ttttctggat ctgatacagc tgtaacagct ttttcaggtt
     2401 tattgagaag ataataggac tcatttgttt ctaagtgttc acctgctact tcgatttgat
     2461 gcagtaaact atctacattt cgattttgtc gaaattcgac tgtgccatct atcatcactt
     2521 gcttcaaagc aaacagccgc ttcatttcct tgcgtgtcgt ttctaatttt tcttctatta
     2581 atttatctaa acgcatgctg tttcctttct tattactact aattaattat accttgaata
     2641 ttcttgcttg caaatacatc atcatagtta ttccttgact taattaacag ttcacgttac
     2701 ggttaaatag taaagtgaat tggatttctc atacacagcg taatgacctg gtggagaaaa
     2761 gttgaatcct gactttctaa ggggagatgc gcgcatctct ccttttgcgc ttttttaagc
     2821 gccctgcctt cgatgatgtg cacgagatta gcctttagaa ttcacttaaa taaaatctgt
     2881 ccgttaccaa agcagacagt cacattttaa ggaggaattt tggttatgtc agtatcatta
     2941 gaagtaagta aaagagaagt acgtccccgt tcattaagaa acaaacttcg tcacgaaggg
     3001 aaagttccag ctgttgttta tgggtataaa gtagaaagta cccctatctc attcgatggt
     3061 ttgacactaa gcaaattgtt gcgtgaacat ggagcgaaca cagtcatcac gatggacatc
     3121 gatggacaaa aagtcaatac attggttcac aagatccaaa cgaacacttt taccaatcat
     3181 ttcgaacatg cagaatttct atctgtaaac atgtcggaag aaacagaagt cgaaacagaa
     3241 gtcgttcttg ttggcgaatc agtgggtgct aaatctggtg gagtgttagc tcaaaacttg
     3301 tatacagtag tcgtatctgc tacaccagac aaattgcctg aacgaattga agttgacgtg
     3361 acaaacttgg aattaggtga ttctattaca gtagccgatc ttccaaaaaa cgacgattac
     3421 aaattcgtga ccgacagtga agagcaaatc gctgctgtag tcgctcaaag tgaagaagaa
     3481 agcacaggtg aaggtggcga accagaagtg atcggttcaa cagaagaata atactgaaac
     3541 aatcaggtgg tgttagaagc ggcgggtcag cagcttttaa cacgcctttt ttatttctaa
     3601 tcgtttattt tcatatgggg cttccacttt tgtaaatggc tttacaaacg aaaaatccta
     3661 atttaccaag cttttcctcg catactttta tcgtaccttt ataaaaattt atactttgaa
     3721 aaaaagtatt caattattgt tgcatttttc ctgattcttt attagaatga accctcgttg
     3781 aagtaagtat ttctagttta tcctaggatt aattataaaa ataaaacatt cgttgattcg
     3841 aaggaggtta aacagatgtt tgaaagtttt gatagcagcc gcaaccgtta cgcttcagtt
     3901 ggtgtcgtat caagtttgcc agatgaattg attgacagta tatggtttat tattgaccta
     3961 gatcttaagg gagtcattcc attagaaaat atattaacct ttgatttgat cgaaaatcgt
     4021 ggaaaagtga cgatgcattt ttctcaagca ggaagcaatg tggaaatgga tattgatttg
     4081 ccgtttgcct attcgaacca atttcctgct caagtatacg cctatgatga cggtacgaga
     4141 gaaacgatcc ttttaccaag tgagattaga caatattaag gtgatagata aacgtggatg
     4201 tcccttattt tacagaccag gccaagttgt tgattctttg actcaattct tggcttcttt
     4261 ttatggagtt tcataaatta tcattgattt ctaattggta ttctcaggat aaacgctata
     4321 tactactaac taaattttaa agatataagg agtttaaaag atgcgcgatt taacaaaagg
     4381 aagccccgct aaactgattt tgatgttcac tgttccttta ctgatcggaa atgtttttca
     4441 gcagttctac aatatgatcg atatgatcat cgtgggtcag acgctaggta aaaatgcgct
     4501 ggcagcggtt ggtgcaactg gaagcctgac atttttgatc attggctttg cccaaggttt
     4561 aacagctggt ttagccatta ttaccgcaca acgttacggt gcaaaagatt atcggggtct
     4621 gaagaaaagt tttgctgcta gtgttgtcat tagtcttgtc gtcactgtga ttttaactgt
     4681 cctaagtctc ctgtttattc gtccgatgct tcaattgatg caaactcctc ctgaaatact
     4741 tgatcaagca caaacattta tttcaattat tttattaggg atctttgcat cagtcagttt
     4801 caatttgctt tctaatgtca ttcgcgcact tggtgacagc cgcacaccac tattctttct
     4861 gatcattgcg gtcatcatca acgtcgtact tgatttgatc tttattatct attttggcat
     4921 gggtgtggaa ggtgcagcta ttgctacagt aatcgcacaa gtttcttcca gcgtactgtg
     4981 tctagtgtat atcaagaaaa aaatgccttt gttacagtta cgcaaaaaag atttcaaatt
     5041 tgaaaaagaa gagtttgctg tacacctaaa tgctgctttg ccaatggcgt ttcaatcttc
     5101 gatcatcgct gttggtgcta tcgtgttaca agcagcacta aatagtctag ggacagatgt
     5161 cgtagctgct caagccgctg ctggtcgtat cgaccaattt gcaaatcagc cgatgatgtc
     5221 atttggtatt gcaatggcaa ctttcactgc acaaaactat ggtgcgaaag aatatggccg
     5281 aatcttgcaa ggggtacgtc aaactttact gatgagtgtc ggatttagtc tcttagcagg
     5341 tgctatcgtc attttcttcg gtcatttctt tgttcaattg tttgttagcc catcagaaac
     5401 acgtgtattt gaattagcac aaacctactt caatatcaat gggtctcttt actggatctt
     5461 agcagtcctg ttcattttac gctacacact tcaagggctt ggacaaagca agatccctac
     5521 gatcgccggt atgatggaat tgcttatgcg ttcttttgca gctatcgttc ttacaggaat
     5581 gtggggatac ccaggagcag cagcagcttc tcctttagct tggatcggtt ctgttgccgt
     5641 gttgctttac tcttatatgc gttcgatgaa acaattgaaa cacatgggtg aagaggaaca
     5701 tttcacatta gaacatgcag aagcaagata gccttgatta aaagaaatcg ttgcggctaa
     5761 aaatgaaaac ttttattgaa aatatcttct tcgtttaccg aaaacaggtt ttttagataa
     5821 caaaaaggct gtgacaaaaa atgtcacagc cttttttctt ttattctatt tttctttccg
     5881 tggactctac tttgaaataa tcgatatgtg catgctggta ttctaatacc gtgaaacgga
     5941 atgatgcaat ctcaatgatt gttcctgctt ttatttcttt atatcggcta gaagcaaagc
     6001 ccgcaagtgt agtaaaattc gtttcttcaa actctggaat ctctacatga aaataacgct
     6061 ggaaatcatc taatgttagt ttgccggaga tgatataaga gccatctgac tgttccgcta
     6121 tcaatgcttc gtccaaatct gtctcatctc ggatttcccc gacgatttct tctaaaatat
     6181 cttcaatcgt cactaagccg cttgtccctc cgtattcatc ttttaagata gcaatatgct
     6241 ttcccttttt tcgcatgatc gtcaataatt tttttatagg gatcgtttca gttgttacga
     6301 tcggctcttc aatcaactgt tctactgtca gctggtcatc tttataagat tgcttgatca
     6361 aatctggcaa ggtgacataa cctaaaacat tgtcttttga ttctaagata accggatagc
     6421 gagtatgtcc ttgttcaatc gaagctttta tcgcttcttt gacggtttct gaagcttcga
     6481 atacttgcat ggacgtacgg tctacttgta tatcttttgc caaggtatca tcaaattcaa
     6541 acacgttttc caaataatga tattcttctt tggtcaactc tcctttttca taagaattca
     6601 atgcgactaa tgtcagctct tcttgagaca atgtctcttc tccttcagat acgagacgca
     6661 cacctaatag cttccctagc cctgcagctg aatgattcaa tacccaaata aaaggaaaca
     6721 ttactttata aaaataatga agtggtttaa ctacgaacaa gacgaccttc tccgtattga
     6781 cgatactata gctcttagga agcagttcgc caataaccac atcgatatac gtaactaaaa
     6841 taaatcccag cgccacagag attgtactgc taatagccga actaaaaggt agtttaccga
     6901 tcactggttc tagtaaagcc gcgacagatc cttcaccgac ccacccgatg atcaaaccgg
     6961 ttagcgtaat tcctaattga ctagctgata aatagttatc taaatggtgt gtaacatgaa
     7021 gagctagctt ggcgttcttt acattttcct tttctagttg ttccaatctt gatcgtctga
     7081 ttttcactaa agcaaattca cttgctacaa aaaatgaagt aattgcaatc atcaaaaata
     7141 aaataatgaa attgataacc atattctccc gcctttctct ttttctattg tacccttttt
     7201 ctacaaaaag attgaaaata atgcgtattc atatttagga ataaaaaagg agatgcggca
     7261 tttttcagcc acatctcctt atcacttcaa aatcattgcg ttacttgttg ctaaacagct
     7321 ttttcatgac ctctgataaa cggtgttcaa gtagctgaca ttcgacatta gttcaggtca
     7381 aagcgctact ttcacaaccg ctctttcaac catgttttcc gagtatttcc aatgataagc
     7441 ggaacagttt catgttttac ttcgctgtac tctagcccaa accatgtttc tggcgaggta
     7501 gcgatttttt tgattgccag ttttgcctgc atgatttgac gttgccattt tcctagagtt
     7561 gtcacattgg aaactacttc atcaatcagg acaaattgga aatcgccaac ttctcgccct
     7621 ggtgtcagag agtatctttg tggctgtttt ggcaaacggc cttgtttcat caaatcatgg
     7681 atgacttggc gaacatatac gttgatttcc tgttggaccc tgaaacctaa gaacagattc
     7741 acttggacaa taaaatcagt gtccatcata tctacttgat attctttcgt atatggctca
     7801 tccgtcactt ccacatgaac aaaccaatag actcgtgctc ttttaggccg tttatctaaa
     7861 atcgaataga tgaactgtcg gccaacttta tcttcagcca tatcaggaac caagaagacc
     7921 acatttgtct gatagattgg taaagaagta tcatcacgta attctctaag ctgcgggata
     7981 taatctctca aagacacatc ctcggttgac gattcgcgaa taatgttccc ttgttcccaa
     8041 ataaacatca cgactaagat gatcaaacca atcagaacgg ctacaaaacc gcctcggaag
     8101 aatttgacaa cacttgagat aaagaaaacg acttctatcg caccgaagaa aaaggtaact
     8161 aaatatgcaa gccaacgaga atgcccattt tggataagat aaaagtaaag cagtgctgtt
     8221 gtcatcaaca tcgtcacagt gatcgataga ccgtaagcag cttccatatg atgagacgtt
     8281 cggaatgtga caaccactag agaacaggcg atccataaaa tcagattgac tgctgggata
     8341 tacatttgcc cgatactgtt cccaggatac atgatcttta accgaggcag cagtttcaac
     8401 ttgattgctt ctgaaactag cgtaaacgaa cctgtaatca atgcttgcga agcaatgact
     8461 gcagcgactg ttgcaaaagc aacgccgatc acgatccaac tttgaggcat catttggaag
     8521 aatggattca ggttcttgat ttgctgtgct tcagggtttt gataaacatt caacaaccac
     8581 gcagcctgcc caaaataatt gataaccaaa caaattttga cgtaaggcca gctcagacga
     8641 atatttcgtt tgccgacatg tccaagatcg gaataaagcg cctctgcacc tgtcgtagcc
     8701 aggaaaatat ttccaagaat gaacaagcct aatttgttat ctgcactaaa aagcagctgg
     8761 atagcataat aaggattcag tgcacggata actgtccagt cttgactgaa gttcattaat
     8821 cccattacgc ctaaaaaagt aaaccaagcg aacatgattg gtccgaatgc ttttccgaca
     8881 atatctgtac caaagcgttg gaccatgaat aaaacaaaaa taataactaa agtgataatg
     8941 acgatcacgt tctggttatt accgaaccgg tcaaaaaact gcggaattcc tcgcaagcct
     9001 tcaatcgcag tcgttaccgt cacagcaggg gtcaataccc catctgctag taaggcagct
     9061 cctccgatca ttgcaggaat gatcaaatat ttcccacctt ttcggacaag tgtgtagagt
     9121 gagaaaattc caccttcacc atggttatct gcattcaatg caatcacgac atatttgatt
     9181 gttgtcagta gagtcaatgt ccaaaaaatc aatgagactg atccgataac aaaatcagga
     9241 ttgattcctg acaatccgcc attatcttca acaatcgatt tcataacgta aagcggactt
     9301 gtcccgatgt ctccgtagac gactcccatc gctacaagca ggccagccat tgatatcttt
     9361 ttggtattgt gcgttgttgc cacgtttttc cctcatttct tgttatatgg atatccttta
     9421 ggtaagcttg aatgatagat gatattctta tccatgacac ccgctttcct aaattgcttc
     9481 taattatcca agtaacacac tgaagtgtca acttatttga ataaaattat tctttctttg
     9541 gacgttctct gatacgtctt gccacaaatg aataaagagc aaggcaaaaa attgcaccca
     9601 ataatgctgg tatcaaataa aaaccgccga gttccggtcc cagtggtcct agtaaaaatt
     9661 cgccgagcca actacctaaa aaaccgataa tgatatttcc agatacgcct cccgggactt
     9721 cctttcctaa aattgcgcca gctatcaaac ctaatatacc gccgataaat aacgaccata
     9781 aaaaattcag catctccttt ccctccttta tccatatttc tcacttttat tttacgcttt
     9841 attcagaagg actccaaaaa tatgcgccaa gaaactccag aaaaaaagaa gttgtaacaa
     9901 aagtgttcag cttatttccg tagaagctgc ttctggaaca ccgtgaatca gaggttgtga
     9961 aatcagcggt ttgacctgag tattagagaa acaaacggaa aaatagttcc tcatattttg
    10021 ccgttttttg agctaatatc gagggacagc tgattgaaca tcgtttattg gagaggttgt
    10081 aaaaaagctg ttctgcatca agtaataaga acagccaagt aaaaacagct cctaatgttt
    10141 ttaacttggc tggacttatt accgcagatg caagcttttg aacaccgttt attcgaaaaa
    10201 aaaggactgt gacacggctt ttgtcacagt cccctttttt cgaataatta ttgtccccct
    10261 gtcaccttca cgatcacaag tacattattc ctatgaatta aattattttt cttcattttt
    10321 cgttgaacca tcttcgttca acattgcttt tcttgacaga tttacgcgtc cttgtttatc
    10381 gatttcagtt actttcacaa ggatctcatc acctagttta acaacatctt cgactttatt
    10441 gacacgatcg tttgctaatt gggaaatatg gacaagtcca tcttttcctt tgatcaagtt
    10501 gacgaatgca ccgaattttt cgatacggac aactttccct agataaactt caccaacttt
    10561 gacttctttg gtcaaatctt cgatgatctt gatagctttt ttgatcattt ctgcatcagc
    10621 tgaagcaata cttacttttc catcttgatc gatatcgatt ttcacaccag tttcatcgat
    10681 gattccgttg atcgtatcgc cgcctttacc gataacatct ttgattttag caggttcgat
    10741 ctggatcatt tcgatttttg gtgcatattg gcttaattct tcacgtggag cagcaatcgt
    10801 tgaagtcaat tcttctaaga tttccatacg tgcttgtttt gcttgagtta atgcttcagt
    10861 taggatttgt tctgtgattc cttgaatctt gatatccatc tgtaaagcag tgataccatc
    10921 ttttgtaccg gcaactttaa agtccatatc gccaagatga tcttccagcc cttggatatc
    10981 agttaggatc gtatagtttt caccatcaga aacaagtccc atcgcaatac cggcgactgg
    11041 cgctttgatt ggtacacctg catccatcaa tgctaatgtg ccagcacaga tacttgcttg
    11101 ggaagaagaa ccatttgatt ccaatacttc tgctactaaa cggatcgtat aaggaaattc
    11161 ttcttctgaa gggattactt gtgctaacgc acgttcacct aatgcgccat gtccgatttc
    11221 ccgacgccct ggagagcctg cacgtcctgt agaaccaaca gaaaattgag ggaaattgta
    11281 gtggtggatg aatcgtttgc tgacttccac ccctaaacca tcaatgattt ggtgctctcc
    11341 tagaggtgcg agtgtacaag ctgataaagc ttgtgtttgt cctcgtgtga acaaacctga
    11401 tccatgtaca cgtggcaata agctgacctc tgatgacaat ggacggattt catccaattt
    11461 acgcccgtca ggacggatct tatcgatggt gatcaattca cgtacgacat ctttttcaag
    11521 atcttctgtg atttgtttaa cttctttcaa caagtgatct tcatcttcat gacctgcaaa
    11581 tttctcagca taagcttcgc ggatatcgat cttcacctgc tcgatattat cttcgcgagc
    11641 aagtttttct tcagtcatta ctgctgcttt cattgtagcg taagaagcat caaagatttc
    11701 ttttttcaat acttcatcaa cttgcagtaa agtcacttcc attttttctt tccctactgc
    11761 ttgaacgatt tcttcttgga aagcaactaa ctctttgata gcgtcaaaac caaacagcaa
    11821 tgcaccaagc atgtcttctt ctgaaacttc tttcgcgccg ctttcaacca tattgatggc
    11881 ttgtttcgtt ccggcaacag tcaattcgat atctgtttgt tctgcttgtt ctactgttgg
    11941 attcaagacg tactcgccat ttacacgacc aacttctacg cctgcgattg ggccgtcaaa
    12001 tgggatatct gagattgata gtgctaaaga agaacctagc attgctgcca tagctggcga
    12061 gcagtctgtt tctacactca tcacgatgtt cgttacttgg acttcattac ggaagccttc
    12121 tgcaaacatc gggcgaattg gacggtcgat caaacgggca gtcaatgttg cttccgtact
    12181 tggacggcct tctcgtttga tgaaacctcc agggattttc cctgctgcat acattttttc
    12241 ttcataattg atcgttaatg ggaaaaagtc tgtatctttt gcttctttag aggccacagc
    12301 tgcacttaag actactgtat ctccataacg gactaaaacg gctccgtttg cttgttttgc
    12361 tagctgaccg acttcgattt gtaagggacg gccgccccat gtcgttttga atacttgttt
    12421 ttctgacata acttctcctt ttccttctcg tgaaagcaag acgctactaa acaaatgaaa
    12481 actgccgaaa gccgtctttt gagcagcttt catttggtta gtaggagtcc atctagcttg
    12541 atcacggaat tttacttaat tgatatacga gtaaaaaaag tgagattcat gtgaatccca
    12601 ctctttttga ctaaaattaa cgacgcaagc ctaaacgttt gatcaattcg cggtaacgtt
    12661 gaacgtcagt tttacgtaag taagctaaca agttacggcg gtgaccaact tttttcatta
    12721 ggccgcggta tgaatgatgg tcttttttgt gcacgcgtgc atgttcattc aagtggttga
    12781 tttcttcagt taatacagcg atttgtactt ctggagaacc agtatcccct tcatggcgag
    12841 cgtattcgtt gatgatttca ttcttgcgtt cttttgaaat tgccatttct ttcacctctt
    12901 tcgattattc ccctgcgact gagtaaaacg ttggtgattc gcttaaccaa gtggcaggtc
    12961 atgtgcctag agcacacagc tatactttac ctgaaaccgg gttgaaaatc aagtagttaa
    13021 tcattaagaa aaggtagttt tcaaacaatc ttactactac ttttccattt ttgtggtaaa
    13081 ctataatgcg aaaatagact tgaaacacag tcttttaaga aaggggtaca tattccatga
    13141 ttacaatgga tgatatcatt cgtgaaggaa atccgacctt acgcgaagtt gcaaaagaag
    13201 tttctcttcc attaagcgaa gaagatatct ctttaggaaa agagatgctt gagttcttaa
    13261 aaaacagcca agatccaatc aaagctgaag aactacactt acgaggcggt gttggcttag
    13321 ctgctcctca attagatatt tctaaaagaa tcattgctgt tcacgtacca agtcctgacc
    13381 cagaggcaga tggaccttct ctaagtactg ttatgtataa tcctaaaatt ttgagccatt
    13441 ctgttcaaga tgcttgttta ggggaaggtg aaggctgttt atctgttgat cgagaagtac
    13501 caggatatgt tgttcgccat gccaaaatca ctgtgtctta ttatgatatg aatggtgaaa
    13561 aacacaagat tcgtttgaag aattatgaat cgatcgtcgt tcaacatgaa atcgaccata
    13621 tcaacggtgt gatgttctat gaccacatca acgatcaaaa tccatttgca ctaaaagaag
    13681 gcgtattggt gatcgagtag agaattggca ggttccttcg atactatgct acaagaaagc
    13741 tttataaaaa aggggagtgc tcaaaagctg cacttccctt ttttatgttt ctccactaat
    13801 cgaaatttag atagacatct agaaatcgca attccaaaaa attctgtgta ttggatacgc
    13861 atcttttctt attttttctt atttcttcca tatgggtgtt tatctcagag atgatagaat
    13921 agtttctagg ataaaaagta gcagctacga tgttatcctt cgtagaggat tcctgcttca
    13981 gctagttttt gtttgatgtt tttcagcata tgcttttctg ggtagcggat cgtatgcaga
    14041 tggatgccgc ctgttaattc ggataacaaa gtagcattac ttttttgata acgtctcata
    14101 aaggaatcga tatcttgtgg cgttttgatg tgcaaagttc ctgctaactc accgtaaagg
    14161 ggatgttcca caattacatc aatgatttca ccaccatttt caacgattag tctcagttct
    14221 tcttctgttt gatcttttcc atgctggaca gcgattttac tgatgtgccc agtattggtt
    14281 tcttctgttt cgaggatata acctcttgct gttgccacga tacttttacc tgcagctcgt
    14341 agtaaagcaa tatcgcctac aatgatctga cggctgactc caaaagtttt cgcaaacttg
    14401 ctagcactca ctggtttttc tgtatttgcc aaggtttgca tgatttgttt tcgccgtgtc
    14461 tctccttcga tcacacttat tcctccttca ttttgttttg gtattcgcgc gagtgtttca
    14521 tcaaatcaac atacgtgtct gttattgttt ccttatatcc cttatttttc agcagactaa
    14581 caactttatc caatacttgc tgctctcttt ttgcatcata aactgcttgg ttttcttctt
    14641 ttttcacttg tcctatttta gttaccgtat ccattctttt ttctaataaa gcaaccaact
    14701 ctcgatcgat catatcgatc ttatgacgta atgtttcaag agactcttct gttagttctt
    14761 cctctactgt cttgatgata cgagcaggat ttcctgcaat cactacattg tcgggaaaag
    14821 atttggtcac aaccgaacct gcgccgacta ctacgttgtc ccctagagtg acacccggcg
    14881 tgacgataac tcctccgcct aaccaaacat tattaccgat tttaatgggt tttgcgtact
    14941 ccagaccgct gttacgtttc actggatgta gtggatgcgt cgccgtgtaa agctggacat
    15001 tcggagcgaa catgcagtta tcaccgattt caatcgtgct gacatctaaa aatgtacaat
    15061 tgaaatttgc ataaaaattc tccccaacaa atatattata tccgtaatca aaattgataa
    15121 ctggctccat atagattttt ttccctgtac ggccaaaagt ctctttcaac aattgggatc
    15181 gcagttccgc tgattctgct tggttgatga tttggctttg acttcttgca aacatacggt
    15241 cggcaaccag ctcaggatca ttggcaaaat aaagctcgcc tgcaatcatt ttttcttttt
    15301 cggttttgaa ttcagtcata atctgctcct aattcttgta tttttttgaa aacaaccgct
    15361 ccgtcttctt gaataaacgt ataaggctcc gctaaataaa caggcgtttg attgattact
    15421 aagattttgg ctgacggctg gcgaaactgt accagatcac aaaacggatg aacttgaaaa
    15481 ctcgttccaa caatgacgat cagctctgct tggctgacgg cataagccgc ctgctccatg
    15541 actccatctt gaaacccttc accatataac acgatgtctg ggcgtatttg tcctccgcat
    15601 gcttgatgtt tgtcgctgtg aagatattct ttccagtcta ccgtttgttg acattttcga
    15661 caatgacatt gatacagact cccatgaaaa ttgacgagtt gcgggctttt agcctttgcg
    15721 tgcaaaccgt cgatgttttg ggaaacaacc caaacatttt tttctttcgc tagatgtgct
    15781 atttccaaat gaatgatatt gggttttgct tcaggatgat aaatcttttt gacaaattga
    15841 tagaatttat ccggttcatt caccattgct tgatgactca gcaagtattc cggctgctcc
    15901 atcccgtggt agattcctga caaagatcga tagtctggca ctccagaagg tgtggatacc
    15961 cctgcaccgg tcaaaaatgt aatttgctga taagctaata tctcttttgc tgcttcgtct
    16021 actgttaatt gtttcatgtt ttgtcacctc actttccatt atagcccata ctcgaacaaa
    16081 tttattgttg aaaagtcaga aaaatgggaa taaaaaagtg ttatgggcat ttgagcaatt
    16141 tatcacaatg ttcactgaca aaagaatgaa aacgtgttac aatactgctg tataaaagtg
    16201 aggtgaatcg atatgttacc attctttatt tattggggcg ttattctggc ttgtatcgcc
    16261 tggttagcgt taagcgttta tttctctgtt ttttatcttg tacgcaaaga aaatggaaat
    16321 ctttgggctt ttgctttatt caacgtgatt gccgcaattg ttttagccat tacattagcc
    16381 gtatatcgaa catggggctg gggaatcacc caatactcaa gtttgattta tctaatatta
    16441 gccatttatg gcgtcgttgt tcttttgcaa gcgatcctag gacgtgagcc aaagaaagcg
    16501 gcagcttaaa tgatcgagat ggaactggac acaggaaatt ttctgttcta gttctttttt
    16561 tattgtatta catacatttt tgtgtataat tatactatcg tttgcaatgg atagatgatt
    16621 cacattttga ttgttttttc ggtaaaagta aagatttcta tatttatcag tattttttca
    16681 aaaaccagca gttatagatt gacaaagcct ctcgagtatc gtaaactttt tttgtataaa
    16741 taactgaata cgagatgcct caatcaatga ggtagaggtc gcgagaatca ttaaccctgt
    16801 ggagtgaagc aaacatggag aagcaggaat agggaggatc gccgaaatgt gcactttttt
    16861 gcttggagtg tatgttggga cgcaagttaa gagcttgcgg actgtcttag cagtgatgct
    16921 aagttgcgct atgttttgtg agaggtttat caccttatgg aattttagcg gcctttttat
    16981 atctataaat cggtcgcttt ttattttttc agataaagct ttaccgcatt tcatctagac
    17041 cgctcgtgta tcatataatc agaaagggag aaaaaaatga aaaaaaatat ggtttcagtt
    17101 gccgtactat ttgcttttgt cttctcacta ttcagtgtgg gatctctggc aaatgcagat
    17161 gaaaaaacac cgagcgatga gtttcgcgta ggtatggagg ctgggtatgc acccttcaac
    17221 tggtctcaac aaacagatga gaataatgcc ttgccaattc aaggccaaaa tagttacgcc
    17281 ggcggttatg atgttcaaat cgctaaaaaa gtagcagatg gtttagggaa aaaattagtc
    17341 atcgtccaaa cgaaatggga tggtctggct cctgcccttc aatcaggcaa gatcgacgcg
    17401 atcatcgccg gaatgagccc tactgctgaa cgacgaaaag aaattgattt taccgatcct
    17461 tattacgaat cacaattagt agtagtagtt caaaaagata gcaaatatgc aaaagcaaaa
    17521 agtttagcag atctttctgg agcaaagatc actgctcagt taaatacatt ccactatact
    17581 gtgatcgatc agataccaga tgtgcagaaa caacaagcaa tggataattt ctcagcaatg
    17641 agaacagctt tagcttctgg aatgatcgat ggttatgtca gcgaacgtcc ggaaggtgta
    17701 acggcaacta gtgtgaacaa agacttggaa atgcttgaat tcagcggtgc agatggcttc
    17761 cagacaaacc cggaagatgt tcagatttct gttggtatgc gtaaaggcga cccggatatc
    17821 caaaaagtaa accagattct ttctggcatc tctaacgatg aacgaaccaa aattatggac
    17881 caagcagtca aagatcagcc tgctgcaact gatagtgaag aaaaacctgg catcatttct
    17941 gatttcacta cgatttggaa acaatacggc aatatgttct tacgaggtgc tggacttacc
    18001 ttgttcatcg cattgatcgg gacagttgtc ggtactacac ttggattact gattggtgtc
    18061 ttccgttcgg tacctgaatc aggaaatgca ctgactcgct tcttccaaaa aatcggaaat
    18121 gcacttcttt ctatctatgt cgaagtattc cgcggaacac cgatgatggt acaagcaatg
    18181 gtcatcttct acggtctggc actcgctttt ggcatttcat tgaaccgaac gattgccgct
    18241 ttgtttattg tctctctcaa tactggagct tacatgacag agatcgttcg tggggggatt
    18301 tttgcagtag atgaagggca gttcgaagct gcgcaagcca ttggtatgac ccatggccaa
    18361 acgatgagaa aagttgttat tccacaagtt ttacggaata tcttgccagc aacaggaaat
    18421 gagtttgtta tcaatatcaa agatacagca gttttaagtg taatcggcgt agctgatttg
    18481 ttcttccaag gaaactctgc ttccggtgcg aatttccaat tcttccaaac atttacgatc
    18541 gtcgggatca tctacttagt catgaccttt acgatcacca gaatcctgcg ggcggtagaa
    18601 aagaaaatcg atggtccttc agcttatacc aagcttgaag aaatcgacaa ttcaaatttg
    18661 caggaataag gaggaagaat catgaatgaa aaaattattg aaatcaaaca tctaagaaaa
    18721 agcttcggtg aaaatgaagt attgaaagat atcagcgtaa cagtcaataa aggtgaagtt
    18781 gtgacgatca tcggttcttc cggttctggt aaatctactt tcttgcgctg tatcaattta
    18841 ctggaaaaac caacaggcgg cgagatcatt tataatggag aaaatgtatt agctcctaaa
    18901 tataatttac ctaaatatcg aacgaatcta ggtatggttt tccaatcctt caacctgttc
    18961 aacaatatga atgtcttgga aaactgtatg tctggtcaaa caactgtcct gaaacgagat
    19021 aaagaaactg ccaaaaaagt agcaatcgaa aatctggaaa aagttggtat ggatcgttat
    19081 atcgaagcca aaccagcaca attatctggt ggacagaaac agcgtgtcgc aattgctcgg
    19141 gcattatcaa tgaatccaga tgtattgcta ttcgacgaac ctacttcggc gttagaccct
    19201 gaaatggttg gcgaagtgct taatacgatc aaagacttag cacatactgg cttgacaatg
    19261 cttatcgtga cgcatgagat ggaatttgca aaagatgtat ctgaccgtgt catttttatg
    19321 gataaaggtg tgattgcaga agaaggtaca ccagaagata tctttgtcca tccgaaagaa
    19381 gaacgtacaa aagaattcct ttcacgtatt ttaaatgctt aggatcaata gctgattggg
    19441 acaaagagct gcggtgttta ccgcagttct tttttatgaa ttactcataa aaggtaaatt
    19501 tctgaaaatt ttggaatctt agttgtgttt ttcagaaaac tctcttaata tggaagtaat
    19561 taaacttttt tagttaggat gatttaaaaa tgaatgaaga gcaagaactg cagcgtggac
    19621 tgaaaaatag acatgtccaa ctgatttcta ttggcggagc gattggaacg gggttgtttt
    19681 taggttccgg aaagtctatc cagctagctg gcccttcgat attgctcgcc tatcttatta
    19741 ctggatgcat ctgttttttc attatgcgtg cgttaggcga acttctgtta tcaaacacca
    19801 ataaccattc ttttctggat tttgttgcag aatatcttgg caaaaaagcg gctttcatca
    19861 ctggctggac gtattggttc tgctgggtat cgattgcaat ggccgattta acagccatcg
    19921 gaatgtatgt acgctactgg gcgcctggtg ttcctcagtg gcttcctgaa ctgattgcat
    19981 taggtcttct tctttgcttg aatatggtgg ctgtctcatt gtttggtgag ttagagtttt
    20041 ggtttgcatt gatcaaagtt gtagcgatca ttgcctttat cctcgtagga gcttatatga
    20101 tcctcaccca ctatacgaca gatattggca cagccagtat cacgaatcta tggtctcatg
    20161 gcggattctt tccaactggt gcaaaaggat ttatcttagc tttccaaatg gtaacctttg
    20221 cttttgctgg gatagaatta gttggattag tcgctggaga aacagaaaat cctgaaaaag
    20281 tgttgccgga agctatcaat aatattccta tcaggatcat cttattttac ttaggatctc
    20341 tttttgtcat tatggcaatc tatccatgga acagcttgaa tccagataac agtccatttg
    20401 tagaagtatt ttcggaaatt gggatcacga ttgctgcttc cttgatcaat ttagtcgtat
    20461 taagtgcagc agcttctgcc tgcaacagtg caatatatag tacaggacgt atgcttcgtt
    20521 ctttagcgca agaaggcagt gcccctaaaa aattcaaaaa gttaacaaca catcatgtgc
    20581 cagggaatgc tttgacattt tctactatcg tcattttcat ttctgttatc ttgaattacg
    20641 tgatgcctag tgaagtattc acactagtaa gcagtattgc cactacttgt ttcttgttta
    20701 tctggagtat gcttgtctac acgcacatga aatatcgtaa aagtatcctc ggcaaaaaag
    20761 ctcattcatt taaaatgccg ttgtacccat ttagtaatta tctggtattt ctttacatgg
    20821 cctttgtgtg tgtcgtcttg ttcctaggaa aagatacgag aatcgcttta ttactaacac
    20881 ctgtgtggtt cttgcttcta ttactaatct atcatatgaa atatgaacgt aagaagtaag
    20941 aaagtagttg tgacagaagt ggtcagcttc gagaaataag aaggaattta cgaaaattgc
    21001 ttcttaaatt tttgtgaaat ttcggcttat ttcttggaga taaaagcttc tgtcccagcc
    21061 tcttttctat ttccccggaa acaaccgctg ataataggca tctgcttttt tataggaaaa
    21121 aatccaaata ccagatacaa aaaaacgtcc gaataacccg atcaacggta caaaaaagac
    21181 accggcaatc aagcataagt taatcatcat ggagataaga cttcgacggt cctttgacaa
    21241 aactctcacg acatgggaat ctggtttatt tgctttcatg atttgcctca tcaagatacc
    21301 aaaagaaaaa ctccacatcg tgtatactaa gacataagcc atctcaggcc aaaaagcttc
    21361 agggttacgc ccgacccaac tagtaacaga aggacataaa gtcagccaaa agagcaaaaa
    21421 gatattcgac cagaccactc ggtaattaat tcgttctgcc atctgaaata tacgatgatg
    21481 gttcacccag atcaccgcaa caaatataaa gctgatggta taagctaata atgtatttcg
    21541 gatgacccat aaagaatgaa agctagatgt attagggatc ttgatctcta atacaataac
    21601 tctcaaaaca atggctacca ctccatcagt aaatgcttct aatctcgact ttgtcacgat
    21661 gattcctcct cagatgattc agcgctttta ttttatcact tacgttatta aataaccatc
    21721 gttttatata gggaattttc tgaaaattgt tgcatatttt tcgaaatttt gttactttta
    21781 aaaaaaccgt aaaggagtta tgtatgacaa aaaaattatc atttaaagac tatcttttta
    21841 taggctccat gcttttcggt ctattttttg gtgctggaaa tcttatcttt cccgttcatt
    21901 tgggtcaaga agcaggcgct gctacctttt gggcgaatct aggtttcttg gttaccggta
    21961 tcggtcttcc cttcttaggg gtgattgcca ttggtgtatc gaaaagctca ggtgtatatg
    22021 aattagcaaa acggataaat caaacatatg cgatggtttt taccatcttg ctttacttag
    22081 ttatcggacc gttttttgct ttacctcgcc ttgcaaccac atctttcgag attggtttag
    22141 caccatttat cgatcctagc agacagacgc tatatctagc aattttcagt attgtatttt
    22201 ttgtgctggc ttggtggttt tcaagaaaac cgacaaagat attagattat gttggaaagt
    22261 tcctgaatcc tgcatttttg gttttattag gtgctttgct tctccttgct tttttgaatc
    22321 cactaggttc tgtaaatcaa gcgcctattc agccaaatta tcaagagcat gcttttttca
    22381 cagggtttac tcaaggatat aacacattag atgcgcttgc tgctttagca tttgggatca
    22441 tcattgtcac aacaatccaa aacatgggtg taacaaaacc tgctgaaatc gctaaagaca
    22501 cgatcaaatc tggtgcgatc agcattatat tgatgggaat catttatact ttgctcgctt
    22561 atttaggtgc tatgagctta ggtagctttg ctctaagtga aaatggcggg atcacgctgg
    22621 ctcagattgc tgatcattat ttaggtactt atggcagtat cttattagca tttatcgtta
    22681 tattagcttg cttgaagacc ggtattggtt tgattacagc ttttgcagaa acattcacag
    22741 atttgtttcc gcaaagaaat tacgcattct ttgttgcact tgctagtatc cttccttgtt
    22801 tagcggcaaa cgtaggcttg acgaatatca ttcaattttc gttgcctgtt ttgatgttca
    22861 tctatccatt agcaatgaca ctgattttac tagtgttagt tggtcctttg tttaagcagc
    22921 gtacggctgt ttatcgaatg actacttatt ttacattgat tgcttcgatt tttgatgggt
    22981 tgaacgcttg cccagaaact atcaaacaaa cacctattgt tcaaaatctt ttatatgttg
    23041 cggaaagcta cttgccgttt ttcaaattgg ggatggggtg gatcgtacca gcagttatcg
    23101 gttttgttat cggactgatt tggagtttcg ctaagaaaga agaagtagca gattgataaa
    23161 agaggttgtg aaagagacgt tcagccttga gcaataaaaa gtaattttga aaaatggctt
    23221 ctcacatttt tgcataaatt tgacttaata ccgagaaact gacttttgaa caccgtttat
    23281 tgagaggtcg tgaaaaagcc gcttagtttc aagtattaag aaatattttg tgaaaatggc
    23341 ttctcacatt tttgcataaa tttgacttaa taccgagaaa ctggcttttg aacaccgttt
    23401 attgagcgtc tataaactga cctgagtatt agagaaacaa aacggcaaag tagctccata
    23461 tactttgccg ttttgttttg aactaatacc gaagagaagc tgatcaaaca acatttcttt
    23521 tataatatag attttagaca tatatatgat ataaaaatag ttttcgcaaa a
//
LOCUS       DACGLE010000039        23490 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0534
            SAMEA3304449-rid9560453.denovo.040, whole genome shotgun sequence.
ACCESSION   DACGLE010000039 DACGLE010000000
VERSION     DACGLE010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304449
            Sequence Read Archive: ERR985064
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23490)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23490)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702225.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702225.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 20:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,045
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 27 of 67
            Pseudo Genes (incomplete)         :: 25 of 67
            Pseudo Genes (internal stop)      :: 19 of 67
            Pseudo Genes (multiple problems)  :: 4 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23490
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304449-rid9560453.denovo.040"
                     /strain="VREN0534"
                     /db_xref="taxon:1352"
     gene            108..971
                     /locus_tag="IXT20_001650"
     CDS             108..971
                     /locus_tag="IXT20_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAQ4860097.1"
                     /translation="MYQIVTDSCCDLPYHVLAKEKVDFLSMNLQLNDQELVDDLGKTF
                     NYDRYMNQLKNGAMPTTSQINVGRYLEFFRSYAEKQIPVLYVAFSSGLSGSYSSALQA
                     VELLKEEYEDPEIYVFDTKAASIGEGLLVLEAVRLKKEGKSIEETLEWLESNYLKVHS
                     WVTVDDLKYLERGGRISKASATLGSLLSVKPIITVDKEGRLTNVDKVRGRNKSIQTIV
                     NETVKTIVAPLDQIVYVAYAGDLESAEKAKRLLEEQIKMKDVKLYPLGPTIASHTGYG
                     CIAIFSMGISR"
     gene            complement(1100..2722)
                     /locus_tag="IXT20_001651"
     CDS             complement(1100..2722)
                     /locus_tag="IXT20_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ4860098.1"
                     /translation="MITVNDVSLHFSDRKLFDDVNIKFTPGNCYGLIGANGAGKSTFL
                     KVLSGDLQPSTGSVTMGPDERMAVLKQNHYDYEDYTVLETVIMGHKRLYEVLKEKDAI
                     YMKEDFTDEDGIRAAELEGEFAELNGWEAEPEAAVLLQGLNIPEELHHQQMSELTGGQ
                     KVKVLLAQTLFGKPDVLLLDEPTNGLDIQSINWLEEFLINFENTVIVVSHDRHFLNKV
                     CTHMADLDFGKIKLYVGNYDFWLESSQLAAKLQANANAKKEEQIKELQEFIARFSANA
                     SKSKQATSRKKMLEKITLDDIQPSSRRYPFVGFKPEREIGNDLLQVENVSVTIDGKKI
                     LDDISFNLSKEDKVAFVAENDITTTTLFKVIMGEITPDTGTVRWGVTTSNSYLPKDTT
                     KEFDTDLTILDWLRQYASKEEDDNTFLRSFLGRMLFSGEEVLKPVNVLSGGEKVRCML
                     SKMMLSKANVLVLDDPTNHLDLESITALNDGLMAFTGSILFASHDHQFIQTLANRIIA
                     VSDKGVIDRADTTYDEFLENADIQKQLNELWA"
     gene            2971..3492
                     /locus_tag="IXT20_001652"
     CDS             2971..3492
                     /locus_tag="IXT20_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4860099.1"
                     /translation="MDEKRTIDWGSLILGILFVLVSLLSFQDPVGNLVAIVVVFAIFA
                     FIKGIFELFVRNRLKELTGYKGKMPLIIGIIDILVGVFFLFNIGAGVAALPFVFAVWF
                     IADSVLALFTADLARGVSEGYYWFTIIVNILGILLGIFLLFNPISSALTLSFLVGFYF
                     MLFGITHIVYAFR"
     gene            complement(3678..5408)
                     /gene="spxB"
                     /locus_tag="IXT20_001653"
     CDS             complement(3678..5408)
                     /gene="spxB"
                     /locus_tag="IXT20_001653"
                     /EC_number="1.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate oxidase"
                     /protein_id="HAQ4860100.1"
                     /translation="MSKINAGIAMVKVLESWGIDHIYGIPGGSFNSIMDALYQEKTEI
                     NYIQVRHEEVGALAAAADAKLTGKVGAVFGSAGPGATHLINGLYDAQMDHVPLVAFLG
                     QVASTSMNYNSFQELNENPIFADVSVYNRTVMTPQSLPHVVDEAIKAAYEHKGVAIVT
                     IPVDLGFEEIDEQPFSTAHTHKTSVILPEEKDLLAALPYLEKAKKPVLYVGQGTRNGF
                     PQIKEFSEHFSVPVVASVLAKGIIPDDYENFLGFAGRVATKPGNEALAEADLILFVGS
                     DFPFGRAFFNPTAEFIQIDIDASKFGRRHETSLSILGDANTALQRLVELGDARPADSW
                     YLANQENKEKWVNWLKSFEDREEEPIRPEAVYKEINRIAEDDAIFVTDVGNTTIHSIR
                     LLNMNGKQKHTTSGWFATMGNGVPGGIAAQLSYPEKQVFTLSGDGGFAMVMQDIITQV
                     KYQLPIINVVFSNDSFGFIEAEQEDTEQKKFGVFLEDADFGKVGEALGANSFTVTEYS
                     QLRFAFDAAAKSDRPVVIDVKINNKRPLPVEDLHLDPRKYSEEEIQAFKEKYEVHDLP
                     VLNELYEKRL"
     gene            5573..6391
                     /locus_tag="IXT20_001654"
     CDS             5573..6391
                     /locus_tag="IXT20_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005232914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HAQ4860101.1"
                     /translation="MVEWLLRLEPWQQALAGTAFTYFMTALGAGLVFFFKEIKKEVLN
                     LMLGFASGVMIAASFWSLLDPAITKAEENGDIAWLVVSIGFGLGGVFLYMADKTLPHM
                     HFGPQHEAEGLPTHLKRTILLVFSITLHNIPEGLAVGVAFGAAATADNPTAAILAAVS
                     VALGIGIQNFPEGAAVSIPLRQEGLSRKKAFVYGQASGIVEPIAGVIGALLVTKVELL
                     LPYALAFAAGAMIYVVVEELIPEAQQTLSSKRHFAVFGVMSGFIIMMILDVALG"
     gene            complement(6494..6691)
                     /locus_tag="IXT20_001655"
     CDS             complement(6494..6691)
                     /locus_tag="IXT20_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4860102.1"
                     /translation="MRQKNMFLNIFMVLLVLIAASFVISLFVSVLSGLLWFAVKVLIP
                     IALIIWLVRWIKGNTHRKNYY"
     gene            6860..7438
                     /locus_tag="IXT20_001656"
     CDS             6860..7438
                     /locus_tag="IXT20_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1211 domain-containing protein"
                     /protein_id="HAQ4860103.1"
                     /translation="MPKNRLEAFTDAVIAIIMTILVLELHEPNGDTWEALAALGYRFF
                     IYIVSFIILAIYWNNHHHLFQVVHKINGRVLWANSFFVFALSLFPFATSWISEYINSL
                     APEMTYGFVTLTANIAYYILAKELVKANKGHEKALKAMLNNYKKSYLSIGLNVLGIVL
                     GYLVAPVAVLIANIVILAAWIIPSRTIESQYR"
     gene            complement(8483..8986)
                     /locus_tag="IXT20_001657"
     CDS             complement(8483..8986)
                     /locus_tag="IXT20_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HAQ4860104.1"
                     /translation="MRYVSKFLLGIYLFFLLWLILFKLSFNLPQFLTYSYSNVNLVPF
                     STFSFENTTVLRETTYNLIVFFPFGVLLNVNFKRLSFSKKLGIIFLVSFLAELIQFLF
                     GIGVADITDLITNTTGGLIGLWAYQLLNKHLSTNKLDRLAIILGYILFLFLIVAFLFL
                     TRTVRLQ"
     gene            9345..10370
                     /locus_tag="IXT20_001658"
     CDS             9345..10370
                     /locus_tag="IXT20_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAQ4860105.1"
                     /translation="MFKTYFRFNKIQDNPFRIMNLMLLIFIVLWLGYINHDFLISSFS
                     SLGIFTFFYYQNIPMKYLLKRMFLIGCGLLVAFVLGILSTYVLWLEPFAVALVAFSSR
                     FVLRLFHISKPGGLFFAMLSAMGTSMQLPIAQLPIVSLYFFMGVVFALIAAVITKLLD
                     SRPEQTIEKATLKERFHEEPLVIIDSVFYSAALFLSVYVSHGLNLHNPYWLTLSCASI
                     LLAENLDAMKHRQVQYLIGSMGGLCVSAFLSFVPFTQLQTIFLITFLYGIAQFLVARN
                     YAVANIFLNPMALMLSTLIRGAYLISLIEYRFLGIVIGSFIGLGVAWVMTVGLQHYLT
                     VVRQKLE"
     gene            10717..11595
                     /locus_tag="IXT20_001659"
     CDS             10717..11595
                     /locus_tag="IXT20_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4860106.1"
                     /translation="MRFKDMKRGNLMKKISNEIQILAETLTLPKVVIEQMIVQAKNIL
                     DEVFEQYSHELMDPNKAKATAKKIDKVYEEEPFAALAIYLYAASQSWKKIYQPLKIPR
                     TIYLATMNAFTRFIHEHFQVTGSYRFDRGFWIWHYTSGLIFRIGELEYEMTYFPKGYT
                     DLRLEGKQCLSLHIPSDADLSQNKIRDSYESAIRFFELYFPDYHYQVMYTDTWLLSPN
                     LTKWLKKESKICLFAADYRLLSVDEQDDSGVPWIFGRVDAQIHDYPESTSLQRQAKEQ
                     LLAGEHIGSGLGILTS"
     gene            12071..12187
                     /locus_tag="IXT20_001660"
     CDS             12071..12187
                     /locus_tag="IXT20_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002383181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HAQ4860107.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKDVKRNSGKR"
     gene            13115..13453
                     /locus_tag="IXT20_001661"
     CDS             13115..13453
                     /locus_tag="IXT20_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4860108.1"
                     /translation="MTQPVSSEEHDNLFRATEEFKKYQENIVPETPLASEPITDIDGN
                     QIGWYIQYEVMVVNNDSIVKNTVGLELTPIEKVSSLLTYIYTDESDETEIILTDYSKI
                     ATDKKYILWM"
     gene            13547..13777
                     /locus_tag="IXT20_001662"
     CDS             13547..13777
                     /locus_tag="IXT20_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4860109.1"
                     /translation="MLVEKGKENIYYVNVAKVREDENEWKEFKSRYSINSTPTFTVYR
                     EGSIEKTVFWTKESGMSLAEVEEFLDYVSMQQ"
     gene            14177..14548
                     /locus_tag="IXT20_001663"
     CDS             14177..14548
                     /locus_tag="IXT20_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/Cl family transcriptional regulator"
                     /protein_id="HAQ4860110.1"
                     /translation="MRDYVTDKTQEVINQSQSLNELSDKAKEEAVAIISSGISDYIDM
                     SGEINYGDKDKIVRMIKAFIKTFDVKADLKFNDTYLVGKLINILGTEKDTETFISSLS
                     MLFTDKHFSTQLGGEELVEIF"
     gene            complement(14822..15514)
                     /locus_tag="IXT20_001664"
     CDS             complement(14822..15514)
                     /locus_tag="IXT20_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAQ4860111.1"
                     /translation="MKGIIFDFNGTMFLDSHLHEAAWLHMIHDHTKDGLSDEDILKNI
                     HGRTNTEILKHFISEHLTASEIAVLSEEKESYYRMLCLQNENELQLTKGLEATLDQLT
                     DQQIPLTIATATVKENVAFYFDIFDLARWFDWDKIVYDDGSFPGKPQPDIFLKAADKL
                     SLGPADCLVIEDAYSGLLAAKRAGIGTIIAIDPFGKNKTVFEQAQLNKDGTIKDFTGF
                     WTEHLSATSVNS"
     gene            complement(15517..16302)
                     /locus_tag="IXT20_001665"
     CDS             complement(15517..16302)
                     /locus_tag="IXT20_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAQ4860112.1"
                     /translation="MLKKERLLTIVEMVNKKGILTVNEIINQLDVSDMTVRRDLDELE
                     KSGKLLRVHGGAQSLSYTLDQELSHTEKATLQIQEKKEIAATAASLIHEGETIFLGPG
                     TTIELLATHLLNKKIRIITNNYPVFDILAQADGPEILLIGGDFRKNTGAFVGPITNDN
                     LRRLKFTKAFISSNGVHNEAISTYSTEEGEAQQIALNNSRKKYLLVDNKKFNRDDFYV
                     FYNLHDFDLLITDRQISREVQEHYAQYVDLQIADSTENTTIGA"
     gene            16539..17939
                     /gene="lacG"
                     /locus_tag="IXT20_001666"
     CDS             16539..17939
                     /gene="lacG"
                     /locus_tag="IXT20_001666"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HAQ4860113.1"
                     /translation="MLKLPEDFIFGGATAAYQVEGATKEGGKGAVAWDDFLEEQGRFS
                     PDPASDFYHQYAKDIELCERFGVNGLRLSIAWSRIFPDGAGKPNPEGIAFYHRVFEEC
                     KKRNVTPFVTLHHFDTPKRLFDHGDFLNRETIEAFVSYAIFCFHEFKEVKVWSTFNEI
                     YPVATNQYLLGVFPPGIKYDFTKIVACLHNMMVAHARVVNYFKENELPGEIGVVHSLE
                     TKYAATDAPEDKHAAFLDDALSIRFLLDATYLGYYSTETLTALDEICEANQASYHFPE
                     EDFVELKKASTRNDYLGINHYQCHFVKAYDGENAIHHNGTGEKGTSVYKVKGIGERIY
                     KEGIPRTDWDWLIYPEGLYDLLLRIKSDYPHYNKIYITENGMGYKDQFEDGIIMDQPR
                     IDYLRVYLESLSKAITAGVNVKGYFLWSLMDLFSWTNGYNKRYGLFYVDFETQKRYPK
                     ESAYWYKLVSETKTII"
     gene            17964..18281
                     /locus_tag="IXT20_001667"
     CDS             17964..18281
                     /locus_tag="IXT20_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HAQ4860114.1"
                     /translation="MNKEELQMLGFEIVAYSGDARSTLLKLLKEVRQGNFEHVDSALK
                     EADENLTLAHNSQTKILAEEASGKEMEMGFIFIHGQDHLMTTLLLRDLIQDFIVLYRQ
                     NQG"
     gene            18294..19967
                     /locus_tag="IXT20_001668"
     CDS             18294..19967
                     /locus_tag="IXT20_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ4860115.1"
                     /translation="MDGIIKQIEKGKPFFEKISRNSYLGAVRDGFLTAMPAILFSSIF
                     ILIAAVPEIFGYQWPESVSTWLWKVYNYSMGVVGLLVSATTARCLADSMNRRLPKNKV
                     INTTSVMLASVVSFLLLSVSPIEGGFSTEYMGTKGLLASFVAAFLTVNLYKFCVLKDI
                     TIKMPKEVPGTISQTFRDIFPFSFSVFAAVLIDLGIRQIFQISFAEAIITVLQPLFTA
                     ADGYLGIAIIWGAMALFWFVGVHGPSIVEPAIAAIIYANVETNLQLFNSGEQASKVLT
                     VGLGNFVGTMGGTGATLVVPFIFLLFAKSKQLKAVGKASFIPVSFAVNEPLLFAAPMI
                     LNPYFFIPFLLTPVVNVCLFKFFVDVLGMNSFMYVLPWATPAPIGLVLGTGMGILAFV
                     LAAVLIVVDFLIYLPFCKAYDAVLVEKEAQKAEVIATEEGASADVREEVPVAASQVIS
                     DANKEINVLVLCAGAGTSAMLANALKKGASESKAPISAMAGAYGSHYEMLQDFDMVVL
                     APQVNSYYDDIKEDTDRLGIKLVATKGAEYIGLTRDPEKAVQFILNQFQ"
     gene            19981..21612
                     /locus_tag="IXT20_001669"
     CDS             19981..21612
                     /locus_tag="IXT20_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016608433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinogalactan endo-1,4-beta-galactosidase"
                     /protein_id="HAQ4860116.1"
                     /translation="MKMGIWLRKAVLFAAVLGALGAVSKTVEASASEFIRGADISILS
                     DMEKSGAKYYEDDISKDALEILKNNGVNYVRLRLWQDPYNSNGESYGAGTNDLETTID
                     LAKRAKNLGLKVLLDFHYSDFWVDPGKQNLPKAWQGLTFEEMNTALYDYTKNVLSEMK
                     QLDVYPDMVQIGNELNSGMLWPYGKSWGEGGGEFDRLAAFLKSGIQAVRDTQPKTTPV
                     MLHLADGGDTGAFTWWFDEITSRGVSFDLIGVSYYPYWHGSMNELQTNMNAISQRYGK
                     EVIVVETAYGHTTANADTMPNAFGEAEAAAGGYEPTPTGQAEYLLDLADRIQAVPNNR
                     GAGFFYWEPLWYNGNVSWATQAGMNYLSVQSTMGNEWDNQAMFDFAGNALPSLRAFKQ
                     AGAQINLVKNASFESDGPTTSPSNWQTWFQQGTAKGTIKTEAGTFFGDYKLTFWQDQA
                     YEASAYQNVSGISKGTYTLSAYVMSGGEFDTNQLYIKNYGGAELNTAVVKTSEWTKIT
                     IKDIPITTGVCEIGVYSKASGNAWTNVDFVSLVKQ"
     gene            22484..>23490
                     /locus_tag="IXT20_001670"
     CDS             22484..>23490
                     /locus_tag="IXT20_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISLgar5 family transposase"
                     /protein_id="HAQ4860117.1"
                     /translation="MNDFTTEILKTLANKGDLNELFRVHLEKAVNTLLKTELTAFLDY
                     EKYDRIGFNTGNSRNGSYDRTVKTEYGELHLQIPRDRNGEFKQQTVPAYRRTNDTLEE
                     TVIHLFRKGITMSEIADLIEKMYGHYYTPQTMSNITKSFTEEVTAFNGRELHDRYAAI
                     YMDATYIPLKRKTVAKEAIHIAVGIRPDGSKEVLSYAIAPTESITIWEEILLDLQERG
                     LKNVLLFITDGLKGMVGAISRFYPKARFQHCCVHVSRNISHKVRVDDRKEVCDDFKMV
                     YQASSKEVALEARGTFAEKWKTSYPKVVESILSNDHLLTFYDFPLAIRKSIYSTNLIE
                     S"
BASE COUNT         7329 a         3961 c         4758 g         7442 t
ORIGIN      
        1 attttgatta gaacttgcga aaataaaccg gttaaagtgt gtctagcgca agagatttgc
       61 tataatagac gataagggta aaatgtaacg agaaggggtt gaaaaatgtg tatcaaattg
      121 taacagattc ttgttgtgat ctgccatacc atgtattagc aaaagaaaaa gttgattttt
      181 tatcaatgaa tctccaattg aatgatcaag agttagtaga tgacctcgga aagacattca
      241 attatgaccg ctacatgaat cagctgaaaa atggtgcgat gcctacgaca tcacaaatca
      301 atgttggccg atatttagaa tttttccgtt cttatgcgga aaaacagatt cctgttttat
      361 atgtggcttt ttccagtgga ttaagcggct cttacagtag tgcgcttcaa gcggttgaat
      421 tgctgaagga agaatatgaa gaccctgaga tctatgtttt tgatacaaaa gctgcaagta
      481 ttggggaagg gcttttagta ttggaagctg taagattgaa aaaagaaggt aaaagcatcg
      541 aagaaaccct agagtggctg gaaagtaatt atttgaaagt ccattcatgg gtaacagtag
      601 acgatctgaa atatctagaa cgaggcggta ggatttccaa agcaagtgct actcttggca
      661 gtctattgag tgtaaagcct attattacgg tggataaaga aggaaggctg acgaatgttg
      721 acaaagttcg aggaagaaac aagtcgatcc agacaatcgt caatgagacg gtaaaaacaa
      781 tcgttgcacc acttgatcaa atcgtttatg tggcttacgc tggggatcta gagagcgcgg
      841 agaaggcaaa acggcttttg gaagagcaga taaagatgaa agatgtgaag ctttatccct
      901 taggaccaac gatcgccagt cacacaggct atggctgtat cgcgattttt tcgatgggaa
      961 tcagtcgata aaagcagtaa ctcattctgt aaaaaagccc agaaattgat tgtaacaaaa
     1021 acagcgactc ttaaaagata gatgagcatc ctatctctta gaggtcgctg tttttttatt
     1081 ttttaagcta aataggccat tatgcccata actcgttcaa ttgtttttga atatctgcat
     1141 tttccaaaaa ttcatcatat gtcgtatcag ctcgatcgat cacaccttta tccgaaacgg
     1201 caatgatgcg gtttgccaat gtttggataa attgatggtc atgtgacgca aataagattg
     1261 agccagtaaa tgccatcaag ccgtcattca atgctgtgat tgattctaag tctaaatggt
     1321 tcgttggatc atctaataca agtacgttag ctttcgaaag catcattttt gaaagcatgc
     1381 aacggacttt ctctccacca gataaaacat tcactggttt caatacttct tcaccagaaa
     1441 acaacattct tcctaagaag cttcttaaga aggtgttatc atcttcttct ttacttgcat
     1501 attgacgcaa ccagtctaaa atcgttagat cagtatcgaa ttctttcgtt gtatcctttg
     1561 gtaaataaga attacttgtt gtcacacccc aacgtactgt tccagtatct ggcgtaattt
     1621 cacccatgat aactttgaat agcgttgttg ttgttatatc attttctgcg acgaaagcga
     1681 ccttgtcttc tttgctcaaa ttaaaagata tatcatctaa aattttcttt ccatcaatcg
     1741 ttacacttac attttctacc tgcaataagt cgtttccaat ctcacgttca ggtttgaatc
     1801 caacgaatgg ataacgtcta gaagaaggtt ggatatcgtc taaagtaatt ttttcaagca
     1861 tttttttacg tgaagttgct tgttttgatt ttgatgcatt cgcactaaag cgggcaataa
     1921 attcttggag ttctttgatt tgttcttctt ttttggcatt tgcgtttgct tgaagtttag
     1981 ccgctagttg actagattcc aaccaaaaat cataatttcc tacataaagt ttgattttac
     2041 caaaatctag atccgccata tgtgtacata ctttgttcaa aaagtgacgg tcatgggata
     2101 caacgatcac agtattttca aaattgatca aaaattcttc cagccaattg atcgattgga
     2161 tatccagacc gtttgttggt tcgtctagca aaagaacatc aggtttacca aatagtgttt
     2221 gtgctaataa cacttttacc ttttgtccac cagttaattc actcatttgc tgatggtgca
     2281 attcttcagg gatgttcaga ccttgcagta aaacagctgc ttctggttca gcttcccatc
     2341 cattaagctc agcaaattct ccttctaatt cagctgctcg aataccatct tcgtcagtga
     2401 aatcctcttt catgtaaatc gcatcttttt ctttcagcac ttcatacaga cgcttgtgtc
     2461 ccatgatgac tgtttcaagt acagtatagt cttcatagtc gtaatggttc tgtttcaata
     2521 cggccatccg ttcatctggt cccatagtaa cggaacctgt ggaaggctgc agatcccctg
     2581 ataacacttt aaggaacgtt gatttacctg caccatttgc accaatcaag ccatagcagt
     2641 ttccgggggt aaatttaata ttcacatcat caaatagttt gcggtctgaa aagtgcaaac
     2701 tcacatcatt tacagtaatc aatttttttc ctcactttta tcattattag ttttgacaag
     2761 tcttatagaa ttatataggg gaaaggtaaa agattcaagg caatcgaaca ttctttgtgt
     2821 ttcacgtgaa acagaatgaa aaaaataaaa ataaaagcgg agcgaaaata gctactatag
     2881 gatttattag tttgcaacag cataattttt gacgttattt ataagaggga gtactataaa
     2941 actgtaaaag aaatgaaaga aggattgaaa atggatgaaa aaagaaccat cgactggggc
     3001 tcattgattt tgggaatttt atttgtactt gtctcattgt tatcatttca agaccctgtc
     3061 ggaaatttag ttgcaattgt agtagtcttt gcgatttttg cctttattaa agggatattt
     3121 gaactgtttg tacggaacag attaaaagag ctaacaggat ataaaggaaa gatgccctta
     3181 ataatcggga tcatcgatat tctagttggc gtctttttct tattcaatat tggtgcagga
     3241 gtcgcagcat tgccttttgt ttttgcagta tggtttattg ctgattcagt tttagcgtta
     3301 tttactgctg acttggcaag aggagtaagt gaaggatatt attggtttac gatcatcgtc
     3361 aatattttag gaattttatt aggaattttc ttattgttta atccaatttc ttctgcgttg
     3421 acattaagct ttttagttgg attctatttt atgttatttg gtatcacaca tatcgtctat
     3481 gcatttagat aagtaaaatc aaacaaataa aaaagccggc ttccgattac agttttactg
     3541 ttgtaattgg aagccggctt ttttatcatg gacaaatatg cacgaggtga tttttaggtg
     3601 tcgagacaaa tttatgcaaa aatgtgagaa gctatataca caaattttat cttaatactc
     3661 gaagcatagc gcagttgtca caacctcttt tcatataatt cattcaatac tggcaaatca
     3721 tggacttcat atttttcttt gaatgcttgg atttcttctt cagaatattt tcttggatct
     3781 aaatgcagat cttctactgg caatgggcgc ttgttattga tcttcacatc gattactaca
     3841 ggacgatctg attttgcagc tgcatcaaag gcaaatcgaa gttgcgagta ttctgtgact
     3901 gtaaagctat ttgccccgag cgcttctcca actttaccga aatctgcatc ttctaaaaat
     3961 acaccaaatt tcttttgttc cgtatcttct tgttctgctt cgatgaaacc aaaagaatca
     4021 ttcgaaaaga caacattaat aattggaagc tgatatttga cttgtgtaat gatatcttgc
     4081 atgaccatag caaacccgcc atcaccactt aaggtaaata cttgtttttc tgggtagctt
     4141 agttgtgcgg caattcctcc tggcacacca ttacccatag ttgcgaacca tccagatgtc
     4201 gtatgttttt gtttaccgtt catattcaat aaacgaatag aatggatagt cgtgttaccg
     4261 acatctgtga cgaaaatcgc atcgtcttca gcaatacgat tgatttcttt atagacagcc
     4321 tctggacgaa tcggttcttc ctctcgatct tcaaaagatt tcaaccaatt tacccatttt
     4381 tctttatttt cttgatttgc taggtaccag ctatcagcag ggcgagcatc ccctaactcg
     4441 acaagtcttt gcaaagctgt gtttgcatca ccgaggatcg acaaagatgt ttcatggcgt
     4501 cgtccaaatt ttgacgcatc gatatcgatt tggataaatt cagcagttgg attaaaaaat
     4561 gcacgtccaa atgggaagtc gctaccgaca aataagatca gatcggcttc tgccaatgct
     4621 tcatttccgg gtttagtagc gacacgtcca gcaaaaccaa gaaagttttc ataatcatca
     4681 ggaataattc cttttgctag aacggaagcg acaacaggaa ctgaaaaatg ttctgagaat
     4741 tctttgattt gcggaaatcc gtttcgcgtt ccttgaccaa cataaagaac tggttttttt
     4801 gctttttcaa gataaggcaa agcagctagc aaatcttttt cttctggtaa tatcacactt
     4861 gttttgtgag tgtgagcggt ggagaaaggt tgttcgtcaa tttcttcaaa accaagatca
     4921 acagggatag tcacgatagc cacgccttta tgctcatagg cggctttaat ggcttcatct
     4981 actacatgcg gaagactttg tggtgtcatg actgtacgat tgtatacgct aacatctgcg
     5041 aaaataggat tttcatttag ttcctggaaa gagttatagt tcatggatgt agaagcaact
     5101 tgtcctaaga acgctactag aggcacatgg tccatctgtg catcatacaa accattgatc
     5161 aaatgtgtcg caccaggtcc tgcagagccg aagacagcgc cgactttccc cgttaatttt
     5221 gcatctgcag cggctgcgag tgcgccgact tcttcatgac gaacttgaat gtagttgatt
     5281 tcggtttttt cttgatatag cgcatccata attgaattga aagatcctcc gggaatgccg
     5341 taaatatggt cgatacccca gctttcaaga acttttacca ttgcgattcc tgcattgatt
     5401 tttgacatgg tgtttcactc ctttagcaat gatgatacat aaattttttg tatctagagt
     5461 atagaacttt ttatcgaaaa tcccaactaa ttcgtctttt cacaaaaatg tagtcatgtt
     5521 tttgggaata tgctagagta gtacatagat agattcaaga gaggggaatg gcatggttga
     5581 atggctattg agacttgaac cttggcagca agcacttgct gggacagctt ttacttattt
     5641 tatgacagct cttggtgccg ggttggtttt tttctttaaa gaaatcaaaa aagaagtgct
     5701 aaacttgatg ttaggatttg cttctggtgt catgattgct gcaagttttt ggtctttgtt
     5761 agatccggct attacaaaag cagaagaaaa tggagatatt gcttggctag ttgtaagtat
     5821 tgggtttggt ttaggcggtg tgtttttata tatggcagat aaaacactgc cccatatgca
     5881 ttttggacca caacatgaag cagaaggatt gcctacccat ttaaaacgga cgatcttgct
     5941 tgttttttct attacattgc ataacattcc tgaaggttta gccgtggggg tagcattcgg
     6001 tgcagcagca actgcggata atccgacagc cgctatatta gccgctgttt cggtggcgct
     6061 aggtattggt atccaaaact tcccagaagg agcagctgta tcaatcccgt tgcgacaaga
     6121 aggtctgagt cgtaaaaagg cctttgttta cggacaagct tctggaatcg ttgaaccaat
     6181 tgctggagtg attggagcgc tactagtaac gaaagtggag ctgctgcttc cttatgcact
     6241 tgcttttgcc gcaggagcaa tgatttatgt cgtggtggaa gaattgatcc ctgaagcaca
     6301 gcagacattg tccagcaaac gtcattttgc agtttttggc gtcatgagtg gatttatcat
     6361 tatgatgatt ttggatgtgg cattaggata aataaaaaaa atatatatga tatatcaaat
     6421 agggccgcga acttatatcg cgaccctatt tttttagtaa gttatatcat aatgaagtga
     6481 atggtatcat acattaataa tagttttttc tatgtgtatt tcctttgatc cagcgaacaa
     6541 gccagatgat aagtgcgatt gggattagaa ctttgaccgc aaaccataaa agcccgctta
     6601 ggacactgac aaaaagactg atgacgaagc tagctgcaat tagcactaaa agaaccatga
     6661 agatatttaa gaacatattt ttttgacgca tacaaaaccc tcctcttgtt cattctttac
     6721 tatagaaaaa gtataacagg gattgtttaa aaacacctcc aactgaagac tgatttttac
     6781 ttcatctact taaatgatca aacaattgct ttttctctgg tttatcatct aaaatagagc
     6841 ggaaaaggag gagaatatca tgcctaaaaa tcgtcttgaa gcatttacag atgctgtaat
     6901 cgcgattatc atgactattt tagttttaga acttcatgaa cctaacggag acacgtggga
     6961 ggcgctcgct gcattaggtt accgattttt tatctatatt gtcagtttta tcattttagc
     7021 gatttattgg aacaatcatc atcatttatt tcaagttgta cataagataa atggacgggt
     7081 cttatgggcg aacagtttct ttgtttttgc gctttcgctt tttccgtttg caacttcatg
     7141 gatcagtgag tacatcaata gcctggctcc agaaatgact tatggttttg ttaccttgac
     7201 cgctaatatt gcgtattata tcttggcaaa agaacttgtc aaagcaaata aaggccacga
     7261 gaaagcattg aaggcgatgt tgaataatta caagaaatcg tatctatcga ttggtttgaa
     7321 tgtgttagga attgtcttag ggtatttagt agcgccagta gccgtgctga tcgcaaacat
     7381 cgtgattcta gctgcttgga tcatcccaag tcgtaccatc gaatcacaat atcggtaaaa
     7441 tgatccgtgt ttaaaattta agagaaaaat atagaaaaaa taaataaacg atcaaatatc
     7501 cgagtgatac tagtattcaa agagggatgc agatggatca aaaaagaatt catgtaggac
     7561 tgattactag ttttgtcacg atgcttattt atttggatca aggtcatttg acttggacag
     7621 tagttggtgt cgtaggattt ttgacattat tcacagcaat aaaaaaataa acatctttat
     7681 gtaatctgat gttctatcta tttaagaaaa gcatgtttac agcagtatga ttctgttgta
     7741 aacgtgtttt tatttgtgga aaatttaatt tttagaaaag tgtatcgaaa aatagatagt
     7801 tagtttttcc acagtggata acttcgtaag agataaaaat aaaaaattag tcaaagaagg
     7861 gcgtatagat ttcttttgat aggagatata caacagaaat gaataaatat taacgtgttt
     7921 tcaaacagta taaaacggtg tggtttttgg aataaaaaaa taacatatct cattttacgc
     7981 ttttgttatt ttatacttaa aaaataacga cacagaaaat accgtgtctc agatttctct
     8041 actacttcta aaaactactg tttttttcct ttgaaatgcc ttcaaaaagt aggatcaggc
     8101 agaatatact ggcagaatca aggagaaggg agcctaaata aggagaaaaa ccatagaaaa
     8161 tataaagtaa ttacttataa aacaagcttt ttatagaaaa ataatatacc tgagtagggt
     8221 ataaattaag acggaattcc tttctagatt gagatattga ctaggaagaa tgttaagatt
     8281 ttaagccttt aagctaaaaa aaaacccttt ttgttaaata aggggaatat agcctgtcag
     8341 tttcaaactg caagtcgtcc tgctgcaata atatagtaga ttagagatga aaatagattt
     8401 ttttaaacca atgactgaat aaaaagaccg tcatagatac acattggaat gtgtctatta
     8461 cggtcttttt ttacgaacga ttttattgaa gacggacagt tcgggtcaaa aataaaaaag
     8521 ctacgatcag aaataagaat aaaatgtatc ccaaaataat ggctagacgg tctaacttat
     8581 ttgtcgatag atgtttgttt agaagttgat aggcccataa acctatcagt cctcctgttg
     8641 tattagttat caaatcagtg atgtccgcta cgccaatacc gaataggaat tgaatcaatt
     8701 ctgccaagaa gcttacaagg aaaataatcc ctaatttttt agaaaaagaa agtctcttga
     8761 aattgacatt caaaagcacc ccaaaaggaa aaaagacaat caagttataa gtggtctcac
     8821 gtaataccgt cgtattttca aaagaaaaag tagaaaaagg gacgagattg acattgctgt
     8881 aagaataagt gagaaattgt ggaagattaa atgatagctt gaataaaatc aaccataata
     8941 gaaagaataa ataaatccct aataaaaatt ttgatacata ccgcatagac atctcctcca
     9001 tttaaaaagt ataccatcga aaaccgctga aaaatcataa aaaagtatga agtttaagga
     9061 ccaacgtagt tatcgtacga aaattagaag cttttttttg ttgggaatcc ggtttcttta
     9121 acaaagctaa gatagcgagt tgcttggaaa agttgctatc ttttttctgt caaaacgact
     9181 cggaatgttc gttttttgtt ctaatttctc caagttgttt actttttgat actaagttta
     9241 tgaaaaatca agaaatagtt gttatactag gtaatgctga caaacgggat tggcatttca
     9301 tcttggttca gctttttttc caattactag gaaggaggga tagattgttc aagacttatt
     9361 ttcgtttcaa taaaatacaa gataatcctt ttcgtatcat gaatctgatg ctacttatct
     9421 ttattgtatt atggttaggt tacataaatc atgatttttt gatttcaagt ttttcatcat
     9481 tagggatctt tacttttttc tattatcaaa acataccgat gaaatatttg ctgaaacgta
     9541 tgtttctgat cggttgcgga ctgttggttg ctttcgtttt ggggatctta tcgacttatg
     9601 ttttatggct tgaaccattt gccgtagctt tggttgcttt ttccagcaga ttcgtgttgc
     9661 gattattcca tatttctaaa ccaggtggct tgttttttgc gatgctttct gccatgggaa
     9721 cgagcatgca gctgccaatc gctcaattac cgatcgtctc actttatttt tttatgggtg
     9781 tcgtttttgc cctgattgcg gcagtgatta caaaattact ggattcacgg cctgagcaga
     9841 caattgaaaa agcaacttta aaagaaagat ttcacgaaga gcctttagtg atcattgatt
     9901 ctgttttcta cagcgctgcc ttatttcttt ccgtttatgt cagtcacggc ttgaatctgc
     9961 ataatcctta ttggttgacc ttgtcatgtg catcgatttt gctagcagaa aacttggatg
    10021 cgatgaagca tcgacaagtc caatatctga ttggatcgat gggtgggtta tgtgtgtcgg
    10081 cattcttgtc atttgtgccg tttacgcagt tgcagaccat ttttctgatc acttttctat
    10141 atggaatcgc acaatttcta gttgctagaa attatgcagt tgctaatatt tttcttaatc
    10201 cgatggcgtt gatgctatct acacttattc gtggagcata cttgatttct ttgatcgaat
    10261 atcgtttttt agggatcgtc ataggaagtt tcatcggttt aggtgttgcg tgggtaatga
    10321 cggttggttt gcaacattat ttaacggttg ttcgtcaaaa attagaatag aaggacaaat
    10381 atatgtgcca aaagaggtta tgacaagatt tttgtcacaa cctctaattc aaggtgttca
    10441 aaagccagat tctcagcatt aagtcaaatt cacacaaaaa tgtggaaagg aactttcaaa
    10501 aaaattttat atttattcta tcaaaaaatt ttatatttat tctatcaaaa aattttgttt
    10561 atgatggtaa ctgaataaac tttcctgaga tggtcaatgc tttccttgaa agtcttacag
    10621 atattctttc ttcatagaaa gtttaagttt tatagttagt gtacaataaa agtatgttta
    10681 taccatatgg tgtccggata atcagtcatt taaacaatga ggttcaaaga tatgaaaagg
    10741 ggaaatttga tgaagaaaat aagcaatgaa atccaaattc tggcagaaac tttaactctt
    10801 cccaaagtcg ttatagagca aatgattgta caagcaaaaa acatccttga tgaggtgttt
    10861 gagcaatata gtcatgaact aatggatcct aataaagcaa aggcgactgc aaagaaaata
    10921 gacaaagtct atgaagagga accttttgca gcattagcta tttatctata tgcagcttct
    10981 caaagttgga aaaaaattta tcaaccattg aagattccaa gaactatcta tttggcaaca
    11041 atgaatgctt ttactagatt tattcatgaa catttccaag taacaggaag ctatcgcttt
    11101 gaccgtggtt tttggatttg gcattacact agtggcttga tctttcggat tggtgaattg
    11161 gagtacgaaa tgacatattt tccgaaaggg tacacggatc taagattaga aggaaagcag
    11221 tgcctttctc ttcatattcc ttccgatgca gatttatctc agaataagat aagagatagt
    11281 tacgaaagtg ccattcgttt ttttgaactt tattttcctg attaccatta ccaagtgatg
    11341 tacacagata cttggttact atcacccaat cttactaaat ggttgaaaaa agagtcaaaa
    11401 atctgtcttt ttgcagccga ttatcgatta ttatcagttg atgaacagga cgatagtgga
    11461 gtcccttgga tctttggacg agtagatgcc caaatacatg attatccaga aagtacgagt
    11521 ttacagcgac aagcaaaaga gcaattgctt gcaggcgagc acattggctc tggcttaggt
    11581 attcttactt cataagttta gtacttcgaa atagtttaaa agagcgtgag acaaaagtaa
    11641 agaacacttt tgtctcacgc tctaaacacg tataaacggc ggaagcagaa gcaactcctt
    11701 cggaaataag ctgaaattca caaaaatttg aaaagcaatt ttcgtgaatt tcttcttatt
    11761 tcttggagtt aaacgcttct gtcccagcct cttttttagt gttctatcga acacacagta
    11821 cgttcaatag gtaaactagc tataacaata gatattaaag ttaacggaca agagggcctt
    11881 ttttaggtag ctaaaacgta ataatgaaaa tttatttcca gagataggag tcaatcagct
    11941 agtctgatcg actgttttaa caagttgact ttcattttaa cttttgatac catttaaaac
    12001 acaaatcgta attgtttcga tttgtgtttt ttagcatgac tacaatcaaa gaagaaagtg
    12061 ggtcgtccat atggaaaaaa cagatatctc aagtgcatac cgccgcttga aaagcccaaa
    12121 tatcaaaaca cgaaaacgtg cgctgaagat catcaaagat gtgaaacgga atagtgggaa
    12181 aaggtaagaa gaaggaaact taattaaaaa aatagccgaa aaacgttttt cggctatttt
    12241 caataatcgg tttttgtgta aggtttccga aagaactgct gttgattttc catttttaac
    12301 atatgataaa ttggtagaat ctttcttttc aaattgatac cattccctaa ttccagttat
    12361 atagttaact acagaaccac aaagcacgta cgtttataaa agtattatct gtagttattt
    12421 ttcaattccc tttcaacctc ttattttgac cagtagtatt gatcacttta ccttttaaca
    12481 cctttgttcg tttttgaaac atttttagtc aagaatggaa acgtaactac ataatcaaag
    12541 gttttgcgaa atatttcatg aaatatttca tgtagaagcg agcaatacgg ttgcaaataa
    12601 ccattatcgt ttaataagca gttacgaaag ctcccaatat ctctaacgtt cagctcactc
    12661 catttctttt cttcaaaaaa atcagtgatt cgtatctctt tctatttgtc gaaaaagtta
    12721 actttaaaaa gtaacgccga aaaaatatgc aaaaaaataa aaaagcagtt actattgcta
    12781 gaataacatc aaggaataat aagttttggt tttcagataa tagtcgataa gttgtgtatt
    12841 ggaattttag atacagtaat aaagtatttt tcatgaaatt ttgtgagaat cactattaca
    12901 agtatcttta ataaaattaa tggttaagga ggaacttaaa gtagaatgta aatgaatagt
    12961 tgtagtcagt cgattgtgat actagactca aagttgcagg aataagtttt tcaatttgtg
    13021 attggaagat aaaaaatgaa aaagaagggc ttttttttat actttgttga ttgtagtgtt
    13081 tattttccct ctgtttagcc aagtaacatt ttctatgact caaccagttt caagtgagga
    13141 acatgataac ctttttcgtg ctacagaaga gtttaagaaa tatcaggaaa atattgttcc
    13201 ggagactcca ttagctagtg agccaattac tgatattgat ggaaaccaaa taggatggta
    13261 tatacagtac gaagttatgg tggtaaataa tgactcaata gtaaaaaata cagtaggctt
    13321 agagctaaca ccgattgaaa aggtgagttc attgttaacg tatatttata ctgatgaaag
    13381 tgacgagact gagatcattt taacggatta ctcaaaaatt gctactgata aaaagtatat
    13441 attgtggatg tagacactag tgaaatactt tatgatatag aaattgatga aaatttacta
    13501 gatgtagcac aagaaatcga tcatcaaaag caggaagcta ttcgatatgc tggtagaaaa
    13561 gggaaaagaa aatatttatt acgtgaatgt agcaaaagtt agagaagatg aaaatgagtg
    13621 gaaagaattc aagagtcgct actcaattaa ctcgactccg acatttacag tttacagaga
    13681 aggatcgata gaaaaaactg ttttttggac taaagaaagt ggaatgtctt tagctgaagt
    13741 agaagagttt ttggattatg ttagtatgca acagtaaggg agttcttatt atgaaaaatt
    13801 ttgacacata ttatgttatt gttttttcga tatctttgct gttaatatta ctaactttaa
    13861 tgcagtataa agagtatcca gagtatttta ttttaacttt tgtatattta gtaagtagct
    13921 acacacttct atataaattc agaagttctt ctagagtttc taaagtttgt ttcttaatta
    13981 gcttattgat tctataatat ttttgattta tataggaaat taaaaatatt atagaatcaa
    14041 cactcagaga acgggttggt ttttaataga tttaataagt actgataatg aaatttctcc
    14101 tggttgggaa aacgaagttg gttaccctgt agaagaattt tttcaagatt actttgcgga
    14161 gtatgccgat aggataatga gagattacgt aacagataaa actcaagaag tcatcaatca
    14221 atcccagtca ttaaatgaat tatctgataa ggcgaaagaa gaagcagttg ccattatttc
    14281 ttcagggatt agtgactata ttgatatgag tggtgaaatc aattatggcg ataaagacaa
    14341 aattgtacgc atgataaaag cttttatcaa aacttttgat gtgaaggcag atcttaagtt
    14401 taatgatact tacctagtag gaaaattaat taatatcctt gggacagaga aagatacgga
    14461 aacgtttatt tcctctttgt ctatgttgtt tacagataag catttttcaa ctcagcttgg
    14521 tggagaggaa ctagtggaaa tcttttaacc attacgccca gcattaataa aagtatatca
    14581 aaaaagtact cctgatgatt tttaagattg gtttgagaaa taatgtgaaa acactatttt
    14641 taatgttaat tatagataga gatgaacgcg tcatatggtt aaaagaatga aaaagtcaaa
    14701 gatacactgc tatatttcac taaggatata gcagtgtatc tttcatgtcg tttcactggc
    14761 agttttttat aaaaggaggt gtgcatttca gttgcttttc tttggtagtt agttgaatcg
    14821 tttaagaatt aacggatgtg gcagacaggt gctctgtcca aaaaccagta aaatctttga
    14881 tggttccatc tttatttagc tgtgcctgtt caaatactgt tttgttttta ccgaatggat
    14941 caattgcaat aattgttccg atccccgcac gtttggcagc aagtaatcct gagtaagcat
    15001 cttcgataac aagacaatca gccggaccta agctcagttt gtctgccgct ttaagaaaaa
    15061 tatccggttg cggtttccca gggaaagagc cgtcgtcata gacaatcttg tcccagtcaa
    15121 accagcgggc taaatcgaaa atatcaaaat aaaaagcaac attctctttg acagtagccg
    15181 tggcaatcgt taatggaatc tgttggtcag tcagctggtc aagagtcgct tcaagccctt
    15241 ttgtcaactg aagctcgttt tcattttgca agcaaagcat acgataatag ctttcttttt
    15301 cttctgacaa tacggcgatc tcagaggcgg tcaaatgctc tgaaatgaaa tgtttcaaaa
    15361 tctccgtatt cgtccgacca tgaatatttt ttaaaatatc ctcgtctgat aaaccatctt
    15421 ttgtatgatc atgtatcata tgcagccaag cagcttcatg cagatgagag tccaaaaaca
    15481 ttgttccgtt gaaatcaaat ataatccctt tcatagttac gctcctatag tggtattttc
    15541 ggttgaatca gcaatttgca gatcgacata ttgtgcatag tgctcttgaa cctcccgcga
    15601 gatctggcga tccgtaatga gcagatcgaa atcatgcaaa ttataaaaca cataaaaatc
    15661 atctcgattg aattttttat tatcaacgag tagatatttc ttacgggaat tatttagagc
    15721 gatctgctgt gcttctcctt cttcggtact gtaagtagag atcgcttcgt tgtgaacgcc
    15781 atttgaactg ataaatgctt ttgtaaactt tagtcttctt aaattgtcgt tggtgatcgg
    15841 tccgacaaat gcgccggtat ttttccggaa atcaccgcca atcagtagaa tttctggtcc
    15901 atctgcttga gcaagaatat caaaaactgg ataattattt gtaatgatcc gaattttctt
    15961 gttcagtaaa tgcgtcgcca atagttcgat tgttgtacct ggtcctaaga aaatcgtttc
    16021 tccctcgtga atcaaggagg ctgctgtggc agcaatttct tttttttctt gaatctgcag
    16081 agtggctttt tcagtgtgag aaagttcttg atctaaagtg taggaaagac tttgagcccc
    16141 tccatggaca cgcagcagct tgcccgattt ttctaattca tctaaatctc tgcgaacggt
    16201 catatcagaa acatctaatt gattgatgat ttcattaacg gtaaggattc cttttttatt
    16261 gaccatttct acgatagtaa gcaatctttc ttttttcaac attgtcttca cctctaattt
    16321 aaggataatc tatcatataa acggacaaca gtcaatctaa ttcgaacaaa agtaaacatt
    16381 tagggtggtt tttatgtttg ttacaataga ttttattgct gttttctggt ttttatgatt
    16441 atttgtgttt gtttttgttg acaaatgttt tggatgcgtt tacaatgcaa tcagttagaa
    16501 agagcgaaaa ctgattatag atttgaggag agatgtacat gcttaaatta ccagaagatt
    16561 ttatttttgg cggagccaca gcagcctatc aggttgaagg agccactaag gaaggtggca
    16621 aaggcgctgt tgcttgggat gactttttag aggaacaagg gcgattcagt cccgatccgg
    16681 caagtgattt ttatcatcaa tatgcaaagg acattgaatt gtgtgaacgt tttggtgtaa
    16741 atggtctaag attatccatt gcttggagcc gcatttttcc tgatggagct gggaaaccaa
    16801 atccagaagg aatcgctttt tatcatcgtg tgttcgaaga atgtaagaaa agaaacgtaa
    16861 cgccgtttgt gacgttgcat cattttgata cccctaaacg tttgtttgac catggagatt
    16921 ttttgaatcg agaaacaata gaagcatttg tttcctatgc cattttttgt tttcatgaat
    16981 tcaaagaagt caaggtgtgg agtaccttta atgaaatcta tccagttgca accaatcaat
    17041 atttgttagg tgttttccca ccaggaatca aatatgattt tacaaagatc gtcgcgtgtc
    17101 ttcataatat gatggttgct catgcccgtg tagtgaacta tttcaaagaa aacgaacttc
    17161 cgggtgaaat tggtgtagtt cactcattag agacgaaata tgcggcaaca gacgcgccgg
    17221 aagataagca tgctgccttt ttagacgatg cattgtcgat ccgtttctta ttagatgcta
    17281 cctatttagg ctattattca actgaaacct tgactgctct tgatgagata tgtgaagcaa
    17341 atcaggccag ctatcatttt cctgaagaag atttcgttga attgaaaaaa gcatccacgc
    17401 gtaatgacta cttagggatc aatcactacc aatgccattt tgtcaaagct tacgatggag
    17461 aaaatgcaat ccatcataac ggtacgggtg aaaaagggac ctctgtttat aaagtgaagg
    17521 gaatcggaga acgtatttat aaagaaggaa ttcctcgaac tgattgggac tggctgattt
    17581 atccagaagg tctatatgac ttgttgctgc gaattaaaag cgattaccca cattacaaca
    17641 aaatctatat cactgagaac gggatggggt ataaagatca gtttgaagat ggcattatta
    17701 tggatcagcc gagaatcgac tatttgagag tctatttgga atccttaagc aaggcgataa
    17761 cagccggggt aaatgtgaag gggtattttt tgtggtcgct tatggatctt ttctcatgga
    17821 caaatgggta taacaaaagg tatggcttgt tttatgttga ttttgaaacc cagaagcgtt
    17881 acccaaaaga atcagcttac tggtacaagt tggtcagtga aaccaaaacg attatttaat
    17941 aagtgataat ggaggaagaa aagatgaata aagaggaatt acaaatgctt ggttttgaga
    18001 tcgttgcata ttcaggcgac gctcgcagta cattattgaa attattgaaa gaagtaagac
    18061 aaggaaactt tgaacatgtt gattcggcgt tgaaagaagc cgacgaaaat ttaacccttg
    18121 cacataattc gcaaacaaaa attttagcgg aagaagcatc tgggaaagag atggagatgg
    18181 gattcatttt tatccatggg caggatcatt taatgaccac tttattgctg agagatctga
    18241 ttcaagactt tattgtactg tacagacaga atcaaggata ggagaagaac gctatggatg
    18301 gaatcattaa acaaatcgaa aaagggaaac cattttttga aaaaatctca cgtaatagtt
    18361 atttaggagc cgttcgtgac ggatttttaa cagcgatgcc agctatttta ttttcaagca
    18421 ttttcatttt gattgctgcg gttcctgaga tttttggtta ccaatggcct gaatctgtct
    18481 ccacatggct atggaaagtc tataattatt cgatgggtgt tgtagggtta ctcgtttcag
    18541 caaccactgc tcgctgcttg gctgattcga tgaatcgtcg cttaccgaaa aacaaagtaa
    18601 tcaacaccac ctctgtgatg ctggcctctg tggtaagttt cctgttgtta agcgtctctc
    18661 cgattgaagg tggattttca acggaatata tggggacaaa gggactactg gcttcctttg
    18721 tcgctgcatt tttaacagtt aatttatata agttttgtgt gctgaaagat attaccatta
    18781 aaatgcctaa agaagttcct ggaacgattt cgcaaacctt ccgagatatt tttcctttct
    18841 ctttttcagt atttgcggcc gttttaattg atttaggcat tcgacaaatc ttccaaatct
    18901 catttgcaga agcaatcatt acggtgctgc agccgctatt tacagcagct gacgggtact
    18961 taggaatcgc tattatctgg ggagcgatgg cgctgttttg gtttgtcggt gttcatggac
    19021 cgtcgatcgt ggagccggca attgcggcaa ttatctatgc caatgtggaa accaatctgc
    19081 aattgttcaa ttccggtgaa caagcttcta aagtactgac ggtgggttta gggaattttg
    19141 taggaactat gggaggaact ggggcaacat tggttgttcc ttttatcttc ctactctttg
    19201 ctaagtctaa gcagttgaag gcagttggga aagcttcctt tattccagta agtttcgctg
    19261 tcaacgaacc attgctattt gcggcaccca tgattttgaa cccttatttt tttattccat
    19321 ttttgttaac acctgttgta aacgtttgcc tgttcaagtt ttttgtcgat gtattgggca
    19381 tgaacagctt tatgtacgtt ttgccttggg caactccagc accgatcggt ttggttctag
    19441 gaacgggcat gggtatctta gcttttgttc tagcagcggt tctaatcgtc gttgacttcc
    19501 tgatctatct accattttgc aaagcatacg atgctgtctt agtagagaaa gaagcacaaa
    19561 aagcagaggt gatcgcaacc gaggaaggtg cttctgccga tgtacgtgag gaagtacctg
    19621 tggcagcgag tcaagttatc agcgatgcaa ataaagaaat caatgtgttg gtactttgtg
    19681 ctggcgcagg gacaagtgcg atgttagcta atgctttaaa aaaaggtgca tctgaatcga
    19741 aagcaccgat cagtgcaatg gcgggtgctt atggttctca ttacgaaatg ctgcaggatt
    19801 ttgatatggt ggtcttagca ccgcaagtca attcttacta tgatgatatc aaagaagaca
    19861 ccgatcgctt aggaatcaag ctggttgcga caaaaggagc agaatatatc ggattaacga
    19921 gagatcctga aaaagctgtt cagtttattc tgaaccaatt tcaataaagg aggagccata
    19981 atgaaaatgg gaatttggtt gcgtaaagct gttttatttg cagctgtgtt aggggctttg
    20041 ggggctgttt caaaaactgt tgaagcatct gccagcgaat ttattcgcgg agcagatatt
    20101 tcgatcctgt cagatatgga aaaatctggt gcaaaatatt atgaagacga tatttcaaaa
    20161 gatgccttag agattttaaa aaataatggt gtcaactatg ttcgactgcg tttgtggcag
    20221 gatccttata atagtaatgg agaaagttat ggagcaggga caaatgatct ggaaacgacg
    20281 attgaccttg caaaacgagc aaaaaatctg ggacttaagg tgctgctgga ttttcactac
    20341 agtgattttt gggttgatcc tgggaaacag aatcttccta aagcatggca aggtctgacc
    20401 tttgaagaaa tgaatacggc attgtacgac tatacaaaga acgttctgtc ggaaatgaag
    20461 cagctggacg tttatcctga tatggttcag atcggcaatg aactgaatag cggaatgctc
    20521 tggccttatg gcaagtcttg gggagaaggc gggggagaat ttgatcgatt ggctgctttt
    20581 ttgaagtctg gtatccaagc tgttcgagac actcaaccta aaacaactcc agtaatgctc
    20641 cacttagctg atggtggcga tacaggagca tttacttggt ggtttgatga aatcacgagt
    20701 cgaggtgtgt catttgattt aattggagtg tcgtattatc cgtattggca cggttcaatg
    20761 aatgaacttc aaaccaatat gaatgcgatc agccaacggt atggtaaaga agtgatcgta
    20821 gttgaaacag cttatgggca tacaacagcg aatgccgata ctatgccaaa tgcttttggt
    20881 gaagccgaag cagctgcggg gggatatgaa ccaacaccga caggacaagc agagtatttg
    20941 ctggatcttg ccgatcggat tcaggcagta ccgaataatc gcggggcggg cttcttctat
    21001 tgggaacctc tttggtacaa cggaaatgtg agttgggcaa cacaagcagg tatgaattat
    21061 ctcagtgttc aatccactat gggaaacgaa tgggacaacc aagcgatgtt tgattttgct
    21121 ggaaacgcct tgccaagctt gcgtgcgttt aaacaagcag gggcgcaaat aaatctagtt
    21181 aaaaatgcaa gtttcgaaag cgatggacca acgaccagtc cttccaattg gcaaacctgg
    21241 tttcaacaag gaaccgctaa gggtacgatc aaaacggaag cgggcacttt ttttggggac
    21301 tacaaactaa ctttttggca ggatcaagca tacgaggcat cagcttatca aaatgttagt
    21361 ggcataagca aaggaactta cacattgagt gcctatgtaa tgtctggggg agagtttgat
    21421 actaatcagt tatatatcaa aaattatggt ggagcggaac taaatactgc ggttgttaaa
    21481 acaagtgaat ggacaaaaat tacaatcaaa gacattccaa ttacaacagg tgtttgtgaa
    21541 attggggttt atagcaaggc ttcaggaaat gcttggacga acgttgattt tgtttcattg
    21601 gtgaaacaat agactgcgga tcgtttcgat tcaagatcgg aggttccctt cagccacatc
    21661 gttagcgtag aaggcaggaa tcgaacctgc acctcgcctt tgccaaaaat aaccagaaac
    21721 gatttccagt catttttacc aaaacgagta aagcaccaga cttcatctac actttgtatt
    21781 atgctaaaga gagttcaacg atttcgtaag aagcaatgaa aaaattgcaa aaagttgtgc
    21841 agataacata aagtttgttt aagaaagggt aaccttagaa ttccgtcgtg aatatgattt
    21901 gagcacttag atggatagaa aaaagttctc ggaaaacagt tcgtttttcg agaacttctt
    21961 tttatgataa gaatgaaaca aattaactta tattatagtc tcttttggta gggaaccatc
    22021 ttatttttaa tgaaaatcaa gcattttagc tattgaagaa tgttgattta tagggattcc
    22081 ccctccatat tatgttggct ctttattccc actctgtttt caacgtaaag tgtataaaac
    22141 agaatgcgat actatcaagg gagttaaaac aatatacaga acagtaaaac actataaata
    22201 aaaataaaag tgcaataaaa gtgcaatttt tgcacctaat catactctgt ttatatatga
    22261 aaactagatt ctgaatccaa tatttggtta acgagaggcg ttcgaatttg aacgtctttt
    22321 atatattact ttcaaataaa tatgaggtag aatactatct attttctttt taacccaatt
    22381 gttataatgg agagtgtaaa atattttgtg taaatagaaa aaaggaagtc ccttctgtag
    22441 aatagagtta ccacaacaca ttcacagaag agaggacttc cctatgaacg attttactac
    22501 agaaattcta aagactctag cgaacaaagg cgatttgaat gaattattcc gtgtccattt
    22561 ggagaaagca gtcaatacgc ttctcaaaac ggagttaacg gctttcctag attacgaaaa
    22621 gtatgatcgc attggtttta acacgggtaa ttctcgtaac ggctcctatg accgtacggt
    22681 caagaccgag tatggggaac ttcatctcca gattccgcgc gaccgcaatg gtgagttcaa
    22741 gcaacagact gttcctgctt atagacggac gaatgacacg ttagaggaga ccgtcattca
    22801 cctcttccga aaaggtatta ccatgtcgga aatcgcagac ttgattgaga aaatgtatgg
    22861 gcattactac acgccccaaa ccatgtccaa tataacaaaa tcatttacag aagaggtaac
    22921 ggcgtttaat gggcgggagc ttcatgaccg ttatgctgct atttatatgg acgcaacgta
    22981 tattccgtta aagcggaaaa ccgtcgccaa ggaagctatt catatcgcag ttggcattcg
    23041 cccggacgga tcaaaggaag tattgagcta tgcgattgca ccgactgaat ccatcacgat
    23101 ttgggaggaa attttattgg accttcaaga gcgcggtttg aaaaatgtcc tcctgttcat
    23161 cacggatggc ttaaagggga tggtaggagc gattagtcgg ttctatccca aagctcgttt
    23221 tcaacattgt tgtgtacacg tttcccgtaa tatcagtcat aaagtgcgtg tcgatgatcg
    23281 taaggaagtc tgtgatgatt ttaaaatggt gtatcaagcc tcatctaaag aggtggcgtt
    23341 ggaagcacgt ggtacttttg cggaaaaatg gaaaaccagc tatccaaaag tggttgaatc
    23401 gattctttcg aacgatcact tgctcacttt ctatgatttc cccttggcca tacgcaagag
    23461 tatttattct acgaacttga ttgaatcctt
//
LOCUS       DACGLE010000040        23118 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0534
            SAMEA3304449-rid9560453.denovo.041, whole genome shotgun sequence.
ACCESSION   DACGLE010000040 DACGLE010000000
VERSION     DACGLE010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304449
            Sequence Read Archive: ERR985064
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23118)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23118)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702225.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702225.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 20:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,045
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 27 of 67
            Pseudo Genes (incomplete)         :: 25 of 67
            Pseudo Genes (internal stop)      :: 19 of 67
            Pseudo Genes (multiple problems)  :: 4 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23118
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304449-rid9560453.denovo.041"
                     /strain="VREN0534"
                     /db_xref="taxon:1352"
     gene            complement(61..1437)
                     /locus_tag="IXT20_001671"
     CDS             complement(61..1437)
                     /locus_tag="IXT20_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ4860118.1"
                     /translation="MDLFRKKEINLNHTSEMKKELKTSDLIMLGIGAIIGTGIFVVTG
                     VAANQNAGPALSLSFVLAAIVVILSGLSFAEFASRVPVIGGPYAYLYVVFGEFAAWLT
                     GWLLIGEFLLAVSSVASGWSGYMQGFLKSLGAELPQALTGGYNPENGTYIDLIAVLVV
                     IFVTYIVSLEAKKALRLNNAMVYVKFGIIALFIIVGIFFVKPDNWQPFMPFGFSGVLD
                     GAALVFFAFLGFDAVAMAAEEVKNPQKDVPRGIIGSILIATVLYIIVTLILTGIVPYT
                     ELGVNDPVAFAMRYVGHGTVGAVIAVGAILTLLTVTISMMYSLARLLFAVSKDGLFPK
                     FMTEIDQKHRTPKKATYAAGVAAVFFAGFFPLNVLAELTNIMALAYLMLVSLGLLKLR
                     KMFGKPKAGEFKVPFVPLLPIVSILTCIVLMLRLQTVTWIVFGIVMVIGLIIYFGYGY
                     GHSKMNEK"
     gene            complement(1474..2340)
                     /locus_tag="IXT20_001672"
     CDS             complement(1474..2340)
                     /locus_tag="IXT20_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose transporter GlcU"
                     /protein_id="HAQ4860119.1"
                     /translation="MEILIALVPMLAWGSIGLVSGKIGGDANQQTLGMTIGAFLFSLV
                     VFFVVSPVITPWIFLIGFLSGLAWSVGQNGQFHGMKYMGVSVGLPLSTGFQLILNTIA
                     GAVFFHEWTQTKDYVYGIIALALLVSGAYLTARQDDEGKIDTDNKMLDFGKGFRALIF
                     STIGYGVYTIIVNWANLDAMSIILPQSIGMILGASFFAFRKVKVDQFVWKNMICGLLW
                     GLGNICMLLTVKSLGLAVGFSLSQMGIIISTLGGIFILGERKTKKELIYVIVGCLLVI
                     LGGILLGYMKTV"
     gene            complement(2429..3454)
                     /locus_tag="IXT20_001673"
     CDS             complement(2429..3454)
                     /locus_tag="IXT20_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAQ4860120.1"
                     /translation="MNFFKYLKDQWTLILGWLFFIALTIFVMWLAPNIKVDLSTIAYL
                     ALIEGVFLIFYLLGGYLSKRRWWGKLADIQKTSPLQNYLSGGRTEEEKLVEEYINQLI
                     REHQEVMQSAISNQQDQKDYIDSWVHEIKVPLSASKLLVRSIEFDIDDQKYTLLENEL
                     SKIDEYVEQVLYYARLDSFSRDYLIQEYGLKGIIQPVIRSQANYFIQKNIRYEIVGED
                     HTVLTDAKWIAFIFNQLLSNAIKYTPDHGNIIVSIEKEAQGVSLSVKDSGIGIPAEDL
                     KRIFDKGFTGRNGRLSKTHSTGLGLYLAKNLAEKLGIHLTAESTEGKGTTMTLFFPIL
                     NFYNEKR"
     gene            complement(3472..4146)
                     /locus_tag="IXT20_001674"
     CDS             complement(3472..4146)
                     /locus_tag="IXT20_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ4860121.1"
                     /translation="MAKIMVVEDEEIIRQLIMEELEKWQFETFGTTDFNQVFSDFERE
                     EPQLVLLDINLPVLDGYYWCQKIREVSKVPIIFISSRNTNMDMIMAMNMGADDFVTKH
                     FQIDVLIAKINALLRRSYNYTELSSEMMSHNGITLNVDNGSMEINGEIIDLSKNEYRL
                     LFILMKHNGKILSREKLLRALWDDERFVDDNTLTVNINRLRRKIEQAGIQGYIETKVG
                     QGYIVP"
     gene            complement(4330..5223)
                     /locus_tag="IXT20_001675"
     CDS             complement(4330..5223)
                     /locus_tag="IXT20_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAQ4860122.1"
                     /translation="MKKKIGNRPQTQISLLLQLFMAVMFLAGAAVFTYPFLADALSNY
                     LDQRRIENYQKQLERDKEEKHEQQLAVHEKKNQTLAHTSVIPGMGQVKDPFEQAVRNV
                     QNPGKEYYEQHMIGAIYIPKINVSLPLFDETNDLLLDRGATVLQGTSFPTGGESTHSV
                     ITAHSGIAEKKLFTDLEEMEQDDRFYLEVYGRMLAYEVVEKIVVLPTKTNTLAIREKQ
                     DLVTLITCTPYTVNTHRLLVTGRRVPFTEEVSSKIEQTKKYHLYRLLILLLGILLVLT
                     LFGYWCYRKFKRQKQRKKNNR"
     gene            complement(5297..6994)
                     /locus_tag="IXT20_001676"
     CDS             complement(5297..6994)
                     /locus_tag="IXT20_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HAQ4860123.1"
                     /translation="MNKKMIVTGMSVLLLVTAGIGIFGNGKLVKAEEVAQKPSEVTIT
                     LHKKGFSSVPEERPNSGLVSTDFGEENIPGVDFDLFDVTEVYYDLIRDNPLTPEREDG
                     LNSAEAIEWIQKRHTESWFLKYRLTSIDKQTTNEAGEAVFSTVQVTEEAPSSRDKVYL
                     FLETYSPAHISRIASPMVVMMPVMMPDMVDGVWDGSTWKDTYNTDVHLYPKNEIREAD
                     KQMNVEESDLRQVTIINEAGEQETISYIDLERGKTASYTITAPIPYFIDSVLENGSAV
                     IKNYKITDTPTVGLTYYDQEIEVRAGETILTKGQDYIVEVVSNGFVVTILTEENGVAK
                     VDTLGRLADARGGDLTITYNLKVSTELEADDFHNNTAVIEIGRNDEFDYEEGVEPPEK
                     VTTGGRKFEKYDASSSELLKDARFELWNEDRSEYAIFYKGESPLAVYESGADRIEWAT
                     SGQATEFVADGNGYFEVQGLDYGTYQMKETMAPEGYVLPTGEAAFTEFIISYGSYNEE
                     IQIVGVENPGPERVPNMKRGSLPATGGNGLLAFLLIGISLMIGAYSWYRKSKMKSEV"
     gene            complement(6987..8300)
                     /locus_tag="IXT20_001677"
     CDS             complement(6987..8300)
                     /locus_tag="IXT20_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HAQ4860124.1"
                     /translation="MKKLLCLMLQLLVLLPLAAVLLPHAMSAEESGEDVVEFVLHKRM
                     IRDIDYNDQFEYHENDGLAISEEAGETADIISQTVPLNGATFAIYDMTDYYHEKKAAE
                     NMTPKEFVQQIAETNRNNIRQLITEERLVQIGTSVTTGKDTVGGLGDGIARIKAPKKN
                     QDRDAVYLIFEESIDPEVGLDVDIDQTAIPIAAILPIYHPTESTEELQEIHIYPKNVG
                     YLRDPYFFKYGRQRGTTEKGKPLEGAVFALYQMIEGGKYYLDMAPANDLKNQWIKPAD
                     NDPLNDKNVSKFISDKEGLVTTGQRFLPSGTYYFEELKGVEGYEMDEASKRIEVIIPE
                     FWVDAKGQPQYVVVNGQEMAELESGKVPVSAYESATPRVYNETVKETTDKPKEPSGPS
                     NPGQSGKWSVFPQTNEARTLISLLGILLLLGAVLLIKKERGEKNE"
     gene            complement(8300..12337)
                     /locus_tag="IXT20_001678"
     CDS             complement(8300..12337)
                     /locus_tag="IXT20_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HAQ4860125.1"
                     /translation="MVVDERIDNQELQENETDTEDSAEIQRSEENEMEKLDEPIVPSG
                     QALQRIQRQLFNGILERLGPTATQDPYEYIYPSGEVENEQHRYPKFTTNDYTKVLGNW
                     TQSNERYQGDKGSPYDGENLIDDSDYTNGGANWRNYDYATNNGSTAGEEDRNVPAQTV
                     QLWGNERNFENSYLDYNGAYIKKWVEPVLPSSTASDLHPEDATTLYNVYLDVIGGEKK
                     EISPIDIVFVLDKSASMSELTAGTNSQTKNAALIEAVNEMSKDLLSDPSLDIRIGMVN
                     FYHNSTAINNHEQISSDIFPLTNDINRLTGSENTALNRTPIGGTPLTLGLKNGYETLY
                     KDNGGENRNPEKILIVVGDGTPTFSYAPIQSSYRTSTNGAWSNWTVMEDKIAEDNDVL
                     FRNFEEFSGNTSNAGFTHPVTYASDFNRPEDEVNVHYRYGEVKEGDNKATHWVGDGSS
                     NNNTNGSPTSQEKSSAINTVAYHHWLKNKYQENPPSIFSIGLGIDGNVSGRQRLDAIG
                     RNVLKNIADLEEDGVTPRYYNANNKNDIVTALEDISSTFKRTIQQATLYDETGFNVSP
                     FGAGRSADIQFYHLDPGENGMKYQEPEIWDEERHGQAPPEVVATPTTYVGRENQAYKF
                     SPISLGEGEMVRIKYQVKLDAGAQDGNFCTVNNMAYIQNLEDYENDITTGRMYFPSSS
                     IRYEHKGRNFQVSKKGQNGEPLAGVEFALYDQNPESGEAEPIETSKTTTDGIAIFETK
                     MPILGDETGAVSDIFWIREIAGPPRYQLVDETYQFQIKKYAANEDEMLGHYELVDEYT
                     SGSRVGGPEGKDPNSYYEGEYHKLSVVQEPNIEGTEYDELYVKLEMRNEFKPIHMNIN
                     KLIEGSNMPINGAEFELYETDGNGLEIGYPVAKGLSGVNEVEEEKGRLTFYSVDEDGK
                     YVIVDGEKVIHPIGEPDSFVYKDSEETTNYAEYKIIESQNPEGFKEPDENDTWILRLY
                     DNAIGTIQLKQTGEENWQYLTYTTDEEDRLWVAFTAYNTFDYREIRVRKLDHEGHTLD
                     GAGFDIRKVGDSSFPLYRAYTGNPLEPEESKRLPGIGYFYSRAVGVEKYVDLNYDQPL
                     HLSIGEYEVSESVAPNGYRLSDQVFQFALNEEGKFIIEDTVIEDETAPLPEGYEIIDG
                     VLQVTLEDELAPIDLELLKIDSMNSRRLEGAEFSIEKQNSAGEYEIIEGGLAPDPNDS
                     SLFNASDLLEGVYRIKEVKSPDSYRKLPGYFILEISYREEPDIENDRVVPGKEAGTLK
                     VEISYYPNEEAETPDLQQELEYELLETNRIRLQLSVGNDPENPLPATGGSGRLFYILL
                     AAFFMALAGAAYGLFVRQSKKEGAR"
     gene            complement(12350..12808)
                     /locus_tag="IXT20_001679"
     CDS             complement(12350..12808)
                     /locus_tag="IXT20_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAQ4860126.1"
                     /translation="MKGLKLKLLQLVTVSLLFGPYFQSVYHMTTLDVAAESTETKEDE
                     VFLDEDYAKIEGKYIEKEETLEWHLAFEKKESANEGRIRLAIDAAAAGIGTVKNVRGT
                     GLSSYDEDGEDLKVETIDGQEWYVGKVYSKEAETGTLIFETEKIPDVNEG"
     gene            complement(13106..13624)
                     /locus_tag="IXT20_001680"
     CDS             complement(13106..13624)
                     /locus_tag="IXT20_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HAQ4860127.1"
                     /translation="MLRLIAKIDDSIINKFAKQRKTDSRKINFLKFISNISNGGFIWL
                     LLAAVLVLIPDTREYGLAILIGSLIYFFVGLLFIKKTTRRKRPYEKNRRLVLYTSKPK
                     TSSFPSGHTALAFLSVAALFELSPVAGILAFLFACLIAYSRIFLLLHYPTDILGGAIL
                     GSVIGILCAAVI"
     gene            complement(13626..14243)
                     /locus_tag="IXT20_001681"
     CDS             complement(13626..14243)
                     /locus_tag="IXT20_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HAQ4860128.1"
                     /translation="MMNRSIGKKANDYFLEIIEWIPRLIHLQLLWFLCVLPVFTLGTA
                     SRTVLYMIYHYKKNEEKKLGSLFWKKFRENFSLYGKQDSLASVYFLLLLIDYQIFRYW
                     GGGIGYTLMYTTLFLLLLSALLFSYKILLQLEQKEASWITAFFLFFNDFKSALFYLAG
                     TLILLVAFWFAGPIYFALLGFSFLFYFQVLLFIHRTQEKVLKKEE"
     gene            complement(14240..15103)
                     /locus_tag="IXT20_001682"
     CDS             complement(14240..15103)
                     /locus_tag="IXT20_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAQ4860129.1"
                     /translation="MTANDGLFQKDQCESANKKILSYVLMTVIGIILIIPLLWMVFTS
                     LKPMEEIVRYPPTFFPEKIVWENYLDTIAAFPFWRYARNTLFITVLVVIGNVLSNSFI
                     AYGFAKLDFPGKKLMFALVLSTMMIPGFVTMIPQYVLFSKIGWVGTYLPLIVPSFFGN
                     AFNIFLMRQFYLSINNELIEAAEIDGANHLYIWSHLMLPLTKPALITIAINSFNAAWN
                     DFLGPLLYIQDQEKYTLQIGLQVFQNQATTQWNYLMAGATLVLIPTILLFFFAQRYFI
                     EGMDLTGGSKG"
     gene            complement(15090..16001)
                     /locus_tag="IXT20_001683"
     CDS             complement(15090..16001)
                     /locus_tag="IXT20_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ4860130.1"
                     /translation="MKRLSIRKKKEARWAYLFISPFIIGFLVFMLGPMLFSVIGSFTD
                     YNLTSKMNFIGLANFKRMFVHDDLFWKSLYNTVYFVIFNVPLTTIFSVFLATVLNQKI
                     KGISVFRTAFYLPAVLSGVAVYILWMQLLSPSAGLINTFLGWFHIPGPAWLFDPQWTK
                     PALILMKIWSSGGAMLLYLATLQNVPRSLYESAEIDGAGVWGKFKSITLPLITPIIFY
                     DVITSTIGSFQIFQEAYVMTKNGTGGPANSLLFYNLHMWNKAFVAFDMGYAMAMSMIL
                     FLIVLVLTFVNLKLAPKWVYYSGGDGK"
     gene            complement(16042..17376)
                     /locus_tag="IXT20_001684"
     CDS             complement(16042..17376)
                     /locus_tag="IXT20_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAQ4860131.1"
                     /translation="MKKLLFTGLLASSALFLLGGCGSSNASDASSSAANSSEKTSDGR
                     KNIDFWSFWGSGARKEAIEEIIEDFNQSQNEIHVNYKYQPWGDIWTKSLSAITAGNAP
                     DVIVQDINSVAQRAEAQQATNLSKYVEKDTSKDFYPQLWDTVEYEGDPYAIPFNTDTQ
                     VIFYNKKIFKEAGISEQDLPTTWDELEETARKLDKKEGKEFTRIGFYPLWNLGADVWA
                     LNADNGTSWFDKDEKVKIDTKNKVEALEWIQDWQDYYGKNTINKLEAEFGSGVSDPFI
                     SGLVAMRAQNINYYSSLMENAPDDFDFGVMQLPEKEEGSGHWSWGGGFVLEVPYGAKD
                     PEASYEFIKYLSSPEVQEKFGEKSFDIMASKTANENLVNNDNLTDKGKMIYQMADENF
                     KNTVITPVPLDAPDYTTLVNEQIDQILLGTKSPKEGLADAQKAVENLVKQNQ"
     gene            17584..18414
                     /locus_tag="IXT20_001685"
     CDS             17584..18414
                     /locus_tag="IXT20_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HAQ4860132.1"
                     /translation="MFRHQKELQFEAKPDRPNPLIAKYLQELIGGQYGEMSVAMQYLF
                     QGWSCRGEEKYKDMLMDIATEEIGHVEMLATMVARLLEGAPVEDQEAAMQKDPTIGAI
                     ISGMNPQHAIVAGLGPRPADSVGNPWSGAYIIASGNLLADFRANLNAESQGRLQVARI
                     YEMIDDRGVKDMLSVLLARDSYHQNLWECAVKELEEKEGSILVPSTFPRDKERKDIAY
                     DFYNFSEGDQSSEGSWASGKALDGEGEYQWLKEPKIEGAAPKLEPVTPKMYGTPPFKK
                     "
     gene            complement(18658..18907)
                     /locus_tag="IXT20_001686"
                     /pseudo
     CDS             complement(18658..18907)
                     /locus_tag="IXT20_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005816675.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(19098..19292)
                     /locus_tag="IXT20_001687"
     CDS             complement(19098..19292)
                     /locus_tag="IXT20_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4860133.1"
                     /translation="MKTTLVLFYKKHPYFTLLINILLASVIGISVEYLINKDFIGSCF
                     YTALFLGLLEAFSIYKKSKK"
     gene            complement(19797..19982)
                     /locus_tag="IXT20_001688"
     CDS             complement(19797..19982)
                     /locus_tag="IXT20_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4860134.1"
                     /translation="MSKNSKGFLTILLAFIGYMLVGLLKSYSNELLNFSTFINDTLVP
                     SLFFIVFFAVGYFIIKI"
     gene            complement(20832..21173)
                     /locus_tag="IXT20_001689"
     CDS             complement(20832..21173)
                     /locus_tag="IXT20_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HAQ4860135.1"
                     /translation="MSEERIYIPKKGDIVWIDFDPSVGKEIQKRRPGLVVSRYEFNRK
                     TMFSVICPITSTIKNLPTRYSLPEELDIKGQVIITQLKSLDFKERKLKKIENLPLQDM
                     AKIDQIIQYMF"
     gene            complement(21173..21403)
                     /locus_tag="IXT20_001690"
     CDS             complement(21173..21403)
                     /locus_tag="IXT20_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016178973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAQ4860136.1"
                     /translation="MLVTKSRLQGSSVVVTLPSDNGKKPSENQEYIVVYSDDGTITLV
                     PKIEDPFSGGEEAEYYEKDEWEDLTPEGREIL"
     gene            21690..22028
                     /locus_tag="IXT20_001691"
     CDS             21690..22028
                     /locus_tag="IXT20_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4860137.1"
                     /translation="MPDYKQKAGYSFCSDKFQLGKFTKLAGEEVSTVRIKECPLQIET
                     LVQRISTRQDFAIVEREIVAIFVEEGILHDPTHINVDKWQPLIYKFREYTTANQSLTL
                     NFRFQEFKES"
     gene            complement(22233..22472)
                     /locus_tag="IXT20_001692"
     CDS             complement(22233..22472)
                     /locus_tag="IXT20_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HAQ4860138.1"
                     /translation="MFSNKEKIVYIGGFYRSHQDEKMAESIIMTTEPNRTVAPIHDRM
                     PLVLTEEQIEPWVTDISFARKIITQQMPELVMEKV"
     gene            complement(22448..22795)
                     /locus_tag="IXT20_001693"
     CDS             complement(22448..22795)
                     /locus_tag="IXT20_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF159 family protein"
                     /protein_id="HAQ4860139.1"
                     /translation="MCGRYFFDLESELLQNYYREAQTKQPKQAAELAAGEVFPSNLVV
                     TLRKEEENILTPEIMKWGFTGFKKGQLMINARAETVEEKKTFHQPFLQNRCVFPMSGM
                     KKKTNLCFQIRKK"
BASE COUNT         6817 a         4944 c         3898 g         7459 t
ORIGIN      
        1 gtttattaca actgttgatg ggcgtttttc ttatctttct tctaacttct ccattggagt
       61 ctatttctca ttcatcttgc tgtgtccata gccatagcca aaataaatga tcagtccaat
      121 aaccattacg ataccgaaca cgatccatgt cacagtttgc agacgaagca tcaatacaat
      181 acaagtcaag atggagacga ttggcaatag cggaacgaat ggcactttga attctccagc
      241 ttttggtttg ccaaacattt ttctcaactt cagtaagcct agactaacaa gcatcaaata
      301 ggcgagcgcc atgatattcg ttagttccgc taatacattc aatgggaaaa atccggcaaa
      361 gaatacagct gccacaccag cggcataagt cgcttttttc ggtgtacgat gcttttgatc
      421 gatttctgtc ataaacttag gaaataagcc atccttgctg acagcaaaca gtaaacgagc
      481 aagtgaatac atcatcgaga tcgttaccgt cagtagcgtt aaaattgccc ctacagcgat
      541 caccgcgccc actgttccat gtcctacata cctcattgca aaagccactg ggtcattgac
      601 tcctagttca gtatacggaa cgattcctgt caagatcagc gtcacgatga tgtaaaggac
      661 agtagcaatc agtatcgaac caatgattcc tcttggcaca tctttttgcg ggtttttgac
      721 ttcctcggct gccattgcaa cggcatcaaa tcccaagaaa gcaaaaaaca caagagctgc
      781 accatctagc acaccggaaa aaccaaaagg catgaatggc tgccagttat caggtttaac
      841 gaaaaatatt cctacaatga taaacaacgc gatgatacca aatttgacat ataccatggc
      901 attattcaaa cgtaatgctt tcttggcttc aagcgacacg atgtaagtga cgaatatcac
      961 tacgagcacc gcaatcaagt caatgtatgt accattttcc ggattgtacc cgccagttaa
     1021 tgcttgtgga agttcggccc ctaaactttt caaaaaacct tgcatatagc cagaccatcc
     1081 ggaagcaaca gaggaaaccg ctagtaaaaa ttccccgatc aacaaccacc ctgtcagcca
     1141 ggcagcaaat tcaccaaata caacatacag ataggcatat ggcccaccaa tcactggcac
     1201 tcgcgaagca aattctgcga aacttagtcc agacaaaatg acaacgattg cagctaagac
     1261 aaatgaaagt gacagtgctg gaccagcatt ttgattggcc gcaacccctg tcaccacaaa
     1321 tattcccgta ccgatgatcg caccaattcc aagcatgatc aaatcagacg tttttagttc
     1381 ttttttcatc tcactcgtat gattgagatt gatctctttc ttacgaaaca aatccattga
     1441 agttcctccc cattttaggt tggaaaacag cgattaaact gttttcatat accctaaaag
     1501 gataccacct aaaatgacta aaaggcaacc aacaataaca taaatgagct cttttttcgt
     1561 ttttctttcg ccaaggatga aaatcccacc taaagtagag ataatgatcc ccatttgtga
     1621 aagtgagaag ccaacagcta aaccaagtga tttgactgtt agaagcatgc agatgtttcc
     1681 taagccccat aataatccac agatcatgtt tttccacaca aactgatcga ctttaacttt
     1741 acggaaagca aagaagcttg cgcctaagat cattccgata ctttgaggta agataattga
     1801 catcgcatcc aaattcgccc agttgacgat gatggtatat acaccataac caatcgtaga
     1861 gaagatcaat gctctaaaac ccttaccaaa atcaagcatt ttattatctg tatctatttt
     1921 tccttcgtca tcttgtcggg cagtcaaata cgcaccgctt acaagaagag ctaaagcaat
     1981 aataccgtat acataatctt tcgtttgtgt ccattcatgg aagaaaacgg ctcccgcaat
     2041 cgtattcaaa attaattgaa aaccagtaga caatggtagc cctacagaaa cacccatgta
     2101 cttcattcca tggaactgtc cattttgtcc aacactccaa gctaagccgg ataaaaatcc
     2161 gatcaagaaa atccaagggg tgattacagg actaacaacg aaaaagacaa ctaaagaaaa
     2221 cagaaatgca ccgatcgtca ttcctagtgt ttgttgattg gcatcgccac caattttacc
     2281 acttactaaa ccaatactac cccatgccaa catagggact aatgctatta aaatttccat
     2341 aaccaatctc cttttatttc acatgataag aaacttatca gcgaaaacgt taacaacaca
     2401 agcacttttt ttcgtttcta acgattttct aacgtttttc attataaaaa tttaagattg
     2461 ggaaaaataa agtcattgtt gtcccttttc cttcagttga ttcagccgtc aaatggatac
     2521 ctaatttttc ggccagattt tttgctaaat aaagtcctaa tcctgttgaa tgtgttttgc
     2581 taagtcgacc atttctccca gtaaatccct tatcaaagat ccgtttcaag tcttccgctg
     2641 ggataccgat ccctgaatct ttaactgaaa ggctgactcc ttgcgcttcc ttttctatgc
     2701 tcacaatgat gtttccgtga tccggcgtat atttgatggc attactaagc aattggttga
     2761 agataaaagc gatccattta gcatcggtca aaacagtatg atcctcacca acgatctcgt
     2821 agcgaatatt tttttggata aaataatttg cctgactgcg tatcacaggc tgaatgattc
     2881 cttttagtcc atattcttgg atgaggtagt cccgcgagaa actgtcgagt cttgcataat
     2941 acaaaacttg ttctacgtat tcatcgattt ttgataattc attttctagt aatgtatact
     3001 tttgatcgtc aatatcgaac tcgatcgaac gaactagcaa tttagaagcg gaaagtggca
     3061 ctttgatttc atggacccat gagtcaatat aatctttttg gtcttgttga ttactgattg
     3121 ctgattgcat tacttcttgg tgttcacgga tcagctgatt gatatattct tctaccagtt
     3181 tttcttcttc tgtcctccca ccactcaaat aattttgcaa aggggaagtt ttttgtatgt
     3241 cggctaattt tccccaccat cttctcttgc tcaagtaacc acccagcaga taaaaaatca
     3301 gaaagacgcc ctcaatcaaa gctaaataag caattgtgct caaatcaact ttgatgtttg
     3361 gcgccagcca catcacaaag atcgtcaatg caataaaaaa cagccatccc aagatcaatg
     3421 tccactgatc tttcaaatat ttaaaaaaat tcatcttaac cgctcatcct ttcagggaac
     3481 gatatatccc tgtcctactt ttgtttcgat atatccttgg atacctgcct gttcgatttt
     3541 tcggcgtaac cgattgatgt tgactgtcaa tgtattgtca tctacaaatc gttcgtcatc
     3601 ccacagtgct cgcagcaatt tttcccgact taagatcttt ccattgtgct tcatcaaaat
     3661 aaataataga cgatattcat ttttgctcaa atcaatgatc tctccgttga tttccatact
     3721 tccgttgtca acattcaacg taatgccatt gtggctcatc atttcactag ataattctgt
     3781 gtagttgtaa gaacggcgaa gtaatgcatt gatttttgca atcagtacat cgatttggaa
     3841 atgttttgtc acaaaatcat ctgcccccat gttcatagcc atgatcatat ccatattcgt
     3901 attcctgctg gagataaaaa tgatcggtac tttcgatact tcacgtattt tttgacacca
     3961 ataatagcca tccaaaacgg gaagattaat atctagtaat acaagctgtg gttcttctct
     4021 ttcaaagtct gagaacactt gattgaaatc ggtcgttcca aaggtttcaa attgccattt
     4081 ctcaagttcc tccatgatca attgtcgaat gatctcttcg tcttcgacta ccatgatttt
     4141 tgccataaaa aatccctcca tctttcttct tgctactaca atatcaaaga agcccttgtg
     4201 aaacaagttg gcttacctgg aaaatgtcat tttagcaaac gttaaaaaaa actggaaata
     4261 tgcgatttaa tcgcgtttgt ctcttccaag cagcttaaat ttattatgtt attcttaata
     4321 attggtctat tatctgttat ttttctttcg ttgtttttgt cttttaaatt ttcgataaca
     4381 ccaataaccg aaaagcgtta gcactagcaa tatacctaga aggagtataa gtaatcgata
     4441 aagatggtat ttttttgtct gttctatctt tgaagagact tcttctgtaa agggaactcg
     4501 tcttcctgtc actagtaatc ggtgagtgtt cacggtgtaa ggtgtacaag tgataagtgt
     4561 gactagatcc tgcttttctc taattgctaa tgtatttgtt tttgttggaa ggacaacaat
     4621 tttctcaacg acttcataag cgagcatccg accatacact tcaagataga atcggtcatc
     4681 ttgctccatt tcttcaagat ccgtaaagag ttttttctca gcaataccgc tatgggcggt
     4741 aatcacggaa tgtgtactct ctcctccagt tggaaaagag gtgccttgca ggactgtcgc
     4801 tcctctatct aacaaaagat cattcgtttc atcaaataaa ggcaagctaa cattgatttt
     4861 cggaatatag atggctccga tcatatgttg ttcgtaatat tcttttcctg gattttggac
     4921 atttctaacc gcttgctcaa aaggatcttt gacttgtccc attcctggga ttactgatgt
     4981 atgagcaagc gtctgatttt tcttttcatg cactgctaat tgttgctcat gtttttcttc
     5041 tttatcgcgt tcaagctgct tttgataatt ctcgatcctt ctttgatcca aataattgct
     5101 gagagcatct gctaaaaatg gatatgtaaa aactgcagct ccggcaagaa acatcactgc
     5161 cataaataat tgtaataaaa gactgatttg ggtttgaggt ctattaccta ttttcttttt
     5221 cattttcttc accttcttat ttaaaaaaac tgcagcaacc tttataaaac cgattgctgc
     5281 agcttatttt tgttgcttat acttcagact tcattttaga tttacggtac cagctgtacg
     5341 ctccaatcat caaacttatt ccgattagta agaatgccaa gagcccgttc ccaccagtag
     5401 caggaagtga cccgcgtttc atattcggta ctctttctgg ccctggattt tctactccta
     5461 cgatctgaat ttcttcatta tagcttccat aagagatgat aaattcagta aaggctgcct
     5521 cacctgttgg aagtacataa ccttctggcg ccatggtctc tttcatttga taggttccat
     5581 aatctaaccc ttgaacctca aagtatccat ttccatctgc gacaaattct gttgcttgtc
     5641 cgcttgttgc ccactcaatt ctatctgctc ctgattcata aacagctaaa ggcgactccc
     5701 ctttataaaa tattgcgtat tcagaacggt cttcattcca caattcgaat cttgcatctt
     5761 taagcaattc actgcttgat gcatcatatt tttcaaactt tcttccacct gttgtgactt
     5821 tctctggcgg ctctactcct tcttcataat caaattcatc atttcgtccg atttcaataa
     5881 ccgctgtgtt gttatggaaa tcatccgctt ctagttctgt ggaaaccttc aaattatacg
     5941 tgatcgttaa atctcctcca cgtgcatctg ctaatctacc taaagtatct actttggcta
     6001 ctccattttc ttctgtcaaa atcgtcacga caaacccgtt gcttactact tctactatat
     6061 aatcttgtcc ttttgttaaa atcgtctcgc cggctcgcac ttctatttcc tgatcataat
     6121 aagttagacc aactgtaggc gtatctgtga ttttgtaatt tttgataaca gcgcttccgt
     6181 tttcaaggac agaatcgatg aaataaggaa taggtgctgt gatcgtgtaa gaagccgttt
     6241 ttcctctttc tagatcaata taagaaatcg tttcttgttc tccagcttca ttaataatcg
     6301 ttacttgtct aaggtcgctt tcctctacat tcatctgttt atctgcttct ctgatttcgt
     6361 ttttcggata caaatgcaca tctgtattgt atgtgtcttt ccatgtagaa ccatcccaca
     6421 cgccatccac catatcaggc atcatcactg gcatcataac aaccattgga gacgcgattc
     6481 tggaaatatg agctggcgaa taagtttcta agaaaagata gactttatct ctagaagaag
     6541 gtgcttcttc cgtcacttgg acggtagaga ataccgcttc tccagcttca ttcgttgttt
     6601 gtttgtcaat acttgtcagt cgatatttta aaaaccaact ttctgtgtgc cgtttttgaa
     6661 tccattcaat cgcttcggca gaattaagac cgtcttctcg ttccggtgtt aaaggattat
     6721 ctcgaatcaa atcgtaatat acttccgtta cgtcaaacaa atcaaaatcg actcctggaa
     6781 tattttcttc accaaaatcc gtagaaacca atccgctatt cggtcgttct tccggaacag
     6841 atgagaaacc ttttttgtgt aatgtaatcg tcacttcact tggtttttgt gcgacttctt
     6901 cggctttgac tagttttccg ttcccgaaaa tccctattcc cgcagtaacg agtaacagta
     6961 cactcattcc cgtgacaatc atttttttat tcattttttt ctccccgttc tttttttatt
     7021 aataaaacag cacctaacaa taaaagaatt cctagtaaag aaatcaacgt tcttgcttca
     7081 ttcgtttgcg ggaatacgga ccattttcct gattgacctg gattgcttgg accactcggc
     7141 tctttcggtt tgtccgttgt ttcttttacg gtctcgttat aaacacgtgg tgttgcggat
     7201 tcataggcag agactggaac tttcccggat tctaattcag ccatctcctg tccgttaact
     7261 actacatatt gtggttggcc ttttgcatcc acccaaaatt cagggatgat gacctcgatt
     7321 ctcttacttg cttcatccat ctcgtaacct tccacacctt ttaattcttc aaaataatag
     7381 gtgccagaag gcaagaaacg ttgtcctgtc gtaacaaggc cctctttatc cgaaatgaat
     7441 ttgcttacat ttttgtcatt caaaggatca ttgtctgctg gtttgatcca ttgatttttc
     7501 aagtcattag caggagccat atctaaataa tatttccccc cttctatcat ctgatataga
     7561 gcaaaaacag caccttctaa tggttttcct ttttcagttg ttcctctttg acgtccatac
     7621 ttgaagaagt atggatctct tagataaccg acatttttag gatagatatg aatctcttgc
     7681 agttcctcgg ttgattctgt cgggtgataa attggcaaaa tagcagcaat tgggatagct
     7741 gtctgatcga tatcaacatc caatccaact tctggatcaa tcgattcttc gaaaatcaaa
     7801 taaactgcat cccgatcttg gtttttctta ggtgctttga tacgtgcaat cccgtctcct
     7861 aatccgccaa ccgtatcttt cccagttgtt accgaagtcc ctatttggac aagcctttct
     7921 tctgtgatta gctgacggat attgtttctg ttcgtttcag cgatttgctg gacgaattct
     7981 ttaggagtca tgttttcagc agcttttttt tcatgataat aatcggtcat gtcgtaaatg
     8041 gcaaatgtcg ctccatttag tggcaccgtc tgtgaaatga tatctgctgt ttcccctgct
     8101 tcctcagaga tagccaagcc atcattttca tggtattcaa attgatcgtt ataatcgata
     8161 tctcgaatca tccgtttatg caacacgaat tccacgacat cttcccctga ttcttctgcc
     8221 gacatcgcat gtggaagaag gactgctgca agcggtagta aaacaagtaa ttgaagcatc
     8281 aaacacaaca gctttttcat taccttgctc cctccttttt agattgtctg acaaacaaac
     8341 cataggctgc tcctgcaagc gccatgaaga atgcagcaag caggatatag aataatcgtc
     8401 cggatcctcc tgtagcaggc aacggatttt caggatcgtt cccgacacta agctgtaatc
     8461 tgattcgatt tgtctccaaa agttcatatt ctaactcttg ctgtagatca ggcgtctctg
     8521 cttcttcgtt tggataatac gaaatctcaa cttttagtgt acccgcttct tttcctggaa
     8581 ctacccgatc attttctatg tcaggttctt ctcgataact aatttctaaa ataaagtaac
     8641 ctggaagttt tctatagctg tcaggtgact tcacttcctt gatccgatag accccttcca
     8701 acaagtctga agcattgaat agggaagaat cgtttggatc tggtgccaag cctccctcga
     8761 tgatttcata ctctcctgca ctattttgtt tctcaattga aaattctgcg ccttcaagac
     8821 gtcggctatt catactgtca attttcaaaa gttccaaatc gattggcgct agttcatctt
     8881 ctaaggttac ttgtagtaca ccatcgatga tttcatagcc ttccggcaag ggagcagttt
     8941 cgtcttcgat gaccgtatct tcaatgataa acttaccttc ttcattcagt gcaaactgga
     9001 acacttgatc agatagccga tacccatttg gtgcgacaga ttccgagacc tcatattcac
     9061 caatagacag atgcagaggt tggtcgtaat ttaaatcgac atacttttct actcctacgg
     9121 cacgagaata gaagtatccg atcccaggca aacgtttcga ttcttctggt tctaacggat
     9181 taccagtgta tgctcgatac agcgggaagg aagaatcccc cactttcctg atatcgaaac
     9241 ctgcaccatc taatgtatgg ccttcatggt ctaattttct aactcggatc tcccgataat
     9301 caaacgtatt gtaagcagtg aatgctaccc ataaacgatc ctcttcatct gttgtatacg
     9361 tgagatattg ccaattttct tcacctgttt gcttgagttg gatagtccct atagcattat
     9421 cgtaaagtcg caagatccat gtgtcatttt catcaggttc tttgaaacct tctgggttct
     9481 ggctctcaat gattttgtat tctgcatagt tggtagtttc ttcactatcc ttataaacaa
     9541 atgaatctgg ctctccgatt ggatgaataa ctttctcacc atccacgatt acatatttcc
     9601 cgtcttcatc tactgaatag aacgtgagtc tccctttctc ttcctctact tcgttcacac
     9661 cagaaagccc tttcgctact ggatatccga tttccagtcc gttaccatct gtttcataaa
     9721 gttcaaactc tgcgccattg attggcatat tactgccttc aattagttta ttgatattca
     9781 tgtggattgg cttgaattca tttcgcattt ccagtttgac atacaactca tcatactctg
     9841 tgccttctat gttaggctct tgtacaacag aaagcttgtg atattctcct tcataatagg
     9901 aattcggatc tttaccttcc ggtccgccga ctctcgaacc agatgtatat tcatctacaa
     9961 gctcgtagtg acctaacatc tcatcttcgt tggctgcata ttttttgatt tggaattgat
    10021 acgtctcatc tactaactga taacgtggtg gtccagcgat ctctctgatc caaaatatgt
    10081 cgctgactgc tccagtttca tctcctaaaa tcggcatctt cgtttcaaag atcgctattc
    10141 cgtccgtggt agttttactt gtttcgattg gttcggcttc cccactctct ggattttggt
    10201 catataatgc aaattcgact ccagccaacg gctcgccatt ttgacccttc ttgctgactt
    10261 ggaaattacg gcctttatgt tcgtaacgaa tggaagaaga tgggaaatac atccgtccag
    10321 tagtgatatc attctcataa tcctccaggt tctgaatata ggccatattg ttcactgtgc
    10381 aaaaatttcc gtcttgagct cccgcatcaa gttttacttg gtacttgatc ctgaccattt
    10441 ccccttcacc taaagaaatc ggagaaaatt tataagcttg gttttcccgt ccgacatacg
    10501 ttgtcggagt cgccactact tctggtgggg cttgtccatg acgttcttca tcccatattt
    10561 ctggctcttg gtacttcatg ccgttttctc ctggatctag atggtaaaac tgaatatctg
    10621 ccgatctgcc agctccaaat ggtgagacat taaaaccagt ttcatcataa agtgtggctt
    10681 gctggatcgt tctcttgaag gtagaagaga tatcctccaa ggctgtaaca atgtcattct
    10741 tgttgttcgc attgtaatat cgcggtgtta cgccatcttc ttctaaatca gcaatatttt
    10801 tcaatacatt tcgtccaata gcatctagtc gttgtctccc gctaacgttt ccatcgattc
    10861 ctaaaccaat ggagaagata cttggcgggt tctcttgata cttgtttttc agccaatgat
    10921 gataagcaac tgtgttgatc gcagatgact tttcttgcga agtaggactg ccattcgtat
    10981 tgttattaga agaaccatca ccaacccagt gagtcgcttt attatctcct tcttttacct
    11041 ctccataacg atagtgtaca tttacttcat cttctggacg gttaaagtca gatgcatagg
    11101 taactggatg agtaaaccct gcattagaag tattcccaga aaactcctca aaatttctga
    11161 acaaaacgtc attatcttca gcaatcttat cttccatcac agtccagttg ctccaagctc
    11221 cattagtgct cgtacgataa cttgactgaa tcggtgcata actgaaggtc ggtgtcccat
    11281 ctcccacaac gattaggatt ttttctggat ttctattttc tccgccattg tctttataca
    11341 atgtttcata gccattttta agccctagtg taagcggagt tcctccgatt ggtgtacgat
    11401 ttaatgctgt attctcactt cccgtcaaac gattgatatc atttgtcaat ggaaagatgt
    11461 ccgaactgat ttgctcatgg ttattgatag ctgtactatt atggtaaaaa ttcaccatac
    11521 cgattcgaat atccagactc gggtcagaga gcagatcttt actcatttcg tttactgcct
    11581 caatcaacgc agcattttta gtttgactat tggtcccagc ggttaattca ctcatactcg
    11641 ctgacttatc tagaacaaaa acgatatcga ttggactgat ttcctttttc tcgcccccga
    11701 tgacatctag atagacattg taaagggttg ttgcatcttc aggatgtaga tcagatgctg
    11761 tgctgcttgg cagaacaggt tctacccatt ttttgatgta cgccccgtta taatccaagt
    11821 aactgttttc aaaattccgt tcatttcccc aaagctgaac ggtttgagct ggaacgtttc
    11881 tatcttcttc gcctgcagtt gatccattat tagtagcata gtcgtaattt cgccagttcg
    11941 ctcccccgtt tgtataatca ctatcatcga tcaagttctc tccgtcatat ggcgagcctt
    12001 tgtctccttg atagcgttca ttgctttgtg tccaatttcc taacactttt gtatagtcat
    12061 tcgttgtaaa cttcgggtag cgatgctgtt cgttttcaac ttctccgcta ggatagatat
    12121 attcataggg gtcttgcgta gctgttggcc caagacgctc caaaataccg ttgaataact
    12181 ggcgttgaat gcgctggagt gcttgaccgc tgggtacgat tggttcgtcc agcttttcca
    12241 tctcattttc ttcgctacgt tgaatttcag cggaatcttc tgtatcagtt tcattttctt
    12301 gtaattcttg gttatctatt ctttcatcaa ccaccacttg aagaggtagt tacccttcgt
    12361 tcacatcggg gatcttttct gtttcaaaaa tcaatgtgcc ggtttctgct tcttttgaat
    12421 aaacttttcc gacataccat tcttgaccat cgatcgtctc gacttttagg tcttctccat
    12481 cttcatcata ggaggataga cctgttccac gaacgttttt tacggtacca atcccggcgg
    12541 ctgccgcatc gattgctaaa cgtattctac cttcattggc actttccttt ttttcaaaag
    12601 ccagatgcca ttcaagggtt tcttcttttt caatatactt cccttcaatt tttgcatagt
    12661 cctcatctaa gaatacctca tcctcctttg tttctgttga ctcagcagct acatccaggg
    12721 tcgtcatatg atagactgac tggaaatatg gtccaaatag caaactgaca gttactaact
    12781 gcaaaagctt caattttaac cctttcacat ccttatactc ctttcaaaaa tatttgtctt
    12841 ctttcacaac aatcaacaaa acaatgccca tctaattata atttaattta attttaattt
    12901 taaatcaaat gatatccttt ttagttaaaa aacaccaatt atacaacatt tattattaaa
    12961 attaaattat aaaattttat aacgttctca tttaacaaaa cttaagttat taagacaaat
    13021 gaataaacaa ctacgctttc ctcgaaacaa aaaaataaag cacaaatgcg catttacgca
    13081 ctcatgcttt attcgtacaa ttaattcaga taacggcagc gcataggatg ccaataacac
    13141 ttcctaaaat agctcctcct aaaatgtcag taggataatg taaaagtaaa aaaatcctcg
    13201 aataagcgat caaacaagca aataaaaagg ctaatattcc tgcaactgga cttaattcga
    13261 acaacgcagc tactgataaa aaggctagcg ctgtatgtcc tgaaggaaaa gaagaagtct
    13321 tcggtttact tgtgtaaagg acaagccgac gatttttttc ataaggtctt ttccgtcttg
    13381 tcgttttttt gataaaaagc aaaccaacaa aaaaatagat caaactgccg ataagaatgg
    13441 ctagaccata ttctctagta tcaggtatca agactaaaac agcagcaagg agcaaccaaa
    13501 taaaaccacc attagagata ttactgataa atttcaaaaa attgattttt cgactatccg
    13561 ttttccgttg cttggcgaac ttgttgataa tactatcatc gattttagca atcaaacgca
    13621 acatgttatt cctccttttt aagaaccttt tcttgcgtac gatggataaa aagcaggact
    13681 tgaaaataaa acaagaaact gaaacctaat aaagcaaaat agattggtcc tgcaaaccag
    13741 aatgcgacaa gaaggatcaa cgtgcctgcc aaatagaaaa gcgcgctttt gaaatcatta
    13801 aaaaacaaga aaaatgcagt aatccaagaa gcttcttttt gttccagttg aagcaagatt
    13861 ttgtaactaa agagaagcgc gcttaataga agcaaaaaca acgtagtata catcaatgta
    13921 tagccaattc ccccacccca atagcggaaa atctgataat cgatcaatag aagaagaaaa
    13981 taaacacttg ctaaactatc ttgctttcca taaagagaga agttctcacg aaactttttc
    14041 cagaatagtg aaccgagctt tttctcttca ttttttttgt aatgataaat catatacaga
    14101 actgttcgac ttgcagttcc taatgtgaaa acaggcaaga cgcacaggaa ccatagtagc
    14161 tgtaaatgga taagacgtgg aatccattca atgatttcta agaaataatc attggctttt
    14221 ttaccaatcg atcgattcat catccttttg aacctcctgt caaatccatc ccttcaatga
    14281 aataacgttg tgcaaaaaag aacagcaaaa tcgtcgggat caagacaagt gttgctcctg
    14341 ccatcagata attccattgt gtcgttgctt ggttctgaaa aacttgcaag ccaatctgta
    14401 acgtgtattt ttcttgatct tggatatata acagaggacc taagaaatca ttccaagctg
    14461 cgttgaagga attgatcgca atggtaatca aggcaggttt cgtcaatgga agcatcaaat
    14521 gactccaaat atacaaatga ttcgctccgt cgatttcagc agcttcgatc aattcattat
    14581 tgatcgataa gtaaaattga cgcatcagaa atatattgaa cgcattgccg aagaacgatg
    14641 gaacgatcag gggcaaataa gtacctaccc agccgatctt ggagaataat acatattgag
    14701 ggatcatcgt tacaaacccc gggatcatca tcgtagaaag aaccaaagca aacatcagct
    14761 ttttcccagg aaaatcaagc ttcgcaaatc cataagcaat aaaagaattt gaaaggacat
    14821 taccgatcac tactagcaca gtgatgaata atgtatttct tgcatagcgc caaaaaggaa
    14881 aggcggcaat cgtatccaga tagttttccc atacgatttt ttctgggaaa aaggtcggag
    14941 gataacgaac gatttcttcc attggtttca atgaagtaaa caccatccaa agcaatggaa
    15001 tgatcaaaat gattcctatt actgtcatga ggacataact tagtattttt ttgtttgccg
    15061 attcacactg gtctttttga aataatccat catttgccgt cacctccaga ataatagacc
    15121 cacttaggtg caagcttcaa attgacaaac gtcagaacca acacgatcag aaacaggatc
    15181 attgacatag ccatcgcata acccatatca aaagctacga acgccttgtt ccacatatgc
    15241 agattataaa aaagcaaaga atttgcaggt ccgcctgttc catttttcgt catgacataa
    15301 gcttcttgaa aaatttggaa agaaccgatc gtagaggtga tcacatcgta aaaaataata
    15361 ggagtgatta atggcaaagt aatacttttg aactttcccc aaacgcctgc tccatcgatt
    15421 tctgctgatt catataaaga tctaggaaca ttttgtaatg tagctaagta aagtagcatt
    15481 gctcctccag aactccatat tttcatcaaa atcaacgcag gtttcgtcca ttgtggatca
    15541 aacagccaag ctggtccagg gatatggaac cagcccagaa atgtgttgat caatcctgca
    15601 gatggagaaa gcagctgcat ccataagatg tagactgcta ctcctgataa gactgccggt
    15661 aaatagaaag ctgtacggaa tacactgatt cctttgattt tttgattgag tactgtggct
    15721 aaaaacacac tgaaaatcgt cgtcagcggt acattgaaga tgacgaaata aacggtgttg
    15781 tataacgatt tccaaaataa atcatcgtga acgaacattc ttttgaaatt tgctagacca
    15841 atgaaattca tcttggatgt caaattataa tcggtgaaac taccaataac tgaaaaaagc
    15901 ataggcccta gcataaatac caagaacccg ataataaaag gcgagataaa taaataggcc
    15961 catcttgctt cttttttctt tcggatagat aaacgcttca aattttcttg cctcctctgt
    16021 acctagacag tcaaaaatgg attattgatt ttgcttgact aagttttcta ctgctttttg
    16081 tgcatctgcc aatccttctt ttggtgattt tgttcctaaa agaatctggt caatctgttc
    16141 attcactaat gtcgtgtaat ccggagcatc taaaggaact ggtgtgatca ccgtattttt
    16201 aaagttttcg tccgccattt gatagatcat ttttccttta tccgttaggt tgtcattatt
    16261 cactaaattc tcattggctg tcttgctcgc catgatatca aaactttttt cgccgaattt
    16321 ttcttgtact tctggagaag aaagatattt gatgaattca taagaggcct caggatcttt
    16381 tgctccgtat ggtacttcca agacgaatcc gccaccccac gaccaatggc ctgaaccttc
    16441 ttctttttct gggagctgca tcacgccaaa atcaaaatca tccggtgcat tttccatcaa
    16501 actagagtaa taattgatat tttgggcacg catggctact aatccggaaa taaatggatc
    16561 agatacacca gacccaaatt cggcttctaa tttattgatc gtatttttcc cataataatc
    16621 ttgccagtct tggatccact ccaaggcttc cactttattt ttagtatcaa tcttgacttt
    16681 ttcatctttg tcaaaccaac tagtcccgtt atctgcgttt agcgcccata catcagcccc
    16741 taaattccat aatggataaa aaccgatgcg tgtaaattct tttccttctt ttttatctag
    16801 tttacgtgct gtttcttcta attcatccca agttgtcggt agatcttgtt cactgattcc
    16861 agcttcttta aatatctttt tattataaaa aatgacttgt gtatctgtat tgaatgggat
    16921 cgcataagga tcaccttcat attctactgt atcccaaagc tgcggataaa aatccttaga
    16981 agtgtctttt tctacatatt tggagagatt agttgcttgc tgtgcttctg ctctttgggc
    17041 aacggaattt atgtcctgta caataacatc gggtgcattt ccagctgtta ttgcagataa
    17101 agatttagtc cagatgtcac cccatggctg atacttataa tttacatgga tctcgttttg
    17161 tgattgattg aaatcctcaa tgatttcttc gatagcttct tttcgtgcac cggatcccca
    17221 aaaggaccag aaatcaatat tttttcttcc atcacttgtt ttttcagaag aatttgctgc
    17281 acttgaactg gcatcgcttg cattacttga tccgcatcct cctaataaaa acaatgctga
    17341 acttgccagc aaccctgtaa acaataattt tttcatattt tacctccaaa ttttaatgta
    17401 accgatgccg gttttaatat aacgatatca ttttgttctg aaaataacaa tttataaggt
    17461 atgattctaa aaaaattatt aatgatacca atactttata taattcgcga gcgctttctt
    17521 tgaatttttt aatctttcgt agcagaatga gaatgtatta aaaatagatt aggaggaatt
    17581 ttgatgttta gacaccagaa agaattgcag tttgaagcaa aaccagatcg tccgaatcca
    17641 ttaatcgcga aatatctgca ggagctgatc ggcggccaat atggcgagat gagtgtagct
    17701 atgcagtacc tgttccaagg ctggagctgc cgaggagaag aaaaatataa agatatgctg
    17761 atggacattg caacagaaga aatcggccac gtggaaatgt tggcgacaat ggttgctcgt
    17821 ttattagagg gtgcgcctgt tgaagatcaa gaagcagcga tgcaaaaaga tccaacaatc
    17881 ggtgccatta tctctggaat gaatccacag cacgcgatcg tagctggctt aggacctcgt
    17941 cctgctgaca gcgtgggcaa tccatggagc ggtgcctata tcattgcgag tggcaacttg
    18001 ttagcagact ttagagctaa tttaaatgcc gaatcacaag gaagacttca agtagcacgt
    18061 atttacgaaa tgatcgatga tcgtggcgta aaagatatgc tgtctgttct ccttgcaaga
    18121 gatagctatc atcaaaatct atgggaatgt gcagtgaaag aattagaaga aaaagaaggt
    18181 tcaatcttag tcccaagtac tttcccgcgt gacaaagaac gaaaagacat tgcttatgac
    18241 ttctataact tctcagaggg agaccaaagt agtgaaggtt cttgggctag tggcaaagca
    18301 ctggatggcg aaggcgaata tcaatggttg aaagaaccta agatcgaagg cgctgcacca
    18361 aaattagaac cagtaacacc aaaaatgtat gggacacctc cattcaagaa ataaatatct
    18421 acccgtagaa acacccgttt tttgttccac acgctttgtg agcaaaactg actaaagtca
    18481 ttaatcataa ccacccttca aaagggtggt tttctctaga gctataagct caaatcaccg
    18541 gccagcgcct aaagacgctg gctttcatat tttatgttca agccatattg cctttgccag
    18601 ttttgcccac ctcttaaaag gtttatgtac tactccttag cccttaaaag gatcgtctta
    18661 ctctcgtaca ctcagcttat ctaacgcact atcatgtttt tcttgttctt gaatgtactt
    18721 tttgatggtc gcttcatttt gtcctacttt tgtcacatag tatccttcca cccaaaagtg
    18781 tcgataccca aatttatatt ttagatttgc acaatctctt gatgatctcc tgaatattgg
    18841 ctcgatattg attataaatt atttttcttc tatactttgg tgtgaataca atgtggtatt
    18901 tacacatcta ctttgtacgg gctaaactat ttccctttat tgctccaccc gcatagcaag
    18961 tggttttgta tttcaatcgc tcggcgcgct caattaggtc taaagacaat aaatctaaaa
    19021 acccgctgct tcactgacag aaacaacggg ttttcagaaa aaaatgaaat gaaaaagatc
    19081 tcttacggca atctatttta tttttttgat tttttataaa tggaaaatgc ttcaagaagt
    19141 ccaagaaata aagccgtata aaaacaagaa cctatgaaat ctttatttat tagatattca
    19201 acagaaatcc cgataacact agccaacaat atatttatca gaagtgtaaa ataaggatgc
    19261 tttttataga atagaactag tgttgttttc atagactctc ctttcttcat atttccaatt
    19321 aatcacaaat tcacggcatt actagaaaaa ttaaaggggc gcagaagtgg aaaaatactc
    19381 ttcgactttc cctacaaacc aggccatctt gtgtttaata actataccaa atctaaggta
    19441 cctctaaatc taaattccat ttgtggcggt gacctccaat ttttaactct tctatctccc
    19501 atccagtata cataaccttg atacttcgac cacttatata tcgaacacac aaatacctat
    19561 ccattgcagg agtactacct aacttagttg ctacttgcat ctaaataaca gctgaaaaga
    19621 taggacttac tcttagtaaa gtatctgaac tattaatttt taaactatca tttttatcct
    19681 caactacatg cacatctgtt gcaacttcac tttctgtctc atccagataa atgattaagg
    19741 aatcgggaat tgcaatatct tcagctgacg ctggaatagt tgagctaaat tagtcatcat
    19801 atctttatta taaaataacc aacagcaaaa aatacaatga aaaacaaact tggaactaat
    19861 gtatcattaa taaatgttga aaaattcagc aactcattac tgtatgattt tagcagccca
    19921 accaacatat accctataaa agctaataag atggttagga atcctttaga atttttactc
    19981 attcatagac acttccttaa ataactttta ttatagaagg tattgtaaat aataatattg
    20041 acaaaaagtg actcaaaata aaaaaaacct tacattatac tggaatgaac tgtattgata
    20101 gtgtacctaa aagctctact gacataagat cgtgtaatcg gtaaagtaat tttattaatt
    20161 atacgggatg taatcaatta gagaataaac taatcataaa aggtactttt acattcataa
    20221 ttgtcgcttt gagattctac tttggttcac tatctgaccc aattgcacat tttcgatcat
    20281 tacaagtatt ggtgtcgtta gaacaactaa ataaaaacta gcggtagatt tttcagaatc
    20341 tatgctaatt tctgtatgga tgttccagtg atggcaaatt tctcttttca ttgtttttct
    20401 tgtcgcatga tatgcctttt tgtgttattc agttaaacta tagaaattct ttcctagaca
    20461 gatcttgatc ccaaatttta cgtttttgag catcgatata gtttaaaatg acattactca
    20521 tatctaaaga gtttagattt tttaggacta aaactgtcag ttgttctttt tcttctttct
    20581 gttaaagtat tgtcgcgact tttcgtatac gttaagatat gtcgtgaaaa aacaagtttt
    20641 gtgacacaaa aaaacgaatg acttgaggaa atcacctcaa tcattcgttt aaaatgctat
    20701 atattcttta gtttaagata cggaaattaa tgccatcgac cacactagta gaatgaacga
    20761 catttacttg gccttttttt aatttcgtta tgtttagcat ataaaaatat tatagagttt
    20821 acttacaatg cttaaaacat atactgtata atctgatcaa ttttagccat gtcttgcaat
    20881 ggtaagtttt ctattttttt gagttttcgt tccttaaaat caagagattt taattgtgta
    20941 ataattactt gtccttttat atctagctct tcaggtaatg agtaacgtgt cggtaaattc
    21001 tttatagtgg aagtaattgg acatatgacc gaaaacattg ttttacggtt aaattcataa
    21061 cgagagacga ctagtcctgg tcgtcttttt tgaatttctt ttcctacgga gggatcaaaa
    21121 tcaatccata caatatcacc ttttttagga atataaattc tttcttctga cactataaaa
    21181 tttctctccc ttcaggagtt aaatcttccc attcgtcttt ctcataatac tcggcctctt
    21241 ccccaccact aaatggatct tcaatttttg gaactaacgt aattgttcca tcatcagaat
    21301 acacaacgat atattcttgg ttttctgaag gtttctttcc gttatcagaa ggtaaagtta
    21361 ccactacaga acttccttgt aatctagatt ttgtaacaag cattatcatc actcccttaa
    21421 taaaagtata ctatataaca ctaagtaacg caaaattccc ctttgtaaac tatctagaaa
    21481 attgttttgt ttaaagtata tcttattgga tataaataga aataaattgt agccagttca
    21541 ttttttcttt aactaaaaaa ataatccaag aaacattgaa cctatggatc caaaagctac
    21601 ccaaatacta tagctaaccc cccgctacct tcaattttgc agataaaacg ctttgggaga
    21661 aaatttcaaa gacaacaggc catcctatca tgcccgatta taaacaaaaa gctggctatt
    21721 ccttttgctc tgacaaattt caattgggaa agttcacgaa actagcaggt gaagaagttt
    21781 caactgttcg gatcaaagaa tgcccgcttc aaatagaaac attggttcaa cgaatttcaa
    21841 ctagacaaga ctttgctatt gtagaacgtg agatcgtagc tatttttgta gaagaaggga
    21901 ttttacatga tccaactcat atcaatgtgg ataaatggca acctcttatc tataaattca
    21961 gagaatatac aactgccaac cagtcgttaa ctttgaattt tagattccaa gaattcaaag
    22021 agtcataatg ataaacatcg ttaggtataa ataaaaaaga ctcgcagcac gagtaaaaaa
    22081 caaatagaag ctatacatta gaaaggtcga ctgtgaccct atagatgcag tatatgcagc
    22141 ttatgagcat ttcttctgtt ctctttgttt ttgggatctg tgctgaagtc tttttcgttt
    22201 tttgccgctc tttctttgaa aaatcgatta tgttatacct tctccattac taattcaggc
    22261 atctgctgag taatgatttt tcgcgcaaaa gaaatatctg ttacccaagg ttcgatttgt
    22321 tcttcagtca agacaagcgg cattcggtca tggattggtg cgactgtgcg gttaggttca
    22381 gtagtcatta tgattgattc tgccattttt tcatcctgat ggctacgata aaaaccgcca
    22441 atatacacta ttttttcctt atttgaaaac ataaatttgt ctttttcttc attccactca
    22501 ttgggaaaac acaacgattt tgcaaaaatg gttgatgaaa ggtctttttt tcttctactg
    22561 tctctgcacg ggcattaatc atcaattgtc cttttttaaa tccggtaaat ccccacttca
    22621 tgatttccgg agtgaggatg ttttcttcct cttttcttaa cgtcacaaca agatttgacg
    22681 gaaaaacttc tccagctgct aattctgctg cctgcttcgg ttgtttcgtc tgggcttctc
    22741 gataataatt ctgaaggagc tctgactcta gatcaaaaaa ataacgacca cacatcctaa
    22801 tccctcccaa tctttttttc tatcatacac tgcaacaaga agttcgcacc aacaaaacgc
    22861 tttcaatttt tgtagaacta aaaaagttgt gacaagtgac aaactctcca gtgaatggta
    22921 ttctcctgcc aactcctcgg tcacaagtca caatattcaa caaacatgag aagctgcttt
    22981 ttgaaattgt tccttgtgac tcggagtgaa acggcaggct tacgacctct gggtaaacgg
    23041 tgttcaaaag ccagtttctc ggtattaagc caaatttatg caaaaatgta gaaagctatt
    23101 ttcacaaaat ttttctta
//
LOCUS       DACGLE010000041        22184 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0534
            SAMEA3304449-rid9560453.denovo.042, whole genome shotgun sequence.
ACCESSION   DACGLE010000041 DACGLE010000000
VERSION     DACGLE010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304449
            Sequence Read Archive: ERR985064
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22184)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22184)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702225.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702225.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 20:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,045
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 27 of 67
            Pseudo Genes (incomplete)         :: 25 of 67
            Pseudo Genes (internal stop)      :: 19 of 67
            Pseudo Genes (multiple problems)  :: 4 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22184
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304449-rid9560453.denovo.042"
                     /strain="VREN0534"
                     /db_xref="taxon:1352"
     gene            complement(22..2700)
                     /locus_tag="IXT20_001694"
     CDS             complement(22..2700)
                     /locus_tag="IXT20_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4860140.1"
                     /translation="MKIIEKKIANKKINWLVVNFLVLIAMIIFTSNIAHASIFNWFDD
                     TKSQTEFLNNSLYRPYLKKYNGDFLSQFGIWLGWAVVKGMFTVTDSIQNMIPDVLDLF
                     NFIESTGLNNVYQSAVNTIVIGLMILSLMFVGYKMITGKGTVDLKSVGMNVVMSIALI
                     LLMPTMISSGIQFAKTFYKDSTTITNSDDGVAWSLIKQGVTDLAYINKTDQYSSIDKT
                     EDRNNLTRKNFQQTDLTQVLTDKVIDKLEKENPIADNLRYELVENSNNEFVATKFSDN
                     FLSTFSDSLKSGYYRYQANLWGISIGLTALAIAYVFSAFVIITAILELAFKRVLGVLV
                     FATDIETGQRSKVVLSDILQCYLTVGFQGFGLSMFAMFINFLNSGQGISTNIFIKTIA
                     YICAVFVLIKGSGTVMRYFGVDIGLKEGYGQLASAFGMGAMLFRKGSNGFNRAKGSGE
                     GEDRKPEKNFGETLSKKAGKTGRALGYAHERGLSGLASDGATMASERATKPFKSMRDI
                     ANDTKNKFKEGLDDGTVSAINKNSKPMLAKNKEDETGKYADSVPTRLSDRKDGAKVEN
                     ADRIMSSSERMREAMKNNAESNNNPASAIQQKVQQDIEERKNAMHGQAKSAEELINQK
                     RHEAKYTPEAMNREEIMLRKRVEGRTGANIDGKEALTKERIQEAKNSNTGLEKLVKEN
                     PQNSTSSHGGRSVDVRENIQGSLNGHVGRNVDVRENLQSSSNAQGTKTIDVRENLQSS
                     STSQPKTVDVRENVQNSTSSQGFKSVDVRENVQGSSASQPKTVDVRENVQSSTSSQGF
                     KTVDVRENIQKDSGEMKEKTQKINIVEDRKSAKKFDSNHETVIIDSEIRENDKGSKSR
                     RRFTYEDNELFRDTLNDPNPLFDNLLRNKRGGLSCN"
     gene            complement(2687..2905)
                     /locus_tag="IXT20_001695"
     CDS             complement(2687..2905)
                     /locus_tag="IXT20_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4860141.1"
                     /translation="MIKNRLIDQVWCLDQNTDSLELIETICFNTIVLDLRAENDNCVT
                     HVIINQKMAQKLSESLRKWAKGGNDENN"
     gene            complement(2981..4033)
                     /locus_tag="IXT20_001696"
     CDS             complement(2981..4033)
                     /locus_tag="IXT20_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HAQ4860142.1"
                     /translation="MNEKEVESLVEKQGYSNSDIYAELYYQYPIFLNVNEKYKKMKLS
                     SKLVYMLLKDRNRLSLKNNLYDENQKIFLIFEIKNLVEISGMSKNTILSALDELQKVK
                     LLVKRKNGFNKSKMQMFPNFYYLLKPVLCKDDVYKINKITHQENRQKNLIVQNLNYGT
                     NQYSVDNSKFELCDTSMKTRDKNNFADSSKNELELYKSYKDSKDIKESHNDDLLESLS
                     TKNNKLDEELIETFIEENLLKQIWGESIVNCLKLASNSDYRQFDNWYKKFKYAIRSAE
                     KEQKIQLKIIYEICNNKYFVDHVREQLSMTLRDLIRRAKTDHRIKQKDDYIFASLKNK
                     ALELIELQISEGIDKQ"
     gene            complement(4186..4512)
                     /locus_tag="IXT20_001697"
     CDS             complement(4186..4512)
                     /locus_tag="IXT20_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4860143.1"
                     /translation="MASWLIEEIENERRKIMDAGITVMLDKQQTNQLKNYVFEMTKEA
                     IDQARIDTGLERPFLKGKEMAKYLNVSYTTFLKFKRMGLPVILLEKMELFSKEECKKW
                     ILSHQI"
     gene            complement(4524..4724)
                     /locus_tag="IXT20_001698"
     CDS             complement(4524..4724)
                     /locus_tag="IXT20_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ4860144.1"
                     /translation="MITLNDQFIRSLRRHRADLILTKNDAAKLIGINRKTYVKIENGS
                     KESIRASTYQKLVNWLLNDLKI"
     gene            4799..5449
                     /locus_tag="IXT20_001699"
     CDS             4799..5449
                     /locus_tag="IXT20_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ4860145.1"
                     /translation="MSIKILLNLWFSCYNSLEFTIGGVTLNRIKFYIDSSKKTIKQIS
                     TETGINYTTLTNYYRGERNPRNTETWNILADYFHVPVSYLMGLDETNSIEQPNLDRIK
                     TARLEKKDKIVKNIASLDSDYFTELSNLYFSMTSLLEVKLQEDDSDFLFLKSILEDSF
                     EYTILFAKQKKFINKALNLNIDERKELESELFGNLTQVQVNNLKREITARLISKLQ"
     gene            5504..6670
                     /locus_tag="IXT20_001700"
     CDS             5504..6670
                     /locus_tag="IXT20_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAQ4860146.1"
                     /translation="MSIQKYTTKSGARYSVRLYVRKDENGKQEYYVKKGFKTEKEAKL
                     HEARKKVEIEDTGYHKPSNDLFITVYEEWLPSYRNRVQETTYQRTKDLFRLHILPKFG
                     NKSISKITPVHCQNAVNEWALKFKNIKQLKSYSSQIFEYAIFAELLHRNPMANTNLPK
                     REKKENENYFSLEELKAFWDILNKEEPLKHILIFQLLITTGIRKGELAALHWSDIYFE
                     EQLMYVRKSYATIRNEEKNARRKTVRIQKNTKNISSERILPIDAQTIEMLKIWKKEQA
                     RELLQFGINTNTKNQLIFTYVNADGDINQPLHADYSNNILKRLEKKYKFKHVTIHGFR
                     HTHATLLLEGGASIKETQDRLGHKNAETTLNTYSHVTEKAQRNALEKFIAYTGL"
     gene            7075..10302
                     /gene="fss3"
                     /locus_tag="IXT20_001701"
     CDS             7075..10302
                     /gene="fss3"
                     /locus_tag="IXT20_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding MSCRAMM adhesin Fss3"
                     /protein_id="HAQ4860147.1"
                     /translation="MYKKQKKKLCVLTILSLAVQAIVGAIAPTIAFADEITHPQTVTV
                     ELDLAHKYAVEGTFSDGRPMSEVTVPHYAVYNGVKQDIFCIEPGVPIDNEFTPGYEKN
                     PLPDMPEKAKLVSVLWKKAGTDVDTHIVAQKMIWQEVNGYTLHSIKRLNGSAVNIAAI
                     EAKINQAIADYQKKPTFHNSTAKTVLGQSTTVTDTNNLNLSEFDEVVENTANIDYRVN
                     GNQLIITPNANSKESGVLTLKKSAGTGTPVAYKKAGQQTLMAGAIDKPNTYTIKIDVE
                     TEGSLKIKKVDKESGDVVPGTVFHLDFGKTLPAKDVTTDKEGIATLDEIPHGTKVTIT
                     EKSVPAPYTIDTTPMTTTIKAGETIYVTSKNAQEKGQIILDKSGVETGSDLWNDNYSL
                     AGNTFAIRKDSPTGEIVQEMTTDENGHAETPKEIANALELGTYYVTETKASHGFVNTF
                     KPLKVELKYANQTVALVTSNVKGQNQEVTGETTLTKEDKDTGDKAQGKAVFEGTEYTL
                     FTAKDGKAVKWSEAFKPEMVKGTKASDETVTLALDEKNQAAVKHLAINEYYWQETKAP
                     EGYTLDETKYPVSIKKVDDNEKNAVITRDVTAKEQIIRFGFDFFKFAGSAAGTAETGF
                     NDLTFKVSPLEGTNEITGAEDEATTAYNEQLGFDGYGKFENLPYGDYLLEEVEAPEGF
                     QKITPLEIRSTFKENKEDFVKSEYVFTITEQDQKQPIKTVTVPYEKLTNKAFSVSLNR
                     LMLYDLPEEEDSLTSLATWKDGNKELTSLDSTELVDKLSYNLHEIKEDWYVVAQAIDV
                     DATKAAQEKDEKAKPVVIAETSATLANKEKTGTWKIQHKLTAEQVLNKTIVLFNYVYE
                     NKEAFEAGDKPVAKDVSLNNQAQTVNCTVERHVSIQTKAHLENGSQTFTHGDVVDMFD
                     DVSITHDVLDGSKEAFETILYALLPDGTNKEIWKSGKIEYEVNDKEFTKTVLAKKVDT
                     GKYPEGTKFTFAEINYDKDGTINGKHNEDLKEKSQTLTPKEVPTILSTPKQPETPTVP
                     SGSQESSLTVKTFPQTGEKNSNVLLFIGFTLIFATAGYYFWNRRN"
     gene            10415..10729
                     /locus_tag="IXT20_001702"
     CDS             10415..10729
                     /locus_tag="IXT20_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAQ4860148.1"
                     /translation="MELKFVVPDMAETFGKLSYAGEGEVLTEGYGRNTTVIGRSYHLY
                     SSKQRADDIEVVVAAEAGEKDFDQDQPLKAVNPHLVAKGYEIENRGFTDYVLYVDDLV
                     KA"
     gene            10742..11116
                     /locus_tag="IXT20_001703"
     CDS             10742..11116
                     /locus_tag="IXT20_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAQ4860149.1"
                     /translation="MRLTEGIVVDSGLTFGKLRFSALRREVRKQNEDGTIGNEVKERT
                     YNLKSSAQGRMIQVSIPANVPLREFAYDAEVELVNPIVDTVANYVFREGTTVNWFIKA
                     DDLVLKRQPNQGNPTNQNERKK"
     gene            11126..11470
                     /locus_tag="IXT20_001704"
     CDS             11126..11470
                     /locus_tag="IXT20_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein J"
                     /protein_id="HAQ4860150.1"
                     /translation="MEINVEHILDCLDQYGKGELTEEQLTNALTYDEKMFLIMHQGLL
                     EVGNDAKEDFDIPDWLGEEESFFMVVEVNESLCRQAESVLEEIGVEMPDAIEGFLKQL
                     VETKQLPVVVND"
     gene            11539..12555
                     /locus_tag="IXT20_001705"
     CDS             11539..12555
                     /locus_tag="IXT20_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HAQ4860151.1"
                     /translation="MYKGHRIRAGDQHLIYHFVLGWLSALFIGWMGVFYFPELRQFGL
                     SKVSFSTIEIAWSMKDLVCLLGSLALSGSMMLLYIHFFPDHWRSLWNRQKLARMILEN
                     HWYEVKQTQSDGFFKDLNRSRTKETISYFPKVYYRMKDGLLSIRVQISLGKYQDQLLK
                     LEKKLESGLYCELVEKELKDSYVEYTLLYDMIANRIGIEEVVAENGTLRLMKNQVWAY
                     DSLPHMLIAGGTGGGKTYFLLTIIQALLKSDAELFILDPKNADLADLGTVMPHVYSQK
                     EEISKCVEDFYERMMARSRSMKEMPNYKTGENYAYLGLPPNFLIFDEYVAYMGANRFP
                     TSIE"
     gene            13149..15023
                     /gene="ltrA"
                     /locus_tag="IXT20_001706"
     CDS             13149..15023
                     /gene="ltrA"
                     /locus_tag="IXT20_001706"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HAQ4860152.1"
                     /translation="MNVETDEVQGSGKNVGKSVMEVPRLNTKIRYWEYYDLQETFDQL
                     FTQSRNGKKFYQLYELIISENNILLAYRTIKANKGSSTPGTDSFTIDNYKEMNQAEFI
                     HLILSHLENYKPKSIKRVMIPKPNGEKRPLGIPCMIDRMIQQMFKQILEPICEAKFYE
                     HSYGFRPLRSAKHALGRIMYLINISKMHYAVDIDIKGFFDNVNHRLLIKQLWNIGICD
                     KQVLAILSKSLKSPIQGEGIPSKGTIQGGIISPLLSNVVLNDLDHWVSKQWHTFETKY
                     PYTKGYNKFRALRDTNLKQGYIVRYADDFKIMTNDYPTALKWFHAVKLYLKDRLKLDI
                     SNEKSKIVNLRKCKSEFLGFAICVKQKGKKWVCNSRISNKKKDQIKEEIKQRIKDIQK
                     SPTAQNALLFNALVLGVHNYFRYATHINLDLNRMNYVLSFTTYNRLSNCSTRCYPNKA
                     SPTYKKFYSTTMKTYKVAGVYLYPLCDVSTKTIYGFNTKDTPFTPFGRQRLEHKSLQS
                     LVFQELRKLMESKIPNRTVEYLDSRISRYSMKSGKCEITKQFLPAKAVHCHHYLPKSL
                     GGDDKFDNLRIIHKDIHLLIHTTNKMIIDHYVHELKLVPEQIAKINLYRKMCNLQNIQ
                     "
     gene            15095..15478
                     /locus_tag="IXT20_001707"
                     /pseudo
     CDS             15095..15478
                     /locus_tag="IXT20_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010759325.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            15556..16230
                     /locus_tag="IXT20_001708"
                     /pseudo
     CDS             15556..16230
                     /locus_tag="IXT20_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297358.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
     gene            16463..16690
                     /locus_tag="IXT20_001709"
                     /pseudo
     CDS             16463..16690
                     /locus_tag="IXT20_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364177.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
     gene            17168..17317
                     /locus_tag="IXT20_001710"
     CDS             17168..17317
                     /locus_tag="IXT20_001710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4860153.1"
                     /translation="MNRLNHRNAVRSGRGVITHEEAVIGKTILYNLFLSEGKRSHSTD
                     EVMET"
     gene            17451..19301
                     /gene="ltrA"
                     /locus_tag="IXT20_001711"
     CDS             17451..19301
                     /gene="ltrA"
                     /locus_tag="IXT20_001711"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002588016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HAQ4860154.1"
                     /translation="MKKQKLRNAEYYDFQTIQDQLYSQSKHGKIFKHLMDIISSDQNI
                     KLAYRNIKKNSGSHTPGVDGKNIENLAKWQDDALIKHVKRKFQWYEPQAIRRVQIPKS
                     SDSTKTRPLGIPTIMDRLIQQCILQVLEPICEAKFYDKSNGFRPNRSCEHALAQAEKN
                     MQLSKLHFVVDIDIKGFFDNVNHGKLIKQLWTLGIRDKKLICILSAMLKAEIAGVGFP
                     EKGSPQGSIISPLLSNVVLNELDWWISSQWETIPTKKEYKQCDGHSSGRLQALKKTNL
                     KECRLVRYADDFKIFCRTRSDAEKIFVATEKWLKERLGLNISPEKSRIVNLKKHYSEF
                     LGFRLKVVRKGKYPNGNPRFVIRSHISKKALKNIHLSAKKRISNLQNLSDDRECYKSV
                     QNYNIFVLGIHNYYSKATHASLDFAKIAFPIRKSLKGKLQGNFSKEKGICSSLIENRY
                     GKSRQLAYVYGFAFIPIGYVQHRAPLDKKREINQYTAEGRELIHKQLGSSINPDLLSY
                     LMENPIKGKSIEYNDNRLSLFCAQKGRCAITGDDLTIGNMHCHHKLSLKLGGTDEYKN
                     LAFVTVEIHRLLHATNLKIIIELVQKLNLSRTQLEKVNKLRKKIALPIIE"
     gene            19436..19642
                     /locus_tag="IXT20_001712"
                     /pseudo
     CDS             19436..19642
                     /locus_tag="IXT20_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364177.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
     gene            19643..20351
                     /locus_tag="IXT20_001713"
                     /pseudo
     CDS             19643..20351
                     /locus_tag="IXT20_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297356.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
     gene            20341..20631
                     /locus_tag="IXT20_001714"
     CDS             20341..20631
                     /locus_tag="IXT20_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002395166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4860155.1"
                     /translation="MIYDELVGEIYWVIGKIQSDPELEEQLRRLNFDIRKNGVKVAGD
                     PYLMNEETDARIEINQVIAEFERIADQAKEPDIRQYLLEMKAELEVNGITDE"
     gene            20889..22073
                     /locus_tag="IXT20_001715"
     CDS             20889..22073
                     /locus_tag="IXT20_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HAQ4860156.1"
                     /translation="MVQQNLDYRLLKDRRNQYGVSQNKLATACGLSRPYLNQIENGGV
                     TTSTKTMRKIFDQLESFNPDLPLTLLFDYVRIRFPTTDVQKIVQEILHLKFDYMLHED
                     YAFYSYQEQYIMGDIVVMLSHEEEKGVLLELKGRGCRQFETFLLAQKRSWYDFFDDCL
                     KAGGVMKRLDLAINDRVGLLDIPELTKKCQKEECISLFRTFKSYRSGELLKADEKDGM
                     GNTLYIGSLKSEVYFCLYEKDYEQYIKLGIPLDKSETKNRFEIRLKNDRAYHAIQDLL
                     KGRSIEGTTFSIINRYLRFTDKVEGKRRTNWPLNERWGRFIGRNRKEIQLTSDPKPYT
                     IERTLNWLGRQVAPTWKMAKELDRLNQTTYILDMVQNAQLSDRHKKILEQQSMAIENL
                     II"
     gene            22070..22168
                     /locus_tag="IXT20_001716"
     CDS             22070..22168
                     /locus_tag="IXT20_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HAQ4860157.1"
                     /translation="MKRGSSMLGVTVMCLIHTSAAADRVMERKVIK"
BASE COUNT         7410 a         3825 c         4293 g         6656 t
ORIGIN      
        1 catgcgtact aatctttaga attaattgca tgacaatccc ccccttttat ttcttaataa
       61 attatcgaaa agtggattag gatcattaag tgtatctctg aataactcat tgtcttcata
      121 agtgaatctt cgtcttgatt ttgatccctt atcattttca cggatttcac tatcaataat
      181 cactgtttca tgatttgaat caaatttttt cgctgacttt ctgtcttcaa cgatattgat
      241 tttttgtgtt ttttctttca tttctccact atctttttga atgttctccc gaacatctac
      301 tgttttgaat ccttgactac ttgtagaact ttgtacattt tctcggacat ctactgtttt
      361 tggttgactt gcactgctac cttgcacatt ctctcggaca tccactgatt taaacccttg
      421 actacttgta gaattttgta cattttctcg aacatctact gtttttggtt gacttgtact
      481 gctactttgc agattttctc gaacatcaat tgtcttagta ccttgagcat ttgaagaact
      541 ttgtaaattc tctcttacat caacattccg tccaacatga ccattcagtg aaccttgaat
      601 attttctcgg acatctactg atcggccacc atgactactt gtagaatttt gtggattttc
      661 tttgactaat ttttctaatc ctgtatttga atttttagct tcttgaattc tttctttggt
      721 caacgcttct tttccgtcaa tattagcgcc tgtccgccct tctacacgtt tccttaacat
      781 tatttcctcg cgattcattg cttcaggcgt gtatttcgct tcatgacgct tctgattaat
      841 taattcttca gcagacttag cttgaccgtg catagcattt ttacgttctt caatatcttg
      901 ttggactttt tgctgaattg cgcttgcagg attattattc gattcagcat tatttttcat
      961 tgcttcacgc atacgttcag aactagacat aattctatcg gcattttcaa ctttagcacc
     1021 atcttttcta tctgaaagac gtgttggcac ggaatcagca tattttccag tttcatcttc
     1081 tttattttta gctaacatag gtttgctatt tttattaata gcggacactg taccatcatc
     1141 taaaccttct ttaaacttat tcttggtatc attagctata tcacgcatac tcttaaatgg
     1201 ttttgttgct ctttcactag ccattgtagc tccatctgaa gctaaaccgg ataatccacg
     1261 ttcatgggca tatcccaaag ctcttccagt tttgccagct ttttttgata gagtttcgcc
     1321 gaaattcttt tctggttttc gatcttctcc ttctccgctt cctttagcac gattaaatcc
     1381 atttgatcct tttctaaaca gcattgcgcc cattccaaaa gcagaagcaa gttgcccata
     1441 gccttctttg agtccaatat caacaccaaa gtatctcatg actgttccac tgcctttgat
     1501 taaaacaaaa acggcacaga tataagcaat tgtttttata aaaatatttg ttgagatacc
     1561 ttgtcctgag ttcagaaaat tgataaacat agcaaacatt gataacccaa atccttgaaa
     1621 cccaacagtt aagtaacatt gtagaatatc tgatagaaca acttttgaac gctgtcctgt
     1681 ctcaatatct gttgcaaaaa caagaacacc taacacacgt ttaaatgcaa gttctagtat
     1741 tgctgtaatg atcacaaacg cgctgaacac ataggcgatt gctaacgctg tcaacccaat
     1801 tgaaatcccc catagatttg cttggtatcg gtaatagcct gatttcaagc tatcagaaaa
     1861 tgttgataag aagttgtcag aaaactttgt agcaacaaat tcattattag aattttcaac
     1921 taattcataa cgaagattgt ccgcaattgg attttctttt tccaatttat caatcacttt
     1981 atccgttaac acttgtgtga gatccgtctg ttgaaaattt tttcgtgtta aattatttcg
     2041 atcctccgtt ttatcaatgc ttgagtactg atctgtttta ttaatatatg ctaaatctgt
     2101 tactccttgt tttatcaatg accaagcgac tccgtcatct gaatttgtaa tcgtagtaga
     2161 gtccttataa aatgttttag caaattgaat accactagaa atcatggttg gcattaaaag
     2221 tattaatgct atagacatca ctacgttcat tccaaccgat tttaaatcca ctgttccttt
     2281 tccagtaatc attttgtaac caacaaacat caatgaaagg atcattaatc caatgacaat
     2341 tgtatttacg gctgattgat aaacattatt tagccctgta ctttcaatga aattgaacaa
     2401 atccagcaca tctggaatca tattttgaat cgaatctgtc acagtaaaca tgccttttac
     2461 cacagcccat ccgagccaaa tcccgaactg actaaggaaa tctccattgt acttttttag
     2521 gtaaggtcta taaagagaat tattcaaaaa ctctgtttga ctttttgtgt catcaaacca
     2581 attgaaaata ctcgcatgag caatattact tgtaaaaata atcatagcta tcagaacaag
     2641 aaaattaaca accaaccaat ttattttttt attggcaatc tttttttcaa ttattttcat
     2701 cgttacctcc cttagcccat tttctgaggc tctcagagag cttctgagcc attttttgat
     2761 taattatgac atgagttaca caattatcat tttcagctct taaatcgaga actatagtgt
     2821 tgaagcaaat agtctcgatt aattcgagac tatctgtatt ttgatctaga caccaaactt
     2881 gatctattaa acgattttta atcaaaataa cccctcctat attcaaagtt ctcaaatgaa
     2941 gtggagatac ccctccattt catttgactc aattctattc ttattgctta tctattcctt
     3001 cgctgatttg caattcaatt agttctaacg ctttattttt tagagaagca aagatataat
     3061 cgtctttttg tttgatacga tgatctgttt ttgctcttcg tatcaagtca cgtaaagtca
     3121 ttgatagctg ttctctaaca tgatctacaa aatacttgtt attacaaatt tcatagataa
     3181 ttttcagttg aattttttgt tctttttcag cagatctaat cgcatatttg aattttttgt
     3241 accagttgtc gaattgacga taatcactat tgctagccag ttttaagcaa ttcacaatac
     3301 tttctcccca tatttgttta agaagatttt cttcaataaa tgtttcgatc aattcttcat
     3361 ctaatttatt atttttagtt gaaagtgatt caagaagatc atcattatgt gattctttaa
     3421 tatctttact atctttataa gacttatata attctagttc attttttgaa ctatcagcaa
     3481 aattgttttt gtctcttgtt ttcattgatg tgtcgcatag ttcaaatttt gaattatcaa
     3541 cagaatattg atttgttcca tagttcaaat tttgaactat caagtttttt tgtctatttt
     3601 cttgatgggt tatcttatta attttataaa catcatcttt gcacagaaca ggctttaaca
     3661 aataataaaa atttgggaac atttgcattt tacttttatt gaagccattt tttcttttca
     3721 caagaagttt aactttttga agttcatcta gtgctgataa gattgtattt tttgacattc
     3781 ccgaaatttc tacgagattt ttgatttcaa atatcaaaaa aattttttga ttctcatcat
     3841 acaagttatt tttcaatgaa agtctatttc tatcttttag taacatataa actaacttag
     3901 aacttagttt cattttctta tatttttcat ttacgtttaa aaaaatagga tattgatagt
     3961 ataattctgc atatatatca gaattagaat agccttgctt ttcaactaag ctttcaactt
     4021 ctttttcatt cattgtcaca cctgcttctg attttatttg ctataataga ttcaaataaa
     4081 gttttctcag aactttatta ttgagtgttc ccccgatctg caaactgacg ggaacactct
     4141 ttttaatttt ttctacttca tcaaatttaa aaaacgaggt gttttttaaa tttgatgact
     4201 caatatccat tttttgcatt cttcttttga aaacaactcc attttttcta ataaaataac
     4261 tggcaatccc attcttttaa atttcaaaaa agttgtatat gatacattca aatatttagc
     4321 catctctttt ccttttagaa atggtctttc taatccggta tcaattcttg cttgatcaat
     4381 agcctctttt gtcatttcaa aaacataatt tttcaattga ttcgtttgtt gtttatctaa
     4441 cattactgta attcctgcat ccattatttt tctcctctca ttttctattt cttcaatcag
     4501 ccaactcgcc aagttgtatt tatttagatt ttcaaatcat taagtaacca atttaccaac
     4561 ttttgataag tagatgctct aatactttct ttgctcccat tttcgatttt gacatatgtt
     4621 ttccgattta tacctatcaa ttttgctgca tcgtttttag taagtataag atcggctcta
     4681 tgtcttctta agcttctaat gaattgatca ttaagagtta tcattcagcg cctcctttca
     4741 aattattcac tttgtggttt ttatcttaat aagcacattt taaaccacaa aacgagtaat
     4801 atcaattaaa atattactca atttgtggtt ttcatgctat aatagtttgg aatttactat
     4861 agggggcgtt actttgaata gaataaaatt ttatattgat tccagtaaaa agaccattaa
     4921 acaaattagt acagaaactg gtattaacta tacaactctt acgaactatt accgtggtga
     4981 gcgaaatcct agaaatactg aaacatggaa tatccttgct gattatttcc atgttcctgt
     5041 aagttatttg atgggattag acgaaacaaa ttccatagaa caacctaatc ttgatagaat
     5101 aaaaactgct cgtttagaaa aaaaggacaa aatagtcaag aatattgcca gtcttgactc
     5161 agattatttt actgaattat ctaatctata cttttccatg acttctttac ttgaagtcaa
     5221 acttcaagaa gatgactccg actttctttt tttaaaaagt attctagagg actcctttga
     5281 atacacgatt ctatttgcca aacaaaagaa gtttattaat aaagctttaa acttaaatat
     5341 cgatgaaaga aaagagttag aatctgagtt atttggcaac ttaacccaag tacaggttaa
     5401 caatctaaaa agagagataa cagctcgatt aatatcaaaa cttcaataaa actaattatt
     5461 caatcagcca actcgccaag ttatcagatt ggagagatta gttatgtcaa tacaaaagta
     5521 cacaacaaaa agcggtgcac gatatagtgt ccgcttgtat gttcgtaaag atgaaaatgg
     5581 aaagcaagaa tactatgtaa aaaaagggtt taaaactgaa aaagaagcca agttacacga
     5641 agcaagaaaa aaggtcgaga ttgaagatac cggctatcat aagccttcta atgacttgtt
     5701 tataacagtg tatgaagaat ggttgccttc ttataggaat cgtgtacaag aaacgacata
     5761 tcaacgtaca aaagaccttt tccgcttgca tatactacca aaattcggga ataagtccat
     5821 ttctaagatt actcctgttc actgtcagaa tgcagtcaat gaatgggctt taaaattcaa
     5881 gaatatcaaa cagcttaagt cttacagctc tcaaatattt gagtatgcca tttttgcaga
     5941 gctattacac cgaaatccta tggcaaatac aaatctacca aagcgggaaa agaaagaaaa
     6001 tgaaaattac ttttcattag aagaactaaa agctttttgg gatattctca ataaagaaga
     6061 gcctttaaaa catattttaa tcttccagct tttgattact actggtatac gtaaaggaga
     6121 gctagctgct cttcattggt ctgacattta ttttgaagaa cagctaatgt atgttagaaa
     6181 gagctatgca actattcgaa atgaagagaa gaatgcaaga agaaaaactg tcaggattca
     6241 aaaaaataca aaaaatatat cttccgaacg aatattacca attgatgcac aaactattga
     6301 aatgttaaaa atctggaaaa aagaacaagc tcgagagctt ttgcagtttg gcataaatac
     6361 aaatacaaaa aatcaactta tctttaccta tgtaaatgca gacggtgaca ttaatcaacc
     6421 tttgcatgcg gactattcaa ataatatatt gaaacgttta gagaagaaat ataaatttaa
     6481 gcacgttact attcatggat tccggcacac tcacgctact cttctactag aaggtggagc
     6541 aagtatcaaa gaaacacaag atcgattggg tcataaaaat gctgaaacta cccttaacac
     6601 ttattctcat gttactgaaa aagctcaacg aaatgcgtta gaaaaattca ttgcttacac
     6661 tggtttataa tattgtcatt acctttctca ttaccagaac gaaaaaaaga caccttctcg
     6721 caatgagaaa gtgtctgaaa tcgttggtat aacaacgatt aacgttttga aaactgtgaa
     6781 gctttacgtg ctttcttaag acctggtttt ttcttcgtat cattgaataa gtttgaaaaa
     6841 ccttgattta taaaggtttt cccgtaaata tattttatta tatttgatta aatttgaaat
     6901 gtttgtgttc taaaatgtgt tctaatttaa aggcagtata tttcgtattt tagattaaat
     6961 ttttaggctc attcattcta tttagtcaat aaatatttta aaaagacttt ataaacaaag
     7021 atgttcaagc gagcatctta tttttttgtg cttaatttga aaggagaaca ttttatgtat
     7081 aaaaaacaaa aaaagaaatt atgtgtatta actattttga gtttggctgt tcaagcaatt
     7141 gtaggagcaa ttgctcctac gattgctttt gctgatgaaa ttacccatcc acaaacggta
     7201 accgtagaac tagatttagc acataaatat gctgttgaag gaacttttag tgatggtcgt
     7261 ccaatgtcag aagtcactgt tccacattat gctgtgtaca acggcgtgaa acaagatatt
     7321 ttctgtattg agccaggagt cccaattgac aatgagttca cgccgggtta tgagaaaaat
     7381 ccattgcctg atatgccaga gaaagcgaaa ctagtttccg ttctatggaa aaaagccggt
     7441 acagatgtag atactcatat tgtggcacag aagatgatct ggcaagaagt taatgggtat
     7501 acccttcact caatcaaacg attgaatggt agtgccgtaa atatcgcagc cattgaggca
     7561 aaaatcaatc aagccattgc cgattatcag aagaaaccaa ccttccataa tagtacggct
     7621 aaaacagtat tagggcaatc gactactgtg acggatacga ataatctgaa tttgtcagag
     7681 ttcgatgagg ttgtggaaaa taccgcaaac attgattatc gtgtgaatgg taatcagttg
     7741 ataatcaccc caaatgccaa ttcaaaagaa agtggtgtgc tgacgcttaa gaaatctgct
     7801 ggtaccggaa cacctgtcgc ctataaaaag gctggccaac agactttaat ggccggggcg
     7861 attgataaac ctaataccta cacaattaaa attgatgtgg aaacggaggg ttctttaaag
     7921 atcaaaaaag tcgataaaga atcgggtgat gttgtaccag gaacggtttt ccatttagac
     7981 tttggaaaaa ctttacctgc aaaagacgtg actactgaca aagaaggcat tgctacattg
     8041 gatgagattc ctcatggtac aaaagtaacc attaccgaaa aatcagtacc ggcgccttat
     8101 acgattgata ccacccccat gactaccacc attaaagcag gtgaaaccat ttacgtgact
     8161 tcaaaaaacg cacaggaaaa aggtcaaatc attctggata aaagcggagt tgaaacaggg
     8221 agtgatcttt ggaatgacaa ttattcctta gctggtaata cgtttgccat tcgcaaagac
     8281 agtccaaccg gtgaaatcgt tcaagaaatg accacggatg aaaacggtca tgcggaaaca
     8341 ccaaaagaga ttgctaatgc gttggaattg ggaacctatt atgtgaccga aactaaagct
     8401 agtcatgggt tcgtgaatac gttcaaacct ctaaaagtcg aattgaagta tgccaatcaa
     8461 accgtagccc ttgttaccag taacgtaaaa gggcaaaatc aagaagtcac tggcgaaacc
     8521 actttgacga aagaagacaa agatactggg gataaagcac aaggtaaggc agtctttgag
     8581 gggactgaat ataccttgtt tactgctaag gatggaaaag cggttaaatg gagtgaagct
     8641 tttaaaccag aaatggtaaa gggaacgaaa gcttctgatg aaacagtgac tctggcttta
     8701 gatgaaaaga atcaagctgc cgttaaacac ttagccatca acgagtacta ctggcaagaa
     8761 accaaagcac ctgaaggata taccttggat gaaacgaagt atcctgtttc tatcaaaaag
     8821 gtagatgata acgaaaaaaa tgccgtgatt acccgagatg ttacggcaaa agaacagatt
     8881 attcgttttg ggtttgattt cttcaaattt gccggatcag ccgctggcac tgccgaaact
     8941 ggcttcaatg atctgacctt taaagtgtcg ccattggaag ggaccaatga aatcacaggt
     9001 gccgaagatg aagcgaccac agcttataac gagcaattag ggtttgatgg ttatggcaag
     9061 tttgaaaatc ttccttatgg ggattattta cttgaagaag tagaagcccc agaaggtttt
     9121 caaaagatta cgccattaga aatccgttct acttttaagg aaaacaaaga agactttgtg
     9181 aagagtgagt atgtctttac cattaccgaa caagaccaaa aacaaccaat taaaacggtg
     9241 accgttcctt acgagaagct gacgaacaaa gcattttctg ttagtttgaa ccgtttaatg
     9301 ctctatgatt tgcctgagga agaagatagt ttaacttctc ttgcgacttg gaaagacgga
     9361 aacaaagaat taacttctct ggattctacc gagctagttg ataaattgag ttacaatcta
     9421 catgaaatca aagaagactg gtatgtggta gcgcaagcca ttgatgtgga tgcgacaaaa
     9481 gctgcccaag aaaaagatga aaaagccaaa ccagtggtga ttgcagaaac aagcgcaacg
     9541 ttggcgaaca aagagaaaac aggaacttgg aaaattcagc ataaattaac cgccgaacaa
     9601 gttttaaata aaactattgt cttattcaac tatgtgtatg aaaataagga agcttttgaa
     9661 gcaggcgata agccagtagc gaaggatgtt agcttgaata atcaagccca aactgttaat
     9721 tgtacggtgg aacgtcatgt ttcgatccaa acaaaggccc acctagaaaa cggttctcag
     9781 acctttactc acggtgatgt ggtggatatg tttgatgatg tgtcgattac tcatgatgta
     9841 ctggatggct caaaagaagc tttcgaaaca attctgtatg ctttattacc ggatggtaca
     9901 aacaaagaaa tttggaaatc tggcaagatt gagtatgaag taaatgataa agaattcacc
     9961 aaaactgtgc ttgcgaaaaa agtagatacc ggaaaatatc cagaaggaac caagtttact
    10021 tttgcggaaa tcaattacga taaagatggg actatcaatg gaaaacacaa tgaagatttg
    10081 aaagaaaaat ctcaaacctt aacaccaaaa gaagttccaa ccatactaag tacgccgaaa
    10141 caaccggaaa caccaactgt tccaagtggc tctcaagaat ctagtctcac agtgaagaca
    10201 ttccctcaaa ctggggagaa aaattccaat gtgttactgt tcattggttt tactttgatt
    10261 tttgcgacgg cgggctatta tttctggaat cgccggaact aaggtgatga gatgatacaa
    10321 gtaaatccta agaaccaacg tgcgcgaccg cctcctaaaa aagtagtcgc gctatttttg
    10381 tatccaaaac taacaatgaa aagaggaaaa gaagatggaa ttgaaatttg tcgtgccaga
    10441 tatggctgaa acctttggaa agttaagtta tgccggagaa ggcgaagttt taacagaagg
    10501 atatggacgg aatactacgg tgattggtcg cagttatcat ttgtattcca gtaagcaacg
    10561 ggccgatgat attgaggtgg tagtagctgc ggaagccggt gaaaaggatt ttgatcaaga
    10621 ccaaccgcta aaagccgtga atccccattt ggttgccaaa ggctatgaga ttgagaatcg
    10681 tgggtttact gattacgtgt tgtatgttga tgatttagtg aaagcatagg aggaaaagaa
    10741 gatgagatta acagaaggta ttgtcgtaga ttcaggttta acatttggga agttgcgttt
    10801 ttccgcatta cgccgagaag tacggaagca aaatgaggat ggaacaattg gtaatgaggt
    10861 aaaagaacga acctataatt taaaatcctc tgcacaaggg cgaatgattc aagttagtat
    10921 cccggctaat gtacccttac gcgagtttgc ttatgatgca gaggtagagt tggtcaatcc
    10981 aatcgtggat accgttgcca attatgtttt tcgagaagga actaccgtta attggtttat
    11041 taaagcagat gatttagtct tgaagcggca gccgaatcaa ggaaatccta cgaaccaaaa
    11101 tgaaagaaag aaataggtga agattatgga aattaatgtt gaacatattt tagattgtct
    11161 cgatcaatat ggaaaaggtg agctaacgga ggaacaactc actaacgcat taacctatga
    11221 tgaaaaaatg tttctgatta tgcatcaagg gttactggaa gtaggtaacg atgccaaaga
    11281 agactttgat ataccggatt ggttaggaga agaagagtct ttctttatgg ttgtcgaggt
    11341 aaatgaaagt ttatgtcgac aggcagagtc tgtcttggaa gaaatcggtg tggagatgcc
    11401 ggatgccatt gaaggctttt taaaacaatt ggtggaaacg aaacaacttc ccgtagtagt
    11461 aaatgactag cataactttt tgaaattacc caatgaaatc ctattgggct ttttcgactg
    11521 gaggtagaaa tcatcaaaat gtacaaagga catcgcatac gcgcaggaga tcaacacttg
    11581 atttatcact ttgttcttgg ctggctaagt gccttattca tcggttggat gggcgtcttt
    11641 tattttccag agcttagaca atttggtctt tctaaggtat cattttccac tattgaaatc
    11701 gcttggtcca tgaaagattt agtatgtctg cttggaagtc tcgctctttc aggaagtatg
    11761 atgttgcttt atattcattt ttttcctgat cattggcgaa gtttgtggaa tcgacaaaag
    11821 ctagccagaa tgattctaga aaaccattgg tacgaagtga agcaaactca aagtgatggc
    11881 tttttcaaag atttgaatag gagtcgaacc aaagagacta tcagttactt ccccaaagtt
    11941 tattatcgaa tgaaagatgg cttactgtct attcgcgttc aaatttcact gggaaaatat
    12001 caagatcagc tattgaagtt ggaaaagaag ttagaaagcg gactgtattg tgagttagta
    12061 gaaaaggaac tcaaagactc ctatgtggaa tacaccttgt tatacgatat gattgccaat
    12121 cgaattggga tagaagaagt agtggcagaa aatgggacgt tgcgattgat gaaaaatcaa
    12181 gtatgggcgt atgattcctt gcctcatatg ttaatcgctg gtggcacagg tggtgggaag
    12241 acctatttct tactcaccat tattcaagca ctattgaagt cagatgcaga attgtttatc
    12301 ctcgatccca aaaatgcgga cctcgccgat ttaggtacgg taatgcctca tgtctattct
    12361 caaaaagaag aaatttctaa atgtgtggaa gatttttacg aacgcatgat ggctcgtagt
    12421 aggtcaatga aagaaatgcc caactacaaa acaggagaga attatgcgta tctcggactt
    12481 ccgcccaact ttttaatctt tgatgaatac gtggcttata tgggtgcgaa tcgttttcct
    12541 acaagcatcg aatagatgtg aaatggaagc ctattgagat tatcaatagt gacaactaat
    12601 gttttgagag atggaatgaa agccttcaag ttgagctgaa acatccccgc taatactcct
    12661 acatgctcta atcaatgaag tagattggtt ttggtatgaa gctaggtgaa gtcgtctgaa
    12721 atctgcctaa gtgcttgagc ataaggctga ctatagggcg ggcggctgaa aaagataact
    12781 atggtaagaa tgtctgaaaa tgactgacga atctgcgaat gtacgggtct aaaaggctga
    12841 tcttattgaa agataggaac atacggacgt atggtgagat accgacaagt aaggttccat
    12901 gatatgaaaa tcgggatagc taacgaagaa gctataattt cacaggctca tagtagatac
    12961 ctaaggttat acatgatagc taaaaacatt ggaacgatgt aagcagaaaa ctatcaatta
    13021 aatgactact tctagatagg tgcatataag taataacgaa attgctttgt ttctgtgaaa
    13081 gtaagggcac agtaccgatg aagcgtctaa caaatgtgga gggatagccc taagtctatt
    13141 taataacaat gaatgttgaa acagatgaag ttcaaggatc tggtaagaac gtgggaaaat
    13201 ccgttatgga ggtgccacgc ttgaacacaa aaatacgtta ctgggaatat tatgatctac
    13261 aagaaacatt cgatcagcta tttactcaaa gtagaaacgg gaaaaagttt tatcaattat
    13321 atgagttaat catttctgaa aataatattc ttttagcata ccgtactatc aaggcaaaca
    13381 aggggtcttc gacaccagga acggattcct tcacaattga taactataag gaaatgaacc
    13441 aagcagagtt tatccatctt attctcagtc atttagaaaa ttacaaacca aaatccataa
    13501 agcgagtaat gatacctaaa cccaatggtg agaaacgccc attaggaatt ccatgcatga
    13561 ttgatcgaat gatccaacag atgttcaagc agatactgga gccaatatgt gaagctaagt
    13621 tttatgaaca tagctacggt ttcagaccat tgagaagtgc aaagcatgct ctaggtcgca
    13681 ttatgtatct cataaacatc agtaaaatgc actatgcagt agacattgat atcaaaggtt
    13741 tctttgataa tgtaaaccac cgtttactga taaaacaatt atggaatata ggaatttgtg
    13801 acaaacaagt cttagctatt ctttcaaaat ctctgaaatc acccattcaa ggagaaggta
    13861 tcccgagcaa aggaacgatc caaggaggta tcatttctcc actattatct aatgttgttc
    13921 taaacgactt agaccattgg gtctcaaagc agtggcatac ctttgaaaca aagtatccct
    13981 atactaaagg ttacaataaa tttcgtgctc taagagatac aaatctaaag caaggctata
    14041 ttgtcaggta cgcagatgac tttaaaatca tgactaatga ttatccgaca gcattaaaat
    14101 ggttccacgc tgtcaagctt tacctaaaag acagacttaa attagatatt tccaatgaga
    14161 agtctaaaat cgtcaatttg cgtaaatgta agtcggaatt tttaggattc gctatttgcg
    14221 tgaaacaaaa agggaaaaaa tgggtttgta attcacgtat ttctaacaaa aaaaaggatc
    14281 agattaaaga agagattaaa caaagaatca aagatattca aaagagtcct acagcacaaa
    14341 atgctctcct gtttaatgct ctcgtgctcg gtgttcataa ttatttcagg tatgccactc
    14401 atattaattt agatttgaat aggatgaact acgttctttc attcactact tacaaccgat
    14461 tatcaaattg tagtacaaga tgttacccca acaaagcttc cccaacttac aaaaagtttt
    14521 actcaacaac gatgaaaact tataaagtag ctggagtcta tttgtaccct ctttgcgacg
    14581 tatctacaaa aacaatttac ggatttaata cgaaggatac accgtttact ccgtttggtc
    14641 gtcaaagatt ggagcacaaa agcctacagt ctcttgtgtt ccaagaatta agaaaattga
    14701 tggaatcgaa aataccgaat agaacggttg agtatttaga tagtagaata tctcgttact
    14761 caatgaaatc aggcaagtgt gagattacaa aacaattttt gccagcaaaa gccgttcact
    14821 gtcatcacta tttgccaaaa agtcttggtg gagacgacaa attcgataac ttgagaatta
    14881 ttcataagga tatccatctt ctgatccata caacaaataa aatgataatc gatcactacg
    14941 tgcatgaact gaaactggtg ccagagcaaa tagcaaaaat caacctttat cgaaagatgt
    15001 gtaatctgca aaacattcaa tagaaaataa atagatggaa cgccgtatga ggtgaaagtc
    15061 tcacgtacgg tgtggagcgg gggaaaagat ggagattata tcaaattctt acctatcgct
    15121 ataaatgtta acgacgaaag agagtgcggt gattttgaat aagctcaaac aaatcgtgat
    15181 gttaggtcgt caagctggtt tctttctgat tttggcttgt caaagaccag atgcgaaata
    15241 tttaggagat gggattcgcg atcaattcaa ctttcgcgtg gctttgggtc gtatgagtga
    15301 actcggatat tccatgatgt ttggggaggt cgaaaaaaac ttctttatga agcacatcaa
    15361 aggtcgaggt tatgtggata caggtggcag tgtgattagt gagttttata cgccacttgt
    15421 accaaaaggg tatgatttct tagattcaat tagtaattta gaaaagatga atgattgagt
    15481 gatcttcaat actgacttta tacttcaagt aacgtataat acttttatat agtaggaaga
    15541 aaggggataa ttaatatgga aagtaatgat tcttatttag agtatgcaaa agcaaataag
    15601 gataagttta tttcttatat tatcaaaggg aaatccccca aagttggacg agacgcagtt
    15661 tttatggcag ggagtcctgg ggctggaaaa tcagaggttg ccattggatt agctgagaat
    15721 tatgataacc atgtgattat tgatgcggat tactttcgca cccagtttcc tgattataat
    15781 ggaaaaaact ctagtctctt tcaaaaagca tcttcttggt tggtagagta ggcatttaga
    15841 tatgtcttag atcatggcta ttcttttatt ctggatgcaa cttttgcaat attaagtgct
    15901 gaaaaaaata tctcgcgggc gttgaaaaaa cattttcgag tgacgatttt ctacgtttat
    15961 caaggcccta aaatcgcttg gaaatttaca caagaacgtg aaattgcaga gggacgtcat
    16021 gtgccgaaaa aggcatttat taacgctttt ttcaaatcaa gagagaacat tgcaaaagta
    16081 aagagtaaac atccagatgt attgcttcat atcataataa aagattatac gaataatatt
    16141 tccgaagttc attatgctac ggataatcta gaattatttt taccactaga gtatagtagg
    16201 aaagatttgg aggttcaatt acatgactga gaaagaacaa gtaacgaaaa ttgtaaagaa
    16261 atacaataag agcattgcgg atttatcgga aaatgctact gcaaaagagt tcaaaacggt
    16321 gattaaatat gtagccgatc aggccaatga aaaacaacgg aaattagttg gattaaataa
    16381 aaaatagtag gacaaattaa taatgtatga gccaagagca aaatgttctt ggtttttttc
    16441 tttgcaatga atgaggtaat gtatgagatt tttagattta ttcgccggta ttggtggctt
    16501 tcgtttaggt atggaacagg cgagccatca ttgtatcggt ttttgtgaaa ttgataaatt
    16561 tgctagacgt agttataaag caattcatga tacaagtaag gaggtggaaa tgcatgacat
    16621 cacaagtgta tcagatgagt ttattcaatc cctcggacca gtggacatcc tttgcggcgg
    16681 atttccgtgc gtgcgatgcg ttcctaattg aaaagattag gcactgatgt atttaagaac
    16741 gcgaaagtgt tcagttcagt agaactgatg atttaaatgg tggaatgaag gagtaacgcc
    16801 ctgaaacgcc taccctgagg ttacgctgtg cagaatcgtg ggtaatcatg atgatgtgca
    16861 aagcgcgttg aagtcggcag agagttaccc aaacaaagag aaatgttctc aggtgtggaa
    16921 agtcctccag agatggagga tgtagcctat atgccggggg tctataaact atctatggtt
    16981 agtatgtaat ggcaattact agacgaatgt ccgtatgtac gggtctagag aacgcagata
    17041 atagaaatgt tatcaccact acatgtgggt tgtgtgaggt ctagtaaaat tgcacggtat
    17101 gaaaaacctt gtaacgttat aggcgttacc aagcacacag gctcagaggg caaacctaag
    17161 gataaaattg aacagattga atcatcggaa cgcagtaagg tcaggacgtg gagtgattac
    17221 acacgaagaa gcggtaatag ggaaaacgat tttgtataac ctatttttat ctgaaggaaa
    17281 gcggagccac agtaccgatg aagtcatgga aacatgatgg agggataggc tctagtcaat
    17341 gagagacgat aaccattcaa cacacgaatt ttcaaaggtt cgagtacgac taagaaaggc
    17401 aaagtattct ataaaaagga gaactgccga ctttgacgac tgagaaaata atgaaaaagc
    17461 agaaactaag aaacgctgaa tattatgact ttcagactat tcaggatcag ctttatagtc
    17521 aaagcaagca tggaaaaata ttcaagcact tgatggatat catttcatcc gaccagaata
    17581 taaaacttgc ttatcgaaac atcaaaaaga atagtggaag tcacactcca ggcgtagatg
    17641 gaaaaaatat cgagaacctt gccaagtggc aagacgatgc attaatcaag catgtgaaac
    17701 ggaagtttca atggtacgaa ccacaggcaa tacgcagagt tcaaatacca aaatcaagtg
    17761 actctaccaa aacaagacca cttggaatac ctaccattat ggatagactt atccaacagt
    17821 gcattcttca agttctagaa ccaatatgtg aagcgaaatt ctatgacaaa agtaatggat
    17881 ttcgtccaaa ccgtagttgc gagcacgcac tcgcgcaagc tgagaaaaat atgcagttat
    17941 caaaattaca ctttgtcgtg gacatcgata tcaaaggatt ctttgacaat gttaatcatg
    18001 gaaaactgat aaagcagtta tggacattgg gcatcagaga caaaaaattg atatgtattc
    18061 tttcagcgat gctaaaagca gaaatcgctg gtgttggctt tccagaaaaa gggtcaccac
    18121 aaggctcaat tatttcacca ttattatcaa acgttgtttt aaacgagcta gactggtgga
    18181 tttcaagtca atgggaaaca attccgacaa aaaaggaata caagcaatgc gatggtcatt
    18241 caagtgggcg gctacaagcg ctcaaaaaaa ctaatctaaa ggaatgtcga ttagtacgct
    18301 atgcagatga ttttaagatt ttttgtcgaa caagaagtga cgctgaaaaa atctttgtag
    18361 ctactgaaaa atggctaaaa gagaggttag gtttaaatat cagccctgaa aaatccagaa
    18421 tagtgaatct caaaaagcat tattcggaat ttctaggctt caggttgaaa gtggttagaa
    18481 aagggaaata tcctaacggg aatccccgtt ttgtcataag gtcacatatc tcaaaaaaag
    18541 cattgaaaaa tatacatctc agtgcgaaaa aacgaattag taatctgcag aatttatccg
    18601 atgacagaga atgttataaa tcagttcaaa attataacat ctttgtttta ggaatacaca
    18661 attactattc caaagctaca catgctagtc tagattttgc aaaaattgcc tttcctatca
    18721 gaaagagcct aaaaggaaaa ctacaaggaa acttctcgaa agaaaaagga atatgcagca
    18781 gcttaattga aaacagatac ggaaaaagca gacaattagc ttatgtctat ggattcgctt
    18841 ttatccccat tggctatgtt caacatcggg ccccattaga taaaaagcga gaaatcaatc
    18901 agtacacggc cgaaggtaga gagttgattc ataaacaatt aggaagctca atcaatccag
    18961 acttactgag ttacctaatg gaaaacccaa tcaaaggtaa aagtatcgaa tataacgata
    19021 atcgattatc acttttctgt gcgcaaaaag gaagatgtgc gatcacaggt gatgacctta
    19081 ccattgggaa tatgcactgt caccacaaac tatctttgaa gttaggtgga acagatgaat
    19141 acaaaaactt agcttttgta acggttgaaa ttcatagact actacatgcg acaaatctga
    19201 aaataatcat tgagttagtg cagaaactga atttatcgag aacgcaactt gagaaagtaa
    19261 acaagttacg aaagaaaatt gctcttccaa tcattgaata attttcaaag ttgaaatcgt
    19321 gtatatgaat gtaaaagaat ttcgttgatg gaaagccgtg tgaggtgaaa gtctcatgca
    19381 cggtttgaag cggggaaaaa tctagagata atttcaaagg attacctatc gcaatcaagc
    19441 tttttcaatt gcgggaaagc ggcaaggatt ttccgatact cgaggtactt tattctttga
    19501 aatcgctcgg ttcgccactc ttctccgacc taagctttta ttccttgaga acgtccgagg
    19561 attgctcaat cacgaaggag gggctacgtt cgagacgatc ctccgaacgc tggatggatt
    19621 ggggtatgac gtggagtggt aggtgcttaa ctcaaaggcc tacattcccc caaagccggg
    19681 agcggatatt ccttatcgga cattctcgag acgcatgtac cgaacaagta tttcctatca
    19741 ttggaccgtc ttcaacatct gatcaaaaca tccgaaattt ggtaaatatc aatccttcca
    19801 atcgaggaat gggtggacaa gtctatggtt cagatggcgt agcgccaacc ttaacaggtg
    19861 atgaaggaat caaaatcgca ctgccagcaa gggatggaat ctctgtagca ggactattac
    19921 cgggaaagtt tgaacaaggt aatcgagttt atgaagttac tggaaccgcc cccaccttgt
    19981 ccacgaaaca aggtggtaca aaaatcatga ttcgaaaaaa tgaaacagaa tatcaagagg
    20041 tgaagccagg agacagtgtg aacctgactt ttcctaattc cacaagtcgc cgaggtcgtc
    20101 tgggaaaaca gagtgttcat accttactaa caggagacca acaagcgatt gtcatggatc
    20161 agtatcaaat tcggaagcta acacctagag agtgttggcg acttcagggc tttcccgatt
    20221 gggcttttga tcgagcttct caagtcaatt cagatagtca gttgtataag caagccggaa
    20281 actctgtaac agttccggtc atttttgata ttgccagaag attaaaggag gtacaaaagt
    20341 gtgatttatg atgagttggt tggagaaatt tattgggtga ttggaaagat tcagtcagac
    20401 ccagaattag aagaacaatt gcgacggtta aactttgata ttcgcaagaa tggtgtcaaa
    20461 gtcgctggcg atccatattt aatgaatgag gagacggatg ctcgaattga aataaaccaa
    20521 gtaattgcag aatttgagcg gattgcggac caagcaaaag aaccggatat tcgtcaatac
    20581 ttgttagaaa tgaaagcaga gttggaagta aatggtatta cagatgagta acctaaacca
    20641 acacaactga ctgttaggcg ctgaccgcgg aggattcgcg aagcagaaat cctccgtggt
    20701 gctgtgcctg ttcatggacg gcgttagccg gccatgatgt cttacccccc gtatctaaca
    20761 ggggggtaca aaaacacaaa aaacaagcaa gcagcgatta agaatggttg tcatgacgag
    20821 gatagttagc tgtttgaaca ggtgttaact cattttgaaa agttaacacc ttatttggag
    20881 gtgaaaactt ggtacaacag aacttagatt atcgtttact aaaagatcgg cgcaatcaat
    20941 atggtgtttc tcaaaataaa ttggctactg cttgtggatt gagtcgcccc tatttgaatc
    21001 aaatcgaaaa tggtggcgta actacatcta caaaaactat gagaaaaatt tttgaccaac
    21061 tggaaagttt caatcccgat ttgcctttga cgttactgtt tgattatgta aggattcgtt
    21121 ttccgacaac ggatgttcaa aaaattgttc aagagatttt gcatttgaaa tttgactata
    21181 tgcttcatga agactatgcc ttttattcgt accaagaaca atacatcatg ggtgatattg
    21241 tggtgatgct ttctcatgag gaagagaaag gcgttctttt agaattaaaa ggtcgtggtt
    21301 gtcggcagtt tgaaactttt ttgctcgctc aaaagcgtag ctggtacgac tttttcgatg
    21361 attgcttaaa agctggaggc gtcatgaaac gcttggattt ggcaatcaat gatcgagttg
    21421 gcttattgga tattcctgag ttgacgaaaa aatgtcagaa ggaagaatgt atctccttat
    21481 tccggacttt taagagttac cgctcgggtg aacttttgaa agctgatgaa aaggatggaa
    21541 tgggaaatac tttgtatatc ggaagcctta agagtgaggt ttatttttgt ttatacgaaa
    21601 aagactatga acaatatatc aaattgggaa ttccgttaga caagagcgaa acgaagaatc
    21661 gctttgaaat tcgattgaaa aatgaccgtg cctaccatgc aatacaagac ttgttaaaag
    21721 gtcgaagtat tgagggtacc acattttcca ttatcaaccg ttacttacgg ttcacggata
    21781 aagttgaagg gaaacggcga acaaactggc cgctaaatga acgatggggt cgctttatcg
    21841 ggcgaaatcg caaggaaatt caactgactt ctgaccccaa accttatact atcgagcgaa
    21901 cactgaactg gctaggtcga caggtagcac ctacgtggaa gatggctaag gagttagatc
    21961 ggttaaatca gacgacttat attcttgata tggtacagaa tgctcagcta tcggatcggc
    22021 ataaaaagat tttggaacaa caaagtatgg caatagaaaa cttgattata tgaaaagagg
    22081 aagcagtatg cttggtgtta ccgtgatgtg cttaattcat acaagtgcgg ctgcagatcg
    22141 agtaatggaa aggaaagtga taaaatgaat ttcggacaaa attt
//
LOCUS       DACGLE010000042        21457 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0534
            SAMEA3304449-rid9560453.denovo.043, whole genome shotgun sequence.
ACCESSION   DACGLE010000042 DACGLE010000000
VERSION     DACGLE010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304449
            Sequence Read Archive: ERR985064
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21457)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21457)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702225.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702225.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 20:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,045
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 27 of 67
            Pseudo Genes (incomplete)         :: 25 of 67
            Pseudo Genes (internal stop)      :: 19 of 67
            Pseudo Genes (multiple problems)  :: 4 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21457
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304449-rid9560453.denovo.043"
                     /strain="VREN0534"
                     /db_xref="taxon:1352"
     gene            5..403
                     /locus_tag="IXT20_001717"
     CDS             5..403
                     /locus_tag="IXT20_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HAQ4860158.1"
                     /translation="MTIFETVVTDVGIEAEVFRKEKIMILFGDEAPETLTDYCYKIEL
                     NQVRKEITCDMKLCFDKQMYVITAVGDVVQTNLNELGHITIKFDGSHEPELPGTMYVE
                     DKEMPHLRKGTIIKIDQEDQNGGKLYGNHA"
     gene            387..1082
                     /locus_tag="IXT20_001718"
     CDS             387..1082
                     /locus_tag="IXT20_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HAQ4860159.1"
                     /translation="MEIMLDTANTKSIYSCQQFLNLTGVTTNPTILKKEGASNFFSHM
                     KEIKKIIGQIPIHIQTIGRTEEEMIEDARVIIEELGKDTFIKVPVNQAGLQAIKKLKK
                     EGYLITGTAIYTELQGYLAINNGADYIAPYYNRMQNQGIDAMKVIDSLRTEIERTQRP
                     TKILAASFKNCEQVTTAIRSGAHAITLGKELLDQVLEMPSIDKAVRGFQEDWVQTFQA
                     ETIGELKKVADYF"
     gene            complement(1676..2563)
                     /locus_tag="IXT20_001719"
     CDS             complement(1676..2563)
                     /locus_tag="IXT20_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rotamase"
                     /protein_id="HAQ4860160.1"
                     /translation="MGNFLKKQLLIMCLSACAILLFSGCSKSNETIATFKGGTLTVQD
                     FYDNPKVKSNNQTLLKKMIVFKAFDDVYGKEISSKEVTKEYNEQIKKLGPNYKEQLKA
                     VGQTEETYKKLFKQMLAFQYGLKANVKLTDKDLDTAWKEFYPEVSTQIILFSTKEEAD
                     KAKKEANEGENFSKLVQAYGKNKTLKETDGKMNFDSTNPEIPTEVKKAAFKLKNGEVS
                     DIIPVTDPTTYQQSYYLVKMVKKQDKGSNKDKYKSELEKIATEAKTLDTEFMDKTIRK
                     VMKKDNVTIKDPYVKNIFK"
     gene            2748..3587
                     /locus_tag="IXT20_001720"
     CDS             2748..3587
                     /locus_tag="IXT20_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HAQ4860161.1"
                     /translation="MIRIIRLIGSVLIVLVGFVLGILVNNALADMFIGNSEWIGAIAG
                     TVGHLAVFLLALFLASKVEGKRIEDYGVSVRGKDWGYLAGGLVVGIGVFLLITSPLYL
                     TGAYRLDSGNANIVPLITSFILFIAVGASEELLFRGFFQHQMLRFGPLIAMIGSAALF
                     ALLHGLNPNMTFLAVFNIFLAGCFFSALIYSTGSLFTAIGAHITWNWTQGAILGIPVS
                     GTQEQGFFSTLIQSQNTIITGGNFGAEASISTTLFLLVLIVGLLLYAYKTKRMKKYTA
                     VYQ"
     gene            3783..4757
                     /gene="sgrA"
                     /locus_tag="IXT20_001721"
     CDS             3783..4757
                     /gene="sgrA"
                     /locus_tag="IXT20_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored fibrinogen/nidogen-binding
                     adhesin SgrA"
                     /protein_id="HAQ4860162.1"
                     /translation="MKKTATVLFVILGLSIIAAMIGISTSANERANENLSFTVKTDRI
                     VYDMTQQVITIPVKPNKSVNASDVHAVLTYGWDGNGSSEKVIGEVYLKDVQWTAGIEY
                     TIMISAELSIDEIKSKDKVDLIVFYDGQMTITENLKPSSWTVVGPPSSTDSTSSESST
                     ENTSGESSTESTSSESSTENTSSESSTESTSSESSTGSTSSESSTESTSSESSTGSMS
                     SESSTGSTSSESSTGKVNNELDLVPIIPTKEQKRNAGSNLKSVLKQPDFVSRGIDHQE
                     KAKETDNRKSDLPETGSRTLNKLITWMGVLLILIVGASYFRSLFHRVK"
     gene            5055..5411
                     /locus_tag="IXT20_001722"
     CDS             5055..5411
                     /locus_tag="IXT20_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4860163.1"
                     /translation="MFEEFIDINERQVYQFLNYCYERDEKLYVVKDIALDLNYTLAKM
                     NSVIQQAESFCERYPEYKLSFLSENKMIKVEFSSQFLLSKVYSILLEGTIGYILLDSL
                     YKGTYQSLENLSQKII"
     gene            5414..6571
                     /locus_tag="IXT20_001723"
     CDS             5414..6571
                     /locus_tag="IXT20_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4860164.1"
                     /translation="MRTIQRKLRELNTILKNYHLSCSLKKGNPIEGEEYRIRYFFHLM
                     YWQSFDENHSDILSSIKNKRQLLEKFAKHAPYTRSIDRKKWISLLRISLQRMKQGHSI
                     TYVPEEMNKFVHPLITKEEFTHYILEPFFEANFWSMNHITPTEQAYLYFMFGVMNTYL
                     EEDLLDTPDKVQESWGWIESSIVENFEKNFKICFTTSEKNYLYVNLAMIHLYSRVFGT
                     KKKVDAFGKSAEDRDFQHIFPVMYPIMQQFFSGVARRIPELNTYYEKNRRLIFQYCML
                     VRDIFIKHEQPVIFYIQSKYGKTQELWAKKRILSMTERPIKYSASADQLPDIVISDYP
                     IDKKAYDQTNTQIFYWNGQPTKNDWARLKAEIIKIRNEKNRSILEKLDFVN"
     gene            complement(6597..7037)
                     /locus_tag="IXT20_001724"
     CDS             complement(6597..7037)
                     /locus_tag="IXT20_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HAQ4860165.1"
                     /translation="MMIKLYTGSGCSSSRRAKRWLIVHDIPFIEQNLIRQPLTKNEFI
                     HLLKLTNNGVTEMLSTHSISYKNLVHRIDELSLSELYIEIRTSPSLLKKPIIYDQSAK
                     LLTGFSEDKIRMFLSKTKRKQELNELLLSGHLVDFKENNLFAMS"
     gene            7197..7703
                     /locus_tag="IXT20_001725"
     CDS             7197..7703
                     /locus_tag="IXT20_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAQ4860166.1"
                     /translation="MSNTADAIVVIDLQKGVCFDSSPIHELETVTALVNKRIKTYETI
                     GKPVIFVQHEDAQLVPQTKPWELLSELSIPEHSYFVRKTHANSFYKTNLSVLLTELDC
                     HSIELCGAQAEYCIDTTVKFAHGLGYQLMMCRGATTTYDNPFMKAKETIAFYEKIWDK
                     RFLTFLEQ"
     gene            7871..9784
                     /locus_tag="IXT20_001726"
     CDS             7871..9784
                     /locus_tag="IXT20_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HAQ4860167.1"
                     /translation="MNSLRTKQVLKGALVILFAVSTSVPQWTVYGESISSESESREVE
                     TTETTDAGNETKSSTSQTETNTETTVETSVTSTDQETTATTDNQQTEEATATKMSNDE
                     SSSAKVSDKENGTENDDIGKGENFNGKEYLNYFGKQMTEILNGPTEDIKSKLIELLVT
                     ELAVNDYGVDKEIIKHLIATYLLYDIAFAKNSVLMSMLGGTDDGTTPTENTGFYPNTK
                     QLHQTVLNKQDGKMMDVYAYYVDQGSDKTVMIHGGFRGNWNNGIVTEEYNDFYKAGYN
                     LLFVDSRATGNSGGDYVTYGQYESDDVLYWINQEVRERPSQKILLYGGSMGAATMMSV
                     LAKDIPVNVKGIIENCGFASIDEQLRFTYSQTVAPALPDAIKNQLDIIGDQEHEDLFM
                     GLLKQYYFDQEMHLDTKAALPTIGMSDSLPKLIIHGTADDVVPVSNAQKLYELSGGYK
                     DLLLVEGAGHGKAQEVDHAAYTKHVTDFLKVVFHDQINVKYVDENNKSLLKDQDEIQL
                     YGAYGENYMTEQKTFEGYELANVEGPTEGIFNETIPTIIFYYKKIPVAPPKKQDPTEN
                     ADNKGKNEPREDQSSDDTQPIQMTVKVQKKTDAKKRMLPKTGEKKHSLLIYSGGFLSV
                     STIITWIWRRKRG"
     gene            9789..10790
                     /locus_tag="IXT20_001727"
     CDS             9789..10790
                     /locus_tag="IXT20_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAQ4860168.1"
                     /translation="MKWFKRIVIAVPVIIVVILVTVFLINQITPKPISLLVRKQFDTS
                     EKEQVYARPDDFQQKIEQIDIKKEISYPSKYKNNNMDIYTPKVEKKKLPILFWMHGGA
                     YVGGDKNDCRDYLEYLCSDTQQIIVNIDYERSPEAKHPTPVIQLNEAIQAAKKEIKDQ
                     ADWSNISIGGNSAGAQISGEYLLALQNEEIKKADHLDPTLENKQITKFISLSGLLDPS
                     ALTQVSDKISSFLYEKCGWAYFDNKDFEQSEKVKSLALVRHADRWTQNTFLTDGNTNT
                     FTKQMEEVTSVFEKAGVKVTKVDYAEKNAVLNHEYQFDFSNKQAQETYQKLVMFFKE"
     gene            complement(10992..11198)
                     /locus_tag="IXT20_001728"
     CDS             complement(10992..11198)
                     /locus_tag="IXT20_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3955 domain-containing protein"
                     /protein_id="HAQ4860169.1"
                     /translation="MKAIHISLLFFASALILFAIHLCSQPYINNNGILVEPAFFCLPL
                     GFLSLLASAGFGFVAFFKRSKQQI"
     gene            complement(11844..12692)
                     /locus_tag="IXT20_001729"
     CDS             complement(11844..12692)
                     /locus_tag="IXT20_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAQ4860170.1"
                     /translation="MSVSIRLQIKTMYNDFSPKEQAIADYILENPSKVSHSPISNLAS
                     ELGIADSTFFQFTKKLGYNGFKDFKMAMLIQENDLSAISIHENIQKTDTELTMAQKVF
                     ESSISTLNDTKKLLQQKDLKKAATIINESKRVYFFGVGGSEIVATDAYHKFLRSPIAT
                     SHSTDYHIQLMEASLLTEKDCAVLISHTGQSKETIHIAETVRKTGAKTIVITSQANSS
                     LAKLGDVVFISISEETEFRSEALASRISQLSILDSLYVILMFYNEKESRRSIAKVRHT
                     ISEIKE"
     gene            12856..13758
                     /gene="gnd"
                     /locus_tag="IXT20_001730"
     CDS             12856..13758
                     /gene="gnd"
                     /locus_tag="IXT20_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating 6-phosphogluconate
                     dehydrogenase"
                     /protein_id="HAQ4860171.1"
                     /translation="MEIGVIGLGKMGLNLALNLKEHHHKVQGLDISSTAVSQARENDI
                     PAYQEISEFLATFEQKKIIWLMLPAGEITENMLRKLFPKLGPGDIIIEGGNSYYKDSI
                     RRAEEFQKNEIGYLDCGTSGGIEGARHQACLMIGGDKETFLSVEQLFKDVAIERGYLY
                     AGASGSGHFLKMIHNGIEYGMMQAIGEGFQLVQKSEYAFDLEQVARVWNHGSVIRGWL
                     MEIMEDQLNEHPNLSNYRGIVSASGEAKWTVETALEMAVPVPVIATSLFMRNLSQEED
                     SFSAKVVSALRNGFGGHEIVEKGE"
     gene            13760..14761
                     /locus_tag="IXT20_001731"
     CDS             13760..14761
                     /locus_tag="IXT20_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HAQ4860172.1"
                     /translation="MPDYHIAIVNSSSFGRVFPHHLERLKSVGDVHFFTVDQEIPGKE
                     LAELLKGFNIIIASVTPFFRRDFFENKDELLLISRHGIGYNNIDLKAAKEHQTIVSII
                     PALVERNAVAENNITNLLSMLRRTTQAQARVKDDRWEDRAEFVGRTLFNKTVGVIGAG
                     NTGSGVIEILRNGFRCDVLAYDPYKSDLYIESFGAKKVELQELLEQSDIICLCANLTE
                     ENYHMISDKEVDQMKENVYLSNSARGALVDEEAMINGLKSGKIAGYATDVLEEEPGRQ
                     DHPYLAFEQVVITPHISAYTRECLEAMGEKCVRDVEQISQHLLPERSVQTKSNYIKG"
     gene            14758..15042
                     /locus_tag="IXT20_001732"
     CDS             14758..15042
                     /locus_tag="IXT20_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HAQ4860173.1"
                     /translation="MMKKMVNGLKVKTGPQFYLYEEGGISKVSDLLKSYGAKRVLVTH
                     GTVSWEKALPKLVFLNDETIQFFYHRYSGECSYAEARRIATIIKKMKSIS"
     gene            15075..15872
                     /locus_tag="IXT20_001733"
     CDS             15075..15872
                     /locus_tag="IXT20_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HAQ4860174.1"
                     /translation="MGYSANLARIDFGVIPTLASNCAPWTPLAVMYKENGQAEGKTEH
                     FYRQAAFFITDPQLVIDSPVPYFIAGLADTLAKWYESELILEQDFLQDESFLKLAQDT
                     AKICKEEILNHSAKAIQDMAQRKLTSEFKQLSEIIFAVAGLVGGLGDKYARNVAAHAM
                     HDAISKYIPESHRFLHGEKVAYGIFYQLALEGRWEKIDELYPLYRELELPVSLEQMGL
                     FPKDKQIIDKMAEFIDSKEKVHLIPIEVTQKSLIQSMMELEGYIRKI"
     gene            15886..16719
                     /locus_tag="IXT20_001734"
     CDS             15886..16719
                     /locus_tag="IXT20_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ4860175.1"
                     /translation="METLSVVQSLLIALWVAAVMSRWLGGGATLTLRFSPLMTGLVVG
                     LIMGEVAQAMIVTAALQMIYMGVFSPGGSMPAEPSIAAAIAVPVALLGDLSPEAAIAV
                     AVPVGLLGSYLYQFRFFINTFLGKYTDKAVEDLNTKAMARSIILYPTVASFLLFVPLV
                     FIALYLGAPLIADVIKALEGTVVIHILEVVGGGLAAIGIATTVYVIGRKDYLVFFFLA
                     YFMSVVLKSLDITMVTYAIFGIIIALIFVQSQKGKQVQPATSNNNSASFDDEDDYDDG
                     F"
     gene            16737..17594
                     /locus_tag="IXT20_001735"
     CDS             16737..17594
                     /locus_tag="IXT20_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAQ4860176.1"
                     /translation="MAEIMDNQTGAPEEITKKDVTKAYLRWHFANEIPHSFERYLAPS
                     LLYGMMPILKKLYKDEDSLKAAYKRQLLFFNTQLSWGGGVITGLMSSMEQQRAEEEYE
                     EKEILMQDDLMYNTKAGLMGALAGIGDAIDSGTVQYIFIAIAVPWAQQGSPLGALFPF
                     ICFGLYQVLLGVFFARQAFTMGRNATGLKQNTGVQTAIEMLSVLGLFMMGVLAGNYVK
                     VESSLKFAISGREFVIQEILDQIVPGILPLAVVMGVYLFYTKKGLKVTQALLWLTGIL
                     IVLAGIGIL"
     gene            17616..18125
                     /locus_tag="IXT20_001736"
     CDS             17616..18125
                     /locus_tag="IXT20_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ4860177.1"
                     /translation="MGVVNLARVDERLIHGQVMVTLSQKSGVNSIFVVDEVVAKDKFM
                     RDLYKNAGSRTGQKTIVITPEKAKFYWDEYQFKEYNCILIAKTVQTIYDLVKHGIPME
                     ELNIGGIAQKNPDTDLLVTKSVYLNKSDAEKLKEMNEQYGVKNIYFQATPSAPKTGLK
                     DVLNKFDLS"
     gene            18162..18563
                     /locus_tag="IXT20_001737"
     CDS             18162..18563
                     /locus_tag="IXT20_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4860178.1"
                     /translation="MKKNELFRDWEFRYRYIYRKRRTKKSKQRFLSALVSDIYSMRTD
                     VTVIAYDTLAYRSKNIYVGDIEKAEKVICTYYDTPVHALGSYFMFDWKDQRKKTIYSI
                     LLSFILLFSLGWWGMMIYNKNPHHVFDLLSV"
     gene            18675..19028
                     /locus_tag="IXT20_001738"
     CDS             18675..19028
                     /locus_tag="IXT20_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4860179.1"
                     /translation="MLEMIRTIDDPSVAYAFVDEGCYGEKGLDSVRSGMKKEAILFYL
                     DSVGADTPLQFSGNYFSNKEQWLKQVDKLKEKNVNYIFSARKKQAQFFYLTKTDLRGK
                     TFNWQNANQIIALFR"
     gene            19062..19505
                     /locus_tag="IXT20_001739"
     CDS             19062..19505
                     /locus_tag="IXT20_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HAQ4860180.1"
                     /translation="MLEIMIVTHGKLSEGLKDAAEVIVGTSSHIETMCLESGEDIQTF
                     GEKVKEKLTMINTAEPMLVFVDLISASPYNQALLAVNELTEEKKAATYVIGGVNLPML
                     LEAINHQMLHTPIEEAVEQIVKQGKDNLTVWHASAEEKKLDEDDF"
     gene            19522..20607
                     /gene="uxuA"
                     /locus_tag="IXT20_001740"
     CDS             19522..20607
                     /gene="uxuA"
                     /locus_tag="IXT20_001740"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HAQ4860181.1"
                     /translation="MKWGFRWYGEKGDSIPLKYVRQIPGINGIIGTLLTKMPGDVWEI
                     SEIEELKASVESQGLELLGIESVAIHDAIKAGTDERDYYIDNYIRTIKNLSRCGVRLI
                     CYSFKPIFGWAKTDLFNENNDGSYSLLYDQKIVDQMEPGEMYQLIHSQSKGFKLPGWE
                     EERLKKFAQLEKLYEGMTEEELFENLTYFLEKVIPVCEKADVKMAIHPDDPPWELFGF
                     PRVTKNLGDLKKILNAVDSPYNGLTLCTGSLGANPENDLVEIIHEVGSRIHFVHFRNV
                     EFLGERKFKETAHPSTEGSLDMYHIMKALVQEGFDGPIRPDHGRTVWGEVAMPGYGLY
                     DRAMGLTYMQGLYEAISKEKPLGEHYE"
     gene            20600..21313
                     /locus_tag="IXT20_001741"
     CDS             20600..21313
                     /locus_tag="IXT20_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAQ4860182.1"
                     /translation="MNKIKLFIFDMDGLLLETGRLAYRAYVKSAQKYDYEMRKEVYYL
                     LTGQTEMAIREQMGILYGEDVPYIKWREAINQYKEKIVKEDKRVYTKKGAEEILSFAK
                     ERGIHTIVASSNTRVKVEMYLKMENLYALFDQIISGDDVKKGKPEPEIFLKACSKMNI
                     PPSEALVFEDSIAGIEAARRAGILSFLIKDHLIGLPDHNGKHKLKVDVSLYESNKADY
                     EFYDLHQAKRFLESKGLYL"
BASE COUNT         7386 a         3293 c         4476 g         6302 t
ORIGIN      
        1 agagatgacg atctttgaaa cggttgtaac agatgttggt atagaagcag aagtattcag
       61 gaaagaaaaa ataatgattt tatttggaga tgaagcaccg gaaacattaa cagattattg
      121 ttataagatt gaattgaatc aggtaagaaa agaaataact tgtgatatga agctctgttt
      181 tgataaacaa atgtatgtaa tcacagcagt aggagatgtt gtccaaacta atttgaatga
      241 attaggacat atcacgataa aatttgatgg atctcatgag ccggaacttc caggaacaat
      301 gtatgtagaa gataaggaaa tgccgcattt aagaaagggg acaatcatta aaatcgatca
      361 agaggatcag aatgggggaa agctatatgg aaatcatgct tgatacagca aatacaaaaa
      421 gtatttattc ttgtcaacag tttttgaatt taacaggcgt aacaacaaat cctacaattc
      481 tgaaaaaaga aggagcgtca aacttcttct cgcatatgaa agaaatcaaa aaaataatcg
      541 gacagattcc tatccatatt cagacaatag gaagaacgga agaagaaatg atagaggatg
      601 ctcgagttat catagaagaa ctgggaaaag atacgtttat caaggtgccg gtgaatcaag
      661 caggccttca agcgatcaaa aagttgaaaa aagaaggtta tttgattacc ggtacagcta
      721 tttacacaga attacaaggg tatttagcta ttaataatgg agcagattat attgcacctt
      781 attataacag gatgcaaaat caaggcattg atgctatgaa agtgattgat tcgttgcgga
      841 ctgagatcga acgtacgcaa agaccaacaa aaatattagc agctagtttc aaaaattgtg
      901 aacaagtcac tacagccatt cgatctggag ctcatgcgat aacgttaggt aaagagctac
      961 tagatcaagt attagagatg ccctcgattg ataaagcagt gaggggcttc caagaagatt
     1021 gggtgcaaac tttccaagca gaaacgattg gtgaactaaa aaaggtcgct gattacttct
     1081 gaatcaattt ctccataaaa taaacgatag ccttctcgaa aactcgagga ggctatctga
     1141 cgattcaata ttcattaatt taaagtttgg gtgactatta ctaactatta cttatttctc
     1201 atttaaacga tgtattccct aacctctaat acatccttgt aactctgaag ttacgccagt
     1261 tttgaataaa tttcataaaa acaaatgaaa agtgtcgcat aaaaatatct ttcgtgtttc
     1321 tctagtataa cagaataaga agaacaagtc cagaagggat atttttagtc ttttttaaaa
     1381 taacttttca gtcgagtttg agtttatgat agttgttttt gaagcgatac ggcaagtgaa
     1441 aaaataggga agcatgaaag aatctactta atggcgcatt gattcatgtg atagaaataa
     1501 aaaacttttt taaattatat cctcatgatt gtaacaaatc cagtgatttg ttttggtaca
     1561 aataaaaaaa ggccccgatg aagttgtggc aaaagtggtt tgcctcaaaa ataagaagga
     1621 atttccaaaa atcacttctc aaattttgcc acatccccaa ttgggtcaaa tttgtttatt
     1681 tgaatatatt ttttacataa ggatctttaa tagttacatt gtcttttttc atcactttac
     1741 ggattgtttt atccatgaat tccgtatcaa gagtttttgc ttcggtagca attttttcta
     1801 gctcagattt gtatttgtct ttattagaac ctttatcctg tttttttacc atttttacta
     1861 gataatagct ttgctgataa gtcgttggat ctgttacagg aatgatatca gaaacttccc
     1921 cgttttttag tttaaatgct gcttttttta cttcagtagg aatctctgga ttggttgaat
     1981 caaaattcat ttttccatca gtttccttta atgttttatt ttttccataa gcttgaacaa
     2041 gcttggagaa attttctcct tcatttgctt ctttttttgc tttatctgct tcttctttag
     2101 tcgaaaatag aatgatctgt gtactaactt ctggatagaa ttctttccaa gcagtgtcta
     2161 gatctttatc tgttaatttt acattggctt tcaaaccgta ttggaaagcc agcatctgtt
     2221 taaagagttt cttatatgtc tcttctgttt ggccaactgc ttttaattgt tccttgtagt
     2281 ttggaccgag ttttttgatt tgttcattat attcttttgt tacttccttt gaagaaatct
     2341 cttttccgta tacatcatca aatgctttga agacaatcat tttttttaat agtgtttgat
     2401 tatttgattt aacttttggg ttatcataaa aatcctgaac tgtaagtgtt ccacctttaa
     2461 acgttgcaat cgtttcattg gacttgctac aacctgagaa tagtaaaatt gcacatgctg
     2521 aaaggcacat aattagtagt tgttttttca aaaaatttcc catcctttcc ttattaaaaa
     2581 cagtctaaca gaagaattag ggagacagtc tttcattttc gtaaggttgc tgtagatatt
     2641 cttagttcgt aaaaatctat tctctctatg atggagaaca tttacatagt tttcacgaga
     2701 caagaaagaa aagggttgtt aaacttaata taaaggaggg atgaaagatg atcaggatta
     2761 ttcggttgat tggatctgtt ttaatagtct tagttggttt tgttttagga attcttgtca
     2821 ataatgcact tgcggatatg tttattggaa atagtgaatg gattggagca attgccggta
     2881 cggtcggaca tttagctgtt tttttactag ctttatttct tgctagtaag gtcgaaggaa
     2941 aaagaataga agattatggt gtttctgttc gaggaaaaga ctgggggtat ttagcgggag
     3001 gtctcgttgt tggaattgga gtgtttctgc ttattactag tccattatat ttgacggggg
     3061 cgtatcggtt agattcagga aacgcaaaca ttgtgccgct gataacgagt tttattttat
     3121 ttattgcagt gggggcatca gaagagcttt tattccgtgg cttttttcag catcagatgc
     3181 ttagatttgg tccgttaata gcaatgattg gatctgctgc attatttgct cttttgcacg
     3241 gattgaatcc taacatgaca tttctggcag ttttcaatat ctttttagca ggttgctttt
     3301 tcagcgcact tatctacagt acaggaagtt tatttacggc tattggtgct catattactt
     3361 ggaattggac acaaggagct attttgggta taccagtcag cggaacgcaa gaacaaggtt
     3421 ttttctccac tctgatccaa tcgcaaaata caataattac tgggggaaac tttggggctg
     3481 aagcatctat atcaactaca ttattcttgt tagtcttgat tgttggtttg ttactctatg
     3541 catataaaac aaagcggatg aagaaataca ctgctgttta tcaataaaag atgaagaaag
     3601 caaggctgat atcggtcttg cttttttaag tctgtcattg attaaacgaa aaagtaaaca
     3661 aattcaagcg aatacacgac atcttttgtt agattttcac aactgactgt cgtgttctca
     3721 tcattttcat tttatataat tcctttatca aatagaaggt aattatatgg agagtgatag
     3781 aaatgaagaa aacagcgacc gtattatttg ttattcttgg gctgagtata attgcagcaa
     3841 tgattggaat cagtacttct gcaaatgaac gggcaaatga gaatctttca tttactgtga
     3901 aaactgatcg gattgtttat gacatgacac aacaagtcat tactattcca gtcaaaccaa
     3961 acaaaagcgt gaatgctagt gatgtacatg ctgttctaac atatggttgg gatggtaatg
     4021 gaagttcaga aaaagttatt ggggaagttt atttaaaaga cgtacaatgg accgctggta
     4081 ttgagtatac aataatgatt tctgcagaac tatcgattga tgagatcaag agtaaagata
     4141 aagttgattt gattgttttt tatgatggac agatgacgat cacggagaat ttgaaaccaa
     4201 gcagttggac agttgttgga ccgccaagca gtacggatag cacaagtagt gaaagcagta
     4261 cagaaaacac gagcggtgaa agcagtacag agagtacgag cagtgaaagt agcacagaaa
     4321 acacgagcag cgaaagcagt acggagagta cgagtagtga aagtagcaca ggaagcacga
     4381 gcagcgaaag cagtacggag agtacgagta gtgaaagtag cacaggaagc atgagtagtg
     4441 agagtagcac aggaagcacg agcagcgaaa gcagcacagg aaaagtaaat aatgaacttg
     4501 atttggtacc tatcatacca actaaggaac aaaagagaaa tgcagggagc aacctaaaaa
     4561 gtgtgctaaa acagcctgat tttgtttctc gtggaattga tcatcaagaa aaagcgaaag
     4621 aaacagataa tagaaaaagc gatttaccag aaactggtag tagaaccttg aacaagctaa
     4681 ttacatggat gggtgtgtta ctcattttga ttgtaggagc aagctatttt cgttctctct
     4741 tccatcgagt gaaataaaga ataaaagcaa cagatctaaa attgaataaa acgatagatt
     4801 tttgagtgaa tatttcattc gagagtgctg tattgcctgg cgaagttgta gctttgccag
     4861 atctattttt gtttactaac atatttgaga aacaggaaat aacaggaaca caaatggaaa
     4921 ataagagcaa gaataattga caatgcacac tgcgcatggt agtatctctg aaaaatcagt
     4981 aacgaatcta tttgtacgtt attcatgtag ctgtcggaat aagtctaaat aatagataaa
     5041 aaggagtatg tactttgttt gaagaattta ttgatatcaa tgaaaggcaa gtatatcaat
     5101 ttttgaacta ttgttatgaa agagacgaga aattatatgt ggtgaaggat atcgctttag
     5161 atctaaacta caccctggct aagatgaatt cggtgattca acaagcagaa tcgttttgcg
     5221 aaaggtatcc agaatataaa ttatcttttc tttcagaaaa caagatgata aaagtcgagt
     5281 tttcttctca gtttttactg agtaaagttt attctatact tttagaggga acgattggtt
     5341 atattttact ggattcactt tacaaaggta cttatcaatc cttagaaaat cttagtcaaa
     5401 aaattatata agtttgcgta cgattcaaag aaaattaaga gaacttaata ctattttgaa
     5461 aaactatcat ttatcttgca gcttgaaaaa aggtaatccg atagaaggag aagaataccg
     5521 tatccgatac ttttttcact tgatgtattg gcagtcattc gacgaaaatc attctgatat
     5581 attatcaagt ataaaaaata aacgtcaact attagaaaaa tttgcgaaac atgcgccata
     5641 tacgcgtagt atagatagaa agaaatggat cagcctacta agaatttcgt tgcaacgaat
     5701 gaaacaaggc cactcgatta cgtatgtccc agaagagatg aacaaatttg tgcatccatt
     5761 gataacaaaa gaagagttta cgcattacat actagagcct ttttttgaag caaacttctg
     5821 gtcgatgaat cacatcactc caacagagca agcttacctt tattttatgt ttggtgtaat
     5881 gaacacctat ttggaagaag atttgttgga tactccagat aaagtacaag aaagttgggg
     5941 ctggattgaa tcgtctattg tagaaaattt tgaaaaaaac ttcaagattt gttttacaac
     6001 ttcagaaaaa aattatctat atgtcaattt ggcaatgatc catttgtatt caagggtttt
     6061 tgggacaaaa aagaaggtgg atgcttttgg aaaatcagca gaagacagag attttcagca
     6121 tatatttcca gtgatgtatc caatcatgca acagtttttt agtggggtag ctcgcagaat
     6181 tccagagctt aatacttatt atgaaaagaa cagacggttg atttttcaat actgtatgtt
     6241 agtcagggat atttttataa aacacgaaca acctgtcata ttctatattc aatcaaagta
     6301 tggtaaaaca caggaattat gggcaaagaa aaggatatta tctatgactg aacgcccaat
     6361 aaaatatagt gcatccgctg atcagttacc tgatattgtc attagcgatt atcctatcga
     6421 caaaaaggca tatgatcaaa cgaatacaca gatattttac tggaatggac aacctacaaa
     6481 aaatgattgg gcgcgtctga aagctgaaat cataaaaatc aggaatgaga aaaatagatc
     6541 catattggaa aaattggatt tcgttaatta agaaaagcag attgtttatg ccaaaattaa
     6601 gacattgcaa aaagattatt ttctttaaaa tcgacaagat gaccagaaag caagagttcg
     6661 ttgagctctt gctttctttt agttttagaa aggaacatcc tgattttatc ttcgctaaat
     6721 ccagtcaaaa gttttgcaga ttggtcgtag ataatcggtt ttttcaataa agaaggggat
     6781 gtacgaatct caatatatag ttcactcaaa ctgagttcat ctattctgtg aacaagattt
     6841 ttataactaa tagaatgagt agagagcatt tcggtcacgc cgttgtttgt caattttagt
     6901 aagtggataa attcattttt ggttagtggt tgacgaatta agttctgttc gataaacggt
     6961 atatcatgga cgataagcca acgttttgcg cgtcttgaag acgagcagcc agaacctgta
     7021 tacaatttaa tcatcataca ttcctccaat agctaagtat ttatgaaata ataacatgaa
     7081 gaaatagtcg aaaagtgcca tttagaggtg taaaataaga aaatgttgtc acgtataatg
     7141 gaactagcta gataagaaat actattaaat caagaaaaaa gaaagaaggc acaagtatgt
     7201 caaacacagc cgacgcaata gtagtgatcg atttacaaaa aggggtatgt tttgattctt
     7261 cgccgataca cgaattagaa acagtgacag cattagtgaa taaaagaatc aaaacatacg
     7321 aaacgattgg gaaaccagtt attttcgttc aacacgaaga tgctcaatta gtgccacaaa
     7381 caaaaccatg ggagctattg tcggaattat ctattcctga gcacagttat tttgtgagaa
     7441 aaacccatgc gaattcattt tataagacta atttgagtgt tttattaact gaattggact
     7501 gtcattcgat cgaattatgt ggggcacaag cagagtattg tatagatacg actgtgaagt
     7561 ttgctcatgg attaggatac cagcttatga tgtgtagagg agcaacgaca acttatgata
     7621 atccttttat gaaagcaaag gaaacaatcg ctttttacga gaaaatttgg gataaacgat
     7681 ttctgacatt tttggagcaa taagttgttt cttcgtttaa aaaaagcaga atggatactg
     7741 gtttatccat tctgctttat tacgtaaaat gagaaaataa tcctgttcta tttttatggg
     7801 ataaaccatt tattttttgt ataaaaaatg tttgcgttta catataattc tcctagaaac
     7861 ataaaaagga gtgaatagct tgagaacaaa acaagtatta aagggagcgt tggtcatctt
     7921 atttgctgta agtacaagcg tcccgcaatg gacagtatat ggagagagca tttcttccga
     7981 atcggaatct cgggaggtag aaaccacaga aactactgat gctggaaatg aaacgaaatc
     8041 gagtacttca caaacagaaa caaatactga aacaacggtt gagacatctg ttactagcac
     8101 agatcaagaa acgaccgcta ccacagataa ccaacaaaca gaagaagcaa cagctacaaa
     8161 aatgtcaaac gacgaatcct cttctgctaa agtaagtgac aaagagaatg ggacagaaaa
     8221 tgatgatatc ggtaaaggtg aaaatttcaa tggtaaagaa tatttgaatt acttcggtaa
     8281 acagatgaca gaaatactta atggaccaac agaagacatc aagtcaaaat taatagagtt
     8341 attagtcact gagcttgctg ttaatgatta tggtgtagat aaagagatta tcaaacatct
     8401 gattgctact tatctattgt acgatatcgc ttttgcaaaa aacagtgtgt tgatgtcgat
     8461 gcttggaggt acagatgatg ggacaacgcc tacagaaaac acaggcttct atccaaatac
     8521 aaaacaactt catcaaactg tactaaacaa acaagatggc aaaatgatgg atgtatatgc
     8581 atattatgtc gatcaaggtt cagataaaac agtcatgatc catggcggat ttcgagggaa
     8641 ttggaataac gggatcgtta cagaagaata caatgatttt tataaggccg ggtataattt
     8701 attatttgtt gattccagag cgactggtaa tagcggaggc gattatgtga cctatggaca
     8761 atatgagtca gatgacgtcc tttactggat aaatcaagaa gttcgagagc ggccatctca
     8821 aaagatttta ctttatggtg gttcgatggg agcagctacg atgatgtctg tattagcgaa
     8881 agatattcct gtcaatgtca aaggaattat tgaaaattgc ggatttgcat caattgatga
     8941 acaactacgt tttacgtaca gccagacagt agcccccgca ctaccggatg ccataaaaaa
     9001 tcagttagac atcattgggg atcaagagca tgaagattta tttatgggcc ttttgaaaca
     9061 atactatttt gatcaggaga tgcatttgga tacaaaagct gctctaccta cgattggaat
     9121 gtcagattca ttgcctaaat tgattatcca tggaacagca gatgatgtag tacctgtgtc
     9181 taacgcgcaa aaattatatg agttatcagg agggtataaa gatcttttat tagtagaagg
     9241 agcagggcat ggaaaggcac aggaagttga ccatgctgcc tacacgaaac atgtaacgga
     9301 ctttttgaaa gtagtgttcc atgatcagat aaacgtaaag tatgtggatg aaaacaataa
     9361 aagtctactc aaagaccagg acgaaatcca gctttacgga gcttatggag aaaattatat
     9421 gacagagcaa aagacattcg aaggatacga attagcaaat gttgaaggac cgactgaagg
     9481 gatttttaat gagacgatac caactattat tttctattac aaaaaaatcc cagtagctcc
     9541 accaaaaaag caagatccga cagagaatgc agacaataag ggaaagaatg aacctcgaga
     9601 ggatcaatca agtgatgata cgcaaccgat tcagatgaca gtgaaagtac aaaaaaagac
     9661 agatgcaaaa aaaagaatgt tgccgaaaac tggagaaaag aaacattcgc ttctcattta
     9721 ttcaggtgga tttttatctg ttagcacgat tatcacatgg atttggcgaa gaaagagagg
     9781 gtaaatgaat gaaatggttc aagcgaatag tgattgcagt accagttatc atcgtagtta
     9841 ttttagtcac tgtatttttg attaatcaaa tcacaccaaa gccgattagc ttgcttgtta
     9901 gaaaacaatt tgacacaagt gaaaaagaac aagtatatgc ccgaccagat gatttccagc
     9961 aaaaaatcga acaaatcgat ataaaaaaag aaatatccta tccttcaaag tacaaaaata
    10021 ataatatgga tatctacaca ccgaaagtgg aaaagaaaaa attaccgatt cttttctgga
    10081 tgcatggagg cgcatatgta ggaggggata aaaatgattg tcgtgattat ttggaatatc
    10141 tatgttcaga cacgcagcaa atcatcgtaa atatagatta tgaacgatca ccggaagcaa
    10201 aacatcctac accagttatc caattgaacg aagccattca agcagctaaa aaagagataa
    10261 aagatcaagc agattggtcg aatatttcga ttggtggaaa ttcggcaggt gcacaaatta
    10321 gtggagaata tttgttagca cttcaaaatg aagaaattaa aaaagctgat catcttgatc
    10381 cgactttgga gaacaagcag attacgaagt ttatttcttt gtctggtctg ttggatcctt
    10441 ctgcacttac tcaagtaagt gataaaatct ctagctttct ttatgaaaaa tgtggatggg
    10501 cttactttga taataaggac tttgaacaat cagagaaggt caaaagttta gccttggtca
    10561 gacatgcgga tcgttggacg caaaacacat tcttaacgga tggaaataca aatacgttta
    10621 cgaaacaaat ggaagaagta acttctgtat ttgagaaagc gggggtgaaa gtcacaaaag
    10681 tagattatgc ggagaaaaat gctgttttga accatgaata tcagtttgat ttcagcaata
    10741 aacaagcgca agaaacatat caaaagttag taatgttttt caaggagtaa agtcttatga
    10801 tgcaaaagag gttgtaatct gtcacttagc tccaagtcac aaggagcaat ttcaataaac
    10861 aagcttttca tggtttatga atattgtaac ttgtgactga ggagttagag atggcaaggg
    10921 gcacctttta ttcgaaattt gggaatatca cacgattatt gtcacgatct cttttacgta
    10981 aattttataa gtcagatttg ttgttttgat cgtttaaaaa aagcaacaaa tccgaagcct
    11041 gcggaggcaa gaagggacaa aaagccgagt ggtaagcaaa agaatgctgg ttccactaat
    11101 attccgttgt tattgatata aggctgggaa caaagatgaa tagcaaataa aatcaatgca
    11161 ctagcgaaaa aaagcaaaga aatatggata gctttcataa gcagcacctc cttttttata
    11221 attgtatgat aaatagatta agaatctata aatgtgacaa gtttgttagg atgtgaataa
    11281 aatgatcatc tagcgaactt gaaacaaaga tggctaatga taagcaatta atgattcagc
    11341 aatcagatag gtacaaagtt taccatcata cgtctagacc aataatagaa aatatgggaa
    11401 tataatagaa aagatttagg atacccccaa aagaatttcc ccaaaaattc gatgtatcct
    11461 aaactttttt attttacata acaatcatat caactgtgaa ataaaattac aaatgataac
    11521 ctaaaatgag gccaaaagcc agtgttatca aggaaatact taattcacca aaattttttt
    11581 taatttttta gaaaataaga aacaaaaata ataaaaaaaa gaaaagataa attttttact
    11641 actgtatact aactttgtta tcgcatctaa caagtaactc gaataatacc aatacaacct
    11701 tctccaaaga tgcgatgaca tacgttctaa cccaccgact attgatgttc tgtaccaccc
    11761 ttgcagaacc aaaaaagcca ctaccaaata tttggtagtg gctttttttg ttttatggat
    11821 ttttcgcttt ttctgttatg gtgctattct ttgatttcag aaatagtatg tcgaacttta
    11881 gcgatagaac gacgtgattc tttttcgttg taaaacatca aaatcacata tagagagtcc
    11941 aaaatgctta gttgagaaat acgggaagct aaagcttcag atcggaattc tgtttcttca
    12001 gaaatagaaa taaaaacgac atcgccaagt ttagcaaggg aagaattagc ttggctagta
    12061 ataacgattg tttttgctcc agtttttctt acggtttcag caatatggat tgtttctttt
    12121 gattgccctg tgtgagaaat caaaacagca cagtcttttt ccgtcaataa agaagcttcc
    12181 atcaattgaa tatggtaatc cgtgctgtga gaagttgcga taggggaacg caaaaattta
    12241 tgataagcgt cagttgctac aatctctgaa ccgccaacac cgaaaaaata gacacgttta
    12301 gattcattga taatagtagc cgcttttttt agatcctttt gttgtaacaa cttttttgtg
    12361 tcatttaaag ttgaaatact agactcgaac accttttgcg ccatagtgag ctcagtatct
    12421 gttttctgaa tattttcgtg gatagaaata gcagatagat cattttcttg gatcaacatg
    12481 gccattttaa aatctttaaa tccattataa ccaagttttt ttgtaaattg aaaaaaagta
    12541 gagtcagcaa tgcccagttc acttgctaaa ttgctaatag gactatgact tactttgctt
    12601 ggattttcta aaatataatc ggcgatagcc tgttcttttg ggctgaagtc gttgtacatt
    12661 gttttgattt gtagtcttat ggagacgctc atgagaattc ctcattttca taataagatt
    12721 atcatttttt ccgttctcat taatgttaaa gatacaagga gagtaggaaa aaatcaaaca
    12781 aaaaatattt ttgtaaaatc tatttacaaa aaaatttttt tgtataatga gggtataaga
    12841 aatggaggcg ctatcatgga aattggtgta attggtttgg gaaaaatggg attgaattta
    12901 gctttaaact taaaagagca ccatcacaaa gtacaaggac tcgacatttc aagtacagct
    12961 gttagtcagg caagagaaaa tgacattcct gcttatcaag agatctctga atttctagct
    13021 acttttgagc agaaaaaaat catctggtta atgcttcctg ctggagagat aaccgaaaac
    13081 atgttaagaa aactttttcc aaaactcgga ccaggagaca ttattattga aggcggaaac
    13141 tcttattata aagattcaat ccgtcgagca gaagagtttc agaaaaatga aatcggttac
    13201 ttggattgtg ggacttctgg aggaatcgaa ggagcacgac atcaagcctg cttaatgatt
    13261 ggaggagata aagaaacttt tctatccgtg gaacaattat tcaaagatgt agcaattgaa
    13321 agaggatatc tgtatgctgg tgcatccgga agcggacact tcttgaaaat gatccataat
    13381 ggaatcgaat atggaatgat gcaagcaatc ggtgaaggat tccaacttgt acaaaaaagt
    13441 gagtatgctt ttgacttgga acaagtcgca cgtgtttgga atcatggttc ggtgattcgc
    13501 ggctggctga tggaaatcat ggaagatcag ttaaacgaac atcccaattt aagtaattat
    13561 cggggaatcg tttctgcatc aggtgaagca aaatggacgg ttgaaacagc gctagaaatg
    13621 gctgttccag ttccagtaat tgcaactagt ttgttcatga ggaatctttc acaggaagaa
    13681 gatagttttt cagcaaaagt agtttcagca ctaagaaatg ggtttggcgg acatgaaata
    13741 gttgaaaagg gtgagtaaaa tgccagatta tcatatagca atcgttaatt ccagcagttt
    13801 cggaagagtc tttccacacc acttggaaag attaaagtca gttggagacg ttcatttctt
    13861 tactgttgat caagagattc caggaaaaga actagcggag ttacttaaag gattcaatat
    13921 cattattgca agtgttacgc cattttttcg acgagatttt tttgaaaaca aagatgaact
    13981 gctattgatt tcccgacatg gtattggtta caacaatatt gatttaaaag cagcaaaaga
    14041 acaccagaca atcgtatcta tcatacctgc tttagttgaa cgaaatgctg tagcagaaaa
    14101 taatataact aatttgttgt ccatgcttcg acgcacgaca caagcacaag caagagtcaa
    14161 ggacgatcga tgggaagatc gggcggagtt cgtcggtagg acgctgttta acaaaacagt
    14221 tggtgtgatt ggtgcaggaa ataccggaag cggagtaatc gaaattttgc gtaacggttt
    14281 tcgctgtgat gttctagctt atgatcctta caaatcagat ttatacatcg aaagctttgg
    14341 tgcaaaaaaa gtagaacttc aagaattact ggaacaatca gatatcattt gtttatgtgc
    14401 gaatttgaca gaagagaact atcacatgat ttctgataaa gaagtcgatc agatgaaaga
    14461 aaatgtttat ctatctaata gtgcaagagg cgctctagtt gatgaagaag cgatgattaa
    14521 tggtctgaaa agcggaaaga tcgcaggata tgccacagat gttttagaag aagaaccggg
    14581 acgtcaagat catccgtatt tagcgtttga acaagtagtg attacgccac atatatcggc
    14641 ctacacaagg gaatgtttag aagcaatggg tgaaaaatgt gtcagagatg tcgaacagat
    14701 cagtcagcac cttcttcctg aacgttctgt ccagacaaag agcaactata taaaaggatg
    14761 atgaagaaaa tggtgaacgg tttaaaagtg aaaacaggcc ctcaatttta tttatatgaa
    14821 gaaggtggta tttcaaaagt ttctgatctc ttgaaatcat acggcgcgaa aagagtactg
    14881 gtcacccacg gaacggtatc atgggaaaag gcgctaccta aattagtatt tttgaatgac
    14941 gaaacgattc aattttttta tcatcgttat tctggagaat gtagttatgc tgaagccaga
    15001 cgaatagcta caatcatcaa aaaaatgaaa tcgatttcgt agttggcgtt ggcggaggaa
    15061 aactgaccga tctagtgggt tatagtgcca atcttgcaag aatcgatttc ggagtcatcc
    15121 ctactttagc tagtaattgt gctccatgga cacctcttgc tgtcatgtat aaggagaatg
    15181 gacaagcaga agggaaaaca gaacactttt acagacaagc tgcttttttt ataacagatc
    15241 cccaattagt gattgattcc cctgttccat atttcattgc cgggttagca gatacactag
    15301 caaaatggta tgaatctgag ctcatcttag aacaagattt tttgcaagat gaatcatttt
    15361 tgaagctagc acaagatact gcgaagatct gcaaagaaga gattttgaat cattctgcta
    15421 aagcaatcca agatatggca caaagaaaac tgacatcgga gttcaaacag ctttcagaga
    15481 ttatctttgc tgttgctggt ttagttggag gattaggaga taaatacgca agaaatgtgg
    15541 cagcacatgc tatgcatgac gctatcagta aatatattcc ggaaagccat cgttttctac
    15601 atggagaaaa agtagcgtat gggattttct atcaactggc actagaaggt cgctgggaaa
    15661 aaatagacga gttatatcct ttgtatcggg aattagaact gccggtttct ctagaacaaa
    15721 tgggattatt tccgaaagat aaacagataa tagataaaat ggctgaattt attgattcga
    15781 aagagaaagt gcatttgatt cctatagagg taacacagaa atcactgata cagtcaatga
    15841 tggaactgga aggttatatc agaaagatct gaaggaggag gacacatgga aacattaagt
    15901 gtagtacaaa gtttgttgat tgctttatgg gtagccgcgg ttatgtcccg ctggttaggt
    15961 ggaggagcca cgttgacctt acgtttttct cctttgatga ctggattagt cgtcgggttg
    16021 atcatggggg aagttgcaca agcgatgatt gttacagccg ctttgcaaat gatttatatg
    16081 ggggtttttt ctccaggagg atcaatgcca gctgaaccat ctattgcagc agctatcgca
    16141 gtaccagtcg cattattagg ggatctgtcg ccagaagcag cgattgccgt tgcggtgcca
    16201 gtcggattgc tcggaagtta cttgtatcag tttcgttttt tcatcaatac ttttttaggt
    16261 aaatatacag acaaagctgt agaagatttg aatacaaaag ctatggcccg atcaattatt
    16321 ctttatccaa cggttgcctc atttttatta ttcgttccgc ttgttttcat tgctttatat
    16381 ttaggggcgc cattgatcgc tgatgtaatc aaagctttag agggaacagt cgttatccac
    16441 attcttgaag tagtaggtgg agggttagcg gcaatcggta tcgcgacaac tgtatacgtt
    16501 atcggtcgaa aagattatct ggtctttttc ttccttgctt attttatgag tgttgttttg
    16561 aaatctttag atattacgat ggttacttat gccatttttg ggattattat cgctttgatc
    16621 tttgtccagt cgcaaaaagg gaaacaagta cagcctgcca caagcaataa caattcagca
    16681 tcttttgatg acgaagatga ctatgatgac ggattttaat ttaggaggaa tgggaaatgg
    16741 ctgaaataat ggataatcaa acaggtgctc ctgaagaaat cacgaaaaaa gatgtaacaa
    16801 aagcctatct gagatggcat tttgcaaacg aaattcctca ctcttttgaa cgttacttgg
    16861 cgccatcact actatacgga atgatgccaa tcttgaaaaa actttataaa gatgaagact
    16921 ctttaaaagc agcatataaa cggcagttac tgttcttcaa cactcaatta agctggggtg
    16981 gcggtgtgat tactggccta atgtcttcta tggaacagca aagagcagaa gaagagtatg
    17041 aagaaaaaga gatcttgatg caggatgact tgatgtataa cacgaaagcg ggattgatgg
    17101 gggcactagc aggaatcgga gatgcaatcg attcaggaac cgtccaatat atttttatcg
    17161 caatcgcggt accttgggca caacagggaa gtcctttagg cgcacttttt ccttttatct
    17221 gctttgggtt gtatcaggtt ttactagggg tatttttcgc aagacaagct ttcactatgg
    17281 gccgtaatgc tacaggccta aagcaaaaca ctggtgttca gacagcgatt gaaatgctat
    17341 cagttttagg gttgtttatg atgggggtgc ttgcgggaaa ctatgtaaaa gtagaatctt
    17401 cattgaaatt tgcgatatcc ggacgtgagt ttgttatcca ggaaattctt gatcaaatcg
    17461 tgccagggat attaccttta gctgtcgtaa tgggtgtgta cttgttctat acgaaaaaag
    17521 gattgaaagt cacacaagca ttattatggc tgactggaat attaattgtg ctagcaggaa
    17581 tcggtatttt ataaaaagaa gggaacgtga ataagatggg tgtagtcaat ttagcgcgtg
    17641 tggatgagcg tttgatccat gggcaagtaa tggtaacgtt atctcaaaaa agtggagtga
    17701 attccatttt tgttgtagac gaagttgtag caaaagataa atttatgaga gatctttaca
    17761 aaaatgcagg aagccgtaca ggacaaaaaa caattgtcat tacaccagag aaagctaaat
    17821 tctattggga tgaatatcaa tttaaagaat acaattgtat tttgatcgca aaaacagtcc
    17881 aaacgattta cgacttagta aaacacggaa tccccatgga agaattgaat attggaggga
    17941 ttgctcaaaa aaatcctgat acagatcttt tagtgacgaa atctgtttat ttaaataaat
    18001 cagatgctga aaaattgaaa gaaatgaatg aacaatatgg cgtaaagaat atttattttc
    18061 aagccactcc atctgcacct aaaacaggat taaaagatgt attgaacaag tttgatttgt
    18121 cataaacaaa tgcttagaaa aaagataaag agggcgaaaa aatgaagaag aacgagctgt
    18181 ttcgtgattg ggaatttcgt tatcgctata tttaccgtaa gcgccgtaca aaaaagagta
    18241 aacagcgttt tctttcagca ttagtatcag atatttacag tatgagaaca gatgtgacag
    18301 tcattgctta cgatacgcta gcctaccgat caaaaaatat ttatgttgga gatatcgaaa
    18361 aagcagagaa agttatctgt acttattacg atacacccgt tcatgcatta ggaagttatt
    18421 tcatgtttga ttggaaagac caaaggaaaa aaacaatcta ctctatatta ttgtccttca
    18481 ttttgttgtt cagtttaggt tggtggggaa tgatgatata taacaaaaat cctcatcacg
    18541 tattcgatct gctatccgta taaacaggta tcactgtact agcatttggg agctactttt
    18601 tcttattggg aaaagcagct cgcggatggc ctagcagaca aacttttatt cgaaatactt
    18661 catccatatt aacgatgcta gaaatgatcc gtacgattga tgatccgagt gtagcgtatg
    18721 cgtttgttga tgaaggctgt tatggagaaa agggcttaga cagcgtgaga tcgggtatga
    18781 aaaaagaggc tattcttttt tatctagact ctgttggggc agatacaccg ctacagtttt
    18841 cgggaaatta tttttcaaat aaagaacagt ggctgaaaca agttgataag ttgaaagaaa
    18901 aaaacgtcaa ttatattttt agtgcaagaa aaaagcaagc gcagttcttc tatttgacca
    18961 aaacagattt acgagggaaa acatttaatt ggcaaaatgc caatcagatt attgctcttt
    19021 ttcgataagt aaaaagtgca aaacaaggag tgagaagact aatgttggaa atcatgatcg
    19081 ttacacatgg taagttgagc gaaggactga aagatgcggc ggaagtaatt gtcgggacat
    19141 cgagccatat tgaaacaatg tgcttagagt ctggggagga catccaaaca ttcggagaga
    19201 aggtcaaaga aaaattaact atgatcaaca cagcagaacc catgctagta tttgttgatc
    19261 taattagcgc aagtccgtac aatcaagcgc tcttggcagt caatgaactg actgaagaaa
    19321 agaaagcagc cacttatgtc atagggggcg tgaatctgcc aatgttgtta gaagcaatca
    19381 atcatcagat gcttcacaca ccgatagaag aagcggtgga acaaattgtc aaacaaggaa
    19441 aagataattt aactgtttgg catgcttcag cggaagaaaa aaaattagat gaggacgatt
    19501 tttagaagga aggctgaaaa gatgaagtgg ggatttagat ggtatggtga aaaaggggat
    19561 tcgatcccat taaaatatgt tcgccaaata ccgggaataa atgggataat tggaactttg
    19621 ctgactaaaa tgcctggaga tgtttgggag atttcagaaa ttgaggagtt gaaagcatct
    19681 gttgagagcc aaggactgga actgcttgga attgaaagtg tagcgatcca cgatgcaatc
    19741 aaggcaggga cagatgaacg agattactat attgataact atatacgaac aatcaaaaac
    19801 ttgtctcgat gtggggttcg attgatttgt tatagcttca aaccaatttt tggatgggcg
    19861 aaaactgatt tgtttaatga aaacaacgat ggaagctatt ctcttttata cgatcagaaa
    19921 atcgtcgatc agatggagcc aggagaaatg tatcaattga tacacagcca gtcaaaggga
    19981 ttcaaattgc cgggttggga agaagaacgg ttgaaaaaat tcgctcagct ggaaaaactg
    20041 tatgaaggaa tgaccgaaga agaattattt gaaaatctga cttatttttt agaaaaagtg
    20101 attccagtgt gcgaaaaagc agatgtaaaa atggctatcc atccagatga tccgccatgg
    20161 gaactttttg gttttcctag agtgacgaaa aatcttggcg acttgaagaa aattctaaat
    20221 gcagtagatt ctccatataa tggactaacg ctgtgcacag gatctttagg ggcaaatccg
    20281 gaaaacgatt tagttgaaat catccatgag gtaggttcgc gcatccattt tgtccacttc
    20341 agaaacgtgg agtttttagg agaaagaaag ttcaaagaaa cggcacatcc ctctacagaa
    20401 ggctcccttg atatgtatca tattatgaaa gcgttggtgc aagaagggtt cgacggtcct
    20461 atacgccctg atcatggtag aaccgtgtgg ggtgaagtgg ctatgcctgg atatggcctg
    20521 tatgatcgtg caatgggact cacttacatg caaggtctat atgaggcgat aagtaaggaa
    20581 aaaccactgg gagagcatta tgaataaaat caagctcttc atctttgata tggatggcct
    20641 tttattagaa actggacgtt tagcttatcg tgcttatgta aaatcagcac aaaagtatga
    20701 ttatgaaatg cgtaaggaag tctattattt actaactggc caaacagaaa tggcgataag
    20761 agaacaaatg ggcattttgt acggagaaga tgttccatat atcaaatggc gagaagcaat
    20821 caatcaatat aaagaaaaaa tcgtgaaaga agataagaga gtatatacga aaaaaggagc
    20881 agaagaaatt ttatcttttg caaaagaaag aggaatccat acaatcgtgg cttcgtctaa
    20941 tacacgggta aaagtagaaa tgtatttaaa aatggaaaat ctatatgcct tgtttgatca
    21001 aatcatttca ggggatgatg taaaaaaagg aaagccggaa ccagagatct ttttgaaagc
    21061 ttgttcaaaa atgaatattc ctccaagtga agcacttgta tttgaagact cgattgcagg
    21121 gatcgaagca gcaaggcgag cgggcatttt gtcttttctc atcaaagatc atttgatagg
    21181 gttgcccgat cataatggga agcataagtt gaaagtagat gtaagtttat atgaatccaa
    21241 taaagcagac tatgaatttt atgacctgca tcaagcaaaa cgatttttag aaagtaaagg
    21301 gttgtatctc taataaagag gactattttc tagtcctctt tatttattgt cctgcacgac
    21361 tgagggaaca gatgaaaaag aagtaatttt ttattttgaa gtatctacct tatttgtagt
    21421 gaacaattta gaacaaacac aaaacgcatc tactgtt
//
LOCUS       DACGLE010000043        20362 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0534
            SAMEA3304449-rid9560453.denovo.046, whole genome shotgun sequence.
ACCESSION   DACGLE010000043 DACGLE010000000
VERSION     DACGLE010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304449
            Sequence Read Archive: ERR985064
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20362)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20362)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702225.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702225.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 20:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,045
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 27 of 67
            Pseudo Genes (incomplete)         :: 25 of 67
            Pseudo Genes (internal stop)      :: 19 of 67
            Pseudo Genes (multiple problems)  :: 4 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20362
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304449-rid9560453.denovo.046"
                     /strain="VREN0534"
                     /db_xref="taxon:1352"
     gene            292..1431
                     /locus_tag="IXT20_001742"
     CDS             292..1431
                     /locus_tag="IXT20_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgE/DapE family deacylase"
                     /protein_id="HAQ4860183.1"
                     /translation="MKKEEKIAILQEIIRIKSVNGNEGEVAAYLNKLLARHDITGEIV
                     SYRDGRDNLIARYQKGQSGKVLGLSGHMDVVAAGDESSWTYAPFAAEIHGNRLYGRGA
                     TDMKSGLAAMVIAMIELKESGKPFNGTVKLLATVGEEVGELGGEQLTKAGYVDDLDAL
                     IIGEPTNYSLMYTHMGSINYTVTSHGKEAHSSMPDQGYNAINHLNEFITKANAEMNHL
                     AETIENPVLGKTIHNVTLISGGNQVNSIPSHAQLQGNIRSIPEYPNDKIIALLQSIVN
                     ELNQETDYHLELMIDYNKIPVKADPDSPLIHSIQQQFSQPLPLVGAAATTDAAEFTKA
                     NHSFDFVVFGPGVVTLPHQVDEYVEIDNYLDMIEKYQGIILSYLA"
     gene            complement(1498..2073)
                     /locus_tag="IXT20_001743"
     CDS             complement(1498..2073)
                     /locus_tag="IXT20_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAQ4860184.1"
                     /translation="MNKNKMATRVLTVMALCIGINYIGGTIALWLRLPIYLDSIGTIF
                     AGALLGPVPGMLTGLSSGILSGVTTDIFSLYYSPIQILTGLLAGLILPRYLGKDKNSL
                     KLLLLALILSVPGTIVSSIITVQLFGGITSSGSSMIVQLLHGLGLSQTTSVILVQVGT
                     DYLDRLLSIVVVSLVITMLPQRLIYSKRKIG"
     gene            complement(2078..3052)
                     /locus_tag="IXT20_001744"
     CDS             complement(2078..3052)
                     /locus_tag="IXT20_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HAQ4860185.1"
                     /translation="MRKVIIDCDPGIDDTLALSLAVKSPDIEVVAITVVCGNVPVDIG
                     TQNVLKCLERCDRLDIPVYQGMEKPLKQPFISAQDTHGLDGLGDTNFPMVLAKQAESL
                     HAVDFLTDYFKEKTDTSVIALGPLTNIASALKNSPSVGKHMDRFVSMGGTYKSHGNCS
                     PVAEYNYWCDPEAASYVFEKLKQPIEMVGLDVTREIVLTPTILEYCCQMNPEEGEYLK
                     AITRFYFDFHWKQERILGCVINDPLAVAYFIDESICEGFKSFTAVETQGISRGQTLVD
                     RYEFWQKPANSKIMTKVDTRLFFRKFLTVLLNAQEETIMKDLERLKMG"
     regulatory      complement(3076..3120)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            3335..4303
                     /locus_tag="IXT20_001745"
     CDS             3335..4303
                     /locus_tag="IXT20_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAQ4860186.1"
                     /translation="MVIFYFILILIFIGISYHFLSYFYAKKLVRAGVQRGYDWYEETG
                     HRLMDPAAVTSVEKKRQKLEEECESFWEQGIPKTIKSYDGLKLAGQMFLQPTQQNKWV
                     ICVHDYRSTGKRDMSHIGKRYAEKGFNVLIPDLRAHGESEGEIIGMGWLDRLDLIAWI
                     QLILDEQPDASIILHGSSMGASTIMMASGEKLPSAVKGFILDSGYVSVYAEFRYMLSK
                     ITVFPKKMIMRYANHYAQKYAGYSLKQASATRQLGSNHLPLLVIHGERDHFVPTEAAY
                     TIQNATAGDKALLLVPEAEHLEASMKDPKTYWTVIFSFIEQRITMD"
     gene            4416..4694
                     /locus_tag="IXT20_001746"
     CDS             4416..4694
                     /locus_tag="IXT20_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HAQ4860187.1"
                     /translation="MEIKEEKNRLALFNDEQQEIGEMTWSDAGPDIMIIDHTFVDPAY
                     RGQKLAEKLVYTGVELARREGKKIIPSCPYAKKEFEKKPEYHDVLRQS"
     gene            4812..5048
                     /gene="secG"
                     /locus_tag="IXT20_001747"
     CDS             4812..5048
                     /gene="secG"
                     /locus_tag="IXT20_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HAQ4860188.1"
                     /translation="MYNLILGIVIVLSIVMVIAIMMQPSKQNSAASAFTGGADQLFGK
                     QKARGFEAVMQRSTAVMGAVWMILLFVLAFLSSK"
     gene            5160..5906
                     /locus_tag="IXT20_001748"
     CDS             5160..5906
                     /locus_tag="IXT20_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HAQ4860189.1"
                     /translation="MKQLPKPLYAKHGKRAVLLLHAYSGSPNDVRMLARFLEKADYTV
                     YAPLFKGHGTMEPYDILQEKAESWWADTKKAIHFLQSEQFSDIAVLGLSMGGIFAVRA
                     LEEESVIGGGFFCSPLSPVETNVPENFEKYVRQVLKIAGKSEKEINEKAVSYRSLAEH
                     QLMDIQDQAAIAESRLSDIQQPIFLAQAGKDEMIDPNGVFETARKLSRQRVTLQWYPE
                     SGHVITVGTARRELEKDVLEFLEKLPWNEE"
     gene            5909..8266
                     /gene="rnr"
                     /locus_tag="IXT20_001749"
     CDS             5909..8266
                     /gene="rnr"
                     /locus_tag="IXT20_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HAQ4860190.1"
                     /translation="MTKETIKEKILFFMESSRKRSFSMEEIAEGLGFQKSDDFKLLVQ
                     TIAQMEREQSIVFNKKGKVKLPLKPILVEGTFRANERGFGFVTIDPEEDDIYIPKEAT
                     GFAMDGDTVAIDIMKRANTDMDRGAEGKVVEIRDRATTQLAGEFVAYSDEEIQETDLY
                     GVVIPKDRKLNQFKVYAAAEGVRPVDGSIVLIELTHYPEKGYATSLEGIIKQVIGHKN
                     DPGMDILSIVVANGIPTKFPDDVLSEADEVPDQISEKDIVGRRDLRDQLIVTIDGEDA
                     KDLDDAVTVKKLENGNYFLGVHIADVSYYVTQGSQLDREAYERGTSVYLTDRVIPMIP
                     QRLSNGICSLNPHVPRLTMSCEMEIDPNGQVVSHEIFPSVIQTTERMTYTAVNQILED
                     QDEETMQRYAQLVPMFQEMGELHRILEMMRIKRGAISFEDREAKILVDAEGHPNDIQL
                     RSRGIGERLIESFMLAANETVAEHYNKLNLPFIYRIHEQPKEEKMQRFFDFASALGIL
                     VRGTKGTITPKDLQHVIEGVEDKPEAAVINTMLLRSMQQARYSEDNFGHYGLAADYYT
                     HFTSPIRRYPDLIVHRLIRSYSEDKSESNQSSWAAELPEIADHSSKMERRAVETEREV
                     DSMKKAEFMMDKVGEEFDGIISSVVKFGLFIELPNTVEGLIHINELKQDYFHFVENHL
                     ALVGERTGLTFKIGQKVRVKVVKADPDERAVDFELVSAEEVTALERPKARNGKSGKRE
                     GKQAGKRSEKNGKKNKRYSDSKQTFGKKKGKKPFYKGVKKKKKKK"
     gene            8288..8752
                     /gene="smpB"
                     /locus_tag="IXT20_001750"
     CDS             8288..8752
                     /gene="smpB"
                     /locus_tag="IXT20_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HAQ4860191.1"
                     /translation="MPKGEGKLIAQNKKARHDYSIIDTMEAGMVLQGTEIKSIRNSRI
                     NLKDGFIRVRNGEAFLHNVHISPYEQGNIFNHDPLRTRKLLLHKKQIVRLENELKNTG
                     ITVVPLKVYIRNGYAKVLIGLAKGKKSYDKREDLKRKDIDRQIDRTLKNFSR"
     gene            9054..9773
                     /gene="atpB"
                     /locus_tag="IXT20_001751"
     CDS             9054..9773
                     /gene="atpB"
                     /locus_tag="IXT20_001751"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HAQ4860192.1"
                     /translation="MDERSLTFHIGPIWFDGTVCIMVLLTCLIVFFLAYFFTRNLKLK
                     PTGKQNAIEWVIDFSRGIVTDNLPRKELSNFHLLAFTLFLFVFVANNIGLVTKIVLPN
                     ETTLWKSPTADPFVTLTLAFIMILLTHLFGVKKLGFKGYFVNSFLKPYSFMFPMKLIE
                     EFTNLLTLALRLYGNIYAGEVLLTLIAKMMTSLGWFSLPLAIPLEMVWIAFSLFIGSI
                     QAFVFVTLSMVYMSHKIEVEE"
     gene            9826..10041
                     /gene="atpE"
                     /locus_tag="IXT20_001752"
     CDS             9826..10041
                     /gene="atpE"
                     /locus_tag="IXT20_001752"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HAQ4860193.1"
                     /translation="MNYIAAAIAIMGAAIGAGYGNGQVISKTIESMARQPEMSGQFRT
                     TMFIGVALVEAVPILGVVIALILVFAV"
     gene            10132..10656
                     /gene="atpF"
                     /locus_tag="IXT20_001753"
     CDS             10132..10656
                     /gene="atpF"
                     /locus_tag="IXT20_001753"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HAQ4860194.1"
                     /translation="MLNQLAIAEVGNAMLGNIIVVSGSFLILLALLKHFAWGPISDIL
                     KKREDKIANDLDSAEQSRINSAKMEQEREQQLLASRSDAADIIKNAKESGELSRQNIL
                     KDAQEEAARLKSKAQADITVERDSALNSVKDDVAELSLQIAEKILNKELSPEMHESLI
                     NQYIEGLGSSNETR"
     gene            10643..11185
                     /locus_tag="IXT20_001754"
     CDS             10643..11185
                     /locus_tag="IXT20_001754"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HAQ4860195.1"
                     /translation="MKLDKYTVGRRYGKALFELAIDSNQAEEIYQDLVSLRQIYEQVP
                     GLGNMLSDVRLEPHEKREIMDKLVSGYDGIVKNFLEVVYEYNRMDDLLFMIEEYERRY
                     DEHQGLLLGSVTTAVPLSDEQRIQLEKNVAKTMDYQSVELKQIVDSSIIGGAIVEANH
                     RVIDGSIRTQLEKMRNQLNR"
     gene            11208..12764
                     /locus_tag="IXT20_001755"
     CDS             11208..12764
                     /locus_tag="IXT20_001755"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HAQ4860196.1"
                     /translation="MAIKAEEISALIKEQIENYQNVLSVEEIGTVTYVGDGIARAHGL
                     ENAMSGELLEFSNGSYGMAQNLESNDVGIIILGDFESIREGDKVKRTGKIMEVPVGEA
                     LIGRVVNPLGQPIDGLGEIVTDKARPVEAMAPGVMQRKSVNEPMQTGLKAIDALVPIG
                     RGQRELVIGDRKTGKTSIAIDTIINQKGQDMICIYVAIGQKDSTVRTQVETLKKYGAM
                     DYTIVVNAGASQPAPLLYIAPYAGTAMGEEFMYNGKHVLIIFDDLSKQAVAYRELSLL
                     LRRPPGREAYPGDVFYLHSRLLERAAKLSDDLGGGSMTALPFVETQAGDISAYIPTNV
                     ISITDGQIFLESDLFYAGTRPAVDAGLSVSRVGGSAQIKAMKKVAGTLRLDLASYREL
                     EAFTQFGSDLDAATQAKLNRGRRTVEILKQKLHAPLAVEKQVVILYALTHGFLDSIPV
                     DSILDFEHELFEYLDTNHSDIFETIRTTKDLPEEETLNSAIQEYKDMFLATKGNNSST
                     EDKLKSIQNA"
     gene            12778..13680
                     /locus_tag="IXT20_001756"
     CDS             12778..13680
                     /locus_tag="IXT20_001756"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HAQ4860197.1"
                     /translation="MGASLNEIKTRIASTKKTSQITRAMQMVSASKLTKSEASSQKFQ
                     VYATKVREIVTHLTATQLNDIASENPRGDINYNSMLLSRPVKKTGYIVITADGGLVGG
                     YNSSILKQTMSILEEDHQSPDDYVMIAIGGTGADFFKARGINLAYELRNLSDQPSFDE
                     VRKIVNMATTMYQNEVFDELYVCYNHHINSLTSQFRVEKMLPISDLDPEEATTFEQEY
                     IFEPSKEEILSQLLPQYAESLIYGAIVDAKTAEHAAGMTAMKTATDNAANIIDELTVS
                     YNRARQGAITQEITEIVAGASALE"
     gene            13703..15109
                     /gene="atpD"
                     /locus_tag="IXT20_001757"
     CDS             13703..15109
                     /gene="atpD"
                     /locus_tag="IXT20_001757"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HAQ4860198.1"
                     /translation="MSSGKIVQVIGPVVDVEFSLDQSLPDINNALVVYKNDENKSKVV
                     LEAALELGDGVIRTIAMESTDGLQRGMEVIDTGKAISVPVGKETLGRVFNVLGDTIDL
                     ETPFPEDAERSEIHKKAPAFDELSTSTEILETGIKVIDLLAPYLKGGKVGLFGGAGVG
                     KTVLIQELIHNIAQEHGGISVFTGVGERTREGNDLYYEMKDSGVIEKTAMVFGQMNEP
                     PGARMRVALTGLTIAEYFRDVEGQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQP
                     TLATEMGQLQERITSTKKGSITSIQAIYVPADDYTDPAPATAFAHLDATTNLERKLTE
                     QGIYPAVDPLASSSSALAPEIVGEEHYKVATEVQHVLQRYRELQDIIAILGMDELSDQ
                     EKVLVSRARRVQFFLSQNFNVAEQFTGLPGSYVPVEETVKGFREILEGKYDDLPEEAF
                     RSVGRIEDVVEKAKTLGY"
     gene            15123..15545
                     /locus_tag="IXT20_001758"
     CDS             15123..15545
                     /locus_tag="IXT20_001758"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HAQ4860199.1"
                     /translation="MDQYLTVNVVTPAGLVYDHHAAIVVARTTAGELGILPKHAPIIV
                     PLTIDEVRVKRTDSDTHVDWIAVNGGIMEVRDNVVSIVADSAERERDIDVSRAERAKQ
                     RAERQIEEAKAKQDTNELKRATVALHRAINRIKVSKHS"
     gene            16014..16244
                     /locus_tag="IXT20_001759"
     CDS             16014..16244
                     /locus_tag="IXT20_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1146 domain-containing protein"
                     /protein_id="HAQ4860200.1"
                     /translation="MQVYGIDAVLRIVSHLAFIYLAFWSLRSLRIETFFRPMHNTQIR
                     MAIVLFSIFLGYTASNFFLEVIALCRNLFVSL"
     gene            16341..17642
                     /gene="murA"
                     /locus_tag="IXT20_001760"
     CDS             16341..17642
                     /gene="murA"
                     /locus_tag="IXT20_001760"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HAQ4860201.1"
                     /translation="MEEIIVRGGNQLNGTVRIEGAKNAVLPILAASLLAEEGITTLDN
                     VPILSDVFTMNQVIRHLNVDVDFDEQKNQVTIDASRQLEIEAPYEYVSQMRASIVVMG
                     PLLARNGHAKVAMPGGCAIGKRPIDLHLKGFQALGAKIIQKNGYIEAIADELIGNTIY
                     LDFPSVGATQNIMMAAVKAKGTTIIENVAREPEIVDLANILNKMGAQVYGAGTETMRI
                     EGVDHLHAVNHSIVQDRIEAGTFMVAAAMTQGNVLIADAISEHNCPLISKLIEMGAEI
                     IEEEGGVRVIGPKHILPTDVKTMPHPGFPTDMQAQMTAIQLVAEGTSVVTETVFENRF
                     QHLEEMRRMNAHVKIDGNVAIMDGNHELQGAEVYATDLRAAAALVLAGLKANGITRVR
                     NLNYLDRGYYNFHIKLQQLGADVERVDMDQTSAEKTAQTIA"
     gene            17655..17831
                     /locus_tag="IXT20_001761"
     CDS             17655..17831
                     /locus_tag="IXT20_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HAQ4860202.1"
                     /translation="MSNQRYILLTLIKILVVILLLILLFVAGTMIGYGVIGGGNPFKV
                     FQPSLWIHIRDFFH"
     gene            17966..18262
                     /gene="yidD"
                     /locus_tag="IXT20_001762"
     CDS             17966..18262
                     /gene="yidD"
                     /locus_tag="IXT20_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HAQ4860203.1"
                     /translation="MKKILIYLVRGYQRFISPAFPPSCRYYPTCSNYMIKAIQTHGAL
                     KGTVMGTARILRCHPFVKGGIDYVPLTFRLTRNKKDKKEPDYKKVVKNTGSTER"
     gene            18326..18709
                     /locus_tag="IXT20_001763"
     CDS             18326..18709
                     /locus_tag="IXT20_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ4860204.1"
                     /translation="MIEELNKKDLPWSLLLDADPDKEKVQVYVSRGSGWIWKEKEKTI
                     GVLIYVAREKEYEIVNVAVTPTYQGKGIGGKLLETAFQKLSQLASNQTRIIIRTGTTS
                     SAALHLYQKMGYVEIGRVKDYFIHH"
     gene            18878..19762
                     /locus_tag="IXT20_001764"
     CDS             18878..19762
                     /locus_tag="IXT20_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAQ4860205.1"
                     /translation="MGILVFDFGGSAVKYGCWDGKEIKGKGQFATPESWEEMKTQLFQ
                     VYKKILVSFEVEGIGISSPGVVNNEKQVIEGISAIPYIHGFNIFRDLETLFQLPVTIE
                     NDANCAGMAEFYEGAARDYQDVAFVVAGTGIGGALFTKGQINKGAHLYGGEFGLMFLD
                     GDKTFSKLGTAVQMAWRYCERKGLDKNAYTGKDVFELAETGDAIAKEEVESFYTYLTK
                     GLFSIQFSFDPEVIVLGGGVSAKEGLIDEIKSRMKKLTEQFDLHDFEPQILLCEYRND
                     ANLVGAAANYLAVRQGEN"
     gene            19879..20112
                     /locus_tag="IXT20_001765"
     CDS             19879..20112
                     /locus_tag="IXT20_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2969 domain-containing protein"
                     /protein_id="HAQ4860206.1"
                     /translation="MSKKNKDIEVRIEEVKKDIKGKNYEVTQLFIGKKMIGEILAYGP
                     KEYEIFFGEEDFGKEKSLENAIETVIRHWNLHE"
     gene            20200..20280
                     /locus_tag="IXT20_001766"
     tRNA            20200..20280
                     /locus_tag="IXT20_001766"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:20234..20236,aa:Tyr,seq:gta)
     gene            20287..20358
                     /locus_tag="IXT20_001767"
     tRNA            20287..20358
                     /locus_tag="IXT20_001767"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:20319..20321,aa:Gln,seq:ttg)
BASE COUNT         6760 a         3566 c         4425 g         5611 t
ORIGIN      
        1 tttttttgca actagcttct gtatatgatg tttacaaaga atggtacaag aaaagatcat
       61 ccttgaaaaa ggaaagctaa caataactag catcatataa gagcttttaa ggatagaaca
      121 gtagaatagg aatctatggg caagtcctct cgtataaata aaataggagg gaagtagcag
      181 catatttttc ataaagtggc tctctttctt catttttact ttggttgctc ctatttgaaa
      241 attcgagcac actcaaatac aataaaaaga aatggcaagg gagaggatta gatgaaaaaa
      301 gaagaaaaaa tcgcgatttt acaagagatt atccgaatta aatcagtgaa tggaaacgaa
      361 ggtgaagtag cagcctattt gaataagctt ttggcaaggc atgacataac aggagagatt
      421 gtttcttaca gagatggtag agataatttg attgctcgtt accaaaaggg ccaatcagga
      481 aaagtgttag gcctatctgg acatatggat gtcgtggcgg ctggtgatga atcgtcttgg
      541 acctatgcac cctttgcagc agaaatccat gggaaccgtt tgtacggacg tggtgctaca
      601 gatatgaaga gtggcttggc agcaatggtg attgccatga tcgaactgaa agagtcagga
      661 aaaccattta acggaacagt caaattatta gccactgtcg gagaagaagt gggtgaacta
      721 ggtggggaac agctcacgaa agcaggctat gtagatgact tagatgcctt gatcatcgga
      781 gagccaacca attacagttt gatgtatacg cacatgggat caatcaacta tacagtcacc
      841 tcgcacggaa aagaagcgca tagctctatg ccagaccaag gttataatgc aatcaatcac
      901 ctaaatgaat tcattacgaa agcaaatgct gaaatgaatc acctcgctga aacaatcgag
      961 aatcctgtcc taggaaaaac aatccataat gttacattga tcagtggcgg gaatcaagtc
     1021 aacagcatcc caagccatgc gcagctccaa gggaacattc gatcgattcc agaataccca
     1081 aatgataaaa tcattgcatt gctgcaatca atcgtcaatg aattgaatca agagacagac
     1141 tatcatttgg aattgatgat cgattacaac aaaatacctg tcaaagctga tccagattct
     1201 cctttgattc attccattca gcaacaattc agccagccat tacctttagt tggagcggca
     1261 gcaacaacgg atgcagccga atttacgaaa gctaaccatt ccttcgactt tgtcgtcttt
     1321 gggcctggtg tcgttacttt gcctcatcaa gtagatgagt atgttgaaat cgataattat
     1381 ttggatatga tcgaaaaata tcaaggaatt attttatcct acttagcgta agattttttt
     1441 aataaatgaa aaaacagagc cagattagca ttattttgct aatccagctc tgtttattca
     1501 gcctattttt ctttttgaat atatcaagcg ttggggcaac atagtgatga ctaatgaaac
     1561 aacgacgata gacaacaagc gatcaaggta gtctgtaccc acctgtacca agatcacact
     1621 tgttgtctga cttaaaccta acccatgtaa tagctgaacg atcatactgg atcctgagga
     1681 ggtgatcccg ccaaataact gaacggtaat gatcgaagaa acgatcgttc caggtaccga
     1741 caaaatcaaa gcgagtaata acagtttcaa gctgttttta tctttgccga gatatcttgg
     1801 caaaatcaat ccagcaagca agcctgtgag aatttggatt ggcgaataat acagagaaaa
     1861 aatatctgta gtcactccac ttaaaatacc gctggacaat cctgtcagca ttccaggtac
     1921 aggcccaagc aatgcaccag caaaaatcgt tccgatagaa tcgagataga ttggcaaacg
     1981 aagccataaa gcaatcgttc ctccaatata attgatccca atacacaaag ccatcactgt
     2041 aagtacgcga gttgccattt tgtttttatt cattttgtta gcccatcttt aacctttcca
     2101 aatctttcat aatcgtttct tcttgtgcat ttaaaagaac ggttaaaaac tttcggaaaa
     2161 aaagacgagt atctactttt gtcataattt tactgttagc aggtttctgc caaaattcat
     2221 aacgatcaac caatgtttgt ccgcgactta ttccttgagt ttctacagca gtgaagcttt
     2281 taaagccttc acagatggac tcatcgataa aataagcaac agctaatgga tcattgatga
     2341 cacatcctaa gattctttct tgtttccagt gaaagtcaaa ataaaaacga gtgatcgctt
     2401 ttagatactc tccctcttca ggattcattt ggcagcaata ttcaaggata gtcggtgtca
     2461 agacgatttc tcttgtcaca tctaacccaa ccatttctat cggttgtttg agcttttcga
     2521 atacataaga agctgcttca ggatcacacc agtaattgta ttcagcaact ggtgaacagt
     2581 taccatggct tttgtaagtt ccgcccattg agacaaaacg atccatatgt ttcccaacag
     2641 atgggctgtt tttcaaagca gaagcgatat tggtcaaagg ccccaaagca atgactgatg
     2701 tatctgtttt ttctttgaaa taatcagtca gaaaatcaac tgcatggagc gattctgcct
     2761 gtttggctaa gaccatcggg aaatttgtgt cccctaatcc atccaaccca tgagtgtctt
     2821 gcgcactaat gaaaggttgc ttcaaaggtt tttccattcc ttgataaaca gggatatcta
     2881 aacgatcaca gcgctccaaa catttcagca cattctgcgt accgatatct acagggacat
     2941 tcccgcaaac gacggtaata gcaactactt cgatatcagg ggatttgaca gctagtgata
     3001 aagctaaagt gtcgtcgatc cctggatcgc aatcaataat tactttacgc attatttcca
     3061 atctcctttc tttagagata cttagttttt tattctggga agtttccgaa ccagtccaag
     3121 acgagtgtct tgtttataat gcacgaaatt agtatactgg cttttattgt gaatgtaaat
     3181 gctctcttac aagtaaatct attgagaaga actttaagta aatgatacaa tgaaaaagaa
     3241 atggaattta cgtgtgccga agaaaaagaa tcctattgat cggtatattg tataataaat
     3301 agattatagt ttggcagaaa gcggcgggtg tacgatggtt attttttatt tcattcttat
     3361 tctgatattt ataggaatca gttaccattt tctttcttat ttttatgcaa agaaattagt
     3421 aagagcgggg gtacagagag gctatgactg gtatgaagaa actgggcacc gcttgatgga
     3481 tcctgctgca gtcacgtcag tagaaaaaaa gcgacaaaaa ttagaagaag agtgtgaaag
     3541 cttctgggaa caaggaatac cgaaaacgat caaaagttat gacgggttga aactagctgg
     3601 acagatgttc ctccaaccaa ctcagcagaa taaatgggtc atttgtgtac atgattatcg
     3661 aagtacaggg aaaagagata tgtctcatat aggtaaaaga tatgcagaga aaggattcaa
     3721 cgttcttata cccgatttac gtgcgcatgg agaaagtgaa ggagaaatca tcggaatggg
     3781 atggctagac cggctagatt taattgcatg gatccaactc atcttagatg aacaaccaga
     3841 tgcatccatt attttgcacg gcagttcgat gggcgcttct actattatga tggcaagtgg
     3901 tgaaaagctt ccatctgcag tcaaaggatt tatcttagac agcggatatg tttctgttta
     3961 tgcggaattt cggtatatgt taagtaaaat cacggtcttt cctaaaaaaa tgatcatgag
     4021 atatgcgaat cactatgctc agaaatacgc aggttattct ttaaagcaag catcagctac
     4081 aagacagctt ggtagtaacc atttgccttt gttagtcatc catggtgaaa gagatcattt
     4141 tgttcctaca gaagcagcat acacgattca aaacgctacg gcaggggata aagcattact
     4201 gcttgttcca gaagcagaac atttggaagc atcgatgaaa gatccgaaaa cctattggac
     4261 agtgattttt tcatttattg aacaaagaat aacgatggat tgattttctc ttcctttttg
     4321 taagcaacac gaggataaat aattgtttat agaccatgag actgttaaaa taaggtagga
     4381 ataaaaaaat atcagttatt tgaggagtgc ggcatatgga aatcaaagag gaaaaaaatc
     4441 gtcttgcttt attcaatgat gagcaacaag aaatcggaga aatgacatgg tcggatgcgg
     4501 ggccagacat catgatcatt gaccatacat ttgtcgatcc agcatatcgt ggtcaaaaat
     4561 tagctgaaaa gctagtgtat actggagtag aactagcaag acgagagggg aaaaagatta
     4621 tcccttcatg cccttacgca aaaaaagagt ttgagaagaa accagagtat catgacgtct
     4681 tacgccaatc ataataataa cagctgaaag atttgcactt tcagctgttt ttccatttat
     4741 atagagttgt cacggagttt tatctatgat aaagttaaag agacgatatc ttttgggaag
     4801 gaggctgcta tatgtataat ttaattttgg gaatcgtgat cgttttatcg attgttatgg
     4861 tcattgcgat tatgatgcag ccaagcaagc aaaatagtgc agcgagtgca tttaccggcg
     4921 gtgcggatca actattcggc aaacaaaaag ctcgcggctt tgaagctgtc atgcagcgat
     4981 caactgcagt catgggagct gtctggatga tcttattatt tgtcctagca tttttatctt
     5041 cgaagtagtc gaataagcct ctcgcacaac ggcgggcggc tttttttatt gcaaagagaa
     5101 cgcttaagaa gaagttatga aagaaactat ggtaaaatga ggcgaaagag gtgaaacgag
     5161 tgaaacaatt accaaaaccc ctttatgcaa aacatggaaa acgagcagtt ttgctgcttc
     5221 atgcgtactc aggaagtcca aatgatgtcc ggatgctcgc acggttttta gaaaaagcag
     5281 attatacagt ctatgcgccg ctatttaaag gacatggaac gatggagcca tatgatatct
     5341 tgcaagaaaa agcagaaagc tggtgggcag atactaaaaa agctatccat tttttacagt
     5401 ctgaacagtt ttctgatatt gccgtattgg gattatcgat gggtggcatc tttgctgtac
     5461 gggcactaga agaagaatca gtaattggcg gcggtttttt ctgttcgccc ctgtctcctg
     5521 tagaaacaaa cgtgcctgaa aattttgaaa aatatgtacg tcaagtgctg aagatagcag
     5581 gcaaatcaga aaaagaaatc aatgaaaaag ctgtgtctta ccgctcgtta gctgaacatc
     5641 aattaatgga tatacaagac caagcagcaa tcgcagaaag tagactatca gatatccaac
     5701 agccaatatt tttagctcaa gctgggaaag atgaaatgat cgacccaaat ggtgtatttg
     5761 aaacggcacg caaattgagc cgccaacgag tgacacttca atggtatcct gaaagcggtc
     5821 atgtgattac agttggcaca gcaagacgcg aattagaaaa agacgtgttg gaatttttag
     5881 aaaaattacc ttggaatgag gaataaatat gacaaaagaa acaataaaag agaaaattct
     5941 ctttttcatg gaaagcagtc gcaaacgtag tttttctatg gaagaaatcg ctgaaggtct
     6001 aggctttcaa aaaagcgatg attttaaatt actagtacaa acgattgctc aaatggaaag
     6061 agaacagagt atcgttttca ataaaaaagg aaaagtcaaa cttcctttaa aaccgatctt
     6121 ggtcgaaggg acatttcggg caaatgaacg aggatttgga tttgtaacga ttgatcccga
     6181 agaagatgat atctatatcc cgaaagaagc gactggcttt gcaatggatg gggataccgt
     6241 agcaattgat atcatgaagc gagcaaatac agatatggat cgcggagcag agggaaaagt
     6301 agtggagatt cgagaccgag cgactactca gttagccgga gaatttgttg cttattcaga
     6361 cgaagagata caggaaacgg atctttatgg tgtggtcatc ccgaaagata gaaagcttaa
     6421 ccaattcaaa gtgtatgcag cggcagaggg agttcgtcca gttgacggaa gtattgtatt
     6481 gatcgaactg acccattacc ctgaaaaagg gtatgcaact agtttagaag ggataatcaa
     6541 gcaagtgatt ggtcataaga atgaccctgg catggatatc ctctcgattg tcgtggctaa
     6601 cggtattcct acaaaatttc ctgatgatgt gttatctgag gcagatgaag ttccagacca
     6661 gatcagcgaa aaggatattg ttggacgcag agatttaaga gatcagctca tcgttacgat
     6721 tgatggagaa gatgcaaaag atctagatga tgcagtgacg gtcaaaaaat tagaaaatgg
     6781 taattatttt cttggagtac acattgcaga tgtatcttac tatgtgacgc aaggcagtca
     6841 actggacaga gaagcatacg aaagaggaac aagtgtctat ttgaccgatc gagtgattcc
     6901 gatgatccca caacgtttat ccaatggtat ctgttcattg aatccccatg ttccacgact
     6961 caccatgagt tgtgaaatgg agatcgatcc aaatggacaa gttgtctctc atgagatttt
     7021 tccaagtgtg atccaaacaa cagaacggat gacctacaca gctgtcaatc aaatcttaga
     7081 agatcaagat gaagaaacga tgcaacgtta tgctcagctc gtgcctatgt tccaagaaat
     7141 gggtgaattg catcgaatcc ttgaaatgat gcgaatcaaa cgtggagcga tttcttttga
     7201 ggatcgtgaa gcgaagatcc ttgttgatgc tgaaggacac ccaaatgata tccagctaag
     7261 aagcagaggg ataggtgaac gcttgatcga atcctttatg cttgctgcca acgaaacggt
     7321 agctgaacat tataataaat taaatcttcc gtttatttat cgaatccatg aacagccaaa
     7381 agaagaaaaa atgcagcgct tctttgattt tgcttcagct ttgggcattc tggtccgagg
     7441 aacgaaagga acgatcacac caaaagattt acagcatgtg atcgaaggag tagaagacaa
     7501 accagaagct gcagttatca atactatgtt gctaagaagc atgcaacaag cacgctattc
     7561 agaagacaat tttgggcatt atggcttagc ggcagattac tacacacact ttacatctcc
     7621 tattcgacgc tatcccgatc taatcgtcca tcgtctgatc cgttcttaca gtgaagataa
     7681 aagtgaaagc aatcaaagtt cgtgggctgc ggaacttcct gaaattgcag atcatagttc
     7741 taagatggaa cgtcgtgcag tagaaacaga acgtgaagta gattccatga agaaagcaga
     7801 attcatgatg gacaaagtgg gagaagaatt cgatgggatc atcagctctg ttgtgaaatt
     7861 cggcctgttt atcgaactgc cgaatacagt agaaggattg atccatatca atgaattgaa
     7921 acaggactac ttccattttg ttgaaaatca tttagctctt gtaggtgaaa gaactggtct
     7981 gacttttaaa atcggacaaa aagtgcgcgt aaaagtagtc aaagctgatc cagatgaacg
     8041 agcagtggat tttgaactcg tttccgcaga agaggtcact gcgttggaac gtccaaaagc
     8101 acgcaatgga aaatctggaa aacgagaagg aaaacaagca ggcaaacgct cagaaaaaaa
     8161 tggcaagaag aacaaacgat atagtgatag taagcaaacg ttcggtaaga aaaaagggaa
     8221 aaagccattt tacaaaggtg tcaaaaagaa gaaaaagaaa aaataaaatg agttggagga
     8281 aacagctatg ccaaaaggcg agggaaaact aattgcacaa aacaagaaag ctcgccatga
     8341 ttattcgatc atcgacacga tggaagcagg gatggtcttg caaggaacgg agatcaagtc
     8401 gatacgaaac agccggatca atctgaaaga tggatttatt cgcgtccgca acggggaagc
     8461 tttcttgcat aatgttcata tcagtcctta tgaacaagga aatattttta atcatgatcc
     8521 gttgcgcacg agaaagttat tattacacaa gaaacaaatc gttcgtcttg aaaacgaatt
     8581 gaaaaacact ggaatcactg ttgttccttt aaaagtctat attcgtaacg gctatgccaa
     8641 ggtattgatt ggtctggcaa aagggaaaaa atcgtatgat aaacgggaag atttgaaacg
     8701 aaaagatatc gatcgacaaa ttgatcgaac attaaaaaat ttctctagat aatttcatta
     8761 tcctttttcg catattcggt gcttttgctt ttacttaatg ataaaatttg ttagcattta
     8821 atctgataaa acatgattat cacaggtttt ttcttagatc aggagcaccg tgcatgggga
     8881 aaaattcgct tcgtttttga ctgaaataaa aaagtgtcta attttcatga aaatgttttg
     8941 aattttctgt ttcatggtgg tatgataccg tgtggttaga aaccgattgg aaaaaaccag
     9001 atacgcacat gaattgattt tatgtgaatt tcggggaaaa gaggtgaaat ggattggatg
     9061 aacgctcgct aacgtttcac attggaccaa tttggtttga tggtacagtt tgtataatgg
     9121 tgttactgac ctgtttgatt gttttcttct tagcttactt ttttacaaga aatcttaagt
     9181 tgaaaccaac aggcaaacaa aatgcgatag aatgggtcat tgattttagt cgaggaatcg
     9241 taacagataa tctgcctaga aaagaattga gcaatttcca tttgttggca tttacattgt
     9301 ttttatttgt ttttgttgca aacaacatag gtttagtaac aaaaatcgtc ttacctaacg
     9361 aaacaacact ttggaaaagc ccgacagcag acccgtttgt tactttaact ttggcattta
     9421 taatgatctt acttactcac ttattcgggg tgaaaaagtt aggcttcaaa ggatatttcg
     9481 taaattcgtt cttgaaaccc tatagtttta tgtttccgat gaaactgatt gaagaattta
     9541 caaacttatt aacacttgct ttacgtcttt atgggaatat ttatgcgggg gaagttttgc
     9601 taaccttgat tgcaaaaatg atgacaagct taggctggtt ctcattacca ttagcaattc
     9661 cattagagat ggtctggatc gcgttctcct tgtttattgg gagcatccaa gcatttgtct
     9721 ttgtcacttt gtcaatggtt tatatgagcc ataagattga agtagaagaa taatactcgg
     9781 ttaaaaaaca aactactatt tatctatttt aggaggaaaa caattatgaa ttatatcgca
     9841 gcagcaatcg caatcatggg agcagcaatc ggagccggtt atggtaacgg tcaagttatc
     9901 tctaaaacaa tcgaatcaat ggctcgccaa cctgaaatgt ctggtcaatt ccgtacaaca
     9961 atgtttatcg gggtagctct agttgaagct gttcctatct taggggtagt tatcgccttg
    10021 attctagttt ttgccgttta attgaatcag tattaaggca gaaacaacat cgtttgttac
    10081 tgctttttta catggaaact agaaatactc caaagaaagg aaggctcgcc tatgctgaat
    10141 caattggcaa ttgcagaagt tggcaatgcg atgttaggta atatcatcgt tgtcagcggc
    10201 tcatttttga tactactggc tctcttgaag cactttgctt ggggaccaat cagcgatatt
    10261 ttgaaaaaac gtgaagacaa gatcgccaat gatttagatt ctgcagaaca atctcgcatc
    10321 aactcagcga aaatggaaca agaacgcgaa caacaattgt tagcctctcg ttctgatgca
    10381 gctgatatca tcaaaaatgc gaaagaaagt ggagaattaa gccgccaaaa tattttgaag
    10441 gatgctcaag aagaagcagc tcgtctaaaa agcaaagccc aagctgatat cactgtagaa
    10501 cgtgattcag cgctgaactc tgtaaaagac gacgttgcag aactctctct tcaaatcgcg
    10561 gaaaaaatct tgaacaaaga attatctcca gaaatgcatg aatcattaat caatcaatac
    10621 attgaaggtc taggttcttc aaatgaaact agataaatat acagtaggaa gacgttacgg
    10681 gaaagcttta tttgaactgg cgatcgattc aaatcaagca gaagaaatct atcaagatct
    10741 cgtaagttta cgtcaaatct atgaacaagt acctggatta gggaatatgt taagtgatgt
    10801 tcgtctagaa cctcatgaaa aacgagaaat catggacaag cttgtcagcg gttatgatgg
    10861 aatcgttaaa aactttttag aagttgttta tgagtataac cgaatggatg accttttgtt
    10921 catgattgaa gaatatgaac gtcgatatga tgaacatcaa gggttactat tgggaagtgt
    10981 cacaacagca gtcccacttt cagacgaaca acgaatacaa ttagaaaaga atgttgcaaa
    11041 aacaatggac tatcaatcag ttgaattaaa acaaatcgtt gattcttcta ttattggtgg
    11101 cgcgattgtt gaagccaacc atcgagtaat cgacggaagc attcgcacgc aattagaaaa
    11161 aatgcgtaat caattaaaca gatagtacaa agaggtaggt gaatcgaatg gccatcaaag
    11221 cagaagaaat tagtgccttg attaaagaac aaattgaaaa ttatcaaaac gtgctttcag
    11281 tcgaagaaat cggtaccgta acttatgtag gtgacgggat cgctcgtgca catggtttag
    11341 aaaacgcgat gagtggtgag ttgcttgaat tttcaaacgg ctcatacgga atggcgcaaa
    11401 acttagagtc aaatgatgta ggtatcatta tccttggcga tttcgagtcc attcgtgaag
    11461 gagataaagt aaaacgaaca ggtaagatca tggaagttcc agttggagag gccttgatcg
    11521 gtcgggtagt caatccgcta ggtcaaccaa tcgacggact aggtgaaatc gttactgaca
    11581 aagctcgtcc agtagaagcg atggcaccag gtgttatgca acgtaaatct gttaacgaac
    11641 caatgcaaac agggctaaaa gcgatcgacg cccttgtgcc aatcggacgc ggacaacgtg
    11701 aattagtcat cggtgaccgt aaaacaggga aaacttctat tgcgattgat acgatcatca
    11761 accaaaaagg tcaagatatg atctgtatct atgtagcaat cggacaaaaa gattctacag
    11821 ttcgtacaca agttgaaaca ttgaaaaaat atggcgcaat ggattacaca atcgttgtga
    11881 atgccggtgc gtctcaacca gcaccattac tttatatcgc accatatgct ggtactgcaa
    11941 tgggtgaaga attcatgtac aacggtaaac atgtattgat catttttgat gatctttcaa
    12001 aacaagctgt tgcttatcgt gaactttcct tgctacttcg ccgtccacca ggtcgtgaag
    12061 cttatcctgg ggatgttttc tacttgcact cacgtttatt agaacgtgca gcaaaattaa
    12121 gtgatgatct aggcggcggt tcaatgactg ctttgccatt tgttgaaaca caagctggag
    12181 atatctctgc ttatatccca acaaacgtta tctcaatcac tgacggacaa atcttcttag
    12241 aaagcgactt gttctacgcg ggtactcgtc cagcggttga tgccggacta tctgtctctc
    12301 gtgtaggggg ttctgcgcaa atcaaagcaa tgaaaaaagt tgctgggact ttacgtctag
    12361 acttagcaag ttatcgtgaa ttagaagcat ttacacaatt cggttctgat ttggatgcgg
    12421 caacacaagc aaaactaaat cgtggacgcc gtacagtaga aatcttgaaa caaaaactgc
    12481 atgcaccgct agctgttgag aaacaagtag tgattttgta tgccttgaca catggattct
    12541 tggacagtat ccctgtagac agtattttag acttcgaaca tgaattattc gaatatctgg
    12601 ataccaatca ttcagatatt tttgaaacaa tccgtacaac aaaagatctt ccagaagaag
    12661 aaacgttgaa cagtgcgatt caggaatata aagacatgtt cttagctaca aaaggaaata
    12721 attcctctac agaagataaa ttgaaatcca ttcaaaatgc atagtgaggt gagatgaatg
    12781 ggtgcttcat taaatgaaat caagacacgt atcgcttcaa cgaaaaaaac gagccaaatc
    12841 acgcgggcaa tgcagatggt gtctgcttct aagttgacaa aatctgaagc ttcttcacag
    12901 aagtttcagg tttatgcaac aaaagtaaga gaaatcgtta cgcatttgac tgctactcag
    12961 ttaaatgata ttgcctcaga aaatcctaga ggggatatca attacaacag catgctgctc
    13021 agccgtcctg tgaagaaaac cggttatatc gtcatcactg cagatggcgg tctggtcggt
    13081 ggatataaca gttcgatatt gaaacaaacg atgtctattt tagaagaaga ccaccaatca
    13141 cccgatgatt acgtgatgat cgcaattggc ggaactggtg cagacttctt taaagcacgc
    13201 ggtatcaacc tagcatatga gctgcgcaat ttatctgatc agccaagctt tgatgaagtc
    13261 cgcaaaatcg taaatatggc aacaactatg taccaaaacg aagtattcga tgaattatac
    13321 gtatgttata accatcatat caattcgcta actagtcagt ttcgtgtaga aaaaatgctg
    13381 ccaatctcag acttagatcc agaagaagca acgactttcg aacaagaata tatttttgaa
    13441 ccttcaaaag aagaaatctt gtctcagtta ttgcctcagt atgctgaaag tttgatctac
    13501 ggtgcgatcg ttgacgccaa aacagcagaa catgctgctg gtatgacagc aatgaaaaca
    13561 gcaactgata atgctgctaa tatcattgat gagctgacgg tttcttataa ccgtgcaaga
    13621 caaggggcta ttacccaaga aattaccgaa attgttgccg gagcttcggc actagaatag
    13681 tattgagatt ggaggaaaaa agatgagttc aggcaagatt gttcaagtaa tcggtcccgt
    13741 tgtcgacgtg gaattttctt tagaccaatc cttaccagat atcaacaacg cattagttgt
    13801 ttataaaaat gacgaaaata aatcaaaagt tgttcttgaa gcagcgttag aattagggga
    13861 cggagtgatc cggaccattg cgatggaatc aacagatggt ttacaaagag gaatggaagt
    13921 cattgataca ggcaaagcaa tctctgttcc tgtaggtaaa gaaacattag gtcgcgtatt
    13981 caacgtacta ggagatacga tcgatttaga aacacctttc ccagaagatg cggaaagaag
    14041 cgaaattcat aaaaaagcac cagcctttga cgaattaagt acaagtacag aaattttgga
    14101 aacagggatc aaagttatcg atttgcttgc cccatattta aaaggtggga aagtcggact
    14161 attcggtggt gccggtgttg gtaaaaccgt actgatccaa gaactgatcc ataatatcgc
    14221 ccaagaacat ggtggtattt ctgtatttac cggtgtaggt gaacgtactc gtgaaggtaa
    14281 tgacttgtat tatgaaatga aagattcagg agttatcgaa aaaacagcca tggtgttcgg
    14341 acaaatgaac gaaccaccag gtgcacgtat gcgtgttgct ttgactggtt tgacgattgc
    14401 ggaatatttc cgtgatgtag aaggtcaaga tgtactgttg tttatcgaca acattttccg
    14461 tttcactcaa gctggatctg aagtatcagc cttgttagga cggatgcctt ctgcggttgg
    14521 ttatcaacca acattggcaa cagaaatggg tcaattgcaa gaacgtatca catctacgaa
    14581 aaaaggttcg atcacatcga tccaagcaat ctatgtgcca gccgatgact acactgaccc
    14641 ggcgccggca acagcattcg cccatttgga tgcgacaacc aacttggaac gtaaattaac
    14701 agaacaaggg atttatccag cggtagatcc attggcttct tcttcaagtg ctttagcacc
    14761 tgaaatcgtg ggagaagaac attataaagt agcaacagaa gtccaacacg tattgcaacg
    14821 atatcgtgaa ttacaagata tcattgctat cttaggtatg gatgaattat ccgatcaaga
    14881 aaaagtgtta gtttctcgtg cccgcagagt ccaattcttc ttgtcacaaa actttaacgt
    14941 ggctgaacaa tttactggtc ttccaggttc atatgtccct gtagaagaaa cagtgaaagg
    15001 attccgcgaa atcttggaag gcaaatacga tgatcttcct gaagaagctt tccgaagtgt
    15061 tggccgaatc gaagacgtcg tagaaaaagc gaaaacacta ggctactaga aaggggtgcc
    15121 ccatggatca atatttaaca gtaaatgtgg taactcctgc cggtttggtc tatgatcacc
    15181 atgcagctat cgtcgttgca cgtacgacag ctggtgagct tgggattttg cctaaacatg
    15241 caccaatcat cgttccattg acgattgatg aagttcgtgt aaaacgaaca gattcagata
    15301 cccacgtcga ttggatcgct gttaatggcg gaatcatgga agttcgtgat aatgttgttt
    15361 caatcgttgc tgatagtgcg gaacgtgaac gtgatatcga tgtatctcgt gcggaacgtg
    15421 caaaacaacg cgcagaacgc caaatcgaag aagcaaaagc aaaacaagat acgaacgaat
    15481 tgaaacgggc aactgttgcg ttgcaccgag caatcaatcg tatcaaagta tctaagcact
    15541 cataaaatga gatgcggatg tttatccgtg tttaagaaca gtaaaaagag gttatgacag
    15601 aagtggtcag cttcaagaca taagaaggaa tttacgaaaa ttgcttctca aatttttgtg
    15661 aaatctcagc ttatttttcg aagaagctac ttctggaaca ccgtatatca gaggttgtga
    15721 aatcagcgtt ttgtcctgag tattagagaa acaaatggaa aatagttcct catattttgc
    15781 cgttttttga gctaataccg agggtcagat gattgaacac cgtttatttg gaataaggga
    15841 ctgcgacaag acttttgtct cagtcccttt ttttgcatat ttttagcatt tatttcctaa
    15901 aaaatgtaag gggattccta tatttttaag tatacttgtt tttcctttta tacattttat
    15961 ggtattatca ttttattagt tattataaaa caatcgttag aaaggacgtc attatgcaag
    16021 tatacggaat tgatgcggtg cttcgaatcg tgagtcattt ggcgtttatc tacttagcct
    16081 tctggtcttt acgttcgtta cgcattgaaa cttttttcag accaatgcat aatacgcaaa
    16141 ttagaatggc aatcgtactt ttttcaattt ttttagggta cactgcaagt aattttttct
    16201 tagaagttat cgctttatgc cgaaatcttt tcgttagcct gtaaatttta aaaaaaggta
    16261 ctattttaaa agcaaggatg ttataataga aaagattttg agtaagtgta tgctcattgg
    16321 aattttttgg agggaacaac atggaagaga tcatcgtaag aggtggcaat caattaaatg
    16381 gaacagtaag aattgaagga gctaaaaatg ctgttttgcc tattttagct gctagtttgt
    16441 tagcagaaga aggtattacg acattagaca acgttccgat tctttctgat gtcttcacta
    16501 tgaatcaagt gattcgtcac ttaaacgtag atgtagattt tgatgaacaa aagaatcaag
    16561 taacaatcga cgcttcaaga caattagaaa tcgaagcgcc ttatgagtat gttagtcaaa
    16621 tgcgtgcttc aatcgttgtg atgggtccgc tacttgccag aaatggacat gcaaaagtag
    16681 ctatgcctgg tggatgtgcg attggtaaac gaccaatcga tttgcattta aaaggatttc
    16741 aagctttagg agcaaaaatt attcaaaaaa acggctatat cgaagcaatt gcggatgaat
    16801 tgatcggtaa cacgatttat ttagatttcc ctagtgtggg tgctactcaa aatatcatga
    16861 tggctgccgt aaaagcaaaa ggaacaacca tcattgaaaa cgtagctcgc gaaccagaaa
    16921 tcgttgattt agcaaacatt ttgaataaaa tgggcgctca agtctatgga gctggtactg
    16981 aaacaatgcg tattgaaggc gtggatcatt tacatgcagt caaccactca atcgtacaag
    17041 atcgtatcga agcaggtacc tttatggttg ctgctgcaat gacacaaggc aatgtgctga
    17101 ttgcagatgc tatctctgaa cataactgcc cattgatttc taaattgatc gaaatgggtg
    17161 cagaaatcat tgaagaagaa ggcggagtcc gtgtcatcgg accaaaacat atcttaccta
    17221 cagatgtaaa aacgatgcct catcctggtt tccctacaga tatgcaagct caaatgacag
    17281 ctatccaatt agttgcagaa ggtacaagtg tggtgactga aacagtgttt gaaaatcgtt
    17341 tccaacattt agaagagatg cgccgtatga acgctcatgt caaaatcgac ggaaatgtag
    17401 caatcatgga tggaaaccat gaattgcaag gtgcagaagt atatgcaaca gacttgcggg
    17461 cagcagcagc attagtttta gctggcttga aagcaaatgg aatcacacgt gtgcgtaact
    17521 tgaattatct agaccgtgga tattataatt tccatatcaa actacagcaa ttaggtgcag
    17581 acgtcgaacg tgtagacatg gatcaaactt ctgctgaaaa aacagcacaa acgattgctt
    17641 aaggaagtgg ctgaatgagt aaccaacgat atatcttatt aactttgata aagattttag
    17701 tggtgatctt attgcttatc cttctttttg tggccggaac gatgatcgga tatggtgtca
    17761 tcggtggcgg caatccattt aaagtattcc agccaagttt gtggattcat attcgagact
    17821 tttttcattg ataaataaga atcacagtgg ttctagtcga tatgaagtga gccatgaagc
    17881 agaacatgat aaaagaaaac agaagggaac gttgacgtta gtcgcgatcc ctttttttag
    17941 taaaagaaaa gaaggaatct aattgatgaa gaagatattg atttatttag ttagaggata
    18001 tcagcgcttt atttcgcctg catttccacc tagctgccgt tattacccaa cctgttccaa
    18061 ttatatgata aaagcaattc aaacccatgg tgcgctaaaa gggacggtaa tggggactgc
    18121 acggatctta cgctgtcatc cttttgtcaa aggcggtatc gattatgtac cattgacatt
    18181 ccgattaacg cgaaataaga aagataaaaa agaaccagat tataaaaagg ttgtgaaaaa
    18241 tacgggtagc actgagcgat aaagaaaaag aggaaattta ttgtcaaagt aacaactccg
    18301 caactactta tatgggggac aaaaaatgat cgaagaacta aacaagaaag atttaccatg
    18361 gtccttgtta ttagatgcag atcctgataa agaaaaagtt caagtatatg tttccagagg
    18421 gagtggctgg atatggaaag aaaaagaaaa aacaataggt gtactgatct atgtggcaag
    18481 ggaaaaagaa tacgaaatcg tcaatgtagc agtaacccca acgtatcaag gaaaggggat
    18541 tggcggaaaa ttacttgaga cagcttttca gaagctttca cagctagctt caaaccaaac
    18601 tcggatcatt atacgtactg gcactactag tagcgctgcg ttacacctct atcaaaaaat
    18661 gggctatgtg gagattggtc gagtcaaaga ctattttatc catcattaat ctgagcccat
    18721 ttttgaaaat ggagatcaat taagggatca agtgatttta gcaattcact tacctaaaaa
    18781 tagctttaac taataaactt tcaattcgag aaaatgcttt caaatgctaa atagatacat
    18841 tacaatagct ttagcaaaca aaaaggagaa tatttttatg ggaatattag tttttgattt
    18901 tggtggatcg gctgtcaaat atggttgttg ggatggcaaa gagattaaag gtaagggaca
    18961 atttgcaacg cctgaatctt gggaagaaat gaaaacgcaa ttatttcaag tttataaaaa
    19021 aatactcgtt tcttttgaag tcgaaggaat tggaatcagc tctcctggag tagtgaataa
    19081 tgagaaacag gtgattgagg gaatcagtgc gattccttat attcatggat ttaatatttt
    19141 ccgagatttg gaaactctgt ttcaactccc agtaacgatt gaaaatgacg ccaactgtgc
    19201 cggaatggct gaattttatg aaggtgctgc ccgtgattat caagatgttg cttttgttgt
    19261 ggcaggaact gggattggcg gggcgttgtt cacgaaagga caaataaata aaggggcaca
    19321 tctttacggt ggagaatttg ggttgatgtt cttagatggc gataagacat tcagtaaact
    19381 tggtacagcg gtacagatgg cttggcgtta ttgtgaacgc aaaggtctcg ataaaaatgc
    19441 ttataccgga aaagatgtct ttgaattagc agaaactgga gatgcaatag caaaagaaga
    19501 agttgagagt ttttatacgt atcttacaaa gggattattc agtattcaat tttcgtttga
    19561 tccagaagtg atcgtattag gcggcggggt ttctgcaaaa gaagggctca ttgatgagat
    19621 caaaagccga atgaagaagc tgacagaaca attcgatctg catgatttcg aaccgcagat
    19681 tttattatgt gagtacagaa acgatgctaa tcttgtaggt gcagcagcaa actatctcgc
    19741 tgtacgtcaa ggagagaatt aagagcttta gagaaggtgc agcttgcata aaagaagaga
    19801 atgaaatgaa taggagattt ttacctaagg aatgttttat ttgctgcagt atgataaaat
    19861 atctgagagg tgaaaatgat gtcaaaaaaa aataaagata ttgaagtgcg tatagaagaa
    19921 gtgaaaaaag atatcaaagg taagaattat gaagtcactc agttatttat agggaaaaaa
    19981 atgattggtg agatcttagc ctatgggcca aaagaatatg aaattttctt tggtgaagaa
    20041 gattttggaa aagaaaaaag tcttgaaaat gcgatagaaa cagtgattcg acactggaat
    20101 ctgcacgaat aaaaaaatat caaaaaaact ttcaataaag ggttgcaaaa atctggaaaa
    20161 ttaggtatta taactaagtc aggttgatac aaacctttcg gaggagtagc gaagtggcta
    20221 aacgcgacgg actgtaaatc cgttccttag ggttcagtgg ttcgaatcca ctctcctcca
    20281 tttcattggg gtatagccaa gcggtaaggc aagggacttt gactccctca tgcgttggtt
    20341 cgaatccagc taccccagta ta
//
LOCUS       DACGLE010000044        20124 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0534
            SAMEA3304449-rid9560453.denovo.048, whole genome shotgun sequence.
ACCESSION   DACGLE010000044 DACGLE010000000
VERSION     DACGLE010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304449
            Sequence Read Archive: ERR985064
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20124)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20124)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702225.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702225.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 20:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,045
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 27 of 67
            Pseudo Genes (incomplete)         :: 25 of 67
            Pseudo Genes (internal stop)      :: 19 of 67
            Pseudo Genes (multiple problems)  :: 4 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20124
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304449-rid9560453.denovo.048"
                     /strain="VREN0534"
                     /db_xref="taxon:1352"
     gene            261..1130
                     /locus_tag="IXT20_001768"
     CDS             261..1130
                     /locus_tag="IXT20_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extradiol dioxygenase"
                     /protein_id="HAQ4860207.1"
                     /translation="MKDFQLGAATYLKSVAIRVKDRDKMIDFYKNVVGFDLKREENEL
                     AILGTEEAGSEMLLLEESPRANDHMGVIKKLNRFSLIIPSTEEMADILCRIRKMNYPI
                     KEALEDHGRMGILLVDPEENELEIYHEENKNDQREIPETMDQEALLRKSEEKFSKLST
                     GSYFEKIHLNVTNLTKERQFLNHVLGLKVHNEEEGIHVLNEGDFHVGLTEASGGAIDL
                     PTDEVIGLDFLKFMIDTESMEGLIAHLQQLSVDFFLDKKHTVLTIYDAIGIEWWFVSE
                     PKEKNKTDIRCDA"
     gene            1339..2364
                     /locus_tag="IXT20_001769"
     CDS             1339..2364
                     /locus_tag="IXT20_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HAQ4860208.1"
                     /translation="MYPHTKQLILKKMEEGFYPGVAVAFIKENQVETFTAGFAQVVPE
                     KQLMTEDLLFDAASLTKVVATTSLVLRLLEDGKIELDQPLHKYLPEFENQTITLRHLL
                     THTSDIKTWIPNRDKLSQEELIAAYLKLEPGEQLGKEVKYTDAGTILLGLMLEEIYQE
                     TAVEIFQEQVLEPLGMMNSLFLPFPSKKIVPTEQLPSGEVLRGQTHDPKARVLAEHAG
                     NAGLFIDLKDSITFVQMYLNKGKIRNGRFLEEETIDSLLQDQTPKKNGYRSLGWDLKK
                     DVEDGHAILFHTGYTGTFFLIDIKTQEALIFLSNRVHPVDHRAEYLICRDEIVENFLK
                     EKAVLPK"
     gene            2403..3353
                     /gene="manA"
                     /locus_tag="IXT20_001770"
     CDS             2403..3353
                     /gene="manA"
                     /locus_tag="IXT20_001770"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HAQ4860209.1"
                     /translation="MQEPMFLKPVFQEKIWGGSRLRSVFGFDIPNDKIGEDWAISAHP
                     HGVSVVENGEFKGKRLDELWSEHKELFGHPSEPVFPLLIKILDAEDDLSVQVHPDDAY
                     GMKHEGELGKTECWYIIDAEPGAEIIYGHHAKTREELAEMIKDGRWDDLLKKVPVKKG
                     DFFYVPSGTIHAIGKGIMILETQQSSDTTYRVYDYDRKDAQGQTRELHIQQSIDVTTV
                     PAKTPELQIKEVRKGNSSIVTYLETEFFNVYEWDIKGITSFKKQAPYTLMTVIEGAGD
                     LVVDGKTYPLEKGTSCIIPNGVSEWAVQGELAIIASEPGK"
     gene            3447..5150
                     /locus_tag="IXT20_001771"
     CDS             3447..5150
                     /locus_tag="IXT20_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ4860210.1"
                     /translation="MEYKWKANNSSRVSMKQLRKAGHHLNEMIMPNLSLFIAWGILSL
                     ATKGVSGDLHTTLEEVCHWMIQLLLPILISYSGGKSVGGQKGAVAGAIASLGLIARSN
                     APQIVGAMIIGPLAGIVYEQFVSRFHVKFKAGYEMLVSNLLVGLTGSVICIIGIVFIE
                     PVLNSVHLLLASVVSWLVTMNLLPLVSLILEPLKVLFFNNAINHGVLTPLGIEFSQTV
                     GYSSLFLMEANPGPGLGILLSILCFAEKQEKVNASGALMIHAIGGIHEIYFPFVLLRP
                     YLFLAVMAGGASGTLIFQWLDTGLAAPVSPGSLLVIFANAPYPQLAGVSIGIAISTFV
                     TFICAAILLKLPNKEKENKTVKERGEILEPSIIHSVIFACDSGMGSSAMGAALLKKKL
                     AAEKIDLSVGYSSIYELNNQVNQLVIVQHELAGLAREKVPQSQIYVLDHFLETDILLS
                     RIKEAIQFPKREEKKEIADITEQKTSPANIVFLYKNNRRGSQTMGMTILSQLAKKAKK
                     QFVISKESIEKIKIQEETIYIAQRELAVTEQLEEKIPRLVLVEHWVATNEYEKLVKGE
                     I"
     gene            5152..7185
                     /locus_tag="IXT20_001772"
     CDS             5152..7185
                     /locus_tag="IXT20_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HAQ4860211.1"
                     /translation="MYLSPREKQLLAEFLSSPSPVSIQKMMNLLKVSKRTIYRELDNL
                     EVSLKSVNASLKKAARGSFVIEASEEVLEQLKENTDGKSNDLSTAKRQHAILAELLLT
                     TEPLSLAHFMESYRISNTTFYADIKQLEESIRQLPLEIIRNQGYEIEGPEKYRRLLTA
                     NVLELEINEYELFHSISFDASLNYFFRFVDPQHLSLARKVIGEELIQKKTNLSDRKLE
                     HLVLMLTLTMERVTKNHLLTNETYNSLANKELLQTAKRLFSRIASETKQLYPVNEIVF
                     FAGLLNDFTNSFDQNFFEETFDTQLAYLVKQLIQEVSEETNVHFYEDETLYKMLLTHL
                     SGVFSRAVLQEETLTNPILEKIMVQYEEVANAIRKAVERIFQEKKLSEEEIAYMVLHF
                     ANSLERSPKESNISIAGFSPSGLASTSMLELRLKRYFPFIQQIHFYRIADLKKIDLQE
                     EYDVVISTSILPGYGGKYILVSPLLLEDEVKQLKEAFSHIEKKKRQKITYNSNNEWMI
                     EDTYEETMHFMDQINQLLGRFFIQELDNPETISETIRSLFPYFPEGLVTDEEVVHRRL
                     LKRYEQAPIGIPHTNMGLFHTSSSLISKPIFCIFNLKHPLTIEGMDRQPVDLSRMLVM
                     LAPSPIQEKDGILLGKISGAIIMNDLNTEIFRSGNKEIVYQLLAKILIEEIKK"
     gene            7196..7636
                     /locus_tag="IXT20_001773"
     CDS             7196..7636
                     /locus_tag="IXT20_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAQ4860212.1"
                     /translation="MIQLPAEKILLDQSYQNWKEALTEIGQWMTQQGGIKPAYIQSMI
                     ERQEKASVYIGNFVALPHGTDKDEEVVEEGIYLVQVPDGVDFGTKEEPKIATLLFVVA
                     LKKEHQLAVLQDLAFFCSDIDQVMALSDAETIEEVQMIFEHGHI"
     gene            complement(7836..8963)
                     /locus_tag="IXT20_001774"
     CDS             complement(7836..8963)
                     /locus_tag="IXT20_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ4860213.1"
                     /translation="MSLLTFERNLLLKNKVNFLFPLLLVVLFAFPLFFDHKLAYTEFD
                     ELNHNYEEMQRLIETLKEDENEKEFVESLEKSNKLIEAILHAKNTGNVQQMVEATYHY
                     EKDILDRLISGQRQGIPIIEQQKRVELLRYMKEHQIQRYSIFDLPAHLSLANYYENIF
                     SGMISSFLILCITALFLSSIISYEKRKQVISLVNLLPDSMVKKHSIRFTIYYGAAMLS
                     LVMPFLIVSILVIIKNGLGDFRYPVGTIIGQEIRILPMYEYLFQSFLFLLLWVLFLST
                     ISFLLSALFEHSLVNLLGTLLCLFLAEYRLFSSIGWIESISHYLPTSYVDFQNVIIGG
                     DIFSPLASEQVTFMNGILTLGIWSIVLLFIGMGTIYIKKSY"
     gene            complement(8966..9643)
                     /locus_tag="IXT20_001775"
     CDS             complement(8966..9643)
                     /locus_tag="IXT20_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ4860214.1"
                     /translation="MTLLIKNMNMKRGKKRVIRQLNLLVESGEVAALIAPNGFGKTTL
                     LNGIAQLLPTTYDQLSLHNISPTAYTDYRRSFFFLESTNQLSDYLSAVDYLTLIKYYW
                     QSKVECQTVFQRLKMTEYLTLPIGKMSLGMKQHVLLAAYLISDSSVMLFDEPLNGLDP
                     GSIDILNSLIRQWQKEGKTILLSSHAIANIQSICSKACFLKNGEIVTESSNMEEILAI
                     YRKLYEV"
     gene            9907..11673
                     /locus_tag="IXT20_001776"
     CDS             9907..11673
                     /locus_tag="IXT20_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HAQ4860215.1"
                     /translation="MERTIPKNNISLKARVQKLGSTLSSMVMPNIGALIAWGVLTALF
                     IPDGYLPNEAFATMVSPMLTYLIPLLIGYTGGKVIAGDRGSVVGAIATMGVIVGTDIP
                     MMLGAMIMGPLGGYAIKKFDQLFQKRIKSGFEMLVNNFSAGLIGFGLALLGFSAIGPV
                     VDALTQAMAKGVEIILSAHLIPLTSIFIEPAKILFLNNAINHGILTPLGTEQVAEAGK
                     SILFLLEANPGPGLGVLLAFTLFGKGAAKSSAPGAMIIHFLGGIHEIYFPYVMMKPLL
                     FLSVIGGGMSGSFIFQLMDAGLRAPASPGSIIAILAMTPFNSYLPVILGVIVAAAVSF
                     GISAVILKADAKDTNEDFEKSVQETKAAKQASKGAQGIPQGATFSGVQKIIFACDAGM
                     GSSAMGASILRKKVQEADLPQTVTNQAISQLTDDSNTLIVTQVELQERAKQKAPSARF
                     VSVENFLNSPRYDEIIQELSSKQKTSVSEIENEPAEEFLSANIKQIKEILLVHDDRSG
                     SATMGMTVLKDILDKNHLTIPLRKVNIQDLKDEPDSLIVAKESLAEKVQMAAPQSIHL
                     SVNSMVNPQKYESIAQYLKQTA"
     gene            11688..12122
                     /locus_tag="IXT20_001777"
     CDS             11688..12122
                     /locus_tag="IXT20_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAQ4860216.1"
                     /translation="MQSLPFDMIELNRSFKTKEEAIRYCGQMLVEAGCVDEEYIDAMV
                     DRDKMLSVYMGNFIAIPHGTDEAKKYVKKSGICVVQVPDGVDFGTEEEKIATVLFGIA
                     GVGDEHLQLVQQIALYCSDMDNVVQLADALSKEEVTENLAIA"
     gene            12135..13286
                     /locus_tag="IXT20_001778"
     CDS             12135..13286
                     /locus_tag="IXT20_001778"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HAQ4860217.1"
                     /translation="MRAVHFGAGNIGRGFIGEVLAANGFEITFVDVNHTIIDALNERK
                     EYEIELADESKEKITVKNVKGINNQTNPEKVIQEIAEADLVTTAIGPNILPFIAELIA
                     KGIQEREKEGSTTSLDIIACENMIGGSAFLEKEVYKYLPETSFTDKYIGFPNAAVDRI
                     VPLQHHEDPLFVQVEPFKEWVIDDSQRKNKKIQLKGVLYVDDLEPYIERKLFSVNTGH
                     ATVAYTGALLGYQTIDEAMQDALVVAQLKSVLQETGSLLIAKWGFDAEQHHAYIEKII
                     HRFQNKNISDAITRVARTPLRKLGYQERFTRPVRELQEHNLTCPHLTATMGIIFNYYD
                     PEDEQSRQLHEMKIHENLEQLIQEVTGINDPKTIGNIKQNVNRYAKQVA"
     gene            complement(13348..14046)
                     /locus_tag="IXT20_001779"
     CDS             complement(13348..14046)
                     /locus_tag="IXT20_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobyric acid synthase"
                     /protein_id="HAQ4860218.1"
                     /translation="MANYELNIAHLYGNLMNTYGDNGNLLMLKYIAEKMGVSCHTEIV
                     SIHEPFDADKYDLVFFGGGQDFEQLIISKDIQKKKDSLINYIENDGVMLAICGGYQLL
                     GHYYMGANGEKIQGISALDHYTLSQENNRFIGDIVIHNEEFNETYYGFENHNGRTFLG
                     EGERPLGKVVQGQGNNGEDQSEGVIYRNVFGSYFHGPILARNENLAARLIRLALEQRY
                     GKELDLPYLEGQTA"
     gene            complement(14039..15391)
                     /locus_tag="IXT20_001780"
     CDS             complement(14039..15391)
                     /locus_tag="IXT20_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HAQ4860219.1"
                     /translation="MGIRSHFAIAVGKTSQWALKTFFKGGSSLPGKLALKVDPHILDS
                     LAKDYQVVVVTGTNGKTLTTALTVNILRQQFDEVLTNPTGANMVQGIVSTFLQAKPKK
                     GQKKFAVLEIDEASLSKVTEYMKPELFLFTNIFRDQMDRYGEIYTTYKMIVDGAAKSP
                     NATIISNGDSPIFNSIETVNPRKYYGFDHEEDREQMAHYNTDGVLCPNCHHILHYKMI
                     TYANLGKYYCPNCDFKRPELDYRLTDMVRMDNVSADFVIDGHEYGIEVGGMYNVYNAL
                     AATAVAEYFNVDPDKIKQGLGYDEKVFGRQEVININGKKCTLVLVKNPVGINQVIDMI
                     GLAPYPFSLVALLNANYADGIDVSWIWDGNFENFADMDIPAVISGGDRHTDMTLRLKV
                     AGIPENKLTETKELPEVIEKIKELPTEHVYILATYTAVLQLRKELAAQGFIKGGMNRG
                     "
     gene            complement(15614..17014)
                     /locus_tag="IXT20_001781"
     CDS             complement(15614..17014)
                     /locus_tag="IXT20_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HAQ4860220.1"
                     /translation="MHVDLFFRRLQRRGQKFAANHFSSIQIIVFYYILMTVLSLVLFY
                     MPIFREPDSHVSFVDMLFMAISTVSVTGLSTFDINSVFNDNGIILLEILFQVGGLGIM
                     MISTAFVIFSKRRITLKQRQLIMTDMNQPRLSGIVRLIRITFAILIWFQLLFGTFFSI
                     YFYYRGYFDRWRDAVFYGFYQAISAVTNSGFDVTGDSIKPFAHDYFFLILIMFLIFVG
                     GIGFPVLMECREWLLYKRSNAKLPFRFSLFTKLAVLAFVILFVSGTVLIYLLEKDHLF
                     QDSNMSVKWINSMFYSITTRNAGLQIHDLGDFQITTLIIFSLLMFIGCSPSSVGGGIR
                     TTTVAIIGLYLYSFLKSEDNINIFGRRIDQDDVRKSVVVFMLSLGMCFFCIVFLSATE
                     EQTLISIIIEVTSAFGTTGLSLGITGDLSVVGKITIATLMFIGRIGMLYTLMLFVPKE
                     TRDLGYEYPSEKIIIG"
     gene            17175..17912
                     /locus_tag="IXT20_001782"
     CDS             17175..17912
                     /locus_tag="IXT20_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HAQ4860221.1"
                     /translation="MINAQQIVEKMKNQKINYDTVLKKMIMQWEKEEVKPKILIHTCC
                     APCSTHTLEFMSQYAEITLFFSNSNIHPKSEYLRRLHEQKRFVDQFNEKTDHHVALIV
                     DEYRPNEFTKMVLTNHLENEKEGGARCSACFNMRLDLVAQKAQELGYDYFGSALTISP
                     KKNAALINQIGMDIQKIYGTQYLPSDFKKNKGYERSLQMCKEYDIYRQCYCGCVFAAK
                     QQGCDLKEINREAKAYLKTSTVVFEEN"
     gene            18037..18516
                     /locus_tag="IXT20_001783"
     CDS             18037..18516
                     /locus_tag="IXT20_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A-agglutinin attachment subunit precursor"
                     /protein_id="HAQ4860222.1"
                     /translation="MRKVAPILKPIAVLTISTIVATTTGIVNSSSYRAPSSSSSTVEE
                     TTPTTSVSTSEPTTTESLNETTTTSTWDDTTTSSSYDETYTTDSSSETSNTVPDGTPS
                     DSTNPSTSPDTSASSETIPSSTTVDDNTQNDDAARTAQQNQDSGNTQTYNQQAGDSQ"
     gene            18513..19280
                     /locus_tag="IXT20_001784"
     CDS             18513..19280
                     /locus_tag="IXT20_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4860223.1"
                     /translation="MTFLLDLINGVHKFLGYLDISPKYLNRGYTILSVIPTLYLLRIV
                     YGLWQNQNYLQFFLYGLAFLVLVYFTVLNVFYYFLDKNTKADVTQLFVKYLPDEAFNI
                     QTEESIDNGNSIDKVNTQEVMVSYDEDYQLKLAENMRYLIGNGEIKTNELGRMDGFLV
                     DRNTLYPYYYVKKTGEKEYVLNIGRSYSDMEKVGTIRLDSTEDTLEPVGLFIVGGDFV
                     KDGLRYHEPYRLKLIAKKAQSQEEPVVYSRSQRRRHS"
     gene            19510..20079
                     /gene="lepB"
                     /locus_tag="IXT20_001785"
     CDS             19510..20079
                     /gene="lepB"
                     /locus_tag="IXT20_001785"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HAQ4860224.1"
                     /translation="MKEFLKNWGILILVLAAILLARVYVFTPVTVNGHSMDPTLSDGQ
                     RLISSKISNYERMDIITTKEPGDEERMIVKRIIGMPGDTVKMENDQLTINGKKYDEPY
                     LDEFKKEFSEDKLQGEYAYSSGFQAQAESSSTFTSDFEYTVPKGKYLVLGDNRLISKD
                     SRMFGLVDKDMIQGKVVFRYWPLSEIKIM"
BASE COUNT         6831 a         3604 c         4015 g         5674 t
ORIGIN      
        1 tgttcaaaag cttgcatctg cggtaataag cccaaccaaa cgaaaaatag gaggagctat
       61 ttttgtttgg ctgttcttat tacttgatgc agaacagctt tttcacaacc tctttttata
      121 agagttttta ctcattaatt tacaaaaaac aagcaaaatg cgaattttct cacatttttt
      181 ttaacaaaat caaagttatt gcttgaaaga agaagaatcc atcgttaaaa taaagcttga
      241 cgtagagggg agcaagaaat atgaaagatt ttcaattggg ggctgccacc tatttaaaaa
      301 gtgtagccat tcgggtgaaa gaccgagata aaatgattga tttttacaag aacgttgttg
      361 gctttgattt gaaaagagaa gaaaacgaat tagccatttt aggaacagaa gaagctggaa
      421 gtgaaatgtt gctgctggaa gaaagtccta gagcgaatga tcacatgggg gtgatcaaaa
      481 agctgaaccg tttttcattg attatcccta gcacagaaga aatggcagat atcctttgtc
      541 gtatccgtaa aatgaattat ccaatcaaag aagctttaga agatcatgga cgtatgggga
      601 ttttgctcgt agacccagaa gagaatgaat tggaaattta ccatgaagag aataagaacg
      661 accaaagaga aattccagaa acaatggatc aagaagcatt attaagaaaa tctgaagaaa
      721 aattcagtaa actatcaaca ggttcttatt ttgaaaaaat ccatttaaat gttactaact
      781 taacaaaaga acgccaattt cttaatcatg tacttggttt gaaggtacat aatgaagaag
      841 agggcatcca tgtattgaat gaaggagatt tccatgttgg tttgacagaa gctagcggag
      901 gcgcaattga tttgcctaca gatgaagtga ttggacttga ttttctgaaa ttcatgatcg
      961 atactgaaag catggaagga ctgattgctc acttgcagca actatcagtt gatttcttct
     1021 tagacaaaaa gcacacagta ctcacaatct atgatgctat aggaatcgaa tggtggttcg
     1081 tcagtgagcc aaaagaaaag aataaaactg acattcgatg cgacgcttaa ctttttcttt
     1141 cttaacgatt gtcgcatgtc tcgtcatttt atgaacggtc atactatcgc ttattctaga
     1201 gatagaaaga gaaaatagta taccggggga aactgcgcgg tttttcgcgc agtttttttg
     1261 tgtaaaaaag gttgattcct catagattaa gctttaagct tggactgtaa gaatctgata
     1321 gaaaagcagg agaaaaagat gtatccacac acgaaacagc ttattttgaa aaagatggaa
     1381 gaaggatttt atccaggtgt agcagtcgcc ttcataaaag aaaatcaagt agaaacattt
     1441 acagccggct ttgcccaagt cgttcctgaa aagcagctga tgacagaaga tctgttgttt
     1501 gatgcagcct cactgacgaa agtcgttgca accacttcat tagttttacg tctattagaa
     1561 gatggaaaga tagaactcga tcaaccgctt cataaatatc ttcctgaatt tgaaaatcag
     1621 acaatcactt tgcgtcattt actgacacat acttctgata tcaaaacatg gataccaaac
     1681 agagataagc tatctcagga agaattgatc gccgcttatt tgaagctaga accaggtgag
     1741 caactaggaa aagaagtaaa atatacagat gccggtacga ttcttcttgg gttgatgcta
     1801 gaggaaattt accaagaaac tgcagtggaa atctttcaag aacaagtatt ggaaccatta
     1861 ggaatgatga acagcttatt tcttcctttt ccttccaaaa aaatcgttcc cacagaacaa
     1921 ctgccttcgg gtgaggtttt gcgtggacaa actcatgatc cgaaagcaag agttttggct
     1981 gaacatgcag gtaacgcggg tctctttatt gatttgaaag acagcattac ttttgtccaa
     2041 atgtatttga acaagggaaa aatccgtaat ggtcgttttt tagaagaaga gacgattgat
     2101 tctttactgc aagatcaaac accgaaaaaa aatggctatc gttctttagg atgggactta
     2161 aaaaaggatg tagaagacgg tcatgcgatt ctttttcata ctggttatac aggtacgttt
     2221 ttcttgattg atataaaaac acaagaagcg ttgatatttt tgtcaaatcg cgtacatcct
     2281 gtcgatcatc gtgcggagta tctcatatgt cgagatgaaa ttgttgaaaa ctttttgaaa
     2341 gagaaagcgg tattaccgaa ataatgctat aatagctatg aaaaatgaga ggagtttgaa
     2401 ttatgcagga acctatgttt ttaaaaccag tttttcaaga aaaaatttgg ggaggaagtc
     2461 gtttacgttc agtctttggt tttgatatcc ctaacgataa aatcggagaa gattgggcga
     2521 tcagtgcgca tcctcatggt gtcagtgtag tagaaaatgg cgaattcaaa gggaaaagat
     2581 tagatgaact atggtcagaa cacaaggaat tgtttggcca tccttcagaa ccagtgtttc
     2641 cattattgat caagatctta gacgcagaag atgatctatc tgtacaagta catccagatg
     2701 atgcctatgg gatgaaacat gaaggtgaat taggaaaaac tgaatgctgg tatatcatcg
     2761 atgctgaacc tggtgcagaa atcatctatg gtcatcatgc aaagacaaga gaagaactag
     2821 ctgagatgat caaagatggg cgctgggatg accttttgaa gaaagtacca gttaaaaaag
     2881 gtgatttctt ttatgtacca agcggaacga tccatgctat tggtaaagga atcatgattt
     2941 tagaaacaca gcaaagttct gacacgactt accgcgtgta tgactacgac agaaaagatg
     3001 cgcaaggaca aacaagagag ctgcatatcc aacaatcgat cgatgtaaca actgtccctg
     3061 cgaaaacacc agaattacaa atcaaagaag tccgaaaagg gaattcatcg atcgtcactt
     3121 atttagaaac tgagtttttc aatgtatatg agtgggatat taaaggaatc acatctttca
     3181 aaaaacaagc cccttataca ttgatgacgg taatcgaagg agcaggagac cttgtcgttg
     3241 acggaaaaac atatccatta gaaaaaggaa caagctgtat tattccaaat ggcgtttctg
     3301 agtgggcggt ccaaggtgaa ctagcaatca ttgcgtctga accaggcaaa tagttcccag
     3361 atagctgaaa gcagcagacg gtttttcgtt tgctgctttt tctacataaa atccgtccat
     3421 caataaatag aagtctggtg aaaaagatgg aatacaaatg gaaagcaaac aacagcagtc
     3481 gcgtgtctat gaaacaactg agaaaagctg gacatcactt aaatgaaatg atcatgccaa
     3541 atctaagtct gtttatcgct tggggaatct tgtcattagc aacaaaaggt gtgtctggcg
     3601 atttgcacac cacattagaa gaggtatgcc attggatgat ccagctgctt ttacccatct
     3661 tgatcagtta ttcaggcggg aaatcggtag ggggacaaaa aggggcagtc gccggtgcaa
     3721 ttgcttcttt agggttgatc gctcgttcca atgctccaca aatcgtagga gcgatgatta
     3781 tcgggccgct tgccggcatc gtatatgaac aatttgtttc tagattccat gtgaaattca
     3841 aggctggata tgaaatgctt gtcagcaacc tactagtagg actgactggt agcgttattt
     3901 gtataattgg aatcgttttt attgaaccag ttttaaatag tgttcacttg ttgctcgcct
     3961 ccgttgtgtc ttggctagtc acaatgaatc tgttaccact tgtcagtctg atcttagaac
     4021 ctctaaaagt actatttttc aataatgcaa tcaatcatgg tgtattgaca cctttaggaa
     4081 tcgaattctc acaaacagtc gggtactctt ccttattttt gatggaggca aatcctggac
     4141 ctggtttagg gatcttactt tctatccttt gttttgcaga aaaacaggaa aaagtaaatg
     4201 cgagtggagc attgatgatc catgcgattg gcgggattca cgaaatctat tttccatttg
     4261 tcttgctgcg accgtatcta tttttagctg taatggctgg tggggcaagc ggcacgttga
     4321 tatttcaatg gttagatacg ggattagctg cacctgtatc gcctgggtca ctactcgtga
     4381 tttttgccaa tgcgccttat ccacaactag ctggggtaag tatagggata gcaatcagta
     4441 catttgtgac cttcatttgt gcagcgatac tattgaaatt accaaataaa gaaaaagaga
     4501 acaaaacggt caaagaaagg ggagaaatct tggaaccatc tatcattcat tccgtcattt
     4561 ttgcttgcga ctcaggtatg ggatcaagtg ctatgggagc cgccctgctg aaaaagaaat
     4621 tagcagcaga aaaaatcgat ctttctgtcg gatacagctc tatctatgaa ttgaataacc
     4681 aagtaaatca gctggttatc gtgcaacatg aactagcggg tcttgcaaga gaaaaagtac
     4741 ctcaaagtca aatctatgta ctggatcact ttttggaaac agatatcttg ctgtcacgaa
     4801 taaaagaagc gatccaattt ccaaagcggg aagaaaaaaa agagatagca gatatcacag
     4861 aacaaaaaac gtcgcctgct aatattgtat ttttgtataa aaacaacaga cgcggctcac
     4921 aaactatggg catgacgatt ttaagtcaat tagcaaaaaa agcgaaaaaa cagtttgtta
     4981 tctcaaaaga atcaatagaa aaaatcaaaa tccaagaaga aacgatttat atcgcacaaa
     5041 gagaactagc tgtaactgaa cagctagaag aaaagatccc tcggctagtt ctagttgaac
     5101 attgggtagc tacgaatgaa tacgaaaaac tcgtgaaggg agaaatataa gatgtattta
     5161 tcgcccagag aaaaacaatt attagcagaa tttctcagta gcccgtcacc tgtatctatc
     5221 caaaaaatga tgaatttatt gaaggtcagt aaacggacta tttaccgcga attagataat
     5281 cttgaagtct cattgaaatc ggtaaatgct tctttgaaaa aagcagctag aggctcattt
     5341 gtcattgagg catcagaaga agtattagag caattaaaag aaaatacaga tgggaaaagt
     5401 aatgacttat ccactgctaa acgacaacac gcgattttag ccgagttgtt actgacgaca
     5461 gagccgttga gtcttgctca tttcatggaa tcatatcgca tcagtaatac cactttttat
     5521 gcagatatca aacagttgga agaaagtatt cgccaactgc ctttggaaat cattcggaac
     5581 cagggatacg agatcgaagg accggaaaaa taccgccggc ttctgacagc gaatgtatta
     5641 gaattagaaa tcaatgaata tgaacttttt catagtattt ctttcgatgc atcactgaat
     5701 tatttttttc gattcgttga tccgcaacat ctatctcttg cacgcaaagt aatcggggaa
     5761 gaattgatcc aaaaaaagac caatctaagt gatagaaagc ttgaacattt ggttttgatg
     5821 ctgacattga cgatggagcg tgtgacgaaa aatcatttac tgacaaatga gacatataat
     5881 agtctggcaa acaaagaatt actacagaca gctaaacgat tgttttcacg gatcgcttct
     5941 gagacaaaac aactttatcc agtaaatgag atcgtctttt ttgcaggatt attaaatgac
     6001 ttcacgaact catttgatca gaatttcttt gaagagacgt tcgatacaca gcttgcttat
     6061 ttagtaaaac aactgattca agaagtaagt gaagagacga atgtccattt ttatgaagat
     6121 gaaactttgt acaaaatgct gttgactcat ttatctggtg tgttttcaag agccgtttta
     6181 caagaagaga cactgaccaa tccgatttta gaaaaaatca tggttcaata cgaggaagtt
     6241 gcgaatgcaa tccgtaaagc agtagagcgg atttttcaag aaaaaaaatt atccgaagaa
     6301 gagatcgctt atatggtcct tcattttgcg aattcattgg aacgaagtcc gaaagaaagc
     6361 aatatcagta ttgcaggatt ttcgccaagt ggattagctt ctactagcat gttggaacta
     6421 cgattgaaaa ggtattttcc cttcatccaa caaattcatt tctatcggat tgctgattta
     6481 aaaaaaatcg atttacaaga agaatatgat gttgttattt ctacatctat tttaccagga
     6541 tacggtggaa aatatatttt agtctccccg ctactgctag aagatgaagt caagcagcta
     6601 aaagaagctt tttcgcatat tgagaagaaa aaaaggcaaa aaattacgta taacagcaat
     6661 aacgagtgga tgatagaaga tacttatgag gaaacaatgc atttcatgga tcaaatcaat
     6721 caattactcg gtcgtttttt tatccaagaa ctggataatc ctgagacgat ttccgaaacg
     6781 attcgcagct tatttcctta ttttcctgaa ggactagtaa cggatgaaga ggtggttcat
     6841 cgccggctgc ttaagcgata tgaacaagca cctatcggta ttcctcatac aaatatgggg
     6901 ttattccata catccagctc attgatttct aaaccgatct tttgtatttt caatttgaaa
     6961 catccgttga ccattgaagg gatggataga cagccagtcg atttgtcccg tatgttagtt
     7021 atgctggcgc cttcgccaat ccaagaaaaa gacgggattc ttttaggaaa gatcagtgga
     7081 gcaatcatca tgaatgactt gaacaccgag atcttccgct caggaaataa agaaatcgtt
     7141 tatcagttgt tagcgaaaat cctgattgaa gaaataaaga aatgaaggtg aaaagatgat
     7201 acagctacca gcagaaaaaa tattgctcga tcaatcctat caaaattgga aagaagcgtt
     7261 gaccgagatc ggccagtgga tgacccaaca agggggaatc aaaccggcat atatccaatc
     7321 aatgatcgaa agacaagaaa aagcgagtgt ctatatcggg aatttcgtag ccttgccaca
     7381 tgggactgac aaagatgaag aagtagtaga agaaggaatc tatttagtcc aagtgcctga
     7441 tggtgtggac tttggaacaa aagaggaacc aaaaatcgcg acactcttat ttgtagtggc
     7501 tttaaaaaaa gaacatcagt tagcggtgct ccaagattta gcctttttct gttcagacat
     7561 cgaccaagta atggcgctca gtgatgcgga aaccattgaa gaagtccaaa tgatttttga
     7621 acatggacat atataaccaa tgttcacagt aaaaaaagct gccttgcatc aagtaataac
     7681 ttcaaccaag ataaaaataa gaggagctat ttttgttggg aggttcttat tactgcggat
     7741 gcaagctttt gaaaactatt tattctagaa aaccgtttat ttgaaatagg ggacggtgac
     7801 aaggtttttg tcaccgcccc tattattttt gtgttttaat agctcttttt tatatagata
     7861 gtgcccatac ctatgaataa taaaacgata ctccatatac caagtgtcaa gataccattc
     7921 atgaaagtaa cctgttctga agctagtggc gagaaaatat ctcctcctat gatcacattt
     7981 tgaaaatcaa cataagaagt tggcaagtaa tgactgattg attctatcca tccaatcgaa
     8041 gaaaacaagc gatactctgc taagaaaaga caaagaagtg tacctaaaag attgactaga
     8101 gaatgttcaa ataaagcaga tagaagaaaa ctgattgtac ttaaaaagag cacccacaac
     8161 aacaggaaca gaaagctctg gaaaagatac tcatacatcg gcaggattcg tatctcctga
     8221 ccaatgatcg ttcctacagg ataacggaag tctcccaaac catttttgat tattactagt
     8281 atagagacga tcaaaaatgg catcacgaga cttagcatag ctgcaccata ataaatggta
     8341 aaacggatgc tatgtttctt taccatacta tccggtaata gattgactag agagattacc
     8401 tgctttcttt tttcataaga gatgatggaa gaaagaaaca aggcggttat acataaaatc
     8461 aaaaaactag aaatcattcc tgaaaaaata ttttcgtagt aatttgctaa agagagatgc
     8521 gctggcaagt caaaaataga atatctttgg atctgatgtt ctttcatata acggagcaac
     8581 tcgacacgtt tttgttgttc aatgattgga attccttgtc tttgaccaga aatcagtcga
     8641 tccagtatat ctttttcata atgataagtt gcttcgacca tttgctggac attccctgtg
     8701 ttttttgcat gtaagattgc ttcgatcagt ttgttgcttt tctctaaact ttccacaaat
     8761 tctttctcat tctcatcttc tttcaacgtt tcaatcaaac gttgcatctc ttcatagtta
     8821 tgattcagtt catcgaattc tgtataagca agtttatgat caaaaaaaag tgggaatgca
     8881 aagagcacga ccaacaatag aggaaataaa aagtttactt tgttttttag taagagattg
     8941 cgttcaaatg taagtaaaga catagttaca cctcatataa tttacgataa atagctaaaa
     9001 tctcttccat atttgaggat tctgtgacga tttctccatt ttttagaaaa catgctttag
     9061 aacaaatcga ttggatattc gcgatagcgt gggaagaaag aagaatcgtc ttcccctctt
     9121 tctgccattg tctgatgagg ctattcaaga tatcaatact tcctgggtct aatccgttca
     9181 acggttcatc gaataacatg acagatgaat cactgatcaa gtaagcagca agcaagacat
     9241 gttgtttcat tcctagagac atttttccaa tcggcaaagt aaggtactca gtcatcttca
     9301 atcgttggaa aacagtttgg cattctactt tgctctgcca atagtatttg atcaaagtca
     9361 aataatcaac agccgataga tagtcggata actgattcgt tgattctaga aaaaagaaag
     9421 accggcgata atctgtataa gcagtaggag agatgttgtg aagggacaat tgatcataag
     9481 ttgttggcag cagttgggca atgccattca gcaaagttgt tttgccaaat ccgttcgggg
     9541 caatcaaagc agctacttct cctgattcta ctaaaagatt caattgtcga atcacacgtt
     9601 ttttaccacg tttcatgttc atgtttttga tcagtaatgt catagatagg cctccaatat
     9661 aaaatttaat aacataatag aatggaaaac atctaaatta aatgctaaca aaagttattg
     9721 tttgcatatt ataaaaaaat taaaataaca gttttattta aaattgaagt tttctgatac
     9781 cgaaatcttt ttattttgct gacatactca aacctttggc acaaagtttc gtgccaaagt
     9841 acgactattt ctctttttag taagcggata caattagagt atcaaaagga gagagggaga
     9901 gacaaaatgg aacggacgat tcccaaaaac aacatttcgc taaaagcgag ggtacaaaaa
     9961 ttaggcagta ctttatcaag catggtcatg ccaaatattg gagcactgat tgcctggggt
    10021 gtattaacgg ctctatttat cccagatggt tatttaccaa atgaggcttt cgctaccatg
    10081 gtaagcccaa tgctaactta tctgattcct ttattgatcg gttatactgg cggaaaagta
    10141 attgctggtg accgcggatc agttgttggg gcaattgcaa cgatgggtgt gattgtcgga
    10201 acagatattc caatgatgct aggcgcgatg atcatgggac ctcttggtgg atatgccatc
    10261 aagaaatttg atcagctatt ccaaaaacgg atcaaatccg gttttgaaat gctagtcaat
    10321 aatttttcag caggtcttat cggttttgga ttagcgttat taggtttttc tgcgattggt
    10381 ccagttgtcg atgcattgac tcaagcaatg gcaaaaggtg tagagatcat tttatctgcc
    10441 catttgattc cattgacaag tatttttatt gaaccagcaa aaatcttatt tttgaataat
    10501 gcaatcaatc atgggatttt gacaccattg ggaactgaac aagtagcaga ggctggaaaa
    10561 tctattctat tcttgcttga agcaaatcct ggtccaggac ttggcgtatt acttgctttt
    10621 actttgtttg gcaaaggagc agcaaaatct tcagcaccag gagccatgat cattcacttt
    10681 ttaggcggga tccatgaaat ctattttcct tacgttatga tgaaaccgtt attattctta
    10741 tcagttatag gcggaggaat gtcaggaagt ttcattttcc aattgatgga tgctggacta
    10801 agagcgccag cttctccagg gtcgatcatt gcgattctag cgatgacacc gttcaattct
    10861 taccttcctg ttattttggg cgtgattgtt gccgcagctg tttcgtttgg tatttctgca
    10921 gtaattttga aagcggatgc gaaagataca aatgaagact tcgaaaaatc tgttcaagag
    10981 acaaaggcag ctaagcaagc ttccaaaggt gcccaaggta ttcctcaagg agctacattc
    11041 tctggcgtac aaaaaatcat ttttgcttgt gatgcaggaa tgggatcaag tgcgatgggc
    11101 gcttcgatct tgagaaaaaa agtacaggaa gcagacttgc ctcaaacggt gaccaatcaa
    11161 gcaatcagcc aactgacaga tgactcgaat acattgatcg ttactcaggt cgaactacaa
    11221 gaaagagcaa aacaaaaagc accgagcgcg cgttttgtct cagtagaaaa ctttttgaat
    11281 tcacctcgat atgatgaaat cattcaagaa ttaagcagca agcagaaaac atcagtttct
    11341 gaaattgaga atgagccagc agaagagttt ctttcagcaa acatcaaaca aatcaaagag
    11401 attctacttg tccacgatga tcgttccgga tcagcaacaa tggggatgac cgtgctcaaa
    11461 gatatattag acaagaacca tttaactatc ccgttaagaa aagtcaatat ccaagattta
    11521 aaagatgaac ctgattcact gatcgtagcc aaagaaagtt tagcggaaaa agtacagatg
    11581 gctgcacctc aatccattca tttatcagtc aacagcatgg tcaatccgca aaaatacgaa
    11641 tcgattgcac aatatttgaa acagacagct taagggagga aaacagcatg caaagtttgc
    11701 cattcgacat gatcgagctc aatcgatctt ttaaaaccaa agaagaagcg atccgctatt
    11761 gcggtcagat gcttgtagaa gcagggtgcg ttgatgagga atatatcgat gccatggtag
    11821 atagagacaa aatgttatct gtttatatgg ggaattttat cgcgattcct catggaacag
    11881 atgaagcaaa aaaatatgtg aaaaaatccg gcatctgtgt cgttcaagta ccagatggtg
    11941 tagattttgg taccgaagaa gaaaagattg cgactgtttt gttcggtatt gcaggagtcg
    12001 gagatgagca tttgcaactt gtacagcaaa tcgcacttta ttgcagtgat atggacaatg
    12061 ttgtccagct agcagacgcg ctcagcaaag aagaagtaac ggaaaatcta gcaattgctt
    12121 aaggagagag aaaaatgaga gcagtacatt ttggtgcagg aaatatcggt cgaggattta
    12181 ttggagaggt tttggcagct aatggatttg agattacttt cgtagatgtg aatcacacga
    12241 tcattgatgc tttaaatgaa agaaaagaat acgaaatcga attagcagat gaatcaaaag
    12301 aaaaaatcac tgtaaaaaat gtcaaaggaa tcaacaatca aacaaatcca gaaaaagtaa
    12361 tccaagaaat cgctgaagca gatttagtta ctaccgccat tggcccgaat atcttacctt
    12421 ttattgctga gttgattgca aaaggtatcc aagaaagaga aaaggaagga agcacaacat
    12481 ctttagatat cattgcctgt gaaaatatga ttggcggttc agcatttcta gaaaaagaag
    12541 tgtataaata tcttccggaa acatcattta ctgacaaata tatcggcttt ccaaatgcag
    12601 cagtcgatcg aatcgtccca ttacaacacc atgaagatcc tttattcgtc caagtggaac
    12661 ctttcaaaga atgggtcatt gacgatagcc aaagaaaaaa taaaaaaatc caattaaaag
    12721 gggttttata tgtagatgat ctagaaccat atatcgaacg caaactattt agtgtcaata
    12781 ctggtcatgc gactgtcgct tatacaggtg ccttgttagg ttaccagacc attgatgaag
    12841 caatgcaaga tgcgttagtt gtggcacaat taaaatcagt tttgcaggaa acaggaagtt
    12901 tgctgattgc caaatggggg tttgatgcag agcagcatca tgcgtatatt gaaaaaatca
    12961 ttcatcgctt ccaaaacaaa aatatctctg atgcgatcac acgtgtcgct cggacaccat
    13021 tacgtaagtt aggttatcaa gaaagattta cacgtccagt gagagaacta caagaacata
    13081 acttgacttg cccacatttg acggcaacaa tggggattat ttttaattac tatgatcctg
    13141 aagatgaaca aagccgtcaa ttgcatgaaa tgaagatcca tgaaaacttg gaacaattga
    13201 ttcaagaagt cacaggaatc aatgatccga aaacaatcgg gaatatcaag caaaacgtga
    13261 accgttatgc caagcaagtc gcttaaaata ggatacgaaa aaggggaatg tgacgaaatt
    13321 tttgtcacat tccccttttt cgaataacta ggctgtttgt ccttcaagat agggcagatc
    13381 caattcttta ccgtaacgtt gttctaaagc taggcggatc aaacgagctg ccaggttctc
    13441 atttcttgca aggataggtc catggaaata agaaccgaag acatttcggt aaatgacacc
    13501 ttcagattgg tcttcaccat tatttccttg accttgtacc actttcccca aaggtcgttc
    13561 accttcacct aagaaagtcc gaccattgtg gttttcaaat ccataataag tttcattgaa
    13621 ttcttcgtta tggataacga tatcaccgat aaaccggtta ttttcctgac ttaacgtgta
    13681 gtgatccagt gcactgattc cttggatctt ttctccatta gctcccatgt agtagtgacc
    13741 tagcaattgg tagccaccac aaatggctaa catgacgcca tcattttcga tatagttgat
    13801 caaagaatct ttctttttct ggatgtcttt tgaaatgatt aattgctcaa agtcttgacc
    13861 accgccaaag aaaaccaagt catatttatc agcatcaaaa ggttcatgta tactaacgat
    13921 ctccgtatgg caagaaaccc ccattttttc tgctatatat ttcaacatca aaaggtttcc
    13981 gttatcacca tatgtgttca tcaaatttcc ataaaggtgg gcgatattga gttcataatt
    14041 agccacggtt cattcctcct ttgataaatc cttgagcagc tagttcttta cgaagctgca
    14101 atacagcagt gtaagtagct aaaatataga catgctcagt tggcaattct ttgatttttt
    14161 cgattacttc tggtaattct tttgtttctg tcaatttatt ttctggaatc ccggcaactt
    14221 ttagacgtaa agtcatatct gtatgacggt ctcctccaga aataacagct gggatatcca
    14281 tatcggcaaa gttttcaaaa ttaccatccc aaatccagct gacatcgatt ccgtctgcgt
    14341 aattggcatt taataaagcc actaaagaaa atggataagg agctagacca atcatatcga
    14401 ttacttgatt gatacctaca ggatttttga caagtactaa tgtacatttt ttcccattga
    14461 tattgatgac ttcttgtcgg ccgaatactt tctcgtcata tcctaaacct tgtttgattt
    14521 tatcaggatc gacattgaag tactctgcca ctgcagtagc tgctaaagca ttgtagacat
    14581 tatacatacc gccaacttcg attccgtatt catgaccatc gatcacgaaa tctgctgaca
    14641 cattgtccat ccgtaccata tcagttaatc gataatccaa ttcaggacgt ttgaagtcac
    14701 aatttggaca gtaatatttc cctaagtttg cataagtgat cattttataa tgcaagatgt
    14761 gatgacaatt tggacataag acaccgtctg tattgtaatg agccatttgt tcacgatctt
    14821 cttcatggtc aaagccataa tattttctag gattgactgt ttcgatagaa ttgaagattg
    14881 gtgaatcacc gttactgata atcgttgcat ttggtgactt tgcggcaccg tctacaatca
    14941 ttttataggt cgtatagatt tctccatacc gatccatttg atcacggaaa atatttgtaa
    15001 ataagaacag ttctggtttc atatattctg tgactttgct caaacttgct tcatctattt
    15061 ctaggacagc aaactttttc tgtccttttt ttggttttgc ctgcaagaaa gtcgatacaa
    15121 ttccctgtac catattcgca ccagtagggt tggttagaac ttcatcgaat tgctggcgca
    15181 aaatattgac tgttaaagcg gtagtcaatg ttttaccgtt tgttccggtg acaacgacga
    15241 cttggtagtc ttttgccaaa ctatctagta tatgtggatc gactttcagc gctagtttac
    15301 ctggtaaact tgatccgcct ttaaaaaaag tttttaatgc ccattgcgac gtttttccaa
    15361 ctgcaatcgc aaaatgactg cgtattccca ttatgtaacc tccaaaaacg atttgctgac
    15421 gaataaattc aactaatcat accatatgtt gaaaaaaagg ttaaattgct tttcttattc
    15481 ttaagaaaag gttacggctg aaagtgcatg aaatcggaca aaatcgcatg atttaacaaa
    15541 aaaacagctt gaaacaaaat gattgtttca agctgctgtg attttattat tccctcttac
    15601 ctatattccc attttaaccg ataataattt tctcgcttgg atattcatag cctagatctc
    15661 gtgtttcttt tggcacgaat aacatcaatg tatatagcat tccgatccgc ccaataaaca
    15721 tcagtgtagc aatcgtgatt ttgcccacaa cagacaagtc tccggtaatc cctaaagata
    15781 aaccagttgt tccaaacgca gaagttactt cgatgatgat cgagatcagc gtttgttctt
    15841 ctgtagcaga taaaaaaacg atacaaaaga aacacatacc aagagaaagc ataaagacaa
    15901 cgactgattt tcgcacatca tcttgatcga ttctccgtcc aaaaatattg atgttatctt
    15961 cacttttgag gaaggaatat agatacaaac cgatgattgc tacagtagtt gttcgaatcc
    16021 cgccgcctac agaactagga ctgcatccga taaacatcag taaagaaaaa atgatcagtg
    16081 ttgtgatttg aaaatcaccc aaatcgtgaa tctgcagacc agcattacga gtcgtaatcg
    16141 aataaaacat cgaattgatc catttgacac tcatattgga atcttggaat aaatggtcct
    16201 tttccaatag ataaatcaag acagttcccg aaacaaacaa gataacaaaa gctaacacag
    16261 ctaatttggt aaacaaggaa aaacgaaacg gaagcttcgc attggaacgt ttgtataata
    16321 gccattcccg gcactccatc aatacaggaa aaccaattcc tccgacaaaa atcaaaaaca
    16381 tgatcaggat caagaaaaaa tagtcatgtg caaagggctt gatggaatcc ccagtgacgt
    16441 caaacccaga gttcgtgaca gccgatatcg cctgataaaa accgtaaaaa acggcatcac
    16501 gccaacgatc aaaatagcct ctgtaataaa aataaataga aaagaatgtc ccaaaaagta
    16561 attgaaacca gattaaaata gcaaaagtga tccgaatcag tcggacgata ccagacaaac
    16621 gcggttgatt catatccgtc atgatcagct gtcgctgttt cagtgtgatc cgtcgtttag
    16681 aaaaaatgac aaaagcagta gaaatcatca tgatccccaa accaccaact tgaaataata
    16741 tttccagcag tataataccg ttatcattga acacagaatt gatatcaaaa gtcgatagtc
    16801 cagtcacact cactgtactg attgccatga acagcatgtc cacaaaagaa acatggctat
    16861 ctggttcgcg aaaaatcggc atataaaata gaacgagtga taagaccgtc atcaatatat
    16921 aataaaaaac gatgatttgg atcgatgaga aatgattggc agcaaacttc tgtcctcgcc
    16981 gctgcagcct tcgaaagaat aaatcaacat gcacagtact atcctcctga caatagtcgt
    17041 ttctaccagt atacaagggg aattaggaat actcaaagaa attatcaaac agataacaat
    17101 acagctttcg attaaaatag aaatggctta taataaggaa aatcctagaa aaaagaagga
    17161 ggaggaataa caaattgatt aatgctcaac aaatcgtaga aaaaatgaaa aatcaaaaaa
    17221 taaattatga tacagttctg aaaaaaatga tcatgcaatg ggagaaagaa gaagtaaagc
    17281 ctaagatatt aatacataca tgctgtgctc cttgcagtac gcacacattg gaattcatgt
    17341 ctcaatatgc agagatcacg ttattttttt caaattcgaa tattcatcct aaaagtgagt
    17401 atttgcgaag attacatgaa caaaaacgat ttgtagacca atttaatgag aaaactgacc
    17461 atcatgttgc gctaatcgtt gatgaatacc gtccaaatga attcaccaaa atggttttaa
    17521 caaaccatct tgaaaacgaa aaagaaggtg gtgctcgctg cagtgcatgc ttcaatatgc
    17581 gattagatct tgttgcgcaa aaagcgcaag aactaggata cgattacttt ggaagtgccc
    17641 tgaccatctc gccaaagaaa aatgcagctt tgatcaatca gatcggaatg gatatccaaa
    17701 agatttacgg gacacaatac ttgccaagtg atttcaagaa aaacaaaggc tatgaacgct
    17761 ctttgcaaat gtgcaaagag tatgatattt atcgccaatg ttattgtggt tgcgtgttcg
    17821 ctgccaagca gcaaggatgc gatctgaaag aaatcaatcg ggaagcaaaa gcttacttaa
    17881 aaacatcaac cgtagttttt gaggaaaatt aaagaaatct ttcggaaaaa cgtaagaaaa
    17941 aaataaagaa tttatggtag aaataaggta gactgtataa tttgtccgta gtaaagtaag
    18001 gaaacaagaa taaatttcaa tgaaggaaga gataagatgc gaaaagtagc acctattttg
    18061 aagccaatcg ctgtattaac aatctcgacg attgtcgcga ctactacagg tattgtgaat
    18121 agttccagct accgtgcacc aagttcaagt tctagtactg tagaagaaac aacaccaact
    18181 acttcagtct cgacatctga accaacaaca acggaaagtt tgaatgaaac cactacgaca
    18241 agtacatggg acgatacaac aactagttcg tcgtatgatg agacatatac aactgacagc
    18301 tcaagcgaaa caagtaatac ggtaccagat ggcactccat cggattcaac caatccaagt
    18361 acaagtccgg atacaagtgc tagctccgaa acgataccaa gcagtacgac tgtagatgat
    18421 aacacgcaaa atgatgatgc agcacgtaca gcacagcaaa accaagattc aggaaatacc
    18481 caaacgtata accagcaagc aggtgattct caatgacatt tttactagac cttatcaacg
    18541 gggtgcataa attccttggc tatttagaca tcagtcctaa atatcttaac cgtggatata
    18601 cgattttaag tgtgattccc acgctttatt tattgagaat tgtttatgga ctttggcaaa
    18661 accaaaacta tcttcaattt ttcttatatg gacttgcatt tctagtgctt gtttatttta
    18721 ctgttttaaa tgtattttat tactttttag ataaaaacac gaaagccgat gtcactcagc
    18781 tttttgtgaa gtatttacct gatgaagcgt tcaacatcca gacagaagaa tcgatagata
    18841 atggaaattc gatcgataaa gtgaatacac aagaggtcat ggtttcttat gacgaagatt
    18901 atcaattgaa attagccgag aatatgcggt atttgattgg aaatggtgag atcaaaacaa
    18961 atgaactcgg tcgcatggat ggatttctgg tcgatagaaa tacattgtat ccttattact
    19021 atgtcaagaa aacaggagag aaagagtatg ttctaaatat tggacgaagc tactcggaca
    19081 tggaaaaagt agggaccatt cgacttgact caacagaaga cacattggaa cctgtcggat
    19141 tgttcatcgt cggcggtgat tttgtcaaag acgggttacg ctatcatgag ccttatcgtc
    19201 tgaaattgat tgcaaaaaag gcacagtcgc aggaagaacc agtggtttat agtcgtagcc
    19261 aaagaagaag acattcataa tgtgatgaaa cgccgtgtat cagtggttgc gataaaaacg
    19321 tttattcgga ataaggggga tgtgacacgt cttttgtcac gttccttttt tatataacga
    19381 gaaagataag acgaacacat cacttattct ttcctttcat ttataaacat tgtagaataa
    19441 agaaagtaca acgaagtatt gtgaaaaaac gtaataaata acgaggaaaa tacaagtagg
    19501 aggtaagctg tgaaagaatt tttgaaaaat tgggggattt tgatccttgt attagcggct
    19561 attttattag ctagagtgta cgtgtttaca ccagttactg taaacgggca ttcgatggac
    19621 cctacattaa gtgatggaca acgattgatt tcatctaaga tctcaaacta tgaacgaatg
    19681 gatattatta ctacaaagga accgggggat gaagaacgga tgattgttaa acggatcatc
    19741 ggtatgcctg gagatacagt caagatggaa aatgaccagt tgaccattaa tggcaaaaag
    19801 tatgatgaac cttatctgga tgagttcaaa aaagaatttt cagaagataa gttacaagga
    19861 gaatatgctt atagctcagg attccaagca caagcagaaa gttcatccac ctttacgagt
    19921 gactttgaat atactgtgcc aaaaggaaag tatcttgttc taggcgataa ccgcttgatt
    19981 tcaaaagaca gccgaatgtt tgggttagtc gacaaagata tgatccaagg aaaagttgtt
    20041 ttccgttatt ggcctctttc agaaattaaa atcatgtgat gaaaaaataa aaagaagctg
    20101 tgacatgttt tttgtcacag cttc
//
LOCUS       DACGLE010000045        20009 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0534
            SAMEA3304449-rid9560453.denovo.049, whole genome shotgun sequence.
ACCESSION   DACGLE010000045 DACGLE010000000
VERSION     DACGLE010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304449
            Sequence Read Archive: ERR985064
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20009)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20009)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702225.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702225.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 20:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,045
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 27 of 67
            Pseudo Genes (incomplete)         :: 25 of 67
            Pseudo Genes (internal stop)      :: 19 of 67
            Pseudo Genes (multiple problems)  :: 4 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20009
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304449-rid9560453.denovo.049"
                     /strain="VREN0534"
                     /db_xref="taxon:1352"
     gene            complement(198..1181)
                     /locus_tag="IXT20_001786"
     CDS             complement(198..1181)
                     /locus_tag="IXT20_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
                     /protein_id="HAQ4860225.1"
                     /translation="MRAISIKHPGSVDVLEQIEVPVPKPKQGELLIKVHTAAVNRTDI
                     MKRESVSLKPPYPILGVEVAGEVVENKSDISTFTPGTKVYGLVNLGGYAEYAVMPADR
                     AILLPDTLDYVSAAGISEVFLTAYQTLYWLGKLQEGETVLIHAGASGVGTAAIQLARQ
                     LSHAKIIVTAGSEEKLAFCKELGADELINYKKQDFQEEVRKITEGRGVDVILDFIGAS
                     YWEKNLASIAVDGRWVLIGMLGGTIVPEIDLGVLIGKRVQLLGTLLTPRSDQYKAELA
                     QTFMQKAGPFLENGKIRPIIDRTFQFSAVKQAHEYMEANKNIGKIILKITE"
     gene            complement(1186..1833)
                     /locus_tag="IXT20_001787"
     CDS             complement(1186..1833)
                     /locus_tag="IXT20_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAQ4860226.1"
                     /translation="MKIFVVGANGQIGRHLIKDLAPSSHEIFAGVRDVASQTLVKKEN
                     VSYVSFDLTWSVEKMTEAFKGIDVLIFAAGSQGKNLLQVDLDGAIKTVIAAENAHVSR
                     YLMVSAVYADEPAKWPESMTDYYITKHYADEWLKRTNLDFVILQPVTLTNDEEVTSIQ
                     LTKPNEKASKTITRSTVAAVLAALVEETDISRTTLVLSEGSKELNTAFQEWAKEE"
     gene            complement(1918..2550)
                     /locus_tag="IXT20_001788"
     CDS             complement(1918..2550)
                     /locus_tag="IXT20_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAQ4860227.1"
                     /translation="MKNTKNFTLTAMFLAILILLAVTPLGFIPIGPINATTMHIPVII
                     ASIVLGPRLGAFLGGTFGLISMIRSTVIQTPLSFVFSPFIPVIGTEHGSWKALLIAFI
                     PRILIGVVPYFVYKGLRKLMKQKADSVSLFLAGLSGSLTNTILVMNMIYFLFQQDYAK
                     VIGTNLNAVYSAILAVIFTSGIPEGIVAGLATAAVCNVLLRYFKHPSLTN"
     gene            complement(2566..3117)
                     /gene="coaC"
                     /locus_tag="IXT20_001789"
     CDS             complement(2566..3117)
                     /gene="coaC"
                     /locus_tag="IXT20_001789"
                     /EC_number="4.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HAQ4860228.1"
                     /translation="MKKILLGVTGSISAYKSADLTSQFVKLGAQVDVIMSKSSTHFIT
                     PLTLQSLSKRAVHTDVMQESNPQVINHIELAKQADLFLIAPATANIIGKLANGIADDL
                     LSTVAMALPVGTPKLIAPAMNTNMYQNPITQRNLETLRSVGYQEIEPRESLLACGDFG
                     KGALADIQTIIEQVMLTLNERNA"
     gene            complement(3114..3851)
                     /locus_tag="IXT20_001790"
     CDS             complement(3114..3851)
                     /locus_tag="IXT20_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014386989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenate--cysteine ligase"
                     /protein_id="HAQ4860229.1"
                     /translation="MKILITAGGTTEKIDQVRAITNHSTGRLGQALADYLAANPDTTI
                     DYVTTKQALKPKRHSNITIYTIESALDLFLQLEELTKKEHYDAIIHSMAVSDFTPAFS
                     FSEEQLAKNLPTSSTQEELDNWFAENEQTDTTVSKISSNTEHLVLVLKKTPKIISYLR
                     EWQPKAKIIGFKLLVDVPKESLLAVAKNSLINNKTDFIFANDLTEIHGETHHGYLLSK
                     DGTVEEAQSKSEIAALITEKIRLEESK"
     gene            complement(3848..4837)
                     /locus_tag="IXT20_001791"
     CDS             complement(3848..4837)
                     /locus_tag="IXT20_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HAQ4860230.1"
                     /translation="MNAFRSFQVIKEPVFHTAISDLPFTELAENEVLVKIAYSDVNYK
                     DALAMSESGQVIRTYPMTPGIDLSGTVVQSNNPRFSKEDLVLATGFGLGVTHPGGYSQ
                     YQKVPGDWLVPLPKNMTLRQAMILGTAGFTAMLCVNALIRQGMTSDKKVVVTGASGGV
                     GSTAIALLHKLGFQSIIAFSRKEESVTWLKSLGALQVVRPDEFLPETTKALGKQQIDY
                     VIDTVGGEQLSSLLPLISYNGAVALCGNAGGIKLNATVLPFILRNIQLIGIDSVNVPI
                     DQRLSLWQQMADLQIADELVVQEITLDQLPETASKLLAGTHQGRTLVNVGDHK"
     gene            5095..5637
                     /locus_tag="IXT20_001792"
     CDS             5095..5637
                     /locus_tag="IXT20_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ4860231.1"
                     /translation="MEIGEKLRNLRIQKNLTQEELGERTDLSKGYISQLERDLSSPSM
                     ETFFSILEVLGVTPEEFFHQEAANLQVVYSKEDHTVYFDEENGYELEWLVADSNEKEM
                     EPVLLTFEGAGEYKTFEPSLSETFIFVLEGELELSLGETVYVAKAGQSIYYQATKQHQ
                     LRNHLKKRTKVLIVATQSYL"
     gene            5654..6739
                     /locus_tag="IXT20_001793"
     CDS             5654..6739
                     /locus_tag="IXT20_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016182253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ4860232.1"
                     /translation="MGKPIISFDQVVKRYDDETVLKKVSFEIEQGKFYTLLGPSGCGK
                     TTILRIIAGFTEATEGDIYFEGKRLNDLPANKRQVNTVFQDYALFPHMNVFDNVAFGL
                     KIKKMSKSDIEKKVKDALRMVQLPGYEKREISEMSGGQRQRVAIARAIVNEPKVLLLD
                     EPLSALDLKLRTDMQYELRDLQQRLGITFIFVTHDQEEALAMSDEIFVMNKGKIVQSG
                     TPVDIYDEPINHFVADFVGESNIVDGVMIEDNLVEFVGKQFECVDGGMRLNEPVEVVL
                     RPEDLTITTPDKGKLVVTVDTQLFRGVHYEIICYDEQQNEWMVHSTKKAKEGSKVGLA
                     FEPEDIHVMRFNESEEEFDARLDSYEE"
     gene            6751..7557
                     /locus_tag="IXT20_001794"
     CDS             6751..7557
                     /locus_tag="IXT20_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ4860233.1"
                     /translation="MKTMRRIYSVPYILWLGLFVIAPVLMIIYQSFFDMNGQFTLNNY
                     LSYFTSGTSLSMTINSVWYAFLITFFTLIISYPTAYFLTKLKHKQLWLMLIILPTWVN
                     LLLKAYAFIGIFSVHGSVNHFFDFIGIGPKQILFTDFSFLFVATYIEIPFMIMPIFNA
                     LEEMNPSLISASRDLGANSFETFRRVIFPLSLNGVKSGVQAVFIPSLSLFMLTRLIGG
                     NRVITLGTAIEQHFLVTQNWGMGSTIGVILIIAMFIVMLLTGEKKKGGRS"
     gene            7558..8385
                     /locus_tag="IXT20_001795"
     CDS             7558..8385
                     /locus_tag="IXT20_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ4860234.1"
                     /translation="MKKFKWSYLYLGIVFLFLYAPIFYLIFYSFNEGGTMNSFTGFTW
                     ENYEAVFEDTRLIIIVLNTFMLAFLSALIATIIGTFGAMGIYYTKKRRARTALLSMNN
                     ILLVSPDVIIGASFLIFFTLLGFGLGFQSVLLSHIAFSIPIVVLMVLPKLQEMNDSMV
                     DAARDLGANNFQVVKNIILPYLSPGIIAGYFMAFTYSLDDFAVTFFVTGNGFTTLSVE
                     IYSRARQGISLEINALSALVFLFSMILVIGYYFISKENRPKRLKKNRRVREGVARLR"
     gene            8382..9455
                     /locus_tag="IXT20_001796"
     CDS             8382..9455
                     /locus_tag="IXT20_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAQ4860235.1"
                     /translation="MKKLQSLFLGILIIIAVLLLGVRQLEKTSGMSGAKVLTIYNWGD
                     YIDPSLITKFEKEYGYKVNYETFDSNEAMFTKIQQGGTNYDISIPSEYMIQKMIKEKL
                     VLPLDHSKIKGLNNIDPRFLDLDFDPDNQYSIPYFWGTLGIVYNDKFFDANQIKHWDD
                     LWKPELKDSLMLIDGAREVMGLSLNSLGYSLNSKNMAQLTEAANKLNHLTPNVKAIVA
                     DEIKMYMINEEASVAVTFSGEAADMMWENEHLHYVIPPEGSNLWFDNIVMPKTVKNKE
                     GAYDFINFMLEPENAAQNAEYIGYSTPNEEAKALLPKDISSDEQFYPSDDTISHLEVY
                     KDLGSKYLGIYNDLFLEFKMYRR"
     gene            9883..12579
                     /locus_tag="IXT20_001797"
     CDS             9883..12579
                     /locus_tag="IXT20_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ4860236.1"
                     /translation="MEYKYRIDILQPIVYYAFRHIKLEEKMKKSAKDRVFDELIRLTG
                     NMSSVTAEKISKNLGISRQNASHYLTRLVEDKKVEKLPGKPVLWKPLDEYAVVDNTEQ
                     INEAFHSVVGHDGSLREVIQKCIAAVKYPPNGLSVLINGATGVGKSFLATKIFEYAIH
                     EQIIEKDAPFAILNCADYADNPELLSATLFGYKKGAFTGAEKDTDGLLATANNGYLFL
                     DEVHRLSKENQEKLFLFIDTGNYRPVGENVNWHSAKVRFIFATTEKGENYLLDTFDRR
                     IQISVMLPTFEDRPIRERLELIQLFFQNEADVLQKDIIVSREALSLMLSHPFSGNIGK
                     LKNIIKISCADVYSRTQEEPLTIGKNEILIQLNMLESEVESLPIASLLIKKNQKIKTD
                     RIKPFDYDSILEKITQLINEGNFNENFSSIKNEVQHLVHHIDKDSFLSMSRKLMIQMF
                     RKVWVSVLGKTYGLTTSGPVAEIISRSYVISPSYKTDLTGINRTLAANLPRTTYLCNQ
                     FLEHLPPLSEKDENFLRLLIVVTLSDYVDESIELKGLLLAHGDSTASSIQAVVNQLCG
                     NYVFEALDMPVESNVSEIVESTRNFVKRQVNTDSLVLIVDMGSLNQIYTQIKNDLKGE
                     LLLLNNLTTSIALDIGLKMTMNAPFKQIAEDAADKYSINVQYFEGFSQSSNIIISCMS
                     GLGISDKLKDVFRHHLSDESIEVFTKDYRELRELIDQNDETYFDKTKLVITTSDLPRS
                     FSIPNINVYDILDGDGAANLEHILSPEMKPAEFNRLIQELVKFFSLEGIADRLNFLNP
                     TIVMSEVETVISKYENYYHFTLNGRVKLNLYMHTALMMERLFLARTEKSEEPNYDLKP
                     DEDEFYYVSRSIFQSMEMKYNFKVNAYELSLLYELLGPYIQK"
     gene            12655..13143
                     /locus_tag="IXT20_001798"
     CDS             12655..13143
                     /locus_tag="IXT20_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAQ4860237.1"
                     /translation="MFLKVGTGIAYIKVNRKKGINHMKKILIATHGHLASGFLSSIQL
                     LTGKVQEITVINAYVDECDFEQELNVFVNQVNEQDQIFVFTDLFGGSVNQKITKTFLE
                     KEIAVTILSGFNLPILLEIVLATKELTKEDIQELVNKCQQELKMTILDEVHVVNEEED
                     FF"
     gene            13155..13649
                     /locus_tag="IXT20_001799"
     CDS             13155..13649
                     /locus_tag="IXT20_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ4860238.1"
                     /translation="MIKAVRIDERLIHGQVAMTWTKSLNLTGLVVASDEAATNDIQKM
                     TLKMAAPSNVKVIIKTVDDAIHLLKDPRAKDMRLMVLVPNVKDAVKVAKAFPTEIELM
                     NVGNAGKMTPPSEDKKTLSKEVMLTTSEIQDLKELVELYPDTVFQPTPAMEKRSAISI
                     LSDF"
     gene            13670..14479
                     /locus_tag="IXT20_001800"
     CDS             13670..14479
                     /locus_tag="IXT20_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ4860239.1"
                     /translation="MYDAFVVALAVFIGVAGHEMFGMSMLSRPIVVAPLAGALMGDLQ
                     TGLIVGASLEAIFMGVVNIGISSTAEPSLAAGLATAFAIKMGGNVDAIIPIVFPLAVL
                     GLQLMNMIFSFVCGPMAAKFFKFAKSGDQKGIINLHFFMWFVHYALYALIPFFAVLFG
                     ADVVKSVLDNIPEVIMNGLTVAGNLLPAVGMAMLLRMLWSKNIAVWFFFGAVVMAYLE
                     LPLIAIAAVGTIIAIVIAQRDLQLKNISVHAVQSDAAVNATLDQEEEDFFQ"
     gene            14476..15297
                     /locus_tag="IXT20_001801"
     CDS             14476..15297
                     /locus_tag="IXT20_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAQ4860240.1"
                     /translation="MKLADGISKDEKKLVRKMFWRSMTMYASVNPVTMGGGGFAYSMM
                     PFINHFYKSEEDRAAALERHTQYFSTTIPCASFVMGIAASMEKENSEKAGFDVESINA
                     IKTSLMGPLAGIGDSLFWGVWRVVTAGLAINLANAGNILAPIIFLVMFNIPNYLMRYY
                     GGVLGYSLGSKYIEKLYANGLIEILTKAASILGLIMVGAMTISMVKFNTVLSFSMKGE
                     ELMNVQEILDSIFKGIVPLLVTLSCFKLLDKKVGVIKIIFGMVVLGILLSLLGIA"
     gene            15348..17447
                     /locus_tag="IXT20_001802"
     CDS             15348..17447
                     /locus_tag="IXT20_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase"
                     /protein_id="HAQ4860241.1"
                     /translation="MTINEVIYSPKHPFFNAELNDGNVTTSNEYSYYRNIPDVKGLTF
                     DVSNQKTLANLDVCGTIQTLTFYRENLLTEEKPGVWVNKQLSQTNSLSLKVEVNGIVY
                     HLSESDHRIEVDVIQDCLPRYIHHYSTFKVIVVSFAPILANKRLSMLVQQVYIVNETH
                     ESLQVSVKDVPSYQEKYSDQQNVLISQKGNKDTIAQQEVASFAIAFVDPNAFEEVMTF
                     QESDIEKWLKETLNYFAQLFGQLSMPNDRVVHLYRRALYQSFSSFGMNRQGQVVGSNW
                     GSYPATNRIWNKDMYYASLPFTMFEPELCQKTVLWFDQYGVKFPGSKFTGGINHSLSN
                     SLSSALLASLYFEHTGDTKFFERCPQVLLNASRIIEDILAQRRSGEPMLFPSVWLSDA
                     FALGKYHTGSNLCLWKACIGLAEIFEALEQLSLSKRYKNIACKLKESICKQMTIDGPF
                     GEQFLEGIGDEEKTTYSVKHYQKPILEQGLIFLSDVIVDGKINLLMHDGEESDTTLMP
                     FYQFLDNKDHLYQNTMAFSASSFNPTYSEEIKGITWGLESGATFPGFITVLMSELHNP
                     QAFVTRLEELICLADLDGSWWWWPYRLEAQQGEVVRDFGCGKCGWASGLFVSLMISQY
                     FGLKFKKGVLQIDPVDNLTFSWKNLKFGQAFISIECTQSELSISNLSEKPLKISDTKK
                     ILHVEKGKTIHIQRRKR"
     gene            17444..18562
                     /locus_tag="IXT20_001803"
     CDS             17444..18562
                     /locus_tag="IXT20_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2961 domain-containing protein"
                     /protein_id="HAQ4860242.1"
                     /translation="MSILKNITQFKKIQSRAITPENPTGEKGKAAMLPSELGPSRKGR
                     GAIPLTSGEETVIADITGTGIIRHMWMTIRDRTEFGSFVLRDLILRIYWDGSDSPAVE
                     SPLGDFFCNGFGERCEVNSLPIVVNPTGGFNSYFEMPFQTSAKITIENLHPKDMSSFF
                     YTINYALVDELEENSLYFHANWHRERITKLQEDYKLIDRLKGQGYYVGTYLALTCLER
                     YWWGEGEFKFYIDGDSEYPTVTSTGSEDYFGGAWAFHEYDEFGRPHAKNYSTAFLGYP
                     YQSNRDATRDRFETGKLNAVHAFGDDAMPRHGLYRWHLQDPICFTEDLSVTLQQIGND
                     DVRLFERQDDVASVAYWYSTEASGNTKRHFTDRERLPR"
     gene            18601..19479
                     /locus_tag="IXT20_001804"
     CDS             18601..19479
                     /locus_tag="IXT20_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAQ4860243.1"
                     /translation="MIYGAIEAGGTKFVCAVGTEELTILERVSFPTTTPVETMKQVID
                     FFKPFKEQLAGIGVGCFGPIDIQCKSTSFGHITSTPKLAWQNFNFVGTLKQYFNIPIE
                     WTTDVNAACYGEYVAGSGKGLAGVAYFTVGTGIGGGALFNGTFVEGFSHPEMGHMLIK
                     RHPKDNFSGNCPFHQDCLEGMAAGPAIEKRLGVKGQNLLADDSFWQIEAFYLAQCAYN
                     TTLMFSPDRIIFGGGVMKQEHMKKKVQDKFVELINGYVEIPPIDSYIITPELGDNAGI
                     IGGLALARKAVRNKQP"
     gene            19670..19822
                     /locus_tag="IXT20_001805"
     CDS             19670..19822
                     /locus_tag="IXT20_001805"
                     /inference="COORDINATES: protein motif:HMM:NF016526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4860244.1"
                     /translation="MRIFTGGVHLNSNILCYFMSVVIVIVAYQCMFIFRNVDFIILGL
                     MFEFFW"
BASE COUNT         6108 a         3482 c         4280 g         6139 t
ORIGIN      
        1 cggaaaaaga gcgtgggaca aaagtaaaga acacttttgt ctcacgctct aaacacgtat
       61 aaacggcgga agcagaagaa attccttcgg aaataagctg aaattcacaa aaatttgaaa
      121 agcaattttc gtgaatttct tcttatttct tggagttaaa cgcttctgtc ccagcctctt
      181 ttttgatcag catgatttta ttcagttatc ttcaaaatga tttttccgat gtttttgttt
      241 gcttccatat attcatgcgc ttgtttgact gcgctgaatt ggaatgtacg gtcaatgatt
      301 ggccgaatct tgccattttc tagaaaaggc ccagcttttt gcataaaagt ttgtgcgagc
      361 tctgctttat actgatcact tctaggtgtc aataatgtac caagcagttg gacacgtttc
      421 ccaatcaata caccaaggtc tatctcaggc acgatggtcc cacctagcat accaatcaag
      481 acccaacgtc catcaacggc tatagaagct aaattctttt cccagtaaga agcgccgatg
      541 aaatccaaaa taacatcgac tccccgacct tcagtgattt ttcgtacctc ttcttgaaag
      601 tcttgttttt tatagttgat caattcatct gcaccaagtt ctttacaaaa ggcgagtttt
      661 tcttctgatc ctgcagtaac gatgattttc gcgtgagaaa gctgtctagc taattggatc
      721 gctgctgttc ccacaccact tgctcctgca tggatcaaga cagtttcgcc ttcttgtaat
      781 tttccaagcc agtaaagtgt ttgataagca gtcaaaaaaa cttctgagat tccagcagca
      841 gaaacatagt ctagcgtatc agggagaagg attgcacgat cggcaggcat cacagcatat
      901 tcggcataac cacctagatt gactaatccg tatacttttg tcccaggagt aaaagtggag
      961 atatcacttt tattctcaac tacctctcca gctacttcga ctcccaaaat agggtaggga
     1021 ggtttcaaag agactgattc tcttttcata atatctgttc gattgacagc tgctgtatgg
     1081 actttgatca gtaattcgcc ttgttttggt ttaggaacag gcacttcaat ttgttctaat
     1141 acatctacag aaccaggatg cttgattgaa atggcacgca tggatctatt cctcctttgc
     1201 ccattcttgg aatgctgtat tcaactcttt gctaccttca gaaagtacta gagtcgtacg
     1261 agaaatatcc gtttcttcca ctagagcggc aagtacagca gcaactgtag agcgagtgat
     1321 cgtttttgat gccttctcat taggcttagt taattgaatg gaagtaactt cttcatcgtt
     1381 agtcaatgtg actggttgaa gaatgacaaa atctaagtta gttcgtttca gccattcatc
     1441 tgcataatgt ttagtaatat aataatctgt catggactca ggccattttg ctggttcatc
     1501 tgcataaaca gcactgacca tcagatagcg agagacatgc gcattttcag ctgcgatcac
     1561 tgttttgatt gcgccatcca agtctacttg cagaagattc tttccttgag agccggcagc
     1621 aaaaatcaat acatcgatgc ctttgaatgc ttcggtcatt ttttccacgg accaagttaa
     1681 atcaaaagag acatacgaaa cattctcttt ttttactaat gtttgggaag caacatctct
     1741 cacccctgca aaaatttcat gggaacttgg agctaaatct tttatcaggt gtcggccaat
     1801 ttgtccgttt gcacctacaa caaaaatttt catttggatc atcctttcga cgtcaaatct
     1861 ttccattcaa tagtaacacc aaaagagaaa aaaatcagag atcggctacg atgactatca
     1921 gttagtaaga gaagggtgct tgaagtatct taacaagaca ttgcatactg ctgcagtcgc
     1981 aagacctgcg acgattcctt caggtatacc acttgtaaaa ataaccgcta gaatagctga
     2041 ataaacagca tttaaatttg ttccgatgac tttggcataa tcttgctgga ataaaaagta
     2101 gatcatattc atcactaaaa tcgtattggt caatgaaccg gaaagtccag ctaagaataa
     2161 agaaacggaa tctgcttttt gtttcattag tttgcgcaga cctttataaa cgaaataagg
     2221 aaccacccca atcaagatgc gaggaataaa cgcgatcagc aaggctttcc agcttccatg
     2281 ttctgttccg atgactggga taaacggaga aaagacaaaa gacaaaggtg tttggataac
     2341 tgtgctacgg atcatgctga tcagtccaaa tgtaccgcca agaaaagcac ctagtcttgg
     2401 tcctaaaacg atcgaagcaa tgattacagg aatatgcatg gttgttgcat tgattggacc
     2461 gatagggata aaacctaaag gcgtaacagc taataaaatc aaaatagcta aaaacatcgc
     2521 agttaatgta aagttttttg tatttttcat aagtactcct tttttctaag catttctttc
     2581 attaagagtc agcatgacct gttcgatgat cgtctgaata tcagctaagg caccttttcc
     2641 aaaatctcca caagctaaaa gagattctct tggttcaatt tcctgatagc cgacagatct
     2701 caatgtttcc agattacgct gagtgatcgg gttttgatac atattcgtgt tcatggcagg
     2761 ggcaatcaat tttggtgttc ccactggaag cgccattgcg actgttgaaa gaagatcgtc
     2821 tgcaatgccg tttgctagct tgccgataat gtttgctgta gcaggcgcga tgagaaacaa
     2881 gtcagcttgt tttgctagtt cgatatgatt gattacctgc ggatttgatt cttgcatgac
     2941 gtctgtatgg actgcgcgct tcgacagaga ttgcaaggtt aatggcgtga taaaatgtgt
     3001 gctgctttta gacataatca catctacctg ggcacctaat ttgacgaatt gacttgtaag
     3061 atcagcgctt ttgtaagcag aaatacttcc agtaacgcct agtaagatct ttttcatttt
     3121 gattcctcca gtctaatttt ctcagtaatc aatgcagcaa tctcagattt actttgagct
     3181 tcttccactg ttccatcttt agataagaga tagccgtggt gtgtctcgcc atggatttcc
     3241 gtcaaatcat ttgcgaaaat aaaatcagtt ttgttattta tcagactatt ttttgcgact
     3301 gccagaagag attcctttgg tacatctact aatagtttga aaccaatgat ttttgctttt
     3361 ggctgccatt cacgtagata agaaatgatt tttggtgttt tcttcaaaac gagcacaaga
     3421 tgctcggtgt tagaagagat ctttgaaaca gtggtatctg tctgttcatt ttccgcaaac
     3481 caattgtcca gttcttcttg cgttgaagaa gtgggtaggt tttttgccag ctgttcttct
     3541 gaaaaactaa aagcaggagt aaaatcactg actgccatac tgtggataat cgcatcgtag
     3601 tgttcttttt tcgttagttc ttctaattgt aaaaataagt ccagtgcaga ttcgatcgta
     3661 taaatcgtta tattggagtg tcttttcggt ttcagtgctt gtttcgtagt cacgtaatcg
     3721 attgtggtat ccggattggc agctaaataa tcagctaatg cttgccctaa tcgtcctgtc
     3781 gaatgatttg taatagcacg tacttgatcg attttttcag tcgttcctcc agctgtgatt
     3841 agaatcttca tttatggtca cctacattta ctagtgttct tccttggtgt gttccagcaa
     3901 gtagtttaga tgctgtttca ggcaattgat ccagagtaat ctcttggacg accagttcat
     3961 ctgcaatctg caaatccgcc atctgttgcc ataaagaaag acgttgatct attggtacat
     4021 tgacagaatc aattccaatc aactggatgt ttcgcagaat aaaaggcaag acagtcgcgt
     4081 ttagtttgat tcccccagca ttgccacata aagctactgc accgttataa gagatcaatg
     4141 gaagaagact gcttagctgc tcgccaccta ctgtgtcgat gacatagtcg atttgctgtt
     4201 ttcctaaagc ttttgttgtc tctggcagaa attcatcagg tcgtacgact tgcaaagcac
     4261 ctagactttt tagccatgtg acagactctt cttttctgga aaaggcaatg attgactgga
     4321 atcctagttt atgtaacagg gctatcgctg tactgcctac accgccagaa gcaccagtaa
     4381 cgacaacttt tttatcggag gtcatacctt gacggattaa agcgttcaca cacaacatag
     4441 cagtaaaccc tgctgtacca aggatcatcg cttgacgcag cgtcatattt tttggtagag
     4501 gaactaacca gtctcctggt actttttgat attggctata accgcctgga tgggtgacgc
     4561 caagcccaaa accagtagca agtacaagat cttcttttga aaaacgtgga ttgttacttt
     4621 ggacgacagt tccggataaa tcaatccctg gagtcatcgg atatgttcgt atcacctgac
     4681 cagactcgct cattgccaat gcatctttat aattgacatc agaataggca atttttacca
     4741 agacttcatt ttcagccaac tcagtaaatg gcagatcact gattgctgta tggaaaacag
     4801 gttcctttat tacctgaaaa gatcgaaaag cgttcattcg tgagacactc cttttaaaat
     4861 tttcttttat cataccataa agcctactct tttattcttg ttcttccgta aatttagaca
     4921 aaaattaggt gaaaaataaa gaatcaaaca actagaattt attccaaaag ataaagaaat
     4981 attcagcaaa aaaaatttta caaagggttg cctttttcaa taaatttcgt atactaatac
     5041 gagtggtagt aaacaggaag tttactatta gaaaacttcg attcggagga aattatggaa
     5101 attggtgaga agttaagaaa tttgcgcatc caaaagaatt tgacacaaga agaactaggc
     5161 gaacgcacag atttgtcaaa agggtatatt tcccagttgg aacgagattt aagttcacca
     5221 tcaatggaaa cattcttttc tattcttgaa gtcttaggtg tgactccaga agaatttttt
     5281 catcaagaag cagcgaatct gcaagtggtt tattcaaaag aagatcatac ggtttatttc
     5341 gatgaagaaa acgggtatga attggaatgg ctcgtggctg attcgaatga aaaagaaatg
     5401 gagcctgtct tgctgacttt tgaaggtgca ggagaatata agacgtttga accttcatta
     5461 tctgaaacgt ttatttttgt attagaaggg gaattagagt taagcttagg agagacggtc
     5521 tatgtggcaa aagcaggaca gtcgatctat tatcaagcga caaaacaaca tcaattaagg
     5581 aatcatctca aaaaacgaac aaaagtattg attgttgcaa cgcaatcata tttgtaatat
     5641 agggggtaaa aacgtgggga aaccaatcat ttcatttgat caagtagtga aacgctatga
     5701 tgatgaaaca gtattaaaaa aagtaagttt tgagatcgaa caaggaaaat tttatacact
     5761 gcttggtcct tccggctgtg gtaaaacaac gatcctacgt attatagctg gattcaccga
     5821 agctacagaa ggggacatct attttgaagg gaaacggttg aatgatttgc cagctaataa
     5881 gcgacaagta aatacagtct tccaagatta tgcacttttt cctcatatga atgtttttga
     5941 taacgttgct tttggattga agatcaagaa aatgtccaaa tcagatatcg aaaagaaggt
     6001 aaaagatgct ttgcgtatgg tccagcttcc tggatacgaa aaacgggaga ttagtgaaat
     6061 gtctggtggt caaagacagc gtgtagcgat tgcacgagca attgtaaatg aaccgaaagt
     6121 attgctatta gatgaaccgt tatcggcgct tgatttgaaa ctacgtacag atatgcagta
     6181 tgaactgcgg gatctgcaac aacgattagg aatcacgttt atttttgtta ctcatgacca
     6241 agaagaagcg ttggcaatga gtgatgagat ttttgtcatg aataaaggga aaatcgtcca
     6301 aagtggtaca cccgtagata tttatgatga gccaatcaat catttcgttg ctgattttgt
     6361 tggggagagc aatatcgtgg acggtgtaat gatcgaagat aatctagttg agtttgttgg
     6421 taagcaattt gaatgtgtcg atggcgggat gcgtctaaat gaaccagttg aagttgtttt
     6481 acgcccagag gatttgacga tcacgacacc agataaagga aaactagtcg taacggtcga
     6541 tactcagctg tttcgtggtg tccattatga aatcatctgt tatgatgaac agcaaaatga
     6601 atggatggtc cattcaacga aaaaagctaa agaaggaagc aaagtaggat tggcctttga
     6661 gccagaagat atccatgtca tgcgtttcaa cgagtctgaa gaagaattcg atgcacgtct
     6721 tgacagctac gaagaatagg ggggacaaga atgaaaacaa tgcgcaggat ctattccgtc
     6781 ccttatattt tgtggctcgg tttattcgtg atcgcgcctg tattaatgat catttatcaa
     6841 tctttttttg atatgaatgg gcaattcacg ctgaataatt atctatctta ttttacgtcg
     6901 gggacatcgc tttctatgac gatcaattct gtatggtatg catttttgat cacgtttttc
     6961 acgctgatca tcagttaccc gactgcttat tttttaacaa aactgaagca caagcaatta
     7021 tggctgatgc tgattatttt gcctacatgg gtgaatctgc tgcttaaagc ttacgcattt
     7081 attgggattt tcagtgtgca cggcagcgtg aatcatttct ttgattttat cgggatcggg
     7141 ccgaaacaga tattattcac agacttcagt tttctgttcg tggcaacgta tatcgagatt
     7201 ccatttatga ttatgccaat tttcaatgca ttggaagaga tgaatccttc tttgatcagt
     7261 gctagtagag atttaggcgc gaatagcttt gagaccttcc gacgagtgat ttttccgtta
     7321 tcattaaatg gtgtgaaaag tggtgtccaa gcagtattta ttccttctct ttctttattc
     7381 atgctgactc gtctgattgg cggaaatcgg gtgatcactt taggaacggc tatcgaacag
     7441 catttcttgg ttacacaaaa ctgggggatg ggttctacaa tcggtgtgat tttaatcatt
     7501 gctatgttca tcgtgatgct cctgacaggc gagaagaaaa aaggaggtcg ctcataaatg
     7561 aaaaaattta aatggtcgta tctttattta gggatcgtct ttttattctt atatgcaccg
     7621 atcttctatc tgatttttta ttccttcaat gaaggaggaa cgatgaacag ctttacagga
     7681 tttacttggg aaaattatga agctgttttt gaagatacac gtttgatcat tatcgtattg
     7741 aatacgttca tgctggcttt cttatctgca ttgatcgcca cgatcatcgg gacatttggg
     7801 gctatgggaa tctattacac gaaaaaacga cgtgcacgga cagcgttgtt gagcatgaat
     7861 aacatcttat tagtctctcc tgatgttatt atcggtgcta gtttcttgat tttctttaca
     7921 ctactaggat tcggattagg ttttcagagt gtattgcttt cccatattgc tttcagcatt
     7981 ccaatcgtcg tcttgatggt cttgccaaaa ttgcaggaaa tgaacgattc aatggtcgat
     8041 gcggctagag accttggtgc taataatttc caagtagtca aaaatatcat tttgccttat
     8101 ctttctccag gaatcatagc tggatatttt atggcattta cctattctct tgatgatttt
     8161 gcggttacat ttttcgtcac aggaaatggc tttacgacgc tctcagtaga gatctattct
     8221 cgtgcaagac aaggaatcag tttagaaatc aatgcgctaa gtgcgttagt tttcttgttc
     8281 tccatgatat tggtcattgg atactacttt attagtaaag aaaaccgtcc aaaacggctg
     8341 aagaaaaaca gaagagtacg cgagggggtg gcacggctgc gatgaaaaaa ttacaatctc
     8401 tgtttttagg tattctcatc attattgcgg tgctgctgct tggtgttcgt caacttgaaa
     8461 aaacaagtgg catgagtgga gcaaaggtac ttaccatcta taactgggga gattatatcg
     8521 atcctagttt gatcacgaaa tttgaaaaag aatatggata caaagtgaat tatgaaacat
     8581 ttgattctaa tgaagcgatg ttcacaaaaa tccagcaagg tgggacaaac tacgacatct
     8641 ctataccaag tgaatatatg atccaaaaaa tgatcaaaga aaaactagta cttccattag
     8701 atcattcgaa gatcaaagga ttaaataata ttgatccacg atttttagac ttggattttg
     8761 atccagataa tcagtattcg attccatatt tttggggaac tttagggatc gtatataacg
     8821 ataaattctt tgatgcaaat caaattaagc attgggatga tttgtggaag ccggaattaa
     8881 aagacagttt gatgctgatt gacggtgcac gagaagttat ggggctttct ttgaatagtt
     8941 taggctattc tttgaatagt aagaatatgg cacagctgac agaagcagca aataaattga
     9001 atcatttaac accaaatgtc aaagcaatcg tggcagatga gatcaaaatg tatatgatca
     9061 atgaagaagc ctccgtggca gtgacatttt ctggcgaagc tgccgatatg atgtgggaaa
     9121 atgaacattt acattatgtg atcccaccag aaggatccaa tctttggttt gataatatcg
     9181 ttatgccaaa aaccgtgaaa aataaagaag gtgcctatga tttcatcaac tttatgctag
     9241 aaccggaaaa tgcggcacaa aatgcagagt atattggtta ttctacacca aatgaagaag
     9301 caaaagcgtt attgccaaaa gacatttcgt cggacgaaca attctatcca tctgatgaca
     9361 cgatctccca tcttgaagtc tacaaagatc taggatcgaa atacctaggc atctataatg
     9421 acctattctt agaattcaag atgtatagga gataatggca aaactcagta agaatgggga
     9481 cttatcaacg ttctaaagag ctaaaaatcg tcctttttct atctgagttt tccaatcttc
     9541 taccaaaagc ggatgaaacg ccgctttttt tgtgttattt tattttttct accacgtttt
     9601 tctaccagag aaaagtggac aaaaccattg taaaataggg cccgagacgg gctagtaaat
     9661 ctaagattga aaaagttaca gaaggataaa gtaattggaa actatgcaaa tatacttaac
     9721 ccttctgtaa tctattaata aaactaatat atgaatccag ttcaatattt gtagctatga
     9781 aaaaacataa aaatcatatt agataaaaac taaattggta gataaattat tttgtcagat
     9841 atctgaggca aatattcgac tatttgtgtt tcaaagtaaa aattggaata caaatataga
     9901 atagacatat tgcagccgat agtttattat gcttttagac acattaaact ggaggaaaaa
     9961 atgaaaaaga gcgctaaaga tcgagttttt gatgagctga tacgactaac agggaatatg
    10021 tcttctgtca ctgccgagaa aatttcgaag aatctaggta tctctcgtca gaatgccagc
    10081 cattatttga cgagattagt ggaagataaa aaggtcgaga aattaccggg aaagcctgtt
    10141 ttgtggaagc ctttagatga atatgcggtt gtagataata cggagcaaat aaacgaagcc
    10201 tttcattctg tagttggtca tgacggaagt ctgagagagg tcattcaaaa atgtatagcc
    10261 gctgtcaaat atccgcctaa cggactcagt gtattaataa atggagccac gggtgttgga
    10321 aaaagttttt tagccacaaa aatttttgaa tacgctatcc atgagcaaat tattgaaaaa
    10381 gatgcaccat ttgctatctt aaactgtgcc gattatgcgg acaatcccga actgctttcg
    10441 gcaacactgt ttggatacaa gaagggggcc tttacggggg ctgaaaagga taccgatggt
    10501 ttattggcaa cggccaacaa cggctatctt tttctcgatg aggtccatcg cctatcgaaa
    10561 gaaaatcaag aaaagctctt tctatttatt gatactggaa actatcgtcc agtcggtgaa
    10621 aatgtaaatt ggcattctgc taaggttcgt tttattttcg ccacgacgga aaaaggtgaa
    10681 aattatttat tagacacctt tgatcggcgt attcaaatta gtgttatgtt gccaactttt
    10741 gaagatcgcc ccattcgaga acgattagag ctgattcaat tattttttca aaatgaagcg
    10801 gatgttttac aaaaagatat tattgtttca agagaagcct tgtctttaat gttatctcat
    10861 ccctttagtg ggaatattgg taagctgaag aatatcatta aaatcagttg tgcggatgtg
    10921 tatagtcgaa cacaagaaga accattaacg attgggaaaa atgagatctt gattcagtta
    10981 aatatgctgg aatccgaagt agaaagcttg ccgattgcta gtttattgat aaagaaaaat
    11041 caaaagataa aaacggatcg aatcaaaccg tttgattatg attcgatttt ggaaaaaatt
    11101 actcagctga taaatgaagg taatttcaat gaaaattttt cttcgataaa gaatgaagta
    11161 cagcatttgg tgcatcacat tgataaggat tcttttttat caatgagtag aaaattgatg
    11221 attcagatgt ttagaaaggt ctgggtatcc gttttaggaa aaacgtacgg attgacgacg
    11281 agtggacctg ttgcagaaat tattagcaga tcctatgtca tttccccaag ctataagaca
    11341 gacttaaccg ggataaatcg tacattagct gcaaatctac ctcggacgac ttacttatgt
    11401 aatcagttct tggagcattt accaccgctc tcagaaaagg acgagaactt tctaagactg
    11461 cttattgtcg tgactttatc tgattatgta gatgaatcca tcgagctgaa gggcctgttg
    11521 cttgctcacg gagatagtac ggctagcagc attcaagcag tagtgaatca attatgcggc
    11581 aattatgttt ttgaagcgtt ggatatgcca gtagagagta atgtgtcaga gattgtcgaa
    11641 tcgacaagga atttcgtgaa acggcaagtg aatacagata gtctagtgct gatcgtagat
    11701 atgggctctt taaatcaaat ttacacacag atcaagaacg atttaaaagg ggagttactt
    11761 cttctgaata atttgacgac ttccattgca cttgatattg gcttgaagat gacgatgaat
    11821 gcgccattta aacagattgc ggaggatgca gcagacaagt attcaatcaa tgttcaatac
    11881 tttgaaggct tttctcaaag tagcaacatt atcatttcct gtatgtcggg tttggggatt
    11941 tcggacaaat tgaaagatgt ttttcgtcat catttatcgg atgaatccat cgaagttttc
    12001 acgaaggact acagagaact acgtgaatta atcgaccaaa atgatgaaac ttattttgac
    12061 aagacaaaat tggtcatcac gacttcggat cttccacgtt ccttttcaat tcctaatatt
    12121 aatgtttatg acatcttaga cggtgatgga gcagccaatt tggagcatat tttgagtcct
    12181 gaaatgaagc cggcagaatt taaccgcttg attcaggagc tagtaaaatt cttttcgttg
    12241 gaaggaattg cggaccgttt gaactttctg aacccaacga tcgtgatgag tgaagtggaa
    12301 acagtcattt ctaaatatga aaattactat catttcactt taaatggccg tgtaaaacta
    12361 aatctctata tgcatacggc attgatgatg gaacgtttat tcttggctcg gacagaaaaa
    12421 tctgaagaac cgaattatga tttgaaacca gatgaagatg aattttatta tgtttctaga
    12481 agtatttttc aatcaatgga gatgaaatat aattttaaag tcaatgctta tgagctttcg
    12541 ctgctctatg aattattagg accgtatatt caaaaataaa gtgtctaaaa aagtgtctaa
    12601 tctgtttttt taggttagac acttttttta atgtgaatta acgtgattaa accaatgttt
    12661 ttaaaagttg gcacaggaat tgcttatata aaagtgaaca gaaagaaagg aattaaccat
    12721 atgaagaaaa ttctaatagc gactcacggt cacttagctt caggctttct tagcagtatt
    12781 caattactga cggggaaggt tcaagagatt acggtgatta atgcttatgt ggatgagtgt
    12841 gattttgaac aggagttgaa tgtatttgta aaccaggtga acgagcagga tcagattttt
    12901 gtttttaccg atttatttgg cggaagtgtc aatcagaaga ttaccaaaac ctttttggag
    12961 aaagagattg ctgtaactat tttgtctggc tttaatctgc caattctgct cgaaatagta
    13021 ttggcaacca aggaattaac aaaggaggat attcaggaac tggtaaataa atgccaacag
    13081 gaattaaaga tgaccattct tgatgaggta catgtagtaa atgaagagga agattttttt
    13141 taggaggaag aaaaatgatt aaagcagtgc gaattgatga acgattaatt catggacaag
    13201 tagcgatgac ttggacaaag agtctaaatt taaccgggtt agtagtagca agcgatgagg
    13261 cggctaccaa cgatattcaa aagatgacat tgaagatggc tgcaccatcg aatgtcaaag
    13321 tcattattaa aacagttgat gacgcgattc atttgttaaa agatccgcgg gcgaaggaca
    13381 tgcgcttgat ggttttagtc cctaatgtca aagatgcggt aaaagttgcg aaagctttcc
    13441 caacggaaat cgaattaatg aacgtgggga atgctgggaa aatgacaccg ccatcggaag
    13501 ataagaaaac cttatcaaag gaagtgatgt taacgacgtc agaaattcaa gacttaaaag
    13561 aattagttga actttatcca gataccgttt ttcagcctac accggcgatg gaaaaacgtt
    13621 cagctatctc tattttgagt gatttttaga aaaaggaagg agaaaaatta tgtatgatgc
    13681 atttgtagtc gctttggccg tttttattgg agttgctgga catgaaatgt tcgggatgtc
    13741 catgctgagt cgtccgattg ttgttgctcc gttagcagga gcgttgatgg gggatttgca
    13801 gacggggttg attgtcgggg cgtcactaga agccatcttt atgggtgtcg tcaatatcgg
    13861 aatctctagt acggcggagc cttctttggc tgctgggctg gctacagcgt tcgcaatcaa
    13921 aatgggagga aacgtggatg cgattattcc cattgtcttt ccactagcag tattaggatt
    13981 acagttaatg aatatgattt tttcttttgt atgtggaccg atggctgcta aatttttcaa
    14041 atttgcaaaa tctggtgatc aaaaaggaat catcaatctg cacttcttta tgtggtttgt
    14101 ccactatgcg ctttatgctc ttattccatt ctttgccgtt ttatttggag cagatgttgt
    14161 caaaagcgtc ttggataata ttccagaggt catcatgaac ggattaacag tcgctggaaa
    14221 tctgctgccg gcagttggga tggcgatgct gttacggatg ctttggagta aaaatattgc
    14281 tgtgtggttt ttctttgggg ccgttgttat ggcgtatctc gagttaccat tgattgcaat
    14341 tgccgcagtt ggaacgatta ttgcaattgt tatagcccaa agagatctac aactaaaaaa
    14401 tatatctgtt catgcagttc aaagcgatgc tgctgtaaat gcaacgcttg accaagaaga
    14461 ggaggatttc tttcaatgaa attagctgat ggaatttcta aagatgagaa aaaattggtt
    14521 cggaagatgt tttggcgctc gatgactatg tatgcttcgg tgaatccggt gacgatgggc
    14581 ggcggtggtt tcgcctattc tatgatgccc ttcattaatc atttctacaa aagtgaagaa
    14641 gatcgagctg cggcgctgga acgtcataca cagtattttt cgacgaccat tccttgcgcg
    14701 tcctttgtca tggggattgc ggcatcgatg gagaaggaaa attctgaaaa agctggtttt
    14761 gacgtggaaa gtatcaacgc gatcaaaacg agtttgatgg gaccattggc tggaattggc
    14821 gattcattgt tctggggagt ttggcgtgtc gttaccgcgg gactagcgat caacttagcc
    14881 aatgccggaa atattttggc accaattatc tttttagtta tgttcaatat acctaattac
    14941 ttgatgcgat actatggcgg cgttttaggc tattcattag gctctaaata tattgaaaag
    15001 ctgtatgcga acgggttaat tgaaattctg acaaaagctg ctagtatctt gggtctgatc
    15061 atggtaggtg caatgacgat cagtatggtc aaattcaata ccgttttgag cttttcaatg
    15121 aagggcgaag aactaatgaa tgtccaagaa atcctcgata gtatttttaa aggaattgta
    15181 ccgttattgg taacattgag ctgtttcaaa ttactagata aaaaagtcgg cgtcatcaaa
    15241 attatttttg gaatggtcgt cttgggaatt ttattgtcat tacttggaat cgcatagtag
    15301 gaagctgcag ctactaggct gcagtttttt tagcaaggag ggaaaagatg actattaatg
    15361 aagtaattta ttcacccaaa cacccatttt ttaatgctga gttaaacgac ggaaatgtga
    15421 ctacttcaaa cgaatacagc tactatcgga atattcctga tgtaaaggga ttgaccttcg
    15481 atgtaagtaa tcaaaagaca ttagcaaacc ttgatgtttg cgggacgatt caaacgttga
    15541 ccttttatcg agaaaattta ctaactgagg aaaaaccagg tgtttgggtc aataaacagc
    15601 tctctcaaac caattcgtta tccttaaagg ttgaagtaaa cgggattgtc taccatttgt
    15661 cagaatcgga tcatcgtatt gaagtcgatg tgattcaaga ctgtttgcct agatatattc
    15721 atcattattc gacgtttaag gtcattgtag tatcctttgc tcccatactg gctaataagc
    15781 gtttaagtat gcttgttcaa caggtttaca ttgtgaatga aacacacgaa agccttcaag
    15841 tttcagtgaa agatgtgcct tcgtatcaag agaagtattc ggatcagcaa aatgtgttga
    15901 tttctcaaaa aggaaataag gacacgatcg cacaacagga agtggcaagc ttcgccattg
    15961 cttttgtgga cccaaatgct tttgaagaag taatgacttt tcaggaatct gatatcgaaa
    16021 aatggctgaa ggaaacattg aactattttg ctcagctatt tgggcagctg tcaatgccaa
    16081 acgatagagt tgtccatctc tatcgtcgtg cactttatca aagcttttct agtttcggta
    16141 tgaatcgaca gggacaggtc gtcggcagca attggggcag ctatcctgct actaatcgaa
    16201 tttggaataa ggatatgtat tacgcatcat tgccattcac catgtttgaa ccggagttat
    16261 gtcaaaaaac ggttctatgg tttgatcaat atggcgttaa gtttcctggt tcaaaattta
    16321 caggaggtat caatcattct ctgagtaatt cgttatccag tgcgttactg gctagtcttt
    16381 attttgaaca tactggggat acaaaattct ttgagcggtg tccgcaggtt ctactgaatg
    16441 cttcaagaat tattgaggac atattagctc aacgtcggtc aggggaacca atgctttttc
    16501 catctgtttg gttatcagac gcctttgcac ttggaaagta tcataccggc agcaatcttt
    16561 gtctttggaa ggcctgtatt ggacttgcag aaatttttga agccctagaa caactgagtc
    16621 ttagcaagcg atacaaaaac attgcctgta aactaaagga atcaatttgt aaacaaatga
    16681 ctatcgatgg tccttttggt gagcaatttt tggagggaat cggtgatgag gagaaaacaa
    16741 cctattcagt gaagcactat caaaaaccga ttcttgagca agggttaatc tttttaagtg
    16801 atgtgattgt ggatggaaaa attaatttat tgatgcatga tggagaagaa tcagacacta
    16861 cgttgatgcc tttttatcaa tttttagata ataaagatca tttatatcag aacaccatgg
    16921 ccttttctgc tagttctttc aatccaacct atagcgagga aatcaaaggc atcacttggg
    16981 gattagaatc cggcgccact tttcctggct ttatcactgt tttaatgagc gagctgcaca
    17041 accctcaagc tttcgtaacc cgattagaag aactaatttg tttggcagat ttagacggtt
    17101 cgtggtggtg gtggccgtat cgcttagagg ctcaacaagg agaagtcgtg cgagactttg
    17161 gctgcggcaa atgcggctgg gcatcgggtc tttttgtcag cctaatgatc agtcaatatt
    17221 tcggattgaa attcaaaaaa ggagtattgc aaattgatcc tgttgataat ttaacgtttt
    17281 cttggaagaa tttgaagttc ggtcaagcct tcatttcgat tgaatgtacg caatccgagt
    17341 tgagtatttc aaatttgtca gaaaagccat tgaaaataag tgatacgaaa aaaattcttc
    17401 acgtagaaaa gggaaaaacg attcacattc aaaggagaaa aagatgagta tactaaaaaa
    17461 tattacccaa tttaaaaaaa tccagagtcg agcaatcact cccgaaaatc caacaggcga
    17521 gaaaggtaaa gctgcgatgt tgccaagcga gctaggacca agccgcaaag gtcgtggcgc
    17581 aattccgctg acttctggtg aagaaactgt gattgcggat attactggaa cgggaataat
    17641 tcgtcatatg tggatgacga ttcgtgatcg aacagaattt ggcagttttg ttttacgtga
    17701 tttaatctta agaatttatt gggatgggtc ggatagccct gctgttgaaa gtcctttagg
    17761 ggatttcttt tgtaatgggt ttggagagcg ttgcgaggtg aactcgttgc cgatagtggt
    17821 taatcctaca gggggcttta attcctattt cgaaatgcct ttccaaacat ccgctaaaat
    17881 tacgatcgag aatttacatc cgaaggatat gtcctcattt ttttatacga taaattacgc
    17941 tttggtggac gaattggaag aaaatagcct atattttcac gcaaattggc atcgggaacg
    18001 cattaccaaa ttacaggagg attataagtt aattgatcga ttaaagggtc aaggctatta
    18061 cgtgggtact taccttgcgc ttacctgcct agaacgttat tggtggggcg aaggcgagtt
    18121 taaattctac attgatgggg atagtgagta tccgaccgta acctcaacag gttcagaaga
    18181 ttattttggc ggggcgtggg cctttcatga gtacgatgag ttcggacgtc cacatgcgaa
    18241 aaactattca acagcctttt tagggtatcc ttaccaatca aatagagatg ccacaagaga
    18301 tcgttttgaa acgggaaaat tgaatgccgt tcatgccttt ggtgacgacg caatgccacg
    18361 acatggtctt tatcgttggc atcttcaaga ccccatctgt tttaccgaag atttatcggt
    18421 cactttacaa caaatcggca acgatgatgt tcgcttattt gagcggcaag acgatgttgc
    18481 tagtgtcgct tactggtatt caacagaagc ttcaggaaat actaaacgtc atttcactga
    18541 tcgagaacgc ttgccgcgtt aatgaagtgt ttttcacata ttcgaaggga ggaacctatg
    18601 atgatatatg gggcaattga agctggaggg actaaatttg tctgtgcagt cggtactgag
    18661 gaattaacga ttttggaaag agtcagtttt cccacaacaa ctccagttga aacaatgaag
    18721 caggtcattg atttttttaa accttttaag gaacaattgg caggcatcgg ggtcggctgc
    18781 tttggtccaa ttgatattca atgcaaatca acgagtttcg gtcatatcac atcaacacca
    18841 aaacttgctt ggcaaaattt caattttgta ggaacgctaa agcaatattt caatattccg
    18901 attgaatgga caacagatgt caatgcggct tgctatggtg aatatgttgc tggttctggc
    18961 aaagggttag caggtgttgc atacttcact gtgggaacag gtatcggagg tggtgccttg
    19021 ttcaatggga ctttcgtgga agggttcagt catccggaaa tggggcatat gttgatcaag
    19081 cgacatccaa aggacaattt ttcaggaaac tgtcccttcc accaagattg tttagagggg
    19141 atggcagcag gaccagcgat tgaaaaacgg ttgggtgtaa aaggccagaa tttattggct
    19201 gacgattcgt tttggcagat tgaagccttt taccttgccc aatgtgcata caatacaaca
    19261 ctgatgtttt cgccagatag aattattttt ggcggtggtg taatgaaaca agaacacatg
    19321 aagaaaaaag ttcaggataa atttgttgag cttataaatg gatatgttga aataccacca
    19381 attgatagct acattattac gcccgaatta ggtgataatg ccggaataat cggcggatta
    19441 gcattggcca gaaaagcggt aagaaataag caaccataat taatgaatgt ttcattgata
    19501 ctcatgtcaa aagtatttaa ccgagtaatc aaatgatcaa atgaagttgg ataaagcgaa
    19561 aatgaattca aataaaaagg ctctaagtca aatcgtgttg ttttcattaa atcactgaaa
    19621 ggaaatgctt ctgcgtttcc ttttttcttt ttgttgtgcg ctgtatacct tgaggatatt
    19681 cactggtggc gtacacttaa atagtaatat attgtgttac tttatgtctg tggtaatagt
    19741 tatagttgct taccaatgta tgttcatttt tcgaaatgtt gacttcataa tccttggctt
    19801 aatgtttgaa tttttttggt gatagatttt ttcagccgta taaaataaat cgtaattcta
    19861 ttatagagag ttgtgattat aaaaagatag caagaaaaaa aaagaaagtt tctatacatt
    19921 ttaaatattt tattcctatt tctattgcta aaaaagtaga aattctatat ttacctttaa
    19981 ctctcgcttc tcttgcaaat gtagttagc
//
LOCUS       DACGLE010000046        20008 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0534
            SAMEA3304449-rid9560453.denovo.050, whole genome shotgun sequence.
ACCESSION   DACGLE010000046 DACGLE010000000
VERSION     DACGLE010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304449
            Sequence Read Archive: ERR985064
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20008)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20008)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702225.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702225.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 20:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,045
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 27 of 67
            Pseudo Genes (incomplete)         :: 25 of 67
            Pseudo Genes (internal stop)      :: 19 of 67
            Pseudo Genes (multiple problems)  :: 4 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20008
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304449-rid9560453.denovo.050"
                     /strain="VREN0534"
                     /db_xref="taxon:1352"
     gene            1..369
                     /locus_tag="IXT20_001806"
                     /pseudo
     CDS             1..369
                     /locus_tag="IXT20_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318369.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
     gene            complement(495..1574)
                     /locus_tag="IXT20_001807"
     CDS             complement(495..1574)
                     /locus_tag="IXT20_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAQ4860245.1"
                     /translation="MNNVFIFCYLEVVDLTVYQRFLANEKIRRLTVLLFVVFVLFLVR
                     SMITTILLTFIFTYLMIHLVQIIQRFVRIPTGVLAIMIYTLIVYLIYLAFTKYIPMLI
                     HQTFDMIQSVINFYEHQPKDADPVLQYIHTYIERNDFFEQLQNGASIALGYLQDIGKM
                     AVAFAMSFILSFFFMIEKKKTIVFSRLFLKSEFSWFFQDIYYFADKFVNTFGLVLEAQ
                     FVVALLNTLLTTIALAVFGFHQLLSLAIMIFILSLIPVAGVIISCIPLSFIAYSQGGI
                     RDVVYILITILIVHLIESYVLNPKLMSSKTELPIFYTFVVLLVSERFFGVWGLIVGIP
                     IFTFFLDVLKVKKIPNHLPKETDIP"
     gene            complement(1677..2117)
                     /locus_tag="IXT20_001808"
     CDS             complement(1677..2117)
                     /locus_tag="IXT20_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAQ4860246.1"
                     /translation="MEDLYLVNQVCFPLYAASKEIIRHYTPLLKPLNLTYTQYLVMLV
                     LWEKKKTSVKELGNDLCLDSGTLTPLLKKLAEKNYINRQRETGDERIVNVTLTAQGAA
                     LQKQAEGIPQKIKSYLPLEQEELDTLQHLTRKMLDNFHQDQKKG"
     gene            complement(2174..2584)
                     /locus_tag="IXT20_001809"
     CDS             complement(2174..2584)
                     /locus_tag="IXT20_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HAQ4860247.1"
                     /translation="MKKMYSTKMINTGGRSGEVHNPDRTFELNIAAPGKRVENATNPE
                     QLFAAGFSACFNSALELIKQQKNISGASTVSAQVSLYSESETSFVLGVEIEGSIEGLS
                     LEETQELLEAAHKVCPYSKATAGNIEVTLKAVSE"
     gene            complement(2764..3198)
                     /locus_tag="IXT20_001810"
     CDS             complement(2764..3198)
                     /locus_tag="IXT20_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HAQ4860248.1"
                     /translation="MHKGTEIDFVLNDSLKALELYQTIFDEIQLIEATDYPRGNNEVI
                     FTLYGTRFHMLDENTSLGLTAPTAEHPNTIWFNIIVSDIEDTHNKALRNGCVEIQEMT
                     EMMDFGVTNSMFRDPFGYLWMLHQVHREVIFEERKQLFEKYM"
     gene            complement(3298..4146)
                     /locus_tag="IXT20_001811"
     CDS             complement(3298..4146)
                     /locus_tag="IXT20_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAQ4860249.1"
                     /translation="MKETRQFIWTYIKIIFALFILGVAINMFLGPHHIAAGGVSGLGI
                     LLEAALGFDRAIVILVLNIVMLVLALLFLGKKPFFKVLFGSLVFPLIIAIVPETMITS
                     DRLLSVIFGSAIFALGVAILYKNNSSSGGTTIPPLIFKKYFHLNTSIGLFFTDAFVVS
                     LNLFIFGIEEFLYAILSIAITSIVMTYIETGINRKKSVMVMSEHYLEDIRLRLSAEIG
                     RGLTLLEAKGGYNRKSKEVLLIVVTDHEFSRMKPLIEEIDPSAFVIVSSVAEVMGRGF
                     TYHPIE"
     gene            4535..6301
                     /locus_tag="IXT20_001812"
     CDS             4535..6301
                     /locus_tag="IXT20_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glycosidase"
                     /protein_id="HAQ4860250.1"
                     /translation="MDTAAIYHRPESEYAFLYTNTRFRIRLRTRKNDIKKVYVLCGDP
                     YTITTEKWYQKQRPMKKCLSTNVHDYWEIEVTSETSRLQYAFHVVGEDNTDCFYGDQG
                     IFPYRENVITEPNFYFRIPYFHQIDRFTIPEWVKETVWYQIFPERFANGDKANDPEHT
                     LPWGSKDPDREDFFGGDLQGVMDHLDYLTDLGINGIYFCPIFKATSNHKYDTIDYYEV
                     DPAFGDKKLLKNLIDEAHKRGIRIMFDAVFNHMGVHSPQWQDVLKNGEKSIYKDWFHI
                     RFFPVDSYQMTDLPETAENIPYDTFAFTPFMPKLNTANPEVQKYLLDIATYWIKEFDI
                     DGWRLDVANEVDHHFWKKFREAVTEIKPDIYILGEIWHSSQAWLQGDEFHAVMNYAFT
                     DSIKDYFAKKKITASQMVSGMNHQQMLYRDQVNEGTFNLLDSHDTARILTLCQGNKEL
                     MKSVMAFMFLQKGSPCIYYGTEIGMTGEDDPDCRKCMIWEKEEQDLELFGFIKELVSL
                     RKQLSKIISEGSTQWLIVNDREDKLYFTRKLEGQIIYVYFNQSKEPWVVEQENEVILS
                     QNCQLLEDGKAEIRQYGFLIMC"
     gene            6403..7662
                     /locus_tag="IXT20_001813"
     CDS             6403..7662
                     /locus_tag="IXT20_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAQ4860251.1"
                     /translation="MKKRSVKQLGLGLISLGILGGLAACGNGNSSAEGEADEGKTLTV
                     SVDSGYKDYINEIKNTFEKENDVKIKLVEKDMFDQLESLALDGPAGKGPDVMMAAYDR
                     IGALGQQGHLAEVKLGNEAAYDETDKAQVTYDGKIYGEPAVIETLVLYYNKDLVDTAP
                     STFKDLENLSKDSRFAFESEAGKNTGFLAKWTDFYYSYGLIAGYGGYVFGDDGTNPSD
                     IGLNNAGAVEGISYATDWFKNVWPKGMQDIKSAGDFASQQFMSNKTAAIIDGPWQAQT
                     YKENNINYGVAKIPTLNNGQPYQPFGGGKGWVVSNYAKNKDLSQKWLDYVTNQENQEK
                     FFEMVNEIPANQQARETAKAKNDELTTAVIEQYETAQAMPNIPEMGEVWTGAENLMFD
                     AASGSKTPKESADEAVKTISEAIEQKY"
     gene            7809..9110
                     /locus_tag="IXT20_001814"
     CDS             7809..9110
                     /locus_tag="IXT20_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ4860252.1"
                     /translation="MQQDKTSNINEKKITLCSLIPGMGQFVNGQAFKGIVFFAIFVLF
                     IGQMLLGGFHALVGLVTLGSIPMEDHSLFILIEGTLQFIITIIFLIFYGLNLLDAYRV
                     AKMRQEGKKINRTMKEVIHNIGDEGFPYLLTLPAYLLMIFTIIFPVLVTLFTAFTNYD
                     FNHIPPASLIDWVGAKNFFSIFFLSSYRNTFFAVFSWTVIWTLCATTLQITLGILTAV
                     VAHQKFIKFKRIFGIIFLLPWAVPAFITIMSFSNIFNDSVGAINTQVIPFINQLPFVD
                     ISAISWKTDPNWTKVAIILVQGWLGFPYIYVMVTGILQSISEDLYEAAKIDGANAWQR
                     FSNITLPAVFLVAAPTFITQYTGNFNNFSMIYLFNNGGPGSVGGGAGSTDILISWIYK
                     LTTGTSPQYSMASAITLIISVIVICVSLLVFKKTNAFNMGD"
     gene            9113..9964
                     /locus_tag="IXT20_001815"
     CDS             9113..9964
                     /locus_tag="IXT20_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ4860253.1"
                     /translation="MKKFHSSARFQKRLSHFFTYFFLLILSVIIIYPLLITASTAFKS
                     GNIAAFSLDLSSDWTLGNFTRLFSETLYGTWYKNTLIISVLTMIVQVTVVTLAGYTYS
                     RYRFIGRKSSLMFFLVIQMVPTMAALTAFYVMAFLLEALDKYWFLTMIYIGGGIPMNT
                     WLMKGYFDTVPIDLDESAKLDGAGHFRIFFQIVLPLVKPMLAVQALWAFMGPFGDFML
                     AKFLLRTPENQTVAVGLQTFISDVTNQRVSLFAAGAILIALPICLLFFFLQKNFVTGL
                     TSGGTKG"
     gene            9977..10792
                     /locus_tag="IXT20_001816"
     CDS             9977..10792
                     /locus_tag="IXT20_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrose utilization protein MalA"
                     /protein_id="HAQ4860254.1"
                     /translation="MQTFPLNYFSALRTPTQVFAGRKLLSWPKFFLIFVFLVSLMVMP
                     VTLFYANQIQAIPLEQFLSVHSLIDEQGTQKFSELELSETGLQASQQTIAVTPEILVG
                     VSLSEKQQSDHGTFIDFEKEQWVIQQKDKSGIRRYTMNYSPSFQPDSVRTPEDFQRFL
                     EREFYASNRPTIILSYSLSLGLVLFVMTSLILFGASFFLWMTRKSQLSSIHTFKESAN
                     LMLNVMGIGSMAAAVVGFIHFDFILMLSVQTTITVLLLLWVFVKTRFKDKRVE"
     gene            10943..12109
                     /locus_tag="IXT20_001817"
     CDS             10943..12109
                     /locus_tag="IXT20_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAQ4860255.1"
                     /translation="MLEKTLYNQLFSRSFSLPVEVNYWDGTTKQYGDTDTPPQIKINI
                     HEEIPMKEITSNASLALGEAYMDHRIEIEGSIQALINDAYQQANSFLRGKDYLKWLPK
                     KEKHTKQKNKEDIHAHYDLGNDFYKLWLDPTLTYSCAYFNTPEDTLEQAQINKVHHIL
                     DKLFIKEGETLLDIGCGWGTLMFTATKEYNVKATGITLSEEQYDLITKRIKEEHLEDK
                     CRVLLMDYRELKGETFDHITSVGMFEHVGSENLEGYFKVVKDLLKPKGTALIHGISRQ
                     QGGATNAWINKYIFPGGYVPGVTELVDNITKNDLQLIDLESLRRDYQLTLEHWTKNFH
                     QVQDQVTKEMGDSFYRMWDLYLQACAASFESSNIDVIQYLLVQPGNNDIPMHRA"
     gene            12339..12986
                     /locus_tag="IXT20_001818"
     CDS             12339..12986
                     /locus_tag="IXT20_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HAQ4860256.1"
                     /translation="MKKTVIYTSLFAALFLAACQSNTNQGTSGNTSSSSEQRSSQVSD
                     SSTKASTSSSSQTKSSSEASTQSDIKVSAARAIELFQDKYPDTAITSLELDSDWGSYF
                     YKIEGVDDQKEYEARIDAQNEKMETENPETLDRDEQNGQKKKEDGLDVDKLITIEEAA
                     KIAVDKVGSGTATDWDLDKDLGTTYWEVKVKDGNQSTSVKINSQTGEVLSTEQDD"
     gene            13070..14014
                     /locus_tag="IXT20_001819"
     CDS             13070..14014
                     /locus_tag="IXT20_001819"
                     /EC_number="1.3.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate oxidase"
                     /protein_id="HAQ4860257.1"
                     /translation="MSLETTFANHIFANPLMNASGVHCMTTQELDELAHSEAGAFITK
                     SCTINERKGNPEPRYFDVPLGSINSMGLPNLGFSYYLEYALAYEKVQENQNQPLFFSI
                     AGMSVQENLEMLEKIEKSGFNGITELNLSCPNVPGKPQLAYDFEATYETLKEVFSIFS
                     KPLGIKLPPYFDFAHFDQMADILNQFPLTYVNAINSVGNGLYIDTEQEAVVIKPKEGF
                     GGIGGEYIKPTALANVRAFYTRLKPEIQIIGTGGIRTGQDAFEHLLCGASMLQIGTEL
                     HKEGPEIFSRIIKELTQIMSEKGYTSIDEFKGKLRTIS"
     gene            14149..15600
                     /locus_tag="IXT20_001820"
     CDS             14149..15600
                     /locus_tag="IXT20_001820"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HAQ4860258.1"
                     /translation="MNDATELAKELKEKEFSFQELVTDIQKKIKKQNHELNAFVALEP
                     FESNSHELLIPQSEKGPLAGIPFPLKMLGQEKKGWLATSGSRLFETHRASHTSNYVRQ
                     AEAIGLVPFGQTNAPEFGFKNITDPVIYGPARNPWNLECTPGGSSGGAAAAVSSGIVP
                     LAGASDGGGSIRIPASFCGLIGLKPSRGTMPVGPYAWRGWQGAAIDFGLTVSMRDTEA
                     LFYGMRSIHSGAPYQVSPVEWQTHPRKKRLKIAFCVDSPIHSQISEEAVLAVKKAVDF
                     LGKEGHDLTEIPYPLDGRKLITSYYQMNGAETAAMIRSIETGIQRAVRKEELEPISWT
                     LFQYGQKLSAATYIDSLHVWDEAAIVMENLHQEYDLFLSPTTAFTAPKISEDLQSDAI
                     RAQMEHISDCSEVEGLDVVSSMFEESLKITPYTQLANLTGQPAISLPTHLTKENMPLG
                     IQFMAARGREDLLFQIGYLFEANHQFHLPNPWK"
     gene            15621..16457
                     /locus_tag="IXT20_001821"
     CDS             15621..16457
                     /locus_tag="IXT20_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HAQ4860259.1"
                     /translation="MKKKRLFALITSAFFILSACGTAAANQEDNSWSNVEKKKELVVA
                     TSGTLFPASYYNDQNELVGYDVDLAKEVAKRLNVKISFKEYNVDGQITSVSKRESDFA
                     ANDFGITKEREKKFSLSETFKYSFDSMIVRKKDDSGIHSLEDLKGKKAAGEPNTSYMR
                     LAEKHGAKLVTYDNATNDQYLSDVANGRTDVILNDYYLQKMAVSALPDIPVKILENVY
                     FNENESGFLLKEGNDALKKKIDGVLKEMKEDGTTKKISEKYFQTDVSVKPKETMAESV
                     SE"
     gene            16457..17116
                     /locus_tag="IXT20_001822"
     CDS             16457..17116
                     /locus_tag="IXT20_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAQ4860260.1"
                     /translation="MYFPLIDFSLMWTSLPFVLQGLVYTLGIGFVSFLLGNLVGLLLT
                     VLGLLDWLPLNVFIRFYLSFFRGIPALVLLFLLYFGLPYQLSALTASVICFTITSSAF
                     IGEIYRGSLAGVSSGQWDAGLASGFSFFGVLKYIVLPQAFRISVPALSNVAMDLVKGT
                     SLAATITVPEIFQKAKIIGGRTFDYFSLYILVALIYWVICIGIEKVQQYLEKRSMHQS
                     I"
     gene            17242..17931
                     /locus_tag="IXT20_001823"
     CDS             17242..17931
                     /locus_tag="IXT20_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4860261.1"
                     /translation="MKTPLTIEIESSLYQYCIEQGGLVVEEVTMPDDQGIVDTLSCLT
                     KFDGSREWRCYELKVTKADFRSTAKLSFVGHYNYFVLPSKLYEEVKTEIPTGIGVLVY
                     RPYTVEEEMPASGTFLIAKKAAKKELAVPEEALTNRFMASLFREVRKAKQMERGVKYF
                     PTDQLYKELKRRSENQDPFSNERYYERFLEEIASQAINELQEEVIALQHDYEYLRQQQ
                     QIRRLPTEPLE"
     gene            17937..18893
                     /locus_tag="IXT20_001824"
     CDS             17937..18893
                     /locus_tag="IXT20_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sce7725 family protein"
                     /protein_id="HAQ4860262.1"
                     /translation="MYYPYLRGKQFDLLALKEALSRGLLSNKIQPVIEPVRDSATLKN
                     VIELFQKKHHPIAVIQNPQVGQFKLFDQHVHSWTVKENSSVVPAQILTPENLSEVLDS
                     PPAFLVFDGQHYPKDTEVWKQLAGVDSKFLIPDTSRFRIWLPENKIVIRDSFQTRKHV
                     ESYADKTDDFFSDDYLFFHADGYIGFSDFTIEGSRYFDKGFPSRALAIHLTYIDAYGN
                     IRVKHFVSDSNDSAKDQALKFLEAGDKMKKWIMRHHHQLLITSGMIELLALYQQQKFP
                     GLGVLKKWSLMHHLELISHLLDHPKDWLKYYSKVQELYEVIQ"
     gene            18937..19449
                     /locus_tag="IXT20_001825"
     CDS             18937..19449
                     /locus_tag="IXT20_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ4860263.1"
                     /translation="MYIRQTRKEEVEEVVEIINAAADFIASQGSPQWQNGQKPTKETI
                     LNDIERKESYVLIVEDQIAGTAALVSSVDPVYTKIDGSWKESTGPYLSIHRVALAEST
                     RGKKLAKPFLEHLLTIAASKNIQDIRIDTYPANEPMEKTIYSAGFTYQGMIEFPFEHG
                     ERKAYQIIIN"
     gene            complement(19705..>20008)
                     /locus_tag="IXT20_001826"
     CDS             complement(19705..>20008)
                     /locus_tag="IXT20_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HAQ4860264.1"
                     /translation="KKKLTVGNCYAVLTANGFWHLYEYFGTVVRPIFITISKETLQSR
                     LKKRGDSSEQISARLAVYVEDLAQLKRLKAIPSLLVVSNDGAFQEAADKIKKGLLI"
BASE COUNT         6685 a         3570 c         3902 g         5851 t
ORIGIN      
        1 tccagaagaa aaaccagatg tgactccaga accagatacg gacgcaagag accaaggaat
       61 accagaaaaa ataaacaaaa aaacaattca agaggatgga aaaaaagaga gtaaaaaatc
      121 aaatctagca attctaaaaa taaatgaaga gcagctgaat aaaaaatcac gaatatttga
      181 tagcgcccaa tcagctgaaa ccttaaaaag tagcaaggat acaacctttg ctgtcctgaa
      241 acgaaaaata aacagctacc aaaatccgga gagtcacaaa acaaagttat actgtggagc
      301 ggaatcattc tattaagtat agcaacaatg ctttctgcaa aaagatttaa acaaaacaga
      361 tctctctaac atttgaataa tgtctgtgaa cgcatttttc aaataaaaag aaatatccag
      421 aaaatgaccc caaaatacca gattgaaatc tgatatttgg ggtcgttcat actttatctt
      481 tcttcatatt tattttaagg gatatctgtt tcttttggaa gatggttcgg gatctttttc
      541 actttcaata catctaaaaa gaaggtaaag atcggaatgc cgacaatcaa gccccaaacg
      601 ccaaagaacc gttcgctaac aagtaagacc acaaatgtat aaaaaatcgg cagttctgtt
      661 ttgctggaca tcaacttcgg attcagcaca taagactcaa ttagatggac aatcaaaatc
      721 gtaatcagga tataaacaac atctcggatg cctccttggg aataagctat gaaagataga
      781 ggaatacaag aaatgataac acctgctact gggatcagac ttaaaatgaa aatcatgatc
      841 gctaaactca gcagctgatg aaagccaaat acagccaagg cgattgtagt gagcaacgta
      901 tttaaaagag cgacaacaaa ttgagcttct aataccaaac caaatgtatt gacaaactta
      961 tcggcaaaat agtaaatatc ttgaaagaac caagaaaatt cactttttaa aaacaatctt
     1021 gagaatacaa ttgttttttt cttttcaatc ataaaaaaga aacttaagat gaatgacata
     1081 gcaaacgcca ctgccatttt tcctatatcc tgtaaatagc caagcgcaat cgaagcgcca
     1141 ttttgaagct gttcaaaaaa atcgtttcgc tcaatataag tatggatata ttgtaaaaca
     1201 ggatctgcat cttttggctg atgctcgtaa aaattaataa cggactggat catgtcaaat
     1261 gtttgatgaa taagcatcgg gatatacttt gtgaatgcca gatagataag gtaaacgatc
     1321 agtgtataaa tcatgatagc taaaacacct gtagggattc ggacaaatcg ctgaatgatt
     1381 tgaacaagat gaatcatcaa ataagtaaag ataaatgtga gcaaaatggt tgtaatcata
     1441 cttctaacta agaaaagaac aaaaacgaca aataataaaa cagtgagacg gcgtattttt
     1501 tcattcgcca aaaaacgctg ataaacggtc aaatcgacaa cctccaaata acaaaaaata
     1561 aacacgttat tcattatatg atggaatatg aaaataagaa agagcaaaaa gaaaaaccgc
     1621 taaatgacgt tatcttatga gttggttttc ctcataaaat cgttcatcta gcagtttcag
     1681 ccctttttct ggtcttgatg aaaattatct agcatttttc gggtcagatg ttgcagtgta
     1741 tccaattctt cctgttcaag tggaagataa gacttgatct tttgtggaat accttctgct
     1801 tgtttttgca acgcagcacc ttgagcagtc agcgtaacat ttacgattct ttcatcaccc
     1861 gtttcacgct gacgattaat ataatttttt tctgctaact tttttagtaa gggagtcagc
     1921 gtaccagaat ccagacacaa atcatttcct agctctttca ctgaagtctt tttcttttcc
     1981 cataaaacta acatcacaag atactgggta taggtaagat tcagcggctt gaggagggga
     2041 gtgtagtgtc taatgatttc tttagaggcg gcatataaag gaaaacatac ttgattaact
     2101 aaatatagat cttccatttt tccacctccg ctgttttact atagtgtatc atagcggatt
     2161 tacacaaaaa agtctattca ctaactgctt tcagagtaac ttctatattt ccagctgtag
     2221 cttttgaata cggacacact ttatgcgctg cctctaataa ttcttgcgtt tcttccagag
     2281 aaagaccttc gatagatcct tcaatctcta cacctaagac aaagctcgtt tcactttctg
     2341 aataaaggga aacttgtgca ctgacagtag aagccccaga gatatttttt tgctgtttga
     2401 tcaattctaa tgcactgtta aaacatgcac tgaaaccagc tgcaaacaat tgttcagggt
     2461 tggttgcatt ttcgacgcgt ttaccaggag ctgcaatatt caattcgaat gtacgatcgg
     2521 gattatgcac ttctccagag cgtccgcctg tgttgatcat tttcgtagaa tacatttttt
     2581 tcataaaatt accaccttta attatatttt atacaattga attttacaca atataattgt
     2641 ggaaagcaaa gaaatggatt ggcataagat aaataagaga aagcacatga aacagacgcg
     2701 ctttctcgaa tagcaaacat atacaaaaaa tatcagagac aaaaaataat ctcatcataa
     2761 acattacata tatttctcaa aaagttgttt tctttcttca aaaattactt cacgatggac
     2821 ctgatgaagc atccataaat aaccaaaagg atctctgaac attgagttag tcacaccaaa
     2881 gtccatcatt tctgtcattt cttgaatctc tacacagcca tttcgtaatg ctttattatg
     2941 tgtgtcctcg atatcagaga cgatgatatt gaaccagatc gtatttggat gttcagcagt
     3001 aggagcagtc aaaccaagag acgtgttttc atccagcata tggaatctcg taccatacaa
     3061 ggtaaaaatg acttcattat tgcctctagg gtaatctgtt gcctcaatca gttgtatttc
     3121 atcaaagatt gtttgataca gttctagtgc tttcaaacta tcatttaata caaaatcgat
     3181 ttccgttcct ttatgcataa agatctcctc tccttttcag tcgaacagtt actagattct
     3241 tttattatac cagctaaaga aaacgcattc aaaaaaatag cggatttact taccaactca
     3301 ttcgattgga tgataagtaa atccgcgtcc cattacttct gctacgctgc tgacaatgac
     3361 aaaggcactt ggatcgattt cttctatcaa tggtttcatt cgggaaaatt catgatcagt
     3421 cacaacgatc aataaaacct cttttgactt tcgattatac ccgccttttg cctctagcag
     3481 ggtaaggcca cgtccaatct cagcacttaa gcgaaggcgg atgtcttcca aataatgttc
     3541 actcatgacc ataacagatt tctttcgatt gatacctgtt tcgatataag tcatgacaat
     3601 tgaagtgatt gcaatagata aaatagcata aagaaactct tctattccaa aaataaacag
     3661 attaagagaa acaacgaatg cgtctgtaaa aaataagcca atcgaagtat tcaagtggaa
     3721 atatttttta aaaatgaggg gagggatcgt cgtaccgccg ctagaagagt tgtttttata
     3781 taaaatcgca actcctaaag caaaaatcgc acttccaaaa atgacagaca acaaccgatc
     3841 tgaagtaatc attgtttctg gaactattgc aatgattaaa ggaaaaacca agcttccgaa
     3901 aagtacttta aaaaagggtt ttttacctaa aaatagaaga gctagtacca acataacgat
     3961 attcaacact aaaataacta tagctcggtc aaagcctaaa gcagcttcca gtaatattcc
     4021 tagaccgctg acacctccag cagcaatatg atgaggccct aaaaacatat tgatagcgac
     4081 tcctaatatg aagagggcaa aaataatctt aatgtatgtc catataaatt gtctcgtttc
     4141 tttcatttat tcacttcctt aattcattaa taatactctt atcttattat aacttacttt
     4201 tcaccttaag ttaactttaa ttaataaaaa aattaggcta gtctaaatta ttatgtttca
     4261 ttcttctaga atatacttct acgccaaaaa ctgttaaaaa acttttactt tagaaaattg
     4321 gagaaattga tcgcttaaga taaattcagg acactaaata gtacaggaag tagactaaaa
     4381 cttttaaaag ttttaagtct atctatttgg caagtttaaa aaataaaaat tcggtatcaa
     4441 aaaactgtta ccgataacat ttttgaaagc gttgtctttt gcttctctat cacttatagt
     4501 gatgacgtca tattaaaaca caaggaggaa atcaatggat acagctgcaa tttatcatcg
     4561 accggaaagt gaatatgcat ttctatatac gaatacaaga tttcgtattc ggcttcgtac
     4621 taggaaaaat gatatcaaaa aagtatatgt gctatgtgga gatccatata ccataacaac
     4681 tgaaaaatgg tatcaaaaac aacgaccaat gaaaaaatgt ctaagtacca atgttcatga
     4741 ttattgggaa attgaagtga cgagcgaaac aagtcgctta cagtatgcgt tccatgttgt
     4801 tggggaagat aacacggact gtttttacgg tgatcaagga atttttcctt atcgagaaaa
     4861 tgtgattact gaaccaaatt tttattttcg aataccttat tttcatcaaa tcgatcgatt
     4921 cactattcca gaatgggtaa aagaaacagt atggtatcaa atttttcccg aaagatttgc
     4981 aaacggggac aaagcaaatg accccgaaca cactttacct tggggaagta aagatccaga
     5041 ccgagaagac tttttcgggg gagatttgca aggtgtgatg gatcatttgg attacttgac
     5101 ggaccttggc atcaatggca tctatttttg tcccatattt aaagccacat ccaatcataa
     5161 atacgatacg attgattatt atgaggtgga tcccgctttt ggcgataaaa aacttttaaa
     5221 aaacttgatt gatgaagcac ataaaagagg gatacgtatc atgttcgatg ctgtattcaa
     5281 tcatatgggt gtccattcac cacagtggca agacgttctc aaaaacggag aaaaatcgat
     5341 ctataaagac tggttccaca tccgtttttt cccagtagat tcttatcaga tgactgactt
     5401 accagaaact gctgaaaata tcccatatga tacttttgct tttactccat tcatgccgaa
     5461 actaaataca gcaaatccag aagtgcaaaa atatttgctg gatatcgcaa cttattggat
     5521 aaaagaattc gatattgatg gatggcgtct tgatgttgct aatgaagtcg atcatcattt
     5581 ttggaaaaaa ttcagagaag cagttacaga aatcaaacca gacatctata tccttggaga
     5641 gatttggcat tcctctcaag cttggctgca aggggatgaa ttccatgcag tgatgaatta
     5701 tgcatttaca gattcgatta aagattattt tgcaaaaaag aaaatcacag cttcacaaat
     5761 ggtcagtgga atgaatcatc agcaaatgct ttacagagat caggtgaacg aaggaacatt
     5821 caacttatta gattctcatg atactgccag gatcttgacc ctttgccaag gtaacaaaga
     5881 attgatgaag tctgtgatgg cctttatgtt tcttcaaaaa gggtctcctt gtatctatta
     5941 cgggacagaa atagggatga ctggtgaaga tgacccggat tgccgcaaat gtatgatatg
     6001 ggagaaggag gaacaagatc tagagctatt cggttttata aaagaacttg tttctctaag
     6061 gaaacagttg tctaagatta tttctgaagg ttctactcag tggctaatag taaatgatcg
     6121 tgaagataag ctctatttca cacggaaact agagggacag atcatctatg tttactttaa
     6181 tcagtcaaaa gaaccttggg tagtagaaca ggaaaatgag gtgattctta gtcaaaattg
     6241 tcagttgtta gaagacggga aagcagagat acgacaatac ggatttttga tcatgtgttg
     6301 aaaactgtta ccggtaacaa caagaaagcg tttgcaaatg aagatttgtg cccattataa
     6361 taagggtgta acagcacgtt gaatacaaag gaggaaatga agatgaaaaa aagaagtgtg
     6421 aaacagttgg gtttagggtt aatctcttta ggaatcttag gcggattagc ggcttgtggc
     6481 aatggtaact cttcagcaga aggggaagcc gatgaaggaa aaacgttaac agtttccgtt
     6541 gattctggat ataaagatta tataaacgaa ataaaaaaca ctttcgaaaa agaaaacgat
     6601 gtgaaaatca agctagtaga aaaggacatg ttcgatcagt tggaatcatt agcgttagat
     6661 gggcctgctg gaaagggacc tgacgttatg atggcagcat atgaccggat cggtgcgtta
     6721 ggacagcaag gacatctggc ggaagtaaaa ctgggaaatg aagcagctta cgatgaaaca
     6781 gacaaagccc aagtgactta tgatggaaaa atctacggag aacctgcagt aatcgagacc
     6841 cttgttttgt actataacaa agatttagtc gatacagcac catcaacgtt taaagatttg
     6901 gagaatctat ccaaagacag tcgttttgct tttgagtcag aagcaggaaa aaatacagga
     6961 tttttagcta aatggactga cttttattat tcctatggtt taattgcagg ttacggtggc
     7021 tatgtatttg gtgatgatgg cacaaatcct tcagatatcg gtttgaataa tgcaggtgct
     7081 gtagaaggaa tctcttatgc aacagattgg ttcaaaaatg tttggccaaa gggaatgcaa
     7141 gacataaaaa gtgcaggaga cttcgccagc cagcaattca tgtccaataa aactgcagca
     7201 atcattgacg gtccatggca agcgcaaaca tacaaagaaa acaatatcaa ttatggtgta
     7261 gccaaaattc ctactctaaa taatggacag ccttatcaac cgtttggcgg aggaaaagga
     7321 tgggtagtca gcaattatgc taaaaacaaa gacctctctc aaaaatggct tgattatgta
     7381 acgaatcagg aaaatcaaga aaaattcttt gaaatggtaa atgaaatccc tgcaaaccaa
     7441 caagctaggg aaacagcaaa ggctaaaaac gatgaattaa caacagctgt gattgaacaa
     7501 tatgaaacag ctcaagcgat gccaaacatc ccagaaatgg gcgaagtttg gacaggtgca
     7561 gaaaacttga tgttcgatgc agcatcaggt tcaaaaacac caaaagaatc cgcagatgaa
     7621 gcagtaaaaa caatcagtga agccatagaa caaaaatact gattaaaatg ataaccgtcc
     7681 atttttataa aaaatggaac aggtgcttca gataaaagtg gcaagagcga gatggaaaat
     7741 tatcttgctc ttgttgccat tatcttaaaa gcagtttgcg gtttagcaag tttagagggg
     7801 gcattcagat gcagcaagat aaaacaagca atatcaacga aaaaaaaatc acgctttgtt
     7861 cacttattcc agggatgggg cagtttgtaa atggccaagc ttttaaaggt atcgtttttt
     7921 tcgccatatt cgtcctattc attggtcaga tgcttctggg cggatttcat gcattggtag
     7981 gattggttac tttgggaagt ataccaatgg aagatcattc attatttatt ttgattgaag
     8041 gcactttgca attcatcatt acaatcattt tcttgatttt ttacggtttg aatcttttag
     8101 acgcttatcg agtagcgaag atgcgtcaag agggcaaaaa aatcaatcgg acgatgaagg
     8161 aagtcataca taatataggt gatgaaggtt ttccttatct attgacactt cccgcttatc
     8221 ttctcatgat ttttacgatc attttcccag tcctagttac attgttcact gcatttacta
     8281 attatgactt caatcatatt ccaccagcca gtttaattga ttgggtaggg gctaaaaact
     8341 tttttagtat cttcttttta agctcatatc gtaatacttt ttttgcagtc ttttcgtgga
     8401 ccgtgatttg gactttatgt gcaacaactt tgcaaatcac ccttggtatt ctcacagcgg
     8461 ttgttgctca ccagaaattt attaaattca aacgtatttt cggtatcatt ttcttattac
     8521 catgggcggt cccagctttt attactatca tgagtttttc gaatatattc aacgatagcg
     8581 taggtgccat caatactcaa gtgattcctt tcataaacca gcttcctttt gtagatatca
     8641 gtgcaatctc ttggaaaacg gacccgaact ggacgaaagt agcaattatc ttagtgcagg
     8701 gctggttagg atttccctat atctatgtga tggttacagg aatcttacaa tcgatttcag
     8761 aagacttata tgaagcagca aaaatcgacg gagcaaatgc atggcagcgc ttttcaaaca
     8821 tcacgcttcc tgctgtcttc ctagtggcag cgccaacatt tattacacag tatacgggga
     8881 atttcaataa tttctccatg atctatctgt ttaacaatgg cggtcccgga agcgtaggcg
     8941 gaggagctgg ttctactgat attcttattt cctggatcta taaacttacg actggaactt
     9001 ctccgcagta ttctatggct tcagcaatca cgttgattat ctccgtcatc gttatctgtg
     9061 tgtcattgct ggtatttaaa aagacaaatg cgttcaatat gggggattaa caatgaaaaa
     9121 atttcattcg agtgctcgat tccaaaaacg actttctcac ttttttactt acttttttct
     9181 tttaatactg tcagtcatta tcatctatcc gcttttgatc acagcaagta ctgccttcaa
     9241 gtcaggaaat attgcagctt ttagtttaga tctttcaagt gactggactt taggaaactt
     9301 tactcggcta ttcagtgaaa cactttacgg gacttggtat aaaaatacat tgatcatttc
     9361 tgtcctaaca atgatcgtac aagtgactgt agtaactttg gcgggctata cttatagtcg
     9421 ctatcgtttt atcggccgca aatcaagttt gatgttcttt ttagtgattc agatggtacc
     9481 gactatggca gcattgactg ccttttatgt catggctttc ttattagaag cgctcgataa
     9541 gtactggttc ttgacaatga tctatatagg cggtggaatt ccaatgaata cttggctgat
     9601 gaaaggatac tttgacactg taccaataga tttagatgaa tctgctaaac ttgatggggc
     9661 agggcatttc aggatttttt tccagattgt attaccgctt gtcaaaccga tgctcgccgt
     9721 tcaagcatta tgggctttta tgggaccttt tggggatttc atgttagcta agttcctgct
     9781 tcgaacacct gaaaatcaga ctgttgctgt cggcttacaa acatttattt ctgatgtgac
     9841 aaaccaaaga gtctctcttt ttgctgctgg ggcaattttg attgcactac cgatttgtct
     9901 attattcttc tttttacaaa agaactttgt taccggcctg acatctggcg gaacaaaagg
     9961 ataggagcag agtatcatgc aaacatttcc gttaaactat ttttccgcat tacgtacgcc
    10021 aactcaagtg tttgcgggac gcaaactatt atcatggcct aaatttttct tgatatttgt
    10081 atttttagtt tccttgatgg tcatgcctgt gactcttttt tatgccaatc agatccaagc
    10141 gattccatta gaacaatttc tatccgttca ttcgctgatc gatgaacaag gaacccaaaa
    10201 attttctgaa cttgaattat ctgaaactgg gttacaagca tcgcaacaaa cgatagctgt
    10261 gacccctgaa attttggtag gtgtgtcttt atctgagaaa caacaatccg atcatggaac
    10321 ttttattgat tttgagaagg aacaatgggt gatccaacaa aaggataaat ctggtatacg
    10381 ccgctatacg atgaattata gcccatcttt ccaaccagat tctgtacgta cgcccgaaga
    10441 tttccaacga tttttagaaa gagaatttta tgccagtaat cgtccaacaa tcattttatc
    10501 ttatagttta agtcttggtc ttgtcctatt tgtcatgacg agtctcattt tattcggggc
    10561 ttcctttttc ttgtggatga caagaaaaag ccaactttca tccattcata cattcaaaga
    10621 gagtgcaaat ttgatgctga atgttatggg aataggttcc atggcagcag cagttgtagg
    10681 gtttatccac tttgacttta tcttgatgct aagtgtccaa acaacgataa ctgtcttgct
    10741 gcttttatgg gtttttgtca aaacaagatt caaagataag cgtgtcgaat gacaagttta
    10801 agcgatttat gcgacaatga atgtgaaaaa aatgaaaaat aaagtagttt ttgaaaaatt
    10861 tctgaaaaaa ttaaacttct tagttgattc ttttcttttt tagtattaac gtaatagggt
    10921 agttttttta aggaggaaaa tcatgttaga gaaaacttta tacaatcaat tattttcacg
    10981 ttctttctcg ctccctgtcg aagtaaatta ttgggacggt acaacgaaac aatacggaga
    11041 tacggacact cctccccaaa tcaaaatcaa tatccatgaa gaaatcccaa tgaaagagat
    11101 tacaagtaac gcttctttgg cactaggaga agcctatatg gatcatcgaa tcgagatcga
    11161 gggaagtatc caagcattga tcaacgatgc ttatcagcaa gcaaacagct ttttacgagg
    11221 aaaagattat ctaaaatggc tgccaaagaa agaaaaacat acgaaacaaa aaaacaaaga
    11281 agatatccat gcgcattatg acttaggtaa cgacttttat aaactttggc tagatccaac
    11341 tttgacttat tcttgtgctt atttcaatac acctgaagat acgttggaac aagcacaaat
    11401 caataaagtc catcatatct tagacaaact ctttatcaaa gaaggcgaaa cgttgcttga
    11461 tatcggttgt ggctggggaa cattgatgtt cactgctacc aaagagtata atgtcaaggc
    11521 aactgggatc acactaagtg aagaacaata cgatttgatt accaaaagaa taaaagaaga
    11581 acatttagaa gataaatgcc gtgtattatt aatggattac cgcgaactaa aaggcgaaac
    11641 gtttgatcat atcacaagtg taggtatgtt cgaacatgta ggttctgaaa atcttgaagg
    11701 ttatttcaaa gtggtaaaag atttattgaa accaaaagga acagctttga tccatggaat
    11761 cagccgacaa caaggcggtg cgacaaatgc ttggatcaat aaatatattt tccctggcgg
    11821 ttatgtccct ggtgttactg aattagttga taatattaca aaaaacgatt tgcaactgat
    11881 tgatttggaa agcttacgtc gtgactacca attgacatta gaacattgga caaagaattt
    11941 tcatcaagtc caagatcaag tcacgaaaga aatgggtgat tccttctatc gaatgtggga
    12001 tctatattta caagcttgtg cagcttcatt tgaatcaagt aatatcgatg tgattcaata
    12061 tttgcttgtt caacctggaa acaatgacat cccaatgcat cgtgcataat tgaggcaaaa
    12121 gtactgttta aagactattt aaaaagaagc taagttttat gatgctccag aagtgaactt
    12181 ttttgtctaa cttctaggat tgtctctaaa gcttagtttc tttttcttta tattttcata
    12241 ataaaagttt ggttctttaa tttttaaaca tgacagaaaa ttttttcttc gttatacttg
    12301 taatagaata ctattttata acaaagggga tcgaatcaat gaaaaaaact gttatttata
    12361 ctagtttatt tgcagctttg tttttagctg cttgtcaatc aaataccaat caaggcacat
    12421 ctgggaatac gtcttccagt agcgaacagc gctcttcaca ggtatcggac tcctcaacaa
    12481 aagcaagcac atcttcgtct agccagacaa aaagttctag tgaagcaagt actcagtcag
    12541 atatcaaagt gtctgcagct cgtgcgatcg aattgttcca agataaatac ccagatactg
    12601 caattactag tttggaatta gatagtgatt ggggatctta cttctacaag atagaaggtg
    12661 tcgatgatca aaaagaatac gaagcaagaa ttgatgctca aaatgaaaaa atggagacag
    12721 agaatccaga aactctagat cgagatgaac aaaatggtca aaagaaaaaa gaagatggtc
    12781 tggatgttga taaattgatc acgattgagg aagcagcaaa aatcgccgtc gacaaagtag
    12841 gaagcggtac ggcaacagat tgggatttgg ataaagattt aggtactact tattgggaag
    12901 tcaaagtaaa agacggcaat caaagtacat cagtcaagat caatagccaa acaggcgaag
    12961 tactgtcaac tgaacaagat gattaagaat ttcttcggaa aacaagagtt gatttttctt
    13021 ttagtcatga tacactagac aaaataactt gtttgaagga gcgatttact tgagtttaga
    13081 aacgaccttt gccaatcata tctttgccaa tcctttgatg aatgcatctg gtgtccactg
    13141 catgactacc caggaattag atgaactggc tcattcagaa gcaggagcat ttatcactaa
    13201 gagctgtacg atcaatgaac gaaaaggaaa tcccgaacca cgctattttg atgttccttt
    13261 agggagtatc aattctatgg gacttccaaa cttaggtttt tcttattatc tagagtatgc
    13321 tcttgcgtat gaaaaagtgc aggaaaacca aaatcaaccg ttattttttt ccattgccgg
    13381 gatgagtgtc caggaaaact tggaaatgct tgagaaaata gaaaaaagtg ggtttaatgg
    13441 gatcactgaa ttaaatttat cttgtcccaa tgtgcctgga aaaccacaac tagcttacga
    13501 ttttgaggcc acttatgaaa cactgaaaga agtattcagc atcttttcta agccgctagg
    13561 aatcaaattg cctccttatt ttgattttgc tcattttgat cagatggctg atatcttgaa
    13621 tcagtttcca ttgacctatg tcaatgctat caacagtgtg ggcaatggtt tgtatatcga
    13681 tacagaacaa gaagcagtag tcatcaaacc aaaagaaggg ttcggcggca ttggcggaga
    13741 gtatatcaag ccgactgctt tggcgaatgt tcgcgctttt tatacacgtt tgaaaccaga
    13801 aatccaaatc attggtacag gtgggattcg gacaggacaa gatgcatttg aacatctgct
    13861 atgtggcgca agcatgttac agattggtac cgagcttcat aaagaagggc cggaaatttt
    13921 ttctagaatc ataaaagaat taacgcagat tatgtcagaa aaaggatata cgtcaattga
    13981 cgaatttaaa ggcaaattac gcaccatttc atagcaaaga gacgaaggtc atgattgatc
    14041 ttcgtctttt tttataaaaa acactcgtct gttcattcct gtgcaagcgt actcattgta
    14101 tgttagacga acttgtggga aaataacaga ggcaaaaagg aggataatat gaatgatgca
    14161 acagaattag caaaagagct taaagaaaaa gaatttagtt ttcaagaact agttacggat
    14221 atacagaaaa aaataaagaa gcaaaatcat gaattgaatg cgtttgtcgc gcttgaacca
    14281 ttcgagagta attcccacga gctattgatt ccccagtcag aaaaagggcc attggctggt
    14341 atcccatttc ctttaaaaat gctcggtcaa gaaaaaaagg gatggctagc tacttcagga
    14401 tctcgtttat ttgaaacaca ccgagcttct catacatcta attatgtgcg gcaagcagaa
    14461 gcgataggtc tagttccttt tggacaaacc aatgcgccag agtttggttt caaaaatatt
    14521 acagacccag tcatctatgg cccagcaaga aacccttgga accttgaatg tacaccgggc
    14581 ggatcaagtg gaggagctgc tgctgcagtt tcatcaggaa tcgttccttt agctggtgct
    14641 agtgatggcg gtggttctat tagaataccc gcttcgtttt gcggattgat tgggctgaaa
    14701 ccttcgcgag ggacgatgcc agttggtcca tatgcttgga gaggatggca aggagctgct
    14761 attgatttcg gcttgacagt ttctatgaga gacacggagg ctttatttta tgggatgaga
    14821 agtatccatt ccggtgcgcc atatcaagta tcgccagttg agtggcaaac acatccaaga
    14881 aaaaaacggt tgaagatagc tttttgtgtt gattcaccga tccactcaca gattagtgaa
    14941 gaggcagtac ttgctgtaaa aaaagctgta gatttccttg gaaaagaagg gcatgatttg
    15001 acagagattc cttacccact agatggtcgt aaactgatca cgtcttatta tcagatgaat
    15061 ggcgcagaaa cagcagcaat gatccgctcg attgaaacag gcatccagcg agctgtcaga
    15121 aaagaagaac ttgaacccat ttcttggact ttgttccaat atggtcagaa gctctctgcc
    15181 gctacttaca tcgattctct gcatgtatgg gatgaagcag caattgtcat ggaaaaccta
    15241 catcaagaat acgatctatt cctttcacca acgactgcat ttaccgcccc taaaatcagc
    15301 gaagatttgc aaagtgatgc gataagagca cagatggaac acatcagtga ttgttctgaa
    15361 gtagaaggtt tagacgttgt ttcttccatg tttgaggaga gcttgaagat cacaccttat
    15421 acacagttgg ctaatctgac tggacaacca gcaatcagct tgcctactca tttgacaaaa
    15481 gaaaacatgc ctctaggaat ccaatttatg gctgccagag gaagagaaga tttgctattt
    15541 caaatcggct atctatttga agcaaatcat cagttccatt taccaaatcc atggaaatag
    15601 cagaaacaag gagaagaaga atgaagaaaa aaagattgtt tgctctaata actagtgcct
    15661 tttttatttt aagcgcttgc ggtacagctg ccgctaatca ggaagataac agctggagta
    15721 atgtagaaaa aaagaaagaa ttagtagtag cgacttctgg cacactgttt cccgcatctt
    15781 attataatga tcaaaatgaa ttagttggtt atgatgtaga tttggcaaaa gaggtggcta
    15841 aacggttaaa tgtcaaaatc tcctttaaag aatacaacgt ggatggacaa atcacttctg
    15901 tttcaaaaag agaatcagat tttgctgcga acgattttgg tatcacaaaa gaaagagaaa
    15961 aaaaattctc cctttcagag actttcaaat attcatttga cagtatgatc gtacgaaaaa
    16021 aagatgattc aggaatccac tcacttgaag atcttaaagg gaaaaaagca gctggcgaac
    16081 ccaatactag ctatatgcgt ttagcggaaa aacacggagc taaattagtc acatatgaca
    16141 atgccacgaa tgatcagtat ttaagcgatg tagcaaatgg tcgaacagat gttattctaa
    16201 atgattatta tctgcagaaa atggccgtat ctgctttacc agatatccct gtaaaaatac
    16261 tggagaacgt ttatttcaat gaaaacgaaa gtggtttttt attgaaagaa ggaaatgatg
    16321 ctctgaaaaa gaaaatcgat ggcgtattaa aagagatgaa agaagatggg acgacaaaga
    16381 aaatatctga aaaatatttt caaactgatg tttcagtcaa accgaaagaa acgatggctg
    16441 agtcggtgag tgagtgatgt actttcctct aattgatttt tccctcatgt ggacttcact
    16501 tcctttcgta ctgcaaggct tggtatacac attagggatt ggcttcgtgt cgtttctttt
    16561 aggaaatctg gtagggttgc ttttgacagt tttaggtctt ttggattggc tgcctctgaa
    16621 tgtatttatt cgtttttatt tatcgttctt tagaggcatt cccgcactag tcctgctgtt
    16681 tctgttatat tttgggttac cttatcaatt aagtgcctta acggcttcag tcatctgctt
    16741 tacgattacc agcagtgcat ttattggtga aatctatcga ggatcactag caggcgtttc
    16801 ttcaggtcag tgggacgctg gtttagcttc tggtttttct ttctttgggg tattgaaata
    16861 tatcgttttg ccgcaagctt ttcgaatctc agtaccggca ctcagtaacg tagctatgga
    16921 tctagtgaaa ggtacttcgt tagcagcaac aatcacagtg ccagaaatat ttcaaaaagc
    16981 aaagatcata ggcggtcgga cattcgatta tttttctctc tatattttag ttgcactgat
    17041 ttattgggtc atttgtatcg ggatcgaaaa agtgcaacag tatttagaaa aacgttctat
    17101 gcatcagtca atctgaaaaa tagaaatggt gctagcgtgg ctggcaccat ttctattttt
    17161 tgtttcttgt atattctgcc tagtatttta aaaccatttc atgtaaaatg taaatcgaaa
    17221 atatgttcgg aggtagaaaa catgaagacc ccgctaacca tcgaaataga atcctcgtta
    17281 taccaatatt gcatcgagca aggcggcttg gttgtagaag aagtcacgat gcctgatgac
    17341 caaggaattg tagatacgct tagctgcttg acaaaatttg atggaagccg agaatggcga
    17401 tgctatgagc tgaaagtgac aaaagcagat tttcgctcta cagccaaact gtcatttgtt
    17461 ggacattata attattttgt tttaccaagt aaactatatg aagaagtaaa aactgaaatc
    17521 cctacaggaa taggtgtttt agtgtatcgt ccgtacacgg ttgaagagga gatgcctgcc
    17581 tctggtacat ttctgattgc gaaaaaagca gcaaagaaag agcttgctgt accggaagaa
    17641 gctttgacaa accgcttcat ggcttcttta ttcagggaag taagaaaagc aaagcaaatg
    17701 gagcgaggcg taaaatattt tccaacagat caattgtaca aagaattgaa aagacgttca
    17761 gaaaatcaag atccattttc taacgaacga tattatgaac ggtttttaga ggaaatagct
    17821 agtcaggcaa tcaatgagtt gcaagaagaa gttatcgcgc ttcagcatga ttatgaatat
    17881 ttgaggcaac aacagcagat ccgtcggttg cctacagagc cattggagtg aaaaatatgt
    17941 actatcctta tttaagagga aaacaatttg acctattagc attaaaagaa gcactgtcgc
    18001 gaggattgct ctccaataaa atccaaccag tgatcgaacc agtacgtgat tccgctacgc
    18061 ttaaaaatgt gatcgaatta tttcaaaaaa agcaccatcc aatcgctgtg atccaaaatc
    18121 cccaagtagg acaattcaaa ttattcgatc agcatgtgca cagttggaca gtcaaagaaa
    18181 atagctctgt cgtccctgca cagatcttaa cgccagaaaa tctcagtgaa gtactggatt
    18241 cacctccagc gtttcttgtt ttcgatggac agcactaccc aaaagataca gaggtgtgga
    18301 aacagttggc aggagttgat tcgaaatttt tgataccaga tactagtcgc tttcgcatct
    18361 ggctgcccga aaacaaaatt gtgatccgtg actcttttca aacgagaaag catgttgaaa
    18421 gttatgcaga taaaacagat gactttttta gtgatgacta tctttttttc cacgcagacg
    18481 gatatattgg attttctgat ttcacgatcg aaggtagccg ttatttcgat aaagggttcc
    18541 ctagccgagc gcttgctatc catctaacgt atattgatgc ttacggcaat atacgcgtga
    18601 aacattttgt ctctgacagt aacgactctg ctaaagacca agcattgaag tttcttgaag
    18661 caggagataa aatgaaaaaa tggatcatgc gtcaccatca tcaactcttg attacttcag
    18721 gaatgattga attattggct ctttaccagc aacaaaaatt ccctggactg ggagtgttga
    18781 aaaagtggtc acttatgcat catttggaac ttattagtca cctgctagat catccaaaag
    18841 attggcttaa atattattca aaagttcagg aactatatga agtgatccaa tgattagata
    18901 atgtaaatga aaaaggaata gaaaagagga aactggatgt atattcggca aacaaggaaa
    18961 gaagaagtag aagaagtggt agaaatcatt aatgccgcag cggatttcat tgcttcgcaa
    19021 ggctctcctc aatggcaaaa cgggcagaaa ccaacaaaag aaacgatttt gaacgatatt
    19081 gaaagaaaag aaagctatgt attaatagtg gaagatcaaa ttgctggaac agctgcatta
    19141 gtctcaagcg tggatcccgt ttacacaaaa atagatggta gttggaaaga atcaactggt
    19201 ccttatttat cgattcatcg tgtcgcttta gcagaaagta ctcgaggaaa aaaactagca
    19261 aaaccgttct tagaacattt gttgacgatt gcagccagca aaaatatcca agatatccgt
    19321 atcgatactt atccagctaa tgaaccgatg gaaaagacca tctattctgc tggattcact
    19381 tatcaaggga tgattgaatt tccttttgaa cacggcgaaa gaaaagcata tcaaataatc
    19441 atcaattgac tataaaatca tctgagcaaa gataaagaca ttaacactac tgaaaaaaac
    19501 aatcatatga tagccaagag gttgtaacag aagtggccag cttcaaaaaa caagaaagaa
    19561 tttccgaaaa tgcttctcaa atttttgtgg aattttagcg tattttacga aatagctgct
    19621 ttctgaaact cccttcatcg agaggtcgtg acatagcttt tgtcacgacc tctcggctta
    19681 tcctacgagt attattcgat acatttatat caataatcct ttttttattt tatctgcagc
    19741 ttcttggaac gctccatcgt ttgacacgac taaaagtgag gggatagctt tcaaacgttt
    19801 caactgtgct aaatcttcta cataaaccgc cagtctggct gaaatctgtt cagatgaatc
    19861 tccacgtttt ttcaggcgcg attgcaatgt ttctttagag atcgtaataa agataggacg
    19921 gactaccgtc ccgaaatact catataaatg ccaaaaacca tttgctgtca aaacggcgta
    19981 acaatttcct actgtcagtt tcttttta
//
LOCUS       DACGLE010000047        19843 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0534
            SAMEA3304449-rid9560453.denovo.051, whole genome shotgun sequence.
ACCESSION   DACGLE010000047 DACGLE010000000
VERSION     DACGLE010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304449
            Sequence Read Archive: ERR985064
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19843)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19843)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702225.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702225.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 20:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,045
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 27 of 67
            Pseudo Genes (incomplete)         :: 25 of 67
            Pseudo Genes (internal stop)      :: 19 of 67
            Pseudo Genes (multiple problems)  :: 4 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19843
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304449-rid9560453.denovo.051"
                     /strain="VREN0534"
                     /db_xref="taxon:1352"
     gene            complement(<1..244)
                     /locus_tag="IXT20_001827"
     CDS             complement(<1..244)
                     /locus_tag="IXT20_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HAQ4860265.1"
                     /translation="MNYCYVFIGPSGSGKTSLAEAIFQPEQKIITYTNRSPRPGEKDQ
                     EDYYFVSDNTFNQMIKGNEFAEWDQYAQHKYGSSKAE"
     gene            complement(333..1514)
                     /locus_tag="IXT20_001828"
     CDS             complement(333..1514)
                     /locus_tag="IXT20_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HAQ4860266.1"
                     /translation="MSYLLGIIGLFVTLGLAFLISSNKKAIKLKPLIVMFVLQLVLGF
                     VLLRTTFGTAVVSVLAKMFDHLLAFAGQGVDFVFAGVANKGSAPFFLSVLMPIVFISA
                     IIGILRYIKILPLFMKAVGLGLSKINGMGKLESYNGVASAILGQSEVFISIKKELPFL
                     SEKRLFTMSVSAMSTVSMSIVGSYMALIDSKYVITALVLNLFGGYILASIVNPYVLEE
                     KEDELIIEENKEQTFFQMLGEYILDGFHVAITVAAMLIGFVALIAMINAIFHGIFGIT
                     FQEILGYIFAPLAFISGIPWKEAVDAGSIMATKLVTNEFVAMTELANGNFHFTERTTA
                     IISVFLVSFANFSSIGIISGAMKGLNEEKGNLVAKHGLKILLTATLVSFLSAIVTGLL
                     V"
     gene            complement(1750..3258)
                     /locus_tag="IXT20_001829"
     CDS             complement(1750..3258)
                     /locus_tag="IXT20_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HAQ4860267.1"
                     /translation="MKKIGFDSEKYIEEQSAYILERVNHYDKLYLEFGGKLVDDKHAK
                     RVLPGFEEDAKIKLLQKLKDQAEILICVYAGDIERNKIRGDYGITYDMDILRLIDELR
                     GYGLLINSVVITRYSGQPATKVFINKLERRNIKVYKHSEIEDYPVNVEKIVSEDGFGK
                     NEYIETTKPIVVVTAPGPGSGKLATSLNQLYHESQKGHAAGYSKFETFPVWNVPLKHP
                     LNIAYEAATVDLKDVNMIDSFHFDAYNKVAVNYNRDVETFPVIKRIIEKITGKESVYQ
                     SPTDMGVNRVGFGIVDDTVVQEASKQEIIRRYFQTECDFKKGLIDEEAVNRIKLIMEE
                     VGLRPEDRNVVVPAHDYADKIQAQSPTKDPSAVIAIELPDEVIITGRTSSLMDASAAV
                     ILNAVKHLAHIADDIPLLSPLVLETIQGLKSKTLHSSLDALSINEVLIALSISAVTNP
                     IAQVAYDKLAELEGAQAHSTVMVNKNDEQTLKKLGIDITCAPVYPSENLYYQ"
     gene            3417..3608
                     /locus_tag="IXT20_001830"
     CDS             3417..3608
                     /locus_tag="IXT20_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4860268.1"
                     /translation="MKKRQKKKNAYKHYIRSIFTGYERMLEDPELEQLTFTYLNEETQ
                     LTRDEHQRIHFTTRDLPSK"
     gene            3811..4728
                     /locus_tag="IXT20_001831"
     CDS             3811..4728
                     /locus_tag="IXT20_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="viral A-type inclusion protein"
                     /protein_id="HAQ4860269.1"
                     /translation="MKEPDTNANYEPDELEETNADQDTGQESSSFYDIKEKASMNLSQ
                     LLEDLQAFEKAVREEDIPEIYRLYNGRLNEELKRSSNENHEIDQLLMRKIHDRFLQEF
                     PFMEQVENISPTMSYYKIGTYYHDRPTVGIDASLPEIFVLPKIDEEWEKYSQPESTDY
                     DKKINELDAKVITAQTEIERLDEQIKEINRQMTDLNDNKGFLNRKKIEEEIQELEKKK
                     QVLSNEKLGWLPYIETPETIQQQKEALKQEARADQLRAAIVEKEQRQIRRYFGSKEGF
                     GQAIHEFLMNYLGNENPNNQSNEGGSEYE"
     gene            4721..5794
                     /locus_tag="IXT20_001832"
     CDS             4721..5794
                     /locus_tag="IXT20_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4860270.1"
                     /translation="MNEHDEAEWTKNEEQTEDHEETNTSLEEQTSNPLDDTEEIPMLE
                     ETKIHEKEKVTPTNTNTAENTHAYEITENPGYLEGKEQDHQDIVSLIETAEAELADLR
                     MRKDLIESDFPQLLESYDRFILNEEPISTIGKTSLIEYFTYELLKPLNDIGLERSNSA
                     YDTWKTRHFSINYTLNGQNELVFSFVLPTIDQRYQVESMSLLNVSPETMEINVQDDRV
                     LSLIRHWSVDRVFSAGQITIFNHKLNQLLAHARTLGFTVNQTLLDNTKPLRLNLQSEF
                     ELTNEVLDDIFIVAMKNPSYDMEKINETTYQVRLDKGQSLTITTNEKDQTVLSISSGD
                     YPRSVIDFFINYEFLVPLMVRKS"
     gene            5942..6460
                     /locus_tag="IXT20_001833"
     CDS             5942..6460
                     /locus_tag="IXT20_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4860271.1"
                     /translation="MSTNKIAQLSLLSAACVAGRIAFQFIPNFQPMTAIFLFIVLYLS
                     LKDALIVMSLSIAISSFYFGVGPWVIGQWISYTVVLSLFSIVCLRKTVQRNLWLKGVC
                     FFLAGMVYGIGMTVFDTLLYRLPQPWIYYLQGVSFDLMHSIGNVVFFLVFLPVVKRFY
                     NHSGGKNEKNNL"
     gene            6441..6833
                     /locus_tag="IXT20_001834"
     CDS             6441..6833
                     /locus_tag="IXT20_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4430 domain-containing protein"
                     /protein_id="HAQ4860272.1"
                     /translation="MKKIIYSLLAMSVSILILTSCSTTYTQSASNQKEEVKTSQKATI
                     TLQENGQNFSEKEVFFKKGDDLLSILKRNFEVKEDNGFITSLEGHSQDEKNQLYWMFT
                     VDGKSATTGAKEIKLNDGQSVIFNLSKL"
     gene            6909..7844
                     /locus_tag="IXT20_001835"
     CDS             6909..7844
                     /locus_tag="IXT20_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HAQ4860273.1"
                     /translation="MENFKKSGMFSVIAALGANILVAISKFVGYAISGSAAMLNESIH
                     SIVDCSNQILLLFGDKQANKGQSELHQFGEGRAKYFFSTIVAMMLFFGGGALGVMEAF
                     EKLTHPSHEVGNPIIVIAILLFGLVIEGSSLRVAMKEIKELNTEKLSTMRFLRESRHS
                     EILIIFTEDLCAVIGLVLALAGTILTYATGIAAFDAISGLLIGFLLMGAAIFLAKEFY
                     SLIIGESVTASDLSKIKLAFERPEISRLIDIKTVHLSPTEILVAAKADIVGNKENEAY
                     SVINDIEKNMRQSLPDKKMYIYIETDKYDPNYSRK"
     gene            7977..8756
                     /locus_tag="IXT20_001836"
     CDS             7977..8756
                     /locus_tag="IXT20_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HAQ4860274.1"
                     /translation="MNKKKSAPWLQALHAVMPLCISYIPVGLACGVLLQKVGFDPLLS
                     GLLSILVFSGGAQFLVASMLTTQASFATTLLMVFFLELRYTLLGASLAGFMKKEKRTF
                     LAVFSQSLNDENYAVNYLKFSTDPSWNKRKALYVNWFSMTAWAGSNMIGNFFGSVIRV
                     DADLVHFALTAMFIFMFIMQMKSALLIFTGLFSGVLGVVFMVLFQNTFGLIAATVIAS
                     FAGFMLEKAFKKEKAKKLRDRGKDVNEPLFNFPEQEANHHD"
     gene            8749..9066
                     /locus_tag="IXT20_001837"
     CDS             8749..9066
                     /locus_tag="IXT20_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HAQ4860275.1"
                     /translation="MTKLYLLLLVACLFIVAYIPRLFPMLYFTHRKVPSWFSDWMKYV
                     PVALFAALAFKDVFITHEHLDIAWNIKIAAMILVAGVAYKTRSMALSVLTGLASVFLL
                     SML"
     gene            9310..10920
                     /locus_tag="IXT20_001838"
     CDS             9310..10920
                     /locus_tag="IXT20_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAQ4860276.1"
                     /translation="MNKKLMQGTFWLTFANLLCKVLGVVYLIPWLSMMGNNQDGMLAT
                     TLYNVGYLPYGLFLMLGTVGFPNAIAKKVAVATKEGDNAACRLIFRSTINIMFVIGIA
                     SAALMYLFAPLLAGISPIANVNNGILAIRSLCPSLIAIPILSAMRGYFQGKNDLRPYG
                     TSLIIEQAVRVIVILAGTYYLRVLTDGTILEAVLISTVASFFGGLAAIIHMYIVGRRK
                     DFFELKDFLISSRYFERENRSASVSIIRETLPFIFVGSVITIVQMIDQVTMKPLLHFF
                     RPEIADQQLEFLFSRASVNPNKLTLILISMVGTVAISSLPILSTMKKKDRIQIEKTVG
                     DSFSIALLILLPSLTGMSLLAGPLYTLFFGYDPESVGYFQMALLASLFFSLFTILSTM
                     LQSLNHHRFAIRLTVEAIILKVIFQVIGLGLVGGYGMSLSNGLAFGIVFVRGYHFMCK
                     EYRIAPLAKISNFFLKTFRSTLIMLAVCFAVFFLLNQRLSMESKSYAMIYCVAVGSIG
                     GLVFLFSQFGKNGFRMLKNVKHHSRSKE"
     gene            11077..11925
                     /locus_tag="IXT20_001839"
     CDS             11077..11925
                     /locus_tag="IXT20_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAQ4860277.1"
                     /translation="MKLAVVTDSSAYLPERIKNHPDLFVIPIPLIMDGKIYNEGIDIE
                     ADEYYGLLKNSKDFPTTSQPVVGEVLGLYEELKEKGYDTILSIHLSSGISGFVNTLFA
                     MKDDISGVTVIPYDSKITSMPMGHMVEAALDLNDEGQSLDEILAHIDRIRDNTYAYLI
                     VDDLNNLVRGGRLTNGAALIGSLLKIKPILTFSEGKIVLFEKIRSSKKAFARAEEIIG
                     RRNNEIQPPVKLYVIHANNLEVAIAEKEKLQKKYPNAEIEIGHFGPVIGTHLGEKAIG
                     IAISAQ"
     gene            12156..12635
                     /locus_tag="IXT20_001840"
     CDS             12156..12635
                     /locus_tag="IXT20_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4860278.1"
                     /translation="MEIPTNLKHKPVIVVEDYDKVDGRNALNTDAKGLSLGLAQWNDR
                     GKVDISGKVWRHTGEKWSRQSEELPITRILDLAILAAQGSLYFQDAYRYEKFYDPENP
                     VVDIIGLQGNRMTVEVDTKNPKIDEDIILYYDTLQKDGELIGERFRILKRLLDELGY"
     gene            12668..14896
                     /locus_tag="IXT20_001841"
     CDS             12668..14896
                     /locus_tag="IXT20_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HAQ4860279.1"
                     /translation="MENFSLTENRTFSRLEKQMIWKKPKSHRTSAEEWRIASEIKGNW
                     QDPEMKISNVLLEGDAGSGKTQLAKALSADLQLPYTKVTCFADMDKSDVFGALLPVVD
                     SNKEQDQELLEAIYQTDTLEAVLTLIQNHYGIDPFSAKEKLAELVQRIEADYPDAIHY
                     SYYPSEIVRAIEKGYLLEIQEPTVIRDASVLVALNSALEPNGMLNLPTGIIRRHPDCV
                     IVITTNRNYQGNRPLNESLRDRMQHAEKMDLPALEVMAERAMAKTMIQKQELLLKMAE
                     IIRLLDETAKANAIKGVAGMRSYFYWANTYKQGQDLLQSIYPKVLYKLSTDSDELAIL
                     EQALSDSGLLLELQDILRKEKWGHPQSEHTKGRTISAEEANERNITENAEEILRLDEK
                     KSLSAEEPNEGEEKQEETIEKNEIPPQMQKERSSDIEGKNQKEQQTGDDSQEMSASDM
                     EAHDKEIKKQLNKEARQIMKETIHEKEGLIVHRPAFQGKTPEARELHMQVMPIVESLS
                     RQILELLDNEQTETYQKGKYEGQRFNASKVAYGDLRNFDKKNPPHEQPSLAVAVRIDE
                     SGSMIRDDRIEAAKKAAIAIAEFSKKVDIPLLIYGDTADRSSREKTSLFSYKEFEDGF
                     QWLNEKLVTMKPRQNNRDGAALRLAAEKLNRQSATTKLLLNISDGQPKALPDYTGKKA
                     KEDIQSVLKEYERQGILFVSAAIGQDKEEIKSIYGESRFVDITDLNEFPKQMIQLIAR
                     YL"
     gene            15025..16668
                     /locus_tag="IXT20_001842"
     CDS             15025..16668
                     /locus_tag="IXT20_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HAQ4860280.1"
                     /translation="MIQEQKWWKEAVGYQIYPASFKDSNGDGQGDINGIREKLNYLKD
                     LGIGFIWINPIYQSPFVDNGYDVSDYQDIEERFGTMEDFDLLLKEAHELGIKVIMDLV
                     INHSSDQHQWFEESKKSKESPYRDYYIWVDGVDGKEPNNWTSIFGGSAWEYSHETGQY
                     YLHVFAKEQPDLNWESEKLKEELFNMIRWWLDKGIDGFRLDAISHVKKDEYSVKATEN
                     PFSPFQNVSGIEEHLTDLKHVFEEYDIMTVGEASGVTAEEGPQWVGKDGYFDMIFEFD
                     HIHIWQQEKEGQLDVLKLKLALSAWQTSLDGIGWNALYMENHDVPRAVSVFGDTRPDF
                     WAMSAKAIAMMYFFLQGTPFIYQGQEIGMTNMPFESIDQVDAVDSKRLYKRLLAEGKT
                     REEALDIIRETTRDNSRTPMQWTSEQYAGFSTHEPWLITNPNTKTINVEQQEYEPESV
                     LQFYKNMIRIRQTNKGLIYGSYKEYLHEHPQLYVYERYLEDEQYLIMVNLTESLADYE
                     LPKEADQSWTLLLSNSSSGEFEAKGILAPYEARLYKTNK"
     gene            complement(16741..17286)
                     /locus_tag="IXT20_001843"
     CDS             complement(16741..17286)
                     /locus_tag="IXT20_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01440 family protein"
                     /protein_id="HAQ4860281.1"
                     /translation="MKLSEKELKDQLTEIVNDILAEAHLKKGDLFVLGCSTSEVVGGH
                     IGKNSSAEVGQWIIRTLKELLDPKEIALAVQGCEHLNRALVVERTVAEAKNFEIVSVV
                     PALHAGGACSVAAFDQFNDPVEVEHVVGQAGIDIGDTSIGMHIKHVQVPVRPRLKTLG
                     QAHVTALRSRPKYIGGPRANY"
     gene            complement(17309..17794)
                     /locus_tag="IXT20_001844"
     CDS             complement(17309..17794)
                     /locus_tag="IXT20_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAQ4860282.1"
                     /translation="MKTKQLTIYAMLIALTVALSLTILIPVPATNGFVTLCEAGIYTA
                     AFLYGPTGGLVVGASSGLLIDLISGYPQWAVFSLVIHGLQGYVAGSFSKSSTRMWGLG
                     LLLGSLVMIIGYGIAGWILYDWPAGVASIPGNIFQNLIGMGLAFPITASLQRIQARKQ
                     Y"
     gene            complement(17784..18596)
                     /locus_tag="IXT20_001845"
     CDS             complement(17784..18596)
                     /locus_tag="IXT20_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylpyrimidine/phosphomethylpyrimidine
                     kinase"
                     /protein_id="HAQ4860283.1"
                     /translation="MKRILTIGGSDPFAGGGIQTDLKTFENFGLFGLSALTSIGALDI
                     NERFMLESISLELLKRQLVSIDKMTSLDGIKIGLLNSSEAIMVVRDFIKTKQNIPIIL
                     DPVLAFKETEAVINQLYISQLIEELFPLVTMITPNLAEAALLSGQALPSSLTEMIHLA
                     KELTASGTKNIVIKGGTRMAGDEAVDLLYSIDHYEVFSHKKIDTTTVNGAGCAFSSAI
                     TANLVLGADFTKAVAESKKFVAHCISNGVLMNDHTGSVWSGGIQKGGMRNEN"
     gene            complement(18735..>19843)
                     /locus_tag="IXT20_001846"
     CDS             complement(18735..>19843)
                     /locus_tag="IXT20_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HAQ4860284.1"
                     /translation="ILYLMEQPALSVSQSFLDKNEISYMTHTSGTTGIPKLICHSAHS
                     MGWRTKWQKTVFTKISEKKLIAFHISPVHSRFNIGISSLMSMGFPMMPLANAQSSKVV
                     HMLEAHRPIALETHPNNFVQWRFTAKEHPEAFSGIRYYHSTFDAINNQTMKTFLRTSM
                     AQDAVFLQVYGQSECGPMILKAHTLESLKTSDARDMGVGLEDMTSARITDSVGNVLPE
                     NTDGHIQLLSKGRALTYYKEDARFQENVYGDWWDSGDYGFMDKHGHLFLKDRQVDLIE
                     TINSTLAIEDFLLDSLDFLAEVVIVRDKNHSPQPIIALAPDKEMDWNRWWEQVHELPR
                     LNVPIIRDFDAIPRTATMKVQRLQIEKELKSQKS"
BASE COUNT         6488 a         3630 c         3906 g         5819 t
ORIGIN      
        1 tttctgcttt actggaacca tatttatgtt gagcatattg gtcccactct gcaaactcat
       61 ttcccttgat catctgattg aatgtattgt cagaaacaaa ataataatcc tcttgatctt
      121 tttccccagg tcttggtgat cgattcgtgt aagtaatgat cttttgttcc ggctgaaaaa
      181 tagcttctgc gagtgaagtt tttccagaac cagaaggacc gataaataca tagcaataat
      241 tcattgtcat ttctcctttg atgattgaac aagtgaaaaa gctttggaaa atatttccaa
      301 agcttttaaa agtaactttg cttttttgta tattatacca gcaagccagt cacaatagca
      361 cttaaaaagc tgactaaagt cgctgttagc aaaattttca atccgtgttt tgcgacaaga
      421 tttccttttt cttcatttaa tcccttcatc gcaccagaaa tgatcccgat agaagagaag
      481 ttggcaaatg aaacgaggaa gacagatata attgctgttg tacgctcagt aaaatggaaa
      541 ttaccatttg ctaattctgt catagcaaca aattcatttg taaccagttt agtcgccatg
      601 atactaccgg catccactgc ttctttccat gggattccgc tgatgaaggc aagaggggca
      661 aagatgtaac ctaggatctc ttggaaagtg atgccgaata tcccgtggaa gatagcattg
      721 atcatggcaa tcaaagcaac aaaaccgata agcatagcag caaccgtgat cgccacatgg
      781 aaaccatcaa gaatgtattc tcccaacatt tggaaaaatg tctgttcttt gttttcttca
      841 atgatcagct catcttcttt ttcttctaat acatagggat ttacaattga agccagaata
      901 taaccgccaa ataagttcaa aacaagggca gtgatcacat atttagaatc gatcagtgcc
      961 atataagatc cgacgatcga catagaaact gtagacatag ccgatacact catggtgaac
     1021 aaacgtttct cagataggaa gggtaattcc ttttttatgg aaataaaaac ttccgattgt
     1081 cctaaaatag cagaagctac tccattataa gattccaatt tgcccatccc gttgatttta
     1141 cttaatccta gaccaacagc tttcataaat aatggaagaa tcttgatata gcgtagaata
     1201 ccgataatag cagaaataaa tacaattggc atcaatacgg aaagaaagaa aggtgcactt
     1261 cctttatttg ctacaccggc aaaaacaaag tcaacacctt gcccagcaaa agcaagaaga
     1321 tgatcaaaca tttttgccaa tacagaaaca accgctgtac caaaagttgt acgtaataat
     1381 acgaaaccaa gaacaagctg taaaacgaac atcacaatta gtggtttcaa tttgattgct
     1441 tttttgttac tgctgatcaa aaaagcaagc cctaatgtga caaataaacc aataatacct
     1501 aataaataac tcaaataatc actcctattc caaattccgt tttattttac ctgttttgca
     1561 aataaaatac taatctttag tgaaattttc attacttttt tagagtgaaa cgcattcatt
     1621 ttccattaga aaataaagcg aacaaaataa aaggtttaaa aagtgtaacg tcttagtaaa
     1681 gtctataaaa aaacgaaact atcgattaag ctaatagttt cgttttcaac atttataagt
     1741 aaactttttc tattggtaat atagattttc ggaagggtag acaggtgcgc aggtaatatc
     1801 gattcctagt tttttcaatg tttgttcatc atttttattg accatcacag tagagtgggc
     1861 ttgtgctccc tcaagttcag ctagtttatc ataagcaact tgagcgattg gattcgttac
     1921 tgcactgatt gataaagcga tcaagacttc attgatgctt agtgcatcca aagaggaatg
     1981 aagtgtcttt gatttcaatc cttgtattgt ttcaagaact aatggtgaca ataaaggaat
     2041 gtcatctgcg atatgggcta aatgttttac tgcatttaag ataactgccg ctgacgcatc
     2101 catcaatgaa cttgtacgcc cggtaatgat gacttcatct ggtaattcga ttgcaatcac
     2161 agcagaagga tcttttgtag gactttgcgc ttgaatctta tctgcatagt catgggcagg
     2221 gactaccacg tttcggtctt caggacgtaa accgacttct tccataatca gtttgattcg
     2281 attaactgct tcttcgtcga tcaatccttt tttgaagtcg cattctgttt ggaaataacg
     2341 acggatgatt tcttgtttag aggcttcttg gacaacggta tcatccacga tcccaaatcc
     2401 tacacgattc acacccatgt cagtaggaga ttgataaaca gattctttac cagtaatttt
     2461 ttcaataata cgtttgatta caggaaatgt ctcaacatcc cgattatagt tcacagctac
     2521 tttattgtag gcatcaaagt ggaaggaatc gatcatattg acatctttta gatcgactgt
     2581 ggctgcttca taagcaatgt tcagcggatg tttcaacgga acattccata ctgggaaagt
     2641 ttcgaacttg gagtaaccag ctgcatgtcc tttttggctt tcatgataca gttggttcaa
     2701 ggaagtagcg agctttccac ttcctgggcc aggagctgtc actacaacga ttggtttagt
     2761 tgtttcaata tactcatttt ttccaaaacc atcttctgag acgatttttt ctacgttgac
     2821 tggatagtct tcgatttctg agtgtttata gaccttgatg tttcgtcttt ctagtttatt
     2881 gataaagact ttcgtggcag gctgtccact atagcgagtg ataacgacac tgttgattaa
     2941 taagccataa ccacgtaatt catcgattaa ccgcaagata tccatatcat aagtaatccc
     3001 ataatcacca cggatcttat tacgttcaat atctccagca tagacgcaaa tcagtatttc
     3061 agcttgatct ttcaattttt gcagcaattt gattttcgca tcttcttcaa atcctggaag
     3121 tacacgtttc gcatgcttgt cgtcaactag cttcccacca aattctaaat ataatttgtc
     3181 atagtggttc actcgttcca aaatatacgc tgactgctct tcaatatatt tttctgaatc
     3241 aaaaccaatt tttttcacta ggttccctcc ttaagtacta aagcattttt tcattttttt
     3301 gaggattcgt caactggaag agcgaaattc ctcaaaaaaa caaagaaaat ggtttaaacc
     3361 aacttgtcta gatagttatt cattggtatg atagtttttg ataaggaagg tgctacatga
     3421 aaaaaagaca gaaaaagaaa aacgcatata aacactatat ccgttcgatt tttaccggtt
     3481 atgaaagaat gcttgaagat cccgaattgg aacaattgac gtttacttat ctaaacgaag
     3541 aaacgcagct aactcgtgat gagcatcagc gcattcattt cacaacaaga gatctgccta
     3601 gtaaataaaa aataagataa aggtcatcgg attcgattct gtcctatcag atggaaagac
     3661 tagttgatcg aaaaaaaggc tgtaccaaat tttggtgcag cctgttttat ggagcattgc
     3721 ccgctctttt ttataacaat tttttacaga aaatggtact attgaagtat taataagtga
     3781 aaaacagatg agtaagagga ggcaagtagt atgaaagagc cagatacaaa tgcaaattat
     3841 gagccagacg aactagagga gacgaatgcg gatcaggata caggacaaga atcgtcttct
     3901 ttttacgata tcaaagaaaa agcaagcatg aatctttccc aattattaga agacttacag
     3961 gcttttgaaa aagctgtccg cgaagaagat atccctgaga tctaccgttt atacaatggc
     4021 cgattgaatg aagaattaaa aagaagctca aatgaaaatc acgagatcga tcaattgctg
     4081 atgcgtaaga tccatgatcg ttttttacag gaatttcctt ttatggaaca agtagaaaac
     4141 atctcgccaa cgatgagcta ttacaaaatc ggcacttatt accatgatcg gcccacggtc
     4201 ggtattgatg caagtctgcc agaaattttt gttttgccta agatcgatga agagtgggag
     4261 aaatattccc aaccggagtc aacggactat gacaaaaaaa tcaatgagct agacgcaaag
     4321 gtgatcactg ctcagacaga aattgaacgt ttagatgaac aaataaaaga aatcaatcga
     4381 caaatgactg atttgaacga caataaagga ttcttgaatc gtaaaaaaat cgaggaagaa
     4441 attcaagaat tagagaagaa gaagcaagta ctatctaatg aaaaattagg ctggcttcct
     4501 tatattgaga cgcctgaaac aatccagcag caaaaagaag cattgaagca agaagcaaga
     4561 gcagaccagc ttcgtgcagc catcgtagaa aaagaacaaa ggcaaatcag acgctatttc
     4621 ggtagcaaag agggattcgg tcaagcaatc catgaattct tgatgaatta tcttggcaat
     4681 gagaatccta ataatcaatc taatgaagga ggttccgagt atgaatgaac acgatgaagc
     4741 agaatggaca aaaaatgaag aacaaacaga agatcatgaa gaaacgaaca cttcattgga
     4801 agaacaaaca agtaatcctc tggatgacac agaagaaatc cctatgctag aggaaacaaa
     4861 aatacacgaa aaagaaaaag taactccgac gaatacgaat actgcagaaa acacccatgc
     4921 ttacgaaata actgaaaatc ccgggtattt agaaggaaaa gagcaagatc atcaagatat
     4981 cgtcagtttg attgaaacag cggaagcaga attagcggat ttacgtatga gaaaagattt
     5041 gatcgaatca gattttccac aattattaga atcatatgat cgttttatat tgaatgaaga
     5101 accaatctcc acgatcggaa agacaagcct gatcgaatac ttcacgtatg aattactgaa
     5161 accgttgaat gatattggtc tggaacggtc aaattcagct tatgacacat ggaaaacaag
     5221 acatttttcg atcaattata cgttgaatgg acaaaacgaa ttagtctttt cctttgtatt
     5281 accgaccatc gatcaacgat accaagtgga atcgatgagt ctcctaaacg tatcaccaga
     5341 gacaatggaa atcaatgtcc aagatgaccg ggtgctttct ttgatccgac actggtcagt
     5401 ggatcgagta ttctctgcag ggcagatcac gatattcaat cacaaattga atcaattatt
     5461 agcccatgca agaacacttg gttttaccgt taatcagaca ttactagaca atacgaagcc
     5521 acttcgtttg aatcttcaga gcgaatttga attgacgaat gaagtattgg atgatatttt
     5581 tatcgtggca atgaaaaatc catcctacga tatggaaaag atcaacgaaa ctacttatca
     5641 agtacgatta gataaaggac agtctttaac gataacaaca aacgaaaaag atcaaacagt
     5701 cttgtcgatc tcatctggag actacccacg atctgtcatt gatttcttta tcaattacga
     5761 attcttggtc ccattaatgg tgagaaagtc ctgattggat aagttggatt accagatgtc
     5821 attcagataa ttctgttcca aaggacttgc ggcagttatc cagaataatt gtcacaagtc
     5881 ttttctgtat gtactaaatg gacgtgaata acttgctgat gaaggaggga tttcattagg
     5941 aatgtccaca aataaaatag cacagcttag cttattgtct gctgcttgtg tagcaggtag
     6001 gatcgctttt caatttattc cgaatttcca gccaatgact gctatttttt tatttatagt
     6061 actttactta agcttaaaag atgcgttaat tgtcatgagt ctttctattg cgatcagcag
     6121 tttttatttt ggtgttggac catgggtcat cggccaatgg atcagctata ctgttgttct
     6181 gagtttgttt tcaatcgtct gcttgagaaa aacagtgcaa aggaatctat ggttaaaggg
     6241 agtctgtttt tttctggcgg gcatggttta cggaataggt atgaccgttt ttgatacgct
     6301 gttatatcgt ttgcctcaac cttggatcta ttaccttcaa ggggtgtcgt ttgatttgat
     6361 gcacagtata ggcaatgttg tgttctttct tgtttttctg ccagtcgtca aacgttttta
     6421 caatcattca ggaggaaaaa atgaaaaaaa taatttatag tttgcttgca atgagtgtca
     6481 gtatactcat tttgacaagt tgcagtacaa cctatacaca atcggcttcc aaccaaaagg
     6541 aggaagtaaa aacatcgcaa aaagcaacaa tcacgttaca agaaaatggt caaaactttt
     6601 cggaaaaaga agtgttcttc aaaaaaggcg atgatttact gagtattttg aaacgaaatt
     6661 ttgaagtcaa agaagataac ggcttcatca cttcgcttga agggcactca caggatgaga
     6721 aaaatcaact ctattggatg tttactgtgg acgggaaaag tgctactact ggtgccaaag
     6781 agatcaaatt gaatgatgga caatcggtca ttttcaattt gtctaaacta taagtttcat
     6841 aaaaaactgt taaagggcga atattgccat aattcagtga gttcagttga tgaaagaagg
     6901 aaaataacat ggaaaatttt aaaaaaagcg gaatgttttc cgtaattgct gctctagggg
     6961 ctaatattct agttgctatc agcaaatttg tcggctatgc aatcagtggc agtgccgcca
     7021 tgctaaacga aagcatccac agtatcgttg attgcagcaa tcagattttg ctgttattcg
     7081 gtgataaaca agccaataaa ggacagagcg agttgcatca atttggtgaa ggacgagcaa
     7141 aatacttttt cagtacaatc gtcgcaatga tgcttttctt cggcggtggg gcacttgggg
     7201 tgatggaagc ttttgaaaag ttgacgcatc catcccatga agtgggtaat ccgatcattg
     7261 ttatagcaat actgttattt ggtttggtaa tcgaaggaag ttcgttacgc gtggcaatga
     7321 aagaaatcaa agaattgaat actgaaaaac tttccacgat gcgctttttg cgcgaaagtc
     7381 gacacagtga aatactgatt atttttacgg aagacttgtg tgctgtaatc ggtttggtct
     7441 tggctttagc aggaacaata ttgacttatg caaccgggat tgctgcattt gatgcgatca
     7501 gcggattgct gatcggtttc ttgctgatgg gagccgccat tttcctagca aaagaatttt
     7561 atagtctgat catcggtgaa agcgtgacag caagcgattt atcaaaaatc aagttagctt
     7621 ttgaacgtcc tgagatcagt cggctgatcg atataaaaac agttcattta agtccaacgg
     7681 agattttagt tgcagcaaaa gcagatatcg ttggtaacaa agagaatgaa gcttactcgg
     7741 tcatcaatga tattgaaaaa aatatgcgtc agtctcttcc tgacaagaag atgtatatat
     7801 acattgaaac agataaatat gacccaaatt attctcgtaa ataaaacgtt tattcatgaa
     7861 ttgaagcaaa aatgagaaaa atataatctt gtttttaaat aaaacggcta caatttagcg
     7921 aatttatgtt acaatgagtc cgctgtttat ttgttgtaat taaaggagat attttaatga
     7981 acaaaaagaa aagtgcgcca tggcttcaag ctttgcatgc agtgatgcca ctttgtataa
     8041 gctatatacc agtaggatta gcttgcggag tattgttgca aaaagttgga tttgatcctc
     8101 tcttatcagg actgttatcg attctggtct tttccggtgg cgcgcagttt ttagttgcat
     8161 ctatgcttac gacgcaagca tcatttgcaa cgacattatt gatggttttc tttttagaat
     8221 tacgctatac attattagga gcaagtcttg ccggttttat gaaaaaagaa aagcggacgt
     8281 ttttggctgt cttttctcaa tcactgaatg atgaaaacta tgcggtgaac tacttaaagt
     8341 tttcaactga cccttcctgg aataaaagaa aggctttata tgtcaattgg ttctcaatga
     8401 ctgcatgggc cggtagcaat atgatcggta attttttcgg ctcggtgatt cgagtagatg
     8461 ctgaccttgt ccattttgct ttgacggcca tgttcatttt catgtttatc atgcagatga
     8521 aaagcgcact actgatcttt acaggtttat tctcaggcgt attaggggtc gtattcatgg
     8581 tattattcca aaatactttc ggattgattg cagcaacagt cattgcttca tttgccggat
     8641 ttatgctgga gaaggcattc aaaaaagaaa aagctaagaa actaagagac cgcggtaaag
     8701 atgtcaatga gccgctcttc aacttcccgg aacaggaggc caatcaccat gactaaacta
     8761 tatttgctac ttttagttgc ttgtctgttc atcgtggcat atatcccgcg cttgttcccg
     8821 atgctttatt ttactcatcg aaaagtgcca tcctggttca gtgactggat gaaatatgta
     8881 ccggtggcat tatttgcggc gttagctttt aaagatgttt tcattactca tgaacatctg
     8941 gatattgctt ggaatatcaa aatagctgca atgattctag tagcaggcgt ggcttacaaa
     9001 acacgttcga tggcgttgtc agttttgaca ggacttgcgt ctgtcttttt gctgtctatg
     9061 ctttaaactg ttttatttta ttaaaaacag agattgcgac ataagttgtt caaactgatc
     9121 tatccgaact gcatttgcaa cagtcaattc tggatgcaag tttattcgta gggataaaaa
     9181 agattagtgt tggcttatga tgcaagctct tttttatttt aggaattaga gagggatttg
     9241 tccttctatg attttttgct ataataggga agatgtttat tttatgaaag aatagttgag
     9301 ggaatttaaa tgaataaaaa attgatgcaa ggaacatttt ggctaacctt tgctaatctt
     9361 ctatgtaaag ttttaggggt agtctatttg attccctggc taagtatgat ggggaacaat
     9421 caagatggta tgcttgctac gacattgtat aatgttggct atcttcctta tggattgttc
     9481 ctgatgcttg gtacagtcgg atttccaaat gcgattgcaa aaaaagtagc tgtagccaca
     9541 aaagaaggag ataacgctgc ttgtcggctg atttttagaa gtacgatcaa tatcatgttt
     9601 gtcatcggta tcgcctctgc tgcactgatg tatttatttg ctcctttact agccggaatc
     9661 agtccgattg ctaacgttaa taatgggata ctagctatca gaagtttatg cccatctttg
     9721 attgctatcc caattcttag tgcaatgaga ggatatttcc aaggaaaaaa tgatctgcgg
     9781 ccttatggaa cttctttgat tatcgaacag gctgtgcgag tgatcgttat cttggctggt
     9841 acttattact tgcgcgtatt gactgacgga accatattag aagctgtatt gatcagtaca
     9901 gtcgcttctt tcttcggtgg gttagctgcg attatccata tgtatatcgt cggacgcaga
     9961 aaagacttct tcgaacttaa agattttttg atttccagcc gttatttcga aagagaaaac
    10021 cgatctgcct ctgtctctat cattcgggaa acactaccgt ttatttttgt cggctcagtc
    10081 attactatcg ttcaaatgat cgaccaagta acgatgaaac cactgcttca ctttttccgt
    10141 ccagagatcg cagatcagca actggaattc ttattcagcc gtgcttctgt caatccaaat
    10201 aaattgacat tgatcctgat ctccatggta ggaacagtag ctataagcag cttgccaata
    10261 ttaagcacga tgaagaaaaa agatcggata caaatcgaaa aaacagtagg agatagtttt
    10321 tcgattgcgt tattgattct tcttccttca ttgactggaa tgtctttatt agctggacca
    10381 ctatacacat tgttttttgg ctatgatcct gaaagtgtcg gatatttcca aatggctttg
    10441 ctagcttctt tgtttttctc tttattcacg atcctatcta cgatgcttca atctttgaat
    10501 caccatcgtt ttgcgatccg attaactgta gaagcaatta tcctgaaagt catcttccaa
    10561 gtaatcggat taggattagt tggcggatac gggatgagct tatcaaatgg cttagcattc
    10621 ggtattgtct ttgttcgcgg ctatcacttt atgtgcaaag aataccgaat agcaccttta
    10681 gcaaagatca gcaatttttt cttaaaaact ttccgctcca ccctgattat gcttgcagta
    10741 tgttttgccg ttttcttttt gctgaatcag cgattgtcaa tggaatcaaa aagctatgca
    10801 atgatttatt gtgtggctgt aggaagtatt ggcggcttag tatttttatt ttctcaattc
    10861 ggaaaaaatg ggtttcgaat gttgaaaaat gttaaacatc atagcagaag caaagaatag
    10921 gttaactaat cgttttttgc tgtcacttga aaaacggcaa ctcgtatgat tacgcttgtc
    10981 gtttttctat tttccaattt tagtttcata tctaatctta cttatgatat agttataagg
    11041 tctggaaaaa ttctttgaaa cgaggaaatt aaacacatga aacttgcagt agttacagat
    11101 agttcggcat atctgccgga acgtatcaag aaccatcccg acttattcgt tattccaatc
    11161 cctttgatta tggatgggaa aatttataac gaaggaatcg atatcgaagc ggatgaatat
    11221 tatggacttt taaaaaacag taaagatttt cctactactt cacaacctgt cgtaggggaa
    11281 gttttgggtt tatatgagga attgaaagag aaagggtatg acacgatttt aagtatccat
    11341 ctttcttcag ggatttctgg ctttgtcaac acattatttg cgatgaaaga tgacatcagc
    11401 ggagtaacgg tcattcctta tgactctaag atcactagta tgccgatggg acacatggta
    11461 gaagctgcct tagacttaaa tgatgaaggt cagtcccttg atgaaatact tgcacatatc
    11521 gatcgaatcc gagataatac ctacgcttat ctgattgtag atgatttgaa caatttagta
    11581 cgaggaggac gtttgacaaa tggtgcagcg ctgattggca gcttattaaa aatcaaaccg
    11641 atcctgacat tttctgaagg gaaaattgtt ttattcgaaa aaatccgttc aagtaaaaaa
    11701 gcatttgctc gagctgaaga aattattggt agaagaaata acgaaataca accgccagtc
    11761 aaactttacg tgatacatgc aaataattta gaagtagcga ttgcggagaa agagaaattg
    11821 cagaaaaaat atccaaatgc agaaatcgaa attggtcatt tcggaccagt cattggtaca
    11881 catttaggtg aaaaagccat cggcattgcc atttcagcgc aataattcct tttattcaaa
    11941 aaacatacca aaaatattcc aacatcatct tggactttct acatgttact aatgagcgtg
    12001 taaaagaacg agatggtgtt tttatttcta tcaatgaagc tctttttctt tgcaatcctc
    12061 acttcaatcc tctataatgt cgcatatagt ttagtaaatg agtaaactac taatttactt
    12121 atttactaaa aataaaagga tgaggagtta aaataatgga aataccaaca aatttaaaac
    12181 ataaaccagt aatcgttgta gaagactatg ataaagttga tggaagaaat gcattaaata
    12241 cggatgcaaa agggctttca ctgggccttg ctcagtggaa tgatcgtgga aaagtcgata
    12301 tctcaggcaa ggtatggcga catactggag aaaaatggtc acgacagtcg gaagaattac
    12361 caatcacacg tattttagat ttagcgatct tagctgcaca aggaagttta tacttccaag
    12421 atgcttatcg ttatgaaaaa ttttatgatc cagaaaatcc agtcgtagac atcattggat
    12481 tacaagggaa ccgaatgacg gtagaagtag ataccaaaaa tccaaagatc gatgaagaca
    12541 tcattttgta ttacgataca ctacaaaaag acggagaact gattggggaa cgattccgta
    12601 ttttaaaacg tcttttagat gagctaggat attaatagac agagatgatc aaaaggggaa
    12661 gagaagcatg gagaatttct cactaacaga aaaccgaacc ttttcacggc tagaaaagca
    12721 aatgatctgg aagaaaccga aaagccatcg gacaagtgca gaagaatggc ggatcgcctc
    12781 cgaaatcaaa gggaattggc aagatcctga aatgaaaatc agtaacgtac ttttggaagg
    12841 agatgccggg tcggggaaga cacaattagc caaagcacta tctgctgatc tgcaacttcc
    12901 ctatacaaaa gtgacctgtt ttgcagatat ggacaaatca gatgttttcg gagccttatt
    12961 acctgtagta gattctaata aggaacaaga ccaagaatta ctagaagcta tttaccaaac
    13021 agatacactt gaagctgtac tgacactgat ccagaaccat tacggcattg atcctttttc
    13081 tgctaaagaa aaattagcag agctagttca aaggatcgaa gctgattatc cagatgcaat
    13141 ccattacagc tattaccctt ctgaaatcgt tcgagcgatt gaaaaaggat atttattaga
    13201 gattcaagag ccgactgtca tccgagatgc ctctgttctt gtggcactta actcagcgct
    13261 cgaaccaaat gggatgttga atttaccgac aggaatcatt cgtcgccatc cagattgcgt
    13321 cattgtgatc actacaaatc gaaattacca aggaaatcgc ccattaaatg aatctttaag
    13381 agatcggatg caacatgcgg aaaaaatgga tcttcctgcc ttagaagtca tggcagaacg
    13441 tgcgatggcg aaaactatga tccaaaaaca agaacttctc ttaaagatgg cagagattat
    13501 ccgtttgttg gatgaaactg caaaagctaa cgccatcaaa ggtgtagcag gaatgcgttc
    13561 ttatttttat tgggcaaata cctataagca aggacaagat ttactccagt cgatttatcc
    13621 aaaagttttg tataaattat ccactgattc agatgaattg gcaattcttg aacaagcttt
    13681 gtctgacagt ggattgctac tggaacttca ggacatattg agaaaagaaa aatggggtca
    13741 tcctcaatct gagcatacaa aaggaagaac cattagcgcc gaagaagcaa atgaacgaaa
    13801 catcactgaa aatgctgaag aaattctgcg cttagatgaa aagaaatcgt tgtctgctga
    13861 agaaccaaat gagggagaag agaaacaaga agaaacaata gaaaaaaacg agattcctcc
    13921 tcaaatgcaa aaagaacgca gtagcgatat cgaaggaaaa aatcaaaaag aacaacaaac
    13981 aggagatgat tctcaagaaa tgtctgcttc tgacatggaa gcacatgata aggaaatcaa
    14041 gaagcaactg aataaagaag cgcgacaaat catgaaggaa acgattcatg aaaaagaagg
    14101 tctcatcgtt caccgaccag ctttccaagg aaaaacgccg gaagcacgtg agctacacat
    14161 gcaagtaatg ccaatagtag aatcacttag ccggcaaatt ttagagttac tggataatga
    14221 acaaacagag acgtaccaaa aaggaaaata cgaaggtcag cggttcaatg caagcaaagt
    14281 agcatatggt gatttgagaa attttgacaa aaagaacccg ccacatgagc aaccttcttt
    14341 agcggtagct gtacggatcg atgaatctgg gtcaatgata cgggatgatc ggatcgaagc
    14401 ggcaaaaaaa gcagcgattg ccattgccga attttctaag aaagtcgata tcccattgtt
    14461 gatttacgga gatacagcag accgttcgtc aagagaaaag acttctttgt tttcttataa
    14521 agaatttgaa gacggatttc aatggttaaa tgaaaaatta gtcactatga aaccaaggca
    14581 aaacaatcgt gatggtgctg cacttcgttt agcagcagaa aaattaaaca gacaatccgc
    14641 aacaaccaaa ctgttgctta atattagtga cggtcagcca aaagcgttgc cggattatac
    14701 aggcaaaaaa gcaaaagaag atatccagtc tgttttgaaa gaatatgaac gtcaaggaat
    14761 cctatttgtc tccgcagcta ttggccaaga caaagaagaa atcaagtcga tctacggtga
    14821 aagtcgtttt gtagatatta ctgacttgaa tgaatttccc aagcaaatga ttcaactgat
    14881 tgcccgctat ctatgataaa ataaagctca acgaacgcac ttagcagatt cgttgagctt
    14941 ttcgtttcta gttaccggta acaaggatac ttctaaaata gaatcggtta taatcaagag
    15001 ggaccgttaa aggagtgaaa cgatatgata caagaacaaa aatggtggaa agaagcagta
    15061 ggctatcaaa tctatcctgc tagtttcaaa gatagcaatg gcgatggtca aggagacatc
    15121 aatgggatcc gtgaaaaact taattatttg aaagatcttg gtatcggctt tatctggatc
    15181 aatccgatct atcaatctcc atttgtcgat aatggttatg acgtaagtga ctatcaagat
    15241 atagaagaga gattcggaac gatggaagat tttgatttat tgctaaaaga agcacatgaa
    15301 ttagggatca aagtgattat ggatcttgtc atcaaccata gttcggatca gcatcaatgg
    15361 tttgaagaat cgaaaaaatc taaagaaagc ccgtatcgcg attattacat ttgggtcgac
    15421 ggggtagatg gcaaagaacc gaataattgg acatcgattt tcggcggttc tgcttgggag
    15481 tattctcatg aaactggaca gtattatctg catgtttttg caaaagaaca accagactta
    15541 aattgggaaa gcgagaaatt gaaagaagaa ctattcaata tgatccgatg gtggttggat
    15601 aaagggatag atggatttcg tttagatgcg atttctcatg taaaaaaaga tgaatattca
    15661 gtaaaagcca cagagaaccc tttctcaccc tttcaaaatg tctcaggaat cgaagaacat
    15721 ttgaccgatt tgaaacatgt ttttgaagag tacgatatca tgactgtcgg agaagcaagt
    15781 ggtgtcactg cagaagaagg accacaatgg gtagggaaag acggttattt cgatatgata
    15841 ttcgaatttg atcatatcca tatctggcag caagaaaaag aagggcaatt agatgtcctt
    15901 aaattgaaac ttgcattaag cgcttggcaa acttcattgg atggtatcgg atggaacgct
    15961 ttatatatgg aaaaccatga tgttcctcgt gccgtttcag tgtttggaga tactcgtccc
    16021 gatttttggg ctatgtctgc aaaagcaatc gctatgatgt atttcttttt gcaaggtacg
    16081 ccatttattt atcaaggaca ggaaatcgga atgacgaata tgccctttga atcgatcgat
    16141 caagttgatg cagtagattc gaaaagactt tataaaaggc tgcttgcgga aggtaagaca
    16201 agagaagaag cattggatat cattcgtgaa acaacgagag acaacagcag gacacccatg
    16261 caatggactt cggaacaata tgctggtttt tctacacatg aaccttggct gatcactaat
    16321 ccaaatacca aaacgatcaa tgtagaacaa caagaatatg aaccagagtc ggtcctgcaa
    16381 ttctataaga acatgatccg cattagacaa accaataaag gtctgatata cggttcttat
    16441 aaagaatatc ttcatgaaca tccacaactt tatgtctatg aaagatattt agaagatgaa
    16501 caatatctta ttatggtaaa cttaacagaa tcacttgctg actatgaact tccaaaagaa
    16561 gcagatcagt cctggacatt actcttgtca aattcctcta gcggagaatt tgaagctaaa
    16621 ggcatactag caccttatga agctcggtta tataaaacaa acaagtaaaa cttcctcgtt
    16681 aaagttcatt agttggactt tgcgaggaag tttttcagat tgctctgtca attactcatt
    16741 tcaataattc gctctaggac caccgatata tttaggacga gagcgaaggg ctgtcacgtg
    16801 agcttgtcca agggttttca aacgaggtct gacaggtact tggacatgtt tgatatgcat
    16861 accgattgat gtgtcaccga tatcgatacc ggcttgtcca actacgtgtt ccacttcgac
    16921 aggatcattg aactggtcaa aagcagcaac agaacaagca cctccagcat gtagtgcagg
    16981 aacaacagag acaatttcga aattttttgc ttctgccact gttcgttcta ctactaatgc
    17041 tcggttcaaa tgttcacaac cttggacagc tagcgcaatt tctttaggat ccaacaactc
    17101 ttttaacgta cggatgatcc attgaccaac ttccgcactg gaattttttc caatatgtcc
    17161 tccaacgact tcgcttgtgc tgcatcctaa aacaaaaaga tcaccttttt tcaagtgtgc
    17221 ctcagccaat atgtcattaa ctatttcagt caattggtct tttaattctt tttctgacaa
    17281 cttcattttc taaaaccttc tttttcaatt aatattgctt ccgtgcttga atacgttgaa
    17341 gacttgcagt tataggaaag gcaagtccca ttccaatcag attttggaag atgtttcctg
    17401 gtatagaagc cactccagcc ggccaatcat ataaaatcca accggcgata ccatagccga
    17461 tgatcataac taagcttcct aataaaagcc ctaatcccca catacgagta gaagatttgc
    17521 tgaaagaacc agcaacatat ccttgtaacc cgtggatcac taatgaaaaa actgcccact
    17581 gcggatatcc tgaaatcaaa tcgatcaata gaccactgga agcaccaaca actaatccac
    17641 ccgttggacc ataaagaaaa gcagcggtat agatcccggc ctcgcataat gtcacaaatc
    17701 cattcgtagc cggtacaggg atcaaaattg ttaatgaaag ggcaactgtc aacgcaatca
    17761 gcatagcata gatcgttaat tgcttagttt tcatttctca ttcctccttt ttgaataccg
    17821 ccagaccaga cacttccggt atgatcgttc atcagcacgc cattagaaat acaatgggca
    17881 acaaattttt tactttctgc tactgctttg gtaaaatctg cccctaacac aagattcgct
    17941 gttattgcag aagaaaaggc acaaccggcc ccgtttactg ttgtagtgtc gattttttta
    18001 tgagaaaata cctcataatg atcaattgaa tagagaagat ccacagcttc atctccagcc
    18061 atccttgtgc cgcctttgat cacaatgttt tttgttcctg aagcagtgag ttctttagcg
    18121 agatggatca tttctgttaa cgaagaggga agagcttgac cagaaagtaa agcagcttct
    18181 gccaaattag gcgtaatcat cgtaacaagt ggaaaaagtt cttcgatgag ctgcgaaata
    18241 tacaattgat tgatcacagc ttcagtttct ttgaatgcca aaacagggtc taaaatgatt
    18301 gggatatttt gttttgtttt tatgaagtct cgaacaacca tgattgcttc acttgaattc
    18361 aataacccaa ttttgatacc atctaatgaa gtcattttat caatcgaaac taactgtcgt
    18421 ttaagcaatt ccaaagatat agattccagc ataaatcgct cattaatatc tagtgcccct
    18481 atagaagtca aggcgcttag tccgaataga ccaaaattct caaaagtttt taaatctgtt
    18541 tggattcctc caccagcaaa tggatcagaa ccgccaattg tcaagattct tttcataaca
    18601 ttttcctcca tgaataagag tataacgaaa ttttcaaaaa agaaaaacag ccaattggca
    18661 tatttttatc tcatccaatt taaaaaaaca aaaagaaaag aaagaccaaa atggatcttt
    18721 ctttccttaa atagttagga cttttgtgat tttaattctt tctcgatttg aagccgttgc
    18781 actttcattg tagctgttcg cgggattgca tcaaaatcac ggatgatagg gacgttcaag
    18841 cgaggaagtt catggacttg ctcccaccat cgattccaat ccatttcttt atcaggagct
    18901 aaagcaataa tcggttgtgg agaatgattt ttatccctta caatcactac ttctgccaaa
    18961 aaatctaacg aatctaataa aaagtcttca atagctaatg tactattgat cgtttcgatc
    19021 aaatccactt gccgatcttt caaaaatagg tggccatgct tatccataaa accgtaatct
    19081 ccgctatccc accaatcgcc ataaacattt tcctggaacc gtgcatcttc cttgtaataa
    19141 gtcaaagccc gtcctttaga cagtaattgt atatgaccat ctgtgttttc aggtaggaca
    19201 ttcccaactg aatctgtaat ccgagcgctt gtcatgtctt ccagtccaac gcccatatct
    19261 cttgcatcac ttgttttcaa actctctagt gtatgagcct taagaatcat tggtccgcat
    19321 tcactttggc catatacttg aagaaatacc gcatcctgtg ccatcgacgt tcgcaaaaat
    19381 gtcttcattg tctggttgtt tatagcatca aaagtggagt gatagtatcg gatacccgaa
    19441 aaagcttctg gatgctcctt agcagtaaaa cgccactgaa caaagttatt cggatgggtc
    19501 tctagtgcaa ttggccggtg agcttccaac atatgaacaa cttttgaact ttgtgcgttt
    19561 gctaaaggca tcataggaaa tcccatggac atcaaagaag agataccaat attgaatctg
    19621 gaatgcactg gagagatgtg aaaagcgatt aatttctttt ccgaaatttt tgtgaaaact
    19681 gttttttgcc atttcgtccg ccatcccata gaatgagcag aatgacaaat aagtttaggg
    19741 ataccagttg taccagaagt atgggtcata taagaaattt catttttgtc taaaaatgat
    19801 tgagaaacag acaaagcagg ttgttccatt aaataaagaa tag
//
LOCUS       DACGLE010000048        19452 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0534
            SAMEA3304449-rid9560453.denovo.052, whole genome shotgun sequence.
ACCESSION   DACGLE010000048 DACGLE010000000
VERSION     DACGLE010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304449
            Sequence Read Archive: ERR985064
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19452)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19452)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702225.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702225.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 20:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,045
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 27 of 67
            Pseudo Genes (incomplete)         :: 25 of 67
            Pseudo Genes (internal stop)      :: 19 of 67
            Pseudo Genes (multiple problems)  :: 4 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19452
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304449-rid9560453.denovo.052"
                     /strain="VREN0534"
                     /db_xref="taxon:1352"
     gene            133..3660
                     /locus_tag="IXT20_001847"
     CDS             133..3660
                     /locus_tag="IXT20_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HAQ4860285.1"
                     /translation="MSLQFILGNGKKDHRQVLIDEASAWLDQDASNQVFFLVPNYSKF
                     EQEQEILAEMRRRSGKKTFSTTNVQVFSFYRLAWYLLQQTTLLTGNELSESGSAMILR
                     QILEKNEEELTIFRGEINKRGFIRQLQELYSELRTGNIQPEDLMLLFGDSPNAEDQQL
                     KMKDLKLIFSAYDSELTQRALTNEDALSILANYMQTQDFNNVKFIVSGFSRINGQEYQ
                     LLQAMMEKGQLVVDLLLDRPYTADLPEVLTLFHETGKLYFRLFQSARERKVPILVDRF
                     APEQTRPTELQLLQTMWEETSSQKKAAVHKIGGQEHLHLWEAENPTEEVRQLAVEIRR
                     LVSEENYRYRDIQVLTKNMSLYGNILRPIFQEMAIPFYIDEEQMMENHPLIEWINSLF
                     ALDRYSYRLSDIMRFFKTELFLSTEAETELDEWKQERDEFRKKLDITENVALAYNYQG
                     TYWTREKDWQFVAYDFEAEQIEDVQYLEEQSNTIRRLFQKAVPVFLKKIKKSKTGQEA
                     ANVFYQFLIQSGVQQQLLFWRNQEVDRGNLEAARNHEQTWGALIDLLDEYVVIYGESA
                     FDWSLFQEIITSGLENLSYGKIPTAIDQVRINRLELVRPNQAAVTFALGLNDQVFPDR
                     KETKGLLSSEEREVLNQALPDGQFLFDPSKESISFEPFQAYLVFLSGTDRLYLSYAQS
                     YDTDGSLKMSPYLRRITEYLSVPVERKEHLTIESDPNCYVGTYRSGINTVNRIYRLAL
                     DEKKPVPKHWQKLKELVLNSSWRNFALQIFESQEHLNVPVSMPEKMAEELYGKEIYTS
                     ISRMENFYNCEYKYFMQFGLKLKERTVYGLTPAATGDFYHESLDRFFKLLFSNQLSLV
                     NMSDAQRRDFTEKVLQEVFGELRFEILDSSARMNYIRYQLGQTIQKVTWALQKQSKNT
                     GMHPLQTEILFGQIAGAKGITGLELPLSNGGKLHVRGKIDRIDVASEQEDTWLSVVDY
                     KSSGRSFDVTEAYYGMAMQLLTYLDVALMDAVRLTGKAAVKPAGSYYLHVHNPVLTET
                     DDVEKNTLKKFSYDGVFVDDPALLEVLDHSLEAKEQSLIYPVKKNAKDEYQKGSFSKD
                     KFFTEEELQLFMAHNRENMRLAGDAITSGEIKLNPAYKDKQRIACEFCPFRSICTFDV
                     MLKENNYHRLEKIDKEEALRRILKRSEEE"
     gene            3657..7379
                     /gene="addA"
                     /locus_tag="IXT20_001848"
     CDS             3657..7379
                     /gene="addA"
                     /locus_tag="IXT20_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HAQ4860286.1"
                     /translation="MNQIPLKPQNERFTDDQWQAIFDQGDNLLVSASAGSGKTTVLVR
                     RVIEKLKMGFDIDELLIVTFTEAAAREMKERIQEALQESVNSESDPVRRQHFTKQLVL
                     LPTANISTLHAFCLTVIRRYYYLIDIDPVFRMLTDETETILMKEDVWDELREALYAEN
                     DERFFQLTMNFSNDRSDDGLTNLVFSLYEFARANPDPQKWLEQLSDNYRLPEGLAKSR
                     LYQKQIRPLVLADIYQCVQLYEQMTQLAQGEGLEKMHEQVAGEQQQIKNIYEAFSQDR
                     LEEAYAGLEQLTFSTFKSSRKAELKEISNEVKGMRDKAKKLIQQISKSYFPVSPSQME
                     ELTDKALPLVEEMTKVTQSFMDGFSMRKREKGVLDFNDLEHLALQILTEKTKDVWLPS
                     EASKHYRKKFKEVMVDEYQDVNQLQEAILYWLREPDDTKGNMFMVGDVKQSIYSFRLA
                     DPSLFIGKYENFSKKEGGRRIVLAENFRSRKEVLSFTNLIFEQLMDPAVGQINYDEAA
                     KLIQGFSDFPENEQFEPEIMIYEKEQEESEIEIPTDDILEDKTEGELFMTGLKIRQLI
                     DSSFMIYDKKSKKSRPIEYKDIVLLTPTKKNNLTILEIFKTLDIPLEMNDAQNYFQAT
                     EIRTMISLLQLIDNPYQDIPLAAVLRSPIVGLIEPELASIRLANRAHTYYDAVLAYQA
                     SNEDELAAKLEHFGKQLEHWRELARRSSITDLLWDIYYETGYLEYVVGLPAGAQRQAN
                     LYALVDRAKAYEQSSFRGLYQFVRFIEKMQEKDKDLAEPVISIEDNAVRVMTIHASKG
                     LEFPVVFLLDMTKEFNLQDLRNRYAFEEKLGAGIRYMDPETRVLYDTLPFQAIKLAKQ
                     NKLLSEEMRKLYVGLTRAEQKLFIVGSYKNKEQMIQTWSEAADHEELVFDPALRLKGR
                     SSLMNWIGYGLIRHPEMQKYLEEEISTSLLQHSNAQFSISWMNQQSIIEQRQLLAEKE
                     LVNLDQQMKEDETLLADSLQKRLAYEYPYQASSQTTSYQSVSEIKRLFEDPDDTQESR
                     LTLESSQNKAASRQFRYTQEQLAEPKFLQKDRQVSAAAVGTATHALLQLLPLEMPTTE
                     SIHQKLQELVRKRLVDEKVAKKVDVSSIIWFFQTELGQQLIANKENVKREQPFSMLLP
                     ADEVFQDYPNQEDELLIHGIVDGYLEEKDHLNIYDFKTDFILHPDDPAEIDAIVQKYQ
                     GQLRLYQQAMSEALNKPVENVFLILLRVKQIININK"
     gene            7448..7789
                     /locus_tag="IXT20_001849"
     CDS             7448..7789
                     /locus_tag="IXT20_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ4860287.1"
                     /translation="MELTQTEFVLCPKFEKTFSILGKKWNGLIIDVLLENGPQRFVEL
                     AAKIPMVSDRVLVERLKELEKEGIVTRNFACSEKSRSEYLLTEKGADLQKAMRQIQVW
                     SEKWITEEECG"
     gene            8098..9141
                     /gene="pheS"
                     /locus_tag="IXT20_001850"
     CDS             8098..9141
                     /gene="pheS"
                     /locus_tag="IXT20_001850"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HAQ4860288.1"
                     /translation="MSLQAQLESLKEATLVKITAAQDLNELNQIRVETLGKKGPITEV
                     LRGMKDLSPEERPKVGSFANEIRDLLTEEIVRKKQVLEEEALNAALAKETIDVTLPGR
                     KVKHGTRHIVTQIMEEIEDIFVGMGYQIVEGYEVESDHYNFERMNLPKDHPARDMQDT
                     FYISDEILIRTHTSPVQARTMEKHDFSKGALRMISPGKVFRRDTDDATHSHQFHQIEG
                     LVVDKNITMGDLKGTLEVVMKKMFGEDREIRLRPSYFPFTEPSVEVDVSCFKCGGAGC
                     NVCKYTGWIEILGAGMVHPNVLKMSGIDPEEYSGFAFGLGPDRVAMLRYGVNDIRNFY
                     QNDLRFLSQFKGE"
     gene            9146..11566
                     /locus_tag="IXT20_001851"
     CDS             9146..11566
                     /locus_tag="IXT20_001851"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HAQ4860289.1"
                     /translation="MLVSYKWLNRYVDLSNVTPKELADKMSVTGIEVEGVTVPEEGLK
                     KIVVGEVKECVPHPDSDHLSICQVDIGEDELSQIVCGAPNVKVGIKVIVALPNSRIAG
                     NVKIKKGKMRGQVSNGMICSLQEIGYSDSVIPKEYAEGIYYMPQEAVNGDPVFPYLDM
                     DDSIIELSITPNRADALSMRGVAYEVGAIYRQTPTFDDEKLVESSRPASDKIQVEVED
                     KEAVPAYQIRIIEGVRIQPSPQWLQNLLMNEGIRPINNVVDVTNYILLLFGQPLHAFD
                     YDKLKSKEIFVRRAKENETLVTLDGESRELTTDNLVITNGKEPVALAGVMGGLDSEIT
                     NETTTVALESALFDPISIRRTAKQFNLRSESSARFEKGINHATVGEASEVAAAMIVSL
                     AGGEVLQGAVKGSEIKAEDVSVKISLERINQYLGTSLVVKEVNEIFEALGFSYEEKEY
                     VYSVVVPPRRWDIQIEADIIEEVARIYGYDQLPSTLPSGETVAGSLSKEQETTRKIRT
                     IFEGSGLSEAISYALTTEEKSRQFVINESNITRLDWPMSEERSVLRMNLISGLLDDIS
                     YNTARKNTEIALYEIGRVFFQENDPRTHLPQEVKHAAIAISGIWEEKDWQTKAQNVDF
                     FTIKGLLENLFEQLGITEDVHYQTVTEMKEMHPGRTAAIYLGEKFIGFVGQVHPTITK
                     SYDIPETYVAEFDLSAIVEAAQKGIVFEAVTKFPAVSRDIALLVKETVTNQELTEVIR
                     SAAGRFLTDIQLFDVYQGENIEKGHKSMAYSLTFVNPEATLTDEEINKAMEKVEKALV
                     ENLEASIR"
     gene            complement(11736..12746)
                     /gene="trpS"
                     /locus_tag="IXT20_001852"
     CDS             complement(11736..12746)
                     /gene="trpS"
                     /locus_tag="IXT20_001852"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HAQ4860290.1"
                     /translation="MKNIILTGDRPTGKLHLGHYVGSLKTRVAMQADEDNQLFVMIAD
                     MQALTDNAKNPEKVSSNVLQVALDYLAVGLDPAKSTLFIQSQIPELAELTMYYLNLVS
                     VGRVRRNPTVKTEIEQKKFGESVPTGFFIYPVSQAADITAFKANLVPVGEDQKPMLEQ
                     TQEIVQSFNHTYGEVLVEPKGVFPPKGMGRLPGIDGNGKMSKSLGNGIYISDPADVLQ
                     KKVMSMYTDPNHIHVQDPGQVEGNMVFTYLDVFGTDKEAIEEMKAHYRRGGLGDVKIK
                     RYLIDVLEAEFAPIRARREELEKDPSAVMEILRKGSEEAAKVAAQTLSEVKSAMGINY
                     FH"
     gene            13000..13737
                     /locus_tag="IXT20_001853"
     CDS             13000..13737
                     /locus_tag="IXT20_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HAQ4860291.1"
                     /translation="MAMIKFDHVDKYYGKFHALKNINLEFEKGEVVVVIGPSGSGKST
                     MLRCINGLETISSGKLLINDTDLHDKKTKLTEVRKNIGMVFQHFNLYPNKTVLENIKL
                     APIKVLKQDQATAVKNAEKFLKTVNMLDKKDSYPSMLSGGQQQRVAIARGLAMNPEML
                     LFDEPTSALDPEMIGDVLDVMKKLAHDGMSMIVVTHEMGFAKEVADRVIFMADGEVLE
                     DSRNVRDFFENPNEVRAQQFISKVINH"
     gene            13751..14566
                     /locus_tag="IXT20_001854"
     CDS             13751..14566
                     /locus_tag="IXT20_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HAQ4860292.1"
                     /translation="MRRLKKIRLIFLLGIAFLALAGCKGNGLANEDILERSKSTNEII
                     WGVKYDTRLFGMMDIESRTVQGFDVDIAKAITKKILGDNGKTEFVEVTSKTRIPLLKN
                     GNIDAIIATMTITDERKKQVDFSDVYFDAGQALLVKKGSQIKSVDDLNASTTVLAVKG
                     STSAANIRQHAPDAKILELENYAEAFTALQSGQGDAMTTDNAILLGIADENPEYELVG
                     GTFTNEPYGIAINKGQENFLKAVNQALEEMHADGTYDKIYQKWFPNETEGKVE"
     gene            14579..15226
                     /locus_tag="IXT20_001855"
     CDS             14579..15226
                     /locus_tag="IXT20_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAQ4860293.1"
                     /translation="MSELIQTYGPIFVDGFKITLFSSVLALVFSLIIGTLMAIFQLSK
                     NKVVSALAKAYVEFFRNIPLLIIVMFFYVVVPIYWFNLDGFQAGTIGLTIYTSAFIAE
                     TVRSGIQTVPKGQTEAGLSSGFTYSEIMRYIVLPQAFKIVIPPLGNQFINLVKNSSIL
                     AIVAGLDLMYQGDLIASETFNTFDTYIIVGLFYLVITLPLSYLMVYLEKKWAVRS"
     gene            15242..15898
                     /locus_tag="IXT20_001856"
     CDS             15242..15898
                     /locus_tag="IXT20_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAQ4860294.1"
                     /translation="MDFSGAFSWMNIRFLLEGLKVTVEVSLFSIIFSFLIGGLTGVIR
                     FANIPFFSKILGVVIDIIRNLPLLLIIFFTYFALPQIGIQMNIFWSAVAALTIFESAM
                     LSEIFRAGLNAVPKGQMEAGLSTGLTYVESMRTIVLPQAFKSMIPAIVSQLISLIKDT
                     SLAVIISLPELSHQARIIYGQNTNYVLPMFIIMTLMYFVVCYALSLLSGYLEKRRYSY
                     "
     gene            16060..16881
                     /gene="racE"
                     /locus_tag="IXT20_001857"
     CDS             16060..16881
                     /gene="racE"
                     /locus_tag="IXT20_001857"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HAQ4860295.1"
                     /translation="MIRLTDNRPIGFIDSGVGGLTVVKEALKQLPNENILFVGDTARC
                     PYGPRPAEQVIQYTWEMTDYLVEQGIKMLVIACNTATAVALEEIKAALSIPVIGVILP
                     GTRAAVKKTQNKQVGIIGTIGTVKSQAYEKALKEKVPELTVTSLACPKFVSVVESNEY
                     HSSVAKKIVAETLAPLTTKKIDTLILGCTHYPLLRPIIQNVMGENVQLIDSGAETVGE
                     VSMLLDYFNLSNSPQNGRTLCQFYTTGSAKLFEEIAEDWLGIGHLNVEHIELGGK"
     gene            16884..18239
                     /gene="rph"
                     /locus_tag="IXT20_001858"
     CDS             16884..18239
                     /gene="rph"
                     /locus_tag="IXT20_001858"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HAQ4860296.1"
                     /translation="MRHDGRKAQELRKLELQTNVFKYPEGSVVIRFGDTTVICSATIE
                     NSVPPFLRETGTGWVTAEYSMLPRATSTRNRRESSKGKLSGRTMEIQRLIGRSLRAVV
                     DLEKLGERSIIVDCDVIQADGGTRTASITGAFVALKLAIEKLLREKELSEDPIKEHLA
                     AVSVGILPDGTCVTDLDYQEDSAALVDMNLVMTESGKFVEIQGTGEEATFDGEQLNEM
                     LFFGKNAIEDLIKEQKHALLTEFAQNDERIEETKTIIIATRNPGKAEEFRNMFKEAGY
                     HVKTLLDYPELPDVEETGSTFEENARLKAETIAQLLDQPVLADDSGLKVDALGGMPGI
                     YSARFAGEQKSDAGNNAKLLYELTDVPDEKRTAQFHCTLVFAAPKKDSLVVEAEWPGR
                     VARIPSGENGFGYDPLFIPEGKKQTAAELSSEEKNKISHRAQAMKKLSAEWKQWLEGE
                     R"
     gene            18240..18758
                     /locus_tag="IXT20_001859"
     CDS             18240..18758
                     /locus_tag="IXT20_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HAQ4860297.1"
                     /translation="MRYLVVSDNHGDRDILVDLVDRYRSEVDIFFHCGDSELEASDPL
                     WDTFQVVQGNCDYGPGFEQKKVIQTGQDTVFMTHGHLSNVRFGLTQLAIEARAATADM
                     VFFGHTHQIGCEMDQKILFLNPGSISQPRGPIQIPSYALIESLDDQISVQYYNRAHQA
                     VEELAFSFSRER"
     gene            18865..19365
                     /locus_tag="IXT20_001860"
     CDS             18865..19365
                     /locus_tag="IXT20_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HAQ4860298.1"
                     /translation="MIGPIVREMLLENQETFLIPAENVANVMYHHPLSHGLLVLSKVG
                     YSKIPVLGKDDHFVGLVSLSDVVNKMIDLQTISMDPLEGLTVADVTETDVPTIDENWE
                     LEEVLHLLVDSPFLPVVTEDRIFKGIITRKELLKAVNFMVHELERRNILSPKIEELKE
                     QIDIVS"
BASE COUNT         6537 a         3340 c         4177 g         5398 t
ORIGIN      
        1 tttttgcgta tacttaaaaa agtagaaata tcagaacctt aaaaggatat aatttgaaga
       61 atatagaaca aatgttcagt attttttgat ataataaagg agcaccgtat catgtgttga
      121 aggaggaaaa gcatgtcttt acaatttatt cttggaaacg ggaaaaaaga tcaccgtcaa
      181 gtcttgatcg acgaagcatc cgcttggctc gaccaggatg caagtaacca agtatttttc
      241 cttgttccca actacagcaa attcgaacag gaacaagaaa ttttagcgga gatgcgaaga
      301 agaagcggaa aaaaaacttt cagcacaaca aacgttcaag tatttagttt ttatcgtctg
      361 gcttggtatt tgctgcaaca gacaactctt cttacaggaa atgaattatc agaatcagga
      421 tctgcaatga tcttgcgaca gattttagaa aaaaatgagg aagaattaac aatctttcgc
      481 ggagaaatca ataaaagagg gtttattcgt caattacaag aactctacag tgaattgcgt
      541 acaggaaata ttcaaccaga agacctgatg cttttatttg gtgattcacc taatgcagaa
      601 gaccagcaac ttaaaatgaa agacttgaag ctgatttttt cagcttatga cagcgaattg
      661 actcaacgtg cactaacaaa tgaagatgca ctatctatac tagcaaatta tatgcagaca
      721 caagatttca acaacgtcaa atttattgtc agtggttttt ctcgaatcaa tggtcaagag
      781 tatcaattac tgcaggcaat gatggaaaag gggcaattgg ttgtcgattt attgctggat
      841 cgtccttata cagcggatct tccagaagta cttacgctct ttcatgagac tggaaagctt
      901 tatttccgtc tttttcaaag tgcaagagaa cggaaagtcc ctatattagt ggaccgattt
      961 gcaccagagc agactcgtcc gacagagctt caactgctgc aaacaatgtg ggaagagaca
     1021 tctagccaaa agaaagcggc agtacacaaa atagggggac aagagcatct ccatttgtgg
     1081 gaagctgaaa atccaactga agaagtccga caattagctg ttgaaatcag acgactagtc
     1141 agtgaagaaa actaccgtta tcgtgatatc caagtgctga caaaaaatat gtctttatat
     1201 ggaaatatcc ttcggccgat ctttcaagaa atggcaattc ctttttatat cgatgaagaa
     1261 caaatgatgg aaaatcatcc gctgatcgaa tggatcaaca gtctgttcgc tcttgaccgc
     1321 tattcttatc ggctaagtga tattatgcgt tttttcaaaa ctgaattatt tctgtcgacg
     1381 gaagcagaaa ccgaacttga tgaatggaaa caagagcgag atgaattcag gaaaaaactg
     1441 gacatcactg aaaatgtagc acttgcttac aattatcaag gaacttattg gactcgtgaa
     1501 aaagactggc agtttgtggc gtatgatttt gaagcagaac agattgaaga cgtacagtat
     1561 ttggaagaac agtcaaatac gattagacga ttatttcaga aagctgtacc agtatttttg
     1621 aaaaaaataa agaaaagcaa aacaggacaa gaggccgcaa atgtttttta tcaatttttg
     1681 atccaaagtg gcgtacagca acaattattg ttttggcgta atcaggaagt agacagagga
     1741 aacttagaag cggcaagaaa ccatgagcag acatggggag cgctgattga tctgttggat
     1801 gaatatgtag taatttatgg tgaatcggct tttgactggt cactgttcca agaaatcatt
     1861 acaagtggtc tagaaaatct aagttatggt aaaatcccta ccgcaatcga ccaagtgcga
     1921 atcaatcgtt tggaattagt tcgaccaaat caagctgccg tcacttttgc attagggttg
     1981 aatgatcaag tgtttccaga ccgaaaagag acaaaaggat tgttgtccag tgaagaacga
     2041 gaagtattaa atcaggcctt gccagatgga cagtttttat tcgacccatc aaaggaaagt
     2101 atctcttttg agcctttcca agcctatctt gtttttcttt ccggaacaga tcgattatat
     2161 cttagttatg cacaaagcta tgacacagat ggttcgctga agatgtctcc ttatttgcgt
     2221 agaatcacag aatatttatc tgttcccgtc gagagaaaag agcatttgac gatcgaaagc
     2281 gaccctaatt gttatgtagg gacttacaga agcggaatca atacagtcaa taggatttat
     2341 cgattagctt tagatgaaaa aaaacctgta ccaaagcact ggcaaaagct caaagaatta
     2401 gtactgaatt cgtcatggcg aaattttgct ttacagattt ttgaaagtca ggaacattta
     2461 aatgttcctg tttcgatgcc agaaaaaatg gcggaagagc tttatggaaa agagatttat
     2521 acttcaattt cacgtatgga gaatttttat aattgtgaat acaaatactt catgcaattt
     2581 ggtttgaaat tgaaagaacg aactgtttat ggtctaactc cagcagcaac tggtgatttt
     2641 tatcatgaat cattggatcg tttcttcaag ctactgtttt caaaccaatt atccttagtg
     2701 aatatgtccg atgcgcagcg tagagacttt actgaaaaag tgctgcagga agtattcgga
     2761 gaacttcggt ttgagatatt ggatagttct gcaagaatga actatatccg ctaccagtta
     2821 ggacagacga ttcaaaaagt gacatgggct ttgcaaaaac agagcaaaaa tacaggcatg
     2881 catcctcttc agacagagat tctatttggt cagattgctg gagcaaaagg tatcacaggt
     2941 ctggaattac cgttgagtaa tggtggaaaa ctccatgttc gagggaaaat cgatcgaatc
     3001 gatgtagctt ctgaacaaga agatacttgg ctaagcgtcg ttgattacaa atctagcgga
     3061 cgttcattcg atgtcacaga agcttattat ggcatggcta tgcaattatt gacgtatctg
     3121 gacgtagcat tgatggatgc ggtacgtttg actggaaagg cggctgttaa accagcagga
     3181 tcatattatt tacatgttca taatcctgta ttgacggaga cagatgatgt agagaagaat
     3241 acattgaaaa aatttagtta tgatggcgtt tttgtcgatg atcccgctct tttagaagta
     3301 ctagatcatt cactggaagc aaaagaacaa tcactgatct atccagtgaa aaaaaatgcg
     3361 aaagatgaat accaaaaagg tagtttcagt aaagacaaat ttttcacgga agaagaactg
     3421 caactattca tggcgcataa tcgggaaaat atgcggttag ctggagatgc gattacttcc
     3481 ggagaaatca aactaaatcc agcttataaa gataaacagc ggattgcctg cgaattttgt
     3541 ccattcagaa gcatctgtac gtttgatgtg atgctgaaag aaaataatta ccaccgtctg
     3601 gagaaaatag ataaggaaga agcactgcgt agaatattga aaaggagtga agaagagtga
     3661 atcaaattcc tttaaaaccg caaaatgaac gattcacaga tgatcagtgg caagcgattt
     3721 ttgatcaagg agataatcta ttagtctctg cgtctgccgg atcaggtaaa actacagtgc
     3781 ttgttcgaag agtgattgaa aaactaaaaa tgggttttga tatcgatgaa ttattgattg
     3841 tgacatttac tgaagctgcc gcaagagaaa tgaaagaaag aattcaagag gcattacaag
     3901 aatccgtcaa tagcgaaagt gaccctgtac gtagacagca ttttactaag cagttagttt
     3961 tattgcccac agcgaatatc tcaaccttac atgctttttg cctgacagtt attcgacgtt
     4021 attattactt aattgatatt gatccagtct ttcgcatgtt aacagatgaa acagaaacga
     4081 tcttgatgaa ggaggatgtg tgggacgagc tgagagaagc actttatgca gaaaatgatg
     4141 aacgattttt ccaattaaca atgaattttt ctaatgatcg ttcagatgac ggcttgacga
     4201 atctggtgtt ttctctttat gagtttgccc gtgccaaccc tgacccccaa aaatggctag
     4261 aacagttaag cgataattat cgtttgcctg aggggctagc gaaatctcga ttatatcaaa
     4321 aacaaatccg tcctttagtt ttggcagata tataccaatg tgtacagtta tatgaacaga
     4381 tgactcagtt agcacaaggt gaaggattag aaaaaatgca tgagcaagta gctggagaac
     4441 aacagcagat caagaatatc tatgaagcgt tctcgcaaga tcggttagaa gaagcttatg
     4501 ctggtttgga acaactaact ttttcaactt ttaagagcag tcggaaagca gaattgaaag
     4561 aaatttccaa tgaagtcaag ggcatgaggg ataaagcaaa aaaactgatt cagcagataa
     4621 gtaaaagtta ttttcctgtt tccccaagcc aaatggaaga attaacggac aaagcactac
     4681 cgttagtcga agaaatgaca aaagtcacgc aaagcttcat ggacggtttc agtatgcgta
     4741 aacgggaaaa aggtgtgctg gatttcaatg atctggaaca tttagcgttg cagattctca
     4801 cagaaaaaac gaaagatgta tggctgccaa gtgaagcttc aaaacattat cgaaaaaaat
     4861 tcaaagaagt aatggtagat gaatatcaag acgtgaacca attacaagaa gccattttat
     4921 actggctaag agaacccgat gatacaaaag gaaatatgtt catggttggg gatgtcaaac
     4981 aatccatcta ttctttccgt ttagcagatc ctagtttatt tatcgggaaa tacgagaatt
     5041 tctcaaagaa agaaggcgga cgtcgaatcg ttttggctga gaacttccgc tctagaaaag
     5101 aagtcctttc ttttacgaat cttatttttg aacaactaat ggatccagca gtgggtcaaa
     5161 tcaattatga cgaagcagcc aaactgatcc aaggattttc agactttcca gagaatgaac
     5221 agtttgagcc ggaaatcatg atctatgaaa aagaacagga agaatcagag attgagattc
     5281 cgacggatga tatactggaa gacaaaacag aaggcgaact ttttatgaca ggactaaaaa
     5341 ttcgtcaatt aattgattcc tcctttatga tttatgataa aaaatcaaaa aaaagtcgtc
     5401 cgatagagta caaagacatt gttctcttga cacctacaaa gaagaacaac ttaacgattt
     5461 tagagatttt caaaacattg gatattccat tagagatgaa tgatgctcaa aactattttc
     5521 aagcgacaga aattcgcacg atgatctcgc tgctacaatt aatcgataat ccctatcaag
     5581 atattccgct tgcggctgta ttgcgctcac cgatcgtggg tctgattgaa ccagaattag
     5641 ccagcattcg attagctaac cgtgcacata cttactatga tgctgtgctt gcttatcaag
     5701 catcaaacga agatgaatta gcagctaagc tggaacattt tggcaagcaa ctagagcatt
     5761 ggcgagaact tgctcgccgt tcttcgatca ctgacttgtt atgggatatc tattatgaga
     5821 cgggatatct agagtatgtg gtcggtctgc cagcaggggc acagagacaa gccaatcttt
     5881 atgcattggt tgatcgtgcc aaagcttacg agcaaagttc tttcagagga ttataccagt
     5941 tcgtgcgttt cattgaaaaa atgcaagaaa aagataaaga cttagcagaa ccagtcattt
     6001 caatagaaga taatgctgtt cgcgttatga cgatccatgc aagtaaaggc ttggaattcc
     6061 cagttgtatt tttgttagat atgacaaagg agttcaactt acaagacttg cgcaatcggt
     6121 atgcttttga agaaaaactt ggagcaggaa ttcgctatat ggatcctgaa acacgagtgt
     6181 tatatgatac gttaccgttt caagccatca aattggcaaa gcaaaacaaa ttgttgtcgg
     6241 aagaaatgcg taaattgtat gtaggattga ctcgggcaga acaaaagctt ttcattgtcg
     6301 gttcctataa aaataaagag caaatgatcc aaacgtggtc tgaagcagct gatcatgagg
     6361 agttagtttt tgatcctgcg ctacgtttga agggcagaag cagcttgatg aattggattg
     6421 gttacggatt gatccgtcat cctgaaatgc agaaatactt agaggaagaa atttcgactt
     6481 ctcttcttca acattcaaat gcacagtttt ctatttcatg gatgaatcag caatcgatca
     6541 ttgaacaaag acagttgctt gctgaaaaag aactggttaa cttagatcag cagatgaaag
     6601 aagatgaaac tttattagca gattcgttac aaaaacgtct ggcatatgaa tatccttatc
     6661 aagcttcttc acaaaccaca agttatcaat ctgtatctga aatcaaacga ttattcgaag
     6721 atccagatga tacacaagaa tcacgtttga ctctggaaag cagtcagaac aaagcagcat
     6781 cccgtcaatt tcgttatacg caagaacaac tagcagaacc aaaatttttg caaaaagatc
     6841 ggcaagtgtc agcagctgct gttggaactg ctacgcatgc tttgttacaa ttactgccat
     6901 tagagatgcc aactacagag agtatccatc aaaaattaca ggaattggta aggaaacggt
     6961 tagtagacga aaaagtcgcg aaaaaagtag atgtttcttc tattatatgg ttctttcaaa
     7021 cagaacttgg tcaacagctg attgccaaca aagaaaacgt aaaacgagaa caaccttttt
     7081 ccatgctgct tcctgctgac gaagtatttc aagactatcc aaatcaagaa gatgaactat
     7141 taatccacgg aatcgtcgat ggatacctag aagaaaaaga tcatctcaat atctatgatt
     7201 ttaaaacaga ttttatttta catccggatg atccagcaga aatagatgca atcgtccaaa
     7261 aatatcaagg acagctaaga ctttaccaac aagcaatgtc agaagctttg aataagccag
     7321 tagaaaatgt tttcctaatt ttgcttcgag tgaaacaaat tataaacatt aacaaataat
     7381 tataattgag caaacttgac ttatgttttg taaggattat gctatactct gggaagaggt
     7441 gagtatgatg gagctaacac agacagaatt cgttttatgc cctaaatttg aaaaaacatt
     7501 ctctatttta ggaaaaaaat ggaacggatt gattatcgat gtactactcg aaaacggtcc
     7561 gcaacgcttt gttgagcttg cggcaaaaat cccgatggtc agtgatcgag tattagtcga
     7621 acgcttgaag gaattagaaa aagaaggaat agtcactcga aactttgcct gtagcgaaaa
     7681 gagccgttca gaatatctct tgactgaaaa aggagcagac ttgcaaaaag cgatgcgtca
     7741 aatacaagtt tggagcgaaa aatggatcac agaggaagaa tgcggttgat tttgtaaaaa
     7801 aaatcttgta aactatagtc catacgaata agtaaagaac gatgacgaaa aagagtagtt
     7861 tggctaaacc agttcaggga gatctgtcgc agactgaaag cagatcctgt gtttgaaaaa
     7921 tgaagttcac tttccgagtt cgtctgataa aatcagaccg gcaattgtcg ttaacgaagt
     7981 aagagtgcgg tgtaagtcat ttatccgcaa attaggatgg taacgcgaag acctcgttcc
     8041 ttaacaggaa tgaggtcttt ttgttttgta tttggaattt aaaggaggaa caagcacatg
     8101 tctctacaag cacaattaga gagcttgaaa gaagcaacgc tcgtcaaaat cacagcggca
     8161 caagatttaa atgaattaaa tcaaatacgt gttgaaacgt taggaaaaaa aggaccgatc
     8221 acagaagtat tacgtgggat gaaagatcta agtccagaag agcgaccaaa agtcggaagt
     8281 ttcgcaaatg agatccgaga tcttttgaca gaagagatcg tgcgaaaaaa acaagtatta
     8341 gaagaagaag cgttaaatgc agctttggca aaagaaacaa tagacgtgac actacctggt
     8401 agaaaagtaa aacatggtac acgtcatatc gtgacacaaa tcatggaaga aattgaagat
     8461 atttttgtgg gtatgggcta tcagatcgta gaaggatatg aagtagaatc tgatcattac
     8521 aatttcgaac ggatgaatct gccaaaagat catccagcaa gggacatgca agatactttc
     8581 tatatttcag acgagatatt gattcggaca catacttcac cagtccaagc acggacaatg
     8641 gaaaaacatg atttctctaa gggtgctcta cgaatgatct cgccagggaa agtcttccgc
     8701 agagatacag atgatgcgac ccacagtcat cagttccatc aaattgaagg gctagttgtt
     8761 gataaaaaca tcacaatggg cgaccttaaa ggaacattag aagtcgtaat gaaaaaaatg
     8821 tttggggaag atcgtgaaat tcgtttgcgt ccaagttatt tcccatttac ggaaccatca
     8881 gtagaagtag atgtcagctg tttcaaatgt ggtggtgccg gttgtaacgt atgtaaatac
     8941 actggatgga tcgaaatttt aggagctggc atggtacacc cgaatgtgtt gaagatgtca
     9001 ggaatcgatc cagaagaata ttcaggcttt gctttcggcc ttggaccaga ccgcgtagct
     9061 atgttgcggt atggagtgaa tgatatccgt aatttctatc aaaatgactt acgtttctta
     9121 agtcagttca agggggagta agaaaatgtt agtttcatat aaatggctca atcgttatgt
     9181 agatttatcg aatgtaacac caaaagaatt agcggataag atgtctgtga caggaatcga
     9241 agtagaggga gtaacggttc cagaagaagg cttgaaaaaa atcgttgtcg gtgaagtcaa
     9301 ggaatgcgta ccacatccag attctgatca cctttctatt tgtcaagtcg atattggcga
     9361 agacgaactg tctcaaatcg tttgtggagc gcctaatgtg aaggttggaa tcaaagtgat
     9421 tgttgcactt cctaactcac gtatcgctgg taatgtaaaa atcaaaaaag gaaaaatgcg
     9481 tggccaagta tcaaatggca tgatttgctc attacaagaa attggctatt cagatagtgt
     9541 tataccgaaa gaatacgctg aaggtatcta ttatatgcca caagaagctg taaatggcga
     9601 tccagttttt ccttatttgg atatggatga ttcgattatt gaattatcta tcacaccgaa
     9661 tcgtgcggat gcattgtcaa tgcgtggcgt agcttatgaa gttggggcaa tttatcgtca
     9721 gactcctaca ttcgatgacg aaaagctggt agaatcctca cgtcctgcta gcgataaaat
     9781 tcaagtggaa gttgaagata aagaagcagt tcctgcttat caaatcagaa tcattgaagg
     9841 agtaagaatc cagccaagtc ctcaatggct gcaaaatctt ttgatgaacg aaggcattcg
     9901 tccaatcaac aatgtagtag atgtgacgaa ctatattttg cttttattcg gacaaccact
     9961 acatgcattt gactacgaca aattgaaatc aaaagagatc tttgtccgcc gtgcaaaaga
    10021 aaatgaaaca ttagtaacac tagatggaga atctcgggaa ttgacaactg acaatctcgt
    10081 cattactaac ggcaaagaac cagttgcttt agctggtgtc atgggagggt tagactcaga
    10141 gatcacgaat gaaacaacaa cagttgcact agaatctgct ttatttgatc caatttcgat
    10201 tcgtcgtaca gcaaaacaat ttaatttgcg tagtgaatcc tctgcacgtt ttgaaaaagg
    10261 aatcaaccat gcgactgttg gagaagccag tgaggttgct gcagcaatga tcgtttctct
    10321 tgcgggtggg gaagttctac aaggcgcggt caaaggctca gagatcaaag cagaagacgt
    10381 atcagtgaag atttcacttg aacgcatcaa tcaatattta ggaacatctc ttgtcgttaa
    10441 agaagtaaat gagatttttg aagcacttgg atttagctat gaagaaaagg agtatgttta
    10501 ttccgtagta gtaccaccaa gacgctggga tatccaaatc gaagcagata ttatcgaaga
    10561 agttgctcgt atttatggat acgaccagtt gccatctaca cttccaagtg gagaaacggt
    10621 tgctggaagc ttgtcaaaag aacaagaaac tactcgtaaa attcgtacaa tctttgaagg
    10681 aagcggattg agcgaagcaa tcagttatgc attgacaaca gaagaaaaat caagacagtt
    10741 tgttataaat gaaagtaata ttacacgttt ggactggcca atgagcgaag aacgttctgt
    10801 attacgaatg aatttgatca gcggactatt agatgatatc agttataaca ctgcccgaaa
    10861 aaatacagag attgctttat atgaaattgg tcgggtgttc ttccaagaaa atgatccacg
    10921 tacacatctg ccgcaagaag taaaacatgc agcaatcgct atttctggta tttgggaaga
    10981 aaaagattgg cagacgaaag ctcaaaatgt agactttttc accatcaagg gtcttttaga
    11041 aaacttattt gaacaactag gcatcactga agatgtccat tatcaaacag tgacagaaat
    11101 gaaggaaatg catcctggaa gaactgctgc gatttacctt ggcgaaaaat ttattggttt
    11161 tgtcggtcaa gtgcatccaa cgattacaaa aagttatgat attccagaaa catatgttgc
    11221 tgagtttgat ttatctgcta tagtggaagc tgctcaaaaa gggatcgttt tcgaagcagt
    11281 gacaaaattc ccagcagtca gccgggacat cgctttgctt gtaaaagaaa ctgtgacaaa
    11341 tcaggaatta acagaagtta tccgttctgc tgccggaaga tttttaacag atatccaatt
    11401 atttgatgtc tatcaaggag aaaacattga aaaaggacat aaatccatgg cgtatagcct
    11461 gacattcgtc aacccagaag caacattgac agatgaagaa atcaataaag caatggaaaa
    11521 agtcgaaaaa gcgttggtag aaaacctaga ggcaagtatt cgctaagaag gaaaatatag
    11581 taagtaacga tcaagtagct tttgaagacc atacatttga aaaaaagatg tgacacaact
    11641 tcgtcacatc tctttttttg attttaaaaa aggaatagca cagagacgag ataaaaaaac
    11701 aggcctttac agcctgcttt tttagagtaa atgaattaat ggaagtaatt gattcccatt
    11761 gctgatttta cttcagaaag tgtttgtgca gctactttag cagcttcttc actaccttta
    11821 cgtaagattt ccataacagc tgaaggatct ttttccaatt cttctcggcg cgcacggatc
    11881 ggtgcaaatt cagcttccaa tacatcaatg aggtatcgtt tgatctttac atcaccaagt
    11941 ccgccgcggc ggtaatgagc tttcatttct tcaattgctt ctttatctgt tccgaataca
    12001 tccaagtagg tgaagaccat attcccttct acttgtccag gatcttgtac atggatgtgg
    12061 tttggatcag tatacatact catgactttt ttctgtaaga catcggcagg atcggagata
    12121 taaataccgt ttcctaacga tttactcatt ttgccatttc catcgattcc aggtaatcta
    12181 cccattcctt ttggtgggaa tacgcctttt ggttccacca agacttcacc atatgtatga
    12241 ttgaagcttt gtacgatttc ctgtgtctgc tctagcattg gtttttgatc ctcaccaaca
    12301 ggtacaagat tcgctttgaa tgcagtgata tctgctgctt gagaaacagg atagataaag
    12361 aatcctgtag gcacgctttc accgaatttt ttttgttcga tttctgtttt gacagtcgga
    12421 ttgcgccgaa cgcgtcctac gctgactaag ttcaaataat acatggttaa ttcagctaat
    12481 tccggaattt gtgattggat aaataaagta gattttgcag gatctaatcc cacggctaaa
    12541 tagtctaaag caacttgcaa gacatttgat gaaaccttct caggattttt agcattatct
    12601 gtcagtgctt gcatgtcagc aatcatgaca aacagttggt tgtcttcatc tgcctgcata
    12661 gccactcggg tttttagaga accgacgtaa tgccctaaat gcagcttccc agttggtcga
    12721 tctcccgtta aaataatatt tttcaaaaaa gacacctctt tacgatttgt aagtctgagc
    12781 acttaatatc ttaccgtttt tcctgaaaag aaacaacagt tcctaaggaa aaaacaagga
    12841 aagaggataa aataactaat agattagaaa tggaagaaat atttatggga gaataaacgt
    12901 tctaagaaac tttacaaatt actaaactat cagatataat gtattttatt atacatcttt
    12961 aaaagaaatc ttactaaata ataatgggag tgtaattcta tggcaatgat taaatttgat
    13021 cacgtggata aatattatgg caagttccac gcattaaaaa atatcaatct cgaattcgag
    13081 aaaggagaag ttgtcgttgt cattggtcct tctggttcag gaaagagcac gatgcttcgc
    13141 tgtatcaatg gattagagac gatctcttca ggtaagctgc tgattaatga tactgacttg
    13201 catgataaaa agacaaaact gacagaagtc cgaaaaaaca tcggcatggt tttccaacat
    13261 ttcaatttgt acccaaataa aactgttctg gaaaacatca aattagcacc aatcaaagtg
    13321 ttgaagcaag accaggcaac tgcagtcaaa aacgcggaga aatttttgaa aactgtcaat
    13381 atgttagata agaaagattc ttatccttcc atgttatccg ggggacaaca gcaacgggtg
    13441 gcaatcgcca gaggtttagc gatgaatcct gagatgctgc tgtttgatga acctacttct
    13501 gctttggatc cggaaatgat tggtgatgta ttagacgtta tgaaaaaatt ggctcatgac
    13561 ggtatgtcaa tgattgtcgt gacacatgaa atgggctttg ccaaagaagt ggcggatcgt
    13621 gttatcttca tggctgatgg agaagtattg gaagacagca gaaatgtgag agactttttc
    13681 gaaaatccaa atgaagtccg agcacagcaa tttatcagta aagttatcaa tcattgatag
    13741 gaggaaaaag atgcgcaggt taaaaaaaat acgcctgatt ttccttttag gcattgcttt
    13801 tcttgcacta gccggatgta aaggaaatgg gttggcaaat gaagatattt tagagcgaag
    13861 caaatctacc aatgaaatca tctggggtgt caagtatgat actcgattat ttggaatgat
    13921 ggatattgag agcagaactg tacaaggatt tgatgtagat attgcaaaag caatcactaa
    13981 aaagatcctt ggtgacaatg gaaaaacaga atttgttgaa gtcacttcta aaactcggat
    14041 tccattattg aaaaacggaa atatcgatgc aattattgca acgatgacga tcacagatga
    14101 acgaaaaaaa caggttgact tttcagatgt ctatttcgat gctggccaag ctttgttggt
    14161 aaagaaagga agccaaatca aaagtgtaga tgatttgaat gcttctacaa cggttctagc
    14221 agtcaaaggt tctacatcgg ctgcgaatat ccgacagcat gcaccagatg caaaaatttt
    14281 agaattagaa aactatgcag aagcatttac tgctttacaa tctggtcagg gagacgcgat
    14341 gactacggat aatgcgattt tgttagggat tgcagatgaa aatccagagt atgagttagt
    14401 tggtggaacg tttactaatg agccttacgg gattgcgatc aacaaagggc aagaaaactt
    14461 cttgaaagct gttaaccaag cgctagagga aatgcatgca gatggcacat acgataaaat
    14521 ctatcaaaaa tggtttccaa atgaaactga aggaaaagta gagtaagggg gagaggcaat
    14581 gagcgaattg atacaaactt atggaccaat ctttgtcgac gggttcaaga tcacattgtt
    14641 ttcgagtgtc ttagcacttg tgtttagttt gattatcgga acgttaatgg caatcttcca
    14701 attatcaaag aataaagtag tgagcgcctt agcaaaggca tatgtagaat tttttagaaa
    14761 cattccatta ttaatcatcg tgatgttttt ttatgttgtc gtaccgattt attggtttaa
    14821 tctagatgga ttccaagcag gtacgattgg attaacgatc tatacatctg cttttattgc
    14881 tgaaacagta cgttcaggga ttcaaaccgt accaaaaggt caaacagaag caggactatc
    14941 ttctggtttt acctattcag aaatcatgcg ttatattgtt ttaccgcaag cattcaaaat
    15001 cgttatcccg ccattaggca accaatttat caacctcgta aaaaactcat ctattttagc
    15061 aatcgtagct ggtttagatt tgatgtatca aggagacttg atcgctagtg aaacattcaa
    15121 taccttcgat acgtatatca tcgttgggtt attttactta gttatcacgc taccgttatc
    15181 ttacttaatg gtttatcttg agaaaaaatg ggcggttcgt tcatagaagg aggggaaata
    15241 catggatttt agcggcgctt tttcatggat gaatatccgt tttttattgg aaggattaaa
    15301 ggttactgtt gaagtttcat tgttttcgat catttttagt ttcttgatcg gtggtctgac
    15361 aggtgtgatt cgttttgcga atatcccttt cttttccaaa atccttggtg tagtgattga
    15421 tattatacgg aatcttccat tattattgat cattttcttc acttactttg ccttacctca
    15481 aatcggcatt cagatgaaca tcttctggtc cgctgtcgca gcattaacga tttttgagtc
    15541 agctatgttg tcggaaattt tccgagctgg actcaatgct gttccaaaag gacagatgga
    15601 agcaggatta tcaacgggac tgacttatgt agaatcgatg cgaacgatcg tgttaccgca
    15661 agcatttaaa tcaatgatcc cggcaattgt gagccaattg atttcactga tcaaagatac
    15721 ttcattagca gtaatcattt ctttacctga actttctcat caggcacgta ttatttatgg
    15781 acagaacacc aattatgtgc ttccgatgtt catcatcatg acacttatgt acttcgtggt
    15841 atgttacgcg ctttctctgc tttccggtta cttggaaaag agacggtaca gttattaata
    15901 gatgattctt ttagcggttg tggcaaacta aagtcacaac cgcttttcct ctttccattc
    15961 gaaaaaaaga gatttcgatt gttttaagca agtaagagca cttgaaaatt cacgctaaaa
    16021 agtacacaat agggaggata gaatttattg aaaaaaagga tgatacgatt gacagataat
    16081 cgccctatcg gatttattga ttcaggtgtc ggcggcttga ctgtagtaaa agaagccctg
    16141 aaacaattac cgaatgaaaa tattttattt gtaggagaca cagcacgctg cccatatggc
    16201 cctagacccg cggaacaggt aatccagtat acttgggaaa tgacggatta tctggtggag
    16261 caaggaatca agatgctggt gatcgcctgc aataccgcaa ctgcggtggc tttagaagaa
    16321 atcaaagctg ctctttctat tccagtcatc ggtgtgatcc ttcccggtac tagagcggca
    16381 gtaaaaaaaa cacaaaataa acaagttggc attatcggta cgattggtac ggtaaaaagt
    16441 caagcttatg aaaaagcact gaaagagaaa gtaccagaat tgactgtgac aagtcttgct
    16501 tgtcccaaat ttgtttcagt tgtcgaaagt aatgaatacc attcatcggt ggcgaaaaaa
    16561 attgtggcag aaacattagc tcctttaacc actaaaaaaa tcgatacatt gattttggga
    16621 tgcacccatt atccattatt acgccccatc attcaaaatg taatgggaga aaatgttcaa
    16681 ctgatcgatt ctggagcaga aacagtaggt gaagtatcta tgctgttaga ttatttcaat
    16741 ttgagcaatt caccgcaaaa tggtcggaca ttatgccagt tttatacaac tggctctgcc
    16801 aaacttttcg aggaaatagc tgaagactgg cttggaatcg gacacttaaa tgtagaacat
    16861 atcgaattgg gaggaaaata aggatgcgtc atgacgggag aaaagcccaa gagcttcgca
    16921 aactggagct tcaaacaaat gttttcaaat atccagaagg atcggtagtg atccgttttg
    16981 gtgatacaac agttatttgt tctgcaacaa tagaaaattc tgttccccct tttttgagag
    17041 aaacaggaac aggatgggtg acagcagaat atagtatgct gcctcgagca acgagtacga
    17101 gaaatcgtag agaaagcagc aaagggaaac tttctggtag aacaatggaa atccagcgtc
    17161 ttattggtcg ttccttgcgt gcagtagtag acttggaaaa attaggagaa cgtagtatca
    17221 tcgtagactg cgatgtgatc caagcggatg gcggtactag aacagcaagt atcactgggg
    17281 catttgtggc attgaaatta gccattgaaa aacttttaag agaaaaagaa ttgtcagaag
    17341 atccaataaa agaacatctt gctgcagtta gcgtaggaat cttaccagat gggacttgcg
    17401 tcacagattt ggattaccaa gaagattccg cagcacttgt agacatgaat cttgtaatga
    17461 cagaatctgg aaaattcgtg gagattcaag gaactggaga agaagcaacg tttgatggtg
    17521 aacagttgaa tgaaatgctc ttttttggaa aaaacgcgat tgaagatttg atcaaagagc
    17581 aaaaacatgc tttgctaaca gagtttgctc aaaatgatga gcggatagaa gaaacaaaaa
    17641 caatcatcat tgcgacaaga aatccaggaa aagcagaaga gtttcgaaat atgttcaaag
    17701 aagcgggata tcatgtaaaa acattacttg actatcctga attgccagat gtggaagaaa
    17761 ctggcagcac atttgaagaa aatgcccgat taaaagcaga aacgattgca caattgcttg
    17821 atcagccagt cttagcagat gattcaggtt tgaaagtgga tgctttaggc ggtatgccag
    17881 gaatctattc tgcacgtttt gcaggggaac aaaaaagtga tgcggggaat aacgccaaat
    17941 tattatatga attgacagat gtgccggatg aaaaaagaac agcacaattc cattgtacgt
    18001 tagtattcgc ggcacccaaa aaagacagcc ttgtagtaga agcggaatgg cccggtcgag
    18061 tagcccgtat accatctggc gaaaatggct ttgggtatga tcctttattt atcccagaag
    18121 gtaagaaaca aacagcagca gaattatcga gcgaagaaaa aaacaagatc agtcatcggg
    18181 ctcaagcaat gaaaaaattg agtgccgagt ggaaacaatg gctggaagga gagagataga
    18241 tgcggtattt agtagtaagt gataatcatg gagacagaga tatcctcgtt gacttagtag
    18301 atcgttatcg ttctgaagtg gatatctttt tccattgtgg tgattcggaa ttagaagctt
    18361 ctgacccatt atgggataca ttccaagtgg ttcaaggtaa ttgtgattat ggaccaggat
    18421 tcgaacagaa aaaagtgatc caaactggtc aggatacagt attcatgact cacggtcatt
    18481 tatcgaatgt ccgatttggt ttgacacagt tagcaatcga agctcgagct gcaactgccg
    18541 atatggtgtt ttttggtcat acccatcaaa taggctgtga gatggatcag aaaatcctgt
    18601 ttttgaatcc tggtagcatt tctcagccta gaggcccgat ccaaatccca tcatatgctc
    18661 ttatcgaaag tttagatgac caaatttctg ttcaatacta taaccgtgcc catcaggcag
    18721 ttgaagagtt ggccttttct ttttcgagag agcgctgaat aaaaaaactc taatttttaa
    18781 taaaaataag ctttatttta tgaattttgc atccggtttc ggtaaaatag tagtgttgaa
    18841 tgaaatagaa atggaggcca tcagatgatt ggacctatag taagagaaat gctgctggaa
    18901 aatcaggaaa cgtttttgat tcctgcagaa aatgtggcaa atgttatgta tcatcatcca
    18961 ttgtcccatg gtttacttgt gttatcgaag gtcggttatt ccaaaattcc tgtattagga
    19021 aaagatgatc actttgtagg actagtaagt ttatcagatg tggtcaataa aatgattgac
    19081 ttgcaaacaa tcagcatgga cccgttagaa ggactaacgg ttgctgatgt gacagaaaca
    19141 gatgttccga caattgatga aaattgggaa ctagaagaag tactccattt gttagtcgat
    19201 tcgccttttt taccggttgt cacagaagat cggattttca aaggaatcat tacaagaaaa
    19261 gagctgctta aagccgtaaa cttcatggtg catgaattag aacgtcgtaa tatattgtct
    19321 ccgaaaatcg aagaattaaa agaacaaatc gatatcgtga gctaaagaac gagaaactaa
    19381 aagataatca aaaaataagt agaaaaaaca gcgtaccagt tttgacttta tcttgaaaaa
    19441 gaggctggga ca
//
LOCUS       DACGLE010000049        19274 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0534
            SAMEA3304449-rid9560453.denovo.053, whole genome shotgun sequence.
ACCESSION   DACGLE010000049 DACGLE010000000
VERSION     DACGLE010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304449
            Sequence Read Archive: ERR985064
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19274)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19274)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702225.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702225.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 20:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,045
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 27 of 67
            Pseudo Genes (incomplete)         :: 25 of 67
            Pseudo Genes (internal stop)      :: 19 of 67
            Pseudo Genes (multiple problems)  :: 4 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19274
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304449-rid9560453.denovo.053"
                     /strain="VREN0534"
                     /db_xref="taxon:1352"
     gene            complement(19..2703)
                     /locus_tag="IXT20_001861"
     CDS             complement(19..2703)
                     /locus_tag="IXT20_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4860299.1"
                     /translation="MKIIGKKIANRKINWLLISFLILIAMIIFTSNVAEGSIFNWFDD
                     TKSQTEFLTNPLYQNYLKKYNGNFYSQFGMWIGWSVIKSMFAVVDGIQNMIPEVLDLF
                     SFVESAGLNKVYQSVLSTIVVGLMILSLVIVGYKMIIGKGTVDLKSVGMNVVMSISLI
                     LLMPTLIHSGIEFSKTFYNDSTTITDSDEGVAWSLIEQGVTDLAYINKTDQYSMIDKT
                     NDRNNLTKENFWKTDLTQVLTDNVLDKMEKENPIADNLRYELVENSDNEFVATKFSDN
                     FLSTFVDGIKSGYYRYQANLWGISFGLIALAVAYVFSAFIIITAILELAFKRVLGVLV
                     FATDLETGQRSKQVLSDILQCYLTVGFQGFGLSMFAMFVNYLNAGEGISTNIFIKTIA
                     YICAVFVLIKGSGTVMRYFGVDIGLKEGYGQLASAFGMGAMLFRKGTGGMNRSKGGKN
                     SGGNDQAEGEERKPERNFGQSLSQKANKTGKTLGYARERGLSGLASDGATMASERATK
                     PFKSMRDIANDTKNKFKEGLDDGTVSAINKNSKPIVAKNKEDETGKYADSMPTRLSDR
                     KDGAKVENADRIMSSSERMREAMKNNAESNTNHASAIQQKVKQDIEERKNAMHGQAKS
                     AEELINQKRQEAKYTPEIMNREEMLRKRVEGRTGANMDEKEALTKERIQEAKNSNTGL
                     EKLVKENLQNSTSSQDRRSVDVRENLQNTTSSQSGNRIVDVRENVQSSASNQSRTVDV
                     RENLQNTTSSQSGNRTVNVRENVLSSGTSQPRTVDVRENLQNTTSSQSGNRTVDVREN
                     VQSSTSSQGFKTVDVRENVQKDSGEMKEKAQKINIVEDRKSAKKFDSNHETVIIDSEI
                     RENDKGSKSRRRFTYEDNELFRDTLNDPNPLFDNLLKK"
     gene            complement(2928..3806)
                     /locus_tag="IXT20_001862"
     CDS             complement(2928..3806)
                     /locus_tag="IXT20_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication protein DnaD"
                     /protein_id="HAQ4860300.1"
                     /translation="MKSMAEKRMLSKTFLNNASFMKMSDSAKLLYVYLNTNADDDGIV
                     EAYPYVKMLGTPDDDVKILFTKGFVFFLNEDLLAFLPHWLQNQNIRIDRYKPSIYRFK
                     LLNLKPEFFSKLKPIKLDVILKSGQVEPLRVDLMEKWYPVGIPSINHLEVQSSQDQIS
                     SDKSSLVKNSIDKINQVDTIINEEIINEKGIQLIHKLGDRFTKSQREKFVQLIVDMKN
                     STEREFKIRIDGPHYYEQTNKMIIRLFNTINEKLNLGKELNEMKYVNTVSRNHWKEIA
                     LEEQDNRMYIIDALSK"
     gene            complement(3951..4253)
                     /locus_tag="IXT20_001863"
     CDS             complement(3951..4253)
                     /locus_tag="IXT20_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ4860301.1"
                     /translation="MEAIKLMISEEQEEMFLKQFYQIATKAIEKAQENAGMTREYLNQ
                     KEMAEYLNVSENFLKNAVRNEGLPVVELGARKFYSKKAVNEFMFARMKRGESFERK"
     gene            4455..5252
                     /locus_tag="IXT20_001864"
     CDS             4455..5252
                     /locus_tag="IXT20_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ4860302.1"
                     /translation="MKPNPSEAGKRIKQLRLSCGFTMEELGRKIDNSPRATISNWERG
                     TNLPNPQKLKLLSTITNSTIDWIKWGTLEEYITSYLIDIGYELYIKDFPEIPHKVFKD
                     IQERYSDTFSLDKDYELLNPIIKNIFTKYYSKDFEIYLNEILANNINPLIEEYANQRH
                     SISKELFFKRANSKLQDKIRNGDFKYGEKERMIDSLKQLLDEMDLAYKDISKYSSVED
                     FFYKITKNQFETEKFISDLSKKYNFPYEKNSIIAKFLTDNHDKFKNN"
     gene            5303..6445
                     /locus_tag="IXT20_001865"
     CDS             5303..6445
                     /locus_tag="IXT20_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAQ4860303.1"
                     /translation="MATFFSYKLQNGEKYWGFETYLGKDPKTGKDIRTKRRRFKSKAE
                     AQTALNRLLVDFKEKQEFKINVTTFQELYELWIDNYRTRVKPSSVAVAQRFCKNHILP
                     AFGELRLDKISVSYCQKQVNEWHKTLKQYGFLRKTTAQIFKFGVAMEVCKDNPMSKTL
                     LPRKIEEEQPLKFYTKDQLQLFLQNTKENNSVKMYTFFRLLAYTGMRKSEALALQWED
                     IDYFNKTITIGKTIAQDEFNQVVLQVPKTKNSSRTIQLDDFTLKQLRIWQQEQMKIMI
                     LYGYNTNSPKQFLFTTNTNKLYYPQVVNDWLDWIYKKTPMEPQITPHGFRHTHCSLLF
                     ESGASIKEVQERLGHKDIKTTMNIYAHVTPQSVKKTGDRFAKFMGE"
     gene            complement(6543..7934)
                     /gene="sufB"
                     /locus_tag="IXT20_001866"
     CDS             complement(6543..7934)
                     /gene="sufB"
                     /locus_tag="IXT20_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HAQ4860304.1"
                     /translation="MGVPELEEYKFGFHDDVEPVFSTGEGLTEEVIREMSRIKGEPEW
                     MLEFRLKSLETFNKMPMQTWGPDLSDIDFDAIKYYQKPSDKPARDWEDVPEKIKDTFE
                     KIGIPEAERAYLAGASAQYESEVVYHNMKDEFEKLGIVFTDTDSALKEYPELFKEYFS
                     KLVPPTDNKLAALNSAVWSGGTFIYVPKGVKVDVPLQTYFRINAENTGQFERTLIIVD
                     EGASIHYVEGCTAPTYSSNSLHAAIVEIFTRKDAYCRYTTIQNWSDNVYNLVTKRAKA
                     YEGATVEWIDGNLGAKTTMKYPSVYLDGKGARGTMLSIAFAGANQIQDTGAKMIHNAP
                     NTSSSIVSKSISKDGGEVNYRGQVTFAKNSAGSISHIECDTIIMDEWSKSDTIPFNEI
                     HNSQVSLEHEAKVSKISEEQLYYLMSRGLTESEATEMIVMGFVEPFTKELPMEYAVEL
                     NRLISYEMEGSVG"
     gene            complement(7939..8409)
                     /locus_tag="IXT20_001867"
     CDS             complement(7939..8409)
                     /locus_tag="IXT20_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HAQ4860305.1"
                     /translation="MGLSKLDNLYRQVILDHSSHPHHRGSLTDSTSKIELNNPTCGDV
                     IELQVDVKDGIIKNIAFDGSGCSISTASASMMTDAVIGKSIEEAEHLTVDFLQLVQGK
                     EVKDPDQLGDAAMLGGVAKFPARIKCATLAWKALGKAMENDGTAAFHHYHKEEE"
     gene            complement(8396..9631)
                     /locus_tag="IXT20_001868"
     CDS             complement(8396..9631)
                     /locus_tag="IXT20_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HAQ4860306.1"
                     /translation="MIDAKKLRNDFPILFQDVNDEPLVYLDNAATTQKPKQVLQALAH
                     YYEHDNANVHRGVHTLAERATHDYEQAREKVRAFIHAKETAEVLFTRGTTTSLNWVAR
                     SFGDRYIKAGDEIVLSYMEHHSNVIPWQQLAKRTGAVLKYIELTEEGFLDMEDAAKKI
                     TDKTKIVSIAHVSNVLGVINPVQELAELVHENGGVIVVDGAQSAPHMTIDVQALDCDF
                     FAFSGHKMCGPTGIGVLYGKRKWLEEMEPVEFGGEMIDFVELYDSTWKELPWKFEAGT
                     PNIAGAIALGHAIDYLQELGMDNIHRYEEELAAYVLPKLQAIDGLTVYGPQDPNFRTG
                     VIAFNLDGLHPHDVATALDMEGVAVRAGHHCAQPLLKYLEVSATARASFYFYNTTQDA
                     DRLVEAIMATKEFFKHGTI"
     gene            complement(9628..10914)
                     /gene="sufD"
                     /locus_tag="IXT20_001869"
     CDS             complement(9628..10914)
                     /gene="sufD"
                     /locus_tag="IXT20_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HAQ4860307.1"
                     /translation="MKEKNWLDYLDAVNDFSLSKGEPDWMRTFRQDALAKADELPLPH
                     IDRVKFHRWSLFDVKETQTISETGTIPAFDAMKDNPVLVQQGSWTIFEQLPVELAEKG
                     VIFTDLFTAMIEYPELVQEYYMKKAVNMNEDQLTALHVAFMNSGIFLYVPKNVVIDEP
                     LESLFIQDGASDEHFFKHVLIVADEHSEFSYLERFQTTKEQVAKSSGNIIVEVIAKAG
                     SKIKYSAVDQLGENITSYMNRRGHILRDASVDWAIGVMNDGHVIADFDSDLAGEGAHA
                     EVKIVAISSGRQIQGIDTRVTNKAPHTIGHILQHGVIREKGTLTFNGIGHILKGAKGA
                     DAQQESRVLMLSDKARGDANPILLIDENEVTAGHAASVGRVDPEEMYYLMSRGLHKEE
                     AERLVIRGFLGSVLTAIPVEQVRKELVEVIEGKLNG"
     gene            complement(10927..11700)
                     /gene="sufC"
                     /locus_tag="IXT20_001870"
     CDS             complement(10927..11700)
                     /gene="sufC"
                     /locus_tag="IXT20_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HAQ4860308.1"
                     /translation="MSVLEIKNLHVSIEEKEILKGVNLTMKTGEIHAIMGPNGTGKST
                     LSAAIMGNPNYEVTEGEILFDGKNVLDLEVDERARLGLFLAMQYPSEIPGITNAEFMR
                     AAINAKRDENDKISVMAFLKKLDEKMALLNMPEEMAERYLNEGFSGGEKKRNEILQLL
                     MLEPTFAILDEIDSGLDIDALKVVAKGVNEMRGDNFGALIITHYQRLLNYITPDVVHI
                     MMEGRVVMTGNADLAKRLEAEGYAGISKELGIDYKEEEA"
     gene            complement(11897..12745)
                     /locus_tag="IXT20_001871"
     CDS             complement(11897..12745)
                     /locus_tag="IXT20_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAQ4860309.1"
                     /translation="MKKKIFGFAAALLLTVGLAACGNNSDSKDSANKADDTTLKVGAS
                     PTPHAEILEHVKPLLKDEGIDLEIVKFDDYVLPNQSLEEGDIDANYFQHIPYLNKEIK
                     EKGYDFVNAGAVHIEPMGLYSKKVKDVSELKDGATIITSNSESDWGRIITILQDAGLV
                     KVKDGVDLETATFDDIEENPKNLKFNHTIDPSLLASTYQNDEGDLVAINANFAYGAGL
                     NPLEDAVLLEKDNSPYVNIIATRKGDEDSDKIKKLIEVLHRKEVQEWITDKWGGSVKP
                     VKADAK"
     gene            complement(12769..13455)
                     /locus_tag="IXT20_001872"
     CDS             complement(12769..13455)
                     /locus_tag="IXT20_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ4860310.1"
                     /translation="MDKSFSETYLDFKQINPETMNQAILDTLYMTLISMVLVTIIGLV
                     LGLILYSIGRKNKPYARIIYGIVSIISNIFRSTPFMILMVLIIPFTKAIVGTMLGAQA
                     AIPALVLSAAPFYARLVEIAFREVSPGVLEAADAMGASYWEIIWKVLIPESIPALISG
                     LTVTTISMIGFTAMAGAIGAGGLGGLAWQEGYQRGNLTVTFVATLIILLIVFIVQGIG
                     DFLTKKTDKR"
     gene            complement(13448..14482)
                     /locus_tag="IXT20_001873"
     CDS             complement(13448..14482)
                     /locus_tag="IXT20_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ4860311.1"
                     /translation="MSLIQLTNVKKTFNGKNGNIQAVNDVSLNVEKGDIYGIVGYSGA
                     GKSTLVRLLNGLELPTSGEVIVNDQDITKLKNKELRTFRKKIGMIFQHFNLLWSRTVL
                     ENIQLPLELAGVPKSKRKERAEELLRLVGLEGRGQAYPSQLSGGQKQRVGIARALAND
                     PEILLCDEATSALDPQTTEEVLDLLLAINKKLNLTIVLITHEMNVIRKICNKVAVMEL
                     GKVVEEGDVLTVFRQPKKEVTKRFVQQDIEPEADSTELLAELIKENPSGRLATLTFSE
                     RNANEPVISQAIRRYNIDVNVVYGKIKQVKEGSFGSLTVLMTGETKELDQAEVFIKEQ
                     GVGIEVIHRG"
     gene            complement(14877..15215)
                     /locus_tag="IXT20_001874"
     CDS             complement(14877..15215)
                     /locus_tag="IXT20_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein H"
                     /protein_id="HAQ4860312.1"
                     /translation="MAGKHLKKKDNLWILFNGKEYCVGLTNEAQEELGDITFANLPKA
                     GQSLKKDEPLIEVEAEKAVNEFASPLTGTVSSVNDKIGEDINILNDSDEMNAWILSFK
                     DVDPKEFDEL"
     gene            complement(15217..15573)
                     /locus_tag="IXT20_001875"
     CDS             complement(15217..15573)
                     /locus_tag="IXT20_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HAQ4860313.1"
                     /translation="MYTFYWYPKCSTCKKAKAWLDQHGVSYQTVDMIKEVPKASTIET
                     WLETSQVPIRRLFNTSGMKYRELGLKDQVGEFTASKASQVLSTDGMLIKRPLILREDQ
                     LVAIGFNEKIYEGELI"
     gene            complement(15579..16766)
                     /locus_tag="IXT20_001876"
     CDS             complement(15579..16766)
                     /locus_tag="IXT20_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HAQ4860314.1"
                     /translation="MEKKLKASNDNRIDYGVILPVFLLCLIGLLSLYVALSHDPNNPA
                     VLKGVGMQVVWYLVGAAAIVVIMHLNSKWIWKLTPYFYGLGLVVMGLLLKFYDQNLAA
                     STGSKNWFRFGAFTFQPAELMKIAYILMMALIVTKHNTQVKERTMKSDFWLIGKLLIV
                     TIPVLALIMAQDDFGTMLVFLAIFGGIFLMSGISWRIIAPVVILAVVVGAGTIFLVTT
                     EGGRDLLYKVGFKSYQFARIDSWLDPFHDTSGMSYQPAQGLLAIGTGGLFGKGFNVSN
                     IYVPVRESDMIFTVVGENFGFIGGAFVIFLYFILIYRMIRVCFDTNNEFYAYIASGLI
                     MMLLFHVFENIGANIGLLPLTGIPLPFISQGGSSILGNMIGIGLILSMRYQNEAPARS
                     SRR"
     gene            complement(16957..17802)
                     /locus_tag="IXT20_001877"
     CDS             complement(16957..17802)
                     /locus_tag="IXT20_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAQ4860315.1"
                     /translation="MFKLLTTFRAVYETRNFSRAAEVLFLSQPAVSNQIKQLERELNI
                     DLFQRNGRKEMLPTKQADLLYERTLVLLEEWEETQQKLLNEQNEEEVCRIVASHTFAV
                     YPLPQLMKHLIKRFPKVHFEIEIANSHESLEQVAKHESDFGFIEKPLETSGVQRYSLM
                     EDQLVIAGDPKSKLWLVREANSGVFHYTQRYFEEMNIQDKKMIVKSNDVIVALLKEGI
                     GRSILSKRSVPENVPMTPLNFHRYFYFIQRTNLVSDQLLEIAEEILRYNESTETSHSE
                     ENLSW"
     gene            18036..19001
                     /locus_tag="IXT20_001878"
     CDS             18036..19001
                     /locus_tag="IXT20_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HAQ4860316.1"
                     /translation="MKNFLKIIPIPICGLILGLASLGNLFKDYHHVALGNLTGGISMI
                     LMIFIFVKLFILFEHTKQTLHDPIIASVSPTFTMSLMVICTYFVSYESIAPIVKFIWL
                     CAVIFQVILVFYFNYHHVIKADLSIEAIYPSWFIIYVGFGVITVTAGNFFPLIGKIFF
                     WISLICYAFLLPIIIKRVFFVRNMAHPTRPLITIVAAPGSLCLTGYLKNFAEPNFYLV
                     VALFALSQVLYFVVLSMLPRLFKLRFYPSYAAFTFPLVISATAIFTTVHYFNSLGIYS
                     SVLDFLKVAECLFASVVVFYVLAHYVRFLIKEHSKHGKFVSKKIA"
BASE COUNT         5471 a         4040 c         3291 g         6472 t
ORIGIN      
        1 gcatgacaat cccccctttt atttttttaa tagattatcg aaaagtggat taggatcatt
       61 aagtgtatct ctgaataact cattgtcttc ataagtgaat cttcgtcttg attttgatcc
      121 cttatcattt tcacggattt cactatcaat aatcactgtt tcatgatttg aatcaaattt
      181 tttcgctgac tttctgtctt caacaatatt gattttttgt gctttttctt tcatttctcc
      241 actatctttt tgaacgttct cccgaacatc tactgttttg aatccttgac tacttgtaga
      301 actttgtaca ttttctcgta cgtctactgt tcgatttcca ctttgactac ttgtagtatt
      361 ttgcaagttt tctcgaacat ctactgttct cggttgactt gtcccactac ttagtacatt
      421 ttctcgtaca tttactgttc ggtttccact ttgactactt gtagtattct gcaagttttc
      481 tcgaacatct accgttctcg attgatttga cgcactactt tgtacatttt ctcgtacatc
      541 tactattcga tttccacttt gactacttgt agtattctgt aagttttctc gaacatctac
      601 tgagcgtcta tcttgactac ttgtagaatt ttgtagattt tctttgacca atttttctaa
      661 tccggtattt gaatttttag cttcttgaat tctttctttg gtcaacgctt ctttttcgtc
      721 catattagcc cctgtacgcc cttctacacg tttcctcaac atttcctcac gattcattat
      781 ttcaggcgtg tattttgctt cctgtcgctt ctgattaatt aattcttcag cagacttagc
      841 ttgaccgtgc atagcatttt tacgttcttc aatatcttgt ttaacttttt gctgaattgc
      901 acttgcatga ttagtatttg attcagcatt atttttcatt gcttcacgca tacgttcaga
      961 actagacata attctatcag cgttttcaac tttagcgcca tcttttctat ccgaaagacg
     1021 cgttggcata gaatcagcat attttccagt ttcatcttct ttatttttag ctactatagg
     1081 tttgctattt ttattaatag cggacacggt accatcatct aaaccttctt tgaatttatt
     1141 cttagtatca ttagctatat cacgcatact cttaaatggt tttgttgctc tttcactagc
     1201 cattgtagct ccatccgaag ctaagcctga taatccacgt tcacgagcat acccaagcgt
     1261 tttccccgtt ttattagctt tttgagaaag tgattgacca aagttgcgtt caggttttcg
     1321 ttcttcccct tccgcttgat cgtttccacc actatttttc ccacctttag atcggttcat
     1381 accacctgtt cctttacgaa acagcatagc tcccatacca aatgctgaag caagctggcc
     1441 atagccttct tttaaaccaa tatccacgcc aaagtaacgc atgactgttc cactaccctt
     1501 tattagaacg aacacggcac agatataagc aattgttttt ataaaaatat tcgttgagat
     1561 accttcacca gcgtttagat aattaacaaa cattgcaaac atagacaatc cgaatccctg
     1621 aaaaccgaca gtcaaataac actgtagaat atctgacaaa acttgttttg atcgttgtcc
     1681 tgtttctaag tcagtagcaa ataccaaaac tccaagtacg cgtttaaatg ctaactcgag
     1741 tattgctgta attataatga aagcactaaa aacatacgct actgctaaag caatcaatcc
     1801 aaatgaaatt ccccacaagt tcgcttgata gcgataatat cctgatttga taccatcaac
     1861 aaaggttgac aaaaaattat ctgaaaattt agtagcaaca aattcattat cagaattttc
     1921 aactaattca taacgcagat tatcagctat tgggttctct ttttccattt tgtcgagtac
     1981 attatctgtc aatacttgcg taagatccgt tttccaaaaa ttttcttttg ttagattgtt
     2041 ccggtcattc gttttatcga tcatggaata ttgatccgtt ttattgatat aagctaaatc
     2101 agtaacccct tgttcaatca gcgaccacgc cacgccttcg tctgaatctg taatcgtagt
     2161 tgaatcatta taaaaggttt tagaaaactc tattccgctg tgaatcaaag ttggcattaa
     2221 aagaatcaat gaaattgaca ttacaacatt cattccaaca gattttagat caaccgttcc
     2281 tttacctata atcattttat aaccaacaat cactagtgaa agtatcatta agccaacaac
     2341 aatggtactt aaaactgatt gatagacttt gttcaatcca gcactttcca caaagctaaa
     2401 caaatctaaa acctcgggaa tcatattttg aatgccatcg acaacagcga acattgattt
     2461 tataacagac cagcctatcc acataccgaa ctgactgtaa aaatttccgt tatatttttt
     2521 aagataattc tgataaagtg gattagttaa aaactcagtt tggctttttg tatcatcaaa
     2581 ccagttgaaa atactgcctt ctgcaacgtt actcgtaaaa ataatcatag ctatgagaat
     2641 aagaaaacta atcaacaacc aatttatttt tctattggca atcttttttc caataatttt
     2701 cattgttacc tccctcagcc cagtttctga ggctctcaga gagcttttga gcaatttttt
     2761 ggtcaatgat gatatgagtt acataactat cattcactgc tcttaaatcg agaacaatag
     2821 tgttgaagca gatacactcg attaattcaa gtgtatctgc gttttggtca agacagcaga
     2881 tttgatctat tagacgattt ccaatcatat taagagtcct ttcttgttta tttacttaga
     2941 gcatctatga tatacattct gttatcttgt tcttctaaag caatttcttt ccagtgattt
     3001 cgagataccg tgtttacata cttcatttca ttcaattctt tacccaagtt taatttctcg
     3061 ttaatcgtat taaataatct gattatcatc ttatttgttt gttcatagta atgtggccca
     3121 tcgattcgaa ttttgaactc tctttctgtt gaatttttca tatctactat taattgaacg
     3181 aatttttctc tttgactttt agtaaatctg tcacctagtt tatgaatcag ctgtattcct
     3241 ttctcgttaa ttatctcttc attgataata gtatcaactt gattaatctt atctatacta
     3301 ttcttaacta aactagattt atctgaacta atctgatctt gacttgactg aacctccaaa
     3361 tggttgatgc taggtatacc aactggatac catttttcca ttaaatcgac tcttagcggt
     3421 tctacttggc cagatttcaa aatcacatca agttttattg gctttaattt tgagaaaaat
     3481 tctggtttta agttcaataa cttgaagcga taaatagatg gtttatacct atctattcgt
     3541 atattttggt tttgtaacca atgcggtagg aatgctaaca aatcttcatt taaaaaaaat
     3601 acaaaacctt ttgtaaaaag tatctttaca tcatcgtcag gagtcccgag cattttcacg
     3661 tatggatatg cttcaacaat tccgtcatcg tctgcatttg tgttcagata tacatataat
     3721 aattttgctg aatctgacat cttcataaaa cttgcattat ttaaaaatgt tttggacagc
     3781 attcgttttt ctgccatgga tttcacactt ctttctattt gaatggtaaa ataaattcaa
     3841 ataaagtttt atcagaactt tattgttggg tgtttccctt cgtctacaaa ctatcgggag
     3901 cactctttta tatatttttt tcaattcttt tcaagtctaa atttgttatt tcattttctc
     3961 tcaaaacttt cacctctttt cattcgagca aacataaact cattaactgc ctttttcgaa
     4021 taaaattttc ttgcccctaa ttcaacaacg ggcaatccct catttctcac cgcatttttt
     4081 aaaaagtttt cgctcacatt taagtattct gccatttctt tttgatttag atattctcta
     4141 gtcatacccg cattttcttg agccttctca atcgcttttg tagcaatttg ataaaactgc
     4201 tttagaaaca tttcctcctg ttcttctgaa atcattaatt ttattgcttc cacaaaaacc
     4261 tctccttttt aattaattat tctataactc ctctaccttt ataagtacaa ataaatacaa
     4321 attgcgtaaa aaaatgtatt tatttgtatt tataagaata aagtatcata ctccagataa
     4381 aaagtaaata cttttaaata cttttatttg ataaatatga tatactatct ttaaaaataa
     4441 aaaggagaaa aaagatgaag ccaaatcctt cagaagcagg aaaacgaatc aaacaattac
     4501 gcctttcatg tggttttact atggaagaac ttggaaggaa aatagataat tcaccacgtg
     4561 cgaccatatc caactgggaa cgtgggacca atttaccaaa tccacaaaag ctgaaattat
     4621 tatcaactat cactaattca acaattgact ggattaaatg gggaacttta gaagaatata
     4681 taacttcata ccttatcgat atagggtatg agctatatat taaggacttt ccagaaatac
     4741 ctcacaaagt ctttaaagac attcaagaaa gatatagtga tacgttttcg ctagataaag
     4801 actatgagtt attgaatcct ataataaaaa atatatttac aaaatattac tcaaaagatt
     4861 ttgaaattta tctaaatgag atacttgcta ataatatcaa tcctctgatt gaagagtacg
     4921 ctaaccaaag acactctatc tctaaagagc tatttttcaa aagagccaac agtaaacttc
     4981 aagacaagat aagaaatggt gatttcaaat acggtgaaaa agaaagaatg attgattctt
     5041 taaaacagct attagacgaa atggacttag cctataaaga tatttcaaag tattcatctg
     5101 tcgaggattt cttttataaa attactaaaa accaatttga aacagaaaaa tttatttcag
     5161 atcttagtaa aaaatacaat ttcccatatg agaaaaatag tataatcgca aaatttttaa
     5221 ctgataatca cgataaattt aaaaataatt gattcttcaa ataacttctc tacctgcaat
     5281 aatgaagtaa ggagagaatc aaatggcaac attttttagt tataaattac aaaatggcga
     5341 aaaatattgg ggatttgaaa cttatttagg gaaagatccc aagactggaa aagatatacg
     5401 tacaaagcgt agaagattca aatcaaaagc tgaggcgcaa acagcactta atagactact
     5461 tgtagacttt aaagaaaaac aagagtttaa aatcaatgtt acaacttttc aagagttgta
     5521 tgaattatgg attgataact acagaacaag agtaaaaccg tcttctgttg ccgttgctca
     5581 acgtttttgc aaaaaccata tattacctgc ttttggggaa ttacgattgg ataaaataag
     5641 tgttagctat tgccaaaaac aggtgaatga atggcacaaa acattaaaac aatatggttt
     5701 cttgagaaaa acaactgctc aaatttttaa atttggagta gcaatggaag tctgtaagga
     5761 taatcctatg tctaaaacat tgcttccaag aaaaattgaa gaagaacaac ccttgaagtt
     5821 ttacacgaaa gatcaattac aactgttttt acagaatact aaagaaaata attcagttaa
     5881 aatgtacact tttttccgtt tattagctta tacaggaatg cgtaaaagcg aggcattggc
     5941 gcttcaatgg gaagatatag actatttcaa caaaacaata acaattggta aaactatcgc
     6001 tcaagacgag tttaatcaag tagttttaca agtacctaaa actaaaaatt catcaagaac
     6061 gattcagctt gatgatttta cacttaaaca actgcgtatt tggcaacaag aacaaatgaa
     6121 aataatgatc ttatatggct ataacacgaa ttcaccaaaa cagtttcttt tcactacaaa
     6181 cacaaacaag ttgtactacc cccaagtagt aaacgattgg ttagactgga tttacaaaaa
     6241 gacaccgatg gagcctcaaa ttactccaca tggatttagg catacccact gtagcttatt
     6301 gtttgaaagt ggtgcgtcga taaaagaagt acaggaacga ttaggtcata aagatattaa
     6361 aactaccatg aatatctatg ctcatgtcac accacaatct gttaagaaaa ctggtgaccg
     6421 atttgcaaaa tttatgggcg aataattcca aacgtagcca aaaacgtagc caaccattaa
     6481 aatacaaata aaaaaacgct cgaaacgctt taatatcaac gtttcgagca atgaaatagt
     6541 tgttaaccca cagacccttc catctcataa ctgatcaatc ggttgagttc gactgcatat
     6601 tccattggaa gttctttggt gaatggttca acaaagccca tgacgatcat ttcagttgct
     6661 tcagattcgg tcaggcctcg gctcatcaga tagtagagtt gttcttctga gatttttgag
     6721 actttggctt cgtgttctaa agagacttgg ctgttgtgga tctcattgaa tgggatcgta
     6781 tcggatttcg accattcgtc catgatgatc gtgtcacatt cgatatgaga gatcgaaccc
     6841 gcactgtttt tggcaaaggt tacttgtcct cgatagttta cttctccgcc gtcttttgaa
     6901 attgatttag agacgataga gctagatgta tttggtgcat tatggatcat ttttgcaccc
     6961 gtatcttgga tttggtttgc tcctgcaaag gcgatggaga gcatggtgcc tcgtgcacct
     7021 tttccatcta agtaaacact tgggtatttc atcgttgttt ttgcacctaa gtttccgtcg
     7081 atccattcaa cggttgcgcc ttcgtatgct tttgctcgtt tggttactaa gttgtacaca
     7141 ttgtctgacc agttttggat cgtggtgtaa cggcaatacg catcttttct tgtaaagatt
     7201 tctacgatgg cagcatgcag actattactt gaataagtag gtgccgtaca accttcgaca
     7261 tagtggatgc ttgctccttc gtcaacaatg atcagcgtac gttcgaattg ccccgtgttt
     7321 tccgcattga tccggaagta cgtttgtaat ggaacatcga ctttgacgcc ttttggcacg
     7381 taaataaagg tgccacctga ccatacggct gaattcagcg ctgctaattt gttgtcggtt
     7441 ggcggtacaa gttttgaaaa gtactctttg aataattcag ggtattcttt caaagcagag
     7501 tcagtgtcag taaagacgat tcctaacttt tcgaattcat ctttcatatt atggtacacg
     7561 acttctgatt catattgtgc ggaagcaccg gctagataag ctcgttcagc ttctggtata
     7621 ccaatctttt caaatgtatc tttgattttt tctggtacgt cttcccagtc acgagcgggt
     7681 ttgtcacttg gtttttggta atatttgatg gcatcgaaat cgatgtctga aaggtcgggc
     7741 ccccatgttt gcattggcat tttattgaat gtttctagag atttcagacg gaattcaagc
     7801 atccattctg gttcgccttt gattcttgac atctcacgga tcacttcttc tgtcaagcct
     7861 tctcctgtac tgaaaaccgg ttcgacatca tcgtgaaacc cgaatttata ttcttctaat
     7921 tctggtacgc ccatgttctc actcctcttc cttgtggtaa tgatggaaag ctgcggtacc
     7981 atcgttttcc attgcttttc ctagtgcttt ccaggcaagt gtcgcacatt tgatccgagc
     8041 gggaaattta gctacaccac caagcatagc agcatcgcct aattggtcgg gatctttgac
     8101 ttcttttcct tggacaagct gtagaaagtc tacagttaaa tgttctgctt cttctattga
     8161 ttttccaatg acggcatctg tcatcatcga ggcacttgct gtactgatgg aacagccgct
     8221 gccgtcaaag gcgatatttt tgatgattcc atcttttaca tcaacttgca attcgatcac
     8281 atctccacat gtggggttgt tcaattcgat cttgcttgtc gagtcagtca agcttccacg
     8341 atgatgtgga tgggaagaat gatccaagat cacttgacgg tataaattgt ctaatttaga
     8401 tagtcccatg tttgaaaaac tcctttgttg ccatgattgc ttcaactaaa cggtcggcgt
     8461 cttgcgttgt gttgtagaaa taaaagctgg cgcgagcagt cgctgatact tcaaggtact
     8521 tcaatagcgg ttgcgcacaa tgatgacctg ctctaacggc taccccttcc atgtctaaag
     8581 ctgtcgcgac atcatgggga tgtaacccat ccaaattgaa cgcaatgact cctgtccgaa
     8641 aattcgggtc ttgcggacca taaacagtca atccatcgat tgcttgtaat tttggcaaca
     8701 cataagcggc tagttcttct tcatatcgat ggatattgtc catgccgagt tcttgcaaat
     8761 aatcgattgc atgacctaat gcgatagcgc cagcgatatt tggtgtccct gcttcgaatt
     8821 tccaaggtag ttctttccat gtactgtcgt ataattcgac aaaatcgatc atttcgccac
     8881 caaattcaac aggttccatt tcttctaacc attttcgttt accatataaa acaccgatac
     8941 ctgttggtcc gcacattttg tgtccgctga aagcaaagaa atcacagtct agtgcttgaa
     9001 catcaatggt catgtgcggg gcagattgag caccatctac tacgatgacc ccgccgtttt
     9061 catggacaag ttcagctaat tcttgaacag gattgatgac acctaagaca ttggatacat
     9121 gagcaatgga gacgattttg gttttgtccg taatcttttt cgctgcatcc tccatatcca
     9181 ggaagccttc ttctgttagt tcgatatatt tcaagacagc acctgttcgt tttgccaact
     9241 gctgccacgg gataacatta gaatgatgtt ccatgtaaga gagcactatc tcgtctcctg
     9301 ctttgatata gcggtcgccg aagcttctgg ctacccaatt caagcttgtc gtcgtaccac
     9361 gagtaaacag cacttctgct gtttcttttg catggatgaa cgcccgtact ttttctcttg
     9421 cttgttcata atcatgggtc gcgcgttctg ctaacgtatg gacaccgcga tgaacattgg
     9481 cgttatcgtg ttcgtaatag tgcgctaatg cctggagtac ttgttttggc ttttgggtcg
     9541 tcgctgcatt atccagataa actaaaggtt catcattgac gtcttgaaat aagataggaa
     9601 aatcattgcg tagcttttta gcatcgatca tccattcaac ttcccttcga ttacttcgac
     9661 taattctttc cgaacttgtt ctacagggat tgctgttaag acagaaccga ggaatcctcg
     9721 aatcactaag cgctctgctt cttctttatg caatcctcga ctcatcagat agtacatttc
     9781 ttctggatca actcgtccta cactggcagc atgtccagca gttacttcgt tttcatcgat
     9841 caaaagaata ggattcgcat caccacgtgc tttatcagaa agcatcaaga cacgactttc
     9901 ttgttgggca tctgcacctt ttgccccttt caagatgtgg ccgataccat taaaggtcaa
     9961 tgttcctttt tctcggatca ctccgtgttg caaaatatgc ccgatcgtat gaggggcttt
    10021 attggttact cgcgtatcga ttccttggat ttgtctgccc gagctgattg ccacgatttt
    10081 gacttctgcg tgtgcacctt ctcctgctaa atctgagtca aagtcagcga tcacatgacc
    10141 gtcattcatg acaccaattg cccagtcgac agaagcatct cgtaaaatgt gcccacggcg
    10201 attcatataa gaagtaatat tttctcctaa ttgatctacg gctgaatatt tgatttttga
    10261 accagcttta gcgatgactt ccacgatgat attacctgat gattttgcta cttgttcttt
    10321 ggttgtctgg aaacgttcca gataagaaaa ttcactatgt tcatctgcca cgatcaacac
    10381 atgcttgaag aagtgctcat cggatgcgcc atcttgaatg aataaggact cgagtggttc
    10441 atcgatcacg acatttttag gcacatacag gaaaataccg ctgttcataa aagctacatg
    10501 gagtgcagtc aattggtctt cgttcatgtt tactgctttt ttcatataat attcttggac
    10561 tagttctgga tattcgatca ttgcagtgaa caagtcggta aaaatgactc ctttttctgc
    10621 taattccaca ggcagttgtt caaaaatcgt ccatgaacct tgttggacaa gcacaggatt
    10681 gtctttcatt gcatcaaatg ccggaatcgt gcctgtttca ctgatggttt gtgtttcttt
    10741 cacatcaaat aacgaccagc gatggaattt cacccgatcg atatgcggca atggcagttc
    10801 atctgctttc gctagtgcgt cttgacggaa ggttctcatc caatcaggtt cgcctttact
    10861 taaagaaaaa tcatttaccg cgtcaagata atcgagccag tttttttctt tcattcttgc
    10921 tcccccttac gcttcttctt ctttatagtc gattcctaat tctttactga taccagcgta
    10981 gccttctgct tccagacgtt tagctaggtc agcattccct gtcatgacta ctcggccttc
    11041 catcatgatg tgaacgacat caggtgtgat atagttcaat aatcgttgat agtgggtgat
    11101 gatcaatgca ccgaaattgt ctccgcgcat ttcatttact cctttagcaa cgactttcaa
    11161 agcatcgata tccagacctg aatcgatttc gtctaaaatt gcaaatgttg gttctaacat
    11221 caacaattgc aggatctcat tccgtttctt ttctccgcca gaaaaacctt cgttcagata
    11281 acgttcagcc atttcttctg gcatatttag taaagccatt ttttcatcta actttttcaa
    11341 aaatgccata acagaaattt tgtcgttttc atcacgtttc gcattgatcg ctgcacgcat
    11401 gaattccgca ttcgtgatac ctggtatctc acttggatat tgcatagcta aaaataagcc
    11461 aagtcgcgca cgttcatcta cttccaagtc taaaacattt ttcccgtcaa acaaaatttc
    11521 gccttctgtt acttcatagt ttggattgcc catgatagcg gcagataagg tagattttcc
    11581 tgtgccgtta ggtcccatga tggcatggat ttctccggtt ttcattgtta ggtttacgcc
    11641 tttaaggatt tctttttcct cgatcgacac atgtagattt tttatttcta agacagacat
    11701 gcattatacc cccaaattct ctttcgttct cactcatttt agactaattg agaatgaagc
    11761 gcaatgacct tccttactaa aatcaaatat cttttattta ttattaaaca attttgattt
    11821 ctaaattaga ataggtataa aaaagatgag aaataagcca ttacagactt atttctcatc
    11881 ttttttgttc cataccttat tttgcatcag cctttactgg tttaacagag ccgccccatt
    11941 tgtctgtgat ccattcttgg acttctttgc ggtgaagcac ttcaatcagt tttttgattt
    12001 tgtcactgtc ttcatcacct ttacgagtcg caataatatt cacgtaagga gagttgtcct
    12061 tttcaagcaa tacggcatct tctaatggat tcaaccctgc gccgtaagcg aagtttgcgt
    12121 tgattgcaac taggtcacct tcatcatttt ggtacgtaga agctaaaagt gatgggtcga
    12181 tcgtatggtt gaacttcaag ttcttagggt tttcttcaat atcatcgaat gttgctgttt
    12241 ccagatctac tccatctttg actttgacta agccagcatc ttgtagaatt gtgatgatgc
    12301 gtccccagtc tgattcagaa tttgaagtaa tgattgttgc accatctttt aattcggaaa
    12361 catctttgac ttttttagag tacaagccca ttggttcgat gtggacagca ccggcattga
    12421 caaagtcata tcctttttct ttgatttctt tgttcaaata aggaatatgc tggaagtaat
    12481 tggcatcaat gtcgccttct tccaaagatt ggttcggtaa tacatagtca tcgaatttga
    12541 cgatttcaag atcgattcct tcatctttca ataatggttt cacatgttcc aaaatctctg
    12601 cgtgcggtgt cggtgaagcg ccgactttca atgtcgtgtc gtctgcttta tttgctgaat
    12661 ctttgctgtc gctgttgtta ccgcatgctg ctaatcctac agtcagcaaa agcgctgctg
    12721 caaatccaaa aattttcttt ttcatatcct acatccccct atatcctatt atcttttgtc
    12781 tgttttctta gttaagaaat caccgattcc ttgaacgata aatacgatta gtaaaataat
    12841 taaagtcgca acgaatgtca ctgtcagatt accacgctgg tagccttcct gccaagcaag
    12901 acctccaagt ccacctgcgc cgatcgcgcc ggccatcgca gtaaatccga tcatcgagat
    12961 cgttgtaact gttaatccag aaatcaatgc gggaatactt tcaggtatca agaccttcca
    13021 gatgatttcc cagtaacttg ctcccattgc atctgctgct tccagtacac cagggctgac
    13081 ttcacgaaaa gcaatctcta ctagacgggc ataaaatggt gctgcggata aaacaagagc
    13141 tggaatggct gcttgcgcac ctagcatagt gcctacgatt gctttagtaa atggaatgat
    13201 caaaaccatc aggatcataa atggcgttga ccggaaaata ttactgatga tcgatacgat
    13261 tccataaatg attcgagcat acggtttgtt tttgcgtcca atagagtaaa ggatcaatcc
    13321 caaaactaat ccgatgatcg tcactaagac cattgagatt aatgtcatat atagtgtatc
    13381 aaggatagcc tgattcatcg tttccgggtt gatttgttta aaatctagat atgtttctga
    13441 aaagctttta tccacgatgg atcacctcaa ttcctactcc ttgttctttg atgaacactt
    13501 ctgcttgatc taattctttg gtttcaccag tcatcaagac agtcaatgaa ccaaaggaac
    13561 cttctttcac ttgtttgatc tttccataca caacgttgac atcgatgtta tagcgtcgaa
    13621 tcgcttggga aatgactggc tcgttggcat ttctttcgct aaaggtcaaa gttgccaaac
    13681 ggccgcttgg attttctttg atgagttcag ctaatagttc tgtcgaatca gcttctggtt
    13741 cgatgtcttg ttgtacaaag cgtttggtta cttctttttt cggttgtctg aagacagtca
    13801 atacgtctcc ttcttctacg actttcccca actccatcac agcgactttg ttgcagattt
    13861 tgcggatcac attcatttca tgtgtgatca agacgatggt caaattcaat ttcttattga
    13921 tcgctaacaa taagtctaat acttcttctg ttgtttgcgg gtccaatgcg ctagtagcct
    13981 catcacaaag caggatctca ggatcatttg ccaatgctcg tgcgataccg acgcgttgtt
    14041 tctgcccgcc ggaaagttgt gaaggatatg cttgtccgcg tccttctaac ccgactaaac
    14101 gcaacagttc ttctgcccgt tctttacgtt tgctctttgg tacgccagct aattccagcg
    14161 gcagctggat attttcaagt acagttcttg accataacag gttgaaatgc tggaagatca
    14221 taccgatttt ctttcggaat gtacgtaatt ctttgttttt taattttgta atatcttgat
    14281 cgtttacgat cacttcgccg ctcgtgggaa gctcaagtcc attcaggaga cggactaatg
    14341 tactttttcc tgcgccagaa tagccgacga ttccataaat atcccctttt tcaacgttta
    14401 gtgaaacgtc attgacggct tggatgtttc cgtttttccc attgaatgtc tttttgacat
    14461 tcgttagttg gattaatgac atgccattca catctttctc ttatgatttt tcaaacaaaa
    14521 aagccttcgc cctcttgcct taagcaaaag gacgaaagct tgaacttccg tgttaccacc
    14581 ttgatttacc gttacttcgc aataacagcc tcttccagta cttggactta gcttctttcc
    14641 gtatactgtc gcgctgtaac gggcactccc gtagtaagtt tgcttacgct cgcttactct
    14701 gctctaagac catcttccat ctcattttcg ttacccattt tcagcaaaca gggctctctt
    14761 caaacgtccg tgacatgtac tcttcttttc aaagctttct cgtttcatat aggattgctt
    14821 atgattatag caactggaag aatcagtgtc aacttttttc aagtgatttt ctgattttat
    14881 aattcgtcaa attcttttgg atcaacatct ttgaaactta agatccacgc attcatttca
    14941 tctgaatcat tcaagatatt gatatcttca ccgattttat cattaacaga agaaactgta
    15001 cccgttagcg ggctggcaaa ttcattgacc gctttttccg cttcaacttc gatcagaggt
    15061 tcatcttttt ttagcgattg tcctgctttt ggcagattgg caaacgtgat gtctccaagt
    15121 tcttcttgcg cttcatttgt caaaccaaca cagtattctt ttccattaaa taagatccaa
    15181 aggttgtcct ttttcttcaa atgcttgcct gccattttat atcagttctc cttcataaat
    15241 tttttcattg aatccaattg caacaagctg gtcctctcgt aagatcagtg gccgcttgat
    15301 cagcatacca tctgtcgata agacttggct cgcttttgaa gccgtaaact cgcctacttg
    15361 atctttcagt ccaagttccc gatatttcat cccgcttgtg ttgaacaggc gacggattgg
    15421 tacttgactc gtttctagcc aagtctcaat cgttgaggcc ttcggtactt ctttgatcat
    15481 atcaacagtt tgataactaa cgccatgctg gtctaaccac gctttggctt ttttgcatgt
    15541 tgaacatttc gggtaccagt aaaaggtgta caattttctt acctccttga agatcttgca
    15601 ggtgcttcat tttggtatct catcgataat atcaacccga taccgatcat gttccctaaa
    15661 atcgaagatc ctccctgact aatgaaaggc aatgggatcc ctgttaaagg aagtaatccg
    15721 atatttgctc caatattctc aaagacatgg aacagcagca tcatgatcaa accgcttgcg
    15781 atataggcgt aaaattcatt attcgtatcg aaacacaccc gaatcatacg atagattaaa
    15841 ataaaataca agaaaatgac aaatgcacca ccgataaagc cgaagttttc accgacaacg
    15901 gtaaaaatca tatcggactc acgaacaggc acataaatat tgcttacgtt gaatccttta
    15961 ccaaacaagc caccggtacc aattgccaaa agtccttggg ccggctgata actcattcct
    16021 gacgtatcat ggaaagggtc cagccatgaa tcaatacgtg caaactgata agatttgaag
    16081 ccaactttgt aaagcaagtc tcttccgcct tcagtcgtaa ccaagaagat cgttcctgct
    16141 ccaacgacta ctgccaagat tactacaggt gcgatgatgc gccaagagat tccagacatc
    16201 aagaaaatcc cgccaaagat cgcaagaaag acaagcatcg ttccaaagtc atcttgggcc
    16261 atgatcaatg ctaaaaccgg tattgtcacg atcaagagct tcccaatcag ccaaaagtcc
    16321 gatttcatcg tccgttcttt tacttgcgta ttatgtttag ttacgatcag tgccatcatc
    16381 aagatgtacg caatcttcat caactctgct ggctggaaag taaacgcacc gaatcgaaac
    16441 cagtttttag accctgttga agcagctaaa ttctgatcat agaatttgag caagagtccc
    16501 attaccacta accccaaacc ataaaagtaa ggagtcagtt tccagatcca tttggaattc
    16561 aaatgcatga tgacaacgat tgctgccgct ccaactaaat accagacaac ttgcatccct
    16621 acgcctttca agactgctgg attgttcgga tcatggctaa gtgctacata taatgagagc
    16681 agaccaatca aacataacag aaagactggt aaaatcacgc cataatcaat acgattatcg
    16741 ttcgaagctt ttaatttttt ttccatgttt catccctttt cttaaaaacc atactgctga
    16801 ttattatagc ggtccagtta gataatggaa agtctttcca tagacatttg ggaaaaatta
    16861 actttccgtt tgtgcagttg cttgagcaaa tgcagtacaa aagcctactg cttatcttta
    16921 gtgtatcata gaactttttt tgatacatgc aattatctac caactgagat tctcctcgct
    16981 gtgagaagtt tctgtgcttt cattgtatcg aaggatctct tctgcaatct ctagcagctg
    17041 atcagaaacc agattggttc gttggatgaa ataaaaataa cgatggaaat tcaacggagt
    17101 cattggcaca ttttctggaa cagagcgctt agacaaaatc gagcgaccga ttccttcctt
    17161 cagcaacgca acaattacat cgttactttt cacgatcatc ttcttatctt ggatattcat
    17221 ctcttcaaaa taacgctgcg tgtaatgaaa aacaccagag tttgcttctc ttacaagcca
    17281 caatttgctt tttgggtccc cagcgatgac tagctggtct tccatcaaag agtatctttg
    17341 tacgccggaa gtctctaatg gcttttcgat aaaaccaaaa tcggattcat gtttcgctac
    17401 ttgttctaat gattcatgag aattcgcgat ttcgatctca aaatgcactt taggaaacct
    17461 tttgatcaga tgtttcatca actgaggcaa aggatagaca gcaaaggtgt gggaagcaac
    17521 gattcgacat acttcttctt cattttgttc attgagcaat ttttgctgag tttcttccca
    17581 ttcttctaac aaaaccagtg tccgttcata taataagtcg gcttgcttag ttggaagcat
    17641 ctcttttctt ccgttacgtt gaaaaagatc gatattcaat tcccgctcca actgtttgat
    17701 ttgattcgag actgctggtt gagataaaaa cagtacttct gctgctctgg aaaaatttct
    17761 tgtttcatat actgctcgaa aagtcgttaa taacttaaac atggcaccac ttccataact
    17821 ttttatgata attactataa aaactatttg atttaaatat acaaaaaacc atctataata
    17881 gaaatgtaca acaaaacagt tgctgatcca actagtaact tagatgaaga caatcagctg
    17941 tctgcttgct cgtaatcata cattcctatt cattttacaa tgtattcgtt catcttggta
    18001 ctaaaatcta ttaaataaaa aggagaatat tatatatgaa gaactttttg aaaattattc
    18061 cgatcccgat ctgcgggttg atccttgggt tagcttccct tggaaatctg tttaaagact
    18121 atcaccatgt tgctttaggc aatctaactg gcggtatctc tatgattctt atgatattca
    18181 tttttgttaa attgtttatc ttattcgaac acacgaaaca aacgttgcat gatccaatca
    18241 tcgcctccgt ttcaccaact tttacgatgt cactcatggt tatttgtact tattttgttt
    18301 catatgaaag tattgcacca attgtcaaat tcatttggct gtgcgctgtt atcttccaag
    18361 tgatccttgt cttttatttc aattatcacc atgttatcaa agctgatctt tcaattgaag
    18421 caatttatcc aagctggttt atcatttatg ttggatttgg ggtcatcact gtaactgccg
    18481 ggaacttctt cccgcttatt ggaaaaattt tcttttggat ttcattgatc tgctatgcgt
    18541 tcttattgcc gatcatcatc aaacgtgtct ttttcgtaag aaatatggct catccgacac
    18601 gtccgctgat tacgatcgtt gcagcacctg gttctctttg cctaacaggt tatcttaaaa
    18661 actttgctga acctaatttt tatcttgtcg tagcattgtt cgctttatct caagtattgt
    18721 atttcgttgt gctgtctatg cttccacgtt tgttcaagct tcgtttttat ccaagctatg
    18781 cagcctttac attcccgctt gttatctctg caacagctat atttactact gttcattact
    18841 tcaacagttt aggtatctat agtagcgtat tggatttctt aaaagttgcg gaatgtctgt
    18901 ttgcaagtgt agttgtcttc tacgttcttg ctcattacgt acgtttctta attaaagaac
    18961 atagcaaaca tggaaaattt gtttctaaaa aaatcgctta ataatcgatt caataggttg
    19021 tgaaaaagct gttctgcatc aagtaataag aacggccaaa caaaaacagc tgttcatgtt
    19081 tttgaattgg ttgggcttat taccgcagat gcaagctttt gaacaccgtt taccaagagg
    19141 tcgtaaaaga agtggccagc ttcaagaaat aagaaggaat ttacgaaaat tgcttctcaa
    19201 atttttgtga aatttcggct tatttccgaa aaagctactt ctggaacacc gtttaccaag
    19261 aggtcgtgaa aaag
//
LOCUS       DACGLE010000050        18968 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0534
            SAMEA3304449-rid9560453.denovo.054, whole genome shotgun sequence.
ACCESSION   DACGLE010000050 DACGLE010000000
VERSION     DACGLE010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304449
            Sequence Read Archive: ERR985064
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18968)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18968)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702225.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702225.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 20:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,045
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 27 of 67
            Pseudo Genes (incomplete)         :: 25 of 67
            Pseudo Genes (internal stop)      :: 19 of 67
            Pseudo Genes (multiple problems)  :: 4 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18968
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304449-rid9560453.denovo.054"
                     /strain="VREN0534"
                     /db_xref="taxon:1352"
     gene            complement(341..2311)
                     /locus_tag="IXT20_001879"
     CDS             complement(341..2311)
                     /locus_tag="IXT20_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4860317.1"
                     /translation="MEIMGRSQLLNFKKKRKTKKEQKTRNSKVVNNTFVSDLTSLVGY
                     QFTPSYIRTGKRWGTIVKLVNTYGMNRNYGFGWMVKAIPEVRVEGVKAYLIEADKPVS
                     QKDQQEIMHENVGGSTNSYENNLEGKNSDAEISKTVMRIMDLNTASLLDSMANKIIDW
                     KLEILLVSDSPNKISEQLIKLQRLYDEKMYGLKLMSYGGDQRDLFDRLLSEPIGNVYE
                     YTSMSSDFAGNDHAIRKGLDDANGWAIGTMSTSYASGVAFMALDDSFTSKVLVASHKN
                     ATIKGYDEKISASSLWGQLIANNAMANHKRTFHIVLNKFDYFGDPTGRTFVAKPSLNS
                     VIEKVNLAEEGLNSLELFGNQKDIGDLYRRNLDKLTQIFYLLAGRTLDSATRVLLKKE
                     LGNFYISQKLWDINYEERSKLLVNSSDTMPVLGDFISYLTNAETKAKKSGTEKEVDRI
                     IVLKDTLRTALDSFSRMFNQRTTLKEIGKSDKLQFYYNLGDLRAETNVMEAQFLNTFD
                     FITSQAKKDDIVMIHGFDKLSIETVGLIKKRLTMLEEDGIRLAFLFDTIGSGEDNEYK
                     EDIETADIFNTDGKIYQSFESDFNYTIIGKMTSGELMKYEKKVAPARKGEDDQKLSVD
                     LRQYLTTGRSNKFQIRRPVDLTNNFVIGEFIL"
     gene            complement(2326..3192)
                     /locus_tag="IXT20_001880"
     CDS             complement(2326..3192)
                     /locus_tag="IXT20_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4860318.1"
                     /translation="MADNSNYGFKHNLHQKPSETIVRVEWLEKHVNSTYSVTAKRLIV
                     SAIVVAVVVLGSIVYLVSDTIKLKFIFVPTLLMIIGFLILISQYMFIDPSSGSLISGS
                     IRLFKSVVNSARVSMGKKKPFRGTEILKVKQDGTIVYRDGSVALMFNLDGATSLTAFP
                     EEVMAQESKSIQYQNTRDRSLAEIKITSSQPQNTERQTRNMKIQKRNARSNKAVFDII
                     DQQERFVSEYIDGVKTTVIQYLILKSQSKMVLDEGVERLENYTSRGLYYSVTQLSAKQ
                     TKEILSDIYALK"
     gene            complement(3182..3415)
                     /locus_tag="IXT20_001881"
     CDS             complement(3182..3415)
                     /locus_tag="IXT20_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAQ4860319.1"
                     /translation="MREEKIVYPVIQNNQENMTSSQHLQEAQLVYQVNELPQTNETMS
                     KSISLIGLLIVAIVITVGVYRKLNVKGSISDGR"
     gene            complement(3427..5583)
                     /locus_tag="IXT20_001882"
     CDS             complement(3427..5583)
                     /locus_tag="IXT20_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4860320.1"
                     /translation="MAKFKDITESINYVAETVPVVALSLKPQLLEVYKKEEKEAKAKK
                     AKAKVWKEFDALLTKDLPKKNYMVIEDVQKLNSEGVIDEDDAILDSEKYVVLLKSSLN
                     YVADLPEKFKEKLLAGVRFMDSSGQLLVIPDDPNVFESEVEEATDFYSAVEQLEVATN
                     GLERIAETFNSSEEVEFEAVAENVPDEKEALFVDDNKPTEAENQEVSIEETQIQTETY
                     QQPKIDEKPESQSDKENYVYEAEKITSESDVPKNLDADFLEKNFEQIIADKFQPVVLD
                     EINDPVDLNEEMLSIPELKPLLFLAKESMNARISIANNKINSELNKTKEQLILKGKQM
                     LADELLRIEEATSLNGQNQFTNTLSVAKESYEAEASKIDDLVMEKSNIIRKDYDMAKQ
                     AYLDAELKKLEQRYDAEHLPEVKQQEDEYRKATEESLDQQYQEAIESIKNDALKQSQE
                     AKEVVVERIINSLKPEIEKSLTDVQEKTLEIVEKTKSENEAEFHDTKEKTTDVVVKYF
                     ANKEEVEKQVANNTKELETKLVEQQKTIEEYKDRMKEVKDLAEGYKLNSEKLAEQSEA
                     LREDLSRSRSDLAQMAEKTKSLAVATENQSSSKRDFTSKKMATIISVAAMACGTILGS
                     SYFISHNNQQAQAQTQTSISRVENSIGTNTNSASTNSSYEATPTTEFKVGKDVVISID
                     GKTTKAEVISIDGEKAVVKTPDGNQYKIQAHLSDNN"
     gene            complement(5603..5824)
                     /locus_tag="IXT20_001883"
     CDS             complement(5603..5824)
                     /locus_tag="IXT20_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4860321.1"
                     /translation="MVFDREKMLAHANEVLMSSLKGTELAKIMNMNVNQFYDYRNGSK
                     KIEKARLETLIKFEKAYVYMLDKQKRTID"
     gene            complement(5934..6272)
                     /locus_tag="IXT20_001884"
     CDS             complement(5934..6272)
                     /locus_tag="IXT20_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4860322.1"
                     /translation="MFDSIKNLVSGMTPDMKTLVQVVLYFLAFVFSLKPGFTAMKFFG
                     DAKWGDGLKYVGAIIAIFIIPVITTVMLYTMGTKTGNDLNQKANIISALIPVVGIYLV
                     SKFPKNELLG"
     gene            complement(6672..10313)
                     /locus_tag="IXT20_001885"
     CDS             complement(6672..10313)
                     /locus_tag="IXT20_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4860323.1"
                     /translation="MKFKKYLLQNLRIKRWVVRGLVIGGVLAVTLGAPFGMNDPTPDN
                     QDSKQETVINKNVAYAESKPAEQGMWTLYARILMGDKEVNDSAAKTGQMNLLGQGGIS
                     ADFPYSDISKQGNSIAGGNAGTQLASTLATYSYYGYIETVSGNAIASQASSILGSIGR
                     TIGSFIGSISLILSNIAESINELLGKFFMSMNIFSLMGWGEGGSDVSNPVSKALTSLL
                     NNLGLNNELFKAIASLSMLFFTAILGYQGMKLLSKDGVNSKGLSPVLEKWIVRIFVLF
                     IFLPVAGSLLGVYINGIQSAKKDGDIPTNVVSDYIINTRLWASTQNLAPSSDTGGDVP
                     DGKPTSEIKFVDTDYEPSKKSAMIRAINTSSYARQNNDLDSNSVGMALLKEWSSNDTF
                     NINTYAGDVNVAENDAYMGRAPAVWTGYNLGDGKEGSAIKKPTNLNGYIWSANQNVTD
                     DNRKPDGKGTAHNFDATQPWGVANNATFSTQSVALLLQSALDTSGAKFYAYNIAPSGI
                     QGSAKNMSTVKTEWRTASLVGHGAMGKFGSWLAMNAESIVLAVLYLACAWAMFNVNIV
                     KAFKREFIRIWEVVLTGSLASGAGVMAISLGVVTTSLIGLQLPIFLITFIRAFGDTAV
                     GFIPNTLVGDGVANIVRSAMILGVGYLLVFKKGEKVNNLINMLIGLPLEVAFGFDERA
                     KLLFGNRNDAVRVVDGVSKAIKKSQGAKNPYYDGGNSSVKDMVAGGLGAVGAQKMAER
                     LNNMGDSNADAKSIADKVIPETPEGMSFNGGGVGSTINNTSFKDLVRQDQLRNNSQQY
                     IDHLGRMVDENGNFVDENGELLKDSKYGKDAKGNFIDEKGRLVDEDGNLIDTNGKPLE
                     DSEDGNIGMDSEGRLIDEKGRLVDEDGNLVDENGRRLLNGDFGQDSNGRLVDEKGRLV
                     DEDGNLVDEFGNKLGKHSDGYNVNGDLVDEDGNIIETRDALVADRNRELEQQRHTPIA
                     VAIPENEDDEITLDQTHQELRNEMSGEDVSDRVHRELGDGASGESQSRELNSNFDNSA
                     IRNDTALKDRESLTKETDKEQSDVKKKFIPTPNVNQTDKYETNIGSGMSKDNQSIREP
                     ELTNKNNRTNNSKSTSDNSGKSNQSTKKPSTAKSAVEYLSKTGKNVYTSADKALAKAP
                     KGSLAGITYEAKNEAIKIGKGTAKVAKATATGASKTGKVVKKSTMSVKDYIVKYGNQK
                     STENKSEDE"
     gene            complement(10316..12265)
                     /locus_tag="IXT20_001886"
     CDS             complement(10316..12265)
                     /locus_tag="IXT20_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HAQ4860324.1"
                     /translation="MENSTEFFKNIQTEFNHALTPMKPPKHKLVGRDDELHELSLILN
                     RPETPQAILLGEAGTGKTALASEWAERQRQDGKKVQVYKLSIGQLRVGGDDVLQTRLE
                     TLIPRLKEYERILKAEDKDNEFVLFIDEFHKVVSSFGSGNKIGGDSLKEPLANESLQM
                     ITATTRKEYENYIKRDDALGRRLQPLELNELKSDVVVDILRQWLNTSSVKDDPQASFR
                     LSQRIPDELLQEIVQANKLYREDLSEPAKSIAVMEDLIAKDRVDGNAYNRESLLQIFG
                     KYNIQLDSDFDMEKLQEELKSIKGQPLAVYTITMLFKRMKLGFDRFKGRKQPKATALF
                     TGSTGVGKTAMAKAVARGLYGDENKVLLVNMPDYKTKDDAMRFRRLIGTHVRHDPSAI
                     IVCDEYEKAGVETLDAFLSILDEGRISFTERGWDDREVHDTVSLSNTILFATTNAGAE
                     MFDSLNRYSSLSRVTNKENSNEYSTELKREYRKVASGIKQALIGEGLKPEVINRFGRI
                     VPFWALSEQTLLEIAYNEMLKSAELVRKHLNIDLRLPPRKERVYGQNFYADDLSMHLV
                     FDMASQEDSKSGGARNILHLVESEFEEAITEAIITYPNAKIFDVKLSEAGAFEGKGDS
                     NAEIIAKPSVAESKENKIVFSIGGA"
     gene            complement(12307..13365)
                     /locus_tag="IXT20_001887"
     CDS             complement(12307..13365)
                     /locus_tag="IXT20_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliS"
                     /protein_id="HAQ4860325.1"
                     /translation="MEKNVNSTSTKDQYSIIGGLLGDKDKRTRHGLRIMLATFLGVLF
                     LLTGLGSYIYQLGNYNYYLVNNTTPIGSSLEFARSGAEITISDVWTDQNRDVTVVKLG
                     YSQNARKLLSTQGKNYNLYMATKEKDRPKKLEMSYGMLSTDGDAFLFLKGKLEERAYQ
                     VFIANQVSLISGDDTADDDTKVNVNEADSVTKALSQYSMNDVNNEGVISFGKKAKPVN
                     TPDNINFRINPYSDSTNVYQGSFLTKDGDIDYSKVVAVTSKNNVVNKLEDQLNASNTN
                     IKKIEATKAEYLERLQQNPDDKDSQESIRQLDSRMETEIKAINDTQKLIDQYKNADFN
                     KDSFGDMKTTFKYLTLTD"
     gene            complement(13484..13939)
                     /locus_tag="IXT20_001888"
     CDS             complement(13484..13939)
                     /locus_tag="IXT20_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4860326.1"
                     /translation="MNAINWHEFVNYMESLLDDLKSFSIADAVKHGLPVSTTMKLRGL
                     KIPDEIIKLRTKTIIETAKVVDNMNHELKGKKTTYEKDFERFLKSVIGKYLGKKSFSE
                     LRDEEIVPTPMVSKIRKNKAEGNYYLGTIAKIIDYLENKEQKKGTKKGR"
     gene            complement(14523..15326)
                     /locus_tag="IXT20_001889"
     CDS             complement(14523..15326)
                     /locus_tag="IXT20_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HAQ4860327.1"
                     /translation="MSQEVTEKIIDSDVDLVSEQTNAIVRYNNGLNTTALRGFKPVEM
                     DVFWAILSKMKRQGTAEVTFDFNLFRYLVKYDRREKDSFYSVLKALSDKLGTLTYRFE
                     DSDVYEQLWLFQKFRIDKQKETVTIKANEEFEFILNSIGKNFTRFELDNFTKLRSSYT
                     KELYRHLMQYRNKEWRNGYWVVKVEDFRRALSIPSSYRMTNIDKRILEQAKEEFLAPD
                     ENGIQVFETFDYEKIYAKKGRRVDRLRFTFSEPESNLPTISMHNWLEED"
     gene            16306..17529
                     /locus_tag="IXT20_001890"
     CDS             16306..17529
                     /locus_tag="IXT20_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HAQ4860328.1"
                     /translation="MESYQFQIFKLVPSLERRGNRMKITEKNVNPKFEPTESNLKKFD
                     LTVTLSNQLVDSIVEMDAMPQKIYLYAIGHLDPKNPPFNNTVKVSKKALFALMSSSSS
                     SSKHSRLKQVIEKLHQQSVIKIQRTKPNGKTEYKVISPLEITEFNDFSDAVSIKFSSS
                     IMPLLTNLEDHFTQYTFDQVVNLTSKYAISFFQLFTKDFNLYEANRDNLTWSTKSLDR
                     LKNPVFSIADLRKKFSIEKKYKAFRDFERRVIKEPIAQLNKYTDFKIDYELIKKGHTY
                     TNIKFLLTRTMSVPVPYKQEQRDPAYLKGAEDKTEKMNQSYLKAMNSKYTKLLIKNFL
                     ISSADLCDLKLMSRLQNKVYPLYDRLSKDRGFESVVNHLSYVATHKEDYTIKNTVKYL
                     EKSITSYLPYVNLYD"
     gene            17564..18331
                     /locus_tag="IXT20_001891"
     CDS             17564..18331
                     /locus_tag="IXT20_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HAQ4860329.1"
                     /translation="MKKDELTKYNEEFLATIDMKMDLKSYELYQLLFSYISPTKLKKT
                     GVVDLPVKAIINYMDVDQNLETELKTIVNTLQRQAVYEISNLDKKYKTYTTVSAIDRI
                     HWDLEEHSVSFSFSEDVIPLIVSIKKKYPFFVFEDLASFQKKYSIVLYKYLAMYYKKY
                     LIAKNKGDFEKAEIYKNPLIEIVKIREITKTENEYKTISNFDRRVLRESVEEINEKSM
                     LNIDFAKVRKGRNYNFAVQFNISSEFDEKTYREKKLG"
     gene            18523..18921
                     /locus_tag="IXT20_001892"
     CDS             18523..18921
                     /locus_tag="IXT20_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin HicB"
                     /protein_id="HAQ4860330.1"
                     /translation="MKYFFCAVFEWKGNEYAVKFPDFPDLYTHGETLEDAFEMAQEVL
                     EGYLVLLELQEKEIPSPTPVQELNARIKRSENVNLFVVKANTKVAKARNKQNYDRKSV
                     TVEHYLNVLAAEENINLSKALNDLLREKLI"
BASE COUNT         5731 a         3616 c         2754 g         6867 t
ORIGIN      
        1 aactctcgct ttttcttcat cagtaaagtt tttatacaac tgattacaat aatgaggttt
       61 ttgaagtttc tgaataccta tactaacttc aaatccttct tcatgttgaa ttaggattac
      121 ttctgtgtct ctacctagaa atattcgatc taaaattttt atgtttggtt tcattattta
      181 acctccacta taattatttc actcagtaag gtttcacttt atgaaatttc tatagttctc
      241 ttttcacaaa acgtaccatg ttctctaaga ctgtttttag tctcaaatat acatgttttt
      301 tcttatcctt tccttataca ctcaaaatca aagataaaac ttaaagaata aactctccaa
      361 taacaaaatt attagtcaga tccactggtc tcctgatctg aaatttatta cttcttcctg
      421 ttgtcaaata ctgtctcaaa tcaacagaaa gtttttgatc atcttctcct tttctagcag
      481 gagcaacttt tttttcatac ttcattaact ctccactggt catctttcca atgatagtgt
      541 aattaaaatc actttcaaaa ctttggtaaa tttttccatc tgtattgaat atatcagctg
      601 tttcaatatc ttctttgtat tcattgtctt ctcctgaacc gattgtatca aatagaaagg
      661 caagacgaat tccatcttct tcaagcattg ttaatctttt tttaattagc ccaacggttt
      721 caatagataa cttatcgaag ccgtgaatca taacaatatc atcttttttt gcttgtgatg
      781 tgatgaaatc aaaggtattt aaaaattgag cttccataac attggtttct gctcttagat
      841 cacctaagtt atagtaaaac tgtaatttgt cacttttacc gatttctttc agtgtcgttc
      901 tttgattaaa catcctagag aagctgtcta atgctgttct caatgtgtct tttaatacaa
      961 taatacgatc gacttctttt tcagtaccag attttttggc ttttgtttca gcattagtca
     1021 aatagctaat aaaatcccca agaactggca ttgtatcact actgttcacc aaaagttttg
     1081 aacgttcttc ataattaata tcccaaagtt tctgacttat ataaaagtta cctaattctt
     1141 ttttcaataa aactctagtt gcagaatcta atgttctgcc tgctaacaaa taaaaaatct
     1201 gagttagttt atcgagattc cgtctatata aatcaccaat gtccttttga ttaccaaaaa
     1261 gctccaaaga gttaagacct tcttcagcca aattaacctt ttctattaca ctgtttaacg
     1321 aaggtttggc aacaaatgtt ctaccagttg gatcaccaaa gtaatcaaat ttatttaaga
     1381 caatatgaaa agttcgttta tgatttgcca ttgcattatt agcaatcaat tgcccccaaa
     1441 gtgatgatgc tgagattttt tcatcatagc ctttaattgt agcgtttttg tgtgatgcca
     1501 ctaaaacttt acttgtaaaa gaatcatcca gtgccataaa agctacacca cttgcgtagc
     1561 tagtagacat agtgccgata gcccatccat tcgcatcatc taaacctttg cgaattgcat
     1621 gatcatttcc tgcaaaatcc gaactcatag acgtatactc atagacattc ccgattggtt
     1681 cacttaaaag tctatcaaac agatcacgtt gatctcctcc atatgacata agctttaatc
     1741 catacatttt ttcatcatac aaccgttgga gtttaattag ctgttcgctg attttattag
     1801 gagaatcgga tactaatagg atttctagct tccaatcaat aattttattt gccatgctgt
     1861 ctagtaaaga tgctgtattc aaatccataa tccgcataac agttttgcta atttcagcat
     1921 ctgaattttt tccttctaaa ttattttcat aactattcgt tgatccccca acattctcgt
     1981 gcattatttc ttgttgatct ttttgtgaca ctggcttatc tgcttcaatt agataggctt
     2041 ttaccccttc tactctcact tcaggaatag ctttaaccat ccaaccaaat ccataatttc
     2101 tattcattcc ataagtattc actaatttca caattgttcc ccaccgctta cctgtcctaa
     2161 tgtaagatgg tgtaaattga taaccaacta aagatgttaa atcactaaca aaggtattat
     2221 tcactacctt tgaatttcta gttttttgtt cttttttagt ttttctcttt tttttgaaat
     2281 ttaatagttg tgatctaccc ataatttcca tttaatctct cacctctatt tcagtgcata
     2341 aatatcagat aatatttctt ttgtttgttt agctgacaac tgagtgacac tataataaag
     2401 tcctctactc gtatagtttt ctaaacgttc cacaccttca tccaatacca tcttagattg
     2461 agatttcaag ataagatatt ggattactgt tgttttcaca ccatcaatat attctgatac
     2521 aaatcgttct tgctgatcta ttatgtcaaa gactgctttg ttactccttg catttcgttt
     2581 ttgtattttc atatttcgag tttgtctttc cgtattctga ggttgagatg aagtgatttt
     2641 gatttcagct aaggaacgat ctctggtatt ttgatattgt attgatttcg attcctgagc
     2701 cataacttct tctggaaaag cggttagtga tgtagcccca tctagattga acattaatgc
     2761 tacactccca tctcgatata cgattgttcc atcttgtttc acttttaaaa tctctgtgcc
     2821 tcgaaatggt ttctttttcc ccatagatac tcttgctgaa ttaactactg atttgaacag
     2881 tctaatagaa ccagaaatca atgaaccact agatggatca ataaacatat actggctaat
     2941 tagaataaga aatccaatta tcattaataa cgttggaaca aaaataaatt tcagtttaat
     3001 cgtatcagaa actaagtaaa caattgatcc aagaacaacg actgcaacaa ctatagctga
     3061 tacaattaat ctttttgctg taacagaata ggtgctattg acatgctttt caagccattc
     3121 aactctgaca attgtttcag aaggtttttg atgtaagtta tgtttaaatc catagtttga
     3181 gttatctgcc atcagaaata cttcctttca cgttcaactt acggtaaaca cctacagtta
     3241 tcactattgc aacaataagc aagccaatta aagatattga cttgctcatt gtctcgttcg
     3301 tttgtggtag ttcatttacc tgatacacga gctgtgcttc ctgtaagtgc tgagaagagg
     3361 tcatgttttc ttgattgttt tgaattactg gataaactat tttttcttct cgcataatag
     3421 ccctccttag ttattatcag ataagtgagc ttgaattttg tactgatttc cgtctggagt
     3481 tttcacaact gctttttctc catcaataga aatcacttca gccttagttg ttttcccatc
     3541 aatactgatg acaacatctt tcccaacttt gaactctgta gttggtgttg cttcatagct
     3601 actatttgtt gaagcagagt tcgtatttgt cccaatcgaa ttttccactc tcgaaataga
     3661 tgtttgagtt tgagcctgtg cttgttgatt attatgacta ataaagtagg acgatcccaa
     3721 gatcgttccg caagccattg cagcgacaga aatgatagta gccatctttt tacttgtaaa
     3781 atcacgtttt gaacttgatt gattttctgt agcaactgct aatgatttcg ttttttctgc
     3841 catttgagct agatcacttc gggaacgaga taagtcttct cttaaagctt cactttgttc
     3901 tgccaacttt tcgctattta atttataacc ctctgctaaa tctttgacct ctttcattcg
     3961 atctttatat tcttcgatag ttttttgttg ctctacaagt tttgtttcta attctttcgt
     4021 gttgttagct acctgctttt caacttcttc tttgttcgca aaatatttga caaccacatc
     4081 tgttgttttc tcttttgtat catggaactc agcttcattt tctgactttg ttttttcgac
     4141 aatctctaaa gttttttctt gcacgtctgt taatgatttt tcaatttctg gtttcaatga
     4201 attgataatt cgttccacca caacttcttt tgcttcttgg ctttgtttca atgcatcgtt
     4261 tttaatagat tcaattgctt cttggtactg ttgatcaaga ctttcttcag ttgcttttct
     4321 atattcatct tcttgttgtt taacttctgg taaatgctca gcatcatacc gttgctctag
     4381 tttctttagt tcagcatcca aataagcctg tttcgccata tcataatctt tacgaatgat
     4441 attggatttt tccatcacta aatcatcaat tttagatgct tctgcttcgt agctctcttt
     4501 agcaaccgat agtgtatttg taaactgatt ttgaccattt aatgaggttg cttcctctat
     4561 ccgcaaaagc tcatcagcta acatttgctt tcctttgagg atcaattgct ccttcgtctt
     4621 atttaattca ctgttgattt tattattagc aatagagatg cgagcgttca tactctcttt
     4681 agctaaaaac agcaatggtt tcaactcagg tatggatagc atctcctcgt ttaaatcgac
     4741 agggtcatta atctcatcta aaactacagg ttgaaatttg tcagcaatga tctgttcaaa
     4801 atttttttct aaaaaatcag catctaagtt tttcggtaca tcactttcag atgtgatttt
     4861 ttcagcttca taaacataat tttctttgtc actttgggat tcgggttttt catcaatttt
     4921 tggttgttgg taggtttctg tttgtatttg tgtttcttct atgcttactt cttggttttc
     4981 tgcttcggtt ggtttgttat catccacaaa aagagcttct ttttcatctg gtacattttc
     5041 tgctacggct tcaaattcaa cttcttcaga agagttgaag gtctcagcaa ttcgttctaa
     5101 tccattagta gctacttcta attgctctac tgcgctatag aagtctgtag cttcttcaac
     5161 ttctgattca aacacattag gatcatccgg aataactaat aattgaccac tactatccat
     5221 aaaacggaca cctgctaata gcttctcttt aaatttttct ggtagatcag ctacataatt
     5281 caaacttgac ttcaatagaa cgacatactt ctcagaatcg agaatagcat catcctcatc
     5341 tataactccc tcactgttga gcttttgtac atcttcaatt accatataat ttttctttgg
     5401 taaatctttg gtcagcaacg catcgaattc tttccatacc tttgctttag ctttttttgc
     5461 cttcgcttct ttttcttctt ttttgtacac ttcgagtaat tgtggcttca aagagagtgc
     5521 aactacagga actgtttctg ccacataatt aatagattcg gtaatatctt tgaattttgc
     5581 cattattgta aaactccttt tttcagtcaa tagttctttt ctgtttatct agcatatata
     5641 catatgcttt ttcgaattta ataagcgttt caagtctggc tttttcaatc tttttactcc
     5701 catttcgata atcatagaac tggttaacat tcatattcat tatctttgcc agttcggtac
     5761 ctttcagact gctcattaat acttcgttgg cgtgagctaa cattttttct ctatcaaaaa
     5821 ccatcttaca tttactttct tccctcactt ctaaactacg cttggagcgt agttttttgg
     5881 taaaaagtaa attgaacaaa tcggcatatt atccaaattt gttcaattca cttttaacct
     5941 aacagttcat tttttggaaa ttttgaaact agatagatac caacaactgg aataagtgct
     6001 gatatgatat tagctttttg gttcaaatca ttacctgttt tagtacccat tgtgtaaagc
     6061 ataacggttg taattacagg aataataaag attgcaataa tcgccccaac atattttaat
     6121 ccatctcccc attttgcatc accaaaaaat ttcattgcag tgaatcctgg tttcaacgaa
     6181 aaaacgaatg ccaagaaata caaaacaact tgtactaaag ttttcatatc aggcgtcatt
     6241 ccgctaacca aatttttaat agaatcgaac atataaaaac ctccttttta atatttttaa
     6301 taagttaatt atatcatcga caaatcttat ttgttcactt gtcaatgatc taactaagaa
     6361 aaagacgtct cgatatttac atctttatgt tatcataata acataaattt tcgatgatta
     6421 aaagagattt tccattaaat tatttcactt tttttattgg ctaaaaatgc tggtttatca
     6481 agtttttgtt tagataataa ttctgttctt taccagcata agcccttttt aattatacaa
     6541 ctttgaacag taaatattta attcatttat agacaattta aaataatttt tttgcataaa
     6601 tagaaatttt ccttgacatc ttcagttaga aatcaaagta aaaagaagga gcaaatcaat
     6661 gctccttctt tttactcgtc ttcagactta ttttcagttg atttttgatt accgtatttt
     6721 acaatatagt ccttgactga catggtagat ttcttcacta ctttaccagt tttggatgct
     6781 cctgtagcag tagccttggc aaccttagcc gttcctttac ctattttaat agcttcgttt
     6841 ttagcttcat aggttatccc tgctagacta ccttttggag cttttgctaa agctttatct
     6901 gctgaggtgt aaacattttt accagttttt gatagatatt caacggctga tttagctgtt
     6961 gatggtttct tggttgattg attagatttc cctgaattat ctgaagtaga tttagaatta
     7021 tttgttctat tatttttatt tgtcagttca ggttctctaa ttgattgatt gtctttcgac
     7081 attcctgatc caatatttgt ttcatattta tctgtttgat taacattagg agtaggaatg
     7141 aactttttct tcacatctga ttgttcttta tccgtttctt ttgttagcga ttctctgtct
     7201 ttaagtgctg tgtcattccg aatagcacta ttatcaaagt tgctgttaag ctctcttgac
     7261 tgactttcac cagatgctcc atctcctaat tcccgatgca ctctgtctga cacgtcctca
     7321 ccactcattt cattcctcag ttcttgatga gtttgatcta aagtaatctc gtcatcttca
     7381 ttctcaggaa ttgcaacagc aattggcgta tgtcgttgtt gttcgagttc tctatttcga
     7441 tctgccacaa gcgcatctct cgtctcaatg atattcccat cctcatcgac taaatctcca
     7501 ttcacattat atccatcgct gtgtttacca agtttattac caaactcatc aacaagattt
     7561 ccatcttcat caactaaacg ccctttttcg tcaactaaac gtccgttact atcctgacca
     7621 aaatcaccat ttagcaacct gcgtccattt tcgtcaacaa gatttccatc ttcatcgact
     7681 aagcgacctt tttcatcaat taaacgtcct tcgctatcca taccaatatt accatcttca
     7741 ctatcttcca atggtttacc attagtatct ataaggtttc catcttcatc gaccaaacgc
     7801 cctttttcgt caataaagtt gcctttagca tctttaccgt atttactatc ttttaataat
     7861 tcaccatttt catcgacaaa gtttccgttt tcatcaacca tacgtcctaa atgatcaata
     7921 tattgttggg agttattacg tagttgatct tgacgaacta aatctttgaa agaggtatta
     7981 ttgattgttg aaccaactcc gccaccgttg aatgacattc cttctggtgt ttcaggaatt
     8041 actttatcag caatggactt agcatcagca ttgctatctc ccatattgtt caaacgttca
     8101 gccatttttt gcgctccaac tgctccaaga cctcctgcaa ccatatcttt tactgagctg
     8161 ttgccaccat cataataggg atttttagct ccttggcttt tcttgattgc tttagaaacg
     8221 ccgtccacaa ctcgcacagc atcatttcta ttaccaaata ataacttagc acgttcatca
     8281 aacccaaacg ctacttcaag tggtaagcca ataagcatat taatcaaatt atttactttt
     8341 tcgccttttt taaagactaa aagatatcct acacctaaaa tcatagcaga tcgaacaata
     8401 tttgctacgc catcgccaac taatgtatta ggaatgaatc ccacagcagt atcaccaaat
     8461 gcacgaataa aagtaatcag aaagataggt aattgtagtc cgatcagaga tgttgtaaca
     8521 acacctaatg aaattgccat cacacctgct cctgatgcaa gtgatcctgt tagaactact
     8581 tcccaaatcc gaataaattc acgtttaaat gctttcacga tattcacatt gaacatcgcc
     8641 caagcacatg ctaagtaaag aactgctaaa acgattgatt cagcgttcat cgctagccac
     8701 gagccaaact tacccatagc tccatgacca actaaggatg ctgtacgcca ttctgttttt
     8761 actgttgaca tattcttagc actgccttga attcctgaag gagcgatatt atacgcataa
     8821 aacttagctc ccgatgtatc aagtgccgat tggagcaata aagctaccga ctgtgtgcta
     8881 aaagttgcat tgttagcaac gccccaaggt tgagtagcat caaagttatg cgctgttcct
     8941 tttccatcag gcttgcgatt gtcgtctgtc acattttgat ttgctgacca aatatatcca
     9001 tttaaattgg ttggtttctt aattgcactt ccttcttttc catcccctaa attatagcct
     9061 gtccaaacag caggcgctct tcccatataa gcatcatttt cggcgacatt aacatcaccg
     9121 gcataagtat taatattgaa agtatcattc gatgaccatt cttttaatag agccataccg
     9181 actgaattag aatctagatc attattttgt ctggcataac tgctagtatt gatcgctctt
     9241 atcattgcag attttttaga tggttcataa tctgtatcaa cgaacttgat ttcggatgta
     9301 ggctttccgt caggtacatc accaccagta tcagatgatg gagcaagatt ttgagtagaa
     9361 gcccaaagtc ttgtgttaat gatgtagtca gaaacaacat ttgttggtat atctccatct
     9421 ttttttgctg actggatgcc gttgatataa actcccaata gcgatcctgc cactggtaag
     9481 aatataaaca atacaaaaat acgaacaatc catttttcaa gtactggtga taatcccttc
     9541 gagttaacac catctttcga caataatttc attccttgat aacctagtat tgctgtgaaa
     9601 aagagcatag ataagcttgc tatcgcttta aataattcat tatttaatcc caaattgtta
     9661 agcaagctag ttaatgcttt agataccgga ttagatacat ccgatccacc ttctccccac
     9721 cccataagag aaaatatatt catactcatg aaaaattttc caaggagttc atttatggat
     9781 tcagcaatat tagaaaggat taaactaatt gatccgataa aactaccaat cgtcctacca
     9841 atacttccta gaatggatga tgcttgactg gcaatagcat ttcctgagac tgtttcaata
     9901 tatccgtagt aactataagt tgctaaagta gatgctagtt gagtacctgc attaccccct
     9961 gcaatcgaat taccttgttt agaaatgtca gaataaggga aatcggcact gataccacct
    10021 tgtcctaaca aattcatttg tccagtttta gcggcactat cattaacttc tttatctccc
    10081 attaggatac gagcatatag tgtccacatt ccttgttctg ccggtttact ttccgcatat
    10141 gcaacgttct tatttataac tgtttcttgt ttgctatctt gattgtcagg agttggatca
    10201 ttcattccaa aaggagcgcc taaagtaact gctaatactc ctccaataac tagacctctt
    10261 acgacccatc gttttattct aaggttttgt agtaaatatt tcttgaattt catagctacg
    10321 cccctccaat cgaaaataca atcttgtttt ctttactttc agcaacggaa ggctttgcaa
    10381 taatttcagc attagaatca ccttttcctt caaacgctcc ggcttcactc aacttaacat
    10441 cgaatatttt tgcgtttgga taagtaatta ttgcttcagt aatcgcttct tcaaactcag
    10501 attcaactaa atgcaagata ttccttgcac cccctgattt agaatcctct tgtgaagcca
    10561 tgtcaaaaac taagtgcatg gagagatcgt ctgcataaaa attttgacca tatactcttt
    10621 ctttcctagg tggaagacgt aaatctatat ttaaatgttt tcgtactaat tcagcactct
    10681 tcaacatttc attataagca atttccagaa gagtttgttc ggataaagcc caaaatggaa
    10741 ctattcgtcc aaatcggtta ataacttcag gctttagacc ttcaccaatc aaggcttgtt
    10801 tgatccctga tgcgactttt cggtattctc tttttagttc agtgctgtat tcattagaat
    10861 tttccttgtt tgtaacacga gatagtgagc tatatcggtt taacgaatcg aacatctccg
    10921 cacctgcatt tgttgtcgca aataaaattg tatttgacaa tgaaacggtg tcgtgaactt
    10981 ctcgatcatc ccatcctctt tcagtaaaac ttatcctacc ttcatctaaa attgataaaa
    11041 aggcatcgag tgtttctaca ccagctttct catattcatc acacacaata atcgcagaag
    11101 gatcatgcct tacatgagta cctataagtc gtctaaaacg cattgcatca tctttggttt
    11161 tgtagtccgg catattaact aagagaactt tattttcatc gccataaagt cctcttgcca
    11221 cggctttagc cattgcagtt ttaccgaccc ctgttgatcc tgtaaacaga gcagttgctt
    11281 ttggttgctt tcgaccttta aaacgatcaa atccaagctt catacgttta aacaacattg
    11341 ttatagtata aactgctaac ggttgtcctt ttatggactt taattcctct tgaagttttt
    11401 ccatatcaaa atcagaatct agctgaatat tatacttacc aaatatttgc aatagacttt
    11461 ctcgattata agcatttccg tctactcgat cttttgcaat caaatcctcc attacagcaa
    11521 ttgatttagc cggttcggac aaatcctcac gataaagttt gtttgcttgt acgatttctt
    11581 gaagtaattc atcaggaatc ctttgcgata atctaaaaga agcctgagga tcatctttaa
    11641 cagaggatgt atttagccat tgtctcaaaa tatcgacgac aacatcgctt tttagttcat
    11701 tcaattctaa cggttgtagc cgtctcccta aggcatcatc tcttttgata tagttttcat
    11761 attcttttct tgtagtagcg gtaatcattt gaagtgattc atttgctaac ggttctttta
    11821 gcgaatcacc cccaatttta ttgccactcc caaaagagct aacaacttta tgaaactcgt
    11881 caatgaagag gacaaattca ttatctttgt cctcagcttt taaaattcgc tcatattctt
    11941 tcaatcttgg aattaatgtt tctaaacgtg tttggaggac atcatctccc ccaacacgca
    12001 attgtccgat tgataattta tatacttgta ctttctttcc atcttgtcgc tgtctttcag
    12061 cccattcact tgcaagtgct gttttacctg ttccggcttc acctaacaag atagcttgcg
    12121 gtgtttccgg tctattcaag atcagtgaca actcatggag ttcgtcatca cgtcccacta
    12181 acttatgttt tggtggtttc attggtgtga gagcatgatt aaattcggtt tgaatattct
    12241 taaaaaactc ggtactattt tccatctgtt tgtgttcact tcctctaaaa taagaataat
    12301 ttacttctag tcagtcaaag ttaggtactt aaatgtcgtc ttcatgtctc caaaagaatc
    12361 tttgttaaag tcagcatttt tgtattgatc aatcaatttt tgtgtatcat tgattgcttt
    12421 aatttcggtc tccattcgac tgtctagttg acgaatactt tcttgcgaat ctttatcgtc
    12481 aggattttgt tgtaaacgtt ccagatactc agcttttgtg gcttcaattt tcttgatgtt
    12541 agtatttgaa gcatttaact gatcttctag cttatttaca acattattct tacttgtaac
    12601 tgctacaact tttgaataat caatatcgcc atctttggta agaaagcttc cttgatagac
    12661 attagttgaa tctgaatatg gatttattct gaaattgata ttatcaggag tattaacagg
    12721 ttttgctttt ttcccaaaag aaattactcc ttcattatta acatcattca tagaatattg
    12781 agaaagtgct tttgtaactg aatctgcttc attcacattg actttggtat catcgtcagc
    12841 tgtatcatca ccagaaatta gtgatacttg gtttgcaata aaaacttgat aagctctttc
    12901 ctctaattta ccttttagga ataagaaagc atccccatca gtacttaaca tcccatagct
    12961 catttcaagc ttcttaggac gatctttttc ttttgttgcc atatataagt tatagttttt
    13021 tccttgcgta gataacaact ttctagcgtt ttgggagtaa ccaagtttta ctactgttac
    13081 atctctattt tgatctgtcc acacatctga aatagtgatt tctgctcctg atcgagcaaa
    13141 ctccaatgaa gaccctatag gtgttgtatt gttcacgaga taataattat agtttcctaa
    13201 ttgataaatg tagctaccca agcctgttaa taaaaaaagc actcctagaa aagttgcaag
    13261 cataatgcgt aatccgtgac gtgttctctt atccttatca ccaagcaacc ctccaataat
    13321 actgtattga tctttggtag atgttgaatt gacgtttttt tccatcagtt tgtctgcccc
    13381 ctttcataaa agagcaaaca atcagacgtt ttttcggtaa ttctaggttc gggattttga
    13441 atcatttcgg tatattcttc cccattaata tttaacacaa aaattatctt cctttcttcg
    13501 ttcctttctt ttgctcttta ttttccaaat aatcaatgat ttttgctatt gtaccaagat
    13561 agtaattccc ctcagctttg ttttttcgga ttttgctaac cattggagta ggtacaatct
    13621 cttcatctct caattcactg aaagattttt tacctagata ttttccaata acagatttta
    13681 aaaaacgttc aaaatctttc tcgtaagttg ttttcttgcc ttttagttca tggttcatat
    13741 tgtcaacgac ttttgcggtt tcaataattg ttttagttct cagcttaata atttcatctg
    13801 gaattttcaa tcctcgtaat ttcatcgttg tactaactgg taacccatgc tttactgcat
    13861 cagcaatact aaaacttttt agatcatcta gtaagctctc catatagtta acaaattcat
    13921 gccaattaat agcattcatt gtctcacccc ttcatttttt taaaatgagg ttcgggcgta
    13981 acacatttat gttattataa tatcataata acataaataa atcaatcagc aaatttagat
    14041 atcccttaaa gtatcagcaa ttattcaaca agatatttat tttagctatt tcagtcatca
    14101 taaaagacta attagcaata aaataactaa tattatccat ttatcagacg aaaagaaaaa
    14161 acttatctaa ttactctata ttaactttta acaaatagtt gacttgtcca aaaatgttac
    14221 cttcaatctc acaaagatat taagatgttt cctaccaaaa ctgatatcac cggacttttt
    14281 attctgtgtt gtccaaaagt gttaccttta gtcgatcaac aaattaaaaa aaattgtttt
    14341 tttcttgact tgtccaaaag tgttacccat agttgttaaa atttgtatag attagccatt
    14401 ctttcgaata ttatttagac tgaaacttgc ttacaaaaaa acttcttgca tttattgtcc
    14461 aaaagtgtta cccatacttt tttaaagaag caggttgtat taaaattaca acctgcttct
    14521 tctcaatctt cttctaacca attatgcatt gaaatggttg gcaagtttga ttctggctca
    14581 ctaaaagtga atctgagccg atcaactctt cttcctttct ttgcatagat tttttcataa
    14641 tcaaaagttt caaatacctg tattccgttt tcatcaggag ctaaaaattc ttctttcgcc
    14701 tgctctagta ttcttttgtc aatatttgtc attctataac ttgaaggtat acttaatgct
    14761 cttctaaaat cttcaacttt gactacccaa taaccatttc gccactcttt atttctgtat
    14821 tgcatgagat gacgatacaa ttcttttgtg tagctacttc ttagttttgt aaaattatca
    14881 agttcaaaac gagtaaagtt tttgccaatt gagtttaaga taaattcaaa ttcctcattt
    14941 gccttaattg taactgtttc cttctgttta tctatccgga atttttgaaa aagccacagc
    15001 tgttcataga cgtctgaatc ttcaaaacgg taggttaaag tacctagttt gtcactcaaa
    15061 gcttttaaca cagaataaaa actgtccttt tctcttctgt cgtatttgac caaataacga
    15121 aagaggttga aatcaaaagt tacttctgct gttccttgac gtttcatttt tgataaaata
    15181 gcccaaaaca catccatttc aacaggctta aatcctcgca aagctgttgt attcagacca
    15241 ttgttataac gcacaattgc atttgtttgt tctgatacta aatctacatc actatcaata
    15301 atcttttcgg taacttcttg agacattgat tactctccaa tcttcttttt gcgctccttt
    15361 aatcatgtta atctggtact aattaaagga aacaattttt tctgcttgtt acctaaatta
    15421 aatcacaact aaaggtaaca gtcaacaaag atttgttact aatctaattt tctatttttt
    15481 cgccttccaa atcaagaaaa ccgaaggtaa cgtcttatcc tagtgtgata cctcaaatgt
    15541 ccacttttgt tacctttgct gtccaaaagt gttaccctta ctgtccactt ttgttacctt
    15601 tactgtccaa aagtgttacc tttatacccc tttaatactt actctcccaa gagattaagc
    15661 ctcccctaac agatatatta acagatattt aacagatata ttaacagata tttaataaag
    15721 gaggattact cctctcttat ttcaaaatct taaaaataaa aaatcttaaa aaaaactaac
    15781 actatctagg agtttatttt gcttgtgtaa ctttgaataa ttgaaaaaaa ggaaaaggag
    15841 gaagcttcag cttccccttt tatttatatt tataatatat attttaatct ttaaaacttt
    15901 agcaaccttc aaaaaggctt tatatcaatc ttaaatagat tttagggtgg acataaagcg
    15961 atgcgaacat ggacaaaaag cgatgtgaat gtggacaaaa agcgatgcaa acgtggacaa
    16021 aaagcgatgt ggtattccga atatatccaa aaaaagaaaa agtatgtgaa tttatttcta
    16081 aaaactagaa aaatatttgt tttttaattt tggtaatttt tataatttcg tatttttttg
    16141 aaaaggattt ttatattgaa aatgttctaa aatagcgatt tctgctgtct tatcaatgaa
    16201 aattgattaa aaaaataaaa tgtgttttac agagattgag ttatgttata aatttacgat
    16261 acatttctta ttgtatcgta tcgaaaacag gtctatactt attcaatgga atcgtaccag
    16321 ttccaaattt ttaagctcgt accaagctta gaaagaagag gcaatcgtat gaaaataaca
    16381 gaaaagaatg taaacccgaa atttgaacct accgaaagca atctaaaaaa gtttgattta
    16441 actgttactt tatcgaatca acttgtagat agcattgttg aaatggatgc tatgccacaa
    16501 aaaatttatc tttatgctat aggacatctt gaccctaaaa atccgccttt taataatact
    16561 gttaaagtat ctaaaaaggc tttatttgcg ttgatgtctt ctagttcatc ttcttcaaaa
    16621 cattcaagat taaaacaagt tattgaaaaa ttgcatcagc aatctgtcat taaaattcaa
    16681 agaactaaac ctaacggtaa gactgaatac aaagtcatct caccgctaga aattactgaa
    16741 ttcaatgatt tttctgatgc agtatctatt aaatttagta gttcaattat gcctttatta
    16801 acgaacttag aagatcattt tacgcaatat acatttgatc aagtagttaa cttgacaagc
    16861 aagtatgcga tcagtttttt tcaattattc actaaagatt ttaatctgta tgaagcaaat
    16921 agagataact taacatggag tacaaaatca cttgatagat taaaaaatcc tgttttctcc
    16981 attgcagatt tacgaaagaa atttagtatt gagaaaaaat ataaagcttt tcgagacttt
    17041 gaaagacgtg taattaaaga accgattgcc caactgaata aatacactga ttttaaaatt
    17101 gactatgagc ttatcaagaa gggtcatact tatacaaaca taaagttctt gcttacacgt
    17161 accatgtcag ttccggtacc ttataagcaa gaacaaagag accctgctta tttaaaaggc
    17221 gcagaagata aaacagagaa aatgaatcaa tcgtacttga aagctatgaa tagcaaatac
    17281 actaaattac taatcaaaaa ctttttgatc agttctgccg acttatgcga tcttaaactt
    17341 atgagccgac ttcagaataa agtttatccg ctttatgatc gtttatccaa agatagagga
    17401 tttgaatcag ttgtcaatca cctatcttac gttgctactc ataaggagga ttacacaata
    17461 aaaaatactg ttaaatatct cgaaaaatca attacgagtt atttgcctta tgtgaacctt
    17521 tatgattaac aactgaaaaa atgcgtgaag gaaggaaaaa cttttgaaaa aagatgaact
    17581 gacaaagtac aatgaggagt tcttagcaac aattgacatg aaaatggatt taaagtcgta
    17641 tgaactttat caattgctgt tcagttatat ctcacccaca aagctaaaga aaacaggcgt
    17701 agtagattta cctgttaaag cgattattaa ttacatggat gttgatcaaa atcttgaaac
    17761 ggaactaaaa accatagtta acactttgca aagacaagca gtttatgaaa tttcgaatct
    17821 agacaaaaaa tataagacgt atactactgt atctgctatt gatcggattc attgggattt
    17881 agaagagcat agtgtatctt tcagtttttc agaagatgta attcccttaa ttgtctcaat
    17941 caaaaagaag tatccgttct ttgtttttga agacttagca tcttttcaaa aaaaatatag
    18001 catcgtgtta tacaaatatt tggcgatgta ttataaaaaa tatcttatcg ccaaaaataa
    18061 aggagatttt gagaaagctg aaatatataa gaatcccctg attgaaatag ttaaaattcg
    18121 tgagattacg aaaacagaaa atgagtataa aacaatatcg aattttgata gacgtgtttt
    18181 aagggaatca gttgaggaaa ttaatgaaaa atcaatgctg aatattgatt ttgctaaagt
    18241 tagaaaaggc agaaattaca attttgctgt ccagtttaat atttctagtg agtttgatga
    18301 aaaaacttat agagaaaaaa aattaggtta atgcaaaata tatcttactc gtttgaagat
    18361 gcttcagacg tatgctaaga tatatagtgc cttgggagca aggtattaat ctcctttaga
    18421 ggttaattag cttgtgatcg cctatcaatt tttttggtag gcgattcttt tttttgaaac
    18481 aaaaaagcaa aaattatttt ttaattagag aggtgcaatg cgatgaaata ctttttttgt
    18541 gcagtattcg aatggaaagg aaatgaatat gcagttaaat tcccagattt tccagactta
    18601 tatacacatg gtgaaacttt agaagatgct tttgagatgg cgcaagaagt attggaagga
    18661 tacttggtgc ttttagagtt gcaagaaaaa gaaattccat cgcctacgcc tgttcaagag
    18721 ttaaatgcaa gaatcaagcg atctgagaat gttaatttat ttgtcgtaaa agctaatacg
    18781 aaagttgcta aagctcgaaa caagcaaaat tatgatcgaa aatcagtgac tgtggaacac
    18841 tatttaaatg ttttagcggc tgaagagaat attaatctgt cgaaagcttt gaatgatttg
    18901 ttgagagaaa agttgatttg atgtctctca gatgatcata ttaagcaaaa acagcttgtt
    18961 ttaggacg
//
LOCUS       DACGLE010000051        18603 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0534
            SAMEA3304449-rid9560453.denovo.055, whole genome shotgun sequence.
ACCESSION   DACGLE010000051 DACGLE010000000
VERSION     DACGLE010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304449
            Sequence Read Archive: ERR985064
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18603)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18603)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702225.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702225.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 20:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,045
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 27 of 67
            Pseudo Genes (incomplete)         :: 25 of 67
            Pseudo Genes (internal stop)      :: 19 of 67
            Pseudo Genes (multiple problems)  :: 4 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18603
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304449-rid9560453.denovo.055"
                     /strain="VREN0534"
                     /db_xref="taxon:1352"
     gene            332..946
                     /gene="gmk"
                     /locus_tag="IXT20_001893"
     CDS             332..946
                     /gene="gmk"
                     /locus_tag="IXT20_001893"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010766465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HAQ4860331.1"
                     /translation="MSERGLLIVLSGPSGVGKGTVRKAIFESEDNDFQYSISMTTRKM
                     REGEVEGVDYFFRSKEEFESMIEAGEMLEYAEYVGNYYGTPLSYINKTLDEGKDVFLE
                     IEVQGAKQVKEKVPDGVFIFLTPPDLAELKSRIVGRGTDADEVIEERMKVAKEEIEMM
                     ALYDYAVVNDEVPLAVERIKNIIASEHFRVDRVIGKYIKMLKEM"
     gene            951..1256
                     /locus_tag="IXT20_001894"
     CDS             951..1256
                     /locus_tag="IXT20_001894"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HAQ4860332.1"
                     /translation="MMLKPSIDSLLDRVNSKYSLVILASKRAHELDASAQPTLDSFDS
                     VKSVGQALEEIDAGNVVNDPHPELKRERLKMEEEERQAQKEREQHELESRIREEQKM"
     gene            1509..3917
                     /gene="priA"
                     /locus_tag="IXT20_001895"
     CDS             1509..3917
                     /gene="priA"
                     /locus_tag="IXT20_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HAQ4860333.1"
                     /translation="MTLTAEVIVDVPTMQTDQPFTYLVPSEMETALQVGMRVEVPFGN
                     GNRHVQGFVMAIQKSEESANPSLKAVIRLLDLAPVVNEELLSLADYMKKITYAFKITC
                     LQTMLPSVMKAEYDKLIYPLADTPEVQVLFDQREVISWKEAEEEGSLSQLIRWRQEQL
                     VDIKYEVHTRNKVKTIRLVRSLLTEKQIEEEWAKLRQNAKKQKELLLCLSELSQEEPI
                     AYFKNKGISTAVLNQGKEKGWLEFIESERYRDPYKDRVFDQTTALELNAEQKNAVEQI
                     ITAGQQQKDQVFLLEGITGSGKTEVYLQAIADVLSENKTAIMLVPEIALTPQMVERFK
                     GRFGESVAVLHSGLSQGEKYDEWRKIEREEAQVVVGARSAIFAPLKNIGLIIIDEEHE
                     SSYKQDETPRYHARDLAIWRSKYHHCPIVLGSATPSLESRARAQKGVYQLLQLNHRAK
                     AAAQLPAIEIVDMREEFQNHRTSTFSANLQEKIQNRLDKKEQTVLLLNRRGYSSFVMC
                     RDCGFVLPCPNCDISLTLHMDTRSMRCHYCGHEEPIPNRCPNCGGNKIRYYGTGTQKV
                     EEELRELYPQARILRMDVDTTRRKGAHEQILQKFGAKEADILLGTQMIAKGLDFPEVT
                     LVGVLNADTSLNLPDFRSSEHTFQLLTQVSGRAGRAEKAGEVVIQTFNPQHYAIELAK
                     KQNYEQFYQQEMHVRHRGGYPPYYFTVKITASHPEEQVAAKKIFQIANQLKEVLSPQS
                     LLLGPTPSMILRVKNRYYYQLIIKYKHEPNLPHVLDEILNGSQKEQRQGLFVAIDNEP
                     LNFI"
     gene            3932..4423
                     /gene="def"
                     /locus_tag="IXT20_001896"
     CDS             3932..4423
                     /gene="def"
                     /locus_tag="IXT20_001896"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HAQ4860334.1"
                     /translation="MRYPILIHPNDKLKRTAQPIDVITDETIALLDNLYETMIANDGI
                     GIAAPQVGQNKRIAVIEVDEGEKFELINPEIIEAKGESLDVEGCLSIPHVYGTVKRAD
                     EVTVRYYDRDGEEIEVTAFGYLARAFQHEIDHLDGILFIEKMIQQIPEEELEEYMEEH
                     LDD"
     gene            4416..5354
                     /locus_tag="IXT20_001897"
     CDS             4416..5354
                     /locus_tag="IXT20_001897"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HAQ4860335.1"
                     /translation="MTKLVFMGTPAFSVPILEGLIESGYEIQAVVTQPDRPVGRKRVI
                     TPTPVKEAALKHGIRVLQPEKISGSPEMEEIIELVPDVIVTAAFGQFLPEKLLQVPKL
                     GAINVHASLLPKYRGGAPVHYAIMNGEKETGVTIMEMIKKMDAGGIYAQESIPITKQD
                     DVGTMFEKLSLLGRKLLLETLPNILDGQKPVPQDESEATFSPNITREQEAIDWHKTAE
                     EIDNQVRGMRPWPIAFTTYEGIRWKLLSVEVLAETTDKAPGTIIRKDKKNLWIACGKQ
                     TVLAINELQPAGKGKQAVHDFLNGSGQQVQIGQQVK"
     gene            5354..6712
                     /gene="rsmB"
                     /locus_tag="IXT20_001898"
     CDS             5354..6712
                     /gene="rsmB"
                     /locus_tag="IXT20_001898"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HAQ4860336.1"
                     /translation="MKKIPKKMKKSVRYMALLTLERVNKGGAYSNLLLNEMIKQSELN
                     QKDVGLFTELIYGTISHQRLLEFYVAPLIAKAKKVDDWVKTLLYLSVYQLEFLDKVPA
                     HAILNEAVEIAKVKGNPGTGKFVNGVLRNYQRQGAPDLQGIADPIERLSVKISLPLWM
                     TKRLVDQIGYEETEKLGLSLYQPSHASARVDTRRLSRDQAITVLQEEEITAEKSQVSP
                     YGIVAKKGHLASSSLFHDGVMTIQDESSMLVAPSMQIEPEHHVLDACAAPGGKTTHIA
                     SFLDPSCGGEVVALDVHEHKVKLIEDNAARLGVSSAVYAQKMDAREVSKQFEAEQFDR
                     ILVDAPCSGLGLLRRKPDIRYKKQPDELANLPKIQLEILESCAPTLKSSGILTYSTCT
                     ILKEENQDVVSAFLERHPDFERIDVLTNPMLQSSIEEKMLTLYPHQFYTDGFFICCLR
                     KK"
     gene            6725..7465
                     /locus_tag="IXT20_001899"
     CDS             6725..7465
                     /locus_tag="IXT20_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HAQ4860337.1"
                     /translation="MQIEYQSDVGRRRNTNQDYASVFTSRSGLKLAVLADGMGGHRAG
                     DIASQMAVTNLGEAWEKQEAVDDEKIAQWFIQSIQEENMLIYQRGQEQPEYNGMGTTI
                     VAAALSDDWFTIANVGDSRAYLIREDKIMQLTEDHSLVNELVKSGEISEEMAVNHPRK
                     NILTRSVGMPGTVEVDVSTHLWQLKDRLLLCSDGLTNMLSEEEIGTIINQEGPLSHKV
                     TELIDRANEAGGADNITVLLIEYKEDSV"
     gene            7462..9531
                     /gene="pknB"
                     /locus_tag="IXT20_001900"
     CDS             7462..9531
                     /gene="pknB"
                     /locus_tag="IXT20_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HAQ4860338.1"
                     /translation="MIELGKKLNGRYQITGNIGSGGMANVFLAHDLILDRDVAVKVLR
                     FDFQNDQTAIRRFQREALAATELVHPNIVSVYDVGEEDNMQYLVMEYVKGMDLKRYIQ
                     THYPVPYETAVNIMQQILSAISLAHNHQIIHRDLKPQNVLIDNEGVVKITDFGIAIAL
                     SETSITQTNTMLGSVHYLSPEQARGSMATKQSDIYALGIILYEMLTGSVPFDGESAVT
                     IALKHFQDDLPSIKALDPNVPQALENVILRATAKEPADRYKSAEEMSDDLSTVLSPAR
                     ANEEKWQPHVMDNETKVITPLTEDAPMPDSFKSMPLPKDKPENETLPEEEEVLPDPKK
                     KGQKKWWLILLAILAVLGIGTAVFFASGGRGEVVVPDVSDLSEASARETLAKAKLEVA
                     TKTEEIADDKIEEGNVVKTDPAAGTTVKQKREVTLYISSGTKKIKLDDYSGKSYEDAE
                     ASLKELGFSADLIQKEEEFSSEVESGMIISQSPSEDTEVDPKTDTITFTVSKGPETVT
                     LSSHVGENYETVRNWLVTQGFNINNISATYDYSDTVPSGSIISQDPASGEVVAEETYI
                     NFVVSQGTEPKLNDISGYTKSEAQSYLASVGAEYIGHETSEYSNTVDKDKVIRTVPGA
                     GTTITKGMVVNVIYSKGPDPASSSSEKDSSDSSSSSSSSSSTSNSSKEESSSSTSSSA
                     STSESSK"
     gene            9696..10595
                     /gene="rsgA"
                     /locus_tag="IXT20_001901"
     CDS             9696..10595
                     /gene="rsgA"
                     /locus_tag="IXT20_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HAQ4860339.1"
                     /translation="MALIKGQIRKAISGFYYVYANGETYQTRGRGNFRNRKITPLVGD
                     QVIFESENQTDGYLLEILPRKNQLVRPPVTNVDLGIIVTSIVEPAFSYNLLDRFLVTL
                     EYESIEPIIFLTKTDLSDDPEKIEEIKQTYTKIGYPVIVPNHTGDTTELIRYFPERLS
                     VFMGQSGAGKSTLLNQISPELNLATGEISDALGRGRHTTRHVELIPIYGGLVADTPGF
                     SSIDFLTIEAGELPRQFPEFVEASVHCKFRECMHAKEPGCEVKHRVETGGIAQTRYDN
                     YLQFLQEIEKRKPMYAKKEGKRK"
     gene            10608..11258
                     /locus_tag="IXT20_001902"
     CDS             10608..11258
                     /locus_tag="IXT20_001902"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HAQ4860340.1"
                     /translation="MKIAPSILSADFANLQRDIELVEKGGADYIHVDVMDGQFVPNIT
                     LGPNIVQAIRPVTKLPLDVHLMIVDPENYIPAFAKAGADIITVHVEATPHIHRALQMT
                     KSLGVKAGVVINPGTPVSMIKHVLPLADQVLVMTVNPGFGGQSFIEETVEKITELADL
                     RRENNWTYAIEVDGGIVPETAKICKDAGADVFVAGSYIYGAENPVGQIERLKEALD"
     gene            11259..11897
                     /locus_tag="IXT20_001903"
     CDS             11259..11897
                     /locus_tag="IXT20_001903"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HAQ4860341.1"
                     /translation="MKILLAAGGNPQNWPQSLLSKYDAYVGIDRGALYLLQAGYPVDL
                     AVGDFDSLSNEEQKEVFAKAKEYTRSPAEKDDTDTQLGILKTFERYPQAEVTLIGATG
                     GRLDHLLANLWLGMEPRFKPFAHQLIIRDCQNYLSYYLPGEHIVRQIPDMKYLAYCCL
                     TPVSNLTLKGSKYVLENKEVEYPTSYASNEFLSDTAEFSFSDGMIAVIQSKD"
     gene            12052..12906
                     /gene="mreC"
                     /locus_tag="IXT20_001904"
     CDS             12052..12906
                     /gene="mreC"
                     /locus_tag="IXT20_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HAQ4860342.1"
                     /translation="MKKFNPNKNIIITLVIVIIVVTILSVTAAQRANEGKPNFFQSIV
                     NDSVAMVDKTISAPVRWVRNGVDSVHNLFSTYAENERLKEKIDSYDDVSQQNKNYERE
                     IEALKKELELNQTLTSYEKVTANVITRSPDSWQDMLIVDKGSKDGIEANMAVMSQKGL
                     IGRVIEVNTASSKIELLSSSNESSNHFPVRVSSANGEAFGLLKNYDEKLHALVVTQLT
                     GDTDIKEGDVVQTSGLGGNSPANLPIGTVIKTKPDSYGLDREVYVKPYAEMYDVSVVT
                     IVQRLAED"
     gene            12910..13419
                     /gene="mreD"
                     /locus_tag="IXT20_001905"
     CDS             12910..13419
                     /gene="mreD"
                     /locus_tag="IXT20_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HAQ4860343.1"
                     /translation="MLKKETMKYYLPIVLFFLMLIDGHLTRMLGEWSKGTYMSNAHFL
                     ILALLCCSMAFEKRYLLITTIVLGAIYDAYYIGVIGIYAVALPLIVWLMYVMKDVIHV
                     NIFTEFFSMIIFVTGYELFTMVVQLIFKLAVVNNTYFITRFLGPTLLLNMIIFVLFIF
                     PFKKLFSDE"
     gene            13674..15248
                     /locus_tag="IXT20_001906"
     CDS             13674..15248
                     /locus_tag="IXT20_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4860344.1"
                     /translation="MKKSLISAVMVCSMTLTAVASPIAAAADDFDSQIQQQDQKIADL
                     KNQQADAQSQIDALESQVSEINTQAQDLLAKQDTLRQESAQLVKDIADLQERIEKRED
                     TIQKQAREAQVSNTSSNYIDAVLNADSLADAIGRVQAMTTMVKANNDLMEQQKQDKKA
                     VEDKKAENDAKLKELAENQAALESQKGDLLSKQADLNVLKTSLAAEQATAEDKKADLN
                     RQKAEAEAEQARIREQQRLAEQARQQAAQEKAEKEAREQAEAEAQATQASSTAQSSAT
                     EESSATQSSMTEESSSATQSSATEESTTPESSTEESTAPESSATEESTTAPESSATEE
                     STTVPESSATEESTTVPESSTTEESTTPAPTTPSTDQSVDTGNGTGSSTPAPTPTPTP
                     EQPKPVTPAPAPSGSVNGAAIVAEAYKYIGTPYVWGGKDPSGFDCSGFTRYVYMQVTG
                     RDIGGWTVPQESAGTKISVSQAKAGDLLFWGSQGGTYHVAIALGGGQYIHAPQPGESV
                     KVGSVQWFAPDFAVSM"
     gene            complement(15316..17115)
                     /locus_tag="IXT20_001907"
     CDS             complement(15316..17115)
                     /locus_tag="IXT20_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HAQ4860345.1"
                     /translation="MSYSLNWNLDSIFSGGSHSDALNQRMKQLEDQMNEYYHRVTKWS
                     PSSDNAEQLNAILQLQETITNGFTQCSSYITALLSANVNDSDAKVLSGKLYAMLPRLQ
                     SAETVLSKKFAEISDNDWNQMLSQSSFETIAFRLNEIRRDGSQLLSEAEENIINTLSL
                     DGLNAWSSHYDTIVASISIPFEQDGQVVELSAGQAFNKMMGDPDPKVRETLFAAWEEA
                     WKEKAPLFTDTLNHLDGFRLSDYELHGVTDFLQKPLEYNRLKKETLSVMWDTIQKNKQ
                     PLVNYLTRKANLFGKEKMEWQDQDAPIILGDLKEKTFSFDEAAAFIIENFNKFSPKMA
                     KFAQSAFEKSWIEAEDRPGKRPGGYCTELPETKESRIFMTYSNSVNEVATLAHELGHA
                     FHSSTMWDLPSLNREYAMNVAETASTFAELIVADATLKVAKTKEEKINLLDTKLQNAL
                     AMFMNIHSRFIFENRFYEARQEGLVSEDKITEMMVEAQKEGYQGALATYHPYFWAAKL
                     HFFIDDVPFYNFPYTFGYLFSLGIYAYANQKGANFEEEYIHLLRDTASMTTEDLAKKH
                     LNVDLTQPDFWQAGIDMVTEDIQTFMELTEEFI"
     gene            17238..17744
                     /locus_tag="IXT20_001908"
     CDS             17238..17744
                     /locus_tag="IXT20_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HAQ4860346.1"
                     /translation="MNSSKKQMKNGNIYLVIALIVVIILFYSSSQTYGQQSQVSRLES
                     WLPNQPFKDTLAGIRFNYGGSMVSIDHLGYAKFLEFFLRKGAHFGTYFILGGSLFLGV
                     FPKLKIWWLTAILAWLSATGYAGLDEFHQMMTGGRTPMFQDVMLDSIGALTAVILCVL
                     FAAFKKRL"
     gene            17823..18545
                     /locus_tag="IXT20_001909"
     CDS             17823..18545
                     /locus_tag="IXT20_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAQ4860347.1"
                     /translation="MSGHSKWHNIQGRKNAQDAKRGKVFQKLSREIYMAAKAGGPDPS
                     MNPALRLVVDKAKAANMPNDNVERAIKKATSAVDETNYDEITYEGYGPGGVGILVHAL
                     TDNRNRTATNVRVAFTRNGGSLGETGSVSYLFDRKGYIAIERDGLTVDEETMFEDVLE
                     AGAEDLQTSPEVFEIYTAPEDFAAVRDELEKEFTLAQAELTMIPQTTIDLNEEQKEQL
                     ERLVDKLEDDDDVSEVFTAAEM"
BASE COUNT         6117 a         3467 c         4017 g         5002 t
ORIGIN      
        1 tattaagcca aatttatgca aaaatgtgag aaactatttt cacaaaattt ttcttcatac
       61 tcgaaactaa tcggcttctt cacgacctct tatacacggc gttcaaaagc ttgcatctgc
      121 gataataacc ccaaccaaac gaaaaacatg agaagctgtt tatgtttggc tgttcttatt
      181 acttgatgca ggacagcttt tttacgacct cttggtacag ggtagctttt ttaccaattt
      241 ctagctataa ttaaagataa ccttgaaagt tgcagaaaaa aagtgcaaaa taaacgataa
      301 gtacaaaaag actagttgaa aggacgtcgt catgtcagag cgtggattat taatcgtgtt
      361 gtctggtcca tccggtgtag gaaaaggcac agtacgaaaa gcaatttttg agagtgagga
      421 taatgatttt cagtattcga tctcaatgac tacccgtaaa atgcgtgaag gggaggtcga
      481 aggcgttgat tatttcttcc gaagcaaaga agagtttgaa tcaatgatcg aagctggaga
      541 gatgctggaa tacgcagaat atgtcgggaa ttattatgga actcctttgt catatatcaa
      601 caagacattg gatgaaggaa aagatgtttt cttggagatt gaagtccaag gagcaaaaca
      661 agtcaaagaa aaagtgcccg atggtgtatt catctttttg actccgccag atttagcgga
      721 attaaaatca cggatcgtag gccgaggaac agatgcagat gaagtaatcg aagaacgaat
      781 gaaagttgca aaagaagaaa tcgaaatgat ggctctttac gactatgctg ttgtaaatga
      841 tgaagtgccg ctagcagttg aacgaatcaa aaacattatt gccagtgaac attttcgagt
      901 agatcgcgta atcggtaaat atattaaaat gttaaaggag atgtagtctt atgatgttaa
      961 aaccttcaat tgactcacta ttagatcgtg taaattcaaa atattctcta gtgattttag
     1021 caagtaaacg cgcgcatgaa ttggatgcaa gtgcacagcc tactctagat tcttttgatt
     1081 cagtaaaaag cgtcggtcaa gctttagaag aaatcgatgc aggaaatgta gtcaatgatc
     1141 cacatcctga attaaaacgt gaacgtttga agatggaaga agaggaacgt caagcacaaa
     1201 aagagcgtga acaacacgaa ttggaatcac gtatccgtga agagcaaaaa atgtaataaa
     1261 gaaaaataga gcgaacatct aaaaagaaaa gcaaacccag ctgtttcctt ttcaaacatt
     1321 cgccacttat tgtctggtag aaaaggctgt gtctgaactt tttgacacag ttcttttttt
     1381 tcttatctgc tgttaatttt ttgttaggat ttggcagaag ataagacaaa tccatcgatc
     1441 tctagtaaaa taggcaagga actttttgtc gcttgataag aaaagacaaa aaaaggagga
     1501 aacgaaccat gacattaaca gctgaagtca ttgtggacgt acctactatg cagaccgatc
     1561 agccattcac ttatttggtt ccctctgaaa tggaaacagc tttgcaagtc gggatgcgag
     1621 tagaagtccc ttttggtaat ggcaatcgtc atgtccaagg atttgttatg gctatccaaa
     1681 aatcagaaga atcagctaat ccatctttga aggcagttat tcgtttgcta gaccttgctc
     1741 cggtggtcaa tgaagaatta ctttcattag ctgattatat gaaaaaaatt acttacgcat
     1801 tcaaaatcac ttgtctacaa acgatgcttc cgagtgtgat gaaagcagaa tatgataaat
     1861 tgatctatcc tttggcagac acaccagaag ttcaggtatt gtttgatcag cgagaagtga
     1921 tttcctggaa ggaagcagaa gaagaaggca gcctttctca attgattcgc tggcgccaag
     1981 agcaattagt agatatcaaa tatgaagtcc atacacgaaa taaagtaaaa acgattcgtt
     2041 tggtccgttc tttgttaact gaaaagcaaa tcgaagaaga atgggcaaaa ttacggcaaa
     2101 atgccaagaa acaaaaagaa cttcttttgt gtttatctga actgagccaa gaagaaccaa
     2161 ttgcttattt caaaaataaa ggaatcagta ctgctgtctt gaatcaagga aaagaaaagg
     2221 gctggctgga gtttatagaa tcagaacgtt atcgcgaccc ttataaagac cgagtatttg
     2281 accaaacgac ggcattggaa ctgaatgcag aacaaaaaaa tgctgtcgaa caaatcatca
     2341 cagcaggtca gcagcaaaaa gatcaggtct ttttgctgga gggaatcact ggcagcggga
     2401 aaacagaagt atatcttcag gcaatcgctg atgttttgtc agaaaataaa acggctatta
     2461 tgttagtgcc tgaaatcgca ttgactccgc aaatggtcga gcgattcaaa ggacgcttcg
     2521 gagaatcagt tgcggtctta catagcggac tttcccaagg tgaaaaatat gatgaatggc
     2581 gcaagattga gcgggaagaa gcgcaagtag tagtaggcgc acgatctgct atttttgccc
     2641 cgctgaaaaa tatcgggctg atcattatcg atgaagaaca tgaatcaagt tacaaacaag
     2701 atgagacacc gcgttatcat gcgagagatc tagcaatttg gcgcagtaaa tatcatcatt
     2761 gcccaatagt tctaggaagc gcgacgccct ctttagaatc aagagcacga gctcaaaaag
     2821 gcgtttatca gttattgcag ttaaaccacc gagccaaagc agctgcacag cttccagcta
     2881 ttgagatcgt agatatgcgt gaagagtttc agaatcatcg gacctctact ttttctgcta
     2941 atttgcagga aaaaatacaa aatcgattag acaaaaaaga acaaactgtc ctactgctca
     3001 atcgtcgagg atattcgtct tttgtgatgt gtagggactg tggatttgtt cttccatgtc
     3061 ctaattgcga tatctctctt acactgcata tggatacacg ttcaatgcgc tgccattatt
     3121 gcggacatga agaacctatt cccaatcgct gtcctaattg tggcggcaat aaaatccgct
     3181 actatggaac aggtactcaa aaagtagaag aggaactccg agaactttat ccgcaagcta
     3241 ggattttgcg tatggatgta gataccactc gaagaaaagg ggcacacgaa cagattttac
     3301 aaaaattcgg tgcaaaagaa gcagatatat tattagggac acaaatgatt gctaaaggtt
     3361 tggactttcc agaagtgaca ttagttgggg tgttgaatgc tgatacctcc ctgaatttac
     3421 cggattttcg ttctagtgag catacattcc aattgcttac ccaagtttca ggacgagcag
     3481 ggcgagcgga aaaagcaggc gaagtcgtga tccaaacatt caatcctcaa cattacgcca
     3541 tagaattagc taaaaaacaa aattatgagc agttttatca gcaggaaatg catgtacggc
     3601 atcgtggagg ctatcctcct tattacttta ctgtgaaaat aaccgccagc catcctgaag
     3661 aacaagtagc ggcaaagaaa atctttcaga ttgccaatca gctaaaagaa gtgttaagcc
     3721 ctcaaagctt gctattaggg ccaacgccca gcatgatttt gagagtgaaa aaccgatatt
     3781 attatcaact aatcatcaaa tataaacatg aaccgaatct tcctcatgtt ttggatgaaa
     3841 ttttgaatgg aagccagaaa gaacagcgac aaggtctttt tgtggcaatt gacaacgaac
     3901 cattaaactt tatttaagaa agggccaaat tatgcgctat cctatattga tccaccctaa
     3961 tgataaatta aaaagaactg ctcagccaat cgatgtaatc acggacgaga caatcgcttt
     4021 attagacaat ttatatgaga caatgattgc gaatgacgga atagggatcg ctgctcctca
     4081 agtaggacaa aacaagagaa ttgctgtgat cgaagtagat gaaggcgaga aatttgaact
     4141 gattaaccca gaaatcatcg aagcaaaagg tgaaagcctt gacgtagaag gttgtctcag
     4201 tattccgcat gtctatggaa cggtcaagcg agcagatgaa gtgaccgtgc gttattatga
     4261 tcgagacgga gaagagatag aagtgacggc ttttggctat ttagcccgtg ccttccagca
     4321 tgaaatcgat cacttagacg gtatcttgtt cattgagaaa atgatacagc agatcccgga
     4381 agaagaactt gaagaatata tggaggaaca tcttgatgac taagctagta tttatgggca
     4441 cacctgcttt ttctgtgccg attcttgaag ggctgataga atcaggttac gagatccagg
     4501 cagtggtaac acagcccgac cgtccagttg ggcggaaacg agtaattacg ccgacaccgg
     4561 taaaagaagc ggctctgaag cacggtatcc gtgtgctgca gccagagaaa atctccggtt
     4621 cgccagaaat ggaagaaatc attgaattgg tgcctgatgt gatcgtcact gcggcatttg
     4681 gccaattctt gcctgaaaag ttattgcaag taccaaaatt aggggcaatc aatgtccatg
     4741 cttctttact tcctaaatat cggggaggtg cgcctgttca ttatgccatc atgaatgggg
     4801 agaaagaaac tggtgtaacg atcatggaaa tgatcaaaaa aatggatgca ggcggaatct
     4861 atgcgcaaga aagcatacca atcactaaac aagatgatgt agggacgatg tttgaaaagc
     4921 ttagcctttt aggaagaaag ctattgctag aaacattgcc caatattttg gatggacaaa
     4981 aaccagtacc tcaagacgag tcagaagcaa ctttttcgcc aaacatcaca cgggaacaag
     5041 aagcaatcga ttggcataaa actgcagaag aaattgacaa ccaagtacgc ggaatgcgtc
     5101 cttggccaat tgcattcacg acctatgagg gcatacgctg gaaattatta tcggttgaag
     5161 tattggcaga aactacagac aaagcacctg gtacgatcat ccggaaagac aaaaaaaatc
     5221 tatggattgc ttgcggtaaa cagaccgtct tagccatcaa tgaattacag ccagctggta
     5281 aaggaaaaca ggccgtacat gatttcctta acggaagcgg tcagcaagtt cagattggtc
     5341 agcaggtgaa ataatgaaaa aaatccctaa aaaaatgaaa aaatcagtac gatatatggc
     5401 attactgaca ttagagcgag tgaacaaggg tggcgcctac tcgaatcttt tgttgaatga
     5461 gatgatcaaa caaagcgagc taaatcaaaa agatgtgggt ttatttacag aattgatcta
     5521 tggtacgatt agccatcagc gactattgga attttacgtt gcaccattga tcgctaaagc
     5581 aaaaaaagtg gatgactggg tcaagacgct tctttatctt tctgtatacc aattagaatt
     5641 tttagataaa gtccctgcac atgctatttt aaacgaggcg gtcgaaatcg caaaagtaaa
     5701 aggaaatcct gggacaggga aatttgtaaa tggtgtcttg aggaattatc agcgtcaagg
     5761 agcaccagat ctgcaaggca ttgctgatcc tatcgaaaga ctttctgtca aaatcagcct
     5821 gcctctatgg atgacgaaac gactggtgga tcaaatcgga tacgaagaaa ctgaaaagct
     5881 cggtttatcg ctatatcagc ctagccatgc tagtgcccga gtagatacac gccgactttc
     5941 cagagatcag gccatcactg ttttgcaaga agaagagatc acagctgaaa aaagccaagt
     6001 cagtccatac ggtatagtag cgaaaaaagg gcatttagct tctagctcat tgttccacga
     6061 tggcgtcatg acgatccaag atgaaagttc gatgcttgta gcgccgtcta tgcaaatcga
     6121 gcctgaacat catgtcttag atgcctgtgc agcacccgga ggtaagacga cgcatattgc
     6181 ttctttttta gatccgtcat gcggtggaga agtggtcgct ttagatgtac acgaacacaa
     6241 agtcaaactc attgaagata atgcagcacg tctaggtgtc tcttctgctg tctacgctca
     6301 aaagatggat gctagagaag tcagtaaaca gttcgaagca gagcagtttg atcggatttt
     6361 agtagacgcg ccttgttcag gtcttgggct gcttcggcga aaaccagata ttcgttataa
     6421 aaaacagccg gatgaattag cgaatttacc aaaaatccaa ttggaaattt tagaaagctg
     6481 cgcaccaact ttaaagtctt ccggtattct gacttatagc acttgtacga tcttaaaaga
     6541 agaaaaccaa gacgttgttt ctgctttctt ggaaagacac cctgattttg agaggattga
     6601 tgttttgacc aatcctatgc tacaatcatc aatcgaagag aaaatgttga ctttgtatcc
     6661 gcatcagttt tacacagatg gatttttcat ttgttgtttg cgcaaaaagt aatgaggtga
     6721 aaaaatgcag attgaatatc aatcagatgt aggaagacga cgtaacacaa atcaagacta
     6781 tgccagtgta tttaccagcc gttcaggtct caagcttgct gtattagcag acgggatggg
     6841 tggtcatcgt gccggagaca ttgccagtca gatggcggta acgaatctag gcgaagcttg
     6901 ggaaaaacaa gaagctgttg acgatgaaaa aatcgcccaa tggttcattc aatcgattca
     6961 agaagaaaat atgctgattt atcagcgtgg acaagaacag ccggaatata acggtatggg
     7021 aacaacaatc gtagcggccg ctctgtcaga tgactggttc acgattgcca atgttgggga
     7081 tagcagagct tatttgattc gtgaagataa aatcatgcaa ttgacggagg atcattcttt
     7141 agtcaatgaa ctagtcaaat caggagaaat cagcgaagaa atggcagtga accatccaag
     7201 gaaaaatatt ttaacacgtt ctgtcggcat gcctggaaca gtcgaagtag atgtttctac
     7261 tcatctttgg caactaaaag atcgactgct gttatgttca gatggcttga cgaacatgct
     7321 gtctgaagaa gaaataggaa cgatcatcaa tcaagaaggc ccattgtctc ataaagtaac
     7381 tgagctgatt gatcgtgcca atgaagcagg cggagcggat aatatcaccg ttcttcttat
     7441 cgaatataag gaggacagtg tatgatcgag ttgggcaaaa aattaaatgg ccgttatcaa
     7501 atcactggaa atatcggtag cggtggtatg gcaaatgtat ttttggcgca cgatttaatc
     7561 ttggatcgtg atgtagcagt taaagtctta cgatttgact ttcaaaatga ccagactgcg
     7621 attcgacgat ttcagagaga agctttagca gcaacagaac tcgttcatcc aaacattgtt
     7681 agtgtttacg atgttggtga agaagacaac atgcagtatc tggtgatgga atacgtcaaa
     7741 ggaatggact tgaaacgata tatccaaaca cattatcctg ttccttatga aacagcagta
     7801 aatattatgc agcagatcct ttctgctatt tctttagctc ataaccatca aatcatccac
     7861 cgagatttga aaccccaaaa cgtactgatc gataatgaag gtgttgtaaa aatcacggat
     7921 ttcgggattg ctatcgcttt atctgaaaca tcgatcacgc agacgaatac gatgctggga
     7981 tctgttcatt atctgtcccc tgagcaggcc agaggaagta tggcaacgaa gcagtcagat
     8041 atttatgctt tagggatcat cctttatgag atgttgacgg gtagcgtacc gtttgatggt
     8101 gaatctgctg taacaattgc tttaaaacat tttcaagatg atcttccatc tataaaagca
     8161 cttgatccaa atgtgccaca agcactggaa aacgtgattt tacgggcaac ggctaaagaa
     8221 cctgcagacc gttataagtc agccgaggaa atgtctgatg atctttctac agtattatca
     8281 ccagctcgtg caaacgaaga aaaatggcag cctcatgtga tggataatga aacaaaagtt
     8341 atcacacccc ttacggaaga tgcaccaatg cctgattcat tcaaatctat gcctctgcca
     8401 aaagacaagc cagaaaacga aaccttgcca gaggaggaag aagttctgcc tgatcctaaa
     8461 aagaaaggac agaaaaaatg gtggctgatc ctattagcta ttcttgctgt tttaggaatc
     8521 ggaacagctg tattctttgc ttctggcggg cgaggagaag ttgttgttcc tgatgtttct
     8581 gatctttctg aagcttctgc tagagaaaca ctcgcaaaag cgaaattaga agtagcaaca
     8641 aaaacagaag aaattgcaga tgacaaaatc gaagaaggta atgtagtgaa gacagacccg
     8701 gcagcgggaa cgacagtcaa gcaaaaacgc gaagtcactc tgtatattag tagcggcacg
     8761 aaaaaaatca aattagatga ttacagtggt aaaagttatg aagatgccga agcaagtttg
     8821 aaagaattag ggttttctgc tgacttgatt caaaaagaag aggaattcag ttcagaggta
     8881 gaaagcggaa tgatcatttc gcaatctcca tcagaagata cagaagtcga tcctaaaaca
     8941 gacacgatta ctttcacagt cagcaaagga ccagaaacag tgactttgag tagtcatgta
     9001 ggagaaaact acgaaactgt tcgtaattgg cttgttacac aaggattcaa tatcaataat
     9061 attagtgcta cttatgacta tagcgacact gtaccatcag gttcgatcat cagtcaggat
     9121 ccagcctcgg gtgaagttgt agcagaagaa acttacatca attttgtcgt aagccaagga
     9181 acagagccta aattgaatga tatctccggc tatacaaaaa gcgaagcaca aagttattta
     9241 gctagtgtcg gtgcagaata tatcgggcat gagacttccg aatattcaaa cacggtggat
     9301 aaagataaag tgattcgtac agtaccaggg gcaggaacga caattaccaa aggaatggtt
     9361 gtgaacgtaa tttactctaa aggaccagat ccagcgtctt ctagttctga aaaagactct
     9421 tcagacagtt ctagcagctc aagtagctcc agtagcacaa gcaactcttc aaaagaagaa
     9481 tcttccagct ccacaagttc atcagctagc acctcagaaa gttcaaaata agtgctgaat
     9541 aaaatatatc agaagggaat gcgacattga tttgttgcat tctctttttt gtgtttctat
     9601 ctcttttgat caattttcaa gccatatact ttgagaagtt tgcatggaat aacacctact
     9661 ttcctcttcg tatggtaaaa taaacaaaaa cggaggtggc acttatcaaa ggacaaatca
     9721 gaaaagcaat cagcggtttt tactatgtat acgcaaacgg agagacctat caaacaagag
     9781 gaagaggaaa tttccggaat cggaaaatca cgccgcttgt cggagatcag gtgatctttg
     9841 agagtgaaaa tcaaacagac ggctatcttc tggaaatctt gccgagaaaa aatcaattag
     9901 ttcgcccacc agtcacaaat gttgatcttg gaatcattgt cacaagtatc gtagagcctg
     9961 cattttctta taatctactt gaccgctttc tagttacctt ggaatacgag tcgatcgaac
    10021 cgattatttt tttgacaaag accgacttgt cagacgaccc agaaaaaatc gaagagatca
    10081 agcaaaccta tactaaaata ggctatccgg tgattgtacc caatcatacg ggtgatacga
    10141 cagaactgat ccgttacttc ccagaacggc tatcggtctt tatgggtcag tctggagcag
    10201 gaaaatcgac gctactgaat caaatatcac cggaactgaa tttagcaaca ggagagattt
    10261 ctgatgcact tggaagagga cggcatacaa cacgtcatgt ggaactgatt cctatctatg
    10321 gcgggttagt agcagataca ccaggattta gctcgattga ctttttgaca attgaagcag
    10381 gagaactgcc gagacaattt cctgaatttg tagaagcttc tgtccattgc aaatttagag
    10441 agtgcatgca tgctaaagaa cctggctgcg aagtaaaaca tagagtagaa acaggtggta
    10501 tcgcacagac acgttacgac aattatttgc aattcttaca agaaatcgaa aaaagaaaac
    10561 caatgtatgc aaaaaaagaa ggtaaacgaa aataggagga aacaagaatg aagatagcac
    10621 catccatttt gagtgcagat tttgccaatt tacaaagaga cattgagtta gtggaaaaag
    10681 gaggcgcaga ttacatccac gtagatgtaa tggatggcca atttgtccca aatatcacat
    10741 tagggccaaa tattgttcag gcaatccgtc ctgttactaa attaccgtta gacgtccatc
    10801 tgatgatcgt tgatccagaa aactacattc cagcatttgc aaaagctgga gcagatatca
    10861 ttactgtcca cgtcgaagca actcctcata ttcatcgggc acttcaaatg acaaaaagct
    10921 taggagttaa agctggagta gttatcaatc caggaacacc agtctccatg atcaaacatg
    10981 ttttgccttt ggctgatcaa gtactagtca tgactgtcaa tcctgggttc ggcggacaaa
    11041 gctttattga agaaacagta gaaaaaatta cggaactcgc tgatctacgt agagagaaca
    11101 attggactta tgcaatcgaa gtagatggcg gaatcgttcc agaaacagca aaaatctgta
    11161 aagatgcagg ggcggatgtc ttcgttgcag gatcatatat ctatggagcc gaaaatccag
    11221 tcggacaaat cgaacgatta aaagaggcat tagactaaat gaaaatttta ttagctgctg
    11281 gaggtaatcc gcaaaactgg ccgcagtctc tgctgtccaa atacgatgcc tatgtcggga
    11341 tagaccgtgg tgcgctgtac ctgcttcaag caggctaccc agtagatctg gcagtgggcg
    11401 attttgattc attgtcgaac gaagagcaga aggaagtatt tgcaaaagcc aaagagtata
    11461 cccgttcccc tgcagaaaaa gacgatacag atacacagtt aggtatctta aaaacatttg
    11521 aacgttaccc gcaagcggaa gtcactttga ttggagctac gggcggaaga ttggatcatc
    11581 ttttagccaa tctctggtta ggtatggaac ctcgcttcaa accctttgcc catcagctta
    11641 ttatcagaga ttgtcaaaat tatttgtctt attatttgcc tggagagcat atagtaagac
    11701 agataccaga tatgaagtat ttagcttatt gctgcttgac tcccgtctcg aatcttacat
    11761 taaaaggcag caagtatgta ttggaaaata aagaagtaga atatccgaca tcttatgcaa
    11821 gtaacgaatt tctttcagat accgcagaat tttctttttc agatggtatg attgcagtta
    11881 ttcaaagcaa agattgatcc ataaacaaat aaaaaaattt gtctttcagg ctaaaaatga
    11941 cagaatctat tatttgctaa aaaaaagctt aagaggagca acttaccttt tgtttcacgg
    12001 catttagtga taaaattgaa cacaagagtc taattaattc gaggtgtctt tgtgaagaag
    12061 ttcaatccta ataaaaatat tattattacc ttggttattg ttatcatcgt ggtgacgata
    12121 ctaagtgtga ccgctgcgca gcgagcgaat gaaggaaagc ccaatttctt ccaatccatc
    12181 gttaatgaca gtgttgcaat ggttgataaa acaatctctg ccccagtacg gtgggtacga
    12241 aatggagtag attctgtcca taatcttttt tcaacttatg ccgaaaatga gcggctaaaa
    12301 gaaaaaatcg atagctatga tgacgtttca cagcaaaaca agaattacga gagagaaatc
    12361 gaagcgttga aaaaagaact tgagttgaat cagacgctga ctagttacga aaaagtaacg
    12421 gcaaatgtca ttactcgttc gcctgacagt tggcaggaca tgctgatcgt agacaagggt
    12481 tctaaagacg gtatcgaagc aaatatggca gtcatgtcgc aaaaaggatt gattggccga
    12541 gtgatcgagg tcaatacggc ttcgtctaaa atcgaattac tgtcatcctc taatgaaagc
    12601 tccaatcatt ttccagtacg ggtatcttcg gctaatggcg aagcgtttgg tttgcttaaa
    12661 aactatgatg aaaagctcca tgccttagtg gtgacccaat taactggtga tacggatatc
    12721 aaagaagggg atgttgtcca gacatccggt cttggaggga attctccagc taacttgccg
    12781 atcggtacgg ttattaaaac gaaaccagat agttatgggc tggatcggga agtttatgtg
    12841 aaaccttatg cagaaatgta tgacgtgtca gttgtgacga ttgtccaacg gctagcggag
    12901 gattagtcca tgttgaagaa agaaacaatg aagtactatc tgccaatcgt tttgttcttt
    12961 ttgatgttga tagatggtca tttaacaaga atgctagggg agtggtcgaa aggcacctat
    13021 atgtcaaatg cccactttct gatattggca ttattatgtt gcagtatggc gtttgaaaaa
    13081 cgttatttac tgattaccac gattgttctc ggggctatct atgacgctta ctatattggc
    13141 gttatcggta tctatgcagt agctctccct ttaattgtat ggttgatgta tgtaatgaaa
    13201 gacgttatcc atgtcaacat ctttactgaa tttttcagta tgatcatctt tgtcacgggt
    13261 tatgaattgt ttacgatggt ggtccagttg atttttaaat tagcagtagt aaataacacg
    13321 tattttatta caaggttttt aggacctaca ctgctgttga acatgattat atttgtatta
    13381 ttcatttttc cctttaagaa attattcagt gatgaataat ttcttttttt atttttaggt
    13441 cgaaagcgaa aaagaatcgt tgttatctta agaaatgcta gacttaacgc ggctttttca
    13501 tgatcatttt ctgaaacgaa cagttttgtc atttttaaaa gttgttttga aacatttttg
    13561 taacataact attatatcgt tgacacactg ctatggtaca atttttatat cgttaagaaa
    13621 gacttaaata tatttagata tttattaaat taagccagtc ggaggaatga agagtgaaaa
    13681 agagtttaat atcagcagta atggtatgtt caatgacctt gactgccgta gcatcaccga
    13741 tcgctgccgc ggcggacgat tttgattctc agatacaaca gcaagatcaa aagattgcag
    13801 acttgaaaaa tcaacaagca gatgcacaat cacaaatcga tgccttagaa agccaagttt
    13861 ctgaaatcaa cacacaagca caagacctat tagcaaaaca agatacattg cgtcaagaat
    13921 ctgcacaatt agtaaaagac attgcagatt tacaagaacg tattgaaaaa cgtgaagata
    13981 caatccaaaa acaagcacgc gaagcacaag taagcaacac aagctcaaac tacatcgatg
    14041 ctgtattgaa tgctgattca ttagcagatg ctattggacg cgttcaagca atgacaacga
    14101 tggtaaaagc aaacaacgac ttgatggaac aacaaaaaca agataaaaaa gctgtagaag
    14161 acaaaaaagc tgaaaacgat gctaaactaa aagaattagc tgaaaaccaa gctgctttgg
    14221 aatctcaaaa aggcgatctt ttatctaaac aagctgattt gaacgtactg aaaacttcat
    14281 tagctgctga acaagcaact gctgaagaca agaaagctga cttgaaccgt caaaaagcag
    14341 aagctgaagc agagcaagct cgtattcgtg aacaacaacg tctagcagaa caagctcgtc
    14401 aacaagctgc acaagaaaaa gctgaaaaag aagcacgcga acaagcagag gctgaagcac
    14461 aagctactca agcttcatct actgcacaaa gctcagcaac agaagaatca tcagctacac
    14521 aatcttcaat gactgaagaa tcatcatcag ctacacaatc ttcagcaaca gaagaatcaa
    14581 caacacctga atcttcaaca gaagaatcaa cagcgcctga atcttcagca acagaagaat
    14641 caacaacagc gcctgaatct tcagcaacag aagaatcaac aacagtgcct gaatcttcag
    14701 caacagaaga atcaacaaca gtgcctgaat cttcaacaac agaagaatca acaacacctg
    14761 cgcctacaac accatcaaca gatcaaagtg ttgatacagg aaacggcaca ggaagtagta
    14821 ctccggctcc aacgccaaca ccaacacctg aacaaccaaa acctgtaaca cctgctccag
    14881 ctccttctgg aagtgtgaat ggtgcagcaa tcgtagcaga agcttacaaa tatattggta
    14941 ctccatatgt ttggggcggt aaagatccaa gtggctttga ctgctcaggg ttcacacgct
    15001 atgtttacat gcaagtaact ggtcgtgaca tcggtggttg gacagttcct caagaatcag
    15061 ctggaacaaa aatttcagtt tctcaagcaa aagctggaga cttgttattc tgggggtcac
    15121 aaggcggaac ttaccacgta gcgattgctt taggcggagg acaatatatc catgctcctc
    15181 aaccaggcga aagcgtaaaa gttggttcag ttcaatggtt tgcccctgac tttgctgtca
    15241 gcatgtaaga atgattaatc aaaaagagtt agcccattct ggactaactc tttttttatt
    15301 gtgataaaat agaaatcaga taaattcttc tgtcaattcc atgaacgttt ggatatcttc
    15361 agtcaccatg tcgatacctg cttgccaaaa atctggttgg gtaagatcga cattcaaatg
    15421 tttcttcgca agatcttccg tcgtcattga ggcagtatcg cgtagtaaat ggatatattc
    15481 ttcttcaaaa tttgctccct tttggttggc gtaagcatag atgcccaaac taaagaggta
    15541 gccaaatgtg tatgggaagt tataaaacgg tacgtcatca ataaagaaat gcaattttgc
    15601 agcccagaaa tatggatggt aagtagctaa agctccttga tagccttctt tttgcgcctc
    15661 aaccatcatc tctgtgatct tatcttctga gactaatcct tcttgccgtg cttcatagaa
    15721 tcgattttca aaaatgaatc gggaatggat attcatgaac atagctaaag cattttgcag
    15781 ttttgtatct agcaaattga ttttttcttc ttttgttttt gcgactttta atgtagcatc
    15841 ggctacgatc agttctgcga atgtacttgc tgtttctgcg acattcatgg catattcgcg
    15901 atttaaagaa ggtaaatccc acatcgtgct gctatggaaa gcatgtccta gttcatgagc
    15961 aagtgtcgca acctcattaa cagaattact gtaggtcata aagatccttg attcttttgt
    16021 ttctggtaac tcagtacaat aaccaccagg acgtttccca ggacgatctt ctgcttcgat
    16081 ccagctcttt tcaaaagcag attgggcaaa tttagccatt tttggactga atttgttaaa
    16141 gttttcgata ataaatgctg ctgcttcgtc aaaactaaag gttttttctt tcaaatcgcc
    16201 taaaataata ggtgcatctt gatcttgcca ttccattttt tctttaccga aaagattagc
    16261 ttttctagtc aagtaattaa caaggggttg tttgtttttc tggattgtat cccacatgac
    16321 agacaatgtt tctttcttca accgattgta ctctaatggt ttttgtagaa aatctgtgac
    16381 accatgaagt tcatagtcag aaagtctaaa accatctaaa tgattcaaag tatccgtgaa
    16441 gaggggtgcc ttttctttcc aggcttcttc ccatgctgcg aaaagtgttt ctctgacttt
    16501 tggatctgga tcgcccatca tcttattgaa ggcttgtcca gcagataatt cgactacttg
    16561 tccatcttgt tcgaagggaa tcgaaatgct ggctacaatc gtatcataat gactgctcca
    16621 agcatttaga ccatctaaag aaagcgtatt tataatgttt tcttcagctt ctgataataa
    16681 ttgtgagcca tcacgacgaa tctcgtttaa acgaaaagca attgtttcaa acgaagattg
    16741 agaaagcatc tgattccaat cattgtcaga tatttctgca aatttctttg acaatacagt
    16801 ttcggctgat tgcagacgag gcagcattgc gtaaagcttg cctgataaga cctttgcatc
    16861 tgaatcattg acattggcag aaagcaatgc agtaatatag ctgctgcatt gggtaaaacc
    16921 attcgtaatc gtttcttgca attgaagaat agcgtttaac tgctctgcat tgtcagaact
    16981 aggtgaccat tttgttacgc ggtgataata ctcgttcatt tggtcttcca attgcttcat
    17041 ccgttggttc aaagcatcag aatgactgcc tccggaaaaa atagaatcta gattccaatt
    17101 taatgaataa ctcatgaaaa aactcccttc atattgtaat acaaaattaa gtatagcact
    17161 tttttatgga agaagtctct atgaaaatga ttgttgtttc ttcaaaaaaa gtctaagata
    17221 aaatacagag gtgacttatg aattcttcaa aaaaacaaat gaaaaacgga aatatttatt
    17281 tagtaatcgc actgatagtc gttataatct tattttacag ttcttctcaa acttacggac
    17341 agcaatcaca agtttcgcga ttagaaagct ggctgcctaa tcagccattt aaagacacat
    17401 tagcgggtat acgttttaat tatggtggaa gtatggtgag tatcgaccat ctgggctacg
    17461 ccaaattttt agagttcttt ttaaggaagg gcgcacattt cggtacctat tttattttgg
    17521 gaggcagctt gtttttaggt gttttcccca agctaaaaat ttggtggctg acagcgatcc
    17581 ttgcttggct ttcagcaaca gggtacgcag gtttggacga attccatcag atgatgaccg
    17641 gtggacgaac acctatgttc caagatgtca tgttggattc gatcggtgct ctgactgctg
    17701 tgatattatg tgtgcttttt gcggcattca agaagcgttt atagattggc tgtggctttg
    17761 tttttttgtg aaatagtact agaatagaag tgtattctca aaagaacaag ggaggaaaaa
    17821 tcatgtcagg acatagtaaa tggcataata tccaaggaag aaaaaatgcc caagatgcaa
    17881 aacgagggaa agtctttcag aaactttctc gtgaaattta tatggcagct aaagcaggcg
    17941 gtcctgatcc atctatgaat ccagctttgc ggcttgttgt ggacaaagca aaagctgcaa
    18001 atatgcctaa cgacaatgtg gaacgggcga ttaaaaaggc tacgagtgcc gtagatgaaa
    18061 cgaattacga tgaaatcacg tatgaaggat atggtccggg tggagtagga atcttagtcc
    18121 atgcattgac agataaccgt aatcggacag cgacaaatgt ccgagtagct tttactcgaa
    18181 acggtggatc actgggagaa acgggttcag tcagttactt gtttgaccgc aaaggatata
    18241 tcgctattga acgcgatggg ttgactgttg atgaagagac gatgtttgag gatgtattag
    18301 aggcgggggc agaagatctg caaacttctc ctgaagtatt cgagatttat actgcaccag
    18361 aagattttgc tgctgtacga gatgagttgg aaaaagagtt cactttggcg caagcagaat
    18421 tgacgatgat tccacaaacg acgattgatt taaatgagga acaaaaagag caattggaac
    18481 gtctagtaga taagctagaa gacgatgatg atgtttcaga agtctttact gctgctgaaa
    18541 tgtaagtagc acattgttag ataataaaaa gaaagcacct gtaccaatcg tacgggtgct
    18601 ttc
//
LOCUS       DACGLE010000052        18511 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0534
            SAMEA3304449-rid9560453.denovo.056, whole genome shotgun sequence.
ACCESSION   DACGLE010000052 DACGLE010000000
VERSION     DACGLE010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304449
            Sequence Read Archive: ERR985064
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18511)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18511)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702225.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702225.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 20:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,045
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 27 of 67
            Pseudo Genes (incomplete)         :: 25 of 67
            Pseudo Genes (internal stop)      :: 19 of 67
            Pseudo Genes (multiple problems)  :: 4 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18511
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304449-rid9560453.denovo.056"
                     /strain="VREN0534"
                     /db_xref="taxon:1352"
     gene            155..2344
                     /locus_tag="IXT20_001910"
     CDS             155..2344
                     /locus_tag="IXT20_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000470930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAQ4860348.1"
                     /translation="MFISTLQIRNFRNYESEIFRFDNETNTIIGENDSGKTNALTALR
                     ILLDDSYYYSSKTLKESDFFHGIQNGWQGHWIIISATFEGISEEEFDNEICASISLNS
                     ESQTILEELISNADKGVGSISLFIRPNKAIRKQLFDISTELDSHQFNEFRNSIRLSDY
                     EFYYTSKSNLNFCINENYDKLVGRLNDNIASNPEEDDEALLGSRIDMSDIFKHISVVY
                     VDALRDVLREMKNNRNPVKRIMETIESKISSDNVESLKTIIQQLNETITSVPEIRKIG
                     ENINRQLNSIIGSVYSPNLLLSSTMSDDMGSLAKFLSMKPEQNIDLDLLGLGHLNMIY
                     LALKIVEFEACRSRELLNIMLIEEPEAHIHHHIQKTLFEGLNLQKNYTQILMTTHSVH
                     LAEVSEISRMNVLKSFNGKSIVMNPANNLNSFAKEKLKKNNLNLINAVERYLDVKRNG
                     LLFSKGVILVEGDAEEILIPQIVKKLFGINLDELGIGLINVGSTSFEYIASLFDDDRI
                     QRKCAIVTDLDEQAIPSEHRLYKSTAQEKGQARKEKLSSLFNENNWVNCFYANTTFEI
                     EFLDVNKENILTYVSPIIDDAFKDKKTITAYKEEIQSEQYLERNLAMLKLANHVGKGW
                     FAIELGEKIDFLIKIPKYILNAIAFACQEIVDLSIYKKILLHTIDLYEETTELSALSA
                     SLKTSDTADLNIAIISLLEYFGKDEDVVYLINQIENYTKILYAEVSK"
     gene            2341..4134
                     /locus_tag="IXT20_001911"
     CDS             2341..4134
                     /locus_tag="IXT20_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000181735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HAQ4860349.1"
                     /translation="MTNIFEKIVNDLNLEQKDVVESEDNLYVNACPGSGKTRVLTRKI
                     AYQAIKHDGSLKRIIAITYTNRAAEEIKERLSLLSIDDDINIWVGTIHQFCLEFIIYP
                     FKMNLSRVSKGFTIIDDYTQRAYVSQINNMLNLNLKTYELNKIDLSLSHDFKINEVQY
                     TQVSEMYHTMLEKNKEIDFDMILLVAYKILTVNSNVARNIASNIRAIYVDEFQDTREL
                     QYNVIAKLLQNNPQIQSMFVGDIDQAIYGSLDGIAKSVEELEMLTSHTFQSKTLHGCY
                     RSNQRLVDFYSNFQSCPYEIESRGNNKNDRGFISYDCKVTKEDLPNIIKNIIKEKIES
                     GIHEKDICVLAPQHYPLFSLGEYLKKELPYCNFDAINISPIKVNNHSLFFKLAILYFT
                     ESGEKVRRRKLIANDILIILKNEFGIEVKDYFIDLDLLQLINSVKRMYSGDDNGVDLY
                     KFVTKHIFVNLNIDEKNYPSIFKHYLFFINEVEDRIKNNSLSSSVSSFKKMFKSRDGI
                     TLTTAHKVKGNEYDTVIAINLLNGKIPHWNEIFNVSDKGISSARKLLYVICSRAKNNL
                     FLFSEVGYFTSKGDLLYPTQLLDKIEFDYNI"
     gene            4334..4648
                     /locus_tag="IXT20_001912"
     CDS             4334..4648
                     /locus_tag="IXT20_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018705633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAQ4860350.1"
                     /translation="MELKYVIPNMEKTFGQLEYAGEGNIEQRRINGRPTILSRSYNLY
                     STIQRADDIVVILPSEAGEKHFESDEKVKLINPHITAEGYKIGNRGFTNYIMHADDMV
                     KA"
     gene            4669..5046
                     /locus_tag="IXT20_001913"
     CDS             4669..5046
                     /locus_tag="IXT20_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001234189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAQ4860351.1"
                     /translation="MRLAEGIVIEKDQTFGTLKFSALRREVRIQNEDGTVSEEIKERT
                     YDLKSKGQGRMIQVSIPASVPLKEFDYNAEVELINPVADTVATATFRGADVDWYVKAD
                     DLVLKGQTKPHQKYKKEASESEK"
     gene            5056..5829
                     /locus_tag="IXT20_001914"
     CDS             5056..5829
                     /locus_tag="IXT20_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4860352.1"
                     /translation="MTNTPLILKEIPKDEAISFIRQYHYSKILPRLCKYFLGIFSEEK
                     LLGVVELGWGTQPLQTIRKLFPDSSLQTTDYLEIGKMCFLPEMNQTNYFGSQALSALI
                     KWLKEHTDCHFLYTLADGIEGKCGYVYQASNFFYCGYFKTSVYRDKQSWEKIHPRSAR
                     LLLEENARFEQVEKKHWLSQAFCEYKGIEKINGRMFRYLYPLTKEAKKLLGHTLYRRH
                     YYPKEKNLRFEKRIAYQKYEAISQPTFDKQARIYNTQLF"
     gene            5851..7254
                     /locus_tag="IXT20_001915"
     CDS             5851..7254
                     /locus_tag="IXT20_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001130245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HAQ4860353.1"
                     /translation="MPLLHDRGERIKANDKHLVYHFVLISLLVVFLVVLLPFHWRALQ
                     SVNWQAVQLDTFFYQLHTPYFWSSILTALFVCGLISWLYYHYRIDSIKQLEHRQKLAR
                     MILENHWYEAEETQSEGFFKDLPSKKTKQRIRHFPKIYYRMKDGLIHIHVEITLGKYQ
                     DQLLHLENKLETGLYCELVEKTLKDSYVEYVLLYDTIGNRIGIEDVVAQDGRVKLMES
                     VYWEFDSLPHMLIAGGTGSGKSYFILTLIESLLHTNAKLYILDPKNADLADLGAVMNN
                     VYYRKEDMLACINQFYEDMIDRSETMKQHPNYKTGENYAYLGLSANFLIFDEYVAMMD
                     MLGRESTTVIHKLKQIVMLGRQAGFFLILACQRPDAKYFSDGIRDNFNFRVALGRMSE
                     MGFGMMFGSDTQKQFFLKPIKGRGYVDTGKSVISEFYTPLVPKRYDFLGEIGKVIQKK
                     QSEPVPHEVKGTGSDLL"
     gene            7436..8620
                     /locus_tag="IXT20_001916"
     CDS             7436..8620
                     /locus_tag="IXT20_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000398284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HAQ4860354.1"
                     /translation="MENEKIWIKELKEKRLAYGVSQNKLAVASHITRPYLSDIETGKA
                     VPTQQVKEDLLNALERFNPDNPLEMLFDYVRIRFPTNDVAQIIGEVLRLNMDYMLHED
                     FGYYSYPEHYRFGDIVVLVSHDVSKGVLLELKGKGCRQFENFLLAQHRSWYDFFQDCM
                     EHKGIFKRLDLAINDKTGILNIPDLAKKCKQEECISVFRSFKNYRSGELVHRDEKPYM
                     GNTLYIGSLKSEVYFCIYEKDYEQYVKNDVPLEDAEVKNRFEIRLKNDRATQAMKDLL
                     AHQQAEKTAFEIINRYIRFADKDDTKRRSDWKTNERWEWFIGKNRGALRLTTQPEPYS
                     FERTLNWLHHQVAPTLKIASILDVLNGTTIISTMIQEAKLTEKHEKLIEQQHLAMEDL
                     IT"
     gene            8617..8907
                     /locus_tag="IXT20_001917"
     CDS             8617..8907
                     /locus_tag="IXT20_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000055367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4860355.1"
                     /translation="MSLLTKELKKLGFQCGIEFQAYIQNTGKYTSLIIEGKRQAGDTI
                     YTYDFYKVRFYNNYTNRVTVYGEHLTPFQLLRRVKSYIYYREKYLKERRTIT"
     gene            8904..9125
                     /locus_tag="IXT20_001918"
     CDS             8904..9125
                     /locus_tag="IXT20_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4860356.1"
                     /translation="MNFGQNLYNWFLDNAQSLVLLAIVVIGLFLGFKREFSKLIGFLI
                     IALIAVGLVFNAAGVKDVLLNLFNRIIGA"
     gene            9167..9946
                     /locus_tag="IXT20_001919"
     CDS             9167..9946
                     /locus_tag="IXT20_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000678782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="abortive infection family protein"
                     /protein_id="HAQ4860357.1"
                     /translation="MKGFMNMFTNDQFKSLLIKTLERKSSSNYYEGGNEIVSKMKISE
                     VTLDETDDFTYYKGYKKGWNTLCTYLKIRVPFDDLDFFESHKDLITQTASSIYDKQGD
                     NVLVDTILVPLPENYEVINFSQLKISDVVSQAIEDAESFMSNGEYQRAFDRVHTAFHG
                     YLIEILKKYEITVPRDENLSKLYSRIQQLIEKEIQPTELADIVKTTIRSSNGMISSLN
                     EARNRHSLAHPNTNIIGKREAKLIIGISSTVTDYISGYLDK"
     gene            10013..10507
                     /locus_tag="IXT20_001920"
     CDS             10013..10507
                     /locus_tag="IXT20_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000870467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HAQ4860358.1"
                     /translation="MKVYVANLGRYNEGELVGAWFTPPIDEEEMAERIGLNEDYEEYA
                     IHDFELPFDVDEYTPISEINRLCEAIQEIEGTPIYNELKEIQGMWFSSLEELLENKED
                     IHCYSDCDSMEDVARYYVEETGQLGEVPSNLQNYIDYQSLGRDMEIEGNYLVTSHGVF
                     EYCQ"
     gene            10563..11201
                     /locus_tag="IXT20_001921"
     CDS             10563..11201
                     /locus_tag="IXT20_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4860359.1"
                     /translation="MVNVFENLKLLKLDMKDKGWVIDSFYFRYKQQNYIVLVKLFEKE
                     EKVPEYALLKLEFLKENDFSDMLAVYANSVKLYTDTKTIREYFGIEYSSNLGDVLFQF
                     SQTLARFIPTEVSEKKNEDQKEAMCFSLSQSDSEDPRKKYCFSVRRNPLKGNGELGKR
                     SPFNDNKTRLYRPSLYERLGSDTNLSFRYSMNPDDEETDEGIIAKWTKNKIE"
     gene            11273..11668
                     /locus_tag="IXT20_001922"
     CDS             11273..11668
                     /locus_tag="IXT20_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAQ4860360.1"
                     /translation="MKKIRSYTSIWSVEKVIYAINDLQLPFPITFTQMTWFIVSLFVV
                     ILFGSLPPLSLIDGALLKYLGIPIVLTGFMCQKTFDGKKPYGFLKSIITYALRPKVTY
                     AGKQIRLKKETMNESITIVRSEQLDGISD"
     gene            11652..14105
                     /locus_tag="IXT20_001923"
     CDS             11652..14105
                     /locus_tag="IXT20_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAQ4860361.1"
                     /translation="MAYPIKYMEDNLVFNHDGEVFAYYELIPYNYSFLSSDEKIQVHD
                     NFCQLIAQNRDGKIHALQLATESSIRATQEQSKKEITGRLKEIAYEKIDEQTEALISM
                     IGDNQVDYRFFIGFKLYLNEQEVSVKNVGKEIQKAFAEFFHGVNHKLMGDFVSMPMAE
                     IERFSKMEQLLESKLSRRFQIRKLEKDDFGYIIEHLYGQTGVPFDDYDYHLPKRYEDK
                     KVLVKKYDILKPTRCLIEENQRYLRIEQEDTTTYVAYFTINSIVGELDFPNSEIFYYQ
                     QQQFTFPVDTSMNVEIVANKKALSTVRNKKKELKDLDSHAWESDNETSTNVVDALESV
                     DELESNLDQTKESMYKLSYVVRVSAKNLDELKQRCDEVKDFYDDLSIKLVRPFGDMLG
                     LHNEFIPSSKRYINDYIQYVTSDFLAGLGFGATQMIGENSGIYVGYNLDTGRNIYLQP
                     SLASQGIKGSVTNALASAFIGSLGGGKSFSNNMLVYYSVLFGGQATIVDPKAERGEWK
                     ETLPDIAHEINIVNLTSENDNKGLLDPYVILKLPKDSESLAIDILTFLTGISSRDSEK
                     FPVLRKAIRSVTQSKQRGLLLVIDELRKEDTPISNSIADHIESFVDYDFAHLLFSDGS
                     VTQSISLEKQLNIIQVADLVLPDSNSSFEEYTTMELLSVAMLIVISTFALDFIHSDRS
                     IFKIVDLDEAWSFLQVAQGKALSMRLVRAGRAMNAGVYFVTQNADDLLDEKMKNNIGL
                     KFAFRSTDLVEIKKTLEFFGVDKEDENNQKRLRELENGQCLVSDIYGRVGVMQFHPIF
                     EELLHAFDTRPPVRNEVEE"
     gene            14102..16129
                     /locus_tag="IXT20_001924"
     CDS             14102..16129
                     /locus_tag="IXT20_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000192391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4860362.1"
                     /translation="MTQKQKKFLHYSWITLLCVGVILLLLGTLGNAVQATGLVDETID
                     TSNEYSKYGLNQYQLDYYVDNSWGWLPWNWSDGIGQSVMYGLYAITNFIWTISLYLSN
                     ATGYLVKEAYALDFISQTSQAIGENMQTIAGITPNGFSSSGFYVGFLLLFILIVGIYV
                     AYTGLVKRETTKVIRAVLNFVVVFILSASFIAYAPDYIAKINDFSKDVSTASLNIGTK
                     IVLPNTDSQGKDSVDMIRDSLFSVQVKQPWLLLQYGSTDVEALGEERVESLLSTSPDT
                     NNGEDRENVVIEEIEDHDNTYLTLPKTISRLGTVFFLFVFNIGISIFVFLLTGIMIFS
                     QVLFIIFAMFLPISFLLSMLPSFDGMGRHAITKLFNVIMTRAGITLIITVAFSISTML
                     YSLSASSPFFMIMFLQIVTFAGIYFKLGDLMSLFTLQSNDSQNMGRQVFRRPRQMMNR
                     QTRRFQRSMSKIFRGKAGSTKQKTMTTKQNQTNKANHTRQNERTTPKKETALSNVKKQ
                     QGGIGQRLGEKTAHVLDTKNRLVNKRKQAKQQIQATPTHVKYALHQGKENVTHNVTDF
                     KQSMADTKAQRKQQRMNQQEQKRKNIAQKRLELDKAKEKRRKENPSSPVQNNQQHVRK
                     RQQTVTPVLSQNTKRENHQRPLKKEQKQKTPPKQFNQRKLNKRTDKRGQQT"
     gene            16126..17148
                     /locus_tag="IXT20_001925"
     CDS             16126..17148
                     /locus_tag="IXT20_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000768374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase P60"
                     /protein_id="HAQ4860363.1"
                     /translation="MKLKITVICSVVFSLFFFLLLFLVIFFADDTDSGENNKDSSIPQ
                     GGVTVSPEVLAHRPLIEKYGKEYGIEDYVSYILAIMQVESGGTAEDVMQSSESLGLPP
                     NSLSTEESIKQGVKYFSELLTSAEQQGVDIDSVIQSYNYGGGFLNYVRSHGKKYTYEL
                     AEQFSKEKSGGQKADYPNPIAIPVNGGWRYNYGNQFYVQLVSQYLTDTSPTEFDDETV
                     QVIMDEALKYEGFPYVFGGASPTTSFDCSGLIQWVYDKAGISLPRVAQDQYDATQEIS
                     MEEAQAGDLIFFHSTYNAGTYVTHVAIYLEGNRFYHAGDPIGYGDLSSRYWQDHLIGA
                     RRVIHN"
     gene            17165..18094
                     /locus_tag="IXT20_001926"
     CDS             17165..18094
                     /locus_tag="IXT20_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAQ4860364.1"
                     /translation="MIIKIERKEKKEKPKKSKKPSKQRKVPMIKVGTHKKLTFVLWVL
                     LIGSVGFGIYKNFTAIDTHTVRETEIIKQQIVDTNQVESFVKSFAKDYFSWQQSQEAI
                     DKRNEKLTHYLTEELQVLNEEMIRKDIPTSSSVNDIQVWQVSQVNENTFEVLFSVEQV
                     ITEDKDKETISSSFHVVVHIDESDNMVIIKNPTMSKKPQKSDYQPKQLESDHTVDTET
                     MDEITSFLETFFQLYPTATEKELTYYVSNHVLPMINKEYVFEELVNPIFTRKDNQVIV
                     NVAVKYLDQETKATQISQFELILEKQDNWKIVK"
     gene            18339..18455
                     /locus_tag="IXT20_001927"
     CDS             18339..18455
                     /locus_tag="IXT20_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance determinant leader
                     peptide"
                     /protein_id="HAQ4860365.1"
                     /translation="MILKYPENICMLCIPMVMHKNPSDKSIYHWDFYALLGF"
BASE COUNT         6424 a         2966 c         3547 g         5574 t
ORIGIN      
        1 agccaactga ttgagaatta gaaacaatct gcagtaggga agaatcattt acacaaaatt
       61 attgacgctc ccttataatg agaactttta ctaacataat ttatgataaa cttactatat
      121 aatcatatta attaaatagt taggagatct gcttatgttt atatctactc ttcaaattcg
      181 aaattttaga aattatgaat ccgaaatctt tcgattcgat aatgagacaa atactattat
      241 aggtgagaat gattcaggga aaaccaatgc tcttactgca ttgagaatct tattagatga
      301 tagttattac tatagtagta aaacactaaa agaatcagat ttttttcatg gaattcaaaa
      361 tgggtggcaa gggcattgga ttattatctc tgcgacattt gaaggaatca gcgaagaaga
      421 atttgacaat gagatttgtg cttccatttc tttaaattct gaaagtcaaa caatcttaga
      481 agaattgatt tcaaatgcag ataaaggtgt aggtagtatt tctctattta ttagacctaa
      541 caaagctatt agaaagcaat tatttgatat ttccactgaa ttagactctc atcaatttaa
      601 cgaatttaga aattctattc gtctatccga ctatgaattt tactacacat ctaaatcaaa
      661 tctaaatttc tgtatcaatg aaaattatga taaattggta ggaagactca atgacaatat
      721 agctagtaat cctgaagaag acgatgaagc tctgttgggc tcaagaattg acatgtcaga
      781 tattttcaag catatttcgg ttgtttatgt tgacgctctt cgagatgttc ttagagaaat
      841 gaaaaacaat cgaaacccag tcaaaagaat catggaaaca attgaatcaa aaatatcatc
      901 agataatgtt gagtcattaa aaacaattat acagcaatta aatgagacta ttaccagtgt
      961 tcctgaaatt aggaaaattg gcgaaaatat taatcggcaa ctaaatagca taattggttc
     1021 agtgtattct cctaatcttc ttctttcctc aactatgtca gatgatatgg gttctttagc
     1081 aaaatttctt tcaatgaagc cagaacaaaa tatcgactta gacttactag ggttaggaca
     1141 tctaaatatg atttatttag ctttgaaaat agtcgaattt gaagcgtgtc gttcaagaga
     1201 actacttaat attatgttga ttgaagaacc agaagctcat attcatcatc atattcaaaa
     1261 aactctgttc gaaggattaa atcttcaaaa aaactatact cagattctca tgaccacaca
     1321 ttctgtacat ttagctgaag tgtctgaaat atctcggatg aatgtattaa aatcgtttaa
     1381 tggcaaatct atcgttatga atccagcaaa taatctgaac tcatttgcta aagaaaagtt
     1441 aaaaaagaac aatctaaatc taataaacgc agtagagaga tatttagatg ttaaaagaaa
     1501 tggattgctt ttttctaaag gtgttatctt agttgaagga gatgctgaag aaatacttat
     1561 tcctcaaata gtaaaaaaat tatttggtat aaacttagac gaacttggaa taggtttgat
     1621 taatgttggt agtacttcgt ttgaatatat cgctagcttg tttgacgatg atagaataca
     1681 gagaaagtgt gctattgtaa ctgatttaga tgaacaggca atcccatcag aacatcgatt
     1741 gtataaaagc actgcacaag agaaaggtca agcaagaaaa gaaaaattat cctccctttt
     1801 taatgaaaac aattgggtta attgttttta tgctaataca acatttgaaa ttgagttttt
     1861 agatgtgaat aaagaaaata ttctaactta tgtcagtcca attatagatg acgcattcaa
     1921 agataaaaaa acaataactg cttataaaga agaaatacaa agtgaacaat atttagaaag
     1981 aaatttagcg atgttaaaac tagccaatca tgttggtaag gggtggtttg caattgagtt
     2041 aggtgaaaaa attgactttt taatcaaaat tcctaagtat attcttaatg ctattgcttt
     2101 tgcatgtcag gaaatagtag atctctcaat ttataaaaaa attctactcc atacaatcga
     2161 cttgtacgag gaaactacag aattgtcagc tttatcagct agtttaaaaa caagtgatac
     2221 agctgatttg aatatagcaa taatttcttt attagagtac tttggtaaag atgaggatgt
     2281 tgtgtatcta atcaatcaga tagaaaatta cacaaaaata ctttatgctg aggtttcaaa
     2341 atgacaaata tcttcgaaaa aatcgtaaat gatttaaatt tagaacaaaa agatgttgtg
     2401 gaaagtgaag ataaccttta tgtaaatgca tgtcctggga gtgggaaaac aagggtactt
     2461 acaaggaaaa ttgcttacca agcaattaaa cacgatggat cactaaaaag aataattgct
     2521 atcacataca ctaatagagc tgctgaagaa attaaggaac gcctaagttt acttagtata
     2581 gatgatgata ttaatatttg ggtggggaca atacatcagt tttgtcttga atttattatt
     2641 tatcctttta aaatgaatct ctcaagagta tcaaaaggat ttacaattat tgatgattat
     2701 actcaaagag cttacgtcag ccaaattaat aatatgctga atttaaatct caaaacgtat
     2761 gaactgaaca aaattgattt gtcactttct catgatttta aaattaatga agttcagtat
     2821 actcaagttt ctgaaatgta tcacactatg ttggaaaaaa ataaagaaat tgattttgat
     2881 atgattttac tagtagcgta taaaatttta actgtaaaca gtaacgttgc acgtaatatt
     2941 gctagtaata taagggcgat ttatgtggat gaatttcaag atactagaga attacaatac
     3001 aatgtaatag ctaaattact tcaaaacaac cctcaaattc agtcaatgtt tgttggagat
     3061 attgaccaag caatttatgg atcattagat ggtatagcta aatcagttga agaactagaa
     3121 atgctaacta gtcacacttt tcaatcaaag acgctacatg gctgttacag gtctaatcag
     3181 agattagtag acttctactc taattttcag agttgtcctt acgagattga aagcagaggg
     3241 aataataaaa acgaccgtgg ttttataagt tatgattgca aagttactaa ggaggattta
     3301 cccaacataa taaaaaatat aataaaggaa aagattgaaa gcggcattca tgaaaaagat
     3361 atttgtgtac ttgctcctca acactatcct ctgttttcac tgggtgagta tttaaagaaa
     3421 gaattaccgt attgcaattt tgatgctata aatatatcac caattaaggt aaataatcat
     3481 agtttatttt tcaagttagc aattttgtac tttactgaat caggtgaaaa ggttcgaaga
     3541 agaaagctaa ttgcaaacga tattttaatt atattaaaaa atgaatttgg aatagaagtt
     3601 aaagattatt ttattgactt agatttatta cagctaataa acagtgtgaa aagaatgtat
     3661 agtggagacg acaacggtgt tgatttatac aaatttgtta ccaaacatat atttgttaat
     3721 cttaatattg acgagaaaaa ttacccgtct atttttaagc actatctatt ttttattaac
     3781 gaagtagaag atcggataaa aaataattca ttatctagta gtgtatctag ttttaagaag
     3841 atgtttaaat ctcgagatgg tatcacactt actacagcac ataaagttaa aggaaatgag
     3901 tatgatactg ttatcgctat taatcttcta aacgggaaaa ttcctcattg gaatgaaatt
     3961 tttaatgttt ctgataaagg aattagttca gcgagaaaac tactatacgt aatttgttca
     4021 agagcaaaaa ataatttatt tcttttctca gaggtgggtt attttacgag taaaggagac
     4081 ttgctttatc ccactcaatt attagataaa atagaatttg attataatat ttaaaatacc
     4141 taggtagtcg ttgttttaaa tatagtagga atcggacgct cagtacggag cgtctatttt
     4201 tttacccaat tttgaaagga agtgaactta tgaaaataaa aaatcaagaa tcaaaaggtc
     4261 gctctccccc ctttaaaacc atcacacaaa taagattcat tcaaacataa aaacagaaag
     4321 gataggtaaa aatatggaac ttaaatatgt cattcccaat atggaaaaaa catttggaca
     4381 attagaatat gctggtgaag ggaacattga acaacgtcga ataaatggca gaccaaccat
     4441 tctctctcgt agttacaatt tatactcaac gattcaacga gcagatgata ttgttgtcat
     4501 tcttccctcc gaagctggcg agaaacattt tgaatccgac gaaaaagtca aactcatcaa
     4561 cccacatatt accgcagaag gttataaaat tggcaatcga ggatttacca attacattat
     4621 gcacgcagac gatatggtga aagcataaga aaggaaaggt gaaaaaatat gagattagca
     4681 gaaggtattg tgattgaaaa agaccagaca tttggcacat tgaaattttc cgccttgcgt
     4741 cgtgaggttc gtatccagaa tgaagatggt acggtttccg aagaaattaa agaacgtacc
     4801 tatgacttaa aatcaaaagg acaaggacgc atgatacaag tcagtatccc cgcttctgtt
     4861 cctttgaaag aatttgacta caacgcagaa gtggaattaa tcaatcctgt ggcagatacc
     4921 gtagcgacgg ctacttttcg aggggcagac gttgattggt acgtgaaagc agacgatctt
     4981 gtattaaaag gacagacaaa acctcatcag aaatataaaa aagaagcgtc cgaaagtgaa
     5041 aaataggtgt tgcttatgac aaacacacca ttgattttga aagaaattcc gaaggatgaa
     5101 gccatttctt ttattcggca ataccattat tcaaagatac tccctagatt gtgtaaatac
     5161 tttctaggga ttttttcaga agaaaaatta ttaggcgttg tggaattagg ctggggtacg
     5221 caacccctac aaaccatacg caaactcttt cccgacagtt cccttcaaac cacagattat
     5281 ttagaaattg gaaagatgtg ttttcttcca gaaatgaatc aaaccaatta ttttggcagt
     5341 caagctcttt cagctctaat taagtggttg aaagaacata cggactgtca ttttttatat
     5401 acactggcag atggaattga gggaaaatgt ggctatgtct atcaagcgtc caacttcttt
     5461 tactgcgggt atttcaagac aagtgtctat cgtgataagc agtcatggga aaagatacac
     5521 cctcgaagtg ctcgcctttt attagaggaa aatgctcgct ttgaacaggt agagaaaaag
     5581 cattggcttt cccaggcatt ttgcgaatac aaagggattg agaaaatcaa tggacgcatg
     5641 tttcgctatc tctatccgtt gacaaaagaa gcgaaaaaac tgttaggaca cactctctat
     5701 cgacgtcatt attatccgaa agaaaaaaat ttacgctttg aaaagcgaat cgcttatcag
     5761 aaatatgaag ccatttctca accaactttt gataaacagg ctcgtatcta caatacacaa
     5821 ttattttgat aggtggaaag gaggattttt atgccattgc ttcatgacag aggagagcga
     5881 atcaaagcca acgataagca cttggtctat cattttgtac taatttcatt gctggtagtt
     5941 tttctagtcg tcctactccc ttttcattgg agagcgttac aatcggtgaa ctggcaagca
     6001 gttcagttag atacattttt ctatcaactt catacgccat acttttggag cagtattctc
     6061 actgctcttt ttgtatgcgg acttatcagc tggctttatt atcactatcg tatagattct
     6121 ataaaacagt tagagcaccg tcaaaagtta gctcgtatga ttttagaaaa tcattggtat
     6181 gaagccgaag aaactcaatc agagggattc tttaaagatt tacccagcaa gaaaaccaaa
     6241 caacgaatcc gccattttcc taaaatatat tatcgaatga aagatggact aatccatatt
     6301 catgtagaaa tcaccttagg gaaatatcaa gatcagcttt tgcacctaga aaacaaacta
     6361 gaaacaggat tgtattgtga attagtggaa aaaacgttga aagattctta tgtagagtat
     6421 gtcctacttt atgatactat tggcaatcga attggcattg aagacgtggt agctcaagat
     6481 ggacgtgtca aattgatgga atcggtctat tgggagttcg attctctccc tcacatgtta
     6541 attgctgggg gcactggtag tggaaaatcc tattttattc tcacattgat tgaatctctc
     6601 ctgcatacaa atgcaaagct ttatattctt gaccctaaaa atgctgattt ggcagactta
     6661 ggggcggtca tgaataatgt ctattaccgt aaagaagata tgttggcatg tatcaatcaa
     6721 ttttacgaag atatgattga tcgcagtgaa accatgaaac aacaccctaa ctataaaaca
     6781 ggagaaaatt atgcttatct tggtttgtct gccaattttc ttatttttga tgaatatgtg
     6841 gcaatgatgg atatgctagg tcgtgaaagt accaccgtta ttcataaact caaacaaatt
     6901 gtaatgttag gacgacaagc aggcttcttt ttaattcttg cctgtcaacg tccagacgca
     6961 aaatatttta gtgatgggat tcgggacaac tttaacttcc gtgtggcttt gggtcgtatg
     7021 tctgaaatgg gctttggtat gatgttcgga agcgatacac aaaaacaatt tttcttaaaa
     7081 cccatcaagg gaagaggtta cgtggataca ggaaaaagtg tgattagtga gttttataca
     7141 ccccttgtac caaaaaggta cgatttctta ggagaaatag gaaaagtcat tcaaaaaaag
     7201 caatctgagc ctgtaccgca cgaagtgaaa ggtacaggtt cagacttgct ttgattggtg
     7261 tggcgttagc cacgccaatc ctttagcccc ctctatctaa caggggggca caaatacaac
     7321 tgaaaacagg caattctcat caaccaatgt tgatggtaca aaggattgcg gaaaatatga
     7381 aaggtgtaaa ctcaagggtc atagtttaca tcttttttag attggaggtt tgcgaatgga
     7441 aaacgaaaaa atttggatta aggaattgaa agaaaagcga ctggcttatg gcgtttctca
     7501 aaataaactg gcggttgctt ctcatattac cagaccttac ctatcggata ttgaaacagg
     7561 caaggcagtc cctactcaac aggtgaaaga agatttactg aacgctttgg aacgcttcaa
     7621 tcccgataac ccattggaga tgttattcga ctatgtacgg attcgattcc ctaccaatga
     7681 tgtggctcaa attatcggtg aggttttacg tttgaatatg gattatatgc tacacgaaga
     7741 ctttggctat tattcttatc ccgaacacta tcgtttcggt gatattgttg tcctagtctc
     7801 tcatgatgta agtaaaggtg ttttattaga actgaaagga aaaggctgtc ggcaatttga
     7861 aaactttcta ttggctcaac atcggagctg gtatgatttc tttcaagatt gcatggaaca
     7921 taaaggtatt ttcaaacgct tggatttagc tatcaacgat aaaacaggga ttttgaatat
     7981 tccagatttg gcaaagaaat gtaaacagga agaatgcatt tccgtctttc gaagctttaa
     8041 aaattatcgc agtggtgaat tggttcaccg tgatgaaaaa ccatacatgg gaaacacact
     8101 ttatattggc tcattaaaaa gtgaagtcta tttctgtata tatgagaaag attatgagca
     8161 atacgtgaaa aatgatgttc ccttagaaga cgcagaagta aagaatcggt ttgaaattcg
     8221 gttaaaaaat gaccgagcca cccaagccat gaaagattta ttggcacatc aacaggcgga
     8281 aaaaacagcc tttgaaatta tcaatcgcta tatccgcttt gcagataaag acgacactaa
     8341 acgtcggagt gattggaaaa ctaatgaacg ttgggaatgg tttatcggta aaaatcgtgg
     8401 agcattacgc ctaaccacac agccagagcc ttattctttt gaacgaacat tgaactggtt
     8461 acatcatcaa gtcgccccta cattaaaaat cgcaagcatt ttagatgttc tcaacggtac
     8521 aaccattatc tccaccatga ttcaggaagc caaactgaca gaaaaacatg agaaattaat
     8581 tgaacaacaa catctagcca tggaggattt gattacatga gccttttaac aaaagaatta
     8641 aagaagttag ggtttcagtg tggcattgag tttcaagcgt atatacaaaa tacagggaaa
     8701 tatacgtctc tcattattga gggaaaacgg caagctggtg ataccatcta tacctatgat
     8761 ttttacaagg tgagatttta taataattat accaatcgag taaccgtcta tggagagcat
     8821 ttaacccctt ttcaattatt aaggcgtgta aaaagctata tctattatcg agaaaaatat
     8881 cttaaggaaa ggaggacaat cacatgaatt ttgggcagaa tttgtacaac tggttcttgg
     8941 ataatgcaca gtcattggta ttgttagcga ttgttgtcat tggactgttt ttaggtttta
     9001 aacgagaatt ttcaaaactc attggcttct tgattattgc tctaatcgcc gtcggacttg
     9061 tgttcaatgc agctggcgtg aaagatgtgt tattaaactt atttaaccga atcattggtg
     9121 cttaacagat atttttcatg ataaaatagg aagtatatat tttgttatga aaggatttat
     9181 gaatatgttt acaaatgatc aatttaaatc tcttttaatt aaaacattag aaagaaagag
     9241 ctcttcaaat tattatgaag gaggtaacga gattgtttct aaaatgaaaa tatcagaggt
     9301 aactttagat gagactgacg attttactta ttataaagga tataaaaagg gctggaatac
     9361 gctttgcaca tatcttaaaa taagagttcc ttttgatgat ttagactttt ttgaaagtca
     9421 taaagatttg attacccaga cagcaagctc catatatgat aaacaaggtg ataatgtatt
     9481 agtggatact attttagtac cacttccaga gaattacgaa gtaattaact ttagtcagtt
     9541 gaaaattagt gatgtggttt cacaagctat tgaagacgct gagtctttta tgagtaatgg
     9601 tgaatatcaa cgtgcctttg acagagttca cactgctttt catggatatt taattgaaat
     9661 actaaaaaaa tatgaaatta cagttcctag agacgaaaat ctatctaaat tatatagtag
     9721 gatacaacaa ttaattgaaa aagaaattca acctactgaa cttgctgata tagtgaagac
     9781 aacgataagg tcatcaaatg gaatgattag ctctcttaat gaagcaagaa ataggcattc
     9841 acttgcacac cctaatacaa atattattgg aaagagagaa gcaaaactaa taataggtat
     9901 ttcaagtaca gttacagatt atattagcgg ataccttgat aagtagcgtc attaactgat
     9961 tgctactttt tttatgacta tttttataaa aaggatgtga taagaattgg aaatgaaagt
    10021 ctatgtggca aatttaggac ggtataatga aggtgaatta gtcggagcat ggtttactcc
    10081 accaattgat gaggaagaaa tggcagaaag aatcggttta aatgaggact acgaagaata
    10141 tgcaatacat gattttgagc ttccttttga tgtggacgaa tacacgccga ttagtgaaat
    10201 caaccgtcta tgtgaagcca ttcaagaaat cgaaggtaca cctatttata atgaactcaa
    10261 agaaattcaa ggcatgtggt ttagtagctt ggaagaatta ctggaaaaca aagaggatat
    10321 tcattgttat agtgattgtg attcgatgga agatgtcgcc cgttattacg tggaagaaac
    10381 aggacagctt ggtgaagtcc cttccaattt acaaaattat attgactacc aatctttggg
    10441 acgtgatatg gaaatagagg gtaattacct agttacctct catggggtat ttgaatattg
    10501 tcagtaagtt taacaatccg tgttataatt aaattaataa atagcacgga gaggtataaa
    10561 ttatggtaaa tgtatttgaa aatcttaaac tattaaaact agacatgaaa gataaaggct
    10621 gggtgataga ttccttttac tttcgttaca aacagcaaaa ttatattgta cttgttaagc
    10681 tatttgaaaa agaggaaaaa gttccagaat atgctctgtt aaaactagaa tttttaaagg
    10741 aaaacgattt ttcagatatg ctagctgtct atgctaattc ggtaaaatta tatacagaca
    10801 cgaaaacaat aagagaatat tttggtatcg aatacagtag taacttggga gatgttttat
    10861 ttcaatttag ccaaactctt gctcgattta ttccgacaga agttagtgag aagaaaaatg
    10921 aagaccaaaa agaagccatg tgtttctcat taagtcaaag tgattcagaa gatcccagaa
    10981 aaaaatattg tttttcagtt agaagaaatc ctttaaaggg aaatggtgag ctgggaaaac
    11041 gaagtccttt taatgacaat aaaacgagat tgtatagacc cagcttatat gaacgattag
    11101 ggtcagatac caatttaagt ttcagatatt caatgaatcc agatgatgaa gaaactgatg
    11161 aaggtattat tgcaaaatgg acaaaaaata aaattgaata gttaaagcat tgaaagagca
    11221 atcacctatt ttgattgttc tttttctatt tttacggaaa ggacgtgaag acatgaaaaa
    11281 aataagaagt tacaccagta tttggtcagt agaaaaagtg atttatgcga ttaatgattt
    11341 acaactccct ttcccaatta cctttacgca aatgacatgg tttatcgttt ctttgtttgt
    11401 cgtcatactc tttggcagtt taccaccact ttctctgatt gacggagcac tactcaaata
    11461 cttagggatt cccattgttc tcactgggtt catgtgccaa aagacctttg acggaaagaa
    11521 accctatgga tttctaaagt ctatcattac ctatgctcta cgccctaaag taacctacgc
    11581 tggcaaacaa atacgattaa aaaaagaaac aatgaatgaa tcgattacga ttgtaaggag
    11641 tgaacaacta gatggcatat ccgattaaat atatggaaga taatctcgta tttaatcatg
    11701 atggcgaagt attcgcttat tatgaattaa ttccctataa ttacagtttt ttaagttccg
    11761 atgaaaaaat tcaagttcat gataatttct gtcaattgat tgcccaaaat cgtgatggca
    11821 agattcatgc tctgcaacta gcaaccgaat caagtattcg agccacacag gaacaatcga
    11881 aaaaagaaat cactggacga ttaaaagaga tagcttacga aaaaatagat gaacagaccg
    11941 aagcgttaat ttctatgatt ggtgacaatc aagtagacta tcgctttttt attggtttta
    12001 aactctacct caatgaacag gaagtatccg taaaaaatgt tgggaaagaa attcaaaaag
    12061 catttgcgga attctttcat ggtgtgaatc acaaattaat gggcgatttt gtttctatgc
    12121 ctatggcgga aatcgaacgc ttttctaaga tggaacagtt gttagaaagt aaattatcca
    12181 gacgctttca gattcgtaaa ttggagaaag atgattttgg ttatattatt gaacatcttt
    12241 acgggcaaac aggtgtccct tttgatgact acgactacca tttaccgaaa cgatacgaag
    12301 ataaaaaagt gttggtaaag aaatacgata ttctcaaacc cactcgctgt ttaatcgaag
    12361 aaaatcaacg ctatttacgg attgaacagg aagataccac tacttatgtg gcgtatttta
    12421 caattaatag tattgttggt gaattggatt tccctaatag tgaaattttt tattaccagc
    12481 aacagcagtt tactttcccc gttgatacca gtatgaatgt tgaaattgtc gccaataaaa
    12541 aagccttatc taccgttcgc aataaaaaga aagaactgaa agatttagat agccacgcat
    12601 gggaaagcga caacgaaacc agtactaatg tggtggacgc tttagaatct gtcgatgaat
    12661 tagaaagtaa tttagaccaa accaaagaat ctatgtacaa actaagctat gtggtacggg
    12721 tatcagcaaa gaatttggac gagctgaaac aacgttgtga tgaagtaaaa gatttttatg
    12781 atgatctgtc tatcaaatta gtacgacctt ttggcgatat gctaggcttg cataatgaat
    12841 ttattccctc aagcaagcga tatatcaatg actatattca atatgtcacg tcggattttt
    12901 tggctggtct tggtttcggt gctacacaaa tgattggtga aaattctggt atttacgtcg
    12961 gttacaatct cgatacaggc agaaatattt atctccaacc tagccttgca agtcaaggaa
    13021 ttaaaggctc tgtaacaaat gcattagcgt ccgcttttat tggttcatta ggtggcggaa
    13081 aatcattctc taacaatatg ctggtctatt attcagtact ctttggcgga caggcaacca
    13141 ttgttgaccc aaaagctgaa cgtggtgaat ggaaagaaac cttacctgat attgcccatg
    13201 aaatcaatat tgttaatctc acgagtgaga acgacaataa agggttactt gacccttatg
    13261 tgattttaaa actcccgaaa gattcagaaa gccttgccat tgatattctc acgttcttaa
    13321 caggtatttc cagccgagat agcgagaagt ttcctgtcct gcgtaaagct attcggagtg
    13381 taactcaaag caaacaacga gggttgttat tggtgattga tgaattgcga aaagaagata
    13441 cgcccatcag caatagtatt gctgaccata tcgaaagctt tgtcgattat gattttgccc
    13501 atctgttatt cagtgatggc agtgtcaccc aatccattag cttggaaaaa caactcaata
    13561 ttattcaagt ggcagatttg gtcttacccg attccaatag ctcttttgaa gaatacacta
    13621 ccatggaatt attaagtgtg gcaatgctga ttgtcattag tacctttgct ttagacttta
    13681 tccactctga ccgttccatt tttaaaattg ttgaccttga tgaggcttgg agttttttac
    13741 aagtggcaca aggaaaagct ctatctatgc gattggttcg agcaggacgt gccatgaatg
    13801 caggggtcta tttcgttact caaaacgcgg acgatttact agatgaaaag atgaagaata
    13861 atattggctt gaaatttgcc tttcgttcca ctgacctagt ggaaatcaaa aagaccttag
    13921 aattttttgg tgtggacaag gaagatgaaa acaatcagaa acgcttacgt gaattagaaa
    13981 atggacaatg tttagtttca gacatctatg gtcgtgtagg ggttatgcag tttcacccga
    14041 tttttgaaga gttgcttcat gcctttgata ccagaccgcc tgtgcgaaat gaggtggaag
    14101 aatgacacaa aaacaaaaga aatttcttca ctatagttgg ataacgctcc tttgtgtggg
    14161 cgttatttta cttttattag gcacgctggg caatgcagta caagcgacag gcttagttga
    14221 tgaaaccatt gatacaagca atgaatactc caagtatggt ttgaatcaat atcagcttga
    14281 ttattatgtg gacaatagct ggggctggct tccatggaat tggagtgatg gcataggaca
    14341 atcggtgatg tatggattgt atgccattac taattttatt tggacaattt ctctttatct
    14401 atcgaacgcc acaggttatt tggtgaaaga agcctatgcc ttagatttca ttagtcaaac
    14461 atcccaagca attggcgaaa acatgcagac gattgcaggc attacaccga atggcttttc
    14521 cagttcaggg ttttatgtcg gtttcctcct tctttttatt ctcattgttg gaatctatgt
    14581 tgcctataca ggattggtta agcgagaaac gacgaaagtg atacgagctg ttttaaactt
    14641 tgtcgtggtg tttattctat cggcttcctt tattgcgtat gcacccgatt atattgcgaa
    14701 aatcaatgat ttttccaaag atgtcagtac cgccagtctg aatattggga caaaaattgt
    14761 tcttcctaac acggatagtc aagggaaaga cagtgtggac atgatacgtg acagcttatt
    14821 ctctgttcaa gtaaagcaac catggctatt gcttcaatat ggctccactg atgtagaagc
    14881 gttaggggaa gaacgtgtgg aaagtttatt gtctaccagt ccagatacaa acaatggtga
    14941 agatagagaa aatgtagtga ttgaagaaat tgaagatcat gacaatacct atttaaccct
    15001 accgaagacg atttccagat taggaacagt cttctttttg tttgtattca atattggtat
    15061 ctcgattttt gttttccttc taacaggaat catgattttt tcgcaagtgc tatttattat
    15121 tttcgctatg tttttgccaa tcagtttcct attaagtatg ttgccgagtt ttgatggcat
    15181 gggcaggcat gccattacca agttatttaa tgtgattatg acaagggctg gtattacctt
    15241 gattattacc gttgccttta gtatttccac tatgctttat tcgctgtcag caagttctcc
    15301 ctttttcatg attatgtttt tacaaattgt gacctttgca gggatttatt ttaaactggg
    15361 cgatttaatg agcctattca ctttacaaag caacgactct caaaacatgg gcagacaagt
    15421 gtttcgcaga cctcgtcaaa tgatgaatcg acaaaccaga agatttcaac gcagtatgag
    15481 taagattttt cgtggaaaag cagggtcaac gaagcaaaag acaatgacaa caaagcagaa
    15541 tcaaaccaat aaagctaacc atactcgtca aaatgaacgg actacaccaa agaaagaaac
    15601 agcactttcc aatgtgaaaa agcaacaagg cggtatcggg caacgattag gtgaaaagac
    15661 agcccatgta ttggacacga aaaatcgtct agtcaataaa agaaagcagg caaaacaaca
    15721 gatacaggcg acaccaaccc atgtcaaata tgcccttcat caaggaaaag agaatgtcac
    15781 tcataatgta acagacttta aacaatctat ggcagatacc aaagctcaac gcaaacaaca
    15841 gcgtatgaac cagcaggagc aaaaacgaaa gaacattgct caaaagcgtt tggagctgga
    15901 taaagcaaaa gaaaaaagac gaaaagaaaa cccatcatct cctgttcaga ataaccagca
    15961 gcatgttcgc aagcgtcagc aaaccgtcac gcctgttttg tcacaaaaca ctaaacgtga
    16021 aaatcatcag cgaccattga aaaaagagca aaaacaaaag acaccaccga agcaattcaa
    16081 tcagaggaag ctaaataaaa gaacggataa gcgaggacaa caaacatgaa gctaaaaata
    16141 acagtgattt gtagtgtggt cttctctctc ttcttttttc tcttattatt cctagtcatt
    16201 ttctttgcag atgatacgga cagtggcgaa aacaacaagg attcttctat tccacaaggc
    16261 ggtgtgaccg tttcaccaga agtgttggca caccgtccgt tgattgaaaa atatggtaag
    16321 gaatatggga ttgaagacta tgtttcttat atcttagcca ttatgcaagt ggaatcgggc
    16381 ggtacagcag aagatgttat gcaaagttcc gaatctttgg gtttaccgcc caacagtttg
    16441 agtaccgaag agtccatcaa acaaggggtt aaatatttca gtgaattatt aaccagtgca
    16501 gaacaacaag gagtggatat agattctgtt atccaatctt ataattatgg cggtggcttt
    16561 ttaaattatg tgaggagtca tgggaaaaaa tatacctatg aattagccga acagttttct
    16621 aaagagaagt cgggtggtca aaaagcagac tatcccaatc ctatagccat acctgtgaat
    16681 ggtggttggc gatataacta tggaaatcag ttttatgttc agttggtttc acaataccta
    16741 accgacactt ccccaacaga atttgatgat gaaaccgtgc aagtcattat ggacgaagcc
    16801 ttgaaatacg aagggtttcc ctatgtcttt ggaggggcgt cgcctaccac ctcttttgat
    16861 tgtagtggct taatacaatg ggtgtatgac aaagctggca tttctctccc tcgtgtcgcc
    16921 caagatcagt atgacgctac gcaagaaatc tctatggaag aagcccaagc aggcgacctt
    16981 atcttctttc attcaaccta taatgcaggc acatatgtga ctcatgtggc gatatattta
    17041 gagggaaatc gcttttatca tgcaggcgac ccaatcggtt atggcgactt aagcagtcgt
    17101 tattggcaag accatctgat tggggctaga cgtgttattc ataactaaga aaggaatgaa
    17161 tcaaatgatt ataaaaatcg agcgaaaaga aaagaaggag aaaccaaaga aatctaaaaa
    17221 accgagcaag caaagaaaag ttccgatgat taaagttggc acgcataaga aattaacctt
    17281 tgttttatgg gttttattga ttggtagtgt cggatttgga atctataaaa attttactgc
    17341 cattgacact catactgtcc gtgaaacaga aatcattaaa cagcagattg tcgataccaa
    17401 ccaagtagaa agttttgtga aatcctttgc gaaagactac ttttcgtggc aacaatccca
    17461 agaagccatt gataaacgaa atgaaaaatt gacccattat ttgacggaag aattgcaggt
    17521 attaaatgaa gaaatgattc gtaaggatat tcctactagt tctagtgtga acgatataca
    17581 ggtgtggcag gtttctcaag tgaatgagaa cacctttgag gtactgtttt ctgttgaaca
    17641 agttattacc gaagacaaag acaaggaaac tatatcgtct agctttcatg tggttgtcca
    17701 tatagatgaa tcagataata tggttattat caaaaatccg acaatgagta agaaacctca
    17761 aaaatcggac taccaaccca aacagctaga aagtgaccat acagtagata cagaaacgat
    17821 ggacgaaatc actagctttt tagaaacgtt cttccagctc tatcctaccg ccacagaaaa
    17881 agaattgact tactatgtaa gtaatcatgt gttacctatg attaacaagg aatatgtttt
    17941 cgaggaattg gtgaatccta tctttaccag aaaagataac caagtaatcg tgaatgtcgc
    18001 tgtgaaatat ttagatcaag aaacaaaggc aacccaaatc tcgcaatttg agttgatatt
    18061 agaaaaacaa gataattgga agattgtaaa ataacaaata ttggtacatg attacagata
    18121 ctttgtaatc atgtactctt tttgataaaa aattggagat tcctttacaa atatgctctt
    18181 acgtgctatt atttaagtga ctatttaaaa ggagttaata aatatgcggc aaggtattct
    18241 taaataaact gtcaatttga tagcgggaac aaataattag atgtcctttt ttaggagggc
    18301 ttagtttttt gtacccagtt taagaatacc tttatcatgt gattctaaag tatccagaga
    18361 atatctgtat gctttgtata cctatggtta tgcataaaaa tcccagtgat aaaagtattt
    18421 atcactggga tttttatgcc cttttgggtt tttgaatgga ggaaaatcac atgaaaatta
    18481 ttaatattgg agttttagct catgttgatg c
//
LOCUS       DACGLE010000053        18503 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0534
            SAMEA3304449-rid9560453.denovo.057, whole genome shotgun sequence.
ACCESSION   DACGLE010000053 DACGLE010000000
VERSION     DACGLE010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304449
            Sequence Read Archive: ERR985064
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18503)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18503)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702225.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702225.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 20:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,045
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 27 of 67
            Pseudo Genes (incomplete)         :: 25 of 67
            Pseudo Genes (internal stop)      :: 19 of 67
            Pseudo Genes (multiple problems)  :: 4 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18503
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304449-rid9560453.denovo.057"
                     /strain="VREN0534"
                     /db_xref="taxon:1352"
     gene            99..746
                     /locus_tag="IXT20_001928"
     CDS             99..746
                     /locus_tag="IXT20_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HAQ4860366.1"
                     /translation="MREKTDTLPKATAKRLPLYLRYLKMLDDSGISRIKSNEFSEITQ
                     IPSATIRRDFSQLGELGRSGYGYDVPFLIDVFNNILNTKEEKRIALVGYGNLGKALKH
                     NNFRRNENLNIVCVFDNDPALINRVIDGEMIYPIDRFAEIAKAKNVTVAISTVPSKYS
                     QSAIDEIVKGNVTAILNFAPDRVTVPAYVNVQYIDLTTELQTLIYFDENYSEVFS"
     gene            1139..3784
                     /locus_tag="IXT20_001929"
     CDS             1139..3784
                     /locus_tag="IXT20_001929"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HAQ4860367.1"
                     /translation="MTEENNLSTKYNPQEVEAGRYQHWLEQDLFKPSGDKKAKPYSIV
                     IPPPNVTGKLHLGHAWDTTLQDMLIRQKRMQGYDTLWLPGMDHAGIATQAKVEAKLRE
                     QGISRYDLGREKFIDQVWEWKEEYASHIREQWAKMGLSLDYSRERFTLDDGLSEAVRK
                     VFVTLYEKGLIYRGEYIINWDPQARTALSDIEVIHKDIEGAFYHMSYELADGSGVVEI
                     ATTRPETMLGDTAIAVHPEDERYQALIGKKVILPLVNKQIPIIADEYVDMEFGTGVVK
                     ITPAHDPNDFEVGNRHDLPRVNVMDDDGTMNELAGKYAGMERFAARKAIVQDLKEIGR
                     LIKIDTMIHSVGHSERTGVVVEPRLSTQWFVKMAPLAKKAIDNQETDQAVEFFPPRFN
                     QTFLRWMENVHDWVISRQLWWGHQIPAWYHKETGEMYVGMEAPADSENWVQDPDVLDT
                     WFSSALWPFSTMGWPDTESEDYKRYFPTSTLVTGYDIIFFWVSRMIFQSLEFTGERPF
                     ENVLIHGLIRDEQGRKMSKSLGNGIDPMEVIEKYGADALRWFLSNGSAPGQDVRFSYE
                     KMDASWNFINKIWNASRFVIMNIEGMTVEEIDLSGEKSVADRWILTRLNETIERVTEL
                     FDRFEFGEAGRQLYNFIWDDFCDWYIEMSKEVLYGDDAVSKQTTRSILVHTLDQILRL
                     LHPIMPFVTEEIWEHIPHQGNSLVVAEYPVVRPEFDDETASKGMEVLKELIRAVRNIR
                     SEVNTPLSKPITLLIKTNDAKIDQFLVENTSYIERFCNPEELTISSEITAPDLAMSAV
                     LTGAEIFLPLAGLINIEEEIKRLEKELEKWTSEVKRVQGKLSNERFVSNAPEEVVEAE
                     RAKEKDYLEKQTAVKERIESLRTIQ"
     gene            4094..5407
                     /locus_tag="IXT20_001930"
     CDS             4094..5407
                     /locus_tag="IXT20_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HAQ4860368.1"
                     /translation="MTIEEAIEWIHSRLPFGSRPGLDRVEALLEVVGHPERKVKTIHI
                     AGTNGKGSTVAYLRSLLEEAGLTVGTFTSPYIETFNERIAINGKSIPDNDLIQLVKKY
                     QPLIKQLDQFEAVAGITEFETLTAMAFDYFLMKKVDVAIIEVGLGGLLDSTNVAVPML
                     TGITTIGLDHTEILGDTIEEIAAQKAGIIKQGVPVVTGNIAPEAFTVIQQTAKAKNAP
                     LYVYGENYKINYLHPDEMWGEVFEFFNEIGKIPQLTTPLLGQHQAENAGVAIELYFVY
                     CQLEKLPFREKDVLKGLKKSRWPGRMERLSNEPLVVLDGAHNTHAMNRLVENLKKEFK
                     GYQINILFSALRTKNVREMLERLQQVPNVHIYLTTFEHPKAIELSEVSDLASERVSIV
                     SLWQFGLADILEKMTNEDVLVVTGSLYFISEVRHLLIQLGGDHEV"
     gene            5397..6050
                     /locus_tag="IXT20_001931"
     CDS             5397..6050
                     /locus_tag="IXT20_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAQ4860369.1"
                     /translation="MKYNAVIFDMDGLLFDTELVYYEATQVVADKMGFPYDKDLYLKF
                     LGVSDEEVWANYHMIFSEFGKTSVQRFIDDSYQETLERFSQGAVQMKPGVLELLSFLE
                     EHQIPKVVASSNQRAVIELLLKKNNLFERFETIVSAENVQRAKPDPEIFLLANQKLGT
                     AKKETLILEDSQNGILAASAAEIPVVMVPDLLPPSQELIKKTAGVVSSLHEIPALLK"
     gene            6200..6796
                     /locus_tag="IXT20_001932"
     CDS             6200..6796
                     /locus_tag="IXT20_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="JAB domain-containing protein"
                     /protein_id="HAQ4860370.1"
                     /translation="MAILLRTGQHPYSVMSIAGNLLKTFGGLASLRQATLHELEEIRG
                     IGRVKAIEIKALIELGRRIQTDHERAAPPVRTSYELAQQLILEMKDHKQEHLVCIYLD
                     TKNQVILKKTVFIGSLNQSVAHPREIFHYAVRYCAARIVLAHNHPSGNVIPSQQDMNF
                     TKRIQKCGEMMGITVLDHLIIGRKRYFSLREEGMMEEK"
     gene            6946..7146
                     /locus_tag="IXT20_001933"
     CDS             6946..7146
                     /locus_tag="IXT20_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAQ4860371.1"
                     /translation="MENGTVKWFNAEKGFGFISREDGSDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEESDRGPQAANVVKA"
     gene            complement(7400..9079)
                     /locus_tag="IXT20_001934"
     CDS             complement(7400..9079)
                     /locus_tag="IXT20_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HAQ4860372.1"
                     /translation="MKLSIKNNETGVFAIGGLGEIGKNTYGVQFQDEIILIDAGIKFP
                     EDDLLGIDYVIPDYSYIIQNLHKVKALVITHGHEDHIGGIPYLLKQANIPIYAGPLAL
                     ALITNKLDEHGLLRDAELHEINEDTIIRFRKTSVSFFRTTHSIPDALGIVVKTPSGNI
                     VATGDFKFDFTPVGEPANLHKMARIGEEGVLCLLSDSTNAEIPTFTKSEKTIGHSILK
                     IFSKIEGRIIFASFASNIFRLQQAADAAVATGRKIAVFGRSMENAIVNGQRLGYIKVP
                     DGTFVDAHEINRLPANETMILCTGSQGEAMAALSRIANGTHRQIQIQPDDTVIFSSSP
                     IPGNTTSVNHLINLLSEAGAEVIHGKINNIHTSGHGGQEEQKLMLRLMKPKYFIPVHG
                     EFRMLKVHASLAQDVGVPAENCFIMENGDVLALTSDSARPAGHFVAGDVYIDGSGIGD
                     IGNVVLRDRHLLSEEGLVLAVATVDIKKKEILAGPDILSRGFVYMRESGEMINEAQRI
                     LFHALRECLNGPDCSEYKLKEAMTGALQPFLFERTERHPMILPMVMTPDEE"
     gene            complement(9081..9293)
                     /locus_tag="IXT20_001935"
     CDS             complement(9081..9293)
                     /locus_tag="IXT20_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-dependent RNA polymerase auxiliary subunit
                     epsilon family protein"
                     /protein_id="HAQ4860373.1"
                     /translation="MIYKVYYQETKVRNPKREETKSLYIEAKSDVDARQRVEENTPYN
                     IEFVQLLEGSHLEYEKENADFSLVEF"
     gene            9686..11416
                     /gene="efrA"
                     /locus_tag="IXT20_001936"
     CDS             9686..11416
                     /gene="efrA"
                     /locus_tag="IXT20_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrA"
                     /protein_id="HAQ4860374.1"
                     /translation="MKLMWHYTMRYKKFLFFNFICVFGFILIELGLPTILARMIDVGI
                     RNDDFDYVKQQGIFMIVITVIGIILNILLGYFGSRITTNIVADIRDDLFEKIQSYSHQ
                     EYENLGVSSLITRTTNDAYQIMLFLQNILRIGFMAPMMFIVSLYMVMRTSVTLSLYVV
                     GALPLLLLAVVAIAKVSEPLSKKQQKNLDKINSILRENLSGLRVIRAFVNEKFEEKRF
                     AVVNENYASSSKSLFRLMAVAQPGFYFLFNIVMVLIIWSGAVQIDAGELRVGDLIAFI
                     EYIFHALFSFMLFASVFMMYPRAAVSAGRIQEALDAKPSIVEDPNGISDTKTKGYIEF
                     QNVTFAYPGHSQEPVIRNVSFTASPGETVAFIGSTGSGKSTLIQLIPRFYDVSEGKIL
                     IDGVDVRDYRLSKLRDKIGFIPQKALLFTGTIADNLRYGKEDATQEEMERAAEIAQAS
                     EFISRKPDGFNEHLSEGGSNFSGGQKQRLAIARAIIRRPEIYIFDDSFSALDYQTDAK
                     LRARLKKETTESTVLIVAQRVGTIMHADKIIVLNEGEVVGMGTHRELLEKCPIYYDIA
                     ASQLSKEELA"
     gene            11413..13173
                     /gene="efrB"
                     /locus_tag="IXT20_001937"
     CDS             11413..13173
                     /gene="efrB"
                     /locus_tag="IXT20_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_029485765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrB"
                     /protein_id="HAQ4860375.1"
                     /translation="MKKTFSSLKRLARYIRPYRETFLLVILFTVLTVAFNVALPYVTG
                     LPTTEISRNIANGESINFSYIFKCLIWITVVGIGYCISQLLSGVLMTNVVQSAMHDLR
                     RDIEEKINRLPVSYFDKNQQGNILSRVTNDVDAVSGALQQAFIGIVNAILGITLAAAM
                     MFYIQPMMALISMIMIPTSIWISKRVINASQKYFQSMQNSLGELNGYVQENMTGFSVL
                     KVYGREKETLEGFKKVNHSLKYFGFRAAFISGLMMPLVQLTAYATYIGMAVLGSFYVI
                     TGAIVVGQLQAFIQYIWQISQPMGNVTQLSSVLQSASAATKRVFEILDEPEEKENKVD
                     ASLPETILGDVTFDHVDFAYDPKKPLIQDLNFEVKAGQTVAVVGPTGAGKTTLINLLM
                     RFYDVDKGAIRIDGIDTKSMSRSDVRSLFGMVLQDAWLYQGTIADNIRFGKLDATDYE
                     VVDAAKTANVDHFIRTMPDGYDMAINSEGDNVSLGQKQLLTIARAVVSDPKILILDEA
                     TSSVDTRLEALIQKAMDRVMEGRTSFVIAHRLSTIRDADLILVMDQGQIIEKGTHESL
                     LAQGGFYEKLYNSQFAEEAE"
     gene            13268..13465
                     /locus_tag="IXT20_001938"
     CDS             13268..13465
                     /locus_tag="IXT20_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4860376.1"
                     /translation="MEQFLNEKITEAKREVDRLTHVCQTKPFDSADFFENEFQIERLA
                     AQIEVYELILKKVIEENIDVQ"
     gene            13618..14103
                     /locus_tag="IXT20_001939"
     CDS             13618..14103
                     /locus_tag="IXT20_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HAQ4860377.1"
                     /translation="MERFIASNATVIGDVTLSEDVTIWYQAVLRGDSNWIKIGQRTNI
                     QDGTIIHVDHDAPVDIAENVTVGHQCMLHGCTIEKGALIGMGTTILNHAVIGENSLIG
                     AGSLVTEGKVIPPNVLAFGRPAKVIRPLTKEEIQKNKENIQHYVEIGRMHAQGKFKEQ
                     R"
     gene            complement(14207..14800)
                     /gene="clpP"
                     /locus_tag="IXT20_001940"
     CDS             complement(14207..14800)
                     /gene="clpP"
                     /locus_tag="IXT20_001940"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HAQ4860378.1"
                     /translation="MNLIPTVIEQSSRGERAYDIYSRLLKDRIIMLSGQVTDDLANSI
                     IAQLLFLDAQDSEKDIYLYINSPGGSVTAGMAIYDTMNFVKADVQTIVMGMAASMGSF
                     LLTAGTKGKRFALPNAEIMIHQPLGGAQGQATEIEIAARHILQTRERLNKILAERTGQ
                     PLEVIEKDTDRDNYMTAEQAKAYGLIDEVMENSSSLN"
     gene            14980..15507
                     /locus_tag="IXT20_001941"
     CDS             14980..15507
                     /locus_tag="IXT20_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HAQ4860379.1"
                     /translation="MIVGITGLIGVLTGAVVVYIGMILQMRHLENKEQRRRELELKVK
                     EIETLNQLNKKINEILQKRTTLMEEYVSFDAFDDCYITIDDFAYLQSFSAQNSFYLPN
                     YILDELFKNISHRKVILSPDETMKIGGYAYKGGRVILENFTDSLTEMINEKKAQLKTM
                     TRQPIGFFSKDDVWS"
     gene            15598..16335
                     /locus_tag="IXT20_001942"
     CDS             15598..16335
                     /locus_tag="IXT20_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HAQ4860380.1"
                     /translation="MLQIYAHRGSSGTHPENTLPAFAEAVRVGADGIELDVHLSKDGY
                     LIVMHDEEVDRTTNGKGLIREKTLEELKKLNAGGKFSKEFPAAKVPELREVLGLLLQK
                     NYRGMLMIEIKTDQYEYPGIEEKLAETLKSRDWPFQHAYCSFHLDSLEKIALLEPEIQ
                     LDLLMSTSDKKIQLAAETPYIEGVHPKNTWLFEHPVQAADFPKAIRAWTINDEEEMAK
                     CQELKIDGIITDFPEKALRWKQSTKSR"
     gene            16339..17256
                     /gene="miaA"
                     /locus_tag="IXT20_001943"
     CDS             16339..17256
                     /gene="miaA"
                     /locus_tag="IXT20_001943"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HAQ4860381.1"
                     /translation="MEKVLVIVGPTAVGKTALSVELAQLFGGEIISGDSLQIYKNLDI
                     GTAKIKEEEMNNVPHHLIDVIEPTQRYSAVDFQHSGRQLITEIANRGHLPIIAGGTGL
                     YIQSLLYDYQLGATEEANPEIRKKYETLAETIGSQALWNQLKEKDPAAAEKIHWNNQR
                     KVIRALEVLETTGHSITAPKEEPKQLYDYLMIGLDTKRSILYERINQRVDLMLAEGLV
                     AEARKVYELGEVQASQGIGYKEFFPYFEGKETLEEATEQVKLHSRRYAKRQLTWFRNR
                     LDAQWFDLISEPNQKSQIEQTIKKWLEAE"
     gene            17258..18487
                     /gene="hflX"
                     /locus_tag="IXT20_001944"
     CDS             17258..18487
                     /gene="hflX"
                     /locus_tag="IXT20_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HAQ4860382.1"
                     /translation="MEEKVIIVGVETEANQRYFSESMEELVQLTNTASGEVVFTITQK
                     RPQVDRQTIIGKGKLQELVQQADAHEADLIIFNHELTPRQSQLITDAVGLPVIDRVQL
                     ILDIFAMRARSKEGKLQVELAQLEYLLPRLVGQGKTLSRLGGGIGTRGPGETKLETDR
                     RHIRNKIFAVKKELKEVEAHRERNRQKRKNSEIFQIGLIGYTNAGKSTILNLLTQADT
                     YSKDQLFATLDPLTKRWRFAEGFEITVTDTVGFIQDLPTQLIDAFHSTLEESQNMDLL
                     LHVVDASSPDRILQEQTVLKLMDELNMKEMPILTVYNKADQIDPAMFTPTLFPNVLIS
                     TQTKEGKMALIEAIKRQLMELMTPYTLSVPSNEGQKLSELRRQTMVLDEAYMEETNEY
                     EVKGFAKTNSKWLRKNQ"
BASE COUNT         5984 a         3199 c         4022 g         5298 t
ORIGIN      
        1 atttgtctca cacccttttc cttcctactt gtaaccttgc tcccgtgtta gaaacatgtt
       61 acactaaata tgtgaaatta ttaacaagga gcgtcgttat gcgagaaaaa acagatacac
      121 tgccaaaagc aactgcgaaa agattacctt tatatttacg ctacttgaag atgctggatg
      181 actcaggtat aagtagaatc aaatcaaatg agttcagcga aatcacccaa attccttctg
      241 caacgatccg cagagacttc tcgcaattag gcgagctagg cagaagcgga tatggctatg
      301 atgtcccttt tcttatcgat gtattcaata atatccttaa tactaaagaa gaaaaacgaa
      361 tcgcattagt aggatatgga aatcttggca aagcattgaa acacaacaat tttagaagaa
      421 atgaaaatct aaatatcgtg tgcgtctttg ataatgatcc agcactgatc aatcgagtaa
      481 ttgatggaga aatgatctat ccgattgatc gtttcgcgga gatagccaaa gcaaaaaatg
      541 tgacagtagc aatctctacg gtaccaagta aatattcaca atctgctatt gatgaaattg
      601 tcaaaggaaa tgtaaccgct atacttaatt ttgcgcctga cagagtaact gtcccagctt
      661 atgtcaatgt tcaatacatt gatttgacaa ccgagttgca gacgcttatt tactttgatg
      721 agaattactc agaagttttt tcttaggaaa atttgagtag gcttattgat cttgcgagaa
      781 ctcgtcgtaa ttctttcaaa aattttttag tactaaatga agtggttttt tcacgaccac
      841 ttgttgacta aaaaaaaaaa ccattgtaga atgaagcata acgctgaaca agaggagtaa
      901 ctggcacatt cattcttaga gagagaacta tctgctgaaa ggttctcaat gacaaccagt
      961 gaaggtagct tgggagtttc tgtcttgaat ggagtagaga tagacgagtc gtgatcgtta
     1021 actcacttga gaggcaataa tgattgatta ttgcaattta ggtggtaccg cgtaaatcat
     1081 acgtcctaga atacgaaagt attttaggac gttttttagt ttaaaggagg aaaacagcat
     1141 gacggaagaa aataacctat caacaaaata taatccgcaa gaagttgaag ctggacgtta
     1201 tcagcattgg ttagaacaag atttgtttaa accgagcgga gataaaaaag caaaacctta
     1261 ttcaatcgta atcccgcccc caaatgttac tggaaaactt catttagggc atgcttggga
     1321 tacgaccttg caagatatgc tgattcgtca aaagagaatg caaggatatg atactttatg
     1381 gctgcctggt atggaccatg caggaatcgc gacacaagca aaagtagaag cgaagcttag
     1441 agagcaaggg atttctcgtt atgatctagg tcgtgaaaag ttcatcgatc aagtgtggga
     1501 atggaaagaa gagtatgcga gccacatccg cgaacagtgg gctaaaatgg gcttatcttt
     1561 agattacagt agggaacgtt ttacattaga tgatggatta tctgaagcag tacgtaaagt
     1621 ttttgttacc ttatacgaaa aaggtttgat ttatcgtggt gaatatatca tcaactggga
     1681 tcctcaagca cgaacagctc tatctgatat cgaagttatt cataaagata tcgaaggcgc
     1741 cttttatcat atgagctatg aactagcaga tggatcaggc gtagtagaaa ttgcgacaac
     1801 ccgtccagaa acgatgttgg gagatacggc gattgctgtt catccagaag acgagcgtta
     1861 tcaagcgctc atcggcaaaa aagtgattct acctttagtc aataaacaaa tcccaatcat
     1921 tgcagatgaa tatgtagata tggaattcgg gacaggtgta gtaaaaatca ctccagcaca
     1981 tgatccaaac gactttgagg taggtaatcg tcatgatctt ccacgcgtaa atgttatgga
     2041 tgatgatggt acgatgaatg aactagcagg aaaatatgct ggaatggaac gttttgctgc
     2101 tcgtaaagca atcgtgcaag atctgaaaga aatcggtcga ttgattaaaa tcgatacgat
     2161 gatccacagt gtgggtcatt ccgagcggac aggtgtcgtt gttgaacctc ggctgtctac
     2221 tcaatggttt gtaaaaatgg cgccgttagc gaaaaaagcc attgataacc aagaaacaga
     2281 tcaagcagtg gaattcttcc ctccaagatt caatcaaacg tttttacgtt ggatggaaaa
     2341 tgtccatgat tgggtaatct cacgacaatt atggtgggga catcaaattc ctgcatggta
     2401 tcacaaagaa acaggtgaaa tgtatgtagg gatggaagca ccagcggata gtgaaaactg
     2461 ggtacaagat cctgatgttt tagacacttg gtttagttca gctttgtggc ctttctcaac
     2521 aatgggctgg ccagatacgg aaagcgaaga ttataagcga tacttcccta ctagcacttt
     2581 agtaaccggt tatgacatca ttttcttctg ggtaagcaga atgattttcc aaagcttgga
     2641 atttactggc gaacgtccat ttgagaatgt cttgattcat ggattgattc gtgacgaaca
     2701 agggcggaag atgagtaaat cattaggtaa tggaatcgat ccgatggaag taatcgaaaa
     2761 gtatggagca gatgctctcc gctggttctt atcgaatggt tctgcaccag gacaagatgt
     2821 ccgcttcagc tatgaaaaaa tggatgcttc ttggaacttt atcaacaaaa tctggaatgc
     2881 ttcacgtttc gttatcatga atatcgaagg aatgaccgta gaagagatcg atttatctgg
     2941 agaaaaatca gtggctgacc ggtggatcct tactcgtttg aatgaaacga tcgaacgagt
     3001 aactgagcta tttgatcgtt ttgaatttgg tgaagcaggt cgacaactgt ataactttat
     3061 ctgggatgat ttttgtgact ggtacatcga aatgagtaaa gaagtacttt atggagatga
     3121 tgctgtttca aaacaaacca cccgtagcat cttagtccat acacttgatc agatcctacg
     3181 tttattgcat ccaatcatgc cattcgttac agaagaaata tgggaacata ttcctcatca
     3241 gggaaattct ctagtagtgg cagaatatcc tgttgtacgt ccagagtttg atgatgaaac
     3301 agcttctaaa gggatggaag tattgaaaga attgattcga gccgttcgaa atattcgttc
     3361 agaagtgaat acaccattgt ctaaaccaat tacgttgcta attaaaacaa acgatgctaa
     3421 aatcgatcaa tttttagttg aaaatacaag ctatatcgaa cgcttctgta atcccgaaga
     3481 gttgacgata tcaagtgaga tcactgcgcc agatctagcg atgtcagctg ttctgacagg
     3541 tgcagaaatc ttcttgccgt tagcaggatt gatcaatatt gaagaagaaa tcaaacgatt
     3601 agaaaaagaa ctggaaaagt ggacaagcga agtaaaacgt gtccaaggaa aactttctaa
     3661 tgaacgtttt gtttctaacg cgccggaaga agtagtagag gcagagcgtg caaaagaaaa
     3721 agattattta gagaagcaaa cggctgtcaa agaacgaatc gaaagtttgc gtactattca
     3781 ataagaatag aaaaagagag tgtgaaatca gctagctatt actaaaagct agctgattga
     3841 acatcgttac aggaaagagt ttgagcctca ctcttagctt cgattcataa ggagatgtga
     3901 cataaatttt gtcgcatctc ttttttagtc tactcttatt atgtaggaga aggagggagg
     3961 tggatcatgt atcaagagtc aataaagtag ttgataaaat cgttcctctc tctgtttctt
     4021 ttctctttgt catactttta tttttcttat gtgttctatt tgttataata aaagagaaat
     4081 gggggaattg ttgatgacga ttgaagaagc aatagaatgg atccatagtc gattaccatt
     4141 tggttcacgt cctggccttg accgagtcga agctttatta gaagtggtag ggcatccaga
     4201 gagaaaagta aaaacgatcc atatcgctgg aacaaacgga aaaggttcta ctgtagctta
     4261 tttgagaagc ctattagaag aggctgggct gacagtaggt acatttactt caccttatat
     4321 cgaaacgttc aatgaacgga tcgcaatcaa tggcaaaagc attccagaca atgatttgat
     4381 ccagcttgta aaaaaatatc agccactgat caaacaactt gatcaatttg aagcagtagc
     4441 tggtattact gaattcgaga cattaacagc aatggcattt gattattttt tgatgaagaa
     4501 agtggacgta gcgattatcg aagttggctt aggcggctta ttggattcta ccaatgtagc
     4561 tgtaccaatg ctgacgggaa taacaacgat tggacttgat cataccgaaa tacttggaga
     4621 tacaattgaa gaaatcgctg cacaaaaagc tggaatcatc aaacaaggcg ttccagtagt
     4681 aacaggaaat attgccccag aagcttttac tgtcatccaa caaacagcta aagcaaaaaa
     4741 tgcaccgctt tatgtatacg gggaaaatta taaaataaac tatctgcatc ctgatgagat
     4801 gtggggagaa gtatttgaat tttttaatga aataggaaag atccctcaat taaccactcc
     4861 gcttttaggg caacatcaag ctgaaaatgc aggagtagcc atcgaattgt attttgttta
     4921 ctgtcaattg gaaaagctgc ctttccgaga aaaagatgtt ttgaagggat tgaagaaatc
     4981 tcgatggcca ggaagaatgg agcgtttgag taacgagcca ttagttgttt tagatggtgc
     5041 ccataatacg catgcaatga accggcttgt tgagaatttg aaaaaagaat tcaaaggata
     5101 tcaaatcaac attttgtttt ccgccttacg aactaaaaat gttagagaaa tgctagaacg
     5161 attgcagcaa gtaccaaatg tccatattta tttgacgacc tttgaacatc caaaagccat
     5221 cgaattaagt gaagtatcag atttagcaag tgagagagta tcaatcgttt cattgtggca
     5281 attcggatta gcagatatat tggaaaagat gacaaatgaa gacgtattag ttgtgaccgg
     5341 ttcactttat ttcatttcag aagttcgcca tttattaata caattaggag gagatcatga
     5401 agtataatgc agtgattttt gacatggatg gtttgctgtt cgacacagag ttagtgtatt
     5461 acgaagccac acaagtagta gcagacaaga tgggttttcc ttatgataaa gacctgtatt
     5521 taaaattctt aggtgtttca gatgaagaag tatgggcgaa ttatcatatg attttctcgg
     5581 aatttggcaa aacaagtgta caaagattca tagatgattc ataccaggaa acgcttgaac
     5641 gtttttcgca aggagcggta caaatgaagc ccggtgtgtt ggaactcctc tcttttttag
     5701 aagaacatca gattcccaaa gttgtcgctt ccagtaacca acgagcagtc attgaacttt
     5761 tattgaagaa gaacaatctg tttgaacgat ttgagacgat tgtttctgca gaaaatgttc
     5821 aacgagcaaa accagaccca gaaattttct tgttagctaa tcagaagcta ggtacagcga
     5881 agaaagaaac attgatttta gaagattcac aaaatggtat tttagcagct tctgcggctg
     5941 agatcccggt agtcatggta ccagatctgc ttccaccatc ccaagaattg atcaaaaaaa
     6001 cagctggggt tgtttcttct ttacatgaaa tccctgcatt gttaaaataa aaatccatct
     6061 cttttcgtat cttattttag ataagataga aaggagatgt ttttttggga aaacgattga
     6121 tcaaagaagt acctactagc tctttaccaa gggaacgtat ggaaattatg gcgcggaagc
     6181 tttatcggat caggagcttt tggcaatctt actgcgtacc ggtcaacacc cttatagtgt
     6241 gatgtcgatt gctggaaatc tgttaaaaac gtttggagga ctggcctctt tacgacaggc
     6301 tactttgcat gagttagaag agattcgagg gataggacgc gtgaaagcaa tcgaaatcaa
     6361 agccttgatc gaattaggtc gacgaattca gacggatcat gaaagagcgg ctccaccggt
     6421 tcgtaccagc tacgaattgg cccagcaatt gattctggaa atgaaagatc acaaacaaga
     6481 acatttggtt tgtatttatt tggacacgaa gaatcaagtg atcttaaaaa aaacagtgtt
     6541 catcggttcg ctgaatcagt cagtcgctca tcctagagaa attttccatt atgctgtacg
     6601 ttattgtgca gcaaggatcg ttttagcgca taatcatcct tctggtaatg ttattccatc
     6661 gcagcaggat atgaatttta ccaaaaggat acaaaagtgt ggagaaatga tggggatcac
     6721 tgtactggac cacttgatca tcggacgcaa acgatatttt agtttgagag aagaaggaat
     6781 gatggaagag aagtaagttg ctttcttgca aaaaaaatga agcaagtgta caattatatt
     6841 gtaattttgt ttgggaatag aagttgaaac atctgttgca cctctatcgt acacgagcaa
     6901 tgttacaatt taaattagtg cttttggcac gaggaggata ttattatgga aaacggaaca
     6961 gtaaaatggt tcaacgctga aaaaggattt ggatttatct cacgtgaaga tggaagcgac
     7021 gtattcgttc acttctcagc tatccaaggt gacggattca aaacattaga agaaggtcaa
     7081 gcagtgactt tcgacgttga agaatcagac cgcggcccac aagctgcaaa cgttgtaaaa
     7141 gcttaatttt atctgtaaat caaattgatg gtagacatca atttttacga gagaacaaag
     7201 cgagtaacaa aaatacctgc cacaagagac aatttctctt gtggcaggta tttttttgta
     7261 tgaaaaagta aataaattta agaggctatg aaagagccac cttgtatcaa ggaataagaa
     7321 caaacaaaaa tggcttctca tgtttttcat ttgttttctc ttatattcag gacaaggtgc
     7381 tgatttcata acctcttatt tattcttcat ctggtgtcat taccattggc aaaatcattg
     7441 gatgtctttc tgtacgttcg aataagaaag gctgaagcgc gccagtcatc gcttctttca
     7501 atttatattc tgaacagtca ggtccattca aacattctct taaagcgtgg aataagattc
     7561 gctgagcttc attgatcatt tctcctgatt ctcgcatgta gacaaatcca cgagagagaa
     7621 tatctggacc agccaagatt tcttttttct tgatatccac tgtcgcgaca gctaatacaa
     7681 gaccttcttc tgaaagcagg tgacggtcac gtaaaacgac attaccaata tcaccgatcc
     7741 cgctaccatc gatatacaca tctccagcca caaaatgccc agcaggacgc gcagaatcgc
     7801 tagttaacgc taaaacatcg ccattttcca tgatgaagca gttttctgca ggaacaccta
     7861 catcttgtgc tagagaagca tgtactttca acatgcggaa ttctccgtgg acaggaataa
     7921 aatattttgg cttcatcaaa cgaagcatta atttttgttc ttcttgtcca ccgtgaccgg
     7981 aagtatggat gttattgatt tttccgtgaa tcacttctgc accagcttca gacagcaaat
     8041 tgatcaaatg attgacactt gttgtattac caggaatagg tgaacttgaa aagatcactg
     8101 tatcgtctgg ctggatttgg atttgacggt gagtaccatt cgcgatgcgg ctcaacgcgg
     8161 ccatcgcttc tccttgagaa cctgtacata ggatcatcgt ttcattggca ggcaagcgat
     8221 tgatttcgtg tgcatctaca aatgtgccgt ctggaacttt gatatatcct aaacgttgac
     8281 cgttgacgat ggcattttcc atgcttcgtc cgaaaacagc gattttacga ccagttgcca
     8341 cagcggcatc tgctgcttgc tgcaaacgga agatgttaga agcaaagctg gcaaaaatga
     8401 tacgtccttc gatttttgaa aagatcttca aaatagaatg cccaatcgtt ttttctgatt
     8461 tagtaaatgt tggaatctca gcatttgtac tatcagataa aaggcacaag actccttctt
     8521 ccccgatacg agccatttta tgcaagtttg ctggttcgcc aacaggagtg aaatcgaatt
     8581 tgaagtctcc agtcgctacg atatttccag aaggtgtttt caccacgatc cctaatgcat
     8641 ctgggatact atgggtcgtt ctgaaaaagc tgacagaagt ctttctaaaa cggatgatcg
     8701 tatcttcatt gatctcatgg agctctgcat ctcgaagcaa gccatgctca tctaatttgt
     8761 ttgtgatcaa cgctaatgcg agaggaccag catagatagg aatatttgct tgtttcagca
     8821 agtagggtat tccgccgatg tggtcttcgt gcccgtgagt aatcaccaag gcttttactt
     8881 tgtgtaagtt ttgtatgatg tagctgtagt cgggaatgac atagtcaatt ccaagtaaat
     8941 cgtcttccgg gaatttgatc ccggcatcaa tcaaaatgat ttcatcttgg aattgtaccc
     9001 catacgtatt tttcccgatt tcacctaatc caccaatagc aaagacgcct gtctcgttat
     9061 ttttgatgga aagtttcata ttaaaactcc actaatgaga aatcagcatt ttctttttcg
     9121 tactctagat gacttccttc tagaagttga acgaattcaa tattgtatgg ggtattttct
     9181 tccacacgtt ggcgagcgtc tacatcgctt tttgcctcaa tgtaaagaga ttttgtttct
     9241 tcccgtttag ggttgcgtac ttttgtttct tgataataaa ctttatagat cattgttttg
     9301 tctcctttat ctgcgtaaag cagtgattta gctagttgat ttttttagac aacacaaaac
     9361 gaggggacaa gccacctggt ttttcatatg ctttgactag aatactggat tcgagtcaaa
     9421 gagacaagcc tcgtttttgc ttatctgttc ggatattttt tcaaccgtac cttctgtata
     9481 aaaaatatac aacaattaat tgttattcta gcataaaaca gcaaataagt atagaaatca
     9541 actagattga aagaaaaaat caatgtttat catcccgcta taatcataat gactttttta
     9601 tgagagaagt gttctattaa taggctcgtt gtgctatcat ttttagtaat gaaaatgtaa
     9661 caaaagatta aaaggagatg agaaagtgaa actaatgtgg cattatacca tgcgttataa
     9721 gaaattcttg tttttcaatt tcatatgcgt atttggtttt attttgattg aattaggact
     9781 tcctacgatt ttagcacgta tgatcgatgt aggaatccgc aatgatgatt ttgattatgt
     9841 gaagcaacaa gggatattta tgatcgttat tactgtaatc ggtattattt tgaatatctt
     9901 gttaggttat tttggttcga ggattacgac taatatcgta gcagatattc gagatgattt
     9961 gttcgaaaag atccaaagtt attcgcatca ggaatacgaa aatctaggcg tatcttcatt
    10021 gatcactcgt acaacaaacg atgcttatca gatcatgttg ttcctgcaaa atattttacg
    10081 tatcggtttt atggcaccaa tgatgttcat tgtcagtttg tacatggtca tgcgtacaag
    10141 tgtgacactc agtttgtatg tagtaggtgc attaccttta ctattattgg cagtcgtagc
    10201 gattgctaag gtgtctgaac cgttatctaa aaagcaacaa aaaaacttag ataaaatcaa
    10261 cagtatcttg cgggaaaatc tttcaggatt gcgtgtgatt cgtgcattcg tcaatgaaaa
    10321 gtttgaagaa aaacgttttg ctgtagtcaa tgagaattat gcttctagtt caaaaagctt
    10381 attccgtttg atggctgttg cacaaccggg tttttatttc ttatttaata tcgtaatggt
    10441 attgatcatc tggagcgggg cagtgcaaat cgatgcaggc gagcttcgtg taggagattt
    10501 gattgcgttt atcgagtata tattccatgc cttgttctca ttcatgttat ttgctagtgt
    10561 gttcatgatg tatccaagag cagctgtttc agctggacgt atccaagaag ccctagatgc
    10621 caaaccttcg attgtagaag atccaaatgg tatatctgat acaaaaacta aagggtatat
    10681 cgaatttcaa aatgtaacat ttgcttatcc aggccattcg caagagccag tgatacgaaa
    10741 tgtcagtttt acagcctctc caggagagac cgttgctttt attggaagta ccggtagcgg
    10801 aaaatctacg ttgatccaat tgatccctcg cttttatgat gtgagtgaag gaaagatctt
    10861 gatcgatgga gtagacgtga gagattatcg gctaagcaag ttgcgagata agattgggtt
    10921 tattccgcaa aaagcgctgc tatttactgg aacgattgca gataatttgc gatacgggaa
    10981 agaagatgct acgcaagaag aaatggaacg tgcagctgag attgctcaag cttcagaatt
    11041 catttctcga aaaccagatg gatttaatga acatttatca gaaggtggtt ccaatttttc
    11101 aggtggacaa aaacaacgat tggcaatagc cagagccatc atacgccgtc cggaaatcta
    11161 tatttttgat gacagctttt ctgcattaga ttatcaaacg gatgcgaagt tacgtgctcg
    11221 tctgaaaaaa gaaacgacag aatctactgt attgatcgta gcacaacgtg tgggaacgat
    11281 catgcatgca gataagatca tcgtattaaa tgaaggagaa gtcgtcggta tggggacgca
    11341 tcgggagttg ttggaaaaat gcccgattta ttatgatatt gccgcttctc aattatcaaa
    11401 ggaggaatta gcatgaaaaa aaccttttct tccttaaaac gtttagctcg atatatcaga
    11461 ccgtatcgtg agacttttct tttagtgatc ctatttacag ttctgacagt ggctttcaac
    11521 gtggctttgc catatgttac aggacttcct actacagaaa tcagccgtaa tattgccaat
    11581 ggcgaatcaa tcaatttttc ttatatattc aaatgtctca tttggatcac agttgttgga
    11641 atagggtatt gtatttcgca attactttct ggtgttttga tgacaaatgt cgttcaaagt
    11701 gccatgcatg atttgagaag agacatagaa gaaaaaatca atcgtcttcc ggtttcatat
    11761 ttcgataaaa accagcaagg aaatattttg tctcgtgtga cgaatgatgt agatgcagtc
    11821 agcggtgctt tacaacaagc ttttattggt atcgtaaatg cgatattggg aattacgttg
    11881 gctgcagcaa tgatgtttta tatccagccg atgatggcgc tgatctctat gatcatgatt
    11941 ccaacatcga tctggatctc aaaacgagtg atcaatgctt cgcagaaata ttttcaaagc
    12001 atgcagaatt ctcttggtga gctaaacggc tacgtccaag aaaatatgac aggttttagt
    12061 gtattgaaag tatatggacg cgaaaaagaa acattggaag gattcaaaaa agttaatcac
    12121 agcttgaaat actttgggtt tcgtgcagca ttcatttctg gcttgatgat gcctctagtt
    12181 caattaactg cctatgctac ttatattggt atggctgttc taggaagctt ttatgttatt
    12241 actggggcga tcgttgtcgg tcaactacaa gcattcatcc aatatatttg gcaaatcagt
    12301 cagccaatgg ggaacgtgac acaattgtct tcagttttgc aaagtgcatc agcggcaacg
    12361 aaacgtgtat tcgagatttt agatgagcca gaagaaaaag aaaacaaagt agatgcttca
    12421 ctgccagaaa cgattttagg agatgtgaca ttcgatcatg tcgattttgc atatgatccg
    12481 aagaaaccat taatccaaga tttgaatttt gaagtgaaag ctggtcaaac cgtagctgtg
    12541 gtcggtccga ccggtgccgg caagacgaca ttgatcaatc tattgatgcg tttttatgac
    12601 gtagacaaag gagcaataag gattgatgga atcgacacca aatcaatgag tcgcagtgat
    12661 gtgcgttcgc tatttggtat ggtcttgcaa gatgcatggc tttatcaagg aacgattgca
    12721 gataatatcc gcttcggaaa gcttgatgcg acagattatg aagtagttga tgctgcgaaa
    12781 acagcaaacg tagatcattt tatccgaacg atgccagatg gatatgatat ggccatcaat
    12841 tctgagggag acaatgtgtc tttaggacaa aaacagttgc tgacgattgc ccgagcagtg
    12901 gtctccgatc ctaaaatctt gattttagat gaagcaacaa gttcagtaga cacccgttta
    12961 gaagcgttga ttcaaaaagc aatggatcgc gtgatggagg gacggacaag tttcgtcatc
    13021 gctcaccgtt tatcgacaat ccgtgatgca gatctgattc tagtgatgga tcaaggtcag
    13081 atcattgaaa aaggaacgca tgagagttta ttggcacaag gtggatttta tgaaaaactg
    13141 tataacagcc aatttgctga ggaagcagaa taagtcatat aagaataaaa cagtttaaaa
    13201 cttatttttt gttctctatt cttgataatg gtaaagtaaa gttatcaaga aaagtgagga
    13261 cacggagatg gaacagtttt taaatgaaaa aattactgaa gccaaaagag aagttgatcg
    13321 tttaacgcac gtctgtcaga cgaagccttt tgattcagct gatttttttg aaaatgagtt
    13381 tcaaatcgaa cgcttggcag cacaaataga agtgtacgaa ttaattttaa aaaaagtaat
    13441 agaagaaaat atagatgtac aataaaaaca ggtaaaacgt cagttaccag atactgtaaa
    13501 tagacatctg gcctgagcag gttttacctg ttttttttct tatgaaataa atatatgcta
    13561 tgataccttt attcaatagg attagtttct acaaaaaatg aaaggagaaa aataaatatg
    13621 gaacggttta tagcatcaaa tgccacagtg attggtgatg tgacattgtc agaagatgtg
    13681 acgatctggt atcaagctgt tttacgcggg gacagtaatt ggataaaaat aggacaaaga
    13741 acgaatatcc aagatggtac aatcatccat gtcgatcatg atgcacctgt agatattgcg
    13801 gagaatgtga cggtaggaca tcagtgcatg ctacatggct gtacaatcga aaaaggggcg
    13861 ctgatcggga tggggaccac gatattgaat catgcggtta ttggtgagaa tagtttgatt
    13921 ggtgctggat cattagtaac agaaggaaaa gttattcctc ctaatgtctt ggcttttggt
    13981 cgcccagcta aagtgatccg tcctttgaca aaagaagaaa tacaaaaaaa caaagaaaac
    14041 atccagcatt atgtcgagat aggtcggatg catgcgcaag gaaaattcaa ggagcaaaga
    14101 taggcagtag catccataaa ataaaaaagg atggtcagtt tgagttgacc atcctttttt
    14161 atttggttta gataagagag cttaaaaaag cttcctttga aacagattaa tttaatgaag
    14221 aactgttttc catgacttca tcgatcaatc catatgcttt tgcttgttca gctgtcatat
    14281 agttgtcacg atctgtatct ttttcaatca cttcaagcgg ttgtccagtg cgttcagcta
    14341 aaattttgtt taagcgttcg cgtgtttgca aaatatggcg tgccgcaatt tcgatttcag
    14401 ttgcttgccc ttgagcgccg ccaagaggtt gatggatcat gatctcagca tttggtaaag
    14461 caaatcgttt accttttgta ccagctgtca gcaagaagct tcccattgaa gcagccatac
    14521 ccataacaat tgtttggaca tctgctttga caaagttcat tgtgtcgtaa atagccatac
    14581 ctgctgtaac tgaaccacca ggagaattga tataaagata gatatctttt tcagaatctt
    14641 gagcgtctaa gaaaagcaat tgggcgatga tcgaattagc aagatcatct gttacttggc
    14701 cgcttaacat gatgatacgg tctttcaata aacgtgagta gatatcgtaa gctctttcac
    14761 ctcgtgaaga ttgttcaatg actgtaggaa ttaaattcat aatagagttc ctccttgatt
    14821 tcgtttagaa atagtgtacc ataatttagt ttctaatacc taaaatgtag tatacatttt
    14881 tggtcaaaaa aggtcaaata aaaaacttag gcaaattagt cgcataaacc cgctttatgg
    14941 tatgataagc agagaaacaa aacaaggagg aacaacagat tgatagttgg tattactggc
    15001 cttatcggag tgctgacagg tgctgtagtt gtctatatcg gcatgatact gcaaatgcgt
    15061 cacttggaaa ataaagaaca gcgtagacgt gaattggaat tgaaagtcaa agaaatcgag
    15121 acgctgaatc aattaaataa aaagatcaac gagatcttgc aaaaacggac aacgctgatg
    15181 gaagaatatg tcagctttga tgcatttgat gattgttata tcacgattga cgattttgcg
    15241 tatctccaat cattttcagc gcaaaatagt ttttatctgc caaattatat tttagacgag
    15301 ctatttaaga atatctccca tcgaaaagtg attttatctc cagatgaaac aatgaagatt
    15361 ggtggatatg cttataaagg cggacgcgtg attttagaga attttacgga tagtctgact
    15421 gaaatgatca atgaaaaaaa agctcagttg aagactatga cgcgccagcc gattggtttc
    15481 ttctcgaaag atgatgtttg gagctgatat tttgaagcat tgccgttgca catgagagcg
    15541 tggtaatgct ttcttttttt cttaatgata caatagaact gaacggagtg aagaaagatg
    15601 ttacaaatct atgcccatcg cggaagcagc ggcacccacc cagaaaatac gcttcctgca
    15661 tttgcagaag ctgtacgagt aggtgcagat ggtatcgaac tagatgtcca tctaagcaaa
    15721 gatggttatt tgatcgttat gcacgacgaa gaagtggacc ggacgactaa cggtaaaggg
    15781 ctgattcgag aaaaaacatt ggaagaactc aaaaaattga atgcaggggg caaattcagc
    15841 aaagaatttc cagcagctaa agttcctgaa ttgagagaag tgttaggact gctcctccaa
    15901 aaaaattacc gagggatgct aatgattgaa attaaaactg atcagtatga atatcccggt
    15961 atcgaagaaa aattagccga aacattaaaa agtagagatt ggccttttca acatgcatat
    16021 tgcagctttc atttggattc tttggaaaaa atcgcattac tggaaccgga gatacagctg
    16081 gacttattga tgagtacttc tgataaaaag atccaactag cagcagaaac cccttatata
    16141 gaaggagtac atccaaaaaa tacttggttg ttcgaacatc cagtccaggc agctgatttt
    16201 ccaaaagcaa ttcgtgcatg gacgattaac gatgaagaag aaatggccaa atgtcaagaa
    16261 ctcaaaatag atggaataat aactgatttt ccggagaaag ctcttcggtg gaaacaatca
    16321 acgaaaagta ggtaaaatat ggaaaaagta ttagtgatcg tcggtccgac agcagtcggt
    16381 aaaacagcgc taagtgtgga actcgcacaa ctatttggag gagagatcat cagtggtgat
    16441 tcattgcaaa tctataaaaa tctggatatc ggtacagcaa aaatcaaaga agaagagatg
    16501 aataatgttc cgcatcattt gattgatgtg atcgaaccta ctcaacgtta ctcagccgtt
    16561 gattttcagc attcaggtcg acaattgatt accgagattg cgaaccgagg gcacctacct
    16621 atcatagctg gtggtacggg tctctatatc caatcccttc tatatgatta tcagttagga
    16681 gcgacagaag aagcgaatcc tgagattaga aaaaagtatg aaacgttagc tgaaacaatt
    16741 ggttcccaag cattatggaa tcagttgaaa gaaaaagacc cggcagctgc tgagaaaatc
    16801 cactggaaca atcaacgcaa agtgataaga gctctggaag ttttagagac gacaggacat
    16861 agtatcacgg cacctaaaga agagccaaag caattgtatg attacctcat gattggatta
    16921 gatacgaagc gtagtattct ttatgaacga atcaatcaaa gagtcgattt gatgctagca
    16981 gaaggtttag tagcagaagc gcgtaaggtg tatgagcttg gagaagtcca ggccagtcaa
    17041 ggtattggat ataaagaatt tttcccttat tttgaaggga aagaaacatt ggaagaagca
    17101 acagaacaag tcaaactaca ttcgagaaga tacgcgaaac gtcagttgac gtggtttcgt
    17161 aaccgtttgg atgcacagtg gtttgatctt ataagtgagc caaatcaaaa aagtcagatt
    17221 gagcaaacaa tcaaaaaatg gctggaggcg gaataaaatg gaagaaaaag tgatcatcgt
    17281 aggggtagaa accgaagcaa accagcgtta cttttcagag tcaatggaag agctagttca
    17341 gctgacgaat accgcatcag gagaagtcgt attcacaatt acacaaaaga gaccacaagt
    17401 ggatcggcaa acgattatcg gtaaagggaa attacaagaa ttagttcaac aagcggatgc
    17461 gcatgaagcg gatttgatca tctttaatca tgagctaact ccgagacaga gccaactgat
    17521 taccgatgct gttggtcttc cggtaattga tcgcgtgcag cttatcttag atatttttgc
    17581 gatgcgtgct cgttcgaaag aagggaagct gcaggttgaa cttgctcagt tagaatacct
    17641 gctgcctcgt ttagtagggc aagggaaaac attatctcgt cttggcggtg ggatcggaac
    17701 aagagggcca ggtgaaacaa agttagaaac agaccgccga catatacgga ataaaatttt
    17761 tgctgttaag aaagaattga aagaagtaga agctcatcga gagcgcaatc gccaaaaaag
    17821 aaaaaatagc gagatttttc agattggact tattggctac acgaatgccg ggaagtcaac
    17881 tatcttgaat ctattgacac aagcggatac ttattcgaaa gatcaattgt ttgcaacgtt
    17941 agatccattg actaagagat ggcgttttgc tgaaggattt gagattactg taacggatac
    18001 cgtcgggttc atccaagacc tgcctacgca actgatcgat gctttccatt ctacattaga
    18061 agaaagtcaa aatatggatc ttttgctaca tgtagtcgat gctagttctc cagatcgtat
    18121 tttacaggag cagactgtct taaaattgat ggatgaactg aatatgaaag aaatgccgat
    18181 actgactgtt tacaataagg cagaccagat cgacccagcg atgttcacgc ctacgctatt
    18241 cccaaatgta ttgatttcta ctcagacgaa agaaggtaag atggcgttga tcgaagcaat
    18301 caaacgtcag ctgatggaac taatgacccc ttatacactt tctgtgccaa gcaacgaagg
    18361 acagaaatta agcgagttga gaagacaaac aatggttttg gacgaagcgt acatggaaga
    18421 aacaaacgaa tatgaagtta aagggtttgc aaaaaccaat tcaaaatggt taagaaaaaa
    18481 ccaataaaat tttatttttt taa
//
LOCUS       DACGLE010000054        17962 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0534
            SAMEA3304449-rid9560453.denovo.058, whole genome shotgun sequence.
ACCESSION   DACGLE010000054 DACGLE010000000
VERSION     DACGLE010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304449
            Sequence Read Archive: ERR985064
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17962)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17962)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702225.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702225.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 20:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,045
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 27 of 67
            Pseudo Genes (incomplete)         :: 25 of 67
            Pseudo Genes (internal stop)      :: 19 of 67
            Pseudo Genes (multiple problems)  :: 4 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17962
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304449-rid9560453.denovo.058"
                     /strain="VREN0534"
                     /db_xref="taxon:1352"
     gene            complement(27..1523)
                     /gene="lysS"
                     /locus_tag="IXT20_001945"
     CDS             complement(27..1523)
                     /gene="lysS"
                     /locus_tag="IXT20_001945"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007208013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HAQ4860383.1"
                     /translation="MTQEQQHQVEDLNDQMLVRRQKMEQLREDGIDPFGKRFERTHNS
                     EELHELFDPRTKEELAEMGLTASVAGRMMTRRGKGKTGFAHLQDREGQIQIYVRKDQV
                     GDEAYEVFKHADLGDFFGVTGQIMKTDTGEVSIKASEITILSKALRPLPDKYHGLTNI
                     EQRYRQRYLDLISNRESFDRFMKRSQIISEIRRYLDGNGYIEVETPVLHNEAGGAAAR
                     PFITHHNALDIDLYLRIALELHLKRLIVGGMEKVYEIGRVFRNEGIDTTHNPEFTMLE
                     AYTAYTDFNDVMNLTEGIIRNAAEKVLGTAKITYDGQAVDLESDFKRIHMVDAIKEQT
                     GVDFWQEMTLEEALALAEKHNVEVTEAMGVGHVINEFFETFVEETLTQPTFVYGHPVE
                     VSPLAKKNPEDPRFTDRFELFIVGREFANAFTELNDPIDQRERFEAQEKERELGNDEA
                     HGVDEDFLEALEYGMPPTGGLGIGIDRLVMLLTDAQSIRDVLLFPTMR"
     gene            complement(1634..2638)
                     /gene="dusB"
                     /locus_tag="IXT20_001946"
     CDS             complement(1634..2638)
                     /gene="dusB"
                     /locus_tag="IXT20_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HAQ4860384.1"
                     /translation="MEHTWKIGNVEIPNRVVVAPMAGITNSAFRVTVKEFGAGLVVCE
                     MISDQGIHFRNKKTLEMLHIEETEHPLSLQIFGGNKDSLVEAAQFVEENTAADIIDIN
                     IGCPVNKVIKAEAGAKWLLDPEKVYEMVQAVSSAVNIPVTIKMRIGWDEEHVFAVENA
                     LAAQSAGASAIAMHGRTRVQMYEGKADWEVLKEVKKHLTIPFMGNGDVKTPEDAKWML
                     DYVGADGVMIGRAALGNPWMIHRTQHYLETGELIPEPTPAEKIATAKLHLQRLADLKG
                     EKVACREFRTHAAYYLKGIPRAAKVKVAINQTEKQQEINDLLDQFVEKAEKVLLKQTL
                     "
     gene            complement(2639..3526)
                     /gene="hslO"
                     /locus_tag="IXT20_001947"
     CDS             complement(2639..3526)
                     /gene="hslO"
                     /locus_tag="IXT20_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HAQ4860385.1"
                     /translation="MKDYLVKALAHDGFVRAYAVQATNTVAEAQRRHDTWHTSSAALG
                     RTLVGSLLLGATLKGEDKLTVRIQGDGPATGIVVDANGKGDVKGYIKNPHLSLPLNSE
                     GKIDVRGAVGTQGMFTVTKDLGLKEPFSGQTPIVSGEIAEDFTYYLAISEQIPSAVGL
                     SVLVNTDDSIKTAGGFMIQIMPGADEETISKIEEQLKTTPRISTLLDEGQTPEEILKG
                     LLGTEELNILEKMPVQFKCDCSKEKFAEAIIALGAEEIQAMIDEDHGAEAVCSFCNNK
                     YKYSEEELDGLKKEALGGI"
     gene            complement(3743..5854)
                     /gene="hflB"
                     /locus_tag="IXT20_001948"
     CDS             complement(3743..5854)
                     /gene="hflB"
                     /locus_tag="IXT20_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HAQ4860386.1"
                     /translation="MNKKNNGMMKNALYYVLVILAMVMVVYFIFGNNGQRSSDIEYSK
                     FTEQLQAGKIKEFEVQPANGVYKITGEYKEQQTVANSGGLAILGSTDTTTTHFTTIIL
                     PNDTTLSQVTELAKENKVATTVKEESSSGIWVSLLLSFLPIVIIIFFFYMMMGQQGGG
                     GGGGGRVMNFGKSKAKEADKKANRVRFSDVAGAEEEKQELVEVVEFLKDPRRFVELGA
                     RIPAGVLLEGPPGTGKTLLAKAVAGEAGVPFYSISGSDFVEMFVGVGASRVRDLFETA
                     KKNAPAIIFIDEIDAVGRQRGAGMGGGHDEREQTLNQLLVEMDGFDGNEGVIVIAATN
                     RSDVLDPALLRPGRFDRQILVGRPDVKGREAILRVHARNKPMADDVDLKVVAQQTPGF
                     AGADLENVLNEAALVAARRNKKKIDASDIDEAEDRVIAGPAKKDRVISKKEREMVAYH
                     EAGHTIVGLVLSRARVVHKVTIIPRGRAGGYMIALPKEDQFLMTKEDMFEQIVGLLGG
                     RTAEEIIFNVQSTGASNDFEQATALARSMVTEYGMSDRLGPVQYEGNHQVFVGRDYGQ
                     TKAYSEQVAFEIDQEVRKILMEAHDKAREIIEAHRAQHKLIAEKLLEFETLDAKAIKS
                     LFEHGVMPEGTESNDYPSEKEAKTFEEAKRALEEKDAEKQAEEKRDFEEAKHELNEEN
                     KAEHDSDSQQSEDNHSDDQYK"
     gene            complement(5944..6489)
                     /gene="hpt"
                     /locus_tag="IXT20_001949"
     CDS             complement(5944..6489)
                     /gene="hpt"
                     /locus_tag="IXT20_001949"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HAQ4860387.1"
                     /translation="MLEKDIERILISQEEIQVRCKELGKELTEIYQNTNPLVVGVLKG
                     AVPFMADIVRSIDTYLELDFMDVSSYGNATVSSGEVKIVKDLDTNVEGRDLLIVEDII
                     DSGRTLAYLVDLFKYRKAKSVKIVTLLDKPEGRVVNIEADYVGFNVPNEFVVGYGLDY
                     AEAYRNLPYIGVLKPSVYQSN"
     gene            complement(6489..7934)
                     /gene="tilS"
                     /locus_tag="IXT20_001950"
     CDS             complement(6489..7934)
                     /gene="tilS"
                     /locus_tag="IXT20_001950"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HAQ4860388.1"
                     /translation="MNVEKGGKKAPLLQQSFIRHGNEQGFWQQGSRILAAVSGGVDSM
                     VLLHLLLQVQRQIGFSLGIVHINHQLREESAEEAAYLRSYCEEKNLPLYVSIWEDPAK
                     SAIETEARNFRYQEFTRVMEEGQYDTLMTAHHGDDQIETVLMKIVRGGQLNTFSGIKE
                     VQPFATGRLIRPLLSFSKEDLYTYAAESQLVYFEDQTNQLLDIQRNRLRHLVVPQLKQ
                     ENTQALRHFQQFSQQIQWADQVIQKYMGQLIEKEVEQWEEQFRFSAEMIEEMTDAERY
                     YFFYSFFDKAFSKTGIILKEQQMESILDQWQQKKSQWQLSIGNNWILKREYHFVVLSK
                     KEKQATSQTEDQRLLLIPEQGIYLNETEWIGLVKPENAGDIKATDSWSLFSHDIWIPA
                     DVQLYVEKRKAGDRIKLTENLTKKVSRYFIDQKVPNSQRQHAWIITDAKRNIYSLIPF
                     AFSYLSIREETDKIHYILLYKYQKEAIGRRT"
     gene            complement(8054..8524)
                     /locus_tag="IXT20_001951"
     CDS             complement(8054..8524)
                     /locus_tag="IXT20_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein S1"
                     /protein_id="HAQ4860389.1"
                     /translation="MSIEVGAKLQGKVSGITNFGAFIDLGAGKTGLVHISEVSNGYVK
                     DIHDVLSVGDEVTVKVISVNDDGKIGLSIRKAQEQPAESGEESRPKKYQQKDPRDNRN
                     RPAQKKSFSRSQPSAQKEDFDSLMSSFLKDSDDRLSSLKRNTEGKRGGRGGRRS"
     gene            complement(8570..8995)
                     /locus_tag="IXT20_001952"
     CDS             complement(8570..8995)
                     /locus_tag="IXT20_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HAQ4860390.1"
                     /translation="MTKKSNKIAALDTEYAKKKYAEFQQQQRQLIFRRRRLAAIFVGA
                     LVIFAIIGVQIFHDMQRMHQLNELRVEANTEMKEVNADVDQLHQDVSLLKDDEYVAKL
                     ARSKFFYSKDGEKVYPILESNTDSSTNETDQSTSSTTAK"
     gene            complement(9062..9331)
                     /locus_tag="IXT20_001953"
     CDS             complement(9062..9331)
                     /locus_tag="IXT20_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HAQ4860391.1"
                     /translation="MRLDKFLKISRIIKRRSVAKEVADKGRIQINGKLAKSSSDVKVG
                     DILKVQFGNKTLEVKITELHESTKKEDAQKMYEIISENRTETLEK"
     gene            complement(9342..10937)
                     /locus_tag="IXT20_001954"
     CDS             complement(9342..10937)
                     /locus_tag="IXT20_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAQ4860392.1"
                     /translation="MDHKEMRRVMQGAFVLTIASFIAKVLSALYRIPLQNLVGDEGFY
                     VYQQVYPIYGIAMTLALSGLPQFISKYVAERKNPFERKQALQNLYPLVFWTGVFLWLF
                     VFAFSSWIAWMMGDIQLKPLIQVVSFTFLLMPGLSFYRGNFQGRFLMEPTAVSQVVEQ
                     FVRVFVIVCSAFAFRKLGWTVYQTGTAAMSGAVFGGLCAYGILHYYEQKIHGGALSFR
                     RFPLFKQPSKPLIRRFLVEGGLVSIYSGLLIFFQLIDSFFVKNALEVYGLSEYGAKIA
                     KGVYDRGQPLVQLGLVIATALSATFLPALTRHLTNRIYRQFLQTAKIYLRLTTALALA
                     ASLGLALLLPYINYALFKDYAGNAALVLFVFSIAFTAVIQAYQSIAQSKNSFRPSLKG
                     AGWGLLVKGLTTSFLTGLLGTAGASLSTLLGLGVTLWYFVRIETKELNAFWKERHFGR
                     KLMFSLLCMTVVLLLYYGVLNIVFGPVTHRSTAFFVSVIGVGIGASVFIYAVIRLRLF
                     TVREWLMLPFGKKILRLNKKIKK"
     gene            complement(10952..14473)
                     /gene="mfd"
                     /locus_tag="IXT20_001955"
     CDS             complement(10952..14473)
                     /gene="mfd"
                     /locus_tag="IXT20_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HAQ4860393.1"
                     /translation="MDIIQLVGKDPQVKEWVSNLEEKMPRQLITGLAGSAKTLLFARA
                     FKKTNKNMIILMPNLYYANQLAEDLQHVIPQDQIHLFPVDEVLSAEMAFSSPEARGER
                     VATLNALQEKKAGIYLLPVASLHKRLPNKKTWLQAQLNWHIGDEIDVENLPRQLTLMG
                     YVREEMVAKPGEFSIRGSIIDIYPLTTTYPVRVELFDVEIDSMRYFEAETQRSIEKTE
                     EIWVSPTSEQVYGPEDLEHGIEQMQKMLEKRLAATTEEADREFLQDYFGQLTSSWQAG
                     VPTEEAKFYSDLLYQESVTILDYFPSNSLLVVDDYQRIMETNREIEREAAEWQTQKIS
                     ELRVFSEQTFSADIQKIVQKEKFTTTFFSLFQKGMGNLRFQAIHNFQYRTMQQFFGQM
                     PLLKTEMDRWRKQKQTVVIFIPTKDRIRKAEQMLRDEDILVVETEKNALIRGQVQLVE
                     GALQAGFELPQEKIVAITEKEIFQKTTKKQTRRQTVTNAERLKSYNELKAGDYVVHAN
                     HGIGKYIGMETLEVDGVHQDYMTILYQNDDKLFIPVTQLNLIQKYVASEAKAPRINKL
                     GGSEWTKTKRKVSSKIEDIADDLIKLYAARESEKGYAFGPDDAYQKEFENAFPYSETD
                     DQLRSAAEIKRDMEKEKPMDRLLVGDVGYGKTEVALRAAFKAIKESKQVAFLVPTTIL
                     AQQHYETMLERFEGFPVNIGLLSRFRTRKQQKETIEQLRTGQVDIVVGTHRILSKDIE
                     FSDLGLLIIDEEQRFGVKHKERLKQLRAQVDVLTLTATPIPRTLHMSMLGVRDLSVIE
                     TPPANRYPIQTYVMEKNPGAIREAIHREMGRGGQVFYLYNRVETIEQKVEEIQELVPE
                     ARIGYAHGQMTEAQLENTLFEFIEGQYDVLVTTTIIETGVDIPNANTLFVENADYMGL
                     STLYQLRGRVGRSSRVAYAYFMYEQQKILNEVSEKRLQAIKDFTELGSGFKIAMRDLS
                     IRGAGNLLGAQQHGFIDAVGFDMYSQMLSEAVSRKQGKNSQVEKTTVEIDLGVDAYLP
                     ETYVADQRQKIEIYKRIRELDSQEMLDELEDDLLDRFGEYPEEVAHLLTIGQIKMDGD
                     RALLETIRKQEAHIVFTLSKVGTKRYSVEQLFEALTATKLKASLGVEKEQMVIRLTIP
                     NQMEEAVWLQEIQKFVKALREQKYLSA"
     gene            complement(14490..15050)
                     /locus_tag="IXT20_001956"
     CDS             complement(14490..15050)
                     /locus_tag="IXT20_001956"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HAQ4860394.1"
                     /translation="MKMIVGLGNPGTKYQYTKHNIGFMVVDKIAREHQATFKKNPFEA
                     EVAEFFHNGEKILLVKPQTFMNESGRAVGPLMTYFGIYPEELVVIYDDLDLAVGKIRL
                     RQKGSAGGHNGIKSIISHLNTNVFDRIKVGIGRPEGKKTVVQHVLSPFSKENQPLIEE
                     SMCQSVKAVEYLIEGHSFVDAMNRFN"
     gene            15403..16377
                     /locus_tag="IXT20_001957"
     CDS             15403..16377
                     /locus_tag="IXT20_001957"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HAQ4860395.1"
                     /translation="MTAAAEKKDHQKVILVGDGAVGSSYAFALVTQNIAQEVGIVDIN
                     TAKTEGDAIDLSHALAFTSPKKIYSATYADAHDADLVVITAGAPQKPGETRLDLVNKN
                     LKINRDVVTQIVESGFNGIFLVAANPVDILTYSTWKFSGFPKERVIGSGTSLDSARFR
                     QALAELVDVDARNVHAYILGEHGDSEFPVWSHANVAGLQIYEWVKNNPEIDEEAMVNL
                     FFGVRDAAYTIIEKKGATFYGIAVALARITRAILDDENAVLPLSVFMNGEYGLNDIYI
                     GAPAVINRQGIQKVIEIPLSDSEQDRMAASAKQLKEILDEAFAKLDAE"
     gene            16547..17947
                     /locus_tag="IXT20_001958"
     CDS             16547..17947
                     /locus_tag="IXT20_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HAQ4860396.1"
                     /translation="MTRSSHRKKSKHVALLSVLGVLLVLISFYTYRSTYYTKHFLPNT
                     EINGINVSNLTVEKANEKLKEAYSDKKLAIKEDGKVWQEVPKSELGYKDDFTSELSPI
                     LNEQNGWTWGMTYVSAAEKQEIDPVSQNHQKLDTTVQTLTTKLTDLNKDRTPTTDATI
                     EKAGDSFVIKPEVNGNTIDVDAAVKQLKSAVNSGKDTIELTEFKEKPKVTSEDSSLKE
                     QLASMNAIANVKATYSINGETFQIPSSDIMSWLTYNDGKVDLDTEQVRQYVTDLGTKY
                     NTSTNDTKFKSTKRGEVTVPVGTYSWTIQTDSETEALKKAILAGQDFTRSPIVQGGTT
                     ADHPLIEDTYIEVDLENQHMWYYKDGKVALETDIVSGKPTTPTPAGVFYVWNKEEDAT
                     LKGTNDDGTPYESPVNYWMPIDWTGVGIHDSDWQPEYGGDLWKTRGSHGCINTPPSVM
                     KELFGMVEKGTPVLVF"
BASE COUNT         4880 a         3970 c         3240 g         5872 t
ORIGIN      
        1 gaattcctta gacagctttt attcgattat ctcattgttg ggaatagcaa aacatcccga
       61 attgattgtg catcagttag caacattact aatctgtcga tcccgatgcc tagaccacca
      121 gttggcggca taccgtattc taaggcttcc aagaagtctt cgtctacacc gtgagcttcg
      181 tcatttccta gttcacgttc tttttcttgc gcttcgaatc tttctctttg atcaattgga
      241 tcgttcagtt ctgtaaaggc attagcaaat tcgcgcccca ctatgaatag ttcgaatcga
      301 tctgtgaatc gaggatcttc tggatttttc ttcgctaaag gcgaaacttc gactggatgt
      361 ccgtatacaa acgtcggttg tgtcaatgtt tcttcaacaa atgtttcaaa gaattcattg
      421 ataacatgtc cgacacccat agcttcggtt acttctacat tgtgtttttc ggctaaagct
      481 aatgcttctt ccaatgtcat ttcttgccag aagtctacac cagtttgttc tttgattgca
      541 tctaccatat ggatgcgttt aaagtcactt tctaagtcga cagcttgtcc atcatatgtg
      601 atttttgctg ttcctagtac tttttcagct gcgttacgga taataccttc tgtcagattc
      661 attacatcat tgaaatcagt atatgctgtg tatgcttcta acatcgtaaa ttctggatta
      721 tgtgtagtat caattccttc attacggaaa acgcggccga tttcataaac tttttccatt
      781 ccgccaacaa ttagtcgttt taaatgaagt tctagcgcaa tacgcaaata taaatcaatg
      841 tccagtgcat tatgatgcgt gataaatggg cgtgcggcag ctccacctgc ctcattgtgc
      901 aatactggtg tttctacttc aatatagcca ttgccatcta aatagcgacg gatttcgctg
      961 ataatttggc ttcgtttcat aaatcggtca aagctttcgc gattactgat taaatccaag
     1021 tagcgttgtc tgtagcgttg ttcaatattc gttaagccat ggtatttatc tggcagtgga
     1081 cgaagtgctt ttgacaaaat cgtgatttct gaagctttga tagaaacttc gcctgtgtct
     1141 gttttcatga tttgtccagt tacaccaaag aaatctccaa ggtctgcatg tttgaatact
     1201 tcataggcct catcacctac ttggtctttt cttacataaa tttgaatttg gccttcacga
     1261 tcttgtagat gcgcaaatcc agttttccct tttcctcggc gagtcatcat acgaccagct
     1321 acactagcgg tcaatcccat ttctgctagt tcttcttttg ttcttggatc aaatagttca
     1381 tgcaattctt ctgagttgtg cgtgcgttca aatcgttttc caaatggatc gattccatct
     1441 tcacgcaatt gttccatttt ttgacgacgt acaagcattt gatcatttaa atcttctact
     1501 tgatgttgtt gttcctgtgt cacaaaagtt cctccattct catgttaatc tctttcctat
     1561 aatgccaatg aaacacaaaa aaagcaagcc gaacttggaa tttcggcttg aaaattcacg
     1621 aaaaacttag aaatcacaac gtttgtttca atagtacttt ttccgctttt tctacaaatt
     1681 gatccagcag gtcattgatt tcttgctgtt tttctgtctg attgattgct actttcactt
     1741 tcgctgctcg aggaatccct tttaaataat aagccgcatg tgttctgaat tctctacaag
     1801 ctactttttc tcctttgaga tctgctaagc gttgtaaatg aagtttagct gtagcaattt
     1861 tttcggctgg tgttggttca ggaatcagtt ctcctgtttc gagataatgc tgtgtgcggt
     1921 gaatcatcca tggattgccc agcgctgcac gaccaatcat cacaccgtct gcaccgacat
     1981 agtcaagcat ccattttgca tcttctggcg ttttgacatc cccgttcccc ataaatggga
     2041 tcgtcaaatg ttttttcact tctttcaata cttcccagtc cgcttttcct tcatacatct
     2101 gaacccgtgt acgtccatgc atagcgatgg cacttgctcc agctgattgt gctgctaatg
     2161 cattttctac agcaaagacg tgctcttcat cccaaccaat acgcatcttg attgttactg
     2221 ggatatttac tgctgaagaa acagcttgga ccatttcgta tactttctca ggatctaaaa
     2281 gccattttgc tcctgcttct gctttgatta ctttatttac tggacagcct atattgatat
     2341 ctattatatc ggcagctgtg ttttcttcga caaattgagc agcttcaact agactatctt
     2401 tatttccgcc aaatatctgt aaacttaatg gatgttctgt ttcttcaatg tggagcatct
     2461 ctaaagtttt tttgtttctg aaatgaatac cttggtcact gatcatttca catacgacta
     2521 agcccgcacc aaattctttg acagtgactc gaaatgctga atttgttatt ccagccatcg
     2581 gtgcaacaac gacacgatta gggatttcca cattgccaat tttccatgtg tgttccactt
     2641 aaatccctcc taaggcttct ttcttcaatc catctaattc ttcttcgcta tacttgtatt
     2701 tgttattaca gaaagaacaa accgcttctg caccgtgatc ttcgtctatc attgcttgga
     2761 tttcttctgc acctaacgcg ataattgctt ctgcaaattt ttcttttgaa cagtcacatt
     2821 tgaattgaac aggcattttt tccaaaatgt tcaattcttc tgtaccaagc aatcctttca
     2881 atatttcttc tggtgtctgt ccttcatcta agagggtaga gattcttggt gtcgttttta
     2941 attgttcttc gatcttgctg attgtttctt catcggcacc tggcatgatt tggatcatga
     3001 atcctcccgc cgtcttgatg ctgtcgtcag tattgaccaa tacactcagt ccaacagctg
     3061 aagggatttg ttctgagata gcaagatagt aagtaaaatc ttcagcgatt tctccagaaa
     3121 caatcggcgt ttgcccagaa aaaggttctt ttaatcctaa gtcttttgtg acagtaaaca
     3181 ttccttgtgt tccaactgct ccccgcacat cgattttacc ttcactattt agaggaagac
     3241 tgagatgcgg atttttgatg taacctttca catctccctt tccatttgca tctactacaa
     3301 ttcctgttgc tggaccatcg ccttggattc gaacagttag tttgtcttct cctttgagcg
     3361 tcgcaccaag tagcagacta ccgaccaatg tccgtcccaa agcagctgag gaggtgtgcc
     3421 aagtatcatg acgtctttgt gcctccgcta cggtatttgt tgcttgaacg gcgtatgctc
     3481 ggacgaatcc gtcgtgagca agtgccttaa ccaaataatc tttcatttgt tatgtactcc
     3541 tttttccgtt tttaaactca tagtgaatca tactagcaac tactgctttt ttcaactttg
     3601 gaatgcttct tttaaaaata attcagaatc atctagaaaa caggacatca ccctatttac
     3661 aacttaaata aagaaatctc ccgaaaaccg gtttttgttc cggttttcgg gagtcatatc
     3721 agatcaattg acctatattt ttttatttgt attgatcgtc tgaatgatta tcttctgatt
     3781 gttgagaatc tgaatcatgt tctgctttat tttcctcatt cagttcatgt tttgcttctt
     3841 caaaatcacg tttttcttcc gcttgttttt cagcgtcttt ttcttccaaa gcacgttttg
     3901 cttcttcaaa agttttcgct tctttttcgc ttggataatc atttgactca gtaccttcag
     3961 gcatcacacc gtgttcaaac aatgacttga ttgctttggc atccaatgtt tcgaattcca
     4021 aaagtttttc agcaatcagt ttatgctgtg cacgatgagc ttcgatgatt tctcgagctt
     4081 tatcatgagc ttccatcaag atttttcgta cttcttgatc aatctcgaaa gcgacttgtt
     4141 ccgagtaagc ttttgtttga ccatagtcgc ggccaacaaa cacttgatgg tttccttcat
     4201 attgaacagg acctaaacga tcactcattc catattctgt taccatgctt cgtgctagag
     4261 ctgtcgcttg ctcaaagtca ttggaagcac cagttgattg gacattgaag atgatttctt
     4321 cagctgtacg tccaccaagt aagcctacga tttgttcaaa catatcttct ttcgtcatca
     4381 agaactgatc ttcttttggt aaagcgatca tatatccgcc ggcgcgtccg cgagggatga
     4441 tcgttacttt gtggacgacc cgagcgcgac tcaatactaa tccgacaatc gtatgaccag
     4501 cttcgtgata agcaaccatt tcgcgttctt ttttgctgat tacgcgatct tttttagctg
     4561 gacctgcaat cacgcgatct tccgcttcat cgatatcaga agcatcgatt ttctttttat
     4621 ttcttctagc agctactaac gctgcttcat tcaagacatt ttctaaatct gctccggcaa
     4681 aacctggtgt ctgttgagcc acaactttca agtcaacgtc atctgccatc ggtttattcc
     4741 gtgcatggac tcgcaagatt gcttcacgac cttttacatc tggacgtcca actaaaatct
     4801 ggcggtcaaa acggcctgga cgtagtaacg ctgggtctag tacatcagaa cggttggttg
     4861 cagcaatgac gattactcct tcatttccgt cgaagccatc catttctaca agcaattggt
     4921 tcaatgtttg ttcgcgttca tcatgtccgc cgcccatacc ggcaccacgt tgacgaccta
     4981 ccgcatcaat ttcatcaatg aaaatgatag ctggtgcgtt tttcttcgct gtttcaaaca
     5041 aatcacgaac acgacttgct ccaacaccga caaacatttc tacgaaatca gaacccgaga
     5101 tcgagtaaaa cggtactcca gcttctccgg caacagcttt tgctaataag gttttacctg
     5161 ttcctggagg tccttctaaa agtacacctg ccgggatacg ggcacctaat tcaacaaatc
     5221 ggcgaggatc tttcagaaat tcaacaactt caactagttc ttgtttttct tcttccgcac
     5281 ccgctacatc agaaaaacgt acgcgattag cttttttgtc tgcttctttg gcttttgatt
     5341 taccaaagtt catgacacgg ccgccaccgc caccgccgcc gccttgttgt cccatcatca
     5401 tgtaaaagaa aaagatgatg attacgattg gcaagaagct aagcaatagg gaaacccata
     5461 taccgctgct tgattcttct tttactgttg tagcaacttt gttttctttt gccagttctg
     5521 ttacttggct caatgttgta tcatttggca aaatgattgt tgtgaaatgg gtagtagtcg
     5581 tatctgttga acctaagata gctaacccac cgctgttagc gacggtctgc tgttctttgt
     5641 attcaccagt gatcttatat acaccatttg caggctgcac ttcaaattct ttgatcttgc
     5701 ctgcttgtag ttgttctgtg aattttgagt actcgatatc tgacgatcgc tggccgttat
     5761 taccaaaaat gaaataaacg accattacca tcgctaagat tacgaggaca taatacaagg
     5821 catttttcat catgccgttg ttctttttgt tcatagttgc cctccctaag cgtcaattta
     5881 atttaacagt tttttatcaa tcctgaccat tctattgtac cataatttaa ttgagataca
     5941 gcattaattt gattgataaa cactaggttt caatacaccg atgtacggaa ggttacgata
     6001 tgcttctgca taatccaagc cataaccgac cacaaattca tttggtacgt tgaagccaac
     6061 ataatctgct tcgatattta ctactcttcc ttcaggtttg tcaagtagtg taacaatttt
     6121 gactgatttt gctttgcggt atttgaaaag atccactaaa taagcaagtg ttcttccgct
     6181 gtcaatgata tcttcaacga tcaacagatc gcgaccttct acattcgtat ctaagtcttt
     6241 gacgattttt acttcccctg acgagactgt tgcattcccg tagcttgaaa catccatgaa
     6301 atccaattcc aaatacgtat cgatagaacg aacgatatct gccatgaatg gtaccgctcc
     6361 ttttaagaca cctactacta ggggattggt gttttggtaa atttctgtta actctttgcc
     6421 tagttctttg caacgaactt gtatctcttc ttgtgagatc aaaattctct caatatcctt
     6481 ttccaacatc aggttctcct tccaattgct tctttttgat atttgtaaag aagtatgtag
     6541 tgtattttat cagtttcttc acgaatactc aaatatgaaa aggcaaaagg tatcaagcta
     6601 tatatatttc ttttggcatc tgtaatgatc caagcatgct gcctttgtga attaggtact
     6661 ttctgatcga taaaatatct agatactttt tttgtgagat tctctgttaa tttaatgcgg
     6721 tctcctgctt ttcttttttc gacatacagt tgcacatcag caggaatcca aatgtcatga
     6781 gaaaatagag accatgaatc tgttgctttg atgtcccctg cattttcagg tttgaccaga
     6841 ccaatccatt ctgtttcatt caaatatatt ccttgttctg gaatcagcaa caagcgctgg
     6901 tcttctgtct ggctagttgc ttgtttttct tttttagaga gtacaacaaa atgatactct
     6961 cttttcagta tccaattatt gccaatactg agttgccatt ggcttttttt ctgttgccac
     7021 tggtctaaaa tcgactccat ttgttgttct ttcaaaataa ttcctgtttt tgagaatgct
     7081 ttgtcgaaaa aggagtaaaa aaaatagtat cgttcagcat ctgtcatttc ttcaatcatt
     7141 tctgcagaaa atcgaaattg ttcctcccac tgttcgactt ccttttcgat caactgtccc
     7201 atatattttt gaatgacttg gtctgcccat tggatctgtt gcgaaaattg ctggaaatgt
     7261 ctcaacgcct gtgtattttc ttgtttcaat tgaggaacta ccaaatgacg aagccgattt
     7321 ctctggatat ctaacaattg gtttgtctga tcctcaaagt aaacaagctg cgattccgct
     7381 gcataagtat ataggtcttc tttgctaaaa ctcagcaacg gacgaatcag tctgcccgta
     7441 gcaaatggtt gtacttcttt gattccagaa aaagtattta actgtccccc acgaacgatt
     7501 ttcattaaca ccgtctcgat ttgatcatct ccgtgatgag cagtcatcaa cgtgtcatat
     7561 tgtccttctt ccatcacgcg agtgaattct tgataacgaa agttacgagc ttctgtttct
     7621 atggcggatt tggctggatc ttcccaaata cttacatata aagggagatt cttctcttca
     7681 caatatgagc gaagataggc agcttcttct gctgattctt ctcttaattg gtgattgatg
     7741 tggacgatac ctaaagaaaa accgatttgg cgttgcactt gtaaaagtaa atggagcaaa
     7801 accatcgaat caacaccacc tgataccgct gctaaaattc tcgatccttg ttgccagaat
     7861 ccttgttcat tcccgtgccg aataaatgac tgctgtaata gcggtgcttt tttcccgcct
     7921 ttttcaacgt tcatcctttc cacacccttt cttcctaaaa aaaagagagc gggaaaacgt
     7981 ctaaaacgac aattaacctg ccctctgttg atactatgac ccaaatagtc gcactatcat
     8041 tatttgcggc tttttagctg cgacgtccgc cacggccacc gcgctttcct tctgtgtttc
     8101 gcttcaatga agataagcgg tcatcgctat ctttcaaaaa tgaactcatc aatgaatcga
     8161 agtcttcttt ttgagctgaa ggttgactgc gagaaaaaga ttttttctgt gctggacgat
     8221 ttcggttgtc acggggatct ttttgttgat attttttagg acggctttct tctccagatt
     8281 cagcaggttg ttcctgcgct ttccggatag atagtccgat ttttccatca tcatttactg
     8341 aaataacttt aactgttact tcatctccta cacttaaaac atcatggatg tcttttacat
     8401 agccatttga cacttcgcta atatgaacta gacccgtttt tccagcacct agatcaataa
     8461 acgcaccaaa atttgtgatt cctgatactt ttccttgcag ttttgctcct acctcgattg
     8521 acataaaaaa aatgttcctc ctaattattt ccctttttta tattcctgct tattttgcag
     8581 ttgtagaact agttgattga tctgtttcat ttgtcgagct gtctgtattt gattctaata
     8641 taggataaac tttttcaccg tcttttgagt aaaagaattt gcttctggca agtttagcta
     8701 catactcatc atctttcagc aatgaaacat cttggtgcag ctgatcaacg tctgcgttga
     8761 cttctttcat ttcagtattt gcttcgactc tcaattcgtt caattgatgc atcctttgca
     8821 tgtcatggaa aatctgcaca cctattatcg cgaaaatgac caaagcacca acaaaaattg
     8881 cagccagtct gcgtcttcga aaaatcagtt gacgttgttg ttgctgaaat tcagcatact
     8941 ttttctttgc atattccgta tctagcgcag cgattttatt gctttttttc gtcacttgga
     9001 ttcgctccaa tctttttgat tgtctttttc attataccaa tatcgtgaaa gcttgtctaa
     9061 tctatttctc tagtgtttct gtgcgatttt cagaaataat ttcgtacatt ttttgtgcat
     9121 cttctttttt ggttgattca tgtaattccg taattttcac ttccaacgtt ttgttgccaa
     9181 actggacttt caaaatatcg cctactttga catcactgga agatttcgcc aatttaccgt
     9241 tgatttgaat tcttccttta tcagctactt cttttgctac tgatcgtctt ttgatgattc
     9301 gggaaatttt taaaaattta tctaatcgca tatcttattc ctcacttttt aattttttta
     9361 tttaatcgca ggattttctt accaaaaggc aacattagcc attctcgtac tgtaaacaga
     9421 cgaagacgaa tcactgcata aatgaacaca gaagccccga tcccaactcc tatcacgctg
     9481 acaaaaaaag cagtggaacg atgagttaca ggaccaaata ctatattcaa tacaccataa
     9541 tacagcagca gtactactgt catacaaaga aggctgaaca tcaacttccg tccaaaatgc
     9601 cgttctttcc aaaaagcatt tagctctttt gtttcaattc gaacaaaata ccaaagcgtc
     9661 acacctaatc ctaaaagggt agacaggctt gcacctgctg ttcctaataa accggtcaga
     9721 aaagaagtag tcaatccttt taccaatagt ccccatcctg cgccttttaa agaagggcga
     9781 aacgagttct tgctttgagc aatactttga taagcttgga tgactgcagt gaaagcaatg
     9841 gaaaaaacaa ataatacaag cgctgcattt cccgcataat ctttaaacaa cgcataattg
     9901 atataaggga gcagcaaggc taatcccaaa gacgcagcta atgctaaagc ggtcgtcaat
     9961 ctaagataga ttttggctgt ctgtaaaaac tgacgataga tccggttggt caaatgacga
    10021 gtcaacgcag gcaggaatgt cgcacttaat gcagtggcaa tcactaaacc taattgtact
    10081 aaaggctgac ctcgatcata gacccctttt gctatcttcg caccgtattc tgacagtccg
    10141 taaacttcca aagcattttt tacaaagaaa gagtcgatca gctggaaaaa gataagcaat
    10201 ccactataaa tcgaaaccag tccgccttct accagaaaac gccggatcaa gggctttgac
    10261 ggctgtttga acaatggaaa ccgtcggaag cttaacgctc ctccatgaat cttttgttca
    10321 tagtagtgaa ggatcccata tgcgcataaa ccgccaaaaa cagctccact cattgctgcc
    10381 gtccctgttt ggtacaccgt ccatccaagc tttctaaaag caaacgcact acagacaatc
    10441 acaaaaacac gaacgaactg ctcaactact tgagaaacag cagttggttc catcaagaat
    10501 cttccttgga aattccctcg ataaaaagat agaccaggca tcagcaaaaa ggtaaaggaa
    10561 actacttgga tcagcggttt caattgtatg tctcccatca tccaagcaat ccagctgcta
    10621 aatgcaaaga caaacaacca caaaaagaca cctgtccaaa atactaaggg ataaagattt
    10681 tgcaaagctt gctttcgctc gaaaggattc tttctttctg caacgtactt tgatatgaac
    10741 tgcggaagac cggataaagc taaagtcatt gctatcccat aaattggata aacttgctga
    10801 taaacataaa aaccttcgtc gccgactaag ttttggaggg gaattcggta aagggcactt
    10861 aatacttttg ctatgaaaga agcaatcgtc aatacaaatg ctccttgcat gacgcgccgc
    10921 atttctttat gatccaaatc attgctcctt cctacgctga cagatatttt tgctcacgca
    10981 acgctttgac aaacttttgg atttcctgaa gccatactgc ttcttccatt tggttgggga
    11041 tcgtcaagcg tatcaccatc tgttcttttt caacgcctag actggctttt agttttgtcg
    11101 ctgtcagtgc ttcgaataac tgttccacac tatagcgttt tgttccaact ttacttagag
    11161 taaagactat atgcgcctcc tgtttcctta tcgtttctag gagcgctcga tcgccatcca
    11221 ttttgatctg cccaattgtc aagagatgtg ctacttcttc tggatattct ccaaaacggt
    11281 cgagcagatc atcttctagt tcatctagca tttcttgcga atcaagttca cgaattcgtt
    11341 tatagatctc gattttctgc cgttgatctg ccacgtatgt ttcaggtaga tacgcatcga
    11401 caccgaggtc gatctcaaca gtggtcttct ccacttgact attctttcct tgtttacgag
    11461 aaacagcttc gcttagcatt tgagaataca tatcgaaacc gactgcatca ataaatccat
    11521 gctgctgagc acctaagaga ttacctgcac cgcgaatgga aagatcccgc atcgcaatct
    11581 tgaatccaga tcccagttct gtaaaatctt tgattgcctg aagacgtttt tcactgactt
    11641 cgttaaggat cttctgctgc tcatacataa agtaagcata tgcgacacgg ctgcttcttc
    11701 ctacccgccc tcgcaattgg tataacgtgg ataatcccat ataatcagcg ttctcaacga
    11761 acaacgtatt tgcgttcggg atatctactc ctgtttcaat gatggttgta gtgacaagaa
    11821 catcatattg accttcaata aattcaaaaa gcgtattttc taattgcgcc tctgtcattt
    11881 gcccatgagc ataaccgatc cgtgcttccg gaacgagttc ttggatttct tctacttttt
    11941 gttcgatcgt ttctacccga ttatataaat agaatacttg tccacctcgt cccatttctc
    12001 gatggattgc ttcgcgaata gccccaggat ttttttccat aacatacgtt tggatcggat
    12061 aacgattcgc aggcggtgtt tcgattactg aaagatcccg cacgcctaac atcgacatat
    12121 gcaaagttct cggaatcggt gttgcagtca gagtcaaaac atccacttgt gcacgtaact
    12181 gtttcaatcg ttccttatgt tttacaccaa aacgttgttc ttcgtcaatg atcagtagac
    12241 ccaaatcgct aaattcaata tcttttgaca aaattcggtg ggttcctaca acaatatcaa
    12301 cttgacctgt acgcagttgt tcgatggttt ctttttgttg ctttcttgtt ctgaaacgac
    12361 ttaatagccc gatatttacg gggaaacctt caaatctttc cagcatcgtt tcatagtgct
    12421 gctgtgccaa gatcgttgtt ggaactaaaa aggctacttg tttgctttcc tttatcgctt
    12481 taaaggccgc tcgaagcgct acttctgttt tcccataacc aacatcacct acgagtagtc
    12541 gatccattgg tttttctttt tccatatccc gtttgatctc cgctgcactt ctcaactggt
    12601 catcagtttc agagtagggg aaggcatttt cgaattcttt ttggtaggcg tcatcaggtc
    12661 caaaagcata tcctttttct gactctcttg cggcatataa tttgattaga tcgtcagcga
    12721 tatcctcgat ttttgaagag acttttcgct tggttttcgt ccattcactt ccgcctagct
    12781 tattgatgcg aggcgctttg gcttcagagg cgacatattt ttggatcaga ttcagttgag
    12841 taacaggaat aaacagtttg tcgtcgtttt gatacaaaat agtcatatag tcttggtgaa
    12901 cgccatctac ttctagcgtt tccatcccaa tgtatttccc gataccatga ttggcatgga
    12961 caacgtaatc tcctgctttt aactcattat agcttttcaa tcgctctgcg tttgttactg
    13021 tttgacgtct cgtttgtttt ttcgtcgttt tttggaaaat ttctttttct gtgatagcta
    13081 ctattttttc ttgaggaagt tcaaaaccag cctgcaaagc tccttcaact aattgtacct
    13141 gtcctctgat caatgcattt ttttctgttt ctactactaa tatgtcttca tctcgaagca
    13201 tttgttctgc tttgcgtatt ctgtcctttg tcggaataaa gatcacaaca gtctgttttt
    13261 gcttgcgcca acggtccatt tctgttttca gcaatggcat ttgtccgaag aactgttgca
    13321 tcgtccgata ttgaaaatta tggattgctt ggaaacgaag atttcccatc cctttttgga
    13381 agagtgagaa aaaggttgtg gtgaactttt ccttctgcac gattttttgt atatctgctg
    13441 aaaatgtctg ctcagaaaac acccgcaatt cgctgatttt ttgagtctgc cattctgccg
    13501 cttccctttc gatttcccga tttgtttcca tgattcgctg ataatcatca acgactagta
    13561 aactatttga agggaaataa tccaagatcg tcacagattc ttggtaaaga agatctgagt
    13621 agaattttgc ttcttctgtc gggacacctg cttgccagct tgaagtcagc tgaccgaagt
    13681 aatcttgcaa aaactcgcgg tctgcttctt ctgtggtcgc agcaagccgt ttttctagca
    13741 ttttttgcat ttgctctatt ccatgttcta gatcctctgg tccgtagact tgttcactcg
    13801 ttggggaaac ccagatttct tccgtttttt cgattgagcg ttgtgtttct gcttcaaaat
    13861 accgcataga atctatctct acatcaaata gttctacacg aacaggatag gttgtcgtta
    13921 acgggtaaat atcgataatg cttcctcgaa tactgaattc tcccggtttt gcgaccatct
    13981 cttctcgtac atagcccatc aaagtcaatt ggcgaggaag attttccacg tctatctcat
    14041 caccaatatg ccaattcaat tgcgcctgta gccaagtttt cttattcggc agtcgtttgt
    14101 gcaaagatgc gactggcaac aaatagatac ctgctttttt ttcttgaagt gcgttaagcg
    14161 ttgcaactct ctcgcctcgc gcttctggag aagaaaaggc catttcagca gaaagcactt
    14221 catccactgg aaataaatgg atctgatctt gcggaatcac atgttgcaga tcttctgcta
    14281 actgattcgc ataatacaaa tttggcatca gaataatcat gtttttattt gttttcttaa
    14341 acgctcgagc aaatagaagt gttttcgctg agcccgctaa tcctgtgatt agttgtctag
    14401 gcattttttc ttctaaattg ctgacccatt cttttacttg cggatctttt ccgactaatt
    14461 gaataatatc cattcctttt cctccttcct caattgaatc gattcatcgc atccacgaac
    14521 gaatgccctt ctatgagata ttcgactgct ttaacagatt gacacatgct ttcttctatc
    14581 aaaggctgat tttctttgct gaaaggacta agcacatgct ggactactgt ttttttccct
    14641 tctggcctgc cgattcccac cttgattcga tcaaagacat tcgtattcaa atgggaaata
    14701 atacttttga taccgttatg cccgccggca cttccctttt gacggagccg gatttttccc
    14761 actgccagat ccaaatcatc gtagattacg actaattcct ctggataaat cccaaaataa
    14821 gtcattagtg ggccgactgc acgaccagat tcattcatga atgtttgtgg tttgactaaa
    14881 aggatctttt ctccattatg aaaaaattcg gcaacttctg cttcgaacgg attttttttg
    14941 aaagtagctt gatgctcgcg tgcaatttta tccacgacca taaagccgat attgtgtttt
    15001 gtatattggt atttggtacc tgggtttcct aacccgacga tcattttcat ttttgttttc
    15061 cacctaattc taaaaagcag cacaaaaggc tggactgcta attagcggtc cagtccatcg
    15121 agcttttttc ttttttatac ttgtcaatgc atagtataac acaaaacaag gcgtttcggc
    15181 taaaagagaa tcgttaataa atgataaaga aattaaaaat ttcttcaaag tttaatgaaa
    15241 taattgatat ataaaaatag tgaaataacg cacatgtaag cttttacata taatacaacc
    15301 ttgttatctg ttatatttta tagccaaata gcgtgacatg gggcttgaga aatgttagca
    15361 taagcatggg aaaaaacaaa tcaatggaag gattgatact aaatgactgc agctgcagaa
    15421 aaaaaagatc atcaaaaagt tattcttgtt ggagacggcg ccgtaggttc tagctatgcc
    15481 tttgctctag ttactcaaaa tatcgctcaa gaagtaggta ttgtggatat caatactgct
    15541 aaaactgaag gagacgcaat tgacttgtct cacgctttgg catttacttc acctaaaaaa
    15601 atctattctg ctacttacgc agacgcacat gatgctgact tagttgttat tacagctggc
    15661 gcaccacaaa aaccaggtga aactcgttta gacttagtaa acaaaaacct aaaaatcaac
    15721 cgcgatgttg tcacacaaat cgttgaatca ggatttaacg gtatcttttt agttgccgct
    15781 aacccagtag atattttgac ttactcaact tggaaattct caggtttccc taaagaacgc
    15841 gtaatcggtt caggtacctc tcttgactca gctcgtttcc gtcaagcact tgcggaatta
    15901 gttgacgttg atgcacgtaa cgtccatgct tacatcttag gtgaacacgg agattcagaa
    15961 ttcccagttt ggtcacatgc aaatgttgcc ggcttgcaaa tctatgaatg ggtaaaaaac
    16021 aacccagaaa tcgatgaaga agctatggtt aacctattct ttggcgtacg tgacgctgct
    16081 tacacaatta tcgagaaaaa aggcgctact ttctatggta tcgcagtagc attagcacgt
    16141 atcactcgtg ccatcttaga tgatgaaaat gctgttcttc ctttatcagt attcatgaat
    16201 ggcgaatatg gattgaacga catctacatt ggtgcacctg ctgttatcaa ccgtcaaggt
    16261 atccaaaaag taatcgaaat tcctttaagt gacagcgagc aagaccgtat ggctgcttct
    16321 gctaagcaat taaaagaaat tctagatgaa gcttttgcta aactagacgc tgaataatct
    16381 ttgaacatga cggaaaacat ctattcggtg ttttccgttt ttttatttta tcacccttat
    16441 ggtttctaaa ttttttcata aagtagagat aaccttcgtc ttttcctcta aattatgcta
    16501 tcatgagacc gttccaccct aaacttttac ttaaggagtt gtcgatatga caagatcttc
    16561 gcatcgaaaa aaatcgaaac atgttgcttt gctttcagtt cttggtgttc tcttagtgct
    16621 gatcagcttt tatacctatc gcagtacata ctacacaaag cattttttgc cgaatacaga
    16681 aataaatgga ataaatgtca gcaatctaac agtcgaaaaa gcaaatgaaa aattaaaaga
    16741 agcttattct gataaaaaac tggctattaa agaagatgga aaagtctggc aggaagtacc
    16801 aaaatccgag ctcggctaca aagatgattt cacttcggag ctttctccga tcctaaatga
    16861 acaaaacgga tggacttggg gaatgactta tgtctcagcg gcagaaaaac aagaaataga
    16921 tcctgtcagt caaaaccacc aaaaactaga cactaccgtt cagacattga caactaagct
    16981 gactgatttg aacaaagacc ggacacctac tactgatgca acgatcgaaa aagcaggtga
    17041 ctctttcgtt atcaagcctg aagtcaatgg aaatacgatc gacgtcgatg ctgcggtcaa
    17101 acaattaaaa agtgcggtaa actctggtaa agatacaatt gaattgactg aattcaaaga
    17161 aaaaccaaaa gtaacatctg aagacagctc actgaaagaa caactcgcat ccatgaatgc
    17221 tatcgcaaat gtaaaagcta cttacagtat aaacggtgag actttccaga tcccatcaag
    17281 tgatattatg agttggctga cctacaatga tggaaaagtc gatttagata cagaacaagt
    17341 acgtcaatat gtaacagatt taggcacaaa gtataacaca agtacgaatg acacaaaatt
    17401 taaaagtaca aaaagaggag aagttacggt cccagttggc acttacagtt ggacgatcca
    17461 aacagacagt gagacagaag cattgaaaaa agcgattctg gcaggccaag attttactcg
    17521 ttcacccatt gtgcaaggtg gcacaaccgc tgatcatcca ttgatcgaag atacgtatat
    17581 cgaagttgat ctggagaacc agcatatgtg gtattacaaa gacggaaaag tggcccttga
    17641 aacagatatc gtttccggaa agccaactac acctacacct gctggagtct tctatgtttg
    17701 gaataaagaa gaagatgcaa ctttaaaagg aacaaatgac gatggaacac cttacgagag
    17761 tccggtaaac tactggatgc cgattgactg gacaggtgtt ggtatccacg attcagactg
    17821 gcaacctgaa tacggtggag acttatggaa aacccgcggt tcacacggct gcatcaacac
    17881 cccaccaagt gtaatgaagg aattattcgg gatggtcgaa aaaggaacgc ctgtattggt
    17941 cttctaacaa aaataatata ta
//
LOCUS       DACGLE010000055        17694 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0534
            SAMEA3304449-rid9560453.denovo.059, whole genome shotgun sequence.
ACCESSION   DACGLE010000055 DACGLE010000000
VERSION     DACGLE010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304449
            Sequence Read Archive: ERR985064
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17694)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17694)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702225.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702225.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 20:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,045
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 27 of 67
            Pseudo Genes (incomplete)         :: 25 of 67
            Pseudo Genes (internal stop)      :: 19 of 67
            Pseudo Genes (multiple problems)  :: 4 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17694
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304449-rid9560453.denovo.059"
                     /strain="VREN0534"
                     /db_xref="taxon:1352"
     gene            282..1133
                     /locus_tag="IXT20_001959"
     CDS             282..1133
                     /locus_tag="IXT20_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4860397.1"
                     /translation="MVKASQQNAKNSAFGYCCVAQRPKGAKTMKNGTIKRMADLKSAL
                     REENQKKQLSIFDETLGYAAIDFRIDHQQKGERSISWVTLIAAPSVKMKTVGEFLERL
                     EVYPDQTVVATVGLEGGYSPVHLAMDGLGKETWLVLCDEIAYHNFFSPPEHSELEVEF
                     DLKGAVKEHLKIQLETSKNPTETRLAGWLEQQEDSELLIGVLKEEKSLSELVQFCIQK
                     ASASKGKQSAMMVSDEVVFQWVREYYTTDIEMKNSSSVKGTMQTTPTPQVIETERKEE
                     EQQISLF"
     gene            1139..2464
                     /locus_tag="IXT20_001960"
     CDS             1139..2464
                     /locus_tag="IXT20_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002389883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4860398.1"
                     /translation="MKIAYDYPFKPLTPPKGFFKWCSSQITGYRWANKERTIVSTTRP
                     EAPIISKRLTKASNLNRSERCYPFALVLSSKTRVEIQSFGIWVGIENGVETYEIARTN
                     LECFCNDVHYQQHYFRNKWYGGLLNHGYMTGSYTNTQFYPNKWREKLAKNSPLKYCNL
                     KDISWYHLAHAYKYRREIEFLKLIHADKFADDMLFSAMHVIRDRVRGKSIDYRTVNMK
                     WLRKHKGILKNSTITFCEMRLKEAIRKCHGKPIAGIEEILTVEDLKRIPTCVGLTSFQ
                     KWAVAQDLDFGMYVDYLNMLDELGIGVDKENAMPKEFRAAHDEKAKLVGVKENKEYEK
                     EFNERVLQLKGLEKRLGDVQIVAPKEMGEMLVEGQNLRHCVGGQYYIRRHAKGETTIL
                     FVRKADKLDEPYLTLEMRNHRIVQLRGKRNQAAPPEIEQIIEKWVKTAN"
     gene            3116..3328
                     /locus_tag="IXT20_001961"
     CDS             3116..3328
                     /locus_tag="IXT20_001961"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4860399.1"
                     /translation="MDALRKLEVTLYNKQWFTLIGIEACLKNQDGFSAEEVLDQIQGC
                     LEEYKHVEGAMMEEYQQQNFAKLETV"
     gene            3426..3653
                     /locus_tag="IXT20_001962"
     CDS             3426..3653
                     /locus_tag="IXT20_001962"
                     /inference="COORDINATES: protein motif:HMM:NF037117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4860400.1"
                     /translation="MRKTISLTSNNIKVSGHIGTWYVYASRVYHGRRLFLVEHETYGD
                     HAANLILDKTGNCVMEDVWNGWEDYEVYIES"
     gene            3807..4259
                     /locus_tag="IXT20_001963"
     CDS             3807..4259
                     /locus_tag="IXT20_001963"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4860401.1"
                     /translation="MICRYCNKKVLKLEGGQYHCANCELDFFENQLIEFRSGLYREPT
                     VETDPFFFQYTTTKELLQMTYVELLSRLKLARGVYRELRKKVYGHNSQADDLDRENLK
                     AVTSAKLVIENIMIEKHGYYPASVFESVIDKEVLKNKKQMREVEKKHG"
     gene            4252..4503
                     /locus_tag="IXT20_001964"
     CDS             4252..4503
                     /locus_tag="IXT20_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002389958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4860402.1"
                     /translation="MVKFEDQPKQGYTGIRTLETIASVFEDIQASGDMGQGEKNLRYL
                     QLMNELEEDHGAMIVNPTKTDLNLPAVALYRKVSQARVF"
     gene            4528..4836
                     /locus_tag="IXT20_001965"
     CDS             4528..4836
                     /locus_tag="IXT20_001965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4860403.1"
                     /translation="MDKSKEIIFMPDYLVKDHTVLVLDSANNLHGFLSIESTDDHFVF
                     TNDIDLLKTVPKIIAEELATDIRASEGDYIPVTNKDTGEVSELGSDDLQIVFSAVEWC
                     "
     gene            4910..5374
                     /locus_tag="IXT20_001966"
     CDS             4910..5374
                     /locus_tag="IXT20_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002389888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4860404.1"
                     /translation="MNEPDMTNQFRVGEVILQRMIDRKEFEDIYRSVTRKNTVDAYYF
                     DAGRRIVTVFAYYLGSISGSSVDEERIVGELMALMATMERDSIPGVVVWDKISKLPGT
                     PEHSMQLASEKAIEDTFTFLLAAAKAEEPYYLNGGTETIVSMIAAEVEQDCR"
     gene            5393..5821
                     /locus_tag="IXT20_001967"
     CDS             5393..5821
                     /locus_tag="IXT20_001967"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4860405.1"
                     /translation="MLKCYGEAELLEMLKRNEFEEDSPIRSVEDLLGHLDQASPMMWV
                     TVYVPEVQSVYGIHRETLISTGQSHRSTSKPVALLEATGYQTSEHVFVSELAALLQME
                     VVPKNREIFELQLFERKRGKYMLRRWQMIGDQPVLIAQRG"
     gene            complement(5991..6857)
                     /locus_tag="IXT20_001968"
     CDS             complement(5991..6857)
                     /locus_tag="IXT20_001968"
                     /inference="COORDINATES: protein motif:HMM:NF012376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HAQ4860406.1"
                     /translation="MITLKNHKLELLQFTDIHIGQAPFNEEDQKTFAMIDQTLAKTTA
                     DLIVITGDLIWSDGVIEPTKGLEALAEIFNKYPIPLAITYGNHDSEETIDRHDLHELE
                     KKLFNHLALKANQFFDPNQKECFTIEIKDDNQLVNVLYFIDSGANALIDYESYDWVSL
                     EQIKWYDETFAKYQKINHTKDLLFLHIPLPEYLQAGERIVEGRFWEMNPRISAPKLNT
                     GLFSHLLENNHLLGTFCGHDHDNNFEGIYLGQRLIYGNVTGYNCYGDLPRGYRKITLK
                     PDQMTTSIETFE"
     gene            7038..7985
                     /locus_tag="IXT20_001969"
     CDS             7038..7985
                     /locus_tag="IXT20_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007474874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HAQ4860407.1"
                     /translation="MKQDSYSVKDANVILSKLILGSSDYLKLDNKDKVDRMFTRYVEE
                     GGRTFDTARHYRESEAVIGSWLKDKKREDYTIITKGCHPVREAPDTPRVNPESILADL
                     TTSLEMLATDYVDVLLLHRDDPSQPVGPLMETLSQLVDEKKIRSFGVSNWELPRIIEA
                     KKYCEDNGLNPLSFNSPNFSLAKVNEPRWENCVTADDLMIAWHEETDFPLISWSAQAE
                     AFFGNRFSPDDVNDPEMAEFVAVYFNDLNWKRLVICEELAKEKGVKPIQISLAYVLSQ
                     TFPSYATIGPEEEWQLLDSVEAMNIQLSAEEIMRLKKGE"
     gene            8018..8755
                     /locus_tag="IXT20_001970"
     CDS             8018..8755
                     /locus_tag="IXT20_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HAQ4860408.1"
                     /translation="MYSVRKELEKDPENTFKSLKEIGFEAVQLDGMRGHKETEIKALV
                     DKYGFKIAGMHIKHDRFFNDRDGIISESLLFGCKTIYDKYIDDEEQHTKGYIQTKQQL
                     VTTQATLGKLGFRIGLHCPEYDFNQTVEGRTILDYITDPVFGTAVYAEPDTYWISVAG
                     KDPLEEFRKFSGRAPIVHLKDYIKGFAPEDMDNNLTELGNGDVDFKAIVEWGEKSGVE
                     YYCIEQDYSKIGIFNSLAKSFDHLMSI"
     gene            8772..10025
                     /locus_tag="IXT20_001971"
     CDS             8772..10025
                     /locus_tag="IXT20_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002389894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HAQ4860409.1"
                     /translation="MKKNSRIVMMGVALVSGLVLAGCGNGSASNSDKVELSINYYNGA
                     ISKKTMDNAKKEFSDYKLSFKQIPANDDFDTKLKASLNSKSAPDITAINSNISDYLPY
                     ADKFVNLLDYDSETLAADFVEWKWDSCFTNDKKTQIAMPIDIGPTALFYNVDAFAKAG
                     LPTNDQEISKALKDEESLMAAAKQMKEKADMPFFQSSLSLLQSKNRTMTEHIYDENGK
                     LTYANGQLKEIWDYAVKAEKSGYTLGTKGNSADGANSVQKGMFGGMIEASWGINDLAE
                     NGVSPKQWMIAKSPFTASNYGGSYLAVIKTSSHPKEAAEVVKYLTNEDSQRANYEELA
                     LFPSNTKVYDDSFKAAKNELFGDEEFNHYFIDAANELEYVPSDPRESAAFKCFEDQII
                     LVAEQGKDPEKAWNDAVKKVEEMSK"
     gene            10126..11031
                     /locus_tag="IXT20_001972"
     CDS             10126..11031
                     /locus_tag="IXT20_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013921364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ4860410.1"
                     /translation="MNSESKSKKKFSFDKVAPYVFVSPFYILFLIFGLFPVIFSGVLA
                     FSKWDGLGDIQFVGLDNFTRLLRDADFYLSVKNTLIIWAVGTFPMLFLALILAFLVNL
                     KFIKHKELLKSIFFLPNITSVVAVTILFGLIFSNLDGLANALLQFLGFEPIKWVTSPF
                     WVRIVIALINIWMYLGYNMIIYLTGITKIPNDYYEAAVIDGATNMQIFWNITIPQLKP
                     IILFTVMMSTIGGLQVFSEAQVLVPQGATPEGGAMTIVYYLYNTAFTNNEYGYGSAVA
                     WALVLIILVFSLINWYLTTYRIKGE"
     gene            11034..11858
                     /locus_tag="IXT20_001973"
     CDS             11034..11858
                     /locus_tag="IXT20_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002389896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAQ4860411.1"
                     /translation="MSNRIFKILVRAFLAIGVFLSIFPFYWMIVMASKDNSEIYHIPP
                     DLMLGSELMNNIKTVFTETTFFSSLLNTLFVALTSTLLILFFDSLAGFSFAKLNFKGK
                     AFLFTFLLGTMMIPGQLNIIPSFYLMDKFGWVGSYKALIIPGMANAFGIFWIKQYCTD
                     AIPDSLLESAKLDGCTLFKAYYKVVLPIMKPALAFLALYSFMGAWNDYMWPLIILNDE
                     SKYTLMLALTQLQGLYKTNYPLVITGTLLATLPLLLVFTIFSKQLISGITEGAVKQ"
     gene            complement(11971..12258)
                     /locus_tag="IXT20_001974"
     CDS             complement(11971..12258)
                     /locus_tag="IXT20_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002389898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HAQ4860412.1"
                     /translation="MKEKNPYYRLKYVRIYKRRGKKRAIITIARMMLTAIYHMLSTGE
                     VWNPTDLYKIDMPDNLVQKQKDKAIKQATKLLISEGLLPDDFVRKDLEPAI"
     gene            12594..13184
                     /locus_tag="IXT20_001975"
     CDS             12594..13184
                     /locus_tag="IXT20_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4860413.1"
                     /translation="MEVNMKEKFIALQAKLMDLILLSGLILIGAMTIIGFFYGIVAAF
                     VYLETKSEDKWQVLKKRQTILISLLLEMSAVFVAGIFFLNARLLSQLQGGATIIISFF
                     LAVCTLILIPLYFILLHKLAVGTIFTKETFLNSVKVILIGFPTWFTQIILMIALSVLT
                     YLFPALSIITVGYLITRTNKIINVQKMKLASLQEVY"
     gene            13184..15193
                     /locus_tag="IXT20_001976"
     CDS             13184..15193
                     /locus_tag="IXT20_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002389900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIA"
                     /protein_id="HAQ4860414.1"
                     /translation="MKLSQREIDLLKLALDGEEETTVPQLKKQLKLSVHQIRYAIDKV
                     NRFLIEKKLNQIFIEKDRLVIEQRRKIKKEIDFFISHSTPEQFKFTAEQIEYFISLKL
                     LLSDQRLPINYFVETLSTSRTSVVNVLDRITQELAEEEIELSNVPRKGYMIEEYSFKR
                     FSYFITILKKMINIREIYSFYYQDNLYSKAGDLVFFDLFDLDILFDSLGETIQYSKQM
                     ASEIDDTNFLFLTILNYKYRELINAFEMTDRTEKLQMISSFISEIKQNNPNQMENNNL
                     AKRLIRYINRELADVFEVKDFKVASNALLYHIQRFIFRQENGIPFRDIDSEVLVKDHK
                     NLYYVIFAAIRSFQNGLFESVEAPEAVLITLYYINEIEKNAPKVFKAPRILIICGEGR
                     AISSVLKIKISNLIDSKWIDTISVFEFETPLIDHYDLIITTVRLPEVESDKVLYSDNV
                     FSESFFEQIKLKLSVGGYSFATQRLGKLSQIMNAIVEEVNDDINMNRLEAKIIKILAN
                     NLLQNNRPNEQKFIFDASMLKYHQAAQTWQNAIRISGQELLAKHSIEEQYVNNIISNI
                     ENFGPYMIIAPGVLLAHAGEQDGVLNNGFSMHVFHEPITFPGENVFPVSVIMTIAVKD
                     RTSYHVVERIVQWVLDEETIFSLADEKNRQRIEQMIRNILMMQEV"
     gene            15201..15506
                     /locus_tag="IXT20_001977"
     CDS             15201..15506
                     /locus_tag="IXT20_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012861070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HAQ4860415.1"
                     /translation="MTTDEISFALISKAGTAFSMMIEALGDAREKDFEKAEEKKQEAE
                     ALMNEAHNLQTNLIVNEVNGDDNQINALLIHAQDTLMNTILMSTIVDEFLNLYKAGE"
     gene            15508..15843
                     /locus_tag="IXT20_001978"
     CDS             15508..15843
                     /locus_tag="IXT20_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012860666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ4860416.1"
                     /translation="MEKLSICLACNLGSSTSILVNKMKQVAAGSQKLSSFDLMIDAIP
                     AGNISTEQVKDYQIIMLGPQIGHRRNEIEQKVASINIPVTVIDAADYGSMNAANILKE
                     AIYQIKTAN"
     gene            15856..17091
                     /locus_tag="IXT20_001979"
     CDS             15856..17091
                     /locus_tag="IXT20_001979"
                     /inference="COORDINATES: protein motif:HMM:NF014437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ4860417.1"
                     /translation="MTLDYINQVVGKIQTKMIPTLGKIEGNQVFKSMKKTFLYLNYVL
                     LISSVLAILKLVNEKFIHQQQIADLTSKLLLLLMDNFGWFFLGILCFLMFQEKGKFHY
                     YLCSAALYLMFSAKDLNLLSASKIETLFLALLALLVSFVLVSGYQLVLRGIKKIIKTP
                     MEFLDNLLLMIYFSVIFMVIYQILSQLKGIHLENILSWLNIDNPMMVFVIVFLEMFLW
                     YMGINGYGILAPIVLLFAISNLNANFQAIATGEAPQFIFTPNFWDYFLSVTGSGITGA
                     IVILSLLSKKTTLKNLGRTAASGTLFSVSEPIVFGIPVVMNPYFFIPFVIGTPILGVI
                     QWYVFKLGWVSLPTYFVADLPLPFSSFLATMDWRSLILTAATIALATLMYWPFYKAFE
                     KSYVEKNNDDKYQDLDLDF"
     gene            17107..>17694
                     /locus_tag="IXT20_001980"
     CDS             17107..>17694
                     /locus_tag="IXT20_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002389905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAQ4860418.1"
                     /translation="MLKSFFKIFCGNFILGLAYAKLMVPNEIINGGVTSLSLIFSNLT
                     SLNITLFTNVITVTLLAVSLLFLGKFFFFSSVVSSVSYLLFFNLFYRLPFSLHTNVVV
                     DFLLAVLLIAIGYYCCLSENSSTAGLDVFAVILNKKIPKISVAVFLRYINIAVLVLGL
                     FSYGIKAVILGVLFSFSFTKVMDLLMEKGADPRKFF"
BASE COUNT         5476 a         3028 c         3857 g         5333 t
ORIGIN      
        1 atttttttgt tcaaaaattc gaaacgaagt agataaaaag ctgttccaag atcgagttac
       61 gagtttggaa cagcttttgt ctacagtctg aggaacccgt gccgggttcc tctatttgaa
      121 ttttttggtt ttgcgctaca tttttaccat tgcagacgcc gactagctac gaaatagaat
      181 gagcctccag gctgcgccta ctcattctat ttctcgcaag tggcttcgcc aggcggcaag
      241 ggcaagcaat gtgctacgca cccttgccgc ctggctgctg catggtaaaa gcttcgcaac
      301 aaaacgcaaa aaattcagcg tttggctact gctgtgtagc gcaaagacca aaaggagcga
      361 aaaccatgaa aaacggtact atcaaacgaa tggcagactt gaagtctgca ctgagggaag
      421 aaaaccagaa aaaacagctc tcgatttttg atgaaacgct cggatatgcg gcaattgatt
      481 ttcgtatcga ccaccaacaa aaaggtgaac gaagtatttc gtgggtcacg ttgattgcgg
      541 caccttcggt caaaatgaag actgtgggag agtttttgga acgattagag gtctatcctg
      601 accaaacagt agttgcgaca gtcggactgg aaggtggcta ctcacccgtc catttagcaa
      661 tggacggtct agggaaagaa acatggctcg ttctctgtga tgaaattgcc taccacaatt
      721 ttttctcacc acctgaacac tcggaactgg aagttgaatt cgatttgaaa ggggcagtga
      781 aagagcacct aaaaatacaa ctagaaacgt ctaaaaaccc aactgaaaca aggcttgcgg
      841 gttggttaga gcaacaagaa gattctgagt tattaattgg tgtcttaaaa gaagaaaagt
      901 ccctatccga attggttcaa ttttgtattc agaaagcttc agcaagcaaa ggaaagcagt
      961 ccgctatgat ggtaagtgat gaggttgttt ttcagtgggt aagggaatat tacacgactg
     1021 atattgaaat gaaaaattca agttcagtga agggaaccat gcagaccaca ccaacacctc
     1081 aagtgattga aacagaacga aaggaggaag aacagcagat tagtctgttc taactactat
     1141 gaaaatcgca tacgattatc catttaaacc actcacgcca ccgaaagggt ttttcaagtg
     1201 gtgtagttcg caaattacgg gctatcgctg ggcgaacaaa gaacggacaa ttgtttcgac
     1261 aacacgacca gaagccccga ttatttccaa gcgattgaca aaagcttcaa atttgaatag
     1321 atcagaacgc tgttacccct ttgcgctcgt gttaagtagc aaaactcgtg ttgaaattca
     1381 atctttcggc atttgggtag gaattgagaa tggtgtcgaa acctatgaaa ttgctcgaac
     1441 gaaccttgaa tgtttctgca atgatgtaca ttatcagcaa cattatttta gaaataagtg
     1501 gtatggaggg ttgctgaatc atggttacat gactggctca tacacgaata cgcagttcta
     1561 tcccaacaag tggcgagaaa aactggcaaa aaattcaccg ctaaaatatt gtaatctcaa
     1621 agatatttct tggtaccatc tcgcccacgc ctataaatat cggcgggaga ttgagttctt
     1681 aaaactcatt catgcggata aatttgcgga cgacatgtta ttttcggcta tgcacgttat
     1741 acgggatagg gtacgaggga aaagtatcga ctatcgaacg gttaacatga aatggcttcg
     1801 gaaacacaaa ggaattttga aaaactctac aattacgttc tgtgaaatgc gattaaagga
     1861 agctatcaga aagtgtcatg gaaaaccaat agctggaatc gaagaaattc tgacggtgga
     1921 agacttgaaa cgtataccaa catgcgttgg gctaaccagc tttcaaaaat gggccgttgc
     1981 acaagacctt gactttggca tgtatgtgga ctacctcaac atgttagacg aactcggaat
     2041 cggtgtagat aaggaaaacg ccatgccaaa ggaattcaga gcagctcatg atgaaaaagc
     2101 aaaactggta ggcgttaaag aaaataaaga atatgaaaaa gaatttaatg agcgagtatt
     2161 gcaattgaaa gggcttgaaa aacgtctagg tgatgttcaa attgttgcac caaaagaaat
     2221 gggcgagatg ttggtggaag ggcagaacct tcgccattgt gtcggtggtc agtattatat
     2281 tagaagacat gcgaagggag aaacaacgat tctctttgtt cggaaagcgg acaagttaga
     2341 tgaaccctat ctcaccttag aaatgaggaa tcaccgtatt gttcaattgc gtgggaagag
     2401 aaaccaagcg gcacctcctg aaatcgaaca gataatagaa aaatgggtta aaaccgcaaa
     2461 ctgataagac gtttaccaga gtgatgaaaa gcacagcatt ctggtaagcc gcttatcggg
     2521 taaaactggc gcttcgcctg cggtgcagtt gcccttggct caaaacgcga cagtcttctt
     2581 ttgcctagtg acagaatcta accgcttgat ggctacgtgc cttcgccagt cgcagttgct
     2641 ttggttggct gcgttcctcc gccaagtcag ccgattcagt catttttgcc agggcaaaaa
     2701 agcaaaagga ttgaaaggtc ttgctcgaag catagctccg acttctcgca agacggcggg
     2761 gaaatcaccc gcctgaaaat tccgagcgaa ttttgttttg ttcgtcaatc gccgctccta
     2821 ttccggtttc attttgtgtg caaaggttgt ttgctgtttg tggttataga tcaaacccgg
     2881 tggcaggtaa agggtataca agcggcaaaa tccgccgccc tttacctgcc accgggcccg
     2941 atctagagca taaccacagc aaacgaccta agcacactgg aaaaaatgaa cccatgacaa
     3001 ataggaacaa agggcacgac tggacaaacc aaaattggaa tttctttaaa gattccagct
     3061 ccgccctagc tcctaaaaaa atgggtttca aaccaacaaa aaaggagttt tttacatgga
     3121 tgctttaaga aaattggaag tcacacttta caacaaacaa tggtttacac tgattgggat
     3181 tgaggcttgt ttgaaaaatc aggatggttt ttccgctgaa gaggttttgg accaaataca
     3241 gggttgtctg gaagagtaca aacatgttga gggggcaatg atggaagaat atcagcaaca
     3301 aaattttgct aagttggaaa ccgtgtgata ccagctgaac aatcggaaat gcaaggcatg
     3361 taattcggat gacgacctgg tccttgccag gttgttttcc ggaatcattg aaagggtgtt
     3421 gaattatgcg aaaaactatt tcattaacat cgaacaatat caaggtaagc gggcatatag
     3481 gtacgtggta tgtatacgcc agtcgagtgt atcacggacg tcgtttattt cttgtagaac
     3541 atgaaaccta cggggatcac gctgccaact tgatactgga taaaacggga aactgtgtca
     3601 tggaagatgt ttggaatggg tgggaagatt atgaggtgta tattgaaagc tgaagggaaa
     3661 gagtaggcac cagtctgatc ttaggatgac ctaagctgag agtcttcagc ttgggtatgt
     3721 tcttgtaggc taattcttgg taaaaaagaa gagcagcatt gtttatttag catgatatac
     3781 tatttgtgaa aacgggggga tttgtgatga tttgtcgata ttgtaataaa aaggttttga
     3841 agctagaagg gggccagtat cactgtgcca attgtgagct ggattttttt gagaaccaac
     3901 tgattgagtt caggtctgga ttgtatcgag aaccgaccgt cgaaacagat ccgttctttt
     3961 tccaatacac gacgacaaaa gaattattgc agatgaccta tgtagaattg ctgtctcggc
     4021 tgaagctggc gagaggcgtc tatcgtgagt tgaggaaaaa agtttatggc cacaatagcc
     4081 aagcagatga tctggatcgc gagaacctga aggcagtcac ttcagcaaag cttgtcatcg
     4141 aaaatattat gattgaaaaa catggctatt atcccgccag cgtttttgag tctgttatag
     4201 ataaagaagt tttgaaaaat aaaaaacaaa tgcgagaggt ggaaaaaaag catggttaaa
     4261 tttgaggatc aaccgaagca ggggtataca ggtatccgga cgctagaaac gattgcgagt
     4321 gtatttgagg atattcaggc cagcggtgac atgggacagg gcgagaagaa tttgcgatac
     4381 ttgcagctga tgaatgagtt ggaagaggac cacggagcta tgattgtgaa tccgactaaa
     4441 acggatctga atttgcctgc ggtcgcatta taccggaagg tttctcaagc tcgagtattt
     4501 taggcggaac ttgagaggag tttacgaatg gataaatcga aagaaatcat ctttatgcca
     4561 gactatttag taaaagatca tacggtgcta gttttagatt cagcaaacaa ccttcacggt
     4621 tttttgagta tagaatcaac cgacgatcat tttgttttta caaatgatat agacctgctc
     4681 aaaaccgtcc cgaaaattat tgcggaagaa cttgcaactg atattcgcgc ttctgaaggc
     4741 gactatattc cagtaacaaa taaagatact ggcgaagtat ctgaattggg ttcagacgac
     4801 ctccaaatcg tattttctgc ggtagagtgg tgctaaaggt agcggctgag tctaacgatc
     4861 atgatgaatg catggattca actaatagga gaaaagaagg tagaaattaa tgaacgagcc
     4921 ggatatgacg aatcagtttc gagttgggga agtcattctt cagcgaatga ttgatcggaa
     4981 agaattcgaa gacatttatc gctcagtgac ccgaaagaac acggtggacg cctactattt
     5041 cgatgcaggg cgtagaatcg taacagtatt tgcttattac ttgggaagta tctctggttc
     5101 ttcagtcgat gaggaaagaa tcgttgggga gcttatggct ttgatggcaa caatggagcg
     5161 cgacagtatt cctggtgttg ttgtttggga taaaatttca aaacttccag gcacaccgga
     5221 acattccatg cagcttgctt cagaaaaagc aatcgaagac acctttactt ttttacttgc
     5281 agctgcaaag gctgaagagc cgtattatct caatggtggg acggaaacta tcgtatcaat
     5341 gattgctgct gaagtagaac aggattgtag gtagcagcag gaggcagaaa atatgttgaa
     5401 atgctatggt gaagctgagc tactagaaat gcttaagaga aacgagtttg aagaggattc
     5461 accgattcgg agtgttgaag atctgcttgg acacctggat caggccagtc caatgatgtg
     5521 ggtgacggtg tatgtaccag aagttcaaag tgtgtacggt attcatcgag agacgttgat
     5581 ttctacggga caaagccata ggagtacatc taagccagtt gctttgttag aggcgacagg
     5641 ttatcaaaca agtgaacatg tctttgtttc tgaattagcg gcgcttttac agatggaggt
     5701 agtccccaag aatcgagaaa tctttgaatt acagctgttt gagcgaaaac gggggaagta
     5761 catgttgcgt cgctggcaaa tgattgggga tcagccagta ctaattgccc aacgtggcta
     5821 attattattt aaagatgagt gttgtgtaag ctgaggtgac ctttattgga tgaaaagcaa
     5881 caaaaacgac aactactaat taaattagaa tcagtcctaa aatgaatgac tattttagga
     5941 ctgattttgc tacataaaaa acagctagca gattactagc tgttctttct ttattcaaaa
     6001 gtttcgatgg aagtcgtcat ttgatccggt tttaaggtga tcttacggta gccccgtggc
     6061 aaatcgccat aacagttata accggtgaca tttccgtaaa tcaatcgttg gcccagatag
     6121 atgccttcga aattattatc atgatcatga ccgcaaaaag tgccgagtaa atggttgttt
     6181 tctagtaaat ggctgaataa gccggtatta agctttggtg ccgaaattcg cggattcatt
     6241 tcccaaaaac gcccctctac gatccgttcg ccggcttgta gatattcagg taatggaatg
     6301 tggaggaaca atagatcttt tgtatgatta atcttttgat atttcgcgaa agtttcgtcg
     6361 taccacttga tttgttctaa agaaacccag tcgtagctct cataatcaat taaggcattg
     6421 gcgccggaat cgataaaata aagaacatta actaattgat tatcatcttt aatttcaatt
     6481 gtaaaacatt ctttttgatt tggatcaaag aattgattgg cttttaaagc cagatgattg
     6541 aagagttttt tctctaattc gtggagatcg tgacgatcaa tggtttcttc ggagtcgtga
     6601 ttgccgtaag tgattgccaa tggaatcggg tatttattga aaatttcggc taaagcttct
     6661 aacccttttg taggttcaat aacaccatct gaccaaatta agtcgccggt tatcacgatc
     6721 aaatcagcgg ttgtcttcgc taaggtttga tcgatcatgg caaaagtttt ttgatcttct
     6781 tcattaaaag gagcttgacc gatgtggata tcggtaaatt gcaatagttc aagtttgtga
     6841 ttttttaaag taatcaaggg taacatcctt tcttaatacg ttattaaaat aaaccctatc
     6901 aaaaaataat gcaaacgctt taataacttt ttttaagatt aaaaaaataa aatatgtcat
     6961 tcaagacgtt gaaagcgatt ttagaatttg ctattctttg ggtgtgttga agagagcaaa
     7021 aagaaagggg cttcagaatg aaacaagatt cttattcagt taaggatgca aacgttattt
     7081 tatcaaaatt gatcttgggt tccagtgatt atttgaagtt ggacaacaag gacaaggtcg
     7141 ataggatgtt cactagatac gtagaagaag gtggacgaac ttttgacact gccagacatt
     7201 accgtgaaag tgaggcggtg attggcagtt ggttaaagga taaaaaacgt gaggattaca
     7261 ctattatcac taaaggttgt catccagtgc gggaagcacc agatacgccg cgtgttaatc
     7321 cagaaagtat cttagcagac ttgactacta gtttagaaat gttggcaact gattatgtcg
     7381 atgtgctttt actgcaccga gatgatccta gccagccggt cggtccatta atggaaacct
     7441 taagccaatt ggttgatgaa aagaaaatcc gatcttttgg tgtttctaac tgggagttac
     7501 cacgaattat tgaggcaaaa aaatattgtg aagataacgg attaaatcct ttgtcattca
     7561 atagtccaaa ttttagttta gccaaagtta atgagcctcg ttgggaaaac tgtgtgacag
     7621 cagacgatct gatgattgct tggcatgaag aaactgattt tcctttgatt tcttggtcgg
     7681 cacaagcaga agcattcttt ggaaatcgct tctcaccgga tgatgttaac gatccggaga
     7741 tggcagagtt tgttgctgtt tattttaatg acttgaattg gaagcgctta gtgatttgtg
     7801 aagagttggc aaaagaaaaa ggtgtcaagc caatccaaat ttctctggcc tacgtgttaa
     7861 gtcaaacttt cccctcatat gccacaattg gtccggagga agaatggcag ttacttgatt
     7921 cagttgaagc gatgaatatt caattatctg ctgaagaaat catgcgtctg aagaaaggag
     7981 aataagccag ttggataaaa caaagattgc ggcccaaatg tattccgtcc gtaaagaact
     8041 ggaaaaagat ccagaaaata cttttaaatc attgaaggaa attggttttg aagccgttca
     8101 gcttgatggt atgcgggggc acaaagaaac tgaaatcaaa gcattagttg ataagtatgg
     8161 tttcaaaatt gccgggatgc acattaaaca tgatcgtttt ttcaatgatc gagacggaat
     8221 tatttctgaa tcattattgt ttggttgtaa aacaatctac gataaatata tcgatgatga
     8281 agagcaacat acgaaaggct atatccaaac gaaacaacaa ttggtcacga ctcaagcgac
     8341 tcttggaaaa ctcggtttta gaatcggcct tcactgtccg gaatatgatt ttaatcaaac
     8401 agtcgaaggg cggaccattt tggattatat tacagaccca gtttttggga cggcggtcta
     8461 tgcggagcca gatacttatt ggatctcggt agctggaaaa gatcccctag aagaatttag
     8521 aaaattctct ggtcgggcac cgattgttca tttgaaagat tacatcaaag gttttgcacc
     8581 ggaagatatg gataataact tgacggaatt aggtaacgga gacgtcgatt ttaaagccat
     8641 tgttgaatgg ggcgaaaaaa gtggtgttga gtactactgt atcgaacaag attattccaa
     8701 aatcggtatt tttaacagtt tagcgaaaag ttttgatcac ttaatgtcaa tttaatctta
     8761 ggagcgagaa gatgaagaaa aatagtcgta ttgtcatgat gggtgtcgca ttggtaagtg
     8821 gtttggtatt agcaggttgt ggaaatggca gtgcctcaaa tagtgataaa gtggaattat
     8881 ctatcaacta ttacaatggt gcgatcagta aaaaaaccat ggataatgct aaaaaagaat
     8941 tttctgacta caaattgagt tttaaacaaa ttcctgccaa tgatgatttt gatacaaagc
     9001 taaaagccag cttaaattca aaatcggcac cagatatcac agcaatcaac agtaatattt
     9061 ctgattacct gccatatgct gataaattcg taaacctatt ggattacgat agtgaaacat
     9121 tagccgctga ctttgttgaa tggaagtggg attcttgttt tacaaatgac aaaaaaacgc
     9181 agattgccat gccaattgat attggcccaa cagctttatt ctataatgta gacgctttcg
     9241 caaaagctgg tttgccaacg aacgatcaag aaatcagtaa agcattgaaa gatgaggaaa
     9301 gcttgatggc tgctgccaaa caaatgaaag aaaaagccga tatgcctttc ttccaatctt
     9361 ctctttcatt gttgcaatct aaaaatcgca cgatgactga acatatttac gatgaaaatg
     9421 gcaaattgac ctacgctaac ggtcaattaa aagaaatctg ggattacgct gttaaagcag
     9481 aaaaaagcgg ttacactttg ggaacaaaag gaaatagtgc tgatggcgcc aactccgttc
     9541 aaaaaggcat gtttggtggc atgatcgaag cttcttgggg gatcaatgac ttagctgaaa
     9601 atggcgtttc accaaaacaa tggatgatcg caaaatctcc atttaccgcg tctaactacg
     9661 gtggttcata tttggcggtg atcaaaactt caagccatcc aaaagaagct gcggaagttg
     9721 tgaaatattt gacgaatgaa gacagccaac gggctaacta cgaagaattg gctttattcc
     9781 catcaaatac taaagtttat gatgattcct tcaaagcagc gaaaaatgaa ttattcggtg
     9841 atgaagaatt taatcactac tttatcgatg ccgctaacga attggaatat gtaccatcag
     9901 atccacgtga aagtgctgct ttcaaatgtt tcgaagacca aatcattttg gtagctgaac
     9961 aaggcaaaga tcctgaaaaa gcttggaacg atgccgtgaa gaaagtagaa gagatgtcta
    10021 aataatcaga atgtgatttc gggatatgac atcgatcaaa ttttaaaaat tggtgggtgt
    10081 catagacccg taaatggagg tctaaggatg gagattggtg agatgatgaa ttcagaatcg
    10141 aaatcaaaaa agaaatttag ttttgataaa gttgcccctt acgtttttgt atccccgttt
    10201 tatattttgt ttttgatctt tggtctgttt ccagttattt tctcaggcgt cctggccttt
    10261 agtaaatggg atggcttagg ggatatccaa tttgtcggtt tggataattt tacccgttta
    10321 ttgcgggatg ctgactttta tttatcagtc aaaaatacgc taatcatctg ggcagttggg
    10381 acattcccga tgttgttttt ggcactaata ttagctttct tagtaaattt gaaatttatc
    10441 aaacataaag agttactaaa atcgatattt ttcttaccaa atatcacttc tgttgtagcg
    10501 gtgacaattt tatttggctt gattttctca aatctggatg gcttagccaa tgcgctatta
    10561 cagtttttag gatttgaacc aattaaatgg gttacgtcac cattttgggt tcgaatcgtg
    10621 attgctttga tcaatatttg gatgtactta ggctataaca tgatcattta tctaacaggg
    10681 attacaaaaa ttccaaatga ttactatgaa gctgccgtta ttgatggcgc aaccaatatg
    10741 cagatttttt ggaacattac gatacctcaa ttaaaaccga ttatcttgtt tactgtgatg
    10801 atgtcaacca ttggtggttt gcaagtcttc tctgaagcac aagtattagt accccagggg
    10861 gcgactcctg aaggtggggc gatgaccatc gtttactact tgtataatac ggcgtttaca
    10921 aacaatgagt acggatatgg atcagcagtt gcttgggcat tagtcttgat cattctggtc
    10981 ttttcgttga tcaactggta cttgacgact tatcgaatca agggggaata aacatgagca
    11041 atcggatttt taaaatactt gttagagcct tcttagcgat tggtgtcttt ctttcgatct
    11101 tcccatttta ttggatgatt gtaatggcca gtaaagataa tagtgaaatc tatcatattc
    11161 caccagattt gatgctaggg tctgaactga tgaataatat caaaacggtt tttactgaaa
    11221 caacattctt tagctcgttg cttaatactt tatttgtggc actaacttcg acactgctga
    11281 ttttgttctt tgattcctta gcgggttttt catttgccaa gctgaacttt aaagggaagg
    11341 cctttctctt tactttctta ttgggaacca tgatgattcc gggtcagctg aatattattc
    11401 cttcatttta tttgatggat aaatttggtt gggttggttc ttacaaggcg ttgattattc
    11461 cagggatggc aaatgccttt ggtattttct ggatcaagca gtattgtacc gatgccatcc
    11521 ccgacagttt gttggaatca gcaaaattag atggttgtac actttttaaa gcctattaca
    11581 aagttgtatt gccaattatg aaaccagctt tagcattttt agcactctac tcttttatgg
    11641 gtgcgtggaa tgactatatg tggccattga ttatcttaaa cgatgaatca aaatacacgt
    11701 tgatgttggc tttgacacaa ctacaaggac tttataaaac aaattatcca ttggtaatta
    11761 ctggtacgtt actggcaact ttgccactac tcttagtctt tactattttc agtaagcaat
    11821 tgatttctgg tatcacagaa ggtgccgtta agcaatagaa agaggtatta gtgtgaaaac
    11881 aaaaccactt agtatcggag atacacccaa gaatagagcg catggcaaat aaacgtcaaa
    11941 aaccgcaggt ctgacgatta tgaaagttat ttagatggct ggctctaagt cttttctaac
    12001 aaaatcatct ggcaataaac cttctgaaat cagtaatttg gtcgcttgtt taattgcttt
    12061 atctttctgt ttttgaacaa gattgtctgg catatcaatc ttatataagt cagtcggatt
    12121 ccaaacctca ccagttgaaa gcatgtggta gatagctgtg agcatcattc gagcaatcgt
    12181 gatgatagcc cgtttcttac cacgtcgttt gtaaatgcgc acgtacttca aacggtagta
    12241 cggattcttt tctttcacca aaacggctgc gtgtgtacat caataccaca acaaacggga
    12301 taaacgactt tcatgattct tcgccccttt ctaaaagtag cggagaggca gtgactggtc
    12361 tgggcagcat ttaacaggat ttgttaaaac aatgattagt gtgcggactt ctatagttcc
    12421 gctcatttgt gcttgaacgc cccgacctta cactggttaa tatgctggtt caaacaatcg
    12481 aggtttccta caactcacct caccgtgctt tgtagtatgc ctctcctaat tctattataa
    12541 acggaaatcc gcgtcttcat tactatttga ctcgaggagc ggagcgagtc gagatggagg
    12601 ttaatatgaa ggaaaaattt atcgccttac aagcaaaatt aatggacttg attttattga
    12661 gtggtttgat cttgatcggg gcaatgacga taattggttt cttttatgga atcgttgcgg
    12721 cgtttgttta tctagaaact aaaagtgaag ataaatggca agttttaaaa aaacgtcaga
    12781 caatcttgat tagtctctta ttagaaatgt cagcagtttt tgtagccggt atctttttcc
    12841 taaatgcgcg tttgctttcg cagttgcaag gcggtgcaac aattatcatt agtttctttt
    12901 tagccgtttg taccttgatc ttaattccac tgtatttcat tttattgcac aagctagcag
    12961 ttggaacaat ttttacgaag gaaacatttc ttaatagtgt caaggtcatc ttaatcggtt
    13021 ttcctacctg gtttacccaa attatcttaa tgatcgcgct gagtgttttg acttatctgt
    13081 ttccggcttt atcgatcatt acagttgggt acttgattac ccgcacaaat aagatcatta
    13141 atgtgcaaaa aatgaaatta gctagtttgc aggaggtcta ttaatgaaat tgagtcaacg
    13201 ggaaattgat cttttaaagc tggctttaga tggtgaagaa gaaacgactg tgccgcaact
    13261 aaaaaaacaa ttgaagctct cagtccatca aattcgctat gctattgata aagtgaaccg
    13321 ctttttaatt gaaaaaaagt tgaatcaaat ttttattgaa aaagatcggc tggtcattga
    13381 acaacgacga aaaatcaaaa aagaaattga tttttttata tctcactcga cgccagagca
    13441 gtttaaattt accgcagagc aaattgaata ttttatctca ttgaaactgc tgctaagtga
    13501 tcaacgtttg ccaatcaatt attttgtaga aacattatcg acttcccgga cttctgtggt
    13561 aaatgttttg gaccgaatca cacaggaact ggctgaagaa gaaatcgaat tatcgaatgt
    13621 accacgaaaa ggttacatga ttgaggagta tagttttaaa cgattttcct atttcatcac
    13681 gattttgaaa aaaatgatca atattcgtga aatctatagt ttctactatc aagataattt
    13741 gtattcaaaa gctggcgact tagtattttt tgacctcttt gatttggata tcctctttga
    13801 ttctttaggg gagacgattc agtattcaaa acagatggcc agtgaaattg atgatacgaa
    13861 ctttttattt ttaacgattt tgaactataa gtatcgtgag ttgatcaatg cctttgagat
    13921 gactgatcga acggaaaaat tacaaatgat ttcaagtttt attagtgaaa tcaaacaaaa
    13981 taatccgaat caaatggaaa acaacaatct agccaagcga ttgattcgct atatcaaccg
    14041 tgaattagcc gatgtttttg aagtaaagga ttttaaagtg gcctctaacg cactactata
    14101 tcatattcaa cgctttattt ttagacagga aaatggcatt ccttttcgcg atattgatag
    14161 cgaagttcta gtaaaagacc acaagaattt gtattacgtg atttttgcgg caatccgttc
    14221 ttttcaaaat gggttgtttg aaagtgtaga agcacctgag gcggtattga tcacattgta
    14281 ttacatcaat gaaattgaaa agaatgcacc aaaagttttt aaagcgccgc gaattctgat
    14341 tatttgtggt gaaggccgtg caatttccag cgtgttaaaa atcaaaatca gtaatttgat
    14401 cgattcgaag tggatcgata cgatttcagt ttttgaattt gagacaccct tgatcgatca
    14461 ttatgatttg atcattacta cggttcggtt gccggaagtc gagtcagaca aagttctata
    14521 ctcggacaat gtcttctccg aaagtttctt tgaacaaatt aaattgaaac tatcggtagg
    14581 tgggtactca tttgcgaccc aacgccttgg caagctttca caaatcatga atgcaatcgt
    14641 cgaagaggtt aacgatgata tcaatatgaa tcgtcttgaa gcaaagatta tcaaaatttt
    14701 ggcgaataat ttgctgcaaa ataatcgtcc caatgaacaa aaatttatct ttgacgccag
    14761 catgttgaaa tatcatcaag cagcgcaaac atggcaaaat gcgattcgca ttagcgggca
    14821 ggaacttttg gcaaagcact cgattgaaga acaatatgtt aataatatta tcagcaatat
    14881 tgaaaatttt ggaccttata tgatcattgc accgggtgtc ttgctggctc atgctggtga
    14941 acaagatggc gtgcttaata atggctttag tatgcatgtt tttcatgaac cgattacttt
    15001 tccaggtgaa aatgtttttc cggtaagtgt gatcatgaca attgccgtaa aagatcgcac
    15061 atcttatcac gttgtcgaaa ggatcgtgca atgggtctta gatgaagaaa cgattttttc
    15121 gttagctgat gagaaaaaca gacaacggat cgaacaaatg atccgaaata ttttgatgat
    15181 gcaagaggtg taacatgaca atgacaacgg atgaaatatc tttcgccttg atttctaagg
    15241 ctggtacagc tttttcaatg atgatcgagg cattgggaga tgcccgcgaa aaggattttg
    15301 aaaaagcaga agaaaagaaa caagaagccg aggctttgat gaatgaagcc cataatttac
    15361 aaacgaattt gatcgtaaat gaagtcaatg gcgatgataa tcaaatcaat gccttattga
    15421 ttcacgccca agacacatta atgaatacaa tcttgatgtc gacgattgtc gatgaatttc
    15481 ttaatttata taaggcagga gaataaaatg gagaaattat caatttgctt agcgtgtaat
    15541 ttagggtcat ccaccagtat cttggtgaat aaaatgaaac aagtggcagc cgggagtcaa
    15601 aaattgtctt cttttgattt aatgattgac gccattccag caggaaatat cagcacggaa
    15661 caagtgaagg attatcaaat tattatgttg ggaccacaaa tcggacacag gcgcaatgaa
    15721 attgagcaaa aagtagctag tatcaatatt ccggtaacag tcatcgatgc agctgactat
    15781 ggcagtatga atgccgctaa tattttgaaa gaagcaattt atcaaatcaa aactgctaat
    15841 taaggatgga tagccgtgac tttagattat atcaatcagg tagttggaaa aatccaaaca
    15901 aaaatgattc cgactttagg taaaatcgaa ggaaatcaag tttttaaaag tatgaaaaaa
    15961 acttttttgt accttaatta tgtcttattg atttcctcgg ttttggctat tttgaaactt
    16021 gtaaatgaaa agtttatcca ccaacaacag attgctgatt tgacgtctaa gttgttgtta
    16081 ttactgatgg ataattttgg ctggtttttt ctagggattc tttgcttttt gatgtttcag
    16141 gaaaaaggaa agtttcacta ctatctatgc agtgcggctt tgtatctcat gttttcagca
    16201 aaagatctga atttattatc agcctctaaa attgaaacac tatttttagc attgttggct
    16261 ctgttagtta gctttgttct ggtgagtggt tatcaattag tgctgcgggg aattaaaaag
    16321 atcatcaaga caccaatgga atttttagat aatttgttac tgatgattta tttcagcgtg
    16381 attttcatgg ttatctatca aatattgtct cagcttaaag gaattcattt agaaaatatt
    16441 ttgagttggt taaacattga taacccaatg atggtttttg tcattgtttt tcttgaaatg
    16501 tttctttggt acatgggcat caatggctat ggcatattag cgccaattgt tttactattt
    16561 gccattagta atttgaatgc aaactttcaa gcaatcgcca cgggcgaggc accgcagttt
    16621 attttcacgc ctaatttttg ggattatttt ctaagtgtta ctggatcagg aattactggc
    16681 gccattgtga ttttatcttt gttgagtaag aaaacaacat taaagaatct cggtcgaaca
    16741 gccgctagtg gaaccttgtt ttcagtttcc gaaccaattg tttttgggat accggtggtg
    16801 atgaatccat acttttttat cccctttgtg attgggactc cgattttagg ggttattcaa
    16861 tggtatgttt tcaaacttgg ttgggtttca ttaccgactt attttgtggc agatttacca
    16921 ctaccatttt cgagcttctt ggcaacgatg gattggcgtt ctttgatttt gaccgccgca
    16981 accattgcct tagcaacttt gatgtactgg ccgttttata aagcatttga gaaaagttat
    17041 gtagaaaaaa ataatgatga taagtatcag gatctagatt tggattttta gaaaagagga
    17101 ataaaattgc taaaatcttt ttttaaaata ttttgcggaa actttatttt gggcttagca
    17161 tacgccaaac tgatggttcc aaatgagatc attaatggtg gtgtgacgag tctttcgttg
    17221 atatttagta atctgacttc cctcaatatc accttattta caaatgtcat caccgtgaca
    17281 ttactggcag tttcattgct ctttttaggg aaatttttct ttttctcttc cgtcgttagc
    17341 agtgtttctt atttgctgtt tttcaatctt ttctaccgct tgccattctc actacataca
    17401 aatgttgtgg ttgattttct tttggcagta ttgctgattg ccatcggtta ttattgctgc
    17461 ttgtcagaaa attcttctac agcaggttta gatgtatttg cggttatttt gaataaaaaa
    17521 atccccaaaa tctcagtggc tgttttcttg aggtatatta atatcgcagt tttagttttg
    17581 gggctgtttt catatggaat caaggccgtc attttagggg ttttatttag ttttagtttt
    17641 actaaagtaa tggatctttt gatggaaaaa ggtgcggatc cccgcaaatt tttt
//
LOCUS       DACGLE010000056        17421 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0534
            SAMEA3304449-rid9560453.denovo.060, whole genome shotgun sequence.
ACCESSION   DACGLE010000056 DACGLE010000000
VERSION     DACGLE010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304449
            Sequence Read Archive: ERR985064
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17421)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17421)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702225.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702225.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 20:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,045
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 27 of 67
            Pseudo Genes (incomplete)         :: 25 of 67
            Pseudo Genes (internal stop)      :: 19 of 67
            Pseudo Genes (multiple problems)  :: 4 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17421
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304449-rid9560453.denovo.060"
                     /strain="VREN0534"
                     /db_xref="taxon:1352"
     gene            282..521
                     /locus_tag="IXT20_001981"
     CDS             282..521
                     /locus_tag="IXT20_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4860419.1"
                     /translation="MKRILFISLSNLLLLTACGTSASKNIPSDTQISNSIDSTISTNE
                     KNSPVETSDIKVSTSAKNRVKKLSARLLYFKQTLN"
     gene            complement(883..1143)
                     /locus_tag="IXT20_001982"
     CDS             complement(883..1143)
                     /locus_tag="IXT20_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4860420.1"
                     /translation="MNNETIILGKKYICQPIGLKQKVVGEVISKLENCVVICVDEYHD
                     VDRNEILEKCGKVVAKYTHVYELETTEYLYNYSNKYEHTLVS"
     gene            complement(1328..1825)
                     /locus_tag="IXT20_001983"
     CDS             complement(1328..1825)
                     /locus_tag="IXT20_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HAQ4860421.1"
                     /translation="MWTEKQKKDAYEILLLQQQGLLEAEDNWLANLANSSALLNETLP
                     ETVFAGYYLFDGNELILGPFQGRVSCTRIKMGKGVCGESAEKQETLIVDDVKTHANYI
                     SCDSAAQSEIVVPMVKEGNLIGVLDIDCGVTKGYDEIDQAFLEKYVDLLLANFNINAF
                     SFINN"
     gene            complement(1955..2665)
                     /gene="budA"
                     /locus_tag="IXT20_001984"
     CDS             complement(1955..2665)
                     /gene="budA"
                     /locus_tag="IXT20_001984"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HAQ4860422.1"
                     /translation="MTEKILYQHGTLGALMAGLMDGTETIAHILEKGTLGLGTLHGLD
                     GEVIFLDGVAYQGRSDGSVAQLDGSELTPYAAITDFTPDTSFSVSKTADGEQLKKDIL
                     VREAGENLFLAVKITGLFKNMHIRIMPKQEKPYRRLAKISESQPEFQQSNVQGTLVGF
                     FTPELFQGVAAAGFHLHFIDDTHTFGGHVMDFEVAEGKVEISRISSLVQHFPVDDPTF
                     VHETIDYADLAAEIQQAE"
     gene            complement(2678..4342)
                     /gene="alsS"
                     /locus_tag="IXT20_001985"
     CDS             complement(2678..4342)
                     /gene="alsS"
                     /locus_tag="IXT20_001985"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase AlsS"
                     /protein_id="HAQ4860423.1"
                     /translation="MDEKMKQGSKSVVESLINHHVDYVFGIPGAKIDGVFNELEDQGP
                     ELIVTRHEQNAAFMAQAIGRITGEPGVVIATSGPGASNLATGLVTATAEGDPVLAIAG
                     QVKRSDLLKLTHQSMDNAALFQPITKYSAEIQDPETISEVIANAYRMAKSSKKGASFI
                     SIPQDVVDAPVQGNVIKPLSDPKLGSASADDIRYLAERIREAKLPVLLVGMRGSSEKE
                     TLAIRQLVGKTALPVVETFQAAGVISRELEAHFFGRVGLFRNQPGDMLLKRSDLVIAI
                     GYDPIEYEARNWNAEKDARIIVIDEAPAEIDPFMQPERELIGDISATLDLLTGSLEPQ
                     QVSEDAKEYLASLQAELTERDIVQSKGEAGILHPLEVINTLQSKVTDDMTVTVDVGSH
                     YIWMARHFRSYEPRHLLFSNGMQTLGVALPWAISAALVRPNTQIVSVSGDGGFLFSAQ
                     DLETAVRKKLNIVHLIWNDGHYNMVEFQEKMKYQRASGVDFGPVDFVKYAEAFGAKGI
                     RATSVEELEKALEEGFATEGPVIIDIPIDYRDNEKLGETILPDQFY"
     gene            complement(4547..5209)
                     /locus_tag="IXT20_001986"
     CDS             complement(4547..5209)
                     /locus_tag="IXT20_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HAQ4860424.1"
                     /translation="MKEQRFDKNVYMEFFYWLYQSLASSLCFWCVNILYIAAAFLLSI
                     DGRNVFSFIFSLLFIGPGCIVMIGMTEELAKEKSFSPVRLFIKTFRQFWLKGFFYWLF
                     AWIVSVIMIFDCFFFIRFSYGKWLIPLFVLLACLSVSFSINYWYFQVRNPASKPNQVL
                     RIAFYYTLKKWYVSLLDFLLLTSLFLFFFVKPQWCILLGPSIVFGLIYFNNRKLMRTM
                     DL"
     gene            complement(5219..6034)
                     /locus_tag="IXT20_001987"
     CDS             complement(5219..6034)
                     /locus_tag="IXT20_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAQ4860425.1"
                     /translation="MPFIQWLGMILLIVLALLTIIPFLWMVSASFKANNEVFTIPIQW
                     IPKTWHLENYLVIWSRIPLVTFFKNTAFLSVVITLIQLFTSSFAAYGFSKLNFKGRDI
                     LFLAYIGTIAVPWQSYMIPQFIMMRKVGLTYTLWSLVLLQAFNAFGVFLLKQYYSSIP
                     DSLCESARIDGLSEWGIYRKIILPLTKPAIASLTIITFVNTWNDYMGPFIYLSSTENK
                     TIQLGLKMFIGLFDAEYALIMAASVVSVLPVAFIFLAMQKYFVEGIASSGMKE"
     gene            complement(6296..6739)
                     /locus_tag="IXT20_001988"
     CDS             complement(6296..6739)
                     /locus_tag="IXT20_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator
                     substrate-binding protein"
                     /protein_id="HAQ4860426.1"
                     /translation="MLDPVDTEKYETILLPRKEKWGLLILENSVLAKKDVITKEDFAN
                     VPLLISKRPEIQRMFADWLGIKKEQLLITGTYNLIFNVFALIENQVGAALVIEGAVSH
                     LSKRVTFVPLDPMIETNCMLVWKKNRILSPVVNELIHRFKEAFEQ"
     gene            complement(6873..7211)
                     /locus_tag="IXT20_001989"
     CDS             complement(6873..7211)
                     /locus_tag="IXT20_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAQ4860427.1"
                     /translation="MYQVKKSRSIAMELRLLKYFWTIAEEGTISQAAEVLHLTQPTLS
                     RQLKELEEELGTELFVRENRKMILTEAGYFLKKPSRRNFRFNESNHSRIREQKKTIIQ
                     RPIYDRLCGS"
     gene            complement(7199..7576)
                     /locus_tag="IXT20_001990"
     CDS             complement(7199..7576)
                     /locus_tag="IXT20_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAQ4860428.1"
                     /translation="MNIKQVSEEKGISADTLRYYERIGLIPPVNRTKGGIRDYTEEDL
                     KWVDFTICMRNAGLSIESLIEYIRLSSEGEATVFERRKLLIEESELLTKKIAEMQECL
                     ERLQGKIKKYDRVLSRNKMECIK"
     gene            7800..8993
                     /locus_tag="IXT20_001991"
     CDS             7800..8993
                     /locus_tag="IXT20_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ4860429.1"
                     /translation="MTTYQRKLLLSTSAGIALENMDIMFLAFSLSSMITSFHISGTQA
                     GFISTITNLGMLIGGIFFGIIADKYGRVKVFSQTVLLFSIASLLMYFASNIYLVYLFR
                     FIAGVGAGGEYGACMSLISESFSKKQIGRASSVAGIGAQVGAALAAILAAVIIPTFGW
                     KMLYVVGVLPVLMVLFIRRGLTEPKEFKSVQSAEKKTKLSRLFESKHLAWQTCGLSIM
                     VTVQIAGYFGLMNWLPSIMQNRLGLSVSGSSLWMVSTILGMSLGMMTFGIIMDKLGPK
                     LAFSLFLICSSLSVYLLVLAHSQWSLVAAAVIVGYFINGMYGGYGAVISSLYPTEIRA
                     TANNFIMNLGRAVGGFSSIVIGFLMDHYSLTAVILFLSGIYLVSLLVLTTLTGVTALR
                     ESLQH"
     gene            complement(9159..9581)
                     /locus_tag="IXT20_001992"
     CDS             complement(9159..9581)
                     /locus_tag="IXT20_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAQ4860430.1"
                     /translation="MQTFLTKVSQKKIKMIHLLLETERWCSIEELQNELKVSSKSILN
                     YLTDLEELFQKYPDKVILKNENNRRFSMEKQENFPIYIIYLHFYRKSYNYHLIEFMYQ
                     HPEKNLEDYADAQYTSVSTVFRYAKLLVKYFEMTDVKE"
     gene            10171..10626
                     /locus_tag="IXT20_001993"
     CDS             10171..10626
                     /locus_tag="IXT20_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4860431.1"
                     /translation="MTEAESALSQAEANPTKENYATASAAIQALPENKQELTSRLAAI
                     DSTIKAKEAEEERKKQEEIARQQAEQAQQEAIAQQQAEQARQQAAAEQASQQAQAAAP
                     EQNNEQTVYVTPTGSKYHTHKCGNGTYTPATLSEAQARGLTPCSKCYGN"
     gene            complement(10788..11960)
                     /locus_tag="IXT20_001994"
     CDS             complement(10788..11960)
                     /locus_tag="IXT20_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAQ4860432.1"
                     /translation="MNKSRIRILDIFMAIILVVGIGIFSYPFVEDSLNDFLAQQMIIH
                     YQKQASNKNSEEIKKQQEKMTKKNQQLAEQNVSPGIGSFNQAVDAKALKDLPSNAFFM
                     EHMLGVIEIPKINVSLPIFDQTTEIFLQKGTSLLEESSYPTGGKNTHAVLSGHRGLPE
                     AKLFTDLPKLKKGDQFFIQINGKTLAYQVEKIQVVLPDEVDSLGIQKGRDLVTLLTCT
                     PYMVNTHRLLVTGHRIPYQAKEAKKAIQGIDQWKKWKFFIWFIGILLGSIGLVWLLIA
                     YLDSLAIAKRNYPLSFYVKNTNGRPIEGMVFSVKTLNGKHYITREKVPFVKASDEYGL
                     VRFSDLKGRNYRLQHEELLLKIHVKHKHSKQFSMKLKKGRYKLRKEKEVYYLIEKE"
     gene            complement(12166..13595)
                     /locus_tag="IXT20_001995"
                     /pseudo
     CDS             complement(12166..13595)
                     /locus_tag="IXT20_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306286.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
     gene            complement(13592..15094)
                     /locus_tag="IXT20_001996"
                     /pseudo
     CDS             complement(13592..15094)
                     /locus_tag="IXT20_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345213.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
     gene            complement(15091..17418)
                     /locus_tag="IXT20_001997"
     CDS             complement(15091..17418)
                     /locus_tag="IXT20_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAQ4860433.1"
                     /translation="MKMIKKTQNKIQRICQKHFVSRLKAVSKRKYLHPIMISIILIFT
                     FLGSLSSIYVNVYTETSNFANQTTEETNSPPYSEIQSIESTNQSSNNETTSTTSSTQL
                     IDPTEKEITAAKAAGEKAYSETGRSQTFSTVAATANFNGHTIPDGYTVIYAKDGVIVA
                     VNGSMSADKTALQGVTKAIAFIDNRPDIHNLFIGSTVNGTTITFPKSIYMGVATAGNT
                     IDSVTPNADKSYSFTPSTWGGLPAGGGMSTFSIGIQLANDIPFTGYSIKNGQIVDGKG
                     NVSNFPTYQAATVQVNANRTGTFSYTVNGGAAKIGTYGTTLSGIHVGDKVVITPTAVT
                     GYSFAQTNGSFTALESNVDTVTYTGKQTPITIKYQDYFGQMIAPSKIMNLTYGSAAQD
                     LTTNTPTINGYTFTAVSATETKNQSAMSVLASLDTNGNVIVKDANGKQISEVILYYKT
                     NATVSINANGSKYYDGLSVVPIVKYAFNDKTESTSLLNNDLSIDQITWNPTDFKAMNE
                     KGVEVTAPTEIGTYTSWQLTKSGLAKLAARTNYLFTIVQTSDVYTIKQISGAVELSGS
                     KTYDGKAGVPSIHVKLAEGVTSSLTPVQVALSSMDYTADAQSAKNMVNAGSYTIKLTN
                     SGIDKVKAANPKLSFTDIANTSGTYTIYKANAVITVDDAAFNYDAQSHSISVGNLHVT
                     GVVLGESLDYTLTVNSRTDIGSQIVTISLGEGSVNNNYTIIATDTAKLTINPTLLLPS
                     TGGIGLLPFVFLGLIFIFSGFFYFIHRKKKAGEQR"
BASE COUNT         5024 a         3461 c         3036 g         5900 t
ORIGIN      
        1 tatcctccta gcaactattt aaatacccaa aaaaattacc tctagataat atcataccta
       61 cgttttcact tataaatttt ttcataaaaa aagcgtttag aggcataatc gttaatacag
      121 atggtaacca atgtatctgg tttgaactgg gatatcaccc tctccatata tgtattacta
      181 agactaatag atactataaa agacttgata gttttgcatt gattttgcct aatactgagc
      241 ataggtgtat gctaaacgat actataagga gtgtgtacat aatgaaaaga attctgttta
      301 tcagtttatc aaatttattg ttacttactg cttgtgggac ttctgcatca aagaacatac
      361 cttcagatac gcaaatttcg aatagtatcg attcaacaat ttctacaaat gagaaaaatt
      421 ctccggtaga aacttccgat ataaaagttt ctacctctgc aaaaaacagg gtaaagaaac
      481 tgtcagcacg tctcctttat ttcaaacaaa ctctgaatta gcttcttcat cttcttcagc
      541 aaataatgat gatagtttcg cgtttactcc taactaaatg caacctttac aaatgcttta
      601 cgataacaag cctgagctaa atgatcctga tatcagtttt gcattttttg aaatgattgg
      661 atcggatgat ctttttaaaa ctactagttt gtctatccga aaataggaca gttctggcag
      721 tataggtttt tatcgagttt ctccaccagg cctcgttaca gagacagatt ctctaggcga
      781 accttttgaa taaatcaaaa atcatgaaca atcactgcca actaaataaa gtgccttcaa
      841 aagcttggtc ttctcgcttt tgaaggcact ttatttaaat ctttatgaaa ctaaagtatg
      901 ttcatatttg ttgctatagt tatatagata ttctgttgtt tccaactcat aaacgtgagt
      961 atattttgcc actacttttc cgcatttttc taatatttca ttgcgatcga cgtcatgata
     1021 ttcgtctacg cagatcacaa cacaattttc taatttactg attacttctc caaccacttt
     1081 ttgtttcaag ccaataggtt ggcaaatata tttttttcct aaaatgatag tttcgttatt
     1141 catatttcat ccatccttaa tgtttttcca ttatttaaaa tgataatacc attaaaatat
     1201 gattatgaaa atcaataaca cttgtgattt tataaaaaaa taaaaaaacc gaaaaacaga
     1261 aatgcccact gtctttccgg ctctttttat tcttgatttc tcttcttttt tgtttcaata
     1321 gacgatttta gttattaata aatgaaaaag catttatatt aaaatttgct aataaaagat
     1381 ctacatattt ttctagaaat gcttgatcga tttcatcata ccctttcgtt actccgcaat
     1441 cgatatctaa aacaccgatt aaattacctt cttttaccat tggcactacg atttctgatt
     1501 gtgcggctga atcgcaggaa atgtaatttg catgggtttt gacatcatca acgatcaatg
     1561 tttcttgttt ttcagctgat tctccacaaa ctccttttcc catcttgata cgtgtgcaag
     1621 aaactcgccc ttggaacggt cctaaaatca attcattacc atcgaataaa taatatcctg
     1681 caaagacagt ctcaggcaaa gtctcattca acaatgcaga tgaatttgct aaatttgcta
     1741 accagttatc ttctgcttct aataatcctt gttgctgtaa taataaaatt tcgtatgcat
     1801 cttttttctg tttttctgtc cacatgataa aaaccgctcc tttttgatct atggagaaga
     1861 ttatagctca gtttttttgt aaacgctagg tttcctcctc aaaaaaacga gatacgtcag
     1921 gttaatcacc ttgaacgcat ctcgctgtct ttttctattc tgcttgttgg atttctgctg
     1981 ctaaatcagc ataatcaatc gtttcgtgaa caaaagtcgg atcatcaact ggaaaatgct
     2041 gtacaaggga cgagatgcgg ctaatctcaa cttttccttc agccacttca aaatccatta
     2101 catgtccgcc aaatgtatgt gtatcatcga taaaatgcaa gtgaaatccc gctgcagcaa
     2161 ctccttggaa taattcaggc gtaaagaacc cgactaatgt tccttggaca ttgctttgtt
     2221 ggaattctgg ttgagactct gaaatttttg ctagtcttct gtaaggtttt tcttgcttcg
     2281 gcatgatacg gatgtgcata tttttgaata atccagttat cttcactgcc aaaaataggt
     2341 tttccccggc ttctcttact aatatgtctt ttttcaattg ttctccatct gcagtttttg
     2401 agacagaaaa actagtatct ggcgtaaaat ctgtaattgc tgcatagggg gtaagttcac
     2461 ttccatctaa ttgggcaact gagccatctg atcttccttg ataagctacg ccatctaaaa
     2521 agatgacttc accatcaagc ccatgcaatg ttcccaatcc cagagttcct ttttctaata
     2581 tatgagcaat cgtttctgtt ccatccatca agccagccat caaagctcct aatgtcccgt
     2641 gctggtataa gattttttca gtcatgtcga cacttcctta atagaattga tctggtaaga
     2701 ttgtttctcc caatttctca ttatcacggt aatcaatcgg aatatcaata attacgggtc
     2761 cttctgtcgc aaatccttct tctaaagctt tttctaattc ctctacactt gtagctcgga
     2821 ttcctttagc gccaaaagct tctgcatatt tcacaaaatc aactggacca aagtctacac
     2881 ctgacgcccg ctgatatttc attttttctt ggaactctac catattgtag tgtccatcat
     2941 tccaaatcaa atgaacgata ttcaattttt tacgtaccgc cgtttctaaa tcctgggcag
     3001 agaataaaaa tcccccatcg ccggaaactg atacgatttg tgtatttgga cgcaccaatg
     3061 cagcagaaat cgcccatggc aatgcaacac caagcgtttg cataccatta ctgaataaca
     3121 aatgacgagg ttcataactt ctaaagtggc gtgccatcca aatataatga ctacctacat
     3181 ctacggtaac agtcatatca tctgttactt ttgactgaag cgtgttaata acttctaaag
     3241 gatgcaagat acctgcttct ccttttgatt gaacaatgtc ccgctccgtt aattctgctt
     3301 gaagagatgc taaatattct tttgcatctt ctgacacttg ttgaggctct aatgaacctg
     3361 tcaataaatc aagagtggct gagatatcac cgatcaattc tctttcaggc tgcataaaag
     3421 gatcgatttc agctggcgct tcatcgatca caatgatccg cgcatctttt tccgcattcc
     3481 agtttcttgc ttcatattcg attggatcat agccaatagc aataactaga tcacttcgtt
     3541 tcaaaagcat atctcctggt tggtttctaa ataaacccac acgaccaaag aaatgcgctt
     3601 ctaattcacg tgagatcact ccagctgctt ggaatgtctc tacaacaggc aatgctgtct
     3661 ttccaactaa ttgcctgatg gctaacgttt ctttttcact tgatcctcgc atacctacta
     3721 atagtaccgg tagttttgct tctctaatgc gttcagctag gtaacggata tcatctgcag
     3781 aagcagaacc taatttagga tcgctcaatg gcttgatgac attcccttgg acaggtgcat
     3841 ccactacatc ttgcgggata ctgataaagc tcgcgccttt ttttgaactc ttagccatac
     3901 gataagcatt ggcaatcact tctgaaattg tttctggatc ttggatttct gcactgtatt
     3961 ttgtgattgg ctggaacagt gctgcattat ccatgctttg atgggtcaac ttcaatagat
     4021 cgcttcgttt tacttgtccg gcaattgcta aaactgggtc accttctgct gtcgctgtta
     4081 ccaaacctgt agctaagtta ctagcaccag gaccacttgt tgcgataact actcctggct
     4141 ctcctgtaat acgtccgatt gcttgtgcca taaatgcagc attttgttcg tgacgagtca
     4201 cgattaattc aggaccttga tcctctaatt cattaaatac accatcgatt tttgcacctg
     4261 gtataccgaa tacataatca acatgatgat taatcaaact ttctactaca gatttcgaac
     4321 cttgtttcat tttttcgtcc attgaacatc gtccttttta tgctttattt ttaattatta
     4381 gttagaaata atcacaaata attgatacag tttctaaacc tgtttcattc aaaagtatag
     4441 cacgttttat ttcatttgtt tagccatatg tttcaaaaaa tgaaaaagaa tgacagtttt
     4501 tacataaaaa ctgtcattct taattgaaag tttcaattta taaagtctac agatccatag
     4561 ttctcatcaa tttacgattg ttgaagtaaa tcaaaccaaa tacgatagaa ggacctagta
     4621 gaatacacca ttggggcttt acgaaaaaga agagaaacaa acttgtcaat agtaaaaaat
     4681 ctagtaaact cacataccat tttttcaacg tatagtagaa cgctatccgt agaacttgat
     4741 ttggtttcga cgctggattt ctcacctgaa aataccagta gttgatgcta aaggaaactg
     4801 ataaacaagc aagtaaaaca aacaacggaa tcaaccattt tccataagaa aaacgtataa
     4861 aaaagaaaca atcaaaaatc attatcacac taactatcca agcaaatagc caataaaaaa
     4921 atccctttag ccaaaattgg cgaaacgtct ttatgaacaa acgtactggt gaaaaacttt
     4981 tttcttttgc taattcttct gtcataccta tcatcacgat gcaacctgga ccaataaaaa
     5041 gtaaagaaaa aataaaggaa aagacattcc ttccatctat cgacaaaagg aacgcagctg
     5101 ctatatacaa gatattgaca caccaaaaac aaaggctact tgccaatgat tgatacaacc
     5161 aataaaagaa ttccatataa acatttttgt caaaacgctg ctctttcata atcacctcct
     5221 attccttcat tcctgatgaa gcgatccctt ctacaaaata tttttgcatc gctaaaaata
     5281 taaaagcaac aggaagaaca gaaacaactg aggcagccat gattaatgcg tactctgcat
     5341 caaatagccc aataaacatt ttcaacccta actggattgt tttattttcc gtagaagaca
     5401 aataaatgaa tggccccata taatcgttcc atgtattaac gaatgtgatg atcgtcaaac
     5461 tggcaatcgc cggttttgta agaggtaaaa tgatcttgcg atagattccc cactctgata
     5521 agccatcgat ccgcgcactc tcacataaag aatctgggat actactgtag tattgcttca
     5581 acaagaacac accaaaagca ttgaacgctt gtaatagcac caacgaccac aacgtatatg
     5641 ttaaacccac ttttctcatc ataatgaact gcgggatcat ataagactgc caagggacag
     5701 caatcgtgcc gatatatgct aaaaataaaa tatcgcgccc tttaaaattt agtttagaaa
     5761 acccgtaagc agcaaaacta gaagtaaaca actgaatcaa cgtgatcaca acgcttaaaa
     5821 aagctgtatt cttaaaaaat gtcactaaag gaatacgaga ccaaataacc agataattct
     5881 ctaaatgcca agttttaggg atccattgaa tcggaatcgt gaacacttca ttatttgctt
     5941 tgaatgaagc agaaaccatc cataaaaaag gaatgatcgt caataaagct aatacaatca
     6001 gcagaatcat acccaaccat tgaataaagg gcatgttttt tgaacgaatt tttgcttttt
     6061 ttctttcatt tcgaacacct ccaaagttat aatcttcatt tctttgtaat gattacaatc
     6121 cagaacaacg gttattatag ctgattcatt tccgttcttc ttatgtttgt aagcgcttat
     6181 ttaacaagtt atgtaaatct ctttattttt tttgatttaa aaatgaaaaa atacaaaagc
     6241 aagactgtca tacgtctcgc ttttgtattg aaatttcttc gattagagat acttattact
     6301 gttcaaaagc ttctttgaaa cggtgaatca attcattgac tacaggtgat aaaatccgat
     6361 tctttttcca gactagcatg cagttcgttt caatcattgg gtcaagcgga acgaatgtca
     6421 ctcgctttga aagatgactt acagcacctt ctatgactaa agcagcacct acctgattct
     6481 caattagcgc aaatacattg aaaatcaaat tgtacgttcc tgtgatcagc aactgctcct
     6541 tttttatgcc taaccagtca gcaaacattc gctgtatttc tggacgcttt gagatcaaaa
     6601 gaggtacatt cgcaaagtct tcttttgtta tcacgtcttt ttttgctaaa acagaatttt
     6661 ctaaaatgag caatccccat ttttcctttc ttggtaacaa aatcgtttcg tatttctctg
     6721 tatctactgg atctagcaaa atcgctaaat ctaacaggct tttttctagt cactccgtga
     6781 tatcatcact tgttccgctg tggatatgaa attgaacgct gggataatca cggatcaact
     6841 tttctagtaa tagtgccaat gtattcgaat tatcagcttc cacacagccg atcgtaaatc
     6901 ggccgctgaa taattgtttt tttctgttct cgaattcttg agtggtttga ttcgttaaat
     6961 ctaaaatttc ttctgctcgg cttttttaga aaataacctg cttctgtcaa gatcattttg
     7021 cggttttcgc gtacaaacag ttctgttcct agttcctctt ctaactcctt cagttgtcgg
     7081 cttaaggttg gttgtgtaag atgtagaact tctgcggctt gagagattgt tccttcttct
     7141 gcaattgtcc aaaaatattt tagtaagcgt aactccatag cgatgctcct tgattttttt
     7201 acttgataca ttccatttta tttctagata acacacgatc atatttcttg atttttcctt
     7261 gtaaacgttc caaacattct tgcatttcag caattttttt cgtcaaaagt tcactttctt
     7321 caatcaaaag cttacgccgc tcaaatacag ttgcttctcc ttcgctagat aggcgaatat
     7381 attcaatcaa agactcgatc gataatccgg catttcgcat acagatcgta aagtcgaccc
     7441 attttaggtc ttcttctgta taatcacgga ttccgccttt tgtccgatta actggtggaa
     7501 tcaacccgat tcgttcataa tagcgcaatg tatcggccga aatgcccttt tcttcactga
     7561 cttgtttgat attcatctga tcactctttc tcttaatttc ttgccacttt acttatgtat
     7621 tttatttatt ctacaacttt aagtgtactc caagtcaata ctcatttcta ttaaaactaa
     7681 gctggattat ctatatcggt tgtttgtgta ttatttcaaa taaaaaagtc ttgttttttt
     7741 attctttttt ggatatactt ttaacaattt agggaaattt taggaaaagg ggtataaaaa
     7801 tgacaacgta tcagcgaaag ttattattat cgacatcggc gggaattgct ttagagaata
     7861 tggatattat gtttttagct ttttcattat cttcaatgat tacttctttt catattagtg
     7921 gtacgcaagc gggtttcatt tcaacgatca ctaatttggg gatgctcatt ggaggtattt
     7981 tttttggcat catagctgat aaatatggac gtgtgaaagt attttctcaa acggtccttc
     8041 tgttttcgat tgcttcttta ttgatgtatt ttgcttcaaa catctacttg gtttatctct
     8101 ttcgctttat cgcaggtgta ggagcgggag gagaatacgg ggcgtgtatg tccttgattt
     8161 ccgaaagttt ttcaaaaaaa caaattggac gagcttcttc ggttgctggt attggtgcgc
     8221 aggtcggtgc ggctttggct gcgattttag ctgctgtgat cattccaacg tttggttgga
     8281 agatgttgta tgtcgtaggt gtactgccgg tgttgatggt cttgttcatt cgtcgcggct
     8341 tgactgaacc aaaggaattc aaatctgtac aatctgccga aaagaaaacc aaacttagca
     8401 gactgtttga atcaaaacat cttgcttggc aaacatgtgg ccttagtatc atggtaactg
     8461 tccaaattgc cggctatttt gggttaatga attggctgcc ttcaattatg caaaatcgat
     8521 tagggctttc ggtttctggg tcttcattgt ggatggtcag tactatttta ggcatgtctt
     8581 tggggatgat gacttttggc ataatcatgg ataaattggg gcctaaacta gccttttctt
     8641 tgtttttaat ttgttcatcc ttatctgtct atctacttgt gttagcccat agccagtgga
     8701 gtttggtagc agccgctgtg atagtcggct actttatcaa tggcatgtat ggtggatatg
     8761 gcgcagtgat tagtagtttg tatccaacag aaatccgcgc gacggcgaat aatttcatta
     8821 tgaaccttgg acgagctgtt ggtggttttt cttctatcgt catcggtttc ttgatggatc
     8881 attatagttt aactgccgta atcttgtttt tgagcggtat ttacctagtc agtctgctgg
     8941 tcttgacaac tcttactggt gttacagcat taagggaaag cttgcagcat tgatagttga
     9001 gtctgtaaat gacaagaaag aggtgtggca aaagcttgca tctgcggtaa taagaccagc
     9061 caaatcaaaa acatgagaca ctgtttccgt ttggctggtc gtattacttg atgcagggca
     9121 gctttttcaa ctgctaattt ccttctatct ggcggctact attctttgac gtccgtcatt
     9181 tcaaagtact ttactaataa cttagcataa cgaaaaactg tggatacact cgtatattga
     9241 gcgtctgcat aatcttccaa atttttctca ggatgttgat acatgaattc aatcaaatga
     9301 taattatatg attttctgta gaaatgaaga taaataatgt agattgggaa gttctcctgc
     9361 ttttccatag aaaagcgccg attattctca ttcttcaaaa tgaccttatc aggatatttt
     9421 tggaacaatt cttctaaatc tgttagataa ttcaagatag acttacttga taccttcaat
     9481 tcgttttgta gttcttctat tgaacaccaa cgttctgttt caagcagtaa atgaatcatt
     9541 ttaattttct tttgagaaac ttttgttaaa aaagtctgca tattttctcc tcctgtatct
     9601 actttactat actacataca agaaaaaaaa cggatacaaa tatcatattg tcacaccgtc
     9661 aatagtgcat aaacctgtta ttatttctct ttttttccta agtattctat tgatatgaat
     9721 catttaatcg tttattatat aaatgtcaat aatctaataa taaaataacg tacatatttt
     9781 attattgcat acttaataac agaactatag caaaaaatcc cgtgtatcaa aacgggatta
     9841 gattaagttt cctcaatcac tattcataac acaattatta taacaagtaa gtaaacaatt
     9901 gaaaaggaca tttattgaaa acggttgttt tttacttaag gaggaaattt agtgaaaaaa
     9961 ttagttgcat tgtttttggt cgcaggaagt ttgttgagtg ggtgtggtgc tactacgtct
    10021 actactcaaa gcacaagttc agctcaggct tccagcagca gtaccacgat ctccgaaagt
    10081 acggcagatt catcgaaaga acaagaacaa attgaaaaga aaaaagaaga agagaaacaa
    10141 aaagcggaaa aagctgcact agaaaaaaaa gtgacagaag cggaatctgc tttgtcacaa
    10201 gctgaagcaa atcctacaaa agaaaactat gcaactgctt cagcagctat ccaagcgcta
    10261 cctgaaaaca aacaagagtt gacaagccgt cttgctgcaa ttgattccac aatcaaagca
    10321 aaagaagcag aagaagaacg gaaaaagcaa gaagaaattg cacgacaaca agcagaacaa
    10381 gcgcaacaag aagcaatcgc acagcaacaa gcagaacagg cgcgacagca agcagcagct
    10441 gaacaggcct ctcagcaggc acaagccgca gctcctgagc aaaataatga acaaactgtc
    10501 tatgtcacac cgaccggttc aaaataccat acacataaat gtggaaatgg tacgtatacc
    10561 cctgccactt taagcgaagc tcaagctcgt ggtttaaccc cttgctccaa atgttatgga
    10621 aactaattca aaagttatac tataataata aaattcttct gaaacataaa aaaatagaag
    10681 ctggcgttcc agatagtaaa ccaaaaatag cagtttacac gtccatttcg tcagcttctc
    10741 tcttttttca tttagtctgt tccctctgct tttttggaca tcttcattca ttctttctca
    10801 atcaaatagt acacctcttt ttcttttcgc agtttatatc ttcctttttt caatttcatt
    10861 gaaaactgtt tcgaatgttt atgttttaca tggattttaa gcaggagctc ctcatgttgc
    10921 aagcgatagt tcctgccttt taaatcagaa aatctcacta gtccgtattc atcagaagct
    10981 ttcacaaatg gaactttttc tctagtaata tagtgtttgc cgttgagcgt ttttacagaa
    11041 aagaccattc cttcgattgg tcgtccgttt gtgttcttta cataaaaaga taaaggatag
    11101 tttcgcttag cgatggccaa agaatctaaa taagcgatga gtagccaaac taagccgatt
    11161 gatccaagta gtataccgat aaaccaaata aaaaatttcc attttttcca ttgatcaatt
    11221 ccttggatcg ctttttttgc ctcttttgct tgataaggaa tacgatgccc cgtcacaagc
    11281 agtcgatgtg tattgaccat ataaggagta caggtaagca aagtcacgag atctctgcct
    11341 ttttggatac ccaaagaatc tacttcatca ggtaagacca cttggatttt ttctacttga
    11401 tacgccaatg tctttccatt gatctggatg aaaaactggt ctcctttttt caacttaggc
    11461 aaatctgtaa ataattttgc ttctgggagt ccacgatgcc cagaaagaac ggcatgggtg
    11521 tttttcccgc cagtcggata agaactttct tccaataatg acgttccttt ttgcaaaaat
    11581 atttccgttg tctgatcgaa tataggtaag ctgacattga tttttgggat ttcgatgact
    11641 ccaagcatat gttccataaa aaaggcatta ctaggtaaat cttttaatgc ttttgcatca
    11701 actgcttggt tgaaactgcc tatccctggt gatacgtttt gctctgccaa ctgttggttt
    11761 ttctttgtca ttttttcctg ctgttttttt atttcctctg aattcttatt cgatgcttgc
    11821 ttttgataat ggatgatcat ttgctgggca agaaaatcat tcagtgaatc ttctacaaaa
    11881 ggataagaaa agatcccgat cccaacgact aaaatgattg ccatgaagat gtccagtatt
    11941 cttattctgc ttttattcat catgtccacc tattttctaa tatgtatatg aagaggttgt
    12001 gaaatcagcg ccttgacctg agtattcgct caacaaacgg caaaatagtt cctcctattt
    12061 tgccgtttgt tgagcgaata ccaagagacc gaccacttgt cacaaccttt ctccccattt
    12121 gaatcactat cgtttattta tcgtatgatt tttgtttcca atagtttaag cttctgtttc
    12181 ttgatggcgt tttttgaagt agatcactgc tcctccaact aaagcagtac ctaccaagat
    12241 gaatacgatg atccccatac caccagttga aggcaagatt cctttaggtt ggttatcgat
    12301 cgttgtttca tcagaagttg ttggatcatc atctactgta aattcagtgt acgcagcttc
    12361 attcttaggt aatgcgtagc cttctggtgc ttttgtttct tgaaggcagt atgccccttg
    12421 tttcagccct tcgattgaaa atttcccttc tgaatcagaa atcaatttca cttcttccca
    12481 gtcagaacct gtgacttcat ctgaccaagc atatgggtat tctcctgagg caagatttgc
    12541 tatttttttc attaatttaa cagttggatc ttttgctctt agaatagaaa attctgcatc
    12601 ggctaatttt tcttgcgtgt tcacatcttg tttgatgaag tggaaatcac cgtagttttg
    12661 gacattttct acagtaatcg tgttatccgt agttgtattt tcccgattga ctacaccgtt
    12721 tggattccca agattggctt catctgcgcc atggatgata ccggttgttt gttgcaattt
    12781 tccgtttttt actataaaag tagtttcttt ttgtggattg gttaattgat aaccatctgg
    12841 tgcttttgtt tcaactaaag tataattgcc gtctattaat ccattgacac tgatggatcc
    12901 ttcagtatca gttgtgaatg tcaacgctga ctcagcagga tcatagagag cgacaccagt
    12961 gttttcatca aaaccggtca cgaattgatt ctctgaattt ttgatctgga attgcgcccc
    13021 tgctaactta tctacccctg aaaagttccc agctttctgg attttgatat tgctttcttt
    13081 gatgacattt ttagcatata ggtgaatcgt gtctaaaggt ttgtcagggt ctgatgaatt
    13141 cgggttataa aagggtaagc ttaacacgat atttggcgac gtttcgacac cgggctttgg
    13201 tgtttctaca aagacgtata cagcatcttt cccatcagat ttacctggaa gagtcaggtt
    13261 agctaatcct tcaccagaag tcgttacact tgcgaagggt gtagcgtctg ctggaactgc
    13321 tgtgtcttta cttggatagt acgtagcagt gatgtcatat acgttgaatt caacgccgtt
    13381 caacggttcg ctattcggaa atgtcaattc cgttccgttg tttatttgat tttctggtgc
    13441 tgaagtgaat ttcaatttat gcaaattaac cgtaacattt tctggtgtag gtgatacggc
    13501 ctagaaacct ctgcagttcc taggccgaga acgaaaggaa gcagcatgat agcagttcct
    13561 aaaaacgtcc atatttttgt tttgatgttt ttcattttat ttccctccta ttgtttcctt
    13621 tttgttgttt tttataaaac caagctgtcc ctgttgaaag gactagcaac aaccctaaaa
    13681 ttgagaaacc tccaagcatc tcttcgcttg tttttggaaa gcttcctcca tttttttcat
    13741 ttgatggacc atttgtctgc atctgaactg gcgtttttcc attattcgtc ggatttgctt
    13801 ttggttcgtt gatgatttcc aatggtatag gatgaagcgg atcataactg cctttttcaa
    13861 cttcaaagag aatggttgat tgactcaata tatatccttt aggcggttta atctccacta
    13921 gttcgtaact tccaaaagac aatccatgga cagaaaatgt tccatcttta ctggaagtaa
    13981 aaatcgttgc tcttgaatca tctttggcta cccaatgatt tatcccgttt tcttgcgcta
    14041 agtattggtc attttttttg actagaaatt gagcatctgc tagtttttgt ttcgtttgcc
    14101 gatccacttt gacaaattgt ttgcctcctg tttggatggc aagatgtgtt tctactgttt
    14161 gattgtcagt cgtcaaacgt acatcgttat caaatgtcgt tttttcactg ttttctttca
    14221 atgtcatttg gtaacggatc gttaattttt tcccagcaaa cgaactaaga tcttttccag
    14281 aatctgataa gacaaaacca tgttttgttt tttgtgtgtg aaatgtatga atcttgtctc
    14341 caccaatcaa aaaatcgatc gtttctggtt ctagccataa ctgaggatca gctgtatctt
    14401 tgatcgtata ggttcctatc tcgttgatgt tctcaggaat tgttacggtg atgagataag
    14461 gaactttatc cccatctgca aaatctgtct gcttattctc tactgttttt gttaatgtga
    14521 gtgcagcagg tattttttgt tcgctctttg gatacaagtg gatggtcgtc aatttatgat
    14581 tagatgaatc tgtcacagga agcacaacga caaatggtgc tgatttttct tttacttaat
    14641 ctgaagtctt tatttccgca aatcggtaaa cagcgtcacg accttgctca tctttgtccg
    14701 atgcactaaa agaagcaatg ccctcttcct cgtctactgt ttttgtcaca ttttctgcca
    14761 gtattttttc actatcactt ttttgtgcta gttttctttg tgcgtcttcc acactacttc
    14821 cttctgaccg cagcttgtaa aattcatccg agacatcata tatagcgaaa gtaacgccgt
    14881 tcaacccttt tactccttga aagttatccg ggatatttat tccgtcgttt tgactgatac
    14941 ttggcatctt atcgctttcg aacattcgat tatgcaaagt aaatgtaata ggtgattctc
    15001 tagctgacac agtcggagaa cctataaaaa agaccagtag gataaaaaag agaacagcaa
    15061 atatatccac acgtacattt tttgtttttt tcatcgctgt tcacctgctt ttttcttacg
    15121 atggataaaa taaaagaagc cagaaaaaat aaagatcagt cctaaaaaga caaagggcag
    15181 tagacctatc ccacctgtcg aaggaagcaa gagagtcgga ttgatcgtca acttagcggt
    15241 atctgttgcg ataatcgtat aattattgtt tacacttcct tcgcctaaac taatcgttac
    15301 tatttgtgat ccaatatctg tccggctgtt aactgttagg gtataatcca atgattcgcc
    15361 taagacaaca ccggtaacgt ggagatttcc aaccgaaatc gagtgacttt gggcatcata
    15421 attaaaggca gcgtcatcga cagtaatcac tgcgttagct ttatagatgg tataagttcc
    15481 tgatgtgttg gctatatcag taaatgacaa cttgggattt gctgctttga ctttgtctat
    15541 tccgctattc gttagtttaa ttgtatagct gccagcatta accatatttt ttgctgattg
    15601 tgcatctgct gtgtaatcca tactgctaag tgccacttgt actggagtaa gcgaactagt
    15661 gacaccctct gctagcttga catggattga cgggacacct gcttttccat catatgtctt
    15721 actgccactg agctctaccg caccgctgat ttgcttaatc gtatagacat cagatgtctg
    15781 cacgatagta aataaatagt ttgttctggc agctagttta gctagtccgc tttttgtcaa
    15841 ttgccaagac gtataggttc caatttcagt aggcgcagtt acttcaactc ccttttcatt
    15901 catcgctttg aaatcagttg gattccatgt gatttgatca atactgagat cattgtttaa
    15961 aagggatgtt gattctgttt tatcattaaa ggcatattta acgatcggta caacagaaag
    16021 accatcatag tacttactgc catttgcatt gattgatact gttgcatttg tcttataata
    16081 taggatgact tcagaaatct gttttccatt tgcgtccttt acgattacat ttccatttgt
    16141 atctaaacta gctaaaacac tcattgctga ttggttcttt gtttctgtcg ctgaaacagc
    16201 tgtaaaggta tatccgttga tagtaggcgt attagtcgtc aggtcttgtg cagcacttcc
    16261 gtaagtaaga ttcattattt tagaaggagc aatcatctgt ccaaaatagt cttgatactt
    16321 aatagtgatt ggtgtttgtt tgcctgtata ggtcaccgta tcgacgttac tttctagggc
    16381 tgtgaaagaa ccgtttgttt gtgcaaaaga atagcccgtt actgctgttg gagtgataac
    16441 cactttgtca cccacatgga ttccagataa tgtagtcccg tacgttccga tttttgctgc
    16501 tccaccattg actgtataag aaaaagttcc tgtccgattc gcattgacct gaaccgttgc
    16561 tgcttgatag gttggaaaat ttgaaacatt tcccttacca tcaacgatct gcccattttt
    16621 gatcgagtac cccgtgaatg gaatatcatt tgctaactgt ataccgattg aaaaagtact
    16681 catacctcct ccggcaggaa gccctcccca tgttgagggt gtaaaagaat aacttttatc
    16741 cgcattgggt gtaactgaat cgatcgtatt tccagcggta gccaccccca tatagatact
    16801 ttttgggaat gtgatagtag taccatttac tgtggagcca ataaacagat tatggatatc
    16861 tggacggtta tcaataaaag cgattgcttt ggttacgcct tgtaacgcag ttttatccgc
    16921 actcatcgat ccgttaaccg caacaatgac tccatctttg gcatatatca ctgtatatcc
    16981 atctggtatg gtatgtccgt tgaaattcgc agtggccgca acagtactaa atgtctggct
    17041 tctacctgtt tctgaataag ctttctcccc tgctgctttt gctgctgtga tttctttttc
    17101 tgtcggatca atcagttgag tcgaggatgt ggttgaggtt gtctcgttgt tgctagactg
    17161 attggtgctt tctatggact gtatttcaga atagggtgga ctatttgtct cctctgtcgt
    17221 ttggttagca aaatttgacg tttctgtata aacgtttaca taaatacttg ataagctccc
    17281 taagaaagta aaaatcaaga tgatcgaaat cataatggga tgtaagtatt ttcttttaga
    17341 tacagctttg agtctagaaa caaaatgttt ttgacatata cgttggattt tattttgagt
    17401 tttcttaatc attttcatcc c
//
LOCUS       DACGLE010000057        17351 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0534
            SAMEA3304449-rid9560453.denovo.061, whole genome shotgun sequence.
ACCESSION   DACGLE010000057 DACGLE010000000
VERSION     DACGLE010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304449
            Sequence Read Archive: ERR985064
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17351)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17351)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702225.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702225.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 20:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,045
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 27 of 67
            Pseudo Genes (incomplete)         :: 25 of 67
            Pseudo Genes (internal stop)      :: 19 of 67
            Pseudo Genes (multiple problems)  :: 4 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17351
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304449-rid9560453.denovo.061"
                     /strain="VREN0534"
                     /db_xref="taxon:1352"
     gene            complement(90..1109)
                     /locus_tag="IXT20_001998"
     CDS             complement(90..1109)
                     /locus_tag="IXT20_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAQ4860434.1"
                     /translation="MAITVKDVAKKAGVATSTVSRVINDHPSISETTKKKVRKIMDEL
                     GYVPNMTARNLGKRISSAIGVILPPLDSKERIGNPFYLEIMEAINEEARNYDMTTAIA
                     TAKNFELLLENVQRMHLQKQVDGFILVYSDSQDTVIDYLYQNHIPFTLIGQPYHHENE
                     IVYVDNDNQLLGKQATEFLIQNGHKNILFVTNTTHESLYFERYFGYQKAMMMAGLPAL
                     SSVVLEHPEDYLSFEKTLKENEATALVVIDDIFALRTMQLAQMYGYVIPETLSVISFN
                     NSIFSTLTHPYLTSIDIDISELGRVAMQKLRELIHEELSSGVRLVIPHRLIKRETVVP
                     LENPY"
     gene            complement(1131..2138)
                     /locus_tag="IXT20_001999"
     CDS             complement(1131..2138)
                     /locus_tag="IXT20_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HAQ4860435.1"
                     /translation="MDNPEISVVPVPETEIKKITLKNDYLEVVLLNYGARLHQIFAPD
                     KEGKSENVLLSYDRFEDVLTDKSFFGATVGPVAGRIRDASWGEHRLEKNCGSHHIHGG
                     TKGWSFQFWEVEVFKTPSSIGVVFYLKDEFSTYPGPITATITYRLTKNELEMTTSASS
                     LVETICNPTNHAYFNLSGNGTRDIYEHQLTVFLDGVLELDQEKLPTGNWTKKEKLPID
                     FRKSPTLQEILACYPDGLDDVFLLHHPRLSKTSLQLFEKHSGRQMTIATSNKSMVLFS
                     TTGFEADFSVNGKQMHSNYGLAIEPQEIPDIVHFPKFGSINLHPGQERISQTIYRFSA
                     Q"
     gene            complement(2131..2796)
                     /gene="pgmB"
                     /locus_tag="IXT20_002000"
     CDS             complement(2131..2796)
                     /gene="pgmB"
                     /locus_tag="IXT20_002000"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HAQ4860436.1"
                     /translation="MFKGVLFDLDGVITDTAEFHYRAWRKLGQEIGITIDHTFNEQLK
                     GVSREDSLSLLLAYGGKEHSFSKEEFAELAKRKNDYYLEMIQTIEPKDVFPGVVPLLD
                     SLKEEKIKIALASASKNGPFLLEKMGLTPYFDAIADPAEATNGKPAPDIFLLAAKAVG
                     LKAEECIGIEDAQAGIQAILSSGAQPIGVGRAEDLGEEIPLVPDTTFLTIDYLKEKWH
                     DHG"
     gene            complement(2789..5083)
                     /locus_tag="IXT20_002001"
     CDS             complement(2789..5083)
                     /locus_tag="IXT20_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HAQ4860437.1"
                     /translation="MKQIKRLFAIDPWKISTTQLDKKNLRLQESLTSIGNGYMGMRGN
                     FEETYSGDHHQGTYLAGVWYPDKTRVGWWKNGYPEYFGKVINALNFIAIDISINDQLI
                     DLASLEPEDFYWELDMKNGVLSRTFTITTSTNKVRISFERFLSIVKKESAYIYLTIEM
                     LEGSGTVKLVSKLDGNVQNEDSNYDEHFWNEIDRGTIEEIGYLTTKTIPNNFGIEQFT
                     VTAAMRHFVNGKSVIPDYHEDTLALHGCLSAELSQGETWSVEKEIIVLTSRDIPEAQQ
                     AEKAVSELTNQTNNYIQAKEEQTAAWAERWKLADVVIEGDEEAQQGIRFNLFQLFSTY
                     YGEDERLNIGPKGFTGEKYGGATYWDTEAYAVPLYLALAKPEVTKNLLKYRHNQLPQA
                     IHNAQQQGLKGALYPMVTFTGVECHNEWEITFEEIHRNGAIAYAIYNYTNYTGDTEYL
                     KNEGLEVLAEIARFWADRVHFSKRANKYMIHGVTGPNEYENNINNNWYTNTIAAWVLR
                     YTRENYLTYQKEAAITISEEELAKWKDIAENMYFPVDEELGIFVQHDTFLDKDLMPVS
                     DIPTSDLPLNQHWSWDKILRSCFIKQADVLQGIYFFNDTFTKEEKQKNFDFYEPMTVH
                     ESSLSPSIHSILAAELGMESKAVEMYQRTARLDLDNYNNDTEDGLHITSMTGSWLSIV
                     QGFAQMKTAHDQLRFAPFLPKTWKAYSFHINYRGRLLFISVTDEQVTFELLEGSPLEI
                     YVYEQSYSLADRLEITLRKEEANV"
     gene            5343..7508
                     /locus_tag="IXT20_002002"
     CDS             5343..7508
                     /locus_tag="IXT20_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ4860438.1"
                     /translation="MKKIFSFEFWQKFGKALMVVVAVMPAAGLMISIGKSIPLINPDW
                     TPLVTTGGVIENIGWAIIGNLHILFALAIGGSWAKERAGGAFAAGISFILINRITGAI
                     FGVTSDMLANEDAFTHTLFGTKIMVKGFFTSVLEAPALNMGVFVGIIAGFVGAMAYNK
                     YYNYRKLPDALSFFNGKRFVPFVVILWSTIVALILAVVWPNVQAGINNFGLWIAESQE
                     SAPILAPFLFGTLERLLLPFGLHHMLTIPINYTQLGGSYEILSGAQAGTQVFGQDPLW
                     LAWATDLVNLKGAGDMSQYEFVLHNWTPARFKVGQMIGSSGILMGMAFAMYRNVDPDK
                     KARYKSMYFSAALAVFLTGVTEPLEFMFMFAAVPLYVIYSVIQGAAFAMADILPLRVH
                     SFGNIELLTRTPLAIKAGLGGDLINFVLCVIAFGVVTYFLANFLIKKFNFATPGRNGN
                     YDNDSEETVVSNSGTGTADQQVVQIIHLLGGKENIKDVDACMTRLRVSVNDREKVGTE
                     EAWKRAGAMGLIVKDNGVQAVYGPKADVLKSDIEDLLQSGAEIPEPEMVSTGNGQAGS
                     KQYLGIEQELVSAASGEVIPLSEVKDPVFSQKMMGDGFAVIPTSREVVAPIAGKVTSI
                     FPSKHAIGLETKDGIEVLIHMGIDTVQMNQPAFEIAVKEGQEVGTGTKLAEMDLDVIQ
                     NEEKDPTIMIVFTNDKVEEVVIKQLGTVTAGKVIGSIKM"
     gene            7812..8645
                     /locus_tag="IXT20_002003"
     CDS             7812..8645
                     /locus_tag="IXT20_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HAQ4860439.1"
                     /translation="MKFMTLNTHSWLEPEPEKKLQELADKILLEDYEVIALQEINQLL
                     ESEEVEPAKLMKFCPVKNQVPIRKDNFAYRLVQLLKEHGKEYFWSWEMSHIGYDKYEE
                     GNALLTKKTLESQVLTVSQFQKKEDYQTRKILIGKTKIDDQDIFVSSCHFSWWTDKKS
                     GFYFEWKNLENYFLETRVPLFFLGDFNNPVDSQGYYTVRESCLLLQDSYVVANEKGKA
                     ATVEKKIDGWEQNTEKLRIDFIFVPEGMQVKKYQRIFDGIDSPIISDHYGVEIELDVN
                     E"
     gene            complement(8747..10447)
                     /locus_tag="IXT20_002004"
     CDS             complement(8747..10447)
                     /locus_tag="IXT20_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4860440.1"
                     /translation="MMNLSKVCKGVRQLKKYGPFLAIFFIALAFLMPHLFTHKLILGA
                     DAPFHYNRFYDAAQQIKYGDFHYFPSLYGFQQSGRIVNAVYGPFFAYLQGLIVLLAGS
                     WFHYQILSNFILYLISGFSMLILLRYVNVRNWVSVPVAVLFMSTYSIQYWSINQGFTS
                     WGTCFYPLCMIPIADFVLHKKFPVIKVAVSVGLMTQIHLLSSIMLALMYIPFYLYYFF
                     NQTEKGKALLQLIKSVLLYFVLTANIWAGLAIVYSGNKILPPFVNREMSQKAIDLDGS
                     YWLHYPKAFPILILSGFLLFIFFQQKNTLFQKIVLYTSLVFFFLSTSLIPWTTLIEKN
                     VPFISLIQFPFRFFVPFTLLLLLYLALSLNQWSEQKWFRLLSIFGILIFSAQTIQNLY
                     QHLEKWENETFVSRHTYLFDTPEEARKTFFSKDMSTSLFVFQKTTPDYLPIYKETKDN
                     KYDRYNEEILENETTFIKTHPGGLLTIKWKNTGDKTVHIPVIVYDRTVLQQNGKTLTD
                     YEVTDIGTPIVKQQKGINELTLHYQTPIYFYFILSLTLIGWFTLLCLFIYHRYKLLRA
                     "
     gene            10954..12114
                     /locus_tag="IXT20_002005"
     CDS             10954..12114
                     /locus_tag="IXT20_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAQ4860441.1"
                     /translation="MKQSKQKQSRRLLFRLFLLICIVVGLLIALYPFYVDSLNSFIDQ
                     KRIEQVQKRTAAEIEVQRKKMEEKNQRLTDQGFNPGTDPFNEKNRNESTTSSQLEEWL
                     IGSINIPKIQLNISLYDRLNGRILENGAGVLQGTSFPLGGNSTHSVISAHSGLPNRRL
                     FTELDRLENGDTFILTVLGEKLAYQVKNIQVVLPDDTSVLTISEGEDLVTLLTCTPYM
                     INTHRLLVTGHRIPYNESVKKEEGKGNQERTMRQWFILAGTIIAVVILLVFIGRLIYQ
                     YRLSKKVLDFSFIISDSSGKPVNGSSFILKHKKKTLTRNGVPFSVQSDHHGKVKLDQL
                     PGGTYRIVSADDPKVAVSFGIRKVKQEKIYFFEGRKLVKELQKNGFYFKINE"
     gene            12130..12573
                     /locus_tag="IXT20_002006"
     CDS             12130..12573
                     /locus_tag="IXT20_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ4860442.1"
                     /translation="MIRTTTKLSAKELESVLSIWLHANLDSHEFIPSTYWENQVESVR
                     KALPDAVIYLAEIDQEIIGFAGVIDQFIAGIFVKREYRQQRIGTQLMKKIKETNKTLT
                     LTVYQKNQSAIHFYLSQGFTIQTQQKDEAVNEEELVMKWNQNADF"
     gene            complement(13056..13586)
                     /locus_tag="IXT20_002007"
     CDS             complement(13056..13586)
                     /locus_tag="IXT20_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenolic acid decarboxylase"
                     /protein_id="HAQ4860443.1"
                     /translation="MKEFKELEDFIGTHFIYTYDNGWEYEWYAKNDHTVDYRIHGGMV
                     KGRWVKDQEADIVKLTDGVFKITWTEPTGTDVALDFMPNEKKLHGMIFFPKWVEEHPE
                     ITVTFQNEHIAEMEAAREKYETYPKLLVPEFATITYMGDAGIDNEDVISEEPYDGMPD
                     DIRSGKYFDENYKRVK"
     gene            13691..14242
                     /locus_tag="IXT20_002008"
     CDS             13691..14242
                     /locus_tag="IXT20_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAQ4860444.1"
                     /translation="MARKNTLRYILLGLLSKKKMTGYELNQSFKNEIGEFWQAKHSQI
                     YPELAKMEEQGIIQHQVEITGEKLEKKVYELTEEGKEQLAEWIHTPTNELPVNRDEFV
                     LKLYFVKDVNDPALVEIIQQQRDLHEEKRLHLLERKKTIFPTEKEKHENYGHYLILAH
                     AINRETEYANWLTDVLEESNRKK"
     gene            complement(14395..14868)
                     /locus_tag="IXT20_002009"
     CDS             complement(14395..14868)
                     /locus_tag="IXT20_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAQ4860445.1"
                     /translation="MVGASLKRLLENDQLVVGISELSDIVGVSPRQLRYWEQKGFIRS
                     IEADANCPRKYRLPTVIKVELIKKYLDDGFTLSKAAEKAEIRQKKMHHVRKVFSKAIK
                     DIELIDERYTILQIGNFKEENAKMYIIHDNETDELTYKLLPVDEKADYEQLLKEL"
     gene            15006..16478
                     /locus_tag="IXT20_002010"
     CDS             15006..16478
                     /locus_tag="IXT20_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HAQ4860446.1"
                     /translation="MSNNQPVDIYGKPYNRTLLVVVLLIGTFCTVLNQTLLTTAFPTL
                     MKAFDISASDVQWLTTGFLLVNGIMIPITAWLINKFSSRNLYLSAMTIFLIGTITCFT
                     APNFSTLLIGRLIQACGVGVSMPLLQNIMLSIFPPEKRGSAMGMSGIVIGLAPALGPT
                     LSGYIIDNYHWRDLFGMVLPIVVLVLVLAFFLMKSVIPLSNPSIDILSAILSTIGFGS
                     LLYGFSSVGDDGWGSLKVIGFLVVGVIFIALFSWRQLHLEHPFLELRVFKSSTFTIAA
                     VLAGVTNMAMVGAEMVVPLYIQNIRGESAFHSGLMLLPGALIMGAMMPVTGAIFDKYG
                     AKRLAITGMFILTAATAPFAFLTKTTPVLYIVILYAIRMFGISMVMMPVTTSGMNALP
                     NNLISHGTAVNNTFRQIASSIGTAILISVLTNVTKANIPSKSVLDNTPLAYKDQAINA
                     TLSGYHAAFIVAIIFGILGFIIAFLLKKKEPVLMKAGDNA"
     gene            16475..17185
                     /locus_tag="IXT20_002011"
     CDS             16475..17185
                     /locus_tag="IXT20_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4811 domain-containing protein"
                     /protein_id="HAQ4860447.1"
                     /translation="MIIGVLIVSLLAFALTNVFAKKTWQTFLSLIFGLIFVASLSLIV
                     ANLSNHFGMEKVTETKTEKIVSSADSQGADMLLYKALGNGKEKVYLYRTNEKQEKPKA
                     TGTDNETNKVEKTDGDAEKVTKTTYWEYKNDMYKFWFNIADNNHEYDSRVNTFKIPET
                     WVELSTDQAAKLAELVKKQQSTMESEAKAYVQDGMVKAMTENPKMSKAEQEQRTKALA
                     AEFQQQAFAKLVKEAKGE"
BASE COUNT         5396 a         3119 c         3569 g         5267 t
ORIGIN      
        1 agataaacaa tgtgttagaa gtagtatctt cttttacata aaaagctgac cataggataa
       61 atggtcagcg tatttagtga gtcagctatt tagtatgggt tttctaaagg aacgactgtc
      121 tctcttttga tcaagcgatg agggatgacc aaacgtacgc cactgctcaa ttcttcatgg
      181 atcaattcgc gcagcttttg catagcgaca cgtcctaatt cggagatatc gatatcgata
      241 cttgtaaggt aagggtgtgt taatgttgag aagatcgagt tgttgaagct aatgacggag
      301 agtgtctctg gaatgacata gccatacatt tgcgccaact gcatcgtgcg aagggcgaag
      361 atatcgtcaa tgaccaccaa tgctgtggct tcgttttctt tcaacgtttt ctcaaaggac
      421 agataatcct ccggatgctc tagtactaca ctggataacg caggaagacc agccatcatc
      481 attgcttttt gataaccgaa atatcgttcg aaatacaaac tttcatgagt cgtgtttgtc
      541 acaaacagga tattcttatg tccattctgg atcaaaaatt ctgttgcttg tttacctaga
      601 agctggttat cattatctac ataaacgatt tcgttctcat gatgataagg ctgtccgatc
      661 aaagtgaaag ggatgtggtt ttggtataaa taatcaatga cagtatcttg actatctgaa
      721 tacactagaa taaaaccatc tacttgtttt tgtaaatgca tacgctggac gttctctaac
      781 agcaattcaa aatttttagc agtagcgatt gctgtagtca tatcataatt ccgcgcttct
      841 tcgttgattg cttccatgat ctcaagataa aatgggttac cgatccgctc ttttgaatca
      901 agagggggta agattacgcc gatggcactg gaaatgcgtt ttcctagatt gcgggcagtc
      961 atgttaggaa cataacctag ttcatccata atttttcgga ctttcttctt tgttgtctcg
     1021 gaaatactag gatgatcgtt gatgacacga gagacggtcg aggtggcaac accggctttt
     1081 ttcgccacat cttttacggt aatagccatg ataaaaacac ctttctcttg ttattgtgca
     1141 gagaaccgat aaatcgtctg actgatccgt tcttgaccag gatggaggtt gatcgatcca
     1201 aatttgggga aatgaacgat atcagggatt tcttgcggtt cgattgctaa tccgtagttt
     1261 gaatgcatct gttttccatt gacagagaaa tcagcttcga atccagtcgt agaaaataat
     1321 accatacttt tattagaagt tgctatagtc atttggcgac cagaatgctt ctcgaataat
     1381 tgtaaggaag ttttggaaag cctaggatga tgcaaaagaa atacatcatc tagtccatct
     1441 ggataacatg caaggatctc ttgcagtgtt ggggattttc taaagtcgat tggcagtttt
     1501 tctttttttg tccaatttcc agtaggcagt ttttcttgat ctaattcaag cacaccatct
     1561 aaaaagaccg ttaactgatg ttcatagatg tctcttgttc catttcctga aaggttgaag
     1621 taggcatggt tggtcggatt gcagatggtt tcaacaaggc tactagcaga ggttgtcatc
     1681 tctaattcgt tttttgttaa acggtaagtg atcgtagcag tgattggtcc tggataggta
     1741 gaaaattcat cttttaagta aaagaccaca cctattgatg atggagtttt gaaaacttct
     1801 acttcccaaa attggaaaga ccagcctttc gtgccaccgt ggatatggtg tgatccgcag
     1861 tttttctcca atctatgctc accccaagaa gcatcccgga tccttccggc tacaggtccg
     1921 acagttgctc caaaaaagct tttgtctgtt agaacatctt cgaaacgatc gtaagacaat
     1981 aaaacatttt ctgattttcc ttctttgtct ggtgcgaaga tttggtgcag gcgggcaccg
     2041 taattgagaa gaacgacttc taagtagtcg ttcttcaatg tgattttctt tatttcagtc
     2101 tctggaactg gtacgacgga gatctctgga ttatccatgg tcatgccact tttctttcaa
     2161 gtaatcgatc gtaagaaaag tcgtatcagg gacaagcggg atctcctctc caaggtcttc
     2221 agcacgtccc acgccaattg gttgagcgcc acttgagagg atggcttgga taccggcttg
     2281 cgcgtcttcg atcccgatac attcttctgc ttttagtccg acagcttttg ctgcaaggag
     2341 gaagatatct ggcgcaggtt ttccgtttgt tgcttctgcc gggtcggcaa tagcatcaaa
     2401 gtaaggtgta agacccattt tttcaagtag gaatggcccg tttttacttg cagaagcaag
     2461 agcaatcttg attttttctt cttttaaaga atccagtaaa ggcacgaccc cggggaagac
     2521 atcttttggt tcgatcgtct ggatcatctc taaataataa tcgttttttc ttttcgctaa
     2581 ttctgcaaat tcttcctttg aaaaagagtg ttcttttccg ccataagcta aaagcaaaga
     2641 gagtgagtct tctcggctga cgccttttag ctgttcgttg aatgtgtggt cgatcgtaat
     2701 gccgatttcc tgaccaagct ttcgccaagc acgataatgg aattcagcag tatcggtaat
     2761 gacaccgtct aaatcaaaca ataccccttt aaacatttgc ttcctccttt cttagcgtta
     2821 tttctagacg atccgctaaa gaataagact gttcatagac gtaaatttct agaggagaac
     2881 cttctaataa ctcgaaagta acttgttcat ctgtgactga gatgaacaat aaacgtccgc
     2941 gataattgat atggaaagaa taagctttcc aagtttttgg cagaaatggt gcgaatctca
     3001 actgatcatg ggcagttttc atttgagcaa atccttggac aatcgacaac cagctaccag
     3061 tcatggaagt gatgtgcaag ccgtcttctg tgtcgttatt gtaattatcc aaatccaagc
     3121 gagctgttcg ctggtacatt tctacagctt tgctttccat tcctaattct gctgctaaaa
     3181 tcgaatggat acttggcgaa agggaagact catggacagt cattggttca tagaaatcaa
     3241 agtttttttg cttttcttct tttgtaaaag tatcattgaa gaaataaatc ccctgtagta
     3301 catctgcctg cttgatgaag caagatctaa ggattttgtc ccaagaccaa tgctgattca
     3361 gaggaagatc gcttgtggga atatcggata caggcatgag atctttatct aagaatgtgt
     3421 catgctggac aaagattcct aattcttcgt caactgggaa gtacatgttc tctgcgatat
     3481 ctttccattt ggctagttct tcttcgctga tagtgatagc agcttctttt tgataagtta
     3541 aataattttc tcgcgtgtaa cgcaagaccc aagctgcgat cgtatttgta taccaattgt
     3601 tattgatatt gttctcatat tcgtttgggc cggtcacacc gtggatcatg tatttatttg
     3661 ctcgttttga aaaatggaca cggtctgccc agaagcgtgc gatttctgct aaaacttcca
     3721 gtccttcatt tttcaaatat tccgtatcgc cggtgtaatt cgtataattg tagattgcat
     3781 aggcaatcgc gccatttcgg tgaatctcct cgaatgtgat ttcccactcg ttatggcatt
     3841 cgacaccagt gaaggtgacc ataggataaa gcgcgccttt taatccttgt tgctgagcgt
     3901 tgtggattgc ttgcggcagt tgattatgcc gatatttcag cagattctta gttacttcag
     3961 gtttggctaa agcaagataa agagggacag catacgcttc tgtatcccag taagtggctc
     4021 cgccgtattt ttctcctgtg aatcctttgg ggccgatatt caatcgttca tcttcgccgt
     4081 agtaagtaga gaatagttgg aacaaattga atcgaatacc ttgttgtgct tcttcgtctc
     4141 cttcgatgac cacatcggca agcttccatc gttctgccca agccgctgtt tgctcttctt
     4201 ttgcttggat ataattgttt gtctggttcg ttaattctga gacagctttt tctgcttgct
     4261 gcgcttctgg aatatctcta ctagtaagaa cgataatttc tttttccact gaccatgtct
     4321 caccttgaga taattctgca gacaagcaac catgtaatgc taaagtatct tcgtgatagt
     4381 cagggatgac tgattttcca ttaacgaaat ggcgcattgc tgcagtaaca gtaaattgtt
     4441 caatcccaaa gttgtttgga atagttttag tcgtcaaata gccgatttct tctatagttc
     4501 cacgatcaat ctcattccaa aaatgttcat catagttgct gtcttcgttt tgaacatttc
     4561 catccaattt tgatacgagt ttgactgttc cgctaccttc aagcatttct atagttaaat
     4621 agatataagc tgattctttt ttgacaatgc tcagaaaacg ttcgaaggaa atgcgtactt
     4681 tattagtaga agtagtgatt gtgaatgtac gagataatac accatttttc atatctaatt
     4741 cccagtaaaa gtcttctggt tccaaagaag ccaagtcgat cagctgatcg ttgatcgaaa
     4801 tatctatcgc gataaagttc aaggcgttga tcacttttcc aaagtattct ggataaccat
     4861 ttttccacca gccgacacgt gtcttatcgg gataccagac acccgccaaa taagtacctt
     4921 gatggtggtc ccctgagtaa gtttcttcaa agttcccgcg catccccata taaccgtttc
     4981 caatacttgt taaagattct tgcagtcgaa gatttttttt atctaattgt gtcgtgctga
     5041 ttttccatgg atcaatagca aatagtcgtt tgatctgttt catttttctc ctcctaatga
     5101 gctgttttat acgttcataa taaacgcaac cgattgcaca agtcaataga caaaagaaaa
     5161 tatttcatag aaaaaagctt aaaaaaataa atttttagga gaaaattata gaagggaaca
     5221 cacgtattat aggttttata gaatggtata gacagaaaaa taaaaaataa agcatttaca
     5281 aaaaagaaaa cgcacgctat aatacgttta acgaaaccga ttgcgtaaag gagctaaacg
     5341 gaatgaaaaa gatatttagt tttgaatttt ggcaaaaatt tggaaaggct ttaatggttg
     5401 tggtagcagt tatgccggca gctgggttga tgatcagtat agggaaatca attcccttga
     5461 tcaatcctga ttggacgccg cttgtcacaa cgggcggagt aattgaaaat attggttggg
     5521 caattattgg taatcttcat attttatttg ctttagcaat aggaggaagt tgggcaaaag
     5581 aaagagcagg cggagcattt gcagctggga tctcatttat tttaatcaat cggatcactg
     5641 gggctatctt tggtgtcaca agtgacatgc ttgcgaatga agatgcattt acccacactc
     5701 tgttcggtac gaagatcatg gttaaaggat tttttacaag tgtattggaa gcaccagctt
     5761 tgaatatggg agtattcgta gggatcatcg ctggatttgt cggggcaatg gcttataata
     5821 aatactataa ttatagaaaa ttgccggatg ctttgtcatt tttcaatgga aagcgctttg
     5881 ttccgtttgt tgttatctta tggtcaacaa tcgtggcatt gattcttgct gtggtgtggc
     5941 caaacgtaca ggctggaatc aataattttg gtttatggat tgcagaatca caagaaagcg
     6001 caccgattct agcaccgttt ttatttggaa cattagaacg tttactattg ccatttggat
     6061 tgcatcatat gctgacgatt cctatcaact atacacagct tggcggatcg tatgagattt
     6121 tatcaggcgc acaggcgggg acacaagtat ttgggcaaga tccgttatgg ttggcttggg
     6181 ccactgattt agtgaatctt aaaggtgcag gggatatgtc gcaatatgag tttgtgttgc
     6241 ataactggac gccggctcgt ttcaaagtag gacaaatgat tggttcttca ggtatcttga
     6301 tgggaatggc ttttgcgatg tatcgtaatg tcgatcctga taagaaagca agatataaat
     6361 caatgtattt ttcagcagcg ttggctgtct ttctaacagg tgtaacagaa ccattggagt
     6421 tcatgtttat gtttgctgct gtgccattat atgtcattta ttcagtgatc caaggggctg
     6481 catttgcgat ggcagatatc ttgccattgc gggtccattc atttgggaat atcgaattgc
     6541 tgactcggac accgttagct atcaaagcgg gtctaggtgg agatcttatc aacttcgtgt
     6601 tgtgtgtgat cgcttttggt gttgttacgt atttcttagc aaatttcttg atcaaaaaat
     6661 tcaactttgc tacaccggga cgtaacggaa attacgacaa tgacagtgaa gaaaccgttg
     6721 tatctaacag cggaacagga acagcagatc agcaagtggt ccagatcatt catttgctgg
     6781 gaggaaaaga aaatatcaaa gatgtcgatg cttgtatgac tcggttacgc gtaagtgtaa
     6841 atgatcgaga aaaagtaggg actgaagaag cctggaaacg agcaggggcg atgggattga
     6901 tcgtcaaaga caatggagtc caagcagttt atggaccaaa agcagatgtc ttgaaatcag
     6961 atatcgaaga tctgctgcaa tcaggagcag agattccaga gccagaaatg gtttctacag
     7021 gaaatggaca agcaggtagt aagcaatact taggtataga acaagaacta gtttccgcag
     7081 cttctggaga agtgattcct ttatctgaag taaaagatcc ggtattctca caaaaaatga
     7141 tgggtgacgg ctttgctgtt attccaacat caagagaagt tgttgcgccg atcgctggaa
     7201 aagtgacaag tattttccct agcaaacatg caattgggct ggaaacgaaa gatggtatcg
     7261 aagttttgat ccatatggga atcgatacag tccaaatgaa tcagccagct tttgagattg
     7321 ctgtcaaaga aggccaagaa gtaggaacag gaacgaaatt agctgagatg gatcttgatg
     7381 tgatccaaaa cgaagaaaaa gatccaacga tcatgatcgt atttacaaac gataaagtag
     7441 aagaagtagt gatcaagcaa ttaggtacag tgacagctgg taaagtgatc ggctccatta
     7501 aaatgtaagg agaggttgtg aaaaagctgt cctgcatcaa gtcataagaa cagccaaaca
     7561 aaaatagctc ctcctatttt tcgtttggtt gggcttatta ccgagaagca gcagtttgaa
     7621 tactgtttag tggagaggtc gtgaaaaagc tgccctgcac caagtgataa aaacagccaa
     7681 acaaaaaata gctcctggtc cattgagctt attactgagg agttagcagg agaacactat
     7741 ttgctggaga ggttgtgaca aaaattttgt cacaaccctt ttgtttcgaa atgaagacaa
     7801 ggagaaaacc aatgaaattc atgacactca acacgcatag ctggcttgag ccagaaccag
     7861 agaaaaaatt gcaagaatta gcagataaga tcctgttaga agattatgaa gtgattgctt
     7921 tacaagaaat caaccaatta ctggaatctg aagaagtaga acctgcgaag ctaatgaagt
     7981 tctgtccggt aaagaatcaa gtacctatcc gtaaagataa ttttgcctac cgtttagttc
     8041 agcttttaaa ggaacacgga aaagagtatt tttggagttg ggaaatgagc cacatcggtt
     8101 atgataaata tgaagaagga aatgcactgc ttacgaaaaa aacattggaa agtcaggtcc
     8161 ttaccgtctc ccaatttcaa aagaaagaag attatcagac acgcaagatc ttgattggga
     8221 aaacaaagat agatgatcag gacatttttg tctcgagctg ccacttttct tggtggacag
     8281 ataaaaaaag tggcttttat tttgagtgga aaaacttgga aaactacttt ttggaaactc
     8341 gcgtaccact ttttttccta ggtgatttca ataatccagt agactctcaa ggttattata
     8401 ctgtaagaga aagttgcctt cttcttcaag attcgtatgt tgtcgcgaat gaaaaaggaa
     8461 aagcagctac tgtggaaaag aaaatagatg gttgggaaca aaatacagaa aaattgcgta
     8521 tagattttat tttcgtccct gaaggaatgc aggttaaaaa atatcaacgg atttttgatg
     8581 ggatcgatag tccaatcatc agtgatcact acggggtgga aatcgaatta gatgtgaacg
     8641 aataaaaaat ataacatata acataaaaaa actgattaaa ataaaactct ccaaacgatt
     8701 ataccgtttg gagagttttt tgatcggatg tatttatccc ttttgcctaa gcacgtaaaa
     8761 gcttgtatct atgataaata aataaacata gcaaggtaaa ccagccaatc aaagtaagag
     8821 aaagaatgaa atagaaatag attggcgttt gataatgaag cgtgagctcg ttgattcctt
     8881 tttgctgctt cacgattggg gtcccgatat ctgttacttc ataatcagtc aatgtttttc
     8941 cattttgctg tagcacagtc cgatcgtaga cgataacagg gatatgaaca gttttgtccc
     9001 cagtgttttt ccacttgatt gtcaaaagac cgcctggatg tgtttttata aaagttgttt
     9061 cgttttctag tatttcttcg ttataccggt cgtatttatt gtcttttgtc tccttataga
     9121 ttggcagata gtcaggtgtg gttttttgga aaacgaacaa gctggtagac atatctttgc
     9181 tgaagaatgt tttccttgct tcttccggcg tatcgaatag ataagtatgt cgactgacaa
     9241 atgtttcatt ttcccacttt tccagatgct ggtataagtt ctggatcgtc tgtgctgaaa
     9301 aaataagaat gccaaatata cttaaaagac ggaaccactt ttgttcagac cactggttca
     9361 gtgaaagggc aaggtaaagt agaagcaata gcgtaaatgg cacgaaaaat cgaaatggaa
     9421 actggatcaa actaataaaa ggcacatttt tttcgatcaa tgtcgtccat ggaatcaagc
     9481 tagtagaaag aaaaaagaaa acaagacttg tgtacaacac gattttttgg aataaggtgt
     9541 tcttttgttg aaagaaaata aaaagcaaaa aacctgataa tatcaatata ggaaaggcct
     9601 tagggtaatg gagccaataa ctgccatcca gatctatcgc cttttgactc atttctctgt
     9661 taacaaaggg aggaagaatc ttattcccac tgtaaacaat agctaatcct gcccaaatat
     9721 ttgctgtcag aacaaaataa agcaagaccg acttaatcag ttgaagcagt gcttttcctt
     9781 tttcagtctg gttaaaaaag taataaagat aaaaagggat atacatcaaa gctaacatga
     9841 tcgaagacag taaatggatc tgtgtcataa gtcccactga gacagcgact ttgataacag
     9901 ggaacttttt atgaaggacg aaatcagcaa tagggatcat acataaagga taaaaacaag
     9961 tcccccagct agtgaatcct tggttgatag accagtattg aatagaatac gtactcatga
    10021 ataaaacggc tactggtaca cttacccaat ttcgaacatt gacatatctc agtaaaatca
    10081 gcatagaaaa tcctgaaata agataaagaa tgaaatttga aagaatctga taatgaaacc
    10141 agctacctgc aagcaacacg atcaatcctt gtaaataagc gaaaaatggg ccgtaaacgg
    10201 cattcacgat cctgccactt tgctggaatc cataaaggga aggaaaataa tggaaatccc
    10261 catatttaat ttgttgcgcg gcatcataga accgattgta atggaatggg gcatcagcac
    10321 ccaaaattag tttatgtgtg aataaatgag gcatgagaaa tgccaaagca atgaaaaaaa
    10381 ttgctaaaaa tgggccatat tttttcaatt gtcgaactcc tttgcaaact ttagataaat
    10441 tcatcatata tcctatgcga gaggtaggca agaaattaga aaattcatga aaatatttat
    10501 gaattttaca agaaaaacat aagtgaaagt tctaaggaga gaggatgcga cataagtttt
    10561 gttgcatctc cttgttttga aacagaattt ttcttaatac tcgaaacaaa gtagcttttt
    10621 catgacctct cggtaaacgg tgttcaatca gctgcttctc ggtaataagc tcactagcct
    10681 agaaaatatg agaagctatt ttctgactag cgctcttatt actcaaagca taacgcagtt
    10741 ttcacaacct cttccttaaa cggtgttcaa aagcttgcat ctgcggtaat aagcccaacc
    10801 caacaaaaaa tatgagtagc tatttttttg ggctgtactt attacttgat gcaggacagc
    10861 tttttcacaa cctcatcggc tttcattttc tatttttttc ctgaacccat ataataaaag
    10921 aaagaacaaa aaaacttgca agggaggaca catgtgaagc agagtaagca aaaacagtct
    10981 cgtcgacttt tgtttcgttt gtttttatta atatgtatcg tcgtagggtt actgatcgct
    11041 ttatatccat tttatgtgga ttctttaaat agttttattg atcaaaaacg tatcgaacaa
    11101 gtacaaaaac ggaccgcagc tgaaattgaa gtgcagcgaa agaaaatgga ggagaaaaac
    11161 caacgattaa ctgaccaagg attcaatcct ggtactgatc ctttcaatga aaagaacaga
    11221 aatgaaagta caacatccag ccaattagag gaatggctga tcggaagtat caatatacct
    11281 aagatccaat taaatatttc actttatgat cggctcaatg gtaggatcct tgaaaatgga
    11341 gcaggcgtct tgcaaggaac ctctttcccg cttggtggaa attcgacgca ttcagtgatc
    11401 tctgctcaca gcggtttacc taatcgacgg ttatttaccg aattagatcg tttagaaaac
    11461 ggagacacgt ttattttgac tgttttgggg gagaaattgg cttatcaagt taaaaatata
    11521 caagtggtac tgccagatga cacgtctgtt ctaacgattt cagaaggaga agacttagtt
    11581 accttgctta catgtacgcc gtatatgatc aacacgcatc gtttgctagt gacaggacat
    11641 cggattcctt ataacgaatc ggtaaaaaaa gaagaaggaa aaggaaatca ggaaagaacc
    11701 atgcggcaat ggtttattct tgcaggaacg ataatagctg tagtcatact gttagtgttt
    11761 atcggacgac taatctatca gtatagatta agtaaaaaag tgcttgattt ttcatttatt
    11821 attagtgatt cgtcagggaa accggtaaat ggtagttcat tcattttgaa acataagaaa
    11881 aaaacactta ctagaaatgg tgtgccgttt tcagtacaaa gcgaccatca tgggaaagtc
    11941 aagctggatc aattgcctgg tggaacttat cgaatcgttt cagctgatga tcctaaagtt
    12001 gctgtttctt ttggtattag aaaagtaaaa caagagaaga tatacttttt tgaggggcga
    12061 aaacttgtaa aagagcttca aaaaaatggt ttttatttta aaatcaatga ataagtaagg
    12121 agagaaaaaa tgatccgcac aacaacaaaa ttgtctgcca aagaactgga atctgttcta
    12181 tcgatttggc tgcatgctaa cttggattct catgaattca ttccatcaac ttattgggag
    12241 aatcaagtag aatctgttag gaaagcttta ccagatgcag tgatttactt agcagaaata
    12301 gatcaagaaa ttattggttt tgctggggtg attgatcagt ttatcgcagg aatctttgtg
    12361 aaaagggaat accggcaaca aagaatcggt acgcaactaa tgaaaaaaat aaaagaaacc
    12421 aataaaacac tgactttgac agtctatcaa aaaaatcaat cagctattca tttctatctt
    12481 tcacaaggtt ttacaattca aacacagcaa aaagatgaag cagtaaatga ggaggaactt
    12541 gtgatgaaat ggaatcaaaa cgctgatttt tgatgaacag aataacgaaa taatgctccc
    12601 agcctaattt tttgctggga gcattatttc attttgaagt cactaattta actgtgtgaa
    12661 tatgcagttg ttacaatctt tgataaacgg tgttcaatca gctgaccctc ggtattagct
    12721 caaaaaacgg caaaatatga ggaactattt ttccatttgt ttctctaata ctcaggtcaa
    12781 aacgctgatt tcacaacctc ttgataaacg gtgttcaaac ggctgcttct cggtaataag
    12841 cccactagcc tagaaaatat gaaaagctat tttctaacta gcgttcttat tactcaaagc
    12901 ataacgctga tttcacaacc tcttgataaa cggtgttcaa tcagctgacc ctcggtatta
    12961 gctcaaaaaa cggcaaaaca tgaggaacta tttttccgtt tctttctcta atactcaggt
    13021 cagaacgctg atttcacaac ctctctttca caaccttatt ttactcgttt ataattttca
    13081 tcgaagtatt ttccgctacg gatatcatct ggcattccat cgtatggttc ttcgctaata
    13141 acatcttcat tgtctatacc tgcatcaccc atataagtga tcgtagcaaa ttcaggaaca
    13201 agtaattttg gataagtttc atatttttca cgagctgctt ccatttctgc gatatgttcg
    13261 ttttggaaag taacagtgat ttctggatgt tcttcaaccc atttagggaa aaagatcatt
    13321 ccgtgaagtt tcttttcatt tggcataaag tccaatgcca cgtctgtccc tgttggctct
    13381 gtccaagtga ttttgaatac gccgtccgtc aatttaacga tatcagcttc ttggtctttt
    13441 acccaacggc ctttgaccat tccgccatgg atacggtaat caacagtatg gtcatttttg
    13501 gcataccatt catattccca accattgtca tacgtataga taaagtgagt tccaataaaa
    13561 tcttccaatt ctttaaattc tttcatgttt aagcatctcc ttataaatta aaacatgtaa
    13621 taaactacat gttttcgatt actcatttat gatagaatgg atttaaagat ttgtcaagga
    13681 gggaaaactg atggcaagaa aaaatacact tcgatatatt ttgttagggt tattaagtaa
    13741 gaagaagatg acaggatatg aattgaatca atcgttcaaa aatgaaatag gagaattttg
    13801 gcaagcaaaa catagtcaga tttatcctga acttgcaaaa atggaagagc aaggaatcat
    13861 tcagcatcaa gtagagatca caggtgaaaa attggaaaag aaagtttatg aattaacaga
    13921 agaagggaag gaacaattgg cagagtggat tcatacaccc accaatgaac taccggttaa
    13981 tcgagacgaa tttgttctga aattgtattt cgtcaaagac gtgaatgatc cggcattagt
    14041 agaaattatt cagcagcagc gtgaccttca tgaagaaaaa cgacttcatc tattagagcg
    14101 aaaaaaaacg atctttccta ctgaaaaaga aaaacatgaa aattacggac actacctgat
    14161 tttagcgcat gcgatcaatc gggaaacaga atatgcaaat tggctaacag atgtattaga
    14221 agaaagtaac cgaaaaaaat aaaaaaatat ccgaacagtg atggagaatt cctttctata
    14281 tgaaagaaac gcagatcacc gttcggatat tcttatgttc aaacaggcct tttttcaagt
    14341 atttatgtag catctactta ttaaaaagct tggtacttag gagattagtg gcgtttagag
    14401 ttcttttaac aactgttcgt agtcagcttt ttcatcgaca ggaaggagct tgtaagttaa
    14461 ttcgtctgtc tcgttgtcat gaatgatgta catcttagca ttctcttctt tgaagttacc
    14521 gatttgtaaa atcgtatagc gttcgtcgat caattcgata tctttgattg cttttgaaaa
    14581 aactttacgg acatggtgca ttttcttttg gcggatttct gccttttctg cagcttttga
    14641 caaagtaaat ccgtcatcta aatatttttt gatcagttct acttttatga ctgtaggtaa
    14701 acgatatttt ctaggacaat tcgcatcagc ttcgatcgaa cgaatgaatc ccttttgttc
    14761 ccaatacctt aattgacgtg gagacacacc tacgatatca cttaattcac tgatgccaac
    14821 aaccaattga tcattttcca acaaacgttt aagggaagcc ccaaccattt ttgtcactcc
    14881 ttttgtcctc ttgcttccct taattatatc cgtattaact tatgtgtcaa gtaggttgac
    14941 aaatagcgaa tttaggacta aagtattcta gtagtttcag aaaaaattca gaaagaagga
    15001 gaaatatgag taacaaccaa cccgtagata tttacggaaa accatataat cggacacttt
    15061 tagttgttgt cttattaatt ggtacatttt gtaccgtttt gaatcagacc ttattgacga
    15121 cagcttttcc tacattgatg aaagctttcg atatttcagc gtctgatgtg caatggttaa
    15181 cgactggttt cttgctagtc aacgggatca tgatcccaat aacagcatgg ctgatcaata
    15241 aattcagttc aagaaattta tatctttctg ccatgacgat ctttttgatc ggaacgatta
    15301 cttgctttac agcacctaat tttagtacat tactaatcgg tcgtttgatc caagcttgcg
    15361 gtgtgggagt atcgatgcca ttattgcaaa atatcatgct atctattttc ccgccagaaa
    15421 aaagaggatc agcaatgggg atgtcaggga tcgttattgg acttgctcct gctttaggcc
    15481 ctacattatc tggttatatc attgataact accattggcg tgacttgttt ggtatggtac
    15541 tgccaatcgt tgttcttgtc ttggttttag cattcttcct aatgaaaagt gttattccat
    15601 tgtcaaatcc tagcattgat attttatccg ctattttatc aacgattgga ttcggtagtt
    15661 tactttacgg attttctagt gtaggagatg acggctgggg aagcttgaaa gtaattggtt
    15721 tcttagttgt tggtgtgatt ttcatcgcct tgttctcatg gcgtcaatta catttggaac
    15781 atccattctt ggaacttcgt gtattcaaat catctacatt cacgattgca gctgttttag
    15841 ctggtgtaac aaatatggcg atggtagggg cagaaatggt cgtaccattg tatattcaaa
    15901 atattcgtgg agaatcagca ttccattctg gcttgatgtt gttgccgggt gcattgatca
    15961 tgggggctat gatgcctgtg acgggtgcga ttttcgataa atacggcgcg aaacgtttag
    16021 ccatcacagg gatgttcatt ttgacagcgg caactgcacc atttgctttc ttgacaaaaa
    16081 cgactccagt tttatatatc gtgatccttt atgcgatccg tatgtttggg atctccatgg
    16141 tcatgatgcc ggtaacgact tctggtatga atgcattgcc aaataacttg atcagtcatg
    16201 ggacagcggt aaataataca ttccgtcaaa tcgcaagctc gattggtaca gctattttga
    16261 tcagtgtgtt gacgaatgtg acaaaagcaa atatcccaag caaatctgta ttggataata
    16321 cgccattagc ttataaagat caggcaatca atgccacact gtcaggttac catgccgcgt
    16381 ttatcgtagc gatcatcttt gggattcttg gctttattat tgccttttta ttgaagaaaa
    16441 aagaacctgt tctaatgaaa gcgggggata atgcatgatt attggagttt tgattgtcag
    16501 cttgcttgct tttgctttaa cgaacgtgtt cgctaaaaaa acatggcaaa cctttttatc
    16561 tttgattttc ggactaatct tcgtcgcttc actaagtttg atcgtcgcta atttgtccaa
    16621 tcactttgga atggaaaaag taacggaaac aaaaacagaa aaaattgttt ccagtgctga
    16681 ttcccaagga gcagatatgc ttttgtataa agctttaggg aatggaaaag aaaaagtgta
    16741 tctttaccga acaaatgaaa aacaagaaaa accaaaagca actggtacgg ataacgaaac
    16801 aaataaagta gaaaagacag acggcgatgc tgaaaaagtg acgaaaacta cttattggga
    16861 atacaaaaac gacatgtata aattctggtt caatattgct gataacaatc acgaatatga
    16921 cagccgagtg aatacattca aaatcccaga gacatgggta gaattaagta cggaccaagc
    16981 agccaagtta gctgaattag tgaagaaaca gcaaagtacg atggaaagtg aagcaaaagc
    17041 ttatgttcaa gacggtatgg taaaagcaat gactgaaaat ccaaaaatga gcaaagcaga
    17101 gcaagaacaa cgtacgaaag ccttagctgc tgaattccaa caacaagcat tcgcaaaatt
    17161 agtgaaagaa gcaaaaggcg aataatattt tgaacaccat gtaggaggtt gtgaaaaagc
    17221 tgctctgcat caagcagtaa gaacaatcag ataaaaatag cactccatat ttttcatctg
    17281 attgggctta gtgccgtaga tgcaagcttt tgaacaccgt ttgttcggaa aaagggactg
    17341 tgacacggtt t
//
LOCUS       DACGLE010000058        17216 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0534
            SAMEA3304449-rid9560453.denovo.062, whole genome shotgun sequence.
ACCESSION   DACGLE010000058 DACGLE010000000
VERSION     DACGLE010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304449
            Sequence Read Archive: ERR985064
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17216)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17216)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702225.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702225.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 20:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,045
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 27 of 67
            Pseudo Genes (incomplete)         :: 25 of 67
            Pseudo Genes (internal stop)      :: 19 of 67
            Pseudo Genes (multiple problems)  :: 4 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17216
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304449-rid9560453.denovo.062"
                     /strain="VREN0534"
                     /db_xref="taxon:1352"
     gene            9..851
                     /locus_tag="IXT20_002012"
     CDS             9..851
                     /locus_tag="IXT20_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HAQ4860448.1"
                     /translation="MLQNFINGLYDFHFLQNALITSVVIGIVSGTVGCFIILRGMSLM
                     GDAISHAVLPGVAISYILGINFFIGAIVFGLLASIIITFIKSNSVIKGDTAIGITFSS
                     FLALGVILIGVANSSTDLFHILFGNILAVQDIDKWITIGVSVLVLVTVVLFFKELLIT
                     SFDPLMAKAMGMNVSFYHYLLMILLTLVAVTAMQSVGTILIVAMLITPAATAYLYANS
                     LKTMIFISATVGACSSLLGLFIGYSFNIAAGSSIVLTAAFLFVISFLISPKQKFLRKK
                     ERKL"
     gene            848..1798
                     /locus_tag="IXT20_002013"
     CDS             848..1798
                     /locus_tag="IXT20_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HAQ4860449.1"
                     /translation="MMRKWKAVLGSLGILIALFIFGACSTNSKDKDTVASNEKLKVVV
                     TNSILADITENIAKDKIDLHSIVPIGKDPHEYEPLPEDVQKTSKADLIFYNGVNLETG
                     GNAWFTKLVKNANKEENKDYFAASDGIDVIYLEGQSEKGKEDPHAWLNLENGIIYAKN
                     IEKQLAEKDPDNKKFYKENLDKYIEKLDSLDKEAKSKFASIPNDKKMIVTSEGCFKYF
                     SKAYNVPSAYIWEINTEEEGTPDQIKHLVEKLRTTKVPSLFVESSVDDRPMKTVSKDT
                     NIPIYSTIFTDSIAEKGQDGDSYYAMMKWNLDKIAEGLSK"
     gene            1813..2046
                     /locus_tag="IXT20_002014"
     CDS             1813..2046
                     /locus_tag="IXT20_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-dependent repressor"
                     /protein_id="HAQ4860450.1"
                     /translation="MVQIKTTTILIYLLAIQELSKRKKKLKNNDLAKVLSVSPASVSE
                     MLTKLRYEDYLDIGFQLTDKGKKFIDDYKKVFK"
     gene            2377..2961
                     /locus_tag="IXT20_002015"
     CDS             2377..2961
                     /locus_tag="IXT20_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAQ4860451.1"
                     /translation="MESGLSHFEKEGFQQTKIRQLCLTAGVTTGAFYKHFTSKEALFE
                     EIIQPHINALINMYNEEFTKYLLNLENIDIEKSWRENFNELDPFINYIFENKRIFDLV
                     LFQSAGTKYQDIVEKITSFVTKKTVCCLDKLKTIGMINQNLVFNEEEIHFYIYSFYAT
                     FYDILKHSYSKEKTMLLTRRLYQFSVPGWIELLS"
     gene            3091..3759
                     /locus_tag="IXT20_002016"
     CDS             3091..3759
                     /locus_tag="IXT20_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HAQ4860452.1"
                     /translation="MLKINPLTLITLNIFFPVIIFLGKGNTFQVGCFIIATVLVLMFR
                     KIKTCIYFILIYSVLFLVMKIIDAIKIEFFTIFFGTSLYIVFRMLPVVMISWLLVSVY
                     TSNELLSSLEKIHLPKKIMLSITVVIRFFPTYRSEIRMIKESLKMRNIRLSFFQPLQY
                     LEFLLVPVLIRATMIAEEMTANAITKGIESPVKRSSYYKTNMSLLDYIILFSVTIFFL
                     SILI"
     gene            3761..5182
                     /locus_tag="IXT20_002017"
     CDS             3761..5182
                     /locus_tag="IXT20_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HAQ4860453.1"
                     /translation="MKELFEFNNVSFSYKDVKEQALVDLNFSIKKGSLTLITGSSGSG
                     KSTLLKLMNGLIPRLYDGKLVGEILFENNSLQSLTQESISKNIGYVSQDPRGQFFTTN
                     TTSELVFSMENFGFTKEHMDKRINELAELLDLSNILDKNIFSLSSGERQKISIGCSLS
                     LNSKLIILDEPSSNLDFKATKKLADLLCDLKNKGFTIVIAEHRFYYLKELIDQVLFVK
                     NRTVKTTTIQLLKTENNNALRKFDIFNSSIEVPYIEKVSADVLKVEEITYENILKDIN
                     FDVKVGDVIGLVGKNGVGKTTLLRILMKIIRPTKGKIIENKKISSPFLVMQEMDYQFF
                     TESVRSELALGNEIVSKDQQEKILKKMELYKMKDQLPFDLSGGEKQRLLVSIASLSKT
                     NLFLFDEPTSGLDYINMERIADLIKDLKEKGAVVIATHDPEFLYKTCNRIIYLKNGGI
                     KKDIKLRVEDSTLIEQIFNDITK"
     gene            5200..5769
                     /locus_tag="IXT20_002018"
     CDS             5200..5769
                     /locus_tag="IXT20_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MptD family putative ECF transporter S
                     component"
                     /protein_id="HAQ4860454.1"
                     /translation="MKTKDYIFIGLATVISLVIYFFSIMVSSIGGAFGHSISPGIFGL
                     LSGIIFVYISYNYPRKGIFTIYTIVLLLFFTLMGGAYLPWFISSISMAILADIILSVF
                     GYDRAIPQVVSWVLMQLGSAAGQWIPIWFFTDRFRQDWIDRGQSAATMDAMIHYAVGI
                     WGIISVLVVASLSMIGVLIGRKVLKKYKK"
     gene            5879..7633
                     /locus_tag="IXT20_002019"
     CDS             5879..7633
                     /locus_tag="IXT20_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ4860455.1"
                     /translation="MKVYKKLFSYVPKMKYYGWLATLFSDFSVVLTVYGYYSIYKFLQ
                     SLIIVGDEYLAKSYAVQTVAWLTLGALFYIFSGLFSHILGFRLETNLRKKGISGLTEA
                     SFRFFDLNSSGYIRKTIDDNAAKTHMAIAHLIPDSAQAIVTPVLSVALGFFINFKIGL
                     VLIAMLVIGVFLLKKMTENTNFIQKYQESLDVLSSETVEYIRGIQVIKIFGAKVTSFK
                     ALNKAINDYAKFAYKYSLSGKTPFVLFQWLFFGIVCILIVPITFFITSLTTPKILAVD
                     LLMLFFLSGIIFVSFMRIMYVSMHLFEARYAVETLDSLYLEMKQDQLKYGNETQIKNC
                     DIAFENVSFSYKEECVLENFNLVLEQKRIYALVGKSGSGKSTIAKLISGFYNVDKGQI
                     KIGGKPLNHYSKDTIINTIAFVFQDTKLFNQSIYDNVSIANPQAPASKVFDALRLAGC
                     ESILEKFPEREHTLIGSKGVYLSGGEKQRIAIARAILKDAKIVIMDEASASIDPDNEY
                     ELQKAFQYLMKEKTVIMIAHRLSSIKSVDEILVLSEGKIVERGNHNELMAKHRVYKQL
                     VTNYENANDWRLDNEKFL"
     gene            7587..9359
                     /locus_tag="IXT20_002020"
     CDS             7587..9359
                     /locus_tag="IXT20_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002841847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ4860456.1"
                     /translation="MRMQMIGGLTMKNFYKKKFALTDQGAEALTKASISSFFVYCINM
                     VPAFIIMMLIDELVLENAKPRWLYFAVSFVTLLFMYWLLDREYENLYNSTYKESAHLR
                     VQIADDLSNLPLSYFSKHNLSDLSQTIMSDVEGIEHAMSHAIPKAGGMALFFPFISVM
                     LLVGNVKMGLAVILPTLFSFVLILLSKKSQTKANTKYYDTLRENSEEFQETIELQQEI
                     NSFNLSKKVQDRLFKKMEESERIHLKVELSTFSVMALSSIFSYVSLAVVILVGVHLLL
                     TGEVTILYVVGYLLAAIKIKDSFDSMKEAVLEIFYLAPKIQRIRAMKETSIQEGSDSP
                     LKSFDIELRDVSFSYDNNTPILDHISFTAKQGEVTALVGASGSGKTSILKLVSRLYDY
                     DEGCILIDGYDIKRVSPASLFSKIAIVFQEVTLFNTSILENIRIGNSQASDEEVKKAA
                     RLANCEDFIEKLPDGYHTLVGENGSSLSGGERQRLSIARAFLKNAPILILDEITASLD
                     AENEKRIQESLNRLIQDKTVLIISHRLKSIEKVNKIVVMDQGKVVDQGTHSELYRRSE
                     IYKNLIKKTKLSEKFVYEKEAQSR"
     gene            9882..10151
                     /gene="rpsN"
                     /locus_tag="IXT20_002021"
     CDS             9882..10151
                     /gene="rpsN"
                     /locus_tag="IXT20_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HAQ4860457.1"
                     /translation="MAKKSKIAKAKKQMAMIEKYADKRQELKAAGDRTALAKLPRDSN
                     PNRLRLRDQTDGRPRGYMRKFGMSRIKFRELAHQGLIPGVKKASW"
     gene            10166..10345
                     /gene="rpmF"
                     /locus_tag="IXT20_002022"
     CDS             10166..10345
                     /gene="rpmF"
                     /locus_tag="IXT20_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018345355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HAQ4860458.1"
                     /translation="MAVPARKTSKAKKRLRRTHQTVVKPEISFDEANGDYRRSHHVSL
                     KGYYNGKKVIKGASK"
     gene            10377..10754
                     /locus_tag="IXT20_002023"
     CDS             10377..10754
                     /locus_tag="IXT20_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4860459.1"
                     /translation="MEIIYPPLVEEGLKYYLETTQQSLDKCTFYRSMVERGIITETGL
                     PTQQAIENGLVKDYYEDQGLSFDDFLRIYPIFEEYDEELFQCIDGYWEIPVDMKENLV
                     SQLESGELTFEDAQQIQAYLEDR"
     gene            10775..10924
                     /gene="rpmG"
                     /locus_tag="IXT20_002024"
     CDS             10775..10924
                     /gene="rpmG"
                     /locus_tag="IXT20_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003550836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HAQ4860460.1"
                     /translation="MRQNIILECVETGERLYLTSKNKRNNPERLELKKYSPKLRRRAI
                     FKEVK"
     gene            10948..11931
                     /locus_tag="IXT20_002025"
     CDS             10948..11931
                     /locus_tag="IXT20_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HAQ4860461.1"
                     /translation="MGIPVTIVSGFLGAGKTTLINQALQVSPYPREEIIIIENEFGEV
                     GIDHKLLVHSEERVLQLNNGCMCCSLRSDLLAILISLLETIEEHQQPISQIIIETTGI
                     ADPQPIIQTLLTAPQLRGRVYIDSLLTVIDSDSFELIEREPQALKQLVMADRIFVSRK
                     QNLNLSALKKLQREIKSINPLSDIRMFTHSETIQKKDFYNLKKFNHPLVFKSDSSENH
                     YNGKYYHQHHQVHEFKSILLTSDQDIKKDFLLQWIDWLILTNQGSLYRFKGLIALQEQ
                     DFIVAMQEVNEQVNFQYTTRQKDQTTIILIGKMLDEERIKQSFDKLLAESK"
     gene            11961..12083
                     /locus_tag="IXT20_002026"
     CDS             11961..12083
                     /locus_tag="IXT20_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HAQ4860462.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKEHKRNKMKKLA"
     gene            12101..13624
                     /locus_tag="IXT20_002027"
     CDS             12101..13624
                     /locus_tag="IXT20_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HAQ4860463.1"
                     /translation="MKKISVGLIGIAALGLLGACSSTNDAKVSNDKDGKLEIVTTFYP
                     MYDFTKNIVGDEANVDLMVPAGSEPHDYEPSAKDMAKAHDADVFVYHNENMESWVPKA
                     KESWKKAGPNVVEGTKDMILLPGSEEEDHDHGEEDHHHELDPHTWVSPKMAIKEVSNI
                     KDQLVKLYPKKAKVFETNAEKYLTKLKRLDADYTTSLKEAKQKSFVTQHAAFGYLALD
                     YGLKQVPIAGLSPEEEPSSGRLAELKEYVKKNKINYIYFEKNANDKIAKTLANEAGIK
                     LEVLNPLESLTKEQMDNGEDYVSVMEDNLKALEKTTMVAGEEVVPEKEAKDEKTVANG
                     YFKDADVKDRELSDYTGEWQSVYPLLKDGILDEVFDYKAKLNKDMAAAEYKDYYTTGY
                     KTDIDTINIKDNTIDFVVNGEHHQYTYKYVGYKILNYEKGNRGVRFNFETDDAGAGRF
                     KYIQFSDHGIAPSKAEHFHIFLGGESQEKLYNEMHNWPTFYPASLSKHEIAQEMMAH"
     gene            13648..13887
                     /locus_tag="IXT20_002028"
     CDS             13648..13887
                     /locus_tag="IXT20_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4860464.1"
                     /translation="MVRKVLLSMDSKNIDAYRRYRFAKQMRLNGSTLADIAATLHVSI
                     DTVHRVLKRDPDCCFIKVNTGRSRRLAKRHNSLAN"
     gene            complement(14080..14715)
                     /locus_tag="IXT20_002029"
     CDS             complement(14080..14715)
                     /locus_tag="IXT20_002029"
                     /inference="COORDINATES: protein motif:HMM:NF033563.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAQ4860465.1"
                     /translation="MVRHLFVDFQWSPEEISNRLKHEHMDFSISFITIYRAICNDLFD
                     TEPLSHGNKGLIRSLHHRGKTRHTKNHVESRGKICLTHSIHGRPKSADDRSDFGHWKA
                     DIVAGKTGGTCLVTLTDRKSCFLLAGKKVPRKKAAYVSEKMIELLSSIPNEAVKTITP
                     DRGKEFSNHFAVTEALNNMPFYFPDPHALGNNEQIKIRIDSFENTCQNRKR"
     gene            complement(14736..14954)
                     /locus_tag="IXT20_002030"
     CDS             complement(14736..14954)
                     /locus_tag="IXT20_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ4860466.1"
                     /translation="MSQYRHLTILERERIFLLHEEGFSISIIGNDIQHSPFTILHELR
                     RNKKKTIHLPKRKKIMLIEEKNADVITF"
     gene            15072..16688
                     /locus_tag="IXT20_002031"
     CDS             15072..16688
                     /locus_tag="IXT20_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002406986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HAQ4860467.1"
                     /translation="MKKIPITILTGYLGSGKTILLNHILYGDHGKEIAVIVNDIGEVN
                     VDGVLVDKKGFKRTGEKLVSMPNGCICCTLREDLIVELDQLVQFSDLNQIVIEASGIS
                     KPIPIIQAIILAKNPLDIDLTQRLEIGAIIIVVDVKRFWDNFETGEKIQERKAEGNVV
                     EEEQDIRGLLIDQFECCNILLLNNSDLVSTKTIYKINGLIAKLQSNAKIIRTIKGEVD
                     LSKILNTNLFNIDETSYSVGWFKELELGYQNHIPETEEYGISSFIYRNRLLLDAQKFA
                     HLTDKNFPGNITRAKGFLWFAQHSDFSTIFETSGSYKSIESLNYWVASLPKIECQMII
                     QKNPELKEAWHPLFGERINKLVFIGMKMDQQCIKEQLEGCLTDSLNADNFRKISPFPW
                     KKSNMFSQISLMKFLRKAGVKIGDVIIIGILIITSFIPLFVFSIEKENETTEDAIKYA
                     IVRIDGKEMDRFDLDKINHKLVNYHPAKGQYNIVEIKDGRIRVKEDNSPDQIAVRTGW
                     ISKPGQTSICLPHKLVISIEKKESTDYYIY"
     gene            complement(16929..>17216)
                     /locus_tag="IXT20_002032"
     CDS             complement(16929..>17216)
                     /locus_tag="IXT20_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAQ4860468.1"
                     /translation="EDYKELPEHYQPVFTTFKKYRTEIKRALRVPYSNGPIECLNNHI
                     KVLKRIAYGFRNFQNYRERIFLYREKYFKKTKNTTQLTKARTTTRLDKIAV"
BASE COUNT         5984 a         2397 c         3269 g         5566 t
ORIGIN      
        1 ggaataatat gcttcaaaat tttattaatg gtctgtatga ctttcacttt ttacagaatg
       61 ctctaattac atctgtagtt atagggattg tttctggaac agtaggttgt tttattattt
      121 taagaggaat gtctcttatg ggtgatgcta tttctcatgc tgttttgcca ggagtagcaa
      181 tttcttatat tttaggtatt aattttttta ttggtgcgat tgtatttggt ctattagctt
      241 ctatcattat tacttttata aaaagcaata gtgtaataaa aggagataca gcaattggta
      301 ttactttcag ttctttttta gcattaggag ttatccttat aggagttgca aatagctcaa
      361 cggatttatt tcatatctta tttggaaata ttttagctgt acaagatatt gataagtgga
      421 ttacaattgg cgtttctgtt ttagtgttgg ttacagttgt tctatttttt aaagaattat
      481 taatcacttc ttttgatcca ttaatggcaa aagcaatggg gatgaatgtc agtttttatc
      541 attatctatt aatgattcta ttaactttag tagcagtaac agctatgcaa agtgtgggaa
      601 ctatattaat tgttgcaatg ttaattacac cagctgctac agcatattta tatgcaaaca
      661 gcttaaaaac tatgatattt atttcggcga cagtcggagc gtgctcatca ttgctaggtt
      721 tgttcattgg ttatagtttt aacatagcag caggatcaag tattgtttta acagcggcat
      781 tcttatttgt aattagtttt ctaatctcac caaaacaaaa atttttaaga aagaaggaga
      841 gaaagttatg atgagaaaat ggaaagcagt actgggaagt ctggggattt tgattgctct
      901 ttttatattc ggggcatgtt caacaaatag taaagacaaa gatacagtgg cttcgaacga
      961 aaaattaaag gtagtagtta ctaattcgat tttagcagat attactgaaa atatagcaaa
     1021 agataaaatt gatttacaca gtatcgtacc tattgggaaa gatccccacg aatatgaacc
     1081 tttgcctgaa gatgttcaaa aaacttcaaa agcagatttg attttttata acggtgttaa
     1141 cttggaaact ggaggaaatg cttggtttac aaaattagta aaaaatgcga acaaagagga
     1201 aaacaaagac tattttgcag caagtgatgg catagatgtt atttacttag agggtcagag
     1261 tgagaaaggg aaggaagatc cccatgcttg gttaaattta gaaaacggta ttatttacgc
     1321 taaaaatatt gaaaaacagt tagcggaaaa agatcctgat aataaaaaat tctataaaga
     1381 aaatctagat aagtatattg aaaaattgga ttctctagac aaagaagcta aatctaaatt
     1441 tgcttcaatt ccgaatgata aaaaaatgat tgttacaagt gaaggatgct ttaaatattt
     1501 ctcgaaagcg tataatgtgc cttctgctta catttgggaa atcaacactg aagaagaagg
     1561 aacaccagat caaataaaac acttagttga aaaattacgc acaacaaaag ttccctcctt
     1621 attcgtagaa agcagtgtgg acgatagacc aatgaaaaca gtatcaaaag ataccaatat
     1681 tcctatctat tcaacgattt ttactgattc aattgcagaa aaaggacaag atggtgatag
     1741 ttactatgcg atgatgaaat ggaacctgga taaaattgct gaaggccttt cgaaataatt
     1801 tggagggtag gtatggtaca aattaaaaca actactatac taatttattt actagcaatt
     1861 caggaattat caaaaagaaa aaagaaatta aaaaataatg atttagcaaa agttctaagt
     1921 gttagtccag catctgtaag tgagatgctg actaaactta gatatgagga ctatttagat
     1981 ataggatttc aattaactga taaagggaaa aaatttattg acgattacaa aaaagttttt
     2041 aagtaaaaga ctttattttt agggaggata agaaaatggc tattggagtg attatgatag
     2101 gacttatcct aatagctgga tataattttt ttaaatcttt ctcggtttat tttagaaaag
     2161 gtgaatgatt tagatgagtc tattaattct tgtaggattg ctttttgtat cagttatttt
     2221 gctctttctc attagtttag ctctaaggaa tgagttcagt aatagacgtt aacgattgaa
     2281 acttcaagtc ctgtgtaaga taatatagtt aattcatctt attaggagga agtacccata
     2341 gaaaccagag attatattac aactcaccaa aaattaatag aaagtggact aagccatttt
     2401 gaaaaagaag gatttcagca aactaagata aggcaactgt gcttgaccgc aggcgtaact
     2461 acgggtgctt tttataaaca ttttacttct aaggaagcgt tatttgaaga aattattcag
     2521 ccgcatatta atgcgcttat taatatgtat aatgaagagt ttaccaaata cctcttaaac
     2581 ttagaaaata tagatataga gaaaagttgg agagagaact tcaatgaatt agatccattt
     2641 attaattata tttttgagaa taaacgtata ttcgatttgg ttttattcca atcagctgga
     2701 accaaatatc aagatattgt tgaaaaaatt acatcatttg tgacaaaaaa aacagtctgt
     2761 tgtctagata agttaaagac tatcggaatg atcaatcaaa atttagtctt taatgaagaa
     2821 gaaatccact tctacattta ctcattctac gctacattct atgatatatt gaagcatagc
     2881 tattcaaaag aaaagactat gctattaacc agaagattgt atcaattttc tgttcctggg
     2941 tggattgaat tactgagtta attaaaaaaa gcttgcattg agaattgttc tcagttatgc
     3001 tttttgataa gatcgcactg ttatctgaat aacggtgcat ttttatcagc ttaagataac
     3061 agtgcgatct tattttttgg aggtgaaaga gtgttaaaaa tcaatccgtt aaccttgatt
     3121 actttaaata ttttttttcc ggtcattatt tttttaggaa agggaaatac atttcaagtg
     3181 gggtgtttca ttattgctac agttttagta ttgatgtttc gtaaaataaa gacatgtatt
     3241 tatttcatct tgatttattc agttctgttt ttagtaatga aaattattga tgctatcaag
     3301 atagagtttt ttactatatt ttttgggaca tccttatata ttgtatttcg aatgttgccg
     3361 gtcgtaatga tctcatggtt gttggttagt gtctatacta gcaatgaatt attatcctct
     3421 ttagaaaaaa tacatttacc gaaaaaaata atgttaagta ttacagtagt gatcagattc
     3481 tttccgactt atcgttctga aataagaatg attaaagaat ctttaaaaat gagaaacatt
     3541 cgcttgtctt tttttcagcc tctgcaatat ttggaatttc tgttagttcc cgttcttata
     3601 cgtgcaacta tgattgctga agaaatgacc gctaatgcaa taacaaaggg gatagaatct
     3661 cctgtgaaaa gaagttctta ctataaaaca aacatgagct tattagacta tatcatcctg
     3721 ttttccgtta ctattttttt cttaagtatt ttgatttgag gtgaaagaat tgtttgaatt
     3781 taacaatgtt tctttttcat ataaggatgt taaggaacaa gcgcttgttg accttaattt
     3841 ttcaataaaa aaaggcagtc ttacattgat tactggttct tctgggagtg ggaaatccac
     3901 actattaaag ttaatgaatg gacttattcc aagactttat gacggaaaat tggttgggga
     3961 aattttattt gaaaataact cgctacaatc cttaacacaa gaaagtatat caaaaaatat
     4021 tggctacgta agtcaagatc ctagaggaca attctttaca acaaatacaa cgagtgagtt
     4081 agtattttcg atggaaaact ttggatttac aaaagagcat atggataaaa gaatcaatga
     4141 attagctgaa ttattagatc tttcaaatat attagataaa aacatttttt cgctatcaag
     4201 tggtgaacgg cagaaaattt ctattggatg tagtttatcg ctaaactcta aattaattat
     4261 attggatgaa ccgtcttcaa atttggactt caaagcgact aagaaacttg ctgacttact
     4321 ttgtgattta aaaaataaag gatttactat tgttatagct gaacatcgtt tttactattt
     4381 gaaagaatta attgatcaag ttctatttgt taaaaataga actgtaaaaa ctacaactat
     4441 tcagttgtta aaaactgaaa acaataacgc attaaggaaa tttgacattt ttaatagttc
     4501 gattgaagtt ccctatatcg aaaaggtaag tgcagatgta ttaaaagttg aagaaattac
     4561 ttatgaaaat attctaaaag acataaattt tgatgtcaaa gttggagatg ttattggttt
     4621 ggtagggaaa aacggtgtgg ggaaaacaac cttactaaga atactcatga agattattcg
     4681 ccctaccaaa ggaaagataa tcgaaaataa aaaaatttca tcgccgtttt tagtaatgca
     4741 agaaatggat taccaatttt tcacagaatc agtgaggagc gagttagccc tggggaatga
     4801 aatagttagt aaagatcagc aagaaaaaat tttaaaaaaa atggaacttt ataaaatgaa
     4861 agatcaatta ccgtttgatt tatccggtgg agaaaaacaa agattattgg tttctatagc
     4921 ttcattgtca aaaactaatt tatttctatt tgatgaacct acaagtgggt tggattatat
     4981 caatatggaa aggattgctg accttattaa agacttaaaa gaaaagggtg cagtagttat
     5041 agccactcat gatccggaat ttctttataa aacatgcaat agaattattt atttaaaaaa
     5101 tggtggaata aaaaaagata taaaacttag agtagaagat tcaactttga tagaacagat
     5161 ttttaatgac ataacgaaat agaattgagg agaatatata tgaaaacaaa agattatatt
     5221 tttattggtt tagctacagt tatttcccta gtaatttact ttttttcaat aatggttagt
     5281 agtattggtg gggcgtttgg tcacagcata tccccaggga tttttggatt attatctgga
     5341 atcatatttg tttatattag ctataactac cctagaaaag gaatttttac tatttacaca
     5401 attgttttat tgttattttt tacgttaatg ggcggggcgt acttaccttg gtttatcagt
     5461 tcgattagta tggcgatctt agctgatatt attctatctg tatttggata tgatcgagca
     5521 attcctcaag ttgtgtcttg ggttctaatg caactaggca gtgctgccgg acagtggatt
     5581 cccatatggt tctttacaga tcgttttcgt caagactgga tagatagagg acaatctgct
     5641 gcaacaatgg atgcaatgat ccactacgcg gtgggtatat ggggaattat ttcagtgtta
     5701 gttgtagcaa gtttatctat gattggtgta ttgattggaa gaaaagtctt aaaaaaatac
     5761 aaaaaataga acgtagttta ccttctatct gtttgtacat ctctaattta cgctattacg
     5821 gaaagcatta acagttgtaa acgaataaga gaagaaattg aatgcggggg aggaaaaaat
     5881 gaaagtatac aaaaaattat ttagttatgt ccctaaaatg aaatactacg gatggttagc
     5941 cactttattt tctgattttt ctgtcgtatt aacagtctat ggatattaca gtatttacaa
     6001 atttcttcag tctctaatta ttgtaggaga tgaatattta gctaagagtt atgcggttca
     6061 aactgttgct tggttaactt taggagcttt attttatatt ttttctggct tattttctca
     6121 tatccttggg tttcgcttag agactaattt gagaaaaaag ggaattagcg gtttaacaga
     6181 agcaagtttt cgtttttttg atttaaattc ttcgggatat attcgaaaga caattgatga
     6241 caacgcggct aaaacacata tggcaattgc tcacttgatt ccagatagtg ctcaagccat
     6301 agtaacacct gttttatcag ttgccttagg ctttttcatt aactttaaaa ttggattggt
     6361 tctaatcgcc atgttagtaa ttggtgtatt cttgttaaag aaaatgacag agaatacaaa
     6421 ttttattcaa aaatatcaag aatcgttaga tgtgttaagc tctgaaacgg tagaatatat
     6481 ccgcggaatt caagttataa agatatttgg tgctaaagta acatcattta aagccttgaa
     6541 taaagcaatt aacgattatg ctaagtttgc atataagtat tctttgagtg ggaaaacacc
     6601 ttttgttctt tttcaatggc tattttttgg catagtttgc attttgatag ttcctataac
     6661 cttttttata acttctctta ccactcccaa gatattagct gttgacttgt tgatgttgtt
     6721 ttttttaagt ggaattattt ttgtttcttt catgagaata atgtatgtaa gtatgcactt
     6781 gtttgaagca agatatgcag tcgaaacact agactccctt tatttagaaa tgaagcaaga
     6841 ccaactaaaa tatgggaacg agacacagat taaaaattgt gacattgcat ttgaaaatgt
     6901 cagcttttca tacaaagaag agtgtgtttt agaaaatttc aacttagttt tagaacaaaa
     6961 aagaatttat gcattagtag gaaaatcagg aagcggaaag agtacgatag ctaaactaat
     7021 ttcaggtttc tacaatgtgg ataagggaca gattaaaatt ggtggtaagc ctttaaatca
     7081 ctattcaaaa gacacaatta ttaacacaat tgcttttgtc tttcaagata cgaaactatt
     7141 caatcagagt atttacgata acgtttctat agcaaatcct caagcgccag caagtaaagt
     7201 atttgacgcg ctaagattag ccggttgcga atcgatttta gaaaaatttc ccgaaagaga
     7261 gcatacctta attggtagta aaggagttta tctgtcagga ggagaaaaac aacgtatagc
     7321 tattgccaga gcaattttaa aagatgcaaa gattgttatt atggatgaag ccagtgcatc
     7381 tattgatcca gataatgagt atgaactaca aaaggctttt caatatttga tgaaagaaaa
     7441 aacagttatt atgatagctc atcgattatc cagcattaaa tctgtagatg aaattcttgt
     7501 cttgtcagaa ggaaaaatag tagaacgtgg aaatcataat gaattaatgg ctaaacatcg
     7561 agtgtataaa caattggtca ctaattatga gaatgcaaat gattggaggc ttgacaatga
     7621 aaaattttta taaaaaaaaa tttgctttaa ctgatcaggg cgcagaagct ttaactaaag
     7681 cttctatttc tagttttttt gtatactgca ttaatatggt accagcattc attataatga
     7741 tgctgataga cgaattagtt ttagaaaatg caaagccccg ttggctttat tttgcagtat
     7801 cgtttgttac tttgcttttc atgtattggt tgttagatag ggagtatgaa aacttatata
     7861 atagtactta taaagaaagt gcgcatttaa gagtgcaaat tgcagacgat ttgtcaaatt
     7921 taccattatc ctatttttca aaacataatt tatcagattt atctcaaact atcatgtctg
     7981 acgttgaagg tattgagcat gcgatgagtc atgcaatacc taaagccggc ggtatggctc
     8041 tgtttttccc ttttatttca gtgatgcttt tggttggtaa tgtcaaaatg ggattagctg
     8101 ttattttgcc aacgttattt agttttgtct taatcttgtt atcaaagaaa tcccaaacga
     8161 aagccaatac taaatattac gatactttga gagaaaactc ggaagaattt caagaaacta
     8221 ttgaattgca gcaagagatt aatagcttta atctatctaa aaaagttcaa gacagacttt
     8281 tcaaaaaaat ggaagagagt gaaaggattc atttaaaggt agaattaagt actttttcag
     8341 tcatggcctt atcctctatt ttctcatatg ttagtttagc agtagtcatt ctagtaggtg
     8401 ttcacttact gttaacgggt gaagtgacta tactctacgt cgttggttac ttactagccg
     8461 caataaaaat aaaggattcc ttcgattcta tgaaagaagc agtgcttgaa atattttatt
     8521 tagctcctaa aatacagcga attagagcta tgaaggaaac cagtatccaa gagggatcag
     8581 atagtcctct gaaatctttt gacattgagt tgcgagatgt ctctttctca tatgataaca
     8641 ataccccaat tttagatcat atctctttta cggctaaaca aggagaagtg actgcgttag
     8701 taggtgctag tggttccggg aaaactagta tcttgaaatt agtgtctaga ctgtatgatt
     8761 atgatgaagg ctgtatttta attgatgggt atgatattaa acgtgtatct ccagcttctt
     8821 tgtttagcaa aatcgctatt gtttttcaag aggtaacttt gtttaataca agtattttgg
     8881 aaaatatacg tattggaaat agtcaagcgt cagatgagga agtgaaaaaa gcagctcgtt
     8941 tagctaactg cgaagacttt attgaaaaac tacctgacgg ttatcataca ttggtcggag
     9001 aaaacggaag ttcactttct ggaggagagc gacaacgcct atccatagca cgggcttttt
     9061 tgaaaaatgc gccaatttta attttagatg aaattactgc cagtttagat gcggaaaatg
     9121 aaaaacgtat tcaggaaagt ttaaatcgtt taatacaaga taagacagta ctaattatat
     9181 cgcatcgctt gaagtctatt gaaaaggtaa ataaaattgt agtgatggat caaggaaaag
     9241 tagttgatca aggtactcat agtgagttgt atcgaaggtc agaaatatac aaaaatctca
     9301 taaagaaaac taaactatct gagaaattcg tctatgaaaa agaagctcaa tcacgatgat
     9361 gaaataggct ttaaattaac ctacaatttt tgggatatag ttataattct ttttcaaaaa
     9421 gaataatttt attaaacagc atctaatttt gaatacacga aagaatcagt atgattgaat
     9481 tagttcactt aatgtctatt cttataatat aaggaataat caaagtttta aaaaattggc
     9541 gataaaccag ttaaacataa aagattgaat aaaaagttat agtttctttc atattgccgt
     9601 tttaagtaga ggaaaagcta cttaaaacgg tatttgtgtt tgattggact aataaaagtg
     9661 aattcttatt tttcagagct taaactgcat attcttttaa atgatttgat tatctctatg
     9721 taccgataaa ttcgttttca aatagttatc tattcatatg agttttattt catgttattt
     9781 attgttgaca agagcaactc ttgttggtaa agtatcagtg tcaaaacgta acgattacga
     9841 tttgagatgt ctaaatcaaa ataaaaggag tataaaggta tatggcaaaa aagtcgaaaa
     9901 ttgctaaagc aaagaaacaa atggcgatga ttgaaaagta tgcagataaa cgtcaggaat
     9961 taaaagccgc aggagatcgc accgctctcg ctaaattgcc aagagattca aatccgaatc
    10021 gtctgaggct tcgtgatcag acggatggtc gtccaagagg gtatatgcga aagttcggta
    10081 tgtcccgtat taagtttaga gaacttgccc atcaagggtt gattcctgga gtaaaaaaag
    10141 caagttggta aaaggaggaa atatcatggc agtaccagca agaaaaacat ctaaggcgaa
    10201 gaaacggtta agaagaactc atcaaactgt agtaaaacca gaaatttcat ttgacgaagc
    10261 gaatggtgat tatagacgta gccatcatgt atctttaaaa gggtactata atggaaagaa
    10321 agtaattaaa ggagcttcaa aatagtttaa tatataactt ttaggaggga atcaaaatgg
    10381 aaattattta cccaccttta gttgaagaag gattaaagta ttatcttgaa acgacccagc
    10441 aatcgctaga taaatgcact ttttatcgtt cgatggtaga gagaggaatt attactgaaa
    10501 cgggtttacc gacccaacag gcaattgaaa atggattagt caaagattac tatgaggacc
    10561 aaggcttatc atttgatgat tttttaagaa tctatccaat ttttgaagag tatgacgagg
    10621 agttatttca atgtatcgat ggttattggg agatacctgt tgatatgaaa gaaaatttag
    10681 tttcacaatt ggaatctggc gagttaactt ttgaagatgc acaacaaatt caagcatatc
    10741 ttgaagatcg ataagtattg aaggaggaag atttatgcgt caaaatatta ttttggaatg
    10801 tgtcgaaact ggagaacgtc tgtatctcac cagcaaaaat aaacggaata accctgaacg
    10861 gttagaatta aaaaagtatt cgccaaaatt gcgcagacgg gcaattttca aagaagtgaa
    10921 atagctaagt ggtaaaaagg tgacaaaatg ggcataccag ttacaatcgt ttctgggttt
    10981 ttaggggctg gaaaaacaac gctaatcaat caagccttgc aagtctcacc ctaccccagg
    11041 gaagaaatta ttataattga aaatgagttt ggtgaagttg ggattgatca caaattatta
    11101 gttcatagcg aggaacgggt gttacaacta aataatggat gtatgtgttg cagtcttcgc
    11161 agtgatttat tagcaatatt gatttcttta ctggaaacaa tagaagaaca tcaacaacca
    11221 atttcacaaa tcatcattga aactacaggt atagcagatc cccaaccaat cattcaaaca
    11281 cttttaacag cgcctcaact aagaggaaga gtatatatcg atagtttatt gactgttatt
    11341 gatagtgaca gttttgaact tattgaaaga gaaccacaag cccttaagca acttgtcatg
    11401 gcagatcgca tttttgtgtc tagaaaacaa aacttgaatt tatctgcact aaaaaaatta
    11461 cagagagaaa tcaaaagtat taacccttta tccgatattc gaatgttcac acatagtgaa
    11521 acgattcaaa aaaaggattt ttataatttg aaaaaattta atcatccctt agtttttaaa
    11581 agtgattcat cagaaaatca ctataatggc aaatactatc atcaacacca tcaagtacat
    11641 gagtttaaat caattctctt aacttcagat caagacataa aaaaagattt tttactccaa
    11701 tggattgact ggctgatttt aaccaaccaa gggagcctgt atcgttttaa aggattgatt
    11761 gctttacaag agcaggattt tatagttgcc atgcaagagg taaacgaaca ggttaatttt
    11821 caatacacca ctcgacaaaa agaccaaacc actattattc taatcggaaa aatgttggat
    11881 gaagaaagga tcaaacagag ttttgataaa ttactggcgg aaagcaaata atgataagta
    11941 gtgattgggg gtaatttcta atggagaaaa cggatatatc tagtgcatat cgacgactta
    12001 aaagcccgaa tattaaaact agaaaaaggg cattaaaaat tatcaaagaa cataaacgaa
    12061 ataaaatgaa gaaattagca taattaagga aggaaaaaga atgaagaaaa tcagtgtagg
    12121 acttattggg atagcagctc taggattatt gggagcttgc tccagcacta atgatgcaaa
    12181 agtatcgaat gataaagatg gaaaattaga aattgtaaca acattctatc caatgtatga
    12241 tttcaccaaa aacatcgtcg gcgatgaagc gaacgtggat ttaatggttc cagcgggatc
    12301 agaacctcat gactatgagc cctcagctaa ggatatggca aaagctcatg atgctgatgt
    12361 ttttgtatat cataacgaaa acatggaatc atgggtacca aaagctaaag aaagttggaa
    12421 gaaagctggg cctaatgtag tagaaggaac aaaagacatg attttattgc caggtagtga
    12481 agaagaagat catgatcacg gagaagaaga tcaccatcat gaactagatc ctcatacgtg
    12541 ggtttcaccc aaaatggcaa ttaaggaagt ttcaaatatc aaggatcagt tagttaaatt
    12601 gtatccgaaa aaagcaaaag tatttgagac aaatgctgaa aagtatttga ctaaactgaa
    12661 acggttggat gctgactata ccactagtct taaagaagcg aaacaaaaaa gttttgtgac
    12721 gcaacacgca gcctttggat atttagcttt ggattatgga ttgaaacaag ttcctattgc
    12781 tggactaagt cctgaagaag agccatcttc tggtcgatta gctgaattaa aagagtatgt
    12841 taaaaaaaat aagatcaatt atatttactt tgaaaagaat gctaatgata aaattgcaaa
    12901 aactttggcc aacgaagctg ggataaaatt ggaagtactg aatcctcttg aaagtcttac
    12961 aaaagaacaa atggataatg gtgaagatta tgtttcagta atggaagaca atctaaaagc
    13021 gttagaaaaa acaactatgg ttgctggtga agaggtagtt ccagaaaaag aagcgaagga
    13081 tgaaaagaca gttgctaatg ggtactttaa agatgccgat gtgaaggatc gcgaattgag
    13141 tgattataca ggcgaatggc aatctgttta tccgttgtta aaagatggaa ttttggatga
    13201 agttttcgat tacaaagcaa aactcaataa agacatggca gctgccgagt acaaagatta
    13261 ttatactact ggctataaga cagatattga tactatcaac attaaagaca acaccattga
    13321 ttttgtagtc aacggtgaac atcatcaata cacatacaaa tatgttggat acaaaatttt
    13381 gaattatgaa aaaggaaacc gtggtgttcg ttttaatttt gaaactgatg atgcaggtgc
    13441 tggtcgtttt aaatatattc aatttagtga tcatggaatt gcaccaagta aggcagagca
    13501 tttccatatt ttccttggtg gagaaagtca agaaaaactc tataatgaaa tgcataattg
    13561 gcctactttc tatccagctt cattgtctaa acatgaaatt gctcaagaaa tgatggctca
    13621 ttaaaatcca atttttggag gaaatacatg gtaagaaaag tattattgtc tatggattcc
    13681 aagaatattg atgcttatcg gcgttatcga tttgcaaagc aaatgagatt aaatgggagt
    13741 acattagctg atattgctgc aactttacat gttagtattg acacagttca tagagtatta
    13801 aaacgggatc ctgattgttg ttttatcaag gtcaatactg gaagaagtag acgtttagca
    13861 aaacggcata atagcttggc caattaattg ataataccag agtagatcat ttttccctta
    13921 tttggatttg atctgctttt tattatgtct tgaattgtca aatcaagcgc aacactttat
    13981 caaagaagac ttcataaggt gttctccaat ctaaacactt tctaggtctg ttattcagtt
    14041 tttcaatcat ctgatttatc gttgagttat ctaccgtagc tatctctttc gattttggca
    14101 agtattctcg aatgagtcta ttcgtatttt aatttgttca ttgttgccaa gggcatgagg
    14161 atctggaaaa taaaagggca tattattcaa tgcttctgta actgcgaaat gattggagaa
    14221 ttcttttcct ctatctggag tgatcgtttt gaccgcctca tttggaatac tgcttaaaag
    14281 ttcaatcatt ttctcgctca cataagcggc tttctttctg gggaccttct ttcctgctaa
    14341 aaggaagcat gattttctgt ctgtcaaagt cactaaacaa gtaccaccag ttttgccagc
    14401 tacaatatcg gctttccaat gaccaaaatc acttctatca tctgcggatt ttggacggcc
    14461 atggatcgaa tgagtaaggc aaatctttcc acgagactcg acatgatttt ttgtatgtct
    14521 tgtttttcct cgatggtgca atgaacggat caggccttta ttgccgtgtg agaggggttc
    14581 tgtatcgaaa agatcattgc aaattgcacg gtagatcgtg ataaaactaa ttgaaaagtc
    14641 catatgttca tgcttcaaac gattcgagat ttcctctggc gaccactgaa aatcgacaaa
    14701 taaatgacga acaattgacc aaactcacgg atcatttaga atgtgataac gtccgcattt
    14761 ttttcttcga taagcataat ttttttccgc tttggaaggt gaatagtttt tttcttgttt
    14821 cttctcagtt catgtaaaat agtaaacggg ctatgctgaa tgtcgttgcc aattatacta
    14881 atagaaaatc cttcttcgtg aagcagaaag attctttctc gttcgagtat agtaagatga
    14941 cgatactggc tcataattat tttccttcta ggagtgttct cgacaaactt atactagttg
    15001 gatatcgatt atgactcatt tttatgtgtt gcacttaaag tgtaaattca aggtatagaa
    15061 agaggttccc tatgaaaaaa atacctatta caatcttaac tggctatctt ggttcaggaa
    15121 aaacgatact tctaaatcat attctgtacg gtgatcacgg aaaggaaatt gctgtcattg
    15181 taaatgacat aggggaagta aatgttgatg gggtattagt cgacaagaaa ggttttaaaa
    15241 gaactggaga aaaacttgtt tcgatgccga atggatgcat ttgttgtaca ttacgcgaag
    15301 atttgattgt tgaactagat caattagtgc aattttctga cttgaatcaa attgttattg
    15361 aagcttcagg aatcagcaaa ccaattccaa ttattcaagc aattatatta gctaaaaacc
    15421 ctttagacat tgatttgacc caacgattag agattggtgc cattattata gtagtggatg
    15481 taaaacgttt ttgggataat ttcgaaacgg gtgaaaagat tcaggaaaga aaagctgaag
    15541 gtaatgtagt tgaagaagaa caggatattc gtggcttgtt aattgatcaa ttcgagtgtt
    15601 gtaacatatt acttttaaat aatagtgact tagtgtcaac caagacgatt tacaaaatta
    15661 acggtctaat tgctaagtta cagtccaatg ccaaaataat taggacaatt aaaggtgaag
    15721 tagacttatc gaaaattcta aatacaaatc tttttaatat agatgagact tcctattcag
    15781 tgggctggtt caaagagtta gagcttggct atcaaaatca tattcctgaa acagaagaat
    15841 atggtatctc aagttttatc tatcgaaatc ggttactcct tgacgcacaa aaatttgccc
    15901 atcttacgga taaaaatttt cctggcaata ttacaagagc aaaagggttt ctttggtttg
    15961 cccaacatag tgatttttcg acgatctttg aaacatcggg tagctataaa agtattgaat
    16021 cattaaatta ttgggtagct agtttaccta aaattgagtg tcaaatgatt atacaaaaaa
    16081 atcccgaatt gaaggaagcc tggcatccgt tgtttggtga acgcattaat aaactagttt
    16141 ttattggtat gaagatggat caacaatgta ttaaggaaca acttgagggt tgtttaactg
    16201 attctcttaa tgcagataat ttccgaaaaa tttcaccatt tccatggaaa aagtctaata
    16261 tgtttagcca gatatcgtta atgaaatttt taagaaaagc tggtgtcaag ataggcgatg
    16321 tcataattat tggcattttg attattacct cttttattcc cttatttgta ttctctatag
    16381 aaaaggagaa tgaaacaact gaagatgcta tcaaatatgc catcgttcga attgatggca
    16441 aggaaatgga tcgcttcgat ttggataaga tcaatcataa actcgtgaat tatcatcctg
    16501 cgaagggaca atataatatt gttgagatta aagatggaag aattcgagtt aaagaagata
    16561 atagtcctga tcaaatcgcg gttagaactg gatggatctc aaaacccggt caaacgagta
    16621 tttgtttacc ccataagtta gttattagta ttgagaaaaa agaaagcaca gattactaca
    16681 tttactaatc tggaaataga tgaggaggtt tttaataaga aaaagatatt attattaata
    16741 atagcagggg ctttattaag tgtgttaagc gcttgtggac aagaaaaaga agcggtgaca
    16801 acaggcacaa gtatctttaa cgggacctgc aatggtttag ttaacagtcg gttagattac
    16861 ttattttggc tcttcgtcaa tgagtgttgg tgaagaaatt caatagaata atcgagatac
    16921 tcacacggct agacagctat cttgtctagc cgtgttgttg ttctggcttt agtcagttgg
    16981 gtagtgttct tagttttctt gaaatatttt tctcgatata aaaagattcg ttctcggtag
    17041 ttttgaaaat ttctaaagcc ataggcaatt cgttttagta ctttgatatg gttgttcaaa
    17101 cattctatag ggccgttgga atagggaacg cgcaacgctc gtttgatttc tgttcgatat
    17161 ttcttgaagg tcgtgaacac tggttggtag tgctctggca actccttgta gtcttc
//
LOCUS       DACGLE010000059        17007 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0534
            SAMEA3304449-rid9560453.denovo.063, whole genome shotgun sequence.
ACCESSION   DACGLE010000059 DACGLE010000000
VERSION     DACGLE010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304449
            Sequence Read Archive: ERR985064
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17007)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17007)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702225.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702225.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 20:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,045
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 27 of 67
            Pseudo Genes (incomplete)         :: 25 of 67
            Pseudo Genes (internal stop)      :: 19 of 67
            Pseudo Genes (multiple problems)  :: 4 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17007
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304449-rid9560453.denovo.063"
                     /strain="VREN0534"
                     /db_xref="taxon:1352"
     gene            complement(<1..126)
                     /locus_tag="IXT20_002033"
     CDS             complement(<1..126)
                     /locus_tag="IXT20_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EntF family bacteriocin induction factor"
                     /protein_id="HAQ4860469.1"
                     /translation="MEEKNRLNAKQCSDQELKKIKGGAGTKPQGKPASNLVECVFS"
     gene            complement(230..541)
                     /locus_tag="IXT20_002034"
     CDS             complement(230..541)
                     /locus_tag="IXT20_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HAQ4860470.1"
                     /translation="MKKNAKQIVHELYNDISISKDPKYSDILEVLQKVYLKLEKQKYE
                     LDPSPLINRLVNYLYFTAYTNKIRFTEYQEELIRNLSEIGRTAGINGLYRADYGDKSQ
                     F"
     gene            complement(543..740)
                     /locus_tag="IXT20_002035"
     CDS             complement(543..740)
                     /locus_tag="IXT20_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterocin"
                     /protein_id="HAQ4860471.1"
                     /translation="MKHLKILSIKETQLIYGGTTHSGKYYGNGVYCTKNKCTVDWAKA
                     TTCIAGMSIGGFLGGAIPGKC"
     gene            complement(1134..2861)
                     /locus_tag="IXT20_002036"
     CDS             complement(1134..2861)
                     /locus_tag="IXT20_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAQ4860472.1"
                     /translation="MNNLLNYQLPVANWVENITEWFTTTFSGLFSFLQTIGQAVMSGI
                     TNLLLVIPAPLFILLLTVAAFFISKKRPGLTLFTLIGLWFIYNQGLWNDLMNTVTLVL
                     LSSVISIIIGVPLGILMAKSSKAQSIIKPILDFMQTMPGFVYLIPAVAFFGIGMVPGV
                     FASVIFALPPTVRFTNLGIRQVPKELVEASDSFGSTSRQKLFKLELPLAKSTIMAGIN
                     QTTMLSLSMVVIASMIGAPGLGRGVLSALQRAQVGNGFVNGVALVILAIIIDRFTQHL
                     NQPNNKKAAGATTQKSKKRQGLIIGAVVVVILGAIGIGSFSSAKETKRINLSYVEWDT
                     EVASTNVVGEVLKQMGYDVTMTPLDNSIMWKSVSNGESDAMVSAWLPKTHGSQYAQYK
                     DQVEDLGANLTGAKVGLAVPAYMDVNSIDELTDQAGKKIIGIEPGAGVVTAAENTIQK
                     YDNLKDWKVETSSSGAMTVALGQAIKKHEPIVVTGWTPHWMFAKYDLKYLEDPENGMG
                     SEEQIHTMVRKGLKEDQPEAYKVLDNFHWSEKDMEKVMLEINNGKDPQQAAKDWIKEN
                     QELVESWKK"
     gene            complement(2854..4047)
                     /locus_tag="IXT20_002037"
     CDS             complement(2854..4047)
                     /locus_tag="IXT20_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein"
                     /protein_id="HAQ4860473.1"
                     /translation="MVKVEVKHLTKIFGKKTQAALDMMNDHQPKTEILKKTGATVGVY
                     DVNFDVKEGEIFVIMGLSGSGKSTLIRLLNRLIEPTSGSIYIDGEDVSKLSKEELREV
                     RRHKINMVFQNFGLFPHRTILENTEYGLEVRGVPKEERQEKAEKALENSSLLSFKDQY
                     PSQLSGGMQQRVGLARALANDPEILLMDEAFSALDPLIRREMQDELLDLQANVQKTII
                     FITHDLNEALRIGDRIALMKDGEIMQIGTGEEILTNPANDYVREFVEEVDRSKVLTAQ
                     NIMVPALTTNIESDGPNVALTRMRNEEVSMLMAVDRKRHLKGIITADQALEARKQKRP
                     LIDFLDENVTVIGKDMVVSDIFNIIYDSPTPLAVVEDGKLKGVVIRGSVIEALAETSE
                     VSEHE"
     gene            complement(4234..4878)
                     /locus_tag="IXT20_002038"
     CDS             complement(4234..4878)
                     /locus_tag="IXT20_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ4860474.1"
                     /translation="MSGKKKIVRPRYQQIAVDLAERIVENRYKVGEKIHARSTLASTY
                     NASPETTRKAINVLVDLGIMEVRHGSGAFVASKEKAKDFLLQYQDVQSIQDTKAEIMA
                     SVEKQQDELNHFSQLLDQLVNQTKRIHKMNPLLPFELSLSEKAMHLDRSISELNIWQA
                     TGATVIAISHNEELLVSPGPYAKFTAGDIVHFIGNEYTLQRMTNFFYPEHQLAE"
     gene            complement(4972..5106)
                     /locus_tag="IXT20_002039"
     CDS             complement(4972..5106)
                     /locus_tag="IXT20_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HAQ4860475.1"
                     /translation="MATFLLSVLIFGSAGAIVYTRLKSGKNCDDCQTACPVKKEQIKR
                     "
     gene            complement(5108..7240)
                     /gene="feoB"
                     /locus_tag="IXT20_002040"
     CDS             complement(5108..7240)
                     /gene="feoB"
                     /locus_tag="IXT20_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HAQ4860476.1"
                     /translation="MNIALVGNPNSGKTSLFNLLTGSNQYVGNWPGVTVEKKEGSLKK
                     NPNIHIQDLPGIYSLSPYTPEEVIARDYLVQEQPELIINIIDATNLERNLYLTTQLLD
                     LDIPMIIGLNMMDVIKKEGKKINLEKLSYGLGVPVLPISVLKKQGIEKLIEKIQESAK
                     KRPSIPVPCTYDPRLEAALHEIEDVLGEQSGKKRWYAMKYFERDQKVREDYEIAVSQQ
                     KEIEQLIQLTEKLLDDDSETILVNERYEFITQLCTLCVVSNDSFQLSMSDKIDQIATN
                     RWLALPIFAFVMWLIYYLAIQTVGTMGTDWINDTLFGTWLPEHVGRLLAEWQVAGWMQ
                     ELILNGIIAGVGAVLGFLPQLIILFLCLSFLEDCGYMARIAFVMDRLFRKFGLSGKSF
                     IPMLIATGCGVPGVMASRTIENEQDRRLTIMVTTFMPCSAKLPIIALVAGAFFPHQSW
                     VAPSAYFLGMTAIIFSGISLKKTRLFAGDTAPFIMELPAYHFPQWSAVLRQTYDRSKS
                     FVKKAGTIIFVSSIVIWFSSSYNFYAQPVSEDQSILAFFGRILAVVFQPLGWGNWQGT
                     VAAITGLVAKENIIGTFGILFGHAEVSENGREIWQVLQHTYTPAAAYSLLAFNLLCAP
                     CFAAIGAIHREMNAKKWTWIAIGYQCGLAYLVSFVIYQLGHLLEGGQVALGTYLAILL
                     MIGLVYVLFRQPKSKNQFYTILSLEGEE"
     gene            complement(7237..7710)
                     /locus_tag="IXT20_002041"
     CDS             complement(7237..7710)
                     /locus_tag="IXT20_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HAQ4860477.1"
                     /translation="MQTLAQAKQNFVYQVERIETEEKTKRHLQNIGIVAGSKIVVVNL
                     SGNNAILLVKSHRVAVSREILGQIFVNKKTASSESWTSLDQLKAGETGIVVAIHGQGA
                     VKRRLMDMGLTRNVKIVIRKLAPLGDPIELTVRGYELTLRKSEAELILIQREEKE"
     gene            8111..8335
                     /gene="nrdH"
                     /locus_tag="IXT20_002042"
     CDS             8111..8335
                     /gene="nrdH"
                     /locus_tag="IXT20_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HAQ4860478.1"
                     /translation="MNVKIFSKNNCMQCKMVKRFLTENQIAFEEVNIDEQPEAVDWLK
                     AQGFQSVPVITSDAETVIGFRPDQLRKLAS"
     gene            8494..10653
                     /gene="nrdE"
                     /locus_tag="IXT20_002043"
     CDS             8494..10653
                     /gene="nrdE"
                     /locus_tag="IXT20_002043"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HAQ4860479.1"
                     /translation="MSLKNLQDVSYFKLNNEINRPVNGQIPLNKDKEALVAFFEENVK
                     PNTMTFPSIMEKINYLIDQEYIEAEFIQLYSPSFIEKLYQFLDEQHFSFKSFMAAYKF
                     YSQYALKTNDGSAYLETYEDRVAFNALYFANGDEELALALADEMIHQRYQPATPSFLN
                     AGRKRRGELVSCFLTQVTDDMNSIGRSINSALQLSRIGGGVGITLSNLREAGAPIKGY
                     EGAASGVVPVMKLFEDSFSYSNQLGQRQGAGVVYLNVFHPDIIAFLSAKKENADEKIR
                     VKTLSLGVIVPDKFYELTRKNEDMYLFSPYSVEKEYGVPFSYVNITAEYDHLVANPNI
                     RKQKIKARDLENEISKLQQESGYPYVINIDTANRENPIDGKIIMSNLCSEILQVQTPS
                     VINGKQEYEVLGTDISCNLGSTNIVNLMQSPNFGQSVRTMTRALTFITDASDIDVVPP
                     IQNGNKLNHTIGLGAMGLHTYLAKEQMEYGSEDSLDFTNIYFMLLNYWTLVESNNIAR
                     ERKQVFHNFEKSAYADGTYFEKYVTGEFQPKSEKVKALFEGIFIPTAEDWNALKQAVM
                     KDGLYHQNRLAVAPNGSISYINDTSASIHPITRMIEERQEKKIGKIYYPAPYLSNETI
                     PYYTSAYDMDMRKVIDVYATAQQHVDQGMSLTLFMRSEIPEGIYEWKTTTKQTTRDLN
                     ILRHYAFHKGIKSIYYVRTFTDDAEEIGSNQCESCVI"
     gene            10703..11668
                     /gene="nrdF"
                     /locus_tag="IXT20_002044"
     CDS             10703..11668
                     /gene="nrdF"
                     /locus_tag="IXT20_002044"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HAQ4860480.1"
                     /translation="MSETYYEAINWNEIEDIIDKSTWEKLTEQFWLDTRIPLSNDLDD
                     WRTLSQLEKDTVGHVFGGLTLLDTVQSESGMDQLRKDVRTPHEEAVLNNIQFMESVHA
                     KSYSSIFSTLNTKKEIEEIFDWTNTNPYLQKKAERINEIYKHGTPLEKKIASVFLETF
                     LFYSGFYTPLYYLGNNKLANVAEIIKLIIRDESVHGTYIGYKFQLGFNELPEAEQETL
                     KDWMYNLLYELYENEERYTEELYDPIGWTEEVKTFLRYNANKALMNLGMDPLFPDTAN
                     DVNPIVMNGISTGTSNHDFFSQVGNGYLLGHVEAMKDDDYLIGLE"
     gene            complement(11757..12896)
                     /locus_tag="IXT20_002045"
     CDS             complement(11757..12896)
                     /locus_tag="IXT20_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAQ4860481.1"
                     /translation="MQSRFKQSKLFFWTVELVLTIIGVYFILQMPNIFSPILKMLSAI
                     FLPLLVAGFIYYMFDPIVVFLEKHRIPRVFGFLLTFLVVIILVVLTVMNVVPQLMEQT
                     IQLTQSLPMYADETSKWLNELAQREEFKNFNLEEQLASANLSLSNLFNIILVSLSASV
                     SKIISFMMQFFILLFTVPFILLFMFKDGHKFIDALSHFFPKAIRKELRQTVKELNETL
                     SAYISSTILDALIIGVMSFLAMRIFDQPYSLLLAVICGITNIIPYVGPFIGAVPAIIV
                     GLFISPFQALYMALSILVIQQLDGNLIKPLLFGKSMNIHPLTIILVLIGAGSVAGILG
                     MLICIPVYAVIKTIILNIRKIYLLRRMEHVSEKLDSPKVLEETSE"
     gene            complement(13205..13918)
                     /locus_tag="IXT20_002046"
     CDS             complement(13205..13918)
                     /locus_tag="IXT20_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HAQ4860482.1"
                     /translation="MNDMVLTIPEIILRLGIAMLFGGAIGFERQYKNRPAGMRTHILV
                     CMGACIIALIQVEIASGALRDALEYPQLAGTIRSDEARLIAQVVSGVGFLGAGTIIVT
                     KQSVTGLTTAASLWAIAGLGIAIGMGYYSIAIISFIGIAIALTVVKRVIHVPTTKKLE
                     IQFIHRKETKDFLTDYFEEKNIVIEDVNFDVKFVDDYRVYKNIYTIDLPKGLTYADVI
                     EELSVHKNITKLHLVSIAQ"
     gene            complement(14172..14873)
                     /gene="nagB"
                     /locus_tag="IXT20_002047"
     CDS             complement(14172..14873)
                     /gene="nagB"
                     /locus_tag="IXT20_002047"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HAQ4860483.1"
                     /translation="MEIIRVKNAEEGGKKAFEIIKEGMNKGAKVLGLATGSTPVTLYK
                     EMTSSDLDFSEMTSVNLDEYVGLGGSDEQSYRHFMDVQLFDKKPFKETFVPDGKAEDL
                     EAACKKYDQIIDAHPIDIQILGIGQNGHIGFNEPGASFDGTTSVVDLTESTINANKRF
                     FDKAEDVPTKAVSMGIGSIMKSKEIVLMAFGESKADAIKGMIEGPVTNHLPASVLQNH
                     DNVVVIIDEEAASKL"
     gene            15107..16639
                     /locus_tag="IXT20_002048"
     CDS             15107..16639
                     /locus_tag="IXT20_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAQ4860484.1"
                     /translation="MSILTLNNITQQFGDKILYEGVDLQLNAGEHLGLIGQNGAGKST
                     LIKIITGEILPDDGQIHWQKNIHKGYLDQYVEVDETLTIEEFLKTAYAKEYEKEEKIA
                     FLYQTYSETFDDSLLEKAGKLQTELDQGVFYQIDTLVAEMSSGLGIDVLGLDSLLKNL
                     SGGQRSKVILAKLLLENPDVLILDEPTNHLDDQHIQWLTQFLQDFDGAYIVISHDQEF
                     LDQITTHIADIEFGKITKYTGHLKQALKQKEQNRESYLRQYHAQQKQIEKTEAYIRKY
                     KAGSRSTMAKSRQKQLDKIERLTPPASASKAVFDFPFSPIVTTLAVETTDLVIGYDRP
                     LLAPINLTIRFGENIAIRGFNGIGKSTLLKTLIGEIEKISGDFHFPDNTKINYFSQDL
                     YWENPLETPLQYLSSQFPKATIKELRRQLAKAGLVNQLASEPLTTLSGGEQTKVKLAQ
                     LTMNQGNLLILDEPTNHIDQETKESLQDGIQKFPGTVIIVSHEQEFYQDLVQRVIEIE
                     PK"
     gene            complement(16830..>17007)
                     /locus_tag="IXT20_002049"
     CDS             complement(16830..>17007)
                     /locus_tag="IXT20_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ4860485.1"
                     /translation="VFMFMYFIGGAAGSLLGTTMWQQYGWLGVTVSGLVFQGCAFFFQ
                     TVVFKGKRNLENKK"
BASE COUNT         5278 a         3495 c         2910 g         5324 t
ORIGIN      
        1 tgaaaaaaca cattccacta aatttgatgc cggttttcct tgaggttttg ttccagcacc
       61 tcctttaatt tttttaagct cttgatctga acattgttta gcatttagtc tatttttttc
      121 ttccattacg attcccccta caaactataa atacttttat gtcactgatt caatatacct
      181 atttttctaa tcaaaaaatt ttgagacata tttaatatta taagtatcat taaaattgag
      241 atttatctcc ataatctgct cgatataaac catttattcc agcagttctt ccaatttcac
      301 tcaaatttct tattaattcc tcttgatatt cagtgaatct tattttatta gtataagcag
      361 taaaatatag ataattcacc aatctattta ttaaaggact gggatctaat tcatattttt
      421 gtttttctaa ttttaaatat accttttgta aaacctcaag aatatcagaa tatttaggat
      481 ctttacttat agatatatca ttatataatt catgaacaat ttgcttagca ttttttttca
      541 ttttagcact tccctggaat tgctccacct aaaaaaccac ctatagacat tcctgcaata
      601 caagtagttg ccttggccca atcgaccgta catttatttt tagtgcaata cactccattt
      661 ccataatatt ttccactatg agtggtaccc ccatagataa gttgtgtctc tttaatagac
      721 aaaattttta aatgtttcat aatctaatca cccctaaatt aatgttttta gactattatt
      781 ctaacattca ttaaatttca aaaaacaatt ccttcttgaa acgtagcaaa ataattcctg
      841 aataagaatt taatcacttt taagtatata aactatgtat attctttaat attttttgag
      901 aatgcacttc gtacataata aactttaatc acattagtct ttcataccta cgatcgatct
      961 ctttgactcg cacccataaa ttcataatag tttgtcggcg tctttgaacg tttcgaagat
     1021 gagctacgca ttctcttttt ttatagacaa aaaagataga gacgaacatg gagcttccaa
     1081 atttggatat ttgatccaat tttggaagct ccatttattt cgtgtctgtc tttttatttt
     1141 ttccagcttt cgactaattc ttggttctct ttgatccagt cttttgctgc ttgctgtgga
     1201 tcttttccat tgttgatttc caacataact ttttccatat ctttttctga ccaatggaaa
     1261 ttgtctaata ctttgtaagc ttctggctgg tcttctttca atccttttcg aaccatcgta
     1321 tggatctgct cttcgcttcc cattccgttt tcaggatctt ctaagtattt cagatcatat
     1381 ttggcaaaca tccaatgagg tgtccatcct gtaactacaa ttggttcgtg ttttttgatc
     1441 gcttgaccta gtgcaacggt cattgctcct gaagaagatg tctctacttt ccagtctttc
     1501 aagttgtcat atttttggat cgtgttttca gcagccgtaa ctacaccagc tcctggctcg
     1561 attcctatga ttttcttacc tgcctgatcg gttaactcat cgatcgaatt tacatccatg
     1621 taggctggca ctgctaaacc aacttttgct cctgtcaaat tggcacctag gtcttcgact
     1681 tgatctttat attgtgcata ttgtgaacca tgagttttag gtagccaagc tgaaaccatg
     1741 gcatctgact cgccatttga aacagatttc cacatgattg aattatctaa tggcgtcatc
     1801 gttacatcat agcccatttg tttcaatact tcaccgacta catttgttga agcaacttct
     1861 gtatcccatt caacataaga taagttgata cgttttgttt ctttggcaga tgaaaaactc
     1921 cctataccaa tcgcacctaa gatcactact actacagcac caatgatcag tccttgacgt
     1981 tttttacttt tctgtgttgt tgctcctgca gcttttttat tgtttggctg gttcaaatgc
     2041 tgtgtaaaac ggtcaatgat gattgctaag ataactaatg caaccccgtt gacaaatcca
     2101 ttcccgactt gtgctctttg caatgctgat aatacgccgc gtcctaagcc gggtgcaccg
     2161 atcatagagg caataacgac cattgaaagt gacagcattg ttgtttgatt gatccctgcc
     2221 atgatcgtac ttttagctaa aggcaattct agtttgaata gtttttgacg acttgtgctc
     2281 ccgaatgaat cagaagcttc tactaattct ttaggaactt gtcgaatccc taaattcgta
     2341 aaacgaacgg ttggtggcag agcaaagatg actgaggcaa atacccctgg aaccatcccg
     2401 ataccgaaaa atgcaactgc tggaatcaaa taaacgaatc ctggcattgt ctgcataaag
     2461 tcaaggatcg gtttgataat actttgggct ttactgcttt ttgccatcaa gatccctaat
     2521 ggtaccccaa tgatgatcga gatcacacta gataaaagaa caagtgtgac agtattcatc
     2581 aaatcattcc atagaccttg gttataaata aaccatagac cgattaatgt gaacaacgta
     2641 agtcctggac gtttcttaga aataaagaaa gctgctaccg tcaataacaa gataaacaaa
     2701 ggagctggaa tcactaacag taaatttgta atccccgaca tcactgcctg tccaatcgtt
     2761 tgtaaaaatg aaaataggcc agaaaaagtt gtcgtaaacc attctgtgat attttctacc
     2821 cagtttgcca ctggtaattg atagttcaat agattattca tgttcactca cctcgcttgt
     2881 ttctgctaat gcttcaatca cactacctcg gatgacgaca ccttttaatt ttccatcctc
     2941 tacaactgca agtggagtcg gtgagtcata aataatattg aaaatatcac ttaccaccat
     3001 gtctttccca atcaccgtca cattttcatc taggaagtcg atcaatggac gtttttgctt
     3061 cctagcttct aacgcttgat ctgcagtgat gatccctttc aggtgtctct ttctgtcaac
     3121 agccatcagc atgctgactt cttcattacg catccttgtc aatgcaacgt ttggtccatc
     3181 agattcgata ttcgtcgtca atgcaggtac catgatgttt tgagccgtaa gcactttgga
     3241 acgatcgact tcttccacaa attcacgtac ataatcattt gctggattgg tcaatatttc
     3301 ttcacctgtt ccgatctgca tgatttcgcc gtctttcatc aaggcgattc tgtccccaat
     3361 ccgtaaagct tcattcaaat catgggtaat gaaaataatc gttttttgaa cattggcttg
     3421 caggtccagt aattcgtctt gcatttctcg ccggatcagc ggatcaagag ccgagaacgc
     3481 ttcgtccatc agcaagatct caggatcatt tgctaaagct ctggctaggc caacccgttg
     3541 ttgcatccca ccagataact gactaggata ttggtcttta aatgaaagca aactagaatt
     3601 ttctaatgct ttttctgctt tttcttgtcg ttcttcttta ggtacaccac ggacttctaa
     3661 cccgtattct gtattttcca agatcgttcg atgtggaaac agaccgaagt tttggaaaac
     3721 catattgatt ttatgacgac ggacttctcg caattcttct ttgcttaact tcgatacatc
     3781 ttctccatca atatagatac tgcccgaagt tggctcaatc agacgattca atagacggat
     3841 caatgtggat ttcccactcc cggaaagccc catgatcacg aagatttctc cttcttttac
     3901 gtcaaaattc acgtcataca caccaaccgt tgcaccggtt ttctttaaaa tctccgtttt
     3961 cggctggtga tcattcatca tatctaaagc ggcttgtgtc tttttaccaa atatctttgt
     4021 taaatgtttt acttctactt ttaccaaaca aattctcctt ttttatgtaa attcattttt
     4081 ctttctgaaa aaagtgacca cactatgtta acatcatgta gtcactttgt caaaaatgaa
     4141 agaaatttgc ttgtccaaac tgaaaaaaac ttggctaaat ctttgtaaat aaaaggttta
     4201 gccaagttaa tattttttta atatttcaca caatcattct gcaagctgat gctccgggta
     4261 aaaaaagttt gtcatacgct gtagtgtata ttcatttcca ataaaatgaa cgatgtcccc
     4321 tgccgtaaat ttagcatagg ggccaggtga aacaagcagt tcttcattat gagaaattgc
     4381 gataactgtg gcgccagtcg cctgccagat attcaactcg ctaattgaac ggtccagatg
     4441 cattgctttt tctgataaag ataattcaaa cggaagtaaa ggattcatct tatgtattcg
     4501 cttcgtttga ttgaccagct gatccaataa ttgagaaaaa tgattcagct catcttgttg
     4561 cttctccaca cttgccatga tttctgcttt tgtatcttgg atggattgta catcttgata
     4621 ttgcaatagg aaatcttttg ctttttcctt cgaagcaaca aatgccccac ttccatgccg
     4681 tacttccatg attccaagat ctaccagcac attgatagcc ttgcgggtag tctcaggaga
     4741 agcattgtac gtactggcaa gtgttgagcg ggcatggatc ttctcaccca ctttatatcg
     4801 gttttcaacg atacgttcgg ctaaatcaac agcaatctgc tggtagcgcg ggcggacgat
     4861 ttttttcttt ccagacattt tctttcctct ttcttttttt actttccctc atcttactat
     4921 gctctagcaa aataagcaaa aaaggggaga atagcctctt gcaacgactg atcatcgctt
     4981 gatctgttcc ttttttactg ggcaagctgt ttgacagtca tcacaattct taccggattt
     5041 taaacgagta tagacaattg cgcctgcact tccaaagatt aacacactta ataagaatgt
     5101 cgccatccta ttcctctcct tctaatgaca atatagtata aaactggttc ttggattttg
     5161 gctgacggaa caaaacataa acgagtccta tcatcagtaa aatagcaaga tacgtcccta
     5221 acgctacctg tccaccctcc aataagtgtc ccaactgata aatcacaaaa ctaactaaat
     5281 aagctaaacc gcattgatag cctatggcga tccaagtcca tttcttggca ttcatttccc
     5341 gatggatagc cccaattgcc gcaaaacaag gcgcacaaag taaattgaag gctaacaatg
     5401 aataagctgc tgcgggagta tatgtgtgtt gtagcacttg ccagatttct ctgccatttt
     5461 cggatacttc cgcatgacca aacaaaatac caaatgtgcc aataatattt tctttagcga
     5521 ccaatcctgt aatcgctgcg actgttcctt gccagtttcc ccaaccaagc ggctgaaaaa
     5581 caactgccag gattcgcccg aaaaaggcta agatactttg gtcttcagaa acaggttgag
     5641 cataaaaatt ataagaagag ctgaaccaga tcacgatact agaaacaaag ataatcgttc
     5701 ctgctttttt aacaaaagac ttgctgcggt cataggtttg tcttaggacc gctgaccatt
     5761 gaggaaaatg atatgcgggc aattccatga taaaaggagc tgtatctcca gcaaacaaac
     5821 gtgttttttt caagctgatt ccagagaaaa taattgcagt cataccaaga aaataggcag
     5881 aaggagctac ccaagattga tgaggaaaaa aggcccctgc tactaaggca ataatcggca
     5941 actttgcaga acaaggcatg aaggttgtga ccatgatcgt caaacgacga tcttgctcgt
     6001 tttcaatcgt gcgactggcc atgacgcctg gcactccaca tcctgtagca atcagcatag
     6061 gaatgaagga ttttcctgaa agaccgaatt tcctgaaaag tcgatccatg acaaaagcga
     6121 tcctcgccat atatccgcag tcttccagaa agctcaagca taaaaacaat ataatcaatt
     6181 gcggtagaaa tcctaacact gctcccacgc ctgcgatgat gccgttcaag atcagctcct
     6241 gcatccatcc cgctacttgc cattcagcta acaatctgcc aacatgttct ggtaaccatg
     6301 taccaaacaa tgtgtcattg atccaatctg tccccattgt tcctactgtt tgaatagcca
     6361 gataatagat cagccacatc acaaatgcga agatcggcaa ggctaaccaa cgattcgttg
     6421 caatctgatc gattttgtca ctcatcgata attggaaaga atcatttgac acaacacaga
     6481 gtgtacatag ttgggtaata aattcataac gttcattgac cagaatcgtc tcactatcgt
     6541 cgtccaatag tttctctgtt aactggatca gttgctcgat ctctttttgc tgagaaactg
     6601 ctatctcgta atcctctctt accttctgat ctcgttcaaa atatttcatt gcataccagc
     6661 gtttcttgcc gctttgttct cctagtacat cttcaatttc atgtaaagct gcttctaaac
     6721 gtggatcata tgtacaggga acaggaatag aaggccgctt ttttgcagac tcctgaatct
     6781 tttcgattag tttttcgatt ccttgctttt tcaatacgct gattggtaaa actggtacac
     6841 caagaccata ggaaagcttt tctagattga ttttttttcc ttcttttttg atgacatcca
     6901 tcatattcag accaatgatc ataggaatgt ctaaatccaa taactgcgta gttaaataaa
     6961 gatttcgttc taaattggta gcatcaataa tgttgatgat cagttcaggc tgttcctgaa
     7021 ccaaataatc tcttgcaatc acttcttctg gtgtataagg agacaaagaa tagatgccgg
     7081 gtaggtcctg aatatgaatg ttcggatttt ttttcaacga tccttctttt ttttctactg
     7141 ttacgcccgg ccaattccct acatattgat tcgaaccagt caagagattg aacaagctag
     7201 ttttcccgct gtttggattt ccgactaacg cgatattcat tctttttcct ccctttgtat
     7261 caagattagt tcggcctctg actttcgtaa cgtcagttca taccctcgca ctgtcagttc
     7321 gattggatct cccagagggg ctagttttcg gataactatt tttacatttc gcgtcaatcc
     7381 catatccatt aaccgtcgct tcactgcgcc ttgtccgtgg attgctacga cgatcccagt
     7441 ctctcctgct tttaattgat caagagaagt ccagctttca gaggaagcag tctttttatt
     7501 gacaaagatt tgacctagta tttcccgact tactgccact cgatgacttt ttaccagtaa
     7561 aatggcattg ttgcctgata aattgacgac gacgatttta cttccggcaa cgatcccgat
     7621 attttgcaaa tggcgttttg ttttttcttc tgtttcgatt cgttcgactt gataaacgaa
     7681 attctgcttt gcctgcgcta aggtctgcat attgattcct ctcttgtgaa ataaatcatg
     7741 tattaaaatc attcattata tgttctaatt ttcttacgtg ttacgcaaat aaatgagaat
     7801 cattctcaca tagaatactc tcggtaattg agaatgtcaa ttatacttgt gatattatga
     7861 gcgaatcttt agtattgata gataaaaatg agaaaaaaat atttcttcat agttttccac
     7921 aattttttct ctattaaatt tgctattttc ccacacgata cttgttttca gctctttttt
     7981 caaaaacaca aaacttagta ttccacagtt tccacaacac aatatgtagt gtttgttgtt
     8041 tgactttttt actaagagtt ttatactatc tattatagat aataagaatg tgttagagga
     8101 ggcactcacc atgaacgtaa aaatcttttc taaaaataat tgtatgcaat gtaagatggt
     8161 caaacggttt ttaacagaaa atcaaattgc ttttgaagaa gtcaatatcg atgaacagcc
     8221 tgaagcagta gactggttga aagcacaagg attccaaagt gttcctgtca ttacttctga
     8281 tgcagaaaca gttatcggat tccgtcctga tcagttgcgt aaacttgcca gctaacatga
     8341 ctaaaagaat caaaacgtga tacatggatt tgaagggctt gagacagcac cagtttttgt
     8401 cgcaaacttc caaatccttt tttaaagctt aaaaacaatg caatgatttg caatcaagca
     8461 tcatcaaata cgaaatgaag gttgggcgta cgtatgagtc ttaaaaattt acaagatgtc
     8521 agttatttca agctgaataa tgaaatcaat cggccagtca atggtcaaat ccctttgaat
     8581 aaggataaag aagctttagt tgcctttttt gaggaaaatg tcaaaccaaa tacgatgact
     8641 tttccatcta ttatggaaaa aatcaattac ttgatcgatc aagaatatat agaagcagaa
     8701 ttcattcaat tgtattcacc atcatttatt gaaaagctat atcaattttt ggacgagcaa
     8761 catttttctt tcaaatcttt tatggctgct tacaaatttt attctcaata tgcgttgaaa
     8821 accaacgacg gttccgctta tctggaaact tatgaagatc gagtagcctt caatgcgctt
     8881 tactttgcga acggggatga agaattagca cttgctcttg cagacgaaat gatccatcaa
     8941 cgatatcagc cagctacacc ttctttcttg aatgctggac gtaaacgtcg gggcgaattg
     9001 gtgtcttgtt tcttgactca agtcactgac gatatgaact ctatcgggcg ctcaatcaac
     9061 tctgctttgc aattgtcccg tattggtggc ggcgtaggga ttactttatc aaatcttcgt
     9121 gaagccggcg ctcctatcaa aggatatgag ggtgctgcaa gtggtgtcgt tccagtcatg
     9181 aaattattcg aagatagttt cagctattct aatcaattag gtcagcgtca aggagctggc
     9241 gtggtttatt tgaacgtttt ccatccagac attattgctt tcctttcagc aaaaaaagaa
     9301 aatgcagatg aaaagattcg cgtaaaaact ctttctcttg gcgtgatcgt acctgataaa
     9361 ttttatgaat tgacacgtaa aaacgaagat atgtatttat ttagtccata tagtgtcgaa
     9421 aaagaatatg gtgtcccatt ctcttatgta aatattacag ctgagtatga tcatctagtt
     9481 gcaaatccaa acattcgcaa acaaaaaatc aaagcgcgtg atcttgaaaa tgaaatctca
     9541 aaattacaac aagaatctgg ctatccttac gtaatcaata ttgatacagc gaaccgcgag
     9601 aatccaatcg atggcaagat catcatgagt aatttatgtt cagagatttt acaagttcaa
     9661 acgccttctg tgattaacgg caaacaggaa tatgaagtat taggaacaga tatctcatgt
     9721 aacttaggct cgacaaatat cgtgaatttg atgcaaagcc ctaactttgg tcaatctgtg
     9781 cgtacgatga ctcgtgcatt gacatttatt acagatgcat ctgatatcga tgtcgtacca
     9841 ccgatccaaa acggaaataa gctgaatcat acgattggat taggtgccat gggcttacac
     9901 acttatcttg caaaagaaca aatggaatat ggctcagagg attcattaga ctttacaaat
     9961 atttacttca tgctgttgaa ttattggaca cttgttgaaa gcaacaatat cgctcgtgaa
    10021 agaaaacaag ttttccataa ttttgaaaaa tcagcttatg ctgacggcac ttatttcgaa
    10081 aaatacgtaa ctggagaatt ccagccaaaa tcagaaaaag tcaaagcatt gtttgaaggc
    10141 atttttatcc caactgctga agattggaat gctttgaaac aagcagtaat gaaagatgga
    10201 ctgtatcatc aaaatcgctt ggcagtagca ccaaatggct caatttcata tatcaacgat
    10261 acgagtgcaa gtattcatcc tatcactcgc atgatcgaag aacgccaaga gaagaaaatc
    10321 ggtaaaattt attacccagc gccttatcta tccaatgaga cgattccgta ttacacttct
    10381 gcttatgata tggacatgcg taaagtaatc gatgtttatg caacggcaca acaacacgtc
    10441 gatcaaggca tgagtttgac tttattcatg cgttctgaaa tcccagaagg catttatgaa
    10501 tggaagacaa caacgaagca aacgacacgt gatttgaata ttttaagaca ttacgctttc
    10561 cacaaaggaa tcaaatccat ttattacgta cgtaccttta ctgatgatgc agaagaaatc
    10621 ggaagcaatc aatgtgaaag ctgcgtgatc taatatcata gatagattcg cttattcata
    10681 ttttaattaa aggagcattc tcatgtcaga aacatattat gaagcaataa actggaatga
    10741 aattgaagat atcattgata aatccacttg ggagaagctg acagagcaat tttggctaga
    10801 tacacgtatc ccactttcaa atgacctaga tgattggcgt acattgtctc aattggaaaa
    10861 agatacagta ggacatgtat ttggcggttt gacactgcta gatactgtcc aatctgaaag
    10921 tggcatggat cagttgcgta aagatgtccg cactccccat gaagaagccg ttttgaacaa
    10981 tattcaattc atggaatccg ttcatgccaa aagttattct tctattttta gtacactgaa
    11041 tacgaaaaaa gaaatcgaag aaatttttga ctggacaaat acgaatcctt atctgcaaaa
    11101 aaaagctgag cgtatcaatg aaatctacaa acacggtaca ccgctagaaa agaaaattgc
    11161 cagcgtcttt ttagaaacat tcttgttcta ttcaggtttt tatactcctc tttattattt
    11221 aggaaacaac aaattggcta acgtagcaga gatcatcaaa ctaatcatcc gagatgaatc
    11281 tgtacatgga acctacatcg gttacaaatt ccaattagga ttcaacgagt tacctgaagc
    11341 agaacaagaa acgttaaaag actggatgta caatcttctt tatgagcttt acgaaaacga
    11401 agaacgttat acagaagaat tatacgatcc aatcggctgg acagaagaag tcaagacatt
    11461 cctacgctat aatgccaaca aagcattgat gaacctaggg atggatccac tctttccaga
    11521 tactgccaat gatgtcaacc caatcgtaat gaacgggatt tcaacaggca caagcaacca
    11581 tgacttcttc tcacaagtcg gaaacggcta tttgttaggt catgtagaag cgatgaaaga
    11641 cgacgattat ttgattggat tggaataagg atttatttta attcaaaaat ttttcgaaaa
    11701 ataaaaagag tttagcgtac agatatttta aatgtacact gaactctttt ttatgcttat
    11761 tcacttgttt cttccagcac ttttggactg tctaactttt ccgaaacatg ttccattctt
    11821 ctcaataaat agattttccg gatatttaat attatcgtct taatcactgc atagacaggt
    11881 atgcaaatca gcatgcctaa tattcctgcg acacttcctg ctccaatcaa taccaagatg
    11941 atcgtcaaag gatggatatt cattgatttc ccaaataaca acggcttgat caaatttcca
    12001 tctaattgtt ggatcactaa aatcgacaac gccatataca aagcttgaaa tggagagata
    12061 aacaaaccga caatgattgc tggtaccgct ccaataaaag ggccgacata cgggataata
    12121 ttcgtgattc cgcaaatgac cgcaagcaac aagctataag gctgatcgaa tattctcatt
    12181 gccaaaaagc tcattacacc aataatcaaa gcatctaaga tcgtactgct gatataggca
    12241 gataatgttt cgtttaattc ttttaccgtt tggcgtaact ctttacgaat cgctttcgga
    12301 aagaagtgag acaacgcatc aataaatttg tgcccatcct tgaacataaa tagcaaaata
    12361 aatggaacgg taaataacaa aataaaaaat tgcatcataa atgaaatgat ttttgagaca
    12421 cttgcagata aactgactaa tatgatattg aacaaattac taagagataa atttgctgat
    12481 gctaattgtt cttccaaatt gaaattttta aactcttccc gctgcgccaa ttcatttaac
    12541 catttgcttg tttcatctgc atacattggc aagctctggg tcaattgaat tgtctgttcc
    12601 attaactgag gcactacatt catgacagtt aaaacaacta aaatgattac gaccaaaaac
    12661 gtcaataaaa aaccgaacac tctcgggatc cgatgctttt cgagaaaaac aacgattgga
    12721 tcgaacatat aatagataaa accagcaaca agcaatggta aaaaaattgc cgaaagcatt
    12781 ttcaatattg gactaaaaat attcggcatt tgtaaaataa agtacacgcc gatgatcgtg
    12841 agtactagct ccactgtcca gaaaaataat tttgattgtt taaaacgtga ttgcatacat
    12901 ctcctccaag gtcctttgat acttctattc tatcatttaa caaagagtta cgcgctgaga
    12961 ttttcatttg cttaaaaaaa acaaaaaaaa taaacatatt ttaatgagta gacgagtact
    13021 taaacaaaaa cacggtttgg agtagtgaca aaaattatac tgcagtccct ttttccaaat
    13081 aaacggtgtt caaaagcttg catcttcgta ataagcccag ccaaacgaaa aatataagga
    13141 actattttta tttggctggg cttattactt gatgtaggac atctatttta caacctcaga
    13201 tcatttattg cgcaatactg actaaatgca actttgtgat atttttgtgt acggacaact
    13261 cctcgatcac atcggcataa gtgagccctt ttggaagatc aatcgtatag atatttttgt
    13321 acactcggta gtcatcaacg aattttacgt caaaattcac atcttcgatt acgatatttt
    13381 tctcttcaaa ataatcggta agaaaatctt tggtttcttt tctatggata aattggattt
    13441 ctaacttttt agtcgtaggt acatggatca cgcgtttcac tactgtgagt gcaatagcaa
    13501 tcccgataaa actgatgatc gcaatggaat agtaacccat gccgatggcg atccctaatc
    13561 cagcaattgc ccataaagaa gcggcagtcg taagtcccgt caccgattgt ttcgtgacga
    13621 tgatcgttcc cgcgcctaag aaaccgacac cactcaccac ttgagcaatc agccttgctt
    13681 catctgaacg aatcgttcca gcaagctgtg gatactctaa agcatctctt aatgcaccac
    13741 tagcgatctc aacttggatc aatgcaatga tacatgctcc catacaaact aaaatatgcg
    13801 tacgcatacc tgctggacga tttttatatt gtcgttcaaa accgattgct ccgccaaata
    13861 gcatagcgat ccctaaacgt aaaataatct ctggaattgt caataccata tcattcatct
    13921 aaacaccctc cagtccaata tatcatgtct gaaaggagca gacaaaaaaa gtgcaatttt
    13981 tacctcttac gaaaaaagga ctgtgacaaa atttttgtca tagtcctttt tatgaataaa
    14041 cggtgttcaa tcagctgacc ctcggtatta ctcaacaaac ggcaaaatat ggggagctat
    14101 ttttcctttg tcttctctaa tactcaggtc aaaacgctga tttcacaacc tctttagcct
    14161 actattgaaa attacaactt acttgctgct tcttcatcga tgatcacaac aacattgtcg
    14221 tgattttgca atacacttgc tggcaaatga ttcgttacag gtccttcaat catccctttg
    14281 attgcatcag ctttgctttc gccaaatgcc atcaagacga tttctttact tttcataatt
    14341 gaaccgattc ccattgaaac agcttttgtt ggtacatctt ctgctttatc gaagaatcgt
    14401 ttgtttgcat tgattgttga ttctgttaag tcaactactg aagtcgttcc gtcaaatgaa
    14461 gcaccaggtt cattgaaacc gatatgtcca ttttgcccga ttcctaagat ttggatatcg
    14521 attggatgtg catcgatgat ttgatcatac tttttgcatg ctgcttccag atcttctgct
    14581 tttccatctg gtacaaacgt ttctttgaat ggttttttat caaataattg gacatccatg
    14641 aaatgacggt agctttgttc atctgaaccg cctagtccaa cgtactcatc taagtttaca
    14701 gaagtcattt cactgaaatc caaatcgctt gaagtcattt ctttatataa agtaactggt
    14761 gtgcttcctg ttgccaatcc taatactttt gcacctttgt tcataccttc tttgatgatt
    14821 tcaaatgctt tttttccgcc ttcttcagcg ttttttacac gaatgatttc catagtgatt
    14881 gctcctttat attttggtat agcccaatta tagcattctt tttttctttt tacaataatt
    14941 agtatagacc aaattgcatt tttttcattt tcttgacaat aaataggaat ccgtgttagg
    15001 tttcataaga tggttcaatc aaacaatcca tgttgcatga aaaccaagca agtgattcac
    15061 tgtggacgct ttatgtctgc agttttttta tgaaaaagga gttattatga gtatactgac
    15121 attaaacaac attacccagc aatttggcga caaaatcctc tatgaaggtg tcgatctgca
    15181 attaaatgct ggcgaacatc ttgggctgat tggacaaaac ggagccggta aaagtacatt
    15241 gattaaaatc atcactggtg agatcttacc agatgacgga cagatccatt ggcaaaaaaa
    15301 tattcacaaa ggttacctcg atcaatatgt ggaagtcgat gaaacattaa caatcgaaga
    15361 atttctaaaa actgcgtacg caaaagaata cgaaaaggaa gaaaaaattg cttttcttta
    15421 ccaaacatat agcgagacct tcgatgattc cttgctggaa aaagctggga aactccaaac
    15481 ggaattagac caaggagtgt tttatcaaat cgacacctta gttgctgaga tgagcagcgg
    15541 gttggggatc gatgtcttag gtttagattc actgttgaaa aatctaagtg gtggacaacg
    15601 ttcaaaggtg attttagcaa aactactttt agaaaaccca gatgttctga tcttagatga
    15661 accaaccaat cacttagatg atcagcatat ccaatggctg acgcagtttc ttcaggattt
    15721 tgacggtgcg tatatcgtga tttcccatga ccaagaattt cttgatcaga tcaccaccca
    15781 tatcgcagac atcgagtttg gaaagattac taagtatact ggccatctaa aacaagcatt
    15841 gaaacaaaag gaacaaaatc gcgaaagcta tttgcgccaa tatcatgctc aacaaaaaca
    15901 aatcgaaaag accgaagctt atattcgaaa atacaaagcc ggttcccgat caacgatggc
    15961 aaaaagccga caaaaacaat tagataaaat cgaacggctg acgcctccag cttcagcaag
    16021 taaagctgtc tttgattttc cattctcgcc tattgtcacg acattagcag tggaaacaac
    16081 tgatttagtc atcggatatg atcgaccgct acttgcaccc atcaatttga ccatccgctt
    16141 tggtgaaaat attgccattc gaggcttcaa cgggattgga aaatctactt tattaaaaac
    16201 actgatcggt gagattgaaa aaataagtgg cgatttccac ttcccagata acacaaaaat
    16261 caattacttt tcacaagatc tgtactggga aaatccatta gaaacaccac tacaatattt
    16321 aagcagtcaa tttccaaaag cgactatcaa agaattacgt cgccaactag ctaaagcagg
    16381 gttagttaat caattagcaa gtgaacctct cactacatta agtggtgggg aacaaactaa
    16441 ggtgaaactt gcacagttga caatgaacca aggtaattta ctgattctcg atgaaccgac
    16501 gaaccatatc gatcaagaaa cgaaagaaag cttacaagac gggatacaga aatttccagg
    16561 aacagtaatc atcgtctctc atgaacaaga attttatcaa gatcttgtgc aacgagtcat
    16621 tgagattgaa ccaaaataaa cgtttgattg aatagttgaa aaaaaggagc tgtgacaaaa
    16681 gttttgtcac aactccttct tctgaataaa tggtggccaa ttagctgacc ttcggtatta
    16741 gctcaaaaaa cggcaaaata tgaggagcta tttttatttg gctattctta ttacttgatg
    16801 cagaacagct tttcacaacc tcttttcgtc tattttttat tttcgagatt tctctttcct
    16861 ttaaaaacaa ctgtttgaaa gaagaacgca catccttgaa aaacaagccc gctaaccgtt
    16921 acacctaacc agccatactg ctgccacatt gtcgtcccta gcaatgaacc agccgcacct
    16981 ccaataaagt acataaacat aaataca
//
LOCUS       DACGLE010000060        16399 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0534
            SAMEA3304449-rid9560453.denovo.064, whole genome shotgun sequence.
ACCESSION   DACGLE010000060 DACGLE010000000
VERSION     DACGLE010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304449
            Sequence Read Archive: ERR985064
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16399)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16399)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702225.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702225.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 20:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,045
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 27 of 67
            Pseudo Genes (incomplete)         :: 25 of 67
            Pseudo Genes (internal stop)      :: 19 of 67
            Pseudo Genes (multiple problems)  :: 4 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16399
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304449-rid9560453.denovo.064"
                     /strain="VREN0534"
                     /db_xref="taxon:1352"
     gene            complement(296..1024)
                     /locus_tag="IXT20_002050"
     CDS             complement(296..1024)
                     /locus_tag="IXT20_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ4860486.1"
                     /translation="MLRIAIVDDDCTIVEGIRHYLLKKVPDIIIDHCYTDGDEAFKNL
                     QNQRFDLLITDITMGKISGLELIESLQQTSNRYFTSIVISAHQNFQYAKKGIDLSIKS
                     YITKPIDFPYLVKLVQEEKMRKSAANQNSLTDDSFNESIQQVIDYVQNNYSEKLTLKD
                     VATQFHYHPAYLGRAFKKETGYSFHDYLLDFRIKRASALLKDTFLSIDEISQKVGIDH
                     VSYFSVLFKKTTGHSPSNYRKKLL"
     gene            complement(1002..2576)
                     /locus_tag="IXT20_002051"
     CDS             complement(1002..2576)
                     /locus_tag="IXT20_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAQ4860487.1"
                     /translation="MKNDLSKVIMRFTYILIFFITLLACFTLFFSYINVIEKEKNQNL
                     QNIQQITHYMETYNRILSGIVTEIYQNYDFQENLNDYYLSSPAEYMEDILSKDRSPTY
                     LVDNLKKLVQDDEVKNISISLDYEKYLSAYAPTKNEKISFIRSLSFISEDMPDATLIL
                     DVSINALKKSLFPTHYNYSLSNGIDSVNLSEDISPSKWKKTQQIAIGNHTLQVYENPN
                     YSSQTLLGLAIMIFLLMSLFILAIHQLLNRSFRNYIQQYQEIITSLNHSSTGDYKSIN
                     IENKVGDLKEIAMHINQFYLDNATYQKTLYENNWLKQEAKYAALQYQIEPHFLFNNLE
                     FIRMTAILNDDEEISDSIYLLSKLYRNNTYQPDIIDLKEELINIDLSLQLHQRRYRHN
                     FDYQLIDHIDQLMLPKLSLQPFVENYIKHGFDSSKKENRLTVYATETTNHYVLKIVEN
                     GKGMDQDKLEHIQQKLNDDIPYSENIGISNSFRRIKHLYPGDTRFVIESQLHVGTTIT
                     IKIQKGGNMHVENCNC"
     gene            2805..3743
                     /locus_tag="IXT20_002052"
     CDS             2805..3743
                     /locus_tag="IXT20_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ4860488.1"
                     /translation="MKLKKGLLGNLKKNRALFIMLLPGALWLFIFCYIPMFGVVIAFK
                     DFRISSRGFFTSLFNSPWVGWENFKYLFASQDAWIITRNTVVYNVVGILLSTFCTVSL
                     AIILSQMANKRLLKKMQTFMIFPNFLSWVVISYFVYAFLSTDKGLINILLSNFGVEPI
                     NWYSEPKYWPFIIVFTSLWKGVGYGSIIYYASIMGIDTSYYEAALVDGASKWQQIRNV
                     VLPHLRSIIVIMLILSVGSIFRADFGLFYQLPRNSGALYDVTNVLDTYVYRGLMNSGD
                     IGMSTAAGLYQSSVGFVLILITNKIVNKLDPSSALF"
     gene            3755..4666
                     /locus_tag="IXT20_002053"
     CDS             3755..4666
                     /locus_tag="IXT20_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAQ4860489.1"
                     /translation="MISKLKRQMYFSLPVERFFTILLILFTLSCIFPFIFVIVISLTD
                     EKALQLNGYELLPAQWSTDAYQYLIQDGGQLLRSLGVTIMITVIGTLITVFMTGTYAY
                     VLSRASFPYRKFFTFYLFFTMLFAGGMVPSYLVMTKMLGLKNTIWALILPLAFSPYNV
                     IILRTFFKKSIPESIIESAKMDGCSEFRVFFQIVLPLAIPGVATIGLFSSLGYWNDWF
                     NALLYIDTNKLIPLQYLLMKIQNSMEFLANNNDITLAQQQAIQNSLPQESTRMAMVVV
                     ATLPIAIVYPFFQKYFVQGLTIGGVKE"
     gene            4690..6162
                     /locus_tag="IXT20_002054"
     CDS             4690..6162
                     /locus_tag="IXT20_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAQ4860490.1"
                     /translation="MKKNMQKYLIGGVSVLTLGLLGACGSSNGEANNETGTTKVTWYQ
                     VGEEPKDFDEVMTKVNEKLKEEINVELDMRLIPDGDYQQKLGVMINSGEEYDICFVNG
                     TDYVNYGNKGAFISLNDHLDKELKDYAAELNEGFIEGGAINGETYGLPVNNDIATTEY
                     WTFNKDLVDQYQLDVENVEGVETLAPVLEKFAEKNTDTNITPIGMTRTSVCIPYDNII
                     GGGVPLAVAFEGDTQTVVNTYETPEMMTYLKAMHEFYKKGYILKDAATVTTDPFRIDS
                     KNWFLGRNSSGYQPLAQKKLEALAGHGLILKPAGKAFKTTGFARVAMHAVSASSKHPE
                     AALKLLNYVNTNQEVADLMVYGIEGKHYDRNEDGSITLTNSDDYYQGAWALVNTYLVS
                     KTSPITEESKKLEEESKKFQNEAPMSPILGFSFDASDFRTEIASVQNIVSQYEVLLSS
                     GTVDPEQYVPELLEKLEKAGLDKIQASAQEQYDEWRKSNK"
     gene            6214..6825
                     /locus_tag="IXT20_002055"
     CDS             6214..6825
                     /locus_tag="IXT20_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HAQ4860491.1"
                     /translation="MNENKWTKGFNWVFQLLLLNFTYFILICLGFGILGFFPATIALI
                     AGVVNRKKYGLQESVKFMSKIYKKNFVRYNLYCWSATLLLAGNITTIWLCWQQSAPLF
                     YLLSTLLSVITLFLVFAMLNWVVIKSLGLWMMGDRLIDYLLFTATMLPTVVMQGLVLG
                     IYFRILMILPVIFLFGGTAVTVILISKLTERALAKLPCQIQSH"
     gene            6855..8768
                     /locus_tag="IXT20_002056"
     CDS             6855..8768
                     /locus_tag="IXT20_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HAQ4860492.1"
                     /translation="MKLTDKQVANIAFIQQLEIPLTQKDEEQIDLIFTDTKANVRQIK
                     ITKNEQQRICLSYSHEDLLYRGLFICLPRFLAQGGEMPYIEETSIIETSYSLDASRNG
                     VMNKETLKKFISYLALMGYNTLYIYTEDTYEVEDLPYFGYLRGAYTKEEVNEIVEFAS
                     GFGIEVVPSIQTLAHLTQFLKWYAQQELMDDQNTLLIGEEKVYAAIEKMIIACKEMYQ
                     TERIHLGMDEAYQAGLGRYLSLYGYCDRTTLMIQHMDRVLSIVDKYKMKPMIWSDFIY
                     KLLDETGKDRYYNPDFKLKALAAKGLPAGLTYVHWDYGSDEVSSYEKVIDNHLKFCDI
                     DHYVLASGAHIWGRLAPNHGKTEAIVSAAVKASKNKGVRSVMLTTWGDEGQETEHWHS
                     LIGALKLADYVYSEEEWNDFEEIYDRLLGTGSYQLMSELRYFDEVDTITNNNLQMASV
                     SKLLLWQDPLVGIYDFHVAAHNKIATRTIGTYYHELAQKLDMNKNWNPKQPILTTIRQ
                     RYILLAEILAQKAELGIYLQHCCLVEDLAGLMELANRCEALANRVNELASIHEIIWYQ
                     TYKTYGWEVLEQRYAALAHRLKTVARRIKNHLQGQDALEELLEERLPFCQYQVPLEIS
                     GFNYRQSSVSGYN"
     gene            8896..11877
                     /locus_tag="IXT20_002057"
     CDS             8896..11877
                     /locus_tag="IXT20_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 42"
                     /protein_id="HAQ4860493.1"
                     /translation="MLSDYYQNLSVISVNTEPLRNYYIPYASKEEAKQELEREKTSQF
                     IDLNGTWDFQYYPSVQQLPENFFQEAFTESYDRISVPGVWQTEGFDLPQYINVSYPIP
                     FDPPYVPNDTPCGVYRRQFQIEQTDARFYLNLEGADSNCFVWINRNFVGYDQVSHSTS
                     EFDVTKYLQVGVNEIQILVMKWCDGTYFEDQDKWRMSGLFRPVYLLQRPTMHLTSYQL
                     RTLTDPKKGSGTLMVSFQRFTPIEVSLPISYELFHPDGRLIAKGECYSEQQINLDVED
                     CLFWTAETPKLYTLLLTCNGEYFKEKVGFRALTIKNRKLLLNGTPIKLYGVNYHDVNQ
                     QGTPVMTREQLRADLLLMKKLNVNTVRTAHYPKSPDFYELADEIGLYVINEADLECHG
                     VVNLYDTEANYQLIANDPTFEKPIMDRVIRSILPYQNRSSIIMWSMGNESGYGVNFEK
                     AQALARKLDDSRLIHNERSIEPVIDQVNDFSNLDVISQMYPPLEDVHRHCQRSDTEKP
                     FFMCEYSHAMGNGPGDLKTYFDLIQQYDSFLGGCVWEWTDHAINRGTLETPSYLYGGD
                     FGEKYHDGNFCVDGLLTPDRQLTVGALEYRNIHSPIQLIAVDEELQEVTLSNITAFTA
                     GSAFNLSLEWYIDGKKTVYEVMNPFILEPGKSCSLPITVPTTIHGAHTVAVSLSVYKN
                     DELYGQQQLFLKKEKMRLPRTAHHNDAPQISVKGTELIIKGESFIYIYQLAKAAFTSV
                     KINQKELLERPTQFSIWRAPTDNDRNLVNKWKAAGYAEMSLEIRKKDWKFVDDHLLIE
                     TSYRGVIPGLQPIFTMHIQLKINGAGVINQKVSVEKNPIFPDFPRFGMDWPLNDTFSK
                     VSYCGYGPYESYPDKHYAAMFGIYEMEIEEFDQPYIKPQEYGNRWQCDWVSIKNHQGS
                     TMTVYGNSNFSALPYSQAQLTEKSHHYELIKENRTWFNLSYKQNGIGSHSCGPYLEDR
                     FRFMEDTFDWNIVITFKGENR"
     gene            11874..13535
                     /locus_tag="IXT20_002058"
     CDS             11874..13535
                     /locus_tag="IXT20_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hyaluronate lyase"
                     /protein_id="HAQ4860494.1"
                     /translation="MKETKINQVKKKLSRYQEVVYRNHCLKWSKNKLNVRFNGLFYRF
                     EQLEEVWKPVTNFQSCPVQLINDPKETYDLCITHLYNQHLKNDGFYLKVTNDNQLTIE
                     SANLRGFQYAMEALLESFFSVGDQLLLPELILKHEPSVKIRGIIEGFYGIPWTHEMRL
                     SLFSYMKDNQLNTFMYAPKDDELLRKKWRELYDAQELDKFKELLSAAEASNIDFWYLI
                     SPGNDIDITCEEDIQVLLKKLEQLIELGVFQFGLLMDDIDYQLKGAAKRRFREPAFAH
                     AYLVNRVEEYLSTRLSNHELVICPTEYDNRHGSRYLATLSQEIPEQLPFFWTGPSTLA
                     ASISTEELQEMASVYQRPMLIWDNIPVNDYLEDKELLFMSPYENRTPNLSKERYQVTG
                     VVSNPMAQLEASKFTINSMANYLWNCERFDPLETWTSVVEKVVGSKLQPAYLTLTNAF
                     PNHYTSSLLSDEELLLAKDPEWVTKEMAALVQAVKWIQQEQHTSRLRTELEPWLQAIT
                     ESWTFWQEWQLKKDPKEAEEWLAKKKTHRIGRDLVTAHLEQIIKS"
     gene            13575..14507
                     /locus_tag="IXT20_002059"
     CDS             13575..14507
                     /locus_tag="IXT20_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase-like hydrolase"
                     /protein_id="HAQ4860495.1"
                     /translation="MVKRLISANYSDVKKMTSKELKQSIKASEGRVIVCETVANTLPQ
                     SGEITNAEVASAFGADLILLNGFDCYRPAVFGLPNVTIEKVFDLQQTIENPIPKLKEL
                     VGRPVGINLEPIAPKTNMMSEKVEISQGRASSVETIQKAEELGVDFICFTGNPGTGVT
                     NLEITKSVKMAKKYFSGLIIAGKMHSAGSEEPVVNKEAIISYAEAGADILLVPSVGSI
                     PGFLEQDLIEAVRLAKKYDLLTMSAIGTSQESARPETLRQLAIMNKSCGVDLQHIGDA
                     GYGGLAPAENIFETSVAIRGMRHTINCVSRSINR"
     gene            complement(14867..16360)
                     /gene="guaA"
                     /locus_tag="IXT20_002060"
     CDS             complement(14867..16360)
                     /gene="guaA"
                     /locus_tag="IXT20_002060"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HAQ4860496.1"
                     /translation="MITRRIREFGVFSELMSHRITAEEVKAMNPKGIILSGGPNSVYD
                     EKAFGIDEKIFDLGIPVLGICYGMQLITYKLGGKVKPAKNREYGKAELEITSAEAALF
                     QNTPEKQTVWMSHGDLVTQAPTDFDVVATSKDCPIASIQNAERKMYGIQFHAEVRHSE
                     YGNDLLRHFTLDVCQCTGDWTMDNFIDMEIAKIREQVGDKKVLLGLSGGVDSSVVGVL
                     LQKAIGDQLTCIFVDHVLLRKGEAEQVMESLGGKFGLNIIKVDAKERFLSKLAGVSDP
                     EEKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQGTLYTDVIESGTETAQTIKSHHNVG
                     GLPEDMQFKLIEPLNTLFKDEVRELGTQLGMPDSIVWRQPFPGPGLGIRVLGELTEEK
                     LEIVRESDAILREEIANAGLDRDIWQYFTVLPGIRSVGVMGDGRTYDYTVGIRAVTSI
                     DGMTADFARIPWEVLQKISVRIVNEVAHVNRIVYDITSKPPATVEWE"
BASE COUNT         5238 a         2689 c         3302 g         5170 t
ORIGIN      
        1 atttaaataa ctacatccat ctattctatc ttctaaaata gcctttttca tttcagttaa
       61 atagttcagc gccatttttg cactcgactt gcatagtagt tttttccata ttcctcaacc
      121 tcagcggggg ttgaagacat aaggaaaatc ctatgcgtta tgctcaactg gatctgatta
      181 aatattctaa tgtatttttc tctaccatca ttaaatggtt aatagccaaa aatcaccact
      241 tttcaatggc tccttgttgt ataagtataa ctagaagatg aacacgtctg agaaattata
      301 gaagcttttt acggtaatta ctggggctgt gtccagtagt ttttttgaag agaacagaaa
      361 aatagctcac gtgatctatt cctacttttt gactaatttc atcaatagat aagaatgtgt
      421 ctttcagtag agcacttgct cttttgattc gaaagtctag caagtaatca tgaaaggaat
      481 aacctgtttc ttttttgaag gcccgtccta aataagcggg atggtaatgg aattgtgtag
      541 ctacatcttt gagcgtaagc ttttcactat aattgttttg aacgtaatca atcacttgtt
      601 gaattgactc attaaaagaa tcatcagtta gagagttttg attagctgct gatttacgca
      661 ttttttcttc ttgtactaac ttaactaaat aaggaaaatc aatgggtttc gttatgtaac
      721 ttttaataga taaatcaatc cccttttttg catactgaaa attttggtgg gcactaatga
      781 caatactagt aaagtagcga ttggatgttt gttgcaaaga ttcgattaat tcgagtccag
      841 aaattttccc catcgtaata tctgtgataa gtaaatcaaa gcgctggttt tgcaggtttt
      901 taaatgcttc gtctccgtct gtgtagcagt gatcgataat gatgtcgggg actttcttca
      961 aaagataatg ccttatgcct tctacaattg tacaatcatc gtcaacaatt gcaattctca
     1021 acatgcatat tccccccttt ttgtattttg atagtaatcg ttgttccgac gtgtagttgg
     1081 ctttctataa caaaacgtgt atctccagga tagagatgct ttatacgtcg aaaagaattt
     1141 gagatgccga tattctcact ataaggaata tcatcattaa gtttttgttg aatatgctcc
     1201 agcttgtctt ggtccattcc tttcccattt tcaacgattt ttaacacata atggttggtt
     1261 gtttcagttg catagacagt caaacgattt tccttttttg atgaatcgaa gccgtgttta
     1321 atataatttt caacaaatgg ctgtaaagaa agttttggta acattaattg atcaatatga
     1381 tcaatcagct gatagtcaaa gttatgtcga taacgacgtt gatgtagttg caaggagagg
     1441 tcgatattaa ttaattcctc tttcaaatca attatgtcgg gctgatatgt attattgcga
     1501 tacaacttgc tcaataaata tatactgtcg ctgatttctt cgtcatcatt caatatggca
     1561 gtcattcgaa taaattccaa attattaaat agaaaatgag gctcgatttg atattgaagt
     1621 gctgcatact ttgcttcttg cttcaaccaa ttattttcat aaagtgtttt ttgatatgta
     1681 gcattatcta aataaaactg attgatatgc atagcgattt ctttcaaatc accgactttg
     1741 ttttcgatat tgattgactt gtagtctcct gtagatgaat gatttaagga agtaatgatt
     1801 tcctgatact gctggatata gttacgaaaa cttctattta ataattgatg aatagcaaga
     1861 ataaacaaac tcattaataa gaaaatcatg attgccagac ctaataaggt ttgtgacgaa
     1921 taatttggat tttcatatac ttgtaatgtg tggttaccga tagctatctg ttgcgttttt
     1981 ttccatttag aaggcgagat gtcttcagat aaattgacgg aatcaatacc attagacaaa
     2041 gaataattgt aatgtgttgg aaagagactc ttttttagcg cgttgatcga aacgtctaga
     2101 atcaatgtag catcgggcat gtcctcacta atgaaactta atgaacgaat aaaagaaatt
     2161 ttttcgtttt ttgtaggtgc gtaggcactt aagtattttt cgtaatctaa tgagatagat
     2221 atatttttaa cttcatcatc ttgcacaagt ttttttaagt tatctactaa ataggtaggg
     2281 gagcgatcct ttgacaaaat atcttccatg tattcagcag gtgatgagag gtagtagtca
     2341 ttaaggtttt cttggaaatc ataattttga tagatctctg tcacgatacc ggagagaata
     2401 cgattatatg tttccatata gtgggtgatt tgctgaatgt tttgcaggtt ttgatttttt
     2461 tctttttcaa tcacattgat ataggaaaag aacaatgtga aacaggcaag taaagtaata
     2521 aaaaaaatca atatgtatgt aaaacgcata ataacttttg ataaatcgtt tttcattcaa
     2581 agtcctcatt ttgataatct ctaattttat gaatcattat agcacatatt acaagtgatg
     2641 taaacgcttt acaaacaggg ctacttatga ttgtgaatac agtttccgtt taaaaactat
     2701 tctattgtag aggtttaata aactaaaaat aatgtataaa aaattaatgt aagcgattaa
     2761 ccaagtgggt ataatcaagg aaacgctgag agaggagaga caaaatgaag ctaaagaaag
     2821 ggttattagg aaatctaaaa aaaaaccgag cattgtttat tatgctttta ccaggtgcgt
     2881 tatggttatt tatattttgc tatataccaa tgtttggagt tgtaatcgct tttaaagatt
     2941 tcagaatttc atcaagggga ttctttacaa gtttgttcaa cagtccttgg gttggctggg
     3001 aaaatttcaa atatctcttt gcttcgcaag atgcttggat cattactcgc aatacggttg
     3061 tttataacgt tgtaggtatt ttgctaagta ctttttgtac agtgtcatta gcaatcattc
     3121 tgagccaaat ggcgaacaaa cggttattga aaaagatgca gacatttatg atttttccta
     3181 attttttatc atgggtagtc atcagttact ttgtttacgc atttttgagt acggataaag
     3241 gattaattaa tattttacta agcaattttg gtgtggaacc cataaattgg tatagtgaac
     3301 cgaagtactg gccttttatt attgttttta cgagtttatg gaaaggcgta ggctatggat
     3361 ccatcattta ttacgcgtca attatgggga tcgatactag ttattatgaa gctgctttag
     3421 ttgatggtgc cagtaaatgg caacagataa ggaatgtagt cttgccacat ttaagatcga
     3481 ttattgtcat tatgttgatt ttgtctgttg gtagtatttt tcgagctgat tttggattgt
     3541 tttatcaatt gccaagaaat tcgggggctc tttatgatgt gacgaatgta ttagatactt
     3601 atgtctaccg tggattaatg aatagcggag atatcggaat gtctacggca gcgggattat
     3661 atcagtcatc agttggcttt gtcttgattt tgattactaa taagatagtc aacaagttag
     3721 atcctagttc agcactattt taggaggaat caaaatgata tcaaaattaa aacggcagat
     3781 gtacttttcg ctgccagtcg aacggttttt cacaatatta ctaatcttat ttactcttag
     3841 ctgtattttt ccctttattt tcgtaatcgt catttcatta acagatgaaa aagcattaca
     3901 actcaacggt tacgaattat tgccggctca atggagcact gatgcatatc aatacttaat
     3961 acaagatggc ggacagttac ttcggtcatt aggcgttaca attatgatta cagtgattgg
     4021 aacgttgatt actgttttta tgacgggaac ctatgcttat gttttatcaa gagcctcttt
     4081 cccttaccgg aagttcttta ctttttatct tttttttacc atgttatttg cgggagggat
     4141 ggttccttct tacttagtga tgacgaagat gcttggctta aaaaatacaa tttgggcttt
     4201 gattctacct cttgctttta gcccatacaa tgtcattatt ttaaggacat tctttaagaa
     4261 gtcgattcca gaatcgatta tcgaatccgc taaaatggat ggctgtagtg agtttcgagt
     4321 ttttttccaa atcgttttgc ctttagcgat cccaggggtt gcaacaatag gtttattttc
     4381 aagcttaggt tattggaatg actggttcaa cgcattgctg tatattgaca ccaacaaatt
     4441 gattccattg caatatttgt taatgaagat tcaaaattca atggaattct tagcaaataa
     4501 taatgacatt acattagcgc agcaacaagc aattcaaaat agccttcctc aagaatcaac
     4561 acgaatggca atggtcgtag tagctacttt accgattgca attgtttatc cgttcttcca
     4621 aaaatacttt gttcaaggat tgacgatcgg tggagtgaaa gaataaaaaa gaataggaga
     4681 tgttaaaaga tgaaaaagaa catgcagaaa tatttgattg gtggtgtttc tgtactaacc
     4741 cttggcctat taggtgcttg tggtagcagt aatggcgaag caaataatga aacaggaacc
     4801 acaaaagtta cttggtatca ggttggagaa gaacccaaag attttgacga ggtcatgaca
     4861 aaagtcaacg aaaaattaaa ggaagaaatc aatgtagagt tggatatgcg gctgattccg
     4921 gacggggatt atcagcaaaa gcttggtgtg atgatcaatt caggagaaga atatgatatt
     4981 tgtttcgtca atggcacaga ttatgtgaat tatgggaaca aaggtgcttt cattagtctc
     5041 aacgatcatt tggataaaga gttgaaagat tatgcagctg aattgaatga aggatttatt
     5101 gaaggtgggg caattaatgg cgaaacttat ggattgccag tcaataacga tattgccaca
     5161 acggaatatt ggacctttaa taaagatttg gtagatcaat accaattgga tgtagagaat
     5221 gttgaaggtg tagaaacatt ggctccagta ttagaaaagt ttgcagaaaa aaatactgat
     5281 acgaatatta cgccaatcgg tatgacccgt accagtgtct gtattcctta tgacaatatt
     5341 atcggtggtg gtgtaccgct agcggttgca tttgaaggcg atacacaaac agttgttaat
     5401 acttatgaga ctccagaaat gatgacttat ttgaaggcta tgcatgagtt ttataaaaaa
     5461 ggatatattt tgaaagatgc agccactgtt acgacagatc catttcgaat tgatagtaaa
     5521 aactggtttt taggtcgtaa ctcaagtggt tatcaaccat tagcacaaaa aaaattagaa
     5581 gctctagcgg gacatggatt aattttaaaa ccagctggaa aagcttttaa aacgactgga
     5641 tttgcaagag tggcaatgca tgcggtatct gcttcaagta aacatccgga agcagccttg
     5701 aaattgttga attatgtgaa taccaatcaa gaagtagcgg atttaatggt ttatggaatt
     5761 gaaggaaaac actatgatcg caatgaagat ggttcgatta ctttaacaaa ctctgatgat
     5821 tattatcaag gcgcttgggc attggtaaat acttatctag tttcaaaaac ttcaccgatt
     5881 acagaagaaa gtaaaaaact agaagaagaa agtaaaaagt ttcaaaatga agcaccgatg
     5941 tctcctattt taggtttctc ttttgacgct tctgattttc gaacagaaat tgcaagtgta
     6001 caaaatatcg tgtcacaata tgaagtactt ttaagctcgg gaacagttga ccctgaacaa
     6061 tatgtaccag agttgttaga aaaactagaa aaagctggat tggataaaat tcaggcatct
     6121 gctcaagaac aatacgatga atggcgaaaa tctaacaaat aattttttta ctaaatgaaa
     6181 agaatagact aagcagttag tggggggatt caaatgaacg aaaacaaatg gaccaaagga
     6241 tttaattggg tttttcaatt gttattacta aactttactt attttatact aatttgttta
     6301 ggatttggaa ttttaggttt tttccctgct acgatagctt tgattgctgg cgttgtgaat
     6361 cgaaaaaaat atggcttgca agagtctgtg aagttcatga gtaaaatcta taagaaaaat
     6421 tttgttagat ataatctata ttgctggtca gcaactttgt tgttggccgg caatattact
     6481 acgatctggc tttgttggca gcaaagcgca ccattgtttt atttattaag tacattatta
     6541 tcggtaatta cactgttcct agtctttgca atgttgaatt gggtcgtgat caaaagtctt
     6601 gggttgtgga tgatgggaga ccgactgatt gactatttgc ttttcacagc aacgatgttg
     6661 ccaacggtag tcatgcaagg cttggtgctt ggaatttatt tccgaatatt gatgattttg
     6721 cctgtgattt ttctgtttgg cggaacagct gtaacggtaa tattgattag taaattaact
     6781 gaaagagcgt tagcaaagtt gccttgtcag atacaatcgc actaattttt tcacaaaaag
     6841 gagtatttct ttctatgaag ttaactgata aacaagtagc taatatagcc tttatccagc
     6901 aacttgagat tccattgaca caaaaagatg aagaacaaat agacttgatt tttactgaca
     6961 caaaggctaa tgtgcgacaa atcaagatta ctaaaaatga gcagcaacga atctgcttgt
     7021 cttattcaca tgaagacttg ctgtatcgag gattgtttat ctgccttcca cgttttttag
     7081 ctcaaggagg agagatgcca tacattgaag aaacctcgat cattgagacc agttattcat
     7141 tagatgcttc tcgtaatggc gtgatgaata aagagactct aaaaaaattc atttcttact
     7201 tagcgttgat ggggtacaac acattgtata tttacacaga agatacctat gaagtagaag
     7261 accttccata ttttggttac cttcgcggag cgtatacaaa agaagaagtg aatgaaattg
     7321 ttgaatttgc ttctggtttt ggcatagaag tagttccatc gatccagacc cttgctcact
     7381 tgacccaatt tttgaagtgg tatgctcaac aagaactgat ggatgatcaa aatactctac
     7441 tgatcggtga agaaaaggtt tatgcggcaa tcgaaaagat gattattgcc tgcaaagaaa
     7501 tgtatcagac agagcgtatt catttgggaa tggatgaagc ctatcaagca ggtctgggac
     7561 gatatttaag cctgtatggg tattgtgatc ggacaacttt aatgattcaa catatggacc
     7621 gtgtgttatc aattgttgat aaatataaga tgaaaccaat gatttggagt gatttcattt
     7681 ataagctatt agatgaaacc ggtaaagata ggtattacaa tcctgatttt aaactcaaag
     7741 cattagcagc taaaggatta ccagcaggat taacctatgt tcattgggac tatggttcag
     7801 atgaagtttc ctcctatgag aaagtaattg ataaccattt gaaattttgt gacattgatc
     7861 attacgtttt agctagtggt gcccatatct gggggagact agccccgaat catgggaaaa
     7921 cagaagcaat tgtttctgca gctgtaaaag cttctaaaaa taaaggtgta cgttctgtga
     7981 tgttaactac ttggggagat gaagggcagg aaacagagca ttggcattcc ttaattgggg
     8041 cattaaaatt agcagattat gtctattctg aagaagagtg gaatgatttt gaggaaatct
     8101 acgatcgttt attaggaaca ggtagttatc aattaatgtc cgaattacga tatttcgatg
     8161 aagtggatac cattacaaat aacaacttac aaatggcttc cgtgagtaaa ctgctacttt
     8221 ggcaagatcc gttagttggt atatatgact ttcatgtagc cgctcataac aaaattgcca
     8281 ctagaacaat tggtacttat tatcatgagt tagctcagaa attagatatg aataaaaatt
     8341 ggaatccaaa acaaccaatt ttaacaacta ttcgtcaacg atatatactt ttagctgaaa
     8401 ttttagcaca aaaggcagaa ctaggcatct atttacaaca ctgctgtctg gtagaagacc
     8461 ttgcaggatt aatggaatta gcgaatcggt gtgaagcact agccaatcga gtaaatgaat
     8521 tggcatctat tcatgaaata atttggtatc aaacatacaa aacctatggt tgggaagtat
     8581 tagaacaacg atatgctgca ttagctcatc gtctgaaaac cgttgcgcgt cgtattaaaa
     8641 atcatcttca ggggcaagat gccctagagg agcttttgga agaacgatta ccgttttgtc
     8701 aatatcaagt gccgcttgaa atttctggct ttaattatcg gcaaagctcg gtgtcaggct
     8761 acaattaacg aaggaatctt cccgttgaac ggaccacttg ctcatctaat gggaaagtgg
     8821 ttcgttttct attcataagt agcttaccac taactctttt attaagaaga gaaaaaaagt
     8881 aggaagtgag aaaatatgtt atcggactat tatcaaaatc tgtcagttat aagtgtcaat
     8941 acagaaccat tacgtaatta ttatattcct tatgcaagta aagaagaagc aaaacaagag
     9001 ttagaaaggg aaaaaacgag ccagtttatc gatctcaatg gtacatggga ttttcagtat
     9061 tatccttctg tccaacagtt accggaaaat ttttttcaag aggcctttac tgaaagttat
     9121 gatagaattt ctgttcctgg agtttggcaa acagagggat ttgacttacc tcagtatatc
     9181 aatgtatctt atccaattcc ttttgatccg ccttatgtac ctaatgatac cccttgtggt
     9241 gtatatcggc gtcagtttca aattgagcaa acagatgcac gcttttactt aaatctagaa
     9301 ggagcagata gtaattgttt tgtttggata aatagaaact ttgtaggcta tgaccaagtg
     9361 agtcatagta catctgaatt tgatgtgacc aagtatttac aggttggggt taatgaaatt
     9421 caaattctag tgatgaagtg gtgtgatggt acctactttg aagatcagga taagtggcga
     9481 atgagcggtc tatttcgtcc agtgtatctt cttcaacgac caactatgca tctgacaagt
     9541 tatcaactac ggacgttgac tgatcctaaa aaaggaagcg gaacattaat ggtttctttt
     9601 caacgattta ctcctattga agtatcgtta cctatttctt acgaattatt ccatcctgat
     9661 ggacgtctca tagcgaaagg ggagtgttac tcggaacaac aaataaattt ggatgttgaa
     9721 gattgccttt tttggacagc agaaacgcct aaattatata cattactttt aacatgtaat
     9781 ggtgaatatt ttaaagaaaa agttggcttt cgagcactga cgatcaagaa tcgtaagtta
     9841 ttgctgaacg gaacacctat taaattatat ggagtcaact atcatgacgt gaatcagcaa
     9901 ggcacgccag tgatgacccg agagcagtta cgtgcagatt tgttattaat gaaaaaatta
     9961 aatgtgaata ctgtacgaac ggcacattac cctaaatcac cagattttta tgaattggcg
    10021 gatgagattg gtctgtatgt aatcaatgaa gcagatttgg agtgtcatgg tgtggttaat
    10081 ctatatgata ctgaagcgaa ttatcaacta attgctaatg atccgacatt cgaaaagccg
    10141 attatggacc gagtgatccg ttctattctt ccttatcaaa atcgaagctc tatcattatg
    10201 tggtctatgg ggaatgaatc tggttatgga gtaaattttg aaaaagctca agctttagca
    10261 cggaaattgg atgattcacg gttgattcat aatgaacgct cgattgaacc agttattgac
    10321 caagtgaatg attttagtaa cttggatgtt attagtcaaa tgtatccccc actggaagat
    10381 gttcatcgtc attgtcaaag aagtgatact gaaaaacctt tttttatgtg tgaatatagc
    10441 cacgctatgg gcaacggtcc tggagatctt aaaacgtact tcgatttgat tcaacagtat
    10501 gattcctttc ttggcggttg tgtctgggag tggactgatc atgcaatcaa tagaggaact
    10561 ttagaaacgc catcttattt atacggtggt gattttggtg aaaaatatca cgacggcaat
    10621 ttttgcgtag atggtttatt gaccccagat cggcaattaa cagtaggtgc attggagtac
    10681 cgtaatattc attcgcctat tcaactgatt gctgtagatg aggagctgca agaagtaacc
    10741 ttgagcaata taactgcttt tacagccggt tcagcattta acctttctct tgaatggtat
    10801 atagatggaa aaaaaacagt ttatgaagta atgaatcctt tcatacttga gcctggcaaa
    10861 agttgttcat tgcccatcac agtacctaca acgattcatg gtgctcatac ggttgctgtt
    10921 tcgctatctg tttataaaaa tgatgagctt tatggacagc aacagctctt tcttaaaaaa
    10981 gaaaaaatga gattgccaag aactgctcat cataatgatg caccacaaat ttcagtaaaa
    11041 ggcacagagc tgataatcaa aggagaatcc tttatttata tatatcaatt agcaaaagct
    11101 gcgttcactt ctgtaaagat caatcaaaag gaacttttgg aacgtcctac tcaattttca
    11161 atttggcgtg caccaacgga taatgatcgt aacttagtaa acaaatggaa agcagctgga
    11221 tatgctgaaa tgtctttaga aatcagaaaa aaagactgga aattcgtaga tgatcatctc
    11281 ctgatcgaaa cgtcttatcg aggagttatt ccaggattac agccaatttt tacaatgcac
    11341 atacagttga aaataaatgg tgcaggcgta atcaaccaaa aagtatcagt ggagaaaaat
    11401 ccgatttttc cagatttccc tcgctttggg atggactggc cgttgaatga tactttttcg
    11461 aaggtttctt attgtggcta tgggccgtat gaaagttatc ctgataagca ctatgctgcg
    11521 atgtttggta tttatgagat ggagattgag gaatttgatc agccatatat caaaccacaa
    11581 gaatatggta accgctggca atgtgattgg gtatcaatca aaaatcatca aggatctacc
    11641 atgacggttt atggaaactc taatttttct gctctgcctt atagtcaagc tcaattaacc
    11701 gaaaagtcac accattatga attaataaaa gaaaatagaa cttggttcaa tttaagttat
    11761 aaacaaaatg gtatcggctc acacagttgc ggtccttatt tagaagatcg tttccgtttt
    11821 atggaagata cttttgactg gaatatagtg attactttca aaggagaaaa tagatgaaag
    11881 aaacaaagat caatcaagtc aaaaaaaaat tatctcgtta tcaagaagtt gtttatcgaa
    11941 accattgctt aaaatggtca aaaaataagc tgaatgttcg ttttaatgga ttattttatc
    12001 gtttcgaaca gttagaagaa gtctggaaac cggtaacgaa tttccaatca tgtcctgtac
    12061 agctcataaa tgatccaaaa gaaacatacg atttatgtat aacacacctt tataaccagc
    12121 atttgaaaaa tgacggattt tatttaaagg ttactaatga caatcaactt acgattgaat
    12181 cagcaaacct tcgagggttt caatatgcta tggaagcctt attagagagt tttttttcgg
    12241 taggagatca attattatta ccggaattga ttctgaaaca tgaaccatca gtgaaaattc
    12301 gaggaatcat tgaagggttt tatggtatcc cctggacaca tgaaatgcgc ctctctcttt
    12361 tttcatatat gaaagataac caattgaata cttttatgta tgcccctaaa gatgatgaat
    12421 tattacgtaa aaaatggcgt gaactctacg atgcgcaaga attagacaaa tttaaggagc
    12481 ttttatctgc tgctgaggct agcaatatcg atttttggta cttaattagt cctggaaatg
    12541 atattgatat tacgtgtgaa gaagacattc aagttcttct gaaaaagtta gaacaactga
    12601 ttgagcttgg cgtatttcaa tttggtctat taatggatga tattgactat caattaaaag
    12661 gtgctgctaa acgccgtttt cgagagccag cattcgctca tgcctattta gtgaatcgag
    12721 tagaggaata tcttagcact cggttgtcca atcatgaatt agtcatttgt cctacagaat
    12781 atgataatcg tcatggttct cgctatttag caacgttaag tcaagaaata ccagaacaat
    12841 taccgttctt ttggactggc ccaagcacgt tagcggcgtc aatttctaca gaagagctgc
    12901 aggaaatggc ttctgtctat caacggccga tgctgatttg ggataatatt ccggtaaatg
    12961 attatttaga ggataaagaa ctattgttca tgagtccata tgagaatcga acaccgaatc
    13021 tttctaagga gagataccag gttacaggag tagtttcgaa tccaatggct caattagaag
    13081 cttcaaagtt tacgatcaat agtatggcta attacttatg gaactgtgag cgctttgatc
    13141 ctttggaaac ttggacgtca gtcgttgaaa aagtagttgg aagtaaacta caaccagctt
    13201 atcttacctt aaccaatgct tttccaaatc attacacaag cagtctgctt tcagatgaag
    13261 aattactgct agccaaagac ccagaatggg tcacaaaaga aatggctgct ttagtccaag
    13321 cagtaaaatg gatccagcaa gaacaacata catctagatt gagaacagaa ttggagcctt
    13381 ggttacaagc aatcacagaa agttggactt tctggcaaga atggcaatta aaaaaagatc
    13441 caaaagaagc agaagaatgg ttggctaaga aaaaaacaca ccgcatagga cgtgatttag
    13501 taactgccca tttagagcaa attatcaaaa gttagatatc ttaaaaaata aaaagcaaga
    13561 aaaaggagaa caaaatggtc aagagattaa ttagtgcaaa ctacagtgat gtcaagaaaa
    13621 tgacgtcaaa agagttgaag caatccatca aagcgagtga aggacgggtg attgtttgtg
    13681 aaacggttgc taatactctt ccacaatctg gagaaattac gaatgcagaa gttgcgtcag
    13741 catttggtgc agatttgatt cttttaaatg gtttcgattg ttatcgtcct gcagtttttg
    13801 gactgccaaa tgtgacgatt gagaaggttt ttgatctaca gcaaacgatt gaaaacccga
    13861 ttcctaaatt aaaagaacta gtcggtcgtc ctgtgggaat taatttagaa cccattgccc
    13921 ctaaaacaaa tatgatgagt gagaaagtag aaatcagcca aggaagagca agctctgttg
    13981 aaacgataca aaaagcagaa gaactaggag tcgattttat ttgctttacc ggtaatccag
    14041 gcactggagt aaccaattta gaaattacta aatccgtaaa aatggcaaaa aagtattttt
    14101 cgggattgat tattgctggg aaaatgcata gcgccggctc agaagagcca gttgtgaata
    14161 aagaggcgat tatttcttat gcggaagcag gcgcagatat tttactagtt ccttcagtag
    14221 ggtctatccc aggcttttta gagcaagacc taattgaagc ggttcggttg gcaaaaaaat
    14281 atgatttgtt aacgatgtct gcaatcggta ctagtcagga aagtgcccga ccagaaacat
    14341 tgcggcaatt agccattatg aataaaagtt gtggtgttga tttacaacat attggagatg
    14401 ctggatacgg gggattggca ccagcagaaa atattttcga aacctcggtt gcaattagag
    14461 gaatgcgaca taccatcaac tgcgttagcc gctcgatcaa tcgctaacaa aattaaataa
    14521 aaaatggtta aattgattat ttaagtggga acatacttga gatatcagct aacttctgtc
    14581 attttaaaaa aagggactgt gacagaagtg aaaatagaca aaaaatatcc gaacaagaga
    14641 ctaggatagc agaaaaacta tcgttttgca acgaggacca actggaacgt aggagcaatt
    14701 ctcttattgt tcggatattt cgcttgttca aggacttatg tctgtagtgc ccttttttgg
    14761 taggaaaaat ggtagggaac catatcattt ttaatgaaaa tcaagcattt caaaagttga
    14821 aaaacgttga tttatcggca ttccccatcc atattatatt tacgtcttat tcccactcca
    14881 ctgttgcagg tggtttgctg gtaatatcat acacgatacg gttaacgtgt gcgacttcat
    14941 ttacgatacg gacactgatt ttttgcaaga cttcccaagg gatacgggca aagtcagcgg
    15001 tcatgccgtc aatagaagtg acagcacgga tacctacagt gtagtcatac gtacggccat
    15061 ctcccataac cccaacagag cgaatgcctg gtaggacggt gaagtattgc cagatgtcgc
    15121 gatctaaacc ggcattggcg atttcttcgc gcaagatagc gtctgattca cggacgattt
    15181 ctaatttttc ttctgtcaat tcaccaagga cacgaatacc taagcctggt ccagggaatg
    15241 gttggcgcca aacgatgctg tcaggcatgc ctaattgggt acctaattca cgtacttcgt
    15301 ctttgaacaa tgtgttcaat ggctcgatca gcttgaattg catgtcttca ggtaatccac
    15361 ctacattgtg atgtgatttg atggtttggg cagtttcagt tcctgattcg atcacatcgg
    15421 tataaagggt tccttgtgca aggaaagaaa cgccttcttc accggcaagt ttggttgctt
    15481 catcatcgaa gacatataca aattcatttc caatgatttt acgtttttct tctggatcag
    15541 acacgccagc aagttttgat aagaaacgtt ctttggcatc gacttttata atgttcaagc
    15601 caaattttcc gccaagactt tccataactt gttctgcttc acctttacgc aacaatacgt
    15661 gatcaacgaa gatacatgtt aattgatcgc cgatcgcttt ttgtaaaaga acaccaacaa
    15721 cagaggaatc tactccgcct gaaaggccta ataaaacttt tttgtcaccg acttgttcac
    15781 gaatttttgc gatctccatg tcgatgaagt tatccattgt ccagtcacca gtacattggc
    15841 agacatctag tgtaaaatgg cgcaacaagt catttccata ttcggaatgg cgaacttctg
    15901 catggaattg gattccgtac attttgcgtt ctgcattttg gatcgatgcg atggggcaat
    15961 ctttgctggt agcaacgaca tcgaaatcag tcggtgcttg ggtaactagg tcaccatgac
    16021 tcatccaaac ggtttgtttt tcaggagtgt tttggaaaag ggccgcttct gcagaagtga
    16081 tttccaattc tgcttttccg tattcacggt ttttggctgg tttaactttt ccgcctaatt
    16141 tataagtaat caactgcata ccgtaacaga ttcctaaaac tgggatcccc aaatcaaaaa
    16201 tcttttcatc aataccaaag gccttctcat cataaacact gtttggtccg ccagaaagga
    16261 tgattccttt agggttcatt gcctttactt cttctgcggt aatgcggtgg ctcatcaact
    16321 ctgagaaaac accgaactca cgaatacggc gagtgatcag ttggttatac tgactaccgt
    16381 aatcaagtac gataatttt
//
LOCUS       DACGLE010000061        16334 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0534
            SAMEA3304449-rid9560453.denovo.065, whole genome shotgun sequence.
ACCESSION   DACGLE010000061 DACGLE010000000
VERSION     DACGLE010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304449
            Sequence Read Archive: ERR985064
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16334)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16334)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702225.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702225.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 20:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,045
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 27 of 67
            Pseudo Genes (incomplete)         :: 25 of 67
            Pseudo Genes (internal stop)      :: 19 of 67
            Pseudo Genes (multiple problems)  :: 4 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16334
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304449-rid9560453.denovo.065"
                     /strain="VREN0534"
                     /db_xref="taxon:1352"
     gene            complement(119..775)
                     /locus_tag="IXT20_002061"
     CDS             complement(119..775)
                     /locus_tag="IXT20_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HAQ4860497.1"
                     /translation="MDSRVLKEYLDKNDFPIIRKGYHKYLTFEGMEDSHTYILKEGTV
                     KASVISNDGREFNLRYIKDLEIVSLLKDEYSQFIDSPYNIRVESEYAEFYRINRVRFW
                     QNINQDPKLQHYVKEYYRYRLLYSMKKMQQMLLNGKLGAVCTQLYELYDLFGVRTEEG
                     MLIDFSITNEEIAKFCGISTASSVNRILKQLKKDGIITVKDHHIVILNMDLLEDNIIF
                     "
     gene            968..1246
                     /locus_tag="IXT20_002062"
     CDS             968..1246
                     /locus_tag="IXT20_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4860498.1"
                     /translation="MNYWMNTIINRLETAYQTRFDMKASLVFLNDAYQNSIELIKAVD
                     ENPTNECEEFLNLFMSTRDLFIRQLVDRYPSNYHDVEVQIQKLKAYSA"
     gene            1265..2473
                     /locus_tag="IXT20_002063"
     CDS             1265..2473
                     /locus_tag="IXT20_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ4860499.1"
                     /translation="MEKKLFNKYFIGITLINFIVYLIYYLLMVIMAVIAQDTLHASLS
                     QAGFASGIYIIGTLLARLFMGKQLELFGRKFTLRGGAIFYLLSTLAYLYTPTIGALYL
                     VRFLNGFGYGTVSTATNAIVTAYIPASKKGEGINYYGLSTSLAAAVGPFLGMILLNMT
                     DFRFIIWFSIVLVFFVTIACLVFPVKNIPLSEEQKISLRSWNINSFIEKKALFITGIA
                     FLMGLSYSSVLSFLSSYTKVIHLVAAGSFFFVVYALVITFTRPLTGRIFDVKGEQYVM
                     YPSYLFLTAGLFLLSVTTNSVTLLISGALVGLGYGTFMSNGQAVCLKIVREHRISIAL
                     STYFIGLDLGLGVGPYLMGMLKSLTSFRGLYVIAGVIPLICTALYLVNGRKHSHEAEG
                     ELIESLKNEI"
     gene            complement(2621..2986)
                     /locus_tag="IXT20_002064"
     CDS             complement(2621..2986)
                     /locus_tag="IXT20_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2512 family protein"
                     /protein_id="HAQ4860500.1"
                     /translation="MKHVKALVVKAIMIWAILWVVLTGLYGVSFMDSTIVGVIIVVMI
                     YVLGDLLILRKVGNIAATIADAGSAAVILWLYLYFMNDTVDIWMKVLIPALLIGVAEW
                     FFHKWLLSQGIVPDERKME"
     gene            complement(3133..3906)
                     /locus_tag="IXT20_002065"
     CDS             complement(3133..3906)
                     /locus_tag="IXT20_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAQ4860501.1"
                     /translation="MLNEVENRQQEIVNYLKNNQFARVTDLIELVNYSEATIKRDLVF
                     LEKSGLIRRVRGGAMLIDNRKIDVPYLMKITKLEEETEKRKIADTASSLIRDDMVLFL
                     DSSTTSLHLVRNLARFDGLQIITNGIVTAAMLSEYTTAKVSVLGGAIVPKRATINGSK
                     AYNDALSYYADLAIMSCRGLDFSQGVTETHEGEAMVKKAFRKQSDRLAVLVTQQKIAH
                     KFMHQAIAFHEIDYLVTSKELTKEQYAKVKEHHIECLYG"
     gene            complement(4080..4940)
                     /locus_tag="IXT20_002066"
     CDS             complement(4080..4940)
                     /locus_tag="IXT20_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HAQ4860502.1"
                     /translation="MLYTMKELLKVAKENQFAVPAFNICSYDMMKAIIEEAERLEAPV
                     ILEIHPDEIAYLSDEFVAAVRAYAYTSKLPIVIHLDHGGTIEDVLRAIRNGYSSVMID
                     ASLKSFDDNVQITKKVVALAHAVNVSVEAELGTIGNNGSAEGGAETIIYTDPDQAKIF
                     VKETGIDTLAVAIGTAHGLYPKNRTPKLNIDLLKELNQQIDLPFVLHGGSGNPDAEVS
                     EAVKYGVAKVNLSSDLKSVFFNALREILSENPSMYEPNAIYPAANKKVQEVVKHKMEI
                     LNTVGKASLY"
     gene            complement(4940..6358)
                     /locus_tag="IXT20_002067"
     CDS             complement(4940..6358)
                     /locus_tag="IXT20_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ4860503.1"
                     /translation="MKIVGVTACPTGIAHTYMSAEKLEVTARNLNHQAKFETQGVKTE
                     NQLSEQEIKEADAIILAVDKEIELDRFAGKKVKRVSTSRAIKEPQVVIDEALRDIGVF
                     VVSNEKEPAANEKKPTIYNHFMNGVNYMLPFVIAGGIIIALSFAFGITAADPNSADYN
                     VLAAAFSRIGGDTAFAMMVPALGAGIATSIAGKAGFAPGIVAGLLASTGGSGFLGGMI
                     GGVLAGYICDFLANKISVKKEFSAIYQLIVVPFLSILIIGLLMVFVIEQPITWVLDAL
                     TNWLNSLGNTSGLLFGLLIGIMMAADMGGPINKSISTFSIGLMSAGVNAPIAACMAAG
                     MTPPLGIALATLLFKHKFTKEERTSGQSCWILGASYITEGAIPFAVSDPIRVIPSLMT
                     GSAVAAGISMAVGVTSMAPHGGIWVMLIPNVMNNLPMYVLAIASGTVTTAFMLGLLKK
                     TVKEKKQDKWTKNENLTNEVEA"
     gene            complement(6415..6864)
                     /locus_tag="IXT20_002068"
     CDS             complement(6415..6864)
                     /locus_tag="IXT20_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAQ4860504.1"
                     /translation="MRFDQLIAEELIVFDPSIDSKGSLFERIGDVLKKTNKVRNVQKV
                     VKALFKREEEISTGIEDGFGIPHAKSKYVTKPALCFFHTAKLTDYSGIDEMPIEYIFA
                     IVVPEKSADMHLEILSTLSRRLMNADFRKGIKEATTPADIIRQISQE"
     gene            complement(7309..7572)
                     /locus_tag="IXT20_002069"
     CDS             complement(7309..7572)
                     /locus_tag="IXT20_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HAQ4860505.1"
                     /translation="MKENIHPDYHPVVFMDSTTGFKFLSGSTKTSQETVEWEDGNTYP
                     VIRVEVTSDSHPFYTGRQKFTQADGRVDRFNKKYGLKDANAAE"
     gene            complement(7671..8966)
                     /locus_tag="IXT20_002070"
     CDS             complement(7671..8966)
                     /locus_tag="IXT20_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HAQ4860506.1"
                     /translation="MSDYLTMAELENKTLKEVYSYAKEFKIPYYSKMNKKELSLAVIR
                     AQAEKQGFYYMEGILDIVGQDGYGFLRPINYGPSAEDIYISASQIRRFSLRNGDKVAG
                     KARPPKESERYYGLMHVESVNGKDPEEAKERPHFPALTPLYPQRQLQLETTPQRLSTR
                     MIDIFSPIGFGQRGLIVAPPKAGKTSVLKEIANGITENYPEVELIVLLIDERPEEVTD
                     LERSVKGDVVSSTFDLQPQNHTRVSELVLERAMRLVEDKRDVVILMDSITRLARAYNL
                     VVPPSGRTLSGGIDPAALYKPKKFFGAARNIEEGGSLTILATALVDTGSRMDDVIYEE
                     FKGTGNLELQLSRELSERRIFPAIDIKKSGTRKEELLMPSEELEEIWKLRKNMTGDSL
                     EYTEQLIRFLRKTKNNQQFFKEFQDVSFGKKNPTRNLKR"
     gene            complement(8963..10234)
                     /locus_tag="IXT20_002071"
     CDS             complement(8963..10234)
                     /locus_tag="IXT20_002071"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HAQ4860507.1"
                     /translation="MKKIVIKGNRPLAGEVTISGAKNSAVALIPAAILADSPVILEGV
                     PDIQDVHSLIEILEIMGATVHFSNNILEIDPREVVSIPMPHGKINSLRASYYFMGTLL
                     GKFGEAVVGLPGGCYLGPRPIDLHVKGFEALGAEVTNEHGAMYLRTENKGLRGNRIFM
                     DVVSVGATINVMLAAVKAKGQTVIENAAREPEIIDVATLLNNMGTKVRGAGTDVIRIE
                     GVETLHGCRHFMIPDRIEAGTYLALAAAVGNGIKVKNVIFEHLESFIAKLQEIGVHMK
                     ISEDEIEVYPSKELKPANIMTYPYPGFATDLQQPLTALLLMTTGTSEIIDTIYAKRVN
                     HVPELARMGADITVEGNMIIVNGPNKLHGTEVVASDLRAGACLVIAGLLAEGTTTIYN
                     VDYILRGYDHIIEKLTALGASIEMIEEVGAE"
     gene            complement(10399..11268)
                     /locus_tag="IXT20_002072"
     CDS             complement(10399..11268)
                     /locus_tag="IXT20_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphate aldolase"
                     /protein_id="HAQ4860508.1"
                     /translation="MPVVSGAEFLKAARKGGYAVGGFNTNNLEWTQAILEAAEAKQAP
                     VLIQTSMGAAKYMGGYKICKDLITDLVEAMNITVPVAIHLDHGDYEAALECIELGYTS
                     VMFDGSHLPFEENLKLAKDVVEKAHAKGISVECEVGSIGGEEDGIIGNGELADIEECK
                     QMVATGIDYLACGIGNIHGQYPENWKGLAFDHLQAIADAVGPDMPLVLHGGSGIPQDQ
                     IEKAISMGISKVNVNTEFQLSFAAATRKYIEAGKDKEGKGFDPRKLLAPGKAAIVEDA
                     KSHIDWFGSANKA"
     gene            complement(11560..13170)
                     /locus_tag="IXT20_002073"
     CDS             complement(11560..13170)
                     /locus_tag="IXT20_002073"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HAQ4860509.1"
                     /translation="MTKYIFVTGGVVSSIGKGIVAASLGRLLKNRGLKVTIQKFDPYI
                     NVDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFIDINLNKYSNVTTGKIYSEVLRKE
                     RKGEYLGATVQVIPHITNEIKEKIMRAAKMTDSDVIITEVGGTVGDIESLPFLEALRQ
                     MKADVGTDNVMYIHTTLIPYLKAAGEMKTKPTQHSVKELRGLGIQPNILVVRTEQPVS
                     QSVKNKLAQFCDVEPEAVIESPDVDTLYSIPLVLQAQGMDQIVCDHLKLDTPEADMTE
                     WKELEERVLNLKKKVRIALVGKYVELPDAYISVVEALKHSGFAYNSDIEIDWIKAQEL
                     TRENVEERLKDADGILVPGGFGDRGVEGKIEAIRYARENDVPFLGICLGMQMACVEFA
                     RNVVGLEDASSAETNPDTANNIIDLMADQENIENLGGTLRLGLYPCKIKKGTKTAEAY
                     DGADVVQERHRHRYEFNNKYRQLFEEQGLVFSGVSPDNRLVEIVELPEKKFFVGCQFH
                     PELISRPNRPQKLIKAFVGASLADHETK"
     gene            complement(14363..15703)
                     /gene="glnA"
                     /locus_tag="IXT20_002074"
     CDS             complement(14363..15703)
                     /gene="glnA"
                     /locus_tag="IXT20_002074"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HAQ4860510.1"
                     /translation="MVKKQITGEEIKRIIEEENVRFLRLMFTDILGTIKNVEVPVSQI
                     DKVLENKMMFDGSSIEGFVRIEESDMYLYPDLSTWMIFPWESAHGKVARLICDIYNPD
                     GTPFAGDPRGNLKRALKDMRDLGFTSFNLGPEPEFFLFKLDEDGGITTTLNDKGGYFD
                     FAPTDLGENCRRDIVLELESLGFEVEASHHEVAPGQHEIDFKYADVVDACDNIQTFKL
                     VVKTIARKHGLHATFMPKPLFGINGSGMHCNMSLFNDEGNVFYDKDGELELSETAYHF
                     LGGLLKHARAYTAVCNPTVNSYKRLVPGYEAPVYVAWSGRNRSPLVRVPESRGLSTRL
                     ELRSVDPSANPYLAMAVLLQAGLDGIRNQITPPAAVDRNIYVMDEEEREAAHIQDLPS
                     TIHNAIKELRKDNVMIDALGQHIFSNFVEAKRLEWAAFRQTVSEWEREQYLELY"
     gene            complement(15731..16123)
                     /locus_tag="IXT20_002075"
     CDS             complement(15731..16123)
                     /locus_tag="IXT20_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAQ4860511.1"
                     /translation="MGEKELRRSMSVFPIGTVMKLTDLSARQIRYYEEQDLVHPERSE
                     GNRRMYSLNDIDMLLEIKDYLSEGLNMAGIKRVYEMAQAKKQEAKNKKPLTDHDVRQI
                     FRDEILAQGGLSKTGHYQSQGLQRQFFD"
BASE COUNT         4661 a         3460 c         2776 g         5437 t
ORIGIN      
        1 tgttcaatca gctgaccctc ggtattagct caaaaaacgg caaaatatga ggaactattt
       61 ttccgtttgt ttctctaata ctcaggtcaa aacgctgatt tcacaacctc tttgttcatc
      121 aaaagataat attgtcttct aacaagtcca tattcaaaat aacgatatga tggtctttaa
      181 cggtgataat accatctttt ttcagttgtt ttaaaattct gtttacactg ctagcagttg
      241 agatgccaca aaatttggcg atttcttcat tcgtgatcga aaaatcgatc agcatccctt
      301 cttctgtccg cacaccgaat aaatcatata attcgtaaag ttgcgtacaa acagctccca
      361 gctttccgtt taatagcatt tgctgcattt ttttcatcga ataaagcaga cgatagcggt
      421 aatactcttt tacatagtgt tgtagctttg ggtcttggtt gatattttgc caaaagcgca
      481 cacgattgat tcgataaaac tctgcatatt ctgattcaac tcgaatatta tacggcgaat
      541 cgatgaattg tgaatattcg tcttttaaaa gagagacgat ttcaagatct ttgatatagc
      601 gaaggttaaa ttctctccca tcatttgaaa taacacttgc ttttaccgtt ccctctttca
      661 agatatacgt atgggaatct tccatccctt cgaatgtcaa atatttatga tatcctttgc
      721 gtataatcgg aaaatcattt ttatccaaat attcctttaa gactctacta tccaatgatg
      781 ctcccccatc caaactttca tctaaaaaga tcatacagta ctttaaaact ttttaaagtc
      841 caaaggtatt ttactaatga tattaacatt tgttaagtac ctttttccat cacttatgcg
      901 atttttttcc ttttttttat tcgttatact gcagaagtaa aattttttaa gaatagaggg
      961 aaaaaacatg aattactgga tgaacacaat cattaatcga ttagaaacag catatcaaac
     1021 aagatttgat atgaaagctt ctttagtctt tttaaatgat gcgtatcaaa actcgatcga
     1081 attgatcaaa gcagtggatg agaatccaac aaacgaatgc gaagaatttt taaatctgtt
     1141 tatgtctaca agagatttat ttattcgcca acttgttgat cgctatccat ccaattatca
     1201 cgatgtagaa gttcaaatac aaaaattaaa agcatattca gcttaagggg aatgtataac
     1261 atacatggaa aagaaattat ttaataaata ttttattgga atcacattga tcaattttat
     1321 tgtctacctg atttattatc tgttgatggt catcatggca gtcattgcgc aagacacctt
     1381 acacgccagc ttgagccaag caggttttgc ttctgggatc tatatcatcg gaacattgct
     1441 ggcacgctta ttcatgggga aacagttaga actatttggt cggaagttta ccttaagagg
     1501 cggcgcgatc ttctatctcc tttcaacatt agcatatctt tatacgccaa ctatcggggc
     1561 attgtattta gttcgattct tgaatggatt cggctacggt acagtttcta cagctacaaa
     1621 tgctatcgtt actgcatata tccctgcatc taaaaaaggg gaaggcatca attattatgg
     1681 attaagtact agtttagctg ccgctgttgg gccattttta ggaatgattt tgctgaatat
     1741 gactgatttt cgtttcatta tctggttttc aattgtactg gtcttctttg ttacgattgc
     1801 ctgccttgtc tttcctgtaa aaaatattcc attatctgaa gaacaaaaaa tatctcttcg
     1861 ctcatggaat atcaatagtt ttattgaaaa gaaagcactt ttcattacag gaattgcctt
     1921 tttgatgggt ctttcttatt caagtgtttt atcattttta tcttcttata caaaagtaat
     1981 ccacttagtt gctgctgggt catttttctt cgtcgtatat gctttggtca tcacatttac
     2041 acgtccattg actggaagga ttttcgatgt caaaggagaa caatacgtga tgtatcctag
     2101 ctatttattc ttaacagcag gattattttt actgagtgta acgacgaata gtgtgacatt
     2161 gcttatctct ggagcattag tcggcctagg ttatggtacg tttatgtcaa atggacaggc
     2221 tgtttgttta aaaatcgtcc gcgaacatcg aatcagtatt gctttatcta cctactttat
     2281 tggtttagac ctaggcttag gcgttggtcc ttatttgatg gggatgctaa aatctcttac
     2341 ttcatttaga ggattgtatg tgattgccgg agtcattcca ttgatatgta cagcactcta
     2401 tcttgttaat ggccgaaaac atagtcatga agcagagggc gaattgattg aatcattgaa
     2461 aaatgaaatt tgatcttgat gaatagataa aaaaaaccgt tcagatctga aaataatctg
     2521 agcggttttt tgagtaaaaa tagtttatga ttattcttaa ttaaacatct atttattttt
     2581 aaagctttca tttatcgata agaatagttt aactgttgat ttattccatt ttccgttcat
     2641 ctggtacgat gccttgactt agcaaccact tgtggaagaa ccattctgcc acaccgatca
     2701 atagagcagg aatcaatact ttcatccaaa tatcgactgt atcattcata aaatatagat
     2761 agagccacaa gataactgca gctgaaccag catctgcgat cgtcgctgcg atgttcccta
     2821 ctttacgtaa gatcaacaga tcccctagca catagatcat gactacaata attaccccaa
     2881 caatcgttga atccataaag ctaacaccgt ataaacctgt cagcacgacc cacagaatcg
     2941 cccagatcat gattgccttc actaccaacg ccttcacatg tttcattttc tgcactcctt
     3001 tctcctggat aaaaaagtaa gattatctaa caagataacc ttttcattat tataccatcg
     3061 gctgagagaa gcgagcaaag ataaggtcct atctaaaaat gaggaagcca cttagaacat
     3121 tcacgtaata atttatccgt acagacattc gatatggtgt tcctttactt tcgcatactg
     3181 ttcttttgtc agttctttac ttgtcaccag ataatcgatt tcatgaaaag caatggcttg
     3241 atgcatgaat ttatgtgcaa ttttttgctg tgtaactaaa acggcaagcc ggtctgattg
     3301 ttttctaaaa gcttttttga ccattgcctc tccttcatga gtttctgtta ctccttggct
     3361 gaaatctagc ccgcgacaac tcattatcgc taaatccgca taatatgaca atgcatcgtt
     3421 ataggctttt gaaccgttga ttgttgctcg tttcggaaca attgcccctc ccaatacaga
     3481 tacttttgcc gttgtatatt cactcaacat agcagctgtg acgatgccat tcgtaatgat
     3541 ctgcaaacca tcaaatcttg ctaggtttct gactaagtgt aaagaggttg tactggaatc
     3601 caagaataag accatatcat ctcgaatcaa agaagaagct gtatctgcaa tcttacgttt
     3661 ttctgtttct tcttctaatt tggtgatctt catcaggtaa ggcacgtcaa tttttcgatt
     3721 gtcaataagc atggcgccac cgcgaactct acggatcaat ccactttttt ccagaaaaac
     3781 aaggtcacgt ttaatcgtgg cttcgctata atttactaac tcgatcaaat cggtgactct
     3841 tgcaaattgg ttgtttttta gataattcac aatctcctgc tgtctattct ccacttcatt
     3901 caacataaaa tcaccccatt tactttatat agtaacagga aaaaagaggt tgtgacagaa
     3961 gtgattggct tcgagaaata agaagaaatt tttcgaaaaa actacttctg aacatcgtgt
     4021 gtggttaaga gggtatgaca agactattgt cataccctct tgaccacaca tttttatttt
     4081 tagtataaac tagccttgcc tactgtgttc aaaatttcca ttttatgttt gactacttct
     4141 tgtacctttt tattagctgc tggataaatt gcattcggct cgtacatcga tggattttca
     4201 ctcaatattt ctctcaaagc attgaaaaag acgcttttaa gatctgagct caaattcact
     4261 ttggcaactc cgtattttac tgcttcgctg acttctgcgt ccggattacc tgaaccgcca
     4321 tgtaaaacaa atggaagatc gatttgttgg ttcaattctt tcagtaagtc gatatttaat
     4381 tttggagttc tgtttttcgg atacaagcca tgcgctgttc caattgctac agcaagtgta
     4441 tcgattcctg tttctttcac aaaaatcttc gcttgatcag gatctgtata aatgattgtt
     4501 tcagcacctc cttcagcaga accattattc ccaatcgttc caagctcagc ctccactgag
     4561 acatttaccg catgagctaa cgcaaccact tttttcgtaa tctgtacatt gtcgtcgaat
     4621 gatttcaaag acgcatcgat catcacggaa gaatagccgt ttcgaatcgc ccgtaagaca
     4681 tcttctatcg taccgccatg atccaagtgg ataacgatcg gcagtttact tgtataagca
     4741 taggctctta ctgccgcaac aaactcatca cttaaatagg cgatttcatc aggatgaatt
     4801 tctaagatta ctggtgcttc taatctctct gcttcttcga tgattgcttt catcatatca
     4861 tagctgcaaa tattgaaggc tggtactgca aattgattct ctttagccac tttcaataac
     4921 tctttcattg tatataacat tacgcttcca cctcatttgt tagattttca tttttcgtcc
     4981 acttgtcttg ttttttctct ttcactgttt ttttcaatag tccaagcata aaggctgtag
     5041 tgactgtacc tgatgcaatt gccaagacat acataggtaa attgttcata acatttggaa
     5101 ttagcatcac ccaaatccct ccatgaggtg ccattgaagt aactcctact gccatggaaa
     5161 tccctgcagc gactgctgaa cctgtcatca aactaggtat cacgcggatc gggtcagata
     5221 ccgcaaacgg tatagcacct tctgtaatat acgaagcgcc taaaatccag catgattgac
     5281 ctgaagtccg ttcttctttg gtaaatttat gtttgaataa taaagtggct aaagcaatac
     5341 ctaggggagg tgtcattcct gcagccatac aagcagcaat cggtgcattt actccagctg
     5401 acatcaaacc gatcgagaaa gtcgaaatcg atttattgat cggcccaccc atatcagctg
     5461 ccatcatgat cccgataagt aatccgaaca atagcccgga tgtatttccc aagctattca
     5521 gccaatttgt caaagcatcc aacacccatg tgatcggttg ttcaatcaca aataccatca
     5581 aaagcccaat aattaagatc gataaaaagg ggacgacaat cagctgataa atagcagaaa
     5641 attctttttt tacgctaatt ttatttgcca gaaaatcaca gatataccct gctagcacgc
     5701 caccaatcat tccgccaaga aaacctgaac cacctgtaga ggccagtaat ccggcaacaa
     5761 ttcctggggc aaaacctgct tttccagcaa tcgatgtagc aatccccgcg cctaacgcag
     5821 gaaccatcat ggcaaacgct gtatctcctc cgatccttga aaatgcagct gctaacacat
     5881 tatagtctgc tgagttggga tcagccgctg taatcccaaa ggcaaaactc agtgcgatga
     5941 tgattccgcc tgcgatcaca aatggaagca tgtaattcac accattcatg aaatgattat
     6001 agattgtcgg ctttttctca tttgcagctg gttctttttc attagacaca acgaatacgc
     6061 caatatctct caatgcttcg tctattacta cttgcggttc tttgattgct cggcttgtag
     6121 agacacgctt tacttttttc ccggcaaatc ggtccaattc gatttcttta tctactgcaa
     6181 gaatgattgc atctgcctct ttgatttctt gttcagataa ctgattttct gtttttacac
     6241 cttgtgtttc gaattttgct tgatggttta gatttcttgc tgttacttct aatttttctg
     6301 ccgacatata agtatgagca attccagtcg ggcaagcagt tacaccaacg attttcatga
     6361 ttattttcct cctttttctt cactatcgat actcggacaa agactctact attttcattc
     6421 ttgactaatc tgtctgatga tatctgctgg cgtagtggct tcttttattc cctttctaaa
     6481 atctgcattc atcaatcgtc tggagagtgt gctgagaatc tctagatgca tatctgctga
     6541 tttttcagga actacgatgg caaaaatata ttcaattggc atttcatcta tcccgctata
     6601 gtccgttaat ttggctgtat gaaaaaaaca cagtgctggt tttgtcacat atttgctttt
     6661 tgcatgggga atcccaaatc catcttctat tcctgtggag atctcctcct ctcgtttgaa
     6721 tagtgcctta acgacttttt gaacatttct taccttattt gtttttttca acacatcccc
     6781 tattcgctca aataatgatc cttttgaatc tatcgatgga tcgaaaacaa tcagttcttc
     6841 tgcgatcaat tgatcaaacc tcataaaaaa cctctccccc acaaaaaatg ttaacgtttt
     6901 cttggaactg aggtcagtat agcatcgcta gctgtaccgt tcaatattat gaatgaacag
     6961 aggaaaaaga cccatcatcc gcgtgtataa gtcattaaaa tgaacgatct agctcacaaa
     7021 caatcgcaag tctataacaa aacaaaaagc cttccttgaa aggaaagctt agcttctaat
     7081 tttaggtaaa tggtttttta cttcgaaata acccctcttc atttcgttaa tataaggtgt
     7141 ttttatcaag tggataatta aaactatata ctagtttttc attgttttgt gcattatgat
     7201 cctacgactt tttcagcatc aaacgaagtt ttaaattggc aaaaaacata aaaaaacaga
     7261 ctatccctat cagatagtct gctaaacgtt gagttatcag cgtttttatt attccgcagc
     7321 gtttgcgtct ttgagaccgt attttttgtt gaaacggtcc acacgtccgt ctgcttgtgt
     7381 gaatttttga cgtccagtgt agaatggatg tgagtcagaa gttacttcga cacggatgac
     7441 tgggtatgta ttaccgtctt cccattcaac tgtttcttgt gaagtttttg ttgaacctga
     7501 caagaatttg aaaccagttg ttgaatccat gaatactact gggtggtaat ctggatggat
     7561 gttttctttc atgatttttt cgctcctttg ccctgctcca tttgctgaac cagagttgat
     7621 ttgtcgattt acaacattaa catcttaaca tgtttcagtc aaggttgcaa ttatcttttc
     7681 agatttctgg tcggattttt tttaccaaag gagacatctt gaaattcttt gaaaaattgc
     7741 tgattgtttt ttgttttacg caaaaagcgg atcagttgtt ccgtatattc taatgaatct
     7801 ccagtcatat tttttcgaag tttccaaatt tcttctaatt cttcacttgg catcaataat
     7861 tcttcttttc gggtacctga ttttttgata tcgatggctg ggaagatccg acgttcagat
     7921 aattcacgtg aaagttgtaa ttctagattt ccagttcctt tgaattcttc atagatcacg
     7981 tcatccatac ggcttcctgt atcgactaaa gcggtcgcta aaatcgtcaa actgccacct
     8041 tcttcgatgt ttcttgctgc cccgaaaaat ttcttaggtt tatacaatgc ggctgggtca
     8101 atcccgccac tcaatgtacg tccgcttggt ggaacgacta agttataggc ccgagcaagt
     8161 ctagtaatac tatccatcaa gataacgaca tctcgtttat cttcgactaa acgcattgcg
     8221 cgttccaata ctaattcaga aactcgagta tggttttgcg gctgcagatc aaatgtagaa
     8281 gataccacgt cgcctttcac gcttcgttct agatccgtta cttcttccgg tcgttcatca
     8341 atcagtagaa cgattaattc tacttccgga tagttttcgg tgataccatt tgcgatttct
     8401 tttaatacag aagttttccc tgcttttggc ggtgcgacga tcaatccacg ctgaccaaaa
     8461 ccgattggag aaaagatatc gatcattcgg gtagatagtc tttgcggtgt tgtttccagt
     8521 tgaagctgtc tttgcggata aagaggtgtt aaggctggaa aatgcggacg ttcttttgct
     8581 tcttccggat cttttccatt aacgctttcc acgtgcatca aaccataata acgttccgat
     8641 tctttaggtg gacgggcttt tcctgccact ttgtccccat ttctcaaact gaatcgtctg
     8701 atttgtgagg cagaaatata gatatcttct gcacttggtc cataattgat tggccgtaaa
     8761 aaaccgtaac catcttgacc aacgatatcc aatattcctt ccatataata aaatccttgt
     8821 ttctctgctt gtgcacgaat cacagctaga gataattcct ttttattcat cttactgtaa
     8881 taaggaattt taaattcttt ggcataacta taaacttctt ttaacgtttt gttctctagt
     8941 tctgccattg tcaaataatc gctcattcag ctcccacttc ctctatcatt tcaattgatg
     9001 cccctaaggc tgttagtttc tcgataatat gatcataacc gcgtaagata taatctacat
     9061 tgtaaatcgt cgttgtccct tctgccaata gtccagcaat cactaaacaa gcacctgccc
     9121 gaagatcaga agccacgact tctgtgccgt gaagtttatt tggaccattc acgatgatca
     9181 tatttccttc cacggtaata tcagcgccca ttcgtgctag ttccggcaca tggttcacac
     9241 gtttcgcata gatcgtatcg atgatttctg aagtgcctgt agtcatcaat agtaacgcag
     9301 taagtggctg ctgcagatct gtggcaaatc ctggataagg ataggtcatg atgtttgcgg
     9361 gttttaattc tttcgagggg taaacttcga tttcatcttc gctgattttc atatggacac
     9421 caatttcctg caattttgct ataaagcttt ccaaatgttc aaagatcaca tttttcactt
     9481 tgataccatt tcctactgca gctgctaatg ctagataggt tcctgcttcg atgcggtcag
     9541 gaatcatgaa atgccgacag ccatgtaatg tttcgactcc ttcgatacgg atcacatctg
     9601 tacccgctcc tcgaactttt gtccccatat tattcagtaa agttgccaca tctatgattt
     9661 ccggttctcg tgcagcgttt tcaattactg tctgtccttt tgcttttaca gcagctaaca
     9721 tcacattgat cgttgctccg actgaaacca cgtccatgaa aatccgattg ccacgcaatc
     9781 ctttattttc tgtccgtaaa tacatggcac catgttcatt tgtcacttca gcacctagag
     9841 cttcaaatcc tttgacgtgt aggtcaatcg gtctagggcc taaataacag ccacctggca
     9901 ggcctactac cgcttcgcca aatttaccaa gcagtgttcc cataaaataa taagaagcac
     9961 gcaaactatt gatttttccg tgcggcatcg gaatcgatac gacttctcga ggatcgattt
    10021 ctagtatgtt gttggaaaaa tggacagttg cgcccatgat ctctaaaatt tcaattaatg
    10081 aatgaacatc ttgtatgtcc ggcacacctt ccaagatcac tggggaatca gctaagattg
    10141 cagctggaat caatgcgact gcactatttt ttgcaccgct gatcgttact tcaccagcta
    10201 aaggtcggtt tcctttgatt acgatttttt tcatctgttt ccaccttgtt tattaatctt
    10261 ttttcatgga tatgcttgta ttttatcata gataaaaaaa agaaacgagc atggtctgcc
    10321 gttttaagta aaaaagaatg gagagcgact tcaaaagtcg ttctccattc ctatatatcg
    10381 tatctgttat gtggatgctt acgctttgtt agctgaaccg aaccagtcga tatgagattt
    10441 agcatcttca acgattgctg ctttacctgg agctaataat ttacgagggt cgaatccttt
    10501 accttcttta tctttaccag cttcgatata tttacgagta gctgcagcaa atgataattg
    10561 gaattcagtg ttaacattta cttttgagat acccattgag atagcttttt cgatttgatc
    10621 ttgaggaata cctgatccgc catgtaatac taaaggcatg tctggaccta cagcatcagc
    10681 aattgcttgt aagtggtcaa aagctaatcc tttccagttt tctggatatt gaccatggat
    10741 gttaccgata ccgcaagcta agtagtcgat acctgtagca accatttgtt tgcattcttc
    10801 aatatcagct aattcgccgt taccgatgat tccgtcttct tctccgccga ttgaaccaac
    10861 ttcacattca actgagatac cttttgcatg tgctttttca acaacgtctt tagctaattt
    10921 caagttttct tcaaatggta gatgtgaacc atcgaacatt actgaagtat agcctaattc
    10981 gatacattcc aaagcagctt cgtaatcacc atggtctaag tggatagcaa ctggaacagt
    11041 gatgttcatt gcttcaacta aatcagtgat taaatcttta cagattttgt aaccacccat
    11101 gtatttagct gcacccattg atgtttggat aagtactggt gcttgttttg cttcagccgc
    11161 ttcaaggatt gcttgagtcc attccaagtt gtttgtgttg aatccgccga cagcgtaacc
    11221 gcctttacga gcagctttta aaaattcagc tcctgatact actggcataa taaaattcct
    11281 cctaagtttt gtaaaactaa atttgttaag acaattctta accaacgacc ctattttacc
    11341 aaacatttcg gtagttttct actaaaatca atgaaaatga aaattttcaa gaagtttttt
    11401 gcaaaaaata aaatatccaa tagcgcttta atcgaaaaaa gtcttttgct ttctatcttt
    11461 tttcctacaa ataataacaa atcagaaaaa gagaaaagtg acaagaaaat tgcttatccc
    11521 cttctcttgc tacttttctc tttctatcaa gttcaaaaat tattttgttt cgtgatctgc
    11581 taatgatgct ccgacaaatg ctttgattaa tttttgcggg cgatttggac gagaaatcaa
    11641 ttctgggtgg aattggcatc ccacaaagaa ttttttctct ggcagctcta cgatttctac
    11701 taaacggtta tctggtgaga cacctgaaaa gactagccct tgttcttcaa acaattgacg
    11761 atatttgtta ttgaactcat agcggtgacg gtgacgttct tgcacaacat cggctccatc
    11821 atatgcttca gccgtcttcg ttcctttttt gattttacat gggtataatc caaggcgtaa
    11881 agttccaccc aaattctcga tgttttcctg atcagccatt aaatcaataa tattgtttgc
    11941 tgtatctgga ttcgtttcag ctgatgacgc atcttctaat ccaacgacgt tacgtgcaaa
    12001 ttcgacacaa gccatctgca taccaagaca aatacctaaa aacggaacgt cattttcgcg
    12061 agcataacga atcgcttcga ttttcccttc tactccgcga tcaccaaatc caccaggaac
    12121 caagattcca tctgcgtctt tcaaacgttc ttctacattt tctctcgtta attcttgtgc
    12181 tttgatccaa tcgatctcga tgtctgaatt ataggcgaaa ccagaatgtt tcaatgcttc
    12241 aactactgag atatatgcat ctggtaactc gacatattta ccgacaagcg caatccgtac
    12301 ttttttcttc aagttcaaga cacgttcttc taattctttc cattctgtca tgtctgcttc
    12361 tggtgtatct agttttaaat ggtcgcaaac aatttgatcc attccttgtg cttgtaaaac
    12421 taatgggatg gagtaaagtg tatctacatc tggcgactcg ataactgctt ctggttcgac
    12481 atcacaaaat tgtgccaatt tgtttttcac gctttgagaa acaggttgct ctgtacgtac
    12541 caccaaaata ttcggttgga tacctaaacc acgtagctct tttacactgt gctgcgttgg
    12601 tttagttttc atttctccag cagctttcaa gtaaggaatc aatgttgtat ggatatacat
    12661 aacattatct gtaccgacat ctgctttcat ttgacgaagt gcttcaagga atggcaaaga
    12721 ttctatatcc cctactgttc cccctacttc tgtaatgatc acatctgaat cagtcatttt
    12781 agctgctcgc atgatttttt ctttgatctc atttgtaata tgaggaataa cttgaacagt
    12841 tgctcccaag tattcgccct tacgttcttt acgcaaaact tcagaataga tttttcctgt
    12901 cgtcacgttg gagtatttgt tcaaattgat gtcaataaaa cgttcgtagt ggcccagatc
    12961 caaatctgtt tcagcaccat catctgttac gaatacttct ccgtgctgat atgggctcat
    13021 cgtccccgga tccacgttga tgtatggatc aaatttttgg atagttactt ttaagccgcg
    13081 gtttttcaat aagcgtccta aagaagctgc aacaatccct tttccgattg atgaaacgac
    13141 accgccggtc acaaaaatat attttgtcat aagttcaaaa actccttttt aatttatgtg
    13201 caggaaaagt ctcagacaat aaacaaagct ccctattctt caataagaat agggagccga
    13261 aatttctgta aacttctgac cctctaaaag ggtgcccaag gtgtataata caagctgact
    13321 aagggaaagt caagtactca aatgaatact atccgcttac aattttttct ttttcgatat
    13381 cctttgagaa attatttctt aagtccttta tgagtgcaaa aaaaatttaa cccacataat
    13441 aagcttgata taaacatagc taacgttaaa cctaagcaaa tcagaaaatc gttactgaaa
    13501 aattttaagt gaaataatag ataaaaaaat aaaacaaaca ttatgataga taatatccca
    13561 cctaacaaag aactactttt tcctacttgc agcaacagtt tatatagctt atcgtagaaa
    13621 caatcttttt ctttcaccct tatttacctc ctttacgtaa aaaacgtagt ccacagttct
    13681 cggatttttg gcatatcttt tctattaaat aatatcaaaa acaaaacaat tccactaatt
    13741 ttttcaaaga tgaccttttt ttcaacaaaa gtgccttgta gggattcatt gaaggtaacg
    13801 aatcacataa gtaaaaaata aggagatgcg acaaaagtcg tgcctcctta tttctaaata
    13861 aacggtgttc tcctgccaac tcctcggtca caagtcacaa tatccatcaa acatgagaag
    13921 ctgtttttga aattatttct tgtgattcgg agttaaatgg taggctcaca acctctggat
    13981 aaacggtatt caaaagccag tttctcggta ttaagccaaa tttatgcaaa aatgtagaaa
    14041 gccattttca caaaattttt cttaatactc gaaactaaac gtctttttca aaacctcttg
    14101 gtaaacggtg ttcaaaagct tgcatctgcg gtaataagtt cagccaattc aaaaacaaga
    14161 aaagctgttt ttgcttggct attcttatta cttgttgcag ggcagctttt tcacaacctc
    14221 tccactaaac ggtgttcaat cagctgaccc tcggtattag ctcaaaaaac ggcaaaatat
    14281 gaggaactat ttttccgtta gtttctctaa tactcaggtc aaaacgctga tttcacagcc
    14341 tctaattcac ggtattcctg ttttaataaa gttctagata ttgttctctt tcccactcgg
    14401 aaacagtttg tctgaatgcc gcccattcga gacgttttgc ttcaacaaaa tttgagaaga
    14461 tatgttgtcc taatgcgtca atcattacat tatccttacg caattctttg atagcattat
    14521 ggatagttga aggtaagtcc tggatatgtg cagcttctct ttcttcctca tccattacat
    14581 aaatgttacg gtcaacagct gctggcggtg tgatctgatt acggataccg tctagtcctg
    14641 cttgcaacaa tactgccatt gccaagtatg ggtttgcaga tggatcaact gaccgcaatt
    14701 ccaaacgtgt ggataaaccg cgagattctg gtacacgtac taatggcgaa cgattacggc
    14761 cactccatgc gacataaaca ggcgcttcat aacctggaac gagacgttta taagaattga
    14821 ctgttggatt gcaaacggct gtgtaggcac gagcatgttt cagcaaacca cctaagaaat
    14881 gataagctgt ttcacttagt tccaattcac cgtctttatc atagaaaaca tttccttcat
    14941 cattgaacaa agacatattg caatgcatac cagaaccatt gatcccaaat aacggtttcg
    15001 gcatgaaagt cgcgtgaagt ccatgcttgc gtgcaatcgt tttgacaacg agcttgaagg
    15061 tttggatatt atcacaagca tctaccacat ctgcatattt aaagtcaatt tcatgctgtc
    15121 ctggcgctac ttcatgatgt gaagcttcca cttcaaagcc tagactttct aattccaaca
    15181 cgatgtcacg gcggcaattt tcacctaaat ctgttggtgc aaaatcaaaa taaccacctt
    15241 tatcattcaa ggtcgttgtg atcccaccgt cttcgtctaa tttaaataag aagaattctg
    15301 gttcaggtcc taaattgaaa gaggtaaaac caaggtcgcg catatctttc aatgctcgtt
    15361 tcaaattccc gcgaggatcg ccggcaaatg gtgtcccatc cggattatag atatcacaaa
    15421 ttaaacgagc aacttttcca tgagcacttt cccacgggaa gatcatccaa gtggagagat
    15481 ctggatacaa atacatatcg ctttcttcaa ttcgaacaaa tccttcaatc gaagaaccgt
    15541 caaacatcat tttattttct aatactttgt cgatctggct cactggaact tctacatttt
    15601 tgatcgtacc taagatgtct gtgaacatca atcgtaaaaa tcgtacgttt tcttcctcaa
    15661 tgattcgttt gatttcttca cctgttatct gtttctttac catggtatcc ctcttccttt
    15721 tttaatttat tcagtcaaag aattgtcttt gcaatccttg cgactgataa tgacctgttt
    15781 tgcttaaccc accttgcgcc aaaatctcat cacgaaaaat ttgtcgaacg tcatgatctg
    15841 tcaacggctt cttgtttttt gcttcttgtt ttttcgcctg tgccatttcg tatacacgtt
    15901 tgatcccagc catattcaat ccctctgaaa gatagtcttt aatctcaagc aacatatcaa
    15961 tatcattcag cgaatacata cgtcggtttc cttcgcttcg ttccggatga actaaatctt
    16021 gttcttcata atagcgaatt tgccgagcag acaaatcagt cagcttcata actgtaccaa
    16081 ttggaaatac ggacatagat cggcgcaatt ccttttctcc catcagacgt cccctttcgt
    16141 tttataagtt ggctaaagtg taccaattaa aaaaaatatt gtcaactgca tatgttaggt
    16201 tttctaacat gagatctcta ttttttataa aataacaaaa aaataagaac acaaaaaagg
    16261 tatctagttg attttcgtac cagatacctt ttatttttta ttatactgta ctatccttta
    16321 ttagtgtttt tatt
//
LOCUS       DACGLE010000062        15974 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0534
            SAMEA3304449-rid9560453.denovo.066, whole genome shotgun sequence.
ACCESSION   DACGLE010000062 DACGLE010000000
VERSION     DACGLE010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304449
            Sequence Read Archive: ERR985064
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15974)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15974)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702225.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702225.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 20:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,045
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 27 of 67
            Pseudo Genes (incomplete)         :: 25 of 67
            Pseudo Genes (internal stop)      :: 19 of 67
            Pseudo Genes (multiple problems)  :: 4 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15974
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304449-rid9560453.denovo.066"
                     /strain="VREN0534"
                     /db_xref="taxon:1352"
     gene            complement(180..1547)
                     /gene="nhaC"
                     /locus_tag="IXT20_002076"
     CDS             complement(180..1547)
                     /gene="nhaC"
                     /locus_tag="IXT20_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HAQ4860512.1"
                     /translation="MNKNISLKESIAVLIVLLALLGVLIIGFQLSPHIPILAAFMCLL
                     FYGRFKKFTWDEIHDGIIKGITPGIIPILIFLLIGVLVASWILSGTIPTIMVYGFKLV
                     SVRFFLPTAFLVCAIVGVTVGSSFTTISTIGIAFLGIGHLLGFNDAMTTGAVVSGAFL
                     GNNCSPLSDTTNLAAGIGGVNLFEHIAGMRYTDFPAFVLSLIFYTILGHNSHSADLAS
                     INEMIQNMKASFWISPWTLIPLVILFGGAIKKIPAIPTLLAGSTAALVLSFIHEKSLT
                     VGKAADILMNGYVANTPDPKLDELLSRGGIMSMLGSASLILLALALGGLLIKYLIVDT
                     IVQELKEKMDRPSRLVGFTALSCIGINLIVGEQYLSIILPGETFKRSFDDVGLSKTYL
                     TRTLADSGATVNSLVPWGVSGTFIMGTMKVSALHYLPFAFFSILAPVFTILGGFLLNR
                     RRVKN"
     gene            complement(1656..3077)
                     /locus_tag="IXT20_002077"
     CDS             complement(1656..3077)
                     /locus_tag="IXT20_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ4860513.1"
                     /translation="MSKSEVSKMGLGTFIGLTMALCATVRSIPTLAAVGWTLIFYSIF
                     AVLFFAGPISMVSGELSTMLPQEGGPQLWVKTALGSKWGFVVAWLLWVQMFPGMVMVA
                     STLGPLLGNTFGNVELGNNHWFVLGCILVIYWIITILNLKFDMAKVGGNIGVWLGVYI
                     PVVIMFVLGVLAAFKVGLVSNGYLGDFSWSKAFPDLEHIDSLKYLAGITFIFVGIEMS
                     SVYMPRLKDATKNYTKGVFIALIGLVLLNVINAMLVANVVPDGKMELANITQPILIDC
                     QILGLPEVIGNIFSFMVFIGVLLQLSAWVTGPSKTIIQVAREGFLPPKFGFHKENKYG
                     VSRNVVLTQSIVISLFALLYGVMDDVSAVFLTLTNATTVIYCIVYILIAVSLLKMRKK
                     HPEFERPYRIGKNGNGLAWVVSCMLIFSIIVVVFATLGTATLSDALLVAAITVVMFVI
                     PLIINHFKKDSWGIEVEKSLEEK"
     gene            complement(3320..5197)
                     /gene="tdc"
                     /locus_tag="IXT20_002078"
     CDS             complement(3320..5197)
                     /gene="tdc"
                     /locus_tag="IXT20_002078"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HAQ4860514.1"
                     /translation="MSESLSKDLNLNALFIGDKAENGQIYKALLNELVDEHLGWRQNY
                     MPQDMPIITPEEKSSASFEHTVNKTKDVLSEISARMRTHSVPWHNAGRYWGHMNSETL
                     MPSLLAYNFAMLWNGNNVAYESSPATSQMEEEVGMEFAKLMSYKDGWGHIVADGSLAN
                     LEGLWYARNIKSLPLAMKEVTPELVAGKSDWELMNLSTEEIMNLLDSVPEKIDEIKAH
                     SARSGKHLEKLGKWLVPQTKHYSWLKAADIIGIGLDQVIPVPVDHNYRMDINELEKIV
                     RGLAAEKTPILGVVGVVGSTEEGAIDGIDKIVALRRVLEKDGIYFYLHVDAAYGGYGR
                     AIFLDEDNNFIPFEDLKDVHYKYNVFTENKDYILEEVHSAYKAIEEAESVTIDPHKMG
                     YVPYSAGGIVIKDIRMRDVISYFATYVFEKGADIPALLGAYILEGSKAGATAASVWAA
                     HHVLPLNVTGYGKLMGASIEGAHRFYNFLNDLSFKVGDKEIEVHPLTYPDFNMVDYVF
                     KEKGNDDLVAMNKLNHDVYDYSSYVKGSIYGNEFLTSHTDFAIPDYGNSPLQFVNQLG
                     FSDEEWNRAGKVTVLRASVMTPYMNKEEHFEEYAEKIKAALQEKLEKIYADQLLASEA
                     K"
     gene            complement(5488..6744)
                     /locus_tag="IXT20_002079"
     CDS             complement(5488..6744)
                     /locus_tag="IXT20_002079"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HAQ4860515.1"
                     /translation="MNIIDELTWRDAINQQTNEERLRELVEEKSISLYCGVDPTGDSM
                     HIGHLIPFMMMKRFQLAGHHPYILIGGGTGTIGDPSGRKTERVLQTMEQVQHNVDALS
                     NQMRKLFGKDANITFVNNYDWLSKISLLEFLRDYGKNFNINTMLAKDIVASRLEVGIS
                     FTEFTYQILQSIDFLHLHKTYDVQLQIGGADQWGNITAGLDLIRKLEGPEAEAFGLTI
                     PLMLKADGTKFGKTAGGAVWLDPKKTSPFEFYQFWLNQDDRDVVKYLKFFTFLSQEEI
                     EDLAKKVETEPEKREAQRRLAEEVTRFVHSEEDLKEAQKITQALFSGNIKELNAEEIA
                     QGFGKMPNVEISSTPENIVELLVSTKIEPSKRQAREDVSNGAISINGDRVTDLNFVIN
                     PSDEFDGKFVVIRKGKKNYFLAKVID"
     gene            7324..8625
                     /locus_tag="IXT20_002080"
     CDS             7324..8625
                     /locus_tag="IXT20_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HAQ4860516.1"
                     /translation="MEKKQKIILGATSALTIVILILSLFFVIRSSTKKEPIIPEKKIT
                     EKAKEPTDDKQQLVTQAKNLAQSYDYDQAIALLEKDSKNGSTETQQLLQTLKKEKEQL
                     IDWSDPTQISHVFFHSLIVDPQKAFHSQQAQGYKDYMVTVEEFNRSIEQLYQNDFVLV
                     NLNDLIQKDEQGNLSFNGLALPEGKKPLVLSQDDVSYYEYMDNSGFPSRLVVSNNNEI
                     KNVYKEKDKEEIGDFDMVPLIDAFVKKHPDFSYRGAKGTLALTGYNGVLGYRTSKSQY
                     GDNEKTKKEIKEAKKVAEQLKKDGWSFASHTWGHLNMTQASLEDVKKDNELWQQEVAP
                     ILGKTNILIYPFGADISDWQPYSAENQKFAYLKSQGFDIFCNVDASTPAWGQLGTDFY
                     RNARINIDGIRFQSDLDGKNPILDPFINVHDVYDKEARESA"
     gene            complement(8694..9293)
                     /locus_tag="IXT20_002081"
     CDS             complement(8694..9293)
                     /locus_tag="IXT20_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAQ4860517.1"
                     /translation="MNGFEKRAQEKKKEILQAAFDLMNTDIGTAGVTMDKVAENAQVS
                     KATIFKYFGSKEGLIYEVFFAFMNDLGQEAKALIAENLPFEETLIAMSENKIRYLNRV
                     NKQFYIDLMTYYTKKEKKEFSDAMDAYTQESFSIMLDLFHRGRKEGKVDLKYSDEFLI
                     LYFQAMVEGISKPEIYAKLLPYTAEWTEVLIKGIAPKKK"
     gene            9547..10428
                     /locus_tag="IXT20_002082"
     CDS             9547..10428
                     /locus_tag="IXT20_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ4860518.1"
                     /translation="MAEIVKVTHLKKSFGRFEALKDVSFTVHSGEVVGFIGPNGSGKS
                     TTIRTLLGIIKKDGGTATIFGQDVWEDSLSIHERLSYVPGDISLWGNLTGGAIIDLFM
                     KLHGKGSAQKKDELIQRFSLDPKKKAKNYSKGNRQKVGLIAALAVDSDLYLFDEPTSG
                     LDPLMETIFQEEVEKLKEQGKSILLSSHILSEVERLADKVVIIREGTLDEEGTLDELR
                     HLTRSTITIETEAENSHLAQLNGVHDFVQKGHLAQFAADNEAFDVILAEASKLGVKKF
                     ESIPPTLEDLFMRHYEG"
     gene            10441..12051
                     /locus_tag="IXT20_002083"
     CDS             10441..12051
                     /locus_tag="IXT20_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetronasin resistance protein"
                     /protein_id="HAQ4860519.1"
                     /translation="MTDKFSRWSTLFSQNLSRDWKKILIWLVGVTAFSGGFVPAFEEL
                     TSGQGLAGLYETLQNPAMIAMVGPTPASNAAEYTLGAMYANEMLLFCSLFAAVISVLH
                     VVSQTRKKEELGLIELIRSFRVGRQANSLAVIAESILINLLLAISISCTMIFFQADSI
                     TAEGAWLFGLSVGSMGILGSAVALFAAQLMPSSASSNGLSLAFLGGLYLLRAITDVAD
                     SSLSMWNPLGWSYLTYPFTTNNWIPLLYLFLFSLILIISSFVLEEHRDLNTGYIPERE
                     GKPYAKRSLLSLRGLLFYLNKGMTISWLITFGLMGAAYGSIYGNMQQFLESNDLMKQM
                     FIQSGVSIEKNFTSTIMVVLICLAAILPIASINRLFTEETHGRMNQLFATKISRNQLY
                     WTNMLLAILFAILAILISSGALGLSALASMKDAKDMDLLMFLKAGFNFLPAVLVFIGI
                     SGFLFGWLPRIGKLAYIYLAYSFALNYFGGILDLPEWFSKTAVPSWVPRMPNDSFDLS
                     VFLTLSCISLALFVLGSIGYRRRDLLEG"
     gene            complement(12128..12787)
                     /locus_tag="IXT20_002084"
     CDS             complement(12128..12787)
                     /locus_tag="IXT20_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HAQ4860520.1"
                     /translation="MSHDHLCVSFVPLFNHLVQQDQEKVHALVKHHYVEKGEQILAPN
                     GEPQLVIVASGSMKVYQLSASGKEQLLRVIEPGGYEGESQLLGVPNESLFGEALEKTE
                     ICILRQKDFSELLLHYPALSLKLLAINVEKLTKVQQQTTFLMMEKVEERVAVYLLDLA
                     KATGKDHFFLPMKMKELAAFIGTTPETLSRKFKLLEENGYVKRKNREVWIIDKEQLED
                     L"
     gene            complement(13070..13735)
                     /locus_tag="IXT20_002085"
     CDS             complement(13070..13735)
                     /locus_tag="IXT20_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4860521.1"
                     /translation="MELGKSDYQLFDRPIYAFKQLKESHPTDKIEQIKKEYKEHWQKW
                     KEIQLQTAALLPDMYGMSKPKIESWTNGWNLRSHFWSAYRSESRQDENACLAVLLNQK
                     QYQIYLMYQHYKSEERYGSIAAYNQLLAILKEWSKTVDIKDYYIWPQPEHELDDHLAL
                     SDYLSDTKKQEELKKAMRDRTFQMGKLFFYPQEIEHVEQITAETLQELAPLYQKLGAE
                     KFQ"
     gene            complement(13882..14487)
                     /gene="dhaL"
                     /locus_tag="IXT20_002086"
     CDS             complement(13882..14487)
                     /gene="dhaL"
                     /locus_tag="IXT20_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit L"
                     /protein_id="HAQ4860522.1"
                     /translation="MKLTVQEIQEWLTQFAEAINQNKQYLSDLDTPIGDGDHGNNMGR
                     GVSAYEEAFQADHPETISDTFKVFSMAMISKVGGASGPLYGSAFMNMMKATKEVDSID
                     SQEKLGEVIEQGTAGIQSRGKAEADDKTMLDVWLPVTEALKAGNLTKEIIDQAKEHTK
                     VLVAKKGRASYLGERAIGHIDPGAASSAILFSTLLDVINQP"
     gene            complement(14515..15498)
                     /gene="dhaK"
                     /locus_tag="IXT20_002087"
     CDS             complement(14515..15498)
                     /gene="dhaK"
                     /locus_tag="IXT20_002087"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HAQ4860523.1"
                     /translation="MKKIMNDPSNIVEEMLEGLVKSYPELVHRVESSRVVAKNQKAEQ
                     VGLVSGGGSGHEPSHAGFVGEGMLSAAVLGDVFTSPTPDQIQTAIKEADSGKGVLLIV
                     KNYTGDALNFDMAKELAAMDDIEVESVIVDDDIAVENSTYTAGKRGVAGTVLVHKIVG
                     DAARNGASLAELKELGEKVVQATKTIGLALRAATVPEVGKPGFELGEDEIEYGVGIHG
                     EPGYRREKMQPSKVLAKELVTKILDDYSNLPKEAGVLVNGMGGTPLMEQFVFMNDVLA
                     LLEERGVNVVFRKVGNFMTSLDMQGLSLTLIDLTETQWKDSLESNVQTISW"
     gene            complement(15500..15895)
                     /gene="dhaM"
                     /locus_tag="IXT20_002088"
     CDS             complement(15500..15895)
                     /gene="dhaM"
                     /locus_tag="IXT20_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS-dependent dihydroxyacetone kinase
                     phosphotransferase subunit DhaM"
                     /protein_id="HAQ4860524.1"
                     /translation="MKKSILLISHSQKVTDGIKEMIEQMQKSEEVQIFSLGGINGEIG
                     SDPMKIVEAVNESSDAASYFVFADIGSAVLNSELAKDMLEEEQQQRYHLVDAPLVEGA
                     FAAAITAGITDDTQQILTEAQNAGKKGWE"
BASE COUNT         4581 a         3337 c         2901 g         5155 t
ORIGIN      
        1 gtgggacaaa agtaaagaac acttttgtct cacgctctaa acacgtataa acggcggaag
       61 cagaagcaac tccttcggaa ataagctgaa attcacaaaa atttaaaaag caattttctt
      121 gagtttcttc ttatttcttg gagttaaacg cttctgtccc agcctctttt ttccttcctt
      181 caatttttta cacgtcgacg attcaataaa aaaccgccta atattgtaaa gacaggtgca
      241 agaatcgaga aaaaggcgaa tggcaagtaa tgcaacgcgc taactttcat cgttcccata
      301 atgaatgtgc cgctgactcc ccacggtacg agagaattga cggtcgcacc tgaatccgct
      361 agtgttcgtg tcaaataggt ttttgataag ccaacgtcat caaaagaacg cttgaatgtt
      421 tctcctggaa gaatgatgga cagatactgt tctccaacga tcagattgat tccgatacag
      481 ctcaaggcag taaacccgac taaacgagaa ggtcggtcca tcttttcttt taattcttga
      541 acgatggtat caacgatcag atatttgatc agtaacccac ctaatgccaa tgcaagtaaa
      601 atcaaactag cagaacctaa catactcatg atcccgcctc gagaaagcag ttcatctagc
      661 tttggatcag gtgtatttgc gacataacca ttcatcaaga tgtctgctgc ttttccgacc
      721 gtcaaacttt tctcatggat aaaagaaaga acgagagcag ctgtcgatcc cgctagtaat
      781 gtagggatag ccggaatttt tttgattgcc cccccaaata aaataactag tggaatcaat
      841 gtccaaggag agatccaaaa gctcgctttc atgttttgga tcatttcatt gatactagct
      901 agatcggctg aatgactgtt atgtcctaat atcgtataaa aaatcaacga aaggacgaat
      961 gctgggaaat ccgtataacg cataccagcg atatgttcga atagattaac tcctccaata
     1021 cccgctgcca agttggtcgt atctgataaa ggcgagcagt tattccctaa aaaggcaccg
     1081 gaaacgactg cgcctgtagt cattgcatca ttgaatccaa gtaaatgacc gatccccaaa
     1141 aaagcaattc caatcgtcga aatcgttgta aatgaactgc cgacagtcac tccgacaatt
     1201 gcacaaacaa gaaatgctgt cggtaaaaag aaacgcacag aaacaagttt aaatccataa
     1261 accataatcg tagggatggt tccagataaa atccatgaag caactaagac accaatcaaa
     1321 agaaaaatca ggattggaat gatccctgga gtgattcctt tgatgatccc gtcatggatc
     1381 tcatcccatg tgaacttttt aaatctgcca taaaacaaca aacacatgaa agctgcaagg
     1441 atcgggatat gtggcgagag ctggaatccg atgatcagta ctcctaggag tgccaacaac
     1501 acgatcaata cagcgatact ttcttttaaa gaaatatttt tattcatcgt cgatctccta
     1561 tttcaaatga aaaggccctg ccatgaaaaa actattcact tggcaggacc tcctactaaa
     1621 tcagcgacaa aacgtcggct gaaagactta tttttttatt tttcttctaa acttttttct
     1681 acctcaatcc cccaagaatc tttcttgaag tgattgatga tcaatggaat aacgaacatg
     1741 accactgtga tagctgctac taataatgca tcactcaatg tcgctgtacc taatgtagca
     1801 aatacgacaa cgatgataga gaagatcagc atgcagctga ctacccaagc taatccgttt
     1861 ccgtttttac caattcggta aggacgttcg aattctggat gtttcttacg cattttaagc
     1921 aatgaaacag caatcaaaat gtacacgata caataaataa cagttgtagc atttgtaagt
     1981 gtcaagaata cagcacttac gtcatccatt acaccataaa gcaaagcaaa taatgaaata
     2041 acgattgatt gtgttagaac gacatttcta gatacgccgt atttgttttc tttatggaat
     2101 ccgaattttg gtggaaggaa tccttcacga gctacttgga tgattgtttt tgatggtcca
     2161 gtaacccaag ctgataattg taaaagcaca ccaataaata ccatgaagct gaagatattt
     2221 ccgatcactt ctggtaagcc taggatttga cagtcaatca agataggctg cgtgatattt
     2281 gctaattcca tttttccatc tggtacgaca tttgctacaa gcatcgcatt gatgacattc
     2341 aataaaacta atccgatcaa agcgataaat acgccttttg tataattttt tgttgcgtct
     2401 ttcaaacgag gcatgtaaac agaactcatt tcgatcccaa caaaaataaa tgtgatacca
     2461 gctaagtatt tcaatgaatc aatatgttcc aagtcaggga aagcttttga ccatgaaaag
     2521 tcacctagat agccgtttga gactagtccg actttgaagg ctgccaaaac acctaaaacg
     2581 aacatgatga ctactgggat atatacccct agccatacac cgatgttccc gccgactttt
     2641 gccatatcga atttcaagtt caagatcgtg atgatccagt agataactaa aatacaacct
     2701 aaaacgaacc agtgattatt gccaagttct acattaccaa atgtgtttcc taacaatggt
     2761 cctaaagtag aagcgaccat taccatacct gggaacattt gcacccataa tagccatgca
     2821 acgacaaatc cccatttgct gcccagtgct gtttttaccc ataactgagg accgccttct
     2881 tgaggaagca tcgtggaaag ctcaccggaa accattgata tcggaccggc aaaaaacaaa
     2941 actgcaaaaa ttgaataaaa aatcaatgtc caaccgactg cggctaacgt aggaatactc
     3001 cgaaccgttg cacacagtgc cattgtcaga ccgataaagg tcccaagacc cattttactt
     3061 acttcagatt tactcaccgt tgacactctc cttgattttt tggataaaat aacggtgtct
     3121 ttgaagtagt ttcagcaata aacgaaacaa ttcagcgaat atttgagggg attctttgag
     3181 tttattgctt gaagggtttc ttcaaagaca acctcaaaac aaagggatcg aagagcttag
     3241 gtctcccctt gctcttcaga tccaaacatg cgtcagaaac agttagtatt caagctgttt
     3301 tctaacatga ttaccttgct tattttgctt cgcttgccaa taattggtct gcgtagattt
     3361 tttccaattt ttcttgaaga gctgctttga ttttttcagc atattcttca aagtgttctt
     3421 ctttgttcat gtatggtgtc attacgctgg cacgcaatac agttactttt ccggcacggt
     3481 tccattcttc atctgagaat cctagttggt tcacaaattg taatgggctg ttgccatagt
     3541 ctgggatagc aaagtcagta tgtgaagtta agaactcatt accgtaaatg cttcctttca
     3601 cgtatgaaga ataatcatat acgtcatggt tcaatttatt cattgcaact aagtcatcgt
     3661 tgcctttttc tttgaataca tagtctacca tattgaaatc tggataagtt aatggatgaa
     3721 cttcgatttc tttgtctcct actttaaatg acaagtcatt caagaagttg tagaaacgat
     3781 gtgcaccttc gattgatgca cccattaatt taccatatcc tgtgacattc aaaggtaaga
     3841 catggtgtgc tgcccaaacg ctggctgctg ttgcccctgc ttttgaacct tccaagatat
     3901 aagccccaag taatgctggg atatctgcgc ctttttcaaa tacatatgtc gcaaagtaag
     3961 agataacgtc tctcatgcgg atatctttga tgacgatacc accggcagaa tatggaacat
     4021 agcccatttt atgtgggtca atcgttacag attctgcttc ttcgattgct ttatacgcac
     4081 tgtgtacttc ttctaagata taatctttgt tttctgtaaa gacattgtat ttgtaatgaa
     4141 catcttttaa atcttcaaat ggaatgaagt tgttgtcttc gtctaagaaa atcgcacggc
     4201 cgtatccgcc ataagcagca tctacgtgta agtagaagta aataccgtct ttttctaata
     4261 cgcgacgcaa tgcaacgatt ttatcgatac cgtcgattgc tccttcttct gttgatccta
     4321 caacaccgac tacacctagg attggtgttt tttcagctgc tagaccacga acgatttttt
     4381 caagttcgtt gatgtccatg cgatagttgt ggtcaactgg cactggaatc acttggtcta
     4441 aaccgatacc gatgatgtcg gcagctttta gccatgaata gtgtttagtt tgtggaacca
     4501 accattttcc taatttttct aaatgttttc cgctacgtgc tgagtgagct ttgatttcgt
     4561 cgattttttc tgggacgctg tctaacagat tcatgatttc ttctgttgac aagttcatta
     4621 attcccaatc gctttttcca gcaactagtt caggtgtcac ttctttcatt gcaagcggca
     4681 atgatttgat gttgcgggca taccaaagtc cttctaagtt tgctaaagaa ccatcagcaa
     4741 cgatgtggcc ccagccatct ttatagctca ttaattttgc aaattccatt cctacttctt
     4801 cttccatttg gcttgttgca ggagaagatt cataggcaac gttattacca ttccatagca
     4861 tagcaaaatt gtacgctagt aaagatggca tcaatgtttc agagttcata tgaccccagt
     4921 aacgtccggc attatgccaa ggaacggaat gggtacgcat ccgcgctgaa atttctgata
     4981 gaacatcctt tgttttattc acagtgtgtt caaaactagc actgcttttt tcttctggcg
     5041 taataatagg catgtcttga ggcatgtagt tttgacgcca gcctaaatgt tcatccacca
     5101 attcattcaa caaagcttta tagatttggc cgttctcagc tttgtcccca atgaataggg
     5161 catttaggtt tagatctttc gacaatgatt cactcatagt gaaaccctcc taaaaaaata
     5221 tttaaatttt gtcgagccct acattacacc tgttcttcta ttctgtaaac taagttttgg
     5281 cctgattttt aatcatattt taatatgtat caattatccg aattgttcac acttttgaaa
     5341 taattaaaaa aaacgaggga atttacaaat gtaaactccc tcgcaaagat tgtattaacg
     5401 acaaccagcg ttctatttta ggatgggttt caaaattgga atgttgtaat agtatttccc
     5461 tttttcaagg taaaaactta ctaaattcta atcaattact ttggccaaaa agtaattttt
     5521 cttacctttt cgaataacca caaacttacc atcgaattca tcggatggat ttatgacaaa
     5581 atttaaatcg gtaacgcggt caccgttaat acttatagct ccgtttgaaa catcttcacg
     5641 tgcttgacgt ttagatggtt caatttttgt ggaaacaagc agttccacga tattttcagg
     5701 tgtgcttgaa atttcaacgt tcggcatttt accgaatcct tgcgcgattt cttccgcatt
     5761 tagttcttta atatttccag agaacaatgc ttgcgttatt ttttgcgctt ctttcaagtc
     5821 ttcttcacta tgcacaaatc ttgtaacttc ttctgccaaa cggcgttgtg cttcacgttt
     5881 ctcaggttct gtctctactt ttttcgctaa gtcttcgatt tcttcttgag acaagaaagt
     5941 aaagaatttc aagtatttca ctacatcgcg atcatcttga ttcaaccaga attggtagaa
     6001 ctcaaatggt gaagttttct tcggatcaag ccaaacggca ccgcctgcag tttttccgaa
     6061 tttcgttccg tcagccttca gcatcaatgg gatcgttaaa ccgaacgctt ctgcttctgg
     6121 tccttctaat tttcggatta ggtctaatcc agctgtgata tttccccatt ggtctgctcc
     6181 accgatttgc aattgcacat cgtatgtttt atgcagatgc aaaaagtcga tagattgcag
     6241 gatttggtaa gtaaactctg taaatgagat tcctacttcc aaacggctgg caacgatatc
     6301 ttttgctaac atcgtgttga tgttgaagtt tttaccgtag tctctcaaaa attcaagtaa
     6361 agagattttt gacaaccagt cgtagttgtt cacaaatgtg atatttgcat ctttaccaaa
     6421 caattttctc atttgattgg aaagtgcgtc cacgttatgt tgcacttgtt ccatcgtttg
     6481 caacacacgt tctgttttgc ggccgcttgg gtcgccgatc gttcctgttc cgccgccaat
     6541 cagaatatat ggatgatggc ctgccaattg gaatcttttc atcatcataa atggaatcaa
     6601 atgtccgata tgcatcgaat caccagttgg atcaacgccg caataaagtg aaatgctctt
     6661 ttcttcaacg agctcgcgaa gtctttcttc gtttgtttgc tgattaatgg cgtcgcgcca
     6721 agttaattca tcaataatat tcattttact gacctccgtg tagttttttt atgaaagcag
     6781 gaacctgcta gcatgtgtaa ttaattattt cttcaggata ggataggaaa taaaaaaagt
     6841 cccttggcat ataagccaag ggacgaaatc tcgcggtacc acccaatttg tatgtctaga
     6901 tatccaacat acctctcgaa catttaacgc tgtcgcgtgg caatcactgc caaagactcc
     6961 tgaaatgtaa ttcgctttaa agcatgtact ggtttgcagc gaccaccagc tttctgaaac
     7021 attggcttta acaactactt cgtttcgatc ttcgtcttgt tattattaat atgtgtctat
     7081 catagagcgc aactttttct ttgtcaacag aaaaaggata ttttttttaa attcctcatt
     7141 ttttcttttt tgaatccgct ctaattccga acattttatt ttcaattatt gttaaaagaa
     7201 ataacttttt ctgtattttt tctgcaattt atcgagaagt cactttactt tgcctcttat
     7261 tctttcttcg ttacaataag gaatgggaca agtaaaaaaa tgaaaaagca agggacgagg
     7321 tatatggaaa aaaagcaaaa aattatttta ggagccactt ctgctttaac tatcgttatt
     7381 ttaattctaa gtttgttttt tgtcatccga tcatctacaa aaaaagaacc aatcattcct
     7441 gaaaaaaaaa taacagaaaa agcaaaagaa ccgacagatg ataagcaaca actagttact
     7501 caagcaaaaa atctggctca aagctacgat tatgaccaag cgattgcgtt attggaaaaa
     7561 gatagtaaga acggatctac tgaaacacaa cagcttcttc aaacactaaa aaaggagaaa
     7621 gaacaactga tcgactggtc tgatcctact cagatctctc atgtcttttt ccacagcttg
     7681 atcgtggacc cgcaaaaagc cttccactct caacaggcac aaggatacaa agattatatg
     7741 gtgactgttg aagaattcaa ccgttcgatc gaacaacttt atcaaaatga ttttgtttta
     7801 gttaatttga atgatttgat ccaaaaagac gaacaaggaa atctttcctt taacgggctt
     7861 gcgctacctg aaggaaaaaa accattggtt ctttcacaag atgatgtcag ctattatgaa
     7921 tatatggata attccggttt tcctagccga ttggtcgtat caaataacaa tgaaatcaaa
     7981 aatgtctata aagagaaaga taaagaagaa atcggtgact ttgacatggt tcctctgatc
     8041 gatgcctttg tcaagaaaca tcctgatttt tcttatcgag gcgcaaaagg taccttggcc
     8101 ctgacaggtt acaacggagt tttaggttat cgaacatcaa aatctcaata tggagacaat
     8161 gaaaaaacaa aaaaagagat taaagaagct aaaaaagtgg cggaacagtt gaagaaagac
     8221 ggctggagct ttgcttccca tacatggggt cacttgaata tgactcaagc ttcactagaa
     8281 gatgtaaaaa aagacaatga attgtggcag caagaagttg cacctatcct aggaaaaaca
     8341 aatattctga tttatccatt tggtgcagac atcagcgact ggcagcctta ttcagcagaa
     8401 aatcaaaaat ttgcttactt gaagagtcaa ggtttcgata ttttctgtaa tgtcgatgct
     8461 tctacaccag cttggggaca attaggtacg gatttctatc gaaatgctcg gatcaatatc
     8521 gatggcattc gttttcaatc tgatttagac ggaaaaaatc ctattcttga tccattcatc
     8581 aatgtccatg atgtctatga caaagaagca cgcgaatctg cttgatttta cgtcatttat
     8641 gaaccaaaaa agccgaactg acattttatc gtcagtttcg gcttttttat gctttatttc
     8701 ttcttcggtg cgattccctt gatcaggact tctgtccatt ctgctgtata tggaagcagt
     8761 tttgcgtata tctccggctt agaaattcct tctaccattg cttgaaaata gaggatcaag
     8821 aactcatccg aatatttcaa atccactttt ccttctttcc gccctcgatg gaaaagatca
     8881 agcatgatac tgaaactttc ttgcgtatac gcatccatcg cgtcagaaaa ttcttttttt
     8941 tctttttttg tatagtatgt catcaaatcg atataaaatt gtttgttcac tcgattgaga
     9001 taacggattt tgttttcact catggcaatc agtgtttctt caaatggaag attttccgca
     9061 atcaacgctt tcgcttcttg ccctaaatca ttcataaatg caaaaaatac ttcataaatc
     9121 agcccttcct tgctgccaaa atatttaaaa atcgtcgctt tacttacctg agcgttctca
     9181 gctaccttat ccattgtcac tccagcggtt cctatgtctg tgttcatcaa gtcaaaagct
     9241 gcttgtaaaa tctctttctt tttctcttgt gctctctttt caaaaccgtt catttgtaga
     9301 gcctcccgta attttaaact tttataatat atatagttca aatttattct gaggtttatt
     9361 ataactatct attgagaaag tatcaagcta gcaattcctt gtttatgaat tcattgacag
     9421 aaatttataa aaagaatatt atgtactaaa ataataaaaa aagttttata aaaaggtata
     9481 aaaccttttt attaaactat ttaatcttgt ttagtatata tttttagaaa aaggagtggg
     9541 gtttttatgg cagaaatcgt caaagtcaca catttgaaaa agagctttgg tcgtttcgaa
     9601 gctttaaaag atgtctcttt caccgtacat tcaggtgaag tcgttggatt tatcggacca
     9661 aatggttctg gaaaatccac gacgattcgg acacttttag ggattataaa aaaagacggc
     9721 ggaacggcaa cgatttttgg gcaagatgta tgggaagata gtttatctat ccatgaacgt
     9781 ctttcttatg ttccaggcga tatctctttg tggggaaatt tgactggagg agcgatcatc
     9841 gatctcttca tgaagcttca tggaaaaggg agtgctcaaa aaaaagatga actgattcaa
     9901 cgtttctctc ttgatcctaa gaaaaaagca aaaaactatt caaaaggaaa tcgccaaaaa
     9961 gtgggcttga ttgctgcttt ggctgttgat tcggatctct atttatttga tgagcctact
    10021 tctggtttag atcctttgat ggaaacgatt ttccaagaag aagttgaaaa attgaaagaa
    10081 cagggcaaat ccatcctact ttcttctcat attctcagtg aagtcgagcg actagcggat
    10141 aaagtcgtga tcatacgcga aggaacactc gatgaagaag gaacactcga tgaattgcgt
    10201 catttgactc gttcgaccat cactatagaa acagaagcag agaatagtca cttagcacaa
    10261 ttgaacggtg tccatgactt tgtgcaaaaa ggtcacctcg ctcaatttgc tgctgataac
    10321 gaagctttcg atgtgatttt agctgaagcg tccaaattag gtgtgaaaaa atttgaatct
    10381 attccaccga ctcttgagga tttattcatg agacattatg aaggatgagg aggaagcgaa
    10441 atgactgata aattttcacg ctggtccacc cttttttctc aaaaccttag ccgtgactgg
    10501 aaaaagatcc tgatatggct agtcggtgtc actgcttttt ctggtggttt tgtccctgcg
    10561 tttgaagaat tgacttccgg acaaggatta gctggattat acgaaacctt gcaaaacccg
    10621 gcaatgattg caatggtcgg accaactcca gcttcgaatg ctgctgagta tactttagga
    10681 gcgatgtatg caaatgaaat gttgcttttt tgcagtttgt ttgctgctgt gatctctgtt
    10741 ttacacgtgg tgagccaaac gagaaaaaaa gaagaacttg gtctgattga acttattcgt
    10801 tcctttcgag tcggacgtca agcaaactct ttagctgtca tcgctgaaag tattctgatc
    10861 aatttgcttt tagcaatatc gatcagctgt acgatgattt ttttccaagc ggattcgatc
    10921 actgcagaag gtgcttggct ttttgggcta tctgtaggta gtatggggat tttggggagt
    10981 gccgttgcac tgtttgctgc acaactgatg ccaagttcag ccagttcgaa tggtctgtct
    11041 ttagcgtttt taggtggact ttaccttcta cgagcaataa cagatgtagc tgattcctcc
    11101 ctttcgatgt ggaatccttt aggctggtct tatttgacct atccatttac aactaacaac
    11161 tggattcctt tgctttattt gtttttgttc agtctaatct tgataatcag ttcatttgtt
    11221 ttagaggaac atcgagattt gaacaccggc tatatccccg aacgtgaagg aaaaccgtat
    11281 gctaaacgtt ctcttttatc cttgcgtggt ttattattct atctaaataa agggatgaca
    11341 atcagttggc ttatcacttt tggtttgatg ggggcggcat acggctctat ctacgggaat
    11401 atgcaacagt ttttagaaag taacgactta atgaaacaga tgtttatcca atctggcgtc
    11461 tcaattgaaa aaaactttac aagcacgatc atggtcgtct tgatttgtct agctgctatt
    11521 ttaccaattg ctagcatcaa tcgtttattt acagaagaaa cccacggacg tatgaatcag
    11581 ctgttcgcaa caaaaatttc taggaaccaa ctttattgga ccaatatgtt actagctatt
    11641 ctatttgcta tattggcaat cctgattagt tctggtgcac tagggctttc tgctttggct
    11701 tcgatgaagg atgcgaaaga catggatcta ttgatgtttc ttaaagccgg gttcaatttc
    11761 ctgcctgccg ttttagtgtt tatcggaatt agtggctttc tattcggatg gcttcctcga
    11821 atcggtaaac ttgcttatat ctacttagct tactcctttg cactcaatta ctttggcgga
    11881 attttggatt taccagaatg gttttccaaa acagctgtcc ctagctgggt tccacggatg
    11941 ccaaacgatt cttttgatct ttccgtgttc cttacgttat catgtatcag ccttgcactg
    12001 ttcgtccttg gttctattgg ttataggcga agagacttac tagaaggata ataaaaaagc
    12061 atgtacaatt tcatgctagc ctccaaatct gggtcaaaaa tccaacattt ggaggtcatt
    12121 tttttattta caagtcttct aactgctctt tatcaatgat ccagacttct cgattctttc
    12181 tttttacata accattctcc tctaataatt taaattttct agataaagtt tcgggggtcg
    12241 taccgataaa tgcagcaagt tctttcattt tcattggcag gaaaaagtga tccttccctg
    12301 tcgctttagc taagtcaagc agatagacag cgactcgttc ttctactttt tccatcatca
    12361 aaaaagttgt ctgctgctgt acttttgtta atttctcaac gttgattgct aaaagtttca
    12421 gactaagggc gggataatgc agtaagagtt ccgagaaatc tttttgtctc aatatgcaaa
    12481 tttccgtttt ttctagtgct tcgccaaata aagactcatt tggaacgcct agcaactggc
    12541 tctctccctc atatccgccc ggctctatta ctcgtaaaag ctgttccttt ccgctagcag
    12601 ataattgata cactttcata ctgccggagg ccacgatcac taattgaggt tctccattcg
    12661 gcgcaagaat ctgttctcct ttttccacat agtgatgttt gacaagtgcg tgtacttttt
    12721 cttgatcttg ctgtactaag tgattgaaca aaggaacaaa tgaaacacat aaatgatcat
    12781 gactcataat ctctcctcct tctaaacagc tatttctaat aaagaaacat aacttccctt
    12841 attccaactg ataattttct tttatcatag cataaacgtc aagcgaacca gataccagca
    12901 aatagagtgg atttcttttt gtcccttgct ttattttcct tcttttgaga ccttaagtaa
    12961 ttgattccta tcaatttttt aaatatttac tttttgaata atcaaaaaca aaccttcact
    13021 cgcaagccga gagtaaggtt tgtttttcgc attgcttatt tcgtgttgtc tattgaaatt
    13081 tttccgcacc tagcttttga tatagtggtg ctagttcttg taaggtttca gcggttattt
    13141 gctcaacgtg ttcgatttct tgcggataga agaatagttt acccatttga aaggttcggt
    13201 ctctcatcgc ttttttcaac tcttcttgtt tctttgtatc ggacaaatag tcagacaaag
    13261 ctaagtggtc gtcaagttca tgttctggtt gcggccatat gtaataatcc ttgatatcga
    13321 ctgttttgct ccactctttc agtatcgcaa gcaactggtt atacgctgca atgctaccat
    13381 atcgttcttc gcttttataa tgttggtaca tcaaatagat ttggtattgt ttttgattca
    13441 acaaaactgc tagacaggca ttctcgtctt gacgagattc gcttcggtac gcgctccaaa
    13501 aatgactgcg taaattccag ccattcgtcc agctttcgat tttaggtttg ctcatgccgt
    13561 acatatcagg caaaagtgca gctgtctgga gctgaatctc tttccatttt tgccagtgtt
    13621 ctttatattc tttcttgatt tgttcaatct tatctgttgg atggctctct ttgagctgtt
    13681 tgaatgcgta gattggtcga tcaaacaact gataatctga ttttccaagt tccattgttt
    13741 cgcctctttc cgttatcttt tgtattctac ctgaattttg cgatatggac aaatagaggt
    13801 tgtgaaaaag ctgttctgca tcaagtcata agaacagcca aacaaaaaac tgcgatgagc
    13861 atcaccgcag ttttattgaa cttaaggttg attgatcaca tctaaaagcg ttgaaaataa
    13921 aatggcacta gaagcagctc ctgggtcgat atgtccgatt gcacgctctc ctagatagga
    13981 agctcgtcct tttttagcta ccaagacttt ggtgtgttct tttgcctggt caatgatttc
    14041 ttttgttaaa ttccctgcct tcaatgcctc tgtgactggc agccaaacat ctaacatcgt
    14101 tttatcgtca gcttctgcct ttcctcggct ttgaataccc gctgttccct gctcgatcac
    14161 ttcgcctaat ttttcttgag aatcaataga atcgacctct tttgtcgctt tcatcatgtt
    14221 catgaatgca gatccgtata acggaccaga agccccaccg actttcgata tcatcgccat
    14281 agagaatact ttgaatgtat cgctgattgt ttcaggatga tcggcttgaa aagcttcttc
    14341 ataagctgaa acccctcttc ccatgttatt tccatggtct ccatcgccaa tcggggtatc
    14401 tagatcactg agatattgtt tgttctgatt gattgcctct gcaaattgcg tgagccattc
    14461 ttggatttct tgaacggtta gtttcatatt atcacccttt tatcttgaga ttttttacca
    14521 tgaaatcgtc tgtacattgc tttctaaaga atctttccat tgtgtctccg tcaagtcgat
    14581 cagtgttaga gaaagacctt gcatatctag tgaggtcata aaattaccga cttttcggaa
    14641 aactacattg acgcctcttt cttccaacag agctaaaaca tcattcatga acacgaactg
    14701 ttccatcaaa ggcgtcccgc ccattccgtt gactagtaca cctgcttctt ttggtaaatt
    14761 cgagtaatca tctaaaatct tagtaacaag ttcctttgcc agtactttag aaggctgcat
    14821 cttttctcga cgatagcctg gctcaccgtg gataccaaca ccatactcga tctcatcttc
    14881 tcctagttcg aatcctggtt tccctacttc tggtacagtt gctgctcgaa gcgctagacc
    14941 gatcgtctta gttgcctgta cgaccttttc tcccagttct ttcaactcag ctagggaagc
    15001 accgtttctt gctgcatcgc ccacgatttt gtgtacaagg acagtacctg caactccgcg
    15061 ttttccagca gtatacgtac tattttcaac ggcgatatca tcatctacga tcacactttc
    15121 tacttcaata tcgtccatag cagctaattc ctttgccata tcgaagttca atgcatcccc
    15181 tgtataattc ttaacgatca gcaaaactcc ttttccacta tcggcttcct taatagccgt
    15241 ctgaatctga tctggagttg gagaagtaaa cacatcacca agcaccgctg cacttaacat
    15301 cccttcccct acaaacccgg catgggatgg ttcatgtccg cttcctccac cagaaaccaa
    15361 accaacttgt tccgctttct gattcttggc tacaacacgg gaagattcta cacgatgaac
    15421 aagttctgga tagcttttta ctaatccttc cagcatttct tcgacaatat tgcttggatc
    15481 attcatgatt tttttcaatt tattcccatc ctttctttcc ggcattttgt gcttctgtta
    15541 agatttgctg agtatcatct gttataccgg cagtgatggc tgctgcaaaa gctccttcca
    15601 ctaaaggcgc atccaccaga tgataacgtt gctgctgctc ttcttccaac atgtcttttg
    15661 ccagttccga gttcaatacc gcgctaccga tatccgcaaa aacaaaataa ctagctgcat
    15721 cgcttgattc attgactgct tctacgattt tcatcgggtc tgatccgatc tcgccattta
    15781 ttccgccgag agaaaaaatt tgcacctctt cggatttttg catctgctca atcatttctt
    15841 taataccatc tgttactttt tgactatgag aaataagcaa aatacttttt ttcacaagaa
    15901 tcttcctttc ttttacatag ttgctaacga ttggtttcgc tttcattaat gaacaattca
    15961 agtatataat aaat
//
LOCUS       DACGLE010000063        15962 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0534
            SAMEA3304449-rid9560453.denovo.067, whole genome shotgun sequence.
ACCESSION   DACGLE010000063 DACGLE010000000
VERSION     DACGLE010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304449
            Sequence Read Archive: ERR985064
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15962)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15962)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702225.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702225.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 20:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,045
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 27 of 67
            Pseudo Genes (incomplete)         :: 25 of 67
            Pseudo Genes (internal stop)      :: 19 of 67
            Pseudo Genes (multiple problems)  :: 4 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15962
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304449-rid9560453.denovo.067"
                     /strain="VREN0534"
                     /db_xref="taxon:1352"
     gene            complement(<1..450)
                     /locus_tag="IXT20_002089"
     CDS             complement(<1..450)
                     /locus_tag="IXT20_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAQ4860525.1"
                     /translation="MKYKEMFVPSLIQLAVEVKNKEEAFLIAAQQLEKMGYVNENYLN
                     GLIAREREFPTGLITKYLNIALPHSECEYVEKPFVFVMQLKNPIYFYQMGDNQEMTVE
                     NLFFLGIKEPKEQVGLLQAFMNLFMDETFVENYKKQETPQNIYELITN"
     gene            674..1465
                     /locus_tag="IXT20_002090"
     CDS             674..1465
                     /locus_tag="IXT20_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAQ4860526.1"
                     /translation="MLKKERQEQIITLLEKDFYLTNSEIANKLGVSEMTIRRDVTELA
                     KKNKVKKLYGGVERKEPYNRELSTQEKIDTNVAEKKYIGQGMNSMIPDNSTIYVGAGT
                     TILYALPFIQKKNLFVITNSLIAFNYLKNHTSYRILLTGGEFSPITEEFIGEVAERSF
                     ESLNVDISFAATNGIFDNNVTTAQFIEGNIQNAALNAAKIKCIVADYSKLNKSDVYTF
                     RKLTDFDYLITDNHLSEQDFNHYSKYLTILKEPKNDTDNHDEPIH"
     gene            1431..2384
                     /locus_tag="IXT20_002091"
     CDS             1431..2384
                     /locus_tag="IXT20_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase family hexose kinase"
                     /protein_id="HAQ4860527.1"
                     /translation="MILTITMNPSIDSIYFTKHFELGTMTRFDSPVRYVGGKGINCGR
                     ASALLGSSVILTGFLGGYFGSMIQDMLKNENLFDLHFLPIAEESRGAVTIMHDDETQT
                     ELVEAGPEIADTTIKKLMNDIKLISKNNYLSVITINGSVNNPDPFLYAKLLQYIKEQI
                     DPSTYILMDISGQHLREVLASKYKPMFIKPNIHEFSDIIHQKITQKSDIKHHLKTNPL
                     FKDIDVIMISCGSYGAVVKVKEEFWNVEIPKIPIINTTGSGDATVGGFAHSLEQNYPL
                     KECVRYAMACGMSNAQQSEVGKVIPADVKNLAKSIKITEMK"
     gene            complement(2782..3756)
                     /locus_tag="IXT20_002092"
     CDS             complement(2782..3756)
                     /locus_tag="IXT20_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAQ4860528.1"
                     /translation="MNKELNITQEWDKVFPKSNQVNHRKVSFPNRFGITLVADLYEPK
                     EVSEKKLPAIAVSGPFGAVKEQSSGLYAQTMAERGFLTIAFDPSFTGESGGVPRYVAS
                     PDINTEDFQAAVDFLSVQKNVNPERIGIIGICGWGGMALNAAAIDTRVKATVVSTMYD
                     MTRVTANGYFDVMDENARYEMREELNAQRIIDFKQGDYARAGGVVDPLPEDAPQFVKD
                     YHAYYKTDRGYHVRSLNSNDGWNRTSGLSLMNAKLLHYIPEIRHAVLVIHGEKAHSRY
                     FGEDAFKELKGDNKELMIIPGASHTDLYDQTDIIPFEKMATFFEENLV"
     gene            3871..4305
                     /locus_tag="IXT20_002093"
     CDS             3871..4305
                     /locus_tag="IXT20_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAQ4860529.1"
                     /translation="MYSIGQVSEMFGLPVSTLRYYDKEGLFPDIQRVSGIRKFSENEL
                     EALRVIDCLKKSGLEIKDIKQFMEWSKEGSKTFEVRKELFEKQKEVVEKEIAKLERVL
                     AMLNYKSWYYEEAIKAGNEEAVLTMIPDDLPQHVKEAYVHSH"
     gene            complement(4430..5416)
                     /locus_tag="IXT20_002094"
     CDS             complement(4430..5416)
                     /locus_tag="IXT20_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HAQ4860530.1"
                     /translation="MEQGSLVKSAQDAYLQSIDLAVEAEKIGVDGAFFRVHHFAPQIG
                     SPFPLLAAIGAKTSKIEIGTGLIDMRYENPYYMAENAGLTDIITQGRVQLGVGRGSPE
                     QVLDGWKYFGYDYSEEEIKEVTRERTLEFLELLEGKPFEEPNPQPMFPQGSGKLRIEP
                     YSEGLRQRIWWGAGSQQTAIWAAQHGMNLQSSTLVNYESHEPFHIQQANQLRAYKEAW
                     KQAGHDFEPRTLVTRSIQPITTDLDRRLFGQDGSPQQDAVGYLAYHRNPTIFGKTFAG
                     EPDQLIKELAEDEAIKEADTVLVTIPNTLGVEYNMHLLETIVKDIAPELGWR"
     gene            5844..6266
                     /gene="arsC"
                     /locus_tag="IXT20_002095"
     CDS             5844..6266
                     /gene="arsC"
                     /locus_tag="IXT20_002095"
                     /EC_number="1.20.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (thioredoxin)"
                     /protein_id="HAQ4860531.1"
                     /translation="MKKIYFLCTGNSCRSQIAEGYARKYLSPSMFEIRSAGIETHGLN
                     PRAIKIMAEDGIDISKQTSDLIDRDYFTQADLIITLCGDAKDKCPAVPNNHEHLHWDL
                     KDPAQAVGSEEEILTEFRKVREGIKRKVLDLNQRTSIV"
     gene            complement(6546..8468)
                     /locus_tag="IXT20_002096"
     CDS             complement(6546..8468)
                     /locus_tag="IXT20_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HAQ4860532.1"
                     /translation="MNQRQQSLIYQLSENNNYITTKKLAEHFHVSERTIHSDLNTIET
                     WLRDKHLPLMFERKKGTGILLNGLPHEREQLKRALNEETQTEMDKKQLRQLLIFHLLV
                     AKDGFSLEELSEKLYVGKRTIRKELTELQSFFEYHHLQLVSKTKLGTFLKGDEQEKRQ
                     LLVKTLRNMQKEDPQSPSLKEFFAKDTLKVIQHTLQDVFYENHLEQPNGLASVEIHIY
                     FMLERMKQYQKVKLSKDENEVVEHTQAQKLSSQILAKLATIYPIEFSPDEINYLALRI
                     ANLFTNSQAATRFQKESSALADHLIEQVEQFLGYSLKEDQLLKQNLRSHLSSTYFRLH
                     YGLQISNPLTKNVFSTYTQLFLVLQLILEDYFEKENFYVPQEETAYLTIHFQAAIERH
                     KHQKSRRYQTVLVSEYSKAMATFLEARLNRELPELTIMDLVEYKPELKRQDFPSVDFI
                     LATLPFKHISIPVIEISPMITETDLAYLTKYMLEHVPIKKKKTFDLASFTHPFLIFPQ
                     LERTDPADILNFMGNVLVEHHYVEPEFIDSVLERDSHSSTRVAPFVTIPHGNPLYVKH
                     SMISIATMKEPVLWHGEQIRIVLLLAIKKNDLKHAEFKKIFSVIHYLEKQPEQMAQLM
                     QSNHPLEILTLLSSYE"
     gene            complement(8555..10495)
                     /locus_tag="IXT20_002097"
     CDS             complement(8555..10495)
                     /locus_tag="IXT20_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIA"
                     /protein_id="HAQ4860533.1"
                     /translation="MKKIIAITSCPVGIAHTYMAAENLEKAGKAVGAEVKVETHGSIG
                     IENELTARDIEEAAGVIIAADTKIDKSRFGGKPLITVGVQEGIHHADELVRDILAGKA
                     PVYKSTEAIISSENKSESENLGKKIYKSLMNGVSYMVPFVVTGGLLIAISLTLGGTAT
                     PEGIKIPEGTIWATMNSIGSIAMGLMVPILSAFIAQSIADRPGLVPGFVGGMLAANGA
                     LYGSDANAGFLGGIITGFLAGFIALGIKKVRVPKALQSIMPIIVIPILGSLAVGFVFI
                     YVIGEPVALLFSSLTHFLAGMQGGSEIVLAMILGAMIAFDMGGPFNKVAFLFGSGLIA
                     EGNYSIMGPIAVAICIPPLALGLASLILKTKFTKTEREAGKASLAMGLFGITEGAIPF
                     ASTDPLRVIPSIVIGAMTGSVIAMLSGVTDHVAHGGPIVAVLGAVDHVAMFFVAVIAG
                     IAVTVLMLRILKPTLQQTAVAAANGVTVADLSETETSDVYTKTETFDSITDIMSDEAI
                     VLGETATTKDGVIEDLVQRLEKTAAITDKTRFKEVIYDREHESTTGIGMGIAIPHGKS
                     AYVLKPTVAFARSQSGVDWHSLDGKPAHMIFMIAVPENSQGDMHLKILQRLSRKLMDD
                     DFRQALMEAPDKTTVHQLLSEM"
     gene            complement(10783..11523)
                     /locus_tag="IXT20_002098"
     CDS             complement(10783..11523)
                     /locus_tag="IXT20_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KDGP aldolase family protein"
                     /protein_id="HAQ4860534.1"
                     /translation="MSVIPNYYKNRVCLNVLAHSVENAKACYEAAEGHVVLGVLSKNY
                     ASDEAAIEDMKRYQDATNNALSVGLGAGDPNQSQMVSRLSGVLQPQHVNQVFTGVGTS
                     RALLGQNDTVINGLVSPTGKIGYVNIATGPLSSQAPAAEVPIETAIQMLQDMGGTSIK
                     YFPMKGLAHREEFKAVAEACAKYEFYLEPTGGIDLSNFEEILKIAIDAGVKKVIPHVY
                     SSIIDKETGDTRIGDVKILLSMVKKIIH"
     gene            complement(11536..12627)
                     /locus_tag="IXT20_002099"
     CDS             complement(11536..12627)
                     /locus_tag="IXT20_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010771059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DgaE family pyridoxal phosphate-dependent
                     ammonia lyase"
                     /protein_id="HAQ4860535.1"
                     /translation="MTISYGKFDLKEVINASGKMTILGVSKVSESVLAAQRFGGEHFF
                     EMSDLAIKTGAYLSQLLKVEDAQIVSSASAGIAQSVAALIGQGDMYHVYHPYTEKIAR
                     REIIIPKGHNVDYGTPVEVMVAQGGGKVIEAGYANMCTPEHVSMMITEKTAALLYIKS
                     HHAVQKSMLTVAEMVEVAKAHRLPLIVDAAAEEDLFKYSEMGVDLVIYSGAKAIEGPS
                     AGLVIGKKKYIQWIRLQSKGIGRAMKIGKDNILGFTQALEDYLKNGSESGDSMKQRLA
                     PFVNAINQLPDLSAKIVQDGAGRDIYRASVTINGDKSAKEVIQELKARNPAVYTREYQ
                     ANNGIIEFDIRSVSQNEMEKIIKRLREIM"
     gene            complement(12617..13723)
                     /locus_tag="IXT20_002100"
     CDS             complement(12617..13723)
                     /locus_tag="IXT20_002100"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase/deacetylase family
                     metallohydrolase"
                     /protein_id="HAQ4860536.1"
                     /translation="MFDTLIKNGRLVDGSKIEVAIEKGMIKAVAAEINQPAQEVVDLE
                     GKYYLSAGWIDDHVHCYEKMNLYYDYPDQIGVEKGVTTVIDAGTTGAENIREFYQLTK
                     SVKTNVYALMNISKWGIVKQDELADLTKIQEDLVSTALAELPEFIVGIKARMSKTVVG
                     ENGIKPLELAKKIQTENNDLPLMVHIGSAPPELEEVLSRMERGDVLTHCFNGKPNGIL
                     DPKTDQIKNFVWDAYNNGVVFDIGHGTDSFNFHVAETALKAGMKATSISTDIYIRNRT
                     NGPVFDLATTLEKLRVVGYSWEEIIEKVTAVPARNFHLKNKGQLAVNYDADLTIFEIV
                     ENEKELKDSNGFTRIAKEQILPIKTIIGGVIYDN"
     gene            complement(13742..14419)
                     /locus_tag="IXT20_002101"
     CDS             complement(13742..14419)
                     /locus_tag="IXT20_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4310 family protein"
                     /protein_id="HAQ4860537.1"
                     /translation="METTEEQAKVLEAKRQKSFWFADWSFPLIVGIMSAAVFAGTHMY
                     VMYGVGAFNEVSIVAMLKAGLDGGSYGAAAAFGASFLFARILEGSLVGILDLGGSILT
                     GIGIGVPAILLSMKIAAPIDNFALSLLTGLVLGLIVGGIIVLIRKFTINQSNSTFGAD
                     VMMGAGNSSGRFLGPLIILSACAASIPIGIGATLGALGFYAWKKPIAGGAILGAMILG
                     AIFPAVN"
     gene            complement(14430..15209)
                     /locus_tag="IXT20_002102"
     CDS             complement(14430..15209)
                     /locus_tag="IXT20_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4311 domain-containing protein"
                     /protein_id="HAQ4860538.1"
                     /translation="MDFVIILVKSVIIGGLLGFAASVGAARMFHAPNTQGLGAFRTLG
                     EMNACMGDPASHFSFGLGFFFNAWASSVGAGAFTQDVTHRIIPNWAAAALLAKNKDVT
                     QTMHDPKKMGIIGAVIGAVVVAFLNTTAAAIPEALQVTAVSVLVPAATILINTVMPVI
                     FWLAALDAGKRTGFWGTLFGGIAQLIMGNAVPGVVLGILIGKGVDESGWNRVTKSMMI
                     AIVLLFIFSGFFRGFDMEMIKSFQLQVPNWLDNLHNIFSVK"
     gene            complement(15238..15588)
                     /locus_tag="IXT20_002103"
     CDS             complement(15238..15588)
                     /locus_tag="IXT20_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4312 family protein"
                     /protein_id="HAQ4860539.1"
                     /translation="MSVQKTIQQKVIVSGKGETKQHAFASALAEIQNKIMEQSNVLLR
                     IEPVNVDILKAVENKYTERFLFFFFPRQRISYEVELSVTVAVTEIKLADISFSQQMTE
                     DPNGIAIPFVTKKI"
     gene            complement(15604..15960)
                     /locus_tag="IXT20_002104"
     CDS             complement(15604..15960)
                     /locus_tag="IXT20_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4860540.1"
                     /translation="MIKVVIADRMGKGQNVAKGVEAAGGKAVVVPGMGADMRLGDVMQ
                     QEHADMGISFCGSGGAGALTAATKYGYPERHGMRSIEEGITAIKDGKTVLGFGFMDQE
                     ELGKRLTEEFIKKHGK"
BASE COUNT         4737 a         3344 c         2691 g         5190 t
ORIGIN      
        1 atttgtaata agttcgtata tgttttgcgg tgtttcttgt ttcttgtaat tttccacgaa
       61 tgtttcatcc atgaataaat tcataaacgc ttgaagtaat cctacttgtt cttttggttc
      121 tttgattcct aaaaaaaata gattttctac agtcatttcc tgattatccc ccatttgata
      181 aaaataaatt gggttcttca gctgcataac aaaaacaaat ggcttttcga catattcaca
      241 ttccgaatgt ggcagcgcga tatttaaata cttagtaatc aatccagtag gaaattctct
      301 ttctcttgca atcagtccat tcaaataatt ctcattgacg taccccattt tttctaattg
      361 ttgtgcagcg attaaaaaag cttcttcttt attttttact tctaccgcta attgaatcaa
      421 actaggaacg aacatctctt tatatttcat tccaacctcc tcctttttga ttaagtttgt
      481 taatttattg ttctactaag ttcatctttg ttttacaaga acattatatt tctgttagtt
      541 tgttttgtca acgctttcaa aagaaattta ttttgttaaa tttgatctac ttgaaaataa
      601 aatcgtttat ctatataatt aagaagaaca aagtcgaaca aaaattgact tttatgttaa
      661 aggagtaatt catatgctga aaaaagaaag acaagaacaa atcattacat tattagagaa
      721 agatttttat ttaactaatt cagaaatcgc taacaaatta ggcgtatcag aaatgaccat
      781 acgccgagat gtcactgaat tagcaaaaaa aaataaagta aaaaagctat atggtggcgt
      841 tgaacgaaag gaaccttata atcgagaact atcgactcaa gaaaagattg atacgaatgt
      901 agccgaaaaa aaatatattg gtcaaggcat gaacagtatg attcccgata attctactat
      961 ttatgtcggt gctggaacaa cgatcctata tgcacttcct ttcattcaaa agaaaaatct
     1021 atttgtaatt acaaacagtt tgattgcttt caactattta aagaatcata caagttatcg
     1081 gattttactt actggtggcg aattttctcc tattacagaa gagttcattg gggaagttgc
     1141 agaaagatct tttgaaagtt tgaatgtcga tatctctttc gctgcaacaa acggcatatt
     1201 tgataacaat gtgacaaccg ctcaatttat tgagggaaat attcagaacg cagcgctcaa
     1261 tgcagcaaaa atcaaatgca ttgttgcaga ttattcaaaa ttaaataaat cagacgtcta
     1321 tacttttaga aaattaaccg attttgatta tctaataacc gataatcatt tatcagaaca
     1381 ggatttcaat cattattcaa aatacttaac aatattaaag gagcccaaaa atgatactga
     1441 caatcacgat gaacccatcc attgattcca tttattttac aaaacacttt gaattaggta
     1501 cgatgacacg ctttgattct ccggtacgct atgtaggcgg caagggaatc aattgcggac
     1561 gagcttctgc tctactaggt tcatcagtta ttttaacagg gtttttgggc ggttattttg
     1621 gcagtatgat tcaagatatg ctgaaaaatg aaaatttgtt tgatttgcat tttttaccta
     1681 ttgcagaaga atcaagaggt gccgtcacga tcatgcatga tgatgaaacg cagacagagc
     1741 tagtggaagc tggtcctgaa atagcagata ctactatcaa aaaattaatg aatgatatca
     1801 aactaatcag taaaaataat tacctttcag tcattacaat aaatggttct gttaacaatc
     1861 cagatccctt tttgtatgct aaattattac aatatatcaa agaacaaata gatccttcca
     1921 cctatatatt aatggatata tctggacaac atctccgtga agtcttagca agtaagtata
     1981 aaccgatgtt catcaaacct aatatccatg agttttcaga tatcatccat caaaaaatta
     2041 ctcaaaaaag cgacataaaa caccatctaa aaacaaatcc actatttaaa gatatcgatg
     2101 ttatcatgat ttcttgcggc tcgtatggtg cagtagtcaa agtaaaagaa gaattttgga
     2161 atgttgagat ccctaagatt cctatcatta atacaacagg ctctggggat gccactgttg
     2221 gaggttttgc tcattcatta gaacagaatt atcctttaaa agaatgtgta aggtacgcaa
     2281 tggcttgcgg catgtcgaat gcacagcaat cagaagtcgg gaaagttatt ccagctgatg
     2341 taaaaaattt agcaaaaagc attaaaatca cagaaatgaa ataaatagta caaagtaaat
     2401 aattgtttat atgaaatgag acacaaagag actgtgacaa aaaacgtgtg taccgacccc
     2461 caaaagttag attttttggt ctaacttttg ggagtcgata cattgtcaca gtcctttttt
     2521 cgaataaacg gtgttctcct gccaactcct cggtcacaag tcacaatatt caacaaacat
     2581 gagaagctat tttttgaaat tgctccttgt gactcagagt taaacggcag gctcacgacc
     2641 tcttggtata aacggtgttc aaaagcttgc atctacgcta atgagtccaa ccaagtcaaa
     2701 aatgaggagc ttttttggct gtccttatta cttgatgcag ggcagttttt tcataatctc
     2761 ttttagatta aatccttttg gttacacaag gttctcttca aagaatgttg ccattttttc
     2821 aaatggaatg atgtctgtct ggtcgtatag atctgtatgg gatgctcctg gaatgatcat
     2881 gagttccttg ttgtcacctt ttagttcttt gaaagcgtcc tccccaaagt aacgtgaatg
     2941 agctttttcc ccgtgaatga ctaagactgc atgacggatt tctggaatat aatgcaatag
     3001 ttttgcattc ataagtgaca accctgatgt cctattccaa ccatcattgg agtttaaaga
     3061 acggacatga tatccgcggt ctgttttata atacgcatga tagtctttga caaattgagg
     3121 agcatcttca ggcaacggat caaccacacc tccagcacgt gcgtagtccc cttgtttgaa
     3181 atcaatgatg cgttgtgcat ttaattcttc acgcatttca tatcgggcat tttcatccat
     3241 aacatcgaag tagccatttg ctgttactcg agtcatgtca tacattgtgg aaacaactgt
     3301 tgctttgacc cgcgtatcga tagctgctgc attcaatgcc atacctcccc atccacaaat
     3361 accaatgatt ccgattctct cagggtttac atttttttgt acagataaaa agtctactgc
     3421 cgcttggaaa tcttccgtat tgatatcagg cgaagcaaca tatcgaggta ctcctccgct
     3481 ttctcctgta aacgatggat caaacgcaat cgttaaaaag cctctttcag ccatcgtctg
     3541 cgcatataga ccagacgatt gttccttcac tgcaccaaat ggcccgctca cagcgattgc
     3601 cggcaatttc ttttcactta cttcttttgg ttcatataaa tcagcgacta atgtgatacc
     3661 aaaacgattc gggaagctca cctttcgatg attgacttga ttgctttttg gaaatacttt
     3721 gtcccattct tgtgtgatgt ttaattcttt attcatattc tagactctcc tttgatcaaa
     3781 attgacattt gtttttgaat gtattattat gataaaactt aaacttgact ttaggtcaag
     3841 gtaaaaattt tattttagga ggattttttt atgtattcca ttggacaagt gtctgaaatg
     3901 tttggattgc ctgtttctac tttacgttat tacgacaaag agggattatt tcctgatatc
     3961 cagcgtgttt ctggtattag aaaattttca gagaatgagt tagaggcttt aagagtcatt
     4021 gactgtttga aaaaatctgg tttagaaata aaagacatta agcaattcat ggaatggagc
     4081 aaagaaggca gtaagacatt tgaggttcgt aaagaactat ttgaaaaaca aaaagaagta
     4141 gtagaaaaag aaatagcaaa attagaaaga gtactcgcta tgctaaacta caaaagctgg
     4201 tactatgaag aagcaataaa agccggcaat gaagaagccg tattgacaat gattcctgat
     4261 gatttacccc aacatgtaaa agaagcttat gtacattctc attgaacaaa cggataactc
     4321 actctccttc tctatgaact tcatgaaaac atgaataaac acctctaact gattgttaga
     4381 agtgtttatt ttttcactca agtatcctgc acttacaaaa atatcctttt taccgccatc
     4441 ctaattctgg agcgatatct tttacgattg tttcaagcaa gtgcatgtta tattctaccc
     4501 ccaaagtatt aggaatcgtg acaagtactg tgtctgcttc tttgattgct tcatcttcag
     4561 ctagctcctt gattaattga tcaggttcac cagcaaaagt ttttccaaaa atcgttgggt
     4621 ttcggtgata agcaagatag ccgacagcat cttgttgcgg actaccgtct tgtccaaata
     4681 agcgtcgatc caaatcagtg gtgattggct gaatcgagcg agtaactaat gttcttggtt
     4741 caaaatcatg acctgcttgc ttccacgctt ctttatacgc acgtaattgg tttgcttgtt
     4801 ggatatggaa tggctcgtgg gattcgtaat tgactaatgt agatgattgc agattcattc
     4861 catgttgtgc tgcccagatt gccgtttgct gcgaaccagc tccccaccag attctttgtc
     4921 gtaatccttc tgaataaggt tctatccgta attttcctga tccttgtggg aacattggtt
     4981 gaggatttgg ttcttcaaat ggttttcctt caagtaattc caagaattca agggtgcgtt
     5041 cccgtgttac ctctttgatt tcttcttcac tgtaatcata accaaagtat ttccagccat
     5101 ctagaacttg ttcaggtgaa ccacgcccca ctcctagttg cactcttcct tgcgtgatga
     5161 tatctgtcaa tccggcattt tctgccatat aataagggtt ttcatatcgc atatcaataa
     5221 gtcctgtacc gatttcaatc ttacttgtct ttgcgccgat tgccgctagc aaaggaaacg
     5281 gcgacccaat ctgaggggca aaatgatgta cgcggaaaaa cgctccgtcc actccgattt
     5341 tttctgcttc caccgctaaa tcaatcgatt gcaaataagc atcttgagct gatttcacaa
     5401 gcgatccttg ctccatccaa tgtccaaagc ttaaaaagcc gatttttttc aaggtaatga
     5461 cctcctttaa acctacaaaa atggaaggtt ttttattttt aatatgaact gattctacta
     5521 ctttttttct taaaagacga ataattgttc tcgcctcaca atcaagaaat gtatcggctt
     5581 actacacccc tactttcttc atcatctgcc gtgtcttaat ctcttttaac agtctgttac
     5641 atcgttgatt tttgactcaa aatgattgtt tctaacatgt atttatggta aaaaagacaa
     5701 gattagttct ttgtacacat cgatttatac gctgaacaaa aagaaatata taaactaaga
     5761 aacccattgt tttttcgctc catcacatag agtttacttt agataaacag tgacatttac
     5821 taatccataa cggaggaaat caaatgaaaa aaatctattt cttatgtacg ggcaattcat
     5881 gccgcagtca aatagcagaa ggctacgcgc gtaaatactt gtctccttca atgtttgaga
     5941 taagaagtgc ggggatcgag acacatggct tgaatccaag agctataaaa atcatggcag
     6001 aagacggcat cgatatttcc aaacagacgt ctgatttgat tgatagagat tactttactc
     6061 aggcagactt gatcattaca ctttgtggtg atgccaaaga caagtgccct gccgttccaa
     6121 acaatcacga acaccttcac tgggacctaa aagatcctgc acaagctgtc gggagcgaag
     6181 aagaaatatt aacggaattc agaaaagtca gagaaggaat caaaagaaaa gttttagact
     6241 taaatcaacg tacatcgatc gtataaagaa gtattcaaaa tgatttttca agcactaaga
     6301 aacaatgtta aaaaaatagc tttccatatt ttacaaaatt gtgccttatt gccgacaaat
     6361 tgtctcttga acaccgttta ccagtagtca agacatcgta agcaactata aaaaattata
     6421 agaaactcaa aataaaagga tgtgccacaa ctttggccac atcctctttt tttgtattta
     6481 gttttctact tccaaataaa tagatagaat tattataaat ataaaggcag ttttttaatc
     6541 gctacttatt cataactaga taacaacgtt aagatttcta gcggatgatt ggattgcatc
     6601 aactgagcca tttgttctgg ctgcttttct agatagtgaa taacagaaaa tatttttttg
     6661 aactctgcat gtttcaaatc atttttcttt attgctaata gcagtacgat acgtatctgt
     6721 tccccgtgcc ataggactgg ttctttcatt gtagcaatag aaatcatcga atgcttcaca
     6781 tacagcggat taccgtgagg gattgttaca aatggtgcta ctctagtact ggagtgactg
     6841 tctctttcca agacagaatc gatgaattcc ggctccacat aatgatgttc cactaagacg
     6901 tttcccataa agtttaaaat atcggctggg tctgtccgtt caagttgagg aaagatcaga
     6961 aatggatgag taaagcttgc tagatcaaat gtttttttct tcttgatagg cacatgctcc
     7021 agcatatatt ttgtcaaata ggctagatca gtttctgtga tcattggtga gatctcgatt
     7081 actggaatgc ttatatgttt aaatggtaac gtagccaaga taaaatcaac agagggaaaa
     7141 tcttgtctct ttaattctgg cttatattca actagatcca taatcgttaa ttctggaagt
     7201 tctctgttca gccgtgcttc gagaaaagta gccatagctt ttgaatattc actaactaaa
     7261 accgtttgat atcttctact tttctgatgt ttgtgtcgct cgatcgctgc ttggaaatgg
     7321 atcgttaaat aagctgtctc ctcttgtgga acataaaaat tttccttttc aaaataatct
     7381 tctaaaatta actgtaaaac taagaaaagt tgtgtgtacg tactaaacac atttttcgtc
     7441 aatggattag aaatctgcaa accataatgg agtcgaaaat aagttgaaga taaatgagac
     7501 cttaaatttt gttttagtag ctgatcttct tttaatgaat agcctagaaa ctgttccact
     7561 tgttcgatca aatgatcagc aagtgcgcta gattcctttt gaaaacgtgt ggctgcttgt
     7621 gaatttgtaa aaagattggc gatacgcaaa gccagataat tgatttcatc aggagaaaat
     7681 tcaattggat agatggtggc tagttttgct aatatttgac tacttaactt ttgtgcttgg
     7741 gtgtgttcca cgacttcgtt ttcgtcttta cttaatttca ctttctggta ctgtttcatt
     7801 cgctcgagca taaagtagat gtgaatctcc acactagcca gtccattcgg ctgttctaaa
     7861 tggttttcat aaaaaacatc ttgtaatgta tgttggataa cttttaatgt atctttagcg
     7921 aagaattctt tcaacgacgg actttgtgga tcttctttct gcatatttcg taatgtctta
     7981 acaagcaact gtcttttttc ttgttcatcg cctttaagaa aagttcctag ttttgttttg
     8041 gatactagct gtaaatggtg atactcaaaa aagctttgca actctgtcaa ttctttacga
     8101 atcgtacgtt ttccaacata tagtttttct gaaagttctt caagactaaa tccgtctttt
     8161 gctactagca gatggaaaat caataactga cgaagctgtt ttttatccat ttctgtttga
     8221 gtctcttcat taagcgctcg tttcagttgt tccctttcgt gaggcagacc attcaacaag
     8281 atacctgtac ctttttttct ttcaaacatt aaaggcagat gtttgtctct caaccaagtt
     8341 tcaatggtat tcaaatcgct atgaatcgtt ctttctgaca catggaaatg ctctgctagc
     8401 ttttttgttg ttatataatt attgttctca cttaattgat agatcaaaga ttgctgacgc
     8461 tgattcataa tgtgcagaaa ctcccttcta aataaaaaat cgatcatgat aacacatcta
     8521 ttatgtctca tcacggtcga tttttcatct tatgttacat ttcgcttaat aattggtgaa
     8581 ctgttgtttt atctggcgct tccatcagtg cttgacggaa gtcatcatcc atcaatttac
     8641 gtgatagacg ttgcagtatt ttcaagtgca tatcaccttg gctgttttca ggtacagcaa
     8701 tcataaagat catatgtgca ggttttccat ctaaagaatg ccaatctacc ccagattgac
     8761 ttctggcaaa tgccactgtt ggtttaagaa cataagctga ttttccatgt ggaatagcaa
     8821 tccccatacc gattcccgtt gtactttcat gttctctatc atagatgact tctttaaatc
     8881 tggttttatc tgtgatcgcc gctgtttttt ctaaacgttg gactaaatct tcgattactc
     8941 cgtcttttgt tgttgctgtt tcacctaaaa cgattgcttc atctgacata atatccgtga
     9001 tagaatcaaa agtttcagtt ttcgtataaa catcacttgt ttcagtctct gatagatctg
     9061 ctactgtcac accatttgct gcagcaaccg cagtttgttg taatgtcggt ttaaggattc
     9121 ttaacattag cacggttaca gcaataccag caatcactgc tacaaaaaac atggctacgt
     9181 gatccactgc tcctaaaacc gctacgatcg gaccgccatg agctacatga tctgtcactc
     9241 ctgaaagcat cgcgatcaca cttcctgtca ttgccccgat tacaatgctt ggaatcaccc
     9301 gtagtggatc tgttgaagca aacggaatag cgccttctgt aatcccaaac agtcccattg
     9361 ctaaggacgc cttaccagct tctcgttctg ttttcgtaaa tttggttttt aaaatcaatg
     9421 atgccagccc caatgctaat ggagggatac agatagctac agcaattggc cccataatgg
     9481 aataattgcc ttctgcaatc aagccagatc cgaacaaaaa ggctacttta ttgaacggcc
     9541 cccccatatc aaaagcaatc atcgctccta agatcatagc tagaacaatt tcacttcctc
     9601 cttgcatccc tgctaaaaag tgagtcaatg aactaaatag aagagctact ggttctccaa
     9661 taacatagat aaacacaaaa ccaactgcca atgatcctaa aattgggata acaataattg
     9721 gcatgatcga ttgtaaagct ttcggtactc gcactttctt gattcctaaa gcaatgaatc
     9781 ctgctaaaaa tccagtgatg atcccgccta agaatcccgc attcgcatca ctaccatata
     9841 atgctccgtt tgccgctaac atcccaccaa caaatccagg aacaagtcct ggacgatctg
     9901 caatactttg cgcaataaat gcagatagaa ttggaaccat caaccccata gcgatacttc
     9961 cgatactatt catcgttgcc caaatcgttc cttctggtat cttgatccct tcaggtgtag
    10021 ctgttcctcc taatgttaaa gaaatcgcga tcaataaccc tcctgtaact acgaaaggta
    10081 ccatgtacga aacgccattc atcaaactct tatagatttt tttacctaaa ttttctgatt
    10141 cacttttatt ttcggaagaa ataattgcct ccgtactttt atagactggc gcttttcctg
    10201 ctaaaatatc tctcaccaac tcatctgcat ggtggattcc ttcttgcaca cctacggtta
    10261 tcaaaggttt accgccgaat cgtgatttat ctattttagt atctgctgcg atgattacac
    10321 cagctgcttc ttcgatatct cttgcagtca attcattttc aataccgatg gaaccatgtg
    10381 tttcgacttt tacctcggcg ccaactgctt tccccgcttt ctctaagttt tctgctgcca
    10441 tgtatgtatg ggcaatacca actggacagg atgtgattgc aataattttt ttcattttcc
    10501 taccgccttt ctacatttat agtatataaa aacggtttct ttttattaag cacaaaatga
    10561 ttccgtaata actgaaattc ttggaataaa tagaaatttc gctaaaaaaa ttgatatacg
    10621 atttattatg ttgaataaaa taatattgga aaatcttaca tttctgtact tctgcttacg
    10681 tacatctaaa ttattatcta cattaacaat ttccgtaatt aattataaaa agcttattcc
    10741 atttaatttc attctggaat aagcttctta ttttctaatg agttagtgaa taattttttt
    10801 taccatgctt aatagtatct ttacgtctcc tatacgcgta tctcctgttt ctttatctat
    10861 aattgaacta taaacatgag gaatcacttt ttttacacct gcatcgattg caattttcag
    10921 aatttcttca aagtttgaaa gatcaattcc tcctgttggc tctaaataga attcatattt
    10981 tgcgcaagct tccgcaactg ctttaaattc ttccctatgt gctaagcctt tcatcgggaa
    11041 atacttgatt gaagtacctc ccatgtcttg cagcatttga atggcagttt caataggcac
    11101 ttcagctgct ggcgcttgtg aacttaacgg acctgtagca atattgacat agccgatctt
    11161 acctgttggt gaaaccaatc cattgatgac tgtatcattt tggcccaata aagcacgaga
    11221 agtccccacg ccagtaaata cttgattgac atgttgtggt tgtaaaaccc cagataaacg
    11281 cgaaaccatc tgactttggt ttggatctcc agcgcctaat ccaactgaca acgcattatt
    11341 ggttgcgtct tggtaccttt tcatatcttc gattgctgct tcatcgctag cataattttt
    11401 cgatagcaca cctagtacga catgcccttc cgctgcttca tagcatgctt ttgcattttc
    11461 aactgaatga gctaatacat ttaaacatac acgattttta taataatttg ggataactga
    11521 caatttaaaa gcctcctaca tgatttctct caatcttttt ataatttttt ccatttcgtt
    11581 ttggctgacg gaacgaatgt cgaactcaat gattccgtta tttgcttgat attctcgggt
    11641 gtagactgct ggatttcgtg cttttaattc ctgaatgact tctttcgcac ttttatcgcc
    11701 attaatcgtt acacttgctc gataaatatc tctgccggca ccatcttgaa ctattttcgc
    11761 tgaaaggtca ggaagttgat tgattgcgtt aacaaaaggc gctaatcgtt gcttcatgga
    11821 gtcaccggat tcgctcccat tttttaaata atcttctagt gcctgggtaa agcctaaaat
    11881 attatcttta ccgattttca ttgctcggcc aataccttta gactgtagcc gaatccattg
    11941 aatatacttc ttttttccta tgaccagtcc ggcactcgga ccttcgattg cttttgcccc
    12001 agagtaaatc actaagtcaa cgcccatttc gctatatttg aataaatctt cttccgcagc
    12061 tgcatcaacg atcaatggca aacgatgagc tttggcaact tctaccattt ctgccaccgt
    12121 cagcatactt ttctgaactg catgatgact tttaatatat agaagcgcag cagttttttc
    12181 cgtaatcatc atggaaacat gttccggtgt acacatattt gcatagccag cttctatgac
    12241 ttttcctcca ccttgagcaa ccattacttc aaccggtgtc ccataatcta cattatgtcc
    12301 cttaggtatg atgatttccc gtctagcgat tttttctgta tagggatgat agacatggta
    12361 catgtcgccc tgtccgatta aagcagcaac agattgagca atccctgctg aagcagaaga
    12421 aacgatttgc gcatcttcca ctttcagtaa ttgagaaaga taagcgccgg ttttgatggc
    12481 aagatcactc atctcaaaaa aatgctcgcc tccaaatcgt tgtgctgcta ggacactttc
    12541 agatacctta gaaaccccta ggatcgtcat ttttcccgat gcattaatga cttcttttaa
    12601 atcaaacttt ccatagctaa ttgtcatata tgacgcctcc aataattgtt ttaatcggta
    12661 aaatttgttc tttagctata cgtgtaaatc cgttggaatc tttcaattcc ttttcatttt
    12721 caacaatctc aaatatcgtc aaatctgcat cataattgac agctaactgg cccttattct
    12781 tcaaatgaaa attccgtgca ggaactgctg taactttttc aatgatttct tcccaagaat
    12841 agccgactac cctcaatttt tctaaagtag ttgctagatc aaaaactggg ccatttgtac
    12901 gattgcggat gtagatatcc gtactaatag aggtagcttt cattcctgct tttagtgcgg
    12961 tttctgccac atggaaatta aagctatccg tcccatgtcc aatatcaaag acgacaccat
    13021 tattgtaagc atcccagaca aagtttttaa tttgatctgt ctttggatct aaaataccgt
    13081 ttggttttcc attaaaacaa tgggtcaata catcgcctct ttccattctt gacaatactt
    13141 cttctaactc aggaggtgca gaaccgatat gaaccatcaa tggcagatca ttgttttctg
    13201 tctggatttt tttagctaat tctaatggct taatgccatt ttcaccaaca actgtcttac
    13261 tcattctcgc tttaatcccg acaataaatt ccggcagttc tgctaacgca gtacttacta
    13321 gatcttcttg aatctttgtt agatcagcaa gttcatcttg tttgacaatg ccccatttag
    13381 agatattcat tagcgcatac acatttgtct tgaccgattt cgtcaattga taaaattcac
    13441 ggatgttttc tgctcccgta gttccagcat caatgaccgt cgtcacacct ttttcgaccc
    13501 ctatctgatc aggatagtca taataaagat tcatcttttc atagcagtgg acatgatcat
    13561 cgatccaccc agcggataaa taatactttc cctccaagtc aactacttct tgcgccggct
    13621 gatttatttc tgctgcaact gctttaatca tgcctttttc tatagcgacc tctattttag
    13681 atccatcaac taaccttcca tttttaatca aagtatcaaa catttaatga ccctctttct
    13741 attaatttac tgccgggaag attgctccta aaatcattgc tcctaaaata gcacctccag
    13801 caattggttt tttccaagca tagaatccta gtgctcctaa tgtagcgcca atgccgattg
    13861 gaatagatgc tgcacacgcg cttaatataa ttaatggacc caaaaaacgt ccagatgaat
    13921 ttcctgcccc catcatgacg tcagcaccaa aagtggaatt tgattgattg atcgtaaatt
    13981 tacgaatcaa gacaatgatg ccaccaacaa tcaaacctag aaccaaacca gtcaataaag
    14041 agagtgcaaa attatcgatt ggtgccgcta ttttcatact taagagaatt gctggtaccc
    14101 caattccgat cccagttagg atcgaaccgc ctaagtccaa gatcccaact aaagagcctt
    14161 ctaaaattct ggcaaataag aaactagcac caaacgctgc cgctgcgcca taggatccgc
    14221 catccagccc ggcttttaac atcgccacga tcgaaacttc attgaatgca cccacaccat
    14281 acatcacata catgtgtgtt cctgcaaata cagcagcgga cataattcct acgattagcg
    14341 gaaaagacca gtctgcaaac caaaagctct tttgccgctt tgcttctaat acttttgctt
    14401 gttcttctgt tgtctccatg attctcctcc tatttcacac taaagatgtt gtgcaagtta
    14461 tcaagccaat taggcacttg aagctggaag ctcttgatca tttccatatc aaatccacgg
    14521 aaaaatccac tgaaaataaa taataaaaca atagcgatca tcatgctctt cgtgacgcga
    14581 ttccaaccgc tctcatcgac gcctttacca atcaagattc ctagaacaac accaggtacc
    14641 gcatttccca taatcaattg cgcgatacca ccaaaaagag tgccccaaaa accagttcgt
    14701 ttccctgcgt ctaaagctgc caaccaaaaa ataactggca ttactgtatt gatcagaatc
    14761 gtcgctgctg gtaccaaaac ggaaaccgcc gtcacttgta acgcctctgg aattgctgca
    14821 gctgttgtgt ttaagaaggc taccaccact gccccgataa ccgctccaat gatccccatt
    14881 tttttcggat catgcatggt ctgggtgaca tctttgtttt ttgctagaag tgctgccgct
    14941 gcccaattgg gaatgattcg atgcgtcaca tcttgtgtga atgcaccagc accaactgaa
    15001 gaagcccatg cattaaagaa aaagcctaaa ccaaatgaaa aatgagaagc tggatcaccc
    15061 atgcaggcat tcatttcgcc taatgttcta aaagctccta aaccttgtgt attaggtgca
    15121 tgaaacattc gagctgcccc gacagatgct gcaaaaccaa gtaatccccc gataatgacc
    15181 gatttgacta gaatgattac gaaatccacg ttattctttc ccccttatga ttcaaaatta
    15241 gattttttta gttacaaatg gaatggcaat cccatttgga tcttcagtca tttgctggct
    15301 gaacgatatg tctgccaatt ttatttctgt tacagccaca gtaacagaga gttcgacttc
    15361 atagctaata cgttgccgtg ggaagaagaa aaatagaaag cgttctgtgt atttgttttc
    15421 gaccgccttt aaaatatcca cattgactgg ttcgattcgc aataatacgt ttgattgttc
    15481 cattattttg ttttgaattt ctgctaacgc tgatgcaaaa gcatgttgct ttgtttcccc
    15541 tttaccggag acgataactt tttgctgaat tgtcttttga acgctcattt catgtccctc
    15601 tttctatttt ccatgctttt tgataaattc ttctgtcaga cgtttcccta attcctcttg
    15661 atccataaag ccgaaaccta gaacagtttt gccatccttg atagctgtga tcccttcttc
    15721 aatcgaacgc ataccatgtc gttctggata gccatatttt gttgctgctg ttaatgcacc
    15781 tgcaccacca ctaccacaaa aagagatacc catatctgca tgctcttgtt gcatcacatc
    15841 gcccaaacgc atatctgccc ccattcctgg tacgacaact gcttttccac cagctgcttc
    15901 tacccctttg gccacatttt gtcctttacc catacggtct gcaataacta ctttaatcat
    15961 ga
//
LOCUS       DACGLE010000064        15497 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0534
            SAMEA3304449-rid9560453.denovo.068, whole genome shotgun sequence.
ACCESSION   DACGLE010000064 DACGLE010000000
VERSION     DACGLE010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304449
            Sequence Read Archive: ERR985064
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15497)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15497)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702225.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702225.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 20:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,045
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 27 of 67
            Pseudo Genes (incomplete)         :: 25 of 67
            Pseudo Genes (internal stop)      :: 19 of 67
            Pseudo Genes (multiple problems)  :: 4 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15497
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304449-rid9560453.denovo.068"
                     /strain="VREN0534"
                     /db_xref="taxon:1352"
     gene            100..993
                     /locus_tag="IXT20_002105"
     CDS             100..993
                     /locus_tag="IXT20_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1803 domain-containing protein"
                     /protein_id="HAQ4860541.1"
                     /translation="MNYYSKNKENFYQKLTDDPLFSSLTDYLYEHREQETILRELKKE
                     FSQNKFSHFLDLLIDAGLIKREERRYHLNFPIFDPKDYLQQATSAAETIAEQLKRLSV
                     DEQKLAMGEVIWAYCFEDERKEAYFYGVRNSRETELLRTTAGNEKYRFITLSSIEHFP
                     LTLANYFFVQKNQLPVTKAFKELAELIGDVNESYFFDQIEVIVDRIRKNKYKNRRPSI
                     FHQSLLVTNTIKEEESFTLELPIVEKNNFEIELPTLDPSLTMEEMAFLKRQIFSELSK
                     KFIPHAFSYIKEYGTVLVSKT"
     gene            1127..1252
                     /locus_tag="IXT20_002106"
     CDS             1127..1252
                     /locus_tag="IXT20_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ4860542.1"
                     /translation="MQKNGEKCGMTKEVVIRKVRFLNNQYYDSVKYGILWEELAD"
     gene            complement(1386..2018)
                     /locus_tag="IXT20_002107"
     CDS             complement(1386..2018)
                     /locus_tag="IXT20_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4860543.1"
                     /translation="MEIQRRTTNFLHLGSFFATLTILFFLCYSYLKTSHAPSSALGWL
                     LLDHSDITEAEENPFFLILSSLCGLLFSVYFGAYFFHRHPGTLNEASSVLIAFKTFSF
                     LAILFYSFNHNTLFFFIMNGLVVILSIFTYFIFSLIFSQLKCPLFFRLVPVTCFIYSL
                     FSFFVLALMPLTNPSTIQVAGNLLDMLFIVSLGVFMWQRKNKQNKKHFEA"
     gene            complement(2166..2708)
                     /locus_tag="IXT20_002108"
     CDS             complement(2166..2708)
                     /locus_tag="IXT20_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2179 domain-containing protein"
                     /protein_id="HAQ4860544.1"
                     /translation="MIDPKMLGMIFLINFAYITLNTLRFMLTMKGYRLIAPLVSMVEI
                     TIYIVGLSLVLDRLDNPLNLFVYALGYAVGISVGILIEDKLALGYIMVTTILPANSAE
                     DKSLPGILREHGYGVTQTSAMGLEGERLVLEILSPRKSERELYRLIKEIEERAFIISY
                     EPKYISGGFWTKKVRKRQGK"
     gene            2843..3232
                     /locus_tag="IXT20_002109"
     CDS             2843..3232
                     /locus_tag="IXT20_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4860545.1"
                     /translation="MKTVNRVFCASHVLSLLLSIYLGVYSIQQPIEVENIVFQMSLTD
                     GLILSVFFFLILFIGNIFGSVASFLNFSIYPLLSLALGVVGLCSLCLFPEPFSPAFIL
                     FGTLNLFQTTVGAWLLWRSGNLMKIGE"
     gene            3236..4000
                     /locus_tag="IXT20_002110"
     CDS             3236..4000
                     /locus_tag="IXT20_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HAQ4860546.1"
                     /translation="MNPIILKRSVHHIPVLEVVLKELREERIPVVIYYHGWQTSKELV
                     LTQGRKIAQQGIRVILPDAMNHGERKQPVSSIPSYTFWDSIYGNLFEFDTLIKHLEKR
                     GLLHDQLAVGGVSMGGMTTCALLAKHPHIVAGVCLMGSPAPLEYGKEIVRRASEYNYV
                     VPKDYFDLISWVKSYDLSLQPEKLAGRPLFFWHGQADEKIPYIQTERFVEKNRHKSYG
                     KEIVFRSSKTQRHMVQIPLMEEASTFLAKNLIKKYK"
     gene            4129..5076
                     /locus_tag="IXT20_002111"
     CDS             4129..5076
                     /locus_tag="IXT20_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAQ4860547.1"
                     /translation="MPTLADVAKRANVSKMTVSRVINHPEQVTDELKELVFSAMAELD
                     YRPNIAAKALVTNRSQIIKLFILEEIDTTEPYYMNLLMGIAKRIGKAHYSLQLVTNDA
                     FDVGSCDGYIITGVRKKDFDWIDRLEKPVVLFGENSEGYDYVDSDNEYGTAKATQYAL
                     ERDYQTIIYIGIEVDEPFEISRETGYLSVMKEVDLPSKILRFPNHSTEVERYMTENWA
                     DFTDKTCFVCSSDRLALGAVRGIINAGGHLPEEFGVIGFDGVFLDQISSPKLTTIKQD
                     IIRMGEVCGEMLLKKIEEKGASQGYRRFFPELVIRETTN"
     gene            5206..5946
                     /locus_tag="IXT20_002112"
     CDS             5206..5946
                     /locus_tag="IXT20_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4860548.1"
                     /translation="MKEIRRKHFRFPAYDDELGVKLTNDKKRVLFDGQEDLLTEQNQS
                     PFEQMKEFHFTNTNTEKIRRKRRATNGLKSAPRKENLEKHKGKLPDYTGHYQSEITST
                     GRKKLFGDSSRSVRQSETRTESKHTTRNASVDTKVRERSYFVPKYVPASIIPDPRESK
                     ISEDHLIESMKKTDDSYLLFDTEPTPYQEKRNGEPSVRTFNRTKTLEETKQSEEKKSK
                     KSHGILERSLQGLIDDQGNSLGENSYFK"
     gene            complement(6237..6905)
                     /locus_tag="IXT20_002113"
     CDS             complement(6237..6905)
                     /locus_tag="IXT20_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAQ4860549.1"
                     /translation="MKKLNLFTELCGIILGAGIYGLAVTGINIPSKLADGGVTGIALL
                     LHNLFGFAPSVTSLLFNLPLLLISLFLFGKHAFLRTIVGTFALVFFLHLWESLHIHFA
                     VDSLLINSLMTGILSGIGCGLVFRFGGSTGGTDIIYQAIEKYFHIKIGKSLFLITFGI
                     LVVSLLYLDLTHFLYTLLSCSILSYTLNKVKYLEIGSVLPQLEAKQTTETFEPLDPLE
                     QLEH"
     gene            7039..7326
                     /locus_tag="IXT20_002114"
     CDS             7039..7326
                     /locus_tag="IXT20_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4860550.1"
                     /translation="MRAADLLTILTKSPKKTEYQGVFLAQEQKLVPVTMVQINKANQF
                     IFLFEHGKAPLAMKDIFLFLMKNREKAVYYQSKEELIPLYGVKEIENKVVI"
     gene            7733..9271
                     /locus_tag="IXT20_002115"
     CDS             7733..9271
                     /locus_tag="IXT20_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /protein_id="HAQ4860551.1"
                     /translation="MNNFIQTLSERKGELLEAAFQHLSISLISLLIAALIAIPLAIWA
                     ANHKKMAEVLLQITGVLQTIPSLALLGLLIPFVGIGTVPALIALVIYALLPIFQNTYI
                     GLAEIDPSIEEAAVAFGMSRMRRLLKVELPIALPVIISGIRTALVLIIGTATLAALIG
                     AGGLGTFILLGIDRNTPVLTLIGAISSALLAIIFSGLIRWLQHKKPRYSIITLAAVVC
                     LFGGVSLYQSALFKNETITIAGKLGAEPEILIQMYKELIEDNTKTEVELKPNFGKTSF
                     LFNALDQGQIDIYPEFTGTVLESLVKVPEAMKSADLTKEKTYDEAEELLNKQFDMTLL
                     KPMAYQNTYALAVKRSFANEHQLKKISDLAAISDQIKAGFTLEFIDRSDGYKGIQEAY
                     GLNFSSVQSMEPSLRYQAINNGDVNIVDAYSTDSQLKEYDLVTLEDDRGLFPAYQGAP
                     LMKKDFAKEHPEIVKALNKLSGKITEDQMIDMNYQVNVEKKKPTEVARIFLKNEGLVK
                     EEDQ"
     gene            9268..10233
                     /locus_tag="IXT20_002116"
     CDS             9268..10233
                     /locus_tag="IXT20_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ4860552.1"
                     /translation="MTKLIEFKHVQKKYDGKYVIDDLNLAIKKGEIFVLVGPSGSGKT
                     TTLKMINGLSKPSAGDIYFKGKSLNEYNLQKMRWNMGYVLQQIALFPTMTVKQNIEVI
                     PEMLGWEKQKRADRVDELLQKVGLSPDIYRDRMPRELSGGEQQRIGIIRAIAASPDVI
                     LMDEPFSALDPISRNSLQELVLSLHEELGTTIVFVTHNMEEAIKLGDRIAFMKDGEII
                     QCDTPEQLLMNPKNDYVRHFFDEPKQTKEWRVEDLVVNGYFLNEIPENARQQVCFDTP
                     MKEVYSLLSVYPSIVIVEKQRSIGSLTSKEIFAFLSREEEGNENI"
     gene            10220..11542
                     /locus_tag="IXT20_002117"
     CDS             10220..11542
                     /locus_tag="IXT20_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAQ4860553.1"
                     /translation="MKTYDAIIIGSGVSGLSAAYGLKEAGKTVLVVEEDLWGGTCPNR
                     GCDPKKVLLSAVEARNRVKQLSGKGFNEIPTANWEELQKFKRTFTDPVPESRKKQLAE
                     AEIDHLSGTARFLDDSSIEVNEEVFHADYLVLATGQRPTILPVEGKEYLKTSADFLSL
                     PVLPKEIIFIGGGYIAFELATIANAAGSKVTIVHHNQRPLKEFEASLVEEAVHQMEAS
                     GIQFAFGVETQKIISEGTRYRLVGKETELVADMIFCATGRQPNTESLALEQANIVFDK
                     HGIAVNDYLQTSNPKIFACGDIVSRKTPKLTPVATFEGNYVAKRITDATSEPIKYPII
                     PTIVYASPKLAEVGVTKSHASSSDQVVEMDLTSWFTYHRVNEPVAKAELTFDQQNYLI
                     GAAVISEQADELIDDLTLVINQKLTKKELDSYIMGYPTLASDLSYLLK"
     regulatory      11605..11709
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            11783..12526
                     /locus_tag="IXT20_002118"
     CDS             11783..12526
                     /locus_tag="IXT20_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ4860554.1"
                     /translation="MKKNLYPAVSMAFILIIWQIVVIVWKIPAFILPGPAAVFSALAA
                     DAQVLLSHSVTTLNEAVLGLVIASVLSFITALAMDYWKFLEMSFYPLLVISQMLPIMV
                     LGPLLTLWFGFGMLPKVILVVLISYFPIVVSFSDTLRKVSKEQIIFLKTMGADTGKIY
                     RIYKIPAGLSGFFSGLKVAATYCVSGAVVGEWLSAQAGLGYYMIRVKNSYQIDKVFAA
                     IICVILLSLLLNGACSLLKKGYYRMLYIF"
     gene            12541..13533
                     /locus_tag="IXT20_002119"
     CDS             12541..13533
                     /locus_tag="IXT20_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAQ4860555.1"
                     /translation="MKKLWLLLPLLLLLSACGTAKETSSKQEIKDLKKVTLVLDYVPN
                     TNHTGIYLAKEKGYYKEAGLNVQIIEPGDNSTSIGLVGADKAQFGVSYQEDVTYAHAD
                     GQNIPVKAIATVIKHNTSGFATLSDSNIHSPKDFEGKTYAGWQSPSEEAVLKAVMEKD
                     GGDFSKLTMVGSNGEGPESLGKSSDIQWYFEGWDMIKAKEAGIELNYIPLKELDERLD
                     YYTPVIITNDQLIKSDPELVQSFMDATKKGYQEAIKDPNDSAKLLQKYAKENDRTFLE
                     ESQAFLSKNYTDDPENWGLMEEKVWRNYTAFMQENGLIKQDVPSQELFTNQFIK"
     gene            13545..14282
                     /locus_tag="IXT20_002120"
     CDS             13545..14282
                     /locus_tag="IXT20_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ4860556.1"
                     /translation="MSIQISHLNVVIENLKILEDIHFTIPKDTFTSIVAPSGAGKSTL
                     LKTLTGVQPMTSGTIKVDDQKVTGLHTAFSYMPQEDMLLPWLSVYQNVTLYQKINHLT
                     INEEQVRDYLDIFGLNGYEHFLPEQLSGGMKQRTALLRTIMNPSSYLLLDEPFGALDA
                     MTRGQMQDWMLKLPKKAKRTTLLVTHDIEEAIYLSDRILVLSARPAHVIAEIQVPEKR
                     RSREWLLQQSELKQTIYQLLAGELDVK"
     gene            14272..14994
                     /locus_tag="IXT20_002121"
     CDS             14272..14994
                     /locus_tag="IXT20_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HAQ4860557.1"
                     /translation="MLSDAHCHLDGSQSLALLQQEHTILTIINCDSPEEWKENRQLAA
                     SKTQALSYGIHPWKADSYTFEQVEPFLKKARIIGEIGLDNIWTNVPMTTQKKVFERQL
                     AFAAINEKPVVLHTKGCEKEILNYIQAYPNHYLIHWYSSLDYQQDYIDLGCYFSIGLD
                     LKKNPAVWQLAKAVPIDHLLIETDGLHAAEWALGGIFSEKDYVSLLEEQLKTIAEIKE
                     LSVEEVKKNTYQNLERFIRLGK"
     gene            complement(15078..>15497)
                     /locus_tag="IXT20_002122"
     CDS             complement(15078..>15497)
                     /locus_tag="IXT20_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HAQ4860558.1"
                     /translation="YSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEQPVVQETVQTEK
                     QAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTATNGRYIGRYQLDSSYL
                     NGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
BASE COUNT         5228 a         2602 c         3171 g         4496 t
ORIGIN      
        1 aaatggagga gaataacagt caattatgac tcttgttctt cctccttttt acgtaaaagc
       61 cttttaagta aaataaaacg gatagaaaag agaacatata tgaactatta ttcaaaaaat
      121 aaggaaaact tttatcaaaa actgaccgat gatccgcttt tttcttcatt gaccgattat
      181 ctatatgaac atcgagaaca agaaacgatt ctaagagaat tgaaaaaaga attttcgcaa
      241 aataaatttt cccattttct tgatttattg atcgatgcag gattaatcaa gagggaagaa
      301 cgaagatatc atttgaactt tccaatattt gatcctaaag attacttgca acaagccact
      361 tcggctgctg aaaccatagc tgaacaattg aagagactct ctgttgatga acaaaaatta
      421 gcgatgggag aagttatttg ggcgtattgt ttcgaagatg aacggaaaga agcgtatttt
      481 tatggagtaa gaaacagccg agagacagaa ttgctgagga caactgcagg aaatgagaag
      541 taccgtttta tcacactttc ctctatagaa cacttcccgc ttactttggc taattacttt
      601 tttgtacaga agaatcagct tcctgtaacg aaggcattca aagaattagc cgaacttatt
      661 ggagacgtaa atgaatcgta cttttttgat caaatagaag ttatcgttga caggatacgt
      721 aagaataaat ataaaaatcg ccggcctagt attttccatc agtctctact tgtgacgaat
      781 actataaagg aagaagaatc attcacatta gaattaccta ttgtcgaaaa aaacaatttt
      841 gagattgaat taccaacatt ggatccttca ttgacaatgg aggaaatggc ctttcttaaa
      901 cgacaaatct tttctgagct gagcaagaaa tttataccgc atgcattttc ttatatcaaa
      961 gaatatggaa cagttttagt gagtaaaacc taagagagca gtaagcaaaa attatactga
     1021 ctcttgcagg aggagccttt ctcttccttt atggttaaaa gaattatttg agtattttgc
     1081 ctatctacca cctattagat tcactacatg gtctggaaac aaaggaatgc aaaaaaatgg
     1141 tgagaaatgt ggaatgacaa aggaagtagt gataagaaaa gttagatttt taaataatca
     1201 atattatgat tcagtcaagt atggcatttt atgggaagaa ctagctgatt agattaggaa
     1261 gtccatttga taatccagaa tcactaagaa acgaatggcg agtcattaat aatttatcca
     1321 atcttgggtt attcttttag attgaagtgt ttaaaactaa aaaagcattt atgaagtcgt
     1381 aatagtcagg cttcaaaatg ctttttattt tgtttgtttt ttctttgcca cataaataca
     1441 cctagactga cgatgaagag catatccaac agatttcctg cgacttggat cgtcgaaggg
     1501 ttggtcagtg gcattaaagc aaggacaaaa aaggaaaaca agctgtaaat aaaacaggtg
     1561 acaggaacta atcgaaagaa gagcggacat ttcaattggc taaaaatgag tgaaaaaata
     1621 aaataagtaa aaatggataa aatcacgact aaaccattca tgataaagaa gaaaagcgta
     1681 ttgtgattaa acgaataaaa aagaattgct aaaaaagaaa acgttttaaa tgcaatcaat
     1741 acgcttgaag cttcgtttaa cgtaccagga tgtcgatgaa aaaaataggc accgaaataa
     1801 acagaaaaca gtaagccgca aagagaagac aaaatcaaaa aaaacggatt ttcttcggct
     1861 tctgtgatat cagaatgatc caaaagcaac cagcctaatg cactagaagg cgcatgtgag
     1921 gtcttaaggt atgaataaca tagaaaaaat aagatcgtca aagttgcaaa aaaagagcct
     1981 aaatgtaaaa aattagtggt tctacgttga atttccataa tatctccaaa ttaaattgat
     2041 aagtatataa agtataataa caaattgatg agtatatgtc gatgtttttt aatcaaaata
     2101 ataaataaaa actgtctaac atttaaaatt taatttagat attagacagt ttttaagttt
     2161 gtgagttatt ttccttgtcg ttttctcact tttttagtcc agaaaccacc tgaaatatat
     2221 tttggctcat aagaaatgat aaaagcacgc tcttcgattt ctttgatcag gcgatacagt
     2281 tcacgttcgc tttttctagg agaaagtatt tcgagaacta gccgctcacc ttccagcccc
     2341 attgcagaag tctgtgtcac accataaccg tgttctctca gtatacccgg caaactttta
     2401 tcttctgcgc tatttgctgg caaaatcgtt gtgaccatga tgtatcctaa tgccaatttg
     2461 tcttcaatca aaattccgac actgatacct actgcgtaac ctaacgcata gacaaataaa
     2521 ttcagtggat tatctaaacg atctaaaaca agggataagc cgacgatata aatcgtgatc
     2581 tcgaccatac tgacaagtgg agcaatcagt cgatagcctt tcattgtcaa catgaaacgt
     2641 aacgtgttca gagtgatata agcaaaatta atgaggaaga tcatccccaa catttttgga
     2701 tcaatcattt gtaaaactcc tttcatttat ataacaacaa tatcaaaaaa aatcgtaagt
     2761 gaagaaaaat tgaaacttta tacatacaaa aaaatgataa aatgattata atagaattat
     2821 aaagataggt taggtgatta aaatgaaaac ggtcaatcgt gtgttttgtg ccagccacgt
     2881 gctaagtctc cttctcagta tatacttagg tgtctattcg attcaacagc caattgaggt
     2941 agagaacatt gtttttcaga tgtcgctgac agatggttta attttaagcg tttttttctt
     3001 tttgatactg tttattggta atattttcgg tagcgttgcc agttttttga atttttctat
     3061 ttatccactc ctttcgctag cattaggagt ggtgggactt tgttctctgt gcttatttcc
     3121 cgagccattc agtcctgcat ttatattatt tggtacactg aacctgttcc agacaactgt
     3181 tggagcatgg ttgctatggc gatcaggaaa tttaatgaaa ataggtgagt aacaattgaa
     3241 tccaattata ttgaaacgtt ctgtccatca tattccagtg ttagaagttg ttctgaaaga
     3301 attaagagag gaacgtattc ccgttgtcat ctattatcac ggatggcaga caagtaagga
     3361 gttagttttg acgcaaggaa gaaaaattgc gcagcagggc attcgtgtga tcctgccaga
     3421 tgcgatgaat catggggagc gcaaacagcc ggtttcttct attccatcct atacattttg
     3481 ggatagtatc tacggaaatc tgtttgaatt tgacacactt atcaagcatt tagaaaaaag
     3541 aggtcttcta catgatcagc ttgcagttgg cggggtttcg atggggggaa tgacaacatg
     3601 cgccttgttg gctaaacatc ctcatatagt agcgggcgtg tgtttgatgg gttcccctgc
     3661 acctttggaa tatggaaaag aaatcgtaag gcgcgcatcg gaatataatt atgtcgtacc
     3721 aaaagactat tttgatttga tttcatgggt taagagctat gatttgtcat tgcagccgga
     3781 aaagttagct ggacgtcctc tgttcttttg gcatggacaa gctgacgaaa aaatccctta
     3841 tatacaaacg gaacgatttg tagaaaaaaa tcggcataag tcttacggaa aagaaattgt
     3901 ttttcgttcc tcaaaaacac agcgacatat ggtacaaata ccactaatgg aagaagcttc
     3961 cactttttta gcaaaaaatt tgataaaaaa gtataaataa ttgaaacgga aaatcactca
     4021 tgacgataga ggattttccg ttttttattt tgggctataa taaactctgt taggaaaatt
     4081 tggtataatt gatagtaaga atgacaacat gttagaaagg aagagagcat gcccacttta
     4141 gcagatgttg caaaacgtgc caatgtttca aaaatgacgg tctctcgagt catcaatcat
     4201 ccggaacagg tgactgacga attgaaagag ttagttttct cagcaatggc tgaactggat
     4261 taccgtccaa atattgctgc aaaagcactt gtcacgaatc ggtcgcagat catcaaatta
     4321 tttatactgg aagaaatcga cacaactgag ccctattaca tgaatttgct gatgggaatc
     4381 gctaaacgga tcggtaaagc gcattattct ctccaattag ttacaaatga tgccttcgac
     4441 gtcggttctt gtgatggata catcatcaca ggtgtcagaa aaaaagactt cgattggatc
     4501 gaccgtttgg aaaaaccagt tgtcttgttt ggggaaaatt ctgaaggata tgattatgta
     4561 gacagcgata atgaatatgg tacagcaaaa gcaacgcaat atgccttaga aagagattat
     4621 caaacgatta tttatatcgg tatcgaagtg gacgagcctt ttgaaatttc aagggaaact
     4681 ggttatttat ctgtgatgaa ggaagttgat cttcctagta aaatcttgcg gtttccaaat
     4741 cattcgacag aagtagaaag atatatgaca gaaaactggg cagattttac agataaaact
     4801 tgttttgtat gtagttcaga tcggctagca ctaggagcag taagagggat tataaatgct
     4861 ggaggacact tgcccgaaga atttggtgtg attggatttg atggggtatt tttagaccaa
     4921 atcagttcac ctaaattgac aacgatcaag caagatatta tacgtatggg tgaagtatgt
     4981 ggagaaatgt tgttaaaaaa aatagaagaa aaaggggcga gtcaaggtta tcgtcgtttt
     5041 tttcctgaac tagttatcag agagacaacg aattaagtct tattactata tatatattta
     5101 tgaaattcat cgctattttc ttaaaataat cagcgcattg ctactgaaaa caatgaatct
     5161 gtgctattat tgagaaaagt tccatttttg agaaggatgg tttgaatgaa agaaatcaga
     5221 cgaaaacatt tccgttttcc tgcgtacgat gatgagttag gcgtaaaatt aaccaacgac
     5281 aagaagcgag tattatttga cggacaagaa gatcttttga ctgaacagaa tcagtccccg
     5341 tttgaacaga tgaaagaatt tcatttcacg aacacgaaca ccgagaaaat ccgtcgcaag
     5401 cgtcgtgcaa caaatggatt gaagtcagct ccgcgtaagg aaaatttgga aaagcataaa
     5461 ggaaaattac cggattatac cggccattac caatcagaaa tcacatcgac aggacgaaaa
     5521 aaactttttg gtgattcctc tcgttctgtc agacaaagtg aaacaaggac agagtccaag
     5581 catactacac gaaacgcatc tgttgacacg aaggtgcggg agcgttctta ttttgttcct
     5641 aaatatgtac ccgcatcgat tatcccagac cctagagaaa gcaaaatttc tgaggatcat
     5701 ttaatcgaat cgatgaaaaa aacggatgac tcttatctgt tgttcgatac agaaccgacg
     5761 ccgtatcaag aaaaaagaaa tggtgagccg tctgtacgaa cattcaatcg aacaaaaacg
     5821 ttagaggaaa caaagcagtc agaagaaaaa aaatcgaaaa aaagccatgg tatcttagaa
     5881 cgctcattac aaggattgat cgatgatcaa ggaaactctt tgggagaaaa tagctatttt
     5941 aaataagtga tatacaaatt tatattcggc tacagttgga aaggaaacgc tgcggcgttt
     6001 ccttatttta tactctatcc gaaagtttat atcgtatcct caaatttttt tgagtttaag
     6061 cgaatttttt ctcccaataa gtaagaaaat aatggcaaaa taaaaaaaca aactattttc
     6121 gcagatcgtt tgcaaaaata gtttattttt ttataaagaa gattttactg aataatatca
     6181 ttttagttta cataatgata tttctgtaaa ttaaataaag tagtgaaaaa taagaatcag
     6241 tgctctaact gttcaagagg atcaagtggt tcaaatgttt ctgtagtctg ttttgcttcc
     6301 aattggggca aaacagatcc aatttccaga tacttcactt tattcaatgt ataagataga
     6361 atagaacagc taagtaatgt atacagaaaa tgtgtgagat ccaaataaag caaggagaca
     6421 actaaaatac caaaagtaat caagaaaaga cttttgccaa tcttaatatg aaaatatttt
     6481 tcaatggctt gataaatgat atctgttccg cctgtgcttc cgccgaatcg aaagacgaga
     6541 ccgcaaccaa tgccagaaag aatacctgtc atcaaactat tgatcagtag actatctaca
     6601 gcaaaatgta tatgaaggct ctcccataaa tgtaaaaaga aaactagcgc aaatgttccg
     6661 acgatcgtcc ggagaaaagc gtgttttcca aaaagaaaca atgagataag caataaaggt
     6721 aaattaaaaa gcagtgaagt gacagaagga gcgaatccaa aaagattatg caaaagcaaa
     6781 gcaataccag taaccccacc atctgccagc ttagatggaa tattgattcc tgtcacagct
     6841 aatccgtaga ttcctgcgcc taaaatgatt ccgcacaatt ctgtaaataa atttaatttt
     6901 ttcatttcta gcctcttttc ttttgaaaat tttcagcaac ttctttatta tactgactta
     6961 tactcttact tcaagaggaa aatgaaaaaa aattattttt ggctgtttct ttttatttat
     7021 caaataagga gtggaaaaat gcgagcagca gacttattga caattcttac aaaatcaccc
     7081 aaaaaaacag agtatcaagg agtttttttg gctcaagaac aaaaactggt gcctgtgaca
     7141 atggtccaga taaataaagc aaatcaattt atctttttat ttgaacacgg caaagcacca
     7201 cttgctatga aagacatctt tctttttttg atgaagaacc gtgagaaagc tgtttattat
     7261 cagtcaaaag aagaactcat tcctctttat ggagtgaaag agatagaaaa taaagtggtg
     7321 atataagtaa aaataagaaa acaagacgtt tatgatgtca aaacaaccat tttttacatt
     7381 tttggcaaaa aagtgtcatg atacagtata atattcgttg aacaggccaa taaaggtgtt
     7441 ctcccccacg agatttctac tttattggct gtttcttttt attaaaataa aagttgtgtg
     7501 tgtaaaaaag atatagctga ttccaagtga caatttcgta tagattttta tggtattcgt
     7561 agtgaaaaaa cgagaaccaa aaagttgatc cgactttgtt gcttggattc tttttttgtc
     7621 agcatattta gaggtatttt tttgttcgtg ctgtaaggtt aaggcaagac aagttagttg
     7681 ggaaaaagga aagaaatgtt tatacttaaa gaataacaga aaggaaggtc gcatgaataa
     7741 ttttatacaa acattaagtg aaagaaaagg cgagttgctg gaagctgcat ttcaacatct
     7801 gtccatttct ctcatttctt tgctgattgc agcactgatt gctattcctt tagcgatttg
     7861 ggcagccaat cataaaaaaa tggcagaagt cttattgcag atcacgggtg tgctccaaac
     7921 gattccttca cttgccttat taggtttact tatcccattt gtagggatcg gaactgttcc
     7981 agcattgatt gcacttgtta tttacgcttt gcttccgatt ttccaaaaca cgtatattgg
     8041 tctagctgaa attgacccgt ctattgaaga agcagcggta gcatttggta tgtccagaat
     8101 gagacgcttg ctgaaagtag agcttcccat cgcattacct gtcattatct caggtatccg
     8161 aactgcctta gttttaatca tcggtacggc tactttggca gcattgatcg gagcaggtgg
     8221 attagggaca tttattttgt taggtattga ccgaaataca cctgttttga cattgattgg
     8281 tgcgatcagt tctgccttgc ttgcaattat attcagcgga ctgattcgct ggttgcagca
     8341 taagaaacct cgatattcca tcattacttt agcagcagtg gtgtgtttat ttggcggcgt
     8401 ttctttatat cagtctgcct tgtttaaaaa tgaaaccatc acgattgctg gaaaattagg
     8461 agcggaacct gaaatcctga tccaaatgta taaagaattg attgaagaca acacaaaaac
     8521 agaagttgaa ttaaaaccga attttggcaa gacgagtttt ttgttcaatg cactggatca
     8581 aggtcaaatc gatatctatc ctgagttcac aggtactgtc ctagaaagct tggtgaaggt
     8641 acctgaagca atgaaaagtg ctgatttgac aaaagaaaaa acatatgacg aagcagaaga
     8701 attattgaat aagcaattcg atatgacatt attaaaacca atggcttacc aaaatacgta
     8761 tgccttggca gtcaagagat cttttgcgaa cgaacatcaa cttaaaaaga tttccgattt
     8821 ggcagctatc tctgatcaga tcaaagcggg attcacactg gaatttattg atcgttcaga
     8881 tggatataaa ggaatccaag aagcatatgg cttgaatttt tcaagtgtac aaagcatgga
     8941 gccaagtttg cgttaccaag caatcaataa tggagatgtc aatattgtgg atgcctattc
     9001 cacagatagc cagctcaagg agtacgatct agtgactcta gaagatgatc gagggttatt
     9061 cccagcttat caaggcgcac cgctgatgaa aaaagatttt gctaaagaac atcctgaaat
     9121 cgttaaagca ctgaataaat taagcggaaa aataacagag gatcaaatga ttgatatgaa
     9181 ttaccaagtc aatgtcgaaa agaaaaagcc gacagaagta gcacgaatct tcctgaaaaa
     9241 tgaaggatta gtaaaggagg aagatcagtg actaaattaa tcgagttcaa acatgtgcaa
     9301 aaaaagtatg atggaaagta tgtcatcgat gacctaaatc tggcaatcaa aaaaggagaa
     9361 atctttgttt tggttggtcc ttccggtagt ggaaaaacaa ctactttgaa aatgatcaat
     9421 ggattatcta aaccatcagc aggagacatc tacttcaaag gaaaatcgtt aaatgaatat
     9481 aatctgcaga aaatgcggtg gaatatggga tatgtgctgc agcaaatcgc tttgttcccg
     9541 accatgacag tcaagcaaaa tattgaagtc atccctgaaa tgttaggttg ggaaaagcag
     9601 aaaagagcag accgtgtaga tgaacttctt caaaaagttg gcttatctcc tgatatttat
     9661 cgagatcgta tgcctagaga gttgtctgga ggagaacagc aacggatcgg gatcatccga
     9721 gctattgctg caagcccaga tgtgattttg atggacgagc catttagcgc attagatccg
     9781 atttcaagaa actcattaca agaattggtt ttgtccttac acgaagaatt gggcacgacc
     9841 atcgttttcg ttacgcacaa tatggaagaa gcaatcaagc taggtgatcg tattgcattt
     9901 atgaaggatg gagaaattat ccaatgtgac acaccagagc agttattgat gaatcctaaa
     9961 aacgactacg ttcggcattt ctttgatgaa ccgaaacaga cgaaagagtg gcgagtagaa
    10021 gatttagtcg tcaatggata tttcttaaat gaaataccag aaaatgctcg tcaacaagtt
    10081 tgctttgata cgcctatgaa agaagtatat tcgcttcttt cggtgtatcc aagcattgtg
    10141 atagtagaaa agcaaagaag tattgggtcg ctgacaagta aagaaatctt tgctttttta
    10201 agtcgagagg aggaaggaaa tgaaaacata tgatgcaatc atcatcggtt caggtgtgag
    10261 cggcttaagt gctgcttatg gattgaaaga agcaggtaaa acagtactag tggtcgaaga
    10321 agatctatgg ggcgggactt gtcctaaccg tggatgtgat cctaaaaaag tcttgttaag
    10381 tgcagtagag gcacgtaatc gggtaaaaca attgagtgga aaaggtttta acgagatccc
    10441 aactgccaac tgggaagaac ttcaaaaatt caaacgaaca tttacggatc cagtcccaga
    10501 aagccgaaaa aaacaattag cagaagctga aatcgatcat ctttcaggaa ccgctcgttt
    10561 tttggatgat tcatctattg aagtcaacga agaagtcttc catgcggatt acttagtttt
    10621 ggcaaccggg caaagaccta cgatcttacc agtggaggga aaggaatact tgaaaactag
    10681 tgcagacttt ttatcattgc ctgtactgcc taaagaaatc atttttatcg gtggtggtta
    10741 tattgctttt gagctagcta cgattgccaa tgctgcaggg agcaaagtaa ccattgtcca
    10801 tcataatcaa cggccattaa aagaatttga ggcgtctttg gtagaggaag ccgtccatca
    10861 aatggaagca tctggcattc agtttgcttt tggtgttgaa acccaaaaga tcatttcaga
    10921 aggtactcgc tatcgtttag tcggaaaaga gacggaatta gtagcagata tgattttttg
    10981 tgcgactggg agacagccga atacagaatc tttagcactt gagcaagcga atatcgtctt
    11041 tgataaacac ggaattgcag tcaatgatta tctgcaaacg agcaatccaa aaatttttgc
    11101 gtgtggagat attgtttcaa ggaaaacccc aaaattgaca ccagtcgcca cttttgaagg
    11161 aaattatgta gctaagcgga taacggatgc aacaagcgaa cctatcaaat atccgattat
    11221 acctacgatc gtctacgcca gtcctaaact tgctgaagta ggagttacaa aaagtcacgc
    11281 ctcttcgtca gatcaagtgg tagagatgga tcttaccagt tggttcacct atcatcgtgt
    11341 caatgaacca gttgctaagg ctgaattgac ctttgaccag caaaattatt tgattggtgc
    11401 tgcagtgatt agcgagcaag cagacgaatt gatcgatgat ttgacactcg tcatcaatca
    11461 aaaattgact aaaaaagagt tggatagcta tatcatgggg tatccgacat tggcaagtga
    11521 tctctcctat ttattgaaat aatcagcatg ataactaaag cgcttgttgc tttttccttc
    11581 ttgtgctaca ctttaaaaaa tgaatacctg tttctgggga tgtcgtagac gacatgagag
    11641 taagttgtga acttagaccc attgaacctg tcagttaata ctggtgaagg gagaaacata
    11701 gcagacgctt gaagcgggtt actatgtcgt gcccatttca agcgtttttt gttattcata
    11761 ttagaagaaa ggaggtaaca aaatgaagaa aaatctttat ccagcagtca gtatggcatt
    11821 cattttaatt atttggcaga ttgttgtcat agtttggaaa atacctgcct ttattttacc
    11881 cggacctgct gctgtatttt ctgcattagc ggcagatgca caagtattac tttcccattc
    11941 tgtcacgaca ttaaatgaag cagtgcttgg tttggtaata gctagcgttc tttcttttat
    12001 tactgccttg gcaatggatt attggaagtt tttagaaatg tctttttatc cgttactagt
    12061 gatttcacaa atgttgccaa tcatggtgtt agggccatta cttaccctat ggtttggttt
    12121 cggtatgctt ccaaaagtga tccttgttgt cttgataagc tattttccga tcgtagtttc
    12181 tttttcggat acattgagga aagtatcaaa agagcaaatc atctttttga aaacgatggg
    12241 ggcagataca gggaagattt atcgaatata taaaatacca gcgggtttat ccggcttctt
    12301 ttccggttta aaagtggctg ctacttattg tgtcagcgga gcagtcgttg gagaatggct
    12361 aagcgctcaa gcaggcttag ggtattacat gatacgcgta aaaaacagct accagatcga
    12421 taaagttttt gctgcgatca tttgtgtcat tctattgagt ttattgctga atggcgcttg
    12481 ttcattattg aaaaaagggt attatcgtat gctttatatt ttttaagaga aggggatcaa
    12541 atgaaaaaat tatggctttt attaccgcta ctgttgcttt tatccgcttg tggcactgca
    12601 aaagaaacaa gtagcaaaca agaaataaag gatttgaaaa aagtgacact tgttttggat
    12661 tatgtaccga atactaatca tactggcatc tacctagcaa aagaaaaagg ctattataaa
    12721 gaggctggtt taaatgtcca aatcatcgaa ccaggagata attcgacaag tattggtcta
    12781 gtaggtgcgg acaaagcaca atttggtgta agttatcaag aagatgtcac ttatgctcac
    12841 gcagatggac agaatatacc agtcaaagca attgctacag tgatcaaaca taatacttct
    12901 ggattcgcca ccctatcaga cagtaacatc cattcaccaa aagattttga aggaaaaaca
    12961 tatgcaggtt ggcaaagtcc aagtgaagaa gctgtcttaa aagctgtgat ggaaaaagac
    13021 ggcggggact tttcaaaact gacaatggta ggaagcaatg gagaagggcc agaaagctta
    13081 ggaaaatcct cagatatcca atggtacttt gaaggttggg atatgatcaa agcaaaagaa
    13141 gcgggtattg aactaaacta tatcccattg aaagaattag atgaacggtt agattactat
    13201 actccggtaa ttattacgaa tgatcaactg atcaaaagtg atccagaatt ggtacagtct
    13261 tttatggatg ccacgaaaaa aggctatcag gaagctatta aggatccaaa tgacagtgct
    13321 aaactgttgc aaaaatatgc aaaagaaaat gatcggacgt ttttagaaga atcacaagca
    13381 ttcttgtcaa aaaattatac ggatgatcct gagaattggg gattgatgga agaaaaagtt
    13441 tggcgcaatt atacagcttt tatgcaggaa aacggattga tcaagcaaga tgtaccttcg
    13501 caagaactat tcactaatca gtttataaaa taggagtgat agatatgtca atccaaatca
    13561 gccacttgaa tgtagtcatt gaaaacctga aaatattaga agacatccat ttcactatcc
    13621 ctaaagatac ctttacatcg attgtcgcgc caagcggagc cggtaagtca acattgctga
    13681 aaactttaac aggagtccag cctatgactt ctggaacgat caaagtagac gatcaaaaag
    13741 taactggtct ccatacagca tttagttata tgccgcaaga agatatgtta cttccttggc
    13801 tgagcgtcta tcaaaacgtt acgttatatc aaaaaatcaa tcatctaaca atcaatgaag
    13861 aacaagtaag ggattatctg gatatctttg ggttaaacgg ctatgaacat tttttgccgg
    13921 aacaattatc tggcgggatg aagcagcgaa cggccttgtt acgaacaatc atgaatcctt
    13981 cttcttactt gcttcttgat gaaccatttg gagcgttaga tgctatgacc agagggcaga
    14041 tgcaagactg gatgttgaag ctgcctaaaa aagcaaagcg gactacttta cttgtgacgc
    14101 atgatatcga agaagcaatt tatttatcag acaggattct tgtgctttcc gcccgtccgg
    14161 cacatgtgat tgctgagatt caagtgcctg aaaaacgacg gtcacgtgaa tggctactgc
    14221 agcaatcaga gttgaagcaa acgatttatc agttattggc aggtgaatta gatgttaagt
    14281 gatgctcatt gtcatttaga tgggagtcag agtttagcac tccttcagca ggaacacacg
    14341 attttgacga tcatcaattg tgattcgcca gaagaatgga aggaaaacag acagcttgca
    14401 gcaagtaaga ctcaagcctt aagttacggt atccatcctt ggaaagcaga tagctatacg
    14461 tttgaacaag ttgagccttt tttgaaaaaa gcaaggatca tcggtgaaat agggttagat
    14521 aatatttgga cgaatgtacc gatgactaca caaaaaaaag tatttgagcg acagcttgct
    14581 tttgcagcca taaatgagaa acctgttgta ctgcacacaa aaggatgcga aaaagagatc
    14641 ttaaattata tccaagctta ccctaatcac tatttgatcc attggtattc ttctttggac
    14701 tatcaacaag actatattga tttaggctgt tatttcagca ttggtcttga tttgaaaaag
    14761 aatccagcag tctggcaact agctaaagca gtcccaatcg accacttact tattgaaacg
    14821 gatggactac atgcagcaga gtgggcttta ggggggattt tttcagaaaa agattatgtc
    14881 tcactgcttg aagaacagtt aaagacaatc gctgagatca aagagctatc agttgaagaa
    14941 gttaaaaaaa atacttatca aaacttggaa cgtttcatta gattaggtaa ataaaaaaga
    15001 gatgtgacac aactagtgtc gcacctcttt ttggctttct ttgtataaaa gtaaactgtt
    15061 tgattaataa atgaatctta gtaccaaccg tttgcttccc agaatgcttt cgccgcttcc
    15121 caagaaccat aacgtgaagt aacatattgt tctgctactc tttcttggtt tgcagcagag
    15181 tagtcaccat ttaagtaaga agaatctaat tggtaacgtc cgatgtaacg tccgttcgtt
    15241 gctgtgtaag aaccgcttga ttctttttgt gcgatccatt cttttgcaga gcttgtgctt
    15301 gctggagtag cagcagtttc tgttacagca ggagcaggtt gtgtttcagc aacaggcgct
    15361 gcttgttttt ccgtttgaac cgtttcttgt acaacaggtt gttcagcttg gactggtgct
    15421 tcttgaacgg ctgcttgagg ttcagcagct gcttgtgcag agccttctgt tgggattgtt
    15481 aattgttgtc ctgagta
//
LOCUS       DACGLE010000065        14377 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0534
            SAMEA3304449-rid9560453.denovo.069, whole genome shotgun sequence.
ACCESSION   DACGLE010000065 DACGLE010000000
VERSION     DACGLE010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304449
            Sequence Read Archive: ERR985064
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14377)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14377)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702225.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702225.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 20:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,045
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 27 of 67
            Pseudo Genes (incomplete)         :: 25 of 67
            Pseudo Genes (internal stop)      :: 19 of 67
            Pseudo Genes (multiple problems)  :: 4 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14377
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304449-rid9560453.denovo.069"
                     /strain="VREN0534"
                     /db_xref="taxon:1352"
     gene            133..2604
                     /locus_tag="IXT20_002123"
     CDS             133..2604
                     /locus_tag="IXT20_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HAQ4860559.1"
                     /translation="MKNKWINRSKKKKVTQRSSDKVIRLFPSYKEGLTADQVKERIEK
                     GAANNSVDPTFKTNQQIVLENIFTYFNLIFLILAILLCLVESYKNLTFLPVIIANTGI
                     AIFQEIRSKKILDELNVLNTAKVNVIRDGIEQTIDIEDLVIDDIVILETGYQIPADAE
                     VVEGKLQVNEALLTGEADEITKEIGDPLMSGSFIVSGKAYARLDKVGADSYISQLTVK
                     AKTMGEGEQSEMIASLNQLIKWIGIIIIPIGILLFSQSYFFNGNTIKESVVAMEAALI
                     GMIPEGLYLLTTIALALSATKLAKQRVLLHNMKSIETLARVNVLCVDKTGTITENKMS
                     VQKMIVSKKQEIISQSDQLEACIADYAKAMAGDNATMEAVKAYFTKTTDQSYHHVIPF
                     SSVQKFSSVALADKVYVLGAPEMVLRDQLPEYAEEFVSFAEKGYRVLVFGVYDGILEE
                     PVLTEAVTPLGYILIANPIRKEARATFDYFKKQQVAIKVISGDNPLTVSNVAVQAGIS
                     HADQYVDASKLSEEEYEAAVEKYTVFGRVKPEQKKIFVQLIKRNNTVAMTGDGVNDIL
                     AMKEADCSIAMASGNEAAMQASQVVLLDSDFSRMPEVVAEGRRVVNNIERSASLFLVK
                     NIFSFLLSLFSVIFALTYPLEPSQITLISLFTIGLPSFLLALEENKKRIRGKFIMNVM
                     EKAVPGGLTDMIVVGALVICGVTLNLNKTDVSTASTMLLIAVGFLVLYKICSPLNKFR
                     SQIILFCASGNFFSVIFLHKLFSITGISAVSLLLLSILFFSADSIFRHLTTLVEYLFE
                     RKNEKEQKPFSRVAKKFLSIFKREN"
     gene            complement(2707..4443)
                     /locus_tag="IXT20_002124"
     CDS             complement(2707..4443)
                     /locus_tag="IXT20_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HAQ4860560.1"
                     /translation="MKWKKIYERLFFLILLVSLFFGGWQLISHYFQQQIVEQQESYLT
                     KKAQLLIRQLDVENLQAAQNKTALEEFVHQSNERITLMDATGKILFDTNNETLNEQRN
                     SRPEIKAVLNGGNLGSALRKSTTLNEDLLYVALPVKKSGQLEAILRIAEPTSVFLPRT
                     ESFRRWVFFFFLSFFLVLAGMIYYLIYQKNQPLKTVLPVLKKMVQNPNQAEVIMQTPD
                     QWEELYQTINALSEQMSKMYRACTTTEEQLYTLLNELMIGVFLIDDADSRLLLLNPKM
                     QLHLNVVSYQPEQRYTEVIQEPKLIQLIHQVTPEHPLIHQEITLTEGRQTLDLSLRYF
                     TNSEGTGQILGVAYDLTKVRRLEKLQKDFVGNVSHELKTPVTSLIGFTETLLDGAKDD
                     PQTLTSFLEIMQKDAIRLDKLIREIIQLSKDGENSYEIQTIYTEPYFQQIVQNYQPII
                     EKKRLTIRLIGKNEPFTTKTDILYPIIKNLIENAVQYSKSDSEIIIRYQATDDLSFSV
                     QDFGIGIDIEDQERIFERFYRVDKARSRHSGGTGLGLAIVKDYVQLLNGTITVDSHLG
                     TGSTFTVTIPKM"
     gene            complement(4440..5144)
                     /locus_tag="IXT20_002125"
     CDS             complement(4440..5144)
                     /locus_tag="IXT20_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ4860561.1"
                     /translation="MKKILVVDDEPSIVTLLTFNLEKEGYKVTSATDGGEGLELALEQ
                     SFDFIILDVMLPTMDGMEITQRLRQEKNETPILMLTAKDDQVDRIIGLEIGADDYLTK
                     PFSPREVLARMKAIFRRIEPRKQSKDEAEPEYLSIGQITADLTNYQVTVDDHLIELTP
                     KEFELLVYFMKRKDRVIDRDTLLDRIWNFDFAGQSRIVDVHVSHLREKIERDPKHPKY
                     LVTIRGFGYKFQEPKR"
     gene            5275..5778
                     /locus_tag="IXT20_002126"
     CDS             5275..5778
                     /locus_tag="IXT20_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HAQ4860562.1"
                     /translation="MIGISACLGGLACRYDGQKKEVPELMELVETGEAVMVCPEVIGG
                     LPIPRDPAEIIGGNGFDVWKDQAKVWTTSGEDVTEAYKNGAIKAYQKLQEKHIDLVIM
                     KEKSPSCGTHSIYDGSFSGIKKNGAGVATAYFIQQKMTVLSDEEWLKTRGEQNGNRKD
                     QSNERAL"
     gene            5738..6076
                     /locus_tag="IXT20_002127"
     CDS             5738..6076
                     /locus_tag="IXT20_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HAQ4860563.1"
                     /translation="MEIEKTNRMNALFEFYSTLLTEKQMNYMELYYADDFSLGEIAEE
                     YEVSRQAVYDNIKRTSKILEDYEKKLHLFSNYIVREQVLENMTAYITDKYPEDKKLLE
                     YVQQIQAIEE"
     gene            6097..7515
                     /gene="ffh"
                     /locus_tag="IXT20_002128"
     CDS             6097..7515
                     /gene="ffh"
                     /locus_tag="IXT20_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HAQ4860564.1"
                     /translation="MAFESLTERLQQAMGKIRKKGKVSEADVKEMMREIRLALLEADV
                     NLQVVKDFTKRVRERAVGAEVLESLSPAQQIVKIVDEELTITLGSETAELNKSPKIPT
                     VIMMAGLQGAGKTTFTGKLANYLKKNENARPLLIAGDVYRPAAIDQLKVLGQQLDVPV
                     FDMGTEVSPVEIVRQGMELAKEKKNDYVLIDTAGRLHIDETLMDELKQIKELTQPNEI
                     LLVVDAMTGQDAVNVADSFNQQLGITGVVITKLDGDTRGGAALSIRSVTGAPIKFIGS
                     GEKLTDLEVFHPDRMASRILGMGDMLTLIEKAQQDYDEKKAEELAQKMRENSFDFNDF
                     IEQLDQVMGMGPLEDLIKMIPGMNQVPGIENVKVDPKDVERKKAMVYSMTPAERANPD
                     LLNPSRRRRIAAGSGNSVVEVNRMIKQFKESRKMMQQMSKGDMNIPGMDQMFGSGVKG
                     KLGKMAMNRMIKKNKKKKKKRK"
     gene            7627..8205
                     /locus_tag="IXT20_002129"
     CDS             7627..8205
                     /locus_tag="IXT20_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HAQ4860565.1"
                     /translation="MRCPWSEQTESMRQYHDTVWGVPEYDDQKLFRKLMLDINQAGLS
                     WQTILNKSEAFDEAYDRFDIKKVANYGETKISELMENKGIIRNRRKIEAAVQNAKAVL
                     KIQEEIGSFSEYLWSFSNGKIVDSSYHSQEEVPTTSPLSDELAKDLKKRGFKFIGSTT
                     IYAFLEAVGIINDHLDSCFRKEEVVRIGRKGE"
     gene            8209..8730
                     /locus_tag="IXT20_002130"
     CDS             8209..8730
                     /locus_tag="IXT20_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAQ4860566.1"
                     /translation="MRKIIFYGAISLDGYLATKTDDLQWLFDTPTGKQTTYEAFYETI
                     DTTIMGRKTYQEAKKYLETEAIYPEKKNFVFSTHAQLQLPDAEVVNEDPVAFLKNLQM
                     TEGKDIWVVGGGRLIKPLLEHQMIDEWYIQITPVLLGDGIPLFQKGTYEARFEMLDTT
                     RFGEFIELHYVRK"
     gene            complement(8809..9363)
                     /locus_tag="IXT20_002131"
     CDS             complement(8809..9363)
                     /locus_tag="IXT20_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HAQ4860567.1"
                     /translation="MTKKIGFFIGSLRKDSYNKKVAEAFAKLLPEGYESVFVKIDDLP
                     FYNEDLETPEQAPAEWTRFREEVRGLDGVVFVTPEYNRSVPAVLKNALDVGSRPYGHS
                     VWDHKPGLVVTASPGGIGGFGANHHLRQSLVFLNVPTLQQPEAYIGNVANLIDENGNI
                     VEGTLSFFQTILDAYLDFSNKLTE"
     gene            9616..9891
                     /gene="rpsP"
                     /locus_tag="IXT20_002132"
     CDS             9616..9891
                     /gene="rpsP"
                     /locus_tag="IXT20_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HAQ4860568.1"
                     /translation="MAVKIRLKRMGSKKSPFYRIVVADSRSPRDGRFIETVGTYNPLK
                     DPAEVVLKEDLVLDWLSKGAQPSDTVRNILSKEGVMQKHHEAKFSKK"
     gene            9903..10154
                     /locus_tag="IXT20_002133"
     CDS             9903..10154
                     /locus_tag="IXT20_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HAQ4860569.1"
                     /translation="MKDLSDLVLTIVRPLVTYPDQVKLDVVESKDFYEYNLTVAPEDI
                     GRIIGKQGRVAKAIRTIVYGVRINAPKKVRLNIIDDKEK"
     gene            10279..11070
                     /locus_tag="IXT20_002134"
     CDS             10279..11070
                     /locus_tag="IXT20_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4860570.1"
                     /translation="MRKKMLFIPFALLLSACSSVTQESDNTHLSSSISETVTSPEKSS
                     TKTASTTEQTMATSNNEKKTALDQLKEQQPNVPMPLDVPVSSGYLNIAATHTKQGYSI
                     LYYRTDRPLGLNADELNQETPIATYLYQYGFASSQETIQVLQPFEIDTNGQQVDLGSR
                     ITGYQQGAAGSSFLEWQEGNWRIRIRGNNIEGQDPLLLAKEIVAYLEENSLPAPEQFG
                     KITVDMGDTTNRAVEVSWQEPKNAYTITHQDPMSALKMAVSMKRL"
     gene            11240..11761
                     /gene="rimM"
                     /locus_tag="IXT20_002135"
     CDS             11240..11761
                     /gene="rimM"
                     /locus_tag="IXT20_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HAQ4860571.1"
                     /translation="MTEYLNVGKIVNTQGIKGEVRVISTTDFPEERYKKGTVLTLFQD
                     GKAPVELTVKSHRKHKNFDLLSFEGHPSINDVEKYRDGILKVSKDNSVDLAENEFYYH
                     EIIGLHVLEDGTELGKVKEILSPGANDVWVVQRPKKADILLPYIASVVKEVDLEAGVV
                     RVEIPEGLIDDED"
     gene            11751..12512
                     /gene="trmD"
                     /locus_tag="IXT20_002136"
     CDS             11751..12512
                     /gene="trmD"
                     /locus_tag="IXT20_002136"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HAQ4860572.1"
                     /translation="MKIDVLTLFPRMFEGPMGESIIGKAVDKGLLDINISNFRDYSDN
                     KHQTVDDYPYGGGAGMLLKVQPIYDNIQAIEKAAPDVKKRVILLDPAGKRFDQKMAEE
                     FSQEEHLVFICGHYEGYDERIRTLVTDEVSLGDYVLTGGELGAMVMIDATVRLLPDVL
                     GNQTSAQTDSYSTGLLEHPQYTRPAEYKGMKVPEVLTNGNHKLIEEWQLKESLRRTYQ
                     RRPDLLETLEWTPQMTKFLEEIKKEEQEKTADEAK"
     gene            12648..12995
                     /gene="rplS"
                     /locus_tag="IXT20_002137"
     CDS             12648..12995
                     /gene="rplS"
                     /locus_tag="IXT20_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HAQ4860573.1"
                     /translation="MNPLIEELTKEQLRSDIPAFRPGDTVRVHAKVVEGTRERIQLFE
                     GVVIKRRGAGISETYTVRKVSNGVGVERTFPLHTPRVAKIEVVRYGKVRRAKLYYLRA
                     LHGKAARIKEIRR"
     gene            13253..13546
                     /locus_tag="IXT20_002138"
     CDS             13253..13546
                     /locus_tag="IXT20_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2087 domain-containing protein"
                     /protein_id="HAQ4860574.1"
                     /translation="MVYTYTEQEKMKVLDTFMNDGKVKQVPSSTKKKYILLKEVLKRF
                     DHGVTYTETEVNSILLNVFSSGDYVEQRRYLITFGFFKRSSDGRAYQMMGIEN"
BASE COUNT         4831 a         2576 c         2913 g         4057 t
ORIGIN      
        1 tttttttttg ctatatgaca ggaatgaaag gtgtttgcaa gcttaatgtg aggaacactt
       61 tccatatctg ttctatactg gataggagaa gtttggaaat atccagaaaa cgaaatcaat
      121 cgaggtagcc acatgaagaa taaatggata aatcgaagta aaaaaaagaa agtaacacaa
      181 agatcatcag ataaagtcat ccgtcttttt ccatcgtata aagaaggatt aacggcagat
      241 caagtcaagg aacgaataga aaaaggtgca gcaaataatt cagtcgatcc cacattcaaa
      301 acaaatcagc aaatcgtatt ggaaaacata tttacgtatt tcaatttgat ctttcttatc
      361 ttagccattt tgctttgttt agtagaatcc tataagaact tgactttttt accagttatc
      421 atagccaata caggaatcgc catttttcag gaaattcgat ccaaaaagat cttggacgaa
      481 ctaaatgttt taaatacagc aaaagtcaat gtcatacgag acgggataga acaaacaata
      541 gatatagaag accttgtaat agatgacatt gttattttag agactggata tcaaattcca
      601 gcagatgcag aggtcgtgga agggaaactg caagttaatg aggctttatt gacaggcgaa
      661 gcagatgaaa taaccaaaga aataggtgat ccattaatgt ccggcagttt cattgtttcc
      721 ggaaaagctt acgcccgatt agacaaagtt ggagcagatt cgtatatttc ccagctgaca
      781 gtcaaagcca agacaatggg agaaggagaa cagtcggaga tgattgcttc tcttaatcaa
      841 ttgatcaaat ggattgggat catcattatt cctatcggta tcctgctttt ttctcaaagt
      901 tactttttta atggaaacac gatcaaagaa agtgtcgttg caatggaagc agcattgatc
      961 ggtatgatac ctgaaggact gtacttgctg acgacgattg cattagcatt aagtgcgact
     1021 aaactagcaa agcaaagagt tcttttacac aatatgaaaa gcattgagac attggcaagg
     1081 gtaaatgtcc tttgtgtaga taaaacagga acgatcacag aaaataaaat gtctgttcaa
     1141 aaaatgatcg tctctaaaaa acaagaaata atcagtcagt cagatcaatt ggaagcatgc
     1201 attgctgact atgcaaaagc gatggcagga gacaatgcaa caatggaagc tgttaaagca
     1261 tattttacaa aaaccactga tcaatcctat caccatgtta ttcctttttc ttctgtccaa
     1321 aaattcagca gtgtagcgtt ggcagacaag gtgtatgttt taggagcgcc cgaaatggtt
     1381 ttgcgcgatc aattgccgga atatgcggaa gagtttgttt ctttcgctga aaaaggctat
     1441 cgcgtgttgg tctttggtgt gtacgatggg attcttgaag aaccagtatt gacagaagca
     1501 gtcacaccat taggctatat tctgattgct aatcctatca gaaaagaagc aagagccaca
     1561 tttgattact tcaaaaaaca gcaagtagcc atcaaagtga tttctggaga taatccgctc
     1621 accgtatcca atgtagctgt ccaagcaggg atttcacatg ctgatcagta cgttgatgca
     1681 tctaaattat cagaagaaga atatgaagca gctgtcgaaa aatatacagt ttttggacgc
     1741 gtcaagccag aacaaaagaa aatcttcgtt caattgataa agcgtaacaa tacagtagct
     1801 atgacaggtg atggcgtgaa tgatattcta gccatgaaag aagcagactg cagtatagcg
     1861 atggcttccg gaaatgaagc cgccatgcag gcctcacaag tggtcttact tgattctgac
     1921 ttttcacgca tgccagaagt tgttgcagaa ggacggcgcg ttgtgaacaa tattgagcga
     1981 tcagccagct tgtttttagt gaagaatatt ttctcctttt tgctctcact gttttcagtg
     2041 atctttgctc tgacctatcc tttagaacca tcacaaatca cattgattag tttgtttact
     2101 atcggacttc catctttctt gcttgctcta gaagaaaaca aaaagcgaat cagagggaaa
     2161 ttcatcatga atgtgatgga aaaagctgtg ccaggtggac taacagatat gatcgtagtc
     2221 ggtgcgttgg tgatctgcgg ggtgactttg aatctaaata aaacagatgt ttctacagca
     2281 tctaccatgc ttttgattgc agtagggttc ctcgttctct ataaaatctg ttcaccgctg
     2341 aataaattca gaagtcagat cattcttttc tgtgctagcg gtaacttttt ttcggtcatc
     2401 tttttgcata agctgttttc aatcaccggt atctcagctg tatcactttt gctgttgagc
     2461 attctatttt tctcggcaga ttcgattttt aggcatttga ccacattggt agaatatttg
     2521 tttgaacgta aaaatgaaaa agagcaaaag ccgtttagtc gcgtagctaa aaaatttcta
     2581 tcgatattta aacgagaaaa ttgacgaaaa gtatctcaaa aaagagtaaa aaagaacaac
     2641 cactcgtgta tctttcgaag caggatgagt catccaactt taaaacgcac gagcagttgc
     2701 tcttttttac attttcggaa tggtaacggt gaaagtcgaa cctgttccaa gatgactgtc
     2761 tacagtaatc gtaccattaa ggagttgcac gtagtctttt acaatcgcta accctaatcc
     2821 ggtcccgcct gaatgtcggc ttcgcgcctt gtctacgcgg taaaaacgct caaagatccg
     2881 ttcctgatcc tcaatgtcga tacctatacc aaagtcttgg acagaaaacg atagatcatc
     2941 tgttgcctga tagcggataa taatctcgct gtcgcttttt gaatattgga cagcattttc
     3001 aatcagattt ttgataattg gataaaggat atctgtttta gtagtaaatg gttcgttctt
     3061 tccaatcaat cgaatcgtca accgtttctt ttcgatgatt ggctgataat tttgaacgat
     3121 ttgctggaaa taaggttctg tataaattgt ctggatctcg taagaattct cgccatcttt
     3181 tgacagctgg atgatttccc gaatcagttt atctaaccgg atcgcatctt tttgcatgat
     3241 ctcaagaaaa gaagtcagag tttgcgggtc atcttttgca ccatccagta aagtctcggt
     3301 aaaaccaatc agtgaagtca caggagtttt taattcgtga gagacgttgc caacgaaatc
     3361 cttttgtagc ttttctaaac gtcgcacttt cgttaagtca tacgccaccc ctagaatctg
     3421 tccggtacct tctgaattag taaagtagcg caaactcaag tctaatgtct gccgaccttc
     3481 tgtaagggtg atctcctgat ggatcagcgg atgctcagga gtcacttgat gaatcagctg
     3541 aatgagtttt ggctcttgaa tgacttctgt gtaccgctgt tcaggttgat agcttaccac
     3601 gtttaaatgt agctgcatct ttggattaag cagtagtaaa cgagaatcag catcatcaat
     3661 caggaatacg ccaatcatca gttcgttcag taacgtatat aattgctctt ctgtggtagt
     3721 acacgcccga tacatcttac tcatttgttc gctcaacgca ttaatcgtct gatacaattc
     3781 ttcccattga tcgggtgttt gcatgattac ttccgcctga ttaggatttt gcaccatttt
     3841 ttttagtacg ggcagcacgg ttttcaatgg ctggtttttt tgatagatca gataatagat
     3901 cattcctgca agaacaagga agaaagacag aaagaagaaa aagacccaac ggcgaaaact
     3961 ctcagttctc ggtaaaaaaa cgcttgtcgg ttctgcgata cgtaaaatgg cttctaactg
     4021 gccacttttt ttgactggta atgcgacata taaaagatcc tcattcaaag tagtactttt
     4081 gcgcaaagcg ctacctaggt tcccaccatt caatacggct ttgatttcgg gacgggagtt
     4141 tcgctgttca ttcaatgttt cattgtttgt gtcaaataag atctttccag tagcatccat
     4201 taaagtaatc cgttcgttcg actgatggac gaattcttcc aaagcagttt tgttttgcgc
     4261 tgcttggagg ttttcgacat ctaattgccg gatcagcagt tgtgcttttt ttgtcaggta
     4321 gctttcttgc tgttccacga tctgctgttg aaaataatgg gaaatcaatt gccagccacc
     4381 aaaaaacaaa ctaactaaaa gaataagaaa gaagagacgt tcataaattt ttttccattt
     4441 catcgtttgg gttcctgaaa cttatagcca aatcctctaa tagtcaccaa gtactttggg
     4501 tgttttggat ctcgctcgat tttttctcgc aaatgactaa cgtgtacatc tacgattcgg
     4561 ctttgtcctg cgaagtcaaa attccaaatg cggtctagca atgtgtctcg gtcgatcaca
     4621 cggtcttttc gtttcatgaa ataaacaagt aattcaaatt ctttcggtgt tagttcaatc
     4681 agatggtcgt ctacggtcac ttgataattg gtcaaatcag cagtgatttg tccaatcgat
     4741 agatattcag gctcagcttc atcttttgat tgcttacgag gttcaattcg tctgaatatt
     4801 gctttcatac gggctaatac ttcgcgagga ctaaatggtt tggtcaaata gtcgtcagca
     4861 cctatttcta ggccaatgat ccgatccact tggtcatctt tagccgtcag catcaaaatc
     4921 ggtgtttcat ttttttcttg acgcagccgc tgagtgatct ccatgccatc catcgttgga
     4981 agcatcacat ctaaaatgat gaaatcaaat gattgttcta atgctaattc taatccttcg
     5041 cctccatctg tggcactggt taccttgtag ccttcttttt ccaaattgaa cgtcaagagc
     5101 gttacaatag aaggttcatc atcaacaact agtatttttt tcattcaaat ctcctttaaa
     5161 taggtttcat tctgtttata ttttagcata gaatcagtac aaagggctat tttacaaaag
     5221 aaataaatgg tataatagga aggcactttg ctgaatagaa aggaagagca gtcaatgatt
     5281 ggaatcagtg catgtttagg cggtctggcc tgtcgctatg atggacagaa aaaagaagta
     5341 ccagaattga tggaactagt tgaaactgga gaagctgtca tggtctgtcc agaagtgatc
     5401 ggcggattac caattcctag agatcccgct gaaatcattg gtggaaatgg attcgatgtg
     5461 tggaaagatc aagcaaaagt atggacgaca agcggcgaag atgtaacgga agcgtacaaa
     5521 aatggcgcga tcaaagccta tcaaaaattg caggaaaaac acattgatct agtcatcatg
     5581 aaggaaaaaa gcccttcatg cggaacccat tcgatttatg acggaagttt ttccggaata
     5641 aaaaaaaatg gtgccggagt ggctactgct tattttattc agcaaaaaat gactgtgctc
     5701 tctgatgaag aatggctgaa aactagaggt gagcagaatg gaaatcgaaa agaccaatcg
     5761 aatgaacgcg ctctttgaat tttattcgac cttattgaca gaaaaacaaa tgaactacat
     5821 ggaattatat tatgcagatg atttttcatt aggtgagatc gcggaagaat acgaagtaag
     5881 ccgacaagca gtatatgaca atatcaagcg gacaagcaag atcttggagg attatgaaaa
     5941 aaaactccat cttttttcta attatattgt tcgagaacag gtattggaaa atatgactgc
     6001 atatattaca gataagtacc cggaagataa aaaactactt gaatatgtac agcaaattca
     6061 agcaatcgaa gaataaataa aaggaatgaa gaaaatatgg catttgaaag cttaactgaa
     6121 cgtctccagc aggcgatggg aaaaataaga aaaaaaggaa aagtatcaga agctgacgta
     6181 aaagaaatga tgcgggaaat ccgccttgcc ttactagaag ccgatgttaa cttgcaagta
     6241 gtcaaagatt ttactaagcg cgtacgcgaa cgtgcggtag gggcagaagt gctggaaagt
     6301 ttatcacctg cacaacaaat cgtaaaaatc gttgatgaag aattgacgat cacacttggt
     6361 tctgaaacag cagagttaaa taaatcacca aaaataccaa ctgttatcat gatggctggt
     6421 cttcaagggg caggtaaaac gacctttact ggtaaattag ccaattattt aaagaaaaat
     6481 gaaaatgctc gtccgctcct aatcgcaggg gacgtatatc gaccagctgc gatcgaccaa
     6541 ttgaaagtat taggtcaaca gctagacgtt cctgtatttg atatgggaac tgaagtcagc
     6601 ccggtagaga tcgtccgtca agggatggag ttagcaaaag aaaagaaaaa cgactatgta
     6661 ttgatcgata cggcaggacg tctgcatatc gatgaaacat tgatggatga gttgaaacaa
     6721 atcaaagaat tgacgcaacc aaatgagatt ttgcttgttg tcgatgccat gactggtcaa
     6781 gatgccgtca acgtagctga tagcttcaac cagcaattag gtattacagg ggtcgtcatc
     6841 actaaacttg atggggatac tcgaggcggg gcagcactat cgatccgctc agtgacaggc
     6901 gcaccaatca aattcatcgg ttctggtgaa aaattgactg acttagaagt attccaccca
     6961 gatcgtatgg ctagccgtat ccttggtatg ggggatatgc tgacattgat tgaaaaagcg
     7021 cagcaagact acgacgagaa aaaagcggaa gaacttgcac agaaaatgcg ggaaaattca
     7081 ttcgatttca atgactttat tgaacagctt gatcaagtga tgggtatggg cccactggaa
     7141 gatctaatca aaatgatccc aggaatgaat caagtccctg gaatcgaaaa cgtcaaggta
     7201 gatccaaaag atgttgaacg caaaaaagcg atggtttatt ctatgacgcc agcagaaaga
     7261 gcaaatcctg atctgttgaa cccaagccgt cgtcgccgaa ttgctgccgg ttctggaaac
     7321 agtgttgttg aagtgaatcg aatgatcaaa caattcaagg aatcaagaaa aatgatgcag
     7381 caaatgtcca aaggggatat gaacatccca ggtatggacc aaatgtttgg ttcaggcgtc
     7441 aaaggaaaac taggcaaaat ggcgatgaat cgaatgatca agaaaaataa gaagaaaaag
     7501 aaaaaacgga aataagatga aaaaaacagc ttcggtacag actgaaaatg tctactgccg
     7561 gagcttttct atcagcaatt tttattcttt actaatacaa ttactctaaa cgaagaggtg
     7621 gcacagatga gatgtccgtg gagtgaacaa acagaaagta tgcggcaata tcatgatact
     7681 gtctggggtg ttcctgaata tgatgaccaa aagctgttcc gaaaattgat gcttgatatt
     7741 aatcaagccg gattgagctg gcagacgatt ttaaataaaa gtgaagcatt cgatgaagca
     7801 tatgatcgat tcgatatcaa aaaagtcgca aattatggag aaacaaaaat cagtgaacta
     7861 atggaaaaca aaggaattat tcgtaaccga agaaaaatcg aagcagccgt ccaaaacgcc
     7921 aaagcagtct tgaaaattca agaagaaatc ggcagttttt cagagtatct ttggtcgttt
     7981 tctaatggaa aaatcgttga ttcttcttat cattcacaag aagaagtccc aacaacaagt
     8041 cctctttcag atgaattagc aaaagatcta aagaaaagag gatttaaatt tatcggaagc
     8101 acaacaatct atgctttttt agaagctgtc gggatcatca atgatcacct tgacagttgt
     8161 ttccgcaaag aagaggtcgt acgtatcggc agaaagggcg aataaagcat gcgaaaaatc
     8221 atattttatg gtgctataag tttagatggc tatttggcaa cgaaaacgga tgatcttcaa
     8281 tggctttttg acactccaac aggtaagcaa actacttatg aagcttttta tgaaacaatc
     8341 gatacgacca tcatgggcag aaaaacttat caggaagcaa aaaaatattt ggagaccgaa
     8401 gcaatttatc cagagaaaaa gaatttcgtt ttttccaccc acgcacaatt acaattgcca
     8461 gatgctgagg tcgtaaacga agatcctgtt gcttttctaa aaaatctaca aatgacagaa
     8521 ggaaaagata tctgggttgt gggtggcggt cgattgatca aaccgctatt ggaacatcaa
     8581 atgattgatg aatggtatat ccagattacc cctgtattat taggggacgg gattccttta
     8641 ttccaaaaag gaacatatga agcgcgtttt gaaatgctag acacgacacg attcggtgaa
     8701 tttatcgaat tgcattatgt acgaaagtag aagtaaataa aataaaaaag gcaactatat
     8761 gcagaaatgt cctgtacata gttgcctttt ttattaccgt tatgcttttt actctgttaa
     8821 tttgttagaa aaatctagat acgcatctaa aatcgtttgg aagaaactta atgttccttc
     8881 aacgatatta ccattttcat caattagatt cgcaacgttt ccgatatatg cttccggttg
     8941 ctgcaaggtt ggcacattca aaaagacaag tgattggcgt agatggtggt ttgcgccaaa
     9001 gccgccgatc ccacctggtg atgcagtaac gactaaacct ggtttatgat cccaaacact
     9061 gtgtccataa ggtcttgagc ctacatctaa agcatttttc aatactgctg gcacagaacg
     9121 gttgtattca ggagtaacga aaacgacacc gtctagtccc ctcacttcct cacgaaaacg
     9181 agtccattca gcaggagcct gttctggagt ttcgagatcc tcattgtaaa atggcagatc
     9241 atcgattttt acaaatacac tttcgtaccc ttcaggaagc aatttcgcaa aagcttctgc
     9301 tacttttttg ttgtaagaat cttttcttaa actaccaata aaaaatccaa ttttttttgt
     9361 catacgattt ttcctccttc aaactatcta taataatctt acaaaagata agtaactgga
     9421 gcaaataaaa acacttactg acaaaagcta catagaagat agattactta tttttaacaa
     9481 aaaagaagtc taaaatcttc tattttctaa aaaaaatcga atttaatcaa gctgtaaaga
     9541 aaaaaagctt tacagggtaa aaaaaaactg ttacactaca tcttgtgaat aaaacaatgg
     9601 aggtggaaat aaataatggc agttaaaatc cgtttaaaac gtatgggttc taaaaagagt
     9661 ccgttttacc gtatcgtcgt agctgattca cgttctcctc gtgatggacg tttcatcgaa
     9721 actgtaggta cttacaatcc tttgaaagac cctgcagaag tagttttaaa agaagactta
     9781 gttcttgact ggttatcaaa aggtgcacaa ccttctgaca ctgtacgtaa catcctttca
     9841 aaagaaggcg ttatgcaaaa acaccacgaa gctaaattct caaagaaata aggtgaccgg
     9901 atatgaaaga tttaagcgac ttagtcttaa caatcgttcg tccgctagtc acttatcctg
     9961 atcaagtcaa gttagacgtc gtagagtcga aagactttta cgagtacaat ctaacagtgg
    10021 ctcctgaaga tattggccgt atcattggaa aacaagggcg tgttgctaaa gcaatccgca
    10081 caatcgttta cggtgttcgg atcaatgcac cgaaaaaagt gcgtttgaat atcatcgatg
    10141 ataaggaaaa gtaaataaac gtgctgtact cgaattttcg agtgcagctt ttttgttttt
    10201 tttattaatt gaagaaatga ttatttggca aaacaagcaa gaaagcgtat ccttatagga
    10261 ggagggagtg aacagccaat gagaaagaaa atgttattta ttccatttgc tctattgtta
    10321 agcgcttgta gttcggttac tcaagaatca gacaatacac atctatcaag cagtatatct
    10381 gaaactgtta ccagtccaga aaaaagtagt accaaaacgg cttccaccac tgaacaaaca
    10441 atggctacct caaataatga gaaaaagact gctttagacc agttgaaaga acaacagcca
    10501 aatgttccca tgcctctaga tgtaccagtc tcttcaggct atctcaatat cgcagcgaca
    10561 catacaaagc aaggttattc tattttatat tacagaacag atcgtcccct cgggttgaat
    10621 gctgacgaat tgaatcaaga aacgcctatt gcaacctatc tatatcaata tgggtttgct
    10681 tctagtcaag aaacaatcca agttttgcag ccatttgaga tcgacacaaa tggtcagcaa
    10741 gtggacttag gttctcgaat cacagggtat cagcaaggtg cagcaggttc aagttttctt
    10801 gaatggcaag aaggaaactg gcgtattcga atccgtggga ataatataga aggccaagat
    10861 cccttactat tagctaaaga aatcgtagct tatttggaag aaaacagtct accagcacct
    10921 gaacaatttg gtaaaattac tgttgatatg ggtgatacga cgaatcgagc cgtagaagta
    10981 agctggcaag aaccgaaaaa tgcctatacg atcactcatc aagatcctat gtctgcgttg
    11041 aagatggcag tttctatgaa gaggttgtga aatcagcgcg ctgatctgag cccatcattt
    11101 aactccaagt cacaggaagt aatcaaaaat agcacgccgc atttttgcat aattttggct
    11161 taatacctta aaagaaaact agaattgcct cttcacacat gctaaaataa aacagacaaa
    11221 aaagaaatgg ggatataagt tgactgaata cttgaatgta ggaaaaatcg taaacacaca
    11281 aggtatcaaa ggcgaagtcc gtgttatctc aacaacggat ttcccagaag aacgttacaa
    11341 aaaaggaacg gtgctaacac tattccaaga cggaaaagca cctgtggaat taacagtcaa
    11401 aagtcatcgc aaacataaaa attttgactt attgagtttt gaaggacatc cttctatcaa
    11461 tgatgtggaa aaatatcggg atggcatatt aaaagtgtca aaagacaact ctgtagatct
    11521 agctgaaaat gaattctatt accatgagat catcggtctg catgtattag aagatggaac
    11581 tgagttaggt aaagtaaaag aaatcttgtc accaggtgcc aatgatgttt gggtcgttca
    11641 acgtccgaaa aaagctgata tattgcttcc atatatcgct tctgtcgtca aagaagtcga
    11701 tttagaagct ggagtggtcc gtgtagaaat tccagaagga ctgattgacg atgaagattg
    11761 atgtattgac tttatttcca agaatgtttg aaggtcctat gggggaatcc atcatcggta
    11821 aggctgtaga taaaggatta ttagatatca atatctctaa cttccgcgat tattcagata
    11881 acaaacatca gacagtagat gattatccat acggcggtgg agcaggtatg ctgctcaaag
    11941 tccagcccat ctatgataac atccaagcca ttgaaaaagc tgcaccggat gtcaaaaaaa
    12001 gagtaattct tcttgaccca gctggtaaac gattcgatca gaaaatggca gaagaatttt
    12061 cacaagaaga acacttagta ttcatttgtg gtcattatga gggatatgat gaacgtatcc
    12121 gcacattagt gacagatgaa gtttccttag gagattatgt ccttactggg ggagaattag
    12181 gtgcgatggt catgatcgat gcaacagttc ggcttttgcc agatgtgtta gggaatcaga
    12241 cttcagctca aacggattct tattcaaccg gtttattgga acatccccaa tatactcgcc
    12301 cggcagaata taaagggatg aaagtaccag aagtattgac aaatggaaat cataaattga
    12361 ttgaagaatg gcaattaaaa gaatcactaa gaaggaccta tcaacgtcgg cctgatcttt
    12421 tggaaacact tgaatggaca cctcaaatga cgaaattttt agaagaaatc aagaaagaag
    12481 aacaagaaaa aacagcagat gaagccaagt gactttttct aaaatcttct ttacagtagg
    12541 ttgataatat gttagtattt tatggtgagt gcaaaagcac ttaactatta cgatattccg
    12601 ctgtgagaaa agctcataag aatatttgga ataaggagaa gaaaaccatg aatccattaa
    12661 tcgaagaatt aacaaaagaa caattacgtt ctgacatccc agcgttccgc cctggtgaca
    12721 ctgtgcgtgt tcacgcaaaa gttgttgaag gtactcgtga acgtatccag ttatttgaag
    12781 gtgttgttat caaacgccgc ggtgctggaa tcagcgaaac ttatacagta cgtaaagttt
    12841 ctaacggagt gggcgttgaa cgtacattcc cattgcatac accacgtgtt gcaaaaatcg
    12901 aagtagttcg ctacggtaaa gtacgtcgtg caaaattgta ctacctacgt gcattacacg
    12961 gaaaagctgc tcgtatcaaa gaaatccgtc gttaatcatt gttatcagac tgagaaagaa
    13021 cccttggtat tcaagggttc ttttttctta gcatgtaatt tgaacaccct gatccagttt
    13081 tttctttaat tattggtagg ggagaataat ggattagaat aaatctacct ataagtagta
    13141 gtaaaaaaaa gagcactatg ataatatctt tcttaagagt aaccgcttta ataaaaaatc
    13201 cagaatattc taacataaat ggagtattct tagaatcaaa aggagaaaga agatggttta
    13261 tacttatacg gagcaagaaa agatgaaagt tttagacaca ttcatgaatg atggaaaagt
    13321 aaaacaagta ccaagttcaa caaaaaagaa atacatccta ttgaaagaag tcttgaaacg
    13381 ctttgaccat ggagtgactt atacagaaac agaggttaat tcgatactgc tgaacgtatt
    13441 ttctagtggc gattatgtcg aacaaagacg ttatctaatc acatttggct ttttcaaaag
    13501 aagttcagac ggtcgggcat atcagatgat gggaattgaa aattagaagt tttaatttat
    13561 actcattgaa aaaagttcaa gtatgcgata ttggttctac accgcatact tgaacttttt
    13621 ttgtcatctg tatctataat acaaagccaa gaacgttatt caaaatatgg atagctatga
    13681 taaaagctaa tgagcgattc gcagttaaat aagcaatcgt cagtaaaatc gttccaggag
    13741 cgtacatgaa aaaatcaatt ggatgcgtta catgaataaa gataattctc ttttatttgt
    13801 acgaataaat accaaaacgt acctcctaaa attagatttt ggacagatgc cctttaaaaa
    13861 gataacaaga tttcgttctt gaaacaatat atatgctttt attccaatga ggttgtgaaa
    13921 acagtatttt gtcctgagcg ttagaaaatt gaaaatgggg aatagcaact cgtactttac
    13981 cattttttaa gtgaatacaa gatcagctaa ttgaacaccg tttattcgtg atgagggaca
    14041 caacactgca gtcgacacat ttctctttta tgttcaaaga taacaaatat ttaaaacaaa
    14101 gaaaaatagt agataatccc gtcctaactc ttttattctt ccctcatagc ttccaaattt
    14161 aagcaatact tcaattaatc aagttttatc tttttttttc aataatcgta aaaaaagagt
    14221 attttttatt taaaaataaa atctttgtat taaaggacaa gactaaaata accttagaat
    14281 aaaaagagaa aaaaagcccc aaactttcta tacatcaata aaataataaa aattaaattt
    14341 agaataacgt gtaaaggatt atttgatgat tttttat
//
LOCUS       DACGLE010000066        13975 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0534
            SAMEA3304449-rid9560453.denovo.070, whole genome shotgun sequence.
ACCESSION   DACGLE010000066 DACGLE010000000
VERSION     DACGLE010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304449
            Sequence Read Archive: ERR985064
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13975)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13975)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702225.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702225.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 20:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,045
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 27 of 67
            Pseudo Genes (incomplete)         :: 25 of 67
            Pseudo Genes (internal stop)      :: 19 of 67
            Pseudo Genes (multiple problems)  :: 4 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13975
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304449-rid9560453.denovo.070"
                     /strain="VREN0534"
                     /db_xref="taxon:1352"
     gene            140..460
                     /locus_tag="IXT20_002139"
     CDS             140..460
                     /locus_tag="IXT20_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4860575.1"
                     /translation="MKKRLLWCLLFLSLSLTSQPVFAEETQRSPITGTRLYKNVYVQT
                     TDLDSAIPETEERSLQPRFGYFWYCPTCDYESCWHFLHSTAAAFAFTHTQKYDGHHPV
                     IYEV"
     gene            912..1112
                     /locus_tag="IXT20_002140"
     CDS             912..1112
                     /locus_tag="IXT20_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAQ4860576.1"
                     /translation="METGTVKWFNSEKGFGFITAENGNDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEEGQRGPQATNVTKA"
     gene            1259..4048
                     /locus_tag="IXT20_002141"
     CDS             1259..4048
                     /locus_tag="IXT20_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HAQ4860577.1"
                     /translation="MKPRTYAVVDIETTGTNPKEDRIIQFGCVMIESGRITSRFSIDI
                     HPGRKISKQIQHLTGITNQRVQKAPYFEDVAQTIYNLLADTIFVAHNIYFDYNFLNHE
                     LMRCGLPSLKIPGIDTVELAQIFLPTEPSFRLADLSESLGFIHENPHQADSDAEVTGQ
                     LLLLIEERMRKLPIITLEQIARLSRHTGMDTSRFIYHVIEEMKEKPEPLDPSLEIVDG
                     LALQKKEVELFTSVHYGERTYPRKKQAKEKMFGKTLMYRKEQNRLMNAVYDHFTKDES
                     KDLMIEAATGMGKTIGYLLPLSYLATPEKPAVISTVSLVLQQQIIEKDIPLLNQLLDQ
                     PIQPVIIKSYRHYIDLQRFKGTLVEPPEQKQYALYQMAVLVWLTQTKTGDLDELHLTN
                     LNHSFFADIAHRGIGFLARNQSFYEQDFVRFLQKKIRQSNFLIVNHAFLIQETQRKEP
                     LLPASPFLLIDEVHHLPEIAEKVNDKRLSINYFHKQIQQLEEPNQLFDRIQSYLPKEH
                     EALRWLSIYREELSALCEAQICMAEGLMSLVSGKQPNGQYPEEVMMTKEMLDQLPLES
                     GTAIDHLQLFYQEIQQLQSQMRLTFEIIQESWTNQMRIDLASLYSLFDQLDKQYLLMD
                     QWLEDWRDHLIHWFVPGNTAQQMIFHLHDFQAAMLPSTVWYPRYDRILYIGGTLKIGP
                     NRHYLSKRLGIEAAPLKVIASPYDYEKQARIYVPKEAPAVHQVSNQAYIDYLETALTG
                     LIEQEDRPILVLFTSHDVLQKVYQKMHLSMLEKGREILAQGFGGSREKLLKRFILSEK
                     SVLFGADSFWEGVDLPGDALQIVVVTRLPFDNPLRPMVKARNAYLESEGINPFYQESV
                     PKAALKLRQALGRLIRTETDRGVLLILDRRLVTAKYGARIIHALPKKVPIKELAMEEI
                     YQDIHEFLKKNEEGS"
     gene            4095..4622
                     /locus_tag="IXT20_002142"
     CDS             4095..4622
                     /locus_tag="IXT20_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HAQ4860578.1"
                     /translation="MKIKERRPLKKKQTKEKTINRVLLGLIVFLLAVIVISSIFYLRS
                     SRPAVQAKKEAVEIAKKYANLEEADHFYWFTRKKTYFSLTGTNDKGKEIAVIIPKSGE
                     KVKVLDQTKGLTENEAKQTIAAAHPEIQIEKAALGMYDDQPVWEVVGKETNGKLNYYL
                     IGFESGDEVKTIKGI"
     gene            4643..5833
                     /locus_tag="IXT20_002143"
     CDS             4643..5833
                     /locus_tag="IXT20_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAQ4860579.1"
                     /translation="MELSKRTKRLEPSVTLAAAAKAKALKAKGRDVLSLTVGEPDFAT
                     PENIQEAAIEAIRNGKASYYTPTAGIPELRQAIVDYLKKNYGLVYEPSQTVVTDGAKF
                     ALYTLFQTILDPQDEVIIPVPYWVSYGEQVKLAEGLPVFVKGEESNSWKVTVEQLEQA
                     RSEKTKAVIINSPSNPTGMIYSKNELQAIGEWAVKNDILIVADDIYGRLVYNGNEFTP
                     IATISEAIKNQTIIINGVSKTYAMTGWRIGYAVGNSEIINGMIAVASQSTSNPTAVSQ
                     YAAVEALKGEQDTVEEMRNAFEERLNTLYPLVAELPGVSLEKPQGAFYLFPNVKETLR
                     MCKYENVTQWVEDLLEETGVALVTGEGFGAPENVRMSYATDRATLEEAVRRIKQFIES
                     KSQN"
     gene            5873..7171
                     /gene="asnS"
                     /locus_tag="IXT20_002144"
     CDS             5873..7171
                     /gene="asnS"
                     /locus_tag="IXT20_002144"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HAQ4860580.1"
                     /translation="MEQIQIIDSKNHVGETVTIGAWVANKRSSGKITFLQLRDGTAFF
                     QGVVVKSEVSEEVFQTAKNLHQETSVLVTGEIREDSRSKFGYEIGITNVEVVSESHDY
                     PITPKEHGTDFLMDHRHLWLRSSRQHAIMQIRNEIIRATYEFFNQHHFVKIDPPILTG
                     SAPEGTTDLFETNYFDQKAYLSQSGQLYMEAAAMAFGKVFSFGPTFRAEKSKTRRHLI
                     EFWMIEPEMAFTHQEESLEVQEQYVAFLVQNVLDHCEYALDVLGRDKEVLKTYTKLPF
                     PRITYDDAIELLKKNGFDDIEWGEDFGSPHETFIANSFEQPVFILNYPKAIKPFYMKP
                     HPTREDVVICADMIAPEGYGEIIGGSERATDYDFLLEQIRNHGLDEKEYSWYLDLRKY
                     GSVPHSGFGLGLERTVTWIAGIDHVREAIPFPRLLNRIYP"
     gene            7966..8640
                     /locus_tag="IXT20_002145"
     CDS             7966..8640
                     /locus_tag="IXT20_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HAQ4860581.1"
                     /translation="MKKRILFIGDSITDAGRYRAEATDLGKGYPLLIAGKLISQYPTM
                     EFECMNRGIGGDKIADLQVRWQEDCLALQPDYVSILIGINDTWHNTSDQAIFGTEKSA
                     QQFESVYRKLLNELQTAEICNIILMEPFVLPITDDRHGWRKDLDPKIQIVRKLAKEYG
                     ALLVPLDGIFNALGIAYGFSKYTREDGVHPTIGGHEIIAQSWIDAIVAVDFFSGDKNE
                     RKKSEK"
     gene            8637..8978
                     /locus_tag="IXT20_002146"
     CDS             8637..8978
                     /locus_tag="IXT20_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4860582.1"
                     /translation="MMEIRWAVKEDLPQINQLITDHPESFVKAKTAADLNDKNKLLLV
                     AVTKEKRIIGFAQADVQQSEERLDQRPSGKLIQHTLVTKENTEEIQKKFAEYLMLEAK
                     KRRIKSIFAQF"
     gene            9008..9367
                     /locus_tag="IXT20_002147"
     CDS             9008..9367
                     /locus_tag="IXT20_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HAQ4860583.1"
                     /translation="MSCLGNVIWFIFGGLIGGLSWLAAGILWCITIIGIPIGMQCFKI
                     ASLSFAPFGKEVIYHQSGMSLIVNIIWLIVSGLPIALAHVVSGILLCITIIGIPFAKQ
                     SFKLARLALMPFGAEIV"
     gene            9561..9749
                     /locus_tag="IXT20_002148"
                     /pseudo
     CDS             9561..9749
                     /locus_tag="IXT20_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329861.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
     gene            9891..11975
                     /gene="cadA"
                     /locus_tag="IXT20_002149"
     CDS             9891..11975
                     /gene="cadA"
                     /locus_tag="IXT20_002149"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HAQ4860584.1"
                     /translation="MKSYRLEGLDCANCAMKIEKGVQKINGVKEATVNFTSGKLTIDA
                     EEDHLATIEQETKKVVKELEPDVKVTEIDKEKVSEHGNEKKRNTLFRILFSLAGIALL
                     LLFDFNEPIRLIGYLLIYLLIGYDVVKKAVMNIVKGKIFDENFLMSVATIGAMLIGEY
                     PEAVAVMLFYQIGEYFQGLAVSHSRKSIRELMAIRPETAHVQTAEGLMTVNPEDVLIG
                     QFVLVKPGERVPLDGTIIEGESLVDTSALTGESVPKSVYVGETVLSGFINKNKPFLVQ
                     VEKSYENSTISKLLELVENASSKKAPAENFITKFARYYTPVVVGLAVLLAVLPPLVVS
                     RAAFSEWIYRALTFLVISCPCALVISVPLSFFGGIGGASKIGVLVKGSNYLELLAQTE
                     TVVFDKTGTLTKGDFSIQTIDPTGIDPKKFMQYVASAEQFSTHPIAQSVLEGYAGPLL
                     PTANIQEFAGEGILAEVDGKQVLVGNHKLMERFEISFPSSQEIGTLLYLAIDQSYSGY
                     LVIADTLKEDAVDALVQLKQAGVKNTVMLTGDSKKIADHIGKQVGVDKIYSELLPEDK
                     VQRLEEILQSNNKKTAFVGDGINDAPVLARADVGIAMGGLGSDAAIEAADVVIMNDQP
                     SKIAEAIHLAKKTLKIVKQNIVFAIGIKILVLSLGAFGFASMGDAVFADVGVTVLAVL
                     NAMRSLHVKNKR"
     gene            12286..13752
                     /locus_tag="IXT20_002150"
     CDS             12286..13752
                     /locus_tag="IXT20_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ4860585.1"
                     /translation="MNNKQLRWFTVALIAFNMVWGMGNVVNNYAQQGVSVVTSWLLIL
                     AIYFIPYALIVGQLGSAFKDSKGGVSSWVENTTSNKRLAYYAAWTYWVVHIPYLAQKP
                     QAILIAGGWAVQGNGNIVNTMSVQMVAVISLIIFLAFLYLSTKGLSTLKVIGGLAGTA
                     MFVMSILFILLAVTAPFINPTMEFATPHMNQVKTYIPTFDFSYFTTVSMLVFAVGGAE
                     KISPYVNSTRNPAKEFPKGMIFLAAMVGICAVLGSVAMGMLFASDNIPKDLMANGAYS
                     AFQILGNYYGIGNSLMIIYALTNMIGQISALAFSIDAPLKILLADADPEFVPNWLRKR
                     NRKGTLVNGYLLTGILVGIIILLPIFGIKDMNELVKWLTNLNSVVMPMRYLWVFLAYM
                     MLNKAYKKFNSEYKFVKNPKIGFIFGAWCFAFTAFACILGMVPKVDYTADPKAWWFQL
                     FTNIITPIVLILLGMILPAIARRDKNKEITEPETVVQP"
BASE COUNT         4635 a         2430 c         2974 g         3936 t
ORIGIN      
        1 aaaaagaggt tgtgacgtgg tcacgacctc tttttgacga ttatgataca aaaaacatca
       61 tttatgaaca aaatgtgaat aacgggcgtt cttgagatat gattttaatt gggaaaaact
      121 tatcgataag gaggtcttta tgaaaaaaag actgttatgg tgtttgctgt ttctatccct
      181 aagtttaaca agtcagccag tttttgctga agaaacacag cggtctccta ttacaggcac
      241 acgtctgtac aaaaatgttt atgtacagac aacagatctt gattctgcga ttcctgaaac
      301 agaagaacgg tctttgcagc caagatttgg ttacttttgg tactgtccga cctgtgatta
      361 tgaaagttgc tggcatttct tacattctac agctgcagct tttgctttta ctcatactca
      421 gaaatatgat ggacatcatc ctgttattta tgaggtatag atatgagcaa aaaagaaaaa
      481 ctaatctcgt tgatcgtatt ggtttgcctt gttcttctgg taactgtcat aggctatcag
      541 aaataccatg agaacatcag acctactaaa gaatcttccc cagcctcgac cgttgaaaca
      601 gaaccgataa gtagtcagaa agaataattt ttttagttta aagaagaaca tcagagcaaa
      661 aaaacaccaa ctgaacaata tgtcgtttat tttaaaaaaa gttcggtttt tcactttatg
      721 taatcgctga gaaaactttt tttaataaaa aaagtcgaaa aactctagct tttttgtaaa
      781 cggtatgata tactatagga gtagtttttg ttcggtaatc tgtggtagct tgtttcaaat
      841 aaacatcttc cagatataca ccattcaagt aattgcaaaa tatgtgttga tacacaagga
      901 ggatttttat tatggaaaca ggtacagtaa aatggtttaa ctcagaaaaa ggatttggat
      961 tcatcactgc agaaaacgga aacgatgtat tcgttcattt ctcagctatc caaggcgacg
     1021 gattcaaaac tttagaagaa ggccaagcag taactttcga tgttgaagaa ggccaacgcg
     1081 gacctcaagc aacaaacgtt actaaagctt aattaaccta tactaaaaaa taaacgccca
     1141 gacatttgcg tgtctgggcg tttttgtcta ttttctttaa aaagcgatga gagtattttt
     1201 ctgcactttg tagtataatt tcaaaagaac attagaggca ggagaagagg aagatatttt
     1261 gaaaccaaga acttatgcag tcgttgatat cgaaacgact gggacgaatc caaaagaaga
     1321 tcggatcatc caatttggct gcgtcatgat tgaatcaggt cggatcactt cacgtttttc
     1381 gatcgatatt catccagggc ggaagatttc aaaacaaatc cagcatttaa cagggatcac
     1441 gaatcaacgc gtccaaaaag cgccttattt cgaagatgtc gcccaaacga tttacaatct
     1501 tttggcagac accatatttg tcgcgcataa tatttatttt gattataatt ttttgaacca
     1561 tgaactcatg cgatgcggac ttcctagttt gaagatacca ggcatcgata cagtagaact
     1621 tgcacaaatt ttcttgccga cggaaccaag ctttcgctta gctgatttgt cagaaagcct
     1681 tggatttatt catgagaatc ctcatcaggc agatagtgat gccgaagtga cgggtcaact
     1741 tcttttgctg atcgaagaac ggatgagaaa attgccaatc atcacgttag agcagattgc
     1801 gcgattaagt agacacacag gtatggatac gagtcgtttc atttatcatg tgattgaaga
     1861 aatgaaagaa aaaccagagc cacttgatcc ctcattagaa atcgtcgatg ggctagcttt
     1921 gcagaaaaaa gaagtggaac tattcacatc cgtccattat ggcgagcgga catatccaag
     1981 aaaaaaacaa gcaaaagaaa aaatgtttgg taaaacactg atgtatcgaa aagaacagaa
     2041 cagactgatg aatgcggtct acgaccattt cacaaaagat gaatcaaaag atttgatgat
     2101 cgaagcagct acaggaatgg gtaaaacaat cggttatctg ctgccattga gttatttggc
     2161 tacacctgaa aaacccgctg tcatcagtac cgtttcgcta gttttgcagc agcagatcat
     2221 agaaaaagac attccattgc tgaatcaatt gctagatcag cccatacaac ctgtaattat
     2281 caaaagctat cgccactata ttgatttaca gcgttttaaa ggcacacttg tggaaccgcc
     2341 agaacaaaaa caatatgctt tgtatcaaat ggcggtctta gtttggctga cgcaaacgaa
     2401 gaccggtgat ttggatgaat tacatttaac aaatttgaac cattcattct ttgcagatat
     2461 cgcgcacaga ggtattggat ttttagcaag aaatcagtca ttttacgaac aagactttgt
     2521 tcgtttcttg caaaagaaaa tcaggcaaag taattttttg attgtcaatc atgcgttttt
     2581 aatacaagaa acacagcgaa aagaaccatt gcttccagct agtccatttt tgttgatcga
     2641 tgaagtacat catttgccgg aaatcgctga aaaagtaaat gacaaacgat taagtattaa
     2701 ctatttccat aaacaaatcc agcaattaga agaaccaaat cagctgtttg accgaataca
     2761 aagctattta ccgaaagaac atgaagcctt acgctggtta tcgatttatc gagaagaact
     2821 ttctgcgcta tgcgaagcac agatttgtat ggcagaagga ttaatgagtt tagtgagcgg
     2881 caagcaacct aacggacagt atccagaaga agtcatgatg acaaaagaga tgttggatca
     2941 gctgccactg gaatctggga cagccattga tcacctgcag cttttttatc aggagatcca
     3001 gcagctacag tcacaaatgc gtctgacttt tgagattatc caagaaagct ggacaaacca
     3061 gatgcggatc gatttagcaa gtttatatag cttatttgat caattagaca agcagtactt
     3121 gctgatggat caatggctgg aagactggcg agatcatttg atccattggt tcgttcctgg
     3181 gaacacagcg cagcagatga tttttcatct ccatgatttt caagcggcta tgttgccgtc
     3241 gactgtttgg tatcctcgat atgatcgtat tctttatatt ggaggaacgc tgaagatcgg
     3301 ccctaatcga cactatctct ctaagcggct tggcattgaa gcagcacctc ttaaagtgat
     3361 tgcctctcct tatgattatg agaaacaggc aaggatctat gtgccaaaag aggcacctgc
     3421 cgtccatcaa gtcagcaatc aagcatatat cgattattta gagacagcac ttacaggtct
     3481 gatcgaacaa gaagatcggc caatacttgt gttattcact tctcatgatg ttttgcagaa
     3541 ggtctatcag aaaatgcatc tgtctatgct agaaaaagga cgagaaatac tagctcaagg
     3601 gtttggcgga agccgggaga agcttttgaa acgcttcatt ttgtcagaaa aaagcgtatt
     3661 gtttggcgct gacagctttt gggaaggtgt agatttgcct ggggatgctt tgcagatcgt
     3721 tgtagtcaca cgtttaccct tcgataatcc gcttcgcccg atggtcaaag ctaggaacgc
     3781 ttatttagaa tcagagggga tcaatccttt ttaccaggaa tccgtaccta aagcagcttt
     3841 gaagttacgc caagcattag gtcgcttgat aaggacggag acagatcgtg gcgtattgct
     3901 tattttggat cgaagactag tcactgcaaa atatggtgca cggatcatcc atgcattacc
     3961 gaaaaaagta ccgatcaaag agctagctat ggaggaaatc tatcaggaca tccatgaatt
     4021 cttaaagaaa aatgaagaag gaagttagag gagctgtctt ttttcataat taaaatctgc
     4081 tataatgaaa aggaatgaag ataaaggagc gacgaccctt gaagaaaaaa cagacaaagg
     4141 aaaaaacgat taatcgcgtg ttacttggct tgatcgtatt tttattagcg gtcatcgtga
     4201 tcagctcgat tttttacttg cgttccagtc gtccggcggt gcaggcgaag aaagaagcag
     4261 tagaaatcgc aaagaaatat gcaaatcttg aggaagccga tcatttttat tggtttactc
     4321 gaaagaagac atacttttca ctgacaggta caaatgataa aggaaaagaa atcgccgtta
     4381 tcattcctaa atctggtgaa aaagtaaaag tattggatca aacaaaggga ctgactgaaa
     4441 acgaagcaaa acagacaatt gctgcggccc atcctgaaat ccaaatcgaa aaagctgctt
     4501 tagggatgta tgatgatcag cctgtgtggg aagtagtagg aaaagaaaca aatgggaaac
     4561 tgaattatta cctgattggc tttgaatctg gagatgaagt caagacaatc aagggaattt
     4621 aaaaggtaaa ggaatgaatg ggatggagtt atcaaaaaga acaaaacgat tagaaccgtc
     4681 tgtcactttg gcagctgcgg caaaggccaa ggcattaaaa gcaaaaggca gagatgtgct
     4741 gagtttgact gtgggagaac cggattttgc gacaccagaa aacatccaag aagcagcaat
     4801 cgaagcaatc cgcaatggga aagctagcta ttatacacca acagcaggaa tcccagagct
     4861 tcgacaggca atcgttgatt atctgaagaa aaactatgga cttgtctatg aaccttcaca
     4921 aacagtcgta acagacggag cgaaatttgc tttgtatacc cttttccaaa cgatccttga
     4981 cccgcaagat gaagtcatca ttcctgtgcc ttactgggtc agttacggtg agcaagtaaa
     5041 actggcagaa ggacttcctg tctttgtgaa aggggaagaa tccaatagct ggaaagttac
     5101 tgttgaacag ctagaacaag cacgttcaga aaaaacaaaa gccgtaatca tcaattctcc
     5161 atcgaatccg actggaatga tttatagcaa gaatgaactt caggcaatcg gtgaatgggc
     5221 agtaaaaaat gatattttga tagttgctga tgatatttat ggtcgtttag tttataatgg
     5281 caatgagttt actccaattg caacgatttc agaagcaatc aaaaatcaga ccatcatcat
     5341 caatggcgtg tctaaaacat atgcgatgac aggctggcgc attggttatg cggtggggaa
     5401 ttctgagatc atcaatggaa tgattgctgt tgcttcccag tcaaccagta atccgacagc
     5461 agtcagtcaa tatgcagctg ttgaagcatt gaaaggtgag caagatacag tagaagaaat
     5521 gcggaacgcc tttgaagaac gcctgaatac gttatatccg cttgtggcag aactgccagg
     5581 tgtttctctt gaaaagccgc aaggagcttt ctatcttttt cctaatgtaa aagaaacgct
     5641 acgcatgtgt aaatatgaaa atgtcacaca atgggtagaa gatcttttag aagaaacagg
     5701 ggtagcgctt gtgactggcg aaggctttgg ggcaccagaa aatgtccgca tgagttatgc
     5761 tactgatcgc gctacgttag aagaagcggt acgcagaatc aagcaattta ttgagagtaa
     5821 aagtcaaaat taatcctgaa gaaattcaag gaatataaaa ggagagtcat acgtggaaca
     5881 aattcaaatc atcgattcga aaaaccatgt gggagaaaca gtaacaatcg gcgcttgggt
     5941 cgcaaataag cgatcaagcg gaaaaatcac atttttacaa ttaagagacg gaacagcctt
     6001 tttccaaggt gtagttgtaa aaagcgaagt gtcggaagaa gttttccaaa cagctaaaaa
     6061 tctacaccaa gaaacatccg tgttagtaac tggagaaatc cgtgaagaca gtcgttcaaa
     6121 atttggttat gaaatcggaa ttacaaatgt ggaagtggta agcgagagcc acgattatcc
     6181 gatcacacca aaagaacacg gaacagactt cttgatggat catcgtcatt tatggctgcg
     6241 ttcttcaaga caacatgcga ttatgcagat cagaaatgaa atcattcgtg caacgtatga
     6301 attctttaat caacatcatt ttgtcaaaat tgatccgccg atcttgactg gttctgcacc
     6361 agaaggcact acggatctat tcgaaacaaa ttactttgat caaaaagctt atttatctca
     6421 aagtggccaa ttatacatgg aagctgctgc tatggctttc ggaaaagtct tttcattcgg
     6481 tccgactttc cgtgctgaaa aatcaaaaac acgacgtcat ttgatcgaat tctggatgat
     6541 cgaaccagaa atggctttca ctcatcaaga agaaagttta gaagtacagg aacaatatgt
     6601 agcattctta gtacaaaatg ttttagatca ttgcgaatac gcattagatg tactaggacg
     6661 agacaaagaa gtcttaaaaa cttatacaaa attgccattc cctcgtatta cttacgatga
     6721 tgcaatcgaa ttactgaaga aaaatggatt tgatgacatc gaatggggcg aagactttgg
     6781 ttcacctcat gaaacgttca ttgcgaattc attcgaacag cctgtcttta tcctaaacta
     6841 tccaaaagcc atcaagccat tctacatgaa accacatcca acaagagaag acgtggtaat
     6901 ttgtgcagat atgatcgcac cagaaggtta cggtgaaatt attggcggaa gcgaacgggc
     6961 aacggactat gatttcttgc tagaacaaat ccgtaatcac ggtttggatg aaaaagaata
     7021 ttcatggtat cttgatctac gtaaatacgg ttctgttccg cactctggtt ttggtctagg
     7081 tcttgaacga acagtcactt ggattgctgg tatcgaccat gtacgtgaag ctattccatt
     7141 cccacgtttg ttgaaccgaa tttatccata agaggttgtg cgcctgccgt tcagctccga
     7201 gtcacaagga gtaatttcaa aaaacagttt ttcatgtttg atgactattg tgacttgtgg
     7261 ccggagagca acgtttattt aaaaaaaaga actgcgaact ctcgcagttc ttttttttaa
     7321 atacgggata atggaagttt ccagcagaca agagaagaaa gcaaaagaaa ttgctatttg
     7381 attcacaagg ttctataacg aaatcgcaat caatcaaaca tttacaaaga aggtaattca
     7441 aatggaaatc tgatttgatg atgcttctgc tgttgaaata aagcagcatc tagcacctga
     7501 aaacaaacta atagtaacat tcgaagatgg agtaggtccg tattttcagc atgcaataat
     7561 ccatatgtaa gttcaattca cccttgaaat catcggacca gataaagtta tgaccaatct
     7621 atcccaagca atctagaaga gatattagta aagggacatt tactagaagt cttagatgag
     7681 cctataaata tatgtttcga tggcgtcatc agtggtctga ttgacggaag cttgggtgtg
     7741 atcgatctct ctggaccaaa caacgaagga atcaagggga atccggcacg ttaagaaaaa
     7801 aatagcataa aaaactagac acttgatgag cgttcattca attgtttgaa ggaactaggc
     7861 tggtgtcatt ttttatacat atattttctc tttcgtcttg atagggctta tgggataata
     7921 taaggaagaa aatcatcgtt ttattcaaaa aggagttttt gacaaatgaa aaaacgtata
     7981 ttatttatcg gtgatagcat tacagacgca ggaagatacc gagcagaagc aactgatcta
     8041 ggtaaaggtt atccactttt gattgccgga aaactaatct cccaatatcc aacgatggaa
     8101 tttgaatgca tgaaccgtgg gataggagga gacaaaattg cagatctgca agtacgctgg
     8161 caagaagatt gtttagcttt acaacctgac tatgtatcga ttttgatcgg aatcaatgat
     8221 acctggcata atactagcga tcaggctata ttcggaactg agaaatctgc tcagcaattt
     8281 gaatccgttt atcgaaaatt actaaatgaa cttcaaacag cggaaatctg caacatcatc
     8341 ttgatggagc cgtttgtact tcctataacc gacgaccgtc atggctggcg taaagactta
     8401 gaccctaaaa tccaaattgt tcgtaaactt gctaaggaat atggcgcatt gctggttcct
     8461 ttagatggta tattcaatgc tttagggatt gcttatggtt tttcgaaata tacaagagaa
     8521 gatggggtcc acccaacgat tggcggacat gagatcattg cacaaagctg gatagatgca
     8581 attgtcgcag tcgatttttt tagtggagat aaaaatgagc gaaagaaaag tgaaaaatga
     8641 tggaaataag gtgggcagta aaagaagatt tacctcaaat caatcagctg attactgatc
     8701 atccagaatc attcgtaaaa gcaaaaacgg cagctgacct aaatgataag aataaactat
     8761 tgctcgttgc tgtaacaaaa gaaaaacgga ttatcggatt tgcgcaagcg gacgtgcaac
     8821 agtcagaaga acgattggat cagcgaccat caggaaaact aatccaacac acattagtaa
     8881 caaaagaaaa tacggaagaa atccaaaaaa aatttgcaga atacttgatg ctggaagcta
     8941 aaaaaagaag aataaaatca atttttgccc agttttaatc aaaaacagta atagaaggag
     9001 aaatacaatg agttgtttag gaaatgtcat ctggtttata ttcggcggac taatcggtgg
     9061 tcttagctgg ttagcagcgg ggatactttg gtgtatcacg atcataggta tcccaatcgg
     9121 tatgcaatgt tttaagatcg cctccctttc ttttgcgcct tttggcaaag aagtaattta
     9181 tcaccagtct ggcatgtcat tgatcgttaa tatcatctgg ctgatcgttt caggcctacc
     9241 aattgctctc gcacatgtcg tcagcggcat ccttctatgt atcacgatca tagggatacc
     9301 ttttgccaag caatctttca aacttgctcg attggcgttg atgccattcg gtgcagaaat
     9361 tgtataaaag agaataataa aggttagata aagcatagag ccaggtggga tacatgtatt
     9421 tcacctgact ctatgcgttt tcctgtataa gttcttctat gtgactttta cgtcttttat
     9481 ttatatgggg ttactgttta tggaaagttg aaataatttt cattaaagaa ctctttctat
     9541 atttttgaaa gcgtagtgga gcttcgtata tctgggaagg acattttgca gggattgaaa
     9601 aaagatattg tcctatcgaa aaattaatga atttagacaa attgccagca acaggcttca
     9661 aacttagttg ctatcctgtc aagatcaaga aggcaagtgc gggttggata agagcagtgg
     9721 caatgatcga agagaaaaag aaagaataag ccaaaacatc tgtatggaag aaattttccg
     9781 atgcagatgt ttttcatttt ttctttcgga taccttgaca tatgaataac cattcattta
     9841 taattagtta tgaagagata ttcatatgtt gaggcgagga ggtcattaaa atgaaaagtt
     9901 acagattaga aggtttagat tgtgcgaact gtgcaatgaa aatcgaaaaa ggagttcaga
     9961 aaatcaatgg agtcaaggaa gcaacggtaa acttcacttc tggaaaacta acgattgacg
    10021 cagaggaaga tcatttggct acgattgaac aagaaacaaa aaaagtagtg aaggaattag
    10081 aaccagatgt caaagtaaca gaaatagata aggaaaaagt atctgaacat ggaaatgaaa
    10141 aaaagagaaa tacgctgttc cggattttat tttcccttgc aggtatcgct ttattgctat
    10201 tatttgattt taatgaacca attcgcttga tagggtattt attgatttat ctgctgatag
    10261 gatatgatgt agtgaagaag gcagtgatga atatcgtcaa aggaaaaatc tttgatgaga
    10321 attttcttat gtctgttgct acgattggcg ctatgttgat cggtgaatat ccagaagccg
    10381 tagcagtgat gctattttat caaatcggcg agtatttcca aggattggca gtcagtcatt
    10441 caagaaaatc gattcgcgaa ttgatggcga tccgtccaga aacagctcac gtgcagacgg
    10501 cggaaggatt gatgaccgtg aaccctgaag acgtgctgat cggtcagttt gtacttgtga
    10561 agcctggtga acgtgtaccg ttagatggaa cgatcatcga aggagaatca ttagtcgata
    10621 cgtcagcact tacaggagaa tcagtcccta aatctgtata tgttggtgaa acagtattaa
    10681 gcggatttat caataaaaat aaaccatttt tagttcaagt agaaaaatct tatgaaaatt
    10741 ctactatcag caaattatta gaattagtag aaaatgccag cagtaaaaaa gcaccagctg
    10801 aaaatttcat tactaaattc gcgcgctatt acacaccagt cgttgtagga ttggccgttt
    10861 tattagctgt tttacctcca cttgttgttt ctagagcggc gttttctgaa tggatctatc
    10921 gagcattgac atttttagtg atctcatgtc catgtgcatt agtgatttcc gtgccactta
    10981 gtttctttgg cggtatcgga ggagcaagta aaatcggtgt attagtaaaa ggttcaaatt
    11041 acttggagtt gcttgcacaa actgagacag tggtattcga taaaactggc acattaacga
    11101 aaggtgactt ttcgatccag acgatcgatc caacaggaat tgatccaaag aaatttatgc
    11161 aatatgttgc aagtgcagaa cagttttcta cacatccgat tgctcaatct gttttagaag
    11221 gctacgcagg tccgctttta ccaacagcta atatccagga atttgcaggt gaagggatct
    11281 tagctgaagt tgatggcaaa caagtattag taggtaatca caaattgatg gaaagatttg
    11341 aaatctcttt tccttcaagt caagaaatcg gtacattgct ttatctagcg atcgatcaaa
    11401 gttacagtgg ttatcttgtt attgcagata ccttgaaaga agatgccgta gacgcattgg
    11461 ttcaattaaa acaggctggt gtgaaaaaca cagtcatgtt gacgggcgac tcgaaaaaaa
    11521 ttgctgatca tatcgggaag caagtaggcg tggacaagat atatagcgaa ttgcttccag
    11581 aagataaagt gcagcggtta gaggaaatct tacaaagtaa caataaaaag accgcatttg
    11641 tcggagatgg aatcaacgat gcaccggtgc tagcaagagc agatgtagga atcgcaatgg
    11701 gtggattggg aagcgatgct gcaattgaag cagcagatgt agtcatcatg aatgatcaac
    11761 catcgaagat tgcagaagca atccatttag caaagaaaac attgaaaatc gtcaaacaga
    11821 acattgtctt tgctattggg ataaaaattt tggtactttc cttaggtgct tttggttttg
    11881 catcaatggg agatgcagta tttgcagatg ttggggtgac agtattagct gttttgaatg
    11941 ccatgcgaag cttgcatgta aaaaataaac gttgatcaat gaagaggacg aatgaacgtc
    12001 ctcttttttt tatatccttt tcttaatgaa aaatgtaaag aaaatagata ggattataaa
    12061 ataactaatg tttttaattt tgcagaaaat tcaaaatagc ttgaaattca ataaggtcaa
    12121 tgaaataaaa gaaaataact tatttttttg atggaaaaaa tatgaaaaaa cgatgtgtat
    12181 taaataaaag aatacacaca taaaataaca ctgtcttgta agtttgttaa aaattgaata
    12241 tgattacatc tggataacaa atttttatca taaaggagag aacgaatgaa caacaaacaa
    12301 ttacgctggt ttactgttgc gttaattgca tttaacatgg tttggggcat ggggaatgta
    12361 gttaataact atgctcagca aggagtatca gttgtcacct cttggctatt gattttggcc
    12421 atttatttta ttccatacgc attgatcgtt ggacaattag gatcagcctt caaggatagt
    12481 aaaggaggcg tcagttcatg ggtcgaaaat acgacatcta ataaacgact ggcatactat
    12541 gccgcttgga cttactgggt cgtccatatt ccttatttgg cacaaaagcc tcaagcaatt
    12601 ctgattgctg gtggttgggc agtccaagga aatggaaata tcgtaaatac gatgtcagta
    12661 caaatggtag cagttatttc acttattata tttcttgcct ttttatattt atcaacaaaa
    12721 ggactttcta ctttgaaagt gattggcgga ctggccggaa cagcgatgtt cgtcatgtcg
    12781 atcttattta ttcttttagc tgtaacggca ccgttcatca atccaacgat ggaatttgcc
    12841 acaccacata tgaatcaagt gaaaacatat attccaacat ttgatttcag ttatttcaca
    12901 accgtttcga tgctagtatt tgcagttgga ggggcggaaa aaatatcccc ttatgtcaac
    12961 tctacaagaa atcctgcaaa ggaatttcct aaagggatga tcttcttagc agcaatggta
    13021 gggatttgtg ctgtattagg ctcagtcgca atgggtatgc tgtttgctag cgataatatc
    13081 cctaaagact tgatggcaaa tggggcatac agtgcttttc aaattttagg taattactat
    13141 ggtatcggta actcattgat gatcatttac gcattgacaa acatgattgg acaaatctcc
    13201 gcattagcct tttcaattga tgcaccacta aaaatcttgt tagcagatgc ggatccagaa
    13261 tttgtaccaa actggctacg taaacgtaat cgtaaaggta cattggtgaa tggttatctt
    13321 ctgacaggta tactagtagg aatcattatt ttgttgccga tctttggtat caaagatatg
    13381 aatgaattag taaaatggtt gacgaacttg aactcagttg tcatgccaat gcgttaccta
    13441 tgggtgttct tggcatatat gatgttgaat aaagcttata agaaattcaa ttctgaatat
    13501 aaatttgtta aaaatccaaa aatcggtttt atctttggtg cttggtgctt tgccttcact
    13561 gcgtttgcat gtatcctagg aatggtgcca aaagtagatt atactgctga tcctaaagca
    13621 tggtggttcc aattattcac gaatatcatc acaccaattg tcttgatctt attaggtatg
    13681 atcttgccag caattgctcg tcgagacaaa aacaaagaaa ttactgaacc agaaacagtg
    13741 gttcagcctt aaaatagaaa tacttgttac aagaggttgt gacagaagct acttctggaa
    13801 caccgtgaat cagaggttgt gaaaaagctg tcctgcatca agtaataaga acagccaaac
    13861 aaaaatagct cctcatattt tgccgttttt tgagctaatg ctgaaggtca gctgattgaa
    13921 caccatttat tcgaaacaag ggactttgac acgacttttg tcacagtccc ttgtt
//
LOCUS       DACGLE010000067        13945 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0534
            SAMEA3304449-rid9560453.denovo.071, whole genome shotgun sequence.
ACCESSION   DACGLE010000067 DACGLE010000000
VERSION     DACGLE010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304449
            Sequence Read Archive: ERR985064
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13945)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13945)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702225.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702225.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 20:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,045
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 27 of 67
            Pseudo Genes (incomplete)         :: 25 of 67
            Pseudo Genes (internal stop)      :: 19 of 67
            Pseudo Genes (multiple problems)  :: 4 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13945
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304449-rid9560453.denovo.071"
                     /strain="VREN0534"
                     /db_xref="taxon:1352"
     gene            949..1422
                     /locus_tag="IXT20_002151"
     CDS             949..1422
                     /locus_tag="IXT20_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HAQ4860586.1"
                     /translation="MKFKTLLLKFVVLGISAVFILFGVLFYTQIATSEKGFNFDWQTT
                     LFVSVIYFALLLGIVIAYFLLKLLKKIEDNQAFSTQSLNILKKIERTILFIGFAFFGL
                     LPKFMKVADLDDAPGLMLVGIAVVFIPFAVYTLTSVLEKLLEHAILLKTDHDLTV"
     gene            1431..1658
                     /locus_tag="IXT20_002152"
     CDS             1431..1658
                     /locus_tag="IXT20_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ4860587.1"
                     /translation="MAIIVNLDVVLAQKKMSVTELSEKVGITMANISILKNGKAKAIR
                     FSTLEKICQVLECQPGDLLTYKNDSNTIPEE"
     gene            2294..2665
                     /locus_tag="IXT20_002153"
     CDS             2294..2665
                     /locus_tag="IXT20_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4860588.1"
                     /translation="MPFIVYFFLSLLVIYIPLPTFFLLVNRLAEQHDTIAALTKLCLL
                     LFVCLDYFTSYYWLKQTRVKKRYYFFLVFFNLFEFLIHCFLLHQYHTIHLWIIWVFKV
                     VVLFCMFTFPISRKFREALFY"
     gene            complement(2921..3163)
                     /locus_tag="IXT20_002154"
     CDS             complement(2921..3163)
                     /locus_tag="IXT20_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAQ4860589.1"
                     /translation="MGFIWSLIVGGIIGAIAGAITNKGSSMGIIANVVAGLVGSALGQ
                     ALLGSWGPSIAGMAIVPSLIGAIILVLIVSFFARKL"
     gene            complement(3195..4097)
                     /locus_tag="IXT20_002155"
     CDS             complement(3195..4097)
                     /locus_tag="IXT20_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HAQ4860590.1"
                     /translation="MNNNNKNNNNKMNNQKPVSAHPDPTTPNQSNGVNDEKNKTAPVS
                     AHPDPVVPNVQQGVEEKKHDAEKHAPHHPGTPESKVPEQHDAIDPSKPVEEQKHPSDH
                     AKAQEKLSSDHTNGQGKLGSDHTNNQGKHDSEQEDSMRGELNYEDKVVQKIIGIAIEQ
                     VDGLLSANGGFFSNVAGKLVNTDNVTAGIETEVGKKQVAVDMDVIVEYGKDIEKIFEE
                     MQEVIGKEVKNMTHLEVIEVNANVVDIKTKEEFEKDQETVQDKVTDAAKKTGEFASNQ
                     TDKVKSAAGSGAQKVKENTEPRVQ"
     gene            complement(4110..4298)
                     /locus_tag="IXT20_002156"
     CDS             complement(4110..4298)
                     /locus_tag="IXT20_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HAQ4860591.1"
                     /translation="MKELFSTYKLPIIFGVLGLILAVLWITVGFFKTLLILIMAAIGV
                     AIGFYLKQTKILDHYFKS"
     gene            complement(4312..4875)
                     /gene="amaP"
                     /locus_tag="IXT20_002157"
     CDS             complement(4312..4875)
                     /gene="amaP"
                     /locus_tag="IXT20_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HAQ4860592.1"
                     /translation="MNKGVKTIGVIGTLLLLSVLLFAILLSATYPLPFQLEKFRFFSI
                     TNSYVQQYVFWLAVAFAILTIITLLVFLFYPKTIGNFLLKHGDGELTLDKKAIEGMVR
                     SHLHEDEFIHSPKVNVKATKNKIAVNVKGELKRTSALIGKTGTLMKEIENEVQHILGT
                     KEPVKVSVKYTGFEEPAKRSNDRKRVE"
     gene            complement(4913..5806)
                     /locus_tag="IXT20_002158"
     CDS             complement(4913..5806)
                     /locus_tag="IXT20_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAQ4860593.1"
                     /translation="MTEPKLIDPRNLYHTEKFPKQDQDTPALQQNMIPVPDCGEESYE
                     GNNQLENRRVLITGGDSGIGRAAAIAFAREGADIALQFFPGEEKDAEEVAKYVKVAGR
                     KIVLLPFDLRDEQAPKEIIDKAVSELGGLDTMVLNAGQQISCQSIAELPLEQVKDTFM
                     INIIAMFALVKEAVPHIPAGGAIVTTTSVQAFNPSEHLLDYAATKASIANFTIGLAKQ
                     LAPKGIRVNGVAPGPIWTPLQLDEGQPSDSIPEFGQQSLLERAGQPGELAPVYVFLAS
                     NKASYVTAQIYGVTGGEAINL"
     gene            complement(5884..6822)
                     /locus_tag="IXT20_002159"
     CDS             complement(5884..6822)
                     /locus_tag="IXT20_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HAQ4860594.1"
                     /translation="MRYYFVDEEKNVIETDEKKYNWLVLDSAKPEEIEQAIEEFELPD
                     DIFVGTEYPEEVSRMERLYETKLEHPFSLVVINLDQSDNRIEKKLTPISFVLSDNLLI
                     TCIDRKTEFVDRLLTKHGNHLNSFEKIITYTLLDIYTHYVKELREMKKRIDALDKAAR
                     KTTENEELYKQADLERDIVYIDHTLRDQRETMDQLWNSETFIKRLDDEQLLYDVHLRQ
                     RQTEKMIEIYRDLLESIGDLFNGMMDNNLNHIMKYLDSAALIISVPALFAGIWGMNTG
                     GLPGKSSALGFILVVFGSLIAAVVLGYHLYRKDYTN"
     gene            complement(6856..7206)
                     /locus_tag="IXT20_002160"
     CDS             complement(6856..7206)
                     /locus_tag="IXT20_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HAQ4860595.1"
                     /translation="MEITMSKNAVETLIEKVGENTKIALALINDSDPFLRDKGAFAKG
                     SFFQIIPFVSEFGEYATKIEHPLLDIYTSKLEQNYFGKRLNMDFNKQLDSFSLENEIA
                     VLDYNIKLKNCFFS"
     gene            complement(7239..8141)
                     /locus_tag="IXT20_002161"
     CDS             complement(7239..8141)
                     /locus_tag="IXT20_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HAQ4860596.1"
                     /translation="MNKKEIAVVASCNTKFVPHLAALFVSVLDNCNPSKFVRFYVIDD
                     DIDFESKQLLRFSVKNARMNSDVEFLKINKEFFTNVVISDRIPETAYYRIAIPELFRG
                     TEVERILYMDCDMIALQDISKLWRLDFGDSIVAAVEDAGFHQRLEKMKISAKSMRYFN
                     SGLMLINVKKWLDENITQKVLDFIEHNPEKLRFHDQDALNAILHDRWLPLHPRWNAQG
                     YIMAKAKKHPTAAGEREYEETRNNPYIIHFSGHVKPWSKDFEGPTKKYYEKYAGMTAF
                     RCVAKFPKYPKYAKVQPKTDVVVK"
     gene            complement(8134..8991)
                     /locus_tag="IXT20_002162"
     CDS             complement(8134..8991)
                     /locus_tag="IXT20_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HAQ4860597.1"
                     /translation="MEKRYGVVPVVTASDENYAPYLSVMIATALENCNKTRRIKFYVI
                     DDGLSEYSKEGLEETVNKYSSNASIQFLTVEKDIYEDFLVSDHITTTAYLRISLPNLL
                     AKEDYKKVLYLDSDVLVLDDIVKLYDEPLNGKTIGAIIDPGQVKALERLGIDSDDLYF
                     NSGVMVIDIDQWNKKEITEKTIHYLSENGDRIIYHDQDALNAVLYEDWEQLHPKWNMQ
                     TSLIFERHPAPNEKYERQYKEGNEKPSIVHFTGHDKPWNTLKDHPYTNLYLKKLAHST
                     LTKVGEINE"
     gene            9325..10134
                     /locus_tag="IXT20_002163"
     CDS             9325..10134
                     /locus_tag="IXT20_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAQ4860598.1"
                     /translation="MKLAAIDLDGTLLDRNGKIPEENIQALRKFDENGGIVTVATGRN
                     SISAKDIFSELGIGGYLISSNGSLIAEMKDGKMAKVLRRSKIDSDTLRKAFFYAQEAK
                     ISIIASRETQDDQLTFDESNLVKDDPYYEHFNLQNHSFDQILNMLEDPSLSYLKLALT
                     DKSEEKLQKLQRDLQADGIQSVFSDPHFLEITPTGITKAHSLVFLADYLGIESSDIMA
                     FGDQENDIAMLNYSGLSIAMGNAQRQVKEIANQVTLTNEEAGVAYILKNFY"
     gene            10174..10671
                     /locus_tag="IXT20_002164"
     CDS             10174..10671
                     /locus_tag="IXT20_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAQ4860599.1"
                     /translation="MLVIIDMQNHILDPNSEFYIPESDQLAERIAQRLAKARQNNEYI
                     LFTRDIPVELKDKEEEREDLQLIPLLSPQENERVIKKYYFTIPPETLAEIQETLFESK
                     EEQKEIEVVGIETNLCVLSNLIGLQSAFPEADFFVDPMLVSSRKHGESALELLKDFNV
                     SIVES"
     gene            complement(11056..11129)
                     /locus_tag="IXT20_002165"
     tRNA            complement(11056..11129)
                     /locus_tag="IXT20_002165"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11093..11095),aa:Ile,seq:cat)
     gene            11316..11639
                     /locus_tag="IXT20_002166"
     CDS             11316..11639
                     /locus_tag="IXT20_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4860600.1"
                     /translation="MLGVIILPFIAILFDLVAAAAYFLQLNYQTSGILFIGMIFQGVI
                     TLLLLVMMLSYKGKRYARIQTKVFVKYVSIRYGIIILSFLVNAIVLFLYVLNYLDINP
                     LIFSR"
     gene            11803..12057
                     /locus_tag="IXT20_002167"
     CDS             11803..12057
                     /locus_tag="IXT20_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4860601.1"
                     /translation="MITLVRVLFWVPAVALVASIVYLMNWNKERFYLAILTLPAIYFM
                     WKVFNYNYFEPDSVFIEELSGLVLSLLIVILYLIRLNKKH"
     gene            12127..12372
                     /locus_tag="IXT20_002168"
     CDS             12127..12372
                     /locus_tag="IXT20_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ4860602.1"
                     /translation="MSIEEAILLDTLRCRLNFLRTGRSLSQVAVSSALSIPLFKYRKY
                     EEGEEIPSIIELIYIADFYDISVDYLIGRSETASKLK"
     gene            12448..12813
                     /locus_tag="IXT20_002169"
     CDS             12448..12813
                     /locus_tag="IXT20_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4860603.1"
                     /translation="MRLWHEKLISKLPRQQLLGQHRECCALRGNGWGKKHATVDYVFH
                     YSPYRLFQYHQLVMDEMEKRGYSPAPEWKNPMYRGKSCEAFFDLPTVERNVPIYPEHD
                     EVYLAECLANLEQKGIYLT"
     gene            complement(12874..13437)
                     /locus_tag="IXT20_002170"
     CDS             complement(12874..13437)
                     /locus_tag="IXT20_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4860604.1"
                     /translation="MLYIKKGIIVISFFKEYLEIINFRLKTKKESVIILVYFQGGNLM
                     IKKLFLLSILTLAMAGCSDSGLATELITKSTEATTEELKCGNDHSDYRGLNRRCMGIL
                     GRSNCKHRCSNRRRCRRSNRKIKRKIKRSNLCCASYTDVEDAVLLNHVRRCSFMSREV
                     GDEPKLEYAFSFAREDEDNPKTYDILA"
BASE COUNT         4090 a         2577 c         2304 g         4974 t
ORIGIN      
        1 ttttcggaga attaaaaaat acaataaata actttagtta ctttaatgcg ttcttacttt
       61 atttttatat tatcattttt cttattcact tctttggttt atactattct tataaataaa
      121 aacgaggtat gtcctatgat gaaaagattg tactattcat tgatcataac gattggctat
      181 ttgattgtat caaatctagg aaatatggta tttggtatca gtaaagaatt cagttggact
      241 actacattat gggaatctct attctttttc atcttcgtat ttttgttaca aaattatcga
      301 aaaaaataaa catgtgccat ctatctgttg gcacatgttt taggatttat ggtacttttt
      361 attcggcaat ccattttatt tttctacttc ttcttaatac ctctaacact tttgatttat
      421 aaactaatcc aatgcatcta cttgcgctta ttaagctatt tcagctagat agtaccaccc
      481 catgcgtcaa tgcttgattt tccttttgta taacattgta taagcaataa aggacaaata
      541 agtggtgatt acaccaaacc attgaataag aaaaattctg atacgtaaat aaacatgaac
      601 ttgaaaaaat taatgaatcg gttagctact acgtagttgt cttttttctc atacagttct
      661 gttaacttac acttctcttc gctagataat aaaaaatcct ccaactgtat tgtccaatat
      721 aatcccaggc atctaaacta gcattataga agagacacac atagaatcta aaataactgg
      781 ttttaaatgt ttcttacaat caaaaaaagc tgttaaatgc actccacacc ttcatactct
      841 atcatttttt ataaacgata tatttttatt gtttttcgat aaaaaatagt tgttttacaa
      901 taattattct gctatatttt agctatcaaa gataaaagag ggataaaaat gaaatttaaa
      961 acattgctat taaaatttgt cgttttaggt ataagtgccg tatttatttt gttcggcgta
     1021 cttttttata ctcagatcgc tacaagcgaa aaaggattca attttgattg gcagaccaca
     1081 ttatttgttt ctgtcattta ctttgccctt ctcctaggga tcgttattgc ttatttctta
     1141 ttgaaactat tgaaaaaaat cgaagataac caagcttttt ccactcaaag cttaaacata
     1201 ctaaaaaaaa tcgaacgaac cattttgttt attggtttcg ccttttttgg cttacttcct
     1261 aaatttatga aagtagcgga tttagacgat gctccaggtt tgatgttggt aggaatcgct
     1321 gtcgtattca ttccttttgc agtatacaca ttaacaagcg tgctggaaaa actgcttgaa
     1381 catgcgattt tacttaagac agatcatgat ttgacagtgt gaggtgcgac atggcaatta
     1441 tcgttaattt agatgtagta ttagctcaaa aaaaaatgag tgtgactgaa ttgagtgaga
     1501 aagtgggcat tacgatggca aacatctcta ttctaaaaaa tggaaaagca aaagccatcc
     1561 gtttttccac tctcgaaaaa atctgccaag ttttggagtg tcaaccaggt gatttgttga
     1621 cctataaaaa tgactcgaat actataccag aagaataaaa taaagtagaa caaacagtaa
     1681 ataaaaaata atctgaatga tagatgtgtg agacttttag aagttattta cgtctattat
     1741 tcagattttt tatttcgatt gaataccgtt ttatctataa ataagatatg tactgactca
     1801 tacaaagggg gccttttctt ctccatgtac tatcactttt cctgatacaa ctggtttcct
     1861 gttacgttgt agttggatct gcttttagta taatacttta ttcattccac cagactgata
     1921 gcagtttgag cagtctaaat aatcatgtag tattacaggc cattctgttt cataccttga
     1981 agtgcttaaa acttcagcta atggcccatt gtgttaactc ttactccatt cttttttagt
     2041 aagctcccat atatactgta aagccttcta tatattatgt catgttgctt tttaacattt
     2101 actatgtagt tttgataaaa tgatttgaat aacagtgatg gacaattctt ttttattttt
     2161 aacattattt tgataataat gattatttat ttaaaaatac attagataac atttgttacg
     2221 acacgaaaaa gcttcacaaa caaatatcgt tattttaaaa tgagggcgaa ctcacacaat
     2281 taggaggaat agtatgccat ttattgttta cttttttctt tcattactcg tgatctatat
     2341 tccattacca acatttttcc tgctcgtcaa tcgtctagca gagcaacatg atacgattgc
     2401 tgctcttacg aaactttgtt tacttctatt tgtttgtcta gattacttta cgagctatta
     2461 ttggctaaaa cagacaagag taaaaaagag atattatttc ttccttgtct ttttcaatct
     2521 ctttgagttt ctgatccatt gctttttact tcatcagtat catacgatcc atttgtggat
     2581 catctgggtt ttcaaagtgg tcgtattatt ttgtatgttc acctttccta tctcaagaaa
     2641 atttagggag gcattgtttt attaaagaag cttctctgta tcaataaaga cacatttaag
     2701 gaaaaacttt taaaaatcta attgggaatt tgcccgcata aataagaggc tgtgacaaaa
     2761 gtcttgtcac agcctcttat tccgtataaa cagtgttcaa aagcttgcat cttcgataat
     2821 atgcccaacc aaatgaaaaa tatgagaagc tatttttgtt tggctgttct tattacttga
     2881 tgaagaacag ctttttcacg acctcttttt aaaagtcttt ttacagtttt cgagcaaaga
     2941 atgatacgat tagtactaaa atgatggcac caatcaatga tggcacgatt gccattcctg
     3001 caatacttgg tccccaagaa cctaataatg cttgaccaag tgctgatcca actaaacctg
     3061 ctacgacatt tgcaataatc cccatcgaag aacctttatt cgtgattgct cctgcaattg
     3121 ctccgataat tcctccgacg ataagtgacc aaataaatcc catgtttcgt tcctcctaat
     3181 gttttacttc cttattactg aacccgtggt tctgtatttt cttttacttt ttgtgcgccg
     3241 cttccagctg ctgattttac tttatctgtt tgattcgaag caaattcacc ggtttttttc
     3301 gctgcgtcag ttactttgtc ttgcaccgtt tcttgatctt tttcaaattc ttcttttgtt
     3361 ttgatgtcca ctacgttagc gttgacttcg atcacttcta aatgtgtcat attttttact
     3421 tctttgccga ttacttcttg catttcttca aagatttttt cgatatcttt gccatattcc
     3481 acaatgacat ccatatcaac tgctacttgt tttttaccga cttctgtttc gatcccagcc
     3541 gttacattat ctgtattcac aagttttcct gccacattag agaagaaacc accgtttgca
     3601 gataataaac cgtctacttg ttcaattgcg attccgatga ttttttgcac aactttatct
     3661 tcataattca gctcaccgcg cattgaatct tcctgttcag aatcatgctt tccttggtta
     3721 tttgtatgat cagaaccaag ttttccttgt ccgtttgtgt ggtcagaact aagtttttct
     3781 tgtgctttcg catggtcaga aggatgtttt tgctcttcca caggtttaga aggatcgatt
     3841 gcatcatgtt gttctggaac tttagattct ggagttcctg gatgatgagg tgcatgcttt
     3901 tctgcatcat gtttcttctc ttctactcct tgttgaacgt tagggactac tggatctgga
     3961 tgtgcagata ctggtgctgt tttgttcttt tcatcgttca caccatttga ttggtttggt
     4021 gtcgttgggt ctggatgtgc agacactggt ttttggttgt tcattttatt gttattattt
     4081 ttgttattgt tattcatgga tgtttcctcc taagatttaa aatagtgatc taagattttc
     4141 gtttgtttca gataaaaacc gatagcgacg ccgatcgctg ccatgatcaa gatcaataag
     4201 gtcttgaaaa agcccacggt gatccataaa acggctaata tcaatccaag taccccaaag
     4261 atgataggga gcttgtatgt cgaaaatagt tctttcattt ttggtccccc cctattctac
     4321 tcgtttgcgg tcattgcttc gtttagctgg ttcttcaaag cctgtgtatt tcaccgatac
     4381 tttcactggc tcttttgttc ccaatatgtg ttgtacttca ttttcgattt ctttcatcaa
     4441 tgttcctgtt ttaccaatga gtgctgaagt tcgtttcaac tcccctttta cattgacggc
     4501 gatcttgttt ttcgttgctt tgacatttac ttttggtgaa tggatgaatt catcttcatg
     4561 caagtgagaa cgaaccattc cttcaattgc ttttttatcc aacgtcaact ccccatcacc
     4621 atgtttcagt agaaagtttc cgattgtttt tggataaaac aggaaaacaa gtaacgtgat
     4681 aattgtcaaa atagcaaatg caacggcaag ccaaaataca tattgttgca cgtatgaatt
     4741 agtgatggaa aagaatcgaa acttctctag ctgaaaaggc aatggatagg ttgcacttaa
     4801 tagtatcgca aacaaaagca cagatagcaa aagtaatgtt ccgatcactc cgatcgtttt
     4861 tacaccttta ttcattttcg cacctcctca taggggtgaa acgatcaact tcttataagt
     4921 tgatcgcttc tccaccagtt actccgtaaa tttgggctgt aacataactt gctttattcg
     4981 atgctaagaa aacataaact ggtgctaatt ctcctggctg acctgcacgt tctagtaaac
     5041 tttgctgacc aaattcaggg atcgaatcag aaggttgtcc ttcgtctagt tgtaatggtg
     5101 tccagattgg accaggtgct acaccattta cacggattcc tttaggtgca agctgttttg
     5161 ctaaaccgat cgtaaagtta gcgattgaag cttttgttgc tgcgtaatct aataaatgtt
     5221 cgcttggatt gaatgcttga acagaagttg ttgttacgat tgctcctcct gctgggatat
     5281 gcggaacagc ttctttcact aaagcaaaca tagcgatgat gtttatcata aatgtgtctt
     5341 tgacttgctc aagtggtaac tcagcaatag actgacaaga aatttgctgt cctgcgttta
     5401 gtaccatcgt atctagcccg ccaagttcac tcacggcttt atctatgatt tctttaggtg
     5461 cttgttcgtc acgaagatca aaaggtagta agacaatctt acgtcctgca actttcacgt
     5521 attttgctac ttcctctgca tctttttctt ctcctgggaa gaattgaaga gcgatatctg
     5581 ccccttctct agcaaatgca atcgctgctg cacgaccgat ccctgaatct ccaccagtga
     5641 ttagtacacg gcggttttct agttgattat tgccttcata ggattcttct ccacaatcag
     5701 gaacaggaat catattctgc tgtaaagctg gtgtatcctg atcttgttta ggaaatttct
     5761 ccgtatggta caagtttcgc gggtcgatta attttggttc tgtcattgct gtattcctcc
     5821 tagtttttaa attgtattgc caaaacaaat ttctttgttt gatacctgtt ttaaatagat
     5881 tctttaattt gtataatctt tccggtataa atggtaacct aaaactactg ctgctatcaa
     5941 agagccaaag actacgagta taaagcctag agcagaactt ttacccggta atcctcctgt
     6001 attcattccc caaatccctg cgaacaacgc cggaacggaa atgattaatg cagctgaatc
     6061 tagatatttc atgatatgat ttagattatt gtccatcatc ccgttgaata gatcaccaat
     6121 actctctaat aaatctcgat agatctcgat cattttttct gtttgtcgct gtctcaaatg
     6181 gacatcatag agtagctgtt catcgtctaa acgtttgatg aaagtttcag aattccataa
     6241 ttgatccatt gtttcccgtt gatctcttaa ggtatgatcg atatatacga tgtctctttc
     6301 aagatctgcc tgtttataca gttcttcatt ttccgttgtt ttacgagctg ctttatccaa
     6361 agcgtcaatt cgctttttca tctctcttag ttctttcacg taatgggtgt aaatatctaa
     6421 taatgtatac gtgatgatct tttcaaacga gtttaaatga tttccatgct ttgtcagcag
     6481 ccgatccaca aattcggttt ttctatcgat acaagtaatc aagagattat cggaaagtac
     6541 aaaagatatt ggcgttaatt ttttttcgat acgattatct gactgatcta gattaatcac
     6601 taccaaagaa aatggatgct ctagtttcgt ctcataaagg cgctccatcc gagaaacttc
     6661 ttccggatat tcggttccta cgaaaatatc atcaggaagc tcgaattctt cgattgcttg
     6721 ctcaatctct tcgggctttg cactatcaag tacgagccaa ttatattttt tttcatctgt
     6781 ttctattaca ttcttttctt catccacaaa gtagtatctc aatgtatacc tcctttttgg
     6841 actttctcga aactattagc tgaagaagca gttctttaat ttgatattat aatcgagtac
     6901 tgcgatttca ttttctaatg aaaagctgtc aagctgtttg ttgaagtcca tgttcaatcg
     6961 tttaccgaaa taattttgct ctaatttaga tgtatagatg tccaataatg gatgctcgat
     7021 tttagtcgca tattctccaa attccgaaac aaaaggaatg atttgaaaaa agctgccttt
     7081 tgcaaaagca cctttgtccc ttagaaatgg atcggaatca ttgataagcg cgagtgcgat
     7141 ttttgtattt tccccaacct tttcaattaa cgtctctact gcatttttag acattgtaat
     7201 ttccattcct actccccctc ttcagttgtt gtcttctatt atttaacaac tacatctgtt
     7261 tttggttgaa cttttgcata ttttggatat ttagggaatt tagctacaca acggaaagcc
     7321 gtcatccctg catatttttc gtaatatttc ttcgttggcc cttcaaaatc cttgctccac
     7381 ggtttcacat gtcctgaaaa atggataatg taaggattgt tgcgagtttc ttcgtattca
     7441 cgttcacctg ctgctgttgg atgttttttt gcttttgcca tgatatagcc ttgtgcattc
     7501 caacgcggat gaagtggtaa ccaacggtca tgtaagatag catttaatgc atcttggtca
     7561 tggaatcgta atttttctgg attatgttcg atgaaatcta atactttttg cgtaatattt
     7621 tcatctaacc attttttcac attgatcagc atcaaacctg agttgaaata acgcattgat
     7681 ttagctgaaa ttttcatttt ttccagtcgt tgatggaacc cagcgtcttc aacagctgct
     7741 acgattgaat caccgaagtc aagacgccat aatttagaga tatcttgtaa agcgatcatg
     7801 tcgcaatcca tatataagat acgttcaact tctgtgccgc ggaacaattc cggaatcgca
     7861 atacgataat aagctgtttc tggaatacga tcgctgatta ccacatttgt aaagaattct
     7921 ttattgattt tcaagaattc cacatctgag ttcattcgtg cgtttttcac tgagaagcgt
     7981 aaaagctgtt tactttcaaa atcgatatca tcgtcaatca catagaaccg aacaaattta
     8041 gacgggttgc aattatctaa tactgagaca aataatgcag ctaaatgtgg tacgaatttt
     8101 gtgttacaac ttgcgactac tgctatttct tttttattca ttgatctcgc ctactttcgt
     8161 tagagtactg tgtgctaatt tttttaagta taaattggta tacggatgat ctttcaacgt
     8221 attccaaggt ttatcatgtc ctgtaaagtg aacgatcgaa ggtttttcat ttccttcttt
     8281 atactgacgt tcatattttt catttggtgc tggatgacgt tcaaaaatca gcgaagtctg
     8341 catgttccat tttggatgaa gttgttccca atcttcataa agtacagcat tcaaagcatc
     8401 ttggtcgtga tagatgattc gatcgccatt ttcactgagg taatggatag ttttttctgt
     8461 aatttctttt ttattccatt gatcaatgtc aatcaccatc actcctgaat tgaagtacaa
     8521 gtcatcggaa tcgattccta atctttccaa agctttcact tggcctggat cgataatagc
     8581 gccgattgtt tttccattta acggctcatc atatagtttt acgatatcgt ctagtactaa
     8641 cacatctgaa tctaagtaaa ggactttctt ataatcctct ttagctaata agtttggtaa
     8701 agaaattctt aagtaagcgg tagttgtgat atggtcactg actaggaagt cttcgtagat
     8761 atctttttcc actgtcagga attggatact tgcattactt gaatatttat ttaccgtttc
     8821 ttccaatcct tctttactat attctgatag accatcgtca atcacataaa attttatgcg
     8881 tcttgtttta ttgcaatttt ctaatgcagt tgcgatcatc acgctcaagt aaggagcata
     8941 attttcgtct gaagcagtta ccacaggaac tacgccgtat ctcttctcca tcaaaatcac
     9001 tcctttttct aatctttttc taacctgttc tacatctaga atattcctca tttggtttta
     9061 aagcaaaata tatgctttgt tttcattgaa tattttaaaa tttttttatc acaaaacgca
     9121 tcggtaagcg ctaactattc gtttttcatg gctttttatt caaaggtatt gaccaaaaac
     9181 tactatatat agccaccttt ttcttaagaa tgttttttga aaaaattcat aatgacaaag
     9241 caagttagtt tcattttttt aaattctcat ttaatcttaa ggtaacagga aaatagatat
     9301 gtaaaaagaa agtaggaaga taaaatgaaa ctagcagcga ttgatttaga cggtaccttg
     9361 cttgatcgaa atggaaagat accagaagaa aatatccaag cacttcggaa atttgatgaa
     9421 aatggcggaa tcgtaaccgt cgctaccgga aggaacagca tttctgctaa agatattttt
     9481 tccgaattag gaatcggtgg ctatttgatt tcttccaatg gttcactgat tgctgaaatg
     9541 aaggatggaa aaatggcgaa agttctcaga cgttcaaaaa tagatagcga tacactgaga
     9601 aaagccttct tctatgctca agaagcgaaa atatccatca ttgctagccg agaaacccaa
     9661 gatgatcagc tgacattcga tgaatcaaat ctagtcaaag atgaccctta ttatgaacat
     9721 ttcaatttac agaaccattc ttttgatcaa attttaaata tgttggaaga tccttctctt
     9781 agctatctta aattagcatt aacagataag agtgaagaaa aactacagaa gctacaaaga
     9841 gatctgcaag cagatggtat ccaatcagta ttctctgatc cgcatttctt agaaatcacg
     9901 cctacaggta tcactaaagc tcattcactt gtttttctgg cggattacct tggaatcgaa
     9961 tcgagtgaca tcatggcttt tggcgatcaa gaaaacgaca ttgctatgtt gaattacagc
    10021 gggttgagta ttgcgatggg gaatgcacag cgacaagtaa aagaaattgc aaaccaagta
    10081 acactgacga atgaagaagc tggtgttgct tatattttaa aaaacttcta ttaaaaagaa
    10141 taaaatgacg atatgaatgt aggaggaaag aaaatgttag ttattattga tatgcaaaat
    10201 catattcttg atccaaacag cgaattttat attccagagt cagatcaatt ggcagaacgt
    10261 attgctcaaa gattagctaa agcacgccaa aataatgaat atattctctt cacacgggat
    10321 ataccagttg aactgaagga caaagaagaa gaacgagaag atttgcaatt gatccctctt
    10381 ctttctccac aagaaaatga acgagtgatt aaaaagtatt actttacgat tccacctgaa
    10441 actttagctg aaatccagga aactttattt gaaagcaaag aagaacaaaa agagatcgaa
    10501 gtagtcggga ttgaaacaaa cttgtgtgta ctctctaatc tgataggcct gcagagtgct
    10561 tttcctgaag cagacttttt tgttgatcca atgttagtct ccagtagaaa acacggagaa
    10621 tccgctttag agctgttgaa agatttcaac gtctctatcg tcgaatcata aaaatatcta
    10681 tcttcattta cgatatttaa cacacaataa aaaaagcacg cagaactgtt ttagtcctac
    10741 gtgctttttc tatcaaagta gattggcttt tttatgacac agagtgaaac ggcaggctca
    10801 cgacctcttt cttaaatggt gttcaatcag ctgaccctcg gtattagctt aacaaacggc
    10861 aaaatatgag gaactatttt tccgtttgtt aagctaatac tcaggtcaaa acgctgattt
    10921 cacaacctct tccttaaacg gtattcaaaa gcttgcatct gcagtaataa gaacagccaa
    10981 gtaaaaacat aagaagctgt ttttacttgg ctgttcttat tacttgatgc agggcagctt
    11041 tttcacaacc tcttatgtac cttgtaggac tcgaacctac gaccggacgg ttatgagccg
    11101 tctgctctaa ccaactgagc taaaggtaca tcataaataa aatttatttt tgttactttt
    11161 caactatagc acgaatgaca aactctcaca agtttcaaaa tataagtaaa ataatattgt
    11221 tattcttatt tcttttactt gtgtcataac tattttgtta ctttaatttt tatttgctta
    11281 ttttagaaaa aatgttattc ttttatttat tctcaatgtt aggagtgatt attttgccat
    11341 ttattgcgat tttattcgat ttagttgcgg cagcagctta ttttctccaa ttaaattatc
    11401 aaacctcagg aattctgttt attggaatga tttttcaagg tgtgattacc cttttgcttc
    11461 ttgtcatgat gctcagctat aaaggaaaaa gatatgcccg tattcaaaca aaagtatttg
    11521 ttaaatatgt ttccattcgc tatgggatca tcattctttc ctttttagtg aatgcgatcg
    11581 tcctcttttt atatgtattg aattacttag atatcaatcc attgattttt tctcgataga
    11641 taagacacat tcagcggaac agttcctgct tatcagcaga aaactgttct gctttttcat
    11701 ttttcattga tctattcttc ttttattttc ctagcatttc atttgtgatg tatgcaataa
    11761 catgttatct tttattatat gaatcgttga ggagtccgtt acatgattac gttggtgaga
    11821 gttttgtttt gggtgcctgc ggtcgcactg gtcgcaagta ttgtttatct tatgaattgg
    11881 aacaaagaac ggttttattt agctattttg actcttccgg ctatctactt tatgtggaaa
    11941 gtttttaatt acaactactt tgaacccgat agtgttttta tagaagaatt atcaggactg
    12001 gttttgtctt tattgatcgt cattctttat ttgatacgtt taaataaaaa acattgagta
    12061 ctattgattg catgtgtagc tttctatagt cattataact attctactat aatgaaaggt
    12121 gattaaatga gtatcgaaga agctatttta ctggatactt tacgttgtcg tctgaatttc
    12181 ttaagaactg gccgatcttt atctcaagta gctgtttcta gcgcgttaag tattccttta
    12241 ttcaaatatc gtaagtacga agaaggagaa gaaattccaa gcatcattga attaatctat
    12301 atcgctgact tttacgatat ttccgttgat tacctaattg gtcgctcaga aacagcaagc
    12361 aaactgaagt gattttttcc agccttggtg ctggtttttt catttaacgt ctaaggttaa
    12421 caatctatat ataaaggaga catttctatg cgattatggc atgaaaaatt gatttcaaag
    12481 cttcctcgtc aacagctttt aggacaacat cgggaatgct gtgcattaag aggaaatggc
    12541 tggggaaaga aacatgctac tgtggattat gtttttcact attctcctta tagattattc
    12601 caatatcatc agcttgtcat ggatgaaatg gaaaaacgtg gttacagccc agcaccagag
    12661 tggaaaaatc ctatgtatcg tggcaaatca tgcgaagcat tttttgattt acctactgta
    12721 gagcgcaacg tgccgattta tccggaacac gatgaagttt atctagcaga atgtttagca
    12781 aatttggagc aaaaaggaat ttatcttacc taatataact agaaaagcct tctatctcct
    12841 cttacttcat taaaaagctt taaccatctt agatcaagct aaaatgtcgt aagttttagg
    12901 gttatcttca tcctcacgtg caaaactaaa agcgtactct agttttggtt catcgccaac
    12961 ttccctagac ataaatgaac atctcctgac gtggtttaaa agtaccgcat cttcgacgtc
    13021 tgtatacgat gcacagcaca gattgcttct tttgatcttt cttttgattt ttctgttgct
    13081 tcttctacac cttcttcgat tactacatcg atgcttacaa ttgcttctgc ctaatattcc
    13141 catgcatcgt cgatttaagc ctctgtagtc actgtgatcg tttccacatt ttaactcctc
    13201 tgtcgtggct tcagttgatt ttgtgatcag ttcagttgct aagccagagt cagaacaacc
    13261 tgccattgct agcgttaata ttgatagtaa aaatagtttt tttatcataa ggttcccccc
    13321 ctgaaaatag actaaaataa taacgctttc tttttttgtt ttcagacgaa aattaataat
    13381 ttctaaatat tctttaaaaa agcttataac tattatgcct tttttaatat ataacacagt
    13441 tatttttaaa aataagaatg ctaaatcact ctacaataac ggcgaaagca caaaaaataa
    13501 aaaaattatt acgttattaa aaaaacagat gaaagatcag gttttattag atctcattct
    13561 ttcatcactt gaagggattt aatgaactac tttttcctct ttgtcgtttt tatatattcg
    13621 tattccttca aacagtagga tagcctcttt caatgcttga tatttgaact gtccgttact
    13681 taaaaattta gatgtgccgc cctcttccta gaacatattc ttttttactt tttcattaat
    13741 tgacagacca atcgaacgaa actgtaaatt tcgataggat aatgattggt caaactcatt
    13801 cattttcaag acatctcgat tcatattcaa gataggaatc tttttatcaa catcacttta
    13861 ttcgtatttt cctctaccat aaaagcttca cgctccacaa aattgtttta tacactacta
    13921 ttcagcccat tcaacgacca atgaa
//
LOCUS       DACGLE010000068        13917 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0534
            SAMEA3304449-rid9560453.denovo.072, whole genome shotgun sequence.
ACCESSION   DACGLE010000068 DACGLE010000000
VERSION     DACGLE010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304449
            Sequence Read Archive: ERR985064
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13917)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13917)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702225.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702225.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 20:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,045
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 27 of 67
            Pseudo Genes (incomplete)         :: 25 of 67
            Pseudo Genes (internal stop)      :: 19 of 67
            Pseudo Genes (multiple problems)  :: 4 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13917
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304449-rid9560453.denovo.072"
                     /strain="VREN0534"
                     /db_xref="taxon:1352"
     gene            131..505
                     /locus_tag="IXT20_002171"
     CDS             131..505
                     /locus_tag="IXT20_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HAQ4860605.1"
                     /translation="MYLKITEQAQERLQKYIDEGATVILDLDDGVGEYSKMGVCSLDT
                     SFRLLLLDKEQRKNDYKLQLDSDIGDVYIKDYSKMYMDEHMTLSLDPRLGVFTLESPS
                     GSLDSHVQLVDLRPEKFSGIKQ"
     gene            complement(619..1248)
                     /locus_tag="IXT20_002172"
     CDS             complement(619..1248)
                     /locus_tag="IXT20_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HAQ4860606.1"
                     /translation="MKIVFLHGLGQDEQSWKDVISYLPNSYSCKSLSLFNHLNSTDTA
                     TLDDLTKYIEVQLNQIKEPFILCGLSLGAVIALNYLATPSPYLKKVVLVAPQFESPNR
                     LLLTFQNIYFYCVPSKIFEKLGLSKKQTLTLLRSLETLDCKKQLRTSRLPKLILCGTK
                     DHANKPAAKRLYKLSTNGRLLFIKNGKHELNREKPEIMAQAMRSFIENS"
     gene            complement(1382..1454)
                     /locus_tag="IXT20_002173"
     tRNA            complement(1382..1454)
                     /locus_tag="IXT20_002173"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1419..1421),aa:Thr,seq:ggt)
     gene            1668..1741
                     /locus_tag="IXT20_002174"
     tRNA            1668..1741
                     /locus_tag="IXT20_002174"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1702..1704,aa:Asn,seq:gtt)
     gene            1696..1845
                     /locus_tag="IXT20_002175"
     CDS             1696..1845
                     /locus_tag="IXT20_002175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4860607.1"
                     /translation="MTVNHDVVGSSPTAGVNKAYAKKKKSNHLGFFFFYYYDESSFLF
                     LSRML"
     gene            2338..3666
                     /locus_tag="IXT20_002176"
     CDS             2338..3666
                     /locus_tag="IXT20_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HAQ4860608.1"
                     /translation="MKAITPDMTTKFNKQFQANIKQQALQRAVVKNGITASAENVSAH
                     VENTPVFSIDLATGKVANQMQSGRCWMFAALNTFRHKMLNQFHLKEFELSQNYTFFWD
                     KYEKANYFYENIIATGKEAIDSRKVAFLLATPQQDGGQWDMIVSLFQKYGVVPKSVMP
                     ESANSSNSRDLNNYLNKLLRKDAVVLRKMVAQGETLDAIEEKKEEMLESIYNFLAISL
                     GTPPKEFDFEYRDEEKNYHLDRGLTPQIFYDKYIGVKLDDYVSVINAPTKDKPFNRSY
                     TVEMLGNVVGGKEVKYLNVDMETFKKLAIAQLEEGESVWFGCDVGQSSTRTSGIMALD
                     AYSMDDLFDTDFTMTKAERLDFGESLMTHAMVLTGVDLVDGQSTKWKVENSWGEKVGK
                     NGFFVMSDAWMDEYTYQIVVRKDLLTKEQLNAFNEEPIVLSPWDPMGALA"
     gene            3868..4701
                     /locus_tag="IXT20_002177"
     CDS             3868..4701
                     /locus_tag="IXT20_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HAQ4860609.1"
                     /translation="MDIQTVLSDLKLNGKVIPVVGGDVNQTYRIKIEHRAYFLKIHPN
                     VKKGFFEAEVDGLKELSAFVRVPDTYMLGETSEGAYLLMEWIEPGKGDQRDLAAALAN
                     LHQQTAPQFGFRKDNYLGTLVQKNSFEEDWWTFFFKDRLESQISLAEETNRWNVQRQE
                     KYLRFKERVLKSVEPKKITPRLLHGDLWSGNVFFDQQGHPVFVDPAVSYGNREQDIAM
                     SQLFGGFRPEFLDAYQTIFPLEKGWKDRLPIYQLYYLLAHLNMFGESYGSQVDQLLEN
                     F"
     gene            4717..5454
                     /locus_tag="IXT20_002178"
     CDS             4717..5454
                     /locus_tag="IXT20_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-[acyl-carrier-protein] thioesterase"
                     /protein_id="HAQ4860610.1"
                     /translation="MAKKYTKKHEVSYYECDINQTMTFPSMLGVVIKTSEDQSDQLGR
                     GSEFVNSFGLTWVITNYSMEINRLPRVGEVISVTTQAMEYNKFFCYRNFWIHDEDGKE
                     LVKIESVFVLMDFVNRKMSSVNEEIIAPFESEKIKKIKRQEKIEKIDSGFMLPYRVRF
                     YDIDSNQHVNNAMYFNWIIDVLGYDFLTTHVPEKVIIRFDKEVEYGNEIESHFEQIPI
                     ENGVKTKHEIRMQEQLYFEANIIWKNK"
     gene            complement(5609..6577)
                     /locus_tag="IXT20_002179"
     CDS             complement(5609..6577)
                     /locus_tag="IXT20_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HAQ4860611.1"
                     /translation="MKKILVLHTGGTIAMKENMLTGGVSPDVANPLLDAEITIPKHVE
                     LLVEDIFNLPSPHIMPSHMLVLKERILSAYASGISGVVITHGTDTLEETAFFLDTTIG
                     NKLPIVLTGAMRSSNELGSDGLYNFESAIRVASCEEALDKGVLVVMNDEIHSARYVTK
                     THTTNVATFRTPTLGPIGLVTKNRILFLQELLETKRLDISAVDGTIPIVKAYAGMQGD
                     LLEAIAHTKVDGLVIEALGAGNLPPQALAALEKLLVQKIPVVLVSRCFNGIAEPVYDY
                     EGGGKELEKMGIIFCNSINSQKARLKLLIAVNYGLSGEELISFIQN"
     gene            6809..7642
                     /locus_tag="IXT20_002180"
     CDS             6809..7642
                     /locus_tag="IXT20_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HAQ4860612.1"
                     /translation="MIRIGLTSFKEHDKLTGKKNNTLYEYAGFLPLVELDTAYYGIPR
                     KSSVENWISEVPESFRFVVKVYQGISGQGDWKPYYQTEEEMIAHFLDVMSPMVDAGKM
                     FCFLVQFPSSFKCTKENVQYLRKLRKWFEGYPVSIELRDYSWYSDEFLERTRNFMSRA
                     AFSLAIIDEPQLLNTTIPFDPFVTNPNFALFRFHGRNAQGWQANDKEWRKKRTLYRYS
                     EKELQELAHEVKVVDSKTKEVGVIFNNNSGGDAAENALALQKILNIKPDGLNPTQMDL
                     F"
     gene            7676..8515
                     /locus_tag="IXT20_002181"
     CDS             7676..8515
                     /locus_tag="IXT20_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAQ4860613.1"
                     /translation="MIKAIFFDIDGTLVTNRSKALESTKQAIEYARKNGILCGVATGR
                     SPVKIKEIIDELELDMYVVYNGQLVFTADRTIIDHPFEQKVLEHIVEFADENHRQIVF
                     GARNRLDGSTTMLLGQSIFIKRLVSFLPRKFPVRLMKKILQVFSPHRQKDRYETLEIL
                     KEPIYQCILLSPESEQKKLEHRFPECTFQRSNTYTVDIIPKGGSKLLGIQAFANAVEI
                     EMEEIMAFGDHYNDIEMLKGVGIGVAMGNAQIEVKHAADFVTQSNEEDGIYEGLKHFG
                     LIY"
     gene            8541..9101
                     /locus_tag="IXT20_002182"
     CDS             8541..9101
                     /locus_tag="IXT20_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HAQ4860614.1"
                     /translation="MEAETPFQKVETFHKTFDPRRPEIPTAFTENEAHTRAAFKIEEL
                     VEFVAATSPNKEQLHEAVMLLHEALDKAEEKILGKTQWGSDPLVEQVDALTDILYFTY
                     GSFSLLGIVPDRIFEIVHQANMGKLFPDGKPHYDPVTNKVLKPENWQMEYAPEPKIAA
                     EIQRQIQEAIAKKKSADIGMCTNEKE"
     gene            9300..11021
                     /locus_tag="IXT20_002183"
     CDS             9300..11021
                     /locus_tag="IXT20_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation ring formation regulator EzrA"
                     /protein_id="HAQ4860615.1"
                     /translation="MGNNVIIGIVIAIIIVAIVVFLVSYLMKKKNQDRLDALEKRKEE
                     LFDLPVIEEVDDVKKMHLVGQSQNTFREWSQKWNDISTSSFAELESQIFEVENLNESF
                     RFLKGKKAVVEAEATMDQMEKQVTEIREGLKELRETEERNSLAVQQALDVYEELKEKV
                     REDEHEYGPALFELQKQIKVIEKEFTQFVTLNTSGDPVEAREVLDKAEKHTYEVEALM
                     KKIPPLYEELHTTFPEQLEEISDTYEKLMEEQYVFPEEDLAEDIAKVSRRIENSLANL
                     EKTEVETVEFENRETADLIDSLYDILEREMEAQRYVKTNQSTIAEYIKHTTKNNRQLL
                     IELDHTSQSYTLNHNEIGRVRGFQTEVEEMERQNEQMIPQIRQHEIPYSEVRTFYKTV
                     FKVLEDIEKQQVEIDDSLHELRKGEKEAQEKIDTFEFKLRSLKRFVEKQRLPGLPNDY
                     LEFFFVATDRIEELSVVLNKIRVNMEEVNRLVALCEEDLELLDKKTHDLVDAAALTEQ
                     MLQYANRYRHTHEEVREAIDKSYYLFNKEYHYQEALDEIGTALERVEPGAFKRIEDFY
                     FNHPDLV"
     gene            11186..12328
                     /locus_tag="IXT20_002184"
     CDS             11186..12328
                     /locus_tag="IXT20_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HAQ4860616.1"
                     /translation="MIYFDNSATTPIFPQSLDAYVKTSQRIIGNPSSLHDLGNQANRL
                     LQQARKQIADLIHVEPEEIYFTSGGTEGDNWVLKGTMIEKREYGNHMIISGVEHPAVS
                     ETAEQLKELGIEVSIAPVDKRGFVRVDELKELIRKETVLVSVMAVNNEVGSIQPIQEI
                     SDLLDDFPKIHFHVDAVQAIGKVPFSQWLTERVDFATFSAHKFHGPRGTGFIYWKKGR
                     RLAPLLTGGGQERNQRSGTENVPGIVAMVKALRLSLEIKEQKPEQTQRLKVALINALK
                     TYEKVTIFSEGTDYAPHILCFALKGIRGEVLVHALEEKQIFVSTTSACSSRKHMASST
                     LHAMHVPNDLATSAVRISLDENNSLAEVEQFMIIFKQLYKKFSAIN"
     gene            12344..13555
                     /gene="thiI"
                     /locus_tag="IXT20_002185"
     CDS             12344..13555
                     /gene="thiI"
                     /locus_tag="IXT20_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HAQ4860617.1"
                     /translation="MKYSEIMVRYGELSTKGKNRKSFIMQLAQNVRQALKDFPEIKIH
                     ADRDRMHLLLNGADGQLVIPKLAKIFGIQNFSPSIRVEKDVEVLKQAVQDIMKEIYTG
                     KETFKIIAKRSDHQFELTSNELNQTLGNAVFDIFPHIQVQMRQPDIPLRVEIRRDGAY
                     LSYETIKGAGGLPVGTSGKGMLMLSGGIDSPVAGYFAMKRGVEIEAVHFASPPYTSEQ
                     ALQKAKDLTAKLAPYVGTIHFIEVPFTEIQEEIKKSVPQGYWMTITRRMMLRLTDEIR
                     RIRHGLVILNGESLGQVASQTLQSMVAINEVTNTPIIRPVATMDKLEIIEVAEQIDTF
                     NLSIQPFEDCCTIFAPPQPKTRPQLEKVHQYEERLAIDEMIQRALAGLKMEEIQAESS
                     NEKTEEFSELL"
BASE COUNT         4751 a         2297 c         2832 g         4037 t
ORIGIN      
        1 ctacactctg agatgtaaga aatatcataa gatatttctt acatcttttt gttttgagca
       61 ggactttaga taattttatt aatatatgct agcatgcttt tgtaagaaaa agaaagaggg
      121 cgtattagtt atgtatctta aaattacgga acaagcacaa gaaagattac aaaaatatat
      181 tgatgaaggc gcaacagtta tattggattt agatgatggc gttggggaat attctaaaat
      241 gggtgtttgc tctttagata ctagttttcg tttacttctt cttgataaag aacagagaaa
      301 aaatgattat aaactccaat tagatagtga tattggggat gtttatataa aagattattc
      361 aaaaatgtac atggatgaac atatgaccct ttctttagat ccacgtctag gtgtatttac
      421 tcttgaaagc ccaagtggtt ctttagacag tcatgttcaa ttagtagatc tacgaccaga
      481 aaaattctct ggaataaaac aataactttc tctctattgt ccataaaact agtctcaatc
      541 aagctgtatt gaaaaaacaa gacaacattc ttaccatggt aaataaaaag acgacacatt
      601 ttgtcgtctt tttttaaatt aactattttc gataaaagat cgcatagctt gtgccattat
      661 ttctggcttt tctctattca attcatgttt tccgttttta ataaagagta gccggccatt
      721 cgttgaaagt ttatataaac gttttgctgc tggtttattc gcatgatctt tggtaccgca
      781 taaaatcaat ttaggaagtc gtgatgtacg aagctgtttt ttgcagtcta atgtttcaag
      841 tgaacgaagt agggtaaggg tttgtttttt agatagacct aatttttcaa atatcttaga
      901 aggtacgcag taaaaataaa tattctggaa agtaagcaat aagcgattag gagactcaaa
      961 ttgtggagcg actaaaacaa ccttttttaa atatggagaa ggagttgcaa gatagtttaa
     1021 agcaataaca gcgcctaaag ataatccaca taaaataaaa ggttccttta tctgattcaa
     1081 ttgtacttcg atgtatttgg ttaagtcgtc taaagtagca gtgtctgtgc tgtttaaatg
     1141 attgaacaat gaaagacttt tgcaagaata actattgggt agataagaga ttacatcttt
     1201 ccatgattgt tcgtcttggc ctagaccatg caaaaaaaca attttcatat tgctctcctt
     1261 tttatagtta tcatatagac tttctttgta ctattaaaat ataaacattc atttcttacg
     1321 tcataaaata aaaaaacagc gcagacacat tgttaaacca acatgttgca ctgtttaagg
     1381 aagccacttg ccgggcttga accggcgacc tcttccttac catggaagcg ctctaccagc
     1441 tgagctaaag cggcattatc attaatacaa ataataggat aacgaatatt aatgtatttg
     1501 tcaacagaat tttttcgaaa aaatgtgttt ctctaatagg aaaacagatt ttagaaaaaa
     1561 taaaaataac aaaaaatgcg aacggtttct ttgataatat aataaaaaaa ttcaaaaaaa
     1621 gtattgcgtt atttagaaga aattgatatt atattcatgt acttgattcc ggcatagctc
     1681 agttggtagt agcgcatgac tgttaatcat gatgtcgtag gttcgagtcc tactgccgga
     1741 gtaaataaag cttatgcaaa gaaaaagaaa tccaatcact tgggtttctt tttcttttat
     1801 tattatgatg aatcaagctt tctgtttttg tcacgtatgt tgtaatcttt acgaaagagt
     1861 agagttggct actcataatc aaccatactt cccatcatcg ctaaatacga aataaatcag
     1921 cattcctaca ccaatcaaag cacaagctgc accaagaaaa gggatgacat tcccaaagtt
     1981 atctgtattc ataagtatgc caccacccac aaacaaaaag ccgccagcaa tcaggttttt
     2041 cattttattc atctcctttc ttacgttata gtatttcctt ctttcaatga gtattttgaa
     2101 agctatctta ttgtatgatg ttcttctagg cctcttcttt attatacagg ccattttcgt
     2161 tatctcatag aaatattcct ttttatctga aaactattgt ataccgtatg acatttttct
     2221 tcatgaagtt gtaagtatat tctcattcaa atgaaaattt catcatagaa gagattgtat
     2281 ttctaaattc ttaagagtaa aatacgaagt ataacttgga tttggaagga tgattggatg
     2341 aaagctatta cgcctgatat gacaacaaaa tttaacaaac agtttcaagc aaatatcaaa
     2401 cagcaggcat tgcagcgggc agttgtgaag aacggaatta ctgcttctgc tgaaaatgtt
     2461 tcagcacatg tggagaatac accggtattt tctatagatt tggctacggg gaaagtggct
     2521 aatcaaatgc aatcggggcg ttgctggatg tttgctgcac tgaacacatt ccgtcataag
     2581 atgctgaatc aattccattt aaaagagttt gaattatcac aaaattacac ttttttctgg
     2641 gataaatatg aaaaggcaaa ttatttctat gaaaacatca ttgcaacagg aaaagaggca
     2701 atcgatagcc ggaaagtagc tttcttactc gctactcctc aacaagatgg cggccaatgg
     2761 gatatgatcg tttcattatt ccaaaaatat ggcgttgtgc ctaaatcggt gatgccggaa
     2821 agtgcgaata gttcaaattc tcgagatttg aataactact tgaacaaatt actgcgtaaa
     2881 gatgcagtag tgttacggaa aatggttgct caaggagaaa cattagacgc tattgaagaa
     2941 aagaaagaag aaatgctaga aagcatttat aacttcttag ctatctcatt aggaacacct
     3001 ccaaaagaat tcgattttga gtaccgcgat gaagaaaaga actatcatct tgaccgagga
     3061 ctgacaccac aaatttttta tgataaatac atcggtgtaa aattagatga ttatgttagc
     3121 gtgatcaatg caccaacaaa agacaaacca ttcaatcgtt cttatacagt agagatgcta
     3181 ggaaatgtcg ttggcgggaa agaagtcaaa tatctgaatg ttgatatgga aactttcaaa
     3241 aaattagcga ttgctcagct tgaagaagga gaatctgttt ggtttggttg tgatgtaggt
     3301 caatcttcta ctagaactag tggcatcatg gccttagatg cttattcaat ggatgatcta
     3361 tttgatacgg attttacaat gactaaagct gaacgtttag attttggaga aagtctgatg
     3421 acccatgcga tggtattgac cggtgttgac ttagtagacg ggcaatcaac aaaatggaaa
     3481 gtcgaaaata gctggggcga aaaagttgga aagaatggct tctttgttat gagtgatgcc
     3541 tggatggacg aatatacgta ccaaatcgtt gtaagaaaag atttattgac gaaagagcaa
     3601 ttaaatgcgt tcaatgaaga accaattgtt ctatctccgt gggatccaat gggtgcttta
     3661 gcatagacga gtatttcaat gaagagtatg tagtaaagat aaaaagagct ggcttaagcc
     3721 agctcttttt atctttaaag tccattccct gttttttatg gtccctgtgc tataattcta
     3781 aataaaggat aaggattgga aataatcctt ttatcattaa aaatatgatg acgaagtaga
     3841 ataaaattgg gagtaggggg gaaagtcatg gatatccaaa ctgttttatc tgatctgaaa
     3901 ttaaatggta aagtgattcc agttgttggt ggcgatgtta atcagaccta tcgtataaaa
     3961 atcgaacatc gtgcttattt tttaaaaatc catccgaatg taaaaaaagg attttttgaa
     4021 gcagaagtag acggattaaa agaactgtca gcattcgtac gtgtaccgga tacgtatatg
     4081 ctaggtgaaa caagtgaagg cgcttatttg ttaatggaat ggatcgagcc gggaaaaggg
     4141 gatcaacgag atcttgctgc agctttagct aatctccatc agcaaacagc gccacaattt
     4201 ggttttcgta aagataacta tttgggtaca cttgttcaga aaaattcttt tgaagaagat
     4261 tggtggactt ttttctttaa agatcggctt gaatcccaga tttctttggc agaagaaacg
     4321 aatcgatgga atgtacagcg tcaagaaaag tatctgcgat ttaaagaacg tgtattgaaa
     4381 tcggttgaac caaaaaagat cactccacgc ctcttgcacg gagacctgtg gagtggtaat
     4441 gtgttctttg accagcaagg gcatccagtt tttgtcgacc cagctgtttc ttatgggaat
     4501 cgggaacaag atattgcaat gagccagtta ttcggtggtt tccgaccgga atttttagat
     4561 gcttatcaaa ctatatttcc attagaaaag ggatggaaag accgactgcc gatttaccag
     4621 ttatattatc ttttggcaca tttgaatatg tttggagaaa gttacggttc ccaagtagat
     4681 caattactag aaaattttta agaggagggc gtgtttttgg caaaaaaata taccaaaaag
     4741 catgaagttt catattatga gtgtgacatc aaccaaacga tgacatttcc ttcgatgtta
     4801 ggagtagtta tcaaaacgtc tgaagatcaa agcgaccagt tgggaagagg cagtgaattc
     4861 gtcaatagct ttggacttac ttgggtcatc acgaattatt ccatggagat taatcgtctc
     4921 cctagagtcg gtgaagtaat ctcagtaacg actcaagcca tggagtacaa taagtttttc
     4981 tgttatcgga acttctggat ccatgatgaa gatgggaaag aattagtcaa aatagaatct
     5041 gttttcgtat tgatggattt tgtgaatcga aaaatgagca gtgttaatga agagatcatt
     5101 gctccttttg agagtgagaa gatcaagaaa atcaagcgtc aggaaaaaat tgaaaagatt
     5161 gattcgggct tcatgttgcc atatcgtgtt cgtttttatg atatcgatag taatcagcat
     5221 gttaataatg cgatgtattt taattggatc attgacgtat taggatatga ctttttgact
     5281 acgcatgttc cagaaaaagt aattattcga tttgataaag aagtagagta cgggaatgag
     5341 atcgaaagcc attttgaaca aatccctatc gaaaatggtg tcaaaacaaa acatgaaatc
     5401 cgaatgcaag aacagctgta ttttgaagca aatattatat ggaaaaataa ataaaaaaga
     5461 ggttgtgaca gaagggggca acttcaagaa ataagaagaa atttacgaaa attgtttctc
     5521 aaatttttgt aaattttcga cttagttttc gaagaagcta tttttgtcac agcctctctt
     5581 tttaagttta gctattagat aatgatgttt agttttggat aaacgaaatc aattcttcac
     5641 ccgacaaacc atagtttaca gcaatcaata gttttaaacg tgctttttga ctattaatac
     5701 tgttacagaa aataatcccc attttttcta gttcttttcc cccgccttca taatcataaa
     5761 caggctctgc gattccattg aaacaacgag aaacaagtac tacagggatt ttttggacaa
     5821 gaagcttttc caaagcagct aaggcttgtg gagggagatt ccccgcacct aaagcttcga
     5881 ttaccaaacc gtctactttc gtatgggcga tggcttccaa taagtcccct tgcatcccag
     5941 catatgcttt aacgatcggg atagtcccat ctacagcgga aatgtccagt cgctttgttt
     6001 ctaaaagttc ctgcaagaat aaaattcgat tcttagttac taagccgatg ggacccaatg
     6061 taggtgttcg gaaagtagca acatttgtgg tatgggtctt tgtcacgtag cgtgcagaat
     6121 ggatctcatc gttcatgacc accaaaaccc ccttatctag cgcttcttca caacttgcga
     6181 cacgaatcgc actttcaaaa ttataaaggc cgtcacttcc aagttcatta cttgaacgca
     6241 ttgccccagt caaaacaatt ggtaatttgt tgccaatcgt tgtatcaaga aaaaaagcag
     6301 tttcttctaa tgtatctgta ccgtgtgtga tcaccactcc ggatatacct gatgcataag
     6361 cggacaagat acgttctttt aataccaaca tgtgagaagg catgatgtgt ggtgagggaa
     6421 gattgaagat atcttcaact aataattcta catgcttggg gattgtgatt tctgcatcta
     6481 ataatgggtt tgccacatca ggactaacac cgcccgtgag catgttttct ttcatggcga
     6541 tcgttccgcc ggtgtgtagt acaagtattt ttttcatttt acagacttcc ttacgaataa
     6601 ttttgcatag ccgttaaatc ttattgtata ctatataaaa gagttttgct atcagtgaag
     6661 aataacgaat agcctaactt atcataaaaa agctgacctt attaaaatgg aaaagactca
     6721 accttgacct cctaatacaa tggagtatct ggttgagttt cttttacaag aaagcagcca
     6781 aggaaatcaa tcacgaaggt ggccggaaat gatcagaatc ggattaacat cgtttaaaga
     6841 acatgacaaa ttaactggta aaaagaataa cactctttat gaatatgcag ggtttctacc
     6901 cttagtggaa ttagatactg cctattatgg aattccccga aagtcctcag ttgagaattg
     6961 gataagtgaa gtacctgaat catttcgctt tgtagtcaaa gtctatcaag gaatcagcgg
     7021 tcaaggagac tggaagcctt attatcagac cgaagaagag atgattgcgc attttttaga
     7081 tgttatgtcg ccaatggtag atgctggaaa gatgttttgc tttttagtcc aattcccctc
     7141 atcattcaaa tgtacgaaag aaaatgtcca gtacttgcgc aaacttagaa agtggtttga
     7201 gggttatcct gtttctatag aattgcggga ttattcttgg tacagcgatg aatttcttga
     7261 acgtacaaga aactttatgt ctagagccgc tttttcttta gccatcatcg atgagccaca
     7321 gttgctgaat acaacgattc cttttgatcc atttgtcacc aatccgaact ttgctctttt
     7381 tcgattccat ggtaggaatg cgcaaggatg gcaagcaaat gataaagagt ggcgtaaaaa
     7441 aagaacgtta tatcgttatt cagaaaaaga actgcaagaa ctagcacatg aagtcaaagt
     7501 agttgattct aagacaaaag aggtaggtgt gatttttaat aataattctg gtggtgatgc
     7561 ggctgaaaat gcattagcac tgcaaaaaat attaaatatc aagccagatg gactcaatcc
     7621 aacccaaatg gatctgtttt aagggcttat tttattcaag aaaggggcga agtctatgat
     7681 caaagctatt ttttttgata ttgacggaac attggtcacc aatcgttcaa aagcgttgga
     7741 atcaacaaaa caagcaattg aatatgctcg gaaaaacggc attttatgcg gtgtagcaac
     7801 ggggcgcagc ccagtaaaga tcaaagaaat cattgatgaa ttggaattag atatgtatgt
     7861 cgtgtataat ggtcaactgg tttttactgc agatcgcacg attatcgacc acccttttga
     7921 acaaaaagta ttggaacata ttgttgaatt tgcagatgag aaccatcgac aaatcgtttt
     7981 tggtgcgaga aatcggttag atggatcaac gacgatgcta ttaggacaat cgatttttat
     8041 caagcgacta gtcagctttt taccgcgtaa attcccggta cgtctgatga aaaaaatcct
     8101 ccaggttttt agccctcacc gacaaaagga tcgttatgaa acgttagaga tattaaaaga
     8161 accaatttat caatgtatat tgctcagtcc ggagtcagaa cagaaaaagt tagaacatcg
     8221 ctttcccgag tgtacgtttc agcgttcaaa tacgtatact gtagatatca tcccaaaagg
     8281 tggttcaaaa ttactaggca tccaagcttt cgcaaatgct gtcgaaattg aaatggaaga
     8341 aattatggca ttcggcgatc attacaatga tatcgagatg ctgaaaggtg taggaattgg
     8401 agtggccatg gggaatgctc agatcgaagt aaaacatgct gcagactttg tgactcaatc
     8461 aaatgaagaa gatgggatat acgaaggact gaaacacttc ggtctgattt attagtcaat
     8521 gaaagaagga aaaaatcagt atggaagcag agacaccttt tcaaaaagta gaaacattcc
     8581 acaaaacttt tgatccaaga agaccagaga tacccacggc ttttacagaa aatgaagctc
     8641 atacacgtgc tgcgttcaaa atagaagaac ttgttgaatt tgttgcagct acttcaccta
     8701 ataaagaaca actgcatgaa gctgttatgc tgctgcacga agcattagat aaagcagaag
     8761 aaaaaatact tggtaaaaca cagtggggaa gtgatccgct ggttgaacaa gtagatgcac
     8821 tgacagatat cctttacttt acttatggtt ctttttcatt actaggaata gtgcctgatc
     8881 gaatattcga aatcgtccat caggcaaata tggggaaatt atttccagat ggaaaacctc
     8941 attatgaccc tgtaacaaat aaagtgttga aacctgaaaa ctggcagatg gaatatgctc
     9001 cagaacctaa aatcgcggca gaaatccaac gtcagattca agaagcgatt gctaagaaga
     9061 aatccgctga tataggcatg tgcacaaatg aaaaagagtg atcagagttt cactattttt
     9121 gttatcttta tacgatggaa aaacaaaaga ttttcgttta agttagtgaa tgcatgaaaa
     9181 tagtatgcta taatctagaa tagatgtctt ttgcacgaga gacgtattat ttggatttat
     9241 gccaagcaga acgccatctg ttgcataaaa gatgcatact gaaaacggag gaaccataaa
     9301 tgggaaataa tgttatcatc ggaatcgtca ttgcaatcat tatagtcgcc attgtcgtct
     9361 ttttggttag ttacttgatg aagaaaaaaa accaagatcg attggatgcc ttagaaaaac
     9421 gcaaggaaga attatttgac ttgccagtga ttgaagaagt cgatgatgtt aaaaaaatgc
     9481 atctggttgg gcaaagccaa aatacctttc gtgaatggag tcaaaaatgg aatgacatat
     9541 ctacttcctc gttcgctgaa ctagaaagtc aaattttcga agtggaaaac ctcaatgaaa
     9601 gttttcggtt cttaaaagga aaaaaagcag ttgttgaagc agaagctaca atggaccaaa
     9661 tggagaaaca agtgacggaa atccgtgaag gtctaaagga attgcgggaa acggaagaac
     9721 gcaactcact agctgttcag caggcacttg acgtctatga agaactgaaa gaaaaagtca
     9781 gagaagacga gcatgaatac ggaccagctc tttttgaatt gcaaaaacag atcaaagtga
     9841 tcgaaaaaga atttacgcag tttgtgactt taaacacttc tggagaccct gttgaagccc
     9901 gcgaggtttt ggataaagca gaaaaacata cgtatgaagt ggaagctctg atgaaaaaaa
     9961 ttccaccgct gtatgaggag ctccatacca ctttccctga acaacttgaa gaaatcagtg
    10021 atacttacga gaaattgatg gaagaacaat atgtattccc agaagaagat cttgctgaag
    10081 atatcgcaaa agtaagtcga agaattgaaa attcattagc caatcttgaa aaaacagaag
    10141 ttgaaacggt cgaatttgaa aaccgtgaaa cagctgattt gattgattcg ttatatgata
    10201 tcttagaacg ggaaatggaa gctcagagat atgtaaaaac taaccaatca acgattgcag
    10261 agtatatcaa acatacaaca aagaataacc gtcaattatt gattgagctg gatcacacat
    10321 ctcaaagcta tacattgaac cacaatgaaa ttggccgtgt acgaggtttc caaaccgaag
    10381 tggaagaaat ggaacgtcaa aatgaacaga tgattccaca gatccgtcaa cacgaaattc
    10441 cttattcaga agtgcgcacg ttctataaaa cagtcttcaa agtcttagaa gatatcgaaa
    10501 agcaacaagt agagatcgac gactcattac atgaattgag aaaaggcgaa aaagaagcgc
    10561 aagaaaagat cgatacattc gaattcaaat tgcgcagttt gaaacgtttt gtagaaaaac
    10621 aacgtttgcc tggcttgccg aatgattacc tagaattctt ttttgttgct actgaccgta
    10681 ttgaagaact aagtgttgtc ttgaataaaa ttcgagtaaa catggaagag gtcaatcgtt
    10741 tagttgctct atgtgaagaa gatctagaat tattagataa gaagacacat gatttagtag
    10801 atgcagcagc attaacggag caaatgctcc aatatgcaaa tcgttatcgt catacacacg
    10861 aagaagtgag agaagcaatt gataaaagct actatttatt caataaagaa tatcactatc
    10921 aagaagcatt agacgaaatc ggtacagcat tagagcgagt agaacctggt gctttcaaac
    10981 gcatcgaaga tttttatttt aatcatccag acttagtata aagcaaaaaa gttagtaaac
    11041 cagcaagagc aagacggatt attgtcttgt tcttgtcttt ttttgtatat ttgaaaattg
    11101 aaacaagtga aaatttcacc tgctcttgaa agcaccttgg acaaccgtta taatagaaaa
    11161 gaatttcaaa aaagaggggt ttcttatgat atattttgat aacagtgcaa caaccccaat
    11221 ctttccgcaa agcttagatg catatgtaaa aaccagtcag cggattattg ggaatccatc
    11281 aagcttgcat gatttaggca atcaagctaa ccggttgttg cagcaagcaa gaaagcagat
    11341 cgctgatttg atccatgtag aaccagaaga aatctatttt acttcaggcg gaacagaagg
    11401 agataactgg gtattaaaag gaacgatgat cgaaaaaaga gaatatggga atcatatgat
    11461 catttcagga gtcgaacatc cggccgtcag cgagacagca gaacaattaa aagaattagg
    11521 aatcgaagta agtatcgccc cagtggataa acgcggattt gtacgtgtcg acgaattgaa
    11581 agaattaata agaaaagaga cagtattagt ctctgttatg gctgtgaata atgaagtagg
    11641 atcgattcag cccatccagg aaatcagtga tcttttggat gattttccta aaattcattt
    11701 ccatgtagat gctgttcagg cgattggaaa agtaccattc tcgcaatggt tgacagaaag
    11761 agttgatttt gcaacatttt cagctcataa attccatggc ccacggggaa ctggtttcat
    11821 ttattggaaa aagggtcgtc gtttagcacc attactgaca ggtggagggc aagaaagaaa
    11881 tcagcgaagt gggacagaaa atgttccagg tattgtggct atggttaaag ctttacgttt
    11941 atcattagag ataaaggaac aaaaacctga gcagacacaa cgcttaaaag tagctctaat
    12001 caatgcgttg aagacgtatg agaaagtgac gattttttca gaaggaactg actatgcacc
    12061 gcatatttta tgttttgctc taaaaggaat tcgcggcgaa gttctcgtcc atgccttaga
    12121 agaaaaacaa atatttgttt ctactactag tgcctgctcc tcacgtaaac atatggcaag
    12181 cagtacattg cacgcaatgc atgtaccaaa tgatctggct acatctgctg ttcgaatcag
    12241 cttagatgaa aacaattcgc ttgcagaagt cgagcaattt atgattattt tcaagcagct
    12301 ttataagaaa ttttctgcaa tcaattaaaa gaggagtgtt cttgtgaaat acagtgaaat
    12361 tatggtccgt tatggggaac tttcgacaaa aggaaagaat cgaaaaagct ttatcatgca
    12421 actagcacaa aatgtcagac aagctttgaa agactttcca gaaatcaaaa tccatgctga
    12481 tcgggaccgg atgcatcttt tattgaatgg cgcagacggg cagctagtta ttccaaaact
    12541 agcaaaaata tttgggatcc aaaatttctc tccaagtatt cgtgtagaaa aagatgtcga
    12601 agttttgaaa caagctgttc aagacatcat gaaagaaatt tatactggga aagaaacatt
    12661 taaaattatc gctaaaagaa gcgatcacca atttgaattg acaagtaatg aattgaatca
    12721 aacattaggt aatgctgttt ttgatatttt cccgcatatc caagttcaaa tgcgtcaacc
    12781 agatattcca ttacgtgtag agattcggcg tgatggcgct tatctatcgt atgaaacgat
    12841 caagggagct ggaggattgc ccgtaggaac aagcggaaaa ggaatgctta tgctttctgg
    12901 ggggattgat tctcctgtag cagggtactt tgccatgaag cggggagtgg aaattgaagc
    12961 tgttcatttt gctagtccgc cgtatacaag tgaacaagca ctgcaaaaag caaaagatct
    13021 aacagccaaa ctagcacctt atgtgggaac gatccatttt atcgaagtgc catttaccga
    13081 gattcaagaa gaaatcaaaa agtcagtgcc acaaggatat tggatgacaa ttactcgtcg
    13141 aatgatgttg cgtttgaccg atgaaattcg ccgtattcgt catggtttag tcattttgaa
    13201 tggtgaatca ttaggtcaag tggcttcaca aactttgcaa agtatggtgg ctatcaatga
    13261 agtaacgaat acgccaatca ttcggcctgt agcgacaatg gataaacttg aaatcattga
    13321 agtagcagaa cagatcgata catttaatct ttctatccag ccttttgaag attgctgtac
    13381 gatttttgct cctccacagc caaaaacacg ccctcaactg gaaaaggtcc atcaatatga
    13441 agagcgttta gcaatagatg aaatgatcca acgagcacta gctggcttga agatggaaga
    13501 gatacaagca gaatcgtcta atgaaaaaac tgaagaattt tcagaattgc tataagaggt
    13561 tatgaaaata gtgttttgac cagagtatta gagaaacaaa acgaaaaaat agctccatgt
    13621 attttaccgt ttttgaacta atgtcgaaga ttagctgatt gaataccatt tatcaagaag
    13681 ttatggaaaa gccggttctt ttttactaac tgaaaaataa aattttattt agagatcaag
    13741 ctacagttga taaatgatat aaaaaagaga atttcccatg aaatagcgag gttgtgacag
    13801 aagtgggcag cttcaagaaa taagaaggaa tttacgaaaa ttgcttttca aatttttgtg
    13861 aaatttcggc ttatttttcg aagaagttac ttctggaaca ccgtgtatca gaggttg
//
LOCUS       DACGLE010000069        13807 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0534
            SAMEA3304449-rid9560453.denovo.073, whole genome shotgun sequence.
ACCESSION   DACGLE010000069 DACGLE010000000
VERSION     DACGLE010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304449
            Sequence Read Archive: ERR985064
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13807)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13807)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702225.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702225.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 20:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,045
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 27 of 67
            Pseudo Genes (incomplete)         :: 25 of 67
            Pseudo Genes (internal stop)      :: 19 of 67
            Pseudo Genes (multiple problems)  :: 4 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13807
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304449-rid9560453.denovo.073"
                     /strain="VREN0534"
                     /db_xref="taxon:1352"
     gene            complement(76..2073)
                     /gene="tkt"
                     /locus_tag="IXT20_002186"
     CDS             complement(76..2073)
                     /gene="tkt"
                     /locus_tag="IXT20_002186"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HAQ4860618.1"
                     /translation="MFDKIDQLGVNTIRTLSIEAVQKANSGHPGLPMGAAPMAYALWT
                     KHLKVNPTTSKNWADRDRFVLSAGHGSAMLYSLLHLAGYQVTIDDLKQFRQWDSKTPG
                     HPEVHHTDGVEATTGPLGQGIAMAVGMAMAEAHLAATYNKENFNVMDHYTYAICGDGD
                     LMEGVSQEASSMAGHMKLGKLIVLYDSNDISLDGPTSKAFTENVGARYEAYGWQHILV
                     KDGNDLEAISKAIEEAKAETDKPTLIEVKTVIGYGAPKEGTSAVHGAPLGEDGIKMAK
                     EVYGWEYPDFTVPEEVAARFHQTMIEEGQKAEDAWNEMFANYKKAYPELAQQFEDAFD
                     GKLPENWDAELPTYEVGSSQASRVSSKEVIQELSKAIPSFWGGSADLSGSNNTMVAAD
                     KDFTPEHYEGRNIWFGVREFAMASAMNGIQLHGGTRIYGGTFFVFVDYLRPAVRLAAI
                     QHTPVVYVLTHDSVAVGEDGPTHEPIEQLASVRCMPGVQVIRPADGNETRAAWKVAME
                     TTDAPTILVLSRQNLPVLPSTKEVADEMVKKGAYVLSPSQGETPEGILIATGSEVDLA
                     VKAQKELAEKGKDVSVVSMPSFDLFEKQSSEYKESVLPKSVKKRVAIEAAASFGWERY
                     VGTEGATITIDHFGASAPGTKILEEFGFTVENVVNTYNQLS"
     gene            complement(2175..2426)
                     /locus_tag="IXT20_002187"
     CDS             complement(2175..2426)
                     /locus_tag="IXT20_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 family protein"
                     /protein_id="HAQ4860619.1"
                     /translation="MLSPEKIERINELARKKKENGLSKAEEQEQINLRKEYLEAFRSG
                     MRHHIEGMKVVDPEGNDVTPEKLKEIQKKKGLHNRNDNF"
     gene            2573..3196
                     /gene="lexA"
                     /locus_tag="IXT20_002188"
     CDS             2573..3196
                     /gene="lexA"
                     /locus_tag="IXT20_002188"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HAQ4860620.1"
                     /translation="MARQTESRQIEVLRYIHEQVTQKGYPPTVREIGEAVQLSSTSTV
                     HGHLARLEKKGFIQRDPTKPRAIELTKQGLEKIGIRPTTIPVLGVVTAGEPILAVEDA
                     SDFFPIPPNLSSEEGSLFMLTIRGDSMINAGILDGDNVIVRKQETAQNGDIVIAMTAE
                     DEATCKRFYKEKNFFRLQPENDLLEPIILDQVSILGRVVGLYRSHIY"
     gene            3376..3594
                     /locus_tag="IXT20_002189"
     CDS             3376..3594
                     /locus_tag="IXT20_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4860621.1"
                     /translation="MKSMRRLGLVLTVLFLGIIIGNFGIRPALLVFVPLFTLWFMLWD
                     EKKYRQSERRRIHEDHFYHEPYYRSRQS"
     gene            complement(3883..4395)
                     /locus_tag="IXT20_002190"
     CDS             complement(3883..4395)
                     /locus_tag="IXT20_002190"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HAQ4860622.1"
                     /translation="MDLKDYIASIPDYPSKGIVFRDISPLMADGDAYREATKQIVNYA
                     KEKRIDMVVGPEARGFIVGCPVAYELGVGFAPVRKKGKLPRETIEVTYGLEYGTDTLT
                     LHKDAIKPGQRVLICDDLLATGGTIKATIELIEQLGGVVVGCAFLIELMDLHGRDKIK
                     GYDIITLMEY"
     gene            complement(4409..6706)
                     /gene="recJ"
                     /locus_tag="IXT20_002191"
     CDS             complement(4409..6706)
                     /gene="recJ"
                     /locus_tag="IXT20_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HAQ4860623.1"
                     /translation="MRQANFDWKLSETEPSESFLQMIRELKLSPFVGKLLWQRGYHEE
                     KDINRFLHPKEQELHDPYLIHDMDKAISRIQEAVISGEKILVYGDYDADGITSATVMK
                     ETLELLGAEVEVFLPNRFEHGYGPNQSVYQEKIEEGIQLIITVDNGVAGNDAVAYAQQ
                     AGVDVIITDHHELPENLPDAYAIVHPRHPAGHYPFPDLAGVGVAFKVASALLEEPPAE
                     FLDLVAIGTIADLVSLTDENRILVSLGIDAIHHSERIGLQALFEESGVKMRDADETTI
                     GFSIAPRLNAIGRMGDPRPAVSLLATFDEEEASVQAKKLNEINEERKAIVEQITQEAL
                     AMVNKENHIHLLVNRGWHEGVLGIVAGKIMNETGKPTLVLTLKEDGSAKGSGRSIEAL
                     NLFEMLDQMRDLFTYFGGHHAAVGLTMPSENVTILQEKMNQYIVDHQIDLMRGPELRI
                     DEVLLPNEVTVERIDELKLLAPFGTDNPLPQFLFRQVQAENIKRIGANQQHLKFVLSD
                     ASSQLDAVAFGFGTQEEELLNNQVDVVGKLSINEWNGRKKPQLMVSDFSVGGLQVFDW
                     RAKRFREQSRTSEGSLYLAFDSSTLKFVPEIIQEAAIVFEGLDSVKAIIEKRKPNSLV
                     VMDCPDDLADLKEVFRSYSFNRVYLMGISPDEAYLNGVGTREQYAKLFKLIHSQERID
                     IRHKIKAIAQYLRIPEKLLIFMIQVFFELKFVTIENGVLQKVASPESHPLTESRLYQR
                     RLNKIKVEEFLLLSDIPTIKKWLTT"
     gene            complement(6848..7282)
                     /locus_tag="IXT20_002192"
     CDS             complement(6848..7282)
                     /locus_tag="IXT20_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1049 domain-containing protein"
                     /protein_id="HAQ4860624.1"
                     /translation="MKKQSNVILGFVLVLIIVLFAVLNNKNVPVSFGFTSFSAPLILV
                     IIGSALIGALIVFLTASATLWQQKKQIKQLTQEMSEYQTELQKKIEEAKEEQQREFRN
                     ERAELEAAYQAELQAKTAQISQLKAPEEQKTQSPKQSFNYFD"
     gene            complement(7292..8080)
                     /locus_tag="IXT20_002193"
     CDS             complement(7292..8080)
                     /locus_tag="IXT20_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAQ4860625.1"
                     /translation="MNLEDKVVLVTGSSGGLGAQICYEAAKQGAIVISCARRMAFVEG
                     VRDECRRLSGKEAYAFKVDVSNPESVDELYEKVMEEVGRVDILVNNAGFGIFEDFLTF
                     DLGKAYDMFEVNILGMMVLTQKFAIDMAERRQGHIINIASMAGKMATAKSTVYSATKF
                     AVLGFSNALRLELKPLGVAVTTVNPGPIETNFFDKADPSGSYLEKVGQIVLEPAKLAK
                     MIVRNMRHPKREINQPFILEVASKFYTLFPTIGDYLASGIFNKK"
     gene            complement(8077..9018)
                     /locus_tag="IXT20_002194"
     CDS             complement(8077..9018)
                     /locus_tag="IXT20_002194"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HAQ4860626.1"
                     /translation="MELQFLGTGAGVPAKHRNVTSIALKLLDERNEVWLFDCGEGTQM
                     QILRTTIRPRKIGKIFITHLHGDHIFGLPGLISSRSFQGGDTPLEIYGPKGIEEYIKV
                     SLGISQTRLSYPLKFIELNETAPIFTDQQFSVYTKKLNHGIDSFGYRVVEHDHKGELQ
                     VDRLKELGIPAGPLYGKLKQGETIQLEDGRTINGKDFVGPDKKGRIVTILGDTRKTHN
                     SVVLAENSDILVHESTFNKDEARMAHNYFHSTTHQAAEVAKEAQAKRLLLTHISARYL
                     GKAALELEKEAQEVFENTTIMKDFDSIEIPFREEEEA"
     gene            complement(9195..9782)
                     /locus_tag="IXT20_002195"
     CDS             complement(9195..9782)
                     /locus_tag="IXT20_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1269 domain-containing protein"
                     /protein_id="HAQ4860627.1"
                     /translation="MSKRVIILNFQVESQAYQAFSEIKKLHAVRSIKGEQMAVVSHDP
                     NGTHQFKIEDFIDFTGNNKSSTGGLIGMLVGVLGGPLGILLGWFAGGMIGATQDAKEI
                     KDAQTVFDFVGNKIGEGATGLILIADEEDNRPLNQLVMMELGGEITRLDYEDVEKDVH
                     DAKEAENHAKEAAQKTWKDKHPQTSNDNESSGSSN"
     gene            complement(10003..11316)
                     /gene="obgE"
                     /locus_tag="IXT20_002196"
     CDS             complement(10003..11316)
                     /gene="obgE"
                     /locus_tag="IXT20_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003126661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HAQ4860628.1"
                     /translation="MSMFLDQVTIDVKAGKGGDGMVAFRREKYVPDGGPAGGDGGRGG
                     DVILIVDEGLRTLMDFRFNRHFKAQPGENGMSKGMHGRGSEDTYVKVPQGTTVRDAET
                     GALLGDLIENGQTLVVAKGGRGGRGNIRFASPRNPAPEIAENGEPGQERKIELELKVL
                     ADVGLVGFPSVGKSTLLSVISSARPKIGAYHFTTLVPNLGMVTTSDGRSFAAADLPGL
                     IEGASQGVGLGTQFLRHIERTRVILHVIDMSGMEGRDPYEDYLAINKELSTYNLRLLE
                     RPQIIVANKMDMPDAPENLVKFKEQLNKEKEDEFADDIPVFPISGVTRQGLDALLNAT
                     ADLLEVTPEFPLYEEELEEETVHYGFNPEGPEFQIDRDSDATWILSGEKIEKLFQMTN
                     FDHDETVMRFARQLRGMGVDEALRARGAKDGDLVRIGEFEFEFVE"
     gene            11604..12614
                     /gene="gap"
                     /locus_tag="IXT20_002197"
     CDS             11604..12614
                     /gene="gap"
                     /locus_tag="IXT20_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HAQ4860629.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIKEVSDDIEVVAINDLTSPTMLAQ
                     LLQFDSTHGTYPGTVTATEDSIVVDGEATRVYAEPDASKIPWAKENGVDIVLECTGFY
                     TSEEKAKAHLDAGVKRVVISAPAGAMKTIVYNVNDDTLDENDKIISAGSCTTNCLAPM
                     AYFLNNEFGIEVGTMTTVHAYTSTQMLLDGPVKGGNLRAARSAADNTIPHSTGAAKAI
                     GLVIPELQGKLQGHAQRVPVVDGSLTELVSVLKTKVTADQVNEAIKKHTVDNPSFGYD
                     DRQIVSSDVIGTTQGSIFDPTQTEVTTAGDFQLVKTVAWYDNEYGFTCQMIRLLEKFA
                     NL"
     gene            complement(12699..13634)
                     /locus_tag="IXT20_002198"
     CDS             complement(12699..13634)
                     /locus_tag="IXT20_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAQ4860630.1"
                     /translation="MKLWMKILLGVALALIVALVFAGNYFYNYAVVPSEKDFLDEDTP
                     GTTQTSKETDAHKWFSSKENRSDWTITSKDGLKLSAIYLPAETKSEKTALVAHGYMGN
                     AETMTNYAKMFHDMGYNVLVPDARGHGKSEGDYIGFGWPERKDYVQWINKVLENNGKS
                     QEIVLYGVSMGAATVMMTSGEKLPDNVKAIIEDCGYSSVHDELAYQLDDMFSLPAFPL
                     MQVTSLVTKVRAGYFFGEANAVEQLKKNQRPMLFIHGDEDTFVPFEMLDKVYRATKGP
                     KEKYVVKGAEHAEAYKTDPEKYQQVVQQFLSQYVH"
BASE COUNT         3811 a         3094 c         2439 g         4463 t
ORIGIN      
        1 ttcttctaac acataaagaa cagctgtctc caacttgcag acagctgttc ttatcagtca
       61 aacaattgct tggatctatg atagttgatt gtacgtattc acgacatttt ctactgtaaa
      121 tccaaattct tctaaaattt tagtgccagg ggcagatgca ccgaaatgat cgattgtaat
      181 cgttgcgccc tctgttccta cgtagcgttc ccatccaaat gatgcagcag cttcgatagc
      241 cacacgtttc ttcactgatt taggaagtac actttcttta tattcagaag attgtttttc
      301 aaagaggtca aagcttggca tagaaacaac agaaacatct ttgccttttt cagccaattc
      361 tttttgagct ttcacagcca aatcaacttc agaacctgtt gcaatcaaaa taccttctgg
      421 tgtttctcct tgtgatggag aaagaacata cgcgcctttt ttcaccattt catctgctac
      481 ttcttttgta gaaggcaata ctggaaggtt ttgacgactc aatacaagga tagtaggcgc
      541 atctgttgtc tccatcgcta ctttccaagc agcacgtgtt tcatttccgt ctgccggacg
      601 aatcacttga acaccaggca tgcagcgaac agatgctagt tgttcaatcg gttcgtgcgt
      661 tggtccgtct tctccgacag caaccgagtc atgtgttaag acgtaaacaa caggagtatg
      721 ttggattgct gctaaacgaa ctgctggtct tagataatcg acaaagacaa agaatgttcc
      781 accgtagata cgagttccgc catgaagttg gataccattc atagccgaag ccattgcaaa
      841 ttcgcggaca ccgaaccaga tgttgcgtcc ttcataatgt tcaggagtga aatctttatc
      901 tgcagccacc attgtattat tggaacctga caagtccgct gaaccacccc agaagcttgg
      961 tatagctttc gataattctt ggatgacttc tttacttgaa acacggcttg cttgactgct
     1021 tccaacttca taagttggca attctgcatc ccagttttct ggtagtttgc catcaaaagc
     1081 atcttcaaat tgttgtgcta attcaggata agctttttta taattggcaa acatttcatt
     1141 ccatgcatct tcagcttttt gtccttcttc tatcatcgtt tgatggaagc gggcagctac
     1201 ttcttctgga acagtgaaat ctggatactc ccaaccataa acttctttag ccatcttgat
     1261 gccatcttca ccaagaggag caccatgaac agcagatgtt ccttctttag gagcaccata
     1321 gccgatgact gtttttactt cgatcaatgt tggtttgtct gtttcagctt ttgcttcttc
     1381 aatcgcttta gagattgctt ctaggtcatt tccgtctttc actaagatat gttgccagcc
     1441 atatgcttca tagcgagcac caacgttttc tgtgaaagct tttgaagttg gaccgtctag
     1501 tgaaatatcg tttgaatcgt ataaaacgat caatttccct agtttcatat gacctgccat
     1561 cgaacttgct tcttgagata ccccttccat caagtctccg tcaccgcata ttgcatacgt
     1621 atagtgatcc atcacattaa agttttcttt gttatacgtt gctgcaaggt gcgcttctgc
     1681 cattgccata ccgacagcca ttgcaatccc ttgtcctaat ggccctgtag tggcttctac
     1741 accatctgta tgatgtactt caggatgtcc tggtgtttta gaatcccatt gacggaattg
     1801 tttcaaatca tcgattgtca cttgatagcc agctaaatga agcaagctat acaacattgc
     1861 tgacccatgt cctgccgaaa gaacgaaacg gtcgcggtca gcccagtttt ttgatgtcgt
     1921 tggatttact ttcaggtgtt ttgtccataa cgcatatgcc ataggtgctg cgcccatcgg
     1981 caatccagga tgtcctgagt ttgctttttg gactgcttca atacttaatg tacgaattgt
     2041 gttaacgcca agttggtcaa ttttgtcaaa caaaataacc cctcctatta atttaggttt
     2101 tttttacctt tatttcttac actttcattc tataagatgt cacacgaaaa agcaatcttt
     2161 ttcagtattt tttactaaaa gttgtcattt cggttgtgta agcctttttt cttttggatt
     2221 tcttttaatt tttccggggt cacgtcattg ccctcaggat cgacgacttt cattccttcg
     2281 atatgatgac gcataccgga acgaaaagct tccaaatact ctttgcgcaa gttgatttgt
     2341 tcttgttctt cggcttttga gagaccattt tccttctttt tacgtgcaag ttcattgatc
     2401 cgctctattt tttcaggtga cagcatgctc agccctcctt ttactctcct tcatattata
     2461 taaaaactga aaaaaaaaca actagataaa ctaaataaaa tacgaacatt cgtttgattt
     2521 agcgcttttt atatggtact atttatttat caagtgaaag aaggtgtacc ttgtggcccg
     2581 acaaactgaa tcaagacaaa tcgaagtctt acgatatatc catgaacaag taacccaaaa
     2641 aggatacccg ccgactgtcc gtgaaatcgg ggaagctgtt cagctttctt ctacttctac
     2701 tgttcacggt catcttgcac ggcttgaaaa aaaagggttt atccaaagag atccgacgaa
     2761 acctcgtgca atcgagttga caaaacaagg tttagaaaag attgggattc gtccaacaac
     2821 tattccagta cttggcgtcg taacagctgg agaacccatt ttagcagtag aggatgcttc
     2881 agacttcttt ccaatccctc ctaatctttc ttccgaagaa ggcagtctct ttatgctgac
     2941 aattcgtgga gatagtatga tcaatgctgg gatattggat ggtgacaacg tgatcgttcg
     3001 gaagcaagaa actgctcaaa atggcgatat tgtcattgca atgactgccg aggacgaagc
     3061 gacttgcaaa cgattctata aagaaaaaaa ctttttccgt ctgcagcctg aaaacgactt
     3121 gctcgaacca atcatcttag atcaagtatc tatattagga cgagtagtag gactttatcg
     3181 tagtcatatc tattaattgc tactaattaa aagaaaactt tttaaaaaga gtttgagacg
     3241 ttggtctcaa actctttttt cgcccgggtt cttcttcttt caaattttta tcacttccct
     3301 ctttacaaac cgaacaaata ttcgtatact aaatatacga acaattgttc gcataaatta
     3361 aagaggtgag aattcatgaa atcaatgaga cgtcttggct tagtattaac tgttttgttt
     3421 ttaggtatta ttattggtaa tttcggcata cgtccagctt tacttgtgtt tgttccgtta
     3481 tttactttgt ggttcatgtt gtgggatgaa aagaagtacc gtcaatctga gcggcgcaga
     3541 attcatgaag atcatttcta tcatgagccc tactacagat ccaggcaatc ataataagga
     3601 ttgactgcta attattcaaa agacaagaag ttaaactttt acgggggtgt gacaaaaatc
     3661 ggtcacatcc tttttttcga ataaacggtg ttctaaagcc agtttttcgg tattaagtca
     3721 aattgcggaa aaagtgattt tttaaacttc tactggtctt tgcttgtttc catttataaa
     3781 aaaatagcga ccctcaaaag tcagatagag aatctgactt ttgagggtcg ctttaaaatc
     3841 gcttctttaa gaataacatc aagaatagct tcttttttct gcttaatatt ccattaatgt
     3901 aatgatgtca tatcctttga ttttgtcgcg tccatggaga tccatcaatt cgatcaaaaa
     3961 tgcgcagcct acgactacac cgcctaattg ttcgatcagt tcgatcgtcg ctttgattgt
     4021 tcctccagta gctagcaaat cgtcacaaat cagcacacgt tgacctggtt taatggcatc
     4081 cttatgcaat gtcaatgtat ctgttccata ttctaatcca taagtcactt cgatggtttc
     4141 tcgaggtaac ttcccttttt tgcggactgg ggcaaaaccg actcctaact cataagcaac
     4201 aggacatcct acgatgaatc cacgggcttc tggcccaact accatatcaa ttcgtttttc
     4261 ttttgcataa tttacgattt gtttagtagc ttcccgataa gcgtcaccat ctgccatcag
     4321 tggggaaata tctcgaaaca caattccttt tgacggatag tcgggaatac tggcaatata
     4381 atcttttaaa tccactcttt gtttcctcct aggttgttaa ccatttcttt atggtaggaa
     4441 tatcgctcaa aagcaaaaat tcctcaactt tgattttatt gagccgtcgc tggtataatc
     4501 tgctctcagt caaaggatga ctttctgggc tagcgacttt ttgtaaaaca ccgttctcta
     4561 ttgtaacaaa tttcaattca aaaaacacct gtatcataaa aattaataat ttttcaggga
     4621 tacgcaaata ttgagctatt gcttttattt tatgacgtat atcaattcgt tcttgagaat
     4681 ggatcaattt aaacaatttt gcgtactgtt ctcttgtgcc taccccattc aaataggcct
     4741 catcaggtga aatccccatc agataaacac gattgaatga ataagaccga aaaacttctt
     4801 tcagatccgc taaatcatct ggacaatcca taacaactag agagtttggt tttctttttt
     4861 caattattgc ctttacagaa tctagtcctt cgaaaacaat cgcagcttcc tggataatct
     4921 caggcacgaa tttcaacgtc gaagaatcaa atgctaaata aaggctacct tccgacgttc
     4981 ggctctgttc acggaaacgt tttgcccgcc aatcaaatac ttgcagacca cccacagaaa
     5041 aatcagatac catcagctgc ggtttcttac gcccgttcca ttcattgatc gaaagctttc
     5101 ctaccacatc tacctgatta ttcagcagtt cttcttcttg agtaccaaaa ccaaaagcga
     5161 ccgcatcaag ctgactcgat gcatcggaaa ggacaaattt cagatgctgt tggtttgctc
     5221 cgattctttt gatattttct gcttggactt gtcgaaataa aaactgaggc aaaggattat
     5281 ccgtaccaaa tggcgccaat agtttcagct catcaattcg ctctactgtt acttcatttg
     5341 gcagcaatac ttcatcaatt ctcaactcag gccctctcat caagtcaatt tgatgatcga
     5401 ctatatattg gttcatcttc tcctgtagta tagtgacatt ttcacttggc atcgttaaac
     5461 cgactgctgc atgatgtcct ccgaagtaag taaataaatc tcgcatctgg tcaagcattt
     5521 caaaaagatt caaggcttca atactacgtc cagatccttt agctgatcca tcttctttta
     5581 aagtaaggac aagcgttggt ttacccgttt cattcattat tttcccagca acaatgccca
     5641 aaacgccttc atgccatcct cggtttacca atagatggat atgattctcc ttattgacca
     5701 tcgctaatgc ttcttgtgtt atctgctcta cgattgcttt tctttcttca ttgatctcat
     5761 tcaatttttt tgcttgaacg gatgcttcct cttcatcaaa cgtcgcaagt aacgagacgg
     5821 caggacgtgg atcgcccatc cgaccgattg catttagtct gggagcaatc gaaaaaccga
     5881 tcgttgtttc gtctgcgtcc ctcattttta cgccgctttc ttcaaatagc gcctgaagtc
     5941 ctatccgttc tgaatgatga atggcatcga ttcctaatga aacaaggatt cgattctcat
     6001 cagtcaatga aactaaatcg gcgatcgttc ctatcgctac taaatccaaa aattctgcag
     6061 gcggttcttc caataatgca gaagctactt tgaatgctac tccgactcct gccaaatctg
     6121 gaaatggata gtgccccgct ggatgacgag ggtgaacaat cgcataagcg tctggtaaat
     6181 tctcaggtag ttcgtgatga tctgtaatga tcacgtctac accagcttgc tgagcgtatg
     6241 ctaccgcatc atttcctgct accccgttat caacagtgat gatcaactgg attccttctt
     6301 cgatcttttc ttggtatacc gattggtttg gtccataccc atgctcaaat cgatttggca
     6361 ggaatacttc tacttctgca ccaagtaatt ccagtgtttc tttcattacc gttgcactgg
     6421 tgatcccatc tgcgtcataa tcaccgtaaa cgaggatttt ttctccactg attacagctt
     6481 cttggattcg gctgattgct ttatccatat cgtgtatcag atacggatca tgtaattctt
     6541 gttcctttgg atgtaaaaat cggttgatat ccttttcctc atggtaacct ctttgccaca
     6601 gtaacttacc gacaaatgga gataatttca gttcacggat catttggaga aaggattcag
     6661 aaggctctgt ctcgcttaac ttccaatcaa aatttgcttg tctcactttc tcactcctaa
     6721 cttagttatt atagcaaagg aaatcagcaa aaaaaagcag aaagaaagaa taaaaacaga
     6781 tcagatccgc tagacagatg ttcagcagga cctgatcttt tgtacgacca tttatgaaat
     6841 atccctatta gtcaaaataa ttgaagcttt gtttgggcga ttgagttttt tgttcttccg
     6901 gcgctttcaa ttgactgatt tgtgcggttt tcgcttgtaa ctccgcctga taagcagctt
     6961 ccaactccgc acgttcgttt ctaaactcgc gttgctgttc ttcttttgct tcttcaattt
     7021 tcttttgaag ttctgtttga tattctgaca tttcctgagt cagctgtttg atttgctttt
     7081 tttgctgcca tagtgttgcc gaagcagtca aaaatacaat cagtgcacca atcagcgcag
     7141 aaccgatgat cactaagatc aatggcgcag aaaaggaagt gaagccaaaa cttacaggaa
     7201 catttttatt gttcaataca gcaaaaagaa caataatcaa aactaaaaca aatccaagaa
     7261 tcacattgct ttgctttttc aattccttca cctacttttt gttaaaaatt ccgctggcta
     7321 aataatcacc aatcgttgga aacagcgtat aaaattttga tgcaacttct aaaataaacg
     7381 gttgattgat ttcccgtttt ggatgtctca tattgcggac gatcattttt gccaacttgg
     7441 caggttctaa aacgatttgt ccgacttttt ccaaatagct tccgcttgga tcagccttat
     7501 cgaagaaatt tgtctcgatt ggacctggat tcactgtcgt aacagctacg ccaagtggtt
     7561 tcaattctaa tcgcaaagcg ttggagaagc caaggactgc aaatttcgtg gcagaataaa
     7621 ccgttgattt tgcagttgcc atttttccag ccatagaggc gatattaatg atatgacctt
     7681 gtcttctttc tgccatatcg atcgcaaatt tttgtgtcaa aaccatcatt cctaagatgt
     7741 tgacttcaaa catatcgtat gcttttccaa ggtcgaatgt aaggaagtct tcgaaaatcc
     7801 caaaacccgc attgtttacc agaatatcga cgcgcccgac ttcttccatc accttttcat
     7861 ataattcgtc cacgctttca ggattgctga cgtctacctt gaaagcataa gcctctttgc
     7921 cgctcaagcg tctgcactcg tctctgacac cttctacaaa tgccattctt cgtgcgcaac
     7981 ttataacgat tgctccttgc tttgctgctt cataacagat ttgtgcgccc agaccgcctg
     8041 aactacctgt tactaaaaca actttatctt ctaaattcat gcctcttcct cctcgcgaaa
     8101 tggaatctca atgctatcaa aatctttcat aatcgtcgtg ttttcaaaca cttcttgtgc
     8161 ttctttttca agctctaaag ctgcttttcc taaatatctc gcactgatat gagtaagtag
     8221 cagccgtttt gcctgcgctt cttttgcaac ttcagcagct tgatgagtcg tggaatggaa
     8281 atagttgtgc gccattcttg cttcatcttt attaaatgtg ctttcatgta ccagaatatc
     8341 gctattttcc gcaagcacaa cggaattatg tgtttttctc gtatcgccta aaatagtcac
     8401 gattcgtcct tttttatctg gacctacgaa atcttttcca ttgatcgtcc gcccatcttc
     8461 tagttggatc gtttcacctt gcttcagttt gccgtacagt ggtccagctg gaatacctaa
     8521 ttcttttaga cgatccacct gcaattctcc tttatgatca tgttcaacca cacgataacc
     8581 aaaactgtcg atcccatgat ttagtttttt tgtataaaca gaaaattgct gatcagtaaa
     8641 aatcggggct gtttcattta gctcaatgaa tttcagcgga taacttaaac gcgtttgcga
     8701 aatacctaaa gagactttta tgtattcctc tatccctttt ggcccgtaaa tctctaatgg
     8761 cgtatcccca ccttgaaatg accggctgct gatgagcccg ggaagtccaa aaatatgatc
     8821 gccgtgtaaa tgtgtaatga aaattttacc gatttttctc ggacgaatag tcgttcgcaa
     8881 aatctgcatt tgcgtgcctt cgccacagtc gaacaaccaa acttcattgc gctcgtctaa
     8941 aagtttcaat gctatacttg tcacatttct atgttttgca ggaacacctg cacctgttcc
     9001 taaaaattgt aattccataa tctacctgac tttctttaag ttcctcttta attttagggt
     9061 aaaaagaagg gataatcaac cgtaagtcga gaatgggttc aaattaaaaa taacgttcta
     9121 aagcgatagc gttttctttg aaaattacta taaaaacagc agcgaacccg tttgagatcc
     9181 tctgctgttt tttattaatt tgaagatcca gaactttcat tgtcgttgga agtttgcgga
     9241 tgtttgtcct tccaagtctt ttgtgcagct tctttcgcat gattttcagc ttctttcgca
     9301 tcatgcacat ctttttcgac atcttcataa tccaagcgag taatttctcc gcctaattcc
     9361 atcattacca attgattcag tgggcgattg tcctcttcat cagcaatcaa gatcagtcct
     9421 gtagctcctt caccgatttt atttcctaca aagtcgaaca cggtctgggc atcttttatt
     9481 tctttggcat cttgtgtcgc accgatcatt cctccggcaa accatcctaa taaaatccct
     9541 agcggaccac ctaatacacc aaccaacatt ccgattaatc cacccgtaga agatttgtta
     9601 ttaccggtaa agtcgataaa atcttctatt ttgaattgat gtgtcccatt aggatcatgg
     9661 ctgacaactg ccatctgttc tcctttaatc gaacgaacag catgtaactt tttgatttct
     9721 gaaaatgcct gatatgcctg gctttctact tgaaaattta aaatgattac tcgtttgctc
     9781 attaaaaacg cccctttcat accttcttgt tatttatttt acctttaatt gaaaaataag
     9841 aaaagaaaag cactcttttt ttccaataaa atagggctgt gacaaaagtc gtatcacagc
     9901 tcctttttcc gaataaacgg cgttcttgaa gctgatcact tctgtcacaa cctcccttaa
     9961 atctgaatct atattttttg gtatttgttt ttatttgctt gcctattcga caaattcaaa
    10021 ttcaaactca ccgatacgta caaggtcgcc atctttcgca ccgcgtgccc gaagagcttc
    10081 atccacaccc ataccacgta attgacgagc aaaacgcatc actgtttcat catgatcgaa
    10141 attcgtcatt tggaataatt tttcgatctt ctcaccagat aagatccacg ttgcatctga
    10201 atcacgatcg atttggaact caggaccttc tggattgaat ccatagtgta cagtttcttc
    10261 ttccagttct tcttcgtaca atgggaattc tggcgttact tctaacaaat ctgctgtagc
    10321 attcaataac gcgtccaatc cttgtcgagt cactccagaa attggaaata ctgggatgtc
    10381 atcagcaaat tcatcttctt tttccttgtt cagctgttct ttgaatttca ctagattttc
    10441 cggtgcatcc ggcatatcca ttttgtttgc cacgatgatt tgcggacgct ctagcaagcg
    10501 taagttataa gtagataatt ctttgttgat ggcaagatag tcttcataag gatctcgtcc
    10561 ttccatccca ctcatatcga tcacatgtaa aattactcgt gttctttcga tatgacgaag
    10621 gaattgtgta cccagcccaa caccttgcga agctccttca atcaagcctg gcaagtctgc
    10681 tgcggcaaaa cttctgccat cacttgtagt aaccattcct agattaggta cgagcgtcgt
    10741 gaaatgataa gcgccgattt ttggacgagc agatgaaata acggatagta aagtcgattt
    10801 cccgacagat ggaaaaccta caagtccaac gtctgccaat actttcaatt ctagttcgat
    10861 ttttcgctct tgtcctggtt caccgttttc agcaatttcc ggtgcaggat tccgaggaga
    10921 ggcaaaacgg atattccctc gtccaccgcg tccacctttt gccacaacta gtgtttgacc
    10981 attttcaatt aaatctccta aaagagcacc agtctccgca tctcggacag tggttccttg
    11041 tggtacttta acataggtat cttctgaacc acgaccgtgc atccctttac tcatcccatt
    11101 ttcacctggc tgcgctttga aatggcgatt gaaccgaaaa tccatcaatg tacgtaaccc
    11161 ttcatcgacg atcaagatga catcgccgcc acgaccaccg tctccgccag ccggtccgcc
    11221 gtcaggaacg tatttttcgc gacgaaatgc aaccattccg tctccgccct tgccagcttt
    11281 cacatcgatc gttacttgat ctaaaaacat ggacataatt aagtcctcct attattcatt
    11341 tatttatttg actgcctgaa tttccacaat aaacaaaagt cttacttatt ttatcggtga
    11401 aatctgattt tgtctatcat ttcagcaaaa agattgttta ccttcttctt tttttatcat
    11461 ttggtaaggt ttccaacctt ttttctgcta ccaagtataa ttttttgaca aaatattctt
    11521 tgcgtagcag aataacagtg tattaattgt attaattgag gatgccgttt cacagcaccc
    11581 ctaataaagg aggaactttt gatatgacag taaaagtagg tattaacgga tttgggcgta
    11641 ttggccgtct ggctttccgc cgcatcaaag aagtctcaga tgatattgaa gtagtagcaa
    11701 tcaacgactt aacaagtcca actatgttag cacaattatt gcaatttgac tctactcatg
    11761 gtacttatcc tggcacagtt actgcaacag aagactcaat cgtagtggat ggcgaagcaa
    11821 ctcgtgttta tgcagaaccc gacgcaagca aaatcccttg ggcaaaagaa aacggtgtag
    11881 atatcgtact agaatgtact ggtttttata catctgaaga aaaagcaaaa gcgcatttag
    11941 atgctggtgt caaacgagta gttatttctg ccccagctgg tgctatgaaa accatcgtct
    12001 ataacgtcaa tgatgataca ttagatgaaa acgacaaaat catctcagca ggttcatgta
    12061 caacaaactg tttagcacct atggcttatt tcttaaataa tgaatttggg atcgaagttg
    12121 gtacaatgac aacagttcat gcctacactt ccacacaaat gctcttagat ggacctgtaa
    12181 aaggcggtaa tttgcgtgct gctcgttcag ctgctgacaa tactatccca cattctactg
    12241 gtgctgcgaa agcaatcggc ttagttatcc ctgagctaca aggaaaatta caaggacacg
    12301 cacaacgtgt tccagtcgtt gatggttcat taactgaatt ggtttcagta ttgaaaacaa
    12361 aagtaactgc tgatcaagta aacgaagcaa tcaaaaaaca cacagttgac aatccttcat
    12421 tcgggtatga tgaccgtcaa atcgtttcta gcgatgtcat tggaacaact caaggttcga
    12481 tctttgaccc aacgcaaaca gaagtaacaa ctgctggtga tttccaactt gttaaaactg
    12541 ttgcatggta tgacaatgag tatggattta cttgccaaat gattcgctta ctagaaaaat
    12601 tcgctaactt gtaatttgat agatcaatcg ataaaagaag cctttgactg gatccagtca
    12661 aaggcttctt tcttttcatc ccctactttt aaactcgttc aatgaacata ttggcttaaa
    12721 aattgttgga ctacttgctg atatttttca ggatctgtct tgtatgcttc cgcatgctcg
    12781 gctcctttta caacatactt ttcttttggt cccttcgttg cacgatagac cttatctaac
    12841 atttcaaatg gtacaaatgt atcttcgtct ccatgtataa agagcattgg gcgctggttt
    12901 ttcttcaatt gttccactgc atttgcttca ccgaaaaagt agccagcacg gacttttgtc
    12961 actagactcg ttacttgcat aagcggaaat gctggaagac taaacatatc gtctagctga
    13021 taagcaagct catcatgaac ggaagaataa ccgcaatctt caatgattgc tttcacatta
    13081 tctggtagtt tttctccgct agtcatcata actgttgctg ctcccatgct gacaccgtat
    13141 agaacgattt cttgcgattt tccattgttt tctagcactt tgtttatcca ctgtacatag
    13201 tctttacgct ctggccatcc aaatccaatg taatcccctt cgctttttcc atgtcctctt
    13261 gcatcaggaa caaggacatt gtaacccata tcatgaaaca tctttgcgta attggtcatc
    13321 gtttcagcgt ttcccatata gccatgagcc acaagagctg ttttttcact tttcgtttca
    13381 gcaggaagat aaatcgctga taatttcaga ccgtcttttg aagtgatggt ccaatcgctt
    13441 cggttttctt ttgaactaaa ccatttatgt gcatccgttt ctttggatgt ctgtgttgta
    13501 ccaggtgtat cttcgtctaa aaagtctttc tcagaaggca caacagcata attataaaaa
    13561 taattccctg caaacactaa cgcaacaatc agagctaaag cgacgcctaa aaggattttc
    13621 atccaaagtt tcataaatca tccatctcat ttctcttttt tcatataacc ttgaattagt
    13681 ataatcaaag aatctctact atgcaatgtt catttttata acccgcgaat tgtaaaatta
    13741 agttagaaaa ataaaaaggc atttatggta cactcaaatt gtatttccga cgaaagaaaa
    13801 caaagga
//
LOCUS       DACGLE010000070        13736 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0534
            SAMEA3304449-rid9560453.denovo.074, whole genome shotgun sequence.
ACCESSION   DACGLE010000070 DACGLE010000000
VERSION     DACGLE010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304449
            Sequence Read Archive: ERR985064
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13736)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13736)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702225.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702225.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 20:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,045
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 27 of 67
            Pseudo Genes (incomplete)         :: 25 of 67
            Pseudo Genes (internal stop)      :: 19 of 67
            Pseudo Genes (multiple problems)  :: 4 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13736
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304449-rid9560453.denovo.074"
                     /strain="VREN0534"
                     /db_xref="taxon:1352"
     gene            complement(63..1268)
                     /locus_tag="IXT20_002199"
     CDS             complement(63..1268)
                     /locus_tag="IXT20_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000398284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HAQ4860631.1"
                     /translation="MEGFLLNEQTWLQHLKEKRLAYGLSQNRLAVATGITRQYLSDIE
                     TGKVKPSEDLQQSLWEALERFNPDAPLEMLFDYVRIRFPTTDVQQVVENILQLKLSYF
                     LHEDYGFYSYSEHYALGDIFVLCSHELDKGVLVELKGRGCRQFESYLLAQQRSWYEFF
                     MDVLVAGGVMKRLDLAINDKTGILNIPVLTEKCQQEECISVFRSFKSYRSGELVRKEE
                     KECMGNTLYIGSLQSEVYFCIYEKDYEQYKKNDIPIEDAEVKNRFEIRLKNERAYYAV
                     RDLLVYDNPEHTAFKIINRYIRFVDKDDSKPRSDWKLNEEWAWFIGNNRERLKLTTKP
                     EPYSFQRTLNWLSHQVAPTLKVAIKLDEINQTQVVKDILDHAKLTDRHKQILKQQSVK
                     EQDVITTKK"
     gene            complement(1291..1444)
                     /locus_tag="IXT20_002200"
                     /pseudo
     CDS             complement(1291..1444)
                     /locus_tag="IXT20_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000879507.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
     gene            complement(1447..2832)
                     /locus_tag="IXT20_002201"
     CDS             complement(1447..2832)
                     /locus_tag="IXT20_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000813487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HAQ4860632.1"
                     /translation="MKQRGKRIRPSGKDLVFHFTIASLLPVFLLVVGLFHVKTIQQIN
                     WQDFNLSQADKIDIPYLIISFSVAILICLLVAFVFKRVRYDTVKQLYHRQKLAKMILE
                     NKWYESEQVKTEGFFKDSAGRTKEKITYFPKMYYRLKNGLIQIRVEITLGKYQDQLLH
                     LEKKLESGLYCELTDKELKDSYVEYTLLYDTIASRISIDEVEAKDGKLRLMKNVWWEY
                     DKLPHMLIAGGTGGGKTYFILTLIEALLHTDSKLYILDPKNADLADLGSVMANVYYRK
                     EDLLSCIETFYEEMMKRSEEMKQMKNYKTGKNYAYLGLPAHFLIFDEYVAFMEMLGTK
                     ENTAVMNKLKQIVMLGRQAGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSEMGYG
                     MMFGSDVQKDFFLKRIKGRGYVDVGTSVISEFYTPLVPKGYDFLEEIKKLSNSRQSTQ
                     ATCEAEVAGVD"
     gene            complement(2861..3247)
                     /locus_tag="IXT20_002202"
     CDS             complement(2861..3247)
                     /locus_tag="IXT20_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000985015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAQ4860633.1"
                     /translation="MMRLANGIVLDKDTTFGELKFSALRREVRIQNEDGSVSDEIKER
                     TYDLKSKGQGRMIQVSIPASVPLKEFDYNARVELINPIADTVATATYQGADVDWYIKA
                     DDIVLTKDSSSFKAQPQAKKEPTQDK"
     gene            complement(3263..3577)
                     /locus_tag="IXT20_002203"
     CDS             complement(3263..3577)
                     /locus_tag="IXT20_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006148869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAQ4860634.1"
                     /translation="MELKFVIPNMEKTFGNLEFAGEDKVVQRRINGRLTVLSRSYNLY
                     SDVQRADDIVVVLPAEAGEKHFGFEERVKLVNPRITAEGYKIGTRGFTNYLLHADDMI
                     KE"
     gene            complement(3930..4409)
                     /gene="rlmH"
                     /locus_tag="IXT20_002204"
     CDS             complement(3930..4409)
                     /gene="rlmH"
                     /locus_tag="IXT20_002204"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HAQ4860635.1"
                     /translation="MNIKIISVGKLKEKYLIQGINEYVKRLNAYAKIELIEVPDEKAP
                     ENLSEAQMRQVKEKEGERILAKIKEQEYVYGLAIEGKNPTSESFAKQIDQLGIQGKSH
                     LVFVIGGSLGLSEAVMKRSNAQISFGKMTYPHQLMRLILVEQIYRAFRINAGAPYHK"
     gene            4837..6126
                     /locus_tag="IXT20_002205"
     CDS             4837..6126
                     /locus_tag="IXT20_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HAQ4860636.1"
                     /translation="MDRKNVTPKMKKNKNNSLWRKLGLGLVGGIVGGLLTAGIFYAAM
                     GTGNNSSTATSGNQNSAGETVVENVKVNVDSDITKAVDKVQDAVVSVINLQSQNQSSG
                     FGGLFGQQEESSSSDDSNLEAYSEGSGVIYKKDGNTAYVVTNNHVVDGQQGLEVMMKD
                     GTKVKAELVGTDAYSDLAVLKINSDKVETVASFGDSSALKVGEPAIAIGSPLGSEYAN
                     SVTSGIISSLNRQVTSTNESNETVNINAIQTDAAINPGNSGGPLVNIEGQVIGINSSK
                     IASTSESTSNVSVEGMGFAIPSNDVVNIINQLEKDGKVTRPALGITMVDLSAVSTQQQ
                     EQILKIPSSVTNGVIVTNVQTATPAEKAGLKQYDVITKIDDKEVSSGVELQSALYKKK
                     VGDSVKITYYEGSKEKTTTVELTIDQSALKQNQNSEN"
     gene            complement(6165..6770)
                     /locus_tag="IXT20_002206"
     CDS             complement(6165..6770)
                     /locus_tag="IXT20_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4479 domain-containing protein"
                     /protein_id="HAQ4860637.1"
                     /translation="MIFAYNKAHVGDTLMVIIADDKGTENTVERKWNVARIKDEQGQV
                     VAWNFFHISDHLTIEGNGQVSLTDEELAKLNRLIKEAGFEEQLEADHEPKIVVGYVKT
                     CVPHPDSDHLSITETEVDNGTVLQIVCGAPNIEAGQKVVVAKPGAMMPDGMMIWPGTL
                     RGVASYGMICSARELHLPNAPEKKGILVLPEDAVVGEVFPK"
     gene            complement(6966..7430)
                     /locus_tag="IXT20_002207"
     CDS             complement(6966..7430)
                     /locus_tag="IXT20_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HAQ4860638.1"
                     /translation="MNQEYKNILVGIDGSEQALAAFKKALEVARRNNGKVYVANVIDQ
                     QFYNFMGYAPIDQNLVDQQTETAKQMIEECKEYGKSVDYTNIEGIIAYGSVRESMAVK
                     LPKKYAIDLIMVGQSGLNAVERFMTGSVASYVIRHSPCDVLIVSDEEEQPSN"
     gene            complement(7452..7772)
                     /locus_tag="IXT20_002208"
     CDS             complement(7452..7772)
                     /locus_tag="IXT20_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HAQ4860639.1"
                     /translation="MIIPNSLEELAGYVETGKSVFFFTAGWCGDCRFIKPQMPEIEND
                     FSDWQFIEVDRDKYIDVAAEWNIFGIPSFVVIQDGKELGRLVNKDRKTKQEIESFLES
                     VIKG"
     gene            complement(7915..8994)
                     /gene="pepA"
                     /locus_tag="IXT20_002209"
     CDS             complement(7915..8994)
                     /gene="pepA"
                     /locus_tag="IXT20_002209"
                     /EC_number="3.4.11.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl aminopeptidase"
                     /protein_id="HAQ4860640.1"
                     /translation="MEEKTFQRIKELTELQGTSGFEQDVRAYMESHMEPLVDELQLDG
                     LGGIFGLRHHEEADAPRVMVAAHMDEVGFMLTQIQENGLFKVVPLGGWNPYVVSAQRF
                     TLKTSTGKNYPCISSSVPPHLLRGTSGQKSVEVTDILFDAGFESREEAMSYGVLPGDT
                     IVPYAETIKTANGKNIISKSWDNRYGCTMVLEALEALQNETLGHTLIAGANVQEEVGL
                     RGSKASVNKFKPDLFFAVDCSAADDTVTKNGTFGHLGEGTLMRIQDPGLIMLPRLREY
                     LLDIAETNNIPYQYFVSKGGTDAGAAHTQNEGIPSTVIGVVGRYIHTHQTMFSIRDFE
                     AAREMLIQTLKGLDKSTVNTIVYGK"
     gene            9194..9544
                     /locus_tag="IXT20_002210"
     CDS             9194..9544
                     /locus_tag="IXT20_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HAQ4860641.1"
                     /translation="MYEESELSYFKGGLAVGIGLGLLSGVASTIWYHRKKVLSADQVL
                     ENIKTSFLKEGAIEGAWISFEKEPIRKFAVRSSAYRGGLSRIEDGETVYYEFLADAHT
                     GTVLDISRKKGAID"
     gene            9729..10682
                     /locus_tag="IXT20_002211"
     CDS             9729..10682
                     /locus_tag="IXT20_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAQ4860642.1"
                     /translation="MESREKIAVLIPCYNEEATISTVIEDFKRELPEADIYVYDNNST
                     DRTYELAVQGGAIVKKEPRQGKGNVIRQMFFDIDADYYLMVDGDDTYPAEAAHGLLEK
                     LRSGEADMVIGDRLSNGTYFDENKRPFHDFGNNLVKNTITYLYKTKIRDVMTGYRGFN
                     RIFVKSFPIMSSGFQIETELTIHALDKKFKFVEIPIDYRDRPEGSESKLNTFSDGFKV
                     IMMIIKMCKDYKPLLFFGIWTIFFFLFGLVMGIPVIREYMLTSFITRIPSAILSTGLM
                     TLALLSLVTGLILDTVVTNAKKEYELNLYHVYHEYQNKEMK"
     gene            10695..12482
                     /locus_tag="IXT20_002212"
     CDS             10695..12482
                     /locus_tag="IXT20_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4860643.1"
                     /translation="MHKKKENLFPTFFATLFASGFAFFTSYGLGTLNPTVIIGLAMVL
                     LLAFKQYGIELFSIKNKVAAIARLFFSAAFALTLSIGRQIQFQYVSANYTKTTIADGS
                     IFYFLVYFSIFVYSFLFLQSIYLSMPKLETFFTAQNTQAFSSRKYFLFFTVFLVLCWL
                     PYLLALYPGVVLPDSLSSVAQSMGDVPISNHHPVLFTLLVKLFVHDLPTDTINHGVFL
                     FSIFQTLITASAISYSLCWLLKRNIHFFPVLISLCYFAFAPVFPIYALNMQKDTLFSI
                     ACFLFSLAVFDFIFQREKPTILNQIYVIILALLTTYLRNNGIYVVTGTGLICGLIILK
                     QKRYQAFFQVVLGYTVIAGLLIHPLMARYAIPTETAEQLGVPLQQISRTIVMDGDLSK
                     KDKAFMNQLLPIKDYEAYSPSLADSIKWHKNFNNEFLNTHKKEFLSLWARNLPSNIKI
                     YTDAYILETFGFWVPGTKNSYGFLDTRVNTNNYGMKQVDLIEHLTGLSGMKKIIDARD
                     FFGSGTLLWLAIGSALLAIFKKKYRYLLLFLPGMLVFATIMIATPVAFSLRYVFVLAL
                     GLPVYILSPFLLDKREKMKTDIGDTHETI"
     gene            12469..13671
                     /locus_tag="IXT20_002213"
     CDS             12469..13671
                     /locus_tag="IXT20_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4860644.1"
                     /translation="MKLFKKIFFAFVLLIASYCVLLTITFFIPQHSIESNAEKSLQMV
                     EKEGMYPSINQGNMLGTRLDNFTDHLMIRKTKADPELNSLENAMSMADYPRYWHGYQL
                     FLRPLLLGMSLGSIRMIYAAILFLLIGLTSYFLTKCSDIYLTTALLISLAIGNAATFF
                     FSMQFSNIWILTLLSVLLFLTKPQLSNTNQQLFLYFFVIGSLANFFDLLTTPVISWGI
                     PVIIIYYVTNKYLMDKRSSLSKQVGSFVFTGIFWGLGYGLTWVAKWILSSIILNKNIV
                     KDAINQILFRTEGNDKYPLHRLEMLKSNIRLMYPKVAILLLLITCLVFLYFAYRKRNS
                     WRPFQLLVLFVLAAVTPYIWYNILANHSQIHYWFTYRTQIITSFAILSAFAFLIPPEK
                     SKDELNYF"
BASE COUNT         3938 a         2689 c         2520 g         4589 t
ORIGIN      
        1 aggttttgtc caaaattcat aaaaatgttc tcctctctat attcaatgaa tttgtatttg
       61 agttattttt ttgttgttat cacgtcctgt tcttttactg actgttgctt caaaatctgc
      121 ttgtgtcggt ctgtcagttt cgcatggtcg agaatgtctt ttacaacctg cgtctggttg
      181 atttcatcaa gtttaatcgc aacctttaag gtcggggcaa cttgatgaga tagccagttc
      241 agcgtccttt ggaaggagta aggctctggt tttgtggtta gttttaatcg ttcacgattg
      301 ttcccaataa accaagccca ttcttcattc agtttccaat cagaacgagg tttggaatcg
      361 tctttatcta caaaacggat ataccgattg ataattttaa aggcggtatg ctctggattg
      421 tcatagacga gtaaatcacg gactgcataa taggcacgct catttttcaa tcgaatctca
      481 aaacggtttt ttacttctgc gtcttcaatg ggaatatcat ttttcttgta ctgctcgtag
      541 tccttttcat agatacagaa ataaacttca ctttgtaatg aaccgatata gagggtgttt
      601 cccatacatt ccttttcctc tttgcgtacc agttcgccac tgcgatagct tttaaaactg
      661 cggaagacgg agatacattc ttcctgttgg cacttttcag tgagtacagg gatatttaaa
      721 atccctgtct tatcgttaat ggcaaggtca aggcgtttca tcacaccgcc agccaccaaa
      781 acgtccataa agaactcata ccagcttctt tgttgtgcca gaagatagct ttcaaattgt
      841 ctgcacccac gacctttcaa ttccaccaga actcctttgt ccagttcatg ggagcaaagg
      901 acgaatatgt cgcctaaagc ataatgctct gaataagaat agaaaccata gtcctcatga
      961 agaaaatagg acagtttcag ttgtaagatg ttttcgacca cctgctgtac gtctgttgtc
     1021 ggaaagcgaa ttcttacata atcaaacagc atttcaaggg gagcgtcggg attgaagcgt
     1081 tccagagctt cccaaaggga ctgctgtaaa tcctctgatg gcttgacttt tcctgtttca
     1141 atatcgctta gatactgcct tgtaatacca gtcgcaacag ctaaacggtt ttgagatagt
     1201 ccataagcca agcgtttttc ttttaaatgc tgtaaccaag tttgttcatt cagtaaaaat
     1261 ccctccaatc aaaaaggcgt atgtcaactt ttaaagccca tttgacatac gctgaaattt
     1321 tgtaaatccc ttgtaaccaa aggattttct aatgtttttt tgactgtttc ctgtcgattt
     1381 gtacccccct gttagatacc gaggggttaa gtgctggcgt ggctattgcc acaccagcca
     1441 gcaagatcag tccacacctg cgacttccgc ttcgcacgtc gcctgcgtgg actgtctgct
     1501 gttggataac tttttaattt cctccaagaa atcatatcct tttggtacaa ggggagtata
     1561 aaactctgat atgacacttg ttcctacatc aacatagcca cgacctttga ttcgctttaa
     1621 gaagaaatcc ttttgtacgt cactgccaaa catcatgcca tagcccattt cagacatacg
     1681 acctaaagcc actctgaaat taaactgatc acggattccg tcgcctaaat attttgcgtc
     1741 tggacgttga caagccagta ttagaaagaa gccagcttga cgacctaaca tgacaatctg
     1801 tttcagctta ttcataactg cggtgttttc ttttgttccc agcatttcca tgaaagcgac
     1861 gtattcatca aagattaaga agtgtgccgg gagacctaag taagcataat ttttgccagt
     1921 cttatagttc ttcatctgct tcatttcctc actacgtttc atcatttctt catagaatgt
     1981 ttcaatgcaa gaaagcaagt cttcttttct atagtagaca tttgccatca cagaacctaa
     2041 gtccgcaagg tcagcatttt tcgggtcaag aatatacagt tttgaatctg tatgaagcaa
     2101 ggcttcaatc agtgtcagta taaagtaagt tttaccgcca cctgtaccac cagcaatcaa
     2161 catatgaggg agcttatcat attcccacca tacgtttttc attaagcgaa gtttaccatc
     2221 tttagcttct acttcatcaa tagaaatacg actggctatg gtgtcataga gcaaagtata
     2281 ttccacatag gaatccttta actctttatc cgtcagctca cagtacaagc cactctctaa
     2341 tttcttttcc aagtgtaaga gttggtcttg atattttccc agcgtgattt ccacccgtat
     2401 ctgtatcaag ccatttttaa gtcgataata cattttaggg aagtaggtta tcttttcctt
     2461 tgtacgacca gcactatctt taaagaaacc ctctgttttg acctgttcag attcatacca
     2521 cttgttttca agtatcatct ttgccagttt ttgacggtgg taaagttgtt taaccgtatc
     2581 atagcgaacc cgtttgaata caaacgctac cagcaagcag ataagaattg cgacactgaa
     2641 actgataatt aaatagggaa tgtcaatctt atctgcttgt gataggttaa aatcctgcca
     2701 gttgatctgc tggattgtct tcacatgaaa cagtccgaca accagcagga aaacaggcag
     2761 gagtgacgct atcgtaaaat gaaagactaa atctttacca gatgggcgaa tccttttacc
     2821 acgctgtttc atgcgaaaaa gtctcctttc tacctagcga ctatttgtct tgtgtcggtt
     2881 ctttctttgc ttgtggttga gctttgaatg aactagaatc ctttgtcagc acaatatcgt
     2941 ctgccttgat ataccagtca acatctgctc cttgataggt ggcagtagca acggtgtccg
     3001 caatgggatt gataagttcc acccgtgcgt tataatcaaa ctctttcaaa ggcacgctgg
     3061 caggaatact tacttgaatc atgcgtcctt gtcctttgga ttttaagtca taggtacgtt
     3121 ccttgatttc atctgaaacc gacccgtctt cattttggat tctcacttca cgacgtagag
     3181 cagagaattt caattctcca aaagtcgtgt ctttatctaa tacaatgcca tttgctaatc
     3241 tcatcatttt tcctctcttt ctttattctt ttatcatgtc gtcagcatgt aaaaggtaat
     3301 ttgtaaaacc acgagtgccg attttgtagc cctctgcggt aatacgtgga ttgactaact
     3361 tcacacgttc ctcaaagccg aaatgttttt cgccagcttc agcaggaagc accaccacaa
     3421 tatcatctgc tctttgaaca tcagaataga gattatagct tcttgataag acagttagcc
     3481 gtccgttgat tcttcgctga acgactttat cctcgccagc aaattctaaa ttgccgaatg
     3541 ttttttccat gttgggaatc acaaatttaa gttccatatt tttacctatc ctttcttttt
     3601 tattggctga atgaatgttt gatggtctta aagagtgggg aacgaccttt tgattcttga
     3661 ttttttgttt tcataagttc acttcctttc aaaatcgggt aaaaaaatag acacctcatt
     3721 ttttgaagtg tctacctatt aaatattcaa attttattgg aagtatcttt atatcttcac
     3781 ttttcaagga taaatcgtcg tatcaaagct cattcataag tagtaaatta gtagtaaatt
     3841 gagtggtttt gaccttgata aagtgtgata agtccagttt ttatgcggat aactagattt
     3901 ttatgctatt tttctcttaa aaaatatcat tatttgtggt atggagcccc cgcgttgatc
     3961 cggaaggcac ggtagatttg ctcgacgagg atcaggcgca tcaattgatg tggatacgtc
     4021 attttgccaa aagagatctg agcattgctt cttttcatga cggcttcgct gagtcctaaa
     4081 gaaccgccga taacaaaaac aaggtggctt tttccttgga tacctaattg gtcgatttgt
     4141 ttagcaaaag attcgcttgt gggatttttc ccttcaattg ctaagccata gacgtattct
     4201 tgttctttga ttttcgctaa gatccgttct ccttcttttt cttttacttg tctcatttgc
     4261 gcttcgctta aattctccgg ggctttttcg tcaggaactt cgatcaattc gattttcgca
     4321 taggcattca gccgtttgac atactcattg ataccttgga tcaagtattt ttcttttaat
     4381 tttcctactg aaataatttt gatattcatg ttttctctcc tttgatcttc acgctcactt
     4441 tgtttattat aatcaatttt ttcgtctata tgtttatttt gactattttt gttaaaaaga
     4501 tttccccaac agttatgcac aacatccaca ggtttatcca cagaaaacag cgttgtttta
     4561 ccttatttca tcacattcat taggcaaaca tacgttttat aagaatttat ccactttata
     4621 cacaggctgt ggatatgttt tatcccttat ttttccaatc ctatccgcgt agttttacac
     4681 aatatctgtg gaaaaagaaa atcaaatatt ttcatctgaa aacaatgatt tttcctaaag
     4741 tttttcataa aaagttcaaa gtctcaaagt agactaaagt caacaaataa gactatacta
     4801 acagtagata aatgaatgta attggaggag gaacacatgg atagaaaaaa tgtgacacca
     4861 aagatgaaaa aaaataaaaa taatagcctc tggcgtaagt tgggtctggg gcttgttgga
     4921 gggatcgttg gaggactact gacagctggc attttttatg cagcgatggg cactggcaac
     4981 aatagttcta ctgctacaag cggcaatcaa aattctgccg gtgaaacagt tgtggaaaat
     5041 gtcaaagtca atgtagattc tgatatcacc aaagcggtag ataaagttca agacgctgtc
     5101 gtttctgtta tcaacttaca aagtcaaaac caatcaagcg gttttggcgg attattcggg
     5161 cagcaagaag aaagtagcag ctcagatgat agtaatttgg aagcttatag tgaaggaagc
     5221 ggcgtaatct acaaaaaaga cgggaatact gcttatgtag tcacaaacaa tcacgtagta
     5281 gatggacagc aaggtttgga agtcatgatg aaagacggta cgaaggtaaa agctgaatta
     5341 gttggaacag atgcctattc agatttagcg gtgttgaaga tcaattcaga caaagtcgaa
     5401 acggttgcct cttttgggga ctcaagtgct ttgaaggttg gtgaacctgc gattgcgatt
     5461 ggttccccat taggatctga atatgcgaat tctgtcactt caggaatcat ttcttccttg
     5521 aaccgacaag taacaagtac gaatgaatcg aacgaaacag tcaatatcaa tgcgatccaa
     5581 acagatgcag cgatcaaccc gggaaattct ggtggtcctt tagtgaatat cgaaggacaa
     5641 gttatcggta tcaactcaag taagatcgca agtacttctg aatcgacttc taatgtaagc
     5701 gtagaaggaa tgggatttgc catcccaagt aacgatgtag tgaatatcat caaccaactg
     5761 gaaaaagacg gaaaagtgac acgtccagct ctaggtatca caatggtcga cttatctgcc
     5821 gtttctactc agcaacaaga acaaatcttg aagattcctt catctgtaac taacggtgtc
     5881 atcgtgacaa atgtccaaac agctacaccg gcagaaaaag ctgggttgaa acaatacgac
     5941 gtcattacta agatcgatga caaagaagtt tcttctggtg tagaacttca atctgctttg
     6001 tataagaaaa aagtgggaga ttctgtcaaa atcacttact atgaaggctc gaaagaaaaa
     6061 acaactactg ttgaattgac gatcgatcaa tcggcattaa aacaaaatca aaatagtgaa
     6121 aattaataaa aaagaggttg tgacaaagac ttgtcacaac ctctttattt tggaaatact
     6181 tctccgacta cggcatcctc aggcaatact aagattcctt ttttttctgg agcatttggc
     6241 aaatgcaatt cacgagcaga acaaatcatt ccatagctgg ctacccctcg caaagttcct
     6301 ggccagatca tcattccatc tggcatcatg gctcctggtt ttgcaacgac aactttttgt
     6361 ccagcttcga tatttggtgc accgcaaaca atttgcaata ctgtcccatt gtccacttct
     6421 gtctcagtaa tcgataaatg gtcagagtca ggatgcggga cgcatgtttt tacatacccg
     6481 acaacgatct tcggttcatg atcagcttca agctgttctt cgaatcctgc ttctttaatc
     6541 aatcgattca atttcgctaa ttcttcatct gttaagctta cttggccgtt cccttcaatt
     6601 gttagatgat cggagatatg gaaaaagttc caagcaacaa cttgaccttg ttcatctttg
     6661 atccgagcaa cattccattt acgttcgaca gtgttttctg ttcctttatc gtcggctatg
     6721 ataaccatca aagtgtctcc gacgtgtgct ttgttataag caaaaatcat gttctttcct
     6781 ccattatcct ttgctgagat ttatcttaac aaaaaattgc attcactgca tgagattttt
     6841 ttcatgcttt atttccgaag aagcttcttc tggaacacca tttattcgta acaagggact
     6901 gtgacaagac ttttgtcaca gtccctttac tgtttgatta aaaaggttat acaagttcca
     6961 cttgcttaat tactcggctg ttcttcttcg tcagaaacga tcaagacatc acaaggtgaa
     7021 tgacgtatga cgtagctggc tacacttcct gtcataaagc gttcgactgc atttagtcca
     7081 gactgcccaa ccatgattag atcgattgca tatttttttg gcaatttgac tgccatggat
     7141 tcacgaacag agccgtatgc aataattcct tctatatttg tgtaatcaac agattttcca
     7201 tattctttac attcttcgat catttgcttg gctgtttcag tttgctgatc gactagattt
     7261 tgatcaatag gagcatagcc cataaaatta taaaattgct gatcgattac gtttgctaca
     7321 tatactttcc cattattacg gcgagccact tctaacgctt ttttgaaagc agcaagtgct
     7381 tgttcactac cgtcaatacc tactaaaatg ttcttatatt cttgattcat cttgacccct
     7441 cctaacagat gtcagccttt tattacactt tctaggaacg attcgatttc ctgttttgtt
     7501 ttacgatctt tattcactag acgacctaat tcttttccgt cttggatcac gacaaaactt
     7561 gggataccaa aaatgttcca ttcagcggct acatctatgt acttgtcacg gtctacttca
     7621 ataaattgcc aatcagaaaa gtcattttcg atttcaggca tttgcggttt gataaaacgg
     7681 cagtctccgc accagccggc agtaaagaaa aatacacttt ttccagtttc tacgtagcct
     7741 gctaattctt ctaaactatt tgggataatc attctttttc ctctactttc tgtttatgta
     7801 tttttcaatt aaattataca cctttctttc gaactaacaa acattgatcc tctttttata
     7861 aaaaaaatct gacaaaaggt tgcttttgcc agattttata gtttcactat cgcgttattt
     7921 cccgtatacg atcgtgttca ctgttgattt atctaaacct ttcaatgttt gaatcaacat
     7981 ttctcgtgca gcttcaaaat cgcgaatact gaacatcgtt tgatgcgtat ggatataacg
     8041 tcccactacg ccaatcactg tacttgggat tccttcattt tgtgtatgag cagcacctgc
     8101 atctgtaccg ccttttgaaa caaagtattg ataagggatg ttgtttgttt cagcgatatc
     8161 tagcaagtac tcacgcaaac gaggaagcat gatcaaacct ggatcttgga tacgcatcaa
     8221 tgtaccttct cctaaatgtc cgaatgtgcc atttttcgtc actgtgtcat ctgctgcaga
     8281 gcagtctact gcaaagaaca gatctggctt gaatttattg acagaagctt tagaaccacg
     8341 caagcctact tcttcttgga cattcgcgcc tgcaatcaat gtatgaccta atgtttcatt
     8401 ttgtaacgct tctaaagctt ctaataccat tgtacagcca tagcggttgt cccacgattt
     8461 gctgatgata ttcttcccgt tggctgtttt gatcgtttca gcgtaaggaa cgattgtgtc
     8521 tcctggaaga acaccgtaac tcattgcctc ttctcttgat tcaaacccag catcgaacaa
     8581 aatatctgtt acttctactg atttttgtcc gcttgttcct cgtaataaat gtgggggaac
     8641 ggaagaagaa atacatggat aattcttacc agtagaggtt ttaagtgtaa aacgttgagc
     8701 agaaacaacg taagggttcc atccgcctag aggtacgact ttaaaaagcc cgttttcttg
     8761 gatttgtgtc agcatgaatc cgacttcatc catatgtgct gcgaccatta cacgaggtgc
     8821 atccgcttct tcatggtggc gcagcccaaa aatgccgccc aaaccatcta actgtaactc
     8881 atctaccagc ggttccatat gactttccat ataggcgcga acgtcttgtt caaaaccgct
     8941 tgttccttgt aattctgtta actctttgat tcgttgaaat gttttttctt ccatatgcta
     9001 ttcgtctaaa tttcagacta aactcctctc agacttatat tgatctgcgg ataaaatatg
     9061 attttttctg cagaccgatc gtatttatta tagcttattt tttctggtag cggttattta
     9121 tttcaaagaa aatatgagag tgtggtaaaa taaacacaaa gagtagcaag tgagaagaag
     9181 gaggtatatc cacatgtatg aagaaagcga attaagctat tttaaaggcg ggttagccgt
     9241 tgggatcggt ctaggattat tgagtggggt agcttcaacg atttggtatc atcgcaaaaa
     9301 agtgttatca gcagaccaag tgcttgaaaa tatcaaaaca tcttttttaa aagaaggcgc
     9361 aatcgaaggt gcttggatct cttttgaaaa agaacctatt cggaaattcg ccgttcggtc
     9421 gagtgcttat cggggtgggc tttcccggat agaagatggc gaaactgttt attatgaatt
     9481 tttagcagat gctcatacag gaaccgtatt agacatttca agaaaaaagg gcgcaataga
     9541 ttaatacgtc aaaaaaatga aataacgaat acgcacccac tatctaaaag agaggtatcc
     9601 tatccttctt ttatagtggg tgatttttat aaaaataggg ctatttcctc ttggaatagc
     9661 tagtatttgt agttattata cggtattctt gaaataagat tagttttatt atataaggag
     9721 agtgacaaat ggaatcacgt gaaaaaattg ccgtgctcat tccttgttat aacgaagagg
     9781 cgacaatttc aactgttatt gaagatttta aaagggaatt accagaagca gatatttatg
     9841 tctatgataa taattcgacc gatcgaacat acgaattggc cgttcaaggc ggagcaatcg
     9901 taaagaaaga accacgtcaa ggaaaaggga atgtaatccg tcaaatgttt tttgatattg
     9961 atgccgatta ttatttgatg gtagatggtg atgatactta tcctgcagaa gctgcacatg
    10021 gtttgcttga gaaattacgt tctggcgaag cagatatggt cattggcgat cgtctatcaa
    10081 atggaaccta ttttgatgaa aataagcgtc ctttccatga ttttgggaat aatctggtta
    10141 aaaatacaat tacttactta tataaaacaa aaattcgcga tgtcatgacc ggctatcgag
    10201 gattcaatcg aatttttgtc aaaagcttcc ctattatgag ttctggtttt caaatcgaaa
    10261 cagaattgac gattcatgct ttagataaaa aattcaagtt cgttgagatt ccaatcgatt
    10321 atcgcgatcg tcctgaaggt agcgaatcaa aattgaatac attctctgac ggattcaaag
    10381 tgatcatgat gatcatcaaa atgtgcaaag attataaacc tttgcttttc tttgggatct
    10441 ggaccatctt cttcttttta tttggattgg tcatggggat tcctgtgatt cgtgaatata
    10501 tgctgacaag ctttattaca cgcatccctt ctgcgatttt aagtactgga ttgatgactt
    10561 tagcccttct ttcattagta acagggttga ttttggatac ggtcgtcaca aatgccaaga
    10621 aagaatatga attgaattta taccatgtct accacgaata tcaaaataaa gaaatgaagt
    10681 aggaggcctt ttttatgcat aagaaaaagg agaatctatt tcctactttt tttgcaactt
    10741 tatttgcgtc aggttttgct ttttttactt cttatggtct aggaactttg aatccaacag
    10801 taatcattgg tttagccatg gttttgctac ttgcttttaa gcaatatgga atagagttgt
    10861 tttcaataaa aaacaaagtt gcagctattg cccgattgtt tttttctgca gcttttgctc
    10921 ttacactttc tattggcaga cagatacaat ttcagtatgt ctcagcaaac tatacaaaga
    10981 caacgattgc tgatggaagt attttttact ttcttgttta tttctccatt tttgtgtatt
    11041 cttttttatt tttacaatcg atttatttat caatgccaaa actggaaaca ttttttactg
    11101 ctcaaaacac acaggctttc tcttcaagaa aatatttttt attctttact gtctttttag
    11161 ttctgtgttg gcttccttat ctgttggcat tgtatcctgg agtggttttg cctgattcgt
    11221 taagttcggt cgcccaatct atgggtgatg ttcctataag caatcatcat ccagtcttat
    11281 tcaccttact ggtcaagcta tttgttcatg atttaccaac tgatacgatc aatcacggtg
    11341 tatttttatt ttcgattttt caaacattga taacagcttc agcgataagt tattctcttt
    11401 gctggctgtt aaaaagaaac atccattttt ttccggtact cattagttta tgctattttg
    11461 catttgcacc ggtttttcca atctacgcct tgaacatgca aaaagatact ttgttctcta
    11521 tcgcttgctt tttattttcc ttagcggttt ttgactttat ttttcaacga gaaaagccaa
    11581 cgattctcaa tcagatttac gtaatcatcc ttgctttact gaccacctat ctaagaaata
    11641 atgggattta tgtagtaacc ggcacaggtt tgatttgcgg cttgattatt ttgaaacaaa
    11701 aaaggtatca agcattcttt caagtggtgc tgggttatac agtgattgct ggactactca
    11761 tccacccgtt gatggctcgt tatgctattc ctacagaaac tgctgaacaa ttaggtgttc
    11821 ctttgcagca aatttctaga actattgtta tggatggtga cttgtctaag aaagataaag
    11881 cattcatgaa tcagctactc ccgataaaag attatgaagc gtattctcct agtcttgcag
    11941 actcaatcaa gtggcataaa aattttaaca atgagttttt gaatacacat aaaaaagaat
    12001 ttctatcact atgggcaaga aatttaccaa gtaatataaa aatctataca gatgcttata
    12061 ttttagaaac gttcgggttc tgggtacctg gtacaaaaaa tagttacggc tttttagata
    12121 ctcgtgtaaa cacgaataat tatggtatga agcaagtaga tttgattgaa catcttactg
    12181 gattgtcagg aatgaaaaaa atcatcgatg ctcgtgattt ctttggttcc ggcacattac
    12241 tgtggttagc tatcggcagt gcacttttag caatctttaa gaaaaaatat cggtatttac
    12301 tcctttttct tcccggtatg cttgtttttg ctactattat gattgctact cctgtggctt
    12361 ttagtttacg gtatgtgttt gttttagcat taggtctacc tgtttatatc ctctctcctt
    12421 ttttactaga taaaagagaa aaaatgaaaa cagatatagg agatactcat gaaactattt
    12481 aaaaagatat tttttgcttt tgttctcttg atcgctagct attgcgtact actgacaatt
    12541 acttttttca ttccgcagca ctctattgaa tcgaatgcag aaaaatctct acagatggtt
    12601 gaaaaagaag gaatgtatcc ttctatcaat caaggaaata tgctaggcac tcggttagat
    12661 aattttactg atcatttgat gatacgaaaa acaaaagcag atcctgaatt gaactccttg
    12721 gagaacgcga tgtctatggc ggattatccc cgttattggc atggttatca gcttttttta
    12781 cgacctttac tgctagggat gagtttgggg agtattcgaa tgatttacgc agccattctt
    12841 tttctgctga ttggattgac gagctacttt ttaaccaagt gtagcgacat ctatttaacg
    12901 acagccttgt tgatttcgct tgctatagga aatgctgcaa cctttttctt cagtatgcaa
    12961 ttttcaaata tttggatttt gactttattg tctgtattac tatttttaac aaaaccacaa
    13021 ctctccaaca ccaaccaaca attattcttg tatttctttg tcattggttc tttagctaac
    13081 ttttttgact tgttgacaac accggtcatc tcttggggga tacctgtgat cattatttat
    13141 tatgtaacaa ataagtattt aatggataaa cgcagttcat tgagcaaaca agtaggcagt
    13201 tttgttttta ccggtatctt ttggggatta ggctatggtt tgacatgggt ggctaaatgg
    13261 atcctatcat cgatcatatt gaacaaaaac attgtaaaag atgcaatcaa ccaaatcctc
    13321 tttcgaacgg aaggaaacga caaatacccg ttgcatcgat tagagatgct gaaaagcaat
    13381 attagattga tgtatccaaa agtagcaata ttattgctat tgattacttg tcttgttttt
    13441 ctctattttg cttatcgtaa acgaaatagc tggcgtccgt tccaacttct agttttgttt
    13501 gttttagctg ctgtaacgcc gtatatttgg tacaatattt tagctaatca ctcacagatt
    13561 cattattggt ttacgtatcg tacgcaaatc atcacaagtt ttgctatact tagtgctttc
    13621 gcctttttga tccctcctga gaaatcaaaa gacgaactaa attactttta agaataccga
    13681 tgtactaatt catttttaaa cataaaaact ccagctgtca gagatagccg gagttt
//
LOCUS       DACGLE010000071        13700 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0534
            SAMEA3304449-rid9560453.denovo.075, whole genome shotgun sequence.
ACCESSION   DACGLE010000071 DACGLE010000000
VERSION     DACGLE010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304449
            Sequence Read Archive: ERR985064
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13700)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13700)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702225.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702225.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 20:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,045
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 27 of 67
            Pseudo Genes (incomplete)         :: 25 of 67
            Pseudo Genes (internal stop)      :: 19 of 67
            Pseudo Genes (multiple problems)  :: 4 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13700
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304449-rid9560453.denovo.075"
                     /strain="VREN0534"
                     /db_xref="taxon:1352"
     gene            577..1473
                     /locus_tag="IXT20_002214"
     CDS             577..1473
                     /locus_tag="IXT20_002214"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4860645.1"
                     /translation="MTKYIMVIFSLVVLTACSKGEDSTVVESTSTDSTTVETSVTSTT
                     KSSTEEISTSSTETTQDFPYAVNLNDFIQEVKPNGNEANKKTISHLTFVTNQKNVPTT
                     ITLNTKDLNDFGNGIYISSDTEEVFYPVSINEIPTQNIILVGDNGEEREVKVNTEVKI
                     EVPNDDQSDSFQIVGDSYYLFYNQQETISLATRNFDENPGSKDLDNSNMIEYVQQVDS
                     QNTEDSEPTENTKSDEYYHSIKEAWNKQKDYVDGIEDPKIKQATQTPFSAANAEATRL
                     EQENPEDAEVIKASLKRVLDGE"
     gene            1485..2405
                     /locus_tag="IXT20_002215"
     CDS             1485..2405
                     /locus_tag="IXT20_002215"
                     /inference="COORDINATES: protein motif:HMM:NF019556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4860646.1"
                     /translation="MSGYDGHPEIELALGRLYELTQKESYLTLAKFYIDERVQDDFFD
                     RQIEADGFERDVIDGMRNFPLSYYQAAEPIREQETAEGHAVRVVYLCTGMAKVGRYTN
                     DESLLKACKRLWNNIVEQKMYITGGVGSTNIGESFTGNYDLPNDTMHGETCASVGMAF
                     FAKEMLELESKGEYADVLEKQLFNGIISGMSLDGAHFFYVNPLQADPEVSEHNPSRNH
                     VLTDRAEWFGCACCPSNVARLVASVDHYIYTVKDTMILSHQFISNDATFESGVTIIQT
                     SNYPWEGQIYYEINNPNDVETTLGIRIPAW"
     gene            2448..2876
                     /locus_tag="IXT20_002216"
     CDS             2448..2876
                     /locus_tag="IXT20_002216"
                     /inference="COORDINATES: protein motif:HMM:NF019556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4860647.1"
                     /translation="MKVENGFIYFKLLAGSFEILLTLDMVVKKYQANQQIAHDFGKVA
                     IQRGPIVYCVESMDQTEDVWKYQLIQAGEIAYAFDDKLLNGVGVIRAEAKVANGRSAS
                     VYQEYQPLTRRAAELKLIPYYAWANRGKNQMLVWIDQNDC"
     gene            complement(2972..3841)
                     /locus_tag="IXT20_002217"
     CDS             complement(2972..3841)
                     /locus_tag="IXT20_002217"
                     /inference="COORDINATES: protein
                     motif:HMM:NF014376.1,HMM:NF024242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAQ4860648.1"
                     /translation="MEIENLNLYRDSAWNDLKIRFCGHSECLPLHSYGPGSRPYYLLH
                     FVLSGKGKLLVNKTEYQIEAGQLFLVEPDQMVFYQADEDTPWTYSWVGFNGGLASHFI
                     NRLGLGYPTLTRDIEPDTFIEIKKLLENIIKSNINNTVNDLYINGQFLILLSKIGTDE
                     FGKEMIIDEEKYSAEKYVQEAIMIVEDFYGTELTVNDIANRLNINRSYLSSIFVKHVG
                     TPLKKYLVEFRISQSEEFLFTTNWSVDYISKVCGFNNPSYFSKIFKKFHGLSPTEYRE
                     QRHLRRSQTVFID"
     gene            4222..5484
                     /locus_tag="IXT20_002218"
     CDS             4222..5484
                     /locus_tag="IXT20_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006427715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 27 protein"
                     /protein_id="HAQ4860649.1"
                     /translation="MRNLRNFAKTPPKGWNSWDVYGASVTEEEVRRNADVMATKLKDH
                     GWDYVVVDIQWYEPTADSAAYHVFAPLNIDEYSRVMPAENRFPSAKDGNGFKDLGDYI
                     HSLGLKFGIHILRGIPREAVHKQMKIKGTTKTADQIAFNSICPWNSDMYGVNVNIPEG
                     QMYYDSILEMYAEWGVDFIKVDDIAYSTIYREAHLAEIAAIRKAIDKTGREIVLSLSP
                     GPARLQDGSFLQENANMWRLTDDFWDDWELLYDMFDRCNYWSPFIRPGNWPDCDMLPL
                     GHIGIRSGERGFPDRNTNFTKPEQKTMITLWSIFQSPLMYGGELADLDDWTLSLLTNE
                     EINSMHSSLTKQKQESRNENWIVWSGENEKDKYIAIFNVSNNEQDIPEEILNEYIQTE
                     NAKELWSGDSKDAFNKTLESHDCAIFKL"
     gene            5654..7030
                     /locus_tag="IXT20_002219"
     CDS             5654..7030
                     /locus_tag="IXT20_002219"
                     /inference="COORDINATES: protein motif:HMM:TIGR00792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ4860650.1"
                     /translation="MDTIGKSLGLKEKMAYGCGDAASNIIWASMGAFMMFYYTDVVNI
                     SAATIGTIFLISRVINAVVALATGWLIDNTKSKYGKARPWLLWFAVPFAIVTFAIFSV
                     PDIAKLYQVIYVFITYNAMMTVYSLINIPYGSMATMMTKDQYQRTLLNIFRQLFAQIA
                     SLLVTAFTLPLVKVFSSSVGEKNAWSVVYGIFGIAAGVLFFLCFIGTKERIHDNEVVK
                     EEKINIVKGMKSVLKNNYWVMLMVISISINIFFQAISTVNTYYCKAVLHDSNMIGILN
                     TAYIVPAIVAFFFVHLIVKKFGKGYTTMFGWIIICASYAVLLPFTENHMALIITSAMR
                     GVGYCFLLAVSSAMVSDAVEYGEWKTHVRVEGLTFSMQGFVTTIAAGLVTAFIGGVLD
                     FSKYDGTLTFAQAQPVTAVTAIKLLFIAMPFIVGALNVLLLKFYKLDKMYPKIIEDLN
                     MRNSVTTK"
     gene            7158..8567
                     /locus_tag="IXT20_002220"
     CDS             7158..8567
                     /locus_tag="IXT20_002220"
                     /inference="COORDINATES: protein motif:HMM:TIGR00792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ4860651.1"
                     /translation="MKSIISDEKECSKEEKLSSGFKTSITEKIAYGMGDVACNVVYAL
                     TSGLLVYFYTNVIGVPAGVIGTILLLSRIFDGISDLLIAQLMDKINSKHGKARAWVLW
                     MSIPYALTAVALFLVPANATLMVQTVYIFITYNLCTTVVYTGLNLPYSAMAPLMTNDD
                     DDLAKLNLFRMSMSPISNLIVTAVSLPFINMLGGDQRAWIIVTAVYGVVAFGMLIWTF
                     LGTKERFHTSAAAEAEELPFSQRLKATGKNKYFIIMFLTVICLSIYQNVNGTVSTYYA
                     QYILGNAELMGLMQTFEKVPWILGIMFMAPLIRRFGKRNLVLFGAILCVGAQVLIMFN
                     PSNLNLILFSAVLRGLGEAPINGLTFTMIADVINYGHWKTGIRVHALIFSTFTVGQKF
                     GGGITGWAIGQLLEVSGFSGSVTEIPSAVSMVQNLYIYGTILAWALVAVCMLKYHLDK
                     EYDEIMVDLKNREGDKLTV"
     gene            8684..9763
                     /locus_tag="IXT20_002221"
     CDS             8684..9763
                     /locus_tag="IXT20_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ4860652.1"
                     /translation="MSKSKYQIIAEEIKGKIESNEYGFNQTIPPEMQLQKDYAVSRHT
                     VRQAIAVLVNEGYLRKEKGSGTYVDNRFKNDSKVKATPKTIGVITTYLSDYIFPSIIR
                     GIEGTLRSKGYSLLLASTNNDYQQEKECLEKMINYGVQGLIVEPTKSNQYNPNLALYV
                     SLKEQGIPMVMINAVYEELEAAYICVNDVRSGFLATELMIKKGNKNLLLVSKIDDLQG
                     KYRMKGFIQACEKYGVKIQSQDIITYTTDTRKQVSSIVLKRLKEEKKINGIVCYNDQI
                     ASVIVDNLNSQEISVPDDISIIGNDDSSLSKVGSIKMTTLKHPKVEMGKDAAEWIIKT
                     IEKNSEQKSILYEPILIERDSVLNR"
     gene            9915..11504
                     /locus_tag="IXT20_002222"
     CDS             9915..11504
                     /locus_tag="IXT20_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HAQ4860653.1"
                     /translation="MTKADIMLDIIDGRTSLGIEFGSTRIKAVLVASDLSPIAAGSYE
                     WENQLENGIWTYSLDDVWKGLRISYRKMAAEVFEEYGETLSTIGSLGFSAMMHGYMAF
                     DAENDLLVPFRTWRNAITEEAEEKLTKAFRFNIPQRWSIAHFYQAILNKEKHVGNVTF
                     FTTLAGYIHWQLSGEKVLGVGDASGMFPIDVAIKDYDQQKIAIFNNLVKKEDMDINIK
                     SFLPRVLLAGEAAGNLTQEGAYLLDPTGTLKAGIPLCPPEGDAGTGMVATNSVAKRTG
                     NVSAGTSAFAMIVLEEDLKEVHPEIDLVTTPSGEHVAMVHTNNCSSEINSWIKIFKEF
                     TETLGFEISTESLYDKLFSKALDGDPDCGGLLSYGYHSGENITGVNEGRPLFVRTPDS
                     KFDLANFMRVHLSSAFGAMRIGMEILKKENIKIDKLVGHGGIFKTPEVGQRILAAAME
                     APVTVMETAGEGGAWGIALLASYLTDKNNLTLEEYLSTKVFAKSEGITIEPTNEDIEG
                     YAKFIDRYEKGLSIEKASVVV"
     gene            11524..12222
                     /locus_tag="IXT20_002223"
     CDS             11524..12222
                     /locus_tag="IXT20_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HAQ4860654.1"
                     /translation="MLEQLKEEVFQANLELPKQGLIKYTWGNVSAFDSESGYFVIKPS
                     GVSYEAMKASDMVVVDLDANVIEGKMNPSSDTPTHAILYEKFPEIGGICHTHSTWATI
                     WAQSGLDLPAMGTTHADTFYGTVPCARFLTQEEIENGYEKETGKVIVDTFKKREIKEL
                     EVPGVLLHGHGPFTWGESAKAAVMNAVVLDEVAKMNLFTRQLNSFAEELPQRVLDKHF
                     LRKHGKNAYYGQAK"
     gene            12241..13665
                     /gene="araA"
                     /locus_tag="IXT20_002224"
     CDS             12241..13665
                     /gene="araA"
                     /locus_tag="IXT20_002224"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HAQ4860655.1"
                     /translation="MLEIRKKEFWFVVGSQHLYGEEALKTVKMNAQAIVKGLNDSGKL
                     SYPIVLQDLAVTSDKITEIMKEANYRDEVAGVITWMHTFSPAKMWIRGTKLLQKPLLH
                     LATQFNESIPWETIDMDFMNLNQAAHGDREYGFINARLRKQNKIVVGYWQTSGVQDQI
                     SAWMDVAAAYNESFNIKVARFGDNMRNVAVTEGDKVEAQIQFGWVVDYYGIGDLVQYV
                     DAVTDGEVEKVFAEYKDLYDFDYGDYEKEVWEAHVKVQARQEIGIRRFLEAGNYNAFT
                     SNFEDLYGMKQLPGLAVQRLMAEGYGFAGEGDWKTAALDRLMKVMAHNENTGFMEDYT
                     YELASGKESILQSHMMEVDPTLAVNKPKIVVSPLSMGDREDPARLVFDGKAGAGVVVS
                     MADFGTHYKLLINEVDLFEPTEAAPKLPVARVLWEVKPNFHEGVGAWIEAGGGHHTVV
                     SLNLTTDQIQTWAKMVDLEFVTIK"
BASE COUNT         4557 a         2026 c         2843 g         4274 t
ORIGIN      
        1 ataaaactat ctttttattt taatcatgac gaaaaaggtc aaaggcttag atatattaat
       61 ttggagcaaa aataagatgg ataaactttt cattaatata ctttagagtg aacgaagttt
      121 gaaatataag taaagaagaa aattgagaaa aagattgttt ggctaacggg aaaagtcgtc
      181 ttatcctcag gatcaatctt ttttctccct ccattcacca gtcgaattac cttcgtttac
      241 gaagttgaac atagacaaac gattgaaaga gtggttcttc aaagctagct cccacttttc
      301 agcctaagaa agaattagaa aatttttttg aggaacatcg agatgaatgt tttataaaag
      361 atacttatat gttagcaatt cttttcgttt atctttttct agattctgtg taatcgtttt
      421 agttgtaatt taatacactc tttgttacca tttttatata gtatagttgt aaaaattttg
      481 atgtttaaat atggagtagt gttatttatt ttttagttcg atgcctaaat tttttgaagg
      541 attttgttat atttttatat aaaggatgtg gcggttatga caaaatatat aatggtaatt
      601 tttagtcttg ttgtattaac agcttgttca aagggagaag attcaactgt agtggaatca
      661 acttcaacag attctactac agttgaaaca tcggttacct ctactacaaa gtcaagcact
      721 gaagagatta gcactagtag tacagagaca acacaggatt ttccttatgc cgtaaacttg
      781 aatgatttta ttcaagaagt gaaaccaaat ggaaatgaag cgaataaaaa aactatttcc
      841 catcttactt ttgttacgaa tcaaaaaaat gttcctacta cgattacttt aaatacaaag
      901 gatttaaatg attttggaaa tggaatctat atttcttccg atacagagga ggtattctat
      961 ccagtttcaa ttaatgaaat acctacccaa aatatcattc ttgtcggaga taatggggaa
     1021 gaacgagaag taaaagttaa cactgaagta aaaatcgaag tgccaaacga tgatcagtca
     1081 gattcgttcc aaatagtagg ggatagctat tatctctttt ataatcaaca agaaactatt
     1141 tctttagcaa ctcgaaattt tgatgaaaac ccaggatcga aagatttaga caatagtaat
     1201 atgatagagt atgttcaaca agtggattcg caaaacacag aggactctga accaacagag
     1261 aacacgaaaa gtgatgaata ctatcattct attaaagagg catggaataa acaaaaagat
     1321 tatgtagatg ggattgaaga tcctaaaata aaacaagcta cacagacgcc tttttctgca
     1381 gcaaatgctg aagcaacgag gttagaacaa gaaaatccgg aggatgctga agtaataaaa
     1441 gcaagtctga aaagggttct tgatggggaa taatttaggg gaaaatttct ggatatgacg
     1501 gccatcctga aatcgagctg gcactaggtc gcttatatga attgactcag aaagaaagtt
     1561 atttgacatt agcaaaattc tatattgatg aacgagtaca agacgatttc tttgatcgcc
     1621 aaatcgaagc ggacggattc gaacgtgatg tgatagatgg tatgcgcaat tttccattat
     1681 cttactatca agcggcggaa ccaattcgag aacaagaaac cgcagaaggt catgcagtgc
     1741 gggtcgttta tctttgtaca ggaatggcga aagtcgggag atatacaaat gatgaaagtt
     1801 tattaaaagc atgcaaacgt ttatggaaca atatcgttga acaaaaaatg tacattacgg
     1861 gtggtgttgg ttcaacgaat atcggagaat ctttcacagg aaattatgat cttccgaatg
     1921 atacgatgca tggagaaact tgtgcatccg tcgggatggc cttctttgct aaggagatgc
     1981 tagagctaga aagcaaaggt gaatacgccg atgtcttaga gaaacaatta ttcaacggaa
     2041 tcattagtgg catgtcatta gacggcgcgc actttttcta tgtgaacccg ttgcaggcag
     2101 atccagaagt ttcagagcac aaccccagtc gtaaccatgt gttgacagac cgggcagaat
     2161 ggtttggctg tgcttgctgt ccaagtaatg tggctcgact tgtagcttca gttgatcact
     2221 atatttatac tgtcaaggat acgatgattt tatcccatca gttcatttca aatgatgcga
     2281 ctttcgaaag tggcgtgact attattcaaa cgagtaatta tccgtgggaa ggtcagattt
     2341 attatgagat caacaatcct aatgacgtgg aaacaacctt agggattcgt atcccggcat
     2401 ggtaacaaaa caattttgtg ttgaatatca atgggcaaga taagacaatg aaagtagaaa
     2461 acggctttat ttactttaaa cttctagcag gttcattcga gattttactg acattggata
     2521 tggtagtgaa aaaataccaa gccaaccagc aaatagctca tgattttgga aaagttgcaa
     2581 tccaacgagg tccgattgtt tattgtgtag aatcaatgga ccaaaccgag gatgtgtgga
     2641 agtatcaatt gatccaagca ggagagattg cctatgcttt tgatgacaaa ttgctgaatg
     2701 gtgttggtgt gatccgagca gaggcaaaag tcgcgaatgg acgatctgct agtgtatatc
     2761 aagaatatca accattaact cggcgggcag cggagttgaa gctgattcca tattatgctt
     2821 gggcaaatcg cgggaagaat cagatgttgg tttggatcga ccagaatgat tgctgatagc
     2881 aaaagatagg attaataata taatagactc ggtcggcata cggatttttg agatatctgt
     2941 gtactgaccg agttttagta atacagactt attaatctat gaaaacagtt tggcttcttc
     3001 taagatgtct ttgttcacgg tattctgttg gcgataatcc gtgaaacttt ttaaagattt
     3061 ttgaaaaata agaaggatta ttaaaaccac atactttact aatgtaatca accgaccagt
     3121 tagtagtaaa aagaaactct tcactttgtg aaattctgaa ttcaactaaa tattttttta
     3181 aaggtgttcc aacatgtttt acaaaaatgc tagatagata tgaacgatta atattcaaac
     3241 gatttgcaat atcattgaca gttaattctg taccataaaa atcttcaaca atcataattg
     3301 cctcttgaac atatttttct gcggaatact tttcttcatc aattatcatt tcttttccaa
     3361 attcatcggt accaatcttt gacaacaata ttaaaaattg accatttata tacagatcat
     3421 tgacagtatt attgatgtta ctctttatta tgttttctaa taacttcttt atttcaataa
     3481 atgtatctgg ttcaatatct cttgttaaag ttgggtagcc taatccgaga cgattaataa
     3541 aatggctagc caaacctcca ttaaatccca cccaagaata tgtccatggg gtatcttcat
     3601 cagcttgata aaaaaccatt tgatcaggtt caactaggaa gagttgccca gcttcaattt
     3661 gatattcggt tttgtttaca agtagctttc cttttccaga caatacaaaa tgtagtaaat
     3721 agtatggtct cgatccaggt ccataagaat gtagtggtaa acattcagag tgaccacaaa
     3781 atctaatctt caaatcattc catgctgaat cccgataaag atttaagttc tcaatctcca
     3841 atttaattcc ctccaataca aaaccaacaa aagttttata ctaactaaca tttttatcta
     3901 acagtttgta tatagtctat tatattataa atccagaggc aaggataaat gaatacgaat
     3961 acattagtaa tgctaacgta tggagtgtaa gactagaagt tatatttaat tttcaatttt
     4021 tgtgttattt tatattgatg attatagaac tactattcta acaaaagttt agcttcttaa
     4081 ttactttttg ggcaggttat atttgagaat ctccatttct aaatattgta attgcttatt
     4141 ttaaaaagac tggaaattct gataattatt aatatcaaat gatcgagaga tcatcgatat
     4201 atacacttta tttttgaaag gatgagaaat ttgagaaatt ttgcaaaaac tccacctaag
     4261 ggttggaaca gctgggatgt ttatggagca tctgtaactg aagaggaagt tcggcgcaat
     4321 gcggatgtga tggcgactaa attaaaagat cacggatggg attacgttgt tgttgatatt
     4381 caatggtatg agccaacagc tgattcagct gcctaccatg tgtttgctcc attaaatatt
     4441 gatgaataca gtcgagtaat gcctgcggaa aatagatttc ctagtgccaa agatggaaac
     4501 ggattcaaag atctaggtga ttacattcat agcttaggtt tgaaatttgg tattcatatt
     4561 ttacgtggaa ttcctcgtga ggctgtccat aagcaaatga aaattaaagg aactaccaaa
     4621 acagctgatc aaattgcctt taacagtatc tgtccgtgga actctgatat gtatggagtg
     4681 aatgtaaata ttcccgaagg acaaatgtat tatgactcaa ttcttgagat gtatgctgaa
     4741 tggggtgttg atttcattaa agttgatgat attgcttatt caacaattta tagagaagca
     4801 catcttgcag aaattgctgc tattagaaaa gcaatcgata aaacaggaag agaaattgta
     4861 ctatctttat cacctggacc agctcgtttg caagatggaa gcttcttaca agaaaatgcg
     4921 aatatgtggc gtttaacgga tgatttctgg gatgattggg aacttctata tgacatgttt
     4981 gatcgttgca attattggtc tccatttatc aggccaggaa attggcctga ttgcgacatg
     5041 cttccactgg gtcacatcgg gatacgttct ggtgagcgtg gttttcctga tagaaatacc
     5101 aactttacaa aaccagagca aaaaacaatg atcacacttt ggtccatttt ccaatcacca
     5161 ttaatgtacg gtggggagtt agctgactta gacgattgga cattaagtct ccttacaaat
     5221 gaagaaatta attcaatgca ttcttcactt accaagcaaa aacaagaatc tagaaatgaa
     5281 aactggattg tatggtcagg tgaaaacgaa aaagataagt acattgctat ctttaatgtt
     5341 tcgaataacg agcaagatat tccagaagaa attctgaacg aatatattca gacagaaaat
     5401 gctaaagagc tatggtctgg tgattcaaag gatgctttta ataaaactct tgaaagccat
     5461 gattgcgcta ttttcaaatt ataaaaataa tttgaacaaa aaaataaaat aaacataggc
     5521 ggaaagctga tttcaccctt aactctatca gctttccgca ctaaagatga tggaatcgaa
     5581 aataaaaatg aacttattca ttttatagtt atagatcggg tttatcacta gaataaggaa
     5641 ggttttgaaa aaaatggata ctattggaaa aagcctaggg ctaaaagaaa aaatggctta
     5701 tggttgtgga gatgcagcga gtaatatcat atgggcatct atgggcgctt tcatgatgtt
     5761 ttattacact gatgttgtaa atatcagtgc agctacaatt gggacaattt ttttgatttc
     5821 acgggtcata aatgcagtag ttgctttggc gacaggttgg ttgattgata atacaaaatc
     5881 gaaatatggg aaagccagac cttggttatt atggtttgca gtaccatttg cgattgtaac
     5941 gtttgctatc ttttcagtgc cagatattgc aaaactatat caagtaatat atgtatttat
     6001 cacctataat gcaatgatga cggtctattc attaataaat attccatatg gatctatggc
     6061 aacaatgatg acaaaagatc agtatcagag aacattgcta aatatttttc gtcaattatt
     6121 tgcccaaatt gccagtttat tggtaacagc atttacttta ccgttagtga aagtttttag
     6181 ttcttcagta ggtgagaaga atgcttggag tgttgtatat gggatttttg gtattgcagc
     6241 aggagtacta ttcttcttgt gttttattgg aaccaaagaa cgcattcacg ataacgaagt
     6301 agttaaggaa gaaaaaatca atattgttaa gggaatgaaa tcagttctaa aaaacaatta
     6361 ttgggttatg ctaatggtaa tctcgattag tattaatata tttttccaag caattagtac
     6421 tgtgaacacg tattattgta aagctgtatt gcacgatagt aatatgattg ggattttaaa
     6481 tactgcttat atcgtaccag cgattgtggc attctttttt gtacatttga ttgtgaaaaa
     6541 attcggtaaa ggctatacta caatgtttgg ctggattatt atttgtgcta gctatgcagt
     6601 attattaccc tttactgaaa atcatatggc tttgattatt acatctgcta tgcgtggagt
     6661 aggttattgc tttttattag ctgtttcatc agcaatggtt agtgatgctg ttgagtatgg
     6721 ggaatggaaa acacatgtac gtgtagaggg actaactttt agtatgcaag gatttgtcac
     6781 aacgattgct gctggtttag ttacagcttt tattggtgga gttcttgact ttagtaaata
     6841 tgacggaact ttaacttttg cacaagctca gccagtaacc gctgtaacag ccataaaact
     6901 attatttatt gcaatgccat ttattgtagg tgctttaaat gtattgcttc taaaattcta
     6961 caagcttgat aaaatgtatc ctaagattat tgaggatttg aatatgagaa atagcgtaac
     7021 aactaaataa tagcagttta cgatagcgac cagattacta ttgaattttg caggtagatt
     7081 aagaaatgag tacctatctg caaaattcga tacatttttg tatagtaaag aaagcgtttt
     7141 ataaaggagg attcccagtg aaatctataa tttcagatga aaaagaatgt tcaaaggaag
     7201 aaaagctctc aagtgggttt aaaacttcca ttacagaaaa gattgcgtat ggtatggggg
     7261 atgtcgcttg taatgtagta tacgcattaa cgtctggttt gctggtctat ttttatacaa
     7321 atgtaatagg ggttccggca ggagtgatcg gtacaatatt attactttca cgaatttttg
     7381 acggaatctc tgatttactg attgctcaat tgatggacaa aattaactcg aagcatggta
     7441 aagctcgagc atgggttcta tggatgtcaa ttccatatgc gctgactgct gttgcacttt
     7501 tccttgttcc agcaaatgca acgttaatgg tacagacagt ctatattttt attacgtata
     7561 atctttgtac tactgtagta tacactggat tgaatttgcc ttattcagct atggcaccat
     7621 tgatgactaa cgatgatgat gatttagcta aattgaattt atttagaatg tccatgtcgc
     7681 caatcagcaa tctgattgtt acggccgtat ccttaccttt tattaatatg ttgggtggcg
     7741 atcaaagagc ctggatcatt gttactgctg tatatggggt tgttgctttt ggaatgttaa
     7801 tatggacatt tttaggtaca aaagaaagat ttcacacatc tgctgctgct gaggctgagg
     7861 agttaccatt ctctcagcga ctgaaagcta ctggaaaaaa taagtatttt ataattatgt
     7921 tcttgaccgt gatatgttta tcaatttatc aaaacgttaa tgggacagtt tctacttatt
     7981 atgctcaata cattctaggg aacgctgaat tgatgggatt aatgcaaaca tttgagaaag
     8041 ttccttggat acttggaatt atgttcatgg ctccactaat acgacgcttt ggtaaaagaa
     8101 atttagtttt gtttggagcg attttatgcg ttggagccca agtgttaatc atgttcaatc
     8161 cgagcaattt aaacttgata ctcttttctg ctgtactaag aggattggga gaagcaccaa
     8221 ttaatggttt aacatttacc atgattgctg acgtaataaa ctatggtcat tggaaaacag
     8281 gaatccgagt ccacgccctg attttttcaa cttttacagt tggtcaaaag tttggtggtg
     8341 gaattactgg atgggcaatt ggacaactat tagaagtctc tggtttttcc ggttctgtga
     8401 ctgaaatacc ttctgccgta tctatggttc aaaaccttta tatttatgga acgatccttg
     8461 cttgggcttt ggtagcagtc tgtatgttga aatatcattt agataaagaa tatgatgaaa
     8521 ttatggtcga tttaaagaat agagaaggag ataaactaac tgtttaacaa tcacttagga
     8581 taaagagttt ccactttaaa tagcgggaac tcttttattt atgtatggta taataaaaga
     8641 gtcagtcgaa tttgttcgta catatttatt gtgaggtgga gaaatgtcta aatcaaaata
     8701 tcaaattatt gcagaagaaa taaaggggaa aatcgaatca aacgagtatg ggtttaatca
     8761 gactattcct ccagaaatgc aactgcagaa agattatgca gttagtcgtc ataccgtgcg
     8821 gcaagcaatt gcagtcttag taaatgaagg ttatctaaga aaagaaaaag ggtcgggcac
     8881 ttatgttgat aatcggttca aaaatgattc gaaagtaaaa gcgaccccta agacaattgg
     8941 agttatcact acttatttgt cagactatat tttcccatca atcatcaggg ggattgaggg
     9001 aaccttgaga agtaaaggtt actcactctt acttgctagt acaaataatg actatcagca
     9061 ggaaaaagag tgtttagaaa aaatgattaa ttatggtgtt caggggttga ttgttgaacc
     9121 tacaaaaagt aaccaataca atccaaatct ggctttgtac gtgagtttaa aggaacaagg
     9181 aatacctatg gttatgatta atgcagtata tgaagaatta gaggcagctt acatatgtgt
     9241 caacgatgta cgttcaggct ttttggctac tgaattaatg ataaaaaaag gaaataagaa
     9301 tctcttatta gtttctaaaa ttgacgactt gcaaggtaaa tatcgaatga aaggttttat
     9361 tcaagcatgt gaaaagtatg gggtgaaaat ccaatcacag gatataatta catatactac
     9421 tgatacaaga aaacaggttt cttcaattgt tttgaaacgt ctaaaagagg aaaaaaaaat
     9481 caatggtatt gtctgttaca atgatcaaat cgctagcgtc attgtagaca atttgaattc
     9541 acaagagata agtgttcctg atgatatatc cattattgga aacgatgact cttccttaag
     9601 caaagtcggt agtataaaaa tgactacatt gaaacacccc aaagttgaga tgggaaaaga
     9661 tgctgctgaa tggataataa aaacaattga gaaaaattct gaacaaaaaa gtattcttta
     9721 tgaaccaatc ttaatagaga gagattcggt tcttaatcga tagaataatt tgatgaatag
     9781 acctccaggg aaggtctatt tttttatatg tgtacgaaca aatttcgtcg aaattagatt
     9841 gacgtgtacg tacataaaat gtataattta ggtgttgatt aggataaaag gaggagatta
     9901 ttatcaattc agtaatgact aaagctgata ttatgctaga tattattgat ggccgtacat
     9961 ctttgggaat tgaatttggc tcaacacgca tcaaagcagt tctcgttgct tctgacctat
    10021 cgccaattgc tgctggaagc tatgaatggg aaaatcagtt ggaaaatggt atttggacat
    10081 actcacttga cgatgtttgg aagggcttac ggataagtta tcgtaaaatg gctgctgaag
    10141 tttttgagga gtacggagaa acattgtcta caataggctc tctgggattt agtgcaatga
    10201 tgcatgggta tatggctttt gatgctgaaa acgacttatt agtccccttt agaacttggc
    10261 gcaatgccat tactgaagag gctgaagaaa agttaacaaa agcatttcga tttaatattc
    10321 cacagagatg gagtattgca cacttttatc aagctatttt gaacaaagag aagcatgtcg
    10381 gaaatgtaac cttctttact acattagccg gatatatcca ttggcaatta tctggtgaaa
    10441 aagttcttgg tgtgggagac gcatctggaa tgttcccaat tgacgttgca attaaagatt
    10501 atgaccagca aaaaattgct attttcaata acttggttaa aaaagaggat atggatataa
    10561 atatcaaatc atttttacct agagttttat tggctggaga agctgctgga aatttaacac
    10621 aagagggggc gtatcttctt gatccgacag gtaccttgaa agcgggtatt ccattgtgtc
    10681 caccagaagg agatgctgga acgggaatgg tagcaacaaa cagcgttgct aagcggacgg
    10741 gaaatgtgtc tgcaggaact tctgcctttg caatgattgt tttagaagag gatttgaaag
    10801 aagttcatcc agaaattgat ttagtaacaa cgccttcagg tgaacatgtt gcaatggttc
    10861 atacaaataa ctgttcttct gaaattaatt cttggataaa aatatttaaa gaatttactg
    10921 aaacactcgg atttgagatt agtacagaat cgctttatga caagctattt tctaaggctt
    10981 tggatggtga tccggactgt ggtggtttat tatcttatgg ataccattca ggagaaaata
    11041 tcacgggggt aaatgaaggt cgtccattat ttgtacgtac gccggatagt aagttcgatt
    11101 tggcaaattt catgagagtt catctttcta gtgcattcgg agctatgcgc ataggtatgg
    11161 agatattaaa aaaagagaat attaagattg ataaattggt tggtcatggt gggattttta
    11221 aaacaccaga agttggacaa aggattctgg ctgcggcaat ggaggctcct gttaccgtaa
    11281 tggaaactgc tggagaagga ggagcttggg gaattgcttt attagcttcg tacttaactg
    11341 ataaaaataa tttaactttg gaagaatatc tttcaactaa agtttttgct aaaagcgaag
    11401 gtattacaat cgagccaaca aatgaagata ttgaaggata tgctaagttt attgatcgtt
    11461 atgaaaaagg cttatccatt gaaaaagctt cggttgtcgt ttaaaaaaaa taggaggtta
    11521 ggtatgttag agcaattgaa agaagaggta tttcaagcga atctggaatt accaaaacag
    11581 ggattaatta aatatacatg gggaaatgta agtgcctttg attcagagag tggctatttt
    11641 gtcattaaac caagtggtgt aagttatgaa gcgatgaaag cttccgatat ggttgttgtg
    11701 gatttagatg ccaatgtaat tgaagggaag atgaatcctt cttccgatac gcctactcat
    11761 gctattttat atgagaaatt tcctgaaatt ggtgggattt gtcatactca ttcaacttgg
    11821 gcaacaatat gggcacaatc gggtttggat ttacctgcaa tgggaacgac tcatgctgat
    11881 acattttatg gaacggtacc ctgtgcgaga tttttgacac aagaagagat tgagaacggc
    11941 tatgagaaag aaacaggaaa agtcatagtt gacactttta agaagcgtga aatcaaagaa
    12001 cttgaagtac caggcgtttt attacatgga catggaccct tcacatgggg agaaagtgcg
    12061 aaagcagcgg taatgaatgc agttgttttg gatgaggtcg ctaaaatgaa tctttttact
    12121 cgtcaactga actcatttgc agaagagtta cctcagcgtg tattagataa acatttttta
    12181 agaaaacatg gtaaaaacgc ttattatggt caagctaaat aaagaaaaga gggaaaattt
    12241 atgttagaaa ttagaaaaaa agaattttgg tttgtggtag gatcacaaca tttatatggt
    12301 gaagaagctt taaaaacagt aaaaatgaat gcccaagcaa tagtaaaagg attaaatgat
    12361 agcggtaaac tatcgtatcc gattgttttg caagatctag ctgtgacttc agataaaata
    12421 actgaaatta tgaaagaagc caattaccgg gacgaagttg caggtgtaat tacgtggatg
    12481 cacacctttt ctccagcaaa aatgtggatt cggggaacaa aattacttca aaaaccttta
    12541 cttcatttag cgacacaatt caatgagagt attccttggg aaacaattga tatggacttc
    12601 atgaatttga accaagcggc tcacggagat cgagaatatg gatttatcaa tgctcgcttg
    12661 agaaaacaaa ataaaattgt tgtaggatat tggcaaacat ctggagtaca agatcaaatt
    12721 tctgcttgga tggatgttgc cgcggcttat aacgaaagtt ttaatatcaa agtagcccgt
    12781 tttggagata atatgcgaaa cgttgctgtt actgaaggcg ataaagttga agcacaaatt
    12841 caatttggat gggtcgttga ttactatggt attggtgatt tagttcaata tgtagatgca
    12901 gtaaccgatg gggaagttga aaaagtattt gctgagtaca aagatttgta tgactttgat
    12961 tatggtgact atgaaaaaga agtttgggaa gcgcatgtaa aagttcaagc tcgacaagaa
    13021 attggaatta gaagattttt agaagcaggg aattacaatg cctttacttc aaattttgaa
    13081 gatttatatg gtatgaaaca actccctggc ctagctgttc aacgattaat ggcagaaggg
    13141 tatggttttg ctggtgaggg cgattggaaa acagctgcac ttgatcggtt gatgaaagtc
    13201 atggcacata atgaaaatac tggtttcatg gaagattaca catatgaatt agcatcgggt
    13261 aaggaatcta ttctgcaatc tcacatgatg gaagttgacc ctacacttgc tgttaataaa
    13321 cctaaaattg ttgtttctcc attatcgatg ggtgatcgtg aagatccagc gcgattagtt
    13381 ttcgatggaa aagcaggagc gggtgttgtg gtatccatgg ccgattttgg gacacactac
    13441 aaattattaa ttaatgaagt agacctgttt gagccaacag aagcggcacc taaattaccg
    13501 gtagctcgtg tactttggga agttaagcct aatttccatg aaggcgttgg agcttggatt
    13561 gaagcaggcg gaggtcacca tacggttgta tctttgaatc tgactactga ccaaattcaa
    13621 acttgggcca aaatggttga tttagagttc gtaactatta aatagatcct aagaattggt
    13681 ttgggaaacc aggccaattc
//
LOCUS       DACGLE010000072        13456 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0534
            SAMEA3304449-rid9560453.denovo.076, whole genome shotgun sequence.
ACCESSION   DACGLE010000072 DACGLE010000000
VERSION     DACGLE010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304449
            Sequence Read Archive: ERR985064
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13456)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13456)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702225.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702225.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 20:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,045
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 27 of 67
            Pseudo Genes (incomplete)         :: 25 of 67
            Pseudo Genes (internal stop)      :: 19 of 67
            Pseudo Genes (multiple problems)  :: 4 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13456
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304449-rid9560453.denovo.076"
                     /strain="VREN0534"
                     /db_xref="taxon:1352"
     gene            complement(<1..924)
                     /locus_tag="IXT20_002225"
     CDS             complement(<1..924)
                     /locus_tag="IXT20_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HAQ4860656.1"
                     /translation="MRRFAINGYGEAQDVFEEIDAHPREVGSGHVRVEIKAFSVNPYD
                     VALRLGEMKEIRTLKFPYVPGNDGAGIVTEIGSDVTTVHVGDRVAVHAVGGTYGEEVV
                     LPSAKVAKIPDKMSWEEAAGMVTPGITAYNLINHLTEIQPTDTVMILGASGAVGSSLI
                     QLLHEKGIRILTSASSKNEEKVKKLGASAFAAYDKTNPGLQFADQADLVIDATKGSIK
                     GETGIQIMKPGGRYVALNDLPDLDLRQKKEGFYESFVPRKEYLDAEAFAGIIKAYQKG
                     AFHVFISMNLSASLKHVIQAHQLVEGHPPAGK"
     gene            1114..1383
                     /gene="rpsN"
                     /locus_tag="IXT20_002226"
     CDS             1114..1383
                     /gene="rpsN"
                     /locus_tag="IXT20_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HAQ4860657.1"
                     /translation="MAKKSKIAKAKKQQETIERYAALRQELKAQKDYEGLRKLPLDAR
                     PERLKNRDLIDGRPRGYMRKFGMSRIKFRELAHKGLIPGVKKASW"
     gene            complement(1559..1921)
                     /locus_tag="IXT20_002227"
     CDS             complement(1559..1921)
                     /locus_tag="IXT20_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HAQ4860658.1"
                     /translation="MVKRGDIYFADLSPVVGSEQGGTRPVLVIQNNLGNHFSPTIIVA
                     AITAKMAKPKLPTHIGIKATGTGIERDSVILLEQIRTIDKSRLKEKVCHLDRPIMAEV
                     DRALKISVGIEALTPVTK"
     gene            complement(1932..3053)
                     /locus_tag="IXT20_002228"
     CDS             complement(1932..3053)
                     /locus_tag="IXT20_002228"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HAQ4860659.1"
                     /translation="MVVAWHRPTKAVIHKKAITENVANEVARLPQGKELFAVVKANGY
                     GHGAIETAEAAVAGGASGFCVSNLDEGVELREAGFTQPILILNMVPYDALTVAVAHDL
                     SVTAGTREWLQAAAAVLEKSKLETPLSIHLKADTGMGRIGFCTPEEVKEAAAFIKESR
                     VLEWEGLFTHFSTADQADDTYWNLQKERFIEVLKKLPELPRYVHVSNSATALWHDETI
                     GNMIRYGVAMYGLNPSGHALPEVYPLQPALELVSELIQVKKLPAGEGIGYGETYITPE
                     AEWIGTIPIGYADGWPRKMQGFSLLVEGNYCETIGRVCMDQLMIRLPQEFPVGTKVTL
                     IGKNADKEITMQDIADQLGTIHYEVACGLGQRIPREYQE"
     gene            complement(3069..3419)
                     /locus_tag="IXT20_002229"
     CDS             complement(3069..3419)
                     /locus_tag="IXT20_002229"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HAQ4860660.1"
                     /translation="MIKGIGIDAVELPRITRLIEEKPKFIARILTSDEMKLFQSLPFH
                     RQVEFLGGRYACKEAFSKAWGTGIGKVTFQDVEILKNENGQPVVTRSPHEGNVWVSIT
                     HTNETAFAQIILED"
     gene            complement(3549..5060)
                     /locus_tag="IXT20_002230"
     CDS             complement(3549..5060)
                     /locus_tag="IXT20_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HAQ4860661.1"
                     /translation="MKFKELELSPELLKSVERAGFEEATPIQAETIPLALAGKDVIGQ
                     AQTGTGKTAAFGLPMLEKIDPDRHELQGLVIAPTRELAIQTQEELYRLGRDKKVRVQA
                     VYGGADIGRQIRGLKDRPHIVVGTPGRMLDHINRHTLKLGTVQTLVLDEADEMLNMGF
                     LEDIEKIISQVPDQRQTLLFSATMPPAIKNIGVKFMKNPHHVKIKAKEMTADLIDQYY
                     VRAKEYEKFDIMTRLFDVQTPELTIVFGRTKRRVDELARGLEARGYRAEGIHGDLSQQ
                     KRMSVLRSFKSGHLDILVATDVAARGLDISGVTHVYNYDIPQDPESYVHRIGRTGRAG
                     KGGMSVTFVTPNEMDYLHVIENLTKKRMTTLRPPTEKEAFKGQLGAAVEQIETKLAEN
                     GLDKYLQTADKLLEEYSAQDLVALLLKTTAKDPADAVPVKITPERPLPMQKKGYNKNG
                     KRGGGNNRNRRDGGNYRGNKSKGGYSKNNNSQKDGGKRHNDKKRGFVIRSNND"
     gene            complement(5382..6734)
                     /locus_tag="IXT20_002231"
     CDS             complement(5382..6734)
                     /locus_tag="IXT20_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HAQ4860662.1"
                     /translation="MNLTIQEIAQAVGSECSALTKITNVEFDSRKITEGSLFVPLAGT
                     RDGHEFIQQAITNGASASFWSLPVEQAPKNFPVIPVADPLKAMQQLATYYLEKVGAEV
                     VAITGSNGKTTTKDLTASVLSEKYKTYKTQGNYNNNIGLPYTILHMPQDTEKIVLEMG
                     MDHANEITELSLMAQPKAAAITMIGEAHIENLGSREGIAKAKMEITDGLVSDGLLVVP
                     ADEPLLTPLTATLVQTVTTFGIGNGDVHAEVTAKGKAQTDFVVEGETFTIPLPGSYNV
                     TNALIAYTIGRFFGLSIPEIRQGLAHVSITQNRTEWLTAGNGAAILSDVYNANPTAMG
                     LVLDTFKNLPTKGRRIAVLADMLELGKESLNMHAQMSEHIDPEKFAMIFLYGEQMHAL
                     KEELNQKYPQLPVFYFEKEKNKLIDAIKQNLAATDSIMLKGSNSMGLFEVVEQLQQMK
                     "
     gene            complement(6887..7963)
                     /locus_tag="IXT20_002232"
     CDS             complement(6887..7963)
                     /locus_tag="IXT20_002232"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HAQ4860663.1"
                     /translation="MKITLLYGGRSAEHEVSILSAFSVLNAIYYNYYQVQLVFITKEG
                     QWVKGPLLTEKPASKDVLHLSWDPSGQTEEGFTGKVINPGEIKEEGAIVFPVLHGPNG
                     EDGTIQGFLETLNMPYVGAGVLTSACAMDKIMTKYILQAAGVPQVPYVPVLKNQWKEN
                     PKKVFDQCEGSLLYPMFVKPANMGSSVGITKAENREELQNALATAYQYDSRAIVEQGI
                     EAREIEVAVLGNEDVRTTLPGEVVKDVAFYDYEAKYINNKIEMQIPAEVPEEVYQKAQ
                     EYAKLAYTMLGGSGLSRCDFFLTNKNELFLNELNSMPGFTEFSMYPLLWENMGLKYGD
                     LIEELIQLGMNRYHQRQSFFEKNE"
     gene            8159..9904
                     /locus_tag="IXT20_002233"
     CDS             8159..9904
                     /locus_tag="IXT20_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/ABC1/UbiB kinase family protein"
                     /protein_id="HAQ4860664.1"
                     /translation="MAQTNPRSRLAEIIRVFINYNVVPNFVQQKNPEQVKKAFEELGP
                     TFIKIGQMLSVREDLLSSAFTQTFKTLQDSVPSDTFSTVKKTIETELSLSLSDIFDDF
                     SKSPFASASMGQAHRAKLKNGDAVVVKIQHPNIAEEIRLDLQLFERAIPLIKYIPETS
                     VVDLKGVLQEVKRSLINEMDFLKESQNGEQFYQKNNGWKEIRSPKIYDAFCSKKVIVM
                     EEMSGKNLNHLMNAENKTETFITGIQNKQLKQEVAKLLVENFMKQVFDDGFFHADPHP
                     GNLLFHVLTKEEQTQASRKTETVHEKEFGSFAFRASTSAEDPVAPYTINYIDFGMMGH
                     LSAGLRQKLTQAVLALYTKDAYRIEKAVLRLCQQEGSFDESRFHQELTSFLEQYYDSP
                     IDEINLQEVFTHVVTICHQNNLQFDRDITLLLKAFGTLEGVIRVLDPEVSLMEVASPF
                     AQHYFLTHLDVEDTLKQSGLDLLEGMKAAPKIPQQLHHLLEMWTSGQGKVNLELKKQD
                     KLLSRIESMINRLVFGMILAALIVGSSLLVQAAPVENAEVVSLLGIFTYAIAAFVIIF
                     LAIDALIQMYKKRKK"
     gene            complement(10184..10621)
                     /locus_tag="IXT20_002234"
     CDS             complement(10184..10621)
                     /locus_tag="IXT20_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HAQ4860665.1"
                     /translation="MRLFIDGDGSPVKEDVIKLGESYGLDVVIVTSVDHYTTKEYANH
                     VQIVYVDRGADSADYKIVGMVKKNDFLITQDYGLASLVLPKGVRVFHHSGKEFDEANI
                     GTLLEQRYHSGKLRKAGIRTKGPKPFTEKDHMSFRQALKKAID"
     gene            complement(10605..11978)
                     /locus_tag="IXT20_002235"
     CDS             complement(10605..11978)
                     /locus_tag="IXT20_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4860666.1"
                     /translation="MFEKLKRFGRNFTQTTPFILSNTVIFIPYLLFLGLTDSHNWLTI
                     LPFTLFYAFRMTGLFLVNSLRFGLDSYTLLMIALLIGGAGSLLGILGVFYFPLFYFSS
                     VLLGLSAAWLVPANTTVNFHEKTQGFINMDGKKYLFAAIWLFLLYQAIELPAPTQIAA
                     SFTIYTLFYVMAYHTVSHYPRYELDFKDIKRNLVATRELVLFLIFFGLLFLLRNARLL
                     FDERLFDLAIYGFLLLFILFVFVLGHQKKEWKLPSWLNLFTFLNGMLGNFLFLFSSFY
                     VGILYGLEKLSIFMYLPYAAGLIAAKIVGPWILKRLTWSPLAMQLLGLSSSLLVLLIP
                     NGFAIGVFLLSFWHVVTGSWLNKEYYQTDTAIPMDRRIVVKYTTQNKGSVIHQFLLMT
                     LLFVLAKEMGEPIHLLLITLNHESVPIESVQLLILAKWINVGLLLSGLVTVYHLWKKA
                     KKNEAIH"
     gene            complement(12300..12788)
                     /locus_tag="IXT20_002236"
     CDS             complement(12300..12788)
                     /locus_tag="IXT20_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ4860667.1"
                     /translation="MEKIKVTENELDELMAVIQEVWPEAFVPIIGQKQVDYMLKTYQS
                     KKQIQKELTEGVSYFLLKSEEKTVGYTAYEEINGKIYLSKLYLLNKVRGQGLTSSVFR
                     WYEELAAQTKEREIFLRVNQGNHQAIEVYKHEGFEITEELISDIGQGFQMVDYKMKKA
                     LA"
     gene            complement(12810..13412)
                     /locus_tag="IXT20_002237"
     CDS             complement(12810..13412)
                     /locus_tag="IXT20_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HAQ4860668.1"
                     /translation="MSKKGSVLQKCLQIMPIIGLFFFFVLILYAYHHGIFRSSTSLQA
                     FIQQFGKYAVMIFVLLQVIQVIIPILPGGISSVAGMLMFGNGFGLLYSCIGLVIGEAI
                     GFLLVRYYGVAFVQLILSPKKYQKFDELLTRKTKDIKKVLVVTLLLPFAPDDLICLVA
                     GLTKLSFREFMQIVIFLKPWSVGVYSMIMLFLFHQAQGHL"
BASE COUNT         3853 a         2923 c         2446 g         4234 t
ORIGIN      
        1 tttcccagca ggaggatgtc cttcaactag ctgatgtgct tggatgacat gcttaagtga
       61 agcagacaga ttcattgaaa taaaaacatg aaaggctcct ttttggtagg ctttgattat
      121 gccagcaaag gcttctgcat ctagatattc ctttcttgga acaaacgatt cataaaagcc
      181 ttcctttttt tgccgcaaat ctagatctgg gaggtcattt aatgcaacat acctgccgcc
      241 tggtttcatg atctggatgc cggtttctcc tttgatactt cctttcgtcg catcgattac
      301 aaggtctgcc tgatcagcaa attgcagacc tggatttgtt ttatcataag cagcaaatgc
      361 tgaagctcct aattttttta ccttttcctc atttttactg gatgcactgg ttaagattcg
      421 gattcccttt tcatggagaa gctggatcag acttgacccg accgcacctg atgctcctag
      481 tatcattaca gtatctgtcg gctggatttc cgtcaaatgg ttgattagat tataagccgt
      541 aattcctggc gtgaccattc cggctgcttc ttcccaactc attttatctg gaatttttgc
      601 tacttttgct gatggcaaca cgacttcttc tccgtaagtc ccgccgactg catggacagc
      661 tactcgatca cctacatgaa cagttgtgac atctgaacca atctctgtca caataccagc
      721 gccatcatta cctggtacat aagggaattt caatgttcgt atctctttca tttctcctaa
      781 acgcaaggca acatcataag gattcacact gaatgctttt atttctacac gaacatgtcc
      841 agaaccgact tctctgggat gagcatcgat ttcttcaaaa acatcttgtg cttctccgta
      901 accgttaatt gcaaatcttc gcatggtcac catctccctt tttctttaag tttacgttta
      961 tgtctttatt ttgacaaaaa aaatgtcttt tcgttttggc gcatgattca aaacaaaata
     1021 gttgtctttt ttcgcgtaac gtgttaaatt atttatctaa aagcgtaaag attacgattt
     1081 acgctaataa aaaataattg gaggaatttg ttgatggcta aaaaatcaaa gattgccaaa
     1141 gcaaaaaaac agcaagaaac gattgaacgc tatgctgctt tacgccagga attaaaagcg
     1201 caaaaagatt acgaaggctt gcgtaaactt cctttagatg ctcgtccaga acgattgaaa
     1261 aatcgtgatt taatcgatgg acgccctcgc ggatacatgc gtaaattcgg gatgtcccgt
     1321 ataaaattcc gtgaattagc acataaaggt ttgattccag gagtaaaaaa agccagctgg
     1381 taatccaagt ctggctgtaa tgtaaaggag gagggcgcat ggaccactcc ttggatcatc
     1441 aaaaaagata cgttcgcttc ttctagcaga aatgctggta ctagaagcag acgtatcttt
     1501 ttttacgcac agctggtcct tttttatatg taaaggctgc atggttactt cgctatattt
     1561 atttagtaac aggagtcagt gcttcgatac ccacactaat tttcaatgct cggtcaactt
     1621 ctgccataat gggtcgatcc aaatggcaga ccttttcttt caatcttgat ttatcgatcg
     1681 ttctgatctg ctccaacagg ataacggagt ctctttctat tcctgttcca gtagctttga
     1741 tcccaatgtg agtcggaagc ttaggtttag ccatttttgc cgttatggcc gcaacaatga
     1801 tcgtcgggct aaaatggtta cctagattat tttgaataac caaaactggg cgtgttccgc
     1861 cttgctccga tcccacgact ggagaaagat cagcaaaata aatatctcct ctttttacca
     1921 taggatcctc cttattcttg atattctctt ggtatccgct gtcctaatcc gcaagccact
     1981 tcatagtgga tcgtacctaa ttgatctgct atatcctgca tagtgatttc tttgtctgca
     2041 tttttgccga taagcgtcac ttttgttccg acaggaaatt cttgaggcaa tcggatcatc
     2101 agttgatcca tacatacacg cccaatcgtt tcacagtagt tcccttctac caatagagaa
     2161 aaaccctgca tctttcgtgg ccagccatct gcataaccga tcggtatcgt tccgatccac
     2221 tctgcttcag gagtgatata tgtttctcca tagccgattc cttcacctgc tgggagtttt
     2281 ttgacttgaa tcaattcaga aaccagctca agtgctggct gcaatggata aacttccggt
     2341 agtgcgtgcc cagagggatt aagtccgtac attgctactc cataacgaat catattgccg
     2401 attgtttcat catgccataa agccgttgca ctattgctga catgtacata tcgaggaagt
     2461 tcgggaagtt ttttcagcac ttctataaag cgttcttttt gcagattcca atatgtatca
     2521 tctgcctgat ctgctgtaga aaaatgggta aataaacctt cccattctaa tacacgactt
     2581 tctttgatga atgcagctgc ctcttttact tcttcaggtg tacaaaatcc gatacgaccc
     2641 atcccagtgt ctgcttttag atgaatcgaa agaggcgtct ctagcttact tttctctaaa
     2701 acagcagctg ctgcttgcaa ccattcccgt gtacctgcag ttactgataa atcatgagca
     2761 acagctacag tcagtgcgtc gtaaggaacc atattcaaga taaggatggg ctgagtaaat
     2821 ccagcttctc tcaactcgac tccttcatct aaatttgaaa cacaaaagcc cgaagctcca
     2881 cctgcaactg ctgcctcagc tgtttcaata gctccatgac cgtatccgtt cgcttttact
     2941 actgcaaaca attcttttcc ttgcggaagt cttgcaactt catttgctac attttcagtt
     3001 atagcttttt tatgtatcac tgctttcgtt gggcggtgcc aagcgacaac catcgttttt
     3061 ccttctttct agtcttccaa aataatttgt gcaaaagctg tttcatttgt atgagtaata
     3121 ctgacccata cattgccttc atgaggtgaa cgcgtcacaa caggctgacc attttcattt
     3181 tttaaaatct cgacatcttg aaaggtaact tttccgatcc cagttcccca agctttagag
     3241 aaagcctctt tgcaagcgta acgcccgcct aaaaattcta cttggcgatg aaaaggcaag
     3301 gactggaata gtttcatttc atcagaggtc aagattcgtg caatgaattt tggtttttct
     3361 tcgatcagcc gagtgatcct aggaagttcg actgcatcga tcccaattcc ttttatcata
     3421 tgcttttctc ttttcaataa atttagaaca ctctcattct accaaattat aaccattttg
     3481 ccacaaataa gtccacaaat aacaaaaatg aaagtttctc ttaaaaaaga gaaactttca
     3541 ttttatgttt aatcgttatt tgaacggatt acaaatccgc gttttttatc attgtgacgt
     3601 ttaccgccat ctttttggct gttattattt ttgctataac cgccttttga tttgtttcca
     3661 cgataattac cgccgtcacg acgattacgg ttgtttccgc caccacgttt gccattttta
     3721 ttgtatcctt ttttctgcat tggcaatggg cgttctggtg tgatcttcac tggcactgca
     3781 tcagctggat cttttgctgt cgtttttagc aaaagtgcaa ctaaatcttg tgcagaatat
     3841 tcttctaata atttatctgc tgtttgcagg tatttatcta gaccattttc tgcaagtttt
     3901 gtttcgattt gttcaactgc agcacctaat tgacctttga atgcttcttt ttcggttggt
     3961 ggacgtaaag tcgtcatccg ttttttcgtc aagttttcaa tcacatgcaa gtagtccatt
     4021 tcatttggtg taacgaaagt cacagacata ccgccttttc cggcacggcc tgtacgtccg
     4081 atacggtgaa cgtagctttc tggatcttga gggatatcat agttgtacac atgagtgaca
     4141 cctgaaatat ctaatccacg tgcagcaacg tctgtcgcta caaggatatc taaatgaccg
     4201 ctcttaaatg aacgcaatac gctcattcgt ttttgttgag acaaatcgcc atggattcct
     4261 tctgcacggt aaccgcgtgc ttctaatccg cgtgccaatt cgtctacacg gcgttttgta
     4321 cgtccgaaga cgattgttaa ttcaggtgtt tgaacatcaa acaagcgagt catgatatcg
     4381 aatttttcgt attcttttgc gcggacatag tattgatcga tcaagtcagc tgtcatttct
     4441 ttcgctttga tcttcacatg atgtggattt ttcatgaatt taacgccaat attcttgata
     4501 gctggcggca tcgttgctga gaaaagtaat gtttgtcttt gatcaggaac ttgtgaaatg
     4561 attttttcga tatcttccaa gaagcccata ttcaacattt catctgcttc atccaaaact
     4621 aatgtttgaa cggttccaag tttcaatgtg tggcggttaa tgtggtcaag catacgtcct
     4681 ggtgtaccca caacgatatg tggacgatct ttcaagccac ggatctgacg gccaatatct
     4741 gctccgccgt aaacggcttg tacacgtact tttttgtcgc gccctaaacg atataattct
     4801 tcttgtgttt ggatagctaa ttcacgtgtt ggtgcgataa ctaaaccttg taattcgtga
     4861 cgatctggat cgattttttc taacattggt aatccaaatg cagctgtttt ccctgtacct
     4921 gtctgtgctt gtccaataac gtcttttcca gctaatgcta gtggaatcgt ttctgcttgg
     4981 ataggcgttg cttcttcaaa accagcgcgt tcgactgatt tcaacaattc tggtgataat
     5041 tctagttctt taaatttcaa atgaatcctc ctatatggtt gttaactgag gatgtagctt
     5101 ttttgctacc ccgtcgattt gttcggtcaa agtgtattgt ttctttttga cagtggccac
     5161 gcacgttttt atccattctt agcccctgtg gcaccaccga aaaatcactt tgcgaagttt
     5221 aggcattaga aaagccaaac gggtcgcgtt cagctttatc attattatga catctggctt
     5281 caacaagttg tttctatggc aaaaccagtc caatttctct tcatctttcg gcaataggtt
     5341 attctaacat agtttttcgt tttctgcaag tttacagatt tttatttcat ttgctgaagt
     5401 tgttctacca cttcaaataa gcccatgctg ttgctgcctt ttagcataat actatctgtt
     5461 gctgcaagat tttgtttgat agcatcaatc aatttattct tttctttttc aaaatagaac
     5521 acaggtagtt gggggtattt ttggttcaat tcttctttta gtgcatgcat ttgttcccca
     5581 taaaggaaaa tcatggcgaa tttctcagga tcgatatgtt cactcatttg cgcatgcata
     5641 tttaaagact ctttccctaa ttccagcata tctgctaaca cagcgattct gcgtcctttt
     5701 gttggcagat ttttaaaagt atctaatacc agtcccatgg ctgttggatt tgcattgtag
     5761 acatcgctca aaatagctgc accatttcct gccgttagcc attcggttct gttttgagtg
     5821 atagaaacat gggcgagccc ttggcgtatc tcaggtatgg acagtccaaa gaagcgcccg
     5881 atcgtatacg caatcaaagc attcgttaca ttataacttc caggcagcgg gatcgtaaat
     5941 gtttctcctt ctactacaaa atcggtttgt gcttttcctt ttgctgtcac ttccgcatga
     6001 acatcgccat ttccaatacc gaatgttgtg actgtttgta ctaatgttgc cgttaaaggt
     6061 gtcagcaacg gttcatctgc gggcacaacg agtaatccgt ctgatacaag tccgtcagtg
     6121 atttccattt tggctttagc gattccttct cgcgacccaa gattttctat gtgtgcttca
     6181 ccgatcattg tgattgctgc agcttttggt tgagccataa gtgaaagttc cgtgatttca
     6241 ttggcgtgat ccatgcccat ttccaaaacg atcttttctg tatcttgggg catgtgcaaa
     6301 atcgtgtaag gaagaccaat attattgttg taatttcctt gcgtcttata tgttttgtat
     6361 ttttcagaca agacagacgc ggttagatct ttcgtggtgg ttttaccatt gcttcctgta
     6421 attgcaacga cttctgcgcc tactttttcc aagtaatagg ttgccaattg ctgcatggct
     6481 ttcaaaggat ctgctacagg aataactgga aaattttttg gagcttgttc aacaggcaag
     6541 ctccaaaatg aagcagatgc tccgtttgtg atcgcttgct ggataaattc atgtccatct
     6601 cgcgtaccag caagaggaac gaataaactt ccttcggtta tcttccggct gtcaaattcg
     6661 acattggtaa tctttgtcag tgcagagcat tcgctgccca cggcttgtgc aatttcttgt
     6721 attgttagat tcattgagat ctctcctaaa aaaggttgtg aaaaaagcgt ttggcctaaa
     6781 cggcgggttc gcatgactct cacttccaag taaatttctc tattcgtatt tagaagctag
     6841 aatgaacgcg gttacgcaat cactccagcc tcttctttat ttctctttat tcattttttt
     6901 caaaaaaaga ttgacgctga tggtatcgat tcattcctaa ctggatcagt tcttcaatca
     6961 aatcaccgta tttcaagccc atattttccc ataagagtgg gtacatactg aactccgtaa
     7021 atcctggcat agagtttaat tcattcagga ataattcatt tttatttgtc aaaaagaaat
     7081 cgcaccggct caatccgctt ccacctaaca tcgtgtaagc taacttcgcg tactcttgcg
     7141 ctttttgata aacttcttct ggcacttcgg ctggaatctg catttcgatt ttattattga
     7201 tatattttgc ttcataatca tagaatgcta cgtcttttac gacttcacca ggcaaagtcg
     7261 tccgaacgtc ttcatttcct aatacagcaa cttcgatttc gcgcgcttca attccttgtt
     7321 caacgattgc tcgagaatca tactgatagg ctgttgctaa agcattttgc agctcttctc
     7381 ggttttctgc ttttgtaatg ccgacactag aacccatatt cgccggtttg acaaacatcg
     7441 gataaagcaa agaaccttca cattgatcaa atactttttt aggattttct ttccattgat
     7501 tcttaagtac tggtacataa ggaacttgcg gcacaccagc agcttgtaaa atatacttgg
     7561 tcatgatttt atccatggca catgcactgg tcaatacgcc tgcgccgaca taaggcatat
     7621 tcaatgtctc taagaagcct tggatcgttc catcttcccc gtttggccca tgtaaaactg
     7681 gaaaaacgat ggctccttct tctttgattt cgcccggatt gatcactttt cctgtaaagc
     7741 cttcctctgt ctgtccactt gggtcccatg aaagatgcaa gacatctttg ctggcaggtt
     7801 tttctgttag taatggacct ttgacccatt gtccttcttt tgtaataaat acgagttgaa
     7861 cttggtaata attataataa atggcattta aaactgaaaa tgcggaaaga atggacactt
     7921 catgctctgc gctgcgtccg ccatatagta aagtaatctt caaaagattg tcctcctgta
     7981 ttctaaatcg ttcagtgatt tactctcata ttttagcaca aaacaaaaat aaacgaataa
     8041 cttatttata aaaaaacgag ccagcttcta aaagaacgag cgtcatttat tttttgtcag
     8101 ctagattatt tgtcccgttt tttataaaaa gttatgataa agaaaaggag ggatgatgat
     8161 ggcgcaaaca aatccacgca gccgcttggc agaaatcatt cgagtgttca tcaattacaa
     8221 tgtagtgcca aactttgtac agcaaaaaaa tcccgaacag gtaaaaaaag cctttgaaga
     8281 actaggcccc accttcatca agataggaca gatgctttct gttcgagaag atttgctttc
     8341 ttctgccttc actcaaacat tcaaaacatt acaagatagt gtacctagcg acacattttc
     8401 aacggtcaaa aagacgatcg aaacagaact ttctctttca ttatcagata tattcgatga
     8461 tttttcaaaa tcgccttttg cctctgcttc tatggggcag gctcatcgtg ccaagctgaa
     8521 gaacggtgac gcggtagtcg ttaagatcca acaccctaac atcgccgaag agatccgact
     8581 tgatcttcaa ctatttgaac gagcgatccc tttgatcaaa tacattcctg aaactagcgt
     8641 agttgatcta aaaggggtac tccaagaagt aaaacgttcc ttgatcaatg aaatggactt
     8701 cttaaaagaa agtcaaaatg gcgaacaatt ctatcaaaag aacaatggct ggaaagagat
     8761 ccgcagcccc aagatttatg acgctttttg ttcgaaaaaa gtgatcgtga tggaagaaat
     8821 gtctggtaaa aacttgaatc atttaatgaa cgccgaaaac aaaactgaaa cttttatcac
     8881 tggaattcaa aacaagcagc tgaaacaaga agttgcaaag cttttagttg agaactttat
     8941 gaaacaggtg tttgatgatg gttttttcca tgctgatcct catccaggga atctgctatt
     9001 ccatgtacta acaaaagaag aacagacaca agctagccga aaaactgaaa cagtccatga
     9061 aaaagagttc ggttcctttg cttttcgagc ctccacttct gcagaagacc ctgttgcgcc
     9121 atataccatc aactacatcg attttggtat gatggggcat ctttctgcag gacttaggca
     9181 aaaactcaca caagccgttt tagcgctgta tacgaaagat gcttatcgaa tcgaaaaagc
     9241 ggtccttcga ctttgtcagc aagaaggctc gtttgatgaa agtcggttcc accaagaact
     9301 gacaagtttt cttgaacagt attatgattc tcctattgat gaaatcaact tgcaagaagt
     9361 attcacacat gtcgtgacga tttgtcatca aaataatctg cagttcgatc gtgatatcac
     9421 gcttttgctg aaagcatttg ggacattaga aggggtgatc cgcgtcctcg atcctgaagt
     9481 ttccctcatg gaagtcgctt cgccttttgc tcaacattat tttctcactc atctggatgt
     9541 agaagataca ttgaaacaaa gcggattaga cttattggaa gggatgaagg ctgcgcctaa
     9601 gatccctcaa cagctccacc atctattaga aatgtggact tcgggacaag gaaaagtcaa
     9661 tttggaattg aaaaagcaag acaaattatt gagcaggatc gaaagtatga tcaatcggct
     9721 tgttttcggt atgattttag ctgcattgat cgtagggtcc tccctgcttg tccaagcagc
     9781 acctgtagaa aacgcagagg tcgtttcctt attagggata tttacgtatg ctattgccgc
     9841 attcgtcatt atttttttgg caatcgatgc attgatccaa atgtataaga agaggaaaaa
     9901 gtagaaaaag gttgtgacag aaagaatcag cttcaagaaa taagaagggg ttttacataa
     9961 attgtttctc aaatttttgt gaaaattcgg cttatttttc gaagaggcta cttctgaaac
    10021 accgagaata aaaaatgtga aaaagctgtt ctgcacccag taataagaac aatcaaaaag
    10081 ggaacatgga acacatttat cctgtttccc tgttcccttt ttgatgtatt cgagcataaa
    10141 taaaatacct tcgttaccta ctatcttctg tacttggtaa accttagtca attgcctttt
    10201 ttaacgcttg acgaaaagac atatgatctt tctccgtaaa gggttttgga ccctttgtcc
    10261 ttatgccagc ttttcttaac tttccactat gataacgttg ttctagaagc gtgccgatat
    10321 tcgcttcgtc aaattctttt ccagagtgat ggaacactcg cacacccttc ggcaatacaa
    10381 gtgaagctaa accataatct tgcgtgatca aaaagtcatt cttcttaacc ataccgacga
    10441 ttttataatc cgcactatca gctccgcggt ctacgtagac gatttggaca tgattagcgt
    10501 attctttggt cgtataatgg tccacactgg tcacgataac cacgtcaaga ccataggatt
    10561 ctcctaattt tatgacatct tcttttactg gagaaccatc tccatcaatg aatagcctca
    10621 ttctttttcg ccttcttcca taaatgataa acagttacta atccgctcag taaaagacca
    10681 acattgatcc attttgccaa gataagcagt tgtacacttt ctatcggcac tgattcatgg
    10741 ttcagcgtga tcaataatag atggattggc tctcccattt cttttgctaa aacaaacaaa
    10801 agcgtcatca ataaaaactg gtggatcaca ctccctttat tttgagtagt atacttgaca
    10861 acgatccggc gatccattgg aatagctgta tcagtttgat aatattcctt atttagccag
    10921 ctgcctgtta ctacgtgcca gaaactaagc agaaagacac ctatcgcaaa accatttgga
    10981 atcaagagga ccaataaaga agaactcagt ccaagcaatt gcatagccaa tggagaccat
    11041 gttaaacgtt tcaaaatcca tggtccgact atttttgctg caatcaagcc tgctgcataa
    11101 ggcaaataca taaaaatcga aagtttttct aatccgtaca gtatgccaac ataaaaactg
    11161 ctaaataaaa aaaggaaatt ccctagcatt ccatttagaa aagtaaacag atttagccag
    11221 cttggcaact tccattcctt cttctggtgt cctaaaacaa acacgaacaa gatgaacagc
    11281 agcaagaaac cataaatagc aagatcaaaa agcctttcat cgaataacaa tcgggcattt
    11341 ctcaaaagaa acaataagcc aaagaatatc aaaaataaaa caagctctcg cgtagctacc
    11401 agatttcttt tgatgtcttt gaaatccagt tcgtaacgcg gataatgact aaccgtatgg
    11461 taggccatga cgtaaaaaag tgtataaata gtaaagcttg ctgcaatctg agtcggtgca
    11521 ggcaactcaa tcgcttgata caggagaaat agccagatag ctgcaaataa gtactttttc
    11581 ccatccatat tgataaaacc ttgcgtcttt tcgtgaaaat tgaccgtggt attagcaggt
    11641 acaagccatg ctgcactcag ccctagcaaa acacttgaaa aataaaataa aggaaaataa
    11701 aatacgccta atattccaag caatgaacca gcaccaccaa tcagcagagc gatcatcagc
    11761 aacgtgtaac tatctagacc aaatcgtaga ctgttgacta aaaatagtcc agtcatccga
    11821 aaggcataaa acagcgtaaa tggcaaaatc gtcagccaat tatgcgagtc tgttagtccc
    11881 aaaaataaaa gataaggaat aaaaataacc gtgttactta agataaaggg cgttgtttgt
    11941 gtaaaattac gcccaaatcg tttgagtttt tcgaacatga caatggctcc ttaaacagtc
    12001 ttataactaa tctgctcttc gattttagca ttaaagggga aataaaccaa ttttacagaa
    12061 tatattcttg taaaaaaaaa tacagaaaga agcataagct tctttctgca tttttacggt
    12121 acttgattac tcaagcagac aagcccttct ccacaaaagt cttgcccgac acccctgcat
    12181 ccggcaggtg ataaagatag aaatcaatta tcctgtgcct ccaaaatcaa ttaggagctc
    12241 tgtaaaccag agtccttctc gacccgtcgc ataaatttac tttaacacag ttcctatgtt
    12301 tatgcgagag cttttttcat tttataatcg accatttgaa atccttgacc gatatctgaa
    12361 ataagttctt ctgtaatctc aaatccttca tgcttataaa cctcgattgc ttgatgattc
    12421 ccctgattca ctcgcaaaaa gatctctctt tcctttgttt gcgcagcaag ttcttcatac
    12481 cagcgaaaaa ctgaagaagt caatccttga ccacgtactt tatttagcaa atataattta
    12541 cttaaataaa tttttccatt tatctcttca taagcagtgt aaccgacagt cttttcttcg
    12601 ctttttaata aaaagtagga cacaccttct gtcagttctt tttgaatctg tttttttgac
    12661 tgataagttt tcagcatgta atccacttgt ttttgaccaa tgatcggcac aaaagcttcc
    12721 ggccatactt cttgtatgac tgccatcagc tcatccagtt cattttctgt tacttttatt
    12781 ttttccatac acccacgtcc ttttttacat tataaatgac cttgcgcttg atggaataaa
    12841 aagagcatga tcatgctata cacacccacc gaccatggtt tcaagaaaat cacaatctgc
    12901 ataaattccc taaaagataa tttcgtcaaa ccagctacta gacagatcaa gtcgtctggc
    12961 gcaaagggaa gcagcaaggt aacaactagt acttttttaa tatctttcgt ttttcttgtc
    13021 agcaattcat caaatttctg atactttttt ggcgataaaa taagctggac aaaggctacg
    13081 ccatagtaac ggacaagcaa aaatccgatt gcttccccaa tcactaggcc aatacaacta
    13141 tataaaagcc caaatccatt gccaaacatc agcatgcccg ctactgagga gatccctccc
    13201 ggcaaaatag gaatgatcac ttggatcact tgcaacagaa caaatatcat cactgcatac
    13261 ttgccaaatt gctggataaa cgcttgcaaa gacgtgctcg atcgaaaaat cccatgatga
    13321 taagcataaa gaatcaacac aaagaaaaaa aacagaccaa taatgggcat gatctgaaga
    13381 catttttgta aaactgatcc ttttttagac atctatattc cccacatctt ttttcattta
    13441 ttaaaactgc ctcctt
//
LOCUS       DACGLE010000073        13434 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0534
            SAMEA3304449-rid9560453.denovo.077, whole genome shotgun sequence.
ACCESSION   DACGLE010000073 DACGLE010000000
VERSION     DACGLE010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304449
            Sequence Read Archive: ERR985064
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13434)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13434)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702225.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702225.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 20:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,045
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 27 of 67
            Pseudo Genes (incomplete)         :: 25 of 67
            Pseudo Genes (internal stop)      :: 19 of 67
            Pseudo Genes (multiple problems)  :: 4 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13434
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304449-rid9560453.denovo.077"
                     /strain="VREN0534"
                     /db_xref="taxon:1352"
     gene            41..1858
                     /gene="acm"
                     /locus_tag="IXT20_002238"
                     /pseudo
     CDS             41..1858
                     /gene="acm"
                     /locus_tag="IXT20_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289238.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Acm"
     gene            complement(2130..2615)
                     /locus_tag="IXT20_002239"
     CDS             complement(2130..2615)
                     /locus_tag="IXT20_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HAQ4860669.1"
                     /translation="MSRATEQEAYELLKKLGISFQKVDHPAITSVKNVPFSLPGPQVK
                     NLLLKSRKGKQIYLVILPDEKHADLKQLAEQLKEKRLSFVSEEQLPDLLGVPAGTVTP
                     LALMHDKEKKIQVVIDAAIDRQDTVGFHPNINTTTLIMDFSDFEKILVYLEHLPVYEN
                     L"
     gene            3095..3736
                     /locus_tag="IXT20_002240"
     CDS             3095..3736
                     /locus_tag="IXT20_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAQ4860670.1"
                     /translation="MEYLIEVMPQLLVGALTTLKLFLFTLIGSLPLGIVLALGLSLHF
                     KPLRYLLQTYVWVMRGTPLLLQLIFIFYGLPTIGIVFDRFEAALIAFILNYAAYFAEI
                     FRGGIQSIPQGQYEAAQVLQLSPFQTVTKIVIPQVTKIVLPSVGNEVINLVKDSSLIY
                     ILGLGDVMRAGKIAMERDATLLPLLGVAIIYLTLTGILTIVMKQVEGKLNYYR"
     gene            3755..4390
                     /locus_tag="IXT20_002241"
     CDS             3755..4390
                     /locus_tag="IXT20_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HAQ4860671.1"
                     /translation="MIELKKVNKAFGEKKIIKDLNLIIPDGKTLAIVGPSGGGKTTLL
                     RILAGLESSDSGEFYLDGVPFNPFELKMKEQVVGVVFQDFQLFPHLNIFENITLAPKM
                     VLKQTKEEYTKKAEELAASLGISDLLNIYPYQLSGGQKQRVAIARALAMNPRVLAYDE
                     PTSALDPELRQQVERLILSLKEDGVTQIIVTHDLVFAENVGDQLLKVSPIH"
     gene            4408..5223
                     /locus_tag="IXT20_002242"
     CDS             4408..5223
                     /locus_tag="IXT20_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HAQ4860672.1"
                     /translation="MKKTSFLFALIAGLLLFAGCSNKKTSADNWATINEEKKIVIGLD
                     DTFVPMGFRDKDGKLTGFDIDLAKAVFEQYGITVDFQPIDWSMKEFELNNGTIDLIWN
                     GYSKTPAREKKVQFTKPYMENDQVLITPKESGITNFGQMNNKRLGAQNGSSGYDVFTK
                     QPEVLKEIVKNQDAVLYDSFNEALIDLKSRRVDGLLMDKVYADYYLKQRNELEDFNIT
                     KGSYESEDFAVGARKSDSTLVKKINEALDQLEKTGEFQKISDKWFGEDVTPRN"
     gene            5542..6978
                     /locus_tag="IXT20_002243"
     CDS             5542..6978
                     /locus_tag="IXT20_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAQ4860673.1"
                     /translation="MEHKELTSFPKDFLWGSASAAYQVEGAWQEDGKGESVWDRFVRI
                     PGKTFKGTNGDLAVDHYHRYKEDIALMKEQGLKAYRFSVAWTRIFPNGRGEVNQAGLA
                     FYERLIDELIENDIEPMLTLYHWDLPQALQDEYNGWESRQIIEDFTHYAETLFEAFRG
                     KVHYWISLNEQNIFTSLGYLLAAHPPGVTDPKRMYEVNHIANLANASVINKFHELEMP
                     GKIGPSFAYTPNYPIDSNPENVLAAENAEDLMANYWMDVYMWGKYPIAAMRFLEEKGW
                     APTIEAGDAELLESAKPDFLGINYYQTATNAFNPLNGVGAGKMNTTGKKGSSEETGVP
                     GMYKKVENPFVERTNWDWEIDPEGLRIGLRRITSRYRMPVLITENGLGEYDKLTEDKQ
                     IHDDYRINYLQSHVKAIKEAISDGAEVLGYCTWSYTDLLSWLNGYQKRYGFVYVDQDE
                     TQEGSLERYKKDSYYWYQKLIEENGENL"
     gene            7167..9614
                     /locus_tag="IXT20_002244"
     CDS             7167..9614
                     /locus_tag="IXT20_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HAQ4860674.1"
                     /translation="MAQKKRPTKKRKKTKKTKKQKQQQEQMLTIGVGFALILFAVFGF
                     LKLGFLGILIANGFRIIAGNTYQILCLLLAVLGFWIVIKNTEFSIGKNRRWFGGILFY
                     FGILLLLHAHLFGKLHTGEPNIMGTTWDLLASDIKQSQVDNNVGGGMIGAILYHFTYF
                     LIAQPGSYLAAVLLLAGGAFLFSNLEGYQLLNGIQSIGERVQELLEGDPAKQARKQAA
                     KEERMKQRAEAKEARRLAAKEAAEKEAAEYEKKKAVSQQRENTADEWQEPENTEPEQL
                     SFVPIDSFQENIHPANLEKPVPDTPKQTNTAEGFSDELPEDDGTSLEFEIEAEQENQD
                     YELPTVDLLDSIPTVDQSDEYKKIEKNIGVLEQTFQSFGVDAKVVKASLGPSVTKFEV
                     QPAVGVKVSKIVNLTDDIALALAAKDVRMEAPIPGKSLIGIEVPNGKISMVSFREIIE
                     AQPNHPDKLLEVPLGRDVSGRVQTADLSKMPHLLVAGSTGSGKSVAINGIITSILMRA
                     KPHEVKLMMIDPKMVELNMYNGIPHLLTPVVTNPRKAAQALQKVVQEMEERYEKFAAT
                     GVRNISGYNEFVQQKNLENGTKHPTLPFIVVIVDELADLMMVASNEVEDAIIRLAQMA
                     RAAGIHMILATQRPSVDVITGIIKANVPSRMAFAVSSGTDSRTIIDSNGAEKLLGRGD
                     MLFLPMGENKPIRVQGAFISDHEVERVVQFVTDQQEAHYEEKMMPTDEVETTGAPEQP
                     QDELFEEAKALVVEMQTASISLLQRRFRIGYNRAARLVDELEAHGVVGPSEGSKPRKV
                     FIEQEETSETLPDDNDM"
     gene            9844..10911
                     /gene="rlmN"
                     /locus_tag="IXT20_002245"
     CDS             9844..10911
                     /gene="rlmN"
                     /locus_tag="IXT20_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HAQ4860675.1"
                     /translation="MEKQSIYGLTNEELINWFIENGEKKFRAAQVWEWLYQKRVSNFT
                     EMTNLSKQLIEKLSEHFIINPLKQMVVQEASDGTVKYLFELPDKNMIETVLMRQEYGL
                     SVCVTTQVGCNIGCTFCASGLLKKNRDLTAGEIVAQIMMVQHYFDERNLGERVSHVVV
                     MGIGEPFDNYDNVMDFLHIINDAKGLAIGARHITVSTSGLAHKIKEFANNGLQVNLAI
                     SLHAPNNEVRTSIMRINRSFPIEKLMEAVDEYLEKTNRRITFEYIMLNQVNDRPEHAQ
                     QLADLLKDKKKLAYVNLIPYNPVSEHDQYSRSPKADVLRFYDVLKKNGVNCVIRKEHG
                     TDIDAACGQLRSKQMKKTVAK"
     gene            complement(11104..12207)
                     /locus_tag="IXT20_002246"
     CDS             complement(11104..12207)
                     /locus_tag="IXT20_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HAQ4860676.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
     gene            12760..13308
                     /gene="aac(6')"
                     /locus_tag="IXT20_002247"
     CDS             12760..13308
                     /gene="aac(6')"
                     /locus_tag="IXT20_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HAQ4860677.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT         4582 a         2252 c         2852 g         3748 t
ORIGIN      
        1 aagaaaccgt taggtttagc taagtggtga gtagttcact gaaaatatta aaaacctaga
       61 ctcagtagaa ggttgggggc aatttgagat acaggccaga aacgtaaccg ataccggtga
      121 agaaaatacg ggaccattca ccgtgacgag cggtgataaa acagctactg taaatgtgac
      181 aaagccagct tctggttctt cttctagcgt tttttattat aaaacaggag acatgctacc
      241 tgaagatact aagcacattc gatggttttt gaatattaac aataacggca cttatgttga
      301 acaaccagtt aaaatcagtg atgaaatcca aagtggacaa agattagatc caagtacgtt
      361 tgaaattaat caaatacact taggtgaaca gaaggtttat agaggagaag agggaatcca
      421 acaattttta caagatttcc ccagcgctac atttaatttt tcagtaactg ataattacat
      481 tgaaataact ataccaaaaa actttgtcaa tcttaggaaa ataatggtga gttataagac
      541 gatcatagaa aacccagaac aaataaattt tgagaatcat tcggaagctt ggtttaaaga
      601 atttaataaa cctgctgttg atggagaaag tttcaatcat acagttaaaa atatttcagc
      661 ttctggtgga gtcaacggta cagttagagg cgaattgaag attttcaaat atatcaatga
      721 cacagagatt ggtatcccaa atgtgacttt cgaattaaga cgagcagatg aacagcctat
      781 tcaaggacag tctagtatcc tgttgacttc gaatgaacag ggagaagcta gtatcaaagg
      841 cctgcaagta ggagactatg ttgtaaaaga aaaagaagca cccaattgga ttgattttga
      901 tccactaagt tcaaatgaac tcaaattctc cataaacgaa aatgatacag agggagtcag
      961 tttacctatt tataacaaga aaaaggttac gaacatcaca gcaactaaaa tttggaacgg
     1021 tggaaccact ccaagaccga gtatttattt taaattattc agagcaagta caaacaattg
     1081 ggaaccagtt cctgatgcag aaacaaaacg attagacaat ggaataactt cagtgacttg
     1141 ggaagatatc caacagtatg atgatagtgg aaacgaatat acgtttaaag tgcaagaagt
     1201 agatcaaaac ggaaatgatt atgtcccttc aggttatcaa aaaattgaaa acgggctagt
     1261 cgttacaaat gagaataaag aagtcatttc agtctcagga caaaaaacat gggaagacaa
     1321 tgagaaccaa gacggcaaac gaccgacgac gatcacagta aatctattag ccgacggtca
     1381 accgatccaa cacaaagaag taagtgaaaa agatgattgg cgctatcgtt tcacaaatct
     1441 gccaaaatac agagacgggc aagaaatcat ctatactgta acggaagaca acgtgcccga
     1501 atacagtacg acgattgacg ggtataacat caaaaacagt tatacaccgg aaaaaacaaa
     1561 tatatctata ccaggtagcc atataacacc gtggaaaaat tttccagaga agacagagag
     1621 agagaaaatt agaaatctat tctctcaaaa attgcaaata tttgctcata caggagaatc
     1681 cagcgaacaa agaagcaatt atattagtaa gagagctgta cctaaacaga taacaaataa
     1741 atcaaaaagc attcttccta aaacaagtag taaaaaatct agttttgctg taatcattgg
     1801 tttattgata gtaactatct gtgctgggat attcttacaa aaatataaaa aagtttagtt
     1861 agaatataat attttttgtt aaatatgact gaaatcaaaa gaataaaatt ccgatttata
     1921 atcgaaaaaa gaagcatttt tatatatgct tcttttttcg tagaatcaat aaatatggat
     1981 ttggctaact atggaagaat actcgtatta gttttcagcg ttcatactag ttgttacatt
     2041 gtttgattgg ccaaaagaat ttctgatata ttttgtgcta aataaagaaa aagacgcttg
     2101 aattgggaaa taagaaaact actctatcat tacaaatttt cataaactgg cagatgttct
     2161 aagtaaacaa gtatcttttc aaagtcggaa aaatccataa tcaacgtagt ggtattgata
     2221 tttggatgaa agcctacggt atcttgacga tcaatagcag catcgatcac gacttggatt
     2281 tttttctctt tgtcgtgcat caatgctaat ggagtcactg tacctgctgg aacgcctagg
     2341 aggtcaggaa gctgttcttc agaaacaaaa gataagcgct tttcttttaa ttgttcagct
     2401 agttgtttta aatcggcgtg cttttcatca ggcaagatga caagatagat ttgttttcct
     2461 tttcttgatt tcaataatag attttttact tgcggaccag gtagggaaaa agggacattt
     2521 ttgactgacg taatcgcagg atgatcgact ttttgaaaag agatacccag tttttttagt
     2581 agttcataag cttcttgttc ggttgcacga ctcatcgcgc atcaccctcc tttttttatt
     2641 agtgtatcat aaaaaagagt tgacaaatga aaaataatca tgtaaattac acgtaaagtt
     2701 aaaacaaatg acatcgaaaa gaagagtagc cgttaggtgt ttttagagag tctctggttg
     2761 gtggaaagag gcagcagtta accgtgaaac acatcttgga gtcgattttc tgaagcgtat
     2821 agtaggaaaa tccggggagc ccgttacagc tgcggtcgtt gctagatgac cagataaggc
     2881 tggcttttcc gtgaggagtc ggtaaaaaaa ggtggaacca cgtgattaac gtcctttgat
     2941 cttcggatca aaggactttt tgttttcatc tgaacggcaa cgactgtgtg aggttttttc
     3001 tgcaaggaaa aaataaacag aggtggaacc acgtataata cgtcctcttg gcatttgcca
     3061 agaggatttt tttattttaa aggaggaaaa gaagatggag tatttgattg aagttatgcc
     3121 gcaattatta gtaggagcac tcacaacatt gaaattattt ttatttacct tgattggctc
     3181 attaccatta gggattgttt tagcattagg attgtctttg cattttaagc cgctacgcta
     3241 cttgcttcaa acatatgtat gggttatgcg gggaacaccg ctgttgttgc aactgatttt
     3301 tattttttat gggttgccga cgattgggat cgtttttgat cgtttcgaag cagcactcat
     3361 tgcctttatc ttaaactatg cagcttattt tgcagagatc tttcgtggag ggatacaatc
     3421 gattcctcaa ggtcaatatg aagcagcgca agttttgcaa ttaagtccct ttcaaacggt
     3481 gacgaaaatc gtcatcccac aagtgacaaa gattgtttta ccttctgtag ggaatgaagt
     3541 tatcaacttg gtcaaagatt cttccttgat ctatatatta ggtttaggtg acgtaatgcg
     3601 tgctgggaaa atcgcgatgg aacgcgacgc gactttactg cctttgctag gcgtggcaat
     3661 catctatctt acattgactg gtattctaac aatcgtaatg aaacaagttg aaggaaaatt
     3721 aaattattac cgctaactga taggaggaag aaatatgatt gaattgaaga aagtgaacaa
     3781 agcatttggt gaaaaaaaaa taatcaaaga tttgaatcta atcattccag atgggaaaac
     3841 attagctatc gtaggtcctt ccggtggcgg taaaaccacg cttttacgta tcttggctgg
     3901 cttagaaagt tctgacagcg gagaatttta tttagacggt gtaccgttca acccattcga
     3961 gcttaaaatg aaagaacaag ttgtaggagt cgtattccaa gacttccagc tatttccaca
     4021 tctgaatatt tttgaaaaca ttaccttagc accaaaaatg gtattgaaac agacaaaaga
     4081 agaatacacc aaaaaagcag aagaattagc ggcttcatta gggattagtg atttattgaa
     4141 tatctatcct tatcagcttt ctggcggaca aaaacagcgg gtagcgattg ccagagcatt
     4201 ggctatgaat ccacgggtat tggcttatga tgaaccaaca agtgctttag atcctgaatt
     4261 acgccagcaa gtcgagcgtt tgatcctatc attgaaagaa gatggggtaa cccaaattat
     4321 tgtcacccat gatttagtat ttgcagaaaa tgtcggtgat caattactaa aagtttcccc
     4381 aattcattaa gaaaggagaa gatagagatg aaaaaaacca gttttctatt cgctttgatc
     4441 gcaggtcttc ttctttttgc aggctgttcg aacaaaaaaa cttcagcaga taattgggcg
     4501 actatcaacg aagagaaaaa aatcgttata ggtttagacg atacatttgt tcctatggga
     4561 tttagagata aagacgggaa gctcacagga tttgatattg atttagccaa agcagtcttt
     4621 gaacaatatg ggattacggt agactttcag cccattgact ggtcaatgaa ggaatttgaa
     4681 ctgaacaatg gaacgatcga tttgatatgg aacggctatt ccaaaacgcc ggcaagagaa
     4741 aagaaagtgc aatttacaaa accttatatg gaaaacgatc aagtgctgat cacacctaaa
     4801 gaatcaggaa tcacaaactt tggacaaatg aataataaga gattaggtgc gcaaaatggg
     4861 tcatctggtt atgatgtatt tacgaaacaa ccagaagtat taaaagagat agtcaaaaac
     4921 caagatgctg ttttgtatga tagttttaat gaagcattga tcgatctgaa aagcagacgg
     4981 gtcgatggtc tgctgatgga taaagtatac gcagactatt atttgaaaca aagaaacgaa
     5041 ctagaagact tcaacataac aaaaggttct tatgagagtg aggattttgc agttggtgca
     5101 cgcaagtcag atagtacatt agtgaaaaaa atcaatgaag ccctagatca actagagaaa
     5161 acaggtgaat tccaaaaaat atccgataaa tggtttggcg aagatgttac gccgagaaat
     5221 taacaaatag gggactgtga caaaagacgt gtcacagtcc ctttttccga ataaacagtg
     5281 ttctcctgcc aactccttgt gactcggagc tgaatggcag gctcacaacc tcttggtaaa
     5341 cggtgttcaa aagccagttt ttcggtatta agccaaattt atgcaaaaat gtagaaagcc
     5401 attttcacaa aatttttctt actactcgaa actaagcgac tttttcacaa cctcctttac
     5461 atatttttat tttgttgtgg aaggggtttc gtgttattct aaaagaggaa cgattgaagg
     5521 ttataggaaa ggaagagatg aatggaacac aaagaactta cctcatttcc aaaagatttt
     5581 ctgtggggct ctgcttctgc agcgtatcaa gtagaaggag cttggcaaga agatgggaaa
     5641 ggagaatcag tctgggatcg atttgtacgg atacctggaa agacatttaa aggaacgaac
     5701 ggtgatttgg cagtagacca ctatcatcgt tataaagaag atattgcttt aatgaaagaa
     5761 caaggtctga aagcttaccg cttctctgta gcttggacac ggatttttcc taatggacga
     5821 ggagaagtca atcaagctgg gctcgctttt tatgagagat tgatcgacga attgattgaa
     5881 aacgatattg aacctatgtt gactttatat cattgggatc ttccacaagc attgcaagat
     5941 gaatataatg gttgggaatc acgtcagatc attgaagatt tcactcatta tgcagaaaca
     6001 ttatttgagg cattccgcgg gaaagtccat tactggatta gtttgaatga acaaaatatc
     6061 ttcacttcat taggttattt gttagcagca catccgccag gtgtgacaga cccgaaacga
     6121 atgtatgaag ttaaccatat tgctaattta gcaaatgcat ctgtcatcaa taaatttcac
     6181 gagttagaaa tgcctggaaa aatcggacca agctttgcgt atacgccaaa ttatccaatt
     6241 gacagtaatc ctgaaaatgt tctggctgct gaaaatgcag aagacctgat ggccaattat
     6301 tggatggatg tctatatgtg gggaaaatat ccgattgctg ccatgaggtt cttagaagaa
     6361 aaaggatggg caccaacgat tgaagctggc gatgcagaat tgctagaatc tgctaaacca
     6421 gattttcttg ggatcaatta ctatcaaacg gcgacgaatg cattcaatcc attaaacgga
     6481 gttggtgctg gaaaaatgaa tacgacagga aaaaaaggtt catcagaaga aactggagtt
     6541 cctggaatgt ataaaaaagt tgagaatcca tttgtcgaac gaacaaattg ggactgggag
     6601 attgacccag aaggattaag gatcggactg cgtcgtatca caagccgtta ccgcatgccg
     6661 gtactgatta cggaaaatgg cttaggagag tatgacaagt tgacagaaga taaacaaatc
     6721 catgacgact atcgaatcaa ttacttacaa agccacgtca aagccatcaa agaagcaatt
     6781 tcggatggag cagaagtcct aggctattgc acctggtctt atacagattt gcttagctgg
     6841 ctgaacggtt atcaaaagcg atatggattt gtatatgttg atcaagacga aacacaagaa
     6901 ggatctttgg aacgatataa gaaagacagt tattactggt accaaaaact gattgaagaa
     6961 aatggagaga acctttagtt cacatactta aaacaagaag gtacggctgg atatggccgt
     7021 gccttttctt gataaaatca tttagaaatt aagaaaaact gtaaaataag ctgaaaaatg
     7081 aaagaaagcg tcagttcttt caaacaaaac tttagttccc ttactttttt ttgtataatg
     7141 aaacttgatg aaatgaggga taataaatgg cacaaaagaa acggcctact aaaaaaagga
     7201 aaaagacgaa aaaaacgaaa aagcaaaaac agcagcagga acaaatgctg acgattggtg
     7261 ttgggtttgc cttgatttta tttgctgtct tcggcttttt aaaattaggt tttttaggaa
     7321 tattgattgc aaacggcttt cggatcattg caggaaatac ttaccaaatc ctctgtcttt
     7381 tattagccgt attaggattt tggattgtca ttaagaatac agagttttca attggtaaaa
     7441 accgacgctg gtttggcggc atactctttt atttcggtat cttattattg ttacatgccc
     7501 atttatttgg caaactccat actggggaac caaatatcat ggggacgaca tgggatttgt
     7561 tagccagtga tataaaacaa agtcaagtag acaataacgt tggtggtggg atgattggag
     7621 caatccttta tcattttact tacttcttga ttgcacagcc tggaagttat ttagcagcag
     7681 tgctattact ggcaggagga gcattcctgt tcagtaatct ggaaggctat cagctgttaa
     7741 atggtatcca atcaattgga gaacgagtac aggaattatt ggaaggtgat cctgccaagc
     7801 aagctcgaaa gcaagcggca aaggaagaga gaatgaaaca gcgcgcagaa gcgaaagaag
     7861 caaggcgctt agctgcaaaa gaagctgcag aaaaagaagc tgcagaatat gagaagaaaa
     7921 aagcggtgag ccagcaaagg gaaaatactg ctgatgaatg gcaggaacca gaaaatacag
     7981 agccggagca gctaagtttt gttccaatcg acagttttca agaaaatatc catcctgcga
     8041 atctagagaa accagtacca gatacaccaa aacagaccaa tacagcagaa ggtttttcag
     8101 atgagttgcc agaggatgac ggtacctcat tagaatttga gattgaagca gaacaggaaa
     8161 atcaagacta tgaattaccg acagttgatt tgcttgattc tataccaact gtggatcaaa
     8221 gtgatgagta taaaaaaatt gaaaaaaata tcggcgtatt agaacaaact ttccaaagtt
     8281 tcggtgtcga tgccaaggtc gtcaaagcca gcttaggacc atccgtcaca aaattcgaag
     8341 tccagccggc tgtaggggta aaagtaagta aaattgtgaa cttaacagat gatatcgcgc
     8401 ttgctttagc ggcgaaagat gtccggatgg aagcaccgat tccaggtaaa tcactgattg
     8461 gtattgaagt accaaatgga aagatcagta tggtttcctt tagagaaatc atcgaagcac
     8521 aaccaaacca cccagataaa ttactagaag tacctttagg acgtgatgtt tctggacggg
     8581 tacaaacggc tgatctttcg aagatgcctc acttactcgt ggctggttcg actggtagtg
     8641 ggaagtcagt agcaattaat gggattatca caagtatatt aatgcgtgct aagccccatg
     8701 aagtaaaact gatgatgatc gatccgaaaa tggttgaatt gaatatgtac aacggcatac
     8761 ctcatctgtt gacgccggtc gtgacgaacc ctagaaaagc agcgcaagcc ttacaaaaag
     8821 tagtacaaga aatggaagaa cgatatgaaa aattcgcggc aaccggagta agaaatattt
     8881 ctggttacaa tgaatttgtt caacagaaaa acttggaaaa cggaacgaaa catccaacct
     8941 taccattcat cgtagtgatc gtagatgaat tagctgattt gatgatggta gccagcaatg
     9001 aagttgaaga tgcgattatt cgtttagcgc aaatggctcg ggcagcgggg attcatatga
     9061 tcctagctac tcaacgtcca agtgtggatg ttatcacagg aatcatcaaa gcaaatgtac
     9121 cttccagaat ggcatttgcg gtatcaagcg gtacagattc tcggacgatc attgattcta
     9181 atggggcaga aaagctctta gggcgaggag atatgctatt cttgccaatg ggtgagaata
     9241 aaccgatccg tgtgcaagga gcattcattt ctgaccacga agtagaacgg gtcgttcaat
     9301 ttgttacaga tcagcaagaa gctcattatg aagagaaaat gatgccgaca gatgaagttg
     9361 aaacgacagg tgctccagaa cagccacaag atgagctgtt tgaagaagca aaagcacttg
     9421 tcgttgaaat gcaaacggca agtatttccc ttctccaaag aagatttcgg atcggttaca
     9481 accgagcagc acgtttagta gatgaactgg aagctcatgg agttgttggc ccgtcagaag
     9541 gaagcaagcc tcggaaagta ttcattgagc aggaagaaac atcagaaacg ttgccggacg
     9601 ataatgacat gtaaaacttg tgtgatgaag ggttgaaaaa cgaataacca ataaaaagac
     9661 aaggcatgat gataccaaaa gaaaaaatgg tttttttcca tcttttctaa tacggtatga
     9721 tgtcttgtct ttttagtttt tgtgagatta aagttctatt cgatttattt gtgaagtatt
     9781 gcattaagga aatgaatcat gtatagtatg tagcgaaact ttgagaaaaa gagggaaaat
     9841 actttggaaa aacagtccat ttatggcttg acgaatgaag aattaatcaa ttggtttata
     9901 gaaaacggcg agaaaaaatt ccgagctgcc caagtttggg aatggttgta tcaaaaacgt
     9961 gtaagtaatt tcacagaaat gacgaatcta tcgaaacaat tgatcgaaaa attatcggag
    10021 cattttatca tcaatcctct aaaacagatg gtcgtccaag aagcttcgga tggtacagta
    10081 aaatatttgt ttgaactccc tgataaaaac atgattgaga ctgtactgat gagacaagaa
    10141 tatggcttgt cagtttgtgt gacgacacaa gtaggatgta atatcggctg tacgttttgt
    10201 gcaagcgggc tgctcaaaaa gaaccgtgat ctgactgctg gagaaattgt tgcacagatc
    10261 atgatggtcc aacattattt tgatgagcgc aatcttggcg aacgcgtttc acatgtcgtt
    10321 gtaatgggga tcggtgagcc atttgataat tacgacaatg taatggactt tttgcatatc
    10381 atcaatgatg ccaaaggatt ggcaattggt gctcggcaca ttacagtttc tactagtgga
    10441 ttagcacaca aaatcaagga attcgctaat aacggcttgc aagtcaatct ggctatttcc
    10501 ttgcacgcgc cgaataatga ggtgcgaaca tcgatcatgc ggatcaaccg aagtttccca
    10561 attgagaaat tgatggaagc tgtggatgag tatttagaga aaacaaaccg tcgaatcacg
    10621 tttgaatata tcatgttgaa tcaagtaaat gatagaccag agcatgcgca acaattagca
    10681 gatctattaa aagataagaa aaaattagca tatgtcaact tgattccata taatccagtc
    10741 agtgaacatg atcaatattc tcgcagtcca aaagctgatg tattgagatt ttatgatgta
    10801 ttgaagaaaa acggcgtaaa ctgtgtgata cgtaaagaac atggtacaga tattgatgca
    10861 gcttgcggac aattgcgaag caagcaaatg aaaaaaacag ttgccaaata atcgtatcct
    10921 actcgttttt tcaattacca taaaaggagc agttttctta attaaggaac tgttcaactt
    10981 aaataagaga aaagattcta tagcgaaagc tataaagctt tttttgacaa gaaaaaccct
    11041 catcaagcaa agaaatgtcc tcaatttgat gacagcctgc ttgatggggg ttttagcaat
    11101 tatttaactg attttcttat atccttgctg gctttcttgt gtagcatgat aagtttctac
    11161 gtcaagaatg gcctgtttaa atttttctcg ggtgatttca taaggcacat gtttaatatc
    11221 atttgttgcc atggctttat ctaagattgc ttctaaatcg gtcgtgacat tgagattcat
    11281 gtcggttaac gatactggaa gttggttctt tttcataaac acatagattt tatcaaacaa
    11341 atcgaactgc tgatccatca tcaataaggt cagtacacca taactgacag atgctccgtg
    11401 aaggtatccc tccatttttt ctagaacagt cgtaccgtaa tagaatgagt gtgccatagc
    11461 agaattatag ttattgttaa ctaaaacaga aaccaatccg gtagtaacaa tgatatccga
    11521 aacgacttgt tcaattgcat atgagggacg atcgtcggca ctatctttca atgcttgcgt
    11581 accataagtc aagagacgat caatgcacat ttttccgatt tggattccta atctatccgt
    11641 gtagcctaga tcatcgccac gagcagaaaa agtagattcg acttctttgg ataaagcatc
    11701 gccaatacct gcccataagt atttatcagg tgcttcggca ataattgctg tatcaatgaa
    11761 ggtgtgtaca ggtggttttg aaggaaagaa gagtccgtcc attacgcctt cttcgttata
    11821 gatgacacat accgcagtga cgggcgaaca gttcgaacca atagtgggaa aggtgaagac
    11881 tggtttagct agtttttcac caatgacttt tacagtatcg caagcacggc caccaccgac
    11941 tgcaaacagg acgtctgctt tttggacact ttcatgctgg atcaactgtt caatatttcc
    12001 ataagttgaa ttacccccgt accagagact ggcgacaacc tctatttcct gtgtcaaagc
    12061 tacttttatt ttgttttctg cctttgaaag tgcagtcttc cctccaatca gcactacttt
    12121 tgtcccatat tctttgacga ttccatttgc tttttcatac gcatctgttc caatggtata
    12181 atttggtaag aaaacacttt gactcatatt catttccttc tttcttcgtt gaaacgggtt
    12241 aaaattttaa agagtgatcg atctgactgc ggctgtagat ggagtagagg tgaacctaag
    12301 acaggataag tgatgaataa ggtggcatga acaccttgaa atcatcaata agtatcctaa
    12361 aaacgaacaa gtacaaaaac attgaaaacg acaataatga atttttgttc ctcttagtat
    12421 ttctaccgat tccctttgct tttttgtaat tatctaaaaa ttctaaaaat taagattatt
    12481 atatgaaaac aggttttatt tgtcaattca taattggttt tttttaaagc aaaagataat
    12541 ctatatcatg atgaacaaac agagtttttt cctccaattg atggctttcc tgatctttcc
    12601 tattttgaat aaagtgtttc tgtataattt ttttatttta taagtaagat aaataatgaa
    12661 agaatagaga tactgattgg tagctaaaga tttttgagcg agaacatttc gttttttcaa
    12721 aaggactgtt agaataagca agaacaaggg ggaagacgta tgataatcag tgaatttgac
    12781 cgtaataatc cagtattgaa agatcagctt tctgatttac tgagactgac ttggccggaa
    12841 gaatatggag acagctcggc agaagaagta gaagaaatga tgaatccaga acgaatcgcg
    12901 gtagcagcgg tagaccaaga tgagttagta ggatttattg gtgcaatccc tcaatacggt
    12961 atcacaggtt gggaattgca tccattagtt gtagaaagct cccgacgaaa gaaccaaata
    13021 ggtactcgat tagtcaatta cttagaaaaa gaagtagctt ccagaggagg aatcacgatt
    13081 tatttaggta cggatgattt agaccatgga acaacgttaa gtcaaacgga cctgtatgag
    13141 catacatttg ataaagtggc ttccatccag aaccttcgtg aacatccgta tgaattctat
    13201 gaaaaattag gttataaaat cgtaggtgtc ttaccaaatg caaatggctg ggacaaaccg
    13261 gatatttgga tggcaaaaac gattattcct cgaccaaatt ctcaataata aaaagaggtt
    13321 gtgacagaag taatcagctt caagaaataa gaaggaattt acgaaaatta cttcttgaat
    13381 ttttgtgaaa ttccaactta tttccgaaga agctactttt ggaacaccgt gtac
//
LOCUS       DACGLE010000074        13427 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0534
            SAMEA3304449-rid9560453.denovo.078, whole genome shotgun sequence.
ACCESSION   DACGLE010000074 DACGLE010000000
VERSION     DACGLE010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304449
            Sequence Read Archive: ERR985064
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13427)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13427)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702225.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702225.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 20:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,045
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 27 of 67
            Pseudo Genes (incomplete)         :: 25 of 67
            Pseudo Genes (internal stop)      :: 19 of 67
            Pseudo Genes (multiple problems)  :: 4 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13427
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304449-rid9560453.denovo.078"
                     /strain="VREN0534"
                     /db_xref="taxon:1352"
     gene            complement(<1..912)
                     /locus_tag="IXT20_002248"
     CDS             complement(<1..912)
                     /locus_tag="IXT20_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase family protein"
                     /protein_id="HAQ4860678.1"
                     /translation="MIISTITEAIGSTPLYRFDNQHYSMPKGSAIYAKLEYLNPGGSI
                     KDRLGSYLIKEGFAQGKINEKTTIIEPTAGNTGIGIALAALTYKLRTVFVVPEKFSLE
                     KQQLMKALGAKIIHSPSEQGIIGAISKSKKLAEEISNSYLPLQFENKDNPAAYYHTLG
                     PEIFHEIKENIHSFVAGIGSGGTFAGTSTFLKEKYPDIRIIGVEPEGSVLNGGDPAPH
                     EIEGIGVEFIPPFLSPLSINQIETISDVEGFNYTRQLAREQGLLVGSSSGAAFAAALR
                     EIRRLPPGHRVVTIFPDAADRYLSKNIY"
     gene            1340..1903
                     /gene="efp"
                     /locus_tag="IXT20_002249"
     CDS             1340..1903
                     /gene="efp"
                     /locus_tag="IXT20_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HAQ4860679.1"
                     /translation="MISASDLRAGMTFVQDGKLIKVLDASHHKPGKGNTVMRMKLKDV
                     RSGATYDTTFRPEEKFEKAHIDTKPVQYLYTMDDTAFFMDLETYEQYEIPVETVEEEM
                     KYILENMEVKIQFFGSEVIGVQLPTTVVLRVVETQPSIKGATVTGSGKPATMETGLVV
                     NVPDFIETDELLEINTAEGSYVKRASK"
     gene            2389..3648
                     /gene="lysA"
                     /locus_tag="IXT20_002250"
     CDS             2389..3648
                     /gene="lysA"
                     /locus_tag="IXT20_002250"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HAQ4860680.1"
                     /translation="MTELIIGGVTASTLAEKYKTPLYVYDEEKMRQTMLSFKRGFVSK
                     RFDTKVLYASKAFQTIEMLHLVREYGFGLDVVSGGEIYTALQADFPRDTIYFHGNNKT
                     PEELIYALENDLLHIVADNLMEVELLAELSQRYLKKICIMLRLNVGVEAHTHEYIVTA
                     HIDSKFGMSYESKECQQALQLIKESSYLELEGFHAHIGSQIFDVTAWLAEIDKLTNYL
                     TDFDQPLSLNLGGGFGIRYTQNDQPIPVEEALKKLIEYTEEALEKRKLTIRQLLIEPG
                     RSIVGEAGTTLYTIGFIKKTPHKKYYFVDGGMTDNIRPALYQAEYDCDLAAKLSEEKT
                     EKVTIAGKMCESGDVVIKEAYLPKAESGDLLAVYSTGAYGYSMSSNYNRATRPAVVFV
                     NNGQSRLIVRRQAFSDLLRGEVSYDPS"
     gene            3635..4435
                     /gene="dapF"
                     /locus_tag="IXT20_002251"
     CDS             3635..4435
                     /gene="dapF"
                     /locus_tag="IXT20_002251"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HAQ4860681.1"
                     /translation="MILHKYNGCGNDFILLDYAEDTDYSQLAASLCTKEKYDIDGLIA
                     VKIDPLEMIYYNKDGSRAPMCGNGIRCFARYVNDQNMMNEQEFNVETLAGTRKITVLD
                     PSPFYCSVDMGVPDDSPEKIKANQTYPIKNQEIEINGEKVTITSLFLGTIHTVVFVEN
                     AREEVWRKRGELICHHPLFKEKTNVNFVEVVNEQELIVRTYERGVGWTLACGTGCCAS
                     YVVAKETGKISADQVVVHLEQGDLMISGEDAIQMAGPAVHEWTRILEE"
     gene            4438..5319
                     /locus_tag="IXT20_002252"
     CDS             4438..5319
                     /locus_tag="IXT20_002252"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HAQ4860682.1"
                     /translation="MIKGSIVALITPMNEEGSVDYAGLEKLIQFHLDEQTDGLLVLGT
                     TGESSTLTQSEEEQILQLTVKKVAGRVPVIAGAGTNNTKETIEKAKHFASLGADALLV
                     ITPYYNKTSDAGLAAHFTAIAEASPIPLILYNVPSRTGMSIPIHVLVNLAEHPNIIGL
                     KEASGDMAYVMDAARLIGEEFFLYSGNDDLILPVMSVGGSGVISVWANIQPKIVHELV
                     KDTQDGRWQQAKEKQLNALELIHALFSETNPIPVKAAMSLLDLPSGPLRLPLVSLSKE
                     KKKQLAQLLLKERTVEK"
     gene            5316..6032
                     /locus_tag="IXT20_002253"
     CDS             5316..6032
                     /locus_tag="IXT20_002253"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HAQ4860683.1"
                     /translation="MKIGLIGSGQMGNRVEEVAQERGDTVLLLRTAPKEATTLAFTEL
                     PELLIDFSHPDNLEKILSYSLSYQLPLVIGTTGYTQSQFQEIKEHAKHVPVMYSANFS
                     FGIMVMNQLIKQAAAMLQGWQIELIEKHHSRKKDAPSGTANMLLQTIQEVQKLQPVYN
                     WQGKKREENQIGVHSIRAGSLPGEHDVLFAATDEIFTIKHESFSNRIFAAGAVEAADW
                     LKDQSPGYYKLEDLLMDKGV"
     gene            6038..6733
                     /gene="dapD"
                     /locus_tag="IXT20_002254"
     CDS             6038..6733
                     /gene="dapD"
                     /locus_tag="IXT20_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HAQ4860684.1"
                     /translation="MEAQEIIRYIQTASKKTPVKVYLNVTEPIEFKESKVFGEGSSFV
                     VFGDYETIAPVLEAESEKIIEIEIETAARYSAVPLLDIKKINARIEPGAIIRDQVSIG
                     NNAVIMMGAIINIGAVIGENTMIDMGAVLGGRATVGKNCHIGAGAVLAGVIEPASAKP
                     VIVEDGVLVGANAVIVEGVHIGKDAVVAAGAVVLEDVAAETVVGGIPARVLKISDDQT
                     KENTALIAALREL"
     gene            6749..7750
                     /locus_tag="IXT20_002255"
     CDS             6749..7750
                     /locus_tag="IXT20_002255"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HAQ4860685.1"
                     /translation="MREIDTCIVGATGLVGQTMLKVLEEYDFPVGRLKLLASKRSAGK
                     VCVFKKKKHTVQELTETSFEGYDLALFSAGASVSKEFAPIARSQGLFVIDNSSAWRED
                     SDIPLIVPEVNLEDYTLNRLIANPNCSTIQAVLPLKALQEAFGIQRVNYSTYQAVSGS
                     GQKGIEDLHRTQNDEEPVLYPYNISQTVIPEIDQPLENGYTKEEQKMINETRKILHQP
                     ALPVSATCVRVPIENGHGVSVAVQLQKEFTTEEIRECLSQFPGIQLEDDLAAHKYPTS
                     ILARGTDMVYVGRIRKDTSLENGLLFYTTADNIRKGAAANAVQIAAALMQKIAEVAR"
     gene            7747..9108
                     /locus_tag="IXT20_002256"
     CDS             7747..9108
                     /locus_tag="IXT20_002256"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HAQ4860686.1"
                     /translation="MSNTLKVAKFGGTSMSHEEQFKKVRTIVLSDPNRQVIVVSALGK
                     RTSDDDKVTDLLYLIYAYLQHHVDWTPLWKRICDRFIQVRDVLALQLPIEDELARIQK
                     QLEDEKITEDYLVSRGEYLTAQLMAEYLGYQFIDAKDLIFFDYDGKIDQEKTAQEITK
                     AFKRYPKMVVPGFYGANPAGKEKLLGRGGSDITGAILAKMLQAESYENWTDVSGIMMA
                     DPRIIDHPKIIEEISFRELREMAYMGANVLHEAAVFPVQEADIPIQIKNTNDPFAQGT
                     KISNHEIEKENGLTGIAGRKDFLSITLFKRHMSDEIGFIWKAMSIFAKHEISIEHIPS
                     GIDNIGVIVSAEAIADKLFLITKELKETLGVEEIVVIEDLALISVVGGPHKELIGLSG
                     KVLSILNKLEIRTSILSQGAQELNLIIGVPNNQYETVVKGIYEGMVNTDAERISNGTP
                     SNS"
     gene            9068..10219
                     /locus_tag="IXT20_002257"
     CDS             9068..10219
                     /locus_tag="IXT20_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HAQ4860687.1"
                     /translation="MLKEYRMALHQIPEIGFHEFKTKKYLYEQVKNSEAVIHEIGETG
                     LLLYFDNQQEETIAFRTDIDALPVKEETNLSFASKHEGYMHACGHDGHMAMLLGLTDF
                     ITKHIEDLIYNVVLIYQPSEEISGGAQSVIQSGLLDKYEVQAIFGFHLWPGLAKGEVF
                     SRPGPLMAQSSETDIIIKGQSAHIASSEKGIDSLEAAVRFLKNVYDFDRQLPAEWNHL
                     MKFGQIQGGTIRNVLAGEVIVSGSIRSYSSDMQNYLKRGLDHLAEQFHSTATISFRYN
                     DGYPAVINHRGLYAALLDSNKLHELDAPVLQAEDFGIYTQQYPCVFFFLGVGDTPALH
                     DPTFDFDMDVLENGVEWYKTILFTENLLVKIDSSSKEERVNNDEFIQSI"
     gene            10194..11363
                     /locus_tag="IXT20_002258"
     CDS             10194..11363
                     /locus_tag="IXT20_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAQ4860688.1"
                     /translation="MMNLSNRFSPRLSRIEVSKIRQFDQQISSIPDVIKLTLGEPDFP
                     TPEHVKQAGIAAIEEDFSHYTGMRGLEELREAACIFQQQRYGLTYDPQTEVLTTVGAT
                     EAIASALLSVLEEGDKVLIPAPAYSGYQPLVELAGAELIPIDTSDTGFVCQPEQFERA
                     FEQYGSAVKAVILNYPNNPTGTTLSAKQLKAIAEVLKKYPVFVISDEVYAELTYSGTH
                     MSIATYLPEQTIVISGLSKSHAMTGWRVGFIFAQKALIDELIKVHQYLVTAATTMSQK
                     AAAEALINGVKDSENMKEQYQTRRDYLVQQLAPLGFEVTQPNGAFYLFCKLPAAIQTD
                     SWQFCVELAEQAKVACIPGIAFGPEGEGYIRISYASSMEKLHEACKRIKDFLTEQ"
     gene            11538..12917
                     /locus_tag="IXT20_002259"
     CDS             11538..12917
                     /locus_tag="IXT20_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAQ4860689.1"
                     /translation="MEKIKVVIVGASHGGHQSILELLTRYGENVDITLFEAGDYISFM
                     SCGMELYLEDKVTDVNDVRNFRPENFPQSNVHILNNHEVKTIDADKKTVTVTSASDNE
                     TKEYPYDKLILSSGVKPNNLPVPGADLENVYLMRGYNWATKIKEKLNDPAIKKIAVVG
                     AGYIGIEAAEASRKAGKEVVLLDVIDRPLGTYLDAEMTDILEQHLNENGIEVLTNAKI
                     EAFTGNGKVEAIKTSEKEIPADLVIQAAGVKANTEWLKGIVDLDERGWIQTNEYLQTN
                     LPDVYAVGDATLAYSIPARKKMPIALATVARREARYVVKHLFEEVPNKPFGGVVGSSA
                     LSVFDYHFAASGLNSFTAAKAGVDVRVSYYEDTLRPKYVPEEQGNPKVSIQLFSDPMT
                     HQLLGGAVLSTYDVTGQGNVLALAIQNKLTLEELAEADFFFQPGFDRQWSMLNLAAQQ
                     ALGEEPFVE"
     gene            13198..13427
                     /locus_tag="IXT20_002260"
                     /pseudo
     CDS             13198..13427
                     /locus_tag="IXT20_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         4482 a         2339 c         2924 g         3682 t
ORIGIN      
        1 gtaaatgttt tttgataaat agcggtctgc tgcgtctgga aaaatcgtaa cgacacgatg
       61 accaggtggt aaacgacgaa tttctcttaa tgctgctgca aaagccgcac cactcgaact
      121 tccaactaat agtccttgtt ctcgtgctaa ctgcctagta tagttaaacc cttctacatc
      181 agagattgtc tcgatctgat tgattgaaag gggagaaaga aacggcggaa tgaattctac
      241 accgataccc tcaatttcgt gaggggctgg atcaccacca tttaacacag agccctctgg
      301 ttctacaccg attattcgaa tgtcgggata tttttctttc aaaaaggtag aagttccagc
      361 aaaggttcca ccactcccaa ttcctgctac aaaactatgg atattttctt tgatttcatg
      421 aaagatttct ggccctaacg tatgatagta agcagcagga ttatctttat tttcaaattg
      481 aagaggcaga tagctgtttg atatctcctc tgcaagtttt ttacttttgc tgatggcacc
      541 gatgattcct tgctcactag gagaatggat gatctttgct cccaatgctt tcatcaattg
      601 ttgtttttct aagctaaact tctctggaac aacaaaaacc gttcttaatt tgtatgtgag
      661 tgcggccaga gcgatcccaa ttcctgtatt tccggcagtt ggttcgatga tcgtcgtttt
      721 ttcattgatt tttccttggg caaatccttc tttgatcaaa taactgccca atctatcttt
      781 gatacttcct ccaggattta aatattctag ttttgcataa atagcagaac ctttaggcat
      841 agaatagtgc tgattgtcaa agcgataaag cggtgtggaa ccaatagctt ctgtaattgt
      901 cgagataatc atgtgggcct cctaaaaaaa gataataaaa aaaggagctg actattaaat
      961 cagctccttg aaagacatag agaaaaattg agtagataaa aagataagtt acctggtact
     1021 cattctatga agggcaatta atagacatga caaatagtat gctgctttta tgcaccatac
     1081 aacagcaacg attcaattgt ttttggttgc tgactgtcat gtgtatccct ccaagtaaaa
     1141 tttttattaa cgatagcatg aaaaaaaata aaagtcaaag gcttgaaaga aaaaaatatt
     1201 tcgatagcat cgtttacccg tgtttatttt tctgacaaat aggcatgaga ccttaaaaga
     1261 tagagaatac tctagttttc tggccggttg tctgatacaa taaagttgat tatgataaag
     1321 aaaagaagga gcaaaaataa tgatctcagc aagtgattta agagccggta tgactttcgt
     1381 acaagacggc aaactgatta aagttctgga tgcaagccac cataaacctg gtaaaggaaa
     1441 tacagttatg cgtatgaaac tgaaagatgt ccgttcaggt gcaacatacg acacaacgtt
     1501 cagaccagaa gaaaaatttg aaaaagcaca tatcgacaca aaacctgtac aatatttgta
     1561 cacgatggat gatacagctt tcttcatgga tttggaaaca tatgagcaat atgaaattcc
     1621 tgtagaaaca gtagaagaag aaatgaaata tatcctagaa aacatggaag taaaaatcca
     1681 atttttcggt agcgaagtca tcggcgttca attaccaaca actgtcgtat tacgtgttgt
     1741 ggaaacacaa ccttccatca aaggtgcaac agtcactggt tcaggtaagc cagcgacaat
     1801 ggaaactggc ttagtagtca atgttcctga cttcatcgaa acagatgaat tgttggaaat
     1861 caatacagca gaaggttctt atgtaaaacg tgcgtcaaaa taattcgcta acggattgat
     1921 aagaatttga aaacagagct tgacctcttg aaaggcagat catcggatct gcctttcaag
     1981 aggttttttg tggaatcagc aaattgaaaa ataaaaaagt atttgatttt atttatattt
     2041 tgaaagatga atggcaaaag ctagagaaag aaaaaactgc acttatttat gaagaaattg
     2101 aaatgattca taaaattgaa aatatccgaa ctattccgaa ctatattgaa gcgaaaatat
     2161 tttttcatca ctaaatagtg ttgaatttat gtttttatgt ataaatagct tctaatagtt
     2221 cgggtatttt gtttttataa ccatttttat tcttattttt aaaaaaatat ttcgaaatat
     2281 actagacaat tcagaaaatt ttgtttaata ttattccaat aaaattcaaa tctttatcaa
     2341 atgaaattcc aataaaaacg gaaaaaaagt gaaaagagga atgaaaaaat gacagaattg
     2401 ataattggcg gagtcacagc aagcacattg gcagaaaagt ataagacacc tctgtatgtg
     2461 tatgatgaag aaaagatgag acaaacgatg ctgtcattca agcgaggctt tgtatcgaaa
     2521 cgttttgata caaaagtgct ttacgcctct aaagcattcc aaacgattga gatgctgcat
     2581 ttagttcgtg aatatggatt cgggctagat gttgtcagtg gtggggaaat ctataccgct
     2641 ttgcaagctg attttcctag agatactatc tacttccatg gtaacaacaa gacgccagag
     2701 gaactgatct atgcacttga aaatgatttg ttgcatattg ttgccgataa tttgatggaa
     2761 gttgaattat tagcagaatt aagccaacgc tatttaaaaa aaatctgtat catgcttcgg
     2821 ttgaatgtcg gagtagaagc acatactcat gaatatatcg tcactgcgca tattgattca
     2881 aaattcggca tgagctatga aagtaaagag tgccaacaag cccttcagtt gataaaagaa
     2941 agcagctatc tagagttaga aggatttcat gctcatatcg ggtcacaaat ttttgatgtt
     3001 actgcttggt tagctgaaat cgacaaattg acgaattact taaccgattt tgaccaacct
     3061 ctttccttga atcttggcgg agggtttggg attcgttata cacaaaatga tcagccgatc
     3121 ccagtcgaag aggcactaaa aaaactgatt gaatatacgg aagaagcttt ggaaaaacga
     3181 aaactgacca ttcgtcaatt actgatcgaa ccgggtagaa gtattgttgg agaagctggt
     3241 acaacactat acacgattgg tttcatcaaa aagacaccac ataaaaaata ttactttgta
     3301 gatggcggga tgacagataa tatccgtcca gctctgtatc aggcagaata cgattgtgat
     3361 ttagcagcaa aactgtcgga agaaaaaaca gagaaagtga cgattgcagg aaaaatgtgt
     3421 gaatcaggcg atgtggtaat taaagaagct tatttgccga aagcagaatc gggtgatctt
     3481 ttagctgttt attccacggg agcttatgga tactcgatga gcagcaacta caatcgagcg
     3541 acacgaccag cagttgtttt tgtcaataat ggccaatcaa gactgattgt tcgcagacaa
     3601 gcattttctg atctactaag gggggaagtt tcctatgatc cttcataaat ataatggctg
     3661 cggaaatgat tttatcttat tagattacgc agaagacaca gattatagtc aattagccgc
     3721 ttctttatgc acaaaagaaa aatatgatat agacggattg atcgccgtaa aaatcgatcc
     3781 gttggaaatg atctattaca acaaagatgg cagccgcgcg ccgatgtgcg gaaatggcat
     3841 tcgctgtttt gctcgatacg taaatgatca gaacatgatg aatgaacagg aattcaacgt
     3901 agagacatta gcaggcacaa gaaaaattac ggtgttggat ccctcacctt tttattgcag
     3961 cgtagatatg ggagtgccag atgattctcc ggaaaaaatc aaagctaatc aaacttatcc
     4021 aataaaaaac caggaaatag agatcaatgg tgaaaaagta acgatcacta gtttattttt
     4081 aggaacgatt cataccgttg tttttgttga aaatgcgcgt gaggaagtct ggagaaaaag
     4141 aggtgaactg atctgtcatc atcctttgtt caaagaaaaa accaatgtca actttgtaga
     4201 agtagtcaat gaacaagaat tgatcgtccg tacctatgaa agaggagtag gttggacact
     4261 tgcttgcggt acaggttgct gtgcctctta tgtggttgct aaggaaactg ggaaaatttc
     4321 agcagatcag gtagttgttc acttggaaca aggagacttg atgatctctg gtgaggatgc
     4381 gatacaaatg gctggcccag cagttcatga atggacaaga atattggagg aatgaagatg
     4441 ataaaaggtt cgatcgtggc gctgatcacg ccgatgaatg aagagggatc agtagattat
     4501 gctggtttgg aaaaattgat ccaattccat ttagatgaac aaacagatgg attattagtt
     4561 ttaggtacaa caggagaatc ttctacgctc acacaatctg aggaggagca gatccttcaa
     4621 ctgacagtaa agaaagtggc tggccgtgtg ccggtgattg caggagctgg aacgaataat
     4681 acgaaagaaa cgattgaaaa agcgaagcat tttgcttctt tgggggcaga cgcacttttg
     4741 gtcatcaccc cttactacaa caaaacaagt gatgcaggac tagctgctca ttttacagca
     4801 attgcagaag cctcaccgat tccgcttatt ttgtataatg ttccttctag aacaggcatg
     4861 tcgattccaa tccatgtatt agtaaattta gcagaacatc ctaatatcat tggtttgaaa
     4921 gaagcctcag gagatatggc ttatgtgatg gatgccgctc ggttgattgg agaagaattc
     4981 ttcctttaca gtggaaatga tgatttgatc ctaccagtaa tgagtgtagg aggaagcggt
     5041 gtaataagtg tatgggcgaa tatccaacct aaaatagtcc atgaattagt aaaagatacg
     5101 caggacggaa gatggcagca agcaaaagaa aagcaattaa atgcacttga actgatccat
     5161 gccttgttca gtgagaccaa tcctattcct gtcaaagcag ccatgtcatt attagactta
     5221 ccttccggac ctctacgcct gcctttagtt tctctttcaa aagagaaaaa gaaacaatta
     5281 gctcagcttt tgttaaaaga aaggacggtt gaaaaatgaa aatcggactg attggcagtg
     5341 gacagatggg aaatagagtc gaagaagttg cacaagaaag aggagatacg gtccttcttt
     5401 tacggacggc acctaaagaa gcaactacgc ttgcttttac ggaactgcca gaactgctta
     5461 ttgatttttc tcatccagat aatttagaga aaatcctttc gtatagctta tcatatcaac
     5521 ttccgttagt cattggcacg acaggttata cacaatcaca gttccaagaa ataaaagaac
     5581 atgccaaaca tgtacctgtg atgtatagtg ctaatttttc gttcggcatc atggtgatga
     5641 atcaactaat caagcaagca gctgcaatgt tgcaaggctg gcagatcgaa ctgattgaaa
     5701 aacatcatag tcgtaaaaaa gatgcaccat ctggtactgc aaatatgctg cttcaaacaa
     5761 tccaagaagt gcaaaagctg cagcccgtat acaactggca agggaaaaaa agagaagaaa
     5821 accaaattgg tgtacacagt attcgtgcag gttctttacc tggtgaacat gatgtattgt
     5881 ttgctgctac tgatgaaata tttacgatca aacatgaatc tttttctaat cgaatctttg
     5941 ctgcaggcgc agtagaagca gcggactggt tgaaagatca gtcaccaggt tattacaaat
     6001 tagaagattt attaatggac aaaggggtgt aaaaaacgtg gaagctcaag aaatcatccg
     6061 ctacattcaa acggcatcta aaaagacgcc tgtgaaagtc tatctgaatg taaccgagcc
     6121 aatcgaattt aaagaaagta aagtttttgg tgaaggaagc agcttcgttg tttttggtga
     6181 ctatgagacg attgctccgg ttttagaagc tgaatcagaa aaaatcattg aaatcgaaat
     6241 cgaaactgct gcaagataca gcgcagttcc tttgctagat attaaaaaaa ttaatgctcg
     6301 tatcgaacca ggtgctatta tcagagatca agtgtcaatc ggcaacaatg ccgtgatcat
     6361 gatgggagcg attatcaata ttggtgcagt aatcggtgaa aacacgatga ttgatatggg
     6421 tgctgtctta ggtggacgag cgacagtggg caaaaactgc catattggtg caggagctgt
     6481 cttagcaggt gtgatcgaac cagcatcggc caaaccggtt atcgtagaag acggcgtact
     6541 ggtaggtgcg aatgcagtga ttgttgaagg tgtccatatc ggaaaagatg cagtcgtggc
     6601 agcaggagca gtggttttag aagatgtggc agcagaaact gttgtcggtg gaatacctgc
     6661 acgtgtatta aaaataagtg atgatcagac aaaagaaaat acagcattga ttgctgcgtt
     6721 acgcgaatta tagaggagat ggagaaaaat gcgcgaaata gatacctgta ttgtgggggc
     6781 aaccggatta gtcggccaaa cgatgctcaa agtgttagaa gaatatgatt ttccagtagg
     6841 acgattgaaa ttattagctt caaaacgttc agccggtaaa gtttgtgtat tcaagaaaaa
     6901 gaaacacact gtacaggaat tgacagaaac ttcatttgaa ggatatgatt tagcgctatt
     6961 ttctgcaggt gcttctgtat ccaaagagtt tgcgccaatc gcacgaagtc agggactatt
     7021 tgtcatagat aattcctcag catggcggga agattcggat attccattaa tcgttccaga
     7081 ggtcaatctg gaagactata cattgaatcg gctgattgca aatccaaatt gttcaaccat
     7141 tcaagcggta cttccgctta aagctttaca agaagccttt ggtatccagc gagtcaatta
     7201 ttcgacctat caagcagttt ctggttccgg gcaaaaaggc attgaagatc ttcatcgtac
     7261 gcaaaatgac gaggaaccag ttttgtatcc ttataatatt agccaaacgg tgattccgga
     7321 aatcgatcag cctttggaaa atgggtatac aaaagaagaa cagaaaatga taaatgagac
     7381 ccgtaagatt ctgcatcagc ctgctttgcc agtatcagca acctgcgtac gtgtgccaat
     7441 cgaaaatgga catggagtct ctgtggcagt tcaactgcaa aaagagttta cgacggaaga
     7501 aattagagag tgtctcagcc agtttccagg gattcaatta gaagatgatc tagcagcaca
     7561 taaatatccg acgagcattc ttgctagagg aacagatatg gtttatgttg ggaggatcag
     7621 aaaagacact tctttagaga acggattgtt gttctatacg actgccgata acatacgcaa
     7681 aggagccgct gcgaatgccg tacagattgc tgcagcttta atgcagaaaa tagcggaggt
     7741 ggcaagatga gtaatacgct gaaagtagcg aaattcggcg gaacatcgat gtctcatgaa
     7801 gagcaattca aaaaagtccg tacaattgtt ctttcagatc ccaatcgcca agtgatcgtt
     7861 gtttctgctt taggcaaacg tacgtcagat gatgacaaag taacagactt actttatttg
     7921 atttatgctt accttcagca tcatgttgat tggacacccc tttggaaaag gatttgtgat
     7981 cgtttcattc aagtaagaga tgttcttgcc ctgcagttac ctattgaaga tgaactagca
     8041 cgcatacaga aacaactgga agacgaaaag ataactgaag attatttagt aagtagaggt
     8101 gaatatctga ctgctcaact gatggctgag tatcttggtt accagtttat tgacgcaaaa
     8161 gacctgattt tctttgacta tgatgggaaa atcgatcagg aaaaaacagc acaggaaatc
     8221 acaaaagcat ttaaaagata tccgaaaatg gttgttccag ggttttacgg ggcgaaccct
     8281 gcaggaaaag aaaaattatt gggccgtggg ggaagcgaca ttaccggcgc gatactggcg
     8341 aagatgttgc aagcggaaag ttatgagaat tggacggatg tttcaggaat catgatggct
     8401 gatccgcgaa tcatcgatca tcctaaaatc atcgaggaaa tctcttttag agaactgaga
     8461 gagatggctt atatgggggc taacgttctt catgaagcag ctgttttccc agttcaagag
     8521 gctgatatcc ccatccaaat aaaaaatacc aatgatcctt tcgctcaagg gacaaaaata
     8581 tcgaatcacg aaatagaaaa ggaaaatgga ctgacaggaa tagctggacg caaggatttt
     8641 ctttccatca ctttattcaa acgtcatatg tccgatgaaa tcgggtttat ctggaaagca
     8701 atgagcattt ttgctaaaca tgagatatca atcgaacata ttccttcagg gatcgacaat
     8761 atcggagtga tcgtctcagc agaagcgatt gccgataagt tatttctcat cacgaaagaa
     8821 ttaaaagaaa cacttggcgt ggaagaaatc gtcgtgatcg aagacttagc tttgatctct
     8881 gtcgttgggg gtccgcataa agaattgatt ggattatctg ggaaagtgtt atcgatattg
     8941 aacaaattag aaattcgcac atcgatctta tcacaaggag cacaggagtt gaatctgatc
     9001 atcggtgtac cgaataatca atatgaaaca gtagtgaagg gtatctatga agggatggtg
     9061 aatacggatg ctgaaagaat atcgaatggc actccatcaa attcctgaaa tcgggtttca
     9121 tgaattcaag acaaaaaaat atttatatga acaagtaaaa aacagcgagg ccgttatcca
     9181 tgaaatcggc gaaacaggac tactccttta ttttgataat cagcaagaag aaacgattgc
     9241 ttttcgaaca gatatcgatg cactgccagt caaagaagaa acgaatctct cttttgcttc
     9301 taagcatgaa ggttatatgc atgcatgtgg tcatgacgga catatggcga tgcttttagg
     9361 gttgactgac tttataacta aacacattga ggatcttatt tataatgtag tactgatcta
     9421 tcagccatca gaagaaattt ccggtggcgc acaaagcgtt attcagtcag gattgttgga
     9481 taaatatgaa gtacaagcaa tatttggctt tcatctatgg cctggactag ctaagggaga
     9541 agttttctct cgaccaggac ctttgatggc acaaagcagt gaaacagaca ttatcattaa
     9601 aggccagtct gcacatattg cttctagtga aaaaggaatc gatagtctgg aggcagctgt
     9661 acgatttttg aagaatgttt atgattttga ccggcaatta ccggcagagt ggaatcatct
     9721 aatgaaattt ggccaaatcc aaggtggaac gatccgaaat gttttagctg gagaagttat
     9781 tgtatccgga agtatccgtt cttacagttc agatatgcaa aattatttga agaggggatt
     9841 agaccattta gcagaacagt ttcactctac agctactatt tcttttcgct ataatgacgg
     9901 ctatccagct gtaatcaacc atagaggctt atatgcagca ctgttagatt caaacaaact
     9961 acatgaatta gatgctccag ttcttcaagc cgaagatttt ggcatatata cgcaacaata
    10021 tccatgtgta tttttctttt taggtgtcgg tgatacccct gctttacatg atccgacgtt
    10081 cgattttgat atggatgttt tagaaaatgg tgtagaatgg tacaaaacga ttctttttac
    10141 agaaaatctt cttgtaaaaa tcgatagcag ttcaaaagag gagcgagtaa ataatgatga
    10201 atttatccaa tcgatttagt cctaggcttt ctagaataga agtatcaaaa atacgacaat
    10261 tcgatcagca aatatcgtcg ataccagatg tgatcaaact caccctggga gaaccggatt
    10321 ttccaactcc tgaacatgtc aagcaagcag gaatagcagc gattgaagaa gacttttctc
    10381 actataccgg aatgagagga ttagaggaac tgcgggaagc ggcttgtata tttcagcaac
    10441 agcgttatgg attaacttat gatccgcaaa cggaagtttt gacgacagtt ggtgcaacgg
    10501 aagcgattgc ctctgcgttg ttatctgttt tggaagaagg agacaaggtt cttattccag
    10561 cgcctgctta ttcaggatat cagccattag tggaactagc tggtgctgaa ttgatcccga
    10621 tagatacttc tgacacaggg tttgtctgtc agccggaaca atttgaacga gcatttgaac
    10681 aatatggatc agctgtcaaa gcagtcattt taaactatcc taacaatcct actgggacga
    10741 cattgtctgc aaaacaattg aaagcaatcg ctgaggtttt aaaaaaatat ccggtatttg
    10801 ttataagtga tgaagtatat gctgaattga cttatagtgg aacgcatatg tccatagcta
    10861 cttatcttcc agaacaaaca atcgtgataa gcgggttatc caagtcacat gcgatgactg
    10921 gttggcgagt gggctttatt tttgctcaaa aagcccttat tgacgaacta attaaagtcc
    10981 atcaatatct ggtgactgcc gctacaacga tgtcgcaaaa agctgcggct gaagcgctaa
    11041 taaatggggt gaaagatagc gagaatatga aagagcaata tcaaacacga agagactatc
    11101 tggtccagca attggcgcct ttaggatttg aagtgaccca gccaaacggt gcattttact
    11161 tgttttgcaa actcccagct gccattcaaa ctgactcttg gcagttttgt gtagaattag
    11221 cggaacaggc aaaagttgcg tgtatcccag gaattgcatt tggcccagaa ggagaaggtt
    11281 atatccggat ttcttatgct agcagcatgg aaaaattaca tgaggcctgc aaacggatca
    11341 aagacttttt aacagaacag tgattcatag attttatcaa aaaaacaatc gttaattttg
    11401 cggtatattc tgcaaggaga cgattgtttt ttaaatttag ttcaaatgaa cgcgtttctt
    11461 ttgattcata actaatgtaa tgttaagttt aaattgttta gaatgatttt aaaagaatat
    11521 tcaaggaggt ttcatcaatg gaaaaaatca aagttgtcat tgtaggtgca tctcatggag
    11581 ggcatcaatc gatcttggaa ctattgacac gatacggtga aaatgtagat atcacgctat
    11641 ttgaagcagg agactatatt tccttcatgt catgtggtat ggagctgtat ttagaagaca
    11701 aggtaacaga tgtaaatgat gtacgtaatt tcagaccaga aaactttccg caatccaatg
    11761 ttcatatttt aaataatcac gaagtaaaaa cgatcgatgc cgataaaaaa acagtaacgg
    11821 ttactagcgc atcagataac gaaacaaaag aatatcctta tgacaaattg attttgagtt
    11881 ctggtgtcaa accaaacaat ctgccagtac caggagcaga tctagaaaat gtctacttga
    11941 tgcgtggata caattgggca accaaaatca aagaaaaatt aaatgatccg gctattaaaa
    12001 aaatcgctgt cgtaggtgcg ggttacattg ggatcgaagc agcagaagca agccgaaaag
    12061 ctgggaaaga agtcgtattg cttgatgtga tcgatagacc tttaggcact tatttagatg
    12121 ctgagatgac agatatttta gaacaacacc taaatgaaaa cggtattgaa gttttgacaa
    12181 atgcaaaaat cgaagctttt actggtaacg gaaaagttga ggcaatcaaa acaagtgaaa
    12241 aagaaatccc agcagatcta gtcattcaag ctgccggtgt gaaggcaaat acagaatggt
    12301 taaaagggat cgtcgactta gatgaacgtg gctggatcca aacaaatgaa tacttgcaaa
    12361 cgaatcttcc agatgtgtat gcagtaggag atgctacact tgcttattct atcccagcac
    12421 gcaaaaaaat gccgatcgct ttagcgactg ttgcacgtag agaagcacgt tatgtagtaa
    12481 aacatctttt tgaagaagta ccaaacaaac cgttcggcgg agtcgtaggt tcctctgcat
    12541 taagtgtctt tgactatcat tttgcagcaa gcggattgaa tagctttaca gcagcaaaag
    12601 caggagtaga tgtacgagtg tcttattatg aagatacgtt acgaccaaaa tatgtaccag
    12661 aagaacaagg aaatccaaaa gtgtccatcc aattattctc ggatccaatg acccatcaat
    12721 tgctaggagg agcagtatta tctacctatg atgttacagg tcaaggaaat gtattggcac
    12781 tagcgatcca aaacaaactg actttagaag aactagcaga agctgacttc ttcttccagc
    12841 caggctttga ccgacaatgg agcatgttga atctagccgc tcaacaagcg ttaggagaag
    12901 aaccatttgt agaataagaa aacgaaatat ttgaaaagag gttgtgaaaa agctgccttg
    12961 catcaagtaa taagaacagc caagtaaaaa cagcttctta tgtttttaac ttggctggac
    13021 ttattaccgc agatgcaagc ttttgaacac cgtttatttt caaaaaaggg actgtgacac
    13081 gatttgtgta ccgaccccca aaagttagac caaaaaatct aacttttgga ggtcggtatt
    13141 ttttatggtt aaatatactt ttgaacttaa attaaagatc gttcatgatt atttggatgg
    13201 caaaggcgat tggaccgtaa aaatccagat caagagattg aagcggaaat gcttaaaatt
    13261 cgggagaagc acaaggatta tggctgttta cggatgacca atgaactaag aaaccgcggt
    13321 ttctctatca ataaaaagaa agttcagcgc ttgatcaaga agctaggaat aaaagtaacg
    13381 gcatacacgc gtaaatcacg tcgctacaat tcttatcgag gaaaagt
//
LOCUS       DACGLE010000075        13156 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0534
            SAMEA3304449-rid9560453.denovo.079, whole genome shotgun sequence.
ACCESSION   DACGLE010000075 DACGLE010000000
VERSION     DACGLE010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304449
            Sequence Read Archive: ERR985064
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13156)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13156)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702225.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702225.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 20:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,045
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 27 of 67
            Pseudo Genes (incomplete)         :: 25 of 67
            Pseudo Genes (internal stop)      :: 19 of 67
            Pseudo Genes (multiple problems)  :: 4 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13156
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304449-rid9560453.denovo.079"
                     /strain="VREN0534"
                     /db_xref="taxon:1352"
     gene            <1..942
                     /locus_tag="IXT20_002261"
     CDS             <1..942
                     /locus_tag="IXT20_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAQ4860690.1"
                     /translation="NRSNADKYNPEGQKVTTDLNKEPDASEGIKNKEDLPKGTTYAWK
                     EKVDVSTAGNKKGTIIVTYPDGSSDEVEVDVTVTDTRSDADKYNPEGQKVTTDLNKEP
                     DASEGIKNKEDLPKGTTYTWKEKVDVSTAGNKKGTVVVTYPDGSKEEVEVTISVVDKK
                     APNKPQVDPITEGDQIVTGKTEPNAEVTVTLPDGSQYHGTADENGNFTVKVPKLEAGT
                     KVIVTATDESGNTSEPTDVVVSSNTKDSGKNDNKGKTDNQGSNSNQDKNRGKSQSSKV
                     FPKTGEIDSNIFTISGGLILLGTLGLLGYKNRKKENE"
     gene            1083..2426
                     /locus_tag="IXT20_002262"
     CDS             1083..2426
                     /locus_tag="IXT20_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAQ4860691.1"
                     /translation="MEKIVIIGSNHSGLAAAQFLNLSDSNYEVILIDKKSEISYLECG
                     TALFIKREVSNLKDLFYQNRRELNSKIKKLFLETVADKIDIQNKKIICVKKDGTRFSQ
                     EYDKVILATGSKQIMLDIPGNNLANIFSIKNFPNAKEIFKALDDSTIKKVGIIGAGYI
                     GVEIAEAIRKRGKEVYLFDVADRVLSTYYDRSFSDKVEEILSKNGIHLCLSESPKKYL
                     GKEKIEKIVTTRDNKYAMDMVIQAIGFLSHSPIGKKEFLRDVSGAYLTDEYQQTNIKD
                     IYAIGDCATTFFTSINRSSVDFSISNALRTAYIATQHINNQNFTPSGSQFTNGFSIFG
                     HNFYAAGLNLKTARLLNKSIDYIEIEDYIRPIFSKKNSIVKLRISFEKKAKRIVGVQF
                     YSQEDLSGLLTMFSLAIEEQITLDKLKMLDYLFHPSFSQPYNFILKAVTGLNSGA"
     gene            2537..3988
                     /locus_tag="IXT20_002263"
     CDS             2537..3988
                     /locus_tag="IXT20_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HAQ4860692.1"
                     /translation="MKKSLKLASAVLLMSHLLIATPFHVLASENSTYKIAKERTKTTE
                     MSNSDVTAESSELLTTSSSESSTTLNAESSTTSSSESSMTSNAESSTTSSSESSMTSN
                     AESSTTSSSESSTISSTESSTTSSSTSEITSSKVTKPNHIESSKKEAPKTEGQTKTFS
                     TSQQIESTETFQSQPVLSGNEEIHFDKNESVENFIMKIGDSARKIGQKYDLYASVMIA
                     QAILESASGQSQLAQAPNYNLFGKKGTYNGSFVIMVTNEDLGNGTLYKTQSKFRVYEN
                     YEESFEDYAKLLTKGISGNKDFYAGALKANSKTYREATKFLTGRYATDTQYYLKLNGL
                     IKTYDLTDYAKEVQGPQMNKNGYQIPIHNYSISSLFGNRGDEFHRGLDMAAAQGEPIH
                     AVKAGKVIKAEFHYSWGNYVVIQHEDGSTALYAHQQQYLVKTGDNVVQGQMIGYVGST
                     GNSTGPHLHLEICKDSSLSQSMLVDPKTLLFGN"
     gene            4406..4621
                     /locus_tag="IXT20_002264"
     CDS             4406..4621
                     /locus_tag="IXT20_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4860693.1"
                     /translation="MNQGIADIKLIKEVLQESTANAVASGSGISLSTAKKLKSGERNV
                     EKLNLSDAIKITEFAIKNRHVKIEIWK"
     gene            complement(4707..5618)
                     /locus_tag="IXT20_002265"
     CDS             complement(4707..5618)
                     /locus_tag="IXT20_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAQ4860694.1"
                     /translation="MKIKIERNQKEKNQKKNKERVISVGKHRKMVLALWLLLSCSLSF
                     GIYRNFTAIDQHTTHEKVVIKEKVVNTSGVESFTEDFAKEYFSWKNNKEAIEKRMSNL
                     EQYLTENGLALSQDMVRADIPTSSEVQSIKILDVEKHSEEFIVSFLVEQQITEGKKTQ
                     SISSAYRVTIFEDENGSHIVTSLPTMIAKPEKAKYESKQIGIESDIDAKTTEEVTDFL
                     ETFFKLYPSASEKELEYYVENKAVQPINSNLKLVELVNPTFKLKGKEIQGEIFVKYLN
                     NTTKVSNIFQFTLTLQKVKNWKIIDIG"
     gene            complement(5637..6641)
                     /locus_tag="IXT20_002266"
     CDS             complement(5637..6641)
                     /locus_tag="IXT20_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase P60"
                     /protein_id="HAQ4860695.1"
                     /translation="MNVKKLSIISLTILTLSFMVVLMCVGLLFGEDSEGSNSSGASSG
                     GSNVSEEVLKHRAMVEKYAKESNISEYVLILLAIIQVESGGTMEDVMQSSESAGLPPN
                     SLSTEASIKQGCLYFASLVKRSKELGCDQDSIIQAYNYGGGYLDHVTKNGKKHNFALA
                     ESFSKEKSSGQKVDYPNPIAIKKNGGWRYNYGNMFYCLLVKQYLTTTQFDDKTVQGIF
                     DEAFKYEGTAYVFGGASPETGFDCSGLTQWCYAKVGLKLPRVAQDQYNAMTHIDVKDA
                     KPGDLVFFHSTYDAGTYVTHVGIYAGENRMFHAGDPVGWTNLTESYWQQHLIGAGRYK
                     "
     gene            complement(6638..8761)
                     /locus_tag="IXT20_002267"
     CDS             complement(6638..8761)
                     /locus_tag="IXT20_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAQ4860696.1"
                     /translation="MKKKLLRIVGIIAISLCTILLLLSLIGTLAEATGLVDDTVKSGN
                     LYSQYSLDNYQLDFFVDSSWGWLPWNWGDGLGKSVMYGLYAITNFIWTVSLYLSNATG
                     YVVQEAYKLDFISDTAESIGKNIQTLAGITENGLQVSGFYFGFLLLMILALGVYVAYI
                     GLLKREATKAVRAVINFVTIFLLSGSFIAYAPTYITKINNFSSDVSEAALSLGTELVV
                     PNSESKGKGSVDLIRDSLFSIQVRQPWLLLQFDDSNIEEIGEDRVNKILSVSPDGNKG
                     KDREEAVKAEIEDNDNANLSITKTMNRLGTVVFLVLFNIGISFFVFLLTGIMLFSQIL
                     FIIFAMFLPISFLLSMLPTYESLGKKAIIRLFNTIMMRAGVTLVITTAFSISTMFFNI
                     SATYPFFMVAFLQIVTFAGIYFKLGDIMSMFNLQSNDSQSMGRRIMRKPHMLMNRKLR
                     QLNRNVIRTLAFGGGAAVGHKLAKEQSKSKFKPSGSSLRKNSRLPNDHEVPSESTKEN
                     PVSNNKKQSRMNLAGRKMGKVLDTQALVKDKTKQVRDQVRNTPTNLKYNFHKGIEKTK
                     KAPKEFKRGLVQEKANRAELREKQRQHRDEKMAEKRKVLNETGNRHKKRRENVSIKPE
                     RQPQKDRTPKKEAPKQIVRVNPNPEIKRKLTSHEIVSKRISQPSSKKNSFQQVKQRST
                     LPKRTNQKVQKRMNPRPKSRSGEKK"
     gene            complement(8766..11213)
                     /locus_tag="IXT20_002268"
     CDS             complement(8766..11213)
                     /locus_tag="IXT20_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAQ4860697.1"
                     /translation="MYPIKYIENNLVFNQDSECFAYYELVPYNYSFLSPEQKYQVHDN
                     FRQLIAQNREGRIHALQIATESSIRATQERSKKEITGRLKEVAEQRIDLQTDALVSMI
                     GDSQIDYRFFIGFKLIVTDEEVNLKSLKNSFLSGLQEFVYGVNHHLMGDFVSLSNEEI
                     RRYTKLEKLMESKLARRFKVRRVTPSDLTYLIEHIYGEKGTPFEEYEFQLPKRKLKSE
                     TLIKRYDLLRPSRCLIEEKPRYLRMEHENHESYVAYLTINTIVGEMEFPSSELFYYQQ
                     QQFTFPIDTSMNVEIVTNKKALATVRNKKKELKDLDNHAYQSDNETNSNVLDALDSVD
                     ELETMLDQSKESMYKLSYVVRVSAESLDELKRRCDEVFDFYDDTNVKLVRPFGDMMGL
                     HEEFLPSSKRYMNDYIQYVTSDFLAGLGFGATQMLGELEGIYFGYNVDTGRNVYLKPA
                     LASQGVKGSITNALAAAFLGSLGGGKSFSNNLLVYYAVLFGGQAVIVDPKGERGGWKE
                     TLPEITDEINILNLTSKPENQGLLDPYVIMKKKKDSESLAIDILTFLTGISSRDGEKF
                     PVLRKAIRTVTQSEQRGLLQVINELRKEGSPVAENIADHIESFTDYDFAHLLFSDGSI
                     TQSISLDKQLNIIQVADLVLPDAETTFEEYTTMELLSVSMLIVISTFALDFIHSNREI
                     FKIVDLDEAWSFLQVAQGKTLSNKLVRAGRAMNAGVYFVTQNSDDLLNERLKNNIGLK
                     FAFRSTDIHEIKKTLEFFGLDQEDESNQKRLRDLENGQCLMQDLYGRVGVVQVHPVFE
                     ELFHAFDTRPPVQKRME"
     gene            complement(11200..11589)
                     /locus_tag="IXT20_002269"
     CDS             complement(11200..11589)
                     /locus_tag="IXT20_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAQ4860698.1"
                     /translation="MKKIKSYTSIWAVEKVIYAINDFQLPFPVTFNQMAWFVLSLLFV
                     IVFAHVPPLSMIEGAFLKYLGIPVAVAWFMSQKTFDGKKPLGFLRSFISYHLRFKVTF
                     AGKKVKEQKKRWNEAITLVRSVNDVPD"
     gene            complement(11672..12396)
                     /locus_tag="IXT20_002270"
                     /pseudo
     CDS             complement(11672..12396)
                     /locus_tag="IXT20_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407828.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(12445..12948)
                     /locus_tag="IXT20_002271"
     CDS             complement(12445..12948)
                     /locus_tag="IXT20_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HAQ4860699.1"
                     /translation="MEQMRVYIANLGKYNEGELVGAWFSPPVDFDEVKERIGLNDEYE
                     EYAIHDYELPFEIDEYTPIEEINRLCGLAEELEGTPIGEVALEIQHAFFNSFEEMVEH
                     VDDIICYPDCNDMSDVAYYLIEETGALGEVPTHLQNYIDYEAYGRDLELEGNYLVTNR
                     GTFEFIG"
     gene            complement(12960..13121)
                     /locus_tag="IXT20_002272"
     CDS             complement(12960..13121)
                     /locus_tag="IXT20_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002392227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4860700.1"
                     /translation="MAIVVIGLFLGFKREFSKLIGFLVIALIAVGLVFNASGVKDVLL
                     NLFNRIVGA"
BASE COUNT         4335 a         2473 c         2229 g         4119 t
ORIGIN      
        1 aatcgttcta atgcagataa atacaatccc gaaggacaaa aagtgactac agacttaaac
       61 aaagagccag atgcatctga gggaattaag aacaaagaag atttaccaaa aggaactact
      121 tatgcttgga aagagaaagt agatgttagc acagctggaa acaaaaaagg cactattata
      181 gtgacatatc cagatggatc atctgatgaa gttgaagtag acgttacagt gacagatact
      241 cgttccgatg cagataaata caatcctgaa ggacaaaaag tgactacaga cttaaataaa
      301 gagccagatg catctgaggg aattaagaac aaagaagatt taccaaaagg aactacttat
      361 acttggaaag aaaaagtaga tgttagtaca gctggaaaca aaaaaggcac tgttgtagta
      421 acatatccag atggatctaa agaagaagta gaagttacta tttctgtagt agataaaaaa
      481 gcaccgaata aacctcaagt tgatcctatt acagagggtg accaaattgt tactggtaaa
      541 actgaaccaa atgcagaggt aacagttaca ttaccagacg gaagtcaata ccatgggaca
      601 gctgatgaga atggtaattt tacagttaaa gttcctaaat tagaggcagg aacaaaagtg
      661 atagtaactg caactgatga atctggtaat actagtgaac ctactgatgt agttgtttct
      721 tccaacacaa aagacagtgg aaaaaacgat aataaaggta agactgataa tcaaggtagc
      781 aatagcaatc aagacaaaaa tagaggcaag agccagtcct caaaagtttt tcctaagacg
      841 ggagaaattg acagtaatat ctttactatt agcggtggtt taatattgtt aggaacttta
      901 ggattattag ggtataaaaa tcgaaaaaaa gagaatgaat agaaaatcaa tttcttaagt
      961 cttctctgag aagaggaaga atctttttct tcctcttttt gtttttttat gatgagaaat
     1021 aagtgttagt taaagatgag tatacttttt atagattata gaagaaaaaa gaaagtgatt
     1081 gagtggaaaa aatagtaatt attggttcaa accattcggg gctagccgca gctcagttcc
     1141 taaacttaag tgattcaaat tatgaagtta ttctgataga taaaaaatca gaaattagtt
     1201 acttagaatg tgggacagcg ttgttcataa aaagagaagt tagcaattta aaagatcttt
     1261 tttatcaaaa tagaagagaa ctaaattcta agataaaaaa gttgtttttg gagacagtag
     1321 ctgacaaaat tgatattcaa aataaaaaga ttatttgtgt aaaaaaagat ggtacaagat
     1381 tctctcaaga atatgacaaa gttatattag ctacaggttc aaagcaaatt atgttagata
     1441 taccggggaa taatttagct aatatttttt cgataaagaa ttttccaaat gcaaaagaga
     1501 tttttaaagc tttggatgat agtactatta aaaaagtagg aattattgga gcaggatata
     1561 ttggagttga aattgctgaa gctataagaa aaagagggaa agaagtgtat cttttcgatg
     1621 ttgcagatag agtgttgtcg acctattacg accgtagttt ttcagataaa gttgaagaaa
     1681 tactatcaaa aaacgggata catctttgtt tatcagaaag tcctaaaaag tatttaggaa
     1741 aagaaaagat tgaaaaaata gtgactacaa gagataacaa atacgctatg gatatggtca
     1801 tacaagctat aggattccta tctcatagtc caatagggaa aaaggagttc ttgagagatg
     1861 ttagtggagc atatctaacg gatgaatacc aacaaacaaa tataaaagac atatatgcta
     1921 ttggagattg tgctacaact ttttttacat caattaatag aagtagcgtt gatttttcta
     1981 tctctaatgc tctaagaacg gcttatattg caactcaaca tattaacaat caaaatttta
     2041 caccttctgg ttcccaattc acaaatggat tttctatatt tggtcacaat ttttatgcag
     2101 caggtttgaa tttaaaaaca gcacgcttat taaataagtc tatagattat atagaaatag
     2161 aagattatat tagaccgatt ttttctaaaa aaaacagtat tgtaaaatta agaattagtt
     2221 ttgaaaaaaa agcaaagaga atagtaggtg tacaatttta tagtcaagaa gatctatctg
     2281 gattattgac aatgttctct cttgctatag aagagcaaat tacattagac aaattgaaaa
     2341 tgttagatta tttatttcat cctagtttta gtcaacccta taattttatt ttaaaggcag
     2401 taacaggatt aaatagtgga gcataatcgt tactaaatga aatcaagaaa tatactgtat
     2461 tactcaattc attgaaaata tttgtcatat tttttaatag aaaacactat taaagaaaaa
     2521 aagagaggta aatcaaatga aaaaatcttt aaaacttgca tcagcagttc tattaatgtc
     2581 ccatctcctt atagcaacac ctttccatgt gttggcaagc gaaaattcaa catacaaaat
     2641 tgcaaaagaa agaaccaaga cgactgaaat gagtaattcc gatgtaacag cagagagtag
     2701 tgaacttttg acaacttcat caagtgaaag tagtacgact ttaaatgccg agtcttcaac
     2761 aacttcatca agtgaaagta gtatgacttc aaatgccgag tcttcaacaa cttcatcaag
     2821 tgaaagtagt atgacttcaa atgccgagtc ttcaacaact tcatcaagtg aaagtagtac
     2881 gatttcaagt accgaatctt caacaacttc aagtagtaca agtgaaataa cttcttcgaa
     2941 agttacaaag ccaaaccata tagagtcttc aaaaaaggaa gcgccaaaaa cggaaggaca
     3001 aacaaaaact ttttcgacat cacagcaaat agaatcaaca gaaactttcc aatcacaacc
     3061 agtactatct ggtaatgaag aaattcattt tgacaaaaat gagtctgtag aaaattttat
     3121 tatgaaaatt ggtgattctg cgagaaaaat tggtcagaaa tatgatttat atgcttctgt
     3181 tatgattgca caagcaatct tagaatctgc cagtggtcaa agccaattag ctcaggcacc
     3241 gaattacaat ttatttggga aaaaaggaac ttataatgga agctttgtca taatggtaac
     3301 aaacgaagat ttgggtaatg gaacgcttta taagactcaa tcgaaattta gagtgtatga
     3361 aaactatgag gaaagttttg aagattatgc caaattatta actaagggaa ttagtggaaa
     3421 taaagatttt tatgccggag cattaaaagc taatagtaaa acatatcgag aagcaactaa
     3481 attccttaca ggacggtatg caacagatac tcaatactat ttaaagctaa atggattaat
     3541 taaaacctat gatttaactg actatgccaa agaagttcaa ggtccacaaa tgaataagaa
     3601 cggatatcag atacctattc ataattattc aatatctagt ttgtttggaa atcgtggaga
     3661 tgagttccat cgaggacttg atatggcagc tgcacaaggt gaaccgattc atgcagttaa
     3721 agctggtaaa gttattaaag cagagtttca ctattcttgg ggaaattacg ttgttataca
     3781 acacgaagat ggatcaacag cgttatatgc tcaccaacaa caatacttag taaaaactgg
     3841 agacaacgtt gtgcaaggac aaatgattgg ctatgtagga agtactggga atagtacagg
     3901 accacacctc catttggaaa tttgcaaaga tagtagtctt tctcaaagca tgttagttga
     3961 tccgaaaacc ttattatttg ggaactaaaa agaaaaagag cattttatta gaagtatgga
     4021 ttagggaata tagtagtagt gggctctaaa gattatgaaa tctttaagag ctctttttaa
     4081 gacgattgtt taaaacgaat taaccaagta ggagctttcc tagaatttat aaaattatag
     4141 tttcttgtat gacatgtata tagcatgcta aaataccaat aaataaggtt taagataaac
     4201 gcaaaaagtc gaaaaattaa cacttatttt gtgatattaa ttttaatttg caaaacaagc
     4261 tatatggaaa aagtttaaac tttttctaga gtccctatta ctagttaaaa agaccactgt
     4321 agtcctctga taaatcatcc taaagaccac agtagtcccc ttaaagtttt tccaaattaa
     4381 ctatctgaga aaggagagaa gatttttgaa tcaaggaata gctgatatta aattaatcaa
     4441 agaagtttta caagaatcta ccgcaaacgc ggtagcatct ggatcaggga ttagcttaag
     4501 tacagcaaaa aaactaaaaa gtggcgagag aaatgtagaa aaactaaatc tttctgatgc
     4561 aataaaaatc actgagtttg ctattaagaa taggcatgtg aaaattgaaa tttggaaatg
     4621 acggatacaa ctattatagt tttgtttttt ctaagtatca tatttatgaa tatacttttg
     4681 ctaaacattt aattatagat taatttttat ccaatatcta taattttcca attttttacc
     4741 ttttggagag taagggtaaa ttgaaagata ttgcttactt tagttgtatt attaagatac
     4801 ttaacaaaaa tttctccttg aatttctttt cctttcagtt taaaagtagg attaaccaat
     4861 tcaactaatt tcaaattact attaattggc tgaactgctt tgttttcaac atagtattca
     4921 agttcctttt ccgatgctga tggatacagt ttaaaaaagg tctctaaaaa atcggtgact
     4981 tcttccgtgg ttttagcatc aatgtcagat tcgattccta tttgttttga ttcatatttt
     5041 gccttttctg gctttgcaat catagtaggt aaactcgtta ctatatggct cccattttca
     5101 tcttcaaaga ttgtcactcg atatgcagaa gaaattgatt gtgtcttttt tccctctgta
     5161 atctgttgct cgactaaaaa tgaaacgata aattcttccg agtgtttttc aacatctaga
     5221 atttttatag attgaacttc ggaactcgtg ggaatatctg ctcgaaccat atcttggctt
     5281 aaagcgagac cgttttctgt aaggtactgt tcgagattac tcattctctt ttcaattgct
     5341 tccttgttat ttttccatga aaaatattct ttcgcaaaat cctctgtaaa actttcaacg
     5401 cctgaagtat tcactacctt ctcttttata actacttttt catgagtcgt atgctgatca
     5461 atggctgtga aatttctgta gattccaaaa gaaaggctac aagacaataa aagccaaaga
     5521 gcaagcacca tttttcgatg cttgcccact gaaatgactc gttctttatt ctttttttgg
     5581 ttcttttcct tttgattccg ttcaattttt atcttcatta tttttcctct tttctcttat
     5641 ttgtaccgtc cagcaccgat aagatgctgc tgccaatagc tttctgttag atttgtccag
     5701 cccactggat ctcctgcatg aaacatgcga ttctctcctg cataaatacc gacatgggtc
     5761 acataagtac cggcatcata agtcgaatga aagaaaacca aatcacctgg tttggcatct
     5821 tttacatcaa tatgggtcat ggcattatac tgatcttgag ccacccgagg aagtttcaga
     5881 ccaactttgg cataacacca ttgagtcaag ccactacaat caaacccagt ttcgggactt
     5941 gctccaccaa acacataagc cgttccttca tacttaaacg cctcatcaaa aatcccttgg
     6001 actgttttat catcaaactg tgttgtagtt aggtattgct tcactaataa gcaataaaac
     6061 atattgccat agttataccg ccacccacca tttttcttaa tcgctatggg attagggtaa
     6121 tcaacttttt ggccgctcga tttttccttt gaaaaagatt cggctaaagc aaaattatgc
     6181 ttcttcccgt ttttcgtgac atgatccaga tacccgccac catagttata cgcttgaata
     6241 atgctatctt gatcacaccc tagttctttg gaacgcttaa ccaaagacgc aaaatagaga
     6301 catccttgtt tgatggatgc ctcggtactt aatgaattcg gtggcaaccc ggctgactcc
     6361 gaactctgca tcacatcttc cattgtgcca ccagattcca cttgaataat cgccaaaaga
     6421 atcaggacat attctgagat gtttgattcc ttggcatatt tttccaccat tgctcggtgt
     6481 ttcaaaactt cttctgagac attacttccc ccagaagaag cacctgaact attacttcct
     6541 tcactgtctt ccccaaacaa caagcccaca cacatcaaga cgaccataaa ggagagtgtc
     6601 aagatggtta acgaaatgat acttaatttc tttacgttca ttttttctca cccgaccttg
     6661 attttggacg agggttcatt cttttttgta ctttttgatt ggttcgcttg ggcagagtag
     6721 acctctgttt cacttgttgg aaagaatttt tcttacttga tggttgactt attctttttg
     6781 aaacaatttc atgactagtt agttttcgtt tgatttcggg attcggattt actctcacaa
     6841 tctgttttgg tgcttctttt tttggcgttc tatctttttg aggttggcgc tctggtttta
     6901 tcgaaacatt ttcccttctt ttcttatgac gattaccggt ttcgttcaat actttgcgct
     6961 tttcagccat tttttcatct ctatgttgtc gttgtttttc tctcaactcc gctcgatttg
     7021 ctttttcttg gacaagtccg cgcttgaatt ctttcggtgc ttttttcgtc ttttcaattc
     7081 ctttgtggaa gttgtatttc aagttggtcg gcgtattcct tacctgatcc cttacttgtt
     7141 tagtcttatc tttcaccaaa gcttgggtat ccagaacttt gcccattttt ctcccagcca
     7201 aattcattcg ggattgtttc ttattatttg aaactggatt ctccttagtc gactcagatg
     7261 gtacctcgtg atcgttaggt aatcgtgaat tctttcgtag agaagaaccg gatggcttaa
     7321 atttggattt tgattgttcc tttgccaact tatgtccaac tgctgcacca ccgccaaaag
     7381 ccagagttcg gatcacattt cgattgagtt gccgcaattt acggttcatc aacatgtgcg
     7441 gtttccgcat gatgcgcctt cccattgact gactatcatt gctttggaga ttgaacatac
     7501 tcatgatgtc acctaatttg aaatagatac cggcaaaggt cacgatttgc aggaacgcaa
     7561 ccataaagaa aggataggta gccgaaatat tgaaaaacat ggtggagata ctaaaagcag
     7621 tcgtaatgac taaggtaact cccgctcgca tcataatcgt attgaataaa cgaataatcg
     7681 ccttctttcc caagctctca taagttggta acatggataa cagaaaacta attggcagaa
     7741 acatggcgaa aatgataaag agaatttggg agaacaacat aatccccgtc agtaagaaga
     7801 caaagaatga gataccaata ttaaacaaca ccaaaaagac cacggtccct agtcggttca
     7861 tggtctttgt aatacttaga ttcgcgttgt cattgtcttc aatttcagct tttaccgctt
     7921 cttctcgatc ctttccctta tttccatctg gactcacaga taaaatctta ttgacccggt
     7981 cttcaccaat ttcttctatg ttggaatcat caaattgcaa caagagccaa ggttgtcgga
     8041 cttgaatgga aaacaaacta tcacgaatca aatccacact gccttttcct ttgctttccg
     8101 aattcggcac aacgagctct gttccgagac taagagccgc ttcactaaca tccgaactaa
     8161 agttattgat ttttgtgata taggtaggcg catacgcgat aaaggaacca gagagaagga
     8221 agatcgttac aaagttaatg acagcccgga ctgctttggt agcttctcgc tttaatagtc
     8281 cgatatacgc cacatagact cctagagcta aaatcattaa taatagaaat ccaaaataga
     8341 aacccgagac ctgtaatcca ttttcagtaa taccggctag cgtttggata tttttcccaa
     8401 tactttctgc cgtatccgaa ataaaatcca acttgtacgc ttcttgtacc acatatccgg
     8461 tcgcatttga taaatacaaa ctaacggtcc aaatgaaatt tgtaatggcg tagagaccgt
     8521 acatcacact ctttcctaaa ccatctcccc agttccaagg cagccagccc cacgaactat
     8581 ctacaaagaa atccaattga tagttatcaa gcgaatattg agaatagaga ttgccagatt
     8641 taacggtatc atcaactaat ccagttgctt ccgctaatgt tccaatcaga ctcaaaagaa
     8701 gtaaaatggt gcagagacta attgcaatta ttcccacaat ccgtaaaagt ttctttttca
     8761 tctcctcact ccattctctt ttgaacaggc gggcgtgtat caaaagcatg gaagagttct
     8821 tcaaagactg gatgcacttg taccacacca acgcgaccat acaagtcctg catcaaacat
     8881 tgcccatttt ccaaatctcg cagacgcttt tgattgcttt catcttcttg atcaagacca
     8941 aagaattcga gtgtcttctt gatttcatga atatctgtgc ttcgaaaggc aaattttagc
     9001 ccaatgttgt ttttcagacg ttcattcaat aaatcatcac tattttgagt gacaaagtaa
     9061 acgccggcat tcatagcacg gccagcacga actagtttat tcgaaagagt ttttccttgt
     9121 gcgacttgaa gaaagctcca agcttcatcc aaatccacaa ttttaaagat ttctcgattg
     9181 ctatgaataa aatctagggc aaatgtagag ataacaatca acatagaaac actaagcaat
     9241 tccatcgtag tgtactcttc aaacgtagtc tccgcatctg gtaaaaccaa atctgccacc
     9301 tgaatgatat tcaattgttt atccaaacta atcgattgtg taattgaacc atccgaaaaa
     9361 agtaaatgcg caaaatcata atcggtaaac gattcaatat gatcggcaat attttccgca
     9421 actggcgagc cctcttttcg aagctcatta atgacttgta gcaatcctcg ttgctcactt
     9481 tgggtaacgg ttcgaatagc cttgcgtaaa acgggaaact tctccccatc tcgacttgaa
     9541 attcctgtta aaaaggtcag aatatcaatc gctagacttt ctgagtcttt ctttttcttc
     9601 ataatcacat aaggatcaag cagaccttgg ttttctggtt tactagttaa gttgagaata
     9661 ttgatttcat ccgtaatttc tggcaaggtt tctttccagc cacctcgttc tcctttggga
     9721 tcaacaatta ctgcttgacc gccaaataag acagcataat aaactaggag attattgcta
     9781 aaggatttcc caccaccaag tgaaccaaga aaagcagcag ccaatgcatt agtgatagaa
     9841 cctttgactc cttgtgaagc caatgccggt ttcagataaa cgttacgccc tgtatccaca
     9901 ttgtagccaa agtaaatgcc ttccaactct cctaacatct gtgtagctcc aaaaccaagc
     9961 ccagcaagaa aatcagacgt aacatactga atatagtcgt tcatatatcg tttacttgag
    10021 ggtaagaatt cttcatgtag ccccatcatg tcgccaaaag gacggactaa ttttacattg
    10081 gtatcatcat aaaaatcgaa cacttcatca caccggcgtt ttaattcatc caatgattct
    10141 gcactcactc gaacgacata gcttaatttg tacatggatt ctttggattg atccaacatt
    10201 gtttctagtt catccacgga gtccagagca tccaggacat tggaattggt ttcattgtcg
    10261 gactggtatg catgattgtc taagtctttc agttcttttt tcttattacg aactgtcgcc
    10321 agtgcctttt tattcgtcac gatttctaca ttcatactag tgtcgattgg gaaagtgaat
    10381 tgttgttgct ggtaataaaa gagttccgaa gaaggaaact ccatctcacc gacaatcgta
    10441 ttaattgtta gataggccac ataagattca tgattctcat gctccattcg taaatatcgt
    10501 ggcttctctt caatcaaaca gcgacttgga cgaagtaggt catagcgctt tatcaatgtt
    10561 tcagatttta gctttctctt cggtagttga aattcatatt cctcaaatgg tgttcccttt
    10621 tctccataaa tatgttcaat caaataagtt agatcactag gtgttactcg tcttactttg
    10681 aatctacgag ccaatttact ttccatcaat ttttcaagct tagtataccg gcgaatttct
    10741 tcattggata aagaaacaaa atcacccatc aaatgatgat tgaccccata aacaaactct
    10801 tgaagtccgg ataaaaaaga gttttttaga cttttcaagt tcacttcttc gtctgtgaca
    10861 atcagtttga agccaataaa aaagcgatag tcaatttgac tatcgccaat catcgatact
    10921 aaagcatctg tctgtaagtc gattctctgt tctgcgactt ctttcaaacg accagttatc
    10981 tcttttttcg accgttcctg cgtggcacga atgctgcttt ctgtcgcaat ttgtaaagca
    11041 tggatcctcc cttctctatt ctgagcaatc aattgacgga aattatcatg aacttgatac
    11101 ttttgctcag gagataagaa ggagtaatta tagggtacta actcatagta ggcaaaacac
    11161 tcgctatctt gattaaagac gaggttattt tcgatatact taatcgggta catcattcac
    11221 actcctcact aaggtaattg cttcattcca tcgtttcttc tgttctttga ctttctttcc
    11281 tgcaaaggta accttaaatc gcaagtgata actaataaag gatcgtaaaa accctaatgg
    11341 cttctttcca tcaaaggtct tttgcgacat aaaccaagcg actgccacag gtatgcccaa
    11401 gtattttaaa aatgccccct ctatcataga tagtggaggt acatgagcaa acacaatgac
    11461 aaacagcaag gataagacaa accaagccat ttgattaaag gttacaggaa aaggcagctg
    11521 aaagtcattg attgcgtaaa tgactttttc aactgcccag atactggtat agctttttat
    11581 ttttttcatt agtttcactt cctaaaaaag cgcctgataa agacgactct gcatgaatat
    11641 taattttctt tttaaaagtg tattccaact ttcaatctat caactgaggt ttatcagacc
    11701 ataatctgtg aatttcttgg tatccagctg aagtaataaa agtatatctt tgttgatata
    11761 ttctatcttg aaaatcacta aaccgtattt ttatttccca tttactcact gaatctttag
    11821 ggatactcat tacttcgtta ttatatttca tccaatataa ttcaaattct gttttttccc
    11881 tactcttaga atatctgtat caataggcgc tgaaactcct aacgcatcca gactatcatc
    11941 aatatgtttt tgagccgaaa gtaatgataa tagatttact tcatggtcgt ttgtgtcgat
    12001 agtttcttct aaatacacaa agtcaattga aaaagccatt cccagacctt cattaataag
    12061 ctcaaatggt aatttaaaaa catcattttc gtcagacttt accattttac cagcaaaacg
    12121 gtgttcagat attttgggta taagttttaa ataagggtaa taacttagac ggtcttgctc
    12181 ttcatttctt tgtttttcat cagcatattg ttgctttgat aaatcaagtg attcctcatg
    12241 ttgtctctct tgctgtttca tcgctttttt atgttgttta tttgtaaaaa aaagtgtgat
    12301 aaaacatact gcaaatgaag ttagtacagg gacaaatatg tctataaatg atttaaggtc
    12361 aaagtcaaaa ttcataataa tcataacctc cagcattata tatttatcaa taattatatc
    12421 cctaaaccta tatcatgaaa atgtttatcc tatgaattca aatgttcctc tatttgtcac
    12481 aagataattt ccttctagtt ccaagtcacg tccatatgct tcatagtcaa tatagttttg
    12541 caagtgtgta ggcacctctc ccaaagcacc tgtttcttca atcaaataat acgccacatc
    12601 actcatatcg ttacaatcgg gataacagat aatatcatcc acatgctcca ccatttcctc
    12661 aaaagagttg aagaaagcat gttggatttc caaagcaact tcaccaatcg gtgttccttc
    12721 tagttcctct gctaaaccac acaaccgatt gatttcctca attggtgtat attcatcaat
    12781 ctcaaaaggc aactcataat catggatcgc atactcttcg tattcgtcat ttaaaccaat
    12841 ccgttctttc acttcgtcaa aatcgacagg tgggctaaac caagccccca ccaattcacc
    12901 ttcgttatat tttccaaggt tcgcaatata tacacgcatc tgctccataa tggtccccct
    12961 tacgcaccga caatccgatt aaacaagttg agtaagacat ctttcacacc agaagcgttg
    13021 aagaccagtc ctaccgcaat cagcgcaatc accaaaaaac caatcagctt ggaaaactca
    13081 cgtttgaagc ctaaaaataa accaatgacc acaattgcca ataagactaa agattgggca
    13141 ttggttaaaa accatt
//
LOCUS       DACGLE010000076        12872 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0534
            SAMEA3304449-rid9560453.denovo.080, whole genome shotgun sequence.
ACCESSION   DACGLE010000076 DACGLE010000000
VERSION     DACGLE010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304449
            Sequence Read Archive: ERR985064
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12872)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12872)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702225.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702225.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 20:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,045
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 27 of 67
            Pseudo Genes (incomplete)         :: 25 of 67
            Pseudo Genes (internal stop)      :: 19 of 67
            Pseudo Genes (multiple problems)  :: 4 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12872
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304449-rid9560453.denovo.080"
                     /strain="VREN0534"
                     /db_xref="taxon:1352"
     gene            168..362
                     /locus_tag="IXT20_002273"
     CDS             168..362
                     /locus_tag="IXT20_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4860701.1"
                     /translation="MYYVEVKTKGVKNKQYVKGISNEYPLLGSWKEAAPFSKPCAIKI
                     KNELEKELTCGKAVVDIIEK"
     gene            631..930
                     /locus_tag="IXT20_002274"
     CDS             631..930
                     /locus_tag="IXT20_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4860702.1"
                     /translation="MDYTVEFDSELLKQFPQEEHFMYKEAISHKVTEYLEHNFFRIKP
                     VRTRSAKGIYEMKVKINKKNYRIAFALKKNHVFIFYISSNLQKIVFDKEVSKKIK"
     gene            complement(1027..1461)
                     /locus_tag="IXT20_002275"
     CDS             complement(1027..1461)
                     /locus_tag="IXT20_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ4860703.1"
                     /translation="MNIDSLMIDEISELYKSVGWTNYTKDTARLARAFEQSESLIKRN
                     GEGKIIGIVRWITDFATIAFIQDILIHPRYQRQGIGKVLLNEALEKITSYGPVQIELL
                     TDDTEKTKKFYESVGFVQVKEMGGISYVIDTRRKKVLFGNDK"
     gene            complement(1693..2403)
                     /locus_tag="IXT20_002276"
     CDS             complement(1693..2403)
                     /locus_tag="IXT20_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010780519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HAQ4860704.1"
                     /translation="MSCEEKIAAMKQRVLEANLALPRYGLVKLTWGNVSEVDRELGVI
                     VIKPSGVRYECMQADQMVVTDLSGNIIEEDSLKPSSDLPTHVVLYQTFEDITAITHTH
                     STHSVMWAQAGRDLPAYGTTHADAFYGKVPCTRQLTKEEVREAYEVHTGNVIVETFKE
                     RKLDPNEVPGVLVYGHGPFTWGDSPMKAVENSLILDEICLMAKENELINPNICEIPQY
                     LLDKHYFRKHGKYAYYGQ"
     gene            complement(2393..3253)
                     /locus_tag="IXT20_002277"
     CDS             complement(2393..3253)
                     /locus_tag="IXT20_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HAQ4860705.1"
                     /translation="MAQIGIYEKALPKDISWKERFSLVKEMDFDFIEMSIDETDERLA
                     RLDWSEEKIAELREEMFSSGVRIHSICLSAHRRFPFGSADPEKKKAAKQLMKKAIHLA
                     HNLSVKVIQIAGYDVYYEEKSMNSREDFIEGIKESVKEASKYGIILSVEIMDDPFMNS
                     ISKFLEIKEQIHSPFLQVYPDLGNLSAWPENNPARELEKGIDYITAIHLKDTYPVTKE
                     YSGQFRDVPFGSGCVDFLGLLRHLKRLDYDGTFLIEMWSEKSQDFRREIQQAKDYLSS
                     KLKEAGYDVL"
     gene            complement(3258..3896)
                     /locus_tag="IXT20_002278"
     CDS             complement(3258..3896)
                     /locus_tag="IXT20_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010740138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HAQ4860706.1"
                     /translation="MSRPNLQIALDHNDLEHALGDVMKVGDVVDIIEVGTILCLQEGQ
                     KAIRCIRSMFPDKKLVADTKCADAGGTVASNVAKAGADWMTVICCATIPTMEAAQKEI
                     GELQVELYGNWTFEQAMDWHNIGIRQVIYHQSRDALLSGETWGEKDLSKIKKLIELGF
                     NVSVTGGLNPHTLHLFEGIDVYTFITGRGITAANDPMKAAQNFKDEIIRIWG"
     gene            complement(3911..4210)
                     /locus_tag="IXT20_002279"
     CDS             complement(3911..4210)
                     /locus_tag="IXT20_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ4860707.1"
                     /translation="MRILVSCANGSGTSLMMMRSVEKAMKELNVPITKIHHCAISEGK
                     SSAGQYDVVFTPVNFLNMFTQAEKKGVTVIGIKNVMSVKEISEKFAETKWYQKNK"
     gene            complement(4253..5737)
                     /locus_tag="IXT20_002280"
     CDS             complement(4253..5737)
                     /locus_tag="IXT20_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HAQ4860708.1"
                     /translation="MGEFLLSIWTYFATNILTQPAYLIGFIVLLGYILLKRPLHECIA
                     GFLKATVGYFILTVGSGGLVNNFRPILVGLKERFNLDAMVTDPYFGQNAVDAGLMETF
                     GRTFGDVMILLLIAFVLNILLVRFQKYTKLRSVFTTGNVQVQQAATAFWILLFCFPDL
                     GRIQVLIFMGLILGCYWAVASNLTVGITQELTEGAGFAVAHQQMFGIFIFAKLAEWMK
                     KRDEKKNRSIKQDKKIEDIKLPGFLSIFNENMVATSLLMLFFFGIILIVLGKDYLIQA
                     QFMQEGQSFLFYIMTTSLNFAVYLAILQLGVRTFVDELTQSFQGISNTILPGAVPGID
                     VAATFGFGSPNAVTIGFLFGALGQFLMIGLLILLKSPTIVIAGFIPLFFDNAVIAVYA
                     NNRGGFKAACLFPFLSGIIQVGGSALFATWIGMSTYGGYIGMFDWATAWPLFTVIMKV
                     LGYAGIAVVVIGLLAIPQLQYRKNPDGYFLIVEDYDTYAEKQRV"
     gene            complement(5757..6218)
                     /locus_tag="IXT20_002281"
     CDS             complement(5757..6218)
                     /locus_tag="IXT20_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAQ4860709.1"
                     /translation="MLKYFFDHQLIHYPDKEITDWREAIAESCLVLIDKEIIDETYVE
                     EIIHCVEKYGPYIVIAPQVAMPHSSEKSDGVFGTAISFTKLKQPIRFESEGENEEKTA
                     SLFFTLAAKDPEEHLENIQKLSELLMTDGLVERLISTNSIDDYQVVMAEYN"
     gene            complement(6236..7303)
                     /gene="ulaG"
                     /locus_tag="IXT20_002282"
     CDS             complement(6236..7303)
                     /gene="ulaG"
                     /locus_tag="IXT20_002282"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HAQ4860710.1"
                     /translation="MSKTVNEVTKEKWLVETFPEWGTYLNEEIETEVVDPGTFAMWWL
                     GCTGIWLKSHEGTNILCDLWCGTGKRSHGNGMMKKGHQMMRMSGVQNMQPNLRTQPFV
                     IDPFAIKNVDALIVTHIHSDHLDIHTAAAVHQNCPEATFIGPKEVVRTWQKWGIPEEK
                     TITVKPGDAVKIKDINVVALEAFDRTALVTCEDPEEVLKGKMPQEMDQIAVNYLFETS
                     GGNLYHAGDSHYSNLFAKHGNEHKIDVCLGAYGENPRGITDKVTSVDMLRMAESLNAK
                     VVIPVHYDIWSNFMADPKEITEIWKFKKDRLKYGFKPYIWQVGGKFIYPTNKDDLEFN
                     FYRGFEDVFSIENDTPFPSFL"
     gene            7521..8291
                     /locus_tag="IXT20_002283"
     CDS             7521..8291
                     /locus_tag="IXT20_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAQ4860711.1"
                     /translation="MKNSLVNIEKRQKEILELLQQTEQLSTFELAESLDVSISTIRRD
                     LNLLEEKNEIIRNYGYCSYNYKNQTDFDQSGPERIKQAIAREASKYINDYDTLFINSS
                     STALKTLDFLQSNHLTVITNNVKINYSPHKDNCSYILTGGEMRFPKEALVGDIALNTI
                     SSVNADICIIGCSGVDVVHGVTTKILNESTINEMMVNKTIKCKILVADHRKIGLTSKF
                     KIADISVFDYLITDKFCSEKVLEEIRNTGIKTIQVDSF"
     gene            complement(8564..9391)
                     /locus_tag="IXT20_002284"
     CDS             complement(8564..9391)
                     /locus_tag="IXT20_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ4860712.1"
                     /translation="MIKVDGALLKEYRKKKGLTQVQLAEGICEQATVSNIENKNMSPG
                     INILSALCKKLNVEISDILVETEEDKIRKKINEIQSLNSVSKYQESYKILQTIDQEKV
                     SNSDLLNQILFYQGLLDHLYAKKPDAALFYFNQVLEQTTETDIHHLQAAANVAMIYLS
                     KGELDFAKVYVERTLKILSETDFDNLMVCIVYYDIATYYRKIEDYDKAIQLCEKGIEY
                     NKKHKSTYALEYLLYEIASCHKQLGEDDYLERYMDAKKIARFNGNDYAVKVIENDLK"
     gene            9959..11194
                     /locus_tag="IXT20_002285"
     CDS             9959..11194
                     /locus_tag="IXT20_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HAQ4860713.1"
                     /translation="MKTILFAPAVFNLAETTRMIEVAKAMKDRYYCVFFGFSKTFAPL
                     ITESGFEYHLLAPILTKKQEEQIIKFDQLRTLKNPFTKEMVASRITSEQSLIAKFEPR
                     LIVTGSNVTIFLSARNKKIPLVYVKPYALSEPFFQSEHALMPHELRFFGPLSNPLWHW
                     TKKRIVNTRWIPNAFKHAAQKEMLPLPKTSLQMMDADLNLITTPKLFLEEEQLPENYR
                     IVGPIFAKLSTSLPEEVLTFIQRKQQERKRIIYFAMGSSGNPRMLRKILFFLQKQPDL
                     AVIAPIDYLLDDIQSDSEDLLITEYLSSHLLHEFIDFSFIHGGEGTVQTACESGKPFI
                     GVGMQFEQSCNIDYCVKYGNAIALTKPVTERKLQIALREVSSPKIRQQARTLQAVFPI
                     NGPQQAIQEIETFLSNHPI"
     gene            complement(12193..12453)
                     /locus_tag="IXT20_002286"
     CDS             complement(12193..12453)
                     /locus_tag="IXT20_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HAQ4860714.1"
                     /translation="MYWLILFFVFIFLLTASHLILNMLATYHIQINRWIWALASFLIV
                     ILPKIIVPHMNVLFSWGTYVLCGIFAINFMIEQHRWFVTSKL"
     gene            complement(12480..>12872)
                     /locus_tag="IXT20_002287"
     CDS             complement(12480..>12872)
                     /locus_tag="IXT20_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ4860715.1"
                     /translation="FCKSKFLKEVGRFSIIPGIFNINEPIIFGAPMMLNPYMFIPFNL
                     VPLVLTIISYFAVKLEMVNRFTVLPTWTLPAPIGGYLSAGNDWRVVILIMINTLIAFI
                     IYYPFVKAYDKKMLSDEQSRGENEVQTV"
BASE COUNT         4107 a         2606 c         2021 g         4138 t
ORIGIN      
        1 taagaacaat agccttttat tatatatatt attcaaatta gttatatcga gtgaaaaaaa
       61 ctttcaaatt gattcttttt atttatcata aagatataga tacataatca ttttcactta
      121 catatatttg aatattaact aaaaacagca agaaagaaga ggatcaaatg tattatgtag
      181 aagtaaaaac aaaaggcgtc aaaaacaaac aatatgtgaa aggtatcagt aacgaatatc
      241 ctttacttgg ttcatggaaa gaagctgctc ctttttccaa accatgtgcc ataaaaataa
      301 agaacgagct agaaaaagaa ctgacttgtg gtaaagctgt tgtagatatc atcgaaaagt
      361 aaatccaatg tatagaaagg aagatcacac atgaaaagcg acgtacaaat tgctaagatt
      421 atagaattag aagaagaaga cgcagtgttg acaattgaaa aaaaataaat gtttgtatac
      481 catatgatta tttcaatatg tatcctggaa ttggagatct cgtagagcta tatcaagatg
      541 aatcaaatat acttgtcatt catcatgaaa tatagagact tataaaattg tttattttta
      601 taatgtatca gtatagaaag gaaataaaac atggattata ctgttgaatt tgacagtgaa
      661 ctacttaagc aatttccaca agaagaacat ttcatgtata aagaagcaat ttcccacaaa
      721 gtgacagagt atttggagca taattttttt cgtataaagc cagtgcgtac tcgttcggca
      781 aaaggaatat acgagatgaa agtcaaaatt aataaaaaaa attatcgcat agcatttgct
      841 ttaaaaaaaa atcatgtgtt cattttttat attagttcga atttgcaaaa aattgtattt
      901 gataaagagg tgagtaaaaa aataaaatag ctgagcattg gaggatagat tgagtgatac
      961 taagtctatc ctcttgtttt tctaaagaac taagctagaa ttttttcttt tgtacgttat
     1021 tatatgttat ttgtcattac caaaaagcac cttttttctt ctagtatcta tgacataaga
     1081 aataccaccc atttccttta cttgaacaaa tcctactgat tcataaaatt tttttgtttt
     1141 ctcagtatca tctgtcaata attcgatttg gactggtcca taactagtga tcttctccaa
     1201 agcttcattc aaaagcactt tgcctattcc ttggcgctga tacctcggat gaatgaggat
     1261 atcttgaata aatgcgatcg tcgcaaaatc agtgatccaa cgaacgattc cgataatctt
     1321 cccttcccca ttacgcttta tcagagattc agattgttca aatgctctag ctaaccttgc
     1381 agtgtctttt gtgtaattcg tccaaccaac gctcttatat aattcagaaa tctcatcgat
     1441 cataagggaa tcaatattca tttttttctt cctttcttat acaattctcc ctctaataat
     1501 acagttcttt gactatttaa tctatcacat ataactctcc tgtaaagatt ctctcgattc
     1561 cttaaaattc ctctttcctt ttagaatgca tactttttga taaataaaaa ggttgtgata
     1621 aattttcttc acaacctctg tttctctcta tcaagtgtta tcttttaatt ttctaccctt
     1681 accctagcat ctctattgcc cgtagtatgc atactttcca tgtttgcgaa aataatgttt
     1741 atctagtaaa tattgcggga tttcacatat attggggttg atcagctcgt tttctttggc
     1801 catcagacaa atttcatcta agatcaaact gttttctact gctttcatag gtgaatcccc
     1861 ccatgtgaac ggtccatgtc catagacaag tactccaggg acttcgtttg gatctaattt
     1921 tctttcctta aacgtctcga caatcacatt ccctgtatga acttcgtaag cttctcttac
     1981 ttcttctttc gttaattggc gtgtacaagg aaccttacca taaaaagcat cagcatgagt
     2041 cgtgccataa gctggcaaat cgcgtcctgc ttgcgcccac atgacggagt gcgtggaatg
     2101 agtatgagta atagcagtta tatcttcaaa agtttgatac aaaacaacat gtgtggggag
     2161 atctgaagat ggttttaagc tatcttcttc tattatattt cccgacaaat ctgttaccac
     2221 catttgatct gcttgcatgc attcatatct gacaccgctt ggcttaatga cgatgactcc
     2281 tagctcacgg tccacttcac tcacattgcc ccaagtcaat ttgactaatc cgtatcgcgg
     2341 caatgccaga tttgcttcaa ggacacgttg cttcattgct gcaatttttt cttcacaaga
     2401 catcatagcc agcctccttc aatttagaac ttaagtagtc ttttgcctgc tggatttctc
     2461 ttcgaaagtc ttggcttttt tcactccaca tttcaatcag aaatgttcca tcataatcta
     2521 gacgtttcaa atgtcttaat aatccaagaa aatcaacaca accgctacca aaaggaacat
     2581 cacgaaactg gcctgaatat tcttttgtta cagggtaagt atcttttaag tggatagcag
     2641 tgatataatc gatccctttc tcaagttctc gagccggatt attttccggc catgcggaca
     2701 aattgcctag gtctggatat acttgtagaa aaggtgaatg aatctgttct ttgatttcta
     2761 aaaattttga aatggagttc ataaatggat catccatgat ttccacggaa agaatgatgc
     2821 catatttaga agcttccttt acgctttctt ttattccttc gataaagtct tctctggaat
     2881 tcattgattt ctcttcataa taaacatcgt atccagctat ttggattact ttgacgctta
     2941 gattatgcgc caaatggatt gcttttttca ttagttgttt cgctgctttt ttcttttctg
     3001 gatcagcaga accaaacgga aatcgccgat gcgcacttaa acaaatcgaa tggatacgta
     3061 caccggagct gaacatctct tctctcagct cagctatctt ttcttcagac caatctaatc
     3121 gtgctaaccg ctcatccgtc tcatcaatcg acatctcgat aaaatcgaag tccatttctt
     3181 ttacaaggga aaaacgttct ttccatgaaa tatctttggg taacgctttt tcataaatcc
     3241 caatttgagc catttcctta cccccagata cgaatgatct catctttgaa gttttgtgcg
     3301 gccttcatcg gatcatttgc tgctgtaata ccacgaccag tgataaaggt atacacatcg
     3361 atcccttcaa ataagtgtaa tgtgtgtgga tttaatccac cagttacaga aacattaaag
     3421 cctagttcaa tcagtttttt gatcttcgat aaatcttttt caccccaagt ttcaccagat
     3481 aacagcgcat cacggctttg atggtagatt acctgccgga taccgatgtt atgccaatcc
     3541 atggcttgtt caaaagtcca attgccgtag agttctactt gcaattcacc tatttctttt
     3601 tgtgctgcct ccatagttgg aatcgttgca caacaaatga ctgtcatcca gtctgctcct
     3661 gcttttgcaa cattactcgc cactgttcct ccagcgtctg cacatttcgt atcagcgact
     3721 agttttttat ctgggaacat actgcgaata cagcgaatcg ctttttgtcc ttcctgtaag
     3781 caaagaattg tgcccacttc aataatatct actacatcac caactttcat aacatctcct
     3841 aatgcatgtt ctaagtcatt gtgatctaac gcaatttgca aattcggcct gctcatctta
     3901 gttccttcct ttacttgttt ttttggtacc actttgtttc agcaaatttt tcactgattt
     3961 ctttgactga catcacattt ttgataccga taactgtaac tccttttttc tctgcctgag
     4021 taaacatatt caaaaaattg actggcgtaa aaactacatc gtattggcca gctgaacttt
     4081 ttccctctga aatggcacaa tgatggattt tggtaattgg tacgtttagt tctttcattg
     4141 ctttttccac acttctcatc atcatcaagc ttgttcctga tccatttgca cacgatacta
     4201 aaattctcat cttaatccct ccattatgag tgttgtctaa tcagattttc ttttatactc
     4261 tctgcttttc tgcatatgtg tcatagtctt ctacaatcaa aaaataacca tcaggatttt
     4321 tccgatattg taactgcgga atagccaata aaccaatgac aactacggca attccagcat
     4381 aacctaggac tttcatgatt actgtaaaca atggccaagc tgttgcccag tcgaacatcc
     4441 cgatataacc accgtatgtt gacatcccaa tccatgtagc gaataatgct gaaccgccta
     4501 cttgtatgat tcctgaaaga aacgggaaca ggcaagcggc tttgaatcca cctcggttat
     4561 tggcataaac tgctataacg gcattatcaa agaacaacgg aatgaaaccg gctatgacga
     4621 tcgttggtga tttcaataaa atcagtaaac caatcatcaa aaactgtccc aaagcaccaa
     4681 acaagaaccc aatcgttacc gcatttggcg aaccaaagcc aaaagtagca gctacgtcaa
     4741 ttcctggaac agctcctggt aaaatcgtgt ttgagattcc ttggaaagat tgagttagtt
     4801 catcgacgaa tgtccgaaca cctaattgca agatcgccag atataccgca aaatttaaag
     4861 aagtcgtcat aatgtagaat aagaaactct gcccttcttg catgaattgc gcttgaatca
     4921 aataatcttt gcctagtaca atcagaatga tcccaaagaa aaatagcatg agtaacgaag
     4981 tagcaaccat attctcattg aaaatcgaca agaatcctgg taatttgata tcttcaattt
     5041 ttttgtcttg cttgattgat ctatttttct tttcatcccg ctttttcatc cactcagcta
     5101 attttgcaaa tataaagatt ccgaacattt gctgatgtgc cactgcaaat ccggcacctt
     5161 ctgtcaattc ctgcgtgatt cctactgtta agttagaagc tactgcccaa taacaaccta
     5221 aaatcagtcc cataaaaatc aatacttgta tcctacctag atcaggaaag caaaacagta
     5281 aaatccaaaa agcggtagct gcttgttgaa cctgaacgtt tccggtagtg aaaacggaac
     5341 gcagtttcgt atatttctga aaacggacta ataagatatt taaaacaaat gcaatcagta
     5401 ataaaatcat cacatctcca aaggtacggc caaatgtctc cattaatcca gcatccacag
     5461 cattttgtcc aaaataagga tctgtaacca tggcatccaa attgaaccgt tcctttagtc
     5521 caaccaagat tggccgaaaa ttattgacca aaccaccaga gccaacagtc aaaataaagt
     5581 aaccgaccgt tgcctttaag aatccagcga tgcattcatg aagcgggcgt ttcagcaaaa
     5641 tatagcctaa taacacgata aaaccaatca ggtatgctgg ctgcgtcaag atatttgttg
     5701 caaaatacgt ccaaatgctt aacaaaaatt cccccatctt ttttctcctc tatcaattaa
     5761 ttatattctg ccatcacaac ttggtaatca tcgatggagt tcgtagagat gagacgctca
     5821 accaatccat ctgtcattaa taattcggat aatttttgga tattttctag atgttcttct
     5881 ggatcttttg ctgcgagtgt aaaaaacaaa ctagccgttt tctcctcatt ctccccttca
     5941 ctttcaaaac ggattggctg tttcaacttg gtaaatgaaa tagctgttcc aaaaacacca
     6001 tcactttttt ctgaagaatg cggcattgct acttgaggtg cgatcacaat ataaggaccg
     6061 tatttttcta cacaatggat gatctcctcc acatacgttt catcaataat ttccttatcg
     6121 atcaatacaa gacagctttc ggcgatagct tcacgccaat ccgtgatttc tttatctggg
     6181 taatgaatca attgatggtc aaaaaagtat ttcagcaaga aactcccctt ctttcttata
     6241 aaaacgacgg aaacggtgta tcattttcaa tcgagaagac atcttcaaat cctcgataaa
     6301 aattaaactc aagatcatct ttgtttgtcg gatagatgaa tttcccgcct acttgccaga
     6361 tataaggttt aaatccatac ttcaggcgat cctttttgaa tttccaaatt tcagtgatct
     6421 cttttggatc agccataaaa ttagaccaaa tatcgtagtg gacaggaatc actacttttg
     6481 cattcaatga ctccgccatg cgcagcatat caacagaagt aactttgtct gttatccctc
     6541 gtgggttttc accataggcc cctaaacaga catcgatttt gtgttcatta ccatgtttgg
     6601 caaataaatt ggagtaatgt gaatctcctg catgataaag attccctcct gatgtttcga
     6661 ataaatagtt tactgcaatt tgatccatct cctgaggcat ttttcctttt aatacctctt
     6721 ctggatcttc acaagtgacc aacgctgtcc gatcaaacgc ttctaaagct actacattga
     6781 tgtctttgat tttgactgca tcacctggct ttactgtaat cgttttttct tcaggaattc
     6841 cccatttttg ccacgtgcga accacttctt ttggcccaat gaatgttgct tcaggacaat
     6901 tttgatgaac tgctgcggct gtatggatat ctagatgatc ggaatggata tgtgtaacga
     6961 ttaaagcgtc tacatttttt atcgcaaaag gatcgattac gaaaggctga gtacgtaaat
     7021 taggctgcat attttgaacg ccactcatac gcatcatctg atggcctttt ttcatcatgc
     7081 cattcccatg actacgtttt cccgtcccac accatagatc acataaaata ttcgttcctt
     7141 catgactttt cagccaaatt cccgtacagc ccagccacca catagcaaaa gttccaggat
     7201 ccactacttc tgtttcgatt tcttcattca agtatgtgcc ccactcagga aatgtttcta
     7261 ctaaccactt ttcttttgtt acttcattga cagttttact catttgcttg ctccccccat
     7321 atcgttgttc caacttacaa gtaaatatta atgtgtatca gcatccatga aaaccctttt
     7381 atatgaccgt ttgtttttta tatgattttc tttcagtaat caatgattgt ttcagtataa
     7441 taaagacaga tatgaatgtt taaatgaaca actagtcaaa taaatccatg gatcaagatg
     7501 tccctcagga ggcaaaagtc atgaaaaact cacttgtcaa tattgaaaaa cgtcaaaaag
     7561 aaattttgga actgcttcag cagactgaac aactatcaac tttcgaatta gctgaatccc
     7621 ttgatgtatc tattagtacg atacgaagag atttaaattt attagaagaa aaaaacgaga
     7681 tcatacgcaa ttacggttat tgttcttata attacaaaaa ccagactgat tttgatcaat
     7741 ctggtcctga aaggattaaa caagcgattg ccagagaagc tagtaaatac attaacgatt
     7801 atgatacgct gtttatcaat tctagctcga ctgcgttgaa aacactggat tttcttcaat
     7861 ctaaccattt gacagtcatt acaaacaacg tgaaaatcaa ttactctccg cacaaagata
     7921 attgtagtta tattttaact ggtggagaga tgcgctttcc aaaagaagca ctagtgggcg
     7981 acatcgcatt aaatacgatt tcttcagtca atgcagacat ttgtatcatt ggctgtagcg
     8041 gagtcgatgt agtacatggg gtcacaacaa agatattaaa tgaatcaacg atcaacgaaa
     8101 tgatggtcaa caagacaatc aaatgtaaga ttttagtcgc tgatcatcga aaaatcggac
     8161 tgacatcaaa gttcaagatt gctgatattt cagtctttga ttatttgatc actgataaat
     8221 tttgttctga aaaagtatta gaggagatca gaaatacggg gataaaaacc attcaagtag
     8281 attcatttta agaaccagga cgtaaatatg taaataggat tatcaaaaaa atgaataaca
     8341 aatttatcga taaatgaaaa acacgctacg ctcaaattga taaaggaatc ccaagatgta
     8401 tccatccaat cgataccatc ataatctatt gcttctttta taaccgttat atgttgccta
     8461 aataggaatc aaacaatatc gaaaaaagga ggtatgacaa aagtcacacc tccttttttc
     8521 gaataaacgg tattcaaatc agaacactga tttcacaacc actttatttc aggtcgtttt
     8581 caatcacctt taccgcgtaa tcatttccgt tgaaacgcgc tatttttttt gcgtccatat
     8641 atctttctaa ataatcatct tctcctaatt gcttatggca agaagcaatt tcataaagca
     8701 agtattccaa tgcataagtc gatttatgtt tcttattata ctcaataccc ttctcacata
     8761 attggattgc tttatcataa tcctcgattt tacggtaata ggtggcgata tcataatata
     8821 caatgcagac catcagatta tcaaaatcag tctcactcaa tattttaagt gtacgctcca
     8881 cataaacttt cgcaaaatct aattctcctt ttgacaagta gatcattgcc acgtttgcag
     8941 ctgcttgtaa gtgatggata tctgtttcgg tcgtttgttc caacacttga ttaaaataaa
     9001 ataatgctgc atctggtttt ttcgcataaa gatgatcaag cagtccttga taaaataaaa
     9061 tttgatttaa aagatcagaa ttacttactt tttcctgatc gatcgtttgt aaaattttat
     9121 aagattcttg gtattttgat acagaattca atgattgaat ctcatttatt tttttcctga
     9181 ttttgtcttc ttctgtttct actaatatat cggagatctc gacattcagc tttttgcaaa
     9241 gcgcacttaa gatattgatg cctggcgaca tattcttgtt ttcgatattg ctgacagttg
     9301 cttgttcaca aatgccttct gctaattgta cttgtgttaa cccctttttc tttcggtatt
     9361 cttttagtaa tgctccatct actttaatca aaacaatttc cttcccttta aaaaattatt
     9421 tctcctttat atcatacaca aaaaaaagtt ctaaacaacc aaaaatgttt agaacagaat
     9481 aaaaatttta attttaaaat aatattcaaa ttaaacaact ggcgtatctg ctccttgatt
     9541 tctttttgtg ttatctgaat aattatcgtt tacagttata acacatagtg aaaattgtca
     9601 tggcgagaaa gtctaagaat atttttaaac ataactattt ctttcttacg tgtgtataat
     9661 gacgacagca cacctatcaa ttaatataat tattttattt ttttatgttc tcatgtttat
     9721 ttttaatgac acaacttctc gtatttatct ggcacaacat ataaatcgat caaaaaattg
     9781 ttgcgtgttt ttttgaaaat tggagtttca acttgattac aaaatttaaa tttagaagtt
     9841 gtgaaaacag cgagagacag tttaatattc aaaatataca taggctactc gaacgctgtg
     9901 ttttttagta tactagtaag aaacgaaggg atagactcat tccaataagg aggatttcat
     9961 gaagacaatt ctttttgctc ctgcagtatt caatttagct gagacaacac gaatgattga
    10021 ggttgccaaa gctatgaaag accgttatta ctgtgtattc tttggctttt ctaaaacttt
    10081 tgctcctttg atcacagaaa gtggctttga gtatcattta ctagcgccga tccttacgaa
    10141 aaaacaagaa gaacaaatca taaaatttga ccagttgcgt acacttaaaa acccttttac
    10201 caaagaaatg gttgcctctc gtatcactag tgaacagagt ctgattgcta agtttgagcc
    10261 tcgactgatt gtgacaggtt cgaatgtgac aattttttta tcggcccgca ataaaaagat
    10321 tcctttagta tatgttaaac cctatgcttt gagtgaacct ttttttcaat cagagcatgc
    10381 gctgatgccc cacgagttac gtttcttcgg ccctttaagc aatccccttt ggcactggac
    10441 aaaaaagcgt atagtaaata cccgatggat ccccaatgcc ttcaaacacg ctgcacaaaa
    10501 agaaatgctt cctttaccaa aaacaagttt acaaatgatg gatgctgatc taaatctcat
    10561 cacaacgcct aaactatttt tggaagaaga acagctgccg gaaaattatc gaatcgttgg
    10621 tccaattttt gctaagctca gtacttcctt gcctgaagaa gtgctaactt ttatacagcg
    10681 aaagcaacaa gaaagaaaac gtattatcta tttcgcaatg ggtagttcag gcaatccgcg
    10741 tatgttacgc aaaattttgt tcttcttaca aaaacaaccg gatctagcgg ttattgcacc
    10801 tattgactat ctgcttgatg acattcaatc tgactcagaa gatcttttaa taacagaata
    10861 tctgtctagt catcttttgc atgaattcat tgatttttct tttatccacg gcggggaagg
    10921 gaccgtccaa actgcctgtg aatccggcaa accgtttatc ggtgtgggga tgcaatttga
    10981 gcaaagctgt aatattgact attgtgtaaa atacggaaac gcaatcgccc tcacaaaacc
    11041 agtgactgaa agaaaactgc agattgctct gcgcgaagtt tcttcaccca agattcgtca
    11101 gcaggcacgc acgctgcaag ccgtttttcc aataaatggt ccacaacaag caattcaaga
    11161 aatcgaaacc ttcctgagca accacccaat atagtttcaa gtagaaaaag cttttttaat
    11221 agagactcat cagtaatttc gctattcgag acccagacag tgaaaataca aaaaaagtaa
    11281 atagtggatt gaaaagttag agttacttaa attacggtgt ctaaaaaaca caaaatcatc
    11341 cttaaaataa aagatctgac gctatccaaa tgagcatagg tagcgtctgc catttattct
    11401 tagtcaaatt ctttgacacc tttctctggt tggatcatct caacgtttct tcaaaagaaa
    11461 gaccataata attaggcaaa tacattttct taaaatgtgt catatagatc tagaagcatc
    11521 gctttgtaga gatttgtgcc gctagtcccc tcaacaatta ctaaatcaaa tgtaggattt
    11581 accactttat ctttgataat caacctccct cttctgttga gacaatgatc tcgcaatccg
    11641 atgcatactc ctatataaac tgccagcaat ccccacatat tgagtagatc atgtcagaat
    11701 gttctatgtc gtcagcaacg atacactaaa agtcacgttc accatgactg attgctattc
    11761 ccagcacaat tatttctata cacgtttcat gatttgaata aacactcaca tcatacatat
    11821 taccattcat acaccatact gcagccccta ctatatggtt gtaagctccc aaatctatat
    11881 ttgacttgat cactttggta gtagatctac taattctaaa tccattgctg ataaaatttc
    11941 cattttttcc tccttctttc tgccaaaaca gttaaaacaa taataatctt tatgtaaaaa
    12001 agggctgtga caaaagcctt gtcacagtcc catattccga ataaacggtg gtcaaaagct
    12061 tgcatctgcg gtaataagtt caaccaaacg aaaaataagc caacatttca caaaaattta
    12121 agaagcaatt ttcgtaaatt tcttcttatt tcttgaagct aaccacttct gttacaacca
    12181 cttttagaac atttaaagct tcgatgtgac aaaccatcta tgctgctcga tcataaaatt
    12241 gatagcaaaa attccacaaa gtacatatgt cccccatgaa aacaaaacat tcatatgcgg
    12301 tacaatgatc tttggaagaa tcacgatcaa aaatgatgcc agtgcccaaa tccatcgatt
    12361 gatttgaata tgatatgtag ctagcatatt caatataagg tgactagccg ttaataaaaa
    12421 gataaaaaca aaaaataaaa tcagccaata cattgtttcc caccttctaa aaaccaaact
    12481 tagacagtct ggacttcgtt ttctccacga ctttgttcat ctgacagcat ctttttatca
    12541 taagctttta caaatggata atagataata aatgctatca atgtgttgat catgatcaag
    12601 ataacaacac gccagtcatt tcctgctgaa agataaccgc cgattggagc tggtaatgtc
    12661 catgttggca aaacagtaaa tctattgacc atttctaatt tgactgcaaa ataactgata
    12721 atagtcaaaa ctaaaggaac taagttgaac gggatgaaca tgtaaggatt caacatcata
    12781 ggagctccaa aaattatggg ctcattgata ttgaagatac caggaataat agaaaaacga
    12841 ccaacctctt tcaagaattt tgatttacag aa
//
LOCUS       DACGLE010000077        12670 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0534
            SAMEA3304449-rid9560453.denovo.081, whole genome shotgun sequence.
ACCESSION   DACGLE010000077 DACGLE010000000
VERSION     DACGLE010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304449
            Sequence Read Archive: ERR985064
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12670)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12670)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702225.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702225.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 20:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,045
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 27 of 67
            Pseudo Genes (incomplete)         :: 25 of 67
            Pseudo Genes (internal stop)      :: 19 of 67
            Pseudo Genes (multiple problems)  :: 4 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12670
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304449-rid9560453.denovo.081"
                     /strain="VREN0534"
                     /db_xref="taxon:1352"
     gene            6..305
                     /locus_tag="IXT20_002288"
     CDS             6..305
                     /locus_tag="IXT20_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ4860716.1"
                     /translation="MKNRLIVACGSGVATSQTIASKIQSMLEDDGIAFPVEAVDYKSI
                     QNELLTAGIYVYVAQPDEEVLEQAKDLGIEVFPGIPFLTGMGVEPIYDSIKELVQ"
     gene            378..1739
                     /locus_tag="IXT20_002289"
     CDS             378..1739
                     /locus_tag="IXT20_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HAQ4860717.1"
                     /translation="MEVFQTIINYVLNLGSAIFVPLIILILGLLAGMKLKKAFMSALT
                     LGIAFSGMSMVIGYMSNAVSPASEALAKNTGISLPALDLGWTGAASITWSWSYAFIFF
                     AVTIGVNFVLLLFNWTKTLNVDMWNVWGKALTAYLVYYVSGSLAAGFLTAMVQVILEL
                     KLGDMFQKHIQDLTGIPLVTVTHFMTSAAVLLLPFNMIMDKIPALNKRADTNALKKKI
                     GIFSENSVMGFIIGLGLGFAAAYGVSGSLNLAIQTATALSLFPMISKMFMQSLSPLAD
                     AMSEFMKKRFKDREVYIGLDWPILAGRSEIWVTAILLVPVFIGYAIILPGNQVLPLAG
                     IINYSIAVGGLLLTGGNLARMLILGIITMPIYLYGATYLAPILSDLAEKSGAVEGIKK
                     GQLITWSSIEGPEFRVLFAEAFNRNILAIVGCVIFLGLFVLLYKYMIKAKVPSERYAE
                     LQK"
     gene            1761..2021
                     /locus_tag="IXT20_002290"
     CDS             1761..2021
                     /locus_tag="IXT20_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4860718.1"
                     /translation="MKREETDKIKWTVALCGTLLLFLYGLFTQNIIINLLVIFFALVI
                     YKYGNHVLFREYDEKRKRKIEESMKIKEATKEILREKSFIKR"
     gene            2035..2787
                     /locus_tag="IXT20_002291"
     CDS             2035..2787
                     /locus_tag="IXT20_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HAQ4860719.1"
                     /translation="MSDGRMLFERERQDMARIVRVIFERKNTNVAGGNFSFKTTDDQG
                     KDYIIMTPTMMSEAYLGEISPAQVLVVEPRTRRIVDGVGHLTREINMHEAMYETNPAI
                     KAVLHAHAPNAMFWATTGLPMPNLTEATAKVKEIPVMDFEPNCSEELAELTSDYVKKQ
                     HLELPHMLLLNSHGVLINTGGESGIEAIHKALQILDTVEWNAEIAYKQTIFQALGITD
                     GFYSKGEKIGGLEELKHKTAIYNKSVIAAGGD"
     gene            complement(2881..3486)
                     /locus_tag="IXT20_002292"
     CDS             complement(2881..3486)
                     /locus_tag="IXT20_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAQ4860720.1"
                     /translation="MTYIFEKGTSGKHLILLHGTGGDEHSLLDIAHFLAPNSTLLSFR
                     GTVQEDGMNRFFKRNGLNQFDLDSLEKETDKLHEKIKEISEKELIPLSDWLLVGYSNG
                     ANIAAHLLLERETELNKGLFFHPMSLGVHQSRSTLEDKTVWISYGDNDPIVSPASFKE
                     LADQFETRQANVTIQLTVTGHQLTMDEIKQAREWTDHLLTK"
     gene            complement(3539..4513)
                     /locus_tag="IXT20_002293"
     CDS             complement(3539..4513)
                     /locus_tag="IXT20_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
                     /protein_id="HAQ4860721.1"
                     /translation="MDTQIRGIHHVTAMTSSATKIYRFFTDILGMRLIKKTVNQDDIE
                     TYHLYFTDDTGAPGTDMTFFDFPNQPKGKKGTDTISRASFRVPSDAALDFWLERFEKY
                     DVTHSAINERFGKKYIEFEDFDNQYYQLISDENNQGVAAGIPWKNSNVPTEFAITGLG
                     PVFVRVSQLEHIRLILEKILGFKETDHSGDFYLFEIGEGGNGASIIIEHRTDLPKAIE
                     GFGNIHHLALRVADKEALEYWIQVINKIELPNSGFVDRFYFQSEYFLAAHHVLFELAT
                     DGPGFLEDEPAESAGEKLSLPPFLEERREAIEAYVRPFDTSDANKKRE"
     gene            complement(4891..5670)
                     /locus_tag="IXT20_002294"
     CDS             complement(4891..5670)
                     /locus_tag="IXT20_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ4860722.1"
                     /translation="MLLRVVGSIIFRYTLEEVNNVPKYEEIANILRERIRSKEYPLDS
                     FLPNQVDLVKEFGASRMTIKKAINILAMEGLVYSQRGSGTKILNHPFMQKDTSPANEY
                     EGLSLQMKRRHRSLKSQIIHFDVEFPNQYIQERLMIDAEQPVYNIHRLRILDGEPYVL
                     EHTFMPVHLVPGLKKEHLLASIYDHLHQELNVHFAGAYRAISADKSDEYDQQYLNCAE
                     NDPVLEVQQVVYLKDGQPIEYSSSRNRFDVRNYSVLDVRST"
     gene            complement(5737..7008)
                     /gene="celB"
                     /locus_tag="IXT20_002295"
     CDS             complement(5737..7008)
                     /gene="celB"
                     /locus_tag="IXT20_002295"
                     /EC_number="2.7.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HAQ4860723.1"
                     /translation="MNGFMDKLSEKIMPLANLLGQNRYLTVLRDAFMLSFPLTMFGSI
                     VVVINNLPFFSDATKGTLSNLFGNGQNATMSIMSVFVTFGIGYYLSKSYDVEGIFGGA
                     VSFASFLILTPFAMKTAGGEDVTGVLSLDRLGAKGMFIGMIAAFLAGEIYCRITKRGW
                     QIKMPDGVPPAVTKSFAALIPAVVTLTIFLVINAVMTGIFNANLHDVVYEVIQKPLTG
                     LGSSLPATLIALFFVQFLWFFGLHGQIIVNSVMDPIWNTLMLDNLEAYQHGKELPHII
                     TKPFMETFTVGIGGSGMTLAVVLLMAFVLKKKQYRDVGRLALAPGIFNVNEPAIFGLP
                     IVLNATILIPWVVAPLIVTTFNYLVMAAGLVPAPTGVSVPWTVPIIASGVLATNSWLG
                     GMLQVIDFVIVAFIWYPFLKVLDKQPDLDIA"
     gene            7161..8558
                     /locus_tag="IXT20_002296"
     CDS             7161..8558
                     /locus_tag="IXT20_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAQ4860724.1"
                     /translation="MKTYTFPENFWWGSAASGPQTEGRVSDDGKGENIWDHWYELEPE
                     KFFDQVGPEKTSQVYTKYKEDVQLMKETGHNTFRTSIQWSHLIPEGTGEINQKAVSFY
                     RDFFTELLDNGIEPFVNLYHFDMPLPLQEKGGWLSRETVDAYVSYAAACFELFGDLVK
                     KWFTHNEPIVPVEGGYLYQFHYPNHINMKEAVQVGYHENLASAKVIKAYHEGGHDGEI
                     GIILNLTPSYPRDETNPEDVKAAKIADAFFNRSFLDPAVKGEFPQELIEIVKELAIMP
                     EIHEGDLELIRENTIDLLGINYYQPRRIKAKETPINHEHSPMPDDYFDNYDMPNKKMN
                     PYRGWEIYEKGIYDILTNTRENYGNIKCYISENGMGVEGEERFVNSEGIIEDDYRIEF
                     VTDHLKYVHQAIQEGTNCVGYHMWTCMDNWSWTNAYKNRYGFIAVDLAQDGKRTIKKS
                     GRWFKTVSEKNGFDA"
     gene            8639..9079
                     /locus_tag="IXT20_002297"
     CDS             8639..9079
                     /locus_tag="IXT20_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HAQ4860725.1"
                     /translation="MVDTYRNILVAIDGSEKAEKAFLEAITIAKRNQATLHILYVNEV
                     TGNYFGDFAFVTTSLQEELDEVAENQMKEHRNLAIEKGLTDIETYVLYGYPKTLIANF
                     HESKEPIDLIVMGATGLNAVERALVGSTTSYVVNHASCNVMVVK"
     gene            9227..9553
                     /locus_tag="IXT20_002298"
     CDS             9227..9553
                     /locus_tag="IXT20_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAQ4860726.1"
                     /translation="MISSDGIRGYNDAIILSILNKNDSYGYEISKTIRERTNDSYVIK
                     ETTLYSVFARLEKNQLIVSYPGEVTHGKKRTYYKITEIGRNLLEEKKQEWLTTKDVVD
                     QFLEGN"
     gene            9554..10303
                     /locus_tag="IXT20_002299"
     CDS             9554..10303
                     /locus_tag="IXT20_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4860727.1"
                     /translation="MDTIRNYLDSLFIGVPQSTEIDKLKTDLLANMEDHYHELMGEGK
                     NEQEAIGTVISTFGSIDELLEELDVEKKHQADETETNTASIYLSEAENYWKEYRAASL
                     QVASGVLFISLSFASFLFFCSAGYVFMGISCLIFGIALAVGFFIASGMKITRLNHFLH
                     HRKIPEKVLAEAKEKEEEYQRSFGFSLIAGIGLCIFSLFPLLASLMWYMDGSIGASIF
                     FVTVGTGVFLIIYGSLVRHSYRQFTQSAYYW"
     gene            complement(10383..10838)
                     /locus_tag="IXT20_002300"
     CDS             complement(10383..10838)
                     /locus_tag="IXT20_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HAQ4860728.1"
                     /translation="MLTIYSSRTHSYHLMANWLKDHQMPYRERIFTPKQPLTRKEIFY
                     LLSLTENGFDDLLAKRSIDYKNLKDQIDQCTTSELVDLIVSHFTLLQRPIITDGKKLL
                     VGFNEDSMRLFIPVQWRRSQLKSVYNDFTWVSNLENSTDLDDEFLNYYI"
     gene            complement(11265..11540)
                     /locus_tag="IXT20_002301"
     CDS             complement(11265..11540)
                     /locus_tag="IXT20_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4860729.1"
                     /translation="MKLEDKKVILKKIKRLPDQIDGYTICSFKKDRSIKAYTQNTEKG
                     IIYFLHEEGYDTQTFMFTEKKEFHKQMKKLIEKEFPRSHKLYVNQQF"
     gene            11834..12649
                     /gene="thiD"
                     /locus_tag="IXT20_002302"
     CDS             11834..12649
                     /gene="thiD"
                     /locus_tag="IXT20_002302"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HAQ4860730.1"
                     /translation="MEDQYVQAMTIAGSDSDGSAGMQADMHTFFTRNVYGVSVMTACV
                     AGNSYGIGASVTLPTDFIDKEFELIAKDFQIRAAKTGMLADSKLIETVVKNYQKYDFG
                     PLVVDPVIVTKHGNLLLEESALQSLKEKLVPLAEVLTPNFYEAEKLTGRTLAAEEDFV
                     QAAKELQDMGAKNVMIKGHHAKNAQQETVNDYVLFESGKSFWLNAPFYPTDRINGTGD
                     SLSACIAAELAKGKTVEQAIRLAKDYVNRAIREEINVGHKYGPINHWAAGELE"
BASE COUNT         4055 a         2175 c         2587 g         3853 t
ORIGIN      
        1 aaaaaatgaa aaatcggtta attgtcgcat gtggaagtgg agtagcaacg agtcagacaa
       61 tcgctagtaa gatccaaagt atgttagaag atgacggcat cgcatttcct gtagaagcag
      121 ttgattacaa atcgattcaa aatgagcttc taactgctgg aatctatgtg tatgtcgcac
      181 aacctgatga ggaagtactt gaacaagcaa aagatttggg tattgaagta tttccaggaa
      241 ttccattttt aactggaatg ggtgtagaac ctatttatga cagtatcaaa gagttagtac
      301 agtaaaggag gattgttgga taaaaaagtt ggttcatagt tttatttact atatttagaa
      361 agaaagaagg tttatttatg gaagttttcc aaaccattat caattatgtt ttgaacttag
      421 gatcagccat ttttgttccc ctgattattt tgatacttgg attgttggca gggatgaaat
      481 taaaaaaagc attcatgtct gctttgactt tagggattgc ttttagcgga atgtctatgg
      541 tcattggcta tatgtctaac gcagtaagtc cagcatcaga agcattggct aaaaatacgg
      601 gaatcagttt gcctgcactt gatcttgggt ggacaggtgc agcaagcatc acatggtcgt
      661 ggagttatgc ttttatattt tttgcagtaa cgattggggt caattttgta ctgttacttt
      721 ttaattggac gaagacgtta aatgttgata tgtggaatgt ttgggggaaa gctttaactg
      781 cttatttagt ttattatgtt tccggtagtt tggctgcagg ctttcttact gcaatggtcc
      841 aagttatatt ggaattaaag cttggtgata tgtttcaaaa acatatacaa gatctcacag
      901 ggattccatt agtaacagtg actcatttta tgacatctgc cgctgtcttg ttgctgcctt
      961 tcaatatgat catggataaa attcctgctt taaataaacg agcggatacg aatgcattga
     1021 agaagaaaat tgggatcttc tctgaaaact ctgtaatggg attcattatt ggattggggc
     1081 ttggctttgc tgctgcgtat ggcgtttcag gttctcttaa tctagcgatc caaacagcta
     1141 cagcattaag tttgtttcct atgatctcta aaatgttcat gcaatctttg tctcctctag
     1201 cagacgctat gtcagaattt atgaaaaagc ggtttaagga tcgtgaagta tatatcggat
     1261 tggactggcc gatattagct ggtcgttcag aaatttgggt tactgctatt ttacttgttc
     1321 ctgtgtttat tggttatgcc attattttgc caggaaacca agtattgcca ttagcaggga
     1381 ttatcaacta ttcgattgcg gtcggcggat tacttttaac aggaggaaac ttggctcgta
     1441 tgctgatctt gggaatcatc actatgccta tttatttgta cggggccact taccttgcgc
     1501 caatactcag tgatttagca gaaaaaagcg gagcagtcga gggaatcaag aaaggacagt
     1561 tgatcacatg gtcatccata gaagggccgg agttcagggt tttatttgcg gaggcattta
     1621 ataggaatat tctcgctatt gtaggatgcg tgatttttct aggactgttt gttctacttt
     1681 ataaatatat gataaaagcg aaagtaccga gtgaacgtta tgctgaacta caaaaatagc
     1741 attgaaatgg tgggaaagta atgaaaagag aagagacaga taaaataaaa tggacagtcg
     1801 ctctttgtgg tacacttctg ctatttctat atggactctt tactcagaat atcatcatca
     1861 atctattggt catttttttc gcactagtga tctataaata tggaaatcac gttttattca
     1921 gagagtacga tgaaaaaaga aaacggaaaa tagaagaatc aatgaaaata aaagaagcta
     1981 ccaaagaaat attgagagaa aaaagtttta taaaaagata atggaggggt cattatgtca
     2041 gatggacgta tgttatttga gagagaacga caggacatgg cacgtatcgt aagagtgatt
     2101 ttcgaaagga aaaacacaaa tgtcgcagga gggaattttt cctttaaaac cactgatgat
     2161 caagggaaag actacatcat catgacgccg accatgatgt cagaagccta tctaggagag
     2221 atttctcctg cacaggttct agtagtagaa ccaagaacgc gtagaatcgt tgacggagtc
     2281 ggtcatttaa ctcgtgaaat caatatgcat gaagcgatgt atgagactaa tccagctatc
     2341 aaagctgtcc tacatgctca tgcgcctaat gcaatgttct gggcgactac tgggttgcct
     2401 atgcctaatt tgacagaagc cacagctaaa gtaaaagaga ttcctgtcat ggactttgag
     2461 cctaattgct ctgaagaact tgctgaattg acaagcgact atgtaaaaaa acagcatctc
     2521 gaattaccgc atatgttgct gctcaactct catggcgtat tgatcaacac aggtggagaa
     2581 tcaggaatcg aagctatcca taaagcactg caaatccttg atacagttga atggaatgca
     2641 gaaattgcct acaagcagac gatttttcaa gcattaggga ttactgacgg tttttattca
     2701 aaaggtgaga aaatcggggg attggaagag ttgaaacata aaacggctat ttataataaa
     2761 tctgttattg cagctggtgg agactaacct atctgacaca aaaaactacg actttcacaa
     2821 aatctgatcc agatcgatat ctgcttcagg ttgtattatg taagtcgtag ttcttttctt
     2881 ttatttagtt aagaggtggt ctgtccattc cctcgcttgc ttgatttcgt ccatcgtcaa
     2941 ctgatgacct gttacagtta gctgaatagt cacatttgct tggcgtgttt cgaattgatc
     3001 ggccaattct ttaaaggaag caggggaaac gattggatcg ttatcaccat aagaaatcca
     3061 gacggtttta tcctctaatg tggaacggct ttgatgaaca cctagagaca tcggatggaa
     3121 aaacaaccct ttatttagct cggtctcacg ctccaataat aaatgagcag cgatgtttgc
     3181 gccatttgaa tagccgacaa gcagccagtc acttaacggt atcagttctt tttcgctgat
     3241 ttctttgatt ttttcatgga gtttatctgt ttctttttcc aaactgtcca ggtcgaattg
     3301 attcaatcca ttacgcttga aaaaacgatt catcccatct tcttggaccg tgccacgaaa
     3361 agaaagcaat gtactgtttg gtgcaagaaa gtgagcgata tccagtaaag aatgttcatc
     3421 gccacctgtg ccatgaagta agatcaaatg cttgccactt gttccttttt caaaaatgta
     3481 ggtcattttg ttccctcttt tctgtttcta tagatgcaaa tgaagtagca tgaagagatt
     3541 attcacgttt tttatttgca tcacttgtat cgaaaggacg gacataagct tcaatggctt
     3601 cacgacgttc ttctaagaat ggaggaagag atagtttttc tcctgctgat tctgctggtt
     3661 catcttcaag gaatccagga ccatcagttg ctaattcaaa cagtacgtga tgagcggcta
     3721 ggaagtactc agattggaaa tagaaacgat cgacgaaacc agaatttggc aattcaatct
     3781 tattgattac ttgaatccag tattccaatg cttctttgtc tgctacacgc aatgctaagt
     3841 gatggatatt tccaaatcct tcgattgctt ttggcaagtc tgtccgatgt tcaataatga
     3901 tgcttgcacc atttccccct tcaccgattt caaataagta gaaatctcct gaatggtctg
     3961 tttctttgaa tcctaagatt ttttcaagaa tcaagcggat atgctctaat tgactgacac
     4021 ggacaaagac aggtccaagt cctgtgatcg caaactctgt cggtacattg ctgtttttcc
     4081 aaggaatgcc agctgctaca ccttgattgt tttcgtcaga gatcaattga taatactgat
     4141 tatcgaaatc ttcaaattcg atatattttt tgccgaaacg ttcattgatt gctgaatgag
     4201 taacgtcata tttttcaaaa cgttccaacc aaaagtctaa tgctgcgtca cttggcacac
     4261 ggaaagaagc acgagagatc gtatctgttc ctttttttcc ttttggctga tttggaaaat
     4321 caaagaaagt catatctgtt cctggcgcac cagtatcgtc tgtaaagtaa agatggtaag
     4381 tttcgatgtc atcttggtta actgtttttt tgattaagcg cattcctaaa atatctgtaa
     4441 aaaagcggta gatttttgta gcgctagatg tcattgctgt gacgtggtga attcctctga
     4501 tttgtgtatc catgataaat ttcctcctgt taacttacaa aaagtaagta gttgatttag
     4561 acatagtata gagggtgcat ctctattttg caattaattt gtcttctaaa gtatcaggaa
     4621 ggaatcaaga atggaaaaga ggagggacgg atcaataaaa taaaaagact tacgactagc
     4681 tgtatcgatt tcaaaaaaac tagctttaaa aagctaactt tagaatcatt acgggtggtc
     4741 ataagtcttt ttatgctgag ttattttttc atttttgagc taatgcttag gatagaatag
     4801 aatgttgatt tcacgatctc ttcttgttac ttgaaactgc ttaccactgt cacgatctct
     4861 tggtaaacgg ctttttcaca acctctttgg ttaagttgaa cgcacatcca gcacagaata
     4921 gttccgaaca tcaaatcggt ttcggctgga agaatattca atcggctgtc catctttgag
     4981 atagacgact tgctgaactt ccagtacagg atcgttttct gcacagttca aatattgctg
     5041 atcatattca tcacttttat ctgcagaaat agcacgataa gcccctgcaa aatggacatt
     5101 caattcttga tgaagatgat catagataga ggctaacaaa tgttcttttt ttaaacccgg
     5161 cacaagatgg actggcataa aggtatgctc taaaacatat ggctcgccat ctaagatcct
     5221 taaccggtga atattgtaga caggttgctc ggcatcgatc atcaatcgtt cttggatata
     5281 ttgattaggg aattcaacat caaaatgaat gatttgactc ttcaaagaac gatgtctgcg
     5341 tttcatttgg agactcaaac cttcatactc gttagcgggc gatgtgtctt tttgcataaa
     5401 tggatgattc aagattttcg ttcctgatcc tcgctgcgaa tagacaagtc cttccattgc
     5461 taagatattg atggcttttt tgattgtcat ccggctcgca ccaaattctt ttaccaagtc
     5521 gacttgatta ggcaaaaaac tatcgagagg atactctttt gaacggatac gttctcttaa
     5581 aatatttgcg atttcttcgt attttggcac gttattcacc tcttctagag tataacgaaa
     5641 gattatgctt ccgacaactc gaagtagcat ctggaacatt gtttattcga aaaaagaggt
     5701 catgacaagt ttttgtcaca accccttaat gatctattaa gcaatatcta aatctggttg
     5761 tttatctagt actttcagga atgggtacca gataaaggca acgatgacaa agtcgatgac
     5821 ttgcagcata ccaccaagcc atgaatttgt tgctaagaca ccgcttgcaa tgattggcac
     5881 agtccatgga acagagacgc cagttggtgc agggacaagt ccagcagcca ttaccaaata
     5941 attgaacgtc gttacgatta gaggtgccac aacccaagga ataaggattg ttgcattcaa
     6001 tacgatcggt agaccgaaga tcgctggttc atttacattg aaaataccag gtgctaaagc
     6061 taatcgtcca acatcacgat actgtttctt tttaagaaca aaggccatca ataaaacgac
     6121 agctaaagtc attccagagc caccaatacc aacagtgaag gtttccatga atggttttgt
     6181 gatgatgtgt ggtaattctt ttccatgttg ataagcttct aagttatcaa gcatcagtgt
     6241 attccaaatc gggtccatta cagagttgac gatgatttgt ccgtgaagtc cgaagaacca
     6301 taagaattgg acaaagaaca aagcaatcaa tgttgcaggt aaactacttc ccaaaccagt
     6361 taatggtttt tggatcactt cataaacgac gtcatgtaag ttagcattga aaataccagt
     6421 catcacagca ttgattacta agaaaatagt tagtgtcact acagcaggga tcaaagcggc
     6481 aaatgatttt gttactgcag gcggaactcc atcaggcatt ttgatctgcc agccgcgttt
     6541 cgtgattctg cagtagattt ctccagccaa gaaagcagcg atcattccaa tgaacattcc
     6601 ttttgcgcca agacgatcaa gtgaaagcac accagtcaca tcttcaccgc cagcggtttt
     6661 cattgcaaaa ggcgtaagaa tcaagaagct ggcaaaagac acggcaccac cgaaaatacc
     6721 ttcaacgtca taagattttg ataaataata gccgatacca aaagtaacaa aaaccgacat
     6781 gatgctcatt gttgcatttt gcccattacc gaataaattc gataaagttc cctttgttgc
     6841 gtcactgaaa aaaggcaagt tattgataac gacaacgatc gaaccgaaca tcgttaaagg
     6901 gaaggaaagc atgaaagcat cccgcagcac tgtaaggtaa cgattttgcc ctaataaatt
     6961 agctaagggc atgattttct cagaaagctt gtccataaaa ccgttcattt ttattcacct
     7021 caaatttatt tgcaatcgat tacatacaaa gtataacgcc aaaaaattta aatgtctagt
     7081 cattttcata aaaagtattg atttattttt tagggttcgt tatgattacg gtatcaagaa
     7141 aaaagagagg aagtaaaaaa atgaaaacct acacattccc agaaaatttt tggtggggtt
     7201 ctgctgctag cggaccgcaa acagaaggaa gagtaagtga tgatggtaaa ggagaaaata
     7261 tttgggatca ttggtatgaa ttagagcctg aaaaattctt cgatcaagta ggacctgaaa
     7321 aaacttcgca agtttacaca aaatacaaag aagatgttca gttaatgaaa gaaactggcc
     7381 ataacacatt tcgtacatct atccaatgga gtcatttgat tccagaaggg acaggtgaaa
     7441 tcaatcaaaa agctgtttct ttttatcgag atttttttac tgaattgctg gataatggta
     7501 ttgaaccttt tgtgaacctt taccatttcg atatgccact accactgcaa gaaaaaggcg
     7561 gatggttatc aagagaaacg gtcgacgctt atgtttctta tgcagcagcg tgctttgaac
     7621 tgtttggaga tctggtcaaa aaatggttta ctcataatga accaatcgta cctgtagaag
     7681 gaggctactt gtatcaattc cactatccaa atcatatcaa tatgaaagaa gcggtccaag
     7741 taggctatca tgagaatcta gcaagcgcaa aggtaatcaa agcctaccat gaaggcggac
     7801 atgatggtga gatcggaatc atcttgaatc tgacaccaag ctatccgaga gatgaaacga
     7861 acccagaaga tgtaaaagcg gcgaagatcg ctgacgcatt cttcaaccga tcattccttg
     7921 accctgcagt gaaaggagag tttccacaag agttgatcga aattgtcaaa gaattggcta
     7981 taatgccaga gatccatgaa ggagacctag aactcatccg tgaaaacacg attgacctgc
     8041 taggaatcaa ttactatcag cctcgtcgaa tcaaagcaaa agaaactcca atcaatcacg
     8101 aacatagccc catgccagat gattattttg ataattacga catgcccaat aaaaaaatga
     8161 atccatatcg tggatgggaa atttacgaaa aaggaatcta tgatatttta acgaatacaa
     8221 gagaaaacta tggtaatatc aaatgctata tttctgaaaa tgggatgggg gtcgaaggag
     8281 aagaacgatt tgttaacagc gaaggaatta ttgaagatga ttaccggatc gagtttgtta
     8341 cggatcattt gaaatacgtc catcaagcaa tccaagaagg aaccaattgt gtgggttacc
     8401 acatgtggac atgtatggac aactggtctt ggacaaatgc ttataagaat cgctatggct
     8461 ttatcgctgt tgatttggca caagacggta aacggacgat caaaaaatca ggtcgctggt
     8521 ttaaaacggt ctctgaaaaa aacggatttg atgcctgata ttttttgata aaacgttcac
     8581 aaaatgtttg ttctaacact gtaatcgtgc tacactaaca gtgaaggagg cgtttacaat
     8641 ggtagatact tatcgtaata ttttagtagc aattgatggt tctgaaaagg cagaaaaagc
     8701 atttcttgag gcaatcacga ttgccaaacg aaatcaggca acgttgcaca tcctgtatgt
     8761 caatgaagta acaggcaatt actttggtga ttttgcattt gttacaacaa gccttcagga
     8821 agaattggat gaggttgcgg aaaaccagat gaaagaacat cgaaacttgg caattgaaaa
     8881 aggcctgaca gatatcgaga catatgttct ttacggttat ccaaagacac tgattgctaa
     8941 tttccatgaa tcgaaagaac caatcgatct gattgtcatg ggggcgacag gtctgaatgc
     9001 ggtagaacgt gcactagtag gttccactac ttcctatgta gtcaaccacg catcatgtaa
     9061 cgtcatggtt gtaaaataaa taaattaagg gaacagaaag caaaaaagca taacgcttct
     9121 tttgctttct gttcctttta tttttttgta aaaaattact ttacctgatg aagtaatcat
     9181 gctattctaa ttacaacgac agatggagta atatggagga aagaaaatga tttcaagtga
     9241 tggaatacgc ggatacaatg atgcgattat tttatcgatc cttaacaaaa acgattctta
     9301 tggatatgaa atttctaaaa cgattcgtga gaggacaaat gattcctatg tgatcaaaga
     9361 gacaacactt tactccgttt ttgcccgttt agaaaagaat caactgattg tttcgtatcc
     9421 tggagaagtg actcatggaa aaaagcggac atattataaa atcactgaaa tagggcgtaa
     9481 tcttttagaa gaaaaaaagc aagagtggct gacgacgaaa gacgtagtcg atcaattttt
     9541 ggagggaaat taaatggata caatccgtaa ttatttagac agtcttttta tcggagtacc
     9601 tcaaagtacc gagatagata agctgaaaac tgatttgttg gcaaatatgg aagaccatta
     9661 tcatgaattg atgggagaag gaaaaaatga acaggaggcc atcggtacag taatttcgac
     9721 atttggttca atcgatgaat tattagaaga actagatgtc gaaaaaaaac atcaagctga
     9781 cgagacggaa acaaacacag cttctattta tttaagcgaa gcagaaaact actggaaaga
     9841 atacagagcg gcaagtctcc aagtggcatc aggtgttcta ttcattagct tatcttttgc
     9901 aagttttctt ttcttttgtt cagcaggata tgtgttcatg ggtatttctt gtctgatttt
     9961 cggtatagca ttagctgtcg gtttttttat tgcttctggt atgaaaatca cacgtttaaa
    10021 tcactttttg caccatcgga aaatacctga aaaagtatta gcagaagcaa aagaaaaaga
    10081 agaagagtat cagcgaagtt ttggcttctc actgattgca ggaattggat tatgtatttt
    10141 ttcacttttc ccacttttgg cttctttaat gtggtatatg gatggtagta tcggtgcctc
    10201 tatatttttt gttacggtag gaacgggtgt cttcttgatc atttatggca gtctagtccg
    10261 tcattcttat cgtcaattca ctcaaagcgc ttattactgg tgaatgtatt cagatttgta
    10321 aaaatattct atttatccgg cgtagaaaaa aagactgtga cacgcttttg tcacagtctt
    10381 ttttatatat aataatttaa aaattcatca tctaaatccg tagaattttc tagattgctt
    10441 acccatgtaa aatcgttata gacagatttc agttgacttc tgcgccattg tacagggata
    10501 aaaagacgca tgctatcttc attgaatccg actaataatt tctttccgtc agtaatgatg
    10561 ggacgttgta acaaagtaaa atgcgagacg atcagatcga caagctctga ggtcgtacat
    10621 tggtcgattt ggtctttcaa atttttataa tcaatcgagc gtttagctaa tagatcgtca
    10681 aatccgtttt ctgttaatga caataaataa aatatctctt tccttgttaa aggctgttta
    10741 ggtgtgaaaa ttcgttctct gtaaggcatt tgatggtctt tcagccagtt tgccatcaga
    10801 tggtaactat gtgtgcgaga agaatagatc gtaagcataa aaaaactcct taaatagtcc
    10861 attaatttgt tagatctgtt gagataaata ataggtaagg agctgtgcat aagcattgat
    10921 caaaacaacc caaacatcat tagaaataac tttctaaagt cttaaacttg cgacgagaaa
    10981 caactagact aatagaaaaa atgtcttagc catttggctt cctgtttgtt tttttgctta
    11041 tgctgcagct cctcgggaaa gggttctaac aatttgaaga aggttgatga ttgatctagt
    11101 ttaaattgtg tgaaatttga aatctggtgt ttttactaga tgaatcacct aacaactatg
    11161 attataactt atagaacttc tgttttgcat tcggttttgt tatagttgtt ataaagtttt
    11221 attttattac agaacatttt taattaataa agaacagtta gaagttaaaa ttgctgattc
    11281 acatagagtt tatgacttct tggaaattct ttttcaatca gttttttcat ctgtttatga
    11341 aattcttttt tttcagtaaa cataaaggtt tgggtatcgt aaccttcttc atgtaggaaa
    11401 tagattattc ctttttctgt gttttgcgta taagctttta tactcctgtc ttttttgaaa
    11461 gaacagatcg tatagccatc tatttggtct ggtagtcgtt tgattttctt caaaatgact
    11521 tttttatcct caagtttcat ttttttcacc tgactttatt tttttcatat ggaaatcata
    11581 gcgcctttgg ttatatgaaa acaagaagga tactgaaata tatataaatc aaatagtggt
    11641 tatttattat tagaatagcg aatacaaata aacgaccact attttctgat ttattgcaaa
    11701 tgttttttca aataaatttg attaccaaga aaaggtagtg gtatatttac tgagggatac
    11761 tttgaaaaac atatgataga aacagtaaca gtattatgaa aaatcttttt ataaaaatgg
    11821 aggaatcgat ttgatggaag atcagtatgt acaagcaatg acgatcgcag gatcagatag
    11881 cgatggaagc gcagggatgc aagcagatat gcatacattc tttacaagaa atgtttacgg
    11941 tgtttctgtc atgaccgcat gtgttgcagg taattcttac ggaatcggag caagtgtgac
    12001 attgccaaca gattttatcg acaaagaatt tgaactgatt gcgaaggact ttcaaatacg
    12061 tgcagctaaa acaggtatgc tagcagattc aaagttgatt gagacagttg taaaaaatta
    12121 ccaaaaatat gattttggac cattagtcgt ggatcccgta atcgtaacga aacatggaaa
    12181 tcttttacta gaagaatctg ctttgcaaag cttgaaagaa aagcttgtcc ctttggccga
    12241 agtactgaca cctaattttt atgaagcaga aaagctgact ggacggacat tagcagctga
    12301 agaagatttt gtgcaagcag caaaagaact gcaagatatg ggtgcaaaaa acgttatgat
    12361 caaaggacat catgctaaaa atgcgcagca agaaacagtc aatgactacg tattgttcga
    12421 atcaggaaaa tcattttggt taaatgcacc attttatcct accgaccgga tcaacggaac
    12481 tggcgattcg ctatctgcat gtattgcagc tgagttagcg aaaggaaaaa cagttgaaca
    12541 agcgatccga ttggcaaaag actatgtaaa ccgtgcaatt cgtgaagaaa taaatgtagg
    12601 gcacaaatat ggccccatca atcattgggc agctggtgaa ttagagtaaa agaaagaagt
    12661 tgtgaaaaag
//
LOCUS       DACGLE010000078        12268 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0534
            SAMEA3304449-rid9560453.denovo.082, whole genome shotgun sequence.
ACCESSION   DACGLE010000078 DACGLE010000000
VERSION     DACGLE010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304449
            Sequence Read Archive: ERR985064
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12268)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12268)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702225.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702225.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 20:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,045
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 27 of 67
            Pseudo Genes (incomplete)         :: 25 of 67
            Pseudo Genes (internal stop)      :: 19 of 67
            Pseudo Genes (multiple problems)  :: 4 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12268
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304449-rid9560453.denovo.082"
                     /strain="VREN0534"
                     /db_xref="taxon:1352"
     gene            complement(<1..570)
                     /locus_tag="IXT20_002303"
     CDS             complement(<1..570)
                     /locus_tag="IXT20_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001071399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAQ4860731.1"
                     /translation="MDQAKSLETACKLLRLSHVLPTLVRKDLDYSFETKEDWLMYLLE
                     KELACREELKTARLLKQAKFPNKRTLEDYEWHDHICLPNATTKDELLSLSFIHQKENA
                     VFVGTPGTGKTHLACGLGQLAAKSGIDTRFWRVSDLVNELEHHWKNNTLEVFKHRFDK
                     VKLIILDEMGYVPFTKEGAELLFQLISDWY"
     gene            complement(570..1994)
                     /locus_tag="IXT20_002304"
     CDS             complement(570..1994)
                     /locus_tag="IXT20_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000957075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HAQ4860732.1"
                     /translation="MLTMTDINTIRNLRNNHDKSINHIAKELDVNWRTAKKYADDDLL
                     PQPKARKKTGMMYTEKWGGIVSLWLSEDAKLPKKKRRTAEAMTTALKADGFPGSYRTV
                     CVFIQDWKATHYLETLEDQGFERLEHPPAEAQLDFGTMEVCHDGAFKDVKVLVMTFPF
                     SNAGFAVVLPAENQECLLEGMKELFSQSGGVPRKIRIDNMSTAVTQTKTKTEQAVLTD
                     GFLRFSMHYGFETQVCNPRSGNEKGSMENKVGYVRYNFFSSTPIMKDFASFNEALAVE
                     LSHDRERIHYEKHVIINDLWNTEKTDLLALPDTDYPVFKEIEVKVNKYNEIKLDNERI
                     HVPRVRNYSIIYGLLRFDSYQLISTDGEIISEGLRPYMMKKRAIDWQAILPDWRRKSR
                     AMHYSRYWKYLPERIKLFLSHPDWKEQEKRLNQISGLLVTHDMLGIDTHFYDMFSAST
                     TTDAYDIDWHQYDAFLSLTGEVID"
     gene            complement(2389..3231)
                     /locus_tag="IXT20_002305"
     CDS             complement(2389..3231)
                     /locus_tag="IXT20_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HAQ4860733.1"
                     /translation="MKGDVIVEIKVLFENSRINLNFKSGHGLSLLINYSGKKYLFDAG
                     ANKKLVYNATKMGETLNDLEAVIISHGHSDHTGGLRYLKSGNAPIYTAEGVTLPHYLK
                     ILTTFLYVGIPKNEILKMHENLHTIKTKTELAPSFHIIPLGKANSTTKNLYKKMDDKF
                     VYDDFSDELLVVIEQEDGLIVFTGCSHHGIIDMMEIALETFPTKPIKYLIGGFHMIGI
                     PYINNLGLPEQEVMKIGNKLNDSRVEKILTCHCTGSKAFNILKTQLGNKLEAIKTGQH
                     LEIS"
     gene            3556..3972
                     /locus_tag="IXT20_002306"
     CDS             3556..3972
                     /locus_tag="IXT20_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HAQ4860734.1"
                     /translation="MATIILASHGEFAQGLKQTATMIIGDSVPIHALSAFRDEDESIL
                     VQIKKLLATIDIEETYILTDILGGSVNNDMLTIIKEYEGLQLITGMNLPLVISIATYN
                     GKIGESELEKLIRESQQSVIDCRKLLVEQLTGGDDL"
     gene            3969..4439
                     /locus_tag="IXT20_002307"
     CDS             3969..4439
                     /locus_tag="IXT20_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016622974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ4860735.1"
                     /translation="MIKLVRIDHRLLHGQVVFSWSKSLNINRIIIANKEAANDEFKKM
                     SLNLSKPTGIKLTIFSVEQLLEKMPKIETLNENIMLIFGNTKEIRQFCEAYPKVQEIN
                     YGGIIKKEGSKQFSNAIFLNESEQEDAKKLKALGIKQFIQQVPTSKKEDLNTML"
     gene            4453..5226
                     /locus_tag="IXT20_002308"
     CDS             4453..5226
                     /locus_tag="IXT20_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ4860736.1"
                     /translation="MLVQSIGLGLIGVFCILDSRLLGRMNFERPLIVSTLVGLVLGDM
                     EKGLIVGASLELMSLGLVNIGAAAPPDMNMASIIATAFAILSNANAEAALTIAIPISV
                     LGQILGILMRTILSNLTHVADRFIEQGKFSQARRIHILYGSGLYALMYFIPIFLAIYF
                     GTNLVSQIVDAIPEWLTNGLTVASKILPAYGFALLLQTMLTKKMVPFLLLGFLITAYS
                     GLGIIGIAAFACIVAFVMVQVQGKNSNGNQNEPDPLDDL"
     gene            5240..6052
                     /locus_tag="IXT20_002309"
     CDS             5240..6052
                     /locus_tag="IXT20_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAQ4860737.1"
                     /translation="MKEKDQNAWKYRQFLWRSWAIQASWNYERQMNMGFMYGIAPILD
                     NIYKNPEDEAMKKEAYERHMMYYNCTPQTSAFVLGLTASMEEQYYEDQENFNPESISA
                     VKTSLMGPLSGVGDSFFQGTIRVLAFGLGINLAQQGSILGPILAMLISFVPSFLVTYY
                     GGKIGYNMGNKYLSKLYQEGLMDKVMYVCSIVGLMVIGSMIASMIGITTPIQMGKAFV
                     LQDVLDSIMPQFLPLGLTFFMYWLLQKKVKTGWMLAICLVGGIIFSLLGILQ"
     gene            6106..7071
                     /locus_tag="IXT20_002310"
     CDS             6106..7071
                     /locus_tag="IXT20_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAQ4860738.1"
                     /translation="MNSKEIVKEIVAKKEVKSIVFVGCGASKADLYPAKYFLDQNAKQ
                     LRISHYTASEFNYATPASVDESTVVISASLGGTTPETVEANAVAKEKGATVISLTRSI
                     DSPLTKDADYVIYHRFAENYAAKLEKTGYCLELAVELLHQVEGYDHYEAMQEGFATIY
                     DLAQKSAESARKDAKKFAERFKDEKVIYVMSSGATHEVAYSTSICLMMEMQWINSGTF
                     HSGEFFHGPFEIVDKDVPFILLMNDGKTRNIDARALTFLERFDTKTEVVDALDYGLSS
                     HIAKEVLDYFNPFVITAVFRVYAEELSEAREHPLTKRRYMWKLTY"
     gene            7208..9931
                     /locus_tag="IXT20_002311"
     CDS             7208..9931
                     /locus_tag="IXT20_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ4860739.1"
                     /translation="MQRLNDYLYQLTLLLKSTPSDNYSARSISQALQLNRSTISSYLN
                     EGVREGLFIKVKSYPVLFLHRTALEELHITLNNSEIESIESLLTISQKPALDQVIGSK
                     GSLKEAIDQIKTAVLYPGKGLPLLLIGASGSGKTFLANKIYEYAVEEKVIHTSAPFID
                     YNCAQYVSNPELLSSALFGYTKGAFTGASQEHTGLLEKADGGVLFLDEVHRLSEEGQE
                     KLFTFMDTGEFSPMGDNSIRKKADVRLVFATTENIYTTFLPTFLRRLPVIVNLPRFQQ
                     RPSFERLSLIDEFFVSESKILAKELSVSDALIHFLMNSDFEGNVGNIKNIIKYACGNA
                     YIHQKSQQTIQVKLLDMPLEYSYKFKEQLNKPRKKRADRIYYPEETRQVQLERNNQLF
                     VQFFDQLLTGFSEVIENNKDIKLFLEEMVTKVTQVMDTLIFQEDYEKEQSLYAILNYH
                     IRQSIDFMRESYGFEQDGNRVVALANYLYVKDNQVIFENSLQEKKTKDQLNEFFANVL
                     ETPYWYAKKILSLLSECLDQPVYEEDIFFITLYFYSLNVTNVKTGIKSIVLAHGYSTA
                     SSMANVANRMLNKNVFQAYDMPLDITLEKIEAKLIQFLGDYRTDSGLILLVDMGSLNQ
                     LGGLLVNHLNGPLLIIDHINTPLLLEVGQQVLNNKSITEIGAEIETSLTFKKQLLLPK
                     KKKKKAIITCCYTGIGSATQIQEILTKCLKDAVRELVIIPYDYKKLLENKNFETPFQL
                     YDVIMIVGTEDPKINQIPYIGLDRLINGEAIGEFVHYLEKELTIEVNDLQKELIFNFS
                     ISKIVENLTILDPDKVLSFVQKAIKELSKLLNFDFSNNQLFLLYLHCSCMIERIVRKE
                     SVDEQEDIEEYMQANGEIMDKIHSAFFEIEREYTIEIPDLELRLLSDIVQG"
     gene            9944..10576
                     /locus_tag="IXT20_002312"
     CDS             9944..10576
                     /locus_tag="IXT20_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAQ4860740.1"
                     /translation="MTMGVIFDMDGVLIDSETFYFERRMNFFQELEIDPGSNQIDDYV
                     GKTEAAIWETLVPEDKSLREELYPKYVDYRKTHPIDFAKDLRKEVKPLLDFLKKRDIP
                     LALASSSPVVEIESMLIQCELTEYFSFVISGESLKQSKPHPEIYLKAKQQLDCDFFIA
                     VEDSTIGIKSAKAANIYTVALKQPMQIDQSEADIQIESLQELEQIVLEKG"
     gene            11475..>12268
                     /locus_tag="IXT20_002313"
     CDS             11475..>12268
                     /locus_tag="IXT20_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HAQ4860741.1"
                     /translation="MKNLQLKFIVDPSIVRWFRIINQFERNQLATKGELAKSNHVSER
                     TIQTDVNKMKDYFSESVQFVSTKSGYSFQKERPVLFLKQKEQLLENEILFELLGNVFY
                     GELEDMTELIDRFHLSEATFRRYLKQIQPVLEEYGLELSLYPLDLKGEEANIRKFFKD
                     FYYEGEMTPHTLVPPRDLHELVQATFYDKFEYFSIGTGTSPAEFYYSLYIAIERVRQG
                     KTIAIPQGLIERVIASENFRLMYSLKEAIQTNYQVALSEEEFCWLY"
BASE COUNT         3977 a         2025 c         2378 g         3888 t
ORIGIN      
        1 ataccaatcg ctgatcaatt ggaaaagcag ttccgcgcct tctttagtga aaggcacata
       61 acccatctca tcaagaatga ttaattttac tttatcgaag cgatgcttga agacctctaa
      121 ggtattgttt ttccaatggt gttccaattc gttgactaaa tctgataccc gccaaaagcg
      181 tgtatcgata cctgatttgg ctgccagctg acccaatcca caagctagat gtgttttacc
      241 ggtaccgggt gtacccacaa atacagcatt ttctttctga tgaataaacg atagactcaa
      301 taattcatct ttagtcgtcg catttggcaa acatatatga tcatgccatt cgtaatcctc
      361 taaggtacgt ttatttggga actttgcttg ttttaggagt cgggcagttt tcaattcttc
      421 acggcaagcc aactcctttt ccagaagata catcaaccaa tcttctttgg tttcaaagct
      481 gtaatctaag tctttgcgaa ccaaagtggg aagaacatgg gaaagtctca gcaacttaca
      541 ggcagtttct aaactttttg cttgatccac tagtcaatga cctcccctgt tagagacaag
      601 aaagcatcgt attggtgcca atcaatatca tacgcatcgg tagtagtcga ggcactaaac
      661 atgtcgtaaa aatgtgtatc aatccctaac atatcatgtg tcaccaataa acctgagatt
      721 tgatttaaac gcttctcctg ttctttccaa tctggatgac tgagaaataa cttgattcgt
      781 tcgggcaaat acttccaata gcgagagtaa tgcatggcac gcgattttct gcgccaatca
      841 ggaagaattg cttgccaatc gattgctcgt tttttcatca tataaggacg aagtccttct
      901 gaaataatct caccatcagt gctgattagc tgataggaat caaaacgaag cagcccataa
      961 ataattgaat aattgcgtac acgtggaacg tggatccgtt cgttatctaa tttaatttcg
     1021 ttgtacttat tgactttgac ttcgatttct ttaaatactg ggtaatcggt gtccggcaaa
     1081 gctagcaaat ctgttttttc tgtgttccat agatcattga taatgacatg tttttcgtaa
     1141 tggattcgct ctcgatcgtg tgacagctcc accgctaatg cctcgttgaa actggcgaaa
     1201 tccttcataa tcggtgtaga tgaaaagaag ttgtaccgta catagccgac tttgttttcc
     1261 atactacctt tctcgtttcc gctacgtgga ttgcacacct gtgtctcaaa accatagtgc
     1321 atagaaaatc ggaggaaacc gtcagtcagc accgcttgtt cagtctttgt ttttgtctga
     1381 gtaacggccg ttgacatatt atcaatccgt attttccgag gaacgccacc tgattggctg
     1441 aagagttctt tcatgccttc caacaaacat tcttgattct cggcggggag tacaacggcg
     1501 aatcctgcat tactaaaagg gaaagtcatc actaatacct tcacatcttt aaacgctcca
     1561 tcgtgacaaa cttccatcgt accaaaatca agttgcgctt ctgctggcgg gtgttcaaga
     1621 cgttcaaagc cttggtcttc cagtgtttcc aagtagtgtg tcgctttcca atcttgaatg
     1681 aatacacaga ctgttcggta ggatcctgga aagccatcgg cttttaaagc agtagtcatt
     1741 gcttcagcag tcctacgctt tttctttggc agcttagcat cttctgaaag ccatagggaa
     1801 acgatgcctc cccacttttc tgtatacatc ataccagtct ttttgcgtgc tttaggttgc
     1861 ggcagcaaat catcatctgc atatttttta gccgtgcgcc aatttacatc tagctccttt
     1921 gcaatatgat taatggattt atcatgatta tttcgtaaat ttctgatagt attaatatcg
     1981 gtcattgtga gcataccttt cttacctcca gttacagtgt cgagactata actttagagg
     2041 aatttcaggt tgcccgcaat ggctttttat cttaacttaa tcgatttgcg gagttgtgta
     2101 gtttttcgtt gcacttgtat gtacttttat tctgccataa acagcttaat gaatgtccac
     2161 ttgaaaaagt gaataaacga aatagatgac tcgatttaat aaggaagtaa acccacacag
     2221 aaatataggt gattttcctt gaatgaccaa gatattgaaa gttagtgaaa aaacacaaaa
     2281 aggtagccct attcacagaa ggtctgcctt ttgtgttatc gccagcaaac attagagggg
     2341 atatttcata tagcttttgt cctctcgaca gtcctatcag tttctctttt aacttatctc
     2401 taagtgctgt ccagttttaa tagcttctag cttgtttcct aactgtgttt ttaatatgtt
     2461 gaaggctttt gaacctgtgc agtggcaagt aaggattttt tctactcgtg agtcattcaa
     2521 tttatttcca attttcatta cctcttgctc aggtagacct aaattattta tataaggtat
     2581 gccaatcata tggaagccac cgattaggta ttttatgggt ttagtcggaa atgtttcaag
     2641 agcaatttcc atcatatcga taatgccatg atgactacag ccagtaaaga caatcagacc
     2701 gtcttcttgc tcaattacga ccaataattc atctgaaaaa tcatcataaa caaatttatc
     2761 atccatcttt ttgtataaat tcttggtggt cgagtttgct ttgcctaggg ggatgatatg
     2821 gaagctcgga gcaagttctg ttttcgtttt tattgtatgt aaattctcat gcattttaag
     2881 gatttcattt ttaggaatac caacatacag aaacgtggtt aaaatcttta aataatgggg
     2941 taaagttaca ccttcagctg tataaatagg ggcgtttcct gattttaaat atcttaaccc
     3001 gccggtgtgg tcagaatgac cgtgtgaaat aatcactgcc tccagatcat tcaaagtttc
     3061 ccccatttta gtcgcattat agactaattt tttattagcg ccagcatcga agagatattt
     3121 cttccctgag tagttaatta agagagataa cccatgtcca cttttaaagt tcaaatttat
     3181 tcttgagttt tcaaataaaa ccttgatttc cactataaca tctcctttca catgaacgtg
     3241 tattcatata ttgtagcaca ttaagttcta ttttttgttg taattaccga aaaaaatatt
     3301 aatggacgct ttttacgctt tatttcgtat gtggaagaaa atttcttagg atgtggcggg
     3361 ggattggggc aaagctaaat tccaattaca gtgatcattt aattagacac ctttaaatac
     3421 ccacactttc atcaacacta taatttaggg aaagaaaagt gttggcatga tatagaaaaa
     3481 cctttaattg acagcgtttc ctaaaattgg cacgcatctt gcttatagaa aagcgtaagg
     3541 ataggaggaa tgaaaatggc aactataatt cttgccagcc atggggaatt tgctcaagga
     3601 ttaaagcaaa cagcgacaat gattattgga gatagtgtac caattcatgc cttgtcagca
     3661 tttagagatg aagatgaatc tattttggta caaataaaaa aacttttagc cacaatagat
     3721 atagaagaaa cctacatttt aactgatatt ctaggtggta gtgtgaataa tgatatgctc
     3781 actattatta aagagtatga gggattgcaa ttaattactg gaatgaactt acctttagtt
     3841 atttctattg caacctataa tggaaaaatt ggggaaagtg agctagaaaa gcttattaga
     3901 gagagtcaac aatcagtgat tgattgtaga aaattattag tagaacaact tactggagga
     3961 gatgacctat gattaaatta gtacgtattg accatcgttt gttgcacgga caggtagtat
     4021 tttcttggag taaatcatta aatatcaatc gtattattat tgccaataaa gaggcagcga
     4081 atgatgaatt caaaaaaatg tctttgaatt tatctaaacc gactggcatt aaattaacaa
     4141 ttttttcagt tgaacagtta ctagaaaaaa tgcctaaaat cgaaacactt aacgaaaata
     4201 tcatgcttat ttttggtaat acaaaagaaa ttcgtcaatt ttgtgaagcc tatcctaaag
     4261 tacaagaaat caactatgga gggataatca aaaaagaagg ttccaagcaa tttagtaatg
     4321 ccattttctt gaatgaatct gagcaagaag atgccaaaaa attaaaagct cttggcatta
     4381 aacaatttat tcaacaggta ccaacttcta aaaaagaaga tttgaataca atgttataaa
     4441 ggaggaatga aaatgctcgt tcagtcaatt ggtttagggt taatcggtgt tttttgtatt
     4501 ttagactcaa ggcttttagg aagaatgaat tttgaacgac cgttgattgt cagtacgctt
     4561 gttggtttgg tacttggaga catggaaaaa ggattaattg ttggagcctc tttggaattg
     4621 atgtccttag gattagtaaa tatcggagct gctgcaccac ctgatatgaa tatggcatca
     4681 attattgcta cagcctttgc cattttatca aatgcgaacg cagaagcagc attaacgatt
     4741 gccattccaa tttcagtgtt aggtcaaata ctaggtatct taatgcggac gattttgtca
     4801 aatttgactc acgttgcgga tcgctttatc gaacaaggga aatttagcca agcaaggaga
     4861 attcatatac tttatggctc gggattatat gctctcatgt attttattcc tatttttcta
     4921 gcaatttatt ttggaacgaa tcttgtttct cagattgttg atgcgattcc tgagtggcta
     4981 acaaatggct taacggttgc aagtaaaatt ttaccagcat atgggtttgc gttgttatta
     5041 caaacgatgt taacgaaaaa aatggtgcca tttttattac ttggattttt aattactgct
     5101 tattctggat tgggaatcat tggtattgca gccttcgcat gtattgttgc gtttgtcatg
     5161 gttcaagttc aaggtaaaaa ctcaaatggt aaccaaaatg agccagatcc attagatgac
     5221 ttataaggag gtagattaag tgaaagagaa agatcaaaat gcatggaaat atcggcagtt
     5281 cttatggcgt tcatgggcaa ttcaagcctc atggaactat gaacgacaaa tgaacatggg
     5341 ttttatgtac ggaattgcac caattctaga taacatttac aagaatccag aagatgaagc
     5401 aatgaagaaa gaagcttacg agcggcatat gatgtactac aactgtaccc ctcaaacaag
     5461 tgcatttgtg ttagggttaa ctgcttctat ggaggagcaa tactacgaag accaagaaaa
     5521 ctttaatccg gaatcaatca gtgcagtgaa gacttctctg atgggaccgc tatctggtgt
     5581 tggtgattct ttctttcaag gaacgattcg ggtattagcc tttggactag gaattaattt
     5641 agcccaacaa ggtagtattt taggtccaat tctagcaatg ttaatttcct ttgtaccttc
     5701 ctttttagtt acttactatg gtgggaaaat tggctataac atggggaata aatacttgtc
     5761 taaactgtat caagaaggtt tgatggataa agtaatgtat gtttgctcca ttgtggggtt
     5821 gatggtcatt ggttcgatga ttgccagcat gatcgggatt acaacaccaa ttcaaatggg
     5881 taaagctttt gtcttacaag atgtattaga tagtattatg ccacaatttt taccattagg
     5941 attaaccttc ttcatgtact ggttattgca gaaaaaggtc aaaacaggat ggatgttggc
     6001 aatctgttta gtcggaggaa ttatttttag tttgttaggt atcttacaat aagggaataa
     6061 ccaataaatt tggttgaata atataaataa ggaggaattc aattgatgaa ttcaaaagaa
     6121 attgttaaag aaatcgtagc gaaaaaagag gtaaaatcaa tcgtatttgt tggctgtggc
     6181 gcttcaaaag cggaccttta tcctgcaaag tattttctag accaaaatgc gaagcagtta
     6241 cggattagtc attacacagc cagcgaattt aactatgcaa caccagcatc tgtagatgag
     6301 tcaactgtcg ttatttcagc ttctttagga ggtacgactc cagaaacagt tgaagctaac
     6361 gcagtagcta aagaaaaagg tgctacagtt attagtctaa cacgaagcat tgactcaccg
     6421 ttaacaaaag atgcagatta tgtaatttat catcgatttg cagagaacta tgctgctaaa
     6481 ttagaaaaaa caggctactg tttagaattg gcagtagaac ttttacatca agtagaaggt
     6541 tatgaccatt atgaagcaat gcaagaagga tttgctacta tctatgatct tgcccaaaaa
     6601 agtgcagaat cagctcgtaa agatgcgaag aaatttgctg aacgctttaa agatgaaaaa
     6661 gttatttatg taatgagtag tggtgctaca catgaagtag catactcaac atcaatttgc
     6721 ttgatgatgg aaatgcaatg gattaattct ggtaccttcc attcaggaga atttttccac
     6781 ggaccattcg aaattgtgga taaagacgta ccgtttatat tattaatgaa tgatggaaaa
     6841 actagaaata tagatgctcg agcattaact ttccttgaac gctttgatac gaaaacagaa
     6901 gttgtagatg ccttggatta tggcttgtct tcacatattg ctaaagaagt cttagactac
     6961 tttaacccat tcgtgattac tgcagtattt agagtttatg cagaagaatt gtcagaagca
     7021 agagaacatc cattaactaa gagacgttat atgtggaaac taacttatta aaaaatagat
     7081 caaactaaat aaggtggctt gagaaattaa gccaccttgt tttcttcaat gtgattttat
     7141 acatatggta aaataactag tacatatggt aaaataacta gtacatattg tgaaagcagg
     7201 tggcaagatg cagcgattaa atgactatct ttatcaacta acattgcttt tgaaatctac
     7261 gccttcagat aattattcag ctcgcagtat ttcgcaagct ttacagttga atagaagtac
     7321 aatcagtagc tacttaaatg aaggtgttcg tgaagggctt ttcataaaag taaaaagtta
     7381 cccggtttta tttttacata gaacagctct tgaagaacta catatcacac tgaataactc
     7441 cgaaatagaa tcaattgaaa gcttgttaac aatttctcaa aaacccgcat tagatcaagt
     7501 aattggctct aaaggaagtt tgaaagaagc aattgatcaa attaaaacgg cagtcttata
     7561 tcctggaaaa gggttgcctt tattattgat cggcgctagt ggatcaggga aaacctttct
     7621 agcgaataaa atatatgaat atgcagtaga agaaaaggtc attcacacat cggcaccttt
     7681 catagattat aattgtgcac aatatgtgag caatccagag ttactatcct ctgctttatt
     7741 tggctacacg aaaggagcat ttaccggagc aagccaggag catactggtt tgctagaaaa
     7801 agccgatgga ggagtattgt ttttagatga agtgcatcgc ttatctgaag aagggcaaga
     7861 gaaattattt acatttatgg atacgggcga attctctcct atgggggata atagtattcg
     7921 gaaaaaagcc gatgttcgat tagtctttgc aacaacagaa aatatatata cgactttctt
     7981 accaactttt cttaggagat tgccagttat tgttaatcta cctagatttc aacaacgtcc
     8041 gtcgtttgaa cgtctgtcat tgattgatga attttttgtt tctgagagta aaattttagc
     8101 taaggaacta tctgtttctg atgcattgat tcatttctta atgaatagtg attttgaagg
     8161 taacgtgggg aatattaaaa atattatcaa gtatgcttgc ggaaacgcat atatccacca
     8221 gaagagtcag caaacaattc aagttaaatt attggatatg cctttagagt acagttataa
     8281 attcaaggag caactaaata aacccagaaa gaaaagagca gatcgaatct actatccaga
     8341 agaaacacga caagtgcaac tagaaagaaa caatcagctt tttgttcaat tttttgacca
     8401 attacttact ggattctctg aagtgattga aaataacaag gatattaaac tatttttaga
     8461 agaaatggtt accaaggtaa cacaagtaat ggatacgctt atttttcaag aggattacga
     8521 aaaagagcag tctttatacg ccattctaaa ctatcatatt cgtcaaagta ttgattttat
     8581 gcgagaaagc tacgggtttg aacaagatgg taaccgggta gtcgctttgg ccaactatct
     8641 atatgtcaag gataatcaag tgatttttga aaatagtctt caagaaaaga aaaccaaaga
     8701 tcagcttaac gaattttttg ctaatgttct tgaaacccct tattggtatg caaaaaaaat
     8761 cttaagcctt ttatctgagt gtttagatca acctgtgtat gaagaggaca ttttctttat
     8821 tacgctatat ttttatagtt tgaatgtcac gaatgttaaa acaggtatta agagtattgt
     8881 gctagctcat ggttattcta cagctagtag tatggcgaat gttgccaacc gtatgctgaa
     8941 taagaacgtt tttcaagctt atgatatgcc tttagatatt acattagaga aaattgaagc
     9001 aaaattgatt caattccttg gagattatcg aacagattca ggcttgattt tattggtaga
     9061 tatgggttca ctaaaccaac taggcggttt attagtgaat catctaaatg gaccactgtt
     9121 aatcattgat catatcaata caccgttatt attggaagtc ggccaacaag tattaaacaa
     9181 taaaagtatc accgaaatag gagcagaaat tgaaacaagt cttactttca aaaagcagtt
     9241 actacttccc aagaaaaaga aaaagaaagc catcattact tgttgttata cagggattgg
     9301 tagtgcgacc cagattcagg agattttaac gaaatgcttg aaagatgcag ttagagaatt
     9361 agtaattatt ccgtatgatt acaaaaagct gttagaaaac aagaattttg agactccttt
     9421 tcaattatac gatgtcatca tgattgtagg tacagaagat cccaaaatta atcagattcc
     9481 atatattgga ttagatcgcc tgattaatgg ggaagcaatt ggcgaatttg ttcattactt
     9541 ggaaaaagag ctgactattg aagtgaacga tttacaaaaa gaattaattt tcaatttttc
     9601 aattagtaaa attgtcgaaa atttaaccat tttggatcca gataaggtgc tatcttttgt
     9661 acaaaaagca attaaagaat tatcgaagtt attaaatttt gatttttcta ataatcagct
     9721 gtttttgttg taccttcact gtagttgtat gattgaacga attgtacgta aagaaagtgt
     9781 ggatgagcaa gaggacattg aagaatatat gcaagcaaat ggagaaataa tggataaaat
     9841 tcatagcgct ttttttgaga ttgaaaggga atacacaatt gaaataccag atttagagtt
     9901 gcgattattg agcgatattg ttcaaggata ggagtgaatt cgaatgacaa tgggagtaat
     9961 ttttgatatg gatggggtac tcatagacag cgagacattt tattttgaga gaagaatgaa
    10021 cttctttcaa gaactggaga tagatccagg atcaaaccag attgatgatt atgtggggaa
    10081 aacagaagct gcaatatggg aaacattagt tcctgaggac aaatcattaa gagaggaact
    10141 ttatccgaaa tatgtagatt atcgaaaaac gcatccaatt gatttcgcta aagatttacg
    10201 aaaagaagtg aaaccgttac ttgacttcct caagaagcgg gatataccat tggcactggc
    10261 atcttcttct ccagttgttg agatagagag tatgctcatt caatgtgaac ttactgaata
    10321 tttttccttt gttataagtg gtgagtcatt aaaacaaagc aaaccacatc ccgaaatata
    10381 tttaaaagcg aaacagcaat tagactgtga cttctttatt gcagtagagg attcaacgat
    10441 tggtattaaa tcagcaaagg cagcaaatat ctatacggtg gcactaaaac aaccaatgca
    10501 aatcgatcag agtgaagcag acatacaaat tgaaagtcta caagaactgg aacaaattgt
    10561 tttagaaaaa ggttagtgaa ttaatggaaa taaggctata tatctgtgct agctattttg
    10621 actaaccaaa aaaacttgct ataattcagg tgtagtaaaa ggtgctggta acacctttta
    10681 cgaaaatgct tcggcagtga taatgggatt ggctttttaa gttttttatt ttgctcgcaa
    10741 tccctaaggt catgtgaatg atcctaggga ttgctagaat attttgcaac attctatcta
    10801 tttcagaacg taaccttccc cagttcattg ggattcatta gcatacaacc aacatttcaa
    10861 aaagaaattg cactacacca ttattagcta ttttgactaa ccccaaaaaa cttgctataa
    10921 ttcaggtgta ggtaaaaggt gctggtatca ccttttacga aaatgcttcg gcagtggtaa
    10981 tgagtgtggc ttaaaggact agtcctttaa gctttttact ttgttcgcaa tctctaagat
    11041 caagttaatg atcgataaga ttgctagaat attttgcaac attctatttc agaacgtaac
    11101 ctttcccatt ccactgtgat tcattggcaa ccctcctcac aaccaacgtt tcaaaagaaa
    11161 ttgtaaagtt gttggggtac cacgaccgaa caatactaca cctgaatgat agtaagttgt
    11221 ctcattaagt atagcatatc ctctttttgg gtaaatagaa atcggttaca ttttttatcc
    11281 aagaccaagt tttttcattt ggtcttattt gctataccca aacaattccg cagtatagtc
    11341 gacggtattg attttatcag gtgagtaata gaaacttgtt ttccttcccc aaaacattac
    11401 ctataaagga gaaaaaatgg tatcgtaatt atataggtat aggatgaaat ttttttctaa
    11461 gaggagggtt gttgatgaaa aatttacagt taaaatttat tgttgatcca tcgattgtac
    11521 gctggtttcg gattatcaat caatttgagc gcaatcaact tgccacaaaa ggagaattag
    11581 caaagagtaa tcatgtatct gaacgaacca ttcaaacaga tgtcaataaa atgaaggact
    11641 acttttctga aagtgttcaa tttgtttcaa cgaaaagtgg gtattctttt cagaaggaaa
    11701 gaccggttct ttttctcaag cagaaggagc aactactgga gaacgaaatt ttatttgagt
    11761 tgttagggaa tgtcttttat ggcgaattgg aagatatgac ggagctaatt gatcgttttc
    11821 atttatctga agcgactttt cgtcggtatt tgaagcagat tcaaccagtt ttggaagaat
    11881 acggcttaga attgagtctt tatcctcttg atttaaaagg agaagaggca aatattcgca
    11941 agttttttaa agacttctat tatgaggggg agatgacgcc ccatactctt gttccaccaa
    12001 gggatcttca tgaacttgtg caagctacat tctatgataa atttgagtat ttctcgattg
    12061 gaacagggac ttctcctgct gaattttatt atagtttgta tattgcgatt gagcgtgtga
    12121 gacaaggaaa aacgatagcc attcctcaag gtctgattga gcgagtaatt gctagtgaga
    12181 attttcgctt aatgtattcc ttgaaagaag cgattcaaac aaattatcaa gttgctttat
    12241 cagaagagga gttttgttgg ttgtattt
//
LOCUS       DACGLE010000079        11903 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0534
            SAMEA3304449-rid9560453.denovo.083, whole genome shotgun sequence.
ACCESSION   DACGLE010000079 DACGLE010000000
VERSION     DACGLE010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304449
            Sequence Read Archive: ERR985064
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11903)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11903)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702225.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702225.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 20:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,045
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 27 of 67
            Pseudo Genes (incomplete)         :: 25 of 67
            Pseudo Genes (internal stop)      :: 19 of 67
            Pseudo Genes (multiple problems)  :: 4 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11903
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304449-rid9560453.denovo.083"
                     /strain="VREN0534"
                     /db_xref="taxon:1352"
     gene            complement(57..659)
                     /locus_tag="IXT20_002314"
     CDS             complement(57..659)
                     /locus_tag="IXT20_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HAQ4860742.1"
                     /translation="MTKNIFDSIANHYDSPDRQALAKIIREELTTYLPRDSHQKVLLD
                     YGGGTGLVSLPLAERFKELIIADASETMLKMAEEKIQAADLKNVRTIHADASVEFPAV
                     QANLILLSLVLLHIPDTENILTKLYEILAPGGQLIIVDFDKNEQISHPKVHNGFSQEE
                     LNNRLEKTGFVSATSHTFHRGEKLFMNKNASLFLSISQKA"
     gene            765..1234
                     /locus_tag="IXT20_002315"
                     /pseudo
     CDS             765..1234
                     /locus_tag="IXT20_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325408.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            1340..1924
                     /locus_tag="IXT20_002316"
     CDS             1340..1924
                     /locus_tag="IXT20_002316"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HAQ4860743.1"
                     /translation="MAQLFFKYGAMNSGKTIEILKVAHNYEEQNKPVVLMTSGIDTRD
                     GLGVVSSRIGLKRNAMPIFEETDVFDVIQKMESKPYCVLIDEAQFLKKKHVIAFTRIV
                     DELDIPVMAFGLKNDFRNELFEGSKYLLLYADKIEEMKTICWFCHKKAIMNLHYIDGN
                     PVYEGDQVQIGGNEAYYPVCRKHYFHPSMITEGE"
     gene            1927..3000
                     /gene="prfA"
                     /locus_tag="IXT20_002317"
     CDS             1927..3000
                     /gene="prfA"
                     /locus_tag="IXT20_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HAQ4860744.1"
                     /translation="MYDQLQAIEDRYEELGELLSDPEVISDTKRFMQLSKEEANTRET
                     VEVYRRYKEVVQGITDTEELLGEKLDDEMAELAKEELSELKKEKEVLEEKIKILLLPK
                     DPNDDKNIIMEIRGAAGGDEAALFAGDLFNMYQKYAEAQGWKTEVMEANVTGIGGYKE
                     VIMMITGDNVYSKLKYESGAHRVQRVPSTESQGRIHTSTATVVVMPEAEEVEIDIEDK
                     DIRTDIYHASGAGGQHVNKTASAVRLTHLPTGIVVAMQDERSQIKNREKAMKILRARV
                     YDKIQQEAQSEYDANRKSAVGTGDRSERIRTYNFPQNRVTDHRIGLTIQKLDQILAGK
                     LDEIVDALIMYDQTSKLEAMQNG"
     gene            2993..3832
                     /gene="prmC"
                     /locus_tag="IXT20_002318"
     CDS             2993..3832
                     /gene="prmC"
                     /locus_tag="IXT20_002318"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HAQ4860745.1"
                     /translation="MGKMTYREVLSRASSFLEDHGKEAYSIQFLFLERKQWKKLDWLL
                     HMNEEISEEEQRLIETDLRLLLADHPPQYLLGYADFYDHRLKVTEATLIPRPETEELV
                     EWCLDETPGVPLEVIDIGTGTGAIAISLKAARKNWHISAVDLSEEALEVAKENAQKEG
                     TKISFYHGDTLGPVMDQQFDVIISNPPYISRNEWELMDESVRSFEPKMALFAENDGLA
                     IYEKIAVEAPSVLKSNGKIFLEIGFRQGEAVKKIFQQAFPDKKVAIKKDLFGNERMIQ
                     VYG"
     gene            3825..4004
                     /locus_tag="IXT20_002319"
     CDS             3825..4004
                     /locus_tag="IXT20_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4860746.1"
                     /translation="MVEVVDEKFFICFFIIKKHLFDKDVNDFLVYQQVYPLLTNRLST
                     NSGKLEDKLRQNKNV"
     gene            4026..5051
                     /locus_tag="IXT20_002320"
     CDS             4026..5051
                     /locus_tag="IXT20_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HAQ4860747.1"
                     /translation="METKRYDETELQEAAEALKEGELVAFPTETVYGLGANALLPNAV
                     KKVFSVKGRPQDNPLIVHVASFEQVKEYVDNFHPAAEKIVKNFWPGPLTLIFKIKKDT
                     LPSVVTGGLSTAAFRIPDNKKTLEVIELSGVPLVGPSANTSGKPSPTTADHVYHDLQG
                     KITGIIDDGATRIGVESTVLDLSDPTAMPMILRPGAVTKEQIEAVIESPVAIDQHLVK
                     ENETPKAPGMKYKHYSPDTRVLMVRDGDWSTAVQWAKNKKIRAGVIASPEIADQVRTD
                     TAAVYMYNDNSVEAAAKGLFAGLRGLDEPTLGLDLIFVQVYPETGLGNAYMNRLKKAA
                     GQNYFEK"
     gene            5226..6470
                     /locus_tag="IXT20_002321"
     CDS             5226..6470
                     /locus_tag="IXT20_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HAQ4860748.1"
                     /translation="MVDYKTFDPDLWAAIAKEEERQEHNLELIASENFVSEAVMAAQG
                     SILTNKYAEGYPGHRYYGGCEFVDIVENLAIDRAKELFGAKFANVQPHSGSQANTAAY
                     LALVEPGDTILGMDLSAGGHLTHGSPVNFSGKTYHFVAYGVDPTTEVIDYNVVRILAR
                     KHQPKLIVAGASAYGRTIDFAKFREIADEVGAKLMVDMAHIAGLVAAGLHPNPVPYAD
                     ITTTTTHKTLRGPRGGMILTNDEALAKKINSAVFPGIQGGPLEHVIAGKAVAFKEALD
                     PAFKEYSEQIIANAKAMVKVFNQAIGTRVISGATDNHLMLIDVRELGINGKEAESILD
                     SVNITVNKNSIPFETLSPFKTSGIRIGTPAITTRGFKEEDAVKVAELVVKALQAKDDN
                     AQLDEVKTGVRELTEKFPLHKK"
     gene            6623..7570
                     /locus_tag="IXT20_002322"
     CDS             6623..7570
                     /locus_tag="IXT20_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAQ4860749.1"
                     /translation="MTDRISLEKEAVAFSDANAPHPRIYELAPEEGRVLLEEVQESPV
                     NKYSVDIEDLMVDTGEWGKLNIRLVRPEGNMDKLPVIFYIHGAGWVFGSAQTHDKLIR
                     ELAVRTNSVVVFPEYSRSPEAKYPTAIEQNYAVLQQLNKLAKSKNLDLENLIVAGDSV
                     GGNMATVMTILTKERKGLPISKQLLYYPVTNADFDTDSYHEFGENYFLTREGMMWFWD
                     QYTTNEEERAEITASPLRANINELNDLPSAMILTGEADVLRDEGEAYARKLRDAGVPV
                     TQVRFQGIIHDFVMVNAMDQTKATRAAMALSTSWIKKKE"
     gene            7704..8333
                     /gene="upp"
                     /locus_tag="IXT20_002323"
     CDS             7704..8333
                     /gene="upp"
                     /locus_tag="IXT20_002323"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013774406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HAQ4860750.1"
                     /translation="MGKFQVIDHPLIQHKLTIIRDKNCGTKVFREVVDEIAMLMAYEV
                     SRDMPLEDIVIETPITETTQKTLSGKKVAIIPILRAGIGMVDGILELIPAAKVGHIGL
                     YRDEETLEPHEYFVKLPEDIDARQLFVVDPMLATGGSAIMAIDALKERGASNIKFVCL
                     VAAPEGVKALQEAHPDIDIYTASLDEKLNEHGYIVPGLGDAGDRLFGTK"
     gene            complement(8472..8714)
                     /locus_tag="IXT20_002324"
     CDS             complement(8472..8714)
                     /locus_tag="IXT20_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4860751.1"
                     /translation="MKMATFGKFDTTIVPSEIGEKFKINTHVMFTIHGKSQTGTISKQ
                     LKNSAVVEIDETAENANLMTQSKGVVIVNYKELKKI"
     gene            8835..8907
                     /locus_tag="IXT20_002325"
     tRNA            8835..8907
                     /locus_tag="IXT20_002325"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8868..8870,aa:Thr,seq:cgt)
     gene            complement(9026..9664)
                     /locus_tag="IXT20_002326"
     CDS             complement(9026..9664)
                     /locus_tag="IXT20_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HAQ4860752.1"
                     /translation="MNNILGKKIELKKGILGTIVVVLGLLGLLFAPSIISIFSLTITG
                     FIAFTLVFGKKDTKKMFSKPVAPVKNIAKYFFINVVISAAVSVFLQQILKWGLTGNPI
                     NEAFSPLLFIILPIMILGEELFSVYFLAIFSSKFSIPVASFLSAIIFGFIHYSTYDNG
                     NILHTVAHILLIQGVARLLFNQAAIKSNSIITSWAVHVIFDFTAILLVVLFS"
     gene            complement(9787..10419)
                     /locus_tag="IXT20_002327"
     CDS             complement(9787..10419)
                     /locus_tag="IXT20_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HAQ4860753.1"
                     /translation="MIFPKVNEASLNKGSFFHIYENEDFELYNILSQKEVIENQIICH
                     PIIYKSIFKYAFFASNDFQRMEITDCIFENCDFSGCILTKSMLHRVKFVNCKLTGTKL
                     MESYIGNTLFENCKMDYVNLSGSNIKESTFEHSVLSSADFVDCSLTKIMFSTNDLENA
                     SFFDTDLKNIDLSSNSFEMIEVRSEHLKNCTLNQLQALGFARKFLQIKVV"
     gene            10770..11069
                     /locus_tag="IXT20_002328"
     CDS             10770..11069
                     /locus_tag="IXT20_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HAQ4860754.1"
                     /translation="MSKSEEIENMVLKLYDLVLNPEIKEKERILLIDAKTGLEKGQYY
                     PKVINNLERSLRPLAIRGELSKPVSPFYMEISTIGKFEKELGRGMASAPITFGHL"
     gene            11564..>11903
                     /locus_tag="IXT20_002329"
     CDS             11564..>11903
                     /locus_tag="IXT20_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HAQ4860755.1"
                     /translation="MKKCRNIILSMLFIITNITSLIPVHVYADAGRDISSNVTSLTVD
                     PTNITDGGNIKVKFSFDEKKQNIQPGDYLWINWPSEGNIRGEGFQKEIPLMIENKNVG
                     TLTVRKDSAQV"
BASE COUNT         4133 a         1936 c         2419 g         3415 t
ORIGIN      
        1 tttccgaaaa ttccttctta tttcttggag ttgctcactt tttttgcaat ctcattttaa
       61 gctttttggc tgatggataa gaaaagcgaa gcgtttttgt tcataaatag cttttcccca
      121 cgatggaagg tatgactagt agcagagaca aagcccgttt tttccaatcg attattcaat
      181 tcttcttggc taaagccatt atggactttc ggatgactaa tttgttcatt cttgtcaaag
      241 tctacgatga tcaattgacc gccaggagct aaaatttcat aaagttttgt aagaatattc
      301 tccgtatcag gaatgtgtaa aagtacaagt gatagaagga tgagattagc ttggacagca
      361 ggaaattcga cagaagcatc agcgtggatc gttcggacat ttttcagatc tgctgcttgg
      421 atcttttctt ctgccatttt cagcatagtt tctgaagcat cggcaatgat cagttctttg
      481 aatctttccg caagcggtaa actaacaagc ccagttccgc ctccgtaatc cagcaggact
      541 ttttggtggc tgtcgcgcgg tagataagta gttagttctt cgcgaataat cttggctaat
      601 gcttgacggt caggggaatc ataatggtta gcgatggaat caaaaatatt ttttgtcatc
      661 aaatagctcc ttacgtaaaa ctttgaaatt ttaacttatt atagcacgtg aaaagaaaga
      721 aagcatgcta tactcaattc aaaaagggag aatctagaaa gaatatgccg atatcgtttg
      781 taaaagatag agaggaaaaa ggaaagtgtg tccgagaaat attactagac ttgcctgagt
      841 ggtttgggct tcctgaatca acggaaaaat atatcgagga atcaagtaaa ctacctttat
      901 ggtgtgaaaa aagaaaagaa gaatatttag gctttatcac tctttcacag acaagtgaag
      961 atactgccga aatctattct attgtatggg aataaaaaaa gactatcatc accaaggatt
     1021 aggaacaaaa ctttttaaag aagcagaagg ttacgcagca aaacattaca aatatttgca
     1081 agtgaagact gtagacgagg ggcactactc tatatatgat caaactattt gtttctacga
     1141 atcattagga ttttcaaggt tggaagtttt tccaaattta tgggatgaat ggaacccatg
     1201 tttagttctt gtcaaaaaat tggaacaaaa gtgagcaata gatttaatac taatctgtgc
     1261 ttgatgaaaa ttctcactgt aaaaaatgct gaaaatcaag tataatacca taggaacaaa
     1321 ctggaaggtg aaaaagagca tggcacaact attttttaag tatggcgcaa tgaatagcgg
     1381 caagacgatc gagatcctaa aagtcgcaca taattatgaa gaacaaaata agccagttgt
     1441 cttaatgaca agcggtattg atacaagaga tggtttaggc gttgtttcaa gccgaatcgg
     1501 actgaaacgt aatgcgatgc cgatatttga agaaacagac gtatttgacg taattcagaa
     1561 aatggaaagc aaaccctatt gtgtgctgat cgatgaagcg caattcttaa aaaagaaaca
     1621 tgtcattgct tttacgagaa ttgtggacga attggatatt ccggttatgg catttggttt
     1681 gaaaaatgat tttcgaaacg agttatttga aggatcaaaa tatttattgc tttatgcaga
     1741 caaaatcgaa gaaatgaaaa caatctgttg gttctgccac aaaaaagcaa tcatgaattt
     1801 acattatatt gatggaaacc ctgtttatga aggcgatcaa gtgcaaattg gcggaaatga
     1861 agcttattac cccgtttgtc gaaaacatta ctttcaccca tcaatgatta cagaaggaga
     1921 ataagcatgt acgatcaatt acaagcaatt gaagaccgtt atgaagagct tggagaactt
     1981 ttaagtgacc cagaagtgat ttctgataca aagcgtttta tgcagctttc aaaagaagaa
     2041 gcaaacacgc gcgaaacagt cgaagtttat cgtcgttata aagaagtagt acaaggaatc
     2101 accgatacag aagaactgct tggtgaaaaa ctggatgatg aaatggctga actagcaaaa
     2161 gaagaattat ctgaattaaa aaaagaaaaa gaagtcttag aagaaaaaat caagatcttg
     2221 cttttaccaa aagatccaaa tgacgataaa aatatcatca tggaaatccg tggagcagca
     2281 ggcggagacg aagcagcatt attcgctggc gatcttttca atatgtacca aaagtatgct
     2341 gaagcacaag gctggaaaac agaagtgatg gaagctaacg ttaccggaat cggtggatat
     2401 aaagaagtga tcatgatgat caccggagac aacgtatatt cgaaattaaa atatgaaagt
     2461 ggtgcgcacc gcgtccagcg tgttccttca actgaatcgc aagggcgtat ccatacttcc
     2521 accgcaacag ttgtcgtgat gccagaagct gaagaagtag agatcgatat tgaagacaaa
     2581 gatatccgta cagatatcta tcatgcttca ggtgctggcg gacagcacgt caataaaaca
     2641 gcatctgctg tacgtttgac tcacttacca acagggatcg ttgttgctat gcaagatgaa
     2701 cgttctcaaa tcaagaaccg tgaaaaagca atgaaaatct tgcgtgctcg tgtgtacgat
     2761 aagatccagc aagaagcaca aagcgagtat gatgccaatc gaaaatcagc agtcggaact
     2821 ggggatcgtt cagaacggat ccggacgtat aatttcccgc aaaatcgtgt gactgatcat
     2881 cgtatcggat taactatcca aaaattagat cagattttag cagggaaatt agatgagatc
     2941 gttgatgcat tgatcatgta tgatcaaaca tcaaaactgg aagctatgca aaatgggtaa
     3001 aatgacctat cgagaagtcc tgtcacgggc ttcttctttt ttagaggatc atgggaaaga
     3061 agcatatagt atccaatttt tatttttgga acgaaaacaa tggaaaaaac ttgattggct
     3121 ccttcatatg aacgaagaga tttcagaaga agaacagcgg ttgatcgaaa ccgatctgcg
     3181 acttctgctt gcagatcatc cacctcagta tttgctagga tatgcagatt tttatgatca
     3241 tcggttaaag gtaactgaag caacactgat ccctcgacca gaaacagagg agttagtcga
     3301 atggtgttta gacgagacgc ctggtgtacc gttagaagtg atagatattg ggacagggac
     3361 tggagcaatt gctattagtt tgaaagcagc aagaaaaaat tggcatatat ctgctgtgga
     3421 tctttcagaa gaagcattag aagtagcgaa agaaaatgct cagaaagaag gaacgaaaat
     3481 cagtttttat catggagata cattaggacc agtaatggat cagcaatttg atgtgattat
     3541 ttctaatccg ccgtacatca gtcggaatga atgggaactg atggatgaaa gtgtccggtc
     3601 attcgaacct aaaatggccc tttttgctga gaacgatgga ttagctattt atgaaaaaat
     3661 agcagttgaa gcaccaagcg tactaaaatc aaatggaaag atttttttag aaatcggatt
     3721 tcgccaaggt gaagcagtaa aaaaaatatt tcagcaagca tttccagata aaaaagtggc
     3781 gataaaaaag gatctttttg gcaatgaacg aatgatccaa gtttatggtt gaagttgtgg
     3841 atgaaaaatt ttttatttgt ttttttatta taaaaaaaca cttatttgac aaggatgtaa
     3901 acgacttttt ggtttatcaa caagtttatc cactattgac aaatagatta tccacaaaca
     3961 gtggaaaact tgaggataaa ctcagacaaa ataagaatgt atgaaaaagg ggttgtggat
     4021 aaactgtgga aacgaaacga tatgatgaaa cagaattgca agaggcagcc gaagcactga
     4081 aagaaggaga acttgttgct tttcctaccg aaacagtata cggattgggc gccaacgctc
     4141 ttttaccaaa cgctgtcaag aaagtgtttt ctgtaaaagg gagaccgcaa gataatccat
     4201 taatcgtcca tgtcgcctct tttgaacaag taaaagagta tgtggacaac tttcatccgg
     4261 cagcagagaa gattgtgaag aacttctggc ctggaccatt aactttgata tttaagatca
     4321 aaaaagacac tttgccctca gttgtgacgg gaggattatc cacagctgca tttcgtatac
     4381 cggataacaa aaaaacacta gaagtaatcg aactttccgg tgttccgctt gtcggtccaa
     4441 gtgcaaatac gtcgggaaaa ccaagtccaa cgactgcaga tcatgtatat catgatctac
     4501 aaggaaaaat taccgggatt attgatgatg gagctacgcg aattggtgta gaatccactg
     4561 ttcttgattt aagtgatcca acagccatgc ctatgatttt acgaccaggt gcagtaacga
     4621 aagaacaaat cgaagcagtc atcgaatctc ctgttgctat tgatcagcat cttgtaaaag
     4681 aaaatgaaac accaaaagcc cctgggatga aatacaaaca ctactcaccc gatacacgtg
     4741 ttctgatggt cagagatggc gattggtcaa ctgcagttca atgggcaaaa aataagaaga
     4801 tccgtgcagg tgtcatagca agccccgaaa ttgcggatca agtgcggaca gatactgcag
     4861 ctgtttatat gtataacgac aactcagtag aagcggcggc taaaggattg tttgcaggtc
     4921 taagaggatt agatgaacca acacttggat tagatttgat ttttgtacaa gtgtatccag
     4981 aaactggatt ggggaatgct tacatgaatc gcttgaaaaa agcagcagga caaaattatt
     5041 ttgaaaaatg atcaattggt tattgtcgca ttcttttagt atgatacaat gctatcaagt
     5101 aaatcttcta tcagtgaacg aaaaactaag cgtaactaat cgtaatagaa gagatacgaa
     5161 tatttggcaa tttgtttttg atgggaaaaa taaaattgta ggaaaataaa aaggagctgt
     5221 tgtttgtggt agattacaaa acgtttgacc ctgatctttg ggctgcgatc gccaaagaag
     5281 aagaaagaca agaacataat ttggagctga ttgcctctga aaactttgtc tccgaagcag
     5341 tgatggctgc acaaggaagt atcttgacaa ataaatacgc agaaggttat ccagggcatc
     5401 gctattatgg tgggtgtgaa tttgtcgata tcgttgaaaa tttagcgatt gaccgtgcaa
     5461 aagaattatt cggagcgaaa tttgcgaacg tccagcctca ctccggctct caagccaaca
     5521 cggctgctta tctcgctttg gtagaaccag gggacacgat cttaggaatg gatctttccg
     5581 caggcggaca tctaacacat ggatcaccag tcaacttcag tggcaaaacc tatcactttg
     5641 ttgcttacgg tgttgatccg acaacagaag ttattgatta taatgtggta cgtattttag
     5701 ctagaaaaca tcaaccaaaa ttgatcgtag caggtgccag tgcttacgga cgaacgatcg
     5761 actttgcgaa attcagagaa atcgcagatg aagtaggcgc aaaactgatg gtagatatgg
     5821 cacatatcgc aggattagta gcagcaggac ttcatcctaa tccagttcct tatgcagata
     5881 tcacaacgac taccactcat aaaacattgc gtggccctcg aggcggcatg attttgacaa
     5941 acgatgaagc attagcaaaa aaaataaata gtgcggtatt tcctggtatt caaggcggcc
     6001 ctctagagca tgtgattgcg ggtaaagcag tagcattcaa agaagcatta gatcctgcat
     6061 ttaaagaata tagtgaacaa atcatcgcca atgcaaaagc aatggtcaaa gttttcaatc
     6121 aagcaattgg aaccagagtc atttcagggg cgacagataa tcatctgatg ttgatcgatg
     6181 ttcgcgagtt aggtataaat ggaaaagaag cagagagcat tttggacagt gtgaatatca
     6241 ctgtaaataa aaactcgatt ccttttgaaa cactaagtcc attcaaaaca agcggtattc
     6301 gaatcggcac gccagcaatc actactcgcg ggttcaaaga agaagatgct gtaaaagtgg
     6361 ctgaactagt tgtcaaagca ttacaagcaa aagatgataa cgctcaacta gacgaagtca
     6421 aaacaggtgt acgcgaatta actgaaaaat ttccgttgca taaaaaataa aaatttaaat
     6481 tccattagta aacagaaaag ttcaatgttg ggcttttctg tttatttttt gggaaaacgg
     6541 attctttgac aatatcggtg atgctctcta gtatatgagg tgtaatttta gtcttcacac
     6601 taaatctcag ggaggaaaag tcatgactga tcgtatctcg ttagaaaagg aagcagtagc
     6661 attcagtgat gcaaatgcgc ctcatccgcg tatctatgaa ctagcaccgg aagaaggaag
     6721 agttttacta gaagaagtac aagaatcacc tgtgaacaaa tattccgtcg atattgaaga
     6781 cttgatggtg gatactggcg aatggggaaa actcaatatt cgtctcgtac gaccagaagg
     6841 gaatatggat aaattaccag ttatttttta tattcatgga gcaggctggg tttttggcag
     6901 tgctcaaacc catgataaat tgatcagaga acttgcggtt cgtacgaatt ccgtcgttgt
     6961 ctttccagaa tatagtcgat caccagaagc aaaatatcca acagcaattg aacaaaatta
     7021 tgctgtgttg caacagctga ataaattagc caagtcaaaa aatctggatc tcgagaatct
     7081 aatcgttgca ggagattcag taggtggtaa catggctaca gtcatgacga tcttaacaaa
     7141 agaacgaaag ggcctcccaa tatccaagca attgctgtat tatccagtca caaacgctga
     7201 ttttgataca gattcctatc atgaattcgg agaaaactat tttttgacaa gagaaggaat
     7261 gatgtggttt tgggaccaat atacgacgaa tgaagaagag cgtgctgaaa tcacagcttc
     7321 gcctttgaga gcaaatataa atgaactaaa tgatcttcct tcagcaatga ttttaacggg
     7381 agaagcggat gtattaagag atgaaggtga ggcttatgcg cgcaaacttc gtgatgcagg
     7441 ggttcctgtt acacaagtac gcttccaagg aatcatccat gattttgtca tggtaaatgc
     7501 tatggatcag acaaaggcaa cgcgtgcagc aatggcatta tctacttctt ggatcaagaa
     7561 aaaagaataa gctttgcttg tccgcaggaa aacttctttt caaaaacgga ttttcttagg
     7621 aaaaatggcg agtttgacta gtcaatcact tgattttgtt ttacaattaa aaagaaacga
     7681 cagaacaaag gagagcttaa aaaatgggca aatttcaagt gatcgatcat ccattgattc
     7741 aacacaaact aactattatt cgggacaaaa actgcggaac aaaggttttt cgtgaagtag
     7801 tcgatgaaat cgctatgttg atggcatatg aagtatcacg ggatatgcca ttggaagaca
     7861 tcgtgatcga aacaccgatc actgaaacaa cacaaaaaac gttgtctggt aaaaaagtag
     7921 cgattattcc aatcttgcgc gcaggtattg gaatggttga cggtatcttg gaattgattc
     7981 cagctgctaa agtgggacat atcggtcttt atcgagatga agaaacattg gaaccacatg
     8041 aatatttcgt caaacttcca gaagacattg atgcacgtca gttgtttgta gtagatccaa
     8101 tgttggcaac aggcggatca gcaatcatgg caatcgatgc attgaaagaa cgtggcgcgt
     8161 cgaacattaa atttgtttgt ttggtagcag cacctgaggg agtaaaagct ttgcaagaag
     8221 cgcatcctga tattgacatc tatacagctt ctctagatga aaaattgaat gaacatggtt
     8281 atatcgttcc tggactaggg gatgcgggtg accgtttgtt tggtacaaaa taagtaaata
     8341 cgaaaaaggg tgtgacacaa cttttgtcac atcctttttt ggtcattttt aacgtttgat
     8401 gcagaacagc tttttcacag ctttcgtttg catataaaaa aaccatccca aaaatgcaga
     8461 gatggtcaaa ttcaaatctt ttttagttct ttgtagttaa cgattactac acccttactt
     8521 tgcgtcatca agttcgcatt ttctgcagtt tcatcaatct caacaacagc ggaatttttc
     8581 aattgctttg aaatcgttcc agtttgcgac ttgccgtgaa ttgtgaacat tacgtgagta
     8641 ttgatcttga atttttcacc aatttccgat ggtacgatag ttgtatcaaa ttttccaaaa
     8701 gttgccattt tcataaaacc tgcctttcac atcaattata ccacgtttta tgaaaaatat
     8761 catgaaatta aactaatttt ccatcggact ctttacaatc agccaaatag agtatataat
     8821 ttcttttgga taaggccagc ttagctcagt tggtagagca acgcactcgt aacgcgtagg
     8881 tcgtaggttc gatccctata gctggcatta aaagagaaga cagatatatc aaggattttt
     8941 aatccattga tacatctgtc tttttgttta aaggaaataa agtatgcttt cttagcaata
     9001 gagcatgtat tatttctata aaaatttagg agaataaaac gactagcaat atagctgtga
     9061 aatcaaatat cacatgaact gcccaacttg taatgatcga gttgctttta atggcagctt
     9121 ggttgaataa caatcttgcc accccttgta tcaaaaggat gtgtgctaca gtgtgtaaaa
     9181 tatttccatt atcataagta ctgtaatgga taaatccaaa gatgattgct gacaagaaac
     9241 tagcgacagg aatgctgaac ttactagaaa aaatagcgag aaaatacact gagaacaatt
     9301 cttctccaag tatcatgata ggcaaaataa taaataataa cgggctgaaa gcctcattta
     9361 ttggattgcc tgttaaaccc catttcaaaa tttgttgcaa aaataccgag actgcggcac
     9421 tgattactac attgataaaa aaatatttgg ctatattttt gacaggagcg acaggtttcg
     9481 agaacatttt tttcgtatct tttttaccaa aaacaagtgt aaatgctata aatccagtga
     9541 tagttaaaga aaaaatgcta ataatggatg gcgcaaatag caaccctaac aaaccgagta
     9601 caacgacaat agtgcccaat atcccttttt taagttctat tttttttcct aaaatgttat
     9661 tcatgataat aactcctact attcagtttt tgaagataga gtcagtatac atgaagagtt
     9721 aaaataggca atgttttttt atggatattt gtattgctaa aaatttatct tatatattca
     9781 ttttgattat actactttta tttgtaaaaa ttttctagca aaaccaagtg cttgcaactg
     9841 attaagcgta caatttttta gatgttccga gcgtacttca atcatttcaa aggagttgct
     9901 acttaaatcg atatttttta aatcagtatc aaaaaagcta gcattttcta aatcattagt
     9961 agaaaacatt attttagtga gggaacagtc tacaaaatct gcgcttgata agacagagtg
    10021 ctcaaaggta ctttctttta tgtttgaacc agataaattt acatagtcca ttttacaatt
    10081 ctcgaataac gtgttcccaa tataactttc catcaattta gtacctgtta atttacaatt
    10141 gacaaatttc acgcgatgca acatgctttt tgtcaatata cagcctgaaa aatcacagtt
    10201 ttcgaatata cagtcggtaa tctccattct ttgaaaatca ttggaagcaa aaaatgcata
    10261 cttgaaaata gatttgtaaa taattggatg gcagattatt tggttttcga tgacctcttt
    10321 ttgtgaaagg atattataca gttcaaaatc ttcgttttca tagatatgaa aaaaggatcc
    10381 tttattcaag cttgcttcat taactttagg aaaaatcata tttaattacc tccattttca
    10441 taaagtcata ttaacaaaag ctaaaccaac ttacattagc agaaaatgag ataggttttc
    10501 gcctttttcg ataacgatag tgtcgctatc tcttgaaacg ttcttagaat tgccctgaga
    10561 gacacaaatt aggcgtaatt ctaataaaaa tcttagaata aaaattttta ctgatttaat
    10621 ttagaatgtt tttaaaataa aaatcgttgc cataacagtg tattgaaagg ttttaacaat
    10681 ccagtaatga tttaaaaaaa tctagatgta gcgtatactt tgtttataga atgtagtggg
    10741 aagaaatata tttttgggag gaaatcgtga tgtctaaaag tgaagaaata gaaaacatgg
    10801 tactaaaatt atatgatctc gttttaaatc cagagatcaa agaaaaggaa cgtatacttt
    10861 tgatagacgc aaagacaggc ttagaaaaag gtcagtatta tcctaaagtc ataaataatt
    10921 tggaacgtag tttacgtccg ttagcaatca ggggagaatt gtctaaacca gtatcaccgt
    10981 tttatatgga gatatcaaca ataggaaaat ttgaaaaaga attaggtcga ggaatggctt
    11041 cggcaccgat tacttttggg catttataaa aacgagttat tcatctcaat gaattaaaaa
    11101 gtatagtcaa tctcataatg caggctaggt tcatttattt gaacagagtc tgtatttttt
    11161 tattctaaat aaagaaacag atgtttcttt atattgtatt ttatccttta attaacacaa
    11221 aaacagctag tataaactaa taatgttaat taaaagtgta ttttatgaaa ctatcgttat
    11281 ttattaatgt tgcatttttg taagttaaaa attggaaata tagattgaaa aaatgaaagc
    11341 aataagtaaa ataaaatgaa tatatgcgaa aaaataattt acattaggaa aaaccttgta
    11401 aaaacagttt tttggcatat ttatttttta taaatatttg taaaataact atcaattttt
    11461 tagttaaaag attggaaatg agtgggggag gaattgaaga aaggattttt gagagatgat
    11521 atagtaggtt ttatttttag ttaaaatagg ggagatagaa aaaatgaaaa agtgtagaaa
    11581 tattatttta tcaatgttat ttattattac taatataaca tcactgattc ctgttcatgt
    11641 ctatgcggat gcaggcagag atatcagcag taatgtcact tcgttgaccg tggacccaac
    11701 gaatattaca gatggaggaa acataaaagt aaaattctcg tttgacgaaa aaaaacaaaa
    11761 tatccaacca ggtgattatc tttggatcaa ttggccaagt gaaggaaata ttcgcggtga
    11821 aggattccaa aaagaaatcc cattaatgat tgaaaataaa aatgttggaa ctttaacggt
    11881 cagaaaagat tctgcgcagg ttg
//
LOCUS       DACGLE010000080        11570 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0534
            SAMEA3304449-rid9560453.denovo.084, whole genome shotgun sequence.
ACCESSION   DACGLE010000080 DACGLE010000000
VERSION     DACGLE010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304449
            Sequence Read Archive: ERR985064
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11570)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11570)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702225.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702225.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 20:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,045
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 27 of 67
            Pseudo Genes (incomplete)         :: 25 of 67
            Pseudo Genes (internal stop)      :: 19 of 67
            Pseudo Genes (multiple problems)  :: 4 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11570
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304449-rid9560453.denovo.084"
                     /strain="VREN0534"
                     /db_xref="taxon:1352"
     gene            complement(203..1654)
                     /gene="cls"
                     /locus_tag="IXT20_002330"
     CDS             complement(203..1654)
                     /gene="cls"
                     /locus_tag="IXT20_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HAQ4860756.1"
                     /translation="MVSSIITALYLLNALIALVAILIKPRDVAAIWAWLLVLFALPGV
                     GFVLYLFFGRGLTDKKKFYLRQSDLKELENFQSFRGDTIEHYDPDMGDKDKQQFVDFF
                     SSLNRMPLTRMNSVTLLTDGQEKLDSLLQDLKKAKHSIHIEYYAFVTDNIGQQVLHVL
                     EEKAAEGVEVRILYDAFGSHGTKAKDFNRLIKNGGHVHTFVTSQRALLRFRLNYHDHR
                     KIVVIDGKISYTGGFNIANQYVNTTKKFGYWRDTHIRIFGAASSLLQLRFLTDWNVSV
                     PEEKKVGYHLNYFFKKADRDESKLADTSIQLVSSGPNNEREQIKLSFIKLITSAKKRV
                     WIQTPYLVPDESVIAALKIATASGVDVKIMIPNKPDHPFIYRATQYYARQLIKENVQI
                     LVYENGFLHAKTLIMDDEICMVGSANQDIRSYRLNFETSAVIYDPEFLEELATQFKED
                     ETHCSSMTTETVKEMSNWLLFKQQISRLFSPIL"
     gene            1995..4661
                     /locus_tag="IXT20_002331"
     CDS             1995..4661
                     /locus_tag="IXT20_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HAQ4860757.1"
                     /translation="MENKEQAQQAVYKETIDSLMQQTQSSTDGLSTTQAKEKLERDGL
                     NQLKEKPKKPTWKLFLETFKDAMVIVLLIVALIQMVMGDYIEFLVIFAVLMLNSIISV
                     VQTKKAEGSLEALKNLSAPDARVIRDGKEKTIPANELVVGDIVLLDAGDYVPADGRLL
                     DAGSLKVDEGMLTGESTSAEKEVTDITSTVPIGDRTNMVFSGTIVTYGRGKFLVTSTG
                     NNTEIGQVAGLLESTTEQVTPLQRGLDKFSKKLSLAILALSLLILGIQLFRIYLGEGT
                     GDMTADIVSAVMFAVAVAVAAIPEALQSIVTIVLSLGTNKMAKRHAIIRKLPAVETLG
                     STSIICTDKTGTLTQNKMTVVDYFLGNGDTGDFTNDPSKWTADERRLIEISVLANDAS
                     ISEDGTKLGDPTEVAFIDFSEKLNQPYQEIRNNYPRQAELPFDSDRKLMSTGHTIDNK
                     NYLFVKGGPDIVFQRSKKVLLNGEVVPMTDELMKKFQEQNEAFSTRALRVLAFAYRPL
                     DNFDLSFEDEDDLILVGLLAMIDPPRAEVTQAVTEARSAGIKTIMITGDHKTTAVAIA
                     KQIGIFEEGNLALTGTELDELTEDQLNEVLEKVTVYARVSPENKIRIVRAWQNKGHIS
                     AMTGDGVNDAPALKQADIGIAMGTGTDVAKDAAAMVLTDDNFASIVSAVEVGRNVFDN
                     IKKAISYLFAGNLGAIIAIIFALVADWSNPFTALQLLFINLVNDSLPAIALGTEKAEP
                     ATMNRPPRDPNSGIFTDKTLISVTYRGVLIGIAVIFAQWLGMQHSPEFGVAMSFSTLI
                     WCRTLQTFPARSNTQTAIQAGFFSNRSVLLAVVVCSALYCLTMIPGLKEVFSIPSAFG
                     LTEVFTCMGIALVTIVLMEITKLIIVRVQGNK"
     gene            5025..5777
                     /locus_tag="IXT20_002332"
     CDS             5025..5777
                     /locus_tag="IXT20_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAQ4860758.1"
                     /translation="MNEKEWDEFAEEYYEIQQESQVTIAEDVKNYLKTEKVLPAETAA
                     DVAGGSGRFLSLAKEINRYDLIDFSSGMLENAKKEAEKNELPNVSFIKQSLEEFIQSN
                     ETYEMVFTAANPALVHLIQLEKLMKKSSKWCVIIRVVESIDDVFYPIEKQLGMIEEDP
                     NTSPVVMDQFETYLIDEGFKVKKKDFTYFTQESITEELMKLYYDAYAKDPVVTRYIRK
                     LFSEKREILSTTKLTYRLLLWSADKKRTFHDS"
     gene            6230..7618
                     /gene="ltrA"
                     /locus_tag="IXT20_002333"
     CDS             6230..7618
                     /gene="ltrA"
                     /locus_tag="IXT20_002333"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HAQ4860759.1"
                     /translation="MRRPQKTRKLGYHQRASVEHEGYDEVRSTSCGEQRKQDGVENLF
                     GRILDRNNLNQAYLQVVRNKGAAGADGMTVDQLLPYLKEHREELLSNLRQGNYKPQPV
                     LRVEIPKPNGGVRKLGIPTVIDRMLQQAINQVLQPLFEPEFSDNSYGFRPNRSAHDAV
                     KKAKSYYEQGYRYVVDIDMKAYFDTVNHDKLMYFIERKVKDKQVLKLIRQYLMSGVME
                     NGLITTTEEGTPQGGNLSPLLSNIYLNEFDQVLENLGHKFVRYADDCNIYVRSKRAGQ
                     RVMKKSIQFLEEQLKLTVNREKSAVGSPLKRKFLGFCILPTKKGIKIRPHAKAKQRVK
                     SKLKQLTKRNRGRSIQLILKEIKQLMTGWINYYGIGEMKEFMRELNGWLKRRIRQYIW
                     KQWKNPRTKRKNLIRLGIDKAKAYEWSNTRKGIWSVSKSHILHRSLTDKELARQGYED
                     ISLKYQFVHSTY"
     gene            complement(7698..8852)
                     /locus_tag="IXT20_002334"
     CDS             complement(7698..8852)
                     /locus_tag="IXT20_002334"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HAQ4860760.1"
                     /translation="MKIGIDRLSFFIPNLYLDMTELAESRGDDPAKYHIGIGQDQMAV
                     NRANEDIITLGANAASKIVTEKDRELIDMVIVGTESGIDHSKASAVIIHHLLKIQSFA
                     RSFEVKEACYGGTAALHMAKEYVKNHPERKVLVIASDIARYGLASGGEVTQGVGAVAM
                     MITQNPRILSIEDDSVFLTEDIYDFWRPDYSEFPVVDGPLSNSTYIESFQKVWNRHKE
                     LSGRGLEDYQAIAFHIPYTKMGKKALQSVLDQTDEENQERLMARYEESIRYSRRIGNL
                     YTGSLYLGLTSLLENSKSLQPGDRIGLFSYGSGAVSEFFTGYLEENYQEYLFAQSHQE
                     MLDSRTRITVDEYETIFSETLPEHGECAEYTSDVPFSITKIENDIRYYKI"
     gene            9037..11478
                     /locus_tag="IXT20_002335"
     CDS             9037..11478
                     /locus_tag="IXT20_002335"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HAQ4860761.1"
                     /translation="MKEVVMIDAARTPIGKYRGSLSPFTAVELGTLVTKGLLDKTKLK
                     KDKIDQVIFGNVLQAGNGQNVARQIALNSGLPVDVPAMTINEVCGSGMKAVILARQLI
                     QLGEAELVIAGGTESMSQAPMLKQYQSETNEYGEPISSMVNDGLTDAFSNAHMGLTAE
                     NVATQFSVSREEQDRYALSSQLKAAHAVEAGVFSEEIIPVKISDEDVLSEDEAVRGNS
                     TLEKLGTLRTVFSEEGTVTAGNASPLNDGASVVILASKEYAENNNLPYLATIKEVAEV
                     GIDPSIMGIAPIKAIQKLTDRSGMNLSTIDLFEINEAFAASSIVVSQELQLDEEKVNI
                     YGGAIALGHPIGASGARILTTLAYGLLREQKRYGIASLCIGGGLGLAVLLEANMEQTH
                     KDVQKKKFYQLTPSERRSQLIEKNVLTQETALIFQEQTLSEELSDHMIENQVSEVEIP
                     MGIAQNFQINGKKKWIPMATEEPSVIAAASNGAKICGNICAETPQRLMRGQIVLSGKS
                     EYQAVINAVNHRKEELILCANESYPSIVKRGGGVQDISTREFMGSFHAYLSIDFLVDV
                     KDAMGANMINSILESVANKLREWFPEEEILFSILSNFATESLASACCEIPFERLGRNK
                     EIGEQIAKKIQQAGEYAKLDPYRAATHNKGIMNGIEAVVAATGNDTRAVSASIHAYAA
                     RNGLYQGLTDWQIKGDKLVGKLTVPLAVATVGGASNILPKAKASLAMLDIDSAKELAQ
                     VIAAVGLAQNLAALRALVTEGIQKGHMGLQARSLAISIGAIGEEIEQVAKKLREAEKM
                     NQQTAIQILEKIREK"
BASE COUNT         3778 a         2097 c         2524 g         3171 t
ORIGIN      
        1 gaaagaggtc gtgagcctgc cgtccagctc cgagtcacaa ggaacaagtt caaaaaacag
       61 cttctcatgt ttgttgaata ttgtgacttg tgaccgagga gttggcagga gaacaccgtt
      121 tgttcggaag aagggactgt gacaagattt ttgtcacagt ccctttaatc ttagtatatt
      181 tttctctact tgaataaaaa aattatagga ttggagaaaa taatcgagaa atttgttgct
      241 tgaataatag ccagttagac atttccttga ctgtttcagt tgtcatggat gaacaatgtg
      301 tctcatcttc tttgaactga gtagcaagtt cttctaaaaa ctcaggatcg taaatgacag
      361 cacttgtttc aaaattcaat cggtagctac gaatatcttg atttgctgaa cctaccatgc
      421 agatttcatc atccattatc aatgtttttg catggaggaa gccgttctca tagacaagga
      481 tttgtacatt ttccttgatc agctgccgag cataatattg tgttgctcga taaataaaag
      541 gatgatccgg tttgttggga atcatgattt tcacatctac accagaggca gttgcgattt
      601 ttaaagcagc aatgacactt tcatcaggaa caaggtaagg tgtctgtatc caaacacgtt
      661 ttttagcaga agtaatcaat ttgataaatg aaagcttgat ttgttccctt tcgttattcg
      721 gtccgcttga aacaagctgg atggatgtat cagcaagctt agattcatct cgatctgctt
      781 ttttaaagaa ataattcaaa tgatagccga cctttttttc ttcaggtacc gagacgttcc
      841 agtctgttaa gaagcgaagc tggagcaaag aagaagcggc accgaaaatc cgtatatgcg
      901 tatcgcgcca atagccgaat ttttttgttg tatttacata ttgattggca atattgaaac
      961 caccggtata actaatcttt ccatcgatca caacgatttt tcggtgatca tggtaattca
     1021 atcggaaacg aagtaatgcc ctttgtgagg taacaaatgt atggacatgt ccaccatttt
     1081 tgattagacg attgaaatct tttgcttttg tgccatgaga gccaaatgca tcatataata
     1141 ttcgaacttc cacgccttct gcggcttttt cttctaaaac atgtaagact tgctggccga
     1201 tattatctgt cacaaatgcg taatattcga tatggatcga atgtttggct ttttttagat
     1261 cttgaagcag tgaatccaat ttctcttgtc cgtctgtgag aagagtgaca gaattcattc
     1321 ttgtcagcgg catacgattt aatgaagaga agaagtcaac aaattgctgt ttgtctttat
     1381 cgcccatgtc aggatcgtaa tgttcaatgg tatctcctct aaaagactga aagttttcta
     1441 attctttcaa gtcactttgt cggagataga attttttctt atccgttaat ccacgtccga
     1501 aaaataaata taatacaaag cccaccccag gaagggcaaa caatactaag agccatgccc
     1561 aaatggctgc tacatctcgg ggtttgatca aaatagccac caaagcaata agtgcattta
     1621 atagataaag ggcggttata atactagata ccacaaaaat gttcctcctt acatacaata
     1681 acattagtat agcatgatag aagaaagaag cctaccaagg tgaatatact tttcactcct
     1741 ttcatgaaaa tgtaggtaaa tcgggctaaa gtagttgctt tttgtaggtt aataaggtag
     1801 gatattagta cagttgaata tctccttagg ggagtaaccg gcaacgtttg ttgcgataat
     1861 atcaacagta agttaaccaa atggttttct ggtattgtat gaaatggtga gacctaagac
     1921 aaatgcgtat ttgtcttagg tctctttttt ttcagacagg actagggcca agatcaaaaa
     1981 atgggggatg aaatttggaa aacaaggaac aagcacaaca agcggtctat aaagaaacga
     2041 tcgactcgtt gatgcagcaa acgcagagct caaccgatgg attgagcacc acgcaagcga
     2101 aggaaaaatt agaaagagat ggcttgaacc agctaaaaga aaagccgaag aagccaactt
     2161 ggaaattgtt cttagaaact ttcaaagatg caatggttat cgtattgctg attgtcgcac
     2221 tgattcagat ggtcatgggt gactacatcg aattcttagt tatttttgca gtattgatgt
     2281 tgaattctat aatcagtgtt gtccaaacaa aaaaagcaga aggttcttta gaagcgttga
     2341 aaaacctttc agcgcccgat gctcgagtga ttcgtgatgg taaggaaaaa acgattccgg
     2401 caaacgaact tgtggtagga gatatcgtgt tgttagacgc aggcgattat gtacctgctg
     2461 atggacgttt actagatgcg ggatcattaa aagtagatga aggtatgctg acaggagagt
     2521 cgacttctgc agaaaaagaa gtaacggata tcacaagtac ggtaccaatc ggagatcgta
     2581 caaatatggt attcagtgga acgatcgtaa cttacggacg cggtaaattc ttagtgactt
     2641 caactgggaa taatacggag atcggacaag ttgctggcct tttggaatca acaactgagc
     2701 aagtcactcc gttacaacga ggtctggata aattcagtaa aaaactaagt ttggctattt
     2761 tggcattgtc tttattgatt ttaggtatcc aactgtttcg tatttatctt ggagaaggta
     2821 caggagatat gactgcagat atcgtcagcg cagtgatgtt tgccgtagcc gtagccgtag
     2881 ctgctattcc agaagcatta cagtctatcg taacaatcgt gttatctttg ggaacgaata
     2941 aaatggcaaa acgtcatgcc attattcgga agcttccagc agttgaaaca cttggttcga
     3001 caagtatcat ctgtacagat aagactggta cattgacaca aaacaaaatg acagttgttg
     3061 actacttttt aggtaatggc gatactgggg atttcacaaa tgacccttca aaatggacag
     3121 ctgacgaacg tcgcttgatc gaaatcagtg ttttagcgaa tgatgcatcg atcagtgaag
     3181 acggtactaa attgggcgac cctacggaag tagcgtttat cgactttagt gaaaaattaa
     3241 accagcctta tcaggaaatc cgaaacaact acccaagaca agcagaactt ccttttgact
     3301 cagatcgaaa attaatgtct actggacaca caattgataa caaaaattac ttgtttgtta
     3361 aaggcggacc agatatcgtc ttccaacgca gtaaaaaagt attgctaaat ggtgaagtcg
     3421 ttccgatgac ggatgaattg atgaaaaaat tccaagagca aaatgaagct ttttcaacac
     3481 gcgcattacg tgtactagct tttgcttacc gtccacttga taattttgat ttatcatttg
     3541 aagatgaaga cgatttgatc cttgttggtt tgcttgctat gatcgatcct ccacgtgctg
     3601 aagtgactca ggcggtaaca gaagcaagaa gtgcaggaat caaaacaatc atgattactg
     3661 gtgaccacaa aacaacagct gttgctatcg caaaacaaat cgggattttt gaagaaggaa
     3721 acctggcttt gaccgggacg gaactagatg aattgacaga agatcaacta aacgaagttt
     3781 tagaaaaagt cactgtttat gcacgtgttt cacctgagaa taaaattcgt atcgttcgtg
     3841 cttggcaaaa taaaggtcat atctcagcta tgacaggtga tggtgtaaat gacgcaccag
     3901 cattgaaaca agcagacatc ggaattgcta tgggaacggg tacagatgtg gcaaaagatg
     3961 cagcagcgat ggtattgacg gatgataact ttgcttctat tgtcagcgca gtagaagtag
     4021 gacgtaatgt gtttgataat atcaagaaag ctatttctta tctatttgca ggaaatttag
     4081 gtgcgatcat tgcgatcatc tttgccttag ttgctgactg gtccaatcca tttacagcat
     4141 tacaactgtt gttcatcaat ttagtcaatg attcactgcc agcaattgca ttaggtacag
     4201 aaaaagctga acctgctacg atgaaccgcc cgccaagaga tccaaattca ggaatcttta
     4261 cggacaaaac attgatttct gtaacgtatc gtggtgtgct gattgggatt gctgttatct
     4321 ttgctcaatg gcttggcatg cagcattcac ctgaattcgg tgtagccatg tcattttcaa
     4381 cattgatttg gtgtcggaca ttgcaaacat tcccagcacg ttcaaacaca caaacagcta
     4441 tccaagcagg gttcttctct aacagaagtg tcttactagc tgtagttgta tgtagcgcgc
     4501 tttactgctt aacaatgatc ccaggactga aagaagtctt ctctatccca tcagcatttg
     4561 gattgacaga agtctttaca tgtatgggaa tcgcacttgt gacaattgtt ttgatggaaa
     4621 tcactaaatt gatcattgta cgagtacaag gaaataaata aaacgaatca acgaggttgt
     4681 gagcctgccg tttaattctg agtcacaagg tgcaatttca acaaacagct tctcatgttt
     4741 gttgaatatt gtgacttgtg accgaagagt tggcaggaga acaccgttta aggaagaagt
     4801 tgtaaaatca gcattttgag ctaatactga gggtcatctg attggacact gtttattcgg
     4861 aaagagggac tttgacacga ctttggtcac agtccctttt tttatttatg atagacggaa
     4921 agccaatcat tttattattc atcaaaagag atagaataga taaaagagga gaatgattta
     4981 ttccgaaaag tgaaaacata taacaaaaaa ttagaggaaa tcaaatgaat gaaaaagaat
     5041 gggatgaatt tgctgaagaa tattatgaaa tccagcaaga atcgcaagta actattgctg
     5101 aagatgtaaa aaattatctg aaaacggaga aagtcctgcc tgctgaaaca gcagcagacg
     5161 ttgctggagg atctggcaga tttctttcct tagcaaaaga aattaatcgc tatgatttga
     5221 tcgatttttc ttcaggaatg ctagaaaatg caaaaaaaga agcagaaaaa aatgagttgc
     5281 caaatgtttc ctttatcaaa cagtcgttag aagaatttat ccaatcaaat gagacatatg
     5341 aaatggtttt tacagcagca aatccagcat tggtacatct tattcaatta gaaaaattga
     5401 tgaaaaagtc aagcaaatgg tgcgtgataa tcagagtagt ggaatcaatc gatgatgttt
     5461 tttatcctat cgaaaagcaa ctaggaatga tagaagaaga tcctaataca tcgccagttg
     5521 taatggatca atttgaaaca tacttaattg atgaaggatt caaagtgaag aaaaaagatt
     5581 ttacttattt tacacaggaa tcaattactg aagaattgat gaagctatac tatgacgcgt
     5641 acgcaaaaga tcctgttgtg accaggtaca ttcgcaaact attcagtgaa aaacgagaaa
     5701 tccttagtac gactaagctg acctatagac tgctcctttg gtccgcagat aaaaaaagaa
     5761 cctttcacga ctcgtgaagg gttcttttta tgatgtgcgc cctgcatggg tgaaaacttg
     5821 gtggtgaaag tccactacag gcttggcagt aggaactgtt agcgaaagac aaggcggcaa
     5881 tcgtgaggtt gtggctgaag gaagtcggac gcaaactcct gaactgacga acagaaacta
     5941 gatacaaggc tgaattaggt cggataagtc ttcatcacaa gacaaagtcc aatactgccc
     6001 gaaacctata cagtaaatct agcagttaca tgggaggaaa gttttcactc ttaccggggg
     6061 agatctcatg agggtgttgt gggcgttgaa tgaaaacaaa ccagcacaac gatcatggcg
     6121 acatgatggt gaatcatgag aagtcagccg aggtcatagt agtaacgaca ctgaccgtta
     6181 cgaaggactg aacaataaga atcttgaata atttaggagg tgtgagaaga tgcgacgacc
     6241 gcagaaaaca agaaaacttg gctatcatca aagagctagt gtggaacacg aagggtatga
     6301 tgaagtgcgc agcacttctt gcggtgaaca gaggaaacaa gatggtgtag aaaatctgtt
     6361 tgggagaatt ctagatcgga ataatctcaa tcaagcgtat cttcaagtag tcagaaataa
     6421 aggggcggct ggtgcagatg ggatgaccgt cgatcaactg cttccttact tgaaagagca
     6481 ccgcgaggaa ttactatcca atttacgcca agggaactat aaacctcagc ctgttttgcg
     6541 agtagaaata ccgaaaccaa acggcggtgt aagaaaacta ggaattccca cggttattga
     6601 tcgtatgctt caacaggcaa tcaatcaagt gttacaaccg ctatttgaac cagaattctc
     6661 agataatagt tatgggtttc gcccaaatcg cagcgcacat gacgcagtga agaaagcaaa
     6721 atcttattat gaacaaggtt accgatatgt ggtggacatt gatatgaaag cctactttga
     6781 caccgtaaat cacgataagc tgatgtattt cattgagcgg aaagtaaagg acaaacaagt
     6841 cctaaaactg atccgtcaat acttaatgag tggtgtaatg gaaaatggac tcatcacaac
     6901 aactgaagaa ggaacgcccc aaggaggcaa tctctctccc ctgttaagta acatctacct
     6961 gaacgaattt gatcaagtgc tagaaaatct aggacataaa tttgttcgat acgccgatga
     7021 ctgcaatatc tatgttagaa gcaaacgagc aggacagcgt gtgatgaaga aatccattca
     7081 atttctcgaa gaacaactaa agttgacggt aaatcgagaa aagagtgcgg taggaagtcc
     7141 attgaaacga aagtttcttg gattctgcat tctgccaaca aagaaaggta ttaaaattcg
     7201 ccctcacgca aaagcaaaac agcgtgtgaa aagcaagctc aaacaactca ctaaaagaaa
     7261 ccgtggaaga agtatccaac ttattttaaa agaaatcaag caactaatga ctggttggat
     7321 caactactat ggaattggtg agatgaaaga attcatgagg gagttaaacg gttggttgaa
     7381 gcgaagaatc agacaatata tttggaaaca atggaagaat ccaagaacca aaagaaaaaa
     7441 tctgattcgt ttagggatcg ataaagcgaa agcgtatgaa tggtcaaata caagaaaagg
     7501 tatttggtca gtctccaaaa gtcatattct tcatcgatca ttaacagaca aagaactggc
     7561 ccgccaagga tatgaggaca tctccttgaa gtaccagttc gtacactcaa cttattgaac
     7621 cgccgtatac gggtccgtac gtacggtggt gtgagagggg cggaaggtca gttggctttc
     7681 ccctctactc gattttatta tattttgtag taacgaatat cattttcgat ctttgtaatc
     7741 gaaaaaggta catctgaagt atattctgca cattctccgt gttcaggaag cgtctcactg
     7801 aaaatcgttt catattcgtc aacagtgatc cgtgttcgag aatctagcat ctcttgatga
     7861 gattgtgcga agagatattc ttgatagttt tcttccagat aacctgtaaa aaattcactt
     7921 actgcaccag aaccgtagct gaatagaccg atgcgatctc ctggctgtag tgatttcgaa
     7981 ttttccagta gagaagttag ccccaggtat aatgaaccag tgtaaagatt accgattcgt
     8041 cggctgtaac ggatgctttc ttcatagcga gccataagac gttcctgatt ttcttcgtct
     8101 gtttggtcta atacgctttg caatgccttt tttcccatct tagtatacgg aatatggaaa
     8161 gcaatcgcct gataatcttc gagtcctcga cccgacaatt ctttatgtcg attccaaact
     8221 ttttggaatg attcgatata cgtagaatta gataaaggac catcaacaac aggaaattcg
     8281 ctataatctg gacgccagaa atcatagata tcttctgtca gaaatacgct gtcgtcttca
     8341 atcgataaaa tacgcgggtt ttgagtgatc atcatcgcaa cagcaccgac accttgcgtc
     8401 acttcaccac cgcttgccaa gccgtaacga gcaatatcac ttgctatgac tagtactttt
     8461 cgttctggat gatttttgac atattctttc gccatgtgca aagctgcggt gccaccgtag
     8521 caagcctctt ttacttcaaa agaacgagca aaagattgga ttttcagtaa atggtggata
     8581 attaccgcac tcgcttttga atgatcaatc ccggattctg ttccgacgat gaccatgtcg
     8641 attagctcac ggtctttttc tgttacgatt ttgctggcag catttgctcc tagtgtaatg
     8701 atgtcttcat ttgcacgatt gactgccatt tggtcttgac caatccctat atggtatttt
     8761 gcaggatcat ccccacggct ttctgccaac tctgtcatat ctaaatataa attaggaata
     8821 aaaaaggaaa gacgatcaat ccctattttc atttcaacac ctctttactt atgataaaaa
     8881 acttacttta ctaaggtatc ataaaaagat taaaagaaat ataaaaaaga gaatatttaa
     8941 ggattgttaa aatttaatct tagaaaaaaa taactggaac aagagctatt tgtttttaca
     9001 gcaaagaatg gtatattttt gtaggaggtg gcttacttga aagaagtcgt tatgatagat
     9061 gctgcaagaa cgccgatagg aaagtatcgc ggaagtttaa gtccatttac tgcagttgaa
     9121 ttaggcacac ttgtgactaa aggattatta gataaaacaa aactgaaaaa agataaaatc
     9181 gatcaagtaa tatttggaaa tgtcctgcaa gctggtaatg gtcaaaatgt tgccagacag
     9241 attgctttga acagcggtct ccctgtagat gtcccagcaa tgacgatcaa tgaagtttgc
     9301 ggatctggta tgaaagcagt gatcttagca cgccagctga tccaattagg tgaagcagaa
     9361 cttgtgatag ccggcggaac agagagtatg tctcaagcac ctatgctgaa acagtatcag
     9421 tcagaaacaa atgaatatgg tgaaccaatt tccagtatgg tcaacgacgg attgactgac
     9481 gcattttcaa atgcacatat gggattaacc gcagagaatg ttgcaacaca attttctgtg
     9541 agcagagaag agcaggatcg ctatgccttg tcgtcccagt tgaaagcagc acatgctgtc
     9601 gaagccggtg tattttctga ggagatcatc ccagtcaaga tttctgatga agacgtgtta
     9661 tctgaggatg aagcagttcg tggaaatagt acattggaaa aactgggcac gttacgtaca
     9721 gtattctcag aagaaggaac tgtaacagca ggaaatgctt ccccgttgaa tgacggtgcc
     9781 tctgtggtga tccttgcatc caaagaatac gcagaaaata ataatctgcc ttatttagca
     9841 accatcaaag aagtagcgga ggtcggtatt gacccaagta tcatgggaat cgctccaatc
     9901 aaagccattc aaaaattgac agatagatca ggaatgaatt tatcaacaat cgatcttttt
     9961 gagatcaatg aagcatttgc agcttcttct atcgtagtta gccaagaact gcagttagat
    10021 gaagaaaagg tcaatatata tggaggagca atcgcattag gccatccaat tggtgcaagc
    10081 ggtgcacgta tcttgactac attagcttac ggattgcttc gtgaacagaa gagatacggt
    10141 attgcttcat tatgtattgg tggaggttta ggcttggcag tcttgttaga agcgaatatg
    10201 gagcaaacac ataaggatgt gcaaaaaaaa aagttttatc agcttacgcc atctgaacgg
    10261 cgcagtcaat taatagaaaa aaatgtattg acacaagaaa ctgcgttgat ttttcaggaa
    10321 caaacacttt ctgaagagct ctccgatcac atgatcgaaa accaagtgag tgaagtggaa
    10381 atcccaatgg ggatcgcgca gaattttcaa atcaatggca agaaaaaatg gattccgatg
    10441 gcaacagagg aaccatctgt cattgcagct gcaagtaatg gtgcaaaaat ctgtggaaat
    10501 atctgcgcag aaacacctca gcggctgatg cgagggcaaa tcgttctatc aggaaaatcg
    10561 gagtatcaag cagtgatcaa tgcagttaat catcgaaaag aagagctgat tctttgtgca
    10621 aatgaaagtt atccttcaat cgtgaaacgc gggggcggtg ttcaagatat ctctactcgg
    10681 gaattcatgg ggagtttcca tgcatactta tctattgatt ttttagtgga tgtaaaagat
    10741 gcaatgggcg ctaacatgat caactcgatt cttgagagtg tggcaaacaa gttacgggag
    10801 tggttccctg aagaagagat tctattcagt attttaagta attttgctac agaatcattg
    10861 gcttctgcct gttgtgaaat cccattcgaa cgcttaggga gaaacaaaga aatcggtgaa
    10921 caaattgcca aaaaaatcca acaggcaggg gaatatgcga agcttgaccc ttaccgtgca
    10981 gcaacgcaca acaaagggat catgaatggt atagaagctg tagtggcagc tacaggaaat
    11041 gatacacgtg cggttagcgc ttcgatccac gcctacgcgg caagaaatgg attataccaa
    11101 ggattaacag actggcagat caaaggggat aaactagttg gaaaactaac ggtgcctttg
    11161 gctgttgcca cagtaggcgg agcttccaat atcctgccaa aagcaaaagc atctttagct
    11221 atgttagata ttgattcagc caaggaacta gcacaagtaa ttgctgcagt agggttagct
    11281 caaaacctgg ctgctttgcg tgcattagtt acagaaggaa tccaaaaagg ccacatggga
    11341 cttcaagctc gttcattagc gatctcaatt ggggctattg gtgaggaaat cgagcaagtt
    11401 gcgaaaaaat tacgtgaagc agaaaagatg aaccagcaga cagctataca aatattggaa
    11461 aaaatccggg aaaaataaaa aagcaatatc tttagaagaa taaagcaaga tttacagtta
    11521 ttttatttac tgcagatctt gcttttttct ctatcagaaa atatgtttat
//
LOCUS       DACGLE010000081        11416 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0534
            SAMEA3304449-rid9560453.denovo.085, whole genome shotgun sequence.
ACCESSION   DACGLE010000081 DACGLE010000000
VERSION     DACGLE010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304449
            Sequence Read Archive: ERR985064
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11416)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11416)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702225.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702225.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 20:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,045
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 27 of 67
            Pseudo Genes (incomplete)         :: 25 of 67
            Pseudo Genes (internal stop)      :: 19 of 67
            Pseudo Genes (multiple problems)  :: 4 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11416
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304449-rid9560453.denovo.085"
                     /strain="VREN0534"
                     /db_xref="taxon:1352"
     gene            complement(361..1479)
                     /locus_tag="IXT20_002336"
     CDS             complement(361..1479)
                     /locus_tag="IXT20_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4860762.1"
                     /translation="MAKKDKELLTQLTSEGAKGPFVTIMLNTHVAHQDVEKDQIKFKN
                     FAKEAKKRFEKKYSEHDWSPFQEKIENLLGDPSFWRSATTSVAVIITADQTYIHRLSI
                     PVDDQYYVSDLPYLLAIIKNGQFNYSYYLLGLNRDSMKLYEVSNKQVKEVSLPEGAPV
                     DVVTALGSELTGGGLNFSSNGGGGARDGVTFHGVNPKDEEVEIDWVNYYQAVDTYLKD
                     SFENPEQWPIYLFALPENQTMFKKIAKNPYYCADVAISASPAQLSFRDIEEGAKKLSS
                     ELAAQESKEYNKLLDKKFIDQLVDIVPAAKEGKVSHLFIATSNLADGFGEDPETEYDR
                     RQVLNALADDVIINGGKVFILDQKDAPDEKSLTAILRY"
     gene            1950..3257
                     /locus_tag="IXT20_002337"
     CDS             1950..3257
                     /locus_tag="IXT20_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HAQ4860763.1"
                     /translation="MSIKKTRLVLLLASLILCLASFLPAVAVQAEDTFKVNAKAAFAV
                     DAQSGKILYDQDGEKTMGIASITKIIGLYIVLDQVKEGKLSWGDKVSISDYAENLSIT
                     PDLSNVPLHKENTYTVKELFDSAIIQSANASMVALAEKISGSEAKFTERMKEQLKDWG
                     IKDATIVNASGLNNSYLGENRPEGTGENDENQMSAQDVAIVARHLILDFPEILDVSST
                     TTQMFGENTQSPVEMVNWNWMLPGFVNYKEGVDGLKTGTTELAGACFVGTITKNDQRI
                     ITVVLNADGHAENPSARFNETDRLMDYCYDNWSEKELGKANASIPSLKTIDVKDGKES
                     TVPVVLKSPVKVWVRNDMDTGKLTITPTLDKKQVTDNEIQAPMDKGTKIGTATITLAD
                     DKLGYLEDNNAPSTEIITDKTVEKANIFVIGWRRVAEFFGNLF"
     gene            3376..4575
                     /locus_tag="IXT20_002338"
     CDS             3376..4575
                     /locus_tag="IXT20_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HAQ4860764.1"
                     /translation="MNLTRDLIFLVLYLIGVFSIVYWRKQKKDPSLFFYGSTWTFTVI
                     LTYFVVLEIFYSTFYFLIPLLFFSIFAFSYFTEKRRLLNGLLFNVLLISFGIYLFVLL
                     YETQNIFLGGLIALITIPLLLVLLFGIYGLIVFLFWNGVTVLRRESHSLANLLTLILA
                     IFLTLFLVFDFFLLKYLPQWINALFFCVPLILIYLFIVFYNFLTVSFLYQFNRPRYNQ
                     DFIVVLGAGLINGETVSPLLAKRINKAIAFYRAQSRATLNPPILLMSGGQGADEKVPE
                     AIAMKQYAMEQGIPERDILVETNSTTTLENMLYSKEIMDQQMKGPYRAIFSSNNYHIF
                     RAGLYARQAKLKANGIGAKTAFYYLPNAFLREYLAILMMNKKRHMIICGLLFAGSAFL
                     SLITLLF"
     gene            complement(4602..5870)
                     /locus_tag="IXT20_002339"
     CDS             complement(4602..5870)
                     /locus_tag="IXT20_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated chloride channel family protein"
                     /protein_id="HAQ4860765.1"
                     /translation="MEKQLPSPVRILVYMLKWLVITAILGIFMGSLSAFFLNSLTVVT
                     DIRLSHPWLLCLLPVSGALFAYLYAYHGGLSSRGNNLVIDQGNGGEEKIPLRLIPLTL
                     FGTITTHLFGGSVGREGTAVQMGGALADNTARLFRLDKSEREILIISGISAGFSSVFG
                     TPLAGTLFGLEVLAIGKVRTEAIFPSFFAALFANFMTESYGVSHLHYQMGVIPEWSML
                     LFFKVFVAGIAFGLIGWVFSRSIVFLKARYAQWIPHPVCRNTIGGAVVVAAALILQTQ
                     RYLGLSLPLLQESFNGQAHVYDFIGKLFFTVLSLGAGYQGGEVTPLFEIGATLGAALA
                     PLLHVSVPFLAGLGFIGVFSGATNTPIACFVMGIELFGSQAAVYFFMICLISFMCSGN
                     SGIYSAQQVAVKKGILFLPIVKRMKEKNKV"
     gene            complement(6033..6679)
                     /locus_tag="IXT20_002340"
                     /pseudo
     CDS             complement(6033..6679)
                     /locus_tag="IXT20_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728800.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FusB/FusC family EF-G-binding protein"
     gene            complement(7087..7590)
                     /locus_tag="IXT20_002341"
     CDS             complement(7087..7590)
                     /locus_tag="IXT20_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAQ4860766.1"
                     /translation="MTEKWREDEEYMSYVEDLLETEAVKKLANYTQHVHSTRLEHSIS
                     VSYYSYLLAKKWGGNAKATARAGLLHDLFYYDWRTTKFDEGTHAYIHPRIAVKNAEKI
                     TELSDLERDIILKHMWGATIAPPKYKEGYIVTFVDKYCAVKEAAQPMSASMRKRWQRY
                     FGKESSI"
     gene            complement(7713..8477)
                     /locus_tag="IXT20_002342"
     CDS             complement(7713..8477)
                     /locus_tag="IXT20_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HAQ4860767.1"
                     /translation="MKEIQSAKNPRIKELKKLHKKKYREEKEEYLLEGFHLIEEAASS
                     DAEIKELFVTQRGMKEWGDWLAANKEISTFLVSDEVMDAVSDLPTPQGILAVMALTAE
                     GTPDFSGGWLLLDNVQDPGNVGTMIRTADAFDMAGVLLGKGTADIYSTKVLRSMQGSN
                     YHLPVLQKDLTEVISLMKDKGFTVYGTELNEEAHALDKVEKSDNFAIIMGNEGQGVSQ
                     EILSLTDQNVYIPMKGNAESLNVGVAAGIVLYHFSL"
     gene            8585..8860
                     /locus_tag="IXT20_002343"
     CDS             8585..8860
                     /locus_tag="IXT20_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HAQ4860768.1"
                     /translation="MRKVKMNVQGRVQGVGFRYMTKMVADQLGVTGTVKNEDDGSVTI
                     EAIGNDDIIQKFIEEVKKSPSPSGRVQYVDVQEDPMIEERKKFDVVG"
     gene            9298..10248
                     /gene="yidC"
                     /locus_tag="IXT20_002344"
     CDS             9298..10248
                     /gene="yidC"
                     /locus_tag="IXT20_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HAQ4860769.1"
                     /translation="MNKWKNWLLGSGLVSILLFLSGCVRMDSNGNPDTSGIVYRVLVH
                     PMGQAITYLVENFNWSYGWAVIAMTVIVRIIILPLGISQSKKTMIQSEKMQALKPQVE
                     AAQQKLKAATTREEQMAAQAEMQQVYRENGLSMTGGIGCLPLLIQMPIFSALYFTARY
                     TEGIRESSFYGIDLGSPSMVLVAIAGVAYLLQGYISTIGIPEEQKKTMRTMLIVSPAM
                     IVFMSISAPAGVTLYWVVGGVFSCLQTFITNVMMKPRLKAQVAEELKQNPPKQVVTPR
                     IDVTESAKPADSKQATKQIKQPKNSTNNKGRNAGKQQKRK"
     gene            complement(10427..10627)
                     /locus_tag="IXT20_002345"
     CDS             complement(10427..10627)
                     /locus_tag="IXT20_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4860770.1"
                     /translation="MGGISALSFMENGVLCGRATWAKGVAPFVTEGEEKASSWMLEEG
                     KQNIKELSTLLEETATPVYVDR"
BASE COUNT         3437 a         2397 c         2016 g         3566 t
ORIGIN      
        1 acggtgttcc agaagtagct tcttcggaaa taagccgaaa tttcacaaaa atttgagaaa
       61 taatttttgt caatctcttc ttatttcttg aagctgacca cttctgtcac aacctcttcc
      121 ttaaacggtg ttcaaaagct tgcatctgcg gtaataagcc caccaaacaa aaaatatgaa
      181 gagctatttt tgcttgctgt tcttattact tgatgcagaa cagctttttc acaacctctc
      241 atacacggtg tttaatcagc tgaccttcgg tattagctca aaaaacggaa aaatatgtga
      301 aactattttt ccgtttgttt ctctaatact caggtcaaaa cactgatttc acaacctctt
      361 ttaataacga agaatcgctg tcagactctt ttcatctggc gcatcttttt gatccaaaat
      421 aaacactttc ccgccattaa tgatcacatc atctgctaat gcattcagca cttgacgccg
      481 atcatattct gtttcaggat cttcaccgaa accatcagct aagtttgacg tagcgataaa
      541 taaatgtgac acttttcctt ctttcgcagc tggaacgata tcaactaact gatcgatgaa
      601 tttcttatct aagagtttat tgtattcttt tgattcttga gctgcgagtt cacttgacag
      661 cttcttcgcc ccttcttcga tatctctaaa ggatagctgc gcaggagaag cagaaatcgc
      721 aacgtctgca caataatagg gatttttagc aatttttttg aacatcgtct gattttctgg
      781 taatgcaaat aaataaatcg gccattgttc aggattttcg aaagaatcct tcaagtacgt
      841 atcaacagct tggtaatagt tgacccaatc gatctctact tcttcatctt ttggatttac
      901 gccatggaaa gttactccat ctcgggctcc gcctcctcca ttagaagaga agttcaaacc
      961 gccacctgtc agttcagatc ccaatgcagt aacgacatca accggtgccc cttctggtag
     1021 agatacttct tttacttgtt tgttgctgac ctcgtacaat ttcatggagt cgcggttcaa
     1081 tcccaataaa taataagaat agttgaactg cccatttttg ataatagcca gtaaataagg
     1141 aagatcactt acatagtatt gatcgtctac cgggatactc aaacgatgga tataggtctg
     1201 gtcagcagtt ataatgaccg ctacactcgt agttgcacta cgccaaaacg aaggatctcc
     1261 taacaaattt tcaatttttt cttggaacgg ggaccagtca tgttcggaat attttttctc
     1321 aaaacgtttc tttgcttctt tcgcaaaatt tttgaatttg atctgatctt tttccacatc
     1381 ttgatgggcg acatgcgtat tcagcatgat cgtgacgaat ggtcctttag cgccttctga
     1441 agtcaattgt gtcaataatt ctttatcctt ttttgccata aacgtacccc tcctaaaaaa
     1501 atcattcctt tgtttctatc taaagtctac cacaacacta tttttccata caataaaacg
     1561 tatctaaaaa aataaaacgg tttctacggt tttaaattta gctatttttt taagttatct
     1621 tcataatttc ctcaattatt tagccccagt cttcaactat tcttcatgaa atgctgataa
     1681 actaagaaaa agaataaaag aggtggatct tatgcatttg attatgattt tcagtttatt
     1741 aagtattatc agtttatttg ctttcagcgc ttcccgctta ttagatgcgg aaacacagcc
     1801 tattcctgta aaagtacgta aaaacgaccg ctaaacttcc ataacgcgcg ttcttatcct
     1861 aaagtttttt cagaaacatt gaaactctct cccctctctg ataaaatttt aatttgtagc
     1921 gaaacaaatt aattacaagg agatcggata tgtccataaa aaaaacacgt ctagtcctat
     1981 tattggctag tctcattctt tgccttgctt cttttctacc agctgttgct gtgcaagctg
     2041 aagatacgtt caaagtaaat gcaaaagcag cttttgctgt agatgcgcaa agcggtaaga
     2101 ttctttatga tcaagatgga gaaaaaacca tggggatcgc ttcaatcaca aaaatcatcg
     2161 gtctttacat cgtccttgat caagtaaaag aaggaaaact gtcatggggt gataaagttt
     2221 cgatttcaga ttatgcagaa aatttgagta tcaccccaga tttatcgaat gttcctttgc
     2281 ataaagaaaa cacctacact gtcaaagaat tattcgactc tgcaatcatc cagtcagcca
     2341 atgcttcaat ggtggccttg gcagaaaaaa tctctggcag cgaagctaaa ttcactgaac
     2401 gaatgaaaga acaattgaaa gattggggaa tcaaagatgc aacaatcgtt aatgcatcag
     2461 gattgaacaa ctcttatctt ggcgaaaacc gaccagaagg cactggagaa aacgacgaaa
     2521 atcaaatgtc tgctcaagat gtggccatcg ttgcccgcca tttgatcttg gatttcccag
     2581 aaatactgga tgtatcaagt actacgaccc aaatgtttgg cgaaaatacg caatcaccag
     2641 tggaaatggt caactggaac tggatgctcc caggattcgt taattacaag gaaggcgtgg
     2701 acggattaaa aactggtacg actgagcttg caggtgcttg tttcgttggt acgatcacta
     2761 aaaacgatca acggatcatc actgtcgtat tgaatgcgga cggccatgcg gaaaacccga
     2821 gtgcccgctt caatgaaaca gaccgtttga tggactactg ctatgacaat tggtcagaaa
     2881 aagagctcgg aaaagctaac gcaagtattc ctagtctaaa aaccattgat gtaaaagacg
     2941 gaaaagaatc aacagttcct gttgtgctta aaagccctgt aaaagtatgg gtacgaaacg
     3001 atatggatac tggcaaatta acgatcacgc caacacttga caagaagcaa gtcactgaca
     3061 atgagatcca agcacctatg gataaaggta caaaaatcgg taccgctacg atcaccttgg
     3121 cagacgataa attaggctat ttagaagaca acaatgcacc atcaacagag atcattacgg
     3181 ataaaacagt ggaaaaagcg aatatattcg tgattggctg gcgacgcgtc gcagaatttt
     3241 tcggcaactt gttttaattg aacaataaga aaaacatctt tccatcggaa agatgttttt
     3301 ttgctttttc tagtttaaat aacagctttt ttcatctatt ttcgttatac tgtataagaa
     3361 aaaggagttg aatttatgaa tctaaccaga gatctgatct ttttagttct ctatcttatt
     3421 ggggtattct ctatcgttta ttggcgaaaa cagaagaagg atccttcctt gttcttttac
     3481 ggcagtacgt ggacatttac ggtaatctta acttattttg tcgtgttgga aattttttat
     3541 tcgacctttt attttttgat tccattgttg ttctttagta tctttgcttt ttcttatttc
     3601 acagaaaaac gaaggctact gaatggtctt ttattcaatg ttttactcat cagtttcggc
     3661 atctacttgt ttgtcttgct gtatgaaacg caaaatattt ttcttggcgg actgatcgcc
     3721 ttgatcacga ttccattgct acttgtcctt ttatttggta tttatggatt gattgttttt
     3781 cttttttgga acggggtcac agttttacgt agagaatcgc attcattagc taatctttta
     3841 acgttgatac tagccatttt tcttacactg tttcttgttt ttgacttttt cttgttaaaa
     3901 tatttgccgc aatggataaa tgctttgttt ttctgcgttc ccctgatctt gatctatcta
     3961 tttatcgtat tctacaactt tttgactgtc tcattcttgt atcagttcaa ccggcctcga
     4021 tacaatcaag acttcatcgt tgtgttaggc gctggattga tcaatggaga aacagtttct
     4081 cctcttctag ccaagcgaat caataaggca attgcttttt atcgggctca aagccgtgct
     4141 acattaaatc cgcccatctt attgatgtct ggcgggcaag gagcagacga aaaagtacca
     4201 gaggccattg cgatgaaaca atatgccatg gaacaaggaa tccctgaaag agacattctt
     4261 gtcgaaacca attcgacaac cactttggaa aacatgctct attcaaagga aatcatggat
     4321 cagcaaatga aagggccata ccgcgcgatc tttagttcca acaattacca catttttaga
     4381 gccggtcttt atgcgcggca agccaaattg aaagcaaatg gtattggtgc aaaaacagct
     4441 ttctactact tgcctaatgc gtttttaaga gaatacctcg ccatcttgat gatgaacaaa
     4501 aaaagacata tgatcatttg cggattgctt ttcgcaggct ccgcattctt atcgctgatt
     4561 actttacttt tctaaaaaaa caaggtgtga caaaacatat gtcacacctt gtttttttct
     4621 ttcattctct taactattgg caaaaagagt atcccttttt tgaccgctac ttgctgagca
     4681 gaataaatac cgctgttgcc tgaacacata aagctgatca agcagatcat aaagaagtat
     4741 actgccgctt gtgaaccgaa caattcgatc cccatcacaa agcaggcaat cggtgtattt
     4801 gtcgcaccag aaaatactcc gataaagccc aatcctgcaa gaaaaggaac agaaacatgg
     4861 agcaaaggcg ctagtgctgc accaagcgtt gcaccaattt caaacaatgg tgtcacttct
     4921 cccccttgat atcccgcgcc taaagacaaa actgtgaaga ataacttccc aataaaatca
     4981 tagacatgtg cctgtccatt aaatgattct tgaagtaacg gaagactcaa tccgagataa
     5041 cgttgtgttt gcaaaatcaa tgctgctgcc acaactacag ctccacctat cgtatttcga
     5101 caaacaggat gaggaatcca ttgtgcatat cgcgctttta aaaagacgat cgaacggctg
     5161 aatacccaac caatcaaacc aaaagcaatc ccagcaacaa aaactttgaa gaacagcaac
     5221 atactccatt caggaatcac acccatttgg taatgtagat gagaaacacc atagctttct
     5281 gtcatgaaat ttgcaaacag tgcagcaaag aagctaggga agatcgcttc tgttcggact
     5341 ttcccgattg ccaacacttc taatccaaat aatgttccag ctaaaggtgt gccaaataca
     5401 gaactaaaac ccgcactgat tccactgata atcaagattt cccgttctga tttatccaat
     5461 cggaacaaac gtgccgtatt atctgctaac gctcccccca tttggactgc tgttccttca
     5521 cggccaacag atcccccaaa taaatgggta gttatggttc caaacagtgt aagtggaata
     5581 agccgcaacg ggattttttc ttctccacca tttccttgat cgatcactaa gttgtttcca
     5641 cgactagaaa gtccgccgtg atacgcatag agataagcaa aaagcgcacc actaactggt
     5701 agtagacaaa ggagccaagg atgtgatagt cggatatctg tcacaactgt caggctattt
     5761 aagaaaaaag cagacaagct ccccatgaat ataccgagaa tcgctgtgat caccagccac
     5821 ttcagcatat acactaaaat gcgtactgga ctcggtaatt gtttttccat aaaaaattcc
     5881 tcctcaataa aaaaacgcct actaaagaaa ggggctgtcc ccagacttta gtaggcgtca
     5941 ttagctatct ttcgttagca tttaaaggcg aacaccatca cctattcact tccattaaaa
     6001 gatagcatat ttttctgtaa aaaacaataa aattatcgtt ctttctttac acgttcagca
     6061 aattcataga aaggagccaa tttcgttaac tgttgattac attggtcact atcatgacaa
     6121 atataattcc cctttttcgt atacgtgcca tcagaacctg ctttcgttgt agaaagaaaa
     6181 agcgaaacat tcgatgtttt ctgacagatt gcacagattc ccttatttat cgttggtgat
     6241 aaagtaccgc taatcccttt taatttttca tcttcgtaat agaccatgaa ttttttctgc
     6301 gtacctggat cattccagcc gatgtacgta tgttcttgca agtcgatttc attccaagct
     6361 ggttgtttga gttttttcac ttttcggaat aatttctcga tttgtggttt gctaggctgc
     6421 ataaacggct gaacgatttg cttcagctcc tccaatgctc tttctgcttg cgcttttgtc
     6481 agttttacgt ctaaaactgt cgttaaaaat gtatcgattc tggcagttcg tgatctaata
     6541 tgtctcgaat cttttcagct gctagtgcct gaactgtttg taccgttttt ggatcattga
     6601 cagatgagta cgcatttaat aactgttcta cttctcttat tataaaaaaa tattggtatg
     6661 gttttattgt ttgttccatg attttaaagt cctttcttct aaccaaagtg catactttgt
     6721 agaaaaaagt gatcttctgc aaagtatgtg ttacctgaag atcccggact cttttaaata
     6781 agatacgttt tgcatcttat catcaccccc tttaatgctt attattctat gtacatgatt
     6841 cattcgataa ataaaagtaa accgtatctt gaacagatag tcaactgcaa acagaaaaaa
     6901 ggactgtgac aaaagtcgtg tcaaagtccc ttgtttcgaa tgaatggtgt tcaaaagctg
     6961 gcatctgcgg taataagccg aaacttcaca aaaatttgag aagcaatttt cgtaaattcc
     7021 ttcttatttc ttgaagctga ccacttctgt cacaacctca atatggtcta ttattggttt
     7081 tatctattaa atcgaggatt ctttcccaaa gtaacgttgc catctcttac gcatacttgc
     7141 tgacataggc tgagcggctt ctttgactgc gcagtatttg tctacgaatg ttacgatata
     7201 tccttctttg tacttaggcg gtgcaatcgt tgcgccccac atgtgcttca aaatgatatc
     7261 tcgctcaaga tccgacaatt cggtgatctt ttctgcattc tttacagcaa ttcttggatg
     7321 gatatacgca tgtgtgcctt catcaaattt cgtcgtacgc caatcatagt agaacagatc
     7381 atgcaaaagt ccagcacgcg ctgttgcttt agcattgcca ccccattttt ttgcgagcag
     7441 ataactatag taagaaacac tgatggaatg ctccaaacga gtggaatgaa catgctgtgt
     7501 gtaattcgct aattttttca ctgcttctgt ttctaaaaga tcttctacat aagacatata
     7561 ttcttcgtct tcacgccatt tttctgtcat aattatcgat ctgccacctt tttgaatttc
     7621 attttcttct gaaattgatc taagtatacc accagtgttc ggattattct agtcgccttg
     7681 gataaaatag ccaaagctta atagttcctt aatcataaag aaaaatgata aagaacaatc
     7741 cctgccgcaa ccccaacatt caacgattcc gcatttcctt tcatcgggat atatacgttc
     7801 tgatcagtca aagacaagat ttcctgactt accccctgac cttcgttccc catgattatt
     7861 gcgaagttat ctgatttttc tactttgtct aatgcgtgtg cttcttcatt taattctgtg
     7921 ccatacactg taaacccttt gtctttcatt aaagaaatca cttctgtcaa atctttttgc
     7981 aacacaggca aatgataatt gcttccctgc atgctgcgca gcacctttgt actataaata
     8041 tctgccgttc ctttgcctaa taaaacacca gccatatcaa atgcatctgc cgtacggatc
     8101 atcgtcccta catttccagg atcttgtaca ttatctaaca acagccatcc tcctgaaaaa
     8161 tctggcgtgc cttcagccgt taacgccatc actgctaaga ttccttgtgg tgtaggtaga
     8221 tccgatacag catccatgac ctcatccgaa actagaaaag tagaaatctc tttgtttgcg
     8281 gccagccagt ctccccattc tttcatgcct cgctgtgtca caaaaagttc ttttatttct
     8341 gcatcagacg acgcagcttc ttcaatcaga tggaaccctt ccaaaagata ttcttccttt
     8401 tcttcccgat atttcttttt atgtagtttt tttaattctt tgattcttgg attttttgct
     8461 gattggatct ctttcacatt cctcacctcc ccatgattat atcatggaat aggtgcttga
     8521 gcttgtttca tcctaaaaaa atcggtatca ttaatttata aaataggaaa gaaaggtgac
     8581 gaacatgcgc aaagttaaaa tgaacgtcca aggacgcgtc caaggcgtag gatttcgcta
     8641 catgaccaag atggtcgctg atcaactagg cgtgactgga acggtaaaaa acgaagatga
     8701 cggctcagtc acgatcgaag caatcggcaa tgacgacatc atccagaaat ttattgaaga
     8761 agtcaagaaa tctccgagcc caagtggacg agtgcagtat gtggatgtcc aagaagaccc
     8821 gatgattgag gaacgaaaga aatttgatgt ggtgggataa ggttgtgaaa aagctggtct
     8881 gcatcaagca ttaaggacag gttgtgaaaa atagctctcc atatttttca acaggctgga
     8941 cttaatgccg tagatgcaag cttttgaaca ccgtcgataa ggttattctt gaggcgcttt
     9001 gcctcaaaaa ttaaggacag cagtgaaaaa gctgatctat cacttgcagt gagttcattt
     9061 gactccgctt tttcgggcag aaaaaaagtt tttagtttat taaagtaaga aagaaacact
     9121 tggaatcatt ctactttttc ctagtatttc tttcttgatt ttttgtcttt ttttcaaaat
     9181 ttttcgctac gtaaaagaac gaatatttat atgaaatttc tatgaagttg attgaattcc
     9241 atgcgctttt ttagtatagt taagtttgtg taaaaattat agaagaggaa gtattctatg
     9301 aataaatgga aaaactggtt attgggatcc ggacttgtca gtatcctgct ctttttatct
     9361 ggctgtgtgc ggatggattc aaacggaaac cctgacactt cgggaatcgt ttatcgagta
     9421 ctcgtccatc ctatgggcca agcgattaca tatctagttg aaaatttcaa ttggtcttat
     9481 ggctgggcag ttattgcaat gactgtgatc gtccgtatca tcatcttgcc actaggtatt
     9541 agtcaatcga aaaaaacaat gatccaatct gaaaaaatgc aagcgttaaa accacaagtt
     9601 gaagcagccc aacaaaaatt gaaagctgca acaacacgtg aagaacaaat ggcagcacaa
     9661 gcagaaatgc agcaagtata ccgcgaaaac ggattgagta tgaccggcgg aatcggctgt
     9721 ctgcctctat tgatccaaat gccgatcttc tctgccttgt actttacggc acgttataca
     9781 gaaggtatcc gggaatcatc tttctacggc atcgatttag gctcaccaag tatggtactt
     9841 gttgccattg caggggtcgc ttatctgctt caaggataca tctcaacgat cggtattcct
     9901 gaagaacaga aaaagacgat gcgtacgatg ctgatcgtct ctcctgctat gatcgtcttc
     9961 atgtcgatca gtgcgccagc cggtgttaca ctttactggg tagtcggtgg tgtgttcagc
    10021 tgtctgcaaa cattcatcac aaacgttatg atgaaaccac gtttgaaagc acaagtggca
    10081 gaagagttga aacaaaaccc accaaaacaa gtcgtcactc caagaattga tgtgacagaa
    10141 tcagctaaac cagctgacag caaacaagca accaaacaaa tcaagcaacc caaaaacagt
    10201 acaaacaata aaggccgcaa cgcaggaaag cagcagaagc ggaagtagat agtaaataag
    10261 agtgtaataa aaattcatta acagttttag caattaaaaa tatcgaccaa gatttttgaa
    10321 aaagaaatct ggtcgatatt tttatttaag tgaatgttta atttgaatca tcgattgatc
    10381 gctatctaat tgcagcagca tctttttatg aagaaacaac tttttcttat ctatcaacgt
    10441 agacaggtgt tgctgtttct tctaatagtg tacttaattc cttaatattt tgttttcctt
    10501 cttctaacat ccaactactc gctttttctt cgccttcggt aacaaaaggc gccactccct
    10561 tcgcccatgt tgctcgccca cataagacac cattctccat aaaggacaac gcggagatac
    10621 cacccatcta aataacatcc ataaaccttt ctagtagaag aatagtgata gtatccatcc
    10681 ctttttctac tgggaataca attacacgat tcaaagcttg atacacaaca tttcccttct
    10741 tataaaccgc tattgtaacc gaattgtttt gaatcacctt tttgggatag aaactggctc
    10801 ctttccgcaa cttcttaaat gttggtttct tttatacttt ctttgtagca actacaaatt
    10861 tcttcaaatc tctaaatgtt tgcataacaa ccgcctttct gacttttact caggatggta
    10921 tttaaaaatc agcaaaataa ggcttctctc catctttttt atttcctagc tgctttaaag
    10981 acactcttgc tgttttttaa tctctatcaa gggggctctg aatctatttt gatgaactta
    11041 tcttattaat ttctcaaatg agctgtcaga cacaactgta ttttttcaat ctcataccgg
    11101 acaactatgc cagcttaaaa aaatttcctc tggcaaaact tctttattga aaaatgacaa
    11161 tgaaaaaaga ccattctttt tgttgaatgc tcattgtgta aacatatttc ctgcgactgc
    11221 tcttcgaacc attctcagtg caatccctaa tttatctatt aaatcttctt ttagttcctc
    11281 atctttcata ttcaaaattt cctttgtatg ttacacaagt ttgttctcta aataacgcga
    11341 attgtaaaat taagttagaa aaataaaaag gcatttatgg tacactcaaa ttgtatttcc
    11401 gacgaaagaa aacaaa
//
LOCUS       DACGLE010000082        11280 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0534
            SAMEA3304449-rid9560453.denovo.087, whole genome shotgun sequence.
ACCESSION   DACGLE010000082 DACGLE010000000
VERSION     DACGLE010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304449
            Sequence Read Archive: ERR985064
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11280)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11280)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702225.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702225.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 20:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,045
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 27 of 67
            Pseudo Genes (incomplete)         :: 25 of 67
            Pseudo Genes (internal stop)      :: 19 of 67
            Pseudo Genes (multiple problems)  :: 4 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11280
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304449-rid9560453.denovo.087"
                     /strain="VREN0534"
                     /db_xref="taxon:1352"
     gene            complement(56..565)
                     /locus_tag="IXT20_002346"
     CDS             complement(56..565)
                     /locus_tag="IXT20_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4860771.1"
                     /translation="MKPEYLIHAEHLTVILCPLEKQHKILEHLKKEVLVAKLDNFAII
                     QKNWPMFPYLNLKDHVLLDVPEKEIKQDRLAYQEKLDISPSLLNCAAAELTSFEKVKL
                     QLLHALLSKRNNIVIEDTFDELSVLEIQELLHLLSYLAHEENQGILLFTHDATIAQSP
                     YIDRLEPAG"
     gene            complement(555..1280)
                     /locus_tag="IXT20_002347"
     CDS             complement(555..1280)
                     /locus_tag="IXT20_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4860772.1"
                     /translation="MKIIRYATARLIYYKKQTILYCLFSSFSAFLLIACLTLYHLQSA
                     LANQVQDRLSFIGASASTELLPALELASRFYIKGIFSLFFLFAFLFIFFFYYSVRQNK
                     QELINWHLTGLSKTKLFLFIFWQLFFPLFLCCILVLLWIVSFQSVYEALLQRINFLVL
                     KTTELPDIRDILTSVSGLTIPMDQQTFFTIDFQNDLFFTDILKGFAQTLIGLSGCCLI
                     LSLFPFTFFIHHLQSRRPGRNET"
     gene            complement(1503..3623)
                     /locus_tag="IXT20_002348"
     CDS             complement(1503..3623)
                     /locus_tag="IXT20_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HAQ4860773.1"
                     /translation="MKCIKTPAFLNKRLGFFSLLVVLLWLKTIFAYLVDFHLGIESGI
                     QYFILFINPIATTLLLLGIALYIRRPRVAYITMMVIYFLLTLLVFSNVTYYREFTDFI
                     TINTILGAGKVASGLGESAIRLFRPYDVIYWIDFIILTVLLIAKKIKLDPRPIRARMA
                     FAISTLAVMIFSGNLFMAEADRPELLTRTFSRDYLVKYLGLNAFMVYDGVQTYQTNQV
                     RAEASPNDMKEVENYVKEHYAAPNDDLFGLARGKNVIYIHLESFQQFLIDYKLTDENG
                     LQHEVTPFINSLYHSNSTFSFDNFFHQVKAGKTSDAETLMENSLFGLNQGSLFTQLGG
                     KNTFQAAPDILSQTQGYISAVFHGNAGTFWNRNETYKNLGYDYFFDSSYYDVNDDNSF
                     QYGLHDKPFFQQSVQYLERLQQPFYSKFIAVSNHYPYSKFTNDEAGFPLANTNDETIN
                     GYFATANYLDTAVQEFFNYLKESGLYDNSVIVLYGDHYGVSNSRNKNLAELVGKTSST
                     WTNYDNAQMQRVPYMIHIPGQTQGGINHTYGGQVDALPTLLHLLGVDTKNYIQLGQDL
                     FSKDHEQLVSFRDGDFVTPKYTYYGGTIYSNETGEAITEPTEEVQNEIDSLKEKVDKQ
                     LAVSDQINNGDLLRFYTNSGLDPIDTSDYDYKNGLEKLKEAEKSLGDKSTSVFSEHNG
                     QSTVDLYKTQTYQELHGTTTSSSE"
     gene            3853..5031
                     /locus_tag="IXT20_002349"
     CDS             3853..5031
                     /locus_tag="IXT20_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HAQ4860774.1"
                     /translation="MKVKVKKRASNRLKKGYPLIQKEDLAHPQKETTQWVELVDSQNQ
                     FIAKGYLGKQNKGIGWVLSQQNIPFDQSFFEEFFVKSKEKRTFFFSDDSTTAFRIFNG
                     EGDGIGGLIVDYYAGYAVFSWYNETLYAHRELLIQAFREIYPEIIGGYEKIRFESVNL
                     PESQFIYGKEAPEPLIVKENGVKFAVYLNEGLMTGIFLDQKEVRGSLAEGFASGKTVL
                     NMFSYTGAFSVAAAMGGAIETTSVDLAKRSLKKTQEQFEVNGLDVDQQKIIVMDVFEY
                     FKYAKRKQFLYDMIVLDPPSFARNKKKVFRVAKDYGELVKDSLDILAEDGILIASTNA
                     ANVSIEQFQTMIEEEFHAKNVSFEQLALYRLPKDFQTTDTFLEGNYLKVFVYQIKNNH
                     "
     gene            5047..5805
                     /gene="aroD"
                     /locus_tag="IXT20_002350"
     CDS             5047..5805
                     /gene="aroD"
                     /locus_tag="IXT20_002350"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HAQ4860775.1"
                     /translation="MVRVKGLNIGSGRPKICAPLIGAVREEVLREAVLAREAGADLVE
                     WRVDHYREVFSFDEVAETLVLIHEMLDGLPLLFTFRTLAEGGEQDISIRQYRDLYEHV
                     IHTGLVDMVDIELFKVESLGKRLIEEIKSLNIPLIISSHDFKETPADPVLLYRLNVME
                     HFGADIGKLAVTPNNERDVLRLMELTRRANAFVSMPIITMSMGNLGKISRLVGGTTGS
                     VMTFGSLSSDSTSAPGQLPVKALKQIIQLLNEPL"
     gene            5828..6313
                     /gene="ybaK"
                     /locus_tag="IXT20_002351"
     CDS             5828..6313
                     /gene="ybaK"
                     /locus_tag="IXT20_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HAQ4860776.1"
                     /translation="MAKKKIVKTNAIRMVEQKKIPYTEHEYEWDESHLSASSVAEQLP
                     ESQSRIFKTLVAVGNVTGPLVAVIPGEAELNLKKLAKVSGNKKVEMLHLKDLEATTGY
                     IRGGCSPIGMKKLFPTYLDQIAESYEQIIVSAGRRGLQMELAPQDICALTSGQFADIK
                     Q"
     gene            complement(6384..7637)
                     /locus_tag="IXT20_002352"
     CDS             complement(6384..7637)
                     /locus_tag="IXT20_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAQ4860777.1"
                     /translation="MEKLYDVIVVGAGTSGMMAAISAAEQGARVLLVEKNKKAGKKLL
                     MTGGGRCNVTNNRPVDDLIAHIPGNGKFLYSTFAQWNNFDIMNFFESQGVHLKEEDHG
                     RMFPVTNKSKTIIEALLNRLKELDVTLLFSTRVEKLIHKEHKIYGIRTEFEEFHAPAI
                     ILTTGGRTYPSTGSTGDGYKIVKRVGHTVTPLYATESPLISDEPYIQEKTLQGLSLQD
                     ITLRVLNKKGRVLTEHTMDLLFTHFGISGPAALRCSSFVNKELEKTGEPVTLSLDCFP
                     TQTKQELIHILTEKSKATKKNLVNAWHGILPERLLVFFLERLEMDHLTGQQASQKQIQ
                     DFVQLCKEFKLSINKTFPIEKSFVTGGGVSLKEIYPKTLESKIIDGLYFAGELLDVNG
                     YTGGFNITAAFATGHVAGMNAGQRA"
     gene            7754..9103
                     /gene="gdhA"
                     /locus_tag="IXT20_002353"
     CDS             7754..9103
                     /gene="gdhA"
                     /locus_tag="IXT20_002353"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HAQ4860778.1"
                     /translation="MTNATEYVQAIQEKLHQKDQGQEEFLQAIDEFMPTVMSFLDTHP
                     EYIEKNILELLTEPERVIQFRVPWQDDEGNWRVNRGYRIQYNSAIGPYKGGLRFHPSV
                     NLSILKFLAFEQIFKNSLTGLPIGGGKGGSDFDPKGKSDNEIMRFCQSFMLELSKHIG
                     PSIDVPAGDIGVGAREIGYLFGEYKRLKKYDTGVLTGKPLDFWGSKIRTEATGYGLVY
                     YVKHLLNEEKDSFNQKTVFVSGSGNVAIYAIEKVQELGGKVVTCSDSSGYIYDPEGID
                     VKLLKEVKEINRQRLTEYANQRPSAIYHAGESVWTLKEKADIALPCATQNEIDESLAE
                     ILVENGIKIVAEGANMPCTVKAVSVLHEAGVWYCPGKAANAGGVAVSALEMSQNVQRL
                     TWEREQVDEQLDQIMETIYHTCRQTAAEYGDEKNLLLGANVAGFEKVAKAMAAEGLV"
     gene            9215..10561
                     /locus_tag="IXT20_002354"
     CDS             9215..10561
                     /locus_tag="IXT20_002354"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HAQ4860779.1"
                     /translation="MAHIHFDYSKVAPFVNEQELGYMQSQVTAAHNELREGTGAGSDF
                     RGWIDLPTNYDKEEFARIKEAAKKIQSDSEVLVVIGIGGSYLGARAAIDFLNHTFFNL
                     LDSNTRKAPQIFFAGNSISSTYIADLIEVIGDRDFSVNVISKSGTTTEPAIAFRVFKE
                     LLVKKYGQEEANKRIYATTDKAKGAVKVEADAEGWETFVIPDDIGGRFSVLTAVGLLP
                     IAVSGADIDALMQGAADASKAYSSDKLEENEAYQYAAMRNILYRKGKVTELLINYEPG
                     MQYFSEWWKQLYGESEGKDQKGIYPSSANFSTDLHSLGQFIQEGRRNIFETVVKVEKP
                     RKSITIPEQAEDLDGLGYLQGKEVDFVNTKAFEGTLLAHTDGDVPNLLVKIPEMDAYT
                     LGYTMYFFEIAVGISGYLNGINPFDQPGVEAYKKNMFALLGKPGFEDLAKELNERL"
     gene            complement(10869..11255)
                     /locus_tag="IXT20_002355"
     CDS             complement(10869..11255)
                     /locus_tag="IXT20_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HAQ4860780.1"
                     /translation="MKKILGLIVIILIAFIGWKGWDYYQSTYVGKDYYAVVKAPMPAE
                     TDIKADNGEVIGRGFKYNVDAYAENGEKRQLDFDVITSGDSANGSAYPEGTILQLKAS
                     EKRIIEKKVITSDKVPSSVKEKLGLS"
BASE COUNT         3670 a         2022 c         2293 g         3295 t
ORIGIN      
        1 aaaagatcat gaatcgatct tctataccgc ttgtttttac tataaaatga ttgatttagc
       61 ccgcgggctc taaacgatca atataaggac tttgagcaat agtagcatca tgagtaaaga
      121 gtaagatacc ttgattttct tcatgagcta aataagaaag caagtggagt agctcttgga
      181 tctccaatac actaagttca tcaaatgtat cttcaataac aatattgttt cgcttggaaa
      241 gcaaggcatg aagtaattgc agctttactt tttcaaaaga cgtcaactca gctgccgcac
      301 aattaagcaa ggaaggagag atatcaagtt tttcttgata ggccaaacga tcttgtttga
      361 tttctttttc cggtacatcc aacaggacat ggtctttaag attcagataa ggaaacatcg
      421 gccaattttt ttggatgatc gcaaaattat ccagttttgc aacaagaacc tcttttttca
      481 agtgctccaa tattttatgc tgtttttcga gaggacacaa aataacggtc aagtgttcag
      541 catgaatcaa atattcaggt ttcattacgt ccaggcctcc tactttgcaa atgatggata
      601 aaaaaagtga aagggaatag actaagaatg agacagcatc cactgagccc aataagagtt
      661 tgggcaaatc ccttaagtat atctgtgaaa aaaagatcat tttggaaatc gatggtaaaa
      721 aaagtctgtt gatccatcgg aattgtaagt ccgcttaccg aagtcaagat atcacggata
      781 tctggcagtt cagtagtctt taagacaaga aaattgatcc tttgaagaag cgcttcataa
      841 actgactgga agcttacgat ccacaataaa accaagatac aacaaagaaa gagaggaaag
      901 aacagctgcc agaaaataaa aaggaataat ttggttttag aaagaccagt caggtgccaa
      961 ttgatcaatt cctgcttgtt ttgcctgact gaatagtaaa agaaaaagat aaaaaggaag
     1021 gcaaatagaa aaaataaact gaatatgcct tttatataaa atcggctagc taattctaaa
     1081 gccggaagaa gctctgtaga tgcagaagcg ccaataaatg aaagccgatc ttggacttga
     1141 ttggctaaag ccgattgcaa atggtaaaga gtcaggcatg ctatcaataa aaaagcagaa
     1201 aagcttgaaa ataaacaata gagtattgtc tgctttttgt aataaattaa tcgagcagta
     1261 gcatagcgga ttattttcat acttttcacc tctataaaaa gtatagaaaa tgaatatgaa
     1321 taaagtatga aattccttta gtgaaatcac gaattaaaac cagaaaatat tttgtaaaaa
     1381 gcaagaggtt gtgaagaagc tgccctattc tattaccgta gatgcaagct tttgaacact
     1441 gtttattcga aaagaggagc agcgacaata attttgtcgc tgctcctttt aaatttattt
     1501 tatcattcgg aagaacttgt ggtcgttccg tgaagttctt ggtacgtttg cgttttataa
     1561 agatcaacag ttgattgtcc gttgtgttca ctaaagacac tagttgactt gtcaccaaga
     1621 gatttttcag cttcttttaa tttctctaag ccattcttgt agtcataatc actggtatca
     1681 attgggtcta atccactatt cgtatagaac cgaagaagat caccattatt gatttggtca
     1741 gaaacagcta actgtttatc aactttttct ttcagtgaat caatttcatt ctgtacttcc
     1801 tcagtaggtt cagtaatagc ttcaccagtt tcgttactat agatcgtacc accgtagtaa
     1861 gtatattttg gtgtaacaaa atcaccatca cggaaagaaa caagctgttc atgatctttc
     1921 gagaatagat cttgtcctaa ctgaatataa ttttttgtat ctacacctag taagtgaagt
     1981 aatgtaggca aggcatcaac ttgtccgccg taagtgtgat tgattccgcc ctgtgtttgt
     2041 ccagggatat ggatcatata agggacacgc tgcatttgtg cattgtcgta gtttgtccaa
     2101 gtagaacttg tttttccaac taattcagct agatttttat ttcttgaatt cgagacaccg
     2161 taatggtcac cgtaaaggac gatgactgaa ttatcataca agccgctttc tttcaaataa
     2221 ttgaagaatt cttgtacggc ggtatctaaa taattggctg tcgcaaagta accgttgatc
     2281 gtttcatcat ttgtattggc taacgggaat cctgcttcgt cattggtgaa ttttgaataa
     2341 ggataatggt tagatacagc aatgaatttt gaataaaatg gttgttgcag acgttccaag
     2401 tattgtacgg attgttggaa aaatggttta tcatgcaacc cgtattggaa cgaattgtca
     2461 tcattaacat cataataaga agaatcaaag aaataatcat atcctaaatt cttgtatgtc
     2521 tcgtttcgat tccagaatgt accagcgttc ccatggaata ccgcactaat gtaaccttgt
     2581 gtttgactta aaatatcagg tgcagcttgg aaagtatttt ttccgcctaa ttgtgtgaac
     2641 aaggagcctt gattcaagcc aaacaatgaa ttttccatca gcgtttccgc atcgcttgtt
     2701 tttcctgctt ttacttgatg gaaaaagttg tcaaaactga acgtgctgtt gctgtggtaa
     2761 agactattga tgaaaggtgt aacttcatgc tggagaccat tctcatctgt caatttataa
     2821 tcgatcagga attgctggaa gctttctaaa tggatataaa tcacattttt tcctctagcg
     2881 agcccgaaca aatcgtcatt tggtgctgca taatgttctt ttacataatt ttctacttct
     2941 ttcatatcat tgggacttgc ttctgcccgc acttggtttg tctggtaagt ttgaacaccg
     3001 tcataaacca tgaaagcatt caagccaaga tatttgacca aataatcacg agagaatgtt
     3061 cgagtcagca attctggacg atcagcttcc gccataaaaa gatttcctga gaaaatcata
     3121 actgctaatg tagagatcgc aaaagccatt cttgctcgga tcgggcgagg gtccaacttg
     3181 attttttttg caatcagcag aacagtcaag ataataaaat cgatccaata gatcacatca
     3241 tatgggcgaa acagtcgaat ggcactttct cccagaccac tcgctacttt cccagcacct
     3301 aaaatcgtgt tgatcgtaat gaagtccgtg aattctcgat aataagtgac attcgaaaaa
     3361 acaagcaatg tcaaaagaaa ataaatgacc atcatcgtga tataagcaac tctagggcgt
     3421 ctgatataaa gagcaattcc aagtaataac aatgtagttg caatagggtt gataaacaag
     3481 ataaagtatt gaatgccact ttcgattccg agatggaaat caactaaata ggcaaaaatc
     3541 gttttcaacc aaagtaaaac gactagtaaa gaaaaaaatc cgagcctctt attcaaaaag
     3601 gcaggtgttt ttatacattt caagataatc ttcctttctg cacttcttaa tcacagaagt
     3661 gtcctgaaac catgttacac agctcactta ttctcattta ttttactagt atgacaatat
     3721 agtgtcaatt gaaaccagaa gtttatgctg tttcattaag gttttcttta agcttcatga
     3781 aaaaatgagc agtaagacaa gtcctatgtt ttttgttata cttgggaaga gaaagctaaa
     3841 gcaggtgaag gaatgaaagt aaaagtaaaa aaaagagcaa gcaatcgatt gaaaaaagga
     3901 tatccactga tccaaaaaga agaccttgct catccccaaa aagaaaccac gcaatgggtg
     3961 gaattagttg attcgcaaaa ccaatttatc gcaaaaggat atctaggaaa gcagaataag
     4021 ggaatcggtt gggtattgag ccagcagaac atcccatttg atcaatcttt ttttgaagaa
     4081 ttttttgtga agtcaaaaga aaaaagaact ttcttttttt cagatgattc tactaccgct
     4141 tttcgtatat tcaatggaga aggagacggg atcggcggtc tgattgttga ttattatgca
     4201 ggttatgctg ttttttcttg gtataacgaa acgttatatg cacatcgtga attattgatt
     4261 caagcttttc gtgaaatcta cccggaaatc ataggaggct atgagaaaat tcgatttgaa
     4321 tcagtaaatc tgcctgagag ccagttcatt tacggaaaag aggcaccgga acccttgatc
     4381 gtaaaagaaa acggagtaaa atttgccgtt tatttaaatg aaggattaat gactgggatc
     4441 tttttagacc aaaaagaagt acggggatca ttagcagaag gtttcgcttc tgggaaaaca
     4501 gttttgaaca tgtttagtta taccggcgcc ttttctgttg ctgccgcaat gggaggggca
     4561 atcgaaacaa caagtgtcga tctagcaaaa cgcagtttaa aaaaaacaca ggaacagttt
     4621 gaagtgaatg gtttggatgt cgatcagcaa aaaatcattg ttatggacgt attcgagtat
     4681 ttcaaatacg ccaaaagaaa acaattttta tatgacatga ttgtattgga tccaccaagt
     4741 tttgccagaa ataagaaaaa agtctttcgc gtagcaaaag attatgggga actggtcaag
     4801 gatagtctgg atattttagc agaagacggg atacttattg cgtctacaaa tgctgcgaat
     4861 gtctcgatag aacaattcca aacaatgatt gaagaggaat tccatgccaa aaatgtttct
     4921 tttgaacaac ttgctttata tcgtctgcca aaagatttcc agacgacgga taccttttta
     4981 gaaggcaatt atctgaaagt gtttgtttat caaattaaaa ataaccatta gaaggagcga
     5041 tgcagaatgg ttcgggtaaa gggattgaat ataggaagcg gtagaccaaa gatttgtgcg
     5101 cctcttatcg gggccgtccg agaggaagta ttacgtgaag cagttcttgc acgtgaagca
     5161 ggggccgatt tagtcgaatg gcgagtcgat cattatcgtg aagttttttc ttttgatgaa
     5221 gtagcagaaa cattggttct tatccatgag atgcttgatg gactgccgtt attatttacg
     5281 tttcgtacct tagctgaagg cggagaacaa gacatatcga tccgtcagta tcgcgacctt
     5341 tatgaacatg tgatacacac aggattagta gatatggtcg atatcgagtt gttcaaagta
     5401 gaaagtctag gaaaaagact gatagaggaa atcaagtctt taaacattcc tttaattatc
     5461 agtagtcatg attttaaaga aacaccagca gaccctgtct tgctttaccg attgaatgtt
     5521 atggaacatt ttggagccga tatcggaaaa ctggcagtaa ctcccaataa tgaacgagat
     5581 gttttgcggt tgatggagtt gactagacgg gcaaatgctt ttgtctccat gcctattatt
     5641 acgatgtcca tgggaaatct tggtaaaatt tctcgtctgg ttggcggaac gacgggttcc
     5701 gttatgacat ttggttcctt aagcagcgat tctacttctg ctccaggaca attaccggtc
     5761 aaagcattga agcaaattat tcaattatta aatgaaccgt tgtagaacaa gtttgaggtg
     5821 atattttttg gcaaagaaaa aaatagtaaa aacgaatgca atccgtatgg tcgaacaaaa
     5881 aaagattccc tatacagagc acgaatatga gtgggacgaa agccacctaa gtgcgtcatc
     5941 ggtcgctgaa cagcttccag aaagccaatc tcgtatattt aaaacactgg ttgctgtggg
     6001 caatgtgacg gggcctcttg ttgctgtgat accgggagaa gccgaattga atctaaaaaa
     6061 attagcaaaa gtttcaggga ataaaaaagt agaaatgctc catttaaaag atttggaagc
     6121 aacgaccgga tacatacgtg gtggctgctc gcctataggt atgaaaaaac tatttcctac
     6181 ttatttagat caaattgcag aaagctatga acaaatcatt gtctcggctg gcagaagagg
     6241 gctacagatg gagttagcac ctcaagatat ctgtgcatta acgagcggac aatttgcaga
     6301 tataaaacaa taaatcatac gtataaaaga aagaaggagg ccgggatgtt gtttgtccag
     6361 tctccttctt tcttttttat acattatgct ctttgcccgg cattcattcc tgctacatgc
     6421 cctgtagcga aagccgctgt gatattaaag ccgccagtat aaccattgac atccagtaat
     6481 tctcccgcaa aatacagacc atcaatgatt ttactctcca atgttttagg ataaatttcc
     6541 tttagagaaa cccctccgcc agttacaaat gatttctcaa taggaaaagt tttgttgatt
     6601 gatagcttaa actctttgca tagttgaaca aaatcttgaa tttgtttttg agaggcttgt
     6661 tgtcctgtca gatgatccat ttcaagccgc tcaagaaaga aaactaacaa tcgctcaggc
     6721 aatataccat gccaagcatt gaccaagttc tttttggttg ctttactttt ttctgtcaat
     6781 atatgaataa gctcttgttt ggtttgtgta gggaaacaat ccagagaaag tgtcactggt
     6841 tctcccgttt tctctaattc tttattgaca aaacttgaac aacgcagtgc agccggacca
     6901 gaaataccga aatgggtgaa aagtaaatcc atcgtatgtt cagtcaatac acgacctttt
     6961 ttatttaaaa cgcgtaaagt gatgtcttgt aaagaaagcc cttgaagtgt tttctcctga
     7021 atatacggtt catccgatat caagggggac tctgtcgcat aaagaggcgt caccgtgtga
     7081 cccactcgct tcacgatctt gtacccatct ccagtagaac cagtagatgg ataagtccgt
     7141 ccaccagttg ttaagataat ggcaggagca tggaactctt cgaattccgt gcgtataccg
     7201 taaattttat gttctttgtg gatcaatttc tctactcgag tagagaatag aagcgtcaca
     7261 tctagttctt tcagacgatt gagcaatgct tcgataatcg tttttgattt atttgttaca
     7321 ggaaacatcc gtccatgatc ctcttctttt aaatgtacgc cttgtgattc aaagaagttc
     7381 atgatatcga aattattcca ttgcgcaaac gtactatata aaaactttcc atttccagga
     7441 atatgtgcaa tcaaatcatc aacaggacga ttgttcgtaa cattgcaccg accaccacct
     7501 gtcatcagta gtttttttcc tgcttttttg tttttttcga ccaagagtac gcgtgcgcct
     7561 tgttcagctg cactgatcgc tgccatcatc ccgcttgttc cagcgccgac tacgatcaca
     7621 tcatacagtt tttccattcc ctcacctcaa ggaaagtgta gcatatctga agaaagaaaa
     7681 cagaaaaaaa tgttttgagc caaaagagaa aatgagctaa aatgagggaa gataagcaaa
     7741 ggagcgtgga acgatgacaa acgcaacaga atatgttcaa gcaattcaag aaaagctaca
     7801 tcaaaaggat caagggcaag aagaattttt acaagcaatc gatgaattca tgcccacggt
     7861 tatgtctttt ttggacactc atcccgaata tatcgaaaag aatatcttag aacttttaac
     7921 ggaaccagaa agagttattc aatttcgagt gccttggcaa gatgatgaag gaaactggcg
     7981 agtaaataga gggtaccgta tccaatacaa ctcagctatc ggcccttata aaggtggatt
     8041 gcgcttccat cctagtgtca atctaagtat cttgaaattt ttggcttttg aacagatttt
     8101 caagaatagt ttaaccggtc tgccaatcgg tggaggaaaa ggcggaagtg acttcgatcc
     8161 taaaggaaaa tctgataatg agatcatgcg tttttgtcaa agtttcatgc tggaattgtc
     8221 aaaacatatc ggcccatcga ttgatgttcc tgcgggagat atcggtgtag gagctagaga
     8281 aatcggctat ttgttcggcg aatataaacg attgaaaaaa tatgatactg gtgtgttgac
     8341 aggtaaacct ttggattttt ggggaagtaa gatacgtacg gaagctactg gttatggatt
     8401 agtgtattat gtcaaacatc tattgaacga agaaaaagat tcatttaatc aaaaaacagt
     8461 ttttgtttcc ggaagcggga atgtagccat ttatgcaatc gaaaaagtgc aagagctagg
     8521 cgggaaagtt gttacatgtt cagattctag cgggtacatc tatgatccag aaggaataga
     8581 tgtgaaacta ttgaaagaag tcaaagaaat caatagacaa cgtttgacgg aatacgcaaa
     8641 ccaacgtcct tctgctattt atcatgctgg agaatctgtt tggacattga aagaaaaagc
     8701 ggatatcgct ttgccatgtg ctactcaaaa tgaaatagac gaaagcttgg ccgaaatctt
     8761 agtagaaaat gggatcaaaa tcgtagcaga aggcgctaat atgccatgta cagtaaaagc
     8821 tgtttctgtt cttcatgaag caggcgtgtg gtattgtcca ggaaaagctg ctaatgctgg
     8881 aggagtagca gtatctgctt tagaaatgag tcagaacgtc cagcgattga cttgggaaag
     8941 agagcaagtg gatgagcagc tggatcaaat catggaaacg atttaccata cgtgtcgcca
     9001 aacagcagcc gagtacggcg acgaaaaaaa tctgcttctt ggagcaaatg ttgctggatt
     9061 tgaaaaagtt gccaaagcaa tggcagcaga aggtttagtt tgaaaaatga aactttcttg
     9121 gaaatattgt cagcattttg tttatttgct tgaaaaataa taaaaaaaaa cgtaaagtat
     9181 tacctgtaga gtaaacctat aaggaggaga acaaatggca cacattcatt ttgactattc
     9241 aaaagtagca ccgtttgtca acgaacaaga gttagggtac atgcaaagcc aagtaacagc
     9301 agcccacaac gaattacggg aaggtactgg cgcaggaagc gacttccgcg gctggatcga
     9361 tttaccaacg aactatgaca aagaagaatt tgcacgtatc aaagaagcag ctaaaaaaat
     9421 ccaatctgat tctgaagtat tagttgttat cggtatcggt ggttcttatt taggcgctcg
     9481 tgcagcgatc gacttcttga atcacacatt cttcaattta ttagatagca acacaagaaa
     9541 agcaccacaa atcttctttg ccggaaattc aatcagctca acatatattg ctgacttgat
     9601 cgaagtaatc ggtgatcgcg acttttcagt aaacgttatt tcaaaatctg gaacaactac
     9661 tgaaccagcg attgcgttcc gtgtattcaa agagcttctt gttaagaaat atggacaaga
     9721 agaagcaaac aaacgtatct acgctacaac agataaagca aaaggtgcag ttaaagtaga
     9781 agcagatgca gaaggttggg aaactttcgt gattcctgat gacatcggcg gacgtttctc
     9841 tgtattaact gctgttggtt tgttaccaat cgctgttagt ggtgcagata tcgatgcatt
     9901 gatgcaaggt gccgctgatg caagcaaagc atactcaagc gacaaactag aagaaaacga
     9961 agcttaccaa tacgcagcaa tgcgcaacat tctttaccgt aaaggcaaag taactgagtt
    10021 gttgatcaac tatgaaccag gaatgcaata cttctcagaa tggtggaaac aattgtatgg
    10081 tgaatctgaa ggaaaagacc aaaaaggcat ctatccttca agcgcaaact tctcaactga
    10141 tttgcactct ttaggacaat tcatccaaga aggacgtcgc aatatttttg aaacagttgt
    10201 gaaggttgaa aaacctcgta aatcaatcac aattcctgaa caagcagaag atttagacgg
    10261 tctaggctac ttgcaaggaa aagaagtgga cttcgtaaat acaaaagcat ttgaaggaac
    10321 attgcttgct catacagacg gtgacgtgcc taacttgcta gtcaaaattc cagaaatgga
    10381 tgcttataca ttgggttata caatgtactt ctttgaaatt gctgtaggta tttcaggtta
    10441 cttgaatgga atcaacccat tcgaccaacc aggtgtagaa gcttacaaga aaaacatgtt
    10501 cgcacttcta ggcaaaccag gctttgaaga tcttgctaaa gaattgaacg aacgcctgta
    10561 agataagggt ttttcgctag atttaaataa tcagtgatag aaacatgttc gctttaaaat
    10621 gaaaactggt acccatttag gtactcaaac atactgcatc gtttattcag aataaggggc
    10681 tgtgacaaga cgtttgtcac agtcccttgt ttgtatagaa atcgattgat tgccttgctc
    10741 tttagtttgt tttaggactt taagaacagt gaggctgact tcgaaggtta attctgtttt
    10801 tttgttttct agaatcagta agcaaaaaag cactcacttc gagtgctttt ttgcgatata
    10861 ttcttagctt aagataggcc taatttttct tttacagatg agggtacctt atcgctggta
    10921 attacttttt tctctatgat ccgcttttcg cttgctttca actgcaagat cgttccttcg
    10981 gggtaagcag aaccattggc agagtcacca gaagtaatga catcgaaatc taactgtctt
    11041 ttttcaccgt tttcagcata agcatctacg ttgtatttaa aacctcttcc aattacttcc
    11101 ccattatctg ctttgatatc agtttctgca ggcattggag ctttgactac cgcataataa
    11161 tctttaccta cataagtaga ctgatagtag tcccaacctt tccatcctat aaaagcaatc
    11221 aatatgataa cgatcaaccc taaaattttt ttcatgaaag atctcctttt ttatttttat
//
LOCUS       DACGLE010000083        11265 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0534
            SAMEA3304449-rid9560453.denovo.088, whole genome shotgun sequence.
ACCESSION   DACGLE010000083 DACGLE010000000
VERSION     DACGLE010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304449
            Sequence Read Archive: ERR985064
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11265)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11265)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702225.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702225.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 20:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,045
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 27 of 67
            Pseudo Genes (incomplete)         :: 25 of 67
            Pseudo Genes (internal stop)      :: 19 of 67
            Pseudo Genes (multiple problems)  :: 4 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11265
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304449-rid9560453.denovo.088"
                     /strain="VREN0534"
                     /db_xref="taxon:1352"
     gene            <1..620
                     /locus_tag="IXT20_002356"
     CDS             <1..620
                     /locus_tag="IXT20_002356"
                     /inference="COORDINATES: protein motif:HMM:TIGR00350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAQ4860781.1"
                     /translation="VDIIGYDTYDKINHAYAFGGVAMTINTIQAYLDIPIDFFVTLNM
                     QGLEQIVDSIGGITLTPTATFTQDSYSFYQGEIINLDGKASLAYSRNRHADDSGDYGR
                     QARQREIIMAIIEKGTKVTTLATKIPSLIKSLQGNMTTNLSVDDMWTIINKYSTALGN
                     IEQVQLKGEGQLLDDGIYYEMINEESLEEVQNILQKQLDYHLNID"
     gene            670..1194
                     /locus_tag="IXT20_002357"
     CDS             670..1194
                     /locus_tag="IXT20_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007722706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ4860782.1"
                     /translation="MQNDIRGKIKKSRHIKGISQSEVAKYLNISRQSISKWENDTSSP
                     DINNLTLLSSLYEISIDDLLKEKKEVKKNIKENSIEIIESKNKLIYLKNNTNRDEGLV
                     LLLISLIGCLISPLGLIIAPVVLKRNKATNTFYKGVIAASVICFTVSLFVSYGHFSNI
                     FHWGGSTNVEYIEN"
     gene            complement(1191..1655)
                     /locus_tag="IXT20_002358"
     CDS             complement(1191..1655)
                     /locus_tag="IXT20_002358"
                     /inference="COORDINATES: protein motif:HMM:NF037267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4860783.1"
                     /translation="MRQVLLVVTLCLSFFLFEVNTVEAYVANNGVYYNLIQGGTQEFM
                     EVDEEGNPILIRVEEVPGLSLFSVNNGNYKISGSKFGMWKANYYVTVSNNQFIRAYSP
                     SAVAVTGSFTSTRLSLVSSKKASYTLEWKAGILSSNSYLDATLTDNSINITY"
     gene            2569..4110
                     /locus_tag="IXT20_002359"
     CDS             2569..4110
                     /locus_tag="IXT20_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010632830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HAQ4860784.1"
                     /translation="MFRYRRILEMHSEGISIRSIAASTGNSRQKITEVIRKAEEKGIA
                     RALSEEMTDQWLGEFLFPEKQLEANGRHLPDFDKIHKELAKPNVTLTLLHQEYAVECR
                     MSGKIPYAYRTFCDYYNAFARKHKATMRIRRKPGEIMEVDWAGSPAYVIDRDTGEKVK
                     AYVFVATLPCSQLSYAEAFLTMKSVAWITAHNHAFEYFGGSTDIIVPDNLKTGVKKNE
                     GIDVILNESYRELGEHYGSIILPARVSTPKDKPSVEGSVGVISTWIIAALRNTHCFTI
                     EDLNREIRMKLNEFNERPFTKKPGSRIRAFEEEEKFALNPLPLAPYKMSVWKKAKVQL
                     NYHVSVESMFYSVPYEYIQQNIDIRMTENLIELYFNSMRIATHKRLYGKFGQYSTVRD
                     HMPDNHKMYVDHTPESSMEWAETIGSSTVKVVQHLLDRYQNEKQALKSIMSLKSFGKK
                     YSKNEIETACSTALNLASLPSVKIIQTILKGNKQKRATSQSHIEDTNIEKHGFTRGAS
                     YFGGK"
     gene            4110..4862
                     /locus_tag="IXT20_002360"
     CDS             4110..4862
                     /locus_tag="IXT20_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAQ4860785.1"
                     /translation="MMLNEQTEVKLAELKLSGMLESYREQMTNKEYQSMSFEDRFNLM
                     VDLEHSRRKNNKLQRLIKSANFRNASASIEDIEYHPDRNLDKNLILKLASGTYLEEHH
                     NIILMGASGNGKTYIANAFGIQACRQYYNVKYIRLPELLDELTISKNQADGSFRKIIN
                     RYKKVQLLIIDEWLLTNLSLENSTFLLEIIESRLKTASTIFCSQFHPEGWYEKLGNPQ
                     IADAILDRIIHDSYQILVDGKVSMRERYGIRN"
     gene            complement(5045..5620)
                     /locus_tag="IXT20_002361"
     CDS             complement(5045..5620)
                     /locus_tag="IXT20_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4860786.1"
                     /translation="MKNKFKYLFTILALLPITTSTLITPTIAKASEKQEDSVSYEIVK
                     NDLYIQFEEELAELIASNQSTVSVPDLFSTQSVLSTSSQDNSISEEQFFSLVEKYDGA
                     ELTAVVNSQDAFTTKFVNGGVNSYATSYRGLNALRSELKYNAGALKVGGTLVGTAGGL
                     LGVLLGMVTGDYLSGHCDDAAAGVSAFIQAG"
     gene            6408..6980
                     /locus_tag="IXT20_002362"
     CDS             6408..6980
                     /locus_tag="IXT20_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIIA family hydrolase"
                     /protein_id="HAQ4860787.1"
                     /translation="MNKAIFWDLLGTLGGDSRTLINQNFNFFKDTIPALKKAAQSHYL
                     NIIITNQSHISHGRITMAEYTDALESLINELEKNDIQITDVYTCPHARKDNCDCKKPQ
                     PTFVNEALKKYDLNPSKCFVVGDSGKNDMMLAKNAGINSVLLLTGEGIDSLTKYRHLW
                     SETKPTFIAENSLDAINKIHMYADGDSSDS"
     gene            7173..7736
                     /locus_tag="IXT20_002363"
     CDS             7173..7736
                     /locus_tag="IXT20_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000017403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAQ4860788.1"
                     /translation="MSGYIYGYARVSTRSQELNRQLDLLNEQNCNEILTEKMTGTKAV
                     RPQLNRLKDKIRPGDTVIVESFSRLGRSTKDLIDLVTYFEEHHVKLVSLKENFDTSTP
                     HGRLMMTVFQAFSQFERDLIVERTKEGLKSARARGRKGGRPRVNQKDVDRAVKLYKSQ
                     VYSVKEITEMTGISKATLYRYLKDNRE"
     gene            complement(8015..8302)
                     /locus_tag="IXT20_002364"
     CDS             complement(8015..8302)
                     /locus_tag="IXT20_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000312842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HAQ4860789.1"
                     /translation="MAYEILPSKHVIKYLKKLKEKTLKEQFLTIIYDEIAVRPHSGEQ
                     KTGDLSGIWAIGFKYAGTTYRVAYEIKDNAVIPILLCGTHENFYEQLKKIR"
     gene            complement(8292..8621)
                     /locus_tag="IXT20_002365"
     CDS             complement(8292..8621)
                     /locus_tag="IXT20_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007723369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAQ4860790.1"
                     /translation="MITKTRKQGNSIMLTVPKEFDVPNGVEVEAKLVENGILYEFVEP
                     KKEFFDFSEDILADILSEGYSKQEILKEFRNRKNALTSAFKSIAEDTVANSKPMTKEE
                     LAAEIGL"
     gene            8926..9255
                     /locus_tag="IXT20_002366"
     CDS             8926..9255
                     /locus_tag="IXT20_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000754864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ4860791.1"
                     /translation="MKKVCQVTVVHKEKVNGIKNKLDQKDFSNLLVLGKCFSDFSRIK
                     IFYALETYKEMCVCDLAEILTASVATTSHHLRFLKKHGMAKSRQDGKVVYYSLANEDI
                     LSAIRVF"
     gene            complement(9542..10027)
                     /gene="vanZ"
                     /locus_tag="IXT20_002367"
     CDS             complement(9542..10027)
                     /gene="vanZ"
                     /locus_tag="IXT20_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000516404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycopeptide resistance protein VanZ-A"
                     /protein_id="HAQ4860792.1"
                     /translation="MGKILSRGLLALYLVTLIWLVLFKLQYNILSVFNYHQRSLNLTP
                     FTATGNFREMIDNVIIFIPFGLLLNVNFKEIGFLPKFAFVLVLSLTFEIIQFIFAIGA
                     TDITDVITNTVGGFLGLKLYGLSNKHMNQKKLDRVIIFVGILLLVLLLVYRTHLRINY
                     V"
     gene            complement(10180..11091)
                     /locus_tag="IXT20_002368"
     CDS             complement(10180..11091)
                     /locus_tag="IXT20_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala carboxypeptidase VanY-A"
                     /protein_id="HAQ4860793.1"
                     /translation="MKKLFFLLLLLFLIYLGYDYVNEALFSQEKVEFQNYDQNPKEHL
                     ENSGTSENTQEKTITEEQVYQGNLLLINSKYPVRQESVKSDIVNLSKHDELINGYGLL
                     DSNIYMSKEIAQKFSEMVNDAVKGGVSHFIINSGYRDFDEQSVLYQEMGAEYALPAGY
                     SEHNSGLSLDVGSSLTKMERAPEGKWIEENAWKYGFILRYPEDKTELTGIQYEPWHIR
                     YVGLPHSAIMKEKNFVLEEYMDYLKEEKTISVSVNGEKYEIFYYPVTKNTTIHVPTNL
                     RYEISGNNIDGVIVTVFPGSTHTNSRR"
BASE COUNT         3824 a         1973 c         1873 g         3595 t
ORIGIN      
        1 atgtcgatat tatcggttat gatacttacg acaaaattaa ccatgcctat gcctttggtg
       61 gagtagccat gactataaat acaatacaag catacttaga tatacctatt gacttttttg
      121 taacgttaaa tatgcagggt ctagaacaaa ttgttgattc tattggagga ataactctta
      181 ctccaactgc cacttttaca caagatagtt attcatttta tcaaggcgaa attattaact
      241 tagatgggaa ggcttcctta gcgtactctc gaaatcgtca tgcagatgat tcaggtgact
      301 atgggagaca agctcgtcaa agggaaataa taatggcaat tatagagaaa ggaactaaag
      361 ttactacact agcaactaag ataccttcac tcattaagag tcttcaggga aatatgacta
      421 ctaatctgtc agttgacgat atgtggacta ttattaataa atattccact gctttaggaa
      481 atatagagca agttcaatta aagggggaag gacaactact tgatgacgga atatactatg
      541 aaatgattaa cgaagaatct ttagaagaag ttcagaatat ccttcaaaaa caactagatt
      601 atcacttgaa tatagactaa ataatctatt tcttaatatc caataaataa aagtcattaa
      661 taggagagga tacagaatga cattagggga aaaattaaaa aatcacggca tataaaaggc
      721 atctctcaaa gtgaagtagc aaaatattta aatatatcta gacaatctat ttctaaatgg
      781 gaaaatgata ctagttcccc agatataaac aacttaactt tattaagtag tttatatgaa
      841 atctctatcg atgatctttt aaaagaaaaa aaagaagtta aaaaaaatat aaaagagaat
      901 agtatagaga taatagaatc taaaaataaa ttaatttatt taaaaaataa tacaaaccga
      961 gatgaagggt tagttttatt gctcatttcc ttaatcggct gtttgatttc tcctcttgga
     1021 ctaattattg cgcctgttgt tctgaaaaga aataaagcaa caaacacttt ttataaagga
     1081 gtgattgctg cttcagtcat ctgttttaca gtaagcctgt ttgtctccta tggacatttt
     1141 agtaatatct ttcattgggg cggctccact aacgtggaat atattgagaa ctaatacgta
     1201 atattaatgg aattatctgt cagtgtagca tctagataag agttactaga taaaattcca
     1261 gctttccatt ctaatgtgta gctagctttt ttgctggaga cgagagacag tctcgtgcta
     1321 gtaaaggatc cggtcactgc gactgcagaa ggggagtagg ccctaataaa ttggttattc
     1381 gagactgtca catagtaatt tgccttccac attccaaatt tagatccaga tattttataa
     1441 tttccgttgt tgacggagaa cagggagagc ccgggaacct cttccacacg aatcaagata
     1501 ggatttcctt cttcgtctac ctccatgaac tcttgcgtgc caccttggat taagttatag
     1561 tagactccat tatttgccac atatgcttct acagtgttta cttcaaataa aaagaaagat
     1621 aaacataaag taacaaccaa taaaacttgt ctcaaaaaat ccctcctaaa attaatatac
     1681 tcatagttta atatatacgg gtttaattag taagagaaca ttttgtgaac attcttatat
     1741 tgaaacggct ctctctacaa atggacgcat atataagtgt atctgggtta gtaaacattc
     1801 actgttacca actctactta ctaaacagat aaaatatcaa cgaaataaaa aaaccaaatc
     1861 aactaatttt ttactaataa cgtagataat aaaatcaaaa agcaaatttg agcggttgca
     1921 actataaagt gacctgatgt aaaccataag atgcttaatt aagctatact tagatgagac
     1981 actatactta ggaagaactt agattagcat agcttcctag aacaaaaaaa tacgagagga
     2041 agattctttt atgactaata aatatctatc tgtatttact tttttggttg gcttgttttt
     2101 tgttattaat agtgtagcaa gttctgattt tgatcttagt aagattactc ttttaactgt
     2161 actaccaata ataataggta gtgtgttaat tcttttttct gtgacttccc tttttagaca
     2221 gcgaggtaat tccaaaaagc attagcgaaa aagataaaag cagttttctc tttacccaag
     2281 agagaactgc tttttatcat ctccacattt cttgcttaat taattacagc ttcataaata
     2341 ctatcaatat ggctaatagg gaaaccttca gtaagggtta ccctagatcc tcctctagtg
     2401 taaccagtga ttacggcatc gaaaatgtac catctctgca ataattttta ccaggctcgg
     2461 caaagaaatg taccagtaag tgcaaagcag tgtaccacaa atacctcttg tataatgtag
     2521 gtgcacacct tagggtgtga catttatttt acaggaggta tttttattat gtttcgttat
     2581 cgaaggatac ttgaaatgca cagtgaagga attagtattc gcagtattgc agcaagcacg
     2641 gggaactcac gtcagaaaat tactgaggtt atccgaaaag ctgaggagaa agggatagcg
     2701 agggcgttgt cggaagaaat gaccgatcaa tggctaggcg aatttttgtt tccagaaaaa
     2761 caattagaag ccaatggacg gcacctgcct gacttcgaca agattcacaa ggaattggcc
     2821 aaaccaaacg tgaccttgac gcttcttcat caggaatacg cagttgagtg tcgcatgagt
     2881 gggaaaatcc cttacgccta tcgtacattt tgtgactatt acaatgcgtt cgcgcgaaag
     2941 cataaagcca ctatgcgcat ccgtcggaag cctggtgaaa ttatggaagt ggattgggct
     3001 ggatcgccag cttatgtcat cgatcgagat acgggtgaaa aagtaaaggc ttatgtcttt
     3061 gtggcaacct taccgtgcag ccaattgtct tatgcggaag cattcctaac gatgaaatcg
     3121 gtggcttgga ttacggcaca taaccatgca tttgaatatt ttggcggttc taccgacata
     3181 attgttcccg ataatctaaa aacaggtgta aaaaagaatg aaggcatcga tgtcatcctt
     3241 aatgaatcat atcgagagct aggggaacat tacgggtcca tcattttacc tgcccgagtt
     3301 tctactccca aggataagcc cagtgttgaa ggcagcgtag gcgtcatctc cacttggatt
     3361 atagctgcct tacgcaatac gcattgtttt acaatcgaag atttaaatcg tgaaatcaga
     3421 atgaaattaa atgaattcaa tgagcgaccg ttcactaaga aaccaggatc caggatcaga
     3481 gccttcgagg aagaagaaaa attcgcactt aatcctcttc cactggcacc ttataagatg
     3541 tctgtctgga aaaaagcaaa ggttcaacta aactaccacg tctcagttga aagcatgttt
     3601 tattccgttc catacgaata cattcaacaa aatatagata ttcgaatgac agagaatcta
     3661 attgaactat attttaattc catgcgaatc gcaacacata aacgtctgta cgggaaattt
     3721 gggcagtatt cgactgtcag agatcatatg ccagataatc acaaaatgta tgtggaccat
     3781 accccagaat cctcgatgga atgggctgaa accatcggtt cctcaacagt aaaggttgtt
     3841 caacacctct tagaccgtta tcaaaatgaa aaacaagcct taaagtcgat catgtctttg
     3901 aagagctttg gtaaaaagta ttctaaaaat gaaatagaaa cggcctgttc aacagcttta
     3961 aatctggctt ctttgccttc tgttaaaatt atccagacca ttctaaaggg caacaagcag
     4021 aagcgagcaa caagccaatc acatattgaa gacactaaca ttgaaaagca tggattcact
     4081 cgcggtgcgt cctattttgg agggaaataa tgatgttaaa tgagcaaaca gaagtaaaat
     4141 tagctgaatt gaaactttca ggcatgcttg aatcctatcg tgaacagatg acaaataaag
     4201 agtaccaatc catgagtttc gaagaccgct tcaacctcat ggttgatctg gagcattccc
     4261 ggcgaaaaaa taataaacta cagcggttga ttaaatctgc aaactttaga aatgcctctg
     4321 ccagcattga ggatatcgaa taccatccgg accgtaattt agataagaac ctcattttaa
     4381 aactagccag tgggacttat ttggaagaac accataacat cattttgatg ggcgcttcag
     4441 ggaatgggaa aacgtatatc gccaatgcct tcggaataca ggcatgccgg caatactaca
     4501 acgtgaaata catccgactc cctgaactac tagatgaatt aaccatctcg aaaaatcaag
     4561 cagatggtag ttttcgaaaa ataatcaaca gatataaaaa agttcaatta ctcatcatcg
     4621 atgaatggct attaaccaat ctttcgttag aaaattccac ctttctattg gaaataatcg
     4681 aatcccgtct taaaacagca tcaactattt tctgttctca attccatcca gaaggatggt
     4741 acgaaaagct cggcaacccc caaatcgctg atgccattct agataggatc atacatgatt
     4801 cttatcaaat tctggtagac ggtaaagttt ctatgcgtga acgttacggc atccgtaatt
     4861 aaatgagtca ttaaaataaa ttaagagcca ctccatgatt ttcatgagtg gctcttagtt
     4921 gaccttgtgg tcattattgt cgcttttatt atttgaaatg gtaaccgcgt cgcacttact
     4981 ggtacatttc tttgcactta gtggtaaaaa acaatgctct agtggtacat tttcgatgcc
     5041 gtattcaacc agcttgaata aaggcactaa cacctgctgc tgcatcatcg caatggccag
     5101 ataaataatc ccctgtaacc atgcctaata atactcctaa caatccccca gctgtgccaa
     5161 ctaaggtacc tcctactttc aaagcaccag cattatactt caactcgcta cgcaatgcat
     5221 ttaatcctct gtaagaagta gcatatgagt taaccccacc attcacaaat ttagttgtga
     5281 aagcatcttg tgaattaaca acagctgtca attcagctcc atcatatttt tcgactaaag
     5341 aaaaaaattg ctcttcacta atagaattat cttgactaga agtagataat actgattgtg
     5401 tagaaaataa atcagggact gaaacagttg attgattgga agcaatcaat tcagctaact
     5461 cttcttcaaa ctgaatgtaa agatcatttt ttacaatttc ataactaaca gaatcttcct
     5521 gtttttcaga agctttggct attgtaggag taattaaggt cgatgtagta attggcaata
     5581 aggctaaaat agtaaataaa tacttaaatt tatttttcat tttttatcct tctttctatt
     5641 ttttctactt ttaataccac aacttgaaaa ataaaaaatc taagcaagag taacatctct
     5701 tacgtaatga ttttgcaata gtttttcctc ctctcacaat ctattattat gtttttcgag
     5761 ctgtttcaaa agcacttttc tatttacatc tgggaaacta atcgttgctt ttataaaaaa
     5821 agatgatagc agaaaaaatt gaacttttct ttactataag gtatttataa aatcatcttc
     5881 ataggtcaaa gtagtagtat taatctcaac tttgataagc tggtgtatat caattttctc
     5941 tttaatatgt acaatcatta aaaatagatt tttttactat ttatggtttc aattaacaaa
     6001 agaacctcca cactaaatca ctataaattt attatcacct tagtttttta tgtgctccta
     6061 gcatggaaat cccatcaaaa aagtaaggtt tgtattcaaa tgtttttcgc caacgagggt
     6121 atcttctttt ttacactctt tgctgtatca aaatataact gaaattctaa gtctcttcta
     6181 tatcagtgat tatgtgattc gaaatatcca ttccctcaaa agaagttcat atagaaagtg
     6241 aatagagagt ggctcctgta aatatgatat agttaaactg gcaaaataaa atatgctttt
     6301 tcaaaaagta atatttttgt tttaattacg aagaactact tttaaatact gtaagcacct
     6361 tggcttgtta gttaactatt cttaaaatga gagggagaca aatatccatg aataaagcta
     6421 tattttggga tctgttaggt acgctgggag gggatagtag aacactgatt aatcaaaatt
     6481 tcaatttttt taaagacaca ataccggcct taaaaaaagc ggcacaaagt cattatctaa
     6541 atatcatcat tacgaatcaa agtcatattt cacatggacg tattactatg gcagaatata
     6601 ctgatgcctt agagagctta ataaatgagt tagagaaaaa tgacattcaa ataacggatg
     6661 tatacacttg ccctcatgct agaaaagaca attgtgattg caagaagcct caacctacat
     6721 ttgtcaatga agcattaaaa aaatacgatt taaatccaag taagtgtttc gtagtaggcg
     6781 attccggtaa aaacgatatg atgttggcca aaaatgctgg tataaacagt gttttacttt
     6841 taacgggaga gggaattgat agtttaacca aatatagaca tctgtggagt gaaacaaaac
     6901 ctacttttat tgctgaaaac agtttagatg ctataaataa aatacatatg tatgcagatg
     6961 gagactcttc tgattcatga taccagaaga gtctccattt attactaaaa accgtatcaa
     7021 aaacaatcga ttatgagaca aaatgaaatg acctctttta ttaaataaaa gaggtcatat
     7081 tttgtatcat aacttgttgc attaactatg tatctataac tcccttttat gatacaatca
     7141 agccagaatt aaagtacgaa aggaagaaaa gcatgagtgg atacatttat ggttatgccc
     7201 gagtgagtac acgttcacaa gaactaaatc gtcaattaga cttactcaat gaacaaaatt
     7261 gtaatgaaat cctaactgag aaaatgacgg gaaccaaagc ggttcgtccc caactaaatc
     7321 gtttaaaaga taaaattcgt ccgggagata cggtaattgt agaaagtttt tctcgattgg
     7381 gtcgtagtac aaaggatttg attgaccttg ttacctattt tgaagaacac catgttaagc
     7441 tggtgagctt gaaagaaaac ttcgatacca gcacgcctca cggacgctta atgatgactg
     7501 tcttccaagc atttagtcag ttcgaacgtg atttaattgt tgaacgtacc aaagaaggtt
     7561 tgaaaagtgc acgtgcgaga ggtcgtaaag gtggccgtcc acgagtgaac cagaaagatg
     7621 ttgatcgagc agtaaaattg tataaaagtc aggtttatag cgtaaaagaa attacggaaa
     7681 tgactggtat tagcaaggca acactctacc gctacttaaa agacaatagg gaataatcta
     7741 ttttaatctt taggaatcta taatatttat gggagaatta gagtgatttt tttattatta
     7801 ctaaaaaaca atataaaata acgcatttta agccctcttt gtgcataagg atgaagcttt
     7861 taatttgaaa gaggcttaaa atgcgtttta attttttcta tttttcataa atatataggt
     7921 tagtggttta aaattatagg gtattacaac tctcagctgt taaaaattat cttttaaagt
     7981 gtcgtctatc ctctcaagtt gaaacattgg gttcttacct aatctttttt aattgttcat
     8041 aaaaattttc atgcgttcca catagtaaga taggtatcac tgcattatct ttaatttcat
     8101 atgcaacgcg atatgtcgta cctgcatact tgaatccaat agcccaaatt cctgataaat
     8161 cacccgtttt ttgttctccc gaatgaggac gaactgctat ctcatcataa attatcgtta
     8221 aaaattgttc ttttaatgtt ttctctttta atttttttag atactttatt acgtgtttag
     8281 aaggtagaat ctcataagcc aatttctgct gccaactcct cttttgtcat cggtttagaa
     8341 tttgcaacag tatcttcagc aatagattta aatgcagaag ttaacgcatt ttttctattt
     8401 ctaaattctt ttaagatctc ttgtttgcta taaccttctg aaagaatatc agccaaaata
     8461 tcttcactaa aatcaaaaaa ttctttttta ggctctacaa actcataaag gataccattc
     8521 tctacaagct tagcttctac ttcaacacca ttgggcacat cgaattcttt agggaccgtt
     8581 aacataatag aatttccttg ttttcttgtt tttgtaatca tattatttcc tccattattt
     8641 tacatttcgt aattacttag taattacatt gtacgctatt tttagtagaa atacaaaata
     8701 ataaaactcc tttagaacaa ttttcaaagg agttttattt gattaggaaa ttatgtagaa
     8761 taataatatg tttttaactt ggaatcaaaa acgcacttgc aagtaagttt acttcaatac
     8821 atgaaattgc ttataatagt gtattggaat taaggtagat tgacaaaaga gtgatcaaat
     8881 aggtatactt cattcaaata atcgtttgaa tgtgaggtga gatgaatgaa aaaagtttgc
     8941 caagtgactg tcgtacataa agaaaaagta aatggaataa aaaataaatt ggatcaaaaa
     9001 gatttttcaa acttacttgt gttagggaaa tgtttcagcg atttttctag aattaaaatt
     9061 ttctatgctt tagaaaccta taaggaaatg tgcgtctgtg atttagcaga aatacttact
     9121 gctagtgtcg ctacaacgtc acaccattta cgttttttaa aaaaacatgg aatggcaaaa
     9181 tcacgtcaag atggaaaagt agtttattac tctttagcga atgaagacat cctttccgct
     9241 attcgtgttt tttaaagtta tcagaaaatc tatctatgaa atcttagttt gggggtaccg
     9301 tcaggaaaat gcggatttac aacgttaagg aaatttatta aagagaccaa ttcctcagca
     9361 ctaaaattga taattgggct ttctttgtta ctatagtgtt aggtaagctt tttactttat
     9421 caccggtttt cccctcactt cacacctacg ggcgagtttc cccacatccc gttttccatc
     9481 taatccaatt cattcaatat ctttatgtgt atgaaagatc agattgcttg atttagacat
     9541 cttacacgta atttattctt aaatgggtac ggtaaacgag caataatacg agcaaaagta
     9601 tacctacaaa aataataact ctgtctaatt ttttttgatt catatgctta ttgcttaaac
     9661 catataattt cagtccaaga aagcctccaa cagtatttgt aattacatct gttatgtctg
     9721 tcgctccaat agcgaagata aattgaatta tttcaaaagt aagacttaaa accagtacaa
     9781 aagcaaactt aggtaaaaat ccgatttctt taaaattgac attcaaaagc aagccaaatg
     9841 gaataaagat tataacatta tctatcatct ctctgaaatt cccagtagca gtaaatggag
     9901 tcaagttaag acttctttga tgataattaa atactgataa aatattgtat tgtaatttga
     9961 ataacactaa ccagattagt gtcactaaat ataaagctag caatcctcta gataatattt
    10021 ttcccaatat aatttcctct aattcttttg tttaagttgt atcattagta tagcaatgtc
    10081 ctagttaacg tgggttttaa attaaaaagc taacgttacc ccaaaagtgc tagtacaata
    10141 atgctgttca ttaatttcgt tggtttcatt ccgccatcct tacctccttg aattagtatg
    10201 tgttgatccg ggaaacactg tcacaattac accgtctata ttgtttcctg atatctcata
    10261 acgaagatta gtcggcacat gaatggtggt atttttagta acaggataat aaaagatctc
    10321 atatttttcc ccatttacac taacagaaat ggttttttct tcttttaggt aatccatata
    10381 ttcctcgaga acgaaattct tttctttcat aatcgcacta tgtggtaaac caacatagcg
    10441 aatatgccat ggttcatatt gaattcctgt taactctgtt ttgtcctctg gataacgtaa
    10501 aatgaacccg tatttccaag cattttcttc tatccacttt ccttcagggg ctcgttccat
    10561 tttcgtcaag cttgatccta catctagtga taaacctgaa ttatgctcac tataacctgc
    10621 tggtaaggca tactcagccc ccatttcttg gtaaagcaca ctttgctcat caaagtctcg
    10681 atagccacta ttaataataa aatgactaac gccacccttt acagcatcat tgaccatctc
    10741 tgaaaatttt tgtgctattt cttttgacat ataaatatta ctatcaagca acccgtatcc
    10801 atttattaat tcgtcatgtt tagataaatt cacgatatct gacttcacac tttcttggcg
    10861 aacaggatat ttactattga ttaatagcag atttccttga taaacctgtt cttctgtaat
    10921 tgttttctct tgggtatttt cagaagtccc actattttct aaatgttctt tgggattttg
    10981 atcataattt tgaaattcga ctttttcctg agaaaacagt gcttcattaa cgtagtcata
    11041 acctaagtat attaagaata acaataacaa taaaaaaaac aacttcttca ttttcagtct
    11101 cctccttaac tatatagttg gaagtatgaa atatttctag aatcttcccc ttaaacggca
    11161 caatctcgtt tgagattgtg ccgttttgcc ccgtaaatta cagtcaatta tttcgcccta
    11221 ctggatttac tattatcacc aatgtagatg aattttcata agtga
//
LOCUS       DACGLE010000084        10813 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0534
            SAMEA3304449-rid9560453.denovo.089, whole genome shotgun sequence.
ACCESSION   DACGLE010000084 DACGLE010000000
VERSION     DACGLE010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304449
            Sequence Read Archive: ERR985064
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10813)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10813)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702225.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702225.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 20:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,045
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 27 of 67
            Pseudo Genes (incomplete)         :: 25 of 67
            Pseudo Genes (internal stop)      :: 19 of 67
            Pseudo Genes (multiple problems)  :: 4 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10813
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304449-rid9560453.denovo.089"
                     /strain="VREN0534"
                     /db_xref="taxon:1352"
     gene            <1..571
                     /locus_tag="IXT20_002369"
     CDS             <1..571
                     /locus_tag="IXT20_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HAQ4860794.1"
                     /translation="ASRIAIMSEGRVIQFDTPENILRHPANDFVEELIGEDRLLQAKP
                     NATRVGEVMLNTAITITPEKSLQEAIKLMREKRVDTLLVTDNSNVLKGFIDVETLNKK
                     RGKVSSVGDILNKHVFYVKESAYLRDTLQRILKRGLKYVPVVDEQNKVVGILTRASLV
                     DIVYDVIWGEDPATTDVAESATPEESKEV"
     gene            575..1216
                     /locus_tag="IXT20_002370"
     CDS             575..1216
                     /locus_tag="IXT20_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ4860795.1"
                     /translation="MQQFFHQHGSEILSKGFEHLYISGLALIIGIIIAVPLGVLLTRF
                     PKAATIVIGLTSALQTIPSLALLALMIPLFGVGKLPAIIALFIYSLLPILRNTYIGMK
                     NVDWNYRDVAKGMGMTEFQSIFSVELPIAMPTIMAGIRLAAVYVIAWATLASYIGAGG
                     LGDLIFSGLNNYQPDLILAGTIPVTILALLADWLLGLLEHRLTPIALREENEE"
     gene            1213..2130
                     /locus_tag="IXT20_002371"
     CDS             1213..2130
                     /locus_tag="IXT20_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmoprotectant ABC transporter substrate-binding
                     protein"
                     /protein_id="HAQ4860796.1"
                     /translation="MKRILKMTMVLFSLLILASCSFPGLASNTDEDTISITGGITSEA
                     QILASLVAGMVEHYTDKNTAIINNLATTTINHQAMMNGDASISAARYTGTDLTTTLNL
                     PPEKDPKKAFATVKDEFEKRYGQTWFPSYGFENTYVFLVRKDTAQKYHLSKVSDLKNV
                     ADELVAGVDTSWINRKGDGYDGFQETYGFSFNSILPMQIGLVYDAVEAGKMDIVLGYS
                     TDGRIASYDLVMLEDDRQFFPPYDAAPVVDNKILADTPHLKEALNKLGHTISTEKMQE
                     LNYEADNNLVEPSVVAQRFLQENHYFEGK"
     gene            2127..2798
                     /locus_tag="IXT20_002372"
     CDS             2127..2798
                     /locus_tag="IXT20_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ4860797.1"
                     /translation="MKQLDKMNVFQQFFYYFQENGSYIFAQFIRHFLISIYGVLFAAV
                     VGIPVGIMISRRRKLANWVIRLANIIQTIPSLAMISILIIGLGLGVNVVIVTVFLYSL
                     LPIIKNTYTGMIQVDKNILDVGKGMGMTARQRLFMVELPLSVSVIMAGIRNALVVAIG
                     ITAIGAFVGAGGLGDIIIRGTNATEGTSIILAGALPTALMAIITDWLLGILERRLDPA
                     SRTSR"
     gene            complement(3339..3827)
                     /locus_tag="IXT20_002373"
     CDS             complement(3339..3827)
                     /locus_tag="IXT20_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-glycoprotein
                     endo-beta-N-acetylglucosamidase"
                     /protein_id="HAQ4860798.1"
                     /translation="MTRKISKIGASLLVLAVATTIDYRQSEAKTIGNVVYRETAKSML
                     KKAMDNQPESSYWFPEDSLAWSFGKDTDTKYNTSVVPLAERVSKATLPNMNVTQTEEV
                     KVVALSAMNSSINGNASRGIDIFYANVFSYWQYIDQLVYGGALLVKDHRIAKSSCDGC
                     SP"
     gene            complement(4028..4294)
                     /locus_tag="IXT20_002374"
     CDS             complement(4028..4294)
                     /locus_tag="IXT20_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HAQ4860799.1"
                     /translation="MSNYTVAIKNSAKVDLRKIKQTNLKKQFEEVIQTLKEDPYMPTQ
                     SFEKLRPTHEGRYSRRLNRQHRVVYKVDEENKVVEIYSAWTHYE"
     gene            complement(4294..4557)
                     /locus_tag="IXT20_002375"
     CDS             complement(4294..4557)
                     /locus_tag="IXT20_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HAQ4860800.1"
                     /translation="MANTTMNPSTARKNFYQLLKEVNENHTEIEIISDRSGNNAVLIG
                     LEDWRAIQETLFLEQTGTLDKVRDREKDDSSFTNIDDIDWEAL"
     gene            complement(4550..4822)
                     /locus_tag="IXT20_002376"
     CDS             complement(4550..4822)
                     /locus_tag="IXT20_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4860801.1"
                     /translation="MYWCCCIDSQALDSTFAKDDTLLPITLRLATASRKGVRSERGQR
                     SSLKRGLFLYVKKRKSLLNLSALLYFFDVLNQVRYNYIRKRRRDNG"
     gene            5033..5218
                     /locus_tag="IXT20_002377"
     CDS             5033..5218
                     /locus_tag="IXT20_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAQ4860802.1"
                     /translation="MALSYGNKKWSLDEAEIPTYEDTWHLRVEVKEEAENIGGCTLFG
                     KGFGNYDQDIKIKMFYS"
     gene            complement(5360..7183)
                     /locus_tag="IXT20_002378"
     CDS             complement(5360..7183)
                     /locus_tag="IXT20_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HAQ4860803.1"
                     /translation="MDYLKRLLVGKPLKSAENDEHKLTRFAALALLSSDALSSIAYGT
                     EQIVVVLVALSAAAIWYSLPIAAFVIILLISLTLSYRQIIHAYPHGGGAYVVSSENLG
                     KNAGLISGGSLLIDYMLTVAVSVSAGAEAITSAVPALYGHQVAISVTIVLLLMMLNLR
                     GLRESASFLLFPVYTFILVISLLIVVGLYNIVTGAVPLQATALPGAAVPGVSIALILR
                     AFSSGSSSLTGVEAISNAVPFFKKPRAKNAAATLTMMALILGFFFVGITFINYWYGIV
                     PEKEVTVLSQIGKAVFGHGILYYVLQFATALILAVAANTGFSAFPVLAYNLAKDKFMP
                     HMYQDRGDRLGYSNGIITLALGSIVLLFIFHGSTERLIPLYSIGVFIPFALSQTGMVV
                     KWKKEGKRWLSKSIANITGAFISYAIIAILFVYRLGDIWPFFIIMPIVMFIFYKIHDH
                     YKKVAEQLRLENDAKLHDYDGNTVLVLVGNVTRVNIGALNYARSIGDYVVAMHVSLDE
                     DVEKEKEIEAEFKKHFPDVRFSIVHSSYRSIENPIIRYVDIVSKNAAKQNYTTTVLIP
                     QFVPNRRWQNILHNQTSLRLRLRLSWRENIVVSTYSYHLKK"
     gene            complement(7781..10486)
                     /locus_tag="IXT20_002379"
     CDS             complement(7781..10486)
                     /locus_tag="IXT20_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip domain-containing protein"
                     /protein_id="HAQ4860804.1"
                     /translation="MQSIKNTLRLYLLDWKRIFKNPIATLLIIAIMIIPSLYAWFNIK
                     ALWDPYGNTGELPIAVYSADKPAEFQGKEVAIGEQVIESLHKNKQLGWQFVDSKEQLE
                     DGVRSGKYYAGIYLPKDFSEDLLSFTSGDIKKPKIEYTVNEKINAIAPKITDKGASSI
                     QSQITNEFIKTASSTLLKVFNEIGYDIDTNLVSINKVKDMILSTDENLDTIDGYTKQV
                     LELQSQLPEIKEKLNKANEFVDYIPKVDEMGEKVVALNDKMPELKDQAKIILDLQEKI
                     PEIQNAGKQLAEIDGDFASIQETMNQGIDEAKQGLTIIQQVQDILPDVKNLENQASDL
                     ANQTKSGASQLKDAIPSITNSVDVTLKSIGQVATTTGSLIDTVRTAIEDGKLTDDEKK
                     NISNVLNGFIDGISQQQNAIDKLVEFLTKLQTSAGNTKLQPIIDQLNQLKPLLSNLQN
                     RLSALNTAVQNEDTKAIDELLDQIDAVVNNINGIIAGINTGQISSTVTSLLDQVIDTI
                     GTAQGALSKADQIDFDSLLSSTKATVTNAVAILEKYQKEMPAIGQEIHDANTMLNGNM
                     DTIVNGINEGADLYNNELPIIQQKLGLAADFIQNDWPALKNEITGTLTTVNEKMPEVE
                     TAVNAAADLINNDWPNIKAGIQKAADAIRKGEQEVDLGEVIKLLKLDATKESDFFTQP
                     VELQTNALYPIANNGSASTPFYTALCLWVGAVLLSSVATTEYYLGKKDRNNFTKREQF
                     VARMLTFLTMAIAQSLIVTLGNIFLLGVDVQNPVYSVSFAVLIALAFMMIVYVLAALF
                     DNVGKGIAIIILVLSISGGGGNYPIQVSGKFFQMINPFLPFTHAVNLLRESAGGIYWP
                     NATKAIWIMIGLFVVFGIVGTAIYPFIEEKMTKFKEAAHESHIFH"
BASE COUNT         3105 a         2240 c         2042 g         3426 t
ORIGIN      
        1 agcaagccgt atcgccatca tgagtgaagg ccgtgtgatc caatttgata caccagaaaa
       61 tattttacgt catcctgcaa acgatttcgt agaagagttg attggagaag atcgcctact
      121 acaagccaag ccaaatgcga caagagtcgg cgaagttatg ctaaatacag caatcacgat
      181 tacaccggaa aaatcattac aagaagcaat caaactgatg agggaaaaac gtgtagatac
      241 gttgttagtt accgataatt cgaatgtatt aaaaggattt atcgatgtag aaacgttgaa
      301 taaaaaacga ggaaaagtat ccagtgtcgg cgacattttg aataaacatg ttttttacgt
      361 aaaagaatcc gcctatcttc gtgatacctt gcaacgcatt ttaaaacgcg ggctgaaata
      421 cgtgccggtc gttgatgaac agaacaaagt cgtaggtatc ctgactcgtg catcattagt
      481 ggatatcgtc tacgatgtga tttggggaga agatcccgca actactgatg ttgcagagtc
      541 agctactcct gaagaatcta aggaggtctg acctatgcag cagttttttc atcagcacgg
      601 ttcagaaatt ctctcaaaag gttttgaaca tctttatatt tcaggacttg ctttgatcat
      661 tggtatcatc attgctgtac cgcttggtgt cttactgaca cgttttccaa aagctgccac
      721 gattgtgatt ggcttaacca gtgccttgca gacaatccct tctcttgctt tattggcatt
      781 gatgatcccg ctatttggcg taggtaaatt acctgcgatt atcgcactct ttatttattc
      841 attattgccg attttacgta atacgtacat tgggatgaaa aatgtcgatt ggaattatcg
      901 ggatgtggca aaaggcatgg ggatgactga gttccaatcc attttttccg ttgaattgcc
      961 cattgctatg ccaacgatca tggctggtat tcgtttagct gccgtttacg tgattgcatg
     1021 ggctacactt gcctcttata tcggcgctgg tggtttaggc gatttgattt ttagtggact
     1081 gaataattac caaccagatc tcattttggc aggtacgatc cctgtaacaa ttttagcact
     1141 attggctgat tggcttctag gtctcttgga acaccggctg acaccgatcg cacttagaga
     1201 ggagaatgaa gaatgaaacg tatactaaaa atgaccatgg ttcttttctc tcttcttata
     1261 cttgcgagtt gttccttccc tggtcttgcc agcaatacag atgaagacac catttcgatc
     1321 acaggtggaa tcacttcaga agcacagatc ttagcaagtc tagttgccgg aatggttgaa
     1381 cattatacag ataaaaatac cgcaatcatc aacaatctgg ctactacaac tatcaaccat
     1441 caagcgatga tgaacggaga tgcttcaatt tcagcggctc gctacacagg aacagattta
     1501 acgactacat tgaaccttcc tcctgaaaaa gatccgaaaa aagcgtttgc taccgtgaaa
     1561 gatgaatttg agaaaagata cggtcaaacg tggttcccgt cctatggttt tgaaaatacg
     1621 tatgtctttc ttgttcgaaa agacaccgca caaaaatacc atctatctaa agtcagcgat
     1681 ctgaaaaatg tagcggatga attagtagct ggtgtagata cttcttggat caatcgtaaa
     1741 ggagacggtt atgacggctt ccaagaaact tatggcttct ccttcaattc gatattacct
     1801 atgcagattg gacttgttta tgacgcagtc gaagcaggaa aaatggatat tgttcttggc
     1861 tactctacgg atggacggat cgccagttat gatctagtca tgttggaaga tgatcgtcaa
     1921 ttcttcccac catatgatgc tgctccagtt gtagacaaca agatattagc tgatacgcct
     1981 catttaaaag aagccttaaa taagttaggt cataccatca gtacggaaaa aatgcaggaa
     2041 ttgaactacg aagcagataa taatctagtg gaaccatcag tagtagcaca acgtttctta
     2101 caagaaaacc actattttga agggaagtga agcaattgga taaaatgaat gtctttcaac
     2161 aatttttcta ttatttccag gaaaatggct cctatatctt tgcacaattt atccgtcact
     2221 tcctgatttc tatctatggt gtcctctttg cagcagttgt cgggattccg gttgggatca
     2281 tgatttctag gagacgtaaa ttagcaaact gggtgattcg tttggcgaat atcatccaga
     2341 cgatcccttc tctagccatg atctccattt taatcattgg attaggcctg ggagtcaatg
     2401 tcgtgattgt tactgtattt ttgtattccc tcttaccgat catcaagaat acgtataccg
     2461 ggatgatcca agtagacaaa aacatccttg atgtcggcaa aggcatgggg atgactgcta
     2521 gacaacgact attcatggta gagttaccac tctctgtttc tgttatcatg gcaggcataa
     2581 gaaacgcgtt agttgttgcc atcgggataa ccgcgatcgg tgcattcgtc ggtgctggtg
     2641 gtcttggaga tatcattatc cgaggaacca atgcaacaga gggcacatcg attattttag
     2701 caggtgcatt gccaactgca ttaatggcga tcatcacgga ctggctttta ggtattttgg
     2761 aaagacggct agatccagcc agccgtacat cacgctgatg ttattgtaaa aaacaaaagc
     2821 ttctgcgacc atttgttggt cgcaaaggct tttttagttc tgtcattttt aaggcaataa
     2881 tccactctga ttcttccctc cttatgggca aagagttgct tttcttagct atttatccat
     2941 tccgtatact ccaacttcat actgccaacg ggttcatatc tgcatccacc gactaagttt
     3001 ttttcttatc cgctaaggca ttttgccaat ccattttccc atcactcgtc atggaattca
     3061 taccacatga tctctaaata ctcgtctgct tgttctttga attgggcatc aattcttaca
     3121 tcctttggcg tggctttcat ttaagccttt tccatcagtc ctcgattgtt cgatgccttc
     3181 agctgcatca tcaaaactgg tgactgccgt atcggtaact tcgatcatct tttatacaat
     3241 cgggaacttg ccatcctcat ccttggctaa aaacatatct agccattcca gcttctcgcc
     3301 atgggaggtt tgcggttcct aaaaccggta cacgattctt atggactgca tccgtcgcaa
     3361 ctagacttgg cgatacgatg atccttcacc agaagagcac ccccgtaaac caattgatcg
     3421 atatattgcc aataagaaaa gacattggcg taaaagatat cgatccctcg tgatgcattg
     3481 ccgttgatac tgctattcat ggccgaaagc gccacgactt taacttcttc cgtttgagtg
     3541 acattcatgt tcggcaatgt cgctttggat acccgctctg ctaaaggaac gacactggta
     3601 ttgtatttcg tatctgtatc ttttccaaaa gaccatgcca acgaatcctc tgggaaccaa
     3661 taagacgact ccggttgatt gtccattgct tttttcaaca tacttttggc tgtttctcga
     3721 taaacgacat ttccaattgt cttcgcctca ctttggcgat aatcgatggt cgttgccact
     3781 gccaacacta acaacgatgc tccaatttta ctgattttcc ttgtcacact tcttcctcct
     3841 tgttttacat aaacccgtca acattccatt cctaaactca aacaatgacc cttcaaaggt
     3901 ggagatatag gaacaaatta aagaaatcat gcaataaatc caagaaggtt ggttttagaa
     3961 caaaaaaaga gtctcctttt tggggagact ctttgttaac tatctaaaaa ggttggctct
     4021 tctcacttca ctcataatgt gtccaggcag aataaatttc tacaacttta ttttcctcat
     4081 caactttgta aaccactcga tgctgtctat tcagcctgcg tgagtatctg ccttcatgtg
     4141 taggtctcag cttttcaaaa gactgcgtgg gcatatatgg atcttccttc aatgtctgaa
     4201 tcacttcttc aaattgtttc tttaaattcg tttgcttgat ttttcttaaa tccactttcg
     4261 cagagttttt gattgcgact gtatagttac tcatcaaaga gcttcccaat caatatcatc
     4321 gatattcgta aagctactgt cgtctttttc gcggtctcgt actttatcta aagtacctgt
     4381 ttgctcaaga aaaagggttt cttgaatcgc tctccagtct tccagcccaa tcaatacagc
     4441 attgtttcca ctacgatcac taataatctc gatttcagta tgattttcat ttacctcttt
     4501 caataactga taaaaatttt tacgagcagt gctaggattc atcgttgtat tagccattgt
     4561 ctctcctcct ttttctgata taattatagc gtacttgatt aagtacgtca aaaaaataca
     4621 ataaagcact taaattcagc aaacttttcc tcttttttac atagagaaaa agaccccttt
     4681 ttagggagga tctttgtcca cgctcacttc ttactccttt acgactagcg gtagccaaac
     4741 gtaaagtaat tggtaacaac gtatcatctt tagcaaaggt cgaatccaaa gcctgtgagt
     4801 ctatgcaaca acaccaatac acgaaggttc ctcgttgcag tgtgacttga ttgatatctt
     4861 gaataacctg aggatacgca cgaaagattt gtaacggtta ttcaaaactg attttggccg
     4921 tcaagtcttc tactgcctca aaaatgattg cctctttcgt ctcctgtcat tcatgttcac
     4981 tatcgatttg atacttgctt agaccgttgg gtttgtgaat caccaattaa aaatggcact
     5041 gagttatggt aacaaaaaat ggtcacttga cgaggctgag attcctactt atgaagacac
     5101 atggcactta agagtcgagg taaaggaaga agctgaaaat ataggaggct gtacattatt
     5161 tggcaaagga ttcggaaatt atgatcaaga catcaaaatc aaaatgtttt acagctaaat
     5221 aggaagaata ataacatttc atacgctgtt cccatcttta atggcttata gagggcacct
     5281 ttagctgaga ataagacaaa acagaccctc tagcttcaat taagagctag agggtctgtt
     5341 tttgatttta aaacttggtt tatttcttca aatggtagct ataggtgctg acaacgatat
     5401 tttcacgcca agacaatcgc aatcttaacc gcaaacttgt ttggttatgg agaatattct
     5461 gccagcgtct gtttggtaca aattgaggaa tcaacacagt agttgtatag ttttgtttcg
     5521 ctgcattttt acttacgata tctacataac ggataattgg attttcaatc gagcgataag
     5581 aagagtggac gattgaaaaa cggacatctg ggaaatgctt tttaaattct gcttcgattt
     5641 ctttttcttt ttcgacatct tcgtctagcg aaacatgcat cgcaacgaca taatcaccga
     5701 tcgaacgtgc gtaattcaac gcaccgatat ttacccgtgt cacattcccg actaatacaa
     5761 gaacggtatt cccgtcataa tcatgtagtt ttgcatcatt ttctaaacga agctgttctg
     5821 ctactttttt ataatgatca tggatcttat aaaaaatgaa cataacgatt ggcataatga
     5881 tgaagaaagg ccaaatgtcc cctaaccgat agacaaacaa aatcgcaatg attgcatatg
     5941 agatgaacgc acccgtaata ttggcaatcg atttgcttaa ccatcttttt ccttcttttt
     6001 tccatttgac gaccatccct gtttgagata aagcaaatgg aatgaacacg ccgatagagt
     6061 ataacggaat caagcgttct gttgagccat ggaaaataaa cagcaatacg atcgaaccta
     6121 gcgctagtgt gataatccca ttggaatagc ctagtcgatc cccacgatct tgatacatgt
     6181 gaggcatgaa tttatctttt gccagattgt atgcaagaac tggaaaagca gaaaaaccgg
     6241 tgttagcagc aactgctaaa atcagcgctg tagcaaattg aaggacgtaa tacaaaatac
     6301 catgtccaaa gacagcttta ccaatttgag acaaaacagt gacttctttt tcaggaacga
     6361 tcccatacca atagttgata aatgtgatcc ccacgaagaa gaaacctaag atcaatgcca
     6421 tcatcgtcaa tgtagcagcg gcattttttg ctcttggctt cttaaagaat ggtacagcat
     6481 tactaatcgc ctccacccca gttaaagagg aagaaccaga agaaaatgct cgtaaaatca
     6541 aagcgataga aacacccggc acagccgctc ctggaagtgc agtagcttgc aatggcacag
     6601 cacctgtcac gatattatat aaaccaacaa cgatcagtaa cgagatcaca aggataaatg
     6661 tgtacaccgg aaataatagg aacgatgcag attccctcag cccacgaaga ttcagcatca
     6721 tcagtagcaa aacgattgtc acagaaatag cgacttgatg accgtagagt gctggcaccg
     6781 ctgatgtgat cgcttcagca cctgctgaga cagatactgc tactgtcaac atgtagtcga
     6841 tcaataaaga gccaccggaa atcaagccag cattctttcc aagattctca ctgctgacga
     6901 catatgctcc cccgccatga ggataagcat ggatgatttg acgatatgat agcgttaatg
     6961 aaatcaataa aataataaca aaagcggcaa tgggtagcga ataccagatg gctgcagcag
     7021 acaaagcgac taaaacgaca acgatttgtt ccgtaccata tgcaatagat gataacgcat
     7081 cagacgacaa taatgccaaa gcagcaaatc gggttaattt gtgctcatca ttttcagcag
     7141 attttaaagg cttacctact aacaaccttt ttaagtaatc caatttctaa actcctttac
     7201 acacaatttt acttcaagtt ttatagtcga aaatttatag taaacaagat gttatagtac
     7261 gctcactgac cttaaaagtc aataaacttt aagaaaataa ttggaaaaaa acagaaaaat
     7321 atcaagcgct ttttcaaacg ctttaataca gcaaaaatct aacatttttt tatgggcttt
     7381 ttctttgaat tctataataa aaggatagaa cttgacttag aaaatcaagt tctatccttt
     7441 ttgatatctt aacttttgga agagagatgt aaggctgctt tctacccatc attcttacta
     7501 ctcgaagcat agcgcagttg tcacaacctc ttgataaacg gtgtttaaaa gcttgcatct
     7561 gcggtaataa gctcaaccaa attaaaaaca taagaagctg tttttatttg gtcgttctta
     7621 ttacttgatg cagagcagct tttttacaac ctcttggtac acggtgttca atcagctgac
     7681 cctcggtatt agctcaaaaa aacggcaaaa tatgaggaac tatttttcca tttttttctc
     7741 taatactcag gtcaaaacac tgatttcaca acctcttttt ttaatgaaaa atatggctct
     7801 catgcgccgc ttctttgaat ttcgtcattt tttcttcgat gaacggatag attgccgttc
     7861 ctacgattcc aaagacaaca aacaaaccga tcatgatcca gattgctttc gtagcatttg
     7921 gccaataaat tcctcctgca gattcccgta acaagtttac cgcatgagta aatggcaaga
     7981 acggattgat catttggaag aatttccctg agacttggat cggatagttc ccgcctccgc
     8041 cagagatcga cagtactaag atgataatag cgatcccttt accgacatta tcgaataatg
     8101 cagccaagac atagacgatc atcatgaacg ctaaggcgat caagacagca aatgacacgc
     8161 tgtagaccgg attttggaca tctactccta gtaagaaaat attccctaat gtaacgatca
     8221 agctttgcgc aatcgccatt gtcaaaaatg tcagcatacg agcaacgaat tgttctcgtt
     8281 tcgtaaaatt gtttcggtct ttctttccta aatagtattc tgtcgtagca acacttgaaa
     8341 gaagaacagc gccgacccac agacatagtg cagtataaaa cggggtgcta gctgaaccgt
     8401 tatttgcgat tggataaagc gcattggttt gtagctcaac aggttgtgta aagaagtcac
     8461 tttcttttgt tgcgtctagt ttcaataatt tgatgacttc gcctagatcg acttcttgtt
     8521 cgccttttcg gatcgcgtct gctgcttttt ggataccagc tttgatattt ggccagtcat
     8581 tgttgatcaa gtccgcagca gcgttcacag ctgtttcgac ttctggcatt ttttcattta
     8641 ctgttgtcaa tgtaccggtg atttcatttt ttagtgctgg ccaatcattt tggataaaat
     8701 cagcggccaa tcctaatttt tgctgaatga tcggtaattc attgttgtat aggtctgccc
     8761 cttcattgat gccattgacg atggtatcca tatttccatt cagcattgta ttggcatcat
     8821 ggatttcttg tccaattgca ggcatttctt tttgatattt ttctaaaatt gccactgcat
     8881 tcgttacagt tgctttcgtt gagctgagaa gcgaatcaaa gtcgatctgg tccgctttgc
     8941 tcagtgcgcc ttgtgcagtc ccaatcgtat cgatgacttg gtctagtaga gaagtgacag
     9001 tggaactgat ttgacctgta ttgataccag caatgatgcc attgatatta ttaacaacgg
     9061 catcaatttg atctagcaat tcatcaatag ccttcgtgtc ttcattttga acagctgtgt
     9121 tcaaagcaga taggcggttt tgcagattgc ttagcaaggg ttttaattga tttagctgat
     9181 caataattgg ctgtaatttc gtatttccag cacttgtttg caatttagtc aggaactcga
     9241 ccagtttatc gattgcattt tgttgttgag atattccgtc aataaagccg ttcaatacat
     9301 tgctgatgtt tttcttttca tcatcagtca gcttaccatc ttcaatagct gttcttacag
     9361 tatctattaa cgaacctgtc gttgttgcca cttgtccaat tgacttcaat gtaacatcta
     9421 cactgtttgt gatgcttggt atcgcatctt tcagctgaga ggcacctgat tttgtttggt
     9481 ttgctagatc acttgcctga ttttctaaat ttttcacatc tggcaagata tcttgtactt
     9541 gttggatgat cgtcaagcct tgttttgctt catcgattcc ttggttcatt gtctcttgga
     9601 tcgaagcaaa atcgccgtcg atctctgcta gttgcttacc ggcattttgg atctctggga
     9661 ttttttcttg caaatcaagg atgatttttg cttgatcttt caactcaggc attttgtcat
     9721 tcaatgcaac gactttttca cccatctcat ctactttagg gatgtaatcc acgaattcat
     9781 ttgctttatt cagtttttcc ttgatttcag gtagttgact ttgtaattcg aggacttgtt
     9841 ttgtgtagcc gtcgatcgtg tccaaatttt catctgttga taaaatcata tctttaactt
     9901 tattgatact tactaggttc gtatcgatgt cgtaacctat ctcattgaat actttcaata
     9961 aagtcgaact ggctgttttg ataaattcat ttgtgatttg cgattggatc gaacttgctc
    10021 ctttatcggt gattttcggt gcaatcgcat tgattttttc atttaccgta tattcgattt
    10081 ttggtttctt gatatcacca ctagtaaaac tcagcagatc ttctgagaaa tcctttggca
    10141 agtaaatacc tgcgtaatac tttccggaac ggactccgtc ttctaattgt tcttttgaat
    10201 caacgaattg ccatcctaat tgtttatttt tatgaagcga ttcgatgact tgctccccaa
    10261 tggccacttc tttaccttga aactctgctg gcttgtctgc actgtaaaca gcaattggca
    10321 gctctccagt atttccatat ggatcccata atgctttgat gttaaaccac gcatatagcg
    10381 aagggatgat catgatcgcg atgatcagaa gtgttgcaat cgggtttttg aaaatccgtt
    10441 tccagtcaag caaatataat cgaagcgtat ttttgattga ctgcatagtt ctcccccttc
    10501 ttcttttttc agctgaatta ctttaccatg tatttcatac ttttttcaat cggctttttt
    10561 caaaaaagtc gaatttttgg acatatcacg gcaattgtct cattgtaatc tcttacatat
    10621 tgagaaataa ttaaggatta cagcctttac tttatcatga aataaaaggg aaagataatt
    10681 ttatgccaaa gtttttatct attatacgta tacgcaaaaa agctaaaaca ttttttgtct
    10741 ttgcccctac aaaaatgttt cagctttttt cgttttttat tcctgtattt cttatttttc
    10801 gaatgacagg atg
//
LOCUS       DACGLE010000085        10740 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0534
            SAMEA3304449-rid9560453.denovo.090, whole genome shotgun sequence.
ACCESSION   DACGLE010000085 DACGLE010000000
VERSION     DACGLE010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304449
            Sequence Read Archive: ERR985064
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10740)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10740)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702225.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702225.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 20:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,045
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 27 of 67
            Pseudo Genes (incomplete)         :: 25 of 67
            Pseudo Genes (internal stop)      :: 19 of 67
            Pseudo Genes (multiple problems)  :: 4 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10740
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304449-rid9560453.denovo.090"
                     /strain="VREN0534"
                     /db_xref="taxon:1352"
     gene            complement(57..2654)
                     /gene="adhE"
                     /locus_tag="IXT20_002380"
                     /gene_synonym="adhC"
     CDS             complement(57..2654)
                     /gene="adhE"
                     /locus_tag="IXT20_002380"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HAQ4860805.1"
                     /translation="MAKVIEKEKTKTIDVQAMIDDLAEKANAALKEMESFDQEKVDHI
                     VHEMAMAALDQHMPLAKMAVEETGRGVYEDKAIKNMYASEYIWHNIKHDKTVGVINED
                     VQKGLIEIAEPVGVVCGVTPTTNPTSTTIFKSMIALKTRNPIVFAFHPSAQKSSAEAA
                     RVVRDAAIAAGAPENCIQWIEHPSIEATSMLMNHPGIAIVLATGGAGMVKSAYSTGKP
                     ALGVGPGNVPAYIEKTAKIKRAVNDLIVSKTFDNGMICASEQAVIVDKEIYAAVKAEF
                     QAHQVYIVKPDELQKLEDAVMNEGKYAVNPSIVGHSAMEIAKLAGISVPKGTKMLIAE
                     LEGVGPDYPLSREKLSPVLAMIKANNTDHAFDLCEGMLNLGGLGHTAVIHSENEELHV
                     KFGLRMKACRILVNTPSAEGGIGDIYNEMIPSLTLGCGSYGKNSVSRNVSAVNLINVK
                     TVAKRRNNMQWFKLPPKIFFEKNSLLYLEKMENVERVMIVCDPGMVQFGYCDTVREVL
                     ARRKNDVKIEVFSDVEPNPSTNTVYAGTKLMADFKPDTVIALGGGSAMDAAKGMWMFY
                     EHPDTSFFGAKQKFLDIRKRTYKIDKPEKTQFVCIPTTSGTGSEVTPFAVITDSETHV
                     KYPLADYALTPDVAIVDPQFVMSVPASVTADTGMDVLTHAIESYVSVMASDYTRGLSL
                     QAIKLVFDYLEKSVKTPDMESREKMHNASTMAGMAFANAFLGICHSVAHKIGGEYGIP
                     HGRTNAILLPHIIRYNAKDPQKHAMFPKYDYFRADTDYADIAKFLGLKGETTEELVEA
                     LATAVYELGKSVGINMSLKAQGVTQETLDTTVDRMAELAYEDQCTTANPKEPLISELK
                     QIIIDAYNG"
     gene            complement(2959..3339)
                     /gene="yajC"
                     /locus_tag="IXT20_002381"
     CDS             complement(2959..3339)
                     /gene="yajC"
                     /locus_tag="IXT20_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HAQ4860806.1"
                     /translation="MGTLPMLVMFIALLAMWFFMSRSQKKQQQERQNLLDSMKPGDSV
                     VTIGGLHGVVSEIDNDKRTVVLDCEGIFLEYDRAAIKTVKPGTVVTNDATTTVETTEE
                     PAETVEVKTETTETLEETEEKEEK"
     gene            complement(3436..4581)
                     /gene="tgt"
                     /locus_tag="IXT20_002382"
     CDS             complement(3436..4581)
                     /gene="tgt"
                     /locus_tag="IXT20_002382"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010769586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HAQ4860807.1"
                     /translation="MTQPAIRYRLIKKEKHTGARLGELITPHGTFPTPMFMPVGTLAT
                     VKTMSPEELKEMGAGVILSNTYHLWLRPGEDLVEEAGGLHKFMNWDQPILTDSGGFQV
                     FSLSDMRNIEEEGVHFKNHLNGSRMFLSPEKAINIQNKLGSDIMMSFDECPPFDESYD
                     YVKKSVERTSRWAERGLKAHANPDRQGLFGIIQGAGFEDLRRQSAKDLVGMDFPGYSI
                     GGLSVGEPKSEMNRVLEFTTPLIPENKPRYLMGVGAADSLIDGVIRGVDMFDCVLPTR
                     IARNGTCMTSQGRLVVKNAKYAHDFRPIDEKCDCYTCKNYTRAYIRHLIKCDETFGIR
                     LTSYHNLYFLLNLMKQVRQAIMDDNLLEFREAFFEEYGFNKENAKSF"
     gene            4799..5497
                     /locus_tag="IXT20_002383"
     CDS             4799..5497
                     /locus_tag="IXT20_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HAQ4860808.1"
                     /translation="MQSSAELKRQAKESLKGRWGQAVLLNLIPTLIGIATILLIMIPI
                     ILFATTIYDGSASVSEATTSGGGSSGGGGGIISSIISALFMSGISWTYLDILRGQKDQ
                     IEPFKDAFRGFKGLFIGGVISLAVLISIFTTLWGLLLVIPGIIKSYSYSQSYFIYYDI
                     VQETGQTPKVLDTITASRKLMDGYKGQLFWLDLSFIGWHILALATAGIGYLWLSPYIS
                     ATKAAFYEQLPKDI"
     gene            complement(5665..8304)
                     /locus_tag="IXT20_002384"
     CDS             complement(5665..8304)
                     /locus_tag="IXT20_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HAQ4860809.1"
                     /translation="MEEYKQTIEEVKKVTEADPDTGLSSQEVDERRKKQGLNKFDEAP
                     KESMIKKFFRSLSDFTTIILLVAAVISFYTAFATEHGDLFEGLLIIAIVVINSVLAIV
                     QEGNAEKALESLQDMNKQTATVIRDGKVTTVESEQLVVGDILVLESGDAISADARLIE
                     ASQLRVEESALTGESEAVEKDAAFIAEEDESLGDQLNMVFKGCTVAAGRGKAIVTAIG
                     MATEMGKIADLLNENTMQKTPLQVRLNQLGKRISMIALAAAALVFVIGELQGEPLLEM
                     FMTSISLAVAAVPETLTVIVTLTLAYGVQKMARKHAIIRQLPAVETLGTANVICSDKT
                     GTLTQNEMRVRRVWSKEDEVTNIEDSMTNSAMEILKMAALCTDVTVEQEDDDLVIKGN
                     PTEVAIVRAVEENYHTKAELEEKYPRVNELPFDSERKMMTTVHQMGEKYISITKGAFD
                     VLAPRFSSGDVEQAAIVNDRFGKRALRVIAVGYATYDEEPQDISSEALEKDLRLIGLI
                     GMIDPPRPESKGAIKRAKKAGIKTVMITGDHVVTASAIAKELGILNDPSEALSGSELH
                     QLSDEELDARVKALSVYARVTPEDKIRIVKSWQRTGAVVAMTGDGVNDAPALKASNVG
                     CAMGITGTDVAQSAADMILTDDNFATIVDAVSQGRSVYQNIRKAINFLLSCNISEIFI
                     VLIAMLLGWGAPFTAVQLLFVNVVADGLPGFALGREPAEHGIMDQPPIPKNEGIFARG
                     LLQKIAINAGVFTIVTLFGYYLGSYVDTISPWVDASQHVGQTVAFLILAYSSILHVFN
                     VRSSQSIFKVNLATNKALVEMALLALAITTAVALLPFTQELFGLVHISLNHWFLVGIL
                     SIVPIAVNELIKFHRLPEAEEEE"
     gene            complement(8923..9954)
                     /gene="queA"
                     /locus_tag="IXT20_002385"
     CDS             complement(8923..9954)
                     /gene="queA"
                     /locus_tag="IXT20_002385"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HAQ4860810.1"
                     /translation="MLTTEDFDFDLPEELIAQTPLKDRDHSRLLVLDRKTGEMEDKHF
                     HEIIEELQPGDALVMNNTRVLPARLYGEKPDTGAHLEVLLLTNTQGDTWETLIKPAKR
                     AKVGTRISFGDGRLQAVVKEELEHGGRIIDFEYEGIFLEILESLGEMPLPPYIKERLE
                     DPDRYQTVYAKENGSAAAPTAGLHFTKELLDEIEAKGVELVYLTLHVGLGTFRPVSVD
                     KIEEHHMHSEFYRLTEEAAERLNQVRKNGGKIVAVGTTSIRTLETIGTKFNGEIKADS
                     GWTDIFITPGYEFKVVEAFSTNFHLPKSTLVMLVSAFAGRELTLSAYQHAIEERYRFF
                     SFGDAMFVK"
     gene            10147..>10740
                     /locus_tag="IXT20_002386"
     CDS             10147..>10740
                     /locus_tag="IXT20_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005232177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ4860811.1"
                     /translation="MIEFQNVSKIYKGGKVAVEDVNLSFEKGEFICFIGTSGSGKTTT
                     MRMINRMTDPTQGKILIDGKSIQEIDPVELRRQIGYVIQSIGLMPHMTIRENITLVQK
                     LLKVSQEERNKTAEKMIDLVELPREMLDRYPHELSGGQQQRIGVVRALAANQDIILMD
                     EPFGALDPITRDSLQDLVKDLQERLGKTIVFVTHDMDE"
BASE COUNT         2956 a         2311 c         1977 g         3496 t
ORIGIN      
        1 gttttccaag ttctttttca tctattttct tatctttttt ctacgctttc ttttgcttat
       61 ccgttgtaag catcaatgat gatctgtttc aattcagaga tcaatggttc tttcggatta
      121 gctgtcgtac attggtcttc gtaagctaat tcagccatac ggtctacggt tgtatctagt
      181 gtttcttgtg tgactccttg agcttttaga ctcatgttga tgccgacaga ttttcctaat
      241 tcataaacag ccgttgctaa agcttcgact aattcctctg ttgtttcgcc tttcagacct
      301 aagaattttg cgatatctgc gtaatctgta tctgcacgga aatagtcata tttagggaac
      361 atcgcatgtt tttgtggatc ttttgcatta tagcggataa tatgcggtaa aagaatcgca
      421 tttgtccgtc catgagggat cccgtattcc ccgccgattt tatgtgcgac tgagtgacaa
      481 atacctagga atgcgttagc aaacgccata ccagccattg tagaagcgtt gtgcattttt
      541 tcacgtgatt ccatatcagg tgtttttact gatttttcta aatagtcaaa gactaacttg
      601 attgcttgta ggctcaatcc acgtgtataa tctgaagcca tgactgaaac ataggattcg
      661 attgcatgtg ttaacacatc catcccggta tctgctgtga cagatgctgg aactgacatg
      721 acgaattgag gatcgacgat tgcgacatct ggcgtcaatg cataatctgc taacggatat
      781 ttcacatgtg tttcactgtc agtgatgacc gcaaatggcg ttacttcaga accagtccct
      841 gatgttgtcg ggatacatac gaattgtgtt ttttctggtt tgtcgatctt atatgtccgt
      901 ttacggatat ccaagaattt ttgttttgca ccaaagaaag atgtgtctgg gtgttcatag
      961 aacatccaca ttcccttagc tgcgtccatt gcagaaccgc ctccaagagc gattactgta
     1021 tctggcttga agtctgccat cagtttcgtt cctgcgtata cggtatttgt cgatgggttc
     1081 ggttcaacat ctgagaagac ttcgatcttc acatcatttt tacgtcgtgc caatacttca
     1141 cgaactgtat cgcagtaacc gaattggacc ataccagggt cacaaacgat catgacacgt
     1201 tcaacgtttt ccattttctc taagtacaat agagagttct tttcaaagaa gatttttggc
     1261 ggtaatttga accattgcat gttatttctc cgtttcgcta cagttttgac attgattaag
     1321 tttacagcag agacgttacg agaaacagag ttcttaccat aagaaccgca tcccaaagtc
     1381 aatgacggaa tcatttcgtt atagatatca ccgatgccgc cttctgcaga tggtgtattc
     1441 actaaaatac gacaagcttt cattcgaaga ccgaatttga catgcaactc ttcgttttct
     1501 gaatggataa ctgctgtatg tcctaatcct ccaagattca acataccttc acacaaatca
     1561 aacgcatggt ccgtgttgtt agctttgatc atggcaagaa ctggagaaag tttttctctt
     1621 gatagcggat aatctgggcc tacgccttcc aattcagcaa tcagcatttt cgttcctttt
     1681 ggtacactga ttcctgccaa ttttgcgatt tccatcgcag aatgtccaac gattgatgga
     1741 ttgaccgcat attttccttc gttcatcact gcatcttcta atttttgcaa ttcatctggt
     1801 tttacgatat aaacttggtg tgcttggaat tctgctttta ctgctgcata gatttcttta
     1861 tccacgatca cagcttgttc agaagcacaa atcattccat tatcaaatgt tttcgaaacg
     1921 atcaaatcgt tcactgcacg tttaattttt gctgtttttt caatataagc tggtacgtta
     1981 cctggaccta cacctaaagc tggttttcct gtagagtaag cagacttgac cattccagct
     2041 ccacctgtcg ctaagacgat cgcgatacct ggatggttca ttagcataga agtcgcttcg
     2101 atcgaaggat gttcgatcca ttggatacaa ttttcaggag ctccggcagc gatagcagca
     2161 tcacgtacca ctcgtgctgc ttctgcagaa gatttttggg cacttggatg gaaagcaaaa
     2221 acgattggat ttcttgtttt caacgcgatc attgatttga aaatcgttgt ggaagtcgga
     2281 ttcgttgttg gagttactcc gcaaaccaca ccgactggtt cagcaatttc aatcaaccct
     2341 ttttggacat cttcattgat cacaccaact gttttatcat gtttgatatt atgccaaata
     2401 tattcggacg catacatatt tttgattgct ttatcttcgt aaacaccgcg gcctgtttcc
     2461 tctacagcca tcttcgctaa tggcatatgt tgatccaaag ctgccattgc catttcatgc
     2521 acgatatggt ctactttttc ttgatcaaag ctttccattt ctttcaatgc tgcatttgct
     2581 ttttctgcca aatcatcaat cattgcttgt acgtcaattg tcttcgtttt ttctttttct
     2641 attactttag ccatcattat cctcctaatg ggtcatggtt atttgttaaa taattcacat
     2701 gtatatcata caatatttat ttttctatgt aaacactttt ttgtgatttt tttcacagtt
     2761 atttttaaat ataaaattgc tttatttaca ggtgtttgta tagaaaaata tataaagtta
     2821 tttattatta tttatatgac acacccgatt attcaatatt gagcaatata tcccatgctc
     2881 ttatctcttt cttttttgaa taaaaaatga ggtaggacat ctttctgccc tacctcgtct
     2941 ttaaataagc tttgatactt atttttcttc cttctcttct gtctcttcca gcgtttctgt
     3001 tgtttcagtt tttacttcaa ctgtctcagc tggttcttct gttgtttcaa cagtagtcgt
     3061 tgcatcattt gttacgacag tccctggttt gaccgttttg attgctgcac gatcgtactc
     3121 aaggaagatc ccttcgcagt ctaaaacaac tgtccgtttg tcattgtcga tttctgaaac
     3181 aactccatgt aatccgccga tcgttacgac tgaatcacct ggtttcatgc tatctaacaa
     3241 gttttgacgt tcttgctgtt gtttcttttg cgaacgtgac ataaaaaacc acattgctaa
     3301 tagcgcgata aacatcacaa gcattggtaa tgttcccatt tttttcacct cactaattct
     3361 gtatctacaa aaaacacttt agcagaaaaa tgctgataac actagttttt tacttaattt
     3421 ttaaaagatt ttcctttaga agctttttgc gttttcttta ttgaacccgt attcttcaaa
     3481 aaaggcttct ctaaattcaa gcaaattatc atccatgatt gcttgacgta cttgtttcat
     3541 tagattcaac aagaagtata aattgtggta agaagtcaaa cgaatcccaa atgtttcatc
     3601 acatttgatc aaatggcgga tataggcacg tgtatagttt ttacatgtat aacaatcaca
     3661 tttctcatca attggacgga aatcatgggc atatttcgca tttttcacta ctagacggcc
     3721 ttgagaagtc atacatgtgc catttcgagc gattcgagta ggcaagacac agtcaaacat
     3781 gtctactcca cggataacac catcgatcaa tgaatcagca gcaccgacac ccatcaagta
     3841 acgtggtttg ttttcgggga tcaacggtgt agtgaattct aatacacgat tcatttctga
     3901 tttaggttcc ccgacagata acccgccgat tgagtaacca gggaaatcca tcccgactag
     3961 atctttcgcg ctttgacgac gaagatcttc aaaccctgct ccttggatga ttccaaataa
     4021 tccttgacgg tctggattag catgagcttt caatcctcgt tctgcccatc gagacgtacg
     4081 ttctactgat tttttcacgt agtcgtaact ttcatcgaat ggtggacatt cgtcaaaact
     4141 catcatgata tcagagccca atttattttg gatgttgatt gctttttctg gtgataaaaa
     4201 catacgcgaa ccgttcaagt gatttttgaa atgcacgcct tcttcttcga tattccgcat
     4261 atcacttaat gaaaaaactt gaaagccgcc tgaatctgtt aaaatcggtt gatcccagtt
     4321 cataaattta tgcagtccgc cagcttcttc tactaagtct tcaccagggc gtagccataa
     4381 atgatatgta ttacttaaaa taacgccagc acccatttct ttcaactctt ctggggacat
     4441 tgtttttact gtagctaatg ttcctactgg catgaacatt ggtgtgggga aagttccgtg
     4501 tggcgtgatc aattccccta aacgcgcgcc agtatgtttt tctttcttga ttaaacgata
     4561 gcgaatggca ggttgcgtca ttcatttccc tactttctat ataaaatatc ttgacaactc
     4621 ccaccatcat aatcgtttca agtgcttttt gcaagttccc tcggcttcct tctactttat
     4681 ttaatattct tttttttatc ctttttttat gaataacaaa aaaaagaaaa acattatgac
     4741 agaaatattt tttgatacat ggtatattgg tgttattaaa gaaaaggagg gaaaaatcat
     4801 gcaatcttct gcagaattaa aacgccaagc aaaagagagt ctaaaaggac gttggggaca
     4861 agctgtttta ctaaatctaa tccctacatt gattggtatt gctactattc ttcttattat
     4921 gattcctatt atcttgtttg ccaccaccat atatgatgga tcagcgagcg tctcagaagc
     4981 gacaacttca ggaggcggta gttcaggagg cggtggcgga attatttcca gcatcattag
     5041 cgctttgttc atgagcggta tttcttggac ttatcttgat attttacgtg gacaaaaaga
     5101 tcagatcgaa ccattcaaag atgcatttcg tggattcaaa ggtttgttta tcggcggagt
     5161 gatcagctta gctgtgctga tctcgatctt tacaacatta tggggtttgc tattagttat
     5221 tcctggaatc attaaaagct attcttattc tcaaagctat tttatttact acgatatcgt
     5281 ccaagaaaca ggacaaacac ctaaagtact ggatacgatc actgcaagtc gaaaattgat
     5341 ggatggttat aaaggacaac ttttctggtt agatctttca tttatcggct ggcatatctt
     5401 agcattagca actgctggta ttggttatct atggctcagc ccatacattt ctgcaacaaa
     5461 agcagcattt tatgaacaat tgccaaaaga tatttaaaag agagtgtgac ttgtgaccga
     5521 gggggtggca ggagaacacc gtttatcgag aggcagtaaa agaaacattc agcccattag
     5581 caaaaaggag gtgtgacaac attcatgtca cacctccttt ttgtatagtt ccagtaaaaa
     5641 gaaaatcaac ttagaaacag ttaatcattc ctcttcttct gcttccggta atcgatggaa
     5701 cttgatcagt tcattcaccg cgattggtac gatagataaa atccctacca agaaccagtg
     5761 attcaaactg atgtgtacta ggccgaatag ttcttgcgta aatggcagca aagcaacagc
     5821 cgttgtaata gctaatgcca gtaacgccat ttctaccaaa gctttattgg tcgctaaatt
     5881 gactttgaag atcgattggc tgctgcgtac attgaacaca tgcagaatcg atgaataagc
     5941 taagatcaag aatgcaacag tttgaccgac atgctgactt gcgtctaccc atgggctgat
     6001 agtatcgaca taactaccta aataataacc aaacaaggtt acgatggtga agacccctgc
     6061 atttatggca attttttgca ataaacctcg agcaaagatc ccttcatttt tcggaatggg
     6121 tggctgatcc atgatgccgt gttccgctgg ctctcttcct aaagcaaatc ctggaagtcc
     6181 atcagcaact acattgacaa acagtagttg cacagctgta aatggtgctc cccatcctaa
     6241 cagcatcgca atcaaaacaa taaagatctc cgaaatatta cagcttagca aaaagttgat
     6301 agctttacga atattctgat aaaccgaacg tccttgactg acagcgtcta cgatcgtcgc
     6361 aaagttgtca tccgtcaaaa tcatatctgc ggcactttgc gccacatctg ttcctgtgat
     6421 ccccatggca cacccaacgt tacttgcttt caacgctggt gcatcattca caccatcccc
     6481 agtcatcgct acgactgcac ccgtacgttg ccaagatttg acgatccgga tcttgtcttc
     6541 tggcgttacc cgagcataga cagagagagc tttgacacgc gcatctaatt cttcgtctga
     6601 taattgatgc agttctgatc ctgaaagtgc ttctgacgga tcattcaaga tacctaattc
     6661 tttcgctatg gcactggcag tcaccacatg atctccagtg atcatgactg ttttgatgcc
     6721 agcctttttc gctcgcttga ttgctccttt actttctgga cgtggtggat cgatcattcc
     6781 gatcagacca atcaaacgta agtctttttc cagtgcttca gaagaaatat cttgcggttc
     6841 ttcgtcataa gtagcataac caacagcaat cactctcagt gcccgtttac caaagcgatc
     6901 attaacgatc gccgcttgtt ctacatcgcc tgaactaaag cgcggagcaa gaacatcgaa
     6961 tgctcctttg gtgattgaga tgtatttttc gcccatttgg tgaactgttg tcatcatttt
     7021 tcgttcagaa tcaaaaggaa gctcgtttac tcgtggatat ttttcttcta attctgcctt
     7081 tgtatgataa ttctcttcaa cagcccttac gatggctact tctgttggat tacctttgat
     7141 aacaaggtcg tcatcttctt gctcgaccgt cacatctgta catagtgcag ccattttcaa
     7201 tatttccatc gcgctgttcg tcattgaatc ttcgatattc gttacttcat cttctttgct
     7261 ccatacccgg cggacgcgca tttcattttg tgtcagcgtt cctgttttgt ctgagcagat
     7321 aacattagcg gttcctagcg tttcaactgc tggcaactgt cggatgatcg catgtttcct
     7381 cgccatcttc tgcacaccgt atgcaagtgt caacgtaaca ataacagtca aggtttccgg
     7441 tacagctgca acagccaagg aaatcgaagt catgaacatc tcaaggagtg gttctccttg
     7501 cagttcacca atcacaaata ctaaagccgc agccgctaaa gcgatcatac tgattcgttt
     7561 gcctagctga ttcaatcgga cctgtaaagg tgttttttgc atagtatttt cgttcaaaag
     7621 atccgcgatc ttacccattt ctgtagccat gccaatggca gtgacgatcg ctttcccacg
     7681 acctgcagca accgtacatc ctttaaatac catattaagt tggtcaccta atgactcatc
     7741 ttcttcagca ataaatgctg catctttttc aactgcttca ctttctccag tcaacgccga
     7801 ttcttctaca cgtaattgtg atgcttcgat caaacgggca tcggcagaaa tagcatcacc
     7861 ggactctaaa acaagaatat cacccactac cagttgctct gactcaacag tggtcacttt
     7921 gccgtctcgt atgacagttg ccgtttgttt attcatatct tgcaaggatt ccagtgcttt
     7981 ttctgcgttt ccttcttgga caatcgccaa taccgagttg atcacaacga tagcgatgat
     8041 cagcagtcct tcaaataaat ccccatgttc tgtagcaaat gcggtataaa aggaaatcac
     8101 cgctgctaca agaaggatga tcgttgtaaa atcggacaag cttcgaaaga attttttgat
     8161 catcgattct ttcggcgcct catcgaattt attcaatccc tgcttttttc gccgttcatc
     8221 cacttcttgt gaagacaacc cagtatctgg atctgcttct gttaccttct ttacttcttc
     8281 tattgtctgc ttgtattctt ccatcctatt cctcctttaa aatcttcgta caaaaaaagg
     8341 tacttatttt actataacat ccttttttta tttacttcaa ataaagggct tatcgattat
     8401 ttgttaagaa agcgtaaaat ttaaagtgct tatatttgca aacgctacca aaggatgcta
     8461 ttatatagat gtaaggaagg aggataccat caaatagaca gaagaatttc tcgatagaac
     8521 agtgtaagca aacaaagttg ttagaagtac aggtttcacc cagacatgcg tgtagagtac
     8581 aaacctataa atagtgaatg cgtatggatg tttggatcac gaagtatccg aaacaagaag
     8641 taatgcgata cgtaacaatt gaataacgta aacgtctgaa aattgaatag gaacaagaga
     8701 tgtttcaaat gttgaagatg taacgaaaac aaaatttttg tttaacatga agaaattcaa
     8761 caccgtgctt tgcatgtatg atggaaagtt acaatcattt ctgtttattt gaatccttcc
     8821 gaggtacaag aaagccgagc ttatcgagag ctcggctttt tctttgtcat aaaatctttg
     8881 taaagctttt ttgttattca aaactagcgg cttttcataa ccttatttca cgaacatcgc
     8941 atctccaaaa ctaaagaaac gatagcgctc ttcaatcgca tgctggtaag cagataaagt
     9001 caattctctt cctgcaaagg cactaactaa catgactaaa gtcgattttg gcaaatggaa
     9061 attagttgag aaagcttcta ccactttgaa ttcatagcca ggcgtaatga aaatatcagt
     9121 ccatccgctg tctgctttga tttctccatt aaatttcgtt ccaatcgttt ctaatgttcg
     9181 aattgaagtt gttccgacag ccacgatttt cccgccattt ttacgtactt ggttcaatcg
     9241 ctcagccgct tcttccgtca aacggtagaa ttcactatgc atatggtgtt cttcaatttt
     9301 atctacgctg acaggtcgga atgtacctaa ccctacatgt agtgtcagat agactaattc
     9361 aacacccttt gcttcgatct catcaagtaa ttctttcgta aaatgcaatc cagctgttgg
     9421 cgctgcagca gaaccatttt ctttcgcata gacagtttga tagcgatctg ggtcttccag
     9481 acgttctttg atatatggag gcaatggcat ttctcccaat gattcaagaa tctctagaaa
     9541 aattccttca tactcaaagt caatgatccg tccgccgtgt tccaattctt ctttcactac
     9601 tgcttgcaat ctgccatcac caaaagagat tcttgtccca actttggcac gtttcgcagg
     9661 tttgatcaat gtctcccacg tatctccttg tgtattcgtt agaagaagga cttctaaatg
     9721 cgcaccagta tctggttttt ctccatataa acgagcaggc agaactcgtg tattattcat
     9781 aactagcgca tcaccaggct gtaactcttc aatgatttca tggaaatgtt tatcttccat
     9841 ctctccagtt ttccgatcca aaacaagtaa acgagaatga tcgcggtctt ttaatggtgt
     9901 ttgtgcgatt aattcttccg gcaagtcaaa atcaaaatct tctgttgtta acatatcgtc
     9961 actcactttc tatcatgtgc ttcatcattc tatcatgttt ttcgttctgt tggcaaaatc
    10021 agcacttttt cacttctatt ttttgttttc ctaaataaaa aaactaccct atttgtattt
    10081 tttcattgtt ttaggcatac tagtaataga tagttcttca ttgaaaatag aaaaagaggt
    10141 ggcaccatga ttgaatttca aaatgtgtca aaaatttata aaggtggaaa agtcgctgta
    10201 gaggacgtca atctttcatt tgaaaagggt gagttcattt gttttatcgg tacgagcgga
    10261 agcggaaaaa cgacgacgat gcggatgatc aaccgaatga ctgatccaac acaagggaaa
    10321 attttgattg atggaaaatc catccaagaa attgatccag tggaattgcg acgtcagatc
    10381 ggctatgtta tccaaagtat tggattgatg cctcacatga cgatccgtga aaatatcact
    10441 cttgtccaaa aattgttgaa agtcagtcaa gaagaacgga ataaaacagc tgaaaaaatg
    10501 attgatcttg tggaattgcc ccgtgagatg ttagatcgct acccacatga attatctggt
    10561 ggtcagcagc agcgtattgg agtcgttcgt gcccttgcag cgaatcaaga tatcatcttg
    10621 atggacgagc cattcggtgc gcttgatcca atcactcgtg attccttgca agaccttgtc
    10681 aaagatttac aagagcgttt gggaaaaacg atcgtatttg taacccatga catggatgaa
//
LOCUS       DACGLE010000086        10617 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0534
            SAMEA3304449-rid9560453.denovo.091, whole genome shotgun sequence.
ACCESSION   DACGLE010000086 DACGLE010000000
VERSION     DACGLE010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304449
            Sequence Read Archive: ERR985064
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10617)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10617)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702225.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702225.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 20:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,045
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 27 of 67
            Pseudo Genes (incomplete)         :: 25 of 67
            Pseudo Genes (internal stop)      :: 19 of 67
            Pseudo Genes (multiple problems)  :: 4 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10617
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304449-rid9560453.denovo.091"
                     /strain="VREN0534"
                     /db_xref="taxon:1352"
     gene            290..2236
                     /locus_tag="IXT20_002387"
     CDS             290..2236
                     /locus_tag="IXT20_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIA"
                     /protein_id="HAQ4860812.1"
                     /translation="MIERHVNLLKLLSRQEDFKPASFFSDKLAVSTKTIYQDIELLED
                     YVVNYQVTIERLPSLGIKLIGDRKNVEHLISSVENYLEKDIYSPEKRRVEIIKEVLFG
                     DKPVSLTHLSQSFMVSKTSLYNDLRIINKIIGSGEVKLQSSAEGISFTGNEQAIQRAI
                     KQLVFYYANDMESKTFFQVLNMLFDSKVISVIHDLLFNDYSELTEKVSDYYVRSLIST
                     LVIQCNRLLKGHHLTVEEEFLFNSIRYMETYIVANGLIEELKAKLNLPFYDADKEYLC
                     RQLFAHRVTNNLKTSNEEYAMSVHKLIERMSEIEKIDLTQNDRLYQSLLYHVPAMVLR
                     LKKGIKIQNPLLENIKSQYSELFSIVWYAMSILEEEFDVTLTDDEVSLILIHFQIAIE
                     DQSKANNIIIICQYGISSAQLIYNKVRRFLPAKDNVEISTLDKFHLAEIGEVDLIISS
                     LSFDAGGIPLVRVSPLVSKTDYMNIMEAYTKYLIEEPSRFIQNELIPKAVKVPTISKF
                     VQSSLVKTGVQVESKEECLDLMISELEANSLVTEEFRASIFSREKMGNTNLESGVALP
                     HANPSMVIESNIFIVALKNAINWGDRKVKLVIMVSLAEEDVSEIRGVYEELYQFIGQK
                     EMVDRLLQVHDSEKLLRIFQQKGE"
     gene            2239..2694
                     /locus_tag="IXT20_002388"
     CDS             2239..2694
                     /locus_tag="IXT20_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAQ4860813.1"
                     /translation="MYLDKELCLFQLEVKDQKELFQVMSEQLKNAGCVKDSFLEGITN
                     REQEFPTGLEVNQIGFAIPHTDSAHVNSSQICFASLKEPLVFSDMTDKSHEIPVRLVF
                     MLAMSQPHEQIDTLQNLVSLFQNEEKVNELLACTTKEAFIELVHSAGVY"
     gene            2708..4036
                     /locus_tag="IXT20_002389"
     CDS             2708..4036
                     /locus_tag="IXT20_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HAQ4860814.1"
                     /translation="MDIVLNAFEWFIGLGSNVFLPLIIFVIGILFGLKPGKAFVSGVT
                     VGIGSIGLGLVLSLLSDGLGTAIQQMGEQYGGGLNILDIGVGVGGPLAYSTSLGILMI
                     PISLILNFIFVVLGWTKTLNVDIWNFWFAIFLGMITQSVTGNFWFGLIGAIVAVILQW
                     FLADRTQKTVSEFFGYPGISITHMMGLSGVLFAKPVNWVFDRIPGFNKIDADAETLTK
                     RFGVLGDTVVIGLIIGIVVGIMAGYDVAGIGNLGIMTAAVMKIMPKMVAMFMEGLMPI
                     AEAAKEFTNKKLNGRQVNIGMDAALTVGHPTIMSTSLLLVPISLLLALILPGNKVLPF
                     GDLAFFAFAICLMIPYFKGNIVRSLVGCSLYVVTTLYMATWLAPIITDVFTLASFDVG
                     TAGLVTMLLCGLWPVGLFVLLASYFGVIGIGGLGVVLVGASIYMNKVKKA"
     gene            4070..4354
                     /locus_tag="IXT20_002390"
     CDS             4070..4354
                     /locus_tag="IXT20_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ4860815.1"
                     /translation="MKKLLVICGAGHATSTIAVAKLSKWLEEEGLTDQVKIYQSKIAD
                     ELNKIDEYDAVVSTTIVPDVIKAKVTNGLGLLTGIGTDAIYADVKERLGV"
     gene            4356..4871
                     /locus_tag="IXT20_002391"
     CDS             4356..4871
                     /locus_tag="IXT20_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbQ family protein"
                     /protein_id="HAQ4860816.1"
                     /translation="MRFHHDTLTIETVSGRPSYHDIRKELLDTVDTAGIENGMLVISS
                     PHTTCSLFFEETMHDTNFFGDDYLHVDINNVMETIIPKMTSENQYNSPGPKHIDFGTR
                     LSDPNYPAEKWVMLNTDAHLRSSIYGTNSMTLIVQEGKLLLGEMGRVYFVDWDQLRER
                     TRTINLMIMGE"
     gene            4887..5549
                     /gene="rpe"
                     /locus_tag="IXT20_002392"
     CDS             4887..5549
                     /gene="rpe"
                     /locus_tag="IXT20_002392"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HAQ4860817.1"
                     /translation="MTIAPSLLNAPIAEVKELFEILKEKNVDYLHIDVMDGCFVPDMA
                     FGPSVIKDIRPLTDVVFDVHLMIDTPEKIVTRFVEAGADIVTFHIEATKHAMKVIQLV
                     KEAGAKVGITFNPATPVSFIEPVLSLVDQVLVMTINPGTENKHFIQETVVKIEQLDVI
                     RKQNDYTYDIEVDGKIDNQTIKVCSKAGADIFVSGGFIFNGKNTPQEQIDKLNQSLEE
                     VK"
     gene            5556..6128
                     /locus_tag="IXT20_002393"
     CDS             5556..6128
                     /locus_tag="IXT20_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAQ4860818.1"
                     /translation="MTNKDQNKNYLEAIILELQNNSKRINSVELEQFSEKIVQAKHIF
                     LAGAGRSGIAMQAFTNRLMHLGFSVSLVGEISSPHSQPGDLLIICSGSGETGSLKNLA
                     AKANQSGVSIALVTMKKDSSIGQLADSVLVLPGITKDENQREEDTFSQPMGSSFEQLA
                     FLTFDSLVLSLMDKTGESSETMFARHADFE"
     gene            complement(6315..7835)
                     /locus_tag="IXT20_002394"
     CDS             complement(6315..7835)
                     /locus_tag="IXT20_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HAQ4860819.1"
                     /translation="MTKMESELLVQIKQLTQLLEIQQQENTLLREQIAEMNRRLFGRK
                     KETPPVDGQIDLLDDSTFNEPEHTGQESQEPITVSSFKRRKRKGLKALSLEGLPEVPI
                     HYELKGADCLCEDCGHTLHDIGATRLREEPLYVPAHIEKQVIYQHAYHCRECEALGET
                     KIKKAPVPKPLINNSLGSSSIVTQSIWEKFVKKVPAYRQEKEWHALGFPLDRKKITNW
                     HIKVSEMVLEPIYNCLHQELSNQEVLHADETSYRVLNTHKEKTFYWLYASGKSEEKQV
                     VLYEHANSRSVDEPKSFLESFSGFLHTDGYAAYGKLPVNNVYCWAHVRRKFFEALGKQ
                     PSPKSHAKIGLDFCDKMFRIERTFNKLSAEERFVARKETLQPVMTEFFHWCESFAYLP
                     KSQLGKAVQYAVNHKDGLQIVLLDGRLELSNNRAERAIKELVIGRKNWLFSKSLKGAR
                     SNGIILSIIQTAVANGLNIRKYLNHLFTEIPNLSSMTPEALRAYLPWNQQIQEICK"
     gene            complement(7931..8056)
                     /locus_tag="IXT20_002395"
     CDS             complement(7931..8056)
                     /locus_tag="IXT20_002395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAQ4860820.1"
                     /translation="MYKRFESGYLQWPRSRKEVLLTGLSIEEKCTLKETKKGAIY"
     gene            complement(8078..8263)
                     /locus_tag="IXT20_002396"
     CDS             complement(8078..8263)
                     /locus_tag="IXT20_002396"
                     /inference="COORDINATES: protein motif:HMM:NF017528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence hypothetical
                     protein"
                     /protein_id="HAQ4860821.1"
                     /translation="MPMLIDATALKHIFIVCGKTDLRKGIDGLAAFVIEEYDLDVYDQ
                     ALFLFCGTKKDRFKALY"
     gene            complement(8247..8429)
                     /locus_tag="IXT20_002397"
     CDS             complement(8247..8429)
                     /locus_tag="IXT20_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4860822.1"
                     /translation="MNKKDIVPVQFSSAAQKSQIDTKPKLACRIKSEDKEIFIYNGMD
                     KYLLYTLLKELNANAH"
     gene            8670..8888
                     /locus_tag="IXT20_002398"
                     /pseudo
     CDS             8670..8888
                     /locus_tag="IXT20_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997693.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
     gene            9383..9982
                     /locus_tag="IXT20_002399"
     CDS             9383..9982
                     /locus_tag="IXT20_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAQ4860823.1"
                     /translation="MKTVEPIRDKKKIDAMKAILASGKYGQRNLVLFSIGINTAYRIS
                     DLRQLKLADVLEISRGRVVVKDRLAMKEQKTAKHNSVYISNKLRKTILEYVQQEFPDQ
                     LLQQDFDGYLFPSRKGTNEPLNRQSLWRIIHDAGTAIGLKDIGPHSMRKTFGYFLYKQ
                     GTKTEIIQSLLNHSSQRETLRYIGITQEDKDTAVKSLDL"
     gene            10046..>10617
                     /locus_tag="IXT20_002400"
     CDS             10046..>10617
                     /locus_tag="IXT20_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4860824.1"
                     /translation="MSTLYIIGNGFDLSHGLKTRYTDFSKWVEKSHSEIFDKIDTMIN
                     ENNEVRSTADDKIGWFNFESSLHELIQSPVFEDYVEESKDYLIGYGHEDWSDSAHHDF
                     QNMVYDYVVSLEELQQLFKEWIRKEIQPTIEHKIPKYQFSSDVLFFTFNYTDVLEILY
                     KQPKENIYHIHGSYDDIIIGHSENFDNFIG"
BASE COUNT         3512 a         1670 c         1981 g         3454 t
ORIGIN      
        1 attataataa aaaaatacga attttagtga cgccattata ttgtttttat ttttcaattg
       61 taatatttca tattacttcc ataataagga gtattttcta ttggaatatc ttagctcttt
      121 taaggatttc tccttttttg tttctcattt tttaagatta tttggatttc ctttacaaca
      181 aacttccaca agtattattg acatagatgt aattgaggga agggggttcc agtgctagaa
      241 taaataatga aaagaggtaa caaatgctag cgttaagagg tgaacaagtt tgattgaaag
      301 acatgtaaat ttattaaaat tattaagtcg acaagaagac tttaaacccg cttctttttt
      361 tagtgataaa ttggcagtgt cgaccaagac catttatcaa gatattgaat tgttggaaga
      421 ttatgtagtt aattaccaag taacaattga gcgattacct tctttgggta tcaaactcat
      481 aggtgatagg aaaaacgttg aacatttgat ttcatcggta gaaaattatc ttgaaaaaga
      541 tatttattct ccagaaaaaa gacgtgtcga gatcattaaa gaagttttgt ttggtgataa
      601 gcctgtttct ttaactcatt tgtctcaaag ttttatggtt agtaaaacat cgttatacaa
      661 tgacctgaga attatcaata aaattattgg cagtggagaa gttaagttgc agtcatctgc
      721 agaaggaatt agttttactg gtaatgaaca agcaattcaa cgagcaatca agcaacttgt
      781 attctattat gcgaatgaca tggaatcaaa aacatttttt caagttttaa atatgttgtt
      841 tgattcaaaa gttattagtg tcattcatga tttgctattc aatgattaca gtgaactgac
      901 tgaaaaggtt tctgattact atgttcggtc attgatttct actttagtta tccaatgtaa
      961 ccgtttactg aaaggtcatc atttaactgt tgaagaagaa ttcctcttca atagcattcg
     1021 ctatatggaa acgtacattg tggcaaatgg tttaattgaa gaattgaagg caaaattgaa
     1081 tcttccattt tatgacgcag ataaagaata tctatgtcgt caactatttg cacatcgtgt
     1141 gacaaataat ttaaaaacca gcaatgaaga gtacgctatg agcgttcata aattgattga
     1201 aagaatgagt gagattgaaa aaattgattt gactcaaaat gatcgattat atcaatcctt
     1261 attgtatcat gtgccagcaa tggtccttcg tttaaaaaaa ggtattaaaa ttcaaaatcc
     1321 gttattagaa aatatcaaat ctcaatattc agaactcttt agcatagtat ggtacgccat
     1381 gtccatcctt gaagaagaat ttgatgtgac tttaacggac gatgaagttt ccttaatttt
     1441 gattcatttt cagattgcta ttgaagatca gtccaaagca aataatatca tcattatttg
     1501 ccaatacggt atttcttctg cgcaattaat ttacaataaa gttcgacggt ttttaccagc
     1561 aaaagataat gtagaaatat caactttaga taagtttcat ttagctgaaa ttggggaagt
     1621 tgatttgatt atttcctctc tttcctttga tgcaggtggt atacccttag taagggtatc
     1681 tccattggta agtaaaacag attatatgaa tattatggaa gcttatacta aatatttaat
     1741 agaagaacct tcgcgattta ttcaaaatga actgattcct aaagcggtta aagttcccac
     1801 catatctaaa tttgttcaat catctttagt aaaaacagga gtacaggtgg aaagcaaaga
     1861 agaatgtttg gatttgatga tttccgaact agaagccaat tcattagtta ctgaagagtt
     1921 tcgtgcttct atcttctcta gagagaaaat ggggaatact aatttagaaa gtggtgttgc
     1981 tttaccgcat gcgaatccat caatggtcat tgaatccaac atttttattg tagcattaaa
     2041 aaatgcgatt aattggggcg atcgaaaagt caaattagtc attatggtta gtttagctga
     2101 agaagatgta tctgagatta gaggagtata tgaagaactt taccaattta ttgggcaaaa
     2161 agaaatggtc gatcgcttgt tacaagttca tgatagcgaa aaattactaa gaatttttca
     2221 acaaaaagga gaataaaaat gtatctagat aaagaattat gtttatttca attagaagtg
     2281 aaagatcaaa aagaattgtt tcaagtaatg tctgaacaat taaaaaacgc tggttgcgtg
     2341 aaagatagtt tcttagaagg tatcaccaac cgtgaacaag aatttccaac tggattagaa
     2401 gtaaatcaaa ttgggtttgc tatcccacat acagattccg cacatgtaaa tagctctcaa
     2461 atctgttttg cctcattaaa ggaaccactg gtattttcag atatgacaga taaatctcat
     2521 gaaatacctg tacgtttagt ctttatgttg gcaatgtcac aacctcatga acaaattgat
     2581 acactacaaa acttagttag cctatttcaa aatgaagaaa aagtaaatga attacttgca
     2641 tgtaccacga aagaagcatt tattgagtta gtacattctg caggagttta ttaggaggaa
     2701 tagagagatg gatattgtat taaatgcctt tgaatggttt attggattag ggtcaaacgt
     2761 ctttttacca ctgattattt ttgtaatcgg gatattattt ggattaaaac ctggtaaagc
     2821 atttgtttcc ggcgttactg ttggtatcgg ttctattggt ttaggattag ttttaagttt
     2881 attatcagat ggtttaggaa cagcaattca acaaatgggg gaacaatacg gtggtggcct
     2941 gaatatccta gatattggcg taggtgttgg tggacctttg gcttactcta catcattggg
     3001 aattttaatg attccaattt cattaattct taacttcata tttgtggttt tgggttggac
     3061 aaaaacattg aatgttgata tttggaactt ttggttcgca attttcctag gtatgattac
     3121 tcaatctgtt acgggaaact tttggtttgg cttaattggc gcgattgtcg cagtaatttt
     3181 acaatggttc ttagcagatc gtacacaaaa aactgttagc gaatttttcg gctatccagg
     3241 aatctctatc acacatatga tgggattatc aggagtacta tttgccaaac cagtaaattg
     3301 ggtttttgat cgtattcctg gatttaataa aatagatgca gatgcagaaa cattaacaaa
     3361 acgtttcggt gttttaggtg acacagttgt tattggccta attatcggta ttgtagtggg
     3421 tattatggca ggctatgacg tagcaggaat tggaaactta ggaattatga ctgcggcagt
     3481 gatgaagatt atgcctaaaa tggtagcaat gtttatggaa ggtttaatgc caattgcaga
     3541 agcggcgaaa gaatttacga ataaaaagtt aaatggtcgc caagtaaaca ttggaatgga
     3601 tgcagcgtta actgtgggtc atccaactat tatgtcaact tctctattgc ttgtaccaat
     3661 ctcattattg ctagcactaa ttcttccagg aaataaagta ttaccttttg gagatttagc
     3721 attctttgct ttcgccatct gtttaatgat tccttatttt aaaggaaata ttgtgagatc
     3781 attagtagga tgttcgttgt atgttgttac aacattatat atggcaacat ggttggcacc
     3841 aattatcact gatgtgttta cattagcaag tttcgatgtg gggacagcag gattagtaac
     3901 tatgttgtta tgtggattat ggccagtagg gttgtttgtt ttattagcat cttactttgg
     3961 tgtaattgga ataggtggat taggagttgt tttagtaggt gcttctatct acatgaacaa
     4021 agtgaagaaa gcttaatcat aaaattaaaa taagaaagaa gagatcaata tgaaaaaatt
     4081 attagttatt tgtggagcag gtcatgctac ttctacaatt gcagtagcaa aattatcaaa
     4141 atggttggaa gaagaaggat taacagatca agtgaaaatt tatcaatcta aaattgctga
     4201 tgagctaaat aaaattgatg aatatgatgc tgtagtaagt acaacaattg tccctgatgt
     4261 aattaaagct aaagtaacga atggcttggg cttattaaca ggtattggta cagatgccat
     4321 ttatgcagat gtaaaagaaa gattaggtgt ttaatatgcg ttttcatcat gacactttga
     4381 ctattgaaac agtttcaggg cgtccttcgt atcatgatat tcgaaaagaa cttttagata
     4441 cagttgatac agcaggtatt gagaatggaa tgttagtaat ttcgagcccg catacaactt
     4501 gctcactctt ttttgaagaa acgatgcatg acacaaactt ttttggtgat gattatttac
     4561 atgtagacat caacaatgta atggaaacga ttattccaaa aatgacgagt gaaaatcaat
     4621 acaacagtcc ggggccaaaa cacattgatt ttggaacaag attatctgat cccaattacc
     4681 ctgcagaaaa atgggtcatg ctaaataccg atgcacattt acgttcatct atttatggaa
     4741 ccaactcaat gactttaatt gttcaagaag gtaaactttt attaggagaa atgggccgtg
     4801 tctattttgt cgattgggat caactaagag agcgaactag aacaatcaat ctcatgatta
     4861 tgggagaata aacgggggaa tagagaatga caattgctcc atcattgtta aatgcaccta
     4921 tcgcagaagt taaagagtta tttgaaatct taaaagaaaa gaatgttgat tatttgcata
     4981 ttgatgtaat ggatggctgt tttgtaccag atatggcttt tggaccaagt gttattaaag
     5041 atattcgtcc actaacagac gttgtgtttg atgttcattt gatgattgat acaccagaaa
     5101 aaattgtcac aagatttgta gaagcaggag cagatattgt taccttccac attgaagcta
     5161 ccaaacacgc aatgaaggtc attcagttag tcaaggaagc aggagcaaaa gtagggatta
     5221 cttttaatcc tgcaacacca gtcagtttta ttgaaccagt attatcttta gtcgatcaag
     5281 tattagtaat gacaatcaat ccaggaacag agaataagca ttttattcaa gagacagtag
     5341 ttaaaatcga acagttagat gtaatacgca aacaaaatga ttatacgtat gatattgaag
     5401 tagatggcaa gattgataat caaacgatta aagtttgttc taaagcaggg gcggatattt
     5461 ttgtatcagg cggtttcatc ttcaatggga aaaatacccc gcaagaacaa attgataaac
     5521 taaatcaatc attagaggaa gtgaagtaaa agatcatgac caataaagac caaaataaaa
     5581 actatttaga agcgattatc ttagaattac aaaataactc aaaacgtatt aattctgtag
     5641 agttagaaca atttagtgag aaaattgttc aagctaaaca catctttcta gctggtgcag
     5701 gacgctctgg aattgccatg caagcattta cgaatcgctt aatgcattta ggtttttcag
     5761 tcagccttgt cggtgaaatt agttcaccac attcgcaacc tggagattta ttaattatct
     5821 gttctggctc aggtgaaaca ggtagtttaa aaaatttagc ggccaaagca aatcaaagtg
     5881 gtgtaagcat tgctttagtt accatgaaaa aagactcgtc gattggtcaa ttagcagatt
     5941 ctgttcttgt attaccaggt attaccaaag atgaaaatca acgagaagaa gatacttttt
     6001 cacaacctat gggttcaagt tttgaacaat tagcatttct cacctttgat agtttagtgt
     6061 tatctttaat ggataaaaca ggtgaaagct cagaaacgat gtttgctcga catgcagatt
     6121 ttgagtagta gattttaaag ataaaaaacc attctctaaa tgctaattat tcagcaaata
     6181 tgagaatggt ttttttattt gattagacat aacttaataa ttcagtagtt ttgagcgtaa
     6241 gcgtgtcata gaatggtacc taaacacaaa tagcccgatt tgatacactc aaatcaggtt
     6301 atttgtggct tatattattt acagatttct tgtatctgct gattccatgg caaataagcg
     6361 cgtagcgctt caggtgtcat cgaagacaga ttgggaatct ctgtaaaaag atgatttaaa
     6421 tatttgcgga tgtttaaccc attcgcaaca gctgtctgga taatactcaa tataattcca
     6481 tttgatctag cgccttttaa gcttttagaa aatagccagt tttttcgacc gatcactaac
     6541 tctttgatgg cacgctctgc tcggttattt gaaagttcca agcgtccatc aagcaacaca
     6601 atctgaagtc cgtctttatg attgactgcg tattgaaccg cctttccaag ctgcgatttt
     6661 ggcaaataag caaaactctc acaccaatga aaaaattcag tcatcacagg ctgcaacgtt
     6721 tccttcctcg ctacaaacct ttcttctgcg ctaagcttat taaaggtccg ttcgattcga
     6781 aacatcttgt cacagaagtc taatcctatt ttggcatgac tttttgggga cggttgttta
     6841 cccaatgctt caaaaaactt tctgcgaaca tgcgcccaac aataaacatt gtttaccggc
     6901 agctttccat aggccgcgta accatcagta tgcaagaaac cgctaaatga ttctaaaaaa
     6961 ctttttggct catcaacgga acgcgaattt gcgtgctcgt agagcacaac ttgtttttct
     7021 tcgcttttac ctgaagcata taaccagtaa aaggtctttt ctttatgagt attcaatacc
     7081 cgatagcttg tctcatccgc gtgtaagacc tcctgattgc ttaattcttg atgaaggcaa
     7141 ttgtagattg gctctaaaac catttcactt accttgatat gccaattagt gattttttta
     7201 cgatcaagag ggaaaccaag tgcatgccat tctttttctt gtcgatatgc cggcaccttt
     7261 ttaacaaatt tttcccaaat actctgtgtc acaattgaag aggaacctag actattattg
     7321 atcaacggtt taggaacagg cgcttttttg atttttgttt ctcctaatgc ttcacattct
     7381 ctgcaatgat aggcatgttg gtagatgact tgtttttcta tatgggcagg aacatacaat
     7441 ggctcttctc taagccttgt cgcaccgata tcatgtaaag tatgaccaca gtcttcacac
     7501 aaacaatcgg caccttttaa ttcataatga ataggaactt caggcagacc ttctaatgaa
     7561 agtgctttga gtcccttgcg ttttctgcgt ttaaaagaag ataccgtaat gggttcctgg
     7621 ctttcttgtc cagtgtgctc tggttcatta aaagttgagt cgtctaataa atcgatttgc
     7681 ccatctacgg gtggtgtttc tttttttcgt ccaaacaagc gtcgattcat ttcagcaatt
     7741 tgttctctaa gaagagtatt ttcctgttgc tggatttcaa gcaactgtgt taattgcttg
     7801 atttgtacaa gaagttcaga ttccattttt gtcattttta caccaccatt ctagcgattt
     7861 tcttacatct attataccat tttatgacaa cgaaaaacag acaaaatcct tgatttaaca
     7921 aggttttcgt ctaatatatc gcaccttttt tcgtttcttt tagcgtacat ttttcttcga
     7981 ttgaaaggcc agtcagtaag acttcttttc tggaacgcgg ccattgaagg tacccacttt
     8041 caaatctttt gtacattaat acaaatccat tttgatccta gtaaagagcc ttgaagcgat
     8101 ctttcttcgt accgcaaaat aagaaaagcg cttgatcgta aacatcaagg tcatattctt
     8161 ctataacgaa agctgctagt ccatcaattc cttttcgaag atctgttttt ccacaaacga
     8221 taaaaatatg tttcaatgca gttgcatcaa tgagcattgg cattcaactc ttttaacaag
     8281 gtatacaaaa gatatttatc cattccatta taaataaata tttccttatc ttccgatttg
     8341 attctgcaag ctagttttgg cttggtgtca atttgcgatt tttgagccgc acttgaaaat
     8401 tgaactggta caatatcttt tttattcatt cgtttttctc cttctatatt tgatagttta
     8461 attatagaag aaagataatc ggcgtacgag atactatcct atgacacgct tacatttaat
     8521 ttaatatttt gcctcaaatt agccacactt tttatagaac taagaggaaa aatgaagttt
     8581 tcttcctatt aatatagttg tttgaaatct taaatatagc gttttacgat gtaatgggaa
     8641 gtatggtgaa taaatcaact atgttcgtac gaagcattta ctcaactaat ttaatcgaat
     8701 ctttcaataa acaaatcaag aaatacagcc accgcaagga acagttccaa aatgaagagt
     8761 cgatggaacg tttcttagtc tcgtcttttg atacttacaa ccaaaaattc ctaggtcgca
     8821 gtcataaagg tttccaacag gccgaaggcg agctcgaaca aatgctgagt caaccgatgg
     8881 agaattagaa acaatctaca gtagggaaga accattgaca caaaattatt gacgctccca
     8941 actaaaaatg tcaaaataat gatgaaatca tttagttctt ataaattaga aaagagaaaa
     9001 taaatattcc atatctactt tattggatcc gtaacatgac taaattctga atacttttta
     9061 aagcttatca tttggtaaaa ctgaatataa gttaattata tagactgaaa attttgaatt
     9121 ttcagaatag aataacttaa aagtactgac aaaatttttt aaaggggcaa tattaatgtt
     9181 ctgagataat caaatcctac cgaaaatatc aaaaatccaa tgtgacacaa tcagattgtg
     9241 tctcattcga gggaatcaca caatcccacg tggctctagg cagaattgaa tttttgcttg
     9301 gggctttttc cttatattga ttttgaatga gacttattat atgttataat gcaattaagt
     9361 atcattggag agggcgaaga aaatgaagac ggtagaacct attcgagaca aaaaaaagat
     9421 cgatgcgatg aaggcaatct tggcttcggg aaaatatggc caacgaaatt tggttctttt
     9481 ttcgattggg atcaacaccg cctatcgcat ttctgatttg cgtcaattga agctggccga
     9541 cgtgctggaa atttcacgcg gtcgcgtggt ggtcaaagat cgattagcga tgaaggaaca
     9601 aaaaacagct aaacataatt cggtctatat ttccaacaaa ttgcgaaaaa cgattttgga
     9661 gtacgttcaa caggaatttc ctgatcaact ccttcagcaa gattttgacg gttatttgtt
     9721 ccctagtcga aaagggacga atgaaccgct caaccgccaa agtctgtggc gaatcattca
     9781 tgatgctggc acggcaatcg gattgaaaga cattggccca cattccatgc gaaaaacgtt
     9841 cggttacttt ctctataaac aggggaccaa aacagaaatc attcagtcct tgttgaacca
     9901 ttcttctcag cgcgaaacat tgcggtatat tggtattaca caagaagaca aagatacagc
     9961 tgtaaaaagc ttagatttat aatataaaag ttaagtttaa acatggatag ttttaaatta
    10021 gtagattgag tgttgggagg aaagaatgag tacattatat attataggaa acggttttga
    10081 tttaagtcat ggtttaaaaa ctagatacac tgatttttca aaatgggttg aaaaaagtca
    10141 tagtgaaata ttcgataaaa tagacacaat gataaatgaa aataatgagg tgagaagtac
    10201 agctgatgat aagataggtt ggtttaattt tgaaagtagt ttgcatgagt taattcaaag
    10261 tccagtcttt gaagattatg tagaagagtc taaggattat ctcataggtt atggacatga
    10321 agattggtca gattctgccc atcatgattt tcaaaatatg gtttatgatt atgttgtttc
    10381 tctagaagaa ttacaacaac ttttcaagga atggatacgc aaagaaatac agccgacaat
    10441 agaacataaa attccaaaat accaattttc gtccgatgta ctattcttta cattcaatta
    10501 tacagatgtt ttggaaattc tatacaagca accgaaagaa aacatatatc atattcatgg
    10561 cagttatgac gatataataa tcggccactc cgagaatttt gacaacttta taggaga
//
LOCUS       DACGLE010000087        10534 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0534
            SAMEA3304449-rid9560453.denovo.092, whole genome shotgun sequence.
ACCESSION   DACGLE010000087 DACGLE010000000
VERSION     DACGLE010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304449
            Sequence Read Archive: ERR985064
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10534)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10534)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702225.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702225.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 20:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,045
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 27 of 67
            Pseudo Genes (incomplete)         :: 25 of 67
            Pseudo Genes (internal stop)      :: 19 of 67
            Pseudo Genes (multiple problems)  :: 4 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10534
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304449-rid9560453.denovo.092"
                     /strain="VREN0534"
                     /db_xref="taxon:1352"
     gene            complement(1..657)
                     /locus_tag="IXT20_002401"
                     /pseudo
     CDS             complement(1..657)
                     /locus_tag="IXT20_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333400.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
     gene            complement(662..1429)
                     /locus_tag="IXT20_002402"
     CDS             complement(662..1429)
                     /locus_tag="IXT20_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAQ4860825.1"
                     /translation="MNKKRRLEKILDMLKIDGTITIKEIIDELDISDMTARRDLDALE
                     ADGLLTRTHGGAQLLSSKKPLEKTHIEKKSLNTKEKIDIAKKACSLIKDGDTIFIGPG
                     TTLEQLALELKGRKGYKIRVITNSLPVFLILNDSETIDLLLLGGEYREITGAFVGSMA
                     STNLKAIRFAKAFVSANAVTHNSIATYSDKEGVIQQLALNNAVEKFLLVDSTKFDRYD
                     FFNFYNLDQLDTIITDNQISPQHLEEFSQYTTILKAD"
     gene            1917..2342
                     /gene="lacA"
                     /locus_tag="IXT20_002403"
     CDS             1917..2342
                     /gene="lacA"
                     /locus_tag="IXT20_002403"
                     /EC_number="5.3.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005237726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacA"
                     /protein_id="HAQ4860826.1"
                     /translation="MAIVVGADLKGTRLKDVVKNFLVEEGFEVIDVTKDGQDFVDVTL
                     AVASEVNKDEQNLGIVIDAYGAGPFMVATKIKGMVAAEVSDERSAYMTRGHNNSRMIT
                     VGAEIVGDELAKNIAKGFVNGKYDGGRHQIRVDMLNKMC"
     gene            2359..2874
                     /gene="lacB"
                     /locus_tag="IXT20_002404"
     CDS             2359..2874
                     /gene="lacB"
                     /locus_tag="IXT20_002404"
                     /EC_number="5.3.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacB"
                     /protein_id="HAQ4860827.1"
                     /translation="MRIAIGCDHIVTDEKMAVSEFLKSKGHEVLDFGTYDHARTHYPI
                     YGKKVGEAVVSGQADLGVCICGTGVGINNAVNKVPGVRSALVRDMTSAIYAKEELNAN
                     VIGFGGKITGGLLMNDIIEAFIEAEYKPTEENKKLIAKIEHVETHNAHQADEDFFTEF
                     LERWDRGDYHD"
     gene            2885..3817
                     /gene="lacC"
                     /locus_tag="IXT20_002405"
     CDS             2885..3817
                     /gene="lacC"
                     /locus_tag="IXT20_002405"
                     /EC_number="2.7.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-6-phosphate kinase"
                     /protein_id="HAQ4860828.1"
                     /translation="MILTVTLNPSVDISYPLETLKIDTVNRVKDVSKTAGGKGLNVTR
                     VLYESGDKVTATGFLGGKIGEFIESELEQSPVSPAFYKISGNTRNCIAILHEGNQTEI
                     LEQGPTVSHEEAEGFLDHYSNLIKQSEVVTISGSLPSGLPNDYYEKLIQLASDEGVAV
                     VLDCSGAPLETVLKSSAKPTAIKPNNEELSQLLGKEVTKDIEELKDVLKESLFSGIEW
                     IVVSLGRNGAFAKHGDVFYKVDIPDIPVVNPVGSGDSTVAGIASALNSKKSDADLLKH
                     AMTLGMLNAQETMTGHVNMTNYETLNSQIGVKEV"
     gene            3820..4800
                     /gene="lacD"
                     /locus_tag="IXT20_002406"
     CDS             3820..4800
                     /gene="lacD"
                     /locus_tag="IXT20_002406"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HAQ4860829.1"
                     /translation="MVLTEQKRKSLEKISDKNGVISALAFDQRGALKRLMAQYQDTEP
                     TVAQMEELKVLVADELTKYASSMLLDPEYGLPATKALDKEAGLLLAYEKTGYDTSSTK
                     RLPDCLDVWSAKRIKEQGADAVKFLLYYDVDSSDELNQQKQAYIERVGSECVAEDIPF
                     FLEILAYDEEISDAGSVEYAKVKPRKVIEAMKVFSDPRFNIDVLKVEVPVNVKYVEGF
                     ADGEVVYSKAEAADFFKAQEEATNLPYIYLSAGVSAKLFQETLQFAHDSGAKFNGVLC
                     GRATWAGSVEPYIKEGEKAAREWLRTTGFENIDELNKVLVKTASPWTDKV"
     gene            4828..5145
                     /locus_tag="IXT20_002407"
     CDS             4828..5145
                     /locus_tag="IXT20_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011669100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HAQ4860830.1"
                     /translation="MNREEMTLLGFEIVAYAGDARSKLLEALKAAENGDFAKADSLVV
                     EAGSCIAEAHNSQTAMLAREASGEELPYSVTMMHGQDHLMTTVLLKDVIHHLIELYKR
                     GAK"
     gene            5145..6851
                     /locus_tag="IXT20_002408"
     CDS             5145..6851
                     /locus_tag="IXT20_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ4860831.1"
                     /translation="MHKLIELIEKGKPFFEKISRNIYLRAIRDGFIAGMPVILFSSIF
                     ILIAYVPNAWGFHWSKDIETFLMTPYSYSMGILAFFVGGTTAKALTDSMNRDLPATNQ
                     INFLSTMLASMVGFLLMAAEPAKEGGFLTAFTGTKGLLTAFIAAFVTVNVYKVCVKNN
                     VTIRMPEEVPPNISQVFKDLIPFTVAVVLLYGFELIVKGTLGVTVAESIGTVLAPLFS
                     AADGYLGITLIFGAYAFFWFVGIHGPSIVEPAIAAITYANIDANLHLIQAGQHADKVI
                     TSGTQMFIVTMGGTGATLIVPFLFMWICKSERNRAIGRASVVPTFFGVNEPILFGAPI
                     VLNPIFFVPFIFAPIVNVWIFKFFVDTLNMNSFSANLPWVTPGPLGIVLGTNFQVLSF
                     ILAGLLVVVDTIIYYPFVKVYDEQILEEERSGKTNDALKEKVAANFNTAKADAVLGKA
                     GVAKEDVAANNNITKETNVLVLCAGGGTSGLLANALNKAAAEYNVPVKAAAGSYGAHR
                     EMLPEFDLVILAPQVASNFDDMKAETDKLGIKLAKTEGAQYIKLTRDGQGALAFVQQQ
                     FD"
     gene            6993..8399
                     /gene="lacG"
                     /locus_tag="IXT20_002409"
     CDS             6993..8399
                     /gene="lacG"
                     /locus_tag="IXT20_002409"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HAQ4860832.1"
                     /translation="MTKTLPKDFIFGGATAAYQAEGATHTDGKGPVAWDKYLEDNYWY
                     TAEPASDFYHKYPVDLELAEKYGVNGIRISIAWSRIFPTGYGEVNEKGVEFYHNLFAE
                     CHKRHVEPFVTLHHFDTPEVLHSDGDFLNRENIEHFVDYAAFCFEEFPEVNYWTTFNE
                     IGPIGDGQYLVGKFPPGIQYDLAKVFQSHHNMMVSHARAVKLYKDKGYKGEIGVVHAL
                     PTKYPYDPENPADVRAAELEDIIHNKFILDATYLGHYSDKTMEGVNHILAENGGELDL
                     RDEDFQALEAAKDLNDFLGINYYMSDWMQAFDGETEIIHNGKGEKGSSKYQIKGVGRR
                     IAPDYVPRTDWDWIIYPEGLYDQIMRVKNDYPNYKKIYITENGLGYKDEFVDNTVYDD
                     GRIDYVKKHLEVLSDAIADGANVKGYFIWSLMDVFSWSNGYEKRYGLFYVDFDTQERY
                     PKKSAHWYKKVAETQVIE"
     gene            8422..8694
                     /locus_tag="IXT20_002410"
     CDS             8422..8694
                     /locus_tag="IXT20_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3884 family protein"
                     /protein_id="HAQ4860833.1"
                     /translation="MLSDTLEKVQHLFRPGFEKVYIVSFEDCPMIPELEATPLLKCGK
                     WYVSTGKEWICHSDLELSAFEWEFLQSLDVEIRETIHFEVNYLPFQ"
     gene            8715..9614
                     /locus_tag="IXT20_002411"
     CDS             8715..9614
                     /locus_tag="IXT20_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002889672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HAQ4860834.1"
                     /translation="MTIELKNEYLTVQFKTLGGQLTSIKDKDGIEYLWQADPNYWNGQ
                     APILFPICGSLRNDWAIYRPQERPFFTGLIRRHGFVRKEEFILEEVNDNSVTFSIKPN
                     AEMLDNYLYQFELRVVYTLNGKSIRTEFQVTNLEMEKTMPYFIGAHPAFNSPLVEGEN
                     YEDYYIEFSEVESCSIPKSFPETGLLDLQDRTPFLENQKILDLDYSLFSHDAITLDQL
                     KSRSVTLRSRKSGKGLRVDFDDFPNLILWSTTNKSPFIALEPWSGLSTSLEEGNFLED
                     KRQVTKISPQETSRKIYDITILN"
     gene            complement(10086..>10534)
                     /locus_tag="IXT20_002412"
     CDS             complement(10086..>10534)
                     /locus_tag="IXT20_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAQ4860835.1"
                     /translation="FNREILGYSCGEHKDAVLVKKAFSRIKQPLTEVEIFHTDRGKEF
                     DNQAIDELLTTFDINQSLSHKGCPFDNAVAESTYKSLKVEFVYQYTFETLQQLDLELF
                     DYVNWWNHLRLHGTLGYETPVGYRNQRLAQRILDNELGCANASEAV"
BASE COUNT         3303 a         1798 c         2108 g         3325 t
ORIGIN      
        1 gtttatgacg acttttggaa gtaaaaatcc atccattatc tttataatca ggattgaatc
       61 ttatatgttg gtgatttaac tcaatttcct ctttaagaat ttctatagcc cttttagata
      121 attggacatt ttggtaactt tgtaagtttt ttacattacc ttcatagcta ttttctgcag
      181 ctcctccgtg agtggactga ctaatgatga tattttgctt ttcaaaatat tgataggcac
      241 caatattttg gagttttcga ctctgtaagt caacagggcc attagttaga atacctattg
      301 ggatgcggtt tttatgtaga gccagtagta gttgatgtat ctcaggaatc atcgtaatct
      361 gatcgagttc atattcatag tcagcttgga agtctagagc ttctgtccta gaaatactat
      421 cgatgccaaa ttcttccata gtcttctgac agcgaaaaat tctaagctgc tcaatactga
      481 tgagtttttc gtgtatttag gaaaagcaac atcagaccaa catctgaaac gtttgtaaat
      541 gttatctatt tgttggatat caatcgttgg aaaacatttc tcaacgctac ttttaaaggg
      601 gagttgatgg tcataaagag tgtcatcaag gtcaaaaaca atatttttat aagtcataat
      661 tctagtccgc ttttaaaata gtagtgtact ggctaaattc ctctaagtgt tgagggctaa
      721 tctggttatc tgtaatgatg gtatcgagtt gatctagatt atagaagtta aagaaatcgt
      781 atcgatcgaa tttagtactg tctactaata agaatttttc tacagcattg tttagggcaa
      841 gttgttgaat cacaccttcc ttgtcactat atgtagcaat agaattatgg gtaacagcat
      901 ttgcactaac aaaagctttg gcaaatctaa ttgcttttaa atttgtcgaa gccattgaac
      961 ctacaaaagc tccagttatt tctctatatt caccgccaag aagcaataaa tcaatggttt
     1021 cgctatcatt tagaatcaag aacacaggga gactatttgt aatgacacga attttataac
     1081 ctttacgacc tttcaattct aatgccagtt gttcaagtgt agttccgggt ccaataaaaa
     1141 tagtatcgcc atctttgatt aaagagcagg cttttttagc aatgtcaatt ttttcttttg
     1201 tatttagact tttcttctcg atatgtgtct tttcaagtgg ctttttagag gaaagtaatt
     1261 gtgcaccacc atgagtacgt gttaaaagtc catcagcttc tagagcatca aggtctctac
     1321 gggctgtcat atcggaaata tctagttcat ctattatttc ttttatggtt atggtcccat
     1381 caatctttaa catatctaaa attttttcta atcgtcgttt tttgttcata tgaagaccct
     1441 tctttcataa agtaattttt ttccaaagat aattctcttt taattgtatc ataaaagaca
     1501 atattttcaa ggtaaaacaa acaatttcaa acaaaaacaa atgttagatg atgaaataag
     1561 aacagtggat tgacgtatat tagcttaggt cagattttgt ataagacgaa aataaagtag
     1621 gacctcttaa tcagtaagtt atagaaagta aaagactttt gtaatacttg aatagatatt
     1681 tcacgtccat tttgtgatgg attaaatgaa caaaaatgaa caataattta acggtgttat
     1741 ctatttttta aaaaaacaaa taaaaaaaca aaaaataaac aaaaaagtgt tgctttttgt
     1801 ttgaatgctt gatattatat aaacaaagaa atgatgaaaa cgttttttcg aacattttgc
     1861 aaaatgtttt ctacttctac gtagcatttc tttctaaaat ttaggaggtg gtcaaaatgg
     1921 ctattgttgt tggtgcagat ctcaaaggta cgagattaaa agatgttgtg aaaaattttc
     1981 ttgtagaaga aggttttgag gtaattgatg tgactaagga cggacaagat tttgttgatg
     2041 ttaccttggc agttgcctct gaagtaaata aagatgagca aaacttaggt attgtaattg
     2101 atgcttatgg agctggtcca ttcatggtag caactaaaat taaaggtatg gttgcagcag
     2161 aagtttcaga tgaacgctca gcttatatga cacgtggaca taacaattca cgaatgatta
     2221 cggttggcgc tgaaatcgtc ggtgatgagc ttgctaaaaa catcgctaaa ggtttcgtca
     2281 atggtaaata tgatggcgga cgtcatcaaa tccgagtaga tatgcttaac aaaatgtgtt
     2341 aaggaaagga gttaaggaat gagaatcgca attggatgtg accacattgt cacagatgaa
     2401 aaaatggcag tatcagaatt cttgaaatca aaaggacatg aggtccttga tttcgggaca
     2461 tatgaccacg cacgtactca ctaccctatc tatggtaaaa aagtcggtga agcagtagta
     2521 agtggtcaag cagacttggg agtatgtatc tgtggtacag gtgtcggcat taacaatgca
     2581 gtcaataaag ttccaggtgt tcgctcagcg ttggttcgtg atatgacatc agctatatat
     2641 gcaaaagaag agttaaatgc gaatgttatc ggtttcggtg gaaagattac tggtggtctt
     2701 cttatgaatg acatcattga agctttcatt gaagctgagt acaaaccaac agaagaaaat
     2761 aaaaaattga ttgcaaaaat cgaacacgtt gaaacacata atgcacatca agcagatgaa
     2821 gatttcttta cagaattcct tgaaagatgg gaccgtggtg actaccacga ttaaggtata
     2881 acaaatgatt ctgacagtca cactaaatcc ttcagtagac atctcttatc ctctagaaac
     2941 attgaaaatt gatacagtga atagggtgaa agatgttagt aagacagctg gtggaaaagg
     3001 cttaaatgtt actagagtct tgtatgaatc tggtgataaa gttactgcta caggtttctt
     3061 aggtggtaaa attggtgaat ttattgaaag cgaattagaa caatctcctg ttagtccagc
     3121 tttttataaa atctcaggaa acactagaaa ctgtattgct atcttacacg agggtaacca
     3181 aacagaaatt ttggaacaag gaccaacagt ttctcatgaa gaagcagaag gttttcttga
     3241 ccactatagc aatcttatta aacaatcaga agttgtgaca atttcaggaa gtttaccttc
     3301 aggacttccc aacgattatt atgaaaaact tatccaactt gcttcggatg aaggagtagc
     3361 agtcgttttg gattgttcag gtgcacctct tgaaacagtt ctaaaatcgt cggctaaacc
     3421 tactgccatc aaacctaata atgaggaact ttcacagttg cttggaaaag aagtaacaaa
     3481 agatattgaa gaactcaaag acgttcttaa agagtcactt tttagtggta tcgaatggat
     3541 agtggtctca ttaggtcgaa acggtgcctt tgcaaaacat ggtgatgttt tctataaggt
     3601 agatattcct gatatcccag ttgttaatcc agtcggatca ggggactcaa cggtagctgg
     3661 tattgcatca gctttaaata gtaaaaaaag tgacgctgac ttattaaaac atgcgatgac
     3721 attgggtatg ttaaatgctc aagaaacaat gacagggcat gtgaatatga ctaattacga
     3781 aacactaaac tcacaaattg gagtaaaaga ggtataaaaa tggtacttac agaacagaaa
     3841 cgcaaatctt tggaaaaaat ttcagataaa aacggtgtta tctcagcttt ggcatttgac
     3901 caacgtggtg ctttgaaacg tttgatggca cagtatcaag atacagaacc aactgtagct
     3961 caaatggaag aactaaaggt tttggttgct gatgaactta caaaatacgc ttcttcaatg
     4021 ttgcttgacc ctgaatatgg acttccagca acaaaagcat tggataaaga agcaggtctt
     4081 cttcttgcct atgaaaaaac aggttatgat acatcaagta caaaacgttt gcctgactgc
     4141 ttggatgttt ggtctgcaaa acgtatcaaa gagcaaggcg ctgatgccgt taagttcttg
     4201 ctttactatg atgtagacag ctcagatgaa cttaaccaac aaaaacaagc ttatattgaa
     4261 cgtgttggtt ctgaatgtgt cgctgaagat attcctttct tcttggaaat ccttgcttat
     4321 gatgaagaaa tctcagatgc aggttctgtt gaatatgcaa aagtgaaacc tcgcaaagtc
     4381 attgaagcga tgaaagtttt ctctgatcca cgctttaaca ttgatgttct taaagtagaa
     4441 gtaccagtta acgttaaata tgtcgaaggt tttgctgatg gtgaagtggt ttacagtaaa
     4501 gctgaagctg ctgacttctt taaagcacaa gaagaagcga ctaaccttcc atacatctac
     4561 ctaagtgcag gtgtatctgc taaattgttc caagaaacat tgcaatttgc tcacgactca
     4621 ggtgccaagt ttaatggtgt gctttgtgga cgtgctactt gggcaggatc tgtagaacct
     4681 tacatcaaag aaggtgaaaa agcagcacgt gaatggttgc gtactactgg attcgaaaat
     4741 attgatgaac ttaacaaagt tcttgttaaa acagctagtc catggactga taaagtatag
     4801 taaaaacata aaacggagga tattgttatg aacagagaag agatgactct cttagggttt
     4861 gaaattgttg cttatgctgg agatgctcgc tctaaacttt tagaagcgct taaagcggct
     4921 gaaaatggtg atttcgctaa ggcagatagt cttgtagtag aagcaggaag ctgtattgca
     4981 gaggctcaca attctcagac agctatgttg gctcgagaag cttctgggga ggaacttcca
     5041 tacagcgtta ccatgatgca tggtcaggac cacttaatga ctactgtctt attaaaagat
     5101 gtgattcatc acctcatcga actttataaa agaggagcaa aataatgcat aaactcattg
     5161 aacttattga gaaagggaaa ccgttctttg agaaaatctc tcgaaacatc tatcttcgtg
     5221 ctattcgtga tggatttatc gctggtatgc cagtcatcct tttctcatct atctttatcc
     5281 ttattgccta tgtcccaaat gcttggggat tccactggtc aaaagacatt gagaccttct
     5341 tgatgactcc ttatagctat tcgatgggta tcctagcatt ctttgttggt ggtactaccg
     5401 ctaaagcttt gacggactca atgaaccgtg acctacctgc gaccaatcag attaacttct
     5461 tatctacgat gctagcgtct atggtagggt tccttttgat ggcagctgag cctgcaaaag
     5521 aaggaggctt cttgacagcc ttcacgggaa caaaaggtct tttgacagca tttattgcag
     5581 cctttgtgac agttaatgtt tacaaggtct gtgtgaaaaa taatgttact attcgtatgc
     5641 ctgaagaggt tccaccaaat atctctcaag tatttaaaga cttgattcca ttcactgtag
     5701 cggttgttct cctttatgga tttgaactca ttgttaaggg aactcttggt gtaaccgttg
     5761 cagaatcaat cggtaccgtt cttgctcctc tcttctcagc tgcagatggt taccttggaa
     5821 ttacacttat ctttggtgct tatgccttct tctggtttgt tggtatccac ggtccttcaa
     5881 ttgtcgaacc agcaatcgct gctatcactt atgccaatat cgatgccaac ttgcatctta
     5941 tccaagctgg acaacatgca gataaagtta tcacttctgg tactcaaatg tttattgtta
     6001 ccatgggtgg aacaggagct acattgattg ttccattctt gttcatgtgg atttgtaaat
     6061 cagaacgtaa ccgtgccatc ggacgtgcct cagttgttcc aacattcttt ggggttaatg
     6121 agccaatctt atttggtgca ccaatcgtat tgaatccgat tttctttgta ccgttcattt
     6181 tcgctccgat tgtcaacgtt tggatcttta aattctttgt tgacacattg aacatgaact
     6241 cattctctgc caaccttcca tgggttactc ctggtccgtt gggaattgta ctcggtacta
     6301 acttccaagt tctatcattt atcttagccg gactcttggt agttgttgat actatcattt
     6361 attacccatt tgttaaggta tacgatgaac aaattcttga agaagaacgt tctggtaaaa
     6421 caaacgatgc tcttaaagaa aaagtagctg caaacttcaa tactgctaaa gcagatgccg
     6481 ttcttggaaa agcaggcgtt gctaaagaag atgtagcagc aaacaataat atcacaaaag
     6541 aaactaatgt ccttgttctt tgtgcaggtg gaggtacaag tggcttgctt gccaatgcct
     6601 tgaacaaagc agcagcagaa tacaatgttc cagtcaaagc ggcagcgggt agttacggtg
     6661 ctcaccgtga aatgttgcca gagtttgact tggttatctt ggctccacag gtcgcttcaa
     6721 acttcgatga tatgaaggct gaaacggata aattgggcat taaacttgca aaaactgaag
     6781 gagctcaata tatcaaattg acacgtgatg gacaaggtgc tcttgcattt gttcaacaac
     6841 agtttgatta ataaatttgt aaaagttatt tgagacgaga ttattctttc agttccttat
     6901 aatattgtta accatatcat tgcatttcat ttccttggtt tccggaactc agaatatcgt
     6961 ctcaaatgct ttttttgaaa ggatttatag ttatgactaa aacacttcct aaagatttta
     7021 tttttggtgg tgctacagct gcttatcaag cagaaggtgc gactcacacg gatggtaaag
     7081 gcccagttgc atgggataag tatcttgaag acaattattg gtacacagca gagcctgcaa
     7141 gtgattttta ccataaatat cctgttgatt tagaattagc tgaaaaatat ggcgttaatg
     7201 gtattcgtat ttcaattgcc tggtctcgta ttttcccaac tggctatggc gaagtaaatg
     7261 aaaaaggtgt tgaattctac cataatcttt ttgccgagtg ccacaaacgt catgttgagc
     7321 cattcgtgac tttgcaccac tttgacacac cagaagttct tcactcagat ggagatttct
     7381 taaaccgcga aaatatagaa cactttgtag attatgccgc tttctgtttt gaagagttcc
     7441 cagaagtaaa ctactggaca actttcaatg aaattggccc aattggagat ggtcaatact
     7501 tggttggtaa attccctcca ggcattcaat atgatcttgc caaagtcttc caatcacacc
     7561 acaatatgat ggtttctcat gcacgtgctg tgaaattgta taaagataaa ggttataaag
     7621 gcgaaattgg tgtagtacat gcgttgccaa ccaaataccc ttatgatcca gaaaatccag
     7681 cagatgttcg tgccgctgag ttagaagata tcatccataa caaatttatc ttggatgcaa
     7741 cctaccttgg acactattca gataaaacga tggaaggggt caaccacatc ctagctgaaa
     7801 atggtggaga acttgatctt cgtgatgaag acttccaagc acttgaagca gctaaagatt
     7861 tgaacgattt ccttggtatc aactactaca tgagtgattg gatgcaagcc tttgatggtg
     7921 agactgaaat cattcacaat ggtaagggtg aaaaaggaag ttctaagtac cagattaaag
     7981 gtgttggtcg ccgaatcgca ccagattatg tgcctagaac ggattgggac tggattattt
     8041 atcctgaagg cttgtatgac caaatcatgc gagtgaaaaa tgattatcca aactacaaga
     8101 agatttacat cactgaaaac ggtcttggat acaaagatga gtttgtagat aataccgttt
     8161 atgatgatgg tcgtattgat tacgtgaaga aacacttgga agttttatca gacgcaattg
     8221 cggatggtgc aaatgttaaa ggttacttca tctggtcact tatggatgtc ttctcatggt
     8281 caaacggata cgaaaaacgt tatggactat tctatgtaga ctttgacacg caagaacgct
     8341 atcctaagaa atcagctcat tggtataaga aagtagcaga aactcaagtg atagagtaat
     8401 attacttgaa aatgtaaaaa attgttatcc gatactttag aaaaagtaca acacttattt
     8461 agaccaggct ttgaaaaggt atatattgtt agtttcgaag attgtcctat gattcctgag
     8521 cttgaagcta ccccgttgct aaaatgtggt aaatggtatg tttcaactgg aaaagagtgg
     8581 atttgccatt ctgatttaga gttatcagca tttgaatggg aatttttaca atcactcgat
     8641 gtagaaatac gtgagactat tcacttcgag gtaaattatt tgcctttcca ataataaaaa
     8701 gaaaggaatt agttgtgact atcgaattaa aaaacgagta tcttactgtt cagtttaaaa
     8761 cacttggtgg tcaactgact tcgattaaag acaaggatgg gatagagtat ctctggcaag
     8821 ctgatccaaa ttattggaat ggacaggcgc ctattctatt tcctatctgt ggtagtttac
     8881 gaaatgattg ggctatttat agacctcaag aaagaccttt ttttacaggt cttattcgta
     8941 gacatggttt tgttcgaaaa gaagaattta ttctggaaga agtcaatgat aatagcgtga
     9001 cctttagtat taaaccaaat gctgagatgc ttgataatta cctatatcag tttgaactga
     9061 gagttgttta taccttgaat ggtaaatcaa ttagaaccga atttcaggta acgaacttgg
     9121 aaatggaaaa aacaatgcct tactttattg gagcccatcc agcattcaat agtcctttag
     9181 tagaaggtga aaactatgag gattattata ttgaatttag tgaggttgag tcttgctcga
     9241 taccaaagag ttttcctgag acaggattat tagatttgca agatcggaca ccttttttgg
     9301 aaaatcaaaa aattctcgat ttggactact cacttttttc ccatgatgcc attacacttg
     9361 accagctcaa gtctcgtagc gtaacgcttc ggtcaaggaa gtctggtaaa ggtcttcgag
     9421 tagattttga tgattttccg aacttgattc tgtggtctac aactaataaa agtccattca
     9481 ttgctttgga accttggagt ggtctctcca cttcacttga agaaggtaat ttcttagaag
     9541 ataaaagaca ggtaacgaaa atttcacctc aagaaacttc tagaaaaatc tatgatatta
     9601 ctatccttaa ttaaaatttg ttattatttg agtcttcaat aagatttgaa tactcttacc
     9661 ttgtgatgat agattggtct tctccgtgcc agtcttacat cgcaaggttt ttaagttttt
     9721 tgtgggtcta tgtcaactgt agtgggtgac gaaaagctaa tatctaaaga cagtctggta
     9781 gtaaaaaata agattattta ctacttcttg atttaaaata atagtggagc cattaatttg
     9841 tggcaaattt aatttaatat tttgcctcaa attagccaca ctttttatag aactaagagg
     9901 aaaaatgaaa ttttcttcct attaatatag ttgtttgaaa tcttaaatat agcgttttgg
     9961 aatggcaaca gtttttttga caaattttat aaggtgcaga acttctttcc gtatgctatt
    10021 ccgattgtcc ttgacaatga gcctcctcgg atgtgacgat cttcggcagg agctaacagt
    10081 taaagttaga ctgcctcgct agcgttagca catccgagct cattatcaag gattcgctgc
    10141 gccaatctct ggttacggta accaaccggt gtctcgtagc caagtgtacc gtgcaaccga
    10201 aggtggttcc accaattgac atagtcaaat aactccaaat ccaattgttg taaggtttca
    10261 aatgtgtatt gatagacaaa ttctactttc agcgacttat aagttgattc agctacggca
    10321 ttatcaaaag gacagccttt atgactcaat gattgattga tgtcaaaagt tgttaataat
    10381 tcatcaatag cttggttatc aaactctttt ccacgatcag tatgaaaaat ctcaacctct
    10441 gtcagaggtt gtttgatacg gctaaatgct ttttttacta gaacggcatc tttatgttct
    10501 ccacaagaat agccgagaat ttctcgattg aaca
//
LOCUS       DACGLE010000088        10468 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0534
            SAMEA3304449-rid9560453.denovo.093, whole genome shotgun sequence.
ACCESSION   DACGLE010000088 DACGLE010000000
VERSION     DACGLE010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304449
            Sequence Read Archive: ERR985064
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10468)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10468)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702225.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702225.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 20:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,045
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 27 of 67
            Pseudo Genes (incomplete)         :: 25 of 67
            Pseudo Genes (internal stop)      :: 19 of 67
            Pseudo Genes (multiple problems)  :: 4 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10468
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304449-rid9560453.denovo.093"
                     /strain="VREN0534"
                     /db_xref="taxon:1352"
     gene            103..1146
                     /locus_tag="IXT20_002413"
     CDS             103..1146
                     /locus_tag="IXT20_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyhydrolase"
                     /protein_id="HAQ4860836.1"
                     /translation="MKKFLVGILFTMGLVLLSPSVSAESSISMYRMYNPNSGEHLYTR
                     SVGERDNLKRYGWHYEGVAWQAPTSGTPVYRLYNRYNGEHFYTVDAGERDKISHTGWT
                     YEGVAFYSYNGANRVPVTRLYNKYVNWHHYTLDQNEKNVISKQGWKIEGTGWYAMPNS
                     VPNPTPAPSTEKVLNVPVVWQGNTMLCEGASLLEGLHYKGVATNQSLTSFVNYMPKAN
                     DNNPYHGYSGEWRHNVNGTYQGMMADPVVKWAKNVGGNAVNITGSGTNGIKNEIRNGN
                     PVVTWITYNYATPEFKQMPWGRAVWNGHVVLVDGFRNGAYHVVDPVFGAKWINASTYE
                     RAFNVTGMAVAVR"
     gene            1319..1543
                     /locus_tag="IXT20_002414"
     CDS             1319..1543
                     /locus_tag="IXT20_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4860837.1"
                     /translation="MNQRRRKRLEEISERLSELKDELEVIQEEEYKENIPKNLQGSER
                     YEKAEESVDQLEEAVDGLSDILELIEDIAS"
     gene            1546..1803
                     /locus_tag="IXT20_002415"
     CDS             1546..1803
                     /locus_tag="IXT20_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4860838.1"
                     /translation="MINEDFRSENKMIDISLIEKLLFSNKYTAKELATRWQISESAIK
                     AYRQNKGSVGWRDWKGISIGKAEDIMKREGFQADNDSKKVE"
     gene            2020..2895
                     /locus_tag="IXT20_002416"
     CDS             2020..2895
                     /locus_tag="IXT20_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4860839.1"
                     /translation="MTTNKRWSELVQNTVKKVMPDGYHFNYIQCSQTTESIYFSILNE
                     NRLYYFRVSSHHRKKREDNVTTFLSGKYNGFKELSEAIGEYLKSNRFVRISDKHILIL
                     LLYLNNSFKLFWRDDTFYYIENGMDIYSGINGILKHYIKALLRYGVLYQEDLSNQIKI
                     SNSGKSLLAKYKKLLSPIYKGIDMEAVNSETLFNLVVDEKFANEFFKSKKIERLNNLF
                     PENAIKAYVDNNKSRLYNWFIPKRLRGKIKIGDQVKVINLNHTKIVTVTELYIAEKED
                     ITKMKPVIKILDNKE"
     gene            2905..3699
                     /locus_tag="IXT20_002417"
     CDS             2905..3699
                     /locus_tag="IXT20_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4860840.1"
                     /translation="MVAKKNTLKEASEKYQDMKRRGLIPKDKKGIWREFVKEVSSDRS
                     GGDFVGRYTAKGYSRRLRITVEYGTNESATELKQRYLDTRREASKKYDDFKSRGLIPK
                     KKKGIWREFFKEEDSNRNKGDFIGRYTAKGYTKNLGIKVEYGTGESSWELRNRYLNTL
                     LEKYKAELLELFDVNDFIEMAEKYGHEEGFMINDIMVIDNFTDLLALAKKARDLRETQ
                     MYLFRNKNGRNRRTESSEIEDYYDAFTHDLGAFVDEISGVMNKYRV"
     gene            3728..4846
                     /locus_tag="IXT20_002418"
     CDS             3728..4846
                     /locus_tag="IXT20_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4860841.1"
                     /translation="MGNNSIYTPEMQNKIKRLVGLGWMTRGKLDSFSTIKYLLMDIYR
                     QNKQTGIHFLGYDKPEDAPFFVPSSAYRASTSGSMYNLDLFEEGAKMIEYDFNEAYSR
                     IMRNYPLPSNTYLKAKYNTDQAILRAINRPKVRPYENLRTYMFFLVNFEGTAKEGTYA
                     NPKSQSMFADYGENPKTANEPIWLSEIELTLILDYYDIKEMEVLDSHTFLTKKGLLKE
                     YFERIEPLKTDPDTLKLYKNLRTKVFGAIGQKSLREGETSTFDKYSMYNRAYSSMVAG
                     VFRDRMVRYEQKYVHSAYGLLKIRTDGLYFRSEVPEFEELYKLGVVKKKVHTISKEEV
                     EKALEFKNKGFENKGSFGKSKDEVKGVVIDDDFEFDFD"
     gene            4879..5199
                     /locus_tag="IXT20_002419"
     CDS             4879..5199
                     /locus_tag="IXT20_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4860842.1"
                     /translation="MKKKIVEGNVGVIILKDSTDYAKSMALKDEKVARMDKDLVEFLM
                     DDTERLKGDQFLSLMKDKGLNTYGLFDYKKLAIEWVKEGEIFFIEEWEGTENLVLHQY
                     LLKA"
     gene            5454..6143
                     /locus_tag="IXT20_002420"
     CDS             5454..6143
                     /locus_tag="IXT20_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum ABC transporter ATP-binding protein"
                     /protein_id="HAQ4860843.1"
                     /translation="MRTHYIYLEDKIVDEIPAKSREQARKLFIEKYKEINGDSEVPKV
                     VVTVRKRQTKKRSTFHVSPDIYNLIERHRSEDNISISQYIATVIDELISGKYDYAFDF
                     EGEKDYKSVAVFQSDFEKLKNYAGRRNVNFARLLEEFYSEELGVGNEYKRETYAKLSH
                     KLSQALKEVTEVKAELDAKLEEVKKYQTERKNNMKNQVEFDEQKEFEKARKRYNKIMN
                     ELSYEHLTVHE"
     gene            6180..6347
                     /locus_tag="IXT20_002421"
     CDS             6180..6347
                     /locus_tag="IXT20_002421"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4860844.1"
                     /translation="MVNEVETLLASFFENGHVNDEWRKEEPKRVNAYIARLKRFIDKY
                     KENSDKFSKDK"
     gene            6367..7995
                     /locus_tag="IXT20_002422"
     CDS             6367..7995
                     /locus_tag="IXT20_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4860845.1"
                     /translation="MPEIEGIFSPKIGKKQLDFSSFPSVNRDYNLGDEVQILNIDFKE
                     LDFAVAVSNKPKALERVKKIYQENDQNIKNMIKKYRRDNSKVEDVLFNGTAKHDLNVY
                     MALGVALYCNSINQTDILVNIVRNSYQKILKISSETNPYYAMKYLGDNLLNDKKLMHN
                     TEELLSNLAAINFLFKTGSEDYFIDNVLKGAYGTAFYPLYNMKQHKAEQKDNKLEIKK
                     MKELLGIGFVKPNSKVTIASLATTNNDGKIVNGFDDSDIDILSKSYFSLYGDFADLMG
                     IDSYLVNNFEIKGSELNDIVYFVLISLYAIDQNTEYRQIDYLLVFYSLLYQFVLTRLF
                     KNAKEELILNSDYEIAKQIASIRKENKSEVIKLRKELSIASKENGRLESLVKSAKKEE
                     ELLKQEIKELKGIRKELEQLRFENKVLKQLQEQEHRQSLNGKTEEPSLSEMIAYLKHK
                     NILLVGGHVIFQNKLTELLPNIKMFDVSENNGKILKNLNTCDAIFLYTDYLNHGLYYR
                     VMSEISNKDKIFYLDNHVNMNLTIKRIYDLLSLI"
     gene            8046..8417
                     /locus_tag="IXT20_002423"
     CDS             8046..8417
                     /locus_tag="IXT20_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4860846.1"
                     /translation="MRELTLSSKELEKKTALLNEIFSADCIIQSLKESSFSKLTDQEK
                     ISYMLSLDNLGTKYDESASSDKLLIELVSSLSSFGKMKRFNVILEIFAWLIPSFIWFF
                     YIFIVESELIAKVSLGLLIPI"
     gene            8502..8702
                     /locus_tag="IXT20_002424"
     CDS             8502..8702
                     /locus_tag="IXT20_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4860847.1"
                     /translation="MLKRIAWDKDNKTDIELKEYKYNYFSRCIDRKLLFKKEGLEFSF
                     DEDEFEKLVMDWRKNLLDTVQF"
     gene            8836..10062
                     /locus_tag="IXT20_002425"
     CDS             8836..10062
                     /locus_tag="IXT20_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4860848.1"
                     /translation="MIFYMFININEVLKLTTITMTKKIEVYVDGSQEERDSFFDFFWK
                     NSREYVKALNLVYKQLMFEELIKDAIKEMDQGYAEREESIQNRIEKLAYTDPIDQKKL
                     DKAKAELSKLRNEKNKEAREILTQAIGLKSQTRTRDVAKKLDFDFGDFVDSANMKASK
                     DFGNDFIGIVTGQRTPRIYKNRNKYGLLPFRGRDIKIHKDDKFYIYLPKGFRFKVNLG
                     SKPRKARDVASVLQHIMDGDYKLNQSNLIFDGRKLFFNLTISFEKSYNEMEFKKGNEL
                     IVNFADLCSGECLINDKSVMSFGDSQYIRNFKSKILAMKSKEQSRGVFSKGGHGRKRK
                     LDQDRWEAIKKRESNFVKTYNNQCSAKIVKTAIRNKAQIIRIIEPDKAYGEWAYYQLK
                     EQLVNKGKRYGIEVRS"
BASE COUNT         4083 a         1272 c         2106 g         3007 t
ORIGIN      
        1 aacaacgatt atgatcttaa aacaatcgtt tatgtaattg gcaatcttac gaataatttt
       61 catgcaataa tgacaataaa aaatgaaaag gggtaaaatc aaatgaaaaa atttttagta
      121 ggaattctat tcacaatggg gctagtatta ttatcacctt cagtttcagc agaaagctct
      181 atttccatgt atcgtatgta taatcccaat agcggagagc atctatacac tagaagcgtt
      241 ggagaaagag acaatctgaa acgttatgga tggcattacg aaggtgtcgc atggcaagct
      301 ccaacaagtg gtactcctgt ttatcgtttg tataatcggt ataacggtga acatttttac
      361 acagttgatg ccggagaaag agataagatt tcacacacag gatggacgta cgaaggtgtt
      421 gcgttctatt cctacaacgg agcaaataga gttcctgtaa cacgtttata taacaaatac
      481 gtgaattggc atcactacac gttagatcaa aatgaaaaga acgtaattag taaacaaggt
      541 tggaagattg aaggtacagg ctggtatgca atgccaaaca gcgtaccaaa tcctacacct
      601 gctccaagca ctgaaaaagt gttgaacgtt cctgttgtat ggcaaggaaa tacaatgtta
      661 tgcgaaggag cttctttatt agaaggttta cactacaaag gtgtagccac aaatcaaagc
      721 ttaaccagtt ttgtaaacta tatgccaaaa gctaatgaca ataatcctta tcatggttac
      781 tcaggagagt ggcgtcataa cgttaatgga acgtatcaag gcatgatggc tgacccagta
      841 gttaaatggg caaaaaacgt tggcggtaat gccgtcaata tcactggttc aggaacgaat
      901 ggaatcaaga atgaaattcg caatggtaat ccggttgtaa cttggattac ttataactat
      961 gctacgccag agttcaaaca aatgccttgg ggtcgtgcag tatggaatgg acatgtagtt
     1021 ctcgtggatg gcttcagaaa tggtgcatac catgttgttg accctgtatt tggcgcaaaa
     1081 tggatcaatg caagcacgta tgagagagca tttaatgtga ctggaatggc ggttgctgta
     1141 cgttaattga tagaattatc aactaaatga aataagagct taattactga aatggtaaat
     1201 aggctcttat ttttttagag gaaatttttg taatacgaat ctataacaaa aaatattgaa
     1261 tgtagtacac aaaagtggta tattaaaatc gtagagatta tgcgaagagg tgattttgat
     1321 gaaccaaaga agaagaaaaa ggttagaaga aataagcgag cgactttccg agctaaagga
     1381 tgagttagaa gtaattcaag aagaagagta caaagaaaat atacctaaaa atctacaggg
     1441 atcagaaaga tatgaaaaag cagaagaatc cgttgatcaa cttgaagaag ctgttgatgg
     1501 tttatccgac atattagaat tgattgaaga tatagctagc tagagatgat taatgaagat
     1561 ttcaggagtg agaataaaat gattgatatt tcactgatag agaagctact tttttcaaat
     1621 aagtacacag ccaaagagtt ggctacaaga tggcaaatat ccgaatcagc tattaaagcc
     1681 tatcgtcaaa ataagggaag cgtaggatgg cgtgattgga aaggaatcag cattggaaaa
     1741 gcagaagaca taatgaaacg tgaaggattt caagctgaca atgattctaa aaaagttgaa
     1801 tagtttaata gtcgccaaga aaagtaccag gacgagggga aaggtagatt acaaaggaaa
     1861 acacttttaa ataaacttcg caaaagcatc aggttatgag tggctagggc tatacgaatg
     1921 tgatgaaaac aaagggcttt aatgtcaaag aaaacgcaat agggggggaa caaaattttt
     1981 aaaaaagata tttatgtttg gtttaatgga aaaaaatata tgactactaa caaaaggtgg
     2041 tcagagctag tacaaaatac agttaaaaaa gtcatgccag atggatatca tttcaactat
     2101 atacaatgct cacaaacaac tgaaagtatt tatttttcaa tattaaatga gaatagactc
     2161 tattatttca gggtaagttc acaccatagg aaaaagagag aagataatgt tacgacattt
     2221 ttatcgggaa aatacaatgg cttcaaagag ttaagtgaag ccattggtga gtatctaaaa
     2281 agtaatagat ttgtcagaat ttctgacaaa catatattga tcttgttact ttatttgaat
     2341 aatagcttca aattgttttg gagagacgac actttctact atattgagaa cggcatggat
     2401 atttattctg gtatcaacgg catattaaaa cattatatta aggcgttact tcgatatggg
     2461 gttctttatc aagaagattt aagtaaccaa attaagatta gtaattctgg aaagtcatta
     2521 ctggcaaaat ataaaaaatt gctttctcct atctacaaag gaatagatat ggaagctgta
     2581 aattctgaaa ctttatttaa tttagttgtt gatgaaaagt ttgcgaatga attttttaaa
     2641 agtaaaaaaa ttgaaagact aaataacctg tttcctgaaa atgcaataaa agcttatgta
     2701 gataataata aatcacgatt atacaactgg tttattccca aacggttgcg aggaaaaatc
     2761 aaaattggcg atcaagtgaa agtaattaat ctaaatcata ctaagatcgt taccgtaacg
     2821 gagttgtata tagctgaaaa agaagatatt acaaaaatga aaccagttat taaaatacta
     2881 gataacaaag agtgagtgga aaatatggta gcaaaaaaga atactctaaa agaagcttct
     2941 gaaaaatatc aagatatgaa aagacgagga ttaataccaa aggacaaaaa aggtatatgg
     3001 agagaatttg ttaaggaagt cagtagtgat cgaagtggcg gtgactttgt tggacgatat
     3061 acagcaaaag gttatagcag gcgcttaagg atcactgttg aatatggaac gaatgaatca
     3121 gctacagagc tgaagcaaag gtacttagat acacgaagag aagcttcaaa aaaatatgat
     3181 gatttcaaga gcagaggtct aattcctaag aagaaaaagg ggatttggcg tgagtttttc
     3241 aaagaagaag atagcaatcg gaataaggga gacttcattg gacgatacac agcaaaaggc
     3301 tacactaaga atttagggat caaggttgaa tacggaactg gtgaaagttc atgggaattg
     3361 agaaatcgct atttaaatac attgttagag aaatataaag cagaattatt ggagttgttc
     3421 gatgttaatg attttatcga gatggctgaa aagtatggtc atgaagaagg ttttatgatc
     3481 aatgacatta tggttattga taattttacg gatttattag cacttgcgaa aaaagcaagg
     3541 gatttaagag aaacgcagat gtatctattc agaaataaaa acggaagaaa ccggagaact
     3601 gaatcgagtg agattgaaga ttactatgat gcctttacac acgatctggg agcctttgta
     3661 gacgagataa gtggtgtaat gaacaaatat agggtctaga ataaaaaagc cggagatgag
     3721 cagataaatg ggaaacaata gcatatacac acctgaaatg cagaataaaa ttaaaagact
     3781 tgtcggatta gggtggatga caagaggaaa attagattct tttagtacga tcaaatatct
     3841 tttgatggac atttaccgtc agaacaagca aacaggaata cattttttgg gctatgataa
     3901 gccggaagat gcacctttct ttgttccaag ttcagcctat agagccagta cctccggtag
     3961 catgtataac ttggatttgt ttgaggaagg agcaaagatg atcgaatatg atttcaacga
     4021 agcctattcg aggatcatga gaaattatcc tttgccgtca aacacttatt tgaaagcaaa
     4081 atataataca gatcaagcta ttttgagagc gataaataga ccaaaagtaa gaccgtatga
     4141 aaatttaaga acgtatatgt tctttttggt taattttgaa ggaacagcca aagaaggaac
     4201 atatgctaat ccaaaatcac agtcgatgtt tgcggattat ggcgaaaatc ccaaaacagc
     4261 taatgagcct atttggttat cggagattga gctaacactt atccttgatt attatgatat
     4321 taaagagatg gaagtcctag atagccacac atttttgacg aaaaaaggat tactcaaaga
     4381 atattttgaa agaatcgagc cattaaaaac tgatcctgat acgttgaagc tttataaaaa
     4441 tcttagaacg aaagtatttg gtgctatcgg acaaaaatca ttaagagaag gagaaacaag
     4501 caccttcgat aaatacagta tgtataatcg agcgtattcc tccatggtag caggcgtatt
     4561 tagagatcgt atggttagat atgaacagaa atacgttcat agcgcatatg ggctattaaa
     4621 aattcgtact gatggattgt atttcagatc cgaagtacca gagtttgaag agctgtataa
     4681 acttggagta gttaagaaaa aagttcatac aatatccaaa gaagaagtcg aaaaagcttt
     4741 agaatttaaa aacaaaggat ttgaaaataa gggctccttt gggaaatcta aagatgaagt
     4801 taagggtgtt gtgatagacg atgatttcga gtttgatttt gactaaatac tctttggaaa
     4861 agatggtagg aggtaaatat gaagaagaaa attgtggaag gaaatgtagg tgttattatt
     4921 ctaaaagatt caactgacta tgctaaatca atggcactaa aagacgaaaa agttgcacgc
     4981 atggataaag atttagttga atttctgatg gatgatacag aaagattaaa aggggatcag
     5041 tttttgagtt taatgaaaga caaaggctta aacacatatg gtttatttga ttataagaaa
     5101 ttagctatag agtgggtaaa agaaggagaa atatttttta ttgaagaatg ggaaggtact
     5161 gagaacttag ttcttcatca gtatcttttg aaagcttaat ctaagtaaaa cagttctgac
     5221 tagttatgct tgtcagaatt tcaaaggaag ttttccataa ccatcatcat tcaaaaataa
     5281 aggatttgaa aataagggtt cctttgggaa atctaaagat gaagttaagg gtgttgtgat
     5341 agacgatgat ttcaagtttg attttgacta gatttttttg caatgtttaa gagtaaacaa
     5401 ggtaagaact aagatcatga agattgagag taaagaagat agaggacgaa tgtatgagaa
     5461 cacactatat ttatttagaa gataaaattg tagatgaaat tccggcaaaa agtcgagaac
     5521 aagctagaaa attatttatt gaaaaataca aagagatcaa tggagatagt gaagttccaa
     5581 aagtggtcgt aacagttaga aaaaggcaaa caaaaaagag aagtacgttt catgtcagcc
     5641 ctgatatata taatcttata gagcgacata gaagtgaaga taatatttct atctctcaat
     5701 atattgctac agttattgat gaattgattt caggaaagta tgactatgct tttgattttg
     5761 agggtgaaaa ggattacaaa agtgttgctg tctttcaatc tgattttgag aagctaaaaa
     5821 actatgctgg caggagaaat gttaattttg caagactact agaagagttc tactccgaag
     5881 agttgggagt aggaaatgaa tacaagagag aaacttatgc gaaattgtct cataagttat
     5941 cccaagcgct taaagaagtg acagaagtta aagcggaact agatgctaaa cttgaagaag
     6001 tgaaaaaata tcaaacagag aggaagaaca acatgaagaa tcaagtggaa tttgacgaac
     6061 agaaagaatt tgaaaaagca agaaaacgct ataacaaaat tatgaatgaa cttagctatg
     6121 aacacttaac agttcatgaa taattgagtg agggtacaga aaaatatacg atcgcagata
     6181 tggttaatga agtggaaact cttttagctt ctttctttga aaatggtcac gtaaacgatg
     6241 aatggcgaaa agaagaacct aaaagggtta acgcttatat tgctcgatta aaaaggttta
     6301 ttgataaata taaggagaat agtgacaaat tttctaaaga taaataagga gagagtgtga
     6361 ttacatttgc cagaaatcga aggaatattt tcgccaaaaa taggtaaaaa acagttagat
     6421 ttcagcagtt ttccgagcgt aaatagagat tataatctag gtgatgaggt acagatacta
     6481 aatattgatt tcaaagaact agacttcgct gttgctgttt ccaacaaacc caaggcattg
     6541 gaacgagtga aaaaaatata ccaagagaat gatcaaaata tcaaaaatat gatcaaaaag
     6601 tatagacgag ataattcaaa agttgaagac gttttattca acggtacggc aaaacatgat
     6661 ttgaatgttt atatggcatt aggagtggct ttgtactgta attcaattaa tcagacagat
     6721 attttggtta atatcgtgag gaatagctat caaaaaatat taaaaataag tagcgaaaca
     6781 aacccttatt atgcaatgaa atatcttggg gataatttat taaatgataa aaaattgatg
     6841 cacaacactg aagagctgtt atctaatttg gctgcgatta actttctatt caaaacaggt
     6901 tctgaagatt attttataga taacgtacta aaaggtgctt acggaacagc attttatccg
     6961 ctttacaata tgaaacagca taaagccgaa caaaaggata acaaacttga aattaaaaag
     7021 atgaaagaac tattaggtat aggatttgta aaacccaatt caaaagtcac tattgcttcg
     7081 ttagcaacta ctaacaatga tgggaaaata gtaaatgggt ttgacgattc agatatagat
     7141 atattgtcaa aatcatattt ttctttgtat ggagattttg cagatttgat gggtattgat
     7201 agttacttag tcaataattt tgagatcaag ggtagcgagc tgaatgatat tgtgtatttc
     7261 gttttaatct ctctatatgc aattgatcag aataccgagt accgacaaat tgactatttg
     7321 ctagtttttt atagtttgtt atatcagttc gttttaactc ggttgtttaa aaatgctaag
     7381 gaagaattga tcctgaattc tgattatgaa atagcaaaac aaattgcaag tattagaaaa
     7441 gaaaacaaat ctgaagtaat caaattaaga aaagaattaa gtattgcgag taaagaaaac
     7501 ggtcggttag aatctttagt caaatcggca aaaaaagaag aggaactttt gaagcaagag
     7561 attaaagaat taaaaggaat cagaaaagaa cttgaacagc tgaggtttga aaataaagta
     7621 ttaaagcaat tgcaggagca agaacataga caatcattaa atggtaaaac tgaagagcct
     7681 tcactgtctg aaatgatagc ttatctaaaa cataaaaaca ttcttttagt gggaggacat
     7741 gtaatttttc aaaataaatt aactgaacta ttaccaaata ttaaaatgtt tgatgtaagc
     7801 gaaaataatg gaaagatact taaaaatttg aatacgtgtg atgcgatttt tctatataca
     7861 gattacttga atcatggtct atattatcgt gttatgagtg aaatttctaa taaagataag
     7921 atattttatt tggataatca tgtgaatatg aacttaacaa ttaaacgtat ttacgatctt
     7981 ttatcactta tataaagaga gaagtgataa ataggacaca aaatttggaa agtaggtaag
     8041 tcactatgag agaactaact ttatcttcta aggaattaga aaagaaaact gctttattaa
     8101 acgaaatatt tagtgctgat tgtataatcc aaagtttaaa agaaagttca ttttcaaaac
     8161 taacagatca ggaaaaaatt tcttatatgc taagtttaga taatctagga actaaatatg
     8221 atgaatcagc aagtagcgat aagcttttga ttgaattagt atcaagtctt agtagttttg
     8281 gaaaaatgaa aagatttaat gtgatattag aaatttttgc ttggttaatc ccttcattta
     8341 tttggttttt ttatattttc atagttgaat ctgagttaat agccaaggtc tctcttggac
     8401 tgttaattcc gatataaata ggttcagcaa tttactttaa taacttgtat acacgtattc
     8461 aaataaaaaa gcagttatta catgaaggat taagtggtaa tatgttaaaa agaattgcat
     8521 gggataaaga taataaaaca gatattgagc ttaaagaata taaatacaat tatttctcga
     8581 gatgtataga tagaaagtta ctgtttaaaa aagaaggatt agaatttagt tttgatgaag
     8641 atgaatttga aaaattagtg atggactggc gtaagaattt actggatacg gtacaattct
     8701 aaaagcaatt gaggtcgaaa ttccgatact tgaatcatga tctatattat cgtgttatga
     8761 gtgaaatttc taataaagat aagatatttc atttggacag tcatgtgaat atgagcttaa
     8821 caattaaacg tatttatgat cttttatatg tttataaaca ttaatgaggt gctaaaattg
     8881 acaacaataa ctatgacaaa aaaaatagaa gtatatgttg atggatcaca agaagaaagg
     8941 gattcctttt ttgatttttt ttggaaaaat agtagagaat atgtaaaagc gttgaatctg
     9001 gtatataaac agctaatgtt tgaagagttg ataaaagatg caattaaaga aatggatcaa
     9061 ggttatgctg agagagaaga aagtattcaa aatcggattg aaaagttagc atatactgat
     9121 ccaatagatc aaaaaaaact tgataaagca aaagctgaat tatctaaatt gagaaatgaa
     9181 aaaaataaag aagcgagaga aatattaacg caagcgatag ggttgaagag tcaaacgaga
     9241 acacgagatg tagcaaaaaa attagacttt gactttggag attttgttga ttccgcaaat
     9301 atgaaagcta gtaaagattt tggcaatgat tttatcggaa ttgtgaccgg acaaagaacg
     9361 cctagaatat ataaaaatag aaacaaatat ggattactac cttttagagg tcgagatatt
     9421 aaaatccata aagatgataa gttttatatt tatttaccta aaggctttag gtttaaagtg
     9481 aatttaggta gcaagccaag aaaagcaaga gatgttgctt cagttttaca acacattatg
     9541 gatggcgatt acaaattaaa tcaaagtaat cttatttttg atggaagaaa attatttttt
     9601 aatctaacaa tttcttttga aaaaagctat aatgaaatgg aattcaagaa aggtaatgaa
     9661 ttgatcgtaa actttgcaga tttgtgtagc ggtgaatgtt taattaatga taaatctgta
     9721 atgtcttttg gtgatagcca atatataaga aacttcaaaa gtaaaatttt agcaatgaaa
     9781 tcaaaagaac aaagccgagg agtcttttct aagggcggtc atggtcgaaa aagaaaatta
     9841 gatcaagatc gatgggaagc aataaagaaa agagaaagta attttgttaa aacttataat
     9901 aatcaatgtt ctgcaaaaat cgttaaaact gctattcgca ataaagctca aattattcgt
     9961 atcattgaac cagataaggc ttatggcgaa tgggcttact atcaattgaa agaacaacta
    10021 gtaaacaaag gaaaaagata tgggatagaa gtcagaagtt agtaaattaa acaatcgttg
    10081 atttggcaac gaaatagtga gggaaaatcc actcactgaa gggtaaactc cgtcaagttg
    10141 tgtgaccaga cggaaggacg aatgttgttg aggttttaga accatataaa acagcatgtt
    10201 tctcaaattt ttaggataag ggaaattgct agcagtttgt tttagaacca tataaaacag
    10261 catgtttctc aaatgagtac tttaaattaa ttgatggcgt tcctgcttta gaaccatata
    10321 aaacaacatg gatttaaaac aagaagtaag tcagttctcc ttttacttta gtaaggaaaa
    10381 ataaagtatt ctcaataaaa cttaaaataa gcaaaccttt gttttaacag aattaggcag
    10441 aagtcgccca tcctctatag gtgggtga
//
LOCUS       DACGLE010000089        10268 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0534
            SAMEA3304449-rid9560453.denovo.094, whole genome shotgun sequence.
ACCESSION   DACGLE010000089 DACGLE010000000
VERSION     DACGLE010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304449
            Sequence Read Archive: ERR985064
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10268)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10268)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702225.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702225.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 20:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,045
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 27 of 67
            Pseudo Genes (incomplete)         :: 25 of 67
            Pseudo Genes (internal stop)      :: 19 of 67
            Pseudo Genes (multiple problems)  :: 4 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10268
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304449-rid9560453.denovo.094"
                     /strain="VREN0534"
                     /db_xref="taxon:1352"
     gene            28..3117
                     /locus_tag="IXT20_002426"
     CDS             28..3117
                     /locus_tag="IXT20_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002389909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HAQ4860849.1"
                     /translation="MSLFYLKEKLEARIEELGKYRFSKIVELSDWLVVEDETKKAKYP
                     PKDFSSAATFSVGDTWKGRDFYLWVKKEIEMPDVDDLYLYLNFGHTGGGYNSGFESLM
                     FINGKPYQGVDSNHKEVKIADEFRGKKVEVALKLWSGLEGGGPEQIQVHQFKQAELSQ
                     MMVPVDELFYYADAINKTVKELGQDNPIQYKLSHVLNHAINQINWIEPGSQTFYTSVA
                     KAVSELSEGIAAIPKDEMITVTAVGHTHIDVAWLWRLKHTREKAARSFSTVLRLMEEY
                     PEYIFLQTQPQLYKYIKEDYPEIYAKIKERVAEGRWEADGSMWLEADCNIPSGESLTR
                     QILHGAKFIKEEFNQDIHYLWLPDVFGYSWALPQILTKSGIDTFMTTKISWNQFNRMP
                     HDTFKWKGMDGSEVLTHFITTPEPGKENSWESNWYYTYNGELEPETVLGVYNAYRDKN
                     INDELLISYGFGDGGGGVNRDMLEKRRKLDKIPGLPKVQTGKAVDYFDRLQETFKNTD
                     EYVHTWDGELYLEYHRGTYTSQAYVKKANRQSELLLRQLEILFSLMEMKGQEAYPKVE
                     IFNMWETVLRNQFHDIIPGSSIKEVYQDHRIEMEGLFKEAQSLLAPLQSVGDSQTVCN
                     FANWQRSILAELPFLLDNQVYVDEAGEVIETYANGEVLYGLISNVPALGQKMIHVTEK
                     QEMAQIPTVAVVDSNQIETDFYLIQWNKSGQLTSVFDKELKREVLSGTGNVLQLFEDK
                     PMNFDAWDIDIYYQEKFQELAAQEIIVKPANALYQCVEFIYRFGESTIKQEMRLYPHT
                     RRIDFVTDIDWQERQQLLKAKFDVAVRATYATYDIQYGNVRRANNWNTSWEMAKFETV
                     AHQWMDMSQRDFGVSILNDSKYGCDVKDQTMRLSLLKGAIHPDPTADMGQHHFTYSLY
                     PHRGDFIEGKTIQEAWEINEKPLVLNGSIGTLPQITTTQDATIVIDTIKKAEDGNGWI
                     IRAHEYAGTDQTVDLTIEGLTWWRETDLLERNIGEVKDKQPSLTFAPYEIKTIRFGK"
     gene            3086..4453
                     /locus_tag="IXT20_002427"
     CDS             3086..4453
                     /locus_tag="IXT20_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HAQ4860850.1"
                     /translation="MKLKQFVLGNEGRALEITAEFCQKLKSNYEGTPKYKLAELGVVR
                     NGVVESAENVSAKSQNPFVFSIEVPTLKIPQQYHSGRCWLFAIVNLLNQQISQKLEIA
                     EFELSQNYLYFYDKLEKANFFYKNIIETADLELGSRELDFLLSKPQEDGGQWDMLVSL
                     IEKYGIVPSYSMPETENSRSSADMNKYLNAKLRLDAKKLRQMVAEDCSQKDIDEVTSQ
                     MLAEVYRMLAICIGAPPEKIDMKYRDDAGNYHAGLNLTPKEFANKYLLMNLKDYIPII
                     HAPSKNKVYNQTYTVAMMGNIIEGQPIKYLNVDMATLKALTIKQLKDHEAVWFGSDIG
                     QYTDRKLGIMATDIYAVDNLFDVDFTSNKQDRLEYCQSLLTHAMVISGVDLQNDQPTI
                     WKVQNSWGEEIGYKGFFMMTDAWLEEYVYQVVINKKYLTKEQLAKYQEEPVILPPWDP
                     MGSLA"
     gene            4528..5346
                     /gene="iolB"
                     /locus_tag="IXT20_002428"
     CDS             4528..5346
                     /gene="iolB"
                     /locus_tag="IXT20_002428"
                     /EC_number="5.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002389912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-deoxy-glucuronate isomerase"
                     /protein_id="HAQ4860851.1"
                     /translation="MNLLQKPILFPVNEKVQLIQYITGENSVLEHVELSVYDVEAGGE
                     VSYHEAALESCVVVLSGQVSVAILNGETYPKLGTRTSVFDKIPTHSVYIGKNRSYKVT
                     AETKARILIAASKTDQSLPDRLIKPEDVLLESRGAYQNKRYVQTILSDQDTISDKLLV
                     VEVYTEGGNFSSYPPHKHDQNRLPEESFLEETYYHEINPPQGFVFQRIYSDDRLLDQT
                     MSCSNQDVVIVPKGYHPVGVPDGYTSYYLNIMAGPTKMWRFYDDPDHEWIKNRS"
     gene            5396..6373
                     /gene="iolC"
                     /locus_tag="IXT20_002429"
     CDS             5396..6373
                     /gene="iolC"
                     /locus_tag="IXT20_002429"
                     /EC_number="2.7.1.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010766229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-2-deoxygluconokinase"
                     /protein_id="HAQ4860852.1"
                     /translation="MKKFAITALGRACIDLNSVEINQPMEETKTFKKYVGGSPANIAI
                     GLAKLGETVGFIGKLSNDQHGRFIKDFMSKAGVDSSQMIFDQEGHKSGLAFTEIKSPS
                     ECSILMYREDVADLYLTPDEVSEEYIEASEMLLISGTALAQSPSREAALLAVEYAHKH
                     DVKVIFELDYRPYTWGSTAETAVYYTLLAQKAHIVIGTRDEFDVLENSVGGNNQQTVE
                     RLFQYTPELILIKHGVDGSFAYEKDGTITRGYAYKAQVLKTFGAGDSYAAAFLFALMQ
                     GKMIENALQYASASSALVVSRHSSSEAMPSVSEIEAVIAEQSWGEKVHA"
     gene            6366..8282
                     /gene="iolD"
                     /locus_tag="IXT20_002430"
     CDS             6366..8282
                     /gene="iolD"
                     /locus_tag="IXT20_002430"
                     /EC_number="3.7.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_391852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3D-(3,5/4)-trihydroxycyclohexane-1,2-dione
                     acylhydrolase (decyclizing)"
                     /protein_id="HAQ4860853.1"
                     /translation="MPKTLRLTTAQALVKYLNQQFLIVDDQKLPFVKGIFHIFGHGNV
                     LGLGQALSQNPGHLEIYQGKNEQGMGQAAVSFAKEKLRRQIFAVTTSVGPGAANLVTV
                     AGTALANNLPVLLLPGDTFASRQPDPVLQQVEHFHGQGITTNDALKPLSRYWDRIERP
                     EQLMSALTRAFEVLTDPVTAGPVTICLPQDVEGEAYDYPEDFFNEKVHYFDRRLATDR
                     ELSEAVKLIERSLKPVIIVGGGAKYSEAGSALKRFSEKHKIPLVETQAGKSTVVADFP
                     LNFGGVGVTGGAPANRLVKEADLVIGLGTRYTDFTTASKTAFDKDQTTFLNINVSRMQ
                     SYKMNGVSVVADAKTALLQIESELDDLYQTQYGDLLTSVTRDWQNERERLANLNYHDA
                     DFEMEIKNHFGAEKLANYAQQLTTELTQTAALIKINQSLPLDSIVVGAAGSLPGDMQR
                     LWKAKTVNSYNMEYGYSTMGYEVAGAYGAKIAHPDKEVFAFVGDGSFHMLHSELLSSI
                     QYGKKINVLLFDNSGYGCINNLQMGNGMPSFGTELTDYHEEIMTVDFAKIAEAYGAVA
                     YNVGNMSELEKSLAEIAESDRSTLIHIKVLPKTMTDGYDSWWNTGIAEVTADGSTEKV
                     LAEKEEKLAGAFQY"
     gene            8304..9458
                     /locus_tag="IXT20_002431"
     CDS             8304..9458
                     /locus_tag="IXT20_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011887560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HAQ4860854.1"
                     /translation="MYQLTLPQKIIYGDQAIKETGKIAGNLGLKTLIITDKLIVSLNL
                     LEGVLASLKEENLTWKIFDGVDREPTDQIVAEALAFYQQEDYDSIIAVGGGSCIDTAK
                     AVAVLINNSGSYAEFASGQLIAQNPLPLIAIPTTAGTGSEVTDVTVITDTQKDIKYMM
                     KQAAFLSYAAIVDPQMSRTSPASVTAATGLDALCHALESYISKKAQPITRLFSLSAIK
                     LIMENLPIAYSEPENLIARENLAQAAMEAGIAFSNASVTLIHGMSRPVGALYHIPHGI
                     SNAILLEAVIDYTKPVIISELAGIAKYLNPEKEGTDQELAECFFQELQDLIEKLAVPN
                     YASFGIEKAAFIASLDKMANDAIASGSPNNNPLVPTIEEIKMIYLASWQE"
     gene            9481..9912
                     /locus_tag="IXT20_002432"
     CDS             9481..9912
                     /locus_tag="IXT20_002432"
                     /inference="COORDINATES: protein motif:HMM:NF012792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ4860855.1"
                     /translation="MEIKTDLNSEDSSQLAEFLLMVLDQQQEHFFPDYQTIDKSVALA
                     AYSGTELVGGLLGKISDQSLNVSLLAIDPKHQKDGLGSQLIEEVEKIAAKNQVINLLL
                     STRSYQALAFYQKNNFEIYGELKDMPFRGVDTYYLVKRLMA"
     gene            9950..>10268
                     /locus_tag="IXT20_002433"
     CDS             9950..>10268
                     /locus_tag="IXT20_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 125 protein"
                     /protein_id="HAQ4860856.1"
                     /translation="MTYKEIPVSVQQFMEKITTLCGNDHPQWAANINAAFANTLLTTV
                     KRHEDGTTFLLTGDIPAMWLRDSTAQVRPYLVIAKDNPDLADMISGLVKKQFFYINLD
                     PYAN"
BASE COUNT         3236 a         1796 c         2323 g         2913 t
ORIGIN      
        1 aaaatgaaaa gggtggagga atagttcatg agtttgtttt acttgaaaga aaaattagaa
       61 gcaagaatcg aagagttagg aaaatatcgc ttttcaaaaa tagtagagtt gtctgattgg
      121 cttgttgtag aagatgagac caaaaaagca aaatatccac ccaaagattt ttcaagtgct
      181 gctacttttt cagtagggga tacttggaaa ggccgcgatt tttatttgtg ggttaaaaaa
      241 gaaatcgaga tgccagatgt tgacgattta tatttgtatt tgaattttgg tcataccggt
      301 ggtggttaca actccggctt tgaatcattg atgttcatta atggaaaacc ctatcaaggc
      361 gtggattcca atcataagga agttaaaatc gccgatgaat tccgtggtaa aaaagtcgag
      421 gtagcattga agctttggtc aggtttggaa ggtggcggac cggaacaaat ccaagtccat
      481 caatttaagc aagcggagct atctcaaatg atggtgccag ttgatgaact attttattac
      541 gctgatgcta tcaataagac cgtgaaagaa ttaggtcaag ataacccgat tcaatacaag
      601 ttgtcccatg ttttaaatca tgctatcaac cagattaatt ggatcgaacc tggaagccaa
      661 acattttata cttcggtagc gaaggctgta tccgaattat cagaggggat tgccgcaatc
      721 cctaaagatg aaatgatcac tgtgacagca gtcgggcata cccatattga cgtagcgtgg
      781 ttatggcgat taaaacatac ccgcgaaaaa gctgcccgta gcttctcaac tgttttacgt
      841 ttgatggagg agtatccgga gtatattttc ttacaaacac aaccgcaatt atacaaatat
      901 atcaaagaag actatccaga aatttatgca aaaatcaaag aacgagtagc tgaaggtcgt
      961 tgggaagcgg atggaagcat gtggttggag gcggattgta acattccttc tggtgaatct
     1021 ttgacccgcc agattttaca tggtgcgaaa tttattaaag aagaattcaa tcaagacatt
     1081 cactatttgt ggttgccgga cgtttttggc tattcttggg cattgcctca gattttgact
     1141 aaaagtggca ttgatacttt tatgacgaca aagattagtt ggaatcagtt taatcggatg
     1201 cctcatgata ccttcaaatg gaaagggatg gatggctcag aagtcttgac ccattttatt
     1261 accacgccag aaccaggcaa agagaattca tgggaatcca attggtacta tacctataat
     1321 ggtgagttag aacctgaaac tgttttaggc gtttacaatg cttatcgaga taaaaacatt
     1381 aatgatgagt tgttgatttc ttatggattt ggcgatggcg gaggcggcgt taatcgtgat
     1441 atgttggaaa aacggcggaa attagacaaa attcctggct taccgaaagt tcaaaccggt
     1501 aaagcagtgg attacttcga tcggctgcaa gagaccttta aaaatactga tgaatatgtt
     1561 catacctggg atggcgaatt gtatttagaa tatcatcgag gcacctatac ctctcaagct
     1621 tatgtcaaaa aagcgaaccg ccaaagtgaa ttattgttgc gccagttaga aatcctcttt
     1681 tcattaatgg aaatgaaggg acaagaggcc tatcccaaag ttgaaatctt taatatgtgg
     1741 gaaacggttt tgcgtaatca attccacgat attatcccgg gatcttcaat taaagaggtt
     1801 tatcaagatc atcggattga aatggaagga ctttttaaag aagcccaaag tttattagca
     1861 ccattgcagt cagtaggtga cagccaaaca gtttgtaact ttgcaaactg gcagcgttca
     1921 attttagctg aattaccatt tcttttggat aatcaggttt atgttgatga agctggtgag
     1981 gtaattgaaa catacgctaa tggtgaagtg ctttatggat taatttcaaa tgttccagct
     2041 cttggtcaaa aaatgattca tgtcactgag aagcaggaaa tggcgcagat tcctacagta
     2101 gctgttgtgg attcaaatca aattgagaca gacttttatt tgatccagtg gaataaaagc
     2161 ggacaattga cttcggtatt cgataaggag ttaaaacgag aagttttaag cggaaccggg
     2221 aatgtcctgc aattatttga agataaaccg atgaattttg atgcttggga tatcgatatt
     2281 tattatcaag aaaaattcca agaactagca gcacaagaaa ttatcgtcaa acccgctaat
     2341 gctttatatc aatgtgtcga atttatttat cgttttggtg aatcaaccat taaacaagag
     2401 atgagattat atcctcatac tcgtcgcatt gattttgtga cagatattga ttggcaagag
     2461 cggcaacagc tgctaaaagc taaattcgat gttgctgtca gagcaactta cgcaacttat
     2521 gatattcagt acgggaatgt tcgtcgtgcc aataactgga atacgagctg ggaaatggct
     2581 aaattcgaga ctgtcgccca tcagtggatg gatatgtcac aacgtgattt tggggtcagc
     2641 atcttaaatg attccaaata tggttgcgac gttaaggacc aaacaatgcg tttgagtttg
     2701 ttgaaaggag caattcatcc tgatccaacg gcggatatgg gccaacacca ttttacctac
     2761 agtctgtatc cacatcgtgg cgactttatt gaaggaaaga ccattcaaga agcttgggaa
     2821 atcaatgaaa aaccgcttgt tttaaatggt agtattggca ctttaccgca aattaccaca
     2881 actcaggatg cgactatcgt aattgatacg attaaaaaag ccgaagatgg taatggttgg
     2941 attatccggg cccatgaata tgccgggact gatcagacgg ttgacctgac aattgaaggt
     3001 ttaacttggt ggcgtgagac ggacctgttg gaaagaaata ttggcgaagt aaaagataaa
     3061 caaccgagtt tgacatttgc accatatgaa attaaaacaa ttcgttttgg gaaatgaggg
     3121 aagagcattg gaaataactg cagaattttg tcaaaagtta aaaagtaact acgaaggaac
     3181 ccccaaatat aaactagcag aattaggtgt tgttcgaaat ggtgttgttg aatcggcaga
     3241 aaatgtctca gctaaaagtc agaatccctt tgtgttttca atcgaagtac cgaccttaaa
     3301 aattcctcag caatatcatt cgggacgctg ttggctgttt gcgattgtca atttactgaa
     3361 tcagcaaatc agtcagaagt tggaaatcgc tgaatttgaa ctttcgcaaa attatctata
     3421 tttttatgac aagttagaga aagccaactt tttctataag aatattattg agacggctga
     3481 tttagagttg ggcagtcgtg aattagattt tctactatct aagccccaag aggatggtgg
     3541 tcaatgggac atgctagttt cattgattga aaagtatggg attgttccat cttattcgat
     3601 gccggaaacc gaaaatagtc gcagttcagc tgacatgaac aagtatctaa atgccaagtt
     3661 gcgacttgat gcaaaaaaac tgcgtcaaat ggtggctgag gattgttcac aaaaagacat
     3721 tgatgaagtg acttcccaaa tgctcgcgga agtttaccga atgttggcaa tctgtatcgg
     3781 ggctccgccg gaaaagattg atatgaagta tcgtgatgat gctggaaatt atcacgctgg
     3841 attaaatcta acaccaaaag aatttgccaa taagtatttg ttaatgaatt taaaggatta
     3901 tattccgatc attcatgcac cttccaaaaa caaagtgtac aaccagacct acacagtagc
     3961 gatgatggga aatattatcg aaggacaacc gattaaatat cttaatgtag atatggcaac
     4021 gttaaaagca ttgacaatca agcaactgaa agatcatgaa gccgtctggt ttggtagtga
     4081 tatcggccaa tatactgacc gaaagctagg gattatggcc acagacatct atgctgttga
     4141 taatttattc gatgtcgatt ttacttcaaa taaacaggac cgtttagaat actgtcaaag
     4201 tcttttgact catgccatgg taatcagcgg agtagatttg caaaatgatc agccgacgat
     4261 ttggaaagtc cagaatagct ggggcgaaga aatcggttat aaaggctttt tcatgatgac
     4321 cgatgcttgg ctagaggaat atgtctatca ggtggtgatt aacaaaaaat atttaacaaa
     4381 agagcaactg gcaaagtatc aagaggaacc agttatttta ccaccctggg atccgatggg
     4441 ttctttagca taataaaaaa accgcggcta gtcataacga gatgattgtt tgtgaccggc
     4501 ccccgatttt agtaaagagg gaaaataatg aatcttttac aaaaaccaat tttatttcca
     4561 gtgaacgaaa aagttcagtt gattcaatat ataaccggag agaatagcgt ccttgagcat
     4621 gttgaattaa gtgtttacga tgtcgaagct ggcggtgagg ttagttacca tgaagctgct
     4681 cttgaaagtt gcgttgttgt tctttccggc caagtgtccg tagcgatttt aaatggagaa
     4741 acctacccta agcttggaac gagaacttca gtttttgata aaataccgac tcacagtgtt
     4801 tatattggta aaaatcgttc atacaaagtt acggcagaaa cgaaagcgcg tattttgatt
     4861 gctgcttcta aaacggatca aagtctacca gatagattga tcaagcctga ggatgtctta
     4921 ttggaatcac ggggtgctta ccaaaataaa cgctatgtgc agacgatctt gtctgatcaa
     4981 gatacaatca gcgataaact tttagtcgtg gaagtttaca cagaaggtgg taatttttct
     5041 agctatccac ctcataaaca cgatcaaaac cgcctaccgg aagagtcttt cttagaagaa
     5101 acttattatc atgaaatcaa tccaccacaa gggtttgttt ttcaacggat ttattccgat
     5161 gatcgtcttt tagatcaaac catgtcctgt tccaatcaag acgtggtaat cgttccaaaa
     5221 ggctatcatc ctgttggcgt accagatggg tacacttctt attatctaaa tatcatggcc
     5281 ggtccaacga aaatgtggcg tttctatgat gatcctgatc acgaatggat caagaaccga
     5341 tcgtaagagt ttttcagaaa aataaaacgt gtcatgagag aggaagaaaa ttaaaatgaa
     5401 gaaatttgct attacagctt tagggagagc ctgtattgat ttgaactcag tagaaattaa
     5461 ccaaccaatg gaagaaacaa aaacatttaa aaaatatgtt gggggatctc cggcaaatat
     5521 tgcgattggt ttggccaagt taggggaaac agtaggtttc atcggaaaac tttctaatga
     5581 tcagcacggt cgatttatta aagacttcat gtcaaaagcg ggtgttgatt caagtcagat
     5641 gatttttgat caggaaggac ataaatccgg actggctttt accgaaatta agagtccatc
     5701 agaatgcagt attttgatgt atcgtgaaga tgtggctgac ttatatctaa cccctgatga
     5761 agtttcggaa gagtacattg aagccagcga aatgctattg atttcaggaa cagctttagc
     5821 ccaaagtccg tcaagagaag cagcgttatt ggcggtggaa tatgcccaca aacatgatgt
     5881 aaaagtgatt tttgaattgg attatcgtcc ttatacgtgg ggatcaactg ctgaaacagc
     5941 cgtttactac actttgctag cacaaaaagc ccacattgtg attggtaccc gcgatgaatt
     6001 tgatgtgtta gaaaatagtg ttgggggtaa caaccaacaa actgttgaaa gacttttcca
     6061 atatacgcct gaactgatcc tgattaaaca cggggtcgat ggctcatttg cttatgaaaa
     6121 agatggcacg attacgcgag gatatgccta taaagcacag gttttgaaaa cctttggggc
     6181 aggtgattct tatgcggccg ctttcttgtt tgctttgatg caaggaaaaa tgattgagaa
     6241 tgctttacag tacgctagtg cctcgtcagc gctagtagtt agtcgtcata gttcttcgga
     6301 agctatgcca agtgtgtccg aaattgaggc agtcattgca gaacaaagct ggggggagaa
     6361 agttcatgcc taaaacgcta cgcttaacca cggcccaggc attggttaag tatttgaatc
     6421 aacagttttt gattgtagac gatcaaaagc taccctttgt gaaaggtatt ttccatatct
     6481 ttggccacgg aaatgtctta ggattgggac aagctttgtc acaaaatcct ggtcatttgg
     6541 aaatctatca aggaaaaaat gaacagggaa tgggccaggc ggctgtttcc tttgcaaaag
     6601 aaaaactgcg ccgtcaaatt tttgcagtaa cgacttcagt gggaccgggt gctgcgaatc
     6661 ttgttacagt agctggcact gccttggcta ataatttgcc agtattatta ttgccgggag
     6721 atacttttgc aagccgtcaa ccagatccag tcctgcagca agtcgaacat tttcacggtc
     6781 aaggaatcac gacaaatgat gcattgaagc cactttctcg ttattgggat cggattgagc
     6841 gcccagaaca attgatgagc gcattaaccc gggcgtttga agttttgact gatcctgtga
     6901 ccgcgggtcc cgttacaatt tgcttacccc aagatgtcga aggggaggct tacgattacc
     6961 cagaggattt tttcaatgaa aaagttcatt attttgatcg ccgcctagct acagatcgtg
     7021 aattatcaga ggcggttaag ttaattgaaa gaagtctgaa accagtgatt atcgtgggtg
     7081 gcggggcgaa gtattcagaa gccggatcag ctcttaaacg tttttcggaa aagcataaga
     7141 ttcctttagt ggaaacccag gcaggaaaat caacggtggt tgctgatttt ccattgaatt
     7201 ttggtggtgt cggtgtgacc ggtggtgcac ctgccaatcg tcttgtgaaa gaagctgatc
     7261 tagtgattgg gttgggaaca cgctacaccg attttacgac agcctcaaaa acggcttttg
     7321 ataaggatca aacgactttt ttaaatatca atgtttcgcg gatgcaaagc tacaaaatga
     7381 acggtgtttc agtggtagct gatgccaaaa cggccctatt gcaaattgaa tctgagcttg
     7441 atgatttata tcaaacgcaa tatggtgacc tgttaactag tgttactagg gattggcaga
     7501 atgaacggga gcgtctggca aatttgaact accatgatgc tgattttgag atggagatca
     7561 aaaatcattt tggcgcagaa aagttagcca attatgcgca acaactaaca acagaattga
     7621 ctcaaaccgc ggctttaatc aaaatcaatc aaagtttgcc gctggatagt atcgtggttg
     7681 gggcggctgg ctctttgccc ggtgatatgc aacgtctgtg gaaagcgaag acagttaact
     7741 cttataatat ggagtatggc tattctacga tgggatatga ggtagcgggt gcttatggtg
     7801 ccaagattgc gcatccagat aaagaagttt ttgcttttgt tggagatggt agttttcata
     7861 tgttgcattc ggagctactt tccagtattc aatatgggaa aaaaatcaat gtcttacttt
     7921 ttgataactc aggctatgga tgtattaata atttacaaat gggcaatggg atgccatctt
     7981 ttggaacgga actgacagat tatcatgagg agatcatgac ggttgatttt gcaaaaatag
     8041 ccgaagctta tggggcggtt gcttataatg tcggtaatat gtctgaactt gagaaatctc
     8101 tagctgaaat agctgagtct gaccggagta cattaatcca tattaaagtt ttacctaaaa
     8161 ctatgacgga tggatacgat tcatggtgga ataccgggat tgctgaagtt actgcagatg
     8221 gttctactga aaaagtttta gctgaaaaag aagaaaaact agcaggggcc tttcaatact
     8281 gagaagccca gaaaggtgtc aaaatgtatc aactaacttt gccgcaaaaa atcatttacg
     8341 gcgatcaagc aatcaaggaa acaggaaaaa tcgctggtaa ccttggtttg aaaactttga
     8401 ttattacaga taaactaatc gtctctttga accttttgga aggtgtgctt gccagcctaa
     8461 aagaggaaaa tctgacctgg aaaatttttg atggcgttga ccgcgagcca acggaccaga
     8521 ttgtagctga agcattagct ttttaccaac aggaagatta tgattcgatt attgcggtgg
     8581 gcggtggcag ctgtatcgac actgctaaag ctgtggctgt gttaatcaat aatagtgggt
     8641 cctatgctga atttgctagt ggccaactga ttgcccaaaa tccactgcct ctaattgcaa
     8701 tacctacgac tgccgggact ggctccgaag tgacagatgt gaccgtaatc acagatacgc
     8761 aaaaagatat taaatatatg atgaaacagg ctgctttttt atcctatgca gcgattgttg
     8821 atccacaaat gagtcgtaca agtccagcat cagttacagc agcaaccgga ctagatgcat
     8881 tatgtcatgc tttagaaagt tatatttcta aaaaagccca gcctattaca aggctctttt
     8941 cactttcagc aataaagttg attatggaaa atttaccgat tgcctatagc gagccggaaa
     9001 acttaatagc ccgtgaaaac ttggcgcagg cagcaatgga agcgggaatt gccttttcaa
     9061 atgcttcggt taccttgatt catgggatgt caagacctgt tggcgctctg tatcatatcc
     9121 ctcatggcat ttccaatgcg attttattag aggcggtgat tgattacaca aaacctgtaa
     9181 tcatctcgga gttggctggt attgcgaaat atcttaatcc tgaaaaagaa ggtacggatc
     9241 aggagttggc agagtgcttc tttcaagaat tgcaggactt gattgagaaa ttagctgttc
     9301 ccaattacgc aagttttggt attgaaaagg cagcttttat tgcatctttg gataaaatgg
     9361 caaatgatgc aattgccagt ggtagcccca ataacaatcc tttggtacca acaattgagg
     9421 aaataaaaat gatttattta gctagttggc aagaatagtc aaagaaaagt aggtagaaaa
     9481 atggagataa aaacagatct taattcggaa gattctagtc agttggctga gtttctttta
     9541 atggtcttgg atcaacagca ggaacatttt ttccctgatt atcaaacaat cgataaatct
     9601 gtggccttgg ctgcttattc aggaactgaa ttggtaggcg gtctgctagg gaaaatcagc
     9661 gatcaaagct tgaacgtttc gttattagcc attgacccga aacatcaaaa ggatggtcta
     9721 ggaagtcagt tgatcgaaga agtcgaaaaa attgcggcaa aaaaccaagt cattaatctt
     9781 ctactttcga cgagaagtta tcaagcgctg gcgttttacc aaaaaaataa ttttgaaatc
     9841 tatggcgaac tcaaagacat gccgtttcgt ggcgttgata cgtattactt agtcaaacgt
     9901 ttgatggcat aacgtttaaa gttccaataa agaataaaga ggcacaaaaa tgacctacaa
     9961 agaaatacca gtctctgttc aacaatttat ggaaaaaatc acgactcttt gtggtaatga
    10021 ccacccacaa tgggcagcaa acattaatgc tgcctttgcc aatactcttt taacaacggt
    10081 taagcgacat gaagatggca caactttttt gttgactggc gatattcccg caatgtggtt
    10141 gcgggattca actgcccaag ttcggcctta tttagtcatc gccaaagata atccagattt
    10201 ggcggatatg attagtggtt tagtaaaaaa acaattcttc tatattaact tggatcctta
    10261 tgcaaatg
//
LOCUS       DACGLE010000090         9669 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0534
            SAMEA3304449-rid9560453.denovo.095, whole genome shotgun sequence.
ACCESSION   DACGLE010000090 DACGLE010000000
VERSION     DACGLE010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304449
            Sequence Read Archive: ERR985064
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9669)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9669)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702225.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702225.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 20:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,045
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 27 of 67
            Pseudo Genes (incomplete)         :: 25 of 67
            Pseudo Genes (internal stop)      :: 19 of 67
            Pseudo Genes (multiple problems)  :: 4 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9669
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304449-rid9560453.denovo.095"
                     /strain="VREN0534"
                     /db_xref="taxon:1352"
     gene            complement(14..706)
                     /locus_tag="IXT20_002434"
     CDS             complement(14..706)
                     /locus_tag="IXT20_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAQ4860857.1"
                     /translation="MQIELQHTHDKETFEKNYKVYVELARDSAMKYGIPLTLDTPYNQ
                     PGIKSHLWVTQNIWGDHTDPYGYLSEIGVSKEKLAYDLAHGFTDDNPTTSEDKPVIDP
                     TRAGAANSTLTDGTNYAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELAR
                     VRADGIYRPDVNQAYNTLGNVGYHVSFNMRDFPNKKVYVMMRATNDPEGNTKGGAQDF
                     HDKRWYLNIPQR"
     gene            791..967
                     /locus_tag="IXT20_002435"
                     /pseudo
     CDS             791..967
                     /locus_tag="IXT20_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348541.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase"
     gene            complement(1143..1712)
                     /locus_tag="IXT20_002436"
     CDS             complement(1143..1712)
                     /locus_tag="IXT20_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HAQ4860858.1"
                     /translation="MELKKLEVPTSSITEVKRSPMDVFAQARDAGTGVYIFNREKVAG
                     VMLTQEQYETLLQELDVLRQTVKVERKEPKEINEEPINKTASVVSNAEGMEELAQTLQ
                     HSLITGVTITAKNLDERMVALGFITKKTGFGGVVDMLSELMETGKINYQLRRKPQNRT
                     IIAEIIGEQNSQSQLMDKIIIKKIFLHEL"
     gene            complement(1786..2109)
                     /locus_tag="IXT20_002437"
     CDS             complement(1786..2109)
                     /locus_tag="IXT20_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4860859.1"
                     /translation="MLLLKTLQERGYLDVHPPEVKIVFFFRYEHQEARRLAGVLNKTL
                     RHRKRISIHVCTRNKPPRYADLVIIDHFFPLDHNEDQKTYLYHPSFGIERLLTDINYW
                     LGKNR"
     gene            complement(2263..3075)
                     /locus_tag="IXT20_002438"
     CDS             complement(2263..3075)
                     /locus_tag="IXT20_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008274430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4860860.1"
                     /translation="MRWQKLLSKNAQNLFSLINLMKDLIWRKADLAEELKIDVKTVDR
                     HLKRLAESDCPIQLILHKKTVQLVYDQGYSEPYLLFVFLIQSPPFCLLKNLLLNEPYE
                     QKYDRSTQERLRKWFGEYHLSFSYKKAQIFGSERKVRYLLFCFLKDFPHFFADDGEQS
                     FSEMFIQLLKQRKDPAEIEILGDSRTLFFELFPELLFREEQILPIKEKNYLAILRLSC
                     NQITEKRWKRIEETQDYRKLTIVTEKLDSLFWLNSEFESESKSKLTKVLYQE"
     gene            3429..4277
                     /locus_tag="IXT20_002439"
     CDS             3429..4277
                     /locus_tag="IXT20_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008274428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HAQ4860861.1"
                     /translation="MIVALLAGGHVLFEDIPGVGKTLLVKALAKAVQGTFSRVQCTPD
                     LLPSDILGFSVYNSQSKEFEFRPGPIFTTILLADEINRTTPRTQSALLEAMAENHATI
                     DNNTYPLDNHFFVLATQNPIEYEGTYPLPEAQLDRFLFRLQIGYPSFDDELLLLLDKF
                     EKVLDNLNEVLNSYEIEEIKTNVANVFVSPEVASYALQLVSATRSHAAIQLGISPRGS
                     LSFIQAAKAYALIHGRNYVTPKDLQDLVPYVFSHRLIFYDRSLKEDEKKQIIHTLVAQ
                     IPVPVR"
     gene            4291..5364
                     /locus_tag="IXT20_002440"
     CDS             4291..5364
                     /locus_tag="IXT20_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HAQ4860862.1"
                     /translation="MEKIKDYFINFVLFLVYFIIVLFALVFNNSLGWFLLAFFTLYVS
                     WSLISLSSFLKKLSVTCTLQPLVYRKKSMQVIFSIAKKRTFSLPLPRLQIGFPAIFAE
                     KKQEILILTNKPQEVQLLWQPKERGCHETLTVIFTAYDSLGLFRKSAKREISFPVTVL
                     PAFCKIQAEKLQRVLEKKQQLNAYQRGLDFREHRAYRPGDSFNRIDWKLSAHTQEWLY
                     REYDYQEEECSPLFCFWGSHSPKFEPLLDLYFSLWHLRKEKQPELLILGNRAFHGKDP
                     GHTYFASLEAGDEKAFFAHLKKYAKKQIVLFVPENSPEVSEAIETLSKDRTVYLVYFV
                     EKQLMVQEKDKICSLPGGEWIDD"
     gene            5354..7525
                     /locus_tag="IXT20_002441"
     CDS             5354..7525
                     /locus_tag="IXT20_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HAQ4860863.1"
                     /translation="MTIKWEKWLCGVYSFVILSVGAPIFVVANQLQNSYLLILTIGVL
                     CLIVCCIPKKWVRLLLSLPVMIGCLFLYFPSKTFSLLWILQFFSSVTEEFFHLSQGKL
                     YYLPEKTAFFLIMLLIYLFITLTVDYDYLYAPFLSLMAYFMMLTVFREKDLLYEMIAV
                     VTVLFVYLAARQFFSMRLLSGNTRFQSLTTVLLICFLTISAILPLYLPKVHQSLEETS
                     EPIREYLNDEGLYDTLHEYQLTGSARTGFSENDEQLGGPLYDNGEVVFTANQKGNGYW
                     KIENKNHYTGSGWIEDQDAPIQPIQEFPVDITSYQTVMEDKENITLTLKKQAFLPYPY
                     GNTTLTDFSLASGQTLYYLPESQRFIPVPNESTRSATLQTERLVFTREELKSSQLKAD
                     DLENQQTNLQLPKSLPKRIEDLAVELTKNEATLIRKVEAVEQYLKTSGNFRYSKTDTG
                     HTPKEHDYVDYFLFDSKVGYCDNFSTSMVVLLRTLGIPARWTKGFTNGLRTGTDNQGN
                     EEYSILNSHAHSWPEVYFPGFGWLPFEPTPSFANPMGSQPVAETTETVQPAIEQPNES
                     ENMAETISSERSEVIPSEETTLTPAYKIVLFSLGVCLYVLLFFLLLFRKSLRWTSYLI
                     YLHFFASTNTIAYQKLLNHLETLVPRKPAESLQEYSLRIEQEFKLDLRFIRLTTQYEA
                     EIYGRQADPVNFDKKTYLEITHIIRKTVYKKVLTKGSRFKK"
     gene            complement(7637..9658)
                     /locus_tag="IXT20_002442"
     CDS             complement(7637..9658)
                     /locus_tag="IXT20_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000069452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HAQ4860864.1"
                     /translation="MANSKQMDHSMHMDHDHGGMDHSMHMGNFKQKFWLSLILAIPII
                     LFSPMMGMEFPFQVTFPGSDWLVLILATILFIYGGQPFLSGAKMELKQKSPAMMTLIA
                     MGITVAYIYSVYSFIANLLNPHTHVMDFFWELATLIVIMLLGHWIEMNAVSNASNALQ
                     KLAELLPESVKRLTKDGKEETVSLKEVNEGDRLIVRSGDKMPTDGVILKGETIVDESA
                     VTGESRGIKKQANDKVIGGSINGDGTIEIEVTGTGENGYLAKVMEMVRKAQGEKSKLE
                     SLSDKVAKWLFYVALIVGILAFIAWLFLTDLPNALERMVTVFIIACPHALGLAIPLVV
                     ARSTSIAAKNGLLLKNRNALEQANDLDVIMLDKTGTLTEGKFTVTGVEVLDDAFNKNE
                     ILQYLGALEANANHPLAVGIMNYLKEHEIKPYQAENLKNLSGVGLEATVKNQQVKIVN
                     EKEVERLGLNVEQALLKPYQEQGNTISFLILENHLAAIVALGDVVKTEAKEFIRTLKE
                     RKITPVMLTGDNKNAAQAVADYLGIEEYYGGLLPDDKEAVVQKYLDQGKKVIMVGDGI
                     NDAPSLARASIGMAIGAGTDIAIDSADVVLTNSDPKDILHFLDLAKQTRKKMIQNLWW
                     GAGYNILAIPLAAGILAPIGIILSPAVGAVLMSLSTVVVALNALTLKIK"
BASE COUNT         2819 a         1954 c         1742 g         3154 t
ORIGIN      
        1 agggggggat tttttatcgt tgtggaatat ttaaatacca acgcttgtca tgaaaatctt
       61 gtgctccacc cttagtattt ccttctggat cgtttgtcgc acgcatcatg acgtatactt
      121 tcttattagg gaaatcacgc atattgaaag atacatgata gccaacattt cctaaagtat
      181 tataagcttg atttacgtct ggtctataaa tcccatcagc tcttactcta gctagttctt
      241 ttccagtatt gtagtccata atgaaaatat actcgtattt ataattagca atgtgccatc
      301 cagctacatg caagtttgcg ttttcgattt ctccgaactg atcaatgtgg gcgtaatttg
      361 ttccatctgt cagcgtagaa tttgcagcac ctgctcgagt tggatcaatt actggtttat
      421 cctccgaagt agttggatta tcatccgtaa aaccatgagc caaatcatag gctagttttt
      481 ctttacttac acctatttca gaaagataac cgtaaggatc tgtatgatcg ccccaaatat
      541 tttgtgttac ccataaatgc gatttgattc ctggttggtt ataaggagtg tctaatgtta
      601 atggaatacc atatttcatt gctgaatctc ttgccaattc aacgtatact ttatagttct
      661 tttcaaaagt ttctttatca tgtgtgtgtt gtaactcgat ttgcacagga cttttggctc
      721 acgaggttgt cctactctac atcttgctga tagatatgct gaaaggaaaa caccaacatt
      781 atgttttgct ttttcagcta gaacgagaca agcaggaata cctttagaag atatcaaagg
      841 ttttttaggt cataaagatg tttccactac acaagtctat gcccatattt ctcccgaagt
      901 gaaaaaaaga tcaatgaatc aactagaaaa ctatatagaa gagcaaataa aaaagcactc
      961 aaattgagtg cttttttaag ctcactatca ctaaaactat cactaatttt atttataagc
     1021 gaacatgttt ctatcactga ttttttaaat ctagcgaaaa acccttatct taaaggcgtt
     1081 cgttcaattc tttggcaagg tcttcaaagc ctggcttgcc tagaagtgcg aacatggttt
     1141 tcttatagct catgtagaaa aattttcttg atgatgattt tatccatcaa ttgagattga
     1201 ctgttttgct ctcctatgat ttcagctatg attgtccgat tttgcggttt tcttcttaac
     1261 tggtagttga ttttcccagt ttccatcaac tcgctaagca tatccacgac tccgccaaat
     1321 ccggtctttt tcgtaataaa acctagtgct accattcgtt catctagatt ttttgctgta
     1381 atcgtcacac cagtaatgag cgagtgctgt aatgtttggg ccagctcttc catcccttca
     1441 gcattggata cgacggaagc tgttttgtta ataggttctt cgtttatttc ttttggttct
     1501 ttcctctcca ctttcactgt ttgacgaagg acatctagtt cttgcaagag cgtttcgtat
     1561 tgttcttggg tcagcatcac gccagctact ttttcacgat taaatatata tacgccagtt
     1621 cccgcgtctc gcgcttgagc aaatacatcc atcggcgatc gtttcacttc tgtgatcgaa
     1681 gatgtgggaa cttctaattt ctttaattcc aatggatttc ctcctttact aatcgctttt
     1741 tattatagca cagactgact tgttttgatc ttttttccaa aggttttatc ggtttttgcc
     1801 taaccagtaa ttgatatcgg taagaagtct ttcgatacca aatgaaggat ggtacagata
     1861 cgttttttga tcttcgttat ggtctagtgg aaaaaaatga tcgatgatca ctagatctgc
     1921 ataccgaggc ggcttatttc gagtacatac atgtatagag atccgttttc gatgtcttag
     1981 cgttttgttc agtaccccag caagtcttct tgcttcttgg tgttcatagc gaaaaaaaaa
     2041 gacgattttc acttcaggag ggtggacatc taaataccct cgttcctgga gtgttttcag
     2101 taaaagcatc ccccactcta ctgcggggtg tgcaagtgct tgttggatcg ctagccgaaa
     2161 ttgatatagt ttatctttgg aattgggaaa tttttctaat gtttcctgat aataacgaac
     2221 ttcttctatc agccggtcat ggataccaat tacgtaacga agtcattctt ggtaaagtac
     2281 ttttgtcagc tttgatttgc tttcgctctc aaattcgcta ttcagccaaa acagcgaatc
     2341 aagcttttca gtcacaatcg tcaatttccg atagtcttgt gtctcttcta tccgtttcca
     2401 ccgcttttca gtaatctggt tacagctcaa gcgcaaaatt gctagataat ttttttcttt
     2461 tattggcaaa atctgctcct ctctaaatag caattcagga aacaattcaa agaatagtgt
     2521 tcgactgtct cctaaaatct cgatttctgc cggatccttt ctttgcttca ataattggat
     2581 gaacatctca gaaaaggact gctctccatc atctgcaaaa aaatgcggaa aatctttcaa
     2641 aaagcaaaac agcagatatc ttacttttct ttctgaacca aagatctgtg cttttttata
     2701 agaaaaagaa aggtggtact cgccaaacca ctttctcaat cgctcttgcg tagaacgatc
     2761 gtacttctgc tcgtaaggtt cattcaatag cagatttttc agtaaacaaa acgggggtga
     2821 ttggatcaga aaaacaaaca agagataagg ttcgctatat ccttgatcat aaactaactg
     2881 aacggttttc ttatgaagaa tcagctgaat gggacagtcg ctttccgcca aacgtttcag
     2941 atgccgatcc actgttttaa catcgatttt cagttcttca gctaaatcag cttttcgcca
     3001 gatcaaatct ttcattagat tgattagtga aaataaattc tgtgcatttt ttgataatag
     3061 tttttgccat ctcatcgatc atttctcacc tgatttctaa acatattgtt tgatcaaaat
     3121 gggttcactc ctaaaaagat aaaaaactcc ctttctattt atttaaatac gcaaatcttc
     3181 ctcattattt tttattttac tcaatttaat cgtattctca tttcaaaacg aatgtacttt
     3241 aaaaaaacat aataagatca ctttgcttgt aggatgtatt cccttccatt acaatgaaat
     3301 aaatagcgat tttatgagaa ggtgatgtga aaatggaaga aaaaatagaa gaatccaaac
     3361 aaaaaatcga tcagttgata taagaattag aaaaagcggt catcggcaaa cgttcaacta
     3421 tccaattgat gatcgtggcc ctattagcag gtggtcatgt cttattcgaa gatattcccg
     3481 gtgtaggtaa aacgttactg gtcaaagcac tagcaaaagc tgtacaggga accttcagca
     3541 gagtccaatg tactcctgat ctgctgccta gtgatattct tggtttttcc gtatacaatt
     3601 cacaatcaaa agagtttgaa tttcgtccgg gaccgatctt taccacgatc ttgttagcag
     3661 atgagatcaa tcgtacgact cctcgtactc agtcagcact tttagaagca atggccgaga
     3721 atcatgcaac gatcgacaac aatacatacc ctctagacaa tcattttttt gtcttagcta
     3781 cgcaaaatcc aatcgagtac gaaggaacat atcctttgcc ggaagcacag ctcgatcgtt
     3841 ttttattccg actacaaatc ggttatcctt cttttgatga cgaacttttg ctacttttgg
     3901 ataaattcga aaaagtgttg gataacttaa atgaagtatt gaacagttat gaaatagaag
     3961 aaataaaaac aaatgtggcg aatgtctttg tcagtcccga agtggcatct tatgccttgc
     4021 agttagtgtc agcaacaaga agtcatgctg ccatccagct tggcatcagt ccacgaggaa
     4081 gcctttcctt tattcaggca gcaaaagctt atgctttaat ccatgggaga aattacgtca
     4141 cgcccaaaga tttgcaggat ttagttcctt acgtgttttc ccatcgcctg attttttacg
     4201 atcgttcatt gaaggaagac gaaaaaaaac aaatcattca tactcttgtt gcacaaattc
     4261 ctgttcctgt gaggtagatt gaggtagata atggaaaaaa tcaaagatta tttcattaat
     4321 tttgtgttat ttctggtata tttcatcatt gtgctttttg ctcttgtatt caacaattct
     4381 ttaggctggt tcttgttggc cttttttact ctttatgtat catggagctt gatttcatta
     4441 agcagctttc taaagaaact ttcggttact tgtacattgc agccactcgt ttatcgcaaa
     4501 aaaagtatgc aggttatttt ttccatagca aaaaagagaa ctttttcttt acctctccct
     4561 cgtttacaaa tcggcttccc agcaatcttt gcagaaaaaa aacaagagat tttgattctt
     4621 acaaataaac cgcaagaagt ccaattgctt tggcagccaa aagaaagagg atgccatgaa
     4681 acattaactg tcatttttac agcctatgat agtctaggac ttttcagaaa aagtgcaaaa
     4741 agagagatct cttttcctgt gactgttctg cctgcttttt gtaagattca agcagaaaaa
     4801 ttacagagag ttctggaaaa aaaacagcag ctgaacgcgt accaaagagg tcttgatttt
     4861 cgggaacatc gcgcttatcg tccaggtgat tctttcaacc gaatcgattg gaaattgtct
     4921 gctcatactc aagagtggtt ataccgtgaa tacgattatc aagaagaaga atgttctcct
     4981 cttttttgct tttggggttc gcacagtcca aaatttgaac ctctgctaga cttgtatttt
     5041 tctttatggc atttgagaaa agaaaagcag ccggaacttc tgatattagg caaccgcgcc
     5101 tttcatggca aagaccccgg tcatacctat tttgcttctt tggaagctgg cgatgagaaa
     5161 gcttttttcg cacacctaaa aaaatatgca aaaaaacaaa tcgtcctctt tgttcctgag
     5221 aattctcctg aagtctcaga agctattgaa acattgtcaa aagatcgaac ggtttatctt
     5281 gtttatttcg tagaaaaaca actcatggtc caagaaaaag acaagatatg ttcattgcca
     5341 ggaggtgaat ggattgacga ttaaatggga aaaatggctg tgtggcgttt actcctttgt
     5401 gatactaagt gtaggtgctc cgatatttgt agtagctaat caattgcaaa acagctacct
     5461 tcttatccta actattggtg tcctatgcct tattgtctgc tgtattccta aaaaatgggt
     5521 acgtttgctg ctttctttgc cagtaatgat aggttgtttg tttttgtatt ttccttcgaa
     5581 gacattttcc ttattatgga tcttacagtt tttttcgtca gtcacagagg aattcttcca
     5641 tctatcccaa ggtaaacttt attatttgcc agaaaagaca gctttttttc tcataatgtt
     5701 gctgatctat ttatttatta cattgaccgt ggattacgac tatttgtacg caccctttct
     5761 ttctttgatg gcttacttca tgatgcttac cgtttttcgg gaaaaagatt tactgtacga
     5821 aatgattgcg gtagtaactg ttttgttcgt gtatctggca gcaaggcaat ttttctccat
     5881 gcggttgttg tcgggaaaca ctcgattcca gtcattgact acagttctgc ttatttgttt
     5941 cttaactatt agcgctatct tgcctttata tcttccgaaa gtacaccaat ctctcgagga
     6001 aacgagtgaa ccgatccgag aatatcttaa tgatgaaggg ctttatgata cactccatga
     6061 gtatcaatta accggaagtg caagaacagg cttcagtgag aatgatgaac agcttggtgg
     6121 acctttatat gataatggcg aagttgtttt tacagcgaac caaaaaggaa atggctattg
     6181 gaagatcgaa aataaaaatc attacaccgg ttctggatgg atagaagatc aggacgcacc
     6241 catccaacca atccaagaat tccctgttga catcacgagc taccaaaccg ttatggaaga
     6301 caaagaaaac attacattga ccctaaaaaa acaagctttc ttaccttatc cttatggaaa
     6361 tactaccttg actgattttt cattagctag tggacaaacg ctttactatt tgcctgaaag
     6421 ccagcgattc attcctgtcc cgaatgaatc gacacgttct gcaactttac aaacagaaag
     6481 gttagttttt acgagagaag aattaaaaag cagtcagtta aaagctgatg atttagaaaa
     6541 tcagcagaca aatcttcaat tgccaaaaag tttgcctaaa cgtattgagg acttggcggt
     6601 cgaattgacc aaaaatgaag caactttaat tcgtaaagtc gaagctgtcg aacagtattt
     6661 aaaaacaagc ggaaatttcc gctattccaa aacggatacc ggacatactc cgaaagaaca
     6721 cgattatgtg gattatttcc tctttgattc aaaagtcggc tattgtgaca acttttcaac
     6781 ttctatggtc gtccttcttc gaacacttgg tatcccagct cgctggacaa aaggttttac
     6841 aaatgggctc agaacaggca cagataatca agggaatgag gaatacagca ttctcaatag
     6901 tcatgctcat tcttggccag aggtatactt tcctggattt ggctggcttc catttgaacc
     6961 aaccccttca tttgccaatc caatgggttc acaaccagtt gctgaaacaa ccgaaaccgt
     7021 ccagcctgct atcgaacaac caaatgaaag cgaaaatatg gcagaaacta tttccagtga
     7081 gagatctgag gtcattccat cagaagaaac aacactcaca ccagcttata aaatcgtctt
     7141 attcagcctt ggggtgtgtt tatacgtctt gttgttcttt ctattgttat tcagaaaatc
     7201 tctgcgctgg acaagttatc ttatctatct tcattttttt gcttctacta atacgattgc
     7261 atatcaaaaa cttcttaacc atttggaaac attggtaccg cgcaaacccg cagaatcttt
     7321 gcaggaatat agcctgcgaa tagaacaaga attcaagcta gatctgcgtt tcattcgatt
     7381 aactactcaa tatgaagcag aaatatatgg acgacaagca gatccggtaa atttcgataa
     7441 aaaaacctat ttagaaatta cgcatataat aagaaaaacg gtatacaaaa aagttctgac
     7501 aaaagggagc cgcttcaaaa aataagataa aatttctgga gaatctactt ctggaatgcc
     7561 gagaaccagc tgcttgagta tcgattattc gaaaaaaagg ttgtgacacg ttttttgtca
     7621 caaccttttt tcattattat ttgattttca atgtcaaagc atttagagct acaactaccg
     7681 tacttaacga catcaagaca gcaccaactg ctggacttaa tatgatacca attggcgcaa
     7741 gaattccggc tgctagcgga attgcaagaa tattgtaacc tgccccccac cagaggttct
     7801 ggatcatttt ctttcgcgtt tgcttggcta gatcaagaaa atgcaaaata tcttttggat
     7861 cgctgtttgt caatacgaca tccgcagaat cgattgcgat atctgtccct gcaccgattg
     7921 ccatcccgat tgaagcacgg gctaaacttg gcgcatcatt gatcccatca cccaccatga
     7981 ttactttttt cccttggtca aggtatttct ggacaacagc ttctttatca tctggtaata
     8041 aaccgccata atattcttcg atccctaaat aatctgctac tgcttgggca gcgtttttat
     8101 tgtcaccagt aagcatgaca ggagtgattt ttctttcttt caatgttcgg ataaattctt
     8161 ttgcttctgt ttttactacg tcacctaaag caacgattgc agctagatga ttttctaaaa
     8221 tcaggaagct gatggtattt ccttgttctt ggtaaggttt caataatgct tgttctacgt
     8281 ttaatccgag acgctcaact tctttttcat taactatttt cacttgttgg tttttgactg
     8341 ttgcctctag cccaactcct gaaagatttt ttagattttc ggcttgataa ggcttgattt
     8401 catgttcttt caaataattc ataataccaa cagccaatgg atgattggca ttcgcttcca
     8461 atgctcctaa atattgaagg atttcatttt tattgaacgc atcatcaagt acttcgacac
     8521 ccgttacagt aaattttcct tctgttagtg ttcctgtttt gtccagcatg atcacatcta
     8581 gatcattggc ttgctccagt gcattgcggt ttttcaaaag taatccgttt ttagctgcta
     8641 ttgatgtaga gcgagcaaca actaatggga tagctaaacc tagagcatga ggacaggcga
     8701 tgataaagac tgttaccata cgttctaatg cattaggaag atctgtcaag aacaaccagg
     8761 cgatgaatgc caggataccc acaattaatg cgacataaaa cagccatttt gccactttgt
     8821 cagataatga ctctagcttg gatttttctc cttgtgcttt tcgcaccatt tccataacct
     8881 tcgctagata accattttca ccagttccag ttacttcgat ttcgatcgtt ccatccccat
     8941 tgatagaacc gccaatcact ttgtcattgg cttgtttttt gattcctctt gattcgccag
     9001 tcacagctga ttcgtcaaca attgtttcac ctttcaagat tacaccatct gttggcattt
     9061 tatcaccaga ccggacgatc aaacgatcac cttcgtttac ttctttcaat gaaactgtct
     9121 cttcttttcc atctttcgtc aagcgtttta ctgattctgg cagtaattct gctagttttt
     9181 gtaaagcatt gctggcattc gacacagcat tcatttcaat ccagtgacct agtagcataa
     9241 taacgatcaa agtagccaat tcccagaaga aatccatgac gtgtgtatgt ggattgagca
     9301 gatttgcgat aaacgaatag acactataaa tatatgcaac tgtaatcccc atcgcgatca
     9361 gtgtcatcat agcaggactt ttttgtttca attccatctt agctccactt aaaaatggct
     9421 gaccgccata gataaatagt atagttgcta agatcaatac gagccaatca gaaccaggaa
     9481 acgttacttg aaatggaaat tccattccca tcattggtga aaaaagaatg attgggatag
     9541 ctaggataag cgatagccaa aatttttgtt tgaaatttcc catgtgcatg gaatgatcca
     9601 tgccgccatg atcgtgatcc atgtgcatcg aatggtccat ttgtttgctg tttgctatat
     9661 ctccatggt
//
LOCUS       DACGLE010000091         9524 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0534
            SAMEA3304449-rid9560453.denovo.096, whole genome shotgun sequence.
ACCESSION   DACGLE010000091 DACGLE010000000
VERSION     DACGLE010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304449
            Sequence Read Archive: ERR985064
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9524)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9524)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702225.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702225.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 20:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,045
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 27 of 67
            Pseudo Genes (incomplete)         :: 25 of 67
            Pseudo Genes (internal stop)      :: 19 of 67
            Pseudo Genes (multiple problems)  :: 4 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9524
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304449-rid9560453.denovo.096"
                     /strain="VREN0534"
                     /db_xref="taxon:1352"
     gene            complement(412..1452)
                     /locus_tag="IXT20_002443"
     CDS             complement(412..1452)
                     /locus_tag="IXT20_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HAQ4860865.1"
                     /translation="MNDFNYYKSKEIYREKYYQMPKVFFTNEKYMDLSNDAKIAYMLL
                     KDRFDYSVKNNWVDSDDNIFFIFTVEELMKLLQCREGKVSKIKKELEKAGLLKQKRGR
                     VNKRDGKIESMPNLLYLGKPEVTNQDVFKIMEEEDNTDTTVIAKIANTAKPREINDTT
                     VIAKIANTAKSSHIKDSTVIAKIADNLFYSNSIDTNRHIIDTETDQLQNQVLLDNFVD
                     IMKEDSINTFVPENVLNLIKTFSSTYEDAQKTVQTIHNAKKKAEELEQTVVVYEELES
                     YGIDADKGLYMTLLKAYQKQKTEKVNNLQNLIFVYVKNWFVEKAIAAKLANEQRETFN
                     ELPTVSTENWLE"
     gene            complement(1800..2126)
                     /locus_tag="IXT20_002444"
     CDS             complement(1800..2126)
                     /locus_tag="IXT20_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4860866.1"
                     /translation="MAEDNRSQAAVSRLLGNKNKKNFKSKEVEEIELTKKNISKVIKD
                     QGSSVRTADSIRINPILMSALKYWTTIAESDKSKPDVIEDALLKSIPEEYLIEGYKLA
                     KKQNKI"
     gene            complement(2113..2901)
                     /locus_tag="IXT20_002445"
     CDS             complement(2113..2901)
                     /locus_tag="IXT20_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HAQ4860867.1"
                     /translation="MARILSVANQKGGVSKTTTSVNLAACLKFVYGKNVLLVDIDPQG
                     NATDNVGFDIDGTNQPTIYEVLKGEADITDAILDYKGIDVLPADIALSSAEREFTQVG
                     SEHRLKRVLQPIKDNYDYIIIDCPPSLGMLTVNAFTVSDEIIIPVEAAYFSLKGLIKL
                     SETIETVKEYTNDKLEVRGVLFTKYNKRYNISKEMTSAATEISRVIGAKIFKTYIRRT
                     ITVDEAQAAGSDLINFNKPSTAEDDYKAFTEEYLKEVGDFDGRR"
     gene            complement(3269..3559)
                     /locus_tag="IXT20_002446"
     CDS             complement(3269..3559)
                     /locus_tag="IXT20_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000026576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgN"
                     /protein_id="HAQ4860868.1"
                     /translation="MSINTFVYSHPINVYIIKNLGITVEQFCELYAYPQGTVASWITR
                     QRRIKSLPASFVYDLSLASSLNMSDVYEKLLSLEKEYDSFKIKHDKKIKKHI"
     gene            complement(3695..3874)
                     /locus_tag="IXT20_002447"
     CDS             complement(3695..3874)
                     /locus_tag="IXT20_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001021554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4860869.1"
                     /translation="MNIIQQYELKYITFDQLSEEIWGYGQRLINEVGVERFSFYVEAA
                     AGYHNFRFYIFPLYI"
     gene            complement(3871..4221)
                     /locus_tag="IXT20_002448"
     CDS             complement(3871..4221)
                     /locus_tag="IXT20_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4860870.1"
                     /translation="MIDYNDRGRLKWAGFYLSDHTEEIDADNEQRTSQNLAKEQMTTE
                     EIAEVLNAAVIKNRSVSIQKEERNAEGYYPEDIIGKILGSDELGIYVGKDKIHYDEIR
                     HIEFYQDNKWSRLE"
     gene            complement(4214..5539)
                     /locus_tag="IXT20_002449"
     CDS             complement(4214..5539)
                     /locus_tag="IXT20_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HAQ4860871.1"
                     /translation="MMDYSKEPVNDYFLIDMKSFYASVECIERNLDPLTTELVVMSRA
                     DNTGSGLILASSPEAKKRYGITNVSRPRDLPQPFPKTLHVVPPRMNLYIKRNMQVNNI
                     FRRYVADEDLLIYSIDESILKVTRSLNLFTTEGTRSQRRKKLAQMIQENIKEELGLIA
                     TVGVGDNPLLAKLALDNEAKHNEGFVAEWTYENVPEKVWNIPEMTDFWGIGSRMKKRL
                     NQMGIMSIRDLANWNPYTIKNRLGVIGLQLYFHANGIDRTDIAIPPEPTKEKSYGNSQ
                     VLPRDYTRRNEIELVVKEMAEQVAIRIRQHNCKTGCVHLHIGTSILETRPGFSHQMKI
                     PITDNTKELQNYCLFLFDKYYEGQEVRHVGITYSKLVYTDSLQLDLFSDPQKQIDEEN
                     LDKIIDKIRQKYGFTSIVHASSMLEGARSITRSTLVGGHAGGNGGIKND"
     gene            5989..6603
                     /locus_tag="IXT20_002450"
     CDS             5989..6603
                     /locus_tag="IXT20_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001261742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAQ4860872.1"
                     /translation="MRVAYIRVSSIDQNEQRQIEEMKKFGAERIFIEKQSGATITHRP
                     VFQEALDYVRDQDIFIVEAIDRLGRNYDEIIASVNYLKKKNVKLIITSLPIMAEAIGN
                     PLLDKFIKDLIIQILAMIAEQERTESKRRQAQGIKIAKANGVYKGRPKLYSADAKDPQ
                     RRLVYKSIVEDLKNGVAIAKIAKDYNVTRQTVYRIKKDSMVNDK"
     gene            complement(6768..7772)
                     /locus_tag="IXT20_002451"
     CDS             complement(6768..7772)
                     /locus_tag="IXT20_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4860873.1"
                     /translation="MEYKFNLNLKEVLPTQGTININGQDNDDDIVIKKIEFIDSDINV
                     FFYSKEKLADFSNIAEMIERLKDFMISVWDVAMLADESNYTSSIYRLEDSNWIEISLE
                     LKTLPNSVNYIIRDITSEAQQLVNYMHGHESIPFSLILLKRSKNISNTRDRFISIITA
                     CEIGVKEFYQQEKPDLTLIFENLQSPPVDKLLGSMFKGYFNIEFPKEIRKEIKNFIEK
                     RNKLVHSPQGSKPTIEECFECYMAVLKTFNFFYKINDSFMYRDFYEESIELTPTEPNK
                     SQITLSKELERKVESQELAIGISITNVSTYKELYEAKSQDNKDFVENIDKEPTNTVGD
                     "
     gene            complement(8231..8488)
                     /locus_tag="IXT20_002452"
     CDS             complement(8231..8488)
                     /locus_tag="IXT20_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HAQ4860874.1"
                     /translation="MIKAWSDDAWDDYLYWHEQGNKSNIKKINKLIKDIDRSPFAGLG
                     KPEPLKHDLSGKWSRRITDEHRLIYRVENETIFIYSAKDHY"
     gene            complement(8481..8750)
                     /locus_tag="IXT20_002453"
     CDS             complement(8481..8750)
                     /locus_tag="IXT20_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HAQ4860875.1"
                     /translation="MEAVAYSNFRQNLRSYMKQVNEDAETLIVTSKDVEDTVVVLSKR
                     DYDSMQETLRTLSNNYVMEKIRRGDEQFSKGAFKTHDLIEVESDD"
     gene            complement(8881..9060)
                     /locus_tag="IXT20_002454"
     CDS             complement(8881..9060)
                     /locus_tag="IXT20_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4860876.1"
                     /translation="MPIAIGNKRLPVTLDEKRQKELQQLKQKYGKSESRIMCIALDLL
                     IAQEKAGFEVPALKK"
BASE COUNT         2941 a         1711 c         1352 g         3520 t
ORIGIN      
        1 tccaaaagtg cagaacgaaa atcaattcat cacaagcata tcagacaatt aaatttaaaa
       61 caactaaaaa tatacaaaaa agtgtaattg aatattagca gcactgcctg aaaagaagta
      121 atatcaggct tttcagcttg gggaaataag tgaattacct ctcttcatga attattataa
      181 ctcaaaaaag cacatatgca aacactagtt ccctattttt ttgaatgaaa aataactaaa
      241 atatctaacg attatgactt taaaacctac gtttgtgatt gttttcataa acttttaatc
      301 taaaaactcc ggaataatag ctatttattt gtaacatttg taataaaatt aaaatcataa
      361 aaaagttcta atttaaaatt gatcattttt atagtgatcg ctttttatca actactctag
      421 ccaattttcc gtactaactg ttggtaattc gttaaatgtt tctctttgtt cgtttgcaag
      481 ctttgccgca atagcttttt cgacaaacca attttttaca taaacgaaaa ttagattctg
      541 caaattgttt actttttcag ttttttgttt ttgataagct ttcagcaaag tcatatataa
      601 tcctttgtca gcatcaattc cataagattc cagttcttca taaacaacaa ctgtttgctc
      661 taattcttca gcttttttct ttgcattatg aatagtttgg acggtttttt gagcgtcttc
      721 gtaagtacta gaaaatgttt ttatcaagtt taatacattt tctggtacaa atgtatttat
      781 actgtcctct ttcatgatgt cgacgaagtt atctagtaaa acttgatttt gtaactgatc
      841 agtttcagtg tctattatgt gtctattagt gtctatagaa ttagaataga atagattgtc
      901 tgcgattttc gcaattacgg ttgagtcctt gatatgactg gattttgccg tatttgcgat
      961 tttcgcaatt acggtggtat cattgatttc tcgcggtttt gccgtatttg cgattttcgc
     1021 aattacggtg gtatcagtat tgtcttcctc ttccatgatt ttaaagacgt cttgattcgt
     1081 tacttctggc ttgcctaagt acaataaatt aggcatggat tcaatcttac cgtcacgttt
     1141 atttactctt cctctcttct gtttcagaag acctgctttt tctaattcct ttttgatttt
     1201 agatacttta ccttcgcggc attgtaacaa tttcattaat tcttcaactg tgaatatgaa
     1261 aaatatatta tcatcagaat ctacccagtt atttttgact gaataatcaa atcgatcttt
     1321 aagtagcata taagcaattt ttgcatcatt tgatagatcc atgtattttt cgttagtaaa
     1381 aaatacctta ggcatttgat aatatttttc acgataaatt tcttttgatt tgtaataatt
     1441 aaaatcattc atagccaatc ctctttctgt aggatagcgt tttgttcaaa actatgttac
     1501 aatgaattaa acaaatatat ctatccacta tatatttttc tactgctgat aagtcgccaa
     1561 actttgttaa agcagtagtc tttttttatc tttttttagt ttaccttaaa aaataaatta
     1621 aagcaaaccg ctctatttca aggtttaagc tcatagtaac tatatcacga tattacaata
     1681 atattatatc acgatatcgt aatattacaa tatgaagata agcgacttat cagctttgat
     1741 actactgatt tttataaaaa aatatcacga tattatgata tcgtgatatt gaatgtaaat
     1801 taaattttat tttgtttctt tgctagttta tatccttcaa tcaagtattc ttcaggtatg
     1861 gactttaata gcgcatcttc tataacgtct ggtttacttt tgtctgattc agcaatagtc
     1921 gtccaatatt tgagtgctga cattagaatt ggatttatac gaatgctatc tgctgttctt
     1981 acactacttc cttgatcttt tatgacttta cttatatttt tcttggttaa ttcgatttct
     2041 tctacttctt ttgatttaaa attcttttta tttttatttc ctagtaatct acttactgca
     2101 gcttgactgc gattatcttc tgccatcaaa atcaccaact tcttttaaat attcttctgt
     2161 aaatgcttta tagtcatctt cagctgttga gggtttatta aaattgatta gatctgatcc
     2221 agcagcttgc gcttcgtcaa cggttattgt cctacgaata taagttttga aaatttttgc
     2281 accaatgact ctagaaattt cggttgctgc agacgtcatt tctttagaaa tgttataacg
     2341 cttattatac ttagtaaata gaactcctcg tacttcaagc ttgtcgttcg tatattcttt
     2401 taccgtttca atagtttcac ttaactttat tagtcctttt aatgagaaat acgcagcttc
     2461 aactggaatg ataatttcat cagaaacagt aaatgcgttg actgtaagca ttcctaaact
     2521 tggtggacaa tcaattataa tataatcata gttgtccttg attggttgaa gtactctttt
     2581 aagcctgtgt tcacttccta cttgagtaaa ttccctctct gcagaagaaa gggctatgtc
     2641 ggctggcaaa acatcaattc ctttatagtc cagaatagca tctgtaatgt cagcctcccc
     2701 ttttaatact tcataaattg taggttggtt tgttccatct atatcgaaac ctacattatc
     2761 tgtagcattt ccctgaggat caatgtccac taaaagtaca tttttaccgt aaacaaattt
     2821 caaacatgct gctaaattaa cgctagtcgt agttttagaa actccacctt tttgattggc
     2881 tactgacaat attcttgcca ttaaaatatc ccccattcat cttatattat aatataatta
     2941 tatcataata ttgtgatatt gcaatatcgt atatgcatta tattgtaata ttgtgtgatt
     3001 attttgtttt aaagatatat atcggttcgg atataaagat tattcatatg ttattcgaaa
     3061 ttaaaataat cactttttat tactttaaaa ttttttatta atgataagaa atatgttttg
     3121 cgagcagcaa ctagaataat ttaaacatta ctaaactgtc tatattgtta ctaataattc
     3181 ccttcttata gtgcattttc ttagaaaaaa acaatattac gatatcatga tattaagata
     3241 tcgtaatatt atacaattgt gattgatatt atatatgttt ttttattttt ttatcatgtt
     3301 taatcttgaa ggaatcatat tctttttcta aagacaggag cttttcataa acatcagaca
     3361 tgtttaaact agaggccagg cttaaatcat aaacgaatga agcaggtaat gattttattc
     3421 tacgttgtcg ggttatccag cttgcaaccg ttccttgtgg gtaggcatat aactcacaaa
     3481 attgttctac agtgattcct aaattcttga taatgtaaac attgatcggg tgcgagtaaa
     3541 caaatgtatt gatagacatg gtgcgtaagc ctactttctt ttattattgc gtttgcaata
     3601 atagtttaac acacgcatta ttctagtatc tactcctatt tgtaaaaaca aaaaaggatt
     3661 catagagata actctataga atccgtaaaa taccttatat atataacggg aagatataaa
     3721 acctaaaatt atgataacct gctgcagctt ctacatagaa agagaagcgc tcaacaccaa
     3781 cctcatttat caaacgttgg ccataacccc aaatctcttc tgatagctga tcaaaggtta
     3841 tatactttag ctcatattgc tggataatgt tcattctaat cgactccatt tattatcctg
     3901 gtaaaattcg atatgacgta tttcgtcgta gtgaattttg tcttttccaa cgtagatccc
     3961 cagctcatca ctacctaaga tcttgccaat gatatcttct ggatagtatc cttcagcgtt
     4021 tcgttcttct ttttgaatag atactgatct attttttatg actgcagcgt tcaatacttc
     4081 cgcaatctct tcagtcgtca tttgttcttt tgctaaattt tgactggttc gttgttcatt
     4141 gtcggcatca atttcttcag tatgatcaga taaataaaac cctgcccact ttaaacgtcc
     4201 acgatcattg taatcaatca tttttaattc ccccatttcc gcccgcatga ccaccaacaa
     4261 gtgtgcttct agtaatgctt ctagcgcctt ctaacattga agaagcatgt acaatcgacg
     4321 taaaaccata tttttgccga atcttgtcaa taattttatc taaattttct tcgtcaattt
     4381 gcttttgtgg atcactaaat aaatccagct gaagcgaatc ggtgtacaca agcttgctat
     4441 aggttattcc aacatgcctt acttcttgtc cttcataata cttatcaaac aagaaaagac
     4501 aataattctg caattccttt gtattatctg tgatagggat cttcatctgg tgtgaaaacc
     4561 ctggtctcgt ttctaagatc gacgtgccaa tgtgtaaatg gacacaacct gttttacagt
     4621 tatgctgcct aatgcgtatc gctacttgtt ccgccatttc ttttaccacc agctcgattt
     4681 catttcttct agtataatct cgtggtaaca cttgtgaatt tccatatgat ttttcctttg
     4741 tcggttctgg tgggattgct atatctgttc gatcgatccc attagcatgg aagtacagct
     4801 gtaagccaat cacacctagt ctattcttaa tcgtataagg attccagtta gcaagatctc
     4861 taatgctcat aatccccatc tgattgagtc tttttttcat tcgagagcca attccccaaa
     4921 agtccgtcat ttcaggaata ttccatactt tttcaggaac gttttcgtac gtccattccg
     4981 caacaaatcc ttcattatgc tttgcttcgt tgtctaaggc tagtttagcc aaaaggggat
     5041 tatctccgac acctactgta gcaatcaatc ctagctcttc tttaatattc tcctggatca
     5101 tttgagccag cttctttctc cgttgacttc gtgttccttc agtcgtaaaa agattcagtg
     5161 atcgggtcac ttttaagatt gattcatcga tcgagtaaat cagtagatct tcatcagcca
     5221 catatcttct gaaaatatta tttacctgca tatttcgctt gatatatagg ttcatacgtg
     5281 gtggaacaac gtgtagtgtt ttaggaaatg gttgtggtaa gtcacgtggt ctactcacat
     5341 ttgtaatacc ataccgcttt tttgcttcag gagaagaagc taatatcaat cctgaaccag
     5401 tattatcagc tcgactcata acaacaagtt ctgttgttaa tggatctaaa tttctttcta
     5461 tacattcgac acttgcgtaa aagcttttca tatcgattaa aaagtaatca tttactggct
     5521 cttttgaata atccatcata aataacactc cattctttca cattatacaa acatatgttc
     5581 gatattgtaa agaagaaata gctaaataca taaaaaaaga agcataggta tgctatcacc
     5641 ctgcttcttc ttttgatttg tatgatcaat ttattctatc actttactaa ttaatttgaa
     5701 aatctaataa aaactctcac tctaaaagag tgagagactg caaattagag tacggacttt
     5761 tttggagttc cgttctttaa tattaacaaa tgaataaaat ccttacaaca tttgagaata
     5821 agttatcagt agacacttac aaaaaattct catactattt cctccatcat tagtttttgt
     5881 ctatataagg tttaccctaa atagacactg gtataacatt gaaaaatatg gcttcctcgt
     5941 gtatgattag ggtgtatttt aaatagttgc agaaagtagg taaaagtttt gagagttgca
     6001 tacatacgtg tctcatcgat cgatcaaaat gaacaacgcc agattgaaga aatgaaaaaa
     6061 ttcggtgcag agagaatttt catcgagaaa caatctggcg caacgattac tcatcgacct
     6121 gttttccaag aagcattaga ttacgtccga gatcaagata tttttattgt ggaagctatt
     6181 gatcgacttg gaagaaatta tgacgagatc atcgcttcag tcaattactt gaagaagaaa
     6241 aatgtgaagc tgatcattac tagtttaccg atcatggctg aagcaattgg caatcctctc
     6301 ctggataaat tcattaagga tctgatcatc caaattttgg ccatgatcgc tgaacaagaa
     6361 cggactgaat cgaaacgtcg ccaggcacaa ggaattaaga tcgcaaaagc aaacggcgtc
     6421 tacaaagggc ggcccaaatt atacagtgca gacgcaaaag atccccaacg tcgccttgtt
     6481 tacaaaagca tcgttgaaga tcttaaaaat ggtgttgcga ttgctaagat tgctaaagac
     6541 tacaatgtta cgagacaaac cgtgtataga ataaaaaaag acagcatggt caatgacaaa
     6601 tagttgatca tgctgctttt attatcttct catctttaat ttcctacata taattactat
     6661 ctataaaatt aagtcttact cagaacagat aaagcataaa attccaacaa accatttttt
     6721 acttaactaa ataaagagtc tgttaatata atcttcactt atttacgtta atcccctact
     6781 gtatttgttg gttccttatc tatattttct acaaaatcct tattatcttg tgatttagct
     6841 tcgtataatt ctttgtatgt cgacacattg gttatactaa ttcctatagc tagttcttga
     6901 gactctactt ttctctctag ctcctttgac aaagtgattt gagatttatt tggttccgtt
     6961 ggagtcaatt ctatagattc ttcataaaaa tctctataca taaagctatc attaatttta
     7021 taaaaaaaat taaatgtttt tagcactgcc atataacatt caaaacattc ttctatagtt
     7081 ggtttacttc cttgaggaga atgaaccaat ttattacgtt tttctatgaa atttttaatt
     7141 tcctttctta tttctttcgg aaactcaata ttaaagtacc ctttaaacat gctccctaat
     7201 aatttgtcta cagggggaga ttgtaaattc tcaaatatta gagttaaatc aggtttctct
     7261 tgttgataaa attcctttac ccctatttca caggctgtaa ttatcgaaat aaacctatct
     7321 ctagtatttg atatattttt acttctttta aggagtataa gagaaaaggg gatactttcg
     7381 tgtccatgca tgtaattaac taattgttga gcttcagaag ttatatctct aattatatag
     7441 ttaacggaat taggaagtgt ctttaactct aatgaaattt ctatccaatt actatcttct
     7501 aatctgtata tgctcgaagt ataatttgat tcgtcagcaa gcatcgctac atcccaaaca
     7561 ctgatcataa aatcttttaa acgttcaatc atttcagcaa tattgctaaa atcagctaat
     7621 ttttctttag aataaaaaaa aacatttata tcagaatcaa taaattctat tttttttatt
     7681 actatatcgt catcattatc ttgaccattt atgtttattg ttccctgagt ggggagtact
     7741 tctttcaaat tcaagttaaa tttatattcc attacttctc ctccaatata taaaaattac
     7801 ttaactgtat ccaaatttaa tatgtaaata aatcattaat ttttagctaa attctatgag
     7861 aagtttattt ttgttgtttt tgctaattaa ctaaaactag tacaaaaaat ttaaaataga
     7921 ttattaaacc tattgcttat tctattcagt catatatata agcgtcatcc tttagtaact
     7981 aacatcccac actacaatag tattataaca tatctattca acttcaaatt gttgaaattt
     8041 aagccattta tatttacttt tcctcaactt tagaaatgat tttactaacc ttatatccgt
     8101 aatacgcgta acaaacacat tcattaataa caatcaacac taagctatat tagcttttta
     8161 aatgactagg gtaaataaaa taaggatcaa aaacttttaa gtttctgacc ctttccttac
     8221 ttccgattgg ttaatagtga tcttttgcag aataaataaa tatcgtttca ttttcaactc
     8281 tatatatcag tctatgttca tctgtaattc ttctggacca ttttccagat aaatcatgct
     8341 ttaatggctc aggttttcct aatccagcaa agggggaacg atcgatatct tttattaact
     8401 tgttaatctt ttttatattg cttttgtttc cttgctcatg ccaataaaga taatcatccc
     8461 aagcatcatc agaccaagcc ttaatcatca gattcaacct cgattaagtc atgtgtttta
     8521 aatgcacctt tggagaattg ttcatctcct cgacgaattt tttccatgac gtaattatta
     8581 gaaagtgttc tcaacgtttc ttgcatagaa tcataatctc tttttgataa tacaacaact
     8641 gtatcttcta catctttact tgttacaata agtgtttcag catcctcatt aacttgtttc
     8701 atataactac gtaaattttg gcggaaattt gaataagcta ctgcttccat tcctttcacc
     8761 ccttatttat ttctataaaa caattgtaca ttatattgta caattaagca aatgattaat
     8821 attctaactt cttataatta aaagtgctat cacttttagt ttttaggata aaaagtgacg
     8881 tcactttttg agtgctggta cctcaaatcc tgctttttct tgggcaatca ataaatctaa
     8941 cgcaatacac ataatcctgg attcactttt gccgtacttc tgctttagtt gctgcaattc
     9001 tttttgtctc ttttcatcca acgtaacggg taaccgttta tttcctattg caattggcat
     9061 tagtactctc tcctttcatc gaaataaccc tatgaaaaat atggctgcaa tcatcttgaa
     9121 tagtagactc acaagggcta atacaaagcg ccaaaagatc aaacaaaata ccagtattgc
     9181 caaaaatact acgaagttta aatgcacagc tgcagcatgg cgcataagta tttcgccaac
     9241 aaataggact aacaatagag gaatgatttt attcatatgg atctccttct ttctcttagg
     9301 ttatcgggtg ttgcccgctt atttcgcacc acctaagcgg cgggggcggt tggagcgatt
     9361 ggtgcgaaat aagaaccatt cccttagtta ctaggttttt tgtccttatt tcacacattc
     9421 tcacttttcg gtataaatga ttattgcctt tttttccttt caaatcgttg atatggcatt
     9481 cgtttacggt caagttcatt ttggatttct tctgtatcgt cgta
//
LOCUS       DACGLE010000092         8959 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0534
            SAMEA3304449-rid9560453.denovo.097, whole genome shotgun sequence.
ACCESSION   DACGLE010000092 DACGLE010000000
VERSION     DACGLE010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304449
            Sequence Read Archive: ERR985064
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8959)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8959)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702225.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702225.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 20:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,045
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 27 of 67
            Pseudo Genes (incomplete)         :: 25 of 67
            Pseudo Genes (internal stop)      :: 19 of 67
            Pseudo Genes (multiple problems)  :: 4 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8959
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304449-rid9560453.denovo.097"
                     /strain="VREN0534"
                     /db_xref="taxon:1352"
     gene            complement(<1..360)
                     /locus_tag="IXT20_002455"
     CDS             complement(<1..360)
                     /locus_tag="IXT20_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate transporter"
                     /protein_id="HAQ4860877.1"
                     /translation="MATVWMGLALVGNTFSPNSAMEGQGVIDVLTHIYTTGPAEYAKS
                     ILVNVFFGAFFGRVLVDSGIAATLIRKVVELGGDKPRITMSLLCIVTAVIFMSMTGIG
                     PVISIAVIVLPILMSLGI"
     gene            complement(712..1071)
                     /gene="rplT"
                     /locus_tag="IXT20_002456"
     CDS             complement(712..1071)
                     /gene="rplT"
                     /locus_tag="IXT20_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HAQ4860878.1"
                     /translation="MARVKGGTVTRKRRKRVLKLAKGYYGSKHTLFKTAKEQVMNSYN
                     YAYRDRRQKKRDFRKLWIARINAAARMNGLSYSKLMHGLKVAEIDINRKMLADLAVND
                     AAAFTALADQAKEALAK"
     gene            complement(1124..1324)
                     /gene="rpmI"
                     /locus_tag="IXT20_002457"
     CDS             complement(1124..1324)
                     /gene="rpmI"
                     /locus_tag="IXT20_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001125942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HAQ4860879.1"
                     /translation="MPKQKTHRGSAKRFKRTGKGGLKRFRAFTSHRFHGKTKKQRRQL
                     RKASMVSSGDFKRIRQQLAKMK"
     gene            complement(1396..1920)
                     /gene="infC"
                     /locus_tag="IXT20_002458"
     CDS             complement(1396..1920)
                     /gene="infC"
                     /locus_tag="IXT20_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HAQ4860880.1"
                     /translation="MTIAKDMMVNDGIRARELRLIGSDGEQLGVKTKAEALNIAEQAN
                     LDLVLVAPGAKPPVARIMDYGKYRFEQQKKDREARKKQKVINVKEVRLSPTIDLNDFN
                     TKLRNARKFLEKGDKVKASIRFKGRAITHKEIGQKVLNRLAEETADIATVEQKAKMDG
                     RSMFLTLAPKNDSK"
     gene            2240..2872
                     /locus_tag="IXT20_002459"
     CDS             2240..2872
                     /locus_tag="IXT20_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HAQ4860881.1"
                     /translation="MKKIEPIPPSLPLLTKGEFYLEDEEIFSGLLINGGNQSYLDAKN
                     MVLRESHIQKLTMQKTGLRHFECSNVIFEKCDFSNLEWIGGSFHQVVFHQCKLTGTNF
                     AESYLRDCTFTDCIASMASFSSTNLKAVSFDHCQLEDSEFYEVTWKNLFLSDNQLTGS
                     NWFRTSLKGLDFTTNFFTTIALSQDSLAGLIVNQEQALVIAQALGLQIKD"
     gene            complement(2874..3779)
                     /locus_tag="IXT20_002460"
     CDS             complement(2874..3779)
                     /locus_tag="IXT20_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HAQ4860882.1"
                     /translation="MREKHYFNQDRNFWINVQQNDVLAVKNLKEEYGISDEMLTYSLD
                     KNERARVEYDSFDEALLLVSNVPHQQKVDNHYETSPIAFILKEDGLFTFTTPNTEYVI
                     RLIRSLLERMPDMSVYSLLFRTLFLISDSFFPLIEEVNSERQRLNLKLREKTTNKNLL
                     QLSDLEIGLVYLVTGTKQNVVLLEQIKALAIYRKLSEKEKEELDDALIEARQAVEMTN
                     LASQILDQLSGTYNNLLNNNLNDTMKFLTVWSLILTVPTIVTGFFGMNLQLPFTHSVF
                     GWEIALIISLVLSIWMLIALWRRIR"
     gene            complement(3864..4517)
                     /locus_tag="IXT20_002461"
     CDS             complement(3864..4517)
                     /locus_tag="IXT20_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HAQ4860883.1"
                     /translation="MIVLAGTIGAGKSSLTEMMAEHFDSQAFYESIDDNEVLPLFYAN
                     PEQYAFLLQIYFLNKRFASIKQAMKDDNNVLDRSIYEDSLLFHLNADLGRATETEVCV
                     YDELLENMMEELPYAAHKKHPDLLVHIRVSFDTMLERIEKRGRSYEQLSFDPSLYDYY
                     KELNRRYDQWYEEYKESPKIQIDGDRYNFVEDPQAKEEVLKMIEEKLAEIRQTKVSI"
     gene            4839..5783
                     /gene="mvk"
                     /locus_tag="IXT20_002462"
     CDS             4839..5783
                     /gene="mvk"
                     /locus_tag="IXT20_002462"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HAQ4860884.1"
                     /translation="MANYGQGESSGKIILMGEHAVVYGEPAIAFPFYATKVTAFLEEL
                     DAMDDQLVSSYYSGNLAEAPHALKNIKKLFIHLKKQHDIQKNLQLTIESTIPAERGMG
                     SSAAVATAVTRAFYDYLAFPLSREILLENVQLSEKIAHGNPSGIDAAATSSLQPIYFT
                     KGHPFDYFSLNIDAFLIVADTGIKGQTREAVKDVAHLFETQPHETGQMIQKLGYLTKQ
                     AKQAIIENSPETLAQTMDESQSLLEKLTISNDFLNLLIQTAKDTGALGAKLTGGGRGG
                     CMIALAQTKTKAQEISQALEDAGAAETWIQGLGVHTYV"
     gene            5776..6753
                     /gene="mvaD"
                     /locus_tag="IXT20_002463"
     CDS             5776..6753
                     /gene="mvaD"
                     /locus_tag="IXT20_002463"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HAQ4860885.1"
                     /translation="MFKGKARAYTNIALIKYWGKKNEELILPMNNSLSLTLDAFYTET
                     EVIFSDSYMVDEFYLDGTLQDEKATKKVSQFLDLFRKEAGLSLKASVISQNFVPTAAG
                     LASSASGLAALAGACNTALKLGLDDLSLSRFARRGSGSACRSIFGGFVEWEKGHDDLS
                     SYAKPVPSDSFEDDLAMVFVLINDQKKEVSSRNGMRRTVETSSFYQGWLDSVEGDLYQ
                     LKQAIKTKDFQLLGETMERNGLKMHGTTLAAQPPFTYWSPDSLKAMDAVRQLRKKGIP
                     CYFTMDAGPNVKVLVENSHLSEVQETFTKLFSKEQVITAHAGPGIAIIE"
     gene            6766..7851
                     /locus_tag="IXT20_002464"
     CDS             6766..7851
                     /locus_tag="IXT20_002464"
                     /EC_number="2.7.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomevalonate kinase"
                     /protein_id="HAQ4860886.1"
                     /translation="MIEVSAPGKLYIAGEYAVVETGHPAVIAAVDQFVTVTVESARKV
                     GSIQSAQYSGMPVRWTRRNGELVLDIRENPFHYILAAIRLTEKYAQEKNILLSFYDLK
                     VTSELDSSNGRKYGLGSSGAVTVATVKALNVFYALNLSQLEIFKIAALANLAVQDNGS
                     CGDIAASCYGGWIAFSTFDHPWLQEQETQHSISELLALDWPGLSIEPLIAPEDLRLLI
                     GWTGSPASTSDLVDQVHRSREDKMVAYQLFLKNSTECVNEMIKGFKENNVTLIQQMIR
                     KNRQLLHDLSAITGVVIETPALNKLCNLAEQYEGAAKSSGAGGGDCGIVIVDQKSGIL
                     PLMSAWEKAEITPLPLHVYSDQRKENR"
     gene            7848..8912
                     /locus_tag="IXT20_002465"
     CDS             7848..8912
                     /locus_tag="IXT20_002465"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HAQ4860887.1"
                     /translation="MNRKDEHVSLAKAFHDKQKNEFDFVRIIHNPLPQIAVSDVDLST
                     QAVGFTLSSPFYINAMTGGSEKTKKINQDLAIVAREADLMIATGSVSAALKDPSLADT
                     YTIMRQEYPHGKIIANIGAGTSVERAQEAIRLFHADALQIHLNAPQELVMPEGDRDFT
                     NWKVLIQETQTAIDVPLIVKEVGFGMTRETLNDLAALGVHTVDISGRSGTSFTQIENA
                     RRSKRELSYLADWGQSTVSSLLEANEADTSMEILASGGIRNAYDIFKALCLGANAVGT
                     SGTVLTHLMNHGVEETIILMKQWQEELRLLYTMVGATNTAALHQQSLIFSGPVKDWCE
                     ARGIDLVKYGRRTEKSVGQK"
BASE COUNT         2694 a         1859 c         1736 g         2670 t
ORIGIN      
        1 aatacctaat gacatcaaga tcggcaacac aataactgca atagaaatca ctggtccgat
       61 tcctgtcatc gacatgaata taacagcggt tacaatacat agtaagctca tcgtgattct
      121 tggcttgtcc cctccaagtt caactacctt tcgaatcagg gtagctgcga ttccgctatc
      181 tactagaaca cgcccgaaaa aggcaccaaa gaagacattt accaagatcg atttggcgta
      241 ctcagctggc cccgtcgtat agatatgcgt caacacatca attactcctt gcccttccat
      301 cgccgagttt ggtgaaaacg tgtttccgac tagcgctaat cccatccata cagtagccat
      361 gatgacaaaa gccgaccatc aagttgtagc cttttacaca gtaccagacc atgaaaaaga
      421 atgagataac taacaaaata ccaataataa tatcactcat gttattcctc cgtccatttt
      481 gtttttctga taagcaaact aatatatcag tcaaactcat tctaacttat gtagttagcg
      541 ctttcattaa aggatgacca taaatgttca gttttcaatt aaaaaacctt aattatgtta
      601 ttttagactt atacgttatt gtcctgtttc atttgatgga ggattgtaaa taaaaaaaga
      661 ggaaagaaca taacttttcg ttatcttctt tcctctttat aattgtttag cttatttagc
      721 caaagcttct tttgcttggt cagccaatgc agtaaatgct gctgcatcgt taacagccaa
      781 gtcagctaac attttacggt tgatgtcgat ttcagcaact ttcaaaccgt gcattaattt
      841 tgagtagctc aagccattca tacgagctgc tgcgttgata cgagcaatcc acaatttacg
      901 gaagtcgcgt ttcttttgac gacgatcgcg atatgcgtag ttgtatgaat tcattacttg
      961 ttcttttgct gttttaaata aagtgtgttt tgaaccgtag taaccttttg ctaattttag
     1021 cactctttta cgacgtttac gagttactgt tccaccttta acacgtgcca tgtataattc
     1081 ctcctaataa aaataaatct tcagattctg ttatttgtac ttcttatttc atttttgcta
     1141 gttgttggcg aatacgtttg aagtcgccac tagaaaccat gcttgcttta cgcaattgac
     1201 ggcgttgttt ttttgttttg ccgtggaaac ggtgacttgt aaacgcgcgg aagcgtttca
     1261 atccgccttt accagtacgt ttgaaacgtt ttgctgatcc gcggtgtgtt ttttgttttg
     1321 gcatgactat atttcctcct caaaatcttt cgttatccga ctatcggctg tccaacagcc
     1381 agttagtcat tcaaattact tactgtcgtt tttcggtgcc agcgtcaaga acatgctgcg
     1441 tccgtccatt ttcgcttttt gttccactgt tgcaatatcc gcagtttctt cagctaagcg
     1501 attcaagact ttttgaccaa tttctttatg ggtaatggca cggcctttga aacggataga
     1561 agctttcact ttatcgccct tttccaagaa cttacgtgca ttacgaagtt ttgtattgaa
     1621 gtcattcaag tcgatcgtag gacttaagcg aacttctttt acattgatca ctttttgttt
     1681 tttacgcgct tcgcggtctt tcttttgttg ttcgaaacgg tatttaccgt agtccatgat
     1741 tcgcgcaact ggtggtttcg caccaggggc aactaacaca agatccaagt ttgcttgttc
     1801 tgcaatattc aatgcttctg cttttgtctt aactcctaat tgctcaccat ctgaaccgat
     1861 caatcgtaac tctcgtgcac gaatgccgtc gttaaccatc atatcctttg ctatggtcat
     1921 tcacctccaa atattatgag agaaagaaaa gcttaccgaa taaacggcaa taaaaaaacg
     1981 agttctctta cagaacccgc gcgtacttca cctaatagaa ccagtgaaaa ctatctagcc
     2041 cagtgacatt atcaactaag gcgagaagcg ggtgcttctg cttttatttc tcaaccatta
     2101 tagtatagca aaaactaatc tttttgtcaa tgtttttcat acagtttgtc ttccttttct
     2161 gaaaatgatt agattagcag gctttctagc agtgattccc gcttctttgc tatactaaag
     2221 aaaaacgagg tgaacccaca tgaaaaaaat cgaacctatt ccaccttctc ttcctcttct
     2281 tacaaaggga gagttttatt tagaagatga ggagatcttc tctggtcttc tgatcaacgg
     2341 aggtaaccaa agctatctcg atgccaaaaa tatggtatta agagagagtc atatccaaaa
     2401 acttacgatg caaaaaacag gtttacgtca ttttgaatgc agtaatgtca tttttgaaaa
     2461 atgtgatttt tccaatctgg agtggattgg tggcagtttc caccaagtcg tgtttcatca
     2521 atgtaaatta acaggtacga attttgccga aagttatctt cgagactgta ctttcactga
     2581 ttgtattgct tcgatggctt cctttagtag tacaaacttg aaagctgtct ccttcgatca
     2641 ttgtcaatta gaagacagcg aattttatga ggtcacttgg aaaaaccttt ttttgtctga
     2701 caatcagtta accggttcga actggtttcg aacttctctg aagggattag actttacgac
     2761 taatttcttc acaacaatcg ctttatctca agattctttg gcgggactaa tcgtcaatca
     2821 agagcaagca cttgtgatcg cccaagccct tggtttgcag atcaaagact agatcaacga
     2881 atccgccgcc acagtgcaat aagcatccaa atagataaga ccaaactgat gatcaaggcg
     2941 atttcccatc cgaaaacgga atgggtaaag ggtaactgga gattcattcc gaagaatcct
     3001 gtcacaatag ttggaacggt caagatcagc gaccagaccg tcaaaaattt catcgtgtca
     3061 ttcaagttgt tattcagcaa attattataa gtgccagaga gctggtctaa aatctgcgaa
     3121 gctaagttcg tcatttcaac tgcttgtcgt gcttcgatca gtgcatcgtc caactcttct
     3181 ttttcctttt ctgacaactt gcgataaata gccagtgcct tgatttgttc taaaagtact
     3241 acattttgtt ttgttcctgt aactaagtaa accaaaccga tctctaaatc tgaaagttgc
     3301 agcagattct tgttcgttgt tttttctctt aatttcagat taagccgctg acgttcacta
     3361 ttcacttcct cgattaaagg aaaaaaggaa tcagaaatca aaaacaatgt acgaaacaat
     3421 aaactgtata cactcatgtc tggcatccgt tccaaaagcg atcgaattag tcgaatcacg
     3481 tactcggtat tcggcgtggt aaatgtaaac aaaccgtctt cttttaaaat aaaagcgata
     3541 ggactagttt cataatgatt atcgactttt tgctggtgcg gaacatttga tacaagcagt
     3601 aacgcttcat caaaagaatc gtactctacg cgcgcccgtt catttttatc tagagaataa
     3661 gtcagcatct cgtcactaat gccatattct tcttttaagt ttttaacagc tagcacatcg
     3721 ttttgctgta cattgatcca aaaattacgg tcttgattaa aatagtgttt ttcccgcata
     3781 ctctatcctc ctgacatttg tttccagtat aaagaaaaaa gtcgctcaaa taaaggttta
     3841 ccctttattt gagcaactgt aaatcaaatg gatactttgg tttggcgaat ctctgctaat
     3901 ttttcttcga tcattttcaa gacttcttct tttgcttgag gatcttccac aaaattatag
     3961 cggtctccat cgatttggat ttttggactt tctttgtatt cttcatacca ttgatcataa
     4021 cgtctgttca attctttgta ataatcgtat agacttgggt caaaggacaa ttgttcatac
     4081 gaacgaccac gtttttcgat acgctctaac atcgtatcaa aagatacacg aatatgaact
     4141 aataaatccg gatgtttttt atgggcagca tagggaagtt cttccatcat gttttccagc
     4201 agttcatcat acacacaaac ttctgtttct gttgctcttc ctagatctgc atttagatgg
     4261 aaaagcagtg aatcttcata aatcgaacgg tccaatacat tattgtcatc tttcattgct
     4321 tgtttgatgc ttgcaaaacg tttattcaaa aaataaattt gcagcaaaaa agcatactgt
     4381 tcagggtttg cataaaataa gggcaagact tcattatcat caatcgattc ataaaatgcc
     4441 tgactatcaa agtgttcagc catcatttct gttaaacttg attttccggc accaattgta
     4501 cctgctaaaa cgatcacgct catttacctc cagctcactt catcactaaa tattgtgttt
     4561 aactccaacc ttttttaaat atacccccaa tatctagtag tgtcaattgt attttttcta
     4621 gtcaaagaag aaaaaaagtc aaaaaattct taagaaaaaa gtgttttttc tttagaaaac
     4681 gtattcttag ttttacttct gagatggttt tagtaaaata aaggaggaat gttttttgag
     4741 acccttagtg gacaaaaaaa ttcgtgaagc agcgcaactc gttttgggcg ctttattttt
     4801 tgcttaaaaa tcacttttac gtgagaagga gttagattat ggcaaactat ggccaaggag
     4861 aatcaagcgg aaagatcata ttgatgggcg agcacgcggt tgtttatgga gaaccagcga
     4921 ttgcctttcc tttctatgca acaaaagtca ccgcattcct tgaagagctg gatgcaatgg
     4981 acgatcaact ggtttcttcc tactattcag gaaatttagc cgaagctcct catgcattaa
     5041 aaaatatcaa aaaattattc attcacttaa aaaaacagca tgacatccaa aaaaacttgc
     5101 aactgaccat tgaaagcacg attcctgctg aacgtggaat gggatcaagc gctgcagtcg
     5161 ccacagcagt cactcgtgct ttttatgatt acttagcatt tcctttgtct cgtgaaatac
     5221 tattagaaaa tgtccagctt tcggaaaaaa tcgcccacgg taatcctagt ggaatcgatg
     5281 cagccgctac tagcagcttg cagccgattt attttacaaa agggcatcct tttgactact
     5341 tttctttgaa catcgatgct tttttgattg tcgctgatac aggaatcaaa ggacaaacaa
     5401 gagaagccgt caaagatgtc gctcacctct ttgaaaccca gcctcatgaa actggacaaa
     5461 tgatccaaaa attaggatac ttgacgaagc aagcaaaaca agcaatcatt gagaattcac
     5521 cagaaacgtt ggcacagaca atggatgaat cacagtcact tctggaaaag ctgacaatca
     5581 gcaatgattt tcttaatcta ttgatccaaa cagcaaaaga taccggggca ttgggtgcca
     5641 aattaactgg cggtggacgc ggtggctgta tgattgcatt agcacaaaca aaaacaaaag
     5701 cccaagaaat cagtcaagca cttgaagatg cgggtgctgc tgaaacttgg atacaaggat
     5761 taggagtaca tacctatgtt taaaggcaaa gcacgcgcat ataccaacat tgctctaatc
     5821 aaatattggg gtaagaaaaa tgaagaactc atccttccaa tgaacaacag cctttcgtta
     5881 acattggatg cattttatac agagacagaa gtcatctttt ctgatagcta tatggtagat
     5941 gaattttatc tagatggcac cttacaagac gaaaaagcaa caaaaaaagt cagtcagttt
     6001 cttgaccttt ttcgtaaaga agccggcctc tcactaaaag cttcagtaat cagtcagaat
     6061 ttcgttccta ccgcagctgg attagcctcc tctgccagcg ggctagctgc tttagcagga
     6121 gcttgcaata ctgctcttaa gcttggatta gacgatctct ctctttcaag atttgctcga
     6181 cgcgggtctg gttcagcttg ccgaagtatt ttcggtggtt tcgttgaatg ggaaaaaggc
     6241 catgacgact taagttctta cgctaagcca gtcccttccg attctttcga agacgattta
     6301 gcaatggttt tcgttttgat caacgaccag aaaaaagaag tgtccagcag aaatgggatg
     6361 cgtcgaacag tcgaaacatc cagtttttat caaggctggt tagattccgt tgaaggggat
     6421 ctatatcaat tgaaacaagc aatcaaaaca aaagatttcc aacttctcgg agaaacgatg
     6481 gaaagaaacg gactaaaaat gcatggaacg actttagctg cccagcctcc attcacttac
     6541 tggtctcctg acagcttaaa agcgatggat gcggtgcggc aattgagaaa aaaaggcatt
     6601 ccatgctact ttacgatgga tgctggccct aatgtcaaag ttttagtgga aaacagccat
     6661 ttatctgagg tacaagaaac atttaccaaa ttatttagca aagaacaagt aattactgcc
     6721 catgctggtc caggaattgc gattattgaa taaagggaga tttttatgat tgaagtatct
     6781 gcaccaggca aactctatat tgccggagaa tatgcagttg ttgaaacagg acatccagca
     6841 gttatcgctg cagtcgatca attcgtaaca gtcactgtag aatccgcacg aaaagtcgga
     6901 agtatccaat ctgcacaata tagtgggatg cctgtacgtt ggacaagacg caacggagaa
     6961 ttagttttag atattcggga aaatcctttt cattatatcc ttgctgctat tcgtttgact
     7021 gaaaagtatg cacaagaaaa aaacattctt ttatcctttt atgatctgaa agtaacaagt
     7081 gagttagaca gttcaaatgg ccggaaatat ggattgggtt ctagcggtgc cgtgaccgtt
     7141 gcaacggtca aagccttgaa tgttttttac gcgttgaatt tatctcaatt ggagattttc
     7201 aagattgcag cactagccaa tttagcagtt caagataatg gttcctgcgg cgacatcgct
     7261 gctagctgtt atggtggctg gatcgctttc tcaaccttcg accatccttg gctccaagaa
     7321 caagaaactc agcattctat cagtgagtta cttgccctgg attggccagg tctatccatt
     7381 gagccattga ttgctcctga agatttacgt ttattgattg gttggacggg tagccctgcc
     7441 tctacttctg atttggtcga tcaagttcac cgttcgagag aagacaaaat ggtggcttat
     7501 cagcttttct taaaaaacag tacagaatgt gtcaacgaaa tgatcaaagg gtttaaagaa
     7561 aataatgtaa cgttgatcca acagatgatt cgaaaaaacc gacaattact gcatgattta
     7621 tctgcaatca ctggggtcgt catcgaaacg cctgctttga acaaattgtg taatttagct
     7681 gaacagtatg aaggagccgc aaaatcttct ggtgcaggtg gtggcgattg cggaatcgta
     7741 attgttgacc agaaatctgg catccttcct ttaatgagtg catgggaaaa agcagaaatc
     7801 actccactgc cgttacatgt ctatagcgat caaagaaagg aaaaccgatg aatcgaaaag
     7861 acgaacatgt ttcactagct aaagcctttc atgataaaca aaaaaacgaa tttgacttcg
     7921 tacggatcat ccataaccct ttgccgcaaa tagcagtttc tgatgtcgat ctaagtacac
     7981 aggcagttgg gtttacacta agcagcccat tttatatcaa tgcgatgact ggcggaagcg
     8041 aaaaaacaaa aaaaatcaac caagatcttg caatcgtcgc aagagaagca gatttgatga
     8101 ttgccactgg ttcagtcagc gctgccttga aagacccttc tttagccgat acttatacga
     8161 ttatgcgaca agaatatcct cacgggaaaa tcattgccaa catcggtgca gggacttctg
     8221 tcgaaagagc ccaagaagct atccgattgt ttcacgcaga tgccttacag attcatttga
     8281 atgcacctca agagttggtt atgcctgagg gagaccgtga ttttaccaat tggaaagtac
     8341 ttattcaaga gactcaaaca gccattgacg tccccctcat cgtaaaagaa gtcggttttg
     8401 gcatgacaag agaaacgctc aatgatctag ctgctttagg agttcataca gttgatatca
     8461 gcggtcgaag cggaacaagc ttcacccaga tcgaaaacgc ccgtcgctcc aagcgggaac
     8521 tgagttattt agctgactgg gggcaatcga cagtctcttc attgcttgaa gcaaatgaag
     8581 cagacacttc tatggaaatc ctagcctccg gaggtatccg caatgcctat gacatattta
     8641 aagccctttg tctcggcgca aatgcagtgg gtacttcagg aaccgtactg acgcatctga
     8701 tgaatcacgg cgtagaagaa actattatac tgatgaagca atggcaagaa gagcttcgtc
     8761 tgctttatac tatggtcggc gcaacgaata ccgcggccct acaccaacaa tcactgatct
     8821 tttccggacc agtgaaggat tggtgcgaag caagagggat cgacttagtg aagtatggaa
     8881 gacgcacaga aaagagcgtg ggacaaaagt aaagaacact tttatcccac gctctaaaca
     8941 cgtataaacg gcggaagca
//
LOCUS       DACGLE010000093         8734 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0534
            SAMEA3304449-rid9560453.denovo.098, whole genome shotgun sequence.
ACCESSION   DACGLE010000093 DACGLE010000000
VERSION     DACGLE010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304449
            Sequence Read Archive: ERR985064
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8734)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8734)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702225.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702225.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 20:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,045
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 27 of 67
            Pseudo Genes (incomplete)         :: 25 of 67
            Pseudo Genes (internal stop)      :: 19 of 67
            Pseudo Genes (multiple problems)  :: 4 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8734
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304449-rid9560453.denovo.098"
                     /strain="VREN0534"
                     /db_xref="taxon:1352"
     gene            2..74
                     /locus_tag="IXT20_002466"
     tRNA            2..74
                     /locus_tag="IXT20_002466"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:35..37,aa:Asp,seq:gtc)
     gene            78..150
                     /locus_tag="IXT20_002467"
     tRNA            78..150
                     /locus_tag="IXT20_002467"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:111..113,aa:Phe,seq:gaa)
     gene            155..225
                     /locus_tag="IXT20_002468"
     tRNA            155..225
                     /locus_tag="IXT20_002468"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:187..189,aa:Gly,seq:tcc)
     gene            238..311
                     /locus_tag="IXT20_002469"
     tRNA            238..311
                     /locus_tag="IXT20_002469"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:272..274,aa:Ile,seq:gat)
     gene            323..394
                     /locus_tag="IXT20_002470"
     tRNA            323..394
                     /locus_tag="IXT20_002470"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:356..358,aa:Glu,seq:ttc)
     gene            660..1853
                     /locus_tag="IXT20_002471"
     CDS             660..1853
                     /locus_tag="IXT20_002471"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HAQ4860888.1"
                     /translation="MVERHLFTSESVSEGHPDKIADQISDAILDAILKQDPTARVACE
                     TSVTTGLVLVFGEISTTAYVDIQKVVRETIKEIGYTRAKFGFDGDTAAVLVAIDEQSP
                     DIAQGVDEALEIRDEDKKDVLDEIGAGDQGLMFGFAVDETPELMPLPIALSHRLVRRL
                     ADLRKSNELTYLRPDAKSQVTVEYDDQGQPERVDTIVISTQHDDAVDNETIRHDVIEK
                     VVKEVIPAELLDDQTKYYINPTGRFVIGGPQGDAGLTGRKIIVDTYGGYARHGGGAFS
                     GKDATKVDRSASYAARYIAKNIVAAGLARKAEVQLAYAIGVAQPVSISINTFGTGTVP
                     EEELIAAVRENFDLRPAGIIEMLDLRRPIYKQTAAYGHFGRTDVDLPWEHTDKVDALK
                     ASLAK"
     gene            1983..3455
                     /locus_tag="IXT20_002472"
     CDS             1983..3455
                     /locus_tag="IXT20_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ4860889.1"
                     /translation="MKKETNVTLVTVSIFIATFMTAIEGTIVTTAMPTIVGSLHGMEI
                     MNWVFSIYLLTNAMLTPIYGKLADKVGRKPIFIFGTLLFIIGSLFCGLSQSMIGLIAA
                     RAIQGMGAGAMMPVALTIIGDLYSIEKRAKVLGLNSSAWGIASVFGPLAGGFIVETIS
                     WHWIFFINVPIGILLIILIWLYLNEPKIIHESKPMDIFGSFSLMAMLVSLLLGFQLLG
                     DEGMSPLVSALLLGSIVMLGIFVFAEKRAKDPVIMMHLFKNPLFVVVNLAAALASGFL
                     MGIDVYIPMWMQGVLGLNAAIGGLVLAPLSLVWMVGSFLASKWMTQMKTHWVLRIGLL
                     IAAIGGAWLYLMPFRSNYLWFFAISTVIGIGLGVTMTTSTVSAQNSVSRDQLGVATSF
                     NTLVRTIGQTVMVSIFGLLINGVTKRELAAANLTNDADIMNKLVNPQTAKYIDADILL
                     PLRKILYDGLHSVYLVGLLLVIIALVLTLFVHEKSGKIKQ"
     gene            3538..4326
                     /locus_tag="IXT20_002473"
     CDS             3538..4326
                     /locus_tag="IXT20_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HAQ4860890.1"
                     /translation="MAFIQANIYSNVLEMEVNLNVILPQTTKKVIGTSSEQETTDIPV
                     LYLLHGMGGNHSVWERRTSIERYVANYGLAVIMPSTDLGWYTDTVYDMKYWTFIAEEL
                     PEICHELFPQLTRKREKTYAAGLSMGGYGALKLGLAKSENFAAVISLSGAVSVGNRMD
                     DLLMVRKASFWEGIFGPLDQIEGSKNDPQYLLKELVKSGKPVPRFYMACGESDFLYQA
                     NQEMVSAMQEQGLAVTFEQGPGDHNWIFWDQWIQRALAWLMEEK"
     gene            complement(4393..5061)
                     /locus_tag="IXT20_002474"
     CDS             complement(4393..5061)
                     /locus_tag="IXT20_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HAQ4860891.1"
                     /translation="MEIKLSQAIIIQAAFDILAETQALSQLSMRNIANRIHVQAPALY
                     WYFKNKQQLLQKMAETMEEELALPDPTLPWNEQLLQYMENYYDLYTRFPCGAELEINT
                     VPAFPSRLEHLEQMIQLLVREGFSVTQSHQAIASLHNLLIGQLMDQQHEQQLRQKIMD
                     GNEFLKETVLFMRNYVVENQLEGFKESMQSHAELNEKALFLTSAQTYIEGLCQQNKRR
                     ENEQ"
     gene            5176..6888
                     /locus_tag="IXT20_002475"
     CDS             5176..6888
                     /locus_tag="IXT20_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ4860892.1"
                     /translation="MKLILRHAKKYKVAVFISLLSVAVMVAAALWQPKLLQQVLEAII
                     TEDNDEMKTIGIYLISLALLGLAAGVTNTIFSAKVAQGVSADIREEAFRKIQTFSFGN
                     IEQFSAGNLVVRLTNDITQIQNLVMISLQSLFRIPFLFIGAFILAMITMPQLWWIIVA
                     LIVTVFLITALSFTRMGKHFMIIQKLIDRVNSIAKENLLGIRVVKSFVQEENQLKSFS
                     KVSEDLTKHNIIVGTLFSVMIPSFMLAANLAVVGAIFFVSDLVKDDPTLIGGIASFMN
                     YLMQIMMAIIIGGMMMMMTSRAAVSLKRIAEILDAEPDLTYLDVPEQELTGSVTFDHV
                     SFRYPGEDTDTLKDISFSIKPGEMVGIVGATGAGKSTLAQLIPRLFDPTEGKVEVGGV
                     DLRQVNEKSLRQTVSFVLQKAILFSGTIAQNLRQGKKNATEKEMEHASSIAQAKEFIE
                     KLSDRYEAPIEERSSNFSGGQKQRLSITRGVIGNPKILILDDSTSALDARSEKLVREA
                     LDRDLKGTTTIVIAQKIASVVKADRILVLDEGRLVGEGTHEELVAGNRVYQEIFETQK
                     GTEE"
     gene            6891..8663
                     /locus_tag="IXT20_002476"
     CDS             6891..8663
                     /locus_tag="IXT20_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ4860893.1"
                     /translation="MTDLIKASKFFYHYLKRYKLSFFFIFITVIIATYLQVKAPQYIG
                     EAFQELANYVGGLMQGVDDKSKFVSVIWKLLLFYVLASAANFIYSILFTQVVGKSTNR
                     MRIGLFNKLEKLTIRFFDSHQDGEILSRFTSDLDNIQNSLNQALLQVITNAVLLVGIL
                     IMMFRQNVELAWATIASTPVAVLIAVLIIRKARKYVDLQQDEVGKLNGYMDEKISGQR
                     VIITNGLQEETIEGFLEHNQSVREATFKGQAYSGLLFPMMQGMSLVNTAIVIFFGGWL
                     ALNGDLERSVALGLVVTFVQYSQQYYQPLMQISSGYSMIQLAITGARRLNEIFDEKDE
                     VKPENGKAFDGVEKGLALQHVDFGYSPEKLVLKDVSINVDKGEMVALVGPTGSGKTTI
                     MNLLNRFYDVNDGSVVIDGIDIRDYDLDALRRHVGIVLQESVLFSGTIKDNITFGKPD
                     ASDEEVINAAKQANIHDFIMTLEKGYDTEISEENNLFSTGQKQLISIARTIITNPSLL
                     ILDEATSNVDTVTEARIQKAMDEAIKGRTSFVIAHRLKTILNADRIVVLRDGEVIEEG
                     SHHELLAENGFYAELYHNQFVFES"
BASE COUNT         2624 a         1487 c         1945 g         2678 t
ORIGIN      
        1 tggttccgtg gtgtaggggt taacatgcct gcctgtcacg caggagatcg cgggttcgat
       61 tcccgtcgga accgtttggc tcggtagctc agttggtaga gcaatggatt gaagctccat
      121 gtgtcggcag ttcgattctg tcccgcgcca ttatgcgggt gtagtttagt ggtaaaacca
      181 cagccttcca agctgttgtc gcgagttcga ttctcgtcac ccgcttttag tttttatggg
      241 cctatagctc agctggttag agcgcacgcc tgataagcgt gaggtcgatg gttcgagtcc
      301 atttaggccc atttatttta ttggcccgtt ggtcaagcgg ttaagacacc gccctttcac
      361 ggcggtaaca cgggttcgaa tcccgtacgg gtcatggaag atactttttg agtatctttt
      421 tctttttatc tgaaagaatg attttaaaaa gcttagcaga gttatcttta tggatgattg
      481 tgctaagctt ttttggacag ttctaatgta aaatattttt atatttttta agcaaaaaaa
      541 tgaaaaaata cgagcagaaa actgttttat ctgctatact tggttcgttg caagttcccg
      601 aaaggatccg caggacggag atgccttgta accgaattta actaggggga atcattataa
      661 tggtagaaag acacttattt acatcagaat ctgtttctga aggacatcca gataaaattg
      721 ctgaccaaat cagtgatgca atcttggatg caattttaaa acaagatcca acagcacgag
      781 tagcgtgtga aacgtctgta acaactggtc ttgttttagt atttggagaa atatctacga
      841 cggcatatgt agatattcaa aaagtcgtac gggaaacaat aaaagaaatt ggttatacac
      901 gtgcaaaatt cggatttgat ggagatacag cagctgtatt ggttgcgatt gatgaacaat
      961 ctcctgatat tgctcaagga gttgatgaag cacttgagat ccgggatgaa gataaaaaag
     1021 acgtattaga tgagattggt gctggtgacc aaggtttaat gtttgggttt gctgtagatg
     1081 aaacaccaga attgatgcct ttaccaatcg ctttgagtca tcgtctggta cgacgcttgg
     1141 cagatttgcg taaatcaaat gaattaacgt atttacgtcc agatgcaaaa tctcaagtaa
     1201 cggttgaata tgatgatcaa ggacaaccgg aacgcgtaga tacaatcgtt atttcaacac
     1261 agcatgatga tgcagtagat aatgaaacaa ttcgtcatga tgtcatcgaa aaagtagtga
     1321 aggaagttat tccagctgaa ttattagatg atcaaacgaa atattatatc aatccgactg
     1381 gccgatttgt cattggtggt cctcaagggg atgccggatt aacaggaaga aaaatcattg
     1441 ttgatacgta tggcggttat gctcgtcatg gtggcggtgc tttttctggt aaagatgcga
     1501 caaaagttga ccgttctgcc agctatgctg cacgttatat tgctaaaaac attgttgcag
     1561 cagggcttgc tcgcaaagca gaagtacagc tagcttatgc gattggtgtt gctcaacctg
     1621 tttcgatctc aatcaatact ttcggtactg gaacggttcc ggaagaagaa ctgattgctg
     1681 cagtgagaga aaacttcgat cttcggccag caggaatcat cgagatgctt gatttgcgtc
     1741 gtccgattta caaacaaaca gctgcttacg gtcattttgg tcgtacagat gtagatttgc
     1801 cttgggaaca tacggacaaa gtagatgcgt tgaaagcaag tttagcaaaa taagttatat
     1861 tcagctggaa cgatcgccaa gtggtggttg ttccagcttt ttttatggca gttaacctct
     1921 tgaaaaagat agtaattatt tatctatttt tagatcctgt cataatgaaa gggaggaata
     1981 agatgaaaaa agaaacaaat gttactttgg tcacagtgag tattttcatt gcaacgttca
     2041 tgacggctat tgaaggtaca atcgtaacaa ccgctatgcc aacgattgtc ggttcattac
     2101 atggaatgga aatcatgaac tgggtatttt cgatttattt gctgacaaac gctatgctta
     2161 ctccgattta cggaaaactt gcggataaag tgggacggaa accaatcttt atttttggga
     2221 cattattgtt tattatcggt tcgttatttt gcggtttatc tcaaagcatg attgggttga
     2281 ttgctgctcg tgcgattcaa gggatgggtg ctggggcaat gatgccagta gctttgacga
     2341 tcatcggcga tctttattct attgaaaagc gagcaaaagt tttaggtttg aatagttcag
     2401 cctgggggat tgccagtgtt tttggaccgc ttgcgggagg attcatcgtt gaaacgatca
     2461 gctggcactg gatctttttt atcaatgtgc cgatcggaat tctattaatt attttgattt
     2521 ggctatattt aaatgaacca aaaatcatac atgagtctaa accaatggat atttttggta
     2581 gttttagttt gatggccatg cttgtctcat tgctgttagg gttccaatta ttaggagacg
     2641 aaggaatgag tccgcttgtt tctgctttgc tgctaggtag tatcgtaatg ttaggtattt
     2701 ttgtttttgc cgaaaaaaga gctaaagatc ctgtcatcat gatgcattta ttcaaaaatc
     2761 cactatttgt tgtggtcaac ttggctgcag cattggctag tggttttttg atgggaatcg
     2821 atgtttatat ccctatgtgg atgcaaggtg tattagggct gaatgcagca attggtgggc
     2881 tggtattagc accattatca ctagtctgga tggtcggttc tttccttgca agcaagtgga
     2941 tgactcaaat gaaaactcat tgggtcttga gaattggact actaatcgca gctattgggg
     3001 gcgcttggtt gtatcttatg cctttcagaa gtaactattt atggtttttt gctatatcaa
     3061 cggtcatcgg tataggctta ggtgtgacca tgactacctc gactgtcagt gcacaaaata
     3121 gtgtatctcg tgaccagctt ggtgtagcaa cttcttttaa tacgttagtt cgaacaattg
     3181 gtcaaacagt catggtttct atttttggtt tattgataaa tggggtaact aaacgagaac
     3241 ttgcagcagc aaatttaaca aatgatgcag atatcatgaa taaactggtg aatccacaaa
     3301 cggctaaata tattgatgcg gatattttgt taccattaag aaaaattctt tatgatgggt
     3361 tacattccgt ttatctagtt ggcttgttac tcgtgatcat tgctttggtc ttaacgcttt
     3421 tcgttcatga aaaaagtggt aaaataaaac agtagtttcg gttaagatat gtacgtagcc
     3481 gaatcattca aatttaaagt tattcaaaaa taacttctta ctaaggaagg cgaaaatatg
     3541 gcatttattc aagcaaatat ttattcgaat gtgttagaaa tggaagtcaa tctaaatgtg
     3601 atactaccgc aaacaacaaa aaaggtgatt ggcaccagtt cagagcaaga gacaacggat
     3661 atacctgttt tatatttact tcatggaatg ggtggcaatc atagtgtatg ggaaagacgt
     3721 acgtcgattg aacgttatgt agcaaactac ggattagctg tcatcatgcc atctactgat
     3781 cttggctggt acacagatac agtttatgac atgaagtatt ggacattcat tgcagaagag
     3841 ctacctgaga tttgtcatga attattcccg caattaacaa gaaaaagaga aaaaacatat
     3901 gccgctggat tatcgatggg cggatatggc gcgttaaaac taggccttgc aaaatcagaa
     3961 aatttcgcag cagtaatctc tctttcaggc gctgtttctg taggaaatcg aatggatgat
     4021 cttttgatgg ttcgtaaagc atctttttgg gaagggatct ttggaccatt ggatcaaatc
     4081 gaaggttcaa aaaatgatcc gcaatactta ttgaaagaac tagtaaaaag cggaaaacca
     4141 gttccaagat tttatatggc ttgtggggaa tctgattttc tttatcaggc aaatcaggaa
     4201 atggtatctg ctatgcagga acaaggcttg gcggtcacct ttgaacaagg acctggcgat
     4261 cataactgga tcttctggga ccaatggatt caacgtgcat tggcgtggct aatggaagaa
     4321 aaataaaaaa agagtcttaa aaagatagct cctatagaga tcacaaacat agtgatctct
     4381 ataggagctt ttttactgct cgttttctct tcttttattt tgttggcata agccttcaat
     4441 ataggtttgt gcactcgtta gaaagagtgc tttttcattc aattctgcat gggactgcat
     4501 actttcttta aatccctcta attgattttc aactacataa ttacgcataa atagaactgt
     4561 ttctttcaaa aattcatttc cgtccataat tttttgtcga agctgctgtt catgctgctg
     4621 atccattaac tgtccaatca ataaattgtg gagagaggca atggcctgat gactttgggt
     4681 gactgaaaat ccttcacgaa caagtaattg gatcatttgc tccaaatgtt ctaatcgact
     4741 cggaaaagcg ggcacagtat tgatttctaa ttctgcccca caagggaaac gtgtataaag
     4801 gtcatagtaa ttttccatat actgcagcaa ctgttcattc caaggcaaag ttggatctgg
     4861 aagcgctaat tcttcttcca ttgtttcagc cattttttgc aagagctgtt gtttgttttt
     4921 gaaataccaa tataaagcag gtgcttgaac atgaattctg tttgcgatgt ttcgcatgga
     4981 aagctgcgaa agagcttgtg tttcagccag gatatcaaag gcggcctgaa tgatgattgc
     5041 ttgagaaagc tttatttcca ttttatcaac ctcgtgtatt gcattatctt tttaaacatg
     5101 gtaaattgtt agtcggtcta attttacagt gttaaattag tcctgtcaat ttaaacaaag
     5161 gggtgtaatc aacgaatgaa attaatctta cgacatgcga aaaaatataa agtagctgtt
     5221 tttatttcct tgctgtccgt agcagtcatg gttgcagcag cattatggca acctaagtta
     5281 ctacagcaag tattggaagc tattattaca gaagataatg atgaaatgaa aacaattggt
     5341 atttacctga tcagtctggc tttgctagga ttagccgcag gtgtaacgaa cacgattttc
     5401 tctgcaaaag tagctcaagg cgtaagtgca gatatccgag aagaagcatt tcgaaagatc
     5461 cagacatttt catttggaaa tatcgaacag ttctctgctg gaaatcttgt tgttcgtcta
     5521 acaaatgata tcacgcaaat ccaaaattta gtgatgattt ctttgcaatc actttttcga
     5581 attccattct tatttattgg tgccttcatt ttagctatga tcacgatgcc gcaattatgg
     5641 tggatcatcg tagcgcttat cgttacggta tttctgatta cggcactttc atttacaagg
     5701 atgggcaagc acttcatgat cattcaaaaa ctgattgatc gtgtgaatag cattgcaaaa
     5761 gaaaacttac tagggattcg tgtagtgaaa tcttttgtac aagaagaaaa ccaattgaag
     5821 tcattcagca aagtaagtga ggacttaaca aaacacaaca tcatagtcgg tacattattt
     5881 tctgtgatga tcccgtcctt catgctggct gccaatttgg ctgtggttgg ggcgatcttt
     5941 tttgtgagcg atcttgttaa agatgatcct acgttaattg gtggaattgc ttcattcatg
     6001 aactacttga tgcagattat gatggcaatc attattggcg gaatgatgat gatgatgact
     6061 tcaagagcag ccgtatcgct gaagcggatt gcggaaattt tagatgctga accagatctt
     6121 acttatttag acgttccaga acaagagttg acgggttctg tcacttttga ccatgtttct
     6181 tttcgttatc ctggtgagga tacagatacg cttaaagata tcagcttttc tatcaaacct
     6241 ggagaaatgg ttggtatcgt cggggcgact ggtgccggga agtcaacact agcacaattg
     6301 atccctcgtc tatttgatcc gacagaaggc aaagttgaag taggtggtgt agatcttcgt
     6361 caagtaaatg aaaagagttt aagacaaacg gtttcctttg ttttgcaaaa agcaatttta
     6421 ttctcaggaa cgattgctca aaacttgcgc caagggaaaa agaatgcaac agaaaaagag
     6481 atggaacatg cttcttctat tgctcaagca aaagaattca tcgaaaagtt atctgatcgt
     6541 tacgaagcac caatagaaga acgtagcagt aacttttctg gtggtcaaaa gcaacgttta
     6601 tccattactc gtggcgttat cggtaatcca aagatcttga tcctagatga cagtacgagt
     6661 gcactggatg ctcgctctga aaagctggtc cgagaagcac tcgatcgaga tttgaaggga
     6721 acaacgacga ttgtcatcgc acaaaaaatt gcttctgttg tcaaagcaga ccgcatctta
     6781 gtattggatg aaggcagatt agtcggcgaa ggaacccatg aggaattggt agctggaaac
     6841 cgtgtctatc aggaaatctt tgaaactcaa aaaggaacgg aggagtaagt atgactgatc
     6901 taataaaagc aagtaaattt ttctatcact atttgaagcg gtacaaactc tcctttttct
     6961 ttatctttat cacagtgatc attgctacct atctacaagt aaaggcacct caatacatcg
     7021 gggaagcttt tcaagaatta gctaattacg taggaggctt gatgcaaggc gtagacgata
     7081 aaagcaaatt tgtttctgta atatggaaac tcttgctgtt ttatgtacta gctagtgcag
     7141 ccaactttat ctatagtatt ttgttcacac aagtagtagg gaagtcaacg aaccgaatgc
     7201 gtatcggtct attcaacaaa ttggaaaaat tgacgatccg tttctttgat tctcatcaag
     7261 acggtgaaat tttaagtcgt tttaccagtg acttagataa tattcaaaat agtttgaacc
     7321 aagctttgct tcaagtcatc acgaatgctg ttttacttgt cgggatcttg atcatgatgt
     7381 tccgccaaaa tgtagagtta gcttgggcta caattgcttc cactccagta gccgttttga
     7441 tagctgtctt gattatccgc aaagcacgga aatatgtgga tcttcagcaa gatgaagtgg
     7501 ggaaactaaa tggttatatg gatgaaaaaa tcagcggaca acgtgtcatc atcacaaatg
     7561 gcttacaaga agaaacgatt gaaggattct tagagcataa tcaaagtgtt cgcgaagcaa
     7621 ccttcaaagg acaagcttat tctggtctac tatttccgat gatgcaagga atgtcactgg
     7681 tcaatacagc gatagttatc tttttcggcg gctggttagc attaaatggt gatttggaac
     7741 gttcggtggc attaggctta gttgtgacat tcgtccagta ttcacaacaa tattaccaac
     7801 cactgatgca gatttcatct ggatacagca tgatccaatt agcgattaca ggggcaagac
     7861 gattgaatga aatatttgat gaaaaagatg aagtcaaacc agaaaacggt aaagcatttg
     7921 atggtgttga aaaaggtctt gcgttgcagc acgtagattt tggttattct ccagaaaaac
     7981 tagtcttgaa agatgtctcg atcaatgtgg acaaaggaga aatggttgct cttgttggtc
     8041 caacaggatc ggggaaaacg acgatcatga atctgctaaa ccgtttttac gatgtcaatg
     8101 atggttcagt agtcatcgat ggaatagata tccgagatta tgatcttgat gcattacgta
     8161 gacatgtagg aatcgttttg caagaatctg tattgttctc cggaacgatc aaagacaata
     8221 tcacattcgg caaaccggac gcttcagatg aagaagtcat caatgcagca aaacaagcga
     8281 atattcatga cttcatcatg acgttagaaa aaggttatga tacagagata tccgaagaaa
     8341 ataacttatt cagtacaggt caaaagcagt tgatcagtat tgctcgaacg atcatcacaa
     8401 atccatcttt attgattttg gatgaagcaa caagtaatgt agacacggta acagaagcaa
     8461 ggatccaaaa agcaatggat gaagcaatca aaggaagaac cagctttgtg attgctcacc
     8521 ggctgaaaac aatcttgaat gcagatagaa tcgttgtctt gcgtgatggg gaagtcattg
     8581 aagaaggcag tcatcatgag ttattagcag aaaatggatt ttatgcggaa ctttatcata
     8641 atcaatttgt atttgaaagt taaattaaaa gaacctcgtt ataacggggt tctttttata
     8701 tattttatgt tgtttgtttt tataaggggc agaa
//
LOCUS       DACGLE010000094         8561 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0534
            SAMEA3304449-rid9560453.denovo.099, whole genome shotgun sequence.
ACCESSION   DACGLE010000094 DACGLE010000000
VERSION     DACGLE010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304449
            Sequence Read Archive: ERR985064
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8561)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8561)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702225.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702225.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 20:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,045
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 27 of 67
            Pseudo Genes (incomplete)         :: 25 of 67
            Pseudo Genes (internal stop)      :: 19 of 67
            Pseudo Genes (multiple problems)  :: 4 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8561
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304449-rid9560453.denovo.099"
                     /strain="VREN0534"
                     /db_xref="taxon:1352"
     gene            complement(138..1091)
                     /gene="menA"
                     /locus_tag="IXT20_002477"
     CDS             complement(138..1091)
                     /gene="menA"
                     /locus_tag="IXT20_002477"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HAQ4860894.1"
                     /translation="MTLKIFLEVVEIRTKVASVFPFAVGVLFSIAFFHEIHWLNTFLF
                     FIGMLVFDLATTAINNYMDYEKAHSEVYKYEENVIGRRNLSPVLIRNMILAMIAFVFV
                     IGVILTINTGWLLLLMGFICCFIGVFYTFGPIPLSRMPLGEIFSGVTMGLGIFAMVVY
                     VNTFGSDVYDLTVNFSSGQFDLVGNLWAIAAIVLASLPLVFTIANIMLANNLRDLDRD
                     IENHRYTLVFYIGRPAGTVLFQLLMYACYFVILMGLLSGIYEWPILVTFLTLPIVKKH
                     LDQFKQTLPQPISFSYSIKNMVLFNASYVLGLLLTIIMQAI"
     gene            complement(1104..2207)
                     /locus_tag="IXT20_002478"
     CDS             complement(1104..2207)
                     /locus_tag="IXT20_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HAQ4860895.1"
                     /translation="MRKKLLFSLITLGISFMVLTGCSNSANKTTTESSAATKILKDPY
                     SDEQFLLGTYVRIRVYDEGKESALKPAFDRVKELGDKITINQKGSEIDEVNEQAGIKP
                     VKVSDDVYTLVKRAYEYSQDSQGGFDMAIGAITQLWRIGFDDARKPSQEEIDQALKLV
                     DYHKIELNDKEKTVYLKEKGMIIDLGAIAKGYITDEVVKVLKKQGVTTAIVDLGGNVY
                     VLGHSPRGENQDWTVGIQDPNMARGSVLGSIKERNKTLVTSGIYERYLEVDGKKYHHL
                     FDPKTGYPFDNDIASVTIITDKSIDGDGLSTAVFSMGVKKGLEYVESELNNGTEAIFV
                     TKDDKVYVTDPIKDTFKLSEDSHYTMGDRSELK"
     gene            complement(2297..3232)
                     /locus_tag="IXT20_002479"
     CDS             complement(2297..3232)
                     /locus_tag="IXT20_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HAQ4860896.1"
                     /translation="MKKKRFVTGFAVLAFSALVLGACGADDNGSSNSSSESSTAQSST
                     AKSTTESSAKVVAGGDLQDGTYKLEEKNYSNGYRAVFEMVVKDGKITESKYDNVNENG
                     ESKTKDAEYNKNMEAKSGTNPEKYIPELNDQFLKAQSASGVEVVTGATHSSESFQNYA
                     QQLIQAAQAGNTDTIEIDNGADLKDGTYKLEEKNYSNGYRVQFEMTVAGGKVTESNFD
                     YIDKDGKSKQDDTEYNENMKAKSGTEPKTYIPTLNDEFVKAMGEEDGSPANVEVVTGA
                     THSSHSFIMYAQQLVNAAEKGDTQTIEVDNIVTEK"
     gene            3534..5468
                     /locus_tag="IXT20_002480"
     CDS             3534..5468
                     /locus_tag="IXT20_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAQ4860897.1"
                     /translation="MAKQNIVVVGAGYAGVSATKYLAKKLKKEDVAITLIDRHSYHTM
                     MTELHEVAGGRVEPEAIQYDLQHLFARQKNVQLVTDTVIGIDKERKIVKTKLGSYPFD
                     QLILGMGGEPNDFGTPGVKAHGFTLWSFEDSVRIREHIEKTVAKAALEPDDELRKAML
                     TFVVCGSGFTGIEMIGELIDWKNRLAKDYKLDPNEITLMVVEAMPTILNMLDRNDAAK
                     AERYLRKHNVELLLNAPIVEVAADHIKLKDGRNIPTHTLIWTAGVKGTSDAADFGLES
                     ARGQRLIANEYMQAKGYEDKNIYIIGDLVFYEEFPGTPTPQIVQAAEQTGHTAAANIV
                     ASIKGGEKHKFKGNYQGFMVSIGAKWGVANLFDKIHLSGFLAIVMKHIVNLKYFFDIR
                     SGYYMFQYLMHEFFHIKDDRNVTRGHSSRYGNVLWSVPLRIFYGLVWFVEASKKIIGN
                     GEVFKPSTWFGEGSWFTGNSVFPWTWEYNVTDVASGASAAGDATTAASGAGASAGEAA
                     TQAAHFGLSYAYGEEPMAVIEKSPDWFTKIMQVIMPNNDVAMFFQKFMVFFEIALALA
                     LIAGLFTWLCSATTIVLVITFCLSGMFYWVNIWFIFVAFALMNGSGRAIGLDRWVIPW
                     LQRTLGNWWYGRPSSQYGKK"
     gene            5925..6341
                     /locus_tag="IXT20_002481"
     CDS             5925..6341
                     /locus_tag="IXT20_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HAQ4860898.1"
                     /translation="MSLKEFAEKSLLKPWDVFIILLLTVLSFLPVMIFGLQQNTATAE
                     KEAVLRVDGTEIKTFPLMAGEESNTYTYKDEHGDYNLIEIDGDRVRIKEADCDDQICV
                     RRGWATKNGETIVCLPHKLVIEVRSTDGGDEDSLIY"
     gene            6355..6921
                     /locus_tag="IXT20_002482"
     CDS             6355..6921
                     /locus_tag="IXT20_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HAQ4860899.1"
                     /translation="MSKLQKMIYISLLVAQGVIIGLLENMIPYPFSFAPGAKLGLANL
                     ITIVAIFTMKKQDSFLLLWLRLFLTTLLGGTISTFLYSMSGALLSYFGMLLVKQLGPK
                     RVSIIGISAAGGFMHNVGQLLMASFIAQSWTVMLYLPVLSVLGILSGIAIGIAANYLL
                     RHVQTLQRFQKDYEQGTKQKWSPMFTKK"
     gene            6944..7930
                     /locus_tag="IXT20_002483"
     CDS             6944..7930
                     /locus_tag="IXT20_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HAQ4860900.1"
                     /translation="MKLHPMWENYPALSKELTETLKRMEETVRLKNKPVEQAIKETIH
                     AGGKLLRPAYQLLFSQFGPEQDRDKAIALAASIEMLHTATLIHDDIVDEADLRRGQPN
                     IRSQFGNVVSVYAGDYLFVCCFKLLSDYSTSLKSLQLNSRSMEKVLNGELGQMDDRYN
                     YELSVKEYLENISGKTAELFQLSCSVGAFESGTSERFAKKAGDIGLSIGMAFQIIDDI
                     LDYTKESQEIGKPVLEDMRQGVYSLPLIYSLQKNKPKLLPYLEKKAAMTEEDVDAVRK
                     IVEHTGGVEEARKLAASYTEKALKEIKKLPATSLRTKENLFSLTQLILDRKD"
     gene            complement(8085..>8561)
                     /gene="scm"
                     /locus_tag="IXT20_002484"
     CDS             complement(8085..>8561)
                     /gene="scm"
                     /locus_tag="IXT20_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HAQ4860901.1"
                     /translation="STDTSASSDSTDTSTTTSDSTDTSASSDSTDTTSTTSDSSDSST
                     TTSDSTDTSASSDSTDTSSTTSDSSDSSTSSDSTDTSSTTSDTRHASVSFSKTSSDNR
                     TNFNLTNKTSNDNGNKSKRALPKTGSQSNNWITLAGVILLVIGLRITFMSYKKSKR"
BASE COUNT         2517 a         1655 c         1737 g         2652 t
ORIGIN      
        1 acctcctgat aaacggtgtt caaaagcttg catctgcggt aataagccca accaaacgaa
       61 aaatataagg agctattttt atttggctgt tcttattact tgatgcaggg cagttttttc
      121 acaacctctt catgctgtta aatggcttgc ataatgattg ttagtaatag ccccaataca
      181 taactggcat tgaataagac catatttttg atggaatagc tgaaactgat aggctggggt
      241 aacgtttgct tgaattgatc caaatgcttc ttcacaattg gcaaggtcaa gaaggtgacc
      301 aagatcggcc attcatagat accgctcaac aaccccatta aaatcacgaa atagcaagca
      361 tacatcaata gctggaataa gactgttccc gcaggacgtc cgatataaaa aactagtgta
      421 tagcgatgat tttcaatatc acggtcaaga tcccgcaaat tattagccaa catgatattg
      481 gcaatcgtaa acaccaaggg taaggaagct aatacgattg ctgctatagc ccaaagattt
      541 cctaccaaat caaattgtcc agatgaaaag ttgactgtca gatcatagac atctgaaccg
      601 aatgtattga cataaacgac catggcaaaa attccaaggc ccatagtcac cccactgaaa
      661 atttccccta aaggcattcg agataaagga atcggtccga atgtatagaa tactccaata
      721 aaacaacaga taaatcccat caaaagtaaa agccaacccg tattgatcgt tagaataacg
      781 ccgataacaa aaacaaatgc aatcatagct aaaatcatgt ttcgaatcaa tacaggagat
      841 agatttctgc ggccaatgac attttcttca tacttatata cttcagaatg agccttttca
      901 taatccatgt aattattgat agcggtagtc gctaaatcaa aaactagcat tccaataaaa
      961 aataaaaaag tgtttagcca atgtatctca tgaaaaaaag cgatagagaa taatacccct
     1021 accgcaaaag gaaatacact agccaccttt gttcgtattt caactacttc taaaaagatt
     1081 tttagtgtca tggttcgtac tccttatttt agttcagaac ggtcacccat cgtgtaatgg
     1141 gagtcctccg ataatttgaa ggtgtctttg attggatcgg tcacatatac tttatcgtct
     1201 tttgtcacga agatcgcttc cgtcccgtta ttcaattcgc tttcgacgta ttccagtcct
     1261 tttttcacac ccattgaaaa gactgctgta gacaacccgt caccatcaat tgatttatct
     1321 gttatgatcg tgacacttgc gatatcattg tcgaacggat aacccgtttt tggatcaaac
     1381 aaatgatggt attttttgcc atctacttct aaataacgct cgtaaattcc agaggtaact
     1441 aatgttttgt tccgttcttt aatagaacct aaaacagatc cgcgagccat gttcggatct
     1501 tggatgccga ctgtccagtc ttggttttcg cctcttgggc tgtggcctaa gacatagaca
     1561 ttaccgccca agtcaacgat ggcagtggtc actccttgtt ttttcaagac tttcactact
     1621 tcgtccgtaa tgtaaccttt ggcgattgca cctaaatcga tgatcattcc tttttctttt
     1681 agataaacag ttttttcttt gtcatttagt tcgatcttat gataatccac taatttcaat
     1741 gcctgatcga tttcttcttg ggaaggtttt ctggcgtcat caaaaccgat tcgccataat
     1801 tgtgtgattg caccaatcgc catatcgaat ccgccttgag aatcttggct atattcgtag
     1861 gcccgcttga ctaacgtgta gacgtcatca gaaactttta ctggtttgat cccagcttgc
     1921 tcattcactt catcgatctc agaacctttt tgattgatcg tgattttatc accaagttct
     1981 tttactcgat cgaaggcagg tttcaatgct gattctttgc cttcatcata cacacggata
     2041 cgaacgtaag tccctaacaa gaactgttca tcagaatatg gatctttcaa tatcttggta
     2101 gctgctgaac tttcagtggt tgttttattt gctgagttgc tgcagccagt tagcaccata
     2161 aaagagatgc ccagtgtgat taaggaaaat agtagttttt tccgcatctt tttcctctct
     2221 tcattttgaa aggtgagacg actgcctaat ctggctatcg tcccaccctc caacttttat
     2281 tagtatgatt atgtgattat ttttctgtta cgatattatc tacttcgatt gtttgagtgt
     2341 cgcctttttc agctgcattc actaattgtt gtgcgtacat gatgaatgaa tgtgagctgt
     2401 gtgtagcacc tgtaacaact tctacattag caggtgagcc atcttcttcg cccattgctt
     2461 tgacgaattc gtcgttcaga gtagggatgt aagtttttgg ttctgtaccg ctttttgctt
     2521 tcatattttc attatattct gtatcatctt gttttgattt accgtctttg tcgatataat
     2581 caaagtttga ttcagttact ttgccgccag caactgtcat ttcgaattgc acacggtagc
     2641 cgttagagta gttcttttct tcaagcttgt aagtgccgtc tttcaaatca gcaccattgt
     2701 cgatttcgat tgtatctgtg ttaccagctt gagcagcttg gatcaattgt tgtgcgtagt
     2761 tttggaagct ttcagatgag tgagtagctc cagttacgac ttccacaccg cttgcgcttt
     2821 gtgcttttaa gaattggtca ttcaactcag gaatgtattt ttctgggttt gtgccagatt
     2881 ttgcttccat gtttttgttg tattctgcat cttttgtttt tgattcgccg ttttcattga
     2941 cgttatcata ttttgattca gtgattttgc cgtcttttac aaccatttca aagactgcac
     3001 ggtaaccatt tgaataattt ttttcttcta atttatatgt gccgtcttgc aaatctccgc
     3061 cagctacaac ttttgcagat gattctgttg tgctttttgc agtcgaagat tgtgctgtag
     3121 atgattcaga agaagagttt gagcttccgt tgtcatctgc gccgcaagcg cctaacacta
     3181 gagctgaaaa tgctaatacg gcaaatccag ttacaaaacg ttttttcttc attttaatac
     3241 cccaccattc catttctagt ccacaatttt tctttgtgat tcattataca ttctaatctt
     3301 tttagcctat aagtcaacta tttttgataa ataacccatg taatatttca aacgttttct
     3361 ttcagcaaat ttcgtgaaaa aacgctttat tttaaaattt gaaaaaaatc caaaaggagt
     3421 tagacaaaag aacagaacat ctatataata tatggagatt gtgtaataag tatcaaacta
     3481 aaagaattat catatattca ttatatagat agtctataag gagcggatga tcaatggcaa
     3541 agcaaaatat tgtcgttgta ggagcaggat atgccggtgt ttcagctaca aaatacttgg
     3601 caaagaaact gaaaaaagaa gatgtagcaa ttactctgat cgatcgtcat tcgtaccata
     3661 ctatgatgac cgagttgcat gaagtagctg gcggacgtgt agaaccagaa gcaatccaat
     3721 acgatctgca gcatttgttt gctcgtcaaa agaatgtaca acttgtcaca gacactgtaa
     3781 tcggtatcga taaagaacga aaaatcgtca agaccaaatt aggttcctat ccttttgatc
     3841 agctgatcct tggaatgggc ggagagccga atgactttgg tacacctggt gtcaaagcac
     3901 acggattcac cctttggtca tttgaagatt ctgtaaggat tcgggaacat attgagaaga
     3961 cagtagcaaa agctgcattg gaaccagatg atgaactgcg taaagccatg ctgactttcg
     4021 ttgtctgtgg ttctggattt accggtattg aaatgattgg ggaactgatc gactggaaaa
     4081 atcgcctagc caaagattat aaattagatc caaacgaaat cacactgatg gtcgtagagg
     4141 caatgccgac gatcttgaat atgttggatc gaaatgatgc tgcaaaagct gaacgctatt
     4201 tgcgtaagca caacgtggaa ttattattga atgcaccaat cgtagaagtg gctgctgatc
     4261 atatcaaatt gaaagacgga cgcaacattc cgactcacac actgatttgg acagctggtg
     4321 tcaaaggaac ttctgacgct gctgactttg gattagaatc agcacgcgga cagcggttga
     4381 tcgccaatga atatatgcag gcaaaaggct acgaagataa aaacatctat atcattggtg
     4441 atttagtctt ttatgaagaa ttcccaggaa caccgacacc gcagatcgtt caagctgctg
     4501 aacaaacagg acacacagcc gcagcgaata tcgtggcttc gatcaaaggc ggagaaaagc
     4561 ataaatttaa aggaaactat caaggattta tggtttctat tggtgccaaa tggggggtag
     4621 ctaacctatt tgataagatc catttaagcg gatttttagc tatcgtaatg aaacatatcg
     4681 tgaatttgaa atacttcttt gatattcgtt ctggttatta tatgttccaa tatttaatgc
     4741 atgagttttt ccatatcaaa gatgatcgca acgtgactcg aggacattct tctcgttacg
     4801 gcaatgtctt atggagtgtg ccacttcgta ttttctatgg tttagtttgg ttcgttgaag
     4861 cttccaaaaa aatcattggg aatggtgaag tgttcaaacc aagtacttgg tttggtgaag
     4921 gatcatggtt cactggaaac agtgtgttcc cttggacatg ggaatacaat gtaacagatg
     4981 ttgccagtgg tgcttctgct gctggtgatg caaccacagc tgccagcgga gcaggggcaa
     5041 gtgccggaga agcagcgact caagctgcac attttggttt gagttatgca tacggtgaag
     5101 aaccaatggc tgtgattgag aagtcaccag actggttcac aaagatcatg caagtgatca
     5161 tgccaaacaa tgatgtagca atgttcttcc aaaaattcat ggtattcttt gaaattgcct
     5221 tggcattagc attgattgct ggattattca catggctatg cagtgcgaca acgattgtat
     5281 tggtgatcac gttctgctta tcaggcatgt tttactgggt aaacatttgg ttcatttttg
     5341 ttgctttcgc attgatgaac ggttccggac gtgcgattgg gttggatcgc tgggtaattc
     5401 catggctaca acgtacacta ggtaattggt ggtacggtag accaagttcc cagtacggca
     5461 agaaataaat agataaagaa cgttatttga atgcaagatt tcaatagaaa ggggctgaga
     5521 caaaccgtca cagcctcctt tttatgtaaa attaaaggaa taagatgccc gagataagcc
     5581 tccatgagca agacacaggt gtcacaacct ctgttacaac ctcttgttat aaacggtgtt
     5641 ccagaagtag cttcttcgaa aaataagccg aaatttcaca aaaacttgaa aagtaatttt
     5701 cgtaaattcc ttcttatttc ttgaagttgc ccacttctgt cacaacctct tgttataaac
     5761 ggtgttccac ctgctgcttc tcggtaataa gcccaaagaa ttagaaaaca tggaaagctg
     5821 ttttctattc ttcgttctta ttactcaaag caagacacag gtgtcacaac ctcttgtttt
     5881 tatgctaaaa ttgggtgagt taatctggaa aaaggagaac aaaaatgagc cttaaagaat
     5941 ttgctgaaaa aagtctgctc aaaccttggg atgtttttat tattttgctt ttgactgttc
     6001 tttctttttt accagtcatg atttttggtc ttcagcaaaa caccgcaaca gcagaaaaag
     6061 aagcggtact gcgtgtggat ggcacagaaa tcaaaacatt ccctttgatg gctggagaag
     6121 agagcaacac atatacttac aaagatgaac acggagatta taacttgatc gagatagatg
     6181 gcgatcgagt acggataaaa gaagcagatt gcgatgacca gatctgcgta cgtcgaggct
     6241 gggcaacgaa aaatggcgaa accatcgtct gtttgccaca taaattagtg atcgaagtcc
     6301 gttcaacaga tggaggtgac gaagacagtc tgatttatta gaccttcaca caccatgagt
     6361 aaattacaaa aaatgattta tatttcgcta cttgttgcac aaggagtgat cattggttta
     6421 ttggaaaata tgatccctta tcctttttct tttgcgcctg gtgcaaaact aggcttagcc
     6481 aatctaatca cgatcgttgc catttttacc atgaaaaaac aagacagctt cttactactt
     6541 tggctacgac tattcctgac tactttgcta ggcggaacga tttccacatt cctttatagt
     6601 atgagtggtg ctttgcttag ttattttgga atgttattgg tcaagcaatt aggaccaaaa
     6661 cgtgtcagta tcatcggaat tagtgcggcc ggcggtttca tgcataatgt cggacagctt
     6721 ttgatggctt ctttcattgc acaatcatgg acagtcatgt tgtacttgcc agtcctttca
     6781 gttttaggga ttttatcagg gattgcaatc gggatcgcag cgaattatct tttacgacat
     6841 gtgcagacac ttcaacgatt ccaaaaagat tatgagcagg gaaccaaaca aaaatggagc
     6901 ccaatgttta ccaaaaaata atcgtcacac aaggagtttt tttatgaagc tacatccaat
     6961 gtgggaaaac tatcctgctt taagtaaaga attgacagaa acactaaaac ggatggaaga
     7021 aactgtccgc ctgaaaaaca aacctgtcga acaagccatc aaagaaacga tccatgccgg
     7081 cggaaagctg ttgcgtcctg cctatcagct attgttctca caatttggac cagagcaaga
     7141 tcgagacaaa gccatcgctt tggcagcttc tatcgaaatg ctccatacgg ccacactgat
     7201 ccatgatgat atcgttgatg aagcggatct gcgacgtggt cagcctaata tccgtagtca
     7261 gtttggtaat gtcgtttctg tctatgcagg agactattta tttgtctgct gttttaaact
     7321 attgtctgat tactccactt ccttgaaaag tctccaattg aattctcgca gtatggaaaa
     7381 agtattgaat ggcgaattag ggcagatgga tgatcgctat aattatgaac tttctgtaaa
     7441 agaatatttg gaaaatattt caggtaagac agctgaactg ttccaattaa gttgttctgt
     7501 tggcgctttt gaaagtggaa caagcgagcg cttcgcaaaa aaagcaggag atatcggact
     7561 ttctatcggg atggcctttc agattatcga tgatatttta gattatacaa aagagagcca
     7621 ggaaatcggc aaacctgtac tagaagacat gcgtcaaggg gtctattctt taccgttgat
     7681 ctactctttg caaaaaaaca aaccaaagct actaccttat ttagagaaga aagcggcaat
     7741 gacagaagaa gatgtcgacg ccgtacgcaa aatcgttgaa catacaggag gcgtggaaga
     7801 agctaggaag ttagcggcct cttacacaga aaaggctttg aaggaaataa agaaattacc
     7861 tgccacctct cttcgaacaa aagagaatct cttttccctt acccaactga ttttggatcg
     7921 caaagactga atagggaaag gttcgatacg taaataaatt tctcagaagg aaatctacgt
     7981 gttttttgac aaattgaatt gtagttcttg tatttatgat aaaaagcaaa aataaagagc
     8041 aacgtccgtt agcgacgttg ctctttttta tcttgaatat ttttttacct tttcgatttc
     8101 ttataactca taaaagtgat tcttaaaccg attacaagta atataacgcc tgcgagtgtt
     8161 atccaattat tcgattgact tccagtttta ggaagtgctc tttttgattt gtttccattg
     8221 tcattgcttg ttttattagt taagttaaag ttcgtcctgt tatccgaact tgtttttgag
     8281 aaggagacac ttgcatgacg tgtatcgctt gtggtagacg acgtgtctgt actatcgctt
     8341 gaagttgatg aatctgagct atcacttgtg gtagaggatg tatctgtact gtcacttgaa
     8401 gctgacgtgt ccgtgctatc acttgtagtg gtagatgaat ctgagctatc acttgtggta
     8461 gaggtagtat ctgtactgtc acttgaagct gacgtgtccg tgctatcact tgttgtggta
     8521 gatgtatccg tactgtcgct tgaagctgac gtgtccgtgc t
//
LOCUS       DACGLE010000095         8485 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0534
            SAMEA3304449-rid9560453.denovo.100, whole genome shotgun sequence.
ACCESSION   DACGLE010000095 DACGLE010000000
VERSION     DACGLE010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304449
            Sequence Read Archive: ERR985064
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8485)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8485)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702225.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702225.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 20:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,045
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 27 of 67
            Pseudo Genes (incomplete)         :: 25 of 67
            Pseudo Genes (internal stop)      :: 19 of 67
            Pseudo Genes (multiple problems)  :: 4 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8485
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304449-rid9560453.denovo.100"
                     /strain="VREN0534"
                     /db_xref="taxon:1352"
     gene            complement(22..879)
                     /locus_tag="IXT20_002485"
     CDS             complement(22..879)
                     /locus_tag="IXT20_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAQ4860902.1"
                     /translation="MYGGIEAGGTKFVCAVSDGGEILEKLSIPTTTPEETLDQVFEFF
                     DRYELKGIGIGSFGPIGIDKTKDNYGYVLATPKKGWAQFDFLGTIKERYNIPAAWTTD
                     VNAAAYGELKQGAAQGKNSCIYLTVGTGIGAGVVINEEIFSGIAHPEMGHIWVKRHPD
                     DHYEGTCPYHKDCLEGLAAGPSIEARTGIKGQDLPQDHPVWAIQAYYIAQALINYTLT
                     LAPEKIILGGGVMNQDHLLQKIRQQFVELMGGYMETPQVSEYIVRWGLPNESGIIGSL
                     LLAEHEHQK"
     gene            complement(864..3566)
                     /locus_tag="IXT20_002486"
     CDS             complement(864..3566)
                     /locus_tag="IXT20_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HAQ4860903.1"
                     /translation="MKKKVYIISHSHWDREWYMAYEQHHMRLIELIDDLLELFEVDPS
                     FNSFHLDGQTIILDDYLQVRPEKRQAIQQAINEGKLRIGPFYILQDDFLISSESNVRN
                     MLIGMEESRKWGTPVMLGYFPDTFGNMGQTPQLMKQAGISAAAFGRGVKPIGFDNQVL
                     EAENYSSQYSEMWWKGPDQTAIFGLLFANWYSNGNEIPVEKEAALAFWKQKLADAEQY
                     ASTNHLLMMNGVDHQPVQKDISKAIHLANELFPDYEFIHSNFTDYLEAVQKDVPEDLG
                     SVEGELTSQETDGWYTLANTASARVYLKQWNTKVQRQLENITEPLATMAYEVSGNYPH
                     DQLDYAWKTLMQNHPHDSICGCSVDSVHREMIPRFEKADEVGKYLAQDSLEQLTAAID
                     TTGFPKDSFPFVIVNTAGMDKTGEAEITIELERKRFAEGIPEQLYQELENLPKRKYHV
                     ETKSGATIPAILSEETVQFGYDLPKDRFRVPYMARMIKVTLPLENMPAFSWETFALVE
                     GEAKAEEKETMIHQSGRIIENGPLHLTIEKNGTITMEDRKNKRKLNNLHIFEDIGDIG
                     NEYIFKQPFCDQPILSSNKENSEVKVLVDTPEIAKISILQEMEIPVSADERLEKEQQM
                     VVEFRYRKAERSKEKRILQIKTIMTIRKDSKKIDFETTLDNQMKDHRLRVLFPTKLHV
                     EHHEADSIFEVVKRPNHVSKSWENPTNPQHQQAFVNIHDEEYGVTVGNFGLNEYEVTE
                     DGQIAVTLLRSVGELGDWGYFPTPEAQCLGEHRFNYSIELHGPEEKFSTYLHAYAAQI
                     PFSTQQIKHHEGTLISKQQYLTIKSETFAITALKRSKFSDKVVVRGFNMSSHLEKLEI
                     TKDNGKTVILNLLEEPTKQAVVPIIQPYEIRTIGFEEENECMAE"
     gene            complement(3579..4868)
                     /locus_tag="IXT20_002487"
     CDS             complement(3579..4868)
                     /locus_tag="IXT20_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 125 protein"
                     /protein_id="HAQ4860904.1"
                     /translation="MAYKDIPKSVQKFMEDITQKCGDIHADWAKNFTAAFANTLLTTV
                     KKQNDGTTFLLTGDIPAMWLRDSTAQVRPYLVIAKEDEELADMISGLVKKQFFYINID
                     PYANAFNEEANGAGHQTDHTKMNDWIWERKYEIDSLCYPIQLAYLLYKNTGKTDQFNQ
                     SFIEGIKKILDVFKTEQDHEHSPYTFVRDTTRKEDTLINDGRGAKTAFTGMTWSGFRP
                     SDDTCRYGYLVPSNMFAVVVLGYVEEIFQTILDDKEIVQQTQKLKKEIQEGIQNHGRT
                     VNQKGQTIYAYEVDGMGNATVMDDSNVPNLIAAPYLGYCSEQEEDYLQTRRTLLSKEN
                     PYFYEGKYAKGIGSSHTPEKYVWPIALAMEGMTTSDKSEKERILNLLVATDAGTHLMH
                     EGFDVNNPENYTREWFSWANMMFCELVMDYFDIRVEK"
     gene            5018..6064
                     /locus_tag="IXT20_002488"
     CDS             5018..6064
                     /locus_tag="IXT20_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ4860905.1"
                     /translation="MNQPLYQTIFNDLQTAILTGELPVDSQLPTEKELSRTYKVSRIT
                     SKRALTELEQLGLIYRVRGKGSFVKSASPEQILASPTKTRRILFLLPYVADLSVGDFS
                     KGLNPVMQDKQIDVMMTTLDFLQNKTAKELINEFDGLIYYALDTEQHLDLLFELSLKE
                     FPVVILDKKIYELPFPTVLSDNFQGGALAASYLIEQGHSKIAYLFGTQTHPQSVRQRY
                     LGYLEALDKAGLTFHTSLDDKSATLDELLSYITKYEVTAFVCENDVVAIQTMNLLRQH
                     GYHIPEDFSIIGFDDIQAAALVDPPLTTIAQDFEQLGKLAGDALVTWLETKDRPSDSK
                     FPVSFIKRQSTKEK"
     gene            6066..8219
                     /locus_tag="IXT20_002489"
     CDS             6066..8219
                     /locus_tag="IXT20_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HAQ4860906.1"
                     /translation="MNIGQIDTRHATANQPNFSNGNCQPYTGVPFGMNYFAPQTTDQN
                     GSWWFHPDDRVFQGYRLTHQPSPWMGDFSHMLFTPINGKLQENTLFHAQSSYRPEESI
                     FCPTHLSICQLRYGICSTLIPSMYGGILSIDYSKNDSGLLLSFPGRHQLFVIDPYTVE
                     GKITHFAGSEDPDFSFYFILRFEYPLTIDELAVSNKNSDSIPLYFEQTKKQTIRFGTS
                     FISSEQAAYNLELEQNQSKETYLAHSQEQWQSYFDRIQLTHHDQQQLRTFYHNLYRVF
                     LFPQTFYEIDPSGQKIHYDTTSRSIKPGVLYTNNGFWDTYKTVYPLYSLIAVEKYEEM
                     LEGFLNSYRESGYLPKWLSPDERGLMPGTLIDAVIADAAVKDIRRDLMPEFLTAMEKG
                     ATVQSKHPNYGRQGTEDYLTYGYVPNHYHESVNHTLDYAYSDFCISQVAKVLEEEEMT
                     KKYQQQSLNYQNIFDYESGFMRSKDKDGKFREPFSANRWGQDYAEGSAWQSSFAVYHD
                     FSGLIGKYGGSERYKEKLIELCNQPPTFDPGGYGFEIHEMSEMAAIEFGQLAISNQPS
                     FHYPYLFSYIGQPEMAQPLLKQLMTQTFDDSPMGYPGDEDNGSMAAWYIFNSLGFYPV
                     TPGTGEYVIGMPLIDKAVLLLSNGKELTIEATPNRPQQQFIHKIKRMDKEYTKLYFTH
                     QDLLKGGTISYQLGIIPHPKKYQAKDLPFSLSNYN"
BASE COUNT         2370 a         1763 c         1544 g         2808 t
ORIGIN      
        1 ctttttgtgt aatttgtctg cttacttttg atgttcgtgt tctgctaaca gcaaactacc
       61 gataatccca ctttcattcg gcagtcccca acgaacgata tattcactta cttgaggtgt
      121 ttccatataa ccacccatca attcaacaaa ttgctgacgg attttttgaa ggagatgatc
      181 ctgattcatt accccaccgc ctaaaataat cttttcaggt gctaacgtca acgtatagtt
      241 gatcagtgct tgagcaatat aataagcctg aatagcccaa acaggatgat cttgcggcag
      301 atcttgccct ttgatccctg tgcgtgcctc aatagacggt cctgctgcta atccttctaa
      361 acaatcttta tgataaggac aagtaccttc ataatggtca tctggatggc gtttcaccca
      421 aatatgtccc atttcaggat gtgcgatccc tgaaaaaatt tcttcattga tcacgacacc
      481 tgcaccgatt cctgttccca cggtaaggta aatacaacta ttttttcctt gtgctgctcc
      541 ttgcttcaat tccccatatg cagcagcatt gacatccgtt gtccaagcag caggaatatt
      601 gtagcgttcc ttgattgtgc caagaaaatc gaattgtgcc cacccttttt tgggagttgc
      661 caaaacgtat ccgtaattat cttttgtttt gtcgatacca atcgggccga atgagccaat
      721 cccgattccc ttcaattcat aacgatcaaa aaattcaaat acctgatcca atgtttcttc
      781 tggtgttgtg gtagggatac ttaatttttc taaaatttct ccaccatctg aaaccgcaca
      841 cacaaatttt gttccgcctg cttctattcc gccatacatt cgttttcctc ctcgaatccg
      901 attgtccgaa tctcataagg ttggattatt gggacaaccg cttgcttcgt tggttcttca
      961 agcagattca atatgacagt ttttccattg tctttagtta tttccaattt ttctaaatga
     1021 ctagacatat tgaaacctct tactactact ttatcactaa acttgctgcg tttcaaagct
     1081 gtaattgcaa acgtttcact ttttatcgtt aaatattgtt gttttgaaat caatgttcct
     1141 tcatgatgtt tgatttgttg agtggaaaaa ggaatctgtg ctgcgtatgc atgcaaataa
     1201 gtagagaatt tttcttctgg tccatgtagt tcaatgctgt aattgaaccg atgctcacct
     1261 agacactgtg cttctggtgt tggaaaatat ccccaatcgc ctagttctcc cacactgcga
     1321 agtagcgtca ctgcgatttg tccgtcttct gttacttcat attcattcaa accaaagttt
     1381 ccgactgtta caccgtactc ttcatcatga atattgacaa atgcttgctg atgttgcgga
     1441 ttcgtggggt tttcccaact tttagacaca tggtttgggc gcttcaccac ttcaaaaata
     1501 ctatctgctt cgtgatgttc cacatgtaac tttgtaggaa acaagacacg taagcgatga
     1561 tctttcatct gattgtcgag agtcgtttca aagtcgattt ttttactatc ttttctgatt
     1621 gtcatgattg tttttatttg aaggattcgt ttttctttgg atcgttctgc ttttcgataa
     1681 cgaaattcca caaccatctg ctgttctttt tctaaacgct cgtctgcaga aacagggatt
     1741 tccatttcct gtagaatact tatttttgct atttccggtg tatctactaa tacttttact
     1801 tcagagtttt ctttgttact ggataggatt ggctgatcac agaaaggttg tttaaagata
     1861 tactcatttc ctatatcgcc gatatcttca aaaatatgca gattattcaa ctttcgtttg
     1921 tttttgcgat cttccattgt aatggtacca tttttttcaa tcgtcaaatg tagagggccg
     1981 ttttcgatga ttcgtccact ctgatgaatc attgtttcct tttcttctgc tttcgcttcc
     2041 ccttctacaa gcgcgaatgt ttcccaagaa aatgctggca tgttttctaa tggtaaagtc
     2101 actttgatca ttcttgccat atatggcaca cggaaacggt ctttcggcaa atcataacca
     2161 aattggacag tttcttcaga taaaatagct ggtatagtgg ccccactttt tgtttctaca
     2221 tgatacttac gtttaggcaa attttctaac tcttgatata gttgttcagg tattccttca
     2281 gcgaatcttt ttctttctaa ttcgatggtt atttctgctt caccagtttt atccattcct
     2341 gcagtattta caatgacaaa tggaaagctg tcttttggga atccggtcgt atcgattgct
     2401 gcggtcagct gttctaagga atcttgtgca agatactttc caacttcatc tgctttttca
     2461 aaacgaggta tcatttcccg atggacagaa tcgactgaac aaccgcaaat actgtcgtga
     2521 ggatgatttt gcattaatgt cttccaagca taatctaatt gatcatgggg atagtttcca
     2581 ctaacttcat aagccatcgt cgcaagtggt tctgtaatat tttctagctg tcgctggacc
     2641 ttggtattcc attgttttaa gtaaacccga gcagatgctg tattggctaa agtataccag
     2701 ccatccgttt cttgacttgt caattctcct tctactgagc ctaagtcttc tggcacatct
     2761 ttttgaactg cttccagata gtcagtaaaa ttagaatgaa taaactcgta atctggaaac
     2821 agttcatttg cgagatggat tgcttttgat atatcttttt gtaccggttg atgatcaaca
     2881 ccattcatca tcaataagtg gttcgtagag gcatattgtt ctgcatccgc taatttttgc
     2941 ttccaaaagg caagagctgc ttctttttct actggaatct cattcccgtt gctataccaa
     3001 ttggcaaata gcagaccaaa gattgctgtt tgatcaggtc ctttccacca catctctgag
     3061 tattgggaag aatagttttc tgcttccaaa acttgattat caaagccaat tggttttaca
     3121 ccacggccaa acgctgctgc tgaaatacca gcttgtttca ttaattgagg cgtctgtccc
     3181 atatttccaa atgtatcggg aaaatagcct aacatgacag gggttcccca tttacggctt
     3241 tcttccatgc caatcagcat atttctcacg ttcgattccg agctgataag aaaatcatct
     3301 tgcaagatat aaaatggtcc aattctcaat tttccttcat tgattgcttg ttggattgcc
     3361 tgtctttttt ctggccttac ttgaagatag tcatctaaaa taatcgtttg cccatccaaa
     3421 tgaaaactgt tgaaggacgg atcaacttca aatagctcta gtagatcgtc tatcaattca
     3481 atcagtcgca tgtgatgctg ttcgtaagcc atgtaccatt cacgatccca gtggctatga
     3541 gaaatgatat atactttctt tttcatttac gatacctcct acttttctac tctaatatca
     3601 aagtaatcca tgactaattc gcaaaacatc atatttgccc aagaaaacca ttctctcgta
     3661 taattttcag gattgttcac atcgaatcct tcgtgcataa ggtgtgttcc tgcgtcagtt
     3721 gctacaagta aattcaatat ccgctctttt tcagatttgt ctgacgttgt cattccttcc
     3781 atagctaatg caatcggcca aacgtatttt tctggcgtat gggaggatcc aattcctttt
     3841 gcgtactttc cttcatagaa atatggattt tctttactca acaatgttct tcttgtttgc
     3901 agataatctt cctcttgttc agaacaatat cccaagtaag gtgcggcgat cagattgggc
     3961 acattactgt catccatgac tgtggcgttt cccattccat ctacttcata tgcataaatc
     4021 gtttgccctt tttgattaac cgttcgtccg tgattctgaa tcccctcttg gatctctttc
     4081 ttcaatttct gtgtttgctg tacaatttct ttatcatcga gtatcgtttg aaaaatttct
     4141 tccacataac ctaacacgac caccgcaaac atattggatg gtaccagata tccataacgg
     4201 caagtatcat cgctaggacg gaaaccagac catgtcattc ctgtaaaagc tgtttttgca
     4261 cctcgcccgt cattgatcag cgtatcttct tttcttgtcg tgtctcgtac aaatgtataa
     4321 ggagagtgtt catgatcttg ctctgttttg aacacgtcca agattttttt gataccttca
     4381 ataaaagatt gattgaattg atcagttttt cctgtatttt tgtaaagaag ataggccaac
     4441 tgtattgggt agcataaaga atcaatttca tattttcttt cccagatcca atcgttcatt
     4501 tttgtatgat ctgtttggtg gccagcaccg ttagcttctt cgttgaaggc attcgcatat
     4561 ggatcgatat tgatataaaa aaattgcttt ttgactaacc cgctgatcat gtcagctaat
     4621 tcctcatctt cttttgcaat cactaaataa ggacgaactt gtgcagtaga gtctcttaac
     4681 cacatcgctg gaatgtctcc cgtcaataaa aaggtggttc cgtcattttg ctttttgact
     4741 gttgtcaaca gagtattggc aaatgctgct gtaaaattct ttgcccagtc cgcatggata
     4801 tccccacatt tttgtgtgat atcttccata aatttctgca ccgattttgg tatatcttta
     4861 taagccacta ataaaacctt ctttctattt gatatatctt taaagattat acttcataca
     4921 gccaattatg tctacgcttt caaaaaatat ctttttattt actttttcat ccattatggt
     4981 attatttgaa tatgatatat cattaagagg tgtttatatg aatcaaccat tgtaccagac
     5041 aatatttaat gatttgcaaa cggctatttt aactggtgaa ttacctgtag acagccaact
     5101 accaactgaa aaagaacttt cacgtaccta taaagtaagc agaatcactt ccaaacgtgc
     5161 attgactgaa ttagagcaat taggacttat ttatcgcgtt cgcgggaaag gcagtttcgt
     5221 taaatccgct tctccggaac agattcttgc ttctcctaca aaaacacgac ggattctgtt
     5281 tcttcttcca tatgtcgctg atttgtctgt tggtgatttt agcaagggat tgaaccctgt
     5341 gatgcaagat aaacagattg atgtcatgat gacgacgctt gactttctac aaaataaaac
     5401 agcaaaagag ctaatcaatg aatttgacgg attgatttat tatgctcttg atacagaaca
     5461 acatcttgac ttactttttg agctttcatt gaaagaattc cctgtcgtca tattagataa
     5521 aaaaatctat gagttacctt ttcctaccgt tctttccgat aatttccaag gcggtgcgct
     5581 tgctgcctct tatttgatcg aacagggaca ttcaaaaatc gcttatttgt ttggtacgca
     5641 gactcatcca caatccgttc gacagcgtta ccttggttac cttgaagcat tggacaaagc
     5701 tggtttgact ttccacactt cactggatga caaaagcgcg acattagatg aattgctttc
     5761 ttatatcact aagtatgaag taactgcttt tgtttgtgaa aacgatgttg tcgctatcca
     5821 gaccatgaat ctcttgagac agcatggtta tcatatccct gaagattttt caattatcgg
     5881 atttgatgat atccaagcag cagctcttgt tgacccgcca ctgacaacca tcgcacaaga
     5941 ttttgaacag ttaggtaagc ttgcgggaga tgctttggtc acttggttag agacaaaaga
     6001 tcgaccatcc gatagtaaat ttccagtttc attcatcaaa agacaatcaa caaaggagaa
     6061 ataaaatgaa tatcggtcaa atagatacta gacatgcaac agcgaatcag ccaaatttct
     6121 ctaatgggaa ctgccagcct tatactggcg ttccttttgg gatgaattat tttgcaccgc
     6181 aaactacgga tcaaaacggc agctggtggt ttcatcctga tgaccgcgtt tttcaaggct
     6241 accgtcttac ccatcaacca agtccttgga tgggtgattt cagtcatatg ctgtttacgc
     6301 cgatcaatgg aaaactacag gagaacaccc ttttccatgc tcaaagttct taccgcccag
     6361 aagaaagtat attttgcccg actcatctgt cgatctgtca attacgttat ggcatctgtt
     6421 ctacattgat acctagcatg tatggcggaa tcttatctat tgattacagc aaaaatgaca
     6481 gcggtttact tttatccttt ccgggacgcc atcagctctt tgttatagat ccttacacag
     6541 tggaaggaaa aatcacacat tttgctggtt cagaagatcc tgatttttct ttttatttta
     6601 tattgagatt tgaatatcct ttaacaattg atgagttagc tgtttccaac aaaaatagcg
     6661 actcgatccc gttgtatttt gaacagacaa aaaaacaaac tatccgattt gggacatctt
     6721 ttatcagctc ggaacaagca gcttataacc tggaactaga acaaaaccaa agcaaagaga
     6781 cttaccttgc tcacagtcaa gaacaatggc agtcctattt tgatcgtata cagctcacac
     6841 accatgatca acaacaattg cggacgtttt atcataattt atatcgggtg tttttatttc
     6901 cacaaacatt ttatgaaatt gatcctagtg ggcaaaaaat ccactatgat acgactagtc
     6961 gatctataaa acctggtgtt ctctatacaa ataatgggtt ttgggatacg tataaaacgg
     7021 tttatcctct ttattctttg attgctgtag aaaaatacga agaaatgctg gaaggttttt
     7081 tgaacagcta tagagaaagt ggctatttgc caaaatggct gtctcctgat gaacgtggac
     7141 ttatgcctgg gacattgatc gatgcggtca ttgcagatgc tgctgtaaaa gatatccgac
     7201 gagacttgat gcctgaattt ctaacggcaa tggaaaaagg tgctacggtt caaagcaaac
     7261 atccaaatta tggacgccaa ggaacagaag attacttgac atatggctat gttcctaatc
     7321 actaccacga atcagtcaat cacacattag actatgctta tagcgatttt tgtattagcc
     7381 aagttgccaa ggtactagaa gaagaggaaa tgacgaagaa atatcagcag caatcattga
     7441 actatcaaaa tatctttgac tatgagtcag gatttatgcg ttcaaaagat aaagatggca
     7501 agttcagaga accattttca gctaatcgtt ggggacaaga ttatgcagaa ggaagtgctt
     7561 ggcaaagcag ctttgctgtt tatcacgatt tttcaggact gatcggaaaa tatggtggca
     7621 gtgagagata taaagaaaaa ctcattgagc tgtgcaatca gcccccaacg tttgacccag
     7681 gaggatatgg ttttgagatc cacgaaatga gtgagatggc cgcgatagaa tttggacaat
     7741 tagctatttc aaaccagcca agttttcact atccttactt gttcagttat ataggacaac
     7801 ctgaaatggc acagccactg cttaaacagt tgatgactca gaccttcgac gattctccaa
     7861 tgggttaccc tggagatgag gacaatggaa gtatggccgc ttggtatatc ttcaatagtc
     7921 tgggatttta tcctgtcacg cctggaaccg gtgaatatgt tatcggtatg ccattaattg
     7981 acaaggctgt tttgcttttg tcaaatggaa aggaattgac gattgaagca acgcctaatc
     8041 ggccgcagca acaatttatc cataaaatca aacggatgga caaagaatat acgaaattat
     8101 attttaccca tcaagatttg ctgaaaggcg gcacgatctc ttaccaatta gggatcatcc
     8161 ctcaccctaa gaaatatcaa gcaaaagatt tgcctttttc cttatcaaac tataattaac
     8221 aagaggttgt gaaaaagctg tcctgcatca agtaataaga acagccaagc aaaaatagat
     8281 cctcatattt ttcgtttggt tggtcttatt accgcagatg caagcttttg aacaccgttt
     8341 attgagaggt tgtgaaatca gcgttctgac ctgagtatta gagagacaaa tggcaaaata
     8401 gttcctcata ttttgccatt ttttgagcta ataccgaggg tcagctgatt gaacaccgtt
     8461 tagtggagag gtcgtgaaaa agctg
//
LOCUS       DACGLE010000096         8379 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0534
            SAMEA3304449-rid9560453.denovo.101, whole genome shotgun sequence.
ACCESSION   DACGLE010000096 DACGLE010000000
VERSION     DACGLE010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304449
            Sequence Read Archive: ERR985064
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8379)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8379)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702225.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702225.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 20:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,045
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 27 of 67
            Pseudo Genes (incomplete)         :: 25 of 67
            Pseudo Genes (internal stop)      :: 19 of 67
            Pseudo Genes (multiple problems)  :: 4 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8379
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304449-rid9560453.denovo.101"
                     /strain="VREN0534"
                     /db_xref="taxon:1352"
     gene            <1..180
                     /locus_tag="IXT20_002490"
     CDS             <1..180
                     /locus_tag="IXT20_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4860907.1"
                     /translation="TKKMMETIGPALSAQRAVMQNIDTEEFRRATERMKQSLSPKQAL
                     KSDYKIADDIEDSNK"
     gene            285..1196
                     /locus_tag="IXT20_002491"
     CDS             285..1196
                     /locus_tag="IXT20_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4860908.1"
                     /translation="MGERTFEDVKDYVELQSQEKCTVLSAKPEQEFNDLGTEVTVWNV
                     KTDTDGDWWVVEGDNVPMNLYPQSAYYFGTDEVYSFHMGLMERMRVLHEKYDPEDFVR
                     SVTLDTEIAPQLFRKLKNVATLIDSAEEIEDFQSIGVQCREILIELGNHIFIADMAGT
                     KEQPQKSNFKKKAELFIQFYLTGSDNSDYRSIIKKLTESTWDYANKLTHSSTTTKYEA
                     STCVMLVTSLVGVYENITQKVFDPVSQYICSSCKSKKLTIDSDDSDENGMVESLYLKC
                     EECGDITKVVFLTSDKDSSYTKGKIIE"
     gene            complement(1530..2672)
                     /locus_tag="IXT20_002492"
     CDS             complement(1530..2672)
                     /locus_tag="IXT20_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAQ4860909.1"
                     /translation="MAKISKYKKKNGQVAWMYKGHIATDPRTGEKINTTRRGFSSKQE
                     AQKDYDEYRHQILYGVKKKAPDMTFEDLYNEWITHQRTSVKASTIAISVRYANNQILP
                     AFGKLKLSNISVPYCQKVVDEWHSKYESYDYMRKQTAQILRYGVAMQYIDNNPMEKTL
                     LPRKKEYEKNRKFYSKEELNNLLDAFKDFGNMKQYAFFRLLAYTGMRKSEVLALQWKD
                     IDTFNKELHVNKTLAVDEFGKVIIQSPKTRASRRVISLDTETLSILNNWKLQQKEEYL
                     KLGYNTSSKEQHVFTTVKNTLYIPNTVNDWLRYILKKYNLPRITPHGFRHTHASLLLE
                     AGESVKVVQQRLGHENSKVTLDIYAHITNNAPKKTGQDFADMMAHQ"
     gene            complement(2711..3445)
                     /locus_tag="IXT20_002493"
     CDS             complement(2711..3445)
                     /locus_tag="IXT20_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ4860910.1"
                     /translation="MIKRPPINYLERKKILGTKIKAIRKSKKLTQPAFGLMINNGQLI
                     DKKTIYEWEKGTYLPIPERLSRIADLGNMSIEELVCGNVEEYILGIILYRDSIVLDGI
                     TFPDKNLFQHLRQQFPPVHSNLDTWLDRYSKLEPEMQEFIANKTCNKVKNEKISLFNI
                     LKIEELFINAIVEEFDNNILFLTSSIEELLERMVDEWLPIQLKDMSYPEEAVREITDN
                     INKLEQTISSIGKKYTKKKMKGGDTI"
     gene            3677..3952
                     /locus_tag="IXT20_002494"
     CDS             3677..3952
                     /locus_tag="IXT20_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ4860911.1"
                     /translation="MSLQIIDSIVSIQVEQQVKEIITVLKPMLEDIISGVTLEYMDYQ
                     QASEYLGVSVGTIRKYVSQYGLPVIKIDTVIRFKKSDIDSFMERYKG"
     gene            4050..6047
                     /locus_tag="IXT20_002495"
     CDS             4050..6047
                     /locus_tag="IXT20_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4860912.1"
                     /translation="METSMINIPIYVDQEKLLEKPVTKKEKTDVRDRCLDNYREVTRD
                     EFVEILNSSQSFIPSKTKNKGNDKESFVETRVIILDVDNTVKDEKGKVVDLSKDDSRY
                     LSIEKVLSINLVHNSAFAIQKSIRYSENIEKYKIVFLLKEAITDYGKMVAVYEYLKKN
                     IPGCDDNVNASNRMFFGGYKSDAVIIINEDNMLDITELPIDFTMTCSKNCQSDSGREY
                     ELVNETNFVKLIKNDDKEEMKQWFKDCLFDSSDMSFNEIYERLLTIDMNKLLKSSKNL
                     RCLFHNDHNPSASIFTSKTGHSIFYCHSSNCDISENFIGVVMLILKKDSRVETFNWLL
                     ENLDLYPTHFRKLKEESKVLFDTLESMKDKLFRTIRNYLEEMRRVYDVLLSDVNFFDD
                     SKESVTCILSGEQLAKKMSSYYGKTYDIDKCNKLLSLMTFIGLIKKLDDEDVPLKMLE
                     YLNRLKEEKALGNFNHKRSNVYRLDVFNATSVIQKLNEDILPLLEKIHFTYQYFSYEW
                     VKICFNEKEAKRVFPQNSRSELSKEKQLILDEAHEVLQGLSWQGTYLMTEKELINTVC
                     ERLRNVGDNSVKNALIKNRGYFVKQGFTLERASKDIKKLYNVKASSSFLIYLAKGAGI
                     DETIKELILSGAGIVPLYQFTSVTRDDGRRVRKRKDPIVLN"
     gene            complement(6123..6458)
                     /locus_tag="IXT20_002496"
     CDS             complement(6123..6458)
                     /locus_tag="IXT20_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ4860913.1"
                     /translation="MKQKQYAKVIREKRKALGWTQKELAEKIFSTQQAVARWENSVTE
                     PNLDSLTALSRALGTPVSHFLDNVVVDYEEEFLALYRSLSTEDAVRTIDYMKLLKRQE
                     NERNQLLKD"
     gene            6585..6800
                     /locus_tag="IXT20_002497"
     CDS             6585..6800
                     /locus_tag="IXT20_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ4860914.1"
                     /translation="MKKVSNLSRYRKEKGLSQTELAKKMNVTQQCVSSWQTGRTIPKP
                     YQMKMLSEILSVPMNELFSDVFNKVNS"
     gene            6837..7199
                     /locus_tag="IXT20_002498"
     CDS             6837..7199
                     /locus_tag="IXT20_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010717431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HAQ4860915.1"
                     /translation="MYCRKCGKEITDESKYCQFCGALQETDETTGTDTVKKSTEQTIN
                     EMKEQIVHGQETGKLYLIIGWISMVVSLAFIPVVFGAVAVIMGSLYREKDEKMGTILM
                     IAGVAGAIFGVLLGMSMY"
     gene            7245..7571
                     /locus_tag="IXT20_002499"
     CDS             7245..7571
                     /locus_tag="IXT20_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4860916.1"
                     /translation="MNKVNMNKKFVIIGIVVLAIIGIIFSKMTGTSGLEKSSKPLVTQ
                     ILQEQYNLNRSCDDVTITHENGDNTYRAKAILDNGSAININIEYYPKKDRIYVEIPYA
                     EVLMLN"
     gene            complement(8170..>8379)
                     /locus_tag="IXT20_002500"
     CDS             complement(8170..>8379)
                     /locus_tag="IXT20_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HAQ4860917.1"
                     /translation="KLDHSLLGKQRKTVETVFSSLEKLGCQNFNSRSVKGLESRFESI
                     LLAYSVLLSRAQRRFEGTLRYSLGY"
BASE COUNT         2820 a         1213 c         1555 g         2791 t
ORIGIN      
        1 actaaaaaaa tgatggagac aatcggtccg gcattatccg ctcaacgagc agtcatgcaa
       61 aacattgata ctgaggagtt cagacgagca actgagagaa tgaagcagtc tctatcacca
      121 aagcaagcat tgaaatccga ttataaaatt gctgatgata ttgaagatag taataaataa
      181 agagtccaga actttttcca attaataaac tattaaaaga attagagagg tgaaataaaa
      241 ctagaaaaat acaatctatt tggtaaaaat catggaggta aataatgggt gaacgaactt
      301 ttgaagatgt taaagactat gtagaattgc agagccaaga aaagtgtacg gtgttaagtg
      361 ccaagccgga acaggaattt aatgatcttg gtacagaagt aacggtttgg aatgtcaaaa
      421 cggatactga cggcgattgg tgggttgttg agggtgataa cgttcctatg aacctttatc
      481 cgcaaagtgc atactatttt ggaacagacg aggtatattc gtttcatatg ggtttaatgg
      541 agagaatgag agttttacat gaaaaatatg atccagagga ctttgtaagg agcgttactc
      601 ttgatacaga gattgcacca caactctttc ggaagctaaa gaatgttgct actcttatag
      661 acagtgcaga agaaattgaa gattttcaat ctattggtgt tcagtgtaga gaaattctaa
      721 tcgaacttgg taatcacatc tttatagcag atatggcagg tactaaagaa caaccacaaa
      781 aatcaaactt caaaaaaaag gcggaacttt ttatacagtt ttatttgaca ggttccgata
      841 actctgacta tcgaagtatt ataaaaaaac taactgaatc aacatgggac tacgctaata
      901 aattaactca ttctagtaca actactaagt atgaagcctc aacttgcgta atgcttgtaa
      961 cgtctttggt tggtgtgtat gaaaacataa cgcaaaaagt ctttgatcct gtctcacagt
     1021 atatatgttc gtcgtgtaaa agtaaaaaac tcacgataga ttctgatgat tctgatgaaa
     1081 acggaatggt tgaatccttg tatttaaaat gtgaagaatg tggtgatatt acaaaagtag
     1141 tatttttgac tagtgataag gatagttcat acacaaaagg aaaaataatt gagtaatgat
     1201 tatggatcat gtcaaaaaca tatcacgcaa ttcaaagtgc acacgttttt aaatttgata
     1261 cctcaaataa cttctctacc tgcttttttg ggtacgatat tggatacgtt ctgggggata
     1321 tcaagtgctg tgataggctg tcagtgatat ataccagagc gggaaaaatg cttttataac
     1381 gcgaatcatg taccaatgct attgaaaaga gtcattggaa ggcagtgttg ggtaaacctg
     1441 ttataggagc tagaaccctt gatatataag ggttctagct tttttgtttt gcctgtatac
     1501 tagaaatgac cagattttga ccagctcttt tactgatgag ccatcatatc agcaaagtct
     1561 tgacctgttt tcttaggtgc attatttgta atatgtgcat agatgtctaa tgtcacttta
     1621 ctattttcat gccctaatcg ttgttgaact accttgacag attctcctgc ttctaataat
     1681 aaactagcat gtgtatgtct aaacccatga ggtgtaatac gaggcaagtt atattttttc
     1741 aaaatatatc ttaaccaatc attaacagtg tttggaatgt atagagtgtt tttaactgtt
     1801 gtaaaaacat gttgctcttt agagctagta ttgtatccaa gttttaaata ctcttctttt
     1861 tgctgtaatt tccagttgtt gaggatagaa agagtctcag tatctaaaga gataactcgt
     1921 ctagaagctc ttgttttcgg agattgaatt ataactttac caaattcatc tacagcaaga
     1981 gttttattga catgtagttc tttattaaac gtgtctatgt ctttccactg taaagcgagt
     2041 acttcactct ttctcatacc tgtgtatgcc aataatctga agaaggcgta ttgtttcatg
     2101 ttaccaaaat ctttaaaagc atccagtaaa ttattaagct cctcttttga gtaaaattta
     2161 cgatttttct catactcttt ttttcgtgga agaagtgttt tttccatagg attattgtca
     2221 atatattgca ttgcaacccc gtagcgtaaa atttgtgcag tctgttttct catataatcg
     2281 taactttcat atttactgtg ccattcatct accacttttt gacaatatgg gacagagata
     2341 ttcgatagtt taagcttacc aaatgctgga agtatttgat tatttgcgta tcttactgag
     2401 attgcgattg ttgaagcttt aacactggta cgctgatgag ttatccattc attatacagg
     2461 tcttcaaatg tcatatcagg tgctttcttt ttaacaccgt ataatatttg atgtctgtac
     2521 tcgtcataat ctttttgagc ttcttgtttg ctactaaaac ctcttctcgt agtatttatt
     2581 ttttcaccag ttcgtggatc ggtggcaatg tgtcctttat acatccacgc cacttgaccg
     2641 tttttctttt tgtactttga tatttttgcc attaaatcat ctcctcttag tttacaagga
     2701 gtacttacca ctagatggta tcacctccct tcattttttt cttagtgtat tttttcccaa
     2761 ttgatgaaat cgtttgttct agcttgttta tattatcagt aatttctctc acagcttctt
     2821 cgggataaga catgtctttc aattgaatag gtagccattc atctaccatt ctttctagta
     2881 gttcttctat actgctagtg aggaaaagaa tgttattgtc aaattcttct acaatagcat
     2941 tgatgaataa ttcttctatt tttaagatat tgaaaagact aattttttca tttttaactt
     3001 tattacaagt cttgttagca ataaattctt gcatctctgg ttctaatttg gaatatctat
     3061 ccagccatgt atctaaatta gagtgtactg gtgggaactg ttgacgaaga tgctggaaca
     3121 ggtttttatc tggaaatgtt ataccatcta gtacaatgct atctctataa agaataattc
     3181 ccaaaatata ttcttctaca ttgccacaaa ctaactcctc tatactcata tttcccaaat
     3241 cagcaattcg ggataatcgc tctggtattg gtaaataggt tcccttctcc cattcgtaga
     3301 tagttttttt gtcaattaat tgaccgttgt taatcataag accaaatgct ggttgtgtta
     3361 attttttact ctttcttata gcttttattt ttgttccgag tatctttttt cgttctaaat
     3421 aatttattgg tggacgtttt atcatattga ccacacctcc taaaattagt atacctcaaa
     3481 aaaggggtgt tgtaaataaa ttttacaaag ctgttgacac ccctttttta tcgaggtatt
     3541 atgtgtatgt cgatgaactg gtaatcattt tttgtttgat taatcggtta ataacaaaat
     3601 acttttattt tgagcaaaaa atacgaagat aattcttaga aaatttgttc aagcaatcac
     3661 gaaaggaggg ggaaatatgt ctctacaaat cattgatagc attgtttcta tacaagttga
     3721 acaacaagtg aaagagataa ttacagtgct aaagcctatg ctagaagaca ttatttctgg
     3781 agtcactcta gagtatatgg actatcagca ggcaagtgaa tatctaggtg tatcagtagg
     3841 aacgattaga aaatatgttt ctcaatacgg gttacctgtt ataaaaattg atacggtcat
     3901 tagatttaaa aaatctgata ttgatagttt catggaacgg tataaaggtt gagtaaacaa
     3961 ataagactaa aacatatagt aataaagagt ttgtttttat tatctgtatg ttttagtctt
     4021 taacagaatt ttataaggag gaaacacaaa tggaaacatc aatgattaat attccaattt
     4081 atgtagatca agaaaaatta ttagagaagc cagttacaaa aaaagaaaaa actgatgtaa
     4141 gagataggtg tttagataac tatagagaag ttacaagaga tgagtttgta gagatactaa
     4201 attcatcaca atcatttatt ccaagcaaga caaagaataa aggcaatgac aaggagagtt
     4261 ttgtggaaac cagagtaatt attctggatg tcgataacac tgttaaagat gagaagggga
     4321 aggtagtaga tttgagtaaa gatgatagta gatacttaag catagaaaag gttttatcta
     4381 tcaatttagt tcataattct gcgtttgcga tacaaaaaag tattcggtac tctgaaaaca
     4441 tagaaaaata caagattgtc tttctattaa aagaagcaat tactgactat ggtaaaatgg
     4501 ttgctgttta cgaatacttg aaaaagaaca tccccgggtg tgatgacaat gtaaatgctt
     4561 cgaatagaat gttttttgga ggatataagt cagatgcagt gataataatt aatgaagaca
     4621 acatgttaga tattactgag cttccgattg attttaccat gacatgttct aaaaattgtc
     4681 aaagtgattc agggagagag tatgaactag tcaatgaaac caattttgtt aagttgataa
     4741 aaaatgacga caaagaagaa atgaaacagt ggtttaaaga ctgcctgttt gattcctccg
     4801 acatgtcttt taatgagatt tatgaaagat tgttaaccat tgatatgaat aaattactta
     4861 agtcaagtaa aaacttaaga tgtctgtttc acaacgatca taatccctct gctagtattt
     4921 ttaccagtaa aactgggcat tccatttttt attgtcattc cagtaattgt gatatcagtg
     4981 aaaattttat tggagttgtg atgctaattt taaagaaaga cagtcgggta gaaacattta
     5041 attggctatt agagaaccta gacttgtatc ctacacattt tcgaaaattg aaagaggaat
     5101 caaaagtact ttttgacact cttgaatcaa tgaaagacaa acttttcagg acaataagga
     5161 attatctaga ggaaatgcga cgtgtctatg atgtcttgct ttctgatgtt aatttttttg
     5221 atgatagtaa ggaaagtgtg acttgtattt tgtcggggga acaattagct aaaaaaatga
     5281 gttcttacta tggtaaaact tatgacatag ataaatgtaa taagttacta tcacttatga
     5341 cttttatagg attgataaaa aaattagatg atgaagatgt tcctttgaag atgctcgaat
     5401 acctaaatag gttaaaggaa gagaaagcgt taggaaactt taatcataag agaagcaatg
     5461 tctatcgatt agacgttttt aatgcaactt ctgtgattca aaaattgaat gaagatatcc
     5521 ttccacttct ggaaaaaatt cattttacat atcaatattt ttcttatgaa tgggttaaga
     5581 tatgttttaa cgaaaaagag gctaaaagag tatttcctca aaattcccga tcagaactca
     5641 gcaaggaaaa acaattgatt ttagatgaag cacatgaagt tttacagggc ttatcgtggc
     5701 aagggacata tctaatgaca gagaaagaac ttattaacac tgtttgtgaa cgcctacgta
     5761 atgttgggga taacagcgtg aaaaatgctt taataaaaaa cagaggctac tttgttaaac
     5821 aaggattcac tcttgaacga gcttctaagg atattaagaa actatacaat gtgaaagcaa
     5881 gtagtagttt tttaatatat ttagcgaaag gggcagggat agatgaaaca ataaaagaat
     5941 tgatattatc aggagctggg atagttcctc tgtatcaatt tacaagtgta acaagagatg
     6001 atggacggag agtaaggaag agaaaagacc caattgtatt aaactagaca ggttatccat
     6061 agctcttaat cttatttcat ctcaatacac ccaccgttaa agttcggtgg gttatttatt
     6121 tttcaatctt tcaatagctg attgcgttca ttttcttgcc tttttaatag cttcatatag
     6181 tcaattgttc gaactgcatc ttcagtgcta agagaacgat agagagccaa aaactcttcc
     6241 tcataatcta cgacaacatt gtctagaaaa tggcttacag gggttcctag ggctctagaa
     6301 agagctgtta agctatcgag gttaggctct gttacagaat tttcccatct tgctactgcc
     6361 tgttgtgtag agaagatttt ttcagctaat tctttttgtg tccacccgag agcttttcgc
     6421 ttttctctaa tgacctttgc atattgtttc tgtttcatta atacaccccc atctcagtat
     6481 agtacaacaa gttgttgtat ttgtaatggt atttgaagtt ttttatattt tatgagttga
     6541 aatacatcat attgttgtat ataataaaac aggaggggag aatcatgaaa aaagtaagta
     6601 atctcagccg atacagaaaa gaaaaaggac tttctcaaac ggagttagca aaaaaaatga
     6661 atgttacaca gcaatgtgta agttcgtggc aaacagggag aacgattcct aaaccctatc
     6721 aaatgaagat gctttctgag atattaagtg taccaatgaa tgagctgttt tctgatgtat
     6781 ttaataaagt aaacagttaa ctaataagca attgaatgat tttaggagga gaagacatgt
     6841 attgtagaaa atgtggcaaa gaaataacag atgaaagtaa gtattgccag ttttgtggag
     6901 cactacaaga aacagatgag acaaccggaa cagacacagt aaagaaaagt actgagcaga
     6961 caattaatga aatgaaggaa caaatcgtac atggacaaga gacagggaag ctgtatttga
     7021 ttattggttg gatttctatg gtagtatcgt tagcatttat tcctgttgta tttggtgctg
     7081 ttgctgttat tatgggctcc ttatatcgag aaaaagatga gaaaatgggt acgattttga
     7141 tgatagcagg ggtagctgga gcaatctttg gtgtactttt agggatgtca atgtactgat
     7201 aagagggata agagcaaatt attttggaga taggagataa aaagttgaat aaagtaaata
     7261 tgaacaagaa gtttgtcatt ataggtattg ttgtcttggc tattataggt attatttttt
     7321 caaaaatgac aggtacatct ggattagaga agtcatcaaa accattggta acacagattt
     7381 tacaagaaca gtacaactta aatcgatcat gtgatgatgt gacgattact catgaaaatg
     7441 gagataacac ataccgggct aaagctatat tagacaatgg aagtgctatc aacataaata
     7501 ttgagtacta tccaaagaaa gaccgtatat atgtggagat accatatgct gaggtgttaa
     7561 tgttaaattg atttaaatgt attatgcata ccgtccaaac aggaactccc aaaggtgtaa
     7621 attcactagg gagtttttat agtttcctat aaattaaata ctaatacagg tgacaaaaaa
     7681 atattacaat gattgtcggg aatttttatg ttattcactg ttataacaat ataaaagaaa
     7741 tgaaaaagaa aaaattatta tggatatgtt tggtattttt attgataatt ggtggaattg
     7801 aaatacttac tacatgtaga ttagaaataa atcagtttaa acatttgcta gttaattttt
     7861 aagaatacta gcccaaatca ttctatcgat acctttatat ctcggaatga ttgaaattgg
     7921 ctattgctta ctgattttta ccaaaaaaac aagtatcact attgttgcct atattgttgg
     7981 aatgctgttt attccatcct ttactcacca aattcatcaa tttttcccaa gagcagaatt
     8041 gttgaagttg tgtgatccgt tatatgcatt tttgatgctt tcacgatttt gggaatttcc
     8101 cgctggttat gtaggaatgg ttttactggt atagttaata ttagatataa cccgtggtgc
     8161 tagttaattt taatatccta aagaatatct caaagttcct tcaaaacgtc gttgtgctcg
     8221 acttaataga acactgtaag ccagtaatat gctttcaaat ctactctcta gccctttaac
     8281 agaacgtgag ttgaaatttt gacaacctaa tttttctaag gaagaaaaaa cagtctcaac
     8341 tgtttttctt tgttttccaa gtaagctgtg gtctagttt
//
LOCUS       DACGLE010000097         8251 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0534
            SAMEA3304449-rid9560453.denovo.102, whole genome shotgun sequence.
ACCESSION   DACGLE010000097 DACGLE010000000
VERSION     DACGLE010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304449
            Sequence Read Archive: ERR985064
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8251)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8251)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702225.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702225.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 20:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,045
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 27 of 67
            Pseudo Genes (incomplete)         :: 25 of 67
            Pseudo Genes (internal stop)      :: 19 of 67
            Pseudo Genes (multiple problems)  :: 4 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8251
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304449-rid9560453.denovo.102"
                     /strain="VREN0534"
                     /db_xref="taxon:1352"
     gene            <1..197
                     /locus_tag="IXT20_002501"
     CDS             <1..197
                     /locus_tag="IXT20_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4860918.1"
                     /translation="WFLSNAQSLVLLAIVVIGLYLGFKREFSKLIGFLIIAIIAVGLV
                     FNAAGVKDILLELFNRIIGA"
     gene            314..811
                     /locus_tag="IXT20_002502"
     CDS             314..811
                     /locus_tag="IXT20_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000342537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HAQ4860919.1"
                     /translation="MDDMQVYIANLGKYNEGELVGAWFTFPIDFEEVKEKIGLNDEYE
                     EYAIHDYELPFTVDEYTSISELNRLWEMVSELPEELQSELSALLTHFSSIEELSEHQE
                     DIIIHSDCDDMSDVARYYIEETGALGEVPASLQNYIDYESYGRDLDLSGTFISTNHGI
                     FEIVY"
     gene            898..1290
                     /locus_tag="IXT20_002503"
     CDS             898..1290
                     /locus_tag="IXT20_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAQ4860920.1"
                     /translation="MKKIRSYTSIWSVEKVLYSINDFRLPFPITFTQMTWFVVSLFAV
                     MILGNLPPLSMIEGAFLKYFGIPVAFTWFMSTKTFDGKKPYGFLKSVIAYALRRKLTY
                     AGKKVTLGRNQPQEAITAVRSEFYGISN"
     gene            1274..3721
                     /locus_tag="IXT20_002504"
     CDS             1274..3721
                     /locus_tag="IXT20_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAQ4860921.1"
                     /translation="MAYPIKYIENNLVWNKDGECYAYYELVPYNYSFLSPEQKIQVHD
                     SFRQLIAQNRDGKIHALQISTESSILSAQERSKNEVTGKLKTVAYDKIDQQTDALISM
                     IGENQVDYRFFIGFKLLLNDQEFSMKSLTVEAKNAFTDFVYDVNHKLMGDFVSMSNDE
                     ILRFQKMEKLLENKISRRFKIRRLDKDDFGYLIEHLYGQTGTAYEEYEYPLSKKKLEH
                     ETLIKTYDLIKPTRCLVEEKQRYLKIQQEDETAYVAYFTINSIVGELDFPSSEIFYYQ
                     QQQFTFPIDTSMNVEIVANRKALSTVRNKKKELKDLDNHAWQSDNETSSNVAEALESV
                     NELETNLDQSKESMYKLSYVVRVSANDLDELKRRCNEVKDFYDDLSVKLVRPFGDMLG
                     LHEEFLPASKRYMNDYIQYVTSDFLAGLGFGATQMLGENEGIYVGYSLDTGRNVYLKP
                     ALASQGVKGSVTNALASAFVGSLGGGKSFANNLIVYYAVLYGAQAVIVDPKAERGRWK
                     ETLPEISHEINIVNLTSDEKNKGLLDPYVIMKNTKDSESLAIDILTFLTGISSRDGER
                     FPILRKAIRAVTNSEVRGLMKVIEELRVENTPLSTSIADHIESFTDYDFAHLLFSDGY
                     VEQSISLEKQLNIIQVADLVLPDKETSFEEYTTMELLSVAMLIVISTFALDFIHTDRS
                     IFKIVDLDEAWSFLQVAQGKTLSMKLVRAGRAMNAGVYFVTQNTDDLLDEKLKNNLGL
                     KFAFRSTDLNEIKKTLAFFGVDPEDENNQKRLRDLENGQCLISDLYGRVGVIQFHPVF
                     EELLHAFDTRPPVRKEV"
     gene            3724..5907
                     /locus_tag="IXT20_002505"
     CDS             3724..5907
                     /locus_tag="IXT20_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HAQ4860922.1"
                     /translation="MKPSILNRIKSNWTLKRLGKVAMTVAFTLVIAIFLLAMLGTVVQ
                     AAGLVDDTVNVANEYSRYPLENYQLDFYVDNSWGWLPWNWSDGIGKQVMYGLYAITNF
                     IWTISLYVSNATGYLVQEAYSLDFISATADSIGKNMQTLAGVTPSGLSTEGFYVGFLL
                     LLILVLGVYVAYTGLIKRETTKAIHALMNFVLVFILSASFIAYAPDYIKKINDFSSDI
                     SNASLSLGTKIVMPHSDSQGKDSVDLIRDSLFSIQVQQPWLLLQYNSSDIESIGIDRV
                     ESLLSTSPDSNNGEDREKIVAEEIEDRSNTNMTITKTINRLGTVFFLFVFNIGISIFV
                     FLLTGIMIFSQVLFIIYAMFLPVSFILSMIPSFDGMSKRAITKLFNTILTRAGITLII
                     TTAFSISTMLYTLSAGYPFFLIAFLQIVTFAGIYFKLGDLMSMFSLQSNDSQSVGSRM
                     MRKPRMLMHAHMHRLQRKLGRSVTALGAGSAIASATGKKGQSGSGSSASTQADHSRPN
                     GQEKSTLGKRIGQTIGTVADTKDRMVDTAGNLKEQVKDLPTNARYAVYQGKSKVTDNV
                     HDLTRSISQTKADKASGRKKQQEQRRKTIAERRSEMEQVKQKKQPASSVHERPATKQE
                     QSHDGQTSRQTTVQASYRESQQAKQERPTVKSDSSSLKTERQSNTVQERTVQKPVTST
                     APTDRASQRSITKERQSTVQRVPLQNTKSRPPIKTATIKKGSKKP"
     gene            5904..6905
                     /locus_tag="IXT20_002506"
     CDS             5904..6905
                     /locus_tag="IXT20_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018368130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase P60"
                     /protein_id="HAQ4860923.1"
                     /translation="MKLKTLVIGGSGLFLMVFSLLLFVAILFSDEQDGGFSNIHYGGV
                     DVSAEVLAHKPMVEKVAKEYGIEEYVNILLAIIQVESGGTAEDVMQSSESLGLPPNSL
                     STEESIKQGVKYFSELLASSERLGVDLESVIQSYNYGGGFLGYVANRGNKYTFELAQS
                     FSKEYSGDEKVSYPNPIAIPINGGWRYNYGNMFYVQLVTQYLVTTEFDDDTVQAIMDE
                     ALKFEGWKYVYGGASPTTSFDCSGLTQWTYGKAGINLPRTAQQQYDVTRHIPLLEAKA
                     GDLVFFHSTYNAGSYITHIGIYLGDNRMFHAGDPIGYADLTSSYWQQHLVGAGRIKQ"
     gene            6920..7834
                     /locus_tag="IXT20_002507"
     CDS             6920..7834
                     /locus_tag="IXT20_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000975335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAQ4860924.1"
                     /translation="MMKFGKNQNTEKQTPKEKKPRVYKVNPRKKVVIVLWVLLGLSFS
                     FAIFKHFTAIDTHTIHETTIIEKEYVDTHHVENFVENFAKVYYSWELNDKSIDNRIEN
                     LKAYLTEELQALNVDTVRKDIPVSSSVKGFQIWTVEADGDNQFDVTYSVDQLITEGEN
                     TKTVHSAYIVSVYVDSTGNMVLIKNPTITSIPKKSDYKPKAIESEGTVDSITTNEINE
                     FLTTFFKLYPTATASELSYYVNDGILKPIGKEYIFQELVNPIYNRKDNQVTVSLTVEY
                     IDQQTKATQVSQFDLVLEKNGSNWKIIE"
     gene            8079..8195
                     /locus_tag="IXT20_002508"
     CDS             8079..8195
                     /locus_tag="IXT20_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance determinant leader
                     peptide"
                     /protein_id="HAQ4860925.1"
                     /translation="MILKYPENICMLCMPMVMHKNPSDKSIYHWDFYALLGF"
BASE COUNT         2569 a         1568 c         1754 g         2360 t
ORIGIN      
        1 actggtttct atcaaacgct caatcactgg tgcttttagc aatcgttgtg attggcttgt
       61 atcttggctt caagcgtgag tttagcaaac tgattggctt tttaattatt gcgattattg
      121 cggttggctt agtcttcaac gctgctggag taaaagacat tttactagag ctattcaatc
      181 gcattattgg tgcttaaata aaaccgttct tttgtggaat ataagtggtt ttcttatgtt
      241 ccgcaaagga atggtacacc aaacgaagtg cggtagggat ttttgaatct ctacaaagaa
      301 aggacgtgaa tatatggacg atatgcaagt ctatattgcg aatttaggca aatacaatga
      361 gggcgaattg gtcggtgcgt ggtttacctt tcccattgac tttgaggaag tcaaagagaa
      421 aatcggcttg aatgatgaat atgaggaata cgccattcat gattacgagt taccctttac
      481 ggttgacgaa tacacttcta ttagcgaact caatcgccta tgggaaatgg tatcggagtt
      541 gccagaagaa ctacaatcgg agctatctgc tctgctcact catttttcaa gtattgaaga
      601 actaagcgaa catcaagaag atattatcat tcattccgat tgtgatgata tgtctgacgt
      661 ggcacgctac tacattgaag aaacgggtgc tttaggcgaa gtgccagcca gtcttcaaaa
      721 ctatattgat tatgaatcct atggtcggga tttagacctt tcgggaacgt ttatctcaac
      781 caatcacggg atttttgaaa tcgtctatta aatctgtcgg tacattctta ctggcagatt
      841 ttcttgttta cggggtagtc aaaagactat ccccattttt atgaaaggat tgattacatg
      901 aagaaaatac gaagctatac cagtatctgg tctgttgaga aagtgttgta ctccatcaat
      961 gactttagac ttccgtttcc cataaccttt acgcaaatga catggtttgt cgtgtcactc
     1021 tttgcagtta tgatacttgg caacttgccc cctctttcta tgatagaggg agcatttctc
     1081 aaatactttg ggattcctgt ggctttcaca tggtttatgt ctacaaaaac ctttgatggt
     1141 aaaaagcctt atggattttt gaagtctgtc attgcttatg cactgcgacg aaagctgacc
     1201 tatgcaggaa aaaaagtaac gcttggcaga aaccagccac aagaagccat tacagcagtt
     1261 aggagtgaat tttatggcat atccaattaa atatattgaa aacaatctgg tctggaataa
     1321 agatggggaa tgttacgctt actatgagct tgttccctac aattactcat ttctaagtcc
     1381 ggaacagaaa atacaagtac atgattcttt cagacagctt atcgcacaaa atcgtgatgg
     1441 caagattcat gctttacaaa tcagtacaga atccagcata ctttctgcac aagaacgttc
     1501 caaaaatgaa gtcactggaa agctcaaaac ggttgcctat gacaaaatcg accaacagac
     1561 agacgcttta atatccatga ttggcgaaaa tcaagtggac taccgtttct ttatcggttt
     1621 taagttgctt ctcaacgatc aggagttttc tatgaaaagt cttaccgttg aagcaaaaaa
     1681 tgcttttact gattttgtct atgatgtgaa ccataagctg atgggcgatt ttgttagtat
     1741 gagtaatgat gaaatcctgc gttttcagaa gatggaaaag cttttagaaa ataaaatctc
     1801 ccgtcgtttc aaaatccgaa gattagataa ggatgacttc ggctatctga ttgaacacct
     1861 ttacggacag acaggcactg cctatgaaga gtatgagtat cctctatcaa agaaaaagct
     1921 ggaacatgaa acactgatta aaacctatga cctcattaag cctactcgct gtctggtgga
     1981 agaaaaacag cgatatttga aaatccagca ggaagacgaa accgcctatg tagcttactt
     2041 taccatcaac agtattgtcg gcgaactgga cttcccgtcc tctgaaatct tctactacca
     2101 gcaacagcaa tttacctttc cgattgatac gtcaatgaat gtggaaattg tagcgaatcg
     2161 taaagcccta tctactgtcc gcaataaaaa gaaagaactg aaagacttgg ataaccacgc
     2221 ttggcaaagt gataatgaaa ccagctctaa tgtggcggaa gctctggaaa gtgtgaacga
     2281 gctggaaacc aatttagacc aaagcaagga atctatgtat aagctgtcct atgttgtaag
     2341 ggtatcagca aatgaccttg acgaacttaa acgtcgttgt aatgaagtga aagatttcta
     2401 tgatgatttg agcgtgaaac tggtacgacc ttttggggat atgctgggct tacatgaaga
     2461 atttttacct gccagcaaaa gatatatgaa tgactatatt caatacgtga cctctgattt
     2521 cctcgctggt ttaggttttg gtgctactca aatgctgggt gaaaatgagg ggatttatgt
     2581 tggctacagc ttagatactg gacgcaatgt ctatctgaaa cctgctcttg ccagtcaagg
     2641 ggttaagggt tcagtaacca atgcgttagc gtctgccttt gtcggttcgc tgggtggtgg
     2701 taaatccttt gcgaataacc ttatcgtcta ttatgcagtg ctttatgggg cacaagcagt
     2761 gattgtagac ccaaaagcag aacgtggcag atggaaagaa accttgccag agatttccca
     2821 tgaaatcaat atcgtcaatc tgacttctga tgagaaaaac aaaggcttac ttgaccctta
     2881 tgtaattatg aaaaatacca aagattctga atcactggct attgatattt tgacattcct
     2941 tacggggatt tcctctcgtg atggggaacg cttcccaatc cttagaaaag ccattcgtgc
     3001 agtaaccaat agtgaagtgc gagggttgat gaaagtgatt gaagaattac gggttgagaa
     3061 tacgccacta agtaccagca tagccgacca tatcgaaagt tttacagact atgactttgc
     3121 ccatctgctt tttagtgatg gttatgtaga gcagtctatc agccttgaaa aacaactgaa
     3181 cattatacag gttgccgact tggtacttcc tgacaaggaa acttcctttg aggaatatac
     3241 cacaatggag ttactatcgg tagcaatgct gattgtcatt agtacctttg cgttggactt
     3301 tatccataca gaccgaagta ttttcaagat tgtagactta gacgaagcat ggagcttctt
     3361 acaggtggca caagggaaaa cactatctat gaagctggtt cgtgctggtc gtgctatgaa
     3421 cgctggggta tatttcgtga cccaaaatac agatgacctc ttagatgaaa aactgaaaaa
     3481 taacctcggc ttaaaatttg cctttcgttc cactgacctt aacgagatta aaaaaaccct
     3541 tgcctttttt ggtgtagacc cagaggacga aaacaatcag aagcgattgc gtgatttgga
     3601 aaacgggcaa tgccttatca gtgatttata tggtcgtgtc ggtgtgatac agttccaccc
     3661 tgtatttgaa gaactgctcc atgcctttga taccagacca cctgtgcgaa aagaggtgta
     3721 aatgtgaaac catcaatatt aaacagaata aaatcaaact ggacgctgaa acgtctaggt
     3781 aaagtggcaa tgacagtggc tttcacactt gtgattgcca tttttctttt agccatgctg
     3841 ggaacggtgg ttcaagctgc gggcttggta gatgatacgg tcaatgtggc aaatgaatac
     3901 agccgatacc cacttgaaaa ctatcaactg gatttttatg tggataatag ctggggctgg
     3961 ctaccgtgga actggtcgga cggaattgga aaacaagtca tgtatggact atatgccatt
     4021 accaatttta tttggacaat cagtctttat gtttccaatg cgacgggtta cttagtacag
     4081 gaagcctatt ccttagactt catttccgct acagcagatt ccattggcaa gaatatgcag
     4141 acccttgcag gggttacgcc tagtggacta tcaacagagg gtttctatgt tggattcctt
     4201 ttactcttga ttttggttct tggggtttat gttgcctata caggactgat aaagagagaa
     4261 accacaaagg caattcatgc cctcatgaat tttgtgctgg tttttatcct atcggcttcc
     4321 tttattgcct acgctcccga ctacattaaa aaaatcaatg acttttcatc agacatcagc
     4381 aacgctagtt tgtcactggg tacaaaaatt gtcatgcccc attcagatag tcaaggcaag
     4441 gacagtgtgg acttgatacg tgacagccta ttttccatac aggttcagca accgtggcta
     4501 ctgcttcaat ataacagttc agacattgaa agtattggta ttgaccgtgt ggaaagcctg
     4561 ctctcaacca gtccagattc caacaatggg gaagacagag aaaaaattgt tgcggaagaa
     4621 attgaagata gaagcaatac caatatgacg attacaaaga cgattaaccg tttaggtaca
     4681 gtcttcttcc tatttgtctt caatattggg atttccatat ttgtattcct attaacagga
     4741 atcatgattt tctcgcaggt actttttatc atctatgcta tgtttctgcc tgtgagcttt
     4801 attttaagca tgattccatc atttgatggt atgtcaaaac gagccataac aaagctcttt
     4861 aataccattt tgacacgagc tggaatcaca ttgattatta cgacagcatt tagtatttca
     4921 accatgctct ataccttatc ggctggttat ccgttctttt tgattgcttt tctacagatt
     4981 gtgacctttg caggaatcta ctttaagctg ggcgatttaa tgagtatgtt ttctctacag
     5041 agtaacgatt ctcaaagtgt gggaagtcgt atgatgagaa aacctcgtat gcttatgcac
     5101 gctcacatgc accgtctaca gcggaaactt ggacgctccg tgactgcttt aggggctggg
     5161 tctgccattg ccagtgctac gggtaaaaaa ggacagtcgg gttcgggaag ttctgcgagt
     5221 acacaagcag atcactctcg accaaacggg caggaaaaat caacactagg aaagcgtatc
     5281 ggtcaaacca tcggtacagt agctgatacc aaagacagaa tggtagatac tgctggtaat
     5341 ttgaaagaac aggttaagga tttgccgacc aatgcaagat atgcagtata tcaaggaaaa
     5401 tctaaagtga cggataatgt ccatgattta accagaagta tttctcaaac caaagcggat
     5461 aaagccagtg gacgcaagaa acagcaggaa caaagacgga aaaccattgc ggagcgtcgc
     5521 tctgaaatgg aacaggtcaa acagaaaaaa cagccagctt cttccgttca tgaaagacca
     5581 gccactaagc aagaacaatc tcatgatgga cagacttcaa ggcaaacaac tgtacaggct
     5641 tcctataggg aatctcaaca agccaaacaa gagcgtccaa cagttaagtc cgattcttca
     5701 agtttaaaaa cggaacgtca aagtaataca gttcaagaaa gaacggttca aaagccagta
     5761 acttcaactg caccaacaga tagagcttca caacgttcaa tcacaaaaga acgtcagtct
     5821 actgttcaaa gagtaccact acaaaataca aaaagtagac caccaatcaa aaccgccacc
     5881 attaagaaag gcagtaagaa accatgaagc tgaaaacttt agtgattggt ggttcgggat
     5941 tattcttgat ggtcttctca ctgcttctgt ttgtcgccat tttattttca gatgaacaag
     6001 atggcggttt ttccaacatt cactatggcg gtgtggatgt ttccgcagaa gtgctggctc
     6061 ataagcctat ggtagaaaaa gttgccaaag aatatggaat tgaagaatac gtcaatatac
     6121 ttcttgcgat tatacaggtg gaatcgggtg gtacagcaga agatgttatg cagtcctcgg
     6181 aatccctcgg tcttccacct aattcattga gtacagaaga atccattaag caaggtgtga
     6241 agtatttcag tgagttatta gccagtagcg aaaggcttgg tgtagattta gaatcggtta
     6301 tccagtccta caattatggt ggtggtttct taggatatgt ggctaatcgt gggaataaat
     6361 atacctttga actggctcaa agtttctcaa aagagtattc gggtgacgaa aaagtgtctt
     6421 accccaatcc catagccata cctatcaatg ggggctggcg atacaattac gggaatatgt
     6481 tttatgtgca actggtaacg cagtatcttg tcacaacaga gtttgatgat gatacagtac
     6541 aagccatcat ggacgaagct ctgaaatttg aaggctggaa gtatgtctat ggcggagctt
     6601 ccccgactac ctcttttgat tgtagcggac tgacacaatg gacgtatgga aaagctggaa
     6661 tcaacttacc acgaacagca caacagcaat atgatgtaac ccggcatatc ccattattgg
     6721 aagcaaaagc cggcgatttg gttttctttc attctaccta taacgctggc tcttatatta
     6781 ctcatatcgg gatttattta ggcgataacc gtatgtttca tgcaggcgac cctatcggtt
     6841 atgccgactt aacaagctct tactggcaac agcatttagt gggagcagga cgaatcaaac
     6901 aataagaaag gacgattcaa tgatgaaatt cggaaaaaat cagaatacag aaaaacagac
     6961 accaaaggaa aagaaacctc gtgtctataa ggtcaatcct cgtaaaaagg ttgtgattgt
     7021 cctatgggta cttttaggac ttagtttcag ctttgcgata ttcaagcact ttacagccat
     7081 tgatacccat accattcatg aaaccaccat catagaaaag gaatatgtcg atactcatca
     7141 tgtggaaaat tttgtagaga actttgcgaa agtctactat tcatgggaac taaacgacaa
     7201 gtccattgat aatcgcatag aaaacctgaa agcatatctg acagaggaac ttcaagctct
     7261 gaatgtcgat acagttcgta aagatattcc tgtatcgtct tctgtaaaag gatttcagat
     7321 atggacggta gaagcagatg gcgacaatca atttgatgta acctacagtg tagaccaact
     7381 cattacagag ggggaaaata caaagaccgt ccactctgct tatatagtga gtgtctatgt
     7441 agacagtact ggaaatatgg tcctcattaa gaatccgacc attactagca taccaaagaa
     7501 atcagactat aaaccaaaag ctattgaaag tgagggtacg gttgattcca ttacaaccaa
     7561 tgaaatcaat gagtttttaa cgacgttctt caagctctat cctacagcga cagccagtga
     7621 actttcctac tatgtgaatg acgggatatt aaaaccaatc ggaaaagagt acatctttca
     7681 agaactggta aatcctattt acaatcgtaa ggataatcaa gtcacggtat cgctgacagt
     7741 ggagtatatc gaccagcaga ccaaagcaac gcaggtatct caatttgatt tggtacttga
     7801 aaagaacggg agtaattgga agattataga ataacaaata ttggtacatt attacagcta
     7861 ttttgtaatc acgtactctc tttgataaaa aattggagat tcctttacaa atatgctctt
     7921 acgtgctatt atttaagtat ctatttaaaa ggagttaata aatatgcggc aaggtattct
     7981 taaataaact gtcaatttga tagtgggaac aaataattgg atgtcctttt ttaggagggc
     8041 ttagtttttt gcacccagtt taagaatacc tttatcatgt gattctaaag tatccggaga
     8101 atatctgtat gctttgtatg cctatggtta tgcataaaaa tcccagtgat aagagtattt
     8161 atcactggga tttttatgcc cttttgggct tttgaatgga ggaaaatcac atgaaaatta
     8221 ttaatattgg agttttagct catgttgatg c
//
LOCUS       DACGLE010000098         8067 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0534
            SAMEA3304449-rid9560453.denovo.103, whole genome shotgun sequence.
ACCESSION   DACGLE010000098 DACGLE010000000
VERSION     DACGLE010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304449
            Sequence Read Archive: ERR985064
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8067)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8067)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702225.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702225.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 20:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,045
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 27 of 67
            Pseudo Genes (incomplete)         :: 25 of 67
            Pseudo Genes (internal stop)      :: 19 of 67
            Pseudo Genes (multiple problems)  :: 4 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8067
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304449-rid9560453.denovo.103"
                     /strain="VREN0534"
                     /db_xref="taxon:1352"
     gene            <1..883
                     /locus_tag="IXT20_002509"
     CDS             <1..883
                     /locus_tag="IXT20_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="citrate transporter"
                     /protein_id="HAQ4860926.1"
                     /translation="ISVPVALFSFMGSIMAGIFANIVNFKQYQTIYAGFNPAAESYTY
                     NDYFQIGMIGMIVSLVVVLTVANISMNKKKRYAMAANVPAEGGDAPMISWLAVLLPVL
                     GVVLLDLQIILGFILAGIWALLFTGKLRGGYKEICRQFAKLFTDGAVDVAPMVGFLMT
                     LAMFNNSAAYASPYFSAIFGDWIPQSPLVLAIVFAILTPLGFFRGPMNLVGSGSAILA
                     VVLAVNPTMSPAFLFPLFAITTIAPQHLDITQSWVAWGLGYTKVTSREYMKKSIPTGW
                     IIGAILCLITFLLYGNA"
     gene            931..3060
                     /locus_tag="IXT20_002510"
     CDS             931..3060
                     /locus_tag="IXT20_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HAQ4860927.1"
                     /translation="MKRFVRMGIDVGGTHTKAVAIDNATHEIIGKSSVKTTHDDVRGV
                     AAGVVQSFQNCLRENNISPEDVVFVAHSTTQATNALIEGDVAKVGVIGMAKGGLEGFL
                     AKRQTRLNDIDLGNKKKIEIVNAFLPVKNLNVDRVSETISSLERERAEVLVSSMAFGV
                     DNGEPERVVYEAASVKAIPTTMASDITKLYGLTRRTRTAAINASILPKMLDTATSTED
                     SVREAGVNVSLMIMRGDGGVMEINEMKKRPVLTMLSGPAASVMGSLMYLRASNGVYFE
                     VGGTTTNIGVIKNGRPAIDYSIVGGHPTYISSLDVRVLGVAGGSMVRANQSGIIDVGP
                     RSAHIAGLDYAVFTETEKIKGPKVEFFSPKEGDPADYVKVVMEDGEEVTITNTCAANV
                     LGLVQEEHFSYGNVPSARKAIQALADYCHTTVEDIAEQIMEKSYAKIEPVILELADKY
                     HLEKDQISLVGVGGGAASLITYFSNKMGVKYSIPENAEVISSIGVALAMVRDVVERII
                     PSPSKEDIRSLKNEAMNKAIESGATPESIEVHVEIDPHTSKVTAIATGSTEVKATDLT
                     KEITTEEALELAAEDMRLNKNEVCLLENTPFFYVCGEQNRSKNAGSLRIIDQKGFIKV
                     QRGHASCMKTTAANYMTAVEQLWEDMAVYQTELIARPEFYLCLGARVSDFTATDLEQL
                     QLLMDLEVSTMEPEEEVIVVAGNIKQT"
     gene            3035..3724
                     /locus_tag="IXT20_002511"
     CDS             3035..3724
                     /locus_tag="IXT20_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4860928.1"
                     /translation="MPEISNKHKLEELLNELQQIPEEIWGFYQFRRDLFWKKIPLSKQ
                     KILIQQSIDCGIETACSIKKKYPFADVGEICEQMAVPIVSCESEQINERITFATYAED
                     EGIRLMTEPLEKLKCSGLASISKETAQALIIGHELFHHIEASVKGIYTQSEKIVLWRL
                     PFYTHQSNIRALSEIAAMSFSKEMNQSDFSPYVLEAVLLWPYNEIRSQGILEEIKEIE
                     KRCTEYDLDYK"
     gene            complement(4132..4818)
                     /locus_tag="IXT20_002512"
     CDS             complement(4132..4818)
                     /locus_tag="IXT20_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ4860929.1"
                     /translation="MVINNLQQQAYESIRKQIIYSELAPGSKISDKVLEEQLAIGRTP
                     IREALIQLRNQELVKTIPQSGTYVSKIDIRSASLARYTREKLEKPILQECSAKMTKQD
                     QAKLEKILEQTDQAVIAQNKKAFFDLDTDFHRTCYEIAGKREIWDWLESYSTHLNRFR
                     WLRLTISELDWGRVLDEHQTMLQSMIDHNFDEVGFLCTMHLHMIIEEQEYVIHNYPDY
                     FEDIQDRPIK"
     gene            complement(4954..5148)
                     /locus_tag="IXT20_002513"
     CDS             complement(4954..5148)
                     /locus_tag="IXT20_002513"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4860930.1"
                     /translation="MKRKKQVIPMYVVKVMHGYIDKTGCRTREKNLDNLLIFKDKKES
                     EAFAKRIGGRVKPIQEVRPD"
     gene            complement(5198..5638)
                     /locus_tag="IXT20_002514"
     CDS             complement(5198..5638)
                     /locus_tag="IXT20_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HAQ4860931.1"
                     /translation="MEKFTGYVREPFYYETDQMGIIHHSNYIRWFEEARVALLVHLGF
                     PYKKIEESGIIIPVLEVNCSYKEMIHYGEKVSIHPTIQKYTGTRLDFSYEIVGTEDKK
                     LKTTGSSKHCFLLSDSQRLVKLSKVNPELEQLFQAYASLDDVNS"
     gene            complement(5642..6487)
                     /locus_tag="IXT20_002515"
     CDS             complement(5642..6487)
                     /locus_tag="IXT20_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HAQ4860932.1"
                     /translation="MLINEQLVKRVITPRPKISHKGTFGRIVLIGGNEQYGGAIIMSA
                     LAAVHSGAGLTTVVTDEKNRSALHSHLPEAMIVDWTDTDQFLSVIKQADVLLIGPGLG
                     TSSKSLERFKQVLENQSKEQWLVIDGSALSLLVANHLLLPYPEQTVLTPHQMEWQRLS
                     SLPIQEQRDKENKKKQQEIGAVLVLKSHETKIYGHQHFYTNPLGSAAMATGGMGDTLA
                     GMITGFLAQFPQNDQTIAAAVYLHSFIGDQLAKEQYIVLPTQISEALPKWMNYFCKKS
                     MENEA"
     gene            6636..8054
                     /gene="pepV"
                     /locus_tag="IXT20_002516"
     CDS             6636..8054
                     /gene="pepV"
                     /locus_tag="IXT20_002516"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HAQ4860933.1"
                     /translation="MTIDWQKEVEARKEDLLEDLKNLLRVNSERDDSKVTPDAPFGPG
                     PRDALKHMLAYGERDGFKVKNVDNYAGHIDLGEGDETLGIFGHMDVVPAGDGWDTDPY
                     EPVIKDGKIFARGSSDDKGPSMAAYYAMKIIKELDLKLSKKVRFVVGSDEESGWGDMA
                     YYFEHEEEPDFGFSPDAEFPIINGEKGNVSLALRFKGDNAGDYVLKSFVSGLRENMVP
                     GTATAALQVPSADAAIAMEEAFYQFIEANPVSGTIEADNTYVKIELVGKGAHGASPQS
                     GINAGSFLALFLDNYEFLGSAKQFIHVAAAYVHEDFYGEKLGVAYEDEKMGKLTMNAG
                     LFAFEENGTEEANFINMNFRFPKGVTVDGLQSDIEQTVGQEGATVTRGARVMEPHYVP
                     MDDPLVATLLQVYEDHTGEKGYEQIIGGGTYGRLLKRGVAYGAMFPGYTDTMHQANEF
                     MSLDDLFRATAIYADAIYRLAK"
BASE COUNT         2481 a         1515 c         1713 g         2358 t
ORIGIN      
        1 tatttcagta ccggttgcac tgttctcatt tatgggctcg attatggctg gaatctttgc
       61 taatatcgtc aatttcaaac aatatcaaac gatttatgct ggtttcaatc cagcggctga
      121 aagctatact tataatgact atttccaaat tggtatgatt gggatgattg taagcttagt
      181 tgtggtacta acggtcgcta atatatcaat gaacaaaaag aaacgctatg caatggcagc
      241 aaacgtacca gctgaaggtg gcgacgcacc aatgatctca tggttagccg tattattacc
      301 agttttaggg gtcgttcttt tagatcttca aatcatctta ggttttattc tagcaggtat
      361 ctgggcattg ctgtttactg gaaaattgcg cggaggttat aaagaaatct gtcgtcagtt
      421 tgcgaaatta tttacagatg gcgcagtcga tgttgcacca atggttggtt tcttgatgac
      481 cttagcgatg ttcaataatt cagctgcgta tgcttctcct tatttttcag ctatttttgg
      541 agattggata ccgcaatcac cattagtctt ggcaatcgta tttgctatat tgacaccact
      601 aggatttttc cgcggtccaa tgaatctagt cggttcaggc tcagctattt tagctgtcgt
      661 gttagctgtt aatccaacga tgtcgccagc ctttttgttc ccgttatttg caattacaac
      721 gattgctcct cagcatttag atattacaca atcttgggtt gcttggggat taggttatac
      781 aaaagtaact tctcgagaat atatgaagaa atccatccca actggttgga tcatcggtgc
      841 gatcttatgc ctgatcacgt tcttattgta cgggaacgcc tagtaaacaa aacgtcgata
      901 aaaacgatta gataaatgga ggaaaagaaa atgaaacgtt ttgtgcgaat gggaattgat
      961 gttggcggta cacatacgaa agcagtcgca atcgataatg cgacgcacga aatcatcgga
     1021 aaatcttcag tgaaaacaac ccacgatgat gtccgtggag tagctgcagg tgtcgttcag
     1081 tcttttcaaa attgtttgcg ggaaaataat atcagtccag aagatgttgt gtttgttgca
     1141 cattctacca cacaagcgac taatgcactg attgaaggag acgtagcaaa agtcggcgta
     1201 atcgggatgg caaaaggtgg attagaaggt tttttagcaa aacgacaaac tagattgaac
     1261 gatatcgatt tagggaataa gaaaaaaata gagattgtca atgcattctt acctgtcaaa
     1321 aacttaaatg tagaccgtgt ttcagaaacg atttcttctt tagaaagaga aagagcggag
     1381 gtcttggttt cttctatggc atttggagta gataatgggg aaccagaacg agttgtttat
     1441 gaagcagcat ctgtgaaagc gatccctaca acgatggctt ctgacatcac aaaattatat
     1501 ggattgacaa gaagaactcg gacagcagca atcaacgcat ctattttacc aaaaatgcta
     1561 gatacggcaa cctcgacaga agactccgta cgagaagcag gggtcaatgt ttctttgatg
     1621 attatgcgcg gagacggcgg agtaatggaa atcaatgaaa tgaaaaaacg acctgtttta
     1681 accatgcttt ccggtcctgc tgcatcagtc atgggctctt tgatgtactt gagagcgtct
     1741 aatggtgttt attttgaagt aggcggaaca acaaccaata tcggggtcat taaaaatgga
     1801 cgtccagcaa tcgattattc aattgtaggt ggccacccaa catatatctc ttcattagat
     1861 gttcgggttc taggtgtggc aggcggttcg atggtacggg ctaatcagtc aggaatcatc
     1921 gatgtcggtc cacgttctgc acatattgcc ggtctagatt atgcagtatt tacggagaca
     1981 gaaaaaatca aaggtcctaa ggttgaattt ttctctccta aagaaggaga ccctgctgat
     2041 tacgtcaagg tagtgatgga agacggagaa gaagtgacta ttacaaatac atgtgcagcc
     2101 aatgttcttg gattagtgca agaagaacat ttttcttatg gaaatgtccc atctgcacga
     2161 aaagccatac aggcattagc agactattgt catacgacgg ttgaagacat cgcagagcag
     2221 atcatggaaa aatcctatgc taagattgaa ccggtgattt tagaattggc agacaagtat
     2281 cacttggaga aagatcagat ttctcttgta ggtgtagggg gcggcgctgc ctcattgatt
     2341 acttacttta gtaataaaat gggtgtgaaa tattcgattc ctgaaaacgc cgaagtgatt
     2401 tcttctattg gtgtagcact tgcaatggtc cgagatgttg tagaacgtat catcccttcg
     2461 ccaagtaaag aagatatacg atcgttgaaa aatgaagcga tgaataaagc gattgaatca
     2521 ggcgctacac cagaatcgat cgaagtccac gtagaaatcg atccacatac ttcaaaagta
     2581 acggctatcg ctactggttc tacggaagtg aaagcaaccg atctaaccaa ggagatcaca
     2641 acagaagaag cactagagct tgctgcagag gatatgcgat taaataaaaa tgaagtttgc
     2701 ttattggaaa atacaccttt cttttatgta tgcggcgaac agaatcgttc aaaaaatgct
     2761 ggaagtcttc ggatcatcga tcaaaaagga tttatcaagg tccaacgtgg acatgcttca
     2821 tgtatgaaaa caactgcagc caattatatg acagctgtcg aacaactttg ggaagatatg
     2881 gcagtttatc aaactgaatt gattgcccga ccagaatttt atctctgctt aggagcaaga
     2941 gtctctgatt ttacagcgac ggatctggaa caactacagc ttttgatgga tctggaagta
     3001 tctactatgg aaccggagga agaagtgatc gtcgttgccg gaaatatcaa acaaacataa
     3061 gttagaagag ttactaaacg aattacaaca gatccccgaa gaaatttggg gattctacca
     3121 gtttcggcga gatttatttt ggaaaaagat tcctttgtcc aagcagaaaa ttcttataca
     3181 acaatcaatc gattgtggta tcgaaacggc atgtagcatc aaaaaaaagt acccttttgc
     3241 cgatgtagga gaaatctgtg aacaaatggc tgttccaatc gtttcctgtg aatcagagca
     3301 aataaacgaa cgaatcactt ttgcaactta tgcagaagat gaagggatcc gattgatgac
     3361 ggagccgtta gaaaaattaa aatgttcagg actagcttcg atctctaaag aaacagcgca
     3421 agctttgatt attggacatg aactatttca ccacatcgaa gcttctgtta agggaattta
     3481 tacgcaaagc gaaaaaattg tgctgtggcg gttgcctttt tatacacatc aatcaaacat
     3541 tcgtgcattg agcgagatag cagcgatgag tttttcaaaa gagatgaatc aaagcgattt
     3601 ttcgccttat gtattagagg cagtcttact ttggccatat aatgaaattc gtagccaagg
     3661 gatcttagaa gaaataaaag aaatagaaaa aagatgtacg gagtatgatt tagattacaa
     3721 ataaagagac tgtgacaaaa gtcgtgtcac agtccttttt ttccgaataa acggtgttct
     3781 cctgtcaact cctcggtcac aagtcaaaat attcaacaaa catgagaagc tgttttttga
     3841 aattgttcct tgtgactcgg agatgaacgg caggctcacg acctcttgga aaaaggtgtt
     3901 caaaagcttg catctgcggt aataagaaca gccaagttaa aaacataaga agctattttt
     3961 acttggctgt tcttattact tgatgcaggg cagctttttc acaacctctc aataaacggt
     4021 gtgcaatcag ctgaccctcg gtattagctc aaaaaaaacg gcaaaatatg aggaactatt
     4081 ttgccgttta tttctctaat actcaggtca aaacgctgat tccaaaacct cttatttgat
     4141 gggtctgtcc tgaatatctt caaaataatc ggggtaatta tgaatcacgt attcttgctc
     4201 ctcgataatc atatgtaaat gcatcgtgca aagaaatccg acttcatcaa agttatgatc
     4261 aatcattgat tgaagcattg tctgatgttc atctaatact cgaccccagt ctaattctga
     4321 aatcgtcaaa cgtaaccatc tgaaacggtt caagtgcgta ctataacttt ctagccaatc
     4381 ccaaatttct cgttttccag caatttcata acaagttcgg tgaaaatcag tatccaaatc
     4441 aaaaaaagct tttttatttt gagcgatcac tgcctgatcg gtttgttcta gtattttttc
     4501 tagtttagct tgatcttgct tggtcatttt cgcactgcat tcttgcagga tcggtttttc
     4561 caacttttcc cgcgtataac gggctaatga agcagatcgg atatcgattt ttgaaacata
     4621 agtgccggac tgtggaatgg tttttacaag ctcttgattg cgcagttgga tcagtgcttc
     4681 tcgaatcggt gtacgcccga tagctagttg ttcttctaac actttatcgg agattttact
     4741 tccgggtgct aactcggaat aaatgatttg tttacgtata gattcataag cttgctgttg
     4801 caaattattg ataaccattt aaccttcctt tctatgcaga tataatctat tataacataa
     4861 atcgcttaca aatcgtgtgg gcataaaaac acagtctgca aaaaaagccc tgataggcat
     4921 gcctattaga gcctcgaaaa gaaaaatatt gaatcaatct ggtcgtacct cttggatcgg
     4981 tttgacacga ccgccgattc ttttagcaaa agcttcggat tcttttttgt ctttgaaaat
     5041 gagcagattg tctaagtttt tttcccgagt gcgacagcca gttttatcga tgtaaccgtg
     5101 cattacttta acgacataca tggggatcac ctgctttttt cttttcatta tacgaactac
     5161 tcgacacaat acaagaaaaa tgcttagacg gtctttatta agaatttaca tcatccaaag
     5221 aagcgtacgc ttggaataac tgttctaact ccggattgac tttgctcagc ttcactaatc
     5281 gttgcgaatc agaaagtaaa aaacagtgtt tactactgcc agtagttttt aattttttgt
     5341 cttcagttcc cacaatttcg tagctaaaat ccaaccgtgt tcccgtatac ttttggatag
     5401 ttgggtggat gctcactttt tctccgtaat ggatcatttc tttatagctg caatttactt
     5461 ccagaacggg gatgatgatc ccagattctt cgattttttt atagggaaac cctagatgta
     5521 ccaataaggc tacccgagcc tcttcaaacc agcggatata attgctgtga tgaataatcc
     5581 ccatttgatc tgtttcataa taaaaaggtt ctcgcacata acctgtaaat ttctccatga
     5641 ctcatgcctc gttttccatt gattttttac aaaaataatt catccacttc ggaagtgctt
     5701 cgctgatttg tgtcggcaaa acaatatatt gttcttttgc cagctggtct ccaatgaaac
     5761 tatgaagata aacagctgct gcaatcgttt ggtcattttg aggaaactgg gctaaaaatc
     5821 ctgtgatcat cccagctaaa gtgtcaccca tgccgcctgt tgccatagca gcagaaccta
     5881 aaggattggt gtagaagtgc tggtgaccat aaatttttgt ctcatgactt ttcaaaacga
     5941 gcacagcacc gatttcctgt tgcttctttt tgttttcttt atcccgttgt tcttgtatcg
     6001 gtagagaact caaccgttgc cactccattt gatgaggtgt aagtacagtt tgttcgggat
     6061 agggtagcaa aagatgatta gcaacaagta aacttaaagc agagccatca atcaccagcc
     6121 attgttcttt tgattggttt tctagtactt gtttgaaacg ttccaaactc ttagaagaag
     6181 tacccaatcc gggaccaatc aagagtacat ctgcttgctt tattacggaa agaaactggt
     6241 cagtatccgt ccaatctaca atcattgctt ctggcagatg actgtgaaga gcactccgat
     6301 ttttttcatc agttactaca gtcgtgagac ccgctccact gtgtacggca gctaaagcac
     6361 tcataatgat tgctccgcca tattgttcat tgccgccaat caaaacgata cgtccaaatg
     6421 ttcctttatg tgaaatcttt ggacgaggtg tgatgactct tttcactagt tgttcgttta
     6481 tcagcatcga aaaacctcct tttactcatt atagcgtata ttttttttag agaaaaacag
     6541 ataagaaaag gaattgtcag attttttgaa ctattttttt ctgtaatttg tggtatcatg
     6601 aacacgaaga cttaaataag tggaggatta actttatgac aattgattgg caaaaagaag
     6661 tagaagcacg taaagaagac ttgcttgaag acttgaaaaa tcttttacgt gtcaacagcg
     6721 agcgcgatga ttcaaaagta acaccagatg caccatttgg acctggacct cgcgacgcat
     6781 tgaaacatat gttagcttat ggcgaacgtg atggatttaa agtaaaaaat gtcgataact
     6841 atgcagggca tattgatctt ggcgaaggag acgaaacgct agggatattt ggacacatgg
     6901 atgtcgttcc tgcaggagat ggctgggaca cagatcctta tgaaccagta atcaaggatg
     6961 gaaagatctt tgcacgcgga tcaagcgatg acaaaggtcc aagtatggca gcttactatg
     7021 cgatgaagat cattaaagaa cttgacttga aactatcaaa aaaagttcgc ttcgtagtcg
     7081 gcagcgatga agaaagcggc tggggcgata tggcttatta tttcgaacat gaagaagaac
     7141 cagattttgg tttctcacca gatgctgaat tcccaatcat caatggtgaa aaaggaaatg
     7201 tttctttagc tttacgcttc aaaggcgaca atgcaggaga ttatgtcttg aaatcttttg
     7261 tttccggact tcgcgaaaat atggtgccag gaacagcaac tgcagcactt caagtaccaa
     7321 gtgcagatgc agctatcgcg atggaagaag cattttacca attcatcgaa gcaaatccag
     7381 taagcggaac gattgaagcg gacaatacgt atgtaaaaat cgaattagtt ggtaaaggcg
     7441 cacatggtgc aagcccacaa tctggtatca atgcaggttc tttcttggca ttgttcttag
     7501 ataactatga attcctagga tctgctaaac aatttatcca tgtagcagct gcttatgtcc
     7561 atgaagattt ttatggagaa aaactaggag tcgcttatga agatgaaaaa atgggcaaat
     7621 tgacaatgaa tgctggacta tttgcttttg aagaaaatgg tacagaggaa gcgaacttca
     7681 tcaatatgaa tttccgtttt ccaaaaggtg taacagtaga cggattgcaa agtgacatcg
     7741 aacaaacagt ggggcaagaa ggcgcaacag ttactcgagg tgctcgcgta atggaaccac
     7801 attacgtacc aatggatgat ccattggtgg ctacattatt gcaagtctat gaagaccaca
     7861 ctggagaaaa aggctacgaa caaatcatcg gtggcggaac ttacggacgt ttgttgaaac
     7921 gtggcgttgc ttatggagcg atgttcccag gatatactga tacaatgcat caagcaaacg
     7981 aatttatgag tcttgatgac ttgttcagag caacagcaat ctatgcagat gccatctatc
     8041 gtttggcgaa ataaaaatac taaaaaa
//
LOCUS       DACGLE010000099         7964 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0534
            SAMEA3304449-rid9560453.denovo.105, whole genome shotgun sequence.
ACCESSION   DACGLE010000099 DACGLE010000000
VERSION     DACGLE010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304449
            Sequence Read Archive: ERR985064
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7964)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7964)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702225.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702225.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 20:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,045
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 27 of 67
            Pseudo Genes (incomplete)         :: 25 of 67
            Pseudo Genes (internal stop)      :: 19 of 67
            Pseudo Genes (multiple problems)  :: 4 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7964
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304449-rid9560453.denovo.105"
                     /strain="VREN0534"
                     /db_xref="taxon:1352"
     gene            40..1341
                     /locus_tag="IXT20_002517"
     CDS             40..1341
                     /locus_tag="IXT20_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005227456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ4860934.1"
                     /translation="MENSAFIMKLNDILSPIAAKIGNQRHLKAISSGMMFGLPFIVIG
                     SFFLIFANPPINIDKYNPDTANIFMKFMASWKDFAVANYDLITAPYNLTMGIIGFISV
                     FGIAFSLANEYKINPSMNGMVASVIYLMVCTPVEEGAINLNFLGTNGLFVAIIIGLLV
                     VEINRLFEVKNIKLKLPDTVPPMVATFINMLVPLLTNIFIFYGINLIFLAATNKIFPE
                     AVMEVLTPATNIAGSLGGFLLIVTLGNILWLFGINGSSIIFPIVFTLGMAQTGLNAEQ
                     LANGETMTHLMNLQMFRISVLGGAGGTLGLVLLMMRSKVPEYRTIGKLSLVPGICSIN
                     EPVIFGLPIVFNPILAIPFLITPIISLLLTYFAQQLGWIGIGFIVDPSFTPFFAQAYL
                     SSMDWRNILFCLLLIVISIFIYYPFFKVMESNKTKLVDEKL"
     gene            complement(1505..1627)
                     /locus_tag="IXT20_002518"
                     /pseudo
     CDS             complement(1505..1627)
                     /locus_tag="IXT20_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749140.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2812 domain-containing protein"
     gene            1740..2930
                     /locus_tag="IXT20_002519"
     CDS             1740..2930
                     /locus_tag="IXT20_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAQ4860935.1"
                     /translation="MNYFTTSLMKNLFSNDNNLPAFEQAVTELFRSELEKSLNEILAY
                     ELTDFLDYEPYARTNNENSRNGSYQRKFDTKYGTITLNIPRDRMGEFFTSLLPKYRRR
                     DLFTETTILDLFEEGMSHSEICSMIQKLCGARYSKQTISNITDKALECVDSFKNRILN
                     KEYAIVYLDGTSMPLRRDTVSREMVHIALGITVEGTKEILGYMIAPNESAEIWEELLK
                     DLKSRGVERISLFCTDGLSGMENVIQDIFPGSKIQRCLVHIQRNICAKVRVSDRKEVA
                     NEFKEVYRSKSKEEALTSLKKFIDRWKHKYPSMVKSLSNNDYLFTFYDYPESVRHTIY
                     STNLIEGNNKQLKRSFKKKEQFPTEKSEEKYLVIQFNRYNEKNMNHVHRGFGQTTRED
                     WFKS"
     gene            3099..3521
                     /locus_tag="IXT20_002520"
     CDS             3099..3521
                     /locus_tag="IXT20_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4860936.1"
                     /translation="MKNKFILSLGILTIGISVTGTTIPTFADSTNEPTTVVDNNQELT
                     PLDRNGIAIGNPTNDLTVTENYLIVSPLLARAASKTIRVTGTMIFDKVGWPSRPPQSR
                     NWKEKRNGYWYYGKLYLQSYKSAGNNWIATYSGTLTRK"
     gene            3531..3734
                     /locus_tag="IXT20_002521"
     CDS             3531..3734
                     /locus_tag="IXT20_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4860937.1"
                     /translation="MPEFVNTPNFYQDSDILSKINLPKEESALQVQRTLQETQKIQKS
                     ISNLEDSEEKRKKRDQKTYQKEK"
     gene            4054..4689
                     /locus_tag="IXT20_002522"
     CDS             4054..4689
                     /locus_tag="IXT20_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAQ4860938.1"
                     /translation="MQKVGKNLRVAYIRVSSIDQNEQRQVEEMKKFGAERIFIEKQSG
                     ATITHRPVFQEVLDYVRDQDIFIVEAIDRLGRNYDEIIASVNYLKKKNVQLIITSLPI
                     MAEAIGNPLLDKFIKDLIIQILAMIAEQERTESKRRQAQGIKIAKANGVYKGRPKLYS
                     ANAKDPQRRLVYKNIVEDLKKGVAIAKIAKDYNVTRQTVYRIKKDSTVNHE"
     gene            complement(4713..5063)
                     /locus_tag="IXT20_002523"
     CDS             complement(4713..5063)
                     /locus_tag="IXT20_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016610020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HAQ4860939.1"
                     /translation="MVRKPRQGDILLLNTAPRSGHEQTGKRPYIVLSHDIIADYSNVV
                     TVAPISSTTRNYPLYVNINSEYEMKTTGKVLLDQLTTIDYEARECIFLEKAHDSLVEE
                     LLMKVRTVFQKVSK"
     gene            complement(5057..5320)
                     /locus_tag="IXT20_002524"
     CDS             complement(5057..5320)
                     /locus_tag="IXT20_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PbsX family transcriptional regulator"
                     /protein_id="HAQ4860940.1"
                     /translation="MEMLPIKKWGNSNGLRLPKYIMEYLGIHTEDKVKIIQEEVNGQK
                     RLIIEAANIENELTIEQLFENYTDERNHVTIQNLGEAVGNEKW"
     gene            complement(5661..5840)
                     /locus_tag="IXT20_002525"
     CDS             complement(5661..5840)
                     /locus_tag="IXT20_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4860941.1"
                     /translation="MPIAIGNKRLPVTLDEKRQKELQKLKQKYGKSESKIMCIALDLL
                     IAQEKAGFDVPALKK"
     gene            complement(6285..6722)
                     /locus_tag="IXT20_002526"
     CDS             complement(6285..6722)
                     /locus_tag="IXT20_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4860942.1"
                     /translation="MAQNYYDEFVKLPLDKMAQKMEDMTFLYNETRVPKKHYKEKLSV
                     AVEEMIESGVEMNLIATYYRTLEELKKQNAKWFFQALLCLEVGVKPSTIKPSEYQALE
                     LTYAKFIETKKAKTVSSEWLDYFENINKYGAYYTMKKEDNENE"
     gene            complement(6738..6989)
                     /locus_tag="IXT20_002527"
     CDS             complement(6738..6989)
                     /locus_tag="IXT20_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4860943.1"
                     /translation="MKTQHKLLTVKEVVQILVDKEVISEKVAEALLIPRYWTIETPLV
                     EDAPTMLFEDLVKIAGYELHYQENMETYENEYWLTKLEN"
     gene            complement(6989..7195)
                     /locus_tag="IXT20_002528"
     CDS             complement(6989..7195)
                     /locus_tag="IXT20_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrA"
                     /protein_id="HAQ4860944.1"
                     /translation="MRKKFDYWGVPFSELFPNYHAPHTVECDCGERAKCIKSYRLYQC
                     QTCGKKYTLSYGDYVLIDEKGKKH"
     gene            complement(7638..7898)
                     /locus_tag="IXT20_002529"
     CDS             complement(7638..7898)
                     /locus_tag="IXT20_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4860945.1"
                     /translation="MDKYVGLLDKIRVIKTQPLLVRFTLQTIHESINCVVADIEIIDK
                     LLIMDDGKYNIAVTGHFNKRNQLVIASMYVRNPDHFTRSMGI"
BASE COUNT         2574 a         1429 c         1326 g         2635 t
ORIGIN      
        1 aactattaga caaatgatat tttacgagga ggaagttata tggaaaattc tgcgtttatc
       61 atgaaactga atgacatact tagccccatc gcagccaaga taggtaatca gcgccaccta
      121 aaagctatat ccagcggtat gatgtttggc ctacccttta ttgttatcgg ttcttttttt
      181 ctgatttttg caaatccacc catcaatatt gataaataca atccagatac agctaatatt
      241 ttcatgaaat ttatggccag ctggaaagat tttgccgtag caaattatga tctcatcaca
      301 gccccttata atttgacaat gggaatcatc ggattcatta gtgtttttgg cattgcattt
      361 tcccttgcca atgaatacaa aattaatcct tccatgaatg gcatggttgc atctgtcatt
      421 tatctaatgg tttgtacacc agtggaagaa ggtgccatca atcttaattt ccttgggaca
      481 aacggattgt tcgtcgcaat tatcatcggc ttgctagtag tggaaatcaa ccgactattt
      541 gaagtaaaaa atattaagtt gaaactccca gatacagttc cacccatggt agcaaccttt
      601 atcaatatgc tcgttccact gctgacaaat atattcatct tttatggcat caatctgatt
      661 ttcttggcag caacaaacaa aatcttccct gaagcggtga tggaagtcct gactccagca
      721 acgaacatcg ctggcagtct aggtggtttc ttattgatcg tgactttggg taacatccta
      781 tggctatttg gtatcaatgg ctcgtcaatt attttcccta tcgtctttac attgggaatg
      841 gctcaaactg gtttgaatgc tgaacaactg gcaaatggtg aaaccatgac acacttaatg
      901 aacttacaaa tgttccgtat ttctgtactt ggtggagccg gtggtacttt gggtttggtt
      961 ttattgatga tgcgaagcaa agtacccgaa tatcgcacaa ttggaaaatt gtcattggtt
     1021 cctggaatct gttccatcaa cgaaccagtg attttcggat tacctatcgt ttttaaccct
     1081 attttggcaa ttccattttt gattacgccc atcatttcat tattgctgac ctactttgct
     1141 cagcaactag gatggattgg gattggattt atcgtcgatc cttccttcac accttttttt
     1201 gcacaagctt atctttcaag tatggactgg cgcaatattc tcttttgcct actgttgatc
     1261 gtcatcagca tcttcatcta ctatccattt tttaaagtca tggagagcaa taaaacaaaa
     1321 ttagtagatg aaaaactata gtcgtcattc ttaaccttgt aacgaaaata tggttctata
     1381 ctatttagtg ttgatgggaa atcttagggt ttcccatcaa cctatttttt ataaaaaagt
     1441 atactctggt gtcccacaac aaataaagga agcgcccttg ttcaaaaggc tacttccttt
     1501 atttttattt tgctttaaat tttgagtcat catttataag cgaacgtaag agataataga
     1561 cacctaaaaa tagaaagaaa aataaaggta atacacgtag taaaacaaat ggagtttcaa
     1621 aaataaatgg atagcataaa atatcttgtg taaattcgtt tttctagaaa tgtagaaaaa
     1681 aaggtatatc cttagtaaaa ttagttcgac caaaaacaat ttacgaaagg aatataccca
     1741 tgaactattt tactacatct ttaatgaaaa acctattttc taatgacaac aatcttccag
     1801 cttttgaaca ggctgtaacc gaactgtttc gttcagagtt ggagaaatct ctcaatgaaa
     1861 tactcgcata tgagttgacg gattttcttg attatgaacc atacgctaga accaataacg
     1921 aaaattctag aaatggttcc tatcaaagaa agtttgatac aaaatatgga actattacac
     1981 tcaatattcc aagagataga atgggagaat ttttcacttc tcttctgcct aaatacagaa
     2041 gacgagatct atttaccgaa acaacgattt tggatttatt tgaagaagga atgagccatt
     2101 cagaaatttg ttccatgatc caaaaattat gtggagctcg ctattctaaa cagaccatat
     2161 ctaatattac ggacaaagct ctggaatgtg tagattcctt caaaaacaga atattgaaca
     2221 aagagtatgc cattgtatat cttgatggaa catccatgcc tttacgccgt gatactgtat
     2281 ccagggaaat ggtccatata gcactaggaa tcacagtaga aggtacaaaa gaaattcttg
     2341 gctatatgat tgcaccaaac gagtccgctg agatatggga agaacttctg aaagacctta
     2401 aatcaagagg ggttgaacgt atttctttat tttgtacaga cggattgtct gggatggaaa
     2461 atgtaataca ggacattttc ccgggctcta aaattcaaag atgcctggtc catattcaaa
     2521 gaaatatatg tgcaaaagta agagtcagtg acagaaaaga agtggccaat gaatttaaag
     2581 aggtatatcg gtccaaatca aaagaagaag ctttaacttc tctaaagaaa ttcatcgata
     2641 ggtggaaaca caaatatcca tcaatggtga aaagtctcag caacaatgac tatctgttta
     2701 cattctatga ttatccagaa agtgttaggc atacgattta ttcaacaaat ctgattgaag
     2761 gaaacaataa acaattaaaa cgaagtttca aaaagaaaga acaatttcca acggaaaaat
     2821 ctgaagagaa ataccttgtg attcagttca atcgttacaa cgagaaaaac atgaatcacg
     2881 tacatagagg ttttggacaa acaaccagag aagattggtt caagagctaa tctgctttac
     2941 caaggatata cttttacaca aaattattga cgctcccaaa tttaaacgat tatgacatta
     3001 aaaatatatt ttatgaattt tctagtatat aacttgattt atctgttaag atttattaag
     3061 aaaacgtttt cttatttttg atttcgaggt gatagacaat gaaaaataag tttattttat
     3121 ctttaggtat tttaactatt ggaattagtg taactggaac tacaattcct acatttgctg
     3181 atagtacaaa tgaaccaact actgtagtag ataataacca agaattaact cctttagata
     3241 ggaacggaat agccattggt aacccaacta acgacttaac tgttactgaa aattatttaa
     3301 tagtttctcc attacttgct cgagcagctt ctaaaacgat acgagtgaca ggaacgatga
     3361 tttttgataa agttggttgg ccttctagac ctcctcaatc acggaattgg aaagaaaaaa
     3421 gaaatggtta ttggtactat ggaaaattgt atttacaaag ttataaatca gctggaaaca
     3481 attggattgc gacttattca ggaaccttaa ctagaaagta gggatttttt atgccagaat
     3541 ttgtgaacac accaaatttt tatcaagata gcgatattct aagtaaaatt aatttaccga
     3601 aagaagaatc agctttacaa gttcagcgaa ctttacagga gacacaaaaa attcaaaaat
     3661 caatttctaa tctagaagat tctgaagaaa aaagaaaaaa aagagaccaa aaaacatatc
     3721 aaaaagaaaa ataatgattt taaaaaaaga gtgtctagtc gacattcttt ttttctaatt
     3781 gaaagtgttc ataaaatcac ccatttctgg acactttctg tcatttttat atttaattgg
     3841 cacagttttt tctaattatt tgtcatattc atagtcaact atagctacaa aagcaactaa
     3901 ttttggagat agattgagat atttaaaaat ctatcatgaa aaattagttg cttttgtcac
     3961 ttagcgctga aaatttatgc catttatcta gaaaaatgac aaacattgaa aaatctggtt
     4021 ttctcatgta tgattagggt gtgttttaaa tagttgcaga aagtaggtaa aaatttgaga
     4081 gttgcataca tacgtgtctc atcgatcgat caaaatgaac aacgccaagt tgaagaaatg
     4141 aaaaaattcg gtgcagagag aattttcatc gagaaacaat ctggcgctac aattactcat
     4201 cgacctgttt tccaagaagt attggattac gtccgagatc aagatatttt tattgtggaa
     4261 gctattgatc gacttggaag aaattacgac gagatcatcg cttcagtcaa ttacttgaag
     4321 aagaaaaatg tgcagctgat cattacaagt ctaccgatca tggccgaagc aattggcaat
     4381 cctctcctgg ataaattcat taaagatttg atcattcaaa ttttggccat gatcgctgaa
     4441 caagaacgta ccgaatctaa acgacgtcag gcacaaggca ttaagattgc gaaagcgaac
     4501 ggtgtttata aaggacgtcc taagctatac agtgccaatg caaaagatcc ccaacgtcgt
     4561 cttgtttata aaaacatcgt tgaggatcta aaaaaaggtg ttgcgatcgc taagattgca
     4621 aaagactaca atgttacgag acaaaccgta tatagaataa aaaaagacag cacggtcaat
     4681 cacgaatagt tgatcatgct gctgtttcta ttttatttgc ttactttttg aaaaactgtt
     4741 ctaactttca tcaataactc ctcaactaaa ctatcatgcg ctttttccaa aaaaatacac
     4801 tctctggctt cataatcaat tgtcgttagt tgatctaata atacttttcc tgttgtcttc
     4861 atttcatatt ctgagtttat atttacatat agcggatagt ttctagttgt actagaaatt
     4921 ggagcaactg taacaacgtt actataatcc gcaataatat catgacttaa aacaatataa
     4981 ggccttttac ctgtctgttc gtgtccagat cgaggtgctg tattcaataa tagaatatcg
     5041 ccttgtctag gttttctcac catttttcat tacctactgc ttcacctaaa ttttgaatcg
     5101 tcacatgatt tctttcatca gtgtaatttt caaataactg ttcaattgtt aattcatttt
     5161 ctatattagc cgcttcgata attagccgct tctgaccgtt tacttcttct tgtattatct
     5221 tcactttgtc ttctgtatga attccaagat attccataat atactttggc aatctaaggc
     5281 cattactatt tccccatttt tttataggta acatttccat agtagacacc tctttctgta
     5341 tatacatagt ataacataca taagaaaaaa cgtatatact taattatcct tttctattaa
     5401 attagattaa cattccatga atccataaac tatgttaaca tttatatgaa aatagtcgaa
     5461 aggaaatcat aatatgttta agaaaacaaa atctttagaa tcaggtaaga aaaaaattga
     5521 tctaaaaata atctcttctg gatactttaa agatttcata gttattgata caaaatggaa
     5581 taaaaaggat atagaaaata ttctaaatag taggaacaat taagtgctat cacttttgtt
     5641 tcttcaaatg aaaagtgacg tcactttttg agtgctggta catcaaatcc tgctttttct
     5701 tgcgcgatca atagatctaa cgcaatacac ataatcttag attcactttt tccgtacttc
     5761 tgctttagtt tctgcaattc tttttgtctc ttttcatcca acgtaacggg taaccgttta
     5821 tttcctattg caattggcat tagtactctc tcctttcatc gaaataaccc tatgaaaaat
     5881 atggctgcaa tcaacttgaa tattagactc acaagggcta acacaaagcg ccaaaagatt
     5941 aaacaaaaca ccagtattac caaaaatact acgaagttta aatgcacagc tgcactatgt
     6001 cgcataatta tttcgccaac aaatagtact aacaataagg gaataatctt gttcaaatgg
     6061 atcaccttct ttctcttgag ttatcgggct ttgcccgttt atttcgcatc acctaagcgg
     6121 cgggggcggt tggagcgatt ggtgcgaaat aagaaccatt cccttagtta ctaggctttt
     6181 tttgtcctta tttcacacat tctcactttt cggtataaat gattattgac ctttttttct
     6241 ttcaaatcgt tgatatggaa tccgttgtta gttgataaat caccttattc attttcgttg
     6301 tcttcctttt tcatggtgta atatgcgcca tacttattga tattttcaaa ataatctaac
     6361 cattcacttg aaacagtctt ggcttttttc gtttcgataa atttcgcata cgttagttct
     6421 aaagcttggt attcagaagg tttgattgtg cttggtttga cgcctacttc caagcatagt
     6481 aaggcttgga aaaaccattt tgcattttgc tttttcagtt cttccagtgt tcgatagtac
     6541 gtcgcaatga gattcatttc gacacctgat tcaatcattt cttccacagc taccgacaat
     6601 ttttctttat aatgtttctt tggaacacgt gtttcgttgt ataggaacgt catatcctcc
     6661 attttttgtg ccattttatc caaaggtaat ttgacaaatt cgtcatagta attttgagcc
     6721 atattttttt ctccttttta gttctctaat ttggttaacc aatattcgtt ttcataagtt
     6781 tccatgtttt cttgataatg cagttcatat cctgcaatct tcactaggtc ttcaaatagc
     6841 atagtcggtg catcctctac taaaggggtt tctatcgtcc agtaccgcgg aataagtaaa
     6901 gcttctgcta ctttttcaga aatgacttct ttgtccacca aaatttgaac gacttctttt
     6961 acagtcaata atttgtgttg tgttttcatc agtgtttctt tcctttctca tcaatcaaaa
     7021 cataatcgcc ataagacaag gtatattttt tcccgcatgt ttgacattga tacaaacgat
     7081 agcttttgat acatttcgct ctttctccac aatcgcattc gacagtatgt ggtgcatgat
     7141 agtttgggaa taattcgctg aagggaacac cccaataatc aaatttcttt cgcatgattg
     7201 attctgcctc ctcttaggaa agggtttact tcctttcgtt agttctttct ttattgattt
     7261 gttggccaga atgaatcaac gaaaagcgac aaggacaagg gcgcgcagcg tgccatttac
     7321 ccttgtcctt gtcgcttttt ggtgataaaa ggcaacttgt tgccggtctg gcaaatcaat
     7381 aaagagatag cgaacaggaa cgaacctttt ggtgagagga agtttttcct attttgtctt
     7441 gctttgcaag accccttatc ttagtgaacg tgcaacgttc acttcctttc ttctcgaacg
     7501 gcggtggcgg ttggagtgag agaaaggttt ttcaattgag aaggcgcttg tgccttccta
     7561 aacaggcctt ttcctgttcc cgtttctatg taaaacaaaa agagctgcaa tcctcattta
     7621 ggactacaac tcttctttta tatgcccatc gatcgcgtaa aatgatcggg atttctaaca
     7681 tacatgctag caataactag ctgatttcgt ttattgaagt gacctgtgac ggcaatattg
     7741 tattttccat catccataat tagcagttta tcgataattt caatatctgc aactacacaa
     7801 ttaatcgatt catgaattgt ttgtaaagta aagcgaacca ataatggttg tgtttttatt
     7861 actcggattt tatcaagcaa accaacatat ttgtccatca caatcacctc gctaaaatta
     7921 tagcaaaaac aaccaaaatt gagaattttt attcgatttt agat
//
LOCUS       DACGLE010000100         7760 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0534
            SAMEA3304449-rid9560453.denovo.106, whole genome shotgun sequence.
ACCESSION   DACGLE010000100 DACGLE010000000
VERSION     DACGLE010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304449
            Sequence Read Archive: ERR985064
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7760)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7760)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702225.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702225.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 20:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,045
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 27 of 67
            Pseudo Genes (incomplete)         :: 25 of 67
            Pseudo Genes (internal stop)      :: 19 of 67
            Pseudo Genes (multiple problems)  :: 4 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7760
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304449-rid9560453.denovo.106"
                     /strain="VREN0534"
                     /db_xref="taxon:1352"
     gene            complement(552..1898)
                     /gene="gor"
                     /locus_tag="IXT20_002530"
     CDS             complement(552..1898)
                     /gene="gor"
                     /locus_tag="IXT20_002530"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HAQ4860946.1"
                     /translation="MQIYDYIVIGGGSGGIASANRAGMHGAKVLLIEANELGGTCVNV
                     GCVPKKVMWQASTILETIERDASSYGIQADLKNVDFAGLVERREKYIDFLHGAYQRGL
                     DSNHVEAIKGYATFIDSQTIEVNGETYRAPKILIATGGRASKMTIPGGEYAIDSNGFF
                     ELTELPKRAIVLGAGYIAAELSGVFRGLGSEVMWAYRKERPLRTFDKMLSDNLIQMYQ
                     EAGIKTYAHHIAKEITKNNDEYTVLFENGETLTGDCVLFAGGRVPNTEKLGLENTNVE
                     LDKKGFIKVDKYQNTTDEHIFAVGDVIGKLDLTPVAIAAGRRLSERLFNGKKDSYLDY
                     KLVPTVVFTHPPIATIGLTEEEALEKYGENELKVYRSRFTPMYFALNDYRQKCEMKLI
                     CVGKEERIVGLHAIGVGVDEMLQGFAVAIKMGATKEDFDNTVAIHPTGAEEFVTMR"
     gene            complement(2032..3273)
                     /locus_tag="IXT20_002531"
     CDS             complement(2032..3273)
                     /locus_tag="IXT20_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HAQ4860947.1"
                     /translation="MTVKRKLKRPEILAPAGTLEKLKTAIHYGADAVYIGGNAYGLRS
                     RAGNFTYEEMAEGVAFAKEHDAKVYVAANMVTHEGNEDGAGEFFRSLRDVGISAVIVS
                     DPALIEICATEAPGLPIHLSTQASATNFETLEFWKEEGLERVVLAREVSMEEVAEIRK
                     NTDTEIEAFIHGAMCISYSGRCTLSNHMSMRDANRGGCSQSCRWKYGLFDMPFGQERN
                     SLTDTGEVEEEFSMSAVDMSMIEHIPELIENGVDSFKIEGRMKSIHYVSTVSNVYKAA
                     VDSYMEDPENYVCKQEWIDELWKVAQRELATGFYYNTPTENEQLFGERRKIPVYKFVG
                     EVLSYDEATQIATIRQRNLFSVGDEIEFYGPGFSHFSQTVEVMHNEEGESIDRAPNPM
                     MILTMPVAQPVKPGDMIRKRK"
     gene            complement(3291..4214)
                     /locus_tag="IXT20_002532"
     CDS             complement(3291..4214)
                     /locus_tag="IXT20_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HAQ4860948.1"
                     /translation="MIELITTVETIEQAEQLLAAGADTLYFGEEQFGLRLPASFTREE
                     QRQLVELAHKYGKKANIAVNGIIHPEKMKNVPEYLAFLKEINVDVITVGDTGIIYTMK
                     KNPELRIPYIYDAETLVTSARQINFWAKKGAVGAVLAREVPFEEMKAMEEKLDIPVET
                     LVYGATCIHQSKRPLLQNYYNYTKQDEQKDRERGLFISEPKKEETHYSIYEDSHGTHI
                     FASNDLNLSNELNELYQHHYCTWKLDGLFTPGEKFVNITRLFAEAKKQILEGSWDQEK
                     AEKMTSEISKLHPENRELDTGFFYIDPSAIK"
     gene            complement(4435..6927)
                     /locus_tag="IXT20_002533"
     CDS             complement(4435..6927)
                     /locus_tag="IXT20_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HAQ4860949.1"
                     /translation="MEELFTERAQAVLEIAQEEAKRLKHQSVSSEHVLLALVVEQNGI
                     AGKVLREMDLNETEIYEEIEHLIGYGGIRSYPKGVFLPYSPRMKQVFALASSEVKKTG
                     SQKVGTEHILMSLLKDESIMATRIMINLGISLSKARQVLKQKMGLAGDSAGKGMNRRR
                     NVNVQDKRQTPQGTPTLDSLARDLTKLAKENKLDPVVGRSREVKRLIQILSRRTKNNP
                     VLVGEPGVGKTAIAEGLAQKIILGEVPEDMQEKRLMMLDMGSLVAGTKYRGEFEDRMK
                     KVIDEIYNDGQVILFIDELHTLIGAGGAEGAIDASNILKPALARGELQTIGATTLDEY
                     QKYIEKDSALERRFARIQVDEPTPEEAEVILQGLRTRYEEHHGVEITDEAVRAAVNLS
                     VRYITSRQLPDKAIDLIDESAAKVRLDQTDHLTKSTVIKLEIDELVQEKEAAIQKQDF
                     ENAAQLRRQEKALRKKLQKVSAIEAKQEQGYSDRVTEEDVATVVSEWTGVPLQQLEKK
                     ESERLLELEGLLHERVVGQDEAVKAVSRAIRRARSGLKDPDRPIGSFMFLGPTGVGKT
                     ELAKALSEVMFGSEDALIRVDMSEFMEKYSTSRLIGSPPGYVGYDEGGQLTEKIRQKP
                     YSVILLDEVEKAHPDVFNLLLQVLDDGHLTDSKGRKVDFRNTIMIMTSNIGATQIREE
                     KNVGFNVQDVTKDHKAMQKRILEELKKAFRPEFLNRIDETVVFHSLSKDEIHTIVQIM
                     SKAIIKRLKEQDIQVKITPSAIDVIGKAGFDPEYGARPIRRALQKEIEDRLSEALLGG
                     EIRLGDHVTVGASKGKITLNVRGPKKETVGSL"
     gene            complement(6934..7401)
                     /locus_tag="IXT20_002534"
     CDS             complement(6934..7401)
                     /locus_tag="IXT20_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HAQ4860950.1"
                     /translation="MAHQNTSDLIESYIKKILENSEMIEIRRIEMADLFNCVPSQINY
                     VINTRFTIQRGYLVESKRGGGGYIRIAKVRISDKKQLLEQINQLFDDTISEKNAFAII
                     QKLYEDQIITKKEGNLMLSAIAKNTLNFNEYEDHTRARILRAFLERLSYEDGK"
     gene            complement(7537..7610)
                     /locus_tag="IXT20_002535"
     tRNA            complement(7537..7610)
                     /locus_tag="IXT20_002535"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7574..7576),aa:Arg,seq:acg)
     gene            complement(7668..7741)
                     /locus_tag="IXT20_002536"
     tRNA            complement(7668..7741)
                     /locus_tag="IXT20_002536"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7705..7707),aa:Pro,seq:tgg)
BASE COUNT         2161 a         1691 c         1247 g         2661 t
ORIGIN      
        1 aaatcagtgt tttgtcctga gtattagaga aacaaatgga aaaatagttc ctcatatttt
       61 gccgtttttt gagctaatac cgaaggacag ctgattgaac actatttgtt cggaaaaagt
      121 gactaactat gacacaattt ttttcacagt cccttttttc acttgatttg attcgtacca
      181 gcgaacttga cgagaacttt cttggtttgg taactatatg gtattctttt aacatcctcg
      241 tttgcttctt ctaatttatt tatacaaagt cacacttttt taaacatctg ttcttcgata
      301 catttctatt tcttttaaac atcgatacca atattttgaa cttaagagtg cataccaaat
      361 ggattcattt actcttcgtc agttacgaag ataatcaaaa tctccaactt ccctacttat
      421 ttcgttttga gaattatcga agcaatctta cataaataac taaaaaacaa aaaaccagaa
      481 aatcagtata ctgattttct ggttttacat ggtttgttca ataaaaagct gttggaactg
      541 ttaatattaa attatcgcat cgttacgaat tcttccgctc ctgttggatg gatggctact
      601 gtattgtcaa agtcttcttt agttgcaccc atcttgatag ccacggcaaa accttgcagc
      661 atctcatcga caccaacacc aatagcatgt aagccgacga ttctttcttc ttttccgaca
      721 cagatcaatt tcatttcgca tttttggcga taatcattta atgcaaagta cattggggta
      781 aagcgtgaac gataaacttt caattcattt tcgccatatt tttccaatgc ttcttcttct
      841 gtcaaaccaa ttgttgcaat tggtggatgc gtaaagacga ctgtcggaac aagtttatag
      901 tcaagataag aatctttctt tccattaaat aaacgttctg ataaacgtct accagctgcg
      961 attgcaacag gagtcagatc taatttaccg atcacatcac caacagcaaa aatatgttca
     1021 tccgttgtat tttggtattt atctactttg ataaaccctt ttttgtccaa ttccacattt
     1081 gtattttcca gacctaattt ttcggtattc ggtacacgtc caccggcaaa aagtacacaa
     1141 tcaccagtta aggtttcacc attttcaaat agcaccgtat attcgtcatt gtttttagta
     1201 atttctttag caatatgatg tgcgtaagtt tttattccag cttcttggta catttggatc
     1261 aaattgtccg aaagcatttt atcaaacgtt ctaagtggtc gttcttttcg atatgcccac
     1321 atgacttcac tacctaaacc acgaaataca cctgaaagtt cagccgcaat gtatccagca
     1381 cctaaaacga ttgctctctt aggtaactct gttaattcaa aaaatccatt tgaatcaatt
     1441 gcgtattctc caccaggaat ggtcatttta cttgcacggc ctccagtagc gatcaagatt
     1501 ttaggagcac gataggtttc accgtttact tcgatcgttt gactatcgat aaaagtagcg
     1561 taacctttta tagcctcgac atgattgcta tctaatcctc tttgataagc accgtgtaga
     1621 aaatcaatat atttttctcg tctttctact agaccggcga aatcaacatt ttttaaatct
     1681 gcttggatac cgtaacttga ggcatctcgt tcaattgttt ccaaaatcgt acttgcttgc
     1741 cacataactt ttttaggtac acaaccgacg ttgacacatg ttccgcccaa ttcattcgct
     1801 tcaatcagca aaacttttgc gccatgcatc cctgctctgt ttgctgaagc aataccgcca
     1861 gagccgccac caattacgat gtaatcatat atttgcattt tgttcctcct tattgaagag
     1921 atcaatcaaa acctttattt aacgatcctt tttctacaga aaacaagtgt aacacttttc
     1981 ttgaattttg tatgcgaaga tgcttaaaac agggatatat cagctttttc cttatttacg
     2041 ttttcggatc atatctccag gtttcaccgg ctgtgcgact ggcatagtta aaatcatcat
     2101 tggatttggt gctcggtcaa tggattcgcc ttcttcatta tgcataactt caacggtttg
     2161 actgaaatga ctgaatcctg gaccataaaa ttcgatttca tctcctacag aaaacaagtt
     2221 tctctgacgt atcgtagcaa tctgtgttgc ctcatcataa gataatactt caccgacaaa
     2281 tttatatact gggattttac gacgttcacc gaataattgt tcgttttcag ttggtgtatt
     2341 gtagtaaaaa cctgtagcta attctcgttg tgctactttc cataactcgt cgatccattc
     2401 ttgcttgcag acatagtttt caggatcttc catataactg tctacagccg ctttatagac
     2461 atttgacacc gtagatacat aatggatcga tttcattctt ccttcgatct tgaagctgtc
     2521 tacgccgttt tcaatcaatt ctggaatatg ttcgatcatt gacatatcca ctgcactcat
     2581 cgagaattct tcttccactt cgcctgtatc agttaaactg tttcgttctt gaccaaaagg
     2641 catatcgaac aatccatact tccagcgaca agattgagaa catccgccac ggtttgcatc
     2701 ccgcatcgac atatggttgg acaatgtgca tcgaccagaa taagagatac acatggcacc
     2761 atggataaat gcttcgattt cggtatccgt atttttacga atctctgcca cttcctccat
     2821 tgaaacttca cgagctaaaa cgacacgttc caacccttct tctttccaga attccaatgt
     2881 ctcaaaatta gttgctgaag cttgagtgga taaatggatc ggcagacctg gggcttctgt
     2941 cgcacaaatt tcgatcaaag ctggatcaga aacaatcact gcagaaatac cgacatcacg
     3001 caaactgcgg aaaaattccc ctgctccatc ttcatttcct tcatgggtga ccatattagc
     3061 agcaacgtat accttcgcat catgttcttt tgcaaaagca actccttcag ccatttcttc
     3121 ataagtaaaa ttacctgcac ggcttcttaa accatatgca ttcccgccaa tatatactgc
     3181 atctgctcca taatggatcg cggtttttaa cttttccagt gttcctgctg gcgctaggat
     3241 ttctggtcgt ttcaattttc tttttactgt catttctttc atcatactcc ttatttgata
     3301 gcagaaggat caatataaaa gaatcctgta tctaattcac ggttttcagg atgcaattta
     3361 ctgatttcgc tagtcatttt ttccgctttt tcttgatccc agctaccttc taaaatttgt
     3421 ttttttgctt cggcgaatag tcgagtgatg tttacgaatt tttcgcctgg agtaaataat
     3481 ccgtccaatt tccatgtaca gtaatgatgc tgataaagtt cattcaattc attcgagaga
     3541 ttcagatcat tgctggcaaa aatatgtgtg ccatgactat cttcataaat agaataatgt
     3601 gtctcttctt ttttaggttc cgagataaaa agacctcttt cacgatcttt ttgttcgtct
     3661 tgtttcgtat agttatagta attctgtagc agtggacgtt tggattgatg gatgcatgta
     3721 gcaccataca caagggtttc tacaggaata tcgagttttt cttccatcgc cttcatctct
     3781 tcaaaaggaa cttcacgagc aagtactgct ccaactgcgc cttttttcgc ccagaaattg
     3841 atttgacgtg cgctcgttac taatgtttca gcatcataaa tatacggtat acgcaattca
     3901 ggattttttt tcatcgtata aataatacct gtatcaccaa ctgtgatcac atctacgttt
     3961 atttctttta aaaaagccaa atattcaggt acatttttca ttttttcagg atgaatgatc
     4021 ccatttacag caatattcgc ttttttacca tacttgtgtg ctaattctac caattgtcgc
     4081 tgttcttctc gtgtaaatga agcaggcaga cgcaatccaa attgctcttc accgaaatat
     4141 agtgtatctg cacctgcagc aagcaactgt tctgcttgtt caatagtttc tactgtggtt
     4201 atgagttcga tcataatttt ctcctcctac aacttcataa tagtagcata gctctaaaaa
     4261 ctttccaata cttcttctat ttaaagtcgg atttttgaaa attatttcac aaaaaatcaa
     4321 aataaagttc gatatattct gtccaaatat aaaaagagat tgtgacaaaa atcttgtcac
     4381 aacctcttaa tcaagtctta ctattataaa aattcagcta cacaggccaa gtaactatag
     4441 ggaacctact gtttcctttt taggtccgcg aacattcaat gtgatcttac cttttgaagc
     4501 acctactgtt acgtggtcac ctaaacgaat ttctccgcca agcaaagcct cgctcaatcg
     4561 atcttcaatt tctttttgaa gtgcgcgacg gattggacgt gcaccatatt caggatcaaa
     4621 tccagctttc ccgatcacat cgattgcaga tggtgtgatt ttcacttgaa tatcttgttc
     4681 tttcaatcgt ttgataatcg ctttgctcat gatttggacg attgtatgga tttcatcttt
     4741 actcaatgaa tggaagacga cagtctcatc gattcgattc aagaattcag gtcggaaagc
     4801 tttctttaac tcttctaaga tacgtttttg catggctttg tgatctttag taacatcttg
     4861 gacattgaat ccgacatttt tttcttctcg aatttgtgtc gctccgatat ttgaagtcat
     4921 aatcatgatt gtatttctaa agtccacttt tctaccttta gaatctgtta aatgaccatc
     4981 atccaatact tgcagaagta aattgaaaac gtctggatgt gctttctcca cttcatctaa
     5041 gagaatcact gaatatggtt tttgacgaat tttttctgtc aactgtccac cttcatcata
     5101 ccctacgtat cctggaggag acccgattaa tcggcttgta ctgtattttt ccataaattc
     5161 tgacatatca acacggatca gggcatcttc acttccaaac atgacttctg aaagtgcttt
     5221 ggccaattct gtttttccga caccagttgg tccaaggaac atgaatgaac caattggacg
     5281 atcaggatct ttcaatccgc ttcgtgcacg gcggatcgca cgtgaaactg ctttgacagc
     5341 ctcatcttga ccaacgacac gttcatgcag aagtccttcc agttctaaca agcgttcgct
     5401 ttcttttttc tccaactgct gtagagggac accagtccac tccgatacaa ctgtagcaac
     5461 atcttcttct gttacacgat cagaataacc ttgctcctgt tttgcttcaa ttgcagaaac
     5521 tttttgcagc tttttacgta gtgctttttc ttgtctgcgt aattgagcag cattttcaaa
     5581 gtcttgtttt tggatcgctg cttccttttc ttgtactagt tcatcgattt ctagtttgat
     5641 cacagttgat tttgttaagt gatcggtttg atccaaacgg actttcgctg ctgattcatc
     5701 gatcaaatca attgctttat cgggtagttg acgagaagtg atataacgaa cagaaagatt
     5761 gactgctgct cgtacagctt catctgtgat ctccactcca tggtgctctt catatcttgt
     5821 acgtaatcct tgaagaatca cttctgcttc ttcaggagtt ggctcatcta cttgaatgcg
     5881 agcaaaacgt cgctctaatg cagaatcttt ttcgatatat ttttgatatt catctagggt
     5941 cgttgctccg atcgtttgta attctcctcg agctaacgcc ggtttcaaga tattcgaagc
     6001 atcaattgct ccttctgctc cacctgcacc aattaaggta tgcaattcat caatgaataa
     6061 gattacttga ccatcattat agatttcgtc gatgaccttt ttcattcgat cttcaaattc
     6121 accgcgatat ttcgtaccag caaccaaaga tcccatatca agcatcatca ggcgcttttc
     6181 ttgcatgtct tctggcactt cacctaaaat gattttttga gctaaccctt cagcaatggc
     6241 tgttttgcct acaccaggtt cacccaccag tacagggtta ttctttgtcc gtcggctaag
     6301 aatctggata agccgtttca cttctctcga acgaccaaca actgggtcca gcttgttttc
     6361 ttttgctaat ttcgtcaaat cacgtgctaa tgaatctagc gtaggtgttc cttgcggtgt
     6421 ttgccgttta tcttgtacat ttacatttct acgacgattc attccttttc cagcagaatc
     6481 accagctagc cccatttttt gtttcagaac ttgacgggct tttgaaaggc tgatacctaa
     6541 gttgatcatg attcgagtag ccatgatact ttcatctttt aataagctca tcaaaatatg
     6601 ttctgttcca actttttgag aaccagtttt cttcacttcg ctgctagcta aagcaaaaac
     6661 ttgtttcatt cttggtgaat atggaagaaa gactccttta gggtaagaac ggataccacc
     6721 ataaccaatc agatgttcaa tttcttcata aatttccgtt tcattcaaat ccatttcacg
     6781 taatactttt ccagcaattc cattttgctc gaccactaag gctagtaaaa catgttctga
     6841 actaactgat tgatgtttca accgttttgc ttcttcttgt gcaatttcta atactgcttg
     6901 ggctctttca gtaaaaagtt cttccatcta atctcacttc ccatcttcat aacttaaacg
     6961 ttccagaaat gctcggagaa tccgagcacg tgtatgatct tcatattcat tgaaattcaa
     7021 tgtgtttttt gcaattgcac tcaacatcaa gtttccttct ttttttgtaa ttatttggtc
     7081 ttcataaagt ttttgaataa tagcaaaagc atttttctca ctgattgtat catcaaataa
     7141 ctgatttatc tgctcaagta attgtttttt atctgaaatc cttacttttg caattcgtat
     7201 ataaccacca ccaccacgtt tgctttctac tagataacca cgttggatgg taaaacgggt
     7261 attaatcaca tagttgattt gtgaaggaac acagttaaag aggtctgcca tttcaatcct
     7321 tcgaatctcg atcatttcac tattttccag aattttcttt atatatgatt caattaaatc
     7381 cgaggtattc tgatgtgcca tcttattcat tccctttgac taatattgac cttaattata
     7441 acgaatttga taatagaaag aaagaagaac gctttttgtt aaataaatcc tttagaatca
     7501 acaaaaaaaa atacggaata tactattccg tatcgacgcg cccaagagga gtcgaacccc
     7561 taaccttttg atccgtagtc aaacactcta tccagttgag ctatgggcgc atattcaatt
     7621 taaaaaaatt ataaatcatt tgaaaacaaa aagcaagata aaaaaaatcg ggaagacagg
     7681 attcgaacct gcgacccctt ggtcccaaac caagtgctct accaagctga gctacttccc
     7741 gtttaaaaaa taaaaaagac
//
LOCUS       DACGLE010000101         7597 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0534
            SAMEA3304449-rid9560453.denovo.107, whole genome shotgun sequence.
ACCESSION   DACGLE010000101 DACGLE010000000
VERSION     DACGLE010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304449
            Sequence Read Archive: ERR985064
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7597)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7597)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702225.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702225.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 20:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,045
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 27 of 67
            Pseudo Genes (incomplete)         :: 25 of 67
            Pseudo Genes (internal stop)      :: 19 of 67
            Pseudo Genes (multiple problems)  :: 4 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7597
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304449-rid9560453.denovo.107"
                     /strain="VREN0534"
                     /db_xref="taxon:1352"
     gene            complement(135..425)
                     /locus_tag="IXT20_002537"
     CDS             complement(135..425)
                     /locus_tag="IXT20_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4860951.1"
                     /translation="MGEKKIKGAGILLVMSKALFLLPCTFMFIVLCYFKRRKKWCWID
                     VLLFLPFFPLIYFLEEIWDDMACDSAFLFSHSLGLMISLTIFAIVLFFLNKK"
     gene            complement(543..803)
                     /locus_tag="IXT20_002538"
     CDS             complement(543..803)
                     /locus_tag="IXT20_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4860952.1"
                     /translation="MGVDHLIVLSKLLFWIPFIIMTSAFFYLFKRPNKYLLLSACPMI
                     YFIHKIRGFYYYDSGISLFCYEIGLLASAIFFIFVLEYFRKK"
     gene            complement(940..1353)
                     /locus_tag="IXT20_002539"
     CDS             complement(940..1353)
                     /locus_tag="IXT20_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HAQ4860953.1"
                     /translation="MANVPSIRDMFPDFNDVFSPAFNDFLGVSSYPKVDLVENEKEYK
                     LTADMPGCDKEDTTVEYSDNTLTISANHESHTEDKEDGNYVRKERHSVSYKRSFYLPN
                     VDEEKITGTFKNGVLKLVLPKTAYQPKETKKIELN"
     gene            complement(1809..2294)
                     /locus_tag="IXT20_002540"
     CDS             complement(1809..2294)
                     /locus_tag="IXT20_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4860954.1"
                     /translation="MHKGIYAGLLGTVILFSACGYKTKNSVKESSAAISTTEVGSTKS
                     TSTTSITNTSTGNVDSQHSQSSIQSFSISSTSEMQESTVPSLPLENSDKALLFLIAHR
                     SELQSDDIVISFYQKIDRDYLFTASSKQIRSQGGSGSVGFYRVSPEGSITMTDANGTP
                     F"
     gene            complement(2433..2648)
                     /locus_tag="IXT20_002541"
     CDS             complement(2433..2648)
                     /locus_tag="IXT20_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF378 domain-containing protein"
                     /protein_id="HAQ4860955.1"
                     /translation="MKTLDAIALTLLIVGGLNWLLVGLFELDLVAMIAGGSTTIFAKI
                     IYIVVGICAIYCLKFFPMITRKVDERY"
     gene            2872..3672
                     /locus_tag="IXT20_002542"
     CDS             2872..3672
                     /locus_tag="IXT20_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAQ4860956.1"
                     /translation="MSDWLDIAGKVVIVTGGSSGIGRSIVENLLKQNAQVANFDVTEC
                     RIQHENLLSLKVDVSSKTDIEEGIYKVMKHFKTIDGLVNNAGINIPSLLIDRNHPKSK
                     YELSEQVFDKMIAVNQKSVYLMSQAVGRILVQKGSGVIVNLSSESGLEGSEGQSCYAA
                     TKAAMNSFTRSWAKELGKRNVRVVGVAPGILEETGLRTQEYEEALSYTRGISVEQLRN
                     GYSRTSTIPLGRSGKLQEVADLVCYYLSERSSYITGVTTNISGGKTRG"
     gene            3691..5559
                     /locus_tag="IXT20_002543"
     CDS             3691..5559
                     /locus_tag="IXT20_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAQ4860957.1"
                     /translation="MTLVNRWYQLLKLLVTHKKVSIQEVQKEIKTSNQTIKKDIGELN
                     EEIAGIAKIIQKNKHFELEIYDFDFFDEIMQGKLKTASDFNSASKRIAYLLNRLIETE
                     DFLRMDDLSEEVGVSRGTVVKDLKTLKEMINDFDVKITGTPNKGLRIIGDELELRLLL
                     FYFVSPYFSETKTEQFLEEKQAIKHFQKKTNASKQEITLLTKVIGISLNRISSNYLLK
                     KPINRYSGCFEYIQEFNELINQLEEIYELTLSQYEKDFISFPLNIINTSITMIRKANS
                     DLRLYFDPMMMRIRRLDLIDLDESFLYQEMKQHLSLLINRVIFRYRLTDLFYGEIEKK
                     YPFSYQIASECIQALEAILQHKISKVEISYLTLYLELAFRYQKEHFSSKKVAIVHTTG
                     KGTALMIQRQIKRILGRDIQITHVSEEQYEKVDLNKYFAVFTTIPLKNVNEETPVIHL
                     TNLFDDSWLRAQWQKAKKKRLAGFHSTVFFYQIWKEKKTYQEYLNKLVAPLIRGYYVD
                     EEFEKRILKREYQQSTFVNKEFAFPHTTNQLSQHIVLSVGLITDQGLAETGGNGVIFL
                     FAIPEEMTAEVENELFELYDILFELIKMGIDEFIELAPTQESFLKLIKSKGERLHE"
     gene            5552..6043
                     /locus_tag="IXT20_002544"
     CDS             5552..6043
                     /locus_tag="IXT20_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR family transcriptional regulator"
                     /protein_id="HAQ4860958.1"
                     /translation="MNDMYLFIVFAASAYVLQAVISWKQLKHFNQTYSELRRKGKVAI
                     GKRPGKIRSGTIVMLAIDDHDQVLEVRKMQGVTSLAKFHSLPVYAGEDIHYIDRYHPS
                     VRAEDRLTQAALENAREVYLRIEAGNEKSEQPSLPFIAMLNNFRIWKSTIKEKRSDKR
                     WIS"
     gene            6031..6585
                     /gene="srlA"
                     /locus_tag="IXT20_002545"
     CDS             6031..6585
                     /gene="srlA"
                     /locus_tag="IXT20_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174078.1"
                     /note="catalyzes the phosphorylation of incoming sugar
                     substrates along with their translocation across the cell
                     membrane; the IIC domain forms the PTS system
                     translocation channel and contains the specific
                     substrate-binding site; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HAQ4860959.1"
                     /translation="MDIITKFAEGFMRLFQTGAETFISWMTGIVPVVLMLMVAMNTLI
                     AFLGEDRVTKVAKLSAKNPLTRYLVLPFLSAFMLGNPMSFTMARFLPEYYKPSYYAAQ
                     AQFCHTSNGVFPHINPGELFVWMGIAQGIETLGLNSMELAIRYLLVGLLMNFIGGWIT
                     DFTTGFVCRQQGIVLSKKVELSVD"
     gene            6603..>7597
                     /locus_tag="IXT20_002546"
     CDS             6603..>7597
                     /locus_tag="IXT20_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HAQ4860960.1"
                     /translation="MYGDIKIVRGNGGYGGPLIIRPTKDKHKFIYVTGGGERPMIVDK
                     IEELTGMESINGFKTSIPDSEIALAIVDCGGTLRCGIYPKKGIPTINIVSTGKSGPLA
                     QYITEDIYVSAVGLEQIQPLMETDDNKETVNTASPDKKYKYDTSKKITEQKAEQQNWI
                     AKIGMGAGKVVATFNQSAREAVDTMIRTVIPFMAFVSLLIGVIQGSGIGNVFANIMAP
                     LAGNAVGLTIIGFICSLPFLSPLLGPGAVISQVIGTLIGVEIGKGNIPPQLALPALFA
                     INTQNACDFIPVGLGLEEAKAETVEVGVPSVLYSRFLNGIPRVIIAWVASFGLYS"
BASE COUNT         2707 a         1213 c         1443 g         2234 t
ORIGIN      
        1 gttactatga ataacataat aagaaagtaa agtggattga tttgagatct ttttatactt
       61 caaaaaagat aacacccaac tttcaaaaat atgttttgga agttgggaca aacgagttaa
      121 tacattttaa tacattattt tttattcaaa aaaaatagca caatggcaaa gatcgtaaga
      181 gagatcataa gccccaatga atgactaaaa agaaaagcag aatcacatgc catatcatcc
      241 catatttctt caagaaaata aataagcggg aaaaagggaa ggaatagtag aacatcgatc
      301 cagcaccatt tttttcttct tttgaaataa cataaaacaa taaacatgaa cgtgcaggga
      361 agtaaaaaca aagctttact cataactaac aaaataccag ctccttttat ttttttctcc
      421 cccacattca aagagtaaca atggtatgta cttaataata gaaaaatgga tcaacttgta
      481 tgaataacag ctgaaaagat ctgcaccaaa taaaaaactt ccagccaaaa ctgactggaa
      541 gtttattttt ttctgaaata ttctaacaca aatatgaaaa agatggccga agctaataag
      601 ccgatttcgt agcaaaacaa tgagatacca gagtcgtaat aataaaatcc tctaatctta
      661 tggataaaat agatcatcgg acaagcagat aagagtaagt atttgttagg ccttttgaat
      721 aagtaaaaga aagcactggt cattatgata aatggtatcc aaaacaaaag tttacttaag
      781 acaatcaaat gatcaactcc catatttttt accaagagag atcataacat ctttatcgta
      841 aaaacaaaat atttttttgc tcattttgaa aatttaaaca tactcgttct tatccaaata
      901 tataaaaatc gcccctcatt catcgagagg cgataagttc taatttaact caattttttt
      961 tgtttctttt ggctgataag ctgttttagg taataccaat ttcagcacac cgtttttaaa
     1021 ggttccagtg attttttctt catcaacatt aggaagatag aatgaacgtt tgtaagagac
     1081 cgaatgtctt tctttccgta catagttgcc atcttcttta tcttctgtat gggactcatg
     1141 attggcggag atcgtcaaag tattatctga atattcaact gtcgtgtctt ctttgtcaca
     1201 gccaggcata tcagcagtta gtttgtattc tttttcattt tcgactaaat ctacttttgg
     1261 ataacttgaa acacctaaaa aatcattgaa tgctggacta aacacgtcat tgaaatcagg
     1321 gaacatgtcg cgaatgctag gtacatttgc catttttgat catcctttca ataagtattt
     1381 tttatttatc cctatttacg taataactct attttgaaaa aaatgcaagt ataatggtta
     1441 gctttgtgaa tatttcatct aatacgatac aaaggctatc tttctaattt gaaaaaggga
     1501 tcattaaaat aataagaaga aaatatgtca aaaggtaaaa aagtaaattc ttctatccaa
     1561 atatagaaaa attgaatcta gaaaaccgcc cgacaataaa tttcattaac tcagttgacc
     1621 gaaaatgaag tagataccaa aaatttatca agagagcaag tagagaagtg agtatattca
     1681 taagagaaat agctgtaacg aattattagg ttaatgcaaa tgcttattta tactccgtat
     1741 aactttctca gaaaaaatga taccaataat gacttgctag tactgtaaat ccgtataata
     1801 tcatctaatc aaaatggtgt tccatttgca tctgtcatcg taatcgatcc ttcgggtgac
     1861 actcgataaa aaccaacgct tccagatcct ccttgggaac ggatttgttt gctggaagct
     1921 gtgaacaaat aatctcgatc aattttttga taaaagctaa taacaatatc gtcactttgt
     1981 aattcggaac ggtgggcaat aagaaataga agtgctttgt cagagttttc taatggaaga
     2041 gaaggtacag tgctttcttg catttcagat gttgaagaaa tgctaaacga ctgaatactt
     2101 gattggctat gttgcgaatc tacattacca gtagaagtat tagtaatgga agtagtagaa
     2161 gtacttttag ttgaaccaac ctcagttgtt gaaatggctg ctgaagattc tttaacagaa
     2221 ttctttgttt tatatccaca ggcagaaaaa agtattacag ttcccaacaa gcctgcgtaa
     2281 atccctttat gcatgatagc tcctcctaaa atagattatc tttattcagt atatcactta
     2341 aacaaagaaa agcagatggt ttaaaatgaa aaaaccttcc caataaaggg aaggttcatg
     2401 aagataataa cttttttaat aattatagct ttctaatatc tttcatcaac ttttctagta
     2461 atcattggga aaaatttcaa gcagtagatc gcacaaatac ctacgacgat ataaatgatc
     2521 ttcgcaaaaa tagttgttga accaccagca atcatagcta ctaaatcaag ttcgaacaag
     2581 cctaccaaca gccagttcaa ccctcccaca atcaataaag ttaatgcaat agcatctaaa
     2641 gttttcatta gaattctcct ttcttaaatg ataaaacaat tatatatcaa aaagagaaac
     2701 attgcttata taacgctcag gaattttagc aatctatgca aaaagatgaa aatttttttt
     2761 gctcttccat tagtacacat tttttagctg aagctgtaaa tttcatgtct taattttttt
     2821 tagtgagctt ttatactgga tttgtaagaa aaaagaagga gaggatcaca tatgtcggat
     2881 tggttggata ttgcaggaaa agtagtaatt gtaactggag gttcctcagg aattggaaga
     2941 agtatcgtcg aaaatttgtt gaagcagaat gcacaagtag cgaattttga tgtaactgaa
     3001 tgccgtatac agcatgaaaa cctactatct ttaaaagtcg atgtgtcttc taaaacggac
     3061 atagaagaag ggatatataa agtaatgaaa catttcaaga cgatcgatgg tctagtcaat
     3121 aatgcaggaa taaatattcc ttctcttcta atagatagaa atcatccaaa aagcaagtat
     3181 gaattatcag aacaagtttt tgataagatg atcgctgtca atcaaaaaag tgtatatttg
     3241 atgagccaag cagttggaag aatcttggtg caaaagggat caggagtgat cgtgaatctt
     3301 tcttctgaat caggattgga aggatcagaa ggacagagct gttatgctgc tacaaaagca
     3361 gcgatgaaca gttttactcg ttcttgggca aaagagttag gaaagaggaa tgttcgtgtg
     3421 gtaggtgttg caccagggat actggaagaa accggtttga gaacacaaga atatgaagaa
     3481 gctctttctt atacgagagg tataagcgtc gaacagctga gaaacggtta cagtcgaaca
     3541 agtacgatcc ctctaggaag gagtggtaag ctgcaagaag tggcggatct agtctgctat
     3601 tatctttcag aaagatcaag ttatatcact ggagtcacga ctaatatttc aggaggtaaa
     3661 acaagaggat aaaggatagg ggggacgcac gtgacgctag taaacagatg gtaccaatta
     3721 ttgaaattac tagtcactca caagaaagtt tcaatccagg aagtgcaaaa agaaatcaaa
     3781 accagtaatc aaaccataaa aaaagatatt ggggagttga atgaagaaat tgccggtatt
     3841 gcaaaaatca tccaaaaaaa caaacatttt gaacttgaaa tttatgattt tgattttttt
     3901 gatgagatca tgcaaggaaa attgaaaaca gccagtgatt tcaattcggc atccaaaaga
     3961 atcgcttatt tactaaatag gctgatcgaa acagaggatt tcttacgtat ggatgatttg
     4021 tctgaagaag tcggtgtcag cagaggaaca gtggtcaagg atctaaagac attaaaagag
     4081 atgatcaatg attttgatgt aaagattaca ggcacaccaa ataaaggact aagaataata
     4141 ggagatgagc tagaattacg actactttta ttttattttg tttctcccta cttttcagag
     4201 acgaaaacag aacagtttct agaagaaaaa caagcaatca aacattttca gaagaagacg
     4261 aatgctagta aacaagaaat caccctttta acgaaagtta tcggtatttc tttgaataga
     4321 atctctagta actatctgtt gaagaagccc atcaatcgtt acagtgggtg ttttgaatat
     4381 atccaagaat tcaatgaact gatcaatcag ttggaagaaa tctatgaact aactttgagc
     4441 caatatgaaa aagattttat cagttttcct ttgaacatca tcaacacaag tataacgatg
     4501 ataaggaaag ctaactctga tttgcgctta tatttcgacc ccatgatgat gcgtattcga
     4561 cgattagatc tcattgatct ggatgagtcc ttcttatatc aagagatgaa acagcacctc
     4621 tctttattga taaatcgggt aatttttcgt tatcgattga ctgatttatt ttatggtgag
     4681 atagaaaaaa agtatccttt ttcttaccaa atcgctagcg aatgcataca agcattagaa
     4741 gctatcttgc aacataagat ctctaaagta gagatcagct acctaacgtt gtatttggag
     4801 ttagcatttc gatatcaaaa agagcatttt tcctctaaaa aagtagccat tgttcatacg
     4861 acaggaaaag gaacagcatt aatgatccaa agacaaatca aaaggatatt ggggagggat
     4921 atacaaataa cacatgtttc agaagaacaa tatgaaaaag tagatctaaa caaatatttc
     4981 gctgttttta caacgattcc gttaaaaaat gtaaatgaag agacaccggt gatccatttg
     5041 actaatcttt ttgatgactc ttggttaagg gcacaatggc aaaaagcaaa aaaaaagcga
     5101 ttagccgggt ttcattctac tgtttttttc tatcaaatat ggaaagaaaa aaagacgtat
     5161 caagaatatt taaacaaatt agtcgctcct ttgatcagag gctattatgt ggatgaagaa
     5221 tttgaaaaaa ggatcttgaa aagagaatat cagcaaagca catttgttaa caaagaattt
     5281 gcttttcctc acacaaccaa ccagctatct caacacattg ttttatcagt agggctaata
     5341 actgatcaag gtttagcaga aacaggagga aatggagtta tttttttatt tgccatacct
     5401 gaagaaatga cggctgaagt tgagaatgaa ttatttgagc tatacgatat cctatttgaa
     5461 ctcataaaaa tgggaataga tgagtttatt gaattagcac cgacacaaga atcattttta
     5521 aaactaatca aaagtaaagg agagagactt catgaatgat atgtatctat tcatagtttt
     5581 cgcagcttca gcttatgtgc tgcaagcagt tatcagttgg aagcaactaa agcatttcaa
     5641 tcaaacatat agcgaactgc gaagaaaagg aaaagtagcg attggtaaac gacctgggaa
     5701 aatccgttca ggcacgattg ttatgctagc tatagacgat catgatcaag ttttagaagt
     5761 cagaaaaatg caaggggtta catctttggc aaaatttcat tcattacctg tctatgcggg
     5821 tgaagatatc cattacatcg atcgttacca tccctcagtt cgagcggaag atcgtttgac
     5881 acaagcagct ttagaaaatg ccagagaagt atacttacga atagaggcag gaaatgaaaa
     5941 aagtgaacaa ccttcattgc cttttattgc tatgctcaat aattttcgga tatggaaaag
     6001 tacgatcaaa gaaaaaagga gtgacaaacg atggatatca taacaaaatt tgctgaggga
     6061 tttatgaggc tgttccaaac aggtgcagaa acattcataa gctggatgac aggaatcgta
     6121 ccagttgtct tgatgctgat ggttgcaatg aatacgctga ttgctttttt aggtgaagac
     6181 cgagtaacga aagtagccaa attatcagcg aaaaatccgc ttactcgtta ccttgtgtta
     6241 ccgttcttat ctgcttttat gttgggaaat cctatgtcat ttacaatggc tcgtttcctt
     6301 ccagaatact acaaaccaag ctattatgct gctcaagctc aattttgtca tacgagcaat
     6361 ggtgtattcc cccatattaa tcctggagaa ttgtttgtat ggatggggat tgctcaaggg
     6421 atcgaaacct tagggctaaa ttcgatggag ctggctatcc gatacttgct tgtgggactg
     6481 ctgatgaact ttattggtgg ttggataact gattttacta ctggatttgt atgcagacag
     6541 caaggaatcg ttcttagcaa aaaagtagag ctttcagttg attagagaaa ggagagtttt
     6601 aaatgtatgg tgatattaag atcgtaagag gaaacggcgg gtatggaggt cctttgatta
     6661 ttaggccgac aaaagataaa cacaagttca tctatgtcac aggtggaggt gagaggccaa
     6721 tgatcgtaga taaaatagaa gaactgacag gtatggagtc tatcaatggg ttcaaaacat
     6781 caatacctga tagtgaaatc gcgcttgcaa tcgttgactg tggagggact cttagatgtg
     6841 gaatttatcc taaaaaaggg ataccaacca tcaatatcgt ttccacagga aaaagtggcc
     6901 cattagcaca atatatcact gaagatattt atgtttcagc agtagggtta gaacaaatac
     6961 agccactaat ggaaactgat gataacaaag aaacagtaaa tactgcatct ccagataaaa
     7021 agtacaaata tgatacttct aaaaagataa ccgagcaaaa agcagagcag caaaactgga
     7081 tagcaaaaat tggaatgggc gcgggaaaag tagttgctac attcaatcaa tcagcaagag
     7141 aagcagtaga tacgatgatc cggacagtga tcccttttat ggctttcgtt tcccttttga
     7201 ttggtgtgat acaaggttct gggatcggta atgtatttgc taacattatg gcacctttag
     7261 caggaaatgc agttggatta acaatcattg gctttatttg ttcactgcca tttttatctc
     7321 ctttgcttgg tccaggggca gttattagcc aagtgattgg aacattgatc ggtgtagaga
     7381 ttgggaaagg aaatattcct ccccaattag cattaccggc actatttgct atcaatactc
     7441 aaaatgcatg tgattttatt ccagtcggtc taggtttgga agaagcaaaa gctgagacag
     7501 tagaagtagg tgtaccatca gttctttatt caagatttct taacggcatc ccacgagtta
     7561 tcattgcttg ggtagcaagt tttggattat atagctg
//
LOCUS       DACGLE010000102         7590 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0534
            SAMEA3304449-rid9560453.denovo.108, whole genome shotgun sequence.
ACCESSION   DACGLE010000102 DACGLE010000000
VERSION     DACGLE010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304449
            Sequence Read Archive: ERR985064
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7590)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7590)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702225.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702225.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 20:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,045
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 27 of 67
            Pseudo Genes (incomplete)         :: 25 of 67
            Pseudo Genes (internal stop)      :: 19 of 67
            Pseudo Genes (multiple problems)  :: 4 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7590
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304449-rid9560453.denovo.108"
                     /strain="VREN0534"
                     /db_xref="taxon:1352"
     gene            complement(83..1114)
                     /locus_tag="IXT20_002547"
     CDS             complement(83..1114)
                     /locus_tag="IXT20_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ4860961.1"
                     /translation="MFLEIHDLNLFFDEKQILAIDEIQIEKGELVTLLGPSGCGKSTL
                     LRCITGLEQPKSGEIVLDNENIADKPTKDRNIGFVFQQYALFPTMTVFENVAFGLKVK
                     KLSPEVIQEKVFEMLSLVDMTEQADKNVQFLSGGQRQRVALARSLVTEPKVLLLDEPL
                     SALDARIRKQLQRDLRAIQQSLGMTMIFVTHDQEEAMRISDRIFVMEAGRVAQVSTSK
                     ELYQNPQSRFVAEFIGNYNRFEWYELNQYTHEFSAKGNRCIYYLRPELIQFEPVPQGV
                     KIPVLWKESFILGNIQRYVFQTLEGKEILVDRLNDREEKISDALYIQLKDILAIPVEI
                     DTQHFAQQS"
     gene            complement(1121..1951)
                     /locus_tag="IXT20_002548"
     CDS             complement(1121..1951)
                     /locus_tag="IXT20_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ4860962.1"
                     /translation="MKKLIKPILFCFVLYYLLPIIGTILYASSTKWSKSLLPSDFTLQ
                     WFQQLLTDREFIAAVGRSLLLAGVVLVTILLLMIPTIIWIHLYFPRLNRWLEKLLLLP
                     YALPGVILVTALLRTYAKTGIPMFVVLVGALFITALPIVYLSLNNQMRLINLKELVDA
                     AETLGAPMSTIIIQVLFPNIRIGVTLVSLMIFSSVFGEYMLTNLLIGGRFQTVRIYML
                     RRMNENGHLASAVMVLYLLFMVGIALTTFMLTYRQKQQLSLKQTREEKQSKKKPLKEL
                     "
     gene            complement(1948..2754)
                     /locus_tag="IXT20_002549"
     CDS             complement(1948..2754)
                     /locus_tag="IXT20_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAQ4860963.1"
                     /translation="MKKRLVLVPASFIFCAFLLLPLLFMVLCSLHSDLTNQWTLANYS
                     DILSNKYYTQAFVNSVLIAVSSSVIGLIGTFILCLCLMNLSTKLQEKITFVSNLAANF
                     AGIPLAFSFIILLGNVGVLKMILPFLADFNLYSWWGLTITYTYFQIPLGVLFLYPSMK
                     EVKKEWLESAQLLGASSAYAWFKVALPFLRPSIASTFVILFANGMGTYETAYALTSSN
                     VNLLTIRIAALVSGDVFAKPNLGSALAVALGVLLVTAMLIIQKKGKVVQT"
     gene            complement(2760..3584)
                     /locus_tag="IXT20_002550"
     CDS             complement(2760..3584)
                     /locus_tag="IXT20_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphatase"
                     /protein_id="HAQ4860964.1"
                     /translation="MQSRLDKLIVVILDGCRYDTAVAQLGFMNHLVEHQQASLLKVKS
                     ELPSNSRPLYEVLMTGVPAYENGIYSNYHAQRSKEWSLFELVKEAGGKTGAAAYHWYS
                     ELYNQAPYNRLVDRIQHQEERLIQHGIFYSEDTYPDSHLFADAHYLVNQYQPDFLVVH
                     SMNIDDVGHKFTADSHEYAAAVNQADRLLAIYLPEWLQKGYQVVVTADHGMDAHGLHG
                     GSLAAHREVPFYLISNKRQKLKEQTVPQLLAAPLFCWLLGIAPSEKMQDIQELMRG"
     gene            complement(3574..4674)
                     /locus_tag="IXT20_002551"
     CDS             complement(3574..4674)
                     /locus_tag="IXT20_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAQ4860965.1"
                     /translation="MKMNEKIRNMTIGLFCAATVSILLTGCGSGEATKQSAAADDSLD
                     TIIEKAKEEGEIASLGMPDTWANWVGTWNDLEKEYGLSHVDTDMSSAEELAKFESEGK
                     NGTADIGDVGISFGPLAESKELTLPYKTNYWEEIPDWAKDDDGDWLLSYTGTISFITD
                     KENVKQAPTSWKELQEGDYQVSVGDVTIANQAQFAVLAAAIANGGDEKDIQPGLNYYK
                     KMAEKGRLSTVDASVANLEKGEIDVAVVWDFNGLNYRDQIDSERFEVTVPTDGSIISG
                     YTTLINKNAPHPNAAKLAREYILSDEGQINLAKGYARPIRKVELPKEVAEKLLPEDAY
                     EAAVPVEDNEAWNKTSVELPELWQAEVLTYAK"
     gene            4852..5637
                     /locus_tag="IXT20_002552"
     CDS             4852..5637
                     /locus_tag="IXT20_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol phosphate phosphatase"
                     /protein_id="HAQ4860966.1"
                     /translation="MQYYDQHLHTYFSFDSEEKFENYLAYQPEFFVSTDHFDLNNPST
                     GKDDIPDYPAYINKLDSLKETYSTKFLRGIEIGVVPGQEERIVDYLAQHPYDLKLVSI
                     HQNGRFDYMDDVVLTKDPIQTTKEYFDQMLQVLTDFPEGDILTHFDYGLRRFTFTAEE
                     FAEHFEAQLLTIFKKVVERELAMELNAKSFLKYGNEDLYRYAVPLYQSVGGKLFTLGS
                     DAHVAEDYQLGFKKMSQLLADCQVEKLLVIQGKERRLTPLPIL"
     gene            5670..6053
                     /locus_tag="IXT20_002553"
     CDS             5670..6053
                     /locus_tag="IXT20_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HAQ4860967.1"
                     /translation="MSNLRDYNQEAPIHCLIARHWDALKIEAVCRSLLAAVPKQQLEN
                     FLVADSLQREKVQAYFAAFKDQPLEYLHAQFHLFYQVAAPDDYNDLRGQLQLTFQADE
                     TAYTVLLGMARLGDQAKVEWRIFDI"
     gene            6129..6260
                     /locus_tag="IXT20_002554"
     CDS             6129..6260
                     /locus_tag="IXT20_002554"
                     /inference="COORDINATES: protein motif:HMM:NF012387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAQ4860968.1"
                     /translation="MLVTDAGEIRLRLFPDQAPLAVANWRSLAEQGVYDNTPFGSCH"
     gene            6229..6465
                     /locus_tag="IXT20_002555"
     CDS             6229..6465
                     /locus_tag="IXT20_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAQ4860969.1"
                     /translation="MTTHLSARVIKEFVIQGGALDGSGDEAVSSYEGFFADEVHRGLY
                     HFNGALALGDHGPHTNGNQFFIVQNTKAQADLLM"
     gene            complement(6543..7514)
                     /locus_tag="IXT20_002556"
     CDS             complement(6543..7514)
                     /locus_tag="IXT20_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HAQ4860970.1"
                     /translation="MEGTVLRIEQGSLHDGAGLRTVVYLKGCPLRCAWCSIPESQSKQ
                     IEKGFGQTMTAEEVMDEIEKDAVFYFHSDGGVTISGGEALVQADFAKEILQKSKYIGI
                     NTVLETSFCGAYNEIQKVAPYVDTLFVDVKMFTSKLHKQWTGLDNQQILKNIRRFLIE
                     YPNCEVRIRVPVVPGINMNLTELLTIACFVADLDRFVPLELLPYHCYGMHGYQALGLE
                     YPLADTPAPSPKEMFALADQLARTVPHLLVTALTKTFIYLLNPSFILWINERHSLAAA
                     SSRIELLLKSVSVFLRCFFEVNLSNEHWLNRYIFTVCRLSCDRCYDF"
BASE COUNT         2175 a         1812 c         1508 g         2095 t
ORIGIN      
        1 aaatcaattc taccttagat gttcttatat cactctatca gtcctatttg acaaagagcc
       61 agaaatgttt gccgactata cactagcttt gttgtgcaaa atgctgagta tcaatttcca
      121 cagggattgc tagaatatct ttcagctgaa tgtataaggc atcgctaatt ttctcttccc
      181 gatcgttcaa ccgatccact aaaatttctt tgccctccag cgtttgaaaa acataccgtt
      241 gaatgttgcc taaaataaat gattccttcc atagaaccgg aatcttaacc ccctgaggaa
      301 ccggttcaaa ttgaatcagc tccggtctta aataataaat gcagcgattg cccttagcgg
      361 aaaactcgtg ggtatactga ttcagctcat accattcgaa acgattatag ttgccgataa
      421 attccgcaac aaatcggctc tgaggattct gatacaattc cttcgaggtg gaaacctgag
      481 ccacccgccc agcttccatc acaaaaatcc gatcactgat tcgcatcgcc tcttcttggt
      541 catgagtcac aaaaatcatc gtcatcccta gcgattgctg gatagcgcgc aaatcccgct
      601 gcagctgttt tctaattcgg gcatccaaag cacttaatgg ctcatccagc agcaggactt
      661 tcggttcagt gaccaatgag cgagccagtg ccacccgttg tcgttgaccg ccagaaagaa
      721 actggacatt tttatctgcc tgctccgtca tatccaccaa tgatagcatc tcaaaaacct
      781 tttcctgaat cacctcagga ctcagcttct ttaccttcaa accaaaggct acattttcaa
      841 aaacagtcat agttggaaac aatgcatact gttgaaaaac aaagccgata ttgcgatcct
      901 tcgtaggctt atcggcaata ttctcattgt ccagcacaat ctcaccgctc tttggttgtt
      961 ccaatcccgt gatacagcgc agcaacgtag actttccgca cccgctggga cctagtaaag
     1021 taaccagctc acccttttca atctgaattt catcaatagc taaaatctgt ttttcatcaa
     1081 aaaacagatt gagatcatgt atttctaaaa acattttccg tcacaactcc tttaagggct
     1141 tctttttcga ctgcttctct tccctcgtct gcttaaggga cagctgttgc ttttgtcgat
     1201 aagtcagcat aaaggtagtt aaagcaattc ccaccataaa cagcaaatac aaaaccatca
     1261 cagcgctagc caaatggccg ttttcgttca tccgccgcag catatagatg cgtaccgtct
     1321 gaaatcgacc gccaatcaac aggttcgtca gcatgtactc cccaaaaacc gaagaaaaaa
     1381 tcatcagaga aactagggtc acaccgatac ggatgtttgg aaacagcacc tggataataa
     1441 tcgtagacat cggggcgccc aaggtttctg ctgcatccac taattccttc agattaatta
     1501 agcgcatctg gttgttaaga ctaagataga caatcggcaa cgccgtaata aacaatgcac
     1561 ctaccaacac tacaaacatt gggataccag ttttagcata cgttcgaagc aaagccgtta
     1621 ccaaaatcac tcctggtaaa gcatacggca gcaggagcag cttttccagc caacgattca
     1681 aacgagggaa atacaaatga atccaaataa tggtgggaat catcaacaac agaatcgtca
     1741 ccaagaccac acctgctaat aaaagtgagc gacccaccgc agcaataaat tccctgtccg
     1801 tcagcagctg ttggaaccat tgcaaggtaa aatcactagg taaaagcgat tttgaccatt
     1861 tagtggagct ggcatacaaa atcgttccaa taatcggcag caagtaatac aggacaaagc
     1921 agaacaaaat cggttttatt aactttttca cgtctgcacc accttgcctt tcttttgaat
     1981 aatcaacatt gccgttacca gcaggacacc taaagcgact gccaacgcac tgcctaaatt
     2041 aggcttggcg aacacatctc ccgacactaa agccgcaatt cgaatcgtta aaagatttac
     2101 attactgctg gtcagggcat acgctgtttc ataggtcccc atgccattag caaataagat
     2161 gacaaaggtg ctggcaatcg atggtcgcaa aaaaggcaaa gccaccttga accaagcata
     2221 cgctgatgaa gcgcccagca attgtgcgga ctccagccat tcctttttca cttccttcat
     2281 gctgggatac aaaaacaaga cccctaacgg aatttggaaa taggtatacg tgatggtcaa
     2341 tccccaccaa gaatacaaat tgaagtccgc tagaaatggc aaaatcattt tcagcacacc
     2401 tacattcccc aacaaaataa taaaagagaa tgccagcgga atcccagcaa aattagctgc
     2461 caaattggaa acaaaggtga ttttctcctg caatttagtc gatagattca ttaagcataa
     2521 acataaaata aacgtcccga ttaacccaat cacactggag ctgacagcaa tcagtacgct
     2581 gttaacaaag gcctgtgtgt aatatttgtt gcttaaaata tcactgtaat tggccaatgt
     2641 ccactggtta gttaaatcac tgtgcaagct gcacagaacc ataaaaagca gcggcagcag
     2701 taaaaatgca cagaagataa aggaggcagg aaccagaacc aagcgttttt tcatctcttt
     2761 catcccctca tcaattcttg aatgtcctgc attttctctg acggtgcaat tcctaataac
     2821 cagcaaaaca atggagccgc cagcagctgt ggtacagttt gttccttcag cttttggcgt
     2881 ttatttgaaa tcaaataaaa aggaacttca cgatgtgcag ccaagctgcc gccatgcagt
     2941 ccgtgcgcat ccatcccgtg atccgcagtc accacgactt gataaccttt ttgcagccat
     3001 tcaggaaggt aaatagccaa taatctgtcc gcctgattaa ccgctgctgc atattcatga
     3061 gaatcggcgg taaatttgtg accgacatca tcaatattca tcgaatgtac gaccaaaaag
     3121 tctggctgat actgattcac taaataatgg gcatccgcaa ataaatgcga atcagggtaa
     3181 gtgtcctcac tataaaagat gccatgctga atcagtcgct cctcctgatg ctggatgcga
     3241 tcaaccaaac gattataggg ggcttgattg tacaactcgc tgtaccagtg gtaagcggcg
     3301 gctcctgttt ttccgccagc ttccttaact aattcaaaca gcgaccactc tttggatcgc
     3361 tgagcatggt aattactata gatgccattt tcataggctg gaacgccggt catcagtact
     3421 tcgtataacg gacgtgaatt gctgggcagc tcacttttta ccttcaataa gcttgcttgc
     3481 tggtgctcaa ctaaatgatt catgaaccca agctgggcga cggctgtgtc gtagcgacag
     3541 ccgtctagta taactacaat cagtttgtca agtctacttt gcataggtca gtacctccgc
     3601 ctgccaaagc tctggaagct ctacagaggt cttgttccaa gcttcattgt cttcaactgg
     3661 caccgccgct tcataagcat cctccggcag taatttttca gctacttctt taggcagctc
     3721 taccttgcga ataggtcgcg catagccctt tgccaaattg atttgccctt catcagataa
     3781 aatatattcc cgtgctagct ttgccgcatt gggatgaggg gcatttttat tgattaaggt
     3841 tgtatacccg ctaatgatgg agccgtctgt tggcacagtt acctcaaaac gctcggagtc
     3901 aatttggtca cgataattta gtccattgaa gtcccaaaca actgccacat cgatttcacc
     3961 tttttcaaga tttgctacgc tggcatcaac ggtggacaaa cggccttttt ctgccatttt
     4021 tttgtagtaa ttcagacctg gctgaatatc cttttcgtca cctccgttgg caatcgccgc
     4081 agccaataca gcaaattgtg cttggttggc aatcgtcaca tcccccacgc taacttgata
     4141 gtcaccctct tgcagctcct tccagctagt cggtgcttgc ttcacattct ctttatcggt
     4201 gataaaggaa atggttcccg tatagcttaa caaccaatcc ccatcatcat cctttgccca
     4261 atccggaatt tcctcccaat agttggtctt ataaggtaag gttaactctt tgctttcagc
     4321 caacggaccg aagctgatac cgacatcacc aatatcggcg gttccatttt ttccttctga
     4381 ttcaaactta gccaattctt ctgcactgga catatccgta tccacgtgac ttaagccgta
     4441 ttctttttca aggtcgttcc aagtgcctac ccaatttgcc caagtatccg gcattcccaa
     4501 gctggcaatc tctccttctt ccttggcttt ttctataatc gtatctaaag agtcgtctgc
     4561 cgcagccgat tgcttcgttg cttcacctga gccgcagcct gtcagcagga tacttactgt
     4621 tgctgcacaa aataaaccga tagtcatgtt acgaattttt tcattcattt tcattcattc
     4681 ttctcccctt tcgataattc cattctaaca aagctttgta atgagagaat caatttcttg
     4741 aaaaggtttc atgaacaatg taaaggttgt atatggtttg taaatttcga cgaattcccg
     4801 ttaaccttct aaagcttttg cggtatgata gtagaaaaaa ggaggatccc tatgcagtat
     4861 tatgatcagc atctccacac gtatttttcc tttgattcag aggaaaaatt tgaaaactat
     4921 ttagcctatc agccggagtt tttcgtttct acggatcact ttgatttaaa caatccatct
     4981 accggaaaag atgacattcc ggattatcca gcctatataa acaaactgga ctctttaaaa
     5041 gaaacctatt ccaccaaatt tctgcgaggt atcgagattg gcgttgttcc aggacaagaa
     5101 gagcggatcg tggattacct cgctcagcat ccttatgatt taaagctggt aagtattcat
     5161 caaaatggcc gctttgatta tatggacgat gtggttttaa ccaaagatcc cattcaaact
     5221 accaaggaat attttgatca gatgttgcag gtcttgacgg attttccaga aggagatatt
     5281 ttgactcact ttgattatgg attacgccgc ttcaccttca cagcggaaga attcgcggag
     5341 cattttgagg ctcagctgct aacgattttc aaaaaggttg ttgaacgaga attggcgatg
     5401 gagttaaatg ccaagagctt cttgaaatac ggcaacgaag acctttaccg ttatgccgtt
     5461 cctttgtatc aaagtgtcgg cggaaagctc tttaccttag gctcggatgc tcatgttgcc
     5521 gaagattatc agctgggctt taaaaaaatg agtcagctgt tggcagactg tcaggtagaa
     5581 aagttgctag tcattcaagg aaaagaacgc cgcctgacgc cgctgccgat tttgtgattc
     5641 ctatataata gaagaaagtg gtgaaaaaaa tgagcaacct gcgggactac aatcaagagg
     5701 cacccatcca ttgtttaatt gcgcgacatt gggatgcgtt gaaaattgag gcggtttgcc
     5761 gatccttgtt agcagccgtt ccaaaacaac aactggaaaa ttttttggtg gctgacagct
     5821 tgcagcggga aaaagttcag gcttattttg cagcctttaa agatcagcct ttggaatatt
     5881 tgcatgcgca gtttcatctt ttttatcaag tagccgcccc agatgactac aacgatttac
     5941 gaggacagct gcaattgacc tttcaagcgg atgaaaccgc ctataccgtt ttattgggaa
     6001 tggctcgcct tggtgatcag gcgaaggtcg agtggcggat tttcgatatt tgatggcagg
     6061 atcaaggcgt ttcagttgca aaccttcctc tctttcaatt agaaaaaccg cagcaggaag
     6121 aagtttgtgt gctggtcacc gatgctggtg aaattcgtct ccgcttattt cctgaccaag
     6181 ctcccctggc tgtagccaac tggcgctccc tagctgagca aggcgtttat gacaacacac
     6241 ctttcggctc gtgtcattaa ggaatttgtt attcaaggtg gcgctttgga tggttctggt
     6301 gatgaagctg tttccagcta tgagggcttt ttcgctgatg aagtgcatcg cggactttat
     6361 cattttaatg gcgccttggc cttaggggac cacggccccc acaccaacgg caaccagttt
     6421 tttattgtgc aaaacacaaa ggcgcaggct gacctgctaa tgtaaagcaa gcatacttgg
     6481 agcttggagg actaccggag ctggatggtc gttatactgt ttttggataa gtataccaag
     6541 ggttagaaat cgtagcagcg atcgcagctc agccgacaaa cagtgaagat gtaccgattg
     6601 agccagtgtt cattcgacaa attaacttcg aaaaaacatc gtaaaaagac actcacagac
     6661 ttcagcaaaa gctctattct tgagctggct gcagctagtg agtgtctttc atttatccat
     6721 aagataaagc ttggattcaa taaataaata aatgttttgg ttaaagctgt gaccagcaaa
     6781 tgaggaaccg tgcgagccag ttgatctgcc aatgcaaaca tttctttagg tgaaggcgct
     6841 ggcgtgtctg ccaacggata ctccagtccc aatgcttggt aaccgtgcat accatagcag
     6901 tggtacggta gtaattctaa cggtacgaac cgatcaagat ctgccacaaa acaagcaatc
     6961 gtcagcagct cggtcaggtt catattgata cctggaacca caggaactcg aattcgcact
     7021 tcgcagttag gatactcaat taaaaagcga cgaatattct tcaaaatttg ttggttatct
     7081 aagccggtcc attgtttgtg gagcttgctg gtaaacattt ttacatccac aaacaatgta
     7141 tccacatagg gagctacctt ttggatttca ttataagcac cacaaaagct ggtttccagt
     7201 acggtattga ttccaatgta tttcgatttt tgcaaaattt ctttagcaaa atccgcctga
     7261 accagtgcct cgcctccgct aatggtcact ccgccatccg agtgaaagta aaaaacagca
     7321 tccttctcaa tctcatccat tacctcttca gcggtcatgg tctggccaaa tcctttttca
     7381 atctgcttgc tttgtgattc gggaatcgaa caccaggcac agcgtaatgg gcagcccttt
     7441 aaataaacaa cggtccgtaa accagcacca tcatgtagag aaccctgttc tattcttaat
     7501 actgttcctt ccatatagat ttttgaatta cacactagtt taaactaaaa cacacaagga
     7561 aaaacttacc atttaatcat aaaattagct
//
LOCUS       DACGLE010000103         7352 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0534
            SAMEA3304449-rid9560453.denovo.109, whole genome shotgun sequence.
ACCESSION   DACGLE010000103 DACGLE010000000
VERSION     DACGLE010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304449
            Sequence Read Archive: ERR985064
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7352)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7352)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702225.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702225.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 20:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,045
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 27 of 67
            Pseudo Genes (incomplete)         :: 25 of 67
            Pseudo Genes (internal stop)      :: 19 of 67
            Pseudo Genes (multiple problems)  :: 4 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7352
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304449-rid9560453.denovo.109"
                     /strain="VREN0534"
                     /db_xref="taxon:1352"
     gene            complement(58..354)
                     /locus_tag="IXT20_002557"
     CDS             complement(58..354)
                     /locus_tag="IXT20_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanyl-tRNA synthetase subunit alpha"
                     /protein_id="HAQ4860971.1"
                     /translation="MKEYIKNIYFIEETQNIEGSYIEVKTLFVNEDKTKALDIYKKLA
                     SKKTNSFGLILSEYKIKAEESYFYQLLKRWSKLPADFYRKMQIINYQPLAETHA"
     gene            709..1065
                     /locus_tag="IXT20_002558"
     CDS             709..1065
                     /locus_tag="IXT20_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HAQ4860972.1"
                     /translation="MKLEVTKEAQEVLKNKLEADDQLLLDIENGDGPFAESQVSCQLD
                     TAFRLIIVKKDTQTDLSLYSVVADTPVGPIKIKDSAILYMDDPSTLALEPTYNSLQLK
                     GPSGLRKGNLQVVRKD"
     gene            1075..1974
                     /locus_tag="IXT20_002559"
     CDS             1075..1974
                     /locus_tag="IXT20_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
                     /protein_id="HAQ4860973.1"
                     /translation="MGKLAIISDLHVDINKFGESELMQLWQVLQKHKITRLHLAGDTA
                     NKVDRCLAVVEFFVNKGLSTTFHFGNHELADVPNEQMMDHFPDPHFLNERYIALNEQT
                     VLLGMNGWYDYQFSELKDYEAIVRMKRLYWYDRFIQRGKTDLEVNAEMLAAAKNLLDQ
                     LEAKKLEVILSTHFVPKRAFIMYQSAPYERWNKLNAFLGSESFGELLDRYSNIKQVVF
                     GHTHRRFEDKSIGHTIYSCRPFGYYYEWQLTRDFVLKEQLLDSYDPMKLRVLLRAHHP
                     AFQAYQTQHLQEEFEQAMTIVPY"
     gene            2491..3222
                     /locus_tag="IXT20_002560"
     CDS             2491..3222
                     /locus_tag="IXT20_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HAQ4860974.1"
                     /translation="MMKKEIVLTAAMMLFSMVASTTFVSATEVYPKEYNTEGTITFEA
                     GDEGVTPPVDPENPDPNKPVDPSDPPSPGTGGALSIDYGSKFKFGTQKISTADKTYYA
                     AADVMNDGSRKPTYVQVTDRRSTLSGWKLSVSQPEQFKTASGDELVGAQLKFTKGQAV
                     SLVDPTYTPQTVNSELTLTPGGNNTLAINAKSGTGVGTWVYRFGANENENQDAVQLSV
                     PGKSVKLAQQYSTKLVWTLEDTPNN"
     gene            3367..5394
                     /locus_tag="IXT20_002561"
     CDS             3367..5394
                     /locus_tag="IXT20_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lectin"
                     /protein_id="HAQ4860975.1"
                     /translation="MKKRRIVLILSSLLVTNIFPIIGYSLELNQLEHGNRRDEESKLT
                     STFDSLIESNQTQESSEDLSISSSGETTDNVEAEGSDKQGLEDAPISAVELPDDPNII
                     SIDKIFQTPIGTSTSILENGKLLQLNPAERSQRGAIWSNKKISLLSDFTFKSYLYLGN
                     QRDRAGDGMTFTLTGDPRMASNPSQVIGSPGMGIGSYSTRSGQPYIANALSIEFDTYK
                     NSGSSDRMDREITADNRGHGHVAFVTPKPNNNNYSGEHTGVTVAPTFLSNGTWRLLII
                     KWDAEQRSLTYDLEGVGHSSRIIPDLIRQFGSTEVNWGFTSSTGTFFQENAVAVTRLP
                     SNVTSTADVKVNDSDYGAIAEAGGNDKITIRNTLKVEGDFLEYKKMQTSIDLPQELTI
                     PEHILINGNQIDAKDILITGNRVTLNLTDYFIAGDTLIIELETTLQEVIPEKVLTMHF
                     EYLENGNLIEQSNEINLKIAKLKEIDIHVYYKDKDSQIDIAEMKTITGKIGEKYKETP
                     IDIEGYVFDSDSANTEGIFSENTEDIYFYYRKGKLYLSTAPQYVNFGKHKISSRPLSE
                     FGQFSGGLKIIDERAFGSWKLQLKQEKVLTNGDFELPEALSFVTSENTTVIGTSAVTI
                     FESDQKGESDLSSLLDSTAHRGIRIDIPVENQRIGTFEGKLSWILADVPGN"
     gene            5502..6599
                     /locus_tag="IXT20_002562"
     CDS             5502..6599
                     /locus_tag="IXT20_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HAQ4860976.1"
                     /translation="MIKGAIFLRPKQQLFITLLVILGALFNNISVSASAFNFSVTPIT
                     SENQIDKRKTYFDLQLDPDQEVEVKAELRNDTEKEVKIDISVNSATTNSNVMVEYGKN
                     EIEKDMSLIFDLVDYVSYPQIVTLKPKSVQTVAFHVRMPNERFDGVLAGGITFQEIQT
                     EKDQTETKDQSLSIENEYTYIIALLMQQTLNEVAPNLTLHEVKPDQINARNVILANVQ
                     NDQKTYINQVVIETKITKKGHSEVLYQEEKEGLQIAPNTNFSFPTALNGQPLTPGEYH
                     LTMTVLGNENENGKFSRKKGNTTINYTNQWVFEKDFTIDGKVAKELNTTDVTIKKDNN
                     WIYILVASLFLLFILLCILLFVLRKKEEKEK"
     gene            6906..>7352
                     /locus_tag="IXT20_002563"
     CDS             6906..>7352
                     /locus_tag="IXT20_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HAQ4860977.1"
                     /translation="MNYEFFLSKEDHRSYSLLKYLEASSNLSESISNVQEELSLSTFL
                     LKKTIDKLKHDLQKFDLEKNLRVTVSELDICLEINGKCSSKALLSRYITDSLSMKMVL
                     AFFQEKYISVENFAKQHHVSYSVAYKVLQRLKRNLKKYQIFFEKRKL"
BASE COUNT         2555 a         1181 c         1463 g         2153 t
ORIGIN      
        1 atgttcaatc agctgacctt cggtattagc tcaaagcact gatttcacaa cctctattta
       61 ggcatgtgtc tcagcaagcg gttgatagtt gatgatctgc atttttcgat aaaaatcggc
      121 gggaagctta ctccaccgtt ttaataattg atagaaataa ctctcttctg ctttgatttt
      181 atactcgctt aatatcaatc caaaactatt cgtttttttt gaagctaatt ttttataaat
      241 atccaatgct ttggttttat cttcattgac aaagagtgtt tttacttcaa tataagaacc
      301 ctcgatattt tgtgtttctt cgataaaata gatgtttttt atatattcct tcatagtgat
      361 tcctccaaag aagtctttta tttgataagt ttactctacc gaagagttgt taataatccg
      421 tgaacaaaaa gtaattcttt ttccaatagt tgttaaaggt tttttatttt ttatagtatt
      481 ttttgttatt taagaaagga ataaggttaa acaaataaca ggttgctttt tgggtctttt
      541 tccgttcaag aaactgaacg aagaaaaggg aaagttgtct cttttgtgat aagagaaatc
      601 ataataaacg aatgacttac tttccaataa attgagaaca gtcagaacca gacaaattag
      661 ttgcatctga tgaataaagt gattattcta tgacatgagg tgatacagat gaaattagaa
      721 gtgacaaaag aagcacaaga agtattgaag aataaattag aagcagacga tcaactttta
      781 ttagatatcg aaaacggtga cggtccattt gcagaaagcc aagtcagttg ccaattggat
      841 acggcatttc gattgatcat tgtcaaaaaa gatacacaaa cagatctaag tctttattct
      901 gtagtggctg atacaccagt tggaccaatc aaaatcaaag acagtgcaat actttacatg
      961 gatgatccat ccacacttgc tttagaacca acttataata gtttacaatt aaaaggacca
     1021 agcggcttga gaaaaggcaa cttgcaagtt gttcgaaaag actagaggga taaaatggga
     1081 aaattggcga tcatcagcga cctacatgtg gacatcaata aattcggcga atcagagctg
     1141 atgcagcttt ggcaagtttt gcaaaagcac aagatcactc ggcttcatct agccggagat
     1201 acggctaata aagtagaccg ttgcctagct gttgtcgaat ttttcgtcaa taaaggatta
     1261 tctacgacat ttcatttcgg caatcacgag ttggcagatg tgcccaatga acagatgatg
     1321 gaccattttc cagacccaca ttttttaaat gaacggtata ttgctttaaa tgagcagacc
     1381 gttcttttag ggatgaatgg ctggtacgac tatcagtttt ccgaattgaa agattacgag
     1441 gcaatcgtac gaatgaagcg tttgtattgg tatgatcggt tcatccagcg tgggaaaaca
     1501 gatctcgagg tcaatgcaga aatgcttgca gctgcaaaaa atttgctaga ccagctggaa
     1561 gcaaaaaaac ttgaggtgat tttatccact catttcgttc ctaaacgggc atttatcatg
     1621 tatcaatctg ctccttatga gcgttggaac aaattaaatg catttttagg atcagaatca
     1681 ttcggagaat tacttgaccg ttattcaaat atcaaacaag tagtttttgg ccatacgcat
     1741 cgtcgctttg aagataaatc catcggtcac acgatttaca gctgcagacc ttttggttat
     1801 tattatgaat ggcagctgac ccgagatttt gtcttgaaag aacagttgct agacagctat
     1861 gatcctatga aattacgtgt cctcttgcgt gcccatcacc cagcttttca ggcctatcaa
     1921 acacaacatt tgcaagagga atttgaacaa gcgatgacaa tcgttccgta ttgagacagc
     1981 attagtaatg tgtaaaagaa aataccttgt aaaacataag gataagtaag agcagtagat
     2041 ccatttatcg tggatatgct gctttttttg cggaaaataa tcagcgaatg tttatttgat
     2101 cggatgattt gtaactcgca gagtactaga tgcaagcgtg tattcttttt ttgaacgata
     2161 atcacaaaaa aaaatataat atacattttt tatttaatta taattaatat tgattgaagt
     2221 ctaggcagtc aagtcacatt ttgatagata ggagggggac ttacgaatta ttttaaaggc
     2281 tatgacagct gtatttctag attgtgtttg catcaagttg caataaaaac tagaaatcaa
     2341 atcgggctga taatcattta taactaatcg atacatagga ccaaatatag gtctatgttc
     2401 ttttatttca taagtatcgt aaaacagaat tttgctatgg attaatccaa tcgactgata
     2461 tgtttagtaa ataaagaaag aggaatgtca atgatgaaaa aagaaatcgt attaacagca
     2521 gcaatgatgt tattcagcat ggtagcaagt acgacttttg tttctgcaac tgaagtatac
     2581 ccaaaggaat ataacacaga aggaacgatt acatttgaag caggagatga gggtgtaact
     2641 cctcctgttg atccagagaa tcctgatccc aataaacctg tcgatccatc agatcctcca
     2701 agccctggaa caggaggggc attatcgatt gattatggat caaaattcaa atttgggact
     2761 caaaaaattt ctactgcgga taaaacgtat tatgcagctg ctgatgtgat gaacgatggg
     2821 tctagaaaac cgacatatgt ccaagtgaca gataggagaa gtaccttatc tgggtggaaa
     2881 ttaagtgtct ctcaaccaga acagtttaaa actgccagtg gggatgaact agttggagca
     2941 cagctgaaat ttacgaaagg gcaggctgtt tctttggttg atccaacata tacgccgcaa
     3001 acggtgaatt ctgaattaac gttgacaccg ggaggaaaca atacgttagc aattaatgct
     3061 aaatcaggaa caggtgtggg gacttgggtc tatcgatttg gtgcaaatga aaatgaaaat
     3121 caagatgcgg tacagctttc ggtacccggt aaatctgtca agttagcgca acaatattcc
     3181 actaaattag tttggacatt agaagataca cctaacaact agtttactga aaagaactgt
     3241 taaaaataaa tacgtagtca tattgtcttg cgaggtaatc gagcaaaaat tgtcaaaggc
     3301 taggctaaat ttgtttggat acgaatagtt catctaggaa tttaacgatc aggaggaaaa
     3361 gatatattga aaaagaggag gatcgtgctc attttaagca gtctcttagt gactaatatc
     3421 tttccgatca tagggtactc tttggaattg aatcagttgg agcatggaaa tagaagagac
     3481 gaagaaagca aactcacttc gacattcgac agtttaatag aatccaacca gacacaagaa
     3541 agcagtgagg atttaagtat ttcgagtagt ggagagacca ctgacaacgt agaagctgag
     3601 ggttcggaca aacaaggact agaagatgca ccaatttctg cggttgagct gcctgacgat
     3661 cccaatatta tttctatcga taaaattttt caaacaccta tcggtactag cacgtccata
     3721 ttggaaaatg ggaagttact gcagttgaac ccagcagaaa ggtctcaaag aggagctatt
     3781 tggtcaaata agaagattag tttactctcg gattttacat ttaaatctta tttatactta
     3841 ggaaatcaac gagatagagc gggagatggc atgaccttta cattgactgg cgatcctaga
     3901 atggctagta atcccagtca agttattgga tcgccaggta tgggaattgg ctcatattct
     3961 actcgttctg gccaacctta cattgcgaat gcgctctcga ttgagtttga tacgtataaa
     4021 aatagtggtt cttctgatcg aatggatcga gagataactg ctgataatag agggcatgga
     4081 catgtcgctt ttgtcacgcc taaaccaaat aataataatt attctggaga gcatactggc
     4141 gtgacggtag ctcctacatt tctttctaat ggcacatggc gcttgttaat aattaaatgg
     4201 gacgcggagc aaagaagtct tacttatgat ttggaaggtg tcggtcactc ttctcggatt
     4261 atacctgatc tcattaggca gtttggttct acagaagtaa attgggggtt tacttcttca
     4321 acaggtactt tttttcaaga aaatgctgtc gctgtcacgc gacttccatc gaatgtcaca
     4381 tcaacagctg atgtaaaagt caacgatagt gattatgggg ccatcgctga agctggagga
     4441 aacgataaga taactatcag aaatacattg aaggtcgagg gggattttct tgaatataag
     4501 aaaatgcaga ctagtatcga cctcccacaa gaattaacta ttcccgagca tatcttgatc
     4561 aatggaaatc aaattgatgc caaagatatc ttgataaccg gaaatcgagt cacgctaaat
     4621 ctgacagatt attttattgc tggagatacg ttaataatcg aattggagac aactttgcag
     4681 gaagtaattc ctgaaaaagt actcactatg cattttgaat accttgaaaa cgggaatttg
     4741 attgagcaat cgaatgaaat aaacctaaag atcgcaaagc tgaaggagat agatatccat
     4801 gtttattata aagataaaga cagtcagatt gatattgctg aaatgaaaac aatcactggg
     4861 aaaataggag agaagtataa agaaactcct attgatattg aagggtatgt ttttgactcg
     4921 gattcagcga atacagaagg catcttctcc gaaaatacag aggatatcta tttttattac
     4981 agaaaaggca aactttatct ctcaactgct cctcaatatg tcaattttgg aaaacataaa
     5041 atatccagta gaccattaag tgaattcgga cagttttcag gtgggttgaa aattatagat
     5101 gaacgggctt ttggaagttg gaaattacaa ttgaaacaag agaaagttct tacaaatgga
     5161 gattttgagc ttccagaagc tctttccttt gttacctctg aaaatacaac ggttataggg
     5221 acatccgctg ttacgatttt cgaaagtgat caaaagggtg aaagcgattt gtcctctctt
     5281 ttagattcta cagctcatcg tggaatcaga atagatattc ctgtagaaaa ccaaagaatc
     5341 ggaacctttg aggggaaact atcttggata cttgcagatg tcccaggaaa ttaacaggaa
     5401 gagaagaata taacaaaatt attgagtttc aagaagggca caggtaccat aggctcttgt
     5461 gtcccacaga gacaagattt tgttatagtc ttctcttaca tatgattaaa ggagcgattt
     5521 tcttgagacc aaaacaacaa ttatttataa cactcctagt tattttaggg gcacttttta
     5581 acaatatatc agtgtcagct agtgcattta atttttcagt gacgccgata acttcagaga
     5641 atcaaatcga caagagaaaa acttacttcg atcttcaact tgatccggat caggaagtag
     5701 aagtaaaagc ggaattgaga aatgatacag aaaaagaagt gaaaatcgat atttctgtga
     5761 atagcgcaac caccaattca aatgtgatgg ttgaatacgg aaaaaacgag attgaaaaag
     5821 acatgagcct gatctttgat ctggtagatt atgtgagcta tccacagata gtgacgctaa
     5881 aacctaaaag tgtccaaacc gttgcttttc atgtcaggat gccgaatgag cgatttgatg
     5941 gtgtattggc tggtgggatc acattccaag aaatccagac ggaaaaggac caaacagaaa
     6001 ctaaggatca aagcttatcg attgagaatg aatacaccta tattattgct ttgttgatgc
     6061 aacagacact gaacgaagtt gctccgaacc ttactcttca tgaagttaaa ccagatcaaa
     6121 tcaatgcaag aaatgtcatc ttagcaaacg ttcaaaatga tcaaaaaacc tatatcaatc
     6181 aagtagttat tgaaacaaaa attacaaaaa aaggacattc tgaagtttta tatcaagagg
     6241 aaaaagaagg attgcagatt gcgccgaata caaatttttc ttttccaact gctttaaatg
     6301 gtcaaccgct aaccccaggt gaatatcacc ttacaatgac tgttttagga aatgaaaatg
     6361 aaaatggaaa attttcgaga aaaaaaggaa atactacgat caactatacc aatcaatggg
     6421 tatttgaaaa agattttaca atagacggaa aagtagccaa ggaattgaac acgacggatg
     6481 ttacgattaa aaaagacaat aattggattt atatcttagt agcttcactt ttcttattgt
     6541 ttattttatt atgcatactt ttgtttgttt tgagaaaaaa agaagagaaa gaaaagtaag
     6601 gttaagataa caaatagtaa atttttatta ttgtaggatg gttgggctct ttttataaca
     6661 atataaaata taccatttta ttaataaaaa tgcatactag taaatgatat aattctaata
     6721 acaggtttga tcgcgagaaa ggccaaataa aacgctctcc aaaatgttaa aaaaaagaca
     6781 ggacgatgta tattatacaa acaagaatcg ctgacaacag tgactcggat ccaggaaatc
     6841 agttttatcc ccagctagtt ggtgaatgca tggaaagtaa tatacgtaga aagagctgat
     6901 gactaatgaa ttatgagttt tttttaagca aagaagatca cagaagttat tcattactta
     6961 aatatttaga agcaagctca aatttatcag agtcaatatc gaatgttcaa gaagagttgt
     7021 cgctatcgac atttctcttg aaaaaaacga tcgataagct gaaacatgat ttacaaaagt
     7081 tcgatctaga gaaaaatttg cgagtaaccg tcagtgaatt ggatatatgt ctggaaatta
     7141 atggaaaatg ttctagcaaa gctctgttat ctcgttatat aacggattct ttatctatga
     7201 aaatggtatt ggctttcttt caggaaaaat atatctcagt agaaaatttt gctaaacagc
     7261 atcacgtgag ttattctgtg gcttataaag tattacaaag actaaaaaga aatctaaaaa
     7321 aatatcaaat tttttttgaa aagagaaagt tg
//
LOCUS       DACGLE010000104         6643 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0534
            SAMEA3304449-rid9560453.denovo.110, whole genome shotgun sequence.
ACCESSION   DACGLE010000104 DACGLE010000000
VERSION     DACGLE010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304449
            Sequence Read Archive: ERR985064
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6643)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6643)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702225.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702225.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 20:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,045
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 27 of 67
            Pseudo Genes (incomplete)         :: 25 of 67
            Pseudo Genes (internal stop)      :: 19 of 67
            Pseudo Genes (multiple problems)  :: 4 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6643
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304449-rid9560453.denovo.110"
                     /strain="VREN0534"
                     /db_xref="taxon:1352"
     gene            complement(1..859)
                     /locus_tag="IXT20_002564"
                     /pseudo
     CDS             complement(1..859)
                     /locus_tag="IXT20_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            1102..1557
                     /gene="tnpA"
                     /locus_tag="IXT20_002565"
     CDS             1102..1557
                     /gene="tnpA"
                     /locus_tag="IXT20_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002366027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HAQ4860978.1"
                     /translation="MSNDDKSLAYTRWNCKYHIIFTPKYQRKVIYGQLRKDLGKILRK
                     LCEMKDVEIIEAHAMLDHIHMLVRVPPKICISSFMGYLKGRSAVLIHEQHANLKYKYG
                     NKSFWSKRYYVSTVGLNQKTNEKYIREQEADDRIRYIISKREYVEPFKK"
     gene            1822..1920
                     /locus_tag="IXT20_002566"
                     /pseudo
     CDS             1822..1920
                     /locus_tag="IXT20_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            complement(2143..2373)
                     /locus_tag="IXT20_002567"
     CDS             complement(2143..2373)
                     /locus_tag="IXT20_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="resolvase"
                     /protein_id="HAQ4860979.1"
                     /translation="MDEAMDHPLLNKFIKDLILQIFSMIDEQEPTESKRHRAQGIIKI
                     AKRMFSIKGVLSYTVLIQKIPNHLVYKNNVED"
     gene            complement(2603..3421)
                     /locus_tag="IXT20_002568"
     CDS             complement(2603..3421)
                     /locus_tag="IXT20_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAQ4860980.1"
                     /translation="MSYIYLIKENYPFLTKSEKKVADFILNSGKTIIYSTMSDIKKNT
                     KVGDATIIRFCQKIGFSGFSDLKIEIAKEDFSQKKEHPTSVKYYDEIANSLTEALYST
                     IRLLDEEKLNEAIKMITQSKNIYIFGVGSSGNTSLDLENMFLRVGVQAKAVLDPHFQA
                     QVASLLTVNDLVIIFSLSGKTKDTYDSLKIAKKNGAKIIAITNYIHSPIGKSADLVLQ
                     TAIEEFLNGGSLAGKISQLYICDLLVHGYEQNNNIDSVELREKVLRSIIDKSIE"
     gene            3582..4271
                     /locus_tag="IXT20_002569"
     CDS             3582..4271
                     /locus_tag="IXT20_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HAQ4860981.1"
                     /translation="MDKKRFLDLISKGLIVSCQALPGEPFYKEEESLMPYFALAAQRA
                     GAVGIRANSVRDIIEIRKCVSLPMIGIIKRDYPPESPFITATMREVDELVETGVEVIA
                     LDCTLRNRHDGLSINSFITKIKEKYPHQLFMADISTFEEGKNAYKAGIDFVGTTLSGY
                     TEESKKQDGPDIDLIKKLVEEGICVIAEGRIHTPEQAKEIKKIGVAGIVVGGAITRPQ
                     EIASRFVKALS"
     gene            4285..5787
                     /locus_tag="IXT20_002570"
     CDS             4285..5787
                     /locus_tag="IXT20_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010759843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ4860982.1"
                     /translation="MFKKFSQLGRAFMLPIAILPVAGLLLGLGGALTNESAINAYPFL
                     DQPWLHTILSIMSYAGNAVFANLALIFAIGIAVGLANGDKGTAGLAGGVAYLVYTATI
                     SGFLELFSAKDATLDTGVVGSIVVGGTVAFLHNRYRKIELPQFLGFFGGSRFIPIVSS
                     LAAIIIGSFFYLIWPPIQNGLVVAGEQIAQMGSLGTFLYGFLLRLTGAVGLHHTIYPL
                     FWYTSLGGTETVAGSTIAGAQNIFFAQLADPNHTGLFTYGTRFFAGRFATMMFGLPAA
                     CYAMYRAIPKKNRKKNGGLYFSGALTSFLTGITEPVEYMFLFVAPWLYVIHAFLDGLS
                     FYFADILNIRVGNSFSGGLIDFLLFGVLQGNDKTNWLKLIPFGIAWAFIYFFVFTFFI
                     KKFKVAIPGMEEESEVHSEKVINKNTSLHEESLIIIEALGGKENIESVEACATRLRVA
                     VTNGEIIQKQTIKDTGATAVFEVKGGIQAVFGGKADLLSQEINQILGKDN"
     gene            5800..6276
                     /locus_tag="IXT20_002571"
     CDS             5800..6276
                     /locus_tag="IXT20_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HAQ4860983.1"
                     /translation="MFEIFKKKKQETFYAPCSGKIISITEVNDPVFSQKLLGDGFAVI
                     PDGSKIYAPLAGRVTNIFPTKHAINIKSNSGIDYLIHIGIDTVELQGEPFSICVQEND
                     EVTPETSLVDVDFDKIIKSGKDTSVIVVFPEKNQFNDLIIDNGRIAHGNSCGKITF"
     gene            complement(6373..6483)
                     /locus_tag="IXT20_002572"
                     /pseudo
     CDS             complement(6373..6483)
                     /locus_tag="IXT20_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         2172 a         1033 c         1203 g         2235 t
ORIGIN      
        1 acttgtcctc gataagaatt gtagcgccgt gatttacgtg tgtatgtcgt tacttttatt
       61 cctagcttct tgatcaggcg ctgaactttc tttttattga tagagaaacc gcggtttctt
      121 agttcattgg tcatccgtaa acagccataa tccttgtgct tctcccgaac tttaagcatt
      181 tccgcttcaa tctcttgatc tggattttta cggtccaatc gtttttgcca gtacataaag
      241 attgccttag ggaactttaa agtttctaga agctcaacta atttaaacgg tcctcggagg
      301 cttgcgatga ttcgtgcgat tgcttcattc gtcgttgttg ggcttcctgt tttcgcagct
      361 tcctcaattc ttttaaaaag gcattttcaa tttgaaggga acggactcgt ttctccaact
      421 cctcgatgcg ttcacgttct ttagacgttg ctttagttgt agattttttc ttatttttgg
      481 gttcattttt cttagacaag ataggtggac atcctttcgt ttttgagagt ccatcgatcc
      541 caccctcacg gaattgacgc atccaactag cgatcaaatt agggttgttc atgttaagtg
      601 tgttggcaac ttcacgatag gacagctcac ttgttagata taactctatc gcatccagct
      661 taaattgaac agaatatttt ttcttttgtc gctttcttac tagaccctct tcaccaaatt
      721 cttgataggc attgatccag cgcttcactt gtgatggagc cttaatggaa tatttctttg
      781 ccagataatc acttccgcct ttaccatcca agtaatcatg aacgatcttt aatttaagtt
      841 caaaagtata cttagccata aaaaataccg acctccaaaa gttagatttt ttggtctaac
      901 ttttgggggt cggtacaaaa cttaaaagtt ttagaccctt ttattttctt aatgacttca
      961 gtcagttttg tgcgtaaagc gtgcgaaaca agaaaaccgc gcgtttctac gcgtggaaga
     1021 agtatcttta gcgataaaaa ctcctttcga tatactagtg agtggctacc aaccgctcat
     1081 cagatcgaaa ggagtttttt catgtcaaat gacgataaaa gtttagcata tactcgttgg
     1141 aattgtaagt atcatattat ttttacacca aaatatcaac gaaaagtaat ctatgggcaa
     1201 ttacgaaaag atcttggaaa aatacttagg aagttgtgcg aaatgaaaga tgtggaaatc
     1261 atagaagcac atgcaatgct agatcatatt cacatgcttg ttcgagtacc accaaaaata
     1321 tgtatatcca gttttatggg gtatctgaaa ggtcgtagtg cggtactaat ccacgagcag
     1381 cacgcaaatc tcaaatataa atacggaaac aaatcctttt ggtcgaagag atactatgtg
     1441 agtacagtcg gattgaatca aaagacaaat gaaaaataca tccgtgaaca agaagctgat
     1501 gatcgcattc gatatattat aagtaaaaga gagtacgtag aaccatttaa aaaataaaga
     1561 gaagtaagtg agtgaagatg agcagttggc ggtcttttaa aaatcccctt ttaaggggca
     1621 gcgagtgtcg accccttcta gggggattaa aaatacaccc gtcctcacgg gtgtatgggt
     1681 tactgataga cttgatttta actggtgtca gaaacttatg tccagaaagg gtgatgaaat
     1741 tttggcaatt tacggctatt caagaattag cacaagcaac caagattaca aaacacaaat
     1801 tcaaacatta gaaaatgctg gcgcagaaat aatcttccta gaaaatcact ggattatcga
     1861 acacctttgg aagtattttt gagttacgta agtattgatg atctgtctaa cttaatttga
     1921 caattaagag tatatttact tcagatggta aggcaacaaa ataaaataca ataggcagat
     1981 gaactaataa ataaaatctg tattaaatac agataaatta atataaaaat caaaaggatc
     2041 ataaactttt aacaagtgat gaaatgggtg gaaattgatt cttatagaca gtttgtctca
     2101 taacattgaa gtattttgca attttagcat tgaaacactt ttttaatctt caacgttgtt
     2161 cttataaaca agatgattgg gaatcttttg aattaacact gtatagctta gaacaccctt
     2221 tatagaaaac attcgcttag caatcttaat aattccttgt gcccgatgtc gtttagattc
     2281 agtaggttcc tgctcatcga tcatggaaaa aatttgaagg atcagatcct taataaattt
     2341 atttaagaga ggatgatcca ttgcttcatc catactcgat aaactagtga taatgagcta
     2401 cacgtttttc tttcaagttg atcaatagct tccacgataa aaatatcttt agataaaatc
     2461 aagaaccgta ggaactacag ggatatttgg tcaataagag ccaccgctat tagtaaaaaa
     2521 taagctatca agtatgctct catcccaaga attgcctact tcaatcgtta aaaaccgtta
     2581 ggtttagcta agtgggggta gttcactcaa tacttttatc aataatagag cgcaaaactt
     2641 tttctcttaa ttcaactgaa tcaatattat tgttttgttc ataaccatga actaatagat
     2701 cacaaatata aagttgtgat atttttccag ctaaagatcc accattaaga aattcttcaa
     2761 tggctgtttg taaaactaag tctgcagact ttcctatagg tgaatgaata tagttagtta
     2821 tagcaataat ttttgcgcca ttttttttag ctattttcaa tgaatcataa gtatctttcg
     2881 ttttcccaga taaagaaaaa atgattacta aatcattaac tgttaataaa gaagcgactt
     2941 gggcttgaaa atgagggtct agtacagctt ttgcttgaac tcctacgcga agaaacatat
     3001 tttctaaatc aagactagta tttccactag atcccactcc aaatatatat atgttttttg
     3061 attgagtgat cattttaatg gcttcattca atttttcttc gtctaaaagc ctaatagtgg
     3121 aatagagagc ttctgttaaa ctattagcaa tttcatcata gtactttaca ctagtcggat
     3181 gttctttttt ttgactaaag tcttcctttg caatttcaat ttttaaatca gaaaaaccag
     3241 aaaaaccaat tttttgacaa aagcgaataa tagtagcatc gccaactttt gtgtttttct
     3301 taatatcact catcgtacta taaataatag ttttcccaga atttaaaata aaatcagcga
     3361 cttttttttc agacttcgtt aaaaatggat aattttcttt aattaaatag atataactca
     3421 tacaaactcc tctacaattt caacttttct ccattatact ccaaaataat aaaaaaaact
     3481 cctaaatact ccatttttat ctttactttt atgaaagcgg atgctatatt gtttttggag
     3541 ttgttatcca aaaataataa aaaattggag tgtttaggat aatggataaa aagaggtttt
     3601 tagatcttat ttcaaaaggt ttgattgttt cctgtcaagc attacctggt gaaccatttt
     3661 acaaagagga agaaagttta atgccttatt ttgctttggc tgctcaaagg gctggagcag
     3721 ttggtatacg tgcaaattct gttcgagata ttattgaaat tagaaagtgt gtatcattac
     3781 caatgattgg aattattaaa agagattatc caccagaatc tccattcatt actgccacta
     3841 tgagagaagt agatgaatta gtagaaaccg gtgttgaggt aatcgctctt gattgcactc
     3901 ttagaaatcg acatgatgga ctatctataa attcttttat tactaaaata aaagaaaaat
     3961 atcctcatca gttatttatg gcagatattt ctacatttga agaaggcaaa aatgcatata
     4021 aagcaggaat agattttgta ggaactactt taagtggata tactgaggaa agtaaaaaac
     4081 aagatgggcc agatatagat ttgattaaaa aattggtaga agaagggatt tgtgtaattg
     4141 ctgagggaag gattcatact ccagaacaag caaaagagat aaaaaaaata ggagttgcag
     4201 gtattgttgt gggtggtgct attactcgac cacaggaaat tgcatcaaga tttgttaaag
     4261 cattaagtta gggggagaaa taaaatgttt aaaaaatttt cacaattagg acgcgctttc
     4321 atgttaccaa ttgctatatt accagtagcc ggattactat tgggactggg aggagcgtta
     4381 acaaatgaga gtgctataaa tgcttatcca tttttagatc aaccgtggtt gcatacaatt
     4441 ttaagtatta tgagttatgc tggaaatgca gtttttgcaa acctagcact aatttttgca
     4501 attggtattg ctgtaggact tgcaaatgga gataaaggaa cagctggttt agctggtgga
     4561 gtagcttatc tagtatatac tgcaactatt agtgggtttc ttgaactttt ttcagcaaaa
     4621 gatgcaacac tagatacagg tgtagtggga tcaattgttg ttggtggtac tgttgcattt
     4681 ttacacaatc gttatagaaa aattgaatta ccacaattct taggtttttt tggaggatca
     4741 agatttattc cgattgtttc tagtttagct gcaatcatta ttggttcttt cttttattta
     4801 atttggccac caattcaaaa tggtttagta gttgctggtg aacaaattgc ccaaatgggt
     4861 agtttaggaa cttttttata tggtttttta ttgcgtttaa caggtgctgt tggactacac
     4921 cacaccattt accctttgtt ctggtataca tctttaggtg gtaccgaaac tgtagctgga
     4981 tcaacaattg ctggagccca aaatattttc tttgcccaac tagctgatcc aaaccacact
     5041 ggtctgttta cttatggtac tagatttttt gcaggaagat ttgcaacaat gatgtttgga
     5101 ttaccagcag catgttatgc aatgtatcgt gcaattccta agaaaaatcg taaaaagaat
     5161 ggtgggcttt attttagcgg ggcccttaca tcatttttaa ctggaattac tgaaccagtt
     5221 gaatatatgt ttttatttgt tgctccttgg ctttatgtga ttcatgcatt cttagatgga
     5281 ttatcttttt attttgctga tatcttaaat attcgtgtag ggaattcctt ttcaggtggt
     5341 ttgatcgact ttttattatt cggtgtactt caagggaatg acaaaactaa ttggttaaaa
     5401 ttaattcctt ttggtattgc atgggcattt atatatttct ttgtttttac attctttatc
     5461 aaaaaattta aggtagctat tcctggaatg gaagaagaat cagaagttca ttctgaaaaa
     5521 gtaataaaca aaaatacatc attacatgaa gagtctctta tcattattga agctcttggt
     5581 ggaaaagaaa atattgagtc tgttgaagct tgtgcaactc gtttgcgtgt tgctgttacg
     5641 aatggggaga ttattcaaaa acaaacaata aaagacactg gtgcaactgc tgtcttcgaa
     5701 gtgaagggtg gcatccaagc tgtttttgga gggaaagctg acttattaag tcaagaaatc
     5761 aatcaaatat taggaaaaga taattaggag gtttttaata tgtttgaaat tttcaaaaag
     5821 aaaaaacaag aaacttttta tgcaccttgt tccggaaaaa tcatttccat cacagaagta
     5881 aatgatcctg tcttttcaca aaaattacta ggtgatggtt ttgccgtcat acctgatggt
     5941 tcaaaaattt atgctccatt ggctggtcga gtaacaaata tatttcctac taaacatgct
     6001 attaatataa aaagcaactc aggcattgat tatttgattc atattggaat tgatacagtt
     6061 gaattacaag gagagccttt ttcgatttgt gttcaagaaa atgatgaggt tactcctgaa
     6121 acctcgttgg ttgatgtgga ttttgacaaa ataataaaat ctggtaaaga tacttctgtt
     6181 atagttgttt ttccggagaa aaatcagttc aatgatttaa taatagataa tggtcgaatt
     6241 gctcatggga attcatgtgg aaaaatcact ttttaaaaaa gtgtatataa aaagctataa
     6301 aataagctaa ataggatatt tgtcaaatga agttggtgaa cagaaagaac tgttttcttt
     6361 ctgttcattt ttttatttta ttagtaatga ttccccatgc caaatcatga tccgtaattt
     6421 aaagttataa aagcttcgaa atccattggc atttccttgg aataccttga tatgattatt
     6481 caaatataaa atatagagag aagagagaga gggaagatat ttataaaggg caaagttatg
     6541 tttactcaac ttaggaaata gaaatagatc aatttatatt ctcttgtcaa tcgaatatga
     6601 accttagcac tatataccaa atatagtaaa atgctgaaag atg
//
LOCUS       DACGLE010000105         6513 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0534
            SAMEA3304449-rid9560453.denovo.111, whole genome shotgun sequence.
ACCESSION   DACGLE010000105 DACGLE010000000
VERSION     DACGLE010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304449
            Sequence Read Archive: ERR985064
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6513)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6513)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702225.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702225.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 20:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,045
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 27 of 67
            Pseudo Genes (incomplete)         :: 25 of 67
            Pseudo Genes (internal stop)      :: 19 of 67
            Pseudo Genes (multiple problems)  :: 4 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6513
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304449-rid9560453.denovo.111"
                     /strain="VREN0534"
                     /db_xref="taxon:1352"
     gene            complement(95..1225)
                     /locus_tag="IXT20_002573"
     CDS             complement(95..1225)
                     /locus_tag="IXT20_002573"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HAQ4860984.1"
                     /translation="MSKNRPFTVDHVGSFLRPQSIKEARKKVQEGLLSKEELRAIEDQ
                     EIKKLVEDQKAVGIKGITDGEFRRGFWHLDFLEELNGIEGYVPETGYNQTFHGKAAPA
                     YNIRVVDRLSFNPNHPFLKDFAFLKEVVETDDTFVAKATIPSPTMILRQEILSNDGSS
                     KIKEIYPDLSDFYEDLSSTYKAAIHAFYEKGCRYLQFDDTNWAFLADQTKREALLQKG
                     IDPKEMAHICTEIINNALEDKPEDLTITTHICRGNHASSWLFSGGYEPIAEELFSTNY
                     DGFFLEYDSDRAGDFTPLRHWSNKESKVVLGLVTSKFPDLEDTEKIKERIKEAQQFVP
                     LENLSISPQCGFASTEEGNHLTEEEQWKKIQLLQQVAKEVWL"
     gene            complement(1874..3076)
                     /locus_tag="IXT20_002574"
     CDS             complement(1874..3076)
                     /locus_tag="IXT20_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAQ4860985.1"
                     /translation="MPKIKNIYQDKKTKKWFFRAYLGIDENGRKVQKTKRGYASQKDA
                     KIAYDKYMETHDFNKTIPNQLCSTNQMTFEEFYKDRFVKWYERQVKSQKYENAQFIFE
                     KRMQFFYHFRVRDITSHDIEDWLFELSQTESRNSRKKVNGETLSKSYINRIRGHLKIV
                     LDRAVKEGVIAKNPVEDVSSLPSENKKVEFWEHSEFQKVMNELKDDKIQTHHRKIVYE
                     MLFYTGIRIGELEALSWSNVDFEKNTITIEKTLIYNTKDDWYFSTPKTKCAYRTIGLG
                     KKLSLKLRQWYDLQKRIGNFEYVAQLDGTFTPPYSFANWLKEAAKKAGVKPIKLHALR
                     HSHVAFLIEQNIQPLSIQERLGHANIQITLGTYGHLYAKSDQQVVSAIDNVQEGAILS
                     KNDNLLPV"
     gene            complement(3136..3339)
                     /locus_tag="IXT20_002575"
     CDS             complement(3136..3339)
                     /locus_tag="IXT20_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HAQ4860986.1"
                     /translation="METKCDFMVNRAILIEMGFKPSQAARMIKESKAYLARIEGIDFY
                     NNRQVGVVPSRVIEHLFHIQVAE"
     gene            complement(3481..4713)
                     /locus_tag="IXT20_002576"
                     /pseudo
     CDS             complement(3481..4713)
                     /locus_tag="IXT20_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173388.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
     gene            4876..5688
                     /locus_tag="IXT20_002577"
     CDS             4876..5688
                     /locus_tag="IXT20_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002406512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ4860987.1"
                     /translation="MEIDKQAVGNRIRQIRQELKLSMEKFGKLIGDLPRSTVNNWERG
                     INLPKTETLHQIAEIGHVTNEYLLYGDQENQYILEMLQKKAGKLDPKIEGLIVDEIKH
                     ADLVSEKEMDRMIEFFITNLIPPTEQDQFTFQCIDEKKQLYLGSTNFGKEAQLYLQHD
                     NQNHILHMMPFTFSNFSVDRILVYLANQKSYSYFGKHLPKELGEKAILLYSINQLTSD
                     VRIAPLVYSEETASYQYTEDNQYLLEQQYLYLPFVMEVEKERLLNAAYPSSK"
BASE COUNT         1967 a         1324 c         1016 g         2206 t
ORIGIN      
        1 aaataatata aaaggtaaat aaaaaagagt ggatttctcc actccatgaa aagccgactt
       61 tttgggacgc ttttttttgc caagcctcac atttttatag ccaaacttct tttgcgactt
      121 gctgcaataa ttggattttc ttccattgtt cttcttctgt taaatggttt ccttcttctg
      181 ttgaagcaaa cccacattga ggactgatac ttagattttc caatggtaca aattgttgtg
      241 cttcttttat tcgttctttt attttctccg tatcttccaa atctggaaac ttagaagtaa
      301 ctaatcctaa aacaacttta ctttctttgt tactccaatg tcttaaagga gtaaaatctc
      361 ctgctcggtc agaatcgtat tccaaaaaga aaccatcata atttgttgaa aacaactctt
      421 ctgcaatggg ttcatatcca ccggaaaaca gccaagaaga agcgtgattc ccgcggcaga
      481 tatgtgttgt gatcgtcaaa tcttctggtt tgtcttccag tgcgttattg ataatctctg
      541 tacaaatatg ggccatttct ttcggatcaa ttcctttttg aagaagtgct tctcttttag
      601 tttgatcagc tagaaacgcc caatttgtat catcgaattg caggtaacgg caaccttttt
      661 cataaaaagc atgaatcgct gctttgtaag tagaggacaa gtcctcataa aaatcagata
      721 gatcaggata aatctccttg atttttgaag aaccatcatt agataggatc tcttgtcgta
      781 aaatcatcgt gggactaggg atcgttgctt ttgcaacaaa agtatcatct gtttccacta
      841 cttctttcag aaaagcaaaa tcttttaaaa atgggtgatt tggattgaaa gataatctat
      901 ctactacccg aatattataa gctggtgctg cttttccgtg gaatgtttga ttatatccag
      961 tttctggaac atagccttcg ataccattta gttcttctaa aaaatcaaga tgccaaaatc
     1021 cacgccgaaa ttcaccatct gtaattcctt tgatgccgac tgctttttgg tcttccacta
     1081 atttttttat ttcctggtct tcgattgcac gaagttcttc ttttgaaagt aagccttctt
     1141 gaactttttt tcttgcttct ttgatcgatt gcgggcgtaa gaaacttccg acatgatcta
     1201 ctgtaaatgg tctatttttg ctcatttcac tcactctttt ctttgttttt tgtaaaaaaa
     1261 aatcctttta ctagcaaata ctagtaaaag gacgattata ctcgtggtac cacctttttt
     1321 cagaaaaaac attctttctc tcagcctgac aataaaatca gtggtcttgg taacgaaaga
     1381 ctgttcgttt ttgcctacct ttcgacaaaa aatcctctaa ggccattttc gacagtcaac
     1441 ttctagcttt ttacaccaac caaagcctct cttgaaccat tttctgtcta ctttccttct
     1501 catcggatac tattagaata aaattagaat agcatgtttg aaagaaaagt caatagggaa
     1561 taagaacaaa caaaaactaa tttgttattc ttagtaattt gtttgtggaa tacaataaag
     1621 cttttctaat atgataattg ttcagtcgtc ctttcatctt agatcacttc cttaaaatga
     1681 tcatacccgt ttcaaccaca taaaaaagga cttaattttg aaaaatttcc catcaaaact
     1741 aagtcctttt tattatagtt atgcaaattt gcttattttc tcgctatttc cgatacatct
     1801 tgaacaggag caattcgact gaaaacactg gtgcaaaagc atttatttgt cttatggtag
     1861 aaccgtggaa aactcagact ggtaataaat tgtcattttt tgaaagaata gcgccctctt
     1921 gaacattatc aatcgcactt accacttgtt gatctgactt tgcatataag tgaccgtaag
     1981 ttcctaaagt gatttgaata ttcgcatggc ccaatcgttc ttgaatagat agcggttgaa
     2041 tattctgctc aattagaaat gcaacatgag aatgtcgtag cgcgtgaagc ttaatcggtt
     2101 tgacacccgc ttttttagca gcttctttta accagttcgc aaaagaataa ggtggcgtaa
     2161 atgttccgtc caactgagca acatattcaa aatttccaat acgtttttgc aagtcatacc
     2221 actgtctgag ttttaaggaa agcttcttcc ccaacccaat cgttcgataa gcacatttag
     2281 ttttgggagt cgaaaagtac caatcatctt ttgtattata aatcaatgtc ttctcaatcg
     2341 ttatcgtgtt tttctcaaaa tctacattcg accaagacaa agcttcaagt tctccaatcc
     2401 gaatgccagt ataaaagagc atttcataca caatcttccg atggtgggtt tgaatcttat
     2461 catccttcaa ttcattcatc actttttgaa actcactgtg ttcccaaaac tcaactttct
     2521 tattctcaga aggaagcgaa gaaacatctt caacgggatt tttagcaatc accccctctt
     2581 taacagcccg atccaataca atttttaggt gccctcgaat ccgattgata taagattttg
     2641 ataacgtttc gccattaact tttttccgtg aattacgtga ctctgtctgg cttagttcaa
     2701 ataaccaatc ctcaatatcg tgacttgtaa tatctcgaac acgaaaatga tagaaaaact
     2761 gcatacgctt ttcgaaaatg aattgcgcat tctcatattt ttgacttttg acctgtctct
     2821 cgtaccattt tacaaaccta tccttgtaaa actcttcgaa agtcatttga ttggtagaac
     2881 acagttgatt aggaattgtc ttgttaaaat catgtgtttc catgtacttg tcataagcaa
     2941 ttttggcatc tttttgtgaa gcataacctc gtttagtctt ctgaaccttt cttccatttt
     3001 catctatacc tagatacgct cggaagaacc atttttttgt ctttttatct tggtaaatat
     3061 ttttaatttt tggcatcgtt ccattcctcc ctgtacttga gaaaagctac tttaatagta
     3121 ccgcgcttgt actgtttatt ccgcaacttg aatgtgaaaa agatgttcaa ttactcggga
     3181 tggcaccaca ccaacttggc gattattata aaaatcaata ccttcaatcc ttgcaagata
     3241 tgccttgctc tctttaatca ttcgtgcagc ttgactaggt ttaaagccca tctctatcaa
     3301 aattgcacga tttaccatga aatcacattt tgtttccatt ctctcacctc ctgattcttt
     3361 actcctttta aaataatcct ctttataatc tattgtttct cattcgatgg ttgtgttata
     3421 ctcgtcttgt cgaggcggag tacgttgcac aacttgttgt gtaagcagta ctttgctttt
     3481 ttagtagctt gttttctcat ttgatatact ttgtaatttt cgttcataat actcaacctc
     3541 ctcctgataa tactccgggt ctgccttcat attttggaga agtttgatct gtcttggatt
     3601 catttctcct tgttcaataa tcaaaggcaa caaggaagaa tgaaacgttt gatcgacctg
     3661 tgcaataaaa actaatgatg gtgaaacacc atgaattaac caactcatag ttttctcaaa
     3721 actttgagga attgtatcta aagaaatggt gattgcgcca taattgctaa taaaggctgc
     3781 ccatttggca tcaacttttg cacctagatc atttggatca cggtcataaa aacaaattgc
     3841 tccattaagt aactcaaaga acagtttggt taaatcatgg gtttttgaaa taatctttgc
     3901 caagctttgc gctttctctt ttcgataccg tagttcaaag cgattcttta cttttgcctc
     3961 ttctaaagga attcctcgtc gcttccgttg ttcatggtcc ttctgataaa agcaaaaacg
     4021 acactgactc ttctttgagc ctaaatacaa ggtctcacca tttgattcat tgtttatgat
     4081 atccgtaccg ccatgagatt cactcacttg aaaagtggtc cacacatggc ctagtattac
     4141 cttatgctta agttctggaa tgctgagact ccccacaaaa tcatctaaag taaaatcaat
     4201 ccgtgtaaag ttcccctgat aatctaatac cttacgaaaa aactgttgcc aatttgtttt
     4261 tcgtgttttt agccatcttg ctaagtgttt acagccttga cctgaaaact ccatcacagt
     4321 tcctttaacc gcatcgtcaa tggaataccg aatcgtgatt acttgattcc acacgtattg
     4381 gccaatatag ccctttggcg cataatccag ttcctcaaaa cgttcaatgg gcatttcaat
     4441 aatctcttca attagcttta aatgttgatt cgagaaaaag tgaatggtta gataatcaat
     4501 caaaacacgg ttaactggaa cgacctcttc ttgcaaacta tccaacacaa gattcaattt
     4561 ttcttcactt aattgtctcg ttcctttttc tactttcgat aataactatc tcgaaatccc
     4621 cactttttct gctaaatatc gttgtgtcca ccccctttct tctcgagaaa ctcgaatggc
     4681 ttcaccgaga tcatgttgat taacttggtt catcgacaaa cctccttctg ctatttactt
     4741 gtcaaagaac ctattgaact tattttaagt acatttttat tgtacgcctg tatttatttt
     4801 aagtcaatat gttttgactt tttttaagta cacacgtata ctaaatttaa gaacttatcc
     4861 tgaggaggag ttttcatgga gatagacaag caagcagtcg gcaatcgaat tcggcagatt
     4921 cgccaagaat taaagttgag tatggagaaa tttggcaaac taattggtga tttaccgcga
     4981 agtacagtca ataactggga acgtgggatc aaccttccaa aaaccgaaac ccttcaccaa
     5041 atcgcagaga taggtcatgt caccaatgaa tatcttttgt atggtgatca agagaatcaa
     5101 tacattttag aaatgctcca aaaaaaggca ggtaagcttg atcctaagat tgaaggtctc
     5161 atagtagacg aaatcaaaca cgctgacctt gttagtgaaa aagaaatgga tcgaatgatt
     5221 gaatttttta ttacaaatct cattccacca actgaacaag accagtttac tttccaatgt
     5281 atcgatgaaa aaaagcagct ttatcttggt tcaactaatt ttggtaagga agcccagctc
     5341 tatttgcaac atgataatca aaatcatatt ctgcacatga tgccctttac cttctccaac
     5401 tttagtgtgg atcgcatact cgtctattta gcaaatcaaa aatcctattc ctattttggg
     5461 aaacatttac ccaaggaact aggtgagaaa gccatccttc tatactcaat taatcaattg
     5521 accagcgatg tgcgaattgc cccgttggtc tattcagaag aaaccgcttc ttatcagtac
     5581 acagaggaca atcaatatct actagaacag caatatcttt atctaccatt cgtgatggaa
     5641 gtcgagaaag aacgcctgtt aaatgcagcg tatccatcat caaaatgagg tgacaaaatt
     5701 gataagcacg tgacaaagaa aatactgcta ggacactccc caatgggtag ttgtcacagt
     5761 ttcctgttct ttgttagccc cctgttagaa ccgggggctt tattcctgca gtccggctac
     5821 gccttcctgc aatagacaaa actccagatc gggtaattcg cttgtggcga aacccgacct
     5881 ggagtttgtc tctgtatttt gaaccctaaa aattaatcaa acagactgat attctcataa
     5941 tcagcgcgaa ataagccatt aagtacagtt ttcctaccta tttttgataa cccgctggtt
     6001 agttaatatt ccttttccga gagagataaa tgattggtta aatctatata catctcaacc
     6061 tttaaaaatg ttttcgaaat acttcatcag tacatgtcat acaaattacc agtgcattct
     6121 caaattctag ttctttaatt gatttcagtt gaggagcagt tacttttggc ggaaatttcc
     6181 atttttgtcc aaacctcttc ccccgatgaa atttctttgt tgcaattatt gcaatgaaaa
     6241 atttttgttt caaaaatata cataattact tcctccaata aaaactatct acctctcaaa
     6301 ttatttttct ctaataagaa aaacagaaaa ggtaataaga tacaacagtg atgattccta
     6361 ataaagaaaa gactacaata atatttttct aacttatcgc cttcaaagaa atagtttctt
     6421 atttttcact aaaataaaaa atttattgca aaaaaaatag aagtgacaaa attatcaatc
     6481 atttcaaggt aatataacta cctgagttta tta
//
LOCUS       DACGLE010000106         6421 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0534
            SAMEA3304449-rid9560453.denovo.112, whole genome shotgun sequence.
ACCESSION   DACGLE010000106 DACGLE010000000
VERSION     DACGLE010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304449
            Sequence Read Archive: ERR985064
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6421)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6421)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702225.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702225.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 20:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,045
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 27 of 67
            Pseudo Genes (incomplete)         :: 25 of 67
            Pseudo Genes (internal stop)      :: 19 of 67
            Pseudo Genes (multiple problems)  :: 4 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6421
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304449-rid9560453.denovo.112"
                     /strain="VREN0534"
                     /db_xref="taxon:1352"
     gene            232..1107
                     /locus_tag="IXT20_002578"
     CDS             232..1107
                     /locus_tag="IXT20_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ4860988.1"
                     /translation="MKESASCFVLSYRGGELTVYVMKKLNHNAVLIDDDGIEKIAMGK
                     GIGFSAKVSKPFDVEDADKLFVLDSKEKIKRFSEIVSQIPIEFMDFAEEVIELITARI
                     SSPLDSNIYITLTDHIYFAIQRKREDRDVTAIMLPEMRFLYPNEYDIAVEVVERINHR
                     YQTELGSNEVGFITMHIINAELGEPNSLKSLKIVEMTTTILSHLEKSYSNVYDKESLT
                     YHRLMVHIKFLVKRLIYQEETPVENIGFFTETFKHSKAYCTALKIKDIIEQQFQLAIQ
                     ENEIIYLAIHLARIY"
     gene            1211..1975
                     /locus_tag="IXT20_002579"
     CDS             1211..1975
                     /locus_tag="IXT20_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate deacetylase"
                     /protein_id="HAQ4860989.1"
                     /translation="MGKVIFNSDDFGYSHGVNYGIMDAYQRGILTSTTLMANMPGFEH
                     AVKLRKEMPRLGVGVHLTLTCGKPLLKTVDTLIESGEFRSLSYYQHPFNIDKDQLYQE
                     WNAQIQKIYHAGIIPTHLDSHHHTHTLDENQEVVVTLAKKYDLPVRSNFERKDEVRHV
                     DYFEPCFDVVGETDEKKRQINTSVEEYLASLLMILREDKTIEVMCHAAYLDQELLNGS
                     SFVYPRTHQVEFLIHSAFAQKVKNDPLIELVTYEDI"
     gene            1992..3404
                     /locus_tag="IXT20_002580"
     CDS             1992..3404
                     /locus_tag="IXT20_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ4860990.1"
                     /translation="MNVKSNVQKLGRAMLLPVAAMPLAGLIMRLSADDMLNIPVIGAA
                     GNAVFGNLDILFAIGVAIGFVKNKDKGIPALTGFLAIATLKKGLEIMDPEVNMGIFGG
                     IISGLVAAWTYNRFKEQKLPVVFSYFAGEKFPLTMVMILQTIAAVFLGFIWPLIQIGI
                     DNFAKLLVDMGALGVGIFMFLNRLLIPFGLHHVLNTYIYYDLGSYTSPSKEVFRGEMT
                     RFINGDPQAGLFLSGFFVVMMFGIPAICLAIYKAAYKENKEMVKGIMGSGAGTSFIAN
                     ITEPVEFSFMFLSPMLYVIHALFAGLAGIVCYLFNIRIGFTFGACIVDYLINFRIATN
                     AILILPIGIFFFALYYVTFYYLINKRNIQTLGREAKAEFGNEVTLEETELGLASKNYE
                     YMATKMLQAFGGKANILDVYSCNTRLRVEVVDPTMVEEQRIKQLGISGIIKPTEKNYQ
                     IIIGLEVTYVMAEFNKLLEE"
     gene            3421..4746
                     /locus_tag="IXT20_002581"
     CDS             3421..4746
                     /locus_tag="IXT20_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005227470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose-6'-phosphate glucosidase"
                     /protein_id="HAQ4860991.1"
                     /translation="MKKQIITIAGGGSTYTPGIVQAILNNEKRLPLSEIRLYDIDEER
                     NMDMYLIVKFMLKRKGFSNIRIRATDDPKLAFTGCDFVFSQIRVGGLEMREKDEKIPL
                     KHGLVGQETCGLGGFAYGMRSIKGLLEIVDHIQDYAPKAWILNYTNPESIVSEAVRRK
                     YPNVKMINACDMTISIEETIAVNYGYDRKNWIVTYYGLNHFGWYTSIYDKELQREIMP
                     EIIEKLITKEMQVADFNIGDKTWQKTFQMMSVITKNFPSNIPNNYLEYYLYPDMVVEH
                     TDKEYTRANMVMDGREKNTKEMADKIRRGIVEEVLNFNFGEHGQYIVDIAISLLNDDR
                     RRFMLIVPNQGAIPNLRSDAVVEIPAYVGATGVEPITLRKPISDFHKGLMEAQVAVEK
                     LLVDAYFEGSYQKALQAFTLNQTVPNARVAKKILDEMIKVNKKYWPALK"
     gene            4759..5217
                     /locus_tag="IXT20_002582"
     CDS             4759..5217
                     /locus_tag="IXT20_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HAQ4860992.1"
                     /translation="MHFFKTRNILKAVASGQIIPLTAVKDEVFSSKMIGVGYAITNHD
                     GYIHAPVKGIIDNIFPTRHAITIESESGRKILIHMGLDTVELEGSPFQIRVESNQEVK
                     EGTLLAIIDLKKLEQAQKDTVIIVVSPGIFEGNLLKQNQEVKIKDSCFEF"
     gene            5509..>6421
                     /locus_tag="IXT20_002583"
     CDS             5509..>6421
                     /locus_tag="IXT20_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ4860993.1"
                     /translation="MEKLMKWVEEKLVPPMAKIGTQRHLLAIRNGVVSTLSLILIGTF
                     FMVFINLPFPGWNEFIAPYSATIVLPFRMTMGLMAIYATFVMGSDLAKSYGLDSVTGG
                     ILSLGTFFMLQVPVNVLTPEEAPLGWVLPMSSLGASGMFAGILSMIFAVEVYRFFKNR
                     NITIKMPEQVPPAVARSFEALIPGAVILTITWLIRSVAGFDVNAALLSLFEPLTNILG
                     NNLLGVLLPMFLIHLLWSFGIHGMSIVGAVVRPMWLIMLDENAKALADGTAATQLPYI
                     APEQFYQWTVTIGGAGATIVVSVLFIFF"
BASE COUNT         2091 a          935 c         1354 g         2041 t
ORIGIN      
        1 ttatagagag ttgctatatt ttttttgttt ttatgtaatt acctccaaat caattcgata
       61 caagtattat cataaaacga gtaaaaaaga tattgataac gcttttatta tgtgttaaac
      121 tatttttgta aattatgtag ggtgctacta ttcaattagg cacgacctaa gaaagggttc
      181 tttcttaggt cgttttttgt ctttaaaaat tgtttggaga caaaaatatg tatgaaggag
      241 tctgcaagtt gttttgtttt atcatataga ggaggtgaat taacggtgta tgtgatgaaa
      301 aagctgaatc ataatgctgt attgatcgat gacgatggaa tagaaaaaat tgcgatgggg
      361 aaagggattg gcttttctgc taaagtgagt aagccatttg atgttgagga tgctgacaaa
      421 ttatttgtat tagattctaa agaaaagatc aaacgattca gtgagatagt ctcgcagatt
      481 ccaatcgaat ttatggattt tgcggaagaa gtgattgaac tgataacagc taggatctcc
      541 tctccattag attcaaatat ctatattaca ttgacagatc atatttattt tgctattcaa
      601 cgaaaaagag aagatagaga tgtcacagca attatgttgc cagaaatgag attcctttat
      661 ccaaatgaat atgatatagc agtagaagtg gtcgagcgaa tcaatcatcg atatcaaacc
      721 gagttagggt caaatgaagt aggattcatc actatgcata tcattaatgc tgagctagga
      781 gagcctaata gtttaaagag cttaaaaata gtagaaatga cgaccacgat tttgtcccat
      841 ctagaaaaat cctattcaaa cgtttatgat aaagaatcac tgacttatca tcgcttaatg
      901 gttcatatca aattcctggt taaacgcttg atttatcagg aagaaacgcc agtagaaaat
      961 attggttttt ttactgaaac atttaaacat agcaaagcgt attgtacagc actaaagatt
     1021 aaagatatca tcgaacagca atttcaactt gccattcaag aaaatgaaat catctatttg
     1081 gcaattcatc tagcaagaat ctattaaaat aagggcgtaa ctatctatag gcgcaaccaa
     1141 atgacacata gtaaatggac tatgattatt tggttgcgcc tattttagta gaaaaaagaa
     1201 ggagaatgaa atgggaaaag tgatttttaa ttcggatgat tttggctata gccatggtgt
     1261 gaattatggg attatggatg cttatcagcg aggaattttg acatcaacta cattgatggc
     1321 aaatatgccg ggtttcgagc atgctgtgaa actaagaaag gaaatgccta ggctaggtgt
     1381 aggagtccat ttaacattga cgtgtggcaa accactcctc aaaactgtcg atactcttat
     1441 cgaaagcgga gagttcagat ccttatctta ttatcagcat ccatttaaca ttgacaaaga
     1501 tcaactgtat caggaatgga atgctcagat tcaaaaaatt tatcatgcgg ggatcatacc
     1561 aactcattta gacagtcatc atcatactca tactttggat gaaaatcagg aggtagttgt
     1621 tacattagca aaaaaatatg atttgccggt tcggagcaac tttgaacgaa aagatgaagt
     1681 tcgtcatgtc gattattttg agccttgttt cgatgtcgta ggagaaactg atgaaaaaaa
     1741 acgacaaatc aatacgagtg tggaagaata tttggcaagt ttactcatga tattgagaga
     1801 agataaaacg atcgaagtca tgtgtcacgc agcgtatttg gatcaggaat tgttaaatgg
     1861 ttcaagtttt gtgtatcctc gtacccacca agtcgaattt ttaattcatt cagcatttgc
     1921 tcaaaaagtg aaaaatgatc ctttgattga gttagtaaca tatgaggata tttaatagag
     1981 gaggaataaa aatgaatgtt aaatcaaatg tgcagaagct tggaagagcg atgctacttc
     2041 cagttgcagc gatgccatta gcgggattga tcatgcgttt atcagctgac gatatgctga
     2101 acatcccagt aattggtgca gctggaaatg ccgtttttgg aaacttggat atcttatttg
     2161 ctataggagt tgcaatcggt tttgtcaaaa ataaggacaa aggaatacct gcacttactg
     2221 gttttttagc gattgctacc ttgaaaaaag gcttagaaat tatggaccca gaagtgaata
     2281 tggggatttt tggtggaatt atttccggtt tagtagcggc atggacgtat aatcgcttta
     2341 aagaacaaaa attaccagta gttttttctt attttgcggg cgaaaaattc ccattgacta
     2401 tggtgatgat tctacaaaca atcgcagctg tttttttggg ctttatctgg ccgctcatcc
     2461 aaattgggat tgataatttt gcaaaactgc tcgttgatat gggagcatta ggtgtgggga
     2521 tatttatgtt tttgaatcgt ttgctgatcc cctttggact gcaccatgtg ttgaatacgt
     2581 atatttatta cgatttagga agctatacat ctcctagcaa ggaagttttc agaggagaga
     2641 tgacacgctt tatcaacgga gatcctcagg ctggactgtt tttatcaggt ttttttgtag
     2701 taatgatgtt tggtatacca gctatatgtc tagctatcta taaagcagct tataaagaaa
     2761 ataaagagat ggtcaaagga atcatgggaa gcggtgcagg aacttcattt attgcaaata
     2821 tcactgagcc tgttgaattt agtttcatgt ttctttctcc tatgttgtac gtgatccatg
     2881 cactttttgc cggtttagca gggattgtct gttacttatt caatatacga attggtttta
     2941 cattcggagc atgtattgtt gattatctca tcaattttcg aatcgctacc aatgctattt
     3001 tgattcttcc aatcggaatt ttcttttttg ccttgtatta cgttactttt tattatttga
     3061 tcaataagag aaacattcag acattaggac gtgaagctaa ggcagagttt ggcaatgaag
     3121 taaccttaga agaaacagag ttagggttag caagtaaaaa ttatgaatat atggcaacaa
     3181 aaatgctaca agcgtttgga ggaaaagcca atatcctgga tgtctatagt tgtaatacac
     3241 gcctacgggt agaggtagtg gatcccacca tggtcgagga acagcgaatc aagcaattag
     3301 ggataagcgg gatcattaaa ccaactgaaa aaaattatca aattatcatc ggcttagaag
     3361 tgacttatgt tatggcagaa ttcaataaat tattagaaga atagaaagga aaagatgatg
     3421 atgaaaaaac agattatcac tatagctgga ggcgggagta cgtatacacc aggaatcgtt
     3481 caagccattt tgaacaatga aaaacggttg ccattatctg aaatacgttt atatgatatt
     3541 gatgaagaaa gaaatatgga tatgtacttg attgttaaat ttatgctgaa aagaaaaggt
     3601 ttttcaaata ttcgcattcg tgcaacggat gatccaaagc ttgcttttac aggatgcgat
     3661 tttgttttct cacaaattcg tgttggagga ttggagatgc gagaaaagga cgaaaagatt
     3721 ccacttaaac atggattagt aggacaagaa acgtgtggac tcggaggatt tgcttatggg
     3781 atgcgctcaa tcaaaggttt attggaaatt gtcgatcata tacaagacta tgcccccaaa
     3841 gcatggattt tgaattatac aaatccagag tcgatcgttt ctgaagctgt tcgtcgtaaa
     3901 tatccgaatg taaaaatgat taatgcctgt gatatgacta tttcaattga agaaacgatt
     3961 gcagtgaatt acggttatga tcgtaagaat tggatcgtta cgtattatgg tttaaaccac
     4021 tttggctggt atacatcgat ttatgataaa gaattgcaac gagaaattat gccagagatt
     4081 attgaaaaac taataacaaa agaaatgcaa gtcgccgatt ttaatatagg agataaaact
     4141 tggcaaaaaa cctttcaaat gatgtcagtc atcacgaaaa attttccaag taatatcccg
     4201 aataattact tagagtatta cttatatcct gatatggtcg ttgagcatac tgacaaagag
     4261 tatacacgtg ctaatatggt catggatgga cgagagaaaa ataccaaaga aatggctgat
     4321 aaaatacgcc gcggtattgt ggaagaagta ttgaacttta attttgggga gcatggacaa
     4381 tatatcgtcg atatagctat ttccttgttg aatgatgatc gtcgtcgctt tatgctaatc
     4441 gttcctaatc aaggggcgat tccgaatcta cggtcagatg cagttgttga aataccggca
     4501 tatgttggtg caactggtgt ggagccaatt acactaagaa aaccaatcag tgattttcat
     4561 aaaggattga tggaggcaca agttgcagta gagaagttac tagtagatgc ttactttgaa
     4621 ggttcttatc aaaaagcttt acaagcattt acattgaatc aaaccgtacc aaatgccaga
     4681 gtcgcaaaaa aaatactaga tgaaatgatc aaagtaaaca aaaaatattg gccagcatta
     4741 aagtagaggt gataaaaagt gcatttcttt aaaacaagaa acattctaaa agctgtagct
     4801 agtggtcaaa ttattccatt aacagcagta aaagacgagg tcttttcttc aaaaatgata
     4861 ggagttggat acgctataac gaatcatgat ggatatatcc atgcgccagt taaaggaata
     4921 atcgataata tatttcctac ccgacatgcc ataacgatcg aaagtgaatc tggcagaaaa
     4981 atactgatcc atatgggttt agatacagtt gaactcgaag gaagtccgtt tcagatacgg
     5041 gttgaaagta accaggaggt aaaagaaggg acactgttag ctattataga tttgaagaag
     5101 ttggagcaag cacaaaaaga tacggtgatc attgtagttt ctccaggcat ttttgaagga
     5161 aatttattaa aacaaaacca agaagtaaag attaaagata gttgttttga attttagtgt
     5221 acgatgaaaa aagttaaata gggtgatatt tatgggtgaa tttacaaaat ttttttgaaa
     5281 tgatatgttt caatcactag atttaatcat tttgattttt gttaattatc gaattttttt
     5341 gttgctccta tgatttagaa aacttatttt caggattctt aaaaataaat ttgattattt
     5401 aagagaatat attgaaataa aatttcttaa ttatataatt agttatgaac attctttgcg
     5461 ttgtcatgca atgaatggtt catattttat tgaaaaggag tggttttagt ggaaaagctg
     5521 atgaaatggg tcgaagagaa acttgtccca ccaatggcaa aaattggtac acagcgtcat
     5581 ttattagcaa ttcgtaatgg ggttgtttca acattgtcat tgattttgat tgggacattt
     5641 tttatggtct ttatcaattt accgtttcct ggatggaatg aatttattgc cccttattcg
     5701 gcaacgattg ttttaccctt taggatgacg atgggattga tggctattta tgcaactttt
     5761 gttatgggtt cggatctagc taaatcatat ggattagata gtgtgacagg tggtatatta
     5821 tctttaggaa cgttctttat gttacaagtt cctgtaaacg ttttaactcc tgaagaggca
     5881 cctcttggct gggtcttacc tatgagttct ttgggagcat ctgggatgtt tgcggggatc
     5941 ttatctatga tttttgcagt ggaagtatat cgtttcttta aaaatcgaaa tattactatc
     6001 aaaatgccag aacaagtgcc acctgctgtt gcccgtagtt ttgaggcatt gattccaggt
     6061 gcagtcattt taacaattac ttggctgatt cgaagcgtcg cgggctttga tgtaaacgct
     6121 gccttattat cgttattcga gcctttaacc aatattttag ggaataattt gcttggtgtt
     6181 ttactaccaa tgtttttgat ccatttgttg tggtcatttg gtatccatgg catgagtatt
     6241 gtaggggctg ttgttcgtcc aatgtggctg atcatgttgg atgagaatgc aaaggcattg
     6301 gcagatggta cagctgccac ccaattaccc tatatcgctc ctgaacagtt ttatcaatgg
     6361 acggtaacaa ttggtggtgc aggagcaacg atcgtggtat ctgttttgtt tatattcttc
     6421 t
//
LOCUS       DACGLE010000107         6173 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0534
            SAMEA3304449-rid9560453.denovo.113, whole genome shotgun sequence.
ACCESSION   DACGLE010000107 DACGLE010000000
VERSION     DACGLE010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304449
            Sequence Read Archive: ERR985064
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6173)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6173)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702225.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702225.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 20:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,045
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 27 of 67
            Pseudo Genes (incomplete)         :: 25 of 67
            Pseudo Genes (internal stop)      :: 19 of 67
            Pseudo Genes (multiple problems)  :: 4 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6173
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304449-rid9560453.denovo.113"
                     /strain="VREN0534"
                     /db_xref="taxon:1352"
     gene            725..811
                     /locus_tag="IXT20_002584"
     CDS             725..811
                     /locus_tag="IXT20_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011302749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HAQ4860994.1"
                     /translation="MFELFICPLLIEVCVSLFSYWLNNRNKK"
     gene            complement(932..1621)
                     /locus_tag="IXT20_002585"
     CDS             complement(932..1621)
                     /locus_tag="IXT20_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4860995.1"
                     /translation="MAFYLPYLLIFASILGIIWLSYKAYQTRYSLKGSKIRFKRFLLP
                     GVIFTLSIVLSGLFGVLEGNKRISRSILLGDITQKYEAACDKKKKEQVLTQKMEEFTT
                     CYEEMNDIFVNQEKRLTDKNMEKLTRLYQNLPEKQQKEVQDNYERTKKDVQYFKDTQT
                     EEACYNLFSDTIPFSTSEQERKERQQSVTYERYKALLQQATNIQNPTKKETALNYLKS
                     VKEWLDQQQQN"
     gene            complement(1866..2780)
                     /locus_tag="IXT20_002586"
     CDS             complement(1866..2780)
                     /locus_tag="IXT20_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HAQ4860996.1"
                     /translation="MATVKVSRTTSCSRAINYAEPRATVKTGINCDIDYAKSEMKQIR
                     MLYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPDHQIAIYTHTDKDHIHN
                     HIVINSVNLETGNKFQAHGQAFLNKCYDLNDEICLEHDLTITERGKKPEKRTMSEIKL
                     KEKNEPVWKDEIRFAIDQTMKNKKTRTYNQFCDSLKTFGIHCLNRGKNFTYELIHKKK
                     KVRSNKLGKDYEKETILHELDRRERAYNQQRTNELRRARASIRETAKEVRREQRTLTT
                     EQPEIRPTRRNHDQHVERGADYQQELSF"
     gene            complement(2762..3142)
                     /locus_tag="IXT20_002587"
     CDS             complement(2762..3142)
                     /locus_tag="IXT20_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HAQ4860997.1"
                     /translation="MSEQNQYLASNHSKKYTYRSEPKQISFRVSESEFAKLKQSAEAL
                     QMSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVDPG
                     DAQRLSENLKIMQKELQKIWQQLK"
     gene            complement(3592..3879)
                     /locus_tag="IXT20_002588"
     CDS             complement(3592..3879)
                     /locus_tag="IXT20_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HAQ4860998.1"
                     /translation="MDFTKEEKLLNAISKVYNEATIDDYPDLKEKLFLYSKEISEGKS
                     VGEVSMKLSSFLGRYILKHKFGLPKSLIELQEIVSKESQVYRGWASIGIWS"
     gene            complement(3899..4123)
                     /locus_tag="IXT20_002589"
     CDS             complement(3899..4123)
                     /locus_tag="IXT20_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin hiracin-JM79"
                     /protein_id="HAQ4860999.1"
                     /translation="MKKKVLKHCVILGILGTCLAGIGTGIKVDAATYYGNGLYCNKEK
                     CWVDWNQAKGEIGKIIVNGWVNHGPWAPRR"
     gene            complement(4763..5296)
                     /locus_tag="IXT20_002590"
     CDS             complement(4763..5296)
                     /locus_tag="IXT20_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HAQ4861000.1"
                     /translation="MQPQETFTIKELSDLFKMSRQAISKHIQKLDSSMIAKNERGYKV
                     VLRSGVLQLARNLDNQRLLDKLHKHEQNNDKNAYIPNKNVVANKLLDQLREENEFLRE
                     QLALKEQNNSHIQKLLDQQQQLTLQANQQIEKLQEQLQLTYTEESSENSSTTLSEATE
                     TMDEQTKNWKKWWQLWK"
     gene            complement(5317..6159)
                     /locus_tag="IXT20_002591"
     CDS             complement(5317..6159)
                     /locus_tag="IXT20_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HAQ4861001.1"
                     /translation="MREKGDKTVRFTFDQLKDLSNYKATANVRFVEDLKATYEKILSL
                     RFGRRSESGLSYDMFVMFTEFKINGDVENPYVDIRIYEKALPLLNNLDEWVRYSLQQF
                     NELQSGYAKTMFRLLKQYRTKGFAYFSKEDFLELLDIPKSYKQPDIDKRVIKPIRQEL
                     TAIFKGLTIKKKYGKGRGKPVIGYQFTFKPEIKKADDFNKHAPTKEDQNKALALADKQ
                     KEYEEPATTINEKIKQKKRELELLQQIKQLEKEKKALEKQTESALSEKEKKSLLSDLD
                     NLFQ"
BASE COUNT         1633 a         1151 c         1042 g         2347 t
ORIGIN      
        1 aaaaaaacaa attcatttca accggtgtaa attttcttaa aggaattgta tttaattcat
       61 ggtggtattt tacaatctca ttagccatat aatcacctca atccaattat gaatgacatt
      121 ttttaaatgt caagacattc atatttgacg ttcaaacaga caaaatgtcc ttcataaaca
      181 gacaaaatgt ccttcataaa cagacaaaat gtccttcata aacagacaaa atgtccttca
      241 taacattctg tatccattgg tagagtaagg acacagctta cggtaaagag gtttaaaaag
      301 aggtttaaaa agagatataa aaagaggggg gctaattttc ccgcctaaaa accacttctt
      361 ttttctttaa acaggttcgc gcatttttca aaactgccaa tccaaaacct tgcttcctac
      421 ggtcgccaaa aaggtgtgat cgctacgctc tcacattgta ctgcgcagtc gcctaaaggc
      481 tcctttgcta aaaacatgtt ggtaaagagg cctttgagcg gttctgaatg cttatctaag
      541 ccgttttaag acagtcttgg ataaaaatcc ttatctagct ttaaagcgcc ttacaggcgg
      601 tcagggcaag ccctaacaac cctaaaataa gcgatactta aaaacaccac acaagtttaa
      661 ttttttaaga tataataact tgtcgcctat ccgacagtct tgcgactgga aggaggtgta
      721 tctaatgttt gaattgttca tttgccccct tcttatagaa gtttgtgtct ctttattcag
      781 ttattggttg aataaccgaa acaagaagta aaatcaataa aggcgacatc aacctgaaaa
      841 aaagccccag agaatccgac ctctctgggg ctttcgtgta tctaatgttt gatcatttgc
      901 atgtttagtt taacatgatt gccgatcaaa ttcaattttg ctgttgctga tctaaccatt
      961 ccttgacact ttttagataa tttaatgccg tttctttttt cgttggattt tgtatgttcg
     1021 ttgcttgctg aagcagtgct ttataacgtt cgtaggtaac tgattgttgc cgttcttttc
     1081 gttcctgttc actagttgaa aaagggattg tatcactgaa taaattataa caagcttctt
     1141 ctgtctgcgt atctttaaaa tattgcacgt cttttttggt tcgctcatag ttgtcttgaa
     1201 cctctttttg ctgtttttct ggcaagtttt gataaagacg agtgagtttt tccatgtttt
     1261 tatccgtcaa acgtttttct tgattcacaa aaatatcgtt catttcctca taacaagtgg
     1321 taaactcttc cattttttgt gttaacactt gctccttttt ctttttatca catgctgctt
     1381 catacttttg agtgatatcc cctaataaaa tacttcttga aatacgttta tttccctcta
     1441 aaacgccgaa taaaccactt aaaacgattg ataatgtaaa tataacccct ggcaataaaa
     1501 acctcttaaa tcgaatttta gagcctttta atgaatatct tgtttgataa gccttatagc
     1561 taagccaaat aattcctaaa atactggcaa aaatgagtag atagggtaaa tagaatgcca
     1621 tttctattta ttctccccat accagccaaa aggctaacag tgtcatgatt aagcaacata
     1681 agggggcaag aatatccatt gatcgctgca taattacttt tttctgttct ttatttcgct
     1741 ggtttaatag cgcatggtgg aattttttga cttgtgaatc aattgtagtt tgtattcttt
     1801 ctttggtata aggtgcaaga aaagcacgtt gtttctttat ttcattttct atgtcttgta
     1861 acgttttaga acgataattc ttgttggtag tctgctcctc tttcaacgtg ttggtcatgg
     1921 tttcttcgag tcggtctaat ttccggttgt tcagtcgtaa gagttcgttg ttctcttcgt
     1981 acttcttttg cagtttcgcg aatgctagct cgagctcttc gtagctcgtt ggttcgttgt
     2041 tgattgtagg ctcgttctcg tctgtccagc tcatgtaaaa tcgtctcctt ttcgtaatcc
     2101 tttcctagtt tgttggatcg tacttttttc tttttgtgga tcaattcgta agtaaaattt
     2161 ttcccacgat tcaagcaatg aatgccgaat gttttaagag aatcacaaaa ttggttatac
     2221 gttcgagttt ttttgttttt cattgtttga tcaatcgcaa accgaatttc gtctttccaa
     2281 acaggttcat ttttttcttt taatttgatt tcagacatgg ttcgtttttc gggtttcttc
     2341 cctcgttcag tgattgttaa gtcatgttca agacaaatct cgtcatttag gtcataacat
     2401 ttgtttaaaa atgcttgacc atgggcttga aatttgttcc cagtttccag attgacggag
     2461 ttaatgacaa tgtggttatg aatatggtct ttgtctgtat gtgtgtaaat cgcgatttgg
     2521 tggtcagggg ctattttttt cgcatactct gcccctagtt gattggcttt ttcagcggta
     2581 atttctcctg gtcgaaaaga ctggatcaat aagtgtgctt gtacacgatc gtcttttcca
     2641 tacagcatgc gaatttgttt catttcgctt ttggcataat caatgtcaca gtttatccct
     2701 gttttgacgg ttgctcgtgg ttcggcgtaa ttgatcgctc ggctacaaga agtggttcgg
     2761 cttactttaa ctgttgccat atcttttgta gctccttttg catgattttt aggttttcgg
     2821 ataagcgttg ggcgtcgcca ggatcaacgt cgtgttggtg tgtgttgcac cacttggcaa
     2881 tttgattgat attcccgcca gctttggcta aatcgcgcgc gattgcctga gcaatgtctg
     2941 gcgccacttt cggtgtcacc agacgcgcgt tttgtgcctt ggctttcacg aaagccggca
     3001 cactcatttg caaagcttca gctgactgct tcagctttgc aaattcggat tcgctcactc
     3061 gaaaactgat ttgttttggc tcggatcgat aggtatattt tttagaatga ttgctagcca
     3121 aatattgatt ttgttcgctc ataagtccca ccctttcagt acgtcctacg gacgtatcgt
     3181 tcgcaatacc attgtaccta aaaaggtaca ataagtaaag ctcactaaag ctcactaaag
     3241 ggcttggcaa gctctatatt tgctagccaa atacctacgt atttggtgca aataaccgct
     3301 tgtcaaaggg gcgcaagctc ccccttgaat ccccctcccc cccactctcc acaatacaaa
     3361 aaagactagc aaatgctagc cactttttgt aaagtttcgc ttgtttcacc ttcggtgaaa
     3421 ttgttgcaaa tcaataatta ggtagagcta catttttttc gttgttcaaa gattcttatg
     3481 ccatttacta ttaataaatc tataaatatg ataatttttc ggctctataa tttatgggac
     3541 tgatgaatca gaattgattc ttcagtccca ttttcatttt attccaaaga attaactcca
     3601 aataccaata gaagcccatc ctctatatac ttgagattcc ttactaacaa tttcttgtaa
     3661 ttctattaaa gatttaggta atccaaattt atgttttaaa atatatcttc caagaaaact
     3721 acttaatttc atactaactt caccaacact ttttccctca ctgatttctt tagaataaag
     3781 aaagagcttt tcttttaagt caggatagtc atctatagtt gcttcattgt atactttact
     3841 aattgcattt aaaagttttt cttctttagt aaaatccatt ataataacct ccctaccact
     3901 accttctagg tgcccatgga ccatgattaa cccaaccatt aacaataatt tttccaattt
     3961 ctcctttagc ttgattccaa tctacccaac atttttcttt gttacaataa agaccatttc
     4021 catagtaagt agctgcatca acttttattc ctgtaccgat gccagctaga caagttccta
     4081 atattcctag aataacacaa tgttttaata ctttcttttt catgattgtt tttccttttc
     4141 gtttttttaa aattgcttgt tatattaaat aatcttagta attttattat ataacatttt
     4201 attatataac aatgattaac gtcataaaaa gtagttttga attcataatc gtttaaataa
     4261 aattataaga tcagttattt aaacagctag tagacataat gtgtatacta ccaagtaacg
     4321 cctgtaccaa gggatccagg cgctgatttt gtgaattttt attggtctag gttaaaagct
     4381 ctttttaagg cttggttttc tgcggttgtt tctttctaat tctttgtgaa tattgatcgg
     4441 tcatttttta aatacttgtt atttttgttt gtgtttgtta tgctttttac atatctcata
     4501 ttcatttatt attcttttct aaggttaata ataggataat ctctttcaga aagactgctc
     4561 gtacgagcag tcttttctta tttatcaagt ttttctacaa atacttcctc attttgactg
     4621 ttgtagtaat taggattgag ttttagttcc ccttttttat ttcctttgtt ggccactgat
     4681 aataacattg ggctaaagct taatattcct agtaggatag aaccgaatag caagtgtttt
     4741 tttgtcataa gtatttcctt cttcatttcc atagctgcca ccattttttc caattctttg
     4801 tttgttcgtc cattgtttct gttgcttcag aaagagtcgt tgaactattc tctgaagact
     4861 cttccgtata agtcaactgc agttgctcct gaagtttttc gatttgttgg ttggcttgca
     4921 gggtgagttg ttgttgctgg tcgagtagtt tttgtatatg agaattattt tgctctttga
     4981 gggcaagttg ttctcgtaaa aattcatttt cttcacgcaa ctgatctagc aacttgtttg
     5041 caaccacgtt tttgtttggt atataagcat ttttatcgtt gttttgttca tgtttatgca
     5101 acttatctag caacctttga ttgtctaagt tgcgagcaag ttgcaatact ccagaacgca
     5161 atacaacctt ataaccacgc tcatttttag ctatcatgga agaatctaat ttttggatat
     5221 gtttcgatat tgcttgccta ctcatcttaa aaagatctga caattccttt attgtaaagg
     5281 tttcttgtgg ttgcatactg gttgcctcct aggttgctac tggaataaat tatctaaatc
     5341 agataataaa ctttttttct ctttttccga aagtgcggac tctgtttgtt tttcaagagc
     5401 ttttttctct ttttctagtt gtttgatttg ttggaggagt tcaagttctc gtttcttctg
     5461 tttgattttt tcgttaattg ttgtagcggg ttcttcgtat tctttttgtt tatcagctaa
     5521 agccaatgct ttattttggt cttctttggt tggtgcatgt ttgttaaagt cgtctgcttt
     5581 tttgatctca ggtttgaacg taaattgata gccaataaca ggttttcccc gtccttttcc
     5641 gtatttcttt ttgattgtta gtcctttgaa gattgcagtt agttcttgac gtataggttt
     5701 tataactcgt ttatctatat caggttgctt ataactttta ggaatatcta ataattcaag
     5761 aaagtcttct ttggagaaat atgcgaaacc tttggttctg tattgcttta acagacgaaa
     5821 catagtcttt gcatagccac tttgtagctc gttgaattgt tgtaaagaat agcgtaccca
     5881 ctcgtctaaa ttatttaaca aaggcagtgc tttttcgtaa attcgtatat ctacgtaagg
     5941 attttctacg tccccgttga ttttaaattc tgtaaacatt acaaacatat cgtaagaaag
     6001 cccagactca cttctacgac caaagcgtaa agataagatt ttttcataag ttgcttttaa
     6061 atcttctaca aatcgtacat ttgcagttgc tttgtagtta cttaaatctt ttagttggtc
     6121 aaacgtaaaa cgaactgtct tatccccttt ttcacgcatt ctagacacga tgg
//
LOCUS       DACGLE010000108         6152 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0534
            SAMEA3304449-rid9560453.denovo.114, whole genome shotgun sequence.
ACCESSION   DACGLE010000108 DACGLE010000000
VERSION     DACGLE010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304449
            Sequence Read Archive: ERR985064
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6152)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6152)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702225.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702225.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 20:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,045
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 27 of 67
            Pseudo Genes (incomplete)         :: 25 of 67
            Pseudo Genes (internal stop)      :: 19 of 67
            Pseudo Genes (multiple problems)  :: 4 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6152
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304449-rid9560453.denovo.114"
                     /strain="VREN0534"
                     /db_xref="taxon:1352"
     gene            1323..2363
                     /locus_tag="IXT20_002592"
     CDS             1323..2363
                     /locus_tag="IXT20_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein RepA"
                     /protein_id="HAQ4861002.1"
                     /translation="MTDFNFFKADRVYNELFYQFPKVFIVSDEYKKMKDSTKIAYMLL
                     KARLEIAISKRQIDEEGNVYFTYTTNELCSVLNCQKQKAIAIKKELESFGLLLQKQMG
                     FNKQLGKNNPNRLYLAELKVSENDIYLLEKFDRENRENVDKSEGMKIIPTLDEKSDAE
                     SLGAQEGMKIIPCQNVDKSEGMKIIPELNNNILDTNRHNIDTEKDRLQDQLLLDNFET
                     IMTNDSIATFVPERVLNLIKTFSSSYSEAQKTVQTIHNAKKKAEIESGISIVFEELDS
                     YYVNAEQELYTTLLKAYQKLKTEKVENIQNLIFVYVKNWFIEKPIAAKVSSEKRLNYE
                     SSPSTITKDWLE"
     gene            3188..3520
                     /locus_tag="IXT20_002593"
     CDS             3188..3520
                     /locus_tag="IXT20_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraE"
                     /protein_id="HAQ4861003.1"
                     /translation="MSYTGILSLKDICHYGKRCTATEKITKKLSTGQSKTVVQCKKYI
                     IQKDKVSEEMIYYIGKQKQIILKDPIPLKELYPTIKHVYDQNGVLIGRRKNGVLRCTA
                     KGMGRLIS"
     gene            4139..4294
                     /locus_tag="IXT20_002594"
     CDS             4139..4294
                     /locus_tag="IXT20_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 domain-containing protein"
                     /protein_id="HAQ4861004.1"
                     /translation="MKNYDPNIRLGTHTIKVSFQRWDYKGFVTFRRGGNCKGLDVLAL
                     DEDDLYD"
     gene            4298..4480
                     /locus_tag="IXT20_002595"
     CDS             4298..4480
                     /locus_tag="IXT20_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 domain-containing protein"
                     /protein_id="HAQ4861005.1"
                     /translation="MTDNPIGFGLLPEDDEGDEWFKMTLTNDKGDELSVEDTWSYLSD
                     YIVSVEIIDFVADKEE"
     gene            4647..4886
                     /locus_tag="IXT20_002596"
     CDS             4647..4886
                     /locus_tag="IXT20_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4861006.1"
                     /translation="MARPVLDAMDMDTLTKGRYVQLLARSYSPKDVFKALSKGTDMEK
                     ERLRKEFDYWRIYNGIKDLKPARPVVKRKKKITQE"
     gene            4996..5190
                     /locus_tag="IXT20_002597"
     CDS             4996..5190
                     /locus_tag="IXT20_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4861007.1"
                     /translation="MEEKLLKTMKQKHLKRLSVMQYISDMQITGKEKACLLGSMKNFE
                     QLRRTYVKIRSNCQLLLEVS"
     gene            5317..6006
                     /locus_tag="IXT20_002598"
     CDS             5317..6006
                     /locus_tag="IXT20_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sortase"
                     /protein_id="HAQ4861008.1"
                     /translation="MNKNNKTLFNPYVGVIIVFLLFSYFAYQGHQKDKIAEKVDGVTK
                     TIHIQEQDKTYQDNLTSLTDISKRQLITNQKQALEKDLKGEIAGKLVFSSTGFILPVF
                     QGANSDTLSLGVASTYYPDAEMGNGNYILAGYNVDTPSVLLSDMIDLSEGEEIDLIDA
                     HYKYQYEVSKRGKIYDYSQVVQSKENNSFLSLPKAGKKPLLTLLDFEANNMKKCDVIQ
                     CELVNVEKINK"
BASE COUNT         2294 a          813 c         1074 g         1971 t
ORIGIN      
        1 atgaaatacc tctttgttac tcataattat accataatta gtcacgaatg caaccaatag
       61 aaaaaaatac ttagaacggt agatcatcgc taataacaat gcattgtcca ccaaatggat
      121 tttgtgaagc attaaataca ttgttattaa ttgtcgctgc ttttaactag gcctctcccc
      181 ttctattata aaagaagata tatattattt cttaattacc tactacctta aaccaaaatt
      241 taagtactac ctaatttaag tagtacttaa ataatctaaa aaatagttat cccaattgtt
      301 atgcttttat aatacgatta tttgtaacaa aatgaatatt atataattaa acactaccat
      361 atttaggtag tgtttaatta ttagcaaaac aaaaaatatt tattctattc tcttaacttc
      421 aactaagtta tgctgcacac ctttatataa atcatatgca actcattagc tagacaaact
      481 acaagcatgt agttttatct gttaattatg tactacctaa ataaattagt ttataattaa
      541 caagtatata tatttaaaaa tttaagatta atttttttat gacttataca aatttatctg
      601 atattctctt attaagaact acctaatttg ggtagttctt attttttaca ttttaagtag
      661 tatttaattg caatttggta gtattttttt attatcttat tcctagaacg gtagtactta
      721 atataaatat aggtagtatc taatttaagt attaatggta gtatctaatt taacaggtag
      781 tatgtaacta ctttaaagta attattttca gattaacaca gtagtatctt attaaataag
      841 ttaattttaa tggtagtaca taattattat atatatctga cattggtagt acttattgta
      901 ttttttagta gtatataatc tattgttttc tagcctaaag ccttgtctta taagaataat
      961 ataatatata ctactgaaat aaggtagtat atatcactag taatggtagt atgttttata
     1021 tgcataaggt agttcttaat ttattttttg ttttgacact ggtagtactt aattatcttt
     1081 aattttaaaa ctaaatttta ttcatacgtt cttctggtag tatttaatta taaataatat
     1141 ttatttaaag tagttcttaa tgactttaaa ggtagtacat aattcatttt atttctgatt
     1201 ttaggtagta tttaattaaa aaacagtttt aatgcgtttg cattttttat ttatctttgc
     1261 tattttcgaa aagagtaggt aattttacat aaataaaaca acgtgaaagt agaggatcta
     1321 caatgactga ttttaatttt tttaaggcgg atagagttta taacgaatta ttttatcaat
     1381 ttccaaaagt ctttattgtt tctgacgaat acaaaaaaat gaaagattca actaagattg
     1441 cctatatgct tttaaaagca agattagaga tcgcaatcag caaacggcaa atcgatgaag
     1501 aaggtaatgt ttattttact tatacgacaa atgaactatg tagcgtatta aactgccaaa
     1561 aacaaaaagc gatagcaatc aaaaaagagt tggaatcctt tggtttatta ttacaaaagc
     1621 agatgggatt taacaaacag ttagggaaaa ataatcctaa tagactatat ctagcagaat
     1681 taaaagtctc agaaaatgat atctacttac tcgaaaaatt tgatagagag aatagggaaa
     1741 acgttgataa atcagagggt atgaaaatca tacccaccct cgacgaaaaa tcagacgctg
     1801 aatcccttgg ggctcaagag ggtatgaaaa tcataccgtg ccaaaacgtt gataaatcag
     1861 agggtatgaa aatcatacca gaacttaata ataatatatt agacactaat agacacaata
     1921 tagacactga aaaagaccgc ctacaagatc aattgttgtt agacaatttt gagacaatta
     1981 tgacaaacga cagcattgct acgtttgtcc ctgaacgagt attaaatttg ataaaaacat
     2041 tttcttcaag ttacagtgaa gctcaaaaaa ccgtccagac tattcataat gcaaagaaaa
     2101 aagctgaaat agaaagtggt atttcgatag tttttgaaga actcgatagt tattatgtca
     2161 atgcagaaca agaattatac acgacactgt taaaagccta tcaaaaatta aaaaccgaaa
     2221 aagtcgaaaa tatccagaac ctgatttttg tctatgtaaa aaattggttt atcgaaaaac
     2281 caatagctgc taaagtatca agtgaaaaac gtttgaatta tgaaagctcc ccaagcacta
     2341 ttacgaaaga ctggttagag tgaaacttta ctgacaaagt gattgttgct tcatggagta
     2401 acattaaaat taaaaatttt ttattattct aattatatta cgaatattac aaaataatag
     2461 ctattttaat agaatttata agttaaaata ttatgataaa aatcacaaac gtagcctttt
     2521 acgtcataag tgttaaaaga aataattaat ttcaaatata gaaaagggaa cgtatgtttg
     2581 catactcctt ttttttgagt tataataata cgcgaagaga gataatttca ttatttaaac
     2641 aagctaaaaa tcttgatatt acttcttttc aggcaatacg aatattattc gcttacattt
     2701 ttttgtataa atttaattgt ctgctatgcc tgtgatgaat tgattttcgt tctgcacttt
     2761 tggacgataa atttggacaa ttgaaaatac aaaaaaaaga aaggcggtat gaactcggaa
     2821 ggctggtaat cttcgtcagc gttcatacac ggcattaaga tttggccctt aatgcgttaa
     2881 cctttcagtg ttacggtatt acccgcaacg gatttagtat aacagatatt caaaagtgat
     2941 accacttttt ttggctgtgt taaggttaaa aatacgtgat attacgatga atctatgcct
     3001 aagcataatg ttataaaaat gtccgtgtgg ctctttgccg tatgacgaac gctgaaatta
     3061 gagggaacgc atgtttggat gcgttccctc ttttttgttt gtcatgaacg aaaatcaatt
     3121 ctctattttt gaaaaataac aagaaaaact tgttaaaaat atttaaagaa attgggtggt
     3181 ttattttatg tcatatacag gaattttgag tctcaaagat atttgtcact acggtaaacg
     3241 gtgtaccgct acagagaaaa tcacgaagaa actatcaact ggccaaagta aaaccgttgt
     3301 gcagtgtaag aaatatataa tccagaaaga caaagtctca gaggaaatga tttattacat
     3361 tgggaaacaa aagcaaataa ttttaaaaga tcctatcccc ttaaaagagc tatatccaac
     3421 aatcaaacat gtttatgacc aaaacggtgt actgattggt cgaagaaaaa atggagtctt
     3481 gcgttgtacc gcaaaaggga tggggcgttt gatcagctga attgatcatt ctcataactt
     3541 gtattttatc gtttttcgct tgcttgaaaa tattgtcgag acttctaaag gaacttagag
     3601 tcactagaaa aataaggagt agacttgatc tatcaacaat tcccttcggg gagtgacggg
     3661 gtaggtttta acgattggta acgtaaaaaa ttggtcaatc gagtaaatgc gcttgtttgg
     3721 atagtggtga aatgaatttg tgtaaataat aagcgcagag taaacaagag ggaatttaga
     3781 agttagacaa gttagaatag cgtctagatt aatcatgagc agctactaag ggatctagac
     3841 tttaatgcaa actcatggaa taaggagtag gtcttctcgt aatggggagg acgcacaaga
     3901 aattgtgtcg tgtgctgcag tacatgtaga tacttgcgag gacgttgatt attggatcaa
     3961 tcgagcattt accgctgtct actagggttt ctcgtttcgg acgccataaa cgagtcgaaa
     4021 ttttttatgt atttttaagt aaacgaaaaa cgataaaata tgaacaagtc ctgaataaag
     4081 tgaaaaaatt gatataaaac atagagaaaa tgaaatttac gtaatggagg aatgacaaat
     4141 gaaaaattat gatccgaata tacgattggg cacacatact attaaggttt ctttccaacg
     4201 ttgggattac aaaggatttg tgacgtttag aagaggcgga aattgtaaag gattagatgt
     4261 tttagcatta gacgaggatg atttatacga ttaaacattg actgataatc caattggatt
     4321 cggcttgcta cctgaagatg atgaaggcga tgagtggttt aaaatgacgc ttacgaacga
     4381 caaaggagac gaattgtctg tagaagatac ctggtcatat ttgagtgatt atattgtcag
     4441 tgttgaaatc attgactttg tagcggataa agaagaataa aaattggagg aatagatcat
     4501 gaaatttttc gaagtaaaat caccatgcta ttatgcgtta atcgcagaaa ataatgaaga
     4561 agaatgtgca gaaattacga cgaataataa aaagctagta ttatcattag acatcgagta
     4621 aagaatttat ttaggaggca aattagatgg cgcgaccagt tttagatgcg atggatatgg
     4681 atactttgac aaaaggaagg tatgtacagc tgttagctcg tagttattca cctaaagatg
     4741 tatttaaagc cttatccaaa ggaacagata tggaaaaaga aagattacga aaagagtttg
     4801 attattggag aatctataat ggaatcaagg atttgaaacc agcaaggcca gtagttaaaa
     4861 gaaagaaaaa gataacccaa gagtgaagtg tagagcgtat gtgatcatag gcttttttat
     4921 ttttatcaaa acaaggaagg taggaaaact catgaattgt aaagaaatcg aaaatagaaa
     4981 aaagtgtcga aagaaatgga agaaaagtta ctaaaaacga tgaaacaaaa acaccttaaa
     5041 aggttatctg tcatgcaata tatcagtgac atgcagataa caggaaagga gaaagcctgt
     5101 ctattgggat caatgaaaaa ttttgaacag ttgagacgaa cttatgtgaa aataagatct
     5161 aattgccaac tattattaga agtttcttga cgattatgat atgaaaatga tcaattatga
     5221 tgtggcattg ttatttaata aaaagaatag taaaatttaa cgcggtttat cttggttaga
     5281 tgtaggtaaa ttaattcaaa tatagaaggt agatagatga ataaaaacaa taaaacttta
     5341 tttaatcctt acgtgggcgt aatcattgtt tttctccttt ttagttattt tgcttatcaa
     5401 ggtcatcaaa aggacaaaat cgctgaaaaa gtagatggtg taacaaaaac aatccatatt
     5461 caggaacagg ataaaaccta tcaagataat ttaaccagtt taacggatat ttctaagaga
     5521 caacttatta ctaatcaaaa acaggcatta gaaaaggatc tcaaaggtga aattgctgga
     5581 aaacttgttt tttcttctac aggatttatt ctccctgttt ttcaaggagc gaatagcgat
     5641 acgcttagtt taggagtagc ttctacttat tatccagatg ctgaaatggg aaatggaaat
     5701 tatattcttg ctggttataa cgtagataca ccaagcgttt tattatccga tatgattgat
     5761 ttatctgaag gagaagaaat tgatctaatc gatgctcatt acaagtatca gtatgaagtc
     5821 tccaaaagag gaaaaattta tgattacagc caggtagttc aatctaagga aaacaattcc
     5881 tttttatcct tgccaaaagc aggtaaaaaa ccactactca cactgttaga ctttgaagca
     5941 aataatatga aaaagtgcga tgtgatccag tgcgaattag tcaatgtaga aaaaatcaat
     6001 aaataaggga agtgagtcac attcaagaac ttgtagaaaa attgatcaat aaaatgagtg
     6061 ccatttttct gttactgttg gtatccattc tatatttaat atggacggtg ggggtgaact
     6121 actcaccact tagctaaacc taacggtttc tt
//
LOCUS       DACGLE010000109         6124 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0534
            SAMEA3304449-rid9560453.denovo.115, whole genome shotgun sequence.
ACCESSION   DACGLE010000109 DACGLE010000000
VERSION     DACGLE010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304449
            Sequence Read Archive: ERR985064
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6124)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6124)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702225.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702225.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 20:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,045
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 27 of 67
            Pseudo Genes (incomplete)         :: 25 of 67
            Pseudo Genes (internal stop)      :: 19 of 67
            Pseudo Genes (multiple problems)  :: 4 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6124
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304449-rid9560453.denovo.115"
                     /strain="VREN0534"
                     /db_xref="taxon:1352"
     gene            <1..189
                     /locus_tag="IXT20_002599"
     CDS             <1..189
                     /locus_tag="IXT20_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002419316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAQ4861009.1"
                     /translation="NSNGILRRNGLPKSMDFRKVNQTFISSVSNQRNHIPRKSLNYRT
                     PIEIFLSYVQEAFYSSLI"
     gene            complement(275..1369)
                     /locus_tag="IXT20_002600"
     CDS             complement(275..1369)
                     /locus_tag="IXT20_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HAQ4861010.1"
                     /translation="MGKLGISIYPERSTFEKDKAYLDLAHKYGYKRVFTSLLQINDDK
                     EKVLSEFKEVVDYANSLGMEVMVDINPALFEQLEISYDDLSFFHKMGAYGVRLDIGFT
                     GAEEAKMTRNPFGIKIEINMSSGTNYVDNIMSYSPNTDNLLGSHNFYPHRYSGLGYEH
                     FVFCSEKFRKYNLNTMAFVNSQSAEFGPWPTQDGLCTLEDHRDLEIATQVKHLILTGL
                     IDDISIGNAYASEEELKEMAEAFNADYPTLKVDTEEGITENERICLFDNLHSYRGDRS
                     EYILRSTMTRVYYKDKDFPPHNTRDMHHGDVLIDNEGYGQYKGETQIALKDMKNDGRV
                     NVVGRISDDELFLLDFLKPWSSFKLIENKK"
     gene            1558..2319
                     /locus_tag="IXT20_002601"
     CDS             1558..2319
                     /locus_tag="IXT20_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAQ4861011.1"
                     /translation="MKKNKVILSISLAAALLFAAGCSANNTTSSSSSTESSKSTSTAE
                     STTASSVDLNALELPQLTKDVAENEDLVEMVTNKGTIKIKLFPEHAPKAVENFMTHAK
                     DGYYNGLTFHRVIKDFMIQGGDPKGDGTGGESIWGEGFETEINNHLYNIRGALSMARS
                     QDPNSNGSQFFIVQNTDDMHDGLLKDDYPQAIIDAYKNGGYPSLDGHYTVFGQVIEGM
                     DVVDAIANIETDSSDKPKEEVKIDKINILQEAKQK"
     gene            complement(2434..2874)
                     /gene="fabZ"
                     /locus_tag="IXT20_002602"
     CDS             complement(2434..2874)
                     /gene="fabZ"
                     /locus_tag="IXT20_002602"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HAQ4861012.1"
                     /translation="MSYVLSATEVMELIPNRYPICYIDYVDTLEPGKKIVATKNVTIN
                     EDFFQGHFPDNPVMPGALILETLAQAGSILILKSEEFLGKRAYIGGINKAKFRQKVVP
                     GDVLKCHFEITKMKGSVGTALSAAYVDDKKVCECEFTFIVNQNA"
     gene            3094..3849
                     /gene="fabI"
                     /locus_tag="IXT20_002603"
     CDS             3094..3849
                     /gene="fabI"
                     /locus_tag="IXT20_002603"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HAQ4861013.1"
                     /translation="MSFLEGKKIVIMGVANKKSIAWGCAEAMLKQGAEIIYTYQNERM
                     KKSLVKLVGEEAFTVECDVSSDESIEKAMNAIGEYAGEIHGLVHAVAYANKEELSGNV
                     SDISRDGYQLAQDISSYSLIAVSKYAQPFLAPNSGIVSMTYLGSERAIPNYNMMGIAK
                     ASLEAAIRYLAAEFSPKGIRVNGISAGAIKTLAVTGVKDYQKLIQLSESRTPDQQGVT
                     IEEVGNACAFLISPLSSGIIGDIIYVDKGVHLS"
     gene            complement(3959..4972)
                     /locus_tag="IXT20_002604"
     CDS             complement(3959..4972)
                     /locus_tag="IXT20_002604"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HAQ4861014.1"
                     /translation="MQIDWNNLGFSYIKTPWRFIAKWKDGQWEEGELTEDNYLTIHEG
                     SPALHYGQQCFEGLKAYRRKDGKVNLFRPEQNSRRMNQSARRLLMPMVPEKMFVDAVK
                     QVVKANEDFVPPYGTGATLYLRPLLIGVGENIGVHPAPEYLFVVFCTPVGAYFKGGLK
                     PTNFIVSDYDRAAPKGTGAAKVGGNYAASLLPGTEAKELDYSDCVYLDPATHTKIEEV
                     GAANFFGITKDGKFITPQSPSILPSITKYSLLQLAEERLGLQAIEGDIFIDDLDQFAE
                     AGACGTAAVISPIGGIYHNNNLHVFYSETEVGPVTKRLYDELTGIQFGDIEAPEGWIE
                     VVE"
     gene            5137..6057
                     /locus_tag="IXT20_002605"
     CDS             5137..6057
                     /locus_tag="IXT20_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HAQ4861015.1"
                     /translation="MFSLLATAGTTDSSGETLTDQAVSKVNAFQRFWDGIDWDAILAA
                     FIQKSLYLIFLIVLFWILHRVGKYLIDKSFNQYSKKAILNESRLKTLHSLLNTIFHYT
                     LGFFFIYAILSAIGVPIGSLLAGAGIAGVAIGLGAQGFMSDVITGFFIIMEQQMDVGD
                     YIKLANLTIEGTVVSVGIRTLQLKATDGTVHFIPNRNITTISNLSRANMQVLLDIRIV
                     PEEGYDKIYEIIDRVNRTLAEKYQEELQTEPSIFGLVDLGNSNFAIRTVCYALNGKQY
                     ALKEEFLSSYVKELTASGFTIPNSPIAVGK"
BASE COUNT         1880 a         1214 c         1094 g         1936 t
ORIGIN      
        1 aattctaacg ggattctgcg tcgtaatgga ctgccgaaat caatggattt tagaaaagtg
       61 aatcagacat ttatttccag tgtcagcaat caacgtaatc atattccaag aaaatcattg
      121 aattacagaa caccaatcga aatatttttg agctatgtac aagaagcatt ttattctagc
      181 ttaatttgac aaatcataac ctataaaaaa acaaaataaa aaagcagaaa taactggcta
      241 tttctagcgg attatttctg caataactaa ttatctattt tttattttca attaatttaa
      301 agcttgacca tggtttcaaa aagtcaagga ggaataattc gtcatcggag attcttccta
      361 caacattgac acgtccgtcg tttttcatat ccttcaaggc gatttgtgtt tctcctttgt
      421 attgaccgta cccttcattg tcaatcagta catccccatg atgcatatct cgcgtattat
      481 gaggcgggaa atctttatct ttgtagtata cacgagtcat cgttgaacga aggatatatt
      541 ctgagcgatc ccctcgataa ctatgaaggt tatcaaacaa gcagattcgt tcgttttctg
      601 taataccttc ttccgtatct actttcaatg ttggataatc cgcgttaaat gcttctgcca
      661 tttctttcaa ttcttcttca cttgcgtacg catttccaat agaaatatca tcaatcaatc
      721 ctgtaaggat taaatgcttc acttgtgtcg caatttcaag atctcggtgg tcttccagcg
      781 tgcacaagcc atcttgcgta ggccaagggc caaactcagc agattgcgaa ttgacgaatg
      841 ccatcgtatt caaattatat tttctgaatt tttctgaaca aaaaacaaag tgttcgtatc
      901 ctaaaccaga gtatcgatga ggataaaaat tatgagaacc taataaatta tccgtgtttg
      961 gcgaataact catgatgttg tcaacgtaat tcgtgcccga actcatattg atctcgattt
     1021 tgataccaaa aggattgcgc gtcatttttg cttcttctgc tcctgtgaat ccgatatcta
     1081 agcggacccc gtaggcaccc atcttatgga aaaaagataa gtcatcgtaa gaaatctcca
     1141 actgttcaaa taacgctgga ttgatatcaa ccattacttc catgcctagg ctattcgcgt
     1201 aatcaactac ttctttaaat tctgaaagaa ctttttcttt atcatcattg atctgcagca
     1261 aactggtaaa aacccgttta tacccatact tatgagctaa atccaagtac gctttgtcct
     1321 tttcaaatgt tgaccgttct ggataaatcg agattcctaa ttttcccata ttaccatttc
     1381 ctcctgcatc ttttcgtttt tgtatccact tacattatag caaacacaaa gtaaatggtc
     1441 tacacctttt ctttttcttt taaaataaat gataaattcc aataatttaa gtaggtattt
     1501 tccctctatt ttgttatcat taaagataac aaaatagtaa attggaaagg aacagttatg
     1561 aagaaaaata aagttatttt aagtattagt ttagcagcag cattgctgtt tgctgctggt
     1621 tgttctgcaa acaacacgac ttcttctagt tcgtctacag aaagttcaaa aagtacatct
     1681 actgcggaaa gcacgactgc cagtagcgtc gatttgaacg cattggaatt accacaattg
     1741 acgaaagatg tcgccgaaaa tgaagatctg gtcgaaatgg tgacgaataa aggcacaatc
     1801 aaaatcaaat tatttcccga acacgcacca aaagcggttg aaaactttat gacacatgca
     1861 aaagacggtt actacaacgg actgactttc catcgcgtca tcaaagattt catgatccaa
     1921 ggtggcgatc ctaaaggcga tggcactgga ggggaaagca tttggggaga aggatttgaa
     1981 acagagatca acaaccacct ttataatata agaggtgcgt tatctatggc tcgctcacaa
     2041 gatcctaaca gtaacggcag ccagttcttt atcgttcaaa atacagatga tatgcatgat
     2101 ggtcttttaa aagacgacta tcctcaagca atcatcgacg cttataaaaa cggcggttat
     2161 ccttcactag atggccatta tactgtcttc ggccaagtga ttgaaggaat ggatgtcgtg
     2221 gatgctattg caaatatcga aacagatagt tcagacaaac caaaagaaga agtaaaaatc
     2281 gataaaatca atatactgca agaagcaaaa caaaaataaa aaaataacgt ctgaatcctg
     2341 catagatgac aacgtatgct aggattcagg cgtcttcttt ttacataaaa agcaagtaag
     2401 cttccttttg ttttgctata tcaggtttta tagtcaagca ttttggttga cgatgaacgt
     2461 gaactcgcac tcacagactt ttttatcatc tacatatgct gcagataacg ctgtaccgac
     2521 tgatcccttc attttggtga tttcaaaatg acatttcagc acatctcctg gaacaacttt
     2581 ttgacggaat ttggccttat tgatcccacc gatataagct ctttttccta gaaactcttc
     2641 tgattttagg ataagtatcg aacctgcttg tgccagtgtt tctagaatca acgcacccgg
     2701 catcactgga ttgtcaggaa agtgaccttg gaaaaaatct tcattgattg ttacattttt
     2761 tgtcgcaacg atcttttttc ctggttctaa agtatctaca taatcaatat aacaaatagg
     2821 ataacgatta ggaatcaatt ccattacttc agtagcagat aatacgtaag acataattaa
     2881 actccttctt tcaggcaatt gccattttca ttactttgac tatcaaagta tcatattcct
     2941 aaatatttag caatcttttt ttaatttaag aaaaaatagt ttgacaatca aaacattttg
     3001 actaataatt aagaaatcaa gaagttattt tagctattat tctctatttt tttacgacta
     3061 tcaaattttt atcagaaaca aaggagagtc attatgtcat ttctagaagg aaaaaaaatc
     3121 gtcattatgg gcgtcgctaa caaaaaaagc attgcttggg gttgtgctga agcaatgttg
     3181 aaacaagggg cagaaattat ctatacttac caaaatgaac gaatgaaaaa atcattagtc
     3241 aaattggtcg gcgaagaagc attcactgta gaatgtgatg tttctagcga cgaaagcatc
     3301 gaaaaagcaa tgaatgccat cggtgaatat gctggggaga tccacggcct tgtccatgca
     3361 gttgcttacg ccaacaaaga agaattgtcc ggaaatgtgt ctgatatttc cagagacggc
     3421 tatcaattag ctcaagatat cagcagctac tctttaattg ctgtatcaaa atacgcacaa
     3481 ccgtttcttg caccaaacag cgggatcgtt agcatgactt atctaggttc agaacgcgca
     3541 atcccgaatt ataacatgat gggtattgcg aaagcttctc ttgaagctgc tatccgttat
     3601 ttggcagctg agttctcgcc taaaggaatt cgggtcaatg gaatctcagc tggggcaatc
     3661 aaaacattag ctgtcacagg tgttaaagac tatcaaaaac tgatccagtt atctgaatct
     3721 cgtacgcctg atcagcaagg tgtgacgatc gaagaagtcg gtaatgcttg tgctttctta
     3781 atcagcccac tttccagcgg aatcatcggt gatattatct acgttgacaa aggtgtccat
     3841 ctttcataaa cttttgttgc attctttaaa gtaaaacata taaaaatcag gaaaaaactt
     3901 tttaaagtct cttcctgatt ttttcttttt acgttttttt caactcatta ctttgaaatt
     3961 attccacgac ttcaatccag ccttctggtg cttctatgtc tccaaattga atacctgtca
     4021 attcatcata caaccgtttt gttactggtc ctacttccgt ttcactataa aatacatgaa
     4081 gattgttatt gtgataaatc ccaccgattg gagaaataac agccgctgta ccacatgcac
     4141 cagcttctgc aaattgatct agatcatcaa tgaaaatatc tccttcaatt gcttgcagtc
     4201 ctagtcgttc ttcagctaac tgcaacaagg aatatttcgt gatacttggt aagatagagg
     4261 gcgattgcgg agtgatgaat tttccgtctt ttgtaattcc aaagaaattt gctgcaccta
     4321 cttcttcgat tttcgtatgt gtggctggat caagataaac acaatctgag taatccagtt
     4381 cttttgcttc tgttcctgga agtaaactcg ctgcgtaatt tccgccgact tttgctgcac
     4441 cagtcccctt aggagcagca cgatcgtaat cagatacaat aaaattcgtt ggttttagtc
     4501 cacctttgaa atacgctcct actggcgtac agaagacgac aaataaatat tctggagctg
     4561 gatggacacc aatgttttca ccgaccccaa tcagtaaagg gcgcaagtat aatgtagcgc
     4621 ctgttccata gggcggcaca aaatcttcat ttgcttttac cacttgcttg acagcatcaa
     4681 cgaacatctt ttcagggacc attggcatca gcaatcgtct agcactttga ttcatccgtc
     4741 gactattttg ttcaggtcga aagagattga ccttgccgtc ttttcgacga taagctttta
     4801 gaccttcaaa acattgctga ccataatgaa gcgctggaga gccttcatga atcgtcaggt
     4861 aattatcttc tgtcaattct ccttcttccc attgtccgtc tttccatttg gcaataaaac
     4921 gccaaggagt tttgatataa gaaaaaccta gattgttcca atcgatttgc atatacttac
     4981 caccttcgtt attttctcca cattttatca ttatccatat tatttgtcat tttattttca
     5041 gcttttttcc ttcattatag ttatttttgc tatttccttt tttgaaaact tcttggtaca
     5101 atacagaaag aatcaaattt tagaaaagag gattttatgt tttcattatt agcaactgcc
     5161 ggtacgacag actcttctgg cgagacattg acagatcaag ctgtcagcaa agtaaatgcg
     5221 tttcagcggt tttgggacgg gattgattgg gacgccattt tggctgcctt tatccaaaaa
     5281 agtttatatc tgattttttt gatcgtcctt ttttggattc ttcaccgtgt gggaaaatat
     5341 ctgattgata agtcttttaa tcagtattct aaaaaagcca ttctaaacga gagccgctta
     5401 aagactttac atagtctttt aaatactatt tttcattata cccttggttt cttttttatc
     5461 tatgcaattt tatctgctat cggtgtgccg atcggatcac ttttggctgg tgccggaatt
     5521 gcaggtgtgg caatcggtct tggtgcccaa gggttcatga gtgatgttat taccggattt
     5581 tttattatca tggaacaaca gatggatgtt ggggactaca tcaagctagc aaacttaacg
     5641 attgaaggaa ccgtcgtatc tgttggtatc cgtacccttc agctgaaagc aacggatggt
     5701 actgtccatt tcatcccgaa tcgaaacatc accacgatca gcaatctctc tcgtgcaaat
     5761 atgcaagtac ttttagatat tcggattgtt cctgaagaag gatatgacaa aatctatgag
     5821 atcattgatc gagtaaatcg tacattagct gaaaaatacc aagaagagct ccaaactgaa
     5881 ccttctatct ttggattagt ggatcttgga aacagcaatt ttgcgattcg tacggtctgc
     5941 tacgcattga acggaaaaca atatgcctta aaagaagagt tcttgagctc ctatgtcaaa
     6001 gaattgacag cttctggttt cacgattccg aacagtccaa ttgctgtcgg aaaataattt
     6061 ggacaaacga tgcaacgaat ctcactaaaa agagcgtggg acaaaagtaa agaacacttt
     6121 tgtc
//
LOCUS       DACGLE010000110         6044 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0534
            SAMEA3304449-rid9560453.denovo.116, whole genome shotgun sequence.
ACCESSION   DACGLE010000110 DACGLE010000000
VERSION     DACGLE010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304449
            Sequence Read Archive: ERR985064
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6044)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6044)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702225.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702225.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 20:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,045
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 27 of 67
            Pseudo Genes (incomplete)         :: 25 of 67
            Pseudo Genes (internal stop)      :: 19 of 67
            Pseudo Genes (multiple problems)  :: 4 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6044
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304449-rid9560453.denovo.116"
                     /strain="VREN0534"
                     /db_xref="taxon:1352"
     gene            complement(29..1501)
                     /locus_tag="IXT20_002606"
     CDS             complement(29..1501)
                     /locus_tag="IXT20_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HAQ4861016.1"
                     /translation="MLQKLLKANEFIEKNAQTVNQEYRNKFHLMAPIGWINDPNGFIY
                     YQEEYHLFYQYYPYDSIWGPMHWGHAKSKDLIHWEHLPVALAPGESYDKEGCFSGSAI
                     EKDGKLYLIYTGHNVMEGKVRQVQCLAVSEDGIHFEKYAGNPIIAEDHLASVATTEDF
                     RDPKVFQRKEDYFTVVATKTADARGQIVMFHSKDLLTWEFYSVLLEGTPEQGIVWECP
                     DLFELDGKEVLILSPIQMTPQGNAYQNTNSTVAFIGTVDWQTGKFLVENFHEIDGGLD
                     FYAPQTCENEAGQRIMTAWMQMWQRKIPTNDLGHGWSGSMTLPRELRVADNQLLQRPV
                     SSIYQVIDPQEKIEWAKTEENRFLLKDLVYDQHYLRLEINLKQATKLELHYAKGPHHT
                     LLLNYDVERECFTASREHMPYDLSGAEKEVLKKRSVTIPLENNCLTIEIFRDTSAIEL
                     FLNDRLTMSTTFYETEKGQDFLLLADSEVTFSLESGLINL"
     gene            complement(1515..2363)
                     /locus_tag="IXT20_002607"
     CDS             complement(1515..2363)
                     /locus_tag="IXT20_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006639650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAQ4861017.1"
                     /translation="MTKKFGPLMIVEYCLLFLLAALFIFPMLWMVVSSMKPEADVYQN
                     LTSFKAFLPSLNPANWFKTYQEVISRFSVGTYLLNSIFYGLTFACGSIIVNSLAGFAF
                     AKVNFSGKKIIFGALLALLIVPMETVLIPQFTIVNSLGLVNTRLAVILPAMASAFNIY
                     LFRNFFIAIPEEIIESAKLDGANIVKIFFHIMLPMSKPAIATVGVLSFISSWNDYIWP
                     LMVLTDSSKFSMQVAITTINTTQPVYINQVMAVLTIATIPLIIVYIVAQKYILQGLGG
                     SGTGIK"
     gene            complement(2360..3226)
                     /locus_tag="IXT20_002608"
     CDS             complement(2360..3226)
                     /locus_tag="IXT20_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ4861018.1"
                     /translation="MKDNLIGYGFLAPALLLLTIFLIIPVIMVIYYAFTDYYLLTPDA
                     RQFIGLENFKRLFADPIFVKSIWNTAKFVVWIIPVQLGAALGMALIINKNRKGNIFFK
                     VAFFAPVVMSLVVISILWLYLLNPNEGLVNTLFAKIGIASQPFLTSPKQAMYTLVFVS
                     AWQGAGYQMLLFLGGMQNIPQDVYEAAEIDGFSKFQQFRYITMPLLKPTAIFILLTTL
                     ISAFKLIIQPMVMTQGGPMNSTMTMVYYIYQTGFTDRMVGYSSSIALIFTTMIGLITI
                     AQRKLVKEDDPA"
     gene            complement(3269..4582)
                     /locus_tag="IXT20_002609"
     CDS             complement(3269..4582)
                     /locus_tag="IXT20_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HAQ4861019.1"
                     /translation="MKRRMLAIAAILALTAGGLAGCGSKEKSSGSSDSGEIKIWVQFS
                     DETPEGKAWAEIVNNFNDEYEGEYKAVTEYIPRSGSGGGYEDKVNAAITTNTLPDVIT
                     LDGPNTAAYAHSGVIAPLDDYLKDADMDDVLDSIIQQGTYDGKMYAFGFSESSVGVYY
                     NKSLFKDAGITEDQLPTLEDPWTWTEFQAICQKLTDKYKTPAIDMQMESGDEMLTYAY
                     TPFVWSNGGDVVNADGTKAEDYFNDKKSVEALEFIQELVEKGYTTISPIEKGFETGKY
                     AMLLSGSWTMADLEENYQEIDYGILPYPVSDATGELVSPTGSWQLAMTEKTEKKDGSA
                     AFIKFATSTESSRIMSLGNSVLPIRNSTVDLIKDEVSEGMQVLIKQNQISGHARPVVV
                     AYPQVSRAFQQAVQDISYFEENPDVQKVADSRAAEMQQVIDTSLK"
     gene            complement(4724..5695)
                     /locus_tag="IXT20_002610"
     CDS             complement(4724..5695)
                     /locus_tag="IXT20_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAQ4861020.1"
                     /translation="MKTTMKDVAALAGVGVGTVSRVINGVKVKEVTKEKVMAAISELN
                     YEPDEYARGMKTNSSNTIALILPTIWHPFFSEFAYYVEEALSQKNYKLFLCNANGSAE
                     KEKEYIQMVKQSKVDGIIGITYSDIDKYVSSNLPFVSIDRHFSEQVSYVTADNYLGGQ
                     IAAQELLTRNCKNLCYVGGFSPYPNETNNRKAGFYNICEKNKIAVKVLDMPEPISNLE
                     EQLLAFFSDNPEIDSIFTINDFMALDVIKAQKKLGKEAPKDFQIIGFDGVKLSLQKDY
                     LLSTIVQPIEEMAKVSVETLMAMILNEEPPKRVVVPVFFHAGETTKE"
     gene            complement(5821..6044)
                     /locus_tag="IXT20_002611"
                     /pseudo
     CDS             complement(5821..6044)
                     /locus_tag="IXT20_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613886.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS110 family transposase"
BASE COUNT         1827 a         1309 c         1024 g         1884 t
ORIGIN      
        1 accataaatc atcactgagc tctcccttct atagattaat taagccgctt tccaatgaaa
       61 aggttacttc actgtctgcc agcaaaagaa aatcctgtcc tttttctgtc tcataaaaag
      121 ttgtactcat agtcagacga tcatttagaa aaagctcaat agctgaagta tcacggaaaa
      181 tttcgatggt tagacaatta ttttccaaag gaatcgtcac actgcgcttt ttcaacactt
      241 ccttttcagc tcctgataaa tcatacggca tgtgttccct ggaagcagta aagcactccc
      301 gttcaacatc atagttaagc agtaatgtat ggtggggtcc cttcgcatag tgaagttcta
      361 gtttcgttgc ctgttttaaa ttgatttcca agcgtaaata gtgctggtca tatactaagt
      421 ccttcagtaa gaatcgattc tcttcagtct tcgcccattc aatcttttcc tgcggatcaa
      481 ttacttgata aattgatgaa actggccgtt gcaacaattg attatcagcc accctcagct
      541 ctctaggcaa ggtcatggaa cctgaccaac catggcccag atcattcgta ggtattttac
      601 gctgccacat ttgcatccaa gcagtcatta ttctttggcc tgcttcgttt tcacaggtct
      661 ggggggcata aaaatctaaa ccgccatcga tttcatgaaa attctctact agaaatttgc
      721 ctgtttgcca atcgacagta ccaatgaaag caacagttga gttcgtattt tggtaagcat
      781 tgccttgtgg agtcatttga ataggagaaa gaatcaagac ttctttgcca tctagttcaa
      841 acaagtctgg gcattcccat acaatgcctt gttctggagt tccctccagc aatacggaat
      901 aaaactccca agttagtaaa tctttggaat ggaacatcac aatttgtcca cgagcatctg
      961 cagttttagt agcgactaca gtaaaatagt cttcttttcg ctgaaacact ttgggatcgc
     1021 ggaaatcttc ggtcgttgca acagaagcta agtgatcttc tgcgatgatt ggatttccgg
     1081 catatttttc aaagtgaatg ccatcttctg aaactgccaa acactgaact tgtcgaactt
     1141 tcccttccat aacattgtgt cctgtataaa tcaaatacag cttgccatcc ttttctatag
     1201 cactgccgga aaaacagcct tccttgtcat agctttcacc tggtgccagt gctactggca
     1261 aatgctccca gtgaattaga tccttgcttt tagcatggcc ccaatgcatt ggtccccaga
     1321 tgctgtcgta aggataatat tgataaaaca agtgatactc ctcttgataa taaataaaac
     1381 catttggatc attgatccag ccaatcggag ccatcaaatg aaatttattc cggtattctt
     1441 gattgactgt ttgagcattt ttctcaataa actcattggc ctttaaaagt ttttgcaaca
     1501 ttcactattc actcctattt tatacccgtt ccagaaccgc caagtccctg cagaatatat
     1561 ttttgtgcaa cgatataaac aataatcagt ggaatcgttg caatggttaa aacagccatc
     1621 acttgattga tataaactgg ctgtgtagta ttgatcgtag taattgccac ttgcattgaa
     1681 aatttgctgc tgtcagttaa gaccattaac ggccaaatat aatcattcca gctggagata
     1741 aaggataaga cacctactgt ggctatggct ggtttagaca ttggcagcat gatgtgaaag
     1801 aaaattttaa caatatttgc accatctaat ttcgctgatt caatgatttc ttctggaatc
     1861 gcgataaaga aatttctaaa caaatagata ttaaacgcgc tggccatcgc tggtaaaatc
     1921 actgccaagc gagtattcac taagcctagt gaattaacga tggtaaattg cggaattaaa
     1981 accgtctcca tcgggacaat cagcagagct aacaaagctc caaagataat tttcttgcct
     2041 gaaaaattaa ccttcgcaaa ggcaaaaccc gccaaagagt ttacaatgat tgacccacag
     2101 gcaaaggtta aaccataaaa aatactgttt aataaataag ttcccacact gaaccgggaa
     2161 ataacttctt gataagtttt aaaccaatta gctggattca aactaggtaa aaaagctttg
     2221 aaggaagtta aattttgata gacatctgct tcaggcttca tagaggaaac taccatccac
     2281 agcattggga aaatgaataa tgccgctaaa aggaacagca aacaatattc tacgatcatt
     2341 agcggtccaa atttcttagt catgctggat catcctcctt taccaatttt ctttgagcaa
     2401 ttgtaattaa accaatcatg gtggtaaaga ttaacgcaat ggagctggag tagcctacca
     2461 tacggtctgt aaatccagtt tggtaaatgt aataaaccat tgtcatagta gaattcattg
     2521 gtccaccttg agtcattacc attggctgaa tgattagttt aaaggccgaa atcaatgtgg
     2581 taagtaaaat aaaaatcgcc gtgggcttta ataatggcat ggtgatatag cgaaattgct
     2641 gaaacttaga aaaaccatca atttcggctg cctcataaac gtcttgcgga atgttttgca
     2701 ttccccctaa aaacagcagc atctgatatc ctgcaccttg ccatgccgat acgaaaacaa
     2761 gcgtatacat tgcttgtttg ggactagtca aaaaaggctg cgaagcaatt ccaatctttg
     2821 caaatagcgt attaaccaga ccttcattag gatttaacaa gtacaaccat aaaatcgaaa
     2881 taacgactaa ggacatcacg actggcgcaa aaaaggctac tttaaaaaag atatttcctt
     2941 tacgattttt gttgataata agtgccattc ctaaagctgc ccctaattgg accggaataa
     3001 tccaaacaac aaatttagcc gtgttccaaa tactttttac aaaaattgga tcagcgaaca
     3061 gtcgcttaaa attttctaag ccgataaatt gtctagcatc tggcgttaac aaataataat
     3121 cagtaaaggc ataataaatt accatgatta cgggtatgat cagaaaaatc gtcagcagca
     3181 gcaaagctgg cgctagaaat ccgtaaccta tcaaattgtc tttcattgtc ggttttttta
     3241 acacttgatt ggcccccttt agaaacttct attttagtga agtatcaatt acctgttgca
     3301 tttcagcagc gcgactatct gctacttttt gtacatctgg gttttcctcg aagtagctaa
     3361 tatcttgaac tgcttgttgg aatgctctag atacttgcgg ataagctaca accactgggc
     3421 gggcatgtcc agaaatttgg ttttgcttaa ttaatacctg cattccttcc gacacttcat
     3481 ctttaattaa atcaactgtt gaatttctga taggtagaac actgttccct aaactcataa
     3541 tacgactaga ttctgtactg gttgcaaact taataaaggc cgcagaacca tcctttttct
     3601 ctgttttctc agtcattgct aactgccagc tgccggttgg tgaaactaac tccccagttg
     3661 catctgaaac ggggtaaggc aaaataccat aatcaatctc ttgatagttt tcctctaaat
     3721 ctgccattgt ccaagaaccg ctcagcagca tggcatattt gccagtttca aatccttttt
     3781 caataggact gattgtggtg taaccttttt caaccaattc ttgaataaat tccaacgctt
     3841 caacggattt tttatcgtta aaatagtctt cggctttggt tccatcagca ttaacgacat
     3901 cgccgccatt cgaccataca aatggtgtat atgcgtaggt cagcatttcg tctcccgact
     3961 ccatttgcat atcaattgca ggcgttttgt atttatcagt cagcttttgg caaattgctt
     4021 gaaattccgt ccaagtccaa ggatcttcta acgtcggcaa ttgatcttct gtaattccag
     4081 catctttgaa caacgactta ttgtaataga cacccacact ggactctgag aaaccaaacg
     4141 catacatttt gccatcgtag gttccttgct gaataatgct gtctaataca tcatccatgt
     4201 ccgcatcttt caaataatca tctaatggag caatgactcc tgaatgagca taagctgcag
     4261 tattcggtcc atctaacgta ataacatcag gaagggtatt ggttgtaatt gcagcattta
     4321 ctttatcctc atatccaccg ccgcttccgc tacgaggaat atactcagta acagctttat
     4381 attcaccctc gtattcatca ttaaaattgt tcacaatctc tgcccaagct tttccttcag
     4441 gcgtttcatc agaaaactga acccaaatct taatttctcc tgaatcactg ctgcctgaac
     4501 ttttctcctt actgccacat cctgcaagtc ctccagctgt taatgctaaa attgctgcaa
     4561 ttgctaacat tctccttttc atcgatgttc ctcctatttt ttgaaacgtt tccgttttat
     4621 tctccaatta attggtaacg tttccaatta attggtttta agatatcatt gaaaccactt
     4681 tctgtcaacc tatctcaaaa aaaaatcgtt ataaaccggt tttttactct ttagtcgttt
     4741 ctccagcgtg aaagaaaacc ggtactacca cacgcttagg cggttcttca tttaaaatca
     4801 tcgccatgag tgtttctact gaaaccttgg ccatttcctc tatcggctgt acaatcgtcg
     4861 atagtaaata gtctttttgc aacgatagct tgactccatc aaaaccaata atttgaaaat
     4921 ccttaggtgc ttccttccct agtttttttt gggcttttat cacatccaaa gccatgaaat
     4981 cattgatggt aaaaatgcta tcaatttcag gattatccga aaaaaaagcg agcaattgtt
     5041 cctccaaatt gctaattggt tcaggcatat ccaatacctt cacagctatt ttatttttct
     5101 cacaaatatt gtaaaaacca gctttacgat tgtttgtttc gttagggtaa ggtgaaaaac
     5161 ccccaacata gcacaagttt ttacagttac gagtaagtag ttcttgagcg gcaatttgcc
     5221 cacctaaata attatctgcc gttacataag aaacctgttc cgaaaaatgg cggtctatac
     5281 tgacaaatgg taaatttgat gatacatatt tatcaatgtc tgaataggta atacctataa
     5341 ttccgtctac tttactctgc ttgaccattt ggatatattc tttttctttt tccgcactgc
     5401 cgttcgcatt acacaaaaaa agcttataat ttttttggct gagagcttcc tctacatagt
     5461 aggcaaactc ggaaaagaag ggatgccaaa tcgtaggtaa aataagagct attgtattgc
     5521 tgctattcgt tttcatacct ctggcatatt catcaggctc ataattaagc tcactaattg
     5581 cagccattac tttttctttg gtaacctctt tcacctttac tccatttatt actctagata
     5641 ccgtacccac accaactcct gctaacgcag caacatcctt catagtagtt ttcacttcac
     5701 gaacaactcc tctcaaaaga atttcaggta ttattatacc aaaagaagaa aacgattcca
     5761 aattagcagg aatattttta ttttaaaata gatattagac gtgaagcttt tcgtaaaaaa
     5821 attttcgtat tcagatttac cggattgact ctgtcgccaa tataggccgg catactttgt
     5881 caattttatt tcatcagaaa aacgttcaat tggaccaatt tcagcgagta ttccagcagc
     5941 ctaaacagga ccaatacctg ggatactggt taaacattga tactccggta gcaccacgac
     6001 taagtcgtca atggctttat ctaagtcttt aattaacccg tggt
//
LOCUS       DACGLE010000111         5985 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0534
            SAMEA3304449-rid9560453.denovo.117, whole genome shotgun sequence.
ACCESSION   DACGLE010000111 DACGLE010000000
VERSION     DACGLE010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304449
            Sequence Read Archive: ERR985064
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5985)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5985)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702225.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702225.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 20:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,045
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 27 of 67
            Pseudo Genes (incomplete)         :: 25 of 67
            Pseudo Genes (internal stop)      :: 19 of 67
            Pseudo Genes (multiple problems)  :: 4 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5985
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304449-rid9560453.denovo.117"
                     /strain="VREN0534"
                     /db_xref="taxon:1352"
     gene            complement(50..1516)
                     /locus_tag="IXT20_002612"
     CDS             complement(50..1516)
                     /locus_tag="IXT20_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HAQ4861021.1"
                     /translation="MEKQQDKGFFGQPKGLSTLFFTEMWERFSYYGMRALLIYYMYDT
                     VANGGLGLPRATALAIMSIYGSLIYMSSIVGGWVSDRVLGAKRTVFFGGVFIMLGHIV
                     LALPFGVSTLFVSMALIIIGTAFLKPNVSGMVGHLYSKTDLRRDAGFSIFYMGVNLGA
                     LLAPLIVGTIGQEYNYHLGFSLAAIGMFFGLLQYIIQGRKTLDGVGTTPPNPLTTEER
                     KGFIRNLIIALVVIAAVFGGAQLTGHLTIDFFVNTISVLGIILPLYYFIKMLTSKDVT
                     AEEKPRVWAYIPLFLAAIIFWSLQEQGSSILALFASERTQSTLFGVTIQASWYQSLNP
                     IFIFILTPVFVTMWTKLGERQPSTVVKFSLGLLFAGLSYLLLMLPGMLYGTSGKVSPL
                     WLLGSFFIVVIAELCLSPVGLSVTTKLAPRAFESQTIAIWFLADASSQAINAQIARFY
                     TPGTESAYFGIIGAVAIVGGILLFTVKEPIKKLMGSIH"
     gene            1903..2499
                     /locus_tag="IXT20_002613"
     CDS             1903..2499
                     /locus_tag="IXT20_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HAQ4861022.1"
                     /translation="MTTFTDTLKNRRSIYHLGRNVSLSNEELTTLIKEAIKESSTAFN
                     AQSTRAVILFGDAHEKLWEITEEALRPLTPAEAFPNTQNKLAGFKNGYGTVLFFKDTD
                     VVKGLQEQFELYADNFPDWSEQSNGIATANTWVALVDKGLGANLQHYNPVIDEAVAKE
                     WNIPSNWKLRSQLVFGSPETPAGEKEYMNDADRFRVFG"
     gene            complement(2557..3039)
                     /locus_tag="IXT20_002614"
     CDS             complement(2557..3039)
                     /locus_tag="IXT20_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4861023.1"
                     /translation="MVTFFTVLLFLFVVVMFLFLWLVRKEIIYRTVRNRWVYLVIPFL
                     VSLVIWYTLISHPTADELAKGILSAMIFISFLLDSRGITEEGLVLNSFDKKGVPFSEI
                     NKIVLYQPKGSKIVKMNFFRNGWRGPMLKFSASLEELVPFLSQRLNDEAEIDIMIDPE
                     "
     gene            complement(3145..3342)
                     /locus_tag="IXT20_002615"
     CDS             complement(3145..3342)
                     /locus_tag="IXT20_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4861024.1"
                     /translation="MTEEMINLGEQYACKPIGFTKTVIGEVVSKMTNCAVVKVAQCAA
                     EDQELLDEKASMVVAKYDTFE"
     gene            3637..4395
                     /locus_tag="IXT20_002616"
     CDS             3637..4395
                     /locus_tag="IXT20_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase C39"
                     /protein_id="HAQ4861025.1"
                     /translation="MKKKTFKKLLFLGGTAALLLSGTSYYLHIHSPEHTMNTAQKSST
                     QKTKKTKEKAEVLLDVPLESQFDSPSLENGCEVTSLSMLLSFYGYETTKNQLAEQLDY
                     VPVFNADGTHGDPNEGFVGDISGGDWAMVVYVPPVASLAQQIVQTDYHTFPKTDAQLD
                     DIKKALSKGNPVWTSVTINFEVPDETDFMTWTTNNGEVRVTPLVHACVVTGYDKENIY
                     VNDPYGVKNRAVPINDFSAIFTAMGGQMLTLEKS"
     gene            4455..4883
                     /locus_tag="IXT20_002617"
     CDS             4455..4883
                     /locus_tag="IXT20_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4861026.1"
                     /translation="MEKFTHKKMDPNEIPIIFVRDCKGNVQGKVSINEWNERRRPATL
                     NELEIKLYRQSLVYYADQEYEKATDLLKFLIARTEYTRFEYIERLANIYHIMNEPVKE
                     YQLLDTVLSVAELIALPAGLEKKLVRRLLRVKQQLSDQEK"
     gene            4929..5001
                     /locus_tag="IXT20_002618"
     tRNA            4929..5001
                     /locus_tag="IXT20_002618"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4962..4964,aa:Gln,seq:ctg)
     gene            complement(5151..5522)
                     /locus_tag="IXT20_002619"
     CDS             complement(5151..5522)
                     /locus_tag="IXT20_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HAQ4861027.1"
                     /translation="MKVIKYLIGLAAISGILLFGLKYYTEGSYGEIPGILDQLNPLVE
                     KGEVYVKTQKPEEVNEYGTARYVQKAADANGKERTVEFNGLSVLKENHYLKISNKGAH
                     IETYEEVPREKVPEKALAIIG"
     gene            5737..5922
                     /locus_tag="IXT20_002620"
     CDS             5737..5922
                     /locus_tag="IXT20_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4861028.1"
                     /translation="MNQEELFQKVKEMIKNGNFDGAKRFIEEHKEQLGPYKEKAQNLL
                     KDVNIDSVKNKFKNLFK"
BASE COUNT         2021 a         1157 c          991 g         1816 t
ORIGIN      
        1 aaaacaggaa ctgctgcaaa taagcaattc ctgtttttat gttcattgat taatgaatag
       61 agcccattag ttttttgatt ggttctttca cagtaaatag caagatacca ccgacgattg
      121 ctactgcacc gataataccg aagtaagcag attctgttcc cggtgtgtag aagcgtgcaa
      181 tttgtgcatt gattgcttgt gaagaagcat cagctaagaa ccaaatagcg attgtttgtg
      241 attcaaatgc tcgtggtgct aattttgttg tcacagacaa accaactggt gaaagacaca
      301 attcagcaat aaccacgatg aagaaacttc ccaacaacca caatggactt acttttccac
      361 ttgtaccata aagcataccc ggcaacatca atagcaagta cgataatcct gcaaataaaa
      421 gtccaagaga gaatttcact actgtagatg gttgacgttc accaagtttc gtccacatcg
      481 ttacgaatac tggtgtcaga ataaagatga aaattggatt caatgattga taccagcttg
      541 cttggatcgt tacaccaaat agcgtagatt gtgtacgttc actagcaaat aaagctaaga
      601 tagatgaacc ttgttcttgt aatgaccaga aaatgattgc tgcaaggaat aatgggatat
      661 aagcccatac tcttggtttt tcttcagcag ttacatcttt tgaagtcagc attttgataa
      721 agtaatacaa cggtaagata atccccaata cactgattgt attgacaaaa aagtcaattg
      781 tcagatgacc agttagttgt gccccgccga atactgcagc gataacaaca agtgcgatga
      841 tcaagttacg gatgaatcct ttacgttctt cagttgtcaa aggattcggc ggtgttgtac
      901 ccactccgtc taatgtcttg cgtccttgga tgatgtattg taataatcca aagaacatcc
      961 caatagcagc taaagagaac cctaaatgat agttatattc ttgaccgatt gtaccaacga
     1021 ttagtggtgc taataatgcc cctaagttga cacccatgta aaaaatcgaa aatcctgcat
     1081 ctcttcgcaa gtcagtcttt gaataaagat gtccgaccat gccagaaaca tttggtttca
     1141 aaaaggcggt tccgataata atcaatgcca tcgaaacaaa caatgtactt acaccaaatg
     1201 gtaaagccaa gacgatgtgg ccaagcataa tgaaaactcc accgaagaat acagtgcgtt
     1261 ttgctcctaa cactcggtca gatacccatc cgccgacgat actagacatg tagatcaaag
     1321 atccgtagat cgacatgata gctaatgctg ttgctcgtgg taatccaagt cccccgttgg
     1381 caactgtatc gtacatataa tagataagta atgcacgcat accgtaataa ctaaatcgtt
     1441 cccacatctc agtgaagaac aaagttgaca atcccttcgg ctgcccgaag aagcccttgt
     1501 cttgctgttt ttccataatc tataccccaa ttctttaaat tttcagaaaa tattatacat
     1561 ctttaccgaa aaatttaact aaaaaactta tttaagaaat ataaactaat tttctataaa
     1621 agcaagtaat ttttaatggc aatatcataa aaaaaagaag atagagattt aataattata
     1681 tatcagctgt ttttttcaaa taagcaaaaa ttcctaaaac acaacaatga aagcgataac
     1741 taagaaaatc cttacataac gatttttctt attaaaattg ttttttgaac ttcgcttatt
     1801 ttttgtgagc atatctgttt acttactttt tgtaaggtgt tatacttcat ttcgtaatca
     1861 attaaaaaaa tgcaaaacgc aaatcaaagg agaaatttta ttatgacaac ttttactgac
     1921 actttgaaaa accgccgttc aatctatcat ctaggacgta atgtttcact ttctaatgaa
     1981 gaattaacaa ctttgatcaa agaagcaatt aaagaaagct caactgcttt caatgctcaa
     2041 tcaacacgcg cagttatcct tttcggagat gcacatgaaa aactttggga aatcactgaa
     2101 gaagcacttc gcccattgac tccagcagaa gctttcccaa atacacaaaa caaattagct
     2161 ggtttcaaaa atggttatgg tacagtatta ttcttcaaag atactgacgt ggtaaaaggt
     2221 ctacaagaac aatttgaatt atatgcagac aacttcccag attggtcaga acaatcaaac
     2281 ggaatcgcaa cagctaatac ttgggtagca ttagttgata aaggattggg cgcaaacttg
     2341 caacattaca acccagttat tgacgaagca gttgcaaaag aatggaatat cccttcaaac
     2401 tggaaactac gttcgcaatt agtattcggt tcaccagaaa caccggctgg ggaaaaagaa
     2461 tacatgaacg atgccgatcg cttccgcgta tttggataat tttatttgaa ataaaaaaca
     2521 gaggttgtga caaacatctt gtcgcaacct ctgtttttat tctggatcaa tcataatatc
     2581 aatctctgcc tcatcgttta agcgttggga caaaaatgga actaattctt ctaatgatgc
     2641 agaaaatttc agcatcggtc ctcgccatcc gtttcggaaa aaattcattt ttacgatttt
     2701 agaacctttt ggctgatata atacaatttt attgatctca gaaaaaggaa cacctttttt
     2761 gtcaaagcta ttcaaaacaa gtccttcttc tgtgattcct ctactgtcca acaaaaaact
     2821 aataaaaatc attgctgaca agatcccttt tgccaattca tctgctgtag gatgactgat
     2881 gagtgtatac caaatgacta gtgacactaa gaagggtatc accaagtaga cccatcgatt
     2941 tcgcacagtc cgataaataa tttcttttcg aaccagccaa agaaataaaa acatcacaac
     3001 aacgaataaa aacaatagaa ccgtaaagaa tgttaccatg acaactcgcc tcgctttttc
     3061 ttctccagaa aataggaaaa gaatactgat ttaccttctc aaagaaattg gacttgcagc
     3121 acgccgcacg tccaattgtt ttctttattc aaatgtatca tattttgcaa caaccatgct
     3181 tgctttttca tctaataatt cttggtcctc tgcagcacat tgggcaactt tgaccacggc
     3241 gcagtttgtc atttttgata caacttcacc aatgaccgtt tttgtaaaac caattggttt
     3301 gcatgcatat tgttctccta aattaatcat ttcttccgtc atcaaaatcc tcctaaactg
     3361 ctggaacttt tctccacatt accactgcta tttctttgga gatttaactc attgattttt
     3421 atttacagtt aaaaatcaac cttacaggca ttatagcgta ctgttttcgt tttcacaatt
     3481 aaaacagctg ttgtatttag aagaattttt ttcaaaaaat taaaacatat tccttaaaaa
     3541 ctaattattt cctacatatt aaaatataat acttccattt ccaataaaaa tacatttata
     3601 ataggaaaga taaacagctg aaaagggaga tttcaaatga agaaaaaaac gttcaaaaaa
     3661 ctattgtttc tagggggcac agctgctctt ttattaagcg gtacaagtta ttacctacat
     3721 atccattctc cagaacatac aatgaacact gcacaaaaaa gttcaacaca aaaaacgaaa
     3781 aagacaaaag agaaagcaga agttttacta gatgttccat tagagagcca gtttgattct
     3841 ccttctttag aaaatggctg tgaagtcact tcgctttcta tgcttttatc tttttatggt
     3901 tatgaaacaa ctaaaaacca gttagctgag caattggatt acgtccctgt tttcaatgca
     3961 gatgggactc atggcgatcc taatgaagga tttgttggtg atatcagtgg cggagactgg
     4021 gctatggtcg tctatgttcc cccagttgcc tcccttgctc agcaaatcgt ccaaacggat
     4081 tatcacactt ttccaaaaac cgatgctcaa ctcgatgata tcaaaaaagc actatctaaa
     4141 ggaaatcctg tctggacttc tgtgactatc aattttgaag tcccagatga aactgatttt
     4201 atgacttgga ccacaaataa tggagaagta agagtcactc ctcttgttca tgcttgtgta
     4261 gtcactggat atgacaaaga aaatatttat gtcaacgatc cctatggtgt aaaaaatcga
     4321 gcagtaccga tcaacgattt ttcggcgatt ttcactgcaa tgggtggaca gatgctaact
     4381 ttggagaaaa gctagtaatt caaacaaaaa tcccttatac tcaagatacc aaacctttga
     4441 agggagcgta gataatggaa aaattcacac ataaaaaaat ggatccaaat gaaattccga
     4501 ttatttttgt ccgtgactgc aagggaaatg ttcaaggtaa agtctctatt aatgagtgga
     4561 acgagcgtcg cagacctgct accctcaatg aactggaaat caaattgtat cgtcaatctc
     4621 ttgtttatta cgcagatcaa gagtacgaga aagccaccga tttattgaaa tttctaattg
     4681 ctcgaacaga gtatactcgc tttgaatata ttgaacgttt agcaaacatc tatcatatta
     4741 tgaatgaacc tgtcaaagaa tatcagttat tggacaccgt cttgtcagta gcagaactga
     4801 ttgcccttcc tgctggatta gaaaagaaac tagtacgccg ccttttacga gtaaagcaac
     4861 aactatccga tcaagaaaaa taacttgcgt ttttttagga aagtgctatg attattttgt
     4921 tgaataaatg ggatgtagcc aaactggtaa ggcacttgac tctgaatcaa gcaattgtag
     4981 gttcgaaccc tgccatccca atttgatgtg cttgctgaaa aaatgaaccc cctgtaaaag
     5041 aacaatcttt ttgcaggggg attattgtat tatttttact acttgtattt agtttacata
     5101 attaaattat gaataataaa atcgggcgta ttcttaatac aaaaactgat ttacccaata
     5161 attgctaatg ctttctccgg aactttttct ctaggaactt cctcatacgt ttctatgtgt
     5221 gctcctttat tgctaatttt caaataatga ttttctttta acacagaaag accattaaat
     5281 tcaaccgttc gttctttccc attggcatca gcagctttct gcacatacct tgctgtacca
     5341 tattcattta cttcctctgg tttttgtgtc tttacataga cctctccttt ttcaactaat
     5401 ggatttagct gatcaagtat accagggatt tctccataac ttccttcagt ataatatttc
     5461 agtccaaata gcaaaatacc actaatcgcc gccagtccaa tcaaatattt aatcactttc
     5521 atctttctac ctccttgtta tatattcagt atatgggtca gttaaagaaa ttccatccgt
     5581 cccaagttgg agatttttct cctactttag aagcgagaat cgagtgtttt tttagtaaaa
     5641 atgatcgtta tttgatatta ttgacgtgtg gataaatcag tttttctcgt tttgtccatg
     5701 taaaaaatca tgttgatagc aaaggatgtg ttcaaaatga atcaagaaga attatttcaa
     5761 aaagtcaagg aaatgattaa gaacggcaat ttcgatggcg caaaacgatt tatcgaagaa
     5821 cataaagaac aattaggacc ttataaagag aaagcccaaa atcttttaaa agatgtaaat
     5881 atcgacagtg tcaaaaacaa atttaaaaat ctattcaaat aagtaaaact ttcacttttg
     5941 taaaatcaaa ggaggacaat gctgctgcat cgtcctcctt tggtt
//
LOCUS       DACGLE010000112         5807 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0534
            SAMEA3304449-rid9560453.denovo.118, whole genome shotgun sequence.
ACCESSION   DACGLE010000112 DACGLE010000000
VERSION     DACGLE010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304449
            Sequence Read Archive: ERR985064
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5807)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5807)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702225.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702225.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 20:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,045
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 27 of 67
            Pseudo Genes (incomplete)         :: 25 of 67
            Pseudo Genes (internal stop)      :: 19 of 67
            Pseudo Genes (multiple problems)  :: 4 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5807
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304449-rid9560453.denovo.118"
                     /strain="VREN0534"
                     /db_xref="taxon:1352"
     gene            complement(1062..1460)
                     /locus_tag="IXT20_002621"
     CDS             complement(1062..1460)
                     /locus_tag="IXT20_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4861029.1"
                     /translation="MRLRRNKNYTTIILEDFTDQELLIVGKTKADFIEKGNASIVKAS
                     KIAVEKYFDSLEESAKEIFNGEKAGSYELDGIRFSLIPTTENIIPKKLYYIFSSEKHT
                     SMKPCIFDWQWLIDAVLDVKCSKYNITLGC"
     gene            1676..2731
                     /locus_tag="IXT20_002622"
     CDS             1676..2731
                     /locus_tag="IXT20_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParM/StbA family protein"
                     /protein_id="HAQ4861030.1"
                     /translation="MKEPLLFSVDLGNKSVKEMSTHVSPLSLPSRILNKNIINSGRFS
                     NIGSSLKRSLNVSEYSLVNTTESYYFGKDIHELGKDDYVIESLGFGISRYKQETYQNI
                     LSFSLANLARLEDQSDDVLTANLVLGLPSSDYTETIVKYVMQQATRQHSVEVDGKAYN
                     INVDTVWVLPQPIGTLYNLLLADDGSVQDETLISQKIGFVDIGGGTKLFDVVQNFEID
                     ENNREQKDTGIFTLYNRIVNMLDLPISPNAFQLEKIIREGKEKGVYLFKPSGNESFDI
                     SEVVEREIKTYTNMVVAEVLATFKNFTTLDKVVFTGGGASIIDKQLVKDKIPHTLFVE
                     DSEFSNVKGFYKFGLTQ"
     gene            2751..3104
                     /locus_tag="IXT20_002623"
     CDS             2751..3104
                     /locus_tag="IXT20_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4861031.1"
                     /translation="MNKGKSERYVVIINREKVSELPLAEFLESEYETLGVKYSQTIKK
                     ALNLYRRVKNNEVTIIEAGNDRNDSKVAEVKNKPTDHNPIQQAHPSTVTSDHSAKKEK
                     PMLKGFNAPSNKDLL"
     gene            complement(3286..3582)
                     /locus_tag="IXT20_002624"
     CDS             complement(3286..3582)
                     /locus_tag="IXT20_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4861032.1"
                     /translation="MIEVKENRFKKFAEAEVEEVPEVVEAVEEPKQTPKETTSSLVAK
                     LKSDSSEKKKNYQFTLQPSQRKKLSKISEKLGIRSDSALLGEMIDNLYDEVFGE"
     gene            complement(3586..4404)
                     /locus_tag="IXT20_002625"
     CDS             complement(3586..4404)
                     /locus_tag="IXT20_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAQ4861033.1"
                     /translation="MEKKLSIITGNIEKGGTGKTTTIYNLGGYAVENLGMKVLYIDED
                     KSQNLSKRFPDAFSKAKRENTINHLYLHGSAEPLAINENMDILVAGEDLKQVEMQMRD
                     MPNNRTLLFRWIVVNYEQLKEKYDLILIDTHNDAGLLAQNAWAVSDLVVGISDPSKDG
                     FEALLKLGKDIDALAKQLIDIKTGETLMETKYATIGSRIKHNTNSSREFLELVPTLSN
                     YLGYVQEKELLNATSLEGKNIAEFAKDKNIYRKNKDFFESTFTLYNKIIDTAQE"
     gene            4630..4851
                     /locus_tag="IXT20_002626"
     CDS             4630..4851
                     /locus_tag="IXT20_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HAQ4861034.1"
                     /translation="MPVISIRVSKEEKDFLEKVAEFNGDNISEFMRSNSLQTAETLVD
                     FATYKQLMKEHEENDQSISHEDMLKELGL"
     gene            4853..5119
                     /locus_tag="IXT20_002627"
     CDS             4853..5119
                     /locus_tag="IXT20_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HAQ4861035.1"
                     /translation="MTYQVRYEREAQKALKKMDKFQAKVILNWIEKNLVGTDDPRKHG
                     KGLTANKSGYWRYRVGAYRIIADISEHEVTILILSIGHRSEVYK"
     gene            5232..5771
                     /locus_tag="IXT20_002628"
     CDS             5232..5771
                     /locus_tag="IXT20_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4861036.1"
                     /translation="MYNISVNKLINFQKERFFMGWYGDYSSANEVIEEKKGYAKNGLE
                     IVGTKVTKTYGIILFKDAEGTYDIDSFIFRDEMYKPIYFLNDSPAYFKKIPKKWLKFF
                     VGDQEKMVKLQLDRIKEESNKPKLDDLLVAGKTYSIWDGSYVAVYQYKLKRSFIFRLQ
                     DGSLTRFTRLSAGDVKEVT"
BASE COUNT         2047 a          961 c          864 g         1935 t
ORIGIN      
        1 ttcctttttc atgttatttt tatccagaaa ccgatcaagt ttttacctat tcccaaaatc
       61 catttattcg ttattttttt cactgattaa gaataattat ttgatgctta aattagctaa
      121 aaaagcgatt tgttactgat ataatcatga tattgcagct gaattaggcg gaaagataaa
      181 atgttcgcaa aatcaaagta tcaattatct tgttttttta taaatttgta agtaaaaatc
      241 taaaaaagtt ttcttttaat tggtatatgt attaaaatca catttaatca cccataaagt
      301 atgcgctttc atttttttgg atgagattaa tatcaataaa taacgatgta aaaatcattt
      361 attatgctct tttataagga tattaattga tctaatttga aaaaaatcaa aatttaaatg
      421 ttttaatatt acaaataaaa tcatgttatt gtaaaatggt ataaaaaagc taaatattct
      481 atttttgtta ttttattagc aggaaaattt aaatagtttt acaagaatcc gtagaaatat
      541 attggttttg ccttcagatt aatactggat catattaatc tgaatataat atatgaacta
      601 ataaatagct taacctcgct attttgtttt ttctgatcac tatctataca gcattagtaa
      661 atagaatttt aaaaataaaa aaattaagac aaaaaagtat caacataaac atatacatat
      721 acacgtttat gttgattcac ttaatttgtc ctttaaaaag cacttatttt aatataatgt
      781 tattttataa tcttattaca agctgattgt taggcgttgt cctttcatta catttattaa
      841 cggttatttt attgtttaat ttattaatta tttttgatct aaaacttagc ttaacaaata
      901 aataattaac caaccgccag ataaaaatgc gatcattgct aatacataag caaagataaa
      961 aatatgttcg atcagcttaa accctgatgc gattaagcag accaagtaaa atagaaattc
     1021 tcttacagat aatggtaatt ttctaactag catactcata attaacaccc caaagtaata
     1081 ttgtattttg aacattttac atccaataca gcatcaatca accattgcca atcaaaaata
     1141 caaggcttca ttgaagtatg tttttcagat gaaaagatat aatataattt cttcggaata
     1201 atattttcag ttgttgggat caagctaaat ctaataccgt ctaactcata gctaccagct
     1261 ttctctccat taaaaatttc cttagcactt tcttctaaag aatcaaaata tttttcgaca
     1321 gcaatctttg aagcttttac tattgaagca tttccctttt cgataaaatc tgcttttgtt
     1381 ttaccgacaa taagtaattc ttgatcagta aaatcttcta aaataatggt agtgtaattc
     1441 ttatttcttc tcaatcgcat caggatcact ccttaatata atttatgttt ttataatatc
     1501 ataataagat aaatttgcaa gcatttattt acaccttttt atttacactt tattataatt
     1561 aattagactt ttatgattgc atttttttct tataaacagt tgcaactaat agtaaatagg
     1621 tgtaaactat tttcgtaccg aaataccata aacacaaaca gaaagaggtc ataccatgaa
     1681 agaaccatta ttgttttccg ttgacttagg gaataaatca gtaaaagaaa tgagtacaca
     1741 cgtttctcca ctctcacttc cctctcgtat tttgaacaaa aatatcatta attcaggacg
     1801 tttttctaat ataggttcct cattaaaacg atcactaaac gtttctgaat atagcctagt
     1861 aaacactact gaaagctatt actttgggaa ggatatacac gagctaggaa aagatgacta
     1921 cgtgatagaa tcactaggtt ttgggatcag cagatacaag caggaaacct accaaaatat
     1981 cctatctttc tctttagcta acctagcacg tttagaagat caatctgatg acgttttaac
     2041 cgctaatcta gttttaggtc tgccttcatc cgactatacc gaaacaatcg tcaaatacgt
     2101 gatgcaacaa gccacacgac aacattcagt tgaagttgac ggtaaagctt ataatataaa
     2161 cgtagatacc gtttgggtac tgccacagcc gattggaaca ctatataacc tcctacttgc
     2221 tgatgatggt agtgtacaag atgaaacatt gatcagtcag aagatcggtt ttgttgatat
     2281 tggtggtggc acgaaacttt ttgatgttgt ccaaaatttt gaaattgacg aaaacaaccg
     2341 tgaacaaaag gataccggta tttttacttt atataatcgt atcgtaaata tgcttgacct
     2401 tccgatcagt cctaatgctt tccaacttga aaagattatc cgagaaggca aagaaaaagg
     2461 cgtatatcta tttaaaccaa gcggaaatga aagttttgat atttcagaag tagttgaaag
     2521 agaaataaaa acatacacca atatggttgt tgcagaagtt ttagctacat tcaaaaactt
     2581 cacaacctta gataaagttg tctttactgg tggtggggct tctattatcg acaaacaact
     2641 tgtaaaagat aaaattccgc ataccttgtt tgttgaagac agtgaatttt ctaatgtaaa
     2701 aggcttttac aagtttggtt taactcaata aaacagcgaa ggagatcaac atgaataagg
     2761 gaaaatcaga gcgttatgta gttataatca atagagaaaa ggttagcgag ttacctttag
     2821 cagagtttct tgaatctgaa tatgaaacac taggcgttaa atactctcag acgatcaaaa
     2881 aagctttgaa tctatatcgg cgtgtaaaga acaatgaagt tacaattatt gaagcaggca
     2941 acgatagaaa cgatagcaaa gtagctgaag taaagaataa accaactgat cataacccta
     3001 ttcaacaagc gcatccttca acggttacat ccgatcattc agcaaagaaa gaaaagccaa
     3061 tgctaaaagg gttcaatgca ccaagtaaca aagacttact ttaaaaataa aaaggcgagg
     3121 ttacaatacc tagccttttt attatgtaca aatacaaaca taaacatata cgtatacatg
     3181 tatataaaca tgtatacgta tatatccata aacacgtata cgaaatttag ttaagctata
     3241 cctaaccgct tattccaata aaaaaagtga tcagctttaa actgatcact caccaaaaac
     3301 ttcatcatac aagttatcta tcatttcacc aagtaaagct gaatctgatc gaattcctaa
     3361 cttttcgctg attttagata gtttctttct ttgtgatggt tgaagcgtga attgataatt
     3421 tttcttcttt tcagaactat cactttttag tttagctacc aaagaacttg ttgtttcttt
     3481 aggtgtttgt ttaggttctt caactgcttc aaccacttca ggaacttctt ctacttcagc
     3541 ttcagcgaat tttttaaatc tattttcttt cacttcaatc actccttatt cttgtgccgt
     3601 atcaattatt ttgttgtata gcgtaaacgt gctttcaaag aaatccttat tcttacgata
     3661 gatattctta tccttagcga actcagcaat attcttacct tctaagctag ttgcattaag
     3721 caattccttc tcttgtacat atccaagata attagataac gttggtacca attctaggaa
     3781 ctcacgactt gaattagtat tatgtttgat cctgctaccg atcgtagcgt atttagtttc
     3841 catcagtgtt tcaccagtct taatatcaat caactgtttt gctagtgcat caatatcttt
     3901 accaagctta agcaaagctt caaaaccatc tttagatgga tcagaaatgc ctacgacaag
     3961 atcagataca gcccaagcat tttgagctag tagacctgca tcattatgag tatcaatcag
     4021 aattagatca tacttctctt taagctgttc atagttaaca acgatccatc taaacagtag
     4081 ggttctgttg ttaggcatat cacgcatttg catctcaact tgttttaaat cttcacctgc
     4141 tactaaaata tccatgttct catttatagc taaaggttca gctgatccat gaagatataa
     4201 atgattgatt gtattctctc ttttcgcttt actgaaagca tcaggaaatc gttttgatag
     4261 gttttgcgac ttatcttcat caatatacag caccttcata cctaaatttt cgacagcata
     4321 tccacctaga ttgtagattg ttgttgtttt acctgttcca cctttttcaa tgtttccagt
     4381 aatgattgac aacttctttt ccatgattaa acctccctta ttaatctttt gtaactccat
     4441 tatacactat aaacacgttt atgtatatat ttatatacat aaacgtgttt atatttatat
     4501 ttgcttttat attctacaac ataaacatat atatgtatat gtttatgttt atgtatatat
     4561 tcaaatatat aactattgca tttgtcatac aatcgtagta caatgtaatt gaaaaggagt
     4621 tgataaacta tgccagtaat aagtattcgt gtatctaaag aagaaaaaga ttttcttgaa
     4681 aaagttgctg aattcaacgg agataacatt tctgaattta tgcgtagtaa ttccctgcaa
     4741 acagcagaaa cgttagtaga ctttgccacc tacaagcaac taatgaaaga gcatgaggaa
     4801 aacgatcaaa gtatttccca cgaagatatg ctaaaagagt taggcttata agatgactta
     4861 ccaagtaaga tatgaacgtg aagctcaaaa agcgctaaaa aaaatggata agtttcaggc
     4921 aaaggttatc ctaaactgga tcgagaaaaa tcttgtaggc actgatgatc caagaaagca
     4981 tggtaaaggt ctaacagcta ataaatcagg ctactggcga tatagggtag gtgcataccg
     5041 tataatagct gatattagcg agcatgaagt gacaattctt atattgtcta ttggtcacag
     5101 aagtgaagtt tataagtaac agacaagctg ataaggcttg tctgtttttt tgttaaccca
     5161 acaaaatcac tgtttacgat cccactttac ctaaaaaaga acaatatcct cttgatatta
     5221 tacacaccag tgtgtataat ataagtgtaa acaagctaat taactttcaa aaggagcgat
     5281 tttttatggg atggtatggt gattattcat cagcaaatga agtgatcgaa gaaaagaaag
     5341 ggtacgccaa aaatggtttg gaaatcgtag gtaccaaagt aacgaagacc tatggaatta
     5401 tccttttcaa agatgcagaa ggaacctacg atattgacag cttcatcttt cgagatgaaa
     5461 tgtacaaacc aatctacttc ttaaatgaca gtcctgctta ctttaagaaa attccgaaaa
     5521 aatggttgaa attctttgtc ggtgatcaag aaaaaatggt taagctacaa ctagatagaa
     5581 ttaaagaaga aagcaataaa ccaaaacttg atgacttatt agttgcaggt aaaacttact
     5641 caatctggga tggatcgtat gtcgctgtct atcagtataa actcaaacgt agctttattt
     5701 ttcgactaca agatggatca ctcactcggt tcactcgact atcagcagga gatgttaagg
     5761 aagtaaccta aaacttaaat acataaacat aaacgtgtat acgttta
//
LOCUS       DACGLE010000113         5678 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0534
            SAMEA3304449-rid9560453.denovo.119, whole genome shotgun sequence.
ACCESSION   DACGLE010000113 DACGLE010000000
VERSION     DACGLE010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304449
            Sequence Read Archive: ERR985064
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5678)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5678)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702225.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702225.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 20:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,045
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 27 of 67
            Pseudo Genes (incomplete)         :: 25 of 67
            Pseudo Genes (internal stop)      :: 19 of 67
            Pseudo Genes (multiple problems)  :: 4 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5678
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304449-rid9560453.denovo.119"
                     /strain="VREN0534"
                     /db_xref="taxon:1352"
     gene            133..1287
                     /locus_tag="IXT20_002629"
     CDS             133..1287
                     /locus_tag="IXT20_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HAQ4861037.1"
                     /translation="MEYSEERQRLAYVRLYGGILHLRDSVRISEKEKIKITEMYTSIN
                     GELCKIDKAYSGEIVILQNEFLKLNSVLGDTKLLPQRERIENPLPLLQTTVEPSKPQQ
                     REMLLDALLEISDSDPLLQYYVDSTTHEIILSFLGKVQMEVISALLQEKYHVEIELKE
                     PTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVSPLPLGSGMQYESSVSLGYLNQSF
                     QNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYSPVSTPADFRMLAPIVLEQVLKKA
                     GTELLEPYLSFKIYAPQEYLSRAYNDAPKYCANIVDTQLKNNEVILSGEIPARCIQEY
                     RSDLTFFTNGRSVCLTELKGYHVTTGEPVCQPRRPNSRIDKVRYMFNKIT"
     gene            1385..2758
                     /gene="tet(L)"
                     /locus_tag="IXT20_002630"
     CDS             1385..2758
                     /gene="tet(L)"
                     /locus_tag="IXT20_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002387933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(L)"
                     /protein_id="HAQ4861038.1"
                     /translation="MKCNECNRVKYNQILIWLCVLSFFSVLNEMVLNVSLPDIANDFN
                     KPPASTNWVNTAFMLTFSIGTAVYGKLSDQLGIKRLLLFGIIINCFGSVIGFVGHSFF
                     SLLIMARFIQGAGAAAFPALVMVVVARYIPKENRGKAFGLIGSIVAMGEGVGPAIGGM
                     IAHYIHWSYLLLIPMITIITVPFLMKLLKKEVRIKGHFDIKGIILMSVGIVFFMLFTT
                     SYSISFLIVSVLSFLIFVKHIRKVTDPFVDPGLGKNIPFMIGVLCGGIIFGTVAGFVS
                     MVPYMMKDVHQLSTAEIGSVIIFPGTMSVIIFGYIGGILVDRRGPLYVLNIGVTFLSV
                     SFLTASFLLETTSWFMTIIIVFVLGGLSFTKTVISTIVSSSLKQQEAGAGMSLLNFTS
                     FLSEGTGIAIVGGLLSIPLLDQRLLPMEVDQSTYLYSNLLLLFSGIIVISWLVTLNVY
                     KHSQRDF"
     gene            3322..4584
                     /locus_tag="IXT20_002631"
     CDS             3322..4584
                     /locus_tag="IXT20_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001619005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Plasmid recombination enzyme type 2"
                     /protein_id="HAQ4861039.1"
                     /translation="MSYAVCRMQKVKSAGLKGMQFHNQRERKSRTNDDIDHERTRENY
                     DLKNDKNIDYNERVKEIIESQKTGTRKTRKDAVLVNELLVTSDRDFFEQLDPGEQKRF
                     FEESYKLFSERYGKQNIAYATVHNDEQTPHMHLGVVPMRDGKLQGKNVFNRQELLWLQ
                     DKFPEHMKKQGFELKRGERGSDRKHIETAKFKKQTLEKEIDFLEKNLAVKKDEWTAYS
                     DKVKSDLEVPAKRHMKSVEVPTGEKSMFGLGKEIMKTEKKPTKNVVISERDYKNLVTA
                     ARDNDRLKQHVRNLMSTDMAREYKKLSKEHGQVKEKYSGLVERFNENVNDYNELLEEN
                     KSLKSKISDLKRDVSLIYESTKEFLKERTDGLKAFKNVFKGFVDKVKDKTAQFQEKHD
                     LEPKKNEFELTHNREVKKERSRDQGMSL"
     gene            4829..>5678
                     /locus_tag="IXT20_002632"
     CDS             4829..>5678
                     /locus_tag="IXT20_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000336291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="HAQ4861040.1"
                     /translation="MCPNSSIYSDEKSRVLVDKTKSGKVRPWREKKIANVDYFELLHI
                     LEFKKAERVKDCAEILEYKQNRETGERKLYRVWFCKSRLCPMCNWRRAMKHGIQSQKV
                     VAEVIKQKPTVRWLFLTLTVKNVYDGEELNKSLSDMAQRFRRMMQYKKINKNLVGFMR
                     ATEVTINNKDNSYNQHMHVLVCVEQTYFKNTENYVNQKQWIQFWKKAMKLDYDPNVKV
                     QMIRPKNKYKSDIQSAIDETAKYPVKDTDFMTDDEEKNLKRLSDLEEGLHRKRLISYG
                     GLLKEIH"
BASE COUNT         1952 a          804 c         1167 g         1755 t
ORIGIN      
        1 tccttgtacc ctgtttatca tggaagcgca aaaagcaaca tagggattga gcagcttata
       61 gaagtgataa cgaataaatt ttattcatca acatacagaa agaagtctga actttgcgga
      121 aatgtcttca aaattgaata ttcggaagaa agacaacgtc ttgcatatgt acgcctttat
      181 ggcggaatcc tgcatttgcg ggattcggtt agaatatcgg aaaaggaaaa aataaaaatt
      241 acagaaatgt atacttcaat aaatggtgaa ttatgtaaaa ttgataaggc ttattccggg
      301 gaaattgtta ttttgcaaaa tgagtttttg aagctaaata gtgttcttgg agatacaaag
      361 ctattgccac agagagagag aattgaaaat ccgctccctc tgctgcaaac aactgttgaa
      421 ccgagcaaac ctcaacaaag ggaaatgtta cttgatgcac ttttagaaat ctccgacagt
      481 gacccgcttc tacaatatta tgtggattct acgacacatg aaattatact ttctttctta
      541 gggaaagtac aaatggaagt gattagtgca ctgttgcaag aaaagtatca tgtggagata
      601 gaactaaaag agcctacagt catttatatg gagagaccgt taaaaaatgc agaatatacc
      661 attcacatcg aagtgccgcc aaatcctttc tgggcttcca ttggtttatc tgtatcaccg
      721 cttccgttgg gaagtggaat gcagtatgag agctcggttt ctcttggata cttaaatcaa
      781 tcatttcaaa atgcagttat ggaagggata cgctatggtt gcgaacaagg attatatggt
      841 tggaatgtga cggattgtaa aatctgtttt aagtatggct tatactatag ccctgttagt
      901 accccagcag attttcggat gcttgctcct attgtattgg aacaagtctt aaaaaaagct
      961 ggaacagaat tgttagagcc atatcttagt tttaaaattt atgcgccaca ggaatatctt
     1021 tcacgagcat acaacgatgc tcctaaatat tgtgcgaaca tcgtagacac tcaattgaaa
     1081 aataatgagg tcattcttag tggagaaatc cctgctcggt gtattcaaga atatcgtagt
     1141 gatttaactt tctttacaaa tggacgtagt gtttgtttaa cagagttaaa agggtaccat
     1201 gttactaccg gtgaacctgt ttgccagccc cgtcgtccaa atagtcggat agataaagta
     1261 cgatatatgt tcaataaaat aacttagtgt attttatgtt gttatataaa tatggtttct
     1321 tgttaaataa gatgaaatat tttttaataa agatttgaat taaagtgtaa aggaggaatt
     1381 aattatgaag tgtaatgaat gtaacagggt taaatacaac caaattttaa tttggctttg
     1441 cgttttatct ttttttagcg tattaaatga aatggttttg aacgtctcat tacctgatat
     1501 tgcaaatgat tttaataaac cacctgcgag tacaaactgg gtgaacacag cctttatgtt
     1561 aaccttttcc attggaacag ctgtatatgg aaagctatct gatcaattag gcatcaaaag
     1621 gttactccta tttggaatta taataaattg tttcgggtcg gtaattgggt ttgttggcca
     1681 ttctttcttt tccttactta ttatggctcg ttttattcaa ggggctggtg cagctgcatt
     1741 tccagcactc gtaatggttg tagttgcgcg ctatattcca aaggaaaata ggggtaaagc
     1801 atttggtctt attggatcga tagtagccat gggagaagga gtcggtccag cgattggtgg
     1861 aatgatagcc cattatattc attggtccta tcttctactc attcctatga taacaattat
     1921 cactgttccg tttcttatga aattattaaa gaaagaagta aggataaaag gtcattttga
     1981 tatcaaagga attatactaa tgtctgtagg cattgtattt tttatgttgt ttacaacatc
     2041 atatagcatt tcttttctta tcgttagcgt gctgtcattc ctgatatttg taaaacatat
     2101 caggaaagta acagatcctt ttgttgatcc cggattaggg aaaaatatac cttttatgat
     2161 tggagttctt tgtgggggaa ttatatttgg aacagtagca gggtttgtct ctatggttcc
     2221 ttatatgatg aaagatgttc accagctaag tactgccgaa atcggaagtg taattatttt
     2281 ccctggaaca atgagtgtca ttattttcgg ctacattggt gggatacttg ttgatagaag
     2341 aggtccttta tacgtgttaa acatcggagt tacatttctt tctgttagct ttttaactgc
     2401 ttcctttctt ttagaaacaa catcatggtt catgacaatt ataatcgtat ttgttttagg
     2461 tgggctttcg ttcaccaaaa cagttatatc aacaattgtt tcaagtagct tgaaacagca
     2521 ggaagctggt gctggaatga gtttgcttaa ctttaccagc tttttatcag agggaacagg
     2581 tattgcaatt gtaggtggtt tattatccat acccttactt gatcaaaggt tgttacctat
     2641 ggaagttgat cagtcaactt atctgtatag taatttgtta ttactttttt caggaatcat
     2701 tgtcattagt tggctggtta ccttgaatgt atataaacat tctcaaaggg atttctaaat
     2761 cgttaaggga tcaactttgg gagagagttc aaaattgatc ctttttttat aacaggaatt
     2821 caaatctttt tgttccatta aagggcgcga ttgctgaata aaagatacga gagacctctc
     2881 ttgtatcttt tttattttga gtggttttgt ccgttacact agaaaaccga aagacaataa
     2941 aaattttatt cttgctgagt ctggctttcg gtaagctaga caaaacggac aaaataaaaa
     3001 ttggcaaggg tttaaaggtg gagatttttt gagtgatctt ctcaaaaaat actacctgtc
     3061 ccttgctgat ttttaaacga gcacgagagc aaaacccccc tttgctgagg tggcagaggg
     3121 caggtttttt tgtttctttt ttctcgtaaa aaaaagaaag gtcttaaagg ttttatggtt
     3181 ttggtcggca ctgccgacag cctcgcagag cacacacttt atgaatataa agtatagtgt
     3241 gttatacttt acttggaagt ggttgccgga aagagcgaaa atgcctcaca tttgtgccac
     3301 ctaaaaagga gcgatttaca tatgagttat gcagtttgta gaatgcaaaa agtgaaatca
     3361 gctggactaa aaggcatgca atttcataat caaagagagc gaaaaagtag aacgaatgat
     3421 gatattgacc atgagcgaac acgtgaaaat tatgatttga aaaatgataa aaatattgat
     3481 tacaacgaac gtgtcaaaga aattattgaa tcacaaaaaa caggtacaag aaaaacgagg
     3541 aaagatgctg ttcttgtaaa tgagttgcta gtaacatctg accgagattt ttttgagcaa
     3601 ctggatccag gagaacaaaa acgatttttt gaggaaagtt ataaattatt ttccgaacga
     3661 tatggcaagc aaaatattgc ttatgcaaca gttcataatg atgagcaaac ccctcacatg
     3721 catttaggtg ttgtgcctat gcgtgatgga aaactgcaag gaaaaaatgt gtttaatcgt
     3781 caagaactgt tatggctaca agataaattc cccgagcata tgaaaaaaca gggttttgag
     3841 ttgaagcgtg gtgaacgtgg ctctgaccgt aaacatattg agacagctaa atttaaaaaa
     3901 caaactttgg aaaaagagat tgattttcta gaaaaaaatt tagcagttaa aaaagatgaa
     3961 tggactgctt atagcgataa agttaaatca gatttagaag taccagcgaa acgacacatg
     4021 aaaagtgttg aagtgccaac gggtgaaaag tccatgtttg gtttgggaaa agaaataatg
     4081 aaaacagaaa agaaaccaac caaaaatgtt gttatatcgg agcgtgatta taaaaactta
     4141 gtgactgctg cgagagataa cgataggtta aaacagcatg ttagaaatct catgagtact
     4201 gatatggcga gagaatataa aaaattaagt aaagaacatg ggcaagttaa agaaaaatat
     4261 agtggtcttg tagagcgatt taatgaaaat gtaaatgatt ataatgagtt gcttgaagaa
     4321 aacaagtctt taaagtctaa aataagcgat ttaaagcgtg atgtgagttt aatctatgaa
     4381 agcactaagg aattccttaa ggaacgtaca gacggcttaa aagcctttaa aaacgttttt
     4441 aaggggtttg tagacaaggt aaaggataaa acagcacaat tccaagaaaa acacgattta
     4501 gaacctaaaa agaacgaatt tgaactaact cataaccgag aggtaaaaaa agaacgaagt
     4561 cgagatcagg gaatgagttt ataaaataaa aaaagcacct gaaaaggtgt ctttttttga
     4621 tggttttgaa cttgttcttt cttatcttga tatatataga aataacgtca tttttatttt
     4681 agttgctgaa aggtgcgttg aagtgttggt atgtatgtgt tttaaagtat tgaaaaccct
     4741 taaaattggt tgcacagaaa aaccccatct gttaaagtta taagtgacca aacaaataac
     4801 taaatagatg ggggtttctt ttaatattat gtgtcctaat agtagcattt attcagatga
     4861 aaaatcaagg gttttagtgg acaagacaaa aagtggaaaa gtgagaccat ggagagaaaa
     4921 gaaaatcgct aatgttgatt actttgaact tctgcatatt cttgaattta aaaaggctga
     4981 aagagtaaaa gattgtgctg aaatattaga gtataaacaa aatcgtgaaa caggcgaaag
     5041 aaagttgtat cgagtgtggt tttgtaaatc caggctttgt ccaatgtgca actggaggag
     5101 agcaatgaaa catggcattc agtcacaaaa ggttgttgct gaagttatta aacaaaagcc
     5161 aacagttcgt tggttgtttc tcacattaac agttaaaaat gtttatgatg gcgaagaatt
     5221 aaataagagt ttgtcagata tggctcaaag atttcgccga atgatgcaat ataaaaaaat
     5281 taataaaaat cttgttggtt ttatgcgtgc aacggaagtg acaataaata ataaagataa
     5341 ttcttataat cagcacatgc atgtattggt atgtgtggaa caaacttatt ttaagaatac
     5401 agaaaactac gtgaatcaaa aacaatggat tcaattttgg aaaaaggcaa tgaaattaga
     5461 ctatgatcca aatgtaaaag ttcaaatgat tcgaccgaaa aataaatata aatcggatat
     5521 acaatcggca attgacgaaa ctgcaaaata tcctgtaaag gatacggatt ttatgaccga
     5581 tgatgaagaa aagaatttga aacgtttgtc tgatttggag gaaggtttac accgtaaaag
     5641 gttaatctcc tatggtggtt tgttaaaaga aatacata
//
LOCUS       DACGLE010000114         5652 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0534
            SAMEA3304449-rid9560453.denovo.120, whole genome shotgun sequence.
ACCESSION   DACGLE010000114 DACGLE010000000
VERSION     DACGLE010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304449
            Sequence Read Archive: ERR985064
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5652)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5652)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702225.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702225.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 20:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,045
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 27 of 67
            Pseudo Genes (incomplete)         :: 25 of 67
            Pseudo Genes (internal stop)      :: 19 of 67
            Pseudo Genes (multiple problems)  :: 4 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5652
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304449-rid9560453.denovo.120"
                     /strain="VREN0534"
                     /db_xref="taxon:1352"
     gene            complement(92..763)
                     /locus_tag="IXT20_002633"
     CDS             complement(92..763)
                     /locus_tag="IXT20_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4861041.1"
                     /translation="MIKEIKKVQIALLAFGVFVVCYNFYEFITQKYSTSQGITFIVES
                     LLGIALIFMPQVILKVFKLKIPAAIVLFYWFFLFISVFLGTGMHLISIISFWDKILHA
                     VSPMVLTALGYGLIGYLMKDAEISKTSPWLFLLFGFAFAGLCGVFWEFWEFLCDQFLG
                     MNLQRFAASDGTLFVGRAALMDTMGDLLTNTIGAALMGLFAWSQSKKDERYFESYKLE
                     KVKNT"
     gene            complement(756..1250)
                     /locus_tag="IXT20_002634"
     CDS             complement(756..1250)
                     /locus_tag="IXT20_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deoxyribosyltransferase"
                     /protein_id="HAQ4861042.1"
                     /translation="MKIYFAAPMFAKSDLLYNQQLVKEIRELSSELSIYLPQENEAIN
                     DKTAYADSRMIALADTEKVLASDLMIALLDGLTIDAGVASEIGVAYAKGIPVIGLYTD
                     TRQQGGTHPKKIAALQETAENQFHYLNLYTIGLIKLNGKVVSSEIELLSEVKRFLDGG
                     TFSD"
     gene            complement(1270..2490)
                     /locus_tag="IXT20_002635"
     CDS             complement(1270..2490)
                     /locus_tag="IXT20_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ4861043.1"
                     /translation="MDQKDFRENLELKPVTQEHIDQFNELLSYVFQVTEADIEESGFE
                     NKRAFIRSKKPILEVSKVFGWFHENKLISQIAIYPCEVNIHGARYKMGGVTGVGTYPE
                     YANHGLMQDLIIVALDNMRKNKQWISYLYPYSIPYYRRKGWEIMSDKLSFKIRDTQLP
                     KQIPVSGMVERLPVDHPDVFSVYDKFAQQNHGALFRSDFHWEEYWRFENEDERTAAVY
                     YDSNHEPRGVLFYWVAEEIFHVKEMFYLDQEARNGLWNFISAHFSMIYWVHGDIYKNE
                     PLAFLIEDSQIKEQIEPYFMARIVDVKEFLQRFPFVGTADAFHFIIEDPVAPWNNGVF
                     ALTWDEQGQVRVLNEPIGKSVRLDIQTLTCLMMNYRRASYLARIERLETDEETLKSLE
                     RIIPNMEAYFSDYF"
     gene            2637..4472
                     /gene="lepA"
                     /locus_tag="IXT20_002636"
     CDS             2637..4472
                     /gene="lepA"
                     /locus_tag="IXT20_002636"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HAQ4861044.1"
                     /translation="MDIKKMKERQEQIRNFSIIAHIDHGKSTLADRILEMTHTVTSRE
                     MQDQLLDSMDLERERGITIKLNAVELNYTAKNGETYIFHLIDTPGHVDFTYEVSRSLA
                     ACEGAVLVVDAAQGIEAQTLANVYLALDNDLEILPVINKIDLPAADPERVRQEIEDVI
                     GIDASEAVLASAKSGIGIEDILEQIVEYVPAPSGDLDAPLKALIFDSIYDSYRGVVLN
                     VRITDGMVKPGDKIKLMSNGKTFDVTEVGVFSPKAVARDFLMVGDVGYITASIKTVQD
                     TRVGDTVTLADNPAAEALPGYRKMNPMVYCGLYPIDTSRYNDLREALEKLQLNDAALQ
                     FEPETSQALGFGFRCGFLGLLHMDVVQERLEREFNLELITTAPSVIYHVNKTDGTTAT
                     VDNPADFPEPVTIQDVEEPFVKAQIMVPNDFVGAVMELSQRKRGEFITMDYLDDYRVN
                     VVYNIPLSEIVFDFFDKLKSSTKGYASLDYEMSGYQKSKLVKMDILLNGEKVDALSFI
                     VHRDFAYERGKAIVEKLKKLIPRQQFEVPIQAAIGQKIVARSDIKALRKNVLAKCYGG
                     DVSRKRKLLEKQKEGKKRMKQIGSVEVPQEAFMAVLKMDEDEPKK"
     gene            complement(4566..5648)
                     /locus_tag="IXT20_002637"
     CDS             complement(4566..5648)
                     /locus_tag="IXT20_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017414133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAQ4861045.1"
                     /translation="MNDDGSRKKVEKVGGDTRPEAEAALRKVLSDIDETGQYFLGTDT
                     RVKQYLDFWMEEYVKLNLKYNTYENYRFTIKNHINGYLGKKKLTDLSPALLQNFINAE
                     FKKGYSKKTMTITHSVLKNALNMAVYPWGLIKQNPMLYVKIPKYEARPTTKKDLKIIS
                     LEDFDHMLEITPEGHPFYIPLNIGFYTGMRVGEVCGLTWDDVDFSNGTITVEKQMVKN
                     DGAWVYGTPKTSSSNRTIFIGQTLLAILKKHKKQQLENRMKYGKLYIDSNAVCTKEDG
                     ELVTPSVVKWNTRRISNALSLSFNFHSLRHTHATLLLENGAKMKEISERLGHSRISIT
                     MDTYSHVTDKMRNETVDIMENLRKNS"
BASE COUNT         1725 a         1135 c         1076 g         1716 t
ORIGIN      
        1 tttttcaaat aaacagtgtt ccagaagtag tttcttcgaa aaataagccg aaatttcttg
       61 aagctgcccg cttctgtcac aacctctttt attaggtatt tttcactttt tctaatttat
      121 aactttcaaa gtaacgctca tcttttttac tttgagacca tgcaaacaat cccattaagg
      181 cagcacctat cgtatttgtc agcaagtcgc ccatcgtatc catcaatgct gctcgtccga
      241 caaacagtgt gccatcagaa gcagcaaaac gttgaagatt catgcctaag aactggtcgc
      301 ataagaattc ccagaactcc caaaacacgc cgcaaagtcc agcaaacgca aagccgaata
      361 agaggaacaa ccaaggactg gtttttgata tctcagcatc tttcatcaag taaccaatca
      421 atccatagcc aagagctgtc aggaccattg gactcaccgc atgtaagatt ttgtcccaaa
      481 aagagatgat actgatcaaa tgcatccctg tgcctaaaaa gacagagatg aacaagaaaa
      541 accagtaaaa caaaacgatt gctgctggta ttttaagttt gaaaactttc aaaatgactt
      601 gtggcatgaa aatcaatgca atccctaata aagattccac gataaatgtg attccttgag
      661 aagtcgaata tttttgtgtg ataaattcat aaaaattata gcagacaacg aatacaccaa
      721 aagcaagtaa agctatttgc acctttttga tttctttaat cactgaaagt ccctccatct
      781 aaaaatcttt ttacttctga aagcaactca atttctgaag aaaccacttt tccgttcaat
      841 ttgatcaaac ctattgtata taagttcagg tagtgaaatt gattttcagc tgtttcttgt
      901 agtgcagcga tttttttcgg gtgggttccg ccttgctgtc gagtatcagt atataagccg
      961 atcactggga tgcctttcgc ataagccaca ccgatttccg atgctacccc ggcatcgatc
     1021 gtcagcccat ctagtaaagc aatcattaaa tcactagcca acactttttc tgtgtcagca
     1081 agagcaatca ttcgactgtc tgcataagca gttttgtcat tgattgcttc attttcttga
     1141 ggaagataga tcgaaagctc agaagagagc tctctgattt ctttaactaa ctgttggtta
     1201 tataacaaat cacttttagc aaacattggt gcggcaaaat aaattttcat ctaaacatca
     1261 tcctttttat caaaaataat cgctaaagta agcttccatg tttggaatga tacgctctag
     1321 tgacttcagt gtttcttcat ctgtttctaa ccgttcgatt cttgccaaat aagaagcacg
     1381 gcgataattc atcatcaagc aagtcaatgt ttggatatcc agtcgaacgg attttcctat
     1441 aggttcattt aagacacgga cttgtccctg ttcatcccat gttaaagcga agacaccatt
     1501 attccaagga gcgacaggat cttcaatgat gaaatgaaaa gcatcagctg ttccaacaaa
     1561 cggaaaccgt tgaaggaatt cttttacatc aacgatccga gccataaaat atggttcgat
     1621 ctgctctttg atctgactat cttcaatcaa aaatgctaaa ggctcatttt tataaatatc
     1681 tccatgtacc cagtagatca tggaaaaatg tgcggaaata aagttccata agccatttcg
     1741 agcttcttga tcaagataga acatttcttt tacgtgaaat atttcttcag ctacccagta
     1801 gaacagaaca cctcttggtt catggttgct atcgtagtag acagcggctg tgcgctcatc
     1861 ttcattttcg aaacgccaat actcttccca atgaaaatca ctgcggaata aagcaccatg
     1921 gttttgttgg gcaaatttgt cataaacgga aaatacgtct ggatgatcaa ctggtaatcg
     1981 ctcaaccata ccacttactg ggatttgttt aggtagctgt gtatctctga ttttaaagga
     2041 aagtttatca gacatgattt cccatccttt acgccgatag taggggatac tgtaaggata
     2101 caaataagag atccattgct tattcttgcg catattatcc aatgcaacga taattagatc
     2161 ttgcatcaat ccatggttag catattctgg ataagttcca actccagtca caccacccat
     2221 tttataacgt gcaccgtgaa tattgacttc acaaggatag atagcgattt gagagatcag
     2281 tttattttcg tggaaccatc cgaacacttt cgatacttcc aaaatcggtt ttttggaacg
     2341 gataaaagct cgtttattct caaacccgct ttcctcgatg tctgcttcag tcacttggaa
     2401 cacataggaa agcaattcat tgaattgatc gatatgttct tgagtgaccg gttttaattc
     2461 aagattttct ctaaaatctt tttgatccat tttttcatct ccatttatta gcttgataac
     2521 agtatagcaa aattctacaa aaaaaacttc tatagctgac taattaaaaa ctctttaaat
     2581 tactgctttt cgttggtata atggaaagtg ctgattatat gaaaagagga gacgctatgg
     2641 atataaagaa aatgaaagaa cgccaagagc agattcgtaa tttttcgatc atcgctcata
     2701 tcgaccatgg gaaatctaca ttggccgatc gtattttaga aatgacgcac actgtgacgt
     2761 ctcgcgaaat gcaagatcag ctgttagact cgatggattt ggaacgtgaa cgtggaatca
     2821 cgattaaatt aaatgctgta gaactcaatt acacagcgaa aaatggagaa acatatatct
     2881 ttcacttgat tgacacccca gggcacgtcg acttcaccta tgaagtctct cgaagccttg
     2941 ctgcctgtga gggtgccgtt ttagtggtcg atgcagcaca aggaatcgaa gcacaaacat
     3001 tggccaatgt ttatttagcg ttagacaatg atttggagat cctgccagtc atcaataaaa
     3061 tcgacctacc cgcagcagat cccgaacgtg tccgtcaaga aatcgaagac gtaatcggaa
     3121 tcgatgctag tgaagctgta ttagccagtg ccaaatcagg gattgggatt gaagatattc
     3181 tcgagcagat cgtggaatat gttcctgcac caagtggaga cttagatgca ccgctaaaag
     3241 ctttgatctt tgactctatt tatgatagtt accgcggtgt tgttctaaat gtccggatca
     3301 cggacggtat ggtcaaaccc ggagacaaaa tcaaactgat gagtaatggc aaaacatttg
     3361 atgttacaga ggtcggggtc ttctcgccaa aagcagttgc tagagacttt ttgatggtcg
     3421 gtgatgtcgg ttatatcaca gcaagtatca aaactgtcca agatacacga gtaggggata
     3481 cagtaacgtt agcagacaat cctgccgctg aagctttacc aggttaccga aaaatgaatc
     3541 cgatggttta ttgtggtctt tatccaattg atacttctcg ttacaacgat ttgcgggaag
     3601 ctttagaaaa actgcaattg aatgatgctg ctttacaatt cgaaccagaa acctctcaag
     3661 cattaggttt tgggttccgc tgtggattcc tcggacttct tcatatggat gtcgtccaag
     3721 aacgcttgga acgggaattc aatctagaat tgatcacgac tgcaccatcc gttatttacc
     3781 atgttaataa aacggatggt acgactgcta cagtagataa cccggcagat tttcctgaac
     3841 ctgtaacgat tcaggatgta gaagaaccat tcgtcaaagc acagatcatg gtcccaaatg
     3901 attttgtcgg tgcagtaatg gaactttctc aacgaaaacg gggagaattc attacgatgg
     3961 attatttaga tgattaccga gtaaatgtcg tctataatat tccgctttct gaaattgtct
     4021 ttgacttttt cgacaaattg aaatcaagca cgaaaggcta tgcctcattg gattatgaaa
     4081 tgtctggtta tcaaaaaagc aagttagtga agatggacat cttgctgaat ggagaaaaag
     4141 tcgatgcatt aagctttatc gttcaccgag actttgccta tgaacgtgga aaagcaatcg
     4201 ttgaaaaact gaaaaaacta atcccacgcc aacaatttga ggtgccaatc caagcagcta
     4261 ttggacaaaa aatcgttgcc cgttcagaca tcaaagcctt gcgtaaaaac gtcttggcta
     4321 aatgttatgg tggagatgtt tctcgtaaac gtaaactctt agagaaacaa aaagaaggga
     4381 agaagcggat gaaacaaatc ggctccgtcg aagttccgca agaagccttc atggccgttc
     4441 tgaaaatgga cgaggacgaa ccgaagaaat agcaagggtt tgtttgaaaa cagtgtaaaa
     4501 aataaaccat gtttacatgg atttgccacc ggtttgccac cggtggtttt tttcggtggc
     4561 aaaaatcaag aattctttct aagattttcc atgatatcga ccgtttcatt tctcatctta
     4621 tctgttacat gcgagtaagt atccatcgta attgaaattc tgctgtggcc caatcgttca
     4681 gagatttctt tcatttttgc gccattttcg agaagaagtg tagcatgagt atgtctgaga
     4741 gaatggaagt taaaagagag ggagagtgca ttcgatatcc tcctagtatt ccatttcact
     4801 acacttggcg taactagctc accgtcttcc ttcgtacata ctgcatttga atcaatgtag
     4861 agctttccat acttcattcg attttctaat tgttgtttct tatgtttttt cagaattgct
     4921 agcaaggttt gtccaataaa aatcgttcga ttggaactgc ttgtctttgg cgtaccatat
     4981 acccatgcgc cgtcattctt taccatttgt ttctctacag taattgttcc atttgaaaaa
     5041 tcgacatcat cccacgtcag accacaaact tcgccaacgc gcattcccgt ataaaatcca
     5101 atattcaaag gaatatagaa aggatgtcct tcaggagtaa tttctagcat atgatcaaag
     5161 tcctcaagag aaatgatttt tagatctttt ttagtcgttg gtcgtgcttc atattttggt
     5221 atttttacat acagcatagg attttgcttg attaatcccc aaggataaac cgccatattc
     5281 agcgcattct taaggacaga gtgagtaata gtcattgttt tcttcgagta acccttttta
     5341 aattcagcat tgatgaaatt ttgtaaaaga gcaggggaga gatccgtaag ttttttcttt
     5401 cctaaataac cgtttatatg atttttgatg gtaaatcggt agttttcata ggtattgtat
     5461 tttagattta gtttaacgta ttcctccatc caaaaatcaa ggtattgttt tactcgagta
     5521 tccgtaccta aaaagtattg tcctgtttcg tcaatatctg ataaaacttt tcgtaaagca
     5581 gcttcggcct ctggtcgggt gtctccgcca actttctcca ctttttttct tgagccatca
     5641 tcattgatat ct
//
LOCUS       DACGLE010000115         5631 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0534
            SAMEA3304449-rid9560453.denovo.121, whole genome shotgun sequence.
ACCESSION   DACGLE010000115 DACGLE010000000
VERSION     DACGLE010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304449
            Sequence Read Archive: ERR985064
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5631)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5631)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702225.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702225.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 20:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,045
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 27 of 67
            Pseudo Genes (incomplete)         :: 25 of 67
            Pseudo Genes (internal stop)      :: 19 of 67
            Pseudo Genes (multiple problems)  :: 4 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5631
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304449-rid9560453.denovo.121"
                     /strain="VREN0534"
                     /db_xref="taxon:1352"
     gene            187..2247
                     /locus_tag="IXT20_002638"
     CDS             187..2247
                     /locus_tag="IXT20_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4861046.1"
                     /translation="MCSKRRSKQLNKHACRLDKERSCSEKHYVKFMMRTMLISLSVGV
                     YMHVPVQATTQTIKVVRTSVSETTDSPITITHTFTDANGKQLLPALTSYNKVSYPPTI
                     NHYWLIPNKTLWTSETGSSVPLSIFSDMTDPTKGDQLKQITDILNHALTDIKDGGSAQ
                     VSYVYEKDYSQFERKKIDVLAGTKVSISDLVERLKNADGTQGDMSKVISNNGNIVDTT
                     NTGTSTISLSYFDPIALKTMTASAVIHVRADQTAIKGKDLHLRTGESYAPKDLVEYVT
                     KSDGTSGNKEEIKMNGTVDTTTPGEYPVVLTYIDSKTSNVAETTAVVYVEASAASSTV
                     ITHFIDSQTNKELQAETHMSSVNRIPDLRLPAEFSDRVPNIDLSTYEQNGKVTSISDW
                     MQANGLDRKSHWSEILEVLHSELMNNRKQSGGEITFTYVYIPDNRSLEGHDLIVHPNE
                     KINKEELIQSATNSLGKPVPLKDVSVTINEVPLEDGYLTKQTGDFTIMYSYHDPYSLQ
                     ELHAQSLLHVLPLNKQIDQKEQKRQEESLSEKKQQMAHAVSPTGKVKQTRSLELARIS
                     RKISESNKTIRQEAHLAPQVSGLENEILEPKIKQQSEKEFVAKQSEDQKESDDQKETK
                     KKRLSTIDSTDEHLQPQVPLPSPGTPAPGGGIPSSALGDFMRGMAGTWVYANRNEFDA
                     DDWF"
     gene            2330..2899
                     /locus_tag="IXT20_002639"
     CDS             2330..2899
                     /locus_tag="IXT20_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ4861047.1"
                     /translation="MLDKTLPYAEIWMTRPLQQNVPITPLAEGFEFRFYQKEDESAWA
                     EIETSVGEFQTTDEALEYFQQNFAPYPEELERRMFFVETKKGEKVATCTAWRKKSREQ
                     VYPVFHWLAVKPDYQGKGLAKALTARVLQEFPTLHTDGPIYLHTQTWSHQAIALYKKM
                     GFTFIAENLDGTPNPDYEKVMQVLSKLDK"
     gene            complement(3074..3880)
                     /locus_tag="IXT20_002640"
     CDS             complement(3074..3880)
                     /locus_tag="IXT20_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4861048.1"
                     /translation="MNHLKTFFTNSTKTAGRITNGIYILLRIIVVIMLVDQVWNQHWG
                     NTMLLLLTLILFEIPYFLEKILKIQIPNMLEIILLSFIFSSTVLGELSDFYGYFSMWD
                     TFLHAFSGFLAGGVGFSLVYLLNKNTKTMNLTPLLLAVVSFCFSMTVGVMWEFVEFSA
                     DKLLQFDMQKDTIIQQINSVELSKDGNTVYHVPDITRTIIESQNADGKTDKTIIQNGY
                     LDVGIMDTMKDLFVNFIGAVVFSVFGYLYSRNDYQKFTFIKNFIPKKIKR"
     gene            4018..5532
                     /locus_tag="IXT20_002641"
     CDS             4018..5532
                     /locus_tag="IXT20_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavocytochrome c"
                     /protein_id="HAQ4861049.1"
                     /translation="MKKLVAGLLTAVALLAMSACGADQKNTAQTSSSTKESAEVSSGA
                     SEQVYTDPKEMKESYDVVIIGAGGAGMAAAIEAKDAGLNPVILEKMPVAGGNTSKSSS
                     GMNASETKFQKEEGIKDSNDAFYEETLKGGGGTNDKEMLRYFVDHSADAVDWLDQNGI
                     KLDNLTITGGMSEKRTHRPSDGSAIGGYLVEGLLNNVHEREIPIFVNADVTDIKKNGE
                     TVNEVTVHVQGEEPKEVTGKAIVVTTGGFGASKEFIEKYRPDLADYITTNQEGSTGDG
                     ITMIEKLGGQTVDMDKIQIHPTVQQDEGVLIGEVVRGEGAILVDQEGKRFVNEMDTRD
                     KVSAAITALPEKSAYLIFDQGVRDRATAIEFYDQKGYVTTGKTMEELAEKIDLPKETL
                     AKTVDGWNTDVAEKKDAQFNRATAMEHDLSSPNYYAIKIAPGIHYTMGGVKINTNTEV
                     LDKENQPIRGLYAAGEVTGGLHGNNRIGGNSVAEIIIFGRQAGIQAARFASENE"
BASE COUNT         2010 a          970 c         1132 g         1519 t
ORIGIN      
        1 tagatatatt acttaacaaa attaaaaagt acataagtta ttattccgat tttcttgaaa
       61 caataaaaaa tcaaaaaaaa tactctccta atagtccatc ttcttttgat tttatttata
      121 taatgaaatc atcttatgaa aattactact tcatgtttag acaagagtag ggaggactaa
      181 gaataaatgt gttcaaaaag acgttcaaaa caactaaata aacacgcttg tcgtttagat
      241 aaggaacggt cgtgttctga aaaacactac gttaagttta tgatgcgcac gatgctgatc
      301 agtttatctg taggtgtcta tatgcatgta cccgttcagg caacgacaca aaccatcaag
      361 gtcgtacgta cctctgtttc tgagaccact gattccccta tcactattac tcatactttc
      421 actgatgcaa atggaaaaca gctacttcct gcattgactt cctataataa agtcagttat
      481 cctccaacga taaaccacta ctggttaatt cctaacaaaa cattatggac atcggaaacc
      541 ggaagcagtg tgccgctttc tatcttttcc gatatgactg atcctacaaa aggtgatcag
      601 ttaaaacaga tcactgatat tttgaatcat gctttgacag atataaagga cggtggaagc
      661 gcacaagttt cttatgtata tgaaaaagat tattcacagt ttgaaagaaa gaaaatcgat
      721 gttctggcag ggacaaaagt ttcgatatct gatctggtag aaaggctgaa gaacgcagat
      781 ggaacacaag gagatatgtc caaagtaatt tcaaacaatg ggaatatcgt ggatacgact
      841 aatacaggaa catctactat ttccttgtcc tattttgatc caattgcttt aaaaacaatg
      901 actgcatctg ctgtgatcca tgttcgtgct gatcaaacag ctatcaaagg aaaagatctt
      961 cacttaagaa caggagagag ttacgcccct aaagatcttg ttgaatatgt aacaaaaagc
     1021 gatggaactt caggaaataa agaagaaatc aaaatgaacg ggacagtaga tactacaact
     1081 ccgggagaat atcccgttgt attaacttat atagactcga aaacatcaaa tgtggcagaa
     1141 acaacagctg tagtttatgt agaagcatct gctgcttcat cgactgtcat cactcatttt
     1201 atagatagtc aaacaaataa agaacttcaa gcagaaacac atatgagtag tgtaaatcgt
     1261 attcctgatt tgcgcctacc tgctgaattt tctgatcgag tgccaaatat agatttatcg
     1321 acttatgaac aaaatgggaa agtcacttct atttctgatt ggatgcaagc aaacggatta
     1381 gacagaaaaa gtcactggtc ggagatcctt gaagtacttc actcagaact gatgaataat
     1441 aggaagcaat caggagggga aattaccttt acttatgttt atataccaga caatcgttca
     1501 ttagaaggtc atgatcttat tgttcatcca aacgaaaaaa taaacaaaga agagttgatc
     1561 caatcagcaa caaattcctt aggtaaaccc gtaccgttaa aggatgtatc ggttacaatc
     1621 aatgaagtac cattggagga tggttatttg accaaacaaa caggtgattt tacgattatg
     1681 tatagttatc atgacccgta ttcactccaa gaactccatg ctcagtcact attacatgtt
     1741 ttaccattaa acaaacagat agatcaaaaa gaacaaaaaa gacaagaaga aagcttgtca
     1801 gaaaaaaaac aacaaatggc tcatgcagtt tctccaacag ggaaagtaaa acaaacaaga
     1861 agcctagagt tagcaagaat atctagaaaa atctcagaat ctaataaaac gataaggcaa
     1921 gaagctcatt tggccccaca agtttctgga ttggaaaatg aaattttaga gcctaaaata
     1981 aaacaacaat cagaaaaaga atttgttgct aaacagtcgg aagatcagaa ggaatcagat
     2041 gaccagaagg aaacgaagaa aaaacgcttg tcaactattg attccaccga tgagcatctg
     2101 cagccgcaag taccacttcc tagtccggga acacccgcac ctggtggagg tattccttct
     2161 tctgctttag gagatttcat gcgaggtatg gcaggaacat gggtatacgc taatcgaaat
     2221 gaatttgacg cagatgactg gttctgattt atatctctat ctgttatagt caaataagta
     2281 gctgagtcat atgttatttg gcataatgga gacgaggagg gatagaaaaa tgttagataa
     2341 aacgttacct tacgcagaaa tctggatgac aagaccactt caacaaaatg ttccaatcac
     2401 gcctttagcg gaaggttttg aatttcgatt ttatcaaaaa gaagatgaat ctgcttgggc
     2461 ggagattgag acttccgttg gggaatttca aacgacagat gaagcacttg agtattttca
     2521 acaaaacttt gctccttatc ctgaagaact tgaaagaagg atgttttttg tagagacaaa
     2581 aaaaggagaa aaagtagcaa cctgtaccgc ttggcgaaaa aaatcaagag agcaagtgta
     2641 tccggttttt cattggttag cagtgaaacc agattaccaa ggaaaagggc tcgcaaaagc
     2701 attgactgcc agagtcttgc aagaatttcc taccctccat acggacggac caatctatct
     2761 tcacacacag acatggagcc atcaggcgat tgctctttat aaaaaaatgg gttttacttt
     2821 tattgctgaa aatctagacg gcacacctaa tccagattat gaaaaagtta tgcaggtttt
     2881 aagtaaatta gataaataac gaaatggtga aataagtcct cagtattggg ataaaaaacg
     2941 aaatagtcat tcctcatatc ttactttttt gagttaatac tatcggataa ctaatggcat
     3001 gccatacatt caaaaaagaa ggggcagtga caaaagtatt gtcactgccc cttctcttgt
     3061 tttttagcat gttttatcgt ttgatttttt taggaataaa attcttgatg aacgtaaatt
     3121 tttggtaatc gtttcgacta tagaggtaac caaatacact gaaaacaact gctccaataa
     3181 aattaacaaa taaatccttc atagtatcca tgattcccac gtctaagtaa ccattttgga
     3241 tgattgtttt atctgtttta ccatctgcat tttgactttc aatgattgtc cgagtgatat
     3301 ctggaacatg atagacagtg tttccgtctt tacttaactc cacactattg atttgctgga
     3361 tgatcgtatc tttttgcata tcgaattgca gtaatttatc tgctgaaaat tccacaaact
     3421 cccacataac accaactgtc atcgaaaaac agaaggaaac aactgctagt aataaaggag
     3481 tcaagttcat ggtttttgta tttttattga gcaaataaac aagggaaaag ccgacacccc
     3541 cagctaaaaa tccgctgaat gcatgtagaa aagtatccca cattgaaaaa tagccataaa
     3601 aatcacttaa ctctcctaaa actgttgaac taaaaataaa agataacaaa atgatttcta
     3661 gcatatttgg aatctgtatc tttaatatct tttccagaaa ataagggatt tcaaataaaa
     3721 ttaatgtcag taataaaagc attgtatttc cccagtgttg gttccagacc tgatccacta
     3781 gcataataac gacgatgata cgtaataaaa tatagattcc gtttgtgatt ctacctgcag
     3841 tttttgtact gttcgtaaaa aaggttttca aatgattcat aaaataactc cttcatgtat
     3901 atccttaatt atactaagaa gcgtgtgttt accaaagcta tatgacttaa agaaaaacag
     3961 agttgatatt gtgaaaaaaa tcacttaata tagtataagg agaaaagggg gatacaaatg
     4021 aaaaaattag ttgctggttt attaacagcc gttgcacttc ttgctatgag cgcatgcggc
     4081 gcagatcaga aaaatacagc gcagacaagt tctagtacga aggaatctgc cgaagtatca
     4141 tcaggcgctt ctgaacaagt atatacagat ccaaaagaaa tgaaagaatc ttatgatgtc
     4201 gtgattattg gagctggtgg tgcagggatg gcagcagcta tcgaagcaaa agatgctggg
     4261 ttgaatccag taattttgga aaagatgcct gttgccggag gaaacacatc taaatcatca
     4321 agcggtatga atgcttcaga aacaaaattt caaaaagaag aaggaatcaa agactcgaat
     4381 gatgccttct atgaagaaac gctaaaaggt ggtggaggaa cgaatgataa agaaatgctt
     4441 cgatactttg tcgatcactc tgctgatgcg gtcgattggc tagatcaaaa cggtatcaaa
     4501 ctggacaact taacgatcac tggagggatg agtgaaaaga ggacacatcg tccgtctgat
     4561 ggctcggcta ttggtggata cttagtggag gggttattga ataacgtcca tgagcgggaa
     4621 atccctattt ttgtcaacgc agatgtgaca gacattaaga aaaatgggga aacggtcaat
     4681 gaagtgactg tacatgttca aggagaagaa ccaaaagagg tcaccggaaa agcaatcgta
     4741 gttaccacag gcggttttgg tgccagcaaa gaatttattg aaaaatatcg tcccgattta
     4801 gctgattata ttacgacaaa tcaagaagga tcaactggag atggtatcac catgattgaa
     4861 aaattaggtg gacaaaccgt tgatatggat aaaatacaaa ttcatccaac tgtgcagcag
     4921 gatgaggggg tcttgattgg tgaggttgtt cgcggagaag gagcaatctt ggtcgatcaa
     4981 gaggggaaaa gatttgtcaa tgaaatggat acaagggata aagtatctgc agcaattaca
     5041 gcattgcctg aaaaatcagc ttatttgatt tttgaccaag gcgtgcgtga tcgtgccaca
     5101 gctattgaat tttatgacca aaaaggatat gtcacaactg gaaagacaat ggaagaatta
     5161 gcagaaaaaa tcgatttacc aaaagaaaca ctagcaaaaa cagttgatgg atggaatacg
     5221 gatgttgcgg agaaaaaaga tgctcaattc aaccgtgcca cagccatgga acatgactta
     5281 tcgtctccga attattatgc gatcaaaatc gcaccaggaa tccattacac gatgggcggt
     5341 gtgaaaatca atacgaacac tgaagtatta gataaagaaa accagccaat tagggggtta
     5401 tatgctgctg gtgaagtcac aggtggctta catggaaaca accggatcgg tggaaattca
     5461 gttgcggaga tcattatttt tgggcgacaa gcgggcatac aagcagctcg atttgcttcg
     5521 gaaaatgaat gaaatagaac gtaaataaaa agaggttgtg aaatcagcgt tttgagctaa
     5581 taccgagggt cagctgattg aacactgttt agtgaagagg ttgtgaaaaa g
//
LOCUS       DACGLE010000116         5466 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0534
            SAMEA3304449-rid9560453.denovo.122, whole genome shotgun sequence.
ACCESSION   DACGLE010000116 DACGLE010000000
VERSION     DACGLE010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304449
            Sequence Read Archive: ERR985064
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5466)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5466)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702225.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702225.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 20:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,045
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 27 of 67
            Pseudo Genes (incomplete)         :: 25 of 67
            Pseudo Genes (internal stop)      :: 19 of 67
            Pseudo Genes (multiple problems)  :: 4 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5466
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304449-rid9560453.denovo.122"
                     /strain="VREN0534"
                     /db_xref="taxon:1352"
     gene            133..1107
                     /locus_tag="IXT20_002642"
     CDS             133..1107
                     /locus_tag="IXT20_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAQ4861050.1"
                     /translation="MRQLHKEQIETAMAALKEKTIDHVYFTACGGSQAVLMAGQYLFD
                     KELTVPCHVYTANEFNYAIPKGLSENSLVITCSHSGTTPETVEATKKAREFGAMTICL
                     SNEEGSPLWEAAEYPVHYDWGKDVDASDLNKGILYGLLFNILLVLDGSKKWETCLNEF
                     DKLNDLVIAEKSKFEITAKEWGKNQKRDKIIYTIGSGINYGEIYSTAACWFMEMQWIN
                     SNAIHSGEFFHGPFEITDYDVPFILVKSIGDTRFLDQRVEDFTKKFSNKLMVLDQADF
                     DLESIDSVAKEYIAAVLSGVVIRQMVEAIAFERGHSLDVRRYMWQMKY"
     gene            1089..1550
                     /locus_tag="IXT20_002643"
     CDS             1089..1550
                     /locus_tag="IXT20_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HAQ4861051.1"
                     /translation="MANEILINRRLQKMANQLFHIVITTHSTLCEGYLKATKLILMND
                     ATGISAVPFKENTSPDDFEESMKEIISQHHLQPLIILTDLIGGTPNNVSIKQLNNNRI
                     QIIAGINLPLLLELLMAQSAGKSIEEIDFKELLNNSQKSMVHINSLLKDVS"
     gene            1600..2064
                     /locus_tag="IXT20_002644"
     CDS             1600..2064
                     /locus_tag="IXT20_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ4861052.1"
                     /translation="MIKLVRVDHRLLHGQVIFSWTKQYDINHIIVADNKVPGDPMSVM
                     ALTIAKPADCKLDIVKISDVKGIMETSPNDNYMILIKGPKEAFELVQNLPEIKEINFG
                     GVSKKSESKQYGKAVFLTPEELEATKKILDQEVKIFVQQVPSSSIENVDFSK"
     gene            2078..2827
                     /locus_tag="IXT20_002645"
     CDS             2078..2827
                     /locus_tag="IXT20_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ4861053.1"
                     /translation="MLLKAILLGLVGVVAVIDSRLIGRQNIGRPLILSTLAGLVLGDL
                     HTGIVLGASLELISMGFVSIGAAGPPNMQLGSIIATAFAILTNSSTETALTIAIPVAV
                     AGEFMSIIMRMIIAQFGHAADKAIENNKFRKAQIIHIYWSFIFNAFVYFIPIFLTVYF
                     GADVVADLVDKIPEMITDALTVSGNMLSALGFAMLLSTMLSKKLYPYFIFGFFIVAYS
                     GLSLIGVTIFAALIAFVMDQVKYGKREEAHG"
     gene            2827..3657
                     /locus_tag="IXT20_002646"
     CDS             2827..3657
                     /locus_tag="IXT20_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAQ4861054.1"
                     /translation="MENSTEKKRLITKKDLHKMCLRSWTIMCSWNYERQMHMGFMYGM
                     APILNKVYKNDEEKKREAYTRHMAFFNCTPQMTPFIMGLCASMEEQNAQAEEGNFNTE
                     SISMIKTSLMGPFAGIGDSFFQGTLRIITFGIGLSFAKQGSILGPILAVGLFAIPSIL
                     AVFYGGKLGYESGNKYLTKLYQEGLMDRVMQFASIIGLAVVGGMVASMVPIVTPLKFT
                     TGGTELVLQDLLDSIIPGLLPLVCTLGIYKLIKKNVSTNMLLLGCILAGFVLSIIGIL
                     "
     gene            3657..4304
                     /locus_tag="IXT20_002647"
     CDS             3657..4304
                     /locus_tag="IXT20_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAQ4861055.1"
                     /translation="MIKALIFDMDGVLIDSEIMYQKWVFNFLKQENLSYPLYSYQKKI
                     GTTASIFDDIEKYNEGCNSLELKGKFTKYWKSQKIDYLDIFPNILIEQLEWLKKKKIK
                     MAIASSSPMDTINEVVTTCKIESYFDCLISGRDLPESKPNPTIFLKASEQLRIPVEEC
                     LVIEDSYNGIKAGKRANMKVLAIKDKRFSQDVSLADDVIYDIKYLRQEVQVKFGI"
     gene            complement(4402..5178)
                     /locus_tag="IXT20_002648"
     CDS             complement(4402..5178)
                     /locus_tag="IXT20_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ4861056.1"
                     /translation="MNFQDNRPLYLQVEASIRNDIREKKYLPGEQLPTEEELCKIYGV
                     SKITIRKAFKLLTESGTVERLRGKGTFVTQKKESLKLAGVHGFSDSLSTRGHKIRYTV
                     LHSKIIKADEFLANKLQINVTDRVYNIKRLMWEDGAPMGIDNFFASEEKFPTLFEKGS
                     QYTSLYKLLEDDYDVKATSSIMEINGLSATPELSDLLQCIVGDPLFILEKVGKDKNNQ
                     PFHYSQSTVRCDRISYVIEVTENITLDTVHKNDSDPKVID"
     gene            5260..5403
                     /locus_tag="IXT20_002649"
                     /pseudo
     CDS             5260..5403
                     /locus_tag="IXT20_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016608918.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
BASE COUNT         1872 a          739 c          973 g         1882 t
ORIGIN      
        1 attttattat ataaaattgt tataaaaaat tttttttgaa aaatagtatt gcaaaataaa
       61 gcaatttcat agataatagg tatcgtaaag ttatataact ttataatacc tattagagtg
      121 agggataaag aaatgagaca actacacaaa gaacagattg aaacagcaat ggcagcgtta
      181 aaggaaaaga caattgatca tgtatatttt acagcatgtg ggggttcaca agccgtttta
      241 atggctggtc aatatctatt tgataaagaa ttaactgtac cttgtcatgt ttatactgct
      301 aacgaattta actatgctat accaaaagga ttatctgaaa attctttagt tatcacttgt
      361 tcccattctg gcacaacacc agaaactgta gaagcaacaa aaaaagcaag agaatttgga
      421 gcaatgacta tttgtttatc taatgaagaa ggatctcctc tatgggaagc agctgaatat
      481 cctgttcact atgattgggg taaagatgtt gatgctagtg atttaaataa aggaattctt
      541 tatggtttat tatttaatat tttacttgtt ttagatggct ctaaaaaatg ggaaacttgc
      601 ttgaatgaat ttgataagtt aaatgatctt gttattgctg aaaagagcaa atttgaaata
      661 actgctaaag aatggggtaa aaaccaaaaa cgagataaaa ttatttacac aattggtagc
      721 ggtattaatt acggagaaat ttactccaca gcagcttgct ggttcatgga aatgcaatgg
      781 attaattcta atgctattca ttctggggaa tttttccatg gtccttttga aatcactgat
      841 tatgatgtac cattcatctt agtgaaaagt attggagata caagattttt agatcaacgg
      901 gtagaagact tcactaaaaa attcagtaat aaattaatgg ttttagacca agctgatttt
      961 gacttagaat ctatagatag tgtagcgaaa gaatatattg cagctgtact tagtggcgtt
     1021 gttattcgcc aaatggtaga agcgattgct tttgaaagag gccattcctt agatgttaga
     1081 cgctatatgt ggcaaatgaa atattaatta ataggaggtt gcaaaagatg gcaaatcaat
     1141 tatttcacat tgtgattaca acacattcta ccctttgtga gggttattta aaggcaacaa
     1201 aattaatttt aatgaatgac gccacaggta ttagtgctgt tccatttaaa gaaaatacta
     1261 gtccagatga ttttgaggag agtatgaaag aaataattag tcagcatcat ttgcaacctc
     1321 taattatttt aacggattta attggaggaa cacctaataa tgtttctatt aaacagttga
     1381 acaacaatag aattcaaatt attgctggga ttaatttacc tcttttactg gagttattaa
     1441 tggcccagtc agctggtaaa tctatagaag aaatagattt taaggaatta ctgaataact
     1501 ctcaaaaaag tatggttcat attaattcgc ttttaaaaga tgtcagttaa ggtggcaatt
     1561 tctattctat aaattttaaa aattgaagga ggaaatagaa tgattaaatt agtgcgtgta
     1621 gaccacagat tacttcatgg acaagtaata ttttcttgga ctaaacagta tgatattaac
     1681 catattattg tggctgataa caaagttcca ggagatccaa tgtctgtgat ggctttgaca
     1741 attgctaaac ctgctgattg caaattagac attgttaaaa ttagtgatgt taaagggatt
     1801 atggaaacat ccccaaatga taattacatg attttaatta aaggacctaa ggaagctttt
     1861 gaattagttc aaaatttacc agaaattaaa gaaattaatt ttggcggtgt gtctaagaaa
     1921 agcgagtcca aacaatatgg aaaggcagtc tttttaactc ctgaagaatt agaagccaca
     1981 aaaaaaattc ttgatcaaga agtgaaaata tttgtgcaac aagtcccatc ttcaagtatt
     2041 gaaaatgtag attttagtaa gtaagggggg aaaataaatg ttattaaaag ccattttatt
     2101 aggtttagtt ggtgtggtag ctgttattga ctccaggtta attggtcgac aaaatattgg
     2161 tagaccacta attttgagta cacttgccgg acttgtgcta ggtgacttgc atacagggat
     2221 tgttcttggg gcatctttag aattaatttc tatgggattc gtctcaattg gggcggctgg
     2281 acctcctaat atgcaacttg gtagtattat tgccactgct tttgcaattt taactaatag
     2341 tagtacagaa acagccttaa caatagctat cccagtggct gtagctggtg aatttatgag
     2401 tattatcatg cgtatgataa ttgctcaatt tggacatgct gcagataaag ccattgaaaa
     2461 taataaattt agaaaagctc aaattattca tatttattgg tcatttattt ttaatgcttt
     2521 tgtttacttt attccaatct ttttaacagt atattttggt gctgacgttg tggcagattt
     2581 agtagataaa attccagaaa tgattacaga cgccttgact gtctctggaa atatgctttc
     2641 cgcattaggg tttgcaatgc tattaagcac aatgttgagt aaaaaattat atccttactt
     2701 tatttttgga ttctttattg ttgcctattc aggtctttca ctaattggcg tgacaatttt
     2761 tgcagctctg attgcctttg taatggatca agttaaatat ggaaaaagag aagaggcgca
     2821 tggttaatgg aaaatagtac agaaaaaaaa cgtttaatca caaaaaaaga tcttcataag
     2881 atgtgtcttc gttcatggac aatcatgtgt tcgtggaatt atgaaagaca aatgcatatg
     2941 ggatttatgt atgggatggc gccaatttta aataaagttt ataaaaatga tgaagagaaa
     3001 aaaagagaag cttatactcg tcatatggca ttttttaact gtacgcctca aatgactcca
     3061 tttattatgg gactttgtgc ttctatggaa gaacaaaatg ctcaagcaga agaaggaaac
     3121 tttaatactg aatctatctc catgattaaa accagtttaa tgggtccttt tgctggaatt
     3181 ggtgattcat ttttccaagg tactttaaga attattacat ttgggattgg attgtctttt
     3241 gctaagcaag gtagtatttt aggaccgatt ttagctgttg gcctttttgc tataccttct
     3301 attttagctg ttttttacgg cggtaaatta ggatatgagt ctggcaataa ataccttact
     3361 aaattgtatc aagaaggtct tatggatcga gtaatgcaat ttgcttctat cattggtttg
     3421 gctgtagtag gtggaatggt ggctagcatg gtgccaattg tcaccccttt aaaattcact
     3481 acagggggaa cagaacttgt tttacaagac ttacttgatt ccattattcc tggattatta
     3541 ccacttgtat gtactttggg aatatataaa ttgattaaga aaaatgttag tacaaatatg
     3601 ctcttattag gttgtatttt ggcagggttt gttttaagta taataggtat cttataatga
     3661 ttaaggctct aatttttgat atggacggag ttttaattga tagcgagatt atgtatcaaa
     3721 agtgggtatt taatttttta aaacaagaaa atttaagcta tcctttgtat tcttatcaaa
     3781 aaaagattgg aactacagca agtatttttg atgatataga aaagtacaat gagggatgta
     3841 acagtttaga attgaaggga aaatttacta aatattggaa atcgcaaaag attgattact
     3901 tggatatttt tcctaatata ctaattgaac agcttgaatg gttaaaaaag aaaaaaataa
     3961 aaatggcaat tgcttcttcc agtccaatgg atactataaa tgaagttgtt accacttgta
     4021 aaatagagtc atattttgat tgtttaatta gcggaagaga tttgccagaa agtaaaccta
     4081 atcctactat attccttaaa gcttcagaac agctcagaat tccagttgaa gaatgtctgg
     4141 tgatagaaga ttcttataat ggtataaaag ctggtaaaag agcaaatatg aaggttcttg
     4201 caattaaaga taaacgtttt tctcaagatg taagtctagc agatgatgtt atttacgata
     4261 ttaaatatct gcgacaagaa gtacaggtga aatttggaat ataaataagt tttttgatgt
     4321 atttagagaa tattagtaaa aataaaaatc atgcccaaat ctaatcaaaa ttgattgctt
     4381 tgggtatgat ttcttttaat atcaatctat tactttagga tctgaatcat ttttatgcac
     4441 cgtatctaaa gtgatatttt ctgtgacttc aataacataa ctaattctat cacaacgaac
     4501 tgtggattga gaataatgaa atggttgatt atttttatct ttgccaacct tttctaaaat
     4561 aaatagcgga tctcctacta tacattgcaa taagtcggaa agctcaggtg ttgcagataa
     4621 accgtttatt tccataatag agctcgtagc tttgacatca tagtcatctt ctagaagttt
     4681 atataatgaa gtatattggc tacctttttc aaataaagtt ggaaattttt cttctgatgc
     4741 gaaaaaatta tcaattccca tcggggcacc gtcttcccac attaaacgtt taatattgta
     4801 aactctatca gttacattga tttgtaattt attagctaaa aactcatcag cctttataat
     4861 tttagaatgt aaaacagtat atctaatttt atgtcctcgg gtagaaagag aatcgctaaa
     4921 tccatgaact ccagctaatt ttaaactttc ttttttttga gttacaaagg tccctttccc
     4981 ccttaatcgt tcaactgttc cactttctgt taaaagttta aaagcttttc gaatggtaat
     5041 cttacttact ccataaattt tacataattc ttcttctgta ggtaattgct ctcctggaag
     5101 atattttttt tcacgaatat cgttacgtat agaagcttct acttgtagat ataatggtct
     5161 attatcttga aaattcatag tacactccct aaaaagttat acttcattat atcacaattt
     5221 attaaaataa aagattaaga tataaatata aatgaactag tctttgacta accaatgccc
     5281 gcttttgaaa gaaaacaaat tcattctgtt ttaagtaaaa atccttatgt taccactcat
     5341 tctgaagggg atgagcctta tcgctacttg gttgtggaac caagcgataa gacaatttta
     5401 taaattcaat attaattaaa ataaaaacaa acaaagtaga ttattttact ttgtttgttt
     5461 ttttat
//
LOCUS       DACGLE010000117         5250 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0534
            SAMEA3304449-rid9560453.denovo.123, whole genome shotgun sequence.
ACCESSION   DACGLE010000117 DACGLE010000000
VERSION     DACGLE010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304449
            Sequence Read Archive: ERR985064
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5250)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5250)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702225.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702225.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 20:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,045
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 27 of 67
            Pseudo Genes (incomplete)         :: 25 of 67
            Pseudo Genes (internal stop)      :: 19 of 67
            Pseudo Genes (multiple problems)  :: 4 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5250
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304449-rid9560453.denovo.123"
                     /strain="VREN0534"
                     /db_xref="taxon:1352"
     gene            24..1316
                     /locus_tag="IXT20_002650"
                     /pseudo
     CDS             24..1316
                     /locus_tag="IXT20_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748779.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
     gene            1835..2569
                     /locus_tag="IXT20_002651"
     CDS             1835..2569
                     /locus_tag="IXT20_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ4861057.1"
                     /translation="MQNTFQSKSLYYQLVEILKQRIENSMVAHDKLPSERELTSFYGV
                     SRTTVRLALQELEKMGYIYRQHGKGTFVSELRTNATDLAGAYSFTEQMRAMGKHPETK
                     ILAFKKIEATKYLASHLNVQLGEPLFQLDRLRLADDLPMMIEKSYLPVKLFMSLSQEI
                     LFLKPLYNLFSENFNQIVRLADEEFYASIVQKEDSELLNIPEGSPVLHLVRSTYNLEN
                     EVIEFTLSVARADKFHYKIRHLRSTN"
     gene            2585..3754
                     /locus_tag="IXT20_002652"
     CDS             2585..3754
                     /locus_tag="IXT20_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAQ4861058.1"
                     /translation="MFMFSKEELRKLGAENTTREIRQQPELWHETFDRYQETVADLDR
                     LFTDAKQHAGERPIRVIFTGAGTSQYVGDTLIPFLTSQGNTNQFIFQSIGSTDIVATP
                     KEYLIPDETTILVSFARSGNSPESLAAVKMANQLVNKIYHIVITCAPDGLLATQMPTK
                     KENLVLLMPNKANDAGFAMTSSFSCMTLTALLIFDQASNQQKERYVHQLSRLGEEVIQ
                     RESEIQGYLASDFHRIIYLGSGSLGGLTREAQLKVLELTAGKIATVFDTSLGFRHGPK
                     SFVNEQTLVFLFVSNDSYTQKYDVDMLEEIKNDGIAQRVVGIGQKGSLFSGDSFELVA
                     DSLLPDGYLALADIMVAQTIALLCSVKVGNTPDTPSPSGTVNRVVKGVTIYPYEK"
     gene            3770..4765
                     /gene="lacD"
                     /locus_tag="IXT20_002653"
     CDS             3770..4765
                     /gene="lacD"
                     /locus_tag="IXT20_002653"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HAQ4861059.1"
                     /translation="MLEWSENKRQAMERLSTPEGIITALAIDQRGALKKMMSALGMEA
                     TKEKIEQFKTLISQELTPYASAILLDPEYGLPAAQKRAAASGLLLAYEKTGYDQTVPG
                     RLPDLLSDCSVLRLKELGADAVKFLLYYDYEEAEEINRQKQVFVERIGSECMAEGLPF
                     YLELLSYDAKNVDTKSLSYAKAKPRKVIEMMKEFSKTQYKVDVLKVEVPVNMSFVEGN
                     ATGEIAYTRQEAAAYFKEQSDATDLPYIFLSAGVSAALFMETLRFAKHAGARFNGVLC
                     GRATWKDAVAPFVEKGEAATLEWLTEQGTQNIRQLNEVIRETAIPWYEKVSESTC"
     gene            4844..>5250
                     /locus_tag="IXT20_002654"
     CDS             4844..>5250
                     /locus_tag="IXT20_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase-like hydrolase"
                     /protein_id="HAQ4861060.1"
                     /translation="MNKRLLDYSASDFLKAKPMELKQAILASEGRTIMAENVPSSCPY
                     MGKSVTNAELERAAGADLILFNALDLFNPQISGVPLTLKENPIIWIKKALGRAIGVNL
                     EPVDKEVLMSENRTEISSGRIVSPETLKKANELG"
BASE COUNT         1724 a          911 c         1135 g         1480 t
ORIGIN      
        1 taagatcaag aggtgattac acgatggcta gaaagaaaag aaatcctgat gccgaaaagt
       61 tagctgaatc cattctgaat gcctatcaac ctgaatctgt cgatgacatg caagatgctt
      121 tgaaagatgt gtttgggccc ctttttgaaa aaatgcttca aggagaattg aataatcatt
      181 taggttatga tgcccattct aaagagccta aggaacacga taaccgtcga aatggctatg
      241 gaactaaaac gcttaaaacc agttttggtg aagtagctat tgatgttcct agagaccggg
      301 aagcttcctt tgaaccagag ttaattccta agagaaaacg agatgtctcc gacatcgaag
      361 ggaaggttct ttccatgtat gcacgaggaa tgagtcaatg agatattgcc gcaaccgtcg
      421 aagctattta tggctttgat atttcccatg aaatgatttc agatatcact gacgctgtcc
      481 ttcctgaatt ggaagaatgg caagcccgcc ccctagcaaa gtgttatgcc tttctattcg
      541 ttgattgtat gtatgttact ttacgagaaa attatgaagc caaagaatat gctgtacaca
      601 ccattcttgg ctatgatctc aaaggaaata aggagatttt aggattatgg ttaaatcaaa
      661 cagaatctaa aaatcgctgg atgcaagtat ttgatgaact gaaagcacgt ggtgtcgaag
      721 atgtcttttt catttcgatg gacggtgttt ctggcctcga agaaggcgca aaagcgatct
      781 ttccatcggt aattgttcag cgctgtattg ttcatcttgt tcgaaatgca ttgcgctata
      841 ttccaagtaa ggactataag gaagtctgcc gagatatgaa aaagttctac ggtgcttctt
      901 ctctaaacgc tgcacatgct gcttttgaca gttttcaaaa tcggtggtct cattattctg
      961 gtgctgtaga tgtctggaaa cggaactttg cacatgttga acaactattt gattatggta
     1021 gtgccattcg aaaaattatg tacactacca atgcggttga aagtgtccac tccagcttca
     1081 gaaaagtcac aaaaaaagga gcgttctcta atgagaatgc actcctaaaa ctactttatt
     1141 tacgcacgaa agaattacac gcgaagtggt caggtggtcg aattcaaaac tgggctatgg
     1201 tgcttaatca gttaatgatc aacgagacct tctcctctag aatgaaaagt atgcgattta
     1261 ccttccgtaa caacggtagg tttattcaac tatgtgtccg atcaaaattt atctgatttc
     1321 aaaatagccg agagaattat ggtggttaaa aactcttaac tatttaactt ccacagttta
     1381 gttgacaaac ccacttaatt ctgataacga aaaacatatt cgaatgatta aacagaagta
     1441 aatacttatt taaataactt ttaattttca tgttcataaa tgatttgaaa ccaaagcaaa
     1501 tgcgttgcac ttttcctccg tcttccgaaa atcgttcaat tcactggtaa gtagtcattt
     1561 gatagtttga ggcaaattgt tgattgatga agggtaggag ctaaaaaaag ataccagttg
     1621 aagagagaaa aagaaaaaaa tagagggtgt tcttgaatga aaaaagttgg aaaattattt
     1681 ttcatttcct tttattccta ttaaaatcag tgatgtcaat catattaaca agactaaaaa
     1741 tgaaaataaa aaaaagattg ttcttagtat tgactcataa aaaataccac tactatactt
     1801 acattgtggt tatagccagt taggagagac atcgatgcaa aatacctttc aatcaaaatc
     1861 actttattat cagctggtag aaatattgaa acaacgaatc gaaaacagta tggtcgctca
     1921 tgacaagctg ccatcagaaa gagagttgac cagtttttat ggcgttagtc gcacgacggt
     1981 gcgactagca ttgcaagaac tggaaaaaat gggctacatt tatcgacagc atggaaaagg
     2041 aacctttgtt tcggaattaa ggacaaatgc gactgatcta gctggcgcat atagttttac
     2101 cgaacaaatg cgggcaatgg gcaaacatcc tgaaaccaag atattggcct tcaagaagat
     2161 tgaagcaact aaatatttag cgagtcatct gaatgttcaa ctaggtgagc ctttatttca
     2221 attagaccga ttgcgcttag ctgatgattt gccaatgatg atcgagaaat cctatttgcc
     2281 agtgaagcta tttatgtccc tttctcaaga aattctattt ctgaaacctt tatataattt
     2341 gttttcagag aatttcaatc aaatcgtacg gttggctgac gaggaatttt atgccagcat
     2401 tgtccagaaa gaagatagtg agttgctaaa tattccagaa ggatcaccag tgctccattt
     2461 agttcgctca acctataatc tcgagaacga agtgattgaa tttacattaa gtgtagcgcg
     2521 ggctgataaa tttcattata agatcaggca tctgcgttcc actaactgaa ttggaggaaa
     2581 gaaaatgttt atgttctcaa aagaagaact acgaaagcta ggagcagaaa atactacccg
     2641 agaaattcga caacaaccgg aactgtggca tgagacgttt gaccgttatc aagaaacagt
     2701 tgcagattta gatcgtttgt ttacggatgc gaaacaacat gctggagagc gaccaatccg
     2761 agtcattttt acaggggccg gaacctcgca atatgtggga gatacactaa ttccgtttct
     2821 tactagtcaa ggaaatacga accagtttat ttttcaaagt attggttcga ccgatatcgt
     2881 tgctacgcca aaagaatacc tgattcctga tgagacaacg atcttggtct catttgcccg
     2941 tagtggaaat agtccagaaa gtctagccgc agttaagatg gccaatcagc tggtcaacaa
     3001 gatctatcat atcgtgatca cctgtgcacc agacggatta ttggctacac aaatgcctac
     3061 aaaaaaagaa aatctagttt tactaatgcc aaacaaagcc aatgacgctg gttttgctat
     3121 gacaagcagt ttcagctgta tgacgctgac tgcgctactt atttttgatc aagcaagcaa
     3181 tcagcaaaaa gaaagatatg ttcatcaatt aagccgacta ggagaagaag tcatccaacg
     3241 agaatcagag atccaagggt atctggcatc agactttcat cgcatcattt acttaggttc
     3301 gggaagttta ggcggattga ctagagaagc gcaattaaaa gttctcgagt taactgctgg
     3361 aaaaatcgca accgtatttg atacatcttt aggattcaga catggtccca aatcttttgt
     3421 caatgagcaa acattggttt tcctgtttgt cagcaatgac tcatatacgc agaaatacga
     3481 tgtagacatg ctggaagaaa tcaaaaatga cggtattgcc caacgcgttg ttggaattgg
     3541 tcaaaagggt tcattgttta gtggtgattc ctttgaatta gttgcagatt cgttactgcc
     3601 ggatggctat cttgctttag cagatatcat ggtagcccaa acgatcgcat tactttgttc
     3661 ggtgaaggtt ggtaatacac cagatacacc atcgccaagt ggaacagtca atcgtgtggt
     3721 caaaggcgta acgatttatc catatgaaaa ataggaaaga gtgtgaagca tgttagaatg
     3781 gtcggaaaat aaaagacaag caatggaacg tctatcgacg ccagaaggca ttattaccgc
     3841 tttggcgatc gatcagcgag gcgcgctgaa gaaaatgatg tcggctttag ggatggaagc
     3901 gacaaaggaa aaaattgagc agtttaaaac actaatttcc caagaattga ctccttatgc
     3961 ctcagcaatt ttattggatc ctgaatacgg actgcctgct gcacagaagc gtgcagcagc
     4021 gagcggtctg ctgttggcat atgaaaaaac tggttatgat caaaccgtac ccggccgctt
     4081 gccggatctg ctgtcggact gttcagttct gcgattgaaa gagttaggtg cggatgcggt
     4141 gaaattttta ctgtattacg attatgaaga agcagaggaa atcaatcggc aaaagcaagt
     4201 ttttgttgaa cgaattggaa gcgaatgcat ggctgaagga ctgccatttt atttagagtt
     4261 attgtcatat gatgctaaaa atgtagatac gaaatcgctt tcctatgcca aagcaaaacc
     4321 gcgcaaagtg atcgaaatga tgaaagaatt ctcgaaaacc cagtataaag tagatgtctt
     4381 aaaggttgaa gtaccagtaa acatgtcatt tgttgaagga aatgcaacag gggagatcgc
     4441 ctatacacgt caagaagctg ccgcttattt taaagaacaa agcgatgcaa cagatttgcc
     4501 gtatatcttc ttaagtgctg gggtatcagc ggcattattc atggagacat tgcgttttgc
     4561 caaacatgct ggggcacgat tcaacggtgt actctgtggc agagcaactt ggaaagatgc
     4621 agtcgctcct tttgttgaaa aaggagaagc ggcaacttta gaatggctga cagaacaagg
     4681 gacacaaaac attcgtcaat tgaatgaagt gataagagaa actgccatcc catggtatga
     4741 gaaagtaagt gaaagcactt gttaatgttt ctaatatatt agtagtgata agagatatgt
     4801 gtgattataa tttatcatta ttgaaactgg gaggatattt tatatgaata aaagattgtt
     4861 agattattca gcttcagatt ttttgaaagc taagccgatg gaattaaagc aggcaatttt
     4921 ggcttctgaa ggtagaacaa ttatggcaga aaatgtgcca agtagctgtc cttacatggg
     4981 aaaatcagtg acaaatgcag aattggagag agctgctgga gctgatttaa ttttgtttaa
     5041 tgcactagat ctttttaatc ctcaaatttc aggtgtacct ttaactctaa aagagaatcc
     5101 aataatctgg attaaaaaag ctttaggaag agctattggt gtaaatttag aacctgtaga
     5161 caaagaagtt ttgatgagtg aaaatcgaac tgaaatttca agtggtagaa tcgtttctcc
     5221 tgagacttta aaaaaggcaa atgaattagg
//
LOCUS       DACGLE010000118         5157 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0534
            SAMEA3304449-rid9560453.denovo.124, whole genome shotgun sequence.
ACCESSION   DACGLE010000118 DACGLE010000000
VERSION     DACGLE010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304449
            Sequence Read Archive: ERR985064
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5157)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5157)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702225.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702225.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 20:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,045
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 27 of 67
            Pseudo Genes (incomplete)         :: 25 of 67
            Pseudo Genes (internal stop)      :: 19 of 67
            Pseudo Genes (multiple problems)  :: 4 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5157
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304449-rid9560453.denovo.124"
                     /strain="VREN0534"
                     /db_xref="taxon:1352"
     gene            complement(<1..580)
                     /locus_tag="IXT20_002655"
     rRNA            complement(<1..580)
                     /locus_tag="IXT20_002655"
                     /product="16S ribosomal RNA"
     gene            500..643
                     /locus_tag="IXT20_002656"
     CDS             500..643
                     /locus_tag="IXT20_002656"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4861061.1"
                     /translation="MEKEAFDLHVLGTPPAFVLSQDQTLIKSSNNLAIVKLNLFASIL
                     LAC"
     gene            complement(1266..2771)
                     /locus_tag="IXT20_002657"
     CDS             complement(1266..2771)
                     /locus_tag="IXT20_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAQ4861062.1"
                     /translation="MIKPKKSEKRELKGPSLTIKWAFASSFFIFVVFTIFAVITYKSS
                     INLIVAKERNNVERTISEVASRLANSDEELTLVNTYRNLTKATNDGDEVVDHTSLVEG
                     NLMKIDSFIAELGQPSMDLYVYDLDENLIFKTHENSRNLIQTTRKVPTIVTLEGKTGF
                     LSIQPIYSKGTREKIGYAQSFYELSSFYDIRNKLLLTLIVLEVVSLILSSILGFFLSS
                     YFLKPLKVLRDTMDTIRKDPQSDIHMPEIDTNDELADLAEIFNEMLDRMRLYIEQQEQ
                     FVEDVSHELRTPVAIIEGHLSLLNRWGKDDPEILEESLSASMQEISRMKSLVQEMLDL
                     SRAEQVDVHYANETTKGKEVTYQVYNNFKILYPDYVITLDDDLTKEVTLQIYRNHFEQ
                     LIIIILDNAVKYSTTRKEVHISISSTLNEFEIAIQDFGEGIPKEDLNKIFNRFYRVDK
                     ARARTKGGNGLGLSIAKQLVENYKGRILAESVVGQGTIFRIFIPIAKAEDK"
     gene            complement(2768..3454)
                     /locus_tag="IXT20_002658"
     CDS             complement(2768..3454)
                     /locus_tag="IXT20_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010763438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ4861063.1"
                     /translation="MSNILIIEDEKNLARFVELELKHEGYNAEVHYNGRTGLDAALNN
                     EWDAILLDLMLPELNGLEVCRRVRQVKNTPIIMMTARDSVIDRVSGLDHGADDYIVKP
                     FAIEELLARLRALLRRIDIEGDKNVAKQTTITYRDLTIEKENRVVRRGNEVIELTKRE
                     YELLLTLMENVNVVLARDVLLNKVWGYETEVETNVVDVYIRYLRNKIDVPGEESYIQT
                     VRGTGYVMRS"
     gene            complement(3647..5068)
                     /gene="gndA"
                     /locus_tag="IXT20_002659"
     CDS             complement(3647..5068)
                     /gene="gndA"
                     /locus_tag="IXT20_002659"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HAQ4861064.1"
                     /translation="MSKQQIGVVGMAVMGKNLALNIESRGYSVALFNRTGAKTTAVVE
                     EHPDKNFKATYTIEEFVESIEKPRRILLMVKAGPATDATIQELLPHLDKGDILIDGGN
                     TFFKDTMRRNEELANSGINFIGTGVSGGEEGALKGPSIMPGGQKEAYALVAPILEQIS
                     AKAEDGAPCVTYIGPNGAGHYVKMVHNGIEYGDMQLIAESYDLMKNILNLSVEEMADI
                     FKEWNQGELDSYLIEITADILTRKDDEGTGKPVVDVILDAAGNKGTGKWTSQSALDLG
                     VPLPLITESVFARYISAYKEERVQASKILSRTNDFEFTGDKKELVEKIREALYFSKIM
                     SYAQGFAQLRVASKEFDWDLPFGEIAKIWRAGCIIRARFLQKITDAYDKNPEIENLLL
                     DDYFVEITKKYQQSVRDVVALAVQAGVPVPTFSSAIAYFDSYRAERLPANIIQAQRDY
                     FGAHTYERVDKEGIFHYSWYHEE"
BASE COUNT         1397 a         1114 c          886 g         1760 t
ORIGIN      
        1 aaatccggac aacgcttgcc acctacgtat taccgcggct gctggcacgt agttagccgt
       61 ggctttctgg ttagataccg tcaagggatg aacagttact ctcatccttg ttcttctcta
      121 acaacagagt tttacgatcc gaaaaccttc ttcactcacg cggcgttgct cggtcagact
      181 ttcgtccatt gccgaagatt ccctactgct gcctcccgta ggagtttggg ccgtgtctca
      241 gtcccaatgt ggccgatcac cctctcaggt cggctatgca tcgtggcctt ggtgagccgt
      301 tacctcacca actagctaat gcaccgcggg tccatccatc agcgacaccc gaaagcgcct
      361 ttcaaatcaa aaccatgcgg ttttgattgt tatacggtat tagcacctgt ttccaagtgt
      421 tatccccttc tgatgggcag gttacccacg tgttactcac ccgttcgcca ctcctctttt
      481 tccggtggag caagctccgg tggaaaaaga agcgttcgac ttgcatgtat taggcacgcc
      541 gccagcgttc gtcctgagcc aggatcaaac tctcataaaa agttcgaaca atcttgcgat
      601 tgttaagctc aatttgtttg ctagcatatt gcttgcttgt taaaaatgtt tgttgtcttg
      661 aattgaatca agacgcccta cacatttggt ttgtcttact ttgttcagtt ttcaaaggtc
      721 taatcttttt gttttaacaa ctctttaatc ttatcagatt cagaagagaa tgtcaacaac
      781 ttctttgttt attttttcta aacgtctttg ccactcatta gcagcgactt gattatcata
      841 ccatctttat tgttatttgt caataacttt ttcgaagttt ttttcgataa aatcaagctt
      901 tattagctat ctcatccaaa cgacttcgtt attataacat gacatcaaaa gtgcgtcaac
      961 actatttttt ataattttta gtgacacctt aaaggaatca tttagaacaa ataataatat
     1021 agcaatctct ttataaaatt gcaatagaaa attccctatt tttatttatt atttttaaac
     1081 acgaatatta cagcatttta ttgttttttc gttcatctaa aagatactta catttaatta
     1141 acggatgtta ttctatttaa ttgttcgcta gtttttttat aatgacatcc ctgaatttct
     1201 cacttgcttg tattttgtac cttattcttt gttggatata taaaaaaaca agtaaagcac
     1261 atgctttact tgtcttctgc ctttgcgata ggaataaaga ttctaaaaat tgtcccttgc
     1321 cctactacac tttctgctaa gatacgtcct ttatagtttt caaccagttg tttagcgata
     1381 gacaacccca gaccatttcc acccttcgtt ctagctcttg ctttatccac tcgataaaaa
     1441 cgattgaaga tcttattaag gtcttctttg ggtattcctt cgccaaagtc ctgaatcgcg
     1501 atctcaaatt cattcagagt ggaagaaata gagatatgga cttcttttcg tgtagttgaa
     1561 tactttactg cgttatccaa tatgataatg attaattgtt caaagtgatt acgataaatt
     1621 tgcagggtca cttcttttgt caaatcatca tctaaagtga tcacatagtc tggatataat
     1681 atcttgaaat tattataaac ttgataggtg acttcttttc ctttagttgt ttcattcgca
     1741 taatgaacat ctacttgttc tgctcttgat aagtctagca tctcttgaac taagcttttc
     1801 atacggctga tttcttgcat gctggcgctt aatgattctt ctaatatctc aggatcatct
     1861 ttcccccaac gatttagcag actaagatgc ccttcaataa ttgcaacagg tgtccttaac
     1921 tcatgagata cgtcttccac gaattgctct tgttgttcaa tatacaaacg catacggtca
     1981 agcatctcat tgaatatttc agctaaatct gccaattcat catttgtgtc gatttcaggc
     2041 atgtggatat ctgattgcgg atctttacgt atagtatcca ttgtatctct gagtaccttg
     2101 agtggtttta agaaataaga agataaaaag aaacctaaaa tactacttaa gatcaaagaa
     2161 acaacttcta aaacaataag agtcaataat agcttatttc gaatatcata gaaagaagat
     2221 aattcataga agctttgcgc atagccgatc ttttctcttg tacctttaga atatataggc
     2281 tggatagata aaaagccagt ttttccttct agtgtaacga tcgttggaac tttacgtgta
     2341 gtctgaatca ggtttcgact gttttcatgg gttttaaaaa tcaagttctc gtccaaatca
     2401 taaacataaa gatccattga tggctgccct agctcagcta taaaggaatc gatcttcatt
     2461 aagtttcctt cgaccagtga agtatgatct acgacctcat ctccgtcatt tgtcgctttc
     2521 gtcagattac gatacgtatt tactaatgtc agttcttcat ccgagttagc caaacgagaa
     2581 gctacttctg aaatcgtacg ctctacattg tttctttctt ttgccacaat cagattgata
     2641 gatgatttat aggtaatgac agcgaaaatt gtaaacacaa cgaatatgaa gaaagaactc
     2701 gcaaaggccc acttgattgt caaggagggc cctttcagtt ctcttttttc cgattttttc
     2761 ggctttatca cgagcgcatc acataacctg ttccgcgtac agtttggata tagctttcct
     2821 ccccgggaac gtcaattttg ttgcgaagat aacgaatata tacgtccact acgtttgttt
     2881 caacttctgt ttcgtatccc cacactttat ttagtaaaac gtcacgtgct aataccacgt
     2941 tcacgttttc cattaaagta agcagcaact cgtattctct ttttgttagt tcaattactt
     3001 catttccgcg acgtacgaca cggttttctt tttcgatagt caaatcgcga taagtaatag
     3061 tagtttgttt agccacattt ttatcgcctt caatatcgat tcgacgcaac aatgcacgaa
     3121 gacgtgctaa taattcttcg atagcaaatg gtttgacgat gtagtcatct gctccgtggt
     3181 caagtccaga tacacgatcg atcacagaat ctcttgcagt catcataatg attggtgtat
     3241 ttttgacttg gcgtacacgt ctgcagactt ctaaaccgtt caattctggg agcatcagat
     3301 ccaacaatat ggcatcccac tcattattta atgccgcatc taaaccagtt cggccgttgt
     3361 agtggacttc tgcgttatac ccttcatgct tcaactctag ctcgacaaat cgtgccaagt
     3421 tcttttcatc ttcaatgatt agaatattgc tcatttatta ttttatcctt tctattctac
     3481 tggcttttta aaaactctat cattataccg ataatatgct taaaaatcta tagtcatttt
     3541 atcatggata gcaattttaa aaaagagctg aagtctactc ataacaatcg acttcagctc
     3601 tttcgtcctc tttttatttt ctcatttcct aacagccgaa agactattat tcttcatggt
     3661 accatgagta atggaagatt ccttctttgt cgacacgctc ataggtatgt gcgccaaagt
     3721 aatcgcgttg ggcttggatt atatttgcag gcagacgttc tgctctgtat gaatcgaagt
     3781 aagcgatggc tgatgagaaa gtcggtacag gtacgccagc ttggacagcc aaggcaacga
     3841 cgtctctgac tgattgctga tattttttcg tgatttcaac gaagtaatca tctagcaata
     3901 aattttcgat ctcaggattt ttgtcatagg catccgtaat tttttgcaag aatcgagctc
     3961 ggatgataca tccagcgcgc cagatttttg cgatttcacc aaatggcaag tcccaatcaa
     4021 attcttttga agcgacacgt aattgagcaa atccttgggc ataactcatg attttactga
     4081 aatacaacgc ttctcggatt ttttcgacca attctttttt gtctcccgtg aactcaaaat
     4141 cgttcgtacg agacaaaatc ttgcttgctt gtacgcgttc ttctttataa gcagaaatat
     4201 agcgggcaaa tacagattca gtgatcaatg gaagtggcac acctaagtca agcgcgcttt
     4261 ggcttgtcca tttacctgtt cctttattcc cagctgcatc aaggatcaca tctactactg
     4321 gtttacctgt tccttcatcg tctttacgtg tcagaatgtc agcagtgatt tctatcaaat
     4381 agctgtctaa ttcgccttga ttccattctt taaagatatc agccatttct tctactgaaa
     4441 ggtttaaaat attcttcatt aagtcataag attcagcaat cagttgcata tctccatact
     4501 caataccgtt gtgtaccatt ttcacataat gtccggctcc atttgggcca atatatgtga
     4561 cacatggtgc tccgtcttct gcttttgcag aaatttgctc taagattggt gcaactagcg
     4621 cataggcttc tttttgtcca ccaggcatga ttgaaggtcc ttttaaagct ccttcttcac
     4681 caccggaaac gcctgtaccg ataaagttga tgccagagtt ggctaactct tcattacgac
     4741 gcatcgtatc tttaaagaat gtatttcctc cgtcaatcaa aatatcgcct ttatcaaggt
     4801 gaggaagtaa ctcttggatt gttgcatctg ttgccggtcc tgctttgacc attagcaaga
     4861 tccgacgtgg tttctcgata gattctacaa attcttcgat tgtatatgtt gctttgaaat
     4921 ttttatctgg gtgttcttct actacagcgg ttgttttagc ccctgtacga ttgaataaag
     4981 ccactgaata gccgcggctt tctatgttca aggctagatt tttacccata acggccatac
     5041 cgacaacgcc gatttgttgt tttgacatat gaactcctcc tacatacggg atttctccct
     5101 aatctcttac attatagctt aacacggcta cattgcaaac taaagaacaa catattt
//
LOCUS       DACGLE010000119         5025 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0534
            SAMEA3304449-rid9560453.denovo.126, whole genome shotgun sequence.
ACCESSION   DACGLE010000119 DACGLE010000000
VERSION     DACGLE010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304449
            Sequence Read Archive: ERR985064
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5025)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5025)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702225.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702225.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 20:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,045
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 27 of 67
            Pseudo Genes (incomplete)         :: 25 of 67
            Pseudo Genes (internal stop)      :: 19 of 67
            Pseudo Genes (multiple problems)  :: 4 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5025
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304449-rid9560453.denovo.126"
                     /strain="VREN0534"
                     /db_xref="taxon:1352"
     gene            complement(<1..2287)
                     /locus_tag="IXT20_002660"
     CDS             complement(<1..2287)
                     /locus_tag="IXT20_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000684984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
                     /protein_id="HAQ4861065.1"
                     /translation="MKIARGRELLTPEQRQAFMQIPEDEWILGTYFTFSKRDLEIVNK
                     RRREENRLGFAVQLAVLRYPGWPYTHIKSIPDSVIQYISKQIGVSPSSLDHYPQRENT
                     LWDHLKEIRSEYDFVTFTLSEYRMTFKYLHQLALENGDAIHLLHECIDFLRKNKIILP
                     AITTLERMVWEARAMAEKKLFNTVSKSLTNEQKEKLEGIITSQHPSESNKTILGWLKE
                     PPGHPSPETFLKIIERLEYIRGMDLETVQISHLHRNRLLQLSRLGSRYEPYAFRDFQE
                     NKRYSILTIYLLQLTQELTDKAFEIHDRQILSLLSKGRKAQEEIQKQNGKKLNEKVIH
                     FTNIGQALIKAREEKLDVFKVLESVIEWNTFVSSVEEAQELARPADYDYLDLLQKRFY
                     SLRKYTPTLLRVLEFHSTKANEPLLQAVEIIRGMNESGKRKVPDDSPVDFISKRWKRH
                     LYEDDGTTINRHYYEMAVLTELREHVRAGDVSIVGSRQYRDFEEYLFSEDTWNQSKGN
                     TRLSVSLSFEDYITERTSSFNERLKWLAANSNKLDGVSLEKGKLSLARLEKDVPEEAK
                     KFSASLYQMLPRIKLTDLLMDVAHITGFHEQFTHASNNRKPDKEETIIIMAALLGMGM
                     NIGLSKMAEATPGLTYKQLANVSQWRMYEDAMNKAQAILVNFHHKLQLPFYWGDGTTS
                     SSDGMRMQLGVSSLHADANPHYGTGKGATIYRFTSDQFSSYYTKIIHTNSRDAIHVLD
                     GLLHHETDLNIEEHYTDTAGYT"
     gene            2433..3008
                     /locus_tag="IXT20_002661"
     CDS             2433..3008
                     /locus_tag="IXT20_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016204422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAQ4861066.1"
                     /translation="MRKIGYIRVSSTNQNPSRQFQQLNEIGMDIIYEEKVSGATKDRE
                     QLQKVLDDLQEDDIIYVTDLTRITRSTQDLFELIDNIRDKKASLKSLKDTWLDLSEDN
                     PYSQFLITVMAGVNQLERDLIRMRQREGIELAKKEGKFKGRLKKYHKNHAGMNYAVKL
                     YKEGNMTVNQICEITNVSRASLYRKLSEVNN"
     gene            3222..3917
                     /gene="vanR"
                     /locus_tag="IXT20_002662"
     CDS             3222..3917
                     /gene="vanR"
                     /locus_tag="IXT20_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance DNA-binding
                     response regulator VanR"
                     /protein_id="HAQ4861067.1"
                     /translation="MSDKILIVDDEHEIADLVELYLKNENYTVFKYYTAKEALECIDK
                     SEIDLAILDIMLPGTSGLTICQKIRDKHTYPIIMLTGKDTEVDKITGLTIGADDYITK
                     PFRPLELIARVKAQLRRYKKFSGVKEQNENVIVHSGLVINVNTHECYLNEKQLSLTPT
                     EFSILRILCENKGNVVSSELLFHEIWGDEYFSKSNNTITVHIRHLREKMNDTIDNPKY
                     IKTVWGVGYKIEK"
     gene            3895..>5025
                     /locus_tag="IXT20_002663"
     CDS             3895..>5025
                     /locus_tag="IXT20_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAQ4861068.1"
                     /translation="MVIKLKNKKNDYSKLERKLYMYIVAIVVVAIVFVLYIRSMIRGK
                     LGDWILSILENKYDLNHLDAMKLYQYSIRNNIDIFIYVAIVISILILCRVMLSKFAKY
                     FDEINTGIDVLIQNEDKQIELSAEMDVMEQKLNTLKRTLEKREQDAKLAEQRKNDVVM
                     YLAHDIKTPLTSIIGYLSLLDEAPDMPVDQKAKYVHITLDKAYRLEQLIDEFFEITRY
                     NLQTITLTKTHIDLYYMLVQMTDEFYPQLSAHGKQAVIHAPEDLTVSGDPDKLARVFN
                     NILKNAAAYSEDNSIIDITAGLSGDVVSIEFKNTGSIPKDKLAAIFEKFYRLDNARSS
                     DTGGAGLGLAIAKEIIVQHGGQIYAESNDNYTTFRVELPGFCC"
BASE COUNT         1571 a          948 c          973 g         1533 t
ORIGIN      
        1 cagtgtaacc ggcagtgtct gtataatgtt cctctatgtt tagatccgtc tcatgatgta
       61 acaaaccatc caaaacatga atcgcatctc ttgaattagt atgaataatc tttgtgtagt
      121 aagaagagaa ttgatcactt gtaaatcggt agatggtggc tccttttcca gttccataat
      181 gtggatttgc atctgcatgt agtgatgaaa cacctagctg cattctcata ccatctgacg
      241 aagatgttgt accgtcgccc caatagaaag gcaattgtaa tttatgatga aagtttacta
      301 atatggcttg ggctttattc atggcatctt catacatgcg ccattgagat acattggcta
      361 gttgcttata tgtaagtccg ggtgtggctt cggccatctt gctcaagcca atattcattc
      421 ccattcctaa aagggcagcc atgataatga ttgtttcttc cttatctggt tttcgattat
      481 tggaagcatg agtgaattgc tcatgaaatc ctgttatatg ggccacatcc atgagtaaat
      541 cagttaattt tattcttggt agcatctgat aaaggcttgc actaaatttt tttgcttctt
      601 ctggaacatc tttttctaag cgtgcaagtg atagctttcc tttttcaaga gaaaccccat
      661 ctaacttatt ggaattggca gctaaccact ttaacctttc attaaagctg ctggttctct
      721 ccgttatata atcttcgaat gataaactaa ctgataatct cgtattcccc ttcgattgat
      781 tccatgtatc ttccgaaaac aaatattcct caaaatccct atattgtctg ctgccaacaa
      841 tggaaacatc tcctgcccga acatgctccc gaagttctgt taaaacagcc atttcatagt
      901 aatgacgatt aattgttgta ccatcatcct cgtataaatg tcttttccat cgttttgaaa
      961 taaaatccac aggtgagtca tcaggcactt ttcgctttcc agattcgttc attcctcgga
     1021 taatctcaac agcttgtaaa agtggctcat ttgcctttgt agaatgaaat tccaatactc
     1081 ttaatagcgt tggcgtatat tttcttagtg aataaaaccg tttttgcagt aagtctaaat
     1141 aatcatagtc ggcaggacgt gcaagttcct gagcctcttc tactgaagag acaaaggtat
     1201 tccattcaat aaccgattct aaaaccttaa aaacgtctaa tttttcctct cttgctttaa
     1261 ttaatgcttg tccgatgttc gtaaagtgta taactttctc atttagcttt ttaccgtttt
     1321 gtttctggat ttcctcttga gccttacgac cttttgataa caaactaagt atttgcctat
     1381 catgaatttc aaacgcttta tccgttagct cctgagtaag ttgtaataaa tagatggtta
     1441 atatcgaata acgtttattt tcttgaaagt cacggaatgc atacggctcg tatcttgagc
     1501 ctaagcgaga cagctgcaac aggcggttac ggtgcaaatg actaatttgc actgtttcta
     1561 aatccattcc tcgtatgtat tcgagtcgtt ctattatttt tagaaaagtt tcgggtgaag
     1621 gatgacccgg tggctctttt aaccaaccca atatcgtttt attggattcg gatggatgct
     1681 gcgaggtaat aatcccttca agcttttctt tttgctcatt tgttagagat ttactaaccg
     1741 tattaaatag cttcttttca gccattgccc ttgcttccca caccattctt tcaagtgtag
     1801 tgatagcagg cagtataatt ttgttttttc ttagaaaatc tatgcattca tgcagtagat
     1861 gaatggcatc accattttcc aaagctaatt gatgaaggta cttaaatgtc attcgatatt
     1921 cactcagggt aaaagttaca aagtcgtatt cacttcgaat ttctttcaaa tgatcccaaa
     1981 gtgtattttc cctttgagga taatgatcaa gcgaggatgg actaacacca atctgtttcg
     2041 atatatattg tatgaccgaa tctgggatgc ttttgatatg agtgtatggc caaccgggat
     2101 accgaagaac agctaattga acagcaaatc ctaaacggtt ttcttccctc cttcgcttat
     2161 taactatttc taaatcccgt ttggaaaaag tgaagtaggt ccccagtatc cattcatctt
     2221 cagggatttg cataaaagcc tgtctctgtt ccggtgtaag caattctcta cctctcgcaa
     2281 ttttcattca gtatcattcc atttctgtat tttcaattta ttagttcaat tatatatcaa
     2341 tagagtgtac tctattgata caaatgtagt agactgataa aatcatagtt aagagcgtct
     2401 cataagactt gtctcaaaaa tgaggtgata ttttgcggaa aatcggttat attcgtgtca
     2461 gttcgactaa ccagaatcct tcaagacaat ttcagcagtt gaacgagatc ggaatggata
     2521 ttatatatga agagaaagtt tcaggagcaa caaaggatcg cgagcaactt caaaaagtgt
     2581 tagacgattt acaggaagat gacatcattt atgttacaga cttaactcga atcactcgta
     2641 gtacacaaga tctatttgaa ttaatcgata acatacgaga taaaaaggca agtttaaaat
     2701 cactaaaaga tacatggctt gatttatcag aagataatcc atacagccaa ttcttaatta
     2761 ctgtaatggc tggtgttaac caattagagc gagatcttat tcggatgaga caacgtgaag
     2821 ggattgaatt ggctaagaaa gaaggaaagt ttaaaggtcg attaaagaag tatcataaaa
     2881 atcacgcagg aatgaattat gcggtaaagc tatataaaga aggaaatatg actgtaaatc
     2941 aaatttgtga aattactaat gtatctaggg cttcattata caggaaatta tcagaagtga
     3001 ataattagcc attctgtatt ccgctaatgg gcaatatttt taaagaagaa aaggaaacta
     3061 taaaatatta acagcctcct agcgatgccg aaaagccctt tgataaaaaa agaatcatca
     3121 tcttaagaaa ttcttagtca tttattatgt aaatgcttat aaattcggcc ctataatctg
     3181 ataaattatt aagggcaaac ttatgtgaaa gggtgataac tatgagcgat aaaatactta
     3241 ttgtggatga tgaacatgaa attgccgatt tggttgaatt atacttaaaa aacgagaatt
     3301 atacggtttt caaatactat accgccaaag aagcattgga atgtatagac aagtctgaga
     3361 ttgaccttgc catattggac atcatgcttc ccggcacaag cggccttact atctgtcaaa
     3421 aaataaggga caagcacacc tatccgatta tcatgctgac cgggaaagat acagaggtag
     3481 ataaaattac agggttaaca atcggcgcgg atgattatat aacgaagccc tttcgcccac
     3541 tggagttaat tgctcgggta aaggcccagt tgcgccgata caaaaaattc agtggagtaa
     3601 aggagcagaa cgaaaatgtt atcgtccact ccggccttgt cattaatgtt aacacccatg
     3661 agtgttatct gaacgagaag cagttatccc ttactcccac cgagttttca atactgcgaa
     3721 tcctctgtga aaacaagggg aatgtggtta gctccgagct gctatttcat gagatatggg
     3781 gcgacgaata tttcagcaag agcaacaaca ccatcaccgt gcatatccgg catttgcgcg
     3841 aaaaaatgaa cgacaccatt gataatccga aatatataaa aacggtatgg ggggttggtt
     3901 ataaaattga aaaataaaaa aaacgactat tccaaactag aacgaaaact ttacatgtat
     3961 atcgttgcaa ttgttgtggt agcaattgta ttcgtgttgt atattcgttc aatgatccga
     4021 gggaaacttg gggattggat cttaagtatt ttggaaaaca aatatgactt aaatcacctg
     4081 gacgcgatga aattatatca atattccata cggaacaata tagatatctt tatttatgtg
     4141 gcgattgtca ttagtattct tattctatgt cgcgtcatgc tttcaaaatt cgcaaaatac
     4201 tttgacgaga taaataccgg cattgatgta cttattcaga acgaagataa acaaattgag
     4261 ctttctgcgg aaatggatgt tatggaacaa aagctcaaca cattaaaacg gactctggaa
     4321 aagcgagagc aggatgcaaa gctggccgaa caaagaaaaa atgacgttgt tatgtacttg
     4381 gcgcacgata ttaaaacgcc ccttacatcc attatcggtt atttgagcct gcttgacgag
     4441 gctccagaca tgccggtaga tcaaaaggca aagtatgtgc atatcacgtt ggacaaagcg
     4501 tatcgactcg aacagctaat cgacgagttt tttgagatta cacggtataa cctacaaacg
     4561 ataacgctaa caaaaacgca catagaccta tactatatgc tggtgcagat gaccgatgaa
     4621 ttttatcctc agctttccgc acatggaaaa caggcggtta ttcacgcccc cgaggatctg
     4681 accgtgtccg gcgaccctga taaactcgcg agagtcttta acaacatttt gaaaaacgcc
     4741 gctgcataca gtgaggataa cagcatcatt gacattaccg cgggcctctc cggggatgtg
     4801 gtgtcaatcg aattcaagaa cactggaagc atcccaaaag ataagctagc tgccatattt
     4861 gaaaagttct ataggctgga caatgctcgt tcttccgata cgggtggcgc gggacttgga
     4921 ttggcgattg caaaagaaat tattgttcag catggagggc agatttacgc ggaaagcaat
     4981 gataactata cgacgtttag ggtagagctt ccagggttct gttgc
//
LOCUS       DACGLE010000120         4861 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0534
            SAMEA3304449-rid9560453.denovo.127, whole genome shotgun sequence.
ACCESSION   DACGLE010000120 DACGLE010000000
VERSION     DACGLE010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304449
            Sequence Read Archive: ERR985064
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4861)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4861)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702225.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702225.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 20:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,045
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 27 of 67
            Pseudo Genes (incomplete)         :: 25 of 67
            Pseudo Genes (internal stop)      :: 19 of 67
            Pseudo Genes (multiple problems)  :: 4 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4861
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304449-rid9560453.denovo.127"
                     /strain="VREN0534"
                     /db_xref="taxon:1352"
     gene            complement(220..972)
                     /gene="xth"
                     /locus_tag="IXT20_002664"
     CDS             complement(220..972)
                     /gene="xth"
                     /locus_tag="IXT20_002664"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HAQ4861069.1"
                     /translation="MKFISWNVNGLRAIVNKNFLEVFHELDADFFCLQETKLQAGQID
                     LDLPGYYQYWNYAERKGYSGTAIFAKKPALNATYGMGIDIHDTEGRLITLEYSDFFLV
                     TCYTPNSQSELKRLDYRLEWEEAFYNYLENLKKQKPVIVCGDLNVAHQKIDLKNWKTN
                     QKNAGFTPEERAALSRLLDNGFIDTFRYFYPTQEGVYSWWNYRFNSRKNNAGWRIDYF
                     LTSKDLEPGLADAKIHTDIMGSDHCPVELDLK"
     gene            1085..2005
                     /locus_tag="IXT20_002665"
     CDS             1085..2005
                     /locus_tag="IXT20_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAQ4861070.1"
                     /translation="MKIGVIGVGNIAEKAYLPTYAKKQGTLDFYFATRNKETKERIQK
                     EYGFQHLYETIDELLEEKIEACMIHAATKVHYELAKKCLENGIHVYIDKPLSVHLNEV
                     RELQELADKNQIILMIGFNRRFAPMVEELKRIPDKRLIHLQKNRIAAKETTEFVLYDL
                     FLHLIDTAVYLLDEPILRTKYQIRETKEFLEYAILQLETQNQTAILTMDLASGANIEK
                     YQVTSKNGTYEVNDLTQLVIQDADGKKVKEFGDWTNTLVKRGFEPMVEAFFSQIRLGK
                     SETELLKQKEVVRSHELCEEVLKDHYRHQL"
     gene            2078..3013
                     /gene="murB"
                     /locus_tag="IXT20_002666"
     CDS             2078..3013
                     /gene="murB"
                     /locus_tag="IXT20_002666"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HAQ4861071.1"
                     /translation="MNKEEIVESLATITILFDEPLMNYTFTKTGGPADVLAFPKKQEE
                     VKQIIDYCRIHDIPWMVLGNASNLIVQDGGIRGVVIMLTEMKQIHVKGTMVIAEAGAS
                     LIDTTYAALAESLTGFEFACGIPGSVGGAVYMNAGAYGGEIKDVFAEVDLLLEDGTLK
                     TLTKDEMVFSYRHSKVQELRAIVLEARFSLQTGDYEAIKARMDELTELRQSKQPLEYP
                     SCGSVFKRPVGHYTGQLIQQAGLQGLKWGGAQVSEKHAGFIVNIDHATATDYIELIAH
                     IQEVIKEKFDVSLETEVRIIGEKQAISETSRSLAN"
     gene            3311..4789
                     /locus_tag="IXT20_002667"
     CDS             3311..4789
                     /locus_tag="IXT20_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HAQ4861072.1"
                     /translation="MKLDYLIEEEDREKLLLARYLEFEKKNYVVLPKLYETLGLSKFK
                     TRNYIIELNKEFTEFKTKPEIQISEEAEIHVERLNLTDIKNLRRIYFQRSKIARLLTE
                     IVDSKVTIEKFAENHFLSISRAYVKRRELVSFLEQHQIKLKKNILVGEEINIRNVLFG
                     VYFGVFNGFSMPFRESVMNEAKSIVQYFEYLFNLKLSATKRIKFNLLTTISLLRAYGN
                     QPIGKRFFSDEYFKSSEGMKIIKDLSHISFLHIPKEQIIDEISYIMLFLITEEEFELD
                     GAFGLAEAHFHDIHMISQKMSVELIDQLPFSIEISEEERLYIRKELRMGMMHVNRKNW
                     IFDFEMTSFTSKKQLQFFFETYPIFSTAIQKVIQENKRYFLGKDERFMIRLFYDYLFL
                     LVGICPVSYFEAPIHVCIDFSQGSAYTDYIISQVKGFKNYNIVIESRITSRTNIYLSD
                     CLLEKFNKEQIIWKNPPTPDDWEYFGNTIIREKNRHLTEKIS"
BASE COUNT         1707 a          780 c          937 g         1437 t
ORIGIN      
        1 atctgcggta ataagcccaa ccaaacgaaa aatatgagga actatttttg tttggctgtt
       61 cttattactt gatgcaggac agctttttca caatctctcg ataaacagtg ttcaaaagcc
      121 agtttctcgg tattatgcca aatttatgca aaatgtggaa agccattttc acaaaatttt
      181 tcttaatact cgaaactaaa cgactttttc acaacctctt tatttcaaat ctaattctac
      241 tggacaatga tcactgccca taatgtccgt atggatcttt gcatcagcta gtcctggctc
      301 taaatctttg ctcgtcaaaa agtaatcgat ccgccaacca gcattgtttt tccttgaatt
      361 gaatcggtag ttccaccaag agtagacacc ttcctgggtt ggataaaaat aacggaatgt
      421 atcgataaac ccgttatcca acagtcgaga aagagccgct ctttcttctg gagtgaatcc
      481 agcatttttt tgattcgttt tccagttttt caaatcaatc ttttgatgag cgacatttag
      541 atccccacac acgatgactg gtttttgttt ttttagattt tccaaataat tataaaaggc
      601 ttcttcccat tctaatcggt aatctaaacg ttttaattcg ctttgagaat ttggcgtgta
      661 acaggttaca agaaagaaat cggaatattc caaagtaatc agacggcctt ctgtatcatg
      721 gatatcgata cccattccgt aagtggcatt caatgctggt tttttagcaa aaatcgctgt
      781 gccggaatag ccttttcgtt ccgcgtaatt ccaatattga taataacctg gaagatctaa
      841 atcaatttgt cctgcttgga gtttcgtttc ctgcagacag aaaaaatcgg cgtctaactc
      901 atgaaaaact tctaaaaaat ttttatttac gatggcacga agcccgttta cgttccaaga
      961 gataaatttc aataaaagca cactccttcg aattaatttc cttacctttg cgatactatt
     1021 gtacctcatt ttagtaaaaa atggtgtaag atgaaagtaa ctaaatgatc caggaggaaa
     1081 gtagatgaag attggtgtaa tcggtgtagg gaatatcgca gaaaaagcat atttaccaac
     1141 ttatgcgaaa aaacaaggaa cacttgattt ttattttgca acaagaaata aagaaacaaa
     1201 agaaagaatt caaaaagagt acgggttcca acatctatat gaaacgatcg atgagctttt
     1261 agaagaaaaa atcgaagcat gtatgatcca cgcagcaaca aaagtacatt atgaattagc
     1321 caaaaaatgt ctagaaaatg gtatccatgt ttatattgac aaaccactga gtgtccatct
     1381 aaatgaagtt cgtgaattgc aagaattagc tgataagaac caaataattt tgatgatcgg
     1441 tttcaaccgg cgctttgctc ctatggtaga agaattgaaa cgtatcccag ataagcggtt
     1501 gatccacctg caaaaaaatc gaatagcggc aaaagaaacg acagagttcg tcctttatga
     1561 tttgttttta catcttattg atacagctgt gtatttatta gatgaaccca ttttgagaac
     1621 aaaataccag ataagagaaa caaaggaatt tttggaatat gccattttac aattagaaac
     1681 acaaaatcaa acggctattt taacgatgga tttagcaagt ggcgctaata tagaaaaata
     1741 tcaagtaaca agtaaaaatg gtacttatga agtaaacgat ctgacccagc ttgtcattca
     1801 agatgctgat ggaaaaaaag tgaaagaatt tggcgactgg acaaacacgc tagtcaaacg
     1861 cgggtttgag ccaatggtag aggccttctt ttctcagatc cgtttaggta aatcggaaac
     1921 agaattgctt aaacaaaaag aagttgtaag aagtcatgaa ttatgtgagg aagtgcttaa
     1981 agatcactac cgtcatcaac tttgaacaag actggcaaga ttgtattaag cgtgatagaa
     2041 taatgaggac gtttgagaaa agaaaggaaa atcctcagtg aataaagaag aaatagttga
     2101 aagcttagcc acgattacta tattgtttga tgaaccattg atgaactata cgtttacgaa
     2161 aacaggtgga ccagctgatg tccttgcatt cccgaaaaaa caagaagaag taaagcaaat
     2221 catcgattat tgccgaatcc atgatatacc ttggatggta ttaggaaatg caagcaatct
     2281 gatcgttcaa gatggcggga ttcgcggtgt agtcatcatg ctgactgaaa tgaaacagat
     2341 tcatgtcaaa ggaacgatgg tcattgcgga agcaggtgcc tcactgattg atacgactta
     2401 tgcagctttg gcagaatcat tgactggatt tgaatttgct tgcggcatcc cgggaagtgt
     2461 cggtggagcg gtttatatga atgcaggtgc gtatggcgga gagatcaaag atgtttttgc
     2521 tgaagtggat cttttgcttg aagatggaac attgaaaact ttgacaaaag acgaaatggt
     2581 attttcttat cgtcacagta aagtccaaga attacgtgct atcgtattgg aagctcgatt
     2641 cagtttgcaa acaggggatt atgaagcaat caaagcaaga atggatgagt tgaccgaatt
     2701 gcgtcaatca aaacagccat tagaataccc ttcatgcgga agtgtcttta agcgcccagt
     2761 aggacactat acaggtcagc tgatccaaca agcaggattg caaggcctga aatggggcgg
     2821 tgcacaagtc tctgaaaaac atgctggatt catcgtcaat atcgatcatg cgacagcgac
     2881 agattatatt gaattgattg cgcacatcca agaagtaatc aaagaaaaat ttgatgtgtc
     2941 tttagaaaca gaagtgcgga tcattggaga aaaacaagca atatcagaaa caagcagaag
     3001 tttagccaat taataagtaa tggaacttaa aataagtaaa agagcaggac ggtggccgtc
     3061 ctgctctttt ttgtggttgt ttacgcaaat ggcgcaaatc ttgtacattt taacacaata
     3121 aaacaaaaaa tatagaatat ttcctcataa atttttccat tttttgcaat attctcaaaa
     3181 aaaagataaa aaatgagaat aactgatgaa aaaacaagca taatccatca taaaatcagt
     3241 ataggtgaaa agatgaactt tcatctgcat atcaagtaat tggtatgagg cattttttga
     3301 ggtgtaggat atgaagttgg attaccttat agaagaggaa gatagagaaa aactgctgct
     3361 agctagatat ttagaatttg agaaaaagaa ttatgtagtg ctgcctaagt tatatgaaac
     3421 attaggtttg tctaaattta aaacaagaaa ttatatcata gaattaaata aagaatttac
     3481 tgaattcaaa acaaagccag agatccaaat ttcagaagaa gcagagatcc atgtcgaacg
     3541 tctgaatttg acggatatca aaaatttaag aagaatctac tttcaacgat caaaaattgc
     3601 tcgactgtta acggagatcg tggattcaaa agtaacgatc gaaaaatttg cagagaatca
     3661 ttttttaagc atatcaagag cctatgtaaa aagaagagaa ttagtctctt ttcttgaaca
     3721 gcatcaaata aagttaaaga aaaacatatt agttggggaa gaaataaaca tacggaacgt
     3781 attatttggg gtatatttcg gggtctttaa tggtttttct atgccattta gagaaagcgt
     3841 catgaatgaa gcaaagagta tcgtgcaata ttttgaatat ctgttcaatt taaaactgtc
     3901 tgcaacaaaa cgaatcaaat tcaatttgct aaccactatt agtttgttga gggcctatgg
     3961 gaatcaacca atcggaaaac gtttcttttc agatgaatac tttaagagct cagaaggaat
     4021 gaagatcatc aaggatttat cacatatttc ttttcttcat atccctaaag agcagatcat
     4081 tgatgagatt tcttatatca tgttgttttt gatcacggaa gaagaatttg aattagatgg
     4141 cgcatttgga cttgcggaag ctcattttca tgatatccac atgattagtc aaaaaatgtc
     4201 agtggaattg attgatcaat taccattttc gattgaaatc tctgaagaag aaagactata
     4261 tatacgaaaa gaacttcgta tgggtatgat gcatgtcaat cgaaaaaatt ggatattcga
     4321 ctttgagatg actagcttta cgtctaaaaa gcagctacag tttttcttcg agacgtatcc
     4381 aatattcagt acagcaatcc aaaaggtcat acaagagaat aaacgctatt tcttgggaaa
     4441 agatgaacgt ttcatgattc gcttatttta tgattatctc tttttattgg tcggtatttg
     4501 tccggtatca tattttgaag caccgattca tgtctgtatt gatttttcgc aaggatctgc
     4561 ttataccgat tacattattt ctcaagtgaa aggatttaaa aattacaata ttgtgattga
     4621 gagtcgaatc acttctcgta cgaatattta tttatctgat tgtttacttg aaaaatttaa
     4681 taaagaacag ataatttgga aaaatccacc gactccagat gactgggaat attttggaaa
     4741 taccatcatc agagaaaaaa atagacattt aactgaaaaa atcagctagt atttatggat
     4801 ggcagctttg tctcacatat ttattagctg atttatgttt ttttcaaaga aaaggtaact
     4861 a
//
LOCUS       DACGLE010000121         4835 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0534
            SAMEA3304449-rid9560453.denovo.128, whole genome shotgun sequence.
ACCESSION   DACGLE010000121 DACGLE010000000
VERSION     DACGLE010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304449
            Sequence Read Archive: ERR985064
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4835)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4835)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702225.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702225.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 20:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,045
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 27 of 67
            Pseudo Genes (incomplete)         :: 25 of 67
            Pseudo Genes (internal stop)      :: 19 of 67
            Pseudo Genes (multiple problems)  :: 4 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4835
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304449-rid9560453.denovo.128"
                     /strain="VREN0534"
                     /db_xref="taxon:1352"
     gene            complement(<1..556)
                     /locus_tag="IXT20_002668"
     CDS             complement(<1..556)
                     /locus_tag="IXT20_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016182552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HAQ4861073.1"
                     /translation="MAIEAGLVLFFVFVVLLVLGMPIAISIAFSSMMTLLLVIPFDVS
                     AFSSAQKMVGSINSFSLIAIPFFVLSGIIMNNGGIAKKLVDFAMLFVGRVPGALAHTN
                     VLGNALFGSISSSAIAASTAIGGVLIPQQVEEGYDRKFATAVNIASAPTGMVIPPSTA
                     FIMYSLVAGGASISALFMGGYLVGA"
     gene            complement(572..1051)
                     /locus_tag="IXT20_002669"
     CDS             complement(572..1051)
                     /locus_tag="IXT20_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HAQ4861074.1"
                     /translation="MKMILNKVLEILGAVLLVGMVAVVLWQVFSRTVLGNPNTVTEEL
                     VRFGLVWFAMLSSAYVVGQKARLAVTVLSEKLTGKKSKILEVVVQSLFLVFAAMIMVY
                     GGWNAVTLTMGQISPSLSIPMGYVYLSVPVSGVLIIIYSLINLIDTLSNKPAIQEKI"
     gene            complement(1057..2081)
                     /locus_tag="IXT20_002670"
                     /pseudo
     CDS             complement(1057..2081)
                     /locus_tag="IXT20_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294179.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
     gene            complement(2098..3204)
                     /locus_tag="IXT20_002671"
     CDS             complement(2098..3204)
                     /locus_tag="IXT20_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HAQ4861075.1"
                     /translation="MIIKEEIFVPKELVKEKIDLLVENLTTIKDDNGEFLLDFDGLKV
                     DDKSWTVWNWPQGVGLYGIYKNYQMTKNPRAYQVVNEWFEDRMEEGAPPKNVNTMAPL
                     LTMAYLYEDTKDSRYLPYLEQWAEWVMNDMPRTNEDGLQHATYGPENKNQLWDDTLMM
                     TVLPLAKIGKLLNRPEYLEEARYQFMIHIKYLMDKRTGLWYHGWTFEGNHNYAEAFWA
                     RGNCWLTIAIPEIIEILELSKEDALRKLLIETLEAQVRALKTYQSESGLWHTLLDDPS
                     SYVESSATAGFAYGILKAVHKRYLPQEYKEVAYRAIQGLLEQIDEKGEVQNVSIGTGM
                     GDSLDFYRNIGITAMPYGQSLTVLCLTELLVSYC"
     gene            3510..4802
                     /locus_tag="IXT20_002672"
     CDS             3510..4802
                     /locus_tag="IXT20_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HAQ4861076.1"
                     /translation="MSYTHLIKETLDILDLSVTFNENCLTKEKYKGQICHIYRGNLIY
                     TAQECIHCKHQIASDIVRWGTTTVRLLMNDVSECRTYLELKKQRFKCKACQRTFVADT
                     SVAKKHCFISEKVRWSVVTRLKKNTSMTEIAAQKNLSVSSVYRIMKRFYRPLNPFRTP
                     LPKVLCFDEFKSVRGVSGAMSFIMMDGQTQRLLDIVENRQLPFLKRYFSHFSREIREA
                     VEWIVIDMYAPYVSLVKKLFPKAQLIIDRFHIVQHIGRTFRNHRIKETNQLLKSKEQK
                     HYQLGKQLKRYWKLLQKDERKLDYTRRLWRPGFKAHLTETDIVDRLLKGSPALRVGYQ
                     LYQDFLYAVKERDYVSFEELLTNNIMLPEGYQTTLRTFQKFLPQIKNALQQSYSNGPL
                     ECLNNHIKVLKRNAYGFRSFYNFKLRIMIRHGNALIFN"
BASE COUNT         1469 a         1083 c          833 g         1450 t
ORIGIN      
        1 aagcgcctac gagataacct cccataaaca aagcagagat cgaagcacca ccagcaacaa
       61 gtgaatacat gataaaagca gtacttggag gaatgaccat acctgtcgga gctgacgcga
      121 tattgaccgc tgtcgcaaac ttccggtcat atccttcttc cacttgctga gggatcaaca
      181 ctccaccaat cgcggtagaa gcggcaatgg ccgagctaga gatggaacca aataaagcat
      241 tccctaacac attcgtatga gccaaagccc ccggcacgcg cccgacgaac aacatcgcaa
      301 aatccactag tttcttagct atccctccat tattcatgat aatgcccgag agaacgaaaa
      361 acggaatagc gatcagcgaa aaactattga tacttcccac cattttttga gctgaactaa
      421 aagcagaaac atcaaacggt ataactaaca ataaagtcat catcgatgag aatgcaatac
      481 ttatagcaat cggcatccct aatacaagca acacaacaaa tacaaaaaat aacactaatc
      541 cggcttctat tgccatttta ttcctccatt tttatatttt ttcttgaatt gctggcttgt
      601 tgcttaatgt atcgattaga ttgatcaggc tatagataat gatgagtacc cccgatacag
      661 gtactgacag ataaacatat cccataggaa tacttaaaga aggcgagatc tgccccattg
      721 tcaatgtgac agcattccat ccgccataca ccatgatcat cgcagcaaat accagaaata
      781 aactttgaac aacaacttct aaaattttac tttttttgcc agttaatttt tcacttaaaa
      841 ccgttacggc taaacgtgct ttttgtccca caacatacgc ggaagacaac atggcaaacc
      901 agacaagacc gaatcgaaca agctcttccg tgaccgtatt tggatttcct aaaaccgttc
      961 tggaaaatac ttgccataaa acaacagcga ccatcccaac aagcaaaaca gctcccagga
     1021 tctctaatac cttgtttaaa atcattttca ttacagctac gctccttgct cttggatctt
     1081 ttgataaagg tctttgtagg taggatcgtt tttgaatata tcatgaagtg gggcaactgc
     1141 ctctcgaaac ggtgtattat ctacatcatt gaattggact ccatgttctt cttgtgctaa
     1201 agctttttct tcttcaattg cttttgcaaa agcttcaatt tcaaaaaccg ttgattcttt
     1261 cgctgcttct tcaatggctt tttcctgttc atcagttagc tgttctttga tggcatcatt
     1321 catgatgatg atatctggca cacgtgtatg ctcatcatag gaatagtatt tagctacccc
     1381 gccatgtcca gctgtataca agacaaattc attgttttct gatccgttga tcaaattgga
     1441 ttgaagagac gtgtacactt catcacttcc cataggtacg ggcgaaccgc ctaaaagctc
     1501 taccattttg atagcggttt cgctttgcat cacacggatc ttctttccct tcaaatcttc
     1561 cggcgtatgg atcggaccgt ctaccatata aaagctgcgc tgaccagaat cataataggt
     1621 caatccggta aatcctaaat ttttggtcga ttggtagatt ggctccatta tttcttcatt
     1681 atccatcacc ttaaagaaat gttcttcatc attgaataga taaggaacgg aaaaaatcga
     1741 ataatcttta gagaaacttt ccaacgctga ggcacttact tttgtgaaat ctactgctcc
     1801 tgactgtgtc aattcgatca gttcacgttc tccacccagt tgtccatctg ggaaatactg
     1861 gatctgtacg gaaccatctg ttttttcttt tactaaacgc ccaaattcat tcattgcatc
     1921 aattactggc tgttgcttgc ataagcaaag cgcaaggtaa ctatctcatt tacgtctgca
     1981 tctgcgacag ttgccacact agaattattt ccgcagccgc ttagacctag cagacctagg
     2041 ctaagcgaac ttgctgcaaa cagcagtgat gtttttttca tatgggtttc ctttctttta
     2101 acaataagag acgagcaatt ctgtcaaaca aagcaccgtt aacgactgtc cataaggcat
     2161 tgctgtgatt ccgatattcc gataaaaatc taaactgtcc cccattcctg tcccgattga
     2221 aacgttttga acttctcctt tttcatcgat ttgttcaagc aatccttgga tagcacgata
     2281 agcgacttct ttatattctt gtggtaaata acgtttatgg accgctttca aaataccgta
     2341 agcaaatcct gcagttgcag aagattctac gtaagaagaa ggatcatcca acaacgtatg
     2401 ccataaaccg ctttcagact gataagtttt taatgcacgc acttgtgctt caagtgtttc
     2461 gatcaataat ttgcgcaatg catcttcttt cgataattct aagatttcaa tgatctctgg
     2521 aatagcaatc gtcaaccagc agtttccacg agcccaaaat gcttcggcat agttatgatt
     2581 tccttcaaac gtccagccgt gataccacaa tcctgttcgt ttatccatca agtatttgat
     2641 atggatcata aactggtaac gagcttcttc taaatattct gggcgattca acagtttccc
     2701 gatttttgcc aaaggaagca ctgtcatcat caatgtatcg tcccacaact gattcttatt
     2761 ttccggtcca tatgtggcat gctgtaaccc gtcttcattt gtccgaggca tgtcattcat
     2821 gacccattct gcccattgtt ctaaataagg caaatagcgt gaatctttcg tgtcttcata
     2881 caagtaagcc atagtcaata gcggtgccat tgtattgacg tttttgggcg gagcgccttc
     2941 ttccatacga tcctcaaacc attcattgac cacttgataa gcgcgaggat tttttgtcat
     3001 ctggtaattt ttataaatac catatagacc gacaccttgt ggccaattcc acactgtcca
     3061 gcttttgtca tctactttca gtccatcaaa gtccaacaaa aattcaccat tatcgtcttt
     3121 gatcgttgtc aggttttcaa cgagcaggtc aattttttct ttcactaatt ctttaggtac
     3181 aaaaatctct tctttaataa tcatctttac gccacctttt taaacgcttt cattttatat
     3241 ccttaatata gcagtaatac cgtccactat aaatatcgct ttttgaacaa aaactgtcta
     3301 aaattgacct aaatgttaaa taacaaaagt aactttcagt tacttggaaa ttaaaaaaag
     3361 aacttggaga aacaacgttt tccagctcta tactttttga tgttggtgga tgacaccagc
     3421 atctttttta ttttccaaaa catatagaaa agacaccttg aaatccttta gaattaattt
     3481 ggcgaaccaa cccaaaagga ggaatcaaga tgtcttacac tcaccttatc aaagaaacat
     3541 tagatattct agacttatca gttactttta acgaaaattg tctcacaaaa gaaaaataca
     3601 aagggcaaat ctgtcatatt tatcgtggaa acttgattta tacagcccaa gaatgcatcc
     3661 actgtaaaca ccagattgct tcagatattg tgcgttgggg tacaacaacc gtccggttgt
     3721 taatgaacga tgtttctgaa tgccgtacct acctcgaatt gaaaaagcaa cgcttcaaat
     3781 gcaaagcttg tcagcggacc tttgtcgcag atacttctgt tgcgaaaaaa cactgtttca
     3841 tcagtgagaa agtccgctgg tctgtcgtga ctcgtctgaa aaaaaatacc tcaatgacag
     3901 agatcgccgc tcagaaaaat ctatcggtct cttctgttta ccgtattatg aagcgatttt
     3961 atcgtccgtt gaatcctttt cggacaccgt tacctaaggt actttgtttt gatgaattca
     4021 aatcagttcg gggtgtttca ggtgctatga gtttcatcat gatggatgga caaacacagc
     4081 gcttgttgga cattgtcgag aaccgacaac tgcctttttt aaaacgttat tttagtcatt
     4141 tttcacgaga aattcgtgaa gcggtcgaat ggatcgtgat tgatatgtac gctccttatg
     4201 tatcgctcgt taaaaagctg tttccaaaag cacagctgat cattgaccgg tttcacatcg
     4261 tccaacatat tggtcgtacc tttcgcaatc atcgaatcaa agaaacgaac caactattaa
     4321 aaagcaaaga acaaaaacac tatcaattag gtaagcaatt aaaaagatac tggaaacttc
     4381 ttcaaaaaga tgaacgcaag cttgattata ctcgacgcct ttggcgacct ggcttcaaag
     4441 ctcatttaac agagaccgat attgtggatc gcttactgaa agggagccct gctttgcgtg
     4501 taggctatca gttatatcaa gactttcttt acgctgtaaa ggaacgagac tacgtatcgt
     4561 ttgaagaact acttaccaat aatatcatgc ttcccgaagg ctatcagacg accttgagaa
     4621 cgttccagaa atttctgcca caaatcaaaa atgctttaca acaaagttat tcaaatggcc
     4681 cacttgaatg tctgaacaac catatcaaag tgttaaaacg aaatgcatat ggctttcgta
     4741 gcttttataa tttcaaattg cgcatcatga ttcgtcatgg caacgcattg attttcaact
     4801 aaaaaagatc aagaggaact gatccacttg atctc
//
LOCUS       DACGLE010000122         4782 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0534
            SAMEA3304449-rid9560453.denovo.129, whole genome shotgun sequence.
ACCESSION   DACGLE010000122 DACGLE010000000
VERSION     DACGLE010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304449
            Sequence Read Archive: ERR985064
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4782)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4782)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702225.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702225.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 20:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,045
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 27 of 67
            Pseudo Genes (incomplete)         :: 25 of 67
            Pseudo Genes (internal stop)      :: 19 of 67
            Pseudo Genes (multiple problems)  :: 4 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4782
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304449-rid9560453.denovo.129"
                     /strain="VREN0534"
                     /db_xref="taxon:1352"
     gene            complement(207..1169)
                     /locus_tag="IXT20_002673"
     CDS             complement(207..1169)
                     /locus_tag="IXT20_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018375965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAQ4861077.1"
                     /translation="MVAKLTDVAELAGVSPTTVSRVINNKGYLSEKTKQKVHEAMKTL
                     GYKPNNLARGLQGKSPQLIGLIFPNISNIFYSELIEYLEIELFKHGYKTIICNSQNDP
                     AKEREYIEMLEANQVDGIISSSHNLGIADYERVRAPIVAFDRNLAPNVSIVSSDNFEG
                     GKLAAKTLQKNGCQNTIMITGNDNTDSPTELRALGFSFQNPNGKIFKIPNNLSTIRRE
                     MEIKSTIINHKPDGIFVSDDLTAILTMKIAHQLKLNIPEDLKIIGYDGTSFIEKFFPQ
                     LTTIRQPIDEIASLIVDILLKKIKGEKTNKDYILPISILSGGSI"
     gene            complement(1171..2610)
                     /locus_tag="IXT20_002674"
     CDS             complement(1171..2610)
                     /locus_tag="IXT20_002674"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HAQ4861078.1"
                     /translation="MNLPREIRYRAYSDWTKDEMDKIKDNVKRSPWRASYHIEPKTGL
                     LNDPNGFSFFNGKYTLFYQNWPFGAAHGLKEWVHNESDDLVHFHVTGAELLPDTKRDS
                     HGAYSGSAYEIEGNLFLLYTGNVRNENWERDTLQIGAWMDKDYNITKGENVLIHKPAD
                     VTDHFRDPQMFNYEGQLYAIVGAQNLEKSGFIKLYKADDNNVETWEEVGNLDFGGTGS
                     EYMIECPNLVFVDKKPVLIYCPQGLDKSELNYGNIYPNTFKICQSLDIEKVKLVGASE
                     IQNLDYGFEAYATQGFNTPDGRALIVSWIGLPDIDYPTDKYDYQGAMSLVKELSIKDG
                     KLYQYPVEAITSLRASSEQFTAKAETNNTYELELTFPPNQKSELLLFSDSKGNGLSLI
                     VDTKEGKIILDRSKAGIQYATDFGTIRECSIDTKETTANIFVDNSIIEIFINKGEKVF
                     TSRVFPENHQNGIQIKSGEPTGKYFELKY"
     gene            2795..4741
                     /locus_tag="IXT20_002675"
     CDS             2795..4741
                     /locus_tag="IXT20_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS beta-glucoside transporter subunit IIBCA"
                     /protein_id="HAQ4861079.1"
                     /translation="MNYSKVASDVIQAVGKDNLIAAAHCATRLRLVLKDDTKVNQKAL
                     DENPDVKSTFKIDGQYQVIIGAGDVNFVYDELIKKTGLSEISTDDLKQIASKDKKFNP
                     IMALIKLLSDIFVPIIPALVAGGLLMALQNFLTAAGLFGPKSIEEMYPAIEGISSMIQ
                     LMSAAPFMFLPILVGISAAKRFGANQFLGAAIGMIMTTPDLGGSTEYWNIFGYHVSQT
                     NYAYQVIPVLAAVWILSILEKFFHKKLPSSIDFTFTPLLSVMITGFLTFTVIGPVMLL
                     LSNGITDIIVWLYNTTGFIGMGIFGGTYSLIVMTGLHQSFPAIETQLLSAWTNGIGHG
                     DFIFVVASMANVAQGAATFAIWFLTKNSKTKSLASSAGVSALLGITEPALFGVNLKYR
                     FPFFCALIGSGIATAVAGLLKVVAVSLGSAGFLGFLSINATSIPFYVLCELISFVVAF
                     AITYFYGKTKAAGIFKAEEIAEQTPVNSSKVATTDPISTDVESTQTKVSSETIVSPIS
                     GKVISLSSVNDPVFSSEAMGKGIAIKPNGNTVHSPVDGTIQLTFETGHAYGIKSNNGA
                     EVLIHIGIDTVSMGGKGFTQKVTTNQKVRKGDILGAFDRSMIEKEGLDDTTMIIVTNT
                     SDYSEVNIVAENTVTEGTDLLEIK"
BASE COUNT         1521 a          820 c          789 g         1652 t
ORIGIN      
        1 cgtccaattt cgtaaaacaa ccgtaacagt tgtgtaaaaa tatcatttta gagcatgaaa
       61 aaaggagtgg atttctccac tccaaaaaag tctaactttt gggggtcact acaagttgac
      121 aactcctttt tgtttacaaa tcaattggat atctacaaat tgtattttct aacgaatttt
      181 atagacatta aaaaattaat ttaacgttaa atacttcctc cagaaagtat tgagattggt
      241 aaaatataat ccttatttgt cttttcgcct ttaatttttt tcagaagaat atcaacaata
      301 agacttgcaa tttcatcaat tggttgtctt attgttgtca attgtgggaa aaatttctca
      361 ataaaagagg taccatcgta tccaataatt tttaaatctt ctgggatatt aagtttaagt
      421 tgatgagcaa ttttcatagt gagaatggct gttaaatcat cagaaacaaa aataccatct
      481 ggtttgtgat ttataatagt tgactttatt tccatttcac gacggatagt agacagatta
      541 ttaggaattt tgaaaatttt tccatttgga ttttgaaaag aaaaacctag tgcacgtaat
      601 tctgtgggag aatccgtgtt atcatttcct gtaatcataa tggtattttg acaaccattt
      661 ttttgtaaag tcttagctgc aagttttccc ccttcaaagt tatctgaaga aacaatggaa
      721 acattaggag ctaaatttct atcaaaagca acaataggtg ctctcacacg ctcataatca
      781 gcaattccaa gattatggct tgatgagatt atgccatcaa cttggttggc ttcaagcatt
      841 tcaatgtatt ctctctcttt agcaggatca ttttgactat tgcaaataat cgttttatat
      901 ccatgtttaa ataactctat ttcgagatac tcaatgagtt cagagtaaaa gatattacta
      961 atattaggaa aaataagacc aataagctga ggtgactttc cttgaagtcc acgtgctaaa
     1021 ttatttggtt tataaccaag agttttcata gcttcatgaa ctttttgttt tgttttttct
     1081 gataaatatc ccttattatt aatgacgcgt gaaacggttg ttgggctaac tccagctaat
     1141 tcagcgacat cagttaattt tgctaccatt ttaatatttt aattcaaagt attttccagt
     1201 aggttcgcct gatttgattt gaataccatt ttggtgattt tcagggaata cacgactagt
     1261 aaacactttt tctcctttat taataaaaat ctcaatgatt gagttatcta caaaaatgtt
     1321 agcagttgtt tctttagtat caattgagca ttcacgaatc gtaccaaagt cagtggcata
     1381 ttgaatgcct gctttgctgc gatctaaaat aatttttcct tctttggtat ctacaatcaa
     1441 gctaagtcca tttcctttac tatcagagaa aagaagtaac tccgatttct gatttggagg
     1501 aaaagttagt tcaagctcat aagtattatt tgtttctgct tttgctgtaa attgttcaga
     1561 actagcacgt aatgatgtaa tagcttctac tggatattgg taaagtttac catcttttat
     1621 tgaaagttct tttacaagac tcattgctcc ctgataatca tacttatcag ttggataatc
     1681 gatatcaggt agtccaatcc aactgacaat cagagcacga ccatcaggag tattgaatcc
     1741 ttgagtcgca tacgcttcaa atccataatc aagattttga atttctgaag cgcccacaag
     1801 ttttactttt tctatgtcaa gcgattgaca gatcttaaaa gtattcggat agatattccc
     1861 ataatttagc tcagatttat ccaacccttg aggacaataa atgagaacag gttttttgtc
     1921 tacaaatact agatttggac actcaatcat gtattctgaa ccagtgccgc caaaatcaag
     1981 gttgccaact tcctcccatg tttccacgtt attgtcatcc gctttgtaaa gtttgataaa
     2041 gcctgatttt tctaaatttt gtgctccaac aatcgcatac aattgtcctt cataattaaa
     2101 catttgtgga tcacgaaaat gatcagttac atcagcaggt ttatgaataa gaacattctc
     2161 acctttcgta atgttgtagt ctttatccat ccatgctccg atttgaagtg tatcacgttc
     2221 ccaattttca tttcgaacgt ttcctgtata gagtaagaat agattcccct ctatttcata
     2281 tgcagaaccg gaataagcgc cgtggctatc tcgttttgtg tctggaagaa gttctgctcc
     2341 tgttacatga aaatgaacta aatcatctga ttcattgtgt acccattctt tcaacccatg
     2401 agcagctcca aatggccaat tttgataaaa aagcgtatat tttccgttaa aaaaagaaaa
     2461 accattagga tcgtttaaca atcctgtttt aggttcgata tgatagctag cacgccaagg
     2521 tgatcgtttt acattatcct taattttatc catctcatct ttagtccaat cagaataagc
     2581 acgatatcgt atttctcgag gtaagttcat attttactcc ttcaatttat ctttttacaa
     2641 ctagtatggt aaacgttttc cgttgaaaaa tcaatcattt catttaaaaa aataaactat
     2701 atgacaaacg tttgacatat ttttaagata tgctaaaata aatttgcatt tcagtgaacc
     2761 gttttcacgt aaaaataaaa aaggagtgat tcgaatgaat tacagtaaag tagcttccga
     2821 cgtaatacaa gcggtcggaa aagataacct tattgcagct gcacactgcg ctacacgtct
     2881 tcgtctcgta ctaaaagatg acacgaaagt gaatcaaaaa gctttagacg aaaatccaga
     2941 tgtgaaaagc acatttaaaa ttgatggaca atatcaagtg attatcggtg caggtgatgt
     3001 taatttcgtt tatgacgaac ttatcaaaaa aactggactt tcggaaattt caacagatga
     3061 tttaaaacaa attgctagta aagataaaaa attcaacccc attatggcat taattaaatt
     3121 attatcagat atttttgttc caattatacc tgcccttgtt gcaggtggtt tattaatggc
     3181 attacaaaac tttttaacag ctgctggact ttttggacca aaatcaattg aagaaatgta
     3241 tcctgcgatt gaaggtattt cctcaatgat ccaactgatg tccgctgcac cattcatgtt
     3301 cttaccaatt ttagtaggta tttcagctgc taaacgtttt ggagcaaacc aatttcttgg
     3361 tgctgcaatt ggtatgatca tgacaacacc agatcttggt ggctcaactg aatattggaa
     3421 tatttttggt tatcatgttt cacaaacaaa ctatgcttat caagttattc cagttttagc
     3481 agctgtttgg atattatcta tattagaaaa attctttcat aaaaaattgc catcatctat
     3541 tgactttaca ttcacgccat tattatcagt aatgattact ggatttttga ctttcacagt
     3601 tattggccct gtaatgttat tgttgtctaa tggaattaca gacattattg tttggctata
     3661 taacactact ggatttattg gaatgggtat ttttggagga acatattcac ttatagtcat
     3721 gactggttta catcaatcat ttccagctat tgaaactcaa ttactttcag cgtggacaaa
     3781 tggtatcgga catggagact ttatctttgt agttgcatct atggcaaacg ttgctcaagg
     3841 tgctgcaaca tttgccattt ggttcttaac taaaaattct aagacaaaaa gtcttgcttc
     3901 ctctgcgggt gtctcagcac ttcttggaat tacagagcct gccttgttcg gagttaactt
     3961 aaaataccgc tttccattct tttgtgcttt aattggttca ggaattgcta cagcagtagc
     4021 aggattactt aaagtagttg ctgtttcact tggttcagca ggtttcttgg gatttctttc
     4081 aattaatgca acatctattc cattttacgt attgtgtgaa ttgattagct ttgtagttgc
     4141 ttttgcaatt acttacttct acggtaaaac aaaagctgct ggtatcttta aagcagaaga
     4201 aattgctgaa caaacaccag ttaattcttc aaaagtagca acaacagatc caatatctac
     4261 tgatgttgaa tcaacacaaa caaaagtttc atcagaaaca attgttagtc ccatttctgg
     4321 taaagtgatt agtctttctt cagtaaatga tccagtcttt tcatcagaag ctatgggaaa
     4381 agggattgcc attaaaccaa atggaaacac ggtccattct ccagtagatg gtactattca
     4441 actcacgttt gaaactggac atgcttatgg tattaaatca aataatggtg ctgaagttct
     4501 aattcatatt ggtattgata ctgtatcaat gggaggaaaa ggatttacac aaaaagttac
     4561 tactaatcaa aaagttagaa aaggcgatat tcttggagca tttgaccgtt caatgattga
     4621 gaaagaaggt cttgatgata caacaatgat tattgtgacc aatacttcgg attattcaga
     4681 agtaaatata gtagctgaaa atacagtcac tgagggtact gatttacttg aaatcaaata
     4741 aatatatttc cctgagtaaa gatggaattt tctttccaaa at
//
LOCUS       DACGLE010000123         4773 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0534
            SAMEA3304449-rid9560453.denovo.130, whole genome shotgun sequence.
ACCESSION   DACGLE010000123 DACGLE010000000
VERSION     DACGLE010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304449
            Sequence Read Archive: ERR985064
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4773)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4773)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702225.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702225.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 20:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,045
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 27 of 67
            Pseudo Genes (incomplete)         :: 25 of 67
            Pseudo Genes (internal stop)      :: 19 of 67
            Pseudo Genes (multiple problems)  :: 4 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4773
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304449-rid9560453.denovo.130"
                     /strain="VREN0534"
                     /db_xref="taxon:1352"
     gene            complement(403..744)
                     /locus_tag="IXT20_002676"
     CDS             complement(403..744)
                     /locus_tag="IXT20_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HAQ4861080.1"
                     /translation="MKPNEIVTVYAAFTDKNGGKRRPILVVSDKEDRVEFFGITSQYE
                     NKSDQIKRVYFPIAEWEKVGLKKQSWIDVGSLKAIPKSRENISFKKVGMLSAIDTKKL
                     NRFIERLQRKV"
     gene            complement(734..1015)
                     /locus_tag="IXT20_002677"
     CDS             complement(734..1015)
                     /locus_tag="IXT20_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HAQ4861081.1"
                     /translation="MKVKNPKEKSRIQVGIDKDLKENAEMILEELGLNPTTAITILYK
                     QVVARGEFPVEIKLSEEEKQGIRLQQLTMDKPVDVLDTDEKLEEWFNEA"
     gene            complement(1122..1706)
                     /locus_tag="IXT20_002678"
     CDS             complement(1122..1706)
                     /locus_tag="IXT20_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4861082.1"
                     /translation="MNENKMVLSEYARLRTINWLNGMQSSGAFDGFDEENMDFESLSN
                     EDIRYYFIEWYFTEPDEEVEDEPELILERQRILFSEQDSKRLYGLYDLQLKGLKAQYV
                     ADSYDDMVEQGADRVVTYSEDDEEEWENYSDDEICFNDGYMPIEITEEEYDKVSDSDE
                     IGMCTVVSGLDIDVQRVEVAYQKMKERIKSKVES"
     gene            complement(1723..1962)
                     /locus_tag="IXT20_002679"
     CDS             complement(1723..1962)
                     /locus_tag="IXT20_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4861083.1"
                     /translation="MTKFKSKGMQWCSYYYVGNDEELLRKAKQKGYPDKMLKQIGYYM
                     PEERIEKESCKVLFDSEQLLVLLTQNKLICQFENN"
     gene            complement(1981..2229)
                     /locus_tag="IXT20_002680"
     CDS             complement(1981..2229)
                     /locus_tag="IXT20_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4861084.1"
                     /translation="MEGMDFIDHEDLIDFGYTWKGMVGISRSLANAFYERNYAVYVLY
                     DDNTESLVDEEYKLDLENVLYGIEKEDLAKYIFSWLGQ"
     gene            complement(2244..2810)
                     /locus_tag="IXT20_002681"
     CDS             complement(2244..2810)
                     /locus_tag="IXT20_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4861085.1"
                     /translation="MFEKRKDQFPNLEAKSQKVIDGEMIELFQKSYGTIETTASCKYY
                     FQGKRNTFKGFADSDEELAKKIIDWLVSSEHVAREDTVRTTIEGEVLTGTRCVCCNHE
                     FVEDEGCYRINEYGDFICQSCHEIEFPTERSWFEYMLQDYEMETENGEVVFCTKEELS
                     KRSDDEIEDWCVWLVGTDDCVAYYTTAE"
     gene            complement(2811..3356)
                     /locus_tag="IXT20_002682"
     CDS             complement(2811..3356)
                     /locus_tag="IXT20_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4861086.1"
                     /translation="MGERTQALITVKYGNSKTKVSVSIHYQWGYGRFMLMDVLNQGIY
                     LINKAYDLRDESREELAKGIFDRSTGVINFVTYESGVSAYDETGFGGRYEPYQETIED
                     VMKFSDNNDGYAHLEMVFDENGFSLDDKTRLRLFDFEGQQIGLNKFCESGKEFATKDF
                     IKGYRLLLENYGINLEEESVK"
     gene            complement(3382..4149)
                     /locus_tag="IXT20_002683"
     CDS             complement(3382..4149)
                     /locus_tag="IXT20_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="JAB domain-containing protein"
                     /protein_id="HAQ4861087.1"
                     /translation="MRDRILEVNQEMVNKLTNISGNYEKYDGTLLDNYIFHDTQNIRI
                     GRSKPRAFILIKEKYLNEWNSTYEMVMTDNFEKVLDFRNALLGSDMGNSSLESIEVEN
                     KTENEGEGLIKNVLKIEQKYISEDRSFYRVTSSVGLANFATNLIGNNASESLLVMCLN
                     TKNEVIAYSEVFKGGINQSVAIPREIFQLALLNNSAKIAVSHNHPSGYEFPSENDKSF
                     TTNLKNAGKTIGIELLDHIIVTSKNTSYYSFQENGSL"
BASE COUNT         1352 a         1028 c          587 g         1806 t
ORIGIN      
        1 tttctattca agtgggtaac gttcgtttgc aagcaaactc tctctatgta acttgccagt
       61 tacatcagtg gtaacgctac gtcgcaagcg acttgctcta atgaaactac cgtttcattc
      121 tgcctgttcg ttcgtagcaa gctacttcac tcactgtatc cgtctgaatt attgttattt
      181 attactattc aatatccgtc agtcatttta tttatttaca tacattaaat ataaaaatgt
      241 atcttataat tcgtatatta ctaattatca ctatttaaca atacttaata ttttcttaat
      301 ttttataatc acgtatatat ttacgtgtat tttaatatac ataaatacgt ttatataaac
      361 gtatatctta atatacatat aaacaaaaaa agaaaagcct tgttagactt ttctttgtaa
      421 tctctcgata aacctattta gctttttcgt atcaatagct gatagcatac caaccttttt
      481 aaatgatatg ttctctcggc ttttgggaat agcctttaaa gaccctacgt caatccatga
      541 ttgcttcttt agacctactt tttcccattc tgcaatagga aaatacactc tctttatttg
      601 gtctgattta ttctcatatt gacttgtgat tccaaaaaat tctactctat cttctttatc
      661 actaaccacc aaaataggtc ttcttttacc accattttta tctgtaaatg cagcatagac
      721 cgttactatc tcattaggct tcattgaacc actcttctaa cttttcatct gtatctagaa
      781 catcaactgg cttatccata gttagctgtt gcaatctgat tccttgcttt tcctcttcgc
      841 ttagctttat ctctactgga aactcacctc tagcgaccac ttgcttataa aggattgtga
      901 ttgctgttgt tggatttaac cctagctctt ccaatatcat ttctgcgttt tcttttaaat
      961 ccttgtctat tccgacttga attctgcttt tttcttttgg attttttacc ttcatttcgt
     1021 tcacctcttt tgagtataat atactcatta ttatatccat taactacatt tatgattata
     1081 ttatactctt aattagctac tttgcaaaca atccaatcaa atcaagattc tactttactt
     1141 tttatacgtt ctttcatctt ctggtatgca acctctactc tttggacatc tatatccaat
     1201 ccacttacaa ccgtacacat accaatctca tcgctatccg acactttatc atattcttct
     1261 tcggtgattt caataggcat atacccatca ttaaagcata tctcatcatc tgaatagttt
     1321 tcccattcct cttcatcatc ttctgaataa gtgacaaccc tatctgcccc ctgttctacc
     1381 atatcatcgt aactatcagc aacatactgc gcttttaatc ctttcagttg taagtcatat
     1441 aaaccataca acctcttact atcttgttca ctgaataaaa ttctttgacg ttctaaaatt
     1501 aattctggtt catcttccac ctcttcatct ggttctgtaa aataccattc gataaaataa
     1561 tatctaatat cctcattcga taaactttca aagtccatat tctcctcatc aaatccatca
     1621 aaagcgccac tactttgcat accattcaac caattaatgg ttctcaatct tgcgtattca
     1681 cttaatacca tcttattctc attcatggtt cttcacccca tattaattat tttcaaattg
     1741 acaaatcaat ttattttgag ttaacaaaac taatagctgt tcactatcaa aaagaacctt
     1801 acaagattct ttttctattc tctcttctgg catgtaatag ccaatctgtt ttaacatctt
     1861 atctggataa cctttttgtt tggcttttct caaaagctct tcatcattcc caacataata
     1921 ataagaacac cattgcatac ctttactttt gaattttgtc atgttgctac cctctttcgt
     1981 ttattgaccc aaccatgaaa aaatatattt tgctaaatct tctttttcta tcccatataa
     2041 aacattctct aaatccaatt tatactcttc atcaacaagg ctttctgtat tgtcatcata
     2101 aaggacatag actgcataat ttctttcgta aaaggcatta gcaagacttc tgctaatacc
     2161 taccattcct ttccatgtat aaccaaaatc aatcaaatct tcatgatcta taaaatccat
     2221 tccttccatg ctgattcaca ctcctattct gctgttgtgt aatatgcaac gcaatcatct
     2281 gtccctacta accacacaca ccaatcttca atttcatcat cgcttctctt actcaattcc
     2341 tctttggtac aaaaaactac ttcaccattt tctgtttcca tctcataatc ttgtagcatg
     2401 tattcaaacc aacttctttc ggttggaaat tctatctcgt ggcaactctg gcaaataaaa
     2461 tcaccatatt cattgattcg gtagcaacct tcatcttcta caaattcatg attacaacaa
     2521 acacatctag tacctgttag gacttcaccc tcaatcgttg ttctaacggt atcttctctt
     2581 gctacgtgtt cactgctgac aagccaatca atgattttct ttgccaattc ttcatcagaa
     2641 tcggcaaagc ctttaaacgt gtttctcttt ccttgaaagt agtatttgca acttgcggta
     2701 gtttctatgg ttccataact tttttggaat aattcaatca tttcaccatc aatcactttt
     2761 tgacttttcg cttctaaatt agggaactgg tcttttcttt tttcaaacac ttatttcacg
     2821 ctctcttctt ctaggtttat tccataattt tctagcaata atctgtatcc cttaataaaa
     2881 tctttcgtag caaactcttt tccgctttca caaaacttgt ttagtcctat ctgttgaccc
     2941 tcaaaatcaa acaatcgcaa tctggtttta tcatctagac taaatccatt ttcatcgaac
     3001 accatctcta aatgagcata gccatcattg ttatctgaaa acttcatcac atcttcaatc
     3061 gtttcttgat aaggttcata gcgcccacca aaccctgttt catcataagc actcactcca
     3121 gattcatagg tgacaaaatt gataactcct gtacttctat caaagattcc ttttgctaat
     3181 tcttctcttg attcatctcg taagtcatat gctttattga ttaaatatat tccttgattc
     3241 aaaacgtcca tcaacatgaa tcgaccatat ccccactgat aatgaattga cacgcttact
     3301 ttcgttttgc tgttaccata tttgactgta attaacgctt gtgttctttc gcccatcttg
     3361 ttttcctctt ttctctaatt tttataaaga accattttct tgaaatgagt aatagcttgt
     3421 gtttttactt gttacgatga tatggtctaa caattcaata ccaattgttt ttcctgcgtt
     3481 tttcaaattc gtagtaaaac ttttgtcatt ttcacttgga aattcatatc cgctcggatg
     3541 attatgtgat actgcgattt ttgctgaatt gtttagcaaa gctagttgaa aaatctctct
     3601 tggtatcgct actgattgat taattccacc tttgaaaact tcgctataag caatgacctc
     3661 attctttgta ttcagacaca tcactagcaa agattcacta gcattgtttc caattaagtt
     3721 agtagcaaag tttgctagac caaccgagct tgtgactcta taaaaacttc tatcttcact
     3781 gatatatttt tgttcgattt tcaggacatt cttaatcaat ccttcacctt cattctcggt
     3841 cttgttttca acttcaatag attcaagact gctatttccc atatcagaac ccaacaaagc
     3901 atttctgaaa tccaatacct tttcaaagtt atcagtcata accatttcat aggtgctatt
     3961 ccattcattc aaatatttct ctttgatgag aatgaacgct ctcggtttac ttctgccaat
     4021 tctgatattt tgtgtatcgt gaaaaatata attatcaagt aaagtaccat cgtacttttc
     4081 atagttccca ctgatatttg ttaacttgtt taccatttct tgattaactt ctaaaattct
     4141 atctctcatg ttgaacgctt ctttctgttt ttgatttttg gtttttcaaa attctttttt
     4201 gtttggattg ttttagaggg aggtggtttt ttcttcatcg ctatttggtt ttgcatttta
     4261 tgtttcttcc tttcgttcat ttatttatct ttttcgcctg tcttgccttg tcgggagtga
     4321 ttagaatgcg ttcttatctg ccggattaat acaagtgttt aagtaaatta ttttcgtaac
     4381 gcttgcgttt agaaaaaaat ttacgccact tgtattaatc caaagatgag ataagctcta
     4441 aacacttctg acaaggtatg acaggcgaaa aagataatgg gcgtaaggaa ttataaatgg
     4501 caaaaccaaa taaagagcga tgaatttcaa tgctttcagg cgtagcttga aagcctaaaa
     4561 gtcttgaaga atgaaagact ttttaccttc aaagcagacc tagtaatctt aaataggcga
     4621 tggctattta agattacggt gttgacccac taaataaaca gaaatggaac cgatcaagca
     4681 aaatcggact aggcgatggc taggacggtt tgcggtggct ttaggaaacg ttcatggtga
     4741 gatgagcata tctagcaatg tcgggctaag cta
//
LOCUS       DACGLE010000124         4675 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0534
            SAMEA3304449-rid9560453.denovo.131, whole genome shotgun sequence.
ACCESSION   DACGLE010000124 DACGLE010000000
VERSION     DACGLE010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304449
            Sequence Read Archive: ERR985064
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4675)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4675)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702225.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702225.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 20:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,045
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 27 of 67
            Pseudo Genes (incomplete)         :: 25 of 67
            Pseudo Genes (internal stop)      :: 19 of 67
            Pseudo Genes (multiple problems)  :: 4 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4675
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304449-rid9560453.denovo.131"
                     /strain="VREN0534"
                     /db_xref="taxon:1352"
     gene            4..2376
                     /locus_tag="IXT20_002684"
     CDS             4..2376
                     /locus_tag="IXT20_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4861088.1"
                     /translation="MAEEKEPLDNSRLGKSKRKLVRLQNELNEQIEKMFEHQKMTNGQ
                     PMNDKRNGRSWFNQQERIENKVQSLREEIKQQEKQVEKLERQEELKEMGYNKYGGLDM
                     TIENIPRIREEIERFEKGESSFSAATIRKYQRKLETLEQLKEKSEKGKENILPEVQAI
                     IDSGRVTQWKKNPTIYFLKGYRKVALELNENGEFEESKKYQATSEEEKAAVRELLEEA
                     APTDSLKEKENDHFYRVEFNENDPEKGLKSYGGEIVTLELIDQLKTLDNQYAAEAGYY
                     KFYFEEVKNGEVVDQIRMDLGDGVNSNQPIYLELAKLCEQVPEKEQKQTVKNEPVSKE
                     QMQSKETKTKQKGYQKRTAEEIKQEVKSLSQQALDAVKKHTHSPEDVKELLNFMSRFP
                     ERSFRNQLLIEEQFPGATACLGRAALKKEGIYIKKGEQGNKIFVRKTVKGFYEKGRGF
                     VRETEATPEDKKRIESGQIEVIKKPYYTIEKVFDITQTQLKPEDYPKIFPNRVFDFQL
                     DQTGQSELQAGIQAVANNIGIQIRDITESEIYQRELGQARGAYVRNEFTGEEEIVMNT
                     RNTPTQHLATSIHELAHARMHKLSELDTATKELQAEMTSYIVCKHFGMDTSEKAIPYI
                     AAWTKNGQTLDDKEAAERGKIMNDVSRVANEFIQTISNEINQYREREPEIQPAEVQQE
                     HKNTVETAEQKNIVPVGSLWIDKKDGKVMEEDTGRTLHLKDAAFIEDPQHNQVILKGK
                     SYGKEIQTALPSEEFALGNPFRFSLETVSEVAKRKNEIYYEQQLSQKERV"
     gene            2437..3897
                     /locus_tag="IXT20_002685"
     CDS             2437..3897
                     /locus_tag="IXT20_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HAQ4861089.1"
                     /translation="MTEKKMSLIDRCKQMDIVDFARNNGMAVVNKGRDYRLEDHDSFV
                     FDRRKQRFYWNSQNISGDIIELAKLFFIDKEIQDPKQQFKAATDFILKNEAKTERVEN
                     LHFETEKYKDHPVDYQPLTEKGRNYLKEERKLPEWLIDYAEKEGLITELKPKHERQNF
                     LVRDNRLDHAVAFLWKDPQTKETVGASYQGTFIDYERFGERGTYKHIDKNSTANHGFN
                     LKIGDPKQLKFFESSIDLLSYAALNRDQLNDTWLVSMEGLKHHVISHYFGEAVSELRK
                     KQAFPQSIEICVDNDRAGHIFYEKEQLMGAVDPFTNQKVHCERGIANDWQVPKEYKII
                     YEEVAKEEKVTPEAIMAIHKTENNLQLTNQLVSAHKVNASFDQQLSVNDSIEAINLKD
                     ICREVAKELKGCERVDGTYDFDRFYQEKGDINAQILFSYKAEQYYKGYKNHEHEFVPE
                     VKKDWNDQLKHEIQQQEIRKQKRAILFQQGRQQERE"
     gene            3908..>4675
                     /locus_tag="IXT20_002686"
     CDS             3908..>4675
                     /locus_tag="IXT20_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HAQ4861090.1"
                     /translation="MKTVILAEKPSQAKAYADSFSKTTRKDGYFEIQDRLFSGETVIT
                     YGFGHLVELDSPDMYDENWKQWSLEHLPIFPTHYRYHVPKDKKKQFNVVKQQLQSADT
                     IIIATDSDREGELIAWTIIQQAGADQGKTYKRLWINSLEKEAIYQGFQQLRDAGETYP
                     KFEEAQARQIADWLIGMNGSPLYSLLLQQKGISGSFSLGRVQTPTLYMIYQLQEKIRN
                     FKKEPYFEGKAQVTAQNGTFDAKFDPNETQVTQEAFEA"
BASE COUNT         1785 a          776 c         1010 g         1104 t
ORIGIN      
        1 gacatggcag aagaaaaaga accactagac aatagtcgtt tgggaaaatc caaaagaaaa
       61 ttagtacgat tgcaaaatga attaaatgaa caaatcgaaa agatgtttga acaccaaaaa
      121 atgacgaacg gtcaaccaat gaacgacaag aggaatggcc gttcatggtt taaccagcaa
      181 gaacgaatcg aaaataaagt ccaaagtctg agagaagaga tcaagcaaca agaaaaacag
      241 gtagaaaaac ttgaaagaca agaagagcta aaggaaatgg gttataacaa gtacggcgga
      301 ctagatatga cgatcgaaaa tattccaaga atcagagaag aaatcgaacg ctttgaaaaa
      361 ggtgaatcgt ctttttcagc cgccacgatc cgaaaatatc aacggaagtt ggaaacatta
      421 gaacagctga aagaaaaatc agaaaaagga aaagaaaata tcctccctga agtacaagcg
      481 atcattgaca gcggacgagt aacgcaatgg aaaaaaaatc cgaccattta ttttctgaaa
      541 ggctatcgaa aagtcgcttt agaactgaat gaaaacgggg aatttgagga atctaaaaaa
      601 taccaagcca catcggaaga ggaaaaagca gctgttcgtg aattacttga agaggctgcg
      661 ccaactgatt ctctgaagga gaaagaaaac gatcatttct atcgagtaga attcaacgaa
      721 aatgatccag aaaaaggatt gaaaagttat ggtggagaga tcgtaacatt agaattgatc
      781 gatcaactca aaacgttgga caaccaatac gctgcagaag caggttatta taaattctat
      841 tttgaagaag tgaagaacgg agaagtagtc gatcagatcc gaatggatct aggggatggc
      901 gtcaacagta atcaacccat ctatttagag ttagcaaaat tgtgcgaaca agtacctgag
      961 aaggaacaaa aacaaacggt caaaaatgaa cctgtttcga aagaacaaat gcagtcgaaa
     1021 gaaacaaaaa caaaacaaaa gggatatcaa aaacgaacgg cagaagagat taaacaagaa
     1081 gttaaatcac tttcgcaaca ggcactggat gcggtgaaaa agcataccca ttctcctgaa
     1141 gacgtcaaag aacttttgaa ctttatgtct cgttttccag aacgatcctt tagaaatcaa
     1201 ttattgattg aggaacaatt tccaggtgca acggcttgtt taggtcgtgc cgcgttgaaa
     1261 aaagaaggaa tctatatcaa aaaaggggaa caagggaaca agatctttgt cagaaaaacg
     1321 gtcaaaggat tttatgagaa aggacgtggc tttgttcgag aaacagaagc tacaccagaa
     1381 gataaaaaac gaatcgaaag tggtcaaatc gaagtgatca aaaaacctta ctacacgatt
     1441 gaaaaagtgt ttgatatcac acaaactcag ttaaaacctg aagactatcc gaaaatcttc
     1501 ccgaaccgtg tctttgattt tcaattggat caaacagggc agtcagaact tcaagctggg
     1561 attcaagcgg tagccaacaa tatcgggatt caaattcgag atattacgga gagtgaaatc
     1621 tatcaaagag aattaggaca agcacgtggg gcgtatgttc gtaacgaatt tactggcgaa
     1681 gaagaaatcg tcatgaatac tcgaaatact cctacgcagc atttagctac aagtattcat
     1741 gaattagccc atgcaagaat gcacaaactt tcagaattag ataccgcaac taaagaattg
     1801 caggcagaaa tgacttctta tattgtgtgc aaacattttg gaatggacac gtcggagaaa
     1861 gctattcctt atattgcagc atggacgaag aatggtcaga cgttggatga taaggaagct
     1921 gcggaacgtg ggaaaatcat gaatgatgtc agccgtgtcg caaatgaatt cattcaaacc
     1981 atttctaatg aaatcaatca atacagagaa agagaaccag agatccaacc agcagaagtg
     2041 cagcaggagc ataaaaatac tgtagaaacg gcagaacaaa agaatattgt gcctgttggt
     2101 tcactttgga tcgacaagaa agacgggaaa gtaatggaag aagatacggg tcgaacgtta
     2161 catttgaagg atgcggcgtt tattgaagat ccgcagcata atcaagtaat tttaaaagga
     2221 aaaagttatg gaaaagagat ccaaacggcg ttaccttcag aagaatttgc gctaggaaat
     2281 ccattccgat tttcacttga gacagtgtca gaagtggcca aacgaaaaaa tgaaatctat
     2341 tatgaacaac aattgtcaca aaaagaacgc gtataaaaca agtaagaaag actatctgaa
     2401 aaatggatag tcttttttct tttgggaggt ttgaaaatga cagagaaaaa aatgagtctc
     2461 attgatcgat gcaagcaaat ggatattgtg gattttgcac gaaacaatgg aatggctgta
     2521 gtcaataaag gacgtgacta tcgcttagaa gaccatgatt cgtttgtttt cgatcgaaga
     2581 aaacaacgtt tttattggaa tagtcaaaat attagtggcg acattattga attagctaaa
     2641 ctttttttta tagacaaaga gatccaagac cctaagcaac aatttaaagc agccactgat
     2701 tttattctaa agaatgaagc caaaacagaa cgagtagaaa atcttcattt tgagacagaa
     2761 aagtacaaag atcaccccgt tgattatcaa ccacttacag aaaaaggaag gaattatctg
     2821 aaggaagaac gaaaattgcc agagtggctg attgattatg cagaaaaaga aggattgatt
     2881 actgaactaa agccaaaaca tgaacggcaa aattttttag ttcgagacaa tcgtttagat
     2941 catgcagttg cctttttatg gaaagatcca cagacaaaag agactgtggg ggcgagttat
     3001 caaggaacgt ttattgatta tgaacgtttt ggtgagagag gaacatataa gcatatagat
     3061 aaaaattcga cagcgaatca tggctttaac ctaaagatcg gtgaccctaa gcagttaaaa
     3121 ttttttgaga gtagtattga tttacttagc tacgctgcac tgaatcgtga tcagttaaac
     3181 gatacgtggt tagtctctat ggaagggtta aaacaccatg tgatcagtca ttattttggt
     3241 gaggcagtat ctgaattgag aaagaaacaa gcctttcctc aatcgattga gatttgtgtg
     3301 gataatgatc gtgcaggaca tattttctac gaaaaggaac aattaatggg cgcagtcgat
     3361 ccatttacga atcagaaagt acattgtgaa cgaggaatcg ctaatgattg gcaagtgcca
     3421 aaagaataca aaatcattta tgaagaagtc gcaaaagaag aaaaggtcac accagaagct
     3481 attatggcca tacacaagac agaaaacaat ctgcaattaa cgaaccaact ggtttccgct
     3541 cacaaagtca atgcgtcttt tgaccagcag ctttctgtta acgattcaat agaagctatt
     3601 aatttgaaag atatttgtag agaagtcgca aaagaattaa aaggttgtga acgcgtggat
     3661 ggtacgtacg attttgatcg tttttatcaa gaaaaagggg atattaatgc tcagattctt
     3721 ttctcttata aagcagaaca atactacaaa ggctataaaa atcatgaaca tgaatttgta
     3781 cctgaagtga aaaaggattg gaacgatcaa ttgaagcatg agatccagca acaagaaatc
     3841 agaaagcaaa aacgtgccat attgtttcaa caaggcagac aacaagaaag ggagtgaaat
     3901 cgtcatgatg aaaaccgtta ttttagcaga aaaaccgtca caagcgaagg cttatgccga
     3961 tagcttttct aaaaccaccc gaaaagacgg gtattttgag atccaagatc gattgttttc
     4021 gggagaaact gtgatcacgt atggttttgg tcatttagtt gagttagatt cgccagatat
     4081 gtacgatgaa aattggaaac aatggtcatt agagcatttg cctattttcc ctacacatta
     4141 tcgctatcat gttcctaaag ataagaaaaa acaatttaac gtagtgaaac agcaacttca
     4201 atcggccgac accattatta ttgcgacaga tagcgatcgt gaaggggaac tgatagcttg
     4261 gacgatcatt caacaagcag gtgcagacca gggaaaaacc tataaaagac tttggatcaa
     4321 ctccttagaa aaagaagcga tttatcaggg attccagcag ttacgagacg ctggagagac
     4381 ctatccaaaa tttgaggaag ctcaagcacg tcagatcgct gattggttga tcggcatgaa
     4441 cggtagtcca ctatatagtt tactcttgca gcaaaaaggc atatcaggaa gcttctcctt
     4501 aggcagagtt cagactccaa cgctttatat gatttatcag ttgcaagaaa agattaggaa
     4561 cttcaagaaa gaaccctact ttgaaggaaa agcccaagta accgctcaaa atggcacatt
     4621 tgatgccaag tttgatccca atgaaaccca agtgacgcaa gaagcatttg aagcg
//
LOCUS       DACGLE010000125         4661 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0534
            SAMEA3304449-rid9560453.denovo.132, whole genome shotgun sequence.
ACCESSION   DACGLE010000125 DACGLE010000000
VERSION     DACGLE010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304449
            Sequence Read Archive: ERR985064
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4661)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4661)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702225.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702225.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 20:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,045
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 27 of 67
            Pseudo Genes (incomplete)         :: 25 of 67
            Pseudo Genes (internal stop)      :: 19 of 67
            Pseudo Genes (multiple problems)  :: 4 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4661
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304449-rid9560453.denovo.132"
                     /strain="VREN0534"
                     /db_xref="taxon:1352"
     gene            complement(173..475)
                     /locus_tag="IXT20_002687"
     CDS             complement(173..475)
                     /locus_tag="IXT20_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4861091.1"
                     /translation="MLMEGKTQLWFKFDPSNRFVKDFYKVWDSEVFFLAIEDSLLINL
                     YYSNKNYFKIPATKTRMKKDVYFLFDIVTDVPDARSDHRRYDYLKYTFVDPERYKD"
     gene            complement(695..1061)
                     /gene="ssrA"
                     /locus_tag="IXT20_002688"
     tmRNA           complement(695..1061)
                     /gene="ssrA"
                     /locus_tag="IXT20_002688"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(1232..2005)
                     /locus_tag="IXT20_002689"
     CDS             complement(1232..2005)
                     /locus_tag="IXT20_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAQ4861092.1"
                     /translation="MSNYRAITFFDLDGTLLDAKSKITPEVAKAMNALKENNVLPVIA
                     TGRTEAEIHSIMEDAGITSAVTMNGSFIRVDGEEIYSQTFSSEECQKMYDHVQLNGHE
                     LSYYNDQKIWCTGHSQTMINAYDFIHSDVPEIDPLGFKENTVNMLLILSQSGDEYYHE
                     HFPELTFYRNGPYSIDTVKKGISKGAGVKRLQKELQLEKVPTFGFGDGPNDFALLEAC
                     DNKIAMGNAYDGLKELSTFITKKNTEGGIVHALKYFDLI"
     gene            2149..2499
                     /locus_tag="IXT20_002690"
     CDS             2149..2499
                     /locus_tag="IXT20_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HAQ4861093.1"
                     /translation="MIFFSIGILFLIIGSLFRILPSKGNLPFYGYHSPLAAKTDAHWR
                     LAQKTSGNWFFLMGLLMALIGYYLKTSGHTNYFLIEMLLLVFPIMPIFIMTEKKLQKY
                     DLETGGNDNEYFND"
     gene            2480..3196
                     /locus_tag="IXT20_002691"
     CDS             2480..3196
                     /locus_tag="IXT20_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ4861094.1"
                     /translation="MNILMIEDNQSVSEMMQMFFLNEGWEATFKYDGKEGLDAFLENP
                     QHWDMITLDLNLPTLDGMLVGREIRKVSSTVPIIMLTARDSESDQVIGLEMGADDYVT
                     KPFSPLTLIARMKALHRRSELAEAREEAVVSDDHFDIETSHFKMNTKTREAYLDNRPI
                     EGLTPKEFDLLFTLAKKPRQVFSREQLLELVWDYQYFGDERTVDAHIKKLRQKIEKVG
                     PQVIQTVWGVGYKFDDSGVA"
     gene            3196..4644
                     /locus_tag="IXT20_002692"
     CDS             3196..4644
                     /locus_tag="IXT20_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAQ4861095.1"
                     /translation="MRYLYQQLLAFCGMVALILLIVGVSFTQLTKQTIEENNYQQLFG
                     YAESVEKTTQTYADTLPQFSQDQAFQNALLLTEQVLTEQNVNFIFIDKNERVIYPTNT
                     ALLNFTLTADQWKNLRNGRQEKFTSNKNILGQAQATSYALVPFNLNHEFYGALVVSQP
                     ARNIDNSVRPITLNLFKGFIFSSIVAVIASYAFAAFQVKRINRLRNATKEVTNGNFDV
                     QLPVHDKDEFDELADDFNKMTNSLKESQAEIEEQENRRRQFMADASHEMRTPLTTING
                     LLEGLEYNAIPENQRENAIKLMKNETERLIRLVNENLDYEKIRTNQISMVIKKFNGTE
                     TLKNIVAQLEAKAEAAGDTLTLKAVDDIDVYADYDRFVQIMVNIIQNAIQFTENGQIT
                     ITLEKGYLETIITIEDTGIGMSEQQMKSIWDRYYKVDPSRKNTKYGESGLGLPIVQQL
                     VRLHKGKLEVESELGKGTKFAVRLPDVEIKKE"
BASE COUNT         1549 a          823 c          899 g         1390 t
ORIGIN      
        1 cttataattg gagtggtagt taattagtga cttatttttg attttatagc actgatacta
       61 taaaatatag atatcatcat attacacaat cttaatacta acttaaaaaa tatccctttt
      121 ataagtatgg tgataaaatc cgttccgggc tacctttttt aggtagccta ctttaatctt
      181 tatacctttc tggatcaacg aaagtatact tcaaatagtc ataacgccga tgatcgctcc
      241 gtgcgtctgg cacatcagtc acgatatcaa acaaaaaata cacatctttc ttcattctgg
      301 tttttgttgc cggaatttta aagtagttct tattagagta atagagattg attaataagc
      361 tatcttcgat tgctaaaaag aaaacttctg aatcccacac cttataaaaa tctttgacaa
      421 atctattcga aggatcaaat ttaaaccata attgtgtttt tccttccatc aacataatgt
      481 tcacctcaaa aagagtatac gaactaatgt tctttttgta aagtaggaat atcattgata
      541 atatggatgg aacatatata ttaaaaagtg ttctttttga atgctatttt gaatgccatt
      601 ttcattcatt taacgaaaat ctgcgaaaat aaaaaatgct caaaccctta taaaataagg
      661 atttaagcat ctagcgaaat ctgctgaaat ctcacaatgg agacggcggg agtcgaaccc
      721 gcgtccaaac acatcgccac ttaaaaatct acgttcatag tcaactcatt taaggattca
      781 ctttacagct tgccgagtga caggcattct gtatcgctag tctgatgttc tctttttcaa
      841 cttacagacg gaaagttgaa acgtatccca ctaaagttag gacccttact cgagcacatg
      901 ggcgatgccg agaggatcta cgctaactgt ttttaggcag ctaaagcgta agagttgttt
      961 tcgtttttag cagttatatt taactgtaac gtttttacgt agacgtaacc tacgaaacgc
     1021 aattcaagct cgactgtgcc tgtcgaatcc gtaacgtccc cgttagctaa caagggttac
     1081 gtgtattttt catacaaaat tagtatagca tatattggga taaattcaat aaaaaagcga
     1141 atcatatccc attatttata taataactgc ccctttctta agaaaaaata agcgattttc
     1201 ttaagaaaag aggtagcttc aaaaaaagat cttaaatcag atcaaaatac ttcaatgcgt
     1261 ggacaattcc gccttctgta tttttctttg tgataaaggt agaaagttct tttaatccgt
     1321 cataggcgtt gcccatagcg attttgttgt cgcaagcttc caacaaggca aaatcattcg
     1381 gaccatcgcc aaatccaaaa gtaggaactt tttccagctg aagttctttt tgcagacgtt
     1441 tgacacctgc cccttttgag attcctttct tcactgtatc gatcgagtaa ggaccattcc
     1501 gataaaaagt tagctcagga aaatgttcgt gatagtattc gtcacctgat tgagataaga
     1561 tcagcaacat atttactgtg ttttctttaa agcctaaagg atcaatttcc ggaacatctg
     1621 aatgaatgaa gtcgtaagca ttgatcattg tttggctatg accggtacac cagattttct
     1681 gatcgttata gtaagaaagt tcatgaccgt tcaactgtac atgatcatac attttttgac
     1741 attcttctga agaaaatgtt tgagaataga tttcttcgcc gtctactcga atgaaagaac
     1801 cattcatggt gaccgcagaa gtaatgcccg catcttccat gatagagtgg atttctgctt
     1861 ctgtacgtcc tgtagcgatg acggggagaa cattgttttc ttttaatgcg ttcatagctt
     1921 ttgccacttc gggtgtgatt tttgatttcg catctagtaa tgttccatca agatcaaaga
     1981 aagtgattgc acgataattt gacacaaata aaactccttt tttattggaa taagtttatc
     2041 ttaacaaaga atgaacaaaa gacaaacctt tcgtgaagaa ttcctgaaca tattggttca
     2101 gtcaacaatt tagagtatac tataagaaga gaatttagga gggaaagaat gatttttttc
     2161 agtattggta tcttattctt aataattggc agtctttttc ggattctgcc ctcaaaagga
     2221 aatctgcctt tttatgggta tcattcgcct cttgccgcaa aaacagacgc tcattggcgg
     2281 ctggctcaaa agacaagcgg aaactggttt tttcttatgg gcttactgat ggcattgatc
     2341 ggctattatt taaagacatc tgggcatacg aattattttc tgattgaaat gcttctgcta
     2401 gtatttccaa ttatgcctat ttttattatg acagaaaaga aattacagaa atatgacctg
     2461 gaaactggag ggaacgataa tgaatatttt aatgattgaa gataatcaat ctgtttctga
     2521 aatgatgcag atgtttttct taaatgaagg ttgggaagca actttcaagt atgatggaaa
     2581 agaagggctc gatgcctttt tagaaaaccc acaacattgg gatatgatta cactagacct
     2641 gaatttgccc acactggacg gtatgctagt aggaagagag atccgcaaag tttcaagtac
     2701 tgtgccgatt atcatgctga cagctagaga ttctgaaagc gatcaggtga tcggcttaga
     2761 gatgggggca gatgattatg tcacaaaacc tttcagtcct ctgacgctta ttgctcgaat
     2821 gaaagctttg catcgccgta gtgagttggc agaagcaaga gaagaagcag tagtttcaga
     2881 tgatcatttt gatatcgaga ctagtcattt caagatgaat acgaaaacac gagaagcgta
     2941 tctggacaac cggccaattg aaggactgac gcctaaagag ttcgatctat tgtttacttt
     3001 agcaaaaaaa ccacgtcaag tattctctcg ggagcagttg cttgaattag tctgggatta
     3061 tcagtacttt ggtgatgaac ggacagtgga cgcccatatc aagaaattac gacaaaaaat
     3121 cgaaaaggtc ggaccacaag tcatccaaac tgtttgggga gtcggctaca aattcgatga
     3181 ttctggtgtt gcctaatgcg ttatttgtat cagcaactct tagctttctg cggaatggtc
     3241 gcgttgatct tattgatcgt tggagtttct tttacacaac tgacaaaaca aacgattgaa
     3301 gaaaacaatt accagcaact gtttggatat gctgaatcag ttgaaaagac aacgcaaacc
     3361 tatgccgata cgctcccgca gtttagtcaa gatcaggcgt ttcaaaatgc attgctgctg
     3421 acagagcaag tactgacaga acaaaatgtg aactttatat ttatcgataa aaacgaacgt
     3481 gtcatttatc cgactaatac agctttactg aattttacct taacagctga tcaatggaaa
     3541 aatctgagga atggacgtca agaaaaattc acatcgaata aaaatatttt aggacaagca
     3601 caggcgacct cttacgcact tgtgccattt aatttgaatc atgaattcta tggagcactt
     3661 gtagttagtc aacctgcgcg aaatatcgat aacagcgttc gtccaattac actgaacctg
     3721 ttcaaaggat ttattttttc cagtatcgtt gcagttattg ccagctatgc ttttgcagca
     3781 ttccaagtta agcgtatcaa tcggttaagg aatgcgacga aagaagtgac gaatggtaat
     3841 tttgatgtac agctccccgt acatgacaaa gatgaatttg atgagttagc agatgatttc
     3901 aataaaatga cgaattcatt gaaagaatct caagcagaaa ttgaagaaca agaaaatcgt
     3961 cgaagacaat ttatggcaga tgcttctcac gagatgagaa cacctcttac gacaatcaac
     4021 ggacttctgg aagggttaga atacaatgca atccctgaaa atcagcgaga aaacgcgatt
     4081 aaattgatga aaaatgaaac agagcgtctg atccgtttgg tgaatgaaaa cttggattat
     4141 gaaaaaatcc gtacgaatca aatcagtatg gtcattaaaa aattcaatgg aacagaaaca
     4201 ttgaaaaata ttgttgcaca gttagaagct aaagcagaag cagcaggaga tacattgaca
     4261 ttgaaagccg ttgatgatat tgatgtttac gctgattatg accgatttgt ccaaatcatg
     4321 gtcaatatta ttcaaaacgc gatacagttt actgaaaacg gccaaatcac gattacttta
     4381 gaaaaaggct atcttgaaac gatcatcacg attgaagata cgggaattgg aatgtctgaa
     4441 caacaaatga aaagtatctg ggatcgttat tataaagtag atccttcaag aaaaaataca
     4501 aaatatggtg aatcaggatt aggtttacca atcgtgcagc aacttgtacg cttgcataaa
     4561 ggaaaattag aagtagaaag cgaactagga aaaggaacaa aattcgctgt tcgtttgcct
     4621 gacgtagaaa tcaaaaaaga gtaaaaaaca agcggtataa a
//
LOCUS       DACGLE010000126         4435 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0534
            SAMEA3304449-rid9560453.denovo.133, whole genome shotgun sequence.
ACCESSION   DACGLE010000126 DACGLE010000000
VERSION     DACGLE010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304449
            Sequence Read Archive: ERR985064
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4435)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4435)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702225.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702225.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 20:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,045
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 27 of 67
            Pseudo Genes (incomplete)         :: 25 of 67
            Pseudo Genes (internal stop)      :: 19 of 67
            Pseudo Genes (multiple problems)  :: 4 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4435
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304449-rid9560453.denovo.133"
                     /strain="VREN0534"
                     /db_xref="taxon:1352"
     gene            <1..2251
                     /locus_tag="IXT20_002693"
     CDS             <1..2251
                     /locus_tag="IXT20_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1906 domain-containing protein"
                     /protein_id="HAQ4861096.1"
                     /translation="TIYGLIEGLQVELGITNLVANFGPTTEKMYDNQVTPKWGKNLPK
                     NIVFLIQGAFWCKGINPGGFDGVYTPYLDTAVKELQTDAGFNGSAVTGVLDAKWAKAL
                     FDMSAFVLVPGGDSKIRKMQQWLNVNYLEYTGIMPCDGIYQRNSNQALIFALQAELGY
                     SPSEATGSYGNGTTSKTYPVSEGNSGNYVRIIQYGLYVNGYFEDGTFDGIFTKYMGLE
                     VLAFRKFMVLPEYTEIADVVVIKGLLSSAGDIRRSADGADTSTQLTRSQIQTLVDNDI
                     KIVGRYLTGTVGIGKDERNKYLTTEELNNIFSKNLSVFPIYQDGGAALAYFTYDRGLS
                     DGKKAIDAAKNLNIPLTTVIYFAVDLDMLGEEILAYAGEYFKGVSAVMSYSGYQTGVY
                     GTRNVCSQIINNGYASFAFVSDMSTGYSGNLGFPMPRQWSFDQFVEFTIGSGNGAVGI
                     DLIATSGRDSGFNELSNDTNNDNYIAKYNQKVINQMVRAYQYLSQAGLDNPWNPHLTF
                     YRYVNYSGLSWDIISSPVTEHDRKIYDEYRNKLTNSEGLYNYFIDPNTGSIIGLPHLI
                     VTLQSQMFLADTINDSVSDFAGWLGDLMTCWGEVKKLGIQMEQGVFALVGTAAGGTFS
                     LEDLYQDVDAVNVYTLAKRNSDSTALSALKTYYCDKGAIDRFNQFVKNRFGSSDKIAS
                     VTNSLLENDETVDILGVGIISFFIDKIVNNSLGTDFTYLDFIGDEGKVANGFSEKIIQ
                     FQENRSKLN"
     gene            2331..2726
                     /locus_tag="IXT20_002694"
     CDS             2331..2726
                     /locus_tag="IXT20_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4861097.1"
                     /translation="MMIMEEIEWNNLTQNNKNNSGCGIIFMFVIVLGIVVLLITVKIV
                     DYFANTPEEQIQRKLPEFERVPPYDPRDNSFQVKKLKESPNEMWFEVTVESSDFKEKK
                     KEYNYIVKAKVSKRFIFNPKLEIYSVVIE"
     gene            3051..3668
                     /locus_tag="IXT20_002695"
     CDS             3051..3668
                     /locus_tag="IXT20_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAQ4861098.1"
                     /translation="MRKFFYIRVSSYDQSIERQLISAKELGIPEEYIFVEKASGKDFK
                     RPEYQLMKRMFRKGDLLYLQSLDRLGRNKQMILEEWRELTKEKQIDISVLDMPLLDTA
                     SYKQLDGLETLISDLILQLLSYMAEDERKRINSRQREGVEAAKQKGVKFGRKKIALPP
                     EFPAIYSDWKAGKITAVYAMDQLGMKRNTFYRRVKEYEHTLHHFS"
     gene            3709..4008
                     /locus_tag="IXT20_002696"
     CDS             3709..4008
                     /locus_tag="IXT20_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000366408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4861099.1"
                     /translation="MDLTNQRVVFTGTLQQMTRTQAIGLVHSLNGHCQSSVTSKTNFL
                     VCGQYSKSLFDDSLYTKKEQTARDLIALGHEITILSEVEFYALISQQLKEWQRYL"
     gene            4042..4209
                     /locus_tag="IXT20_002697"
     CDS             4042..4209
                     /locus_tag="IXT20_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000824191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAQ4861100.1"
                     /translation="MKRKSYDRWGIEITSFNRNIAFHVECDCGELAKSVYQKNYFYCP
                     KCHRRYGVHGT"
     gene            complement(4254..>4435)
                     /locus_tag="IXT20_002698"
     CDS             complement(4254..>4435)
                     /locus_tag="IXT20_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ4861101.1"
                     /translation="GRYKGRPIEYSINSTDPQKRLVFKTVVDMLKQGIPVAEIAKENG
                     ISRPTIYKIKKANDL"
BASE COUNT         1525 a          601 c          895 g         1414 t
ORIGIN      
        1 tacaatctat ggacttattg aaggactaca agtagaactt ggtattacta atctagtagc
       61 taattttgga cctaccactg aaaaaatgta cgataatcag gttactccta aatggggcaa
      121 aaatctacct aaaaatattg tatttcttat tcaaggtgcc ttttggtgta aggggattaa
      181 tccaggtggc ttcgatgggg tatatactcc atatttagat actgctgtaa aagagttgca
      241 gactgatgca gggtttaatg gaagcgctgt aactggagtt ttggatgcga aatgggccaa
      301 agcgttattt gatatgagtg cttttgttct tgttcccggc ggagatagta aaattagaaa
      361 aatgcagcaa tggttaaatg taaattattt agaatacacg ggaataatgc cttgtgatgg
      421 tatttatcaa agaaattcaa atcaagcatt aatttttgca ttacaagctg aactaggata
      481 ttctcccagt gaagcaacag ggtcttatgg taatgggaca actagtaaaa cttatcctgt
      541 gtctgaaggt aactcgggaa actatgtaag aataattcaa tatggattat atgttaatgg
      601 ttattttgaa gacggtacat tcgatggtat tttcactaaa tatatgggtt tagaagtact
      661 ggcattccga aaatttatgg tattgcctga atatactgaa atagcagatg ttgttgtaat
      721 aaaaggacta ttgtctagcg ctggagatat tagaagatct gcagatggcg cagatacttc
      781 tacgcaatta actagatctc aaatccaaac attggttgat aatgatataa aaatagttgg
      841 tagatatctt actggtactg ttggaattgg aaaagatgaa cgaaataagt atctaactac
      901 agaggaattg aataatattt tttctaaaaa tttatctgtg tttcctatct atcaagatgg
      961 aggtgcagca ttagcttatt ttacttatga tcgtggctta tcagatggta agaaggctat
     1021 agatgcagca aaaaatctaa atataccatt aacaacagtt atttattttg ctgttgatct
     1081 agacatgtta ggggaagaaa ttcttgcata tgctggagaa tattttaagg gggtatctgc
     1141 tgtaatgtca tatagcgggt atcaaacggg tgtttacgga acaagaaatg tttgttctca
     1201 aataatcaat aacgggtatg cttcgtttgc atttgtttca gatatgtcga ctggttatag
     1261 tgggaattta ggttttccta tgcctagaca atggtctttt gatcaatttg ttgaatttac
     1321 cataggaagt ggcaacggag ctgttggtat cgatttaatc gctacatcgg gtagagatag
     1381 cggatttaat gagttatcaa atgatactaa taatgataat tacattgcca aatacaatca
     1441 gaaggttata aatcagatgg tacgtgcgta tcaatatcta tcacaagctg gtcttgataa
     1501 tccttggaac cctcatttga cattttatag atatgtaaat tattctggtt tgtcatggga
     1561 catcatttct tctcctgtaa cagagcatga tagaaaaatt tatgatgaat ataggaataa
     1621 attaactaat agcgagggcc tgtataatta ttttatagat cctaatactg gttcaataat
     1681 aggtttgcct cacttaattg tgacgttaca atcacagatg tttctagccg acacaatcaa
     1741 tgatagtgtt tctgactttg caggttggtt aggagattta atgacttgct ggggtgaagt
     1801 taagaaatta ggaatacaaa tggagcaagg tgtatttgca ttagtaggta cggctgcagg
     1861 tggaacattt agtctagaag atttatacca agatgttgac gcggtaaatg tttatacctt
     1921 agcaaaaaga aattctgatt caacagcttt atcggcttta aaaacgtact attgtgataa
     1981 gggtgctatt gatagattta atcaatttgt taagaacaga tttggttctt cagataaaat
     2041 cgcttcagta acaaatagtt tattagaaaa tgacgaaaca gttgatatct taggtgtagg
     2101 gataatttca tttttcattg ataaaatagt taataattca cttgggacag attttactta
     2161 tcttgatttt attggagatg aggggaaagt tgcaaatgga ttttctgaaa aaattattca
     2221 atttcaagaa aatagaagta aacttaatta agaatcgttt actatttaaa cagtgtcctt
     2281 ttccttttta aatatatacg ataaaataag tatatgaatt gtaaaaggac atgatgatta
     2341 tggaagaaat agaatggaat aatctgactc aaaataataa aaataattct ggatgtggaa
     2401 ttatctttat gtttgttata gttttgggaa tagtagtact tttaataact gtcaagatag
     2461 tagattactt tgcgaatact cctgaagaac aaatacagag aaaacttcct gagtttgaaa
     2521 gagtaccacc ttatgatcca agagataatt catttcaagt aaaaaaatta aaagagtctc
     2581 caaatgaaat gtggtttgaa gttacagttg aatcaagcga cttcaaagaa aaaaagaaag
     2641 aatacaatta tattgtaaag gcaaaagtat caaaaagatt tatttttaac cctaaattag
     2701 aaatatatag tgttgtaata gaataatagt gttaaaaaaa ttgtaaaagt taaagaagtc
     2761 aaattctgat gtgaaaaata aaatatacca tggaagacat aaaagaaaaa ctggataact
     2821 agtttattta atggcagacc gttaaggcct gccattttta tgtattttag cggaataaat
     2881 ataatcatgc gggagtgtaa acttactgtt tcaaaaagta tactttttga catgccttga
     2941 ttttctttgt ttatgtatgg cataataacc acataataca atttgaaacg tttctgattt
     3001 aatctgattg ttttgaaaca attttagtga aaaaagggta gataaaaacg atgagaaaat
     3061 ttttttatat ccgggtcagt agttatgatc aatcgatcga acgacaactg atttccgcga
     3121 aggaattagg aatccctgaa gaatatatat ttgtagaaaa agccagcggc aaagatttta
     3181 aacgccctga atatcaatta atgaaacgga tgtttcgaaa aggagattta ctctatctac
     3241 aatcattaga tagactaggc agaaataaac aaatgatttt agaagaatgg cgagaattaa
     3301 ctaaggaaaa gcagattgat atttctgtgt tagacatgcc tctgttagat actgctagtt
     3361 acaagcaact agatgggtta gagacgctta tctctgactt aattctgcaa ttactcagct
     3421 atatggccga agacgagcgg aaacgaatta atagccggca aagagaaggc gtagaagctg
     3481 cgaaacaaaa gggtgtaaaa ttcggcagaa agaaaattgc tttacctcct gaatttcctg
     3541 cgatctattc tgactggaaa gcaggaaaga taactgcagt ctatgctatg gatcaattgg
     3601 gcatgaaacg aaataccttt tatcgacggg tgaaagaata tgaacatacc ttgcaccact
     3661 tttcttgata gatataacag ctattagacg ggaagggaga gtttgaaaat ggacttaacg
     3721 aatcaaaggg tcgtttttac tggaacgctg cagcaaatga cacgaaccca agccatcggt
     3781 ttggttcact ctttaaacgg ccattgtcaa tcaagtgtta cttcaaaaac aaatttcctg
     3841 gtttgtggtc agtattctaa gtcgttgttt gatgattctc tctatacaaa aaaggaacaa
     3901 accgcgcggg acttgattgc gttaggtcat gagattacca ttttatcaga agtcgaattt
     3961 tatgcgttaa ttagtcaaca gttgaaagaa tggcaacggt atttataatc gttagtaaaa
     4021 tttacaagga ggaaaatttt tatgaagcga aaatcttatg atcgttgggg cattgaaata
     4081 actagtttta atcgaaatat tgcttttcac gtggagtgtg actgtgggga attagcaaaa
     4141 agtgtttatc aaaaaaatta cttttactgt cctaaatgcc atcgccgata cggggtacat
     4201 ggaacataga aacgaaatag tgcggtagaa aagcgaatga atgtagtata tttctacaaa
     4261 tcattcgctt tctttatttt ataaatagtt ggtcgagaga tcccgttttc ttttgcaatc
     4321 tctgcaactg gaatgccttg ctttaacatg tccaccacgg ttttaaaaac taagcgtttt
     4381 tgcggatctg ttgagttaat agaatactcg attggtcgtc ccttatatcg gccgg
//
LOCUS       DACGLE010000127         4405 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0534
            SAMEA3304449-rid9560453.denovo.134, whole genome shotgun sequence.
ACCESSION   DACGLE010000127 DACGLE010000000
VERSION     DACGLE010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304449
            Sequence Read Archive: ERR985064
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4405)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4405)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702225.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702225.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 20:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,045
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 27 of 67
            Pseudo Genes (incomplete)         :: 25 of 67
            Pseudo Genes (internal stop)      :: 19 of 67
            Pseudo Genes (multiple problems)  :: 4 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4405
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304449-rid9560453.denovo.134"
                     /strain="VREN0534"
                     /db_xref="taxon:1352"
     gene            complement(<1..2267)
                     /gene="esp"
                     /locus_tag="IXT20_002699"
     CDS             complement(<1..2267)
                     /gene="esp"
                     /locus_tag="IXT20_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="repetitive surface protein Esp"
                     /protein_id="HAQ4861102.1"
                     /translation="MVSKNNKRVFLEKTKKRVLKYSIKKLSVGVASVLVGAGLVLGTT
                     ELVKAQDEISPSTPLETAISSVQVGDKVASGNTFQENPGYTKNYNFSDLQFSPQELTG
                     DTLKGNTIGFEVYGKHNIAASTKNWEIRLQLDERLAKYVEKIQVDPKKGIGSSRRTFV
                     RINDSLGRPTNIWKVNYIRASDGLFAGAETTDTQTAPNGVITFEKSLDEIFKEIGIDN
                     LKTDRLMYRIYLVSHQDDDKIVPGIDSTGYFLTDSDDFYNSLDVSENNPDQFKHGSVS
                     AKYEEPNTQTKDGSGSTGANGAIILDHKLTKNYNFSYSASAKGTPWYANYKIDERLVP
                     YVAGIQMHMVQADKVTYDVSFESGKKVADLAIERRKDHENYGVGSITDNDLTKLIDFA
                     NASPRPVVVRYVLQLTKPLDEILKEMKTAAQVEGNEPFGEDFIFDCWLSDTNKKLIQN
                     TYGTGYYYLQDIDGDGNPDDKEESGDTNPYIGKPELEEVYDVDTTVKGKVFIHELAGT
                     GHKAQLVDKEGNVLAEKDIAPNEKDGAPVSGTVDFEFTGVDSSKLIAKDELKVQIVST
                     GFDKAEEGSTIIKESPKAIDKQTVVVGFKPDAKESIRNNQSLPDNATYSWKTEPDTSK
                     ATDNTKGVVTVTIGNRTFDVAVEFAVEASQAMENNATYDAIETQPETTVQSGKPKFDK
                     DDVRLSNNPFAIDDTFDETQGKVTIDSATGEVTFTPEKGIGEDKPTKVTIPVSVTYED
                     GSVGKTELIVTVGKG"
     gene            complement(2882..3316)
                     /locus_tag="IXT20_002700"
     CDS             complement(2882..3316)
                     /locus_tag="IXT20_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4861103.1"
                     /translation="MRLYHLRSLEELRKLDFNKKFFWEISDLSCFVESERLSNGNNSV
                     LNEYDKVVINKVKSLCEEARESISHINGSNFKQTLFNLIKLDAKISSYLFFLIHDEFM
                     DYQKLDQLIERESWEDYYVGLTFSEKLNNYKLIDYKYLSESN"
     gene            complement(3333..>4405)
                     /locus_tag="IXT20_002701"
     CDS             complement(3333..>4405)
                     /locus_tag="IXT20_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YSIRK-targeted surface antigen transcriptional
                     regulator"
                     /protein_id="HAQ4861104.1"
                     /translation="KKLLTDNIKKTKDFHFFTGDFNEMFLLYMFKTRYYLFGPFRANN
                     IDKVFFKLKMNNLNVAMADRERLYNSLQNLTLYSLGDIRDILILVHYFFTGKIEDLFH
                     EPLIEYTENLSKTIEQIKIDNLLSQNYDPEIYLFLYENKILEYVKNGDIRNLENMVFN
                     LSNGIIPSVSGDTIRSEKNYSIIVFEKLAQTSITLGMDIIEAYQSRDALIQENELAVS
                     LPEVLKVRDSGIVYYTKEIGKTKIEHLSPLISSVVQFIGLNIYKRITVKEIANYFSVS
                     ETKLRESFKNEMHITIYNYISKRKISTAKIMLKSNHTISEVSLGLGFSDSSHFSRVFK
                     KYAGVSPKQYQLGLVDNFNNIS"
BASE COUNT         1378 a          827 c          580 g         1620 t
ORIGIN      
        1 ataccttttc caactgttac gattaattct gttttcccta ctgatccatc ttcataagtt
       61 acagaaacag gaatcgttac tttagtaggc ttatcttctc caataccctt ttcaggcgta
      121 aaggttactt ctccggtcgc tgaatcaata gttactttcc cttgtgtctc atcaaaagtg
      181 tcatcaattg caaaaggatt atttgataaa cgaacatcat ctttatcaaa tttaggtttt
      241 ccactttgaa cagttgtttc tggttgagtt tcaattgcat catatgttgc attattttcc
      301 atagcttgag aagcttctac ggcaaactct acagccacat caaaagttcg atttccaatc
      361 gtaaccgtta caacaccttt agtattatcc gtagctttag aagtatcagg ctctgttttc
      421 catgaataag ttgcattgtc aggtaaactt tgattatttc taattgattc tttagcatct
      481 ggtttaaacc cgactactac agtttgctta tcaatagcct ttggtgattc tttaataatt
      541 gttgaacctt cttctgcttt atcaaaacct gtagaaacga tttggacttt taattcgtcc
      601 ttcgcaatta atttacttga atccacacct gtaaattcaa aatctacagt acccgaaact
      661 ggcgctccat ctttttcatt tggagcaata tctttttctg ctaacacatt accttcttta
      721 tcaacaagtt gagctttgtg acctgtacct gctaactcgt ggatgaacac tttcccctta
      781 accgttgtgt caacatcgta tacttcttct aattcaggct ttccaatata tggattggtg
      841 tccccgctct cttctttatc atcaggattt ccgtcaccat caatatcctg caaataatag
      901 taacctgttc cataagtgtt ctgaattagt tttttattag tatccgataa ccaacaatca
      961 aagatgaaat cttctccaaa cggttcattc ccttcaactt gtgcggctgt tttcatttct
     1021 tttaagattt catctaatgg tttagtcaac tgtaaaacgt aacgaacaac aaccgggcgt
     1081 ggacttgcat tagcaaaatc aataagtttg gtaagatcat tatcagtaat tgaacctaca
     1141 ccataatttt catgatcttt gcgccgttca attgctaaat cagccacttt tttccctgat
     1201 tcaaaagaaa catcatatgt tactttgtca gcctgaacca tgtgcatctg tatacccgct
     1261 acatatggaa ctaatcgttc atcaattttg taattagcat accaaggcgt gccctttgcg
     1321 gaagctgaat atgagaaatt ataatttttc gttaacttat gatctaagat tatagctcca
     1381 ttagcacctg tggatcctga accatctttt gtttgagtat taggttcctc atattttgca
     1441 cttactgatc catgtttaaa ctggtcagga ttattttcag atacatctaa gctattatag
     1501 aaatcatctg aatctgttaa aaagtagcct gtactatcaa tacctggtac aattttatca
     1561 tcgtcttgat gacttaccaa gtagattcga tacattaaac gatcggtctt gagattgtct
     1621 atgcctatct ctttaaaaat ttcatctaag ctcttttcaa acgtaattac cccattagga
     1681 gcagtttgtg tatcagttgt ttcagcccca gcaaataatc catcacttgc acgaatataa
     1741 ttgaccttcc aaatatttgt aggtctaccc agcgaatcat taattcttac aaaggtccgt
     1801 ctagaagatc caattccttt ctttggatcg acctgaattt tttctacata tttagctaat
     1861 cgttcatcta attgaagacg gatttcccag tttttagttg atgctgcaat attatgtttt
     1921 ccgtaaactt caaatccaat tgtattgcct tttagtgtgt ctccagttag ttcttgagga
     1981 ctaaattgta aatccgaaaa gttataattt ttcgtataac caggattctc ctgaaaagta
     2041 ttccccgaag ctactttgtc acctacttgt acggagctta tcgctgtttc aagtggggtg
     2101 cttggagaaa tttcatcttg tgctttaact agttcagttg ttcccaagac aagaccagca
     2161 ccaaccaaaa cagatgcaac cccaacactt aattttttga tactgtactt caatactcgt
     2221 ttctttgttt tttctagaaa tactctctta ttattcttgc taaccattat tcaaatcaac
     2281 tcccttaata gttattttcc tagtaagaat aaaagataaa tctgatacgt attattcaca
     2341 gaataggaat cttactaaac ttatgttaac taatttactt aaaaaatttc atgcacatta
     2401 caacaaaaaa agtacatttc ttcatgtttt tcggatattt gtgcttttta accattcagt
     2461 agctgaaaac atataaaaag atatgtatgt attctttctg aaattaagtt catttctatg
     2521 cgatgaagca tgttttcaaa atattactct ttcttagatt aaacttcaga gtattcaaat
     2581 agcggaataa ggagccttat cccgcattac tgaatgagac tgtgacataa gtaaaaaggt
     2641 gacttcaaat tccttcttcg gaatttgaaa tcaccttctt tataattata aagtatcggt
     2701 ttcacagcat ttctgaatga ttcctagaat cttaaacgaa tgattggagt tatcaacatt
     2761 ttttcactcg tttttagcga aacaaaaaat gacatttgtc acagcctcat taaattattg
     2821 attctttttc tttataattg aattgtccat atttaaagtg tctagtactc tacatgaata
     2881 attaattgct ttcactcaaa tacttataat ctattaactt gtagttgttt aacttttctg
     2941 aaaaagttag acccacgtaa taatcttccc agctttctct ttcaattaac tgatccaatt
     3001 tttgataatc cataaattca tcatgtatta aaaagaataa ataacttgat attttagcat
     3061 ctagcttaat cagattaaat aatgtttgct taaaattgct accattaata tgactgatag
     3121 attctctagc ttcttcacat aaacttttta ctttattaat tactactttg tcatattcat
     3181 ttaacacact attattacca ttacttagac gctcagattc tacgaaacaa ctcaaatcag
     3241 aaatttccca aaaaaatttt ttgttaaaat ccaatttcct caactcctcc aaactacgta
     3301 aatgatatag tctcataata aaataaaacc tctcagctaa tgttgttgaa attgtccact
     3361 aaacctaatt gatattgctt aggagataca cctgcgtact ttttgaatac tctcgaaaag
     3421 tgtgaagaat ctgaaaatcc taaacctaaa gaaacttcac tgatagtatg gtttgatttc
     3481 aacattattt tagccgtaga aatttttcgc ttagagatat aattataaat cgtaatatgc
     3541 atttcattct taaagctttc tcttaattta gtttcactta cagaaaaata attagctatt
     3601 tcctttacag taattctttt atagatattt aagccgatga attgaacgac agaagaaatt
     3661 aagggagaaa gatgctctat cttagttttt ccgatttcct tagtataata aacaatccca
     3721 gaatccctca cttttaagac ctcaggtaac gatacagcta attcgttttc ttgtatcaaa
     3781 gcatctctac tttgatacgc ttctattata tccattccta atgttataga cgtttgtgct
     3841 aacttttcaa aaacaatgat agaataattt ttttcagacc gaatcgtatc tccactaaca
     3901 cttggaatta ttccattact cagattaaaa accatgtttt ctaaatttcg aatatctcca
     3961 tttttaacat attccagtat tttattttca taaagaaaca gatatatttc tggatcgtaa
     4021 ttttgtgata acagattatc tattttgatt tgctcgatag ttttacttag attttctgta
     4081 tattcaatta acggttcatg gaatagatct tctatttttc cagtaaaaaa ataatgaact
     4141 aaaatcagaa tatctcgaat gtctcctaaa gaatacaaag tcaaattttg caatgaatta
     4201 tagagtcgtt ctctatcagc cattgcaaca ttcaaattgt tcatttttag tttaaaaaaa
     4261 actttatcaa tattatttgc tctaaatgga ccaaataagt aatatctggt tttaaacatg
     4321 tatagaagaa acatctcatt aaaatcacca gtaaagaaat ggaaatcttt agttttttta
     4381 atattatcag ttagtaactt tttat
//
LOCUS       DACGLE010000128         4304 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0534
            SAMEA3304449-rid9560453.denovo.135, whole genome shotgun sequence.
ACCESSION   DACGLE010000128 DACGLE010000000
VERSION     DACGLE010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304449
            Sequence Read Archive: ERR985064
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4304)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4304)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702225.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702225.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 20:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,045
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 27 of 67
            Pseudo Genes (incomplete)         :: 25 of 67
            Pseudo Genes (internal stop)      :: 19 of 67
            Pseudo Genes (multiple problems)  :: 4 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4304
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304449-rid9560453.denovo.135"
                     /strain="VREN0534"
                     /db_xref="taxon:1352"
     gene            147..1136
                     /locus_tag="IXT20_002702"
     CDS             147..1136
                     /locus_tag="IXT20_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="HAQ4861105.1"
                     /translation="MKKKKQDLINGTFREKKKNLKKVVAFSDNRISPKMRELMTGCGS
                     YLEFIATIDKEKKKLVQAHFCKNRFCPLCAWRKARKDSMMLSIMMQAIAEEKQYQFIF
                     MTLTTPNVKAECLNDEIDLFNHALKKMFMRKRVKTSIKGYVRKLEITYNKKRNDYNPH
                     FHLILAVNKSYFTDKDYYIKQSEWLDLWREVTGKTGINPDGTDEITQLDIRKVKGFQQ
                     EKAVQEVAKYSAKDFELTESQEVFDTFYHAMKGRQLITFNGVFKEYKKKYESGDLDRY
                     KKKDENEYFWFLTASWMRKESKYAVDYREMSADERNYFSGMYVDEKSVEGENE"
     gene            1133..1552
                     /locus_tag="IXT20_002703"
     CDS             1133..1552
                     /locus_tag="IXT20_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4861106.1"
                     /translation="MTDSTDKMTIKALADELGITKNTVRNYLVKAGYDLARYKKNGII
                     LLDKELIGVARSIYRSKGGQLTGHLPVNSTGSDFPLEKENEFLRDQLAEKDKHISELT
                     EMIRDQQKLTLLQQQKNDQLLLENQELKNRKWWQFWK"
     gene            1561..1869
                     /locus_tag="IXT20_002704"
     CDS             1561..1869
                     /locus_tag="IXT20_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4861107.1"
                     /translation="MDKIDLREETVNRIEVMYERLADYLNLSDEDNYSEMLERMMDVL
                     NSVINADLAKSSVLTNEFVHKHLAQTTDEAEKEFWLKLMSHIMQLNQISVINSKEKGM
                     "
     gene            1873..2235
                     /locus_tag="IXT20_002705"
     CDS             1873..2235
                     /locus_tag="IXT20_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4861108.1"
                     /translation="MGMTLADIKVQEEKLKKEQAKLRQQKKRLIEKENAAMGKALRKR
                     FGFESFADFERWLSSISDDQQHEKSVGNELRADEKSAPSERNYPNESDGIFGGIPNRK
                     SQMSESGFASMFSDVNSR"
     gene            2457..3863
                     /locus_tag="IXT20_002706"
     CDS             2457..3863
                     /locus_tag="IXT20_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase"
                     /protein_id="HAQ4861109.1"
                     /translation="MGFSISFKKGTKNTSISHNNRELTDSQKINDWHKHIDFTKSDEN
                     IYLEQINIREKYEELFGEAVEKYNAKQKRADRKIEDYLAKVRKDKKMEPQREFIVQIG
                     TLDDFRTTRDDGSSTGISEQQAEQNRVIANKILVEYFKEFKERNPSLSVYNAVIHNDE
                     VSPHLHLNIVPVAEGYKRGVQKQPSFNKALQQQGLRADKENGRVIWNNFRNQEVESIE
                     RLMADFGWERELVGTNKIKDIHEYKEIMSEISELRQTAVKERESVSDELRYICRQREE
                     NEREKALVASERAKIEEEKKNMLNVKKEMAVIFQKDMVPLESFKRLPDGSYHLSGNDF
                     VDLYQRATRATNAKRNADNMSEQYLQMLEQNTELNEYVAALEEERHLSGRKQLADLQV
                     ENKKLKEENRSLRQRLERLKNAFQKSITRLSIRFGLAEKDMGLSEELEVLEQEKTLFY
                     RQNVSQHKLERSEDEIEW"
BASE COUNT         1496 a          602 c          996 g         1210 t
ORIGIN      
        1 aaaaaaaaga ttgctttttc ttccctagta ttttatagtt ttaattgact agaaaaaaac
       61 taaactagaa aggagagcaa tctatttttt gttatcggct tttcagcctt ttttgtatac
      121 aaaatttgta gattcagtat agcaagatga aaaagaaaaa gcaagatttg attaacggaa
      181 catttcgaga aaaaaagaaa aatttgaaaa aagtggtggc atttagtgat aatcgaattt
      241 ctcccaaaat gcgtgaattg atgacaggct gtggttcata tttagaattt attgcaacga
      301 ttgataagga gaaaaagaaa ttggtccaag ctcacttttg taaaaatcgt ttctgtccgt
      361 tatgtgcttg gcgtaaagct cgtaaggatt cgatgatgtt gtcgattatg atgcaagcta
      421 ttgcggaaga aaagcagtat caatttattt ttatgacgtt gacgacaccg aatgtaaaag
      481 cggaatgttt gaacgatgaa attgatttgt ttaatcatgc attgaaaaag atgtttatgc
      541 gcaaacgggt taagacttcg attaaaggct atgtgcgaaa attggaaatc acgtacaaca
      601 aaaaacggaa tgactataac ccgcattttc acttgatttt ggcagtaaat aaatcttatt
      661 tcacggataa agattattat atcaagcaat cggaatggtt agatttgtgg cgggaagtga
      721 ccggaaaaac gggaattaat ccggatggta ccgacgaaat tacccagttg gatattcgca
      781 aggtcaaagg gttccaacaa gaaaaagcag tacaggaagt tgcgaaatat tcggcaaaag
      841 attttgaact gaccgaaagt caggaagtgt ttgacacatt ctatcatgca atgaaaggtc
      901 gtcagttgat tacgtttaac ggtgtattca aagaatacaa gaaaaaatat gagtcgggtg
      961 accttgatcg ttataagaaa aaagatgaga atgaatattt ttggtttttg actgcttcgt
     1021 ggatgaggaa agaatcgaaa tatgcggttg attatcgtga gatgagtgct gatgaaagaa
     1081 attatttttc aggaatgtat gtcgatgaaa aatctgttga gggggaaaat gaatgactga
     1141 ttcaacggat aaaatgacaa ttaaggcgct tgctgatgaa ctaggaataa ccaaaaatac
     1201 agtgagaaat tatctagtca aagccggata tgatcttgct cggtacaaga aaaatggaat
     1261 tatcttgcta gacaaagagt tgattggtgt tgctcggtca atttatcggt caaaaggtgg
     1321 tcaattgact ggtcatttac cggtcaatag cacgggcagt gattttcctt tggaaaaaga
     1381 gaatgagttt ttgcgtgatc aattagctga gaaggacaaa catatttctg aattaacaga
     1441 aatgattcgt gatcaacaaa aattgaccct gcttcagcaa caaaaaaacg atcagttgtt
     1501 actagaaaat caagaattaa aaaatcgaaa atggtggcaa ttctggaagt gagatgtgac
     1561 atggataaaa ttgatttgag agaagaaaca gtcaatagaa ttgaagtgat gtatgaacgg
     1621 ttagctgatt atttgaatct ttcggatgaa gataattata gtgagatgct tgaacgaatg
     1681 atggatgttc tcaactcggt tatcaatgct gatctagcaa aatcttcagt tttgactaat
     1741 gaatttgttc ataagcattt agcgcaaaca actgatgaag ctgaaaaaga attttggttg
     1801 aaattaatga gtcatattat gcagttaaac caaatttccg ttattaattc caaagaaaag
     1861 gggatgtagt ttatgggaat gactttagca gatattaaag tgcaagaaga aaaattaaaa
     1921 aaagaacagg cgaaacttcg ccaacagaag aaacgattga ttgagaaaga aaatgctgca
     1981 atgggcaagg ctcttcgcaa gcgttttggg tttgaatctt ttgctgattt tgagcggtgg
     2041 ctatcgtcga tttctgacga tcagcaacac gaaaaatcag tgggtaatga gttgcgagct
     2101 gatgaaaaat cagcaccaag cgaacggaat taccctaacg aatctgacgg aatattcgga
     2161 gggataccga atagaaaaag tcagatgagt gagagtggat ttgcgtcaat gtttagtgac
     2221 gtaaattcac gataagcgaa tattttgcat gaagcaaaat aaagcgtaaa aggagttgag
     2281 agcgtgacac gctttcggct ccttttttcg ttgtcacttg tgacggcgaa aaccctcgga
     2341 gagcgtttgg aattgtgaca gtcatgtcac aattaggaaa acgttactct ttgacaaaga
     2401 gtaacagaaa ggtttacact ctttttatta gcgacagcgc gaaggagggg tttaacttgg
     2461 ggtttagtat ttcgttcaaa aagggaacaa agaatacaag catttctcat aataatcgtg
     2521 agttaactga tagccaaaaa atcaatgact ggcataagca tattgatttc acaaaatcgg
     2581 atgaaaatat ctatttggaa caaataaata ttcgtgaaaa atatgaagaa ttattcggtg
     2641 aagccgtaga aaaatataat gcaaagcaga aaagagccga tagaaaaatt gaggactatt
     2701 tagcaaaggt tcgcaaggat aaaaaaatgg aaccacagcg agaatttatc gtgcaaattg
     2761 gtactcttga tgattttcgt actactcgtg atgatggttc ttctactggc atatcggaac
     2821 aacaagcaga acaaaatcga gtgattgcca acaaaatttt agttgagtat ttcaaagaat
     2881 ttaaagaacg gaatcctagt ttatccgtct acaatgctgt tattcataat gacgaagtaa
     2941 gtccgcacct gcatttgaat attgttcctg ttgctgaagg gtataaacgg ggtgttcaaa
     3001 agcaaccaag ttttaacaaa gccttgcaac agcagggttt acgagcagat aaagagaatg
     3061 gtcgagtgat ttggaataat tttaggaatc aggaagttga gtcaattgaa cgtttgatgg
     3121 cagattttgg ttgggagcgt gagttagtcg gtactaacaa aatcaaagat attcatgaat
     3181 acaaagaaat catgagcgaa atttccgaat tgcgtcaaac ggccgtaaaa gagcgtgaga
     3241 gcgtttctga tgagttgagg tatatttgta ggcaaagaga agaaaacgag cgtgagaagg
     3301 ctctggtggc ttctgagaga gcgaagattg aagaagagaa gaaaaatatg ctgaatgtaa
     3361 aaaaagagat ggcggtcatt ttccaaaaag atatggttcc tttagaaagt ttcaagaggc
     3421 ttcctgatgg ttcgtatcat ttatctggga atgattttgt tgatttgtat caacgagcta
     3481 cgagagcaac aaatgccaag agaaatgctg ataacatgag tgagcaatat ttacagatgc
     3541 ttgagcaaaa tacagaatta aatgagtatg tagctgcatt ggaagaagag cgtcatttgt
     3601 ctggtagaaa acaattagca gatttacaag tggaaaataa aaagctgaaa gaagaaaatc
     3661 gctctttacg ccagcgttta gagcgattaa aaaacgcatt tcaaaaatcg atcactcgtt
     3721 tgtctatccg ttttgggctt gcagaaaagg atatggggct ttctgaggaa ttagaagtat
     3781 tagagcagga gaaaacctta ttttatcgtc aaaacgtctc acagcataaa ttagagcgtt
     3841 ctgaagatga gatcgagtgg tagttacgat gtgcagtcaa cggtaccgga ggcggtttgt
     3901 gccgaggata aggagttgac ggctaaacca ctagcctatt aagctgacat ttgctaaaaa
     3961 atggcaagtc cattttttgg ccaacttagc gttagcgaag tttgggggcg ccctttttaa
     4021 agggtgcggt tttatgtttg catgactaag aatgagtggg aaaatttaac agccctatct
     4081 aacaggcggt taaatgtaca aaatatgtac acccccttaa aaatgcagtg gtcgattgat
     4141 cgaattttat gtctttgggt tttggggttt taaaacgaaa aaaagggggc gattttcgcc
     4201 ctcttttttg atgttttttc ttatcttgat actataggca actatttctg cgaagaaatc
     4261 ggggctatag tatgggtcta tcccttgtgg ctctaaggaa gaac
//
LOCUS       DACGLE010000129         4037 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0534
            SAMEA3304449-rid9560453.denovo.136, whole genome shotgun sequence.
ACCESSION   DACGLE010000129 DACGLE010000000
VERSION     DACGLE010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304449
            Sequence Read Archive: ERR985064
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4037)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4037)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702225.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702225.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 20:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,045
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 27 of 67
            Pseudo Genes (incomplete)         :: 25 of 67
            Pseudo Genes (internal stop)      :: 19 of 67
            Pseudo Genes (multiple problems)  :: 4 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4037
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304449-rid9560453.denovo.136"
                     /strain="VREN0534"
                     /db_xref="taxon:1352"
     gene            179..775
                     /locus_tag="IXT20_002707"
     CDS             179..775
                     /locus_tag="IXT20_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HAQ4861110.1"
                     /translation="MNIAASRKLINFISIIGLGLSIALTIYFINLGVFKDLNALRGLV
                     GDSIILGPIIFVFIQILQVVIPIIPGGISTAAGVLIFGPYAGFIYNYIGICIGSIIIF
                     LLGRRYGKPFILSMISDKTYNKYIGWLDNQNRFEKLFALAIFLPVAPDDALCLMAGLT
                     NMSVKRYTLIILIAKPLSIFLYSMALIYGGQYLSGLLG"
     gene            899..2698
                     /locus_tag="IXT20_002708"
     CDS             899..2698
                     /locus_tag="IXT20_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HAQ4861111.1"
                     /translation="MNYLKRSLGNAWDFLKGTQAYFRDVLLMHGFILFICLPLLSSTT
                     RFILHRGAIDYLSYDNIPSIFSQHPGVLLSLIVVLMTILLLVYFEFTFLLMSVFFIKK
                     KEPISLKQLLHLTILQLKKVRPITFLFFLAYFLLILPISGLSFNSDLLSKIKIPAFIM
                     DFIFANRWIIVSSFLLVYIFLGYIGIRLIFALPEMILRDRPFRAAIRESWSLTKSRLL
                     AITGQFIVIGGTILLLSSLGYIVVILAQSMVEQFFPDYALISAVFAMTLLQGILLFNI
                     VMSTVGIFYIIVDFMDDEGFLPEIPKWFIPQAPNLRFSALKNTGLTLFAVFFGIGVCL
                     YNMDYLTSAVQTKPVTVSHRGVSTQNGAQNTLEALEKTSRDYHPDYVEMDVQETKDGH
                     FVVMHDANLRHLTGVNGTPQDLTLKELTNLTVTENGVQAPVCSFDSYLEKAHKLNQKL
                     LIEIKTSKKDSDDLVERFVHKYRKVILENHHILQSLTYQTVSDLKEAEPNFYVGYILP
                     FNIVGPPVTPADFLTMEYSTINRNFINSAHQDGKEVYVWTINDSDAMSRMMFYGVDGI
                     ITDEMGLLNESVKQTDEEITYSDKLLNFVLGVG"
     gene            complement(2976..3188)
                     /locus_tag="IXT20_002709"
     CDS             complement(2976..3188)
                     /locus_tag="IXT20_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4861112.1"
                     /translation="MVKAEVGRSYICKPIGVSHELIGCIERTYINTAVVYVESYDQRD
                     YQLINESKYRMLVKFCDISAPAELVS"
BASE COUNT         1186 a          688 c          711 g         1452 t
ORIGIN      
        1 tttaaaaaaa acaagttatt aaataagtct ttagttggat gtttttttga gatcaatctt
       61 ttatacttat gatgaactta caaaattgtt tgttttctgt catatcaaaa aatggcgcag
      121 ttagttgctt ttctagtaaa gtatagttaa caaagaacct ctaaggagtt gatgaataat
      181 gaatattgct gcctctcgaa aactgataaa tttcatatcc attatcggat taggcctatc
      241 aatcgcgctg acgatttatt ttattaattt aggtgttttc aaagatttga atgcgttaag
      301 aggattagta ggtgattcca tcattttagg accgattatt tttgtcttca tccaaatcct
      361 gcaagtagtt attccaatca ttcctggagg gatcagtact gcagctggtg tcctgatatt
      421 tggcccttat gctggattta tctataacta tattggaatc tgcattggtt cgattatcat
      481 ttttctgcta gggcgtcgtt atggaaagcc attcatactt tccatgatta gcgataagac
      541 atataacaaa tatattggct ggctggataa tcaaaaccgt tttgagaagc tgtttgctct
      601 agctattttt cttccagttg ctcctgacga tgcgttatgt ttgatggctg gattgacaaa
      661 catgtcagtg aaacgatata cactaatcat tttgattgct aaaccactgt cgattttctt
      721 atatagtatg gccttgattt atggaggtca atatctaagt ggtcttcttg gataatattt
      781 attttgtaaa attagtcatt gattgacaat gttaaatctg ggaaagggtc tgcaaaacac
      841 actgcaccct ttcctttttc attagatttg cgctataatg tgaagagcag gtgataggat
      901 gaattatttg aaacgtagct taggtaatgc ttgggacttt ttaaaaggaa cacaagctta
      961 tttccgtgat gtcttgctga tgcatggctt tattttattt atttgtttac ccttattgag
     1021 cagtacgacc cgttttattt tacaccgagg ggcaattgac tatttgtctt atgacaatat
     1081 tccttcaatt ttttctcaac atcctggggt tctactctcg ttaatcgtag ttttgatgac
     1141 gattttactg ttagtttatt tcgaatttac ttttcttttg atgagtgttt tttttattaa
     1201 aaagaaagaa ccaatctctt tgaagcaact cttacacttg acgatacttc agctgaagaa
     1261 agtgagaccc attacttttt tgtttttcct tgcttatttt ttattgattc taccgatcag
     1321 cggattaagt tttaattctg atctactgtc aaaaataaaa atccccgctt tcattatgga
     1381 ttttattttt gctaatcgtt ggatcatcgt cagttccttt ttactcgttt atattttcct
     1441 tggctatatt gggattcgac tgatttttgc tttaccggaa atgattctac gggatcgtcc
     1501 ttttcgagca gccattcggg aaagctggtc attgaccaaa tcgcgtcttt tggccatcac
     1561 gggccaattt attgtgattg gcggtacgat tttactcctt tcttctttag gatatatcgt
     1621 tgttattttg gcacaaagta tggttgaaca atttttccca gactatgcat tgatcagcgc
     1681 tgtgtttgct atgacgcttt tacaagggat tttattattc aatatcgtga tgtctaccgt
     1741 agggattttt tatatcattg ttgatttcat ggatgacgaa ggcttcttgc ctgagatccc
     1801 taagtggttt atcccacaag cccccaactt gcgtttctct gcgctgaaaa acactggttt
     1861 gactcttttt gcagtttttt tcggtattgg cgtctgtctt tacaatatgg attatctgac
     1921 ttcagctgtt cagacaaaac cagtgactgt ctctcacaga ggtgtgagta cccaaaatgg
     1981 ggcgcaaaat actttagaag cacttgaaaa aacaagcaga gactatcatc ctgattatgt
     2041 agagatggat gttcaggaaa caaaagatgg tcatttcgta gtgatgcatg atgcaaatct
     2101 tcgtcactta acaggggtaa atggaacacc acaggatctt acattgaaag aattgacaaa
     2161 tcttacagtt actgaaaacg gagtgcaagc ccctgtttgt tcttttgatt cttatttgga
     2221 aaaagcacat aagctaaacc aaaaattact tattgagatc aagacctcta aaaaagattc
     2281 ggatgacctt gtcgaacgat ttgtccataa ataccgcaaa gttatcttag agaaccatca
     2341 tattttgcaa agtttgacgt accaaaccgt ttcagattta aaagaagcag aaccaaactt
     2401 ttatgtgggc tacatcttac ctttcaatat tgttgggccc cctgtgacgc ctgctgattt
     2461 tctgacaatg gaatatagta cgatcaaccg taacttcatt aattctgccc accaagacgg
     2521 aaaagaagta tatgtatgga caatcaacga ttcagatgct atgagtcgca tgatgtttta
     2581 tggtgtggat ggtatcataa ccgatgagat gggactacta aatgaaagtg tgaaacaaac
     2641 agatgaagaa atcacttatt cagataaact gctaaatttt gttttgggag tcggctaatc
     2701 aaaaatcgtt aatgaaaaaa gtctttatac aacaaaaaac gcataacctg ccaccgcagg
     2761 tatatgcgtt ttttgtttga cgctcataat gtgtgcttaa ctttctcttg attccttcat
     2821 cgcacgaaca atagctaaaa tagcaggaat tgcagagatc actgcaagaa taattcccat
     2881 tttccctgaa ctttttcttt ttggcatatc ttctcgctcc cttctgtctt tatttatatt
     2941 ataagggagt tcttgcgaat tctcaaaaat actgtttaag aaaccaattc agcaggtgcg
     3001 ctaatatcac agaattttac gagcatgcga tacttgcttt cattgataag ttgatagtct
     3061 cgttggtcat aactttcaac atacacgaca gccgtgttga tatatgtacg ttcaatacag
     3121 ccgattagtt catgagatac tccgatcggt ttgcatatat aagatcttcc tacttctgct
     3181 tttaccattt cattccctct tttctttatt tttattttgt tttttcttta ttttataata
     3241 acaaattacc ttgtatttga aaacgttttt atccattttt ttatttttta ttttgagtat
     3301 ctttctgctt tttagataac tattttttaa cacgatctta gacgttgtga caaaaacgtt
     3361 tgtttatatt cctatgttgt tcgtgcgttt ttttctttat attgctaagt tatttataaa
     3421 gaggtgagca tatgatttat attcaagcaa tagtatttgt aaccttgatg attgggctac
     3481 ttatcggatt aaaagaaaat agctaaacca tttaagaaaa aataaataag gaaaatgata
     3541 aatgcttttt aattgcatca aaaaacgtac gatgctaaac ttggtataag ttcgaaagtt
     3601 taaaggaaac tttcaagaac taaattacat tacaaaggag atgaatttta tgacgtttac
     3661 aaaaacaaat atgcaatacc gcgtagcttt tgatacaact tctaaccgtt ttatggcgat
     3721 tgatgcacaa aacgaaaata acgttgcgtt tggtattaca atcgaacaag cagtccgagc
     3781 attaaacgaa tctagataag gagtgaacca ttccaaatgg cgactgatga gatctcggaa
     3841 aatcaataaa agacgtttgt aacgttaatc cgcgacaaac gtctttttgt attcattaca
     3901 tatatttgga aattgtctct ctttatctct agacctaaac aaaaacacaa gttctaatat
     3961 caaccatctc gttttttatg acaattttgt tagctgtttc gatgataata ttccgttatt
     4021 atctgtataa tatttaa
//
LOCUS       DACGLE010000130         3980 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0534
            SAMEA3304449-rid9560453.denovo.137, whole genome shotgun sequence.
ACCESSION   DACGLE010000130 DACGLE010000000
VERSION     DACGLE010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304449
            Sequence Read Archive: ERR985064
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3980)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3980)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702225.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702225.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 20:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,045
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 27 of 67
            Pseudo Genes (incomplete)         :: 25 of 67
            Pseudo Genes (internal stop)      :: 19 of 67
            Pseudo Genes (multiple problems)  :: 4 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3980
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304449-rid9560453.denovo.137"
                     /strain="VREN0534"
                     /db_xref="taxon:1352"
     gene            482..1807
                     /locus_tag="IXT20_002710"
     CDS             482..1807
                     /locus_tag="IXT20_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HAQ4861113.1"
                     /translation="MFDYSREPVNDYFLIDMKSFYASVECIERNLDPMTTPLIVMSRS
                     DNTGSGLTLASSPTAKKKYHITNVSRATDLPYPLPKELHIVPPRMNLYIKKNMEVNQI
                     FRKYVADEDLLIYSIDESILKVTKSLNLFTSSGTRSERRRELAKIIKDEIQKETGLIA
                     TVGIGDNPLLAKLALDNEAKSSKEFIAEWTYETVPEKIWKINEMTDFWGIGSRMKKRL
                     NHMGIMSIEDLANWNPYTIKSQLGVIGLQLYFHTNGIDRTDIAIPPEPTKEKSYGNSQ
                     VLPKDYTRREEVELVVKEMAEQVAIRIRQHNCKTGCVRLAIGTSILEEKSGFSHQMKI
                     PITDNTKELQNYCLFLFNKFYEGQEVRHIGVTYSRLVYTDSLQLDLFSDPRKKIAEEN
                     LDRIIDKIRQKYGFTAIIHASSMLEGARSITRSTLIGGHAGGLGGLHND"
     gene            1800..2147
                     /locus_tag="IXT20_002711"
     CDS             1800..2147
                     /locus_tag="IXT20_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4861114.1"
                     /translation="MIEYNDRGRLKWSGFYLSDHTERINADNVQRANHNLAKEQMTTE
                     EIAKILRTAVIKNRPVCIQKEERNAEGYYPDDVVGKIIGYDELGVFVEEEKVHYDEIR
                     NVSFSELLKWSQI"
     gene            2498..3319
                     /locus_tag="IXT20_002712"
     CDS             2498..3319
                     /locus_tag="IXT20_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HAQ4861115.1"
                     /translation="MGLLVPRANTYTIPLTKRQLIAKRYQRITRAINREFWNSESDTA
                     HSLYVGSYGRGTAISTSDIDIIVELPMAEFDRFKNYLSNGPSKLLQVIKNAFQEILPN
                     SDIRADGQVVKINFHDGIKFEIVPAFNEKDYWGESKGFIYPDSNMGGNWKATNPKKEQ
                     EAMKLKNTKSNNLLYATCKHFRHVRDTEFTSYHLSGIVIDSFVYEAMGNWKFVENNSG
                     GQNISSVSYETALLEYYNSHKVMGGLNLYSPGSNQFVNSDSSIICLEKVLKKIAL"
     gene            3339..3899
                     /locus_tag="IXT20_002713"
     CDS             3339..3899
                     /locus_tag="IXT20_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLATT domain-containing protein"
                     /protein_id="HAQ4861116.1"
                     /translation="MGDEKKRLEVQLREAYGRVTYTYTTHLKQADNIISDNKILRCLQ
                     IGLSALTTGGVLSTIITDNSELTFVAGLCSTISLGLNLYFKNFDLAEEAKNHQIVADK
                     LWLIREEYVSLLTDLNRLSVEDIVKKRDQLMNETHEAYKNAPKTNSKSYESAQKALKS
                     EEEQFFTEEELDKMLPEHLRKKSSKY"
BASE COUNT         1515 a          523 c          740 g         1202 t
ORIGIN      
        1 ctaatcgaag taaaacccaa agccatatga acccctgaat cagcgaaagt ttatgatgat
       61 ttggattact aatagtttag gaattagatg gtaaatctaa attaaacaat cgttgatttg
      121 gcaacgaaat agtgagggaa aatccactca ctgaagggta aactccgtca agttatgtga
      181 ccagacggaa ggacgaatgt tgttgaggtt ttagaaccat ataaaacagc atagttctca
      241 aattgtttaa aatgattgtc aagttaaaaa aatctgggat catataaaat agtatagttc
      301 tcaaatatga ttagaaaccc tattaaagag caatattaag ccaacttact taaatcgttt
      361 aattaagaac aataatctct tttagtcaga cttttagtct gacttttttg tatttccaaa
      421 atttattctt tacaaaaacg aacatacgtt tgtataattg cttgtgaatg ggggaataaa
      481 aatgtttgat tacagtagag aaccggtgaa tgactatttt ttaatcgaca tgaagtcttt
      541 ctatgctagc gtggaatgta ttgaacgaaa tttagaccca atgactacac cgttaattgt
      601 catgagtaga tcggacaaca ctggttcagg tctgactcta gcttcttcac ctactgcaaa
      661 gaaaaaatat catattacta atgttagtag agctactgat ttaccttatc cattaccaaa
      721 agaacttcat attgttccac cacgaatgaa cttatatata aaaaaaaata tggaagttaa
      781 tcagattttt agaaagtatg tcgcagatga agatttattg atttattcga ttgatgaatc
      841 aattttaaaa gtgacaaaat ccttaaattt atttactagt tcaggaacaa gaagcgagag
      901 aagaagagaa ttagctaaaa taatcaaaga tgagattcag aaagaaacag gtctaatagc
      961 aacagtcgga ataggagata acccattatt agctaaactt gcactggaca atgaagcgaa
     1021 gagtagtaaa gaatttatag ctgagtggac ttatgagact gtacctgaaa aaatatggaa
     1081 aataaacgag atgactgact tttggggcat tggatcacgc atgaaaaaaa gattgaatca
     1141 tatgggaatt atgagtatag aagatttagc aaattggaat ccctacacaa ttaaaagtca
     1201 gttaggtgta atcggactcc agttatattt ccataccaat ggaattgatc gtacggatat
     1261 tgctattcca ccagaaccaa ccaaagaaaa gtcttatggg aattcacaag ttctaccaaa
     1321 agattatacg aggagagaag aagtagaact agtagtcaag gagatggcag agcaagtagc
     1381 cattcgaatt agacaacaca attgcaaaac tggttgtgta cgcttagcaa ttggtacgtc
     1441 aattttggaa gaaaaatcag gtttctcgca ccagatgaaa ataccaatta cagataatac
     1501 gaaagaatta caaaattatt gtcttttttt attcaataag ttttatgagg ggcaggaagt
     1561 acgacatata ggagtaactt atagcagatt agtatacaca gattccctac aattagattt
     1621 atttagtgat ccaagaaaaa agattgctga agaaaatttg gataggatta ttgataagat
     1681 tagacaaaaa tatggattta cagctattat acatgcttca tctatgttag aaggtgcaag
     1741 aagtattacg agaagcactt taataggagg acacgcagga ggattaggag gtttgcataa
     1801 tgattgaata taacgataga ggacgtttaa aatggtcggg attttatttg tctgatcata
     1861 ctgaaagaat caatgcagac aatgtacaac gtgctaatca taatttggca aaagaacaaa
     1921 tgacgactga agagattgcg aaaatactga gaactgctgt cataaaaaat agacccgtct
     1981 gcattcaaaa agaggaacga aacgctgaag gttactatcc agatgatgta gtaggaaaaa
     2041 ttattggata tgacgagctt ggtgtatttg tggaagaaga aaaagttcac tatgatgaaa
     2101 ttaggaatgt ttctttctcg gaattattaa aatggagtca gatatgaaca ttattaaaaa
     2161 atacgaattc ggttatatta cgtatgaaca gctaatagag gaattatggg gatatggtca
     2221 acaattaatt aatgaagtgg ggattgattg cttttgtttc tatattgaat ccggagcagg
     2281 ataccataag aacagatact atatatttcc atatccacca aaatagtctc ttagatattt
     2341 ttaaattttc tccaaatact ttcaattatt gtgaaatatt tccctttaat taatcaaatt
     2401 ttttataaaa gtaatatata aaataaattg cttgaagtat tatatttaga tatcataatt
     2461 tatggaagaa aaattaatta aaaaggatga gattataatg gggttactgg ttccgagagc
     2521 taatacttat actatacctt taacgaaacg ccaattaatt gctaaaagat atcaaaggat
     2581 tactagagcc atcaatagag agttttggaa ttctgaaagt gatactgctc acagtttata
     2641 cgtaggttct tatgggagag gaacggcaat tagtactagt gatatagata taatagttga
     2701 attaccgatg gcagaatttg acagattcaa aaattattta tcaaatggcc cgtctaagtt
     2761 attacaagtt ataaaaaacg catttcagga aatactacca aatagtgata ttagagctga
     2821 tggacaagtt gttaaaataa atttccatga tggaatcaaa tttgaaatag ttccagcttt
     2881 taatgaaaaa gattattggg gtgagtcaaa agggtttatt tatccagatt caaacatggg
     2941 agggaactgg aaagctacaa atcccaaaaa agagcaagaa gccatgaaat taaagaatac
     3001 aaaaagtaat aatttattat atgctacgtg taaacatttt cgacatgtac gagatacaga
     3061 atttacgagt tatcatcttt caggaatagt aattgacagt ttcgtatatg aggctatggg
     3121 aaattggaaa ttcgttgaaa ataacagtgg gggtcaaaac attagttctg tatcttatga
     3181 gacagcttta ttagaatact ataactcaca taaagttatg ggcggtttaa atttatactc
     3241 tcctggaagc aatcaatttg ttaattctga tagtagtatc atatgtttag aaaaagtatt
     3301 aaaaaaaata gctttataaa gttttatgga ggaacaacat gggagacgaa aaaaagcgct
     3361 tggaagttca attacgtgaa gcatatggac gagtaacgta tacttacacc actcatttaa
     3421 agcaagccga taatattatt tctgacaata aaattttaag gtgtcttcaa attggtttgt
     3481 cagcccttac tactggaggg gtacttagta ccattattac tgataatagt gagttaacct
     3541 ttgttgctgg attatgttca acaatttctc ttggattaaa cttatacttt aagaattttg
     3601 atcttgctga agaagctaaa aatcatcaaa ttgttgccga taaattatgg ttgatcagag
     3661 aagaatacgt aagcttacta acagatttaa atcgtttaag tgttgaagat attgtgaaaa
     3721 aaagagatca attaatgaac gaaactcatg aagcatataa aaatgcccct aaaacaaata
     3781 gtaaaagcta tgaatcagct cagaaagcat taaaatctga agaagagcaa ttttttacag
     3841 aagaggaatt ggataagatg ttaccagagc atcttagaaa aaagagttct aaatattgaa
     3901 ctcttttttt atgcttaaaa ttctcttgta atttttctat tcatctatat ttaagaagaa
     3961 atatgatgat agaaacatct
//
LOCUS       DACGLE010000131         3923 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0534
            SAMEA3304449-rid9560453.denovo.140, whole genome shotgun sequence.
ACCESSION   DACGLE010000131 DACGLE010000000
VERSION     DACGLE010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304449
            Sequence Read Archive: ERR985064
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3923)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3923)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702225.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702225.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 20:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,045
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 27 of 67
            Pseudo Genes (incomplete)         :: 25 of 67
            Pseudo Genes (internal stop)      :: 19 of 67
            Pseudo Genes (multiple problems)  :: 4 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3923
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304449-rid9560453.denovo.140"
                     /strain="VREN0534"
                     /db_xref="taxon:1352"
     gene            complement(573..1130)
                     /locus_tag="IXT20_002714"
     CDS             complement(573..1130)
                     /locus_tag="IXT20_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4861117.1"
                     /translation="MRNLSNRNKILIIIVVIAVFHLGTNAVLSRIILGPKPPRPEITR
                     GEFDFRLEYEVDGERIVIEDTIVALFDGFSADAGSMAWYRTWRLHLASDRRSRNILLD
                     ELEDGRRIYYVPESANYSMGDVQKKREPNPNWYPFNGVTIEYPRNKTPEIGAKFISGL
                     EDLYDRFGIRLISWEHDPPIENRFE"
     gene            complement(1309..1920)
                     /locus_tag="IXT20_002715"
     CDS             complement(1309..1920)
                     /locus_tag="IXT20_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4861118.1"
                     /translation="MNSNRGNKMEYNGSMRIVQIIFWITSGIVIIGGVFLMLPSLIFP
                     FFALISPKIPEPEITYGEFPFRIEYELDGQINIIEDTVIAEFNGCEFSAGSMKRERRW
                     RSRLASGREDLPFGDDLGIYFSRGSAQYYMGENVQSMSLKPHIALRNLEEGFKREVDF
                     ILGESGYIRTVLNFGEAQEVLTQYGITLINWEISEPIVNNFGD"
     gene            complement(2033..2290)
                     /locus_tag="IXT20_002716"
     CDS             complement(2033..2290)
                     /locus_tag="IXT20_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4861119.1"
                     /translation="MRAVHVNPTIEITMIGHSKGGAEAANSVAINTNAIIFNPATVNL
                     SAYELDSNTYTANMVAYVVEGDALNVVFGGVYTYWRINHVR"
     gene            complement(2887..3165)
                     /locus_tag="IXT20_002717"
     CDS             complement(2887..3165)
                     /locus_tag="IXT20_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4861120.1"
                     /translation="MEKHNITGSMYVDDQWFISTDEVKIAAIRRDYLQKIHKIKDPNG
                     KKARLIITLITSNGKKLKLAGSYDNVKKFEHWYLQKDSISLNKQFEKN"
     gene            complement(3199..3450)
                     /locus_tag="IXT20_002718"
     CDS             complement(3199..3450)
                     /locus_tag="IXT20_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4861121.1"
                     /translation="MKEKPINKWSLYKNSMFGVVIFGVCAGLFLFILLQTVTLFSSNP
                     TYGWRDSIALGMFAGGYGFLLLFSILTLSPIHGLIQLWK"
BASE COUNT         1194 a          799 c          520 g         1410 t
ORIGIN      
        1 ttattccgaa taaatggtgt ccaaaagctt gcatctgcca gattatctca tttcttatga
       61 caagctaagc aaattatctc caccaaataa tttattcata tttttgactt gcgcatacta
      121 actaattctt aaattttcat acaaaatttc tcaatttccc ttaacgagta atatttatgt
      181 acggtgattc tcactagtat cccaatcact ttttaagtta tcccgatttc aattctactt
      241 caaaaatagt tgcttttatg tatgctgtta gttgattaga tggtcatttg ttgagctagg
      301 tcgtttattt taaacaagtt gtttctttag cttataccta tctcgtctac caaacgcaag
      361 atggcaaaca caaatgcata cgcatctgta tttgccactt tgattcgtat aaagctaaca
      421 actattaact cctctcagct aatagttgct ctctttttta ttcagattca tttttgctga
      481 tcacagccta gtatttcaat ggttttgaac actaaaccaa attgttccat tacaacattc
      541 actggctgat gaaatggatt agtagatttc gattattcaa agcgattttc tattggtgga
      601 tcgtgttccc aactaataag acggattcca aacctatcgt acagatcttc taaaccactt
      661 ataaattttg cacctatttc aggtgtttta ttgcgtggat attctattgt tacgccatta
      721 aatggatacc aattaggatt aggctctctt tttttctgta catctcccat agaatagtta
      781 gcactttctg ggacataata tattcttcga ccatcctcta attcatctaa taagatattt
      841 cgtgatcgtc tatcacttgc taaatgtaga cgccacgtgc gataccatgc catgctacct
      901 gcatctgcag aaaatccatc aaataatgca acaattgtat cttctatcac aatcctttcc
      961 ccatctactt catattctaa tctaaaatca aactcccctc gggtaatttc tggtcttggt
     1021 ggcttaggac ccaatattat tctacttaaa accgcattcg ttcccagatg aaacacagct
     1081 ataacaacaa taatgattaa tattttgttt ctatttgata aatttctcat tattcgcaca
     1141 tcacctctac attctcacat attagttcgt aatactcctt taatatcaaa atatctactg
     1201 gtcgtaataa tattcgctat gaattcctac tactcgtata agaaaggcca aatcatcagc
     1261 ttttacttgc tgatgatttg gcttgtttct atttatcgta attgttttct aatcaccaaa
     1321 attgttaaca atcggttcag atatttccca gttgattaag gtaattccgt attgtgtcaa
     1381 aacttcttgc gcttctccga aattaagtac agttcttatg tatccacttt ctcctaatat
     1441 aaagtctacc tctcttttaa aaccttcctc gagatttcta agggctatat gtggttttaa
     1501 tgacattgat tgtacatttt cccccatata atattgcgca cttcctcgac taaaatagat
     1561 tcctagatcg tccccaaaag gtagatcttc tctaccacta gctaatctac ttctccatct
     1621 tcgttctctt ttcatagaac ctgcgctgaa ctcacacccg ttaaactcag ctatgacagt
     1681 atcttcaatt atgttaatct gtccatctag ttcgtattca atcctaaatg gaaattctcc
     1741 ataggtgatt tccggttcgg gtattttagg tgagattaat gcaaagaaag gaaatattaa
     1801 gcttggcaac atcaaaaaaa ctcctcctat gatcacaatt cccgatgtaa tccaaaatat
     1861 aatttgtaca attctcattg atccattgta ttccatttta ttccccctat tactattcat
     1921 atccgcctat ctatatcgat cttccaatgc agagataatt gcttccatac cttgattttt
     1981 tactgagtta cctatgcgag atatcattct ccactaaggt gcatcatatt gtctatctaa
     2041 catgattaat tctccaatag gtgtatactc ctccaaaaac aacatttaat gcatctccct
     2101 cgacaacata agctaccatg tttgctgtat atgtgtttga gtctaattca taagcagata
     2161 aatttactgt cgctggatta aatataatcg cattcgtatt tatagctact gaatttgctg
     2221 cttcagcacc accttttgaa tgtccgatca ttgtgatttc aatagtagga tttacgtgga
     2281 cagccctcac cgtttaccct gaccattata gaaatcgctt acttttttga actacgcaac
     2341 aaaggataca atgctcctaa cttcactgtc tccatttttg tccactaagg aatataaaaa
     2401 ataggcatta attcatttaa ttcggtcaca acacgttacc ttttaaatca ttatactgta
     2461 tctcatacat tattatcaat aaactattat aaaaatgcaa aaaatgcttt atcaatctat
     2521 tttgtaattg gtcataatat cattgaaatc ataccctgta tcgatttttc ctatgaatct
     2581 cgtctatttg tgattgacga aaaagaggca agacagacac tggaatggta attttaccca
     2641 ctattttgct ccaactattt gtaatcgtac ccacttttga aataccgaga tgatcatttt
     2701 aattgatatg ctgattccca agcaaaaccc caagaaatta aatgctctat cgcatctagc
     2761 ttcttgggtt ttgcttatat aaattccaac atttaaattt tttatgctgt tcggtctcag
     2821 aaatgccgtt tttgcttata tcacaatatc aagatgtcaa attattcgtg ttgccgatga
     2881 ctttgattaa tttttttcaa attgtttatt aagactaatg ctgtcttttt gcagatacca
     2941 atgttcgaac tttttaacat tatcatatga tcctgcaagt tttaacttct taccattaga
     3001 tgttatcaaa gttataatta accttgcctt tttcccatta ggatctttta ttttatgaat
     3061 tttctgcagg taatctctcc taatggcagc tatttttacc tcatcagtgc tgataaacca
     3121 ttgatcatcc acatacatac tgcctgttat gttgtgtttt tccatttctt ccgagaacga
     3181 aaagtttaag gctcgttctt acttccaaag ttgaatgagt ccatggattg gacttagtgt
     3241 gagaattgag aacaacaata agaatccata tcctcccgca aacatcccta aggctatact
     3301 gtctctccat ccataagtag gattagaaga gaaaagagtc accgtttgta ataatatgaa
     3361 aaggaataat ccagcacaaa caccaaagat aacaactcca aacattgaat ttttatacaa
     3421 tgaccattta tttataggct tttctttcat tttacttccc cctactgttc tgttcacacg
     3481 gaatgaattc aaagttcata agccctcctc tttgaacata tgaatacgac ttgctaacta
     3541 tactttaaaa aagcttgttt taagcggtta taggcgagtt gtaatctcta ttcatctcat
     3601 tagcaaataa cttgccgaaa agtctactga aattaaagca gatagtggaa gaattattta
     3661 gccaggatag acactatttg tgaaacacat gtctttactt ttgaaattct atgatgaaaa
     3721 gccttggaat acagtagctc ttaattggta atactttttc cattataaat aacctctttt
     3781 cattttatat ttatcccctc taaaaataat atctaatcta atattttaaa cttatatttc
     3841 cctcgctgtg ctaatagagc attactaaat aaatagaaac tagaaaaaag cattacgcct
     3901 gccagaataa accaattatt tgt
//
LOCUS       DACGLE010000132         3873 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0534
            SAMEA3304449-rid9560453.denovo.141, whole genome shotgun sequence.
ACCESSION   DACGLE010000132 DACGLE010000000
VERSION     DACGLE010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304449
            Sequence Read Archive: ERR985064
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3873)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3873)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702225.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702225.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 20:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,045
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 27 of 67
            Pseudo Genes (incomplete)         :: 25 of 67
            Pseudo Genes (internal stop)      :: 19 of 67
            Pseudo Genes (multiple problems)  :: 4 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3873
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304449-rid9560453.denovo.141"
                     /strain="VREN0534"
                     /db_xref="taxon:1352"
     gene            154..309
                     /locus_tag="IXT20_002719"
                     /pseudo
     CDS             154..309
                     /locus_tag="IXT20_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392734.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="(4Fe-4S)-binding protein"
     gene            328..1605
                     /locus_tag="IXT20_002720"
     CDS             328..1605
                     /locus_tag="IXT20_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ4861122.1"
                     /translation="MIKNAIAYITRKKNRTLIIFIILTIVLSCLYSCLTIMKSSNEIE
                     KALYESSNSSISITKKDGKYFNVNQFKDIEKLKEIEEKIIQYDGLAKLKDAKVVSGEQ
                     RINREDLSDEFKNVVSLEATNNTKRNILFSSGVFTIKEGKNIEENDKNSIIVHEEFAK
                     QNNLKLGDEVDLELLDIEKSGKIKSHKFKIIGIFSGKKQETYTGLSSDFSENMVFVDY
                     STSQEILNNSENNEIANKILMYSGSAESTDLALNKLKELKIDESKYFVEKDSNAFEES
                     LESVSGIKYIIKVMTYSIMLGGMVVLSLILILWLRERIYEIGIFLSIGTSKIQIIMQF
                     IFELIFISIPSIISSLFLGNVLLKVIVDGFINSEDSMISGGSLINNSSFMSNITTLGQ
                     SYLILISIIVLSVVFASSLMLIKKPKEILSKIS"
     gene            1615..2271
                     /locus_tag="IXT20_002721"
     CDS             1615..2271
                     /locus_tag="IXT20_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004612033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ4861123.1"
                     /translation="MDILEIKNVAYSYANSKEKVLSGVNQKFELGKFYAIVGKSGTGK
                     STLLSLLAGLDKPQTGKILFKNEDIQNKGYSNHRKNNISLVFQNYNLIDYLSPIENIR
                     LVNKSADESILFELGLDKKQIKRNVMKLSGGQQQRVAIARALVSDAPIILADEPTGNL
                     DSVTAGEIINILKTLAKDRNKCVIVVTHSKEVADSADIILELSGKKLKKVNKMNLEVE
                     "
     gene            2271..3647
                     /locus_tag="IXT20_002722"
     CDS             2271..3647
                     /locus_tag="IXT20_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ4861124.1"
                     /translation="MIKNAFAYVTRKSLKSLIIILVILSMSTLSLISLSIKDATDRAS
                     KETFANITNSFSMEINRQVNPGTPRGGGNVKGEDIKKISQTDSIDSYVKRINSVADLV
                     DYDIIETQETLANQSPERAKNFKRTVMLTGVNDSTKETKFVSEAYKLVEGKHLENEDK
                     NKILMHKDLAEKNNLKVGDKIKIKSNLFDADNEKGADETVEVEIKGLFDGHNSGGVSA
                     AQELYENTLITDIHTAAKVYGNTEDTAVYQDATFFVKGDKNLDSVIKDLGKLDINWRE
                     YNLIKSSSNYPALQQSISGIYSISNKLFFGSLIFAGVVVSLLLFLWMNARKKEIAVLL
                     SLGISKLEIFGQFLIEMVFISIPAFVGSYFLAQYTADKLGNNILNKVTGDIAKQIAKQ
                     SASSQLGGGAEAEGFNKTLSSLDINVLPKFMIYVVIFMSLVLLVSLIISSFNILRRNP
                     KELLIDNN"
BASE COUNT         1546 a          410 c          674 g         1243 t
ORIGIN      
        1 aggcttacca gcgagataga gatcgacgat ttgttgtttg aattcttttg aataggttct
       61 tcgttgacgt cttggcatga aaaatcctcc agtgtgtttt ttattattct acacacctta
      121 ttttttctgt ctagtctagt gtagccgatt caggataagg ataagtgtat taattgtgga
      181 aaatgtaaaa aagtttgcca aatgaataaa gattcagttg agaattgcaa tcatctggag
      241 tgtatcagat gtggacgatg taaaaatgct tgtccagtgg atgcaattag ttatggagta
      301 agaaaataga aaggagtagt taggaaagtg ataaaaaatg ctatagcata tattacaaga
      361 aagaaaaata gaactttaat aatttttatc atattaacta tagttctttc ttgcttgtat
      421 tcttgtttaa caataatgaa atcaagcaat gaaatagaaa aggctttata tgaaagttct
      481 aattcttcaa tatcaataac aaaaaaagat ggtaaatatt ttaatgttaa tcaatttaaa
      541 gatattgaaa aattaaaaga aattgaagaa aaaataattc aatatgatgg attagcaaaa
      601 ctaaaagatg ctaaagtagt tagcggagaa caaagaataa atagagaaga tttatctgac
      661 gaatttaaga atgttgtttc actcgaagct acaaataata ctaaaagaaa tattttattt
      721 agtagtggag tatttacaat taaagaaggt aaaaatatag aagaaaatga taagaattca
      781 attattgttc atgaagaatt tgctaaacaa aacaatctaa aattaggtga tgaagttgat
      841 cttgaattac tagatattga aaaaagtgga aaaataaaaa gtcataaatt taaaattata
      901 gggatctttt ctggtaaaaa acaggaaaca tatacaggat tatcttctga ttttagtgaa
      961 aatatggtgt ttgtagatta ttcaacaagc caagagatat taaacaattc agaaaataat
     1021 gaaattgcaa ataaaatttt aatgtattct ggtagtgcag aatctacaga cttagcctta
     1081 aacaaattga aagagcttaa aattgatgag tcaaagtatt ttgttgaaaa agattctaat
     1141 gcgtttgaag agtctttaga gtcagtgagt ggaataaaat atataataaa agtaatgact
     1201 tattctatta tgttgggtgg gatggttgtt ctttcattaa tcttgattct atggttaagg
     1261 gaaagaattt atgaaatagg tatattctta tctattggaa catctaagat acaaattata
     1321 atgcaattta tattcgagtt aatattcata tcgataccaa gtataatatc ttccttattt
     1381 ttaggaaatg tattactaaa agtaattgta gatggattca ttaactcaga ggattcaatg
     1441 atttccggtg gaagtttaat aaataatagt agttttatgt caaatataac aacacttgga
     1501 caaagttatt taatattaat aagtattatt gttttatcag ttgtatttgc ttcttcatta
     1561 atgttaatca agaagcctaa agaaatctta tcaaaaataa gttaggagaa aataatggat
     1621 atattagaaa taaagaatgt agcatatagt tatgcaaatt ctaaagaaaa agttttgtca
     1681 ggagtaaatc aaaaatttga acttgggaag ttttatgcga tagtaggaaa gtcaggaaca
     1741 ggaaaatcta cacttctttc cttacttgct ggacttgata aacctcaaac aggaaaaata
     1801 ttgtttaaga atgaagatat acaaaataaa ggatatagta atcatagaaa aaataatata
     1861 tctttagtat ttcaaaacta taatttaata gattatttat caccaattga aaatattaga
     1921 ttagtaaata aatcagcaga tgaaagtatc ttgtttgaac taggtttaga taaaaaacaa
     1981 ataaaaagaa atgttatgaa attatctggt ggacagcaac aaagagtagc tattgctaga
     2041 gcattggtat cagatgctcc aataatacta gctgatgagc ctactggtaa tctagacagt
     2101 gttactgctg gagaaataat taatatatta aagacattag ctaaagatag aaataaatgt
     2161 gtaatagttg taacacatag taaggaagta gcagattctg cggatatcat tttagaacta
     2221 agtggtaaaa agttgaaaaa agtaaataaa atgaatttgg aggttgaata atgataaaaa
     2281 atgcatttgc ttatgtaact agaaaaagtt taaaatcatt aattatcata ttagttattt
     2341 tatctatgtc aactttaagt ctcattagtt tatctattaa agatgctacg gacagagctt
     2401 caaaagaaac atttgctaat ataacaaata gtttttctat ggagataaat agacaagtaa
     2461 atccaggaac acctagaggt gggggaaatg taaaaggtga agatattaaa aagatatctc
     2521 aaacagatag tatagactcc tatgtaaaaa gaataaacag tgttgcagat ttagttgatt
     2581 atgatattat tgaaactcaa gagactcttg cgaatcaatc acctgaaaga gcaaagaatt
     2641 ttaaaagaac agttatgtta actggggtta acgactcaac aaaagaaaca aaatttgtat
     2701 cagaagcata taaattagta gaaggaaagc atttagaaaa tgaagataaa aataaaatct
     2761 taatgcataa agatttagct gaaaaaaata accttaaagt tggagataag ataaaaataa
     2821 aatctaattt atttgatgct gataatgaga aaggtgcaga tgaaacagta gaagtagaaa
     2881 ttaaaggtct attcgatggg cataatagcg gtggagtaag tgcagcacaa gaactatacg
     2941 aaaatacatt aatcactgat attcatacag ctgctaaagt ttatggtaat accgaagata
     3001 cagctgtata ccaagatgca acattctttg taaaaggtga taagaatctt gatagtgtaa
     3061 taaaagatct tgggaaatta gatataaatt ggagagaata taatttgatt aaaagttcat
     3121 caaattaccc tgcattacaa caatctatat caggtattta ctcaatttca aataaattat
     3181 tttttggctc attaatattt gcaggagttg tagtttcatt attattattc ttatggatga
     3241 atgctagaaa gaaagaaata gcagtattac tatcattagg tatatcaaaa ttagaaattt
     3301 ttggtcaatt cctaattgag atggtattta tatcaatccc cgcattcgtt ggttcctatt
     3361 tcttagctca atatacagct gacaagcttg ggaataatat attgaataag gttactggtg
     3421 atatagctaa acaaatagct aaacaatctg catctagcca attaggtggt ggggcagaag
     3481 ctgaaggatt taacaagaca ttatcaagtt tagatatcaa tgtattacct aaattcatga
     3541 tttatgtagt tatatttatg agtttagtac ttcttgtatc attgattatt tcttcattca
     3601 atatattaag aaggaatcca aaagaattat taattgacaa taattaaaat tttcggtgct
     3661 ttttagcacc aaaaatgaga gtgtaatata aaccgtgtaa gtaaccatca atcctaggcg
     3721 ggtggttgct tcacggtttt tatttttcta aaataagatc aagaggtgat tacacgatgg
     3781 ctagaaagaa aagaaatcct gatgctgaga agttagctga atccattttg aatacttatc
     3841 aacccgaatc tgtagaggat atgcaagatg ttt
//
LOCUS       DACGLE010000133         3539 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0534
            SAMEA3304449-rid9560453.denovo.143, whole genome shotgun sequence.
ACCESSION   DACGLE010000133 DACGLE010000000
VERSION     DACGLE010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304449
            Sequence Read Archive: ERR985064
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3539)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3539)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702225.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702225.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 20:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,045
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 27 of 67
            Pseudo Genes (incomplete)         :: 25 of 67
            Pseudo Genes (internal stop)      :: 19 of 67
            Pseudo Genes (multiple problems)  :: 4 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3539
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304449-rid9560453.denovo.143"
                     /strain="VREN0534"
                     /db_xref="taxon:1352"
     gene            complement(<1..268)
                     /locus_tag="IXT20_002723"
     CDS             complement(<1..268)
                     /locus_tag="IXT20_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HAQ4861125.1"
                     /translation="MRKIVIATHSMMAKGLKDTLEFIIGPQKSLLAITAYIDSDYSIN
                     QEIEQLFSDLAPDDELIVTVDLLGGSVSNAFSEQIGKDNFYLISG"
     gene            complement(279..1292)
                     /locus_tag="IXT20_002724"
     CDS             complement(279..1292)
                     /locus_tag="IXT20_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009141002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAQ4861126.1"
                     /translation="MLGFSEERFLSVLNGAVKNIETINQVADAISEKGFKNLYLVGSG
                     GTYATISPLAYLLSTHSELDYYHEIAAEVVCSQPKKINKDTVLITSSLTGTTQETVDV
                     VKYANSQGATTIAFVGNAATPLGEIVDYAIENDADNDTLVEELQLQFFALGARLMKNN
                     GEFPEYDEFIQTLQKMPEVLLSVRQQNDQKALEFAEKHKNTEFHMFVGAGNTYGAAYE
                     YAMCVLEEMQWIATKSIHAAEFFHGTIEMTDKSMSFVLLKGEDQTRPLVDRVERFVNK
                     YSDVVTVFDTADFELAGVPSAMRQYVAPMVMATALERVSAHFEKVRDHSLDIRRYYRT
                     VEY"
     gene            1509..2243
                     /locus_tag="IXT20_002725"
     CDS             1509..2243
                     /locus_tag="IXT20_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ4861127.1"
                     /translation="MNTEELTNKLIQDIEQEKYKYAQAIPSERDFVQIYEMNRSAVKK
                     AIQQLVNKEYLLKIHGKGTFVKKNIHEHLQIKFHGLSELLEREGIQPSSLILASEIKP
                     AGHIVGKALQLEPTEEVFHLVRLRSGDGEPISIEDTYIPLNLIPDLQAFDFQIFSFYS
                     TLAAYHYQIENIYQTISSSKVRNKEARLLKTSLNRPVISLAITAVTTNEQAVEYTEVV
                     VLPEFAAIYTKNVHQGKEMKIYAQID"
     gene            2227..2925
                     /locus_tag="IXT20_002726"
     CDS             2227..2925
                     /locus_tag="IXT20_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ4861128.1"
                     /translation="MPKLINLLAYDLIDKIIAYIHEHHLTPGTKLPSERDLSEMFNVN
                     RTTLRGALQKLVDNGTLRAQTNSGYYLTAPKFEKNAHHFFTPIEDSLLSKQRLQLKAT
                     ALSPKKAAALCPELNDFKQQQPQAYLEYIDAEAISLMLLIPMETLKKSNFSDNPFQDF
                     KSLAYTRSQEISLITDNDVAADLLGVDKEEVLLFVREEIRAGKELAAIALGYTISRRC
                     NIQVNVATTLQSHS"
BASE COUNT         1121 a          721 c          638 g         1059 t
ORIGIN      
        1 caccgctgat taaataaaaa ttgtctttac cgatttgttc ggagaaagcg ttagacacgc
       61 ttcctcccag taaatctacc gtcacaatta attcgtcatc aggcgctaag tcggaaaata
      121 gctgctctat ttcctggtta atggagtagt cactatctat atacgcagtt attgctaaaa
      181 gtgatttttg aggaccaata ataaattcta aagtatcctt taagccttta gccatcatgg
      241 aatgcgtcgc aattacaatt ttcctcatag ctcttacctt aatattccac agtccgataa
      301 taacgacgaa tatctagtga atgatcccgc accttttcaa aatgagcact cactcgttct
      361 aaggccgttg ccataaccat tggcgctaca tactgacgca tagcgcttgg tacacctgct
      421 aattcgaaat ccgcagtgtc aaaaaccgtc acaacatcgc tatatttatt tacaaaacgc
      481 tctacgcgat cgactagcgg acgcgtttga tcttcacctt ttaataacac aaaactcata
      541 gatttatcag tcatttcaat agtcccatgg aaaaattcgg ctgcatggat cgacttggtg
      601 gcaatccatt gcatttcttc cagtacgcac atagcatatt cataggcagc cccataagta
      661 ttcccagctc caacaaacat atgaaattcc gtatttttat gtttttctgc aaattcaagt
      721 gctttctgat cattttgttg ccgaacactt agtaatactt ccggcatttt ttgtaatgtt
      781 tgaataaatt catcatattc aggaaactca ccgttatttt tcatcaggcg tgcacccaaa
      841 gcaaaaaact gtaactgaag ttcttctact aatgtatcgt tgtcagcatc attttcaatc
      901 gcataatcca cgatttcccc gagtggtgta gcagcatttc ctacaaacgc aatcgtcgtt
      961 gcaccttgac tattggcata cttaacaaca tcaacggttt cttgagtcgt accggttaat
     1021 gaagatgtga ttaacactgt atctttatta atctttttcg gctgtgaaca aaccacctct
     1081 gcggctattt catgataata atcaagctct gaatgagtgc ttaacagata agctagcggg
     1141 ctgattgtgg catatgttcc accagagcct accaaataaa gatttttaaa tcccttctca
     1201 ctgattgcat ctgccacctg attgattgtc tcgatatttt ttactgcacc atttaatact
     1261 gatagaaaac gttcttcgct aaaacctaac atagttaaaa acctccaagt caataatttg
     1321 aaaaatatat ttgcaaatgc cggcacatta acaaaccact taatcttttt tatataccac
     1381 ctgttaacgt ttttaatgta ccacttatag aaaacgctgt caatagtttt taaccacttt
     1441 tatttttatt tgtaccaaaa tccttttttc atgtataata aacaaaaata gactctggag
     1501 tgtaacgtat gaatactgaa gaactaacga acaaattaat acaagacatt gaacaagaaa
     1561 aatataaata cgcgcaagct attccttcag aacgtgattt tgttcaaatt tatgaaatga
     1621 atcgttcagc cgtaaaaaaa gccattcaac aattagtaaa taaagagtac ctactaaaaa
     1681 tacatggcaa aggaactttt gttaaaaaaa atatccatga acatttgcaa atcaaatttc
     1741 atggtttgtc tgaattgttg gaacgggaag ggattcagcc tagcagctta attttagctt
     1801 cagaaataaa accggctgga cacattgtcg ggaaagcttt acagctggaa ccaactgaag
     1861 aagtatttca tttggttcgt ttaagaagcg gcgacggtga gccaatttct attgaagaca
     1921 cttatattcc attaaatctc atacctgatc ttcaagcttt tgatttccaa attttttcat
     1981 tttattccac attggctgct tatcattatc aaattgaaaa tatctatcag acaatttcca
     2041 gctcaaaagt gcgcaataaa gaagcccgtt tgttaaaaac cagcttaaat cgtccggtaa
     2101 tcagtcttgc tatcactgcg gttacgacaa atgaacaagc ggttgaatac actgaagtag
     2161 tggttttgcc tgaattcgcc gccatctata ctaaaaatgt tcatcaagga aaggagatga
     2221 aaatctatgc ccaaattgat taatctgttg gcttatgacc tgattgacaa aattattgct
     2281 tatattcatg aacatcattt aacccccgga acaaaactgc cttcggaacg ggacctatca
     2341 gaaatgttta atgttaatcg aactacctta agaggcgcgt tacaaaaact ggtggacaac
     2401 ggcaccttac gggcgcaaac caatagcggc tattatttaa ctgcgccaaa attcgaaaaa
     2461 aatgcccatc atttctttac accgatagaa gattctctgc ttagtaagca gcggctacag
     2521 ctaaaggcaa cggctctttc accgaaaaaa gcagcagctc tttgtccgga actgaatgat
     2581 ttcaaacagc agcaaccaca agcttattta gaatatattg atgcagaagc aattagcttg
     2641 atgttattaa tccctatgga aacattaaag aaaagtaatt tctcggacaa tcctttccaa
     2701 gattttaaaa gtttggcata tacgcgttct caagaaattt ctttaataac tgacaatgac
     2761 gttgcggcgg acttgctggg agttgacaaa gaagaggttc tgttatttgt acgagaggaa
     2821 ataagagccg gtaaggagtt ggcggcgatt gctttaggtt atactattag tcgccgctgc
     2881 aacatacagg ttaatgttgc tacgaccttg caatcccata gttaggaaaa actattcgct
     2941 gtaagttcaa ccgtttcaat gcaagcgaat cacaaatcaa aaagtattta actcgtgttt
     3001 attagaaata gtcatgaact tttagtctcc aagatcaaac cgacagcgtt caactctgag
     3061 aagcaaaagt aaatgtacga tcaagtatga ggctgggaca gaagcgctaa gctccgagaa
     3121 ccaagagaag gtacgttcat tatcgaatca ctgcattctt cgtgtttatc agcaatttcg
     3181 gcttatttcc gcaggccttc aagaaggttg aagcaaaagt cgttcagatt tgagcaattg
     3241 gaggaaaagg cgctccagac gttccttgtc tggagcggct gttatgaaat ggccgaagat
     3301 ctgacttttg accaccgttt aatcaaggtt gagccaaaag tcgttcaggt ttgagcaatt
     3361 ggagtagaag acaaaacaga tgttttttgt ctggttggct gttacgaaat tgccgaagag
     3421 gctgattttt aaccaccgtt tatctagtca tagcacttag agattgatgc gatcgacgcg
     3481 tagctagagt ggctgccgct gcaatgaccc gtggcaaaat atactgcttt ttcattttt
//
LOCUS       DACGLE010000134         3528 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0534
            SAMEA3304449-rid9560453.denovo.144, whole genome shotgun sequence.
ACCESSION   DACGLE010000134 DACGLE010000000
VERSION     DACGLE010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304449
            Sequence Read Archive: ERR985064
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3528)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3528)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702225.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702225.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 20:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,045
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 27 of 67
            Pseudo Genes (incomplete)         :: 25 of 67
            Pseudo Genes (internal stop)      :: 19 of 67
            Pseudo Genes (multiple problems)  :: 4 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3528
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304449-rid9560453.denovo.144"
                     /strain="VREN0534"
                     /db_xref="taxon:1352"
     gene            89..562
                     /locus_tag="IXT20_002727"
     CDS             89..562
                     /locus_tag="IXT20_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="topology modulation family protein"
                     /protein_id="HAQ4861129.1"
                     /translation="MGRENRPSQGRSKKSPAFRIQAKKWFLQEQQSFMVSNPSWIVEG
                     NYTSTLEERIKEADKIIWLKVPRYLAIYRVVWRSLKRKINKKSRPDMPESFSEKLNRK
                     YLEFLSFIWHFEGNNEPKIQQLIQVANARNKLIILKTRREKQEFLTKLKIESKNN"
     gene            636..1076
                     /locus_tag="IXT20_002728"
     CDS             636..1076
                     /locus_tag="IXT20_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopY/TcrY family copper transport repressor"
                     /protein_id="HAQ4861130.1"
                     /translation="MEKVKTPVKISDSEWEIMRVIWTLGQTTAQEITQILADSMDWKP
                     ATIKTLLGRLVKKEVIWTEQEGKKFIYHPAVSEMETVRSATENLFSHICAKRIGETIA
                     DLVEEATLTQEDINEILKQLEQKQPVDTIECNCIPGQCECKQHQ"
     gene            1089..1298
                     /gene="copZ"
                     /locus_tag="IXT20_002729"
     CDS             1089..1298
                     /gene="copZ"
                     /locus_tag="IXT20_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone CopZ"
                     /protein_id="HAQ4861131.1"
                     /translation="MKQQFSIEGMSCNHCAARVEETVSALDGVQKVKVNLKKANGTVK
                     FDETKVQSEKICQAINGLGYKAEVI"
     gene            1298..3484
                     /locus_tag="IXT20_002730"
     CDS             1298..3484
                     /locus_tag="IXT20_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HAQ4861132.1"
                     /translation="MATAETKMDTFVITGMTCANCSARIEKELNGQPGVVNATVNLAT
                     EKASVKYEGTTTEKLIQSVENIGYGAILYDEAHKQKIAEEKQAYLKKMLFDLILSTVL
                     TLPLMLSMIAMMLGSHAAIVHFFHFPIVQLVLSAPVQFYVGARFYKGAYHAIKSKAPN
                     MDVLVAIGTSAAFALSIYNGFFRGHPQDLYFESSSMIITLILLGKYLEHTAKTKTGNA
                     IKQLMSLQTKTAQVIRNGKEETLAIEEVVVGDQLVIRPGEQIPADGRIISGSSAIDES
                     MLTGENLPVEKNPDDTLFGGTINTNGLLHMEVTQVGKQTVLAQIIQMVEDAQGSKAPI
                     QKIADRISGIFVPIVLVIAFITLIATGLITGDWQLALIHSVSVLVIACPCALGLATPT
                     AIMVGTGVGARNGILIKGGEALEAAAHLDSIVLDKTGTITEGKPKVTDLVGSKEVLSI
                     FYTLEQASEHPLGKAIVEYGKLQEAATYDMIDFTAHPGAGISGTINGVRYFAGTRKRL
                     IELNLSFDEYQEHALELEQQGKTVMFLADEKQVIGLIAVADQIKLEAKQAIKQLQNKG
                     LDVFMLTGDNKLAAETIGKQVGIDPKHIFAEVLPEDKAAYVEKLQKDGKKVGMAGDGI
                     NDAPALALADVGMAMGSGTDIAMETADVTLMSSSLASIAQTIELSRVTLRKIKQNLFW
                     AFVYNTIGIPFAALGFLNPIIAGGAMAFSSVSVLLNSLSLNRHMKK"
BASE COUNT         1213 a          603 c          813 g          899 t
ORIGIN      
        1 tttttttgcg gcgttataag tggtatgatc gaaccaaatt ccaaagagaa gtgagggaga
       61 agaatgaaga tcatcgtgat tggacaaagt gggtcgggaa aatcgtccct cgcaaggaag
      121 atcaaagaaa tcaccggctt tccgcattca ggcaaaaaag tggtttttgc aagaacagca
      181 atcgtttatg gtatctaatc cgtcatggat cgttgaagga aattatactt ctacgttaga
      241 ggaaaggatt aaagaagcag ataagatcat ttggcttaaa gtgccgcgtt atctcgccat
      301 ctaccgagta gtttggcgtt ctctaaaaag aaagataaac aaaaaatctc ggccagatat
      361 gcctgaatca ttttcggaga aactaaacag gaaatactta gaatttttat cttttatctg
      421 gcattttgaa ggaaacaatg aaccaaaaat acagcagctg atccaagtag ctaatgcaag
      481 aaataaactg atcatcctta aaacaagacg agagaaacaa gaatttttaa ctaaattgaa
      541 aatagagagc aaaaataatt gacagttttt gtttacaagt gtaatctgta ttcataagtt
      601 aatgattaca tttgtaaacg aaaggtgtga aataaatgga aaaagtcaaa acgccagtaa
      661 agattagtga ttcagagtgg gagatcatgc gtgttatttg gacacttgga cagacgacag
      721 cccaagaaat cacgcagatc ttagctgatt cgatggattg gaaaccagca acgatcaaaa
      781 ctttgctggg tcgtttggta aaaaaagaag tgatctggac agaacaagaa ggaaaaaaat
      841 tcatttatca tccagctgtc tcggagatgg aaacagttcg cagtgcgacg gaaaatcttt
      901 tttcccatat ctgtgcaaaa aggattggag agacgattgc ggatctagta gaagaagcca
      961 ctttgactca agaagatatc aatgagattt tgaaacaact ggaacaaaag caaccagtag
     1021 atacgatcga atgtaactgt atcccaggtc aatgtgaatg taaacaacat caataggagg
     1081 aagaaaaaat gaaacaacaa ttttcaatcg aaggtatgag ctgcaatcat tgtgcagcaa
     1141 gagtagaaga aacagtaagc gcacttgacg gcgtgcaaaa agtgaaagta aatttgaaaa
     1201 aagcaaacgg tacggtcaag tttgatgaaa caaaagttca atccgaaaag atttgtcaag
     1261 caatcaatgg attgggttat aaagcagagg tgatttgatg gcaacagcag aaaccaaaat
     1321 ggatacgttc gtcattacag gtatgacttg tgcaaattgt tctgctagga tcgaaaaaga
     1381 gctgaacgga caacctggtg tggtcaatgc cacagtcaac ttggccactg aaaaagcaag
     1441 tgtcaaatat gaagggacga ccactgaaaa attaatccaa agcgtagaaa acatcggcta
     1501 tggtgcaatt ttgtacgatg aagcccacaa acaaaaaatt gcagaagaaa agcaagcgta
     1561 tttgaaaaaa atgctattcg atcttatatt gagtacggtt ttgactttgc cgttgatgtt
     1621 gtcaatgatt gctatgatgt taggaagcca tgcggcgatt gttcactttt ttcattttcc
     1681 aatcgtccag ttagtgctgt ctgctcctgt ccaattttat gtcggtgctc gtttttataa
     1741 aggtgcgtac catgcaatca agtcgaaagc accaaatatg gatgtattgg tagcaattgg
     1801 aacatcggct gcctttgcat tgagcattta caacggattt ttccgtggac acccccaaga
     1861 tctttatttt gaaagcagca gtatgatcat cacgctgatt ttgctcggca aatatttgga
     1921 acatacagca aaaacgaaaa caggtaatgc aatcaaacaa ttgatgtctc ttcaaacaaa
     1981 aaccgcccaa gtgataagaa acgggaaaga agaaacgtta gcgattgaag aagtagtcgt
     2041 tggtgatcaa ctagtcatcc gtcctggtga gcagattcca gcagatggtc gaattatttc
     2101 aggaagcagt gcgatagatg aaagtatgct tactggtgaa aatcttcctg tagaaaagaa
     2161 tccggatgac acgctttttg gcgggacaat caatacgaat ggtctgctac acatggaagt
     2221 cacacaagtc ggcaagcaga cagtattagc gcaaatcatt caaatggtgg aagatgctca
     2281 agggagcaaa gccccaatcc aaaaaatcgc agatcggatc tcagggattt ttgttccgat
     2341 cgtattagtc atcgcgttta tcacgctcat agctacaggt ttgatcactg gtgattggca
     2401 actcgcattg atccatagtg tctctgtatt agtcattgcg tgtccatgtg cattaggttt
     2461 agcgactcca acagctatta tggtaggaac gggtgttggt gcacgaaatg gaattttaat
     2521 taaaggcgga gaagcattag aagcagctgc tcacttagac agcattgttt tggataaaac
     2581 tgggacgatt acagaaggaa agcccaaagt tacagatcta gtcggttcta aagaagtcct
     2641 atctattttt tatactttag aacaagcttc tgaacatcca ctaggtaaag caatcgtgga
     2701 atacggaaaa ctgcaagaag cagcaacata cgatatgatt gattttacag ctcacccagg
     2761 tgctggaatc agcggtacga ttaacggagt acgctatttt gcgggtacac gtaaacgatt
     2821 aattgaattg aacctatcat ttgatgaata ccaagaacac gcgttagaat tagaacagca
     2881 gggaaaaaca gtcatgtttt tagctgatga aaagcaagtg atcgggttga ttgccgtggc
     2941 tgatcaaatc aaactagaag ccaaacaagc aatcaagcag ctccagaata aaggattaga
     3001 tgtttttatg ctgacaggag acaacaaact ggcagccgaa acgattggta aacaagttgg
     3061 tattgatcca aagcatatct ttgctgaagt acttccagag gataaagcag cctacgtaga
     3121 aaaacttcag aaagatggca aaaaagtcgg tatggcaggc gatggaatca atgatgcacc
     3181 ggctttagca ttagctgatg ttggtatggc aatgggaagt ggaacggata tcgcgatgga
     3241 aacagccgat gtgacgctga tgagtagcag tttggcatcc attgcacaaa caatcgagtt
     3301 gtctagggtg acattgagaa aaatcaaaca aaatcttttc tgggcttttg tctataatac
     3361 gattggtatt ccttttgccg cactaggctt tttgaatcca atcattgcag gaggagcgat
     3421 ggcgtttagt tccgtcagtg tattgttgaa ctccctgagc ttaaatcggc acatgaaaaa
     3481 gtaagagaag ggatggagta aaaatgaatg ggaacgatat ggagaaaa
//
LOCUS       DACGLE010000135         3507 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0534
            SAMEA3304449-rid9560453.denovo.145, whole genome shotgun sequence.
ACCESSION   DACGLE010000135 DACGLE010000000
VERSION     DACGLE010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304449
            Sequence Read Archive: ERR985064
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3507)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3507)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702225.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702225.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 20:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,045
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 27 of 67
            Pseudo Genes (incomplete)         :: 25 of 67
            Pseudo Genes (internal stop)      :: 19 of 67
            Pseudo Genes (multiple problems)  :: 4 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3507
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304449-rid9560453.denovo.145"
                     /strain="VREN0534"
                     /db_xref="taxon:1352"
     gene            complement(<1..132)
                     /locus_tag="IXT20_002731"
     CDS             complement(<1..132)
                     /locus_tag="IXT20_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK-targeted surface antigen transcriptional
                     regulator"
                     /protein_id="HAQ4861133.1"
                     /translation="MFSDKEVLLKSLHVCLKLPIVVFNSEFSIIKEYRSDRTTYFFYD
                     "
     gene            826..1212
                     /locus_tag="IXT20_002732"
     CDS             826..1212
                     /locus_tag="IXT20_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAQ4861134.1"
                     /translation="MSDNDERVNAQLIRFFEKVILNTANTYYKKQEEIQKHEQLDQLP
                     PYLFTKDTIKFKQPINVLNVTLLVEDIKLAKILPLLKEPEQIFLIEKFIFDKTDKEIG
                     KVLGITRQGATNLKHRLYKKIQKMLV"
     gene            1306..1497
                     /locus_tag="IXT20_002733"
     CDS             1306..1497
                     /locus_tag="IXT20_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ4861135.1"
                     /translation="MNSLKEDFILAKAGNEEAVEAILKRFSSLIHKQSWRTGKYDQDC
                     YQECMLAIYLAISKFEIKE"
     gene            2011..2343
                     /locus_tag="IXT20_002734"
     CDS             2011..2343
                     /locus_tag="IXT20_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAQ4861136.1"
                     /translation="MRKTYDRKFKLKISQDILEKKVTTKKIAEEYNISRPTISRWVSE
                     YRRYGKNAFAGQGKRLPDKADFYILEQENKRLKEEDECSTNTKIDTDLQGSLKNFTMK
                     ESKSINVL"
     gene            2639..2794
                     /locus_tag="IXT20_002735"
     CDS             2639..2794
                     /locus_tag="IXT20_002735"
                     /inference="COORDINATES: protein motif:HMM:NF012870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HAQ4861137.1"
                     /translation="MPTVIHSDRGSQHRSFIYQELLNDHHITHSVSQTGTPVDNAVIE
                     AFHRSIK"
     gene            3070..3507
                     /locus_tag="IXT20_002736"
                     /pseudo
     CDS             3070..3507
                     /locus_tag="IXT20_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303667.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         1258 a          597 c          553 g         1099 t
ORIGIN      
        1 atcataaaaa aaataagtag ttctatctga gcgatactct tttataatac tgaattcgga
       61 attaaaaacc acgataggta actttaaaca aacatgaaga gactttaata agacttcttt
      121 atctgaaaac acttttgttt ctctctttcc tttctacatc attaaatagt attttatacg
      181 ttatctaaaa tactattcct ctgtcaaaat aagctaactt acttcattat aaaatatcca
      241 cttatcgctt tcatgtatat tccaccgtaa aaagtacatt ttatcaccga gaactcatca
      301 tttttcatca tgatgaatac tatttacata tatctagttc aaagacaata attattatga
      361 tccgttatta tctaagcact atacagataa taggaatttt tcgtttctaa atattccaat
      421 ttgggaatcc aataaaaatt ttcgttcaaa tacgtgtcta ttcgaactct tttctagttg
      481 ttaattatga agggggaaga aataaaaaat acaatctata cacgaataag aatattatcc
      541 aaaaggaggt tttagaaaag atttattata cgtaaaaagc tcaagacatt ttcttagcca
      601 taaggacatg tttgatattt aagaagatat atgaattttc ttttctaagt ataaagataa
      661 ctatccatta tcaactaaaa aaacattttt gaaaataaac atcagcagtc ctatcaatgc
      721 atgtgagctt atagaagtta ttcgcagcgc agcataattg cattagcttc ttttcatgtt
      781 ttttgcctct tttcccttta aatcttaatg gaggaggtgt gcaatatgtc agataacgac
      841 gaacgcgtaa atgcccagtt gattcgattc tttgaaaagg tcattttgaa caccgctaat
      901 acttattata aaaaacaaga agaaattcaa aaacatgaac aacttgatca actcccacca
      961 tatttgttta ctaaagatac tataaaattt aaacaaccga ttaatgtttt aaatgtgact
     1021 ctccttgttg aagatataaa attagcaaaa atccttcctt tgttgaaaga acctgaacaa
     1081 atttttctta tcgaaaaatt tatctttgat aaaactgata aagaaattgg aaaggttctg
     1141 gggattacaa gacagggtgc gacaaattta aaacatcgac tttacaaaaa aattcaaaaa
     1201 atgctggttt aatatctgca taataactaa ttttgtatgc ttctttgctc gttctcctcc
     1261 ttcccaaaga aatagattat aagtccatgg gaagaaggta gatttatgaa ttctttaaaa
     1321 gaagatttca ttttagcaaa ggctggaaat gaagaagctg ttgaggcaat tctcaaaaga
     1381 tttagctctt tgattcataa gcaatcctgg agaactggaa aatatgatca agattgttat
     1441 caagaatgta tgcttgcaat atatctagct atctcgaaat tcgaaattaa agaataactc
     1501 aactaaggga gtctgtgaca taagtaaaaa gatagtttca aatttcgaac aataaatttg
     1561 aaactatctt ctttataatt ataaagtatc gattttacag cgttctataa tgattcatag
     1621 aatctaaaac gaatgatttt aattatcaac gttttttaat tcgtttttag cttaaaaata
     1681 acttttgtca cagtctcact cctaaaatta ctttatttac gtatgaagtg ggcaggaggt
     1741 cgaatccaga actgggctat ggtacttcaa cgaggacttc tcctctagaa ttgaaaagca
     1801 tgcgatttac cttccgtaac gacggtagat ttattcaact atgtgtccaa tcaaaatttg
     1861 tctgatttca aaaatagtca agagaatttt ggttgttaaa aactcttatc tatcaacttc
     1921 cacagtttag ttgacaaacc ctgatactaa gttgggctat gatacacttt tcttgactat
     1981 cagttacgtt accggaagga atgaactcta ttgagaaaaa catatgatcg taaatttaaa
     2041 ctaaagattt cacaggatat ccttgagaaa aaagtaacta ccaagaaaat tgctgaagaa
     2101 tacaatatct ctcgtccaac aatttcccga tgggtttcag aatatcgtcg atacgggaaa
     2161 aatgcttttg ctggacaagg aaaaagatta cctgataaag cagatttcta cattcttgaa
     2221 caggaaaata aacgactaaa agaggaagac gagtgttcta cgaacacaaa gatcgatacg
     2281 gatctccagg gatcactcaa aaactttacg atgaaggaat cgaaatcaat aaacgtgttg
     2341 tagctgttct tatgcgtgaa atgaatttat gcacaagagg atttcatcga cgcaaatctt
     2401 ctcatggtag agaaaaaaca atcaaagaaa tggtaaaaaa atgttgaatc gataatttga
     2461 acagaaccaa attgatgctg tttgggtgat ggatattact tacattccat gtaatgacgg
     2521 tcgcctctat ttgtccacct atattgactt agcaactcgt attctacgtt actatatggt
     2581 agattcacac atgaaaaaag agattgtcgt ccgtccatta caaatttatt aaggagaatt
     2641 gcctacggta attcattccg atagaggtag ccagcatcgg tcttttattt atcaagagct
     2701 gttgaacgac caccacatca cacattcagt gagccaaact gggactcctg ttgacaatgc
     2761 agtaattgaa gcgtttcatc gatcaatcaa atgagaatta atcgactcca acaaatataa
     2821 aagtaaaata gagatgaagg tgttaatcca agattattta gaagactact acccaaacaa
     2881 aagaatccat acaaaattta tgatgactcc ttgacaattt gaagcgaaga aaataaggga
     2941 tctataatat ctagtgttgg tgacgccttc tgttgccaat actttttctt ttttctaata
     3001 aacgcaaaca gacaccttaa aatcctttag aataaagtcg acaaaaacca ttcaaagtag
     3061 tgtttcaaga tgtcctattc agagtttacc aaagagttac tagatattct agacctaaat
     3121 cttacctttc atgaggatgc ctttcgcaaa gagcgcatca atgacgaaac atgttttgct
     3181 tttgatggca cattgaccta tcaaccggag gaatgctttc atcgccacta tcaaaatgaa
     3241 caaacgatca ttaaataggg ctggaaaaaa gtatccatct tattgaatga tgtcagtaac
     3301 tacaagacca ttctgcggat taacaaacag cgcttcaaat gtaaacactg tggaaagact
     3361 tttttagcag aagattcagt tagtgatcgt cgctgctcca ttgctcgcag agtcaaacaa
     3421 gccattcttg agctattaag cgaacctctt tctatgtctt tgatcgcacg tatgaagcac
     3481 atttctccga ccactgtcat tcgaata
//
LOCUS       DACGLE010000136         3391 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0534
            SAMEA3304449-rid9560453.denovo.146, whole genome shotgun sequence.
ACCESSION   DACGLE010000136 DACGLE010000000
VERSION     DACGLE010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304449
            Sequence Read Archive: ERR985064
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3391)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3391)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702225.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702225.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 20:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,045
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 27 of 67
            Pseudo Genes (incomplete)         :: 25 of 67
            Pseudo Genes (internal stop)      :: 19 of 67
            Pseudo Genes (multiple problems)  :: 4 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3391
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304449-rid9560453.denovo.146"
                     /strain="VREN0534"
                     /db_xref="taxon:1352"
     gene            647..964
                     /locus_tag="IXT20_002737"
     CDS             647..964
                     /locus_tag="IXT20_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HAQ4861138.1"
                     /translation="MLFYSNFILIVAILLLLNIWIFDRSRNSSIGFRTKRSLSSKKNW
                     VYSQTIFYGGIVLISLLSSTLYSLNIIDVSTSNSISIIGIIIAAIITQLFLVFGEKKR
                     SKK"
     gene            complement(1055..1420)
                     /locus_tag="IXT20_002738"
     CDS             complement(1055..1420)
                     /locus_tag="IXT20_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4861139.1"
                     /translation="MIKTCWKNLPLLLSFVPYVHFALLLDFRYHSVSGFITLIFLSLF
                     AGYYFQRNRRIISLFIANIISTVTSYLFCANFTEWRYFYHPLKPTQLILLLAGIYLVP
                     QILGSLWAVALSYKKARHP"
     gene            1628..2110
                     /locus_tag="IXT20_002739"
     CDS             1628..2110
                     /locus_tag="IXT20_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4861140.1"
                     /translation="MRRKAVIDRLYLFFWFFPIVLLPFTWVNAVVQGAEILGLIQGSG
                     FVVSRNFPALIVFYLVISVGLLLPKSLINHYNWQLLYFSCLLVFTSMFSLLPRLIIGP
                     NGLSESGLKDLAYSSMYFLTIKPWFYLVIASFILATFFYFWTEIRIKSERSVSIKNNY
                     "
     gene            2307..3197
                     /gene="pstS"
                     /locus_tag="IXT20_002740"
     CDS             2307..3197
                     /gene="pstS"
                     /locus_tag="IXT20_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS family protein"
                     /protein_id="HAQ4861141.1"
                     /translation="MKKVLFTLGMMGILLAGCGSGSGNTDGSATQNNSSDSNEQVKIV
                     AVGSTALQPLVDAAQESFVQENPNYQISVQGGGSGTGLSQVEAGAVTIGNSDVFAEER
                     DGVDASKLVDHKVAVVGMAPIVNKDTDVKDITKQELIDIFTGKITNWKEVGGKDQKIN
                     VVNRANGSGTRATFEKWGLDGATPVQSQEQDSSGTVRQLVSQTPGAISYLAFSYLDDS
                     TQALSIDGVEPKEENVADNSWGIWSYEHMYTNGKPSPEVQKFLDYMMTEEIQEGPVKE
                     LGYLPITMMEVERDHEGNIK"
BASE COUNT         1172 a          519 c          619 g         1081 t
ORIGIN      
        1 ttattgcttt tgcaaaattt atttacctgt tttctggatc aacgatagtg tacattatgc
       61 tatccttgtt tggattttag gacgtttaaa gaggcacttt tttacataac ttaatccata
      121 gaccttttaa gcatgctgtg agataataat aaagaagagt ttaaagcgca ccccaaacca
      181 cttccccata agtgtattac gctttaaact cttttatatt tgaattcact aagaagcata
      241 ccatatattt gaatttacta agaagcatac catatttttg aaaaaaagtg agaaaaaagg
      301 cttataatta ggatttagag taattaatta gatgtttatt ttataggact gatactatag
      361 attacaaata gtatcgtatt tcgcaatctt aaaattatct tcttactgtg aataagataa
      421 taaaatttgt atttgtcctt tttaggtaac ttattttaat ctatatacct ttctggatca
      481 acgaaagtat actttatata gtcataacgc cgatggtaac tatgcactgt aacgataatg
      541 ctgctaaaat atctttttac gaaaaacatc acaaggaaaa acttaactat ggtattatct
      601 aaacataatt ttttcggtat ttaatattta attaggaggt aatattttgc ttttttatag
      661 taattttatc ttaatagtag cgatattatt acttctgaat atatggattt ttgataggag
      721 tagaaattca agtataggtt ttagaactaa acgaagtttg tcttctaaaa aaaattgggt
      781 atactcacaa actatttttt atggaggaat tgttttaatt agtttacttt cttcaacact
      841 ttattctttg aatattatag atgtttccac ctcaaattca atatctatta ttggaatcat
      901 tattgctgca attattacac aactgttttt agtgtttgga gagaagaaaa gaagtaaaaa
      961 atagaaaaac tagtgatagt tttggtgata taagaaaaac atatatgtat aaaaagaaga
     1021 aagtagcttc tctttgtaca tatatggttt gtttttatgg atgccgagct tttttataag
     1081 ataaggctac cgcccataac gacccaagga tttgtggaac taagtaaata cctgcaagaa
     1141 gcaagatcag ctgggtcggt tttaaaggat gatagaagta acgccattca gtaaaattgg
     1201 cgcaaaataa ataggaagtg acagtagata taatattggc aatgaataaa gatataatac
     1261 gcctgttcct ttgaaaataa tatccagcaa aaagagataa aaatatcaat gtgatgaacc
     1321 cgcttacgga atgataacga aaatcaagca gtaaggcgaa atgcacataa ggaacaaaag
     1381 ataaaagcaa cggaaggttt ttccagcaag tttttatcat ctttcatcac cacctaacaa
     1441 gtgttattat tttgttagtt ttattgtaaa ttgaatcctg caataatcaa ttactttttc
     1501 attatttcta gattaaatga ataaactctc atgccattat ctctaaaaaa aaagtttttc
     1561 cccctaattt ttttagatag aaaattgtat agtatgatga gagaaaaagt agtttggggg
     1621 aacgttaatg agaagaaaag cagtaatcga tcggctttat ttattttttt ggttttttcc
     1681 tatcgttttg ctacctttta catgggtaaa tgctgttgta caaggtgcgg agatattagg
     1741 cctgatccaa ggaagcggat ttgtggtttc taggaatttt ccagctttga ttgttttcta
     1801 tttagtgata agtgtaggtc tcttattacc aaaatctctg atcaatcact acaattggca
     1861 gctgctttac ttttcttgct tattagtatt tacatctatg ttttctttgc tgcctcgctt
     1921 gattatcggc cctaatggtt tgagcgagag tggattgaag gatttagctt attcctccat
     1981 gtatttcttg acgatcaaac catggttcta tttagtaatt gcctcattta ttcttgcaac
     2041 atttttttac ttctggacag aaatcaggat aaaaagtgaa cgatctgtat ctataaaaaa
     2101 taactattga tggtggatca tcagtaaaaa aacatatgag ctgcgtaaaa tgcatgtttc
     2161 tcatatgctt tttttattat tatgtaaaga atgcgaaaaa cgtttacatg atttttacat
     2221 aacttaacta tatttttaca gcaaccatac gagggattta cagtcgactg ataaacttta
     2281 gttgtaaata agaaaggagc aataaaatga aaaaagtatt gtttactcta ggtatgatgg
     2341 gaattctttt agccggatgc ggctcaggat caggaaatac agatggctct gccacacaaa
     2401 ataattcaag tgactcaaat gaacaagtaa aaatcgttgc ggtaggttcg acagcattac
     2461 agccgttagt cgatgcagca caagaaagct ttgtacaaga aaatccaaat tatcaaatct
     2521 ctgtacaagg aggaggaagc ggaactggtt tgagccaagt cgaagctgga gcagttacta
     2581 tcggaaactc tgatgtattt gcagaagaaa gagacggagt tgatgcttca aaattagtgg
     2641 atcataaagt agcagtggtc gggatggcac caatcgtcaa taaagataca gatgtcaaag
     2701 atatcaccaa acaggaattg attgatatct ttacaggaaa aatcactaac tggaaggaag
     2761 ttggcgggaa agatcaaaaa atcaacgtcg tgaaccgtgc aaacggcagc ggtacccgcg
     2821 cgacattcga aaaatgggga ctggatggtg ctacccctgt gcagtcccaa gaacaagatt
     2881 catcaggaac agttcggcag ctggtaagtc aaacaccagg agcaatcagc tatttagctt
     2941 tctcatatct cgatgattcc acacaagcac taagtatcga cggtgtagaa ccaaaagaag
     3001 agaatgtagc agataacagc tggggaatct ggtcatatga acatatgtac acgaacggaa
     3061 aaccttcccc tgaagtccaa aaattcttag actacatgat gacagaagaa atccaagaag
     3121 gacctgtcaa agaattgggc tatttaccaa tcacaatgat ggaagtagaa cgtgatcacg
     3181 aaggaaatat caaataacta gcagataaac atgccacggt agtcgttact attaaggaaa
     3241 aatcaactat gtttccccac atatacattt ttcctttaat tgtgaaaaat agttcctcat
     3301 attttgccgt tttttgagct aataccgagg gtcagctgat tgaacaccgt ttattcgaaa
     3361 aaaagagcta cgacatagaa ctgtcgtaac t
//
LOCUS       DACGLE010000137         3203 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0534
            SAMEA3304449-rid9560453.denovo.147, whole genome shotgun sequence.
ACCESSION   DACGLE010000137 DACGLE010000000
VERSION     DACGLE010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304449
            Sequence Read Archive: ERR985064
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3203)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3203)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702225.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702225.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 20:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,045
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 27 of 67
            Pseudo Genes (incomplete)         :: 25 of 67
            Pseudo Genes (internal stop)      :: 19 of 67
            Pseudo Genes (multiple problems)  :: 4 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3203
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304449-rid9560453.denovo.147"
                     /strain="VREN0534"
                     /db_xref="taxon:1352"
     gene            complement(1646..2164)
                     /locus_tag="IXT20_002741"
     CDS             complement(1646..2164)
                     /locus_tag="IXT20_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013852041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClbS/DfsB family four-helix bundle protein"
                     /protein_id="HAQ4861142.1"
                     /translation="MRTYKNKEELKKEINRTYTKYILEFETIPEELKDKRVEEVDRTP
                     AENLAYQVGWTNLILKWENDERNGLSVKTPSDQFKWNQLGELYKWFTNTYAHLPLKEL
                     EEILNRNIDDINMMIDSMSDEDLFTAHKRKWADEATKTAVWEVYKFIHVNTVAPFGTF
                     RTKIRKWKRLAL"
BASE COUNT         1079 a          567 c          453 g         1104 t
ORIGIN      
        1 ataaagaaaa aattcaagca acaattcaaa aaaatcttaa ctgctgagat tgatgtagca
       61 atatctcaat catcaacaaa gtacgatcct gcctttcaaa agcttattga taaatgttta
      121 ttcattataa aaataaaaga aaaatttatg tcagcttttt tcatcaaagt ggtaatgata
      181 attcgtctat taattgtatt ttttaggaaa tatggtataa ttgttttaac gaaaaggtaa
      241 ttcatgatac accaaaaacc actagctggc gggctagtgg ttttctttta cgttctattc
      301 agattaacgg gtaggcttac tgtctttttt ggcaataatt ttgcaatcaa gccatttaca
      361 gatgtaatgc gcggatactt ctgctaagat tgcaaaaaac atatcgaaaa ggtaattcat
      421 aatacaacct cctccccttg ccataatagg ccggggtagc agacagcata aatattatac
      481 cataactgaa aatttcattt tagtacattt caatatattg atgaaccagc acaataaaac
      541 atgccatgac taacagaaaa aaacttatta aaccagatat aacttgtaaa catccaacta
      601 tatagaaaac gtaaattttc tatatagttg gattaagata acgactattt attttatcat
      661 atttttacta taccctatac ttttacttgg tatatttatg aataatcgca aatttacccc
      721 tatactacta gtttgtaagt tagattaata aaaatactaa agttatttgt tcttttatta
      781 aatacaccta caacttataa attgatggta tttagataac acgaaagaat aaaaataaca
      841 gctgatagta aagtttgttg acgattgcta ctcaatcgaa agtgagttag tgcgtatgaa
      901 cttgcttctg aaaacttatg aaatgaaaga ccttttacat cttgcagaaa tcgttactgg
      961 gattaaacta agcaataaaa actaatgttc ccctcattct ttgaacttct actatttgat
     1021 ttatttattc tgataaatga cgatttcacc ttcaggatct tcaaatcccg tatagaattt
     1081 atttaagatc tcttcagtat cagtaatagg ataggcgata ggccacatac cctttgatgg
     1141 ttagaataaa ctgctcaaaa tctgtatcgt atgttggctt tagcatatta acaaaggcta
     1201 tctccaaatt ataaaccata agcgttgaat cgttcttaac aatttcaatt cttcctaacc
     1261 caaagtcaga agatataatt accggacttg ccttaccctt catgtgggtt gttgagattc
     1321 catatttaaa gggaattgtc ggtactatcg gcggtctgaa aaacatccca aaaataaaaa
     1381 aatctttccc ctgctagtct cctaagtccc tttgtagcgg atatggcacc ataatctcat
     1441 tttatcgaag tgagtcatgg tttatctgtt ggccaaccaa aattgataca atctaacgat
     1501 gcaccctcta cggcattcta ggggagtgca cttgcattca cccaataaaa accggcagtt
     1561 agcaaagaac ttatgtagtc gtatgttctt actgttaaca tacaagaggt ctcctttcca
     1621 caattgctta gcagtaaacg ggaatttaca aagctaatct cttccatttt cttatctttg
     1681 ttctaaaagt cccaaatgga gcaactgtat ttacatgaat aaatttatat acttcccata
     1741 cagccgtttt ggttgcctcg tctgcccatt ttcttttgtg tgcagtgaat agatcttcat
     1801 cactcattga atcaatcatc atgttaatat catcaatatt acgattgaga atttcctcta
     1861 attctttcaa tggtagatgt gcataggtat ttgtaaacca cttatataac tctcctaact
     1921 gattccattt aaattgatca gaaggcgttt taacagataa tccatttctt tcatcatttt
     1981 cccattttag aattaaattc gtccaaccaa cttgatatgc taaattttca gcaggtgttc
     2041 tatccacttc ctcaactctt ttatccttca attcttcagg aatagtttca aattctaaaa
     2101 tatatttagt ataagtccta tttatttctt tttttaattc ttctttattt ttatatgttc
     2161 tcattatatt acctccataa attcctaatt gtctccttta attcatctat aatttcatcc
     2221 acgatatttt aatccgttct acaatgcata tctgatatgc attgactgtc taactcatat
     2281 gtgaatgatt tataaaatat cccaacgtct catctaaaga aatgttgatc gtcagcaagg
     2341 agctaaaaaa tacgaatata tttcttttgt agtaaaatcg gactggtttg catatcattt
     2401 tcttcgtaaa tatcaaaatc atgttaacta ctatcaatta ctaataaaaa ctaccacttt
     2461 aagcgttaag aactgttaag tttagttaag tgaggatagt tcacaaaatc cattgttaaa
     2521 cttcacgaaa gtggtaaaac tcaaaatgct ttatatagga ttacagcatc gctctttctt
     2581 cgattgcgcg ttgggtaaaa aatattatga agtaaaaatt gacgatgaca cgttccttac
     2641 ggctcatcag atgaaacagc tacaaaaacg aaatgatcaa ctcgaagaaa atttaattct
     2701 aaaaaagcga ttgccatatt cacgcctcgc tcgacgaact attaaaggtc aagatttacc
     2761 tgaagatcat cctttttagg aaattgaagc tttttatgtt gcacaatgca tttataatac
     2821 tacaatgatg ttttcaccag atattcttct agggtgcggt gtaatgaaac agatacactt
     2881 gcaaaagaaa attcaccgcg aatttactaa aattatgaat aattatgtta atcttccgaa
     2941 atttttttcc aaaattaagg gatcaagctg gtgttataga ttgtttaagg ttggcaaaaa
     3001 aataacttct ttttaaacaa ttatagaaaa ccgatactct gttaagttgg ttgacacggt
     3061 gtcgattttt atgactttct tattctgaaa cttcactaac ggacagtacc taaaaagtct
     3121 atagtatgga cgtttacgag tactatttta tcaaaaatgc tgctcatttc aacttatatg
     3181 ttttagtgaa ctacccccac tta
//
LOCUS       DACGLE010000138         3132 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0534
            SAMEA3304449-rid9560453.denovo.148, whole genome shotgun sequence.
ACCESSION   DACGLE010000138 DACGLE010000000
VERSION     DACGLE010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304449
            Sequence Read Archive: ERR985064
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3132)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3132)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702225.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702225.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 20:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,045
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 27 of 67
            Pseudo Genes (incomplete)         :: 25 of 67
            Pseudo Genes (internal stop)      :: 19 of 67
            Pseudo Genes (multiple problems)  :: 4 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3132
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304449-rid9560453.denovo.148"
                     /strain="VREN0534"
                     /db_xref="taxon:1352"
     gene            167..562
                     /locus_tag="IXT20_002742"
     CDS             167..562
                     /locus_tag="IXT20_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4861143.1"
                     /translation="MPKKRRKTIVIILTILTIGIGGKFYMDKREMQRQKDLLAVEKQS
                     VKVLKNTFADIKEVKIEKSARNEMTGSYRIVILMTNKQDQSIYFSYSFWKERNEIGSY
                     GIVDEKKQKEGNTLNKVKVTYSNGNEESI"
     gene            562..813
                     /locus_tag="IXT20_002743"
     CDS             562..813
                     /locus_tag="IXT20_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4861144.1"
                     /translation="MITDKQYSELSDAVYWLDPKHRDYVPEMQENLSFKINGTMYKIL
                     KIKNSFDGMQAMAVAPIVHSKLEKNFKNKKIPANFRVLK"
     gene            856..1251
                     /locus_tag="IXT20_002744"
     CDS             856..1251
                     /locus_tag="IXT20_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4861145.1"
                     /translation="MSRKIRKLSLAILLIVILGIGGKVYMDKREAQRQKDLLAVEKQS
                     VKVLKNTFADIREVKVEEFKKNPVTGSYGALVTMTNNEGKSVYFDYSFWKERNELGGF
                     GIENRAVQKTGVTIKRVKVIFSNGQEELV"
     gene            1248..2504
                     /locus_tag="IXT20_002745"
     CDS             1248..2504
                     /locus_tag="IXT20_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipase"
                     /protein_id="HAQ4861146.1"
                     /translation="MTINDKHYNDISEKVYWLDPKYPRYNEGYKKNSVKEFAGMEFQI
                     LQIKDSLDGMQAMSVAPIKNGKVDLSQVVIAFAGTNLSDWKDLETDARSIMNLYGNRP
                     GASFSEKATTNKILSVSGLKTAGQINSAIAFANEVKKEYGEAAISTTGHSLGGFLALY
                     VAAEKQWKNVGFNGPDPYDLLSPEAKKWVEENPGMLTNYRNHADFIGNFGGNGTGAEV
                     RISMETAFLNLLASHSLTSWKFDENGKLIIPENENNVKARKQQKENQATQLFSYQLQE
                     LNNIRKKLRLSGGRLTVNEQIYLDDTQARLVVEKLSAKMKSAMEEVIRINQRGIFELE
                     KKWQEGLRQARVVAPSLTEQEIIEALASVGATKENLVDSAKEVYLLRINKARRMGEAF
                     DTLAKEIQDKINEIVARDRELAQQFN"
     gene            2516..2839
                     /locus_tag="IXT20_002746"
     CDS             2516..2839
                     /locus_tag="IXT20_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4861147.1"
                     /translation="MKLEELERDYRSERRQLEAQEDELIHFKNQGLRLMEQEEQRLHH
                     VLKNLALDNEPLFQARRSYSNYQEHYQQALQVERRKLYQQQDELERSYRQKIRQITME
                     KSSYE"
     gene            2832..3098
                     /locus_tag="IXT20_002747"
     CDS             2832..3098
                     /locus_tag="IXT20_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4861148.1"
                     /translation="MNEFLMQLLKELEKNLHRSNVRSVGMGISQQIWAEKRLTDQNYQ
                     MTAEEAAQEFSNRHVHLKGSVKGQFAEKLTELFQRQEKKLKQFR"
BASE COUNT         1219 a          480 c          659 g          774 t
ORIGIN      
        1 ttttgttcac tactatcaaa attactttaa aatgaaaacg tgataaaaaa gaaagtagaa
       61 aagtagataa gtaactctaa aaaatccaaa aaaatccagg ctcgattgcg tacttatctt
      121 aaaggagaaa caatccatca tatataaccc gaaaggagac ttctatttgc ctaagaaaag
      181 acgaaagacc atagtcatca ttttgaccat tcttactata ggaatcggag ggaaatttta
      241 catggacaaa agagaaatgc aaagacagaa agatttactc gcagtagaaa aacagtccgt
      301 caaagttctg aaaaatacct ttgcggatat caaggaagtg aagattgaaa aatctgctcg
      361 aaatgaaatg acgggttcat accggatagt tattctaatg actaataaac aagatcaatc
      421 gatttatttt tcttatagtt tttggaaaga aagaaatgag ataggaagtt acggaatagt
      481 tgatgaaaaa aagcaaaaag aaggaaatac gctcaataaa gttaaagtaa cttactctaa
      541 tggaaatgag gaaagtattt aatgattact gacaaacaat atagtgaatt aagtgatgct
      601 gtttactggc tagatcctaa acatagagac tatgtacctg aaatgcaaga aaatttaagt
      661 tttaaaatta acggcactat gtataaaatc ctaaaaatca aaaactcctt cgacggcatg
      721 caggcgatgg ctgtagcgcc aatcgtccat agcaaattgg agaaaaattt caaaaataaa
      781 aaaattcctg caaactttcg cgtacttaaa taatgaaggt gtcaatactt acataaaaag
      841 aaaggaggca cgttcttgtc aaggaaaata agaaaactca gcttagccat tttattaatc
      901 gtcatattag ggatcggagg gaaagtctat atggacaaaa gagaagcaca aaggcagaaa
      961 gatttactcg cagtagaaaa acagtcagtc aaagttctga aaaatacctt tgcggatatc
     1021 agggaagtga aggtagaaga atttaaaaaa aaccctgtta ccggaagtta tggtgcactt
     1081 gtgacaatga caaataatga agggaaatct gtttattttg attatagttt ttggaaggag
     1141 agaaatgaac ttggtggttt tggcattgag aacagagcgg tacaaaagac aggagtcact
     1201 ataaaaagag taaaagtaat tttttctaac ggacaggagg agttagtatg acaataaacg
     1261 ataagcatta caatgatatt agtgagaagg tttattggct agatcccaaa tatccccgct
     1321 acaatgaggg atacaaaaag aactctgtta aagaatttgc tggtatggaa tttcaaatcc
     1381 tacaaatcaa agactcattg gacggcatgc aggcgatgtc tgtagcgcca atcaagaacg
     1441 gtaaagtgga tctttctcaa gtagtgattg cttttgcagg aacgaactta agtgattgga
     1501 aagatttgga aacggatgca agaagtatca tgaatctcta cgggaataga ccaggagcaa
     1561 gtttttctga aaaagcaaca acaaacaaga ttttatccgt tagtggattg aaaacagcgg
     1621 ggcaaatcaa ttcagcgatt gcatttgcta atgaagtaaa aaaggaatat ggagaagctg
     1681 ctatttctac taccggccat tcattaggtg gattcttagc attatatgta gcagcagaga
     1741 aacaatggaa aaatgtcggc ttcaacgggc cggacccgta tgatctctta tcaccagaag
     1801 caaaaaaatg ggtggaagaa aatcccggta tgctcaccaa ttaccgaaat cacgccgatt
     1861 ttatcggtaa ttttggtggt aatggcacag gtgctgaagt tcggatcagt atggaaacag
     1921 cattcttgaa tctgcttgct tcccacagtt taacctcttg gaagtttgat gaaaatggaa
     1981 agctgattat tcctgaaaat gaaaacaatg ttaaagctcg aaaacaacag aaagaaaacc
     2041 aagcgaccca gttgttttcc tatcagttgc aagaactgaa caacatacgt aaaaaactac
     2101 gattaagtgg cggccggctg acagttaatg aacaaattta cttagatgac acacaagcta
     2161 ggttagtagt ggaaaagtta tcggcaaaaa tgaaaagcgc tatggaagaa gttatccgca
     2221 tcaatcaaag aggaattttt gagctagaga aaaaatggca agaaggcttg cggcaagcac
     2281 gtgttgtagc cccttcgttg actgaacagg aaatcataga agcattagct agtgtgggcg
     2341 ccacaaaaga aaacttagtc gattctgcga aagaagtcta tctgctacga atcaataagg
     2401 caagacgtat gggggaggcc tttgatacat tggcgaagga gatccaagac aaaatcaatg
     2461 aaatcgtggc acgagatcga gaattagcac agcaattcaa ttaaaggaga agtagatgaa
     2521 actagaagaa ttggaaagag attatcgaag tgaacgaaga caattagaag ctcaagaaga
     2581 tgaattgatc cactttaaga atcaaggatt acgattgatg gagcaagagg agcagcgact
     2641 gcatcatgta ttaaagaact tagctttaga taatgagcct ctgtttcaag caagacgaag
     2701 ttattcgaat tatcaggagc attatcaaca agccctacaa gtagaacgac gaaaactcta
     2761 tcagcagcaa gatgaactag aacgttccta tcgtcaaaaa atcagacaaa taactatgga
     2821 gaaatcatct tatgaatgaa tttttaatgc aattactaaa agaattagag aaaaatctac
     2881 atcgatcaaa tgtacgttca gttggtatgg ggatatctca gcaaatttgg gcagaaaaac
     2941 gattgacaga tcaaaactac caaatgactg cagaagaagc tgctcaagaa ttttctaacc
     3001 gacatgtaca cttgaaagga tcagtaaaag ggcaatttgc agaaaaacta actgaattat
     3061 tccaaagaca agaaaagaaa ttaaaacagt ttcgttagta aagttatata gaaaaatttt
     3121 gtgaaaatgg tt
//
LOCUS       DACGLE010000139         3011 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0534
            SAMEA3304449-rid9560453.denovo.149, whole genome shotgun sequence.
ACCESSION   DACGLE010000139 DACGLE010000000
VERSION     DACGLE010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304449
            Sequence Read Archive: ERR985064
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3011)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3011)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702225.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702225.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 20:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,045
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 27 of 67
            Pseudo Genes (incomplete)         :: 25 of 67
            Pseudo Genes (internal stop)      :: 19 of 67
            Pseudo Genes (multiple problems)  :: 4 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3011
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304449-rid9560453.denovo.149"
                     /strain="VREN0534"
                     /db_xref="taxon:1352"
     gene            177..359
                     /locus_tag="IXT20_002748"
     CDS             177..359
                     /locus_tag="IXT20_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HAQ4861149.1"
                     /translation="MINLFLLVTASTLFFTGISLIISYLIYKKAENHTSKAIGLAVSS
                     FLTLLTLFVTLSFSFV"
     gene            462..746
                     /locus_tag="IXT20_002749"
     CDS             462..746
                     /locus_tag="IXT20_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4861150.1"
                     /translation="MLTATLQQPTHVAIAESYTINAGIVKQLIKDREFKKFNSRQAYN
                     RQLKKAILKHKDSFIGNLESVDVKTVSTAFSVVEVTIDGRLISLFAFGIN"
     gene            762..998
                     /locus_tag="IXT20_002750"
     CDS             762..998
                     /locus_tag="IXT20_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4861151.1"
                     /translation="MEGKTFAEIWTEFIEVPTRIDPITSEKVLDADFYNLKAGTSLEC
                     VWMYLEEHFKGVTLGHVLYHNGAEWDAIGNIHFS"
     gene            1020..1274
                     /locus_tag="IXT20_002751"
     CDS             1020..1274
                     /locus_tag="IXT20_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4861152.1"
                     /translation="MNYTDEEQIALEYVFTNEEGEEIEKVLIISSKDLEKALAYEPYE
                     STVTVIEFLDTYTSNDTWEIMNICNANNIQFAEKTNKSFY"
     gene            1356..1541
                     /locus_tag="IXT20_002752"
     CDS             1356..1541
                     /locus_tag="IXT20_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HAQ4861153.1"
                     /translation="MPKTAREIVKILKSNGFKKTGQTGSHAKFYNSKTKRTVVVPIHN
                     GDVPLGTEANIWKQAGL"
     gene            1608..2003
                     /locus_tag="IXT20_002753"
     CDS             1608..2003
                     /locus_tag="IXT20_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin HicB"
                     /protein_id="HAQ4861154.1"
                     /translation="MKTKNIFYPAIFEKEGEAYNIVFPDLPEISTFGNSLEEAYLNAK
                     DALGLALYSVPDAEFPKPSAVEELTLKANQVVVIIQLNIKLFRRSLNTKTIRKNVSVP
                     EWLVLLGKEKNINFSQLLQKALEEELLEK"
     gene            1993..2370
                     /locus_tag="IXT20_002754"
     CDS             1993..2370
                     /locus_tag="IXT20_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4861155.1"
                     /translation="MKSKKETEPMKATHLNYFSRTLINNLLKDTTGISVSTKHFANDL
                     IIFEDNNLRIEMFIEQATPNSIPEFCYTHTEKMKHAKSVKDVEALSESLTNALNLVKK
                     AKLLFEFQTDYHNELLAQSEILD"
     gene            2371..2766
                     /locus_tag="IXT20_002755"
     CDS             2371..2766
                     /locus_tag="IXT20_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4861156.1"
                     /translation="MKQEKRKMKQTQTETVNDTLADQLAGYYKLFEMGKAQRLIHYLW
                     KYKILFLFWESTLTFSVLFYKSDFYNQHLVLGSFLLIYTILMLNLYTYITLEEHLVIS
                     DAGIERNIKRVSFKITLNKPTDKKLSEAF"
BASE COUNT         1059 a          507 c          523 g          922 t
ORIGIN      
        1 tttatgttta tgtatatgta tatgaatata aacgtattag gtacgaacac ttttaaattc
       61 ttttctgggc tttgttttta gggtagggct aaaaaaaaca tcaccatctg atccaaaaca
      121 gctcagaaac gcatttaaac gtatctgata ccaatattac agaaggagtg tatcgtatga
      181 ttaacctatt tcttttagtg acagctagta cattattctt tactggaata tcactgataa
      241 tttcctattt gatctataag aaagccgaaa atcacacaag caaagcaatc ggactagccg
      301 tttcatcctt tttaactttg ctgactttat ttgttacttt atcgttttct tttgtttaga
      361 acagctgatc cattagcacc tttttctctt tacattatac acactgttgt gtataatgta
      421 tttgtaaaca agctaattaa cttttaggaa ggagtgacaa gatgctaaca gctactttac
      481 agcaacctac tcacgttgct attgcagaaa gttatacaat taatgcaggg atcgttaaac
      541 agctgatcaa ggatcgagaa ttcaaaaagt ttaacagtcg gcaagcgtat aacagacagc
      601 taaagaaagc tattctaaag cacaaagatt ccttcatcgg gaacttggaa agtgtagatg
      661 ttaaaactgt ttcaacggca ttttcagttg ttgaagtgac tattgacggt cgcttgattt
      721 ctctatttgc ttttggaatt aattgatagg gagtgtgaac tttggaaggt aagacttttg
      781 cagaaatttg gactgaattt attgaggtac caactcgcat tgatccgata acttctgaaa
      841 aggtattgga tgcagacttc tacaatctca aagcaggtac tagccttgaa tgcgtttgga
      901 tgtatttaga agagcatttc aaaggcgtaa cgctcggtca cgtcttatac cacaatggtg
      961 ccgagtggga tgcaattgga aatatccatt tttcttaata taagaaagga gaacacaata
     1021 tgaattatac cgatgaagaa caaatcgctt tggaatacgt tttcacaaat gaagaagggg
     1081 aggaaattga aaaagtcttg atcatttcat caaaagactt ggagaaggct ttggcttatg
     1141 aaccatacga atcaaccgta actgtcattg aattcttaga tacctatact tctaatgata
     1201 cttgggaaat tatgaatatt tgcaacgcta ataacattca atttgctgaa aaaaccaata
     1261 aatcatttta ttagttttgc aataaaacat cctctttttc tattgattcc atacacaaca
     1321 atgtgtataa tagattttgt aaagggaggg tagctatgcc taagacagca agagaaattg
     1381 taaaaattct caaatctaac ggcttcaaaa agactggaca aactggcagt catgcaaaat
     1441 tttataattc caagactaaa agaacggtcg ttgttccgat ccacaatggt gatgtaccac
     1501 taggaacaga agctaatatc tggaagcaag cagggcttta acagccttgc cttcctattt
     1561 ggtattaggc atactatcct ctcttacact agaaaaggag cgaggaaatg aagacaaaaa
     1621 atatttttta tccggcaatc ttcgagaaag aaggagaagc ttataacatc gttttccctg
     1681 acttaccgga aatatctacc tttggaaata gtcttgaaga agcatattta aacgctaagg
     1741 atgctttagg attagcgcta tatagtgtac cagatgcaga atttcctaaa ccatcagcag
     1801 tggaagaatt aactttgaaa gccaaccaag tggtagtaat catccaacta aacattaaac
     1861 ttttccgaag aagcttaaat acaaaaacaa ttagaaaaaa tgttagtgtt ccggaatggt
     1921 tagttctttt agggaaggag aaaaacataa acttctctca actacttcaa aaagcactag
     1981 aagaagagtt gcttgaaaag taaaaaggag accgaaccta tgaaagctac acacttaaac
     2041 tatttctctc gaacgttaat caataatcta ttaaaagata caactggcat tagcgttagt
     2101 accaaacact ttgccaatga tctaattatt tttgaggata ataacttgcg aattgagatg
     2161 tttattgaac aggcaacccc taactcaata cctgaattct gctatacgca cactgaaaaa
     2221 atgaaacatg caaaatcagt taaagatgtt gaagcattaa gtgaatcact aacgaacgct
     2281 ttaaacctcg ttaagaaggc aaaattgtta tttgaatttc aaaccgacta ccacaatgag
     2341 ttgcttgctc aatctgaaat tttagattaa ttgaaacagg agaagagaaa gatgaaacaa
     2401 acacaaacag aaaccgttaa cgatacgctt gctgatcaat tagcaggtta ttataaatta
     2461 tttgagatgg gtaaagcaca gcgcttgatc cactatcttt ggaaatacaa aattttgttt
     2521 cttttttggg aatcaacact aacattctca gttctttttt ataagtcaga tttttataat
     2581 caacacttag ttttagggag ttttttgctt atctacacca ttttgatgct taatttatat
     2641 acctacatca cacttgaaga acacttagta attagcgatg cagggatcga gagaaacatc
     2701 aaaagggtat ccttcaagat cacgttaaac aagcctaccg ataaaaagct ctcagaagcg
     2761 ttctgagagc ttttgacatt ttcttgtata attttatcct aacacattac aaagcaccta
     2821 attcgagctt atatcaagca aatttgctat ttattactca atacatatct aaaaattact
     2881 tgatggaaaa ttagatacat ggtaagcatg aaaagaaaat caatttgagc agatgcagta
     2941 caatacatag caatttaaaa gctctcagaa ggcttctgag agcttttaat atattctttg
     3001 ataatttgtc t
//
LOCUS       DACGLE010000140         2947 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0534
            SAMEA3304449-rid9560453.denovo.151, whole genome shotgun sequence.
ACCESSION   DACGLE010000140 DACGLE010000000
VERSION     DACGLE010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304449
            Sequence Read Archive: ERR985064
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2947)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2947)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702225.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702225.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 20:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,045
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 27 of 67
            Pseudo Genes (incomplete)         :: 25 of 67
            Pseudo Genes (internal stop)      :: 19 of 67
            Pseudo Genes (multiple problems)  :: 4 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2947
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304449-rid9560453.denovo.151"
                     /strain="VREN0534"
                     /db_xref="taxon:1352"
     gene            complement(449..865)
                     /locus_tag="IXT20_002756"
     CDS             complement(449..865)
                     /locus_tag="IXT20_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4861157.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIERNPDINNYSEAIRFLCMSY
                     EPDTNTTTTSNQAKLNAMGKEISIMSEIIAEFADLYLKDTGILIGTNSKVYQETKEKI
                     EKQLNPSKRNQPKNRQKETNQTFTSKNFDPTNFLKR"
     gene            complement(867..1148)
                     /locus_tag="IXT20_002757"
     CDS             complement(867..1148)
                     /locus_tag="IXT20_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4861158.1"
                     /translation="MDILVRKIDSKVIAKIDKRCQELTNKTGKKWSRNRYLKVLIEND
                     FDHALMHYKKDQFDLLLEKFIAIQEKNTETLNEYVRTNNQLIETLIGGR"
     gene            1948..2922
                     /locus_tag="IXT20_002758"
     CDS             1948..2922
                     /locus_tag="IXT20_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HAQ4861159.1"
                     /translation="MSKRGTIINEWEKLNRISNRRLNAQTMCTPLKMQWSIDRITIVG
                     TLRENIYYHTPHDVLILDFEQLMRLNEGNGYLKAVGNNGWQLLDQHEENIAYIEILKF
                     QEGKGRIDFNPNKISQFLAGSMKNFIHDLFLEPHFSRADIACDIIDVPDDFITQYRVV
                     DPVSFKPIYGRSGKLETAYWGSRASERQIRMYNKKLEQERKRKVVPKEIDTWWRLELQ
                     LRRGKATDWHAMVHESLDSFASPHYLPEDVKVTDRMMITALITEHSYWGQIHRNSKYK
                     YRELLKQESQNDELTNHLRETFSESADDLKRELDTWLQGLDVTEVGAE"
BASE COUNT          913 a          438 c          603 g          993 t
ORIGIN      
        1 aaaaaaactt gctgatgaat tgggtgtatc gaaaaatacg gtgcgcaatc aactggtcaa
       61 ttcgggattt gaccttgctc agtataaaga aaaaaatgca gtggtcaatt gaccgaaatt
      121 tatgtctttg ggttttgggg ttttaaaacg aaaaaaaggg ggcgattttc gccccctttg
      181 ctctatgtga aaaatttgtt atacttattt tatccttaaa acataccaat ctttttctta
      241 tttagctgct cgtacgagca gcttttttta cgattatgaa ttcaaatcat gtatttataa
      301 tttttttgtt tttatctttt tttctgttat ttcttctgaa attcgttttt ttcattcaat
      361 tggttactat cttattgtaa atgttgctat taagatggtg atcctaccca aaaaataaac
      421 caggaattta aatctcctgg tttatttttt atctttttag aaaatttgtt gggtcgaaat
      481 ttttagaagt aaatgtttga ttggtttctt tttgacgatt ttttggttga tttcgtttgg
      541 agggattaag ttgtttttca attttttctt tcgtttcttg atacacttta gaatttgtgc
      601 caataagaat acctgtatct tttagatata aatcagcaaa ttctgcaatg atttcggaca
      661 tgatggaaat ttcttttccc atagcattta gtttggcttg gttgctggta gtcgtagtat
      721 tggtatcagg ttcataactc atacataaaa aacgaatggc ttccgagtaa ttgttgatat
      781 ctggatttct ttcgataatg gaatctaaaa cgtccacgtg actgggataa agggtaacgt
      841 gtttttgaac agaagttttt ctcatgttat cttcctccga taagtgtttc aatgagttgg
      901 ttgtttgtac gaacatattc attcagcgtt tcagtatttt tttcttgaat agcgataaat
      961 ttttcgagta ataaatcgaa ttgatctttc ttgtaatgca taagtgcgtg gtcaaaatcg
     1021 ttttcgatta ggacttttaa gtagcgattt cgactccatt ttttgccagt tttatttgtg
     1081 agttcttggc aacgtttgtc tatttttgca ataacctttg aatcgatctt ccgaactaaa
     1141 atgtccatag aaaaactcct ttcgcatttg tgtataattt atgtatatca tacgaatata
     1201 gtatttgtat agctgttttt tgctaaaata ctatacataa gctagccacg atggcgaaaa
     1261 agtgatatca aatttttttc tgagaaacgt gataggcaca gcctatctac ccactttttt
     1321 tagaaagtgg gtacacgttt ccttatgctc tttataaaat ggtgatgtgc gacttttttc
     1381 tgttcgtaaa aataaaaaaa gtttacgaaa atgaaactaa actgaacgat tatgacgggc
     1441 atgtaatcaa gatgaaatga ttttataagc gcgtattttg cgttctaaga cgttttagcg
     1501 ttcatttcgt ttagttatcg gcacaatcgt taaaacacgt aagagaagcg agaatggagc
     1561 ctgtaaggcg actggtagcg aaacggcgtg ttagattgtg caagccgaga actgaatgaa
     1621 atgaagagcg gaagcgtgaa ataatttcgg caggaggagg ctcaaggggt tcggggaatg
     1681 aaattccctg aggtcttttt atttttttaa gaggagtgct ataatcaaag aaaacgagaa
     1741 aaaagagcaa aataaaagcc gaacggtgtt ggaagcaccc ttcggctaat tacggaaact
     1801 actcacctag ttccgtaact caaattcatt atacgaatat ttttaggttt cttcaagcta
     1861 gagatttttg gatatggatt tttagagtta cgtgaactct ggaaattctt tccaaaaatc
     1921 tcttttttct ttgaggagga acaaaatttg agtaaacgtg gcacgatcat aaatgagtgg
     1981 gaaaaactta accgcatatc taacaggcgg ttaaatgcac aaactatgtg caccccctta
     2041 aaaatgcaat ggtcaattga ccgaattacc attgttggta cattaaggga aaatatttat
     2101 tatcacactc cccatgatgt gttgatttta gattttgaac agcttatgcg tctaaacgaa
     2161 ggtaacggat atttaaaagc ggttggaaat aatggctggc aattactaga ccaacatgag
     2221 gaaaatatag cttatattga gattcttaag tttcaagagg gaaaagggcg gattgacttt
     2281 aaccccaata aaattagcca atttctggcg ggctcgatga agaactttat ccatgattta
     2341 tttttagaac cacatttctc acgtgcagat attgcttgtg acattattga cgtgccagat
     2401 gatttcatta cccaatatcg ggtggttgat cctgtttctt tcaaaccaat ttacggtcgg
     2461 tctgggaaac tggaaacagc atattgggga agtcgtgcta gtgaacgaca gattcggatg
     2521 tataacaaaa aattggaaca agaaagaaaa cggaaagttg ttccgaaaga aatcgatact
     2581 tggtggcgac tagaattgca attgcgaagg gggaaagcaa ctgattggca tgcaatggtt
     2641 catgaaagtt tagatagctt tgcaagtcct cactatttgc cagaagatgt gaaagtaact
     2701 gacagaatga tgattactgc tcttataacg gaacatagtt actgggggca aattcataga
     2761 aattcaaaat ataaatatcg tgaattgtta aagcaagaaa gtcaaaatga tgagttgacg
     2821 aatcatttgc gagaaacatt ttcggagtcg gcagatgatt tgaaacgaga attagataca
     2881 tggcttcaag gacttgatgt gacggaagta ggtgctgaat gagtgattcg atagaaaaaa
     2941 tgaccct
//
LOCUS       DACGLE010000141         2947 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0534
            SAMEA3304449-rid9560453.denovo.152, whole genome shotgun sequence.
ACCESSION   DACGLE010000141 DACGLE010000000
VERSION     DACGLE010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304449
            Sequence Read Archive: ERR985064
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2947)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2947)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702225.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702225.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 20:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,045
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 27 of 67
            Pseudo Genes (incomplete)         :: 25 of 67
            Pseudo Genes (internal stop)      :: 19 of 67
            Pseudo Genes (multiple problems)  :: 4 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2947
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304449-rid9560453.denovo.152"
                     /strain="VREN0534"
                     /db_xref="taxon:1352"
     gene            172..1476
                     /locus_tag="IXT20_002759"
     CDS             172..1476
                     /locus_tag="IXT20_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAQ4861160.1"
                     /translation="MISITHTGNLFLDTCLSIMYFFLVSYPILGGFVWFIGVWCYVFL
                     YKHKQKEWVDVPLSVEPFITIMVPAHNEEIVIEDTIEYLMTKLNYHNYEVLVTDDGST
                     DQTPEILARLMKKYANLRVVRIEKNKGKAHAFNIGLAFAKGKLILSNDADTVPEPDAL
                     IRYVNYFIRPGARHIAAVTANMDVQNRTKLIAKSQTVEFSSIVGIIKRTQSAVFGGLY
                     AYSGANTMYRKEALIDVGGFRQDRATEDISIAWDHQLNDWLSVFAPEIIFFMEVPVTL
                     KMLYRHRKRWAKGGTEVWLTNFKKVMLHPFKHIGRTIIFIDQTLSIVWSIFFCISVVL
                     FAGLIGHYVYQGNYEQIYITFTFSFVFICFEMVAGFFQLLASLIVDDRRRKLKYLLFA
                     PLYMLLFWIVNAITIVTTFIPAVKTILGYGSGTWKSPERTKK"
     gene            1572..2441
                     /locus_tag="IXT20_002760"
     CDS             1572..2441
                     /locus_tag="IXT20_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015370988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAQ4861161.1"
                     /translation="MGKVMKKRMKPLIWPIAVSSIFLLGQTAFAETSEPAQTEPSTTI
                     SSSGAENGASETEMTSNVPTDNSGETISSSEDTSINSSTSSDQIDHLQDLENKRKELE
                     EALEAAKNKLKEDQAAIDQDLADLKNKVGNDRAQLEKKLAEAKAKAEAVQTDLDGKIQ
                     QAKEFNQQLNQVAQTASGKLSQTQADITAASQAIHGKISEAQTVANDFEAKKADLAAS
                     FQRIKDILHSAGEKSVTSGSTTETVQQTTEPSKSTSQTLPKTNEKTASVSYSLAGAGV
                     LVAAAYGIIRKKK"
     gene            complement(2553..2873)
                     /locus_tag="IXT20_002761"
     CDS             complement(2553..2873)
                     /locus_tag="IXT20_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HAQ4861162.1"
                     /translation="MITTTTNSVEGKAVVAYKGIVFGEVITGIHAFKDLEAGLRNIFG
                     GRSKSYENELMGAREEALAEMAERAKALGADAIIGVKMDYEVLGSDNGMLMVTCSGTA
                     VSTR"
BASE COUNT          974 a          557 c          596 g          820 t
ORIGIN      
        1 tgaagatgaa aaattgaaaa aaagacaaga attacttgaa gaagtgtata cggaacgttt
       61 tggaacaaaa gaagagagag aaacggtccg tttttattct gtctccgaag agaaaaatct
      121 agatacgaca ttcattgcag atttgtataa agaaaatggg gtggaactga aatgatctca
      181 atcacccata caggtaattt attcttagat acttgtttgt ctattatgta tttttttctc
      241 gtttcttatc cgattttagg cggatttgta tggtttattg gtgtctggtg ttatgtgttt
      301 ttatacaaac ataagcaaaa agaatgggta gatgttccct tgagtgtcga accgttcatc
      361 acgatcatgg tgccagccca taatgaagaa attgtgatcg aagatacaat cgagtatttg
      421 atgacgaaat tgaattatca caactatgag gtattagtga cagacgatgg ttcaacggac
      481 caaacaccag aaatattagc tagactgatg aaaaaatacg ctaatcttcg tgtggttcgg
      541 atcgaaaaaa ataaaggaaa agcccacgcg tttaatatcg gacttgcctt tgcaaaaggg
      601 aaattgattt taagcaatga tgcagacacg gtaccggagc cagatgcact gattaggtat
      661 gtgaattatt tcattcgccc tggtgcaaga catattgctg cggtcactgc aaatatggat
      721 gtccaaaatc gtaccaaatt gattgcaaaa tctcaaacgg ttgaattctc aagtatcgtg
      781 ggcatcatta aacgaacaca gtcagctgtt ttcggtggtc tttatgcgta cagtggagca
      841 aatacgatgt atcgaaaaga agccttgatc gatgtgggcg gattcagaca agatcgagcg
      901 acagaagaca tcagtattgc ttgggatcat cagttaaatg actggttatc tgtttttgct
      961 cctgagatta tctttttcat ggaagtacct gtgacgttga agatgctata tcgacacaga
     1021 aaacgttggg caaaaggtgg aacagaagtc tggctgacga atttcaaaaa agtgatgctc
     1081 catcctttta aacatattgg aagaacgatc atcttcattg atcaaacatt gagtattgtc
     1141 tggtcaatat tcttttgtat ttccgtagtt ctttttgctg gattaatcgg gcattatgta
     1201 tatcaaggaa actacgaaca gatttatatt acttttacat tcagttttgt gttcatctgc
     1261 tttgaaatgg tcgcaggatt tttccagttg ctagcttcgc tgattgtaga tgatcgacga
     1321 agaaaattga aataccttct tttcgcacca ctatatatgc ttttgttctg gatagtgaac
     1381 gcgataacga tcgtgacgac tttcattcca gcagtcaaaa cgattttagg ttatggaagc
     1441 gggacatgga aaagcccgga acgtacaaaa aaataaaaga tgcaccgttt tttagacaaa
     1501 cagcctgttg tgtttatggt tcttccaaac atttttgctt ataataagaa attagttaaa
     1561 aagtagtaag gatgggaaaa gtgatgaaaa aaagaatgaa gccgttaatc tggccaattg
     1621 ctgtttccag tattttctta ttgggacaga cagcttttgc ggaaacttct gaacctgctc
     1681 aaacagaacc ttcaacaacc ataagctctt ctggtgctga aaacggtgcc tcggaaacag
     1741 aaatgacttc caatgtaccg acagataatt caggagaaac gatttcatct tcagaagata
     1801 catcaataaa tagttctacc tcttctgacc aaatcgatca tttgcaagac ttggaaaata
     1861 aaagaaaaga actcgaagaa gcattagaag ctgcaaaaaa caaattgaaa gaagaccaag
     1921 cagcaatcga tcaagattta gctgatctta aaaataaagt gggaaatgac cgtgcacaac
     1981 ttgagaaaaa gcttgccgaa gcgaaagcaa aagcggaagc tgtccaaaca gatcttgacg
     2041 ggaagatcca acaagcaaaa gaattcaatc agcaattaaa tcaggttgct caaacagctt
     2101 ctggaaagct aagtcaaact caagcggata tcacagcggc cagtcaagca atccacggaa
     2161 agatcagcga agcacaaaca gttgctaatg attttgaagc aaaaaaagct gacttagcag
     2221 ctagtttcca acgaataaaa gatattttgc attcggcagg agaaaaatca gtgacctctg
     2281 gaagcacgac agaaacagtt cagcaaacga cagagccatc caaatcaaca agtcagactt
     2341 tgccaaaaac aaatgaaaaa acagcttctg tcagttattc cttagcaggg gcgggtgtgt
     2401 tagtcgcagc agcttacgga atcatccgca aaaagaaata aaaaagagag gatgggaagg
     2461 tagagtacct cgaagttaga ctgaaaaatc taattcttga gtgccactac atgtcccatc
     2521 tctctttagt tattaaaaca gatgtcccct acttatctcg tggagacagc tgttccacta
     2581 catgtgacca tcagcatgcc attgtcgctg cctaatactt catagtccat ttttacgcca
     2641 atgatcgcat cagctcctaa tgcttttgca cgttcggcca tttcggcaag tgcttcttct
     2701 cttgcaccca ttagttcgtt ttcatagctt tttgagcggc cgccaaagat atttcgcaac
     2761 cccgcctcca agtctttaaa tgcatggatc ccagtaatga cctcaccaaa aacgatccct
     2821 ttataagcta ctacggcttt tccctctaca ctgttcgttg ttgttgtaat cattcgatca
     2881 ctcctctctt ttaattaaag tataacaaac ttcaagaagc actacacata ttttcccaaa
     2941 attagaa
//
LOCUS       DACGLE010000142         2916 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0534
            SAMEA3304449-rid9560453.denovo.153, whole genome shotgun sequence.
ACCESSION   DACGLE010000142 DACGLE010000000
VERSION     DACGLE010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304449
            Sequence Read Archive: ERR985064
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2916)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2916)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702225.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702225.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 20:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,045
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 27 of 67
            Pseudo Genes (incomplete)         :: 25 of 67
            Pseudo Genes (internal stop)      :: 19 of 67
            Pseudo Genes (multiple problems)  :: 4 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2916
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304449-rid9560453.denovo.153"
                     /strain="VREN0534"
                     /db_xref="taxon:1352"
     gene            complement(<1..1003)
                     /locus_tag="IXT20_002762"
     CDS             complement(<1..1003)
                     /locus_tag="IXT20_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4861163.1"
                     /translation="MDDIKEVFLEVLSRYIQDNLLEYQNNGKKYEFSFDIKNYNDSLH
                     VVFQYNKAGKIGITDVHSFQIFLNEINIEASKSHLNYTTILDTNSLFFSKKLLPYLHS
                     YEWALRKLIYLLAPTYFDNWTKDSIPDEMISAVKGKQKQAIGKYDLENLLQWIDLYDF
                     EEYLFGENYLFISTGNEKNIVRYKEIIVEELLNLFRETDDLSLSDPFSLWSEVFSKYM
                     YVDLEEIRKDMELIREGRNIVSHNKEIKYSLYKDLIKNLKKYVKQLNEAFKKILIGEI
                     ADEKLFDMTNDFEAYIGNHYSNINLPNATKLLTSLSAMTVLNTDSTKKMMETIGPALS
                     "
     gene            complement(1172..2101)
                     /locus_tag="IXT20_002763"
     CDS             complement(1172..2101)
                     /locus_tag="IXT20_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4861164.1"
                     /translation="MRSDSRFFVSNLQDDELIRQIDSLLETITESDKRIFLNYVELTR
                     HIIELDKLFNVFRYNLTNLLKHFTIFTNDLIESTGEKLTEDQYYYQINALTINLISSA
                     KTLTESIEVCMKNFLAEKDFKSFKNKILSKPYDEHFSYRFLLHVRNYSQHGHLPVNIE
                     QQRVYFDLDEILTMPHFDLNEKLKSEIDEIKEDISARFEDFPRISYVYTIAKFNLITT
                     EIYLNYLKEVKPILMEMDKEKNELLLNTKFKLTNSDGKSSDVVFYDFDGENYHCFNRT
                     DNSLSMYASIKKEVKKILREEEQYYKEIKNKNQ"
     gene            complement(2349..2699)
                     /locus_tag="IXT20_002764"
     CDS             complement(2349..2699)
                     /locus_tag="IXT20_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4861165.1"
                     /translation="MIFENESEEIGRVAEENKVDPLETLVINKKEFEQLFLDFFIQYD
                     MIYDFSSSLDLFELEAEIIENSREKNGKIIISVEKFNELFSETFEIDPDYYQPQEVFR
                     EFRNQIIRSSNIEN"
BASE COUNT          960 a          485 c          324 g         1147 t
ORIGIN      
        1 cggataatgc cggaccgatt gtctccatca tttttttagt ggaatcggta tttaatactg
       61 tcatcgctga taaagacgtc aataatttag tagcgttggg taggtttata ttactataat
      121 gattccctat atatgcttcg aaatcatttg tcatatcgaa aagtttttca tctgctattt
      181 caccaattag tattttctta aatgcttcat taagttgttt cacgtatttc tttagattct
      241 ttatcagatc cttatataaa gagtatttaa tctctttatt atgactcaca atattccttc
      301 cttctctgat caattccatg tcttttcgaa tttcttctaa atcaacatac atatacttgc
      361 taaatacttc actccataat gaaaaaggat cagatagact taaatcatca gtttctcgaa
      421 ataaatttaa caattcttct acgattatct ctttgtacct tacaatattt ttctcatttc
      481 cagttgaaat aaataggtaa ttttctccaa ataggtattc ttcaaaatcg tataaatcta
      541 tccactgtaa aagattttct aagtcatact tgccgattgc ttgtttttgt tttcctttta
      601 ctgcagaaat catttcgtct ggaatagaat ctttcgtcca gttgtcgaaa tatgttggag
      661 ccaataaata tatcaatttt cttaaagccc attcataact atgcaaatat ggtagtaatt
      721 tttttgaaaa aaataatgag tttgtatcta aaatagtagt gtaatttaaa tgtgacttgc
      781 tcgcttcaat attaatttca tttagaaaaa tttgaaatga atgtacatcg gttatcccta
      841 ttttaccagc cttattatac tgaaatacta catgtaacga atcattataa tttttaatat
      901 caaaactaaa ttcgtatttt tttccgttat tttgatattc taaaaggttg tcttgtatat
      961 atcgtgataa tacttccaaa aaaacttctt taatatcatc cattttgcct tgtttagggt
     1021 aatataaata ttcaacaact tgcttgctaa tatgtcctcg ccttcttttc tttcaacgat
     1081 ttaaaatttt ccaaataact tgataactac taagtaagcc tcaaacaaca accacaaatg
     1141 aatttttcac taatattgat gattgtgata tttactggtt tttatttttt atttctttat
     1201 aatattgttc ttcttctctt aatatctttt taacctcctt ttttatgcta gcatacatcg
     1261 ataaactatt gtctgttcga ttgaaacaat gataattctc tccatcaaag tcataaaaaa
     1321 ccacatcact tgattttcca tctgaattag ttaacttaaa tttagtattg agtagcaatt
     1381 catttttttc tttatccatt tccatcaaaa ttggtttaac ttcttttaaa tagttcaaat
     1441 atatttcagt tgtaatcaga ttaaactttg caattgtgta cacatatgag atacgtggaa
     1501 aatcctcaaa tcgagcagat atatcttctt taatttcatc tatttctgat tttaattttt
     1561 cattaagatc aaaatgaggc attgttagaa tttcatccaa atcaaagtat actctttgtt
     1621 gttcaatatt taccggtaag tgaccatgct gagaataatt tctaacatgt aataaaaatc
     1681 tgtacgagaa atgttcatca taaggcttac tcagaatttt attcttaaaa gatttaaaat
     1741 ctttttctgc taaaaagttt ttcatacaaa cttcaatgga ttcagtcaaa gttttggctg
     1801 aactaattaa gtttatcgtc aaagcattta tctgataata atactggtct tccgttaatt
     1861 tttcacctgt actttcaatt aaatcattag taaaaattgt gaagtgtttg agcaaattag
     1921 ttaaattata tctaaacaca ttaaaaagtt tatccaactc tataatatga cgtgttaatt
     1981 ctacataatt taaaaatatt cttttatcag attctgttat tgtttctaat aatgaatcaa
     2041 tctgccttat tagctcgtca tcttgtaaat tactgacgaa aaacctacta tcacttctca
     2101 taattatatt ctcctaatat gattttttca aaataatcaa tatgtaacat agaagtatac
     2161 tctattgtta cataccttat ttcttattat atcagtattt ctctgtgaca cataagggtg
     2221 taacataatt attttaaacg aaatgactac aagtagtcat tgaagcaaat ctccataaga
     2281 catttgtaaa atcttttaaa aagaagcgtc gaaagaagga acttttcttc aacgcttctt
     2341 ttattgactc aattttcaat attacttgaa cgaataatct gatttctaaa ctctcgaaaa
     2401 acttcttgtg gttgataata gtctggatct atttcaaatg tttctgagaa taattcattg
     2461 aatttttcta ctgaaataat gattttacca ttcttctctc gactgttttc tattatttct
     2521 gcttctaatt caaataaatc tagtgacgaa ctaaaatcat aaatcatgtc atattgaata
     2581 aaaaaatcaa gaaaaagttg ctcaaactct ttcttattta ttactagtgt ttcaagcgga
     2641 tcaactttat tttcttcagc cactcttcca atttcctcac tttcattttc aaaaatcaca
     2701 aaaatgatat taacttaaaa gccactaaca aaataagact tacaaagtaa agggtaatcc
     2761 aacaaaataa ccattcgctc tacgtactac gatttggtag tcctgccttt caatcggtaa
     2821 ttcataatag taatttactg tttcttcttt gccactttca atatactctg gattatccaa
     2881 tatcatagca tactcataaa gttcttcacc atcctt
//
LOCUS       DACGLE010000143         2914 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0534
            SAMEA3304449-rid9560453.denovo.154, whole genome shotgun sequence.
ACCESSION   DACGLE010000143 DACGLE010000000
VERSION     DACGLE010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304449
            Sequence Read Archive: ERR985064
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2914)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2914)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702225.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702225.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 20:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,045
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 27 of 67
            Pseudo Genes (incomplete)         :: 25 of 67
            Pseudo Genes (internal stop)      :: 19 of 67
            Pseudo Genes (multiple problems)  :: 4 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2914
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304449-rid9560453.denovo.154"
                     /strain="VREN0534"
                     /db_xref="taxon:1352"
     gene            255..1223
                     /locus_tag="IXT20_002765"
     CDS             255..1223
                     /locus_tag="IXT20_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-A"
                     /protein_id="HAQ4861166.1"
                     /translation="MNNIGITVYGCEQDEADAFHALSPRFGVMATIINANVSESNAKS
                     APFNQCISVGHKSEISASILLALKRAGVKYISTRSIGCNHIDTTAAKRMGITVDNVAY
                     SPDSVADYTMMLILMAVRNVKSIVRSVEKHDFRLDSDRGKVLSDMTVGVVGTGQIGKA
                     VIERLRGFGCKVLAYSRSRSIEVNYVPFDELLQNSDIVTLHVPLNTDTHYIISHEQIQ
                     RMKQGAFLINTGRGPLVDTYELVKALENGKLGGAALDVLEGEEEFFYSDCTQKPIDNQ
                     FLLKLQRMPNVIITPHTAYYTEQALRDTVEKTIKNCLDFERRQEHE"
     gene            1216..2247
                     /gene="vanA"
                     /locus_tag="IXT20_002766"
     CDS             1216..2247
                     /gene="vanA"
                     /locus_tag="IXT20_002766"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanA"
                     /protein_id="HAQ4861167.1"
                     /translation="MNRIKVAILFGGCSEEHDVSVKSAIEIAANINKEKYEPLYIGIT
                     KSGVWKMCEKPCAEWENDNCYSAVLSPDKKMHGLLVKKNHEYEINHVDVAFSALHGKS
                     GEDGSIQGLFELSGIPFVGCDIQSSAICMDKSLTYIVAKNAGIATPAFWVINKDDRPV
                     AATFTYPVFVKPARSGSSFGVKKVNSADELDYAIESARQYDSKILIEQAVSGCEVGCA
                     VLGNSAALVVGEVDQIRLQYGIFRIHQEVEPEKGSENAVITVPADLSAEERGRIQETA
                     KKIYKALGCRGLARVDMFLQDNGRIVLNEVNTLPGFTSYSRYPRMMAAAGIALPELID
                     RLIVLALKG"
     gene            2253..2861
                     /locus_tag="IXT20_002767"
     CDS             2253..2861
                     /locus_tag="IXT20_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-A"
                     /protein_id="HAQ4861168.1"
                     /translation="MEIGFTFLDEIVHGVRWDAKYATWDNFTGKPVDGYEVNRIVGTY
                     ELAESLLKAKELAATQGYGLLLWDGYRPKRAVNCFMQWAAQPENNLTKESYYPNIDRT
                     EMISKGYVASKSSHSRGSAIDLTLYRLDTGELVPMGSRFDFMDERSHHAANGISCNEA
                     QNRRRLRSIMENSGFEAYSLEWWHYVLRDEPYPNSYFDFPVK"
BASE COUNT          859 a          558 c          712 g          785 t
ORIGIN      
        1 tccagcgatg ccagacttgg ttgataaaag gaggtcctaa gagatgtata taatttttta
       61 ggaaaatctc aaggttatct ttactttttc ttaggaaatt aacaatttaa tattaagaaa
      121 cggctcgttc ttacacggta gacttaatac cgtaagaacg agccgttttc gttcttcaga
      181 gaaagatttg acaagattac cattggcatc cccgttttat ttggtgcctt tcacagaaag
      241 ggttggtctt aattatgaat aacatcggca ttactgttta tggatgtgag caggatgagg
      301 cagatgcatt ccatgctctt tcgcctcgct ttggcgttat ggcaacgata attaacgcca
      361 acgtgtcgga atccaacgcc aaatccgcgc ctttcaatca atgtatcagt gtgggacata
      421 aatcagagat ttccgcctct attcttcttg cgctgaagag agccggtgtg aaatatattt
      481 ctacccgaag catcggctgc aatcatatag atacaactgc tgctaagaga atgggcatca
      541 ctgtcgacaa tgtggcgtac tcgccggata gcgttgccga ttatactatg atgctaattc
      601 ttatggcagt acgcaacgta aaatcgattg tgcgctctgt ggaaaaacat gatttcaggt
      661 tggacagcga ccgtggcaag gtactcagcg acatgacagt tggtgtggtg ggaacgggcc
      721 agataggcaa agcggttatt gagcggctgc gaggatttgg atgtaaagtg ttggcttata
      781 gtcgcagccg aagtatagag gtaaactatg taccgtttga tgagttgctg caaaatagcg
      841 atatcgttac gcttcatgtg ccgctcaata cggatacgca ctatattatc agccacgaac
      901 aaatacagag aatgaagcaa ggagcatttc ttatcaatac tgggcgcggt ccacttgtag
      961 atacctatga gttggttaaa gcattagaaa acgggaaact gggcggtgcc gcattggatg
     1021 tattggaagg agaggaagag tttttctact ctgattgcac ccaaaaacca attgataatc
     1081 aatttttact taaacttcaa agaatgccta acgtgataat cacaccgcat acggcctatt
     1141 ataccgagca agcgttgcgt gataccgttg aaaaaaccat taaaaactgt ttggattttg
     1201 aaaggagaca ggagcatgaa tagaataaaa gttgcaatac tgtttggggg ttgctcagag
     1261 gagcatgacg tatcggtaaa atctgcaata gagatagccg ctaacattaa taaagaaaaa
     1321 tacgagccgt tatacattgg aattacgaaa tctggtgtat ggaaaatgtg cgaaaaacct
     1381 tgcgcggaat gggaaaacga caattgctat tcagctgtac tctcgccgga taaaaaaatg
     1441 cacggattac ttgttaaaaa gaaccatgaa tatgaaatca accatgttga tgtagcattt
     1501 tcagctttgc atggcaagtc aggtgaagat ggatccatac aaggtctgtt tgaattgtcc
     1561 ggtatccctt ttgtaggctg cgatattcaa agctcagcaa tttgtatgga caaatcgttg
     1621 acatacatcg ttgcgaaaaa tgctgggata gctactcccg ccttttgggt tattaataaa
     1681 gatgataggc cggtggcagc tacgtttacc tatcctgttt ttgttaagcc ggcgcgttca
     1741 ggctcatcct tcggtgtgaa aaaagtcaat agcgcggacg aattggacta cgcaattgaa
     1801 tcggcaagac aatatgacag caaaatctta attgagcagg ctgtttcggg ctgtgaggtc
     1861 ggttgtgcgg tattgggaaa cagtgccgcg ttagttgttg gcgaggtgga ccaaatcagg
     1921 ctgcagtacg gaatctttcg tattcatcag gaagtcgagc cggaaaaagg ctctgaaaac
     1981 gcagttataa ccgttcccgc agacctttca gcagaggagc gaggacggat acaggaaacg
     2041 gcaaaaaaaa tatataaagc gctcggctgt agaggtctag cccgtgtgga tatgttttta
     2101 caagataacg gccgcattgt actgaacgaa gtcaatactc tgcccggttt cacgtcatac
     2161 agtcgttatc cccgtatgat ggccgctgca ggtattgcac ttcccgaact gattgaccgc
     2221 ttgatcgtat tagcgttaaa ggggtgataa gcatggaaat aggatttact tttttagatg
     2281 aaatagtaca cggtgttcgt tgggacgcta aatatgccac ttgggataat ttcaccggaa
     2341 aaccggttga cggttatgaa gtaaatcgca ttgtagggac atacgagttg gctgaatcgc
     2401 ttttgaaggc aaaagaactg gctgctaccc aagggtacgg attgcttcta tgggacggtt
     2461 accgtcctaa gcgtgctgta aactgtttta tgcaatgggc tgcacagccg gaaaataacc
     2521 tgacaaagga aagttattat cccaatattg accgaactga gatgatttca aaaggatacg
     2581 tggcttcaaa atcaagccat agccgcggca gtgccattga tcttacgctt tatcgattag
     2641 acacgggtga gcttgtacca atggggagcc gatttgattt tatggatgaa cgctctcatc
     2701 atgcggcaaa tggaatatca tgcaatgaag cgcaaaatcg cagacgtttg cgctccatca
     2761 tggaaaacag tgggtttgaa gcatatagcc tcgaatggtg gcactatgta ttaagagacg
     2821 aaccataccc caatagctat tttgatttcc ccgttaaata aacttttaac cgttgcacgg
     2881 acaaactata taagcggttc tgttgcaaag tttt
//
LOCUS       DACGLE010000144         2887 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0534
            SAMEA3304449-rid9560453.denovo.155, whole genome shotgun sequence.
ACCESSION   DACGLE010000144 DACGLE010000000
VERSION     DACGLE010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304449
            Sequence Read Archive: ERR985064
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2887)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2887)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702225.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702225.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 20:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,045
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 27 of 67
            Pseudo Genes (incomplete)         :: 25 of 67
            Pseudo Genes (internal stop)      :: 19 of 67
            Pseudo Genes (multiple problems)  :: 4 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2887
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304449-rid9560453.denovo.155"
                     /strain="VREN0534"
                     /db_xref="taxon:1352"
     gene            <1..129
                     /locus_tag="IXT20_002768"
     CDS             <1..129
                     /locus_tag="IXT20_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HAQ4861169.1"
                     /translation="SGITMEKTKKGLLKQWDRKSHELFISILKELEEHENWYAEVE"
     gene            130..678
                     /locus_tag="IXT20_002769"
     CDS             130..678
                     /locus_tag="IXT20_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4861170.1"
                     /translation="MADIKGLDIFGNNKGLAKIKKEAEAKKKQQAITPSENKQLEVHQ
                     VQEIKTSSNVERQSRGRTKKVGAPIRKFDKVYSTKHPLKLSTLLNATSRVLVEKYETN
                     MTRDELLRKALDEYIKQNLTQEDKQDLFNDVVKELDLFREKYPIVPELDDRGEIIRTT
                     SQIKQDTEDSLRKGWGMNNKVR"
     gene            938..1666
                     /locus_tag="IXT20_002770"
     CDS             938..1666
                     /locus_tag="IXT20_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HAQ4861171.1"
                     /translation="MKKFKKKIYLGLVVGVSMFGFMAHTKQVTADTNQKERIMSQLDD
                     SQIEYVPNELIVSLKATQRISTEKNKPQNLLGVKVMVEKDLTLRTNESDYNKRDQVLL
                     VKLNEEISVEEAVRTFNASDMVNYAEPNYVVELEPLNDVEEPNTTDSLGNRSIILNDP
                     NWSKQANLREISMPSAWEETKGSKDVKVAVIDSGIDYQHEDLSGNVDESLRYDFFDDD
                     TDPMDTMGHGHILPGLLVLKQTTN"
     gene            1696..2823
                     /locus_tag="IXT20_002771"
     CDS             1696..2823
                     /locus_tag="IXT20_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HAQ4861172.1"
                     /translation="MVPIRVFGSSGGTSLAILTEAINYASQKGFPIINFSIGSVVYSS
                     TEEAIKNYPGLFVAAAGNNGIDMTDKPFYPASLDIPNIITVGNSSGYERSSDSNYSKT
                     GVDLFARGFRVFSTFLGSKYTYMSGTSMATPHVTGSAALALSLDPDLSTDELKKIILN
                     SVDSFYELSDLCLTGGRLNTNNVVRQVTGKLTASNITLRLNSEWSDEIAKEMCHVHWI
                     DQSGNDLTDQVVVLKNEVDTSNFGDYEVVFASPDLTDQISVTVTVPRLRKLTLGKETA
                     TIDIGSAWDSVIAIDTFQVKAIDDFGNDVTNQVQVTINADIDTINSIQAVIKFEVPEL
                     GLAQIGRLNITAKNSTINGLNEVSIEHVKKIMSFSKKNLDI"
     gene            2787..2887
                     /locus_tag="IXT20_002772"
                     /pseudo
     CDS             2787..2887
                     /locus_tag="IXT20_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360751.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
BASE COUNT         1047 a          393 c          616 g          831 t
ORIGIN      
        1 tcaggtatta cgatggagaa gacaaagaaa ggtctcttaa aacaatggga tcgcaaaagc
       61 cacgaattat ttatcagtat tttgaaagaa ttggaagaac acgagaattg gtatgcggag
      121 gtggagtaat tggctgacat caaaggactt gatatttttg ggaacaacaa aggactcgcc
      181 aaaataaaaa aagaagcaga agcaaagaaa aagcagcaag caattacacc atctgaaaat
      241 aagcaattag aagtgcacca agttcaagaa ataaaaactt cctccaatgt tgaaagacag
      301 agtagaggaa gaacaaaaaa agttggcgca ccaatcagaa agtttgataa ggtttattcc
      361 accaagcatc ctttaaaact gtcgactttg ttaaatgcga cttccagagt tttagtagaa
      421 aagtatgaga ctaatatgac aagagatgaa ttgcttagaa aagctttaga tgagtatatt
      481 aaacagaatc ttacgcaaga agataagcaa gatttgttta atgacgtggt gaaagaacta
      541 gacttgttca gagaaaaata tccaattgtt ccagaattag atgatcgtgg agaaataata
      601 agaactacca gtcagataaa acaagatacc gaagactcac tgcgcaaagg gtggggaatg
      661 aataataaag ttagataata atcaaagaag aactaagtaa ctacctttaa acggtagtta
      721 cttagttctt ctttgatttg cgtgaaatag aggggattta tttttctggt tacgtgatta
      781 aaaaatccaa aaaatgagca taggaacggt tatgatttta gggtgtgttt taatgagata
      841 ttgtgatttc attaacaagt gtataataat ctgttgttga gttagtaaat aagtaataga
      901 gctaactcta aaataaaact attaggagga gatgtaggtg aaaaagttta aaaagaaaat
      961 atatttagga ttggttgttg gggtgagtat gtttgggttc atggctcata ctaaacaagt
     1021 aactgcagat actaaccaaa aagaaaggat tatgagccaa ttagatgact ctcaaataga
     1081 gtatgtaccc aatgagctaa ttgtcagttt aaaggctaca caaagaattt caacagagaa
     1141 aaataaaccg caaaatttat tgggtgtaaa agttatggtg gaaaaagatt tgacccttag
     1201 gacaaatgaa agcgattata ataagcgaga tcaagtatta ttagtgaaat tgaacgaaga
     1261 aattagtgtg gaagaggcag taagaacatt taatgcttct gatatggtca attatgcaga
     1321 gccaaactat gtggttgaac tagagccgtt gaatgatgtt gaggagccaa atacaactga
     1381 ttctttagga aatcgatcaa ttattcttaa cgatcctaat tggagtaaac aagctaattt
     1441 aagagagatt agcatgccat ccgcttggga agaaaccaag ggttctaagg atgtgaaggt
     1501 agcggtcatt gattcaggaa ttgattatca acatgaagat ctttctggaa atgtagatga
     1561 aagtttgaga tacgattttt ttgacgatga tacagatcct atggatacta tgggacatgg
     1621 acacatattg ccgggattat tggtgctcaa acaaacaacg aattaggtgt tgctggaata
     1681 aattgggatg ttacgatggt acctatacgt gtatttggta gtagtggagg taccagtttg
     1741 gcaatcttaa ctgaagctat taattatgca tcccaaaaag ggtttccaat tatcaatttt
     1801 agtataggta gtgttgttta tagcagtaca gaagaagcaa ttaaaaacta tcctggatta
     1861 ttcgtagcag cagctggaaa taatgggata gatatgactg ataaaccttt ttacccagct
     1921 tctttggata tacctaatat tattactgta ggaaattcta gtggttatga acgaagttct
     1981 gattcaaatt atagtaaaac aggtgttgat ttattcgcta gagggtttcg tgtttttagt
     2041 acatttctag gaagtaaata cacgtatatg tcaggtacct cgatggcaac acctcatgtc
     2101 actggtagtg ctgcattagc gttgtcacta gatcctgatc tatctactga tgaattaaaa
     2161 aaaatcattt taaatagtgt tgattcattt tatgaactat ctgatttatg tcttacaggg
     2221 ggaagactaa atacgaataa tgtcgtacga caagtaacgg gtaaacttac tgcatctaat
     2281 atcacattaa gattaaatag tgaatggtca gatgaaattg ctaaagagat gtgtcacgtt
     2341 cactggattg accagtcagg aaatgatttg acggatcaag tggttgttct aaaaaacgaa
     2401 gtggatacat ctaactttgg cgattacgaa gtagtatttg catcacctga tttaacagat
     2461 caaatctcag ttacagtgac cgtacctcga ttacgtaaac ttacgctagg aaaagaaaca
     2521 gcaaccattg atattggttc cgcatgggat agtgtcatag ccattgatac ttttcaagtg
     2581 aaagctatag atgattttgg gaatgatgtt acaaatcaag tacaggttac tattaatgct
     2641 gatatagata caattaattc aattcaagca gtgattaagt ttgaagttcc agaattaggc
     2701 ttagcccaaa ttggtagact gaatattact gcaaaaaata gtacaatcaa tggattgaat
     2761 gaagttagca tcgagcacgt aaaaaagatt atgtcgtttt cgaagaagaa tttggacatt
     2821 tagagggtga tacgattgtc ggcatccacc ataaaagtgt cgtcatcaca ctcgttgagc
     2881 gactttc
//
LOCUS       DACGLE010000145         2766 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0534
            SAMEA3304449-rid9560453.denovo.156, whole genome shotgun sequence.
ACCESSION   DACGLE010000145 DACGLE010000000
VERSION     DACGLE010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304449
            Sequence Read Archive: ERR985064
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2766)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2766)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702225.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702225.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 20:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,045
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 27 of 67
            Pseudo Genes (incomplete)         :: 25 of 67
            Pseudo Genes (internal stop)      :: 19 of 67
            Pseudo Genes (multiple problems)  :: 4 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2766
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304449-rid9560453.denovo.156"
                     /strain="VREN0534"
                     /db_xref="taxon:1352"
     gene            124..717
                     /locus_tag="IXT20_002773"
     CDS             124..717
                     /locus_tag="IXT20_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAQ4861173.1"
                     /translation="MKDKIQCIFDKHNGNLSLKDARSEGLSPMTLDRLAEKDKIEKVA
                     PGFYTLKEAFVDELYLAQSIYSRGIFSHETALDLFQVGTYIPKKINMMFPKGYNVSKE
                     KLDEYAIQPHYTNKENFDLGITETESFYGNKILVYDLERTLCDMWNPRYKASVEVKQE
                     ALKEYMTSTSRNTNKLRRYMDILKSPKEMTLYMLPLY"
     gene            733..1632
                     /locus_tag="IXT20_002774"
     CDS             733..1632
                     /locus_tag="IXT20_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HAQ4861174.1"
                     /translation="MTPTQFKAKTRNFATEKHLNPQIVQRYYMMEKLLERIVESPYHD
                     DFVLKGGFLIGSKYGLENRSTVDIDTTYRNSKLTKEKLETVFSELTADPTKDGIRFEL
                     KGLTETREADYYPGFQAKFIAYLENARVPFKLDITSGDSIIPAALMYEHKLMFEDKTI
                     HIPAYPTEQILAEKLHATFSFGKDNSRMKDFYDLYMIPKLERINEKNLYKSVQGTFKE
                     RNNQSVFKHLYDQEMPQLVKDEGLREKWKRYQQENYFAKDISFESTLTSVDSLMQSIV
                     EQEKVERKQNFKRSQQMNQEMER"
     gene            1635..2120
                     /gene="ssb"
                     /locus_tag="IXT20_002775"
     CDS             1635..2120
                     /gene="ssb"
                     /locus_tag="IXT20_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HAQ4861175.1"
                     /translation="MINNVTLVGRLTKDPDLRYTASGTAVATFTLAVNRNFANQNGNR
                     EADFINCVIWRKPAETMATLAKKGGLIGVVGRIQTRTYDNQQGQRVYVTEVVADNFQL
                     LESKAATESRAHADQSSTSPSTATFEQRDTATPNNNGLNASQNPFGGQSIDISDDDLP
                     F"
     gene            2165..2398
                     /locus_tag="IXT20_002776"
     CDS             2165..2398
                     /locus_tag="IXT20_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4861176.1"
                     /translation="MDLLIPVIAFIDGISLGVTENPLVNRIAKGSLFLVFVYLVCSYL
                     LKRAGRDNSFWRLTKVTIAATAVILTAILPLYM"
     gene            complement(2557..2679)
                     /locus_tag="IXT20_002777"
     CDS             complement(2557..2679)
                     /locus_tag="IXT20_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002389461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative holin-like toxin"
                     /protein_id="HAQ4861177.1"
                     /translation="MKGVSLLSAYETIQTILGFGMFTIALISLIVNMLKNDKKK"
BASE COUNT          997 a          456 c          546 g          767 t
ORIGIN      
        1 tgttaaagaa ccagcagcgg attcaaaaag aaattgaatt agatcgataa atttctttct
       61 tgttaatata aaactaacat gttatagtta tgttagtttt atattaactt taggggaaaa
      121 gcaatgaaag acaaaatcca gtgtattttt gataaacata atgggaatct atccttgaag
      181 gatgctagaa gtgaaggatt atcgcctatg acgttagatc gattagccga aaaggacaaa
      241 atagaaaagg tagcaccagg tttctatacc ttaaaagaag cgttcgtgga tgaattgtat
      301 ctcgctcaaa gtatctattc cagagggatt ttttctcatg agactgcgct cgatcttttt
      361 caagtaggaa cgtatatccc taaaaagatc aatatgatgt ttcctaaagg atacaatgtt
      421 tcaaaggaga aattagacga gtatgctatt caaccgcatt acacgaataa agaaaatttt
      481 gacctgggaa tcacagaaac agaaagcttt tacggcaata aaattttagt ctatgattta
      541 gagcgaacgt tatgtgacat gtggaatcca agatacaaag caagcgtaga ggtaaaacaa
      601 gaagcattaa aagaatatat gacaagtacc agtagaaata caaataagct aagaagatac
      661 atggatattt taaaatcacc aaaagaaatg actttgtata tgttgccact ttattgagag
      721 gagtaaaggt gaatgacacc gacgcaattt aaagcaaaaa cgagaaattt tgcaacagag
      781 aaacatttaa acccacaaat tgtccaaaga tattatatga tggaaaaatt attagaacgt
      841 attgttgaat cgccttatca tgatgatttt gtattaaaag ggggatttct gatcggatca
      901 aaatacgggt tagaaaaccg ttcaactgtt gatattgata cgacttatcg aaattcgaaa
      961 ctaacaaaag aaaagttaga aactgttttt tctgaattaa cagctgatcc cacaaaagat
     1021 ggaattcgat ttgaattaaa gggattaaca gagacgaggg aagctgatta ttacccaggt
     1081 tttcaagcaa agtttattgc ttatcttgag aatgcacgtg taccatttaa attagatatt
     1141 acttcaggtg attctattat tccagcggca ttgatgtatg aacacaaatt aatgtttgaa
     1201 gataaaacga ttcatattcc agcttatcct acggaacaaa ttctagctga aaaattacat
     1261 gccacgttta gttttggaaa agataattcg cgcatgaaag atttttatga cctgtatatg
     1321 attccgaaat tagaacgtat caatgagaaa aatttgtata aaagtgttca aggaacattc
     1381 aaggaaagaa ataatcaatc ggtgtttaag catttgtatg accaggaaat gcctcaattg
     1441 gtgaaggatg aggggttgag ggaaaagtgg aaaagatacc aacaagaaaa ctattttgcc
     1501 aaagacattt ctttcgaatc tacactaact tccgttgatt ccctgatgca atcaattgta
     1561 gagcaagaga aggtagaacg aaaacaaaat tttaagagat cacaacaaat gaaccaagaa
     1621 atggagagat aaagatgatt aataatgtaa ctttagtagg acggttaacc aaagatccag
     1681 atttacgtta taccgcaagt ggcacagcag tagcgacttt taccctagcc gtcaaccgaa
     1741 atttcgcgaa tcaaaacggc aatcgagaag ccgattttat taactgcgtg atttggcgca
     1801 aaccagcaga aacaatggcg acattagcta aaaaaggcgg cttgatcggt gttgtcggac
     1861 ggattcaaac acgaacttat gacaaccagc aaggacaacg tgtgtatgtg actgaagttg
     1921 tagcagataa ctttcaacta ttagaaagta aagcagcgac agagagccgt gcgcacgctg
     1981 atcaatcgtc caccagtcca agcaccgcca cctttgaaca acgggataca gcgacgccta
     2041 acaacaatgg attgaacgct tcacagaatc catttggtgg acaatcaatc gatattagcg
     2101 atgatgatct accgttttag ttcgagaaag aaggagtaag catggcaaaa aaaatagtct
     2161 gacgatggat ctattgatac ccgtaattgc atttatagac gggatttctt tgggcgtcac
     2221 tgaaaatcct ttggtaaata gaattgcgaa aggatcacta tttcttgtat tcgtgtatct
     2281 tgtatgttct tatcttttaa aaagagcagg aagggataat tccttttggc gattgactaa
     2341 agtcacaata gcagcgacag ctgttatact tacggctatt ctaccgcttt atatgtaggg
     2401 ttaacgggaa atcgttgaac ttcttgcgaa atattgctat aatgaacatg aaaaagagga
     2461 gttgcgctaa caacccctca ttaacagcac cgattaaaga acggtggcag aatattagtt
     2521 atttggaaat aaccgctgac ctgctaaagt ggacggttat ttctttttgt catttttaag
     2581 catatttaca atcaaactaa tcaaagcaat ggtaaacata ccaaaaccaa gaatggtttg
     2641 aattgtctca tatgcggaca aaaggcttac tcctttcata gattttagca cttacattca
     2701 taagcaccac cacctttcca gaagtcgcca ccgtcttcaa actttactgt cttgactatt
     2761 atagca
//
LOCUS       DACGLE010000146         2719 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0534
            SAMEA3304449-rid9560453.denovo.157, whole genome shotgun sequence.
ACCESSION   DACGLE010000146 DACGLE010000000
VERSION     DACGLE010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304449
            Sequence Read Archive: ERR985064
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2719)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2719)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702225.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702225.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 20:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,045
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 27 of 67
            Pseudo Genes (incomplete)         :: 25 of 67
            Pseudo Genes (internal stop)      :: 19 of 67
            Pseudo Genes (multiple problems)  :: 4 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2719
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304449-rid9560453.denovo.157"
                     /strain="VREN0534"
                     /db_xref="taxon:1352"
     gene            700..2610
                     /gene="ltrA"
                     /locus_tag="IXT20_002778"
     CDS             700..2610
                     /gene="ltrA"
                     /locus_tag="IXT20_002778"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HAQ4861178.1"
                     /translation="MPKNKNETLCVEDLRHAEYYEMQNTFDDLYAKSKNGDIFTHLMD
                     IILSRENILLAYRNIKANAGSKTAGTDGTIIKDIGKLPAETVVKKVRYIVAGSPHGYR
                     PKPVRRKEIPKPNGKTRPLGIPCMWDRLIQQCIKQVLEPICEAKFSENSYGFRPNRSV
                     ENAIKATYNRLQISQLHYVIEFDIKGFFDNVNHSKLIKQIWAMGIRDKHLIFILKRIL
                     KAPIKMTNGTITYPEKGTPQGGIISPLLANIVLNELDHWVESQWQENPVTKNYVVHIN
                     KSGSPCKSNAYKEMKKTKLKEMYMVRYADDFRVFCRYKESAEKAKIAITQWIEQRLKL
                     EVSQEKTRIVNVRKRYSDFLGFKIKMIPRRKKLVVKSHISDKQFKNQKDKLVSQAKKI
                     ASPSKGKTELEETRLYNSMVLGMQNYYQIATNVNLDCMKLNRAVMAIFTNRLGTNKRG
                     RLRKTGRQLTTKEATRYGKSKMLRYVAGEGEPIFPVGYIQHRKPMAKVYLANCYTLEG
                     RKFIHTNLSVDKLLMYQLMKLSLENRTIEYADNRISLFSAQHGKCAITLEEFQQANEI
                     HCHHIVPTGAGGNDDYKNLILVKEAVHRLIHAKTEETIQKYIALLKLNKVQLIRLNKY
                     RVLAGNQELNLI"
BASE COUNT         1065 a          420 c          556 g          678 t
ORIGIN      
        1 aaaagctaag gcactaataa aagaacgaaa aagtttggtt attaggagaa ctgataatct
       61 gacaggtgga atgatgaagt aacgctctga aacgcctgcc ctgagcctcc gacaagcatt
      121 gtatgaggaa actcagaatg tttgaagctc ggtaaagacg gctgaaagat accgtaatag
      181 ttgatgaaca tatatcttct atcgaaaact atccagaggt gggtagcgta tccaacaggt
      241 cgggggtcta taaaatatct atggttagaa tgttctataa ggaactgacg aaacagcgaa
      301 tgtacgggtc taaatttgaa ctttgtagaa atgcaaaggt acgagaaatc gtagctggtg
      361 tggcaaagta agaatatagg ttatgaaatg ccatataacg ttacaggcgg tatcaagcca
      421 gcaggcttat agctagtgac ctaaggatat atatgtatag ataagattat cggaacgagg
      481 aaaggtattg ggttctcatc aagagaataa actgacgaag aacaataagc agttgaacta
      541 atgctgaaaa gcagagggcg aacctaagaa attcttgtaa tgagaatgga ggaatgccct
      601 caagtcgtgt atatattaga aaagtattat gttgtttcaa taaggattac gagtaagata
      661 aaaaagctca ctcaatttca agtaaaggag tgaagcctta tgcctaaaaa taaaaatgaa
      721 acattgtgtg tagaagattt aagacacgct gaatattacg aaatgcaaaa cacctttgat
      781 gatttatatg ccaaaagtaa gaatggagat attttcactc atcttatgga tattatccta
      841 tcgagagaaa atatattact ggcatatcga aatatcaaag caaatgcagg cagtaaaaca
      901 gcaggaacag acggtacaat aattaaagat attggcaaat tgccagcaga aacagttgtc
      961 aaaaaagtaa gatacattgt tgcaggcagt ccacatgggt atcgacctaa gccagtgagg
     1021 cgaaaagaaa taccaaaacc aaatggtaaa accagacctc taggtattcc ttgtatgtgg
     1081 gacaggctta tacaacaatg tataaaacaa gtattagaac caatctgcga agctaaattt
     1141 agtgaaaaca gctatggttt tagacctaat aggtcagttg aaaatgcaat aaaagcaacc
     1201 tataaccgcc tacaaatcag ccagctacat tatgtgattg aatttgatat taagggtttc
     1261 tttgacaacg tgaaccattc caagctgata aaacagatat gggctatggg aatacgggac
     1321 aagcacttga tattcatttt aaaaaggata ttaaaagctc caatcaaaat gactaacggt
     1381 acaataacat atccagagaa aggaactccg caagggggaa taatatcccc actactagca
     1441 aacattgttc ttaatgagtt agaccattgg gtagaaagcc aatggcagga aaatcctgtt
     1501 acaaaaaact atgtggttca tatcaataaa agcggtagcc catgtaaaag caacgcttac
     1561 aaagaaatga agaaaacaaa gttaaaggaa atgtatatgg ttcgttatgc agatgatttt
     1621 agagttttct gccgctacaa ggaaagtgct gaaaaagcca aaatagcaat cactcaatgg
     1681 attgaacaac gattaaaatt ggaagtatca caagaaaaaa caagaattgt aaatgttaga
     1741 aaaagatact ccgactttct tggtttcaag attaaaatga taccgagaag aaagaagtta
     1801 gttgttaaat cacatataag tgataaacaa ttcaaaaacc aaaaagacaa gctggtatca
     1861 caggcaaaga aaattgcttc tccctcaaaa gggaaaacgg aattagaaga aacaagactt
     1921 tataattcaa tggtattagg tatgcaaaat tattatcaga ttgcaacgaa tgtaaatctg
     1981 gattgtatga aactgaatag agctgtaatg gctatcttca ctaacagatt aggtacaaac
     2041 aaaaggggca gattgagaaa aacaggcaga caacttacga caaaggaggc aacaagatat
     2101 ggtaaatcta aaatgcttcg gtatgttgct ggcgagggcg aacctatttt ccctgttgga
     2161 tatatccaac acagaaaacc aatggcaaag gtgtatttag cgaattgcta cactctagag
     2221 ggaagaaaat ttatccacac caatctaagt gttgataaat tactgatgta ccagttaatg
     2281 aaattatctt tggaaaatcg cacaatagaa tacgcagaca atcgaatttc gctattttct
     2341 gctcaacatg gaaaatgtgc tataactctg gaagaatttc aacaagctaa tgaaatacat
     2401 tgtcatcata ttgtaccaac tggagctggt ggaaatgacg attataaaaa tcttattttg
     2461 gtaaaagagg cggtacaccg tctaatacac gctaaaacag aagaaacaat acaaaaatat
     2521 atagctctat tgaagctaaa taaagtccag ctcataagac taaataaata tagagtatta
     2581 gctggcaatc aagaattaaa cttgatatag gtctactaca taaatacttt tcaatataaa
     2641 cacgatggaa cgcggagtgc ggtgaaagtc gcacgctccg tgtgaatggg gggaaaagtt
     2701 tgagatattt ataatatca
//
LOCUS       DACGLE010000147         2633 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0534
            SAMEA3304449-rid9560453.denovo.158, whole genome shotgun sequence.
ACCESSION   DACGLE010000147 DACGLE010000000
VERSION     DACGLE010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304449
            Sequence Read Archive: ERR985064
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2633)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2633)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702225.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702225.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 20:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,045
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 27 of 67
            Pseudo Genes (incomplete)         :: 25 of 67
            Pseudo Genes (internal stop)      :: 19 of 67
            Pseudo Genes (multiple problems)  :: 4 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2633
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304449-rid9560453.denovo.158"
                     /strain="VREN0534"
                     /db_xref="taxon:1352"
     gene            5..1149
                     /locus_tag="IXT20_002779"
                     /pseudo
     CDS             5..1149
                     /locus_tag="IXT20_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328567.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
     gene            1320..1778
                     /locus_tag="IXT20_002780"
     CDS             1320..1778
                     /locus_tag="IXT20_002780"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HAQ4861179.1"
                     /translation="MRMNVESFNLDHTKVKAPYVRVADRKTGAHGDVIIKYDVRFKQP
                     NKEHMDMPSLHSLEHLTAELIRNHADYIVDWSPMGCQTGFYLTVLNHESYEDILDVLE
                     ATMKDVLEATEVPASNETQCGWAASHTLEGAQALAREFLAKRDEWSEVEA"
     gene            1937..2407
                     /locus_tag="IXT20_002781"
     CDS             1937..2407
                     /locus_tag="IXT20_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HAQ4861180.1"
                     /translation="MSIYNFETTLEDGTVYSLKKYAGKPIIIVNTATKCGLAPQFKQL
                     EELYQAYQTQGLVILGFPSNQFKQELDSATEAAEACKTTYGVTFPMHQLIDVNGENTD
                     PLFTYLKNEAPGTVTKAIKWNFTKFLIDRNGKVIERFAPQTSPLKMTAAIESVL"
     gene            complement(2460..2575)
                     /gene="rrf"
                     /locus_tag="IXT20_002782"
     rRNA            complement(2460..2575)
                     /gene="rrf"
                     /locus_tag="IXT20_002782"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT          863 a          504 c          496 g          770 t
ORIGIN      
        1 aaacatgcac attcaaacaa aattgatcca cggaggcatc agtgaagatc ccacaacagg
       61 agctgtcagt gtacctattt atcaaacatc cacttatcgg caagatggcg tcggccagcc
      121 taaacaatat gaatattcaa gatctggtaa tcctactcgt tttgcattgg aagaactgat
      181 tgctgatttg gaaggcggtg ttcgaggatt cgctttttct tctggtcttt ctgggattca
      241 tgccgttttt tctctttttc aagctggcga tcatatctta ttaggagatg atgtctatgg
      301 aggaacattc cgtctatttg acaaagttct tactaaaaat ggtctcgagt acacgatcat
      361 agatacgagt aatcttgata aaatagaaca atccatcaaa cccaatacaa aagcacttta
      421 tcttgaaaca cctagcaatc cgcttctaaa gattactgat ttagaaaaaa gtgccactct
      481 ggctcatcaa catggattga tagttattgc tgacaataca tttgctactc cttattttca
      541 aagaccttta gatctcggtt ctgatatcgt tgtccatagc ggtaccaaat atctaggcgg
      601 acacagcgat gtagtagctg gtttagtgac ttctaaccaa aagatctagc cgatcagatc
      661 ggattttacc aaaatgctat cggagccgta cttggtcccc aagatagctg gttacttcag
      721 cgcggaatca agacactaag tgttcgtatg gaggaacatc aaaaaaatgc ttttgtggta
      781 gctgattttc tattttctca tccagcagta gaaaaggtct actatccagg attacccgat
      841 catgaactcc atggagttgc taagcagcag atgagcggct ttagcggaat gatttctttt
      901 actttaaaaa atgaagaatc tgctattcca tttgttgaat cactccaact atttacttta
      961 gcagaaagtt taggtggcgt agaaagttta gtggaaattc cttctgtcat gactcatgcg
     1021 tctattccaa aagaaaaacg agaagaggct ggcataaagg acggcttgat ccgtctttct
     1081 gtaggaattg agtatggtca ggatctcata aacgatttag ctcaggcatt tgacagaatc
     1141 aagaactaga agtcccaata aacgacttag cagaaaaatc taaaaaatat ttcagattgc
     1201 tacaccttgg aaacgataag tgttagaatc aagtagaaag acgtttttgt aaatttcact
     1261 aaataattct ttagtgaaaa gcatcaaaac gaatgaacaa acagatagga gtcttacaaa
     1321 tgagaatgaa tgtcgaaagt tttaatcttg accatacaaa agtcaaagca ccttatgtac
     1381 gtgtagctga tcgaaaaacg ggtgcgcatg gcgatgtcat cattaaatac gacgtccgct
     1441 ttaaacaacc aaataaagaa cacatggaca tgccaagtct acattcactt gagcacttga
     1501 ctgccgaact aatccgcaat cacgcagatt acatcgtaga ctggtctcca atgggatgcc
     1561 aaacaggctt ttacttgact gtattgaatc atgaaagcta cgaagacatt ttagatgttc
     1621 ttgaagcaac aatgaaagat gttcttgaag ctactgaagt acctgcaagc aatgaaacac
     1681 aatgtggctg ggctgccagc catacactag aaggtgcaca agctttggct cgtgaatttt
     1741 tagcaaaacg cgatgaatgg tctgaagtag aagcatagtt tctaaaagaa cgtaaagatg
     1801 agaagcaata tagctatctt atcttcgtat tttattaaat atttattgtc tgaaaagagt
     1861 tctaaacggg aaaacaaata gctagttttc agtttagcgc tctttttatt atataaaata
     1921 gaaaggagaa aactttatgt ctatttataa ttttgaaact actttagaag acggcactgt
     1981 ctattcattg aaaaaatatg caggcaaacc gataatcatt gttaacactg caacaaaatg
     2041 cggtctagca cctcaattta aacaattaga agaactttac caagcttatc agacacaagg
     2101 tctggttatc ttaggctttc cttccaatca atttaaacaa gagctagatt cagcgactga
     2161 agcagcagaa gcttgtaaaa ccacttatgg cgttaccttc cctatgcatc aattgattga
     2221 cgtaaatgga gaaaatacag atcctctttt tacttatctt aaaaatgaag ctcctggtac
     2281 tgtcactaaa gcaataaaat ggaattttac taaatttttg attgatagaa atggcaaagt
     2341 aatcgaacga tttgcaccac aaacttctcc attgaaaatg accgctgcaa ttgaatccgt
     2401 tctttaactt aataaaaata aaaaaagatg aactatttct agttcatctt tttgcctttg
     2461 cgtggcgacg tcctactctc acaaggggca acccctcact acaatcggcg ctaagaagct
     2521 taacttctgt gttcggcatg gttacaggtg tatccttctc gctatcgcca ccacactgtg
     2581 gtgttatctt ttattgagta aattttgttc actcaaaact ggatttgaag tca
//
LOCUS       DACGLE010000148         2628 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0534
            SAMEA3304449-rid9560453.denovo.159, whole genome shotgun sequence.
ACCESSION   DACGLE010000148 DACGLE010000000
VERSION     DACGLE010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304449
            Sequence Read Archive: ERR985064
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2628)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2628)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702225.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702225.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 20:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,045
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 27 of 67
            Pseudo Genes (incomplete)         :: 25 of 67
            Pseudo Genes (internal stop)      :: 19 of 67
            Pseudo Genes (multiple problems)  :: 4 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2628
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304449-rid9560453.denovo.159"
                     /strain="VREN0534"
                     /db_xref="taxon:1352"
     gene            complement(1..595)
                     /locus_tag="IXT20_002783"
                     /pseudo
     CDS             complement(1..595)
                     /locus_tag="IXT20_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            1212..1430
                     /locus_tag="IXT20_002784"
                     /pseudo
     CDS             1212..1430
                     /locus_tag="IXT20_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391850.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            1690..1944
                     /locus_tag="IXT20_002785"
     CDS             1690..1944
                     /locus_tag="IXT20_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAQ4861181.1"
                     /translation="MGKELKVRKIGNSVGVILPSSLGLKSGDTIQAKQEGNLFILDTT
                     QIAKEHDRKLIEESFQDFEKGLTVSEIEMVKAFGKYGWSE"
     gene            1945..2262
                     /locus_tag="IXT20_002786"
     CDS             1945..2262
                     /locus_tag="IXT20_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HAQ4861182.1"
                     /translation="MKEYTLVYSKSFQTSLETIIEEWENELALSPASIKRFVGAIQKA
                     LELTKNFPTMHEEVSEIYGMDIPTYRIVIGKSYALFYRIDHEKKEILVGRIFQSKQMK
                     LGF"
BASE COUNT          886 a          436 c          490 g          816 t
ORIGIN      
        1 gattttcgac aatatccaat aatgcatggc tttgtccgtc catcatgata aaactcattg
       61 cactggcaac acttcgaacc gatttgaatt catcaaagca taagaccttt ggcaaagtct
      121 gtttgaaagg attcaatggg cggtagaatc gcttcatgac acgatagacc gaagaagtgg
      181 aaatattttt ctgacgagcg atttccgtca tggaagtatt ctcttttaag cgagcaatga
      241 cagaccaacg aactttttga ctaatgaaac agtgtttttc agcaacagag gtatccgcta
      301 cgaatgttct ttggcaagac ttacatttaa atcattgttt cttcagctcc agataagtgc
      361 gatactcaga cacatcattc agtaacaacc gaacggttgt cgtcccccaa cgaatgatcc
      421 tagaggggac gacacaaaga caatgggaac actcttctgg cttgtacaga agagttcctt
      481 tgtaaatcaa gcaaatttgc cctttgtatt tttctttagt taaacaattt tcctcgaaat
      541 agatatttag gtctagaata tttagggtat ctttgataag ctgagtatga gacatcttga
      601 atcctcctat gaattggtag ccaaattaat tctaaaggat ttcagggtgt cttttctata
      661 tttttttata caaaaaagat gttggtgaag ttcaccaaca tcaaaaagta tagagccttt
      721 cattatatca ccaaacccca tcagcgtgca ggctgatggg gtttttttac gtttctatat
      781 tatagaatta acgggtaggc ttactgtctt ttttggcaat tttgcaatca agccatttgc
      841 agaggtaact gcacactaca tctgcaaatg gcttgattgc aaaaacaaaa tcaaaaaggt
      901 atttcattat atctcctcct ccccttgaca gattaggccg gggtagcaga cagcaaaact
      961 attataacat aactgaaata cctaaatgac atatacagat atgataattt atgtattgat
     1021 ttttctaaca aaagcacata ttttaatata tactacctat tttaggtagt atatattttt
     1081 agatacaata tcgatcagtt ttgaggaaat gaaattctag atattttttg ctaaatagag
     1141 ttactttcaa tattttttaa cgtaattagg cagaagtcac tcatcctcta taggtttgtg
     1201 atgaaattgc cattgatcca tttaagtaag agcaaaaaac tggaggttta tcaggtaatt
     1261 ggtctaatgg atttaagtat gcggtcaccg tgtatagagt tgcttatgag attcaagaaa
     1321 ataaagttat tccagtattg ctatatggaa cacataacaa tttttatgaa ttagccgaga
     1381 acactcgcaa ctttattcgt gtagatgaat cggcttcggt tgagttataa ctttagctat
     1441 atcaatcaac cgaccgacat atgttaaatc tatatataat aaaaatacct acttaaatgg
     1501 atgttaacgg aagtgtatgg tgagtagttc actcgtattt tgatatcgtt tgtatatgtt
     1561 ttcagcgttc taagtggctt attttgtcca ataataatat ggagtggaat cccttaagaa
     1621 acaccgttgg caattgttgt tataacatgt tataatttat tcatcaaaaa ctaaaaggag
     1681 tgtgataaca tgggaaaaga actaaaggtt agaaagatcg gcaatagcgt gggagtgatc
     1741 cttccaagct ctcttggatt aaaaagcgga gacactatcc aagccaaaca agaaggcaac
     1801 ttattcatat tagatactac tcaaatagca aaagaacacg atcgcaaatt gattgaagaa
     1861 agtttccaag actttgagaa aggtttaaca gtttcagaaa ttgaaatggt aaaagctttc
     1921 ggaaaatacg gctggagtga atagatgaaa gagtacacgc tagtttattc taaaagtttc
     1981 cagaccagcc ttgaaacgat aatagaagaa tgggaaaatg agcttgctct gtctccagcc
     2041 agcataaaaa gatttgtagg tgcgatccag aaggctttag aactaaccaa aaattttcca
     2101 actatgcatg aggaagtttc cgaaatctat ggaatggata tcccaacata tcggatcgtg
     2161 atcggcaaaa gctatgcctt attttatcgg atcgaccatg aaaaaaaaga gatacttgta
     2221 ggacgtattt tccaatctaa acaaatgaaa ctaggctttt aacaataatt aggcaaaagt
     2281 cgctcatcct ctacaggtgg gtgattaatg ccgctcgata tggttgttaa ccgtcggtta
     2341 ctcgaagatt gatatataag acttagtacc tttttatttt gctccatgtt atggccgaag
     2401 gtctaaagat acaatcatta gtctagagaa aaattaagaa ccgtaggaac tacggggatc
     2461 gcttggtgaa taagagacac tgctattagt gaaaaaataa gatatcaagt acgctctatt
     2521 cccaaaaatt tcgtgacttt actcatgaga ggttcaagta gtatattttt aatcaaaatt
     2581 gacgtaactt ttaataaata ctacctaaat aaggtagtat ttattttt
//
LOCUS       DACGLE010000149         2484 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0534
            SAMEA3304449-rid9560453.denovo.161, whole genome shotgun sequence.
ACCESSION   DACGLE010000149 DACGLE010000000
VERSION     DACGLE010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304449
            Sequence Read Archive: ERR985064
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2484)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2484)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702225.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702225.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 20:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,045
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 27 of 67
            Pseudo Genes (incomplete)         :: 25 of 67
            Pseudo Genes (internal stop)      :: 19 of 67
            Pseudo Genes (multiple problems)  :: 4 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2484
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304449-rid9560453.denovo.161"
                     /strain="VREN0534"
                     /db_xref="taxon:1352"
     gene            complement(1014..1304)
                     /locus_tag="IXT20_002787"
     CDS             complement(1014..1304)
                     /locus_tag="IXT20_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4861183.1"
                     /translation="MEKKYGTVDKIRTLQVSARPLIRFSLNGVNCLIASHSLNFLAEV
                     DEGMKLVVTGYYNNRKQFVVRSYHVLGKPKIVVEYEKSLYPRKKVRQDERIN"
     gene            complement(1512..2204)
                     /locus_tag="IXT20_002788"
     CDS             complement(1512..2204)
                     /locus_tag="IXT20_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HAQ4861184.1"
                     /translation="MKKKFITGGIVVAFLCLIGYTGTRYANEIILWGGETDIDTINEN
                     LTQLDHTLTDKEQAITDLTARLAQKEQTLQDSKADVQEYQTKVNELENQKNQSAAEKQ
                     NLESQLVSKDAQLQSKQNEINVKLDEINQKNDEINHLNADWTARLQEAQAKLSELQNT
                     VNGLNQQIATLTQEKTNVETQLTDTQKKLDEATAENSSLKQYIQKLEKAKDEVEDTAD
                     KSQQIVEEHLRK"
BASE COUNT          734 a          457 c          349 g          944 t
ORIGIN      
        1 atacttgcaa agtaatacta caattcctaa cataatcgta gaatttaata atttccatag
       61 cataaagtgt ttagaaaaga acattaagaa tagttctata ataattcttg aactccatgt
      121 cttataatgt tctatcgaag cacccaaata gccaagttca tctaatctac ttgcataaac
      181 tacgtcatcc gctctcatag tcggcatata catattaaag agtaaaaaga taccccaaat
      241 ccctattagt aacaagatca gttctttgtt attcgttatt tttctattat gtttgttcat
      301 ttttatactc cttcttatat ttcctaatag agtataacat agctttaagc attacttaaa
      361 gataacttaa aagttatctt ttaaatactt aattgttaaa taccttattt gatgcaaaag
      421 aaatctacga tagtcaacaa aaaaattttc attatatcca gaaaaatttt tcttagttaa
      481 ggacctatgg attgcagtaa tgcagaattc gtcgaataaa ctgccaaaga catacaatca
      541 tattgctttt aaaattgacg aacaagatat agattcgttt gtctcaaaaa tccaaatgtt
      601 aggtttaact gtcgaaccag gacgttctcg tgtgaaaggc gaggcttctt caatttactt
      661 ttacgactat gataatcacc tcttcgagtt acataccggt actctccagg aacgtttaaa
      721 atcctacaat tccactacct gagactagta atgactagtc tctttttatg gcctctgatc
      781 cccccattga gcgaatacta atagggcttc gaattgatct gttcaatctt taagcaatat
      841 tcctcaaatg agtttggaaa gtttagtgat tcatcacaaa tacttaaata catgtagtta
      901 tatatagatt gaggttcttg tgagcgtatg ctcgtgtggg ttcaaatccc atcgtctgca
      961 tccatctttc caaaacaagc ggctaggaca tttttgtctc agccgcttgt tttctagttt
     1021 attctttcat cttgtcggac cttttttctt gggtatagac ttttctcata ttctaccaca
     1081 attttgggtt tccctaacac atgataggat ctcacaacaa attgctttct attattataa
     1141 taacctgtaa cgactaactt catcccttcg tctacttcag ctaggaagtt caaactatgg
     1201 gaagcaatca aacaattgac cccatttaaa ctgaaacgga tcaacggtct ggcactaact
     1261 tgcaaagtac ggatcttatc tactgttcca tattttttct ccataacctt cacctcaata
     1321 ccattatacg aacatacatt ccctttgtaa agaagaaact ctgttttatc tttttgactt
     1381 tttgtctatt acctttctta aaatcatatt ggcacttcct gcgaagatga aggacatgta
     1441 ctaaaaaaaa gaggcagcag tatagattac tgttgcttct ttactaattt aggtttactt
     1501 gttaagaaag attatttacg caaatgttct tcaacaattt gctgagattt atcagctgta
     1561 tcttccactt catcttttgc tttttctaac ttttgaatat attgtttcaa gcttgagttt
     1621 tcagcagttg cttcgtctaa tttcttttgt gtatctgtta actgagtttc tacatttgtt
     1681 ttttcttgtg tcagtgttgc gatttgttga ttcaaaccat ttacagtatt ttgtaattct
     1741 gaaagttttg cttgtgcttc ttgtagtctt gctgtccaat ctgcattcag atgattgatt
     1801 tcatcatttt tttggttgat ctcatctagt ttgacattga tttcattttg tttgctttgt
     1861 agttgtgcat ccttgctgac taattgactt tctaagtttt gtttttctgc tgccgattga
     1921 tttttttggt tttctaattc atttactttt gtttggtatt cttggacatc tgcctttgaa
     1981 tcttgcaatg tctgttcttt ttgtgctagt cgagcggtaa gatctgtaat cgcttgctct
     2041 ttatctgtca atgtatgatc aagctgtgtc agattctcat tgattgtatc aatatctgtc
     2101 tctccacccc acaaaataat ttcatttgcg taccgcgtgc ctgtgtagcc aattagacat
     2161 agaaatgcaa cgacaatccc accagtaata aatttctttt tcatttgcat agctccttaa
     2221 tccaatactt ttacatgcta aaagtaagtg atctcaaaca caatgtcaag cgtttttaaa
     2281 ttacatatgt aattttaata taaatactat caaatactca tttaatcctc cttttttatc
     2341 tatttaaaaa atctttttta tagagaaaaa tgcaggttaa tactttctta aataaatatt
     2401 tatggtgaac aaaaacaaaa aagcaagttt tattaaacgt aacccttttc atttcataga
     2461 ttactgtatt tattacaaaa aacg
//
LOCUS       DACGLE010000150         2474 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0534
            SAMEA3304449-rid9560453.denovo.162, whole genome shotgun sequence.
ACCESSION   DACGLE010000150 DACGLE010000000
VERSION     DACGLE010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304449
            Sequence Read Archive: ERR985064
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2474)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2474)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702225.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702225.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 20:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,045
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 27 of 67
            Pseudo Genes (incomplete)         :: 25 of 67
            Pseudo Genes (internal stop)      :: 19 of 67
            Pseudo Genes (multiple problems)  :: 4 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2474
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304449-rid9560453.denovo.162"
                     /strain="VREN0534"
                     /db_xref="taxon:1352"
     gene            <1..622
                     /locus_tag="IXT20_002789"
     CDS             <1..622
                     /locus_tag="IXT20_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015077585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS256-like element IS256 family transposase"
                     /protein_id="HAQ4861185.1"
                     /translation="HIAIGITKDGDREIIGFMIQSGESEETWTTFFEYLKERGLQGTE
                     LVISDAHKGLVSAIRKSFTNVSWQRCQVHFLRNIFTTIPKKNSKSFREAVKGIFKFTD
                     INLAREAKNRLIHDYIDQPKYSKACASLDDGFEDAFQYTVQGNSHNRLKSTNLIERLN
                     QEVRRREKIIRIFPNQTSANRLIGAVLMDLHDEWIYSSRKYINFDK"
     gene            complement(752..2191)
                     /locus_tag="IXT20_002790"
                     /note="aph(2'')-Ia"
     CDS             complement(752..2191)
                     /locus_tag="IXT20_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(2'')-Ia"
                     /protein_id="HAQ4861186.1"
                     /translation="MNIVENEICIRTLIDDDFPLMLKWLTDERVLEFYGGRDKKYTLE
                     SLKKHYTEPWEDEVFRVIIEYNNVPIGYGQIYKMYDELYTDYHYPKTDEIVYGMDQFI
                     GEPNYWSKGIGTRYIKLIFEFLKKERNANAVILDPHKNNPRAIRAYQKSGFRIIEDLP
                     EHELHEGKKEDCYLMEYRYDDNATNVKAMKYLIEHYFDNFKVDSIEIIGSGYDSVAYL
                     VNNEYIFKTKFSTNKKKGYAKEKAIYNFLNTNLETNVKIPNIEYSYISDELSILGYKE
                     IKGTFLTPEIYSTMSEEEQNLLKRDIASFLRQMHGLDYTDISECTIDNKQNVLEEYIL
                     LRETIYNDLTDIEKDYIESFMERLNATTVFEGKKCLCHNDFSCNHLLLDGNNRLTGII
                     DFGDSGIIDEYCDFIYLLEDSEEEIGTNFGEDILRMYGNIDIEKAKEYQDIVEEYYPI
                     ETIVYGIKNIKQEFIENGRKEIYKRTYKD"
     gene            complement(2192..>2474)
                     /locus_tag="IXT20_002791"
     CDS             complement(2192..>2474)
                     /locus_tag="IXT20_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000393259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ4861187.1"
                     /translation="IGFAYCYTLLRPDGKTMFYLHSIGMLPNYQDKGYGSKLLSFIKE
                     YSKEIGCSEMFLITDKGNPRACHVYEKLGGKNDYKDEIVYVYDYEKGDK"
BASE COUNT          842 a          434 c          254 g          944 t
ORIGIN      
        1 tcatatagcg attggaataa ccaaagatgg cgaccgtgaa attatcggct tcatgattca
       61 aagtggcgaa agcgaagaga cctggacaac attttttgaa tacctaaaag aacgcggttt
      121 acaaggtacg gaactcgtta tttctgatgc gcacaaagga ttagtctctg ccattagaaa
      181 atccttcacc aacgtaagtt ggcaaagatg ccaagttcac ttcctaagaa atatctttac
      241 caccattcct aaaaaaaatt caaaatcttt cagagaagct gttaaaggaa tttttaagtt
      301 cacagatatt aacttagcgc gtgaggctaa aaatcgattg attcatgatt atatcgatca
      361 accaaaatat tcaaaagctt gcgcatcatt ggatgatgga ttcgaagacg cctttcaata
      421 taccgtacaa ggaaattccc acaatcgact aaagagtacc aatctaattg aacgactgaa
      481 tcaagaagta cgcagaagag aaaagattat tcgcatcttc cccaatcaaa catcagccaa
      541 tcgcttaatt ggagccgttc ttatggacct acatgatgaa tggatttatt cttcaagaaa
      601 atacatcaat tttgataagt agaaatggta aaaacattgt atagcatttt acacaggagt
      661 ctggacttga ctcacttcct ttattatttt tcattttttt aaactattta atactaatgt
      721 cttttataat agcttttcat atattatata atcaatcttt ataagtcctt ttataaattt
      781 cttttctacc attttcgata aattcctgtt taatattttt aattccataa acaatagttt
      841 caataggata atattcttca actatatctt gatattcttt tgctttctca atatctatat
      901 ttccatacat tcttaatata tcttctccaa aatttgttcc tatttcttct tcactatctt
      961 caagtaagta tataaaatca caatattcat ctataattcc agaatctcca aaatcaatta
     1021 ttccagttaa tctattattg ccatctaaca atagatgatt acaactaaaa tcattatggc
     1081 ataaacactt tttaccctca aaaactgttg ttgcatttag tctttccata aaactttcta
     1141 tataatcttt ttctatatca gttaaatcat tataaatagt ttcacgcaac aatatatact
     1201 cttctaatac attttgttta ttatcaatag tacattcact aatatctgta taatctaaac
     1261 cgtgcatttg tcttaaaaaa ctggcaatat ctcgttttaa caaattttgt tcttcttctg
     1321 acatagtaga ataaatttct ggtgttaaaa aagttccttt aatttcttta taacctagta
     1381 tagataattc atcactaata tacgaatatt caatattagg aatttttaca ttagtttcta
     1441 aatttgtatt taaaaaatta tatattgctt tttcttttgc ataacctttt ttcttattag
     1501 tactaaattt tgttttaaaa atgtattcat tattaactaa atatgccaca ctatcataac
     1561 cactaccgat tatttcaata ctatctactt tgaaattatc aaagtaatgc tcaattaaat
     1621 atttcattgc cttaacattt gtggcattat catcatatct atattccatt aaataacaat
     1681 cttctttttt gccctcgtgt aattcatgtt ctggcaaatc ttcaataatt ctaaaaccag
     1741 atttttggta tgcccttatt gctcttggat tatttttatg agggtctaaa ataactgcat
     1801 tagcatttct ttcttttttc aaaaattcaa aaatcaattt aatatatctt gtaccaattc
     1861 ctttactcca ataatttggc tctcctataa attgatccat accatagact atctcatcag
     1921 tttttggata atgataatca gtatataact catcatacat tttatatatt tgtccatatc
     1981 caataggaac attgttatat tcaataatta ctctaaaaac ttcatcttcc caaggctctg
     2041 tataatgttt ttttaatgat tctaatgtat attttttatc tctaccacca taaaattcta
     2101 atactctttc atcagttaac cattttaaca tcaaaggaaa atcatcatct attaaagttc
     2161 ttatacatat ttcattttca actatattca tttatttatc acctttttca taatcatata
     2221 catatactat ttcatcttta taatcatttt taccacctaa tttttcatat acatggcaag
     2281 ctctaggatt acctttatca gttattaaaa acatttcaga acaaccaatc tctttagaat
     2341 attccttaat aaaagataat aattttgaac cataaccttt gtcttgatag ttaggtaaca
     2401 ttcctattga gtgtaaataa aacattgttt ttccatcagg tcttaaaagt gtatagcaat
     2461 atgcaaatcc tata
//
LOCUS       DACGLE010000151         2422 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0534
            SAMEA3304449-rid9560453.denovo.163, whole genome shotgun sequence.
ACCESSION   DACGLE010000151 DACGLE010000000
VERSION     DACGLE010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304449
            Sequence Read Archive: ERR985064
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2422)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2422)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702225.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702225.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 20:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,045
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 27 of 67
            Pseudo Genes (incomplete)         :: 25 of 67
            Pseudo Genes (internal stop)      :: 19 of 67
            Pseudo Genes (multiple problems)  :: 4 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2422
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304449-rid9560453.denovo.163"
                     /strain="VREN0534"
                     /db_xref="taxon:1352"
     gene            <1..290
                     /locus_tag="IXT20_002792"
     CDS             <1..290
                     /locus_tag="IXT20_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4861188.1"
                     /translation="ETQLWFKFDPSNRFIKDFYKVWNSEVFFLAIEDSLLINLYYSNK
                     NYFKIPAAKTRMKKDVYFLFDIVTDVPDARSDHRRYDYIKYTFVDPERYKD"
     gene            complement(803..876)
                     /locus_tag="IXT20_002793"
     tRNA            complement(803..876)
                     /locus_tag="IXT20_002793"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(840..842),aa:Ile,seq:cat)
     gene            complement(1065..2084)
                     /locus_tag="IXT20_002794"
     CDS             complement(1065..2084)
                     /locus_tag="IXT20_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAQ4861189.1"
                     /translation="MKKKITITAMSLLTALFLLPINGFAYTINNEFNLGANEGSSQVA
                     NNQYILLHETANETATGRNEAQYMQRSWTSAYTAYIVGDGGIVYQVGQPGYVQYGAGS
                     YANANSPVQIELQHTHDKATFEKNYKAYVELARDSAMKYGIPLTLDTPYNQPGIKSHL
                     WVTQNIWGDHTDPYGYLSEMGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGAAN
                     PTLTDGTNYAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADGIY
                     RSDVNQAYNTSGNVGYHVSFNMRNFPNKKVYVMMRATNDPEGNTKGGAQDFHDKRWYL
                     NIPKR"
     gene            complement(2095..2301)
                     /locus_tag="IXT20_002795"
     CDS             complement(2095..2301)
                     /locus_tag="IXT20_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holin"
                     /protein_id="HAQ4861190.1"
                     /translation="MNNRTFDILKWIAIVVIPALATFVGLVGKGVNWRYTDITVMIIT
                     GFGAFLGSILGVSNRTYKKISSDK"
BASE COUNT          740 a          466 c          405 g          811 t
ORIGIN      
        1 atgagacaca attatggttt aaatttgacc cttcgaatag atttatcaaa gatttttata
       61 aagtatggaa ttcagaagtt ttctttttag caatcgaaga tagcttatta atcaatctct
      121 actattctaa taagaactat ttcaaaattc ctgctgcgaa aactagaatg aagaaggatg
      181 tatacttttt gtttgatatc gtgactgacg tgccggacgc acgtagcgat catcggcgtt
      241 atgactatat aaagtatact ttcgttgatc cagaaagata caaagattaa agtaggctac
      301 ctaaaaaggt agcccggaac gaattttatc accatacttg tgaaaggaga tattttttaa
      361 gttagtatta agattgtgta atatgatgat atctatattt tatagtatca gtgctataaa
      421 atcaaaaata agtcactaat taactaccac tccaattgta agcctttttt ctcacttttt
      481 ttcaaaaata tggtatgcct tttaatagct tcaaatataa aagagtttaa agcgtaacac
      541 acttatgggg aagtggcttg gggtgcgctt taaactcttc tttattatca tctcacaatt
      601 taacccaaat gtctttctat ttaaaaatca aagtaaaact tttcaaatat acagaaatat
      661 aactatgtga aacatccttt cattaatcca taaaaggata cataaaaaag ccactcattt
      721 gagtggcaat gaagaaaagc tttagcttgt ataatactct tcaaaaaatt ctaacacaga
      781 acgattcaaa tggctacgtt aatgtaccct gtaggactcg aacctacgac cggacggtta
      841 tgagccgtct gctctgacca actgagctaa gggtactggt tgttgccaca taaagccata
      901 aacaatcaac cagtagaatg tgtggcaaca aacctgttat cgcatagctt ggagtgtgac
      961 tattatgggt gatagtgaag atatgcgata acatcactat tttatcgaat gatttttata
     1021 gttgtcaata tagttatgta atgctcctca acgaggagct atttttatcg tttaggaata
     1081 tttaaatacc aacgtttgtc atggaaatct tgcgcaccgc ctttagtgtt tccctctgga
     1141 tcattcgttg cccgcatcat gacatagact ttcttattag ggaagttacg catgttaaaa
     1201 gatacatgat agccaacatt tccagaagta ttataagctt gatttacatc tgatctataa
     1261 attccatcag ctcttactcg agctaattct ttcccagtat tgtaatccat aatgaagata
     1321 tactcgtatt tatagttagc aatgtgccat ccagctacat gcaaatttgc attttcgatt
     1381 tctccaaact gatcaatgtg ggcgtaattc gttccatctg tcagtgtagg attagctgca
     1441 ccagctcgtg ttggatcaat gacaggcttg ttttcagaag ttgttggatt ttcatcggta
     1501 aatccatgag ctaaatcata tgctaatttt tctttactta cgcccatttc agaaagataa
     1561 ccgtaaggat ctgtatgatc gccccagatg ttttgtgtta cccataaatg cgatttgatt
     1621 cccggttggt tataaggagt gtccaacgtt aatggaatac catatttcat tgctgaatct
     1681 ctagccaatt caacgtatgc cttgtagttt ttctcaaacg ttgctttatc atgtgtgtgt
     1741 tgtaactcaa tctgcacagg actgttggca ttagcatacg aaccagcacc gtactgtaca
     1801 taaccaggtt gaccgacttg ataaacaatt ccgccgtctc ccacaatata agcagtataa
     1861 gcgctagtcc atgaacgttg catatactgc gcttcattgc gtcctgttgc tgtttcatta
     1921 gccgtttcat gcagtaaaat atactgatta tttgctactt gtgagctacc ttcatttgcg
     1981 cccaaattaa attcattgtt gatagtatag gcaaatccgt taattggcaa taaaaaaaga
     2041 gccgttaaca ggctcatcgc agtaatagta attttctttt tcatatctat cctcctattt
     2101 atctgatgaa attttcttat acgttcgatt tgatacacct aaaatactcc ctagaaatgc
     2161 tccaaaacct gtaataatca tcaccgttat atctgtgtac cgccagttaa caccttttcc
     2221 gacaagaccg acaaaagtcg ctaaagcggg gataactact atagcgatcc atttcaaaat
     2281 atcaaaggtt ctattgttca tactatccac ctcccttcat tggttgggct actttctttt
     2341 tcaatcgctt gttttcttct atcaatattt ctctttctga aagcaaggca tcaatttgcc
     2401 cgtccagttg ttcaatttta tc
//
LOCUS       DACGLE010000152         2398 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0534
            SAMEA3304449-rid9560453.denovo.164, whole genome shotgun sequence.
ACCESSION   DACGLE010000152 DACGLE010000000
VERSION     DACGLE010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304449
            Sequence Read Archive: ERR985064
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2398)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2398)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702225.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702225.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 20:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,045
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 27 of 67
            Pseudo Genes (incomplete)         :: 25 of 67
            Pseudo Genes (internal stop)      :: 19 of 67
            Pseudo Genes (multiple problems)  :: 4 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2398
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304449-rid9560453.denovo.164"
                     /strain="VREN0534"
                     /db_xref="taxon:1352"
     gene            207..2069
                     /locus_tag="IXT20_002796"
     CDS             207..2069
                     /locus_tag="IXT20_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ4861191.1"
                     /translation="MQQLTKRERQLLIYLVEMSEPVTIKKLAEKENVSVRTIKYDLDD
                     IREWLNERKQVLYSKRSQGIWMNISSSDRIKLKSELMDVNRFEIFADQSLRIKRLLIM
                     LLVTKEAITGMKMANALDVSKDTIMNDLDVLENNFLKEGLTLNRQARKGFWIIGEERL
                     VRLTIEEILQKEFTDYDIYKLMSLLLNGGETEYFEMYSATATPIQEVFNQVIIRMRHL
                     LEFENLEKLNYAELLNILIRVTIATVRLRKEATIGRYQLVAEQELDKNDLSYRVMQQV
                     FDYYQLPLFEDEYHYIYSDTFDTIHQQDVMKLVENVIYKVSKAINHSFYQDPQLLTNL
                     YAHLSMRLSRKKKFVNEYNPFKEDIKQKYPELFDAIKQAVRQEISEQNILINDSFVAY
                     IALHFLVSYEREEDLRSVRAVYICSTGLGVTSLIKQKINEELNNITIVAFASVLNAQE
                     MIATKDPDLVISIFPINGIDRPFIKVHPLPTEQDLKKIQQIIKKILASSNQQERHNLN
                     LRETSINKDTLEDFSKELILKAYTIYERLLVILEAELIQEYREAFLLHVFLMVHRITF
                     DQQYMAEGRQVDQIILDKGTLVGKIEAVFAENNLGINQSEISALFSYIKEGASS"
     gene            2069..>2398
                     /locus_tag="IXT20_002797"
     CDS             2069..>2398
                     /locus_tag="IXT20_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ4861192.1"
                     /translation="MKMSDEALKIIEESQNREKLKQVIDLIEERLVEHAIVPTELQWT
                     ILINHLNEMLKRSQRKETIPVVDRDLFKEVSKEAITISDEIVRSIGNLTDDEIYVLSI
                     HFETAKNN"
BASE COUNT          865 a          353 c          466 g          714 t
ORIGIN      
        1 aaaaatttga aaaagcgttt gattatttat attacgactt agtgcaataa aaaacgcttt
       61 ttaacagtac atgtagtcgt ctttataata gataaactct gtaatattag ttaaaaagct
      121 ataagttgca cttagttaag tgcaaacatt tcatattgtc gttagtttag aaaacgctta
      181 tattagcaat gtaaggagag atgataatgc agcagctaac aaaaagagaa aggcagcttt
      241 tgatttactt ggtggaaatg tctgaaccgg taacaatcaa aaagttggct gaaaaagaaa
      301 atgttagtgt tcggacgata aaatacgatc tagatgatat aagagagtgg ctgaatgagc
      361 ggaagcaagt cctatatagc aaacgtagtc agggaatttg gatgaatatt agtagctctg
      421 atcgaatcaa attaaaaagc gaattgatgg atgttaatcg ttttgaaata tttgcagatc
      481 aaagtctccg aatcaaacgt ttactaatta tgcttttagt aacaaaagaa gctattactg
      541 gaatgaaaat ggccaacgca ttagatgtca gcaaagatac gatcatgaat gatttagatg
      601 tgctggaaaa taattttctt aaggaaggat tgaccttgaa tcgtcaggcg cgaaagggtt
      661 tttggatcat aggagaagaa cgtctagttc gattaacgat tgaagagatt cttcaaaaag
      721 agttcacgga ttatgacatt tataaattaa tgtcattgtt attgaatggc ggtgagactg
      781 aatactttga aatgtattct gctactgcta cacctatcca agaggtattt aatcaagtaa
      841 tcattcgaat gcgacactta ctggaatttg aaaatctaga gaaactgaat tacgcggaat
      901 tactaaatat tctgattcga gtgacaattg ctaccgttcg cttacgcaaa gaggctacga
      961 ttggtcgtta tcaattggtt gctgaacaag aattagacaa aaacgatctt tcttatcgcg
     1021 tcatgcagca ggtctttgat tattatcagt tgccactgtt cgaggatgaa tatcattata
     1081 tctatagtga tacattcgac acgattcacc agcaagacgt catgaaacta gttgagaatg
     1141 tgatttataa agtaagtaaa gcaatcaacc attcttttta tcaagatcca caattgttga
     1201 cgaatctgta tgctcattta tccatgcgat tatcgagaaa gaaaaaattt gtcaatgaat
     1261 ataatccttt taaagaagat atcaagcaga aatacccgga attatttgat gcgatcaagc
     1321 aagcagttcg acaagaaata agtgaacaaa atattctgat caatgattct ttcgtggcat
     1381 atattgcctt acactttttg gtttcctacg aaagagaaga agatctacga agcgttagag
     1441 ccgtctacat ttgttcaaca gggttgggtg tgactagtct gatcaagcaa aagatcaatg
     1501 aagaattaaa taatatcact atcgtggcat ttgcttcagt attgaatgcg caagaaatga
     1561 ttgctaccaa agatcctgat ttggtgatta gtatttttcc aattaatggc atcgatcgtc
     1621 ctttcatcaa agtccatcca ttacccactg aacaggactt gaaaaaaatt caacaaataa
     1681 taaaaaaaat acttgcttct tccaatcagc aggaaaggca taacttgaat cttagagaaa
     1741 cgtccatcaa taaagatact ttagaagact ttagcaagga attgattttg aaagcttata
     1801 cgatttatga acgtttactt gtcattttgg aagcagaatt gatccaagaa tatcgagaag
     1861 cttttttgct tcatgtattc ttaatggttc atcgtatcac atttgatcaa caatatatgg
     1921 cagaaggtcg tcaagtagac cagattattt tagacaaagg aacactggta ggaaaaatcg
     1981 aagcagtttt tgcagaaaat aatttaggaa tcaatcagtc agaaatcagt gcattattct
     2041 catatataaa agaaggagcg tcaagttagt gaaaatgtca gacgaggctt taaaaattat
     2101 cgaggaaagt caaaatcggg aaaagttaaa acaagtgatt gatctaattg aagagcgatt
     2161 agtagaacat gctattgtac caaccgagtt gcagtggacc attctaatta atcacttgaa
     2221 tgagatgctg aaacgttctc aaagaaagga aacgattccc gtagtcgatc gggatttgtt
     2281 caaagaagta tccaaagaag ccataactat ttctgatgaa atagttagaa gtatcggtaa
     2341 tttaaccgat gatgaaatat atgttttgtc gattcatttt gaaacagcaa aaaataat
//
LOCUS       DACGLE010000153         2369 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0534
            SAMEA3304449-rid9560453.denovo.165, whole genome shotgun sequence.
ACCESSION   DACGLE010000153 DACGLE010000000
VERSION     DACGLE010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304449
            Sequence Read Archive: ERR985064
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2369)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2369)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702225.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702225.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 20:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,045
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 27 of 67
            Pseudo Genes (incomplete)         :: 25 of 67
            Pseudo Genes (internal stop)      :: 19 of 67
            Pseudo Genes (multiple problems)  :: 4 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2369
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304449-rid9560453.denovo.165"
                     /strain="VREN0534"
                     /db_xref="taxon:1352"
     gene            221..655
                     /locus_tag="IXT20_002798"
     CDS             221..655
                     /locus_tag="IXT20_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HAQ4861193.1"
                     /translation="MSKRYQNIMVAVDGSRQSIKAFAEALDLAKDNEAHLFIVSIINK
                     VELTHSAYAFSKIYADEKQRTEVAMLKKINDAKEYGIAEIHAIVETGDPRNLIANVIP
                     GQENIDLIVMGATGKGAIQQALVGSTASYVVSHAPCSVLVVK"
     gene            678..998
                     /locus_tag="IXT20_002799"
     CDS             678..998
                     /locus_tag="IXT20_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4861194.1"
                     /translation="MVRPQEVRAPKEKIEILAIIEDGTQTKKGYSIALVKWKGKKGVA
                     LRWDGDNQQDKGFPVTANGYHPAWFVLPDKFMELYSYDYARAAASIKALDKLTEEQNE
                     MEEK"
     gene            1139..1330
                     /locus_tag="IXT20_002800"
     CDS             1139..1330
                     /locus_tag="IXT20_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4861195.1"
                     /translation="MEAGKVLQEVKALCADKKYEEAKSLIEKNKENLEEKYPIAQEFI
                     ELKQGTILHRFKHFFGITE"
     gene            complement(1401..1658)
                     /locus_tag="IXT20_002801"
     CDS             complement(1401..1658)
                     /locus_tag="IXT20_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake protein"
                     /protein_id="HAQ4861196.1"
                     /translation="MIAIGQFVFYIPFFIMISILFYYIKWTKKKFSVLLASLPAVYFT
                     YQIFSFRHWETTSVLITHIIELTLSVIFLIIWIYFLYKNQN"
     gene            1816..2079
                     /locus_tag="IXT20_002802"
     CDS             1816..2079
                     /locus_tag="IXT20_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4861197.1"
                     /translation="MDHVVLEIDLHLINNIRVIYYVVSNSVEQRVLTNKINGILAKKD
                     VHRFNNGEGSYYSIPVEKIIYTTVKVREDLDTKKAYEPIFTTY"
     gene            2103..2291
                     /locus_tag="IXT20_002803"
     CDS             2103..2291
                     /locus_tag="IXT20_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4861198.1"
                     /translation="MNPYEQLIQDLIDGKIEKIDVKREELMAFREAWLKLENRKYVVG
                     KASLNGNVTYHYDPTRLF"
BASE COUNT          921 a          331 c          491 g          626 t
ORIGIN      
        1 cgttttttgt aacagaatga tgttcgctcg atgtgaaaaa tgatgaggaa tttcacataa
       61 tgcagaaata atgggattag aacaggtgtt ctgatgagaa aagaaatgta taaataactt
      121 gaaagaaatt gcttttgttc atcattttat ttacatattg cgtatatttt ttgtattttt
      181 gcttgagatg ttacaataaa agaaaaagga ggcggttcca atgagtaaaa gataccagaa
      241 catcatggta gcagttgacg gatcaagaca atcgatcaaa gcgttcgctg aagcccttga
      301 cttagcaaag gacaatgaag cccacttatt tatcgtgtca atcatcaaca aagttgagct
      361 gacccatagt gcctatgcct tttcaaaaat ctatgcagat gaaaaacaaa gaacagaagt
      421 agccatgctg aaaaaaatca atgatgccaa agagtacggc attgctgaga tccacgcaat
      481 agttgaaact ggagatccaa gaaatttgat tgccaatgtc atccctggac aagaaaatat
      541 tgatttgatc gttatgggag cgacaggaaa aggtgctata caacaagcat tagttggatc
      601 gaccgcttct tatgtcgtat cacatgcccc ttgcagtgtg ttggtcgtga agtaatgaga
      661 taagggagga atagaaaatg gttcgtcctc aagaagtccg tgcgccaaaa gaaaaaatag
      721 aaattttagc aattattgaa gacggcacac aaacaaagaa gggatactcg atcgctttag
      781 taaagtggaa aggaaaaaaa ggtgttgctc tgagatggga tggagataac caacaagata
      841 aaggattccc tgtcacagct aacggttatc atccagcgtg gtttgttctg cctgataaat
      901 ttatggaatt atacagctat gattatgcaa gagccgcagc atcaatcaaa gcattagata
      961 aattaacaga agagcagaat gaaatggagg aaaaataaaa aagacgaacc aatcagcctt
     1021 tagaaaactg attagttcga ggagtaaaaa tgaaaaaagg ttatttggga tgaaactaga
     1081 ataatgcgta taacttaaat aaaacttaaa gcaataaaat ggcaagaggg tgaggcaaat
     1141 ggaagcagga aaagtattac aggaagtaaa agcactatgt gcagataaaa aatatgaaga
     1201 agcgaaaagc ctgatcgaaa agaacaaaga aaatttggaa gaaaagtacc cgatcgcaca
     1261 agaatttata gaattaaaac aaggaacgat ccttcaccga ttcaaacatt tttttggtat
     1321 aacagaatag aaaaaacgat taaagatcgg cagaaatttg ctgcatcttt aatcgttttc
     1381 tttttaaaca tgcgagggat tcaattctga ttcttatata aaaaatagat ccaaataata
     1441 agaaaaatta ctgaaagcgt caattcgatg atgtgtgtta ttaatacaga cgttgtttcc
     1501 caatgcctaa aagaaaaaat ttgataagta aaatatacag caggtaaaga ggccagtaaa
     1561 actgaaaact tcttttttgt ccattttatg taatagaaaa gaatgcttat cataataaaa
     1621 aaaggaatat aaaaaacaaa ttgaccgatc gcgatcatac tattgcctcc gataaataaa
     1681 gtccatgata caaaataaca ctttgtgcga aatggcgcaa gcagttttgt ggatttatcc
     1741 ggtaaaatct gtatcgattt tgaaattaga ggagaaactt gctataatca aagtgaaaga
     1801 aggagtggga ttttcatgga tcatgtagtg ttagaaatcg atttgcattt gatcaataat
     1861 attcgggtga tttactatgt tgtaagtaac agtgtcgaac aacgtgtact gacgaataaa
     1921 ataaatggta ttttagcaaa gaaagacgtt caccgattca ataatggtga aggaagttat
     1981 tatagtatcc cagtagaaaa aattatttat acgacagtca aagtcagaga agatctagat
     2041 accaaaaaag catatgaacc tatttttaca acttattgat tgacagcaga tggaggaaaa
     2101 aaatgaatcc atatgaacaa cttattcaag atttgatcga tggaaaaatc gaaaagattg
     2161 atgtaaaaag agaagaatta atggcttttc gagaagcgtg gctgaagttg gagaatcgta
     2221 aatatgtggt gggaaaagcc tcactgaacg gcaatgtgac ttatcattac gatccgacac
     2281 gtttatttta gtatgaagta ttcagaaaag aacggctctt tgtcaaatag gactgataga
     2341 gtgagataag aacatctaag gtagaattc
//
LOCUS       DACGLE010000154         2291 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0534
            SAMEA3304449-rid9560453.denovo.166, whole genome shotgun sequence.
ACCESSION   DACGLE010000154 DACGLE010000000
VERSION     DACGLE010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304449
            Sequence Read Archive: ERR985064
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2291)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2291)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702225.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702225.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 20:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,045
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 27 of 67
            Pseudo Genes (incomplete)         :: 25 of 67
            Pseudo Genes (internal stop)      :: 19 of 67
            Pseudo Genes (multiple problems)  :: 4 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2291
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304449-rid9560453.denovo.166"
                     /strain="VREN0534"
                     /db_xref="taxon:1352"
     gene            complement(36..1355)
                     /locus_tag="IXT20_002804"
     CDS             complement(36..1355)
                     /locus_tag="IXT20_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAQ4861199.1"
                     /translation="MKKLKIFPKMFIQIFSVLGIIIILVHSLIFFIFPKTYLETRKEK
                     IHNMANEISSNMNGKEIKYIEQTLEFYSKSSEIKVFIKEKNNKNEIQIKDNINVNLES
                     DSNSLIIEEREIKLNDGKKTNLQFVSTADMQKDAKDLSLKFLPYSLLISILFSAIISL
                     IYAKSIKNNIQEIKIVTDKMMKLDKKMSLKVSSNDEVGELKQQINDLYSTLLRTIDDL
                     EFKNKEILKLEKLKYDFFKGASHELKTPLASLKIILENMKYNIGKYKERDIYINECIE
                     IVDSLTKNISQILSVHSIENLNNDEEYLKINDTLEDILKKYEILANQKKITVNNYILD
                     ENVYIGKTALKIILSNLISNAVKYTDVNGVINIGIVNDWLYIENSYGNNKISNMDKIF
                     DVKFDLNKENSNGLGLYIVSNILNNYNIEYKVLQDEEFFIFKIKIFS"
     gene            complement(1352..2005)
                     /locus_tag="IXT20_002805"
     CDS             complement(1352..2005)
                     /locus_tag="IXT20_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ4861200.1"
                     /translation="MKILTVEDDNMIREGISEYLSEFGYTVIQAKDGREALSKFNSDI
                     NLVILDIQIPFINGLEVLKEIRKKSNLPILILTAFSDEEYKIDAFTNLVDGYVEKPFS
                     LPVLKARIDSLIKKNYGHLEKFEYKDLSVNFNSYTAKINGEEIDVNAKELEVLKCLLD
                     NNGQVLTRMQIIDYVWKDSEETPYDRVIDVYIKELRKKLQLDCITTIRNVGYKLERK"
BASE COUNT          756 a          357 c          195 g          983 t
ORIGIN      
        1 tatttttcaa taactattgc aaaattacat cttaattaag aaaatatttt tattttaaag
       61 ataaaaaatt cttcatcctg caatacttta tattctatat tgtaattatt cagaatatta
      121 cttacaatat ataaacctaa tccattacta ttttccttat ttaaatcaaa tttaacatca
      181 aatattttat ccatattcga aattttattg ttaccgtatg aattctctat atataaccaa
      241 tcattaacta tcccaatatt aattacccca tttacatcag tatattttac cgcattacta
      301 atcaaattag atagaataat ctttaaagct gtttttccta tataaacatt ctcgtctaat
      361 atataattat tgacagttat tttcttttga tttgctaata tttcatactt ttttaatatg
      421 tcttctaatg tatcatttat tttcaaatat tcttcatcat tattcaaatt ttctatagaa
      481 tgaactgata atatttgaga aatatttttt gttaaactat caacgatttc aatacattca
      541 ttaatataaa tatctctttc tttatatttt ccaatattgt atttcatgtt ttccagtatt
      601 attttaagac tagcaagagg ggttttaagt tcatgggatg cacctttaaa aaagtcatat
      661 tttaactttt ctaattttaa aatttcttta tttttaaatt ccaaatcgtc aattgttctt
      721 aataaagtag aatacaaatc attaatttgt tgttttaatt ctccaacttc atcattagag
      781 ctaactttta aactcatttt tttatcaagt ttcatcattt tatcagtaac aatttttatt
      841 tcttgtatat tattttttat tgattttgca taaattaaag aaataatagc agaaaataaa
      901 attgatatta ataaagaata tggtaaaaat ttaaggctta aatctttcgc atctttttgc
      961 atatcagctg tagaaacaaa ttgtagattt gtttttttac catcattaag ctttatttct
     1021 ctttcttcaa ttatcaaaga attactatca ctttctaaat taacatttat attatctttg
     1081 atttgtattt cattcttatt atttttttct tttatgaata cttttatttc actactcttg
     1141 gaataaaact ccaaagtttg ttctatatat tttatttctt ttccattcat attactagaa
     1201 atttcatttg ccatattatg aatcttttct tttctcgtct ccaaatatgt tttaggaaag
     1261 ataaaaaaaa ttaatgaatg aactaatata attattattc caagaacaga aaatatttgt
     1321 atgaacattt ttggaaatat ctttaatttt ttcattttct ctccaattta tatcctacat
     1381 ttcttatagt agttatacag tctaattgta atttttttct aagttctttt atatatacat
     1441 ctattactcg atcataagga gtttcttcgc tatctttcca tacataatca ataatttgca
     1501 ttcttgttaa cacttgtcca ttattatcca ataaacattt taatacttct agttcttttg
     1561 catttacatc tatttcttca ccatttattt tagctgtata gctattaaaa ttaaccgata
     1621 gatccttata ttcaaacttc tctaaatgtc cataattctt cttaattaaa gaatctattc
     1681 tagcttttaa gaccggcaat gaaaatggct tttctacata tccgtctact aaattagtaa
     1741 atgcatcaat tttatattct tcatcactaa atgcagtcaa gattagaatt ggtaaattgc
     1801 ttttctttct aatttctttc aacacttcta aaccatttat aaaaggtatc tgaatgtcta
     1861 agataaccaa atttatatcg ctattaaatt ttgacaaggc ttctcttcca tctttagctt
     1921 gaataacagt atatccaaat tctgaaagat attcacttat tccctctctt atcatattat
     1981 catcttcaac agttaatatt ttcatatata cctccactta agcctataca ctagatatta
     2041 tacagcatat tatggctcta taatatctag tgttggtgac gccttctgtt gccaatactt
     2101 tttctttttt ttaataaatg caaacagaca ccttgaaatc ctttagaata aagggttcta
     2161 taatattttg tgttgttgga aaatcgaagg cgattttcta ctaacacttt ttcttcgttt
     2221 caactcaaaa agagactcac ttttggtaaa atttagtcga ccaaaacatc accctaccaa
     2281 aggagtcgct c
//
LOCUS       DACGLE010000155         2289 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0534
            SAMEA3304449-rid9560453.denovo.167, whole genome shotgun sequence.
ACCESSION   DACGLE010000155 DACGLE010000000
VERSION     DACGLE010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304449
            Sequence Read Archive: ERR985064
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2289)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2289)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702225.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702225.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 20:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,045
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 27 of 67
            Pseudo Genes (incomplete)         :: 25 of 67
            Pseudo Genes (internal stop)      :: 19 of 67
            Pseudo Genes (multiple problems)  :: 4 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2289
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304449-rid9560453.denovo.167"
                     /strain="VREN0534"
                     /db_xref="taxon:1352"
     gene            complement(62..1444)
                     /locus_tag="IXT20_002806"
                     /pseudo
     CDS             complement(62..1444)
                     /locus_tag="IXT20_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010726506.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            1906..2187
                     /locus_tag="IXT20_002807"
                     /pseudo
     CDS             1906..2187
                     /locus_tag="IXT20_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295604.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          689 a          382 c          385 g          833 t
ORIGIN      
        1 acttgtattg tgcaatagtt ttttacaaaa aaaaggggtg cggatatttt tttggacctt
       61 tttaacaaat atatctttgc ttcttccgat attgaagagg gcttagccct cctaaagata
      121 acttaattcg ttgattatta taccaatgaa tataattatc caatttgttt ataaactgat
      181 tgatcgttgt attcttccag tcacgttgat aaaacatctc gttttttaat cgtccaaaga
      241 aaccttcgca cgctgaatta tctggtgaat aacccttttt agacatagag tgaagcaagt
      301 tggctttgtt ccaatagtcc aactaacaat agcaccatcg taacaatcga caagtggtga
      361 taagtagatt ttccctgcga gaattctaaa ttcagtaata tctgttaacc atttttcatt
      421 tggcttcttt gctttaaaat tccgatttaa taaattgggg tgtgcaggag aaatttcccc
      481 agcatacgaa gaatactttt ttcgtctaga aaatttaaca aatagatttt cctcagtcat
      541 caatctacgt acaacttttt cagaaagctt tttacccata ttttttaaag cttggtgaat
      601 tctacggtaa ccatacgcac agaagttatg attgaaaatc gtgataatta atttgacgtt
      661 ctttgtaata cttatctggt ttctcaagtg cttgtttttg ataaaaatag ctgcttttag
      721 gaaactggag ctgttgaaga agctctttca aggtgaactg atttcgtaag gcatcaatca
      781 atatcgtttt ttcttgattt gatagacaaa gaagattgac gcctttttct ttttttagca
      841 gttcagccgc tccttcgagt aatgcttttt cgagctgtaa ttggtgtact tgtttttgta
      901 agtcttctac ttcctttaaa agtagctctt tttgtttttc ctgatttttt aaagtcatac
      961 acaatggaaa atcctttcca agaaccgtgt ttttccattg atatagagta actctactaa
     1021 ttcctccaga ttcggctaaa tttttcctat tagtttgcgg tttatagaat tttttcaaca
     1081 cagcttcttt ttgatcttgt gttaacttta ccgcactttt tcggagtttt ctagctcctg
     1141 gtgcaagttc ttcacaccgc tgccgcaagg actctctatt aggataacca agcatgcgaa
     1201 tggttcgtgc ataacattga ccatattcaa aataatgatt caccgctatt cgtttttgtt
     1261 cttcagaata ctttgatttt tttcggtgct cctgatggag ctctccggat tccaaatact
     1321 ctttatacca tttccgtagt gcaccaatcg acggataccc taattctcga atggttgcag
     1381 cataagagca atcgtatttt aaaaataact gaattgccct tacacgctct ttgtgggaaa
     1441 acacacggac attcctccta agtttatttt ttcgtccagg attatgtccg cacccccttg
     1501 ctttctgatg acttattctg gttctttgag tgtcaatgca tagatgtgat gtagctagtt
     1561 agaaaaaaat tttgaatact ctttttttct ttcagactcc tttttaagta agattttgaa
     1621 gtgtgagtta cggggtcaga aacttaaaag tttttgatcc tttattttca agtctgattt
     1681 ggcagctttt cggcagctca tttatttatg gtcagaaata gatgctgaaa ttttaatcta
     1741 ttagcaaata gtccagaacg tatgaagagg agtcggatat catttgtcat taacaccata
     1801 aatatttata aaattcgtgt tcacctacaa ttaaatttaa tatataatat tcgttgtaag
     1861 ggcttataat tttttgttaa tgaacaagat aggaagaaac atttaatgga cagttttcag
     1921 agattgtcac tagtaaatca atttgagtta ttggctaaat ttgaggacca aaacagatct
     1981 tattatgaaa gaaaaatcga aatattgaga gaaggttacg agtatcatta tgatgatgaa
     2041 atttggagtg atctttctga gccatttccg aaagaagatt ctcgatttgt attagatgta
     2101 ttgaacatgt atcgagatat caattttagt aaatcaaagc taaacttaga tgaaaaccaa
     2161 attactaatt catactatac tcatttcggt tctgttgcaa agttttccaa aaaatctatt
     2221 ttagtgtaaa attgagaaaa aagacagaga ggacagagta atgaatcatt ttaaaggcaa
     2281 acaattcaa
//
LOCUS       DACGLE010000156         1900 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0534
            SAMEA3304449-rid9560453.denovo.168, whole genome shotgun sequence.
ACCESSION   DACGLE010000156 DACGLE010000000
VERSION     DACGLE010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304449
            Sequence Read Archive: ERR985064
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1900)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1900)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702225.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702225.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 20:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,045
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 27 of 67
            Pseudo Genes (incomplete)         :: 25 of 67
            Pseudo Genes (internal stop)      :: 19 of 67
            Pseudo Genes (multiple problems)  :: 4 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1900
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304449-rid9560453.denovo.168"
                     /strain="VREN0534"
                     /db_xref="taxon:1352"
     gene            <1..512
                     /locus_tag="IXT20_002808"
     CDS             <1..512
                     /locus_tag="IXT20_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="haloacid dehalogenase-like hydrolase"
                     /protein_id="HAQ4861201.1"
                     /translation="ICLTGNPGTGVSNRTIEKAITLSKSIFNGLIIAGKMHGAGVDEP
                     IMNLDIAKRFIKTGVDILLVPAPYTVPYFMESDLRGIVDYIKDFNRNKSIEEKVLVLT
                     ANGTSQDSSDQETIKKIALASKACGADIQHIGDSLNGICLPENIFALGQAIRGYRHQI
                     IMLGKSNYR"
     gene            531..1781
                     /locus_tag="IXT20_002809"
     CDS             531..1781
                     /locus_tag="IXT20_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ4861202.1"
                     /translation="MIDKVQKIAAKISNQRHLLALRDSMAAVMPLIIIGSFFMLLANF
                     PIEGFTKWLSEIGVQSFLNKASDSTFGIIGLAVTYSLGHNLAKTYGVDSLSAGLLSLS
                     GYVLLTPLLDADGSIGFPTKYFGTSGLFVGIIVALVSTEIFRWFVLKNITIKMPASVP
                     PNVSRAFVAIIPGFFVVLLWFIVVVICYKLGIENVHALIADTLAKPLSLLTKTLPGII
                     LVILIQCFFWMFGIHGAQVTGPIIEPLLLQNSDANRIAYQAGKELPNIITYEFLYNFV
                     FSGGAGCLFALAILTFFFSKSQENKTLGKLSIAPVSFQVAEPLLFGFPTILNIKMLIP
                     FVTAPVVTTLITYFSMSMGLVAKPVGATIPWTTPPIIAGFLASGGRISGAVIQVITIA
                     INVLIYYPFFKLDDNAKLKSEKND"
BASE COUNT          622 a          282 c          335 g          661 t
ORIGIN      
        1 ttatttgttt aactggaaat ccaggaacag gagtatctaa tagaactatt gagaaagcaa
       61 taacattatc caaatcaatt tttaatggat taattatcgc tggaaaaatg catggtgctg
      121 gtgttgatga accaattatg aacttagata tagcaaaaag atttattaaa acaggtgtag
      181 atattttatt agttcctgct ccatacactg ttccgtattt catggaaagt gatttgcgag
      241 gaatcgttga ttatataaaa gattttaata gaaataaatc tattgaggaa aaagttctag
      301 tactaacggc taatggaaca agccaagact cttctgacca agaaactatt aaaaaaattg
      361 cattagcatc aaaagcatgt ggagctgata ttcaacacat aggggattct ttaaatggta
      421 tttgtcttcc agaaaatatt tttgctttgg gtcaagcaat tcggggatat cgtcatcaaa
      481 ttattatgtt aggaaaatct aattatcgtt aattaaggag gaaatgaaag atgatcgaca
      541 aagttcaaaa aatagctgca aaaattagta atcaaagaca tctactagct ttaagagaca
      601 gcatggctgc agttatgcca ttaatcatta ttggttcctt ttttatgtta ttagcaaatt
      661 tcccgataga aggatttaca aaatggttat ctgaaattgg agtacaatcc tttttgaaca
      721 aagcatcaga ttcaacattt ggtattattg gattagctgt tacctactct ttgggacaca
      781 atctagcaaa gacttatggt gtagactcat tatcagcagg attattatct ttgagtggat
      841 atgttttact tacaccttta ttggatgcag atggttctat aggatttcca acaaaatact
      901 ttggtacctc gggtcttttt gttggtatta ttgtagcact tgtatctaca gaaattttcc
      961 ggtggtttgt acttaaaaat attacaatta aaatgccggc aagcgttcca ccaaatgtaa
     1021 gccgagcttt cgttgctatt attcctggct tttttgtcgt tctactatgg tttattgttg
     1081 ttgtaatttg ttataaactt ggaattgaaa atgttcatgc tcttattgca gatacactcg
     1141 caaaaccact atctttactg acgaaaactt taccaggaat tatattggtt atacttattc
     1201 aatgtttctt ttggatgttt ggtattcatg gtgcacaggt aacggggcca ataattgaac
     1261 cgttattatt gcaaaactct gatgcaaata gaattgctta tcaagctgga aaagaacttc
     1321 ctaacattat aacttatgaa ttcttatata actttgtttt ttctggtggt gcaggttgtt
     1381 tatttgcact tgccatttta acatttttct tttcaaagag tcaggaaaat aaaactttag
     1441 gtaagttatc aatcgctcca gtaagttttc aagtagcaga gccccttcta tttgggtttc
     1501 caactatatt aaatataaaa atgttaattc catttgtaac tgcaccagta gtaacaacat
     1561 taattactta tttttcaatg agtatgggat tagttgcaaa acctgtagga gcaacaatcc
     1621 catggacaac tccaccgata attgccggat ttttagcttc tggtggacga ataagtggtg
     1681 cagtgattca agtaataaca attgcaataa atgttttgat atactatcca ttctttaaat
     1741 tagatgataa tgccaagcta aaaagcgaaa aaaatgatta gaactaccta tatgtttcta
     1801 actgtagtta agtatgaaaa gactattttt aaatttagca tttttgcgct atcggcgtag
     1861 atgaagtatc acgataacga aattagtttc tttcatttag
//
LOCUS       DACGLE010000157         1843 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0534
            SAMEA3304449-rid9560453.denovo.169, whole genome shotgun sequence.
ACCESSION   DACGLE010000157 DACGLE010000000
VERSION     DACGLE010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304449
            Sequence Read Archive: ERR985064
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1843)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1843)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702225.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702225.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 20:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,045
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 27 of 67
            Pseudo Genes (incomplete)         :: 25 of 67
            Pseudo Genes (internal stop)      :: 19 of 67
            Pseudo Genes (multiple problems)  :: 4 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1843
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304449-rid9560453.denovo.169"
                     /strain="VREN0534"
                     /db_xref="taxon:1352"
     gene            complement(<1..612)
                     /locus_tag="IXT20_002810"
     CDS             complement(<1..612)
                     /locus_tag="IXT20_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4861203.1"
                     /translation="MTETKFIQDPWSKMTTRNGLRGDEAISALQKSIRRGKEKEACEF
                     AYEMYITSPQFEEKLWRRLLAISVEDVGMGNPMAAIMINNLNAMRKEFQYNESDRAMY
                     FFHAIRYLCQSEKDRSSDLLKNIVIKSFAMGYIPEIPDIALDKHTVRGQAMGRGSEHF
                     LHEASKVIPQAKVDNDYKEQYEKIIETYDPDHVVPSTFSFNPWQ"
     gene            653..1345
                     /locus_tag="IXT20_002811"
     CDS             653..1345
                     /locus_tag="IXT20_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005470391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAQ4861204.1"
                     /translation="MDVEMLIDKYQLNDSEAEVLRFMKKNRQSLKNIGIRDVAKASYV
                     STATIINMAKKIGYSGYSELVFALGNSQSDITMPSTFSNEEKEAFIHLMSAYRDKRIM
                     ILGSGFSQNLANYFSESLNLYGFRATANSHLEFLRQQVQQDILLFIISNSGDTVRLAE
                     LAKIANDHFIDVIAFVGQKNSKIGTLATLTISTETYIPQKISDYQPNLFFGTTLNQFE
                     LLLSAALKAIYK"
BASE COUNT          563 a          346 c          329 g          605 t
ORIGIN      
        1 ttgccaagga ttaaatgaaa acgtacttgg cacgacgtga tctggatcat aggtctcgat
       61 gattttttcg tattgttcct tgtagtcatt atcaactttt gcttggggga tcactttact
      121 tgcttcatgc aaaaagtgtt ctgaaccacg ccccattgcc tgtccccgaa cagtatgttt
      181 atccaacgca atatcaggaa tttctggaat atatcccata gcgaaactct tgataacaat
      241 gttcttaagt aaatccgatg aacgatcttt ttcagattgg cataggtaac gaatcgcgtg
      301 gaaaaaatac atcgcacgat cggattcatt gtattggaat tctttgcgca tggcgtttaa
      361 gttattgatc atgatggcgg ccataggatt gcccatgccc acatcctcaa cactgatcgc
      421 caatagcctt ctccagagtt tctcttcaaa ttgtggtgag gtgatataca tttcataagc
      481 aaattcacat gcttcttttt cttttcctcg acgaatcgat ttctgtaatg ctgagatagc
      541 ttcatctcca cgtaagccgt tacgggtagt catttttgac cacggatctt gaataaattt
      601 cgtttctgtc ataatataat actcctatta gataaaattt gaggtgaatc ttatggatgt
      661 tgaaatgctt atagataaat accaactgaa tgactcagaa gctgaagtct tacgatttat
      721 gaaaaaaaat cgtcagtctt taaaaaatat tgggattcgt gacgttgcca aagcatcgta
      781 tgtttctact gcaacgatta tcaatatggc gaagaaaatt ggatattccg gttatagtga
      841 gttggtattt gctctaggaa acagccaatc tgacattacg atgccaagca cattctccaa
      901 tgaggagaag gaagcattca tacatttaat gtctgcgtat cgcgataaac ggattatgat
      961 tcttggctca ggtttttcgc aaaatctagc caattatttt tcggaatctc ttaatctata
     1021 tggatttcgt gcgacggcca atagtcattt agaatttctt agacagcaag tgcaacaaga
     1081 tattctgctt tttattattt ccaattctgg tgatacagtt cgattagccg aattagctaa
     1141 aattgcgaac gatcacttta tcgatgtgat cgcatttgtt gggcaaaaaa attcaaaaat
     1201 cggtacgctg gcaacattaa ccattagcac ggaaacctat attccacaaa aaatatctga
     1261 ttaccaacca aatcttttct ttggcacgac actgaatcaa tttgagcttt tgttgagcgc
     1321 ggcgctgaag gctatttata aatagaagaa cgctgtggcc actgtttctt caaaatctag
     1381 tataaagaat cttctttaga atgtaattgt tttctgtgtg ttctgaacat gtttaggaat
     1441 ttccaaactt gtttagaaaa ctttctcatt ccctccgctg tagaattgaa ctcacataaa
     1501 aaacatctaa accaaaatat ctacaagctt ggaaaggata ataggttgtt attaaaaata
     1561 atttttattc ctgaacctga ataattcata gctttaattc tttggcacat tttattgaaa
     1621 tatgtatcac taatatccct atcaactgat tttgactaaa aagttacttc aaacattcac
     1681 tgttcaaaaa tattgaggaa taaattttgg gtgtaaggca ttttgagcat acttctccct
     1741 ggataacttc ccagtaaaaa aatcgttaga ttgttggatt agatccattg actgcctcgc
     1801 ctcaggcgag caaagaccct gtagaattca ttctgataca cat
//
LOCUS       DACGLE010000158         1655 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0534
            SAMEA3304449-rid9560453.denovo.170, whole genome shotgun sequence.
ACCESSION   DACGLE010000158 DACGLE010000000
VERSION     DACGLE010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304449
            Sequence Read Archive: ERR985064
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1655)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1655)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702225.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702225.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 20:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,045
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 27 of 67
            Pseudo Genes (incomplete)         :: 25 of 67
            Pseudo Genes (internal stop)      :: 19 of 67
            Pseudo Genes (multiple problems)  :: 4 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1655
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304449-rid9560453.denovo.170"
                     /strain="VREN0534"
                     /db_xref="taxon:1352"
     gene            78..193
                     /gene="rrf"
                     /locus_tag="IXT20_002812"
     rRNA            78..193
                     /gene="rrf"
                     /locus_tag="IXT20_002812"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            203..276
                     /locus_tag="IXT20_002813"
     tRNA            203..276
                     /locus_tag="IXT20_002813"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:237..239,aa:Asn,seq:gtt)
     gene            316..405
                     /locus_tag="IXT20_002814"
     tRNA            316..405
                     /locus_tag="IXT20_002814"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:350..352,aa:Ser,seq:gga)
     gene            420..491
                     /locus_tag="IXT20_002815"
     tRNA            420..491
                     /locus_tag="IXT20_002815"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:453..455,aa:Glu,seq:ttc)
     gene            507..579
                     /locus_tag="IXT20_002816"
     tRNA            507..579
                     /locus_tag="IXT20_002816"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:540..542,aa:Val,seq:tac)
     gene            589..661
                     /locus_tag="IXT20_002817"
     tRNA            589..661
                     /locus_tag="IXT20_002817"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:622..624,aa:Asp,seq:gtc)
     gene            697..772
                     /locus_tag="IXT20_002818"
     tRNA            697..772
                     /locus_tag="IXT20_002818"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:730..732,aa:Phe,seq:gaa)
     gene            774..854
                     /locus_tag="IXT20_002819"
     tRNA            774..854
                     /locus_tag="IXT20_002819"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:808..810,aa:Tyr,seq:gta)
     gene            861..934
                     /locus_tag="IXT20_002820"
     tRNA            861..934
                     /locus_tag="IXT20_002820"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:893..895,aa:Trp,seq:cca)
     gene            937..1009
                     /locus_tag="IXT20_002821"
     tRNA            937..1009
                     /locus_tag="IXT20_002821"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:970..972,aa:His,seq:gtg)
     gene            1021..1092
                     /locus_tag="IXT20_002822"
     tRNA            1021..1092
                     /locus_tag="IXT20_002822"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1053..1055,aa:Gln,seq:ttg)
     gene            1138..1208
                     /locus_tag="IXT20_002823"
     tRNA            1138..1208
                     /locus_tag="IXT20_002823"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1170..1172,aa:Cys,seq:gca)
     gene            1229..1313
                     /locus_tag="IXT20_002824"
     tRNA            1229..1313
                     /locus_tag="IXT20_002824"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1263..1265,aa:Leu,seq:caa)
     gene            1375..1447
                     /locus_tag="IXT20_002825"
     tRNA            1375..1447
                     /locus_tag="IXT20_002825"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1408..1410,aa:His,seq:gtg)
     gene            1457..1528
                     /locus_tag="IXT20_002826"
     tRNA            1457..1528
                     /locus_tag="IXT20_002826"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1489..1491,aa:Gln,seq:ttg)
     gene            1546..1630
                     /locus_tag="IXT20_002827"
     tRNA            1546..1630
                     /locus_tag="IXT20_002827"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1580..1582,aa:Leu,seq:caa)
BASE COUNT          388 a          375 c          432 g          460 t
ORIGIN      
        1 actcggaaaa aaacttatgt gacttcaaat ccagttttga gtgaacaaaa tttactcaat
       61 aaaagataac accacagtgt ggtggcgata gcgagaagga tacacctgta accatgccga
      121 acacagaagt taagcttctt agcgccgatt gtagtgaggg gttgcccctt gtgagagtag
      181 gacgtcgcca cgctgatgtt attccggcat agctcagttg gtagtagcgc atgactgtta
      241 atcatgatgt cgtaggttcg agtcctactg ccggagttct cattttgaga aagagtattg
      301 agcttctttc actctggaga gttgtccgag aggccgaagg agcatgattg gaaatcatgt
      361 agatggtgcc aaactgtctc aagggttcga atcccttact ctccgtagca aatttacctg
      421 gcccgttggt caagcggtta agacaccgcc ctttcacggc ggtaacacgg gttcgaatcc
      481 cgtacgggtc attttaattg aatgttggag gtttagctca gctgggagag catctgcctt
      541 acaagcagag ggtcagcggt tcgatcccgt taacctccat aatgaactgg ttccgtggtg
      601 taggggttaa catgcctgcc tgtcacgcag gagatcgcgg gttcgattcc cgtcggaacc
      661 gtcgttcttg ttattcaatt aataatttta aaatatggcg cggtagctca gttggtagag
      721 caacggattg aagctccgtg tgtcggcagt tcgattctgt cccgcgccac catggaggag
      781 tagcgaagtg gctaaacgcg acggactgta aatccgttcc ttagggttca gtggttcgaa
      841 tccactctcc tccattttat aggggcatag tttaaaggta gaacagcggt ctccaaaacc
      901 gttagtgtgg gttcaattcc tgctgcccct gccatggcga atgtggcgaa gtggttaacg
      961 caccggattg tggctccggc attcgtgggt tcgattccca tcattcgccc tttttattct
     1021 tggggtatag ccaagcggta aggcaaggga ctttgactcc ctcatgcgtt ggttcgaatc
     1081 cagctacccc agtttctata atttattctt tattttatag attgataata gtataatggc
     1141 ggtatagcca agtggtaagg cagaggtctg caaaaccttc atcaccggtt caaatccggt
     1201 taccgcctta gtactaataa atcaacttgc cggcgtggcg gaattggcag acgcgctgga
     1261 ctcaaaatcc agtgcccgtt gagggcgtgc cggttcgacc ccggccgccg gtattgacta
     1321 agatgtcttc atcttagttt tttattgaga aattaaataa catcttttat gatggcgaat
     1381 gtggcgaagt ggttaacgca ccggattgtg gctccggcat tcgtgggttc gattcccatc
     1441 attcgccctt tattcttggg gtatagccaa gcggtaaggc aagggacttt gactccctca
     1501 tgcgttggtt cgaatccagc taccccagtt attattttta aatttgccgg cgtggcggaa
     1561 ttggcagacg cgctggactc aaaatccagt gtccgttgag gacgtgccgg ttcgaccccg
     1621 gccgccggta taattatcaa tatagcaact ttctt
//
LOCUS       DACGLE010000159         1605 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0534
            SAMEA3304449-rid9560453.denovo.171, whole genome shotgun sequence.
ACCESSION   DACGLE010000159 DACGLE010000000
VERSION     DACGLE010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304449
            Sequence Read Archive: ERR985064
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1605)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1605)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702225.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702225.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 20:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,045
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 27 of 67
            Pseudo Genes (incomplete)         :: 25 of 67
            Pseudo Genes (internal stop)      :: 19 of 67
            Pseudo Genes (multiple problems)  :: 4 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1605
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304449-rid9560453.denovo.171"
                     /strain="VREN0534"
                     /db_xref="taxon:1352"
     gene            complement(1..81)
                     /locus_tag="IXT20_002828"
                     /pseudo
     CDS             complement(1..81)
                     /locus_tag="IXT20_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290351.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module antitoxin"
     gene            430..1515
                     /gene="xerS"
                     /locus_tag="IXT20_002829"
     CDS             430..1515
                     /gene="xerS"
                     /locus_tag="IXT20_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerS"
                     /protein_id="HAQ4861205.1"
                     /translation="MKNTEIYQKIDTIILHMPDYIKDFVQDREDKDQSPRTTLEYLKN
                     YKLFYEWLLSESIVPDTSITSIRQLTAYDLNLYKSHLKRRTKENVKKETTKTKLKDNN
                     NLGLSTSTINRNITALKVLFKYLSKSSNNPLGKPYLENNPMDQVATITDKTTLAARAN
                     AIEKKLFLDEDTQNYLDYIANEYKKTLSKRALIYYQRDVERDLAINALILGSGLRLSE
                     VVNINLEDLSLEKNNVVVTRKGNKRDAVNIAAFAMEYLANYLAIRKERYKVTENEKAL
                     FLAIYQGEAKRISGIAIERMVAKYSKGFRVQVSPHKLRHTLATRLYQQTNSLVLTAQQ
                     LGHTSTNTTTLYTHIDNAATIDALNAL"
BASE COUNT          611 a          275 c          219 g          500 t
ORIGIN      
        1 taaaaaaaat gtatctccag gatgcaaccc attttcttta agaaattcct tttaaattgt
       61 taatactact gagtgcccca cttctctgaa ttttctttaa cttctccctc ccctttttta
      121 tttgtatata cattcatata cgcttgttaa tatcaaatca accgtagtct ctcttatgtc
      181 taattatata gaaagattgt tctttctata taatttagac aatttaaaaa attaagattg
      241 cctccttctg tttcctttcc tattcatgta taccatttat tttaaattat taaatctata
      301 cctatttatc tgcttttttg aaataaatta ataaatttca aagtatacca attgacaaaa
      361 cactctataa ttggtatagt ccaattgtag agatactcct ctcccctact tatcgaaagg
      421 atgaataaca tgaaaaatac agaaatatat caaaaaatag atacaatcat acttcatatg
      481 cccgattata taaaagattt tgttcaagat cgtgaggata aagatcaatc acctcgaact
      541 actttagaat atctaaaaaa ttataagctc ttttatgaat ggcttctgag tgagtctatc
      601 gttcccgata cttcaatcac ctcgataaga cagttaactg cctatgatct aaacttgtac
      661 aaatctcatt tgaaacgtcg cacaaaggaa aatgtaaaaa aagagacaac aaaaactaag
      721 ctcaaagata ataataactt aggtctatct acttctacta tcaatagaaa tatcacagca
      781 ttaaaggttc tatttaaata tctttcgaaa agttccaata atcctttagg aaaaccatac
      841 ttagaaaata atcctatgga tcaagtagca actatcacag ataaaactac gttagctgcc
      901 cgtgcaaacg caatcgagaa aaaattattt ctagacgaag atacacaaaa ttatctagat
      961 tatattgcca atgaatataa aaaaactttg agcaaacgag ctttgatcta ttatcaacga
     1021 gatgtagaaa gagacctggc gatcaatgca ttgattttag gatctggatt aagactctcc
     1081 gaggttgtaa atattaattt agaggaccta tctttagaaa agaataatgt ggtagtaact
     1141 agaaaaggaa ataagagaga cgcagtaaat atagcagcat ttgcaatgga atatttagct
     1201 aattatctag ctattcggaa agaacgatac aaagtaacag aaaacgaaaa agctttattt
     1261 ctagcaattt atcaaggaga agccaagcgt atatccggta ttgcaatcga acggatggta
     1321 gcaaaatatt caaaaggatt cagagtccaa gtcagtccac ataaattacg tcatactttg
     1381 gctactagac tctatcaaca gacaaattca ttggtattaa ctgcccaaca attgggacat
     1441 acaagtacaa atacaacaac tttatatact catatagaca atgcagctac aattgatgct
     1501 ttgaacgctt tgtaaatgta gtaaaaaata gtataataat gaagcctgtc tctaaaatta
     1561 gataaaagcc caactttaaa ggcaagcttc attatcattt ttata
//
LOCUS       DACGLE010000160         1584 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0534
            SAMEA3304449-rid9560453.denovo.172, whole genome shotgun sequence.
ACCESSION   DACGLE010000160 DACGLE010000000
VERSION     DACGLE010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304449
            Sequence Read Archive: ERR985064
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1584)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1584)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702225.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702225.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 20:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,045
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 27 of 67
            Pseudo Genes (incomplete)         :: 25 of 67
            Pseudo Genes (internal stop)      :: 19 of 67
            Pseudo Genes (multiple problems)  :: 4 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1584
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304449-rid9560453.denovo.172"
                     /strain="VREN0534"
                     /db_xref="taxon:1352"
     gene            292..1479
                     /locus_tag="IXT20_002830"
     CDS             292..1479
                     /locus_tag="IXT20_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HAQ4861206.1"
                     /translation="MYFLGIDIGKRTHVASIMNEEGKVLLKGFSFPNTTEGAESLIER
                     MVDYSGAPSDFVIGMEATGHYWLSIFSYLHESDYLIHVVNPLQTDGWRKGTEIRKRKN
                     DIIDSVLIADLMRYGSFVETVLSDENVFSLKQLSRYRTYLVGTASDFKRKIIAVLDQV
                     FPEYATIFTKQGVFGKASKELLLAFSAPIDLENISSETLAQYLAELSRNRIKAEKADQ
                     LKQAASNSFGVKFAQEAFTFQLRSMIEQLKFIESQIKETEREIKHIMDTLNSVILTIP
                     GIGPINGATILGEIGDIQKFSNPKKLVAYAGIDASVTQSGQYEATHNVMSKRGSPYLR
                     KALFSAALVASQCDPVFSAFYQKKVSEGKHHLTALGAVSRKLCYVIHAILTKNEPYII
                     VSD"
BASE COUNT          467 a          295 c          316 g          506 t
ORIGIN      
        1 ttttaacctt ccggttttga ttggataacc ctatgaacca tcgttatttt aatttggtat
       61 taactcattt cttttgagat aatatattta ttggattgag aggctaagcc gttcccccct
      121 tgggtcattg aacccgcact cgagagactg gctcctctct ctgataagtg gattgcgtat
      181 gagttagatg ttgaaagtcc ttactttact ccgtatctcg tacaaggtag tgacgctttt
      241 cagttgagtg gatttcaatc caaaattctt tttttgaaag gtcagatata catgtacttt
      301 ttaggcattg atatcggtaa acgcactcac gttgcttcta tcatgaacga ggaaggaaag
      361 gtattactaa aaggtttttc atttcctaac accactgaag gagcagagtc attaattgag
      421 cgaatggtcg actattccgg agcacccagt gactttgtta tagggatgga agccacgggg
      481 cactattggc tgtccatctt ttcatacctt cacgaatcag attatttgat tcacgttgtg
      541 aatccccttc aaacagacgg atggcgtaaa ggtacagaaa tacggaaacg aaaaaacgat
      601 attattgatt ctgttttgat tgctgactta atgcgctatg gctcatttgt cgaaacggtc
      661 ttatccgatg aaaatgtttt ttctttgaaa caactttctc gttaccgaac ttatttagta
      721 ggtacagcga gcgacttcaa aagaaaaatt atagccgtat tggatcaagt ctttccagaa
      781 tacgcgacga tttttaccaa gcagggcgtt ttcggaaaag cttctaaaga gttattgctg
      841 gcgtttagtg ccccgatcga cttagaaaac atttcctcag aaactttggc ccaatactta
      901 gctgagttaa gcaggaaccg aatcaaggca gaaaaagcgg accaattgaa gcaagcagct
      961 tctaactcct ttggagtaaa atttgctcaa gaagcgttca ccttccaact acgttcaatg
     1021 attgaacaat taaaattcat tgaaagccaa ataaaggaaa cggaaagaga aatcaagcac
     1081 ataatggaca ctttaaattc tgttattctc acaattcctg gaattggtcc tataaacggt
     1141 gcgactattc ttggtgagat cggagatatt caaaaatttt ctaacccgaa aaaactcgtt
     1201 gcttacgctg ggatagacgc ttccgtgaca caatcagggc aatatgaggc aacgcataat
     1261 gttatgagta aacgagggtc tccatatttg cgaaaagcct tgttttcggc ggctttagta
     1321 gcttctcaat gtgatcctgt ttttagtgcg ttttaccaga aaaaagtttc cgaaggaaaa
     1381 catcatctaa ctgccttagg tgcggtttct cgtaagcttt gttacgtgat tcatgctatt
     1441 ttgactaaaa acgagcctta tataattgtt tctgattagc tattgaacat tcaaaaaaac
     1501 taatggtaag aagcttattt gttgtaccct tttttcggaa ttatctgtta taaaatattt
     1561 tttgatttgg ctaattttta cttg
//
LOCUS       DACGLE010000161         1570 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0534
            SAMEA3304449-rid9560453.denovo.173, whole genome shotgun sequence.
ACCESSION   DACGLE010000161 DACGLE010000000
VERSION     DACGLE010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304449
            Sequence Read Archive: ERR985064
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1570)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1570)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702225.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702225.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 20:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,045
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 27 of 67
            Pseudo Genes (incomplete)         :: 25 of 67
            Pseudo Genes (internal stop)      :: 19 of 67
            Pseudo Genes (multiple problems)  :: 4 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1570
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304449-rid9560453.denovo.173"
                     /strain="VREN0534"
                     /db_xref="taxon:1352"
     gene            43..102
                     /gene="ermCL"
                     /locus_tag="IXT20_002831"
     CDS             43..102
                     /gene="ermCL"
                     /locus_tag="IXT20_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002497446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methylase leader peptide ErmCL"
                     /protein_id="HAQ4861207.1"
                     /translation="MGIFSIFVINTVHYQPNKK"
     gene            162..896
                     /locus_tag="IXT20_002832"
     CDS             162..896
                     /locus_tag="IXT20_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011282421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(T)"
                     /protein_id="HAQ4861208.1"
                     /translation="MNKKNIKDSQNFITSKHHINEILRNVHLNTNDNIIEIGSGKGHF
                     TFELAKRCNYVTAIEIDPKLCRITKNKLIEYENFQVINKDILQFKFPKNKSYKIYGNI
                     PYNISTDIIRKIVFESTATESYLIVEYGFAKRLLNTNRSLALFLMTEVDISILSKIPR
                     EYFHPKPRVNSSLIVLKRHPSKISLKDRKQYENFVMKWVNKEYIKLFSKNQFYQALKY
                     ARIDDLNNISFEQFLSLFNSYKLFNR"
BASE COUNT          588 a          248 c          211 g          523 t
ORIGIN      
        1 gtgctataat tattctaatt tcataaaaag gagaattaat atatgggcat ttttagtatt
       61 tttgtaatca acacagttca ttatcaacca aacaaaaaat aagtggttat aatggattgt
      121 taatataaac attcattata acctcatagg agtgggttat aatgaacaaa aaaaatataa
      181 aagatagtca aaactttatt acttcaaagc atcatataaa tgaaattttg agaaatgtac
      241 atttaaatac aaatgataat attattgaga ttggttcagg gaaaggtcat ttcacgtttg
      301 aattagctaa aaggtgtaat tatgtaaccg ccattgaaat agatcctaaa ttatgtagga
      361 taactaaaaa caaacttatt gaatatgaga acttccaggt tatcaataaa gatattttac
      421 aatttaagtt tcctaaaaat aagtcatata agatatatgg aaatataccc tacaatataa
      481 gtacagatat aattcgaaaa attgtttttg aaagcacagc tacagaaagt tatttaatag
      541 tggaatatgg atttgctaaa aggttgctaa atacaaatcg ttcactagca ctatttttaa
      601 tgacagaagt tgatatatcc atattaagta aaatccctag agaatacttt catccaaaac
      661 ctagagttaa tagctcgtta attgtattaa aaagacaccc ttcaaaaata tcactcaaag
      721 atagaaaaca atatgaaaat tttgttatga aatgggttaa caaagaatac ataaaactat
      781 tttccaaaaa ccaattttat caagccttaa aatatgcaag aattgacgat ttaaacaata
      841 ttagctttga acaattcttg tctcttttca atagctataa attatttaat agataagtta
      901 aggggtgcat aaactgcatc ccttaactta tcttattttg cttaaacaat cttggcccgt
      961 aaactgcata actcatatgt aaatcgctcc tttttaggtg gcacaaatgt gaggcatttt
     1021 cactatttcc ggcaattact tccatgtaaa gtataaccca ctatactttg caagcaaagt
     1081 gtgggctcgc cgaggggtct tgcagacggc tctttcggct cttttgagcc gagtatcagc
     1141 gcacccttat ggtgcaatta gctgatacct gtttgacgat ttgctgccct accttttcgg
     1201 taggtatgca aacctcaaac caaaataaaa acccccttcg acttcgtcag ggggttttta
     1261 tttatataaa tctccgtaaa taatcgcgat ttaataacat cttcaatttt atttttttgc
     1321 gtattttaga aatgttttca ctaaaaaata tattatacaa aaaatacagt aatcgattat
     1381 aattctttgc cagttaaata tcatatcgtt attaaaatat cggaaaaaaa tatcaacaac
     1441 aataaatgca aaagaagacc ataaaaattg tttattactc atacatactc tccatttcta
     1501 tcatttttaa atttaccagt catctctcaa aaatctccta gctatttttc ttttttaacc
     1561 acataccgtc
//
LOCUS       DACGLE010000162         1478 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0534
            SAMEA3304449-rid9560453.denovo.174, whole genome shotgun sequence.
ACCESSION   DACGLE010000162 DACGLE010000000
VERSION     DACGLE010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304449
            Sequence Read Archive: ERR985064
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1478)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1478)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702225.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702225.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 20:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,045
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 27 of 67
            Pseudo Genes (incomplete)         :: 25 of 67
            Pseudo Genes (internal stop)      :: 19 of 67
            Pseudo Genes (multiple problems)  :: 4 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1478
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304449-rid9560453.denovo.174"
                     /strain="VREN0534"
                     /db_xref="taxon:1352"
     gene            complement(<1..156)
                     /locus_tag="IXT20_002833"
     CDS             complement(<1..156)
                     /locus_tag="IXT20_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAQ4861209.1"
                     /translation="MSRRQRRTYSKEFKQQIVDLYLAGKPRAEIIREYELTPSSFDKW
                     MKQAQSTG"
     gene            431..973
                     /locus_tag="IXT20_002834"
     CDS             431..973
                     /locus_tag="IXT20_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4861210.1"
                     /translation="MLLKVAIKCYCVKNKGGIIYEKIVPRGACLLLALCLSLSAVGCQ
                     KSNENNKIKEGQSISSEEEGMSINANGKNETFKPSDYTLEAKKEYVYEYLGLKFKLSD
                     KFRNYIADKKIAMLDDQSPIDKELKYAILTFEKMTEEQKNAVIEKMGDGYKNWQNELE
                     RIGTIGIFEKNTSEEKNLKL"
     gene            1063..>1478
                     /locus_tag="IXT20_002835"
     CDS             1063..>1478
                     /locus_tag="IXT20_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
                     /protein_id="HAQ4861211.1"
                     /translation="MDELKRTEIQIIDKKERPKNGFVLSEKTDLENTEAFNKESVKDL
                     RKLPTKDINGKDFTSKDFEKYDLTMVNVFATWCTACVKEIPDLVEVQNEMKSKGVNIV
                     GVVTDPVDDNGENKEAIEKSKLIHEKTKASYPFLMP"
BASE COUNT          570 a          186 c          284 g          438 t
ORIGIN      
        1 gcccgttgat tgtgcttgct tcatccattt atcgaaagaa gaaggcgtaa gctcatactc
       61 tcgaataatt tctgcgcgag gcttaccagc gagatagaga tcgacgattt gttgtttgaa
      121 ttcttttgaa taggttcttc gttgacgtct tgacatgaaa aatcctccag tgtgtttttt
      181 attattctac acaccttatt ttttctgtct agtctagtgt agccgattca aattgatgaa
      241 gcagatccca aagggctgct tcatcaattt tttcgtaagt gtccgtacac ttactgtgct
      301 tgctcatata ttaatcgatc atatactaat cgtgtaagaa caactttaag cataatactt
      361 atattgttat accatagttt ggaaaaataa aaaattatat ttcatcaacg tttcataaaa
      421 ggtgtgtaga atgttattga aagtagctat aaaatgctat tgtgtaaaaa ataaaggagg
      481 aataatttat gaaaaaatag taccaagagg agcatgccta ttacttgcat tatgtttgag
      541 cttaagcgca gttgggtgtc aaaaaagtaa tgaaaacaat aaaataaaag aaggacaatc
      601 cataagttct gaagaggaag gcatgtcaat taatgcaaat ggcaaaaatg aaacttttaa
      661 gccatcagat tatacattgg aggcaaagaa agaatatgtt tatgaatatt tagggcttaa
      721 atttaaactc tcagacaaat ttagaaatta tatagctgat aaaaagatag cgatgttaga
      781 tgatcaaagt cctatagata aagaactaaa gtatgctatt ttgacatttg agaaaatgac
      841 agaagaacaa aagaatgcag ttatagaaaa gatgggcgat ggatataaaa attggcaaaa
      901 tgagcttgaa agaattggga ctattggtat atttgaaaaa aatacatcag aagaaaaaaa
      961 tctaaaatta taaaatgtga tactcatact aaaataggag tttcaagtga tggaaaatac
     1021 gattgctatt taagcacaaa tagcggagct gaaagtaatc ttctggatga attaaaaaga
     1081 actgagattc aaattataga taaaaaagaa cgtcctaaaa atggttttgt gctatcagaa
     1141 aaaactgatt tagaaaatac agaagcattt aacaaagaat cggttaaaga tttacgtaaa
     1201 ctaccaacaa aggacataaa tggcaaggat tttacaagta aagattttga gaagtatgat
     1261 cttactatgg taaatgtatt tgcaacttgg tgtacagctt gtgtaaagga aattcctgat
     1321 ttggttgagg ttcagaatga aatgaaaagc aagggtgtaa atatcgttgg agttgttaca
     1381 gatcctgttg atgataacgg tgaaaacaag gaagcaattg aaaagtcaaa gttaatacat
     1441 gaaaagacaa aggcttcata tcctttctta atgccaga
//
LOCUS       DACGLE010000163         1456 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0534
            SAMEA3304449-rid9560453.denovo.175, whole genome shotgun sequence.
ACCESSION   DACGLE010000163 DACGLE010000000
VERSION     DACGLE010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304449
            Sequence Read Archive: ERR985064
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1456)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1456)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702225.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702225.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 20:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,045
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 27 of 67
            Pseudo Genes (incomplete)         :: 25 of 67
            Pseudo Genes (internal stop)      :: 19 of 67
            Pseudo Genes (multiple problems)  :: 4 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1456
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304449-rid9560453.denovo.175"
                     /strain="VREN0534"
                     /db_xref="taxon:1352"
     gene            108..1040
                     /locus_tag="IXT20_002836"
     CDS             108..1040
                     /locus_tag="IXT20_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4041 domain-containing protein"
                     /protein_id="HAQ4861212.1"
                     /translation="MLKLKEKEYIIDDAVSVPLILNKREKNNLKKKMLIPFDSEVKGL
                     LNKLTISNIDSTREKIIKIFDKINNLFKNDNAELRKGLLEFKLQELELHYSYLVKITE
                     EKEQQKAIKEQMIEEEKVRREIDREKKRIDKEQRQFNSEISKLIAYMQKANADVEKEL
                     YANKIQELEEKLKELEVIRENVLQRELNTRAGYVYVISNIGSFGEDIYKIGMTRRLEP
                     MDRIKELSSASVPFEFDVHAMIFSEDAPSLETKLHNHFRKQEVNKINQRKEFFKVSLD
                     EIEKVVLENYNGTVTFTKLAKAEQYRRSLELSKD"
     gene            1112..>1456
                     /locus_tag="IXT20_002837"
     CDS             1112..>1456
                     /locus_tag="IXT20_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4861213.1"
                     /translation="MWLEKINDKKFKYTERYTDPLTEKKRKVSVTLSSNSRQAWNQAN
                     LLLNEKIAEKIKRNEELPLTFGELKTKWDEKYKPTVKESSYRTTQVYLSLISKYIKDD
                     VLVKNVNSNLIQD"
BASE COUNT          640 a          169 c          227 g          420 t
ORIGIN      
        1 atgaaataaa tcaaaagtat gatgaaacat taaaaaatgt ctattcttca cttatcgttt
       61 caaatataga agaaacttca tcttctgaaa taaaaaataa gctttctttg ttaaaattaa
      121 aagaaaagga atatattatt gacgatgctg tttctgtccc tcttatttta aataaacgag
      181 agaaaaataa tttaaagaaa aaaatgctca ttccatttga ttcagaagtg aaaggattac
      241 tcaataaact aactatttct aatatagact cgactaggga aaaaatcata aaaatatttg
      301 ataaaattaa taatttattc aagaatgaca atgctgaatt aaggaagggt cttttagagt
      361 ttaaacttca agaactagaa cttcattact cttatttagt gaaaattacc gaagaaaaag
      421 aacaacaaaa agcaataaaa gaacagatga tagaagagga aaaagttcgt agagaaattg
      481 atcgtgaaaa gaaacgtatt gataaagaac aacgtcaatt caattctgaa atttccaaat
      541 taatcgcata tatgcaaaaa gctaatgcag atgtggaaaa agaattatat gcaaataaaa
      601 ttcaagaact ggaagaaaaa ttaaaagaat tagaagttat acgtgaaaat gtattacaaa
      661 gagaattaaa tactagagcc ggttatgtgt atgtaattag taatattggg tcttttggtg
      721 aagacatata taaaattggt atgactagaa gattagaacc tatggataga ataaaagaat
      781 tgagtagtgc atcggttcca tttgaatttg acgttcatgc gatgattttt tcagaggacg
      841 ctccgtcttt ggaaactaaa ttgcataatc attttagaaa acaagaagtt aataaaatta
      901 atcaaagaaa agaatttttc aaagtttcac tagacgaaat agagaaagtt gttttagaaa
      961 attataatgg aacagtaaca tttacaaaat tagctaaagc tgaacaatat agacgatctt
     1021 tagaattgtc aaaagattaa atatataaaa agccttcggg ctttttcttt taacaattaa
     1081 taggaacgta tattcgaaag gaatagaaat aatgtggtta gaaaaaataa atgacaaaaa
     1141 atttaagtac accgaacgtt atacggatcc cttaactgaa aaaaagagaa aagtgtccgt
     1201 tactttatct agcaattcac gccaagcttg gaatcaagct aacttacttt tgaatgaaaa
     1261 aatcgcagaa aaaataaaac gaaatgaaga attaccacta acttttggag aattaaaaac
     1321 taaatgggat gaaaaataca agccaactgt aaaagaaagt tcctatcgaa caactcaggt
     1381 ttatctatca ttaatttcaa aatatataaa agatgatgta cttgtaaaaa acgttaatag
     1441 taatcttata caagat
//
LOCUS       DACGLE010000164         1445 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0534
            SAMEA3304449-rid9560453.denovo.176, whole genome shotgun sequence.
ACCESSION   DACGLE010000164 DACGLE010000000
VERSION     DACGLE010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304449
            Sequence Read Archive: ERR985064
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1445)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1445)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702225.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702225.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 20:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,045
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 27 of 67
            Pseudo Genes (incomplete)         :: 25 of 67
            Pseudo Genes (internal stop)      :: 19 of 67
            Pseudo Genes (multiple problems)  :: 4 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1445
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304449-rid9560453.denovo.176"
                     /strain="VREN0534"
                     /db_xref="taxon:1352"
     gene            <1..226
                     /locus_tag="IXT20_002838"
     CDS             <1..226
                     /locus_tag="IXT20_002838"
                     /inference="COORDINATES: protein motif:HMM:NF018513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HAQ4861214.1"
                     /translation="LWALGIMLVAFIVAKKNKYPVVEKGQMAHAGKIIKEAIPSLLLI
                     VIIIGGILTGIFTAVEASAIAVIYKWVIRF"
     gene            208..513
                     /gene="kduI"
                     /locus_tag="IXT20_002839"
                     /pseudo
     CDS             208..513
                     /gene="kduI"
                     /locus_tag="IXT20_002839"
                     /inference="COORDINATES: protein motif:HMM:NF002091.0"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
     gene            533..1336
                     /locus_tag="IXT20_002840"
     CDS             533..1336
                     /locus_tag="IXT20_002840"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HAQ4861215.1"
                     /translation="MEFSMDMFRLDGKVALVTGAVYGIGFEIARSLATAGAKIVFNNL
                     NQESVDEGIAHYKEAGIDAKGYVCDVTDEEAVQAMVQQIKEDVGSVDILVNNAGIIKR
                     TPMIEMDAADFRQVIDVDLNAPFIVSKAVIRDMIEKGGGKIINICSMMSELGRETVSA
                     YAAAKGGLKMLTKNIASEYGQYNIQCNGIGPGYIATPQTAPLRETQENGERHPFDQFI
                     VGRTPAARWGEPIDLAGPSVFLASSASDFVNGHVLYVDGGILAYIGKQP"
BASE COUNT          462 a          259 c          344 g          380 t
ORIGIN      
        1 tttgtgggcg ctaggaatca tgctagtagc attcatcgtt gctaaaaaga ataaataccc
       61 tgttgtcgaa aaaggacaga tggctcatgc tggaaaaata ataaaagagg cgattcctag
      121 ccttctattg attgtcatca ttatcggtgg tatcttgacg gggatattta cagcagtaga
      181 agcttcagcg atagctgtca tttataaatg ggttatacga ttttagaacc aggaagtgct
      241 tggaatacga tgccttgtca tacacatgaa cgtcgtatgg aagcttatgt ctattttgat
      301 tatgccaatg aagatacacg tgtgttccat atgatgggca aaccagatga aacaaaacat
      361 ttagtcgtag acaatgaaca agccattatt tcgccaagct ggtcgatcca ttcaggtgta
      421 ggtacaagca attactcatt catctgggca atgtgcggag aaaatatcac ctatacagac
      481 atggatatgg tgcaaatgga tcaattaaaa taagcataag ggagagaaaa tgatggaatt
      541 tagtatggat atgtttcgtt tagatggaaa agtagcatta gtcaccggtg ctgtctatgg
      601 tattggattt gagattgcac gctctttagc tacagcagga gcaaaaatcg tatttaataa
      661 cttgaatcaa gaatcagttg atgaaggtat agcgcattat aaagaagcag gcatcgatgc
      721 aaaaggttat gtctgcgatg tgaccgatga agaagcggtt caagcgatgg ttcaacagat
      781 caaagaagat gtaggttcag ttgatattct agtcaacaat gcagggatca tcaaacggac
      841 accgatgatc gaaatggatg cggcagactt ccgccaagta atcgacgtgg atttaaatgc
      901 cccattcatc gtttcaaaag cagttattcg ggatatgatc gaaaaaggcg gcggtaaaat
      961 catcaatatc tgctccatga tgagtgaatt aggacgcgaa acggtcagtg cttatgctgc
     1021 ggcaaaaggc ggattaaaaa tgctgacgaa aaacatcgct tctgaatacg gtcaatacaa
     1081 tatccaatgt aatggaattg gaccaggtta tattgctaca ccgcaaactg ctccattacg
     1141 cgaaacacaa gaaaacggtg aacgtcatcc attcgatcaa tttatcgttg gacgtacgcc
     1201 agcagcacgc tggggcgagc caatcgattt ggctggacca tctgtcttct tggcttctag
     1261 cgcatctgat ttcgttaacg gccatgtctt gtatgtggac ggaggcatac ttgcctatat
     1321 cggaaaacaa ccttaaaaga gctttagtac accgtttagt ggagaggttg tgaaatcagc
     1381 ggcttgaacg aataccgaag gacagctgat taaacatcat ttattcgaaa aaagaggctg
     1441 ggaca
//
LOCUS       DACGLE010000165         1424 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0534
            SAMEA3304449-rid9560453.denovo.177, whole genome shotgun sequence.
ACCESSION   DACGLE010000165 DACGLE010000000
VERSION     DACGLE010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304449
            Sequence Read Archive: ERR985064
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1424)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1424)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702225.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702225.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 20:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,045
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 27 of 67
            Pseudo Genes (incomplete)         :: 25 of 67
            Pseudo Genes (internal stop)      :: 19 of 67
            Pseudo Genes (multiple problems)  :: 4 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1424
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304449-rid9560453.denovo.177"
                     /strain="VREN0534"
                     /db_xref="taxon:1352"
     gene            complement(150..1337)
                     /locus_tag="IXT20_002841"
     CDS             complement(150..1337)
                     /locus_tag="IXT20_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS16 family transposase"
                     /protein_id="HAQ4861216.1"
                     /translation="MTQVHFTFESEEIQAIINESGANDTAKTLMTIMFNQLMEEQRNQ
                     YIQANAYERSEERQSQRNGYYDRSFTTRIGTLELHVPRTRDGKFSPTIFERYQRSEKA
                     LIAAMIEMVISGVSTRKVTKTVELLTDGATVSKSFVSNLMKQLDPFVFEWRNRSLEGS
                     EYPFFMCDALYMKVRENHRIVSKGVYIGIGIDSDGRRTILGFDVQDGESEDNWDTVFQ
                     SFVQRGLFGVKLVISDAHKGLVKAVRKNFLGASWQRCQAHFLRNIFDKLPKKVSSDVK
                     DELKSIFKASELELTRERKEHFLEKYGCDSKLSAACDILENGFEDAIQILSFPENIRR
                     RIRTTNVLERLNEEIRRRERVIRIFPNINSITRIIGTLLMEKDTEWLASPRKYLEFNS
                     NNI"
BASE COUNT          414 a          309 c          249 g          452 t
ORIGIN      
        1 taaaagttca acctcatcaa aagcacaatg ttttgcgttg tgaacctaac tgccatgcct
       61 ttctttctga ggagagaggt caggagcagt agcgcagcga ctcttgatgt ctctcctcag
      121 aaagaagaga ctcgcacagg ttcatggtgc taaatgttat tcgaattaaa ttctaaatat
      181 tttcgtggag aagccagcca ctcagtgtct ttttccatca aaagtgttcc aattatccga
      241 gtgatggaat tgatatttgg aaaaatccga atcactcgtt ctctccggcg aatctcttcg
      301 ttcaaccgtt ccaacacatt ggttgtacga atccttcggc ggatattttc aggaaaagac
      361 aaaatttgaa tagcatcttc aaagccgttt tctaaaatgt cgcaagcagc acttaatttt
      421 gaatcacaac catatttttc caagaagtgc tcttttcttt cacgtgtcaa ttcaagttca
      481 gaggctttaa agatactctt caactcgtct tttacatcag aagaaacttt ctttggcagc
      541 ttatcaaaaa tatttctcaa aaagtgggct tggcatcttt gccagctcgc acctaaaaag
      601 ttcttgcgaa cagccttaac taaccctttg tgtgcatcag aaatgaccag ttttacaccg
      661 aacaaacctc gttgaacgaa cgattgaaaa acggtatccc aattatcttc cgattcgccg
      721 tcttgaacat caaaaccaag aatcgtacgt cgtccatcag aatcaatgcc gataccaata
      781 tatacacctt ttgagacaat acgatgattt tcccttactt tcatatacag ggcatcacac
      841 atgaaaaatg gatattccga gccttctagg ctccggtttc tccactcaaa aacaaatgga
      901 tccaactgtt tcatcagatt tgaaacgaat gatttagaaa cagtcgcacc atccgttagc
      961 agttcaactg ttttagttac tttgcgagtg gagacaccag aaatgaccat ttcaatcatg
     1021 gcagcgatca aagctttttc gcttcgctga tatctttcaa agatagtagg actaaactta
     1081 ccgtctctgg ttcgtggaac atgaagttct aacgtaccaa ttcgagttgt aaacgaacga
     1141 tcgtagtaac catttctttg tgattgccgt tcttctgaac gttcataggc atttgcctga
     1201 atatattgat ttcgttgttc ttccatcagc tgattaaaca tgatagtcat aagtgttttt
     1261 gcggtatcat tcgcaccgct ttcgtttata attgcttgaa tttcttcgct ttcaaaagta
     1321 aaatgtactt gagtcattga tatcagtcct attcgagtat gttttctgca aaaaacattg
     1381 tactcctaac tgagatcaat ggcttttttg atgcttcact ttta
//
LOCUS       DACGLE010000166         1406 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0534
            SAMEA3304449-rid9560453.denovo.178, whole genome shotgun sequence.
ACCESSION   DACGLE010000166 DACGLE010000000
VERSION     DACGLE010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304449
            Sequence Read Archive: ERR985064
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1406)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1406)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702225.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702225.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 20:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,045
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 27 of 67
            Pseudo Genes (incomplete)         :: 25 of 67
            Pseudo Genes (internal stop)      :: 19 of 67
            Pseudo Genes (multiple problems)  :: 4 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1406
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304449-rid9560453.denovo.178"
                     /strain="VREN0534"
                     /db_xref="taxon:1352"
     gene            97..1398
                     /locus_tag="IXT20_002842"
     CDS             97..1398
                     /locus_tag="IXT20_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HAQ4861217.1"
                     /translation="MPDLHHIKLLLGIKDKNIFITNVESKSIKNIKSLVVSATLSKEI
                     RRCPLCKQMNHEGMIVKNGKKKSLIQLNKCANQLTYLALAKQRYHCRGCHTYFTANTY
                     IVDRNCFIAKQVRYKILEELTEKQAMTTIAKHCGVSWSTVSRTLASLIPMTKVKRNWL
                     PRCLLMDEFRSLKNQVGPYSFSCMDGDTGKLLDILPSRKKKALVSYFMQFERRARLKV
                     KVIVTDMNVSYASLIKECFPKAKLVVDRFHIVKHLIRKFEDIRVRIMKSFDRNDPTQA
                     KYYRQLKALSRLLIKRQDKLVYDKWTKWRNFGWAYLTESEVVDRLLSISDELRIAYFY
                     YQEILQAFHDKEADTFFKLVRTMPNSVPKELHSIKKAFINYESGIRLALELPYSNAKI
                     ENLHTHIKALKRVAYGFRSFRKMKTRIFLLNNLITYESKNI"
BASE COUNT          462 a          284 c          258 g          402 t
ORIGIN      
        1 atcattctat cggtcccttt tgagcatgaa aaaacaaaac atacagaagc tccgttagaa
       61 ttgaatcacc acaacacaat caaaggagcg ttctgtatgc ctgatcttca tcatatcaaa
      121 ctactacttg gaatcaaaga taaaaacatt ttcatcacaa atgtggaatc gaagagcatt
      181 aagaatatca agagtcttgt tgtttctgcg acgttaagta aagaaattcg tcgctgtcct
      241 ttgtgtaaac agatgaatca tgaaggcatg atcgttaaga acggaaagaa aaaatcgctc
      301 attcaactta ataaatgtgc gaaccaactg acttatcttg ctttagcgaa acaacgctac
      361 cactgtcgtg gctgccacac ctattttacg gcgaatacct acattgtcga tagaaactgc
      421 tttatcgcta aacaagtgcg ctataaaatt ttagaagagc taactgaaaa acaagcaatg
      481 acgacaattg ctaagcattg cggcgtttct tggtcgaccg tttctcggac actcgcttct
      541 ctgatcccca tgacaaaagt aaaaagaaat tggttaccgc gttgtttatt aatggatgaa
      601 tttcgttcac tgaaaaatca agtcggtccc tactcctttt cttgtatgga tggcgatact
      661 ggaaaattac tggatattct tccttcacgg aagaaaaagg ctctcgtttc ttattttatg
      721 caatttgaac gacgcgcacg attgaaggta aaggtcatcg tgacggacat gaatgtttct
      781 tatgcctctc tgataaagga atgttttcca aaggcaaaac tcgttgtgga tcgttttcac
      841 attgtcaaac atctcattcg taaatttgag gatattcgcg tacgaatcat gaaaagcttt
      901 gatcgaaacg atccaacaca agccaaatat tatcgacaat taaaggcgtt gtctcggctt
      961 ctgatcaaac gacaggacaa gcttgtttat gacaaatgga ccaaatggcg caattttggt
     1021 tgggcgtatc tcaccgaaag tgaagtggtg gatcgtctgc tttcgatctc tgacgaatta
     1081 cgaatcgcct atttttacta tcaagaaatt cttcaagcgt ttcacgacaa agaggcggac
     1141 actttcttta aacttgtaag aacgatgccc aattccgttc caaaagaact gcattctata
     1201 aaaaaagctt ttataaacta tgaatccggc attcgtttag ccctcgaatt gccttattca
     1261 aatgccaaaa tagaaaatct gcatacccac atcaaagcat tgaaacgagt cgcttatggc
     1321 tttcgaagct ttcggaaaat gaagaccaga attttcttac taaataatct gattacctat
     1381 gaatcaaaaa acatctaaag tagaaa
//
LOCUS       DACGLE010000167         1354 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0534
            SAMEA3304449-rid9560453.denovo.179, whole genome shotgun sequence.
ACCESSION   DACGLE010000167 DACGLE010000000
VERSION     DACGLE010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304449
            Sequence Read Archive: ERR985064
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1354)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1354)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702225.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702225.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 20:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,045
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 27 of 67
            Pseudo Genes (incomplete)         :: 25 of 67
            Pseudo Genes (internal stop)      :: 19 of 67
            Pseudo Genes (multiple problems)  :: 4 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1354
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304449-rid9560453.denovo.179"
                     /strain="VREN0534"
                     /db_xref="taxon:1352"
     gene            82..>1354
                     /locus_tag="IXT20_002843"
     CDS             82..>1354
                     /locus_tag="IXT20_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element ISEfa11 family transposase"
                     /protein_id="HAQ4861218.1"
                     /translation="MNDSIKKMLRITEKDLMITEVSYETLQKKKTLVVDAVLSPTPRA
                     CRSCGSTVVDGNGKAIIVKNGKKETIVRFEQYNHMPLVMRLKKQRYTCKNCRTHWTAQ
                     SYFVQPRHSIANHVRYKIASLLTEKVSLSFIAKSCQVSLTTVIRTLKEFKSYLPKQSK
                     KILPRVLMVDEFRSHASIEDKMSFICADGETGKLIDVLPTRKLPRLTSYFLGCTNPEE
                     VEFLVTDMNAAYFQLTKRVLPNAKVVIDRFHIVKHMNQAFNELRIREMNELRKAGQKS
                     QAEKLKKNWRFLLKNRANINHYEYKTWKSFRAPKYPFLTEAMMIDRLLEFSPPLKEAY
                     PFFHELVEAFRDKDPDLFFSLLAELPETLDDSFREKLQNLLTYEEGITNAMIYPYSNG
                     KIEAKNTHIKTMKRVSYGFKSFENMRIRIFLI"
BASE COUNT          460 a          261 c          257 g          376 t
ORIGIN      
        1 attttaggta ttaaaaaaca tatcgttctc tagtatgatt aaatcaccac aaaataaaca
       61 actgagagga cgatacgcta tatgaatgat tctatcaaaa aaatgctgag aataacagag
      121 aaagatttga tgattacaga ggtctcttac gagacccttc agaagaaaaa gacgttggtc
      181 gtcgatgctg ttctctcgcc tactcctcgt gcttgtagaa gttgtggttc tactgtggta
      241 gatggaaacg ggaaagcaat tatagtgaaa aatggaaaaa aggaaacgat tgtccgtttt
      301 gaacaataca atcatatgcc tttggttatg cgcctaaaaa agcaacgcta tacctgtaaa
      361 aactgtcgca cccattggac ggctcaaagt tattttgtcc aacccagaca ttcaatcgca
      421 aatcatgtta gatataaaat tgcttcttta ctgactgaaa aagtatcttt atcttttatt
      481 gcgaaaagct gtcaggtatc tttgaccacc gttattcgta cattgaaaga gtttaaaagc
      541 tatttaccaa agcaatctaa gaagatcctc cccagagtat tgatggttga cgaatttcgt
      601 tcgcatgctt ccatagaaga taagatgagc tttatttgcg cggatggcga aacaggaaaa
      661 ttaatagatg ttttgcctac gcgtaaatta cctcgattaa caagctattt cttaggttgt
      721 accaatccag aagaagtaga attcttggtg acagacatga acgccgccta cttccagctc
      781 accaaacgtg ttctgccaaa tgcgaaagtc gtgattgatc ggtttcatat tgtcaaacac
      841 atgaatcaag cgttcaatga gttgcgtatc cgtgaaatga atgaacttcg taaagccgga
      901 cagaaaagcc aggcagaaaa actgaaaaag aactggcgct ttttgctaaa aaatcgtgca
      961 aacatcaacc attatgaata caaaacatgg aaaagtttcc gagcaccaaa atacccattt
     1021 cttactgaag caatgatgat tgatcgattg cttgagtttt ctccgcccct aaaggaggcg
     1081 tatccctttt ttcatgaatt agttgaagcc tttcgagaca aagaccctga cttatttttc
     1141 tccttattgg cagaacttcc cgaaacgtta gatgacagct ttcgggaaaa gcttcaaaac
     1201 cttctgacct atgaagaagg catcaccaac gcaatgatct atccttattc caatggaaaa
     1261 atagaagcga agaataccca cataaagaca atgaaacgag tatcctacgg atttaaatca
     1321 tttgagaaca tgagaattag aatctttttg atca
//
LOCUS       DACGLE010000168         1337 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0534
            SAMEA3304449-rid9560453.denovo.180, whole genome shotgun sequence.
ACCESSION   DACGLE010000168 DACGLE010000000
VERSION     DACGLE010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304449
            Sequence Read Archive: ERR985064
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1337)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1337)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702225.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702225.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 20:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,045
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 27 of 67
            Pseudo Genes (incomplete)         :: 25 of 67
            Pseudo Genes (internal stop)      :: 19 of 67
            Pseudo Genes (multiple problems)  :: 4 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1337
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304449-rid9560453.denovo.180"
                     /strain="VREN0534"
                     /db_xref="taxon:1352"
     gene            <1..>1337
                     /locus_tag="IXT20_002844"
     rRNA            <1..>1337
                     /locus_tag="IXT20_002844"
                     /product="23S ribosomal RNA"
BASE COUNT          358 a          293 c          406 g          280 t
ORIGIN      
        1 gaaataatag taccgaagtt cctgatgtca cactgccgag aaaagcttct agtgagaaaa
       61 cagcggcccg taccgcaaac cgacacaggt agtcgaggag agaatcctaa ggtgagcgag
      121 agaactctcg ttaaggaact cggcaaaatg accccgtaac ttcgggagaa ggggtgctga
      181 tcatacgatc agccgcagtg aataggccca agcgactgtt tatcaaaaac acaggtctct
      241 gcaaaatcgt aagatgaagt ataggggctg acgcctgccc ggtgctggaa ggttaagagg
      301 agtgcttagc gcaagcgaag gtacgaattg aagccccagt aaacggcggc cgtaactata
      361 acggtcctaa ggtagcgaaa ttccttgtcg ggtaagttcc gacccgcacg aaaggcgtaa
      421 cgatttgggc actgtctcaa cgagagactc ggtgaaattt tagtacctgt gaagatgcag
      481 gttacccgcg acaggacgga aagaccccat ggagctttac tgtagtttga tattgagtgt
      541 ctgtaccgca tgtacaggat aggtaggagc cgtagaaatc ggaacgctag tttcgatgga
      601 ggcgctggtg ggatactacc cctgcgttat ggccactcta acccgcacca ctaatcgtgg
      661 tgggagacag tgtcagatgg gcagtttgac tggggcggtc gcctcctaaa aggtaacgga
      721 ggcgcccaaa ggttccctca gaatggttgg aaatcattcg aagagtgtaa aggcagaagg
      781 gagcttgact gcgagaccaa caagtcgagc agggacgaaa gtcgggctta gtgatccggt
      841 ggttccgcat ggaagggcca tcgctcaacg gataaaagct accctgggga taacaggctt
      901 atctccccca agagtccaca tcgacgggga ggtttggcac ctcgatgtcg gctcgtcgca
      961 tcctggggct gtagtcggtc ccaagggttg ggctgttcgc ccattaaagc ggcacgcgag
     1021 ctgggttcag aacgtcgtga gacagttcgg tccctatccg tcgcgggcgt tggaaatttg
     1081 agaggagctg tccttagtac gagaggaccg ggatggactt accgctggtg taccagttgt
     1141 tctgccaagg gcattgctgg gtagctatgt agggaaggga taaacgctga aagcatctaa
     1201 gtgtgaagcc cacctcaaga tgagatttcc catttcttta agaaagtaag atccctgaga
     1261 gatgatcagg tagataggtc aggagtggaa gtacagtgat gtatggagcg gactgatact
     1321 aatcgatcga ggactta
//
LOCUS       DACGLE010000169         1328 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0534
            SAMEA3304449-rid9560453.denovo.181, whole genome shotgun sequence.
ACCESSION   DACGLE010000169 DACGLE010000000
VERSION     DACGLE010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304449
            Sequence Read Archive: ERR985064
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1328)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1328)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702225.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702225.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 20:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,045
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 27 of 67
            Pseudo Genes (incomplete)         :: 25 of 67
            Pseudo Genes (internal stop)      :: 19 of 67
            Pseudo Genes (multiple problems)  :: 4 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1328
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304449-rid9560453.denovo.181"
                     /strain="VREN0534"
                     /db_xref="taxon:1352"
     gene            <1..>1328
                     /locus_tag="IXT20_002845"
     CDS             <1..>1328
                     /locus_tag="IXT20_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HAQ4861219.1"
                     /translation="LFSDEEISVSVPEAAATIIPVKGHQRVVGTKADKIKHLPITEKE
                     HLLPLEEQFCEHCGSQMKDIGRTKVREEIRFHQAMLDCLTHYQHTYCCKSCEKEGLSS
                     FKKAIVPKPLISNSLGSNSLVAETIRMKFGQKVPAYRQENYWKQTHGLDISRDNITNW
                     HIKAVQNALDPLGERLRVYLNQEEILHGDETSYRVIESAKTDTYYWQFCTGKDSQHPI
                     VYYHHDESRAGDVPKTFLKEFTGYLHCDGYSGYNAVESVRLVYCFAHVRRKFFEAIPK
                     GKKNTDIPAAQAVKQLDKWFVLEKKWKDFSPEKRLSCRQQELRPLFIAFYEWMATIDP
                     VAKSKLDAAVQYACKLRSGFEPIFEDGRLELTNNRAERNIKELVIGRKNWLHSTSLEG
                     ARTSGIILSVYKTAELNGLNPVKYLEFLFDKIPNLPVLSAETLDQLLPWN"
BASE COUNT          441 a          238 c          286 g          363 t
ORIGIN      
        1 ctttttagtg atgaagaaat ttctgtaagt gttccagaag cagctgccac cattattcca
       61 gtaaaaggac atcaacgagt tgtaggaacg aaagcagaca aaatcaaaca cttacctata
      121 acagagaaag aacatctcct tcctttggaa gaacaatttt gtgaacattg cggttcacag
      181 atgaaagata tcggacggac aaaagtacga gaagaaattc gtttccatca ggctatgttg
      241 gattgtctca cccattatca acatacctat tgttgtaaaa gctgcgaaaa agaaggactt
      301 tcttctttta aaaaagcgat cgttcctaaa cctttgattt ctaatagctt aggttccaat
      361 agtttagtgg cggaaacgat ccggatgaaa tttggtcaga aagttcctgc ctatcggcaa
      421 gagaactatt ggaaacaaac acatgggttg gatatttcga gagataatat caccaattgg
      481 catattaaag cagtgcaaaa cgcgttagac cctttaggag aacgattaag agtctatctt
      541 aatcaagaag agattctaca cggggatgaa acaagttatc gtgtgattga aagtgccaaa
      601 acagatacct attactggca gttttgtaca ggaaaagaca gtcagcatcc catcgtttat
      661 tatcaccacg acgaaagtcg tgctggagat gttccgaaaa cttttctcaa ggagtttaca
      721 gggtatttac actgtgacgg ctatagcggg tataatgccg tggaaagtgt acgacttgtc
      781 tattgtttcg cgcatgttcg gcggaaattt ttcgaagcaa ttccaaaagg aaagaaaaac
      841 acggatattc ccgcagctca agcagtcaaa caattggata aatggtttgt cctagaaaag
      901 aaatggaaag acttttctcc tgagaagcga ttaagctgtc ggcaacaaga actgcgtcca
      961 ttatttattg ctttttatga gtggatggca acgatagatc ctgttgcaaa atccaagttg
     1021 gatgcagccg ttcaatatgc gtgtaaacta cgaagtggtt ttgaacctat ttttgaagat
     1081 ggtcgtttag aactcacaaa taatcgtgcg gagcgaaata tcaaagagct ggtgatagga
     1141 cggaaaaatt ggttgcattc cacaagtctt gaaggagcac gaacttcagg aatcattctg
     1201 agtgtatata aaacagcaga acttaacgga ttgaatcctg taaagtatct agagtttcta
     1261 tttgataaga taccaaatct tcccgtactt tcagcagaaa ctttagatca actgttgcct
     1321 tggaacaa
//
LOCUS       DACGLE010000170         1261 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0534
            SAMEA3304449-rid9560453.denovo.182, whole genome shotgun sequence.
ACCESSION   DACGLE010000170 DACGLE010000000
VERSION     DACGLE010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304449
            Sequence Read Archive: ERR985064
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1261)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1261)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702225.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702225.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 20:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,045
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 27 of 67
            Pseudo Genes (incomplete)         :: 25 of 67
            Pseudo Genes (internal stop)      :: 19 of 67
            Pseudo Genes (multiple problems)  :: 4 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1261
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304449-rid9560453.denovo.182"
                     /strain="VREN0534"
                     /db_xref="taxon:1352"
     gene            complement(<1..1103)
                     /locus_tag="IXT20_002846"
     rRNA            complement(<1..1103)
                     /locus_tag="IXT20_002846"
                     /product="23S ribosomal RNA"
BASE COUNT          278 a          357 c          243 g          383 t
ORIGIN      
        1 cctagttgtc tgtgcaaccc cacatccttt tccacttaac atatattttg ggaccttagc
       61 tggtggtctg ggctgtttcc ctttcgacta tggatcttat cactcacagt ctgactcccg
      121 gatatgaatg aatggcattc ggagtttatc tgaattcggt aacccgagat gggcccctag
      181 tccaaacagt gctctacctc catcattctc aattccgagg ctagccctaa agctatttcg
      241 gagagaacca gctatctcca agttcgtttg gaatttctcc gctacccaca cctcatcccc
      301 gcacttttca acgtacgtgg gttcggtcct ccagtgcgtt ttaccgcacc ttcaacctgg
      361 acatgggtag atcacatggt ttcgggtcta cgactacata ctcaaacgcc ctattcagac
      421 tcgctttcgc tgcggctccg tctcttcaac ttaacctcgc atgcaatcgt aactcgccgg
      481 ttcattctac aaaaggcacg ccatcaccca ttaacgggct ttgacttgtt gtaggcacac
      541 ggtttcaggt tctatttcac tccccttccg gggtgctttt cacctttccc tcacggtact
      601 ggttcactat cggtcactag ggagtattta gccttgggag atggtcctcc cggattccga
      661 cggaatttct cgtgttccgc cgtactcagg atcctcctag gtgtcttcca catttcgtct
      721 acggggtttt taccctcttt gactgacttt tccaagtcat tcgactatct gaaagaacta
      781 ccatattgga gtcctacaac cccaacaagc aagcttgctg gtttgggctt ttcccgtttc
      841 gctcgccgct actcagggaa tcgaattttc tttctcttcc tgcaggtact tagatgtttc
      901 agttctctgc gtctacctcg aatgtgctat gtattcacac aatcgtaaca tcctattaaa
      961 gatgttgggt tcccccattc ggaaatctct ggatcatagc ttacgtacag ctccccaaag
     1021 catatcggtg ttagtcccgt ccttcatcgg ctcctagtgc caaggcatcc accgtgcgcc
     1081 cttattcact taaccttatc aaccttgcgg ttgggttttg atccttcttc tagcgataga
     1141 agttagatca agaaaataag caattgaact tattaaaaaa ctctatccag ctcacaaatc
     1201 agcctgtgcg gcaaaaagat cgatcgtctc ataaaatcaa agattttata gccgtccgcc
     1261 t
//
LOCUS       DACGLE010000171         1261 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0534
            SAMEA3304449-rid9560453.denovo.183, whole genome shotgun sequence.
ACCESSION   DACGLE010000171 DACGLE010000000
VERSION     DACGLE010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304449
            Sequence Read Archive: ERR985064
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1261)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1261)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702225.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702225.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 20:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,045
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 27 of 67
            Pseudo Genes (incomplete)         :: 25 of 67
            Pseudo Genes (internal stop)      :: 19 of 67
            Pseudo Genes (multiple problems)  :: 4 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1261
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304449-rid9560453.denovo.183"
                     /strain="VREN0534"
                     /db_xref="taxon:1352"
     gene            complement(74..1222)
                     /gene="tnpB"
                     /locus_tag="IXT20_002847"
     CDS             complement(74..1222)
                     /gene="tnpB"
                     /locus_tag="IXT20_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family element transposase accessory
                     protein TnpB"
                     /protein_id="HAQ4861220.1"
                     /translation="MEQLKAYKFRIYPTEEQEIFFAKSFGCVRKVYNLMLDDRKKAYE
                     EVKNDSSKKMTFPTPAKYKKEFPFLKEIDSLALANAQLNLDKAYKNFFRDKSVGFPRF
                     KSKKNPVQSYTTNNQNGTVALIDSKFIKVPKLKSLVRIKLHRQPKGIIKSATISRHSS
                     GKYYISLLCKEEVRELPKSNSAVGIDLGIIDFAILSTGQKFDNNKFTSKMEKKLKREQ
                     RKLSRRALLAKQKGINLFEARNYQTQKRKVARLHEKVMNQRTDFLNKLSTEIIKNHDI
                     ICIEDLNTKGMLRNHKLAKSISDVSWSSFVSKLQYKADWYGRKIIKVDKWFPSSQICS
                     NCGHKDGKKSLEIREWTCPICHTHHDRDINASINILTEGLRLHSMGLA"
BASE COUNT          359 a          222 c          208 g          472 t
ORIGIN      
        1 ttactttact aacagaggtg cctcttattt accaagctat ccccgtagtt cctacggttc
       61 ttgtttattt gatctaagcc aatcccatag agtgtagcct tagaccttcg gtcaggatat
      121 tgatactagc gttgatatct cggtcatgat gagtatgaca aatagggcaa gtccattctc
      181 gaatttcgag agatttcttg ccgtctttat gtccacaatt cgaacagatt tggctagatg
      241 gaaaccactt atctactttg atgattttcc gaccgtacca gtcagccttg tattgtaatt
      301 tagacacaaa actagaccaa gaaacatccg aaatactttt tgctagttta tgattacgca
      361 acatgccttt tgtgtttaag tcttcaatac agatgatatc gtgatttttg ataatttctg
      421 tactcaactt attcagaaaa tcagtacgtt gattcattac tttttcatgc aatcgtgcta
      481 ctttacgttt ttgtgtttga taatttctag cttcaaacag attgatacct ttttgtttag
      541 ctaataaggc acgtcttgac aacttacgtt gttcacgttt gagtttcttt tccattttgg
      601 acgtaaattt attattatca aatttttgac cggtagaaag aattgcaaag tctataatac
      661 ctaggtcaat accaacagca gaattagatt taggtagttc gcgaacttct tctttgcata
      721 acaaagaaat ataatattta ccgctagaat gacgtgatat tgtagcggat ttgataatcc
      781 cttttggttg tctatgaagc ttaattctta ctaatgactt caatttagga actttgatga
      841 atttactatc aatcaaagca actgtaccat tttgattatt cgttgtataa ctctgaacag
      901 gatttttctt acttttgaaa cgtggaaatc caacggattt atcccgaaaa aaattcttgt
      961 atgctttatc taaattaagt tgggcattgg ctaaagcaag gctatcaatt tctttcaaaa
     1021 acggaaattc tttcttgtat ttagctggtg tcggaaaagt cattttttta gaagaatcat
     1081 ttttaacttc ttcatacgcc ttttttcggt catcaagcat tagattgtag accttacgga
     1141 cacaaccaaa agatttggca aagaagattt cttgttcctc tgttggatag attctgaatt
     1201 tgtatgcttt tagttgttcc ataaagttca cctactttct atttatgatt gtttccttga
     1261 t
//
LOCUS       DACGLE010000172         1234 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0534
            SAMEA3304449-rid9560453.denovo.184, whole genome shotgun sequence.
ACCESSION   DACGLE010000172 DACGLE010000000
VERSION     DACGLE010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304449
            Sequence Read Archive: ERR985064
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1234)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1234)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702225.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702225.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 20:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,045
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 27 of 67
            Pseudo Genes (incomplete)         :: 25 of 67
            Pseudo Genes (internal stop)      :: 19 of 67
            Pseudo Genes (multiple problems)  :: 4 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1234
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304449-rid9560453.denovo.184"
                     /strain="VREN0534"
                     /db_xref="taxon:1352"
     gene            <1..208
                     /locus_tag="IXT20_002848"
     CDS             <1..208
                     /locus_tag="IXT20_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009320234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HAQ4861221.1"
                     /translation="TNSYQAAILTAVNLGGDTDTTAAVTGALAGILYGGEVIPENWRK
                     SLQNDQLLKTISQRFCDTVLSQRI"
     gene            277..543
                     /locus_tag="IXT20_002849"
     CDS             277..543
                     /locus_tag="IXT20_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002383791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HAQ4861222.1"
                     /translation="MLVQMMNGGRIMSQSTITFRIPDDEKELVLEYAKAHNSSLTELY
                     RNAVLDKIEDEIDLRTLRSAIEVSKEKKESGISQEEMEKLLNEL"
     gene            533..802
                     /locus_tag="IXT20_002850"
     CDS             533..802
                     /locus_tag="IXT20_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HAQ4861223.1"
                     /translation="MNYKVIYMPQAQKQLKKMDRNQAKIIVAWIRKNLENTTDPRQHG
                     KGLTGNKSGEWRYRIGNYRILANICDSEIEIQIFSIGHRSTIYKR"
BASE COUNT          404 a          225 c          277 g          328 t
ORIGIN      
        1 tacgaatagt tatcaagcag ccatattgac tgcggtcaat ttaggagggg atactgatac
       61 tactgctgcc gtaactggtg ctcttgcggg tattctttac gggggtgaag tgatccctga
      121 aaattggaga aaatcgcttc aaaatgacca gcttcttaaa acgatcagtc agagattttg
      181 cgataccgtg ctatctcaaa gaatttgaat aatggatatg atgcaaatct tgtttgtact
      241 aacgttgtat tgacaaaaag gattttattt agtacaatgt tagtacaaat gatgaacgga
      301 ggtagaatca tgtctcaatc aaccattaca tttaggattc ccgacgatga aaaagaactg
      361 gtattagaat atgcaaaagc acacaattct tcattgaccg aactttatcg aaatgctgta
      421 ctggataaaa tcgaagatga aatcgatcta cggactttac ggagtgcaat tgaagtttcc
      481 aaagagaaaa aagagagtgg tatttctcaa gaggaaatgg agaaactgct aaatgaatta
      541 taaagtcatc tacatgccgc aagcccagaa gcaattaaaa aaaatggaca gaaaccaagc
      601 taaaataatt gtcgcctgga tcagaaagaa tttagaaaac acgactgacc cgcgtcagca
      661 cggaaaaggt ttgactggaa ataagagtgg ggaatggcgc tatcgaattg gaaactatcg
      721 aatattggct aatatttgtg attctgaaat tgaaatccag atattctcaa ttggacatag
      781 aagtacaata tataaaagat aaccgttttt ggaacgaagt agagtttcga aagtcaatgt
      841 ataatctttt gaattttgca taataccaca ggtagaaact acccgatact atcggttggt
      901 ttctgctttt ttatttggtc ttgaagaaga aaacgagtga tctgtggcaa agaaagaaac
      961 aaaaaaagga caaaactgtt gcttcgcctg cggtgcagtc gcccttggat caaagcgcgt
     1021 ctgttttctt ttggctagtg gcaaggtcta actgcttggt ggctacgtgc cttcgccagt
     1081 cgcagctgcc ttggttggct gcgtgcctcc gccaagtcgg ccgacctcac catttttgcc
     1141 tacgcaaaaa ggccaaaagg atttaaagga atccgtcgaa gcatagctcc aacttctccg
     1201 gatcaaaaag cggtaggaac ccgtgccggg ttcc
//
LOCUS       DACGLE010000173         1215 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0534
            SAMEA3304449-rid9560453.denovo.185, whole genome shotgun sequence.
ACCESSION   DACGLE010000173 DACGLE010000000
VERSION     DACGLE010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304449
            Sequence Read Archive: ERR985064
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1215)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1215)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702225.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702225.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 20:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,045
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 27 of 67
            Pseudo Genes (incomplete)         :: 25 of 67
            Pseudo Genes (internal stop)      :: 19 of 67
            Pseudo Genes (multiple problems)  :: 4 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1215
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304449-rid9560453.denovo.185"
                     /strain="VREN0534"
                     /db_xref="taxon:1352"
     gene            414..947
                     /locus_tag="IXT20_002851"
     CDS             414..947
                     /locus_tag="IXT20_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease"
                     /protein_id="HAQ4861224.1"
                     /translation="MNFVAMDFETANHQPYSACSLALVMVKNSQIVDEFYTLIQPETP
                     FFWRNVQIHGIHQEDVRNAPKFPEVWQTIQPYFQKNRLVVAHNAAFDTKVLAGCLDYY
                     HLEQPNYLSLCTVKSSRRLFPEFPNHRLNTVCENLNIQLDHHHDALEDSRACAEILLY
                     QEKKFGTDPLKKLVTIK"
BASE COUNT          389 a          226 c          225 g          375 t
ORIGIN      
        1 cttattaccg cagatgcaag cttttgaaca ccgtttattg agaagttgtg aaatcagcgt
       61 cctgacctga gtattagaga aacaaatggc aaaatagttc ctcatatttt gctatttttt
      121 gagctaatac cgagggtcag ctgattgaac accgtttagt ggagaagttg tgaaaaagtc
      181 gcctactttc gagtcgtaag aaattttttt gaaaatggtt cctcacaact ttgcataaat
      241 ttgacttaat accgagaagt catgatcccc atctaactcc aaatcacaag gaatgagttc
      301 aaaaactagc ttttctcaac aaatattaca taaaaaattc tatgtttttt gttaaattta
      361 tttgcttttt tcaaaagtat gttacagtaa tttcccaagg aggttttaca gaaatgaatt
      421 ttgttgccat ggattttgaa actgccaatc atcagcctta tagtgcttgt tctcttgcgc
      481 tagtcatggt aaaaaatagc cagatcgttg atgaattcta tacattgata cagcctgaga
      541 ctcctttttt ctggcgaaat gtccaaattc acggaatcca tcaagaagac gtacgcaatg
      601 cgccgaaatt cccagaagtc tggcaaacta tccaaccata ttttcaaaaa aatcgtttag
      661 tcgttgcaca taatgctgct tttgatacaa aggtcttagc gggatgctta gattattatc
      721 atcttgaaca gcctaattat ctttctttat gtacagtgaa aagtagccgt cgcctgtttc
      781 ctgaatttcc aaatcatcga ttgaacactg tgtgcgaaaa tttgaacatc caattagatc
      841 atcaccatga tgcattggaa gatagtcgtg catgtgcgga aatcctgctt tatcaagaaa
      901 aaaaattcgg gacagatcca ttgaaaaaac ttgtgactat aaagtaaaag aatacttatg
      961 aaacacacaa agaggtcgtg agcctgccgt ttcactccga gtcacaagga gcaatttcaa
     1021 aaaatagctt ctcatgttta ttaaatattg tgactggtga ccgaggagct ggcaggagaa
     1081 caccgtttat tggagaggtt gtgaaaaagc tgttctgcat caagtaataa gaacagccaa
     1141 acaaaaatag cttctcatat ttttcatttg gttggactta ttaccgcaga tgcaagcttt
     1201 tggacaccgt ttacc
//
LOCUS       DACGLE010000174         1193 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0534
            SAMEA3304449-rid9560453.denovo.186, whole genome shotgun sequence.
ACCESSION   DACGLE010000174 DACGLE010000000
VERSION     DACGLE010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304449
            Sequence Read Archive: ERR985064
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1193)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1193)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702225.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702225.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 20:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,045
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 27 of 67
            Pseudo Genes (incomplete)         :: 25 of 67
            Pseudo Genes (internal stop)      :: 19 of 67
            Pseudo Genes (multiple problems)  :: 4 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1193
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304449-rid9560453.denovo.186"
                     /strain="VREN0534"
                     /db_xref="taxon:1352"
     gene            <1..1076
                     /locus_tag="IXT20_002852"
     CDS             <1..1076
                     /locus_tag="IXT20_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HAQ4861225.1"
                     /translation="VIGMESTSMYSFHPSMFFHEDEKLKKLNTLVTIENPFRVKQFSR
                     MFDENKTDRNDALRIADFLRIQRFTTSPIKEEKYMALQRLTRTRYQLIKQLIRTKQHF
                     LENLTYKCNTLAREMRDESTSLFSATLISLMTEDFTLDELAELPLEAFCDLLQEKGKG
                     RFKQPEKIAKAIQRAITMSYRLGALAQESINVVLSVLVREIRALEKNIKELDKAIEQV
                     IVVIPEYQCLTSIPGVGKVYAAGLIAEIGQIERFEDQTKLAKYAGLSWKVNQSGNYQS
                     QNTPLTKQGNRYFRYYLVEAANSVKNYLPEYKAFYQSKYKEVPKHQHKRALVLTARKF
                     TRLVDTLLRKHQLYTPPRSVIEK"
BASE COUNT          418 a          234 c          210 g          331 t
ORIGIN      
        1 ttgtgattgg catggagtca acgtccatgt acagcttcca tccttccatg ttttttcatg
       61 aagatgagaa actaaagaag ctcaacacac ttgtaacgat tgaaaatcct tttcgggtga
      121 aacaatttag tcgcatgttt gatgagaata aaactgatcg aaacgatgca ttaagaatcg
      181 ctgatttctt gcgtattcaa cgattcacaa cttcccctat caaagaagag aaatacatgg
      241 ccttacagcg tttaacacgt actcgctatc aacttattaa gcaattgatt cgtacaaagc
      301 aacattttct tgaaaatcta acgtacaaat gtaatacgct tgctcgagaa atgcgagacg
      361 aatcgacgag tctttttagt gcaaccctca tttcactcat gacggaagat ttcacattgg
      421 atgaacttgc tgagcttcct ttagaagctt tctgtgattt acttcaagaa aaaggaaaag
      481 gccgtttcaa gcaaccagaa aaaatcgcta aagcaattca acgtgcaatt accatgagtt
      541 accgcctagg tgctcttgca caagaatcaa ttaatgttgt tttaagtgtt ctcgtacgag
      601 aaatacgagc acttgaaaaa aatatcaaag aattagataa agccatcgaa caagtaattg
      661 ttgtcattcc agaatatcaa tgcttaacca gtattccagg cgttggtaaa gtctatgctg
      721 ccggtttaat cgctgaaatt ggtcaaatcg aaagatttga agatcagaca aaattggcaa
      781 aatacgctgg attaagttgg aaagtgaacc aatctggaaa ctatcagtcg caaaatacac
      841 ctctgacaaa acaaggaaat cgatatttta gatactactt agttgaagct gccaactctg
      901 taaaaaatta tcttcctgaa tacaaagctt tttatcaaag taaatataag gaggttccaa
      961 agcatcaaca caaacgtgca ctcgtcctaa ccgcaagaaa atttacgcgt ctggtggata
     1021 cgctactacg taaacaccaa ctctatacgc caccaaggag tgtgatagag aaataacaat
     1081 ccgttattga cgcaaatcct tgaatcaacc cgaaaaaaat ttgattttga tcgggtctag
     1141 tttcgtgcgc ttcaaaacat attactcaat aaagaaaatc ttaaatttca tct
//
LOCUS       DACGLE010000175         1188 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0534
            SAMEA3304449-rid9560453.denovo.187, whole genome shotgun sequence.
ACCESSION   DACGLE010000175 DACGLE010000000
VERSION     DACGLE010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304449
            Sequence Read Archive: ERR985064
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1188)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1188)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702225.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702225.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 20:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,045
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 27 of 67
            Pseudo Genes (incomplete)         :: 25 of 67
            Pseudo Genes (internal stop)      :: 19 of 67
            Pseudo Genes (multiple problems)  :: 4 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1188
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304449-rid9560453.denovo.187"
                     /strain="VREN0534"
                     /db_xref="taxon:1352"
     gene            141..398
                     /locus_tag="IXT20_002853"
     CDS             141..398
                     /locus_tag="IXT20_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4861226.1"
                     /translation="MSNLENKEEKVVNKIVSVVNKLDKELDELDTLSENPEKKHNLKK
                     WLVERKAIHEIKKVLHEADKYEKYDEKELDKEFKEINDLLL"
     gene            complement(630..1043)
                     /locus_tag="IXT20_002854"
     CDS             complement(630..1043)
                     /locus_tag="IXT20_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014834562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4231 domain-containing protein"
                     /protein_id="HAQ4861227.1"
                     /translation="MDQKAFIESIDSTIDRLKKEIRFYNRVIGIGNIIKIVLSASIPI
                     LIDQASEHRSLLLIVSIASAIITIIQSGMSAFNYQDKVQTSTELLMKIEKEKLLYITK
                     TSPYNKTDEENFHLIVTTLQTELNDIISDFNQVNN"
BASE COUNT          453 a          151 c          148 g          436 t
ORIGIN      
        1 ttctgtccac gttcatttta ctccaaagtt ttcctcattt caaaagaaaa tcacagaata
       61 cacgaagaag cgtttctatt tgtataaact atatcaaagt tctatactta aattatcaaa
      121 taaaaaggac gtgaagataa atgtcaaatt tagaaaacaa agaagaaaaa gttgtaaaca
      181 aaattgtttc tgttgtcaat aaactggata aagagttaga cgaacttgat acgttatctg
      241 aaaatccaga aaaaaaacat aacctaaaaa aatggttagt tgaacgaaag gctattcatg
      301 aaattaaaaa agttcttcat gaagctgata aatatgaaaa atatgatgaa aaagaacttg
      361 ataaagaatt taaagaaatc aatgacttat tactataatt gatttatacg actcattttt
      421 gagtcgtcag actgcaaaca acactctaaa tttaggagtt tgtctacagt ctgaataaat
      481 tttaaattta ttgtccataa tgttattatg aatttatagc attaagaaaa gagataagac
      541 tttacatttt tttaggaatg taaagtctta tctcttttct taattgtaca attagtatta
      601 tctgcactac tttctaatta aataaatttt tagttattaa cttgattaaa atcagagata
      661 atatcattta attcagtttg aagggttgta acgattaaat ggaaattttc ttcatctgtt
      721 ttattatatg gggatgtttt cgttatatat aacaactttt ctttttcaat tttcattaat
      781 aactctgtcg aagtctgtac tttatcttga taattaaatg cagacatacc actttgtata
      841 attgtaatta ttgctgaagc aattgaaaca atcaatagta aagacctgtg ttcggaagcc
      901 tgatcaatta atattgggat actagctgat aagactattt ttataatatt tccgatacca
      961 attactctat tataaaatcg aatttctttt tttaatctat cgattgtaga atcaatagat
     1021 tcgataaacg ctttttgatc catcattttc tctccaatca ttttattaaa atcattaaca
     1081 aaaatattat aataggattt aagtaagttc taactaatca catataaaaa taaaaaaaca
     1141 tttgtatttt atcaaagttt tattatactt gtcaatttgt gtacatcc
//
LOCUS       DACGLE010000176         1115 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0534
            SAMEA3304449-rid9560453.denovo.188, whole genome shotgun sequence.
ACCESSION   DACGLE010000176 DACGLE010000000
VERSION     DACGLE010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304449
            Sequence Read Archive: ERR985064
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1115)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1115)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702225.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702225.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 20:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,045
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 27 of 67
            Pseudo Genes (incomplete)         :: 25 of 67
            Pseudo Genes (internal stop)      :: 19 of 67
            Pseudo Genes (multiple problems)  :: 4 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1115
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304449-rid9560453.denovo.188"
                     /strain="VREN0534"
                     /db_xref="taxon:1352"
     gene            30..1004
                     /locus_tag="IXT20_002855"
     CDS             30..1004
                     /locus_tag="IXT20_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase family protein"
                     /protein_id="HAQ4861228.1"
                     /translation="MCTSITYVTSDHYFGRNFDYEISYNEVVTVTPRNYKLNFRKVND
                     LDTHYAMIGIAAGIADYPLYYDATNEKGLSMAGLNFSGYADYKEIQEGKDNVSPFEFI
                     PWILGQCSTVGETKKLLKNINLANINYSDELPLSPLHWLLADKEKSIVIESMKDGLHI
                     YDNPVGVLTNNPSFDYQLFNLNNYRVLSSETPKNNFSNQISLNAYSRGMGGIGLPGDL
                     SSVSRFVKATFTKLNSVSGDSESESISQFFHILGSVEQQKGLCDVGDGKYEYTIYSSC
                     CNVDKGIYYYRTYEDSQITAIDMNKEDLDSHKLISYPIIEKQQIKYIN"
BASE COUNT          394 a          136 c          193 g          392 t
ORIGIN      
        1 aaatgatatt ttacgaggag gaataaacta tgtgtacgtc tattacttat gtaacaagtg
       61 atcattattt tggaaggaat tttgattatg aaatatctta caatgaagta gttactgtta
      121 ctccaagaaa ttataagttg aattttcgaa aggtaaatga tttggatact cattatgcaa
      181 tgattggtat tgccgctggt atagctgact accctcttta ttacgatgcg acaaatgaaa
      241 aaggattgag tatggctggg ctaaattttt ctgggtatgc tgattataaa gaaatacaag
      301 aagggaaaga caatgtatct ccttttgaat ttattccttg gattttagga caatgctcaa
      361 cagtaggaga aactaaaaaa ttgttaaaaa atatcaattt agcaaatata aattatagtg
      421 atgaacttcc tttatcccct ttacattggc tattagctga taaagaaaaa tcaattgtca
      481 ttgaaagtat gaaagatgga cttcatatat atgataaccc tgtgggcgtt cttaccaata
      541 atccttcatt tgactatcaa ttatttaatt taaacaatta tcgtgtctta tcgagtgaaa
      601 ctcctaaaaa taatttttca aatcaaataa gtttgaatgc ctatagccgc ggtatgggag
      661 ggataggctt gcctggagat ttatcctcag tatctcgttt tgttaaagcg acttttacga
      721 agctgaattc tgtatctgga gattcagagt cagaaagtat tagtcaattt ttccatatct
      781 taggttcagt agaacaacaa aaaggtttgt gtgatgttgg tgatggaaaa tatgaatata
      841 caatttattc ttcttgttgc aatgttgaca aaggaatcta ttattatcga acatatgaag
      901 acagtcaaat tactgcaatt gatatgaata aagaagactt agatagtcat aagttaatta
      961 gttatccaat tatagaaaaa caacaaatta aatatataaa ttagttaatg tgttgtgatt
     1021 gattatttaa tatagatata atacaaaaag gcaaagatat tttcaaaaca ttttttcttg
     1081 atgtgatatc cttgtctttt ttgtaatgtt tttaa
//
LOCUS       DACGLE010000177         1091 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0534
            SAMEA3304449-rid9560453.denovo.189, whole genome shotgun sequence.
ACCESSION   DACGLE010000177 DACGLE010000000
VERSION     DACGLE010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304449
            Sequence Read Archive: ERR985064
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1091)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1091)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702225.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702225.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 20:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,045
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 27 of 67
            Pseudo Genes (incomplete)         :: 25 of 67
            Pseudo Genes (internal stop)      :: 19 of 67
            Pseudo Genes (multiple problems)  :: 4 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1091
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304449-rid9560453.denovo.189"
                     /strain="VREN0534"
                     /db_xref="taxon:1352"
     gene            complement(<1..126)
                     /locus_tag="IXT20_002856"
     CDS             complement(<1..126)
                     /locus_tag="IXT20_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016178545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ4861229.1"
                     /translation="MIQELNRLMTYIEARLTKEISLAEAAKTMGLSEYHLKRTFSF"
     gene            700..>1091
                     /locus_tag="IXT20_002857"
     CDS             700..>1091
                     /locus_tag="IXT20_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002425010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4861230.1"
                     /translation="MIGLLSSTATITTPVSVLTGFSIGFVSTGLFGTVGLFGSVGLLG
                     SVGLFGSVGLLGSVGLPGSVGSVGSVGSVGSVGSLGSVGSLGSVGSLGSVGSLGSTGS
                     TGSTGSTGSTGSTGSTGSTGSTGSTGSTG"
BASE COUNT          245 a          223 c          212 g          411 t
ORIGIN      
        1 aaaagagaac gtacgcttta aatggtattc agataatccc atcgttttag cagcctctgc
       61 cagtgaaatc tcttttgtta atcgtgcttc gatatacgtc atcaaccgat tcaactcttg
      121 aatcatttct tccacctcac ctatgtactt attctatata agattccgtt attggacttg
      181 ataaatgcta gactaatatt atcggttgca tcctttggaa tgacaaatca tttaaacttg
      241 attttcatgc acgtactcct aaaaaaataa tgactttttc cgactcattt gtcttcaaca
      301 actattaaac gtctctgtac gcacaattgc taacagaata ctcgataggt tctgttgtta
      361 ttggtgttgc ctagaacgat tctaagcgct tgtttaattt taaggtatac attccccttc
      421 aaaacgtcct agaatgattc aataaaagca tggctgctag tgaaacttgc gaccacgctt
      481 ttataaatac ttaattagat tcgcttttta aaaatgaatg ctgatcctaa taccattact
      541 gaccctagga cagacaatgc aatattttct gtgtctcctg tttttggtaa aactttcatt
      601 tttccgtctg aacctttaac cgttactgtt ccgtcttttt gaacgtttgc accaatttct
      661 ttagcttcaa ctttttttgt tgttccgtct gctaattgaa taatcggttt actatcttca
      721 actgcaacaa tcacaacgcc tgtatctgta ttaactgggt tttcaattgg gtttgtgtct
      781 actggtttgt ttggtactgt tggtttgttt ggctctgttg gtttacttgg ttctgtcggc
      841 ttgtttggct ctgttggttt acttggttct gtcggcttgc ctggttccgt tggttccgtt
      901 ggttccgttg gctccgttgg ttctgttggt tcgcttggct ctgttggttc gcttggttct
      961 gttggttcgc ttggctctgt tggttcgctt ggctcgactg gatcaactgg atcaactgga
     1021 tcgactggat cgactggatc aactggatcg actggatcaa ctggatcaac tggatcgact
     1081 ggatcaactg g
//
LOCUS       DACGLE010000178         1058 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0534
            SAMEA3304449-rid9560453.denovo.190, whole genome shotgun sequence.
ACCESSION   DACGLE010000178 DACGLE010000000
VERSION     DACGLE010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304449
            Sequence Read Archive: ERR985064
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1058)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1058)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702225.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702225.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 20:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,045
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 27 of 67
            Pseudo Genes (incomplete)         :: 25 of 67
            Pseudo Genes (internal stop)      :: 19 of 67
            Pseudo Genes (multiple problems)  :: 4 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1058
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304449-rid9560453.denovo.190"
                     /strain="VREN0534"
                     /db_xref="taxon:1352"
     gene            complement(36..992)
                     /locus_tag="IXT20_002858"
     CDS             complement(36..992)
                     /locus_tag="IXT20_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4861231.1"
                     /translation="MELYTLPIIIKTEKMLKDLKQYYPLSAYEKKKLFHFLAINLLAE
                     QADSLDIASNQSLFFSDIGFYHLKGVLGEKSVIFINKTLSWLYLHDKLDQKYLRKNED
                     ETIEWLNERFIESFENQFNLLREETSQLIRRELTKIHFKLLHYTINKADEFPLDVSFF
                     RENYPAFYCYLIEYINDLTSKRKELLQDKFFLFFSYLLLLINHIPIQLISEPVKIIID
                     FSYGAAYNQFIKKNLSLYINLNTEVIEAGKPEFPDVIITNRNNLYEESASQVVVWLDP
                     PRAVDWGNLTKILLTIQEEKYKDSKIDKQIDDFPEEIIDRIE"
BASE COUNT          344 a          176 c          160 g          378 t
ORIGIN      
        1 tagaaaaagc agtcggactt tctaaaagaa gttcactatt cgatcctgtc gatgatttct
       61 tcaggaaaat cgtctatttg cttgtcaatt ttgctgtctt tgtatttctc ttcttggata
      121 gtcaacaaaa tctttgtaag attcccccag tctactgctc ttggcggatc gagccaaacc
      181 actacttgag atgcactttc ttcatataga ttattcctat tcgtgattat aacatccggg
      241 aactcaggct ttcctgcctc tatcacttcc gtgttcagat taatgtacaa gcttaaattt
      301 ttcttgataa attgattata ggcagcacca taagaaaaat cgatgatgat ctttactggt
      361 tcagaaatca gttgaatggg tatgtgattg atcaataata ataaataact gaaaaaaaga
      421 aaaaatttat cttgaagcaa ctctttgcgt tttgacgtta gatcatttat atactctatc
      481 aaatagcaat aaaatgctgg atagttttct ctaaaaaaag aaacatcaag tggaaattca
      541 tcagctttat taatagtgta atgcaaaagt ttaaaatgta ttttggttaa ctctcttcgg
      601 atcaattggc tcgtctcttc tcgcaacaga ttaaactgat tttcaaaact ttcaatgaat
      661 cgttcattca accattcgat tgtttcatct tcattttttc tcaagtattt ttgatccaat
      721 ttatcatgaa gataaagcca agagagtgtc ttattgataa aaataacgga tttttctcct
      781 aatactcctt ttaagtggta aaatcctata tccgaaaaaa atagcgactg attgctagca
      841 atatctaaag aatcagcttg ttcagccaac aggttgattg ctaaaaagtg gaataatttc
      901 tttttttcat aagcagacag aggataatac tgttttaaat cttttaacat tttttctgtt
      961 tttataatta taggtagcgt atacaattcc acaaaactta tctctgttga gttaaaaaga
     1021 ttatataaaa aaagccgaat gtttttagga ttccctgt
//
LOCUS       DACGLE010000179          973 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0534
            SAMEA3304449-rid9560453.denovo.192, whole genome shotgun sequence.
ACCESSION   DACGLE010000179 DACGLE010000000
VERSION     DACGLE010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304449
            Sequence Read Archive: ERR985064
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 973)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 973)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702225.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702225.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 20:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,045
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 27 of 67
            Pseudo Genes (incomplete)         :: 25 of 67
            Pseudo Genes (internal stop)      :: 19 of 67
            Pseudo Genes (multiple problems)  :: 4 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..973
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304449-rid9560453.denovo.192"
                     /strain="VREN0534"
                     /db_xref="taxon:1352"
     gene            35..652
                     /locus_tag="IXT20_002859"
     CDS             35..652
                     /locus_tag="IXT20_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAQ4861232.1"
                     /translation="MAKIGYARVSSLDQNLDRQLELLSECDKIFSDKASGKDTNREGF
                     QEMMKFIREGDIVVVTELKRLGRNNKELTETMNLIQLKKATLEVLNLPALTGITDDNL
                     RRLLNNLIIELYKYQAEEERRYIRETQQQGIALAKAKGKYKGGTLKYRENDPRLQLAF
                     NLFLTGSTDKEVEELTGINRRTFRRYRMKHGITNEVREQKNKSFS"
BASE COUNT          345 a          128 c          196 g          304 t
ORIGIN      
        1 gatccggatt tcagaatttt aaaggagttt tattatggcg aaaattgggt atgctcgagt
       61 aagcagctta gatcaaaatt tagaccgaca attagaatta ttatcagagt gtgataaaat
      121 ttttagtgat aaagcaagtg ggaaagatac caatcgagaa ggatttcaag aaatgatgaa
      181 attcatacgt gaaggtgata ttgtcgttgt gacagaatta aaacgacttg gacgaaataa
      241 taaagagcta acagaaacga tgaatttaat tcagctgaag aaagctacac tggaagtttt
      301 gaatttgcca gcgttaacag gtatcactga tgataattta cgtcgattac ttaataactt
      361 aattatcgaa ctatacaagt accaggccga agaagaacgt cggtatattc gcgaaacgca
      421 acaacaagga attgcactcg ctaaagcaaa agggaaatac aagggaggta cgctaaaata
      481 tcgtgagaat gatccgcgac ttcaattagc atttaatttg tttttgactg gatctacaga
      541 taaagaagtt gaagagctaa cagggattaa tcgtagaacg tttagaagat atcgaatgaa
      601 acatggtatt actaatgaag ttagggagca aaaaaataaa tctttcagtt gaggctaata
      661 taattttgca agaaaccagg tcttaagaaa gctcgtaaag cttcacaatt ctcaaaacgt
      721 taatcgttgt tatatcaagg atttcacggc atcatccgtt tggatggtgt cttttttggg
      781 ttgaattcct tgaaaattgg gtctatttta cagacccttt tctatataaa ctgttttcga
      841 tacttggaaa aatctgttgt ttttaaattt tatttaattc taatgtttac tatgttttta
      901 gcagtgttag gtaatgtaac tgcaataaat ttgttataca ataatggatt ttgattttgg
      961 gacgtaaaac att
//
LOCUS       DACGLE010000180          959 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0534
            SAMEA3304449-rid9560453.denovo.193, whole genome shotgun sequence.
ACCESSION   DACGLE010000180 DACGLE010000000
VERSION     DACGLE010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304449
            Sequence Read Archive: ERR985064
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 959)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 959)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702225.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702225.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 20:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,045
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 27 of 67
            Pseudo Genes (incomplete)         :: 25 of 67
            Pseudo Genes (internal stop)      :: 19 of 67
            Pseudo Genes (multiple problems)  :: 4 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..959
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304449-rid9560453.denovo.193"
                     /strain="VREN0534"
                     /db_xref="taxon:1352"
     gene            38..>959
                     /locus_tag="IXT20_002860"
     CDS             38..>959
                     /locus_tag="IXT20_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAQ4861233.1"
                     /translation="MTYNHLTPTELVMIEAYFNQSQPVSKVANLLQRSRQTIYKVYRF
                     LKSGGSAMSYYKQYKKNKRNCGRHPIVLPDEQQEYIQKKVVQGWAPDVLIGRAEFPIA
                     CSVRTLYRMFKDKTFDTHDLPMKGKRKPNGHKEKRGKQGFRRSIRDRKTDYTQFDHEF
                     GHLEGDTIVGGKHKSAVITLVERLSKVIITLKPEGRQAKDIENRLNQWFRCVPKNLFK
                     SITFDCGKEFSNWKKISNTNDISIYFADPGTPSQRGLNEHSNGLLRKDGLYKAMDFNG
                     VSETFIQSVASKRNHIPRKSLHYRTPMEVFL"
BASE COUNT          343 a          173 c          184 g          259 t
ORIGIN      
        1 atagttccga cgaaagaatt ataaggagtg agtacaaatg acctataacc atcttacacc
       61 aactgaactc gtaatgatag aagcttattt caatcaatca caacctgttt caaaagtagc
      121 caatctgtta caacgttctc gacaaactat ttataaggtc tatcgttttc ttaaatctgg
      181 tggaagcgca atgagctact ataaacaata taagaaaaac aaacgtaact gcggaagaca
      241 tccgattgtt ttacctgatg agcaacagga atacattcaa aaaaaggttg ttcaagggtg
      301 ggctcctgat gtccttattg gtcgagcaga gtttcctatt gcttgttcgg tacgcacact
      361 gtatcgaatg tttaaagata aaacctttga cactcacgat ttaccaatga agggaaaaag
      421 aaaaccaaac ggtcataaag aaaaaagagg aaaacaaggg tttcgccggt caatccgaga
      481 cagaaagaca gactataccc agtttgatca tgaatttggt cacctggaag gtgacactat
      541 cgtgggtgga aaacataaaa gcgcggtcat cacattagtt gaacggctat ctaaagtcat
      601 cattaccttg aaacccgaag gcagacaggc taaagacatt gaaaataggc tgaatcaatg
      661 gtttcgttgt gtcccaaaaa acttatttaa atccatcaca tttgattgtg gaaaagaatt
      721 ctcaaattgg aaaaagatta gtaataccaa cgatatttca atttattttg cggatccagg
      781 aacaccttct caacgaggat tgaatgaaca ttcaaatggc ttattacgta aagatggttt
      841 gtataaagct atggatttta atggtgtatc tgaaaccttt atccaatcgg tggcatcgaa
      901 acgaaatcat attcctagaa aatcattaca ctatcggaca ccaatggaag tatttttga
//
LOCUS       DACGLE010000181          949 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0534
            SAMEA3304449-rid9560453.denovo.194, whole genome shotgun sequence.
ACCESSION   DACGLE010000181 DACGLE010000000
VERSION     DACGLE010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304449
            Sequence Read Archive: ERR985064
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 949)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 949)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702225.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702225.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 20:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,045
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 27 of 67
            Pseudo Genes (incomplete)         :: 25 of 67
            Pseudo Genes (internal stop)      :: 19 of 67
            Pseudo Genes (multiple problems)  :: 4 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..949
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304449-rid9560453.denovo.194"
                     /strain="VREN0534"
                     /db_xref="taxon:1352"
     gene            28..>949
                     /locus_tag="IXT20_002861"
     CDS             28..>949
                     /locus_tag="IXT20_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAQ4861234.1"
                     /translation="MTYTHLTPTELVMIEAYFNQSHSVSKVAHLVQRSRQTIYNVYRF
                     LKSGGSAISYYEQYKKNKRNCGRRPIVLPDGQQEYIQKKVAQGWTPDVLIGRAEFPIA
                     CSVRTLYRMFKDKTFDPHDLPMKGKRKPNGHKEKRGKQAFRRSIQDRTTDYIQFNHEF
                     GHLEGDTIVGGKHKSAVITLVERLSKVIITLKPAGRQAIDIENRLNQWFQSVPKHLFK
                     SITFDCGKEFSNWKKISNTNDISIYFADPGTPSQRGLNEHSNGLLRKDGLYKAMDFNK
                     VSETFIQSVASKRNHIPRKSLHYRTPLEVFL"
BASE COUNT          335 a          175 c          180 g          259 t
ORIGIN      
        1 cgaaagaatt ataaggagtg agtacaaatg acctataccc atcttacacc aactgaactc
       61 gtaatgatag aagcttattt caatcaatca cactctgttt caaaagtagc ccatctggta
      121 caacgttctc gacaaaccat ttataatgtc tatcgttttc ttaaatctgg tggaagcgca
      181 ataagctact atgaacaata taagaaaaac aaacgtaact gcggaagacg tccgattgtg
      241 ttaccagatg ggcaacaaga atacattcaa aaaaaggtgg ctcaagggtg gactccagat
      301 gtccttattg gtcgagcaga gtttcctatt gcttgttcgg tacgtacact atatcggatg
      361 tttaaagata aaacctttga ccctcacgat ttaccaatga aagggaaaag aaaaccaaac
      421 ggacataaag aaaaaagagg aaaacaagct tttcgccgtt cgattcagga tagaacaact
      481 gattatatac agttcaatca tgagtttggt cacctagaag gtgacaccat tgtcggtgga
      541 aaacataaaa gtgcggtgat cacgttagtt gagcgattat ctaaagtgat catcaccttg
      601 aaacccgctg gtaggcaagc tatagatatt gagaatagac tgaatcaatg gtttcaatct
      661 gtaccgaaac acttgtttaa atccatcaca tttgattgtg gaaaagagtt ctcgaattgg
      721 aaaaagatta gtaataccaa tgatatttcg atttatttcg ctgatccagg aacaccttct
      781 caacgaggac taaatgaaca ttcgaatggc ttattacgta aagatggctt atataaagct
      841 atggatttta ataaggtatc cgaaaccttt atccaatcgg tggcatcaaa acgaaatcat
      901 attcctagaa aatcattaca ctatcgcaca ccattggaag tatttttga
//
LOCUS       DACGLE010000182          910 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0534
            SAMEA3304449-rid9560453.denovo.195, whole genome shotgun sequence.
ACCESSION   DACGLE010000182 DACGLE010000000
VERSION     DACGLE010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304449
            Sequence Read Archive: ERR985064
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 910)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 910)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702225.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702225.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 20:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,045
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 27 of 67
            Pseudo Genes (incomplete)         :: 25 of 67
            Pseudo Genes (internal stop)      :: 19 of 67
            Pseudo Genes (multiple problems)  :: 4 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..910
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304449-rid9560453.denovo.195"
                     /strain="VREN0534"
                     /db_xref="taxon:1352"
     gene            <1..878
                     /locus_tag="IXT20_002862"
     CDS             <1..878
                     /locus_tag="IXT20_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HAQ4861235.1"
                     /translation="SLNQPLPEVVCFDEFKSVKNVSGAMSFVMMDGKTHQLIDIVENR
                     QLNPLRDYFLRYPRKVREQVRLVVSDFYTPYRTLVKECFPNARIVADRFHISQHIGRA
                     FTNHRIQVMKTFKKRDRRSKHLKKYWRLLQKNAWELKGQHRYWRPSFRDHLTEAEIVD
                     RLLSYDDSLKRGYEVYQDFLSAIRRQDVPEFVALLKEDYKELPEHYQPVFTTFKKYRT
                     EIKRALRVPYSNGPIECLNNHIKVLKRIAYGFRNFQNYQERIFLYRGKYFKKTKNTTQ
                     LTKARTTTRLDKIAV"
BASE COUNT          291 a          202 c          184 g          233 t
ORIGIN      
        1 tttctttgaa ccagccgtta ccagaagtgg tgtgtttcga tgaattcaaa tcggtgaaaa
       61 acgtctcggg agccatgagc tttgtcatga tggacggcaa gacccatcaa ctgattgata
      121 ttgtggaaaa tcggcaacta aatcctttac gcgactattt cttgcgctac cctagaaaag
      181 tccgtgaaca agtacgtcta gtggtttcag atttctatac accctatcgc acattggtga
      241 aagagtgctt tccgaatgcc cgaatcgtcg ccgatcgctt tcatattagc caacacatcg
      301 gtcgggcctt taccaaccac cgaattcaag taatgaagac ctttaaaaaa agggatcgtc
      361 ggtcgaagca cttaaagaaa tactggagac tacttcaaaa gaatgcgtgg gaattgaaag
      421 gtcagcatcg ctactggcgt ccgtcatttc gagatcatct gacagaagca gagatagtcg
      481 atcggctatt atcttatgat gactccttaa agcgaggcta cgaagtctac caggattttc
      541 tttccgctat cagaagacaa gatgttcctg aattcgtcgc gctcctaaaa gaagactaca
      601 aggagttgcc agagcactac caaccagtgt tcacgacctt caagaaatat cgaacagaaa
      661 tcaaacgagc gttgcgagtt ccctattcca acggccctat agaatgtttg aacaaccata
      721 tcaaagtact aaaacgaatt gcctatggct ttagaaactt tcaaaactac caagaacgaa
      781 tctttttata tcgaggaaaa tattttaaga aaactaagaa cactacccaa ctgactaaag
      841 ctagaacaac aacacggcta gacaagatag ctgtctagcc gtgtgagtat ctcgattatt
      901 ctattgaatt
//
LOCUS       DACGLE010000183          867 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0534
            SAMEA3304449-rid9560453.denovo.196, whole genome shotgun sequence.
ACCESSION   DACGLE010000183 DACGLE010000000
VERSION     DACGLE010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304449
            Sequence Read Archive: ERR985064
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 867)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 867)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702225.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702225.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 20:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,045
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 27 of 67
            Pseudo Genes (incomplete)         :: 25 of 67
            Pseudo Genes (internal stop)      :: 19 of 67
            Pseudo Genes (multiple problems)  :: 4 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..867
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304449-rid9560453.denovo.196"
                     /strain="VREN0534"
                     /db_xref="taxon:1352"
     gene            complement(<1..210)
                     /locus_tag="IXT20_002863"
     CDS             complement(<1..210)
                     /locus_tag="IXT20_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HAQ4861236.1"
                     /translation="MDTITPTNFKENIGKVLQEVVHSNSEIEITRDSKNGLNDGVIMI
                     SKRKWELLQEELYLQRTGTWAYVSDL"
     gene            complement(309..623)
                     /locus_tag="IXT20_002864"
     CDS             complement(309..623)
                     /locus_tag="IXT20_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4861237.1"
                     /translation="MAEENKVVPFETLVINKEEFEQLFLDFFIQYDMIYDFSSSLDLF
                     ELEAEIIENSREKNGKIIISVEKFNELFSQTFEIDPDYYQPQEVFREFRNKIIGFSQT
                     TK"
BASE COUNT          285 a          172 c           90 g          320 t
ORIGIN      
        1 taaatcagat acataagccc atgtacccgt tcgttgtaaa taaagttctt cttgtaacaa
       61 ctcccatttt ctcttagaaa tcataatgac gccatcgttc aatccatttt tagaatccct
      121 agtaatttct atttcagaat ttgaatgaac tacttcttgc aacactttac ctatattttc
      181 tttgaagttt gttggagtaa tagtatccaa aatgaatacc tctcttctct atacctctat
      241 tatgaagtgc ccataaaata aagcaagcgt tgaggaataa ttctatcaac acttgcttta
      301 ttaagctttc attttgtagt ttgactaaaa ccaataatct tatttctaaa ctctcgaaaa
      361 acttcttgtg gttgataata gtctggatct atttcaaatg tttgtgagaa taattcattg
      421 aatttttcta ctgaaataat gattttacca ttcttctctc gactgttttc tattatttct
      481 gcttctaatt caaataaatc tagtgacgaa ctaaaatcat aaatcatgtc atattgaata
      541 aaaaaatcaa gaaaaagttg ctcaaactct tccttattta tcactaatgt ttcaaacgga
      601 acaactttat tttcctcagc cattctccca gttccatttt caaaaatcac aaaactgata
      661 ttaccttaaa aactactaac aaaataaaac ttataaagta aatggcaacc caactatgta
      721 accattctct ctacgtacta cgatttggta gtcctgcctt tcaatcggta attcataata
      781 gtaatttact gtttcttctt tgccactttc aatatactct ggattatcca atatcatatc
      841 atactcataa agttcttcac catcctt
//
LOCUS       DACGLE010000184          863 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0534
            SAMEA3304449-rid9560453.denovo.197, whole genome shotgun sequence.
ACCESSION   DACGLE010000184 DACGLE010000000
VERSION     DACGLE010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304449
            Sequence Read Archive: ERR985064
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 863)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 863)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702225.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702225.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 20:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,045
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 27 of 67
            Pseudo Genes (incomplete)         :: 25 of 67
            Pseudo Genes (internal stop)      :: 19 of 67
            Pseudo Genes (multiple problems)  :: 4 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..863
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304449-rid9560453.denovo.197"
                     /strain="VREN0534"
                     /db_xref="taxon:1352"
     gene            <1..861
                     /locus_tag="IXT20_002865"
     CDS             <1..861
                     /locus_tag="IXT20_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEnfa3 family transposase"
                     /protein_id="HAQ4861238.1"
                     /translation="EQEEIKVKTILQLKKEFKLNLLLSIAQLAKSTYYYWVKKLDKPD
                     KYSKIKQEITAIVKESRNSYGYRRVTLALKMKGYTINHKTVRKLMSQMGLTCQIRIKR
                     YKSYKGTVGKIAKNVLKRNFSVDTPNKKWVTDVTEFKIKGRKIYLSPILDLFNGEIIS
                     YSISTSPTYKLIEEMLQQAIKKKGTEGSLILHSDQGWQYQMPQYQKKLKENNIIQSMS
                     RKGNCLDNSVIENFFGVLKSEFFYREKFRSIEIFQSKLNEYIRWYNNKRIKLKLNGLS
                     PVEYRKQSIK"
BASE COUNT          374 a          112 c          140 g          237 t
ORIGIN      
        1 gagcaagaag aaatcaaggt taaaacaatc cttcagctca aaaaagaatt taaattgaat
       61 ctattacttt caattgcaca attagcgaag tcaacttatt attactgggt aaaaaaatta
      121 gataagccag ataaatatag caagataaag caagaaatta cagcgattgt aaaagaatca
      181 agaaactctt atggttatcg tagagtcact ttagcgttaa agatgaaagg atatacaatc
      241 aatcataaaa cagttagaaa attaatgtcc caaatgggac ttacctgtca aatccgaata
      301 aagcgatata aatcttataa aggaacagta ggaaaaattg ccaagaatgt gttaaaacga
      361 aatttttcag tagatacacc caataaaaaa tgggtgacag acgtcactga attcaagata
      421 aaaggaagaa aaatctatct atctcctata cttgatttat ttaacggaga aataattagt
      481 tatagtatat caactagtcc aacatataaa ctgattgaag aaatgcttca acaagcaatt
      541 aaaaaaaagg gaactgaggg ttcactgatt ctacattcag atcaaggatg gcagtatcag
      601 atgccacaat atcaaaaaaa attaaaagag aataacatta ttcaaagcat gtctagaaaa
      661 ggaaattgct tagataattc tgtaatagaa aatttttttg gtgtgcttaa atcagagttt
      721 ttttatcgag aaaaattccg atcaattgag atatttcaaa gtaaattaaa tgagtatatt
      781 aggtggtata ataacaaaag gataaaacta aagttaaatg gactatctcc agtagaatat
      841 cggaaacagt ccattaaata gtt
//
LOCUS       DACGLE010000185          841 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0534
            SAMEA3304449-rid9560453.denovo.198, whole genome shotgun sequence.
ACCESSION   DACGLE010000185 DACGLE010000000
VERSION     DACGLE010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304449
            Sequence Read Archive: ERR985064
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 841)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 841)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702225.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702225.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 20:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,045
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 27 of 67
            Pseudo Genes (incomplete)         :: 25 of 67
            Pseudo Genes (internal stop)      :: 19 of 67
            Pseudo Genes (multiple problems)  :: 4 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..841
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304449-rid9560453.denovo.198"
                     /strain="VREN0534"
                     /db_xref="taxon:1352"
     gene            complement(32..232)
                     /locus_tag="IXT20_002866"
     CDS             complement(32..232)
                     /locus_tag="IXT20_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAQ4861239.1"
                     /translation="MNNGTVKWFNADKGFGFITGEDGNDVFAHFSAIQGDGFKTLEEG
                     QAVSYDVEDGQRGPQATNIVKA"
BASE COUNT          272 a          143 c          134 g          292 t
ORIGIN      
        1 tttcgaaaag acggttaaaa gtcaattttt attaagcttt aacaatgttt gttgcttgag
       61 ggccacgttg accatcttca acatcgtaag acactgcttg gccttcttct aaagttttga
      121 agccgtcacc ttggatagct gaaaaatgtg caaatacatc atttccatct tcaccagtga
      181 taaagccaaa acctttgtct gcgttgaacc attttactgt accgttattc ataaataaat
      241 tcctcctgat acgtatcaat gtacgttttt tcgttgcaat aaatatttga taggcaaaag
      301 gagttttatc tatgtagata tatcgctcaa attacgtttc aaaatagatt actcatttaa
      361 tatatcatgc tttttctaat aacacaagga taatgcaatg aaatataaaa ttcattgtct
      421 tctattgtct tcgaatcgaa gaaaaagttg aataaccgcg attgattcac tttttgtttt
      481 cttgtttatt tgcttttatc tgcaactttt gactttaaaa tttcttaact atgaaaacct
      541 ttcaattggt ggtatactta taaatggaat ttcgttatca acgcgttcgt tgcctttcag
      601 caagtattta aagaaaggag aatggctatg acattctata acaaagatat acaatatcga
      661 gtaaccttgg atacagattt gaatctattt gtcgtttttg acaaagaaaa taccaatcat
      721 gtagcgacag gtgttacgat cgaacaagca gtgcaagaat taaaaaaatc tgcataagga
      781 gtggaaaccc tcatatggac gattctcgtt cttctcttta aacgcatcta ctggtagata
      841 a
//
LOCUS       DACGLE010000186          820 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0534
            SAMEA3304449-rid9560453.denovo.199, whole genome shotgun sequence.
ACCESSION   DACGLE010000186 DACGLE010000000
VERSION     DACGLE010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304449
            Sequence Read Archive: ERR985064
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 820)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 820)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702225.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702225.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 20:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,045
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 27 of 67
            Pseudo Genes (incomplete)         :: 25 of 67
            Pseudo Genes (internal stop)      :: 19 of 67
            Pseudo Genes (multiple problems)  :: 4 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..820
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304449-rid9560453.denovo.199"
                     /strain="VREN0534"
                     /db_xref="taxon:1352"
     gene            1..797
                     /locus_tag="IXT20_002867"
                     /pseudo
     CDS             1..797
                     /locus_tag="IXT20_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324322.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS256-like element ISEfm2 family transposase"
BASE COUNT          287 a          153 c          149 g          231 t
ORIGIN      
        1 actacacgcc ccaaaccatg tccaacatga ctaaagtgct gactgaagaa gtaaatgcat
       61 ttaaagttag aactctaaat gataagtatg tcgctatttt catggatgct acttatattc
      121 ctttaaaacg gcaaaccgtc tccaaagaag caatctatat tgccattggt atacgagaag
      181 acggcactaa agaagtacta agttatgcga ttgctccgac tgaatcgacc tacgtttgga
      241 atgagttgtt acaggacatt tactccagag gggttcatga tgtcttactg tttattaccg
      301 atggcttaaa aggcatgaaa gatacgattc accagattta tcctaaagct aaataccaac
      361 attgctgtgt tcacgtttcc cgtaatattg ctcataaagt acgtgtcaaa gatcgaaaag
      421 aaatctgtga tgattttaag gctgtttatc aagctagctc aaaggaagag gcaaatacct
      481 ttttagggag tatgattgag aagtagcaaa aaacttatcc taaagtgacg cagtcactga
      541 taaaaaatca agatttattg actttttatg agtttccgcc tggtatccgc cgaagcattt
      601 actcaactaa tttaatcgaa tctttcaaga aacaaatcaa gaaatacagc cgcagaaaag
      661 agcagtttca aaatgaagaa tcactagagc gtttcctagt atccatcttt gatacataca
      721 atcaaaaatt cttaaataga agccataaag gcttccaaca agtgacggat acattagctt
      781 caatgtttac tgagtaaccc attattttgc agaaggacga
//
LOCUS       DACGLE010000187          789 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0534
            SAMEA3304449-rid9560453.denovo.200, whole genome shotgun sequence.
ACCESSION   DACGLE010000187 DACGLE010000000
VERSION     DACGLE010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304449
            Sequence Read Archive: ERR985064
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 789)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 789)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702225.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702225.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 20:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,045
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 27 of 67
            Pseudo Genes (incomplete)         :: 25 of 67
            Pseudo Genes (internal stop)      :: 19 of 67
            Pseudo Genes (multiple problems)  :: 4 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..789
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304449-rid9560453.denovo.200"
                     /strain="VREN0534"
                     /db_xref="taxon:1352"
     gene            <1..780
                     /locus_tag="IXT20_002868"
     CDS             <1..780
                     /locus_tag="IXT20_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAQ4861240.1"
                     /translation="DMLDYYYFEKNLSYNYVIHLKTFTGMIFKFANRRYGLKYNPVNG
                     VSLARKPKTNEEIKKEKDGYLSKEEVQMIASKQKSSHQQSRYSLITKFLFLTGLRYGE
                     LISLTESDYDGNKITVSGTYDYELKIKTTTKNTGSYRTIELSSNAKEIIDQLIEENKL
                     IKNNKDKYIFISKNGNPISIQAYNQSLRAVSKELNIDKKVSSHMLRHSHISLLTELGI
                     PLKAIMDRVGHEDSKTTLKIYTHTTKNMQNQLVEKLGKIEI"
BASE COUNT          338 a          107 c          102 g          242 t
ORIGIN      
        1 gatatgctag attactacta ttttgaaaaa aatttatcat ataattatgt aatacattta
       61 aaaactttta caggaatgat ttttaaattc gccaaccgtc gatatggatt aaaatataat
      121 cccgtaaatg gggtttcttt agcaagaaaa cctaagacaa acgaagaaat aaaaaaagaa
      181 aaagatggtt atttatcaaa agaagaagtt caaatgattg ctagtaaaca aaaaagttca
      241 catcaacagt cacgttattc tttaattact aaatttttgt ttttaactgg actacgttat
      301 ggtgaattga tatctttgac tgaatctgat tatgatggta ataaaattac ggtttctggt
      361 acttatgatt acgagttaaa aattaaaaca acaactaaaa atacaggttc ttatagaacc
      421 attgaattat caagtaatgc taaagaaatc atagatcaat taattgaaga aaacaaatta
      481 attaagaata ataaagataa atacattttc atctctaaaa atggtaaccc gatatctatt
      541 caagcatata atcaatcttt aagagcagta tccaaagaat taaacataga taaaaaagtt
      601 tcttctcaca tgttaaggca ttcccacatt tctctactta cagaattagg aatcccatta
      661 aaagccatta tggatcgtgt aggacatgaa gattcaaaaa caacattaaa gatttatact
      721 catacaacta aaaatatgca aaatcaacta gtagaaaaac taggaaaaat agaaatttaa
      781 attgcccct
//
LOCUS       DACGLE010000188          779 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0534
            SAMEA3304449-rid9560453.denovo.201, whole genome shotgun sequence.
ACCESSION   DACGLE010000188 DACGLE010000000
VERSION     DACGLE010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304449
            Sequence Read Archive: ERR985064
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 779)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 779)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702225.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702225.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 20:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,045
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 27 of 67
            Pseudo Genes (incomplete)         :: 25 of 67
            Pseudo Genes (internal stop)      :: 19 of 67
            Pseudo Genes (multiple problems)  :: 4 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..779
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304449-rid9560453.denovo.201"
                     /strain="VREN0534"
                     /db_xref="taxon:1352"
     gene            118..>779
                     /locus_tag="IXT20_002869"
     CDS             118..>779
                     /locus_tag="IXT20_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HAQ4861241.1"
                     /translation="MRSSKYTEHYTDTFITFKEIMRTVSNIYHNCVPDKIKNRRNTDK
                     LKQRDTVIIACVIWGIINGYTSQRATYRAVCSVLFPNGDFPSRSRFTRLSSNLAYTIK
                     IIRYFFIKKLTKGELVGIIDSFPSPLCKPVRNRQAKLLNQIAKVGYNSTKKSYFYGLK
                     IHMIVTKTGFPITYSITNPGVHDVKVLETLSEEANLPNILGDKGYISHKIHEKLALKG
                     IT"
BASE COUNT          280 a          139 c          131 g          229 t
ORIGIN      
        1 ataaaaaccc gtggtgctag ttaatttttt cctagaggaa aatgtctaaa catgttggta
       61 aatcaacaaa aaaacgcata tagtagtctc aaaactccca agaaatgagg actacatatg
      121 cgttcatcaa aatatactga acattataca gatactttta taacattcaa ggaaattatg
      181 agaactgttt ctaacatata ccacaattgt gtaccagata agattaaaaa ccgaagaaat
      241 actgataaac tgaagcaacg cgatacagtg attattgctt gtgttatatg gggcataatt
      301 aatggctata ctagccaaag agccacgtat agagcggttt gttccgtctt atttcctaat
      361 ggtgactttc ctagtaggag tcgattcact cgcttaagtt caaacttagc ttacactatc
      421 aagattattc gatacttttt catcaagaag ctcaccaaag gtgaactagt cggaattata
      481 gatagttttc ctagtccatt atgtaaacca gttagaaata gacaagcaaa actattgaat
      541 caaatagcca aagttggcta taactcaaca aaaaaatctt atttttatgg tcttaaaatt
      601 catatgatcg ttactaagac tggctttccg attacttact caatcacgaa tccaggtgtc
      661 catgacgtga aagtgttaga gactttatcg gaagaagcga atcttcccaa tatcctaggg
      721 gacaaaggat atattagcca taaaatccat gaaaagcttg cccttaaggg cattactat
//
LOCUS       DACGLE010000189          775 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0534
            SAMEA3304449-rid9560453.denovo.202, whole genome shotgun sequence.
ACCESSION   DACGLE010000189 DACGLE010000000
VERSION     DACGLE010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304449
            Sequence Read Archive: ERR985064
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 775)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 775)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702225.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702225.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 20:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,045
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 27 of 67
            Pseudo Genes (incomplete)         :: 25 of 67
            Pseudo Genes (internal stop)      :: 19 of 67
            Pseudo Genes (multiple problems)  :: 4 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..775
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304449-rid9560453.denovo.202"
                     /strain="VREN0534"
                     /db_xref="taxon:1352"
     gene            28..>775
                     /locus_tag="IXT20_002870"
     CDS             28..>775
                     /locus_tag="IXT20_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAQ4861242.1"
                     /translation="MTYTHLTTDELVIIESYFKMNQSVAKTAHCLNRSRQTIHKVYLF
                     FKQGKSALEYYQQYKKNKSNCGRRPLVLPEEQSEYIQRKVVQGWTPDVIVGRAAFPIS
                     CSARTIYRMFKKGLFDSSDLPMKGKRKPNGHQERRGKQTFRRSIHEREKDYSQFSNEF
                     GHLEGDTIVGLKHKSAVITLVERLSKVIITLKPCGRQAIDIEKKLNQWFESVPKNLFK
                     SITFDCGKEFSNWKQISNVNDIAIYFADPGT"
BASE COUNT          270 a          137 c          146 g          222 t
ORIGIN      
        1 ccaaagaatt ataaggagtg agtacaaatg acctataccc atcttacaac ggatgaactt
       61 gtaataatag agtcttattt caaaatgaat caatctgttg ctaaaactgc ccattgcttg
      121 aatcgttcaa gacaaacgat ccataaagta tatctgttct tcaagcaagg aaaatcagcc
      181 ttagaatatt atcaacagta taagaaaaac aaatcaaact gtggtagacg tccgcttgtt
      241 ttacccgagg aacaatcaga atatattcaa agaaaggttg ttcaaggatg gacacccgat
      301 gtgattgttg gtcgtgcagc gtttcctatt agttgttctg ctcgtaccat ttatcgtatg
      361 ttcaaaaagg ggctatttga ttcttctgac ttaccgatga aaggcaagcg taaaccgaat
      421 ggacatcaag aaagacgtgg aaaacaaact ttccgccgct ctattcatga acgtgaaaag
      481 gattatagcc aattctcaaa tgagtttggt caccttgaag gtgacactat cgtaggtctg
      541 aaacataaaa gtgctgtaat taccttagtt gaacgattat caaaagttat catcacattg
      601 aaaccgtgtg gtagacaagc gattgatatt gaaaaaaaat taaatcaatg gtttgaatct
      661 gtaccgaaaa acctattcaa atccatcact tttgattgtg gaaaggaatt ttcaaattgg
      721 aaacagatca gtaatgtcaa tgatattgcc atttatttcg ctgatccagg aacgc
//
LOCUS       DACGLE010000190          774 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0534
            SAMEA3304449-rid9560453.denovo.203, whole genome shotgun sequence.
ACCESSION   DACGLE010000190 DACGLE010000000
VERSION     DACGLE010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304449
            Sequence Read Archive: ERR985064
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 774)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 774)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702225.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702225.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 20:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,045
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 27 of 67
            Pseudo Genes (incomplete)         :: 25 of 67
            Pseudo Genes (internal stop)      :: 19 of 67
            Pseudo Genes (multiple problems)  :: 4 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..774
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304449-rid9560453.denovo.203"
                     /strain="VREN0534"
                     /db_xref="taxon:1352"
     gene            <1..693
                     /gene="tnpB"
                     /locus_tag="IXT20_002871"
     CDS             <1..693
                     /gene="tnpB"
                     /locus_tag="IXT20_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family element transposase accessory
                     protein TnpB"
                     /protein_id="HAQ4861243.1"
                     /translation="ATISRHSSGKYYISLLCKEEIIELPKTNSTIGIDLGIMDFAILS
                     DGQKIDNNKFTSKMEKKLKREQRKLSRRALLAKNKGTNLFEAKNYQKQKRKVARLHEK
                     VMNQRTDFLNKLSTEMIKNHDIICIEDLNTKGMLRNHKLAKSISDVSWSKFVTKLQYK
                     ADWYGRKIIKVDKWFPSSQICSECGHKDGKKSLEIREWTCPVCHTHHDRDINASINIL
                     TEGLRLHSMGLA"
BASE COUNT          287 a          116 c          144 g          227 t
ORIGIN      
        1 gctacaatat cacgtcattc tagcggtaaa tactatattt ctttgttgtg taaagaagaa
       61 attatcgaat tacctaaaac taattccaca attggtattg acttaggtat tatggacttt
      121 gcgattcttt ctgatggaca aaaaattgat aataataaat tcacgtccaa aatggaaaag
      181 aaactaaagc gtgaacaacg caaattgtct aggcgtgctt tattggctaa aaataaaggt
      241 accaatctat ttgaagctaa aaattatcaa aaacaaaaac gcaaagtagc tagattgcat
      301 gaaaaagtaa tgaatcaacg tactgatttt ctgaataagt tgagtacaga aatgatcaaa
      361 aatcacgata ttatctgtat tgaagactta aacacaaaag gtatgttgcg aaatcataaa
      421 ttagctaaaa gtatttctga tgtttcttgg tcaaagtttg tgactaaatt acaatacaag
      481 gctgactggt atggacgaaa aatcatcaaa gtagataaat ggtttccgtc tagtcagatt
      541 tgttcagagt gcggacataa agatggcaag aaatctcttg aaattcgaga atggacttgt
      601 cctgtttgtc atactcatca tgatcgtgat atcaatgcta gtatcaatat tctgaccgaa
      661 ggtctaagac tacattctat gggattggct tagatccaat catcaagaac cgtaggaact
      721 acggggatag cttggtaaat aagagacacc gctgttagta aagaaatacg ctat
//
LOCUS       DACGLE010000191          737 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0534
            SAMEA3304449-rid9560453.denovo.204, whole genome shotgun sequence.
ACCESSION   DACGLE010000191 DACGLE010000000
VERSION     DACGLE010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304449
            Sequence Read Archive: ERR985064
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 737)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 737)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702225.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702225.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 20:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,045
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 27 of 67
            Pseudo Genes (incomplete)         :: 25 of 67
            Pseudo Genes (internal stop)      :: 19 of 67
            Pseudo Genes (multiple problems)  :: 4 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..737
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304449-rid9560453.denovo.204"
                     /strain="VREN0534"
                     /db_xref="taxon:1352"
     gene            <1..699
                     /locus_tag="IXT20_002872"
     CDS             <1..699
                     /locus_tag="IXT20_002872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAQ4861244.1"
                     /translation="RLPYLERYFSRFSLATREAVQLIVIDMYAPYVSLVKKLFPNAQL
                     IIDRFHIVQHIGRTFLNHRVKETTVRLKGNSHSQRGLGKKLKRYWKLLQKEEAKLNYE
                     KRVWRASFKDYLTESEIADRLLAACPNLRQGYQLYQDILYAVKKRDQSLFEDCLTRDI
                     SGLPETYTTTLRTFKKFLPQIQNALHYSYSNGPLECLNNHIKVLKRNAYGFRNFYNFK
                     LRIMIRHGKTFLTK"
BASE COUNT          234 a          150 c          137 g          216 t
ORIGIN      
        1 cgcttgcctt atttagaacg ctatttttct cggttttcat tagcgactcg tgaagccgtt
       61 caattgattg ttattgatat gtatgcgcct tatgtttcgt tggtgaaaaa gcttttcccg
      121 aatgctcagt tgatcatcga tcgtttccac atcgtccaac atattggcag aactttcttg
      181 aatcatcgag tgaaagaaac cacggtacga cttaaaggaa attcccactc tcaacgaggg
      241 ttagggaaaa aattgaagcg ctattggaaa ctcctacaaa aagaagaagc aaagttaaac
      301 tatgaaaaac gggtttggcg tgccagtttt aaagattatt tgactgaaag tgagattgcc
      361 gatcgactgt tagctgcttg tcccaactta cgccaaggct atcagttata ccaagacatt
      421 ctttatgcgg taaagaaaag agatcagtca ctatttgagg attgtttaac tagagacatt
      481 agcggtctgc cagaaaccta tacaaccact ttacgaacgt ttaaaaagtt tctgccacag
      541 atccaaaatg cgcttcacta cagctattca aacggaccat tggaatgctt aaacaaccac
      601 atcaaagtat taaaacgcaa tgcgtacggc tttcgcaact tttacaattt caaactacga
      661 attatgattc gtcatggcaa aacattttta accaaataaa cagaacagaa agaggttttc
      721 ttctttctgt tctgtcc
//
LOCUS       DACGLE010000192          721 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0534
            SAMEA3304449-rid9560453.denovo.205, whole genome shotgun sequence.
ACCESSION   DACGLE010000192 DACGLE010000000
VERSION     DACGLE010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304449
            Sequence Read Archive: ERR985064
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 721)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 721)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702225.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702225.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 20:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,045
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 27 of 67
            Pseudo Genes (incomplete)         :: 25 of 67
            Pseudo Genes (internal stop)      :: 19 of 67
            Pseudo Genes (multiple problems)  :: 4 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..721
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304449-rid9560453.denovo.205"
                     /strain="VREN0534"
                     /db_xref="taxon:1352"
     gene            30..721
                     /locus_tag="IXT20_002873"
                     /pseudo
     CDS             30..721
                     /locus_tag="IXT20_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          294 a          123 c          135 g          169 t
ORIGIN      
        1 tagaagaaaa cataccagga ggaattttta tgtctaagag aacacgaaga actttttcac
       61 aagaattcaa gcaacaaatc gtcaatcttt acttagctgg aaagccacgt gtagaaatca
      121 ttcgagaata tgaactaacg gcttcagcat ttgacaaatg ggtaaagcaa tctaaaacga
      181 gtggttcatt caaagaaaaa gataatctta cgcctgaaca aaaagaattg ttagaactac
      241 gtaaaagaaa ccagcaatta gaaatggaaa atgatatttt aaagcaagca gcgctgatat
      301 tcggacgaag agacaagtaa tcgatgcgaa taagcatctt taccctatat cagcgatgtg
      361 cagaatatta ggtctatcac gtcagtccta ttattatcaa tcaaaaccaa agaaagacga
      421 atcagaactt gaagaagtag tcgctgaaga atttatccgc agccgaaagg cctacggctc
      481 aagaaaaata aaaaaagcct tatcaaaacg aggcattcag atcagccgac gaaaaattag
      541 tagaatcatg aaaaatagag gattaaaatc gagctatact gttgcttatt ttaaagtaca
      601 tcattctact tgcaatgaag ccaaaacgac aaacgtattg aatcgtaaat tcttaagaga
      661 caacccatta gaagcgatcg taacagactt gacttatgta cgagtcggga aaaaatggaa
      721 t
//
LOCUS       DACGLE010000193          679 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0534
            SAMEA3304449-rid9560453.denovo.206, whole genome shotgun sequence.
ACCESSION   DACGLE010000193 DACGLE010000000
VERSION     DACGLE010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304449
            Sequence Read Archive: ERR985064
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 679)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 679)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702225.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702225.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 20:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,045
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 27 of 67
            Pseudo Genes (incomplete)         :: 25 of 67
            Pseudo Genes (internal stop)      :: 19 of 67
            Pseudo Genes (multiple problems)  :: 4 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..679
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304449-rid9560453.denovo.206"
                     /strain="VREN0534"
                     /db_xref="taxon:1352"
BASE COUNT          240 a          114 c           80 g          245 t
ORIGIN      
        1 ccaaattact tcatcaaaac attgatataa tttttattat atcagtattt attcttgata
       61 cataaatgag aaaataaata ctttaaatct attgactaca agtagtcatt aagtcttcat
      121 aagatgtaca atatgacatt aaaaaaataa aagcttacga ccatccgtta aatctgacac
      181 taaataatgg actgctttct tttgtattag caggttacgt tgactatgaa tattcaccag
      241 attatttgga gaaacctctt caacacgaga acaatgatat taattttaga tttgtctacg
      301 agtcaaaaag taaaacatgg ttagttgatc aattaagcac gataaacaca agcttccatg
      361 aattacctaa acctgaaaat gaagttgaga tggtcattaa aatcttctaa aaaagaagcg
      421 ttgaagggga aatattcctt tcaacgctct tttttatatg cttagaaaca taaataaatt
      481 gtatactttc tttttagccc ctattacttt tcacgaaaaa tatctttttt tcgacgctag
      541 cgtttctcta cttctaatac tgtacacagt ttttgtatta ttgaaaaatt actcattatt
      601 tagcattaat ccacatcgtt ttatcaatcg accttcatac taaactactt taaagtcatc
      661 ttccttctcg tttttcatt
//
LOCUS       DACGLE010000194          584 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0534
            SAMEA3304449-rid9560453.denovo.207, whole genome shotgun sequence.
ACCESSION   DACGLE010000194 DACGLE010000000
VERSION     DACGLE010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304449
            Sequence Read Archive: ERR985064
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 584)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 584)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702225.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702225.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 20:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,045
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 27 of 67
            Pseudo Genes (incomplete)         :: 25 of 67
            Pseudo Genes (internal stop)      :: 19 of 67
            Pseudo Genes (multiple problems)  :: 4 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..584
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304449-rid9560453.denovo.207"
                     /strain="VREN0534"
                     /db_xref="taxon:1352"
     gene            complement(<1..217)
                     /locus_tag="IXT20_002874"
     CDS             complement(<1..217)
                     /locus_tag="IXT20_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4861245.1"
                     /translation="MEDKKEKLFDLIANAYNSKSYNNVEVKEGFLQDAKKIREDKPYP
                     EIIISVYKHLSKLKAEERMDRLQLTRQK"
     gene            complement(207..389)
                     /locus_tag="IXT20_002875"
     CDS             complement(207..389)
                     /locus_tag="IXT20_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterocin"
                     /protein_id="HAQ4861246.1"
                     /translation="MSVSTLGITVDAATYYGNGVYCNTQKCWVDWSRARSEIVDRGVK
                     AYVNGFTKVLGGVGGR"
BASE COUNT          191 a          114 c           53 g          226 t
ORIGIN      
        1 ttttctgtct agttaattgt agcctatcca tgcgttcctc agcttttaat ttagataaat
       61 gtttgtacac tgatataata atttcaggat atggtttatc ctctcgtatt ttcttagcat
      121 cttgaagaaa tccttctttt acttctacat tattatatga ctttgaatta tatgcattcg
      181 caattaagtc aaaaagtttt tcttttttat cttccaccga ctccacctag cacttttgta
      241 aaaccattaa cgtaagcttt tactcctcta tcaacaatct cagagcgagc tctactccaa
      301 tctacccaac atttttgagt attacaataa acaccatttc cataataagt tgcagcatct
      361 actgtaattc ctaaagtaga tacactcaat aaaacaattc ctaaaatcaa aaaatactaa
      421 caaatttttt cttcatataa ttgcctccct tttttataaa acaattatat gtaattttca
      481 gaacattttt gcaaaatttt cataaatagt atctttagtg tcataatcgt caccaatatt
      541 ctgtaagatc aatcaaattt tatctcacta ataataatag tctt
//
LOCUS       DACGLE010000195          582 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0534
            SAMEA3304449-rid9560453.denovo.208, whole genome shotgun sequence.
ACCESSION   DACGLE010000195 DACGLE010000000
VERSION     DACGLE010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304449
            Sequence Read Archive: ERR985064
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 582)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 582)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702225.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702225.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 20:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,045
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 27 of 67
            Pseudo Genes (incomplete)         :: 25 of 67
            Pseudo Genes (internal stop)      :: 19 of 67
            Pseudo Genes (multiple problems)  :: 4 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..582
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304449-rid9560453.denovo.208"
                     /strain="VREN0534"
                     /db_xref="taxon:1352"
     gene            144..>582
                     /locus_tag="IXT20_002876"
     CDS             144..>582
                     /locus_tag="IXT20_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HAQ4861247.1"
                     /translation="MGATITSKGRKKNLSSLLQYAANPSKKEKCVAVSGINCSDDSEE
                     ALQEMNLVRTKMNKSKDIAQAFHVIHSFDKSTSKELSIDKMHEIAVEFAEKAFPNSQI
                     IVASHNDKDHFHSHLVINNINMETCKRLRIDPKDLELMRKINDQ"
BASE COUNT          233 a           80 c          108 g          161 t
ORIGIN      
        1 gaagtagaaa aagaatattt cgaatattgg atagaaaagc aaactagacc gggtacgaaa
       61 gaagagtatt cccaaaaagt tgatcggaat ttaattcact caattcagat tattgcaagt
      121 ttaaaagatg aggtgaaaca actttgggag ctactataac atcgaaagga cgcaagaaaa
      181 atttatcctc gctattacaa tatgcagcta atccttcaaa aaaagaaaaa tgtgttgcag
      241 tctcaggaat aaattgtagt gatgatagtg aggaagcact gcaagaaatg aatttagtac
      301 gtaccaagat gaataaatct aaagatatag cacaagcgtt tcatgttatt cattcgtttg
      361 ataagagtac ttctaaagag ttaagtattg ataaaatgca cgaaatagct gtcgaatttg
      421 cagagaaagc ttttccaaat tcgcaaatca ttgttgcaag tcacaatgat aaagatcatt
      481 ttcatagtca tcttgtcatt aataatatta acatggaaac atgtaaaagg ttgcgtattg
      541 accctaaaga tttagaacta atgcgaaaaa ttaacgatca ag
//
LOCUS       DACGLE010000196          569 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0534
            SAMEA3304449-rid9560453.denovo.209, whole genome shotgun sequence.
ACCESSION   DACGLE010000196 DACGLE010000000
VERSION     DACGLE010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304449
            Sequence Read Archive: ERR985064
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 569)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 569)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702225.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702225.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 20:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,045
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 27 of 67
            Pseudo Genes (incomplete)         :: 25 of 67
            Pseudo Genes (internal stop)      :: 19 of 67
            Pseudo Genes (multiple problems)  :: 4 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..569
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304449-rid9560453.denovo.209"
                     /strain="VREN0534"
                     /db_xref="taxon:1352"
     gene            <1..>569
                     /locus_tag="IXT20_002877"
     CDS             <1..>569
                     /locus_tag="IXT20_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAQ4861248.1"
                     /translation="SLNQLLPEVVCFDEFKSVKNVSGAMSFVMMDGKTHQLIDIVENR
                     QLNPLRGYFLRYPRKVREQVRLVVSDFYTPYRTLVKERFPNARIVADRFHISQHIGRA
                     FTNHRIQVMKTFKKRDRRSKHLKKYWRLLQKNAWELKGQHRYWRPSFRDHLTEAEIVD
                     RLLSYDDSLKRGYEVYQDFLSAIRRQAVP"
BASE COUNT          162 a          127 c          128 g          152 t
ORIGIN      
        1 tttctttgaa ccagctgtta ccagaagtgg tgtgtttcga tgaattcaaa tcagtgaaaa
       61 atgtctcggg agccatgagc tttgtcatga tggacggcaa gacccatcaa ctgattgata
      121 ttgtggaaaa tcggcaactc aatcctttac gcggctattt cttgcgctac cctagaaaag
      181 tccgtgaaca agtacgtcta gtggtttcag atttctatac accctatcgc acattggtga
      241 aagagcgttt tccgaatgcc cgaatcgtcg ccgatcgctt tcatattagc caacacatcg
      301 gtcgggcctt taccaatcac cgaattcaag taatgaagac ctttaaaaaa agggatcgtc
      361 ggtcgaagca cttaaagaaa tactggagac tacttcaaaa gaatgcgtgg gaattgaagg
      421 gccagcatcg ctactggcgt ccgtcatttc gagatcatct gacagaagcg gagatagtcg
      481 atcggctatt atcttatgat gactccttaa agcgaggcta cgaagtctac caggattttc
      541 tttccgctat cagaagacaa gctgttcca
//
LOCUS       DACGLE010000197          548 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0534
            SAMEA3304449-rid9560453.denovo.210, whole genome shotgun sequence.
ACCESSION   DACGLE010000197 DACGLE010000000
VERSION     DACGLE010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304449
            Sequence Read Archive: ERR985064
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 548)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 548)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702225.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702225.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 20:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,045
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 27 of 67
            Pseudo Genes (incomplete)         :: 25 of 67
            Pseudo Genes (internal stop)      :: 19 of 67
            Pseudo Genes (multiple problems)  :: 4 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..548
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304449-rid9560453.denovo.210"
                     /strain="VREN0534"
                     /db_xref="taxon:1352"
     gene            complement(<1..>548)
                     /locus_tag="IXT20_002878"
     CDS             complement(<1..>548)
                     /locus_tag="IXT20_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019117172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HAQ4861249.1"
                     /translation="SLLYNSGAITELGSVDKGTTRTDNTLLERQRGITIQTGITSFQW
                     ENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAKDGVQAQTRILFHALRKMGIPT
                     IFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVELYPNMCVTNFTESEQWDTVIEG
                     NDDLLEKYMSGKSLEALELEQE"
BASE COUNT          147 a          119 c           84 g          198 t
ORIGIN      
        1 tcctcttgtt cgagttccaa tgcttctaat gatttaccgg acatatattt ctctaaaagg
       61 tcatcgtttc cctctattac cgtatcccat tgttcagatt cggtaaagtt cgtcacacac
      121 atattaggat acagttctac cttctgtttg attacaattt cggcagaaag tttctcttta
      181 atatcctgat aaaccgttga taaatcaatt ccattttggt caatcttatt gataaaaaag
      241 attgtgggaa tccccatttt cctaagtgca tgaaataata tacgagtttg tgcttgtacg
      301 ccatcttttg cagaaatcag tagaattgcc ccatctaaaa ctgataatga acgatatact
      361 tctgctaaga aatccatatg tcctggcgtg tctatgatgt tcaccttcgt attttcccac
      421 tgaaaagagg ttattcctgt ctgaattgta attcctctct gacgttctaa aagcgtatta
      481 tccgtcctcg ttgtaccttt gtccacgctt cctaattctg taatcgctcc actgttatat
      541 aataagct
//
LOCUS       DACGLE010000198          528 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0534
            SAMEA3304449-rid9560453.denovo.211, whole genome shotgun sequence.
ACCESSION   DACGLE010000198 DACGLE010000000
VERSION     DACGLE010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304449
            Sequence Read Archive: ERR985064
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 528)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 528)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702225.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702225.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 20:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,045
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 27 of 67
            Pseudo Genes (incomplete)         :: 25 of 67
            Pseudo Genes (internal stop)      :: 19 of 67
            Pseudo Genes (multiple problems)  :: 4 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..528
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304449-rid9560453.denovo.211"
                     /strain="VREN0534"
                     /db_xref="taxon:1352"
     gene            complement(5..90)
                     /locus_tag="IXT20_002879"
     tRNA            complement(5..90)
                     /locus_tag="IXT20_002879"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(54..56),aa:Leu,seq:taa)
     gene            complement(107..178)
                     /locus_tag="IXT20_002880"
     tRNA            complement(107..178)
                     /locus_tag="IXT20_002880"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(144..146),aa:Gly,seq:gcc)
     gene            complement(190..262)
                     /locus_tag="IXT20_002881"
     tRNA            complement(190..262)
                     /locus_tag="IXT20_002881"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(227..229),aa:Thr,seq:tgt)
     gene            complement(280..361)
                     /locus_tag="IXT20_002882"
     tRNA            complement(280..361)
                     /locus_tag="IXT20_002882"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(325..327),aa:Leu,seq:tag)
     gene            complement(369..441)
                     /locus_tag="IXT20_002883"
     tRNA            complement(369..441)
                     /locus_tag="IXT20_002883"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(406..408),aa:Lys,seq:ttt)
     gene            complement(445..517)
                     /locus_tag="IXT20_002884"
     tRNA            complement(445..517)
                     /locus_tag="IXT20_002884"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(482..484),aa:Val,seq:tac)
BASE COUNT          135 a          152 c          128 g          113 t
ORIGIN      
        1 gaaatgccga ggaccggaat cgaaccggta cggtgatcac tcaccgcagg attttaagtc
       61 ctgtgcgtct gccagttccg ccaccccggc gcgattgctt tggcaaagcg gaaaacgggg
      121 ttcgaacccg cgacccccac cttggcaagg tggtgctcta ccactgagct atttccgcgt
      181 gtttaaagat gccggctaaa ggacttgaac cctcgaccct ctgattacaa atcagatgct
      241 ctaccaactg agctaagccg gcatgtaaaa cttaaattat gcgggtgaag ggacttgaac
      301 ccccacgccg taaggcgcta gatcctaaat ctagtgcgtc tgccaattcc gccacacccg
      361 caaaaatatg agccgtacag ggctcgaacc tgtgaccctc tgattaaaag tcagatgctc
      421 taccaactga gctaacggct catatggagg ttaacgggat cgaaccgctg accctctgct
      481 tgtaaggcag atgctctccc agctgagcta aacctccatg gataactt
//
LOCUS       DACGLE010000199          517 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0534
            SAMEA3304449-rid9560453.denovo.212, whole genome shotgun sequence.
ACCESSION   DACGLE010000199 DACGLE010000000
VERSION     DACGLE010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304449
            Sequence Read Archive: ERR985064
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 517)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 517)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702225.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702225.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 20:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,045
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 27 of 67
            Pseudo Genes (incomplete)         :: 25 of 67
            Pseudo Genes (internal stop)      :: 19 of 67
            Pseudo Genes (multiple problems)  :: 4 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..517
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304449-rid9560453.denovo.212"
                     /strain="VREN0534"
                     /db_xref="taxon:1352"
BASE COUNT          169 a           87 c          117 g          144 t
ORIGIN      
        1 tatccataaa actaatataa agcttggtac gattctgttt gtaagacata cgaatagtat
       61 cagaggtagg agccaagctg cctgatatta aattatcgat aaagtagaaa ggttaagcat
      121 tagccgctgc aataactaat gctaggagac agcaacgtgt aaccattacc gcacgtgttt
      181 ctgaatggtg gtagcgaccg atttgaacgg ctagggcgga aatacggtgc aaatgcacgg
      241 tggtttcatg caatgcttac agggcgtatt ttgtaagggt atgtaacgga gataattccg
      301 acggtaatgg atgtgagagg ccaagccgac gctgacggta gggcaaacaa acatagttgc
      361 actctagcga tcgttattta tttttaacgg tcaaactagg gtacaactat gcttaaaaca
      421 cttcgcctca acttctggta gcaaaagtga ttttgaaagt caaaatattt tttatgagtt
      481 atttacaatt ctaaaaaaca agtgtattaa tctacta
//
LOCUS       DACGLE010000200          506 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0534
            SAMEA3304449-rid9560453.denovo.213, whole genome shotgun sequence.
ACCESSION   DACGLE010000200 DACGLE010000000
VERSION     DACGLE010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304449
            Sequence Read Archive: ERR985064
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 506)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 506)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702225.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702225.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 20:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,045
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 27 of 67
            Pseudo Genes (incomplete)         :: 25 of 67
            Pseudo Genes (internal stop)      :: 19 of 67
            Pseudo Genes (multiple problems)  :: 4 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..506
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304449-rid9560453.denovo.213"
                     /strain="VREN0534"
                     /db_xref="taxon:1352"
     gene            <1..>506
                     /locus_tag="IXT20_002885"
     CDS             <1..>506
                     /locus_tag="IXT20_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycoside hydrolase family 125 protein"
                     /protein_id="HAQ4861250.1"
                     /translation="EANNAGHQDDETQMNPWVWERKYEIDSLCYPIQLAYLLYQNTGR
                     TDQFSEDFIRGIEKVLTVFETEQNHQDSPYTFQRFDDRPEDTLVNHGRETTVAPTGMT
                     WSGFRPSDDACHYGYLVPSNMFAVVILDYLVEIFQDILKETTLIDRIIKLKKRSMMVS
                     KNLVRFLI"
BASE COUNT          153 a           89 c          117 g          147 t
ORIGIN      
        1 aagaggccaa caatgcgggg catcaggatg acgaaacaca aatgaatcct tgggtctggg
       61 aacgaaagta tgaaattgat tctctttgct atccaatcca gctggcttat ttgttatatc
      121 agaatactgg ccgtacagat caatttagtg aagactttat tagggggatc gagaaggtcc
      181 tgacggtttt tgaaacggaa cagaaccatc aagactcacc ctacactttt caacggtttg
      241 atgatcgacc ggaagatacg ttagtcaatc acggacggga aacgacagtt gcaccaacgg
      301 gtatgacttg gtcaggtttt cgtccaagtg atgatgcttg ccattatggt tatcttgttc
      361 cgtcaaatat gtttgcggtg gtcatccttg attatttagt agagattttc caagatattt
      421 tgaaggagac gacactgatt gaccgtatta tcaagttaaa aaagcgatca atgatggtat
      481 ccaaaaattt ggtaagattt ttaata
//
LOCUS       DACGLE010000201          487 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0534
            SAMEA3304449-rid9560453.denovo.214, whole genome shotgun sequence.
ACCESSION   DACGLE010000201 DACGLE010000000
VERSION     DACGLE010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304449
            Sequence Read Archive: ERR985064
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 487)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 487)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702225.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702225.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 20:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,045
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 27 of 67
            Pseudo Genes (incomplete)         :: 25 of 67
            Pseudo Genes (internal stop)      :: 19 of 67
            Pseudo Genes (multiple problems)  :: 4 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..487
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304449-rid9560453.denovo.214"
                     /strain="VREN0534"
                     /db_xref="taxon:1352"
     gene            complement(<1..>487)
                     /locus_tag="IXT20_002886"
     CDS             complement(<1..>487)
                     /locus_tag="IXT20_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HAQ4861251.1"
                     /translation="IHRRFYTSICHQKITTDTTEFKYHEVDAKGIIRQKKLYLDPFMD
                     MFNSEILSYRISEKPNALAVMEGLEEAIQTTNDCPYRRTFHSDQGWAYQMKAYRNKLK
                     EYKIFQSMSRKGNCLDNSLMENFFGLLKQEIFHGKVYNCFEELKSAIDSYIYYYNNER
                     IK"
BASE COUNT          142 a           92 c           86 g          167 t
ORIGIN      
        1 gttttatccg ttcattattg tagtaataga tatagctatc gatggctgat tttaattcct
       61 cgaaacagtt ataaactttc ccgtgaaaaa tctcttgttt taataagcca aaaaaattct
      121 ccataagtga attatctaag cagttccctt tccgagacat gctttgaaag attttgtact
      181 ccttcaattt gtttctataa gctttcattt gatacgccca accttgatct gagtgaaacg
      241 tacgacgata gggacaatcg ttagtggtct ggatagcttc ctccaaacct tccattaccg
      301 ctaatgcatt gggcttttca gagatacgat aggataaaat ctcactattg aacatatcca
      361 taaaaggatc tagatacaat ttcttttgac ggataatgcc tttggcatcc acttcatggt
      421 atttaaattc agttgtatcc gtcgtgattt tctgatggca gatactggtg tagaaacgac
      481 gatggat
//
LOCUS       DACGLE010000202          486 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0534
            SAMEA3304449-rid9560453.denovo.215, whole genome shotgun sequence.
ACCESSION   DACGLE010000202 DACGLE010000000
VERSION     DACGLE010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304449
            Sequence Read Archive: ERR985064
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 486)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 486)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702225.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702225.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 20:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,045
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 27 of 67
            Pseudo Genes (incomplete)         :: 25 of 67
            Pseudo Genes (internal stop)      :: 19 of 67
            Pseudo Genes (multiple problems)  :: 4 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..486
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304449-rid9560453.denovo.215"
                     /strain="VREN0534"
                     /db_xref="taxon:1352"
     gene            1..486
                     /locus_tag="IXT20_002887"
                     /pseudo
     CDS             1..486
                     /locus_tag="IXT20_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312603.1"
                     /note="internal stop; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
BASE COUNT          157 a           98 c           88 g          143 t
ORIGIN      
        1 ccatacttaa tgcgacgaca atggatttat tcttagcaca atcatcactc ctagaaatat
       61 tcatgacagt caagtcgcct ttcagctcat caaacaatcg aagagacttt ttcctgaaat
      121 caattgtgtt gtcgctgatg ctgagtataa gacacctaag tttgttcact ttttgactca
      181 tttaaacctt cgaccttgtt tgccttattc aaggcccaaa gggaaaaagg ggctattatc
      241 aaaaaatgag tttctctatg acgagtattt tgattgttat atttgtccgc aagatcaaat
      301 gctcgctttt tcaacggtta ctcgagaaag gtaccgagaa tacaaatcca atccaaaaga
      361 atgtgtgaac tgtcctctgc taaaccaatg taccaaaagt aaaaatcatc aacgagtcat
      421 cacgagacat gtttggggag atctgatgga tgaagtggaa catcttcgcc taacagatct
      481 gaataa
//
LOCUS       DACGLE010000203          485 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0534
            SAMEA3304449-rid9560453.denovo.216, whole genome shotgun sequence.
ACCESSION   DACGLE010000203 DACGLE010000000
VERSION     DACGLE010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304449
            Sequence Read Archive: ERR985064
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 485)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 485)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702225.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702225.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 20:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,045
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 27 of 67
            Pseudo Genes (incomplete)         :: 25 of 67
            Pseudo Genes (internal stop)      :: 19 of 67
            Pseudo Genes (multiple problems)  :: 4 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..485
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304449-rid9560453.denovo.216"
                     /strain="VREN0534"
                     /db_xref="taxon:1352"
     gene            43..249
                     /locus_tag="IXT20_002888"
     CDS             43..249
                     /locus_tag="IXT20_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4861252.1"
                     /translation="MVKFPQFKLNPAASIQFQGHVFDVLAKDNIKKYKKGNIFHIDIQ
                     ISSNLEDIKKAFKIKTIGTAPNFK"
BASE COUNT          211 a           62 c           50 g          162 t
ORIGIN      
        1 ggtaaaaaaa taaaaaaatg tgacaagtgt tttgaatcag ctgtggtaaa gtttcctcaa
       61 tttaaactaa atccagctgc ctcgatacaa tttcaaggac atgtatttga tgttttagct
      121 aaagataaca taaaaaaata caaaaaaggt aatatattcc atattgatat acaaatatct
      181 tctaatttag aagatataaa aaaagccttt aaaattaaaa caattggcac tgctcctaat
      241 tttaaataac aaaaaaatta ctagataatc taaaaacagg atggcattat gagaacaata
      301 actaaacttc ctatatcaca tattttgtaa ttagtgaaaa gcaaattgac tactatcata
      361 agttaatgac tctcatctta atatttaaaa tatattcata tttttctatt ttatctaaat
      421 gcgattttca tatctaataa tagaaaaata aacaattaac aataaaaaag tcttcattta
      481 ttgtt
//
LOCUS       DACGLE010000204          485 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0534
            SAMEA3304449-rid9560453.denovo.217, whole genome shotgun sequence.
ACCESSION   DACGLE010000204 DACGLE010000000
VERSION     DACGLE010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304449
            Sequence Read Archive: ERR985064
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 485)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 485)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702225.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702225.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 20:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,045
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 27 of 67
            Pseudo Genes (incomplete)         :: 25 of 67
            Pseudo Genes (internal stop)      :: 19 of 67
            Pseudo Genes (multiple problems)  :: 4 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..485
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304449-rid9560453.denovo.217"
                     /strain="VREN0534"
                     /db_xref="taxon:1352"
BASE COUNT          165 a           65 c           82 g          173 t
ORIGIN      
        1 taacgtgatt ttttgctgta atgacaaggt tttagctgtt tcagatacca tgtatccaaa
       61 aatagtttat tgagaaaata ggagagtatt tgatactaac tagtaggaaa aaatgagata
      121 aaaagtaaat ggaatcattt aaggtaatct gaaataaaaa atatacagcc ctgtggtact
      181 ttacctacaa ggctgtatat attttttact ctcagcgttt ttgactccgt gtctgactcc
      241 gttttattga ttttgaatgt tattcaatga ccatttaaca ggtcaaattt gatgtttttg
      301 attccgtatg acgccgtttg atatctagtg atatagtgat ttgagtctac atcgaataag
      361 ggaataaacc aataaaaaat gatttagaat attaaaataa caaaaaagag gactacctgt
      421 attagtcctc ttttttgacc agattttgac cagtctattt tacatcctag tatatttata
      481 tgagc
//
LOCUS       DACGLE010000205          467 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0534
            SAMEA3304449-rid9560453.denovo.218, whole genome shotgun sequence.
ACCESSION   DACGLE010000205 DACGLE010000000
VERSION     DACGLE010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304449
            Sequence Read Archive: ERR985064
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 467)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 467)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702225.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702225.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 20:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,045
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 27 of 67
            Pseudo Genes (incomplete)         :: 25 of 67
            Pseudo Genes (internal stop)      :: 19 of 67
            Pseudo Genes (multiple problems)  :: 4 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..467
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304449-rid9560453.denovo.218"
                     /strain="VREN0534"
                     /db_xref="taxon:1352"
     gene            7..>467
                     /locus_tag="IXT20_002889"
     CDS             7..>467
                     /locus_tag="IXT20_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010726162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAQ4861253.1"
                     /translation="MQTLVTKGDLNELFRSHLEKAINTLLRTELTAFLDYEKYDRTGF
                     NSGNSRNGSYFRSIKTEYGELTLEIPRDRNGEFKQQTLPAYKRTNDTLETTIIHLFEK
                     GVTMSEIADLIEKMYGHHYTPQTMSNMTKVLTEEVNAFKSRALNDKYVAIF"
BASE COUNT          171 a           84 c           77 g          135 t
ORIGIN      
        1 gaaattgtgc aaactctagt cactaaaggc gatttaaatg aattattccg ttcgcactta
       61 gaaaaagcga taaacacact cctacggact gaattaacgg cttttttaga ttacgaaaaa
      121 tatgatcgca ctggttttaa ttcaggtaat tcgagaaacg gttcttactt tcgatcaatc
      181 aaaaccgaat atggtgaatt aacattggaa atacctagag atcgtaatgg tgagtttaaa
      241 caacaaactt taccagccta caaaagaaca aacgatacat tggaaaccac tattatccat
      301 ttattcgaaa aaggtgttac gatgtctgaa attgctgatt tgatcgaaaa aatgtacggt
      361 catcactata ctccacaaac catgtccaac atgactaaag ttctgactga agaagtaaat
      421 gcctttaaat ccagagcctt aaatgataag tatgtcgcta tttttat
//
LOCUS       DACGLE010000206          463 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0534
            SAMEA3304449-rid9560453.denovo.219, whole genome shotgun sequence.
ACCESSION   DACGLE010000206 DACGLE010000000
VERSION     DACGLE010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304449
            Sequence Read Archive: ERR985064
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 463)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 463)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702225.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702225.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 20:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,045
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 27 of 67
            Pseudo Genes (incomplete)         :: 25 of 67
            Pseudo Genes (internal stop)      :: 19 of 67
            Pseudo Genes (multiple problems)  :: 4 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..463
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304449-rid9560453.denovo.219"
                     /strain="VREN0534"
                     /db_xref="taxon:1352"
     gene            complement(<1..>463)
                     /locus_tag="IXT20_002890"
     CDS             complement(<1..>463)
                     /locus_tag="IXT20_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="relaxase"
                     /protein_id="HAQ4861254.1"
                     /translation="GELITLGRDKQSRKEKSQYVERTFSARKLGKRYSFESVSEQLEK
                     NLEESHIKNFFNMEREQVMSLISGVNYKTGELNVPFVELDSIAQKQFSDREGDEKFIK
                     YDDFLREVDKHFVNTMSLEDDQEYTIKLHTDVNPQTLRSAKSKTISIKKTDQ"
BASE COUNT          132 a           90 c           56 g          185 t
ORIGIN      
        1 ctgatcagtc tttttaatag aaattgtttt acttttcgca ctacgtaaag tttgaggatt
       61 cacatccgta tgaagtttaa tcgtgtattc ttgatcatct tctaaagaca ttgtatttac
      121 aaaatgcttg tctacctctc gcaagaagtc atcatattta atgaattttt catcgccttc
      181 acggtcgcta aattgctttt gtgcgatgga atctaattcc acaaacggta catttaactc
      241 accagttttg taatttactc cactaattag cgacataact tgctctcttt ccatattaaa
      301 aaaatttttt atatgtgatt cttctaaatt cttttcaagt tgttcagaaa cagattcaaa
      361 tgaatatctt ttccctaact ttcttgcact aaaagtacgt tcgacatact gacttttttc
      421 ttttctagat tgtttatctc ttcctaaagt aattaactca cct
//
LOCUS       DACGLE010000207          460 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0534
            SAMEA3304449-rid9560453.denovo.220, whole genome shotgun sequence.
ACCESSION   DACGLE010000207 DACGLE010000000
VERSION     DACGLE010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304449
            Sequence Read Archive: ERR985064
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 460)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 460)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702225.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702225.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 20:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,045
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 27 of 67
            Pseudo Genes (incomplete)         :: 25 of 67
            Pseudo Genes (internal stop)      :: 19 of 67
            Pseudo Genes (multiple problems)  :: 4 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..460
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304449-rid9560453.denovo.220"
                     /strain="VREN0534"
                     /db_xref="taxon:1352"
     gene            complement(20..91)
                     /locus_tag="IXT20_002891"
     tRNA            complement(20..91)
                     /locus_tag="IXT20_002891"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(56..58),aa:Glu,seq:ttc)
     gene            complement(113..200)
                     /locus_tag="IXT20_002892"
     tRNA            complement(113..200)
                     /locus_tag="IXT20_002892"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(164..166),aa:Ser,seq:gct)
     gene            complement(225..298)
                     /locus_tag="IXT20_002893"
     tRNA            complement(225..298)
                     /locus_tag="IXT20_002893"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(262..264),aa:Ile,seq:gat)
     gene            complement(312..382)
                     /locus_tag="IXT20_002894"
     tRNA            complement(312..382)
                     /locus_tag="IXT20_002894"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(348..350),aa:Gly,seq:tcc)
     gene            complement(384..459)
                     /locus_tag="IXT20_002895"
     tRNA            complement(384..459)
                     /locus_tag="IXT20_002895"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(424..426),aa:Asp,seq:gtc)
BASE COUNT          132 a          128 c          108 g           92 t
ORIGIN      
        1 taaaactata ttaaattaat gacccgtacg ggattcgaac ccgtgttacc gccgtgaaag
       61 ggcggtgtct taaccgcttg accaacgggc caggtaaaac aaaatattga aacggagaag
      121 gagggatttg aaccctcgcg ccggtttccc gacctacacc cttagcaggg gcgcctcttc
      181 agccacttga gtacttcccc aaaaccaata agattttgta ctaatgggcc taaatggact
      241 cgaaccatcg acctcacgct tatcaggcgt gcgctctaac cagctgagct ataggcccat
      301 aaaaaactaa aagcgggtga cgagaatcga actcgcgaca acagcttgga aggctgtggt
      361 tttaccacta aactacaccc gcatggcggt tccgacggga atcgaacccg cgatctcctg
      421 cgtgacaggc aggcatgtta acccctacac cacggaacca
//
LOCUS       DACGLE010000208          454 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0534
            SAMEA3304449-rid9560453.denovo.221, whole genome shotgun sequence.
ACCESSION   DACGLE010000208 DACGLE010000000
VERSION     DACGLE010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304449
            Sequence Read Archive: ERR985064
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 454)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 454)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702225.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702225.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 20:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,045
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 27 of 67
            Pseudo Genes (incomplete)         :: 25 of 67
            Pseudo Genes (internal stop)      :: 19 of 67
            Pseudo Genes (multiple problems)  :: 4 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..454
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304449-rid9560453.denovo.221"
                     /strain="VREN0534"
                     /db_xref="taxon:1352"
     gene            complement(116..400)
                     /locus_tag="IXT20_002896"
     CDS             complement(116..400)
                     /locus_tag="IXT20_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAQ4861255.1"
                     /translation="MNQMKNEKDQPFDPDYLNRCMKTVVKENKEIREITIHGFRHTHC
                     SLLFEAGATLKEVQDRLGHANSDITLKIYTHVTTEAKRETANKLALYLDS"
BASE COUNT          123 a           68 c           74 g          189 t
ORIGIN      
        1 attgtgtttt atgtgtctta cgtttgttta atatgtatgg aaagccttta ataggtgaat
       61 agaaaattta tttttgttga actaaaaaag aagctaattc gtatgaacta gcttcttagc
      121 tatctaaata taaagcaagt ttatttgctg tttctctttt agcttcagtt gtgacgtgag
      181 tataaatctt caaagttata tcactattag catgacctag acggtcttgg acttctttca
      241 atgttgctcc tgcctcgaat aacaatgaac aatgagtgtg tctaaaaccg tggattgtga
      301 tttctcgtat ttctttattt tctttaacaa cagttttcat acaacgattc aaatagtcgg
      361 ggtcaaatgg ttggtctttt tcattcttca tttgattcac ctcttttcct tttttatttt
      421 tacgaggatg attataccat ttttggttat ctaa
//
LOCUS       DACGLE010000209          453 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0534
            SAMEA3304449-rid9560453.denovo.222, whole genome shotgun sequence.
ACCESSION   DACGLE010000209 DACGLE010000000
VERSION     DACGLE010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304449
            Sequence Read Archive: ERR985064
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 453)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 453)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702225.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702225.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 20:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,045
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 27 of 67
            Pseudo Genes (incomplete)         :: 25 of 67
            Pseudo Genes (internal stop)      :: 19 of 67
            Pseudo Genes (multiple problems)  :: 4 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..453
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304449-rid9560453.denovo.222"
                     /strain="VREN0534"
                     /db_xref="taxon:1352"
     gene            93..422
                     /locus_tag="IXT20_002897"
     CDS             93..422
                     /locus_tag="IXT20_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAQ4861256.1"
                     /translation="MITKTRKQGNSIMLTVPKDFNVPNGVEVEAKLVENGILYEFVEP
                     QKEFYDFSEDILSDIIAEGYDKDEILVEFKNRKNKMHSSFRDIAEDTLTNSKVMTKEE
                     LATEIGL"
BASE COUNT          174 a           53 c           81 g          145 t
ORIGIN      
        1 ttacgagtca cagtaattac ttgtaaatac attatctcgt ggtataatgt atttactaag
       61 taattacata ctgttagaaa tgagggattt aaatgattac aaaaacaaga aaacaaggca
      121 attcaatcat gttgacagta cctaaagatt ttaatgttcc taatggagtc gaagttgaag
      181 caaagttggt agagaatggt attctttatg aatttgttga accacaaaaa gaattttatg
      241 attttagcga agatattttg tctgatataa ttgcagaagg ctacgataaa gatgagattc
      301 ttgtagaatt taagaataga aaaaacaaaa tgcattcttc ctttagagat attgctgaag
      361 atacacttac taattctaaa gtaatgacta aagaggagtt ggcaacagaa attggcttat
      421 gaaatcgttc cttcaaaaca tgtcattaag tat
//
LOCUS       DACGLE010000210          446 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0534
            SAMEA3304449-rid9560453.denovo.223, whole genome shotgun sequence.
ACCESSION   DACGLE010000210 DACGLE010000000
VERSION     DACGLE010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304449
            Sequence Read Archive: ERR985064
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 446)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 446)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702225.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702225.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 20:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,045
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 27 of 67
            Pseudo Genes (incomplete)         :: 25 of 67
            Pseudo Genes (internal stop)      :: 19 of 67
            Pseudo Genes (multiple problems)  :: 4 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..446
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304449-rid9560453.denovo.223"
                     /strain="VREN0534"
                     /db_xref="taxon:1352"
     gene            11..>446
                     /locus_tag="IXT20_002898"
     CDS             11..>446
                     /locus_tag="IXT20_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ4861257.1"
                     /translation="MRKYTFVFKKKVVSDYLNNEGGYKYLAHKYQINRTLVRHWVRIY
                     NYHGWEGLVGGGKSYTTKFKLDVIEYMETNGLSIQETAKKFNIGSNRTLSKWIEQYEE
                     GGASSLESQKRGRKISMNSKLNIPKKLKDESLEEEVIRLRAEN"
BASE COUNT          171 a           58 c           90 g          127 t
ORIGIN      
        1 ggtgcttttt ttgcggaaat atacatttgt ttttaagaaa aaagtagttt cagactattt
       61 aaataacgaa ggcggctaca aatatcttgc acataaatat caaataaatc gtacactggt
      121 tagacattgg gtaaggattt ataactatca tggttgggaa ggcttggttg gaggtggcaa
      181 aagctacact acaaaattta aacttgatgt tatagaatat atggaaacaa atggtctttc
      241 tatccaagaa actgctaaaa aatttaatat cggttcaaat agaactctaa gtaaatggat
      301 agagcaatat gaagaaggcg gtgcttcttc acttgagagc caaaaaaggg gcagaaaaat
      361 tagtatgaat tccaagctaa acattcctaa aaaacttaaa gatgagtctc ttgaagaaga
      421 agttattcgt ttaagagcag agaacg
//
LOCUS       DACGLE010000211        49390 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0534
            SAMEA3304449-rid9560453.guided.013, whole genome shotgun sequence.
ACCESSION   DACGLE010000211 DACGLE010000000
VERSION     DACGLE010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304449
            Sequence Read Archive: ERR985064
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 49390)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49390)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702225.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702225.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 20:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,045
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 27 of 67
            Pseudo Genes (incomplete)         :: 25 of 67
            Pseudo Genes (internal stop)      :: 19 of 67
            Pseudo Genes (multiple problems)  :: 4 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49390
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304449-rid9560453.guided.013"
                     /strain="VREN0534"
                     /db_xref="taxon:1352"
     gene            195..932
                     /locus_tag="IXT20_002899"
     CDS             195..932
                     /locus_tag="IXT20_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase"
                     /protein_id="HAQ4861258.1"
                     /translation="MKIFRRILIGLVIILGLLGGAGYYYIQQNTYEPSSEAQVLVKEG
                     KNKEDYLFFEGEKKNHGKSPLLIFYPGALVEPESYSIWAQDLAKNGFSVAIVKMPLDL
                     AVMGGNKADKVKEDFPNSDYVIGGHSLGGVMASRYVNKNHSDKHLKGVFFMASYPDKK
                     GTLTESAVPVLSITATKDKVLNQEAYQEAKQYLPERTEYVSINGGNHGGFGSYGEQKG
                     DGEAEISNADQQTVIVNTLENWLKKLK"
     gene            complement(1097..1318)
                     /locus_tag="IXT20_002900"
     CDS             complement(1097..1318)
                     /locus_tag="IXT20_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HAQ4861259.1"
                     /translation="MSLLCKLVPERCIACGLCQIEAPDIFDYDEEGIVLFAQEPEAHQ
                     QFVMPEEETYVKKAYQKCPVRAILLENKI"
     gene            1349..2380
                     /locus_tag="IXT20_002901"
     CDS             1349..2380
                     /locus_tag="IXT20_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4861260.1"
                     /translation="MIVFILSLFSSGHKLRISSLYQLLVGKRTTSVLIYGFTHELLFI
                     HNSFPELKQDKFYQILQKIAQQGWIEINENEAKLTPAGADMLSEHQIEHTGLRFDRYG
                     RTGETSWRLIKFAVQVISNLATGIQDYLPAETSPFYTFQLKKWLSESQLPREILIDTA
                     YESLVQLFSEIPEEAADFLANQLSGNERTGLLPYQLAKNNDESAVYLQQSRCIHLLLA
                     QIEKRPDSLWHALIDSLLQQNFNQSMMITKQMFMNGQTIDQIMAIRHLKRGTVTDHLI
                     EWALFFDDFPYEWILSAETIERLEPNKDSVREWRFSEWNVDGQLDYGEFRLYQIYLLR
                     KEAIQNVNK"
     gene            2367..3770
                     /locus_tag="IXT20_002902"
     CDS             2367..3770
                     /locus_tag="IXT20_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HAQ4861261.1"
                     /translation="MLINELKKYFGFDSFRPGQEEIIQALLDGEDTLAILPTGTGKSL
                     CYQLTGYLMEGLVIIVSPLLSLMEDQVTQLQKRGEKRVAAFNSLLSQSERRYVLRHLS
                     QYKFLFLSPEMLTNPSLLEHLKKQNIALYVVDEAHCVSQWGVDFRPEYQQLGKIRKLL
                     GNPVTLALTATATDLVARDIRHVLFDRPPKEIRQSVNRQNISLFVRKTQQKEQELEHF
                     MERAQGAAIIYCATKKEVERLYHLFRERFTVGYYHGGLDAAQRRQLQQQFVKKQLQFL
                     IATNAFGMGIDKSDIRYVVHYDLPDSLENYVQEIGRAGRDQEESAAILFYQSGDERIH
                     YFFNQLSREQRQSFELYLEYATEQAPFDELQKKWMELIQQSEKPENWVERLKRQEKEK
                     EFRLQQMLRYINEENCRRKFILAYFGEKLSEKPQNCCDIDGAKAELLPKRKLFSKTEQ
                     LHWETILLNLFKKNMEH"
     gene            3836..4471
                     /locus_tag="IXT20_002903"
     CDS             3836..4471
                     /locus_tag="IXT20_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAQ4861262.1"
                     /translation="MSRKDRHQSTENNETQEPWEQPIYDTDDEVSSRSSQRQQKKGNT
                     LFLSLFLILLVLCIAIPAGAYFWIRNGSSNNATAATSSETSTSVVESSTSTTKEESTE
                     TSTVESSTIAEESTVSQDTGVTSESSIAAETTPTSSSEATYQAGDSSSNAAETTPSSS
                     AAAGSTTTVQAGEGPKQVAERAGITTDQLFELNGLDPNNFMLYPGQELRIK"
     gene            4520..5200
                     /locus_tag="IXT20_002904"
     CDS             4520..5200
                     /locus_tag="IXT20_002904"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HAQ4861263.1"
                     /translation="MTQISIAIDGPASSGKSTVAKILAKELNYIYTDTGAMYRAVTYL
                     AITNNVSFSDEEGLVSLIKKYPISFSQQEDGQHVFIASKDVTLEIRQPDVTKAVSEVS
                     AHGKVREELVAVQRKIGERGGVVMDGRDIGTAVLPNAEVKIFLVASVSERAERRFKEN
                     QEKGIPTDLETLTKEIQERDHYDSTRDVSPLKQAEDAVRIDTTGKSIPEVVAAIKAVV
                     LKKGYLLF"
     gene            5335..6564
                     /gene="rpsA"
                     /locus_tag="IXT20_002905"
     CDS             5335..6564
                     /gene="rpsA"
                     /locus_tag="IXT20_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HAQ4861264.1"
                     /translation="MTEFDQNQENNQSMEDAMKSVQEVKVGDVVKGEVLVIEDKQVIV
                     GIEGAGVEGVVPAKELSTLPVEDISEVVKVGDVLDLVVISTIGKDKENGSYLLSKRRL
                     DAKKVWEDIERDFQEGKVIEAPVTNVVKGGLVVDVGVRGFVPASMVEDHFVADFSDYK
                     GKTLSFKIIEIEPSENRLILSHKAVVENEREEKKKEILSTIHEGDVLSGRVARLTDFG
                     AFVDLGGIDGLVHVSEISHSHVAKPSDVLAVNDDVNVKVLSINPEQERVSLSIKDTQP
                     GPWSEIDTKAPVGTVLDGTVKRLTSFGAFVEVFPGVEGLVHISQISHKHIATPHEVLH
                     EGDEVKVKVLEIHPEEHRIALSIKALEEKPASEEEPKNVESYELPEENTGFTMGDILG
                     DALKEEVEEKATDDSEK"
     gene            6746..8056
                     /locus_tag="IXT20_002906"
     CDS             6746..8056
                     /locus_tag="IXT20_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HAQ4861265.1"
                     /translation="MANPTIAIVGRPNVGKSTIFNRIAGERISIVEDTPGVTRDRIYA
                     KGEWLGREFSVIDTGGIDLGDEPFMDQIKHQAEIAIEEADVIICVVSGREGVTDADEM
                     VAKILYRSNKPVILAVNKVDNPEMRNDIYEFYSLGLGDPYPVSGSHGLGIGDILDEAV
                     KYFSEETEEEDDDTIKFSLIGRPNVGKSSLINAILGEERVIVSDIEGTTRDAIDTHFV
                     SESGQKFLMIDTAGMRKRGKVYENTEKYSVMRAMRAIDRSDIVLMVLNAEEGIREQDK
                     RVAGYAHEAGRGIIIVVNKWDLVKKETNTMRDFEQEIRDEFRYLDYAPIVFVSAVTKQ
                     RLERLPEMIEQVSMNQNLRISSAVLNDIIMDAVAINPTPTDKGKRLKIFYATQVAVKP
                     PTFVVFVNEEELMHFSYERFLENQIRKAFTFEGTSIRIIPRRRK"
     gene            8299..8574
                     /locus_tag="IXT20_002907"
     CDS             8299..8574
                     /locus_tag="IXT20_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008214712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HAQ4861266.1"
                     /translation="MANKAELIEKVASATDLTKKDATAAVDAVFSTIQDALANGEKVQ
                     LIGFGNFEVRERAARKGRNPQTGEEIEIPASKVPAFKPGKALKDAVK"
     gene            8809..9855
                     /locus_tag="IXT20_002908"
     CDS             8809..9855
                     /locus_tag="IXT20_002908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4861267.1"
                     /translation="MELDYQVEDALTKVFYGETLVSQLRELSIDQPILFLTNQRYYDL
                     FADKINPLFANQANNDWYICANTQCNHLTELKNLLDFVKRYPENQSMFIIGFGNEGIM
                     DLAGFFQKHTTLDSSLWMIPVSIRSLSRALTPQRTILQLPNRVVLQTINLPERIVYDQ
                     TISERQVDGKQIDFLTFICCGMICDHRFLQNLYKNYPNKKQLTTRPFAGMIEEMIYFY
                     QLEAENLSNYGKLFEQAFYLTENGHLLSASMKKFLGMMFHFVWNLQAYPFDFQLKNFF
                     VWLDLLGYPIHWLEQISKMEYLEKVLFLAEKSKKILVLEKIGIIDGYQHPNEKELLRT
                     MTVFQQIVSEIRGN"
     gene            9857..11116
                     /locus_tag="IXT20_002909"
     CDS             9857..11116
                     /locus_tag="IXT20_002909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HAQ4861268.1"
                     /translation="MNTNSEKMLQALSDEDLSQAQIFLEKALKEDPADILAELGEELL
                     AIGFLIEAKQIFEQLINQYPENDGLNIPLAEIAIEDNEIDLAFEYLEKVDKQSDYYPQ
                     SLLAIADLYQVIGIPEVSEAKLKEAAGLLPDEPLIQFALAELYFSVDRFKEAAVVYET
                     LLASGINEISGISMQERLGQSLSMQGEFEAAIVPLEEALKEERTDDRLFQLAFTNLQL
                     KENQQAINYLEELREVNPHYQSLYLYLGQALQEEEMIEEAQTVLEEGIKENPYQVELY
                     HLASENAFRLHDRQRAEELLLKALEVGDKQDETLLTLSNLYLDDERYEDVIQVINQME
                     ETANPYAEWNLAHAYNELEDFTVAAVHYEQAYHELSHEPDFLKEYAFFLREEGQLKRA
                     QELLTYYLTLEPGDMEALSLLDDLTER"
     gene            11122..11703
                     /locus_tag="IXT20_002910"
     CDS             11122..11703
                     /locus_tag="IXT20_002910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IDEAL domain-containing protein"
                     /protein_id="HAQ4861269.1"
                     /translation="MLIDVREKKNFLTWMVNHVSFSRREVFWILNYLANHEAILSNVH
                     FVEGANFADRGLQISDLSVEGEPMKLFLQGKEFTDTDQIFHEIRLNWKKPLYVECIFE
                     NAWQTREYLSILEDNPFSSWDSSVSEEDTELIERYFQEKEEEAKLKMLYAQIDQALED
                     GDRDAFLKLSDEVNRKILLQTQKKSNRVSEHNN"
     gene            complement(11785..12660)
                     /locus_tag="IXT20_002911"
     CDS             complement(11785..12660)
                     /locus_tag="IXT20_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAQ4861270.1"
                     /translation="MVKRKTVKEVLFIIIGTSIYAFGLVYLNIANHLAEGGVSGITLI
                     LRALFGIDPAYTTLLINIPLILLGGKILGKRSLAYTVLGTLSLSFFLWIWQRVPLEIN
                     LQHDLLIVSLLAGLVAGVGSGIVYRMGGTTGGSDIIARILEQNYGITMGRSLLAFDII
                     VLLASLTYIDLKRMMYTLIVSYVFSRVIDSILDGGYSAKGLLVVSNKSEEIAPLLMTG
                     LQRGVTFLSGEGAFSGNSKKIIYMVVSSSELTEAKRIVHEIDEKAFLSIINVHEVEGQ
                     GFTYLKPQTRLLKKL"
     gene            12808..13140
                     /locus_tag="IXT20_002912"
     CDS             12808..13140
                     /locus_tag="IXT20_002912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HAQ4861271.1"
                     /translation="MDKKRSLYSMQQEVDEYIQQFKVGYFSPLAQMARLTEEVGELAR
                     EVNHTYGEKSKKASEPVNSVAEELGDVLFVTMIMANSLNIDLTEVFEKNMEKFNQRDH
                     NRFERKDE"
     gene            13252..14460
                     /locus_tag="IXT20_002913"
     CDS             13252..14460
                     /locus_tag="IXT20_002913"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HAQ4861272.1"
                     /translation="MKLKQLPLEYQKAIPVLKKLENAGYEAYFVGGSVRDILLNQPIH
                     DVDIATSAFPEEIKQLFPKTIDIGIEHGTVLVLENDEQYEITTFRTESTYQDYRRPDH
                     VEFVRSLAEDLKRRDFTINAFALKEDGEIIDLFDGLQDLEEQILRAVGNPHERFHEDA
                     LRMMRGLRFVSQLGFKLEEETFYAIKENHELLGKISVERINVEFIKMLLGNERAAGIH
                     AFIETECYICCPGLKEEGEALLRFAELPSVPLKEESQAWALLISQLDLKENEIRSFLK
                     SWKCSNQMIKDVQALVQGLRQRETGALEPFELYFLGKKHALQVEELMIYFDETPQMDA
                     VGESYDRLPIKSLSELAVDGKILLEETGKKPGKWVSEALQLAEKAVVEQRIKNDKKQV
                     LDFLSKEKLI"
     gene            14614..15069
                     /locus_tag="IXT20_002914"
     CDS             14614..15069
                     /locus_tag="IXT20_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4861273.1"
                     /translation="MSKEMTALKFYFRNGETWTINRRHIGDLWIKQITTSFGRINGSE
                     FVEIHPCAGFKIEIFHEGDAVATHDINLGGLEMGMFNRALKYEDIERMEILYRNGTPD
                     LVYFPYLDKGTEGLDNQYQSTKISEKTGNLYIVINPDQRVEDVYGEFFE"
     gene            15431..15706
                     /locus_tag="IXT20_002915"
     CDS             15431..15706
                     /locus_tag="IXT20_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4861274.1"
                     /translation="MRESFEQQKKLLHDRYGALSMDDRRQILCKLRKRNILMYRQLER
                     LKHDLLRLESKRVQCELEGNQTQVEVVETKILKKKEQFLKMLTQNKK"
     gene            15719..17797
                     /locus_tag="IXT20_002916"
     CDS             15719..17797
                     /locus_tag="IXT20_002916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HAQ4861275.1"
                     /translation="MEFAELVIVFAVTITLSNIASRILPMIPAPLIQIFLGVVLGLTE
                     WGQSIDFEPELFLVMIIAPLLFREGEKADISSILKNFGTILFLAFGGVILTLVGVGAT
                     LSFLLPSVPLAACFAFGAALGPTDAVAVSSLSGRVNIPKKAMHILEGEGLLNDASGVT
                     AFQFALGALITGSFSAVNAGMSLVVSSIGGALIGFLLVWFKQKIIHLIEKASAQDVTG
                     YLLIELLLPFLAYVLAEAFDVSGIIAAVAAGILQASGFRKISVFDAELSSLSHSTWTT
                     IAFTLNALVFIFLGIELTQVFSPVWGDGLYPNGLLLAIIVLISVMLFVIRFISISLFY
                     VFKDGSKKFKKQLNEILILTFGGVKGTVSLATIFILPFSINNMMFYQRSLLLFLTAGV
                     ILVTLVIGIIVLPMLTETEEAKSTDLNALMILEEVVEVLRKEIKEIDRNTKEFLATEA
                     VIENYQERIRDLYLEDLTDDEKQEVQEIQALILSIERDGLDESYRSGKLSSNGYRFYS
                     RFLSRFEHSITSQILSFIGFWFIVVRRLMRIVLHPKMFWQRRYADRQTFISDEDIQEI
                     RETYQKNTQLIIESLDNLTDIYDETMVQFFIQQRKLEGIKMNSGNLISSWMIQQDSLF
                     TKKMLRGYYLERKIIDEYEVAEKITTFSANSYRRNINLLESYTMNKPSDNFSFRFAFR
                     AKSKKTSPIK"
     gene            17957..19843
                     /locus_tag="IXT20_002917"
     CDS             17957..19843
                     /locus_tag="IXT20_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAQ4861276.1"
                     /translation="MLKELKVTDLKKTYGEKDLFDQISFLVHEKDRIGLIGTNGTGKT
                     SLLNIIAGIDSSDGDRQTVFYPTDYRIGYLSQAAEFSDELTVLQAVFQGNSPLIQTVR
                     AYEEALIELGENGEDPDVQKRYAKAEEQMNKEDAWTTDTNAKIILQKLGISELDKKIS
                     TLSGGQKKRVSLAQVLIDEPDLLLLDEPTNHLDYEAIEWLENYLKQYRGALLMVTHDR
                     YFLDRVANRIFELSFGKLYEYKGNYEAYVLEKAERDRVEVEQEEKRKRLYKQELAWMR
                     AGVQARGTKQQARINRFEDLKENLYQVNQEDDLELNLATQRLGKKVLEIKDGSYRIND
                     QTLLEHLDLLIQSRERLGITGKNGAGKSTLLNILAGRIPLDSGTMSIGETVHLAYYTQ
                     ENEEMAPDKRMIAYLQEAAEEAKTADGSQIGVAELLERFLFPRFMHGTLIGKLSGGEK
                     RRLFLLKLLIQQPNVLLLDEPTNDLDIATLTILEDYFRSFPGAVITVSHDRYFLDKVA
                     DKLLVFQGNGKQELYYGNMSSYLLKQKETQQPAEKAKPKIQSKEPAEKKKLSYMEQKE
                     WETIEDEIAELEEKISLLQEEMNHQGDNFTRLQELQNDVSETEAQLEEKMARWEYLSE
                     WVED"
     gene            19855..20802
                     /locus_tag="IXT20_002918"
     CDS             19855..20802
                     /locus_tag="IXT20_002918"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HAQ4861277.1"
                     /translation="MEQAYLDLGRKLLEEGHRKEDRTGTGTLSLFGYQMRFDLQKGFP
                     LLTTKRVPFGLIKSELLWFLKGDTNIRYLLQNKNHIWDEWAFERYIKSPDYNGPDMTD
                     FGHRCLTDPEFNEEYKKQSKLFCEKILTDDSFAETYGDLGHIYGYQWRHWETKDGGFI
                     DQIKQVIEAIKKTPDSRRLIVSAWNPEDVPSMALPPCHTMFQFYVQEGRLSCQLYQRS
                     GDVFLGVPFNIASYALLTHLIAHETGLAVGEFVHTLGDAHLYINHIEQMKEQLSREIR
                     AFPTLVLNQEKQSVFDFEMEDIQLEGYDPHPAIKAPIAV"
     gene            20822..21349
                     /locus_tag="IXT20_002919"
     CDS             20822..21349
                     /locus_tag="IXT20_002919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAQ4861278.1"
                     /translation="MLAAIWAQDENGLIGKEDRLPWRLPNDLKFFKQMTEANTLVMGR
                     KTFEGMGSRPLPNRQTIVLTRDSSYKAEGVHVMHSVEDVLALEKETDGIFFIAGGSAV
                     YQEFLPFCTILYRTVIHHVFDGDAYFPPVDWSDWSLINTSQGEMDEKNRYPHQFETYQ
                     RNENPVKSGTGELTI"
     gene            complement(21412..22065)
                     /locus_tag="IXT20_002920"
     CDS             complement(21412..22065)
                     /locus_tag="IXT20_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HAQ4861279.1"
                     /translation="MEKTKFNRTYEILNEVFNAVTHGIGTGLSIAGLVVLLVKGAHLH
                     SSLHIVSYAIYGSMMILLFLTSTLFHSLIFTKAKKVFQVFDHSSIFLLIAGSYTPFCL
                     LSIRGWLGWTLFVLIWLFAILGIIYKALTLHKRERVSKVSTIIYIFMGWLCVVAAVPL
                     YHSLGMTGVILMVAGGLSYTIGAFFYSLNSIRYMHVVWHLFVMLGAGFMYFSVLFFT"
     gene            22197..23039
                     /locus_tag="IXT20_002921"
     CDS             22197..23039
                     /locus_tag="IXT20_002921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAQ4861280.1"
                     /translation="MTNVKIVTDSSCTMEKNVRDALGIRVMPLSIMIDGTVYPDDDQL
                     DGERFMEMMAKAKNLPKTSQPPIGEFISMYDELGEDGSEVISIHMTKGLSGTVEAARQ
                     ASNLTKTKVTVIDSDTTDQGLSFQVIRAAELAKEGKTVEKILEEVKKVQDNTKLYIGI
                     STLDNLVKGGRISRTTGLLSSVLNIRVVMNFAHSELIPVTKGRGKKTFDKWFNNLKNE
                     LKELPNVRQIGISHADAKELAQEFKEGLQELFPDMHIPVLHTNPVIATHTGKDAFAIM
                     YYVD"
     gene            23197..24093
                     /locus_tag="IXT20_002922"
     CDS             23197..24093
                     /locus_tag="IXT20_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HAQ4861281.1"
                     /translation="MKLTKRHLILVLFPLLIAIIIYAGLAASIPKAGPLMKPVTTVRE
                     DPNKKEKLHYIAIGDSLTEGVGDQTKQGGFVPLVANDLKDRYNLTAVETENYGVNGER
                     SDQILKRVKTKEEIRNNIKTADFITLTVGGNDLMKVIQNNLFGLTVNSFKKPLKNYQE
                     NVTELLEEFRSLNKTAPIYILGIYNPFYLNFPEITDMQTIVNNWNKGTETVTKKMPNC
                     YFIPINDLLYQGLNQEVGVTEEGTDQTTSTTENLNGIKNNVLYEEDHFHPNNLGYQLM
                     ANAVRDQLIETQNQWLEKEDDQ"
     gene            24096..24809
                     /locus_tag="IXT20_002923"
     CDS             24096..24809
                     /locus_tag="IXT20_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpmS family protein"
                     /protein_id="HAQ4861282.1"
                     /translation="MKTRSDVSSNKTKKTNYWKFAFLILLGVVLGSTVFLGSRIFANR
                     EPELPEIPALTERQGDPVLTINSNKEKVNQIISFFLSEYQKDSDIEYKFYLENEALLN
                     GTFEVLGFPIDFYLYFDPYVMENGNVQLKARSLSIGTLSLPIKDVMNMIKRNYKLPDW
                     IEVNTDDLTIMLRLDQFRMQNGMYIKADKIDLVNDDIRFSLYLPKDTSDASNNSNTNK
                     TSTSSTESKTKENKKSSDE"
     gene            24829..25347
                     /gene="msrA"
                     /locus_tag="IXT20_002924"
     CDS             24829..25347
                     /gene="msrA"
                     /locus_tag="IXT20_002924"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HAQ4861283.1"
                     /translation="MERAIFAGGCFWCMIQPFDTLPGIHTVMSGYTGGHVPDPTYEQV
                     KSQTTGHTEAVEIIYDPTLISYEQLVELYWQQTDPTDAFGQFEDRGDNYRPVIFYLTE
                     EQRIIAEKSKKQLAESGRFEQPIVTKIEPAQPFYLAEEDHQEYYKKHPENFKRNHARR
                     AAFIEENWRNEG"
     gene            25344..25571
                     /locus_tag="IXT20_002925"
     CDS             25344..25571
                     /locus_tag="IXT20_002925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HAQ4861284.1"
                     /translation="MNRSFFHYLMTLRGGGTHDALSVFATEAGKDIQFPKHSSSYEEI
                     SNYLELNVDYLPSMDIFDEAWEKYLENNHDI"
     gene            25723..26274
                     /gene="lepB"
                     /locus_tag="IXT20_002926"
     CDS             25723..26274
                     /gene="lepB"
                     /locus_tag="IXT20_002926"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HAQ4861285.1"
                     /translation="MTKKQRYINRFWLVFKYLLVSVFLAFMLRGFLFIPVPVEGNSME
                     NVLKQGDMVVMEKFSEIRRFDIVVFQLADGTIYIKRVIGLPGENVSYQNDQLKINGKV
                     VKEPYLTKNLKSDHANASYTTDFTLQELTGQSKLPEDNYFVLGDNRRVSKDSRSFGTI
                     NKTDILGKARFVYYPLDEIKWIQ"
     gene            complement(26357..26899)
                     /locus_tag="IXT20_002927"
     CDS             complement(26357..26899)
                     /locus_tag="IXT20_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HAQ4861286.1"
                     /translation="MNTLIAKDVFRLPEMNARLLSTSALLLALDVILYKIAIGPAYFQ
                     ITFGFLSMALMGYLYGPIWAGLLEMLSNTLNFTIFGSGTFQAAFLITAFLGGFINGLF
                     LYHKKINLPSVIAAQLLIMIPVSLFINSWLMSVLFGADFKVIFLGRFIRNMIQIPIQI
                     MVVYSFIKTLEARKVFKRAV"
     gene            complement(27136..27534)
                     /locus_tag="IXT20_002928"
     CDS             complement(27136..27534)
                     /locus_tag="IXT20_002928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HAQ4861287.1"
                     /translation="MNSMVFVNFPVADVNRSVAFYEKLGFKKNEEFSTEEAGSMVWDD
                     TFWIMLLSHDFYKKFIKDKEIADNKKTSGALISFSVENADEVKKFAQIAKENGGTFHE
                     VDMGMPEEEMFSLEVQDLDGNTLEPVWMKM"
     gene            27794..28654
                     /gene="ylqF"
                     /locus_tag="IXT20_002929"
     CDS             27794..28654
                     /gene="ylqF"
                     /locus_tag="IXT20_002929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HAQ4861288.1"
                     /translation="MTIQWFPGHMAKARREVSEKMKYVDIVFELVDARLPLSSRNPML
                     DQIIQQKPRLVLLNKADLADPQQTKLWQEYFRKQGHLALAITAQESKGIKGLVPAAKE
                     ALKEKRARDAARGIKPRAIRAMVLGIPNVGKSTLMNRLVGKKIAQTGNKPGVTKGQQW
                     LRLGNELELLDTPGILWPKFEDEEIGKKLALTGAIKDQLVHLDDLAIYGLDFFARYYS
                     GRISDRYGLSIEQEEKLAPELLMDITAKRGYKEDYDRGSELVVHDIRQGKLGRYTLDR
                     CEEMEQKSDD"
     gene            28647..29414
                     /locus_tag="IXT20_002930"
     CDS             28647..29414
                     /locus_tag="IXT20_002930"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HAQ4861289.1"
                     /translation="MTESIQSIKERLKSIQRIDDPTLESLRNDPRKGVQQALASFERR
                     LKKEALIKEKYEEMFCFEKQGYAQGHQWIAGIDEVGRGPLAGPVVAAAVILPKNEIIL
                     GLNDSKQLSEKKRELLYQQVQEKALAIGIGIVDQQTIDDVNIYEASKLAMIQAVEKLS
                     IVPSYLLIDAMTLDLPIQQEKIIKGDARSVSIAAASIIAKVYRDQLMKEYDVLYPGYG
                     FSHNAGYGTKEHLEGLERQGITPIHRKSFAPVKKYLQ"
     gene            29470..30327
                     /gene="dprA"
                     /locus_tag="IXT20_002931"
     CDS             29470..30327
                     /gene="dprA"
                     /locus_tag="IXT20_002931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HAQ4861290.1"
                     /translation="MYQIEENLLKLAYTKGISSQGKWALANWMMQYPYKEIGFEDIIK
                     IGKITKHRELFRKSWQEIHANWGKIQSKQSFITCFDPKYPPQLLHLTYPPIVLFYNGD
                     LSLLSCNMLSVVGGRHTSALAKKTVYYLLKPVIEAGYVIVSGCAKGIDTYGHQAAISH
                     GGRTIAVIGTGIEQVYPAENRDLQEVIANDHLLLSEYPPQSGPKKHCFPMRNRIIAAL
                     SKGTCVFEAKKKSGSLITAQQALDLGRTVFSVPGNILTEHSTGCHHLIQDGAVCTVSG
                     EDILAEFKE"
     gene            30449..32527
                     /gene="topA"
                     /locus_tag="IXT20_002932"
     CDS             30449..32527
                     /gene="topA"
                     /locus_tag="IXT20_002932"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002380330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HAQ4861291.1"
                     /translation="MAYKYLVIVESPAKAKTIEKYLGRNYKVMASVGHIRDLPKSKMG
                     IDFENNYEPHYISIRGKGDVIKSLKAAAKKAQKVYLASDPDREGEAIAWHLAYLLGLD
                     LKEKNRVVFNEITKDAVKAAFKEPRTIDVDLVDAQQARRTLDRIVGYSISPILWRKVK
                     KGLSAGRVQSVALKIIIDREKEIREFVPEEYWSIDGNFKKARKKFKANFWGIDGKKKK
                     LPDAQSVKEVTARIDGTDFEVKKVEKKERKRNPANPFTTSSLQQEAARKLNFRTRKTM
                     MVAQQLYEGITLGKQGTVGLITYMRTDSTRIADTAKAEAAAFIEDTYGKEFSRHDHRK
                     IKNAQGAQDAHEAVRPTSVLRTPDELKQYLDKDQLKLYTLIWSRFVASQMTPAILDTM
                     KVTLEQNGVIFIANGSKIKFKGFMQVYVEGRDDGKEEKENILPELEEGDVVQSVDIEP
                     KQHFTQPPARFSEATLIRALEENGVGRPSTYAPTLDTIQRRYYVKLTQKRFEPTELGE
                     IVNSLICEFFPQIVDIHFTAEMEGDLDKIEEGTEAWVKVVDRFYKPFEKELTNAEEKI
                     EKIQIKDEPAGFDCDVCGHPMVIKLGKYGKFYACSNFPDCRNTKPIVKEIGVTCPVCH
                     EGQVIERKSKKNRLFYGCSRFPECEFTSWDKPVGRNCPKCDGYLVEKKVKGGKQVVCV
                     NGDYEEDIQK"
     gene            32496..33872
                     /gene="trmFO"
                     /locus_tag="IXT20_002933"
     CDS             32496..33872
                     /gene="trmFO"
                     /locus_tag="IXT20_002933"
                     /EC_number="2.1.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FADH(2)-oxidizing
                     methylenetetrahydrofolate--tRNA-(uracil(54)-C(5))-
                     methyltransferase TrmFO"
                     /protein_id="HAQ4861292.1"
                     /translation="MEITKKTSKNNRKEVLSLKMVNVVGAGLAGSEAAWQIAQAGVPV
                     RLYEMRPVKSTEAHHTSNFAELVCSNSLRGNSLANAVGLLKEEMRRLNSVVITSADET
                     AVPAGGALAVDRDSFSETITRKVKEHPLVTVINEEITEIPEGITVVATGPLTSHPLAE
                     SIKAFNGSDGFYFYDAAAPIVDKATIDMDKVYLKSRYDKGEAAYLNCPMTEEEFKAFH
                     EALINAEVAELKSFEKEKYFEGCMPIEVMAQRGFKTMLFGPMKPVGLEDPKTGKRPYA
                     VIQLRQDNAAASLYNIVGFQTHLKWGEQKRVFRMIPGLENAEFVRYGVMHRNSFMNSP
                     ELLKPTYQSTKREDLFFAGQMTGVEGYVESAASGLLAGINAARLAKEMEPVEFPQETA
                     IGSMAYYITHAEGKHFQPMNANYGLFPELPERIRDKKARYEAIAQRALKANEAIKTEL
                     EKTAIPNE"
     gene            34070..34975
                     /gene="xerC"
                     /locus_tag="IXT20_002934"
     CDS             34070..34975
                     /gene="xerC"
                     /locus_tag="IXT20_002934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HAQ4861293.1"
                     /translation="MTEATWEQKFLRYLIVERGYSEKTKEAYEEDILHFKHFLEESGD
                     ADLLKAEYFTVRVYLSALADHRPSYSRNSISRKIASLRSFYQYLLKEEVIKENPFSYV
                     HLKKKNLRLPRFFYENEMQALFDSVAGDSPLDLRNRALLEVLYGSGIRLSECSSLSVS
                     DIDFDSEVMLIHGKGNKERYAPLGSFAQDALQEYFREGRQVLMTKYHEEHDIVFVNHH
                     GKPITPTGIEYVLNQMIKKSSLDSDIHPHMLRHTFATHLLNNGADLRTVQELLGHANL
                     STTQIYAHVTKESLQKNYRSFHPRA"
     gene            35012..35560
                     /gene="hslV"
                     /locus_tag="IXT20_002935"
     CDS             35012..35560
                     /gene="hslV"
                     /locus_tag="IXT20_002935"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase proteolytic subunit"
                     /protein_id="HAQ4861294.1"
                     /translation="MVESPFHSTTICAVEKDGKFAMAGDGQVTMGESVVMKGTAKKVR
                     RIYNGEVVVGFAGSVADAFTLEEKFEGKLNEYNGNLQRAAVELAQEWRTQQSMQKLEA
                     MLIVMNDKEMLLVSGTGEVITPDDGILAIGSGGNFALAAARAMKNYAQNDMSAKEIAE
                     NALNIAADICIFTNHNIIVEEL"
     gene            35574..36974
                     /gene="hslU"
                     /locus_tag="IXT20_002936"
     CDS             35574..36974
                     /gene="hslU"
                     /locus_tag="IXT20_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HAQ4861295.1"
                     /translation="MYELKQTPRQIVSELDEYIVGQHAAKKSVAVALRNRYRRLQLDE
                     KMQQDITPKNMLMIGPTGVGKTEIARRLAKIVNAPFVKVEATKFTEVGYVGRDVESMV
                     RDLVENAIQIVEKEQYSRVYTSAKKNAEKRLVKILVPGIKKEQKQSTNQFEQMMNLFN
                     ASQQNTAEPQEEVTEDIRINRRTVLEQLQKGLLDSREVTIEVEDQKKPAPMMNNGLEQ
                     MGIDLNETLGALTPKKKVERTVTVKEALELLIKEESAKLVKDADIHSEAIKLAEESGI
                     IFIDEIDKITSKSQQSGEVSREGVQRDILPIVEGSQVNTKYGIIQTDHVLFIASGAFH
                     LSKPSDLIPELQGRFPIRVELDDLTAEDFVKILTEPNNALVKQYVALIGTENVSVTFT
                     KEAIERIAQIAFDVNRDTDNIGARRLHTILEKLLEDLLFEAPDMQMGEITITEAYVDD
                     KLGSIAANEDLSRYIL"
     gene            36992..37783
                     /gene="codY"
                     /locus_tag="IXT20_002937"
     CDS             36992..37783
                     /gene="codY"
                     /locus_tag="IXT20_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HAQ4861296.1"
                     /translation="MTDLLTKTRKINTLLQQKNTFDMQADLPYDKMAVTLGGILDSNT
                     YIIGSDGVLLGYNERHDVNNERIKTMFVEKQFPESYTDTVAQLMKTEANIPITSDITA
                     FPVESREKYPFGLTTVVPIFGAGERLGTIILSRMEQSFADDDLVLAEYGATVVGMQIL
                     YQKSRSIEADVRSATAVQMAINTLSYSELKAVQAIFEALDGDEGRLTASNIADSIGIT
                     RSVIVNALRKLESAGIIESRSLGMKGTYLKVLNSRFKDELAKHSY"
     gene            37865..38740
                     /locus_tag="IXT20_002938"
     CDS             37865..38740
                     /locus_tag="IXT20_002938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HAQ4861297.1"
                     /translation="MTVIIQNDQLIAEIAEHGAELISLKSKEHGLEYIWQGDPAYWGR
                     HAPVLFPFVGRLKDDQYVYQGKTYQMGQHGFARDMDFEVIEHGQEMASFILRSTPETL
                     EKYPFDFELVISYELGGDGITVHYQVANTGEKEMFFSIGGHPAFNVPLEEGLSFEDFY
                     LAFSPLKSRLRLPLKGPFIDMDQKTLGQTNTNLALNRELFEQDALVFETRGLNAYAIR
                     SEKSSHSVTLSYKDMPYVGIWSPYPKEAPFVCIEPWCGIADTVNSSGNLMEKVGINQL
                     GAHEIFKTKYSITVK"
     gene            complement(38872..39510)
                     /gene="plsY"
                     /locus_tag="IXT20_002939"
     CDS             complement(38872..39510)
                     /gene="plsY"
                     /locus_tag="IXT20_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HAQ4861298.1"
                     /translation="MKLILLLILAYLLGSIPSGVWIGKLFFKKDIRQHGSGNTGTTNT
                     FRVLGKTAGIVVLLMDILKGTLATCLPMIFHLSGINPLWFGVCAILGHTFPIFAGFKG
                     GKAVATSAGMLLGFNPVFFVYSCIIFIVSLFCTSMVSLSSMISAVLITLSTIVLPYVA
                     PVILAKPNWLLTIIAFLVSSFIFYRHKDNIKRIRNHTESRIPFGLNSSKKKS"
     gene            39637..40071
                     /locus_tag="IXT20_002940"
     CDS             39637..40071
                     /locus_tag="IXT20_002940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HAQ4861299.1"
                     /translation="MSVKNPTQETIFNYLEEAKNIAVVGLSNKPERTSYMVAQVLQDN
                     GYKIIPVNPLLAGEEILGEKVYANLTEIDDPIDIVDVFRRSEFLPEIAGDFLKTDAKV
                     FWAQLGIENKEAAEMLRKAGRTDIVMDRCIKIELGKLKENQK"
     gene            40237..42282
                     /gene="parE"
                     /locus_tag="IXT20_002941"
     CDS             40237..42282
                     /gene="parE"
                     /locus_tag="IXT20_002941"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HAQ4861300.1"
                     /translation="MAKKVNNEYNDSSIQVLEGLEAVRKRPGMYIGSTDSRGLHHLVY
                     EIVDNAVDEALSGYGSEIDVTIHEDNSITVADSGRGMPVGMHASGIPTVEVIFTVLHA
                     GGKFGQGGYKTSGGLHGVGASVVNALSKWLIVTIVRDGVEYQQKFKNGGKPDGTLKKI
                     GKTKKANGTTVHFLPDDTIFSTTKFSYEILAERLRESAFLLKGVKISLSDLRGEEPVK
                     EIFHYEEGIKEFVDYLNEEKDTLTPVVYFSGEKEGIEVEVAYQYNDGYSENVLSFVNN
                     VRTKDGGTHEAGMKAAMTKSYNEYARKVGLLKERDKNLEGSDFREGLAAVLSIRVPEN
                     LLQFEGQTKEKLGTPVARTVVDNVISEQMGFYLQENSEMSQMLVRKAIKAREAREAAR
                     KAREESRNGKKRKKGESLLSGKLTPAQSRNPKKNELYLVEGDSAGGSAKQGRDRKFQA
                     ILPLRGKVINTEKAKMQDILKNEEINTMIYTIGAGVGPEFSIEDCNYDKVIIMTDADT
                     DGAHIQVLLLTFFYRYMKPLIEAGKVYIALPPLYKVSKGQGKKQVIEYAWTDDELAAM
                     IKKVGKGYMLQRYKGLGEMNAEQLWETTMDPTSRTLIRVRIDDAAQAERRVTTLMGDK
                     VEPRRKWIENHVQFTLEEDGSILDKKEDTEISPSVSNDLLDEERADKNENNQLFEVE"
     gene            42294..44744
                     /gene="parC"
                     /locus_tag="IXT20_002942"
     CDS             42294..44744
                     /gene="parC"
                     /locus_tag="IXT20_002942"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HAQ4861301.1"
                     /translation="MENRQEVQELTLEEVMGDRFGRYSKYIIQERALPDIRDGLKPVQ
                     RRILFAMNKDGNTYDKGFRKSAKSVGNIMGNYHPHGDISIYEAMVRMSQDWKLREVLI
                     EMHGNNGSMDGDPPAAMRYTEARLSKLSGEMLADIEKETVDLVWNFDDTEKEPTVLPA
                     RYPNLLVNGSTGISAGYATEIPTHNLAEVIDGTIYMIDHPQASLEKLMEYIPGPDFPT
                     GGILQGKDEIKKAYETGRGKVILRSKTKIEAMKGNKQQIVITEIPYEVNKATLVKKMD
                     EIRLNKKIDGIAEVRDESDRTGLQIVVELKKDANAQGILNYLFKNTELQINYNFNMVA
                     IDHMTPHQVGLKDILRSYIEHRKQVITKRSQFDLAKAQKRQHIVEGLMKALSILDEVI
                     ATIRESKDKKDAKKNLVDVFQFTEEQAEAIVTLQLYRLTNTDITELQKESESLIAQIT
                     ELNKILSNDKELFSVMKKELREVKKNYSSARLTTIEDEIEEIKIDTQVLVAQEDVIVS
                     VTREGYVKRTSLRSYSASKPEEIGMREGDYLLYSGELSTLDHVLLITNKANVIYRPVH
                     ELPDLKWKDAGEHISQTISNLSVDESILAVFPYQKIQAEKTFVFISKNGLIKQTRMTE
                     FEPWRTYKSRPLSCMKLKAEEDELIAVYLKEDQPGLDVFLVTHQGMGLRYPLTDVPVV
                     GAKAAGVKSMNLKDGDRIINGLLVLAEGDTPIVIVTQRGAVKRMLAQEISQTSRAKRG
                     VTVMRELKKNPHRIIYMSEGRSKEITLMNQKGQQLTIDPTDFPIGDRTSNGSFAMDEK
                     KGGEVIEVIDAPEIFIES"
     gene            45021..47252
                     /gene="pflB"
                     /locus_tag="IXT20_002943"
     CDS             45021..47252
                     /gene="pflB"
                     /locus_tag="IXT20_002943"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HAQ4861302.1"
                     /translation="MEQWNGFKGTKWKEGVDTRDFIQNNYTEYRGDDSFLEGPTEATE
                     KLWDELQKLNDIQFERNGVYDMDTEIVSTITSHDAGYLDKDLEKIVGLQTDKPLKQAF
                     MPFGGINMANNALKSNGYTPNDELTHIFTDWRKTHNQGVFDAYTAEMRAARKNKIITG
                     LPDAYGRGRIIGDYRRVALYGIDYLMEQKKKDHDMTGYHTMSEEVIRLREEITEQYRA
                     LAQMKEMAAKYGYDISKPAANAKEAVQWLYFGYLAAIKSQNGAAMSIGRISAFLDIYI
                     QRDLDNGVITEKEAQELIDHLVMKLRMVKFARTPEYNQLFSGFPIWATLSIAGMGLDG
                     RSLVTKNDFRLLHTLTNMGPSPEPNLTVLYSEHLPEGFRTYASRIAIESSSIQFENDN
                     QLREQWGSDDCAIACCVSGTVVGKDMQFFGARANLAKTLLYAINGGVDEVTGAQVGPE
                     YRPITSEILDFEEVKHNFMNMMDWLAELYVNTLNIIHYMHDKYSYEAAQLALMDSDLQ
                     RTFATGIAGISHAADSLSAIKYAKVKAIRNEKGIAVDFEVDGDFPKYGNDDDRADELV
                     SWILEYFMGQIKRHKTYRNSKPTTSLLTITSNVVYGKNTGNTPDGREAGKPLAPGANP
                     SYNAEQSGLLASLNSTAKMNYGCALDGISNTQTINPSGLGKEQDTRIENLRNVLDGYF
                     SKGAYHLNVNVFSNDLLRDAMENPMKYPNLTIRVSGYAVKFRDLTREQQLDVLMRTSH
                     DRM"
     gene            47496..48257
                     /gene="pflA"
                     /locus_tag="IXT20_002944"
     CDS             47496..48257
                     /gene="pflA"
                     /locus_tag="IXT20_002944"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase-activating protein"
                     /protein_id="HAQ4861303.1"
                     /translation="MEEKTIGYVHSIETFGSVDGPGLRFVVFMQGCRMRCQFCHNPDT
                     WNIGGGKEYTADELLDKAERFRPYWGDKGGITVSGGEPLLQIDFLIELFKKAKERKMH
                     TTLDTCGKPFTYEEPFFSRFQELMKYTDLLLFDIKHIDNEEHKKLTHWDNENILEMAQ
                     YLSKINKPVWIRHVLVPERSDYDEYLIRLDNFIQTLSNVDRVEILPYHTMGKYKWETL
                     GLKYPLEGIEPPTKERVENAKRLLHTEEYTKYLTR"
     gene            48342..49268
                     /locus_tag="IXT20_002945"
     CDS             48342..49268
                     /locus_tag="IXT20_002945"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-dependent inorganic pyrophosphatase"
                     /protein_id="HAQ4861304.1"
                     /translation="MSKILVFGHQNPDTDAIGAAIAFSHLQNELGKETEAVALGTPNE
                     ETQYALDHFQLSAPRVVESAKAENADVMLVDHNEFQQSISDISEVNILAVVDHHRIAN
                     FETANPLYYRAEPVGCTSTIILKLYKENQVTIPKEIAGIMLSAIISDTLLFKSPTCTP
                     EDEAAAKELAAIAEVDLNTYGLEMLKAGTNLSDKSAAVLLDLDAKSFPMGSSNVRIAQ
                     VNTVDLNEVMDRQAELEAAMEDEMKANGYDLFVLVVTNILDSDSELLVAGKGKEKVEE
                     AFQTTLTNNRALLKGVVSRKKQVVPQLTAVFS"
BASE COUNT        17101 a         8484 c        10459 g        13346 t
ORIGIN      
        1 tcaattctga ttcatcagtc ccataaatta tagagccaat aaaaataaac atacaaaaag
       61 ggcggatatt caaatggata ttccgtcttt ttttatttga tgctggaaag tttgcatttc
      121 tctcttattt ttttaaggta taatcatact tgattaagaa aaaggggtca ctcagataga
      181 aaaggtggga gaaagtgaaa atatttagac gaatactaat tggcttagtc atcattttag
      241 gattactcgg tggagccggt tattactata tacagcaaaa tacgtacgaa ccatcgtcag
      301 aagcgcaagt gttggtaaaa gaaggaaaaa acaaagaaga ttacttattt tttgaaggag
      361 agaaaaagaa tcatggaaag tctccattac ttatttttta tcctggtgct ctcgtagagc
      421 cagaaagtta tagtatttgg gcgcaagatt tagcaaaaaa cggcttttca gtcgctattg
      481 tcaaaatgcc acttgatcta gcagtgatgg gcggaaacaa agcggataag gtcaaagaag
      541 attttccaaa ttccgattat gtgatcggtg gccattcatt aggtggcgta atggcaagtc
      601 gttatgtcaa taagaatcat tcagacaaac atttaaaagg ggtattcttc atggcaagtt
      661 atcccgacaa aaaaggaaca ttgacagaat cagctgttcc tgttttatcg atcacggcga
      721 caaaagataa agtattgaat caggaagctt atcaagaagc taagcagtat ttaccggaaa
      781 gaacagagta tgtatcaatc aatggcggaa atcatggagg ttttggcagc tatggtgagc
      841 aaaaaggaga tggagaggca gaaatttcta acgcagacca gcaaactgtg atcgtcaata
      901 cattggaaaa ctggctgaaa aagttgaaat aatataaatg ggtcctatag ggtccgtgaa
      961 aaaactagtc aattaactat agatagcaaa tgaatctttc aaaataagtc gggatttcac
     1021 ggaacaagag aaacagatcc cggcttattt tcaagtttag agaacttagt gtacgttttt
     1081 aaagtttcgt ttatatttat attttatttt ctaataaaat cgctcgaact ggacattttt
     1141 gatacgcttt ttttacatat gtttcttctt ccggcatcac gaactgttga tgagcctctg
     1201 gttcttgggc aaataaaacg attccttctt catcataatc gaaaatatct ggcgcttcga
     1261 tttgacaaag accgcatgca atacagcgtt caggtacaag tttacatagt agtgacattt
     1321 ctaaccatcc taacttaagg agaataatat gattgttttt attttatcgc tattttcatc
     1381 aggacacaag ctgcggatta gttctcttta ccaactcctt gtgggaaaac gaacgacttc
     1441 agtacttatt tatggattta cccacgaact tttgtttatc cataacagtt ttccagaatt
     1501 gaagcaggat aagttttatc aaattttgca aaaaattgcg cagcaaggtt ggatcgaaat
     1561 aaatgaaaat gaagcaaagc taacgcctgc aggggctgat atgttgtccg aacatcaaat
     1621 agaacatact ggtttgcgtt ttgaccgcta tggcagaaca ggggaaacta gttggcggct
     1681 cattaaattt gctgttcaag taatttctaa tctggcaacg ggtattcaag actatcttcc
     1741 agcagagaca agtccttttt atactttcca actaaaaaaa tggcttagtg aaagccaatt
     1801 gccacgtgaa attttgattg atactgcgta tgagagtttg gtgcagctgt tttcggagat
     1861 accagaagaa gcagcagatt ttttagctaa ccaattatct ggaaatgagc gaacagggct
     1921 gcttccttat cagctggcaa aaaacaatga tgagtcagcg gtttatctgc aacaaagcag
     1981 atgtatccac cttttgttag cacaaatcga aaaacggcca gattcgcttt ggcacgcttt
     2041 gatcgattca ttattacagc aaaatttcaa tcaaagcatg atgatcacca agcagatgtt
     2101 tatgaatggt cagacaattg accagatcat ggcaatccgt catttaaaaa gaggaacagt
     2161 aacagatcat cttattgagt gggccttgtt ttttgatgat tttccatacg agtggattct
     2221 ctctgcagaa acaatcgaac gtttggaacc aaataaggat tccgtgcgag agtggcgctt
     2281 tagcgaatgg aatgtagatg gacaattaga ttatggagaa tttcgtttgt atcaaatcta
     2341 tctattgaga aaggaagcga tacagaatgt taataaatga attaaaaaaa tattttggat
     2401 ttgattcatt tcgtcccggc caagaagaaa ttattcaggc gttgcttgat ggagaagata
     2461 ctctcgctat tttgcctaca ggaacgggaa aaagcctttg ctatcaattg acggggtatt
     2521 taatggaagg tctggtcatc atcgtgtcac cgctgctttc tctaatggaa gatcaagtga
     2581 cgcaattaca aaaacgaggg gagaaacgag tagctgcttt caatagttta ctttcgcaat
     2641 ctgaaagaag atacgtcctc cgtcatctat cgcaatataa atttttgttt ttgagtcccg
     2701 aaatgttgac taatccttct cttttagagc atctgaaaaa acaaaatatt gcgttatatg
     2761 tagtagatga ggctcattgc gtgtctcaat ggggagttga tttccgtcca gaatatcaac
     2821 agttaggaaa aatccgaaag cttttaggaa acccggtcac actggcacta acggcgacag
     2881 caacagattt agtagcaaga gatatacgtc atgtactatt tgatcgacca ccaaaagaga
     2941 tcagacaatc agtcaatcgg caaaatatct cgctttttgt tcggaaaacg cagcaaaaag
     3001 aacaagaact tgaacacttc atggaaagag cacaaggagc ggcaatcatt tattgtgcaa
     3061 caaaaaaaga agtcgaacga ttgtatcatt tattcaggga acgttttaca gttggatact
     3121 atcacggtgg tcttgatgca gctcaaagaa gacaacttca acagcagttt gtaaaaaaac
     3181 agctgcagtt tctcattgct acgaatgcat ttggtatggg gatcgataaa tctgatatcc
     3241 gttatgtggt ccattatgat ttgccagaca gtctagaaaa ctatgtacaa gaaatcggtc
     3301 gagcaggcag agatcaggaa gaaagtgcag caattctttt ttatcaaagt ggggatgaac
     3361 gtatccatta tttctttaat caactttcta gagaacagcg acaaagtttt gagctttatt
     3421 tggaatatgc cacagagcaa gctccctttg atgagctgca aaaaaaatgg atggagctga
     3481 tccagcagtc tgaaaaacca gaaaattggg tggaacggct caaacgtcag gaaaaagaaa
     3541 aagaattccg cctgcagcag atgcttcgct atattaatga agaaaactgc cgcagaaaat
     3601 tcatcttagc ttattttggc gaaaaactct ccgaaaagcc gcagaattgt tgtgatattg
     3661 atggagcaaa agcagaactt ctgccaaaga ggaagctttt ttctaagact gaacaacttc
     3721 attgggaaac gattttacta aatttattta aaaaaaacat ggaacattga ttttttagga
     3781 caagacactt tttcctatgg tataatacac acgatagagt ttaggaggta aaacagtgag
     3841 cagaaaagat agacatcaat ctacagaaaa taacgaaaca caagaaccat gggaacaacc
     3901 aatctacgat acagatgatg aagtttcatc aagaagttct cagagacaac aaaagaaagg
     3961 aaatacatta tttctttcgc tatttttgat tttactggtc ttatgtatcg ctattccagc
     4021 aggtgcttat ttctggatca gaaatggatc aagcaacaat gcaacagcag caacttcttc
     4081 tgaaacttcg acatcagtag tagaaagttc tacaagtaca acaaaagagg agtcaactga
     4141 gacttcaaca gtagaatcta gtacaatcgc ggaagaatct actgtgtctc aagatacagg
     4201 tgtaacgtca gaaagcagta ttgcagcaga gacaacaccg acttcctcaa gtgaggcaac
     4261 ttatcaagca ggggattcaa gcagcaatgc agcagaaaca acaccttctt catcagctgc
     4321 agccggttca acaacgactg ttcaagcagg cgaaggtcca aaacaagtgg ctgagcgtgc
     4381 agggatcacc actgatcaat tgtttgaatt gaatggctta gatccaaaca actttatgct
     4441 ttaccctggt caagaattaa gaattaaata ataatagccg cgctttgcgg ctttttttct
     4501 gagaaaaagg agttatatca tgacacaaat cagtattgca atcgacgggc ctgcttcatc
     4561 tgggaaaagt accgttgcca agatattagc aaaagaattg aattatattt atacagatac
     4621 cggtgccatg tatcgcgcag taacttattt agctattacg aacaatgtat ctttttcaga
     4681 tgaagaaggg ctagtttcgc tgatcaaaaa gtatcctatc tctttttcgc aacaagaaga
     4741 tggacagcat gtattcatag catcaaaaga tgtgacttta gaaatccgtc aaccagatgt
     4801 cacaaaagct gtttctgaag tctctgcaca tggaaaagtc agagaagaat tagttgctgt
     4861 ccaacgaaaa ataggggaac gcggtggcgt tgtcatggac ggaagagaca tcgggacagc
     4921 tgtattaccg aatgcagaag tgaaaatctt tttagtagca agcgtaagtg agcgtgcaga
     4981 aagacgtttc aaagagaacc aagaaaaagg aattccgact gatcttgaaa cactgacgaa
     5041 agagatacag gaaagagacc attatgattc aacaagggac gtctctccgc tgaagcaagc
     5101 agaggatgct gttcgtatcg atacgactgg caaatcgatt ccagaagtcg ttgcagcaat
     5161 aaaagctgtt gttttgaaaa aaggttatct tcttttttaa attaagaaaa aaaatgaaaa
     5221 acaattgttt tttcaaggaa aacgaacgaa atcgctttat tttttttgaa taatcaatta
     5281 aactaaaaac agtaccgtac ttacggcaga atgttggtta ggaggacaag tgttatgaca
     5341 gaatttgatc aaaatcaaga gaacaaccag tcaatggaag acgcaatgaa aagcgtacaa
     5401 gaagtaaaag ttggcgatgt tgttaagggc gaagttttag tcattgaaga caaacaagtg
     5461 attgtcggaa tcgaaggggc tggagttgaa ggagtcgttc cggcaaaaga attatcaact
     5521 ttgccggtag aagatatcag cgaagtagta aaagtcggtg atgtattaga tttagtcgtt
     5581 atcagtacga tcggcaaaga taaagaaaac ggcagctatc tactttcaaa acgccgttta
     5641 gatgctaaaa aagtttggga agatatcgaa agagatttcc aagagggcaa ggtcatcgaa
     5701 gcacctgtta caaatgttgt caaaggcggt ttagtcgttg acgtcggtgt acgtggtttt
     5761 gtccctgcat caatggtgga agatcatttt gttgcagact tttcagacta taaaggcaaa
     5821 acattgagct ttaaaatcat cgaaatcgaa ccatcagaaa atcgtttgat cttatcgcat
     5881 aaagcggtag tggaaaacga aagagaagaa aagaaaaaag aaattctttc aactatccat
     5941 gagggagatg ttctcagtgg acgtgttgca cgtcttactg attttggcgc atttgtcgac
     6001 ctaggcggaa ttgacggtct agtccatgtc tcagagattt cacatagtca tgtagcaaaa
     6061 ccaagtgatg tattggctgt caatgatgat gtaaatgtca aagtgttgtc tatcaatcct
     6121 gaacaagaac gtgtttcctt gtcaatcaaa gacacacagc ctggaccttg gtcagaaatc
     6181 gatacaaaag ctccagttgg cacagtatta gacggaacag ttaaacgttt gacaagcttc
     6241 ggtgcctttg ttgaagtgtt ccctggagta gaagggctag tccatatctc tcaaatttca
     6301 cataaacata ttgcgacacc acatgaagtg cttcatgaag gtgacgaagt gaaagtgaaa
     6361 gtattagaga tccatccaga agaacatcgt attgcattga gtatcaaagc tttagaagaa
     6421 aaaccagctt cagaagaaga accaaaaaat gttgagtctt atgaattacc agaagaaaat
     6481 actggattta ctatgggaga cattttaggt gatgcgttga aagaagaagt agaagaaaaa
     6541 gcaaccgatg attctgaaaa ataattgatt ttgtatctgt gactgagatg aaagcagctg
     6601 cttttgtctc agtttttctt ttgaaaagcg attagtattt caataaggaa taaaaatact
     6661 tcgtttaatt gacaatctat gcttgaaagt tggcaatgca ttcgttaaac taagtgagat
     6721 tggaaacttg tgaggaggaa ataatatggc aaatccaaca attgcaatcg ttggccgccc
     6781 gaacgtcggt aagtctacga tttttaaccg catcgctggt gagcgtatct cgattgtcga
     6841 agatactcct ggagtaactc gtgatcgtat ttatgcaaaa ggtgaatggt taggtcgaga
     6901 attcagcgtg atcgacacag gcggaattga tcttggcgat gaacctttta tggatcagat
     6961 caagcatcaa gcagaaatcg cgattgaaga agcagatgta atcatttgtg tagtcagcgg
     7021 acgcgaaggc gtgacagacg cagatgagat ggttgcgaaa attttataca gaagtaataa
     7081 accagtgatt ttagcagtca ataaagtaga taatccagaa atgagaaacg atatctatga
     7141 attttattcc ttagggctag gcgatcctta tcctgtttca ggaagccatg gactagggat
     7201 tggtgatatt ttagatgaag cagtgaagta tttctctgaa gagactgaag aagaggacga
     7261 cgacacgatt aagtttagtt tgattggtcg cccaaatgtt ggtaaatcct ctttgatcaa
     7321 tgcgatttta ggagaagaac gtgtaattgt ttcggatatt gaaggaacga cccgagacgc
     7381 aatcgacacc cacttcgttt ctgaaagtgg tcagaaattc ttaatgatag atacagctgg
     7441 tatgcgtaaa cgcggaaaag tatacgaaaa tactgaaaaa tacagcgtga tgcgtgcaat
     7501 gcgtgccatt gaccgttctg atatcgtttt gatggtactg aatgctgaag aagggattcg
     7561 ggaacaagac aagcgagtag cgggttacgc ccatgaagct ggtcgtggaa ttattatcgt
     7621 cgtgaataaa tgggatcttg taaagaaaga aaccaataca atgcgtgatt tcgaacaaga
     7681 gattcgtgat gaattccggt atcttgatta cgctccaatc gtttttgttt ctgcagtgac
     7741 taaacaacgg ttggaacgtc tgcctgaaat gatcgaacaa gtaagtatga accagaatct
     7801 tcgtatctct tcggctgtgt tgaatgatat cattatggat gctgtagcaa tcaatccaac
     7861 accaacagat aaaggaaaac gcctgaagat tttttatgct acacaagtcg ctgtcaaacc
     7921 acctacattt gtcgtttttg tcaatgaaga ggaattgatg catttttctt atgaacgttt
     7981 cttagaaaat caaatcagaa aagcatttac ttttgaaggg acgtccatcc gaattatccc
     8041 tcgtaggaga aaataggcat tttaccatat cgacgttttt ttttcaaaag aaaaagacag
     8101 tttcgtttgt tacgtttcta tgactaaaaa aagcatgtga aaattggtaa aaaccttgct
     8161 attaagcggt tcatatgata tcgtgataac agaatattga agttaatcaa tatttctttt
     8221 taaacttctg gaccactcag aaagttttaa ctaaatgata tttctatcat atctcttgtg
     8281 gaggaggtga atctatccat ggcaaacaaa gcagaattaa tcgaaaaagt tgcttcagct
     8341 actgatttaa ctaaaaaaga tgcaactgca gcagtagatg ctgtattttc aactattcaa
     8401 gatgctttag ctaacggcga aaaagttcaa ttaatcggtt ttggtaactt tgaagttcgc
     8461 gaacgcgcag ctcgcaaagg tcgtaaccca caaactggtg aagaaatcga aattccagct
     8521 agcaaagtac ctgcattcaa accaggtaaa gcattaaaag acgctgttaa ataagacagt
     8581 tgaaaaagcg cttgtggctc tagggctaca agtgcttttc tttttatttc ctagcagaaa
     8641 aggaataaat aagagacaga gattccgaat ccgtatgtca tacatagaaa acacatgtat
     8701 tttatttaat ttttatttat ttgcaagtta tgaggtgagc tttgctctta tcttttctag
     8761 aatttgctta ctaatggtaa aataaaaata agaagaggag ggatgcgtat ggaacttgat
     8821 tatcaagtag aagatgcgtt gacgaaagtg ttttacggag aaacgctagt cagtcagcta
     8881 agagaacttt ccattgatca gccaatctta tttttaacga atcagcgcta ttatgatttg
     8941 tttgcagata agatcaaccc gttatttgca aatcaagcaa acaatgactg gtatatttgt
     9001 gccaacactc aatgcaatca tttaacagaa ttaaagaacc tgcttgattt cgtcaaacgc
     9061 tatcccgaaa atcaatccat gtttatcatc ggatttggaa atgaagggat catggatctg
     9121 gcaggttttt ttcaaaaaca tacaaccctt gattcatcac tatggatgat ccctgtatcg
     9181 atccgttctc taagccgagc attaacaccg cagcggacga ttttgcagct accaaatcgt
     9241 gtagtgctgc aaaccatcaa tctgccagaa cgcatcgttt atgatcaaac aatcagcgag
     9301 cggcaggtgg acggaaagca gattgacttt ttgactttta tctgttgcgg aatgatttgt
     9361 gatcatcgtt ttttgcaaaa tctgtataaa aactatccaa ataaaaagca attaacgact
     9421 cggccgtttg ccgggatgat cgaagaaatg atctattttt atcaattaga ggcagaaaac
     9481 ctgtcaaatt atggtaaact gtttgaacaa gctttttatc tgaccgaaaa tggccatcta
     9541 ctttctgcta gtatgaaaaa atttttagga atgatgtttc atttcgtgtg gaatctccaa
     9601 gcatatcctt ttgatttcca actaaaaaac ttttttgtgt ggctggactt gcttggctat
     9661 ccaatccact ggctggaaca gatttcaaag atggagtatt tggaaaaagt tctttttttg
     9721 gcagaaaaaa gcaaaaaaat acttgtttta gaaaaaatag gtataataga cggataccaa
     9781 catccaaacg aaaaagaatt attgagaaca atgacggtat ttcaacaaat cgtcagtgag
     9841 ataagaggga attgaaatga atacaaatag cgaaaaaatg ttgcaagctt tatcagatga
     9901 agacttgtca caagcacaaa tctttttaga aaaagcatta aaagaagacc cagcagatat
     9961 attagctgaa ctaggcgaag aattgctggc tattggcttt ttgatcgaag caaaacagat
    10021 ttttgaacaa ctgatcaacc aatacccaga aaatgacgga ctgaacatcc cgttagcaga
    10081 aattgcaatc gaagataatg agatcgactt agcttttgaa tacctggaaa aagtggataa
    10141 gcaaagtgat tactatccgc aaagcttatt ggcgatagct gatttgtacc aagttatcgg
    10201 aatccctgaa gtgagtgaag caaaattgaa agaagcagct gggttgcttc ctgatgaacc
    10261 attgatccag tttgcgctag ctgaattgta tttttcagtt gatcgtttca aagaggcagc
    10321 agtggtttat gaaacattgt tggcaagcgg gataaatgaa atttcaggaa tctcgatgca
    10381 ggaacgttta ggacaatcct taagtatgca aggagaattc gaagcagcta tcgtaccttt
    10441 agaagaagca ctgaaagaag aaaggacaga tgatcgactg ttccagctag cttttacgaa
    10501 tcttcaattg aaagaaaatc agcaggcaat caactatctg gaagaacttc gagaagttaa
    10561 tccgcattat caatcgttat atctatatct aggacaagct cttcaagaag aagaaatgat
    10621 tgaagaagca caaacagtgt tagaagaagg aatcaaagaa aatccttacc aagtcgaact
    10681 ttatcacctg gcctcggaaa atgctttccg tctgcatgat agacagcgag cagaagaact
    10741 tttactgaaa gctttagaag taggagacaa acaagatgaa acattactta ctttaagtaa
    10801 tctttacttg gatgatgaaa gatatgaaga tgtgatacaa gtgatcaatc agatggaaga
    10861 aactgctaat ccttatgcag aatggaacct agcccatgct tataatgaat tagaagactt
    10921 taccgttgca gctgtacact atgaacaggc ttatcatgaa ctatcacatg aaccagattt
    10981 cttgaaagag tatgcctttt tcttaagaga agaagggcag ctgaaaagag cgcaagaatt
    11041 attgacttat tatttaacac ttgaaccagg agatatggag gcactgtcat tattagatga
    11101 cctaacagag aggtgagaaa aatgctaatt gatgtacggg aaaagaagaa ttttttaacg
    11161 tggatggtca atcatgtatc ttttagccga agagaagttt tttggatctt gaattatctt
    11221 gccaatcatg aagcgatttt aagcaatgtc cactttgttg aaggggcgaa ttttgcggat
    11281 cgaggactgc agatcagtga tctatctgtt gaaggggaac ccatgaaatt gtttttgcag
    11341 ggaaaagaat ttacggacac agaccagatt tttcatgaaa tccgtttgaa ttggaaaaag
    11401 cctttatatg tggaatgtat attcgaaaat gcttggcaga caagagaata tctatctatc
    11461 ttagaagaca atccattttc ttcttgggat tcttcagtca gtgaagaaga tactgaatta
    11521 attgaacgat attttcaaga aaaagaagaa gaggcaaaac taaaaatgct ttatgctcaa
    11581 atcgatcaag cactagaaga tggagatagg gatgcttttt tgaaattatc tgatgaagtt
    11641 aaccgaaaga tccttttgca gacacaaaag aaaagtaacc gggtaagtga acataacaat
    11701 tagttaaaaa tagtatagta ctaaaaaaga caaaggtcag ctggatgtgg aatctttcct
    11761 catagcctat ctttgtcttt tttcttatag ttttttcagt aatcttgtct gtggcttcaa
    11821 ataagtaaag ccttgccctt ccacttcatg cacattgatg attgaaagga aagctttttc
    11881 gtcaatctcg tggacgatcc tcttagcttc agttaattca ctagagctaa cgaccatata
    11941 gatgattttt tttgaattac cagagaatgc gccttctcca gaaaggaaag tcacacctct
    12001 ttgcagaccg gtcatcagta gtggtgcaat ctcttcgctt ttgttggaga cgacaagtag
    12061 accttttgca gaatatccgc catctaagat cgaatcaatc acacgactga aaacatacga
    12121 aacaatcaac gtatacatca tacgtttcaa gtcgatatag gttaatgaag caagtaagac
    12181 gatgatatca aatgccaata gggaacgccc catagtgatg ccgtaatttt gttctaaaat
    12241 ccgagcgata atatcactgc cgcctgttgt tccacccatg cggtagacga tcccgcttcc
    12301 tacacctgca actaaacccg caagtagaga aacgatcagt agatcatgct gtaaattgat
    12361 ttctagtgga accctttgcc agatccataa aaagaatgac aaagaaagtg tacctagaac
    12421 agtataggcc aaggaacgtt taccgaggat ctttcctcct agtaaaatca atgggatgtt
    12481 gattaataaa gtcgtataag ctggatcaat accaaataaa gctcttaaga tcaaggtgat
    12541 ccctgagact cccccttctg ctaaatgatt agcgatgttc aagtaaacaa gaccaaatgc
    12601 ataaatactt gttcctataa tgataaaaag aacttctttt actgtttttc gtttgaccat
    12661 atgaattcca gctttcaatt atgtattttc acagaatttc catttagaaa ttccttccta
    12721 aatgtagcat ggaaattcga ttttctcaat tgctcttatc taggatttgc gttaaagttt
    12781 tggtaagata gttttggagg gaaaaagatg gataaaaagc gttctttata cagtatgcag
    12841 caggaagtgg atgaatatat ccagcaattc aaggtggggt atttctctcc attagctcag
    12901 atggctcgat taactgaaga agtaggtgaa ttggcaagag aagtcaacca cacttatgga
    12961 gaaaaaagca aaaaggcttc tgaaccagtt aactcggtag cagaagaact tggtgatgtt
    13021 ttattcgtta cgatgatcat ggctaattca ttgaatatcg acttaactga agtttttgaa
    13081 aaaaacatgg aaaaattcaa tcaaagagac cataatcgct ttgagcgtaa ggacgagtaa
    13141 acaagatgaa gtggtcctgg ccttgaagac tctgaaacgg acttgtcaaa cagtcaccat
    13201 gcaactgagt ctttatctca ggacaagaaa cacgcaggaa aggttggaaa aatgaaatta
    13261 aaacaattac ctctggaata ccaaaaggca attccagttc tgaaaaaatt agagaatgca
    13321 ggatatgaag cttattttgt aggtggaagt gttcgtgata tcttgctgaa ccagccaatc
    13381 catgatgtgg atatcgcaac aagcgcattt ccagaagaaa tcaaacagct ttttccaaaa
    13441 acgatagata ttgggatcga acatggaact gtgttggtat tagaaaatga tgaacaatat
    13501 gagatcacca catttcggac ggaatcaact tatcaggact accgtagacc agatcatgtc
    13561 gaattcgttc gttcgttggc agaagatttg aaaagaagag attttacgat caatgcgttt
    13621 gctttgaaag aagatggaga aatcatcgat ttgttcgatg gtctgcaaga tctagaagaa
    13681 cagatcttgc gagctgtagg caatcctcat gagcggttcc atgaagatgc cttacgaatg
    13741 atgcgcgggt tgcgttttgt cagtcaacta gggttcaaat tagaggaaga aaccttctac
    13801 gccatcaaag aaaaccacga gctattaggt aaaatatctg tagaacgtat aaatgtggaa
    13861 ttcatcaaga tgctcttggg aaatgaacgg gccgcgggaa tccatgcatt tattgaaaca
    13921 gagtgctaca tttgctgtcc cggtttgaaa gaagaaggcg aagcactttt acgttttgct
    13981 gaattaccat ccgtcccatt gaaagaagag agccaagcat gggcattatt gatcagccaa
    14041 ctggatctaa aagaaaatga gattcgttcc tttttgaaat cgtggaagtg ttctaaccaa
    14101 atgattaaag acgttcaagc acttgtccaa ggactacgac aacgagaaac tggagctttg
    14161 gaaccattcg aattgtactt cttaggcaaa aaacatgcac tgcaagtaga agaattgatg
    14221 atctattttg acgaaacacc tcagatggat gccgtaggag aatcctatga tcgtttacct
    14281 atcaagtcat taagcgaatt agcagtagat ggtaaaatct tgctagaaga aactggaaaa
    14341 aaacctggta agtgggttag tgaagctttg caattagctg aaaaagccgt cgtagaacaa
    14401 agaataaaaa atgataaaaa acaggttttg gattttttat cgaaagaaaa attgatttaa
    14461 aaaacactag actgtatctg ctcagctttg ttggctactt aaagtgggca gatacgtcat
    14521 tagaagaaaa aacataagat gtgaccactt acataaattt gtgctaaact gaggtagtac
    14581 aagaataaat tacatcataa aggagtcttt ttaatgagta aagaaatgac agcgctaaaa
    14641 ttttatttta gaaatggaga aacctggacg atcaaccgcc gtcatatcgg cgatctttgg
    14701 atcaaacaaa tcacaacaag ttttggacga atcaatggca gtgaatttgt tgaaatccat
    14761 ccgtgtgctg gatttaaaat cgaaatcttc catgaagggg acgcagttgc gacacatgat
    14821 atcaacctag gcggactgga aatgggcatg tttaatcgcg cattgaaata cgaagatatc
    14881 gagcgaatgg aaatccttta ccgaaatggc acacctgatc ttgtgtactt cccatatcta
    14941 gataaaggaa cagaaggatt agacaatcaa tatcaatcaa caaaaatcag cgaaaaaaca
    15001 ggtaacttgt atatcgtgat caatccagat caacgtgtag aagatgtata cggggaattt
    15061 ttcgaataaa tcgattttta cgcaaaaatg aggctgtgaa agagatgtgt cttattacta
    15121 ctgataggct gtctcttcca caccgtttat tcagaataag ggactgtgac aagatttttg
    15181 tcgcagcccc atttttttgt tgtgataaaa gaacaagaaa ggcaggaatg ggaaagtaaa
    15241 catccttttg tcagaaactt ttatctttct taagttttac ttgatatctg atactatttg
    15301 gaatagaatg aggcgttgaa aaatttatgg aaatttctaa ctagcaataa acgatgaaac
    15361 aatgaaatat ttttcatcca aaaagaactt tgctataatg atggaagttt gtgtatagga
    15421 gatctggaaa atgagagaga gttttgaaca acaaaaaaaa ctgttgcatg atcgctatgg
    15481 cgccctttcg atggatgacc gccgtcaaat actttgtaag ttgcgtaaaa gaaatatatt
    15541 gatgtatcgt cagttggaac gtctgaaaca tgatctgctt cgtttggagt caaaacgtgt
    15601 acagtgtgaa ttagagggga accaaacaca ggttgaagta gtcgaaacca aaattttaaa
    15661 gaaaaaagaa caatttttaa aaatgcttac acaaaataaa aaataaaggg gatggacgat
    15721 ggaatttgct gagttagtta ttgtatttgc agtaacgatc accttatcga acatagctag
    15781 ccgcattttg ccaatgattc cagctccact gatccaaatt ttccttggag tggtattagg
    15841 tctgacagag tggggacaat caatcgattt tgaacctgaa ttgttcttag ttatgattat
    15901 tgcaccattg ttatttagag aaggagaaaa agcagatatt tcttcgattc tcaaaaattt
    15961 cgggacaatt ttgttcttgg catttggagg agtcattttg acccttgtcg gggtaggtgc
    16021 gacgttatca ttcctgttgc caagtgtccc acttgctgcc tgctttgctt ttggagcagc
    16081 gttaggtccg acagatgcgg tagcagtcag ttctctttct ggacgagtga atatccctaa
    16141 aaaggcgatg catattttgg aaggagaagg tcttttgaat gatgcatctg gtgttactgc
    16201 cttccagttt gctcttggtg cgctgatcac cggatctttt tcagcagtaa acgcggggat
    16261 gagtctagta gtttctagta ttgggggagc tttgatcggc tttttgcttg tctggttcaa
    16321 acaaaaaata attcatttga tcgaaaaagc atcagcacaa gatgtcacag ggtatctgct
    16381 gatcgaactt ttgcttcctt ttctagccta tgtattggca gaggcatttg atgtatctgg
    16441 aatcattgct gcagtggcag caggtattct ccaagcttct ggttttagga aaatctctgt
    16501 gtttgatgca gaattgtcga gcttgtccca cagtacatgg acaactattg cttttacact
    16561 gaatgcatta gtatttatat ttttaggaat cgaattgacg caagtgttct cgccagtatg
    16621 gggagacggt ttatatccaa acggtctttt attagcgatt atcgtattaa tctctgtcat
    16681 gctttttgtg atcagattca tttctatcag tcttttttat gtattcaaag atggcagtaa
    16741 aaagttcaaa aaacagttga acgagatctt gatcttaaca tttggcggtg taaagggaac
    16801 agtcagtttg gctacgattt ttattttgcc attttcaatc aataatatga tgttttacca
    16861 gcggtctttg cttttgtttc tgacggcggg tgtcatctta gtgactttgg tcatagggat
    16921 cattgtcttg cctatgctga cggaaacaga ggaagcgaaa agcacggatt tgaatgcttt
    16981 gatgatttta gaagaggtcg ttgaagtttt gcgaaaagaa ataaaggaaa ttgatcgtaa
    17041 tacaaaagaa tttttagcca ctgaagcagt gatagaaaat tatcaagaac ggatacgtga
    17101 tctgtacttg gaagatttaa ccgatgatga aaagcaagaa gtacaagaaa tacaagcact
    17161 gattttgtcg atcgaacggg atggattgga tgaaagttat cgttcgggca agctttcttc
    17221 taatggttat cgtttttact cccgttttct ttctcgattc gaacattcta tcacaagcca
    17281 gattttgtct tttatcggct tttggttcat tgttgtccgt aggttaatgc gaatcgttct
    17341 ccatccgaaa atgttttggc aaagaagata tgctgatcgt cagacattta ttagtgatga
    17401 agatatccaa gaaatcagag agacatatca aaaaaatact cagttgatta ttgaaagttt
    17461 agataacttg actgatattt atgatgaaac gatggttcaa ttttttatcc agcaacgcaa
    17521 acttgaagga ataaaaatga actcggggaa tttgatttct tcgtggatga tccagcaaga
    17581 tagtctgttt acgaaaaaaa tgctaagagg ttattatctt gaaagaaaaa tcattgatga
    17641 atacgaagtt gctgaaaaaa tcacgacgtt ttctgcgaac agctatcgca ggaatatcaa
    17701 tttgttagaa tcttatacga tgaacaaacc atctgataac ttttctttcc gatttgcgtt
    17761 tcgagcaaag tcaaaaaaaa cgagtccaat aaaataaaaa ggtaaaaatt ttcaagtttt
    17821 ataaaaaaca aaacatatca gaacaggatc gaatcggtcc tgttcttttt ttgagaacac
    17881 aaaaagaaca aataccaaaa aaattttcct gaagttcact atttttatga tacaatatcc
    17941 aagataagga gggaacatgt tgaaagaatt aaaagtaact gacctcaaaa aaacttatgg
    18001 tgaaaaagat ttgtttgacc agatttcttt tttggtccac gaaaaagacc gtatcgggtt
    18061 gattgggacg aacgggacgg gtaaaacaag tctcttaaat attattgccg gtatcgatag
    18121 cagcgacggt gatcggcaaa cagtgtttta tccaactgat taccgtattg gttatctttc
    18181 tcaagcagct gaattttccg atgaattaac tgttctccaa gcagtctttc aaggaaacag
    18241 ccccttgatc caaactgttc gagcatatga ggaagcattg atcgaactag gggaaaacgg
    18301 agaagaccct gatgtgcaaa aacgttatgc taaagcagaa gaacaaatga ataaagaaga
    18361 tgcttggact acagatacca acgcaaagat tattttgcaa aaattaggaa tcagtgaatt
    18421 agataaaaaa atcagtactt tatcaggtgg tcagaaaaaa cgagttagtc tggcgcaagt
    18481 gttgattgat gaacccgatt tactattact agacgaacca acaaaccatt tagattatga
    18541 agcgatcgag tggttggaaa actatttgaa acaataccgt ggcgcactat tgatggttac
    18601 ccacgatcgt tattttctag atagagtagc aaaccgaatc ttcgaacttt catttggaaa
    18661 attatacgag tataaaggaa attatgaagc ttacgtctta gaaaaagcag aacgtgatcg
    18721 tgtagaagta gaacaagaag aaaagcgcaa gcggctttat aaacaggaat tagcttggat
    18781 gagggctggc gtacaggcac gtggcaccaa gcagcaagca cggatcaacc gtttcgaaga
    18841 tctaaaggag aacttgtacc aagtaaacca agaagatgat ttagagctga acctagcgac
    18901 acaacgttta gggaaaaaag tactggaaat caaagatggc agttaccgta taaatgacca
    18961 aacactgcta gaacatttag atttattgat tcaatctcgg gaaagactag gtattactgg
    19021 taaaaatggt gcgggaaaat cgacattgct taatattcta gctggcagaa ttcccctgga
    19081 tagcggtaca atgtctatcg gtgaaacggt tcatcttgcg tattatacac aagaaaatga
    19141 agaaatggca ccagataaac ggatgattgc ttacttacaa gaagctgctg aagaagcaaa
    19201 aacagcagat ggcagtcaaa tcggagtagc agaattactt gaacgatttt tatttccaag
    19261 atttatgcat gggacactga taggcaaact ttcaggcgga gaaaaacgcc ggttattttt
    19321 gttgaaactg ctgattcagc aacctaatgt tttattattg gatgaaccga ccaatgattt
    19381 ggacatagca acattgacga ttttggaaga ttattttaga agttttccag gtgctgtgat
    19441 cactgtttcc catgaccgtt atttcttgga caaggtagca gataagttat tagtattcca
    19501 aggaaacgga aaacaagaac tttattacgg aaacatgtcc agttatttac tgaagcaaaa
    19561 agagacccag cagcctgctg aaaaagcgaa accaaaaata cagagcaagg aacctgctga
    19621 aaagaagaaa ttatcttaca tggaacaaaa agaatgggaa acgattgaag atgagattgc
    19681 agaacttgaa gaaaaaatct cattgctcca agaagagatg aatcatcaag gagataattt
    19741 cactcgatta caagaattgc aaaacgacgt atctgaaaca gaagcacaac ttgaagaaaa
    19801 aatggcacgg tgggaatact tgagtgaatg ggtagaagat taggaggaaa aaggatggaa
    19861 caagcgtatc ttgacttagg aagaaaattg ttggaagaag ggcatcgtaa agaagacaga
    19921 acaggcacag gcacattaag cttattcgga taccaaatgc ggtttgatct gcaaaaaggg
    19981 tttcctttgt taacaacgaa acgagtacct tttggattga tcaaaagcga attgctttgg
    20041 tttttaaaag gagatacgaa tatccgctat ctattacaaa ataaaaacca tatttgggat
    20101 gaatgggcat ttgaacgcta catcaaaagt ccagactata atggaccaga tatgactgat
    20161 tttggccata gatgtttgac agatcctgaa ttcaatgaag aatataaaaa gcagagcaag
    20221 cttttttgcg agaagatcct aaccgatgat tcctttgctg aaacatacgg cgatcttgga
    20281 cacatctacg gctatcaatg gcgtcactgg gaaacaaaag acggtggctt tatcgatcaa
    20341 atcaaacaag tgatcgaagc aatcaaaaag actcctgatt ctagaagatt gatcgtttct
    20401 gcatggaatc cggaagatgt tcctagcatg gctttgcctc cttgtcatac gatgtttcaa
    20461 ttttatgttc aagagggacg gctaagctgc caattgtatc aacgcagtgg ggatgtgttt
    20521 ctaggcgttc cgttcaacat tgcaagttat gcgttgctga ctcacctaat tgcacatgaa
    20581 acaggtttag cggttggcga atttgttcac acattaggag atgctcatct ttatatcaat
    20641 catatcgaac aaatgaaaga acaattgagc cgagaaatac gcgcatttcc gactttagtt
    20701 ttgaatcagg aaaagcagtc agtatttgat tttgagatgg aagatatcca attagaagga
    20761 tatgacccac atccggcgat caaagctcct atcgctgttt agaatcagaa aggatgagaa
    20821 aatgttagct gcaatttggg cacaggatga aaacggattg attggaaaag aagaccgtct
    20881 tccttggcgt ttgccaaatg atctgaaatt cttcaaacaa atgacagaag caaatacact
    20941 agtgatggga cgaaaaacat tcgaagggat gggtagccgt ccgttgccga atcgtcagac
    21001 gattgttttg acgagagatt cttcctataa agcagaaggc gtgcatgtca tgcatagtgt
    21061 ggaagacgtg cttgctctgg aaaaagaaac agacggtatc tttttcatcg ctggagggtc
    21121 agctgtttat caagaatttc tgccattttg tacgatatta taccgtacag tcatccatca
    21181 tgtattcgat ggagatgctt attttccacc tgttgactgg tctgattggt cactgatcaa
    21241 tactagtcaa ggagaaatgg atgaaaaaaa tcgttatccg catcaatttg aaacgtatca
    21301 gcgtaatgag aatcctgtaa aaagcgggac aggcgaattg acgatataaa aaagctatgg
    21361 atagactagc tgttatttag agctgtccca tccatagctt tatttttttt attaagtaaa
    21421 gaataaaaca gagaagtaca taaaacctgc acctaacata acaaacaaat gccaaacaac
    21481 atgcatatag cggatagaat tcaagctata aaagaatgcg cctatcgtgt aagaaagccc
    21541 gcctgctacc atcaaaataa caccagtcat tccaagagag tgataaagag gaacagcagc
    21601 cacgacacag agccacccca taaaaatata gatgatcgta gatacttttg atactctctc
    21661 ccgtttatgc aacgtcaacg ctttatatat aatgccaaga atcgcaaaca accagattaa
    21721 tacaaataaa gtccaaccta gccatcctcg gatactcagc aaacaaaaag gagtataact
    21781 tcctgcaatc aaaagaaaaa tcgaactatg atcgaatact tggaacacct tttttgcttt
    21841 tgtaaaaatc aatgaatgaa acaaagtaga cgttaaaaat aaaagaatca tcatcgatcc
    21901 atagatagca taagagacaa tatggaggga ggaatgcaag tgggcacctt ttactaaaag
    21961 aaccactagt cccgcgatac tcaatccagt cccaattcca tgggtgactg cattaaacac
    22021 ttcatttaat atttcatacg ttcgattaaa tttcgttttt tccatgaaaa cggctccttt
    22081 actaggtgta attaatgaaa aatcattatt tgtctgttat actaaaaata gaaattgtct
    22141 tacatgtagt ttaacatgaa taaggcatct tgttaaagaa agagagagag aaaataatga
    22201 caaatgtaaa aatcgtaaca gattcttcat gtacgatgga aaaaaatgta cgcgatgctt
    22261 taggcattcg tgtcatgccg ctatccatca tgatcgacgg aacagtatat cccgatgatg
    22321 accaactaga cggcgaaaga tttatggaaa tgatggcgaa agcaaaaaat ctgcccaaaa
    22381 caagccaacc accaattgga gaattcatct cgatgtatga tgagctagga gaagacggca
    22441 gtgaagtgat ttcgatccat atgacaaaag gcttgagtgg aacagtagaa gcagcaagac
    22501 aagccagtaa tctaacaaaa acgaaagtaa cggttatcga cagtgatacc actgaccaag
    22561 gattatcttt tcaggtaatt cgagcagctg aattagcaaa agaaggtaaa acagtagaga
    22621 aaatccttga agaagtcaaa aaagtacaag acaatacaaa attatacatc ggaatatcca
    22681 cactagataa cttggtaaaa ggtggaagaa tcagccgaac aactggtttg ctgtctagtg
    22741 ttttgaacat acgcgtggtg atgaatttcg cccattctga attaatccct gtaactaagg
    22801 ggcgaggaaa gaaaacgttc gataaatggt ttaataattt gaaaaatgaa ctgaaagaat
    22861 tgccaaatgt gcgacaaatc ggtatttcac atgcggatgc aaaagaattg gcgcaagaat
    22921 tcaaagaggg tttgcaagaa ttattcccgg acatgcatat ccctgtactg catacgaatc
    22981 cggttatcgc tacgcatact ggtaaagacg cgtttgcaat catgtattat gtagattagt
    23041 cgaaaagagg ataagccctc agcctgaaag gcttgcagct tattctcttt ttctttataa
    23101 aattaggaaa ataattgttt tcttttttca taaagactac ctttttgata aaatttttga
    23161 taacattaga accaatcgaa tacaagaggt gaaaaaatga aattaacgaa acgacatttg
    23221 atcctcgttt tgttcccatt attaattgcg atcatcatct atgcaggttt agctgcttct
    23281 ataccgaaag ctggcccact aatgaaacca gtaactactg tgagagaaga tccaaataaa
    23341 aaagaaaaac ttcactacat agctatcgga gattcgctga ctgaaggagt aggagatcaa
    23401 accaagcaag gaggattcgt tccccttgtc gcaaatgatc taaaagaccg ttataatttg
    23461 accgcggttg aaacagaaaa ctatggtgta aatggtgaac gaagtgacca gatcttaaag
    23521 cgagtaaaaa caaaagaaga aattcgaaac aatatcaaaa cagctgattt tatcacactg
    23581 actgttggcg gtaatgattt gatgaaagtt atccaaaata atttattcgg gttaacggtc
    23641 aattccttta aaaagccact gaaaaactat caggaaaatg tcacagaact attggaagaa
    23701 tttcgatccc tcaataaaac cgcacctatt tacattttag gcatctacaa tccattttat
    23761 ttgaactttc ctgagatcac tgacatgcag acaattgtta acaattggaa caaaggaaca
    23821 gaaacggtga caaaaaaaat gccgaactgc tactttatcc caatcaatga tttgctttat
    23881 caaggattga atcaggaagt aggtgtcaca gaagaaggaa cagatcaaac aactagcacg
    23941 acagaaaatc taaatggcat caaaaataat gtgctctatg aagaagatca tttccatcca
    24001 aataatttag gttatcaatt gatggcaaat gcagtgagag atcagctgat cgagacacaa
    24061 aaccaatggt tagagaagga ggatgatcaa taaacatgaa aacaagatct gacgtatctt
    24121 caaacaaaac aaagaaaaca aattactgga aattcgcctt ccttatcctt cttggggtag
    24181 tacttggttc gaccgtattt ttaggcagcc gtatttttgc taatcgggaa ccagaactcc
    24241 cagaaatacc agctcttacg gaaagacaag gagatcctgt cttaacgatc aattcaaata
    24301 aagaaaaagt caatcaaatc atcagctttt tccttagtga gtatcaaaaa gatagtgata
    24361 ttgaatataa attttatttg gaaaatgaag cactactcaa tggtactttc gaagtactag
    24421 gttttcctat cgatttctat ttatattttg atccgtatgt gatggaaaac ggaaatgtcc
    24481 aactaaaagc tagaagtctt tctatcggaa cattgagttt gccaatcaaa gatgtgatga
    24541 atatgatcaa gagaaactat aaattaccag attggatcga agtcaataca gatgatctga
    24601 ctatcatgct taggttggat caattccgaa tgcaaaacgg gatgtatatt aaagcagata
    24661 aaatcgactt agtaaatgat gacattcggt ttagtctata tttgccaaaa gatacctctg
    24721 atgcttcaaa caactcaaat accaacaaaa ctagcaccag cagtactgaa agcaagacaa
    24781 aagaaaataa aaaatcatcg gatgagtagt attagagggg gaatcataat ggaacgagca
    24841 atatttgcag gaggctgctt ctggtgtatg atccagcctt tcgacacgct tcctggtatc
    24901 catacagtga tgtcaggata tactggaggg catgtaccag atccgactta tgaacaagta
    24961 aaaagccaaa cgactggaca tacagaagca gtggagatta tctatgatcc aacattgatt
    25021 tcatatgaac aattagtaga actttactgg cagcaaactg atccaactga cgctttcgga
    25081 caattcgagg atcgcggtga caattatcgc ccggtgatct tttatttgac agaagaacag
    25141 cggatcattg ctgaaaaaag taagaaacag ttagctgaaa gtggacgttt cgaacagcca
    25201 atcgttacaa aaatcgaacc agcccaacca ttttatctag cagaagaaga ccatcaagag
    25261 tattataaaa agcatcctga aaattttaaa agaaaccatg cacgccgggc tgcctttatc
    25321 gaagaaaatt ggaggaacga agggtgaatc gaagcttttt tcattattta atgacactgc
    25381 gaggcggtgg cacacatgat gctttatctg ttttcgctac agaagccggt aaagacatcc
    25441 aatttcctaa gcacagctct agctacgaag aaatttctaa ttatctggaa ctgaatgtcg
    25501 actatttgcc gtccatggat atctttgacg aagcatggga aaaatattta gaaaataatc
    25561 atgacatata agagatagtt ttgtacttta tgatcaaaaa agttttttgt ccaatagcct
    25621 aattgaaaga attattttta tttcagctat ctagtcgcat ttttttgata tttttcttat
    25681 aataaagaaa gaatgaaaaa aagagggatg gattgataga gattgacaaa aaaacaacgc
    25741 tatattaatc gcttctggct tgtattcaaa tacttacttg tttctgtatt ccttgcgttc
    25801 atgttgcggg gatttttatt catcccggta ccagtggaag gcaattcaat ggaaaacgtg
    25861 ttgaagcaag gtgatatggt tgtgatggaa aaattttcgg agatcagacg atttgatatt
    25921 gttgtttttc aattagcaga tggcacgatt tatatcaaac gagtaatcgg tcttccagga
    25981 gaaaatgtca gctaccaaaa tgatcaattg aagatcaatg ggaaagtagt taaggaacct
    26041 tatctaacta aaaacctgaa atcagatcat gcaaatgcat cctatacgac ggactttaca
    26101 ctacaagagc taacgggaca atccaagctt ccggaagata attattttgt acttggagat
    26161 aatcgaagag tttcaaaaga tagtcgttcg tttggcacga tcaataaaac tgatattcta
    26221 ggtaaagcaa gatttgtcta ttatccactt gatgaaataa aatggatcca gtgagaaaca
    26281 ataaagcgtg cagtctgttg agactgcacg ctttattgtt taatccaaaa tcagtttact
    26341 accttagtac gtaaaatcaa acagctcgtt taaacacttt tctagcctct aaagttttga
    26401 taaatgaata aacaaccatt atttgaatag ggatttggat catattcctg atgaatctac
    26461 ctagaaaaat tactttgaaa tctgcaccaa acaaaacaga catcagccaa gaattgataa
    26521 aaaggctgac tgggatcata atcaataatt gggcagcgat gacggaaggc aggtttatct
    26581 ttttatgata taaaaataag ccattaataa atccaccaag gaaagccgtg ataagaaaag
    26641 cagcttgaaa cgttcctgaa ccaaatatcg tgaaatttaa agtgttcgac aacatttcaa
    26701 gcaaaccagc ccagatcggt ccatatagat aacccataag cgccatactt aaaaatccga
    26761 atgtgatttg aaaataagca ggaccaatcg ctatcttgta taagatgaca tcaagggcta
    26821 ataaaagagc agaggtcgac aacaatcttg cattcatttc gggcaatcta aaaacgtctt
    26881 ttgcaatcaa agtgttcaaa atattctctc ctttgtcagt taataataaa aggataccat
    26941 aaaatcacca atgtgtcatg acacattggt gatttacata cactggcatc taacaattaa
    27001 aaaaagggtg agaagactgc ttttataaac tgtaaaaaag agattgtacg aataaaaaaa
    27061 atagaggcgg agaatactta tcgtatcatc cgcctttgtt tttttgttat aaaaaacgtc
    27121 agattagtta tctgcttaca tcttcatcca tacgggttct aatgtatttc cgtctagatc
    27181 ttggacttct aatgaaaaca tttcttcttc aggcattccc atatctactt cgtggaaagt
    27241 accaccattt tcttttgcaa tttgagcaaa tttttttact tcatccgcat tttcaacact
    27301 aaacgaaatc aaagcaccac ttgttttttt attatcagct atttctttat cttttataaa
    27361 ttttttataa aaatcatgag agagaagcat tatccaaaaa gtgtcatccc ataccattga
    27421 accagcttct tcagtagaaa attcttcatt ttttttaaaa cccagctttt cataaaaagc
    27481 aacggaacga ttgacatcag caacaggaaa attaacaaaa accatactat tcatttgttt
    27541 cgtctccttt ctgttttgtt cgtctatcat ctcttattta ggaaacgctg tcaaaaattt
    27601 agattcatat atagaaacca aaataaaaat ttgcatcatc aaaaaagtga agatgtgata
    27661 gaatgaggac tggttcactt tcacttaact aatagatgga atttctttat aataaaaaag
    27721 tgaatttata ttgaaaaaga aaaaaacaag taaagccggg gaatattccg gtatgaaagg
    27781 aatggagaga acaatgacca tccaatggtt tccgggacat atggcaaaag cccgacgaga
    27841 agtttctgaa aaaatgaaat atgtggacat cgtatttgaa ttggtagacg cacgattacc
    27901 tttatcttct cgtaatccaa tgctagatca gatcattcag caaaaacctc gccttgtttt
    27961 attgaataaa gcggatctgg cagatccaca acagacaaag ctgtggcaag aatattttcg
    28021 taaacaaggc catcttgcat tagctatcac ggcacaagaa agtaaaggaa ttaaaggtct
    28081 agtacctgcg gctaaagaag cattgaaaga aaaacgggca agagatgctg ctcgggggat
    28141 caagcctcgc gctattcggg caatggtctt aggtattcca aatgttggga aatctacgtt
    28201 gatgaatcga ctagtaggca agaaaattgc acagacagga aataagcctg gcgtaacaaa
    28261 aggtcagcag tggcttcgtt taggtaatga gttggaattg ttagacacac cagggatttt
    28321 atggcccaaa tttgaagatg aagaaatcgg caaaaaacta gcgctgacag gcgcgatcaa
    28381 agaccagctt gtccatttag atgatttagc gatttacgga ttagactttt ttgcccgata
    28441 ttattcagga agaatcagcg atcgttatgg tttatcaatc gagcaagaag aaaaattggc
    28501 accggaatta ctgatggata ttacagcaaa acgtgggtat aaagaagact atgatcgtgg
    28561 cagcgaatta gtcgtgcatg atattcgtca aggaaaactt ggacgataca cactggatcg
    28621 ttgcgaagaa atggagcaaa aaagtgatga ctgaatccat ccaaagtatc aaagaacgat
    28681 tgaaaagcat acagagaata gatgatccta cactagaatc attaagaaat gatccacgca
    28741 aaggcgtgca gcaagcactg gcttcttttg aacgacgatt gaaaaaggaa gcattaatta
    28801 aggaaaaata tgaagaaatg ttttgctttg aaaagcaagg gtacgcacaa ggtcaccaat
    28861 ggattgccgg cattgatgaa gtaggaagag gaccattagc tggacctgtc gtggcagctg
    28921 cagttatctt gccgaaaaat gagatcatat tagggctgaa tgactccaaa caattatcag
    28981 agaaaaagcg agaacttctt tatcaacaag ttcaagaaaa agccctggca atcgggatcg
    29041 ggatcgttga tcagcagaca atcgatgatg tgaatattta tgaagcaagt aaattagcga
    29101 tgatccaagc agtagaaaaa ttgtccattg taccaagcta tttgttgatc gatgcgatga
    29161 ccttggattt gcctattcag caggaaaaaa taatcaaagg ggatgcacga tctgtgtcga
    29221 ttgctgctgc aagtatcatt gctaaagtat atcgagacca attgatgaaa gaatatgacg
    29281 tgctttatcc tggatatggt ttctcgcata atgcaggtta tggaacaaag gaacacttag
    29341 aaggacttga acgacaagga attacgccaa tccatcgtaa aagttttgca cctgtaaaaa
    29401 aatatttaca ataaaaaaca gccgctttta cgtatcttat tagaaagaga gacgaaaggc
    29461 ggttttatga tgtatcaaat agaagaaaat ttattgaaac ttgcttatac aaaaggaatc
    29521 agtagtcaag gaaaatgggc acttgctaac tggatgatgc agtatcctta taaagaaatc
    29581 ggatttgaag acatcattaa gataggtaaa ataaccaaac accgggagct gtttcgcaaa
    29641 agttggcagg aaattcacgc taactggggc aaaatccaat cgaaacagtc gtttatcact
    29701 tgctttgatc ccaaatatcc ccctcagctg cttcacttga cctatccgcc aattgtactg
    29761 ttttataatg gtgatctatc tttactttct tgtaacatgt taagcgttgt tggtggaaga
    29821 cacacttctg ctttagccaa aaaaacagtt tattatttac taaaaccagt gatagaagct
    29881 ggatatgtga tcgtcagtgg ctgcgcgaaa gggatagata catacggtca ccaagcagca
    29941 atctctcatg gtggacggac aatcgcagtc attggaacag ggatagagca agtctatcct
    30001 gctgaaaatc gtgacttgca agaggtgatt gcaaatgatc atcttctttt gagtgaatat
    30061 cctcctcaat ccggcccgaa aaagcattgt tttccaatga gaaatcggat tatcgcagct
    30121 ttgtctaaag gaacatgtgt ctttgaagcc aagaaaaaaa gcggctctct gataacagca
    30181 cagcaagcat tggatcttgg aagaacagtt ttttctgttc caggaaatat tttaacagaa
    30241 cattctaccg gatgtcatca tttgatccaa gacggtgcag tttgcacggt ttcgggtgaa
    30301 gatatcttgg cagaatttaa agaataacac catacttttc atttgctttt cttgacaaac
    30361 gttttctgaa tggatatgat tagctacgat ttgtatgaat cctcttttaa tagtaggaac
    30421 acgacaatgg aaaggagtcc attcagtaat ggcgtataaa tatttagtga ttgtagaatc
    30481 acctgcaaaa gccaagacaa ttgagaaata tcttggacga aactataaag taatggccag
    30541 tgttggccat ataagagatt taccgaaaag taaaatgggt atcgattttg aaaacaatta
    30601 tgaaccccat tatatttcta tacgcggaaa aggcgatgtc atcaaaagcc tgaaagccgc
    30661 agcaaaaaaa gctcaaaaag tttacttggc aagtgacccg gatagagaag gagaagcgat
    30721 tgcttggcat ttagcgtacc tacttgggtt ggatctgaaa gaaaaaaatc gggtggtctt
    30781 caatgaaatc acaaaagacg cagtcaaagc agcttttaag gaaccaagaa cgatcgatgt
    30841 agatttagta gatgcacagc aagctcgtcg taccttagac agaatcgttg gttattcgat
    30901 cagtcctatt ctctggcgta aggtcaagaa agggttaagt gcaggacgtg tccaatctgt
    30961 cgctttaaaa attattattg accgtgaaaa agagatccga gaatttgttc cagaagaata
    31021 ttggagcatc gacggtaatt ttaaaaaagc tcgcaagaaa ttcaaagcaa atttctgggg
    31081 aatcgacggt aagaaaaaga aattaccaga tgcacaaagt gtaaaagaag tcactgctag
    31141 aattgatgga acagacttcg aagttaaaaa agtagaaaag aaagagcgca aaagaaaccc
    31201 tgcgaatcct ttcactacaa gtagcctgca acaagaagct gcccgtaagt taaactttcg
    31261 aacaagaaaa accatgatgg tagcacagca attatatgaa ggaatcactt tgggtaaaca
    31321 aggaacagtc ggcttgatta catacatgcg tactgattct acccggattg ccgatacagc
    31381 aaaagcagag gccgcagcat ttattgaaga tacgtatgga aaagaatttt ctagacatga
    31441 tcaccgaaag atcaaaaatg ctcaaggcgc acaagatgcc catgaagctg tgcgtccaac
    31501 gagtgtgctt cgtacaccag atgaactcaa acaatattta gataaagatc aattgaaact
    31561 ttatacattg atctggtcac gttttgtggc tagtcagatg acaccagcta ttttagatac
    31621 aatgaaagtg acattagaac aaaatggtgt catcttcatt gcgaacggtt caaaaatcaa
    31681 attcaaagga ttcatgcaag tgtatgtgga aggtagagat gacggcaaag aagagaaaga
    31741 aaatattctt ccagaacttg aagaaggcga tgtagtccaa tctgttgata ttgaaccaaa
    31801 acaacatttt actcaaccac cagcacgttt tagtgaggct acgttgattc gtgcactaga
    31861 agaaaacggg gtagggcgac cttccactta tgcaccaaca cttgatacga tccaaagacg
    31921 ctactatgta aaattgacac aaaaaagatt tgagccgact gaactaggag aaattgtcaa
    31981 ttcattaatc tgtgaattct tccctcaaat cgtcgatatc catttcacag cagaaatgga
    32041 aggagatttg gataaaattg aagaaggcac ggaagcgtgg gtcaaagtgg ttgatcgttt
    32101 ctataaaccg tttgaaaaag aactgacgaa tgcggaagaa aaaatcgaaa aaattcaaat
    32161 caaagatgaa ccagcaggat tcgattgtga tgtctgtgga catccgatgg tgataaaatt
    32221 agggaaatat gggaagtttt atgcttgcag taattttcct gattgtcgga atacgaaacc
    32281 aatcgtaaaa gaaatcggtg tgacttgccc agtatgtcat gaaggacaag tcattgaacg
    32341 aaaatctaag aaaaacagac tattctatgg ttgttcccgt ttcccagaat gtgaattcac
    32401 ttcttgggat aaaccagtgg gcagaaattg tccaaaatgt gacggatatc tggtcgagaa
    32461 gaaagtcaaa ggcggtaaac aagtagtctg tgtaaatgga gattacgaag aagacatcca
    32521 aaaataatag aaaagaggtt ctttcattga aaatggtaaa tgttgttgga gcagggcttg
    32581 ccggaagtga agctgcttgg cagatcgcac aagcaggagt ccctgtccgt ttatatgaaa
    32641 tgcgtccagt caaatcgact gaggcgcatc acacaagtaa ttttgctgaa ctagtatgtt
    32701 ccaattcatt aagaggaaac agtttggcta atgcagtagg gttattgaaa gaagaaatgc
    32761 gccgattgaa ttcggtagta atcacttcgg cagatgaaac agcagtgcca gctggtggtg
    32821 ccttagctgt tgaccgcgat tctttttctg aaacaattac ccgaaaagtc aaagagcatc
    32881 cattagtaac agtgataaac gaagaaatta ctgaaatacc ggaaggaatc accgtagtag
    32941 caacaggacc tttgacttct catccattag cagaaagcat caaagcattc aatggttcag
    33001 atggttttta tttctatgat gcagctgcac caatcgtaga caaagctacg atcgatatgg
    33061 ataaagtata tttgaagtcc cgctatgaca aaggagaagc agcttacttg aactgtccga
    33121 tgacagaaga ggaattcaaa gctttccatg aggcactaat caatgccgaa gtggcagaac
    33181 ttaaatcttt tgagaaagaa aaatattttg aaggatgtat gccaattgaa gtaatggcac
    33241 agcgaggatt caaaacgatg cttttcggac ctatgaaacc agttggattg gaagatccta
    33301 aaactggcaa acgtccttat gctgttatcc agctgcgtca agacaatgct gcagcttctt
    33361 tatacaacat cgtgggcttt cagactcact tgaaatgggg cgagcaaaaa cgtgttttcc
    33421 ggatgattcc tgggttagaa aatgctgaat tcgttcgcta tggtgtgatg caccgaaaca
    33481 gctttatgaa ttcgccagaa ttgctgaaac cgacttacca atcaacgaaa cgcgaagatc
    33541 tgttctttgc aggtcagatg actggtgtag aaggctatgt tgaaagtgca gcgagcggac
    33601 tacttgctgg aatcaacgcc gcacgcttag caaaagaaat ggaaccagtc gagtttccac
    33661 aagaaacagc tattggaagt atggcttact acattactca tgcggaagga aaacatttcc
    33721 aaccgatgaa tgccaactat ggtctgtttc ctgaactgcc agagcgcatt cgagacaaaa
    33781 aagcaagata tgaagcaatt gcacaacgag ctttaaaagc taatgaggca ataaaaacag
    33841 aattagaaaa aactgcaatc ccaaatgagt aaggaaactc catgaagagg gtgagacgga
    33901 tagtctcgct ctttttcttg cgtttttctt aagaatttct caaacaaaat gtgaggaatt
    33961 gcgccaattg tgaacaaact tagaaatatt ggttaaattc tgacaattta tttgtctttt
    34021 tataaacgct gtgctaaatt attctctggg acaaaaggaa ggtgagaaaa tgacagaagc
    34081 cacgtgggaa caaaaatttt tgcgttattt gattgtcgaa agaggatatt cagaaaaaac
    34141 gaaagaagca tatgaagaag acattctcca tttcaaacat tttttggagg aatctggaga
    34201 cgctgatctg ctgaaggcgg aatattttac ggttcgtgtc tatttgagtg cattagctga
    34261 tcatcgtcct agctatagca gaaattctat cagtcgcaag atagccagcc tacgttcgtt
    34321 ttatcagtat cttctcaaag aagaagtgat aaaagaaaat ccattttctt acgttcatct
    34381 caaaaagaaa aatttgagac tgccacgttt tttttatgaa aatgaaatgc aagcactttt
    34441 tgacagcgta gcaggagata gtcctttgga tttgaggaac cgcgctttac tggaagtcct
    34501 ttacgggtca ggcatccgtt taagtgagtg tagcagtttg tcggtatcag acattgattt
    34561 tgattcagag gtcatgttga tccatggtaa agggaacaaa gagagatatg ctcctctagg
    34621 ttcttttgct caagatgctc tgcaagaata tttcagggaa ggccgccagg tattgatgac
    34681 aaaatatcac gaagagcatg atatcgtttt tgtgaatcat catggaaaac caatcacgcc
    34741 tactggaatt gaatatgtct tgaatcaaat gatcaaaaaa agcagtttgg atagtgatat
    34801 ccatcctcac atgctgcgcc atacatttgc tactcattta ttaaataatg gggcagattt
    34861 gcggacagtc caagaattgc taggacatgc caatttatcg actacccaga tctatgccca
    34921 tgtaacaaaa gaaagtttac aaaaaaacta tcgttctttc catccaagag cgtaaaaata
    34981 aggaaaagaa caaatttagg aggaaacaaa catggtcgaa tcaccatttc attcaaccac
    35041 tatctgtgcc gtcgaaaaag acgggaaatt tgctatggct ggagatggcc aagtaacaat
    35101 gggtgaatct gttgtcatga aaggcactgc aaagaaagta cgacgtatct acaatggaga
    35161 agttgttgta ggtttcgccg gaagtgttgc agatgcgttt actttagaag aaaaatttga
    35221 aggcaaacta aacgaatata acgggaatct acaacgcgca gctgtcgaat tagctcaaga
    35281 atggcggaca cagcaatcta tgcaaaaact tgaagcgatg ctgatcgtaa tgaatgataa
    35341 agaaatgctt cttgtttcag gtacaggaga agttatcaca ccagatgatg gtatcttagc
    35401 tatcggatca ggagggaatt ttgcgttggc tgcagcccga gcaatgaaaa actatgctca
    35461 aaacgacatg agtgcgaaag aaattgcaga aaatgcgtta aatattgccg cagatatctg
    35521 tatctttact aatcataata ttatcgtaga agaactgtaa gaggtgagta gatatgtatg
    35581 aattaaaaca aacaccaaga caaatcgtaa gtgaattaga tgaatatatc gtaggacagc
    35641 acgcagcaaa aaaatctgtt gccgttgctt tgcgtaaccg ctatcgccgt ttgcagttag
    35701 atgaaaaaat gcaacaagat attacaccaa aaaatatgct gatgatcgga cctacagggg
    35761 tcggtaaaac agaaatcgca cgacgtttgg caaaaatcgt caatgcacca tttgtcaaag
    35821 tcgaagcaac aaaatttact gaagtcggtt atgtcggaag agatgtagaa tcaatggttc
    35881 gggatttagt agaaaatgcg atccaaatcg tagaaaaaga gcagtacagc cgtgtataca
    35941 caagtgctaa aaaaaatgca gaaaaacgtt tggtaaaaat cttggtacct ggtatcaaaa
    36001 aagaacagaa acaatccact aatcaatttg aacaaatgat gaatcttttt aatgctagtc
    36061 agcaaaatac cgctgaaccg caagaagaag tgacagaaga tattcgaatc aatcgccgta
    36121 cagtcttgga acaactacaa aagggtttac tggatagtcg tgaagtaacc attgaagttg
    36181 aagaccaaaa gaaacctgca ccaatgatga acaatggatt ggagcaaatg gggatcgacc
    36241 taaatgaaac tctaggtgct ttgacaccga agaaaaaagt ggaacggacg gtaacagtca
    36301 aagaagcttt agagttattg atcaaggaag aatcagcaaa attagtcaag gatgctgata
    36361 tccatagtga ggcaatcaaa ttagcggaag aaagtggaat tatttttatc gatgaaatcg
    36421 ataagatcac ttctaaatca cagcaatctg gagaagtatc cagagaaggc gtccaaagag
    36481 acatattgcc aattgtcgaa ggttcacaag taaacacaaa atacgggatc atccaaacag
    36541 atcacgtcct attcattgct tcaggagctt tccatttgtc taaaccaagt gatctaattc
    36601 cagaacttca aggacgcttc ccgatacggg tagaattaga tgatttgaca gctgaagatt
    36661 ttgtgaagat tctgaccgaa ccaaataatg ctttagtaaa acaatacgtc gcgttgatcg
    36721 gtacagaaaa tgtgtcagtt acgtttacaa aagaagcaat tgaacggatc gctcaaatcg
    36781 ctttcgatgt caaccgtgac acagataata tcggcgcacg cagactgcat accattttgg
    36841 aaaaactact agaagatcta ttatttgaag caccagatat gcaaatggga gaaataacga
    36901 tcacagaagc atatgtggat gacaaacttg gctcaattgc ggcaaatgaa gatttaagtc
    36961 gctatatttt ataaaaaatc ggaggacgac aatgacagac ttgctaacga aaacccgcaa
    37021 gataaacaca cttttgcagc aaaagaatac ttttgacatg caagcagatt tgccttacga
    37081 taaaatggct gtcacactag gtggtattct tgacagcaac acctatatca taggaagtga
    37141 cggagtatta ctaggttata atgaacgtca cgatgtgaac aatgaacgaa tcaaaacaat
    37201 gtttgttgag aaacaatttc ctgaatctta tactgataca gtggcacagt tgatgaaaac
    37261 tgaagctaat atcccaatta ctagtgatat tactgcattt ccggtagagt caagagagaa
    37321 atatccattt ggtctgacga ctgtcgttcc gatctttgga gctggcgaac gtttgggtac
    37381 gattatctta tctcgtatgg aacaatcgtt tgcagatgac gatctagtct tagcagaata
    37441 tggagctacg gttgtcggta tgcagatcct ttatcagaag tctcgaagca tcgaagcaga
    37501 tgtacggagt gcaacggctg tccaaatggc aatcaacaca ttatcttata gcgaattgaa
    37561 ggctgtacaa gctatctttg aagcattgga tggagatgaa ggtcgtctga ctgcttctaa
    37621 tattgccgat agtatcggca tcactcgttc tgtgatcgtc aatgctttac gaaaattgga
    37681 atcagctgga atcattgaat ctcgttcgtt aggcatgaag ggaacttatt taaaagtatt
    37741 gaattcccgt tttaaagacg agttagcaaa acacagttat tagaagtaag gggactgggg
    37801 cagatagaaa tgtttgttcc agccttccca ttttaaaaag taagtcgaag ttggaggaat
    37861 ggcaatgact gttattattc aaaatgacca gctgatcgcc gaaatcgctg aacacggtgc
    37921 ggaattgatc agtctgaaat caaaagaaca tggtttagag tatatctggc aaggtgatcc
    37981 agcgtattgg ggcagacatg caccggtttt attccctttt gttggacgtt taaaagatga
    38041 tcagtatgtt tatcaaggga aaacatatca aatggggcaa cacggatttg ctcgggatat
    38101 ggattttgaa gtcattgaac atggacaaga aatggctagt ttcatcttaa ggagtacgcc
    38161 ggagacatta gaaaagtatc catttgattt tgaattagtg atcagctatg aattaggagg
    38221 agacggcatc acggttcatt atcaagtggc aaataccggt gaaaaagaaa tgttcttttc
    38281 tatcggtgga catcctgctt tcaatgtacc tcttgaagaa ggattgtcat ttgaagattt
    38341 ctatctggca ttttctccat tgaaatcaag attacgccta ccattgaaag gcccattcat
    38401 cgatatggat caaaagacgt taggtcagac aaatacgaat ctggctttga atcgtgaact
    38461 ttttgagcaa gatgccttgg tttttgaaac aaggggtcta aatgcgtacg ccattcgaag
    38521 tgaaaaatct tcccatagcg taactttaag ctataaagat atgccttatg tagggatctg
    38581 gtctccttat ccaaaagaag cgcctttcgt ttgtatcgaa ccgtggtgcg gaatcgctga
    38641 cacggtgaac agtagtggaa acttgatgga aaaagtaggg atcaatcaac taggtgccca
    38701 tgaaatcttc aagacaaaat attccatcac agtgaagtaa gaggttgtga ccgagaagtt
    38761 agcagggaac accgtttacc aaggtgtgta aaaaagctat cctgtaagta ataagggagt
    38821 gtgacatcga tttgtcacac tcccgtttta tctctttgga atgaaaaagt atcaggattt
    38881 tttcttgctg ctattcaaac caaacgggat tcgactttca gtatggtttc tgatacgctt
    38941 gatattgtct ttatgccgat aaaagataaa ggaagaaact aaaaaggcaa tgatcgtcag
    39001 taaccaattg ggcttagcca aaatcacagg agctacataa ggaaggacga tcgtagataa
    39061 agtgatcaat actgcactga tcatactgct caaactcacc atacttgtac aaaataaact
    39121 aacgataaag atgatacaag aataaacaaa aaatacagga ttaaatccta aaagcattcc
    39181 tgcacttgtc gcaacagctt ttcctccttt gaacccagca aaaatcggaa atgtatggcc
    39241 aagaatggcg cataccccaa accaaagagg gttaatccca gaaaggtgga agatcatcgg
    39301 caaacaagta gccaatgtac cttttaaaat atccataagc aggacaacaa tcccggctgt
    39361 tttgcctaat acacgaaatg tattggtcgt tccagtatta ccgctcccgt gttgtcggat
    39421 atcttttttg aaaaacaatt tgccaatcca gacaccggaa ggaatagaac ctaaaagata
    39481 tgctaaaata agtaataaga tcaatttcat tttttaccct ctttcaagtt gaaaactatc
    39541 ctttatttta gcatgaatag gtaacaggaa caattgaaaa aatgaaagat acaagtataa
    39601 tagatggaat aaaaagaaaa gcaaggggtg gcagaaatgt ctgtaaaaaa tccaacacaa
    39661 gaaacgattt tcaactattt ggaagaagca aaaaatattg ccgtcgtagg cttgagcaat
    39721 aaaccagaac gaacaagtta catggttgca caggtattgc aagacaatgg atataaaatc
    39781 ataccagtaa atccattatt ggctggtgaa gaaattctag gcgagaaagt ctatgcgaat
    39841 ttgacagaaa tcgatgatcc aatcgatatc gtagatgtat ttagacggag tgaattttta
    39901 cctgaaatag caggtgattt tttgaaaact gatgctaaag tgttctgggc acaattaggg
    39961 attgaaaaca aagaagcagc tgagatgcta agaaaagcag gacgtacaga tatcgtaatg
    40021 gatcgttgta ttaagattga acttggtaag ttgaaagaaa accaaaaata aaaatgacca
    40081 caaagaagag atgatcgata gaggggaatg tcgaaaaagc ggttaggaga taattaaaat
    40141 tgatttctcg tctaccctct tttttttcac gaccttttct agtatgataa tcgtggtgcg
    40201 gttttgccgc aaagtattat tgaaggagcg tttgccttgg caaaaaaagt aaacaacgaa
    40261 tacaatgact cgtccatcca agtattggaa ggattagagg cagttagaaa aagaccaggg
    40321 atgtacattg gatccactga cagccgaggc ttgcatcatt tagtctatga aatcgtcgat
    40381 aatgcagttg acgaagcatt atcaggttat ggcagtgaga ttgatgtgac catccatgaa
    40441 gacaatagta taactgttgc agatagcgga cgtgggatgc ctgtaggaat gcatgcttcc
    40501 ggaataccaa cagttgaagt gatttttact gtgctgcatg ctggcggaaa gtttggtcaa
    40561 ggagggtaca aaacttctgg tggactgcac ggtgtaggag ccagtgtggt aaatgcctta
    40621 tcgaaatggc taattgtaac gatcgttaga gatggcgtag agtatcagca gaaatttaaa
    40681 aacggcggta aacctgacgg tactttgaaa aagataggaa aaacaaaaaa agcaaacggg
    40741 acgactgtcc attttttacc agatgataca atattcagta caacgaaatt ttcatatgaa
    40801 attttagccg aacggttaag agaatctgcc tttcttctaa aaggcgttaa aatctctctt
    40861 tctgatcttc gtggagaaga accagtcaag gaaatttttc attacgaaga aggaatcaaa
    40921 gaatttgttg attatctgaa tgaagaaaaa gatacattga caccagttgt ttatttttca
    40981 ggagaaaaag aaggtatcga agtagaggtc gcttatcaat acaatgacgg ttattcagaa
    41041 aatgttttat cttttgtcaa taacgttcgg acaaaagatg ggggaaccca tgaagctggt
    41101 atgaaagcgg cgatgacgaa atcttataat gaatatgcga gaaaagttgg tttgctgaaa
    41161 gaacgggaca agaatctgga aggtagtgat ttcagagaag ggttagctgc tgtactttct
    41221 atccgtgtac ctgaaaatct tctgcaattt gaagggcaaa caaaagaaaa acttggtacg
    41281 ccagttgctc gtaccgtagt agataacgtc atcagtgaac aaatgggctt ttatttgcag
    41341 gaaaatagcg aaatgagcca gatgttggtc cgtaaagcaa tcaaagccag agaagcgaga
    41401 gaagcagcac gtaaagccag agaagaaagc cgaaatggga agaagcgtaa aaaaggcgaa
    41461 tctcttcttt caggaaaatt gacacccgca caatcccgaa accctaagaa aaatgaattg
    41521 tatttagtcg aaggggattc tgctggcgga tctgctaaac aaggaagaga cagaaagttc
    41581 caagcaattc tacctttacg aggaaaagtc atcaataccg aaaaagctaa aatgcaagat
    41641 atcttgaaaa atgaagaaat caatacgatg atctatacga ttggtgcagg agtcggtcct
    41701 gagttttcta tcgaagactg taattacgac aaagtaatca tcatgaccga tgcggataca
    41761 gacggcgccc atattcaagt attactgctt actttctttt atcgttatat gaaaccgctg
    41821 attgaagctg ggaaagtata tatcgcgtta ccgcctttat ataaagtatc caaaggacaa
    41881 ggaaagaagc aagtcattga atatgcgtgg acagatgacg aattggcagc aatgattaaa
    41941 aaagtcggca aaggatatat gctgcagcga tataaaggac ttggagagat gaatgcggaa
    42001 caattgtggg aaacaacgat ggatccgact tcccgtacgt tgatccgcgt ccgtatcgat
    42061 gatgctgcgc aagctgaacg tcgagtaacc acactgatgg gtgacaaagt agaacctcga
    42121 agaaaatgga tcgaaaatca cgtgcaattt acgctcgaag aagatggcag tatcttagat
    42181 aaaaaagagg atactgaaat ctctccttca gtttctaatg atttattaga tgaagaacga
    42241 gcagataaaa atgaaaacaa tcagttattt gaagtagaat aggaggtcac ctgatggaaa
    42301 atcgacaaga agtacaagaa ttaactttag aagaagtgat gggtgaccgc ttcggacgtt
    42361 attccaaata tattatccaa gaacgtgcac tgcctgatat ccgtgatgga ttgaagccag
    42421 tgcaacgccg gatattattt gccatgaaca aagatggcaa tacgtatgac aaaggattcc
    42481 gtaaatcagc aaaatctgtc ggaaatatca tggggaatta tcatcctcat ggtgacatca
    42541 gtatttatga agcgatggtc cgcatgagcc aagactggaa gttgcgggaa gtactgatcg
    42601 aaatgcacgg gaataacggg agtatggacg gtgatccacc tgctgcgatg cgttatactg
    42661 aagccagatt atccaagtta agtggagaga tgctagcaga tatcgagaaa gaaacagtcg
    42721 atctcgtgtg gaattttgat gatacagaaa aagaaccaac agtccttcca gctcgatacc
    42781 caaaccttct cgtcaatgga tcgacaggta tatctgctgg atatgcaaca gaaatcccga
    42841 ctcataactt agctgaagtg atcgatggca caatctatat gatcgatcat ccacaagcga
    42901 gccttgaaaa attgatggaa tatattcctg gacctgactt tccaacagga gggatcttgc
    42961 aaggaaaaga tgaaatcaaa aaagcttatg aaacaggacg aggaaaagtt attcttcgtt
    43021 cgaaaacaaa gatagaggca atgaagggta ataaacagca aatcgtgatc acagaaatcc
    43081 catatgaagt aaataaagcg accttggtga aaaaaatgga tgagatccgt ttgaataaga
    43141 aaatcgatgg aatagctgaa gtacgagatg aaagtgatag aacaggtctg caaattgtcg
    43201 tcgaattgaa aaaagacgcc aatgctcaag gaattttgaa ttatttgttc aaaaacactg
    43261 agctacagat caattacaat ttcaatatgg tcgctatcga ccatatgact ccgcatcaag
    43321 ttggactgaa agacatttta agaagttata tcgagcatcg aaagcaagtc ataacaaaac
    43381 gtagtcaatt cgatttagcc aaagctcaaa aaagacagca tatcgtagaa ggattgatga
    43441 aagctttatc gattctggat gaagtaatcg caacgattcg tgaaagtaaa gacaaaaaag
    43501 acgccaaaaa gaatttagtc gacgtctttc aatttacaga agaacaagca gaagcaattg
    43561 tcacattgca attataccgt ttgacgaaca cggatatcac agagcttcaa aaggaatccg
    43621 aaagcttgat tgcgcaaatc acagagttga ataaaatcct ttcaaatgac aaagagcttt
    43681 tctctgttat gaaaaaagaa ttgcgagaag tgaaaaaaaa ttattcttcc gctcgtctaa
    43741 cgacaatcga agacgaaatc gaagaaatca aaattgatac gcaagtctta gtggcacaag
    43801 aagacgtaat tgtcagtgtg acgcgagaag gctatgtgaa gcgaacaagc cttcgttctt
    43861 atagtgcttc caaaccagaa gaaatcggga tgcgagaagg cgactacttg ctatactcag
    43921 gtgaattgag tacattggac catgttcttc tgatcacaaa taaagcaaat gtgatctacc
    43981 gccctgtcca tgaactaccc gatttgaaat ggaaagatgc tggtgagcac atttctcaaa
    44041 caatctccaa tctgtctgta gacgaatcga ttttagctgt tttcccatat caaaaaattc
    44101 aagcagagaa aacatttgta ttcatcagca agaatggatt gatcaagcaa acacgaatga
    44161 cagagttcga accatggcgc acctataaga gccgtccgct cagctgtatg aagctaaagg
    44221 cggaagaaga tgaactgatt gctgtgtatc tgaaagaaga tcagccaggc ttagatgttt
    44281 tcttagtaac gcatcaagga atgggtttgc gctatccttt aacagatgtt cccgttgttg
    44341 gagcaaaggc agctggagtg aagtctatga atttgaaaga tggagatagg attatcaacg
    44401 ggctgcttgt gttagcagaa ggtgatacgc cgatcgtaat cgttactcag cgaggagctg
    44461 ttaaacgcat gcttgctcaa gaaatcagcc aaacttcacg tgcgaagcgg ggagtgacgg
    44521 tcatgcgtga gctgaaaaaa aatccgcacc gtattatcta tatgtcggaa ggaagatcaa
    44581 aagaaataac cttgatgaat caaaaagggc agcagttaac gattgaccca actgatttcc
    44641 ctataggtga tcgaacatcg aatggatcat ttgcgatgga tgagaaaaaa ggtggagaag
    44701 tcatcgaagt tatcgatgct cctgagattt tcatcgagtc gtaactgttg tagtatttta
    44761 atgaagagac gccatctgta ttaatacagg tggcgtctct ttttcattca tttttaataa
    44821 atccttatac gtttgcaaaa aaagatgaaa gtgttcacaa tgcttaaatg ttggtatgtc
    44881 aacgttgatt taaatatatg taaataaaac ataaaaattg ttaaataaaa aacaagaaaa
    44941 cccttgcaaa gatagggtgc tgtgttatac taatcacgta aaaactgtta cataaaacag
    45001 aaatttagga ggtccataca atggagcaat ggaatggatt taaaggtacc aaatggaaag
    45061 aaggcgtaga cactagagac tttattcaaa ataactacac tgaatatcgc ggtgatgaca
    45121 gctttttgga aggcccaaca gaagcaacag aaaaattatg ggatgaattg caaaagttaa
    45181 atgatatcca attcgagcgt aacggtgtct atgatatgga cacagaaatc gtatcaacta
    45241 ttacttcaca cgatgcaggc tatttggata aagatttgga aaaaatcgtc ggtctacaga
    45301 cagacaaacc tttgaaacaa gcattcatgc cgttcggcgg aatcaatatg gctaacaatg
    45361 cgctaaaaag caatggctat acaccaaacg atgaattgac acatattttc actgactggc
    45421 gcaaaacaca taatcaaggt gtatttgacg cttatactgc tgaaatgcgt gctgctcgta
    45481 aaaacaaaat cattacaggg cttccagacg cttacggtcg cggacgtatc atcggtgact
    45541 atcgtcgtgt ggcattgtat ggaatcgact atttgatgga acaaaagaaa aaagatcatg
    45601 atatgactgg ttatcataca atgagtgaag aagtgattcg tttacgtgaa gaaatcactg
    45661 aacaatatcg tgcattagcg caaatgaaag aaatggctgc gaaatatggc tatgatattt
    45721 ctaaaccagc tgcaaatgca aaagaagctg tccaatggct atacttcgga tatttagcag
    45781 caatcaaatc acaaaatggt gctgcgatgt caatcggacg tatctcagca ttccttgata
    45841 tctatatcca acgtgatttg gataatggag taatcacaga aaaagaagca caagaattga
    45901 tcgaccactt agtaatgaaa ttacgtatgg tcaaattcgc gcgtacacct gaatataacc
    45961 aattattctc aggattccct atctgggcta ctttatccat tgcaggtatg ggattagatg
    46021 gtcgttcatt agttacaaag aatgatttcc gtctgcttca tacattaaca aatatgggac
    46081 cttcaccaga accaaacttg actgttttgt attctgaaca ccttccagaa ggattcagaa
    46141 catatgctag cagaatcgct atcgaaagtt cttcgattca attcgaaaat gataatcagc
    46201 ttcgtgaaca atggggttct gatgattgtg cgattgcttg ctgtgtatca ggaacagttg
    46261 tcggaaaaga catgcaattc tttggcgctc gtgctaactt ggctaaaaca ctattatatg
    46321 ccatcaatgg cggagtcgat gaagtgactg gcgcccaagt tggaccagaa tatcgtccaa
    46381 tcacatcaga aatactagac tttgaagaag tgaaacataa cttcatgaat atgatggatt
    46441 ggttagcaga actatatgtc aatacgttga acatcattca ttatatgcat gataaatatt
    46501 cttatgaagc agctcagctg gctttgatgg attctgactt gcaacgcaca tttgctacag
    46561 gtattgctgg gatctctcat gcggcagaca gcttgtcagc gatcaaatat gcaaaagtaa
    46621 aagcaatccg taatgaaaaa ggaatagcag ttgattttga agtggatggc gacttcccta
    46681 aatacggaaa tgatgatgac cgtgcagatg aattagtttc atggatcttg gaatacttca
    46741 tgggacaaat caaacgtcat aaaacttacc gcaactcaaa accaacaact tcattattga
    46801 cgattacttc aaatgttgtt tacggtaaaa atacaggaaa tactcctgat ggccgtgaag
    46861 ctggaaaacc acttgcgcca ggagcaaatc caagttacaa tgcggaacaa agtgggttat
    46921 tagctagcct gaactcaaca gctaaaatga attacggctg tgcattggat ggaatttcaa
    46981 atacccaaac aatcaatcct tcaggtcttg gaaaagaaca agatacaaga atcgaaaact
    47041 tgcgtaatgt cttagatgga tacttctcaa aaggagctta tcatttgaat gtcaatgtat
    47101 tcagtaatga tttattgcgt gatgcaatgg aaaatccaat gaaatatccg aacttgacga
    47161 tccgggtatc tggttatgca gttaaattcc gtgacttgac aagagaacaa caattagatg
    47221 tcttgatgag aacttcacac gacagaatgt aaaaagttcg tgaaaaaagt ttttcgactt
    47281 agcgtcgaaa ggacgttgaa tagccatcgc atcaaaatcg aataatcagt atgcttattt
    47341 gaaagaaacg atttacaggg gcaggacttg tatgttccgt ccctgtaaat tttacacata
    47401 acgaagcggc aattgcgaaa aattttgttc agaaagcttc gcttcgacag ctaacttgta
    47461 cttaaaatga aatcaaagaa aagatgtgaa cttaaatgga agaaaaaaca atcggctatg
    47521 ttcattctat cgaaactttc ggttcagtag acggacctgg actgcgcttt gtcgtgttta
    47581 tgcaaggatg ccgcatgcgt tgccaatttt gtcacaatcc agatacttgg aatattggcg
    47641 gagggaaaga atacacagca gatgaacttt tagataaagc agaacgcttc agaccttact
    47701 ggggagataa aggtggtatt actgttagtg gtggagaacc tctattgcag atcgattttt
    47761 tgatcgaact atttaaaaaa gcaaaagagc ggaagatgca tactacattg gatacatgcg
    47821 gaaaaccttt tacgtatgaa gaacctttct tttcacgttt tcaagaattg atgaagtata
    47881 cagatttact gttatttgac atcaaacaca tcgataatga agagcataaa aaactaacgc
    47941 attgggacaa tgaaaatatt ttagaaatgg ctcaatattt gtcaaaaatc aacaaaccgg
    48001 tttggattcg ccatgtacta gttcctgagc gaagtgatta tgatgaatat ttgatccgac
    48061 tagacaactt tattcaaacc ttgtcaaatg tagacagagt agagattctt ccttatcata
    48121 cgatgggaaa atacaagtgg gaaacacttg gtttgaaata tccattagaa gggatcgaac
    48181 cacctacaaa agaacgtgtc gaaaatgcta aacgattgct tcatacagaa gagtatacaa
    48241 aatatttaac gcgataggtc gcatcggaaa gaagataagt attgcttctt tccgtttttt
    48301 tgctattatt aaataggaga aatcacaaag gagcgaatga catgtcaaaa attttagttt
    48361 ttggtcacca aaatccagat acagatgcaa tcggggcagc cattgcgttt tcacacttgc
    48421 aaaatgaatt aggtaaagaa acagaagcag ttgctctagg tacacctaat gaagaaacac
    48481 aatacgcatt agaccacttt cagctatctg caccacgagt agtagagtca gctaaagcag
    48541 aaaatgcaga tgtcatgctt gtggatcata atgaattcca acaaagtatt tcagatatca
    48601 gcgaagtaaa tattttagct gtagtagacc accatcgtat tgcaaacttt gaaacagcta
    48661 atccattgta ctatcgtgca gaaccagttg gatgtacaag tacgattatt ttaaaactat
    48721 ataaagaaaa ccaagtaaca attcctaaag aaatcgcagg aatcatgctc tcagctatca
    48781 tttcagatac attgttgttc aaatcaccaa catgtacgcc tgaagatgaa gcagctgcaa
    48841 aagaacttgc agcaatcgct gaagtcgatc taaacactta tggacttgaa atgttgaaag
    48901 caggaacaaa tctaagtgat aaatcagctg cggttcttct tgatttggat gcaaaaagtt
    48961 tcccaatggg cagttccaac gttcgtatcg ctcaagtcaa tacagtggac cttaatgaag
    49021 tgatggatcg acaagcagag ctagaagcag caatggaaga tgaaatgaaa gcaaatggct
    49081 acgatctgtt cgtattagtt gtcacaaaca tcttagacag tgattcagaa ttacttgttg
    49141 ctggtaaagg aaaagaaaaa gtagaagaag ctttccaaac aactttgacc aacaaccgtg
    49201 ctttgcttaa aggtgtcgtt tcacgtaaaa aacaagtcgt acctcaattg acggctgtat
    49261 ttagctaatc aaatggtagt atgaagcatt caaaatgaat ggataagcga ttgatataaa
    49321 aagaaacata gaacatacta aaggccgaag aaatgggctc tttgtcaata aggactgatg
    49381 agttgtgcaa
//
LOCUS       DACGLE010000212        20984 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0534
            SAMEA3304449-rid9560453.guided.044, whole genome shotgun sequence.
ACCESSION   DACGLE010000212 DACGLE010000000
VERSION     DACGLE010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304449
            Sequence Read Archive: ERR985064
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20984)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20984)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702225.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702225.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 20:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,045
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 27 of 67
            Pseudo Genes (incomplete)         :: 25 of 67
            Pseudo Genes (internal stop)      :: 19 of 67
            Pseudo Genes (multiple problems)  :: 4 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20984
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304449-rid9560453.guided.044"
                     /strain="VREN0534"
                     /db_xref="taxon:1352"
     gene            complement(134..577)
                     /locus_tag="IXT20_002946"
     CDS             complement(134..577)
                     /locus_tag="IXT20_002946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HAQ4861305.1"
                     /translation="MKKYWKFLLMIGVSTVVMFGMMYLNVYALDHIFFSRTRVFMALM
                     MGAVMAVIMLLFMWKMYENKKINVMILAASLLVFAGSLFMVRSQTTVNDTAWMKAMIP
                     HHSIAILTSKRADLKDPEVKKLAEEIIQAQEEEIDKMKKMIKEME"
     gene            complement(795..2135)
                     /locus_tag="IXT20_002947"
     CDS             complement(795..2135)
                     /locus_tag="IXT20_002947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ4861306.1"
                     /translation="MEDKKLKKQLSSRHITMLALGGAIGAGLFKGSGEAIGIAGPSVL
                     IAFLIGGLILYIVMKGLGKIVLSGGDTHHGLSGLIRPYLGAHSADFTDWVYWSMWIIN
                     IIAEAVAAASFLQLWFPHIPAWVFVFMLAVLTTIINLYSVRLFAETEYWLAFAKISVI
                     ILLIIFATYLVGQQMLGTGVFPTLQQLTDHGGFTPHGMKGIISSLLVVIYSYGGSELI
                     AITVSEADDPKRAIPKAIKGVMGRIISFYIIPLFLLLILFPWNTLAGTNVSPFVMVFE
                     KMNIPFAADIVNFVIVLALFSSINSGVYASSRILFFRLKDRKGSSRKLAVLNKHQVPQ
                     RAVLFCTSVLYLGVALSYFVGDKLFGYLAGSLSYTVLLIWIIISAASFVLALRKGTLW
                     DKGISFFALAVLGLIFLGILFTNSIGVTVLTGLLYLFIYFSYQKKNDAFVLTNE"
     gene            2404..3300
                     /locus_tag="IXT20_002948"
     CDS             2404..3300
                     /locus_tag="IXT20_002948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HAQ4861307.1"
                     /translation="MNKNDVLLVAKNKSLYFLPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFNMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMDFSSSEPKTDLFFGATNNGRKERAESYKQEFIKR
                     DVTTNYFILPHRGNEQPGYLAYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYHFYRPENIFLLRERDLDELPEFLHTPFKPIPSELLEFYDAENWAKR
                     FITNDSTIYEQYEYDLSSVENK"
     gene            complement(3450..4391)
                     /locus_tag="IXT20_002949"
     CDS             complement(3450..4391)
                     /locus_tag="IXT20_002949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HAQ4861308.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFRSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEAKLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKTAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAVWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            4516..5637
                     /locus_tag="IXT20_002950"
     CDS             4516..5637
                     /locus_tag="IXT20_002950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HAQ4861309.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALGGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPHGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            5895..7397
                     /gene="lsa"
                     /locus_tag="IXT20_002951"
     CDS             5895..7397
                     /gene="lsa"
                     /locus_tag="IXT20_002951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lsa family ABC-F type ribosomal protection
                     protein"
                     /protein_id="HAQ4861310.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKSQPKHLSISYVRQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            7464..7991
                     /locus_tag="IXT20_002952"
     CDS             7464..7991
                     /locus_tag="IXT20_002952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HAQ4861311.1"
                     /translation="MLYVTRHGETTWNAQGLVCGRADVPLTEKGQMQAQKLAEKVVDL
                     PVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYDGLPSKDENFQKAR
                     LAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIRVINAYFHPMPNEG
                     FFTFMVDNTELVSYE"
     gene            8202..9263
                     /locus_tag="IXT20_002953"
     CDS             8202..9263
                     /locus_tag="IXT20_002953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HAQ4861312.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQKRLIELGSEAKKNKLELMVDISGEALERAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPKDIDELKEAEADFSQIEAWHNYYPRPETGLDKEWYQKK
                     NQWLKIQGLLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSENVQEQFASFQKEQTVLLHTEPVDEEFYEYILGKHTNRQDDARDVIRSADARF
                     REIPPIPARNTATRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            9266..10162
                     /gene="murQ"
                     /locus_tag="IXT20_002954"
     CDS             9266..10162
                     /gene="murQ"
                     /locus_tag="IXT20_002954"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HAQ4861313.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELTSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISKHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEEAQAKLAEANGFVKGTL"
     gene            10176..12158
                     /locus_tag="IXT20_002955"
     CDS             10176..12158
                     /locus_tag="IXT20_002955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ4861314.1"
                     /translation="MAKNELTLEELAKQIYKGAGGMGNVESIVHCMTRVRMKIINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQQMVDLAGVKLGEKLSSVSAATAN
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAATWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALAAGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKVVPQTSA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVVSSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAAGK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(12223..12669)
                     /gene="nrdI"
                     /locus_tag="IXT20_002956"
     CDS             complement(12223..12669)
                     /gene="nrdI"
                     /locus_tag="IXT20_002956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HAQ4861315.1"
                     /translation="MNILYISISGNTRAFAKHLAEYAEKMHAEDPVNPEVTLKEIHEN
                     SDFTKETEPFFTFVPTYLDGGNGLDNGDTEILTETMREYLEHEDNHKLCLGVVGSGNK
                     NFNNQYCLTAKQYAQTFGFPFLADYELRGTPSDVTRIYQILAENNK"
     gene            12880..14304
                     /locus_tag="IXT20_002957"
     CDS             12880..14304
                     /locus_tag="IXT20_002957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ4861316.1"
                     /translation="MDTEQQKKGIGLIPLAALVIGSAIGGGVFGIMTDISGSAGGPAI
                     FSWVLVGTAMLMLSLSINNINKKRPDLKGGIFSYAEEGFGRFAGFISGWGYWLTCWLG
                     NVAFATLLMSALGYFFPVFEGGQNLASIVMSTVFLWGYAWLVNRGVENASFVNAIVTF
                     CKLVPLFLFIVVAISTFSIQQFIDNFSSDIILNANGQPIPFSSQVLSCIMVMLWVFVG
                     IEGASVVGSRAKKQSDVGKATILGIFGLIIIYVLVSMLPYGTMTMNQLQTIKAQPAMG
                     YVFEMMVGKWGAVVINGGLIISLVGVWLSWTILPIETMGNMAEDGLLPKAWGKLNKNG
                     APTYAIVLTTLCTNVFLLSLLFTDSAYNFAYTLGTAAIFFTWLFLGLYQMKLSYQRKE
                     WVQFLVGLLASAFQVWAMLVVAFNEVMLVLVLFLPGIYFYLKARKEQNEPVQHIHLDK
                     ILLIGVTMVGVIAMVLFATGMLHV"
     gene            complement(14485..15258)
                     /locus_tag="IXT20_002958"
     CDS             complement(14485..15258)
                     /locus_tag="IXT20_002958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAQ4861317.1"
                     /translation="MTLLVKQELFKLIKKKSTAVLSVLLVVLLIGTALLAKKYTTIID
                     PVEMTAQLFSATSWIVFIMIAAASTIISMEAQYGTLKNLLYRKYSRGEILVSKWITLV
                     IYSVYLYLLAIIVTVLMKLILFPSISFTEKVSTGQTLIQSLFTYTLGSYIGLWLILSL
                     VLMIACFINSSGASISAGIVFYFASSIISGILIALIQKWEWIKWNPISMLNLQNQIGN
                     EEIMKQLTHLSTNQMLFGNLAYIVLFLALGYLVFKRKNV"
     gene            complement(15255..16160)
                     /locus_tag="IXT20_002959"
     CDS             complement(15255..16160)
                     /locus_tag="IXT20_002959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ4861318.1"
                     /translation="MKSLSVQELSKNFGHKQALSDVSFSLSPGKIVGLVGPNGAGKTT
                     IMKAILGLIHYSDGTICIGENEISPSSHKGLEQVGALIEYPGIYPFLTGYDHLKLFSE
                     TNDVSAIDTIVNQLKMEKYIHKKAKSYSLGMKQKLGIALALLNHPSIVILDEPMNGLD
                     PQATRDVRKIITDLATQGTSFLISSHLLSELEKMVDELILINQGKIIKQCTMAELNQT
                     AQDFIVISTTNDLAARTILTEAGYTLEDPDKIKLLKENEELLGTVIQLLSAQNIHVTD
                     VQHLTNDLESSLLEILDSVKGEEEK"
     gene            complement(16314..16634)
                     /locus_tag="IXT20_002960"
     CDS             complement(16314..16634)
                     /locus_tag="IXT20_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HAQ4861319.1"
                     /translation="MAWMELIIAGILEVFWSTMMKWSDGFSKINYSFYTVIGMIASFY
                     FLSKAIKSLPMSLSYPIWTGIGAVGSVIIGVVLFHDKLNLSTWFFVGMLLISIIGIKI
                     TSGH"
     gene            17108..17794
                     /locus_tag="IXT20_002961"
     CDS             17108..17794
                     /locus_tag="IXT20_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HAQ4861320.1"
                     /translation="MENYLSLLGVLIVILGFAFKLDSILIVMVALVVTALTGGLGIQG
                     MLELLGTSFVNNRGMAIFIIIMLATGTLERNGLKESAASLIKRFKKVSAGMIVDIYGI
                     FRMIFAAFNVSFGGVAGFVRPIILPMALGTIESKGLEVHPKHEEELKGMASAMENVCW
                     FFGQVLFIGGAGALLVQSTLKDLGYDVTLGQLALVEVPVAITALVVASVYFYLKEKKL
                     AKKYYPGKGQ"
     gene            17796..18740
                     /locus_tag="IXT20_002962"
     CDS             17796..18740
                     /locus_tag="IXT20_002962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HAQ4861321.1"
                     /translation="MDFFTNPDILLGDKLLEILYIVMGLVLIYTGVRNLTDKTNPHRY
                     GSAFFWTVLGVVIAGGRWLPAIVNGGLIFAMTIPAIAKKVSKGESRLPSKEYMEKMSD
                     KLGMKIFIPALSIGVFAILFALFTNLGALVGVGVGVFAAMLILMFFSRDNRPSTFLDD
                     AADMLGTVGPLSMLPMLLASLGAVFTSAGVGTVISNAVGTIVPEGNVNIGIIIYALGM
                     VIFTVIMGNAFAAITVMTVGIGAPFVFSHGANPALIGMVALTCGFCGTLLTPMAANFN
                     IVPVAMLEMKDKYGVIKNQLFIALFMLVFQIAYMILFK"
     gene            18939..19583
                     /gene="pcp"
                     /locus_tag="IXT20_002963"
     CDS             18939..19583
                     /gene="pcp"
                     /locus_tag="IXT20_002963"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HAQ4861322.1"
                     /translation="MKVLVTGFDPFGGDKVNPAYEAVKKMPDEISGAEIIKVEVPTVF
                     EKSSQVVKEAIQQHQPDVVICVGQAGGRSAVSFERVAINLAEARIPDNEGNQPFDTAL
                     EENGPAAYFTSLPIKAMTKNVQDHGLPAYISYTAGTFVCNDIMYRLLHLIETQFPAIR
                     GGFIHVPFSPDQVIDRPVGTPSMSLEDIASSLTYAVEAAVENKEDISGNAGTTH"
     gene            19662..20939
                     /locus_tag="IXT20_002964"
     CDS             19662..20939
                     /locus_tag="IXT20_002964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4861323.1"
                     /translation="MEEWQSVFEEWFPKEISKSYPIKISKQYTSSQRWEIYAKLTKKQ
                     RELVDKHRRYLISSRFMEEHYLAATDWVFSDFKINPFFRTKRSQQKLYCECGRELKVQ
                     YIVKSPKTGKILKLGINHFADHLHVSPTVAASIHQGMTKVDLALDELLWLKQKNIDFP
                     EGLWQKYCFVLYQNRRMKQPYLPDIKLAQRLAEFRQVEMPIYIADYQALENEIKKISE
                     HINGQSKKRQIKKELFDDFAEELVKDVEEFLINYRAFLRKDWQSIVYEEVPVHPNAYF
                     ETFISVLRKTKRQRTPEVTAQMEYFAKNQRFIQPKIYLFIWKQYCRYGFTEGFFDSIP
                     RIVRNGFLKVLRKEREAIQSADKKDRTVSKEKWQLVVKDIQSGNVQETIDKWKGKHYR
                     FTEAQKQALEYYQKLEESLRFNDEARKYLKELL"
BASE COUNT         6769 a         3786 c         4367 g         6062 t
ORIGIN      
        1 ctgttcttat tacttgatgc agggcagctt tttcacaacc ccttattcac ggtgttccag
       61 aagtagcttc ttcttatttc ttgaagctga tcacttctgg aacatcctct ttttttattt
      121 ggacggataa tggtcattcc atttctttga tcattttttt cattttgtcg atctcttctt
      181 cttgtgcctg gatgatttct tccgctaatt tttttacttc aggatctttt aaatcagctc
      241 gtttacttgt caggatagcg atagaatgat gaggaatcat ggccttcatc caagctgtgt
      301 cgttgacagt agtttggctt cgtaccataa acaaggaacc ggcaaaaaca agaagactag
      361 cggctaagat cataacattt atctttttgt tttcatacat tttccacatg aacaacagca
      421 tgatgactgc catgactgca cccatcatca acgccatgaa tactcgtgta cgactgaaga
      481 agatatgatc gagcgcatag acattcaagt acatcatacc aaacataact accgttgaaa
      541 caccgatcat caacaaaaac ttccaatact ttttcattta aaacaaactc ctttttttca
      601 ttcctttctt ctatcatgtc atgagcatgg agaacaatca attattttgc acagagggtt
      661 tttgaacacc gtttaccaag aggtcatgag actgtttatt cggaaaaagg agttgcaaca
      721 agacttttgt cacaactcct ttcttcaata gttccatcta ttccacataa ataaaataaa
      781 attttctatc gatttcattc attcgtcaat acgaacgcat cattcttttt ttgataactg
      841 aaataaatga acaggtaaag caggccagtc aatacagtta ccccaatgga gttcgtgaac
      901 aggataccaa ggaaaatcaa tcctaagaca gctaatgcaa aaaagctgat tcctttatcc
      961 cataatgttc cttttcttag tgccagtaca aaggatgccg cactgataat gatccagatc
     1021 aataatactg tatacgaaag ggaacctgcc agatagccaa ataatttgtc accgacaaaa
     1081 taagaaagag ctacaccaag ataaagaaca ctggtacaaa ataaaacagc acgttgcggt
     1141 acttgatgct tgtttaaaac agctagtttt ctagaggaac cttttcgatc cttcaaacgg
     1201 aagaacaaaa ttcttgaaga tgcatacaca cccgaattga tagatgagaa caaagcaagg
     1261 acaataacaa aattgacgat atccgctgca aatgggatat tcattttttc aaataccatc
     1321 acaaaaggac tgacattcgt gcctgctaaa gtattccaag gaaaaagaat caaaagcaaa
     1381 aacaaaggaa tgatgtaaaa ggaaatgatt cttcccatga cacctttgat ggctttagga
     1441 atcgcacgtt taggatcatc tgcttcgctt accgtgatag caatcagttc agaaccgccg
     1501 taagaataga taactacaag caaagaactg atgattccct tcataccatg aggagtaaac
     1561 ccaccatggt cagtcaattg ctgaagggta gggaaaacgc ctgtgcccaa catttgctga
     1621 ccgacaagat aagtagcaaa aatgattagt aagataatga cactgatttt agcaaaagcc
     1681 agccaatatt ctgtttctgc gaataaccgt accgaataaa gattgatgat cgtagttaaa
     1741 acagccaaca taaaaacaaa tacccaggct ggaatgtgcg ggaaccacaa ctgcaaaaag
     1801 gaagcagctg caacagcttc agcgatgata ttgataatcc acatagacca gtagacccaa
     1861 tcagtaaaat cagcagaatg agcacctaga taagggcgta tcaaaccaga taatccgtga
     1921 tgcgtgtcgc caccgcttaa aacgattttt cccagacctt tcatcacgat atataaaatc
     1981 agcccgccaa tcaaaaaagc aatcaataca gaagggccgg cgatcccaat cgcttcgcca
     2041 cttcctttaa atagacctgc accgattgct ccacctaaag ctaacatggt gatatgtctg
     2101 gagctaagtt gttttttcaa ttttttatct tccataaatg acctctttct tttaaattca
     2161 caatcaaaat ttatattaag aataaatagc aatatttaac aatgactaat gataacaaat
     2221 tttcagacaa ttacaactat tcagaaaact tttaggcgaa ttttaataga agaatgattt
     2281 atttcatcat attttcatac aaatgttaaa ttttcataca aatataggaa gcgttttact
     2341 ggctttttac tatttaaaca ggctatcata ggaaaaagtg acagagaaag tgaggcggaa
     2401 aagatgaaca aaaatgacgt actgctagtt gcaaaaaata aatcacttta ttttttgcct
     2461 tccatccgct ctttagatgt ttggtccgga aaagatttac tacagtcgga attcattaaa
     2521 tcagctagac aacagttggc aaattatcag ctgatcgttt ttttagatta tggatttaat
     2581 atggagatgg ctcgacgcgt gcgcccattt accaatgcaa aaattgtgtt atttttttgg
     2641 aatcacttca aagcagaaca cgaggcgatg ctgaagcagg cacaagatga accggcaatc
     2701 gatgagattt atcattttga ttttttggaa gcaaaagaac ttgggctgaa acataatagt
     2761 tcattttatt cgaaacatat ggatttttct tctagcgaac caaagacgga tctatttttt
     2821 ggtgcaacga ataatgggag aaaagaacga gcagaaagct ataagcagga atttataaaa
     2881 agagatgtta cgaccaacta tttcattctt ccacatcgag ggaatgaaca acctggatat
     2941 cttgcttatg aagagtacct gaaaaaaaca ggggaatcgc gcgggattct ggaactgttg
     3001 cgggagggtc agcaaggagt gactttgcgg acatttgaat ctatgtattt tcaaaagaaa
     3061 ctagtgacgg ataatcaagc gattgctcat tatcattttt atcgtcctga aaatattttt
     3121 ttgcttcgag aacgtgattt agatgaatta ccggaatttc tgcatacacc ttttaagcca
     3181 atcccatcag agctattgga attttatgat gcagaaaatt gggcaaaacg atttatcact
     3241 aacgattcca ctatttatga gcaatacgaa tatgacctct cctctgttga aaataaataa
     3301 aaaacctaca ttttgaagag attatgtacc gaaacaaaca agcacaattc ttatggcatc
     3361 atgagagttg tgttttcttt ttgatctttc attgggctta aaaaaagaga accagctgct
     3421 gattcagctg attctcatca gagagccttt tattctgcgc caaggattcc ttcttttgca
     3481 tggactaatt gggtcaaaaa ggcacaatat ggtgtagcaa tatcatattt ttgtcctttt
     3541 cgccaaacgg caccattgat gtagtcgatt tcagtcaaac gatggttttt gatcaggtct
     3601 tggtacatag atgggtaatg caacccgatg ttgtcaggat cataagtaga agcaatatgt
     3661 tggtacactt cttcttgatc taatatgatt ccttcttttg ctgcgataga agcaaattca
     3721 gatacgattg ttcgaaccat gctctctgcc gttttttgcg cgcctagttc agcaatatta
     3781 caatccaaaa tcgtgcataa accattcaat gttccattta cacaagcttt tcgccaaatg
     3841 gaataacgaa cattatggct atagatcgga tttaattttg cttcttgaaa gacttccaca
     3901 actttttttg taaatgcttc gccttctggt tccaagttct ctagttcgat ttctccatca
     3961 cctaacaatg tgacttttcc gggaccagct agaccagctg tccacatcgt gatcccaaat
     4021 aagatatttt tctttggcac atatttttcc agtacatctt catgacctaa tccatttagt
     4081 aaacacaaaa cataagtatt gtcagtaata atggattgga tcgaacgaaa catatcatct
     4141 aattgattcg cttttgttaa tgctacgatc aagtccacgt gttcgttgct ttcgatgatt
     4201 tcttctggtg aatagattgg cagttttgcg accacttctt taccgttaaa gtcagcaatc
     4261 aacccatttg tacgaatggc ttcaatgtgt gcaggccaac gatcgatcaa gaggacctca
     4321 tttccgcttt gatgtagcat caatccgatt cggctgccca ttgctccggc tccagctatt
     4381 gcgattttca tgttttttct ctcctttatc aatgagttcc cttttagtat atagaaaaat
     4441 ggaagaaaat agcattgttt tgtttgaaaa aaatgaaaaa aagtgtctta cttaagaaga
     4501 aagtgaggga tacagatgga tattttggcg gcaattgatt catttaaagg aagtgcttct
     4561 tcatttgaac taaatcaggc agttctaaat ggctgggaag atcgtaaagg aagaaaaatc
     4621 aatgtgccga ttgcagatgg cggagaaaat actgcagaag caatctatta cgcgctcggg
     4681 ggaacatggc gcaccattga tgggatggat cttttattcc gtcagcgaaa aatcagctat
     4741 ttattgacaa cttggcaagg aaaaaatacc gcagtcatcg aatctgcaca agcattgggg
     4801 ttagatctac ttgataaacc aacggatcaa actatccgac ttgcttccag ttatgcctta
     4861 ggagaactta tcaaagatgc actgcttcaa gaagtagaac aaattattgt cactttagga
     4921 ggaagcgggt gtagcgatgg tggtctagga ttactccaat ccttgggtgc ttctttagaa
     4981 gggtacacag aaggaaatcc tttattatct actaaaaaac ttcattttga tcgaagaaac
     5041 gttcttgttt ctaaagtaaa attgattatc gctgcagatg ttaccaatcc atatacaggt
     5101 caacaaggag ctgcttggca atttggaaga caaaaaggag gaatagatac gacgctagca
     5161 ttcttagata aacaagcagg aaagattgct cagcaaataa aagatttctg ggaggtggat
     5221 ttagatgata ttccaggaag tggagcagca ggcggtattg gtggagccct ttttttgttg
     5281 ggagcgaaga tggtttctgg tttcgattta gtcagccaaa tcactggatt ggaaaaaaag
     5341 atcgaacaag cagatgtcat cattacagga gaagggcgga tcgatcagca aacgatccac
     5401 ggaaaagttc cacacggtgt agcagtgctt gcaaaaaaat atcgaaaacc aataatcgct
     5461 atttgcggaa gtagagacca agagctagaa ggactctctg attttctccc aattgtctta
     5521 agtattcatt tgggtccaat aggtttagaa gaagcaatgg aacacaggca aacattagaa
     5581 aaaacaaaaa tcttaggaca gacacttagc agatttttca ccatcctccc gaagtagttg
     5641 acaaaatcat tttctttatg ctatcgtaat ttcaaatata ggacaaagtg agggatccgt
     5701 gcattgaaaa attaagtcga ttgatgaaag ctgcattcag actgtacctt gtggagacaa
     5761 ggaagcagtg tgcagatttt cgggattggc ttggtttttt cgatgtatgg gtctttttgt
     5821 gtgtatccaa cccttgcatc ctctttagcc agttctgaaa ataggactgg tcttttttta
     5881 cgaggtgatg gaaaatgtct aaaatcgaaa taaaaaatct gacattcggc tacgacagcc
     5941 aaggcacatt attatttgaa caagcaaatc taaattttga cacacaatgg aaactaggac
     6001 ttatcggacg aaacggtcga ggaaagacaa ctttactgaa tattctacaa aacaaactac
     6061 cttatcaagg gcaagtaatc catcagcaag aatttgccta tttcccgcaa cagacaaaag
     6121 ataaagaacg tttaacctat tacgtgttaa atgatattac ggattttgag atatgggaaa
     6181 tcgaaagaga gctccaattg atgcaaacag atcctgaaat cttatggaga gaattcagca
     6241 cactatcggg gggagagaag acaaaagtcc tactggcact tttatttgtg gatgacactc
     6301 atttcccgtt aatcgatgaa ccaacgaatc atttggatat ctctggtaga aaacaagtag
     6361 cggcttattt gaaaaagaaa aaacaaggct tcatcgtggt cagccatgac cggggattta
     6421 tcgatgaagt agtggaccat gttttagcaa tcgaaaaaag tcaactggaa ctttatcaag
     6481 ggaatttctc tatctatgaa gaacagaaaa aacttcgtga tgaatttgaa atggctcaaa
     6541 atgaaaaatt gaaaaaagaa gtcagtaggc taaagaaaac agcagctgaa aaagccgaat
     6601 ggtctcgttc ccgagaagga gataaaacaa agaaacaagt cggattcatc gatactgaat
     6661 ctagacgagt gaataaagga gcagtgggtg ctgatgctgc acggacgatg aaacgatcca
     6721 aagcaatcgt gaatcggatg gagacccaga tcagcgagaa agaaaaacta ttaaaagata
     6781 tcgaatatat cgattctttg acgatgaata gccaagcgtc tcaccataag cgacttttaa
     6841 gcgtagaaga tcttcaatta gggtatgaaa atctgttatt cgagccaatt cattttacaa
     6901 tcgagcctca tcagcgggtg gcgatttcag gtcctaacgg tgcaggaaag tcatccatta
     6961 tccattatct tctgggggca ttcaacggca aggttatagg agaaaaatcc cagccaaaac
     7021 atctgagcat tagttatgta cgccaaaatt atgaagacaa tcgaggaacg ttggcggaat
     7081 ttgcagagaa aaaccaagta gactaccaag catttttgaa caacctccga aagcttggga
     7141 tggaaagaga tgtttttcat aacaagatcg agcagatgag tatgggccaa cggaaaaaag
     7201 tggaattggc taaatcttta tcacagccag ctgaactata tacatgggat gaaccattga
     7261 attatttgga tgtcttcaat caagaacaat tagaacaact gatcttgaac gtgaaacctg
     7321 ccatgttact agtggaacat gatcaaacct tcctggataa agtatctact gagattattt
     7381 ctcttgagag aatctaaccc ttgtaatctc aagaacaaca aggtatggtt aaggggaact
     7441 ttgccaacga aggagagaat cacatgcttt atgtaacgag acacggagaa acaacttgga
     7501 acgcacaagg attagtttgc ggacgggcag atgtgccgct gactgaaaaa ggacagatgc
     7561 aggcacagaa actagcagaa aaggtagtag atttgcctgt tccaatcacg aaaatcatcc
     7621 attcccctct tcaacgagca agagatacag cacaagcagt tgctgatcgt ctcagcttgc
     7681 cgctgaccgt tgatgaacga ttggtagaaa tggattttgg ggattatgat ggactgccaa
     7741 gcaaagatga gaactttcaa aaagcacgtt tagcgttcgc tgtacgattc ccaaatgggg
     7801 agtcagtctt agatgtgtat gcacggatcg ttcctttatt gaaagaatgc atcgaagacg
     7861 aagaaaatgt ctatctgtta gtttgccata atgccttgat aagagtgatc aatgcttatt
     7921 ttcatccgat gcctaatgag gggttcttta cattcatggt agataataca gaattggttt
     7981 cttatgagta attatgtttt tgtgttattt aaataaaata tttcaaggca ctgcttttgc
     8041 ttagcagtgc cttttttagg ttatgacaat tcttttgata atataaactc aacctttaga
     8101 aatgaaattt aattatttta ataaaagtat tgaaattttg tttctttaga tatatactct
     8161 agtcatagaa atgaagacgg atacagaaaa agagggataa tatgtttggc ttttcaatat
     8221 ttatgaacga ttctttgtca gaagagaaga aacagtatat ccgccaaatg gcatccaatg
     8281 gctttgtcgg tattttcact tccatgcata taccagaaga tgatgtttcg gcatatcaaa
     8341 agcgattgat cgaactagga agcgaagcga aaaagaataa actagaattg atggtcgata
     8401 tttcaggaga ggcattggaa cgagcgggtt tttcattaga agatctaacg cctttgaaag
     8461 caattggtgt aacaggttta agaatggatt atcatatttc caatcagcag attgccgact
     8521 ggtcacatca attgaaaatc agtttgaatg ctagcacgat cacgcctaaa gatatcgatg
     8581 aattgaaaga agcagaagct gatttctcac agattgaagc ttggcataat tattatccac
     8641 gtcccgaaac agggctggac aaagaatggt atcagaaaaa aaatcaatgg ctcaaaattc
     8701 aaggtctcct tgtccaaggt tttgtaccag gagatacaga gttgagaggc ccactatatc
     8761 aaggattgcc aactttggaa gaacatcgag ggatccatcc tcttgcaggc gctctagatc
     8821 tattagcaag taacacggat attgtttaca tcggagatgc aggtctgtca gaaaacgttc
     8881 aggagcaatt tgcttctttc caaaaagaac agacagtctt gctccatacg gaacctgtag
     8941 atgaagaatt ttacgaatat atattaggga agcatacgaa tcggcaagac gatgcaagag
     9001 atgtcatacg aagcgcagat gcacgttttc gagagattcc gcctatccca gcaagaaata
     9061 cagctacgcg tatgaaaggg tctattactt tagataacga aaagtatttg cgctatatgg
     9121 gtgagatcca attaaccaaa tatgatttgc cagcggatga aaaagtgaat gtagtagcta
     9181 aagttatcaa agaagagcta ccattgatca accaaatcaa agctggtatg aactatcaat
     9241 ttataagaaa agaaggaaga taaaattgag caaaatcaat ttagagaacc taacaacgga
     9301 acgcagaaac caagaaactt ttggattaga tgagatgagt atttctcaag ccgttcaact
     9361 aatgaacaaa gaagatcaga aagtggccgc tgcggttgaa aaagaactga caagtatcga
     9421 accagtgatt gctaacacga ttgagtcctt taaaaaaggt ggacgtctga tttatatggg
     9481 agccggaaca agtggaagac taggtgtgtt agatgctgct gaatgcgtac cgacattcgg
     9541 tgtagaaccg gaaatggtca taggtctaat tgctggtgga gaagaagcaa tgactgtagc
     9601 tgtcgaaggg gccgaagatt cttatgagct agggcaagaa gatctaatca agctgaattt
     9661 gacaaaaaat gatatagtta ttggtatcgc agctagcggt cgtacaccat atgtgatcgg
     9721 tggattagat tacgcaaaat cgatcggtgc tgtcacagga acgatttctt gcaataaaca
     9781 agccgaaatc agtaaacacg cggatttccc aatcgaagtc gatgcaggtc cagaattttt
     9841 gacaggctct actcgattga agtcaggaac agcacaaaaa cttatcttga atatgatttc
     9901 tactatcagt atgattggta taggtaaagt attcaataac ttgatggtcg atgtcaaacc
     9961 aacaaatgaa aaactaatcg aacgctcaaa acgaatcatc atgcaagcga cagaagctga
    10021 ttacgaaaca gctgaaaagt actttgaaga agcagatcaa aatgttaagc tggctatcgt
    10081 catgattttg acaaatagtg ataaagagga agctcaagca aaattagctg aggcaaacgg
    10141 atttgtaaaa gggaccttat gagggggaaa agaccatggc aaaaaacgaa ctgactttgg
    10201 aagaactggc aaaacaaatt tataaagggg caggcggcat gggcaatgta gaaagcattg
    10261 tccactgcat gactcgtgtg cgtatgaaga tcatcaatga tgatcttgta aatgtctctg
    10321 aattaaaagc gattccagga gtgcttggtg tagtagaaga tgaacagctt caagtcatca
    10381 tcggacctgg gaaagtgaac aaagtagcac agcaaatggt tgatttggca ggcgtgaaat
    10441 taggagaaaa actttcttct gtaagtgcag ctacagcaaa ttcatcagca tctggaaaag
    10501 accgtgtaaa cgaacgggca caagaaatga aagcggcaca aaaatcgaaa caaaaaccat
    10561 ctaaaatcaa atcggtatta aaagacattt ctaatatctt tgtgccaatg attcctgcat
    10621 ttgtcggtac tgggatcgtg gcagggattg ccgcagtttt agcaaactta gtcacagccg
    10681 gaacacttga tgcagcaaca tggcaacaat atatcgatgt gatgaacatc ttgaaaaacg
    10741 ggatgtttag ctacctagtt atctatacag gtatcaatgc tgcacaagtt tttggtgcaa
    10801 caccaactct tggtggagtt atcggggccg ttgtgatgct gacagggatg aatccggaag
    10861 caccactgaa aaacttattt actggtgaag ctcttgcagc tggacaaggt gggatcctag
    10921 gtgtcatctt cgctgtttgg ctattgtcaa tcgttgagaa aaaactgcat aaagtagtac
    10981 ctgatgcggt agatatcatc gttacaccaa ctctttcatt gattgccatc ggtctgattg
    11041 aaattttctt catcatgcct ttagcaggtt tcatttctga tggcatggtc ggtgggatca
    11101 actgggtact tggcgttggt ggagcatttg ccggcttcgt tctaggcaca ttattcttgc
    11161 cgatggttat gttcggactg catcaaattt tgactccaat ccatgtgcaa atgattgatg
    11221 aaactggtcg tacgctattg cttccaattc ttgcgatggc tggtgcagga caagtgggtg
    11281 cagcattggc attatggatt cgctgcaaaa aagataaaga attaacagaa atgatcaaag
    11341 gagctttacc agtcgggatc ctaggaatcg gcgaaccatt gatttacgga gtaacattac
    11401 ctctaggacg cccatttatt actgcttgta tcggtggagg tatcggcggg gcagtgatcg
    11461 gaatgatcgg aaatgtcggg gctatcgcta ttggaccttc aggagccgct ttgatcccat
    11521 tgatctctga cggtaaatgg ttaggctaca tccttggatt attggcagct tatgctggtg
    11581 gcttcgttgc tacattcttc ttcggtattc ctaaagaccg tttagaaaaa gaagaattag
    11641 cgactgaaac agccacagta aatgaagtgc ctgaaaaagt agtacctcaa acttcagctg
    11701 aaagtgtgac tatcctctca tcagttgctg acggaacagt tgtttcttta gataaagcaa
    11761 gtgatccagt ttttgcacaa aagatgatgg gtgaaggcta ctttgtagaa ccagaaaacg
    11821 gacatatcta ttctccagtt actggggtag tcagctctgt cttcccaacg aaacacgcta
    11881 ttgggatcac aacgccaaat ggtttagaaa tcttgcttca tatgggaatc aacacagttg
    11941 acttaggcgg tacgccattt gatgtgaaag taacagaagg gcagcaagtg acacctgata
    12001 cgcttgtagc agaagctgac ttagaagcga tcaaagcagc aggcaaagaa acatccatga
    12061 tcgtcttggt aaccaatatg gatcgtgtaa aaaactttgt cttggaaaaa acaggtaaag
    12121 caaaagcaaa agcacctgtg atggatgtca aagcttaata tgatagaaac aatagaaacg
    12181 aaagaagaaa ctgaaatcta tttttcagct tcttcttttt tattatttat tgttttctgc
    12241 taaaatctga taaattcgtg tcacatcaga tggtgttccc cgtaattcat aatctgccaa
    12301 gaaaggaaag ccaaatgttt gtgcatattg tttggctgtt agacaatact gattattgaa
    12361 gtttttattt ccgctaccga ctacaccaag acaaagttta tgattgtctt catgttccag
    12421 atattcacgc atcgtttcgg tcaagatttc tgtatcaccg ttatctaaac catttccgcc
    12481 gtctaaataa gtaggaacga aagtaaaaaa tggttctgtt tctttcgtaa aatcactatt
    12541 ttcatggatt tcttttaaag taacttcagg attgacagga tcttctgcat gcattttttc
    12601 agcatattct gctagatgtt tggcaaaggc acgcgtattt cctgaaatcg atatatataa
    12661 aatgttcatg gatcatcctc caattctttc cttttagtat agcatctttt ttaagttttt
    12721 tggtggtgta atttggaaag ggactaggcg ctttgcttgt ataatgagaa aaaagccggt
    12781 agaattatat ttctattaaa gtttttatta attaagtaaa aataaaaaag gtttgttata
    12841 ctttttgata aaagcggttt tattttggag gatgcttaca tggatacaga gcaacagaaa
    12901 aaaggaattg gtttaattcc acttgctgcc ttagtaattg gttcagcgat tggaggtggc
    12961 gtatttggta ttatgacgga catctccgga tcggctggcg gcccagctat tttcagttgg
    13021 gtactcgtag gaacagcaat gttgatgctc tccttgtcga tcaataatat caataaaaaa
    13081 cgtccagatt taaaaggcgg gatctttagt tacgcagaag aaggttttgg ccgtttcgct
    13141 ggttttatca gcggttgggg ttactggctc acatgctggt taggcaacgt agcttttgca
    13201 acattgttga tgagcgcatt aggatatttc ttcccagtat ttgaaggtgg gcaaaatctg
    13261 gcatcgatcg tgatgagtac agtcttttta tggggatatg catggctagt gaatcgggga
    13321 gtcgaaaatg ctagtttcgt caatgctatc gtcacattct gtaaattagt gccattattt
    13381 ttatttattg tagtagctat cagcacgttt tcgatccaac aattcattga taacttttct
    13441 agcgacatta tattgaatgc gaatggacag ccgatcccgt tttccagtca agtattgagt
    13501 tgcatcatgg tgatgctttg ggtatttgtt ggtattgaag gagcctctgt cgtaggttca
    13561 cgagcaaaaa aacaatcaga tgttgggaaa gcaacgattc tagggatatt cggcttgatt
    13621 attatttacg tcttggtttc catgcttcca tatgggacaa tgacgatgaa tcagctgcaa
    13681 acgatcaagg cgcagcctgc tatgggctat gtgtttgaaa tgatggttgg taaatgggga
    13741 gcagtcgtga tcaacggcgg tttgatcatt tctttagttg gtgtttggct ctcatggacg
    13801 atcttaccga ttgaaacaat gggaaacatg gctgaagacg gacttttgcc taaagcatgg
    13861 ggaaaactca acaaaaatgg ggcaccaact tatgctatcg tattgacgac gttatgtacg
    13921 aatgtctttt tactttcatt attattcact gacagtgctt ataactttgc ttacacactt
    13981 ggtacagcag ctattttctt cacgtggctt ttcttaggat tgtaccaaat gaaactttct
    14041 tatcagcgga aagaatgggt ccaattttta gtagggcttt tagcaagtgc ctttcaagta
    14101 tgggctatgc tagtcgtagc ctttaatgaa gtaatgcttg ttcttgttct tttcttgcca
    14161 ggcatttatt tttacctcaa agcacgtaag gagcaaaacg agcctgtaca acatatccac
    14221 ttagataaga ttttacttat aggtgtgacg atggtcggag tgattgcaat ggttttattt
    14281 gccactggga tgcttcacgt atagaagtat aaaagaagta taaagagagg ttgtgagcct
    14341 gtcgtttatt caaaaacata ctaggaaagg agctgtgaca caaatgttgt cacagctcct
    14401 ttttcagaat aaacggtgtt taagagacag ccaatcggca ataaggctga acgtctcttt
    14461 cacagcctct tttttgatga gtcatcatac attttttcgc ttaaatacga gatatcctaa
    14521 tgctaagaac aaaacgatgt aagctaaatt accgaatagc atttgattag tagaaagatg
    14581 agtcaattgc ttcatgatct cttcgtttcc aatttggttt tgcagattca acatactgat
    14641 cggattccat ttgatccact cccatttttg gatgagtgca ataaggatac cagatatgat
    14701 tgacgaagca aaataaaaga cgattcctgc tgagatggat gcaccggaac tattgataaa
    14761 gcaagcaatc atcaacacta aacttaaaat cagccataaa ccgatataac tacccaaagt
    14821 ataagtgaac aagctttgga tcagtgtttg accagtagaa accttctccg tgaaggaaat
    14881 cgaagggaaa agaatcagtt tcatcaacac agtgacaata atagctaaaa ggtataaata
    14941 aacagaataa ataactaatg tgatccattt gcttactaat atttctcctc gagaatactt
    15001 tcgataaagg agatttttca atgtaccata ttgtgcttcc atcgaaatga tcgtgcttgc
    15061 agctgcaatc atgataaaga caatccaaga tgtggcacta aaaagctgag cagtcatttc
    15121 aactggatca atgatcgtcg tgtatttttt ggctagaagt gctgttccta tgagcaaaac
    15181 aactaataga acagataaaa cagcagttga ctttttcttg atcaatttaa ataattcttg
    15241 ttttacaagc aatgtcattt ttcttcttcc ccctttactg aatccagaat ttccaataga
    15301 gaagattcca aatcattcgt cagatgctgg acgtctgtca catggatatt ttgtgcagac
    15361 aatagctgga tcacggttcc taataattct tcgttttctt ttaaaagctt gattttatca
    15421 ggatcttcca aagtatatcc agcttctgtc aagatcgttc gagcagctag atcatttgtt
    15481 gtagagataa cgatgaaatc ttgcgctgtt tggttcaatt cagccattgt acattgtttg
    15541 atgatctttc cttgattgat caggatcagc tcatcgacca ttttttcaag ctcgctgagt
    15601 aagtgactgg agatcaagaa agaggtgccc tgagtagcca agtcggtaat gatttttcgc
    15661 acatctcttg ttgcttgtgg atccaaaccg ttcatcggct catctaaaat aacgatagac
    15721 ggatggttca gtaaggcaag agcaattccc aatttttgtt tcattcccaa cgagtaggat
    15781 ttcgcttttt tgtgaatgta cttttccatc ttgagctgat tcacgatagt gtcgatagca
    15841 gatacatcgt tggtttcaga gaataacttc agatgatcat agcctgttaa gaatggatag
    15901 atacccgggt attcgatcaa tgctcctact tgttccaacc ctttatgact agagggagaa
    15961 atctcatttt caccaataca gattgttcca tctgagtaat ggatcagtcc taaaatagct
    16021 ttcatgatcg ttgtttttcc agcgccatta ggacctacta atccaacgat tttgccaggg
    16081 gataagctaa aactgacatc gctgagggct tgtttgtgac caaagttctt ggataattct
    16141 tggaccgaaa gacttttcaa aaatattcac tccttttcta tagatagtac aagcatacac
    16201 ctgatgagtg gagaagtcgc taggtcattg gacttatttc ttattttttc ataaaaaaat
    16261 tgaatattaa ataaaaaggc ttattttttc tgatcagtac ttattccaag tgtctagtgg
    16321 ccgctagtaa tcttgatgcc aatgatactg atgagtagca tccccacaaa gaaccaagtg
    16381 gaaagattca gcttatcatg aaacaagacc acaccaatta tgaccgaacc aactgcgcca
    16441 atccctgtcc agataggata agagagactc atcggcaaac ttttgattgc ctttgacaaa
    16501 aagtaaaaac tagcaatcat gccaatcact gtgtaaaaag aataattgat tttagaaaaa
    16561 ccatcactcc atttcatcat cgttgaccag aatacttcca aaattcctgc tatgattaat
    16621 tccatccatg ccatacttac attcctcctt aactataaaa aaatacacaa aaagaggaaa
    16681 atcatccctt aaaagaccta atggaatgat catcctcttt atcctacccg gtggcagatc
    16741 gtatatcttt ttctgtagta tagcacacct atccagatat cggcaatctc atacttttta
    16801 gtaaagtgta ccaagcactg acggatatat cgttagcgga aaagaagatg aatgctttgc
    16861 aatttcatag aaagaagttg ttatttatta atcatttaaa aaaaacagta actagacctt
    16921 atttctatat attatcgggc aataaattaa aaaaagcttt accttacaat aagtaagata
    16981 atttttgtga taaaaaaata acgaaaaata tttaaaaata gtgtctattt cgtgagaaaa
    17041 caactatatt ttttatgtgt tttgctttat aattaaaaaa atattgtcat tttatggagg
    17101 aaaatatatg gaaaattatc ttagcctttt aggcgtcttg attgttattc tcgggtttgc
    17161 atttaaatta gactcgatat tgatcgtaat ggttgcttta gttgttacag ctttgactgg
    17221 cggtcttggc attcaaggaa tgttagaact tttaggtaca agttttgtaa acaatcgcgg
    17281 gatggcgatt tttatcatca ttatgttggc aacaggaacg cttgaaagaa atggtctgaa
    17341 agaatcagcg gcttccttga tcaagcgatt caagaaagta tctgctggta tgattgtaga
    17401 tatttatggg atctttcgaa tgatctttgc ggcttttaac gtgagttttg gtggagttgc
    17461 tggatttgtt cgtccaatca ttttaccgat ggcattgggg accatcgaat caaaagggtt
    17521 agaagtccat cctaagcatg aagaagaact aaaaggtatg gcttcagcta tggaaaatgt
    17581 ctgctggttc tttggccaag tactattcat cggaggagca ggggcgttgc tagtccaatc
    17641 aacactgaaa gatctaggat atgacgtaac attgggacag cttgctttag tggaagttcc
    17701 agtggctatc acggctttag ttgtggcaag tgtctacttc tatcttaaag aaaagaaatt
    17761 agcgaagaaa tattatccag gaaaggggca ataagatgga cttttttaca aatcctgata
    17821 ttttattagg cgataaatta ctggagatcc tttatatcgt gatgggactc gtattgattt
    17881 acactggagt aagaaattta acagataaaa ccaatcctca tcgttatggg tcagcctttt
    17941 tctggacggt cttaggggta gttattgcag gaggaagatg gcttccggca atcgtgaacg
    18001 gtggcttgat ctttgcgatg accataccag caattgctaa gaaagtcagc aaaggagaaa
    18061 gccggcttcc atcaaaagaa tatatggaaa aaatgtcaga caaactagga atgaaaattt
    18121 ttattccagc tttgagtatc ggtgtctttg cgatcttgtt tgctctattt accaatttag
    18181 gtgctttagt cggcgtgggc gtcggtgttt ttgcagccat gctaatcttg atgttctttt
    18241 ctcgagataa tcgaccaagt acatttttag atgatgcagc agatatgctg ggcacagtag
    18301 ggccgttaag catgttgcca atgcttttag cttctctagg tgcggtattt acaagcgctg
    18361 gagttgggac agtgatttcc aatgcagtag gtacgatcgt tccagaagga aatgtgaaca
    18421 tcggcatcat catttacgct cttggcatgg tgatatttac ggtaatcatg gggaatgcat
    18481 ttgcggccat caccgtgatg acggttggga tcggtgcacc attcgtattt agtcatggag
    18541 cgaatccagc gttaatagga atggttgcat tgacttgcgg tttttgtggg accctgctga
    18601 caccgatggc tgctaacttc aatatcgtgc ctgtagcgat gctggagatg aaagacaaat
    18661 acggagtgat caaaaaccag ttgtttattg cgttatttat gctagttttc caaattgctt
    18721 atatgattct atttaaataa tagaagagag gggttgtgac agaataattg tcgcaacctc
    18781 ttctaacatt gtagtggaac actatttttt tataagaata tattaagttt gatctgttat
    18841 gttatttaaa ctattgtttc ctttatttac gctgttcaag gaaaatggta tgataaaaga
    18901 taaccatatg ttaaatcaat cggacggagg aacaacagat gaaagtacta gtaacaggtt
    18961 ttgatccatt tggtggggac aaagtgaatc cagcctatga agcagtaaaa aaaatgccag
    19021 atgaaatcag cggtgctgaa atcatcaaag tggaagtacc gaccgtattc gaaaaaagca
    19081 gtcaagtagt caaagaagcc atccaacagc accaaccaga tgtcgtgatc tgtgtcggac
    19141 aagcaggtgg gcggtcagca gtctcgttcg aacgtgtcgc aatcaatcta gcagaagcaa
    19201 gaatccctga taatgaagga aatcagccat tcgatacggc tttagaagaa aacggtccag
    19261 ccgcttactt tacctcattg ccaatcaaag caatgacaaa aaatgtacaa gatcatggtc
    19321 taccagcgta catctcttat acagcaggaa catttgtctg caatgatatc atgtatcgtt
    19381 tgcttcattt gatcgaaaca caattcccgg caattcgagg tgggttcatc cacgtgccat
    19441 tctcacccga tcaagtgatc gatcgtccag tgggtacgcc ttctatgtct ttagaagaca
    19501 ttgcttcttc tttgacttat gcagttgaag cagcagtaga aaataaagaa gatatttccg
    19561 gaaatgccgg aacaactcac taatttagca agaaacactg gctggttgct aacgttatgt
    19621 tagtagccag ctttttgtaa ttagaaagga aagaaacagc cgtggaagaa tggcaatccg
    19681 tctttgaaga atggtttcca aaagaaatca gcaaaagcta tcccatcaaa atttctaaac
    19741 aatacacaag cagtcaacgt tgggagatct atgcaaaatt aacgaaaaaa cagcgggaac
    19801 tagtcgataa gcaccgccga tatctaatca gctctcgttt tatggaagaa cattatttag
    19861 ctgcaacaga ttgggtgttt tctgacttca agatcaatcc gttttttcga acaaaacgaa
    19921 gccagcaaaa attgtattgt gagtgtggta gagaattaaa agtgcaatac atcgttaaat
    19981 cgccaaaaac cggaaagatc ctaaaactag gcatcaacca ctttgccgat cacctgcatg
    20041 tctctccgac agtggcagct tcgatccatc agggaatgac aaaagttgat ctggccttag
    20101 acgaattatt atggcttaag cagaagaata tcgattttcc tgaaggacta tggcaaaaat
    20161 actgttttgt cttgtaccaa aatcgtcgga tgaaacagcc atatttaccg gatataaaat
    20221 tagctcagcg gctcgcagaa tttcgccaag tagaaatgcc gatttacata gcggattatc
    20281 aagcattgga aaatgaaatc aagaaaataa gtgaacacat caatgggcag tcaaaaaagc
    20341 gacagataaa aaaagaacta tttgacgact ttgcagaaga attagtaaag gatgtagaag
    20401 aattcttgat caactatcgc gcatttttac gtaaagattg gcaatccatc gtatatgaag
    20461 aagtgccagt acatcctaat gcttattttg aaacatttat ttccgtgttg agaaagacaa
    20521 aacgtcaacg aacaccagaa gtcactgctc agatggaata ttttgcgaaa aaccaacgat
    20581 tcatccaacc aaaaatctat ctatttattt ggaagcaata ttgtcgctat ggttttacag
    20641 aagggttctt cgacagcatt cctcggatag tcagaaatgg ttttttaaaa gtattgcgta
    20701 aagaacgaga agctatacaa tctgctgata agaaagaccg tacagtctca aaagaaaaat
    20761 ggcaactagt agtgaaagat atccaatctg gaaatgtgca agagacaatc gacaaatgga
    20821 aaggaaaaca ttatcgtttt actgaagctc agaaacaagc gttggaatat tatcagaagt
    20881 tagaagaatc actgcgtttc aatgatgagg caagaaagta tctgaaggaa ttactataaa
    20941 aaatagaggc tgggacagaa gcgtttaact ccaagaaata agaa
//
LOCUS       DACGLE010000213        20862 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0534
            SAMEA3304449-rid9560453.guided.045, whole genome shotgun sequence.
ACCESSION   DACGLE010000213 DACGLE010000000
VERSION     DACGLE010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304449
            Sequence Read Archive: ERR985064
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20862)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20862)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702225.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702225.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 20:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,045
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 27 of 67
            Pseudo Genes (incomplete)         :: 25 of 67
            Pseudo Genes (internal stop)      :: 19 of 67
            Pseudo Genes (multiple problems)  :: 4 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20862
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304449-rid9560453.guided.045"
                     /strain="VREN0534"
                     /db_xref="taxon:1352"
     gene            267..1304
                     /locus_tag="IXT20_002965"
     CDS             267..1304
                     /locus_tag="IXT20_002965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HAQ4861324.1"
                     /translation="MSEKELMKVVGFYEGLPIDDPKSFIDEKEQIPVPSSRDLLVKVN
                     AVSVNPVDTKLRQDNGIRNALRILGFDGVGKVVAVGDEVQKFSVGDRVFYAGTTTRAG
                     SNQEYQLVDERIVALAPKNLSDEEAAALPLTSLTAYELLFEKFGLTPEENANRGKKIL
                     VINGSGGVGSILNQLAHWAGLEVYATASPKNFEWLKKTGVDYPIDYHHDLSKSFRELG
                     IDQVEYVAVLFDITRYFDQIKHLVRPFGHVGTIVGIKEPLDIGVWKNQSISFDWEYMF
                     AKTDYNYEIETQGEALAHIAALANEGIIRSTLSKVYSDGMNAKNLKQATKDVETGHML
                     GKVVVSGPFNG"
     gene            complement(1393..1716)
                     /locus_tag="IXT20_002966"
     CDS             complement(1393..1716)
                     /locus_tag="IXT20_002966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HAQ4861325.1"
                     /translation="MPSFEFTLLTILGCTLATWLSRVLPFVLLKKFDLPQPLLEYLSF
                     VPIVIMSALWFSSLFTQNIGHLPQINLENALASVPTLLAAILSKSLLVIVLAGILSLS
                     LIRLL"
     gene            complement(1703..2410)
                     /locus_tag="IXT20_002967"
     CDS             complement(1703..2410)
                     /locus_tag="IXT20_002967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HAQ4861326.1"
                     /translation="MDEKLDIKTAIKDTLPTVFGYIGIGLAFGIVGKAAGFHPLVVTL
                     MSLLVYAGSAQFITVSMLASHSPLLSIVFSTFLVNSRMILMSMTIAPYFKKNRLLQNL
                     LIGTLLTDESFALGMNKLNYTGQKLNFRWMNTANWISYLTWVGSSLVGALLGNFITDP
                     KKFGLDFAIVAMFIGLLYLQVISDRNTSKRLQLILIGLTLILVYVGLIFIPSNLVIVV
                     VTLIGCGLGVWIKHAFF"
     gene            2496..2837
                     /locus_tag="IXT20_002968"
     CDS             2496..2837
                     /locus_tag="IXT20_002968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylulose kinase"
                     /protein_id="HAQ4861327.1"
                     /translation="MVGLKRKELAGRRFTRIVSVGGGAKNLAWLQIQADIFDATVYCL
                     SMEEGPALGATMIAALGLGWFDTPESCCQNFIQYTDAAEPLLQNVEKYHKIYQQCRKI
                     YPATKEICHDL"
     gene            complement(3008..3856)
                     /locus_tag="IXT20_002969"
     CDS             complement(3008..3856)
                     /locus_tag="IXT20_002969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4861328.1"
                     /translation="MNNWLEYFPENVLERGYSYHLHGFVRHLNYTSKYLSATVSGTED
                     YKVVITWDEKTNMTCDCLYAIEGKKCKHMAAVLFAYEERPIKKSNYSLSELSSLVSSA
                     SSSLVRELLTEILIEHPHFIERFKVKMPFHAINYSDKLTTIIHKYDHIIKKNKNRKTA
                     KFIMEMRKFIQEAVESLIQQNAYLPAFELINEVIATLETFYWEPEDERTLLLIEDCYY
                     LWKELLAEAPHAEKRQMFSWFVCQVDHTDASYSKRYSIKILKEDFREKEFSNQKKKID
                     KKLKKR"
     gene            4454..5317
                     /locus_tag="IXT20_002970"
     CDS             4454..5317
                     /locus_tag="IXT20_002970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAQ4861329.1"
                     /translation="MLKDLNQLIDYIEEHLTEGLSYKIIAENTGISEYQLKRIFTFIA
                     GMSLMDYIKNRRLALANRDLLDGSRVTETAFRYGYETVEGFSRAFRDWSGYLPSEISR
                     YGIQKSFPRLTFFIDVRGGKSMEFRIEKKEQFYVAGVAKKVPVKFEGVNQEIQKLAQT
                     ITETQREEMHKLGDLYPNQVINVSYGFDGDRLEEKGSLMHMIGFATTKENPYADLEML
                     AIEEHTWAIFPNKGPFPQTLQDTWGKIYAEWLPSSDYGLVEAPEISFTKWGEDFSNVY
                     SEIWIAVKAKK"
     gene            5417..5899
                     /locus_tag="IXT20_002971"
     CDS             5417..5899
                     /locus_tag="IXT20_002971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAQ4861330.1"
                     /translation="MAKYRIEEKPDFTVTGIGVELKSSNTDFVGLAAEKQAFEQKVIG
                     NGTIDALKQAAENHYFFTVNEPYNGKMMYYVGVQTSQDVPNTTRIIPFPKGTYLVVSA
                     LAESPEELRNDLTNTAFGHVLREVEDYAYVGGPNAVVEMGVTNGKYMGEIWIPVVNKP
                     "
     gene            complement(6162..6629)
                     /locus_tag="IXT20_002972"
     CDS             complement(6162..6629)
                     /locus_tag="IXT20_002972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HAQ4861331.1"
                     /translation="MKYSLQFSDAIHVLAYIEMFQDTRSLSSEMIASSIETNAANVRK
                     IMSQLKKSGLIFTKTGKPQPSLAKSPDQISLLDVYRSIEGNTQLIQVDPKTNPNCVVG
                     ANIQEALEESYARLQKKAEEEMAKITLDSLIHKIAVLEKEKRPENTGLVERFL"
     gene            6752..7603
                     /locus_tag="IXT20_002973"
     CDS             6752..7603
                     /locus_tag="IXT20_002973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAQ4861332.1"
                     /translation="MHYLVTGATGGFGGYALEFLKKMVPQNEIYVLVRSEEKGEALKE
                     AGFNIRIGDYDDLDSMKEALKGIDRLLFVSGVPGNRQAEHKNVVKAAQAAGVSYIAYT
                     SFADADHSISVLAPDHQFTEKMIKDSGISHTFLRNNWYVENELPIIGQALKTGQFVYA
                     AGNGKTGWALKREYAEAAAKVLVESDSPEILELSGNLTDYEELAKALERATGKELEII
                     EASDAAFVENLKEAGFPQEAADMFLAIQHDIKKDQLAISSDDLEKVLGKPLTSLEAAL
                     KEILDQE"
     gene            complement(7854..9812)
                     /locus_tag="IXT20_002974"
     CDS             complement(7854..9812)
                     /locus_tag="IXT20_002974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ4861333.1"
                     /translation="MKAYLQRMGRSLQLPVAVLPAAALLVGIGNWWASYSNDIVAHFL
                     QAGGNAVLGQLPLLFAVGLALGMSVDKSGAAALAGLVAFEVPVNVLKTDSVATLLNIK
                     PESVDPSFAQISNVFIGIIAGLIAAALYNRFHEAKLPMALSFFSGKRLVPILAAFVML
                     LISAVLLLVWPPVYSALVSFAKFILSLGWGGAGLYGFFNRLLIPTGLHQAMNSVFWFD
                     VAGINDIGKFLASEGTKGVTGMYQAGFFPIMMFGLPAGAYAIYRNARPENKAKTASLM
                     LAAAFASFFTGVTEPLEFSFMFVAWPLYVLHAIFTGISLAVSAFFHWTAGFAFSAGFV
                     DYFLSLKNPIANQPLMLIVQGLVTAVIYYFGFNFAIKKFHLMTPGREEADLSDDDTAT
                     TNSDNKYAAQASKIYAALGGADNVTSIDNCTTRLRLQVKDTGLIDQNKIKATGVPGMK
                     IIDGKNAQVIVGTEVQFVADEMAKLHGGAAARPAQTNTVVKTETFAGETIEQDIYAIA
                     NGKLIPITEVSDDVFAEKMMGDGYAVLPENGEIFSPIAGTITNIFPTKHAVGIQTDAG
                     IEVLLHMGINTVDLKGEPFTLYVEEGQKVARGQLIALVDLAAIQSAGKNTDMVVVFTN
                     GDKVQSLEIEPARDVKANDKIGSVSNKA"
     gene            complement(9809..10681)
                     /locus_tag="IXT20_002975"
     CDS             complement(9809..10681)
                     /locus_tag="IXT20_002975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ4861334.1"
                     /translation="MKIKKIFNQNAVLVDDGGQEKVAIGKGIGFDRKRNDLIFDRDVE
                     RIFVMEPEGQIKLQNLLNQIDERFLFAAETIIDHAETVLMEKLNEHVLIALTDHIAFS
                     AENINNGIVIRNKLLREIEVLYSDEFSIAQWAVEYLTKELGIPYTYDEAGYIAIHIHS
                     GRSGRNNNHRSIREVTIVSDIIHLVESELETDIHSEPFSLNYSRLVNHLRLLLQRTHA
                     QQYAVLDTEIVEMVKRKYPESYKISKKIRVLLTKEYQLAITKEELGYLAIHIERLRSA
                     IVQTNVNNHEEEKN"
     gene            complement(11023..11895)
                     /locus_tag="IXT20_002976"
     CDS             complement(11023..11895)
                     /locus_tag="IXT20_002976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAQ4861335.1"
                     /translation="MDIRILTYFLAVAREKNISRAAKSLHITQPTLSKQLKDLEEELG
                     VTLFTRGSREIQLTDSGRYLYSKGKEILSLVEKTANNLTNDAIISGEIYIGGGETKAI
                     SFISDSLHSLIKAHPDIQFHLYSGNADDIIDKLDSGLLDFGLVIEPTNKQKYQSFRLP
                     AQDRWGLLIHRNHPLAKQTVIHPQELKTVPLIISRQTSVDSQLAEWLGFSIDQLSIVG
                     TYNLLYNASLMVDNGTFGALALDGIINTYGTEKIFIPLDPPLQASLNLIWKKDQPLSP
                     AARKFLDVLTEKYS"
     gene            12023..12592
                     /locus_tag="IXT20_002977"
     CDS             12023..12592
                     /locus_tag="IXT20_002977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HAQ4861336.1"
                     /translation="MTSLIEKQIVNKEIRVTDPLFEVIHQIQAENEEPLAALNTGYHE
                     PADIRKRLENIISDKVDDTVTVLLPFYTDFGKHISIGKNVFINRQVMFVDLGGICLED
                     SVLIGPRVNLITVNHLTDPKERRGLSVKPIHIKKNAWIGAGATILPGVTIGENAIVAA
                     NATVTKDVPDNTIVAGIPAKIVKPVERII"
     gene            12659..13396
                     /locus_tag="IXT20_002978"
     CDS             12659..13396
                     /locus_tag="IXT20_002978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAQ4861337.1"
                     /translation="MNDLSEKVIVIMGASSGIGEATTKLLAEKGAKLVIAARREDRLK
                     AIKESLPEAELYIQTADVRDFAQVQAVIDLAMEKFGRIDVLYNNAGIMPTAPLVEGHR
                     DEWQNMLDINIMGVLNGISAVLPIMEKQKSGHIISTDSVAGHVVYPDSAVYCGTKFAV
                     RAIMEGLRQEQRQNNIKSTIISPGAVQTELYQTISDKKAALELHEAQKEWGLTSEDIA
                     SAVAFAIETPDRMSVSNMIIRPTTQEV"
     gene            13627..14895
                     /locus_tag="IXT20_002979"
     CDS             13627..14895
                     /locus_tag="IXT20_002979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-glycoprotein
                     endo-beta-N-acetylglucosaminidase"
                     /protein_id="HAQ4861338.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGSTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
     gene            15382..15846
                     /locus_tag="IXT20_002980"
     CDS             15382..15846
                     /locus_tag="IXT20_002980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAQ4861339.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIEH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            15830..16459
                     /locus_tag="IXT20_002981"
     CDS             15830..16459
                     /locus_tag="IXT20_002981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HAQ4861340.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMQPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITTFE"
     gene            16813..18291
                     /gene="msr"
                     /locus_tag="IXT20_002982"
     CDS             16813..18291
                     /gene="msr"
                     /locus_tag="IXT20_002982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Msr family ABC-F type ribosomal protection
                     protein"
                     /protein_id="HAQ4861341.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRPDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            18338..18784
                     /locus_tag="IXT20_002983"
     CDS             18338..18784
                     /locus_tag="IXT20_002983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HAQ4861342.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(18983..19498)
                     /locus_tag="IXT20_002984"
     CDS             complement(18983..19498)
                     /locus_tag="IXT20_002984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAQ4861343.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            19615..20816
                     /locus_tag="IXT20_002985"
                     /pseudo
     CDS             19615..20816
                     /locus_tag="IXT20_002985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
BASE COUNT         6631 a         3703 c         4314 g         6214 t
ORIGIN      
        1 caaggagcaa tttcaaaaaa cagcttctca tgtttgatga atattttgac ttgtgaccga
       61 agagttggta ggagaacacc gtttataaga ggttgtgaca aaagtcttgt cgcaacctct
      121 tttttaacca aaaaagtgca aatagtttac tttaagtaag agaataatta tgatgaataa
      181 aaaaggtgga acaaatagca tactatatat attgaaatga acactgtatg gatgctccaa
      241 cttgatgaag ctaaggagga attcgtatgt cagaaaagga attaatgaag gtagtcggtt
      301 tttatgaagg gttgccgatc gatgatccaa aaagctttat tgatgaaaaa gaacagatac
      361 ctgtacccag ttcacgggat cttctggtaa aagtcaacgc agtatctgtt aatcctgtcg
      421 atacgaaact tcgccaagat aacgggataa gaaatgcttt acgtatctta ggttttgatg
      481 gagtcgggaa agttgttgct gtaggtgacg aagtgcaaaa attttctgtc ggcgatcgcg
      541 ttttttacgc aggtacaaca acaagagcag gaagcaatca agaataccaa ctagttgacg
      601 aacgtattgt cgctcttgca ccaaaaaatc tttcagatga agaagctgca gccttgccgt
      661 tgacctcatt gaccgcttat gaattgttat ttgaaaaatt tggtctaact ccagaagaaa
      721 atgccaatcg cgggaaaaaa atcttagtga tcaacggatc cgggggcgtc ggttcgattt
      781 tgaatcaact agcgcattgg gctggcctag aagtctacgc gactgcaagt ccgaagaatt
      841 ttgaatggct gaaaaaaaca ggtgtagact atccaatcga ttatcatcac gacttaagta
      901 aaagttttcg tgaattagga atcgatcaag tagagtatgt cgctgtatta ttcgatatta
      961 cccgctactt tgatcaaatc aagcaccttg tccgaccatt tggacatgtg ggaacgatcg
     1021 tagggatcaa agagccgtta gatataggtg tttggaaaaa ccaatctatc agcttcgact
     1081 gggagtatat gttcgctaaa acagactata actatgaaat cgaaacgcaa ggagaagctt
     1141 tagcgcacat tgctgcttta gcaaatgaag ggattatccg cagtacattg agcaaggtct
     1201 attcagatgg gatgaatgcg aagaacttga aacaagcaac caaagacgta gaaacaggac
     1261 atatgttagg aaaagtcgtt gttagtggtc cgttcaacgg ataagcaaga atacgccgat
     1321 ttttttctta tttgactttg ataaaaacaa gagcgcatga cagtaatttg ccataagctc
     1381 ttgttttttt atttataata agcgaatcaa cgaaagggaa agaattcctg ccaagacgat
     1441 caccaataag ctttttgaaa gaatagcagc taaaagtgta ggaactgaag ccaaagcatt
     1501 ttcgagattg atctgcggca gatgtccgat attttgggta aatagactgc tgaaccataa
     1561 agcagacata atgacgatcg ggacaaaact taaatactca agcaacggtt gcggtaaatc
     1621 gaattttttc agtaagacaa atggtaggac cctagaaagc caagttgcta aagtacaacc
     1681 taaaatagtc agcaatgtaa actcaaaaga aggcatgttt gatccacacc cccaatccgc
     1741 acccaatcag tgttacaact acaatcacta agttgcttgg gataaaaatc agtccgacat
     1801 ataccagaat caaagtgaga ccaatcagga tcaactgtaa tcgtttagag gtgtttctat
     1861 cagaaatgac ttgaagataa agtaacccga taaacatagc gacgatggcg aaatccaaac
     1921 caaacttttt cggatcagta ataaaattcc ctaaaagtgc gccaacaaga gaagaaccca
     1981 cccacgtcaa ataagaaatc caattagctg tattcatcca acgaaagttt aacttttgtc
     2041 cagtatagtt caatttattc attcccagtg cgaaactttc atctgtcagc aatgtgccaa
     2101 tcagcagatt ttgaagcagc cgatttttct tgaaataagg agcaatcgtc atactcatca
     2161 gaatcattcg ggaattgact agaaaggtcg agaaaacgat ggaaagcaat gggctgtgac
     2221 tagcaagcat gctgactgtg ataaattggg cagaaccagc atagaccagc agggacatca
     2281 acgtgacgac tagtggatga aatccggcag ctttcccaac gataccaaat gcaagtccaa
     2341 taccgatata accgaaaacg gtaggtaacg tgtctttgat cgcagttttt atatctaact
     2401 tttcatccaa taacaaaact cctttagcac gacataaatt ttcataattg tcgttaaact
     2461 tgtgaatatt ctaaattttt aatgagcatt tgtcaatggt gggattgaaa cgtaaagaac
     2521 tagcgggtcg cagatttaca cgtattgttt cggtgggagg aggcgcgaaa aatcttgcat
     2581 ggctacaaat ccaagctgat attttcgatg caacggtcta ttgtttaagt atggaggaag
     2641 gacctgcatt aggcgcaaca atgattgctg cactagggtt agggtggttc gacacacctg
     2701 aaagttgttg tcagaatttt atccaatata cagatgcagc ggaacctctc ttacaaaatg
     2761 tagaaaagta tcataaaatc tatcaacaat gccggaaaat ctatccagca acgaaggaaa
     2821 tttgtcatga cttataaaac aaaacgcgta cctaatagtc aaatgactaa tattgggtac
     2881 gcgttaagtc ttctttactt catcctttgc atgattcgtg tttttaaagc aaggtctgct
     2941 gtatttcttg tggatttagg atggttgtta tcgtttactt gctatttttc attgaaatcg
     3001 tctttcttta ccgtttcttt agttttttat ctattttttt cttttggtta gaaaattctt
     3061 tttcccgaaa gtcttctttc aggattttta tagagtagcg ttttgaataa gaggcgtcag
     3121 tatgatcgac ttggcagacg aaccaagaga acatttgcct tttttctgca tgaggtgctt
     3181 ctgctaataa ttctttccat aaataataac agtcctctat cagaagaagg gttcgttcat
     3241 cctcaggctc ccaatagaat gtttcgagag tagcaatcac ttcattgatc aattcgaatg
     3301 cgggaagata ggcattttgc tgaataagcg attcaactgc ttcttggatg aactttcgca
     3361 tctccatgat gaacttcgct gttttcctgt ttttgttttt cttgataata tgatcgtatt
     3421 tatggatgat agttgttaat ttatctgaat aatttattgc atgaaatggc atcttaactt
     3481 tgaagcgttc gataaagtgc ggatgttcta taagaatctc tgttaaaagc tcccgtacaa
     3541 gagaggaact agcggaggaa acaagggacg agagttcgga aagtgagtag ttggattttt
     3601 ttattggacg ttcttcgtaa gcgaataaga cagcagccat gtgtttgcat ttttttcctt
     3661 ctatggcata tagacaatca caagtcatgt tggtcttttc atcccaagtg atcactacct
     3721 tataatcttc cgtgccagaa actgttgcgg acaagtactt gcttgtatag ttcaaatgac
     3781 ggacaaaacc gtgcagatga tagctgtaac cgcgttccaa aacgttttca ggaaaatact
     3841 caagccaatt attcatctag ttctcctttc atgaaacact tatttatatt tagtataaag
     3901 aaacgtttaa gaaaaaagta gctgatttta catatttgat aatcactttt gctcgaattg
     3961 tcaaaaattc attctccttt tcttttctta gagaatgtga gaaaaagtaa aagaatagcg
     4021 tgttttaaag atggaacttt ttttgttttt tatttatctg acaaaaatga agtgattttg
     4081 ttagaaataa ataattgttc caattgataa aagaggtatg atgtatcgca aagaaaaatc
     4141 caagcagcgt tctgatcagc agtttgaaca acaatttgct ttgtacagta ggtagtaagc
     4201 ggattaacta agggaataaa agatattttt accgctttgt ttacttagaa tctgtcttcc
     4261 ccaaaattta tttttctaaa ggtaaaacat agataggcag ataataccaa ttatccgtta
     4321 cgaaacgctg cttgagaatt ttctttagat ccatacaaca ttttctacat ctgactaaat
     4381 tggttcctga ttcatcaagt aattgacttt ctttgataat atgataaaaa ggcaatcaag
     4441 gaggaagata aatatgctta aagatctgaa ccaattgatc gattacatcg aagaacattt
     4501 gacagaagga ctttcctata aaataatagc cgaaaataca ggaatctctg aatatcaatt
     4561 aaaacgcatc tttacgttta ttgcagggat gtcgctaatg gactatatca aaaaccgtag
     4621 attggctttg gctaatcgcg atcttctaga tggatcaaga gtgacagaaa cggcgtttag
     4681 atatggatat gaaacagtcg aaggtttttc aagagctttt cgtgattggt caggatatct
     4741 gccttcagaa atcagtagat acggcataca aaaatctttt ccacgcttga cattttttat
     4801 tgatgtcaga ggaggaaaat cgatggaatt tcgtatcgaa aagaaagaac agttttatgt
     4861 ggcaggtgtg gcgaaaaaag ttcctgtcaa atttgaagga gtcaatcaag aaatacaaaa
     4921 gttagcgcag acgatcacgg agactcaaag ggaagagatg cataagttag gagatttata
     4981 tccaaatcaa gtaatcaacg tttcttatgg atttgatgga gaccgcttgg aagaaaaggg
     5041 cagtttgatg cacatgattg gctttgctac gacaaaagaa aatccgtacg ctgatttaga
     5101 aatgcttgct atagaagaac acacttgggc catttttcct aataaagggc catttcccca
     5161 aactttacaa gatacttggg ggaaaatcta tgcagaatgg cttccttctt cagattatgg
     5221 gttagtcgaa gctcccgaga tctcttttac aaaatgggga gaggatttca gtaatgttta
     5281 cagtgagatt tggattgcag tgaaagcaaa aaaatagtta aaaaataaaa atatccctag
     5341 aaaaaagagc gtaatcgtgt catttccctt taattttatg taagatgaac ttaatataat
     5401 agaaagggtg ggagaaatgg caaagtatag gatcgaagaa aaaccagatt ttacggtgac
     5461 gggtattggt gtggaattga agagtagcaa tacggatttt gtcggtttgg ctgcagagaa
     5521 acaagcattt gagcaaaaag ttatcggtaa tggaacaata gatgcattga aacaagctgc
     5581 tgaaaatcat tatttcttta cagtcaacga accctataac gggaaaatga tgtactatgt
     5641 cggtgttcaa acgagtcagg atgttccaaa cactacgcga atcatcccat tcccaaaagg
     5701 aacgtatctt gttgtaagcg ctttagcaga atcgcctgaa gaattaagaa atgatttgac
     5761 gaatacggcc tttgggcatg ttttaagaga agtagaagat tacgcctatg ttgggggtcc
     5821 aaatgctgta gtcgaaatgg gagttaccaa cgggaaatat atgggagaaa tctggatacc
     5881 ggtagtcaat aaaccatgat aatatagagg tcgtgaaagt agcgctttga cctgagcatt
     5941 agagaggaaa aatgggaaat agctttccat attttgcatt tttcagaact aatgccgagg
     6001 gtcagctact tgaacaccgt ttatcaagac gtcatgactt cagcgctttg acctgagcat
     6061 tagagaggaa aaaggggcat gacaaggctt ttgtcccccg gagaataaaa gaatttatgt
     6121 agacgattcc gatcagaaaa atgcaggcac agtagagaga attataaaaa tctctctacg
     6181 aggcctgtgt tttctggtct tttttctttt tccaaaacgg ctattttatg tatcagacta
     6241 tcaagtgtga tcttagccat ttcttcttct gcctttttct gcagccgagc gtagctttct
     6301 tctaaagctt cttggatatt cgcaccaacg acgcaatttg gatttgtttt tggatcgact
     6361 tggataagct gtgtattacc ttcgatgctg cgatagacat ctagtaaaga aatctgatct
     6421 ggacttttgg caagggaagg ctgcggtttt ccggtttttg taaagatcaa accggatttt
     6481 ttgagctgac tcatgatttt acgtacattt gctgcatttg tttcgatact actggcaatc
     6541 atctcactag aaagtgatcg ggtatcttga aacatttcaa tataggcaag tacatggatt
     6601 gcatcactaa attgcagaga atacttcatc agttgtcatc ctttcatttt ttttgcttga
     6661 cttatcttac cacactcttt atgataaagg tgtaatttat aaatgtacat ctgtaaatct
     6721 taacaaataa aaagaatagg gagtggcaaa tatgcattat ttagtaacag gagcaactgg
     6781 aggttttggt ggttatgcgc tggaattctt aaaaaagatg gttccacaaa atgaaatcta
     6841 tgtactagta cgtagtgaag aaaaaggaga agcactgaaa gaagctggat ttaatatacg
     6901 tattggtgat tatgatgatt tagactcaat gaaagaagcg ttgaaaggaa tagatcgatt
     6961 gctttttgtc tctggcgttc caggaaatcg tcaagccgaa cataagaatg tagtgaaggc
     7021 agcacaagca gctggggtat cttatatcgc ttatacaagt tttgcagatg ccgatcactc
     7081 gatcagtgta ttagcgccag atcaccaatt cactgaaaaa atgattaagg attcaggtat
     7141 ttcccataca tttttacgga ataattggta tgtagagaat gaattaccta tcattgggca
     7201 ggctttaaaa actggtcaat tcgtttatgc tgcaggaaat ggaaaaacag gttgggcatt
     7261 gaaacgtgaa tatgcagaag ctgcagcaaa agtactagta gaaagtgact ctccagaaat
     7321 cttagaatta tccggcaatc taactgacta tgaagaatta gccaaggcat tagagcgtgc
     7381 gacaggtaaa gagttagaaa ttattgaagc aagtgatgca gcgtttgtcg aaaatctaaa
     7441 agaagccgga ttccctcaag aagcagctga catgttttta gcgatccaac atgatattaa
     7501 aaaggatcag ttagctattt cctctgatga tttagaaaaa gtattgggaa aaccattaac
     7561 ttctcttgaa gcagcattaa aagaaatatt ggatcaggaa taatgtataa agaggttgtg
     7621 aaaaagccga ttagttttga gtatgaagaa aaattttgtg aaaaagctgt tctgcatcaa
     7681 gtaataagaa gagtcaaata aaaaatagct cttcatatgt ttcgtttagc tggacttatt
     7741 accgcggagt tggcaggaga acgccgtttt tattcggaaa aaggagctga gacaagattt
     7801 ttgtctcagc tcctgtttgt ttatcgataa aaaagtctgg ggaagacgat ggctcaggct
     7861 ttgttggaaa cagagccgat tttatcattt gcttttacat cacgtgcggg ttcgatttct
     7921 aagctttgga ctttgtcgcc atttgtgaat acaacgacca tatccgtgtt tttgcctgca
     7981 gattgaatag cggcaagatc aactaaagca atcaattgac ctcttgcgac tttttgacct
     8041 tcttctacat ataaagtgaa tggttcgcct tttaagtcaa cagtgttgat gcccatatga
     8101 agcaagactt cgataccagc atctgtttgg attccgacag catgtttcgt tgggaaaatg
     8161 tttgtgatcg taccagcgat tggagagaag atttctccat tttctggtaa tacagcatag
     8221 ccgtcaccca tcattttttc cgcgaataca tcatctgaaa cttcagtgat tggaatcagt
     8281 ttcccatttg cgatagcgta aatatcttgt tcgatcgttt caccggcaaa tgtttctgtt
     8341 ttaaccacgg tgtttgtttg tgcaggtcgt gctgcagcac caccatgtag ttttgccatc
     8401 tcatctgcaa cgaattgtac ttctgtacct acgatgactt gcgcattttt tccgtcgatg
     8461 atcttcatac caggtacgcc agtggctttg attttatttt gatcgatcag gcctgtatct
     8521 ttgacttgca aacgaaggcg tgtagtacag ttatcgatcg aagttacatt gtcggctccg
     8581 cctaatgcag cgtagatttt gcttgcttgt gccgcatatt tattgtctga attagtcgtt
     8641 gcagtatcgt catctgaaag atctgcttcc tcgcggccag gagtcatcag atggaatttt
     8701 ttgatagcaa agttaaagcc aaagtagtaa atcactgctg ttactaatcc ttggacgatc
     8761 aacatcaatg gctgattcgc aatcggattt ttcaaagata ggaagtaatc gacaaatccg
     8821 gcactaaagg caaatcctgc tgtccaatgg aagaatgcac tcactgctaa agaaatccca
     8881 gtgaaaattg catgcaagac atataatggc caagcaacga acataaatga aaattcaagt
     8941 ggttctgtca caccagtaaa gaatgaagca aaagctgctg ctagcatcaa ggaagctgtt
     9001 tttgctttgt tttctggacg tgcatttctg taaatagcat atgctcctgc tggaagcccg
     9061 aacatcatga ttgggaaaaa tccagcttga tacattcctg ttacgccttt tgttccttca
     9121 gaagccaaaa atttaccaat atcattgatt cctgctacgt caaaccagaa cacggagttc
     9181 attgcttgat gcaatcctgt agggatcagt aaacggttga agaagccata aagacctgca
     9241 ccaccccagc ctaaactcaa aatgaattta gcaaaagaaa ctaaagcaga gtaaaccgga
     9301 ggccatacga gcagtagcac tgcactgatc agcaacatga caaaggcagc caagattggc
     9361 actagacgct tgccgctaaa gaaagataaa gccatcggta attttgcttc gtggaaacga
     9421 ttataaagag ctgccgcaat caatccggca ataataccaa taaatacgtt actgatctga
     9481 gcaaaagatg gatcaactga ttctggtttg atattcaata aagtagctac tgaatcagtt
     9541 tttaaaacat ttactggaac ttcaaatgcg accaaacctg ccaaggcagc agcacctgat
     9601 ttgtccactg acatccctag tgctaaccca acggcaaaca gcaaaggtaa ttgacctaaa
     9661 actgcgttac cgccagcttg taaaaaatga gcgacaatgt cgttactata agatgcccac
     9721 cagtttccga tccctactag taaagcagca gcaggtaaga ccgcaactgg aagttgaagg
     9781 gaacgtccca ttcgttgtag atatgctttc aatttttttc ctcctcatga ttgttaacat
     9841 ttgtttgtac gattgcgctt cttaaacgct cgatatggat ggccaagtaa cccagttctt
     9901 ctttcgtgat cgcgagctgg tattctttgg tcagcaacac acggattttt ttagaaatct
     9961 tatagctttc tgggtatttt cgtttgacca tttctacgat ttccgtgtct aaaacagcat
    10021 attgttgcgc atgggttcgt tgtagaagca gtcgtaaatg attcaccagt cttgaataat
    10081 tcagtgaaaa tggttctgaa tggatatctg tttccaattc tgattccact aaatggatga
    10141 tgtcagatac aatcgtcact tcacggatac tgcgatggtt attgttccga ccgcttcgtc
    10201 cgctgtgaat atggattgcg atatatcctg cttcgtcata agtatatgga atcccaagct
    10261 cttttgtcag atactctact gcccattgag caatactgaa ttcatcactg taaagcactt
    10321 cgatttctct taaaagtttg ttgcggatga cgataccgtt attaatgttc tccgcagaaa
    10381 aagcaatgtg gtcggtcaaa gcgatcaata catgttcatt caacttttcc atcaaaacag
    10441 tttccgcatg atcaatgatc gtttctgctg caaataaaaa gcgttcatca atctgattaa
    10501 gtaaattttg caacttgatt tgaccttctg gttccatgac aaatatccgt tcgacatcac
    10561 gatcaaaaat caaatcattt ctttttctgt caaatccaat cccttttcca atagctactt
    10621 tttcctgtcc accgtcatct accaatactg cattttgatt aaagatcttt ttgattttca
    10681 tgcgcgcacc ctttttgaag atttggtttt cacgtttgct agaccagttg attgcggcca
    10741 aacaaaaata gacacgaaaa acctcctagg gaaaccatgt tcctcagaga tttttcatgc
    10801 ctagtatgat ctagtcacat gaaacgaatt attgttaacg taatcaattt tatatagaca
    10861 gttatgtttt gtcaattctt tttttataat tatagtaatt tactggaatt atcagaaata
    10921 aaaggaagaa ataaattaag aaacttatgt tatttaatcg ttggatcttt tgcttatctt
    10981 attcgttgtt aaaaagctga aagcgaagga aaaaaactct acttaactgt atttctccgt
    11041 taggacatct aaaaatttac gagcagcagg agaaagaggc tggtcttttt tccaaatgag
    11101 attcagtgaa gcttgtaatg gcggatcgag tgggatgaat attttttccg tcccataagt
    11161 attgatgatc ccatctagag ctaatgcacc aaaagtcccg ttatcgacca tcaaagaagc
    11221 attgtacaat aagttatatg ttccgacgat cgataactga tctatgctga aacctagcca
    11281 ttcagctagt tgactgtcca ctgacgtttg ccgggaaatg atcaaaggaa cagttttcag
    11341 ttcctgcgga tggatgacgg tttgctttgc taaaggatga tttcgatgta tcaaaagtcc
    11401 ccaacgatct tgggctggca gtcgaaatga ctggtatttt tgtttgtttg tcggttcgat
    11461 tacgagtcca aaatcaagta atccgctatc cagtttgtct atgatatcat ctgcattccc
    11521 gctatataaa tgaaactgga tatcaggatg cgccttgatc aacgaatgaa ggctatcaga
    11581 aatgaaagag attgctttag tttcgcctcc gccaatgtag atttctccag agatgattgc
    11641 gtcatttgtt aaattgttcg ccgttttttc taccaacgac aagatttctt ttccttttga
    11701 ataaagatat cgaccagaat ccgtcaactg tatctctcgg gagccccgag tgaaaagtgt
    11761 cacaccaagt tcttcttcta aatctttcag ttgttttgag agagtaggtt gcgtgatatg
    11821 cagagatttt gctgctcggc tgatattttt ttctctagct actgctaaaa aatacgtcag
    11881 tatacgaata tccatttttt ccctcctgtt tttaaaaaag cataccacta agtatagtta
    11941 aaaggaatag ttaagtatgt tttataagta ttagacatga taagccgtcc gttataaagt
    12001 aaggataaaa ggagggagca aaatgacaag cctcatcgaa aaacaaatcg tcaataaaga
    12061 gattcgagta acggatccgc tctttgaagt gatccatcag atccaagctg aaaacgaaga
    12121 gcctttagca gcgctgaata ctgggtacca tgaaccagca gatatccgca aacggctaga
    12181 aaatatcatt agtgacaaag tagatgatac ggtgactgtt ctgctgcctt tttatacaga
    12241 tttcggcaaa catattagta tagggaaaaa tgtctttatc aatcgtcaag tgatgttcgt
    12301 tgacttaggt ggcatctgtt tagaagattc agtattgatt ggtcctcgtg tcaacctcat
    12361 cacggtgaac catctaaccg atccaaaaga acgaagaggc ttgtcagtaa agccaatcca
    12421 catcaagaaa aatgcttgga tcggagcagg agcaactatt ctgccaggcg taacgattgg
    12481 cgagaatgcc attgttgcag cgaatgcaac tgttacgaaa gatgtcccag acaataccat
    12541 cgttgcagga atccctgcca aaatcgtcaa acctgtcgaa cggattattt gaacagtaag
    12601 atgaaataag agatagtgag tataagcaag agatttttca aaacaggagg agaatagaat
    12661 gaatgattta tcagaaaaag tgattgtcat catgggcgct tcaagcggaa tcggtgaagc
    12721 aacaacgaag ctattagcag aaaaaggggc taaactagtt atagctgcac gccgagaaga
    12781 tcgcctaaaa gcaataaaag aatctttacc agaagctgag ctatatattc aaacagcaga
    12841 tgtaagagat tttgcacaag tccaagcagt tatcgattta gcgatggaaa agttcggtcg
    12901 aatcgatgta ttgtacaata atgcaggaat catgccaacc gctccattag ttgaaggtca
    12961 ccgtgatgaa tggcaaaata tgcttgatat caacatcatg ggtgtgttaa atggaatttc
    13021 cgccgtactt ccaatcatgg aaaaacaaaa atctgggcat atcatttcaa ctgattctgt
    13081 ggcgggacat gttgtttatc ctgattccgc cgtttattgc ggaacaaaat ttgctgtccg
    13141 tgcaatcatg gaaggattac gtcaagagca acggcaaaat aacatcaaat ctacgatcat
    13201 ctctccagga gctgttcaaa ctgaattata tcaaaccatc agcgacaaaa aggctgctct
    13261 tgaacttcat gaagcgcaaa aagaatgggg attgacctcc gaagatatag cttctgctgt
    13321 agcatttgcg atagaaacgc cagaccggat gtctgtcagt aacatgatta tccgtccgac
    13381 gacacaggaa gtataatcca taacggataa tatccaaaga gaattacttg ttaaatagtt
    13441 attcaaacga ccatcaattt aaggcgtgaa tggagaagaa tattcttcat ttacgtcttt
    13501 ttatgtagaa aaagcaaatt tatcaaaaag aaacaaatgt tatttaaata tggacattga
    13561 cttgctttta cttttttagt tacactgtta tttaggaaaa gtttcataat tcaaaggaga
    13621 aaaaagatga aagcaaagaa tttattattt agcgttattc ttttaggaaa tctagcagtt
    13681 gccaacacaa cagtgctagc tgaagaatcg acagaaactg tcgcaactca gacatcgagt
    13741 tcttcagaaa caggaattga aaacgataca gagagtactt tgcagtcagc tactgtctca
    13801 gaaaatacaa tttcaaccga agaaccaaca actgaaacaa cagaacaaac acaaaccgaa
    13861 gacagtacat cagcagaagc tcagaacaat ccagagaatg cagaagaaat cacgaaccaa
    13921 ttgacagatg ggcaaacatt gacacatacc catcaagcag atttgtctga attcgaaaac
    13981 tatactggct atgcttttta tgctcgctca tctgctaatt ctcaacaagc gttcattgat
    14041 agtattgcct caactgcaca aagtttggca agtgccaatg acttatatgc atcagtcatg
    14101 attgctcaag ccatcgttga aagtggctgg gggaacagta ctttggcttc tgcccctaat
    14161 tacaatctat ttgggattaa aggaagctat aatggccaat ctgttacgat gcctacttcg
    14221 gaatatgtga atgggcaatg gattacggtg aatgccgctt ttagaaaata tccttcgtac
    14281 aaagaatcat tgcaagataa cgtaacggta ttgaagacga cttcttttca gccaggcgtt
    14341 tactactatt ccggtgcatg gaaaagcaat acgaactctt acaaagatgc tacggcttgg
    14401 ctaacaggtc gttatgccac tgcgcctaat tatggttcta cattgaacaa tgtaatcgaa
    14461 acctataatc tgacacagta tgatacagca ccaacttcaa cttctccgat gtatcgtctg
    14521 tataaccgtc atacaggaga acatttatat acattgaatg ccggagaaaa aaattatctg
    14581 ccaactgtag gctgggaata tgaagggatt gcttggcaag caccaaacag cggacaacct
    14641 gtctaccgtt tatacaatcc ttacagtgga gaccatcatt acacaatggc acaatctgag
    14701 atcaactctt taacaaaaat cggttggcgt tatgaaggtc taagttttta ttctggtggt
    14761 tccaagccaa tttatcgact atttaatcca aatgaaaaaa ctggtaccca tcactatacc
    14821 ttgagtgcta aggaaagaga ttggctaaca ccgatgggct ggagatatga aggaatagga
    14881 ttttatggtt attaaaaaga ggtagtgaaa aagctgccct gcatcaagta gtaagaacag
    14941 ccaaacaaaa acagattttc atgttttttg tttggttgaa cttattaccg cagatgcaag
    15001 tttttgaaca ccgtttactc aaaaaaggtt gtgacaacat cttgtcataa cttttttatt
    15061 actgtttaag catactgatc caatttctaa tattcgacgg gattcttgca aaaggcgatt
    15121 tcggaaattt ttggcggtgt aaaattttct actgcagaag agctatcttc tatagtttga
    15181 taagaatcaa taatcttggt tttagcgttg tgctttcttt atcatttttt ccgttcattc
    15241 tacaaaaaat ttcttttctc tatacttttc tattgagagc gtttttttta ttacacagct
    15301 gattctttga cttttttctt tttttattct attattgaca agtcaatcgt tgacgagtca
    15361 attattagaa aggaggaaat tatgtcttta gaagagatta gtcacctttt atatcaaatc
    15421 aaattagccg atcaaaaaat cgtccagatc tttgaagaaa atatcggtat aagtcttact
    15481 agatacgaga tcatgatgag actgaaagaa gaaaatccat tgctacaaag cagtttgcaa
    15541 gaaaagttgc agattgatca aggagcagtt actcgtcatc tgaaggtatt ggaagcaaat
    15601 ggctatgttt cgcgtcagag aaatcctcag aacaaccggg aagtgtatgt agcgctaaca
    15661 gaaaaagccc agaagaaaat cgagcattgt gaaatggacc ataaagatac aggttccgtg
    15721 ctatccccgg ctttcagcga tgtagaaatg aaacaatttc taacactttt agataaattg
    15781 aatacccaac tttgcaatat aacaacagaa acgaaagagg ttaataataa tggaacaaaa
    15841 aaatgatttt tcaacaatta tgactggtag aagatccgtt cgcgtatatg atgaaacata
    15901 caaaatccca cacgaagaaa tgctggaaat gatcaaagaa gcgacgactg caccttcttc
    15961 tgtcaatatg caaccatggc gctttgtggt cgctgaaagc gaggaagcaa aagaaatctt
    16021 gcgtcctttg atccgtttca atacgagaca aaatgatact tcttctgcaa tggtcatgat
    16081 ttttggcgat atgcaatgtt acgaatatgg cgaagaaatc tataaccaag cttacgaatc
    16141 agggaagatg ccaaaagaag tcagagatca acaattagct gcgatcattc cttattacaa
    16201 gagtttttca agagaagaaa tgaacgatgt agtcaaagtc gattctagct tagcggctat
    16261 gcaattcatg ttagttgcgc gtgatcatgg ctatgaaaca aatccaattg gtggattcga
    16321 agcagatcag ttggcagagg catttggttt ggataaagac cgttatgttc cagttattat
    16381 tttatcaata ggaaaagcgg tagaagaagg ttacgagtct gttcgtttag caccagaaaa
    16441 gatcactacg ttcgaataaa aggttttcag ttactattca gtagctattg ctttttttga
    16501 atcgtgtgtg gtaacctaat aaaaaaggag gtcggaaaaa tgactgcatc gatgaaatta
    16561 cgtttcgaac ttttgaataa caactaaata aattcaaaag acatgtataa acaacaattt
    16621 catggatgga gtatgtccat tttttcgaac gacagtagaa taggtgaaag ctatgacaga
    16681 tttggtgatt tagtcatagt tatttcgttc agagtaggaa aagatgtgat gcagtttttt
    16741 tgcaaacaga tttttctgct cttttttagt atcgataatt gttgttttac atgaaatagg
    16801 agtgtaaaac atatggaaaa tttagcagta aatataacaa atctacaagt cagctttggc
    16861 aaccagctag aactatctat tgattctctt cgtgtctatc agcaagatcg tatagggatc
    16921 ataggagaaa atggtgtcgg taaatcgact ttgctcaaac taatagccgg tgaacttttt
    16981 cccgatcatg ggaaaatcca aacagagatc accttcaact acctgcctca attaacctat
    17041 ctcgctgaag caaaggacct aaatttggaa ttagccagtc atttccagtt aagactggaa
    17101 gaaacttcag agcggaaatg gagcggaggg gaagaacgaa agatcgagtt gatacgtctt
    17161 ctttcttctt atgaacaagg gatgcttcta gacgagccaa caacccatct agatagaaaa
    17221 agtattgatc gactgattga agagcttcgt tattattatg gtacgctggt ttttgttagt
    17281 catgatcgct attttctaga tgagctagca tcgaaaatct gggaagtaaa agacggagaa
    17341 atccgagagt tttcggggaa ttatagtgcc tatctcactc aaaaggaatt ggagaaaaag
    17401 actcagctac gagaagcaga gtcgatcatg aaagagaaaa aacgattgga aaaatcgatc
    17461 caagaaaaga aaaaacaagc ggaaaagttg gaaaaagtgt ccagtaaaaa gaaaaagcaa
    17521 caaatcagac cggatcggtt gtcttcctct aaacaaaaag acagtgtaca aaaagccatc
    17581 caaaagaatg cgaaaacatt agagagaaga ctccaaaaaa taggagaaac aaccaagccg
    17641 cagcagatga aacaaatccg ttttccagta ccaaaatctc ttgaactcca cagccgctat
    17701 cccatcatgg gacaaaatgt ccaattggaa agaagtggga gaacattact ggtaaatggt
    17761 gattttcagt tttctttagg taaaaaaatc gcgattgtcg gtgaaaatgg ttcaggtaag
    17821 acaaccttat tagaacatat ccgcaaacaa ggagaaggaa tccttctctc tccgaaagta
    17881 agctttcaag tatatcagca aaagggttat caaatgacat ctgaagaatc catcattcgt
    17941 tttgtcatga gacaaacaga gttttcagaa tcgcttgtcc gtagtttgct gaatcactta
    18001 gggtttgctc aggaaacttt gacgaaaccg ttatgtacat taagtggggg agaagcgacc
    18061 cgtctgacga ttgctttgct ttttactaag ccaagtaatg tgttgctgtt agatgaaccg
    18121 actaatttta ttgatatggc aacgatcgaa gctttagaga agctaatgca aatatatccg
    18181 ggaacgattt tgtttacttc tcatgattct tactttgtcg agcgtacggc tgatgaagtt
    18241 tatgaaataa aagggcagaa aataaaaaaa gtacttacga gaaattttta aatgtgctac
    18301 gatgattttc gtgacaaatt taaaggagtg gtacagattg cctacaaata aaaaagaagg
    18361 aataattttt acaacaatga tgtgtttttt gatggtcttg gggatgagcg cgtataattt
    18421 atggctgcat caagatcttc atttatcaag tttgttgatt ggtctagtgc ctggattcgt
    18481 ggtggctttc attttggatg tcttcatcat cggtgtgctg gcgaaaaaaa ttgcctttaa
    18541 actaccagta aatccagaaa gcaaggtcca aatgatctta actatttcat gcttgatggt
    18601 tttaggaatg gtgacatgta tgtctttatt cggtgtgatc atggaacaag gaatacctga
    18661 tcaattattc tctgcttata tgaatgcttg gaaaatgaat gtgattgccg ctttaccgtt
    18721 gcagttgatc atcgttggcc cgctttcaag aagtgtgcta aaagcaattc aaagtaatga
    18781 atgatggaac actgttattt gaaaaaaggc cttggcatgc tttttgtcat agtctcttga
    18841 atgcagttct agaaaaatag tgatccgaac gataggtttt ggcctgtcgt tcggatcgct
    18901 ttttcttttc caactgtatt gctcttttat tgaagataat ttgctcttca atagatattt
    18961 ctagaaaggt tctgtttatc ccttactcaa aaaactgctg catttgttct cttacttgct
    19021 gacaccattg gagttcctgc tgacaaagca taattctgcg ctggatcaca acataacgtc
    19081 caaattcttg tttcttgtct agcgagaggt cattcaattt tttttgattt ttttgcatga
    19141 ttttgttcag ctgttgttct ctagtgaata gcagtccagt agctgttgtt ttatcgagcg
    19201 tcgaaaaagc atagattttt gctagaaatt catctttttg cgtaggagcg ggagtttcct
    19261 ctttgatcca ttcctctaca agagcacgtc cttcatcagt caagtggtag atcttttttt
    19321 gagagtgctc gtctttgtcc aaaacggtca cataaccctg ctggtctaac ttggcaagcg
    19381 ttgtatagat ctgactgtga tgggcttccc aaaaaattgt gatcagctgc ttcaattcat
    19441 acccagaacg tggtttggat aaaagcacgc ataataatat ataggctaaa gtattcataa
    19501 aaatcctacc tctcgtctta ttttctttat tatatcacag taaagtttcc acgttttaca
    19561 tgtgcatatt tacatgtaaa ataaatcatg tatgatgcta atagagagaa agggatggaa
    19621 atattggaga atcaaataga tatacaagaa caaacaaatg aagaaacaaa ggggaaaagt
    19681 tcagcgctag ttgtagcagc tacctgtatg atcgctttta tgggagtggg tctagtagac
    19741 ccaatcttaa aaacgattgc tgcacagtta aacgctacgc cagctgaaac gaccctgtta
    19801 tttactagtt acatgttagt cactggtgtg gtgatgctat ttacaggttt cttatctagt
    19861 cggatcggtt tgaaaaaaca ttgatgacag gattgtttat catcgttgta ttcgctggtc
    19921 ttggcggatt atctgggaat atcggaatgc tgattgggtt acgtgccggc tggggtgtcg
    19981 ggaatgcttt atttgtctct actgctttag caacgatcgt cagtatttta gttggtaaca
    20041 cagaaaaagc aatcatgatg tatgaagcag cacttggctg cggaatggct gttggccctc
    20101 tagtcggtgg ggtcttgggc agtggttcat ggcgttatcc attttttggg gtagcatttt
    20161 tgatgttttg cggttttata gccctagcgg tacttttacc tgaaactgag aaaccaaaga
    20221 gaaaagtagg cctttgggaa ggtgtcaaag cgttaagcaa tcataaactt cgttcagttg
    20281 gactaatcgc attgttatat aatttcggat tctttacttt acttgcgtat gcgccatttt
    20341 tattgattgg ctattctgaa ttagaagtcg gttttgtatt ttttggctgg ggtgtgcttc
    20401 tggctattgc ttcgattttc gttgcaccgg ctcttgagcg gaagtttacg acttatcgga
    20461 caattttagc agcatttgtc ttattcatta tttgtttgct gttattagga gctggaactt
    20521 ctatgccagt ccttgtaccg atcagcattg tgttagcagg atttttccaa ggaattatca
    20581 atacactgtt gacgacgatt gctatggaaa taccaggtct gcaacgaaac gttgcttcgt
    20641 ccagctatag ttttgtccgc tttttcggtg gagctttagc accatttatt gcaggtgtga
    20701 ttggtgaaca tattggtgaa agctatgcgt tttattttgc aggtgccatt gttcttatca
    20761 gtgtatgttg tatcatttat caccgtaatt attttgctac aaatgaagaa gaataagaaa
    20821 tcagaggttg tgaaaaagct gtcctgcatc aagtcataag aa
//
LOCUS       DACGLE010000214        20164 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0534
            SAMEA3304449-rid9560453.guided.047, whole genome shotgun sequence.
ACCESSION   DACGLE010000214 DACGLE010000000
VERSION     DACGLE010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304449
            Sequence Read Archive: ERR985064
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20164)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20164)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702225.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702225.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 20:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,045
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 27 of 67
            Pseudo Genes (incomplete)         :: 25 of 67
            Pseudo Genes (internal stop)      :: 19 of 67
            Pseudo Genes (multiple problems)  :: 4 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20164
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304449-rid9560453.guided.047"
                     /strain="VREN0534"
                     /db_xref="taxon:1352"
     gene            9..1049
                     /locus_tag="IXT20_002986"
     CDS             9..1049
                     /locus_tag="IXT20_002986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000288739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HAQ4861344.1"
                     /translation="MYTSINGELCKIDRAYSGEIVILQNEFLKLNSVLGDTKLLPQRK
                     KIENPHPLLQTTVEPSKPEQREMLLDALLEISDSDPLLRYYVDSTTHEIILSFLGKVQ
                     MEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVSPLP
                     LGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYSPVS
                     TPADFRMLTPIVLEQAFRKAGTELLEPYLSFKVYAPQEYLSRAYNDAPKYCANIVNTQ
                     LKNNEVIIIGEIPARCIQDYRNDLTFFTNGLSVCLAELKGYQVTTGEPVCQTRRLNSR
                     IDKVRYMFNKIT"
     gene            1147..1335
                     /locus_tag="IXT20_002987"
     CDS             1147..1335
                     /locus_tag="IXT20_002987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011988843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAQ4861345.1"
                     /translation="MINYKWILCPICGNKTRLRIRVDTILKNFPLYCPKCKNETLINV
                     QKMNIITIKEPDAKTQSR"
     gene            complement(1393..1746)
                     /locus_tag="IXT20_002988"
     CDS             complement(1393..1746)
                     /locus_tag="IXT20_002988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ4861346.1"
                     /translation="MRKKEDKYDFRAVGLAIKEARMKRGLTREQVGTMIEIDPRYLTN
                     IENKGQHPSTQVLYDLVSLLHVSIDEFFLPTDNLIKSTRRLQVEKYMDSFTDKELSLM
                     EALAKGINEARNIEG"
     gene            2277..2699
                     /locus_tag="IXT20_002989"
     CDS             2277..2699
                     /locus_tag="IXT20_002989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000804881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAQ4861347.1"
                     /translation="MKPSSFQETIENQFDYICKQVIEDERKDYFKHLDRLSAKEVSFS
                     DIDNYLVNSFSTVDTYVTDFQLFKLFGLRINVESDLLSEALRHLSEKKRNIILLHYFM
                     DMSDVEIAELLNLNRSTVYRHRISGLAMIKEFMKECKE"
     gene            2696..2926
                     /locus_tag="IXT20_002990"
     CDS             2696..2926
                     /locus_tag="IXT20_002990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ4861348.1"
                     /translation="MKTAYPRVPFPLIVKATDGDVEAINQIVKHYRGYTSKRSLRRMT
                     DEYGNSHMVIDETLRGRMETKLISKILSFEIK"
     gene            3424..3624
                     /locus_tag="IXT20_002991"
     CDS             3424..3624
                     /locus_tag="IXT20_002991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008475635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HAQ4861349.1"
                     /translation="MITVTKKDLVELGYGTSFSADIIKKAKELMITKGHIYYQSRKLD
                     RVPREAVEEILGIRFSNENRSE"
     gene            3651..4844
                     /locus_tag="IXT20_002992"
     CDS             3651..4844
                     /locus_tag="IXT20_002992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAQ4861350.1"
                     /translation="MVKDPIKKAKNGTYYFRANLGYDSKGKKIQKYRSGFTTKKEARE
                     EYSKLLLMKPEELSENKDKMTFKHYIFEIFLPWYKTQVKLRTYENRLPTIKKHFSYFD
                     KMAVSDIEPIDVQNWQLKLSKKCKSSYVRAVQGLFSVAMDRAIVLGITTTNPSKIIGN
                     VKKQKSKIEFWTKEEFEKVISLIYKEDYYQHFLFISLWFLFMTGMRIGEATAIQWEDI
                     DFDSGVLTINKTLYYKNQSNYRFVEPKTKASVRHIVLDKCTLTYLSEWKEVQQNLIQT
                     DFVMSYNGIPTQKHTLSYAIERYAKLAGIHRIRIHGLRHSHASLLISMGENPLIIKDR
                     LGHEDIETTLGTYGHLYPNSNFEVANKLNGAISFKEAKQNYDTSPKNQFTVDYLRNKE
                     MKKVQ"
     gene            complement(4912..6477)
                     /gene="guaA"
                     /locus_tag="IXT20_002993"
     CDS             complement(4912..6477)
                     /gene="guaA"
                     /locus_tag="IXT20_002993"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HAQ4861351.1"
                     /translation="MTNVADMTNVEKIIVLDYGSQYNQLITRRIRDLGVFSELLSHRI
                     TAEEVKAMNPKGIILSGGPNSVYDENAFGIDPEIFNLGIPVLGICYGMQLITYKLGGS
                     VEPAKNREYGKALLEVTSDQAVLFKETPKQQTVWMSHGDLVTKVPEGFETVGTSKDCP
                     IASIQDTTRNFYGIQFHAEVRHSEYGIELLRHFAFDVCHCEGNWTMDNFIDMQVEKIR
                     EQVGDKKVLLGLSGGVDSSVVGVLLQKAIGDQLTSIFVDHGLLRKGEAEQVMESLGGK
                     FGLNIIKVDARDRFLDKLKGVSDPEQKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQG
                     TLYTDVIESGTETAQTIKSHHNVGGLPEDMQFELIEPLNTLFKDEVRELGTQLGMPDS
                     IVWRQPFPGPGLGIRVLGEITEDKLEIVRESDAILREEIAAAGLDRDIWQYFTVLPGI
                     RSVGVMGDGRTYDYTVGIRAVTSIDGMTADFARIPWEVLQKISVRIVNEVAHVNRIVY
                     DITSKPPATVEWE"
     gene            complement(6536..7459)
                     /locus_tag="IXT20_002994"
     CDS             complement(6536..7459)
                     /locus_tag="IXT20_002994"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HAQ4861352.1"
                     /translation="MNDPMNYYRVAREEWREFYRNGQAPLTQDELDNIKSLNDRISMQ
                     DVRDIYVPLAHLIYLYMKEFESLFLSKGLFLHKYVEVPPFIIGIAGSVAVGKSTTARL
                     LQTILSRTFKRRNVQLITTDGFLYPNRILKERGIMDRKGFPESYDMEMLIDFLNRVKS
                     GQEEIKVPVYSHDVYDIIPDKYELIQQPDILIVEGINTLQLPANQQIYVSDFFDFSVY
                     VDADPVLIEKWYLDRFGALLDTAFQNPENYYYRFAIGDREEAFKMAKDVWKNVNLKNL
                     QEYILPTRGRADIILHKTENHIIDSIFMRKY"
     gene            7655..8260
                     /locus_tag="IXT20_002995"
     CDS             7655..8260
                     /locus_tag="IXT20_002995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAQ4861353.1"
                     /translation="MTNHYYSERPDTAHDFEQWAFELKGRTFQFVTDSGVFSRETVDY
                     GSRVLIDAFDWEELPDEGKILDVGCGYGPIGLALAFATQRFVEMVDINERAVSLAQGN
                     AKRNQIEHVDIHQSNIYEAVHEETYAAIVSNPPIRAGKKVVHEILTGAYPRLKKGGTL
                     TIVIQKKQGAPSAQKKMEETFGNAEIVTKDKGYYIIKSVKE"
     gene            8568..9584
                     /locus_tag="IXT20_002996"
     CDS             8568..9584
                     /locus_tag="IXT20_002996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAQ4861354.1"
                     /translation="MKLTIRDIAEMAGVSVTTVSQILNNKGSRFSDKTRKKVLSIVNE
                     HHYKPDYFASNVINRHSKTIGMIVPDVTDFFFSKLIEGVESYLNPLGYVIMLCNSRHS
                     QENEIKYLQELSHRSVDGILLATPNILPEKYALDSGFYQKMPIILIDRGLNRRDNGRL
                     IVKEYEGAYQAVSLFIENGHTKIGMLKETTGYYQLEERFNGYRHALKDHGLPFNGKFV
                     EHGELTVQGGYTASKKLLKHKDITAIFCGNDAMAIGCYQAIDELGKKIPEDISVIGFD
                     GLKLSEYMIPQLTTVQQPSFDIGFYAARFLIDTIEFPQRKVPNKVFETKLIIRESVKV
                     LTKG"
     gene            9725..11026
                     /locus_tag="IXT20_002997"
     CDS             9725..11026
                     /locus_tag="IXT20_002997"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HAQ4861355.1"
                     /translation="MRMVDLIEKKRDGNELSKEEIEYIVTNYTNGKIPDYQVSALLMA
                     IFYQDMTNEEITNLTLAIANSGDVIDLSSLEGIKVDKHSTGGVGDTTTLILAPLVASV
                     GVTVAKMSGRGLGYTGGTLDKLEAIPGFQIELSDEEFVRIVNESKVAVIGQSGNLAPA
                     DKKLYALRDVTATVDSLPLIASSIMSKKIAAGADAIVLDVTTGDGAFMKNIEDARRLA
                     KTMTSIGKLANRETVAVISDMSEPLGEAIGNSLEVVEAIETLQGNGPEDLVEMCYALG
                     SQMVVLAGKAKTTDEARTLLQEALESGKALAKFKEMIQNQGGDPAIVEHPERILTARY
                     TMELPAKQSGVVSKIVANELGIAAMMLGAGRKTKEEDIDHAVGLKLHKKIGDTVTKGE
                     SLLTIYSNDEEITSVIELLYKNIEIGESAMKPTLIHDIITE"
     gene            11072..11734
                     /gene="deoC"
                     /locus_tag="IXT20_002998"
     CDS             11072..11734
                     /gene="deoC"
                     /locus_tag="IXT20_002998"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HAQ4861356.1"
                     /translation="MELNRMIDHTILKADASKEAVMQIIEEAKKYHFYSVCINPAWVA
                     LAKEQLQGEPVAVCTVIGFPLGANTPETKAFETTDAINNGADEVDMVINIGALKSKDD
                     QQVQKDIEAVVEAAKDRALVKVIIETSLLDREEIIRACEIAKAAGADFVKTSTGFSTG
                     GAKVEDVRLMRETVGPEMGVKASGGIHNEEEAMAMIEAGATRIGTSAGVAIVSGSTGE
                     GY"
     gene            11755..12153
                     /locus_tag="IXT20_002999"
     CDS             11755..12153
                     /locus_tag="IXT20_002999"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HAQ4861357.1"
                     /translation="MAELKQEWINTAIEALDKAYVPYSHFPVGACLVTESGKIYQGIN
                     IENASFGLTNCAERTAFFKAVSEGEKTFTHLVVAGHTPDPISPCGACRQVMAEFCSPD
                     MPVTLVGDNGVTKATTVRALLPYAFTEKDL"
     gene            12219..13301
                     /locus_tag="IXT20_003000"
     CDS             12219..13301
                     /locus_tag="IXT20_003000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HAQ4861358.1"
                     /translation="MKKAKLFGLGAVALAAGLFLGACGNNGSTDSSGGKSSSDTTTAA
                     LITDTGGVDDRSFNQSAWEGLEKWGKDQGLSRGNDGFQYFQSSNESDYIPNIDQALNA
                     GFKTIFGIGYKLKPAIQEQATNNTGTNFVIIDDVIDGLDNVVSATFKDNEASYLAGVA
                     AAYTTETNVVGFIGGVKGEVIDRFDAGFKAGVDAGAKELGKEIKVLNQYAGDFSAPDK
                     GRSIAQGMYAQNADIIFHASGGTGNGVFQEAKSLNESGDKKVWVIGVDRDQSDEGEYT
                     LNGEKKNFTLTSTLKAVGTVVEDLAQKSADGKFPGGEHTVYGLKEDGVGLTEGQLSDE
                     AKKAVDEAKEKIISGDVKVPETPEEN"
     gene            13513..15075
                     /locus_tag="IXT20_003001"
     CDS             13513..15075
                     /locus_tag="IXT20_003001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ4861359.1"
                     /translation="MVQENIVIEMQNITKEFGTFKANDNINLQVKAGEIHALLGENGA
                     GKSTLMNVLSGLLEPTSGKILMRGKEVKITSPTKANQLGIGMVHQHFMLVDAFTVTEN
                     IVLGSEPSRAGMLDHKKARKEIQKVSEQYGLSVNPDAYVRDISVGMEQRVEILKTLYR
                     GADVLIFDEPTAVLTPQEIDELIVIMKELVKEGKSIILITHKLDEIKAVADRCTVIRR
                     GKGIGTVNVKDVTSQQLADMMVGRTVSFKTMKKEAKPQEVVLSIENLVVKENRGLEAV
                     KNLNLEVRAGEVLGIAGIDGNGQSELIQALTGLRKAESGHIKLKGEDITNKKPRKITE
                     HGVGHVPEDRHKYGLVLDMTLSENIALQTYHQKPYSKNGMLNYSVMNEHARELIEEYD
                     VRTTNELVPAKALSGGNQQKAIIAREVDRDPDLLIVANPTRGLDVGAIEFIHKRLIEQ
                     RDKYKAVLLISFELEEILNVSDRIAVIHEGEIVGIVDPKETSENELGLLMAGYTLEEA
                     RKELEKGAGAHE"
     gene            15068..16201
                     /locus_tag="IXT20_003002"
     CDS             15068..16201
                     /locus_tag="IXT20_003002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ4861360.1"
                     /translation="MNDRSEKFRNLLVPVLSVVLGLILGAILMAAFGFQPIQGYSSML
                     DAALGSQRGIGETLRQATPLIFTALGFSVANSAGFFNIGLSGQALCGWIVSVWVALAF
                     PDLPKVVLLPLCVILGALAGAMAAAVPGLLRAFFGTSEVIVTIMMNYILLYVSTFALH
                     EVMPESYRSSLDSSNMISQNASLRLPWLTQAFGGSRVNSGLFLAIIALIFVWLLMKKT
                     TLGFEIRSVGLNPFASEYAGMSSKRTIVLSMVISGALAGLGGVVEGLGTYQNFFVQTT
                     SLSIGFDGMAVSLLGSGSAIGILLSAILFSILKIGGLGMQTGAGVPFEIVNVSIALII
                     FFVGINFLIRFVMAKVFQGKKQEEIVATIESKPTNQNHEGGEL"
     gene            16201..17157
                     /locus_tag="IXT20_003003"
     CDS             16201..17157
                     /locus_tag="IXT20_003003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ4861361.1"
                     /translation="MSTIELIALILTSTLVYSTPLILTSLGGTFSERSGIVNVGLEGI
                     MVMGAFSSIVFNLTMSPSLGSMTPWIAILVGGVVGILFSLIHAVATVSLRADHIISGT
                     VINLMAPALGVFLIKAWYGKGQTDNITQNLGYFSFPGLADIPVIGPIFFKNTFLPAYC
                     AILIAVLAWVIIFKTKFGLRLRSVGENPQAADTLGINVYAMRYSGVLISGLLGGMGGA
                     VFAQSISGNFSAGTIVGQGFISMAAMIFGKWNPLGAMGASLFFGFAQSLSIIGAQLPV
                     ISSIPPVLLQIAPYLLTIIVLVVFLGKASGPKANGKNYIKSK"
     gene            17256..18422
                     /gene="deoB"
                     /locus_tag="IXT20_003004"
     CDS             17256..18422
                     /gene="deoB"
                     /locus_tag="IXT20_003004"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HAQ4861362.1"
                     /translation="MFKRVHLIVMDSVGIGEAPDADKFGDVGSDTLGHIAKEAGLNVP
                     HLEQLGLGTIRPLEGVESVKDHQGYATKLEEVSVGKDTMTGHWEIMGLNIKTPFRVFP
                     NGFPDDLIKQIEEFSGRKVVCNKPYSGTAVIDDYGEHQMKTGDLIVYTSADPVLQIAA
                     HEEIIPLEELYRICQFVRDITKDDPYMIGRIIARPYVGEPGDFTRTSNRHDYALNPFG
                     HTVLDSLKEAGKDVIAVGKINDIFNGQGITESVRTKSNMDGVDQLLKVMKNDFSGISF
                     TNLVDFDALYGHRRDVVGYAHAIEEFDLRIPELLEAMEEDDLLMITADHGNDPTFTGT
                     DHTREYVPLLVYSKKMKEQGALPQGYYSDISATIAENFSVSSTENGESFLKLLK"
     gene            18446..19264
                     /locus_tag="IXT20_003005"
     CDS             18446..19264
                     /locus_tag="IXT20_003005"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HAQ4861363.1"
                     /translation="MTKLSEMLQETTQFILDKGVKEIDFGLILGSGLGELADEIEDAV
                     MISYDEIPFFPVSTVVGHAGKLVYGSLAGKKVLAMQGRFHYYEGHSMQTVTYPVRVMA
                     GLGAHSVIVTNACGGVNESFVPGDLMLITDHINFTGENPLIGPNEDAMGPRFPDMSQA
                     YTPDYQATAKAAAEKLGLDLKEGVYMGYSGPTYETPAEIRMSRTIGADAVGMSTVPEV
                     IVAAHSGLKVLGISCITNLAAGMQANLSHEEVVETTQRVKQSFKALVKETLTLL"
     gene            19281..19988
                     /gene="deoD"
                     /locus_tag="IXT20_003006"
     CDS             19281..19988
                     /gene="deoD"
                     /locus_tag="IXT20_003006"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HAQ4861364.1"
                     /translation="MSVHIEAKEGEIADKILLPGDPLRAKYIAETFLEDPVCYNQVRG
                     MLGFTGKYKGERISVQGTGMGMPSATIYAHELIQSYGVKKLIRVGTCGALSKDVHVRD
                     LVLAQGAATSSSMIEKNFQAFHFPPISDFNLLLKAYEIAKEKGYTVHVGNVLSEDSFY
                     KDDLTETFQLAELGVLGVEMEAAALYYLGAKYHVQTLSLMTVSDHLITGEETTAAERQ
                     STFNEMIEVGLETAIAE"
BASE COUNT         6453 a         3622 c         4333 g         5756 t
ORIGIN      
        1 ttacagaaat gtatacttca ataaatggtg aattatgtaa gattgataga gcttattctg
       61 gagaaattgt tattttgcaa aatgagtttt tgaagttaaa tagtgttctt ggagatacaa
      121 aactattgcc acagagaaaa aagattgaaa atccgcaccc tctactacaa acaactgttg
      181 aaccgagtaa acctgaacag agagaaatgt tgcttgatgc ccttttggaa atctcagata
      241 gtgatccgct tctacgatat tacgtggatt ctacgacaca tgaaattata ctttctttct
      301 tagggaaagt acaaatggaa gtgattagtg cactgttgca agaaaagtat catgtggaga
      361 tagaactaaa agagcctaca gtcatttata tggagagacc gttaaaaaat gcagaatata
      421 ccattcacat cgaagtgccg ccaaatcctt tctgggcttc cattggttta tctgtatcac
      481 cgcttccgtt gggaagtgga atgcagtatg agagctcggt ttctcttgga tacttaaatc
      541 aatcatttca aaatgcagtt atggaaggga tacgctatgg ttgcgaacaa ggattatatg
      601 gttggaatgt gacggattgt aaaatctgtt ttaagtacgg tttatactat agccctgtta
      661 gtactccagc agattttcgg atgcttactc ctattgtact ggagcaagcc tttagaaaag
      721 ctggaacaga attgttagag ccatatctta gttttaaagt ttatgcacca caggaatatc
      781 tttcacgggc atataacgat gctcccaaat attgtgcaaa tatcgtaaat actcaactga
      841 aaaataatga ggtcattatt attggagaaa ttcctgctcg atgtattcaa gattatcgca
      901 atgatttaac tttttttaca aatgggctta gtgtttgttt agcagagcta aaaggatatc
      961 aggttaccac tggcgaacct gtttgccaga cccgtcgtct aaatagtcgg atagataaag
     1021 taagatatat gttcaataaa ataacttagt gcgttttatg ttgttatata aatatggttt
     1081 cttattaaat aagatgaaat attctttaat atagatttga attaaagtgg aaaggaggag
     1141 attgttatta taaactacaa gtggatattg tgtcctattt gtggaaataa aacaagacta
     1201 cgaatacgag tggatactat acttaaaaat ttccctttat actgccccaa atgtaagaac
     1261 gaaactttaa ttaatgttca aaaaatgaat ataataacaa tcaaagagcc agacgccaag
     1321 acgcagagcc gataatttga gaaatgaaac tctcatctta tcggctcttt ttgtttatct
     1381 gaattttact gactagcctt caatatttct tgcttcattg ataccttttg ctagtgcttc
     1441 cattaaggat agttctttgt ctgtaaagct atccatgtat ttttctacct gtaaccttcg
     1501 agtgctttta atcaaattat ctgtaggtaa gaaaaattca tcaatagata catggagtaa
     1561 tgatacaaga tcatacagca cttgtgtact tgggtgttgt cctttgtttt ctatatttgt
     1621 taaataacgt gggtcaattt caatcattgt tcccacttgt tcacgagtga gaccccgttt
     1681 cattcgagcc tctttaatcg ctaaaccaac ggctctaaaa tcgtatttat cttctttttt
     1741 acgcatacta aatcacctct atacatttta ctgttcctat ataaccataa acaggtatag
     1801 aaaaacatgt tatatagttt atgagttcct atagaacact gtggaattta ttaatgcgat
     1861 gaatccaatt tcaaatagaa aggtcgctga ttttatttac ctatacaact ttataaaatt
     1921 tagtcatact cctagaaagt agataaaatt ggcgacttaa tggagggaaa agtcgccatg
     1981 agaaagataa tctgtcatct agttctctta attaagttct tatcaaaaaa acttatccac
     2041 ctgtactgga tagatatgac catcaatgtg gttttgcaga ttacatagat agaaaaatca
     2101 aagcatatga acagagaaaa caatctgttt atgtgctttt ttggtcactt aataaatatc
     2161 ttttaggatt tctcttcatt tatgcaacaa atcccccgtt cgtgtcgggg tttagtatga
     2221 agggagataa agaagcacat gccactccct ttcacgacag aaagggggtg aaaagcatga
     2281 aaccatcttc ttttcaagaa accatagaaa atcagtttga ctatatctgc aaacaagtta
     2341 ttgaagatga acgaaaagat tacttcaaac atctagacag actatcagca aaagaagtct
     2401 cgttttctga tatagataac taccttgtca actctttttc tactgttgat acgtacgtca
     2461 ctgatttcca actatttaaa ttatttggat taagaatcaa tgttgaaagt gatttattaa
     2521 gtgaagccct gcgtcatctt tcagagaaaa aacggaacat tattttactt cattacttta
     2581 tggatatgag tgatgtggag attgcggagt tactaaattt gaatcgttcg actgtttatc
     2641 gtcatcgaat aagtggacta gccatgatta aagaattcat gaaggagtgt aaagaatgaa
     2701 aacagcttat ccaagggttc ccttccccct cattgtaaag gcgacagatg gcgacgtgga
     2761 agcaattaat cagattgtta aacattatcg agggtatacc tcaaaacgtt cattacgtcg
     2821 catgacagac gaatatggaa attctcatat ggttattgat gaaaccctac gtggcagaat
     2881 ggaaacgaaa ctcatctcta agattttatc gttcgaaatt aaatagtccc attttttcgt
     2941 ggaagcgtgt cgaatcgtat ccacgcttcc cgatttggga ggtttgttat tccatcaaag
     3001 catattgtgt gttcagtagg ttttgatagg ctaacaagcc aaacgttctt tgacaaccaa
     3061 ataaaggcaa tcgaatacgt ttcggtaaga aaagagctga tgaactaaca cgccatgacc
     3121 tacaaaatta gtgagcgatt catgttagta attcgaaagt aatctttagc aggattgctg
     3181 ccatgacttt cttatcgtga taatgatact cccgtatagc catagtccga gcgttagaag
     3241 cgtcacaggc aatgggtacg gctacatgag aaccatgcag aagtgaaatt cctgtgagct
     3301 ttgctagagc tgttcgattg ctaagatatt cttctcttgt cagtgtatta ttataaaact
     3361 atttgatata attataggtg aatattggag ttttcctagc ctataattgg gaaaggagta
     3421 ataatgataa cggtcacaaa aaaggattta gttgagctag gatatggaac ttctttttca
     3481 gcagacatca taaaaaaagc aaaagaattg atgattacaa aagggcatat ctactatcaa
     3541 tctcgaaagc tagatagagt ccctcgtgaa gctgttgaag aaattctagg aattcggttt
     3601 tcaaatgaaa acaggtctga gtagtttttg cacagtaagg agtaaaaact atggttaaag
     3661 acccaataaa aaaagcaaag aatggaacct attattttcg tgcaaatcta ggttatgatt
     3721 ctaaagggaa aaaaattcaa aaatatcgta gtggattcac tactaaaaaa gaagcaagag
     3781 aagaatattc gaaactgcta ttgatgaaac cagaagaatt aagtgagaat aaggataaaa
     3841 tgacgtttaa acattatatc tttgaaattt tccttccatg gtataagaca caggtaaagc
     3901 tcagaacata tgaaaatcgc ttaccaacta ttaagaagca tttctcttat tttgataaaa
     3961 tggctgtttc cgatattgag cctattgatg tgcaaaattg gcaactaaaa ttatcaaaga
     4021 agtgtaagtc ttcttatgta agagctgttc aaggactatt ttcagttgct atggatcgag
     4081 caattgtcct tgggataacc accacaaatc catctaagat tattggcaat gtgaaaaagc
     4141 aaaagtctaa aattgaattt tggacaaaag aagaatttga aaaagtaatc tctctaattt
     4201 acaaagaaga ttactatcaa cactttttat ttatctctct gtggttttta tttatgactg
     4261 gaatgcgaat tggtgaagct actgccattc aatgggaaga tattgatttt gactcaggag
     4321 tactaaccat caacaaaaca ctctattaca agaatcaatc gaattataga tttgttgaac
     4381 caaaaacaaa ggcgagcgtt cgtcatattg tattagataa atgcacatta acttatttat
     4441 ccgaatggaa ggaagtacaa caaaatctaa ttcagacaga ctttgtaatg agctataacg
     4501 gtattcctac ccaaaaacat acactatcat atgctattga acgctatgct aagctggctg
     4561 gtatccatcg aatcagaatc catgggttac ggcattctca tgcttccttg ttaatcagta
     4621 tgggagaaaa tccacttatc attaaagatc gtctaggaca cgaagatatt gagacgactt
     4681 taggaacgta tggacatctt tatcctaaca gtaatttcga ggtagctaac aaattaaatg
     4741 gagccatttc ctttaaagaa gcaaaacaaa attatgatac ttcaccaaag aatcaattta
     4801 ctgtagacta cttgcgaaac aaagaaatga aaaaagtgca ataaaagtgc agtaaaaaga
     4861 taagaagagc cagaaaccct tggtataaga ggatttctag ctctcctaca actattccca
     4921 ctccactgtt gctggtggtt tagaagtgat gtcatacacg atacggttga cgtgggctac
     4981 ttcgtttacg atacgtacag agattttttg caagacttcc catgggatac gggcaaagtc
     5041 agcggtcatt ccgtcgattg aagttacagc acggataccg actgtgtagt cgtaagtacg
     5101 tccgtcaccc ataacaccga cagagcggat ccctggaaga acagtgaaat attgccagat
     5161 atcacggtca agtccagcag ctgcgatttc ttcacgcaag atagcatcag attcgcgtac
     5221 gatttctagc ttgtcttcag tgatttcgcc aagaacacga ataccaagac caggaccagg
     5281 gaatggctga cgccaaacga ttgaatcagg catgcctaac tgagtcccta attcacggac
     5341 ttcgtctttg aataatgtgt ttaatggttc gatcaattca aattgcatat cttctggcag
     5401 tccacctaca ttatggtgag atttgatcgt ctgtgctgtt tctgtaccag attcgataac
     5461 gtcagtatac aacgttcctt gtgctaggaa agagacgcct tcttcaccag caagttttgt
     5521 tgcttcgtca tcaaaaacat atacgaattc attcccaatg attttacgtt tttgttctgg
     5581 gtcagataca cctttcaact tgtctaagaa gcgatctctt gcatcgactt tgatgatgtt
     5641 caagccgaat tttccgccta gactttccat tacttgttcc gcttcgcctt tgcgtagcag
     5701 accgtggtca acaaagatag atgtcaattg gtcaccgatc gctttttgta aaagaacccc
     5761 aacgacacta gaatcaacgc cacctgaaag accaagcaag acttttttgt caccaacttg
     5821 ttcacggatt ttttctactt gcatatcgat aaagttatcc attgtccagt taccttcaca
     5881 gtgacaaaca tcaaaggcaa agtggcgcag caattcgatt ccgtattcgg aatgacgtac
     5941 ttctgcatgg aattggatac cataaaaatt acgtgttgtg tcttggatag aagcaatcgg
     6001 acaatctttg cttgttccga ctgtttcaaa gccttcagga actttagtta ccaagtcacc
     6061 atggctcatc catactgttt gttgttttgg tgtttcttta aacaagacag cttgatcgct
     6121 tgttacttct aacaaggctt tcccatattc gcggttttta gcaggctcta cggaaccgcc
     6181 aagtttgtac gtgatcaatt gcataccata gcaaatccct aatacaggaa tacctaaatt
     6241 gaagatctca ggatcgatcc caaatgcatt ctcatcatat acactgtttg gtccgccaga
     6301 aagaatgatt ccctttggat tcatcgcttt tacttcttct gcagtaatgc ggtggctcaa
     6361 taattcggaa aagactccaa gatcacgaat acgtcttgtg atcaattggt tatactgact
     6421 accgtagtct aatacgataa tcttttcaac gttcgtcata tcggcaacgt ttgtcaaatt
     6481 tattacccct atccctttaa attttatctc aaaatagtca aactatttta aaatgctaat
     6541 attttcgcat gaaaatcgaa tcgatgatat gattttcagt tttatggaga attatatcgg
     6601 ctcttcctcg tgtaggcaag atatattcct gtaaattttt caaattcaca tttttccata
     6661 catcttttgc cattttaaag gcttcttcac gatctccgat agcaaaacga taataataat
     6721 tctctggatt ttggaaagcc gtatccagta acgcaccaaa acgatccaaa taccattttt
     6781 cgatcagcac gggatcggca tcgacataga cagaaaaatc aaaaaagtcg ctgacgtaga
     6841 tttgctgatt cgcaggtaat tgaagcgtat tgatcccctc aacgatcaag atatcaggct
     6901 gctggatcaa ttcgtacttg tctggaatga tatcatacac atcatgtgag taaacaggta
     6961 cttttatctc ttcctgacca gattttacac ggttcaagaa atcgatcagc atctccatat
     7021 cataactttc aggaaatcct ttacggtcca tgattccacg ctcttttaat atacgatttg
     7081 gataaagaaa tccgtcagtc gtgatcagct gaacgtttct tcgtttgaag gtacgagaca
     7141 aaatcgtctg cagcagtctg gctgttgtac tttttccaac ggctacactt ccggcgatcc
     7201 cgataataaa aggcggtact tctacgtatt tatgaagaaa taaaccttta ctcaagaaca
     7261 aagattcaaa ttctttcata taaagataaa tcagatgagc aagaggtaca tagatatctc
     7321 gaacatcctg catggaaatg cggtcattca aacttttgat attatctagt tcatcttgtg
     7381 tcaaaggtgc ttgcccatta cgataaaatt ctcgccattc ttctcttgcc acacggtagt
     7441 agttcattgg atcattcata ttagcctcct gcgttctccc atcaatggaa ctattttaac
     7501 acaaaataaa cgcaaacccc accacttaag agagattgcc gatctttttg tataatcttt
     7561 ataatagaaa gaaacggagt cttattcttg acttgcgcac ggatacataa aaggatagac
     7621 tgttgtcatg aaagaaatga gaaggatgga agacatgaca aatcattact attctgaacg
     7681 cccagatacg gcgcacgatt ttgaacaatg ggcttttgag ttaaaaggca gaacctttca
     7741 atttgtaaca gattctggtg tattttcacg tgaaacagtg gattatggtt cacgtgtcct
     7801 gattgatgct ttcgattggg aagagttacc tgatgaagga aagatcttag atgtgggatg
     7861 cgggtatgga ccgattgggt tagctttggc atttgcgaca cagcggtttg ttgaaatggt
     7921 cgacattaat gaacgagcgg taagtctagc gcaaggaaac gcaaagcgta accaaatcga
     7981 acatgttgat atccatcaat cgaatatcta tgaagcagtt catgaagaaa cctatgcagc
     8041 tatcgtaagc aatccgccga tccgtgctgg aaaaaaagtt gtacatgaaa ttttgacagg
     8101 cgcatatcct cggttgaaga agggcggtac actgacaatt gtgatccaga agaaacaagg
     8161 agcgccgagt gcgcaaaaga aaatggaaga aacatttggt aatgcagaaa tcgtcacaaa
     8221 agataaagga tattatatca tcaaaagtgt gaaagaataa ctagctgaaa aatagtggag
     8281 cgacgaaaga gtggaactgg gcgtgcatgt acgggtacgt atcagttctt tttttgttga
     8341 gaagttttta aaaacaaaga actatttttt cttcaaaaag aaatagtagg aacagagtcg
     8401 ttgtttttta cgataaaata aataagatac agaacttgta tagaaggctg ccaagtcggt
     8461 agataaagga ggtttttatc aaaaaacata ctttcctttt ttctctattc ttttcttggg
     8521 ggttttggta cactgatagg gaatttagat agaagggagc caataggatg aagctgacca
     8581 tcagagatat cgcagaaatg gcaggcgtat cagtaacaac tgtctcacag attttgaata
     8641 acaaaggaag cagatttagt gataagacaa gaaaaaaagt gttgtcgatc gtgaatgaac
     8701 atcattataa gcccgattat ttcgcttcaa atgtgatcaa tcgacattca aagaccatcg
     8761 gaatgatcgt ccctgatgtc actgatttct tcttctccaa actgatcgaa ggtgtagaga
     8821 gctatttgaa tcctttgggc tatgtgatca tgttatgcaa ttctcgtcat agccaagaaa
     8881 atgagatcaa gtatttacaa gagctttccc accgctcagt ggacggaatc ttgctggcca
     8941 caccaaatat tttacctgag aaatatgcat tagacagcgg tttttatcaa aaaatgccaa
     9001 tcattttaat cgatcgcgga ttgaatcgtc gggataacgg acggcttatc gtaaaggaat
     9061 atgagggcgc ctatcaagct gtttcgctgt ttattgaaaa tggccatacg aagatcggca
     9121 tgctgaagga aaccactgga tactatcaat tagaagaacg tttcaacgga tatcgtcatg
     9181 cgctgaaaga tcatggcctg ccttttaatg ggaaatttgt tgaacatgga gagttgactg
     9241 tccaaggtgg atatactgca tcaaaaaaat tactcaaaca taaagatatc acggctattt
     9301 tttgcgggaa tgatgccatg gcgatcggat gctatcaggc gatcgatgaa ttgggcaaaa
     9361 aaatcccaga agatatctca gtcataggat tcgatggatt aaagctgtca gaatacatga
     9421 ttccgcaatt gacgacagtg cagcagccaa gttttgatat tggattttat gctgcacgtt
     9481 tcctgatcga tacgatcgaa tttcctcaaa gaaaagttcc gaacaaagtg ttcgaaacaa
     9541 aattaattat tcgtgagagc gtaaaagtat tgacaaaggg ctaactcggc ggtatattgc
     9601 ttatgtaagc atttacaaag gctagttagt gcttttttta tttttagtaa accagtttac
     9661 taaagtggtt tacctttcct aaaaataatg tataataccg aatgaagaca aggaggatca
     9721 ggcaatgcga atggtggatc tgattgaaaa aaagagggat ggaaacgagc tgtcgaaaga
     9781 ggaaatcgag tacatcgtca cgaactatac gaatggaaag attccagatt atcaagtcag
     9841 tgcattattg atggcaattt tttatcaaga catgacaaat gaagaaatca cgaacttaac
     9901 attggcgatt gctaattctg gtgatgtcat cgatttgagt tctcttgaag gaataaaggt
     9961 agataagcat tcgacaggtg gcgttggtga cacgaccaca ttgattttag cccccctagt
    10021 agctagtgtc ggagtgacag tggctaaaat gtctggacgc ggtctaggtt acacaggtgg
    10081 tacactagac aagctagaag caattcccgg atttcagatc gagctttcgg atgaagaatt
    10141 tgtacgcatt gttaatgaaa gtaaagtagc ggtaattggt caatcaggaa acttggcacc
    10201 ggcggacaaa aaattgtatg ccttgcgtga cgtcacagcg accgttgatt ctcttccgct
    10261 gattgccagt tcgattatga gtaaaaaaat cgcggcagga gcagatgcaa tcgttttgga
    10321 tgtgaccact ggagacggtg ctttcatgaa gaacatcgaa gatgcaagac gtctggcaaa
    10381 gaccatgacg agcattggga aactggctaa ccgagaaaca gtggcggtta tctcagatat
    10441 gtcagaacct cttggcgaag ctatcggaaa tagtctagaa gttgtcgagg caatcgaaac
    10501 attacaagga aatggtccag aagatctagt agaaatgtgc tacgccttag gaagccaaat
    10561 ggttgttctt gctggcaaag ctaagacaac cgatgaagca agaacgttgc ttcaagaagc
    10621 tctggaatca ggtaaagcgt tagcaaaatt caaagaaatg atccaaaatc aagggggaga
    10681 cccagcgatc gtggaacatc cagaacgaat tttgactgca cgctacacaa tggagcttcc
    10741 tgctaaacaa agcggagttg tgtcaaaaat cgtggcaaat gaattaggaa ttgctgcaat
    10801 gatgctaggt gctgggcgta aaaccaaaga agaagatatt gatcacgctg ttggattgaa
    10861 actgcataaa aaaattggtg acactgtgac aaaaggtgag tcactattga cgatctatag
    10921 taacgacgaa gaaatcactt ctgtcattga attattatat aaaaatattg aaattggtga
    10981 atcggcaatg aagccaacgt tgattcatga catcatcaca gaataaagga aaaaagcgga
    11041 ctttaccgtt ttaaagaaaa ggggaaaaga aatggaacta aaccgaatga tcgaccatac
    11101 tattttgaaa gcagacgcat ctaaagaagc tgtgatgcaa attatcgaag aagcaaaaaa
    11161 ataccatttt tattctgtct gcatcaatcc agcatgggtt gcgctagcga aagaacaatt
    11221 acaaggtgag ccagtagccg tatgtacagt aatcgggttt cctctaggag caaacacacc
    11281 cgagacaaaa gcatttgaaa caacagatgc catcaataac ggtgcagatg aagtcgatat
    11341 ggtcatcaat atcggtgctt tgaaatcaaa ggatgatcaa caagtccaaa aagatatcga
    11401 agcagttgtt gaagcagcaa aagatcgtgc gcttgtcaaa gttattattg aaacatcgtt
    11461 attagataga gaagaaatca tccgagcttg cgaaatagca aaagctgcag gcgctgattt
    11521 tgtcaaaaca tcaactggtt tctcaacagg cggagcaaag gtagaggatg tccgtttgat
    11581 gcgtgaaact gtaggaccag aaatgggtgt caaagcttcc ggcggtatcc ataacgaaga
    11641 agaagcaatg gcaatgatcg aagctggtgc tacaagaatc ggaacaagcg caggagtagc
    11701 aatcgtatct ggttcaactg gtgaaggcta ttaatttttt caaaggagaa gctcatggca
    11761 gaactcaaac aagaatggat caacacagct attgaagcgt tagataaagc ctatgttcct
    11821 tattcccatt ttccggtagg cgcttgtctc gtaacggaat caggcaagat ttatcaaggc
    11881 atcaacattg agaatgcatc atttggatta acgaattgtg ccgagcgcac agcttttttc
    11941 aaagcagtat ctgaaggaga aaaaacattt acgcatctag tcgtagcggg gcacaccccc
    12001 gatcctatct ctccttgtgg tgcatgtcga caagtaatgg cagaattctg ttcccctgat
    12061 atgccggtta cgctagtagg agacaatggt gtgacgaagg caacgaccgt tcgtgcactg
    12121 ctgccttatg catttactga aaaagatcta taactcatgt tcattcagtc agctgactgt
    12181 tgaaaaataa aacaattatt ttaggaggct ttacagaaat gaaaaaagca aaattatttg
    12241 gtttaggtgc tgtcgcacta gcagcagggt tatttttagg tgcatgcggc aacaacggct
    12301 ctactgattc atccggtggc aaaagctcat cagacacaac aactgcagca ttgatcacag
    12361 acacaggtgg tgtagacgac cgttcattca accagtcagc ttgggaagga ctagaaaaat
    12421 gggggaaaga tcaaggactt tcacgaggaa atgatggctt ccaatatttc caatcttcaa
    12481 atgaatcaga ttacatccca aatattgacc aagcattgaa tgctggattc aaaacgattt
    12541 ttggtattgg ttataaacta aaaccagcga tccaagaaca agcaacaaac aatacaggta
    12601 cgaactttgt tattatcgat gatgtcatcg atggattaga taacgttgta tctgcaactt
    12661 tcaaagacaa cgaagcttct tatcttgcag gtgttgcagc agcttatacg actgaaacaa
    12721 acgtagtcgg ctttatcggc ggtgtaaaag gtgaagtaat cgatcgtttt gatgctggat
    12781 tcaaagccgg tgttgatgca ggtgcgaaag aattaggaaa agaaatcaaa gtattgaatc
    12841 aatatgctgg tgacttctct gctccagata aaggacgttc aattgctcaa ggtatgtatg
    12901 cacaaaacgc agatatcatc ttccatgctt caggcggtac aggtaacggt gtattccaag
    12961 aagcaaaatc attgaacgaa tcaggcgaca aaaaagtttg ggttatcggt gttgaccgcg
    13021 accaatcaga cgaaggcgaa tacacattaa acggcgaaaa gaaaaacttc acattgacat
    13081 ctacactgaa agcagttgga acagtagtag aagatttggc acaaaaatca gcggacggta
    13141 aattccctgg cggcgaacac actgtttatg gattgaaaga agacggtgtt ggtctaacag
    13201 aaggacaact ttctgacgaa gcgaaaaaag cagttgatga agcaaaagaa aaaatcattt
    13261 caggtgacgt aaaagttcca gaaactcctg aagaaaacta aaaccatttc tatagccttg
    13321 ttagacacat gtttgacaag gctatggttt gattagaaca agaattgttc atttatccaa
    13381 gctgccttgt gacagtttgg ataagggaac aactccctga agtaatgttt gtttcaaaag
    13441 aagaacgctc actaagcgtg aatcttagtc aatcgttgcc ctagatagaa atcgttagga
    13501 tgtgaaaaaa aggtggttca agaaaatatt gtcatagaaa tgcagaatat taccaaagaa
    13561 tttggaacat tcaaggcaaa tgacaacatc aatttacaag taaaggcagg agaaattcat
    13621 gcgttgcttg gagaaaacgg tgctggcaaa tctacattga tgaacgtgct ttccggatta
    13681 ttagagccga catcagggaa aattttgatg cgtgggaaag aagtaaagat cacaagtccg
    13741 accaaagcca accaattagg gattgggatg gtccatcagc actttatgct tgttgatgcc
    13801 tttactgtaa cagaaaacat cgtgttggga agcgagccta gtcgcgcagg gatgcttgac
    13861 cataaaaaag cacgaaaaga gatccaaaaa gtttctgaac agtatggatt gtcagtcaac
    13921 ccagatgctt acgttcgtga tatttcagtt ggaatggaac aacgggtaga aattttaaaa
    13981 acactttacc gaggagcaga tgtactgatt tttgatgagc cgacagctgt attgacccct
    14041 caagaaattg atgaattaat cgtgatcatg aaggaattag tcaaagaagg caagtcgatc
    14101 attttgatta cgcataagtt agatgaaatc aaagcagtag ctgaccgctg tacagttatc
    14161 cgccgtggaa aaggaatcgg tacagtcaac gttaaagacg taacctcaca acaattagcc
    14221 gatatgatgg ttggaagaac ggtttcattc aaaacgatga aaaaagaagc gaagcctcaa
    14281 gaagtcgttt tgtctattga aaatctagtg gtgaaagaaa atcgtggatt agaagccgtg
    14341 aaaaacttga acttagaggt tcgtgctggc gaagtacttg gtatcgctgg aatcgatgga
    14401 aacgggcagt cggagttgat ccaagctttg actggtttgc gaaaggcaga aagcggacat
    14461 atcaagctaa aaggggaaga catcactaat aaaaaacccc gaaaaatcac tgaacatggt
    14521 gtaggacacg tgccagaaga ccgtcataaa tacgggttag tcctagatat gacattatct
    14581 gaaaatatcg cactacaaac ctatcatcaa aaaccttaca gtaaaaacgg catgctgaat
    14641 tattcagtaa tgaatgaaca tgccagagaa ttgatcgaag aatatgatgt tcgaacaacg
    14701 aatgaacttg ttcccgcaaa agctttatca ggcggaaatc agcaaaaagc aattatcgct
    14761 cgtgaagtcg accgagatcc tgatctgttg attgttgcaa atccaactcg tggactggat
    14821 gtaggtgcga tcgaatttat tcataaacgt ctgatcgaac aaagggacaa atacaaagca
    14881 gtgttactga tcagtttcga attagaagaa attttaaatg tttcggatcg tattgctgtt
    14941 atccatgaag gagaaattgt cggtatcgtt gatccgaaag aaacatctga aaatgaatta
    15001 ggtctattga tggcgggcta taccttagaa gaagcacgaa aagagttaga aaagggggct
    15061 ggcgcacatg aatgatcgca gcgaaaaatt ccgtaattta ttagtgcctg tactttcagt
    15121 tgttttagga ttgatcttag gcgcgatatt gatggctgct tttggttttc agcccatcca
    15181 aggttattct tctatgctag atgcagccct tggttcgcaa agagggatag gtgaaacgtt
    15241 aaggcaagca acgccgttga tttttacagc attaggtttt tctgtagcta actcagctgg
    15301 gtttttcaat attgggcttt ctggacaagc actttgcggg tggatcgtca gtgtctgggt
    15361 ggcacttgcg tttccagatc tgccaaaagt tgttttgctt ccgttatgcg tgatcttagg
    15421 tgccttagct ggtgcgatgg cagcggctgt tccaggttta cttcgagcat tttttggaac
    15481 aagtgaagtg atcgtaacaa tcatgatgaa ctatatctta ttatacgtaa gcacatttgc
    15541 tttacatgaa gtgatgcctg aaagctatcg ctcaagtttg gactcttcca atatgatcag
    15601 tcaaaatgcc tcactgcggt taccatggtt aacacaagct tttggcggct ctcgcgtaaa
    15661 ctcaggatta tttttagcaa tcatcgcgct tattttcgtt tggctgctga tgaagaaaac
    15721 gactttagga tttgaaatcc gttctgttgg attgaatcct tttgcttctg agtatgcagg
    15781 aatgagtagt aaacgtacga tcgttttgtc gatggtcatt tctggcgctt tggcagggct
    15841 tggcggtgtg gttgaaggat taggaacgta tcaaaacttc tttgttcaaa cgacttcgct
    15901 atctattgga tttgacggaa tggctgtttc cttgcttggt tctggatcag caatcggtat
    15961 tcttttatca gcgatcttgt tcagtatttt gaagatcggt ggacttggca tgcagacagg
    16021 agcaggtgtt ccgtttgaga tcgtcaatgt atcgattgct ttgattatct tctttgtcgg
    16081 gatcaacttc ctgattcgtt tcgtcatggc taaagtcttc caagggaaaa aacaagaaga
    16141 gatcgttgcg acaatagaat cgaaaccaac gaatcaaaac catgaagggg gcgaactata
    16201 atgtcgacta tcgaattaat tgccttgatc cttacttcta cactggttta ttcaacaccg
    16261 ctaattctaa cgtcactagg cggaactttt tctgaacgaa gcgggatcgt aaacgtaggg
    16321 ctagagggaa tcatggtcat gggtgctttt agttccatcg ttttcaactt gacgatgagt
    16381 ccatcacttg gttctatgac accatggatc gctattttag tgggtggcgt cgtaggtatc
    16441 ctattttctc tgattcacgc agtagcaaca gtcagtttgc gtgctgacca tattatcagc
    16501 gggacagtaa ttaatctgat ggcacctgct ttaggtgttt tcttgatcaa agcatggtat
    16561 ggtaaaggtc aaactgataa catcacgcag aatctggggt atttttcttt cccaggatta
    16621 gcagatattc cggtcattgg accaattttc tttaaaaata cattcttacc tgcttattgt
    16681 gctattttga ttgctgtcct agcttgggtg atcatcttca agacgaaatt cggcttgcgc
    16741 ctccgttcag ttggtgaaaa tcctcaagct gccgacacat taggtatcaa tgtatatgct
    16801 atgcgttatt caggcgtact gatttctggc ttgttaggcg gtatgggcgg tgctgttttt
    16861 gctcagtcga tttccggtaa cttttcagct ggtacgatcg ttggacaagg gttcatctcc
    16921 atggctgcta tgatctttgg taagtggaat cctttagggg caatgggtgc ctcattgttc
    16981 tttggttttg ctcaaagctt gagtatcatt ggcgcacaat taccagttat ttcttcgatt
    17041 ccgccagtat tgctgcaaat tgctccatat ttgctgacaa tcattgtgct ggttgttttc
    17101 ttagggaaag cttctggtcc aaaagcaaac gggaagaact atatcaaatc aaaataatct
    17161 tttagcgaat aaaaagggtg ggttacggga agtgtttttc gtctcgccct ttttgtttta
    17221 aaggcaataa aaataaaaag aaagaaggaa taatcatgtt taaacgcgta catctgatcg
    17281 tcatggattc agtaggaatt ggagaagcac cagatgccga taagttcggt gatgttggtt
    17341 ctgatacatt aggacacatt gcgaaagaag caggattgaa cgttccacat ttagagcagc
    17401 ttggtttagg aacgatccgt cctcttgaag gtgtggaatc agtgaaagat catcaaggct
    17461 atgcgacgaa attagaagaa gtttctgtag ggaaagatac gatgacaggc cactgggaaa
    17521 tcatgggatt gaatatcaaa actccatttc gtgtgtttcc taatggattc cctgatgatc
    17581 tgatcaaaca aatcgaagaa ttttctggcc gtaaagtcgt atgtaacaaa ccttatagcg
    17641 gaacagctgt tattgacgat tacggtgaac accaaatgaa aacaggcgat ttaattgtat
    17701 atacttctgc cgatcctgtc cttcagatcg cggcacatga agaaatcatt cctttagaag
    17761 aattatatcg gatttgccaa ttcgttcgtg atataacaaa agatgatccg tatatgattg
    17821 gccgaatcat tgcacgtccg tacgtgggtg aaccaggaga cttcacacga acaagcaatc
    17881 gccatgatta tgcgttgaat ccattcggac acacagtcct tgattctttg aaagaagcag
    17941 gaaaggacgt gattgctgtt ggaaagatca acgatatttt caacggacaa ggaatcactg
    18001 aatctgtccg cacgaaaagc aatatggatg gcgttgacca gctgctgaaa gttatgaaaa
    18061 acgatttttc aggaatcagc tttaccaatt tagtagattt tgatgcatta tacggtcatc
    18121 gtcgggatgt agtcggctat gcgcatgcga ttgaagaatt tgacttacga attccagaat
    18181 tgcttgaagc gatggaagaa gacgacttgc taatgattac agccgatcac ggaaacgatc
    18241 cgacgtttac tggtaccgat catacgcgag aatatgtacc gttattggtt tacagtaaaa
    18301 aaatgaaaga acaaggggca ttgcctcaag gatattattc agatatttca gcaacgattg
    18361 ctgagaactt tagtgtttct tcgactgaaa atggcgaaag cttcttgaaa ttattaaaat
    18421 aatcttttta aataggagaa agcaaatgac caagttaagc gagatgttac aagaaacgac
    18481 ccagtttatc cttgataaag gagtaaaaga aattgatttt ggtttgattc taggttcagg
    18541 attaggtgaa ttagcagatg aaatcgaaga tgctgtaatg atttcttatg atgagatccc
    18601 gttctttcct gtttcgacag tagttgggca tgcaggaaag ctagtttatg ggtctctggc
    18661 aggcaagaaa gtgttagcca tgcaagggag attccactac tatgaaggtc attccatgca
    18721 aacagtgacc tatccagtac gtgtgatggc cggactaggg gcccattcag tcattgtgac
    18781 taatgcatgt ggtggtgtca acgaatcatt tgtaccagga gatttgatgc tgattaccga
    18841 tcatatcaat tttactggtg aaaatccttt gatcggtccg aatgaagatg caatgggccc
    18901 tagatttcca gatatgagtc aagcttatac gcctgactat caagcaacag caaaagcagc
    18961 agcagaaaaa ctaggtttgg atcttaaaga aggcgtatat atgggctatt ctggaccaac
    19021 ttatgaaacg cctgcggaga tccgaatgtc tcggacaata ggagccgatg cagtagggat
    19081 gtcgactgtt ccagaagtca tcgtagcagc tcacagcgga ttaaaagtgt taggaatctc
    19141 ttgtattacc aacttagcag caggtatgca agcgaatctg agccatgaag aagttgtaga
    19201 aacaactcag agagtcaaac aatcttttaa agcattggtt aaagaaacat tgacattatt
    19261 gtaataggag gaacaataaa atgagtgtgc atattgaagc gaaagaaggc gagatcgccg
    19321 ataagatttt acttccggga gatcctttgc gagctaaata tattgcggag acttttttgg
    19381 aagatccagt ttgttacaat caggtacgcg gtatgttagg ctttaccggc aaatataaag
    19441 gagaacgtat ttctgttcaa ggaacaggaa tgggaatgcc atcagccacc atctatgccc
    19501 atgaattgat ccagtcgtat ggtgtaaaga aattgattcg agtaggtaca tgcggtgcat
    19561 tgtcaaaaga cgtccatgtt cgtgatttag tactagcaca aggtgcagcg accagttctt
    19621 cgatgatcga aaaaaatttc caagcctttc attttcctcc aatcagtgat ttcaatctat
    19681 tactgaaagc atatgagata gctaaagaaa agggctatac ggttcatgta ggaaatgttc
    19741 tttcggaaga ttctttctac aaagatgatt tgacagagac gttccaatta gctgaacttg
    19801 gcgttttagg agtggaaatg gaagcagcgg ctctttatta cttaggcgcg aaataccatg
    19861 tgcagacatt aagtttgatg accgttagcg accatttgat caccggtgaa gaaacaacag
    19921 ctgcagaacg tcaatctacg ttcaatgaaa tgatcgaagt aggattggaa acagcgatag
    19981 cagaataaga aggaaatgaa aaagctgccc tgcattaagt aataagaaca tccaagcaaa
    20041 aatagctcct catgtttttg acttggctgg acttattatt gcaaatgtaa gcttttgaac
    20101 accgtttacc aagaggttgt gaatctgccg tttaactctg agtcacaagg aacaatttca
    20161 aaaa
//
LOCUS       DACGLE010000215        11347 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0534
            SAMEA3304449-rid9560453.guided.086, whole genome shotgun sequence.
ACCESSION   DACGLE010000215 DACGLE010000000
VERSION     DACGLE010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304449
            Sequence Read Archive: ERR985064
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11347)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11347)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702225.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702225.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 20:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,045
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 27 of 67
            Pseudo Genes (incomplete)         :: 25 of 67
            Pseudo Genes (internal stop)      :: 19 of 67
            Pseudo Genes (multiple problems)  :: 4 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11347
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304449-rid9560453.guided.086"
                     /strain="VREN0534"
                     /db_xref="taxon:1352"
     gene            216..1127
                     /locus_tag="IXT20_003007"
     CDS             216..1127
                     /locus_tag="IXT20_003007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala carboxypeptidase VanY-A"
                     /protein_id="HAQ4861365.1"
                     /translation="MKKLFFLLLLLFLIYLGYDYVNEALFSQEKVEFQNYDQNPKEHL
                     ENSGTSENTQEKTITEEQVYQGNLLLINSKYPVRQESVKSDIVNLSKHDELINGYGLL
                     DSNIYMSKEIAQKFSEMVNDAVKGGVSHFIINSGYRDFDEQSVLYQEMGAEYALPAGY
                     SEHNSGLSLDVGSSLTKMERAPEGKWIEENAWKYGFILRYPEDKTELTGIQYEPWHIR
                     YVGLPHSAIMKEKNFVLEEYMDYLKEEKTISVSVNGEKYEIFYYPVTKNTTIHVPTNL
                     RYEISGNNIDGVIVTVFPGSTHTNSRR"
     gene            1280..1765
                     /gene="vanZ"
                     /locus_tag="IXT20_003008"
     CDS             1280..1765
                     /gene="vanZ"
                     /locus_tag="IXT20_003008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000516404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycopeptide resistance protein VanZ-A"
                     /protein_id="HAQ4861366.1"
                     /translation="MGKILSRGLLALYLVTLIWLVLFKLQYNILSVFNYHQRSLNLTP
                     FTATGNFREMIDNVIIFIPFGLLLNVNFKEIGFLPKFAFVLVLSLTFEIIQFIFAIGA
                     TDITDVITNTVGGFLGLKLYGLSNKHMNQKKLDRVIIFVGILLLVLLLVYRTHLRINY
                     V"
     gene            complement(2052..2381)
                     /locus_tag="IXT20_003009"
     CDS             complement(2052..2381)
                     /locus_tag="IXT20_003009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000754864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ4861367.1"
                     /translation="MKKVCQVTVVHKEKVNGIKNKLDQKDFSNLLVLGKCFSDFSRIK
                     IFYALETYKEMCVCDLAEILTASVATTSHHLRFLKKHGMAKSRQDGKVVYYSLANEDI
                     LSAIRVF"
     gene            2686..3015
                     /locus_tag="IXT20_003010"
     CDS             2686..3015
                     /locus_tag="IXT20_003010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007723369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAQ4861368.1"
                     /translation="MITKTRKQGNSIMLTVPKEFDVPNGVEVEAKLVENGILYEFVEP
                     KKEFFDFSEDILADILSEGYSKQEILKEFRNRKNALTSAFKSIAEDTVANSKPMTKEE
                     LAAEIGL"
     gene            3005..3292
                     /locus_tag="IXT20_003011"
     CDS             3005..3292
                     /locus_tag="IXT20_003011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000312842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HAQ4861369.1"
                     /translation="MAYEILPSKHVIKYLKKLKEKTLKEQFLTIIYDEIAVRPHSGEQ
                     KTGDLSGIWAIGFKYAGTTYRVAYEIKDNAVIPILLCGTHENFYEQLKKIR"
     gene            complement(3571..4134)
                     /locus_tag="IXT20_003012"
     CDS             complement(3571..4134)
                     /locus_tag="IXT20_003012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000017403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAQ4861370.1"
                     /translation="MSGYIYGYARVSTRSQELNRQLDLLNEQNCNEILTEKMTGTKAV
                     RPQLNRLKDKIRPGDTVIVESFSRLGRSTKDLIDLVTYFEEHHVKLVSLKENFDTSTP
                     HGRLMMTVFQAFSQFERDLIVERTKEGLKSARARGRKGGRPRVNQKDVDRAVKLYKSQ
                     VYSVKEITEMTGISKATLYRYLKDNRE"
     gene            complement(4327..4899)
                     /locus_tag="IXT20_003013"
     CDS             complement(4327..4899)
                     /locus_tag="IXT20_003013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIIA family hydrolase"
                     /protein_id="HAQ4861371.1"
                     /translation="MNKAIFWDLLGTLGGDSRTLINQNFNFFKDTIPALKKAAQSHYL
                     NIIITNQSHISHGRITMAEYTDALESLINELEKNDIQITDVYTCPHARKDNCDCKKPQ
                     PTFVNEALKKYDLNPSKCFVVGDSGKNDMMLAKNAGINSVLLLTGEGIDSLTKYRHLW
                     SETKPTFIAENSLDAINKIHMYADGDSSDS"
     gene            5687..6262
                     /locus_tag="IXT20_003014"
     CDS             5687..6262
                     /locus_tag="IXT20_003014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4861372.1"
                     /translation="MKNKFKYLFTILALLPITTSTLITPTIAKASEKQEDSVSYEIVK
                     NDLYIQFEEELAELIASNQSTVSVPDLFSTQSVLSTSSQDNSISEEQFFSLVEKYDGA
                     ELTAVVNSQDAFTTKFVNGGVNSYATSYRGLNALRSELKYNAGALKVGGTLVGTAGGL
                     LGVLLGMVTGDYLSGHCDDAAAGVSAFIQAG"
     gene            complement(6445..7197)
                     /locus_tag="IXT20_003015"
     CDS             complement(6445..7197)
                     /locus_tag="IXT20_003015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAQ4861373.1"
                     /translation="MMLNEQTEVKLAELKLSGMLESYREQMTNKEYQSMSFEDRFNLM
                     VDLEHSRRKNNKLQRLIKSANFRNASASIEDIEYHPDRNLDKNLILKLASGTYLEEHH
                     NIILMGASGNGKTYIANAFGIQACRQYYNVKYIRLPELLDELTISKNQADGSFRKIIN
                     RYKKVQLLIIDEWLLTNLSLENSTFLLEIIESRLKTASTIFCSQFHPEGWYEKLGNPQ
                     IADAILDRIIHDSYQILVDGKVSMRERYGIRN"
     gene            complement(7197..8738)
                     /locus_tag="IXT20_003016"
     CDS             complement(7197..8738)
                     /locus_tag="IXT20_003016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010632830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HAQ4861374.1"
                     /translation="MFRYRRILEMHSEGISIRSIAASTGNSRQKITEVIRKAEEKGIA
                     RALSEEMTDQWLGEFLFPEKQLEANGRHLPDFDKIHKELAKPNVTLTLLHQEYAVECR
                     MSGKIPYAYRTFCDYYNAFARKHKATMRIRRKPGEIMEVDWAGSPAYVIDRDTGEKVK
                     AYVFVATLPCSQLSYAEAFLTMKSVAWITAHNHAFEYFGGSTDIIVPDNLKTGVKKNE
                     GIDVILNESYRELGEHYGSIILPARVSTPKDKPSVEGSVGVISTWIIAALRNTHCFTI
                     EDLNREIRMKLNEFNERPFTKKPGSRIRAFEEEEKFALNPLPLAPYKMSVWKKAKVQL
                     NYHVSVESMFYSVPYEYIQQNIDIRMTENLIELYFNSMRIATHKRLYGKFGQYSTVRD
                     HMPDNHKMYVDHTPESSMEWAETIGSSTVKVVQHLLDRYQNEKQALKSIMSLKSFGKK
                     YSKNEIETACSTALNLASLPSVKIIQTILKGNKQKRATSQSHIEDTNIEKHGFTRGAS
                     YFGGK"
     gene            9652..10116
                     /locus_tag="IXT20_003017"
     CDS             9652..10116
                     /locus_tag="IXT20_003017"
                     /inference="COORDINATES: protein motif:HMM:NF037267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4861375.1"
                     /translation="MRQVLLVVTLCLSFFLFEVNTVEAYVANNGVYYNLIQGGTQEFM
                     EVDEEGNPILIRVEEVPGLSLFSVNNGNYKISGSKFGMWKANYYVTVSNNQFIRAYSP
                     SAVAVTGSFTSTRLSLVSSKKASYTLEWKAGILSSNSYLDATLTDNSINITY"
     gene            complement(10113..10637)
                     /locus_tag="IXT20_003018"
     CDS             complement(10113..10637)
                     /locus_tag="IXT20_003018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007722706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ4861376.1"
                     /translation="MQNDIRGKIKKSRHIKGISQSEVAKYLNISRQSISKWENDTSSP
                     DINNLTLLSSLYEISIDDLLKEKKEVKKNIKENSIEIIESKNKLIYLKNNTNRDEGLV
                     LLLISLIGCLISPLGLIIAPVVLKRNKATNTFYKGVIAASVICFTVSLFVSYGHFSNI
                     FHWGGSTNVEYIEN"
     gene            complement(10687..>11347)
                     /locus_tag="IXT20_003019"
     CDS             complement(10687..>11347)
                     /locus_tag="IXT20_003019"
                     /inference="COORDINATES: protein motif:HMM:NF015755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAQ4861377.1"
                     /translation="LFDSRFKTLQQNRYVDIIGYDTYDKINHAYAFGGVAMTINTIQA
                     YLDIPIDFFVTLNMQGLEQIVDSIGGITLTPTATFTQDSYSFYQGEIINLDGKASLAY
                     SRNRHADDSGDYGRQARQREIIMAIIEKGTKVTTLATKIPSLIKSLQGNMTTNLSVDD
                     MWTIINKYSTALGNIEQVQLKGEGQLLDDGIYYEMINEESLEEVQNILQKQLDYHLNI
                     D"
BASE COUNT         3625 a         1885 c         1985 g         3852 t
ORIGIN      
        1 cttatttgat agtagattta aaactttgca acagaaccgt atcacttatg aaaattcatc
       61 tacattggtg ataatagtaa atccagtagg gcgaaataat tgactgtaat ttacggggca
      121 aaacggcaca atctcaaacg agattgtgcc gtttaagggg aagattctag aaatatttca
      181 tacttccaac tatatagtta aggaggagac tgaaaatgaa gaagttgttt tttttattgt
      241 tattgttatt cttaatatac ttaggttatg actacgttaa tgaagcactg ttttctcagg
      301 aaaaagtcga atttcaaaat tatgatcaaa atcccaaaga acatttagaa aatagtggga
      361 cttctgaaaa tacccaagag aaaacaatta cagaagaaca ggtttatcaa ggaaatctgc
      421 tattaatcaa tagtaaatat cctgttcgcc aagaaagtgt gaagtcagat atcgtgaatt
      481 tatctaaaca tgacgaatta ataaatggat acgggttgct tgatagtaat atttatatgt
      541 caaaagaaat agcacaaaaa ttttcagaga tggtcaatga tgctgtaaag ggtggcgtta
      601 gtcattttat tattaatagt ggctatcgag actttgatga gcaaagtgtg ctttaccaag
      661 aaatgggggc tgagtatgcc ttaccagcag gttatagtga gcataattca ggtttatcac
      721 tagatgtagg atcaagcttg acgaaaatgg aacgagcccc tgaaggaaag tggatagaag
      781 aaaatgcttg gaaatacggg ttcattttac gttatccaga ggacaaaaca gagttaacag
      841 gaattcaata tgaaccatgg catattcgct atgttggttt accacatagt gcgattatga
      901 aagaaaagaa tttcgttctc gaggaatata tggattacct aaaagaagaa aaaaccattt
      961 ctgttagtgt aaatggggaa aaatatgaga tcttttatta tcctgttact aaaaatacca
     1021 ccattcatgt gccgactaat cttcgttatg agatatcagg aaacaatata gacggtgtaa
     1081 ttgtgacagt gtttcccgga tcaacacata ctaattcaag gaggtaagga tggcggaatg
     1141 aaaccaacga aattaatgaa cagcattatt gtactagcac ttttggggta acgttagctt
     1201 tttaatttaa aacccacgtt aactaggaca ttgctatact aatgatacaa cttaaacaaa
     1261 agaattagag gaaattatat tgggaaaaat attatctaga ggattgctag ctttatattt
     1321 agtgacacta atctggttag tgttattcaa attacaatac aatattttat cagtatttaa
     1381 ttatcatcaa agaagtctta acttgactcc atttactgct actgggaatt tcagagagat
     1441 gatagataat gttataatct ttattccatt tggcttgctt ttgaatgtca attttaaaga
     1501 aatcggattt ttacctaagt ttgcttttgt actggtttta agtcttactt ttgaaataat
     1561 tcaatttatc ttcgctattg gagcgacaga cataacagat gtaattacaa atactgttgg
     1621 aggctttctt ggactgaaat tatatggttt aagcaataag catatgaatc aaaaaaaatt
     1681 agacagagtt attatttttg taggtatact tttgctcgta ttattgctcg tttaccgtac
     1741 ccatttaaga ataaattacg tgtaagatgt ctaaatcaag caatctgatc tttcatacac
     1801 ataaagatat tgaatgaatt ggattagatg gaaaacggga tgtggggaaa ctcgcccgta
     1861 ggtgtgaagt gaggggaaaa ccggtgataa agtaaaaagc ttacctaaca ctatagtaac
     1921 aaagaaagcc caattatcaa ttttagtgct gaggaattgg tctctttaat aaatttcctt
     1981 aacgttgtaa atccgcattt tcctgacggt acccccaaac taagatttca tagatagatt
     2041 ttctgataac tttaaaaaac acgaatagcg gaaaggatgt cttcattcgc taaagagtaa
     2101 taaactactt ttccatcttg acgtgatttt gccattccat gtttttttaa aaaacgtaaa
     2161 tggtgtgacg ttgtagcgac actagcagta agtatttctg ctaaatcaca gacgcacatt
     2221 tccttatagg tttctaaagc atagaaaatt ttaattctag aaaaatcgct gaaacatttc
     2281 cctaacacaa gtaagtttga aaaatctttt tgatccaatt tattttttat tccatttact
     2341 ttttctttat gtacgacagt cacttggcaa acttttttca ttcatctcac ctcacattca
     2401 aacgattatt tgaatgaagt atacctattt gatcactctt ttgtcaatct accttaattc
     2461 caatacacta ttataagcaa tttcatgtat tgaagtaaac ttacttgcaa gtgcgttttt
     2521 gattccaagt taaaaacata ttattattct acataatttc ctaatcaaat aaaactcctt
     2581 tgaaaattgt tctaaaggag ttttattatt ttgtatttct actaaaaata gcgtacaatg
     2641 taattactaa gtaattacga aatgtaaaat aatggaggaa ataatatgat tacaaaaaca
     2701 agaaaacaag gaaattctat tatgttaacg gtccctaaag aattcgatgt gcccaatggt
     2761 gttgaagtag aagctaagct tgtagagaat ggtatccttt atgagtttgt agagcctaaa
     2821 aaagaatttt ttgattttag tgaagatatt ttggctgata ttctttcaga aggttatagc
     2881 aaacaagaga tcttaaaaga atttagaaat agaaaaaatg cgttaacttc tgcatttaaa
     2941 tctattgctg aagatactgt tgcaaattct aaaccgatga caaaagagga gttggcagca
     3001 gaaattggct tatgagattc taccttctaa acacgtaata aagtatctaa aaaaattaaa
     3061 agagaaaaca ttaaaagaac aatttttaac gataatttat gatgagatag cagttcgtcc
     3121 tcattcggga gaacaaaaaa cgggtgattt atcaggaatt tgggctattg gattcaagta
     3181 tgcaggtacg acatatcgcg ttgcatatga aattaaagat aatgcagtga tacctatctt
     3241 actatgtgga acgcatgaaa atttttatga acaattaaaa aagattaggt aagaacccaa
     3301 tgtttcaact tgagaggata gacgacactt taaaagataa tttttaacag ctgagagttg
     3361 taatacccta taattttaaa ccactaacct atatatttat gaaaaataga aaaaattaaa
     3421 acgcatttta agcctctttc aaattaaaag cttcatcctt atgcacaaag agggcttaaa
     3481 atgcgttatt ttatattgtt ttttagtaat aataaaaaaa tcactctaat tctcccataa
     3541 atattataga ttcctaaaga ttaaaataga ttattcccta ttgtctttta agtagcggta
     3601 gagtgttgcc ttgctaatac cagtcatttc cgtaatttct tttacgctat aaacctgact
     3661 tttatacaat tttactgctc gatcaacatc tttctggttc actcgtggac ggccaccttt
     3721 acgacctctc gcacgtgcac ttttcaaacc ttctttggta cgttcaacaa ttaaatcacg
     3781 ttcgaactga ctaaatgctt ggaagacagt catcattaag cgtccgtgag gcgtgctggt
     3841 atcgaagttt tctttcaagc tcaccagctt aacatggtgt tcttcaaaat aggtaacaag
     3901 gtcaatcaaa tcctttgtac tacgacccaa tcgagaaaaa ctttctacaa ttaccgtatc
     3961 tcccggacga attttatctt ttaaacgatt tagttgggga cgaaccgctt tggttcccgt
     4021 cattttctca gttaggattt cattacaatt ttgttcattg agtaagtcta attgacgatt
     4081 tagttcttgt gaacgtgtac tcactcgggc ataaccataa atgtatccac tcatgctttt
     4141 cttcctttcg tactttaatt ctggcttgat tgtatcataa aagggagtta tagatacata
     4201 gttaatgcaa caagttatga tacaaaatat gacctctttt atttaataaa agaggtcatt
     4261 tcattttgtc tcataatcga ttgtttttga tacggttttt agtaataaat ggagactctt
     4321 ctggtatcat gaatcagaag agtctccatc tgcatacata tgtattttat ttatagcatc
     4381 taaactgttt tcagcaataa aagtaggttt tgtttcactc cacagatgtc tatatttggt
     4441 taaactatca attccctctc ccgttaaaag taaaacactg tttataccag catttttggc
     4501 caacatcata tcgtttttac cggaatcgcc tactacgaaa cacttacttg gatttaaatc
     4561 gtattttttt aatgcttcat tgacaaatgt aggttgaggc ttcttgcaat cacaattgtc
     4621 ttttctagca tgagggcaag tgtatacatc cgttatttga atgtcatttt tctctaactc
     4681 atttattaag ctctctaagg catcagtata ttctgccata gtaatacgtc catgtgaaat
     4741 atgactttga ttcgtaatga tgatatttag ataatgactt tgtgccgctt tttttaaggc
     4801 cggtattgtg tctttaaaaa aattgaaatt ttgattaatc agtgttctac tatcccctcc
     4861 cagcgtacct aacagatccc aaaatatagc tttattcatg gatatttgtc tccctctcat
     4921 tttaagaata gttaactaac aagccaaggt gcttacagta tttaaaagta gttcttcgta
     4981 attaaaacaa aaatattact ttttgaaaaa gcatatttta ttttgccagt ttaactatat
     5041 catatttaca ggagccactc tctattcact ttctatatga acttcttttg agggaatgga
     5101 tatttcgaat cacataatca ctgatataga agagacttag aatttcagtt atattttgat
     5161 acagcaaaga gtgtaaaaaa gaagataccc tcgttggcga aaaacatttg aatacaaacc
     5221 ttactttttt gatgggattt ccatgctagg agcacataaa aaactaaggt gataataaat
     5281 ttatagtgat ttagtgtgga ggttcttttg ttaattgaaa ccataaatag taaaaaaatc
     5341 tatttttaat gattgtacat attaaagaga aaattgatat acaccagctt atcaaagttg
     5401 agattaatac tactactttg acctatgaag atgattttat aaatacctta tagtaaagaa
     5461 aagttcaatt ttttctgcta tcatcttttt ttataaaagc aacgattagt ttcccagatg
     5521 taaatagaaa agtgcttttg aaacagctcg aaaaacataa taatagattg tgagaggagg
     5581 aaaaactatt gcaaaatcat tacgtaagag atgttactct tgcttagatt ttttattttt
     5641 caagttgtgg tattaaaagt agaaaaaata gaaagaagga taaaaaatga aaaataaatt
     5701 taagtattta tttactattt tagccttatt gccaattact acatcgacct taattactcc
     5761 tacaatagcc aaagcttctg aaaaacagga agattctgtt agttatgaaa ttgtaaaaaa
     5821 tgatctttac attcagtttg aagaagagtt agctgaattg attgcttcca atcaatcaac
     5881 tgtttcagtc cctgatttat tttctacaca atcagtatta tctacttcta gtcaagataa
     5941 ttctattagt gaagagcaat ttttttcttt agtcgaaaaa tatgatggag ctgaattgac
     6001 agctgttgtt aattcacaag atgctttcac aactaaattt gtgaatggtg gggttaactc
     6061 atatgctact tcttacagag gattaaatgc attgcgtagc gagttgaagt ataatgctgg
     6121 tgctttgaaa gtaggaggta ccttagttgg cacagctggg ggattgttag gagtattatt
     6181 aggcatggtt acaggggatt atttatctgg ccattgcgat gatgcagcag caggtgttag
     6241 tgcctttatt caagctggtt gaatacggca tcgaaaatgt accactagag cattgttttt
     6301 taccactaag tgcaaagaaa tgtaccagta agtgcgacgc ggttaccatt tcaaataata
     6361 aaagcgacaa taatgaccac aaggtcaact aagagccact catgaaaatc atggagtggc
     6421 tcttaattta ttttaatgac tcatttaatt acggatgccg taacgttcac gcatagaaac
     6481 tttaccgtct accagaattt gataagaatc atgtatgatc ctatctagaa tggcatcagc
     6541 gatttggggg ttgccgagct tttcgtacca tccttctgga tggaattgag aacagaaaat
     6601 agttgatgct gttttaagac gggattcgat tatttccaat agaaaggtgg aattttctaa
     6661 cgaaagattg gttaatagcc attcatcgat gatgagtaat tgaacttttt tatatctgtt
     6721 gattattttt cgaaaactac catctgcttg atttttcgag atggttaatt catctagtag
     6781 ttcagggagt cggatgtatt tcacgttgta gtattgccgg catgcctgta ttccgaaggc
     6841 attggcgata tacgttttcc cattccctga agcgcccatc aaaatgatgt tatggtgttc
     6901 ttccaaataa gtcccactgg ctagttttaa aatgaggttc ttatctaaat tacggtccgg
     6961 atggtattcg atatcctcaa tgctggcaga ggcatttcta aagtttgcag atttaatcaa
     7021 ccgctgtagt ttattatttt ttcgccggga atgctccaga tcaaccatga ggttgaagcg
     7081 gtcttcgaaa ctcatggatt ggtactcttt atttgtcatc tgttcacgat aggattcaag
     7141 catgcctgaa agtttcaatt cagctaattt tacttctgtt tgctcattta acatcattat
     7201 ttccctccaa aataggacgc accgcgagtg aatccatgct tttcaatgtt agtgtcttca
     7261 atatgtgatt ggcttgttgc tcgcttctgc ttgttgccct ttagaatggt ctggataatt
     7321 ttaacagaag gcaaagaagc cagatttaaa gctgttgaac aggccgtttc tatttcattt
     7381 ttagaatact ttttaccaaa gctcttcaaa gacatgatcg actttaaggc ttgtttttca
     7441 ttttgataac ggtctaagag gtgttgaaca acctttactg ttgaggaacc gatggtttca
     7501 gcccattcca tcgaggattc tggggtatgg tccacataca ttttgtgatt atctggcata
     7561 tgatctctga cagtcgaata ctgcccaaat ttcccgtaca gacgtttatg tgttgcgatt
     7621 cgcatggaat taaaatatag ttcaattaga ttctctgtca ttcgaatatc tatattttgt
     7681 tgaatgtatt cgtatggaac ggaataaaac atgctttcaa ctgagacgtg gtagtttagt
     7741 tgaacctttg cttttttcca gacagacatc ttataaggtg ccagtggaag aggattaagt
     7801 gcgaattttt cttcttcctc gaaggctctg atcctggatc ctggtttctt agtgaacggt
     7861 cgctcattga attcatttaa tttcattctg atttcacgat ttaaatcttc gattgtaaaa
     7921 caatgcgtat tgcgtaaggc agctataatc caagtggaga tgacgcctac gctgccttca
     7981 acactgggct tatccttggg agtagaaact cgggcaggta aaatgatgga cccgtaatgt
     8041 tcccctagct ctcgatatga ttcattaagg atgacatcga tgccttcatt cttttttaca
     8101 cctgttttta gattatcggg aacaattatg tcggtagaac cgccaaaata ttcaaatgca
     8161 tggttatgtg ccgtaatcca agccaccgat ttcatcgtta ggaatgcttc cgcataagac
     8221 aattggctgc acggtaaggt tgccacaaag acataagcct ttactttttc acccgtatct
     8281 cgatcgatga cataagctgg cgatccagcc caatccactt ccataatttc accaggcttc
     8341 cgacggatgc gcatagtggc tttatgcttt cgcgcgaacg cattgtaata gtcacaaaat
     8401 gtacgatagg cgtaagggat tttcccactc atgcgacact caactgcgta ttcctgatga
     8461 agaagcgtca aggtcacgtt tggtttggcc aattccttgt gaatcttgtc gaagtcaggc
     8521 aggtgccgtc cattggcttc taattgtttt tctggaaaca aaaattcgcc tagccattga
     8581 tcggtcattt cttccgacaa cgccctcgct atccctttct cctcagcttt tcggataacc
     8641 tcagtaattt tctgacgtga gttccccgtg cttgctgcaa tactgcgaat actaattcct
     8701 tcactgtgca tttcaagtat ccttcgataa cgaaacataa taaaaatacc tcctgtaaaa
     8761 taaatgtcac accctaaggt gtgcacctac attatacaag aggtatttgt ggtacactgc
     8821 tttgcactta ctggtacatt tctttgccga gcctggtaaa aattattgca gagatggtac
     8881 attttcgatg ccgtaatcac tggttacact agaggaggat ctagggtaac ccttactgaa
     8941 ggtttcccta ttagccatat tgatagtatt tatgaagctg taattaatta agcaagaaat
     9001 gtggagatga taaaaagcag ttctctcttg ggtaaagaga aaactgcttt tatctttttc
     9061 gctaatgctt tttggaatta cctcgctgtc taaaaaggga agtcacagaa aaaagaatta
     9121 acacactacc tattattatt ggtagtacag ttaaaagagt aatcttacta agatcaaaat
     9181 cagaacttgc tacactatta ataacaaaaa acaagccaac caaaaaagta aatacagata
     9241 gatatttatt agtcataaaa gaatcttcct ctcgtatttt tttgttctag gaagctatgc
     9301 taatctaagt tcttcctaag tatagtgtct catctaagta tagcttaatt aagcatctta
     9361 tggtttacat caggtcactt tatagttgca accgctcaaa tttgcttttt gattttatta
     9421 tctacgttat tagtaaaaaa ttagttgatt tggttttttt atttcgttga tattttatct
     9481 gtttagtaag tagagttggt aacagtgaat gtttactaac ccagatacac ttatatatgc
     9541 gtccatttgt agagagagcc gtttcaatat aagaatgttc acaaaatgtt ctcttactaa
     9601 ttaaacccgt atatattaaa ctatgagtat attaatttta ggagggattt tttgagacaa
     9661 gttttattgg ttgttacttt atgtttatct ttctttttat ttgaagtaaa cactgtagaa
     9721 gcatatgtgg caaataatgg agtctactat aacttaatcc aaggtggcac gcaagagttc
     9781 atggaggtag acgaagaagg aaatcctatc ttgattcgtg tggaagaggt tcccgggctc
     9841 tccctgttct ccgtcaacaa cggaaattat aaaatatctg gatctaaatt tggaatgtgg
     9901 aaggcaaatt actatgtgac agtctcgaat aaccaattta ttagggccta ctccccttct
     9961 gcagtcgcag tgaccggatc ctttactagc acgagactgt ctctcgtctc cagcaaaaaa
    10021 gctagctaca cattagaatg gaaagctgga attttatcta gtaactctta tctagatgct
    10081 acactgacag ataattccat taatattacg tattagttct caatatattc cacgttagtg
    10141 gagccgcccc aatgaaagat attactaaaa tgtccatagg agacaaacag gcttactgta
    10201 aaacagatga ctgaagcagc aatcactcct ttataaaaag tgtttgttgc tttatttctt
    10261 ttcagaacaa caggcgcaat aattagtcca agaggagaaa tcaaacagcc gattaaggaa
    10321 atgagcaata aaactaaccc ttcatctcgg tttgtattat tttttaaata aattaattta
    10381 tttttagatt ctattatctc tatactattc tcttttatat tttttttaac ttcttttttt
    10441 tcttttaaaa gatcatcgat agagatttca tataaactac ttaataaagt taagttgttt
    10501 atatctgggg aactagtatc attttcccat ttagaaatag attgtctaga tatatttaaa
    10561 tattttgcta cttcactttg agagatgcct tttatatgcc gtgatttttt aatttttccc
    10621 ctaatgtcat tctgtatcct ctcctattaa tgacttttat ttattggata ttaagaaata
    10681 gattatttag tctatattca agtgataatc tagttgtttt tgaaggatat tctgaacttc
    10741 ttctaaagat tcttcgttaa tcatttcata gtatattccg tcatcaagta gttgtccttc
    10801 cccctttaat tgaacttgct ctatatttcc taaagcagtg gaatatttat taataatagt
    10861 ccacatatcg tcaactgaca gattagtagt catatttccc tgaagactct taatgagtga
    10921 aggtatctta gttgctagtg tagtaacttt agttcctttc tctataattg ccattattat
    10981 ttccctttga cgagcttgtc tcccatagtc acctgaatca tctgcatgac gatttcgaga
    11041 gtacgctaag gaagccttcc catctaagtt aataatttcg ccttgataaa atgaataact
    11101 atcttgtgta aaagtggcag ttggagtaag agttattcct ccaatagaat caacaatttg
    11161 ttctagaccc tgcatattta acgttacaaa aaagtcaata ggtatatcta agtatgcttg
    11221 tattgtattt atagtcatgg ctactccacc aaaggcatag gcatggttaa ttttgtcgta
    11281 agtatcataa ccgataatat cgacataacg gttctgttgc aaagttttaa atctactatc
    11341 aaataag
//
LOCUS       DACGLE010000216         8061 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0534
            SAMEA3304449-rid9560453.guided.104, whole genome shotgun sequence.
ACCESSION   DACGLE010000216 DACGLE010000000
VERSION     DACGLE010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304449
            Sequence Read Archive: ERR985064
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8061)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8061)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702225.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702225.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 20:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,045
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 27 of 67
            Pseudo Genes (incomplete)         :: 25 of 67
            Pseudo Genes (internal stop)      :: 19 of 67
            Pseudo Genes (multiple problems)  :: 4 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8061
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304449-rid9560453.guided.104"
                     /strain="VREN0534"
                     /db_xref="taxon:1352"
     gene            128..1564
                     /locus_tag="IXT20_003020"
     CDS             128..1564
                     /locus_tag="IXT20_003020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAQ4861378.1"
                     /translation="MEHKELTSFPKDFLWGSASAAYQVEGAWQEDGKGESVWDRFVRI
                     PGKTFKGTNGDLAVDHYHRYKEDIALMKEQGLKAYRFSVAWTRIFPNGRGEVNQAGLA
                     FYERLIDELIENDIEPMLTLYHWDLPQALQDEYNGWESRQIIEDFTHYAETLFEAFRG
                     KVHYWISLNEQNIFTSLGYLLAAHPPGVTDPKRMYEVNHIANLANASVINKFHELEMP
                     GKIGPSFAYTPNYPIDSNPENVLAAENAEDLMANYWMDVYMWGKYPIAAMRFLEEKGW
                     APTIEAGDAELLESAKPDFLGINYYQTATNAFNPLNGVGAGKMNTTGKKGSSEETGVP
                     GMYKKVENPFVERTNWDWEIDPEGLRIGLRRITSRYRMPVLITENGLGEYDKLTEDKQ
                     IHDDYRINYLQSHVKAIKEAISDGAEVLGYCTWSYTDLLSWLNGYQKRYGFVYVDQDE
                     TQEGSLERYKKDSYYWYQKLIEENGENL"
     gene            1753..4200
                     /locus_tag="IXT20_003021"
     CDS             1753..4200
                     /locus_tag="IXT20_003021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HAQ4861379.1"
                     /translation="MAQKKRPTKKRKKTKKTKKQKQQQEQMLTIGVGFALILFAVFGF
                     LKLGFLGILIANGFRIIAGNTYQILCLLLAVLGFWIVIKNTEFSIGKNRRWFGGILFY
                     FGILLLLHAHLFGKLHTGEPNIMGTTWDLLASDIKQSQVDNNVGGGMIGAILYHFTYF
                     LIAQPGSYLAAVLLLAGGAFLFSNLEGYQLLNGIQSIGERVQELLEGDPAKQARKQAA
                     KEERMKQRAEAKEARRLAAKEAAEKEAAEYEKKKAVSQQRENTADEWQEPENTEPEQL
                     SFVPIDSFQENIHPANLEKPVPDTPKQTNTAEGFSDELPEDDGTSLEFEIEAEQENQD
                     YELPTVDLLDSIPTVDQSDEYKKIEKNIGVLEQTFQSFGVDAKVVKASLGPSVTKFEV
                     QPAVGVKVSKIVNLTDDIALALAAKDVRMEAPIPGKSLIGIEVPNGKISMVSFREIIE
                     AQPNHPDKLLEVPLGRDVSGRVQTADLSKMPHLLVAGSTGSGKSVAINGIITSILMRA
                     KPHEVKLMMIDPKMVELNMYNGIPHLLTPVVTNPRKAAQALQKVVQEMEERYEKFAAT
                     GVRNISGYNEFVQQKNLENGTKHPTLPFIVVIVDELADLMMVASNEVEDAIIRLAQMA
                     RAAGIHMILATQRPSVDVITGIIKANVPSRMAFAVSSGTDSRTIIDSNGAEKLLGRGD
                     MLFLPMGENKPIRVQGAFISDHEVERVVQFVTDQQEAHYEEKMMPTDEVETTGAPEQP
                     QDELFEEAKALVVEMQTASISLLQRRFRIGYNRAARLVDELEAHGVVGPSEGSKPRKV
                     FIEQEETSETLPDDNDM"
     gene            4430..5497
                     /gene="rlmN"
                     /locus_tag="IXT20_003022"
     CDS             4430..5497
                     /gene="rlmN"
                     /locus_tag="IXT20_003022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HAQ4861380.1"
                     /translation="MEKQSIYGLTNEELINWFIENGEKKFRAAQVWEWLYQKRVSNFT
                     EMTNLSKQLIEKLSEHFIINPLKQMVVQEASDGTVKYLFELPDKNMIETVLMRQEYGL
                     SVCVTTQVGCNIGCTFCASGLLKKNRDLTAGEIVAQIMMVQHYFDERNLGERVSHVVV
                     MGIGEPFDNYDNVMDFLHIINDAKGLAIGARHITVSTSGLAHKIKEFANNGLQVNLAI
                     SLHAPNNEVRTSIMRINRSFPIEKLMEAVDEYLEKTNRRITFEYIMLNQVNDRPEHAQ
                     QLADLLKDKKKLAYVNLIPYNPVSEHDQYSRSPKADVLRFYDVLKKNGVNCVIRKEHG
                     TDIDAACGQLRSKQMKKTVAK"
     gene            complement(5690..6793)
                     /locus_tag="IXT20_003023"
     CDS             complement(5690..6793)
                     /locus_tag="IXT20_003023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HAQ4861381.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
     gene            7346..7894
                     /gene="aac(6')"
                     /locus_tag="IXT20_003024"
     CDS             7346..7894
                     /gene="aac(6')"
                     /locus_tag="IXT20_003024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HAQ4861382.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT         2695 a         1374 c         1751 g         2241 t
ORIGIN      
        1 tttcttacta ctcgaaacta agcgactttt tcacaacctc ctttacatat ttttattttg
       61 ttgtggaagg ggtttcgtgt tattctaaaa gaggaacgat tgaaggttat aggaaaggaa
      121 gagatgaatg gaacacaaag aacttacctc atttccaaaa gattttctgt ggggctctgc
      181 ttctgcagcg tatcaagtag aaggagcttg gcaagaagat gggaaaggag aatcagtctg
      241 ggatcgattt gtacggatac ctggaaagac atttaaagga acgaacggtg atttggcagt
      301 agaccactat catcgttata aagaagatat tgctttaatg aaagaacaag gtctgaaagc
      361 ttaccgcttc tctgtagctt ggacacggat ttttcctaat ggacgaggag aagtcaatca
      421 agctgggctc gctttttatg agagattgat cgacgaattg attgaaaacg atattgaacc
      481 tatgttgact ttatatcatt gggatcttcc acaagcattg caagatgaat ataatggttg
      541 ggaatcacgt cagatcattg aagatttcac tcattatgca gaaacattat ttgaggcatt
      601 ccgcgggaaa gtccattact ggattagttt gaatgaacaa aatatcttca cttcattagg
      661 ttatttgtta gcagcacatc cgccaggtgt gacagacccg aaacgaatgt atgaagttaa
      721 ccatattgct aatttagcaa atgcatctgt catcaataaa tttcacgagt tagaaatgcc
      781 tggaaaaatc ggaccaagct ttgcgtatac gccaaattat ccaattgaca gtaatcctga
      841 aaatgttctg gctgctgaaa atgcagaaga cctgatggcc aattattgga tggatgtcta
      901 tatgtgggga aaatatccga ttgctgccat gaggttctta gaagaaaaag gatgggcacc
      961 aacgattgaa gctggcgatg cagaattgct agaatctgct aaaccagatt ttcttgggat
     1021 caattactat caaacggcga cgaatgcatt caatccatta aacggagttg gtgctggaaa
     1081 aatgaatacg acaggaaaaa aaggttcatc agaagaaact ggagttcctg gaatgtataa
     1141 aaaagttgag aatccatttg tcgaacgaac aaattgggac tgggagattg acccagaagg
     1201 attaaggatc ggactgcgtc gtatcacaag ccgttaccgc atgccggtac tgattacgga
     1261 aaatggctta ggagagtatg acaagttgac agaagataaa caaatccatg acgactatcg
     1321 aatcaattac ttacaaagcc acgtcaaagc catcaaagaa gcaatttcgg atggagcaga
     1381 agtcctaggc tattgcacct ggtcttatac agatttgctt agctggctga acggttatca
     1441 aaagcgatat ggatttgtat atgttgatca agacgaaaca caagaaggat ctttggaacg
     1501 atataagaaa gacagttatt actggtacca aaaactgatt gaagaaaatg gagagaacct
     1561 ttagttcaca tacttaaaac aagaaggtac ggctggatat ggccgtgcct tttcttgata
     1621 aaatcattta gaaattaaga aaaactgtaa aataagctga aaaatgaaag aaagcgtcag
     1681 ttctttcaaa caaaacttta gttcccttac ttttttttgt ataatgaaac ttgatgaaat
     1741 gagggataat aaatggcaca aaagaaacgg cctactaaaa aaaggaaaaa gacgaaaaaa
     1801 acgaaaaagc aaaaacagca gcaggaacaa atgctgacga ttggtgttgg gtttgccttg
     1861 attttatttg ctgtcttcgg ctttttaaaa ttaggttttt taggaatatt gattgcaaac
     1921 ggctttcgga tcattgcagg aaatacttac caaatcctct gtcttttatt agccgtatta
     1981 ggattttgga ttgtcattaa gaatacagag ttttcaattg gtaaaaaccg acgctggttt
     2041 ggcggcatac tcttttattt cggtatctta ttattgttac atgcccattt atttggcaaa
     2101 ctccatactg gggaaccaaa tatcatgggg acgacatggg atttgttagc cagtgatata
     2161 aaacaaagtc aagtagacaa taacgttggt ggtgggatga ttggagcaat cctttatcat
     2221 tttacttact tcttgattgc acagcctgga agttatttag cagcagtgct attactggca
     2281 ggaggagcat tcctgttcag taatctggaa ggctatcagc tgttaaatgg tatccaatca
     2341 attggagaac gagtacagga attattggaa ggtgatcctg ccaagcaagc tcgaaagcaa
     2401 gcggcaaagg aagagagaat gaaacagcgc gcagaagcga aagaagcaag gcgcttagct
     2461 gcaaaagaag ctgcagaaaa agaagctgca gaatatgaga agaaaaaagc ggtgagccag
     2521 caaagggaaa atactgctga tgaatggcag gaaccagaaa atacagagcc ggagcagcta
     2581 agttttgttc caatcgacag ttttcaagaa aatatccatc ctgcgaatct agagaaacca
     2641 gtaccagata caccaaaaca gaccaataca gcagaaggtt tttcagatga gttgccagag
     2701 gatgacggta cctcattaga atttgagatt gaagcagaac aggaaaatca agactatgaa
     2761 ttaccgacag ttgatttgct tgattctata ccaactgtgg atcaaagtga tgagtataaa
     2821 aaaattgaaa aaaatatcgg cgtattagaa caaactttcc aaagtttcgg tgtcgatgcc
     2881 aaggtcgtca aagccagctt aggaccatcc gtcacaaaat tcgaagtcca gccggctgta
     2941 ggggtaaaag taagtaaaat tgtgaactta acagatgata tcgcgcttgc tttagcggcg
     3001 aaagatgtcc ggatggaagc accgattcca ggtaaatcac tgattggtat tgaagtacca
     3061 aatggaaaga tcagtatggt ttcctttaga gaaatcatcg aagcacaacc aaaccaccca
     3121 gataaattac tagaagtacc tttaggacgt gatgtttctg gacgggtaca aacggctgat
     3181 ctttcgaaga tgcctcactt actcgtggct ggttcgactg gtagtgggaa gtcagtagca
     3241 attaatggga ttatcacaag tatattaatg cgtgctaagc cccatgaagt aaaactgatg
     3301 atgatcgatc cgaaaatggt tgaattgaat atgtacaacg gcatacctca tctgttgacg
     3361 ccggtcgtga cgaaccctag aaaagcagcg caagccttac aaaaagtagt acaagaaatg
     3421 gaagaacgat atgaaaaatt cgcggcaacc ggagtaagaa atatttctgg ttacaatgaa
     3481 tttgttcaac agaaaaactt ggaaaacgga acgaaacatc caaccttacc attcatcgta
     3541 gtgatcgtag atgaattagc tgatttgatg atggtagcca gcaatgaagt tgaagatgcg
     3601 attattcgtt tagcgcaaat ggctcgggca gcggggattc atatgatcct agctactcaa
     3661 cgtccaagtg tggatgttat cacaggaatc atcaaagcaa atgtaccttc cagaatggca
     3721 tttgcggtat caagcggtac agattctcgg acgatcattg attctaatgg ggcagaaaag
     3781 ctcttagggc gaggagatat gctattcttg ccaatgggtg agaataaacc gatccgtgtg
     3841 caaggagcat tcatttctga ccacgaagta gaacgggtcg ttcaatttgt tacagatcag
     3901 caagaagctc attatgaaga gaaaatgatg ccgacagatg aagttgaaac gacaggtgct
     3961 ccagaacagc cacaagatga gctgtttgaa gaagcaaaag cacttgtcgt tgaaatgcaa
     4021 acggcaagta tttcccttct ccaaagaaga tttcggatcg gttacaaccg agcagcacgt
     4081 ttagtagatg aactggaagc tcatggagtt gttggcccgt cagaaggaag caagcctcgg
     4141 aaagtattca ttgagcagga agaaacatca gaaacgttgc cggacgataa tgacatgtaa
     4201 aacttgtgtg atgaagggtt gaaaaacgaa taaccaataa aaagacaagg catgatgata
     4261 ccaaaagaaa aaatggtttt tttccatctt ttctaatacg gtatgatgtc ttgtcttttt
     4321 agtttttgtg agattaaagt tctattcgat ttatttgtga agtattgcat taaggaaatg
     4381 aatcatgtat agtatgtagc gaaactttga gaaaaagagg gaaaatactt tggaaaaaca
     4441 gtccatttat ggcttgacga atgaagaatt aatcaattgg tttatagaaa acggcgagaa
     4501 aaaattccga gctgcccaag tttgggaatg gttgtatcaa aaacgtgtaa gtaatttcac
     4561 agaaatgacg aatctatcga aacaattgat cgaaaaatta tcggagcatt ttatcatcaa
     4621 tcctctaaaa cagatggtcg tccaagaagc ttcggatggt acagtaaaat atttgtttga
     4681 actccctgat aaaaacatga ttgagactgt actgatgaga caagaatatg gcttgtcagt
     4741 ttgtgtgacg acacaagtag gatgtaatat cggctgtacg ttttgtgcaa gcgggctgct
     4801 caaaaagaac cgtgatctga ctgctggaga aattgttgca cagatcatga tggtccaaca
     4861 ttattttgat gagcgcaatc ttggcgaacg cgtttcacat gtcgttgtaa tggggatcgg
     4921 tgagccattt gataattacg acaatgtaat ggactttttg catatcatca atgatgccaa
     4981 aggattggca attggtgctc ggcacattac agtttctact agtggattag cacacaaaat
     5041 caaggaattc gctaataacg gcttgcaagt caatctggct atttccttgc acgcgccgaa
     5101 taatgaggtg cgaacatcga tcatgcggat caaccgaagt ttcccaattg agaaattgat
     5161 ggaagctgtg gatgagtatt tagagaaaac aaaccgtcga atcacgtttg aatatatcat
     5221 gttgaatcaa gtaaatgata gaccagagca tgcgcaacaa ttagcagatc tattaaaaga
     5281 taagaaaaaa ttagcatatg tcaacttgat tccatataat ccagtcagtg aacatgatca
     5341 atattctcgc agtccaaaag ctgatgtatt gagattttat gatgtattga agaaaaacgg
     5401 cgtaaactgt gtgatacgta aagaacatgg tacagatatt gatgcagctt gcggacaatt
     5461 gcgaagcaag caaatgaaaa aaacagttgc caaataatcg tatcctactc gttttttcaa
     5521 ttaccataaa aggagcagtt ttcttaatta aggaactgtt caacttaaat aagagaaaag
     5581 attctatagc gaaagctata aagctttttt tgacaagaaa aaccctcatc aagcaaagaa
     5641 atgtcctcaa tttgatgaca gcctgcttga tgggggtttt agcaattatt taactgattt
     5701 tcttatatcc ttgctggctt tcttgtgtag catgataagt ttctacgtca agaatggcct
     5761 gtttaaattt ttctcgggtg atttcataag gcacatgttt aatatcattt gttgccatgg
     5821 ctttatctaa gattgcttct aaatcggtcg tgacattgag attcatgtcg gttaacgata
     5881 ctggaagttg gttctttttc ataaacacat agattttatc aaacaaatcg aactgctgat
     5941 ccatcatcaa taaggtcagt acaccataac tgacagatgc tccgtgaagg tatccctcca
     6001 ttttttctag aacagtcgta ccgtaataga atgagtgtgc catagcagaa ttatagttat
     6061 tgttaactaa aacagaaacc aatccggtag taacaatgat atccgaaacg acttgttcaa
     6121 ttgcatatga gggacgatcg tcggcactat ctttcaatgc ttgcgtacca taagtcaaga
     6181 gacgatcaat gcacattttt ccgatttgga ttcctaatct atccgtgtag cctagatcat
     6241 cgccacgagc agaaaaagta gattcgactt ctttggataa agcatcgcca atacctgccc
     6301 ataagtattt atcaggtgct tcggcaataa ttgctgtatc aatgaaggtg tgtacaggtg
     6361 gttttgaagg aaagaagagt ccgtccatta cgccttcttc gttatagatg acacataccg
     6421 cagtgacggg cgaacagttc gaaccaatag tgggaaaggt gaagactggt ttagctagtt
     6481 tttcaccaat gacttttaca gtatcgcaag cacggccacc accgactgca aacaggacgt
     6541 ctgctttttg gacactttca tgctggatca actgttcaat atttccataa gttgaattac
     6601 ccccgtacca gagactggcg acaacctcta tttcctgtgt caaagctact tttattttgt
     6661 tttctgcctt tgaaagtgca gtcttccctc caatcagcac tacttttgtc ccatattctt
     6721 tgacgattcc atttgctttt tcatacgcat ctgttccaat ggtataattt ggtaagaaaa
     6781 cactttgact catattcatt tccttctttc ttcgttgaaa cgggttaaaa ttttaaagag
     6841 tgatcgatct gactgcggct gtagatggag tagaggtgaa cctaagacag gataagtgat
     6901 gaataaggtg gcatgaacac cttgaaatca tcaataagta tcctaaaaac gaacaagtac
     6961 aaaaacattg aaaacgacaa taatgaattt ttgttcctct tagtatttct accgattccc
     7021 tttgcttttt tgtaattatc taaaaattct aaaaattaag attattatat gaaaacaggt
     7081 tttatttgtc aattcataat tggttttttt taaagcaaaa gataatctat atcatgatga
     7141 acaaacagag ttttttcctc caattgatgg ctttcctgat ctttcctatt ttgaataaag
     7201 tgtttctgta taattttttt attttataag taagataaat aatgaaagaa tagagatact
     7261 gattggtagc taaagatttt tgagcgagaa catttcgttt tttcaaaagg actgttagaa
     7321 taagcaagaa caagggggaa gacgtatgat aatcagtgaa tttgaccgta ataatccagt
     7381 attgaaagat cagctttctg atttactgag actgacttgg ccggaagaat atggagacag
     7441 ctcggcagaa gaagtagaag aaatgatgaa tccagaacga atcgcggtag cagcggtaga
     7501 ccaagatgag ttagtaggat ttattggtgc aatccctcaa tacggtatca caggttggga
     7561 attgcatcca ttagttgtag aaagctcccg acgaaagaac caaataggta ctcgattagt
     7621 caattactta gaaaaagaag tagcttccag aggaggaatc acgatttatt taggtacgga
     7681 tgatttagac catggaacaa cgttaagtca aacggacctg tatgagcata catttgataa
     7741 agtggcttcc atccagaacc ttcgtgaaca tccgtatgaa ttctatgaaa aattaggtta
     7801 taaaatcgta ggtgtcttac caaatgcaaa tggctgggac aaaccggata tttggatggc
     7861 aaaaacgatt attcctcgac caaattctca ataataaaaa gaggttgtga cagaagtaat
     7921 cagcttcaag aaataagaag gaatttacga aaattacttc ttgaattttt gtgaaattcc
     7981 aacttatttc cgaagaagct acttttggaa caccgtgtac cagaggttgt gaaaaagtcg
     8041 cttagtttcg agtagtaaga a
//
LOCUS       DACGLE010000217         5093 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0534
            SAMEA3304449-rid9560453.guided.125, whole genome shotgun sequence.
ACCESSION   DACGLE010000217 DACGLE010000000
VERSION     DACGLE010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304449
            Sequence Read Archive: ERR985064
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5093)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5093)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702225.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702225.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 20:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,045
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 27 of 67
            Pseudo Genes (incomplete)         :: 25 of 67
            Pseudo Genes (internal stop)      :: 19 of 67
            Pseudo Genes (multiple problems)  :: 4 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5093
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304449-rid9560453.guided.125"
                     /strain="VREN0534"
                     /db_xref="taxon:1352"
     gene            complement(37..2328)
                     /locus_tag="IXT20_003025"
                     /pseudo
     CDS             complement(37..2328)
                     /locus_tag="IXT20_003025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000684984.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
     gene            2474..3049
                     /locus_tag="IXT20_003026"
     CDS             2474..3049
                     /locus_tag="IXT20_003026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016204422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAQ4861383.1"
                     /translation="MRKIGYIRVSSTNQNPSRQFQQLNEIGMDIIYEEKVSGATKDRE
                     QLQKVLDDLQEDDIIYVTDLTRITRSTQDLFELIDNIRDKKASLKSLKDTWLDLSEDN
                     PYSQFLITVMAGVNQLERDLIRMRQREGIELAKKEGKFKGRLKKYHKNHAGMNYAVKL
                     YKEGNMTVNQICEITNVSRASLYRKLSEVNN"
     gene            3263..3958
                     /gene="vanR"
                     /locus_tag="IXT20_003027"
     CDS             3263..3958
                     /gene="vanR"
                     /locus_tag="IXT20_003027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance DNA-binding
                     response regulator VanR"
                     /protein_id="HAQ4861384.1"
                     /translation="MSDKILIVDDEHEIADLVELYLKNENYTVFKYYTAKEALECIDK
                     SEIDLAILDIMLPGTSGLTICQKIRDKHTYPIIMLTGKDTEVDKITGLTIGADDYITK
                     PFRPLELIARVKAQLRRYKKFSGVKEQNENVIVHSGLVINVNTHECYLNEKQLSLTPT
                     EFSILRILCENKGNVVSSELLFHEIWGDEYFSKSNNTITVHIRHLREKMNDTIDNPKY
                     IKTVWGVGYKIEK"
     gene            3936..5087
                     /locus_tag="IXT20_003028"
     CDS             3936..5087
                     /locus_tag="IXT20_003028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000791889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAQ4861385.1"
                     /translation="MVIKLKNKKNDYSKLERKLYMYIVAIVVVAIVFVLYIRSMIRGK
                     LGDWILSILENKYDLNHLDAMKLYQYSIRNNIDIFIYVAIVISILILCRVMLSKFAKY
                     FDEINTGIDVLIQNEDKQIELSAEMDVMEQKLNTLKRTLEKREQDAKLAEQRKNDVVM
                     YLAHDIKTPLTSIIGYLSLLDEAPDMPVDQKAKYVHITLDKAYRLEQLIDEFFEITRY
                     NLQTITLTKTHIDLYYMLVQMTDEFYPQLSAHGKQAVIHAPEDLTVSGDPDKLARVFN
                     NILKNAAAYSEDNSIIDITAGLSGDVVSIEFKNTGSIPKDKLAAIFEKFYRLDNARSS
                     DTGGAGLGLAIAKEIIVQHGGQIYAESNDNYTTFRVELPGFCCKVLNKE"
BASE COUNT         1598 a          957 c          982 g         1556 t
ORIGIN      
        1 gggattttat tctttattta aaactttgca acagaaccgg tcagtgtaac cggcagtgtc
       61 tgtataatgt tcctctatgt ttagatccgt ctcatgatgt aacaaaccat ccaaaacatg
      121 aatcgcatct cttgaattag tatgaataat ctttgtgtag taagaagaga attgatcact
      181 tgtaaatcgg tagatggtgg ctccttttcc agttccataa tgtggatttg catctgcatg
      241 tagtgatgaa acacctagct gcattctcat accatctgac gaagatgttg taccgtcgcc
      301 ccaatagaaa ggcaattgta atttatgatg aaagtttact aatatggctt gggctttatt
      361 catggcatct tcatacatgc gccattgaga tacattggct agttgcttat atgtaagtcc
      421 gggtgtggct tcggccatct tgctcaagcc aatattcatt cccattccta aaagggcagc
      481 catgataatg attgtttctt ccttatctgg ttttcgatta ttggaagcat gagtgaattg
      541 ctcatgaaat cctgttatat gggccacatc catgagtaaa tcagttaatt ttattcttgg
      601 tagcatctga taaaggcttg cactaaattt ttttgcttct tctggaacat ctttttctaa
      661 gcgtgcaagt gatagctttc ctttttcaag agaaacccca tctaacttat tggaattggc
      721 agctaaccac tttaaccttt cattaaagct gctggttctc tccgttatat aatcttcgaa
      781 tgataaacta actgataatc tcgtattccc cttcgattga ttccatgtat cttccgaaaa
      841 caaatattcc tcaaaatccc tatattgtct gctgccaaca atggaaacat ctcctgcccg
      901 aacatgctcc cgaagttctg ttaaaacagc catttcatag taatgacgat taattgttgt
      961 accatcatcc tcgtataaat gtcttttcca tcgttttgaa ataaaatcca caggtgagtc
     1021 atcaggcact tttcgctttc cagattcgtt cattcctcgg ataatctcaa cagcttgtaa
     1081 aagtggctca tttgcctttg tagaatgaaa ttccaatact cttaatagcg ttggcgtata
     1141 ttttcttagt gaataaaacc gtttttgcag taagtctaaa taatcatagt cggcaggacg
     1201 tgcaagttcc tgagcctctt ctactgaaga gacaaaggta ttccattcaa taaccgattc
     1261 taaaacctta aaaacgtcta atttttcctc tcttgcttta attaatgctt gtccgatgtt
     1321 cgtaaagtgt ataactttct catttagctt tttaccgttt tgtttctgga tttcctcttg
     1381 agccttacga ccttttgata acaaactaag tatttgccta tcatgaattt caaacgcttt
     1441 atccgttagc tcctgagtaa gttgtaataa atagatggtt aatatcgaat aacgtttatt
     1501 ttcttgaaag tcacggaatg catacggctc gtatcttgag cctaagcgag acagctgcaa
     1561 caggcggtta cggtgcaaat gactaatttg cactgtttct aaatccattc ctcgtatgta
     1621 ttcgagtcgt tctattattt ttagaaaagt ttcgggtgaa ggatgacccg gtggctcttt
     1681 taaccaaccc aatatcgttt tattggattc ggatggatgc tgcgaggtaa taatcccttc
     1741 aagcttttct ttttgctcat ttgttagaga tttactaacc gtattaaata gcttcttttc
     1801 agccattgcc cttgcttccc acaccattct ttcaagtgta gtgatagcag gcagtataat
     1861 tttgtttttt cttagaaaat ctatgcattc atgcagtaga tgaatggcat caccattttc
     1921 caaagctaat tgatgaaggt acttaaatgt cattcgatat tcactcaggg taaaagttac
     1981 aaagtcgtat tcacttcgaa tttctttcaa atgatcccaa agtgtatttt ccctttgagg
     2041 ataatgatca agcgaggatg gactaacacc aatctgtttc gatatatatt gtatgaccga
     2101 atctgggatg cttttgatat gagtgtatgg ccaaccggga taccgaagaa cagctaattg
     2161 aacagcaaat cctaaacggt tttcttccct ccttcgctta ttaactattt ctaaatcccg
     2221 tttggaaaaa gtgaagtagg tccccagtat ccattcatct tcagggattt gcataaaagc
     2281 ctgtctctgt tccggtgtaa gcaattctct acctctcgca attttcattc agtatcattc
     2341 catttctgta ttttcaattt attagttcaa ttatatatca atagagtgta ctctattgat
     2401 acaaatgtag tagactgata aaatcatagt taagagcgtc tcataagact tgtctcaaaa
     2461 atgaggtgat attttgcgga aaatcggtta tattcgtgtc agttcgacta accagaatcc
     2521 ttcaagacaa tttcagcagt tgaacgagat cggaatggat attatatatg aagagaaagt
     2581 ttcaggagca acaaaggatc gcgagcaact tcaaaaagtg ttagacgatt tacaggaaga
     2641 tgacatcatt tatgttacag acttaactcg aatcactcgt agtacacaag atctatttga
     2701 attaatcgat aacatacgag ataaaaaggc aagtttaaaa tcactaaaag atacatggct
     2761 tgatttatca gaagataatc catacagcca attcttaatt actgtaatgg ctggtgttaa
     2821 ccaattagag cgagatctta ttcggatgag acaacgtgaa gggattgaat tggctaagaa
     2881 agaaggaaag tttaaaggtc gattaaagaa gtatcataaa aatcacgcag gaatgaatta
     2941 tgcggtaaag ctatataaag aaggaaatat gactgtaaat caaatttgtg aaattactaa
     3001 tgtatctagg gcttcattat acaggaaatt atcagaagtg aataattagc cattctgtat
     3061 tccgctaatg ggcaatattt ttaaagaaga aaaggaaact ataaaatatt aacagcctcc
     3121 tagcgatgcc gaaaagccct ttgataaaaa aagaatcatc atcttaagaa attcttagtc
     3181 atttattatg taaatgctta taaattcggc cctataatct gataaattat taagggcaaa
     3241 cttatgtgaa agggtgataa ctatgagcga taaaatactt attgtggatg atgaacatga
     3301 aattgccgat ttggttgaat tatacttaaa aaacgagaat tatacggttt tcaaatacta
     3361 taccgccaaa gaagcattgg aatgtataga caagtctgag attgaccttg ccatattgga
     3421 catcatgctt cccggcacaa gcggccttac tatctgtcaa aaaataaggg acaagcacac
     3481 ctatccgatt atcatgctga ccgggaaaga tacagaggta gataaaatta cagggttaac
     3541 aatcggcgcg gatgattata taacgaagcc ctttcgccca ctggagttaa ttgctcgggt
     3601 aaaggcccag ttgcgccgat acaaaaaatt cagtggagta aaggagcaga acgaaaatgt
     3661 tatcgtccac tccggccttg tcattaatgt taacacccat gagtgttatc tgaacgagaa
     3721 gcagttatcc cttactccca ccgagttttc aatactgcga atcctctgtg aaaacaaggg
     3781 gaatgtggtt agctccgagc tgctatttca tgagatatgg ggcgacgaat atttcagcaa
     3841 gagcaacaac accatcaccg tgcatatccg gcatttgcgc gaaaaaatga acgacaccat
     3901 tgataatccg aaatatataa aaacggtatg gggggttggt tataaaattg aaaaataaaa
     3961 aaaacgacta ttccaaacta gaacgaaaac tttacatgta tatcgttgca attgttgtgg
     4021 tagcaattgt attcgtgttg tatattcgtt caatgatccg agggaaactt ggggattgga
     4081 tcttaagtat tttggaaaac aaatatgact taaatcacct ggacgcgatg aaattatatc
     4141 aatattccat acggaacaat atagatatct ttatttatgt ggcgattgtc attagtattc
     4201 ttattctatg tcgcgtcatg ctttcaaaat tcgcaaaata ctttgacgag ataaataccg
     4261 gcattgatgt acttattcag aacgaagata aacaaattga gctttctgcg gaaatggatg
     4321 ttatggaaca aaagctcaac acattaaaac ggactctgga aaagcgagag caggatgcaa
     4381 agctggccga acaaagaaaa aatgacgttg ttatgtactt ggcgcacgat attaaaacgc
     4441 cccttacatc cattatcggt tatttgagcc tgcttgacga ggctccagac atgccggtag
     4501 atcaaaaggc aaagtatgtg catatcacgt tggacaaagc gtatcgactc gaacagctaa
     4561 tcgacgagtt ttttgagatt acacggtata acctacaaac gataacgcta acaaaaacgc
     4621 acatagacct atactatatg ctggtgcaga tgaccgatga attttatcct cagctttccg
     4681 cacatggaaa acaggcggtt attcacgccc ccgaggatct gaccgtgtcc ggcgaccctg
     4741 ataaactcgc gagagtcttt aacaacattt tgaaaaacgc cgctgcatac agtgaggata
     4801 acagcatcat tgacattacc gcgggcctct ccggggatgt ggtgtcaatc gaattcaaga
     4861 acactggaag catcccaaaa gataagctag ctgccatatt tgaaaagttc tataggctgg
     4921 acaatgctcg ttcttccgat acgggtggcg cgggacttgg attggcgatt gcaaaagaaa
     4981 ttattgttca gcatggaggg cagatttacg cggaaagcaa tgataactat acgacgttta
     5041 gggtagagct tccagggttc tgttgcaaag ttttaaataa agaataaaat ccc
//
LOCUS       DACGLE010000218         3970 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0534
            SAMEA3304449-rid9560453.guided.138, whole genome shotgun sequence.
ACCESSION   DACGLE010000218 DACGLE010000000
VERSION     DACGLE010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304449
            Sequence Read Archive: ERR985064
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3970)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3970)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702225.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702225.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 20:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,045
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 27 of 67
            Pseudo Genes (incomplete)         :: 25 of 67
            Pseudo Genes (internal stop)      :: 19 of 67
            Pseudo Genes (multiple problems)  :: 4 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3970
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304449-rid9560453.guided.138"
                     /strain="VREN0534"
                     /db_xref="taxon:1352"
     gene            22..1941
                     /gene="tet(M)"
                     /locus_tag="IXT20_003029"
     CDS             22..1941
                     /gene="tet(M)"
                     /locus_tag="IXT20_003029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019117172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HAQ4861386.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLYPVYH
                     GSAKSNIGIEQLIEVITNKFYSSTYRKKSELCGNVFKIEYSEERQRLAYVRLYGGILH
                     LRDSVRISEKEKIKITEMYTSINGELCKIDKAYSGEIVILQNEFLKLNSVLGDTKLLP
                     QRERIENPLPLLQTTVEPSKPQQREMLLDALLEISDSDPLLQYYVDSTTHEIILSFLG
                     KVQMEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVS
                     PLPLGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYS
                     PVSTPADFRMLAPIVLEQVLKKAGTELLEPYLSFKIYAPQEYLSRAYNDAPKYCANIV
                     DTQLKNNEVILSGEIPARCIQEYRSDLTFFTNGRSVCLTELKGYHVTTGEPVCQPRRP
                     NSRIDKVRYMFNKIT"
     gene            2039..3412
                     /gene="tet(L)"
                     /locus_tag="IXT20_003030"
     CDS             2039..3412
                     /gene="tet(L)"
                     /locus_tag="IXT20_003030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002387933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(L)"
                     /protein_id="HAQ4861387.1"
                     /translation="MKCNECNRVKYNQILIWLCVLSFFSVLNEMVLNVSLPDIANDFN
                     KPPASTNWVNTAFMLTFSIGTAVYGKLSDQLGIKRLLLFGIIINCFGSVIGFVGHSFF
                     SLLIMARFIQGAGAAAFPALVMVVVARYIPKENRGKAFGLIGSIVAMGEGVGPAIGGM
                     IAHYIHWSYLLLIPMITIITVPFLMKLLKKEVRIKGHFDIKGIILMSVGIVFFMLFTT
                     SYSISFLIVSVLSFLIFVKHIRKVTDPFVDPGLGKNIPFMIGVLCGGIIFGTVAGFVS
                     MVPYMMKDVHQLSTAEIGSVIIFPGTMSVIIFGYIGGILVDRRGPLYVLNIGVTFLSV
                     SFLTASFLLETTSWFMTIIIVFVLGGLSFTKTVISTIVSSSLKQQEAGAGMSLLNFTS
                     FLSEGTGIAIVGGLLSIPLLDQRLLPMEVDQSTYLYSNLLLLFSGIIVISWLVTLNVY
                     KHSQRDF"
BASE COUNT         1254 a          613 c          818 g         1285 t
ORIGIN      
        1 ttttgaatgg aggaaaatca catgaaaatt attaatattg gagttttagc tcatgttgat
       61 gcaggaaaaa ctaccttaac agaaagctta ttatataaca gtggagcgat tacagaatta
      121 ggaagcgtgg acaaaggtac aacgaggacg gataatacgc ttttagaacg tcagagagga
      181 attacaattc agacaggaat aacctctttt cagtgggaaa atacgaaggt gaacatcata
      241 gacacgccag gacatatgga tttcttagca gaagtatatc gttcattatc agttttagat
      301 ggggcaattc tactgatttc tgcaaaagat ggcgtacaag cacaaactcg tatattattt
      361 catgcactta ggaaaatggg gattcccaca atctttttta tcaataagat tgaccaaaat
      421 ggaattgatt tatcaacggt ttatcaggat attaaagaga aactttctgc cgaaattgta
      481 atcaaacaga aggtagaact gtatcctaat atgtgtgtga cgaactttac cgaatctgaa
      541 caatgggata cggtaataga gggaaacgat gaccttttag agaaatatat gtccggtaaa
      601 tcattagaag cattggaact cgaacaagag gaaagcataa gatttcagaa ttgctccttg
      661 taccctgttt atcatggaag cgcaaaaagc aacataggga ttgagcagct tatagaagtg
      721 ataacgaata aattttattc atcaacatac agaaagaagt ctgaactttg cggaaatgtc
      781 ttcaaaattg aatattcgga agaaagacaa cgtcttgcat atgtacgcct ttatggcgga
      841 atcctgcatt tgcgggattc ggttagaata tcggaaaagg aaaaaataaa aattacagaa
      901 atgtatactt caataaatgg tgaattatgt aaaattgata aggcttattc cggggaaatt
      961 gttattttgc aaaatgagtt tttgaagcta aatagtgttc ttggagatac aaagctattg
     1021 ccacagagag agagaattga aaatccgctc cctctgctgc aaacaactgt tgaaccgagc
     1081 aaacctcaac aaagggaaat gttacttgat gcacttttag aaatctccga cagtgacccg
     1141 cttctacaat attatgtgga ttctacgaca catgaaatta tactttcttt cttagggaaa
     1201 gtacaaatgg aagtgattag tgcactgttg caagaaaagt atcatgtgga gatagaacta
     1261 aaagagccta cagtcattta tatggagaga ccgttaaaaa atgcagaata taccattcac
     1321 atcgaagtgc cgccaaatcc tttctgggct tccattggtt tatctgtatc accgcttccg
     1381 ttgggaagtg gaatgcagta tgagagctcg gtttctcttg gatacttaaa tcaatcattt
     1441 caaaatgcag ttatggaagg gatacgctat ggttgcgaac aaggattata tggttggaat
     1501 gtgacggatt gtaaaatctg ttttaagtat ggcttatact atagccctgt tagtacccca
     1561 gcagattttc ggatgcttgc tcctattgta ttggaacaag tcttaaaaaa agctggaaca
     1621 gaattgttag agccatatct tagttttaaa atttatgcgc cacaggaata tctttcacga
     1681 gcatacaacg atgctcctaa atattgtgcg aacatcgtag acactcaatt gaaaaataat
     1741 gaggtcattc ttagtggaga aatccctgct cggtgtattc aagaatatcg tagtgattta
     1801 actttcttta caaatggacg tagtgtttgt ttaacagagt taaaagggta ccatgttact
     1861 accggtgaac ctgtttgcca gccccgtcgt ccaaatagtc ggatagataa agtacgatat
     1921 atgttcaata aaataactta gtgtatttta tgttgttata taaatatggt ttcttgttaa
     1981 ataagatgaa atatttttta ataaagattt gaattaaagt gtaaaggagg aattaattat
     2041 gaagtgtaat gaatgtaaca gggttaaata caaccaaatt ttaatttggc tttgcgtttt
     2101 atcttttttt agcgtattaa atgaaatggt tttgaacgtc tcattacctg atattgcaaa
     2161 tgattttaat aaaccacctg cgagtacaaa ctgggtgaac acagccttta tgttaacctt
     2221 ttccattgga acagctgtat atggaaagct atctgatcaa ttaggcatca aaaggttact
     2281 cctatttgga attataataa attgtttcgg gtcggtaatt gggtttgttg gccattcttt
     2341 cttttcctta cttattatgg ctcgttttat tcaaggggct ggtgcagctg catttccagc
     2401 actcgtaatg gttgtagttg cgcgctatat tccaaaggaa aataggggta aagcatttgg
     2461 tcttattgga tcgatagtag ccatgggaga aggagtcggt ccagcgattg gtggaatgat
     2521 agcccattat attcattggt cctatcttct actcattcct atgataacaa ttatcactgt
     2581 tccgtttctt atgaaattat taaagaaaga agtaaggata aaaggtcatt ttgatatcaa
     2641 aggaattata ctaatgtctg taggcattgt attttttatg ttgtttacaa catcatatag
     2701 catttctttt cttatcgtta gcgtgctgtc attcctgata tttgtaaaac atatcaggaa
     2761 agtaacagat ccttttgttg atcccggatt agggaaaaat atacctttta tgattggagt
     2821 tctttgtggg ggaattatat ttggaacagt agcagggttt gtctctatgg ttccttatat
     2881 gatgaaagat gttcaccagc taagtactgc cgaaatcgga agtgtaatta ttttccctgg
     2941 aacaatgagt gtcattattt tcggctacat tggtgggata cttgttgata gaagaggtcc
     3001 tttatacgtg ttaaacatcg gagttacatt tctttctgtt agctttttaa ctgcttcctt
     3061 tcttttagaa acaacatcat ggttcatgac aattataatc gtatttgttt taggtgggct
     3121 ttcgttcacc aaaacagtta tatcaacaat tgtttcaagt agcttgaaac agcaggaagc
     3181 tggtgctgga atgagtttgc ttaactttac cagcttttta tcagagggaa caggtattgc
     3241 aattgtaggt ggtttattat ccataccctt acttgatcaa aggttgttac ctatggaagt
     3301 tgatcagtca acttatctgt atagtaattt gttattactt ttttcaggaa tcattgtcat
     3361 tagttggctg gttaccttga atgtatataa acattctcaa agggatttct aaatcgttaa
     3421 gggatcaact ttgggagaga gttcaaaatt gatccttttt ttataacagg aattcaaatc
     3481 tttttgttcc attaaagggc gcgattgctg aataaaagat acgagagacc tctcttgtat
     3541 cttttttatt ttgagtggtt ttgtccgtta cactagaaaa ccgaaagaca ataaaaattt
     3601 tattcttgct gagtctggct ttcggtaagc tagacaaaac ggacaaaata aaaattggca
     3661 agggtttaaa ggtggagatt ttttgagtga tcttctcaaa aaatactacc tgtcccttgc
     3721 tgatttttaa acgagcacga gagcaaaacc cccctttgct gaggtggcag agggcaggtt
     3781 tttttgtttc ttttttctcg taaaaaaaag aaaggtctta aaggttttat ggttttggtc
     3841 ggcactgccg acagcctcgc agagcacaca ctttatgaat ataaagtata gtgtgttata
     3901 ctttacttgg aagtggttgc cggaaagagc gaaaatgcct cacatttgtg ccacctaaaa
     3961 aggagcgatt
//
LOCUS       DACGLE010000219         3970 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0534
            SAMEA3304449-rid9560453.guided.139, whole genome shotgun sequence.
ACCESSION   DACGLE010000219 DACGLE010000000
VERSION     DACGLE010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304449
            Sequence Read Archive: ERR985064
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3970)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3970)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702225.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702225.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 20:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,045
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 27 of 67
            Pseudo Genes (incomplete)         :: 25 of 67
            Pseudo Genes (internal stop)      :: 19 of 67
            Pseudo Genes (multiple problems)  :: 4 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3970
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304449-rid9560453.guided.139"
                     /strain="VREN0534"
                     /db_xref="taxon:1352"
     gene            22..1941
                     /gene="tet(M)"
                     /locus_tag="IXT20_003031"
     CDS             22..1941
                     /gene="tet(M)"
                     /locus_tag="IXT20_003031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019117172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HAQ4861388.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLYPVYH
                     GSAKSNIGIEQLIEVITNKFYSSTYRKKSELCGNVFKIEYSEERQRLAYVRLYGGILH
                     LRDSVRISEKEKIKITEMYTSINGELCKIDKAYSGEIVILQNEFLKLNSVLGDTKLLP
                     QRERIENPLPLLQTTVEPSKPQQREMLLDALLEISDSDPLLQYYVDSTTHEIILSFLG
                     KVQMEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVS
                     PLPLGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYS
                     PVSTPADFRMLAPIVLEQVLKKAGTELLEPYLSFKIYAPQEYLSRAYNDAPKYCANIV
                     DTQLKNNEVILSGEIPARCIQEYRSDLTFFTNGRSVCLTELKGYHVTTGEPVCQPRRP
                     NSRIDKVRYMFNKIT"
     gene            2039..3412
                     /gene="tet(L)"
                     /locus_tag="IXT20_003032"
     CDS             2039..3412
                     /gene="tet(L)"
                     /locus_tag="IXT20_003032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002387933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(L)"
                     /protein_id="HAQ4861389.1"
                     /translation="MKCNECNRVKYNQILIWLCVLSFFSVLNEMVLNVSLPDIANDFN
                     KPPASTNWVNTAFMLTFSIGTAVYGKLSDQLGIKRLLLFGIIINCFGSVIGFVGHSFF
                     SLLIMARFIQGAGAAAFPALVMVVVARYIPKENRGKAFGLIGSIVAMGEGVGPAIGGM
                     IAHYIHWSYLLLIPMITIITVPFLMKLLKKEVRIKGHFDIKGIILMSVGIVFFMLFTT
                     SYSISFLIVSVLSFLIFVKHIRKVTDPFVDPGLGKNIPFMIGVLCGGIIFGTVAGFVS
                     MVPYMMKDVHQLSTAEIGSVIIFPGTMSVIIFGYIGGILVDRRGPLYVLNIGVTFLSV
                     SFLTASFLLETTSWFMTIIIVFVLGGLSFTKTVISTIVSSSLKQQEAGAGMSLLNFTS
                     FLSEGTGIAIVGGLLSIPLLDQRLLPMEVDQSTYLYSNLLLLFSGIIVISWLVTLNVY
                     KHSQRDF"
BASE COUNT         1253 a          613 c          819 g         1285 t
ORIGIN      
        1 ttttgaatgg aggaaaatca catgaaaatt attaatattg gagttttagc tcatgttgat
       61 gcgggaaaaa ctaccttaac agaaagctta ttatataaca gtggagcgat tacagaatta
      121 ggaagcgtgg acaaaggtac aacgaggacg gataatacgc ttttagaacg tcagagagga
      181 attacaattc agacaggaat aacctctttt cagtgggaaa atacgaaggt gaacatcata
      241 gacacgccag gacatatgga tttcttagca gaagtatatc gttcattatc agttttagat
      301 ggggcaattc tactgatttc tgcaaaagat ggcgtacaag cacaaactcg tatattattt
      361 catgcactta ggaaaatggg gattcccaca atctttttta tcaataagat tgaccaaaat
      421 ggaattgatt tatcaacggt ttatcaggat attaaagaga aactttctgc cgaaattgta
      481 atcaaacaga aggtagaact gtatcctaat atgtgtgtga cgaactttac cgaatctgaa
      541 caatgggata cggtaataga gggaaacgat gaccttttag agaaatatat gtccggtaaa
      601 tcattagaag cattggaact cgaacaagag gaaagcataa gatttcagaa ttgctccttg
      661 taccctgttt atcatggaag cgcaaaaagc aacataggga ttgagcagct tatagaagtg
      721 ataacgaata aattttattc atcaacatac agaaagaagt ctgaactttg cggaaatgtc
      781 ttcaaaattg aatattcgga agaaagacaa cgtcttgcat atgtacgcct ttatggcgga
      841 atcctgcatt tgcgggattc ggttagaata tcggaaaagg aaaaaataaa aattacagaa
      901 atgtatactt caataaatgg tgaattatgt aaaattgata aggcttattc cggggaaatt
      961 gttattttgc aaaatgagtt tttgaagcta aatagtgttc ttggagatac aaagctattg
     1021 ccacagagag agagaattga aaatccgctc cctctgctgc aaacaactgt tgaaccgagc
     1081 aaacctcaac aaagggaaat gttacttgat gcacttttag aaatctccga cagtgacccg
     1141 cttctacaat attatgtgga ttctacgaca catgaaatta tactttcttt cttagggaaa
     1201 gtacaaatgg aagtgattag tgcactgttg caagaaaagt atcatgtgga gatagaacta
     1261 aaagagccta cagtcattta tatggagaga ccgttaaaaa atgcagaata taccattcac
     1321 atcgaagtgc cgccaaatcc tttctgggct tccattggtt tatctgtatc accgcttccg
     1381 ttgggaagtg gaatgcagta tgagagctcg gtttctcttg gatacttaaa tcaatcattt
     1441 caaaatgcag ttatggaagg gatacgctat ggttgcgaac aaggattata tggttggaat
     1501 gtgacggatt gtaaaatctg ttttaagtat ggcttatact atagccctgt tagtacccca
     1561 gcagattttc ggatgcttgc tcctattgta ttggaacaag tcttaaaaaa agctggaaca
     1621 gaattgttag agccatatct tagttttaaa atttatgcgc cacaggaata tctttcacga
     1681 gcatacaacg atgctcctaa atattgtgcg aacatcgtag acactcaatt gaaaaataat
     1741 gaggtcattc ttagtggaga aatccctgct cggtgtattc aagaatatcg tagtgattta
     1801 actttcttta caaatggacg tagtgtttgt ttaacagagt taaaagggta ccatgttact
     1861 accggtgaac ctgtttgcca gccccgtcgt ccaaatagtc ggatagataa agtacgatat
     1921 atgttcaata aaataactta gtgtatttta tgttgttata taaatatggt ttcttgttaa
     1981 ataagatgaa atatttttta ataaagattt gaattaaagt gtaaaggagg aattaattat
     2041 gaagtgtaat gaatgtaaca gggttaaata caaccaaatt ttaatttggc tttgcgtttt
     2101 atcttttttt agcgtattaa atgaaatggt tttgaacgtc tcattacctg atattgcaaa
     2161 tgattttaat aaaccacctg cgagtacaaa ctgggtgaac acagccttta tgttaacctt
     2221 ttccattgga acagctgtat atggaaagct atctgatcaa ttaggcatca aaaggttact
     2281 cctatttgga attataataa attgtttcgg gtcggtaatt gggtttgttg gccattcttt
     2341 cttttcctta cttattatgg ctcgttttat tcaaggggct ggtgcagctg catttccagc
     2401 actcgtaatg gttgtagttg cgcgctatat tccaaaggaa aataggggta aagcatttgg
     2461 tcttattgga tcgatagtag ccatgggaga aggagtcggt ccagcgattg gtggaatgat
     2521 agcccattat attcattggt cctatcttct actcattcct atgataacaa ttatcactgt
     2581 tccgtttctt atgaaattat taaagaaaga agtaaggata aaaggtcatt ttgatatcaa
     2641 aggaattata ctaatgtctg taggcattgt attttttatg ttgtttacaa catcatatag
     2701 catttctttt cttatcgtta gcgtgctgtc attcctgata tttgtaaaac atatcaggaa
     2761 agtaacagat ccttttgttg atcccggatt agggaaaaat atacctttta tgattggagt
     2821 tctttgtggg ggaattatat ttggaacagt agcagggttt gtctctatgg ttccttatat
     2881 gatgaaagat gttcaccagc taagtactgc cgaaatcgga agtgtaatta ttttccctgg
     2941 aacaatgagt gtcattattt tcggctacat tggtgggata cttgttgata gaagaggtcc
     3001 tttatacgtg ttaaacatcg gagttacatt tctttctgtt agctttttaa ctgcttcctt
     3061 tcttttagaa acaacatcat ggttcatgac aattataatc gtatttgttt taggtgggct
     3121 ttcgttcacc aaaacagtta tatcaacaat tgtttcaagt agcttgaaac agcaggaagc
     3181 tggtgctgga atgagtttgc ttaactttac cagcttttta tcagagggaa caggtattgc
     3241 aattgtaggt ggtttattat ccataccctt acttgatcaa aggttgttac ctatggaagt
     3301 tgatcagtca acttatctgt atagtaattt gttattactt ttttcaggaa tcattgtcat
     3361 tagttggctg gttaccttga atgtatataa acattctcaa agggatttct aaatcgttaa
     3421 gggatcaact ttgggagaga gttcaaaatt gatccttttt ttataacagg aattcaaatc
     3481 tttttgttcc attaaagggc gcgattgctg aataaaagat acgagagacc tctcttgtat
     3541 cttttttatt ttgagtggtt ttgtccgtta cactagaaaa ccgaaagaca ataaaaattt
     3601 tattcttgct gagtctggct ttcggtaagc tagacaaaac ggacaaaata aaaattggca
     3661 agggtttaaa ggtggagatt ttttgagtga tcttctcaaa aaatactacc tgtcccttgc
     3721 tgatttttaa acgagcacga gagcaaaacc cccctttgct gaggtggcag agggcaggtt
     3781 tttttgtttc ttttttctcg taaaaaaaag aaaggtctta aaggttttat ggttttggtc
     3841 ggcactgccg acagcctcgc agagcacaca ctttatgaat ataaagtata gtgtgttata
     3901 ctttacttgg aagtggttgc cggaaagagc gaaaatgcct cacatttgtg ccacctaaaa
     3961 aggagcgatt
//
LOCUS       DACGLE010000220         3604 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0534
            SAMEA3304449-rid9560453.guided.142, whole genome shotgun sequence.
ACCESSION   DACGLE010000220 DACGLE010000000
VERSION     DACGLE010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304449
            Sequence Read Archive: ERR985064
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3604)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3604)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702225.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702225.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 20:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,045
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 27 of 67
            Pseudo Genes (incomplete)         :: 25 of 67
            Pseudo Genes (internal stop)      :: 19 of 67
            Pseudo Genes (multiple problems)  :: 4 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3604
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304449-rid9560453.guided.142"
                     /strain="VREN0534"
                     /db_xref="taxon:1352"
     gene            complement(<1..164)
                     /locus_tag="IXT20_003033"
     CDS             complement(<1..164)
                     /locus_tag="IXT20_003033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HAQ4861390.1"
                     /translation="MKYILSFCTPKLNYLLAYIRLYSGVLHLRDSVRVSEKEKIKVTE
                     MYTSINGELC"
     gene            394..891
                     /gene="dfrG"
                     /locus_tag="IXT20_003034"
     CDS             394..891
                     /gene="dfrG"
                     /locus_tag="IXT20_003034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HAQ4861391.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
     gene            complement(963..2915)
                     /locus_tag="IXT20_003035"
     CDS             complement(963..2915)
                     /locus_tag="IXT20_003035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insertion element protein"
                     /protein_id="HAQ4861392.1"
                     /translation="MTKLKRLATKEDEIVDVKIPISNEELKDRAKQYDLLTPKRFATR
                     YNKMLFLPTSFKWNGSEYPIQYNYCINPFCCNFGKEQHKFKDVKGKPSRYKMTGSSKD
                     KGHKGMYCNDNPIGRGVSQNCTVTPLSNWSVVEEIKRLIEINSIQDVEPDYQFHKEGC
                     SEEESTPFNEPKQFYKRGKSRGKSQRYQCKACKKFTNVLPKREETTTYHQQKNTILPM
                     FAKMVVGRVSVSRTCDILGIGVGTYYHKLEWLYRRCLEFLERYETQPLQTKKFNEMWL
                     NTDKMHYYLNNVRKKGQGSKKYTGFEDLNMQTYIVVSAEVLSRYVFRSDVAYDWNISM
                     DELNEDTRKFKEDHLNTFSRKNDRLDWSYYPQEPSANDSENRNAYLHELGKITNRSRF
                     VDGLNVDAPYTTTAHYWLIKQMVNADEWRMISDDDFSIRNAFYRVFTKELRLSDAHHF
                     ICQVNKTKSRKQCLKEFGQAKAELLDWGDIRGFKTKFLRTLASHYLTELLTSHQFHEE
                     AISKDGERYRKYADNPIKHPLATKDKGFYSVDCRTDLSALEPNEIAKMLLNVNDHSTN
                     SFIQQIRRYISSLERPLTTARGDKKSYIYANFNPKYAQFAITILRTYYNFCRPFKSAD
                     KKVLTPAQRLGITDKQFDWKDIIYFK"
     gene            complement(2922..3218)
                     /locus_tag="IXT20_003036"
     CDS             complement(2922..3218)
                     /locus_tag="IXT20_003036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4861393.1"
                     /translation="MQIGVLKWLQFTKQKGVHFPMQFLEPKNKNAKSVDWEISEQVRV
                     IVKQYAEYAERTESEAVDEFLLNILDDKKFIEWIANKRSNKRIVEKMGIKDRVG"
     gene            complement(3393..>3604)
                     /locus_tag="IXT20_003037"
     CDS             complement(3393..>3604)
                     /locus_tag="IXT20_003037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HAQ4861394.1"
                     /translation="ELEQEESIRFQNCSLFPLYHGSAKSNIGIDNLIEVITNKFYSST
                     HRGPSELCGNVFKIEYTKKRQRLAY"
BASE COUNT         1115 a          622 c          583 g         1284 t
ORIGIN      
        1 ttacataatt caccatttat tgaagtatac atttctgtaa cttttatttt ttctttttct
       61 gatactctaa ccgaatctcg taaatgtagt actccactat aaaggcgtat atatgcaagt
      121 aagtagttca gttttggagt acaaaatgac aaaatgtact tcaaaatgga ctactttttt
      181 cattttaact tacataccaa attacataca aaatgattta taaaatgcaa ttgaaaatga
      241 taattgaatg aaaatttgtg attttgtggt attatgataa tataacttat agaaaaaatt
      301 gcttattcaa ctaacgggca ggataatgag aaaaggtttt ttgatagaaa taagaaatta
      361 gatggtaaat tagttaaaaa ggctgtgtta taaatgaaag tttctttgat tgctgcgatg
      421 gataagaata gagtgattgg caaagagaat gacattcctt ggaggattcc caaggactgg
      481 gaatatgtta aaaatactac aaagggacat ccgataatat taggtaggaa gaaccttgaa
      541 tcaatcggaa gagccttacc tgacagaaga aatattattc tgacgagaga taaggggttt
      601 acctttaatg gttgtgaaat tgttcattca atagaagatg tttttgagtt atgtaaaaac
      661 gaagaagaaa tttttatttt cggaggagaa cagatttata atttgttttt cccttatgtt
      721 gagaaaatgt acatcacaaa aatacatcat gaattcgaag gagatacttt ttttccagaa
      781 gtgaattatg aggaatggaa tgaggtattt gcccaaaaag ggataaagaa tgataaaaat
      841 ccgtataact actattttca tgtatatgaa agaaaaaact tattgagtta acgggtgcgt
      901 taattgaaga agaacagtaa agataaagca caagagcttg ggattactcc aagctctttt
      961 tgtcatttga aataaattat atctttccaa tcaaattgtt tatctgtaat ccctaatctt
     1021 tgagcaggtg tcaaaacctt tttatccgct gatttaaaag ggcgacaaaa gttgtagtaa
     1081 gttcttagaa ttgtaatcgc aaattgagca tacttcggat tgaagttagc gtaaatatag
     1141 ctcttcttat caccacgagc tgttgttaat ggtctttcaa gagatgatat ataacgtctt
     1201 atctgttgaa taaagctatt ggtcgaatgg tcattaacat ttaatagcat ctttgcgatt
     1261 tcgtttggtt ccagagcaga taaatccgtt ctacaatcaa ctgaataaaa tcctttatcc
     1321 ttagtggcta atggatgttt gatagggtta tcggcatatt tgcgatatct ttctccatct
     1381 ttactaatag cctcttcgtg gaattgatga cttgttagta attcagttag ataatgagat
     1441 gccaaagtcc gtagaaactt tgttttgaat cctctgatat ctccccaatc gagcagttct
     1501 gcttttgctt gtccgaattc ttttaagcat tgttttctgc ttttcgtctt gttaacttgg
     1561 caaataaaat gatgtgcatc gctcaacctt aattctttcg tgaatactct ataaaaggcg
     1621 tttctaatag agaagtcatc atcactaatc attcgccatt cgtcggcatt aaccatttgt
     1681 ttaattaacc aatagtgagc agttgtagta taaggagcat caacatttaa accatcgaca
     1741 aatctactcc tattagtaat tttccctaat tcgtgaagat aagcattcct attttcgcta
     1801 tcgttagcgg atggttcttg tggataataa gaccaatcta acctatcatt tttacgacta
     1861 aaagtattga gatggtcttc tttgaatttt cttgtatctt catttaattc atccattgaa
     1921 atattccaat cataggcaac atcagaacgg aacacatatc ttgataagac ctcagcagat
     1981 acaactatat acgtttgcat attaaggtct tcaaaccccg tatatttctt tgaaccttgt
     2041 cctttcttac ggacgttatt taggtagtag tgcatcttgt ccgtattaag ccacatctcg
     2101 ttaaatttct ttgtttgaag gggttgtgtc tcatatcgtt ctaaaaactc caaacaacgc
     2161 ctatacagcc actccagctt atgatagtag gttccaactc caattcctaa aatatcacaa
     2221 gtacgactga ctgaaaccct tccaacaacc atcttagcga acattggaag aatggtgttc
     2281 ttttgttggt gataagtagt ggtttcttct ctttttggta gaacattagt gaacttctta
     2341 cacgccttac attgatagcg ttgagatttg cccctacttt tccccctttt gtaaaattgc
     2401 ttcggctcat tgaatggggt tgattcttct tccgagcagc cttctttatg aaattgataa
     2461 tctggctcaa catcttggat gctattgatt tctataaggc gtttaatttc ttcaacaaca
     2521 gaccaattag ataaaggggt tactgtacag ttttgcgata cacctctgcc aataggattg
     2581 tcattgcagt acataccttt atgaccttta tctttggaac taccagtcat tttatatcga
     2641 cttggtttcc cttttacatc tttgaatttg tgctgttctt tcccaaaatt acaacaaaac
     2701 ggattgatgc agtaattata ttgaatggga tattcagaac cgttccattt aaaagacgtt
     2761 ggcaagaaca acattttgtt ataccttgtt gcaaatcgtt ttggagttaa taaatcatat
     2821 tgtttagccc tatcttttaa ttcttcgttt gaaatcggaa ttttaacatc tacaatttca
     2881 tcttccttcg tggctaatct tttaagcttg gtcattgtct atcaacccac tctatctttg
     2941 atacccattt tttcaacaat ccgcttatta cttcgcttgt tggcaatcca ttcaataaac
     3001 ttcttatcgt ctaaaatgtt caataagaat tcatccactg cttcgctttc agtacgctct
     3061 gcatattcag cgtattgctt aactatcacc cttacttgct ctgatatttc ccaatccaca
     3121 gacttagcgt tcttattttt tggctcaaga aattgcatag gaaaatgcac ccctttttgc
     3181 ttagtaaatt gtaaccattt taacacacca atttgcattc ctacaataat tatttcacgt
     3241 tcgcattttt atatacattt taacttacat tttgtggtgt aaattaacaa caaacgttga
     3301 tttaacaata aaaaacgagt atcaaattca gttgcaattt gatactcatt taggtttaca
     3361 ttttcacttt ttgtactcca aaactgaact acttaatatg caagacgttg tctttttttt
     3421 gtatattcaa ttttgaaaac atttccgcaa agttcagacg gacctcgatg tgttgatgaa
     3481 taaaatttat tagtaataac ttctataagg ttatcaatcc ctatattact ttttgcactt
     3541 ccatgataaa gagggaacag agaacaattc tgaaatctta tgctttcctc ttgttcgagt
     3601 tcca
//
LOCUS       DACGLE010000221         2975 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0534
            SAMEA3304449-rid9560453.guided.150, whole genome shotgun sequence.
ACCESSION   DACGLE010000221 DACGLE010000000
VERSION     DACGLE010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304449
            Sequence Read Archive: ERR985064
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2975)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2975)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702225.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702225.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 20:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,045
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 27 of 67
            Pseudo Genes (incomplete)         :: 25 of 67
            Pseudo Genes (internal stop)      :: 19 of 67
            Pseudo Genes (multiple problems)  :: 4 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2975
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304449-rid9560453.guided.150"
                     /strain="VREN0534"
                     /db_xref="taxon:1352"
     gene            296..1264
                     /locus_tag="IXT20_003038"
     CDS             296..1264
                     /locus_tag="IXT20_003038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-A"
                     /protein_id="HAQ4861395.1"
                     /translation="MNNIGITVYGCEQDEADAFHALSPRFGVMATIINANVSESNAKS
                     APFNQCISVGHKSEISASILLALKRAGVKYISTRSIGCNHIDTTAAKRMGITVDNVAY
                     SPDSVADYTMMLILMAVRNVKSIVRSVEKHDFRLDSDRGKVLSDMTVGVVGTGQIGKA
                     VIERLRGFGCKVLAYSRSRSIEVNYVPFDELLQNSDIVTLHVPLNTDTHYIISHEQIQ
                     RMKQGAFLINTGRGPLVDTYELVKALENGKLGGAALDVLEGEEEFFYSDCTQKPIDNQ
                     FLLKLQRMPNVIITPHTAYYTEQALRDTVEKTIKNCLDFERRQEHE"
     gene            1257..2288
                     /gene="vanA"
                     /locus_tag="IXT20_003039"
     CDS             1257..2288
                     /gene="vanA"
                     /locus_tag="IXT20_003039"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanA"
                     /protein_id="HAQ4861396.1"
                     /translation="MNRIKVAILFGGCSEEHDVSVKSAIEIAANINKEKYEPLYIGIT
                     KSGVWKMCEKPCAEWENDNCYSAVLSPDKKMHGLLVKKNHEYEINHVDVAFSALHGKS
                     GEDGSIQGLFELSGIPFVGCDIQSSAICMDKSLTYIVAKNAGIATPAFWVINKDDRPV
                     AATFTYPVFVKPARSGSSFGVKKVNSADELDYAIESARQYDSKILIEQAVSGCEVGCA
                     VLGNSAALVVGEVDQIRLQYGIFRIHQEVEPEKGSENAVITVPADLSAEERGRIQETA
                     KKIYKALGCRGLARVDMFLQDNGRIVLNEVNTLPGFTSYSRYPRMMAAAGIALPELID
                     RLIVLALKG"
     gene            2294..2902
                     /locus_tag="IXT20_003040"
     CDS             2294..2902
                     /locus_tag="IXT20_003040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-A"
                     /protein_id="HAQ4861397.1"
                     /translation="MEIGFTFLDEIVHGVRWDAKYATWDNFTGKPVDGYEVNRIVGTY
                     ELAESLLKAKELAATQGYGLLLWDGYRPKRAVNCFMQWAAQPENNLTKESYYPNIDRT
                     EMISKGYVASKSSHSRGSAIDLTLYRLDTGELVPMGSRFDFMDERSHHAANGISCNEA
                     QNRRRLRSIMENSGFEAYSLEWWHYVLRDEPYPNSYFDFPVK"
BASE COUNT          885 a          568 c          719 g          803 t
ORIGIN      
        1 cttatttgat agtagattta aaactttgca acagaaccgc ttccagcgat gccagacttg
       61 gttgataaaa ggaggtccta agagatgtat ataatttttt aggaaaatct caaggttatc
      121 tttacttttt cttaggaaat taacaattta atattaagaa acggctcgtt cttacacggt
      181 agacttaata ccgtaagaac gagccgtttt cgttcttcag agaaagattt gacaagatta
      241 ccattggcat ccccgtttta tttggtgcct ttcacagaaa gggttggtct taattatgaa
      301 taacatcggc attactgttt atggatgtga gcaggatgag gcagatgcat tccatgctct
      361 ttcgcctcgc tttggcgtta tggcaacgat aattaacgcc aacgtgtcgg aatccaacgc
      421 caaatccgcg cctttcaatc aatgtatcag tgtgggacat aaatcagaga tttccgcctc
      481 tattcttctt gcgctgaaga gagccggtgt gaaatatatt tctacccgaa gcatcggctg
      541 caatcatata gatacaactg ctgctaagag aatgggcatc actgtcgaca atgtggcgta
      601 ctcgccggat agcgttgccg attatactat gatgctaatt cttatggcag tacgcaacgt
      661 aaaatcgatt gtgcgctctg tggaaaaaca tgatttcagg ttggacagcg accgtggcaa
      721 ggtactcagc gacatgacag ttggtgtggt gggaacgggc cagataggca aagcggttat
      781 tgagcggctg cgaggatttg gatgtaaagt gttggcttat agtcgcagcc gaagtataga
      841 ggtaaactat gtaccgtttg atgagttgct gcaaaatagc gatatcgtta cgcttcatgt
      901 gccgctcaat acggatacgc actatattat cagccacgaa caaatacaga gaatgaagca
      961 aggagcattt cttatcaata ctgggcgcgg tccacttgta gatacctatg agttggttaa
     1021 agcattagaa aacgggaaac tgggcggtgc cgcattggat gtattggaag gagaggaaga
     1081 gtttttctac tctgattgca cccaaaaacc aattgataat caatttttac ttaaacttca
     1141 aagaatgcct aacgtgataa tcacaccgca tacggcctat tataccgagc aagcgttgcg
     1201 tgataccgtt gaaaaaacca ttaaaaactg tttggatttt gaaaggagac aggagcatga
     1261 atagaataaa agttgcaata ctgtttgggg gttgctcaga ggagcatgac gtatcggtaa
     1321 aatctgcaat agagatagcc gctaacatta ataaagaaaa atacgagccg ttatacattg
     1381 gaattacgaa atctggtgta tggaaaatgt gcgaaaaacc ttgcgcggaa tgggaaaacg
     1441 acaattgcta ttcagctgta ctctcgccgg ataaaaaaat gcacggatta cttgttaaaa
     1501 agaaccatga atatgaaatc aaccatgttg atgtagcatt ttcagctttg catggcaagt
     1561 caggtgaaga tggatccata caaggtctgt ttgaattgtc cggtatccct tttgtaggct
     1621 gcgatattca aagctcagca atttgtatgg acaaatcgtt gacatacatc gttgcgaaaa
     1681 atgctgggat agctactccc gccttttggg ttattaataa agatgatagg ccggtggcag
     1741 ctacgtttac ctatcctgtt tttgttaagc cggcgcgttc aggctcatcc ttcggtgtga
     1801 aaaaagtcaa tagcgcggac gaattggact acgcaattga atcggcaaga caatatgaca
     1861 gcaaaatctt aattgagcag gctgtttcgg gctgtgaggt cggttgtgcg gtattgggaa
     1921 acagtgccgc gttagttgtt ggcgaggtgg accaaatcag gctgcagtac ggaatctttc
     1981 gtattcatca ggaagtcgag ccggaaaaag gctctgaaaa cgcagttata accgttcccg
     2041 cagacctttc agcagaggag cgaggacgga tacaggaaac ggcaaaaaaa atatataaag
     2101 cgctcggctg tagaggtcta gcccgtgtgg atatgttttt acaagataac ggccgcattg
     2161 tactgaacga agtcaatact ctgcccggtt tcacgtcata cagtcgttat ccccgtatga
     2221 tggccgctgc aggtattgca cttcccgaac tgattgaccg cttgatcgta ttagcgttaa
     2281 aggggtgata agcatggaaa taggatttac ttttttagat gaaatagtac acggtgttcg
     2341 ttgggacgct aaatatgcca cttgggataa tttcaccgga aaaccggttg acggttatga
     2401 agtaaatcgc attgtaggga catacgagtt ggctgaatcg cttttgaagg caaaagaact
     2461 ggctgctacc caagggtacg gattgcttct atgggacggt taccgtccta agcgtgctgt
     2521 aaactgtttt atgcaatggg ctgcacagcc ggaaaataac ctgacaaagg aaagttatta
     2581 tcccaatatt gaccgaactg agatgatttc aaaaggatac gtggcttcaa aatcaagcca
     2641 tagccgcggc agtgccattg atcttacgct ttatcgatta gacacgggtg agcttgtacc
     2701 aatggggagc cgatttgatt ttatggatga acgctctcat catgcggcaa atggaatatc
     2761 atgcaatgaa gcgcaaaatc gcagacgttt gcgctccatc atggaaaaca gtgggtttga
     2821 agcatatagc ctcgaatggt ggcactatgt attaagagac gaaccatacc ccaatagcta
     2881 ttttgatttc cccgttaaat aaacttttaa ccgttgcacg gacaaactat ataagcggtt
     2941 ctgttgcaaa gttttaaata aagaataaaa tccct
//
LOCUS       DACGLE010000222         2556 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0534
            SAMEA3304449-rid9560453.guided.160, whole genome shotgun sequence.
ACCESSION   DACGLE010000222 DACGLE010000000
VERSION     DACGLE010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304449
            Sequence Read Archive: ERR985064
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2556)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2556)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702225.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702225.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 20:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,045
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 27 of 67
            Pseudo Genes (incomplete)         :: 25 of 67
            Pseudo Genes (internal stop)      :: 19 of 67
            Pseudo Genes (multiple problems)  :: 4 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2556
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304449-rid9560453.guided.160"
                     /strain="VREN0534"
                     /db_xref="taxon:1352"
     gene            <1..324
                     /locus_tag="IXT20_003041"
     CDS             <1..324
                     /locus_tag="IXT20_003041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000393259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ4861398.1"
                     /translation="GFYSLFKTLQQNQIIGFAYCYTLLRPDGKTMFYLHSIGMLPNYQ
                     DKGYGSKLLSFIKEYSKEIGCSEMFLITDKGNPRACHVYEKLGGKNDYKDEIVYVYDY
                     EKGDK"
     gene            325..1764
                     /locus_tag="IXT20_003042"
                     /note="aph(2'')-Ia"
     CDS             325..1764
                     /locus_tag="IXT20_003042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(2'')-Ia"
                     /protein_id="HAQ4861399.1"
                     /translation="MNIVENEICIRTLIDDDFPLMLKWLTDERVLEFYGGRDKKYTLE
                     SLKKHYTEPWEDEVFRVIIEYNNVPIGYGQIYKMYDELYTDYHYPKTDEIVYGMDQFI
                     GEPNYWSKGIGTRYIKLIFEFLKKERNANAVILDPHKNNPRAIRAYQKSGFRIIEDLP
                     EHELHEGKKEDCYLMEYRYDDNATNVKAMKYLIEHYFDNFKVDSIEIIGSGYDSVAYL
                     VNNEYIFKTKFSTNKKKGYAKEKAIYNFLNTNLETNVKIPNIEYSYISDELSILGYKE
                     IKGTFLTPEIYSTMSEEEQNLLKRDIASFLRQMHGLDYTDISECTIDNKQNVLEEYIL
                     LRETIYNDLTDIEKDYIESFMERLNATTVFEGKKCLCHNDFSCNHLLLDGNNRLTGII
                     DFGDSGIIDEYCDFIYLLEDSEEEIGTNFGEDILRMYGNIDIEKAKEYQDIVEEYYPI
                     ETIVYGIKNIKQEFIENGRKEIYKRTYKD"
     gene            complement(1894..>2556)
                     /locus_tag="IXT20_003043"
     CDS             complement(1894..>2556)
                     /locus_tag="IXT20_003043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015077585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAQ4861400.1"
                     /translation="DFILYLKLCNRTSCHIAIGITKDGDREIIGFMIQSGESEETWTT
                     FFEYLKERGLQGTELVISDAHKGLVSAIRKSFTNVSWQRCQVHFLRNIFTTIPKKNSK
                     SFREAVKGIFKFTDINLAREAKNRLIHDYIDQPKYSKACASLDDGFEDAFQYTVQGNS
                     HNRLKSTNLIERLNQEVRRREKIIRIFPNQTSANRLIGAVLMDLHDEWIYSSRKYINF
                     DK"
BASE COUNT          975 a          265 c          445 g          871 t
ORIGIN      
        1 ggattttatt ctttatttaa aactttgcaa cagaaccaaa ttataggatt tgcatattgc
       61 tatacacttt taagacctga tggaaaaaca atgttttatt tacactcaat aggaatgtta
      121 cctaactatc aagacaaagg ttatggttca aaattattat cttttattaa ggaatattct
      181 aaagagattg gttgttctga aatgttttta ataactgata aaggtaatcc tagagcttgc
      241 catgtatatg aaaaattagg tggtaaaaat gattataaag atgaaatagt atatgtatat
      301 gattatgaaa aaggtgataa ataaatgaat atagttgaaa atgaaatatg tataagaact
      361 ttaatagatg atgattttcc tttgatgtta aaatggttaa ctgatgaaag agtattagaa
      421 ttttatggtg gtagagataa aaaatataca ttagaatcat taaaaaaaca ttatacagag
      481 ccttgggaag atgaagtttt tagagtaatt attgaatata acaatgttcc tattggatat
      541 ggacaaatat ataaaatgta tgatgagtta tatactgatt atcattatcc aaaaactgat
      601 gagatagtct atggtatgga tcaatttata ggagagccaa attattggag taaaggaatt
      661 ggtacaagat atattaaatt gatttttgaa tttttgaaaa aagaaagaaa tgctaatgca
      721 gttattttag accctcataa aaataatcca agagcaataa gggcatacca aaaatctggt
      781 tttagaatta ttgaagattt gccagaacat gaattacacg agggcaaaaa agaagattgt
      841 tatttaatgg aatatagata tgatgataat gccacaaatg ttaaggcaat gaaatattta
      901 attgagcatt actttgataa tttcaaagta gatagtattg aaataatcgg tagtggttat
      961 gatagtgtgg catatttagt taataatgaa tacattttta aaacaaaatt tagtactaat
     1021 aagaaaaaag gttatgcaaa agaaaaagca atatataatt ttttaaatac aaatttagaa
     1081 actaatgtaa aaattcctaa tattgaatat tcgtatatta gtgatgaatt atctatacta
     1141 ggttataaag aaattaaagg aactttttta acaccagaaa tttattctac tatgtcagaa
     1201 gaagaacaaa atttgttaaa acgagatatt gccagttttt taagacaaat gcacggttta
     1261 gattatacag atattagtga atgtactatt gataataaac aaaatgtatt agaagagtat
     1321 atattgttgc gtgaaactat ttataatgat ttaactgata tagaaaaaga ttatatagaa
     1381 agttttatgg aaagactaaa tgcaacaaca gtttttgagg gtaaaaagtg tttatgccat
     1441 aatgatttta gttgtaatca tctattgtta gatggcaata atagattaac tggaataatt
     1501 gattttggag attctggaat tatagatgaa tattgtgatt ttatatactt acttgaagat
     1561 agtgaagaag aaataggaac aaattttgga gaagatatat taagaatgta tggaaatata
     1621 gatattgaga aagcaaaaga atatcaagat atagttgaag aatattatcc tattgaaact
     1681 attgtttatg gaattaaaaa tattaaacag gaatttatcg aaaatggtag aaaagaaatt
     1741 tataaaagga cttataaaga ttgattatat aatatatgaa aagctattat aaaagacatt
     1801 agtattaaat agtttaaaaa aatgaaaaat aataaaggaa gtgagtcaag tccagactcc
     1861 tgtgtaaaat gctatacaat gtttttacca tttctactta tcaaaattga tgtattttct
     1921 tgaagaataa atccattcat catgtaggtc cataagaacg gctccaatta agcgattggc
     1981 tgatgtttga ttggggaaga tgcgaataat cttttctctt ctgcgtactt cttgattcag
     2041 tcgttcaatt agattggtac tctttagtcg attgtgggaa tttccttgta cggtatattg
     2101 aaaggcgtct tcgaatccat catccaatga tgcgcaagct tttgaatatt ttggttgatc
     2161 gatataatca tgaatcaatc gatttttagc ctcacgcgct aagttaatat ctgtgaactt
     2221 aaaaattcct ttaacagctt ctctgaaaga ttttgaattt tttttaggaa tggtggtaaa
     2281 gatatttctt aggaagtgaa cttggcatct ttgccaactt acgttggtga aggattttct
     2341 aatggcagag actaatcctt tgtgcgcatc agaaataacg agttccgtac cttgtaaacc
     2401 gcgttctttt aggtattcaa aaaatgttgt ccaggtctct tcgctttcgc cactttgaat
     2461 catgaagccg ataatttcac ggtcgccatc tttggttatt ccaatcgcta tatgacagct
     2521 ggttctgttg caaagtttta aataaagaat aaaatc
//
LOCUS       DACGLE010000223         1041 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0534
            SAMEA3304449-rid9560453.guided.191, whole genome shotgun sequence.
ACCESSION   DACGLE010000223 DACGLE010000000
VERSION     DACGLE010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304449
            Sequence Read Archive: ERR985064
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1041)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1041)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702225.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702225.1
            Genome Coverage       :: 157x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 20:36:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,045
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,919
            CDSs (with protein)               :: 2,919
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 27 of 67
            Pseudo Genes (incomplete)         :: 25 of 67
            Pseudo Genes (internal stop)      :: 19 of 67
            Pseudo Genes (multiple problems)  :: 4 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1041
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304449-rid9560453.guided.191"
                     /strain="VREN0534"
                     /db_xref="taxon:1352"
     gene            84..143
                     /gene="ermCL"
                     /locus_tag="IXT20_003044"
     CDS             84..143
                     /gene="ermCL"
                     /locus_tag="IXT20_003044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002497446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methylase leader peptide ErmCL"
                     /protein_id="HAQ4861401.1"
                     /translation="MGIFSIFVINTVHYQPNKK"
     gene            203..937
                     /locus_tag="IXT20_003045"
     CDS             203..937
                     /locus_tag="IXT20_003045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011282421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(T)"
                     /protein_id="HAQ4861402.1"
                     /translation="MNKKNIKDSQNFITSKHHINEILRNVHLNTNDNIIEIGSGKGHF
                     TFELAKRCNYVTAIEIDPKLCRITKNKLIEYENFQVINKDILQFKFPKNKSYKIYGNI
                     PYNISTDIIRKIVFESTATESYLIVEYGFAKRLLNTNRSLALFLMTEVDISILSKIPR
                     EYFHPKPRVNSSLIVLKRHPSKISLKDRKQYENFVMKWVNKEYIKLFSKNQFYQALKY
                     ARIDDLNNISFEQFLSLFNSYKLFNR"
BASE COUNT          419 a          138 c          138 g          346 t
ORIGIN      
        1 gattttattc tttatttaaa actttgcaac agaaccagct tgtgctataa ttattctaat
       61 ttcataaaaa ggagaattaa tatatgggca tttttagtat ttttgtaatc aacacagttc
      121 attatcaacc aaacaaaaaa taagtggtta taatggattg ttaatataaa cattcattat
      181 aacctcatag gagtgggtta taatgaacaa aaaaaatata aaagatagtc aaaactttat
      241 tacttcaaag catcatataa atgaaatttt gagaaatgta catttaaata caaatgataa
      301 tattattgag attggttcag ggaaaggtca tttcacgttt gaattagcta aaaggtgtaa
      361 ttatgtaacc gccattgaaa tagatcctaa attatgtagg ataactaaaa acaaacttat
      421 tgaatatgag aacttccagg ttatcaataa agatatttta caatttaagt ttcctaaaaa
      481 taagtcatat aagatatatg gaaatatacc ctacaatata agtacagata taattcgaaa
      541 aattgttttt gaaagcacag ctacagaaag ttatttaata gtggaatatg gatttgctaa
      601 aaggttgcta aatacaaatc gttcactagc actattttta atgacagaag ttgatatatc
      661 catattaagt aaaatcccta gagaatactt tcatccaaaa cctagagtta atagctcgtt
      721 aattgtatta aaaagacacc cttcaaaaat atcactcaaa gatagaaaac aatatgaaaa
      781 ttttgttatg aaatgggtta acaaagaata cataaaacta ttttccaaaa accaatttta
      841 tcaagcctta aaatatgcaa gaattgacga tttaaacaat attagctttg aacaattctt
      901 gtctcttttc aatagctata aattatttaa tagataagtt aaggggtgca taaactgcat
      961 cccttaactt atcttatttt gcttaaacaa tcttggcccg taaactgcat aactcatatg
     1021 taaatcgctc ctttttaggt g
//
